LOCUS       DATLFO010000001         2639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_49594, whole genome shotgun sequence.
ACCESSION   DATLFO010000001 DATLFO010000000
VERSION     DATLFO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49594"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1336)
                     /locus_tag="VM841_00005"
     CDS             complement(170..1336)
                     /locus_tag="VM841_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL88592.1"
                     /translation="MLSELERLTARIAEIAAGAPARRDDPVAWLDAFERLGRVAAAGR
                     LAALDLIDRTRAWQGEGHASMAHWTAERTGTTVGHAVGAVTAAKALSGLTATREALVS
                     GRLSVQQTSEIATAAAADPGSEGALLGEALRSSAIELREKCRMVRVAAAGDDAYERVR
                     RTRYFRHRLEDDGAVRVDARLPAEDAAPLLMRCHADADRRSARARRDGTREPYEAHMA
                     DALCVLARSPSDSDAAAAPRTTVMVHVDAAAWERGHARPGERCEIAGVGPTTVAAARR
                     LAARPGGTLKIAARSGGNVTGVVSTGRYVPALVDSALEARDERCVIKGCTRRRGLERD
                     HIVEFSRGGETSVANLQRLCGYHHSLKTHKGWRIVGTPPNCRMVPPKPEPDAPP"
     gene            complement(1409..>2639)
                     /locus_tag="VM841_00010"
     CDS             complement(1409..>2639)
                     /locus_tag="VM841_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HVL88593.1"
                     /translation="FRRFVVDLDGVVWSGTSPIPGSCETIRALRDAGCRFAFVTNNSS
                     ERPEVFAAKLESMGAGGDPSEVVTSAMATGKLLETAFEDVRGRTAFVVGGPGLVEAVR
                     AAGVHPIEGDAALDVSMVVVGLDVGLTYEKLRIATLAIRRGARFVAANVDPTLPAEEG
                     QRPGAGAIVAALQATTGVTPLVAGKPEPYILQIAQEKLDGGPALVVGDRISTDVIAAK
                     ALGWPSALVLTGATTLAELAAAPVWPDAILRRMSDLLEDLPHPRVRPASGPDLPVVAT
                     LLHEGGLQAGNVRERSGRTVVAENGRDTLIATAAWDPAGESVALVRSVAVSPGCRGKT
                     AGILAVAGALRLAARAGMREAYLATVDAEGFFTRCGFATVDRDVVPESVLAHPQISRE
                     CPTSAAVMKLTIPDMSS"
BASE COUNT          400 a         1020 c          858 g          361 t
ORIGIN      
        1 gatatcacca ctccctcaag acgcacaaag ggtggcggat cgtcgggacg cctccgaatt
       61 gccgcatggt tccgccgaag ccggagccgg atgcaccgcc gtagcggcgc gccggaaaat
      121 cactccggca cgcgttcaac ccgtgccgga gtgattttcc ggcgcgccgc tacggcggtg
      181 catccggctc cggcttcggc ggaaccatgc ggcaattcgg aggcgtcccg acgatccgcc
      241 accctttgtg cgtcttgagg gagtggtgat atccgcacag gcgctgcaga ttcgcgaccg
      301 acgtctcgcc gccgcgcgag aactcgacga tgtgatcacg ttccagcccg cgccgacgcg
      361 tgcagccttt gatcacgcac cgctcgtcgc gtgcctccag cgctgaatct accaatgccg
      421 gcacgtaccg gccggtgctg accacgccgg tcacgttccc accgctgcgt gccgcgatct
      481 tgagcgtgcc gcccggccgt gccgccagac gccgggccgc cgccaccgtc gtcggcccga
      541 ctcccgcaat ctcgcaccgc tcccccggcc gggcgtgtcc gcgctcccaa gccgcggcgt
      601 ccacgtgcac catcaccgtg gtgcgcggcg ccgcggcggc gtcagaatcc gacgggctgc
      661 gagccaagac gcacaacgcg tcggccatat gagcctcgta cggctctcgc gttccgtcgc
      721 gtcgagcgcg cgctgatcgc cggtccgcat ctgcgtggca tcgcatcagc agcggcgcgg
      781 cgtcttcggc cggcagccgc gcgtccacgc gcacagcccc gtcgtcttca agccggtgac
      841 ggaagtaccg tgtccgccga acccgctcgt aggcatcgtc gcccgccgcc gcgacgcgaa
      901 ccatgcggca tttctcgcgc agctcgatcg cggaagaacg cagtgcttcg ccgagcagcg
      961 caccttcgga gccgggatcc gccgcggccg cggtcgcgat ctcggacgtc tgctgaaccg
     1021 acaggcgccc cgaaacgagc gcctcccgcg tcgcggtcaa gccgctcagc gctttcgcgg
     1081 ccgtcaccgc gcctaccgcg tgtccaaccg tcgtgccggt ccgctcggcc gtccagtgcg
     1141 ccatcgacgc gtgaccttcg ccctgccacg cgcgcgtccg gtcgatcaga tccagcgcgg
     1201 ccagccgccc ggctgccgcg acccggccca gccgctcgaa cgcgtccagc cacgcaaccg
     1261 ggtcatcgcg ccgggccggc gcccccgcgg cgatctccgc gatccgggcg gtcagccgct
     1321 ccagctccga aagcatgccc cgagtatcga acggacgttc ggccgggtcc attccgggaa
     1381 gaacgagcag aaacgggctc gcgagacttt acgaagacat gtccgggatc gtcagcttca
     1441 tcaccgcggc cgacgtcggg cactcgcgag agatctgcgg atgggccagg acggactcgg
     1501 gaacgacgtc gcgatccacc gtcgcgaagc cgcaccgggt gaagaagcct tcggcatcga
     1561 ccgtggccag atacgcctct cgcattcccg cccgggccgc cagtcgcaac gcccccgcga
     1621 ccgcaaggat gcccgcggtc ttgccccgac accccggcga caccgcgacc gatcgcacca
     1681 acgcgaccga ctcgccggcg ggatcccacg cggcggtcgc gatgagcgtg tcccggccgt
     1741 tttcggccac gacggtgcgg ccggatcgtt cccgcacgtt gccggcctga aggccgccct
     1801 cgtgaagcaa cgtcgccacg accggcaggt ccgggcccga tgcaggccgc acccgcgggt
     1861 gcggaaggtc ctccaacaag tccgacatcc ggcgaaggat cgcgtcgggc cacaccggag
     1921 ccgcggccag ctccgccagc gtggtcgccc cggtcagcac cagcgcgctc ggccacccga
     1981 gcgctttggc cgctatcacg tcggtcgaga tccggtcgcc gaccaccagc gcgggtccgc
     2041 cgtcgagctt ctcctgcgcg atctgcagga tgtagggttc gggcttgccg gccacgagtg
     2101 gagtgacgcc cgtcgtcgcc tgcagcgccg cgacgatcgc cccggccccc ggccgctgcc
     2161 cttcctcggc gggcaacgtc ggatcgacgt tggccgccac gaaccgggcg ccccggcgga
     2221 tcgcgagcgt ggcgatgcga agcttttcgt aggtcaggcc gacgtcgagc ccgacgacga
     2281 ccatcgaaac gtccaacgcg gcgtcgcctt cgatcgggtg cacgcccgcg gcccggaccg
     2341 cttccacgag acccggtccg ccgacgacga acgccgttcg cccgcgcacg tcctcgaagg
     2401 cggtctcgag cagcttgccg gtcgccatcg ccgaggtcac gacctcggag gggtcgcctc
     2461 cggctcccat cgactcgagc ttggcggcga agacctccgg ccgctccgag gagttgttgg
     2521 tgacgaaggc gaaccggcaa cccgcgtcgc gcagcgcgcg gatggtctcg cacgagcccg
     2581 ggatcggcga ggtgcccgac cacaccaccc cgtccagatc gacgacgaaa cgccggaac
//
LOCUS       DATLFO010000002         5404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590 k141_53,
            whole genome shotgun sequence.
ACCESSION   DATLFO010000002 DATLFO010000000
VERSION     DATLFO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VM841_00015"
     CDS             <1..460
                     /locus_tag="VM841_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88594.1"
                     /translation="AVDEAGDRVGREATEETVERAGRETAESFPRERVEELADDPSVG
                     GITDKTLREAEVGLSLEQRGTLRGPIRRDPDGFGEFIDADDVVWDVKAFHSGHPKGFE
                     LNKAMRNITREIRVGEKVIVDTLNMSEEHVRLLEEAITQQGLSEHVVFHR"
     gene            457..1074
                     /locus_tag="VM841_00020"
     CDS             457..1074
                     /locus_tag="VM841_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVL88595.1"
                     /translation="MTTAWISDALAAHKEWVASAGAAGKRLSEVGLDLSGADLSGAVL
                     HDCVLPEAKLSGATLDRADLSSGFLNGATLDGASLRNAVLVKAQFDDTSLIGSVLTGA
                     NCLRTTFDGAMLRDANLMQASLRKTAFSNTDLRNANLAGSHIHNTFFDNGTKVAGLIL
                     WRAEICSFHVNWIDIAEEGDPQRLDGEEAEQWIRARATIAPQRRL"
     gene            complement(1554..2966)
                     /locus_tag="VM841_00025"
     CDS             complement(1554..2966)
                     /locus_tag="VM841_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HVL88596.1"
                     /translation="MISEAAAYLLLGATLLLLLWIGVGARARTETLDDYITARNSQTG
                     VALGLSFFASGMGAWILFAPPEVGAFIGIAGIVGYAVAAAAPFLVFALLGPRIRRIVP
                     RGHGLTEFARLRFGRTFHLYVVGLSILYMLVFVVAELTAIGGVAAIVAGIDGRVAVAA
                     VAATTLAYTAYGGLRASLRTDGWQAWMIFALLTAAAVAIFRDGVSSSASFRASGLLGV
                     GRPGLEAAATLVVAVIAANMFHQGYWQRIWSARDERALARGAAFGMATTLPVVLIVGL
                     FGMLAIARGVDLGAPPVPFFALLTGLPAWMGGVILVLGIALVASSVDTLESGLASLVA
                     IERPSLSLGAARMATVVLVVPAVAVAFEGYSVLRIFLIADLLCSTAVVPVLLGLWRRA
                     TPAAALAGSLAGLAGAVLPGGVEAATFPAAIPTLGPFAGALLASSVVAVALSLRARRE
                     TNLDDLDTRVPVLGEAGAQA"
     regulatory      complement(2956..3068)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3086..3712)
                     /locus_tag="VM841_00030"
     CDS             complement(3086..3712)
                     /locus_tag="VM841_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HVL88597.1"
                     /translation="MRVVSVNVGRPQTYSWQGDEITTSIFKSPVEGRVRVGELGLDGD
                     EQANKRVHGGPYKAVYAYAVEELRRLGRMTGHDLEPGGVGENLTLEGVDVRSAEVGER
                     WRVGTACVQVTMPRSPCTKIELRTGDPDFRRKFADARLFGAYLRVVEEGEVGAGDPVE
                     ILHRPGHGLTVTKVFEITAGPRERAAELLVAGDALPPHLRELTARRSI"
     gene            complement(3709..5229)
                     /locus_tag="VM841_00035"
     CDS             complement(3709..5229)
                     /locus_tag="VM841_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVL88598.1"
                     /translation="MGIADKLSGSGSASIQILSGTAQNVVGVGVAALGTLVAQILIGR
                     TLGNAGFGLVTVVTQAAFVTSFLTRAGMDMAVLRTVATATGEGRTNALRTVVARAALI
                     AAAASTVVALAAVAGADALRSLLSLDPIRGRGAIQAAAAGLPFLALANVWLAATRGLK
                     IMRYTLYVFWAGQPLVWIVLMLVGWRVSESPSSAAFAYSTSWAWAAAAAWWCWRRESR
                     SWRTEQPERGATSSLFRYAGPRAPAALFSQVLFWADLFVLTRYVGDAEVGIYSAALRA
                     GQVVVLFLTSVNLMFSPYVADLHNRGEIAQLDRLFKRLTRWTLAATLPGFLLLAVAPA
                     SVLRLFGGTFADGRTALLILLVGQFVNIATGSVGFVLIMAGRTGWDLALYAASLALDV
                     GLALYLCPRYGIAGAAAANAFTFGISNVARLVLVKRLVGIQPYDRRYGRLLAPAVASG
                     AIMMIAHGAVGGGWGTELVATAVAGGLAYVVVFAAVGLTADERRGARVLLTKARSA"
     gene            complement(5220..>5404)
                     /locus_tag="VM841_00040"
     CDS             complement(5220..>5404)
                     /locus_tag="VM841_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HVL88599.1"
                     /translation="YGYPIVLRAVTSDDAMTADWARLEYDLLERVSSRVVNEVPGVNR
                     VVYDITSKPPGTIEWE"
BASE COUNT         1080 a         1815 c         1757 g          752 t
ORIGIN      
        1 ggcggtcgat gaggccgggg accgggtggg gcgggaggcg acggaggaga cagtcgagcg
       61 agccggccgt gaaactgctg aaagctttcc gcgcgaacgc gtcgaagaac ttgccgacga
      121 cccgtcggtc ggcggcatca ccgacaagac cctgcgggag gcagaggtgg gtttgagcct
      181 cgagcaacgc ggcactctgc gcggcccgat ccggcgcgac cccgacggtt ttggagagtt
      241 catagacgcc gacgacgtgg tgtgggacgt gaaggccttc cactctggac atccaaaagg
      301 attcgaactc aataaggcga tgcggaacat cacacgtgag atcagggtcg gcgagaaagt
      361 aatcgtcgat acactgaaca tgagcgaaga gcacgttcga ctgctggagg aagcaatcac
      421 acagcaaggt ctatccgaac atgtggtgtt ccatcgatga caaccgcatg gatctccgac
      481 gcgctcgccg cacataagga atgggttgcg tccgcgggtg ccgccggaaa acgcctatcc
      541 gaggtcggtt tggatctatc cggagcggat ctgtccgggg ccgttctaca cgactgcgtg
      601 cttccggaag cgaagctttc cggagcgaca ctcgatcgcg cggacctgtc ttcaggtttt
      661 ctcaacggtg caacgctcga tggagcaagc ctacgaaacg ccgtcttagt aaaagcccag
      721 ttcgacgaca cctctctaat cggaagcgta ctcacaggag cgaactgctt gcgcacgact
      781 ttcgatggtg caatgctacg cgacgcgaac ttgatgcagg cttctctgag gaagacggcg
      841 ttcagcaata ccgatttgcg caatgccaac cttgcgggca gtcacattca caacacattc
      901 ttcgataacg gtacgaaggt tgcggggctc atcttgtggc gagcagaaat atgctcgttt
      961 cacgtcaact ggatcgatat cgcagaagag ggcgatcctc agcgcctcga cggtgaggag
     1021 gcggagcagt ggattcgggc gcgggcgacg atagcgcctc aacgccggct gtgacatgtc
     1081 gcgcgaggca catgtcttca atcgacgcgg attacgcggt ctcgtcgcag acgaaggaat
     1141 ccgtcgcctg ttactcgtcg acgatctcca acgagaagtg gagcgctatg agcgagggga
     1201 agcggcgatt tgaaatctcc gtattcaccg gcgacccgat gacagagagc aacttgtacc
     1261 tggggctcgt gctgttgagc agctgcagca ccgtccgcaa cctggttgcc atgcacgcca
     1321 accggagaga tacggcggcc aacctatcgc cgcgtcccca tgcgccgttg atcgaatgga
     1381 tcacatttct atccgatcgc catcggacgc cggccggcgt gcccatcaca ctccgcgaag
     1441 ccgaactcga tcgacgtggg agaggacgac gttcctcatc gcctcgaagg tgacgaggca
     1501 gaaggatgga ccgcgcccgg cggcggagac gcggtggcgc aaatcgtgat gccttacgcc
     1561 tgcgcgccgg cctctcccag caccgggacg cgggtatcga ggtcgtcgag gttcgtttcg
     1621 cggcgcgccc tcagtgacag cgcgacggcg acgaccgacg aggccaggag cgcgccggcg
     1681 aacgggccga gggtggggat cgccgccggg aaagtggccg cctccacgcc gccgggtagc
     1741 accgcgccgg cgagcccggc gagcgagccg gcgagcgcgg ccgccggcgt ggcgcggcgc
     1801 cacaacccga gcagcaccgg gacgaccgcg gtcgagcaca acaggtcggc gatgagaaag
     1861 atgcgcagca cggaatagcc ctcgaacgcg accgcgaccg cgggaacgac cagtaccacg
     1921 gtggccatgc gcgccgcccc gagcgacaga gaaggccgtt cgatggcgac caacgacgcc
     1981 agcccgctct cgagcgtgtc aacggacgac gccacgagcg caatgccgag caccaggatg
     2041 accccgccca tccatgccgg cagcccggtc agcaacgcaa agaagggcac cggcggtgcg
     2101 ccgaggtcga cgccgcgcgc gatcgccagc atcccgaaga ggccgacgat cagcacgacc
     2161 ggcagggtcg ttgccattcc gaatgcggcg ccgcgcgcga gcgcgcgctc gtcgcgggcc
     2221 gaccagatcc gctgccagta cccctggtgg aacatgttgg ccgcgatcac ggccacgacg
     2281 agggttgcgg ccgcctcgag gccgggccgc ccgacaccga gcagaccgga agcacgaaac
     2341 gacgccgacg acgacacgcc gtcgcgaaag atcgcgacgg ccgccgccgt caagagtgca
     2401 aagatcatcc acgcctgcca gccgtcggtg cgcagcgacg cacgcaggcc gccatacgcg
     2461 gtgtacgcga gcgtcgtcgc cgcgactgcg gcgaccgcga cgcggccgtc gatacccgca
     2521 acgatcgcgg cgaccccgcc gattgcagtc aactcggcga cgacgaacac cagcatgtag
     2581 aggatcgaga gaccgacgac atagaggtga aacgtccgtc cgaaacgcag ccgcgcgaac
     2641 tcggttagac cgtgcccccg cgggactatg cggcggatgc gcggcccgag caaagcgaag
     2701 accagaaacg gtgcggcggc cgcaaccgca taaccgacga tccccgcgat cccgatgaac
     2761 gcgccgactt ccggcggcgc gaaaagaatc cacgctccca ttcccgacgc gaagaacgac
     2821 agccccagcg cgactcccgt ctgtgagttg cgcgcggtta tgtagtcgtc gagggtctcg
     2881 gtccgggcac gcgccccgac gccgatccac aacagcagca agagagtcgc gccgaggagc
     2941 aaataggcag cggcctccga gatcatcccg cctcccttcg ccggcattac ccggaacagg
     3001 ttcatgcggt cggcggcgca cacagccgcc ctctcagccc ggtagcccga gctcccgcgg
     3061 gtaatgggag attatcaggg cgcggctaaa tggagcggcg cgcggtgagc tcccggaggt
     3121 gaggcgggag ggcgtcgccc gcaacgagca actctgcagc gcgctcgcgc ggcccggccg
     3181 tgatctcgaa caccttggtg acggtgagac cgtgtcccgg ccggtgcagg atctcgaccg
     3241 ggtcgccggc cccgacctct ccctcctcga ccacccgtag gtaggcaccg aacaggcgtg
     3301 catctgcaaa tttgcgccgg aaatcgggat cgccggtgcg gagttcgatc ttcgtacacg
     3361 gcgagcgcgg catcgtcacc tgaacgcaag cggtgccgac gcgccacctc tcgcccactt
     3421 ctgccgaccg gacgtcgacg ccctcgagcg tgaggttctc accgacgccg cccggctcga
     3481 ggtcgtgccc ggtcatcctc ccgagccgcc gcagctcctc gacggcgtaa gcgtagacgg
     3541 ccttgtatgg ccccccatgg acccttttgt tcgcctgctc gtcgccgtcg aggcccagct
     3601 cgcccaccct cacccgtccc tcgaccgggc tcttgaaaat cgaggtggtg atctcatcgc
     3661 cctgccacga gtaagtctgc ggacggccga cgttgaccga cacgactctc atgcactcct
     3721 cgccttcgtc agcagaactc ttgcgccgcg gcgctcgtct gcggtcagcc ccacagctgc
     3781 gaaaacgacc acgtaggcga gacctccggc gaccgccgtg gcgacgagtt ccgttcccca
     3841 gccgccgccg accgctccgt gggcgatcat catgatcgcg ccggacgcga ctgcaggggc
     3901 gagcagccgc ccgtaacggc gatcgtacgg ctgaattccg accagacgct tcaccagcac
     3961 gagccgcgcg acgttcgaaa tgccgaatgt aaacgcgttg gcggcggcgg cgccggcgat
     4021 gccgtagcgg gggcagaggt aaagcgcgag gcccacgtcc agcgcgagcg acgccgcgta
     4081 caaggcgagg tcccagccgg tccgccccgc catgatcaac acgaagccga cgcttcccgt
     4141 tgcgatgttg acgaattgac cgacgaggag aatcagcaac gccgtcctgc cgtccgcgaa
     4201 ggtgccaccg aagagacgca acaccgatgc cggcgccacc gcaagcagca gaaacccggg
     4261 gagcgttgcc gcgagcgtcc agcgcgtgag ccgcttgaaa agtcggtcca gttgtgcgat
     4321 ctcgccgcga ttgtggaggt ccgcgacata gggactgaac atcaggttca ccgacgtgag
     4381 gaacaaaacc accacctgcc cggcgcgcag cgcggccgaa tagatcccga cctcggcatc
     4441 gcccacatag cgagtgagga caaagaggtc cgcccagaac aggacctgcg agaagagcgc
     4501 agcaggagca cgtgggcccg catagcgaaa cagcgacgac gtcgctcccc gctcaggctg
     4561 ttccgtccgc cacgagcgcg actcgcgccg ccagcaccac cacgccgccg ccgcagccca
     4621 cgcccacgat gtggagtagg cgaacgccgc gctcgacggg ctctccgaga cgcgccaccc
     4681 gacgagcatg aggacaatcc acacgagcgg ctgtcccgcc cagaagacgt agagcgtgta
     4741 acgcatgatc ttgaggccgc gcgtcgccgc gagccaaacg ttcgcaaggg ccaggaacgg
     4801 aagcccggcg gccgcggcct gaatcgcacc gcgcccccgg atggggtcga gagacagcag
     4861 cgaccggaga gcatcggctc ccgcgaccgc agcaagcgca accacagtgc tcgccgcggc
     4921 cgcgatgagg gcggcgcgcg ccacgaccgt gcggagcgcg ttcgtccgcc cctcgccggt
     4981 ggcggttgcg acggttcgca acaccgccat atccattcct gcacgggtga gaaacgacgt
     5041 gacgaatgcc gcttgggtca cgaccgtcac cagcccgaat cctgcgttgc cgagagtccg
     5101 gccgatcagg atttgcgcaa cgagcgtccc gagcgcggcg acgccgaccc caaccacatt
     5161 ctgtgccgtg cccgagagta tctggatcga tgcggagccg gaccccgaaa gcttgtctgc
     5221 tattcccatt cgatggtgcc gggcggcttc gacgtgatgt cgtagacgac acggtttacg
     5281 ccggggacct cgttcacgac ccggctcgaa acgcgctcga gaaggtcgta ctcgaggcgc
     5341 gcccagtcgg cggtcatcgc gtcgtcgctc gtgaccgcgc ggaggacgat cggatacccg
     5401 tagg
//
LOCUS       DATLFO010000003         5515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_9945, whole genome shotgun sequence.
ACCESSION   DATLFO010000003 DATLFO010000000
VERSION     DATLFO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9945"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VM841_00045"
     CDS             <1..1270
                     /locus_tag="VM841_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88600.1"
                     /translation="TDADYDGFLARLDESGVPIWARQIASPNAAFVRHVVVADDWVLA
                     TGYAGRFPEGSGREPISLDLGGGPITGNGGFDAFFAVYDRADGSYRSGRVIGGAGDEQ
                     AIQVVSLPGGEAAILGQFTETFEGRTARERDLFVLRLRADGSMRWIATAGGPADDEPG
                     GLAALPDGDLVVTGTFSHAFSIGDVELTGGRSAGFFARLSGDDGTVRWARGVGETDWS
                     KGAFIASESTDEVVVAAEFAETIRIGDASVAAQGQYDALILALDPGTGESRWFCRVGG
                     SDDEHPLGLAQSKDSLVVVGAAERPDRTADSVTAGDRRRGMALAVSPRTCTSKWTWST
                     AGDDDWVRRVVALETGTVALAGWHSRVGGTVAAGGRDIFVAVGRPPPGMTPAGRARGR
                     SGWVPVIVLTAGAALLGWSAAAFAKRRRFR"
     gene            1519..4671
                     /locus_tag="VM841_00050"
     CDS             1519..4671
                     /locus_tag="VM841_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HVL88601.1"
                     /translation="MAAGLAVGLAAIDAAPAAAAITTPPASSTQTGDLYAFEDRGATH
                     NSLCSEGRGRIELYSPSNVLIAQQFKGADRASGPFSVVFDTAGLASGTYRLRSVTQDR
                     PVSFVSCSDSSQVTRSDFTFTLSNGSTIRNAGATTGTTGESPTVSARLTRPGLHGRWW
                     ATGSPSTINEGATPSASRIHPTVDMTNYSIPAGGSNGRYENYNAVLTGYIVPAYSQAY
                     TINITTDDGVRVWVNNTQIINSWVSQGPTEYNGTTPVLQAGSLVPIRIEHFQGGGGQR
                     LRLRWSSASQGLEVIPASRLRNDNGIAGKPVTFELKDDDDIVYATAFATTNSSGVASA
                     ALALSAAPGSYDLHATYDGDAVYTGSTSSVPFTVTKRPVQVTNLTANASTLWDENQDV
                     SFRLTDGLSGAPLSGQDVSVSLGSSAETLATDSEGEVSTTFHLSDTPGSYQLSASFAG
                     NGTYLSGATSTPFTISRRPTALTYAGDTEGFWNENAAVSATLADALRGTPISGRSIDF
                     QIGSQSGSATSDDDGRAATSILLADDAGARVTSAYFGGDAFYTGTSDARDFTIKHRPT
                     TLAYVGDRVGLWNDSVTLSAKVTDDRLGDQGVAGLTVDFTLGSATTSAVTDAHGVASA
                     SVHLTDTPGAFTVTASFSGDGRYVASGESADFDINKRPTTVTYDGALNGRHNESATLS
                     ATLTDGSSGDAVAGKSVGFTLGSFSGSDDTDAAGAASVVAPMDQDAGTYTVSAAFDGD
                     DYYVAASDNATFTIGWEHTFTDDDGAGSVFINTSNKQFGFARADGYYSGVIDDPEMKI
                     LSVPSGIDPPSAPEPPDVPELPEIPQVPECLDDPLGGDPVCPADYLPPLPSAASSATA
                     STNECVDDPLGGDPICPPPAPDAPCDPNAPDGCIPETPEPPTAPCDPNDPASCLPAAP
                     TLCDLPGAYECHERFVLISYHSDELTLAGFFDLDTGQFIAVGRVGTEPFLLRNVIVPA
                     PPCNPEDPASCLPDVPETPEPPCDANDPEGCLPPAPEPPCDPNSPEGCVPETPEVPEP
                     PSAPQIPECLENPAGDPVCP"
     gene            4682..>5515
                     /locus_tag="VM841_00055"
     CDS             4682..>5515
                     /locus_tag="VM841_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88602.1"
                     /translation="MTTTTANRAPRPGLRAGGAVTGALLVALVLAALPARAETPAYAF
                     GVVTKTNPPASMRPGQPYPITVVLRNDGTETWFSEEAADGAHPVRLGTVEPDDRQSAF
                     AVQDAAWLWSKGRRVRMLTAVVPPGRNGVFAFTFTAPVLPGVYPEERFRPLAEGRAWM
                     TGSTIVFPPVTVPNPGAAPSVPAAGLPPEPCLPSMPRHEGARAPAVQDAGPPAAALGY
                     GAGSARGPALTGPTAVALAPNGDVFVADAANHRIVKMRPDGTVLKTFGSGQMLAPSGI
                     AV"
BASE COUNT          872 a         1941 c         1849 g          853 t
ORIGIN      
        1 gaccgatgcc gactacgacg gctttctggc ccgcctcgac gaatccggcg tgccgatctg
       61 ggcgcggcag atcgcctccc cgaatgcagc tttcgttcgt cacgtcgtcg tcgccgacga
      121 ctgggtgctt gcgaccggct acgcaggccg ctttccggaa gggtcgggtc gcgagccgat
      181 ctcgttggat ctcggcggag ggccgatcac cggcaacggc ggcttcgatg cgttcttcgc
      241 cgtctacgat cgagcggatg gatcctaccg atcgggccga gtcatcggtg gcgcaggcga
      301 cgaacaggcg attcaggtcg tttcgctgcc cggaggcgag gccgcgattc tcggtcagtt
      361 caccgaaacc ttcgaaggac ggaccgctcg cgaacgggac ctgttcgtcc ttcgccttcg
      421 tgccgacggc tccatgcgat ggatcgcgac cgcgggaggc cccgccgacg acgagccggg
      481 tggacttgcc gcgttgccgg acggcgatct ggtcgtgacg ggaacgttca gtcatgcttt
      541 ctcgatcggg gatgtcgagc taaccggcgg acggagcgcc gggttcttcg cgcgactgag
      601 cggcgacgac ggcaccgtcc gctgggcgcg cggcgtcggg gaaaccgact ggtcgaaggg
      661 agccttcatc gcatcggaat caacggacga ggtcgtcgtc gccgccgagt tcgcggagac
      721 gattcggatc ggggacgcct cggtagccgc acagggccag tacgacgcgt tgatcctggc
      781 gctcgatccc ggaacgggcg aaagccggtg gttctgccgg gtcggcggga gcgacgacga
      841 gcatcccctc ggcctggcgc aatcaaagga ctcactggtc gtggtcgggg ccgccgagcg
      901 acccgatcgc acagcggatt cagtgaccgc cggcgaccgc cgccgaggga tggcactggc
      961 cgtttcgcct cgaacctgca cgagcaagtg gacgtggtca acggcaggcg acgacgactg
     1021 ggtacgtcgc gtcgtcgctt tggagacggg aacggttgcc ctggcggggt ggcactcgcg
     1081 ggtgggcggc acggtcgctg cgggcggccg ggacatcttc gttgccgtcg gacggccgcc
     1141 gcctggaatg actcccgccg gacgcgcacg cgggcggagc ggatgggtgc ccgtcatcgt
     1201 cttgaccgca ggggcggcgc tgctgggctg gtccgcggct gcattcgcga agcgtcggcg
     1261 cttccgttag aaccgcccgg ccacgtcccc gaagcccggc ggagcagaaa aaccttgcgc
     1321 caacctttaa gactgccctg ccgtggacag agtgtcgata ccgctgcacc catttgtgac
     1381 atgtcgtccg tatcccaacc ggcaggcgca aacctacgat ggccggagtc gtacgcgcag
     1441 tatggaaaac ggggggattc catggctcgg ctcttgttgc gttcccggcg gtcgatcacc
     1501 gcggggcttc tgacactaat ggcggcgggg cttgcggtcg ggctggccgc gatcgacgcg
     1561 gctccggcgg cggcggccat caccacaccg ccggcgtcgt cgacccaaac cggcgacctg
     1621 tacgcgttcg aggatcgcgg agcgacccac aattcactct gttccgaggg ccgagggcgc
     1681 atcgagctgt acagcccgag caacgtcctg atcgcccagc agttcaaagg agcggatcgc
     1741 gccagcggac cgttcagcgt cgtgttcgat acggcgggtc ttgcgtcggg aacctatcgg
     1801 ctgcgaagcg tcacgcagga ccggccggtc tcgttcgtaa gctgctcgga ctccagtcag
     1861 gtcacgcgat ccgacttcac gttcacgctg tcgaacggct cgacgatccg caacgccggt
     1921 gccacgaccg gcacgacggg cgagagcccc acggtctccg cccgcttgac gcgtcccggg
     1981 cttcacggcc ggtggtgggc cacaggctcg ccgagcacga tcaacgaagg cgcgacgccc
     2041 tcggcctcgc gcatccaccc gaccgtcgac atgaccaact actcgatccc cgccggcggg
     2101 agcaacggac gctacgaaaa ctacaacgcg gtgttgaccg gctacatcgt gcccgcgtac
     2161 agccaggcgt acacgatcaa catcacgacc gacgacgggg ttcgcgtttg ggtcaacaac
     2221 acgcagatca tcaactcgtg ggtaagccag ggccccaccg agtacaacgg cacgacgccc
     2281 gtgctgcagg ccggctcgct cgttccgatt cggatcgagc acttccaagg cggcggcggc
     2341 cagcgccttc gcctgcgctg gtcctcggca tcgcagggcc tcgaggtcat cccggcgtcg
     2401 cggcttcgca acgacaacgg catcgccggc aagccggtca cgttcgagct gaaggacgac
     2461 gacgacatcg tctacgcgac ggcgttcgcg acgaccaact cctcgggcgt tgcgtcggcg
     2521 gctttggctc tctcggccgc gccgggatcc tacgatctgc atgcgaccta cgacggggat
     2581 gccgtctaca ccggctcgac cagcagcgtg ccgttcaccg tcacgaagcg gccggtccag
     2641 gtcacgaacc tgaccgcgaa cgcctcgacc ttgtgggacg agaaccaaga cgtgtccttc
     2701 cgcttgaccg acggtctctc cggagcgccg ctgtccgggc aggatgtttc ggtgtccctc
     2761 ggctcttccg ctgagacgct cgccaccgat tccgagggcg aggtctcgac gacctttcac
     2821 ctgtcggaca ctccgggctc atatcagctg agtgcgtcct tcgccggcaa cggcacctac
     2881 ctgtccggcg cgacgtccac gcccttcacc atcagccggc gacccactgc gttgacctac
     2941 gcgggggaca cggaaggatt ctggaacgaa aacgccgccg tctccgcgac gctggccgac
     3001 gcccttcgcg gcacaccgat ctcgggccga agcatcgact tccagatcgg gtcgcagagc
     3061 ggctcggcga catcagacga cgacggtcgc gccgcgacct ccatcctgct cgccgacgat
     3121 gccggtgcgc gtgtcacgtc agcgtacttc ggcggcgacg ccttttacac aggcacgagc
     3181 gatgcgagag acttcacgat caagcatcgg cccaccacgc tggcctacgt cggtgacaga
     3241 gtcggtctgt ggaacgacag tgtcacgctt tcggccaagg tcactgacga cagactcggc
     3301 gatcaaggcg tggcaggcct gaccgtggac ttcaccctgg gctccgcgac gacgagcgcc
     3361 gtgaccgacg cgcacggcgt ggcgagcgcg tcggtgcatc tgaccgacac gccgggggcg
     3421 ttcacggtga ctgcctcctt ctcgggagac gggcgttacg tcgcctcggg cgagtcagcg
     3481 gacttcgaca tcaacaagcg gccgacgacg gtcacctacg acggagccct caacggccgc
     3541 cacaacgaga gcgccacgct ttcggcgacc ttgacggacg gttcgagcgg cgatgccgtc
     3601 gcgggcaagt cggtcgggtt cacgctgggc tcattctcgg gtagcgacga caccgacgcc
     3661 gccggcgccg cttcggtcgt cgcgccgatg gatcaagatg cgggcaccta caccgtgagc
     3721 gccgccttcg acggcgacga ctattacgtg gccgcatcgg ataatgcgac gttcacgatc
     3781 ggctgggagc acaccttcac cgacgacgac ggtgccggat cggtcttcat caacacctcc
     3841 aacaagcagt tcggattcgc gcgcgcggac ggctactaca gcggcgtgat cgacgatccc
     3901 gagatgaaga tcctgtcggt gccgtcggga atcgacccgc cgtcggcgcc cgagcctccg
     3961 gacgttcccg agctgccgga gatcccacag gtgcccgagt gtctcgacga cccgctcggt
     4021 ggcgacccgg tctgcccggc cgactatctg ccgccgctgc cgtcggccgc gagctccgcc
     4081 accgcgtcca cgaacgagtg cgtcgacgat ccgctgggcg gcgatccgat ctgcccgccg
     4141 ccggcgcccg acgcaccctg cgacccgaac gcgccggacg gctgcatacc cgagacgccc
     4201 gagccgccca ccgcgccgtg cgacccgaac gacccggcct cgtgcctgcc ggcggctccg
     4261 acgttgtgcg atctgccggg cgcgtacgaa tgccacgaac gcttcgtcct gatcagctac
     4321 cactcggacg agctgacgct ggccggcttc ttcgatctcg acaccgggca gttcatcgcc
     4381 gttggcaggg tcggtaccga gccgttcctg ctccggaacg tgatcgtgcc ggcgccgccg
     4441 tgcaaccccg aggatccggc gtcgtgtctg ccggacgtgc cggagacgcc ggagccgccc
     4501 tgcgatgcca acgacccgga ggggtgcctg ccacccgcgc cggagccgcc gtgcgacccg
     4561 aactctccgg agggttgcgt ccccgagacg cccgaggtgc cggagccgcc gtcggcgccg
     4621 cagatccccg agtgcctgga gaaccctgcg ggcgacccgg tctgtccgta ggcggaaacc
     4681 gatgacgact acgaccgcga accgcgcccc tcgccccgga ctcagggcgg gaggcgcggt
     4741 cacgggcgcc ttgctcgtcg cgctcgtctt ggcggcgctg ccggcacgtg ccgagacgcc
     4801 cgcgtacgcc ttcggcgtgg tgacgaagac gaatcctccc gcgagcatgc gccccggcca
     4861 gccgtacccg atcacggtcg tgctgcgaaa cgacgggacg gaaacctggt tctccgagga
     4921 ggcggccgac ggcgcgcatc ccgttcggct cggcaccgtc gagcccgacg accggcagag
     4981 cgcgttcgcg gttcaggatg cggcatggct gtggtcgaag ggccgccgcg ttcgcatgct
     5041 gaccgcggtc gtgcctcccg ggcgaaacgg tgtgttcgcc ttcaccttca cggcgccggt
     5101 gcttcccggc gtctatcccg aagagcgctt ccggcctctc gcagagggcc gcgcgtggat
     5161 gaccgggagc acgatcgtct tcccgcctgt cacggtcccc aacccgggcg cggctccgtc
     5221 ggttccggct gccggcctgc cgccggaacc gtgcctgccg tcgatgcccc gccacgaagg
     5281 agctcgcgcc cccgcggttc aggacgccgg cccacccgcg gctgcgctgg gctacggcgc
     5341 gggatcggcg cgcggacccg cgctgaccgg ccccaccgcc gtcgccctcg ccccgaacgg
     5401 cgacgtcttc gtcgccgacg cggccaacca tcgcatcgtg aagatgcgtc ccgacggaac
     5461 ggtgttgaag acgttcggca gcggacagat gctcgcgccg agcgggatcg ccgtc
//
LOCUS       DATLFO010000004         4022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_119052, whole genome shotgun sequence.
ACCESSION   DATLFO010000004 DATLFO010000000
VERSION     DATLFO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119052"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VM841_00060"
     CDS             complement(<1..142)
                     /locus_tag="VM841_00060"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HVL88603.1"
                     /translation="MLRFLTAGESHGPALVATLEGMVSGVPVSGKMIRAELARRRHGY
                     GRG"
     gene            complement(293..853)
                     /locus_tag="VM841_00065"
     CDS             complement(293..853)
                     /locus_tag="VM841_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88604.1"
                     /translation="MAIDMKIGPRERALLIILGVVVALALVFVLFLRGGDETPQAGEP
                     FVPGRGTPVPTVSPAPTMPPETTEVFEGKDPFKPLIEAGAPGGGTGNGDGGNGGFQPG
                     PGTGTPTPGPTGGTRTGTRVELMDIFMQEGTRFASVKVGGEIHHVKEGDTFAGNYRVV
                     SLTDECGTFVLGDERFTLCIGQEVFK"
     gene            complement(856..1551)
                     /gene="pilO"
                     /locus_tag="VM841_00070"
     CDS             complement(856..1551)
                     /gene="pilO"
                     /locus_tag="VM841_00070"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HVL88605.1"
                     /translation="MRRQIAIAGAGLVVVLALVFVFLIRPVSSQIAEVKEQTETARSQ
                     ESSLRVQLRALQQARANATEVAARLAKFDLLLPRTSDIPAFIRQVQDAANLSGITLSS
                     IAPSPPSALAAGPGVDPVIAGKGVFGINVVLQVAGGFFRLESFLQRLENLQRVVMVNN
                     MSISPTVETDTGLFTLNSTITLQMFVVNPNLTTGGVPPSPAPTASPAPSPTGTPTGQG
                     GAGGPATPAPTPS"
     gene            complement(1548..2171)
                     /locus_tag="VM841_00075"
     CDS             complement(1548..2171)
                     /locus_tag="VM841_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88606.1"
                     /translation="MRRINLLPPELAAKRRSRQIASMIVIAGMVLLGLMFVIYGAQRA
                     RLASARGELERQQRVNAGLQRQVTQLSQFAQQQEQVRTKEQLLDALIKNEVRWSQVLT
                     EISLVIPADDWLTNFSGLVQQGIVGPGQPIGTIQVSGCTLLPADGTHLNVAQFLVQLG
                     RPLIFADDPFLTLSSIGSDTCPVQFSAQLQLTEQSRRGAQPGGGRRI"
     gene            complement(2172..3233)
                     /gene="pilM"
                     /locus_tag="VM841_00080"
     CDS             complement(2172..3233)
                     /gene="pilM"
                     /locus_tag="VM841_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HVL88607.1"
                     /translation="MPKGAVGLDIGTSAVRAAEIAGRKEPAILTRFAQIALPAGAVVD
                     GEIADPEAVASKIKELWRRGGFRMKRVAIGVANQKVVVRQIDLPFMEESELRGALQFQ
                     AQEYIPIPIEEAILDFQILEEFPGEGGERMMRVMLVAAQRDMINTFVSVVQQAGLDPV
                     LIDVTPFAALRPLIDRAPAVMEEREGEAVVDVGGGVTTIVVHEGGVPRFVRTLPSGGS
                     DITKALVAALGIPDEDAEGVKARVGLAPDGSQTPVEGAGKIIEERATAFMDEIRGSLD
                     YYQGQPGSAHVARVKLTGGGARLPNLAQRLSGVLHLPVEEAQPLAHVKLGKLGLTPEQ
                     LVQMSAVAAVAVGLSMGDT"
     gene            complement(3240..3452)
                     /locus_tag="VM841_00085"
     CDS             complement(3240..3452)
                     /locus_tag="VM841_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL88608.1"
                     /translation="MTSRRGSYAGDRLLTVGEVAAVMRVSNMTVYRLIKNGQLGAIRV
                     GKNYRIRESDVNRYLSDRAVRFDPEE"
     gene            complement(3689..>4022)
                     /locus_tag="VM841_00090"
     CDS             complement(3689..>4022)
                     /locus_tag="VM841_00090"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HVL88609.1"
                     /translation="PGWGAFARAAGGAAASFAVFNVIHFISPGGMGYGDVRLSALIGL
                     YLGYLGWGRVYAGFLLGFLLGAVIGLVLMMAGKAGRKTAVPFGPFLALGAVISAFWGQ
                     AIVDLWTP"
BASE COUNT          659 a         1354 c         1371 g          638 t
ORIGIN      
        1 tgccgcggcc gtagccgtga cgccgccgcg cgagctcggc gcggatcatc ttgccggaca
       61 cgggcacgcc ggagaccatg ccttcgagcg tcgcgacgag cgcgggcccg tgggattcac
      121 cggcggtcag gaatcgcagc atgatggcaa tcttaccggc cgctcggggc gccgtttcgc
      181 acgccgcgaa cgcgcgggga aaagaggtgg ggaacggcga tgagccaccg ccgttccccg
      241 gtgttgttcg tccgccgctg ctccccctga gtcacgacgg acgaacgctt tgctacttga
      301 agacctcctg gccgatgcac agggtaaacc gctcgtcgcc gagcacgaac gtgccgcact
      361 cgtcggtcag ggacaccacg cgatagttgc ccgcgaaggt gtctccctct ttgacgtggt
      421 gaatctcccc gcccaccttg accgaggcga atctggttcc ctcctgcatg aagatgtcca
      481 tcagctccac gcgggttccg gtgcgtgttc cgccggtcgg cccgggcgtc ggcgtcccgg
      541 tgccgggtcc cggctggaag ccgccgttgc caccgtcgcc gttgccggtc ccgccgcccg
      601 gagcgcccgc ttcgatcagc ggtttgaacg ggtccttgcc ctcgaacacc tcggtcgttt
      661 cgggcggcat ggtgggtgcc ggggacacgg tcggaacggg cgtgccgcgg cccgggacaa
      721 acggttcgcc ggcctgcggc gtctcgtcac caccgcgcag gaacagcacg aagacgagcg
      781 ccaaggcgac gaccacgccc aggatgatga gcagggcgcg ctcgcggggt ccgatcttca
      841 tgtcgatggc catggctagc tgggggtcgg agccggggtg gcggggccgc ctgcgccacc
      901 ctggccggtc ggagtccccg tcggcgatgg ggccggggac gcggtcggag ccggcgacgg
      961 cgggacgccg ccggtggtga ggttcgggtt caccacgaac atctggagcg tgatggtcga
     1021 gttgagcgtg aacaagccgg tatcggtctc gacggtcggc gagatggaca tgttgttgac
     1081 catgacgacg cgctgcaggt tctcgagccg ttgcagaaag ctttcgagac ggaagaaccc
     1141 gccggcgacc tgcagcacca cgttgatgcc gaacacgccc tttccggcga taaccggatc
     1201 cacgccggga ccggccgcga gcgccgaggg cggggacggc gcgatcgacg acagcgtgat
     1261 accggaaagg ttcgccgcgt cctgcacctg gcggatgaag gccgggatgt cgctcgtgcg
     1321 cggcagcagc aggtcgaact tggcaaggcg cgccgcgacc tcggtcgcgt tcgcgcgggc
     1381 ctgctgcagg gcgcgaagct ggacgcgcag gctgctctct tgcgagcgcg cggtctcggt
     1441 ctgctccttg acctcggcga tctgcgagga gacgggacgg atcaggaaca cgaacacgag
     1501 cgccaagacc acgacgagcc cggcgccggc gatagcgatc tgacgtctca tatgcgccgt
     1561 ccgcctcccg gttgggcgcc gcggcgcgac tgctcggtga gctgcagctg cgcgctgaac
     1621 tgcaccgggc aggtgtcgga gccgatcgaa gacagcgtca ggaacggatc gtcggcgaag
     1681 atgagcggcc ggccgagctg cacgaggaac tgcgccacgt tcaggtgcgt cccgtccgcc
     1741 ggcagcaagg tgcagcccga gacctggatg gtgccgatcg gctgcccggg tcccacgatt
     1801 ccctgctgga cgagaccgct gaagttggtc agccaatcgt cggccggaat cacgaggctg
     1861 atctcggtga gcacctggct ccaacgaacc tcattcttga tgagcgcgtc caggagctgc
     1921 tccttcgtcc gcacctgctc ttgctgctgc gcgaactgcg aaagctgcgt gacctgccgc
     1981 tgcaggcccg cgttgacgcg ctgctgacgc tcgagctcgc cgcgcgccga cgccaggcgc
     2041 gcacgctgcg cgccgtagat gacgaacatg aggccgagca ggaccatgcc cgcgatgacg
     2101 atcatgctcg cgatctgccg gctgcgccgc ttggcggcca gctcgggggg aaggaggttg
     2161 atgcgccgca tctaggtgtc ccccatcgac aatccgaccg cgaccgcggc gacggcggac
     2221 atctgcacga gctgctcggg cgtcaggccg agcttgccga gcttcacgtg cgcgaggggc
     2281 tgtgcctcct cgaccggaag atgcaacacc ccggacaggc gctgcgcgag gttgggcagc
     2341 cgcgctccgc cgccggtgag cttgacccgc gcgacgtgcg ccgatccggg ctggccctgg
     2401 tagtagtcga gagagccgcg aatctcgtcc atgaatgccg tggcgcgctc ttcgatgatc
     2461 ttgcccgcgc cctcaacggg cgtctgggag ccgtcgggtg cgaggcccac acgcgccttg
     2521 acaccctccg catcctcgtc cgggatgccg agggcggcga cgagcgcctt ggtgatgtcc
     2581 gagccgcccg agggaagcgt gcgcacaaag cgcggcacgc cgccttcgtg tacgacgatg
     2641 gtcgtgacgc cgcctccgac gtcgacgacg gcttcgcctt ccctctcctc catgacggcc
     2701 ggtgcgcgat cgatcaacgg ccgcagggcc gcaaaggggg tcacgtcgat gaggaccgga
     2761 tccaagcccg cctgctgaac gaccgacacg aaggtgttga tcatgtcgcg ctgggccgcg
     2821 accagcatca cgcgcatcat gcgctcgccg ccctcgccgg ggaactcctc gagaatctgg
     2881 aagtcgagga tcgcttcctc gatcgggatg ggaatgtatt cctgcgcctg gaactggagc
     2941 gcgcctcgca gctcgctttc ctccatgaac ggcaggtcga tctgacgaac gacgaccttt
     3001 tggttcgcca cgccgatcgc cacacgcttc atgcggaacc cgccgcggcg ccacagctcc
     3061 ttgatcttgc tcgcgacggc ctcggggtcg gcgatctccc cgtccacgac ggcgccggca
     3121 ggcagggcga tctgcgcgaa gcgggtcagg atcgcgggct ctttgcggcc ggcgatctcg
     3181 gcggcacgca cggcgctcgt gccgatgtcc agccccacgg ctcccttcgg catcccgaac
     3241 tactcctcgg ggtcgaagcg gacggctcgg tccgacaggt atcggttcac gtcggactcg
     3301 cggatccggt agttcttccc gacccggatc gcgccgagct ggccgttctt gatcagccgg
     3361 tacacggtca tgttggacac gcgcatgacc gccgccacct cgccgaccgt caagaggcgg
     3421 tcgccggcat acgaacctcg ccgggatgtc atggggacct cgatctctgt caccgggttc
     3481 tctaggggtc tgagccgctt tttcacacta gcaacatatt caacatccgc aacggaagcg
     3541 accgcatgtt ttcaggtggt tgctgttgtt gtcaagaggg gtcctcggcc tcgtttcgcc
     3601 acttcttgag aactacacac ctgtaactag cagtcgtggt caccaacgcc cgcgtagcgc
     3661 ggccatctgg acgcgacccg tagtcaagct agggggtcca gagatcgacg atggcctggc
     3721 cccaaaaggc cgagatgacg gccccgagag cgaggaaggg gccgaagggc accgcggtct
     3781 tgcggcctgc cttgccggcc atcatcagaa ccaacccgat cacggctccg agaaggaaac
     3841 cgagcagaaa tcccgcgtag acgcggcccc accccagata gccgaggtaa aggccgatga
     3901 gggcgctgag tcggacgtcg ccgtagccca tgcctcccgg cgatatgaag tggatcacgt
     3961 tgaagaccgc gaacgaggcc gccgctcccc cggcggcccg ggcgaaggca ccccagcccg
     4021 gt
//
LOCUS       DATLFO010000005         4735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_99223, whole genome shotgun sequence.
ACCESSION   DATLFO010000005 DATLFO010000000
VERSION     DATLFO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99223"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VM841_00095"
     CDS             <1..395
                     /locus_tag="VM841_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131143.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HVL88610.1"
                     /translation="LYPPKKFFGAARNIEEGGSLTILASALVETGSRMDEVIFEEFKG
                     TGNSEIRLDRALAEKRIYPAIDVEASGTRKEELLYEKEELMLVWRLRRVLHALSPSAA
                     MELLRDKISSTKSNEQFLKEINKVPVND"
     gene            617..880
                     /gene="rpmE"
                     /locus_tag="VM841_00100"
     CDS             617..880
                     /gene="rpmE"
                     /locus_tag="VM841_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HVL88611.1"
                     /translation="MKAGIHPEYVECHVKCSCGNEFTTRSTRNELHVELCSQCHPFYT
                     GKQKLVDVGGRVEKFQRRYGNKVVSKKAAKAAEGDSAPETPAE"
     gene            884..1879
                     /locus_tag="VM841_00105"
     CDS             884..1879
                     /locus_tag="VM841_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HVL88612.1"
                     /translation="MAKKKRDYFYGGQAVLEGVMMRGPRSWAIAVRRPSQEIYVEHNP
                     VKGLAAKSPIWSKPLFRGVAALGEAMTIGVRALSISANQSLEEEEQLTPRQMAFSLVL
                     AFVFFIGVFIVLPTIGVGFLRKRTQSSLVSNLIEGGVRLGIFVGYLAAISLIKDIRRV
                     FMYHGAEHKTIAAHEAGEPQLTPEAVDKYSTLHVRCGTNFLIMVMMLTIVIYSFLGYP
                     VWYWRIVQRVSLIPVIAGISYEGLRLGANHIDNIFVRALMKPGIWLQKITTKPPTPDQ
                     IEVAIRSFATVLPAEEAARIIPADLPSPIVDGGSSVQPGWTPEPLPAPNPDSPAS"
     gene            2160..3224
                     /gene="prfA"
                     /locus_tag="VM841_00110"
     CDS             2160..3224
                     /gene="prfA"
                     /locus_tag="VM841_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVL88613.1"
                     /translation="MLDKLAAIEARFSEIERQLSDSAVASDPDKLRTLGKEHSELEPI
                     VAASREYRGALDDLAAAKEMLRDSSADPYLRAEVDQTEERIEKLEQQLKDLLVPKDPN
                     DARDVIVEIRAAAGGDEAGLFARDLYEMYSRYAEGRRWKIEMLSGSESDRGGFKEVSF
                     AVKGKGAWSRLKHESGVHRVQRVPATESQGRIHTSTATVAVLPEAEDVDIDINPSDLK
                     IDVYRSSGPGGQSVNTTDSAVRITHVPTGEVVACQEERSQLQNKERAMRLLRARLLAR
                     AIEEARAEYEASRKAAVGTGDRSEKIRTYNFPQNRVTDHRIGLSLQKLPQVMAGDIDE
                     LVDALIAKDRADRLESNGDD"
     gene            3241..4095
                     /gene="prmC"
                     /locus_tag="VM841_00115"
     CDS             3241..4095
                     /gene="prmC"
                     /locus_tag="VM841_00115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVL88614.1"
                     /translation="MAELLARAETMLAAAGVDTPKGDAQWIAAHVLGVNRTSLLAHPE
                     RVVELETAEHLESLVARRAEREPLGYVLGTVNFRGLVMEVGPGVFCPRPETEVTAARA
                     IARARERGPRPCVVDVCAGSGPIALSVAAEVPNARVFATEISPAARGWALRNLARTGL
                     RVTLLPGDLFDPLHPALGGAVDVIVSNPPYIPQGAIESLPDEVRRYEPREALTGGADG
                     LDVVMRLIGDAPRWLTHGGWLVMEVGEEQAERIAKVLAAAGYDSVEIGPDLAGRERVV
                     EGRWVGNP"
     gene            4092..4733
                     /locus_tag="VM841_00120"
     CDS             4092..4733
                     /locus_tag="VM841_00120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVL88615.1"
                     /translation="MSELVSLLDERERGIARAAEVLRMGELVVAPTDTIYGLLGDAFK
                     PWATAKIFEIKQRPRSLPLPILVSRPKQAWALCGAVPDAALKLAAAFWPGALTMVLPE
                     NPELDWDLGERTGSIAVRMPAQEDLLEIIAMVGPVAGTSANPSGAATAPDVHGVREAL
                     GDQVALYLDGGESPADTGSTIVDLTGEQPKILREGPIPAQAVLDAAGDWKAAD"
BASE COUNT          801 a         1599 c         1635 g          700 t
ORIGIN      
        1 cgctgtaccc gccgaagaag tttttcggtg cggcgcgaaa catcgaagag ggcggctcgc
       61 tgacgatcct ggcctcggcc ctggtcgaga ccggctcgcg catggacgag gtcatcttcg
      121 aggagttcaa aggcaccgga aacagcgaga tccgcctgga ccgcgccctg gccgagaagc
      181 gcatctaccc ggccatcgac gtcgaggcct cgggcacccg aaaggaagag ctgctctacg
      241 agaaggaaga gctcatgctg gtgtggcgcc tgcgccgcgt cctgcacgcc ctgtcgccgt
      301 cggccgccat ggagctgctg cgcgacaaaa tcagttcgac caagtccaac gagcagttcc
      361 tcaaggagat caacaaggtc cccgttaacg actagcccgg tccggaatca acccggaatc
      421 ggaacaaaag gggctgcccc ggccctgccg gtacccagat cgcgggaatg ccggtaccat
      481 tcgggtcgtt gttcgcctgc ccggcgggct ccagccggga tcgagacaag ccgaaacgat
      541 tctgggaccg cctgcggccg cgcccacgcg ggcggccggc gagggtctcg tggcgtggag
      601 gagaagcgag gaagacatga aggccggtat ccaccccgaa tacgtcgaat gccacgtgaa
      661 gtgctcgtgc gggaacgagt tcaccactcg ctccacgcgc aacgagctgc acgtcgaact
      721 gtgctcgcag tgccacccct tctacaccgg caagcaaaag ctggttgacg tcggtggccg
      781 cgtcgagaag ttccagcgcc ggtacggcaa caaggtcgtg tccaaaaagg ccgccaaggc
      841 cgccgagggc gattccgccc ccgagacgcc ggccgagtag cgcgtggcga agaagaagcg
      901 cgactacttc tacgggggcc aggcggtcct cgaaggcgtg atgatgcgcg gcccgaggtc
      961 ttgggccatc gccgtccgcc gtccctcgca ggagatctac gtcgagcaca atcccgtcaa
     1021 gggactcgcc gccaagagcc cgatctggag caagccgctg ttccgcggcg ttgccgcgct
     1081 cggcgaggcg atgacgatcg gcgtgcgcgc gctgtcgatc agcgcgaacc agtcgctcga
     1141 ggaagaggaa cagctcacgc cgcggcagat ggcgttctcg ctggtgcttg ctttcgtgtt
     1201 cttcatcggc gtattcatcg tgctcccgac catcggcgtc ggattcctgc ggaagcgcac
     1261 gcagtcgtcg ctggtgtcga acctgatcga aggcggcgtg cggctgggca ttttcgtcgg
     1321 ctacctcgct gcgatcagcc tcatcaagga catccggcgc gtcttcatgt accacggggc
     1381 cgagcacaag acgatcgccg cgcacgaagc gggggagccc cagctgacgc ccgaggcggt
     1441 cgacaaatac tcgacgctgc acgtccgttg cggaacaaac ttcctgatca tggtgatgat
     1501 gctcacgatc gtcatctact cgtttttggg ctatccggtc tggtactggc ggatcgtgca
     1561 gcgcgtcagc ctgatcccgg tcatcgccgg tatctcctac gaggggctgc ggctgggagc
     1621 gaaccacatc gacaacatct tcgtgcgggc gttgatgaag ccggggatct ggctgcagaa
     1681 gatcaccacc aagccgccga cgccagacca gatcgaggtg gcgattcgct cgttcgctac
     1741 ggtgctgccc gccgaggagg ccgcgcggat catccccgcc gacctgccgt ccccgatcgt
     1801 ggacggcggc tccagcgtgc agcccgggtg gacgcccgag ccgctccccg cgcccaaccc
     1861 ggactcgccc gcgtcctagc cgaagcgccc catagcgcgg acggtctccg tcgctcgatc
     1921 ttgcaggcgt gccgctcgac cgcacagcgg agacggccga gctcttcgtc tttccatacg
     1981 ggcgccgctt ggttccacgc ggacgacgca caccaggcgt acgcgcgggc caccgcgagc
     2041 actgccggcg tgggcgtgcc ggacagcgcg ctctcgaccg tccgtcaaga cggcggcgtc
     2101 gcgctctgcc acgactgacg agaaccgggg gcacacggcg ttgctagact ctcggcctca
     2161 tgctggacaa actcgccgcg atcgaagcac gcttttcgga aatcgaacgt cagctttcgg
     2221 attcggccgt cgcgtcggac cctgacaagc tgcgcacgct cggcaaggag cacagcgagc
     2281 tcgagcccat cgtcgcggcc tcccgcgagt atcgcggggc gctcgacgat ctggccgcgg
     2341 cgaaggagat gctgcgggac tcctcggccg acccgtatct gcgggcggag gtggaccaga
     2401 ccgaggagcg gatcgagaag ctcgagcagc agctgaaaga tctgcttgtc cccaaagacc
     2461 ccaacgacgc gcgcgacgtc atcgtcgaga tccgtgccgc cgccggcggc gacgaggccg
     2521 ggttgtttgc ccgcgacctc tacgagatgt actcccgcta cgccgagggc cgccgctgga
     2581 agatcgagat gctctcgggc agcgagtccg accgcggtgg cttcaaggaa gtgtccttcg
     2641 cggtcaaggg gaagggggcc tggtcgcgcc tgaagcacga gtccggcgtg caccgcgtcc
     2701 agcgcgtgcc cgccaccgag tcgcagggca ggatccacac ctcgaccgcg accgtggccg
     2761 tgctgcccga agccgaggac gtcgatatcg acatcaaccc gagcgacctc aagatcgacg
     2821 tctaccgctc gagcggtccg ggcgggcaga gcgtcaacac caccgactcc gccgtgcgca
     2881 tcacgcacgt gccgaccgga gaggttgtgg cctgccagga ggagcgctcc cagctccaga
     2941 acaaggaacg cgcgatgcgg ctgctgcggg cacggctgct cgcacgcgcg atcgaggagg
     3001 cgcgcgccga gtacgaggcc agccgcaagg cggccgtcgg caccggcgat cgcagcgaga
     3061 agatccgcac gtacaacttt ccgcagaacc gcgtcaccga ccaccggatc ggcctgtcgc
     3121 tgcagaagct gccccaggtg atggccggag acatcgacga gctcgtcgat gcgctcatcg
     3181 ccaaggatcg cgccgaccgg ctcgaaagca atggagacga ctgaccgaag cgcgcaatcg
     3241 gtggccgagc tgctcgctcg cgccgagacg atgctggccg ccgccggcgt ggacacgccg
     3301 aaaggcgacg cgcaatggat cgctgcgcac gtgctcggcg tgaaccgaac ctcgctgctc
     3361 gctcatcccg agcgcgtcgt cgagctcgag acggccgagc atctggagtc gctcgtcgcg
     3421 cggcgggccg agcgcgagcc gctcggctac gtgctgggca ccgtcaactt tcgcggactg
     3481 gtgatggaag tcgggcccgg cgtgttttgc ccccggcccg agaccgaggt caccgcggcc
     3541 cgcgcgatcg cccgcgcccg cgagcgcgga ccgaggccgt gcgtggtgga cgtgtgcgcc
     3601 ggctcgggcc cgatcgcgtt gtcggtcgcc gccgaggttc cgaacgcgcg cgtgttcgcg
     3661 accgagatct cacccgctgc gcgcggctgg gcgcttcgca accttgcgcg caccggcctc
     3721 cgcgtgacgc tgctgcccgg cgacctgttc gatccgctgc atcccgccct cggcggcgcg
     3781 gtggacgtca tcgtgtcgaa cccgccctac attccccagg gagcgatcga gtcgttgccc
     3841 gacgaagtgc ggcgctacga gccgcgcgag gcgctcaccg gcggcgccga cggcctcgac
     3901 gtcgtcatgc ggctcatcgg ggacgcgccg cgctggctca cgcacggtgg ctggctcgtg
     3961 atggaggtcg gcgaagagca ggcggaacgc atcgccaagg tgctcgccgc tgccggctac
     4021 gactcggtcg agatcggccc cgacctggcg ggccgggagc gcgtcgtcga aggccgctgg
     4081 gtgggcaacc cgtgagcgag ctggtctcgt tgctcgacga gcgtgagcgc ggcatcgcac
     4141 gtgccgccga ggtgttgcgc atgggcgagc tggtcgtggc gcccaccgac acgatctacg
     4201 ggcttctggg tgatgcgttc aagccgtggg cgacggcgaa gatcttcgag atcaagcagc
     4261 ggccacggtc cctgccgctg ccgatcctgg tgagccggcc gaagcaggcg tgggccttgt
     4321 gcggcgcggt gcctgatgcg gcgctcaagc ttgcggcggc gttctggccg ggcgcgctca
     4381 cgatggttct gccggagaac cccgagctgg actgggacct gggcgagcgc accggctcga
     4441 tcgcggtgcg catgcctgcg caagaggatc tgctcgagat catcgcgatg gtcggtccgg
     4501 tggccggaac gagcgccaac ccgagcggcg cggcgaccgc gcccgacgtc cacggcgtgc
     4561 gcgaagcgct cggcgatcag gtcgcgctct acctcgacgg gggcgagagc ccggccgata
     4621 ccggatcgac gatcgtcgac ctgaccgggg agcagccgaa gattttgcga gaagggccga
     4681 tcccggcgca ggccgtgctc gacgcggccg gcgactggaa ggccgcggac tagga
//
LOCUS       DATLFO010000006         8547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_119080, whole genome shotgun sequence.
ACCESSION   DATLFO010000006 DATLFO010000000
VERSION     DATLFO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119080"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /gene="gcvPA"
                     /locus_tag="VM841_00125"
     CDS             complement(<1..1327)
                     /gene="gcvPA"
                     /locus_tag="VM841_00125"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HVL88616.1"
                     /translation="MEFAPHTDDDVAQMLAAIGKRSIDDLFAHLPKAARFTGSLSIPD
                     GLSEAALLRETGALAARNRNADDLVCFLGAGAYDHHIPSMVWPLLLRGEFLTSYTPYQ
                     PELSQGVLQALFEYQTMIAELTGLDVSNASLYDGGSALPEAVSMASGATRRDAVVLAG
                     AINPLYVQVLETAAVGPGIEIRRAGRTPAGTADPAEVERLAAGAAAVAVQYPNFFGCI
                     EDVPALAEIAHAAGAKLIVHADLTSLGVLEAPGNLGADIVVGEGQSLGNPLNFGGPYV
                     GVIACRRDEVRRLPGRLVGETVDRKGRRTFVLTLQAREQHIRREKATSNICTNQTLNA
                     LATAITLSWLGPAGLKEMGEQCLAKARYARERLCEVAGATPAFDAPHFKEFCVRLPAD
                     PVRVAAHAAGAKLIVHADLTSLGVLEAPGNLGADIVVGEGQSLGNPLNFG"
     gene            complement(1460..1933)
                     /locus_tag="VM841_00130"
     CDS             complement(1460..1933)
                     /locus_tag="VM841_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88617.1"
                     /translation="MRNSNPTQEQQPSKKRRRKARAGKPATAKAAATAAARRQGALPS
                     DRTEFAHASERQYARLLDFYEIKWDYEPRTFDVEWDADGNVVKQFSPDFYLPEFDTYI
                     EVTTMNQKLVTKKNRKVRRLRELYPDVKIKIFYQRDYLALLAKYGLARPEDTEES"
     gene            complement(1848..2354)
                     /locus_tag="VM841_00135"
     CDS             complement(1848..2354)
                     /locus_tag="VM841_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVL88618.1"
                     /translation="MANGETNGETVQGFRGPTVCKTVGITYRQLDYWARTGLVTPSVQ
                     DAKGSGSQRLYSFSDVVHLKIIKRLLDAGVSLQQTRKAIDYLTETEQQPLHESTLVSD
                     GTSIYLCKSSDEVIDLLNRGQGVFGIAVGRIYEEVQGSVAELKPDAGAATEQEEAQEG
                     PGRETGHG"
     gene            complement(2500..2973)
                     /locus_tag="VM841_00140"
     CDS             complement(2500..2973)
                     /locus_tag="VM841_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HVL88619.1"
                     /translation="MIELQLVGVRVELPTNQPIVLLREREGERFLPIWIGAVEATAIA
                     FALQGIVTQRPMTHDLMKNILESAEINIDRILITDLKEGTFFATIEMSRNGTKYEVSS
                     RPSDAIALAVRVSVPIFAAEEVLNEASIVIRDDEEQEVEKFREFLEQVNPEDFKG"
     gene            complement(3041..3634)
                     /gene="hpt"
                     /locus_tag="VM841_00145"
     CDS             complement(3041..3634)
                     /gene="hpt"
                     /locus_tag="VM841_00145"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HVL88620.1"
                     /translation="MSEAGIVLTAVQIRERIAAIGAEISRDYAGRSPILVGVLKGSLL
                     FMADLVRAIDLPVRVDFMSISSYGSGSSGVVRILKDLDLALEGEDVILVEDIIDTGLT
                     VTYLLANLKARNPESLHVVALLDKSVRRIIEVPIRYKGFDIPDEFVVGYGLDYEGRYR
                     NVSTIVGVSNLDVLADGPGPLQPYLPDGWVEPDASGE"
     gene            complement(3644..4381)
                     /locus_tag="VM841_00150"
     CDS             complement(3644..4381)
                     /locus_tag="VM841_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVL88621.1"
                     /translation="MKPGRPFMSIGEVIDALQPDYPDVTVSKIRFLESAGLIDPERTP
                     AGYRKFYQKDLERLRFILKLQRDSYLPLKVIGERLAEYDAGMAPPPAENGHATPAAPR
                     PEVEPEEDLAEQVAPLALSEMELANAANLEPSQVKALRDFGVICEHPGQGVGPVFDAD
                     DLIVARIAREFIRFGIEPRHLKMFRQFADREAMLLQQVVTPMLRHRSPEARKQATQSL
                     SDLSRLVKKLRHAFLRQNLRSFLGQGE"
     gene            complement(4378..4857)
                     /locus_tag="VM841_00155"
     CDS             complement(4378..4857)
                     /locus_tag="VM841_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVL88622.1"
                     /translation="MRVMYCTSCGSPNIDDARYCGRCGTALQQASDDTTISLAADDFH
                     DDEALPSLEGLQPDQAVLVIRRGMGAGSTFLIDKEVSVAGRSPESDLFLDDVTVSRRH
                     AEILRDGTRFVVKDAGSLNGTYVNRERVDVAELATGDELQIGKFKLVFLTAPGEGNA"
     gene            complement(5012..5398)
                     /gene="gcvH"
                     /locus_tag="VM841_00160"
     CDS             complement(5012..5398)
                     /gene="gcvH"
                     /locus_tag="VM841_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVL88623.1"
                     /translation="MYPEDLKYTKEHEWARHEGSAVRVGITFFAQEALGDIVYVDLPA
                     AGTKVTAGQPFGEVESTKSVSDIYSPVSGTIQERNAALEEQPELVNQEPYGDGWMVVI
                     ELESPGELDSMMNAADYQRLLDDTAL"
     gene            complement(5436..6176)
                     /locus_tag="VM841_00165"
     CDS             complement(5436..6176)
                     /locus_tag="VM841_00165"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HVL88624.1"
                     /translation="MKIEPETTEHRERPRPRVPAVPRPGQIALALAVALLGFLLATQF
                     RASEDLQGRLAGEREEDLALLLANLQQRSDALIEETVALRIELARAASSQDQERVLAE
                     NAARQLADLRMLLGLVKARGPGIEIVVADPEATVAADTLVDTVQELRDAGAEAIEING
                     VRVVAETSFSGSPGSIRVNRATSLRAPYQIRAIGHPQTMAEAMRIPGGVVDAVTARPA
                     ATIDITEHESMVIASLHPAPRFSYATPT"
     gene            complement(6185..6514)
                     /locus_tag="VM841_00170"
     CDS             complement(6185..6514)
                     /locus_tag="VM841_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HVL88625.1"
                     /translation="MLVLFSLAAGVGLGLVLQPTVPGFVAPYLPMAVMAALDSVFGGL
                     RARLEGVFSERVFVVSFLTNSVLAGVLVGVGERLGVDDLSIAVVVVFGVRIFGNLAAI
                     RRHLFHA"
     gene            complement(6529..7260)
                     /locus_tag="VM841_00175"
     CDS             complement(6529..7260)
                     /locus_tag="VM841_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HVL88626.1"
                     /translation="MTDPTPPVAPAPRRFRGGIAGIVFVLAFLLSVSWARQGARAPLV
                     EERRDRLAELVVQRQQRADAIERELIRLRARAESLARGKGPAAAFQDEIERLAVFAGT
                     APVSGPGVAVELADSPQAAQEGSPDFRIQDVDLQLIVNALWSAGAEAIAVNGQRIVST
                     SAIRSAGQAILVNYNVLTSPYRIEAIGDPEGIQVRFDESEIAERFRTWSQVYRLSIKV
                     RSATSITLPAYTGSVRPRHAEPVES"
     gene            complement(7273..7914)
                     /locus_tag="VM841_00180"
     CDS             complement(7273..7914)
                     /locus_tag="VM841_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVL88627.1"
                     /translation="MATPSPTPPPPHTGGGHNEPSDAILTVPNLITFLRLALIPVFMW
                     LAIGPEQVGAAVAVGFVAGASDFVDGRVARRYNQVSKLGIALDPLSDRFLIAAAAAIL
                     IIQDMAPLWVVAVVLARDALLLAAVPLLSARGVPRPAVSWFGKAGTFGIMWGLGMFLA
                     AHIVVPPARWLQIVAWLFVIPGMIFAYIAAGGYARTTIAALRANSTDARPRVQ"
     gene            7956..8034
                     /locus_tag="VM841_00185"
     tRNA            7956..8034
                     /locus_tag="VM841_00185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7992..7994,aa:Pro,seq:ggg)
     gene            complement(8041..>8547)
                     /gene="engA"
                     /locus_tag="VM841_00190"
     CDS             complement(8041..>8547)
                     /gene="engA"
                     /locus_tag="VM841_00190"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="synchronizes cellular events by interacting with
                     multiple targets with tandem G-domains; overexpression in
                     Escherichia coli suppresses rrmJ mutation; structural
                     analysis of the Thermotoga maritima ortholog shows
                     different nucleotide binding affinities in the two binding
                     domains; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated GTPase EngA"
                     /protein_id="HVL88628.1"
                     /translation="DQDQRIADRVIEAGRAAILVLNKWDLLDEEARERIEKDLERQLP
                     HLRWAPVVRTSALTKRGVARVMPAVELARASWEKRIPTAALNGWIRAAVEKIPLGSRG
                     TRPPRIKYATQVTVRPPTVVVFASDRLPDHATRALENSLRAAFGFEGSPIRVMVRVRK
                     RERKPAGS"
BASE COUNT         1356 a         2856 c         2963 g         1372 t
ORIGIN      
        1 cgccgaagtt gagcgggttg ccgagcgact gcccttcgcc gaccacgatg tccgcgccca
       61 ggttgcccgg cgcctccagc acgccgagcg aggtcaggtc cgcgtgcacg atcagcttgg
      121 cgccggcggc gtgcgcggcg acgcgaacgg gatccgccgg cagccgcacg cagaactcct
      181 tgaagtgagg cgcgtcgaac gcgggcgtcg cgccggcgac ctcgcacagc cgctcgcgcg
      241 cgtagcgcgc cttggccagg cactgctccc ccatctcctt cagcccggcc gggccgagcc
      301 acgacagcgt gatcgcggtc gcgagcgcgt tgagcgtctg gttggtgcag atgttgctcg
      361 tcgccttctc gcggcggatg tgctgctcgc gcgcctgcag cgtcagcacg aacgtgcgtc
      421 gtcccttgcg gtccacggtc tcgccgacga gccggccggg caggcggcga acctcgtcgc
      481 gccggcaggc gatcacgccg acgtaggggc cgccgaagtt gagcgggttg ccgagcgact
      541 gcccttcgcc gaccacgatg tccgcgccca ggttgcccgg cgcctccagc acgccgagcg
      601 aggtcaggtc cgcgtgcacg atcagcttgg cgccggcggc gtgcgcgatc tcggcgagcg
      661 cgggcacgtc ctcgatgcac ccgaagaagt tggggtactg aaccgccacc gcggcggccc
      721 cggccgccag acgttcgacc tcggccggat ccgcggttcc ggccggggtg cggccggcgc
      781 ggcggatctc gattcccggc ccgaccgccg cggtctcgag cacctgtacg tacagcgggt
      841 tgatcgcgcc cgcgagcacg accgcatcgc gccgtgtcgc gccggatgcc atcgacaccg
      901 cctcgggcag cgccgatccg ccgtcgtaga gcgaggcgtt ggacacgtcc agaccggtca
      961 gctcggcgat catcgtctga tactcgaaca gcgcctgcag caccccctgc gacagctccg
     1021 gctggtaggg cgtatacgag gtcaggaact cgccgcgaag cagcagcggc cacaccatcg
     1081 agggaatgtg atgatcgtag gcacccgcgc ccaggaagca gaccaggtcg tcggcgttgc
     1141 ggttgcgtgc ggccagcgcg ccggtctcgc gcagcaaggc cgcctccgac agtccgtcgg
     1201 ggatgctcag ggatccggtg aagcgagcgg ctttgggcag atgcgcgaac aggtcgtcga
     1261 tcgagcgctt gccgatcgcg gccagcatct gcgcgacgtc gtcgtcggta tgcggagcga
     1321 attccaagga cgtcctccct cccccgggcg atcgaggggc cgtacggatg gcgctgccgg
     1381 ggaaggcgtc accgatatta ctcggttccc ggcgaccgcc cgcggcgaaa ctccccgaaa
     1441 tcccggtgaa cggccggggt caagactctt cggtgtcctc ggggcgggcc agcccgtact
     1501 tggcgagcaa agcgagatag tcgcgctggt agaagatctt gatcttgacg tccgggtaga
     1561 gctcgcgcag ccggcgcacc ttgcggttct tcttggtcac cagcttctgg ttcatggtcg
     1621 taacctcgat gtaggtgtcg aactccggca ggtagaaatc cggactgaac tgcttgacga
     1681 cgttgccgtc ggcgtcccac tcgacgtcga aggtccgcgg ctcgtagtcc cacttgatct
     1741 cgtagaagtc gagcagccgc gcgtactgcc gttcgctggc gtgcgcgaac tcggtgcggt
     1801 cggagggaag cgcgccttgg cggcgcgcgg cggcggtggc ggccgcctta gccgtggccg
     1861 gtttcccggc ccgggccttc ctgcgcctct tcttgctcgg ttgctgctcc tgcgtcgggt
     1921 ttgagttccg caacgctccc ctgcacttcc tcgtagatgc gtccgaccgc gatgccgaaa
     1981 acgccttggc cccggttgag aagatcgatg acctcgtccg acgacttgca cagatagatc
     2041 gaggtgccgt cggagacgag ggtggactcg tgcagcggct gctgctcggt ctcggtgagg
     2101 tagtcgatgg ccttgcgggt ctgctgaagg ctgacgccgg cgtcgagcag ccgcttgatg
     2161 atcttgaggt ggaccacgtc gctgaaggag tacagccgct ggctgcccga tcccttggcg
     2221 tcctgaaccg acggggtcac caggccggtg cgcgcccagt agtcgagctg ccggtatgtg
     2281 atgcctaccg tcttgcagac cgtcggcccg cggaatccct ggaccgtctc gccgttcgtc
     2341 tcgccgttcg ccatcgtgcc ccccccgggc cacacaccgg taattacgcg gctgtcacgc
     2401 tacggccgat gggcgcgaac tgtcaatgct cgctcgaaga aagttcgagt ctcaacccgc
     2461 gcttgagagt gggaaaaccg caggtcagag agttcgaggt cagcctttga agtcttcggg
     2521 gttgacctgt tcgaggaact cgcggaactt ctcgacttct tgctcctcgt cgtcgcggat
     2581 cacgatcgag gcctcgttca ggacctcctc tgcagcgaag atcggcacgc tgacccggac
     2641 ggcgagggcg atcgcatccg aggggcggga tgagacctcg tatttggtgc cgttgcgcga
     2701 catctcgatc gtggcgaaga aggtgccttc cttcaggtcc gtgatcagga tgcgatcgat
     2761 gttgatctcg gcgctttcga gaatgttctt catcagatcg tgcgtcatcg gccgctgcgt
     2821 cacgattccc tgcagggcga acgcgatggc cgtcgcctcc accgcaccga tccagatcgg
     2881 cagaaagcgc tcgccctctc gctcgcgcag caggacgatc ggctggttgg tcggcagctc
     2941 gacccggacc cccaccagtt gaagctcgat cacgccgtca tccctcctgt ttggccggag
     3001 cctagcaccg gcgagcgcaa cgcaacaacg gcacggagca tcattccccg ctcgcatccg
     3061 gctcgaccca gccgtcggga agatacggct gcagcgggcc cggcccgtcg gcgagcacgt
     3121 ccaggttcga cacgcctacg atcgtcgaca cgttccggta tcggccttcg taatcgagtc
     3181 cgtagccgac gacgaactcg tcgggaatgt cgaagccctt gtaacggatc ggcacctcga
     3241 tgatccgccg caccgacttg tccagcaagg cgacgacgtg cagggactcc ggattgcggg
     3301 ccttgaggtt ggcgagcagg tacgtgacgg tcaggccggt gtcgatgatg tcctcgacca
     3361 ggattacgtc ttcgccttcc aaggccagat ccaggtcctt gaggatgcgc acgacccccg
     3421 acgagccgga gccgtacgag gagatgctca tgaagtccac gcgaaccgga aggtcgatgg
     3481 cgcgcaccag atcggccatg aacagaagcg agcctttgag cacacccacc aggatcggcg
     3541 agcggcccgc gtagtcgcgg ctgatctcgg ccccgatcgc ggcgatgcgc tcgcggatct
     3601 gaaccgccgt caggacgatg ccggcctcgc tcacgcggtc aggctactcc ccctggccga
     3661 ggaacgaccg gaggttctgc cgcaagaacg cgtggcgaag ctttttcacg aggcgcgaaa
     3721 gatctgacag cgactgcgtg gcttgcttgc gcgcctccgg cgagcggtga cgcagcatcg
     3781 gcgtgacgac ttgctgcagc agcatcgcct cgcgatcggc gaactgccgg aacatcttca
     3841 ggtgccgtgg ctcgatcccg aagcggatga actcgcgggc gatgcgggcc acgatcaagt
     3901 cgtcggcgtc gaagaccggc ccgacgccct ggcccgggtg ctcgcagatc acgccgaagt
     3961 cccgcagcgc tttgacctgg ctcggctcga ggttcgcggc gttggcgagc tccatctccg
     4021 acagtgcgag cggcgcgacc tgctcggcga gatcctcctc gggctcgacc tcgggacgcg
     4081 gcgcggccgg cgtcgcgtgt ccgttctccg caggcggcgg agccatgccc gcgtcatact
     4141 cggcgagccg ctccccgatc accttgagcg gaaggtagga gtcgcgctga agcttcaaga
     4201 tgaaccggag acgctccaga tccttctggt agaactttcg atatcccgcc ggggtgcgct
     4261 cgggatcgat cagtccggcc gattccagga agcggatctt cgagaccgtc acgtccgggt
     4321 agtccggctg caacgcgtcg atgacctcgc cgatgctcat gaacggacgg ccgggcttca
     4381 tgcgtttcct tcacccgggg ccgtgaggaa gaccagcttg aacttgccga tctgcagctc
     4441 gtcgccggtg gcgagctcgg caacgtccac gcgctcgcgg ttgacgtagg tgccgttgag
     4501 cgagcctgcg tccttgacga cgaaccgggt gccgtcgcgc aagatctcgg cgtggcggcg
     4561 ggaaaccgtc acgtcgtcga gaaacaggtc gctctcgggg ctgcggccgg cgaccgagac
     4621 ctctttatcg atcaggaagg tactgcccgc gcccatgccg cggcggataa ccagcacggc
     4681 ctggtccggc tgcagaccct cgagagaagg tagagcctcg tcgtcgtgga agtcgtcggc
     4741 cgccaggctg atggtggtgt cgtcggaggc ctgctggagg gccgtgccgc agcgcccgca
     4801 gtagcgggcg tcgtcgatat tcggggaacc gcagctcgtg caatacatga ccctcacccg
     4861 tctcgtgcgt ccgctgcccc gatgggcttc ggccggccgg gcccgtcgtc gaagcgtggg
     4921 agccggagaa accctgaaga tataccatag ggtagacggg aactccttcg aggagcgact
     4981 gagggatccc gccgggtcgg ggcggacccg gctagagcgc ggtgtcgtcg aggagccgct
     5041 ggtagtcggc ggcgttcatc atcgagtcga gctccccggg cgattcgagc tcgatcacga
     5101 ccatccaacc gtcaccgtag ggctcctggt tgaccagctc aggctgctcc tccagggccg
     5161 cgttgcgttc ctggatagtg cccgagaccg gcgaatagat gtcggatacc gacttggtgg
     5221 actcgacctc gccgaacggc tggccggccg tcaccttggt tcccgccgcc ggcaggtcga
     5281 cgtagacgat gtcgccgagc gcctcctggg cgaagaacgt gatgccgacg cggacggcac
     5341 tgccttcatg gcgcgcccat tcgtgctcct tcgtgtactt cagatcttcg ggatacatgt
     5401 gctcctccgg gccgccggga tgcccggccg gccgtctagg tcggcgtagc gtaggagaac
     5461 cgcggagccg gatgcaagga ggcgatgacc atcgactcgt gctcggtgat atcgatggtc
     5521 gccgcgggcc gggcggtgac cgcgtccacg accccgccgg ggatgcgcat cgcctcggcc
     5581 atcgtctggg gatggccgat cgcacggatc tggtagggcg ctctcagcga ggtggcgcgg
     5641 ttgacgcgaa tcgagccggg cgatcccgag aacgaggtct cggccaccac gcgcactccg
     5701 ttgatctcga tcgcctcggc gcccgcgtcg cgcagctctt ggacggtgtc cacgagcgtg
     5761 tcggcggcca ccgtggcttc cgggtccgcc accacgatct cgatgccggg gccccgcgcc
     5821 ttcaccaggc cgagcagcat gcgaaggtcg gcgagctgcc gggcggcgtt ctcggccaga
     5881 acacgctcct gatcctggga ggaggcggcg cgggcgagct cgatgcgcag cgcgacggtc
     5941 tcttcgatta gggcgtcgga tcgctgctgc aggttcgcga gcagcagcgc cagatcctcc
     6001 tcacgttccc ccgccagccg gccctgcaga tcctcgctcg cgcgaaactg cgtggcgagc
     6061 aggaagccga gcagcgcgac ggcgagcgcg agcgcgatct gacccggccg cgggacggcc
     6121 ggcacacgcg gacgcgggcg ttcgcggtgt tcggtcgtct cgggctcgat cttcatggtc
     6181 tcctctacgc gtggaacagg tggcggcgga tcgcggccaa gttgccgaag atgcggacgc
     6241 cgaacaccac cacgaccgcg atcgacaggt cgtccacgcc gagccgctcc cccaccccga
     6301 cgagcacgcc ggcgagaacc gagttggtta ggaacgacac gacgaagacg cgctcggaga
     6361 agacgccttc gagccgggcg cgcaagccgc cgaagacgct gtcgagcgcg gccatgaccg
     6421 ccatcggcag gtacggggcg acgaagccgg gcaccgtcgg ctgcagcacc aggcccaggc
     6481 cgacgcccgc cgcgagcgag aacagcacga gcagcattgt gtctctcctt acgattcgac
     6541 cggctcggcg tggcggggtc gcacgctgcc ggtgtaggcg ggcaaggtga tggacgtggc
     6601 ggagcggact ttgatcgaca agcggtagac ctgggaccac gttcggaacc gttccgcgat
     6661 ctcggactcg tcgaagcgca cctggattcc ctcggggtcg ccgatcgctt cgatgcggta
     6721 gggcgaggtc agcacgttgt agttgacgag gatggcctgt ccggccgagc ggatcgcgct
     6781 ggtggaaacg atgcgctggc cgttgaccgc gatcgcctcc gccccggcgg accagagcgc
     6841 gttgacgatc agctgaagat ccacgtcctg gatgcgaaag tccggcgatc cttcctgggc
     6901 ggcctgaggg ctgtcggcga gctcgaccgc gacgccggga ccgctcaccg gagcggtgcc
     6961 ggcgaagacg gcgagccgct cgatctcgtc ctggaacgcg gcggcgggcc cctttccgcg
     7021 cgcaagggat tcggcgcggg cacgcagccg gatgagctcg cgttcgattg cgtccgcgcg
     7081 ctgctgacgc tgcaccacga gttcggcgag ccggtcgcgg cgctcctcga cgagcggggc
     7141 gcgcgcgccc tgccgggccc aggagaccga cagaaggaag gcgagcacaa acacgatccc
     7201 ggcgataccg ccgcggaagc gacgcggcgc cggagcaacc ggtggggtcg ggtcggtcac
     7261 gttgcggtga gactactgca cgcgggggcg cgcgtccgtg ctgttcgcgc gcagcgccgc
     7321 gatcgtggtg cgcgcgtacc cgcccgcggc gatgtaggcg aagatcatgc cggggatgac
     7381 gaagagccac gccacgatct gaagccagcg agcgggcggg acgacgatgt gggcggccag
     7441 gaacatcccg aggccccaca tgatcccgaa cgtcccggcc ttgccgaacc atgagaccgc
     7501 cggccgcggc acgccgcgtg ccgacaggag cggcaccgcg gccagcagca gggcgtcccg
     7561 cgcgagcacc acggccacga cccagagcgg cgccatgtcc tggatgatca ggatcgcggc
     7621 cgcggcagcg atcaggaagc gatccgacaa cgggtcgagc gcgatgccga gcttcgagac
     7681 ctggttgtag cgccgcgcaa ccctgccgtc gacgaagtcg ctcgcgcccg ccacgaaacc
     7741 gacggcgacg gccgcaccca cttgctcggg cccgatcgcg agccacatga agaccggaat
     7801 cagcgcgagc cgcaggaacg tgatgaggtt cggaacggtg aggatcgcgt cggagggctc
     7861 gttgtggccg ccgcccgtgt gcggcggcgg cggtgtcgga gagggcgtgg ccacggcgcg
     7921 attctagtgg aggtcctata ctttgcaggc ttcgacgggc tgtggctcag tttggtctag
     7981 agcgctggac tgggggtcca gagaccggcg gttcaaatcc gcccagcccg accaagacga
     8041 tcagctgcct gcgggcttgc gctcgcgctt gcggacgcga accatcacgc ggatcggcga
     8101 gccttcgaac ccgaacgccg cgcgcaggct gttttcgagg gcgcgcgtcg cgtggtcggg
     8161 cagccggtcg ctggcgaaga ccacgaccgt cggcggccgc accgtcacct gcgtcgcgta
     8221 cttgatgcgc ggcggccgcg tgccccgcga tcccaacggg atcttctcga ccgcggctcg
     8281 gatccatccg ttgagcgcgg cggtggggat ccgcttctcc cacgacgcgc gcgcgagctc
     8341 gacggccggc atcacgcgcg ccacgccgcg cttcgtcagc gccgatgtgc gcacgaccgg
     8401 cgcccagcgc aggtgcggta gctgtcgctc gagatccttc tcgatccgct cgcgggcctc
     8461 ttcgtcgagc aggtcccatt tgttgagcac aaggatcgcc gcgcgtccgg cctcgatcac
     8521 gcggtcggcg atccgctggt cctggtc
//
LOCUS       DATLFO010000007         3734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_69696, whole genome shotgun sequence.
ACCESSION   DATLFO010000007 DATLFO010000000
VERSION     DATLFO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69696"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VM841_00195"
     CDS             <1..399
                     /locus_tag="VM841_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL88629.1"
                     /translation="TSDFHCVTGWSKLDNTWEGVSIHTVAEAAAIRPDATHVLALAPY
                     AYSANIPLEVLLDDDVLLAWSHDGAPLEPEHGGPLRLVVPKRYAWKSVKWIEGLQVLD
                     RDVRGYWEERGYHNNAEPFAEERYSWQESG"
     gene            complement(396..869)
                     /locus_tag="VM841_00200"
     CDS             complement(396..869)
                     /locus_tag="VM841_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HVL88630.1"
                     /translation="MEPALHPDCEPLAFLLGTWSGEGRGHYPTIKPFAYGEEVRFWHV
                     GKPFLAYSQRTWALDDSRPLHTEAGYWRPKPEGRIEIVLVHPTGITEIQEGTMDGGVI
                     AVRSTQIGLASTAKDVTALSRVFRVESGTLTYDLEMAAVGLPLQHHLNATLERTS"
     gene            907..1329
                     /gene="arfB"
                     /locus_tag="VM841_00205"
     CDS             907..1329
                     /gene="arfB"
                     /locus_tag="VM841_00205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HVL88631.1"
                     /translation="MRDLNIGRDLVIPSHEITQRFARAGGPGGQNVNKRDTKVDVLFD
                     LDGSESLSADQKRRVRRALKTRIDAAGVLRVSASAHRTQAQNRERALDRMEELLKEAL
                     APPPPPRVKTRPSRAAKERRIQDKKRRGEIKRLRRAAE"
     gene            complement(1339..1884)
                     /locus_tag="VM841_00210"
     CDS             complement(1339..1884)
                     /locus_tag="VM841_00210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HVL88632.1"
                     /translation="MLLFVVALALVGTACGSNIEQGSPDAGGGTNTPTEPPSQAPDAK
                     LVREQPADCKVEASADLAKKPEIKIPDCTPSTDLIAVDLVPGTGAEFKAGQEATIHYV
                     GISWSNKEQFDASWDRNEPFSTTIPGQLIDGWNEGIPGMKVGGRRLLIIPPSKGYGPS
                     GQGPIGPNETLVFVVDLKAAK"
     gene            2072..2836
                     /locus_tag="VM841_00215"
     CDS             2072..2836
                     /locus_tag="VM841_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HVL88633.1"
                     /translation="MNVTVCVKQIPDPNQTYELVDGRLKRDVAQSVLDPGDEFGVEAA
                     LKLTEASGGEVTVVSMGPSKAMEAVRRALSMGAAKGILVTDDALAGADALVTAKVLAA
                     AIKRNPFDLVICGVESTDGYSGVVPAMLAELLDVPQATFVKQLEVEGETLKIQRQTTL
                     GYDVVTCPLPAVMTVTAGVNEPRYPTFKGIMQAKSKPVEELSLSDLGLSPDDAKPTQT
                     VAAVNPAPERGQGEIVEDDGSGAAKIADFLKEAKVI"
     gene            2838..>3734
                     /locus_tag="VM841_00220"
     CDS             2838..>3734
                     /locus_tag="VM841_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HVL88634.1"
                     /translation="MAKIWVYAELDSHGNLEKTSLEILTKARTLGGEVEAVALGPGAN
                     AAAGPLGEHGATTVYACEDAVFTDFVGQPAAHVLYNLVQEHKPNLLLFPFTYDSRDVA
                     GRLAAKTGSTVMGSVTDVLSDTSAQTQIFGGSKLVDVTLEGPDPKVALIRPKAFEAEP
                     SGGSATTVAVTADVPDTAKKAKRVERIEEEQSGPKLEEAPVIVSGGRGLQDPSNFSLL
                     EELAGAIGNAAVGATRAVVDAGWVPYAMQIGQTGKTVKPKVYLAIGISGATQHIVGMK
                     SSDRIVAINKDAEAPIFQLADLG"
BASE COUNT          637 a         1285 c         1252 g          560 t
ORIGIN      
        1 acctccgatt tccactgcgt aaccggatgg tccaagctcg acaacacctg ggagggcgtg
       61 tcgatccaca ccgtcgccga ggcggctgcg atcaggccgg acgccaccca cgtgctcgcg
      121 ctcgcgccgt acgcctacag cgcgaacatc ccgctcgagg tgctcctcga cgacgacgtg
      181 ctgctggcct ggagccacga cggcgcgccg ctcgagcccg agcacggcgg cccgctccgc
      241 ctcgtcgtgc ccaagcgcta cgcgtggaag agcgtcaagt ggatcgaagg cctgcaggtc
      301 ctcgaccgcg acgtgcgcgg ctactgggag gaacgcggct accacaacaa cgccgagccc
      361 ttcgccgaag agcgctacag ctggcaggag agtggctagg acgtgcgctc gagcgtcgcg
      421 ttcaaatggt gctgcagcgg caggccgacg gccgccatct cgaggtcgta ggtgagcgtg
      481 ccgctctcga cgcggaagac gcgcgagagc gcggtgacgt ccttggccgt ggacgcgagc
      541 ccgatctgcg tggaccggac cgcgatcacg ccgccgtcca tggtgccttc ctggatctcg
      601 gtgatcccgg tcggatgcac cagcacgatc tcgatgcgcc cttcgggctt gggccgccaa
      661 tagccggcct cggtgtggag cggccggcta tcgtcgagcg cccacgtccg ttgcgagtac
      721 gcgaggaacg gcttcccgac gtgccagaag cggacctctt cgccgtaggc gaacggcttg
      781 atggtcgggt aatgaccgcg gccttccccg ctccacgttc ccaacaagaa cgccagtggt
      841 tcgcagtcgg gatgcagcgc aggttccatg gcagggcaac gctagcacgc tagccttgac
      901 ggggtcatgc gcgatctcaa catcggccgc gacctcgtga tcccctcgca cgagatcaca
      961 cagcgcttcg cgcgcgccgg cggccccggc ggccagaacg tcaacaagcg cgacaccaag
     1021 gtggacgtgc tcttcgacct cgacggctcg gagtccctca gcgccgatca gaagcgccgg
     1081 gtccgccgag cgctcaagac ccgcatcgac gccgcgggtg tccttcgcgt cagcgcgagc
     1141 gcgcaccgca cccaggcgca gaaccgcgaa cgtgcgctcg accgcatgga ggagcttctg
     1201 aaagaagcgc tcgcgcctcc gccgccgccg cgcgtcaaga cgcggccgtc gcgcgccgcg
     1261 aaggaacgcc ggatccagga caagaagcgg cgcggcgaga tcaaacgtct tcgccgcgcc
     1321 gccgaatgat cgatcgcgct acttggctgc cttcagatcc accacgaaca cgagcgtctc
     1381 gttcgggccg atcggaccct gaccgctggg gccgtagcct ttcgacggcg ggatgatcag
     1441 caggcggcgt ccgccgacct tcatcccggg gattccctcg ttccagccgt cgatcagctg
     1501 gccggggatg gtcgtggaga acggctcgtt gcgatcccac gacgcgtcaa actgctcctt
     1561 gttggaccag gagatgccga cgtagtggat ggtggcctcc tgaccggcct tgaactcggc
     1621 gccggttccc ggcaccagat ccaccgcgat cagatccgtg gacggcgtgc agtccgggat
     1681 cttgatctcg ggcttcttgg ccagatccgc ggatgcctcg accttgcagt ccgcgggctg
     1741 ctcgcggacg agcttcgcat ccggtgcctg cgagggcggc tccgtcgggg tattcgtgcc
     1801 gccacccgcg tccggcgagc cctgctcgat gttgctgccg cacgcggtgc ccaccagagc
     1861 gagcgcgacg acgaagagaa gcattcgacg caatggaact cccttccaga agaagcttcg
     1921 atatgaccgg cccgccatga gccacgagcc gggcgaaatg ataccggccg gttcgtggcc
     1981 gggcgcgcgg cccgggggtg cttgccactt gcgagcagtc gcttgcatcc gttttgcgcg
     2041 cgaccaagcc ccgcgatacg gtgccccggc catgaatgta accgtctgcg tcaaacagat
     2101 tcccgacccc aaccagacct acgagctggt ggatggtcgg ctcaagcgtg acgtcgccca
     2161 gtccgttctg gacccgggcg acgagttcgg cgtcgaagcg gccctgaagc tgaccgaagc
     2221 ctccggcggc gaggtcacgg ttgtttcgat gggtccgtcc aaggccatgg aagcggttcg
     2281 ccgcgcgctg tcgatgggag ccgccaaggg catcctggtc accgacgacg cgctcgccgg
     2341 cgcggatgcg ctcgtgacgg ccaaagtcct tgcggccgcg atcaagcgca acccgttcga
     2401 tctggtgatc tgcggcgtcg agtccaccga cggctactcg ggcgtggtgc ccgcgatgct
     2461 cgccgaactg ctcgacgtgc cgcaggccac gttcgtcaag cagctcgagg tcgagggcga
     2521 gaccctcaag atccagcgcc agaccacgct cggctacgac gtcgtcacct gtccgctgcc
     2581 cgcggtcatg acggtgaccg ccggcgtgaa cgagccgcgc tacccgacgt tcaagggcat
     2641 catgcaggcc aagtccaagc cggtcgaaga gctgtcgctg tcggacctgg gcctgtcgcc
     2701 cgacgacgcg aagccgacgc agacggttgc ggcggtcaat cccgcgccgg agcgcggcca
     2761 gggcgagatc gtcgaggacg acggaagcgg cgccgcgaag atcgcggact tcctcaaaga
     2821 agcgaaggtg atctgacgtg gcgaagatct gggtgtacgc agagctcgac tcgcacggca
     2881 acctcgagaa gacctcgctc gagatcctga cgaaggcgcg cacgctcggc ggcgaggtcg
     2941 aggcggtcgc gctcggcccc ggcgcgaatg ctgcggccgg cccgctcggt gagcacggcg
     3001 cgacgaccgt ctacgcgtgc gaggacgccg tcttcaccga cttcgtcggc cagccggccg
     3061 cccacgtcct ctacaacctg gtgcaggagc acaagccgaa cctgctgctg ttccccttca
     3121 cctacgacag ccgtgacgtg gcgggccgtc ttgccgccaa gaccggctcc acggtgatgg
     3181 gaagcgtcac cgacgtcctg tccgacacct cggcccagac ccagatcttc ggcggctcca
     3241 agctggtgga tgtcacgctg gagggcccgg acccgaaggt cgccttgatc cggccgaagg
     3301 cgttcgaggc cgagccgtcg ggcggcagcg caaccaccgt cgccgtcacc gccgacgttc
     3361 ccgacaccgc gaagaaggcg aagcgcgtcg agcgcatcga agaggagcag tcgggtccga
     3421 agctcgaaga ggcgcccgtg atcgtgtcgg ggggtcgcgg ccttcaggat ccgtcgaact
     3481 tctcgttgct cgaggagctc gcaggcgcga tcggcaacgc cgcggtcggc gcgacgcgcg
     3541 cggtggtcga cgccggatgg gtcccctacg cgatgcagat cggccagacc ggcaagacgg
     3601 tcaagccgaa ggtgtatctg gcgatcggca tctccggcgc cacgcagcac atcgtgggaa
     3661 tgaagtcctc ggaccgcatc gtcgcgatca acaaggatgc cgaggcgccg atcttccagc
     3721 tcgccgacct cggc
//
LOCUS       DATLFO010000008         5452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590 k141_189,
            whole genome shotgun sequence.
ACCESSION   DATLFO010000008 DATLFO010000000
VERSION     DATLFO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VM841_00225"
     CDS             complement(<1..870)
                     /locus_tag="VM841_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88635.1"
                     /translation="MNDAISKRLRATRQAVMRFRRLRAGRRTKRTRTIVLLLVLASAA
                     GGMAAALDALEPAPHGERLRVDELDALAAAKALTHATFRDEDAQIVGRYTCPPGAAAG
                     VKACLAAAVEARAAAEAAAAGTTAPAEAAAAPQAAAPPAPFVTRRFWMPYPKSDLATG
                     ALIKTAVEAGARVAVDPQVPKAQLRMLTTFVLPLLILAALFALLFTAGRSSGSGIGDV
                     IVFGSLRKGRTRRGRARRVTFDDVAGADHAVAELREIVDYLQNPQRYRDIGALAPKGV
                     LMIGPPGCGKTLLA"
     gene            complement(867..2477)
                     /locus_tag="VM841_00230"
     CDS             complement(867..2477)
                     /locus_tag="VM841_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HVL88636.1"
                     /translation="MRTIRSITQPLFGAIVVAATLALPAVPAYAAHRGCDDPPSPPRT
                     RTARAEPARLEPAPAPVTLANGAAHRIEMRLVRPADPTPVDVFFLIDTTGSMAGAICG
                     VQRDLSSVAVELAESGVDVRFGLGIYRDYPFSPWGSPGDFAYRRVRDLAPLDRELLEA
                     LHNLEAAGGGDGLQSGLAALYQVATGEGQDVNPAGRSAGDIEPGIAPRFRKDSLRLVI
                     NATDVEFREPREEEKSNDYGDYPGPAFDRAVSVLRTRAIMQAGIAISSNAAGSLGRMA
                     SKTGAVAPPGGADCDGDGRADLRAGAPLVCEGQEDSLAGPLVGGQRPVKMANAIVAIV
                     RALRDEATIGFTVEGGDGLLQAVTPRTSPAVDLKRDATLPVSVDVRCDRVHGRKTLRI
                     AATLRGEVIASVPYEVTCLPPPSVVRGVQRAAAHAPSAQQPAQQPASQPQTNPNPNPN
                     TNVQAQADPSAAVGVAVAPQPQGALARAFEQTDGLAGMTARRRSTRPYAAGVLLVTIT
                     AAGCAAFARRPAPATAASRAARPARRRP"
     gene            complement(2574..3221)
                     /locus_tag="VM841_00235"
     CDS             complement(2574..3221)
                     /locus_tag="VM841_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HVL88637.1"
                     /translation="MKRKRSEIIDCLAAEWRSLEALIRGLTPDDLDHPTRCEAFSVRD
                     VAAHSAAIVSDIPAGTTGKADPQQEAAVRRGRTAEQLADEIAEAAPAAEALLRRLPDE
                     IWDGPSPIAGLSVGRAVEAGILEAVVHADDIRAALGRLSERSFGLTVSIEHVAARLEE
                     RGWGPRTIAPEGMQPIDVDGGGDPIGGDPWAFLLAATGRATPAGLGLDDAVNIFA"
     gene            3332..3949
                     /locus_tag="VM841_00240"
     CDS             3332..3949
                     /locus_tag="VM841_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL88638.1"
                     /translation="MVEAAAHLLWAQGFHGTGLQEILARSRAQKGSMYFHFPGGKEEL
                     AGEAMRASGRFVLTGVRGALEHYPDAASGIRAVVDAFATFLRDSSFDLGCPVATVTLE
                     AASKSDVIRTAAQEVYDDWMRVLADRLAADGYPPERCEPLAMLVLSSIQGALILSRAR
                     MDVSPLEMLSREIEGMLGRRPRRRRQPKPGGSSKSSKVKRTGSPS"
     gene            complement(3886..4368)
                     /locus_tag="VM841_00245"
     CDS             complement(3886..4368)
                     /locus_tag="VM841_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88639.1"
                     /translation="MELLWNPRRPTDVNRLAIAALLLAAGCNTSEPVVIPPGTGPVAA
                     TPSSSATVPAATADFTMWISNQGSRHPTIDIEVRIDDSVVVDGSFPAGQHTFFPYALR
                     LGPGTHRLVARTPGGTRIEQTFEVSAEKPRYATLFFWSYEGDPVRFTFELFDEPPGFG
                     "
     gene            4367..>5452
                     /locus_tag="VM841_00250"
     CDS             4367..>5452
                     /locus_tag="VM841_00250"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HVL88640.1"
                     /translation="MRRYHDAVLVRWGKARSARALALCLLVGMLPATAHAGWSDAEYG
                     RQVGRLERQVSSMDPARGSTPGYAVLRNGRFVYIRNDNRAMLPASLQKLLVTTAAIVK
                     LGPQHRFATRVVSASPITDGTIAGSMTLVGGGDPTLSTQSFTGYYRRSAPGGKVSAIS
                     FVPTIELLADGLVKNLGLRRIDGNLVADESVFDSHRWQRGWQSSYQRGEIDVGNLSGL
                     TVNLGLGDPQGKTVAASPALLAATYLRDALKARGVEITGSVAMGKAAGTDNEITRISS
                     APLREIVFWTNRWSANYPAEMLLKGLGAAFGGGGSTVNGAAVLKQALAASRIDVRDLV
                     VEDGSGLSTLNRASPRVLATVLALMLSM"
BASE COUNT          798 a         1913 c         1942 g          799 t
ORIGIN      
        1 tgcgaggagc gttttgccgc aaccgggcgg cccgatcatc agcacgccct tgggggcgag
       61 cgcgccgatg tcgcggtagc gctgcgggtt ctgaagatag tccacgatct cgcgaagctc
      121 ggcgacggca tggtccgcgc cggcgacgtc gtcgaaggtg acccgcctcg cgcggccgcg
      181 tcgcgtgcgt cccttgcgca gcgagccgaa cacgatcacg tcgccgatgc ccgagcccga
      241 cgagcggccc gcggtaaaca gcagcgcgaa cagcgccgcg aggatcagca gcggcagcac
      301 gaacgtggtg agcatgcgca gctgcgcttt ggggacctgc ggatccaccg caacgcgcgc
      361 gcccgcctcg acggcggtct tgatcagcgc gccggtcgcg agatccgact tgggatacgg
      421 catccaaaac cggcgcgtca cgaacggcgc cggcggtgcg gcggcctgcg gcgcggccgc
      481 cgcttcggcc ggcgcggtgg tcccggcagc cgcggcctcc gcggccgcgc gggcctcgac
      541 ggccgcggcc agacacgcct ttacgccggc cgcggcaccg ggcggacagg tgtagcggcc
      601 tacgatctgc gcatcctcgt cgcggaacgt cgcgtgcgtg agcgccttcg cggccgcgag
      661 cgcgtcgagc tcgtccactc gcagccgctc gccgtgcggc gccggctcga gcgcgtcgag
      721 cgccgcggcc atgccgccgg ccgcggatgc cagcaccaga agcagcacga tcgtgcgcgt
      781 gcgcttcgtg cggcggcctg cgcgtagccg gcggaaccgc atcacggcct gacgcgtggc
      841 acgcagccgc tttgagatcg cgtcgttcat ggtcgtctcc ttgcgggtcg agccgcgcgg
      901 gaagcggcgg tggcgggtgc gggacgccgt gcgaacgccg cgcagccggc ggcggtgatc
      961 gtcacgagca gcacgccggc ggcgtacggc cgggtgcttc tgcgtcgtgc cgtcatcccg
     1021 gcgagcccgt cggtttgctc gaacgcgcgg gcgagcgcgc cctgcggctg gggtgcgacc
     1081 gcgacgccga cggccgcgct cgggtccgcc tgcgcttgca cgttcgtgtt cgggttggga
     1141 ttcgggttgg tctgaggctg cgaggccggc tgctgcgcgg gctgctgagc gctcggcgcg
     1201 tgcgcggcgg cgcgctgcac gccccgcacc accgacggcg ggggcaggca ggtcacctcg
     1261 tagggcacgg aggcgatgac ctcgccacgc agggtcgcgg cgatgcgaag cgtcttgcgt
     1321 ccgtgaacac gatcgcagcg aacgtcgacc gagaccggaa gcgtcgcgtc gcgcttgaga
     1381 tcgaccgcgg gagacgttcg cggcgtaacg gcctgcagca gcccgtcgcc accttcgacc
     1441 gtgaagccga tggtggcctc gtcgcgtaac gcgcgcacga tggccacgat cgcgttcgcc
     1501 atcttgaccg gacgctggcc gccgaccaac ggccctgcca gcgaatcctc ctgtccttcg
     1561 cacaccagcg gcgccccggc gcgaaggtcg gcgcggccgt cgccgtcgca gtcggcgccg
     1621 cccggcgggg cgaccgcgcc ggtcttcgac gccatccggc cgagcgagcc ggcggcgttc
     1681 gacgagatcg cgatgccggc ctgcatgatc gcgcgagtcc gcaggacgct caccgcccgg
     1741 tcgaatgccg ggccgggata gtcgccgtag tcgttgctct tctcctcttc tctcggctcg
     1801 cggaactcga cgtcggttgc gttgatcacc agacgcagcg agtccttgcg gaaccgcggc
     1861 gcgatgcccg gctcgatgtc gcccgccgac ctgcccgcgg ggttgacgtc ctgtccttcg
     1921 ccggtggcga cctgatacag cgcagcgagc cccgactgca gaccgtcgcc acccccggct
     1981 gcctcaaggt tgtgcagcgc ctccagcaat tcgcggtcca gcggtgcgag gtcacgcacg
     2041 cgccggtagg cgaaatcgcc gggggatccc cacggcgaga acgggtagtc gcggtagatg
     2101 ccgaggccga atcgcacgtc gacgcccgac tcggcaagct cgaccgcgac cgaagacagg
     2161 tcgcgctgca cgccgcagat cgcgccggcc atgctgccgg tcgtgtcgat caggaagaag
     2221 acgtccaccg gcgtcggatc ggccggacga acgagcctca tctcgatgcg atgcgccgcg
     2281 ccgttggcga gcgtcaccgg cgcgggcgcc ggctcgagcc gcgcaggctc ggcgcgagcc
     2341 gttcgggttc gcggcggcga cggcgggtcg tcgcagcccc ggtgcgcggc gtaggcgggg
     2401 acggcaggca gagccaacgt cgcggcaacg acgattgcgc cgaaaagcgg ctgcgtgatg
     2461 gatcgaatcg ttctcatggt ctccctcacc gaggaagacc ccaccgtcgc cgtttcgtaa
     2521 caggcgacgg tgggatgccc gagggagacc ggccctgcga aacccgtgag acgctaggcg
     2581 aagatgttga cggcgtcgtc cagaccgaga cccgccggcg tcgcccgacc ggttgccgcg
     2641 agcaggaagg cccacgggtc gccgccgatc ggatcgccgc cgccgtccac gtcgatcggc
     2701 tgcatgccct cgggcgcgat cgtgcgcggt ccccagcctc gctcctcgag gcgggccgcc
     2761 acgtgctcga tcgagacggt caggccgaag ctgcgttcgg agagtctgcc gagcgccgcg
     2821 cggatgtcgt cggcgtggac caccgcctcc aggatccccg cctcgacggc ccggccgacg
     2881 ctcagcccgg cgatcgggct gggaccgtcc cagatctcgt cgggcaggcg ccggagcagc
     2941 gcctcggccg cgggcgccgc ctcggcgatc tcgtcggcga gctgttcggc ggttcgtccg
     3001 cgccggaccg ccgcttcctg ctgcggatcg gcctttccgg tggttcccgc cgggatgtcg
     3061 gaaacgatcg cggccgagtg cgcggcgacg tcgcgaaccg agaaggcctc gcatcgcgtc
     3121 ggatgatcca ggtcgtccgg cgtcaaaccc cggatgagcg cttcgagcga gcgccattcg
     3181 gcggcgaggc aatcgatgat ctcggagcgt ttccgcttca cgacgaatcc cccctctggg
     3241 tagaccgatc ggtcaagcgg tatactagac cggtcggtca acatccgtca aggagcctgc
     3301 cctgtccgag cacgccgata ccaagactcg catggtcgag gctgccgcgc acctgctgtg
     3361 ggcgcaaggc tttcacggca ccggcctgca ggagatcctc gcccgcagcc gcgcccaaaa
     3421 gggttcgatg tactttcact tccccggcgg caaggaagag ctcgccggag aagcgatgcg
     3481 cgcctcaggc cgcttcgtgc tcaccggcgt gcgaggcgcg ctcgagcact atccggacgc
     3541 ggcgtcgggg atccgcgccg tcgtcgacgc cttcgccacg ttcctgcgcg actccagctt
     3601 cgatctgggc tgcccggtcg cgaccgtgac gctggaggcg gcaagcaagt ccgacgtcat
     3661 tcgcacggcc gcgcaagagg tctacgacga ctggatgcgc gtgctcgctg accggctcgc
     3721 cgccgacggg tatccgccgg agcggtgcga gccgctcgcg atgctggtgc tgtcgtcgat
     3781 ccagggggcg ctgatcctga gccgagcgcg catggacgtc tcgccgctgg agatgctgag
     3841 ccgcgagatc gaaggcatgc tgggccggcg gccgcgacgg cggcgtcagc cgaagccggg
     3901 cggctcgtcg aagagctcga aggtgaagcg gaccggatcg ccctcgtagc tccagaagaa
     3961 cagcgtggcg tagcgcggct tctcggcgga cacctcgaag gtctgctcga tcctggttcc
     4021 cccgggcgtt cgtgcaacca gccggtgtgt gcccggaccg agccgcagcg cgtacgggaa
     4081 gaaggtgtgc tggccggcgg gaaacgagcc gtcgacgacg accgaatcgt cgattcgaac
     4141 ctcgatgtcg atcgtcgggt ggcgagagcc ctggttgctg atccacatgg tgaagtcggc
     4201 cgtggcggcc ggcacggtcg cggacgaaga gggcgtcgcg gcgaccgggc ccgtcccggg
     4261 cggaatcacc acgggctcgc tcgtgttgca gcccgcggca agaagcaacg cggcaatcgc
     4321 caaccggttc acatcagttg gacgacgggg gttccacagg agttccatgc gccgctatca
     4381 tgacgcagtc ttggtcaggt ggggcaaagc tcgctcggca cgtgcgctcg cgctgtgttt
     4441 gctcgtcggc atgctcccgg ccaccgctca cgccggctgg tccgacgccg aatacggccg
     4501 ccaggtcggc cgcctcgagc ggcaggtctc gtcgatggac cccgcgcgcg gatcgactcc
     4561 cggctatgcg gtcttgcgca acggccggtt cgtctacatt cgcaacgaca accgcgcgat
     4621 gctgcccgcg tcgctgcaga agctgctcgt caccacggcc gcgatcgtca agctcggtcc
     4681 ccagcatcgc ttcgcaaccc gggtggtctc ggcgtcgccg atcacggacg gaacgatcgc
     4741 cggctcgatg acgctcgtcg gaggcggcga tccgacgctt tccacccagt cgttcaccgg
     4801 ctactaccgc cgcagcgcac ccggcggaaa ggtatcggcg atctcgttcg tgccgaccat
     4861 cgagctgctc gccgacgggc tcgtcaagaa cctcggcctt cgccgcatcg acggaaactt
     4921 ggtcgccgac gagtcggtct tcgactcgca tcggtggcaa cgcggatggc aaagctcgta
     4981 tcagcgcggc gagatcgacg tgggcaacct gagcggcttg acggtcaacc tcggcctcgg
     5041 cgacccgcaa ggaaaaaccg tcgcggcatc gccggcgctc cttgcggcga cctatcttcg
     5101 tgacgcgttg aaagcccgcg gcgtcgagat caccggctcg gtcgccatgg gcaaggccgc
     5161 cggcaccgac aacgagatca cgcgcatcag ctcagcgccc ctgcgcgaga tcgtgttctg
     5221 gacgaaccga tggagtgcga actatcccgc ggagatgctg ctcaagggcc tcggtgcggc
     5281 gttcggcggc ggcggctcga ccgtcaacgg cgcggccgtg ctcaagcagg cgctcgccgc
     5341 gagcaggatc gacgtccgcg acctcgtcgt cgaggacggc agcggcctgt cgacgctcaa
     5401 tcgcgcctcg cctcgcgtgc tcgcgaccgt gctcgcgctg atgctgtcga tg
//
LOCUS       DATLFO010000009        10485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_79437, whole genome shotgun sequence.
ACCESSION   DATLFO010000009 DATLFO010000000
VERSION     DATLFO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79437"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VM841_00255"
     CDS             complement(<1..257)
                     /locus_tag="VM841_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVL88641.1"
                     /translation="MTSLIYSPEATAEVRAIHDWYERESAGLGRALLEDLRIAEEAIA
                     RSPFAFRVIRRETRRYLLARFPYQLLFTVRKDTVVIVACLHA"
     gene            complement(254..541)
                     /locus_tag="VM841_00260"
     CDS             complement(254..541)
                     /locus_tag="VM841_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HVL88642.1"
                     /translation="MATIEPFAGLSSRFGSCHTELMAKRAVDISQLNAEERLALIEEL
                     WKSLDQEERDAIPLTPEQEAELDRRLDALDRDGVQGLSPQELREFVKRRSP"
     gene            complement(702..1862)
                     /locus_tag="VM841_00265"
     CDS             complement(702..1862)
                     /locus_tag="VM841_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HVL88643.1"
                     /translation="MFDAVSAPARATLVAEALRRADAAIAEAIRLIGSGNGIAAATGL
                     PVERILEIDGRLTGADARMLSAASDALRSMPSLAAAFERGTVSWSQVRSIVCAVRGVP
                     AAVRDQIDALIAERAVTLAEAEPYRLIELVDDAVMRLRAGRLQSRQDRQVAAEFLAVQ
                     GRFDGGASIYGEFGADAAATVLEALDAHADAPRRSDAHPDAQPDAGHAPSRARQRAEA
                     FVRLCEAGLSGRGGTRPRPRVLATVDVCQFQDGRDGAMRVLWALTGRAPRLSRVAAEE
                     TMCDATIVPVVFDRGRVVDVGDETTRFSPKVRRAIIARDQGCRAPGCRMPAKWSDVHH
                     IVPGVGSTADDGILLCRADHRRAHRQRWKITLHTDGSIAYRIRGRTYLSLPP"
     gene            complement(1948..2514)
                     /locus_tag="VM841_00270"
     CDS             complement(1948..2514)
                     /locus_tag="VM841_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88644.1"
                     /translation="MRKILVTAVLAGAFWLGGSLPVWACSCAGSGPPVHFDHADAVFV
                     ASLENRRDPNGDQTIISSADPIDYEMYVESVQKGDVAEQVVVRSARNGASCGFTFVRG
                     IRYQIFARQDGDRFLTSLCEGTAVLGAQVYEPNGSDGPARTGLSGEVGPPPGEAPGRA
                     KPLAGAVAGLLIAGLGVALARAARRSVS"
     gene            complement(2571..3263)
                     /locus_tag="VM841_00275"
     CDS             complement(2571..3263)
                     /locus_tag="VM841_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88645.1"
                     /translation="MSHFTCKDCTALAAELALGVAGGEERAHALSHIGSCPSCRAEVE
                     EMSMAADAMLMLAPQVEPPIGFEARVMARVSKPRRRVSIKTMLAAAVIAAVASAATGM
                     VVYAGGSHERHLATQYEKLVQALGGKTLRAAELRALDGTVVGKVYAYDGKPSWLFLVL
                     QDREGSGQYEVQLEPVSGDAITLAGLTIKSGRASWATTASVQIDDLESVSILDSTGVP
                     IYGADFKKQSGK"
     gene            complement(3260..3910)
                     /locus_tag="VM841_00280"
     CDS             complement(3260..3910)
                     /locus_tag="VM841_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL88646.1"
                     /translation="MGGVPWDGARAINPFPAALRSQSMWAVSDESLLAGLGTGDPEAV
                     AAFIRRFQRRVYGLAVSIVGDAVLAEDIAQETFARAWRHAQAYDPRRGPVAAWLLSIC
                     RNLSIDTLRPQRAEPTDPEAILAMQLPAAGIEPSDAAVRADDAARLREAIGRLPQDQR
                     RSIVLAAFYGRSAREISETEGIPLGTAKTRIRTAMIKLRAAMTKEREEALKDHEIE"
     gene            complement(3953..4678)
                     /locus_tag="VM841_00285"
     CDS             complement(3953..4678)
                     /locus_tag="VM841_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88647.1"
                     /translation="MSQQDFVPAFQVSGGQDPDLPPPPPEPAPEPFAPPEPPPDPKTE
                     GRAIASLTAKVKLLGERIAAQETLTARMSASMERVESSVAEHTKRTQPMLETLSAELA
                     RQGAMIESIKTDTESSLDLHAQRVSEFEARLATVLREMRELVASKIESYDIAIGDRLE
                     EARHAVEDQLATFEERVEDLVRRFAAAAIPDEMRDKVEDLSGRFIHAETALAATRSSV
                     EFLRAEVGSLTMLLRKLAPDENV"
     gene            4835..5857
                     /locus_tag="VM841_00290"
     CDS             4835..5857
                     /locus_tag="VM841_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL88648.1"
                     /translation="MRPTKFMLAAAIMAALVVPLPGRASATVQRTAVTIPVYNPTTYE
                     FLTVHGGLFQRSDRTACGNSVLVLVHGLSYGEFAWDFPVQRDTYSVARSLAAAGYPTL
                     AIDLPGYDSAQPDTRDKPNGYTLTVQAYAHMVGQVETALRYGNYGGPAFSKVGLMGHS
                     AGTEITELAAGLGFADPDVYIPTGYHHVPSQRIVTDFFTGDFIRAAQSDYEYFGGDEQ
                     GRTEYMYAPLPSDNVDETIVAADNALANLTPSGEVYSIGPQPSKAVMGLIDAPVLFVI
                     GDTDLLFPVEFVDAELNLFASAPSVTKLIVEDAGHSFMLHKNAPQTNAAIAAWLATQS
                     SVFPAC"
     gene            5998..6177
                     /locus_tag="VM841_00295"
     CDS             5998..6177
                     /locus_tag="VM841_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88649.1"
                     /translation="MDRTFPEIDPRTRQLLERDFLYEHPESFRAGVLAAVQILSERAD
                     APDQDAPLSVRRAGA"
     gene            6468..7580
                     /locus_tag="VM841_00300"
     CDS             6468..7580
                     /locus_tag="VM841_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL88650.1"
                     /translation="MAQDLATEFVADLYRRERSGIARSVARIAGESHAEDLVHDAFVA
                     YLTKAPWAEKPGAWVSTVARNRALNTLRRPKLVPLSGEEPAADLEPIDSEREAVRHVI
                     ATALSAIHPRSLTAIRMKFFEGAGYAEIAEALGVRVSQAHVIVHRSLRRLGRELIRQM
                     ADAHGAGQCAVALTRLAGLATAAEDRHASGHDEGPCVLCAPAWDEIQALRIGGLIAPL
                     IAFKDQIRSLTEKLAGRITVRGSLASDAVSRATTAFVAMGVAAASMTPVAATLPSESQ
                     RPASRIEAPSAGTNLTAEAPAAPAADQAPAAPPGAPPQRAADRSAPTTTVGEAGRVTP
                     PTDREPGHVGNDDAGVLVCLDPTKPCEPPPPGGREG"
     gene            7582..9354
                     /locus_tag="VM841_00305"
     CDS             7582..9354
                     /locus_tag="VM841_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88651.1"
                     /translation="MKRFVAAFTVAALAFAIPAASPASVAECRVLTDATGDVAGPLGT
                     ARPSRTAGASVDLTGVTMHGNAPGVLVTISVTDLKAPPTVGTGGAVYTTFWNVGADRF
                     FAQALRVGREWSFSAGRAAGIDDAIPQQGTPVDGRITGADIRLRVPALLAGSPRVGGV
                     LQQVRAKTQETLWQTPEALAGQTSIQPVAAMADAAGGPEGGRFLIGGACLPGISADGE
                     RCLVTTDLPGDATTGALPAAVRGTGADRELRDAPASAADPATDILALQMGSNVSTLII
                     EIALARLDAAMPTGADAQRWEVSWNDGRRVTTAFAERRTTGATFGYLADGAEYATTGS
                     LDRVGAVVRILVPRHEIGAAEATRMNSIVARGMLVTGAVRDVRDIAPDIQAWSVQYVA
                     GVSCVDQARAACPVALDVNGDAGPVVDHEGRTVPAYQPASDLLAVGASAPDGTLVLSV
                     RVADAGAPPPDGFTMQGWTVSWTWRGIRYMAQAERTRDRVVFRTRALGPADKYGKPTG
                     PAFIGTDEASGSIDAQAGVIRIVLRREYAGSPYDGAALTDMGALSWVMNDGKVAEGAY
                     GPARHVTVDETPVAPYRMGLGCSA"
     gene            complement(9379..10167)
                     /locus_tag="VM841_00310"
     CDS             complement(9379..10167)
                     /locus_tag="VM841_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVL88652.1"
                     /translation="MRTIWKGAISFGLVTIPIRVFTAVEEKSLKFNQLHEPDQGRIKY
                     KRVCSIDGEEVPSDQIVKGYEYEKDHYVVLTEDELDRGLSGSRAIEVLKFVRQEEIDP
                     ILFKSSYYLSPDGATGTKAYALLRTALGEEGLVALVKVQWRDKEHLATLRVKDDVFVL
                     ETMYWPDEIREPDFEELKDDQQVRDQEVQMARMLIESMTDVFKPDEFRDTYREKLEAL
                     VSQKIEGKEIAVVADEGEAAPVVDLMEALRASVEAVNKDKSATG"
     gene            10266..10445
                     /locus_tag="VM841_00315"
     CDS             10266..10445
                     /locus_tag="VM841_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88653.1"
                     /translation="MTGIRRFAFATGLVAVWVTEPLDWSVIAPTREASLTPTTAHPPR
                     TARDRLIARFVLRRR"
BASE COUNT         1640 a         3700 c         3521 g         1624 t
ORIGIN      
        1 gcgtggaggc atgcgacgat cacgaccgtg tccttacgaa cggtgaacag caattggtag
       61 ggaaaacgcg cgagcaaata gcgcctggtt tcgcgtcgaa tgacccggaa ggcgaatggc
      121 gaccgggcga tcgcttcttc agcaattcta aggtcttcga ggagagcgcg acctagtccg
      181 gcgctttcgc gttcgtacca atcgtgaatg gcccgaacct cagcagtcgc ttccgggctg
      241 tagattaaag aggtcacggg ctgcggcgtt tgacgaactc gcgaagctcc tgtggggaga
      301 gaccttgcac gccgtcccga tctaacgcat caagccgacg atcgagctcg gcttcctgct
      361 caggcgtgag cgggatcgca tcccgctcct cttgatcgag gctcttccaa agctcttcga
      421 tcagagcaag gcgttcttcg gcgttcaact ggctgatatc aaccgctcgc ttcgccatga
      481 gttcagtatg gcacgagccg aaacgcgagg acagtccggc gaacggttcg atcgtcgcca
      541 ctctctcgat cgcctcttac ctgatcaaga gcccggtggc cctaaacgga ttcgaaccgc
      601 gcgcgggcat ggcgatggac ggcccacacg tttgggccgc tggggaccga attggagatg
      661 ctgcgagaag ctagccggga aaggcggaac gggaccttcg gctatggggg aaggctgagg
      721 taggtgcggc cgcgaatcct gtatgcgatg gagccgtcgg tgtgaagggt gattttccag
      781 cgttgtcggt gggctcggcg gtggtcggcg cggcacagaa gaattccgtc atcggcggtg
      841 ctgcccacac ccggcacgat gtgatggaca tcgctccact tcgcgggcat tcggcagccg
      901 ggcgcgcggc atccttgatc gcgggcgatg atggcgcgac gcaccttcgg tgagaagcgc
      961 gtggtctcgt cccccacatc gacgacgcgg ccacggtcga acacgacggg aacgatggtg
     1021 gcgtcgcaca tggtctcttc ggcggcaacg cggctcagac gcggcgcgcg cccggtcagc
     1081 gcccacagca cgcgcatcgc tccatcgcga ccgtcctgga actggcacac atcgacggtt
     1141 gcgagaaccc gcggtcgtgg gcgcgtgcca ccccttccgg acaggccggc ctcgcacaga
     1201 cgcacgaacg cttcggcgcg ctgacgcgcg cggctgggtg cgtggcccgc gtcaggctgg
     1261 gcatcgggat gggcatcgga acgacggggg gcgtctgcat gcgcatccaa cgcttcgagg
     1321 acggtcgcgg ccgcgtcggc gccgaactct ccgtagatgc tggccccgcc gtcgaatcgg
     1381 ccttggacgg cgaggaactc tgcggcgacc tggcgatcct gccgggattg cagccgtccc
     1441 gcccgcaggc gcatgacggc gtcgtccacg agctcgatca gccggtacgg ttcggcctcg
     1501 gcaagcgtga cggcgcgttc tgcgatcagc gcgtcgatct gatcgcgcac ggctgcggga
     1561 acgcctcgga ccgcgcagac gattgaccgc acctgcgacc acgacaccgt gccgcgttcg
     1621 aacgcggcgg cgagcgacgg catggaccgc agcgcatccg acgccgccga aagcattcgc
     1681 gcgtccgcgc cggtcagccg gccgtcgatc tcaaggatcc gttcgaccgg caagccggtt
     1741 gcggccgcaa tgccgttgcc gctgccgatc agccgaatcg cctcggcgat cgcggcatcg
     1801 gcgcgacgca gcgcctcggc gacgagcgtc gcgcgagcgg gggcactcac tgcatcgaac
     1861 atgtccgcga tactacaatg ggcctatgac aggaacgatg caatcgcagc gtccgagaaa
     1921 gcgactgcga agatcacacc gggcacatca cgaaaccgac cgtcgcgcgg cacgggcaag
     1981 cgccacccca agcccggcga tcagcagccc ggcgacggcg cccgcgagcg gcttggcgcg
     2041 gccgggcgcc tcgcccggcg gcggccccac ctctccggac agtcctgtcc gggccggtcc
     2101 gtcggaaccg ttcggttcgt agacctgcgc gcccagcacc gcggtccctt cgcagagcga
     2161 ggtcaggaac cgatcgccgt cttgccgcgc aaagatttga taccggatgc ctcgcacgaa
     2221 cgtaaagccg caggatgcgc cgttccgtgc ggagcgaacc acgacctgct cggccacatc
     2281 gcccttctga accgactcga cgtacatctc gtagtcgatc gggtccgccg aggagatgat
     2341 cgtctggtcg ccgttcggat cccgccggtt ctcgagcgag gcgacgaaaa ccgcgtcggc
     2401 atggtcgaag tggacgggcg gcccgctgcc cgcgcacgag catgcccaca ccggcagcga
     2461 gccgcccagc cagaacgcac cggccaacac ggcggtcacg aggatcttgc gcatgcggaa
     2521 acccccttta cggatcagac gatacgccgc gtgcggaggt tccgcgaggg ctacttgccg
     2581 gattgcttct tgaaatcggc gccgtagatc ggcacgccgg tcgagtccag gatcgacacg
     2641 ctctccagat cgtcgatctg gaccgaggcg gtcgtggccc aagacgcgcg gcccgacttg
     2701 atggtcaggc ccgcaagcgt gatcgcgtca cccgacaccg gctcgagctg aacctcgtac
     2761 tggccgctgc cttcgcggtc ctgaaggacc aggaacagcc acgacggctt gccgtcgtag
     2821 gcgtagacct tgccgaccac ggtgccgtcg agcgcgcgaa gctcggccgc gcgcagcgtc
     2881 ttgccgccga gcgcctggac cagcttctcg tactgggtgg ccagatgacg ttcgtggctg
     2941 ccgccggcgt agacgaccat gccggtcgcg gccgaggcga ccgccgcgat gaccgcggcc
     3001 gccagcatcg tcttgatcga cacgcggcgg cgcggcttgg acacgcgcgc catcacgcgc
     3061 gcctcgaagc cgatcggcgg ctcgacctgg ggcgcgagca tcagcatcgc gtccgctgcc
     3121 atcgacatct cctccacctc ggcccggcac gacgggcacg acccgatgtg ggacagggca
     3181 tgcgcgcgct cttcgccgcc ggccacgccg agcgccagct ccgcggccag cgcggtgcag
     3241 tccttgcagg tgaagtggct cattcgatct cgtgatcctt caacgcttct tctcgctctt
     3301 tcgtcatggc cgcgcgcaac ttgatcatcg cggtccgaat cctggtcttg gccgtcccca
     3361 gcgggatgcc ctcggtctcg ctgatctcac gagccgagcg gccgtagaag gcggcgagca
     3421 cgatcgagcg ccgctggtcc tggggaagcc ggccgatcgc ctcgcgcagc cgcgccgcgt
     3481 cgtcggcgcg caccgccgcg tccgacggtt cgatgccggc cgcgggaagc tgcatcgcca
     3541 ggatcgcctc cgggtcggtc ggctcggccc gctgcggccg gagcgtgtcg atcgacaggt
     3601 tgcggcagat cgacagcagc caggccgcaa ccggaccgcg gcgcggatcg taggcctgcg
     3661 cgtgacgcca ggcccgcgcg aaggtctcct gggcgatgtc ctcggcaagg accgcgtcgc
     3721 cgacgatgga cacggcaagg ccgtagaccc gccgctggaa gcggcggatg aaggcggcga
     3781 cggcctcggg atcccccgtg ccgagccccg ccaggaggct ttcgtccgat acggcccaca
     3841 tactctgact acgcagcgcg gccgggaaag gattgatcgc gcgcgcgccg tcccatggta
     3901 cgccgcccat caaggcgaga tcgagcggcg tgacccccga ttccgggcag aatcagacgt
     3961 tttcgtccgg ggcgagcttg cgaagcagca tcgtgagcga gccgacctcg gcgcgcagga
     4021 actccaccga ggagcgtgtc gccgccagcg ccgtctcggc gtggatgaac cggccggaca
     4081 ggtcctcgac cttatcgcgc atctcatcgg ggatggcggc ggccgcgaag cggcgcacga
     4141 gatcctcgac gcgctcctcg aaggtggcga gctggtcttc gaccgcgtgc cgcgcctcct
     4201 cgagccggtc gccgatcgcg atgtcgtagg actcgatctt gctcgccacc agctcgcgca
     4261 tctcgcgcag gaccgtcgcg agccgcgcct cgaactcact gacgcgctgg gcgtgaagat
     4321 cgagcgagga ctcggtgtcg gtcttgatcg actcgatcat cgcgccctga cgcgcgagct
     4381 cggccgacag cgtctcgagc atcggctggg ttcgcttcgt atgctcggcc acggaggatt
     4441 cgacgcgctc catcgaggcc gacatgcgag cggtgagcgt ctcctgcgcg gcgatccgct
     4501 cgccgagcag cttgaccttg gcggtcagcg acgcgatcgc gcggccctcg gtcttcggat
     4561 cgggcggcgg ctcgggcggc gcgaacggtt ccggagcggg ctccgggggc ggcggcggga
     4621 ggtcgggatc ctgaccgccc gagacctgaa atgcgggaac gaaatcctgc tggctcacgc
     4681 cgcggagcct accgcagcgt gagcggaaat cccgcgaaga acgaggcgag cccggcggcg
     4741 aatcgggcat cacggcagga tcgggccgcc cggcgtcgaa gggagcccca acgatccgca
     4801 tcgacccacg gacgccgaca cggaaggatc cgaagtgcgc cccaccaagt tcatgctcgc
     4861 ggccgcgatc atggccgcgc tcgtcgtgcc cctgccgggc cgggcgagcg ccaccgtcca
     4921 gcggacggcg gtcacgatcc cggtctacaa cccgacgacc tacgaattcc tgaccgttca
     4981 cggtggcctg ttccaacgct ccgaccggac cgcgtgcggc aacagcgtgc tggtgctcgt
     5041 ccacggcctg tcctacggag agttcgcctg ggatttcccg gtgcagcgcg acacctactc
     5101 ggtcgcccgc tcgctcgcgg cggccggcta cccgacgctg gcgatcgacc ttccgggcta
     5161 cgactcggcc cagcccgaca cgcgcgacaa gccaaacggc tacaccctca cggtccaggc
     5221 gtacgcgcac atggtcggcc aggtcgagac cgccctgcgc tacggcaact acggcggccc
     5281 cgcgttctcg aaggtcgggc tgatgggcca ctcggcggga accgagatca ccgagctggc
     5341 cgccgggctc ggattcgccg acccggacgt ctacatcccg accggatacc atcacgtgcc
     5401 ctcgcagcgg atcgtcaccg acttcttcac cggcgacttc atccgcgccg cgcagagcga
     5461 ctacgagtac ttcggcggcg acgagcaagg ccggaccgag tacatgtacg cgccgcttcc
     5521 gagcgacaac gtggacgaaa ccatcgtggc cgccgacaac gcgcttgcca acctcacgcc
     5581 gtcgggcgag gtgtactcga tcggcccgca gccctcgaaa gcggtcatgg gcttgatcga
     5641 cgctccggtg ctgttcgtga tcggcgacac cgacctgctg ttcccggtcg agttcgtcga
     5701 cgccgagctg aacctgttcg cgtccgcgcc gagcgtaacc aagctgatcg tcgaagacgc
     5761 cggccattcg ttcatgctcc ataagaacgc gccgcaaacg aacgccgcca tcgcagcgtg
     5821 gctggcgacg caatcgagcg tatttccggc gtgttgaaga aacccaaagt ccctgcgtgc
     5881 ggcccgggca agggccgcac gcttgatttt ggggcctgtt taccctccgt tcactcatag
     5941 gaaattcatt cgcaatgtgt gtgctgtagc ctgacgttcg actgaccgga ggcccccgtg
     6001 gaccgcacct tccccgagat cgacccgagg acgcgacagc tcctcgaacg tgacttcctc
     6061 tacgagcacc cggagtcgtt ccgggccggc gtgctcgccg ccgtgcagat cctgtccgag
     6121 cgcgccgacg cgcccgatca ggacgccccc ctcagcgttc gccgcgccgg ggcctagccc
     6181 cccgttatcg gccccgggcc ttcccggggc cgtccctttc ggcggagcga tctcccgaaa
     6241 ggtcggtctg acccattttt tccctcctgt ccgttcggct gcgtcttcgc aggtcacggc
     6301 ctatccgcac cctggtcagc gagcgcccga gattcccggt tggaaaattt atctaccgct
     6361 atgtgacaga ttcgaagtag tctcgtttcg actctatgac gggggtttga ggggaccgtc
     6421 gggggagccg ggttgaggga cccggaacgg acgaggggag gtctgtcatg gctcaggacc
     6481 tcgcgacgga gttcgtcgca gatctgtatc gccgtgaacg ctccgggatc gcacggtcgg
     6541 ttgcgcgcat cgccggggaa tcgcacgccg aagacctggt ccacgacgcg ttcgtcgcgt
     6601 atctgacgaa agcgccttgg gccgagaagc cgggcgcatg ggtctcgacg gtcgcccgca
     6661 accgcgcctt gaacacgctg cggcggccca agctcgtgcc gttgtcgggc gaagagcccg
     6721 ccgccgacct cgagccgatc gactcggagc gcgaggccgt tcgccacgtc atcgcgaccg
     6781 cgctgtcggc gatccatccc cgctcgctga ccgcgatccg gatgaagttc ttcgaagggg
     6841 ccggctacgc cgagatcgcc gaggcgctcg gcgtgcgcgt gtcgcaagcg cacgtgatcg
     6901 tgcatcgctc gctccggcgt ctcggacgag agctgatccg ccagatggcc gacgcccacg
     6961 gcgccggaca gtgcgccgtc gccctcacca ggctggccgg gctcgcgacc gccgcggagg
     7021 accggcacgc ctcgggacac gacgaagggc cgtgcgtgct gtgcgccccc gcctgggacg
     7081 agatccaggc gctgcggatc ggcggcctca tcgccccgct catcgcgttc aaggaccaga
     7141 tccgctccct caccgaaaag cttgccggcc gcatcaccgt tcgaggctcc ctcgcctccg
     7201 atgcggtgtc gcgggccacg accgccttcg tcgcgatggg agtcgcggct gcttccatga
     7261 ctcccgtcgc tgcgacgctc ccctcggagt cgcagcgacc ggcgtcgcgg atcgaagccc
     7321 cgtcggccgg cacgaacctc accgccgaag cacccgccgc gcccgccgcg gaccaggctc
     7381 ccgccgcgcc gccgggcgcg ccgccgcaac gcgcggccga ccgttcggca cccacgacca
     7441 cggtcggcga ggccggccgc gtcacgccgc cgacggaccg ggagccgggc cacgtcggca
     7501 acgacgacgc cggcgtgctg gtctgcctgg atccgaccaa gccgtgtgaa ccgccgccgc
     7561 ccggtggcag ggagggatga cgtgaagcgt ttcgtcgcag cattcaccgt cgccgcgctc
     7621 gcgttcgcga tcccggccgc ctcgccggcg agcgtggccg aatgtcgcgt cctgaccgac
     7681 gccaccggcg acgtcgccgg gccgctcggc actgcccgcc cgtcgcgcac cgccggcgcc
     7741 tccgtggacc tgaccggcgt caccatgcac ggcaacgcgc ccggcgttct ggtcacgatc
     7801 tcggtcacag acctcaaggc accgccgacg gtcggaaccg gcggcgcggt ctacaccacc
     7861 ttctggaacg tgggcgcgga ccgattcttc gcccaagcgc tgcgcgtggg ccgcgaatgg
     7921 tcgttctcgg ccggacgggc cgccggcatc gacgatgcca tcccccagca gggcacgccg
     7981 gtcgacggtc gcatcaccgg agccgacatc cggctgcgcg tgccggcgct gctcgccgga
     8041 tcgccgcgcg tcgggggcgt gcttcaacag gtgcgcgcca agacccagga gaccttgtgg
     8101 caaacgccgg aagcgctcgc cggacagacc tcgatccagc cggtcgcggc gatggcggac
     8161 gcggccggcg ggccggaagg gggccgcttc ctgatcggcg gcgcctgcct gccgggcatc
     8221 tctgccgacg gcgagcgctg cctggtcacc accgacttgc cgggcgacgc cacgaccggc
     8281 gcgctgcccg cggccgtgag gggaacgggc gcggatcgcg agctgcgcga cgctcccgcc
     8341 tcggcggccg acccggcgac cgacatcctc gcgctacaga tgggctcgaa cgtctccacc
     8401 ctgatcatcg agatcgccct cgcgcggctc gacgccgcga tgcccaccgg cgccgacgcg
     8461 cagcgctggg aggtctcctg gaacgacgga cgccgggtca cgaccgcgtt cgccgagcgg
     8521 cgcaccaccg gcgccacgtt cggctacctg gccgacggag ccgagtacgc gacgacgggc
     8581 tcgctggatc gggtcggcgc cgtcgtccgg atcctggtgc cccgccacga gatcggcgcg
     8641 gccgaggcga cccggatgaa ttcgatcgtg gcgcgcggca tgctcgtgac cggagcggtg
     8701 cgcgacgtcc gggacatcgc ccccgacatc caggcgtggt ccgtgcagta cgtggcgggc
     8761 gtgtcgtgcg tggatcaagc ccgcgcggcc tgcccggtcg cgctcgacgt caacggcgac
     8821 gccggcccgg tcgtggacca cgagggccgg accgtcccgg cctatcagcc ggcctcggat
     8881 ctgctggccg tgggcgcgag cgcgccggac ggaacgctcg tgctgtcggt ccgcgtcgcc
     8941 gacgcgggtg ccccgccgcc ggacggtttc acgatgcagg gctggacggt gtcgtggacg
     9001 tggcgcggca tccgctacat ggcgcaggcg gagcggaccc gcgaccgcgt ggtcttccgc
     9061 acccgcgcgc tcggcccggc cgacaagtac ggcaagccga ccggtccggc gttcatcggg
     9121 accgatgagg cgagcggatc gatcgacgcg caagcgggcg tgatccggat cgtgctgcgg
     9181 cgcgaatacg ccggttcgcc ctacgacggc gccgcgctca ccgatatggg cgcgctgtcg
     9241 tgggtgatga acgacggaaa ggtcgccgag ggcgcgtacg gaccggcccg ccacgtcacg
     9301 gtggatgaga cgccggtcgc cccgtaccgg atgggactgg gctgcagcgc ctagcggctc
     9361 cctgcgagcg cgacccgatc agccggtcgc gctcttgtcc ttgttgacgg cctcgaccga
     9421 cgcgcgcagc gcctccatca gatccacgac cggagcggct tcgccctcgt cggcgacgac
     9481 ggcgatctcc ttgccttcga tcttctgcga aaccagcgct tcgagcttct cgcggtaggt
     9541 gtcgcggaac tcgtcgggct tgaacacgtc ggtcatcgac tcaatcagca tccgcgccat
     9601 ctgcacctct tgatcgcgga cctgctgatc gtccttgagc tcttcgaagt cgggctcccg
     9661 gatctcgtcg ggccaataca tcgtctcgag cacgaagacg tcgtccttga cgcgcagcgt
     9721 cgccagatgc tccttgtcgc gccattgcac cttgaccagc gcgaccaggc cctcttcacc
     9781 gagcgccgtg cgcagcagcg cgtaggcctt cgtgccggtg gccccgtcgg gcgagaggta
     9841 gtacgaggac ttgaacagaa tcgggtcgat ctcttcctgg cggacgaact tgaggacttc
     9901 gatcgcgcgg ctgcccgaca ggccgcggtc cagctcgtct tcggtcagca cgacgtagtg
     9961 gtccttttcg tattcatagc ctttgacgat ctggtccgac gggacctctt cgccgtcgat
    10021 cgaacagacc cgcttgtatt tgattcgccc ctgatcgggc tcgtggagct ggttgaactt
    10081 cagcgacttt tcttccacgg ccgtgaagac ccggatcggg atcgtcacga gcccgaacga
    10141 gatcgcaccc ttccagatgg tccgcacgcg cctcacctct cgatttcgaa gatttcccgc
    10201 tcttctatcg tagtcccccg gcccacacga ggtcgatgga ttgccttgtt ggtaccctgc
    10261 gagcgatgac cggaatccgc cgtttcgcgt tcgccaccgg gctggtcgcg gtctgggtca
    10321 ccgagccgct ggattggagc gtcatcgcac cgacgcgcga ggcgtcgctg acgccgacga
    10381 cggcacatcc gcccaggacg gcacgcgacc ggctgatcgc acgcttcgtt cttcgccggc
    10441 gctaacggag gtcgtcggcg tcagcgacgc ctcgcgcgtc ggtgc
//
LOCUS       DATLFO010000010         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_119159, whole genome shotgun sequence.
ACCESSION   DATLFO010000010 DATLFO010000000
VERSION     DATLFO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119159"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VM841_00320"
     CDS             complement(<1..626)
                     /locus_tag="VM841_00320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88654.1"
                     /translation="MNSRYSRLVVLTVLISSLSGAVFAQDKPFEPQVGQPGKDVVWVP
                     TPQVLVEKMLDMAKVGPQDFVMDLGSGDGRTVITAAKRGARAVGIEYNPDMVALSQAN
                     AKKEGVTDRATFQRADIFETDFSKAQVITLFLLPSLNMKLRPTILSMRPGTRVVSNTF
                     TMEDWTPDETATVGDDCVSWCTALLWIVPAKAEGTWTVPGAGDLTLYQ"
     gene            770..1333
                     /locus_tag="VM841_00325"
     CDS             770..1333
                     /locus_tag="VM841_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HVL88655.1"
                     /translation="MASRPEFGQEVAIRVAMNSSPRLLALVLLSLFVNGCGQIGALGR
                     IVQPPTFSQADHQPAEIRLMGPSRTMPVGGAAVRIWLEVTNPNGFGFTLRTLNATLSL
                     EGSHAATGDFPLGLPLGARQESIVPLDLSISFVDIPGLARVLQQVAVGGVVDYELDGT
                     VGIDAGPFGTPTFGPMRLVTGELRLVR"
     gene            1426..2025
                     /locus_tag="VM841_00330"
     CDS             1426..2025
                     /locus_tag="VM841_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL88656.1"
                     /translation="MTFLSVDRVTTAAEIQGRAFFDDPLFAYVFPDLSERRNALPWLM
                     GVGLRYGISFGEVHTNAGEMRAHAVWLPPGRTDVDQDGMHVSGFGDAPSVMSAASITR
                     FGAFLEQVGPVHAQLMPDPHWYLMILGVDPPHQGKGIGGAMMQSVLARADADRLPCYL
                     ETAQERNVGFYRRFGFEVVNEHWIGDDGPRVWMMTRRPR"
     gene            2212..2571
                     /locus_tag="VM841_00335"
     CDS             2212..2571
                     /locus_tag="VM841_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88657.1"
                     /translation="MEPRGSLLAGLGLGVGLSYFLDPGRGARRRAQLRDRMAHTAAVT
                     RRALGTTTRDAMHRTYGTLAAARHLAQRNTHVDDAVLAERVRAKLGRHVSHPHSVHVT
                     ASDGVVTQCPSGGWSAR"
BASE COUNT          442 a          866 c          849 g          481 t
ORIGIN      
        1 tgctgataca gcgtcaggtc gccagcgcca ggcacggtcc acgtgccttc ggccttcgcc
       61 ggcacaatcc agagcagcgc cgtgcaccag ctcacgcagt cgtcgccgac cgtcgccgtc
      121 tcgtccgggg tccagtcctc catcgtgaag gtgttcgaca cgacgcgcgt gcctggccgc
      181 atgctcaaaa tcgtcggccg cagtttcatg ttgagcgacg gcagcagaaa gagcgtgatc
      241 acctgcgcct tcgagaagtc cgtttcaaag atgtcggcgc gctggaacgt cgcccgatcg
      301 gtaacccctt ccttcttcgc gttggcctgg gacagcgcga ccatgtccgg gttgtattcg
      361 atgcccacgg cacgggcacc ccgcttggcg gcggtgatca ccgtccggcc gtcgccggac
      421 ccgaggtcca ttacgaagtc ctgcggcccg acctttgcca tgtcgagcat cttctcgacg
      481 agcacctgcg gcgtcggcac ccagacgaca tcctttccgg gctggccgac ctgcggctca
      541 aacggcttgt cctgagcgaa tacggccccg ctgagcgagc tgataagaac agtcagaacg
      601 accaaacgtg aatagcgtga attcatgcgt aatcctcaac cacacctacg acagcccttc
      661 gaccgcgatc tgctggccgg gtcattggat cggccagttc ccatgtggga aggacatacc
      721 accagggttt gcaggcggaa gtaggcgatt gtaaagggct taacctgaag tggcaagccg
      781 gcccgaattc ggtcaggagg ttgcaatcag ggtcgccatg aactcttccc ctcggctcct
      841 ggccctcgtc ctcctttcgc tcttcgtgaa cggctgcggc caaatcggcg cgctcggccg
      901 gattgtgcag ccgccgacgt tcagccaggc ggaccaccag ccggcggaaa ttcggctgat
      961 gggcccctcg cggacgatgc ccgttggcgg tgctgccgtg cggatctggc tggaggtgac
     1021 gaacccgaat ggattcggct tcacgctgcg gacgctgaac gcgacgctgt ccctcgaggg
     1081 cagccatgcc gccacgggcg acttccctct cggtttgccg ctcggcgcac gccaggaatc
     1141 aatcgtgccg ctcgatctct cgatcagttt cgttgacatc ccgggacttg cccgtgtgct
     1201 gcagcaggtt gctgtcggcg gtgtcgtcga ctacgaacta gatggcaccg tcggcatcga
     1261 cgcaggtcct ttcggcacgc ccaccttcgg gccgatgcgc ctcgtcaccg gggaactgag
     1321 gctggtgaga taaaggaaac cggaggaaga ggagttttct ctgtgttcct gccggtggta
     1381 cgatgcgcac ccccgtaagc gagtgcacat gtctgtcacc ttcgtatgac atttctttca
     1441 gtagaccgcg tcaccacggc cgccgagatc cagggccgcg cgttcttcga tgacccgctg
     1501 ttcgcgtacg tgttcccgga tctctccgaa cgtcgcaatg cgctgccgtg gctgatgggg
     1561 gtcgggctcc gctacggcat cagcttcggc gaggtgcaca ccaacgcggg cgagatgagg
     1621 gcccacgcgg tctggctgcc gccaggccgg accgacgtcg atcaggacgg gatgcacgtg
     1681 agcggcttcg gtgacgcacc gtcggttatg agcgcagcgt ccatcacgcg atttggcgca
     1741 ttcctcgagc aggtgggacc ggtccacgcg cagctgatgc cggatccgca ttggtacctc
     1801 atgattcttg gtgtagatcc gcctcaccag ggcaagggca tcggaggtgc catgatgcag
     1861 tccgtgctgg cgagggccga tgccgaccgg ctgccgtgtt acctggagac cgcgcaggag
     1921 cggaatgtgg gcttctaccg caggttcggg ttcgaggtcg tcaacgaaca ctggatcggc
     1981 gacgacggac cgcgcgtctg gatgatgacc cgacgaccac gctgaagcag cgcaccacat
     2041 cctcaggcgc agctctggag tactgcttgc atccctcggg tacccaaacc gctggaggtg
     2101 tgccgtggtc cagatttcac gcatcggttt tcgcgagcga cgccgcaccg tacacccggt
     2161 gaatcgtctc ctgactcagc cgccgcagac cgccgcgagg ggggagtgac catggaacct
     2221 cgtggctcgc tgcttgccgg actgggcctt ggcgtggggc tctcgtactt tctcgaccct
     2281 gggagaggcg cacgccgccg cgcgcagttg cgcgaccgga tggcgcacac cgccgcggtg
     2341 acgcgccgcg cgctcggcac taccacgcgg gacgccatgc accgcacgta cggcaccctg
     2401 gccgccgcgc ggcacctcgc gcagcggaac acccacgtgg acgatgcggt gctcgcggag
     2461 cgcgtccgag cgaagctggg tcgtcacgtg tcgcatccgc attcggtgca cgtgacagcc
     2521 tcggacggcg tcgtgacgca atgcccgtca ggcggttggt cggcacgttg atcgctgcgc
     2581 gcgccatcag tccggaaccg atcaacccgg cgacgatgcc gcgcatgtcg cggcgggc
//
LOCUS       DATLFO010000011         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_20264, whole genome shotgun sequence.
ACCESSION   DATLFO010000011 DATLFO010000000
VERSION     DATLFO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20264"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VM841_00340"
     CDS             complement(<1..471)
                     /locus_tag="VM841_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88658.1"
                     /translation="MIDGPDPGFSLLVLLMLWPLVVAFCLVPLRDIRLIHRVALIGAL
                     VELGMSIALFARFAAAPTAGFQFREQLPWIDAIGASIHVGVDGLSAPFLPLTALLTVI
                     AVAQARTTVAHHQKPYLISVLTFSTASAGIFTALDLALFFVFWELMLIPTFVLIK"
     gene            complement(468..2039)
                     /locus_tag="VM841_00345"
     CDS             complement(468..2039)
                     /locus_tag="VM841_00345"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVL88659.1"
                     /translation="MPDELLAVDQVGFPVLTLLLLFPVVAAAIVSFLRDSRIATRVAI
                     GAAALEFVGTLVVALAFEPGTSNLQFVERTGAFLGVSWHVGVDGVSVLFVPLTALLGL
                     MVIGYAEHNVPARRAEGDPPVVGTASGYSAAMLLLEATMLGAFISLDLVIFWIFFVAE
                     LIPSWFLITRWGTGPQRRDAARNYVGYVGAGAALMLIGIVLTGNAYADATGEGASYEL
                     GRLIAAGLPPSSQTWPFVLFALGLAAKAPLFPFHTWLPKVLDHGPVVGMSVFLVGVKL
                     GTYGFLRFVIPLFPDSAQEWLWVMAAAGAVSVIYGSFLALGQTNLRRLLAFASVAHMG
                     VVMLGLFSLNLDGFEGALLQMINLGIVGAGLFFIAGFLTARIGQPELGSLGGLVSSAP
                     LMTTAFLVVALAGIGLPGTNGFNGEHLVMLGGYQRSWWIAVIAGLGVLLTAAYSLSYF
                     MRGFMGAPHKTLSDRIYDLTHGERAVVVALAALIFWIGLGTSPFIHRMRPSLTWLEQR
                     VEDVANGVAMMEARP"
     gene            complement(2039..3619)
                     /locus_tag="VM841_00350"
     CDS             complement(2039..3619)
                     /locus_tag="VM841_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL88660.1"
                     /translation="MGAAGKSAQMPLGGWLARAMEGPTPSSALIHGATMVTAGVYLVV
                     RSAPIYERAPIALVAVGLVGAVTVLYGQLAGLAQTDIKGMLAASTNAHLGFMLLLCGL
                     GLYTISIFHLVAHAFYKTNLFLTAPSILHHLHGGPDPTAVAPPTDTARGPAALVGAAA
                     LVLIALPLVAGPLPLPDEWARGTIALGGLGAVAAFGLWFSANRVVRATFHDTAANTRR
                     TMGAVAIGLGLVAVAIGLGLVPGGIEGSWFAQLLAPTVPQSGAAVEAPLAATIVLLVA
                     LICLMFSGIAVPRFFDRFRAEAPARTGGRFTRKLYFAAANRAWLDEGADRATNALTRA
                     GAAVDRFERAVLDPITGALMPSYRPRPDMTWEARLHAVADAADPEERAERPGLEWLGT
                     SPRAAARRHGRRLQTDDTPTLGPSSVAVRLERKLTAQPGGLYGALTDAGSSFSKRTER
                     VVFQRGVESVFERLSLTLASITEAIENHIFQLGPGRVAQVGGRIRRALLLMEAFLGRP
                     VVAAVVALGCIVTVAAVR"
BASE COUNT          786 a         1173 c         1161 g          500 t
ORIGIN      
        1 ctttatcaat acgaaggtcg ggatcagcat caactcccag aacacgaaga agagcgcaag
       61 gtctaaagct gtgaagatcc ctgcggacgc ggtcgagaag gtcagcacgg agatgagata
      121 gggcttctgg tgatgggcaa ccgtcgtgcg cgcctgggcg accgcgatca ccgttaacaa
      181 cgccgtaagt ggcaggaatg gcgccgacag gccgtcgact ccgacatgga tgctggcgcc
      241 gatcgcgtct atccagggaa gctgctctcg gaactgaaag cctgccgtag gcgcggctgc
      301 aaaacgagca aacaatgcga tcgacatccc aagctcgacg agcgcgccga tcagcgcgac
      361 ccggtggatc aaccggatgt cgcggagtgg aacgaggcag aaagccacca cgagcggcca
      421 gagcatcagc aggacaagaa gtgagaaacc ggggtcgggt ccgtcgatca aggccgcgcc
      481 tccatcatgg cgacgccgtt cgcgacgtct tcaactcttt gttcgagcca ggtcaaagat
      541 ggacgcatcc gatggatgaa ggggctcgtt ccgagaccta tccagaagat gagagcggcc
      601 aaggccacga ccaccgctcg ctctccgtgc gtgaggtcgt agatgcggtc ggagagtgtc
      661 ttgtgagggg cgcccatgaa gccgcgcatg aagtacgaca gcgagtaggc ggcggtcaac
      721 agaacaccga ggccggcgat gaccgcgatc caccaactgc gctggtagcc gcccagcatc
      781 acgaggtgct cgccgttgaa tccattagtg ccgggaaggc cgatccccgc aagcgcgacc
      841 accaggaagg ccgtcgtcat gaggggagca gaggagacga gccctccgag ggaaccgagc
      901 tcaggctgac cgatgcgggc cgtcaggaac ccggcgatga agaacaaccc ggcgcccacg
      961 atcccgaggt tgatcatctg caacagtgcg ccctcgaacc catcgagatt caaagagaag
     1021 agcccgagca tcactacccc catgtgcgcc acgctcgcga acgccagcaa gcgcctcagg
     1081 ttcgtttggc ctagagcgag aaaagaaccg tagataacac tgaccgcacc ggccgcggcc
     1141 atgacccaga gccattcctg agccgaatcc gggaagagtg ggatgacgaa gcgaaggaat
     1201 ccgtaggttc ccagcttcac tccgaccaaa aacacgctca tccccaccac cggcccatgg
     1261 tcgaggactt tcgggagcca cgtatggaag ggaaagagcg gcgctttggc cgccagtccc
     1321 agcgcgaaga gcacgaacgg ccacgtttgc gacgaaggag gtaagcccgc agcgatcaac
     1381 cgtcccaact cgtatgaggc gccctcgccg gtggcgtctg cgtatgcgtt gccggtgagg
     1441 acgatcccga tcagcatcaa cgccgctccc gcaccgacgt agccgacgta gttgcgggcg
     1501 gcatcccggc gctgcggccc cgtcccccag cgcgtgatca ggaaccaact cgggatcagc
     1561 tcggctacga agaaaatcca gaagatgact agatcgagcg agatgaaggc ccccagcatc
     1621 gtcgcctcca acaacagcat cgccgcggag tagccggatg cggtccccac cacgggaggg
     1681 tccccctcgg cgcgccgcgc cgggacgttg tgttccgcgt agccaatcac catcaatccg
     1741 aggagcgcgg tgagcggcac gaacagaacg ctcaccccgt ccactccgac gtgccaggaa
     1801 acaccgagga aagcaccggt gcgttcgacg aactgcaggt ttgaagtgcc gggctcaaaa
     1861 gccagcgcga cgactaacgt tccgacaaac tccagagccg cggctccgat agcaacgcgt
     1921 gtcgcgatgc gagaatcacg caggaaagag acgatcgccg ccgcaaccac agggaacagg
     1981 agcaggaggg tcaggacggg aaatccgacc tgatcgacgg ccaacaactc gtccggcatc
     2041 agcggaccgc cgcaacggtg acgatgcagc cgagcgcgac gacggcggca acgaccggtc
     2101 tgccgaggaa cgcttccatc agcagcaagg cccgccggat gcgccccccg acctgagcga
     2161 cccgaccggg gcccagttgg aagatgtggt tctcgatcgc ttccgtgatc gaagcaagtg
     2221 tcagcgaaag ccgttcgaac acgctttcga ctcctcgctg aaacaccact cgctccgtgc
     2281 gcttgctgaa cgacgagcct gcatcggtga gtgcgccgta aagacctccg ggctgcgcag
     2341 tgagcttgcg ctccagacga acggcgacgg aactgggccc tagggtgggc gtgtcgtcgg
     2401 tctgcaaccg gcgcccatga cggcgcgccg cggcgcgggg cgatgttccg agccactcca
     2461 gtcccggccg ctccgcccgc tcctccgggt cggcggcgtc ggcgaccgcg tgcagccgag
     2521 cctcccaggt catgtccgga cgcggcctgt aggaaggcat caacgccccg gtaatggggt
     2581 cgagcaccgc gcgttcgaaa cgatcgacgg cggctcccgc gcgcgttagg gcgttcgtcg
     2641 ctcgatccgc tccttcatcg agccaggctc ggttggccgc cgcaaaatag agcttgcgtg
     2701 tgaaccgacc gcccgtccgg gccggagctt ctgcccggaa acggtcgaag aaccgcggca
     2761 ctgcgatacc cgaaaacatc agacagatca gcgcaaccag gaggacgatg gtcgcggcca
     2821 agggggcttc caccgcggct ccgctctgtg gaacggtcgg ggccagcaat tgtgcgaacc
     2881 aagaaccttc gattccgccc gggaccaatc caagcccgat cgcgaccgcg accaatccca
     2941 gcccgatcgc aacggcaccc attgttcgac gtgtgttggc ggcagtatcg tgaaaagtcg
     3001 cacgcacgac ccggttggcc gagaaccaca acccgaacgc agccaccgct cccaagccgc
     3061 cgagcgcgat ggtcccccgg gcccactcat ccggtagcgg cagaggtcct gcaaccaagg
     3121 gaagggctat cagcacaagt gccgccgcgc ctacgagggc cgcggggcca cgagcggtgt
     3181 cggtcggtgg ggccaccgcc gtggggtcgg gcccgccgtg caggtggtgc aagatcgaag
     3241 gagccgtgag gaacagattc gtcttgtaga aggcgtgcgc cacgagatgg aagatcgaga
     3301 tcgtgtatag cccgagcccg cacaggagca gcatgaaacc gagatgggcg ttcgtggacg
     3361 cggcgagcat tcccttgatg tcggtttggg cgaggcccgc gagctggccg tacagcacag
     3421 tcactgctcc gaccagaccg accgccacga gcgcgatggg cgctcgctcg tatatgggag
     3481 cactccgaac cacgagatag acaccggcgg tgaccatcgt tgccccgtgt atcaacgcgc
     3541 tggacggcgt ggggccttcc atggcgcgcg ccagccaccc tccgagcggc atctgggcgg
     3601 acttcccggc cgcgcccaac
//
LOCUS       DATLFO010000012        10632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_119169, whole genome shotgun sequence.
ACCESSION   DATLFO010000012 DATLFO010000000
VERSION     DATLFO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119169"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VM841_00355"
     CDS             <1..747
                     /locus_tag="VM841_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVL88661.1"
                     /translation="EHGDRLLGSIPAPAGEMPVVLAPGGGGVLFHEACGHGLEADLCH
                     KHASVYRDRKGEKIGSELVSGVDEGNVPGSWGSFAFDDEGTPSQRTMLFENGVLTGYM
                     TDRIRSRAMDLKQSGNGRRQSYAHLPIPRMTNSSILPGSDDPGEIVRQTKKGLYAKSF
                     GGGQVNTATGDFVFGLTEAYLIEGGELTTPVRGANLIGNGPEILARIDAVGSDFEVWW
                     GVCGKDGQGVPVTTGMPTVRISKITVGGTG"
     gene            744..2081
                     /locus_tag="VM841_00360"
     CDS             744..2081
                     /locus_tag="VM841_00360"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVL88662.1"
                     /translation="MSDLDALCRAALDAAKGDEDLEAYAEWGRKTDVKVYDGRVESLT
                     SAEARGLGVRIIDGGRVGYAYAADPDPDEVKAIVEMARANAALGSPDEASILPAPEPI
                     EPLDGIYLPQIAATPTDEKVALAMELERITRAADPRVIGVEEAQYGDSVSRVAIRSTR
                     GVAAEAERGDCWAVVSALAKQGDETQTGFSFELARAPQDLNLQRCGGEAAMRAARMLG
                     ARKPATERMPVLLDPYAASSFLGVLAAGLTAEAVLKGRSLFAGKEGATVAANVFTLVD
                     DGRELKGPGASPFDDEGVPTRRTPLIEAGVLQGFLHNTYTATRLGGQSTGNAGRAGFK
                     STPGVGPSNLFVEPGSLDQAGLLATAGRALLVQDLIGVHSGANPISGDFSVGVTGLIY
                     DGGADPVPIREAAIAGTILQILGAITHVGSDLRFFGSTGSPTLLIGEMTVAGS"
     gene            complement(2083..3537)
                     /locus_tag="VM841_00365"
     CDS             complement(2083..3537)
                     /locus_tag="VM841_00365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL88663.1"
                     /translation="MPGVLVAYATAFGMWFAFGWGGEAMRAPVAELALLPVGIVGVAL
                     AARAALRDAAMRRAWMLIGAALGSMLAGDAIRAVIDLTMSRDTFPSVADLFTLSFYPL
                     VMAGLVSMPMSQRRAGDALKIGLDSLTILIGGGMLVWHFVMRPFAVRRGGLEGALALT
                     YPAADLVLLFGIAAVLFRRPDGAARWTLKILASACALLVGADLAYGALSLRGVSGAGA
                     WPDGLRMGAQALFAIAACLPQESLRRRRPAEDTHHVKRAFSMLPYASVAMAYGLLIVV
                     ARPVASDPAGFLLIGAIALTAVVTARQITVMFENERLTRRLERLVRLDELTGLFTRRA
                     FMDLAQREWARAHRYSRPLSALAIDVDRFKEINDTYGHATGDEVLAAVARRCRLSLRV
                     TDVLGRLGGDEFVAVLSDTTTRDARALVERLRGLMSLPVETGHGVIEVTVSIGLADAN
                     GSPNLRAMMDRADAALYQAKQSGRDCVREAAVAS"
     gene            3755..4387
                     /locus_tag="VM841_00370"
     CDS             3755..4387
                     /locus_tag="VM841_00370"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVL88664.1"
                     /translation="MNDRMLLRGLDAVIARHTEFTGPSEDSTRERILDAAFACASRHG
                     LGRTTMADVAREARLSRQSLYRYFPSRHDLILALVLREEQTMIGIVRHAMEPHQDLQP
                     AMTAAFAATLRAMRAHPLLDKVLAAEPHELLPYLTTEANPVLELSQNVMLRITAERAP
                     HVPEPLARRFAETCARVFTSYAITPPSDDPDTVAATLAELFCEGLARRNV"
     gene            4384..5457
                     /locus_tag="VM841_00375"
     CDS             4384..5457
                     /locus_tag="VM841_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018731028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL88665.1"
                     /translation="MTTYYELPVNDPLWSIPGTFDTRFNWEYDDQRAQLVTLYGKGKS
                     KQWDSEKRLDWSIPVDPGSPENGPDYYIPIFGSPTWEKLSEKEKSRVRHHIGSWLNSQ
                     FLHGEQGALICTAKIVQAVPDIDSKFYAATQVMDEARHVETYSRYLREKIEMAYPINK
                     YLKVLLDQVLQDNRWDFTYLGMQIMIEGLALAAFSMIRDFSTEPLAKAINTYVMQDEA
                     RHVAFGRLALRDYYPQLTDAERAEREDFIVEASFLMYGRFLAEEVWRHLEFDLDEVLE
                     YTRNSEMMQTFRKMLFSRIVPIVRDIGLLGPRVTKAFEELGVIEFKDFDAEELSRADE
                     QIAEEMDMRRREIEDMVRLGSED"
     gene            complement(5498..6370)
                     /locus_tag="VM841_00380"
     CDS             complement(5498..6370)
                     /locus_tag="VM841_00380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVL88666.1"
                     /translation="MSVLHALILGLLQGATEFLPVSSSGHLVLFPFIMGWEEPTVAFD
                     VAVHIGTLISVLIVFWDRVKPLVRALVRRDDRNRHLLTLLFVGSIPAGVAGLAFASVF
                     ESAFGRPMFAAMLLGVTGYLLILGDRFTKQHEERVAEEAAAARVAAARAQPLPETKPL
                     KTEDTMESLDAIAVGVAQAAAILPGISRSGSTVVAGLKMGVSREAAVRFSFLLSIPAI
                     LGALIVQLPDLASQGASGDLPAILVGVAASAVSGVVAIRWFIRLFVARGLKPFAIYCF
                     LAMIAGLLAGLARG"
     gene            complement(6375..7172)
                     /locus_tag="VM841_00385"
     CDS             complement(6375..7172)
                     /locus_tag="VM841_00385"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HVL88667.1"
                     /translation="MTSSAEIGIFGGSGVYSLLDSVEQVELDTPYGSPSAPVTLGEIA
                     GRRVAFLPRHGRSHQFPPHKINYRANIWALKQLGVSRIVAPVAAGSLQPNVRRGDFVV
                     CDQLVDRTSGRAATFYDGPATTHVGFADPFCPELRGIAIKAARDAGITVHETGTIVVI
                     EGPRFSSRAESRWYSSAGWEVINMTQYPESVLAREQEICYVNISLITDYDVGVEGVPA
                     VNVEDVIKVFADNNDKLRRLLFDIVPAIPEGRSCPCASALRGAVVGH"
     gene            complement(7169..7504)
                     /locus_tag="VM841_00390"
     CDS             complement(7169..7504)
                     /locus_tag="VM841_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HVL88668.1"
                     /translation="MPTYEYACRSCKRHTEAVQSFTDPPLTECPHCGGELKRVFHPVG
                     IVLKGSGFYSTDNRSSSKTKLATGKDTSTDSAGESPATDTSSAKKAEPAAKESKPAKK
                     ESKKTETKK"
     gene            complement(7565..8128)
                     /locus_tag="VM841_00395"
     CDS             complement(7565..8128)
                     /locus_tag="VM841_00395"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HVL88669.1"
                     /translation="MDIQDQKRIVRRTALARRAKVTRSERESAGAALAARVLSLPEIA
                     AAAEVMAFASFGTEIPTQPMVHALLASARRVLMPFVDGARMRAAVVEAPDDLAPGSRG
                     IPEPLDPVAVDPGPDVAVLVPGVAFDEHGRRLGYGGGFYDSFLATTPALRIGVCFDCQ
                     IVAEVPVESHDLPVRVIVTERRVIRPG"
     gene            8198..9577
                     /locus_tag="VM841_00400"
     CDS             8198..9577
                     /locus_tag="VM841_00400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HVL88670.1"
                     /translation="MKTRPVRAAGVAAGFGERRLVLSSRARVAFAILIGTGVSLLAAT
                     FAASAGLAARLGLAAGGIVALVGSGVLARYESLRVRLERSLNELQASRDELQSSLGTL
                     GEALRSTHDLTSLLQVVLETATRAMHASSGAAYLLTNTRTEMTVKCAHQLDRTFAGRR
                     IRVGEGIAGRVAQTRAPMLIPSDITPPAPSDAEPEATTMIAVPLESASHLIGVLALYG
                     REHGQKFTTADLETLMSLARQAAVGIENVLLHQEAQRLSITDGLTGLWNRRYLQMRLA
                     QEVERSIRFRRPLSVVLLDVDFFKAINDRYGHQRGDAVLIELSQRVVRAVRGQVDTLA
                     RLGGEEFVLLLPETAAEGARKVCQKILEAVSSEPFGGEGEDQAWVTVSIGCATFPQHG
                     TTPQTLLRTADFAMYEAKARGRNRVVMADELDSSPSGGDPMLPDEPEEPEPALLEIDV
                     MAADPFPDL"
     gene            9710..10582
                     /gene="galU"
                     /locus_tag="VM841_00405"
     CDS             9710..10582
                     /gene="galU"
                     /locus_tag="VM841_00405"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HVL88671.1"
                     /translation="MPIRKAVIPAAGLGTRFLPATKAQPKEMLPVIDKPAIQYVVEEA
                     VRAGLDDILIITGRGKRSIEDHFDQNVELEVFLESKGKTDEVREMRAISDLADIHYVR
                     QKEALGLGHAVAMAERHVGGEPFAVLLGDDIIMDPLLEEMVAVHEREGRSCLALMEVS
                     RDEISSYGAVAPEPVGPNLVKVADIVEKPSPAEAPSNLAVIGRYVLTPEIFDALRKTE
                     PGHGGEIQLTDAIRSLVDTQAVYGYVFEGTRYDIGKKIDYLRAGVELAAEREDLAAEF
                     REFLAEFVVRKKLV"
BASE COUNT         1745 a         3614 c         3693 g         1580 t
ORIGIN      
        1 gagcatggcg atcgcctgct cggctcgatc cccgcgccgg ccggcgagat gccggtggtg
       61 ctggcgcccg gcggcggcgg cgtgctgttc cacgaggcct gcggccacgg cctggaggcc
      121 gatctttgcc acaagcacgc cagcgtctac cgcgaccgca aaggcgagaa gatcggcagc
      181 gagctggtca gcggcgtcga cgaaggaaac gtgcccggct cgtggggctc gttcgcgttc
      241 gacgacgaag gcacgccctc gcagcgcacg atgctgttcg agaacggcgt gctgaccgga
      301 tacatgaccg accggatccg ctcgcgcgcg atggatctga agcagtcggg caacggccgg
      361 cgccagtcct acgcgcacct gccgatcccg cgcatgacca actcctcgat cctgccgggc
      421 agcgacgacc cgggcgagat cgtgcgtcag accaagaagg gcctgtacgc gaaatcgttc
      481 ggcgggggac aggtcaacac cgcgaccggc gatttcgtgt tcggcctcac cgaggcctac
      541 ctgatcgaag gcggcgagct gaccacgccg gtgcgcggcg cgaacctgat cggcaacggc
      601 cccgagatcc tggcgcgcat cgacgcggtc ggcagcgact tcgaggtctg gtggggtgtg
      661 tgcggcaagg acgggcaggg cgtgccggtc accaccggga tgccgaccgt ccgcatcagc
      721 aagatcaccg tcggaggaac cggatgagcg atctcgacgc gctgtgtcgc gcggcgctgg
      781 acgccgcaaa gggcgacgag gacctcgagg cgtacgccga gtggggccgc aagaccgacg
      841 tcaaggtcta cgacggccgg gtcgagtcgc tcaccagtgc cgaggctcgc gggctgggcg
      901 tgcgcatcat cgacggcggc cgtgtcggct acgcctacgc cgccgacccc gatcccgacg
      961 aggtcaaggc gatcgtcgag atggcgcgcg cgaacgcggc gctcggctcg cccgacgaag
     1021 ccagcatcct tcccgcgccc gagccgatcg agccgctcga cggcatctat cttccgcaga
     1081 tcgccgccac gccaaccgac gagaaggtgg cgctggcgat ggagctcgag cgcatcaccc
     1141 gcgcggccga cccgcgcgtg atcggcgtcg aggaggcgca gtacggcgat tccgtgtcgc
     1201 gcgtggcgat ccgctcgacc cgcggcgtcg ccgccgaggc cgagcgcggc gactgttggg
     1261 cggtggtgtc ggcgctggcc aagcaaggcg acgagaccca gacgggcttt tcgttcgagc
     1321 tggcgcgcgc gccccaggac ctgaacctgc agcgttgcgg cggcgaggcc gcgatgcgcg
     1381 cggcgcgcat gctcggcgcg cgcaagcccg cgaccgagcg catgccggtc ctgctggacc
     1441 cctacgccgc gtcctcgttc ttgggcgtgc tggccgccgg gctcaccgcc gaggcggtgc
     1501 tcaaaggccg gagcctgttc gcgggcaagg aaggcgcaac cgtcgccgcg aacgtgttca
     1561 cgctcgtcga cgacggacgc gagctcaaag gcccgggcgc gtccccgttc gacgacgaag
     1621 gagtgcccac gcgccgcacg ccgctgatcg aggcgggcgt gctgcagggc ttcttgcaca
     1681 acacctacac cgcgacccgt ttgggcgggc aatccaccgg caacgcgggg cgcgcgggct
     1741 tcaagtccac gccgggcgtg ggtccgtcga acctgttcgt cgaacccgga tcgctcgacc
     1801 aggcagggct gctcgccacg gcgggccggg cgctgctcgt gcaggacctg atcggtgtgc
     1861 attcgggcgc gaacccgatc tcgggcgact tcagcgtcgg ggtgacgggc ctgatctacg
     1921 acggcggcgc cgacccggtc ccgatccgcg aggccgcgat cgccggcacg atccttcaga
     1981 tcctgggagc gatcacccac gtcggcagcg acctccgctt cttcggaagc accggctcgc
     2041 cgacgctgct catcggcgag atgacggtcg ccggctcgtg atctagctcg cgaccgcggc
     2101 ttcacggacg cagtcgcggc cgctctgctt cgcttgatac agcgccgcgt ccgcgcggtc
     2161 catcatcgcg cgcaggttgg gcgacccgtt ggcgtccgcg agcccgatgc tcaccgtcac
     2221 ctcgatcacc ccgtgcccgg tttcgaccgg aaggctcatc aggccgcgga gcctctcgac
     2281 cagcgcccgc gcgtcgcgag tggtggtgtc ggagagcacc gccacgaact cgtcgccgcc
     2341 gagccggccg agcacgtcgg tgacgcgcag cgacaggcgg catcgccgcg cgacggcggc
     2401 gagcacctcg tccccggtgg catgcccgta ggtgtcgttg atctccttga agcggtccac
     2461 gtcgatggcg agcgcgctga gcggacgcga atagcggtgg gcccgcgccc actcgcgctg
     2521 ggccagatcc atgaacgcgc ggcgcgtgaa cagcccggtg agttcgtcga ggcgaaccag
     2581 ccgctcaagg cgacgggtga gccgctcgtt ttcgaacatg accgtgattt gccgggcggt
     2641 cacgaccgcg gtcagcgcga tcgcaccgat caggaggaag ccggccgggt ccgacgcgac
     2701 gggtcgcgcc acaacgatca agagtccgta ggccattgcc accgaggcgt acggaagcat
     2761 cgagaacgcc cgtttcacgt ggtgggtgtc ctcggccggg cgccgacggc gcagcgattc
     2821 ctggggcagg cacgccgcga tcgcgaagag cgcttgcgcg cccatgcgca ggccgtccgg
     2881 ccatgcgccc gccccggaca cgccccgcag cgagagcgcg ccgtaggcca gatcggctcc
     2941 cacgagcagc gcgcacgcgc tcgcgagaat cttcagcgtc catcgggcgg cgccgtcggg
     3001 ccgccggaac agcacggccg cgatgccgaa cagcagtacc agatcggcgg ccggatacgt
     3061 cagggcgagc gctccctcga ggccgccgcg cctcacggcg aaggggcgca tcacgaaatg
     3121 ccagaccagc atgccgccgc cgatcaggat cgtgagcgag tcgaggccga tcttgagcgc
     3181 gtcgccggca cggcgctggc tcatcggcat cgacaccagg ccggccatca ccagcgggta
     3241 gaacgacaac gtgaaaagat ccgcgacgga ggggaaggtg tcgcggctca tggtcaggtc
     3301 gatcacggct cggatcgcgt ccccggccag catggatccg agcgccgcgc cgatcagcat
     3361 ccaggcgcgg cgcatcgcgg cgtcgcgcag cgcggcgcgg gccgcgaggg cgacccccac
     3421 gatcccgacg ggcaggagcg ccagctcggc gacgggcgcg cgcattgcct cgccgcccca
     3481 gccgaaggcg aaccacatgc cgaaggcggt ggcataggcg accaatacac ccggcatcag
     3541 cctgggagca cgcaaggcaa gcgcccggaa tctcggcgcc tgcgtattcg acatgcctcg
     3601 gtggatcccg gcccgctcat caggcctgtt cgtccgcgac ggtcccaccc cctttgaggt
     3661 gaggtatcgg gctcgcggcc ttccggcttg aacgtccgga aggccggggg agacgctctt
     3721 gacaacatta cattttccgt cctagtgtca atccatgaac gatcgcatgc tgctgcgcgg
     3781 gctggacgcc gtcatcgcgc gtcataccga gttcacgggg ccctccgagg actccacccg
     3841 cgagcgcatc ctggacgcgg ccttcgcgtg cgcctcacgc cacggcctcg gccgcacgac
     3901 gatggccgac gtggcccgcg aggcccggct gagccgccaa agcctgtatc gctacttccc
     3961 gtcgcggcac gatctgatcc tggcgttggt gctgcgcgag gagcagacca tgatcgggat
     4021 cgtgcgccac gcgatggaac cgcaccagga cctgcagccg gcgatgaccg ccgcgttcgc
     4081 cgccacgctc agggcgatgc gcgcgcatcc gctgctggac aaggtcctgg ccgccgagcc
     4141 gcacgagctt ctgccgtacc taaccaccga ggccaacccg gtgctcgagc tgtcgcagaa
     4201 cgtgatgctc cggatcaccg ccgaacgcgc cccgcacgtg cccgagccgc tcgcgcgccg
     4261 cttcgccgaa acctgtgcgc gcgtgttcac ctcgtatgcc atcaccccgc cgtccgacga
     4321 tccggacacg gtcgccgcca cgctcgccga gctcttctgc gaagggctcg ccaggaggaa
     4381 cgtatgacca cctactacga gcttcccgtc aacgatccgc tgtggagcat tcccggaacg
     4441 ttcgacacgc ggttcaactg ggagtacgac gatcagcgcg cgcagctggt gacgctctac
     4501 ggcaagggca agtccaagca gtgggacagc gagaagcgcc tggactggtc gatcccggtg
     4561 gatccgggat cgcccgagaa cggccccgac tactacatcc cgatcttcgg ctcacccacg
     4621 tgggagaagc tttcggagaa ggaaaaatcc cgggttcgcc atcacatcgg ctcgtggctc
     4681 aacagccagt tcctgcacgg cgagcagggc gcgctgatct gcacggccaa gatcgtgcag
     4741 gccgtgcccg acatcgacag caagttctac gcggcgacgc aggtgatgga cgaggcacgt
     4801 cacgtcgaga cctactcgcg ctatctgcgc gagaagatcg agatggcgta tccgatcaac
     4861 aagtacctga aggtgctgct ggatcaggtc ctgcaggaca accgctggga cttcacctat
     4921 ctcggcatgc agatcatgat cgagggtctc gcgctcgcgg cgttcagcat gatccgcgac
     4981 ttctcgaccg agccgctcgc gaaggcgatc aacacctacg tgatgcagga cgaggcgcgc
     5041 cacgtcgcct tcggccggct cgcgctgcgc gactactacc cccaactgac cgacgccgag
     5101 cgcgccgagc gcgaagactt catcgtcgag gcttcgttcc tgatgtacgg ccgcttcctc
     5161 gccgaggagg tctggcgtca cctggagttc gatctggacg aggtgctcga gtacacgcgc
     5221 aactccgaga tgatgcagac gttccgcaag atgctcttca gccgcatcgt gccgatcgtg
     5281 cgcgacatcg gcctgctcgg cccgcgtgtg accaaggcgt tcgaagaact cggcgtgatc
     5341 gagttcaagg acttcgacgc cgaggagctt tcccgcgccg atgagcagat cgccgaggag
     5401 atggacatgc ggcggcgcga gatcgaggac atggtccgcc tgggaagcga agactgagct
     5461 tttcgtcgct gcaggcgatc cggccgccga tccggggtta gccgcgagcg aggccggcga
     5521 gcaggccggc gatcatggcc aggaagcagt agatcgcgaa cggcttgaga ccgcgcgcga
     5581 cgaacagccg gatgaaccac cggatcgcga ccacgccgct cacggccgag gccgcgacgc
     5641 cgaccaggat cgccggcagg tcgccgctcg ccccttggct cgcgagatcg ggcagctgca
     5701 cgatcagcgc gccgaggatc gccgggatcg acagcaagaa gctgaaccgc accgccgcct
     5761 cgcgcgacac gcccatcttc agcccggcga ccacggtcga gcccgaccgc gagatgcccg
     5821 gcaggatcgc ggcggcctgc gccacaccga ccgcgatcgc gtcgagggac tccatggtgt
     5881 cctcggtctt gagcggcttg gtctcgggca acggctgggc tcgggcggcg gccacgcgcg
     5941 cggccgcggc ctcctccgcg acccgttcct cgtgctgctt ggtgaaccgg tcacccagga
     6001 tcagcaggta gccggtcact cccaggagca tggccgcgaa catcgggcgc ccgaatgcgc
     6061 tttcgaacac gctcgcgaac gccaagccgg cgacgccggc gggaatcgag ccgacgaaca
     6121 gcagcgtgag caggtgccgg ttgcgatcgt cgcggcgcac cagcgcccgg acgagcggct
     6181 tgacgcgatc ccagaacacg atcagcacgc tgatcagcgt gccgatgtgg acggccacgt
     6241 cgaaggcgac ggtcggctcc tcccacccca tgatgaacgg gaacagcacc aggtggccgc
     6301 tggaggagac gggcaggaac tcggtcgcgc cctgaagcag ccccaggatc agcgcgtgca
     6361 ggaccgacat cgcctcagtg ccccacgacc gcgccgcgca acgcgctcgc gcacgggcag
     6421 gagcgccctt ccgggatggc gggcacgatg tcgaacagga gccgccgcag cttgtcgttg
     6481 ttgtcggcga agaccttgat cacgtcttcg acgttcacgg ccgggacccc ttccacaccg
     6541 acgtcgtagt cggtgatcag cgagatgttc acgtagcaaa tctcttgctc gcgggcgagc
     6601 accgattccg ggtactgcgt catgttgatg acctcccagc cggcgctcga ataccagcgg
     6661 gactcggcgc gcgaggagaa gcgcggccct tcgatcacca cgatggtccc ggtctcgtgc
     6721 accgtgatgc ccgcgtcgcg tgcggccttg atcgcgatgc cgcgcagctc ggggcagaac
     6781 ggatcggcga aaccgacgtg ggtggtcgcg ggcccgtcgt agaaggtcgc ggcgcggccg
     6841 ctggtccggt ccaccagctg gtcgcagact acgaagtcgc cgcgccggac gttaggctgc
     6901 agcgagcccg ccgccaccgg agcaacgatg cgcgacacgc ccagctgctt gagcgcccag
     6961 atgttggcgc ggtagttgat cttgtgcggc gggaactggt gcgagcggcc gtgcctcggc
     7021 aggaacgcga cgcgccggcc ggcgatctcg cccagcgtca cgggcgcgct gggcgagccg
     7081 tacggtgtgt cgagctcgac ctgttcgacc gagtcgagca gcgagtagac cccagagccg
     7141 ccgaagatgc cgatctcggc actggaggtc acttcttggt ctccgttttc ttggattcct
     7201 tcttggcggg cttggattcc ttggcggcgg gctcggcctt cttcgcggaa gaggtgtcgg
     7261 tggccgggct ttcaccggcc gagtccgtcg aggtgtcctt tccggtcgcc agcttggtct
     7321 tcgaggatga gcggttgtcg gtcgagtaga agccagagcc tttcagcacg atcccgaccg
     7381 gatggaagac gcgcttgagc tcgccgccgc agtgcgggca ttccgtcagc ggcgggtcgg
     7441 tgaagctctg gactgcttcg gtgtgccgct tgcaggagcg gcaggcgtat tcgtaggtcg
     7501 gcatagcagg cgggattgta tcggggaggc ggccgggtgc tcacgccgcc cggctcagcc
     7561 cggctcagcc cggccggatc acccggcgct cggtcacgat cacgcgaacg ggcaggtcgt
     7621 gggactcgac cggaacctcc gccacgatct ggcagtcgaa gcacacgccg atgcgaagcg
     7681 cgggggtggt ggccaggaac gaatcgtaga agccgcctcc gtacccgagc cgccgcccgt
     7741 gctcgtcgaa ggcgacgccc ggcaccagca ccgccacgtc cggtcccgga tcgaccgcga
     7801 ccggatccag cggctcggga atgccccggg agccgggcgc cagatcgtcg ggcgcttcga
     7861 ccacggcggc ccgcatccgg gctccgtcga cgaagggcat caggacgcga cgagcggatg
     7921 ccagcagagc gtggaccata ggctgggtgg gtatctccgt gccgaaggac gcgaaggcca
     7981 tcacttcggc ggcggcggcg atttcgggga gcgaaaggac tcgagcggcc agtgccgcac
     8041 ccgcggattc gcgttccgat cgggtaactt ttgctcgcct cgctagggcg gttcgccgaa
     8101 caatgcgttt ttggtcctga atgtccactg ccggggtcct taactcattg gcccggaaag
     8161 cgtatgagat aaaggtcgtc gcggcatttg ccgagacttg aagacaaggc ccgtccgtgc
     8221 cgcgggggtg gcggcgggct tcggggaacg gaggttggtg ttgtcgagcc gagccagggt
     8281 cgccttcgcg atcctgatag gaaccggcgt gtcgctgctc gcggcgacct tcgccgcgtc
     8341 ggccggactg gccgcgcggc tcgggctcgc cgccggcggc atcgtcgcgc tggtcggatc
     8401 cggcgtgctg gcccgctacg aatcgctgcg ggtgcggctg gagcgttccc tcaacgaact
     8461 gcaggcatcc cgtgacgaac ttcaatcgag cctgggcacg ctgggcgagg cgctgcgctc
     8521 gacccacgac ctgaccagcc tcctgcaggt ggttctggag accgcgaccc gggccatgca
     8581 cgcctcgtcg ggcgctgcgt acctgctgac gaacacccgg accgagatga ccgtcaagtg
     8641 cgcccatcag ctcgaccgca ccttcgcggg gcgccggatc cgggtcggcg agggaatcgc
     8701 tggccgggtc gcccagacgc gcgcgccgat gctgatcccc tccgacatca ccccgcccgc
     8761 gccgtcggac gccgaacccg aggccaccac catgatcgcg gtgccgctgg agtcggcctc
     8821 gcatttgatc ggcgtgctcg ccctctacgg ccgcgagcac gggcagaagt tcaccacggc
     8881 cgacttggag acgctgatgt cgctggcccg ccaagccgcg gtcggcatcg agaacgtttt
     8941 gctgcatcag gaggcgcagc gcctgtcgat caccgacggg ctgaccgggc tgtggaaccg
     9001 ccggtacctt cagatgcggc tggcgcaaga ggtcgagcgc tcgatccggt tccggcgtcc
     9061 gctgtcggtc gtgctgctcg acgtggactt cttcaaggcg atcaacgacc gatacggtca
     9121 tcagcgcggg gacgcggtgc tgatcgagct gtcgcagcgg gtcgtgcgcg cggtgcgcgg
     9181 gcaggtcgac acgctggcgc ggctcggcgg cgaagagttc gtgctgctgc tccccgagac
     9241 cgcggccgag ggcgcccgga aggtctgtca gaagatcttg gaggccgtct cgtccgagcc
     9301 gttcggcggc gagggcgagg atcaggcgtg ggtgacggtc tcgatcggct gcgcgacgtt
     9361 cccgcagcac ggcacgacgc cgcagacact gctgcgcacc gccgacttcg cgatgtacga
     9421 ggcgaaggcg cgcggccgca accgcgtcgt gatggccgac gagctcgact cgtcgccgtc
     9481 gggcggggat ccgatgctgc ccgatgagcc cgaggagccg gagccggcgc tgctggagat
     9541 cgacgtcatg gccgccgatc cctttccgga cctgtagacc gccggcggtg tcgctcgccg
     9601 gtcccggggc cgtttccgga tccgtgagcc cgttctcgtc cgtaaactcg cgggcggcgg
     9661 ctcgctcgct tgcccgccgc gtcacgtgct tctacactcc gccgccaaga tgcccatcag
     9721 aaaagcggtc atccccgccg ccggcctcgg gacgcgattt cttcctgcga cgaaggcgca
     9781 gccgaaggag atgctgccgg taatcgacaa gccggcgatt cagtacgtcg tcgaggaggc
     9841 cgtgcgcgcg ggcctggatg acatcctgat catcaccggc cgcggaaagc gctcgatcga
     9901 ggatcacttc gatcagaacg tcgagctcga ggtcttcctg gaatcgaaag gcaagaccga
     9961 cgaggtccgc gagatgcgcg cgatcagcga tctggccgac atccactacg tgcggcagaa
    10021 agaagcgctc gggctggggc atgcggtggc gatggccgag cggcacgtag gcggcgagcc
    10081 gttcgcggtg ctgctgggcg acgacatcat tatggatccg ttgctcgagg agatggtggc
    10141 ggtccacgag cgcgaggggc ggagctgcct ggcgctgatg gaggtcagcc gggacgagat
    10201 ctcctcctac ggcgcggtcg cgccggagcc cgtcgggccg aacctggtga aggtcgccga
    10261 catcgtcgaa aagccctcgc cggcggaagc gccgtccaat ctcgcggtga tcggacgcta
    10321 cgtgctcacg cccgagatct tcgacgcgtt gcgcaagacc gagccgggac acggcggcga
    10381 gatccagctg actgatgcga tccgttcgct ggtggacacg caggcggtct acggatacgt
    10441 gttcgaagga acgcgctacg acatcggcaa gaagatcgac tatctgcgcg ccggcgtcga
    10501 gctggccgcg gagcgggagg atcttgcggc ggagttccgc gagttcctcg ccgagttcgt
    10561 cgtgcgcaag aagctcgtct agctcctcat tccggtccag gcatcatccc tctccttctc
    10621 tccaccttcg ag
//
LOCUS       DATLFO010000013         7797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_59726, whole genome shotgun sequence.
ACCESSION   DATLFO010000013 DATLFO010000000
VERSION     DATLFO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59726"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VM841_00410"
     CDS             complement(<1..398)
                     /locus_tag="VM841_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HVL88672.1"
                     /translation="MTAPGNGHGHASRTIIISDREHGLPYSKGLMAGALMTAGLSPAR
                     AYHAAQTIEDRLVETGRDAVSTGELRRLAYDVLKEEAGERYAEAYMKWQAVADLDRPL
                     VILIGGGTGVGKSTIATQLGARLGIVRIIST"
     gene            complement(434..1750)
                     /locus_tag="VM841_00415"
     CDS             complement(434..1750)
                     /locus_tag="VM841_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HVL88673.1"
                     /translation="MTRSVVLIDGEHYPPVTQQGVDAVRARGYEVVAAVFCGGGEKLS
                     RALELGEIPIVRGESALHALDEALSSYAPDVVVDLSDDPVVDHAQRMRLASIALARNV
                     AYEAPGFRMDPPPRPRVARVPSIAILGTGKRCGKTAVSAHVARTLKAAGLRPVIVAMG
                     RGGPDKPLVVRGDLHPPTAESLIALAEAGNHAASDVYEDAVVAGVPAIGARRAAAGPA
                     GQTAFDTVSDAVEAAHELEPDVILLEGSGTAIPPVHADATILVARDTDDLGAWPGSHR
                     LLLADLLVVRMTEGKVVPTEDSALTSPNRVPSSVVRVNVALRPTPLGPVEGRRIYAAT
                     TAPPETGDALRDHLERTYGANVIGVTHHLADRARLETDLRAVEGRYEILAVELKAAAI
                     DVAARVARQGGAEIVFVDNRPIGIGHALEDEIAKVEEKARQRFEDR"
     gene            complement(1753..2541)
                     /locus_tag="VM841_00420"
     CDS             complement(1753..2541)
                     /locus_tag="VM841_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL88674.1"
                     /translation="MAVTIAHVSDWHVGSVHFVPNLMDRVIRELNELDPDFVVCTGDF
                     TNDGFRQEYKTAAAYLQQIRPRVLSVPGNHDARNVGYVHFEELIGSRHWAFEHGGVKV
                     VGADSSEPDLNIGQIGRERYPWIAEQFDGDYELKIYVQHHHLLPIPGTGRERNMVYDA
                     GDLLEVLIRSGVKLVLTGHKHVPYVWRIENLYVTNAGTASSLRLRGHTRPCYNIVTVD
                     DGEVVIRRKYPFGPDEVIAHFNIRTGQEKFRELQTIVEEPEVAK"
     gene            complement(2545..4770)
                     /locus_tag="VM841_00425"
     CDS             complement(2545..4770)
                     /locus_tag="VM841_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HVL88675.1"
                     /translation="MPTESAPPIQAVGPDGLVFEVDPEADARFAAARRESIMPDDPSG
                     LLATKEGDMFLYTTPDGNLSGGGTGLYFRDTRFLSVYAMEIAGSEPVLLSSSAERASM
                     SYIDCTNENVWEGERIVIPQQTLNVRRTRVIADRLFERIRIKNYNPYSVKVAVQLTLG
                     ADFADIFEVRGMSRDKRGTLFKPRVDGKVVTLAYRGEDAAFRATRIDLGVEPARFEIG
                     AGTVVVWFELELGAHYTRVLHLTIEPIVGEERRPHVSFESALHELRRSYEDWERSCTS
                     IDTDNELFNRLLQRSIRDLRALLTDTGEGRYLAAGIPWYVAPFGRDSILTSHQILMVN
                     PDPARDTLRMLARLQGREVDPWRDEEPGKILHEIRSGELAAATRIPHTPYYGSVDSTP
                     LFLILLGTHYKWTADRGFLEEMLPHAERALTWIDEYGDFDGDGFVEYQKRSPLGLDNQ
                     GWKDSHDSVVHADGTLAKGPIALAEVQAYVYLAKQRTAMMFDDLGLTGRATALREQAA
                     ALRHRFDEAFWMEDERYYALALDGDKRQVRPVTTNPAHGLYCGIIPPERAADVAERLL
                     EPDMFSGWGVRTMSKSSVAYNPMSYHNGSVWPHDNAFIAAGLKRYGYHRKTNRLATSL
                     FDAAVFGQTMRLPELFCGFTRRTPNRPVSYPVACSPQAWAAGAPFLMLQAMLGLSARA
                     PENLLTINSPVLPSWLNTIELRNLRVGGSTISIAFKREGDMTGFTVLSKQGDLRVIME
                     E"
     gene            complement(4936..5811)
                     /gene="thrB"
                     /locus_tag="VM841_00430"
     CDS             complement(4936..5811)
                     /gene="thrB"
                     /locus_tag="VM841_00430"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HVL88676.1"
                     /translation="MSVTVRVPATIANLGPGFDCLGLAIAWHNRVTLAPAGTTTIAVT
                     GPGEDRIPRNDNNLVLRSVRAWEAAVGAAPGEWAIDIDNDAPYGRGFGSSAAAIVGGL
                     VAARAAIGGDANVLALAGEIEGHLDNVSAALLGGITVSGYAPGDAIRIEPPSRIVPLV
                     CVAAGRLSTKEARASLPAQVPFGDAVANASRAALLVAVLTADDPARLLAATEERLHQP
                     PRFRIAPETGALVAALRSEGIAAFLSGAGPSVAALVAADEAGDIRALAERAAPEGWDV
                     RIVPFEANGAAEAAP"
     gene            complement(5819..6877)
                     /gene="thrC"
                     /locus_tag="VM841_00435"
     CDS             complement(5819..6877)
                     /gene="thrC"
                     /locus_tag="VM841_00435"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVL88677.1"
                     /translation="MSVAHQWRGIIEEYRDSLPVTDVTPVVTLREGGTPLIHSKNLSD
                     RLGCEVHLKYEGANPTGSFKDRGMTLAISKAAEEGSKAVICASTGNTSASAAAYAAKA
                     GMDCIVLVPQGAIALGKLAQALVHGARLLQVEGNFDEALTMARDLVERHPVTLVNSVN
                     PYRIEGQKTGAFEVVDMLGRPPDYHVMPVGNAGNITAYWKGYRELRDAERITYLPRMF
                     GFQAAGAAPIVLGHPVEKPQTIATAIRIGKPASWEQAVEAASRSEGAIKSVTDREIIA
                     AYRLLAREGIFVEMASAATVAGMLQLAEEGTFAAGKTIVGILTGHGLKDPDWAIAGAP
                     QPVVVPPDADTAARELGL"
     gene            complement(6891..>7797)
                     /locus_tag="VM841_00440"
     CDS             complement(6891..>7797)
                     /locus_tag="VM841_00440"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVL88678.1"
                     /translation="YEAAVCGGIPLIHPMKESLAGERVHKVMGIVNGTTNFILTRMAE
                     EGLEFGEALAEAERLGYTELDPSADIEGFDAAAKCAILATIAFNARVVAGDVFREGIA
                     DITQRDIAFAKRLGYTVKLLAIAEEQEGEIAARVHPAMIPDTHPLASVRENLNAVFVE
                     AERVGELMFYGRGAGGDPTATSVVGDVCDAARNVVRGAPGIGCTCYLDKPVRSMDESY
                     SQYYMLLEVADQAGVLAGVADVFAKHNVSIRSVWQEGLGEGAHLVLITHRAQERDLQA
                     TLDSLGNLDAVTRIASVMRVEGGEA"
BASE COUNT         1199 a         2629 c         2709 g         1260 t
ORIGIN      
        1 gtcgagatga tgcgcacgat cccgagccgc gccccgagct gggtcgcgat cgtggacttg
       61 cccacgccgg ttccgccacc gatgaggatg accagcggac gatccaggtc ggcgacggcc
      121 tgccacttca tgtaggcctc ggcatagcgc tcgccggctt cctccttgag cacgtcgtag
      181 gccaggcgcc gcagctcgcc ggtcgagacg gcgtcgcggc cggtctccac gagccggtcc
      241 tcgatggtct gggccgcgtg ataggcgcgc gcaggcgaaa ggcccgcggt catcaacgcg
      301 ccggccatga gccctttcga gtacggcaag ccatgctccc ggtcgctgat gatgatcgtc
      361 cgggatgcgt ggccgtggcc gttgccggga gccgtcatag tgtgggtttt cccagggtgg
      421 gtcgtcgtcg aacttagcgg tcctcgaacc gttgccgcgc cttctcttcg actttcgcga
      481 tctcgtcttc gagcgcgtgc ccgatgccga tgggacgatt gtcgacgaac acgatctccg
      541 cgccgccctg ccgcgcaacg cgggccgcga catcgatcgc cgccgccttc agctctacgg
      601 caaggatctc gtaccggcct tccacggcgc gcagatccgt ctcgagcctg gcgcgatcgg
      661 cgagatgatg cgttacgccg atcacgttcg cgccgtaggt ccgctcgaga tgatcacgca
      721 gcgcgtcccc cgtctccgga ggagccgtcg tcgccgcgta gatccgccgc ccctctaccg
      781 gccccaacgg tgtcggccgc aacgcaacat tgacgcgaac gacggatgaa ggaacgcggt
      841 ttggggaagt gagcgcggaa tcctcggtcg gaacgacctt gccttcggtc attctaacca
      901 cgagcaggtc ggcgagcaat aggcggtgcg agcccggcca ggcccccaaa tcgtcggtgt
      961 cccgggcgac cagaatggtc gcgtcggcgt gaaccggcgg gatggcggtg ccggaccctt
     1021 ccagaaggat cacgtccggc tcgagctcgt gggcggcctc gacggcgtcg gatacggtgt
     1081 cgaaggcggt ctggcccgcc ggtcctgccg cggcgcggcg cgccccgatg gcgggaacgc
     1141 ccgctacgac ggcatcttcg tagacatcgc tggccgcgtg gtttccggcc tcggcgagcg
     1201 cgatgaggct ctcggcggtg ggcgggtgca ggtccccgcg cacgacgagc ggcttgtcgg
     1261 gcccgccgcg tcccatcgcg acgatgaccg gccgcagccc ggccgccttg agcgtgcgcg
     1321 cgacgtgggc cgacacggcc gtcttgccgc accgcttccc ggtgccgagg atcgcgatcg
     1381 aaggaacgcg cgccacgcgt ggccgcggcg gcgggtccat tcgaaagccg ggagcctcgt
     1441 aggcgacgtt gcgcgcaagc gcgatcgaag cgagacgcat ccgctgcgcg tgatcgacaa
     1501 ccggatcgtc ggacaggtcc accacgacgt cgggggcata ggacgagagc gcctcgtcga
     1561 gcgcgtgcag cgcggactcg ccgcgcacga tcgggatctc gccgagctcg agcgcgcggg
     1621 acagcttctc tcccccgccg cagaagacgg cggcgaccac ctcgtagcca cgtgcgcgaa
     1681 cggcgtcaac accttgttgg gtgaccggcg ggtaatgctc gccgtcgatc agaacgacgg
     1741 agcgcgtcat ccttatttcg ccacttcggg ctcttcgacg atcgtctgga gctcgcggaa
     1801 tttctcttgg ccggtgcgaa tattgaaatg cgcgataacc tcgtcgggcc cgaacggata
     1861 cttgcggcgg atgacgacct cgccgtcatc gacggtcacg atgttgtaac agggccgggt
     1921 gtggccacga aggcgaaggc tggatgcggt cccggcgttg gtgacgtaga ggttctcgat
     1981 gcgccacacg tacggcacgt gcttgtgccc ggtgagaacg agcttgacgc ccgagcggat
     2041 gagcacctca agcaggtcgc ccgcatcgta gaccatgttg cgctcgcggc cggtgcccgg
     2101 gatcggcagc aagtgatggt gctgcacgta gatcttgagc tcgtaatcac cgtcgaactg
     2161 ctcggcgatc caggggtagc gctcacggcc gatctgaccg atgttgaggt ccggctcgga
     2221 cgagtccgcg cccacgacct tcacgccgcc gtgctcgaac gcccaatgcc gggacccgat
     2281 cagctcttcg aagtggacgt agccgacgtt gcgcgcgtcg tggttgcccg gcaccgaaag
     2341 cacgcgaggc cggatctgct gcagataggc ggccgccgtc ttgtactcct ggcggaagcc
     2401 gtcgttggtg aagtcgccgg tgcaaacgac gaagtcggga tcgagctcgt tgagctcgcg
     2461 gatcacgcga tccatcaagt tcggcacgaa atgcaccgag ccgacgtgcc agtccgaaac
     2521 gtgcgcgatg gttacggcca cggtctactc ctccatgatg acgcggagat cgccctgctt
     2581 ggacaggacg gtgaatcccg tcatgtctcc ttcccgcttg aacgcgatcg atatcgtgga
     2641 gccgccgacg cgcaggtttc gcaactcgat cgtgttcagc cagctgggaa gcaccggcga
     2701 gttgatggta agcaagttct ccggcgcgcg ggccgacagg cccagcatcg cctgcagcat
     2761 caaaaagggc gccccggcgg cccacgcttg cggcgagcaa gcaaccggat acgacaccgg
     2821 ccggttgggc gtgcggcggg taaagccgca gaacagctcg ggcaaacgca tcgtctggcc
     2881 gaagacggcc gcgtcgaaca gcgaggtcgc cagccggttg gtctttcggt ggtagccata
     2941 gcgcttgagc ccggcggcga tgaaggcgtt gtcgtgcggc cagaccgacc cgttgtggta
     3001 gctcatcggg ttgtaggcca cgctggactt cgacatggtg cgcacgcccc agccgctgaa
     3061 catgtcgggc tcgagcagcc gctcggcgac gtcggcggcc cgctccgggg ggatgatccc
     3121 gcaatagagg ccgtgcgcgg ggttggtggt caccggccga acctgccgct tgtcgccgtc
     3181 gagggcgagc gcgtagtagc gctcgtcctc catccagaag gcctcatcga agcggtgacg
     3241 cagcgcggct gcttgctcgc gcaaggccgt ggcccgtccg gtcaggccga ggtcgtcgaa
     3301 catcatggcc gtgcgctgct tggcgagata gacgtaggcc tgaacctcgg cgagtgcgat
     3361 cggccccttc gcgagcgttc cgtcggcgtg cacgaccgag tcgtgcgagt ccttccagcc
     3421 ttggttgtcc aggccgagcg gcgaccgctt ctggtattcg acgaagccgt cgccgtcgaa
     3481 gtcaccgtac tcgtcgatcc acgtgagcgc gcgctcggcg tgcggcagca tctcttcgag
     3541 gaagccgcgg tcggccgtcc acttgtagtg ggtgccgagc aggatcagaa agagcggtgt
     3601 ggagtccaca ctgccgtagt agggggtgtg cggaatgcgg gtcgcggcgg cgagctcgcc
     3661 gctgcggatc tcgtgcagga tcttgccggg ctcctcgtcg cgccagggat cgacctcccg
     3721 gccttggagc cgcgcgagca tccgcagggt atcccgggcg ggatccgggt tgaccatcag
     3781 gatctgatgg ctggtcagga tcgagtcgcg accgaaggga gcgacgtacc acgggatgcc
     3841 cgcggccagg taccgtccct cccccgtgtc ggtgagcagc gcgcgcaggt cgcggatgct
     3901 gcgctgcaga agccgattga acagctcgtt gtcggtgtcg atcgaggtgc aagaccgctc
     3961 ccagtcctcg taggagcgcc gcagctcgtg cagcgccgac tcgaacgaca cgtgcgggcg
     4021 gcgttcctcc cccacgatcg gctcgatcgt caggtgcagc acgcgcgtgt aatgcgcgcc
     4081 gagctcgagc tcgaaccaga ccaccacggt tcccgcgccg atctcgaagc gggcgggttc
     4141 gacgccaaga tcgatccgcg tcgccctgaa cgcggcatcc tcaccccggt aggcgagcgt
     4201 gacgaccttg ccgtccacgc gcggcttgaa caaggtgccg cgcttgtcgc ggctcatgcc
     4261 gcgaacctcg aagatgtcgg cgaagtcggc cccgagcgtc agctgcaccg cgaccttcac
     4321 cgaatagggg ttgtagttct tgatccggat ccgctcgaag agccggtcgg cgatcacccg
     4381 ggtgcgccgc acattgagcg tctgctgggg gatgacgatc cgctcccctt cccagacgtt
     4441 ttcgttcgtg cagtcgatat aggacatcga ggcccgctcg gcgctcgagg acagcagcac
     4501 cggctcggac ccggcaatct ccatcgcata caccgacagg aatcgcgtat cgcggaagta
     4561 caggccggtg ccgccgccgg acaggttccc gtccggcgtg gtgtagagga acatgtcccc
     4621 ttccttcgtg gcgagcaggc cggacgggtc gtcgggcatg atcgattcgc gccgggcggc
     4681 cgcgaatctg gcgtcggctt cgggatcgac ttcgaagacc aggccgtcgg gaccgacggc
     4741 ctgaatcggg ggcgcgctct ccgtgggcac ggccccagcg tattgcctcc cgggcctccg
     4801 gctcaatgtt cggcggccgc tcgcttgcaa tcgcacggtg tgccgggtca tcggcgggca
     4861 ccccgtgttc caatacggga cacgatccgg gcggcaacta cgacatctga tttgcggcgg
     4921 cgacgagcga cgcgatcacg gggcggcttc ggccgccccg ttcgcttcga acggcacgat
     4981 ccgcacatcc cagccctcgg gtgccgcgcg ctcggcgagg gcgcggatat cgccggcctc
     5041 gtcggcggcg accagggccg cgacgctcgg cccggcgccg gaaaggaacg ccgcgatgcc
     5101 ttcggagcgg agcgccgcga cgagggcacc ggtctcgggc gcgatccgga agcgcggcgg
     5161 ctggtggaga cgctcctcag tcgcggcgag cagccgagcg gggtcgtcgg cggtcagcac
     5221 ggccaccagc agcgcggcgc gcgaggcgtt ggcgaccgcg tcaccgaagg ggacctgcgc
     5281 cggcagagac gcgcgggctt ccttggtcga gagccgtccg gccgcgacgc acaccagcgg
     5341 cacgattcgc gacggcggct cgatccggat cgcgtcgccg ggcgcgtagc ccgacacggt
     5401 gatcccgccg agcagcgccg cgctgacgtt gtcgagatgc ccttcgatct cgcccgcgag
     5461 cgccagcacg ttcgcgtcgc cgccgatcgc agccctcgcc gcgaccaggc cgccgacgat
     5521 cgcggcggcg ctggagccga agccgcggcc gtagggcgcg tcgttgtcga tgtcaatcgc
     5581 ccactcgccg ggcgccgcgc ccaccgccgc ttcccacgca cgcaccgaac gcaggaccag
     5641 attgttgtcg ttgcggggaa tgcggtcctc gcccggcccg gtgacggcga tcgtggtggt
     5701 cccggcgggc gccagcgtga cgcggttgtg ccaggcgatc gcaagaccga ggcagtcgaa
     5761 gccggggccc aggttggcga tcgtggcggg aacgcgaacg gtgacgctca tggcggggct
     5821 agaggccgag ctcgcgcgcg gccgtgtccg cgtcgggcgg gacgacgacg ggctggggcg
     5881 cgcccgcgat cgcccagtcc ggatccttca agccgtgacc ggtcaggatg cccacgatcg
     5941 tcttgcccgc ggcgaaggtt ccttcctcgg caagctgcag catgcccgcg accgtggcgg
     6001 ccgatgccat ctcgacgaag atgccctcgc gggcgagcag gcggtaggcg gcgatgatct
     6061 cgcggtcggt gacggacttg atcgcgcctt cggaccggct cgcggcctcg acggcctgct
     6121 cccacgaggc gggcttgccg atacggatgg cggtcgcgat cgtctgcggt ttctcgaccg
     6181 gatggccgag cacgatcggc gcggcgcctg ccgcctggaa tccgaacatg cgcggcagat
     6241 aggtgatgcg ctcggcatcg cgaagctcgc gatagccctt ccagtaggcg gtgatgttgc
     6301 cggcgttgcc gaccggcatc acgtgatagt cgggcggacg gccgagcatg tccacgacct
     6361 cgaaggcgcc ggtcttttgt ccttcgatgc gatacgggtt cacgctgttg accagcgtca
     6421 ccggatggcg ctcgaccaga tcgcgcgcca tcgtgagcgc ctcgtcgaag ttgccctcga
     6481 cctgcagcag ccgcgcgccg tgcaccagcg cctgcgcgag cttgccgagc gcgatcgcgc
     6541 cttgcggcac cagcacgatg cagtccatcc cggctttggc cgcgtaggcg gcggcgctgg
     6601 ccgaggtatt cccggtcgag gcgcagatga cggccttgga cccttcctcg gcggccttgg
     6661 agatcgccag tgtcatgccg cgatccttga acgagccggt cggattggcg ccctcgtact
     6721 tgagatgaac ctcgcagccg agccggtcgg acaggttctt gctgtggatc aacggcgtgc
     6781 cgccttcgcg cagcgtgacg acgggcgtca cgtcggtgac cgggagcgag tcgcggtact
     6841 cctcgatgat gccgcgccac tggtgcgcga cgctcatcgc gatccgtcca tcaggcttcg
     6901 ccgccctcga cacgcatcac actggcgatg cgcgtgaccg catccagatt gccgagcgag
     6961 tcgagcgtgg cctgcaggtc acgctcctgc gcgcggtgcg tgatcagcac cagatgggcg
     7021 ccttcgccga gcccttcctg ccagacgctg cgaatcgaca cgttgtgctt ggcgaacacg
     7081 tcggcgacgc cggcgagcac accggcctga tccgcgacct cgagcagcat gtagtactgg
     7141 ctgtaggact cgtccatcga gcggaccggc ttgtcgagat aacaggtgca gccgatcccg
     7201 ggcgcgccgc gtaccacgtt gcgggccgcg tcgcacacgt cacccacgac gctggtcgcg
     7261 gtcggatccc cgcccgcgcc gcggccgtag aacatcagct ccccgacgcg ctcggcctcg
     7321 acgaagaccg cgttgaggtt ctcgcgcacc gacgcgagtg gatgcgtgtc ggggatcatc
     7381 gcggggtgca cgcgcgcggc gatctcgccc tcctgctcct ccgcgatcgc caggagcttg
     7441 accgtatacc cgagccgttt cgcgaacgcg atgtcgcgct gggtgatgtc ggcgatgccc
     7501 tcgcggaaga cgtcgccggc caccacgcgc gcgttgaacg cgatcgtcgc gaggatcgca
     7561 cacttggcgg cggcatcgaa gccctcgatg tccgcgctcg gatcgagctc ggtgtagccg
     7621 agccgctcgg cctcggcgag cgcttccccg aactcgagcc cctcctcggc catgcgcgtg
     7681 aggatgaagt tcgtcgtgcc gttcacgatc cccatcacct tgtgcacgcg ctcgccggcg
     7741 agggactcct tcatcgggtg gatcagcggg atgccgccgc acaccgccgc ctcgtag
//
LOCUS       DATLFO010000014         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_49862, whole genome shotgun sequence.
ACCESSION   DATLFO010000014 DATLFO010000000
VERSION     DATLFO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49862"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..300
                     /locus_tag="VM841_00445"
     CDS             103..300
                     /locus_tag="VM841_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88679.1"
                     /translation="MRETARESGRDPDAIEVTAGAMDVETMRALADAGVDRIVTPAFA
                     RTEEDLQRWFDDFEANVMSKL"
     gene            complement(317..1762)
                     /locus_tag="VM841_00450"
     CDS             complement(317..1762)
                     /locus_tag="VM841_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HVL88680.1"
                     /translation="MGERGGFLKHEREAIHYRPLEERVADWKEVYAPLPLANVKTQAA
                     RCMDCGVPFCHSGCPLGNLIPEWNDLAYRDDWRGAIERLHATNNFPEFTGRLCPAPCE
                     GSCVLGINAPAVSIKAIEVSIIDRAFDEGWVVPMPPSVKTGKAVAVIGSGPAGLAAAQ
                     QLTRAGHDVVVFERASKPGGLLRYGIPEFKMEKRLLDRRLAQMVAEGTHFRCDANVGE
                     DVPVDELRERFDAIVLCGGSTLPRDLPVPGRELAGIHFAMDYLTGANRVQEGSLDAPA
                     ITAEGKHVIIIGGGDTGADCLGTAHRQGAASVHQFEIMPQPPEERAPGNPWPQWPLIY
                     RTSSAHEEGGTRVYSVGTDRFEGDAAGNVTKLCGREVRMAVRDGRPAFEPVEGSEFEM
                     PCDLALLAMGFTGPQRTGMLERLGVEIDGRGNVVRDRDWKTTVDGVFVAGDMGRGQSL
                     IVWAIAEGRSAASAVDAYLMGETLLPAPLRP"
     gene            complement(1755..>3466)
                     /locus_tag="VM841_00455"
     CDS             complement(1755..>3466)
                     /locus_tag="VM841_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HVL88681.1"
                     /translation="YLAGADDLQIKIAQGAKPGEGGQLPGQKVYPWIAKTRFATPGVG
                     LISPPPHHDIYSIEDIAQLIHDLKNSNPSARVHVKLVAEVGVGTVAAGVCKAKSDVVL
                     ISGHDGGTGASPLSSVKHAGGPWELGLAETQQTLLLNGLRDRIVVQVDGQLKTGRDVV
                     IGALLGADEFGFATAPLVAIGCIMMRVCHLDLCPVGIATQNPELRKRFDGEPEHVMAF
                     MRFVAQEVREILAELGFRSLQEAIGRAEMIDTRAAIEHWKTDGMDLSPILHVPDVGDA
                     PRHHVGAQDHGLDKALDNVLIERCRDAIDEAKPVTVELPIRNVNRTVGTMLGHHVSKQ
                     WGEDGLPDGTIDIRFRGSAGQSFGAFLPRGIRLTLEGDANDYVGKGLSGGTVVLHPDR
                     DARFKAAENVIAGNVILYGATGGEAYIRGVVGERFCVRNSGATAVVEGVGDHACEYMT
                     GGRAVILGATGRNFGAGMSGGLAYVYDPAGTFSTRVNPEMVDLDPLDDEDEHWLRETV
                     EKHYRATMSGVAYVLLADWYKAAEQFVKVMPKDYKRVLEATRRAMERGESVEEAVMAA
                     SHG"
BASE COUNT          515 a         1221 c         1167 g          563 t
ORIGIN      
        1 tctcgcgcat gatgcgaaga agttcggcga gccggccggg ttcgggcatc cacggaaaga
       61 atccgtcacc catgcggccg gctcgccgaa cttcttcgca tcatgcgcga gacggcgagg
      121 gagtccggcc gcgacccgga cgccatcgag gtgaccgcgg gtgcgatgga cgtcgagacg
      181 atgcgggcgc tcgccgacgc cggcgtggac cgcatcgtca cgccggcctt cgcgcgcacc
      241 gaagaggatt tgcagcgctg gttcgacgac ttcgaggcga atgtgatgtc gaagctctag
      301 atctcgcagg attcgctcag ggccgcaggg gagcgggcag cagcgtctca cccatcaggt
      361 aggcgtccac ggccgatgcc gccgagcggc cctcggcgat ggcccacacg atcaggctct
      421 ggccgcgtcc catgtcgccg gcgacgaaca cgccgtccac ggttgtcttc caatcccggt
      481 cgcgaaccac gttgccgcgc ccgtcgatct cgacgccgag ccgttcgagc atgccggtgc
      541 gctgcggacc ggtgaagccc atcgccagca gcgccagatc gcagggcatc tcgaactcgc
      601 tgccctcgac cggctcgaat gccggccgtc cgtcgcgcac ggccatccgg acctcgcggc
      661 cgcacagctt ggtgacgttg ccggcggcgt cgccttcgaa gcggtcggtg ccgacgctgt
      721 agacacgggt gccgccctcc tcgtgcgcgg acgaggtgcg atagatcagc ggccactggg
      781 gccacgggtt gccgggggcc cgctcctcgg gtggctgcgg catgatctcg aactggtgca
      841 cggatgcggc tccttgccgg tgggcggttc ccaggcagtc cgcgccggtg tcgccaccgc
      901 cgatgatgat cacgtgcttt ccttcggccg tgatcgcagg agcgtcgaga gagccttcct
      961 gaacgcggtt cgcgccggtc aggtagtcca tcgcgaagtg gatgcccgcg agctcgcggc
     1021 ccggcaccgg aaggtcgcgc ggcagggtcg atccgccgca cagcacgatc gcgtcgaacc
     1081 gctcgcgcaa ctcgtccacc gggacgtcct cgccgacgtt ggcgtcgcac cggaagtgcg
     1141 tgccttcggc caccatctgc gcgagccgcc ggtccagcag ccgcttctcc atcttgaact
     1201 cggggatgcc gtagcgcagc aggccgccgg gcttggaggc gcgttcgaag acgacgacgt
     1261 cgtggccggc gcgggtgagc tgctgcgcgg ccgccagccc ggccgggccc gagccgatca
     1321 ccgcgacggc cttgccggtc ttgacgctcg gcggcatcgg cacgacccag ccctcgtcga
     1381 aggcgcggtc gatgatcgaa acctcgatcg ctttgatcga cacggccgga gcgttgatgc
     1441 cgagcacgca ggagccctcg cagggagccg gacacaggcg gccggtgaac tcgggaaagt
     1501 tgttggtcgc gtgaagccgc tcgatcgcgc cgcgccagtc gtcgcggtac gcaaggtcgt
     1561 tccactccgg gatgaggttg cccagcggac agccgctgtg gcagaaaggc acgccgcagt
     1621 ccatgcagcg cgcggcctgc gtcttgacgt tggcgagtgg cagcggcgcg tagacctctt
     1681 tccagtccgc gacgcgctcc tcgagcggcc ggtagtggat cgcctcccgc tcgtgcttga
     1741 ggaatccgcc gcgctcaccc atgggacgcc gccatgaccg cttcctccac cgactcgccg
     1801 cgctccatcg cgcgacgggt cgcctccagc actcgcttgt aatccttcgg catgaccttc
     1861 acgaactgct cggcggcctt gtaccagtcg gccagcagca cgtaggcaac gcccgacatc
     1921 gtcgcgcggt agtgcttctc gaccgtctcg cgcagccagt gctcgtcctc gtcgtcgagt
     1981 ggatccagat ccaccatctc ggggttgacg cgagtcgaaa aagtgccggc cggatcgtag
     2041 acgtaggcca ggccaccgga catgccggcg ccgaagttgc gcccggtcgc gcccaggatc
     2101 acggcccgtc cgccggtcat gtactcgcac gcgtggtcac cgacgccttc gaccacggcg
     2161 gtcgctcccg agttgcgcac gcagaaccgc tcgcccacga caccgcgaat gtaggcctcg
     2221 ccgccggtcg cgccgtacag gatcacgttg ccggcgatga cgttctcggc cgccttgaat
     2281 cgcgcatcac ggtcggggtg cagcaccacc gttccgccgg acagcccttt gccgacgtag
     2341 tcgttcgcgt cgccttccaa ggtcagccgg atgccgcgcg gcaggaacgc gccgaacgac
     2401 tggccggcgc ttccgcggaa ccggatgtcg atcgtgccgt cgggcaagcc gtcctcgccc
     2461 cattgcttcg acacgtgatg tccgagcatc gtgccgacgg tccgattgac gttcctgatc
     2521 ggaagctcga ccgtcaccgg cttcgcctcg tcgatcgcgt cgcggcagcg ctcgatcagg
     2581 acgttgtcga gcgccttgtc gagcccgtga tcctgtgccc cgacgtgatg gcgaggtgcg
     2641 tcgccgacgt cgggcacgtg caggatgggt gacaggtcca tgccgtcggt cttccagtgc
     2701 tcgatggcgg cgcgtgtgtc gatcatctcc gcccgcccga tcgcctcttg cagcgaccgg
     2761 aagccgagct ccgcgaggat ctcgcgcacc tcttgcgcga cgaaccgcat gaacgccatc
     2821 acgtgctcgg gctcgccgtc gaagcgcttg cgcagctcgg ggttctgggt ggcgatgccg
     2881 accggacaca ggtccaggtg gcacacacgc atcatgatgc agccgatcgc cacgagcggc
     2941 gcggtggcga agccgaactc gtcggcgccg agcagcgcgc cgatcaccac gtcgcggccg
     3001 gtcttgagct ggccgtccac ctgcaccacg attcggtccc gtaagccgtt gagcagcagg
     3061 gtctgctgcg tctcggccag gccgagctcc cacgggccgc cggcgtgctt gaccgacgac
     3121 agcggcgacg cgccggtgcc gccgtcatgg ccgctgatca gcaccacgtc cgatttggcc
     3181 ttgcacaccc cggccgcgac cgttcccacg ccgacctcgg cgacgagctt gacgtgcacg
     3241 cgcgcggacg ggttggagtt cttcaggtcg tggatgagct gcgcgatgtc ctcgatcgag
     3301 tagatgtcgt ggtgcggcgg cggcgagatc agcccaacgc ccggcgtcgc aaagcgcgtc
     3361 ttggcgatcc acggatacac cttctgtccc ggaagctgcc cgccttcacc gggcttggcg
     3421 ccctgcgcga tcttgatctg caggtcgtcg gcgccggcca ggtagc
//
LOCUS       DATLFO010000015        23177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_30213, whole genome shotgun sequence.
ACCESSION   DATLFO010000015 DATLFO010000000
VERSION     DATLFO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23177
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30213"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1119
                     /locus_tag="VM841_00460"
     CDS             151..1119
                     /locus_tag="VM841_00460"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HVL88682.1"
                     /translation="MDALAYGAIPPLDDPTHVPAAEIDPAGPEPLAVIEAGGRIRAYP
                     LSILIWHEAVNDSIGDVPVLVSYSPLTDSVTAWVRSAGEQVFSFAASGKVYAATGLLA
                     DRETGSLWLPPFGRAVAGSSKGLSLAGLPVAVVPASVYREAVPDGTVVSPETGFTRPY
                     GRSPYAGYLSRAEPPRRLFLREPDARLIPKAHVLAVPGDPVYPFEAVRAAGSVNDGGV
                     AVFWIERVASALDREVIAGARDTGSAVAYLSTLDGRRLTFDRMPGEPLRFRDAQTGTI
                     WNALGHGIEGPLRGRRLRPLAAVRSFWFAWAALDPPARVYGGGGEG"
     gene            1132..1728
                     /locus_tag="VM841_00465"
     CDS             1132..1728
                     /locus_tag="VM841_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL88683.1"
                     /translation="MAASPESFTETAIPLIEQMYGTALRLTRNPSDAEDLLQETSLKA
                     YQAFGQYESGTNLRAWLYKIMVNTFISSYRKSKRQPQTVPEGDNEDFSLFDTLAAATH
                     ETPEAVILDQIPDEQVKAALESLPDQFRMAVLLADVEGFSYKEIADITGVSIGTVMSR
                     LHRGRKQLQKRLWEFARTHGFVTEEEPWETPRRTADRS"
     gene            1692..1943
                     /gene="rsrA"
                     /locus_tag="VM841_00470"
     CDS             1692..1943
                     /gene="rsrA"
                     /locus_tag="VM841_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HVL88684.1"
                     /translation="MGNAETDCRQVLERLYLYIDGEIAGGDCASIEAHLKACPPCMQH
                     VDFEKNLKEVVRRKCTEGAAPPELAAKLKLQIQSMLGGS"
     gene            complement(1954..3219)
                     /locus_tag="VM841_00475"
     CDS             complement(1954..3219)
                     /locus_tag="VM841_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVL88685.1"
                     /translation="MQRPSDPAIWGDRALAAVAAVVAVVLIPFAQPAGFADALFTFGA
                     FVLLTELLDTELPSGKRLRLSLAPGLAFAMLEGTRAGSASAAVAGLTFLAAACVAGGI
                     RALAKREVRFGEIAASFLTVTAAAAAYDALAGTGPLHLTGRDGTHLASVTGMIAVLAI
                     ALAFEPARLWVTGAVDPVVRRARSPMALLRALRSNAALQSAIVSVAALVALAFPKLGG
                     WSFPLFLGPLAATRYAFRQITQIRTTEVQTLRALSKIPEMAGYTQPGHSRRVAELAVA
                     MAAELGAAEDELREIEYAALLHDIGRVVLEDPESPDAAAGLRADVATTGASIVAAAGT
                     FPEVARMIREQHDPYRRPGEILNDEITMGARILRVANTFDDLCTGNSANAKVSPWVAI
                     EALHGGAAYEYDPAVVSALARVLARDGVI"
     gene            complement(3203..4543)
                     /locus_tag="VM841_00480"
     CDS             complement(3203..4543)
                     /locus_tag="VM841_00480"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVL88686.1"
                     /translation="MKLSWPARLYIAAVVAAAAALLIATVRTGMPIPAGHAVLLASLF
                     LLAEAFPVALPRGGGYSVSFVVTLAAMITHGPAVAVIAAAAGALNLRGIRRHPAPVAS
                     RIFNGANFMVCTGIAALSYRAVGGPVGAELLAAPITKGLFPVLIATGVNFAANTAIVA
                     VMLYFATPQTRFRNTPWHIWRTEFTSLLPGYFSFAFLGLLLGVLYMEVHAAAVLFVLV
                     PLLVARRAFAAAVRAQLAYETTMESLVTALEAKDRYTRDHAARVSRLTEMVAREHGVR
                     GEQLRTFRLAALMHDVGKVGVPTAVLAKPGKLTPDEYVAMKDHPIRGHEIVTEIALLK
                     DALAGIRHHHERMDGSGYPDGLTGDEIPLVARIIMVCDAFDSMTSTRTYRVPMTMEEA
                     LAELRRCAGTQFDPAAVDALETALAKNPWRAEPEIERLRRLDEAKEKDNDAAAV"
     gene            complement(5265..5468)
                     /locus_tag="VM841_00485"
     CDS             complement(5265..5468)
                     /locus_tag="VM841_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL88687.1"
                     /translation="MTRDRFLRTSEVALLLQVSPKTVSRWAKEGRLPYMATLGGHRRF
                     PQSEIRRLTEDLNRSTEGAMFGG"
     gene            5621..6328
                     /locus_tag="VM841_00490"
     CDS             5621..6328
                     /locus_tag="VM841_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88688.1"
                     /translation="MSWENGTPGAGAPVASGEASNGEARPRAAAEIEAGLADIAGVRA
                     ARVVTGEDGHVAEVHVLASPGRPPKQLVRDIQSAVLTRFGITIDYRVVSVVQLEREVE
                     RQGPAPTAAAAAARPVLRRLSAETASFSTEIKVGLALEGTEIDRTTRGPATAGLRLVA
                     SATVDAVAEALSAEGVEVEFAAVVAAGPRQVALVVLRVLTGRGDQVVSGSAVIRRDPG
                     DAVARATLAALNRFVGS"
     gene            complement(6403..6729)
                     /locus_tag="VM841_00495"
     CDS             complement(6403..6729)
                     /locus_tag="VM841_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL88689.1"
                     /translation="MSHTTAGGSPLRTVAPDSDLAVRASDGTTVVIRSRARPPTFAAS
                     FTRRQSEILALVADGLSNAEIAQALGISRRTVEKHLETIHAKAGTATRARLVAFARNA
                     IRLDEP"
     gene            complement(6820..7368)
                     /locus_tag="VM841_00500"
     CDS             complement(6820..7368)
                     /locus_tag="VM841_00500"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HVL88690.1"
                     /translation="MLILLIILAAGIVLGRLRGGSLRRLGDAKLRMVQAVFAGVVLQG
                     SAAFVGSRTAATVLALTSHVGVFAFAGANAKRPGMPLLALGALMNFVVVAANGSMPVS
                     LDAMARAGIGSPAIGLPDLDALHEPLTDTTRLAFLADTIPVRVARHVVSPGDLVLWAG
                     LLAAIQGLMIARSRSGAGAHAA"
     gene            7462..8247
                     /locus_tag="VM841_00505"
     CDS             7462..8247
                     /locus_tag="VM841_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL88691.1"
                     /translation="MAEFATVSYSTEGPVARIAINRPDKRNALNVEVMRELRAAFAAA
                     KGDAAVRVAVLTGEGDKAFSAGGDLGGFTADQSKVEQHLLRGELARLFEDMRRLGKPV
                     VARVQGHALAGGFGLMCACDIVIASDAASFGMPEINMGLWPFIITVTVTRKIPPGKAL
                     ELMMTGERIDAGEAMRIGAVNKVVPAAGLDEAVDAMCATLAARSPIVMRLGKDSFYAA
                     EDMSLRDGLEYLHGQLGICLETEDVVEGVTAFFQKREPVWKGR"
     gene            8334..9974
                     /locus_tag="VM841_00510"
     CDS             8334..9974
                     /locus_tag="VM841_00510"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVL88692.1"
                     /translation="MSDETVASAGTAPGTAPEREMPTLTYQGPDMRGHTAELTERKKK
                     FAAMGGADKIARQHSLGKLTARERLDILFDDDTFEEFGLLAHHQSSSPQMQGKFTPAD
                     GVVTGVGKIDGRKTAVIAYDFTVMAGSMGMVGEFKAARMRELALRERMPIVWLIDSAG
                     ARIQEAAGSAFARTGELFREQVIMSGVIPQVAAMMGPGAAGTAYIPGLSDFVPMVKGV
                     SSMALGGPHLVKAAVGEEVTEQEMGGSQVHCKVSGVADLEVADDRECLDAVRRYLSYM
                     PSSNLEQPPAIEASDPVERRCEDLYDIVPANPRQAYDMRKVVKALADEGSFFPMKPDW
                     AKSMITGFARFGGRPVGIVANQPMFLGGAIDVNAADKAARFVWLCDAYHIPLVFLMDC
                     PGFLVGSAVEKQGIIRHGSKMLFAVSEATVPKVTVVLRKGYGAGYYVMNGRAYEPDLI
                     VGWPAAEISVMGPEGAVNIIGRSILEKIPEDQRDAQREAMIDAVRQQISPYVAAGWSF
                     VDDLIDPADTRRVICRGLERAATKRVERPWRKHGVLPV"
     gene            9971..10558
                     /locus_tag="VM841_00515"
     CDS             9971..10558
                     /locus_tag="VM841_00515"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HVL88693.1"
                     /translation="MRRALLGIGAMSLALSACGGGGDVESGPAQPREIEAADAAAGCG
                     PVVKFPEGSRQHVPQGTTVSYSTQPPVTGDHEGIWGATGTYAKEIPDEVQVHNLEHGH
                     VLIQYVPGEIDGPVLDGLVALARSNSEWILLAPRSADRFVPKASLAFTAWQVLRGCET
                     PSEKAVDAAKAFVAKYGKKAPERIPGDPIRETPPR"
     gene            10607..10903
                     /gene="clpS"
                     /locus_tag="VM841_00520"
     CDS             10607..10903
                     /gene="clpS"
                     /locus_tag="VM841_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HVL88694.1"
                     /translation="MSTQTAPVEVERPESDEAVRSDTPWIVIVWNDPINLMNYVVYVL
                     QKLFGYGKEKATKLMLQVHHEGRAIVSHGPREKCEADAARLHAHGLWATVSKDA"
     gene            10903..11388
                     /locus_tag="VM841_00525"
     CDS             10903..11388
                     /locus_tag="VM841_00525"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HVL88695.1"
                     /translation="MGPFARRRGGLELRLGEAEAEILAQVFRELRAQVAMEQPPPHLK
                     RLFPVAYPDDHEAQTEFASYTHDDLTAAKLSALEAVERAVSRGRVKRGLWTTTLDEEE
                     SGALLGALNDARLVLGTRLDVTEETEEAPVDESSPDAMALSVYRWLGWLQSYLVDQLL
                     A"
     gene            complement(11389..15249)
                     /locus_tag="VM841_00530"
     CDS             complement(11389..15249)
                     /locus_tag="VM841_00530"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL88696.1"
                     /translation="MRSRVRAGFVALAVAAALLAPMPATAAPLVVVQLTPANGSTVFP
                     PAEIRVRFNQCVDLPRSSLELRSPQGTLISVMVSYPDCRTIVIGPTETLGSGQFTIYA
                     FGVTIGGDSVSHTATFYSDGIAPVPPDPAMTHPINDSNRTKVYVTGTGEPGASVFVLI
                     DDDNGSTPAVACGVSGQPACGVVGSGGLFAVGPFNVTGLDDSPVPSTVVSTLSIEATV
                     TLTDPAGNVSAPRYAYAYKDVVYPAAPVVDWPTAGLQNANQRWTVTGTVSPQAGFEHM
                     DVLLYDNGVAATTARARCASGTCAWHGTIVSNPGSRTFVARARDAAGNVGPASASATV
                     TVTSSTSTGPIRPSIDIDPYPVRDALSASGVAQAGAPLEIKIGAAPYVACATASFSPG
                     TPNYSCGPFDTSAIAPGIFPVLVRDPSANGGTSGVNVLRDTVAPAAPMGRFSDPGPWV
                     NAQQAPGLRLDGSAEPFASVTGSVVSTGGGTSVVFTTQADVFGHWSATTNAALLNDGC
                     LSATIRAVDARGNTSAPSAIPCGPTLDKTAPPAPVIVVPAEGAIVTSTTFAVSGTAGG
                     GASTVQVFEGASLKATSTAAAFTGAGIAITVFNDGTKILRARSFDPAGNPSALSAPRT
                     IILDANAPVLSVTSPPNNGITNPSATITATYVDDDHANLGSCTLTVRDRGGALAMGVV
                     SLSGMSCIFSAASGALTVSGSPYTATVQASDPAGNQSQVDTWQFSIDDQPPAAPAFTA
                     PADGSLNGSPVEFRGTAEPGSTVQLFFGPTFLGASFANPTWSITASFSDGSYTVTAVA
                     IDAAGNVSPGANRSVVIDTTPPAAPVITAPASGATVGPGTAVTGTAEAGATVRLFDGP
                     VLLGQTVANPGWSITPSTSPEGPRTVTAFAVDPAGNTSAGTSRSYIVDTSSPGAPVIT
                     APAEGAFLAFTNVTVTGTAEVSSTVRIYEGAALLGTQAAAPGWSFPLTFSEGTHSVRV
                     TATDVAGNVSPDATRSFTVDTVAPGAPSILSPANGSLQPATFTLSGTAEPHATVQINE
                     GITLVATTTATSSGTWSASITASDGLHVYTARQTDRAGNIGPASAGRTVNVDATPPGA
                     PVISSPAEGSWQKRRVVIQGTAAEPGGLITVLENGQPIAAAQVSGTGAWEIVYDFPND
                     ATRGIVARQTDLAGNVGPNSPLRTFTTDGTPPAITITTANLYPILPVVSAANPTFTGS
                     AADQPGGGVMRVDVTILDVLGNVVQAAQAACSSCGPAGNPVAWTYTASSLLPGIYTIE
                     VTATDRAGNVTAPPERRTFVTLGL"
     gene            15497..15709
                     /locus_tag="VM841_00535"
     CDS             15497..15709
                     /locus_tag="VM841_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HVL88697.1"
                     /translation="MANVEAEMVANVWKVLVQPGATVAEGDTLVILESMKMEIPVEAP
                     HDGTVTAVNVHEGGVVQEGDVIAVIE"
     gene            15706..16494
                     /locus_tag="VM841_00540"
     CDS             15706..16494
                     /locus_tag="VM841_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL88698.1"
                     /translation="MSDLVEIVPGRVTLITLRRPGRRNALNTAMLDALREALAVADTD
                     EQARCIVLQGEGDHFCAGADFGDVTAGAEGGARYGAGFEALLRSIEEHRLPVVAGVQG
                     AALGAGCQLLAACDLAVAAEDARIGIPSARLGLLLDLEKIQRMAAVVGIPTVREMLLA
                     GREWTGVEAAERGLVTAAVPRSALAAAVAGLAERVASCAPLSVQGSKAGLRAIVDHGP
                     LDRMRDGAAFERHDERAIAAAQSEDLREGLAALRERRPPEFRGR"
     gene            complement(16491..17372)
                     /locus_tag="VM841_00545"
     CDS             complement(16491..17372)
                     /locus_tag="VM841_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HVL88699.1"
                     /translation="MASMSDGFRVGHWTDESVPTGCTVVLPPPGNVSSCDVRGMAPGS
                     RELAALAPHMPRSEVHAIVLSGGSAFGLAAADGAMRWCAERGAGFRAGPVVIPIVPAA
                     VVFDFAAAPGMRRPGPEEGYAACEAAIEGEVARGRVGAGTGASIGKWAGLDHRGPGGL
                     GVASAEQDDVRVCALAVVNAFGDIVGDDGTVLAGTRAARPSLRDVEAASWSENTVLAV
                     VTTTATIGKREAAFVAARGSDGITRAVRPAHTHYDGDVVFAVAAPGERPATRREVDLL
                     GMLAADAVASAVRDAVR"
     gene            17423..18856
                     /locus_tag="VM841_00550"
     CDS             17423..18856
                     /locus_tag="VM841_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVL88700.1"
                     /translation="MYPLRPLARRFTDLDEADVLHLQRLVADWQMLADLNFADLLLFV
                     PLRDEDGYIVLAQVRPFPAQTLYQEDMVGTVVPRRPRVARAIGEGRIVREGDPEWREG
                     VPIREEVIPVRHGDRVIGAISSEQNLSTARTPSKLELSYLQAAGDLAQMIADGEFPFP
                     GEQPENEIPLRVGDGFMRIDEAGVVTYVSPNAISAYRRLGVTANLVDEPLGGSEIEEE
                     SVLARLKQGRPVTSEVEANGAFVLRRFIPIVHEGRVEGALGFIRDTTELRRRDRMLVL
                     KDATIREIHHRVKNNLQTVASLLRMQTRRVGTAEGKQALEEAIRRISSIALVHETLSQ
                     DSSEMVEFDQVAQRVVDMVGDTLTGPDRPVRFSLSGSAGEIDADVATPLALVLNELCQ
                     NAVDHAFPHGGGKVEVTIERRGLNFRLTVADDGTGLPDGFSLEGGSNLGLQIVRTLVE
                     SELGGEITLSSDGGTRARVEIPLAQPS"
     gene            18853..19644
                     /locus_tag="VM841_00555"
     CDS             18853..19644
                     /locus_tag="VM841_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL88701.1"
                     /translation="MTLLLLHSAGATTALWDRVLEHLDGIPAFAWPLPGRAGAAWPDN
                     ERERDVAAYARAIMARMDGEGIERATIAGHSLGGAVALQMALAHSDRVAGLGLSCTGA
                     RLRVLPHVLEGFADGVRDVIDFWINVQCGPATGDRERIVMRRMLESAGMEQTWCDLRA
                     CDGFDVMDRLPEIGVRTQVFGGGHDVVTPPKYARYLAERISGARLTIFPGAGHTAPLE
                     HPEEMAGDLAVLWAATAPPVDASGRRRPSSALRPPATGRSDRSGC"
     gene            complement(19571..19774)
                     /locus_tag="VM841_00560"
     CDS             complement(19571..19774)
                     /locus_tag="VM841_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88702.1"
                     /translation="MREEYNGPVGFASEPAERRKSWIGRIITAGLLLFIIWLAVTRVI
                     STPTDPTFQSPGGGEPTTVVGGR"
     gene            complement(19830..20081)
                     /locus_tag="VM841_00565"
     CDS             complement(19830..20081)
                     /locus_tag="VM841_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVL88703.1"
                     /translation="MDWRHQASCRDVDPEIFFPIGTTGPALAQIEAAKAICAMCAVRE
                     PCLEWAIETAQDAGVWGGLTEEERRTLRRSRATRQRVAV"
     gene            20350..21048
                     /locus_tag="VM841_00570"
     CDS             20350..21048
                     /locus_tag="VM841_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HVL88704.1"
                     /translation="MKVIGHRGAAGRAPENTVAGVALAFADGADAVEVDVRLAADGTP
                     VIIHDETLDRTAGIAARVAALTSAELAGLIGGDRRIPTLAEVWDAASGRVVLELKGAW
                     GSGEAVEAARVVADFLASRDVSAAVVSCFDPAALAAFLDAGGTARTGVITLEALDPVS
                     NIRAAAEGGHHVCYIPGTLVTAEIVRLAHAAGKEIVAWTVNDPDVIRALAAAGADGVI
                     TDDPGAARAALAAR"
     gene            complement(21045..21962)
                     /locus_tag="VM841_00575"
     CDS             complement(21045..21962)
                     /locus_tag="VM841_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVL88705.1"
                     /translation="MKRALLIWNPVATTTTPGVRDVIARALSSELHVELAETTKRGHA
                     TDLAAEAAAADAFDLVCVLGGDGTINEAVNGLAGTGIPLATIPGGGTNVLARTLGYPK
                     DSVEATAMLLSRVREGLAPRTVNLGRVNGRAFAFCAGVGFDAEVVRRVEANPRAKRRF
                     GETFFISSGLREYFFPSSRPRPALELTGPDGKITDGIRAAIVGNSNPYTFLGSRPFRV
                     VPEARLDLGLDLTALRTMRITTVLRVIFSAFGPARHVRFRAVTTLHDADSFVLRADAP
                     ARFQVDGDLAGSDTEFRFSVERGALRVIG"
     gene            complement(21959..22192)
                     /locus_tag="VM841_00580"
     CDS             complement(21959..22192)
                     /locus_tag="VM841_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(22160..22162),aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HVL88706.1"
                     /translation="MKINIEYCAVUGYTDRAVGLAASLLGEMEQSVEIITIVPSGGGV
                     FEVTVDGDLVFSKKSLGRHAQPGEVEALVRGRG"
     gene            22344..22823
                     /locus_tag="VM841_00585"
     CDS             22344..22823
                     /locus_tag="VM841_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88707.1"
                     /translation="MRRSDVLDPAPPRHGTDGEGRGGWSWLVTAKDRFEAEVLRGMLE
                     NAGVPVILDGFDRSPFAWMYPAGNMNVPVQVLVPTGLLDVARLELMEAGLVGVDRLAP
                     ASAQAAAAAGPPASLLRDRTRPLWLVVAVLTALAVALFIIVEVAGFAPCATKVFCIK"
     gene            22889..>23177
                     /locus_tag="VM841_00590"
     CDS             22889..>23177
                     /locus_tag="VM841_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88708.1"
                     /translation="MIVPRSVSLALAVVLTAGVPASASQRQVTGRVEIPTFGSPALPR
                     TLYGATQSNGLVGWVLRVTPGRSFTLSTQAAPMGVEDFDIAFYASLDSSAPL"
BASE COUNT         3457 a         7901 c         8436 g         3383 t
ORIGIN      
        1 gtccaggtaa ccaaactctc cggggagggt caaatcacac gtttggcatt ccgtttgctc
       61 gggttgctgg cccttctggc cacctcatgc ggggggcccg ggttcccggg accgggcggg
      121 gctcggggcg agttcgaccc gagagaggtt gtggacgccc tcgcctacgg cgccatcccg
      181 ccgctcgacg atcccaccca cgtgccggcc gccgagatcg acccggctgg gccggagccg
      241 ctggcggtga tcgaggccgg cggccggatc cgggcgtatc cgctgtcgat cctgatctgg
      301 cacgaggccg ttaacgattc gatcggcgat gtcccggtct tggtctccta cagcccgctg
      361 accgactcgg tcaccgcctg ggtgcgctcg gccggcgagc aggtcttcag cttcgccgcc
      421 agcggcaagg tctacgcggc gaccgggctg ctcgccgacc gcgagaccgg atcgctatgg
      481 ctgcctcctt tcggccgggc ggtcgccggc tcgagcaaag ggctgtcgct cgcggggctg
      541 cccgtcgccg tggtcccggc atcggtgtac cgggaggcgg tgccggacgg aacggtcgtc
      601 tcgcccgaga cgggcttcac ccgcccgtac ggccgctcgc cctacgcggg ctacttgtcg
      661 cgcgccgagc cgccgcggcg tctgtttctt cgcgagcccg acgcgcgcct gatcccgaag
      721 gcccacgtgc tggccgtgcc gggcgatccc gtctacccgt tcgaggcggt gcgggccgca
      781 ggatccgtca acgacggcgg tgtcgcggtc ttttggatcg agcgggttgc gtcggcgctg
      841 gatcgcgagg tgatcgccgg cgcgcgtgac accggatccg ccgtggcgta cctgtcgacg
      901 ctcgacgggc gacgcctcac cttcgatcga atgccgggtg agccgcttcg cttccgcgac
      961 gcgcagaccg gcacgatctg gaacgcgctg ggccacggga tcgagggacc gctgcgtggc
     1021 cggcggctcc ggccgctcgc cgcggtgcgc agcttctggt tcgcgtgggc ggcgctcgac
     1081 ccgccggccc gggtttacgg tgggggtggg gagggctaag gtacccgcgc gatggccgcc
     1141 agccccgaat ccttcaccga gaccgcgatt ccgctcatcg agcagatgta cgggaccgcg
     1201 ctgcggctca cgcgcaaccc ctcggacgcg gaggatctgc ttcaagagac ctcgctgaag
     1261 gcctatcagg ccttcgggca gtacgagtcg ggcaccaacc tgcgcgcctg gctctacaag
     1321 atcatggtca acacgttcat ctcgtcctac cgcaagtcca agcgccagcc acagaccgtg
     1381 cccgaaggcg acaacgagga tttttccctg ttcgacacgc tcgccgcggc cacccacgag
     1441 acgcccgagg cggtcatcct cgaccagatc cccgacgagc aggtcaaggc cgcgctggag
     1501 tcgctgcccg accagttccg gatggcggtg ctgctggccg acgtcgaggg cttttcgtat
     1561 aaggagatcg cggacatcac gggcgtttcc atcggaaccg tgatgagccg gctccaccgt
     1621 ggacgcaagc aacttcagaa gcggctttgg gaatttgccc gtacccacgg gttcgttaca
     1681 gaggaagagc catgggaaac gccgagacgg actgccgaca ggtcttagag cgcctgtacc
     1741 tttacatcga cggcgagata gccggcggtg attgcgcgtc catcgaggcg cacctgaagg
     1801 catgcccgcc gtgcatgcag cacgtcgact tcgaaaagaa cctcaaagag gtcgtgcgcc
     1861 gcaagtgcac cgagggagcg gccccgcccg agctcgccgc gaagctgaag ctgcagatcc
     1921 agagcatgct cggcggaagc tagcggcgcc gcgctagatc acgccgtcgc gcgcgagcac
     1981 gcgggccagc gccgagacca cggccgggtc gtactcgtag gccgcgcccc cgtgcagcgc
     2041 ctcgatcgca acccacggtg agaccttcgc gttcgcggag ttgcccgtgc acaggtcgtc
     2101 gaaggtgttg gcgacgcgca ggatgcgcgc gcccatcgtg atctcgtcgt tgaggatctc
     2161 gccggggcgg cggtacgggt cgtgctgctc gcggatcatg cgcgcgactt cggggaaggt
     2221 gcctgcggct gcgacgatgc tcgcgcccgt ggtcgccacg tcggcccgaa ggccggccgc
     2281 ggcgtcgggg ctttcgggat cttcgagcac cacgcggccg atgtcgtgca gcagcgccgc
     2341 atactcgatc tcgcgaagct cgtcctcggc ggcgcccagt tcggcggcca tggcaacggc
     2401 cagctcggcg acgcggcgcg aatgacccgg ctgggtgtag ccggccatct cggggatctt
     2461 cgacagcgcg cgcagcgtct gcacctcggt cgtgcggatc tgggtgatct gacggaacgc
     2521 gtagcgcgtg gccgcgagcg ggccgaggaa cagcgggaag gaccacccgc cgagcttcgg
     2581 gaacgcgagc gcgacgagcg cggcgaccga cacgatcgcg gactgcagcg ccgcgttcga
     2641 acgcagcgcg cgcagcaggg ccatggggga gcgggcacgg cgtacgaccg gatcgaccgc
     2701 gccggtcacc cacagccggg ccggctcgaa cgcgagcgcg atcgccagca cggcgatcat
     2761 gccggtgacc gacgccagat gcgtgccgtc ccggccggtc aggtggagcg ggccggttcc
     2821 ggcgagcgcg tcgtaggcgg ccgcggccgc ggtcacggtc aaaaacgagg cggcgatctc
     2881 accgaagcga acctctcgct tggcgagcgc gcgaatgccg ccggcgacgc acgcggccgc
     2941 caggaaggtc aggcccgcga ccgcggcgga agcggagccg gctcgcgttc cctcgagcat
     3001 cgcgaacgcg aggccgggcg ccagcgacag gcgaaggcgc ttgccgctgg gaagctcggt
     3061 gtcgagcagc tcggtgagca ggacgaaggc gccgaaggtg aagagcgcgt cggcgaagcc
     3121 cgcaggctgc gcgaacggga tgagcaccac cgcgacgacg gccgcgacgg ccgccagcgc
     3181 gcggtcgccc cagatcgccg ggtcagacgg ccgctgcatc gttgtccttc tccttcgcct
     3241 cgtccaggcg ccggagccgt tcgatctcgg gctcggcgcg ccacgggttc ttggcaagcg
     3301 ccgtttccag ggcgtcgacg gccgcgggat cgaactgtgt gcccgcgcac cggcgaagct
     3361 cggccagcgc ctcctccatg gtcatcggca cccgataggt ccgtgttgag gtcatcgagt
     3421 cgaacgcgtc gcaaaccatg atgatccggg cgaccagcgg gatctcgtcg cccgtgagcc
     3481 cgtcggggta gcccgagccg tccatgcgtt cgtggtggtg gcggatcccg gcgagcgcgt
     3541 ccttgagcag cgcgatctcg gtgacgatct catggccgcg gatcggatgg tccttcatcg
     3601 cgacgtactc gtcgggcgtg agcttgcccg gcttggcgag cacggccgtg ggcaccccga
     3661 ccttgccgac gtcgtgcatc aaggcggcca gccggaaggt tcgaagctgc tcgccgcgca
     3721 cgccgtgctc gcgcgcgacc atctcggtca gccgggacac gcgcgcggcg tggtcgcggg
     3781 tgtagcggtc cttggcctcg agcgcggtga cgagcgactc catggtggtc tcgtaggcga
     3841 gctgggcgcg caccgcggcc gcgaacgcgc ggcgggccac cagcagcggc acgagcacga
     3901 agaggaccgc ggccgcgtgc acctccatat agaggacgcc cagcagcagc ccgaggaacg
     3961 cgaatgagaa gtagccgggc agcagcgagg tgaactcggt acgccagatg tgccagggcg
     4021 tgttgcggaa gcgcgtctgc ggcgtagcga agtagagcat gaccgcgacg atcgcggtat
     4081 tggccgcaaa gttgacgccg gtggcgatca gcaccgggaa caggcccttg gtgatcgggg
     4141 cggcgagcag ctcggcgccg acgggaccgc cgaccgcgcg gtacgaaagg gccgcgatcc
     4201 cggtgcagac catgaagttg gcgccgttga agatgcggct ggcgaccggg gcgggatggc
     4261 ggcggatgcc gcgcaggttc agcgcgccgg cggcggcggc gatgacggcg acggcggggc
     4321 cgtgggtgat catcgcggcg agcgtgacca cgaacgagac cgagtagccg ccgccgcggg
     4381 ggagcgcgac cgggaacgcc tcggccagaa ggaacaggct cgcgaggagt acggcgtgtc
     4441 cggccgggat cggcatgccg gtccggacgg tcgcgatcag caatgcggct gccgctgcga
     4501 cgacggcggc aatatagagt cgcgccggcc aactgagctt catcgctccg tttcctccaa
     4561 cgtcggggtt tcgatagggc tatgaggccg gcaggacccg cgggtgtgct gcgggtgatg
     4621 cggttacttc gggttcagcc agctcgcgtt gagccacgac gcgttcgcac ccgatgcaag
     4681 gaacacggtt gcgagcgcgg ccaagatgcc gagaatgcgc ttcttcattg ggtcgccccc
     4741 catcggacgg tagagatcgt tcttgatcga cccctcccgc ttccgctgtg gtgcctgcgc
     4801 gggaagtccg tccgctccgc tcgagtggcg agcccgtcgg gccctgccgg cctcatgtcg
     4861 ccctatacga ttttcaattg cgctttcgcg cccccaacgc gaggcgtgcg gaggatgcag
     4921 gtcccgcgcg tcccacccca gacgaccgct cgccgggcga atcttccgat tcgccgtcat
     4981 cggccgcaaa tgcaagaccc ccgaaacggc cgaaatcact catcggccgt gcggggggag
     5041 cacgtgtgcc tcatcggacg agcgatgctt tgtaacctct tttgagcatc cgggtcaata
     5101 gccacaccgg aaaacgatgg ctttcggaat ttgtttcgcc ttatccggaa ttccatgcgt
     5161 gtaatttcct ggtcgcctcg ggagcccggt ccccacgatg ggcaggcgcg cggcggcggc
     5221 cgggagcggc tcgcgccgtc cggccgccgg tgccgcaggg ggtctcagcc gccgaacatc
     5281 gctccctcgg tgctccggtt cagatcctcg gtcagccgcc ggatctcgct ttgcgggaag
     5341 cggcggtgac cgccgagggt ggccatatag ggcaggcgtc cctccttggc ccagcgcgag
     5401 acggtcttgg gagaaacctg cagcagcaaa gcgacttcgg acgtgcgcag gaatcgatcg
     5461 cgggtcacgg tgttctcctt tccgtgaggg gtcgtgagcc tcggctcgcg tgccggtggc
     5521 gacgccgcgg ccggtgcccg gctccggggc ctcggcgcat cgcccgggta gagtgggcca
     5581 tcacgccgga cccgctcggg ccggcttcgg agggatcccg atgtcgtggg aaaacggaac
     5641 gcccggcgcg ggcgccccgg tcgcgtccgg cgaggcgtcc aacggcgagg cgcgcccgcg
     5701 ggccgccgcc gagatcgagg cgggcctggc cgacatcgcc ggcgtgcgcg ccgcgcgggt
     5761 tgtgaccgga gaggacggcc atgtggcgga ggtccacgtt ctggcatcgc ccggccgtcc
     5821 gcccaagcaa ctcgtccgcg acattcagag cgccgtgctc acgcgcttcg gcatcaccat
     5881 cgactaccga gtcgtgagcg tcgttcagct cgagcgggag gtggagcggc agggtccggc
     5941 cccgacggcc gccgcagcgg ccgcccggcc ggtgttgcgg cgcctgtcgg ccgagacggc
     6001 ctcgttctcg accgagatca aggtcggcct cgcgctggag ggaaccgaga tcgaccgcac
     6061 gacgcgcggc cccgcgaccg cgggtcttcg cctggtcgcc tccgcgaccg tcgatgcggt
     6121 cgcggaggcg ctcagcgccg aaggggtcga ggtcgagttc gccgccgtcg tggccgccgg
     6181 tccccggcag gtcgcgctcg tggtgctgcg cgtgctgacc ggccgcggcg atcaggtcgt
     6241 gtccggttcg gcggtgatcc gccgcgaccc gggtgacgcg gtcgcacgcg ccacgctcgc
     6301 ggcgctcaac cgcttcgtcg gctcctagct tctgtcgtat cgcgcgcatg ccgcgcattg
     6361 tgcgagccgc gcgatgcgag cggcagggga gaagtacgta acttacggct cgtccaggcg
     6421 gatcgcgttg cgcgcgaacg cgacgagccg cgcgcgcgtc gcggttccgg ccttcgcgtg
     6481 gatcgtctcg aggtgctttt cgacggtgcg ccggctgatg cccagcgcct gtgcgatctc
     6541 ggcgttggac agaccgtccg cgaccagcgc caggatctcg ctttgccgcc gcgtgaatga
     6601 cgccgcgaac gtcggcggcc gcgcgcgcga ccgaatcacg accgtggttc cgtcggacgc
     6661 acgcaccgcc agatcgctgt ccggcgcgac ggtgcgaagc gggctccccc ccgctgtcgt
     6721 gtgagacatc gggaccctct cgatggcctc agagccgccg cccggcggac ccgtaccaga
     6781 gggaaacgac cgaccggggc cctaggacac gcggaagttt caggctgcgt gtgcgccggc
     6841 gccggaccgg ctccgtgcga tcatcagccc ttggatcgcg gccagcagcc cggcccatag
     6901 caccaggtcg cccggcgaga ccacgtgccg cgcgacgcga accgggatcg tgtcggccag
     6961 gaacgcaagg cgcgtagtgt cggtcagcgg ctcgtgcagc gcgtccagat ccggcagccc
     7021 gatcgcgggc gagccgatcc cggcgcgcgc catcgcgtcg agcgagaccg gcatcgagcc
     7081 gttagcggcg actacgacga agttcatcag cgcgccgagc gcgagcagcg gcatcccggg
     7141 ccgcttggcg ttcgcgccgg cgaaggcgaa cacgccgacg tgcgacgtga gcgcgagcac
     7201 cgtggccgcg gtccggctcc ccacgaacgc ggccgagccc tggagcacca cgccggcgaa
     7261 caccgcctgc accatgcgca gcttcgcgtc cccgagccgg cgcagcgaac cgccgcgcag
     7321 gcggccgagc acgatgccgg cggcgaggat gatcagaaga atcagcacgc gattggctcc
     7381 gaggggtgag ggccgggcta tccttcgcgc aatcatcgca cgcacgaagg ccgctcgctc
     7441 gcggtccgat ggagggggac gatggccgag ttcgcaacgg tttcgtattc gaccgagggc
     7501 ccggtggcgc gcatcgcgat caaccggccc gacaagcgca acgcgctcaa cgtcgaggtc
     7561 atgcgcgagc ttcgcgctgc attcgcggcc gccaagggcg atgccgcggt tcgcgtcgcc
     7621 gtgctcacag gcgaaggcga caaggcgttc agcgcgggcg gcgatctcgg cggcttcacc
     7681 gccgatcagt ccaaggtcga gcagcatctg ctgcgcggcg agcttgcgcg gctgttcgag
     7741 gacatgcgcc ggctcggcaa gccggtcgtg gcgcgcgtcc aagggcacgc gctcgcgggc
     7801 ggcttcggcc tgatgtgcgc gtgcgacatc gttatcgcct cggacgcggc gtccttcggc
     7861 atgcccgaga tcaacatggg cctgtggccg ttcatcatca ccgtgacggt cacgcgcaag
     7921 atcccgccgg gcaaagcgct cgagctgatg atgaccggcg agcggatcga cgcgggcgaa
     7981 gcgatgcgca tcggcgcggt caacaaggtc gttcccgcgg ccgggctcga cgaggcggtc
     8041 gatgcgatgt gcgcgacgct ggccgcacgc tcgccgatcg tgatgcgact cggcaaggac
     8101 tcgttctacg cggccgagga catgagcctg cgcgacgggc tcgagtacct gcacggccag
     8161 ctggggatct gcctggaaac cgaggatgtg gtcgagggcg tcaccgcctt cttccagaaa
     8221 cgcgagcccg tctggaaggg acgctaggcc ccgcgtacag tagcggctgc ccaggcgggc
     8281 tccggaaaac ttcggggatg ttccccggcg acgagccccc ggaagggaga accatgagcg
     8341 acgagaccgt tgcgtccgcc ggcaccgccc ccggcacggc gccggagcgc gagatgccga
     8401 cgctgaccta tcaagggccc gacatgcgcg gccacaccgc cgagctgacc gaacgcaaga
     8461 agaagttcgc ggcgatgggc ggggccgaca agatcgcgcg gcaacactcg ctcggcaagc
     8521 tcaccgcccg cgagcgactc gacatcctgt tcgacgacga caccttcgaa gagttcggct
     8581 tgctcgccca tcaccagtcc tcgtcgcccc agatgcaggg aaagttcacg cccgccgacg
     8641 gcgtcgtgac cggcgtgggc aagatcgacg ggcgaaagac cgccgtgatc gcctacgact
     8701 tcacggtcat ggccggatcg atgggaatgg tcggggagtt caaggccgca cgcatgcgcg
     8761 agctcgcgct gcgcgagcgc atgccgatcg tgtggctgat cgactcggcc ggcgcgcgca
     8821 tccaggaagc ggcgggaagc gcgttcgcgc gcaccggcga gctgtttcgc gagcaggtga
     8881 tcatgagcgg ggtcatcccg caggtcgccg caatgatggg tccgggcgcg gccggcaccg
     8941 cctacatccc cggcctgtcc gacttcgtgc ccatggtcaa gggcgtgtcg tcgatggcgc
     9001 tcggcgggcc gcatctggtc aaggccgccg tcggcgaaga ggtcaccgag caagagatgg
     9061 gcggctcgca ggtgcactgc aaggtgtcgg gtgtggccga cctggaggtc gccgacgatc
     9121 gcgagtgcct cgacgcggtg cggcgctacc tgtcgtacat gccctcgtcg aacctggagc
     9181 agccgccggc catcgaggcg tcggatccgg tggagcgccg ctgcgaagac ctctacgaca
     9241 tcgtgcccgc gaatccgcgg caggcctacg acatgcgcaa ggtcgtcaaa gccctcgccg
     9301 acgagggatc tttcttcccg atgaagcccg actgggccaa gtcgatgatc acgggttttg
     9361 cgcgattcgg cggccggccg gttggcatcg tggcgaacca gccgatgttt ctaggcggcg
     9421 cgatcgacgt caacgcggcc gacaaggccg cgcgtttcgt gtggctctgc gatgcctacc
     9481 acatcccgct ggtgttcctg atggactgcc cgggattcct cgtggggagc gcggtcgaga
     9541 agcagggcat catccgtcac ggctccaaga tgctcttcgc ggtgagcgag gcgaccgtgc
     9601 cgaaggtgac cgtggtcctg cgcaagggct acggtgccgg ctactacgtc atgaacggcc
     9661 gggcctacga gcccgacctg atcgtgggct ggcccgcggc cgagatcagc gtcatgggac
     9721 cggagggagc ggtgaacatc atcggacgat cgattctgga gaagatcccc gaagaccagc
     9781 gggacgcgca gcgcgaggcg atgatcgacg cggtgcgcca gcagatcagc ccgtacgtcg
     9841 cggccggctg gtcgttcgtg gacgacctga tcgatccggc ggatacgcgg cgcgtgatct
     9901 gccgcggcct ggagcgggcc gcgaccaagc gagtggagcg gccgtggcgc aagcacggcg
     9961 tgctgccggt atgaggcgcg ctctgctcgg aatcggagcg atgtcgctgg ccctgtcggc
    10021 ctgtggcggc ggcggtgatg tcgaaagcgg tcccgcgcag ccgcgcgaga tcgaggccgc
    10081 cgatgcggct gcaggctgcg ggcccgtggt gaagttcccg gaaggctccc gtcagcacgt
    10141 gccccaaggc accaccgtct cgtactcgac gcagccgccg gtcaccggcg accacgaggg
    10201 catctgggga gcgaccggaa cctacgccaa ggagatcccg gacgaggttc aggtccacaa
    10261 cctcgagcac gggcacgtgc tcatccagta cgtgccgggc gagatcgacg gtccggtgct
    10321 cgacgggctg gttgcgctcg cgcgatcgaa ctcggagtgg atcctgctcg cgcctcgatc
    10381 cgccgatcgg ttcgtgccga aagcgtcgct tgctttcacc gcgtggcagg tgcttcgcgg
    10441 atgcgagacg ccgagcgaga aggcggtcga tgcggcgaag gcgttcgtgg cgaagtacgg
    10501 caagaaggcg cccgagagga tcccggggga tccgattcgc gaaacgccgc cgcgatgatc
    10561 gcgcgatcgc gatgatcgcg cgacccgtga tcgctaaact ggcgccgtga gcacccaaac
    10621 cgccccggtc gaggtcgagc gacccgaatc cgacgaagcc gttcgttccg acacgccgtg
    10681 gatcgtgatc gtgtggaacg acccgatcaa cctgatgaac tacgtcgtgt acgtcctcca
    10741 aaagctgttc ggctacggca aggagaaggc cacgaagctc atgctgcagg tgcatcacga
    10801 aggcagggcg atcgtctcgc acggtccgcg cgagaagtgc gaggccgacg cggcgcggct
    10861 gcatgcgcac gggctctggg caaccgtgtc caaggacgcg tgatggggcc gttcgcgcgg
    10921 cggcgcggcg gcttggagct tcgcctcgga gaggccgagg ccgagatcct cgcccaggtg
    10981 ttccgcgagc ttcgcgcgca ggtcgcgatg gagcagccgc cgccgcacct caagcgcctg
    11041 ttccccgtcg cctatcccga cgaccacgaa gcccagaccg agttcgcctc ctacacccac
    11101 gacgacctga ccgcggcgaa gctgtcggcg ctcgaggcgg tcgagcgcgc cgtgtctcgc
    11161 ggccgcgtca agcgcgggct gtggaccacc acgctcgacg aggaagagtc tggcgcgctg
    11221 ctcggcgcgc tcaacgacgc gcggctcgtg ctcggaaccc gcctcgacgt caccgaggaa
    11281 accgaggagg cgccggtcga cgagtcctca cccgacgcga tggcgctcag cgtctaccgc
    11341 tggctcggct ggctccagtc ctatctggtc gatcagctgc tcgcctgact acaggccgag
    11401 ggtgacgaag gttcgccgtt ccggcggcgc ggtgacgttg ccggcccggt ccgtcgccgt
    11461 gacctcgatc gtgtagatgc ccggcagcag cgacgacgcc gtgtaggtcc acgccaccgg
    11521 gttgccggcc gggccgcacg acgagcacgc cgcctgtgcg gcctgcacca cgttgccgag
    11581 cacgtccaag atcgtcacgt ccacgcgcat cacgccgccc ccgggctgat cggcggccga
    11641 gccggtgaag gtcgggttcg cggccgacac caccggaagg atcggataca ggttcgcggt
    11701 cgtgatggtg atcgccggtg gcgtgccgtc ggtggtgaag gtgcgaagcg ggctgttcgg
    11761 tccgacgttg ccggccaggt cggtctgccg cgcgacgatg ccgcgggtcg cgtcgttggg
    11821 gaagtcgtag acgatctccc aggcgcccgt tccactgacc tgcgcggcgg cgatcggctg
    11881 tccgttctcc aggacggtga tcagcccgcc gggctcggcg gctgtgccct ggatcacgac
    11941 gcgccgcttc tgccaggagc cttccgcggg cgacgagatc accggcgcgc cgggcggggt
    12001 cgcgtcgacg ttgacggtgc gcccggcgga cgcggggccg atgttgccgg cgcggtccgt
    12061 ctggcgcgcc gtgtagacgt gcaggccgtc gctcgcggtg atcgacgcgg accaggtgcc
    12121 gctcgaggtt gccgtcgtgg tcgcgacgag cgtgatgcct tcgttgatct ggacggtcgc
    12181 gtgcggctcg gcggttccgc tgagcgtgaa cgtcgcgggc tgcaggctgc cgtttgcggg
    12241 cgacaggatc gacggcgcgc ccggcgcgac cgtgtcgacg gtgaacgagc gggtcgcgtc
    12301 gggcgagacg ttgcccgcca cgtcggtcgc cgtgacgcgc acgctgtggg tgccctcgct
    12361 gaacgtcagc gggaacgacc atccgggtgc ggcggcctgg gtgccgagca gcgccgcgcc
    12421 ttcgtagatg cgtactgtgc tcgagacttc ggccgtgccg gtgaccgtca cgttcgtaaa
    12481 ggccaggaac gcgccttccg caggcgcggt gatgaccggt gctccgggcg aggacgtatc
    12541 gacgatgtag ctgcgcgacg tgcccgcgct ggtgttgccg gccggatcga cggcgaaggc
    12601 ggttacggtg cgcggccctt ccggcgacgt ggacggcgtg atcgaccagc ccgggttcgc
    12661 gaccgtctgg ccgagcagga ccggcccgtc gaacaagcgc accgtcgcgc cggcctcggc
    12721 ggtgccggtg accgccgtgc ccggaccgac cgtcgcgccc gatgcgggtg cggtgatgac
    12781 cggcgccgcg ggcggggtgg tgtcgatcac gacgctacgg ttggcgcccg gcgagacgtt
    12841 gccggccgcg tcgatcgcga ccgcggtgac ggtgtagctg ccgtcggaga agctcgcggt
    12901 tatggaccag gttgggttgg cgaacgacgc gccgaggaag gtcggcccga agaacagttg
    12961 caccgtcgag ccgggctcgg ccgtgccccg gaactcaacg ggtgagccgt tgagcgagcc
    13021 gtcggccggc gcggtgaagg ccggcgcggc gggcggctga tcgtcgatcg agaactgcca
    13081 ggtgtccacc tgggactggt tgccggcggg gtcgctggcc tgcacggtcg cggtgtaggg
    13141 gctgccggaa accgtgagcg cgcccgaggc ggccgagaag atgcacgaca tgcccgacag
    13201 cgacaccacg cccatcgcga gtgcgccgcc ccggtcgcga acggtcagcg tgcacgagcc
    13261 gaggttcgcg tgatcgtcgt cgacgtaggt cgccgtgatc gtcgccgacg ggttggtgat
    13321 gccgttgttg ggcgggctgg tcaccgaaag gaccggcgcg ttcgcgtcca ggatgatcgt
    13381 gcgcggcgcc gacagcgccg acgggttgcc ggccgggtcg aacgagcgcg cgcgcaggat
    13441 cttggtgccg tcgttgaaga ccgtgatcgc gatgccggcg ccggtgaacg ccgccgccgt
    13501 gctcgtcgcc ttcagcgagg ccccttcgaa gacctgcacg gtcgaggcac cgccgccggc
    13561 cgttcccgac accgcgaagg tcgtcgaggt cacgatggcg ccttccgcgg gcacgacgat
    13621 taccggcgcg ggcggggcgg tcttgtccag cgtcggtccg cacggaatcg ccgacggcgc
    13681 cgacgtgttg ccacgcgcat cgacggctcg gatcgtcgcc gacaggcatc cgtcgttgag
    13741 cagcgctgcg ttcgtggttg cgctccagtg accgaacacg tcggcctgcg tggtgaacac
    13801 caccgacgtg ccgccgccgg tcgagacaac cgagccggtg accgaggcga acggctcggc
    13861 cgagccgtcc agtcgcagcc ccggtgcctg ctgcgcgttg acccacggac ccggatcgct
    13921 gaaccggccc atcggcgcgg ccggcgcgac ggtgtcgcgc aggacgttga cgccgctggt
    13981 cccgccgttg gcggacggat ccctcaccag caccgggaag atcccgggcg cgatcgcgga
    14041 ggtgtcgaac gggccgcagg agtagttcgg cgtgcccggt gagaacgacg ccgtcgcgca
    14101 cgccacgtac ggggcggcgc cgatcttgat ctcgagcggc gcgccggcct gcgcaacgcc
    14161 cgaggccgac agcgcgtctc tgaccggata cgggtcgatg tcgatgctcg gacggatcgg
    14221 gccggtgctc gtcgaggacg tgaccgtgac cgtggccgag gccgacgcgg gtccgacgtt
    14281 gccggccgcg tcgcgggcgc gcgccacgaa ggtgcgcgag cccggattgg acacgatcgt
    14341 gccgtgccac gcgcaggttc cgctcgcgca gcgggcccgc gcggtcgtcg cggcgacccc
    14401 gttgtcgtag agcaggacgt ccatgtgctc gaatccggcc tgcggcgaga cggttccggt
    14461 caccgtccac cgctggttgg cgttctgcag gccggccgtg ggccagtcga ccacgggcgc
    14521 ggccgggtag accacgtcct tgtaggcgta cgcgtagcgc ggggccgaga cgttgcccgc
    14581 ggggtcggtg agcgtgacgg tggcctcgat cgacaaggtg ctcacgaccg tggagggaac
    14641 gggagagtcg tcgagcccgg tgacgttgaa cggtccgacc gcgaacaggc cgcccgagcc
    14701 gactacgccg caagcgggct ggccggacac gccgcaggcg acggccgggg tggagccgtt
    14761 gtcgtcgtcg atgagcacga agacgctcgc acccggctcg ccggttccgg tcacgtagac
    14821 cttggtgcgg ttcgagtcgt tgatcggatg ggtcatggcg ggatccggcg ggaccggcgc
    14881 gatcccgtcg gagtagaagg tcgcggtgtg ggagacgctg tccccgccga tggtcacgcc
    14941 gaaggcatag atcgtgaatt ggccgctgcc gagcgtctct gtggggccga tcacgatggt
    15001 gcggcagtcg ggatacgaga ccatcacgct gatcagcgtg ccttgcgggc tgcgaagctc
    15061 cagcgacgag cgcggcaggt ccacgcactg gttgaagcgc acccggatct cggccggcgg
    15121 gaacaccgtc gagccgttgg ccggcgtgag ctgaaccacc acgagcggag ccgccgtcgc
    15181 cggcatcgga gccagcaatg cggccgcaac cgcgagcgcc acgaagcccg cgcgaaccct
    15241 ggaacgcaag gtcataatcc ctcccgatga atcacgttcg attcgccccg ggccgacccc
    15301 tttgaccctg ccggattacc gccatttgtt ctgctttcga tatcgggacg gagcggaagg
    15361 gacgaacgcc gcgccacggg ccccgtccgg caacacggtg gtttgctccc acggcgtcgt
    15421 tccggaagaa tccgaacgcg ccgcggcgtc gccgcgggac gcgcacgacc cacgagacga
    15481 acaggagatc gagccgatgg cgaacgtcga agcagagatg gtggccaacg tctggaaggt
    15541 gctcgtgcag cccggggcga ccgtcgccga gggcgacacg ctcgtgatcc tggaatccat
    15601 gaagatggag atcccggtcg aggccccgca cgacgggacc gtcacggcgg tcaacgtcca
    15661 tgagggcggc gtcgtgcaag aaggcgacgt catcgcggtc atcgagtgag cgatctcgtc
    15721 gagatcgttc ccggccgggt cacgctgatc acgctccggc ggcccggccg acgcaacgcg
    15781 ctcaacacgg cgatgctcga cgcgctgcgc gaggcgctcg ccgtcgcgga caccgacgag
    15841 caggcacgct gcatcgtgct gcagggcgag ggcgatcact tttgcgccgg cgccgacttc
    15901 ggcgatgtca ccgccggcgc cgagggcggc gcgcgctacg gtgccggctt cgaggcgctt
    15961 ctgcgctcca tcgaggagca ccggcttccc gtcgtcgccg gcgtgcaggg cgccgcgctc
    16021 ggcgcgggct gccagctgct cgcggcgtgt gacctggcgg tggcggccga ggatgcccgc
    16081 atcggcatcc cgagcgcccg gctcgggctg ctgctggacc tcgagaagat ccagcggatg
    16141 gccgccgtcg tcggcatccc gaccgtgcgc gagatgctgc tcgccggccg cgaatggacc
    16201 ggcgtcgaag cggccgagcg cgggctggtg accgccgccg tgccgcgcag cgcgctcgca
    16261 gccgccgtgg ccgggctcgc cgaacgggtc gcgtcgtgcg cgccgctgtc ggtgcaaggc
    16321 agcaaggccg ggcttcgcgc gatcgtcgat cacggtccgc tcgaccggat gcgagacgga
    16381 gcggccttcg agcgccatga tgagcgcgcg atcgcagcgg cgcagagcga ggacctgcgt
    16441 gaagggctcg ccgcgcttcg cgagcggcgg ccgcccgagt tccgcggccg ctaacgaact
    16501 gcgtcgcgca ccgccgacgc gaccgcgtcg gccgcgagca tccccagcag atcgacttcg
    16561 cgccgggtcg cgggccgctc gccgggcgcg gcgaccgcga acaccacgtc gccgtcgtag
    16621 tgcgtgtgcg ccggccgcac cgcgcgcgtg atcccgtcgc tgccgcgcgc cgccacgaag
    16681 gctgcttccc gcttgccgat cgtcgcggtc gtcgtcacca ccgcgagcac ggtgttctcc
    16741 gaccacgacg cggcctcgac gtcgcgcagc gacggccggg ccgctcgcgt gccggcaaga
    16801 acggtgccgt cgtcgccgac gatgtcgccg aacgcgttga ccaccgcgag cgcgcagacg
    16861 cgaacgtcgt cctgctccgc gctcgccacg cccagcccgc cgggtccccg atgatccagc
    16921 cctgcccact tgccgatcga cgcgcccgtg cccgcgccga cgcggccgcg cgcgacctcg
    16981 ccctcgattg cggcctcaca cgccgcatag ccttcctcgg ggccgggtct gcgcatgccc
    17041 ggcgcggccg cgaagtcgaa caccacggcc gccgggacga tcgggatcac aaccgggccg
    17101 gcgcgaaagc cagcgccgcg ctcggcgcac cagcgcatcg cgccgtcggc cgcagccagc
    17161 ccgaacgcgc tgccgccgga cagcacgatc gcatgcacct cgcttcgcgg catgtgcggc
    17221 gcgagcgcgg ccagctcgcg cgagcccggc gccatgccgc gcacgtcgca ggacgacacg
    17281 ttgccgggcg gcggcaggac caccgtgcag ccggtcggca ccgactcgtc ggtccagtgt
    17341 ccgacgcgaa atccgtcact catcgatgcc atgtccgaac gctaccatcc acgctctccg
    17401 ggacggctat gctggcggcg ccatgtatcc gctccgtccg ctcgcacgcc ggttcacgga
    17461 cctggacgag gccgacgtgc tgcatctgca acggctcgtc gcggactggc agatgcttgc
    17521 cgacctcaat ttcgccgacc tgctgctgtt cgtgccgctt cgcgacgagg acggctacat
    17581 cgtgctcgcg caagtgcggc ccttcccggc gcagacgctc tatcaagagg acatggttgg
    17641 cacggtcgtg ccgcgccgcc cgcgcgtggc gcgcgcgatc ggcgaagggc ggatcgtgcg
    17701 cgaaggcgac cccgagtggc gcgagggtgt gccgattcgc gaagaggtga tccccgtccg
    17761 ccacggcgac cgcgtcatcg gcgcgatctc gagcgagcag aacctttcga ccgcacgcac
    17821 gccgtccaag ctcgagcttt cctatcttca ggccgcgggc gatctggcgc agatgatcgc
    17881 cgacggcgag ttccccttcc cgggcgagca gccggagaac gagattccgc tgcgcgtcgg
    17941 cgacggcttc atgcgcatcg acgaagcggg cgtggtcacc tacgtcagcc cgaacgcgat
    18001 cagcgcctac cgcaggctgg gcgtgaccgc gaacctcgtg gacgagccgc tcggcggatc
    18061 cgagatcgag gaggagagcg tgctggcccg gctcaagcag gggcggccgg tcaccagcga
    18121 ggttgaggcg aacggtgcgt tcgtgctgcg ccgcttcatc ccgatcgtgc acgagggccg
    18181 cgtcgagggc gcgctcgggt tcatccggga caccaccgag cttcgccggc gtgaccgcat
    18241 gctggtcctg aaggacgcca cgatccgcga gatccatcac cgcgtgaaga acaatctgca
    18301 aaccgtcgcg tcgctgctgc gcatgcagac gcgccgtgtc ggcactgccg aaggaaaaca
    18361 ggcgctcgag gaggcgatcc ggcgcatctc ctcgatcgcg ctcgtgcacg agacgctgtc
    18421 gcaggactcc agcgagatgg tcgagttcga tcaggtcgcg cagcgcgtgg tggacatggt
    18481 cggcgatacg ctcaccggcc ccgaccggcc ggtgcgtttc agcctgtcgg gatcggcggg
    18541 cgagatcgac gccgacgtcg ccacgccgct cgcgctcgtg ctcaacgagc tgtgccagaa
    18601 cgccgtcgat cacgctttcc cccacggcgg cggcaaggtc gaggtcacga tcgagcggcg
    18661 cggcctgaac tttcgcctga ccgtggccga tgacggcacc ggccttcccg acggcttctc
    18721 gctcgagggc ggctccaatc ttggcctgca gatcgtgcgg acgctcgtcg agagcgagct
    18781 cggcggcgag atcacgctct cctccgacgg tggaacgcgc gcccgcgtcg agatcccgct
    18841 ggcgcagccg tcgtgaccct gctgctgctg cactccgccg gcgcgaccac tgcgctctgg
    18901 gaccgcgtgc tcgagcatct cgacggcatc ccggcgttcg cgtggccgct gccgggacgc
    18961 gcgggcgcgg cctggcccga caacgagcga gagcgcgacg tcgccgcgta cgcgcgcgcc
    19021 atcatggcgc gcatggacgg cgagggcatc gagcgcgcca cgatcgccgg gcattcgctc
    19081 ggcggggccg tggcgctgca gatggcgctt gcccattccg atcgcgtcgc cgggctcggc
    19141 ctgtcgtgca cgggcgccag gctgcgcgtg ttgcctcacg tcctcgaagg cttcgccgac
    19201 ggcgtgcgcg acgtgatcga cttctggatc aacgtgcagt gcgggcccgc caccggggac
    19261 cgcgagcgca tcgtgatgcg ccggatgctg gagtcggccg gcatggagca gacctggtgc
    19321 gatcttcgcg catgcgacgg cttcgacgtc atggaccggc tgcccgagat cggcgtgcgc
    19381 acccaggtct tcgggggcgg tcatgacgtc gtcacgccgc cgaagtacgc gcgctatctc
    19441 gcggagcgga tcagcggcgc gcgcctgacg atcttccccg gcgccgggca cacggctccg
    19501 ctggagcatc ccgaggagat ggccggcgat ctggcggtgc tgtgggcggc gacggcgccg
    19561 cccgtggacg ctagcggccg ccgacgaccg tcgtcggctc tccgcccccc ggcgactgga
    19621 aggtcggatc ggtcggggtg ctgatcacgc gcgtgacggc gagccagatg atgaacagca
    19681 gcagcccggc cgtgatgatg cggccgatcc agctcttgcg tcgctcggcc ggctcggatg
    19741 cgaatccgac gggaccgttg tattcctcgc gcatggtcag ggaatggtag cgcgtgtggg
    19801 gtgagcaggc agggaccgtc gggccgggat cagacggcga cgcgctggcg cgtggcgcgg
    19861 ctgcggcgga gcgtccggcg ctcctcttcg gtcaggccgc cccagacgcc cgcgtcctgg
    19921 gcggtctcga tcgcccattc caggcagggc tcgcggacgg cgcacatcgc gcagatggcc
    19981 ttggcggcct cgatctgggc gagcgccggg ccggtggtgc cgatggggaa gaagatctcg
    20041 gggtcgacgt ctcggcagga ggcctggtga cgccagtcca tcgagatgct ctccaaggca
    20101 acttccccct cttctcgttt tgggcaccgg gctgccacgg tgatgacgtt gaaggcccgg
    20161 aagcggcgcg agtatatgcc tcggccgaaa gggctggcaa taccttcccg gggggctttc
    20221 cgcgtcccga gggcggtttt tctgcacgaa accgagcaag cggcgttgcg cttgtggcac
    20281 aagcctgatc gcgtgacgac ttcgcttatc aaaacataac aacagcaaca cccgaggaag
    20341 ggggagcccg tgaaggtcat cggccaccga ggagcggccg gtcgcgcgcc cgagaacacc
    20401 gttgccgggg tggccctcgc cttcgccgac ggggccgatg cggtcgaggt cgacgtgcgc
    20461 ctggccgccg acggcacccc ggtgatcatc cacgacgaga cgctcgaccg gaccgcgggc
    20521 atcgcggccc gcgtagccgc cctcacctcg gccgagctgg ccgggctcat cggcggggac
    20581 cgccggatcc cgacgctcgc cgaggtctgg gacgcggcct cggggcgggt cgtgctcgag
    20641 ctaaagggcg cctgggggag cggcgaggcg gtggaggccg cgcgggtcgt ggcggacttc
    20701 ctggcgagcc gtgacgtctc ggcggcggtc gtgtcctgct tcgacccggc ggcgcttgcg
    20761 gcgtttctcg atgccggagg aaccgcgcga acgggagtga tcacgctgga ggcgctggat
    20821 cccgtgtcca acatccgggc cgccgccgag ggcgggcatc acgtctgcta tatccccggc
    20881 accctcgtca cggccgagat cgtgcggctg gcgcacgccg ccggcaagga gatcgtggct
    20941 tggaccgtca acgaccccga cgtgatccgc gcgctcgcgg ccgccggagc cgacggcgtg
    21001 atcaccgatg acccgggcgc cgcgcgcgcc gccctcgccg cgcgctaacc gatcacgcgc
    21061 agcgcgccgc gctcgaccga gaagcgaaac tcggtgtcgc tgcccgccag gtcgccgtcg
    21121 acctggaagc gtgccggcgc gtcggcgcgc aaaacgaacg agtcggcgtc gtgcagcgta
    21181 gtgaccgcgc ggaagcgaac gtgacgggcc ggcccgaacg cgctgaagat cacgcgcaga
    21241 accgtggtga tgcgcatcgt gcgcagcgcc gtcaggtcca ggccgagatc cagccgcgcc
    21301 tcgggcacga cgcggaacgg gcgtgagccg aggaacgtgt acgggttcga gttgcccacg
    21361 atcgccgcgc gaatcccgtc ggtgatcttg ccgtcgggtc cggtcagctc gagcgccgga
    21421 cgcggacgcg aggaagggaa gaaatattcg cgcaggccgg aggagatgaa gaacgtctcg
    21481 ccgaagcgcc gcttggcgcg cgggttcgct tccacgcgcc gcacgacctc ggcgtcgaag
    21541 cccacgcccg cgcagaacgc gaacgcgcgc ccgttcacgc ggccgaggtt gaccgttcgc
    21601 ggcgctaggc cctcgcgcac gcgcgacagc agcatggccg tcgcctcgac cgaatccttc
    21661 gggtaaccga gcgtgcgcgc aagcacgttc gtgccgccgc ccgggatcgt cgcgagcggg
    21721 atgccggtcc cggcgagccc gttgacggct tcgttgatcg tgccgtcgcc gccgagcacg
    21781 cagaccagat cgaacgcatc agccgcggcc gcctcggcgg ccaagtcggt ggcgtggccg
    21841 cgcttggtcg tctcggcgag ctcgacgtgc agctcagacg acaaggcgcg cgcgatgacg
    21901 tcgcgcacgc ccggggtggt ggtggtggcg accggattcc agatcaggag cgcgcgcttc
    21961 acccgcggcc ccgaaccagc gcctcgacct cgccgggctg ggcatgtcgc ccgagcgact
    22021 tcttggagaa cacgaggtct ccgtccacgg tcacctcgaa cacgcctccg ccggacggca
    22081 cgatcgtgat gatttcgacg gattgctcca tctcaccgag cagggaggcc gccagaccga
    22141 cggcacggtc ggtgtagcct caaacggcgc aatactcgat gttgatcttc atcggatgcc
    22201 tccaggtccc gcgacacggt cgattccagg ctatcccaat cggcgggggc ggaaacgagg
    22261 ccttcgggcc gtggcgggac gggatcgtcg agggccggga cggggggatg gtcacgcggc
    22321 cgggggcgtc ctaatctttg cccatgcgac gaagcgatgt cctggacccg gcgcccccgc
    22381 gtcacggcac cgacggcgag gggcgcgggg gctggtcgtg gctggtcacc gccaaggacc
    22441 gcttcgaggc cgaggtgctg cgcggcatgc tcgaaaacgc cggcgtgccg gtaatcctgg
    22501 acggcttcga tcgcagcccg ttcgcctgga tgtatcccgc gggcaacatg aacgtgccgg
    22561 tccaggtgct cgttccgacc gggctgctcg acgtcgcgcg gctcgagctg atggaggccg
    22621 ggctggtcgg ggtggaccgg ctcgcacccg cttccgcgca ggccgcggcg gccgccggcc
    22681 cgccggcgag cttgctccgc gaccggacgc gccctttgtg gctggtcgtg gcggtgctga
    22741 ccgcgctggc ggtcgccttg ttcatcatcg ttgaggtcgc cggcttcgct ccctgcgcaa
    22801 ccaaggtgtt ctgcatcaaa tgaggcgagt gcgggcaacg gcccgccgtg ccggaaggga
    22861 gcgcggtcgc ggccgcgaac tctccggcgt gatcgtcccc cgttcggtgt cgctggcgct
    22921 cgcggtcgtg ctcacggccg gtgtgcccgc atcggcttcg cagcgtcagg tcacggggcg
    22981 ggtcgagatt ccgacattcg gatcgcccgc gctgccgcgg accctctacg gcgcaacgca
    23041 atcgaacggc ctcgtgggct gggtcctgcg ggtgacgccg gggcgttcgt tcacgctttc
    23101 gacccaagcg gcgcccatgg gcgtggaaga cttcgacatc gcgttctacg cctcgctcga
    23161 ctcgtccgcg cccctgg
//
LOCUS       DATLFO010000016         4221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_139108, whole genome shotgun sequence.
ACCESSION   DATLFO010000016 DATLFO010000000
VERSION     DATLFO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139108"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VM841_00595"
     CDS             complement(<1..1442)
                     /locus_tag="VM841_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HVL88709.1"
                     /translation="MSDVAAPVAARGLRRLARNTAVYAAADLIAKGGAYLLMPILTRG
                     LSEVEFGRYTLVISIVPVLTLIASLGLGGAVTRLWFDLDEAARKRFQLAAWLGVQGTA
                     LAVALLLTAASGLLFRTLFPGPPARIFLYGVWGSFFLAAATVPLAVLRAEERPKRFAV
                     LSGSQFLLPSAAMVAALVAGAGLDDVLKAYLTGTAGVFALAAAMMAPLSARPADRSML
                     KPALALGLPVIPHLVAHWALNVVDRVAIQRELGAAAVGLYAVGYQIAQGVSLIATAVN
                     NATVPSFYRAAGRGRRDALGRAWAPKLYGLGAISLGVALLAPELATIFAAPQYSRTAE
                     FIPWVALGYWLLAAYYFPVNALFYAKRVRMIPVATITAAVANYVLNTVMLPRYGLIAA
                     AWNTAISYGLLLAIVLLAAHDQPSARRVEREHRPLQQRSHGTVRQQRRQRVHTRAQRA
                     DGDRLAEPIRKRPHALRGEQHDREKQAVADR"
     gene            complement(1439..2182)
                     /locus_tag="VM841_00600"
     CDS             complement(1439..2182)
                     /locus_tag="VM841_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HVL88710.1"
                     /translation="MRIVAIVQARMGSTRYPGKTLQDLGGTTLMERVLARVSATARID
                     EVVVATTTEAGDDPVEAVARAAGYPVVRGSVDDVLDRYVVAARAHRADVVVRNTADEP
                     FLDPAIIHEAIATFEDARPDYVSNNLHHVYPEGLDTEVIAMEALERAHREATLRSDRE
                     HVTPYVWRQPEVFRLVAAGGEPVHPEWRLTVDYPEDLAFARAIYEAIGGSLFGLPEIV
                     ALLGSRPDLLAMCPSVPRREGYDASREAE"
     gene            complement(2179..3210)
                     /gene="pseG"
                     /locus_tag="VM841_00605"
     CDS             complement(2179..3210)
                     /gene="pseG"
                     /locus_tag="VM841_00605"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HVL88711.1"
                     /translation="MARILIRTDGGAGIGLGHLQRCLSLATALQRAGALCFFAIHGDE
                     AAAARALESGFASRLLSTPSDDIAAVATEWECDAVVVDSYETGGALLTSLRDAGLFVA
                     AIDDLAAYPLDAHLVVSFGPHAHEANYESVRSDTRFLLGPRYTILRPEYWDVPLMPPR
                     ERVRNILVTTGGADPTGVMPSWIEAVDGIDADFDVTAIVGPFFAEPGAVHAAAAAAAR
                     TVRVVDSPRVTRPMQAMADIAVTAAGQTIYELAALGVPTVAAEIAANQRPQLDAFAKL
                     GAMIDAGAASDAATAERIATIVERLLSGADERRVLSETARAAVDRRGAVSVAAEIAGG
                     IAAHTKGSR"
     gene            complement(3215..>4221)
                     /locus_tag="VM841_00610"
     CDS             complement(3215..>4221)
                     /locus_tag="VM841_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVL88712.1"
                     /translation="QEGRAVGAGREADRAGRGDVAGPDAGGVTATSAISFAASANCAA
                     FLGATPRFADVDPDTINIDPVSLLALGPVKAVVPVDFAGLPIETATVVAAAGEAVVIE
                     DAAHGLGARYADGSPVGSCPRGIAMTAFSFHPVKSITTGEGGAVTTNDPALADTMRRF
                     RHHGIERDRSKFVYDDEGPWYHEMHDLGFNYRITDFQCALGLTQLRKLDAFVEARNEV
                     AAMYRERLASIDEITLPAAATGNGRHAYHLFAIRVPANLRRAVFEALRADGLGVQVHY
                     LPIYRHPWYRETFGYRPGLCPGAEAYYAGAISIPVYPGMSKDDVERVARSLEAALRAG
                     "
BASE COUNT          631 a         1482 c         1544 g          564 t
ORIGIN      
        1 cgatcagcta cggcctgctt ctcgcgatcg tgctgctcgc cgcgcagcgc gtgtggccgc
       61 ttccgtatcg gttcggcaag acgatcgccg tcggcgcgct gggcgcgggt gtgtacgcgc
      121 tgtcgacgtt gctgccggac ggtaccgtgg gaacgctgct gaaggggccg gtgttcacgc
      181 tctacgcggc gggcggatgg ctgatcgtgc gcggcgagca gcacgatcgc gagaagcagg
      241 ccgtagctga tcgcggtgtt ccatgcggcg gcgatcaggc cgtagcgggg aagcatcacc
      301 gtgttgagca cgtagttggc gaccgcggcg gtgatcgtgg cgaccgggat catgcgaacg
      361 cgcttggcgt agaacagcgc gttgaccggg aagtaatagg cggcgagcag ccagtagccg
      421 agcgcgaccc acgggatgaa ctcggcggtc cgcgagtact gcggcgccgc gaagatcgtg
      481 gcgagctcgg gcgcgagaag cgcgacgccg agcgagatcg cgccgagccc gtagagcttg
      541 ggcgcccacg cgcgtccgag cgcgtcgcgc cggccgcgtc cggccgcgcg gtagaacgac
      601 ggcaccgtcg cgttgttgac cgcggtcgcg atcagcgaca cgccttgcgc gatctgatag
      661 ccgaccgcgt agaggccgac ggcggccgcg ccgagctcgc gctggatcgc gacgcgatcg
      721 acgacgttga gcgcccagtg cgcgaccaga tgcgggatga ccggcagccc gagcgcgagc
      781 gcgggcttga gcatcgaccg gtccgcgggc cgcgccgaca gcggcgccat catcgcggcc
      841 gcgagcgcga acacgcctgc ggtgccggtc aggtacgcct tcagcacgtc gtccaagccg
      901 gcgccggcca ccagggccgc gaccatcgcg gccgagggca gcaggaactg cgagcccgag
      961 agcaccgcga accgcttcgg ccgctcctcg gcgcgcagga cggcgagagg gacggtggcg
     1021 gcggccagaa agaacgaccc ccacacgccg tacaggaaga tccgcgccgg cgggccgggg
     1081 aacagcgtgc gaaacagcag gccggacgcg gcggtcagca ggagcgcgac ggcgagcgcg
     1141 gtcccttgca cacccagcca ggcggcgagc tggaaacgct tgcgcgcggc ctcgtccaga
     1201 tcgaaccaca gccgtgtgac tgcgccgccc aggccgagcg aggcgatcag cgtgagcacg
     1261 ggcacgatcg agatcaccag cgtgtagcgg ccgaactcga cctcgctcag gccgcgggtc
     1321 aggatcggca tcagcaggta ggcgccgccc ttggcgatca ggtcggcggc ggcgtagacg
     1381 gcggtgttgc gggccaggcg cctcagcccg cgcgcggcga cgggcgccgc gacgtcgctc
     1441 attcggcctc ccgcgaggcg tcgtagcctt cgcggcgcgg aaccgacggg cacatcgcca
     1501 gcagatccgg ccgcgacccc agcagcgcca cgatctcggg caggccgaac agcgagcccc
     1561 cgatcgcctc gtagatcgcg cgcgcaaacg cgagatcctc gggatagtcc acggtaaggc
     1621 gccactcggg atgcacgggc tcgccgccgg cggcgacgag ccggaacacc tcgggctgcc
     1681 gccacacgta gggcgtgacg tgctcgcggt cgcttcgcag ggtcgcctcg cgatgcgcac
     1741 gctcgagggc ctccatcgcg atcacctcgg tgtcgagccc ttccgggtag acgtgatgca
     1801 ggttgttcga cacgtagtcg ggccgcgcgt cttcgaaggt cgcgatggct tcgtggatga
     1861 tggcgggatc caggaacggc tcgtcggcgg tgttgcgtac cacgacgtcc gcgcgatgcg
     1921 cgcgcgcggc gaccacgtac cggtcgagca cgtcgtccac gctgccgcgc acgaccggat
     1981 agcccgccgc gcgcgcgacg gcttcgaccg gatcgtcgcc cgcctcggtc gtggttgcga
     2041 ccacgacctc gtcgatgcgt gccgtagccg agacgcgcgc aagaacgcgc tccatcagcg
     2101 tcgtgccgcc gaggtcctgc agggtcttgc ccggataccg tgtcgagccc atccgggcct
     2161 gcacgatcgc cacgatcctc atcggcttcc tttcgtgtgc gccgcgatgc cgccggcaat
     2221 ctcggccgcg acggagacgg ccccgcgacg gtcgaccgcg gcgcgggccg tctccgacag
     2281 cacgcgccgc tcgtccgcgc ccgaaagcag ccgctccacg atggttgcga tccgctccgc
     2341 cgtcgccgcg tcgctcgccg cgcccgcgtc gatcatcgcg ccgagcttgg cgaacgcgtc
     2401 gagctgcgga cgctggttgg cggcgatctc ggccgcgacc gtcggcacgc cgagcgccgc
     2461 gagctcgtag atggtctggc cggccgcggt gaccgcgatg tcggccatcg cctgcatcgg
     2521 ccgtgtcacg cgcggcgagt ccaccacgcg aaccgttcgt gccgcggccg cggccgcggc
     2581 gtgcaccgcg ccgggctccg cgaagaacgg tcccacgatc gcggtgacgt cgaagtccgc
     2641 gtcgatgccg tccaccgctt cgatccagga gggcatcacg cccgttgggt cggcgccgcc
     2701 ggtggtgacc aggatgttgc gcacccgctc gcgcggcggc atcaacggca cgtcccagta
     2761 ttcgggccgc aggatcgtgt agcgggggcc gagcaggaag cgggtgtcgc tgcgcaccga
     2821 ctcgtagttc gcctcgtgcg cgtgcgggcc gaagctcacg accagatgcg catccagcgg
     2881 gtaggccgcg aggtcgtcga tcgcggccac gaacaagccc gcatcgcgca gcgaggtcag
     2941 cagcgcgccc ccggtctcgt atgagtccac cacgacggcg tcgcattccc actcggtcgc
     3001 gaccgcggcg atgtcgtccg acggcgtgga aagcagccgc gaggcgaagc cgctctcgag
     3061 cgcccgcgcc gccgccgcct cgtcgccgtg gatcgcgaag aagcacaagg cgcccgcgcg
     3121 ctgcagcgcg gtcgcgagcg acaggcaccg ctgcagatgg ccgagcccga tgccggcgcc
     3181 gccgtcggtc cggatcagga tgcgcgccac ggccctatcc ggcgcgcagc gccgcttcca
     3241 gcgagcgggc gacgcgctcg acgtcgtctt tgctcatgcc ggggtagacg gggatcgaga
     3301 tggcgcccgc gtagtaggcc tcggcgcccg ggcacaggcc gggccggtat ccgaacgtct
     3361 cccggtacca gggatggcgg tagatcggca ggtaatgcac ttgcacgccg agcccgtcgg
     3421 cgcgcagcgc ctcgaagacg gcgcggcgca ggttcgcggg aacgcggatc gcgaacaggt
     3481 ggtacgcgtg gcgtccgttg ccggtcgcgg ccgccggcaa cgtgatctca tcgatggacg
     3541 ccagccgctc gcgatacatt gccgcgacct cgttgcgcgc ctccacgaac gcgtcgagct
     3601 tgcgaagctg ggtgagcccc agcgcgcatt ggaagtcggt gatccggtag ttgaagccca
     3661 gatcgtgcat ctcgtgatac cacggccctt cgtcgtcgta gacgaacttg gaccggtctc
     3721 gctcgatgcc gtggtgacgg aagcggcgca tcgtgtcggc gagcgccgga tcgttggtcg
     3781 tgaccgcgcc gccttcgccc gtggttatcg acttgaccgg gtggaacgag aacgcggtca
     3841 tcgcgatccc ccgcgggcag gatccgaccg gggagccgtc ggcgtagcgc gctcccaggc
     3901 cgtgcgcggc gtcctcgatc acgaccgcct cgcccgccgc ggccacgacc gtggccgtct
     3961 cgatcggcaa gccggcgaag tccacgggaa cgaccgcctt gaccggcccg agcgcgagca
     4021 gcgacacggg atcgatgttg atcgtgtcgg gatccacgtc cgcgaagcga ggcgtggcgc
     4081 ccaggaaggc cgcgcagttg gcgctggccg cgaagctgat cgcgctggtc gcggtgacgc
     4141 cgccggcgtc gggcccggcg acgtcaccgc gaccagcgcg atcagcttcg cggccagcgc
     4201 caactgcgcg gccttcctgg g
//
LOCUS       DATLFO010000017         3582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_20491, whole genome shotgun sequence.
ACCESSION   DATLFO010000017 DATLFO010000000
VERSION     DATLFO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20491"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VM841_00615"
     CDS             complement(<1..995)
                     /locus_tag="VM841_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88713.1"
                     /translation="MLIGTTIALVPASALAASGRVTLEVPNASGTQFGFDVETAGATP
                     ATAASTDVRTHTSTTVGYRQLVALNGGGVAKLATTDFAAVGCVEMRGAALNGTVFTGS
                     DGSTDPVPGTIYVVRSNLGNYAKMQLVSIKTTAPKGLVLDFETSDCPLDSTPPVITPS
                     VTGAEGFAGWYVGPVTISWTVTDEQSGISSTEGCDTAELSDDSRETTITCTATSAGGT
                     ASQSVTVSIDRTAPVVAYDGNRGTYELDEHVVVDCVASDDTSGIASDTCASVDAPAHE
                     LGPGTHTASASATDVAGNEGEGSAEFEVIVTPGGVARLIVKLITDTGLASTLRYKA"
     gene            complement(1161..3275)
                     /locus_tag="VM841_00620"
     CDS             complement(1161..3275)
                     /locus_tag="VM841_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVL88714.1"
                     /translation="MDTIARISLAVSIVMLASGLAMRRISFLTRLLMAGKPDPERFKG
                     VGSKLKAELVKVLGQKKLFQRLLPGTAHAFTFWGFLVVQITLVESIGEVFDREFAIPG
                     PGRWEALGFIQDIFMLAIMIALVTFAGIRIAQSPKREGRKSRFFGSHIWQAYVILFLI
                     FAVVSTVLVVRGARAALGTLPYPDGAFLSSWIGDRLSIYTTESLHLIEDWWLLGHLAV
                     VFGFLVLVVNSKHLHIFTSPLNVMFGRQPIALGPLKPLHIDMEKMTEETSLGVGKVED
                     FTWKQMLDGYTCTECGRCQAACPAWNTGKELNPKLIITNLRDHLFEKSDYLLGKATEE
                     EAADVLAKSLVPDVISSEALWACVTCGACVQECPVDIEHVDAIVDMRRYQVMMESQFP
                     SEAGVMLRNLENSGNPWGAAASARMDWAKGIEDFVPVVNGRIPPEAEYLYWVGCAGAF
                     DDRAKKAVNAFARLMVEAGVGFAVLGPQESCTGDPARRIGNEYLFQEMAKANIATLKE
                     KGVRKIVASCPHCFNTMAREYPQFGGTFEVLHHSQLLERLVADGKIVPTKELEARITY
                     HDPCYLARHNDVIDEPRHLLDNVPGLAKQEMPRCRKGTFCCGAGGARMWMEETEGKRI
                     NHERIDEALETNPDMVSTGCPFCMIMLDDAVKDRAQQGKASEDLKVYDVAQILAQSVA
                     LNKTGGAAPRPPAAAVDAPAPE"
BASE COUNT          577 a         1152 c         1231 g          622 t
ORIGIN      
        1 gccttgtatc tgagcgtgga cgcgagaccg gtgtcggtga tcagcttcac gatgagccgg
       61 gcgacgccgc ccggcgtgac gatgacctcg aattcggccg atccttcgcc ctcgttgccg
      121 gcgacgtcgg tcgcgctcgc cgatgcggtg tgcgtgcccg gtccgagctc gtgggctggc
      181 gcatccacgg acgcgcatgt gtccgacgcg atcccgctcg tgtcatcgga ggcaacgcag
      241 tcgaccacga cgtgctcgtc gagctcgtag gtgccgcggt tgccgtcgta ggcgacgacg
      301 ggggcggtcc ggtcgatcga gaccgtgacg gattgggagg cggtgcctcc ggcgctggtt
      361 gcggtgcacg tgattgtcgt ctcccgggaa tcgtccgaga gctcggccgt gtcgcatccc
      421 tcggtcgagg agatgccgga ttgctcgtcg gttaccgtcc acgagatcgt gaccggaccg
      481 acataccagc ccgcaaatcc ttccgcgccg gtcaccgacg gggtgatgac cggcggggtc
      541 gagtcgagcg ggcagtcgga ggtttcgaag tcgagcacaa gacctttggg tgcggtcgtc
      601 ttgatcgaga cgagctgcat cttggcgtag ttcccgaggt tggagcgtac gacgtagatc
      661 gttcccggaa ccgggtccgt cgagccgtca cttccggtga acaccgttcc gttgagggcc
      721 gctccgcgca tctcgacaca gccgaccgcg gcgaagtcgg tcgtggcaag cttggcaacg
      781 ccaccgccgt tgagtgcgac gagctggcgg tagccgacgg ttgtgctcgt gtgggttcgc
      841 acgtccgtgg aggcggcggt ggccggagtc gccccggcgg tctccacgtc gaagccgaac
      901 tgcgttcccg aggcgttcgg gacctccagg gtgacccggc cgctcgcggc cagcgcgcta
      961 gccggaacga gcgcgatcgt ggtgccgatg agcatggtcg cgaggcttcg tcgaagtgtc
     1021 tcggatccca tacgccggac ctcccgcatc gatgtgcttt tggtatcggg agtttcgcgc
     1081 gaaatctcga gagccgggct ggctaattcg cactagtcca tatggactag tgcgcacgca
     1141 tcggcacgaa tcggacgccg ctattccggc gccggagcgt cgaccgccgc cgcaggcggc
     1201 cggggagcgg ccccgccggt cttgttgagg gcgaccgact gcgccaggat ctgggccacg
     1261 tcgtagacct tcaggtcctc gctggccttc ccctgctgcg cgcggtcctt gaccgcgtcg
     1321 tcgagcatga tcatgcagaa ggggcatccg gtcgagacca tgtcgggatt ggtctcgagt
     1381 gcctcgtcga tgcgctcgtg gttgatgcgc ttgccctcgg tctcctccat ccacatgcgc
     1441 gcgccgccgg cgccgcagca gaacgtaccc ttgcggcagc gcggcatctc ctgcttcgcc
     1501 agccccggca cgttgtcgag cagatggcgg ggctcgtcga tgacgtcgtt gtggcgggcc
     1561 aggtagcacg ggtcgtggta ggtgatcctg gcctcgagct ctttggtcgg cacgatcttg
     1621 ccgtcggcga ccagacgctc cagcagctgc gagtggtgga gcacctcgaa ggttccgccg
     1681 aactgcgggt actcgcgcgc catggtgttg aagcagtgcg gacacgaagc gacgatcttg
     1741 cggacgccct tctccttcag cgtcgcgatg ttggccttgg ccatctcctg gaacaggtac
     1801 tcgttgccga tgcggcgggc cggatcaccc gtgcaggact cctgcgggcc cagcaccgcg
     1861 aagccgacgc ctgcctcgac catcagccgg gcgaaggcgt tgacggcctt tttcgcacgg
     1921 tcgtcgaacg cgccggcgca gccgacccag tacaggtact cggcctcggg cgggatgcgg
     1981 ccgttgacga cgggcacgaa gtcttcgatg cctttggccc agtccatgcg cgcgctggcg
     2041 gcggcgcccc acgggttgcc ggagttctcg aggttgcgca gcatcacgcc cgcctcggac
     2101 gggaactgcg attccatcat cacctgatag cggcgcatgt ccacgatcgc gtcgacgtgc
     2161 tcgatgtcca cggggcattc ctgcacgcac gcgccgcagg tcacgcatgc ccacaacgcc
     2221 tcggacgaga tcacgtcggg cacgagcgac ttggcgagca cgtccgcggc ctcctcctcg
     2281 gtcgccttgc cgagcaggta gtcgctcttt tcgaaaaggt ggtcgcgcag gttggtgatg
     2341 atgagcttcg gattgagctc cttgccggtg ttccatgccg ggcacgccgc ctggcatcgt
     2401 ccgcactcgg tgcaggtgta gccgtcaagc atctgtttcc aggtgaagtc ctcgaccttg
     2461 ccgaccccca gcgaggtctc ctcggtcatc ttctccatat cgatgtggag cggcttgagc
     2521 ggcccgagcg cgatcggctg gcggccgaac atcacgttca gcggcgaggt gaagatgtga
     2581 aggtgcttgg agttcacgac cagcaccagg aaaccgaaca ccacggcgag gtggccgagc
     2641 agccaccagt cctcgatcag gtgcagcgac tcggtcgtgt agatcgagag ccggtcgccg
     2701 atccacgacg acaggaacgc gccgtccgga tagggaagcg tgcccagcgc cgcccgcgcg
     2761 ccgcgcacaa ccaggaccgt ggacacgacc gcgaagatca ggaacaggat cacgtaggcc
     2821 tgccagatgt ggctgccgaa gaagcgcgat ttgcggccct cgcgcttggg cgactgtgcg
     2881 atacggattc cggcgaacgt caccagcgcg atcatgatcg cgagcatgaa gatgtcttgg
     2941 atgaagccca gcgcctccca gcggccgggg cccgggatcg cgaactcgcg gtcgaagacc
     3001 tcgccgatcg actcgacgag cgtgatctgc accacgagga agccccagaa cgtgaacgca
     3061 tgggccgtgc ccggcaacag ccgctggaac agcttcttct gccccagcac cttgaccaac
     3121 tcggccttca gcttcgagcc gacgcctttg aatcgttccg ggtcgggctt gccggccatc
     3181 agcagccggg tgaggaagct gatgcggcgc atcgcgaggc cgctcgcgag catcacgatc
     3241 gagacggcga gcgagattcg agcgatggtg tccattagtg ggccaaaccc cctcggccga
     3301 aagcccgaat gcgggcgcgg gcacgaacgc cgcggattat atggcgggca ggggggccct
     3361 caaaaccgga aggaacgacg cggccgggcg gctcgtcata caatcggttc tctgcttcga
     3421 ctcgatcgga aaggatttcc acatgcgacg gatgaaggga atcgcggcag cgctcgtggt
     3481 gctggcgctg acggcctccg cgtgcggcgg cagcggcggc ggcggctccg actccggcgc
     3541 gggcaatccg gccgacgcgg tgctggtcgc ggccaagaag ac
//
LOCUS       DATLFO010000018        14007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_109555, whole genome shotgun sequence.
ACCESSION   DATLFO010000018 DATLFO010000000
VERSION     DATLFO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109555"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="VM841_00625"
     CDS             complement(<1..1598)
                     /locus_tag="VM841_00625"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88715.1"
                     /translation="MKGRFASVVPLQSSLVHRDRLFTYSIPESLDVHVGSLVRIPLGR
                     KRADGVVVALMDRPDVERTVPLIAALGRGLEEATVDLARWTAARYCASLGEALTAALP
                     ERVVSEEETSLPPLPHAEAGADWLKEWRGGPALLRALARSGRASFSMQPPPAEDRGPM
                     IASLSAAALSAGRGVLVLLPEVRFRSGVETALKAMFGDALAWLGSNHSNRVRYRGWGA
                     LRRGETRIACGGRAAVFAPVPDLGLIVVDDESHVSYKERRQPRFHARTVAAERARRSS
                     ATLVAVGVPPSIEVAAAVAARQMTVVAPAATQRRDHPPVQVIDLSESRERLVPAGRTI
                     AAAKQALSRGERVMIMLHRGGDEPARVFERAVRTTGAKAPALLDARSGAAVLRDALRK
                     ADLIVSSPVIAKDIPLQHAGLVAICHADAALAQTDFRTAEETFATWWRAARFAARVVI
                     ETADPSHPAVRALVRYDPAVLAADEIARRSELGYPPFGGLARIAAPAERASEIAESLE
                     REGATVLGPVERDGGAIIAVKAATR"
     gene            complement(1614..2801)
                     /gene="metK"
                     /locus_tag="VM841_00630"
     CDS             complement(1614..2801)
                     /gene="metK"
                     /locus_tag="VM841_00630"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HVL88716.1"
                     /translation="MARRWVYTSESVTEGHPDKMADAISDAILDAILKDDPMGRVACE
                     TFVTTGLCIVGGEISTTTYVDVPRIVRETIKDIGYTRAKYGFDGETCGVMNTIQDQSP
                     NIAVGVDNAYEARRGQADELDSQGAGDQGMMVGYACTDTPELMPAPIQIAHRMARRLA
                     AVRKSGDISYLRPDGKTQVTIEYEGNTPKRVVNVVVSSQHGPEIDIPTLLEPDIREQV
                     VEPILHETGLDYEDFQLLVNPTGIFEIGGPKADTGLTGRKIIVDTYGGMAPHGGGAFS
                     GKDPTKVDRSAAYAARWVAKNVVAAGFAERLQLEVAYAIGVARPVSVSVETFGTETVA
                     IEKIEKAINEVFDLRPAAILRDLDLRRPIYRQTSAYGHFGREEKDFTWERTDRVDDLK
                     SAI"
     gene            complement(2848..4065)
                     /gene="coaBC"
                     /locus_tag="VM841_00635"
     CDS             complement(2848..4065)
                     /gene="coaBC"
                     /locus_tag="VM841_00635"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009441243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HVL88717.1"
                     /translation="MSQVLTDVGALRGARILLGVTGGIAAYKAVEICRLLQKAGAEVR
                     VIMTKAATRFVGQATFAAITHHPAPADVFDDADQTLHVRLARWADLMIVAPATANTIA
                     KAANGIADDLVSSTLLMRAGRLLVAPAMHTEMWQHPATQANIATLRERGAAFVGPEEG
                     ELSIGDEGIGRLAEPPDVVAAAAALLASRGDMTGLRVLVTAGGTQEPIDPIRFIGNRS
                     SGKMGFAIANEAARRGAQVTLVTGPSALADPDRVTVVRVRTSQEMRNEVVARFDDCDV
                     VVKAAAVADFRPAKPSDHKLKKDEGAPDIALERTPDILAELGLTKTHQILVGFAAETQ
                     DAVAYARKKLAAKNLDMVVANVVTEPGAGFEHDTNRAMLIAADGSEEDLPLQSKAAMA
                     AVICDRIAVRLRS"
     gene            complement(4062..4304)
                     /gene="rpoZ"
                     /locus_tag="VM841_00640"
     CDS             complement(4062..4304)
                     /gene="rpoZ"
                     /locus_tag="VM841_00640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HVL88718.1"
                     /translation="MIEPRIDDLLGQVDSKYTLVIMAAKRARQINSYYAQLGEGIGEF
                     VPPLVHTEPGRKPLSIAMEEINAGKVAYERTSEGIK"
     gene            complement(4323..4931)
                     /gene="gmk"
                     /locus_tag="VM841_00645"
     CDS             complement(4323..4931)
                     /gene="gmk"
                     /locus_tag="VM841_00645"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVL88719.1"
                     /translation="MAAHRVRERKSGRRRLAIARLVVLSGPSGVGKSTVARLVAQRRP
                     EVFLSVSATTRRPRAGEQHGRDYLFLSDQEFADMESRGEFLETAEVFGANRYGTPRPP
                     VERALASGRPVLLEIDVQGARQVKKAMPEAVTVILLPPSWEVLQARLRGRGTEDAAAL
                     ERRLATAKSELALAETFDYRVVNDDLEDAVTQVDRILMGTPT"
     gene            complement(4883..5215)
                     /gene="mihF"
                     /locus_tag="VM841_00650"
     CDS             complement(4883..5215)
                     /gene="mihF"
                     /locus_tag="VM841_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HVL88720.1"
                     /translation="MPLPELTEEQRKQALEKAAEARKARAELKQQLKAGKLTLTQVLA
                     TEGDDTIGKMKVAAVLEALPGVGKVRAKKIMEKLDISSTRRIRGLGAKQRESLLTEFA
                     SGNPGEDD"
     gene            complement(5376..5960)
                     /gene="pyrE"
                     /locus_tag="VM841_00655"
     CDS             complement(5376..5960)
                     /gene="pyrE"
                     /locus_tag="VM841_00655"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVL88721.1"
                     /translation="MSDAEVREIFARYEAILTGHFKLSSGRHSDTYLQCARVLEHPRV
                     AHSLGAALANKLQARAERIDVVASPAMGGLLIGYAVAHALGCRFVFYERADGSFALRR
                     GQQLSPGEKVVVIEDVITTGGSAAEVVELVRATGADVVGVGSIVDRSAARPPFGLESL
                     LKVQAQSWEPDECPACGRGDAIDAPGSRHLKPGA"
     gene            complement(5957..6628)
                     /gene="pyrF"
                     /locus_tag="VM841_00660"
     CDS             complement(5957..6628)
                     /gene="pyrF"
                     /locus_tag="VM841_00660"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVL88722.1"
                     /translation="MTNPLIVALDVPTVEEASALAKQIGDAAGLYKIGLEMWNANGHD
                     AVRAVDGPVFLDLKLHDIPTTVQRAMRVLAPLAPAMVNVHALGGRAMLEAAAGAKPAG
                     TVLLAVTILTSLTDDDLFEIGLPPAGDAVPALAALAHEAGCDGIVCAPRDLARVREVV
                     PKPFVIVTPGVRPAGTDANDQVRVTTPAEAIAGGADYIVVGRPVTRADDPRAAAQAIV
                     GEATR"
     gene            complement(6625..7545)
                     /locus_tag="VM841_00665"
     CDS             complement(6625..7545)
                     /locus_tag="VM841_00665"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HVL88723.1"
                     /translation="MAVDLTVDLAGISLKNPVLSASGCFGSGKEISRFTDLHAMGAVV
                     CKTVTLRPRAGIAPPRGAETPSGMLNAIGLQNPGVDVFRDTDLVWLAQQGVPAIPSAG
                     GHSVDEYVAVVERLAGAPKVAVELNLSCPNLEERGYMFALDATRAAEVTRAVKAVADT
                     PIFVKLSPDVTDLVAIARAVADAGADGITLINTTLGMSIDIATGRPKLSTGTGGLSGP
                     AIRPIAVRCIFQVAHALPHVPILGAGGVASGRDAIEMIVAGATVVQVGTANFYDPRAT
                     QRIAAEIHTWCGQHGIDRIADMKGRVKLEG"
     gene            complement(7536..8303)
                     /locus_tag="VM841_00670"
     CDS             complement(7536..8303)
                     /locus_tag="VM841_00670"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HVL88724.1"
                     /translation="MTTAATIAQARVASVRTDGPYTILAFEAAEIAASAAPGQFVNVA
                     QDAFGCLLRRPFSILDVDGDRVEIGFDTIGEGTRRIAASAPGDRLDVVGPLGTAYTMP
                     APGGSALLVGGGYGAAPLFFLARLLRAQGTTSHMILGAATKGRLFAPDRGANAADTLT
                     ITTDDGSTGLRGRVTDAMDGIEADAIYACGPMPMLAAVAARANGRRCEVAVEEFMACG
                     VGVCWTCVVPTHDEGYRHHQRACTDGPVFDGSVIAWP"
     gene            complement(8519..9499)
                     /locus_tag="VM841_00675"
     CDS             complement(8519..9499)
                     /locus_tag="VM841_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88725.1"
                     /translation="MNDFGKRRRVRFAAAWLATAMALPVLGGAPAQAAITTPADGQTV
                     RGEVLIHENRGGVEDSVCAFAGARANSTITVTRLSDNATVLNISKGSAGPWSTTWDSR
                     PELANGLYRIQSVARDRGPWYLLCPTTVRVLSTITVRLENFMAFTDDLGGGTVSVNPY
                     LKVFRFVSDSYDSGNRHAPDMQIVPLPVAPQPPCDPSGDPAACAPAAPALPSLHPACA
                     DPTSPTFVPFLLQFGPQGCLSPNPVPPVTVPPALPEPPDPNNPPSPPENAQCRVGGCG
                     DVAIVIAFAGDDFALAGVFHAETRAFVAGAATLGEAPTATVLHHQPLPGA"
     gene            complement(9524..10423)
                     /locus_tag="VM841_00680"
     CDS             complement(9524..10423)
                     /locus_tag="VM841_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88726.1"
                     /translation="MTSTRRFLLTAVAAALLATTTAGNATRVQTVPERFRAMFEQKAA
                     EFGVDLETLLNAPATISVHGDGEVREYVRPFGEVLRNLYPDTAASSAMAQGPLGTPEY
                     AAGNITHLFATLWAGATSEAVANCQSVSVRRSQTVPDTYALASQAGGAAGVPVPPIVP
                     VTGAPLFLTPVPGLLSPVIPPASWILPAYAWLGPTMHITARYTLGLHQVGTLIGSTVS
                     TAQPAAAPYPVWLPMVSGASFAEDRSIDFVGQGLFFSASARLDAFGYTLCGGAGALAL
                     SNGAAIFDNHPVLGISAGLPDIP"
     gene            complement(10441..13278)
                     /locus_tag="VM841_00685"
     CDS             complement(10441..13278)
                     /locus_tag="VM841_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88727.1"
                     /translation="MRRVSLLLAAIMALSVQAPGLARSEILDLLSYKIHTVAEDAEGA
                     RVETSADAILLVPSLVDVDGDGIPDVAAQLQIDAARATLRVTKLPSARAELPLLIEAV
                     LRDPRQGSPYRVAFGYDTLDSVAPAVYSATIFLAGAGRAASFSLDVETAVAGPTLAVI
                     AEIFSEGSSGEREDPVRGRVSYAPVPTLSHFAGLVGSDFGVTQTSIDLTSNIDTTARV
                     LLQSLEGTRDTRAEATIDPLPAGTMSLVLNDSPLGTRTWDYTATARVQSIDLRISDSR
                     SDVLQSEFHLKIQDMALRATLVQDSPTHSVFRASDAIGLVEIGNATGGAIRWLDEQGH
                     YLYTDDSGGVDSLAVRVLGLSEFELETGDPSLLDARLASGPLHVLTKQGTQTIDAWVR
                     DLPSQFRIGFSSTQGTITYAGSAPIGELTVDATDPAGISGRATALHLLMRDIPTSMDL
                     TFGDGGSAIGLDARGNTLGLIEVQLTSGPDDRLPESVDGILLNDLSSHYALFARVTGL
                     RKVLVEQGPPPALQLNTVGGREFQIDLNTDKPGGRATTDAVIQILPTELSARFLSDTK
                     FTYTASQPVALITIDAFDPDGIAGRATELHARLESLPTAVDVGFDADGTVSVDAKGSV
                     LGLLEFQLSSIPNGDRIDAQFDGMLLRDIAGTGARYVVHARLTQIKRASAKAPNFSID
                     GLSTKIFKVDIERRPTATAKLEYLRVTLDHLVPNIVVSMVDVGGRSLMTYDAAQRTNG
                     LTMESNSGDRWNMSTSIMPLPTHLDFCSANSGACSNRPASDTRPSGQSPSASSSIHAS
                     EHVTVNIFDCQRPLSASCTQSGASKYFLIQNWQVRFFEQEFGGSDSSGYIWENTTDHG
                     PGCAATPQQSDSGACHHRILGGTIRQRDESAGTGLEQTFGNGNFWADQRQGGWCAYIF
                     SCNKKHYVNCSGGSLSVWLLNIKIGVSGYFC"
     gene            complement(13367..>14007)
                     /locus_tag="VM841_00690"
     CDS             complement(13367..>14007)
                     /locus_tag="VM841_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88728.1"
                     /translation="AVTANGFVYIADTGNDRIQVWDSFGIAKVAQFGTAGAAPGAFSS
                     PHGIAIVPDGPLAGSIAVADSGNDRIQIVSPTGAPQAVIGASGSDPGQVSDPKAVSVY
                     RGQVFVADTGNNRVQRFTIAGEPAGFWGGSRVQDGCMSAPLGIVATTYGVFVSDSGNQ
                     RLSHFSRTGSLLEQWAGAAAGIAVEPGGVMWAAATNGVRRYAPQAQTAPPLP"
BASE COUNT         2136 a         4680 c         4923 g         2268 t
ORIGIN      
        1 cgcgttgcgg ccttgaccgc gatgatcgcc ccgccgtccc gttccacggg cccgagcacg
       61 gtcgcgcctt cgcgttccag cgactcggcg atctcgctcg cgcgctcggc gggtgcggcg
      121 atgcgggcga ggccgccgaa cggcggatag ccgagctcgc tgcggcgggc gatctcgtcg
      181 gcggccagca ccgccgggtc gtagcgcacg agcgcgcgaa ccgccgggtg ggagggatcg
      241 gccgtctcga tcacgacgcg cgcggcgaag cgggcggcgc gccaccaggt ggcgaaggtc
      301 tcctcggcgg tgcggaagtc ggtctgggcg agggccgcgt cggcgtggca gatcgcgacg
      361 agcccggcgt gttgaagcgg gatgtccttg gcgatcacgg gagaggacac gatcaggtcc
      421 gcctttcgca aggcgtcacg aagcaccgcg gcccccgatc gcgcgtcgag caacgcggga
      481 gccttggcgc cggtggtgcg cacggctcgc tcgaagacgc gcgcgggctc gtcgccgccg
      541 cgatgcagca tgatcatcac ccgctcgccg cgggacaacg cctgcttggc cgccgcgatg
      601 gtgcgtcccg ccgggacgag ccgctcgcgg ctctcggaca ggtcgatcac ctggacgggt
      661 ggatggtccc ggcgctgcgt cgcggccggc gcgacgaccg tcatctgccg ggcggcgacc
      721 gcagccgcga cctcgatgct cggtggaacg ccgaccgcga ccagggtcgc gctcgagcgg
      781 cgggcgcgct cggccgcgac ggtgcgcgcg tggaagcgcg gctggcggcg ttccttgtag
      841 gacacgtgcg actcgtcgtc gacgacgatc aacccgagat cgggcacggg tgcgaacacc
      901 gcggcgcggc cgccgcacgc gatgcgcgtc tcgcctcgac ggagcgcgcc ccagcctcga
      961 tatcggacgc ggttcgagtg gttcgatccg agccatgcca gcgcgtcgcc gaacatggct
     1021 ttcagcgcgg tctcgacgcc ggaccgaaag cgaacctcgg gcagcaggac cagcacgccg
     1081 cgccctgcgg acagcgccgc cgcgctcagg gaggcgatca tcgggccgcg atcctcggcc
     1141 ggtggcggct gcatcgaaaa cgaggcacgc ccgctgcgtg cgagggcccg gagcagcgcc
     1201 gggcctccgc gccattcctt cagccagtcc gcgccggcct cggcatgtgg cagcggcggc
     1261 agcgaggtct cctcctcgga tacgacccgc tccggcagcg cggccgtgag cgcctcgccg
     1321 aggctcgcgc agtagcgtgc ggccgtccat cgcgcgagat cgacggtggc ttcttccagg
     1381 ccgcggccga gcgcggcgat cagcgggacg gtgcgctcga cgtcgggccg gtccatcagc
     1441 gcgacgacca cgccgtcggc gcgttttcgg cccagcggaa tgcgcaccag cgagccgacg
     1501 tgcacgtcca gcgactcggg aatcgagtac gtgaagagac ggtcccgatg cacgagactc
     1561 gactgcagcg gtacgaccga cgcgaaacgc cccttcatac ggggcaggct agatcagatc
     1621 gccgacttca ggtcgtccac gcgatcggtc cgctcccacg tgaaatcctt ctcttcgcgt
     1681 ccgaagtggc cgtacgccga cgtctgacga tagatcggcc ggcgaagatc cagatcgcgc
     1741 aggatcgcgg ccggacgaag atcgaagacc tcgttgatcg ccttttcgat cttctcgatc
     1801 gcgaccgtct cggtcccgaa cgtctcgacc gagacgctca ccggccgtgc gacgccgatc
     1861 gcgtaggcaa cctccagctg caaccgctcg gcgaagccgg ccgcaacgac gttcttggcg
     1921 acccagcgcg ccgcgtacgc ggccgagcgg tccaccttcg tcggatcctt gccgctgaac
     1981 gcgccgccgc cgtggggcgc catgccgccg taggtgtcga cgatgatctt gcggccggtc
     2041 aggccggtgt cggccttggg gccgccgatc tcgaagatgc cggtcgggtt gaccagcagt
     2101 tggaagtcct cgtagtcgag gccggtttcg tgaaggatcg gctcgacgac ctgctcccgg
     2161 atgtcgggct cgagcagcgt cgggatgtcg atctcggggc cgtgctggct ggagacgacc
     2221 acgttgacga cgcgcttggg cgtgttgccc tcgtactcga tcgtcacctg cgtcttgccg
     2281 tcgggacgca gatacgagat gtcgccgctc ttgcgcacgg cggccaggcg gcgcgccatg
     2341 cgatgtgcga tctggatcgg cgcgggcatc agctcgggcg tgtcggtgca ggcgtagccg
     2401 accatcattc cctgatcgcc cgcgccctgg gagtccagct cgtccgcctg gcccctgcgc
     2461 gcctcgtagg cgttatccac gccgacggcg atgttgggcg actgatcctg gatcgtgttc
     2521 atcacgccgc aggtctcgcc gtcgaagccg tacttggcgc gggtgtagcc gatgtccttg
     2581 atcgtctcgc gcacgatgcg cggcacgtcc acgtaggtgg tggtcgagat ctcgccgccg
     2641 acgatgcaca agccggtggt cacgaacgtc tcgcaggcca cgcggcccat cggatcgtcc
     2701 ttcaagatcg cgtccaagat cgcgtcgctg atcgcatccg ccatcttgtc cgggtgacct
     2761 tcggtcaccg attcggacgt atacacccac cgtctggcca tctcgcgtga tctccttcta
     2821 tggaagcctg agtcgagtat atgccgtcta gctccggagc cggaccgcga ttcgatcgca
     2881 gatcacggct gccatcgcgg ctttcgattg gagcggaagg tcttcctcgc tgccgtcggc
     2941 ggcgatgagc atcgcgcggt tggtgtcgtg ctcgaagccg gcgccgggtt cggtgacgac
     3001 gttggcgacg accatgtcca aattcttggc ggcgagcttc ttgcgggcgt aggcgacggc
     3061 gtcctgggtc tcggcggcga agccgaccag aatctgatgg gtcttcgtga ggcccagctc
     3121 ggcgaggatg tcgggcgtgc gctcgagtgc gatgtcgggc gcgccttcgt cctttttcag
     3181 cttgtgatcg ctgggcttgg ccgggcggaa gtcggcgacg gcggccgcct tcacgaccac
     3241 gtcgcagtcg tcaaagcgcg cgacgacctc gttgcgcatc tcctgcgacg tgcgtacccg
     3301 cacgacggtg acgcggtccg ggtcggcgag tgcgctgggc ccggtgacga gcgtgacctg
     3361 cgcgccgcga cgcgcggctt cgttcgcgat ggcgaacccc atcttgccgc tggagcggtt
     3421 gccgatgaac cggatcggat cgatcggctc ttgcgtgccg ccggcggtga cgagcacccg
     3481 caggccggtc atgtctccgc gcgaggcgag aagcgcggcc gcggcggcga caacgtcggg
     3541 cggctcggcg aggcggccga tgccttcgtc gccgatcgac agctcgccct cttccggccc
     3601 gacgaaggcg gcgccccgct cgcgcagcgt cgcgatgttt gcctgcgtcg ccgggtgttg
     3661 ccacatctcg gtgtgcatcg cgggcgcgac cagcagacgg ccggcgcgca tcagcagcgt
     3721 cgaggagacc agatcgtcgg cgatgccgtt cgcggccttg gcaattgtgt tcgcggtcgc
     3781 gggcgcgacg atcatcaagt cggcccagcg cgcgaggcgc acgtgcagcg tctggtcggc
     3841 gtcatcgaag acgtcggcgg gtgcgggatg gtgcgtgatg gcggcgaagg tcgcctggcc
     3901 gacgaagcga gtggccgcct tggtcatgat cacgcggacc tcggctccgg ccttttgcag
     3961 caagcggcag atctcgacgg ccttgtaggc cgcgatgccg ccggtgaccc ccaacagaat
     4021 gcgagcgccc cgaagggcgc ccacgtcggt taggacctgg ctcacttgat cccttcgctg
     4081 gtccgctcgt aggcgacctt gccggcgttg atctcctcca tcgcgatcga cagcggcttg
     4141 cggccgggct cggtgtggac cagcggcggg acgaactcgc cgatgccttc gccgagctgc
     4201 gcgtagtagg agttgatctg ccgcgcacgc ttggcggcca tgatcaccag cgtgtacttg
     4261 gagtccacct ggccgagcag gtcatcgatc cgaggttcga tcatgggtgg tgggttcctt
     4321 ccttacgtgg gagttcccat cagtatacgg tcaacctggg tgaccgcgtc ctccagatcg
     4381 tcgttgacga cccggtaatc gaaggtttct gccagggcga gttcgctctt ggcggtggcc
     4441 aggcggcgct ccagcgcggc ggcgtcctcg gttccccggc cgcggaggcg ggcctggagg
     4501 acctcccacg aaggtggcag aaggatcacc gtgacggcct cgggcatcgc ctttttgacc
     4561 tggcgggcgc cttggacatc gatctcgagc aggacgggcc gacctgaggc cagcgccctt
     4621 tcgaccggag gccggggcgt gccgtagcgg ttcgcgccga acacttctgc ggtttccagg
     4681 aactcgccgc gggactccat gtccgcgaac tcctgatcgc tcagaaacag gtagtcgcgg
     4741 ccgtgctgct cgcccgctct gggccttctg gtggtcgccg aaaccgagag aaacacctcg
     4801 ggacgacgct gagcgaccag ccgtgcgacg gtcgatttgc cgacgcccga cgggccgctc
     4861 aggacgacga gacgcgcgat ggctagtcgt cttcgcccgg atttccgctc gcgaactcgg
     4921 tgagcagcga ttcccgctgc ttggccccca gaccgcgaat ccggcgcgtg gatgagatat
     4981 cgagcttctc catgatcttc ttggcgcgta ccttgccaac ccctggcagc gcctcgagga
     5041 cggctgcgac cttcatcttc ccgatcgtat cgtcaccttc ggtggcgagg acctgggtca
     5101 gggtcagctt cccggccttg agctgctgct tcagctcggc acgtgctttc cgggcttcag
     5161 cagccttttc caaggcttgc ttccgctgct cttccgtgag ctcgggaagc ggcataattc
     5221 cccctccttg caaaccgacc tctgtgtcag gacgggcgcg agtctatcat cggggtgaga
     5281 ccccttcaaa cacgtgcccg acctgttttc gcaggtcaac ggccatattt cgcgatcatg
     5341 ccctgccgga cgccgggtga gatgcccggt ttgggctatg cgccgggctt gaggtgccgg
     5401 ctgccgggcg cgtcgatcgc gtcgccgcgg ccacacgcgg ggcactcatc gggctcccac
     5461 gactgtgcct ggaccttcag cagtgactcc aggccgaagg gaggccgcgc cgcgctccgg
     5521 tcgacgatcg agccgacccc gacgacgtcg gcgcccgtgg cgcgaaccag ctcgaccacc
     5581 tccgcggcgg atccgccggt ggtgatcacg tcctcgatga cgacgacctt ctcgcccggg
     5641 gacagctgct ggccgcggcg cagcgcgaag ctgccatcgg cccgctcgta gaacacgaac
     5701 cggcagccga gcgcgtgcgc gaccgcgtag ccgatcagca gcccgcccat cgccgggctc
     5761 gcgacgacgt cgatccgctc cgcccgggcc tggagcttgt tcgccagcgc cgcgccgagc
     5821 gagtgggcga ctcggggatg ctcgagtacg cgggcgcatt gcagataggt gtcgctgtgc
     5881 cggccgctcg agagcttgaa gtgcccggtc aggatcgcct cgtagcgggc gaagatctcg
     5941 cggacctctg cgtcgctcat cgcgtcgcct cccccacgat cgcctgcgcg gccgcgcggg
     6001 gatcgtcggc gcgggtgacg ggacggccga ccacgatgta gtcggcgccg ccggcgatcg
     6061 cctcggccgg cgtggtgacg cgcacttggt cgttggcgtc ggtccctgcg ggacgcacgc
     6121 ccggcgtgac gatcacgaac ggcttcggga cgacctcgcg gacccgcgcc agatcgcgcg
     6181 gcgcgcatac gatgccgtcg cagcccgctt cgtgcgcgag cgcggccagc gccggaaccg
     6241 cgtcgcctgc gggcggcagg ccgatctcga acaggtcgtc gtcggtgagg ctcgtcagga
     6301 tggtgacggc gagaaggacg gtgcccgccg gcttggcgcc ggcggcggcc tcgagcatcg
     6361 cgcggccgcc gagcgcgtgc acgttcacca tcgccggcgc gagcggcgcc agcacgcgca
     6421 tcgcacgctg gacggtggtc gggatgtcgt gaagcttcag gtccaggaag acgggcccgt
     6481 ccacggcgcg cacggcatcg tggccgttcg cgttccacat ctcgagtccg atcttgtaga
     6541 ggccggccgc gtcgccgatc tgcttggcga gcgccgaggc ctcttcgacg gtgggtacgt
     6601 cgagcgcgac gatcagtgga ttggtcatcc ttcgagcttc acgcgtcctt tcatgtcggc
     6661 gatgcggtcg atgccgtgtt ggccgcacca ggtgtggatc tcggccgcga tgcgctgcgt
     6721 ggcgcgcgga tcgtagaagt tcgcggtccc gacctgcacg acggtcgcgc cggcgacgat
     6781 catctcgatg gcgtcgcggc cggacgcgac accgcccgcg cccaggatcg gaacgtgcgg
     6841 caaagcgtgc gcgacctgga agatgcaacg aaccgcgatc ggccggattg ccgggccgga
     6901 caagccgccg gtgccggtgc tcagcttcgg acggccggtc gcgatatcga tcgacatgcc
     6961 gagggtggtg ttgatcagcg tgatgccgtc cgcgcccgcg tcggcgacgg cgcgcgcgat
     7021 ggcgaccaga tccgtgacgt cgggcgagag tttgacgaag ataggcgtgt cggcgacggc
     7081 tttgacggcg cgcgtgacct cggccgcgcg ggttgcgtcg agcgcgaaca tgtagccgcg
     7141 ctcttccagg ttcgggcatg acaggttcag ctcgacggcg accttcggcg cgccggcgag
     7201 ccgttcgacg accgcgacgt actcgtcgac gctgtggccg ccggcggacg ggatcgcggg
     7261 gacgccctgc tgcgcgagcc aaaccaggtc ggtgtcgcga aagacgtcca cgcccgggtt
     7321 ctggaggccg atcgcgttga gcatgccgct gggggtctcg gcgccgcgtg gcggcgcgat
     7381 gcccgcgcgc ggtctgagcg tgaccgtctt gcacacgacc gcacccatcg cgtgcagatc
     7441 ggtgaagcgc gagatctcct tgcccgagcc gaagcacccg ctggcgctga gcacggggtt
     7501 cttcagcgag atgcccgcga gatcgacggt caggtctacg gccatgcgat cacgctcccg
     7561 tcgaagaccg ggccgtcggt gcaggcgcgt tgatgatgcc ggtagccctc gtcgtgggtc
     7621 ggcacgacgc aggtccagca cacgccgacg ccgcaggcca tgaactcctc gaccgcgacc
     7681 tcgcagcggc ggccgttcgc gcgggccgcg accgcggcga gcatcggcat cggcccgcac
     7741 gcgtagatcg catcggcctc gatgccgtcc atcgcgtcgg tgacgcggcc gcgcaggccg
     7801 gtggaaccgt cgtccgtggt gatcgtgagc gtgtcggccg cgttggcacc acggtcgggc
     7861 gcgaacaatc ggcccttcgt cgccgccccg agaatcatgt ggctcgtcgt gccttgcgcg
     7921 cgcagcagcc gtgcgaggaa gaacagcggc gcggcgccgt acccgccgcc gaccagcaaa
     7981 gcggacccac cgggcgccgg catggtgtac gcggtgccga gcggaccgac gacgtcgagc
     8041 cgatcgccgg gcgcgctggc ggcgatgcgg cgcgtgccct cgccgatggt gtcgaagccg
     8101 atctcgactc gatcgccgtc cacgtcgagg atcgagaacg gccggcgcag caggcatccg
     8161 aacgcgtcct gcgcgacgtt gacgaactgt cccggcgccg ccgacgccgc gatctccgcc
     8221 gcttcgaaag cgaggatggt gtaggggccg tcggtgcgga cggacgcgac ccgcgcctga
     8281 gctatcgtgg ccgcggtcgt catcggtttc gtatcccccg aggttcgagc gcgtggggaa
     8341 agaagggcaa aagaaaagcc cgcgtaccta cgcaggccgg atcgtgcagc gtcaagctcg
     8401 tctctccctt ccgggcctca caggacccgc ccttaaaggg tgtacgacgc gagtatagcg
     8461 cgggcatcgc gccgccgttc gggcgccggg atccgacgcc cgaacggcgg gccggcgact
     8521 acgcgcccgg aaggggctgg tggtgaagca cggtcgccgt cggggcctcg ccgagcgtcg
     8581 ccgcgcctgc cacgaacgca cgggtctcgg cgtggaagac gcctgcgagg gcgaagtcgt
     8641 cgccggcgaa tgcgatcacg atcgctacgt cgccgcaccc gcccaccctg cactgcgcgt
     8701 tctccggtgg cgacggcggg ttgttcggat cgggcggctc gggcagcgcc ggcggaaccg
     8761 tgaccggcgg aactgggttg gggctcaggc agccttgcgg gccgaactgc agcaggaacg
     8821 gaacgaatgt gggcgaggtc gggtcggcac acgcgggatg cagcgacggg agcgccggcg
     8881 cggccggggc gcacgcagcc ggatccccgc tcggatcgca gggaggctgc ggagcgaccg
     8941 gcaagggcac gatctgcatg tcgggcgcgt gcctgttgcc gctgtcgtag gagtccgaca
     9001 cgaaccggaa caccttcagg tacggattca ccgagacggt gcccccgccc aggtcatcgg
     9061 tgaacgccat gaagttctcg agtcgcacgg taatcgtgga aagcacgcgc acggttgtcg
     9121 gacacagcag ataccagggc cctcggtcgc gggcgacgct ttggatccgg tacagaccat
     9181 tggcaagctc cgggcgcgag tcccacgtcg tgctccacgg acccgcgctg cctttgctga
     9241 tgttgagcac cgtcgcgttg tcactgagac gcgtgaccgt gatggtggag ttggctcgcg
     9301 cacccgcaaa cgcgcagacc gagtcttcga ctccgccgcg gttttcgtga atcaacactt
     9361 cgccgcgcac ggtctgtccg tcggccggcg tagtgatcgc agcctgcgca ggcgcgccgc
     9421 cgagcacggg aagggccatc gccgtcgcca gccacgccgc ggcgaagcgg acacgccggc
     9481 gtttcccgaa atcattcatc ccagtctcct cactcggtcc gtcctacgga atgtcgggca
     9541 atccggccga gatgccgagc accggatggt tatcgaaaat cgcggccccg ttcgacaggg
     9601 ccagcgcgcc ggcgcccccg cagagcgtgt aaccgaacgc gtccagacgc gcagacgcgg
     9661 aaaagaacag gccttggccg acgaagtcga tcgagcgatc ctcggcgaag gaggccccgg
     9721 aaaccatcgg cagccagacc ggatagggag cggccgcggg ctgcgccgtc gacacggtgc
     9781 ttccgatcag cgtcccgacc tgatgcaacc cgagcgtgta ccgggccgtg atatgcatcg
     9841 tcgggcccag ccaggcgtac gccggaagga tccacgacgc gggcgggatc accgggctaa
     9901 gcaggccggg gaccggcgtc aggaacagcg gcgctccggt cacgggaacg atgggcggta
     9961 ccggaacgcc ggcggcgccg ccggcctggg acgcaagcgc gtaggtgtcg ggcacggttt
    10021 gcgacctccg gacggagacg ctctgacagt tcgcgacggc ttcgctggtc gcgccggccc
    10081 acagagtggc gaacaggtgc gtgatgttcc ccgccgcgta ttcgggcgtc ccgagcggcc
    10141 cttgggccat ggccgaggag gcggcggtgt ccgggtagag gttgcgcagc acctcaccga
    10201 acgggcgaac gtattcacgg acctcgccat cgccgtgcac cgagatggtt gccggcgcat
    10261 tcagcagcgt ctcgaggtcg acaccgaact cggcggcctt ctgctcgaac attgcacgaa
    10321 agcgctcggg cacggtctgg acgcgggttg cgttcccggc ggtggtggtg gcgagcagcg
    10381 ctgcggcaac cgccgtcagc aggaaccgtc tcgtcgaagt cattcccctc cccttcccga
    10441 tcaacagaag tagccggaaa cgccgatctt gatgttgagc agccagacgc tcagcgagcc
    10501 gcccgagcag ttgacgtagt gcttcttgtt gcacgagaag atgtaggcgc accaaccgcc
    10561 ctgacgctga tcggcccaga agttcccgtt gccgaaggtc tgctccagac cggtgcctgc
    10621 ggactcgtca cgctggcgga tcgtgccgcc caggatccgg tgatgacagg ctccggagtc
    10681 cgactgctgc ggtgtcgcag cgcatcccgg cccgtggtcg gtggtgttct cccagatgta
    10741 gccgcttgag tcactgccac cgaactcctg ctcgaagaat cgcacctgcc agttttggat
    10801 caggaagtac ttcgaggcac cgctctgcgt gcagctcgcg ctgagcggcc gctggcagtc
    10861 gaagatgttc acggtcacgt gctcgctggc atggatcgag ctcgacgccg aagggctttg
    10921 tcccgacggg cgagtgtcgg acgcagggcg gttcgagcac gcgccgctgt tcgccgagca
    10981 gaaatccagg tgcgtgggca gcggcatgat gctcgttgac atgttccagc gatcgcccga
    11041 gttcgactcc atcgtcaagc cgttcgtgcg ctgcgccgcg tcgtaggtca tcagcgaccg
    11101 gccccccaca tccaccatcg aaacgacgat gttgggcacg agatggtcga gcgtgacgcg
    11161 caggtactcg agcttggcgg tcgccgtggg acgacgctcg atatccacct tgaagatctt
    11221 cgtgctgagc ccgtcgatcg agaagttcgg agccttcgcc gatgcccgtt tgatttgagt
    11281 gagccgcgcg tggaccacgt agcgcgcgcc ggttccggcg atgtccctta gcagcatgcc
    11341 gtcgaactgt gcgtcgatcc ggtcgccgtt cgggatgctc gacagctgga actcgagaag
    11401 gccgagcacg gagccctttg catcgaccga gacggtgccg tcggcgtcga agccgacatc
    11461 caccgcggtg ggcaggcttt cgaggcgcgc atgcaactcc gtcgcgcggc cggcgatccc
    11521 gtccgggtcg aaggcgtcga tggtgatcag cgcgaccggc tgcgaagcgg tgtaggtgaa
    11581 cttggtgtcg ctgagaaacc gggcggacag ctcggtcggc aggatttgga tcaccgcgtc
    11641 ggtcgtggcg cggccgccgg gcttgtcggt gttgagatcg atttggaact cacggccgcc
    11701 gacggtgttg agctgaagtg ccgggggcgg gccctgctcg accaacacct tgcgcagccc
    11761 ggtcacccgg gcgaacagcg cgtagtggct gctgagatcg ttgagcagaa tgccgtcgac
    11821 ggactcgggg agccggtcgt cgggccccga ggtcagctgg acctcgatca gcccgagtgt
    11881 gtttcccctc gcgtcgagtc cgatcgcgct ccccccgtcc ccgaacgtca gatccatgct
    11941 cgtggggata tcgcgcatga gcagatgcag cgcggtggcc cgtccggaga tgcccgccgg
    12001 atcggtcgca tcgaccgtca attccccgat gggcgccgag cccgcgtagg tgatcgtccc
    12061 ttgcgtggag gagaagccga ttcggaactg ggaaggcagg tcgcggaccc acgcgtcgat
    12121 cgtctgcgtg ccttgcttgg tgagcacgtg cagcggaccg ctcgccagac gcgcatccag
    12181 cagagatggg tcgcccgttt cgagctcgaa ctccgatagg ccgagcacgc ggaccgcgag
    12241 cgagtcaacc ccgcccgagt cgtcggtata caggtaatgc ccctgttcgt cgagccatcg
    12301 gatggcgccg cctgtggcgt tgccgatctc gacgaggccg atggcatccg aggcacgaaa
    12361 caccgaatgc gtcggcgagt cctgcacgag cgtggcgcgc agcgccatgt cctggatctt
    12421 cagatggaat tcgctctgca ggacgtcgct gcggctgtca ctgatgcgaa gatcgatgct
    12481 ctgcacacga gccgtggcgg tgtagtccca cgttcgagtg ccgagggggg agtcgttcag
    12541 gaccagcgac atcgttcctg ccggcagcgg gtcgatcgtc gcttcggcgc gcgtgtcgcg
    12601 cgtcccttcg aggctttgca gcagaacccg tgcggttgtg tcgatgttcg acgtcaagtc
    12661 gatcgaggtc tgcgtcacgc cgaagtcgga tccgaccaag ccggcgaagt gagacagagt
    12721 cggcaccggc gcataggaga cccggccacg gaccgggtcc tctcgctcgc ccgacgaacc
    12781 ttcactgaag atctctgcga tcaccgcgag cgtcggtccc gccacggcgg tttcgacgtc
    12841 gagcgaaaag cttgccgcgc ggccggcacc ggcgaggaag atcgtggcgc tatagaccgc
    12901 gggcgcgacg gaatcgagcg tgtcgtatcc gaaagcgacg cggtagggac ttccttgtcg
    12961 cgggtcgcgg agcacggcct cgatcagaag cggcagttcc gcgcgcgcgg acggaagctt
    13021 ggtcacccgc agcgtcgcgc gagcggcatc gatctgcagc tgggcggcga catcggggat
    13081 cccgtcaccg tccacatcga cgagcgaggg cacgagcagg atcgcgtcgg ccgatgtttc
    13141 gacgcgtgca ccttccgcgt cctcggcgac ggtgtggatc ttgtacgaga gcagatcgag
    13201 aatctcgctt cgtgccaggc cgggggcttg caccgacaag gccatgatgg ccgcgagcag
    13261 caatgaaacc cgtcgcactg ctctgtgtga tgcgccgatc gatttccccg tcctcatctg
    13321 cattcccgcc cttcctggtg gttacgaatt cctggcaatg ccgtgctcat gggagcggcg
    13381 gcgcggtctg cgcctgcggc gcgtagcgcc gcactccgtt cgtggctgct gcccacatca
    13441 cgccgcccgg ctcgacggca atgcccgccg ccgcgccggc ccactgctcg agcaacgatc
    13501 ccgtgcgcga gaaatgggag agccgctgat tacccgaatc ggaaacgaag acaccgtagg
    13561 tggtcgcgac gatcccgagc ggggccgaca tgcagccgtc ctgcacgcgc gaaccacccc
    13621 agaacccggc cggctcgccc gcgatcgtga agcgctgaac tctgttgttg cccgtatcgg
    13681 cgacgaagac ctgtccgcga tagaccgaga cggccttcgg atccgacacc tgcccgggat
    13741 ccgacccgga tgctccgatg acggcctgcg gcgctccggt cggcgacacg atttggatcc
    13801 ggtcattgcc gctgtcggcc acggcgatgg acccggcaag ggggccgtcg gggacaatcg
    13861 cgatcccgtg cggcgagctg aacgcgccgg gcgccgcgcc ggctgtgccg aactgcgcga
    13921 ccttggcgat gccgaacgaa tcccagacct ggatccggtc gttgcccgtg tcggcgatgt
    13981 agacgaatcc gttcgccgtg acggcga
//
LOCUS       DATLFO010000019         6234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_39982, whole genome shotgun sequence.
ACCESSION   DATLFO010000019 DATLFO010000000
VERSION     DATLFO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39982"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VM841_00695"
     CDS             <1..261
                     /locus_tag="VM841_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVL88729.1"
                     /translation="ADQAMDFVAAIRRHRHVVSVHPSRRHLDVFEELCRRTRATGNLV
                     PDAYLAAIALETGSTIASADSDFRSFAGVRWINPLSRSAKKA"
     gene            complement(577..1629)
                     /gene="pseI"
                     /locus_tag="VM841_00700"
     CDS             complement(577..1629)
                     /gene="pseI"
                     /locus_tag="VM841_00700"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HVL88730.1"
                     /translation="MHIEIAGRRIGPGEPVYVIAEMSANHLGDFDYAAKIVHAAAQAG
                     ADAVKLQTYTADTITIDVDSPLFKERTGGTLWEGRGLYDLYQEGSMPWEWQPKLKAIA
                     DGLGIHLFSAPFDDSAVDFLEAEVNPPAYKVASFEIVDIPLIEKMAATGKPVLISTGM
                     STKDEIQEAVDAARGAGATQILILKCTSAYPAPPEEMNLATIPQMIKDFGVPVGLSDH
                     TMGIAAPISAVTLGASLIEKHFTISRADGGPDGAFSLEPAELAEMIEGIRLAEKAVGR
                     PAYGPGTAETGSRVFRRSLFVVEDVKAGDPLTQRNVRSIRPGDGLHPRHYKEVLGRIA
                     KRDLPRGTPLGFDDLQ"
     gene            1784..2869
                     /locus_tag="VM841_00705"
     CDS             1784..2869
                     /locus_tag="VM841_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVL88731.1"
                     /translation="MSTAALRSAWTLIRLTARSAREDRITAIAAQLAFFALLAIPSAL
                     LLLAGTAGYVDRLFGSEAGAALKEQVETALGSFLDPDAMRSFVQPIVDDLFAGGRADL
                     LSIGALVGLWSASRAVRVLIESMNIAYDIDDWRPAWKRRLIAIGLSVGGLIAMAVIVP
                     LMLAGPGVGEALVRRLGLPPAFGSTWRILYWPVAALVVVGGLATLYHIAPNWRTPWTR
                     DLPGAFLAAAAWVAAAWGLRVYVSRAISETTAGPLAAPMILLLWLYVSGLAVLLGAEL
                     NAEIERMRQARNAQRRGPAARLLERFGSRRARRTREEAEADQQDAAAEADPPADPEAA
                     GGLIERGAHPAGDPGRTEPGRERNTNP"
     gene            2866..4362
                     /locus_tag="VM841_00710"
     CDS             2866..4362
                     /locus_tag="VM841_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88732.1"
                     /translation="MTRRAWIVGLLAALAAALPAAASHNADDHSDNMSLVANWDHDGT
                     YRQGSDIAFWGDTMVLGNFDNPGGFRVIDIANPAKPKLLGHFKCPGPQSEVAIWRDLV
                     FVGVDNGRAGESCDEAGQSSAAARLTGDSWEGIRVVSIADPAKPKQLAAVHTDCGAHT
                     LTLIPDTAKDRVILYANSYPAGRQGAGCNAHFKISVVEVSLSKPQDAKVIATPSVGTA
                     VGCHDITVHMERKLAAAACLTETQIWDITDPVAPATLGTIRNASMNIQHSTAWSNDGK
                     KLVIGDEMGAAAAATGCMAEGHLPLGALWFYDIAEPSSPQMRSSLVIPRREVSIFCTA
                     HNFAPVPMIEGRDILVSGWYNGGTTVLDFTDPSNVEEIGHYTPRTGPKPASWASYWYR
                     GHIYVNNFDEGFIYEAGRSRGLDVLKMSDPRVAKALTLTHLNPQTQEALRAAAPAPEP
                     KPKPVKVLPVKQTRAPLPATGVGAAPSAALLLALAVAAAATLAKPRTR"
     gene            complement(4359..4826)
                     /locus_tag="VM841_00715"
     CDS             complement(4359..4826)
                     /locus_tag="VM841_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL88733.1"
                     /translation="MTEVRFRAATAGDCRLIFDWANDPQTRANSAVTDRIEWDGHVEW
                     FEHKLADPNAEIWIVENGEGMPMGQVRFDVNGGEAEIGIMVAAEHRGGGLGTRMIEQA
                     TRDFLARRPGVTVIAHVRHHNDVSQRAFVKAGYEPDGEGEARGVTMLRFVRRG"
     gene            complement(4898..>6234)
                     /locus_tag="VM841_00720"
     CDS             complement(4898..>6234)
                     /locus_tag="VM841_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88734.1"
                     /translation="SQDVIVRTEDCKTKEHIDMAVLRDGEPNPNLVGRFTAAKVVTKR
                     GRELLGKNAPITKAMLAEIVEGLGEEPGATVPVRSVLKCEAEGVCQHCYGVAPATGKL
                     VAIGDAVGIIAAQSIGEPGTQLTMRTFHLGGVAGLDITHGLPRVVELFEARKPKGEAQ
                     IVELTGSVEIIEGDKAREIKVTSEDGKEEVVYKVPGRAKLRVKTGDTVEVGQQIAEGS
                     VNPHEILRIQGPQAVQLFLVGEVQKVYHSQGVTIHDKHIELIVRQMLRRVNVLEPGDA
                     EFLPGQLVERKAFEGVNRRVVGDGGDPASARPMLMGITKASLATDSWLSAASFQETTR
                     VLTDAAISAKSDPLLGLKENVIIGKLIPAGTGMARYRTIKVFPTEMPERLYPVGADDE
                     SPLMEEGARPFEMPVPPQFAVPPEEAYAEGAAAAAAAEGDGAPLGDVSEAGE"
BASE COUNT          945 a         2231 c         2039 g         1019 t
ORIGIN      
        1 gccgaccagg ccatggattt cgtggcggcg atccgtcgtc atcgccacgt cgtttccgtc
       61 cacccgtcgc gccggcacct cgacgtcttc gaagagctct gccggcgaac tcgcgcaacc
      121 ggcaacctgg ttcccgacgc ctacctcgcc gcgatcgcac tcgagacggg atccacgatc
      181 gcaagcgccg attcggactt ccgctctttc gcgggggtcc gatggataaa ccccctcagc
      241 cgctcggcaa agaaggcctg agagatccag gcaatgaccg tcagatgtgg ccgctcgatc
      301 aagagccgca gatgaatctc gtcgtttcga agctccacca tgacgagcgc caagccttga
      361 ccaagaccca tgcttgtcgg tgacgaggag gtcggccgtc atccaattcg aatcctcggt
      421 ggtcgcgact gcaactctgc caccgtcggg cgacgccgcc atcgctcgca ggctcgcgcg
      481 gtagaagaac gcgtccgatt caaaggcccg gccgcgagat tctcgcgacc ccatcatcga
      541 gcggaatcag agctcccaga atcaggtcgc acgtccttac tgaaggtcgt cgaaacccag
      601 tggggtgccg cgcggcaggt cgcgcttggc gatgcggccg aggacttcct tgtagtgacg
      661 gggatgcagc ccgtcgccag gccggatcga gcgcacgttg cgctgggtca ggggatcgcc
      721 ggccttgacg tcctccacga cgaagaggga gcggcggaac acgcggctgc cggtctcggc
      781 cgtgcctggc ccgtaggcgg gccgtccgac ggccttctcg gccagccgga tcccttcgat
      841 catttcggcc agctcggccg gctcgagcga gaacgctccg tccggcccgc cgtcggctcg
      901 cgagatggtg aagtgctttt cgatcaggct cgcgccgagc gtgaccgccg agatcggcgc
      961 ggcgatgccc atcgtgtggt cgctcaggcc aaccggcacg ccgaagtcct tgatcatctg
     1021 cgggatggtg gccaggttca tctcctcggg cggcgcggga tacgccgagg tgcacttcaa
     1081 gatcagaatc tgggtcgcgc ccgcgccgcg ggcggcgtcc acggcctctt ggatctcgtc
     1141 cttggtgctc attcccgtcg agatcaggac cggcttgccg gttgcggcca tcttctcgat
     1201 cagcgggatg tccacgatct cgaaggaggc gaccttgtag gcgggtgggt tcacctcggc
     1261 ctctaggaag tccacggcgc tgtcgtcgaa gggcgcgctg aacagatgga tgccgagccc
     1321 gtccgcgatg gccttgagct tcggctgcca ctcccacggc atcgagccct cttggtacag
     1381 gtcgtacagc ccgcgacctt cccacagcgt gccgcccgtg cgttccttga acagcggcga
     1441 gtccacgtcg atcgtgatcg tgtcggccgt gtaggtctgc agtttcaccg cgtccgcacc
     1501 ggcctgggcg gccgcgtgca cgatcttggc ggcatagtcg aagtcgccga gatggttcgc
     1561 cgacatctcg gcgatcacat agacgggctc gcccggcccg atccgccgtc ctgcgatctc
     1621 gatatgcatc gcccgaaagc ctagcgcggc tcaggaccct tcggtcatct cgcgcagcgc
     1681 gcccacgatc tcgagccgga cccgtccttc ggccggcacg cttcattcag gtcaggtcgt
     1741 cgagcccggg ggcattcgct ggcggggccg taggctgaag gctatgagca ccgcggcgct
     1801 tcgctcggcg tggacgctga tccggctcac cgcgaggtcc gcccgcgagg accgaatcac
     1861 cgcgatcgcc gcccagctcg cgttcttcgc gctgctggcg atcccgtcgg cgctgctgct
     1921 gctggccgga acggccggct acgtggaccg gctgttcggc tcggaggccg gggccgcgtt
     1981 gaaagagcag gtcgagaccg cgctggggtc cttccttgat cccgacgcca tgcgctcgtt
     2041 cgtccagccg atcgtggacg acctgttcgc cggcggccgc gccgacctgc tctcgatcgg
     2101 cgcgctggtc ggcctgtggt cggcctcgcg cgcggtccgc gtgctgatcg agtccatgaa
     2161 catcgcctat gacatcgacg actggcggcc tgcatggaag cgccggctga tcgcgatcgg
     2221 cctgtcggtc ggcggtctga tcgcgatggc ggtcatcgtg ccgctgatgc tagccggacc
     2281 cggcgtgggc gaggcgctgg tgcgccgcct cgggctgccg ccggcgttcg gatcgacctg
     2341 gcggatcctg tactggccgg tcgccgcgct cgtcgtcgtc gggggcctgg caacgctcta
     2401 ccacatcgcg ccgaactggc ggaccccgtg gacccgcgat cttcccgggg cgttcctcgc
     2461 cgcagccgca tgggtcgcag ccgcttgggg gctgcgcgtg tacgtgtcgc gcgcgatcag
     2521 tgagacgacc gccggcccgc tcgccgcgcc gatgatcctg ctgctgtggc tctacgtcag
     2581 cgggctcgcc gtgctgctcg gcgccgagct caacgccgag atcgaacgga tgcggcaggc
     2641 cagaaacgca cagcgccgcg gccctgcggc ccgcctgctg gagcgattcg gctcccggcg
     2701 cgctcgccgc acccgcgagg aggccgaggc cgaccagcag gacgccgcgg ccgaggccga
     2761 tcctcctgcg gaccccgagg ccgccggagg gctgatcgag cgcggcgccc atccggctgg
     2821 cgaccccggc aggaccgaac cgggccgaga gcgaaatacc aacccgtgac ccgtcgcgcg
     2881 tggatcgtcg gcttgctggc cgccctggcc gcggctctgc ccgcggccgc ctcgcacaac
     2941 gccgacgacc actccgacaa catgagcctg gtcgcgaact gggaccacga cggcacctac
     3001 cggcaaggca gcgacatcgc cttctggggc gacacgatgg tgctcggcaa cttcgacaac
     3061 ccgggcggct tccgcgtcat cgacatcgcg aacccggcca agccgaagct gctcggccac
     3121 ttcaagtgtc ccggcccgca gagcgaggtc gcgatctgga gggacctcgt cttcgtcggc
     3181 gtggacaacg gccgcgcggg cgagtcctgc gacgaggccg gccagtcctc ggccgcggcg
     3241 cgcctgaccg gtgacagctg ggaaggcatc cgcgtcgtgt cgatcgccga tccggcgaag
     3301 ccgaagcagc ttgcggccgt gcacaccgat tgcggcgcgc acacgctgac gttgattccg
     3361 gacacggcca aggatcgcgt gatcctgtac gcgaactcgt atccggcggg ccgccagggc
     3421 gccggctgca acgcgcactt caagatctcg gtcgtcgagg tttcgctgtc caagccgcag
     3481 gacgcgaagg tgatcgcgac accgagcgtc ggcaccgccg tcggctgcca cgacatcacg
     3541 gtgcacatgg aacgtaagct tgcggccgcc gcatgcctga ccgagacgca gatctgggac
     3601 atcaccgatc cggtcgcgcc cgccacgctg gggacgatcc gcaacgcgtc gatgaacatc
     3661 cagcattcga ccgcatggtc caacgacggc aaaaagctgg tgatcggcga cgagatggga
     3721 gccgccgcgg ccgcgaccgg ctgcatggcc gaaggtcatc tgccgctggg ggcgctctgg
     3781 ttctacgaca tcgccgagcc gtcctcgccg cagatgcgct cgtcgctggt gatcccgcgc
     3841 cgcgaggtct cgatcttctg caccgcgcac aacttcgcgc ccgttccgat gatcgagggc
     3901 cgcgacatcc tggtgtcggg ctggtacaac ggcggcacga cggtgctcga cttcaccgat
     3961 ccgtccaacg tcgaagagat cggccactac acgccgcgta ccggcccgaa gcccgcctcg
     4021 tgggcgtcct actggtatcg cggccacatc tacgtcaaca attttgacga gggcttcatc
     4081 tacgaagccg gccgaagccg cggcctggac gttctgaaga tgagcgaccc gcgtgtcgcc
     4141 aaggcattga cgctgacgca tctgaacccg cagacccaag aagcactgcg cgccgccgcc
     4201 cccgcgcccg agccgaagcc caagccggtg aaggtcttgc cggtcaagca gacccgcgcg
     4261 ccgctgcccg caaccggggt cggcgccgcg ccgtccgccg cgctgctgct cgcgctcgcc
     4321 gtcgcggccg ccgccacgct ggccaagccg cgaacgcgtt agccgcgccg cacgaaccgc
     4381 agcatcgtca cgccgcgcgc ctctccttca ccgtccggtt catatccggc cttcacgaac
     4441 gcccgctgcg acacgtcgtt gtgatgccgc acatgcgcga tcaccgtcac gcccggccga
     4501 cgcgccaaaa agtcgcgtgt cgcctgctcg atcatccgag tccccaggcc accgccgcga
     4561 tgctcggccg cgaccatgat cccgatctcg gcttcgccac cgttcacgtc gaagcgcacc
     4621 tgacccatcg gcatcccttc gccgttttcg acgatccaga tctccgcgtt cggatcggcc
     4681 agcttgtgct cgaaccattc gacgtgcccg tcccactcga tccgatcggt caccgccgaa
     4741 ttcgcgcgcg tctgcggatc gttcgcccaa tcaaagatca gccggcaatc gcccgccgtc
     4801 gcagcccgga atcgcacctc cgtcatcgcg cggaatcgta gcggcaaagc gtcgagcccc
     4861 tgggaggcga cccaggggct cgacagaaca ggcgttgcta ctcgccggct tcggaaacgt
     4921 cgcccaacgg cgcgccgtcg ccttcggccg cggctgctgc ggctgcaccc tccgcgtagg
     4981 cctcctccgg cgggactgcg aactgcggcg gcaccggcat ctcgaacgga cgggcgccct
     5041 cctccatcaa gggcgactcg tcgtcggcgc cgaccgggta gaggcgctcg ggcatctcgg
     5101 tcgggaagac cttgatggtc cggtagcgcg ccataccggt tccggccggg atgagcttgc
     5161 cgatgatgac gttctccttc aggcccagca gcggatcgct cttggccgag atcgccgcgt
     5221 cggtcagcac gcgcgtcgtc tcttggaacg atgccgccga cagccacgag tccgtcgcca
     5281 gcgacgcctt cgtgatgccc atcagcatcg gacgcgccga cgcgggatcg ccgccgtcac
     5341 cgacgacgcg ccggttgacg ccttcgaacg ccttgcgctc gaccagctgg ccgggcagga
     5401 actcggcatc accgggctcg agcacgttca cgcggcgcag catctgacgc acgatcagct
     5461 cgatgtgctt gtcgtggatc gtcacaccct gcgagtggta gaccttctgg acctcgccga
     5521 ccaggaacag ctgtacggcc tgcgggcctt ggatgcgaag gatctcgtgc gggttgaccg
     5581 agccttccgc gatctgctgg ccgacctcga cggtgtcgcc ggtcttgacg cgcagcttcg
     5641 cgcggcccgg caccttgtag acgacctctt ccttgccgtc ctccgaggtg accttgatct
     5701 cgcgggcctt gtcgccctcg atgatctcga ccgatccggt gagctcgacg atctgcgcct
     5761 cacccttggg cttgcgggcc tcaaacagct cgacgacgcg cggcagaccg tgcgtgatgt
     5821 cgagcccggc cacaccaccc aagtggaagg tacgcatcgt aagctgcgtg cccggctcgc
     5881 cgatcgactg ggcggcgatg atgcccaccg cgtcgccgat ggccacgagc ttgccggtgg
     5941 ccggcgcgac gccgtagcag tgctggcaca cgccctcggc ctcgcacttc aggacggagc
     6001 gcaccggcac cgtggcgccg ggctcctccc cgaggccctc gacgatctcc gcgagcatcg
     6061 ccttggtgat gggagcgttc ttgcccagga gctcgcggcc gcgcttggtg accaccttgg
     6121 cggccgtgaa gcgccccacg aggttggggt tgggctcgcc gtcgcgcagg acggccatgt
     6181 cgatgtgctc cttggtcttg cagtcctccg tgcggacgat gacgtcctgg gaga
//
LOCUS       DATLFO010000020         9642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_59868, whole genome shotgun sequence.
ACCESSION   DATLFO010000020 DATLFO010000000
VERSION     DATLFO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59868"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VM841_00725"
     CDS             <1..1321
                     /locus_tag="VM841_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HVL88735.1"
                     /translation="STLLPDGTVGTLLKGPVFTLYAAGGWLIVRGDVAALRALPQIPA
                     EGGGSRRVVLAAATTTQARTMVAVAPALRAAGFEPSFVSLDPIVGKNAAYGFEEAGVE
                     HTALGTPRATQRGRMLHVVFGGRGEARRLVDGAAAVVVGNDFTPFERLIVQEARRAGV
                     ASVLLQDGVIALGGDQAGGGARSGSRPVTLAKRALSLLGLPFSPKPYGMGGCDAVCVY
                     GPSTAEALTRRGVAAASIHVTGQPRYDTAAGASAAAKHTVRRDGSIVVTTQPFSRYGL
                     ADPAEETALFAAMIAAAAERGPVIVKLHPDTDASSRAALQAAAPGGIRFEQQIPIEQL
                     LATARLLVTLSSTTALEAMLHRIPVVVVDAPGFPTTLPYIEAEAVESARTPAEIGAAI
                     DRALAGDLRASYRERFLAAHLAPLDGRAAVRVAAAVLAAVATKRESL"
     gene            1398..3284
                     /locus_tag="VM841_00730"
     CDS             1398..3284
                     /locus_tag="VM841_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL88736.1"
                     /translation="MNAPDRATPSEAAAAIVSLISDAKFRVLALSVTGLLLLWFVAAA
                     VWVAMPVWLLVAAWWYRHARTDDWLVLALAGLPFWWATGLAQYAIPALAGLGVIGAIR
                     AEPLRGRIKDAWESLGLEKWLALGAFLSMIPSVLVVSEPGRIFTYIRTVGLTGVALAG
                     LLVASAWSRKHDARRLLHFLVGFAGVVGLWTLAQVVYKGSIHLPLQRTASLLEWLPGK
                     AGELTSRAVGEAAGIKYENVGGGRLVVRPHAFFIHAVVTGAMSALLGPLIWRRIRHVP
                     VWRAVAAAFFAGLTGIMLVSSLARGAYLGVAITVPLFAVIFWPDIRRVAPKVLAGGLA
                     IAIAVSGIVLLTRGGEARARQSSQLAEGSFVSRAAYYRATLRAIPQHPLFGHGTQRDN
                     VAAPLQVPDGTRILEIPKGTTVSVYGDGSGGHYLLHDLPAEPDRRIHVPSDRTVAAPE
                     TGDFRAPDGAHADVLADGGEEAIVSNAERGIIVVRKFPSGRQERIYFSNDAAQQARLI
                     STPDRDPPLGSHSTLLGVGYKYGILGLLAFVALWAYTGLAPLRTLRGGTRPLFDDARA
                     LWFGITAFGAQFLIYEYDFDSSSPVIFYLLSGLLIGLSARPARVELSPEPDSPAEPAR
                     ATSR"
     gene            3281..4774
                     /locus_tag="VM841_00735"
     CDS             3281..4774
                     /locus_tag="VM841_00735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HVL88737.1"
                     /translation="MTTTARTPATDAPSAKAPAPASVWRRRWLVLVLLADLVSYAIAV
                     FGAYQIRFGAGEPAYMIVNLLGDNRYRLAYVPAFAIMLPVWLGVLFFNRCYSWRHVSY
                     PPFELRRVLSALTMGVAMFGLFSFGFRANINRPLLLVLFGVATITVPAGRLVARNIWK
                     RWRRTAGARARTLIVGANREGRIVADIMIANPTFDFVPVGFVDDDPEPPSPDFVHDIP
                     VVGGTKEILDLVDSMTISAVFITDSTLGPERTGAILRALRPRDVEVRISANLPEILAW
                     RLAIEPITGFPMLSVDNGKMERFNRFAKRTLDVAGSAAGLLVLSPLLIGLAIAIRVDS
                     PGPVFFRQLRAGYKGKPFKVFKYRTMVNDADKIKLSGEIASEVGGPIFKVRDDPRITR
                     VGKLLRRYSLDELPQLINVLIGQMSLVGPRPLPLHEQEELDRIAPERLEVRPGITGLW
                     QVSGRSDAPTERLVRLDLFYVENWSIAYDLYVLYKTIPAVLKKSGAY"
     gene            complement(4881..5642)
                     /locus_tag="VM841_00740"
     CDS             complement(4881..5642)
                     /locus_tag="VM841_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HVL88738.1"
                     /translation="MTYPDGISRVRVLGVDIAQVTRAQLERYGIEALDREGTTLLYAI
                     HVVGVNILGSDPDYERALANADLVHADGTSVLMTVRAAGHTLPERIATQDFMYQVIEH
                     CVAHGHSIFLLGGEPGLAQRAADELARRYPGLTVAGSRDGYFTDAEGGEVAARIRASG
                     AHLILVGMGCPREQIWADRHGRDTGVRLLMTVGGTFGYIVGEESRAPRWVQKTALEWA
                     WRLAQDPRRLAKRYLGGIPQLLRQMRRARRESRSA"
     gene            complement(5639..6964)
                     /locus_tag="VM841_00745"
     CDS             complement(5639..6964)
                     /locus_tag="VM841_00745"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL88739.1"
                     /translation="MRSSRRALPALLPLLLVLVVAAATCGQTGTVGFAGTVTAQARAS
                     MREPARGAGPLARVATGTFRKKLNTQAFDSGRATTHVSAMFEGLGMVGATRGGTLFAV
                     QRRGTSSIDPTGQLVFFEYRGAERGWTERPIYDSEWDDRNAAGGVTSTGTIVAFFARY
                     DSHTGIWHDMGFIRSTDDGQSWSDYKTVPVGADRWYSPYGKLVELPGGRLMQLLYGND
                     GYSYRVRAIFSDDDGETWGNEVTIDRQSFERPTEADAILVDGTTNDNARLIVVSRTEP
                     GPNPQAGGGLYQYVSANGGRTWKRMGFMNVGDTPSDISPWLTNLSDGRIALVWAARRT
                     MTIEVSIADGARVFRNRNGWGKPYTLFTSRLANQRPRQAANFGYPAIGTVGQSDEDQV
                     VIFFDANSSDPSVFHEKAFANTDLVMIRLLGPKIAERKRLEREAQHSSS"
     gene            7460..8905
                     /locus_tag="VM841_00750"
     CDS             7460..8905
                     /locus_tag="VM841_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HVL88740.1"
                     /translation="MTKTMNRGLRRTTVGTVALAMLATMMFVLVHEEPARADHMAVSE
                     GWDFSPAQEPFTAQKDTPKEFFIFLNQSVTTRTLIMMEIESGSSDPDQPTEANGFTPD
                     NSYSKPEAWCYINAGESTCTITYTFTVVPIIGVDNVIARFYINKHLIDTQEQPNESTA
                     NQSSLGCDGVFGGPEHENEPDTTEAAQIFVYGAIGGTAPNPVPPPPGVQASRTCENPG
                     GGGTPTQSPTQSPTGQPTQSPTGEPTQSPTGEPTQSPTQSPTGQPTSNPTNTGPRGGP
                     GGTFGEMTMSVSPNVGTYGTDFLISGEVVCHGVKVANARLQIYRHFVGSRSIDVIETS
                     TDVQGKYAFRDTDLRASADYQGMWNGRTGSTSACTSGAETGPDRRRADVRPGVIVNVR
                     DTTLPKGEAATFFGSVIPAHPGQKVFLQVYHGQQGRWEYATETRLDRRSNYSIRFSRN
                     ATGYLLFRIAYPTQDLDHSWNISRTVRVDWS"
     gene            9045..>9642
                     /locus_tag="VM841_00755"
     CDS             9045..>9642
                     /locus_tag="VM841_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88741.1"
                     /translation="MSMPNRPSWRPLAGATTVLLVLSAISFVVLRDQPARAAHSDATI
                     DVVNTAETVNGLPGGTVSWSVTAMKTNADFSQSPVAGLVILAEIEGGSNDPDGNVEGD
                     QTFGLSYGTPDLKCTTGSDGSCDIQFTPYALQGTAANVRIWIQGGVGIDTNEGLVETS
                     QAGCGTEPDGTEVVAFTSQTTSGTAPARAAPGAEPNPTV"
BASE COUNT         1473 a         3382 c         3252 g         1535 t
ORIGIN      
        1 gtcgacgttg ctgccggacg gtaccgtggg aacgctgctg aaggggccgg tgttcacgct
       61 ctacgcggcg ggcggatggc tgatcgtgcg cggcgacgtg gcggcgttgc gtgcgctgcc
      121 gcagatcccg gccgagggcg gaggatcgcg gcgcgtcgtg ctggccgccg cgaccaccac
      181 gcaggcccgc acgatggttg ccgtcgcgcc ggcgctgcgc gcggccggct tcgagccctc
      241 cttcgtcagc ctcgacccga tcgtcggcaa gaacgccgcc tacggcttcg aggaggccgg
      301 cgtcgagcac accgcgctcg gaaccccgcg cgcgacgcag cgcggccgca tgctccacgt
      361 cgtcttcggc ggccggggcg aggcgcgcag gctggtagac ggcgccgcgg ccgtcgtcgt
      421 cggcaacgac ttcacgccgt tcgagcggct gatcgtgcag gaggcacgcc gcgccggggt
      481 ggcgagcgtg ctgctgcagg acggcgtgat cgcgctcggc ggcgatcagg ccggcggcgg
      541 cgcgcggtcg gggtcgcgtc cggtgacgct cgcgaagcgc gcgctgtccc tgctcgggct
      601 gcccttctcg cccaagccct acggcatggg cggatgcgac gcggtgtgcg tctacggccc
      661 gtccaccgcc gaggcgctca cccgtcgcgg ggtggccgcc gcctcgatcc acgtcaccgg
      721 ccagccgcgc tacgacacgg ccgccggcgc ctccgccgcc gcgaagcaca ccgtccggcg
      781 ggatggctcg atcgtggtga ccacgcagcc gttctcgcgc tacggcctcg ccgatcccgc
      841 cgaggaaacc gccttgttcg ccgcgatgat cgcggccgcg gccgagcgcg gcccggtgat
      901 cgtgaagctg cacccggaca ccgacgcgtc ctcgcgcgcg gcgttgcagg ccgccgcgcc
      961 cggcggcatc cgattcgagc agcagatccc gatcgagcag ctgctcgcca ccgcgcgcct
     1021 gctcgtgacg ctatcgtcca cgacggcgct ggaggcgatg ctgcaccgca tcccggtcgt
     1081 cgtggtggac gccccgggct ttccgaccac gcttccctac atcgaggccg aggccgtcga
     1141 gtccgcgcga acgcccgccg agatcggggc ggcgatcgac cgcgcgctcg ccggggacct
     1201 gcgcgcctcg tatcgcgagc ggttcctggc ggcgcatctg gcgccgctgg acggacgcgc
     1261 agcggtccgc gtcgccgccg ccgtcctggc ggccgtcgcg acgaaacgag agagcctgtg
     1321 agatccggtt cttgcgtttc cgttgcgccc ggttcgtgga tcgatcgccg cccgctcgct
     1381 cgggtagcat tgccccgatg aacgcacccg atcgggcgac cccgtccgag gccgcagccg
     1441 cgatcgtctc gctcatctcc gacgccaagt tccgcgtcct cgccctgtcc gtgaccggcc
     1501 ttctgctgct gtggttcgtc gcagcggcgg tgtgggtcgc catgccggtc tggctgctcg
     1561 tcgccgcgtg gtggtaccgg cacgcgcgta ccgacgactg gctggtcctt gcgctggcgg
     1621 gcctgccctt ctggtgggcg acggggctcg cgcagtacgc gatccctgct ctggccggcc
     1681 ttggcgtgat cggcgcgatc cgggccgagc ctctgcgcgg ccggatcaag gacgcgtggg
     1741 agtcactcgg gctcgagaag tggctcgcgc tcggggcatt cctgtcgatg atcccgtccg
     1801 tcctggtcgt gagcgaaccc ggacggatct tcacctacat ccggaccgtc gggctgaccg
     1861 gcgtcgcgct cgcggggctg ctcgtggcca gcgcctggtc gcgaaagcac gacgcgcgcc
     1921 gcctgctgca cttcctggtc gggttcgccg gggtcgtggg tctgtggacg ctcgcacagg
     1981 tcgtctacaa gggctcgatc cacctgccgc tgcagcgaac cgcttccctg ctggaatggc
     2041 tgccgggcaa ggcgggcgag ctgacctcgc gcgcggttgg cgaggcggcc gggatcaagt
     2101 acgagaacgt cggcggagga cggctggtgg tgcggccgca cgcgttcttc atccacgcgg
     2161 tcgtcaccgg cgcgatgtcg gcgctgctcg gtccgctgat ctggcgccgc atccgtcacg
     2221 ttccggtctg gcgcgcggtg gcggccgcgt tcttcgccgg gctgaccggg atcatgctgg
     2281 tgtcctcgct cgcgcgcggc gcgtaccttg gcgtcgcgat cacggtgccg ctgttcgccg
     2341 tgatcttctg gccggacatc cgccgcgtcg cgccgaaggt gctcgccggc ggactcgcga
     2401 tcgcgatcgc ggtgagcggg atcgtgctgc tcacgcgcgg cggcgaggcc cgggcgcgcc
     2461 agtcgtcgca actcgccgaa ggatcgttcg tgtcgcgcgc cgcctactac cgcgcgacgc
     2521 tgcgcgcgat cccgcagcac ccgctcttcg gccacggcac ccagcgagat aacgtggccg
     2581 ccccgctgca ggtgcccgac ggcacgcgca ttctcgagat cccgaaaggc acgaccgtct
     2641 ccgtctacgg cgacggcagc ggaggccact acctgctgca cgacctgccc gcggagccgg
     2701 accggcgcat ccacgtccca tcggaccgaa cggttgccgc ccccgagacc ggtgacttcc
     2761 gggctccgga cggcgcgcac gccgacgtgc ttgccgacgg cggcgaggag gcaatcgtgt
     2821 cgaatgccga gcgagggatc atcgtggtcc gtaagttccc gtccggccgt caggagcgca
     2881 tctacttctc gaacgacgcc gcgcagcagg cgcggctcat ctccacgccc gaccgcgatc
     2941 cgccgctcgg ctcccactcg acgctgctcg gcgtgggcta caaatacggg atcctcggac
     3001 tgctggcatt cgtggcgctg tgggcgtaca cggggctcgc gccgctgcgg accctgcgag
     3061 gcggaacgcg gccgctgttc gacgacgcgc gggcgctgtg gttcggcatc accgccttcg
     3121 gcgcgcagtt cctgatctac gaatacgact tcgactcgtc ctccccggtg atcttctatc
     3181 tgctgtccgg gctgctgatc gggctgagcg cccgcccggc acgggtagag ttgtcgccgg
     3241 agcccgacag ccccgccgaa cccgcccgcg ccacctcccg atgaccacga ccgcccgaac
     3301 gcccgcgacc gatgcgccgt ccgcgaaagc gccggcgccg gccagcgtgt ggcggcgtcg
     3361 ctggctcgtc ctggttctgc tcgccgatct cgtcagctac gccatcgcgg tgttcggcgc
     3421 ctaccagatc cgcttcggtg ccggcgagcc cgcatacatg atcgtgaacc tgctcggcga
     3481 caaccgctac cggctcgcct acgtgccggc gttcgcgatc atgctgccgg tctggctcgg
     3541 cgtgctgttc ttcaaccgct gctactcctg gcggcacgtg tcctacccgc cattcgagct
     3601 gcgccgcgtg ctatcggcgc tcacgatggg cgtggcgatg ttcggcctgt tctcgttcgg
     3661 cttccgggcg aacatcaacc ggccgctgct gctcgtgctg ttcggggtcg ccacgatcac
     3721 cgtcccggcc gggcgtctgg tggcgcgcaa catctggaag cggtggcgcc gcacagcggg
     3781 cgcgcgcgcg cgcacgctga tcgtgggcgc caaccgcgaa ggacgcatcg tcgccgacat
     3841 catgatcgcc aatccgacct tcgacttcgt tccggtcgga ttcgtcgacg acgatcccga
     3901 gccgccctcg cccgacttcg tgcacgacat cccggtcgtc ggcggcacga aggagattct
     3961 ggatttggtg gactcgatga cgatctccgc ggtattcatc accgactcca cgctcggtcc
     4021 cgagcggacc ggggcgatct tgcgggcctt gcggccccgc gacgtggagg tgcgcatcag
     4081 cgcgaacctt cccgagatcc tcgcctggcg cctggccatc gagccgatca ccggtttccc
     4141 gatgctctcg gtcgacaacg gcaagatgga gcgcttcaac cgcttcgcca aacgaacgct
     4201 cgacgtcgcg ggatcggccg ccgggctgct cgtgctgtcg ccgctgctga tcgggctcgc
     4261 gatcgcgatc cgcgtcgact cgcccggccc ggtgttcttc cgccagcttc gcgccggcta
     4321 caagggaaag ccgttcaagg tgttcaagta ccgcaccatg gtcaacgacg ccgacaagat
     4381 caagctgtcc ggcgagatcg ccagcgaggt cggagggccg atcttcaagg ttcgcgacga
     4441 tccgcgcatc acgcgtgtgg gcaaactgct ccgccgatac tcgctcgacg agctgccgca
     4501 gctgatcaac gtgctgatcg gccagatgtc gctggtgggt ccgcggccac tgccgctgca
     4561 cgagcaggag gagctcgacc gcatcgcgcc ggagcggctc gaggtgcgtc cggggatcac
     4621 gggcctgtgg caggtcagcg ggcgctccga cgcgcccacc gagcggttgg tccgcctcga
     4681 cctgttctac gtcgagaact ggtccatcgc ctacgacctg tacgtgctct acaagacgat
     4741 tcccgcggtc ctgaagaagt ccggcgcgta ctgagagcgc gcaccaagac ggacgctcgc
     4801 ggttgctgta tcaaaagggt gaccgtatgt gttgccgtct tgacacagca accggagggc
     4861 gaaccgggga cggccgtcgg tcaggccgag cgggactcgc gccgggcgcg gcgcatctgc
     4921 cgcagcagct gcgggatgcc gcccagatag cgcttcgcga gccgccgcgg atcctgcgcg
     4981 aggcgccagg cccactcgag cgcggtcttt tgcacccagc gcggcgcgcg cgactcctcg
     5041 ccgacgatgt agccgaacgt gccgccgacg gtcatcagca gccgaacgcc ggtgtcgcgc
     5101 ccgtgacggt cggcccagat ctgctcgcgc ggacagccca tgccgaccag gatcagatgc
     5161 gcgccggatg cgcggatccg tgcggcgacc tccccgcctt ccgcatcggt gaagtagccg
     5221 tcgcggctgc cggctaccgt caatcccgga tatcgccgcg caagctcgtc cgctgcgcgc
     5281 tgcgcgagac cgggctcgcc gccgagaaga aagatcgagt gaccgtgcgc aacgcagtgc
     5341 tcgatcacct gatacataaa atcctgcgtt gcgatccgct cgggcagggt gtggccggcg
     5401 gcgcgcacgg tcatcagcac cgatgtcccg tcggcatgca ccaggtccgc gttggccagg
     5461 gcccgttcgt agtcgggatc gctaccgagg atgttcacgc cgacgacgtg gatcgcgtac
     5521 agcagcgtgg tgccctcgcg gtcgagcgcc tcgatcccgt agcgctccag ctgggcacgc
     5581 gtgacctggg cgatgtccac cccgagcacg cgaacgcgcg agatcccgtc cggataggtc
     5641 acgacgagga gtgctgcgct tcccgctcga gccgctttcg ctccgcgatc ttcggaccca
     5701 gcaggcggat catcaccagg tcggtgttcg cgaaggcctt ctcgtggaac acgctcgggt
     5761 ccgaggagtt cgcgtcgaag aagatcacga cctgatcctc gtcggactgc ccgaccgttc
     5821 cgatcgccgg atagccgaag tttgcggcct gacgtggccg ctggttggcg agacggctcg
     5881 tgaacagcgt gtagggcttg ccccagccgt tgcggttgcg gaagacgcgt gcgccgtcgg
     5941 cgatcgacac ctcgatcgtc atggtgcgac gggccgccca caccagcgcg atccttccgt
     6001 cggacagatt cgtcagccac gggctgatat cgctcggcgt atcgcccacg ttcatgaagc
     6061 ccatccgctt ccaggtgcgt ccgccgttcg ccgacacgta ttgatacagc ccgcccccgg
     6121 cctgcgggtt gggacccggc tcggtgcgcg agaccacgat caggcgcgcg ttgtcgttgg
     6181 tggtgccgtc caccaggatc gcgtcggcct cggtgggccg ctcgaacgat tggcggtcga
     6241 tcgtgacttc gttgccccag gtctcgccgt cgtcgtcgct gaagatcgcc cgcacgcgat
     6301 acgagtaccc gtcgttgccg taaagaagct gcatcagccg gccgcccggc agctcgacga
     6361 gcttgccgta cggcgaatac cagcggtcgg cgccgaccgg gaccgtcttg tagtcggacc
     6421 agctctgccc gtcgtcggtc gagcggatga agcccatgtc gtgccagatg cccgtgtgcg
     6481 agtcgtagcg ggcgaagaac gccacgatcg tgccggtcga ggtcacgcca ccggccgcgt
     6541 tgcggtcgtc ccactcgctg tcgtagatcg ggcgctcggt ccagccgcgt tcagcgccgc
     6601 gatactcgaa gaaaaccagc tgtccggtcg ggtcgatgct ggacgtgccg cgacgctgca
     6661 ccgcgaacaa tgtccctccg cgcgtcgcgc cgaccatacc gagcccctcg aacatcgccg
     6721 acacgtgcgt ggtcgcgcgt ccggagtcga acgcctgcgt gttgagcttc ttgcggaacg
     6781 tgccggtcgc cacacgcgcg agcggcccgg caccgcgtgc gggctcacgc atgctggcgc
     6841 gagcttgcgc ggtgacggtt cccgcaaagc cgacggtgcc ggtttgaccg caggtggccg
     6901 cggccacgac gagaacgagc agcaggggga gcagggcagg caaagcccgc cgggaagacc
     6961 tcatggcggc acgagtttat cccgtacgcg gaaggcggtc ccggaaggtt gtggataact
     7021 gggacagcac ggctagaatc gggcacggtc cacgcgaccg gcgggagggg aatccgatcg
     7081 ggacatcgca cggcaacaat gcaggccgga ttcggtcgcc ctttcgggcg acccaccgcg
     7141 gccgcgctcg cacccatttc tcttcccaat cgacggtttc cggagcaagt ttcactcctg
     7201 caagcaatca gttcccttca gggctcaagt taaaagaagg ttttgcgccg tgggtccgcg
     7261 aattgttgaa gcgtgacgat tcagacggtc acgctcggcc cgagtccgag cccgcgccca
     7321 tcggcgcgac cggccgtcgg gagcccgatc ctgcgggatc gcgaatccca tccaatatgg
     7381 cgaccagggc aagtccatat cccggtcatg gacgcatcgc ggtcgtagca aggcaaggga
     7441 agtaccggag gtgaccaggg tgacgaaaac gatgaaccgt gggcttcgcc gcaccaccgt
     7501 cggcacggtg gcgctcgcga tgctcgcgac gatgatgttc gtgcttgtcc atgaggaacc
     7561 ggcccgagcc gatcacatgg cggtgtcgga aggatgggac ttctcgccgg cccaagagcc
     7621 gttcacggcc cagaaggaca ctcccaagga gttcttcatc ttcttgaacc agtccgtgac
     7681 cacgcgaacc ctaatcatga tggagatcga gtcgggctcg tcggacccgg accagccgac
     7741 cgaagcgaac ggcttcacgc ccgacaactc gtattcgaag cccgaggcgt ggtgttacat
     7801 caacgcgggc gagtccacct gcacgatcac gtacacgttc acggtcgttc cgatcatcgg
     7861 cgtggacaac gtcatcgccc gcttctacat caacaagcac ctgatcgaca cgcaggagca
     7921 accgaacgag tcgacggcga accaatcctc gctcggctgt gacggggtgt tcggcggtcc
     7981 cgagcacgaa aacgagcccg acacgaccga ggcggctcag atcttcgtgt acggggcgat
     8041 cggtggcacc gctccgaatc cggtgcctcc gccgccgggc gtgcaggcgt cccgcacgtg
     8101 cgagaaccca ggcggcggcg gaaccccgac ccaatcaccg acccaatcac cgacgggcca
     8161 gccgacccaa tcaccgaccg gcgagccaac gcagtcgccc accggcgagc cgacccaatc
     8221 gccgacgcag tcacctaccg gccagccgac ctcgaacccg accaataccg gcccgagggg
     8281 gggccccggc ggaaccttcg gagagatgac gatgtcggtg tcgccgaacg tcggcaccta
     8341 cggaaccgac ttcctgatat ccggcgaggt cgtctgccac ggcgtgaagg tcgcgaatgc
     8401 ccgcctgcag atctaccgcc acttcgtggg cagccgctcg atcgacgtga tcgagacctc
     8461 gaccgacgtc cagggcaagt acgccttccg cgacacggac ctgcgcgcga gcgccgacta
     8521 ccagggaatg tggaacggcc ggacgggctc cacctcggcc tgcacctcgg gggccgagac
     8581 cggaccggac cggcgacgag ccgacgtgcg gccgggcgtg atcgtcaacg tccgggacac
     8641 cacgctgccc aagggcgagg cggccacgtt cttcggctcg gtcatcccgg cccatccggg
     8701 ccagaaggtc ttcctgcagg tctaccacgg ccagcaggga cgctgggaat acgcgaccga
     8761 gacccggctg gaccggcgct cgaactactc gatccgcttc agccgcaatg ccaccggcta
     8821 cctgctgttc cggatcgcat acccgaccca ggacctggat cactcctgga atatcagccg
     8881 aacggtccgc gtggattggt cctagcggcc actgagcaag ccagagcgag ccccgagggg
     8941 ctcgctctcg ttttcggggg caaccgcaag gcaggccggg tcgccgggta cggaccattg
     9001 aaagtgcgta gaatgccgct gacctcggtt caaggagttt tgagatgagc atgcctaacc
     9061 gtccgtcgtg gcgcccgctg gcgggagcga cgaccgtcct gctggtctta tcggcgatct
     9121 cgttcgtcgt gctgcgggac cagcccgccc gcgccgccca tagcgacgcc accatcgacg
     9181 tcgtcaacac ggccgagacc gtcaacggtc tgcccggcgg cacggtttcc tggagtgtga
     9241 ccgcgatgaa gacgaatgcg gatttctcac agagcccggt ggcgggcttg gtgatccttg
     9301 ccgagatcga gggtggcagc aatgaccccg atggcaatgt cgagggcgac caaacttttg
     9361 gattgagcta cggcactccg gacttgaagt gcacgacggg atccgacggg tcgtgcgaca
     9421 ttcagttcac gccctacgcc cttcagggga cggccgcgaa cgtgcgcatc tggatccagg
     9481 gcggggtcgg catcgacacc aacgaggggt tggtcgagac ctcgcaagcc ggctgtggga
     9541 ccgagcccga cggcaccgag gtggtcgcgt tcacgagcca gaccacgtcc ggaaccgccc
     9601 cggcccgcgc cgcccccgga gccgaaccga acccgaccgt gt
//
LOCUS       DATLFO010000021         8367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_99585, whole genome shotgun sequence.
ACCESSION   DATLFO010000021 DATLFO010000000
VERSION     DATLFO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99585"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VM841_00760"
     CDS             <1..555
                     /locus_tag="VM841_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL88742.1"
                     /translation="FADRVGAPIGSWSPLIPEQPVEIPLHDGERAGAGEIFLTAIHTP
                     GHASDHLCFWLEDERALFSGDHILGRGTTVVAHPDGDMVAYMRSLQIVKDRRASVIYP
                     GHGPVVTDPALVLNEYIAHRLMREQQVVDALPGTVDEIVAKIYHGVDPVLHPVAAMSV
                     RAHLAKLASEGIANEDAPDCWGPV"
     gene            552..4109
                     /locus_tag="VM841_00765"
     CDS             552..4109
                     /locus_tag="VM841_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL88743.1"
                     /translation="MIPCARCGTVVPDGARFCPSCGLSLSPAHREERRIVTILFADLV
                     GFTALSEASDPEVVKRIIDRAFEGLAETVTLYGGTVDKILGDEIMAVFGAPQAHEDDP
                     ERAVRCALEMQRALERYSRELSDERGLELAMRVGVNTGEVVAGAIGGGDAYTVVGDAV
                     NVASRLVKAAVPGSVLVGPSTRAATGSRIEYRPLDPLTVKGKAEPVADAEAVTERARP
                     RGGEGRAAPIVGRDAELSLLAAIADLVARDRRAAAVTIVGDAGMGKTRLVAEFTRRLS
                     DAQVLWGRSLPYGTASASHALEEIVREALGVSPGDDAPAARKRIAERAAALGLAAETD
                     RLAHLAGFREQPVLTGGRRAGGGAGPVAATPARSGEFADAAALFEVLVRSRRLLVLVF
                     QELHWAGAPVASAIGELLDRACDLPLLVVALGRLELAEITSGWPDRPGSTLLHLRPLA
                     RDAAADLLRGLAGARELTPAVRDSVLDRAGGNPFFIEEFVRLLTDRGQGDISVPVSVQ
                     GVVAARLDALDPGIKSILQDAAVTGEEFWPDALAAIEPHDPDTLRVALDVLVSRELIE
                     PVAGTHLAGRDEYRFRQTIVREVAYASLPKRARAAKHAAVAEWLEAVAGPDADSDHAD
                     LVAYHFERAALSAREVGLDDMPHAEKARAYLERAGDRAMGIDAATAAGGFYERALVFA
                     RDDEDALQLRVRLGEAYVGSWNPVAAETHLERALADARALGDRMVEGKALRLLGDLAR
                     IRGDLGLARPRLEQALQIARDTGNAHEEAEGLRSHGLLDMFDGRILAAPMWFRQALSR
                     ARELGDLRAVGWSLQNLGWANLLLGRVDESLSALDEGAEVFGGIGDLEGVGWCMGLRS
                     WGLLFRGQLDEADTLASQLIELAASTEQTSGGMGGMGVQVERVLRAIVAAYRLRFSDA
                     LTLASSTLEHFAAGESQWGLAMARYPIAVSHMLRLQIDDARAVLDEAAGHAADSGDPL
                     ARGLVDMIRAMTEIEAGELDEAERIARRGAAVTEQAGVGWHSLVLKRWIMAELARRRG
                     DVAGALAILEDPEPLPGSGLFPKGRLRAQHADVLCDAGRPKEAAAMARIAIEEAGPAV
                     VDVASARRILARALLESGEPAVALAAIEDAHAALEGREWDAERVRALALTARILDELG
                     RHDAAGEAYDRAGAILDAFGTDTDVRSLAALLGRGMPAG"
     gene            4166..4591
                     /locus_tag="VM841_00770"
     CDS             4166..4591
                     /locus_tag="VM841_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL88744.1"
                     /translation="MFGKIVVGTDGSDTASKAVQHAAGIAAATGSELLILSAYPPPKP
                     DAGSTFGGATPYAGPEIARSVLADAEKRLAGTAKVRTIMREGEAADAICDLAEEEGAG
                     LIVVGNRGMTGAKRFLLGSVPNNVSHHAPCSVLIVHTTD"
     gene            4614..4982
                     /locus_tag="VM841_00775"
     CDS             4614..4982
                     /locus_tag="VM841_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL88745.1"
                     /translation="MYTKIMSATDGSATAAKAVAIAADLARAVDAELLLVHVGDPDKG
                     RTVLEEAVSKFARGLKTKTMTVSGDPADMIIESAEAEGADLLVVGNKGMTGAKRFLLG
                     SVPNQVSHHSPCNVLIVKTT"
     gene            complement(5043..5426)
                     /locus_tag="VM841_00780"
     CDS             complement(5043..5426)
                     /locus_tag="VM841_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL88746.1"
                     /translation="MPARILVVDDDPAVREYASTTLESAGFAVTDVASGEQALDAMRE
                     QPFDVIVLDKAMPGMDGDEVARRLRDEPGGGQLAIVMLSGLDSDQDQWDGWRAGVDIY
                     VTKPFDDDELIAHIRRLLRLRAMAR"
     gene            5612..6304
                     /locus_tag="VM841_00785"
     CDS             5612..6304
                     /locus_tag="VM841_00785"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HVL88747.1"
                     /translation="MLEEVLANPEAVASRVATLVAEEAPATVAIASSPSLRPAIVAAA
                     GALSNAAIWFADDRCGPTSHPESAAGFAAGALPGAGLHRIRGEDPPGEAARIYDEEIR
                     AVLGEEPVFDLVLLAAGGDGRVAGLFPDAPELGERERRAVATGDAHGGLRRVTLTLPV
                     LNRARRALICATGSDKAVAVARCRDGELLPAARVLGATWLLDEAAAAPPPRPARRKPW
                     DPLEGQEVLFEL"
     gene            complement(6314..7099)
                     /locus_tag="VM841_00790"
     CDS             complement(6314..7099)
                     /locus_tag="VM841_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88748.1"
                     /translation="MRGASALVALTLSLAACSGGETPAPEATGSPARPPTVTMQVFGL
                     DLAKDEAAVLVIDPAIPAAGVIVSHDPAARVDVFLLKSATDAPGECPIEVGPRTNRTC
                     VASIGTGVRESLEQRGEAGGVAVVLRDGPGRMDVRLDYDERSRRVGLRLPRLAPPAGA
                     SVCKDNGCNPFIEVTPLRSGPMTATAAFSGGPGRLQVQSGRVIAKAFTASGQPYRIPD
                     EDLGDAPLKVSTRFDVPAEYALALINENRGAALNDIVIDATWP"
     gene            complement(7137..7925)
                     /locus_tag="VM841_00795"
     CDS             complement(7137..7925)
                     /locus_tag="VM841_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL88749.1"
                     /translation="MPLIDGPDCRLNVEVRGEGAPVTIFAHGITSCIAEIEPYAAATP
                     GTRVMMDLRGHGHSDRPPPEAGYGPDAMRRDIAWVADRFGATRAFGVSVSADILSAIL
                     ADDPDRFERIVLFLPGALDERNEGAAHFPDFAADLEAMTLEDYATKALADPVYGLLFA
                     KRPTWRALVRQRILRMNRDGVPRALRAYRDAPPPVPEPERLRAVSGPALILAHEDDPV
                     HDAAVARRLAAFLPNATLRVWDEPLAMLDDMPAFARLVAGFLGA"
     gene            7950..>8367
                     /locus_tag="VM841_00800"
     CDS             7950..>8367
                     /locus_tag="VM841_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVL88750.1"
                     /translation="MSVIVGTSGWQYDDWRDELYGTTPKTRWLEAFAQRLRAVEVNNT
                     FYRLPSAETFASWRDRTPDGFVFAIKASRFITHVRRLEDVSEPVTVLIERASALARKL
                     GPVLYQLPPSLRRDDALLARFLDALPPYPKAAIEFRH"
BASE COUNT         1163 a         2907 c         3087 g         1210 t
ORIGIN      
        1 ttcgccgacc gtgtcggcgc gccgatcggg tcctggtcgc cgctgatccc ggagcagccg
       61 gtcgagatcc cgctgcatga cggcgagcgt gccggagccg gcgagatttt cctgaccgcg
      121 atccacacgc ccggacacgc cagcgatcac ctgtgcttct ggctcgaaga cgaacgcgcg
      181 ctgttttcgg gcgatcacat cctgggccgc ggcacgacgg tcgtggcgca tcccgacggc
      241 gacatggtcg cctatatgcg ttcgcttcaa atcgtgaagg accgccgcgc gtcggtgatt
      301 tatcccggcc acggcccggt tgtgaccgat cccgcgctcg tcctcaatga gtacatcgcg
      361 catcgtttga tgcgcgagca gcaggtcgtg gatgcgctgc cgggcaccgt cgacgagatc
      421 gtcgcgaaga tctatcacgg cgtggatccg gtgcttcacc cggtcgcggc catgagcgtg
      481 cgggcgcacc tggccaagct cgcgtccgag ggcatcgcga acgaggacgc gccggactgc
      541 tggggacccg tgtgatcccc tgcgcgcgct gcggaaccgt cgttccggac ggcgcgcgct
      601 tttgcccgtc gtgcgggctg agcctttctc cggcccaccg cgaagagcgg cgcatcgtca
      661 cgatcttgtt cgccgatctc gtgggcttca ccgcattgag cgaggcgagc gatcccgagg
      721 tggtcaagcg gatcatcgac cgcgccttcg agggtctcgc cgagacggtg accctctacg
      781 gcggaaccgt ggacaagatt ttgggtgacg agatcatggc cgtgttcggc gcgccgcagg
      841 cgcacgagga cgatcccgag cgcgccgtgc gctgcgcgct cgagatgcag cgggcgctcg
      901 agcgctactc gcgtgagctg tccgacgagc gtggcctcga actcgcgatg cgtgtcggag
      961 tcaacaccgg cgaggtcgtc gcgggtgcga tcggcggcgg cgacgcctac acggtggtcg
     1021 gggatgcggt gaacgtcgcc tcgcggctgg tcaaggcggc cgtgccggga agcgttctcg
     1081 tgggcccgtc cacgcgcgcc gccaccggtt cacgcatcga gtaccggccg ctggatccgt
     1141 tgaccgtgaa aggcaaggcc gagccggtcg ccgacgccga ggccgttacc gagcgggcgc
     1201 gtccacgcgg cggcgagggc cgggccgcac cgatcgtcgg ccgcgacgcg gagctttcgc
     1261 ttctcgctgc gatcgccgac ctcgtggcgc gggatcgccg ggccgccgcg gtgaccatcg
     1321 tcggcgacgc cggcatgggc aagacgcggc tggtggccga gttcacccgc cgcctgtccg
     1381 acgcgcaggt cctgtggggc cggtccctgc cctacgggac cgcctcggcg tcgcacgcgc
     1441 tcgaagagat cgtgcgcgag gcgctcggcg tctcgcccgg cgacgacgcg ccggcggcgc
     1501 gcaagcgcat cgccgagcgt gccgcggcgc tggggctcgc ggccgagacc gacaggctcg
     1561 cgcatctggc cggctttcgc gagcagccgg tgctgaccgg cgggcggcgc gccggcggtg
     1621 gtgcgggccc cgtcgccgcg acgccggcgc gcagcggcga gttcgccgat gccgccgcgc
     1681 tgttcgaggt gctcgtgcgc agccggcggc tgctcgtgct cgtgttccag gagctgcact
     1741 gggccggcgc gcccgtcgcg agcgccatcg gggagctgct cgaccgcgcc tgcgatctgc
     1801 cgctgctcgt cgttgcgctc ggccgtcttg agctcgccga gatcaccagc ggctggcccg
     1861 accggcccgg ctcaacgctg ctgcatctgc gcccgctcgc gcgcgacgcc gccgcggacc
     1921 tgctgcgcgg cctggccggc gcacgcgagc tgacacccgc cgtgcgcgac agcgtgctcg
     1981 atcgcgccgg ggggaatccg ttcttcatcg aggagttcgt gcgcctgctg accgaccgcg
     2041 ggcagggcga catcagcgtg ccggtcagtg tgcagggcgt cgtcgcggcg cggctcgacg
     2101 cgctcgatcc cggcatcaag tcgatcctgc aggacgcggc ggtgaccggg gaggagttct
     2161 ggccggacgc gctcgccgcg atcgagccgc acgatcccga cacgctgcgc gtcgcgctcg
     2221 acgtgctcgt gtcgcgcgag ctcatcgagc ccgtcgccgg gacacacctg gccggccggg
     2281 acgagtaccg cttccggcag acgatcgtgc gcgaggtcgc gtacgcctcg ctgcccaagc
     2341 gcgcgcgcgc cgccaagcac gcggcggtcg ccgagtggct cgaagccgtg gcggggccgg
     2401 acgccgattc cgatcatgcg gatctggtcg cctatcactt cgagcgcgcc gcgctcagcg
     2461 cacgcgaggt cggcctcgac gacatgccgc acgccgaaaa ggcacgcgcc tacctagagc
     2521 gcgcgggtga ccgggcgatg ggcatcgacg cggcaaccgc cgccggaggc ttctacgagc
     2581 gcgccctcgt cttcgcgcgc gacgacgagg acgcgctgca gctgcgcgtg cgcctcggtg
     2641 aggcgtacgt cggctcgtgg aatcccgtcg ccgccgagac gcacctggag cgcgcgcttg
     2701 ccgatgcgcg tgcgctcggc gaccggatgg tggagggaaa ggcgctcagg ctgctgggag
     2761 atctcgcccg gatccgcggc gatctcgggc tcgcgcgccc gcggctggag caggcgctgc
     2821 aaatcgcgcg cgacaccggc aacgcgcacg aagaggcgga gggcctgcgc tcccacggcc
     2881 tgctcgacat gttcgacgga cgtatcctgg cggcgccgat gtggttccgg caggcgctgt
     2941 cccgcgcgcg cgagctcggc gacctgcgcg cggtcggatg gagcttgcag aacctgggct
     3001 gggcgaacct gctgctggga cgggtggacg aatcgctgtc ggcgctcgac gaaggcgccg
     3061 aggtgttcgg cggcatcggc gacctcgaag gcgtcggctg gtgcatgggt cttcgaagct
     3121 ggggcctgct gttccgcggc cagctcgacg aagccgacac gctcgcgtcg cagctcatcg
     3181 agctcgccgc aagcaccgag cagacgagcg gcggcatggg cggcatgggc gtgcaggtcg
     3241 agcgcgtcct gcgtgcgatc gtggcggcat atcgcctgcg gttctccgac gcgctcacgc
     3301 tggcaagctc gacgcttgag catttcgccg ccggcgagtc gcaatggggg ctcgccatgg
     3361 cgcggtatcc gatcgcggtt tcccacatgc tccggctgca gatcgacgac gcgcgcgcgg
     3421 tgctggacga ggccgccggt cacgcagccg attccggtga cccgctcgcc cggggactgg
     3481 tggacatgat tcgtgcgatg accgagatcg aggcaggcga gctcgacgaa gccgagcgca
     3541 tcgcccggcg cggtgcggcg gtgaccgagc aggccggtgt gggctggcac agcctggttc
     3601 tcaagcgctg gatcatggcg gagctggcgc gccgccgcgg cgacgtcgcg ggtgcgctcg
     3661 cgatcctgga ggatcccgag ccgctgccgg gatccggtct gttccccaag ggccggctgc
     3721 gcgcccagca tgcggacgtg ctctgcgacg cggggcggcc gaaggaggcg gcggcgatgg
     3781 cgagaatcgc cattgaggaa gcgggccccg ccgtggtcga cgtcgcgtcc gcccgccgga
     3841 tcttggcgcg ggcgctgctc gagagcggcg agcccgcggt cgcactcgcg gcgatcgaag
     3901 acgcacacgc ggcgctcgaa ggccgggagt gggatgccga gcgggtgcgg gcgctcgcgc
     3961 tgaccgcgcg gatcctcgac gagctcggcc ggcatgacgc ggcgggggag gcctacgacc
     4021 gcgccggagc catcctggac gcgttcggga ccgacaccga tgtgcggtcg ctggccgcgc
     4081 tgctcgggcg cgggatgccg gcaggctaga tttgccgggc tgcgcggcgg tagggttatc
     4141 gcgttccgac tgaatagggg gagcgatgtt cgggaagatc gtggtcggca ccgacggttc
     4201 ggacacggca tccaaagccg tgcagcacgc ggccggcatc gccgcggcga ccggctccga
     4261 gttgctgatc ctgtcggcgt atccaccacc gaagccggac gccggctcga cgttcggcgg
     4321 tgcgactccc tacgcgggac ccgagatcgc gcgcagcgtc cttgccgacg ccgagaagcg
     4381 tctggccggc acggcaaagg tccgtacgat catgcgcgaa ggcgaagcgg ccgacgcgat
     4441 ctgcgatctc gccgaggaag aaggggcggg cttgatcgtc gtggggaacc gcggcatgac
     4501 gggcgccaag cgcttcctgc tcggcagcgt gcccaacaac gtctcgcacc acgcaccctg
     4561 ctcggtgctg atcgtgcaca cgaccgactg acgtcgcggc catcggagga cgcatgtaca
     4621 ccaagatcat gagcgcgacc gacggctcgg cgaccgcggc gaaggccgtg gccatcgcgg
     4681 cggatctcgc gcgggcggtc gacgcggagc tgctgttggt ccacgtcggt gatcccgaca
     4741 agggacgcac ggtgctcgag gaagccgtca gcaaattcgc ccggggcctc aagacgaaga
     4801 cgatgacggt gtcgggcgac ccggcggaca tgatcatcga atcggccgag gccgaaggcg
     4861 ccgacctgct cgtggtgggt aacaagggca tgacgggcgc gaagcgtttc ctgctcggca
     4921 gcgtgccgaa tcaggtctcg catcactcgc cctgcaacgt gctcatcgtt aagacgactt
     4981 gacccgaagg agcgcggagc ggccggccat tggccggccg ttgctcgtgc gaggcgccgt
     5041 cgctagcgcg ccatcgcgcg taagcgcagc aaacggcgga tgtgggcgat gagctcgtcg
     5101 tcgtcgaacg gcttcgtgac gtagatgtcc acgcccgcgc gccagccgtc ccactggtcc
     5161 tgatccgagt ccaggcccga cagcatcacg atcgcgagct ggccgccgcc gggctcgtca
     5221 cgcaggcgcc gcgcgacctc gtcgccgtcc atgccgggca tggccttgtc gagcacgatc
     5281 acgtcgaaag gctgttcgcg catcgcgtcg agcgcttgtt ctccggacgc gacgtcggtg
     5341 accgcgaacc cggcgctttc gagcgtcgtc gatgcatact cgcgaaccgc cggatcgtcg
     5401 tccaccacta ggattcgtgc aggcattcaa ccttcctccg cccttgctat cggagccaaa
     5461 ggtcctgcgc ttgaccctca gagcagacta aacgcgcccc atcccatcga agcagacacc
     5521 ctcccgccgc atgccccggg gacgccaggg gacctccatt tccggcgaat gtcgtgctcg
     5581 ggtgcatttc ggcacgcggt aggctgcggt catgctggag gaggtcctgg caaacccgga
     5641 ggccgtggcc tcgcgcgtcg cgaccctggt cgccgaggag gccccggcca cagtggcgat
     5701 cgcctcgtcc ccgtcgcttc ggccggccat cgtcgccgcg gccggggcgt tgtcgaatgc
     5761 cgcgatctgg ttcgccgacg accggtgcgg tcccacctcg cacccggagt ccgcggcggg
     5821 tttcgccgcc ggtgcgctcc cgggcgccgg cctgcatcgg atccgcggtg aggacccgcc
     5881 cggcgaggcc gcgcgcatct acgacgagga gatccgcgcg gtgctgggcg aggagccggt
     5941 gttcgatctg gtgctgctcg ccgcgggcgg cgacgggaga gtcgcgggcc tgtttcccga
     6001 cgcgcccgag ctgggggagc gcgaacgtcg cgcggttgcg accggcgacg cccacggcgg
     6061 cctgcggcgc gtcacgctca cgctgccggt gctcaaccgg gcgcgaaggg cgttgatctg
     6121 cgcgaccggc tcggacaagg ccgtcgcggt cgcgcgctgc cgggacggcg agcttctgcc
     6181 ggcggcgcgt gtgctgggcg cgacgtggct gctcgacgag gcagccgccg ccccgccgcc
     6241 gcggccggcc cggcgcaagc cgtgggatcc gctcgagggc caggaggtcc tgttcgagct
     6301 gtagcgcgcg ggcctatggc caggtggcgt cgatgacgat gtcgttgagc gccgcgcccc
     6361 ggttctcgtt gatgagcgcc agcgcatact cggccggcac gtcgaagcgg gtcgagacct
     6421 tcagcggcgc gtcgccgaga tcctcgtcgg ggatccggta gggctgcccg ctcgcggtga
     6481 acgccttggc gatcacgcgt ccgctctgca cctgaagccg tcccggacca ccggagaagg
     6541 ccgccgtggc cgtcatcggc ccgctgcgca gcggtgtcac ttcgatgaac gggttacagc
     6601 cgttgtcctt gcacaccgac gcgcccgcgg gcggggccag gcgcggcaac cgcagcccga
     6661 cgcggcggct gcgctcgtcg tagtccagac gcacgtccat gcgtccggga ccgtcgcgca
     6721 gcacgacggc gacgccgccc gcctcgccgc gctgctcgag cgattcccgc acgccggtcc
     6781 cgatcgaggc gacacaggtc cggttcgtgc gcgggccgac ctcgatgggg cattcaccgg
     6841 gcgcgtcggt agccgacttc agcaggaaca cgtccacgcg cgcggccgga tcgtgcgaca
     6901 cgatcacgcc cgccgctggg atggccggat cgatcacgag caccgccgcc tcgtccttgg
     6961 ccaggtccag gccgaacacc tgcatcgtca cggtcggcgg cctggccggc gagcccgttg
     7021 cctcgggcgc gggggtctcg ccgcccgagc aggccgcgag cgaaagggtc agagcaacaa
     7081 gagcggatgc gccacgcatg acgggccaca cgatacccag gacgcggccg ggccgctcac
     7141 gcgccgagga agccggcgac gagccgtgcg aacgcgggca tgtcgtcgag catcgcgagg
     7201 ggctcgtccc acacgcgaag cgtggcgttc gggagaaagg cggcgaggcg ccgcgcgacc
     7261 gccgcgtcgt gcacgggatc gtcctcgtgc gccaggatca gcgcgggccc ggacacggcg
     7321 cgcagacgct ccggctcggg caccggcgga ggcgcgtcgc ggtatgcgcg aagcgcgcgg
     7381 ggcacgccgt cccggttcat gcgcaggatc cgctgccgca cgagcgcgcg ccacgtcggc
     7441 cgtttggcga acagcagccc gtagaccggg tcggcgagcg ccttcgtcgc gtagtcctcg
     7501 agcgtcatcg cttcgaggtc ggcggcgaag tcgggaaagt gcgctgctcc ttcgttgcgc
     7561 tcgtcgagtg cgccgggcag gaacagcacg atccgctcga agcggtccgg atcgtcggcc
     7621 aggatcgcgc tcaggatgtc ggccgagacc gacacgccga acgcgcgggt ggcgccgaac
     7681 cggtccgcca cccacgcgat gtcgcgccgc atcgcgtccg ggccgtagcc ggcctcgggc
     7741 ggcggccggt cgctgtggcc gtgcccgcgc aggtccatca tcacgcgcgt gccgggcgtc
     7801 gcggctgcgt agggctcgat ctcggcgatg caggaggtga tgccgtgcgc gaagatcgtc
     7861 acgggcgcgc cttcgccgcg cacctcgacg ttgaggcggc agtcggggcc gtcgatcagc
     7921 ggcatgaggc ggcgtagagt accagcggta tgagcgtcat cgtcgggacc agcggctggc
     7981 agtacgacga ctggcgcgac gagctctacg gcacgacccc gaagacccgc tggctcgagg
     8041 cgttcgcgca acgtctgcgc gccgtcgagg tcaacaacac cttctaccgg cttccgtccg
     8101 ccgagacgtt cgcgtcgtgg cgggaccgga cccccgacgg gttcgtcttc gcgatcaagg
     8161 cttcgcgctt catcacgcac gtgcgcaggc tcgaggatgt ctcggaaccg gtgacggtgc
     8221 tgatcgagcg ggccagcgcg ctcgcccgaa agctcgggcc ggttttgtac cagttgccgc
     8281 cgtcgctgcg ccgcgacgac gcgctgctcg cacgatttct cgacgcgctt ccgccgtatc
     8341 ccaaggcggc gatcgagttc cgccacg
//
LOCUS       DATLFO010000022        10633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590 k141_470,
            whole genome shotgun sequence.
ACCESSION   DATLFO010000022 DATLFO010000000
VERSION     DATLFO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VM841_00805"
     CDS             <1..398
                     /locus_tag="VM841_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL88751.1"
                     /translation="CMKRSILVVENDPMSRRLAERVLTAGGYDVACVGDAETALESAR
                     AHRPDLVLMDVRLPGMDGLEATRRLLADPATADVAVAALSAQAFDEDVRSALDAGCVD
                     YLTKPIGARELLERVARILDGDGAEKAGR"
     gene            395..1426
                     /locus_tag="VM841_00810"
     CDS             395..1426
                     /locus_tag="VM841_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVL88752.1"
                     /translation="MSLVPAGLAPGHVRVVVTDDQPHMRLMLTHALSIGEEFEVVGQA
                     ADGAEAVELVESMQPDVLLIDLAMPNMDGFEAIKRIRRRGSRVRILVLTSNGDRASIA
                     QAMENGADGYVLKGAGVEEIRVALRDVAAGGAVLAPAIAREVVDGFIGLVEEKRRRDV
                     AVIRTLAAAVEARDRVTGDHINNVANLSVALWTHMVGSKPDEEMVYGFLLHDVGKISI
                     PDSILLKPGKLDDDEMARMRRHVEVGLDLIEPLGFGRVVTDVIRCHHERFDGEGYPNG
                     LRGEQIPIHARMFSVVDAYDAMTADRPYRKGMPPADALAELRRFAGTQFDPDCVDGFE
                     ALAVELGFA"
     gene            1649..2842
                     /gene="wecB"
                     /locus_tag="VM841_00815"
     CDS             1649..2842
                     /gene="wecB"
                     /locus_tag="VM841_00815"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HVL88753.1"
                     /translation="MAEPMHGNVKPLPTWGRGQERVLFVFGTRPEAIKLAPVIKACAA
                     EPRLEPYTVVVRQHHEILDQTLEVFGLEPDHVVEMVRNGHGLVELTAKVLEGVGAAIV
                     ESAPSLVVVQGDTTTAYASALAAFYSGVPVAHVEAGLRTNNLAHPYPEELNRQMVARI
                     ADLHFAPTRAAYKNLMREGVPSERVVITGNTVVDALHSIMPQDAASRRRGEVVRVLAT
                     AHRRENWGGPLGEICRALRDIAEAHANVEILFVVHPNPIVRRAAEEALGGLENIRISP
                     PLAYRDFVAHMASANLILTDSGGIQEEAPTFGTPVVILRETTERVEGIESGSAIIAGT
                     TRDSIRAAAFAALARGEGKPKTRTLYGDGKASERILAALMDRFFGSAEEEIEETGSAL
                     DGVRA"
     gene            2915..3700
                     /locus_tag="VM841_00820"
     CDS             2915..3700
                     /locus_tag="VM841_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88754.1"
                     /translation="MNISAGLRPVRQPSHRALHRFPFDKRATVAGLAVATALVAASLL
                     FANALWIFEADALGALLRMSDTPFSVAGEPARLLGGAVAGGARFTVPGTGSPIPAGAA
                     LGGIAAFVAASIVAIRWRRVPVPAKVLWLLFAAIGSATLWYTGFVSGSPPQPVRSLSI
                     GFQRGGLLTVVVAALLFAFEIFPVPGRLRTKAGWLLGLCLFAVAWSVVRMAVVLATAH
                     HIGPWTFLYLHYLAGPVVDFLTIVAFYSFATYGLSKRLEEALA"
     gene            3697..4983
                     /locus_tag="VM841_00825"
     CDS             3697..4983
                     /locus_tag="VM841_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL88755.1"
                     /translation="MTIAVQVLSFAIFVVVTAYAVRHYRFLWNRMFERQDRAYQDLAG
                     SYLPSVTVIIPMHNESRVAAGVIEHLVAADYPQELLQILAVNDHSSDDTAEIINAVAA
                     RYPNVTPVHRTDPGEARGKTEALKTGTAMATGEVILVFDADYRPGRGLLKRLVAPFCD
                     PGVGAVMGRVVPSNSAANALTRLLDLERAGGYQANLQARQNLGLLVQYGGTVGGVRRS
                     VLEAVGGWVPHLTEDTDLTLRIYLRGFRVVYVNIAECYEEVVQDWAARTKQLSRWAVG
                     HNMVMLDYLGPTLRSKVLTPRQKIEGVLLLGVYLAPVMLLAGWIASIYLAFVSSGWFL
                     TVYTGILGSLSFNAWGNLAAFNEIGSALLLDRRTRSILLLPLAIFSSFAGMWICTGAL
                     MKTVKARIRKTGLAWDKTPRYTQDAAPAPAEPETSR"
     gene            4980..6131
                     /locus_tag="VM841_00830"
     CDS             4980..6131
                     /locus_tag="VM841_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88756.1"
                     /translation="MSLGYVALVGIFFAMIASLFAMALAEIRRPRDPGPLPIDVGRDI
                     EHRWFARVLRGLPMGDVATAGDAGAADAPLLIFAAPTGEGTYMMRGDGDVPGGGVLDA
                     FVCDGIATVGPGAAVLEHVDGEAGLVVGAGASVGGRATSATWILLAPGSHVRYAAAPQ
                     IMTDGPTVPEDPAALAVWDGSMDVDLEWLITQGMPVDQALRSLSRDQGRPLDGIAAVS
                     ALEARTGIGAATLDLGAAINPWWLRDAEGWYLGAATARIPGDLALPAGAVVPFHLIVD
                     GTLVAREGVHFHGGVSVRGDAVLFDGCRVERSLTAKGNVLLSDRCSVGECVSAGGDAT
                     LGASVLIGGVRNGGLASTGSVALGPGVRVTNKIYADGGIVVLAPDAAAL"
     gene            complement(6141..6791)
                     /locus_tag="VM841_00835"
     CDS             complement(6141..6791)
                     /locus_tag="VM841_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL88757.1"
                     /translation="METPHPPATPEFGSVTRNAAPHVYPSGASFEVRKITVGAYDNNV
                     YALRSGGQVLIVDGADEPDRIAAMAGDDEVVGIVQTHGHFDHVQALASLVERFRCPVR
                     AHPADPMPVTTVPLSHGETIRVGETDVTVLHTPGHTPGSVCFLAGSFLFSGDTLFPGG
                     PGNTRGNPAAFAQIMQQLDEHLFTLPDDTRVCPGHGLDTTIGRERPHVEVWRERGW"
     gene            6911..9760
                     /gene="uvrA"
                     /locus_tag="VM841_00840"
     CDS             6911..9760
                     /gene="uvrA"
                     /locus_tag="VM841_00840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HVL88758.1"
                     /translation="MSSDKIVVHGAREHNLKDIHLEFPRDAMVVFTGISGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDFIEGLSPAISIDQKTAGHNPRSTVGT
                     VTEIYDYLRLLYARIGVPHCPTCGREVARQTPDQIVDQIHALPEGTRFQVLAPVVRDR
                     KGEYATLVEDLSKKGFARAKIDGEVRELSDKIRLDRYYKHTIEVIVDRLVMKPGIERR
                     VADSVETALNLAEGLVSIELVDHGDVQVYSQHLACMHCGLSFDELQPKNFSFNSPYGA
                     CERCDGLGTHLEVDPELIVPDEDLSIEEGALAPWASGRTEYFARMLESVAAYAKVSIK
                     KPWKSLTKKQKDIFLYGTGDDTIHVKYRNRYGRSRSYDTYYEGVIGWLQRKHDEVDSD
                     YHREKLEEYMREIPCPACKGSRLKPESLSVAIGGKNIAEVTSMSIAEADKFLGTLEWD
                     EREHMIGDRIIKEIAARLGFLLDVGLDYLTLGRSANTLAGGEAQRIRLATQIGSGLVG
                     VLYILDEPSVGLHQRDNHRLIETLVRLRDIGNTLVVVEHDEATIATADYVVDIGPGAG
                     EHGGSLVYAGDYKGLLKSKESLTGQYLSGRRSIPVPGMRRPPGGNWLGLKGARQNNLK
                     NIDVKIPLACFVAITGVSGSGKSSLLTDTLYPALMRHVYASRTVPGKHKALTGIHHID
                     KVVNVDQAPIGRTPRSNPATYIGVFDHVRSLFSKTPEARSRGYQPGRFSFNVKGGRCE
                     ACTGDGQIKIEMHFLPDIYVPCEICKGKRYNRETLDVTFKGKNISQILEMSVEEALSV
                     FEAVPVIHRHLQTLFDVGLGYVKLGQPATTLSGGEAQRVKLASELSKRATGRTLYILD
                     EPTTGLHFEDINKLLGVLQRLVDQGNTVVVIEHNLDVIKTADWIIDLGPEGGSGGGEI
                     VAEGTPEDVAKEPGSYTGRFLREALGLPADDAATG"
     gene            complement(9757..10530)
                     /locus_tag="VM841_00845"
     CDS             complement(9757..10530)
                     /locus_tag="VM841_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88759.1"
                     /translation="MGLRVWMAGLCSVWLLAAGPARAEARDAAVAVQVPVPHGPVSVL
                     VRCTISPAAASGAWIAAFAVDADSTGYVESSVTALGWWDHGRLDLVVRRDGIRIEAPP
                     VEVVTVGERYRITMTATWGGGHRLWASTLRGGCGATEGTVTEISAILNGADVTASGVD
                     ARVLTAGARQFPRSVAAQGATLVHGSFAASSPYLLGVFSARSGALSVTAPDGSVRSDA
                     GLYPSIPVRNGTGGTWRFDADTAVSDDPYLLTALAFAKR"
BASE COUNT         1672 a         3700 c         3623 g         1638 t
ORIGIN      
        1 gttgcatgaa gcgttccatc ctcgtcgtcg aaaacgatcc gatgtcgcgc cggctcgccg
       61 agcgcgtgct cactgcaggc gggtatgacg tcgcgtgcgt cggagacgcc gagacggcgc
      121 tcgagtcggc gcgcgcgcac cgtcccgacc tcgtcctgat ggacgtccgg cttcccggga
      181 tggatgggct cgaggcgacg cgccggctgc tcgccgatcc ggcaaccgcc gacgtcgcgg
      241 tggccgcgct cagcgctcaa gcattcgacg aggacgtgcg cagcgcgctc gacgcgggtt
      301 gcgtggatta tctcaccaag ccgatcgggg cgcgcgagct gctcgagcgg gtcgcgcgca
      361 tcctcgacgg cgacggtgcc gaaaaggccg gccgatgagc ctcgtccccg cgggcctggc
      421 gcccgggcac gttcgcgtcg tcgtcaccga cgatcagccg cacatgcggc tcatgctcac
      481 ccatgccctg tccatcggcg aggagttcga ggtcgtcgga caggcggccg acggtgcgga
      541 ggcggtggaa ctcgtcgagt cgatgcagcc cgacgtgctg ctgatcgatc tcgcgatgcc
      601 gaacatggac ggcttcgagg cgatcaagcg gattcgccgc cgcgggagcc gcgtccgcat
      661 cctggtgctc acctcgaacg gggaccgcgc ctcgatcgcg caggccatgg agaacggcgc
      721 cgacggctac gtgctcaagg gcgccggcgt ggaggagatt cgcgtcgcgc tgcgcgatgt
      781 ggcggccggc ggggccgtgc tcgccccggc gatcgcgcgt gaggtcgtgg atgggttcat
      841 cggcctggtc gaggagaagc gccgccgcga cgtggccgtc atccgtacgc tcgcggccgc
      901 cgtcgaggct cgcgaccgcg tcaccggcga ccacatcaac aacgtcgcga acttgtcggt
      961 cgcgctctgg acgcatatgg tcggctcgaa gccggacgag gagatggtct acggctttct
     1021 gctccacgac gtcggcaaga tctcgattcc cgactccatt ctgctcaagc cgggaaagct
     1081 cgacgatgac gagatggcgc gcatgcgccg gcacgtcgag gtcgggctcg atctgatcga
     1141 gcccctcggc ttcggccgcg tcgtgaccga cgtgattcga tgccatcacg agcgtttcga
     1201 cggcgagggc tatccgaacg gccttcgcgg tgagcagatc ccgatccacg cgcgcatgtt
     1261 cagcgtcgtc gacgcctacg acgcgatgac cgcggaccgg ccgtatcgaa aaggaatgcc
     1321 gccggccgac gcgctcgccg agcttcgtcg cttcgcaggc acgcagttcg atcccgattg
     1381 cgtggacggc ttcgaagcgc tcgcggtcga gctcgggttc gcgtagcgac ttcgcgtcgt
     1441 actccccaac ggtttcgttt cttccgattc acccaccgaa ccggcaacaa cgaaactcgc
     1501 gcacgcgaac aagttccaat tcgcacgcgc gtgccgccgc ctcgcgggag ggaatgcgag
     1561 acgacgcggc gaacaacact gccatcgccg gggacgacat cgcctgccac cggggggcgg
     1621 gacgcggtcc cgggaagggg agtcgtcgat ggcagagccg atgcacggca acgtgaaacc
     1681 gctgcccacg tggggacgtg gccaagagcg cgttctgttc gtgttcggca ctcgtcccga
     1741 agccatcaag ctcgcccccg tcatcaaagc ctgcgccgcc gagccgcgtt tggagccgta
     1801 cacggtcgtc gtgcgccagc accacgagat tctcgaccag accctcgagg tgttcggcct
     1861 cgagccggat cacgtggtcg agatggttcg caacggacac ggtctcgtcg aactgaccgc
     1921 gaaggttctc gaaggtgtcg gcgctgcgat cgtcgagtcg gcgccgtcgc tggtcgtcgt
     1981 tcagggggac accacgaccg cgtacgcatc cgctctggcg gccttctact cgggcgttcc
     2041 cgtcgcgcac gtcgaagccg ggctgcgcac caacaacctc gcacatccgt atccggaaga
     2101 gctcaaccgg cagatggtcg cgcgcatcgc cgatctgcac ttcgcgccga cgcgggccgc
     2161 ctacaagaac ctgatgcggg agggcgtgcc gtccgagcgc gtcgtcatca ccggcaacac
     2221 cgtcgtggac gcgctgcact cgatcatgcc gcaggacgcc gcctcacggc ggcgcggcga
     2281 ggtcgtgcgc gtgcttgcga ccgcgcaccg ccgcgagaac tggggcggcc cgctgggcga
     2341 gatctgccgc gcgctgcgcg acatcgccga agcgcacgcc aacgtcgaga tcctcttcgt
     2401 cgtgcatccg aatccgatcg tgcggcgcgc tgcggaagag gcactcggcg ggctcgagaa
     2461 catccgtatc tcgccgccgc tcgcgtaccg cgacttcgtc gcgcatatgg catcggcgaa
     2521 cttgatcctg accgactcgg gcggcatcca ggaggaggcg ccgaccttcg gcacgcccgt
     2581 ggtgatcctt cgcgagacga ccgagcgcgt tgagggcatc gagagcggca gcgccatcat
     2641 tgcgggcacg acccgcgact cgatccgcgc cgccgcgttc gcggcgctcg cgcgcggcga
     2701 aggcaagccc aagacccgga cgctttacgg ggacggcaag gcgtccgagc ggatcctggc
     2761 ggcgctcatg gaccggttct tcggcagcgc cgaggaagag atcgaggaga ccggaagtgc
     2821 cctcgacggc gtcagggcgt agcgcaccgg catcgcgcag agcgggccat cggcccgctc
     2881 ttttcttgtt cgcggaagga atgacccaat cgcgatgaat atctccgccg gcttgcggcc
     2941 cgtgcggcag ccgtcgcacc gcgccctgca tcgcttcccg ttcgacaagc gcgccaccgt
     3001 cgcggggctg gccgtcgcga cggcgctcgt cgcggcgtcg ctgctcttcg cgaacgcctt
     3061 gtggatcttc gaggcggatg cgctcggcgc gctgctgcga atgagcgaca cgcccttctc
     3121 cgtggccggg gagcccgccc gcctgctcgg cggcgcggtc gcgggcggcg cccggttcac
     3181 cgtgcccggc accgggtcgc cgatcccggc cggcgccgcg ctcggcggta tcgcggcgtt
     3241 cgttgcggca tcgatcgtgg cgatccggtg gcggcgcgtg ccggtgcccg ccaaggtgct
     3301 gtggctgctg ttcgccgcga tcggctcggc gacgctgtgg tacaccggct tcgtctccgg
     3361 gtcgccgccg cagccggtgc gcagcctgtc gatcggtttc cagcgcggcg gcctgctgac
     3421 cgtggttgtg gcggcgctgc tgttcgcctt cgagatcttc ccggtgccgg gacgcctgcg
     3481 gaccaaggcg ggctggctgc tggggctgtg cctgttcgcg gtggcgtgga gcgtcgtgcg
     3541 catggcggtg gtgctggcga ccgcccatca catcggcccc tggaccttct tgtacctgca
     3601 ctatctggcc ggacccgtcg tggacttcct gaccatcgtc gcgttctaca gctttgccac
     3661 ctacggcctt tcgaagcggc tcgaggaggc gctggcgtga cgatcgcggt gcaggttctc
     3721 agcttcgcga tcttcgtggt ggtgacggcc tatgccgtgc gtcactaccg cttcctgtgg
     3781 aaccggatgt tcgagcggca agaccgggcc tatcaggacc tcgccggctc gtatctgccg
     3841 agcgtgaccg tgatcatccc gatgcacaac gagtcgcgcg tcgccgccgg cgtgatcgag
     3901 catctcgtcg cggccgacta cccgcaggag ctgctccaaa tcctggccgt caacgatcac
     3961 tcctccgacg acaccgccga gatcatcaac gcggtcgcgg cccgctaccc caacgtcacg
     4021 cccgtgcacc gcaccgatcc gggcgaggcc cgtggcaaga ccgaggcgct caagacgggc
     4081 acggccatgg ccaccggtga ggtcatcctg gtcttcgacg ccgactaccg gcccggacgc
     4141 gggctgctca agcggctcgt cgcgccgttc tgcgatcccg gcgtcggtgc cgtgatgggg
     4201 cgcgtcgtgc cgtcgaactc ggccgccaac gcgctcacgc ggctgctcga tctggagcgc
     4261 gccggcggat accaggcgaa cctgcaggcg cggcagaacc tcgggctgct cgtccagtac
     4321 ggcggcaccg tcggcggggt gcggcggagc gtgctcgagg ccgtcggcgg ttgggtgccg
     4381 cacctgaccg aggacaccga cctgacgctt cgcatctacc tgcgcgggtt ccgggtggtc
     4441 tacgtcaaca tcgccgagtg ttacgaggag gtcgtgcagg actgggccgc gcgcaccaag
     4501 cagctgtcgc gctgggccgt cggccacaac atggtcatgc tcgactacct cgggccgacg
     4561 ctgcgctcga aggtgctcac gccgcggcag aagatcgaag gagtgctgct gctgggcgtc
     4621 tacctcgcgc cggtaatgct gctcgccgga tggatcgctt cgatctacct cgcgttcgtc
     4681 tccagcggat ggttcctgac cgtctacacg gggattctcg ggtcgctgtc gttcaacgcc
     4741 tggggcaacc tcgcggcctt caacgagatc ggcagcgcct tgctgctgga tcgacgcacg
     4801 cggagcatcc tgctgctgcc gctcgcgatt ttctcgtcgt tcgcgggcat gtggatctgt
     4861 accggcgcgc tgatgaagac cgtcaaggcc cgcatccgaa agaccggcct cgcctgggac
     4921 aagacgcctc ggtacacgca ggacgccgcg cccgcccccg ccgagccgga gacctcccga
     4981 tgagcctcgg ctacgtcgcg ctcgtcggca tcttcttcgc gatgatcgcc tccctgttcg
     5041 cgatggcgct cgcggagatc cggcgcccgc gcgaccccgg gccgctcccg atcgacgtgg
     5101 gacgcgacat cgagcaccgg tggtttgcgc gcgtgctccg cggcctgccg atgggggacg
     5161 tggcgaccgc cggcgacgcc ggcgcggccg atgcgccgct gctgatattc gccgcgccga
     5221 ccggcgaagg gacctacatg atgcgcggcg acggcgacgt accgggcggc ggcgtcctgg
     5281 acgcgttcgt ctgcgacggc atcgcgaccg tcggaccggg cgccgccgtg ctcgagcatg
     5341 tcgacggcga ggccggactc gtcgtcggtg ccggggcctc cgtcggcggg cgcgcgacga
     5401 gcgcgacgtg gatcctgctc gcaccgggct cgcacgtgcg ctacgcggcc gcgccccaga
     5461 tcatgaccga cgggcccacg gtgcccgagg accccgcggc cctcgccgtc tgggacgggt
     5521 cgatggacgt ggacctggag tggctgatca cccaaggaat gcccgtcgat caggcgcttc
     5581 gctcgctgtc gcgcgatcaa ggccggccgc tcgacgggat tgccgcggtg agtgcgctcg
     5641 aagcccgcac cgggatcggc gccgccacgc tcgacctggg cgcggcgatc aatccgtggt
     5701 ggctgcgcga cgcggaaggc tggtacctcg gcgcggccac ggcacgcatc ccgggcgacc
     5761 tggcgttacc ggccggcgcg gtcgtgccgt tccacctcat cgtggacggc accctggtgg
     5821 cccgcgaggg cgtgcacttc cacggcggcg tgtccgtgcg cggggacgcg gtcctgttcg
     5881 acggctgccg cgtcgagcgc tcgctcaccg cgaaaggcaa cgtgctgctc agcgaccgtt
     5941 gctcggtggg ggagtgcgtc tcggccggtg gggacgccac cctcggcgcc tccgtcctga
     6001 tcgggggcgt gcgcaacggc gggcttgcct ctacgggctc ggtcgcgctt ggcccgggtg
     6061 ttcgcgtcac caacaagatc tacgccgacg gcggcatcgt cgtgctcgcc ccggacgcgg
     6121 ccgctctgta gccggacggc ctaccagccg cgttcgcgcc acacctcgac gtgcggccgc
     6181 tcgcggccga tcgtggtgtc gaggccgtgg cccgggcaca cgcgcgtgtc gtcgggcagc
     6241 gtgaacaggt gctcgtcgag ctgctgcatg atctgcgcga acgccgccgg gttgccgcgg
     6301 gtgttgccgg gcccccccgg aaacagcgtg tcgccgctga acagaaacga tcccgcgagg
     6361 aagcagaccg accccggcgt gtgccccggc gtgtgcagca ccgttacatc ggtctcgccg
     6421 acccgaatcg tctcgccgtg cgacagcggg accgtcgtga ccggcatcgg atccgccggg
     6481 tgggcccgca ccggacagcg gaaccgctcg acgagcgaag cgagcgcctg cacatgatcg
     6541 aagtggccgt gcgtctggac gatgccgacg acctcgtcgt cgccggccat ggcggcgatg
     6601 cggtcgggct cgtcggcgcc gtccacgatc agaacctgcc ccccggatcg cagcgcatag
     6661 acgttgttgt cgtaggcccc gacggtgatc ttgcggacct cgaacgaggc gccggacggg
     6721 tagacgtgcg gcgcggcgtt gcgggtcacg gagccgaact cgggcgtcgc gggcggatgg
     6781 ggcgtttcca tgagatcgga tcctatcgct cgctcgccgc ttgctccgga aaacgcccgt
     6841 attcgtgtcg gcggtccctg ttaggattcc ccgcgctttc gggggcgggt gcgatcggga
     6901 agaacaggac atgagcagcg ataagatcgt cgtgcacggt gctcgcgagc acaatctcaa
     6961 ggacatccat ctcgagttcc ctcgggacgc catggtcgtg ttcaccggca tctcgggctc
     7021 gggcaagtcc tcgctcgcct tcgacaccat ctacgcggaa gggcagcggc gctacgtgga
     7081 gtcgctgtcg tcctacgccc gccagttcct cgggcagatg gaaaagcccg acgtggactt
     7141 catcgaaggc ctgtcgcccg ccatctcgat cgatcagaag accgcgggcc acaatccgcg
     7201 ctcgaccgtg ggcaccgtca ccgagatcta cgactacctt cgcctgctgt acgcgcgcat
     7261 cggcgtgccg cattgcccca cgtgcggccg cgaggtcgcg cgacagacac ccgaccagat
     7321 cgtggaccag atccacgcgc tgcccgaggg aacccgcttc caggtgctcg cgccggtcgt
     7381 acgcgaccgc aagggcgagt acgcgacgct ggtcgaggac ctgtccaaga agggctttgc
     7441 gcgcgccaag atcgacggcg aggtgcgcga gctcagcgac aagatccggc tcgaccgcta
     7501 ttacaagcac accatcgagg tcatcgtcga ccggctggtg atgaagcccg gcatcgagcg
     7561 ccgcgtcgcc gacagcgtcg agaccgcgct gaacctcgcc gaagggctcg tgtcgatcga
     7621 gctcgtcgac cacggcgacg tgcaggtcta cagccagcac ctcgcgtgca tgcactgtgg
     7681 tctgtccttc gacgagctgc agccgaagaa cttctcgttc aacagcccct acggcgcgtg
     7741 cgagcgctgc gacggtctcg gaacgcacct ggaggtcgat cccgagctga tcgtgcccga
     7801 cgaggatctg tcgatcgagg aaggcgcgct cgcgccgtgg gcgagcggcc gtaccgagta
     7861 tttcgcgcgt atgctcgagt ccgtcgccgc ctacgcgaag gtgagcatca agaagccgtg
     7921 gaagtcgctg accaagaaac agaaggacat cttcctctac ggcaccggcg acgacacgat
     7981 ccacgtcaag tatcgcaacc gctacggccg cagccgctcc tacgacacct actacgaagg
     8041 cgtgatcggc tggctgcagc gcaagcacga cgaggtggac agcgactacc accgcgaaaa
     8101 gctcgaggag tacatgcggg agatcccctg ccccgcctgc aaagggtcgc gtctgaagcc
     8161 cgagtcgctg tcggtcgcca tcggcggcaa gaacatcgcc gaggtcacct cgatgtcgat
     8221 cgccgaggcc gacaagttcc tgggaacctt ggagtgggac gagcgcgagc acatgatcgg
     8281 ggaccggatc atcaaggaga tcgcggcgcg cctgggcttc ctgctcgacg ttgggctcga
     8341 ctatctgacg ctcggccgga gcgcgaacac gcttgccggc ggcgaggcgc agcgcatccg
     8401 gctcgcgacc cagatcggca gcggcctcgt cggcgtgctc tacatcctgg acgagcccag
     8461 cgtcgggctg caccagcgcg acaatcaccg tctgatcgag acgctcgttc gtctgcgcga
     8521 catcggcaac acgctcgtgg tcgtcgagca cgacgaggcg acgatcgcga ccgccgacta
     8581 cgtcgtggac atcggccccg gcgccggcga gcacgggggc agcctcgtct acgccgggga
     8641 ctacaagggc ctgctcaaga gcaaggagtc gctgaccggc cagtacctgt ccggtcgccg
     8701 gtcgattccc gtcccgggca tgcgtcggcc gcccggcggc aactggctcg gcctcaaagg
     8761 cgcgcggcag aacaacctca agaacatcga cgtcaagatc ccgctcgcgt gcttcgtggc
     8821 gatcacgggc gtgagcggca gcggcaagtc gagcctgctc accgacacgc tctatcctgc
     8881 gctgatgcgg cacgtgtacg cgtcccgcac cgtgccgggc aagcacaagg cgctcaccgg
     8941 gatccaccac atcgacaagg tcgtcaacgt cgaccaggcc ccgatcggcc gtacgccgcg
     9001 cagcaacccc gcgacctaca tcggcgtgtt cgaccacgtc cggtcgctgt tctccaagac
     9061 gcccgaggct cgcagccgtg gctaccagcc cggccggttc tcgttcaacg tcaagggcgg
     9121 ccgctgcgaa gcctgcaccg gtgacggcca gatcaagatc gagatgcact tcctgccgga
     9181 catctacgtg ccgtgcgaga tctgcaaggg caagcgctac aaccgcgaga cgctcgacgt
     9241 gacgttcaaa ggcaagaaca tctcgcagat cctggagatg agcgtggaag aggcgctgtc
     9301 ggttttcgag gccgtgccgg tgatccaccg gcatctgcag acgctgttcg acgtcggcct
     9361 tggctacgtc aagctcggcc agcccgccac gacgctgtcc ggcggcgagg cgcagcgcgt
     9421 caagctcgcg tcggagcttt cgaagcgcgc gaccggacgc acgctctaca tcctcgacga
     9481 gcccaccacc ggcttgcact tcgaggacat caacaagctg ctcggcgttc tgcagcggct
     9541 cgtcgaccag ggcaacacgg tcgtggtgat cgaacacaac ctcgacgtga tcaagaccgc
     9601 cgattggatc atcgacctgg ggcccgaggg cggcagcggc ggcggcgaga tcgtggccga
     9661 aggcacgccc gaggacgtcg ccaaggagcc gggcagctac accggccggt tcctgcgcga
     9721 agccctgggc ctgccggccg acgacgccgc gaccggctaa cgtttggcga acgcgagcgc
     9781 ggtcagcaga tacggatcgt ccgaaaccgc ggtgtctgcg tcgaaccgcc aggtccctcc
     9841 ggtcccgttg cgaaccggga tcgacggata caggccggca tcgctgcgca ccgatccatc
     9901 cggcgccgtc accgacagcg cgccggaacg cgcgctgaac acgcccagca gatacggcga
     9961 ggaagccgcg aacgatccgt gcacaagggt cgcgccttgc gccgcgacgg acctcgggaa
    10021 ctggcgcgcg cccgcggtca gcacccgggc atcgaccccg gaggccgtga cgtcggcgcc
    10081 gttcaggatg gccgagatct ccgtaaccgt tccttccgtc gcgccgcatc cgccgcgcag
    10141 cgtcgaggcc cacaggcggt gcccgcctcc ccaagtggcc gtcatcgtga tccggtagcg
    10201 ctcgcccacg gtcacgacct cgaccggggg agcctcgatc cggatgccgt cgcggcggac
    10261 gacgaggtcc aagcggccgt ggtcccacca gcccagcgcc gtgaccgacg actcgacgta
    10321 tccggtggag tccgcatcga ccgcgaatgc cgcgatccac gcgccgctcg cggcggccgg
    10381 gctgatcgtg cagcgcacga gaaccgagac cgggccgtga ggcacaggta cctgcaccgc
    10441 caccgccgcg tctcgcgctt cggcgcgggc gggtcccgca gcgagcagcc agaccgagca
    10501 caaacccgcc atccagaccc gaagccccat cggagcaaag tagagcaacc ggcaaaatgc
    10561 gtcaatagag aagaatattc cccaccccgc ccccggacgc cccgccttgc aacttggttg
    10621 cacagggtcc ggg
//
LOCUS       DATLFO010000023         4028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_20777, whole genome shotgun sequence.
ACCESSION   DATLFO010000023 DATLFO010000000
VERSION     DATLFO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20777"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..984)
                     /locus_tag="VM841_00850"
     CDS             complement(70..984)
                     /locus_tag="VM841_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL88760.1"
                     /translation="MKILIAGASGTLGARRAHRLLSGGHDVWGTTRGRIDVLASAGVR
                     PVVADLLDPAATREAVASVAPEAIVQVLNALPPAGPSKASDLLATNRIRADGTRNLVA
                     AAEASGVGRYVAESFVLGYGSRPPGSPPLDESAPLARGLPDGMQDAVDALAGLEQRVL
                     GFGGIVLRFGVFYGHGVPSNDALAAAVRKRGVPVFGFPGVMPFVHIDDAAEAVAIALG
                     SAQRGEIYNIAGTHATFGEYVTALAAATGAPSPRRLPRFAARLGGAYRASMLKANLRV
                     ATNKAFTGLGWRPSHPTLREGLAMPARR"
     gene            complement(981..1361)
                     /locus_tag="VM841_00855"
     CDS             complement(981..1361)
                     /locus_tag="VM841_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88761.1"
                     /translation="MSATKGFSVGAGAVLAGLGLWAFFSPATFYEFATYPPYNPHFIR
                     DVGAFMTGLGAVLILAGFFADALTVALGGNTVGAAVHGISHLIDRELGGGSWRDPVLT
                     LGFAAVLGFAVFKRRQAIAEGAPR"
     gene            complement(1396..2289)
                     /locus_tag="VM841_00860"
     CDS             complement(1396..2289)
                     /locus_tag="VM841_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVL88762.1"
                     /translation="MRKGILTGTSSWTDPTLLKSGWYPKEAKTAEERLVFYSSHFPVV
                     EVDATYYAMPSERTAGLWVDRTPKQFVFDVKAFALFTQHPANVRGLPKDLREGLPPTR
                     SKVYLKNLAPEVIDEAWERFRSALMPLHSAGKLGAVLFQFPEWFAPSVPNKAYLASLR
                     ERLPDYDIAVEFRRNTWMGTPEGADKTLAYLQELGYSYVCVDMPQGFDSSLPPIAAAT
                     SSSLAIVRFHGRNRKTWKAPGLTAAQRFNYLYKESELEEWEPKLRELAGEAREVHALF
                     NNCYADKGVRNARQMAELIED"
     gene            complement(2286..3893)
                     /locus_tag="VM841_00865"
     CDS             complement(2286..3893)
                     /locus_tag="VM841_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVL88763.1"
                     /translation="MPGYNIPDVLVVGMGGGGAVVAKELAEQGLGVVVLEAGAWLEPA
                     RDFAGLEWEMTGQIDSVFRWGPVDRTRLPWPRILDGVRSATQTAGVGGNTLQSAGSSP
                     RAYPGSVTPGWPLDYGDLIPHYEKVEATLPVVVPDVIAPKDQLFIEGCERAGLAHVAG
                     PDVTGPGWRIQPNAILPVASRHTAADGCTQCGGCLTGCRNPAGSRAAHTAKRGANASY
                     APLAMATDNCEIRTGCFATRVLTEERGGRVRVRAVRYRDAAGKMFEQDAEVVVLAGGA
                     VETPRLWLASGLPVGPHTGRGLTTHWFEYVVGVVPRETGPDLGQASMARADFPGEGFL
                     QSLGAGPLATALLAYGGAWPRPSTGPWATRGKMLGPDLKRRMEAYRHTLLIACGVNDD
                     PSPESSVTLSGDVRDEHNPAPVIRYRASAGTAARRDRLAKRAAEILLAAGAIPESIHR
                     ADAPPALVHQMGTMRMSASPEDGVVDGACEAHGVDGLFVADASVLPDALGGASPTLTI
                     QALATRTAGKIAERHFGALERRIVRGR"
BASE COUNT          598 a         1484 c         1388 g          558 t
ORIGIN      
        1 gacgcctggt tcgccaccac cgagatcgac gagggcgaac ggcccaacat cgacaagctc
       61 gagcgctgat cagcgacgcg ccggcatcgc gagcccctcg cgcagggtcg gatgcgacgg
      121 ccgccagccg agcccggtga atgccttgtt cgtagcgacg cgcaggttcg ccttgagcat
      181 cgacgcccgg tacgcaccac ccagccgcgc cgcgaagcgc ggcagccgcc gcggcgacgg
      241 cgcgccggtc gcggcggcga gcgcggtcac gtactcgccg aaggtcgcgt gcgttcccgc
      301 gatgttgtag atctcgccgc gctgcgcgct gccgagcgcg atcgcgaccg cttcggccgc
      361 atcgtcgatg tgcacgaacg gcatcacgcc cggaaagccg aacacgggaa cgccgcgctt
      421 gcgcaccgcc gcggcgagcg catcattgct cggcaccccg tggccgtaga acacaccgaa
      481 acgcagcacg atccccccga agccgagcac gcgctgctcg agcccggcga gcgcatccac
      541 cgcgtcctgc atcccgtccg gcaggcctcg cgcgagcggg gcggactcgt ccagcggcgg
      601 cgagccgggc ggcctggacc cgtagccgag cacgaacgac tcggcgacgt atcggcccac
      661 gccgctcgcc tcggcggccg cgacgaggtt gcgggtcccg tccgcgcgga tgcggttggt
      721 cgcgagcaga tccgacgcct tcgacggccc cgcgggcggc aacgcgttca gcacctgcac
      781 gatcgcctcg ggagccaccg acgcaacggc ctcgcgggtc gcggccggat caagcagatc
      841 ggccaccacg ggccgcaccc cggccgacgc gagcacatcg atgcgtcccc gcgtcgtgcc
      901 ccacacgtcg tgcccgccgc tgagcaaccg gtgcgcgcgt cgcgcaccga gcgtgccgct
      961 cgcaccggcg atcaggatct tcatcgtggc gctccttccg cgatcgcctg gcggcgtttg
     1021 aagaccgcga aaccgaggac cgctgcgaag ccgagcgtca ggaccgggtc gcgccacgag
     1081 ccgcctccga gctcgcggtc gatcagatgc gagatcccgt gcaccgccgc tccgaccgtg
     1141 ttgccgccca gcgcgaccgt cagcgcatcg gcgaagaatc cggccaggat cagcacggct
     1201 cccagcccgg tcatgaacgc gcccacgtcg cggatgaagt gggggttgta gggcggatag
     1261 gtcgcgaact cgtagaacgt ggccggggag aagaacgccc acaggccgag cccggccagg
     1321 acggcgcccg ctcccaccga aaatcccttc gttgcgctca tcgtcatccc tcctgtcctc
     1381 atgacccggc gtggatcagt cctcgatcag ctcggccatc tggcgggcgt tgcgcacgcc
     1441 cttgtccgcg tagcagttgt tgaacagcgc gtgcacctcg cgcgcttcgc cggcgagctc
     1501 gcgcagcttc ggctcccact cctccagctc cgattccttg tagaggtagt tgaagcgctg
     1561 ggcggcggtc aggccgggcg ccttccaggt cttgcggttg cgtccgtgga agcgaacgat
     1621 cgccagcgac gacgaggtcg cggcggcgat gggcggcagc gacgagtcga agccttgcgg
     1681 catgtccacg cacacgtacg agtagccgag ctcctgcaga tacgccagcg tcttgtcggc
     1741 gccctcgggc gtgcccatcc aggtgttgcg gcggaactca acggcgatgt cgtagtcggg
     1801 caggcgctcg cggaggctcg ccaaatacgc cttgttgggc accgacggcg cgaaccattc
     1861 ggggaactgg aagagcaccg cgccgagctt tccggccgag tgcagcggca tcagcgcgct
     1921 gcggaatcgc tcccacgcct cgtcgatcac ctcgggcgcg aggttcttta gatacacctt
     1981 cgagcgtgtc ggcggcagcc cttcgcgaag gtccttgggc aggccgcgca cgttggcggg
     2041 atgctgcgtg aacagcgcga aggccttgac gtcgaacacg aactgcttcg gggtgcgatc
     2101 gacccacagc ccggcggttc gctccgacgg catcgcgtag taggtggcgt ccacctcgac
     2161 gacgggaaaa tgcgacgagt agaagacgag ccgttcctcg gccgtcttgg cctccttcgg
     2221 gtaccagccc gacttcagca gcgtcggatc ggtccacgac gacgtgccgg tcagaatgcc
     2281 ttttctcatc gtccgcgaac gatacgcctt tcgagcgccc cgaagtggcg ttccgcgatc
     2341 ttgcccgcgg tgcgcgtggc gagcgcctgg atcgtcagcg tcgggctcgc gccgccgagc
     2401 gcatcgggca gcacgctggc gtcggcgacg aacagcccgt ccaccccgtg ggcctcgcag
     2461 gcgccatcga cgacgccgtc ctccggcgag gccgacattc gcatcgtgcc catctgatgc
     2521 accagcgccg gcggtgcgtc ggcgcggtgg atcgattcgg ggatcgcgcc cgcggcgagc
     2581 aggatctcgg ccgcgcgctt ggcgagccgg tcgcgccgcg ccgccgtgcc tgccgaggcg
     2641 cgatagcgga tcaccggcgc cggattgtgc tcgtcacgca cgtccccgga cagcgtcacc
     2701 gacgattccg gcgacgggtc gtcgttgacg ccgcaggcga tgagaagcgt gtgccggtag
     2761 gcctccatgc gccgcttcag gtccgggccg agcatcttgc cgcgcgtcgc ccacgggccg
     2821 gtggacggac gcggccacgc gccgccgtag gcgagcagcg cggtcgcgag cggcccggcg
     2881 cccagcgact gaaggaagcc ctctcccggg aagtcggccc gcgccatcga tgcctgaccc
     2941 aggtccggac ccgtctcgcg cggcaccacg cccacgacgt actcgaacca atgcgtggtc
     3001 agcccccggc cggtgtgcgg gcccacgggc aggccgctcg cgagccacag ccgcggcgtc
     3061 tcgacggcgc cgccggcgag caccacgacc tcggcgtcct gctcgaacat cttgccggcg
     3121 gcgtcgcgat agcgaacggc gcgcacgcgc acacgcccgc cgcgctcctc ggtcagcacg
     3181 cgggtcgcga agcacccggt ccggatctcg cagttgtcgg ttgccatggc gagcggcgcg
     3241 taggaggcgt tcgcgccgcg cttggcggtg tgcgcggcac gcgacccggc ggggttgcgg
     3301 catccggtca ggcatccacc gcattgcgtg cacccgtcgg cggcggtgtg gcgcgaggcg
     3361 accggcagga tcgcgttcgg ctggatccgc cagccgggcc cggtcacgtc cggaccggcc
     3421 acgtgcgcga gcccggcgcg ctcgcatcct tcgatgaaga gctgatcctt gggcgcgatc
     3481 acgtccggca ccacgaccgg cagcgtcgcc tcgaccttct cgtagtgcgg gatcagatcg
     3541 ccgtagtcca gcggccagcc cggcgtgacc gagcccggat aggcacgcgg cgagctgccc
     3601 gcggactgca gcgtgttgcc gccgacgccc gcggtttggg tcgcgctgcg cacgccgtcg
     3661 aggatgcgcg gccacggcag cctcgtgcgg tccaccgggc cccagcgaaa gaccgagtcg
     3721 atctggccgg tcatctccca ttcgagaccg gcgaagtcgc gtgccggctc gagccacgcg
     3781 cccgcttcca gcaccaccac ccccaagcct tgctcggcga gttccttggc cacgaccgcg
     3841 ccgccgccgc ccatgccgac cacgagcacg tccgggatgt tgtagccggg catcaggcca
     3901 gcagccccgg aacggattcg gcgggcccgg cgtagccgac gaggtcccag ccgaccggcg
     3961 tgccggtgat cgcgccggtc tcgtcctggc cggtccactc gccgtaatac gcagccatca
     4021 ccagcgcg
//
LOCUS       DATLFO010000024         3981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_30498, whole genome shotgun sequence.
ACCESSION   DATLFO010000024 DATLFO010000000
VERSION     DATLFO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30498"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1134
                     /locus_tag="VM841_00870"
     CDS             7..1134
                     /locus_tag="VM841_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88764.1"
                     /translation="MSSCTQPSQLRLFVDGIEGISKNGKAPSRAVASIKVDVAPDCKA
                     PPPAPPSLSFGAGRSDPDPDGSYTLKWTRPKGAVGPDEIQESRGLGTLLADGAEEGMG
                     KWARSKSSPAAFDWDTTTVKVRSGSNAFVARHPEGAANVESVMESADPMKVPSGVETT
                     LSWWDWLAAEGDDAVFVDVHDGRDWANVYQASRPLGADGAAPGLLNEPFNFHSIDLTR
                     FAGKAIKLRFRFATGPDNRALSSPMFGWYVDDIAISTAAWRTIAAVPGTSYTRANLPD
                     GEYFYRVRTAFPFGSGAKVSGPWSEVIGTKVQGSKATAYPPSGGSSPSPSPSPTSQVK
                     GVRNRAPLPATGVGGSAYGLLLVLGAAAIAIATRRPSHNGR"
     gene            complement(1206..1430)
                     /locus_tag="VM841_00875"
     CDS             complement(1206..1430)
                     /locus_tag="VM841_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88765.1"
                     /translation="MADLSFETDIKPLFRDKDRQAMMFAFDLHEYASVRDNAADILAE
                     VESGNMPCDAPWAPEQVETFRAWKDGGASP"
     gene            complement(1551..2246)
                     /locus_tag="VM841_00880"
     CDS             complement(1551..2246)
                     /locus_tag="VM841_00880"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HVL88766.1"
                     /translation="MSQDRAVYRYAVYAIDPAWRRLPAEDRRQHKKELAAALSSVDGA
                     EIYPYSTVGLREDGDFLLWTVAWDIEPVQRAQAAINACEVAGWLTVTHSFLAMTRKSQ
                     YLKGHSHDGQEHAERPGPRGDTCLFVYPMDKERRWYRVPFEERRAMMVEHFKIGHKYP
                     AVKIHTGYSFGIDDQEFVVAFEGESLAEFLELVEELRGSGASAYTARDVPIFTCVKAP
                     MDAILDQLDGAAR"
     gene            complement(2243..3508)
                     /gene="hemL"
                     /locus_tag="VM841_00885"
     CDS             complement(2243..3508)
                     /gene="hemL"
                     /locus_tag="VM841_00885"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HVL88767.1"
                     /translation="MPDALWERARSVMPGGVSSPVRAYGAVGGDPFFAADASGARVRT
                     EDGRTLIDMIGSWGPLILGHAHPAVVEAVTQAAAGGTTFGIPTRREVELAEAIVASVD
                     SIDMVRLVSSGTEAAMSAVRLARAASGRDGIVKFAGCYHGHSDAMLVQAGSGVATFGL
                     PESPGVTPGAAVDTIVARYNDASTVASAFALHQIGAVIVEPVGANMGVVPPADGFLQS
                     LRNLCDAGGALLIFDEVVTGFRLARGGAQAVYGVKPDLTVLGKVVGGGLPLAAYGGSR
                     DLMSRIAPAGDVYQAGTLAGNPLATAAGLATLALLDDDAYARLDEAGRMLEHGLRSAA
                     AEAGATVTINRVGSMLTVFFTDGPVTDFDSARRTDAKRFARFFHALLREGVAWPPSPF
                     EAAFVSLAHTPGDIGAVIAAARFGFQEAR"
     gene            complement(3501..>3981)
                     /locus_tag="VM841_00890"
     CDS             complement(3501..>3981)
                     /locus_tag="VM841_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVL88768.1"
                     /translation="DGRVAAIRRALDDAGHRSTAILSYAAKYASAFYGPFREAAGCAP
                     ASGDRRGYQIDPSSRRQGVASISRDLAEGADMIMVKPALAYLDVISAARALTDAPVAG
                     YSVSGEYAMLKAAAAAGMLDERAATLETLTAIVRAGADFVVTYSALDAARWLEDVNA"
BASE COUNT          594 a         1402 c         1391 g          594 t
ORIGIN      
        1 gagtagttgt cctcgtgcac gcagccgtcg cagctccgtc tgttcgtcga cgggatcgag
       61 ggcatctcca agaacggcaa agccccgagc cgcgccgtgg cgagcatcaa ggtcgacgtg
      121 gcgcccgact gcaaggcgcc gccgcccgcg ccgccgtcgc tgtcgttcgg cgccggccgc
      181 tccgaccccg atcccgacgg ctcgtacacg ctcaagtgga cacggcccaa gggcgcggtc
      241 gggcccgacg agatccagga gagccgcggc ctcggcacgc tgctcgccga cggggccgaa
      301 gaaggaatgg gcaagtgggc gcggtcgaag tcgagccccg cagccttcga ttgggacacc
      361 accaccgtca aggtgcgcag cggatcgaac gcgttcgtgg cgcgtcatcc ggaaggcgcg
      421 gccaacgtcg agtccgtgat ggagtcggcc gatccgatga aggtgccgtc cggcgtcgag
      481 accacgctct cctggtggga ctggctggcc gccgaaggcg acgacgccgt gttcgtggac
      541 gtgcacgacg gccgtgactg ggccaacgtc tatcaggcct cgcgtccgct gggggccgac
      601 ggcgcggcgc ccgggttgct caacgagccg ttcaacttcc actcgatcga cttgacgcgc
      661 ttcgccggca aggcgatcaa gcttcggttc cggttcgcca ccggcccgga caaccgggcg
      721 ttgtcctcgc cgatgttcgg ctggtacgtg gacgacatcg cgatctcgac cgccgcatgg
      781 cggaccatcg ccgccgtgcc gggaacgagt tatacccgcg ccaatcttcc ggacggcgag
      841 tacttctatc gcgtgcgcac cgcgttcccg ttcggatcgg gcgccaaggt ctccggcccg
      901 tggagcgagg tgatcggaac gaaggtccag ggcagcaagg cgacggccta tccgccgtcg
      961 ggcggttcgt caccgagccc gtcgccgtca ccgacctcgc aggtgaaggg cgttcggaac
     1021 cgggctccgc tgcccgcgac cggcgttggc ggatcggcct acggcctgct gctggtgttg
     1081 ggcgccgccg cgatcgcgat cgcgacgcgc cggccgtcgc acaacggcag atagcggtga
     1141 cctgatcacg gaacggcgag aggcggcgcg tgatggctgc gccgcctctc gccgttccgt
     1201 gcggactacg ggctggcccc gccgtccttc cacgcgcgaa acgtctccac ctgctccggc
     1261 gcccagggcg cgtcgcacgg catgtttcca ctttcaacct cggcgaggat gtcggccgcg
     1321 ttgtcgcgga cggacgcgta ctcgtgcagg tcgaaggcga acatcatcgc ttgccggtcc
     1381 ttgtcgcgga acagcggctt gatgtcggtt tcgaagctga ggtctgccac cggtttcccc
     1441 ccttgccgat cgaagctcct gcgaagccgc ccgatcctat cgcggcctcg cggcggggac
     1501 gaaccgggcg ccggtcagcc gggggcgcct ccttttctgt tccccggtcg tcagcgcgcg
     1561 gcgccgtcga gctgatccag gatcgcatcc atcggcgcct tcacgcacgt gaagatcggc
     1621 acgtcgcggg cggtgtaggc gctggcgccg ctcccgcgca gctcttcgac cagctccagg
     1681 aactcggcca gcgactcgcc ttcaaaggcg accacgaact cttgatcgtc gatgccgaac
     1741 gagtagccgg tgtggatctt cacggccgga tacttgtgac cgatcttgaa gtgctcgacc
     1801 atcatcgcgc gccgctcttc gaacggaacg cggtaccagc ggcgctcttt gtccatcgga
     1861 tacacgaaca ggcaggtgtc gccgcgggga ccgggccgct cggcgtgctc ttgcccgtcg
     1921 tgcgagtggc ctttcagata ctgggatttg cgggtcatcg ccaggaacga gtgcgtgacg
     1981 gtgagccagc ccgcgacctc gcacgcgttg attgcggcct gcgcgcgctg gacgggctcg
     2041 atgtcccatg cgacggtcca gagcaggaag tcgccgtcct cgcggagccc cacggtcgaa
     2101 taggggtaaa tctcggcgcc gtccaccgac gacagcgcgg ccgcgagttc tttcttgtgc
     2161 tggcgacggt cctcggcggg caggcgccgc cacgcgggat cgatcgcgta gacggcgtag
     2221 cgatagacgg cacggtcctg gctcatcggg cctcctggaa tccgaaacgc gccgcggcga
     2281 tgacggcgcc gatgtcgccg ggggtatggg cgagcgagac gaacgcagcc tcgaacggcg
     2341 acggcggcca cgcgacgcct tcgcgaagca gcgcgtggaa gaaccgcgcg aaccgtttcg
     2401 cgtcggtccg gcgggccgag tcgaagtcgg tgaccggccc gtcggtgaag aacaccgtca
     2461 gcatcgagcc gacccggttg atcgtgacgg tcgcaccggc ctcggccgcg gcggatcgca
     2521 aaccgtgttc cagcatccgt ccggcttcgt cgagccgcgc gtaggcgtcg tcgtcgagca
     2581 acgccagcgt ggccagcccg gccgcggtag ccagcgggtt gccggcgagc gttccggcct
     2641 gatagacgtc gcctgcgggt gcgatccgtg acatgaggtc gcgcgatccg ccgtaggcgg
     2701 ccagcggcag gccgccgccg acgaccttgc ccagcacggt caggtccggc ttcacgccgt
     2761 agacggcttg cgcgccgccg cgcgccagac ggaatccggt gacgacctcg tcgaagatca
     2821 gcagcgcgcc gccggcgtcg cacaggttgc gcagcgactg caggaacccg tcggccggcg
     2881 gcaccacgcc catgttcgcg ccgacgggct cgacgatcac ggccccgatc tgatgcaacg
     2941 cgaacgcgga cgcgaccgtc gaggcgtcgt tgtatcgcgc gacgatcgtg tccacggccg
     3001 ccccgggggt cacgccgggc gactcgggca gcccgaaggt cgcgacgccg gagccggctt
     3061 ggaccagcat cgcgtcggag tggccgtgat agcagcccgc gaacttcacg atgccgtcgc
     3121 ggccgctcgc cgcgcgggcc agccggaccg cgctcatcgc ggcctcggtc cccgacgaca
     3181 ccaggcgcac catgtcgatc gaatccaccg acgcgacgat cgcttcggcc agttcgactt
     3241 cgcggcgtgt cgggatgccg aaggtcgtgc cgccggccgc ggcctgcgtg acggcctcga
     3301 cgacggcggg atgcgcgtgg cccaggatca gcggtcccca cgagccgatc atgtcgatca
     3361 gcgtgcggcc gtcttcggtg cgcacgcgcg cgccgctcgc atcggcggcg aagaacggat
     3421 cgccgccgac ggcgccgtac gcgcggaccg gcgacgacac gccgcccggc atcacggatc
     3481 gtgcccgttc ccacagcgcg tcaggcattc acgtcctcca gccagcgcgc cgcgtccagt
     3541 gcggagtagg tgacgacgaa atcggcgccg gctcgtacga ttgcggtcag ggtctccagc
     3601 gtcgcggcgc gctcgtccag catcccggcg gcagcggcgg ccttcagcat cgcgtactcg
     3661 ccgctgacgc tgtagcccgc caccggcgcg tcggtcagcg cgcgcgccgc cgagatcacg
     3721 tccagatagg ccagtgcggg cttgaccatg atcatgtccg cgccttcggc caggtcccgg
     3781 ctgatcgagg cgacgccttg gcgccgcgac gacggatcga tctgatagcc gcgccggtcg
     3841 ccggacgcgg gcgcgcaacc ggccgcctca cgaaacggac cgtagaacgc gctggcgtac
     3901 ttggcggcgt agctcaggat cgccgtgctg cggtgcccgg cgtcatccag cgcgcgacga
     3961 atcgcggcga cgcggccgtc c
//
LOCUS       DATLFO010000025         2857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_119473, whole genome shotgun sequence.
ACCESSION   DATLFO010000025 DATLFO010000000
VERSION     DATLFO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119473"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VM841_00895"
     CDS             complement(<1..791)
                     /locus_tag="VM841_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HVL88769.1"
                     /translation="MIRAVILAGGEGTRLRPLTYTTPKSLLPIANVPFLQHQLTTLAR
                     HGITTATLLTGYLAEAFDPFIDAMAAEGITLEVSRETDPLGTCGAVRSIRDRLDATTI
                     VFNGDVLTDLDLTEMLAAHRDRAAALTIALKPVADAGPYGLVPTDGNGRVEAFLEKPP
                     AEVAARGGNINAGTYALEPRVLDEVPPSTMWSFERQLFPSLVESGEPVYGFVSSAYWL
                     DIGTPERYLQAHWDLIEGKSSSFRPDQAGVRGGGVLVGRDCEIDPG"
     gene            complement(788..1630)
                     /gene="rfbD"
                     /locus_tag="VM841_00900"
     CDS             complement(788..1630)
                     /gene="rfbD"
                     /locus_tag="VM841_00900"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HVL88770.1"
                     /translation="MSGAVVVTGAGGLLGRAMVDALGGRAAGFDRVALDITDPDAVAA
                     AVRGAAAVVNAAAFADVDACESEPDRAYAVNAAGPENLARACAATGAFLIHVSTDFVF
                     DGTLGRAYVETDEPSPLSVYSASKLEGEQRVAQANPQAAIVRTAWVYGPGGRTFFSKV
                     FSMAAAGGPIPATADQFGSPTWVGDLAAALSQLAVRPAAGLFHVAGGGSCSPAEFVEA
                     ALGELGLAGVAVTRVRAADLPARPARRPLATPLEGPAWIAAGFEPLRPWREALEDAAK
                     VMRP"
     gene            complement(1627..2583)
                     /gene="rfbB"
                     /locus_tag="VM841_00905"
     CDS             complement(1627..2583)
                     /gene="rfbB"
                     /locus_tag="VM841_00905"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HVL88771.1"
                     /translation="MRLLVTGGAGFIGSRFAHLAIARDHEVTVLDALTYAGTMTNLAG
                     VKDHAGFRFVKGDICDPDLVDEVLPGHDAVLNFAAESHVDRSIASPENFMHTNFLGAG
                     VLLESARRAGTGRFLQVSTDEVYGSIEIGSFAEADRLNPSNPYSAAKAGADLLALSYV
                     RTFGMPVVITRSSNAFGPHQFPEKLIPLAITNLIEGKKIPLYGDGANVRDWTFVDDIC
                     EAILLVLERGETGRIYNIAAGNERRNVDVVEQICGLSSVGGDMIEPVADRPGHDRRYS
                     IDASAVRDLGWRPSGTFDERLAETVAWYRDHRAWWEPLKARA"
     gene            complement(2589..>2857)
                     /locus_tag="VM841_00910"
     CDS             complement(2589..>2857)
                     /locus_tag="VM841_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HVL88772.1"
                     /translation="STVRGPAVIGANARIVDSFIGPYSSIGAGCVIEDSEIEHSVIMD
                     GCVIRGMHRLEDSLLGREVTVERSESKPKAYRLMVGDHGRVGVV"
BASE COUNT          433 a          976 c         1032 g          416 t
ORIGIN      
        1 cccgggtcga tctcgcagtc gcggccgacg agcacgccgc cgccgcgcac gccggcttga
       61 tcgggccgga acgaggacga tttgccctcg atcaggtccc agtgcgcctg caggtagcgt
      121 tccggcgtgc cgatgtcgag ccagtatgca ctcgacacga acccgtagac gggctccccc
      181 gattccacca gcgaggggaa cagctgccgc tcgaacgacc acatcgtgct cggcggcacc
      241 tcgtcgagca cgcgcggctc gagcgcgtag gtgccggcgt tgatgttgcc gccgcgcgcg
      301 gcgacctccg ccgggggctt ctccaggaac gcctcgacgc ggccgttgcc gtcggtgggg
      361 accaggccgt agggacccgc gtcggcgacc ggcttgagcg cgatcgtcag ggccgccgcg
      421 cgatcgcgat gcgcggcgag catctcggtc agatccaagt cggtgagcac gtccccgttg
      481 aacacgatcg tggtcgcgtc gagccggtcc ctgatgctgc ggaccgcgcc gcaggttccg
      541 aggggatcgg tctcgcggct gacctcgagc gtgatgcctt cggcggccat cgcgtcgata
      601 aacggatcga aggcttcggc gagatagccg gtgagcagcg tcgcggtcgt gatgccgtgg
      661 cgcgcgagcg tggtgagctg atgctgcagg aacggcacgt tcgcgatcgg cagcagcgac
      721 ttgggcgtgg tgtaggtgag cggccgcagc ctggtgcctt ctccgccggc caagatcacg
      781 gcgcggatca cggtcgcatc accttcgcgg cgtcctcgag ggcctcgcgc cagggtcgca
      841 ggggctcgaa gcccgcagcg atccaggccg ggccttcgag cggcgtggcg agcgggcgtc
      901 gcgcgggacg cgcgggcagg tcggccgccc tgacccgcgt gacggccacg ccggccaggc
      961 cgagttcgcc gagcgcggcc tcgacgaact cggccggcga gcacgagcca ccgccggcga
     1021 cgtggaacag gcccgccgcc ggccgcaccg cgagctgcga cagcgcggcc gcgaggtcgc
     1081 ccacccaggt gggcgagccg aactggtcgg cggtcgcggg aatcggtccg cccgcggcgg
     1141 ccatggagaa caccttgctg aagaacgtgc ggccgcccgg accatagacc caggcggtcc
     1201 gaacgatcgc ggcctgcggg ttggcctggg cgacgcgctg ctctccttcg agcttggagg
     1261 cgctgtagac cgagagcggg ctgggctcgt cggtctcgac gtaggcgcgg cccagcgttc
     1321 cgtcgaagac gaagtcggtc gagacgtgaa tcaggaacgc tccggtggcc gcgcaggcac
     1381 gtgcgaggtt ctcgggaccg gcggcgttga ccgcgtaggc gcggtcgggc tcggactcgc
     1441 aggcgtccac gtcggcgaac gcggcggcgt tgacgaccgc cgcggcaccg cgaacggcgg
     1501 ccgcgaccgc gtcgggatcg gtgatgtcga gcgcgacacg gtcgaacccg gcggcccgcc
     1561 cgccgagcgc gtccaccatc gcgcgaccga gaaggccgcc cgcgccggtc acgacgacgg
     1621 ctccgctcat gcgcgcgcct tcaacggctc ccaccaggcg cggtgatccc gataccaggc
     1681 cacggtctcg gccaggcgtt cgtcgaaggt accgctgggc cgccagccca gatcgcgaac
     1741 ggcggacgcg tcgatgctgt agcggcggtc gtggccgggc cggtccgcaa cgggctcgat
     1801 catgtcgccc ccgacgctcg acagcccgca gatctgctcg accacatcga cgtttcgccg
     1861 ctcgttgccg gcggcgatgt tgtagatgcg gccggtctcg ccgcgctcga gcacgagcag
     1921 gatcgcttcg cagatgtcgt cgacgaacgt ccagtcgcgc acgttggccc catccccgta
     1981 cagcgggatc ttcttgcctt cgatcaagtt cgtgatcgcg agcgggatga gcttttcggg
     2041 aaactggtga gggccgaatg cgttcgacga gcgcgtgatc acgaccggca tgccgaatgt
     2101 gcgcacgtac gacagcgcga gcaggtccgc gcccgccttg gcggcactgt aagggttcga
     2161 tgggttgagc cggtccgcct cggcgaagga gccgatctcg atcgagccgt aaacctcgtc
     2221 ggtgctgacc tgcagaaagc ggccggtccc ggcgcggcgc gcggactcga gcagcacgcc
     2281 cgcaccgagg aagttcgtat gcatgaagtt ttcggggctt gcgatcgagc ggtccacgtg
     2341 cgactcggcg gcgaagttca gcacggcgtc gtggccggga agcacctcgt cgaccagatc
     2401 gggatcacag atgtcgccct tgacgaagcg gaagccggcg tggtccttta cgccggccag
     2461 gttggtcatg gtgcccgcat aggtgagcgc gtccaggacg gtgacctcgt ggtcgcgcgc
     2521 gatcgcgaga tgcgcgaagc gcgagccgat gaagcccgcg ccgccggtga ccagaagccg
     2581 catgcccgtc agactacccc gacgcgtccg tggtccccga ccatcagccg ataggccttc
     2641 ggcttcgatt cgctgcgttc gacggtgacc tcgcggccga gcagcgagtc ctcgagccgg
     2701 tgcatcccgc ggatcacgca accgtccatg atcaccgagt gctcgatctc gctgtcctcg
     2761 atcacgcatc ccgcgccgat cgaggagtac gggccgatga acgagtccac gatgcgggcg
     2821 ttcgcgccga tcacggcggg cccgcgtacg gtcgatc
//
LOCUS       DATLFO010000026         3827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_109844, whole genome shotgun sequence.
ACCESSION   DATLFO010000026 DATLFO010000000
VERSION     DATLFO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109844"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1825)
                     /locus_tag="VM841_00915"
     CDS             complement(158..1825)
                     /locus_tag="VM841_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVL88773.1"
                     /translation="MKQLPELLLEEGLISAEQLEQAAAEQARSRMSLSRVLIDRGFVP
                     EQHLVKTLARHIGLEFVDLGEHQIDPGAVALISENVAKRYGALPIGFDDARLVVAMSD
                     PSNVFALDDIRTITGLEVKPVIATRSDILSALARYGRMDESVEDIAAAAADETDGPED
                     LSKVREIVEDAPIVKLVNLLVTQAVNDRASDIHIEPGERDVRIRYRIDGVLHEVMRSP
                     KSIQSGVISRLKIMSDINIAERRIPQDGRVGLVVGGKAIDLRVATLPTVYGEKVVLRI
                     LDKSSVLLKLTDLGFSPHNFTRYEQSYTKPYGMILVTGPTGSGKSTTLYATLNILNTP
                     DRNTITVEDPVEYRLAGINQVQINPKAGLTFASALRSILRSDPDVVLIGEIRDRETAQ
                     IATEAALTGHLVLSTLHTNSAPAAISRLIEMGIEPFLVSSAVDCVLAQRLIRVLCDKC
                     KEPYEPTPDMLEQSRIKVPEDRFPLTLYRAVGCQACGKTGYRGRMAVHEVMNMSEDIE
                     RLTSERKSSEDIARVAIDQGMLTLRDDGVLKALDGRTSIEELLRVIV"
     gene            complement(1913..2758)
                     /locus_tag="VM841_00920"
     CDS             complement(1913..2758)
                     /locus_tag="VM841_00920"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HVL88774.1"
                     /translation="MSGSPTGATRVACIIGWPASHSLSPVIHNAAFAAAGLDWVYVAF
                     PVAPDDVAAAVTGMRALGIAGANVTMPHKRAVMALMDELTPAAERIGAVNTIVANGGT
                     LIGDNTDGAGLVAFLTGEAGFDPAGRRVTLLGAGGAARAVAVALADAGSRVRIAARRA
                     GAVSEVAALCAGEVVPWDARGEAAAQSDLIVNATPVGADGEGSPLEAGDLPTTATIVD
                     LLYSPAETPLLRAAAARGLPAHNGLGMLVHQAALAFEQWTGTPAPVDAMAAAVRARTA
                     AKPSL"
     gene            complement(2755..>3827)
                     /gene="mltG"
                     /locus_tag="VM841_00925"
     CDS             complement(2755..>3827)
                     /gene="mltG"
                     /locus_tag="VM841_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVL88775.1"
                     /translation="PPVTDAPPSPDPERRSRRRRGLVKLAAFFLVLFGLLGGTALYVR
                     SVAAGTAGAGAPVKIVVPEGASAGQIAELLAGKDIIDNAWIFKLFARFDGRAGSLKPG
                     EYDMQTGMSFGEVLRTLEDGPVIRIERVTIPEGKTVGEVVDIIRTKTSISATEFKAEI
                     DSGRHRLPIMPDNVKSLEGLLYPKTYDIREGATAGQVLKMMLDQFTDETRALDFSKAK
                     ANLTPYQVVIAASLIEREAKIDEDRPKIAQVIYNRIARGMRLQIDATVQYGYLMKTGS
                     YKGRLTYADYELDHPYNTYRIDGLPPGPIASPRLASIVAMLEPADTDAIYYIHCDKRG
                     GHAFAKTASEFEKLKRECPGKR"
BASE COUNT          570 a         1226 c         1378 g          653 t
ORIGIN      
        1 gatgacacgc tcctcgacca gaacttcgcc gagcggcttg ggtgcgggca cgattcctcc
       61 gggttcgact tcgtccctgg caatgtcggc gcgttcaggg gcgtccttta cggccgcagc
      121 ctcatgccgc gaaacgaccg aatcgggcgc gggtggatca gacgatgacg cggagaagct
      181 cttcgatgct ggtgcgtccg tcgagcgcct tgagcacgcc gtcgtcgcga agggtgagca
      241 tgccctggtc gatcgcgacg cgagcgatgt cctcgctgga tttgcgctcc gaggtgagcc
      301 gctcgatgtc ctcggacatg ttcatgacct cgtggacggc catgcggccg cgatagccgg
      361 tcttgccgca cgcctggcag ccgacggcac ggtacagcgt cagcgggaaa cggtcctcgg
      421 gaaccttgat gcgcgactgc tcgagcatgt cgggcgtcgg ttcgtagggc tccttgcact
      481 tatcgcacag cacgcggatc agacgctggg ccagcacgca gtcgacggcg ctcgagacca
      541 ggaacggctc gatccccatc tcgatgagcc ggctgatcgc ggccggtgcg ctgttggtgt
      601 gcagcgtcga aagcacgaga tggccggtga gcgcggcctc ggtcgcgatc tgggcggtct
      661 cgcggtcgcg gatctcaccg atcaggacca catccgggtc ggagcgcagg attgaccgga
      721 gcgcgctcgc gaaggtcagg cccgctttgg ggttgatctg cacctgattg atcccggcca
      781 ggcggtactc caccgggtcc tcgaccgtga tcgtgttgcg atccggcgtg ttaaggatgt
      841 tgagggtcgc gtagagcgtc gtggactttc ccgagcccgt cgggccggtg accaggatca
      901 tgccgtaggg cttggtgtag gactgctcgt agcgcgtgaa gttgtgcggc gagaatccga
      961 gatcggtgag tttgagcagc acgctcgatt tgtccaggat tcgcagcacg accttctcgc
     1021 cgtagaccgt cgggagcgtg gccacgcgca ggtcgatcgc cttgccaccc acgaccaggc
     1081 cgacgcggcc gtcctgcggg atccgccgct cggcgatgtt gatgtcgctc atgatcttga
     1141 gccgcgagat cacgcccgac tgaatcgact tcggcgagcg catcacctcg tggagcacgc
     1201 cgtcgatgcg gtagcggatc cgtacgtccc gctcccccgg ctcgatgtgg atgtcggagg
     1261 cgcggtcgtt gacggcctgc gttaccagca ggttgaccag cttgacgatg ggcgcatcct
     1321 cgacgatctc gcggaccttg gacaggtcct cgggcccgtc ggtctcgtcg gcggccgcag
     1381 cggcgatgtc ttcgacggac tcgtccatgc ggccgtagcg ggcgagagcg gacaagatgt
     1441 cgcttcgggt cgcgatcacc ggcttgactt cgagcccggt gatggtgcgg atgtcgtcga
     1501 gcgcgaaaac gttggacggg tcgctcatcg caacgaccag ccgggcgtcg tcgaagccga
     1561 tcgggagggc gccgtatcgc ttggcgacgt tctcggagat gagcgcgacc gcacccgggt
     1621 cgatctgatg ctcacccagg tccacgaact cgagcccgat gtggcgggca agggtcttga
     1681 ccaggtgctg ctcgggcacg aacccccggt cgatcaggac gcgagacagc gacattcgag
     1741 agcgcgcctg ctcggcggct gcttgctcga gctgctcagc gctgatgagc ccctcttcga
     1801 ggagcagttc ggggagctgt ttcagtcgtt ttgcggccac gtcggaaggg ggcggccggt
     1861 ggtcactccg gatttccgtc ccgtcacgaa tatcgggcga cggagggccc gactataacg
     1921 acggtttcgc ggccgtccgg gcccgcaccg cggcggccat tgcgtcgacg ggagccggag
     1981 tcccggtcca ttgctcgaaa gcgagcgcgg cttggtgcac cagcatgccg agcccgttat
     2041 gagcggggag cccgcgcgcc gccgcggcac gcaaaagggg cgtttcggcc ggggagtaga
     2101 gaagatcgac gatcgtggcc gtcgtcggga gatcgccggc ctcgagcggc gagccttcgc
     2161 cgtccgcccc gactggagtg gcgttgacga tcaggtccga ctgggcggcc gcctcgccgc
     2221 gggcgtccca cggcacgacc tcgcctgcgc acagcgcggc gacctcggag accgccccgg
     2281 cacgccgtgc ggcgatgcgg acccgcgaac ctgcgtcggc gagcgcgacc gcgaccgcgc
     2341 gcgcggcgcc gcccgcgccc aagagcgtga cgcgccggcc ggcggggtcg aatccggcct
     2401 cgccggtcag gaatgcgacc agcccggcgc cgtcggtgtt gtctccgatc agcgtgccgc
     2461 cgttggcgac gatggtgttg acggcaccga tgcgctcggc cgccggcgtg agctcgtcca
     2521 tgagagccat gacggcccgc ttgtgcggca tggtgacgtt ggcgccggcg atgccgagcg
     2581 cgcgcatccc ggtgacggcc gccgcgacgt catcgggcgc gaccggaaag gcgacgtaga
     2641 cccagtcgag ccccgcggcg gcgaaggccg cgttgtggat gaccggggaa aggctgtgcg
     2701 aggcgggcca gccgatgatg caggcgacgc gggtcgcgcc cgtcggcgat ccgctcaccg
     2761 cttgcccggg cactcgcgct tgagcttttc gaactccgac gccgtcttgg cgaaggcatg
     2821 gccgccgcgc ttgtcgcagt ggatgtagta gatcgcgtcg gtgtcggccg gctcgagcat
     2881 cgcgacgatc gacgcgaggc gcggcgaggc gatcggcccc ggcggcagcc cgtcgatccg
     2941 ataggtgtta tacggatggt ccagctcgta gtcggcgtag gtcaggcggc ccttgtacga
     3001 gccggtcttc atcagatagc cgtactgaac ggtcgcgtcg atctgcaggc gcatgccgcg
     3061 cgcgatccgg ttgtagatca cctgcgcgat cttgggacgg tcctcgtcga tcttggcttc
     3121 acgctcgatg aggctggcgg cgatcacgac ctggtacggg gtcaggttcg ccttggcctt
     3181 cgagaagtcc agcgcgcggg tctcgtcggt gaactgatcg agcatcatct tcaagacctg
     3241 gccggccgtg gcgccctcgc ggatgtcgta ggtcttgggg tagagcagcc cttcgagcga
     3301 cttcacgttg tcgggcatga tcgggagccg gtggcggccg ctgtcgatct cggccttgaa
     3361 ctcggtcgcg gagatcgagg tcttcgtgcg gatgatgtcc acgacctcgc cgacggtctt
     3421 tccttccggg atggtgacgc gctcgatccg gatgaccgga ccgtcctcga gcgtgcgcag
     3481 cacctcaccg aagctcatcc cggtctgcat gtcgtattcg ccgggcttga gcgagccggc
     3541 ccgcccgtcg aagcgggcga acagcttgaa gatccaagcg ttgtcgatga tgtccttgcc
     3601 cgccagcagc tcggcgatct ggccggcgga ggcgccttcg ggcaccacga tcttcacggg
     3661 cgcgcccgcg ccggccgtgc cggcggccac cgaccggacg tagagcgctg tcccaccgag
     3721 aagcccgaaa agcaccagga agaaggcggc gagcttgacc aggccgcggc ggcggcgtga
     3781 gcggcgctcg ggatcggggc tcggcggggc gtcggtgacc ggcggct
//
LOCUS       DATLFO010000027         4549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_20937, whole genome shotgun sequence.
ACCESSION   DATLFO010000027 DATLFO010000000
VERSION     DATLFO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20937"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..964)
                     /locus_tag="VM841_00930"
     CDS             complement(164..964)
                     /locus_tag="VM841_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVL88776.1"
                     /translation="MGYRHWVFLHLAGVFGFLASHGVSMAVAIRLRKERDIARIGGLL
                     DVSSRTVGVMYASLGALLAGGIGATFAGGLWGFGWIWAALVLVAVTVLAMIFMAKPYY
                     QKVRFVTRAVAEGSKAVTGEQVDEMLRSNRPNVIMGIGFVGLALILVLMIFKPTLGIE
                     PTQAPLTMPSGPTVQVAAENSNEFVQTRVSAPAGVEFSIVFENRESGVPHNVAIYTDD
                     TAKTSVVVGELFPGPATRVMRVPALDAGTYFFRCDVHPRDMTGRLVVE"
     gene            1051..1302
                     /locus_tag="VM841_00935"
     CDS             1051..1302
                     /locus_tag="VM841_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVL88777.1"
                     /translation="MSADTPYYATARLQERLVQETGDLDVTVTVVGTVVVITGSVASP
                     EQREQITAIAREEMPSATVRNETTLPRVTESLPPETIEP"
     gene            1299..2033
                     /locus_tag="VM841_00940"
     CDS             1299..2033
                     /locus_tag="VM841_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL88778.1"
                     /translation="MIRIAAVGDLHFGTESRGCFAPHLERLGDDADLLLVAGDLTRVG
                     DPAEARVAAAELSMLPVPVVAVLGNHDYHVDRDGEVRAIFEEAGIRILEGEAAMLTVG
                     GMRVGVAGAKGFGGGFAGACASDFGEPEMKAFIRHTRATAERLESALASVDHADVRLA
                     LLHYAPVEGTLAGERPEIWPFLGSYLLAEAADRAGADLIVHGHAHAGTEHGVTPGGIP
                     VRNVAQPVLGAAYATYCLEPAPVRAG"
     gene            complement(2030..3121)
                     /locus_tag="VM841_00945"
     CDS             complement(2030..3121)
                     /locus_tag="VM841_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HVL88779.1"
                     /translation="MATPTASRSSTRSRRTAARRDAAEAGLRHVCDDDPGIRRVRSGK
                     GFRYVRAGGRPVRDRATLERIRTLAIPPAWEDVWICPDGRGHVQATGRDARGRKQYRY
                     HPRWREIRDETKYARLREFAAALPKLRAKVDRDLARSGLPRERVLAAVVRLLERTLMR
                     VGNDEYARENGSFGLTTLRAKHVTVDGEAMRFRFKGKSGKVHEIDAKDPRAARVVRKC
                     RRLPGQTLFGYLDDEGVDRTVGSGDVNEYLREATGSDFTAKDFRTWAGTVVAAIVLAA
                     ADPPASDAEAARGINEAIDEVAGALGNTRAIARTSYVHPEILEAYREGTLMEAWGRRT
                     RRIRGLSRAETIVARILRDRAGAVVEKSA"
     gene            3216..4385
                     /locus_tag="VM841_00950"
     CDS             3216..4385
                     /locus_tag="VM841_00950"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HVL88780.1"
                     /translation="MFGGGRIRAVALLVALTALIAPEARASDRASFPLPRGYTIAGID
                     HLADGVTHVRYVALSPRRNVNVAFIRPGAAARMKVVVSNDRIAGGTETVRSMCARAGC
                     IMGVNADLFSFGSGQPAGGMASQREFFKTPQSFRPHLMQAGDGSTSIGNLTTTIRFFA
                     SYPERVEPFYIHGINVPRGSGQAVLYTPLWAPTTQTGSGFEITLAPVDAEATPRFGEP
                     LRVRMVQGFGAGSSSLLPGTMVLSAHGPYATLLSNLWNDVLAGRATGDAELTFAAPPE
                     ADTFTGGWPQLLRGGHQSFTDRTWSFYSEQARTLAGRTADGTVMLAVIDGGRGSYWTG
                     VRLLDAMRFMRSMGAVDALNMDGGGSSTFVVRGRAVNHPLRGERGVASALVLVPA"
BASE COUNT          655 a         1612 c         1594 g          688 t
ORIGIN      
        1 aacgcctgcc gctcgggcct cggtcatgcc ggtcggcgcg gtggcacaca cgagcacgac
       61 gcggccgttc atacgccgtg cgatgagcgt ggccttctcg acggccttgg tggccgtctc
      121 gcttccgtcg gttccgacga cgatggtggc gtaggacatg gacctattcg accacgagcc
      181 ggccggtcat gtcgcgcggg tgcacgtcgc agcggaagaa gtacgtgcct gcgtccaagg
      241 cgggaacgcg catgaccctg gtcgctggac cggggaacag ctcccccacc acgaccgagg
      301 tcttggcggt gtcgtcggtg tagatcgcga cgttatgcgg cacgccggac tcgcggttct
      361 cgaacacgat cgaaaactcc acgcccgccg gagcgctcac gcgggtttgg acgaactcgt
      421 ttgagttctc ggccgcgacc tgtaccgtgg ggccgctcgg catcgtgagc ggcgcctgcg
      481 tcggctcgat gccgagcgtg ggcttgaaga tcatcaggac gaggatgagg gcgaggccga
      541 cgaacccgat gcccatgatc acattcggac ggttcgagcg cagcatctcg tctacctgct
      601 cgccggtgac ggccttcgag ccttcggcga ccgcgcgcgt cacgaagcgc accttctggt
      661 agtagggctt ggccatgaag atcatggcga gcaccgtcac cgcgaccagc acaagcgcgg
      721 cccagatcca gccgaagccc cacagcccgc cggcgaaggt cgcgccgatc ccgcccgcaa
      781 gcagcgcgcc gagcgaggca tacatcacac cgacggtgcg cgacgacacg tcgagcaggc
      841 cgccgatgcg cgcgatgtcg cgctccttgc gaagacggat cgccacggcc atggaaacgc
      901 cgtgcgaagc caggaacccg aagaccccgg cgaggtgaag aaagacccag tggcggtatc
      961 ccacggcccg gcatcatgcc gtgccggagg cgcgcccgcc actcggctcc tgccgtttcg
     1021 cgcgcccgct tccacgggta cggtctcagg gtgagcgcgg acacccccta ttacgcgacg
     1081 gctcgcttgc aggagcgcct cgtgcaagag accggcgacc tcgacgtgac cgtgaccgtc
     1141 gtgggcacgg tcgtggttat caccggatcg gtcgcctctc ccgaacagcg ggagcagatc
     1201 acggccatcg cgcgcgagga gatgccgtcg gcgacggtgc gcaacgagac caccctcccc
     1261 cgggtcaccg agtccctccc gccggagacg atcgagccat gatccggatc gcggcggtcg
     1321 gggacctcca cttcgggacc gagtcgcggg ggtgcttcgc gccgcacctg gagcggctcg
     1381 gcgacgacgc cgacctgctg ctggtggccg gcgacctgac ccgcgtcggc gatcccgccg
     1441 aggcgcgcgt cgcggccgcg gagctttcga tgctgccggt gccggtggtg gccgtgctcg
     1501 gcaaccacga ctaccacgtg gaccgcgacg gcgaggtgcg ggccatcttc gaagaggccg
     1561 gtatccgcat cctggaaggc gaggcggcga tgctgaccgt gggcggcatg cgtgtcgggg
     1621 ttgccggcgc gaaaggcttc ggcggcggat tcgccggcgc ctgcgcgagc gacttcggcg
     1681 agccggagat gaaggcgttc atccggcaca ctcgcgccac ggctgagcgg ctcgagtccg
     1741 ctctggcgtc ggtggaccac gccgacgtcc ggctcgcgct gctgcactac gcgcccgtcg
     1801 agggcacgct cgcgggcgag cggcccgaga tctggccgtt cctcggctcg tacctcctcg
     1861 ccgaggcagc cgatcgcgca ggcgccgacc tgatcgtgca cgggcacgcg catgccggca
     1921 ccgagcacgg cgtgacgccg ggcggcatcc cggtgcgcaa cgtcgcgcag ccggtgctgg
     1981 gcgctgcgta cgcgacctac tgcctggagc cggcgccggt ccgcgccggc taagcgctct
     2041 tctccacgac ggctcccgcg cggtcacgca ggattcgagc cacgatggtc tcggcgcggc
     2101 tgaggccccg gatgcggcgc gttcgcctgc cccatgcctc catcagcgtt ccctcgcgat
     2161 aggcctcgag gatctcgggg tgcacgtacg acgtgcgcgc gatcgcgcgc gtgttgccga
     2221 gcgcgcctgc gacctcgtcg atcgcctcgt tgatcccgcg ggcggcctcg gcgtcggatg
     2281 ccggcgggtc cgcggccgcg agcacgatcg ccgccacgac cgtaccggcc caggtccgga
     2341 agtctttcgc ggtgaagtcg ctgccggtgg cttcgcgcag atactcgttg acgtcgccgg
     2401 agccgacggt gcggtccacg ccctcgtcgt cgaggtagcc gaacaaggtc tggcccggaa
     2461 gccgccggca ctttcggacg acgcgggcag cgcgcggatc cttcgcgtcg atctcgtgca
     2521 ccttgccgct cttgcccttg aatcggaaac gcatggcctc gccgtcaacg gtcacgtgct
     2581 tggcccgaag cgtcgtcaag ccgaacgagc cgttctcgcg ggcgtattcg tcgttgccga
     2641 cgcgcatcag cgtgcgctcg agcagccgca cgaccgcggc gaggacccgc tcgcgtggga
     2701 gcccgctgcg cgcgaggtcg cggtccacct tggcacgcag cttcggcagc gccgcggcga
     2761 actcgcgcaa gcgcgcgtat ttggtctcgt cacggatctc gcgccagcgc gggtgatagc
     2821 ggtactgctt gcggccgcgg gcgtcgcggc cggtggcctg cacgtggcct cgcccgtcgg
     2881 ggcagatcca cacgtcttcc catgccggcg gaatcgccag cgtccggatg cgctccagcg
     2941 tcgcgcggtc gcgaacgggc cggccaccgg cgcgcacgta gcgaaagcct tttccgctgc
     3001 gaacgcggcg gatgcccgga tcgtcgtcgc agacgtgacg aagccccgcc tcggcggcgt
     3061 cgcggcgcgc ggccgtgcgg cgtgagcgag tcgaggagcg ggacgcggtc ggagtggcca
     3121 cgcgagatgg ctacccgaag caagccgagc cgaaacgagg cggttgccgc ttggtttgtg
     3181 gcaatcctcc gcgggatgcg gatagccttg ccgccatgtt cggcgggggg aggattcgag
     3241 cggtcgcgct cctggtcgcc ctgacggcgc ttatcgcgcc cgaggcgcgc gcctccgacc
     3301 gcgcgtcgtt cccgctcccc cgcggctaca cgatcgccgg catcgaccat ctcgccgacg
     3361 gcgtgacgca cgtgcgctat gtggcgctga gcccacgacg aaacgtcaac gtcgcgttca
     3421 tccgtcccgg cgcggccgcg cggatgaagg tcgtcgtgtc gaacgaccgc atcgccgggg
     3481 gcaccgagac cgtgcgctcg atgtgcgcgc gagccggctg catcatgggg gtcaacgccg
     3541 atctgttctc gttcggctcc ggccagccgg ccggcggcat ggcgtcgcag cgtgagttct
     3601 tcaagacacc gcagtccttt cgtccgcacc tgatgcaagc gggcgacgga tcgacctcga
     3661 tcggaaacct caccactacc atccgcttct tcgccagcta tcccgagcgc gtcgagccct
     3721 tctacatcca cgggatcaac gtgccgcgcg gcagcggaca ggcggtgctc tacacgccgt
     3781 tgtgggcgcc gaccacgcag acggggtcgg gattcgagat cacgctcgct ccggtcgatg
     3841 cggaggccac gccgcgcttc ggcgagccgc tgcgcgtgcg catggtccag ggcttcggcg
     3901 ccggaagctc gtcgctgctg ccgggtacga tggtgctgag cgcgcacggc ccgtacgcga
     3961 ccctgctgtc gaacctgtgg aacgacgtgc tcgccggacg cgcgacgggt gacgccgaac
     4021 tgactttcgc cgcgccaccc gaggccgaca ccttcaccgg cggctggccg cagctgctgc
     4081 gcggcggaca ccaatcgttc accgaccgca catggtcgtt ctacagcgaa caagcgcgca
     4141 cgctcgcggg acgaacggcc gacggcaccg taatgctcgc cgtgatcgac ggcggccgcg
     4201 gctcctactg gacgggtgtt cgcttgctcg acgcgatgcg ctttatgcgt tccatggggg
     4261 cggtggacgc gctgaacatg gacggcggcg gctcctcgac gttcgtggtg cgcggccgcg
     4321 ccgtgaacca tccgctgcgc ggcgagcgag gcgtcgcctc ggccttggtt cttgtgcccg
     4381 cctagcggga cgaaccggta cgattcgcga atcgcgcttg cgcgcgcccc ggcacaaccg
     4441 catgatcgga ccggccatgc atcgggggac cctcatcctg ctcacgctcg cgctcgtcgc
     4501 gaccgcgtgc agcgctgcgc ccaccggaac cggccggccc gcgccgtcg
//
LOCUS       DATLFO010000028         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_30582, whole genome shotgun sequence.
ACCESSION   DATLFO010000028 DATLFO010000000
VERSION     DATLFO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30582"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VM841_00955"
     CDS             complement(<1..260)
                     /locus_tag="VM841_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016325959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HVL88781.1"
                     /translation="MARAIGRKRREDPLDRPDFTIITGISGAGRSEAAKVLEDLGYFV
                     IDNLPPALIGKMAELAGQPNAGVKHVALVVDVRGGQFFAELEA"
     gene            complement(268..2148)
                     /gene="uvrC"
                     /locus_tag="VM841_00960"
     CDS             complement(268..2148)
                     /gene="uvrC"
                     /locus_tag="VM841_00960"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HVL88782.1"
                     /translation="MPDQAGWRPKPDSIPEQPGCYLFKDRHGRVIYVGKAKSLRSRLS
                     SYFQNPAFLHPRTTSMVDTAASVEWIVVASDVESLHLEYTLIKKHRPRFNVRYRDDKS
                     YPYLAITMNEDYPRAMVMRGAKRKGVRYFGPFAHAYAIRETLDLLLRTFPVRTCSQGV
                     FDRCRRIDRPCLFYDIGKCAGPCVGHVDEQQHRTIATDLIEFMEGSFDPVLKRLEREM
                     LDASDRLEFERAARLRDQLASVRTAIERQVVVSGKQENLDVIALAEDDIEAAVQVFFV
                     RAGRLTGRKGFVVDKVEDLDAGALLATFIRDLYMDEEDIPREILVPCPPEDEPLLQQW
                     LKEKRGGPVGFRVPQRGDKKTLFETVKQNAHESFTQHKLRRRSDFAARAKQLKSLQDA
                     LGLDDAPLRIECFDISNTGPLEVVGSMVVFEDGLPKRSDYRRFKIRTVEGQDDFASMN
                     EVIGRRFARYLAERDAPHDEKPRKFAYPPNLIVIDGGKGQLGAALSALANLGIQEVAA
                     IGLAKRFEEVWLPEATDAIRIPRGSDALYLLQHIRDEAHRFAITYHRERRGKRMTVSE
                     LDRVPGIGPAKKKQLIKTFGSVRRILQASEDEVAAVAGPVAARRLIQVLRGLEPGKPG
                     KD"
BASE COUNT          338 a          717 c          790 g          388 t
ORIGIN      
        1 gcctcgagct cggcgaagaa ctggccgccc cggacgtcca cgacgagggc gacgtgcttg
       61 accccggcgt tgggctgccc ggccagctcg gccatcttgc cgatcagggc gggcggcagg
      121 ttgtcgatca cgaaataccc gagatcctcg agcaccttgg ccgcctcgga gcggccggcg
      181 cccgagatgc cggtgatgat ggtgaagtcg gggcgatcca gcgggtcctc ccgccgcttg
      241 cgtccgatcg ctctggccac tggctcgtca gtcctttccc ggcttgcccg gctccaggcc
      301 tcgcagcact tggatcagcc gccgcgcggc gacggggccg gccacggcgg cgacctcgtc
      361 ttccgatgcc tgaaggatac gccgcaccga tccgaaggtc ttgatgagct gcttcttctt
      421 ggccgggccg atgccgggca cgcgatcgag ctcgctgacg gtcatgcgct ttccgcgccg
      481 ctcccggtgg taggtgatcg cgaagcggtg ggcttcgtcg cggatgtgct gaagcaggta
      541 gagcgcgtcg ctgccgcgcg ggatccggat cgcatcggtg gcctcgggca gccagacctc
      601 ctcgaagcgc ttcgccaggc cgatcgcggc gacctcttgg atgcccaggt tcgcgagcgc
      661 gctcagggcg gcgccgagct ggcccttgcc cccgtcgatg acgatcagat tggggggata
      721 ggcgaacttg cgcggcttct cgtcgtgcgg ggcgtcccgc tcggcgagat agcgggcgaa
      781 gcggcgtccg atcacctcgt tcatcgaggc gaaatcgtcc tgcccttcaa cggtgcggat
      841 cttgaagcgg cgatagtcgc ttcgcttggg caggccgtcc tcgaacacga ccatcgaacc
      901 gacgacctcg agtggaccgg tgttggagat gtcgaagcac tcgatgcgca gcggggcgtc
      961 atccaggccg agcgcgtcct ggagggactt gagctgcttg gcccgggcgg cgaagtcgct
     1021 gcggcggcga agcttgtgct gcgtgaagga ctcatgcgcg ttctgcttga ccgtctcgaa
     1081 cagggtcttc ttgtcgcctc gctgaggaac gcgaaaaccg acagggccac cgcgcttctc
     1141 cttgagccac tgctgcaaaa gcggctcgtc ctcgggcggg cacgggacca ggatctcgcg
     1201 ggggatgtcc tcctcgtcca tgtagagatc gcggatgaag gtggccagca gcgcgcccgc
     1261 gtcgaggtcc tcgaccttgt ccacgacaaa gcccttgcgg ccggtcaggc gcccggcgcg
     1321 cacgaagaag acctgcacgg ccgcctcgat gtcgtcctcg gcgagcgcga tcacgtcgag
     1381 gttctcctgc ttgccgctga cgacgacttg gcgttcgatc gcggtgcgca cgctggcgag
     1441 ctggtcgcgc aggcgggcgg cccgctcgaa ctcgagccgg tcggaggcgt cgagcatctc
     1501 gcgttcgagc cgtttgagca ccggatcgaa cgagccttcc atgaactcga tcaggtcggt
     1561 cgcgatggtg cgatgctgct gctcgtcgac gtgtccgacg caggggccgg cgcatttgcc
     1621 gatgtcgtag aacaggcagg gccggtcgat ccggcggcac cggtcgaaca ccccttgcga
     1681 gcaagtacgc acgggaaagg tgcgcagcag cagatccagc gtctcgcgga tcgcgtaggc
     1741 gtgcgcgaac gggccgaagt accggacgcc cttgcgcttg gcgccgcgca tcaccatggc
     1801 acgcggatag tcctcgttca tcgtgatcgc caggtacgga tacgacttgt cgtcgcggta
     1861 gcggacgttg aagcgcggac ggtgcttctt gatcagggtg tactcgagat gcagcgactc
     1921 gacgtcgctc gcgaccacga tccattcgac cgaggcggcg gtgtccacca tgctggtggt
     1981 ccgggggtgc aggaacgccg gattctggaa gtagctggac aggcggctgc ggaggctttt
     2041 ggccttgccg acgtagatga cccggccgtg gcggtccttg aacaggtagc agcccggctg
     2101 ctcggggatc gagtcgggct tgggacgcca tccggcctgg tcgggcacgg gttcattgta
     2161 aggacgggag cgccggccgc cggacccgtt tcccgcggac ccggcagagg gtctcccgga
     2221 ccctgtgcaa cca
//
LOCUS       DATLFO010000029         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_109892, whole genome shotgun sequence.
ACCESSION   DATLFO010000029 DATLFO010000000
VERSION     DATLFO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109892"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VM841_00965"
     CDS             <1..267
                     /locus_tag="VM841_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL88783.1"
                     /translation="APDIVKLDTALTREVERDVARHAMVAGLVTFADRVDARLVAEGI
                     ETPEARDILRSLGVRFGQGYLFARPRPAREVTRDLAEHGWRIAS"
     gene            complement(376..449)
                     /locus_tag="VM841_00970"
     tRNA            complement(376..449)
                     /locus_tag="VM841_00970"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Ile,seq:cat)
     gene            complement(626..2749)
                     /locus_tag="VM841_00975"
     CDS             complement(626..2749)
                     /locus_tag="VM841_00975"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HVL88784.1"
                     /translation="MRHRMLQAILAMLALVAAFPTAHATTDPTVITVLSNRADLISGG
                     DALVEISPALQPGSVVTVDGDDVTNVFSATGSTSRGLVTGLAVGANQLTVTHADASGA
                     TLTIVNHPVEGPIFSGPHLQPWDCTTAANGLGAPKDAACNADPIVDYLYVSTAGGAYQ
                     AYDVNNPPLDVAMVTNDRGATVRNIVRRERGAINRGLYAIAYLHDPALGQPDPRAPQA
                     GFNGKLYYPFGASCNTNQTQGSMPGVINQTRLQEGWAIATGSLNVLGHHCNPVLSAET
                     VMMIKERVIEQLGSIRYTMATGGSGGAIGQLQVSNAYPGLVNGLMPGSMFPDVWSTAI
                     EVADCVLTETYFPVAAIPFTPMQKAAVDGHGPPQSTCATWVGTFAPSGIPTTGCFSNS
                     TIPTTSVPEPPVLSGRDYFPILHADGCRATVQDIQVNVWGRRPDGFAKRPLDNVGVQY
                     GLEALNLPVGNPGKITFAQFLDMNARIGGVDIDGVKQVDRTRTDDTVPRDVYRTSMIN
                     DGRGLARTAIINFAASQNIEIHTPYHAYALEARMAKIGHADNHVIWHNGPGELAWRTM
                     DAWLAGVEADGGIDPLTGMDPQLVKNNRPTIAHDSCWSGSAQQPLSACASQVFGDARL
                     KAGMPLSHDNLKCVLKPIDPADYAGAFPALTASDLAALELVFPEGVCNYAVEGVDKAP
                     SVPWLSYASGPGGQPLGLPPASTQF"
     gene            2944..>3129
                     /locus_tag="VM841_00980"
     CDS             2944..>3129
                     /locus_tag="VM841_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL88785.1"
                     /translation="MRPHHPLIGRRSDRERLATFVDTLAGGTGAFVLVEGEAGIGKSA
                     VLAEVVERALDRGMTAFI"
BASE COUNT          492 a         1044 c         1072 g          521 t
ORIGIN      
        1 gcgccagaca tcgtaaagct ggataccgcc ctcacgagag aggtcgagcg ggacgtcgca
       61 cgccacgcaa tggtcgccgg actcgtcacg ttcgccgatc gtgtcgatgc gcgcctagtt
      121 gccgaaggta tcgagacgcc agaagcgaga gacatcctac ggtccctcgg cgtgcgcttt
      181 ggtcagggat accttttcgc ccggcctcgg cccgcgcgcg aggtgacgcg ggatctggcc
      241 gagcatgggt ggcgaatcgc aagctagaga acatcgggcc gaggtcggga ctgcccgctg
      301 aatgctttgc catcagtgtg ccgtcaactc acccactccg acgacaaccg acacacaaaa
      361 ccccaggtga acgagtgggc ccgccagggc tcgaacctgg aaccgacgga ttatgagtcc
      421 ggcgctctaa ccacttgagc tacgggcccg cggacgtgag catatccggc cgttcgccgg
      481 caaggccgcc gcgttcgccg gcaaggccgc cgcgttcgcc ggcgttcgac ggtcgcgtgc
      541 ccgcggggct tcttcccacc gccgcgcagg cgcaccggac gcgaacggga ggcgcccgcg
      601 gtgggcgcct cccgttcgtt ccggtctaga actgcgtgga tgccggcggc aggccgagcg
      661 gctgcccgcc cggtcccgac gcgtagctga gccacggcac cgacggtgcc ttgtccacgc
      721 cctcgacggc gtagttgcac acgccctcgg ggaacacgag ctcgagcgcg gccagatcgc
      781 tggccgtgag cgcggggaac gcgcccgcgt aatcggccgg gtcgatcggc ttgagcacgc
      841 acttgaggtt atcgtgcgac agcggcattc cggccttgag tcgcgcgtcg ccgaacacct
      901 gcgatgcgca agcgctcagc ggctgctggg cgctgccgct ccagcacgag tcgtgcgcga
      961 tcgtgggccg gttgttcttg acgagctgcg gatccatgcc ggtgagcgga tcgatgccgc
     1021 cgtcggcttc gacgcccgcc agccacgcgt ccatcgtgcg ccaggcgagc tcgcccgggc
     1081 cgttgtgcca gatcacgtgg ttgtcggcgt ggccgatctt ggccatgcgg gcctccagcg
     1141 cgtaggcatg gtacggcgtg tggatctcga tgttctgact ggccgcgaag ttgatgatcg
     1201 cggtgcgcgc gagcccgcgg ccgtcgttga tcatgctcgt tcgatacacg tcccgcggga
     1261 cggtatcgtc ggtgcgggtg cgatccacct gcttgacccc gtcgatgtcc acaccaccga
     1321 tgcgcgcgtt catgtcgagg aactgcgcaa acgtaatctt gcccgggttt ccgaccggca
     1381 ggttgagcgc ttccaggccg tactgcaccc cgacgttatc gagcggccgc ttcgcgaagc
     1441 cgtccggccg tcgtccccag acgttcacct ggatgtcctg cacggtcgcg cggcagccgt
     1501 ccgcgtgaag gatcgggaag tagtcgcggc cgctgagcac cggcggctcc ggcaccgacg
     1561 tcgtcgggat cgtggagttg ctgaagcagc cggtcgtcgg gatgccgctg ggagcgaacg
     1621 tgccgaccca ggtcgcgcag gtggactgcg gcgggccgtg cccgtcgacg gcggccttct
     1681 gcatcggcgt gaacgggatc gccgcgaccg ggaagtaggt ctcggtcagc acgcagtcgg
     1741 caacctcgat cgcggtgctc cacacgtcgg ggaacatcga gccgggcatc aggccgttga
     1801 ccagacccgg gtaggcattg ctgacctgaa gctggccgat cgcgccgccc gagccgcccg
     1861 tggccatcgt gtagcggatc gaaccgagct gctcgatgac gcgttctttg atcatcatca
     1921 cggtctcggc cgacaggacc gggttgcagt gatggccgag cacgttcagc gagccggttg
     1981 cgatcgccca cccttcttga aggcgcgtct ggttgatcac gccgggcatc gagccctgcg
     2041 tctggttggt attgcagctc gcgccgaacg ggtagtagag cttgccgttg aagccggcct
     2101 gcggcgcgcg cggatcgggc tggcccaacg ccggatcgtg caggtaggcg atcgcgtaca
     2161 gaccgcggtt gatcgcacca cgctcgcggc gaacgatgtt gcgcacggtt gcgcctcggt
     2221 cgttggtcac catcgcgacg tccaacggcg gattgttcac gtcgtaggcc tgataggcgc
     2281 cgccggcggt agagacgtac agatagtcca cgatcggatc ggcgttgcat gccgcatcct
     2341 tgggcgcacc caggccgttg gcggcggtgg tgcaatccca cggctgcagg tgcgggccgc
     2401 tgaagatcgg cccctcgacc gggtggttga cgatcgtcag cgtcgcaccc gacgcgtcgg
     2461 cgtgcgtgac ggtcagctgg ttcgccccga ccgccaggcc ggtgaccagc ccgcgcgagg
     2521 tgctgccggt cgcggagaac acgttcgtca catcgtcgcc gtccacggtc acgacgctcc
     2581 ccggctgcag ggccggcgag atctcaacga gcgcgtcacc ccccgagatc agatccgccc
     2641 tgttggaaag caccgtgatc acggtggggt cggttgtcgc gtgcgcggtg gggaaggcgg
     2701 cgaccaaagc cagcatggcc agaatcgcct gcagcatccg gtgtctcatt gtgcgacctc
     2761 ctcgacgtcg tcgatcgagt gatcgagcca caaaggcccg cggtcaccgc cacgccggcg
     2821 cgaccaacaa cgggtattcg gcgacatcgg ccgagactcc tcctcgagat cagatctctc
     2881 cgtaacgact tcggccggac tccacctaga atcacgttga tcctcggcat cgggaggagt
     2941 tccatgcgtc cccatcaccc gctcatcgga cggcgcagcg acagggagcg gctcgccacg
     3001 ttcgtcgata cgctcgccgg cggcacgggt gcattcgtgc tggtcgaagg cgaggccggc
     3061 attggcaaga gcgccgtgct cgccgaggtc gtcgaacgcg ccctcgaccg gggcatgacc
     3121 gcattcatc
//
LOCUS       DATLFO010000030         6501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590 k141_568,
            whole genome shotgun sequence.
ACCESSION   DATLFO010000030 DATLFO010000000
VERSION     DATLFO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VM841_00985"
     CDS             <1..1153
                     /locus_tag="VM841_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL88786.1"
                     /translation="PMHLRVISYLPLAHVAERAVTHYAGLHRANDMYFVPELTQAVEV
                     AKEVHPDIFLGVPRVWEKAKAAIEAKMRDESSGAKKAIAAAAVKAGRKAAALEGAGKP
                     VPPLLAFKRGLFDKLVFSKIRHGLGLDQVKVVLTGSAPIAPDVIEFFHAAGLKILDTW
                     GMTELTVVASINPMDSPRIGTVGVPIPGVEIKIAEDGEILARGGIVTGGYRNRPKETA
                     ETFVDGWVHSGDLGMIGSDGHLRITGRKKELIITAGGKNISPNNIEDLLKAHPLVGQV
                     CVVGDAKPYLTALVAIDGETAPAWAAENGVPFTDVASFSRDDRVRAEIQRAVDQVNQR
                     VAKVESIKKFVLLPVEWTPDTGELTPTLKLKRSVVHERYADEIAGLYSG"
     gene            complement(1170..1241)
                     /locus_tag="VM841_00990"
     tRNA            complement(1170..1241)
                     /locus_tag="VM841_00990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Arg,seq:ccg)
     gene            1356..1748
                     /locus_tag="VM841_00995"
     CDS             1356..1748
                     /locus_tag="VM841_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL88787.1"
                     /translation="MAKKVLVVDDDRVIQQLLVVNLELEGYEVDTASDGEQALAKIAS
                     VDPDIVLLDIMMPKYDGWEVCRRAKADPTTAHIPIIFLSARAQDLDVRRGYEIGVAAY
                     MTKPFDPLELMDVVKRVLAGERILPADS"
     gene            1757..3433
                     /gene="argS"
                     /locus_tag="VM841_01000"
     CDS             1757..3433
                     /gene="argS"
                     /locus_tag="VM841_01000"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HVL88788.1"
                     /translation="MMRDRLTAAVRAAALAVAGDALPAGLPGFTMERPARKEHGDWSA
                     NVAMMLAKPLGRPPRAIAQQIVDALEPLPEVERIEIAGPGFINFALRADSLTEVVREI
                     LGAGQRWGRHREPDGERINVEFVSANPTGPLHVGHARWAALGDALSRILDAAGHTIIR
                     EFYVNDYGRQMDLFGASIAARYLGLFSRDAEIPEGGYGGVYVTELAEQIKAADGDRYL
                     EMDDAERAKAFRELGRERMLAHQRETLETFRVSFDVWFSETSLHESGAVERALDRLRD
                     GGHGYEQDGALWLRSTDFGDDKDRVARRGDGTTTYLAADIAYYLDKRSRDFDRIIFLW
                     GADHHGYVPRMKAVAQAVGDDVETVEFLIGQMVNLLRGGEPVRMSKRTGELVTAGELI
                     DEVGADAARYTLVRQSADTPIDFDIEMVVRQSQDNPVFYVQYAHARIASIGRNAAEQG
                     IARGDLAGAGLSLLTHDSEHDLLRKIGEFPEIVELAARRRAPHLIPRYAEELAGTFHS
                     FYRDCRVLTDDAALTQARLHLCEATRITLANALSLLGVSAPDRMERTEEI"
     gene            3433..4737
                     /gene="lysA"
                     /locus_tag="VM841_01005"
     CDS             3433..4737
                     /gene="lysA"
                     /locus_tag="VM841_01005"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVL88789.1"
                     /translation="MTHTLKDVLPHSALVAEDGHLHIGGVDVVELAAEHGTPLYVLCE
                     RTLRERCTTYRESLPGASIYYASKAFLCTEVARIVAEEGLNLDVASGGELFTALRAGV
                     DGERLIMHGNNKSDAELRMAVENGVGRIVADSIEELERIDATAGALSTRQRVLLRVTP
                     GVEAHTHEYVQTGQEDSKFGMSIEGGVALEAVKRAKDLDRIHVGGFHCHIGSNITGVE
                     AFLKTVEIMGEFLGEVRDETGIEAEELNLGGGLGIAYTREDPPARVELFAREVAAAVE
                     REALKHSLPQPKLSVEPGRWLVANAMVTIYSVGTVKEIPGVRTYVAVDGGMSDNIRPA
                     LYGARYEAFLANKANEQAGRVVTIAGAHCEQGDLLIRDIALPESVQRGDLLAIPATGA
                     YGYSMASNYNKFLRPPVVAVMNGAARVIVRRETYEDLVRLDA"
     gene            complement(4734..5690)
                     /locus_tag="VM841_01010"
     CDS             complement(4734..5690)
                     /locus_tag="VM841_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88790.1"
                     /translation="MDSFADAQARIFDLYNEGNFEEALEVATAAQSHFPTERNALVYY
                     RVCLAARTGQPSLATWILEEAMSTGIWFGVEKLRTERDFESLQGDATFERLVTINAER
                     QTEAARHTAPALMTVSPDGPKPPWPVVLALHGGSGTASGALPRWIAARRAGWLLALPQ
                     SSRPSSLGRYTWEAHDASAEARVADEILSHTETLARLGTTDPDRLVLGGSSAGGATSL
                     RLALTGTIAARGVVVVVPAVSRVEAFDPHLEGAAKRGLRVAIVAGGRDSDRSEPIRRM
                     HAMMEQAGIASRLHVDPAVAHDFPRDFDNLLPELLDWTLAAG"
     gene            5747..>6501
                     /locus_tag="VM841_01015"
     CDS             5747..>6501
                     /locus_tag="VM841_01015"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVL88791.1"
                     /translation="MSERVVRIGQLGCGIVGSGVLRMLHANGADIEERIGARLEVAAV
                     AVRSASKKRDIPEAMQPLLGADAQAIVDDPSIDVVVEVMGGIEPARSLILAALRNGKR
                     VVTANKELVASLGKELFDAADAAGMDLRFEGAVGGGIPLIRPIKEGLAGDKVRKVMGI
                     VNGTTNFILTRMSEEGLEFGEALAEAERLGYTELDPSADIEGFDPAAKCAILASIAFN
                     SRVVAGDVFREGIANVTATDIAFAKHVAGNHARV"
BASE COUNT         1067 a         2224 c         2227 g          983 t
ORIGIN      
        1 gccgatgcat ctgcgggtga tctcgtacct gccgctcgcg cacgtcgccg agcgtgccgt
       61 cacccactac gcgggcctgc accgcgccaa cgacatgtac ttcgtccccg agctgaccca
      121 ggccgtcgag gtggccaaag aagtgcatcc cgacatcttc ttgggcgtgc cgcgcgtgtg
      181 ggagaaggcc aaggccgcga tcgaagcgaa gatgcgcgac gagtcgtccg gggccaagaa
      241 ggcgatcgcc gccgccgccg tgaaagccgg ccgcaaagct gccgcgttgg aaggcgccgg
      301 caagccggtg ccgccgctgc tggccttcaa gcgcggcctg ttcgacaagc tggtgttctc
      361 gaagattcgt cacgggctcg gattggatca ggtcaaggtc gtgctgaccg gcagcgcgcc
      421 gatcgcgccg gacgtgatcg agttcttcca cgccgccggg ctcaagatcc tcgacacctg
      481 gggcatgacc gagctgaccg tggtcgcctc gatcaatccg atggattccc cgcgcatcgg
      541 caccgtcggc gtgccgattc ccggtgtgga gataaagatc gccgaagacg gcgagatcct
      601 ggcgcgcggc ggcatcgtca cgggcggcta ccgcaaccgg ccgaaagaga ccgctgagac
      661 gttcgtggac ggctgggtgc actcagggga cctgggcatg atcggctccg acggtcatct
      721 gcgcatcacc ggccgcaaga aagagctgat catcaccgcg ggcggcaaga acatctcgcc
      781 gaacaacatc gaggatcttc tgaaggccca cccgctggtc ggccaggttt gcgtcgtcgg
      841 cgacgccaag ccgtatttga ccgcgctggt ggcgatcgac ggcgagaccg cgccggcctg
      901 ggcggccgag aacggcgtgc cgttcaccga cgtcgcgtcg ttctcgcgcg acgaccgcgt
      961 ccgcgccgag atccagcgcg ccgtcgatca ggtcaaccag cgcgtcgcca aggtcgagtc
     1021 catcaagaag ttcgtcctgc tccccgtcga atggaccccg gacaccggcg agctgacccc
     1081 caccctgaag ctgaagcgca gcgtcgtgca cgaacgctac gccgacgaga tcgcgggact
     1141 gtattcgggc tgattccgaa cgcagaaagt gcccccggca ggactcgaac ctgcgcaccc
     1201 ggctccggag gccggtactc tatcctctga gctacggggg cgagacgcgg aatcctagca
     1261 ggacggcggc cccgagaacg ggaaccgaac ggttatcgaa tcgagcggcg cggcctgcgg
     1321 cgatatgatg cgcgggttcc cgatgcggag gcgccgtggc gaagaaagtc ctggtcgtcg
     1381 atgacgaccg cgtgatccag cagctgctgg tcgtcaacct tgaattggaa ggctacgagg
     1441 tggatacggc ctccgacggc gagcaggcgc tcgccaagat cgcctcggtc gatcccgaca
     1501 tcgtgctgct ggacatcatg atgcccaagt acgacggctg ggaggtctgc cgccgcgcaa
     1561 aggccgatcc gaccacggcc catatcccga tcatcttttt gtcggcccgc gcgcaggacc
     1621 tggacgtgcg gcgcggctac gagatcgggg tcgcggccta catgacgaag ccgttcgatc
     1681 cgctcgagct gatggacgtg gtcaagcgcg tcctggccgg cgagcggatc ttgccggcgg
     1741 attcgtgagc cgggcgatga tgcgggatcg cctcaccgcc gcggtgcgcg cggccgcgct
     1801 ggccgtcgcc ggcgacgcgc tgcccgcggg cctgcccggc ttcaccatgg agcggcccgc
     1861 ccgtaaagag cacggcgact ggtcggccaa cgtcgcgatg atgctcgcca agccgctggg
     1921 ccgtccgccg cgcgcgatcg cgcagcagat cgtggacgcg cttgagccgc tgcccgaggt
     1981 cgagcggatc gagatcgccg gtcccggctt catcaacttc gcattgcgtg ccgattcgct
     2041 caccgaagtc gttcgcgaga tcctgggcgc cggccaacgg tggggccgtc accgcgaacc
     2101 ggacggcgaa cgcatcaatg tcgagttcgt ctcggccaat ccgaccgggc ccctgcacgt
     2161 cgggcacgcg cgctgggcgg cgctcggcga cgcgctctcg cgaatcctgg acgcggcggg
     2221 gcacaccatc atccgcgagt tctacgtcaa cgactacggc cggcagatgg atctgttcgg
     2281 cgcgtcgatt gccgcgcgct atctgggatt gttctcccgc gacgccgaga tccccgaagg
     2341 cggttacggc ggcgtctacg ttaccgagct ggccgagcag atcaaagccg ccgacggcga
     2401 tcgctacctg gagatggacg atgccgagcg cgcgaaagcg ttccgcgagc tcggccgcga
     2461 gcgcatgctc gcgcatcagc gcgagacgct ggaaacgttc cgcgtcagct tcgacgtctg
     2521 gttcagcgag acgagtctgc acgagtcggg cgcggtggag cgcgccttgg accggctgcg
     2581 cgacggcggc cacggctacg agcaggacgg cgcgctgtgg ctccgctcga ccgacttcgg
     2641 cgacgacaag gaccgagtcg cgcggcgtgg cgacggcacg acgacgtacc tggcagccga
     2701 catcgcgtac taccttgaca agcggtcgcg cgacttcgac cggatcatct tcctgtgggg
     2761 agccgatcat cacggctacg tgccgcgcat gaaggcggtc gcgcaggcgg tcggcgacga
     2821 tgtcgagacg gtcgagttcc tgatcggcca gatggtgaac cttcttcgtg gtggcgagcc
     2881 ggtccgaatg tccaaacgca ccggcgaact ggtcaccgcc ggcgagctga tcgacgaggt
     2941 cggcgccgac gcggcgcgct acacgctggt gcggcaaagc gccgacaccc cgatcgactt
     3001 cgacatcgag atggtcgttc gccagtcgca ggacaatccg gttttctatg tgcaatacgc
     3061 gcacgcgcgc atcgcatcga tcggccgcaa cgccgccgag caaggcatcg cgcgcggcga
     3121 cctcgccggt gccggcctgt cgctgctgac ccacgattcc gagcacgacc tgctccgcaa
     3181 gatcggcgag ttccccgaga tcgtcgagct ggccgcccgt cgccgcgccc cgcacctgat
     3241 cccgcggtat gccgaggagc tggccggaac gtttcactcg ttctaccgag actgccgcgt
     3301 gctcaccgac gacgcggcgc tgacgcaggc tcggctgcac ctgtgcgagg cgacccggat
     3361 cacgcttgcg aacgcgctgt cgctgctggg cgtgtcggcg cccgaccgca tggaacgaac
     3421 cgaggagatc tgatgacgca cacgctcaag gacgtcctgc cgcattcggc cctcgtggcc
     3481 gaagacgggc atctgcacat cggcggcgtg gatgtcgtcg agctcgcggc cgagcacggc
     3541 acgcccctgt acgtcttgtg tgaacgaacg ctgcgcgagc gatgcaccac ctatcgcgag
     3601 tcgctgcccg gcgcctcgat ttactacgcg tcgaaggcgt tcctgtgcac cgaggtcgcg
     3661 cgtatcgtcg ccgaggaagg cctgaacctc gacgtcgcca gcggcggcga gctgttcacc
     3721 gcgctgcgcg ccggcgtgga cggcgagcgg ctgatcatgc acggcaacaa caagtcggat
     3781 gccgagctcc gcatggccgt cgagaacggc gtcggccgga tcgtcgccga ctcgatcgag
     3841 gagctcgagc gcatcgacgc gaccgccggc gcgctgagca cgcgccagcg cgtgctgctg
     3901 cgcgtcaccc ccggcgtcga ggcccatacg cacgagtacg tgcagaccgg ccaggaggac
     3961 tccaagttcg gcatgtcgat cgaaggcggc gtcgcgctcg aagccgtcaa acgcgccaag
     4021 gacctcgacc gcatccatgt cggaggcttc cactgccaca tcggctccaa catcaccggc
     4081 gtcgaggcgt tcctcaagac cgtcgagatc atgggcgagt tcctgggcga ggtgcgcgac
     4141 gagaccggca tcgaagccga agagctcaac ctcggcggcg ggctgggcat cgcctatacc
     4201 cgcgaggacc cgcccgcgcg cgtggagctg ttcgcccgcg aggttgccgc ggccgtcgag
     4261 cgggaagcgc tcaagcactc gctgccgcag cccaagctgt cggtcgagcc cggccgctgg
     4321 ctggtcgcga acgcgatggt caccatctac tcggtcggca ccgtcaagga gattcccggc
     4381 gttcgcacct acgtcgcggt agacggcggc atgagcgata acatccggcc cgcgctttac
     4441 ggcgcccgct acgaagcgtt ccttgccaac aaggccaacg agcaggccgg ccgcgtcgtg
     4501 acgatcgcgg gcgcgcactg cgagcagggt gatctgttga tccgcgacat tgcgctcccc
     4561 gagtcggtgc agcgcggcga tctgctggcg atcccggcca cgggcgcgta cgggtactcg
     4621 atggccagca actacaacaa gtttctgcgc cccccggtcg ttgccgtcat gaacggcgcg
     4681 gcgcgcgtga tcgtgcgccg cgagacctac gaagacctgg ttcgcctgga cgcctaaccg
     4741 gccgccagcg tccagtccag cagttcggga agcaggttgt cgaagtcgcg cgggaagtcg
     4801 tgcgcgacgg cgggatcgac atgcagccgc gacgcgatgc cggcctgctc catcatcgcg
     4861 tgcatccgcc ggatcggctc gctgcgatcg ctgtcccgtc cgcccgccac gatcgccacg
     4921 cgcaggccgc gtttggccgc accctccagg tgcgggtcga acgcctcgac gcgcgagacc
     4981 gccgggacga cgacgacgac gccgcgcgcc gcgatcgtgc cggtgagcgc caggcgcagc
     5041 gaggtcgcgc cgcccgccga cgatccgccc agcaccagcc ggtccggatc ggtcgtgccc
     5101 agccgcgcga gcgtttcggt gtgcgacagg atctcgtcgg cgacgcgcgc ttcggcggac
     5161 gcgtcgtgcg cctcccaggt gtagcgaccc agcgatgacg gccgcgacga ctgcggcagc
     5221 gccagcagcc atccggcgcg ccgcgccgcg atccatcggg gcagcgcgcc gctggcggtg
     5281 ccgctgccgc cgtgcagcgc cagcacgacc ggccagggtg gcttcggccc gtcgggcgag
     5341 acggtcatca gcgcgggcgc ggtgtgacgc gcggcttcgg tctggcgttc ggcgttgatc
     5401 gtcaccagcc gttcgaaggt ggcgtcgccc tgaagcgatt cgaagtctcg ttccgtgcga
     5461 agtttctcga ccccgaacca gatgccggtg gacatggctt cttccaggat ccaggtcgcc
     5521 agcgacggct ggccggtccg cgccgccagg cagacgcggt agtagaccag cgcgttgcgt
     5581 tcggtcggga aatggctttg cgcggcggtt gcgacttcca gggcttcctc gaagttgccc
     5641 tcgttgtaca ggtcgaagat gcgcgcttgg gcgtccgcga agctgtccat tcatcaagcc
     5701 tacccaacgg aaccggctcc gggcccgccc ggtatagttc tgcgccatga gcgaacgagt
     5761 ggtccgcatc ggccagctgg gctgcggcat cgtcggttcg ggcgtgctgc gcatgctcca
     5821 cgccaacggc gccgacatcg aggagcgcat cggcgcgcgg ctcgaggtcg cggcggtcgc
     5881 ggttcggtcg gcctcgaaga agcgcgacat tcccgaggcg atgcagcccc tgctgggtgc
     5941 cgacgcacag gcgatcgtgg acgatccgtc catcgacgtc gtcgtcgagg tcatgggcgg
     6001 tatcgagccg gcccgctcgc ttatcctggc ggccctgcga aacggcaaac gcgtcgtgac
     6061 cgccaacaag gagctcgtcg cctcgctcgg caaggagctg ttcgacgccg ccgacgcggc
     6121 cggcatggac ctgcgcttcg aaggcgcggt cggcggcggc attccgctga tccggccgat
     6181 caaggaaggc ctggccggcg acaaggtccg caaggtcatg ggcatcgtca acggcacgac
     6241 caacttcatc ctgacgcgga tgagcgagga gggcctcgag ttcggcgagg cactcgccga
     6301 ggccgagcgg ctcggctaca ccgagctcga cccgagcgcc gacatcgaag gcttcgaccc
     6361 ggcggccaag tgcgcgatcc tcgcctcgat cgcgttcaac tcgcgcgtgg ttgccggcga
     6421 cgtgtttcgc gaaggcatcg ccaacgtcac cgcgaccgac atcgccttcg cgaaacacgt
     6481 cgccggcaac cacgcgcgag t
//
LOCUS       DATLFO010000031         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_21045, whole genome shotgun sequence.
ACCESSION   DATLFO010000031 DATLFO010000000
VERSION     DATLFO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21045"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..189
                     /locus_tag="VM841_01020"
     CDS             31..189
                     /locus_tag="VM841_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88792.1"
                     /translation="MGLVLATTFGLVVWIVLWAIGWKSIDAFIVTTVIITLGATAKLL
                     FPYLPNRH"
     gene            198..1433
                     /locus_tag="VM841_01025"
     CDS             198..1433
                     /locus_tag="VM841_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL88793.1"
                     /translation="MSRGLRAAAVALSAFVLASGPAACGSENKSAVPAGSDTLTIYSS
                     LPLHGRDRERSRDMVNAIKLALQEAGGKVGPFTITYVPLDAATPEAGTWTAEKVLDNA
                     RKAIRDINAIAYIGERDSAATALSLPLTNEGHILQVSPSSTYDGLTRASRRQGEPERF
                     YPSGERTFARVVPADHVQASALVGYMKESGVRSLAMLADREIYGGGLADQVAKAAERQ
                     GIEVFDRGRINASRRDVAGAAQNVAETAADAFLFAGTTDTGAARIFTAVAEAAPSMLL
                     FGPAAVADRSFLQELPVAVRRRMRVTTPSLPPRLLPPSAAAFRDSFRRTFGRPPAPEA
                     LQAYEAAKAVLHAIGSAGTRGNSRQAVTDAFFAIRDRESVLGRYSIDANGDTSLSTYA
                     GNRVRNAQFVLDKVLEVRR"
     gene            1536..>2226
                     /locus_tag="VM841_01030"
     CDS             1536..>2226
                     /locus_tag="VM841_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL88794.1"
                     /translation="MKLHRVVAVSSVLVLSAFGVAACGDDDDDDAGAGAGATTQAAGG
                     SLTIYSSLPLQGASRTQTTAMVSGIRLALKEVNNKAGNFTIRYQSLDDSTAQQGGWTP
                     EAVSQNARKAAQDDKTAVYIGEFNSGASAVSIPILSDAKVPQISPANTAVGLTSDEPG
                     ADKGEPDKYYPSGFRNYVRIVPKDTIQGAALATIMKEDGCTKVAMANDKEVYGAGLAR
                     NIAMSAKEQDLQ"
BASE COUNT          356 a          822 c          735 g          313 t
ORIGIN      
        1 gccgcgctca tcggttagtc tggcgccgca atgggtctcg tcctcgccac gaccttcggt
       61 ctcgtcgtat ggatcgtcct gtgggcgatc ggctggaagt cgatcgacgc attcatcgtc
      121 acgaccgtga tcatcacgct cggcgccacc gcgaagctcc tcttcccgta cctgcccaac
      181 cggcactagc cgcgccggtg tcccgcggtc ttcgcgcggc tgccgtcgcc ctgagcgcgt
      241 tcgtgctcgc atccgggccg gcggcctgcg gcagcgagaa caagagcgcc gtgcccgcgg
      301 gcagcgacac gctgacgatc tactccagcc tgccgctaca cggccgcgac cgcgagcggt
      361 cgcgcgacat ggtcaacgcg atcaagctcg cgctccagga ggcgggcggc aaggtcggcc
      421 cgttcacgat cacgtacgtg ccgctggacg cggctacgcc ggaggccggc acatggaccg
      481 ccgagaaggt gctcgacaac gctcgcaagg ccatccgcga catcaacgcg atcgcctaca
      541 taggcgagcg cgactcggcg gccaccgccc tctcgctgcc gctgacgaac gaaggccaca
      601 tcctccaggt cagcccgtcg agcacctacg acggcctcac ccgtgccagc cgccgccagg
      661 gcgagcccga gcgcttctac ccctccggcg agcggacgtt cgcccgcgtc gtgcccgccg
      721 accacgtcca ggcatcggcg ctggtcggct acatgaagga gtcgggcgtg cgcagcctcg
      781 cgatgctcgc cgaccgcgag atctacggcg gcggcctcgc cgaccaggtc gcgaaggccg
      841 ccgagcgcca ggggatcgag gtcttcgatc gcggccggat caacgccagc cgccgcgatg
      901 tcgcgggcgc ggcgcagaac gtcgccgaga cggccgccga tgcgttcctc ttcgccggga
      961 ccaccgacac gggcgccgcg cggatcttca ccgccgtcgc cgaggccgcg ccctcgatgc
     1021 tcctgttcgg gccggcggcc gtggccgacc gctcgttcct ccaggagctg ccggtggcgg
     1081 tgcggcgccg gatgcgcgtc acgacgccgt cgctgccccc gcgcctgctg ccgcccagcg
     1141 ccgcggcgtt ccgcgactcc ttccgcagga cgttcggccg cccgcccgcg cccgaggcgc
     1201 tgcaggcata cgaggcggcg aaagccgtgc tgcacgcgat cggctccgcc gggacgcggg
     1261 gcaacagccg gcaggccgtc accgacgcct tcttcgcgat ccgcgaccgc gagtcggtgc
     1321 tgggcaggta ttcgatcgac gccaacggcg acacgagcct gtcgacctat gccggaaatc
     1381 gcgtgcggaa cgcgcagttc gtcctcgaca aggtgctcga ggtccgtcgc tgaggcactg
     1441 catctccttg cacgccgtca tccactgcat accggtgcgg gtgaacgcgt gtacgatcgc
     1501 gcacaagttc gctttccagt ccccaggagg atcctttgaa actgcaccgg gttgtcgctg
     1561 tcagctctgt actggtccta tcggccttcg gcgtcgcggc ctgtggggac gatgacgacg
     1621 atgacgccgg cgccggcgcc ggcgccacga cgcaggccgc cggcggttcc ctgacgatct
     1681 actcgagcct gccgctgcag ggggcctcgc gcacgcagac gacggcgatg gtgagcggca
     1741 tccgccttgc gctgaaggaa gtcaacaaca aggccggcaa cttcaccatc aggtaccagt
     1801 cgctcgacga ctcgaccgcg cagcagggcg gctggacgcc ggaggccgtc tcccagaacg
     1861 cgcgcaaggc cgcccaggac gacaagaccg cggtctacat cggtgagttc aactccggtg
     1921 ccagcgccgt ctcgatcccg atcctcagcg acgcgaaggt cccgcagatc tcgcccgcga
     1981 acaccgcggt cggcctgacc tccgacgagc cgggcgcgga caagggcgag cccgataagt
     2041 actacccgtc gggcttccgc aactacgtcc gcatcgtgcc gaaggacacg atccagggcg
     2101 cggcactggc gacgatcatg aaggaggacg gctgcacgaa ggtcgccatg gccaacgaca
     2161 aggaggtcta cggcgccggc ctggcacgca acatcgccat gtccgccaag gagcaggacc
     2221 tccaga
//
LOCUS       DATLFO010000032         7208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_99940, whole genome shotgun sequence.
ACCESSION   DATLFO010000032 DATLFO010000000
VERSION     DATLFO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99940"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="rplC"
                     /locus_tag="VM841_01035"
     CDS             <1..238
                     /gene="rplC"
                     /locus_tag="VM841_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073036.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HVL88795.1"
                     /translation="RKHRSPGSQGAGTTPGRVFKGKKLAGRLGHERVTIMSLEVVETD
                     TDKGLLLIKGAIPGPKGSVVMIRSAVKAKAKVEQ"
     gene            235..912
                     /gene="rplD"
                     /locus_tag="VM841_01040"
     CDS             235..912
                     /gene="rplD"
                     /locus_tag="VM841_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HVL88796.1"
                     /translation="MSEVRVYSQSGEASGSVTLDESVFGSQVNVPVMHQVVVAQLAAA
                     RAGTHSTKHRGEVSGGGKKPWRQKGTGRARQGSTRAPHWTGGGVVFGPRPRDHSVKVP
                     KKMKALALRSALSDRAAAGTIAVIDKWEFSEPSTKAAKALLDKVGVKGSALLVFDAVD
                     EAAVKSFRNMKDCHLILASQLNTYDVLAADWIVFTKRALEQVTEARKAEPSRREKAAA
                     AKGGEKA"
     gene            909..1196
                     /gene="rplW"
                     /locus_tag="VM841_01045"
     CDS             909..1196
                     /gene="rplW"
                     /locus_tag="VM841_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HVL88797.1"
                     /translation="MKDPRDVILSPVVSEKSYSSIDQNVYTFIVDHRASKTEIKIALE
                     KIFGVKVTKVNTMWRRGKKKRTGMVTGTRSSHKRALVTLAEGDKIEIFQPR"
     gene            1199..2029
                     /gene="rplB"
                     /locus_tag="VM841_01050"
     CDS             1199..2029
                     /gene="rplB"
                     /locus_tag="VM841_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HVL88798.1"
                     /translation="MGVRRYKPTTPGRRGASVSDYSEITRSKPEKSLTEKLSSTGGRN
                     SNGRITSRHRGGGNKRRYRIVDFRRNKDGVPAKVFSIEYDPNRNCRIALLHYADGEKR
                     YILAPDKLEVGDKVGSGEGVDIKPGNALMLRNIPVGTVVHAVELRPGGGAKIARSAGT
                     SVQLLAKEGTMAHLRMPSGEVRLVSSQCRATVGEVGNSEWELINWGKAGRTRYKGFRP
                     KVRGVAMNPVDHPLGGGEGKTSGGRHPVSPWGKPEGRTRKQKASDKLIVRRRKTKKRG
                     "
     gene            2034..2321
                     /gene="rpsS"
                     /locus_tag="VM841_01055"
     CDS             2034..2321
                     /gene="rpsS"
                     /locus_tag="VM841_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HVL88799.1"
                     /translation="MARSLKKGPFVDDHLLEKVEEASRRKDKRVIKTWSRRSTIIPDM
                     VGLTIAVHDGRKHIPVYVSESMVGHKLGEFAPTRTFRTHGGSTKEKGTGAR"
     gene            2324..2677
                     /gene="rplV"
                     /locus_tag="VM841_01060"
     CDS             2324..2677
                     /gene="rplV"
                     /locus_tag="VM841_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HVL88800.1"
                     /translation="MATKTQSVTARVKYVRMTPNKVRFVLNLIRGKHVDEARRVLQFT
                     PKAACHEILKVLNAAVANAENTYNLDPDTLVVAACWADEGPTLKRWQPRALGRAYKVL
                     KRTSHITVVVEPREV"
     gene            2682..3770
                     /gene="rpsC"
                     /locus_tag="VM841_01065"
     CDS             2682..3770
                     /gene="rpsC"
                     /locus_tag="VM841_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HVL88801.1"
                     /translation="MGQKINPYGFRLGANPAFKWRSRWYADKQYLPWLQEDLRVRKYV
                     MKSLAHAGIANIDIERKGDRLQVDIWTARPGIVIGRRGAEVDRIRDDLRKLTGKENVQ
                     LNVNEIKQPELVAKLVAQGIGEQLTSRVSFRRAMRRGVQSTMRAGAKGIRVQVGGRLG
                     GAEMSRSEWYREGRVPLHTIRADVDYGLYEAHTTFGRIGVKVWIYRGDITAQQQEKEA
                     QIARERARAAAAGEIVAPARRKAGGRSAPRGVAQDLTAQRSAKPKDAPKDAPAGEGNP
                     KAEDKPKAGNKPKAEAQPAEAQAEDKPKAEDKPKAEAQPAEAKAETAPAAQESKSEAA
                     PAVETSAPDPAPGDAAAPNTSATEGTEA"
     gene            3774..4187
                     /gene="rplP"
                     /locus_tag="VM841_01070"
     CDS             3774..4187
                     /gene="rplP"
                     /locus_tag="VM841_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HVL88802.1"
                     /translation="MLAPKRVKHRKQHRGRMRGQAKGATTVHFGDFGLQALEPAWITN
                     RQIEAARVAMTRSIKRGGKVWVNIFPDKPFTKKPAETRMGSGKGNPEYWVAVVKPGRI
                     MFELAGVDESAAREALRLAAHKLPIKTRFVARQEG"
     gene            4191..4427
                     /gene="rpmC"
                     /locus_tag="VM841_01075"
     CDS             4191..4427
                     /gene="rpmC"
                     /locus_tag="VM841_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HVL88803.1"
                     /translation="MKASELKDMADDELATKLGEAKEELFNLRFQLVTGQLDNPMRLG
                     QVRKDIARIMTTIREREIAAAEAAGVGAEAGEAR"
     gene            4424..4681
                     /gene="rpsQ"
                     /locus_tag="VM841_01080"
     CDS             4424..4681
                     /gene="rpsQ"
                     /locus_tag="VM841_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HVL88804.1"
                     /translation="MNERGSRKTRVGVVVSDKMDKTVVVAVRSTIRHPLYGKIIRRVS
                     KVQAHDQDNSCGVGDRVLLAETRPLSKTKRWRVAEILEKAK"
     gene            4678..5046
                     /gene="rplN"
                     /locus_tag="VM841_01085"
     CDS             4678..5046
                     /gene="rplN"
                     /locus_tag="VM841_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HVL88805.1"
                     /translation="MIQQESRLKVADNSGAKEILCIRVLGGSGRRYAGIGDIIVASVK
                     DAIPGAGVKKGEVVKAVIVRTAKERRRPDGSSIRFDDNAAVIINEQQNPRGTRIFGPV
                     ARELRDKKFMKIISLAPEVL"
     gene            5049..5378
                     /gene="rplX"
                     /locus_tag="VM841_01090"
     CDS             5049..5378
                     /gene="rplX"
                     /locus_tag="VM841_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HVL88806.1"
                     /translation="MPGLKIRKDDNVEVITGKDKGKRGRVVRVFPDRGRVLVEHVNVV
                     KRHERLRVGKGRGGTEGGIITKEMPVDISNVLVVCPACDRGTRTGYLIEDTGAKRRVC
                     KKCGGDV"
     gene            5378..5929
                     /gene="rplE"
                     /locus_tag="VM841_01095"
     CDS             5378..5929
                     /gene="rplE"
                     /locus_tag="VM841_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HVL88807.1"
                     /translation="MATKTMPRLKERYRSEIVPHLMQERGYANIMMVPRLEKIVVNMG
                     LGEALKDAKILEGAVADLTVITGQKPLVTKARKSIANFKLREGQAIGAKVTLRGDRMW
                     EFADRLLTVALPRIRDFRGLPKKLDRRGNYTFGVTEQLIFPEVDYDKIYRTRGMDITF
                     VTTANDDDEARALLKALGVPFRE"
     gene            5933..6118
                     /locus_tag="VM841_01100"
     CDS             5933..6118
                     /locus_tag="VM841_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HVL88808.1"
                     /translation="MAKKCLIEKQRREPKFASRKYSRCQRCGRPRAYYRKFGLCRICL
                     RQLAHAGEVPGVTKSSW"
     gene            6126..6524
                     /gene="rpsH"
                     /locus_tag="VM841_01105"
     CDS             6126..6524
                     /gene="rpsH"
                     /locus_tag="VM841_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HVL88809.1"
                     /translation="MTMTDPIADMLSRIRNASSAHHDSVSIPSSKIKVGIADILKREG
                     YIEDFRIERGDKGPLLMVELKYGPNRERTILGIKRISKPGLRVFAQRDGIPRVLGGLG
                     IAIISTSKGLLTDKQAAKAGVGGEVVCYVW"
     gene            6532..7068
                     /gene="rplF"
                     /locus_tag="VM841_01110"
     CDS             6532..7068
                     /gene="rplF"
                     /locus_tag="VM841_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HVL88810.1"
                     /translation="MSRIGRKPIEIPSGVEIKLNGRHIVVKGPKGTLERDLPHDIVVK
                     KEDATLVVERPTDQRDHRALHGLTRSLVANMVTGVTEGFKKDLEIQGVGYRAAAKGKD
                     LEFALGFSHPVTVTAPEGISFEVPAPTKITVSGADKQVVGQVAAEIRALRPPDAYKGK
                     GIRYAGEYVRKKAGKAAK"
     gene            7071..>7208
                     /locus_tag="VM841_01115"
     CDS             7071..>7208
                     /locus_tag="VM841_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVL88811.1"
                     /translation="MDAKTKQVARLRRHHRVRKRVTGTQERPRLAVFRSNRHIYAQVI
                     DD"
BASE COUNT         1402 a         2273 c         2530 g         1003 t
ORIGIN      
        1 gcgcaagcac cgctcgcccg gatcgcaagg cgcgggtacc acgccgggtc gcgtgttcaa
       61 gggcaagaag ctggccggac gtctgggtca cgagcgcgtc acgatcatgt cgctggaggt
      121 cgtggagacc gacaccgaca agggtctgct gctgatcaag ggcgcgatcc cgggtccgaa
      181 gggctcggtc gtgatgatcc gctcggccgt caaggcgaag gcgaaggtgg agcagtgagc
      241 gaggtacggg tctattcgca gagcggcgag gcctcgggct cggtgacgct ggacgaaagc
      301 gttttcggct cccaggtcaa cgttccggtc atgcatcagg tcgtggtggc gcaactcgcg
      361 gccgcgcgcg ccggcacgca ctcgaccaag caccgcggcg aggtgtccgg cggcggcaag
      421 aagccgtggc gccagaaggg caccggccgt gcgcgtcagg gctcgacccg cgcgccgcac
      481 tggacgggcg gtggcgtggt tttcgggccg cgtccgcgcg accactcggt gaaggtgccc
      541 aagaagatga aggcgctcgc gctgcgctcg gcgctgtcgg accgcgccgc ggccggcacc
      601 atcgccgtga tcgacaagtg ggagttctcc gagccgagca cgaaggcggc aaaggcgctg
      661 ctggacaagg tcggcgtgaa gggctccgcc ctgctcgtgt tcgacgcggt ggacgaggcg
      721 gccgtgaaat ccttccgcaa catgaaggat tgccacctga tcctggcgtc gcagctcaac
      781 acctacgacg tgctcgccgc cgactggatc gtgttcacca agagggcgct cgagcaggtc
      841 accgaagccc gcaaggccga accgtcgcgg cgcgagaagg ccgctgcggc caagggaggc
      901 gagaaggcgt gaaggacccg cgtgacgtga tcctttcccc ggtcgtctcc gagaagtctt
      961 attcgtcgat cgaccagaac gtgtacacgt tcatcgtcga tcaccgcgcc agcaagaccg
     1021 agatcaagat cgcgctggag aagatctttg gcgtgaaggt cacgaaggtg aacacgatgt
     1081 ggcggcgcgg caagaagaag cgcaccggaa tggtgacggg cacccgctcg tcgcacaagc
     1141 gcgccctggt cacgttggcc gagggcgaca agatcgagat cttccagccg aggtaatcat
     1201 gggcgttcgc agatacaagc cgacgacacc gggccgccgc ggggcctcgg tctccgacta
     1261 ctcggagatc acccgcagca agcccgagaa gagcctgacc gagaagctgt cctccaccgg
     1321 aggccgcaac tcgaacgggc gcatcacgtc gcgtcaccgc ggcggcggca acaagcgccg
     1381 ctaccgcatc gtggacttcc gtcgcaacaa ggacggcgtg cccgcgaagg tcttctcgat
     1441 cgagtacgac ccgaaccgaa actgccgcat cgcgctgctg cactacgcgg acggcgagaa
     1501 gcgctacatc ctcgcgcccg acaagctcga ggtcggcgac aaggtcggct cgggtgaagg
     1561 cgtggacatc aagccgggca acgcgctgat gctgcgcaac atcccggtcg gtaccgtcgt
     1621 gcacgcggtc gagcttcgcc cgggaggcgg cgcgaagatc gcgcgcagcg cgggaacctc
     1681 ggtgcagctt ctggccaagg aaggcaccat ggcgcacttg cgcatgccgt ccggcgaggt
     1741 ccggctggtg tcgtcgcagt gccgcgccac ggtcggcgag gtcggcaact ccgaatggga
     1801 gctgatcaac tggggcaagg ccggacggac gcgttacaag ggcttccgcc cgaaggtacg
     1861 cggcgtggcc atgaacccgg tggatcaccc gctgggtggt ggcgaaggca agacgtccgg
     1921 cggacgtcac ccggtcagcc cctggggcaa gccggagggg cggactcgca agcagaaggc
     1981 gagcgacaag ctgatcgtgc gccgccgcaa gacgaagaag aggggctaac gggatggctc
     2041 gttcgctgaa aaagggtccg ttcgtcgacg atcatctgct cgagaaggtc gaggaggcgt
     2101 cgcggcgcaa ggacaagcgc gtgatcaaga cctggtcgcg acgctcgacg atcattcccg
     2161 acatggtcgg gctgacgatc gccgtgcacg acgggcgcaa gcacatcccg gtgtacgtga
     2221 gcgaatcgat ggtcggacac aagctcggtg agttcgcgcc gacgcgtacg ttccgtacgc
     2281 acggcggaag caccaaggaa aagggcaccg gggcgaggta ggtatggcga ccaagacgca
     2341 atccgtgacc gcgcgcgtca agtatgtgcg catgactccg aacaaggttc gcttcgtgct
     2401 gaacctgatc cggggcaagc acgtggacga ggcacgccgc gtcctgcagt tcacgccgaa
     2461 ggctgcctgc cacgagatcc tgaaagtctt gaacgccgcg gttgccaacg cggagaacac
     2521 ctacaacctc gatcccgaca cgctcgtcgt cgcggcatgc tgggccgatg agggaccgac
     2581 gctgaagcgg tggcagccgc gcgcgctcgg tcgcgcctac aaggtcctca agcgcacgtc
     2641 gcacatcacc gtcgtggtcg agccgaggga ggtttagtcg cgtgggtcag aagatcaatc
     2701 cctacggctt ccggctcggc gccaacccgg ccttcaagtg gcggtcgcgc tggtacgccg
     2761 acaagcagta tctgccgtgg ctccaggagg acctgcgcgt ccgcaagtac gtgatgaagt
     2821 cgctcgcgca cgccggaatc gccaacatcg acatcgagcg caagggtgac cgactgcagg
     2881 tcgacatctg gaccgcgcgg cccggcatcg tgatcggccg ccgcggcgcc gaggtcgatc
     2941 gcattcgcga cgacctgcgc aagctcacgg gcaaagagaa cgtgcagctg aacgtgaacg
     3001 agatcaagca gccggagctg gtggccaagc tggtcgcaca gggcatcggc gaacagctca
     3061 cctcgcgcgt gtcgttccgt cgcgccatgc gtcgcggcgt gcagtcgacg atgcgcgccg
     3121 gcgcgaaggg tattcgcgtg caggtcggcg gccgtctggg cggcgccgag atgagccgca
     3181 gcgagtggta tcgcgaaggc cgtgttccgc tgcacacgat tcgcgccgac gtggactacg
     3241 gcctctacga ggcgcacacc accttcggcc gcatcggcgt gaaggtttgg atctatcgcg
     3301 gcgacatcac cgcgcagcaa caggagaagg aagcgcagat cgctcgcgaa cgcgctcgcg
     3361 cggccgcggc cggcgagatc gtcgcgccgg cgcgtcgcaa ggcgggtggc cgttcggctc
     3421 cgcgcggcgt cgcgcaggac ctgacggcgc agcgttcggc caagccgaag gacgctccga
     3481 aggacgcgcc cgccggcgaa ggcaatccca aggccgagga caaacccaag gccgggaaca
     3541 agccgaaggc cgaggcgcag cccgcggagg cccaggccga ggacaagccg aaggccgagg
     3601 acaagccgaa ggccgaggcg cagcccgcgg aggccaaggc cgagaccgcg cctgccgccc
     3661 aggagtcgaa gtcggaggcc gcaccggccg tcgagacctc agcgcccgat ccggcgcccg
     3721 gcgacgcggc cgcaccgaac acgtccgcga ccgaagggac ggaggcgtaa gtcatgctcg
     3781 ccccgaaaag agtcaagcac cgcaagcagc accgcggccg catgcgcggt caggcgaagg
     3841 gcgccaccac ggtgcacttc ggcgacttcg gcctgcaggc gctcgagccg gcgtggatca
     3901 ccaatcgcca gatcgaggcg gcgcgtgtcg ccatgacgcg aagcatcaag cgaggcggca
     3961 aggtctgggt caacatcttc ccggacaagc ccttcacgaa gaagccggcc gagacgcgca
     4021 tgggctcggg caaaggcaac ccggagtact gggtcgccgt ggtcaagccc ggccggatca
     4081 tgttcgagct ggccggcgtc gacgagtcgg cggcccgtga ggcccttcgc ctcgccgcac
     4141 acaagctccc gatcaagacg cgcttcgtcg cgcggcaaga gggttagatc atgaaggcgt
     4201 ccgaactcaa ggacatggcc gacgacgagc tcgccaccaa gctcggcgag gccaaggaag
     4261 agctgttcaa cctccggttc cagctggtga ccggacagct cgacaacccg atgcggctgg
     4321 gtcaggtgcg caaggacatc gcgcgcatca tgaccaccat ccgcgagcgg gagatcgccg
     4381 cggccgaagc cgccggcgtc ggtgccgaag caggggaggc tcgatgaacg agcgagggag
     4441 ccgcaagacc cgcgtcggcg tggtcgtcag cgacaagatg gacaagaccg tggtggtcgc
     4501 cgtccggtcc accattcgtc acccgttgta cggcaagatc atccgccgcg tgtcgaaggt
     4561 gcaggcgcac gatcaggaca actcgtgcgg cgtcggtgac cgcgtgttgc ttgccgagac
     4621 gcgtccgctt tcgaagacga agcgctggcg cgtcgcagag atcctggaga aggccaagtg
     4681 attcagcaag agtctcgcct caaggtggcc gacaactccg gcgcgaagga gatcctatgc
     4741 attcgcgtcc taggtgggtc ggggcgccgc tatgccggca tcggcgacat catcgtcgcc
     4801 tcggtcaagg acgccatccc cggcgccggc gtgaagaagg gtgaggtcgt gaaggccgtt
     4861 atcgtgcgca ccgccaagga gcgccgccgg ccggacgggt cctcgatccg cttcgacgac
     4921 aacgccgccg tcatcatcaa cgagcagcag aacccgcgcg gaacccgcat cttcggtccc
     4981 gtcgcgcgcg agctgcgcga caagaagttc atgaagatca tctcgctggc tccggaggtg
     5041 ctgtagagat gccgggcctg aaaattcgca aggacgacaa cgtcgaggtc atcaccggca
     5101 aggacaaggg aaagcgcggc cgggtcgtgc gcgtgttccc ggaccgcggc cgcgtgctgg
     5161 tcgagcacgt caacgtcgtg aagcgtcacg agcgcctgcg ggtcggcaag ggccgcggcg
     5221 gcaccgaggg cggcatcatc accaaggaga tgccggtgga catctccaac gtgctggtcg
     5281 tgtgcccggc gtgcgaccgt gggacgcgga ccggatacct gatcgaggac accggtgcga
     5341 agcgccgcgt ctgcaaaaag tgcggaggtg acgtctgatg gcaaccaaga cgatgccccg
     5401 cctcaaggag cggtatcgca gcgagatcgt gccgcatctg atgcaggagc gcgggtacgc
     5461 gaacatcatg atggtgccgc ggctcgagaa gatcgtggtc aacatgggcc tcggcgaggc
     5521 gctcaaggac gccaagatcc tggaaggtgc cgtcgccgac ctgaccgtga tcaccgggca
     5581 gaagccgctg gtgaccaagg cacgcaagtc catcgcgaac ttcaagcttc gcgaaggaca
     5641 ggcgatcggc gcgaaggtca cgctgcgcgg tgaccgcatg tgggagttcg cggaccgtct
     5701 tctgacggtg gcgctgcccc gcattcgcga cttccgcggc ctgcccaaga agctcgaccg
     5761 ccgcggcaac tacaccttcg gcgtgaccga gcagctgatc ttccccgagg tcgactacga
     5821 caagatctac cggacccggg gcatggacat tacgttcgtc accacggcga acgacgacga
     5881 cgaggcccgt gcgctgctga aggcgctcgg cgtccccttc agggagtagc gcatggcgaa
     5941 gaagtgcctt atcgagaagc agcggcgcga gccgaagttc gcctcgcgca agtacagccg
     6001 ttgccagcgt tgcggccggc cgcgggcgta ctaccgcaag ttcggcctgt gccggatctg
     6061 cttgcgccag ctggcgcacg cgggcgaggt tccgggcgtg accaagtcga gttggtgatg
     6121 aagcgatgac tatgaccgat ccgatcgccg acatgctctc gcggattcgc aacgcctcga
     6181 gcgcgcacca cgactcggtc tcgatcccgt cgtccaaaat caaggtcggg atcgccgaca
     6241 tcctcaagcg tgagggctat atcgaggact tccgcatcga gcgcggcgac aaggggccgc
     6301 tgctgatggt ggagctcaag tacggcccga accgtgagcg gacgatcctg ggcatcaagc
     6361 gaatctcgaa gccgggcctg cgtgtgtttg cgcagcgtga cggcatcccg cgtgttctcg
     6421 gcgggctcgg gatcgcgatc atttcgacgt cgaagggtct cctcaccgac aagcaggccg
     6481 ccaaggccgg cgtcggtggc gaggtcgtct gctacgtgtg gtgatggtga gatgagtcga
     6541 atcggacgca agccgatcga gatcccctcg ggggtcgaga tcaagctgaa cggcaggcac
     6601 atcgtggtga agggcccgaa gggcacgctg gagcgtgacc tgccgcacga catcgtcgtg
     6661 aagaaggagg acgcgacgct ggtcgtcgag cgcccgacgg atcagcgcga tcatcgtgcg
     6721 ctgcacggcc tgacgcgctc gctcgtggcg aacatggtta ccggcgtgac cgagggcttc
     6781 aagaaggatc tcgagatcca gggcgtcggg tatcgtgccg cggccaaagg caaggacctg
     6841 gagtttgcgc tcgggttctc gcacccggtg acggtcaccg cgcccgaagg gatcagcttc
     6901 gaagtcccgg ctccgaccaa gatcacggtc tcgggcgccg acaagcaggt cgtcggtcag
     6961 gtcgccgcgg agatccgcgc gttgcggccg ccggacgcct acaaaggcaa gggaatccgg
     7021 tacgccggcg aatacgttcg caagaaggcc ggaaaggcgg cgaagtagac gtggacgcga
     7081 agacgaagca ggttgcgcgg ctgcggcggc accaccgcgt gcgcaagcgc gtgacgggga
     7141 cgcaagagcg cccgcgtctg gcggtgttcc gctcgaaccg gcacatctat gcgcaggtga
     7201 tcgacgac
//
LOCUS       DATLFO010000033         5841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_21141, whole genome shotgun sequence.
ACCESSION   DATLFO010000033 DATLFO010000000
VERSION     DATLFO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21141"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VM841_01120"
     CDS             complement(<1..291)
                     /locus_tag="VM841_01120"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852810.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVL88812.1"
                     /translation="MAEAVIVAAARTPIGRAGKGSLKDARPDDLAAFAVRKAVEKVPQ
                     LNPSEIVDVMVGCGFPEGEQGMNLGRRVALLAGLPDSVPGTTVNRFCASSLQT"
     gene            479..922
                     /locus_tag="VM841_01125"
     CDS             479..922
                     /locus_tag="VM841_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88813.1"
                     /translation="MEPKRIVILAHRTAAGPHLQRVVKSRMSEGRCHFTLIVPAQAPE
                     GFVWTEHQAAAETQVVLDEALEIFRGLGADIEGIVGDRRPLLALDDHLRHDDADEIIV
                     STFSSGASRWLRQDLPHRVARTFDVKMTHVVDERMPADQGRRASA"
     gene            complement(898..1167)
                     /locus_tag="VM841_01130"
     CDS             complement(898..1167)
                     /locus_tag="VM841_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88814.1"
                     /translation="MDDAEKVAALMSANGLAPEETWVGRPEHRYDPDELCTLHTRYGR
                     RNQRWLSLGDEARRSTLAAFRASLDERPELLVWRPEVVYALARRP"
     gene            complement(1167..1559)
                     /locus_tag="VM841_01135"
     CDS             complement(1167..1559)
                     /locus_tag="VM841_01135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88815.1"
                     /translation="MSEVAARYTGIAGTYDAMWSPVIRPMARPAIERLPSGASLVLDV
                     GCGTGALFADLGAAAPRARIVGVDATEAMLRHTRGTVVCGDATRMPLAASSADAAISS
                     STRPARTGTACSMTPARRSRPRLTTGGR"
     gene            complement(1556..2701)
                     /locus_tag="VM841_01140"
     CDS             complement(1556..2701)
                     /locus_tag="VM841_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVL88816.1"
                     /translation="MRGPSDLLAWRSEFPAVERTVFLGSHTLAPASRRTRAAIDRFMD
                     AWESKASAERVWFDDVIPEMRRLESMAASIIGADPPDVALSPSVTTAIASLASALSFD
                     EPKNEIVLSRREFPTDSLAWLAHEGRGAAVVWVDGSEAGDYTAAISDRTAAVTASRVS
                     YLDGAVTDAAAICAAAREAGALSVIDDFHGAGIVPVDVGELGCDALVFGPYKYLFGTS
                     NVGILYVRPDVAKRLRPTITGWFAQRDFFAFDGSRIDWPHSAQRFALGTPAALSIFAS
                     AAGMSIVLDVGVARIRERSLELTGYLIERADECGLAVRTPREPARRGGLVAVEVPDSK
                     RVLDSLLDRGIIVDERHGALRICPHFFNSEDDIDALFDALAALGIGG"
     gene            complement(2698..3702)
                     /locus_tag="VM841_01145"
     CDS             complement(2698..3702)
                     /locus_tag="VM841_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVL88817.1"
                     /translation="MSRVVLLRSVELFDGSRLVPGRRDLLVRNGRIAAVEAAGSVEPP
                     AGARVTEGGAVLPGLVDAHVHVSFSTAEAIVRGGVTGALDLGEPIEAAFAPHPPLRVR
                     AAGPLLTAPGGYPTRTWGANGYGLEVAGEHEAREAVAMLAGRGAAMIKVAITQEPSLD
                     AAAVAVIVEAAAARGLRVAAHALRQADARVALAGGVDVLAHTPVEPLGDDTIAAFASR
                     GTTVISTVLAFGGAEATRVNLMALAAAGCTIAYGTDLGNDGIEPGANAEELSIVAEAC
                     GGAEAALRAATAGAAALAGLAPARVRVGEPANIIVCDGLGFAHLNRPSMIWIDGEEIA
                     "
     gene            3860..4168
                     /gene="groES"
                     /locus_tag="VM841_01150"
     CDS             3860..4168
                     /gene="groES"
                     /locus_tag="VM841_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVL88818.1"
                     /translation="MAFSLEPLEDRVVVRPLEEEVQKTPTGIIIPDTAKEKPQEGEVV
                     AVGPGRFEDGKRVPMDVGVGDRVIYSKYGGTEVKVEGSEYLVLSARDLLAVVKTGKKK
                     "
     gene            4227..>5841
                     /locus_tag="VM841_01155"
     CDS             4227..>5841
                     /locus_tag="VM841_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88819.1"
                     /translation="MNLRRVAPLIALALLVGVAPPAAAQRPITDRLGIDIGLVEISGK
                     PGGQSGDRFKLINKTDTPVEVTAIVEDLGTDARGELVAVPRGSTDLPSAASWVSVAGS
                     DITVPANGTAAVDATIKVPAGAKAGGYYATITFDVDDGGPPESRLAHVILARINGAGF
                     APAGRLVSVEMATPSLSGRVPIEVTVENSGNLHAVGGGFVDIADTFGSQIARIPIPTV
                     VVLPQRQRVVKVVYDSPPVAGRLKATATISFRSGESSASDAGYVLIWWLAVMLAAVLA
                     LFAVSLIRIAGLAGPKAKARRAARAPVPAPAPVPVPAGKADKKRLGRKKREETASAAA
                     APAAAAPEAAATAASPPLAAPPPPDTAPPVSSPGEMPKPPSVPQRDESFWGPGDDDMP
                     APRVRDEPDVEPVEDQEPRPSPFASWALEDDEDSDADEDEDGGEDEEQYFGDEDEDEG
                     PAAGDEDGLAAGDEDAAGGDLDEEDDLGEEDDEDDEDDEDDEREDDAVAQDEAVAAHE
                     AWWDEEEDAEGDRVPAAPAPIAADDDDPAA"
BASE COUNT          907 a         2071 c         2078 g          785 t
ORIGIN      
        1 ggtctgcagg ctcgacgcgc agaagcggtt gaccgtcgtg ccgggaacgc tgtcgggcag
       61 gcccgcgagc aacgccacgc ggcggccgag gttcatgccc tgctcgccct cggggaagcc
      121 gcagccgacc atcacgtcta cgatttcgct cggattgagc tgggggacct tctcgacggc
      181 cttgcggacg gcgaacgccg cgagatcgtc cggccgtgcg tccttgagcg atcccttgcc
      241 ggcgcgcccg atcggcgtgc gcgccgcggc gacgatgact gcctctgcca tgacggtcct
      301 ccctgaagat gtcgaggaca tcattcaatg acatgcgggc ggtccaagca acgtgaggct
      361 tgggtcactc gttgtccggt atgggacccg cctgaagtaa gtttttccca gggagggctt
      421 cccaagggtg ttcggccgtc gtataccgga tatgagggag aagaaaagga ggctcgatat
      481 ggagcccaag cgaatcgtga ttctggctca ccgcacggcc gcgggcccgc atctgcagcg
      541 cgtcgtcaag tcgcggatgt cagagggtcg ctgccacttc acgctcatcg tcccggccca
      601 agcaccggaa ggcttcgtgt ggaccgagca tcaggccgcc gccgagacgc aggtcgtgct
      661 cgacgaggcg ctcgagatct tccgaggcct gggggccgac atcgagggca tcgtgggaga
      721 ccggcggccg ctgctcgcac tcgacgacca tctgcgccac gacgatgccg acgagatcat
      781 cgtctcgacg ttctcgtcgg gcgcgtctcg gtggctgcgg caagacctcc cgcatcgcgt
      841 ggcgcgcacc ttcgacgtga agatgacgca cgttgtggac gagcgaatgc cggccgatca
      901 gggacgccgc gcgagcgcgt agacgacctc gggtcgccac acgagcagct cgggccgctc
      961 gtcgagcgag gcgcggaacg cggcgagagt gctgcggcgc gcctcgtccc ccaaggacag
     1021 ccaccgctga ttgcggcggc cgtagcgcgt gtgaagcgtg cacagctcat ccgggtcgta
     1081 gcgatgctcg ggccggccca cccaggtttc ttcgggcgcg agaccgttcg cgctcatcag
     1141 cgccgcgacc ttctcggcgt cgtccatcag cgcccgccag tcgtcagcct cggccggctg
     1201 cgtcgcgccg gcgtcatcga gcatgcggtc ccagtgcgag ccggcctcgt gctcgacgag
     1261 atggccgcat ccgcggacga ggccgccagc ggcattcgcg tggcatcgcc gcagaccacg
     1321 gttccgcgcg tgtgccgaag catcgcctcg gtcgcatcga cgcccacgat gcgcgcacgc
     1381 ggagcagcag cgccgaggtc ggcgaagagc gcgccggtcc cgcatcccac gtcgagcacg
     1441 agcgaagcgc ccgaggggag ccgctcgatc gccggccggg ccatcggccg gatgaccggc
     1501 gaccacatcg catcgtaggt gccggcgatc ccggtgtagc gcgccgcgac ctcgctcatc
     1561 cgccgattcc cagcgccgcg agcgcatcga agagcgcgtc gatgtcgtct tcgctgttga
     1621 agaagtgcgg gcagatgcgg agcgcgccgt ggcgctcgtc cacgatgatg ccgcgatcca
     1681 gcagcgaatc gagcacgcgc ttcgagtccg gcacctccac cgcgacgagc ccgccgcgtc
     1741 gcgcgggctc gcgaggcgtg cggacggcca gcccgcactc gtcggcgcgc tcgatcaggt
     1801 agccggtgag ctccagcgag cgttctcgaa tgcgcgcgac gccgacgtcg agcacgatcg
     1861 acatgccggc ggccgaggcg aagatcgaca gcgccgcggg cgtgcccagc gcgaagcgct
     1921 gcgcgctgtg gggccagtcg atccgcgagc cgtcgaaggc gaagaagtcg cgttgcgcga
     1981 accagccggt gatcgtcggg cgcagccgct tcgcgacgtc gggccggacg tagaggatgc
     2041 cgacgttgct ggtcccgaac agatacttgt acgggccgaa caccagcgcg tcgcagccga
     2101 gctcgcccac gtccacgggc acgatgccgg cgccgtggaa gtcgtcgatg accgacagcg
     2161 cacccgcctc gcgagccgcc gcgcagatcg cggcggcgtc ggtgacggcg ccgtccaggt
     2221 acgacacgcg gctcgcggtc acggccgcgg tgcggtccga gatcgcggcc gtatagtcgc
     2281 ccgcctcgga gccgtccacc cacacgaccg ccgcgccgcg gccctcgtgc gcgagccatg
     2341 cgagcgagtc ggtcgggaac tcgcgccggg agagcacgat ctcgttcttc ggctcgtcga
     2401 aggaaagcgc ggaggcgagc gaggcgatcg cggtggtcac cgacggcgag agcgcgacgt
     2461 cgggggggtc ggcgccgatg atcgaggcgg ccatcgactc gagccggcgc atctccggga
     2521 tgacgtcgtc gaaccagact cgctcggcgc tcgccttgga ctcccacgca tccatgaagc
     2581 ggtcgatcgc cgcgcgcgtc cgtcgcgacg cgggtgcgag cgtgtgcgag cccaggaaga
     2641 ccgtgcgctc gaccgcggga aactcgctgc gccacgccag cagatcggac ggacccctca
     2701 tgcgatctcc tccccgtcga tccagatcat cgacggccgg ttcaggtgtg cgaagccgag
     2761 gccgtcgcac acaatgatgt tggcaggctc accaacgcgg acgcgagccg gcgcgaggcc
     2821 ggcgagcgcc gcggcgccgg cggttgccgc gcgaagcgcc gcctcggcgc ctccgcacgc
     2881 ttcggccacg atcgaaagct cctcggcgtt cgcgcccggc tcgatgccgt cgttaccgag
     2941 gtcggtgccg taggcgatcg tgcatcccgc ggccgcgagc gccatcaggt tcacccgcgt
     3001 cgcctcggcc ccgccgaagg cgagcaccgt cgagatcacg gtcgtgccgc gcgaggcgaa
     3061 ggccgcgatc gtgtcgtcgc cgagcggctc gaccggcgtg tgtgccagga cgtcgacgcc
     3121 gccggcgagc gcgacgcgcg catcggcctg gcggagcgcg tgggccgcga cccgcagccc
     3181 gcgcgccgcg gcggcctcga cgatcaccgc cacggcggcg gcgtcgagcg acggctcctg
     3241 cgtgatggcg accttgatca tggcggcgcc gcggccggcg agcatcgcga ccgcctcgcg
     3301 cgcttcgtgc tcgccggcga cctcgaggcc gtagccgttc gcgccccagg tgcgggtcgg
     3361 atagccgccc ggcgcggtca gcagcggccc ggccgcgcga acccgcagcg gcgggtgggg
     3421 cgcgaacgcg gcctcgatcg gctctccgag atccagcgcc cccgtgaccc cgccgcgaac
     3481 gatcgcctcc gcggtcgaaa acgacacgtg cacgtgcgca tccaccagcc ccggaaggac
     3541 cgctccgccc tcggtcaccc tggcccccgc cggtggctcg accgatccgg cggcctccac
     3601 ggctgcgatc cgcccgttgc ggaccagcag gtcgcggcgg cccggcacca ggcgagagcc
     3661 gtcgaagagc tcgacggagc gaagcaggac aacccggctc acggacgaag cgtaccgccg
     3721 gcggcggagc ccctccggcc gcgaggccct cgattgactc cctccggagc aagtcgctac
     3781 tatgccgctt agcactcgcc acccctgagt gctaaccacg aggacttgca ggatcattcg
     3841 gtttctaggg agggtgtata tggcgtttag tctcgagccg ctcgaggatc gggtcgttgt
     3901 ccgaccgctc gaggaagagg ttcagaagac cccgaccggc atcatcattc ccgacacggc
     3961 gaaggagaag ccgcaagagg gagaggtcgt ggccgtcggc ccggggcgtt tcgaggacgg
     4021 caagcgcgtc ccgatggacg tcggcgtggg cgaccgcgtg atctactcga agtacggcgg
     4081 caccgaggtc aaggtcgagg gatccgagta cctggtcctg tcggcccgcg acctgctcgc
     4141 ggtcgtcaag accggcaaga agaagtagcc ggcattccgc acaccgacgg gcggcttcgt
     4201 gccgcccgtc gcgtattctt tcccgcatga atctccgacg cgtcgcgccc ttgatcgcgc
     4261 tcgctctgct cgtcggcgtg gctccgcccg cggccgcgca gcggccgatc accgaccgtc
     4321 tcgggatcga catcggtctg gtcgagatct ccggaaagcc gggcggccag tccggtgacc
     4381 gcttcaagct gatcaacaag accgacacgc cggtcgaggt caccgcgatc gtcgaggacc
     4441 tcggcaccga cgccagaggc gagctcgtcg ccgttccgcg cggcagcacg gatcttccgt
     4501 ccgccgcatc gtgggtgagc gtagccggct ccgacatcac cgtacccgcc aatgggaccg
     4561 cggccgtcga cgccacgatc aaggttccgg ccggcgcgaa ggcgggcggc tactacgcga
     4621 ccatcacctt cgacgtcgac gacggcggcc cgccagagtc gcgtctggcg cacgtgatcc
     4681 tggcgcggat caacggggcc gggttcgcgc ccgcgggacg cctggtctcg gtcgagatgg
     4741 cgaccccgtc gctgtcgggc cgcgtcccga tcgaggttac ggtcgaaaac agcggcaacc
     4801 tgcacgcggt cggcggtggt ttcgtggaca tcgccgacac cttcggctcg cagatcgcgc
     4861 gcatcccgat cccgaccgtg gtcgtgctgc cgcagcgcca gcgcgtcgtg aaggtcgtct
     4921 acgactcgcc gccggtcgcg ggacggctca aggcaactgc gaccatctcg ttccgaagcg
     4981 gcgagagctc cgcgagcgat gcgggctacg tcctgatctg gtggctcgcc gtgatgctcg
     5041 cggcggtgct ggcgctgttc gccgtgtcgc tcatccgtat cgcggggctt gcgggcccga
     5101 aggccaaggc ccgccgcgcc gcccgcgcgc ccgttccggc tccggccccc gttcccgtgc
     5161 cggccggcaa ggccgacaag aaacggctcg gccgcaagaa gcgtgaggaa accgcgtccg
     5221 cggccgccgc ccccgcggcc gccgcccccg aggccgccgc cacggcggcc tcgcctccgc
     5281 tcgccgctcc gccgccgccg gacacggctc caccggtctc gtcgccgggc gagatgccga
     5341 agccgccgtc cgtgccgcaa cgtgacgagt ccttctgggg acccggcgac gatgacatgc
     5401 cggccccgcg ggtccgcgac gagcccgacg tcgagccggt cgaggaccag gagcctcgtc
     5461 cgtcgccgtt cgcgtcatgg gcgttggagg acgatgagga ttcggacgcg gacgaagacg
     5521 aggacggcgg agaggacgaa gagcagtact tcggggacga ggacgaagac gaaggcccgg
     5581 ctgccggcga cgaggacggt ctcgctgcgg gcgatgagga cgccgccggc ggcgatctcg
     5641 acgaagagga cgatctcggc gaagaggacg acgaggacga cgaggacgac gaggacgacg
     5701 agcgcgaaga cgacgctgtt gcgcaggacg aggccgttgc ggcccacgaa gcctggtggg
     5761 acgaagagga ggacgccgaa ggcgaccgcg tccccgcagc cccggcaccg atcgcagccg
     5821 acgacgacga tcccgcggcc g
//
LOCUS       DATLFO010000034         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_119644, whole genome shotgun sequence.
ACCESSION   DATLFO010000034 DATLFO010000000
VERSION     DATLFO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119644"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VM841_01160"
     CDS             <1..201
                     /locus_tag="VM841_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVL88820.1"
                     /translation="FDTLANVLFMLGVRTDQAAIAVTGSGLYPILPAVMGIVRHGERL
                     APNQYAGIVVVVAGLVALGLRA"
     gene            complement(198..1658)
                     /locus_tag="VM841_01165"
     CDS             complement(198..1658)
                     /locus_tag="VM841_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVL88821.1"
                     /translation="MGRVRILSIALIAVVLAACNVVPPDVAKQIIPERTEYKTLEAAY
                     HVLLERHVDRPSPEQLLNSALDGAESYLKRKPAEATPAPSVQPNLMTAQPSGTPAPLP
                     PQARGAACVDLKIDRPKFTGSTESDLVKASEALDKAAASCKTADRTHLERAAIDGMAK
                     GMNECHTYYLDPDRAKSFNQPPQPYSGIGATILAPSQSGGMSEISSVFPESPAERAGV
                     RPGDRIKSVNGEDVTGFAPEEIAAKIRGPEGTEVRLVLVRGTQDVTFTIRRATLTPPR
                     VVQREFESGKILMLSVSQLNGDVARQTKDRVERGVAAGAKGYILDLRNNPGGDLSAAV
                     DIASIFGRSITLVDQVGRDGRKEQVRTNDRMFVDTKGAPVVVMVNERSASGSEIIAAG
                     LRANGIATVIGVRSAGCVGIAQPRQMPDDGLLLVTLARMTDVKTGEELNGNGRGVRPD
                     VEAKDVADTAEREDETVALNHLRDKIAAAPGGVGRP"
     gene            complement(1713..2312)
                     /locus_tag="VM841_01170"
     CDS             complement(1713..2312)
                     /locus_tag="VM841_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL88822.1"
                     /translation="MAVQVAQLNAAVIPTTDGASAWGPVPDVIERARAGDRAAFAELY
                     DQYVDSVYRYLLYRLREPSDAEDLTSEVFTRAFANIHRYRWQGKSFLAWLYTIARNAV
                     TDRRRRERPTVDLDTAYGVAEEGPTAHDRAVLGEQVGALRGAVQHLTAEQQQVLSLRF
                     EQNLSSRDVARMLGKNEGAIRALQFRALGRLRKILRDQV"
BASE COUNT          299 a          846 c          887 g          402 t
ORIGIN      
        1 ttcgacacgc tcgcgaacgt cctcttcatg ctcggcgtcc gcaccgacca ggcggcgatc
       61 gcggtcaccg gcagcggcct ctacccgatc ctgcccgcgg tgatggggat cgtgcgccac
      121 ggcgagcggc tcgcgccgaa ccagtacgcc ggcatcgtcg tcgtggtcgc gggcctcgtc
      181 gcgctcggac tgcgcgccta gggccttccg acgcctccgg gggcggccgc gatcttgtcc
      241 cgcaggtggt tgagcgcgac cgtctcgtcc tcgcgctcgg ccgtgtccgc cacgtccttc
      301 gcctcgacgt ccggacgcac gccgcggccg ttcccgttca gctcctcgcc cgtcttcacg
      361 tcggtcatgc gcgcgagcgt gacgaggagg aggccgtcgt cgggcatctg ccgcggctgc
      421 gcgatgccca cgcagcccgc gctgcgcacg ccgatgacgg tcgcgatgcc gttcgcgcgc
      481 aggcccgcgg cgatgatctc tgacccggag gcgctgcgct cgttcaccat caccacgacc
      541 ggcgcgccct tggtgtcgac gaacatgcgg tcgttcgtgc ggacctgctc cttgcggccg
      601 tcgcggccga cctggtccac gagcgtgatg ctccgcccga agatgctcgc gatgtccacg
      661 gcggccgaca ggtcgccgcc ggggttgttg cgcagatcga ggatgtagcc cttcgcgccc
      721 gcggcgacgc cgcgctccac gcggtccttc gtctgccgcg cgacgtcgcc gttcagctgc
      781 gagacggaga gcatcaggat cttgccgctc tcgaactcgc gctggaccac ccggggcggc
      841 gtgagcgtgg cgcggcggat cgtgaaggtc acgtcctgcg tcccgcgcac gagcacgagc
      901 cgcacttcgg tcccctcggg gccgcggatc ttcgcggcga tctcctccgg cgcgaacccg
      961 gtgacgtcct cgccgttcac gctcttgatg cggtcgcccg ggcgcacacc cgccctctcc
     1021 gcggggctct ccgggaagac gctcgagatc tccgacatgc caccggactg gctcggcgcc
     1081 aggatcgtgg cgccgatgcc cgagtacggc tgcggcggct ggttgaagga cttggcgcgg
     1141 tcggggtcga ggtaataggt gtggcactcg ttcatgccct tcgccatgcc gtcgatcgcc
     1201 gcgcgctcga ggtgcgtacg gtccgccgtc ttgcacgagg ccgcggcctt gtcgagcgcc
     1261 tcggacgcct tcacgaggtc ggactcggtg ctgccggtga acttcgggcg gtcgatcttg
     1321 aggtcgacgc aggccgcccc gcgcgcttgc ggcgggagcg gtgccggcgt tcccgacggc
     1381 tgcgccgtca tgaggttcgg ttgcaccgac ggcgcgggcg tggcctcggc gggcttgcgc
     1441 ttgaggtacg actccgcgcc gtcgagcgcg ctgttcagga gctgctccgg ggatggccgg
     1501 tcgacgtgtc gctccaggag gacgtggtac gcggcctcga gcgtcttgta ctcggtgcgc
     1561 tcggggatga tctgcttcgc gacgtccggc ggaacgacgt tgcacgcggc gaggacgacg
     1621 gcgatgagcg cgatggagag gatgcgcacc cgacccacgt gaaaagtgta ggtgtctgcg
     1681 tcggggcgat ctaagagttc gtgaagagcg ctttagacct ggtcgcggag gatcttgcgc
     1741 aggcggccga gcgctcggaa ctgcagcgcg cggatggcgc cctcgttctt cccgagcatg
     1801 cgcgcgacgt cgcggctcga gaggttctgc tcgaagcgca gcgagaggac ctgctgctgc
     1861 tcggcggtga ggtgctgcac cgcgccgcgc agcgcgccga cctgctcgcc cagcactgcg
     1921 cggtcgtgcg cggtggggcc ctcctcggcg acgccgtacg cggtgtcgag gtccacggtc
     1981 gggcgctcgc ggcgacggcg gtcggtgacg gcgttgcgcg cgatcgtgta cagccaggcg
     2041 aggaatgact tgccctgcca gcggtagcgg tggatgttcg cgaacgcgcg ggtgaagacc
     2101 tcgctcgtca ggtcctcggc gtcgctcggc tcgcggagcc ggtagaggag gtaccggtag
     2161 acgctgtcga cgtactggtc gtacagctcg gcgaacgcgg cgcggtcacc ggcgcgggcg
     2221 cgctcgatca cgtcggggac cggcccccag gcgctggcgc catcggtcgt cgggatcaca
     2281 gcggcgttca gctgcgcgac ctgcactgcc acccttccag ttccctccac aagcccgttg
     2341 gtccgcgtgg aagatcatgg cgaccggacg tggccgcgcc aagccgtccg ccgtaccaaa
     2401 cgcgtggcag gggcgtttcc aggcgggcgc ggcc
//
LOCUS       DATLFO010000035         7109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_90551, whole genome shotgun sequence.
ACCESSION   DATLFO010000035 DATLFO010000000
VERSION     DATLFO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90551"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..2551
                     /locus_tag="VM841_01175"
     CDS             212..2551
                     /locus_tag="VM841_01175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HVL88823.1"
                     /translation="MISRRGKLASVLVVALLSGPFAPPGQGAAATEPASLVDPFIGTL
                     GAGFVFPGPAAPYGMVQLSPDTEGYFAYTGYQWFDTHIRGFSHVRVQSMGVPEGGEVP
                     FMPTTGAVVTDVNSYQSPFSHATESAEPGYYQVTLPREGIRAELTAGTRVGMHRYTFP
                     AVEQANVLLDIGRQIPGGDLNQRLQREAGTNRASVRIVDDRTVVGTTNVDRASAFGYR
                     VHFAAVFDRPFTSSGVWASRGAPLQPGVTQITGQGAGAAVTFDARGDQDVIVKVGISF
                     VSEANAMANLAAEAPGFDFDGLREATFDAWNDALSVIDVTGGTPAEQISFSTALYHAQ
                     HHPNVFNDVNGDYIGHDDQVHRIGDAGDPMPAGSTYYANFSMWDTYRAEMPLLAWIVP
                     DRYRDMMRSLAAIVRWGGQMPRWAMMNRNPNFMNGEPALPVIADGFCRGLVPDDALAT
                     LYGEARRVALDARRNPEFYSRGYTRDASNTLEYALADFALALMADRLGHTADRDRLLA
                     QSGSWRNVFDPETRFVRPRNDNGSWYGDPYRPEMPDHFTEGTGWQYTWLVPQDVAGLS
                     DAIGGDEEVRRRLDVFFSTALNATVPVAGPEAQQKATLYGIAYYGNQYAPSNEHDLQA
                     PWLYNWTSEPWKTQAIQRAYQGLYRATPDGLPGNDDLGTMSAWFIWSALGLYPVTPGS
                     PTYTIGSPLFTRAVVHPPGSPPFTIDALGASAADKYVQSAALDGAPLHRTWITHDALT
                     GGATLHLPMGPVPSSWGSGISARPPSSSTTQLADFACQP"
     gene            complement(2542..3075)
                     /locus_tag="VM841_01180"
     CDS             complement(2542..3075)
                     /locus_tag="VM841_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88824.1"
                     /translation="MTADPSTGLPVLVVAYCPGERVVGVTLREFDADSYAPGRPMWQV
                     WAIGDGRQLPRIAVGETPAGFRETVALDLPSPVELLELTATFDDDSEDGVVFTIAELK
                     PGILLRYGEPTDERELHKDADAACSLNPLTGFGLPGWLFVVFAAGSIASLVVIVHDAV
                     RRRRRLSNMNLSAGGYG"
     gene            complement(3304..3377)
                     /locus_tag="VM841_01185"
     tRNA            complement(3304..3377)
                     /locus_tag="VM841_01185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3341..3343),aa:Met,seq:cat)
     gene            complement(3468..6317)
                     /locus_tag="VM841_01190"
     CDS             complement(3468..6317)
                     /locus_tag="VM841_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HVL88825.1"
                     /translation="MRVSTGTTAPRISRRLLAFIAAGIVVALIVLGSAATVYTDLLWF
                     TEAGFNEVFWTQIWTKVGLAAVFGVVFAAVLLANLWVAQKITKPSHVFSMRDQVIERY
                     RLMLRPYVRRLVIGGSILFGLFAGSSASGQWRNWLLFSNAESFGQTDPVFGKDLGFYV
                     FKLPFHQFLFTWGFSTLLLITMIVAGTHYLLGGIRTEGAPPRVAPHVKAHLSVLLGLL
                     VASKAWAYRLDQFNLLYSPRGGQVTGASYTDVNAQLPALRLLVVIAVVCAVLFLVNIR
                     FRGWVLPAAGLGILLITSIIAGGIYPATVQRLRVNPQERDREAPYIERNIAATREAFG
                     LGDEDVTVKSLPAGAQLTQAMLRENQPTVENLRLWDPQVLQRAYTQLQRITNYYEFLD
                     IDVDRYTIDGRLRQVMLSAREMAPSGLPSGARTWVNNHLVYTHGYGIVASRVDRATAQ
                     GQPSFIVSDIPPQGGAKAIPGAPAITEPRIYYGESEEQAFVVVRSANEELDYPVGQTS
                     FKSSNYEGTGGIELSGILRRAAFAWRFRDPNLLISGAIRADSRIMFNRQISQRVARVA
                     PFVQLDHDPYLAIVDGRFVWIQDAYTTTANYPYSQRVNLAELTGGHLGGGANYIRNSI
                     KATVDALNGTVTLYMWDPDDPIIRTWSKVFPGLIKPVSEMPEDVRTHVRYPEDLFRVQ
                     ADRYATYHITDPIDFYSKNDVWRIPQDPTAADGSLMPPYYVLMKLPGEEEAEFVLMLP
                     FAPFSQTGSTATRRQNMTSWLAARMDGDKYGKLESFQYTKETLVFGPEQVATLINNDQ
                     GVRNVISALDPREQRIRWGNLLVVPIGEAVLYVRPLFLESSESALPEFKRVVVVAGER
                     IRISETIEGALAALFGEEPEGEPEGPAGKDVAALIAEAAEHFRLADEALRAGDFARFG
                     REQKAGREALNEAARLSGATPSPSPSPSPSPSPSP"
     gene            complement(6385..>7109)
                     /locus_tag="VM841_01195"
     CDS             complement(6385..>7109)
                     /locus_tag="VM841_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88826.1"
                     /translation="AGTIDLPALREYTIGLTVPDVRLVVVDEGDRWDRRYLADAIQID
                     QALGPYERSFDVNAGVKRPTLEFIGTFVDPSAIGFLGPLLDTRLARTGPFDALVSLLV
                     AIEHANVVNTALPAAFGDVLDLRDQGASFGIVAQALSVEPGPLLRRLLLALNPAEPPQ
                     KITPIEGTPTPRPGASGGSDPTPSPSPSGPSRPTSSPRPTSSPSPSPSPSGSPSPSPS
                     PSPTCEIVDQLMGGCTPPPIIP"
BASE COUNT         1203 a         2435 c         2396 g         1075 t
ORIGIN      
        1 gcgcgaccgg cggcgagccg aaacgcgacg gcggtccgtc cgggtacgtc tcattgtttt
       61 ccggaaggct catcgttcgg gatctcgcat ctggccgggc cggccgtggg cggcctggcg
      121 atcgcgggaa tcggcctgac cgggacctat atctggatga gaacgcgcct ccgcgaacag
      181 gaatgagcga ttcggccgcg aattggagac catgatctcg cgtcgcggaa aactcgcctc
      241 tgtcctcgtc gtcgcgctcc tgtcaggacc gttcgcgccg ccagggcaag gggccgcggc
      301 gaccgagcct gcatccttgg tcgacccgtt catcgggacg ctcggcgccg gcttcgtctt
      361 tcccggtccg gcggccccct acgggatggt ccagctctcg cccgacaccg aggggtactt
      421 cgcgtacacg ggctatcaat ggttcgacac gcacatccgc ggcttcagcc acgtgcgggt
      481 gcagtccatg ggcgtgccgg aaggcggaga ggttccattc atgccgacga ccggtgcggt
      541 cgtgaccgac gtgaatagct atcagtcgcc gttttcgcat gcgaccgagt ccgccgagcc
      601 gggctactac caggtgacgc tgccccgcga aggcatccgc gccgagctga ccgcgggcac
      661 gcgggtaggg atgcaccgct acacgttccc ggccgtggag caggcgaacg tgctgctcga
      721 catcggccgg cagattcccg gcggcgacct gaaccagcgg ctgcaacgcg aggcaggcac
      781 gaatcgagcg tccgtgcgca tcgtggacga ccgcacggtc gtgggaacga ccaacgtcga
      841 tcgcgcgtcc gcgttcggct accgcgtgca tttcgcggcg gtgttcgacc ggccgttcac
      901 gtcctcgggc gtgtgggcct ctcgcggcgc gccgctccag cccggggtga cgcagatcac
      961 gggccaagga gcaggcgctg cggtgacgtt cgacgcgcgc ggggatcagg acgtgatcgt
     1021 caaggtcggg atctcgttcg tctccgaggc gaacgcgatg gccaaccttg ccgccgaggc
     1081 gcccggcttc gacttcgacg ggctgcgcga ggcgacgttc gatgcgtgga acgacgctct
     1141 gtcggtgatc gacgtcaccg gcggcacccc ggccgaacag atctcgttct ctaccgcgct
     1201 ctaccacgcg cagcatcatc cgaacgtctt caacgacgtc aacggcgact acataggcca
     1261 cgacgatcag gtgcatcgca tcggcgacgc cggcgatccg atgcccgcgg gctcgaccta
     1321 ctacgccaac ttctcgatgt gggacaccta ccgtgccgag atgccgctgc tcgcatggat
     1381 cgtgcccgac cggtatcgcg acatgatgcg ctcgctcgcg gccatcgtcc gctggggtgg
     1441 tcagatgccg cgctgggcga tgatgaaccg caacccgaac ttcatgaacg gcgagccggc
     1501 gctgccggtg atcgcggacg gcttctgccg cgggctcgtt cccgatgacg cgctggcgac
     1561 actgtacgga gaagcgcgcc gcgtcgcgct ggacgcgcgc cgaaaccccg agttctactc
     1621 ccgcggctac acccgcgatg cgtcgaacac gctcgaatac gcgcttgcgg acttcgcgct
     1681 ggcgttgatg gccgatcgcc tcggccatac cgcagaccgc gaccggctgc tcgcgcagtc
     1741 cggatcctgg cgcaacgtgt tcgatcccga aacccggttc gtgcggccac gcaacgacaa
     1801 cggttcttgg tacggcgatc cctaccgtcc cgagatgccg gatcatttca ccgaagggac
     1861 gggctggcag tacacctggc tggtgccgca ggacgtcgcg gggctctccg acgcgatcgg
     1921 cggcgacgag gaggtgcggc gccgtctcga cgtgttcttc tcgaccgcgc tcaacgcgac
     1981 cgtgccggtg gcggggccag aagcgcagca gaaggcgacg ctgtacggca tcgcctacta
     2041 cgggaatcag tacgcgccgt cgaacgagca cgacctgcag gcgccgtggc tctacaactg
     2101 gacgagcgag ccgtggaaga cgcaagcgat ccagcgcgcg tatcaggggc tctaccgcgc
     2161 gacgcccgac ggcctgcccg gcaacgacga tctcggcacg atgagcgcgt ggttcatctg
     2221 gagcgcgctg ggtctctatc cggtcacgcc cggctccccc acctacacca tcggcagtcc
     2281 gctcttcacc cgcgccgtcg tgcatccgcc gggctcgccg ccgttcacga tcgacgcgct
     2341 gggcgcatcg gcggcggaca aatacgtgca gtcggcggcg ctggacggcg cgccgctgca
     2401 ccggacgtgg atcacacacg acgcgttgac cggcggcgcg acgctgcatc ttccgatggg
     2461 acccgtccca tcatcgtggg ggtcgggcat atcggcccgg ccgccatcat cctcaacgac
     2521 acaactcgcc gacttcgcct gtcagccgta gccgcccgcc gaaagattca tgttggaaag
     2581 gcgtctgcgg cgccggaccg catcatgaac gatcaccacg agcgacgcga tcgagcccgc
     2641 agcgaacacc acgaaaagcc atccgggcag gccgaagccc gtcaacgggt tgagcgaaca
     2701 cgccgcatcc gcatccttgt gtagttcgcg ctcgtcggtc ggctcgccgt atcgcagcag
     2761 gattcccggc ttcagctcgg cgatcgtaaa caccacgccg tcctcggagt cgtcgtcgaa
     2821 cgtcgcggtc agctcgagca gctcaacggg tgagggaagg tccagagcga cggtctcgcg
     2881 aaagcccgcc ggcgtttccc cgacggcaat ccgaggcaac tgccgcccgt cgccgatcgc
     2941 ccagacctgc cacatgggcc ggccgggagc gtaggagtcc gcatcgaact cgcggagcgt
     3001 tacgcccacg acgcgctcgc cgggacaata ggcgacgacg agcacgggca accccgtcga
     3061 aggatcggcc gtcaccgcag ccagcgtgat cggcgagcac gcccccaagc ctcccgccaa
     3121 tagcacgagt gcggggacga tgcgaattcg gcctgcatgc caacgcaagg gagccatcac
     3181 ccaacgatac gaccttagag aaccatccga cgcagagcga cgccccggct tcgtgcccgg
     3241 ccgcatcggc gaaaagaaag aggccctcac tgacggaggg cctctgacct gcggttctct
     3301 ttgtagcgcc ggcaggattt gaacctgcga cctctgggtt atgagcccag cgagctaccg
     3361 gactgctcca cggcgcgtcg taggacccat cttaccggac ccgggggccg aggcaaccgg
     3421 aaagcatccc ggcctgctat ccgcctgccg gccgccgggc cctggcgtca gggtgatggg
     3481 gacggcgacg gggaaggaga cggggatggc gacggcgtgg caccggacag gcgcgcggcc
     3541 tcgttgagtg cctcacgccc ggccttctgc tcgcggccga accgggcgaa gtcgcccgcg
     3601 cgcaacgcct cgtcggccag acggaaatgc tcggccgcct cggcgatcag cgcggccaca
     3661 tccttcccgg cgggcccttc cggctcgcct tcgggctctt ccccgaacag cgccgccagc
     3721 gccccctcga tcgtctcgct gatccggatc cgctcgcccg ccacgaccac gacgcgcttg
     3781 aactcgggca gcgccgactc ggacgactcc aggaacagcg gccgcacgta cagcaccgcc
     3841 tcgccgatcg gcacgaccag caagttcccc cagcggatgc gctgctcgcg cggatcgagc
     3901 gccgagatca cgtttcgcac cccctgatcg ttgttgatca gcgtcgcgac ctgctcgggc
     3961 ccgaacacca gcgtctcttt cgtgtactgg aacgactcga gcttgccgta cttgtccccg
     4021 tccatccgcg ccgcgagcca cgaggtcatg ttttgacgcc tcgtcgccgt cgaacccgtc
     4081 tgcgagaacg gcgcgaacgg cagcatcaaa acgaactcgg cttcctcttc ccccggcagc
     4141 ttcatcagca cgtagtaggg cggcatcaag ctgccgtcgg cggcggtcgg atcctgcggg
     4201 atgcgccaca cgtcgttctt cgaatagaag tcgatcggat cggtgatgtg ataagtggcg
     4261 tagcggtcgg cctgcacccg gaacagatcc tcgggatagc gcacgtgcgt gcgcacgtcc
     4321 tcgggcatct ccgagaccgg cttgatcaag cccgggaaca ccttgctcca cgtgcgaatg
     4381 atcggatcgt cgggatccca catgtacagc gtcacggtcc cgtttaacgc gtcgacggtc
     4441 gccttgatgg agttgcggat gtagttggcg ccgccgccca gatggccgcc ggtcagctcg
     4501 gccaggttca cacgctgcga gtagggatag ttcgcggtgg tggtgtaggc atcctggatc
     4561 cacacgaacc gcccgtcgac gatcgcgaga tacggatcgt ggtccagctg cacgaacggc
     4621 gcgaccctgg ccacgcgctg cgagatctgc cggttgaaca tgatccgcga gtcggccctg
     4681 atcgctcccg agatcaacag gttcgggtcc cggaaccgcc acgcgaacgc ggcacgccgg
     4741 aggatgcccg acagctcgat accgccggtc ccttcgtagt tcgacgactt gaagctggtc
     4801 tggccgaccg gatagtccag ctcttcgttg gccgagcgca ccacgacgaa cgcctgctct
     4861 tcactttcgc cgtagtagat gcgcggctcg gtgatcgccg gcgcgccggg aatcgccttc
     4921 gccccgccct gcggcggaat gtcgctgacg atgaacgacg gctggccctg ggcggtcgcg
     4981 cggtccacgc gcgaggcgac gatgccgtag ccgtgcgtgt agaccagatg gttgttgacc
     5041 caggtgcgcg cacccgacgg cagcccggac ggcgccatct cgcgcgcgga cagcatgacc
     5101 tggcgcagcc gcccgtcgat cgtgtagcga tcgacgtcga tgtcgaggaa ctcgtagtag
     5161 ttggtgatgc gctgcagctg cgtgtaggcg cgctgcagaa cctgcgggtc ccacaggcga
     5221 aggttttcga cggtcggctg gttctcgcgc agcatcgcct gcgtcagctg cgcgccggcg
     5281 ggcagcgact tcaccgtgac atcttcgtca ccgagcccga acgcctcgcg ggtcgccgcg
     5341 atatttcgct cgatgtaggg cgcctcgcgg tcgcgctcct gagggttgac ccgcagccgc
     5401 tgcacggtcg cgggatagat gccgccggcg atgatcgagg tgatcaaaag gatgccgagc
     5461 cccgcggcag gcagcaccca gccgcggaag cggatgttga ccaggaacag gaccgcgcac
     5521 acgaccgcga tcaccaccag cagccgcagc gccggcagct gcgcgttgac gtcggtatag
     5581 gacgcgccgg tgacctgacc gccgcgcggc gagtagagca ggttgaactg gtccaggcgg
     5641 tacgcccacg ccttcgacgc gaccagcagg cctagcagga ccgacagatg cgccttgacg
     5701 tgcggggcga cccgcggcgg cgcgccttcg gtccggatgc cgccgagcag gtagtgcgtt
     5761 cccgccacga tcatcgtgat cagcagcagc gtcgagaagc cccaggtgaa caggaactgg
     5821 tggaacggca gcttgaacac gtagaagccg aggtccttgc cgaacaccgg atcggtctgg
     5881 ccgaacgact cggcgttgga gaacagcagc cagttgcgcc actgcccgga ggcgctcgac
     5941 ccggcgaaga gcccgaacag gatcgagcca ccgatcacga gccggcgcac gtagggccgc
     6001 agcatcagcc ggtagcgctc gatcacctga tcccgcatcg agaacacgtg cgagggcttg
     6061 gtgatcttct gggcgaccca gaggttggcc agcagcacgg cggcgaatac gacgccgaat
     6121 acggccgcga gcccgacctt ggtccagatc tgggtccaga acacctcgtt gaagccggcc
     6181 tcggtgaacc aaagcaagtc cgtatagacg gtcgcggccg agccgagcac gatcagcgcg
     6241 accacgatgc cggcggcgat gaaggcgagc aggcgccggc tgatgcgcgg cgccgtcgtt
     6301 ccggtggaga ccctcacgga cgcgaggcta acagaccggc gggcccggtg ccgggcgctc
     6361 ggttccctac gggcgctcgg ttccctacgg gatgatcggc ggcggcgtgc agccgcccat
     6421 gagctgatcg acgatctcgc acgtgggcga cggagacggt gacggcgagg gcgagcccga
     6481 cggtgacggg ctgggcgacg gcgaggacgt cggacgcggc gatgaggtcg gccggctcgg
     6541 gccgctcggc gacggcgacg gggtcggatc cgagccgccg gaggcgcccg gacgcggcgt
     6601 gggcgtgcct tcgatcggcg tgatcttctg cggcggctcg gcgggattga gcgcgagcag
     6661 caggcgccgc agcagcggcc ccggctcgac cgacagcgcc tgggcgacga tgccgaagct
     6721 cgccccttgg tcgcgaaggt cgagcacgtc cccgaacgcc gcgggaagcg ctgtgttgac
     6781 gacgttggcg tgctcgatcg cgaccagcag cgatacgagc gcgtcgaacg gcccggtgcg
     6841 cgcgagccgc gtgtccagca gcggcccgag gaagccgatc gcactcggat cgacgaacgt
     6901 gccgatgaac tcgagcgtgg gccgcttcac gcccgcgttg acgtcgaagc tgcgctcgta
     6961 gggaccgagc gcctggtcga tctggatcgc gtcggccagg taccggcgat cccaacggtc
     7021 cccttcgtcg accacgacca gccgcacgtc cggcacggtc agcccgatcg tgtactcgcg
     7081 cagcgcgggc aagtcgatcg tgcccgcgg
//
LOCUS       DATLFO010000036         5072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_30869, whole genome shotgun sequence.
ACCESSION   DATLFO010000036 DATLFO010000000
VERSION     DATLFO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30869"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1704)
                     /locus_tag="VM841_01200"
     CDS             complement(190..1704)
                     /locus_tag="VM841_01200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVL88827.1"
                     /translation="MATPTIQTKTTRAMRWPLVLLVAFAALVPRVANPSEAITIDETL
                     WIDRSANFIEAVFDGRLDDAWASGHPGVTTMWIGGLAQKTLPENAPLRDRYARARIAM
                     AVADVGLLVLVWWLAASLFGLFAGSAGAMLLAFDPFLIANQRVLHLDGLLTVAMAASL
                     LALMRALRLNDRGWLRWSGVLAGVAFLTKQPSAFLVPATLILLWRDGSGIKQRFLHWL
                     WPAVAVFVVAWPVIWVWPWKPLSLLISSTGKGATDAGDAFFLGRHVRDPGPLFYPVAF
                     IWRATIVTLPAVIAATVWAARRRATEPAARDALRLLALGLGFVVMMTVGAKKGDRYGL
                     PGYGLAYLAAGIFIAHQLRGRSRAVIAAAAIALAALHAAPGLALHPYEQESYNWAVGG
                     PATAKHVLTVGWGDGLDEAARDLHRMPELSGATVATTRMHPFVDFYEGDVIRIEESAL
                     MTPDGPQPDLVLFYLSSLQTGRYDTIWARYRDAEPLHEIRVAGLPLARVYRAAS"
     gene            1738..3936
                     /gene="pcrA"
                     /locus_tag="VM841_01205"
     CDS             1738..3936
                     /gene="pcrA"
                     /locus_tag="VM841_01205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HVL88828.1"
                     /translation="MDSASTAAGPLDLLADLNPPQRDAVAHGDGPLLVVAGAGSGKTK
                     VITNRIAYLIRERHESPFSILAITFTNKAAAEMKERVGRLVGDRLGNAMWVMTFHSAC
                     ARLLRREAARLGYTSSFTIYDASDSERLVRFCMDDLEIDTRRFTPRQIHNAIGKLKDD
                     LVDEDTFAERASSYVERAISDVYRAYQARLRKANAMDFDDLIFNTVHLFRLFPDVLTG
                     YQDRFKHILVDEFQDTNPAQFRLVAMLAGRDRNVCAVGDLDQSIYKFRGADFRNVMKF
                     EEEFPDTRVITLEQNYRSTQTILAAANAVIENNRMRKPKNLWTDSGGGDKIVRYLGDD
                     ERDESIWITREIQRLHDDEGYAFRDIAIFYRTNAQSRVLEEIFMRYGMPYKVIGSLKF
                     YDRKEIKDAIAYLRVLINPADEVSLKRIINVPKRGIGEQTVAALERFAGAQEIPLGVA
                     IDRVDEIEDLQNRARGGVAEVAGLLTLLRERVERNDGVAEILEATLERSGYVAELESE
                     RSIEALGRVENLKELIGVAREFDEANAELGLREFLEQISLVSDTDELEDESGAVTMMT
                     VHIAKGLEFPAVFIVGLEDGVFPHARSMADPDELEEERRLIYVGITRAKKRLYLTHAF
                     SRSLWGGSNYNPPSRFLHEIPTELIEPAGRDAGGGSSGGGSRIAARPTPEPKGGTLNA
                     VAVGDEVYHDVFGKGIVSAVSGMGGNAEATIEFAGGPKRLLLAYAPLQKT"
     gene            4035..4544
                     /locus_tag="VM841_01210"
     CDS             4035..4544
                     /locus_tag="VM841_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL88829.1"
                     /translation="MAESSLETGESLRRLERLYVRHAGPAIRLAYLLCGDRHLAEDLV
                     QDAFVRLAGRFADLRDADAFQAYLRRTVINQAKMHGRRRNLERRRLEELSTRPAPHAT
                     DTDSVADRATLRAALLALPYRQRAAIVLRYYADLSEAQAAEALGCGPAALRSLVARGM
                     QTLREKVSR"
     gene            4550..>5072
                     /locus_tag="VM841_01215"
     CDS             4550..>5072
                     /locus_tag="VM841_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88830.1"
                     /translation="MESEIRELLRSMGTEVPEPAAPPPGLRSRARRRRARNALAAGLA
                     SAVLVYGAAALASGVFDRRDVAVPAACDQDWTSETIAVNATPLVAVEVIASDDVWIAG
                     QRPGGGNGSLGSAGLVMRHWDGTSWETASVSIDGASDFSVFSISAAASDDVWAAGSIT
                     HGESYESRRVEGLL"
BASE COUNT          881 a         1688 c         1763 g          740 t
ORIGIN      
        1 atctgggccc ggggccggtg tcaggccggt gtcaggccgg tgtcaggccg gggcccggct
       61 cgcgggagca aggaccctgg atcccgagag ggggtcccca taccctgtgc aaccaagttg
      121 cagagggtgt ggttgcaact tggttgcaca gggtccggga ggggtcaccc ggtggcgggc
      181 ggaggccggc tatgaggcgg cgcgatagac gcgggcgagg gggaggccgg cgacgcggat
      241 ctcgtggagc ggctccgcgt cccggtagcg ggcccagatc gtgtcgtagc ggccggtctg
      301 caggctcgag aggtagaaca gcaccagatc gggctgcggg ccgtctggcg tcatcagagc
      361 gctttcctcg atccggatga cgtcgccctc gtaaaagtcc acgaacgggt gcatgcgggt
      421 ggtggcgacg gtggcgccgg agagctcggg catgcgatgc aggtcgcggg cggcctcgtc
      481 caggccgtcg ccccagccga cggtcagcac atgcttggcg gtggcgggac cgccgacggc
      541 ccagttgtag gactcctgct cgtagggatg cagggccagg ccgggcgcag cgtgcagcgc
      601 ggccagggcg atcgcggcgg cggcgatgac ggcgcgggag cggccgcgca gctgatgggc
      661 gatgaagatg ccggcggcca gatacgccaa gccgtagccg ggcaggccgt agcgatcgcc
      721 cttcttggct ccgacggtca tcatcacgac gaagcccagg ccgagcgcca gcaggcgcag
      781 cgcatcgcgg gcggcgggct cggtggcgcg gcggcgggcc gcccagaccg tggcggcgat
      841 cacggccggc agcgtcacga tggtggcgcg ccagatgaag gcgaccggat agaacagcgg
      901 gcccggatca cggacgtgac ggcccaggaa gaacgcgtca cccgcgtcgg tcgccccttt
      961 tccggtgctg gagatcagca gcgaaagcgg cttccacggc cacacccaga tcaccggcca
     1021 cgcgaccacg aagactgcga cggcgggcca cagccaatgc aggaagcgct gcttgatgcc
     1081 gctgccgtcg cgccacagca ggatcagcgt cgccggaacc aggaacgcgc tcggctgctt
     1141 ggtgaggaac gcgacccccg caagcacacc gctccaccgc agccagcctc gatcgttcag
     1201 ccgaagcgcg cgcatcagcg cgagcagcga tgccgccatc gcgaccgtca gcaggccgtc
     1261 cagatgcagc acgcgctggt tcgcgatcag aaacggatcg aatgccagca acatcgcgcc
     1321 ggccgaaccc gcgaacaacc cgaacagcga ggccgcgagc caccagacca acaccagcaa
     1381 gcccacgtcc gccaccgcca tcgcgatgcg ggcgcgcgcg tagcggtcgc gcaagggtgc
     1441 gttctcgggc agcgtcttct gcgccaggcc gccgatccac atcgtggtca cgcccggatg
     1501 gccgctcgcc cacgcatcgt ccaagcgtcc gtcgaaaacc gcctcgatga agttcgcgct
     1561 gcgatcgatc cacagcgtct cgtcgatcgt aatcgcctcg gatgggttcg ccacccgcgg
     1621 aacgagcgcc gcaaacgcga ccagcagaac caacggccag cgcatcgcgc gggtcgtctt
     1681 ggtctgaatc gtgggtgtcg ccatcgccgg tcggccggat catagataat gccggtcatg
     1741 gactccgcat ccacggccgc cgggccgctc gatctgcttg ccgacctgaa tccgccgcaa
     1801 cgggacgcgg tcgcgcacgg cgacgggccg ctgctggtcg tggccggcgc gggaagcggc
     1861 aagaccaagg tcatcaccaa ccggatcgcg tacctgatcc gcgaacggca cgaatcgccg
     1921 ttctcgatcc tggcgatcac cttcaccaac aaggccgcgg ccgagatgaa agagcgcgtc
     1981 ggccggctgg tcggggaccg gctgggaaac gcgatgtggg tgatgacgtt ccactcggcg
     2041 tgtgcgcggc tgctgcgccg cgaagccgca cggctgggct acacgtccag cttcacgatc
     2101 tacgacgcct ccgacagcga gcggctggtc cgcttctgca tggacgacct cgagatcgac
     2161 acgcgccggt tcacgccccg tcagatccac aacgcgatcg gcaagctgaa agacgatctg
     2221 gtggacgaag acaccttcgc cgagcgcgcc tcgtcgtacg tcgagcgcgc gatcagcgac
     2281 gtctatcgcg cgtaccaagc ccggctgcgc aaggcgaacg cgatggactt cgacgacctg
     2341 atcttcaaca ccgtgcatct gttccggctg ttcccggacg tgctgaccgg ctatcaggac
     2401 cgcttcaaac acatcctggt ggatgagttc caggacacca atccggcgca gttccgactg
     2461 gtggcgatgc tggccggccg cgaccgcaat gtctgcgcgg tcggcgacct ggaccagtcg
     2521 atctacaagt tccgcggtgc cgactttcgc aacgtcatga agttcgaaga agagtttccg
     2581 gacacgcgcg tgatcacgct cgagcagaac tatcgctcga cgcagacgat cctggccgcc
     2641 gcgaacgcgg tcatcgaaaa caaccggatg cgcaagccga agaacctgtg gaccgactcg
     2701 ggcggcggcg acaagatcgt ccggtatctg ggcgacgacg agcgcgacga gtcgatctgg
     2761 atcacccgcg agatccagcg cctgcacgac gacgaaggct acgccttccg ggacatcgcg
     2821 atcttctacc ggaccaacgc gcagtcgcgc gtcctggaag agatctttat gcgctacggc
     2881 atgccctaca aggtcatcgg ctcgctcaag ttctacgacc gcaaagagat caaggacgcg
     2941 atcgcgtatc tgcgcgtgct gatcaacccg gccgacgagg tctcgctcaa gcgcatcatc
     3001 aacgtgccca agcgcggcat cggcgagcag accgtcgccg ccctggaacg attcgcgggc
     3061 gcgcaagaga ttccgctggg cgtcgcgatc gaccgcgtgg acgagatcga agacttgcag
     3121 aaccgtgcgc gcggcggcgt cgccgaggtc gccgggctgc tgacgctgct gcgcgaacgg
     3181 gtcgaacgga acgacggcgt cgccgagatt ctggaggcga cgctggagcg cagcggctac
     3241 gtcgccgagc tggagtccga gcgctcgatc gaagcgctcg gccgcgtcga gaacctcaaa
     3301 gagctgatcg gcgtcgcccg cgaattcgac gaagcaaacg ccgagctggg cctgcgcgag
     3361 ttcctcgagc agatctcgct ggtatccgac accgacgagc tggaagacga gtccggcgcc
     3421 gtcacgatga tgaccgtgca catcgccaaa ggccttgagt ttccggccgt gttcatcgtc
     3481 ggcctggaag acggcgtgtt tccgcacgcc cgctcgatgg ccgacccgga cgagctggaa
     3541 gaggaacgcc gcctgatcta cgtcggcatc actcgcgcga agaagcgcct gtatctgacg
     3601 cacgcgttca gccgcagcct gtggggcggc agcaactaca acccgccgtc acgctttctg
     3661 catgagatcc cgaccgagct gatcgagccc gcgggccgtg acgcgggcgg cggttcgtcg
     3721 ggcggtggct cgcggatcgc cgcgcggccc acccccgaac caaagggcgg aacgctaaac
     3781 gcagtcgccg tgggcgacga ggtgtaccac gacgtgttcg gcaagggaat cgtcagcgcg
     3841 gtcagcggca tgggcggcaa cgccgaggcc accatcgaat tcgccggcgg ccccaagcgc
     3901 ctcctgctcg cgtacgcccc gctgcagaag acatagcagc agcgagcccc gtggcgacgc
     3961 ggggcggcct tcgaagtggc tccgcaggcg accgacaccg aacggcctcg ccgtgcatct
     4021 aggatgcgat ggaggtggcg gagagctctt tggagaccgg cgagtcgctg cggcggctcg
     4081 agcggctcta cgtgcggcac gccggtcccg cgatcaggct ggcgtacttg ctttgcggtg
     4141 accggcatct ggccgaagat ctggtgcagg acgcgttcgt gcggctggcg ggccgcttcg
     4201 cggacctgcg tgacgccgat gcattccaag cgtacctgcg gcgcaccgtc atcaaccaag
     4261 cgaagatgca cggccggcgc cgaaacctgg agcgccggcg tctcgaagag ctctcgaccc
     4321 gtccggcgcc gcatgccacc gacaccgact cggtggccga ccgcgccacg ctgcgcgcgg
     4381 cgttgctggc gctgccctat cggcagcgcg ccgcgatcgt ccttcgctat tacgccgacc
     4441 tgtccgaggc gcaggccgcc gaggcgctcg gctgcgggcc ggccgcgctg cgatcgctcg
     4501 tcgcgcgcgg catgcaaacg ttgcgtgaga aagtctcgag gtgattgtcg tggaatccga
     4561 aatccgagag ctgcttcggt cgatgggcac cgaggtgccc gagccggcgg cgccgccacc
     4621 cggcttgcgg tcgcgcgccc ggaggcgtcg cgcccgcaac gcgcttgccg ccggtctggc
     4681 atcggcggtg ctcgtctatg gcgcggccgc gcttgcctcg ggcgtattcg atcgtcgcga
     4741 cgtggctgtg ccggccgcgt gcgaccagga ttggacgtcg gagacgattg cggtgaacgc
     4801 gactccgctc gtcgctgtcg aggtgatcgc ctccgacgat gtgtggatcg cgggtcaacg
     4861 gccgggcggt ggcaacggct ctctgggatc ggccggactg gtcatgaggc attgggatgg
     4921 aacctcgtgg gaaaccgcct ccgtgtcgat cgacggagca tcggacttct cggtgttttc
     4981 gatcagcgcc gccgcctcgg acgacgtgtg ggcagccggt tcgatcaccc acggcgagtc
     5041 ctacgagtcc cggcgggtcg aagggttgct gc
//
LOCUS       DATLFO010000037        22017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_100206, whole genome shotgun sequence.
ACCESSION   DATLFO010000037 DATLFO010000000
VERSION     DATLFO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100206"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VM841_01220"
     CDS             complement(<1..646)
                     /locus_tag="VM841_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL88831.1"
                     /translation="MKIAAVVLNFRRPGDTIACVESIHRDAGDVAVTVVDNASGDGSA
                     EHIAGALPGIHLIASEANRGYAAGNNLGIRRTLAGDAGAVLILNNDLTLEPGCVDALR
                     GALTANPSWGVAAPLSLLSGPGDVVDFFTGEVLLDHAALRAPGRDEPRGERFREPAQT
                     GYVTGSAMLVRREVLERAGLLDERYFLVWEDVEYSLRVRKAGYALGVTPAAVVRH"
     gene            complement(643..1311)
                     /locus_tag="VM841_01225"
     CDS             complement(643..1311)
                     /locus_tag="VM841_01225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVL88832.1"
                     /translation="MKRSFARALSRLHRKLHAFEEREDAPAFGAKGPGSVVKMPSMLL
                     NPERLHLGRDSYIHPYCRIEIITANPHLDGPPLPPVDARIVIGDRVVINSFTHFGAMQ
                     RITLGDDVGIASGVCIEDHHYLFEQASTERPLKKQPFRVAPVTLEQGCMIGEHAVILP
                     GVTVGANSWVGANAVVTEDVPAYSIVAGVPAKVIRRMDPASGEWERVRAAVRKPAAPR
                     KKRS"
     gene            complement(1319..2191)
                     /locus_tag="VM841_01230"
     CDS             complement(1319..2191)
                     /locus_tag="VM841_01230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88833.1"
                     /translation="MKTDEHTHTDTPVCPCGSTSYKTVLTADRYCVYGWNVQPMDYEL
                     WKCTRCRLVRTWPEPPHEEHHIFRDDSFLDSYLQNPELYEHYLRVTVGEIAKVKPAPG
                     RFLDVGANIGTQLRIAGELGYDAVGVELNTAACEYAQGQGLNVINAACEDAGLADESF
                     DVISLSATAEHIPDFGEELGLYRRLLKPGGLLHLSNSPNYRSFGAWYEKDLWYGIQPT
                     GHVWQFTPKTLKDVVRRCGLKPVYVRTYNLHRDFGRGKKERLRKKAFAVAQTLGLGDA
                     LSVGAVKPGRTSGL"
     gene            complement(2188..2976)
                     /locus_tag="VM841_01235"
     CDS             complement(2188..2976)
                     /locus_tag="VM841_01235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL88834.1"
                     /translation="MTVSAVVIAMNERDMIAAAIASCRFADEILVVDGGSIDGTQDIA
                     RTAGARVVERPFDDFARQRSFALSEAAGEWVLFVDADERVTPALAREILAAVAGSSHA
                     GYKLPRRNMVLGRWLDWHFGGEDAPVRLVRRAVARLQPQGVHEVIEVDGSVGALTNKL
                     VHLTHRSVTDLVDKINRYSSIEAAELAAKGARAPTRRAIAASFPKAFVRYWRSGLGKE
                     DTVGAIEAALLAFNRTLVLAKLWEQTRAEPMEQTYAKAEAEATR"
     gene            complement(2973..3842)
                     /locus_tag="VM841_01240"
     CDS             complement(2973..3842)
                     /locus_tag="VM841_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88835.1"
                     /translation="MSVTLRLACPACGRDDWKPDLAAGAHALVRCRCGLRALDRAPDA
                     GAALHDNENMYGAEEYNAWYRTMRDVLTARYRGDLDEIERLHPSRGAILDVGCSYGWF
                     LQVAAERGWTTAGIEATDATAREARQAGLDVRTGTIEAAGYDEASFDVVCLWDVLEHV
                     PQIDTFLAEIRRVLRPGGLLAINSPNIASVMARQAGADWSWLLLPHHVWQFTPAAMRA
                     TVQTRGFAVERSYTWEPPEAFVQDLKLYKRRPRLGAKAVRRVTDKLLVPAEKAWCRLG
                     YGGLIRLYARRAP"
     gene            complement(3839..4723)
                     /locus_tag="VM841_01245"
     CDS             complement(3839..4723)
                     /locus_tag="VM841_01245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88836.1"
                     /translation="MRNTKEMPPVAGLIRPCIVCASTERSVELRLGDYEIHRCPCGLR
                     TLSPEPAEETLVEVFDDGTIYSEAESLRVHVLEQNHRSLYDVEKFVGPGRLLDVGCGL
                     GYLLESARARGWEAVGVDPSPFSVRRAREKGFEAHRGLLHELGLPAASFDAISLLQVV
                     EHLIDARALLAECRRLLKPGGALLVETPNPASLLAAVKREGFNYWIPPVHCVWYTPDA
                     LGRVLAAAGYTPVKVSTWSARTPVLHDGIDVVASTKLGRRLPGRMRRATGSAVGALAD
                     AFGRGSIVEAVALRWEDA"
     gene            complement(4716..6341)
                     /locus_tag="VM841_01250"
     CDS             complement(4716..6341)
                     /locus_tag="VM841_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HVL88837.1"
                     /translation="MCGWLGRPDPIDAASHGDEAPGIVGPLGLTPDAEAGLTAAGAAA
                     LEQAADEAGITRTLVEEDTMSAGEVRRLAVRGIGAVLVRTFSLRAMTFAGNIILARIL
                     APETFGLFAIVNFVVLISAFLADMGIGAALVQRKERLTEEDLRTAFTLGFIVDAAITA
                     LLMLLAPVLVAAYNLEPQYANAVRVMALTIMVSTFAIVPAIQMERNLRFKEMANADLV
                     SQIVYVFTAVGLAASGLHVSAFVIASLASRTLHTVIVNIISPWKPVLGVVRARLRAML
                     AFGVPYQLNGLVLQLKDNFVPTFIAIVSGAQAVGYINWAVGLATNALFLLTIVSRVTF
                     PAYARLQHDLPALKDSIEKSIKWISATVFPAVFMLAALAHQIVRFLYGPKWAPGLISF
                     YLLCIPMIASSYSTVLVSALHGVGRAKTVLKLSTIWAVAGWAISVPLTLWIGYNGFAL
                     GMAFVSLLSVLSVIEMNKIVKVTFVRDQLKIALIAGLPAFAVWVMSYQVVHGIPSLLG
                     LGAFGGLSYIGLMALTGEIREARRLVKGSLQRA"
     gene            complement(6322..7479)
                     /locus_tag="VM841_01255"
     CDS             complement(6322..7479)
                     /locus_tag="VM841_01255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL88838.1"
                     /translation="MKILVVSAGIVSDRRPHGEALIAFNILSRLARRGHEVTAYVTEY
                     DDEIPGVTFKTISAHAPGAALSRLAFARRAARAARSEQADVHHLLLPLTTDEGYTTAS
                     GAPLVVGPLMLPWPAGDGGAKPRNRLISGVARLTLPRYEQHLHARTMRRASSLMVTCR
                     PALAAIDHDLRGRCVEVPYGVDTTRFTPSPLPGDPVVLFYSVLLPRKGYRTLLRAFPM
                     VRERIPRARLIMAGDDPRGLAGQILEEAERLGIADAVTLRGPVKPADAPMLFREARVF
                     CQPSFGEPFGMTILEAMASGRPVVATAAGGVPGFVRDGEHGRLVQPGDERLLADALTS
                     TLVRREAERIGAHNREVAVARYDWERVVDRIEAAYAAAAAGKESPRVRMAG"
     gene            complement(7476..8408)
                     /locus_tag="VM841_01260"
     CDS             complement(7476..8408)
                     /locus_tag="VM841_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL88839.1"
                     /translation="MNVLVTGGAGFIGSHLVEALVARGDTVTVLDDLRGGTIANLDAV
                     AGDVHFEEGDLLGADLPALLAGGGFDTVFHLAGTAYVPPSVEDPVYDFRLVAEGTLKV
                     CDALRRHSPSSTLVYTSSAAVYGDPESLPITEDHRVAPVSPYGLHKYTAEQSVRLHAE
                     LYGLRTASARLFSVYGPRQRKQVIFDFARKIAASPSRIEAFGDGTQVRDFVYVGDVVR
                     ALLRIASGGPLRGEVYNVATGRGISTGELLAMVAATMNAEPEVVWSGSVRPGDPDKWL
                     ASIERLRSIGYEPATSLEDGIAATVAWARELIQA"
     gene            complement(8405..9139)
                     /locus_tag="VM841_01265"
     CDS             complement(8405..9139)
                     /locus_tag="VM841_01265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88840.1"
                     /translation="MSKLDNMRAFWDTKAQKNPLWYVTTILDYAKPDEEQFWQTGEDE
                     VARVLADAKCDGGEAAAEIGCGVGRLTRPLAKRFCHVHAFDVSPEMVAHAKRNLMSYP
                     GVAASATSGDGTLPLPDASIDFVLSMQVFHHIPDRTITLRYIREAGRVLRPGGAIGFQ
                     LRTCRLSAGPLARLERIARNTVEAARRRRRPPPADLDSAAWHGSRVLIGEIRRAARAS
                     GMQVESVRWLVKDGANIQVICRKRSA"
     gene            complement(9197..10417)
                     /locus_tag="VM841_01270"
     CDS             complement(9197..10417)
                     /locus_tag="VM841_01270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL88841.1"
                     /translation="MPKSPETPHPPPLNLAYVSRSAPPRSGGIEAHMRRVATGLAAGG
                     HRLRIFAARIDDEPFTRLNTTLATQRFEPFLDGEVEIWPVPAGRRARATMLPSAVSKL
                     PGADRIGYDRIRRATLPLVVTALAPGFKRAFFAARLVHCWGGEPLMHAAMRAARQRLL
                     PLVVTPFAHPGHWGDDELNAGLYRNADAVVALLPGEADVYVTLGVAPERVHVIPVAAP
                     PAPESSFDPRAVHGIDTPIVLCLGVKRPYKYRALLHALPHIATRDVRVVFVGPETPES
                     EADFAAATDPRILRRGKVDEAEKWGWLRAANVLCLPSVSEILPVSVLEAWRTGTPVVV
                     SEGRFTRDLVDDGEDGIVCAGEPGPIAQAVETVLKDPAAAERMGRAGAAKVQRHYEPE
                     TVVAQHAGLYASLV"
     gene            10464..11819
                     /locus_tag="VM841_01275"
     CDS             10464..11819
                     /locus_tag="VM841_01275"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HVL88842.1"
                     /translation="MSTNNAWNPSTWRDLPALQQPEWPDAAAVDAVAKELHTWPPLVF
                     AGEARNLQAQLAKVHAGEAFLLQAGDCAESFFDHSADSVRDRLKVLLQMAAVLTYAGG
                     TPIVKVSRMAGQFAKPRSSPMEKIGDAEIPSFRGHIVNSDEPSPGARIPDPRRMLEAY
                     HHAAATLNLLRAFTDGGFADLGKVHQWNNEFVAASPQGRRYEQIATQIDRALAFMRAV
                     GIDTDNPALHQVDVWTSHEALLLEYEEALTRQDSLTGDWYDCSGHFLWIGERTRDPGG
                     AHVTFLAGVGNPIGCKIGPAADPAEVIELCRRLNPDRIPGRLTLITRMGAAKVREALP
                     PILEAVRDAGEPVIWSCDPMHGNTFTSGTGLKTRHLDDIVAEIDGFFAAHRETGTRAG
                     GIHLEITGAAVTECLGGGDNLTEADLDREYTTLCDPRLNAKQSLDLAFHLADLMTQNG
                     A"
     gene            complement(11920..14562)
                     /locus_tag="VM841_01280"
     CDS             complement(11920..14562)
                     /locus_tag="VM841_01280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVL88843.1"
                     /translation="MRARIRPFIVAALLLALSAVGFPAKAASQIRIVRDQFGVPHVYG
                     ATAEDVAFGAGYALAQDRLWQMHIFRLVSKGNLSHLLGSLIVGADKTVRFWTYTASER
                     AARFATYPPDIKRWWLAWVDGINKWIAEAKAEPAAKMPFEFAEFGEPLDEWTIDDSMA
                     MADYLIYTFGSGGGSEVRNLADLQAYIAKFGQAEGRKIWDDLIWINDPDAPLSIPADY
                     DWKSTPSRARPEADLKTLEGDARISLPQAMRVAGPAQGVTPILSQADLLRIPVAGSVL
                     ADVDVLEDGRQTLEKIFFKFGSNAQIVAPHRTTSGNTALQAGPQVGYFLPQALSDFGM
                     HSADGQIDATGMTFAGVGPAVLIGRGTGYNWTTTTGASDITDTFVVQLNPSNAKQYLF
                     DVDGAGPQPAVWENMECRTEVYAQKGVPFDQQEICRTRHGPVLSFDTANNVAYAVRYS
                     WFNREGGTIEGFFRYNQVRSLEDFATYANMLASNHNMFYADDQGNIGYWHPGNFPRRA
                     AGDLRLPFVGTGTQEWQGLLTANEVPHAINLPRGWIANWNNKPSVDWDRENGWGAVHG
                     VQTFLDNLDAAGPARPDPWGGSVNPDRQVDWQDLNANIRYAAFRDFNADYFTQYVPAS
                     SPNAVEQKALEVLRSYNGFIYDANGDKKVDSAGYTILGRFVSDLRAAVFNDDLVGVPN
                     RTSTSTLVHVMNPAAALQPQRNWLGSETRQQVAARALSSAVTALAAQFGNSEPRTWLS
                     DQPEQNYTRLNADLLTSVARDTGRNTVRSNTGVTVGALQPGAVNVPPGNVPNHIRMNR
                     GTYNHLVLYLDPPSGSGILGESRAEHGSVISPGQSGFINLLGQEARHSRDQFDLYVQW
                     RYKPMPMTLAESLALAESDITITR"
     gene            complement(14694..14858)
                     /locus_tag="VM841_01285"
     CDS             complement(14694..14858)
                     /locus_tag="VM841_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88844.1"
                     /translation="MQTPPPVRDRSRRTGPLVMLLAGIAISVVVSLVLRTFVPVFILF
                     LPFVWTGRRR"
     gene            complement(14895..15911)
                     /locus_tag="VM841_01290"
     CDS             complement(14895..15911)
                     /locus_tag="VM841_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HVL88845.1"
                     /translation="MSRSTFTERPLGASSAAPSAPAFSRVAVIVPVYNGAHLIEGCLR
                     ALAAQTLDRREFRIVVVDDGSTDGTQRVVAHLAANLRASVTLIEREHAGPAAARNAGL
                     AATDSTYVAFTDADVEVASDWLERALARLERDAALAGVEGRTLPKGVKGTYTHQMENE
                     RGQLYMTCNMIYRRDAIAGGFDERFRMAFLEDSDVAFGVLERGGRIEFVADVLAHHLV
                     LQEGTVKFWREARKRFYNPLLHKKHPASYRRLLKPVVPAFPAPYVDYVLALAAFVVAL
                     ATGAWWVAVPTGVLAAWTLRRVAHVLRARTPGALIRAAVVPLVQTGWVVAGMIRFRAL
                     DPRI"
     gene            complement(15908..17110)
                     /locus_tag="VM841_01295"
     CDS             complement(15908..17110)
                     /locus_tag="VM841_01295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL88846.1"
                     /translation="MRVLQIGRYGSVKGGAESYVRELCAGLRNAGHEVALAYRFDPDD
                     TRLEVRAGTQIAAITSRNASPAPVEAAEVRRAVEAFAPDVIHVHNAEAAWLAGLCDEI
                     APVLSAVHDHRLDCPTGTRYWAAWNRACDVTPGATCLGYNVAAHCGSLRANATLEPYL
                     RWKRLHAAARRGPRIQVFSGAMRDALGRVGIEAPVEVTPYPATALPAAPPAEPGDARP
                     VVLAHGRATKEKGFDLLLEAMHAVEMPAHLVIAGDGHHLPALRRAAAAVPPRHRVTFL
                     GWTQRDALAGWLRAATVAAVPSAWPEPFGIAGLEAMSVGVPVVASDIGGIREWLDDGA
                     TGLAVPPRDATAFGRAIARLLADATLRERMGAAAKRRIEADFALDAHVARVVEIYERT
                     RRSWKEAA"
     gene            complement(17107..18024)
                     /locus_tag="VM841_01300"
     CDS             complement(17107..18024)
                     /locus_tag="VM841_01300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL88847.1"
                     /translation="MTTKTRGTLPEGLYWAPCPICAKTGGKVVHTEPMGELSSPLTGR
                     FPEDLEMRIVRCKHCGFLYANPRPTQDVLDERYQHMSDIDFNLNGQQARVRMFEHLID
                     RLQAKHPRRGKILDVGCFTGTLLEVAAKRGWEPWGVEINKVASGYAREDLGYSVFTTE
                     LRKAGFEPDFFDAVTMTDVAEHVIDPKETFEEIFRILKPGGMLVMTVPNGSIQIPKEN
                     LKRRLKKGPGVVIGGLGHVNHFSQSSVRGLLRGIGFGKVEVGCMIPERQYTARMDRFK
                     RAYVTVARAVLATTGVHIGHELLVTARKP"
     gene            complement(18021..19163)
                     /locus_tag="VM841_01305"
     CDS             complement(18021..19163)
                     /locus_tag="VM841_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL88848.1"
                     /translation="MSRAGVKQSSVVVVSHACVVDVNQEPFRALAGRGVRVHVIAPKA
                     LRTDIRGTIPFRALDGVDATALPVRIGGFRRGVRQAGVHMILYAGLREAIRGQEPTLI
                     YVEEEPWSFTAMQVDRIARELGVPFAFHQSQNVAKRLPPPFESIRRRVLREAAGATVR
                     LEGAARVLRASGFTGPMLSIPNVIDPSRFDAASPMLPGLPRPVAGFVGRLVEEKGVMV
                     FLRAAAAAKAGCVVVAGDGPLRARAEAEATRLGLDARFLGSVAHDAVAQVFAAVDVLV
                     IPSRTTRGWKEQFGRVVIEANAAGVPVVATECGALGDTVRATGGGIVLAGGDPQGLAA
                     AVAALAHDDQRRRRLGDAGRAAVRARFTPEAVAKDLHEFFARLEDR"
     gene            complement(19160..20335)
                     /locus_tag="VM841_01310"
     CDS             complement(19160..20335)
                     /locus_tag="VM841_01310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL88849.1"
                     /translation="MRILALTPALPWPPHTGGTRRIAAILEGLAASHEISLFALHGEG
                     GSAEATADAPLAVVRTVPYPGLPERGRIRFWTQLLTDPLPKAVRQAFDGDTLAAAAAF
                     AREVRPDAIYAEPIEMEPCLRACAEAVPSARTMMGWIDVVSTNIRRQTERDRGLPHRL
                     HARREFARMVRLERRVAQSVDARTCVSSGDRAALEALTGRPFVLAPNGVDTALFAPRG
                     GAINDRQLLFVGPLSFPPNRDAINWFAHEILPHLGGVTLTVAGEPAGFAAPQGVGLAG
                     RVTDVRHMMARAGAVVVPLRSGSGTRLKILEALAMGKAVISTRIGAEGLEVQHDRDLL
                     LADDPRAFADAVRRVLGDATLRARLGANGRALVERRYRWQDTVRAVEAALAGEGARV"
     gene            complement(20332..21480)
                     /locus_tag="VM841_01315"
     CDS             complement(20332..21480)
                     /locus_tag="VM841_01315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL88850.1"
                     /translation="MKVGICISVYRRTGGLERVAVEWARGLHRRGHDVVVFAQEVIER
                     DPGLTYVPVGGARSPNWRRAATFPRAAAAALRAHPVDVACSFGAAVDAPTVMGTAGPH
                     RAWFEQGRAEAPPWTREGLRRRLNPHHRVVMAVEKRIVGGGRFEMLYATSQRSADDYA
                     RLYGVPDGRVGVLANGVNMDEFRFDPAVRERVRAEWGAGERPVVLTLANEIARKGIDT
                     LIAAFPRVQAAVPGALLVIGGPAGGAEAARLAREAGLSGEDVRTVGRIAEPSEAYAAA
                     DLFAFPTRYDPWGLVAVESLSCGTPVVCGRSAGVAEHVRDGETGILLDAVRDPAALAG
                     AVVRGLALPASRDECRASVAHLSWERILDRVEKILGDVIARREAVGTR"
     gene            complement(21477..>22017)
                     /locus_tag="VM841_01320"
     CDS             complement(21477..>22017)
                     /locus_tag="VM841_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL88851.1"
                     /translation="WKGQHVFLRAAARLTAAHPDARFVVAGDALGGFSAGYPAGLRAL
                     ADELGIAGRVIFTGACDDVPAVLAALDVFVHASVAEPFGIVIVEALAAGVPVVATRGG
                     GVAEIVTDGEDGFLVECGDDEQIAGRVAEILADPALTVRLSNAGRARARAAFTAGRMV
                     EQFTAVLERYAPRRVRERV"
BASE COUNT         3341 a         7616 c         7946 g         3114 t
ORIGIN      
        1 cgtggcgcac gaccgctgcg ggcgtcacac ccagcgcata tccggctttc cgaacccgca
       61 gcgagtactc gacgtcctcc cagaccagga agtagcgctc gtcgagcagc ccggcccgct
      121 cgagcacctc gcgccgcacg agcatcgcgc tgccggtgac gtagccggtc tgggccggct
      181 cgcggaaccg ttcgccgcgc ggctcgtcgc gcccgggagc ccgcagcgcg gcgtggtcga
      241 gcagcacctc gccggtgaag aagtccacga cgtctcccgg accggacagc agcgaaagcg
      301 gcgccgcgac gccccaggac gggttcgcgg tgagcgcgcc gcggagcgcg tccacgcatc
      361 ccggctcgag cgtcaggtcg ttgttgagga tcagcaccgc accggcgtca ccggcgagcg
      421 tgcgacggat gcccaagttg ttgccggcgg cgtacccgcg gttcgcctcg ctcgcaatca
      481 gatgaatccc cggcagcgcg ccggcgatgt gctcggcgga gccgtcgccg gaggcgttat
      541 ccacgacggt caccgcgacg tcgccggcat cgcggtggat cgactcgacg caggcgatcg
      601 tgtcgccggg ccggcgaaag ttcagcacaa cggcggcgat cttcacgaac gcttcttgcg
      661 cggcgcggcc ggcttgcgaa cggcggcgcg cacccgttcc cattcgccgg aggcgggatc
      721 catgcggcgg atcaccttgg ccggcacgcc ggccacgatc gagtacgccg ggacgtcctc
      781 ggtcacgacc gcgttcgcgc cgacccacga gtttgcgccc acggtgacgc cgggcaggat
      841 caccgcgtgc tcgccgatca tgcagccctg ctcgagcgtg accggtgcga cgcgaaacgg
      901 ctgcttcttg agcggccgct cggtgctggc ctgctcgaac aggtagtggt ggtcctcgat
      961 acacacgccg ctcgcgatgc cgacgtcatc gccgagcgtg atgcgctgca tcgcgccgaa
     1021 gtgggtgaac gagttgatga cgacgcggtc gccgatcacg atgcgcgcgt ccaccggcgg
     1081 cagcggcggg ccgtccaggt gcgggttggc cgtgatgatc tcgatgcggc agtacggatg
     1141 gatgtaggag tcgcggccga gatggagccg ctcgggattg agcagcatcg agggcatctt
     1201 caccacgctg ccgggcccct tcgcgccgaa cgcgggggcg tcctcgcgct cttcgaacgc
     1261 gtggagcttg cggtgcaggc gcgacagggc tcgcgcgaag gatcgcttca cggccggatc
     1321 ataaaccgct cgtgcggccg ggcttgaccg cgccgaccga cagcgcgtcg cccaggccga
     1381 gcgtctgggc gacggcgaag gccttcttgc ggagccgctc cttcttgccg cggccgaagt
     1441 cgcggtgcag attgtaggtg cggacgtaga cgggcttgag gccgcagcgg cgcaccacgt
     1501 ccttgagtgt cttgggcgtg aactgccaga cgtgcccggt gggctggatc ccgtaccaca
     1561 ggtccttttc gtaccaggcg ccgaaggagc ggtagttcgg cgagttcgac aggtgcagca
     1621 ggccgcccgg cttgagcagg cggcggtaca gcccgagctc ctcgccgaag tcgggaatgt
     1681 gctcggcggt cgcgctgagg ctgatcacgt cgaaagactc gtcggccagg cccgcgtcct
     1741 cgcaggccgc gttgatcacg ttcaggcctt ggccctgggc gtactcgcac gcggcggtgt
     1801 tcagctcgac gccgacggcg tcgtagccga gctcgccggc gatgcgcagc tgcgtgccga
     1861 tgttggcgcc gacgtcgagg aaacgtcccg gcgcgggctt gaccttggcg atctcgccga
     1921 cggtgacgcg caggtagtgc tcgtagagct cggggttttg caggtacgag tccaggaacg
     1981 agtcgtcacg gaagatgtga tgctcctcgt gcggcggctc gggccaggtg cggaccagcc
     2041 ggcagcgagt gcacttccac agctcgtagt ccatcggctg aacgttccat ccgtagacgc
     2101 agtagcgatc ggccgtcagg accgtcttgt agctcgtcga gccgcacgga cacacgggcg
     2161 tgtccgtatg cgtgtgctcg tccgtcttca tcgagtcgcc tccgcttccg ccttcgcgta
     2221 ggtctgctcc atcggttcgg cgcgggtctg ctcccacagc ttcgccagca ccagcgtccg
     2281 gttgaacgcc agcagcgcgg cttcgatcgc gcccaccgtg tcttccttgc cgagcccgct
     2341 gcgccagtat ctcacgaagg ccttggggaa cgaggcggcg atggcccgcc gggtcggggc
     2401 gcgcgcgccc ttggcggcga gctcggcggc ctcgatgctc gagtagcggt tgatcttgtc
     2461 caccagatcg gtgacggagc ggtgggtcag gtgcacgagc ttgttcgtca aggcaccgac
     2521 gctgccgtcc acctcgatca cctcgtgcac gccctgcggc tgcagccgcg cgaccgcgcg
     2581 gcgcaccagc cgcacgggcg cgtcctcacc gccgaagtgc cagtcgagcc agcggccgag
     2641 caccatgttg cgccgcggca gcttgtagcc cgcgtgcgag gagccggcga ccgccgccag
     2701 gatctcgcgt gcgagcgcgg gcgtgacgcg ctcgtcggcg tcgacgaaca gcacccactc
     2761 ccccgccgcc tcggacaggg cgaagcttcg ctggcgcgcg aaatcgtcga aggggcgctc
     2821 aacgactcgc gcgccagcag ttctggcgat gtcttgggtt ccgtcgatgg atccgccgtc
     2881 cacgaccagg atctcgtcgg cgaagcggca cgaggcgatc gcggcggcga tcatgtcgcg
     2941 ctcgttcatc gcgatcacga cggccgagac ggtcacggcg ccctccgggc gtagaggcgg
     3001 atcagcccgc cgtagccgag acggcaccag gccttctcgg ccgggacgag cagcttgtcg
     3061 gtgacgcggc gcaccgcctt ggcgccgagg cgcggccgcc gcttgtagag cttgaggtcc
     3121 tgcacgaacg cctcgggcgg ctcccaggtg taggagcgct cgacggcgaa gccgcgcgtc
     3181 tgcacggttg cgcgcatcgc ggccggcgtg aactgccaga cgtgatgcgg cagcagcagc
     3241 cacgaccagt ccgcgccggc ctgccgcgcc atgacgctgg cgatgttcgg cgagttgatc
     3301 gcgagcaggc cgccgggcct gagcacgcgg cggatctcgg cgaggaacgt atcgatctgc
     3361 ggcacgtgct cgagcacatc ccacaggcac accacgtcga aggacgcctc gtcgtagccg
     3421 gcggcctcga tcgtgccggt gcgcacgtcc agcccggcct ggcgggcctc gcgtgcggtc
     3481 gcgtcggtcg cttcgatgcc ggccgtggtc cagccgcgtt cggcggcgac ctgcagaaac
     3541 cagccgtagc tgcagccgac gtccaggatc gcgccgcgcg acggatgcag ccgctcgatc
     3601 tcgtcgaggt cgccccggta gcgtgcggtc agcacgtcgc gcatggtgcg gtaccacgcg
     3661 ttgtactcct cggcgccgta catgttctcg ttgtcgtgca gcgccgcgcc ggcgtcgggg
     3721 gcgcggtcga gcgcgcgcag gccgcagcgg caacgcacca gcgcatgcgc gccggcggcc
     3781 aggtccggct tccagtcgtc gcggccgcac gccgggcagg cgagacgcag ggtgacgctc
     3841 acgcgtcctc ccatcgcagt gcgaccgcct cgacgatcga gccgcggccg aacgcgtcgg
     3901 cgagcgcccc gaccgcgctg ccggtggcgc gccgcatacg ccccggcagc ctgcggccga
     3961 gcttcgtcga agccaccacg tcgatcccgt cgtgcagcac cggggtgcgc gcgctccagg
     4021 tcgagacctt gaccggcgtg tagccggcgg cggcgagcac gcggcccagc gcatccggtg
     4081 tgtaccagac gcaatgcacg ggcgggatcc aatagttgaa gccctcgcgc ttgaccgccg
     4141 cgagcagcga ggcggggttg ggtgtttcaa ccagcagcgc gccgccgggc ttgagcaggc
     4201 ggcggcactc ggcgagcagc gcgcgcgcgt cgatcaggtg ctcgacgacc tgcagcagcg
     4261 agatcgcgtc gaaggacgcg gccggaaggc cgagctcgtg cagaaggccg cggtgtgcct
     4321 cgaagccctt ctcgcgtgca cggcgaaccg agaacggcga cggatcgacg ccgaccgctt
     4381 cccatccgcg cgcgcgagcg gactccagca gatagcccag gccgcacccg acgtcgagca
     4441 ggcggcccgg cccaacgaac ttctcgacgt cgtagagcga gcggtggttc tgctcgagca
     4501 cgtggacgcg caacgactcg gcctcggaat aaatggtgcc gtcgtcgaag acctcgacga
     4561 gcgtctcctc ggccggctcg ggcgacagcg tgcgcagccc gcacgggcag cggtgaatct
     4621 cgtagtcgcc cagtcgcagc tcgacggagc gctccgtcga ggcacacacg atgcacggcc
     4681 ggatcaggcc ggcaacggga ggcatctcct tggtgttacg cacgctgcag cgatcctttc
     4741 accaggcgcc gggcctcacg gatctcgccc gtgagcgcca tcaagccgat gtaggaaagg
     4801 ccgccgaacg cgccgagccc gagcagcgac gggatgccgt gcacgacctg atacgacatg
     4861 acccagaccg cgaaggccgg caggcccgcg atcagcgcaa tcttgagctg gtcgcgcacg
     4921 aaggtgacct tgacgatctt gttcatctcg atgaccgaca ggaccgacag cagcgacacg
     4981 aacgccatgc cgagcgcgaa gccgttgtag ccgatccaca gggtgagcgg caccgagatc
     5041 gcccaccccg cgaccgccca gatcgtgctc agcttgagca cggtcttggc ccggccgacg
     5101 ccgtgcagcg cgctgaccag cacggtcgag tacgacgagg cgatcatcgg gatgcacagc
     5161 aggtagaacg agatcaggcc cggcgcccac ttcggcccgt agaggaaccg cacgatctga
     5221 tgcgcgagcg ccgcgagcat gaacaccgcc gggaagaccg tggctgagat ccacttgatc
     5281 gacttctcga tcgagtcctt cagcgccggc aggtcgtgtt ggagccgcgc gtaggcggga
     5341 aaggtcacgc gcgagacgat ggtgagcagg aacagtgcgt tggtcgccag ccccaccgcc
     5401 cagttgatgt agcccaccgc ctgcgcgccg gagacgatcg cgatgaaggt cggcacgaag
     5461 ttgtccttga gctgcaggac gagcccgttg agctggtacg gaaccccgaa ggcgagcatc
     5521 gcgcgcagcc gcgcccgcac gacgcccagc accggcttcc acggcgagat gatattgacg
     5581 atcaccgtgt gaagggtgcg agaggcgagc gacgcgatga cgaaagccga cacgtgcagc
     5641 ccgctcgccg cgagaccgac tgcggtgaac acgtacacga tctgggagac cagatcggcg
     5701 ttggccatct ccttgaagcg caggtttcgc tccatctgga tggccgggac gatggcgaag
     5761 gtcgagacca tgatggtcag cgccatcacc cggaccgcgt tcgcgtactg cggctcgagg
     5821 ttgtaggcgg ccaccagcac gggcgcgagc agcatcagca acgccgtgat cgcggcgtcc
     5881 acgatgaagc ccagcgtgaa cgcggtgcgc aggtcttcct cggtcagacg ctccttgcgc
     5941 tggaccagcg ccgcgccgat gcccatgtcg gccaggaacg ccgagatcag caccacgaag
     6001 ttcacgatgg cgaacagccc gaaggtctcg ggcgccagga tgcgggccag gatgatgttg
     6061 cccgcgaagg tcatcgcgcg gagcgaaaag gtgcggacca gaaccgcacc gatgccgcgc
     6121 acggcgaggc ggcgaacctc acccgcgctc atcgtgtcct cctcgacgag cgtgcgcgtg
     6181 atgcccgcct cgtcggcggc ctgctcgagc gcagcggcgc cggccgcggt cagaccggct
     6241 tcggcgtcgg gcgtgagccc gagcgggccg acgatgcccg gcgcttcgtc gccgtggctc
     6301 gcggcgtcga tcggatcggg cctacccagc catccgcaca cgcggcgact ccttcccggc
     6361 ggcggcggcg gcgtaggcgg cttcgatgcg atcgacgacg cgctcccagt cgtagcgcgc
     6421 aaccgcgacc tcgcggttgt gcgcgccgat gcgctcggct tcgcggcgca ccagcgtcga
     6481 ggtgagcgca tccgccagca gccgctcgtc gccgggctgc accagacggc cgtgctcgcc
     6541 gtcacgcacg aagccgggca cgccgcccgc ggcggtcgcc acgaccggcc gtcccgacgc
     6601 catcgcctcg aggatggtca tgccgaacgg ctcgccgaag gacggctggc agaacacgcg
     6661 cgcctcgcgg aacagcatcg gcgcgtccgc gggcttgacc gggccgcgca gcgtcacggc
     6721 gtcggcgatt cccagacgct cggcctcttc aaggatctgg cccgccagac cgcgcgggtc
     6781 gtcaccggcc atgatcagcc gcgcgcgagg gatccgctcg cgaaccatcg ggaacgcgcg
     6841 caacagcgtg cggtagccct tgcggggcag cagcacggag tagaacagaa cgaccggatc
     6901 gccgggcagc ggcgagggcg tgaagcgagt ggtgtccacg ccgtagggaa cctcgacgca
     6961 gcggccacgc aggtcgtggt cgatcgcggc gagcgcggga cggcaggtga ccatcagcga
     7021 ggacgcgcgg cgcatcgtgc gcgcgtgcag atgctgctcg tagcgcggca gcgtcaaccg
     7081 cgcgacgccc gagatcaagc ggttgcgcgg cttggccccc ccgtcgcccg cgggccacgg
     7141 aagcatcagc ggcccgacga cgagcggcgc gccggacgcg gtggtgtagc cctcgtcggt
     7201 ggtgagcggc agcagcaggt gatggacgtc ggcctgctcg ctccgggcgg cgcgggccgc
     7261 tcgccgcgcg aacgcgagcc tcgacagcgc ggcgccgggc gcgtgcgcgg agatggtctt
     7321 gaaggtcaca ccggggatct cgtcgtcgta ttcggtgacg taggccgtga cctcgtggcc
     7381 gcggcgcgcc agccgcgaca ggatgttgaa tgcgatcagc gcctcgccgt ggggacggcg
     7441 gtccgagacg atgccggcgc tgacgacgag aatcttcacg cctggatcag ctcccgcgcc
     7501 cacgcgacgg tggcggcgat gccgtcctcc agcgaggtcg ccggctcgta gccgatcgaa
     7561 cgcaggcgct cgatcgaggc gagccacttg tccgggtcgc cggggcggac gctgccggac
     7621 cacacgacct cgggctcggc gttcatcgtc gcggcgacca tggcgagcag ctctccggtc
     7681 gagatgccgc gtccggtggc gacgttgtag acctcaccgc gcagcggccc gccggaggcg
     7741 atgcgcagca gcgcgcgcac cacgtccccg acgtagacga agtcgcggac ctgggtgccg
     7801 tcgccgaacg cctcgatgcg cgacgggctc gccgcgatct tgcgcgcgaa gtcgaagatg
     7861 acctgcttgc gctgacgcgg cccgtagacg ctgaacagac gcgcgctcgc ggtgcgaaga
     7921 ccgtagagct cggcatgcag gcgtaccgac tgctcggcgg tgtacttgtg cagcccgtac
     7981 ggcgagaccg gtgcgacccg gtgatcttcg gtgatcggca gcgactcggg atcgccgtag
     8041 accgcggccg aggaggtgta gacgagcgtg gaggacgggc tgtggcggcg cagggcgtcg
     8101 cagactttga gcgttccttc cgcgacgagc cggaagtcgt agacgggatc ctcgacgctg
     8161 ggtggcacgt aggcggtgcc ggcgaggtgg aagacggtgt cgaagccgcc gcccgccagc
     8221 agcgccggca ggtcggcccc cagcaggtcg ccctcttcga agtgcacgtc gccggccacc
     8281 gcgtcgaggt tggcgatggt tccgccgcgc aggtcgtcga gcacggtgac ggtgtcgccg
     8341 cgcgcgacga gcgcctcgac gagatggctt ccgatgaaac cggctccgcc ggtcacgagc
     8401 acgttcatgc ggacctcttt cggcagatga cctggatgtt ggcgccgtcc ttgacgagcc
     8461 agcgcaccga ctcgacctgc atgcccgaag cccgcgcggc acggcggatc tcgccgatca
     8521 gcacgcgcga gccgtgccag gcggccgaat ccagatcggc gggcggcgga cggcgccggc
     8581 ggcgtgccgc ctcgaccgtg ttgcgcgcga tgcgctcgag ccgcgccagc gggccggcgc
     8641 tcagacggca ggtgcgaagc tggaagccga tcgcgccgcc ggggcgcagc acccggccgg
     8701 cctcacggat gtagcgcagc gtgatcgtgc gatccgggat gtggtggaag acctgcatcg
     8761 agagcacgaa gtcgatgctc gcgtcgggaa gcggcagcgt tccgtcgccg ctggtcgcgc
     8821 tcgcggcaac gccgggatag gacatgagat tgcgcttggc gtgagcgacc atctccggcg
     8881 agacgtcgaa ggcatggacg tggcagaacc gcttggcgag cggacgggtc agtcgtccca
     8941 cgccgcagcc gatctcggcc gcggcttcgc cgccgtcgca cttcgcgtcc gcaaggacgc
     9001 gcgcgacctc atcttctccg gtctgccaga actgctcctc gtcgggtttc gcgtagtcga
     9061 ggatcgtcgt gacgtaccac agcggatttt tttgcgcctt ggtgtcccaa aacgcacgca
     9121 tgttgtcgag cttgctcacc gttcgggcgc cccttcccgc tcggtccgat gttcgattgg
     9181 cgtttcgcgg agggactcac acgagcgacg cgtacagacc ggcgtgttgc gccacgaccg
     9241 tttccggctc gtagtgccgc tgcaccttcg ccgctccggc ccggcccatc cgctcggcgg
     9301 cggccgggtc cttcagcacg gtctcgaccg cctgggcgat cggtcccggc tcgccggcgc
     9361 agacgattcc gtcttcaccg tcgtcgacga ggtctcgcgt gaaccttcct tccgatacaa
     9421 cgaccggagt gccggttcgc cacgcttcca gaacggaaac cggcaggatc tccgataccg
     9481 aaggcagaca taggacattc gcggcgcgca gccaacccca tttttccgcc tcgtccactt
     9541 ttccgcggcg caggattcgg gggtcggtgg cggccgcgaa gtcggcctcg gattcgggcg
     9601 tctcggggcc gacgaacacg acccggacat cgcgggtggc gatgtgcggc agggcgtgga
     9661 gcagggcccg gtacttgtag gggcgcttga cccccaggca cagcacgatc ggcgtgtcga
     9721 tgccgtggac ggcgcgggga tcgaacgagg attcgggggc gggcggcgcg gcgaccggga
     9781 tcacgtgcac ccgctcgggc gcgacgccga gggtgacgta gacgtccgcc tcgccgggaa
     9841 gcaaggccac gaccgcgtcg gcgttgcgat acagcccggc gttgagctcg tcgtcacccc
     9901 agtggccggg atgcgcgaac ggagtgacga ccagcggaag cagccgctgc cgcgcggcgc
     9961 gcatggcggc atgcatgagc ggctcgccgc cccaacagtg caccaggcgc gccgcgaaga
    10021 acgcgcgctt gaaaccgggc gcgagcgcgg tgaccaccag cggcagcgtc gcccggcgga
    10081 tgcggtcgta gccgatgcgg tcggcgccgg gcagcttcga caccgccgac ggcagcatcg
    10141 tggcgcgcgc gcggcggccc gccggcaccg gccaaatctc gacctcgccg tccaggaacg
    10201 gctcgaaccg ctgcgtggcg agcgtggtgt tgagccgcgt gaacggctcg tcgtcgatcc
    10261 gggccgcgaa gatccggagg cgatgccccc ccgccgccag accggtggcg acgcggcgca
    10321 tgtgcgcctc gatcccgccc gagcgcggcg gcgcgctccg cgacacgtag gccaggttca
    10381 gcggcggcgg gtgaggggtt tccggggatt tcggcatctt cgcaagcaga atacgcggcg
    10441 gcggcgatac agtgacggaa cctatgagca cgaacaacgc ctggaatccg agcacgtggc
    10501 gcgatctgcc tgcgctgcag cagcccgagt ggccggacgc ggcggccgtg gacgcggtcg
    10561 ccaaggagct gcacacctgg ccgcccctgg tcttcgcggg cgaggcccga aacctgcagg
    10621 cgcagctcgc gaaggtgcac gccggcgagg ccttcctgct gcaagccggt gactgtgccg
    10681 agtcgttctt cgatcactcg gccgacagcg tgcgggaccg gctcaaggtc ctgctgcaga
    10741 tggccgccgt gctgacctac gcgggcggca cgccgatcgt caaggtcagc cgcatggccg
    10801 gccagttcgc caagccgcgt tcctcaccga tggagaagat cggcgacgcc gagatcccgt
    10861 ccttccgcgg ccacatcgtc aacagcgacg agccaagccc cggcgcccgc attccggatc
    10921 cgcgacggat gctcgaggcc tatcaccacg cggccgccac gctgaatttg ctgcgcgcct
    10981 ttaccgacgg cggcttcgcc gacctcggca aggtgcacca gtggaacaac gagttcgtcg
    11041 ccgcctcgcc gcagggccgc cgctacgagc agatcgccac ccagatcgac cgcgcgctcg
    11101 cgttcatgcg cgcggtcgga atcgacaccg acaacccggc gctgcaccag gtggacgtgt
    11161 ggacctcgca cgaggcgctg ctgctggaat acgaggaggc gctgacgcgg caagactcgc
    11221 tgaccggcga ctggtacgac tgctccggcc acttcctgtg gatcggcgag cgcacccgcg
    11281 accccggcgg cgcgcacgtc acgttcctcg ccggcgtagg caacccgatc gggtgcaaga
    11341 tcggcccggc cgccgatccc gccgaggtga tcgagctgtg ccgccggctc aacccggatc
    11401 gcatcccggg acggctcacg ctgatcaccc gcatgggcgc ggcgaaggtg cgcgaggcgc
    11461 tgccgccgat cctggaggcc gtgcgcgacg cgggcgagcc ggtgatctgg tcgtgcgacc
    11521 cgatgcacgg caacaccttc acgagcggta ccgggctcaa gacccgccac ctcgacgaca
    11581 tcgtcgccga gatcgacgga ttcttcgccg cgcaccgcga gacgggaacg cgcgcaggcg
    11641 gcatccatct ggagatcacc ggcgcggccg tgaccgagtg cctcggcggc ggcgacaacc
    11701 tcaccgaggc ggacctcgat cgcgagtaca cgacgctgtg cgatccgcgc ctgaacgcca
    11761 agcagtcgct ggacctggcg ttccacctcg ccgacctgat gacgcagaac ggcgcctgac
    11821 gggcgacgaa ggcggcgaac acgatgccgc ccggccggct tcgatgcgcg aagcgccgga
    11881 cccttcgcga tcagggtccg gcgcttcgaa cgaaaccgat cagcgggtga tcgtgatgtc
    11941 cgactccgcg agcgccagcg attccgcgag cgtcatcggc atcggcttgt agcgccactg
    12001 cacgtacagg tcgaactggt cacgcgagtg acgcgcctcc tgaccgagca ggttgatgaa
    12061 gccgctctgg ccgggcgaga tcacgctgcc gtgctcggcc cgcgactcgc ccaggatgcc
    12121 cgagccgctc ggcgggtcga ggtagagcac cagatggttg taggtaccgc ggttcatccg
    12181 gatgtggttc ggcacgttgc cgggcggcac gttgaccgcc cccggctgga gcgcccccac
    12241 cgtgacgccg gtgttggagc gaaccgtgtt gcgtccggtg tcgcgcgcga cgctggtgag
    12301 caggtcggcg ttgaggcgcg tgtagttctg ctcgggctgg tccgacagcc aggtgcgcgg
    12361 ctcgctgttg ccgaactgcg cggccagcgc ggtgaccgca gacgacagcg cgcgtgcggc
    12421 gacctgctgg cgcgtctcgc tcccgagcca gttgcgctgc ggctgcagcg cggcggccgg
    12481 gttcatcacg tgcacgagcg tgctggtgct ggtgcggttc ggcacgccga cgaggtcgtc
    12541 gttgaagacc gcggcgcgca ggtcggacac gaaccggccg aggatcgtgt agccggccga
    12601 gtccaccttc ttgtcgccgt tcgcgtcgta gatgaagccg ttgtagctgc ggagcacctc
    12661 gagcgccttc tgctcgacgg cattcggcga ggatgccggc acgtactgcg tgaagtagtc
    12721 ggcgttgaag tcgcggaagg cggcatagcg aatgttggcg ttgaggtcct gccagtccac
    12781 ctggcggtcc gggttgaccg aaccgcccca cgggtcggga cgggccgggc cggcggcgtc
    12841 gaggttgtcc aggaacgtct gcaccccgtg aaccgctccc cagccgttct cgcggtccca
    12901 gtccaccgac ggcttgttgt tccagttcgc gatccagccg cgcggcaggt tgatcgcgtg
    12961 cggcacctcg ttcgcggtca gcagcccctg ccattcctgc gttcctgtgc cgacgaacgg
    13021 cagacgaagg tcgccggcgg cgcggcgcgg gaagttgccc ggatgccagt agccgatgtt
    13081 gccctgatcg tcggcgtaga acatgttgtg gttcgacgcc agcatgttcg cgtaggtggc
    13141 gaagtcctcg agcgagcgca cctggttgta gcggaagaac ccttcgatcg tgccgccctc
    13201 gcggttgaac cacgagtagc ggacggcata ggcgacgttg ttggcggtgt cgaacgacag
    13261 caccggtccg tgccgcgtgc ggcagatctc ctgctggtcg aacggaacgc ccttctgcgc
    13321 gtagacctcg gtgcggcact ccatgttctc ccacaccgcg ggctgcgggc cggcgccgtc
    13381 cacgtcgaac aggtactgct tggcgttgct cgggttgagc tgaaccacga aggtgtcggt
    13441 gatgtcgctc gcgccggtcg tggtcgtcca gttgtagccg gtgccgcgtc cgatcagcac
    13501 tgccggaccg accccggcga aggtcatgcc ggtcgcgtcg atctggccgt ccgcggagtg
    13561 catgccgaag tcgctgagcg cctgcggcag gaagtagccg acctgcgggc cggcctgcag
    13621 cgcggtgttg ccgctcgtgg tgcggtgcgg cgccacgatc tgcgcgttgg agccgaactt
    13681 gaaaaagatc ttctcgagcg tctgacggcc gtcttcgagc acgtcgacgt cggcgagcac
    13741 cgagccggcc accgggatgc gcagcaggtc tgcctgggac aggatcggcg tgacgccttg
    13801 cgccggtccg gccacccgca tggcttgcgg caggctgatg cgcgcgtcgc cttcgagcgt
    13861 cttgaggtcg gcctcggggc gcgcgcgcga cggcgtggat ttccagtcgt agtcggccgg
    13921 gatcgacagc ggcgcgtcgg ggtcgttgat ccagatgagg tcgtcccaga tcttgcgtcc
    13981 ctcggcctgg ccgaatttgg cgatgtaggc ctggaggtcg gcgaggttgc ggacctcgct
    14041 tcccccgccc gagccgaacg tgtagatcag gtagtcggcc atcgccatcg agtcgtcgat
    14101 ggtccactcg tcgagcggct cgccgaactc ggcgaactcg aacggcatct tggcggcggg
    14161 ctcggccttg gcctcggcga tccacttgtt gatgccgtcc acccacgcga gccaccagcg
    14221 cttgatgtcc ggcgggtagg tcgcgaaccg ggcggcgcgc tcgctcgcgg tgtaggtcca
    14281 gaagcgcacc gtcttgtcgg cgccgacgat cagcgacccc agcaggtgag acaggttgcc
    14341 tttcgagacg aggcggaaga tgtgcatctg ccacagccgg tcctgcgcga gcgcgtagcc
    14401 ggcaccgaac gccacgtcct cggcggtcgc gccgtagacg tgcggcacgc cgaactggtc
    14461 gcgaacgatc cggatctggc tcgcggcctt cgccgggaag ccgacggccg acagcgcgag
    14521 cagcagcgcc gcaacgatga atggtctgat gcgagccctc atcctgtccc ccctggatgc
    14581 gatggagccg gacgacaccg gcttagggat ttgtcctgat tgtccccgat gcgcgggcga
    14641 gggtcaaggt cgctgctcgg gcgccgtacg cccgcggaag ggtgtgcgaa cggtcatcgc
    14701 cggcggccgg tccacacgaa cggcaggaac aggataaaga ccggcacgaa cgtgcgcagg
    14761 acgagcgaaa cgacgacgct gatcgcgatg ccggccagca gcatcaccag cggtccggtc
    14821 cggcgggacc gatcgcgaac cggcggaggc gtttgcatcg ccccattacc tgccccgtcg
    14881 gcgcgggagc catgctagat gcgcgggtcc aaggcgcgga agcggatcat gccggccaca
    14941 acccagccgg tctgcaccag cggcacgacc gccgcgcgga tcagcgcgcc gggcgtgcgc
    15001 gcccgcagaa cgtgcgcgac gcggcgcagc gtccaggcgg ccagcacgcc cgtcgggacg
    15061 gcaacccacc aggcaccggt cgcgagcgcc acgacgaacg cggcgagcgc gagcacgtag
    15121 tccacgtacg gcgcggggaa ggccggcacg accggcttga gcagccgccg gtacgacgct
    15181 ggatgcttct tgtgcagcag cgggttgtag aagcgcttgc gcgcctcgcg ccagaacttg
    15241 acggtgcctt cctggagcac caggtggtgc gcgagcacgt cggccacgaa ctcgatccgg
    15301 ccgccccgtt ccagcacgcc gaaggcgacg tcggagtctt cgaggaacgc catgcggaag
    15361 cgctcgtcga agccgccggc gatcgcgtcg cggcggtaga tcatgttgca ggtcatgtag
    15421 agctggccgc gctcgttctc catctggtgc gtgtaggttc ccttcacgcc tttcggcagc
    15481 gtgcgccctt cgacgccggc aagggccgca tcacgctcca ggcgggcgag cgcgcgctcg
    15541 agccagtcgg acgcgacctc gacgtcggcg tcggtgaagg cgacgtaggt cgaatcggtc
    15601 gcggccaggc ccgcgttgcg tgccgcggcc gggccggcgt gctcccgctc gatcagcgtg
    15661 accgaggcgc gcaggttcgc ggccaggtgc gccacgacgc gctgcgtgcc gtcggtcgag
    15721 ccgtcgtcga ccacgacgat ccggaactcg cgccggtcga gcgtctgcgc ggcgagcgcg
    15781 cgcagacacc cttcgatcag gtgcgcgccg ttgtagacgg gcacgatcac cgcgacgcgg
    15841 ctgaacgccg gagcggaagg ggcagcggac gaagcaccga gtgggcgctc ggtaaaagtg
    15901 cttcggctca cgccgcctcc ttccagctcc ggcgagttcg ctcgtagatc tcaacgaccc
    15961 gcgccacgtg cgcgtcgagg gcgaaatccg cttcgatgcg gcgctttgcg gccgcgccca
    16021 tgcgctcgcg cagcgtcgcg tcggccagca gccgcgcgat cgcacggccg aacgcggtcg
    16081 cgtcgcgcgg cggcacggcc aggccggtcg ctccgtcgtc gagccactcg cggatgccgc
    16141 cgatgtcgct cgccaccacg ggcacaccca cgctcatggc ctcgagcccg gcgatgccga
    16201 atggctccgg ccacgcgctc ggcacggcgg cgacggtcgc ggcgcgcagc cagcccgcga
    16261 gcgcatcgcg ctgcgtccag ccgagaaagg tgacgcggtg acgcggcggc acggccgcgg
    16321 cggcacgccg cagcgcgggc agatgatggc cgtccccggc gatgaccagg tgcgccggca
    16381 tctcgacggc gtgcatcgct tcgagcagga gatcgaagcc tttctctttg gtggcccggc
    16441 cgtgtgcgag cacgaccggg cgcgcgtcgc ccggctcggc gggtggggcg gcaggcagcg
    16501 cggtcgccgg atacggggtg acctctacgg gcgcctcgat gccgacgcgg ccgagcgcgt
    16561 cgcgcatcgc gccgctgaac acctggatgc gcggcccgcg ccgcgcggcg gcgtgcagac
    16621 gcttccagcg caggtacggc tcgagggtcg cgttggcgcg caggctgccg cagtgcgcgg
    16681 cgacgttgta tccaaggcac gtcgcgcccg gcgtgacgtc gcacgcgcgg ttccacgccg
    16741 cccagtagcg cgtgccggta ggacagtcga gccggtggtc gtgcacggcg ctcaacacgg
    16801 gagcgatctc gtcgcacagg ccggcgagcc aggctgcttc ggcgttgtgg acgtggatga
    16861 cgtcgggcgc gaaggcctcg acggcacgcc gcacctcggc ggcctcgacc ggagccgggc
    16921 tcgcgttgcg tgatgtgatc gcggcgatct gcgttcccgc gcgcacctcg agccgcgtat
    16981 cgtcgggatc gaaccggtac gcgagtgcga cctcatggcc ggcgttgcga agcccggcgc
    17041 acagctcgcg cacgtaggac tcggcgccgc ccttgacgga gccgtaccgg ccgatctgaa
    17101 ggactctcac ggcttgcgcg cggtcaccag gagctcgtgt ccgatgtgca cgccggttgt
    17161 ggcgagcacc gcgcgcgcga ccgtgacgta ggcgcgcttg aaacgatcca tgcgagcggt
    17221 gtactgccgc tccgggatca tgcagccgac ctcgaccttg ccgaagccga tcccgcgcag
    17281 caggccgcgc accgacgact gtgagaagtg gttgacgtga cccagaccgc cgatcacgac
    17341 gcccggtccc ttcttcagcc gccgcttgag attctccttc gggatctgga tgctgccgtt
    17401 cggcaccgtc atgacgagca tgccgcccgg cttgaggatg cgaaagatct cttcgaaggt
    17461 ctctttgggg tcgatgacgt gctcggcgac gtcggtcatg gtgacggcgt cgaagaagtc
    17521 cggctcgaac ccggccttgc gcagctcggt ggtaaagacg ctgtagccga ggtcttcgcg
    17581 agcgtatccg gaggcgactt tgttgatctc gacaccccac ggctcccagc cgcgcttggc
    17641 cgcgacctcg agcagcgtgc cggtgaagca gccgacgtcg aggatcttgc cgcgccgcgg
    17701 gtgcttggcc tgaagccggt cgatcagatg ctcgaacatg cgcacccgcg cctgctgccc
    17761 gttgaggttg aagtcgatgt cgctcatgtg ctggtagcgc tcgtcgagca catcctgggt
    17821 cggacgcggg ttcgcgtaca ggaagccgca gtgcttgcag cgcacgatcc tcatctcgag
    17881 atcttcgggg aagcggccgg tgagcggcga cgacagctcg cccatcggct cggtgtgaac
    17941 caccttgccg ccggtcttgg cgcagatcgg gcacggcgcc cagtacaggc cctcggggag
    18001 cgtgcctctg gtctttgtcg tcatcggtcc tccagtcgag cgaagaattc gtgcaaatcc
    18061 ttggcgacgg cctccggcgt gaagcgcgcg cgcaccgcag cgcgcccggc gtcgccgagg
    18121 cgccggcgcc gctggtcgtc gtgggcgagg gccgccacgg cggccgcaag gccctgcggg
    18181 tcgccaccgg cgaggacgat gcccccgccg gtggcacgca cggtgtcgcc gagcgcgccg
    18241 cattcggtgg caacaaccgg aacgccggcg gcgttggcct cgatcaccac gcggccgaac
    18301 tgctccttcc atccgcgcgt cgtccgcgac gggatcacga gcacgtcgac ggcggcgaag
    18361 acctgtgcga ccgcgtcgtg agcaacgctt ccgaggaagc gggcgtcgag cccgaggcgc
    18421 gtcgcctccg cttccgcgcg tgcccgcagc ggcccgtcgc cggcgacgac cacacagcct
    18481 gctttcgcgg cggccgcagc gcggaggaag accatgacgc ccttctcctc cacgagccgg
    18541 ccgacgaagc cggcgaccgg gcgcgggagc cccggcagca tcggcgatgc ggcatcgaag
    18601 cgcgacggat cgatgacgtt cgggatcgaa agcatcgggc cggtgaagcc ggacgcgcgc
    18661 agcacgcgtg ccgcaccctc gagacggacc gtcgcaccgg ccgcctcgcg cagcacccgg
    18721 cggcggatcg actcgaaggg cggcggaaga cgcttggcca cgttctggga ctggtggaag
    18781 gcgaacggca caccgagctc gcgcgcgatg cggtcgacct gcatcgcggt gaagctccac
    18841 ggctcctcct cgacgtagat cagcgtcggc tcctggccgc ggatcgcctc gcgcaagccc
    18901 gcgtacagga tcatgtgcac gccggcctgc cggacgccgc gacggaatcc gccgatgcgc
    18961 acgggaagcg ccgtcgcgtc gacgccgtcg agcgcgcgga acgggatggt gcctcgaatg
    19021 tcggtgcgca gcgccttcgg cgcgatgacg tgcacacgca cgccgcgtcc ggcgagcgcg
    19081 cggaacggct cctggttgac gtccacgacg caggcgtggc tgaccaccac gacggacgac
    19141 tgcttgacgc cggcgcggct catacgcgcg ccccctcgcc ggcgagcgcg gcctcgaccg
    19201 cgcggacggt gtcctgccag cggtagcgcc gctcgaccag cgcacgcccg tttgcgccca
    19261 gccgggcgcg cagcgtcgcg tcgccgagca cgcggcgcac cgcgtcggcg aaggcgcggg
    19321 gatcgtcggc gagcagcagg tcgcggtcgt gctggacctc gagcccttcg gcgccgatac
    19381 gcgtcgagat cacggccttg cccatcgcga gcgcctcgag aattttcagg cgcgtgccgc
    19441 tcccggaccg aagcggcacg accacggcgc cggcgcgggc catcatgtga cgcacgtcgg
    19501 tgacgcggcc ggcgaggccg acgccttgcg gcgcggcgaa gccggcgggc tcgccggcga
    19561 cggtgagcgt gaccccgccc aggtgcggca ggatctcgtg cgcgaaccag ttgatcgcgt
    19621 cgcggttcgg cgggaacgac agcggaccga cgaacagcag ctggcggtcg ttgatcgcgc
    19681 cgccgcgtgg agcgaacagg gcggtatcca cgccattggg cgcgagcacg aacgggcggc
    19741 ccgtcagcgc ctccagcgcg gcccggtcgc cggacgagac gcaggttcgg gcgtccacgc
    19801 tctgcgccac gcgccgctcc agccggacca tgcgcgcgaa ctcgcgtcgc gcgtgcagcc
    19861 ggtgcggcag accgcggtcg cgctcggtct ggcggcggat gttggtggac acgacatcga
    19921 tccaacccat catcgtgcgc gccgacggta cggcctcggc gcaggcgcgc aggcagggct
    19981 ccatctcgat cggctcggcg tagatcgcgt cgggccgcac ctcgcgcgcg aacgcggcgg
    20041 cggcggcgag cgtgtcgccg tcgaaggctt gccgcacggc cttgggaaga ggatcggtga
    20101 gcagctgcgt ccagaaccgg atgcggccac gctcgggaag gcccgggtac ggcacggttc
    20161 ggacgacggc gagcggcgcg tcggccgtcg cctcggcgga gccgccttcg ccgtggaggg
    20221 cgaacagcga gatctcgtgg gaggccgcga ggccctccaa aatcgcggcg atgcggcgcg
    20281 tgccgccggt atgcggcggc cagggcagcg cgggcgtgag cgcgaggatc ctcatcgcgt
    20341 tcccaccgcc tcgcgccgcg cgatcacgtc gccgaggatc ttttcgaccc ggtcgaggat
    20401 gcgctcccac gacaggtggg ccacgctcgc gcggcactcg tcccgcgaag ccggaagcgc
    20461 aaggccgcga acgaccgcgc cggcgagcgc tgcgggatcg cggacggcgt cgagcaggat
    20521 gccggtctcg ccgtcgcgca cgtgctcggc gacgccggcg ctgcggccgc acacgaccgg
    20581 cgtgccgcac gagagcgatt ccaccgccac cagaccccac gggtcgtagc gcgtcgggaa
    20641 cgcgaacagg tcggcggctg cgtaggcctc ggacggctcg gcgatccggc cgacggtgcg
    20701 aacgtcctcg ccggaaagtc cggcctcgcg cgcgagccgc gcggcttcgg cgccgcccgc
    20761 aggcccgccg atcaccagca gcgcaccggg gaccgcggct tgaacgcgcg ggaacgcggc
    20821 gatgagcgtg tcgatgccct tgcgcgcgat ctcgttggca agcgtgagca cgacgggccg
    20881 ctcgcccgct ccccattcgg cacgaacacg ctcgcgcacc gcgggatcga atcggaactc
    20941 gtccatgttg acgccgttgg cgagcacacc gacgcggccg tcgggaacgc cgtacaggcg
    21001 tgcgtagtcg tcggcgctgc gttgcgaggt cgcgtagagc atctcgaagc ggccaccgcc
    21061 gacgatgcgc ttctcgacgg ccatcaccac gcgatggtgg gggttcaagc ggcggcgcag
    21121 cccttcgcgc gtccagggcg gcgcttcggc tctgccttgc tcgaaccagg cgcgatgcgg
    21181 cccggcggtg cccatgacgg tcggcgcgtc cacggcggcg ccgaacgagc aggcgacgtc
    21241 caccggatgg gcgcgaagcg cggcggccgc ggcgcgcggg aaggtcgcgg cgcgtcgcca
    21301 gttcggcgag cgcgctcccc cgaccggcac gtaggtcagg ccgggatcgc gctcgatcac
    21361 ctcctgcgcg aagacgacga cgtcgtggcc gcggcgatga aggccgcgcg cccactcgac
    21421 ggcgacgcgc tcgagcccgc cggtgcggcg gtagacggag atgcagatgc cgaccttcat
    21481 acgcgctccc ggacccggcg cggggcgtag cgttccagga cggcggtgaa ctgttcgacc
    21541 atgcgcccgg cggtgaacgc ggcgcgggcg cgagcccggc cggcgttcga gagccgcacg
    21601 gtgagcgcgg ggtcggcgag gatctcggcg acgcggcccg cgatctgctc gtcgtcgccg
    21661 cattcgacga ggaagccgtc ctcgccgtcg gtgacgatct cggcgacgcc accgccgcgg
    21721 gtggcgacga ccggaacgcc ggcggcgagc gcctcgacga tgacgatgcc gaacggctcg
    21781 gcgaccgagg cgtgcacgaa cacgtcgagc gcggcgagca cggccggcac gtcgtcgcat
    21841 gcgccggtga agatcacgcg tccggcgatg cccagctcgt cggcgagcgc gcgcaggccg
    21901 gccgggtagc cggcggagaa cccgccgagc gcgtccccgg cgacgacgaa gcgtgcatcc
    21961 ggatgcgcgg cggtgagccg ggcggccgcg cgcaggaaca catgctgtcc cttccac
//
LOCUS       DATLFO010000038         5825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_90882, whole genome shotgun sequence.
ACCESSION   DATLFO010000038 DATLFO010000000
VERSION     DATLFO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90882"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..748
                     /gene="pyrH"
                     /locus_tag="VM841_01325"
     CDS             23..748
                     /gene="pyrH"
                     /locus_tag="VM841_01325"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVL88852.1"
                     /translation="MAARGPYRRVLLKLSGESFAESDSKLGIDPRIVNSVADQLKDVV
                     NDLGIQVAVVVGGGNIFRGTSTKAEGMDRSRADYMGMLGTVINSLALQDALEKRGVQT
                     RVQSAIQMTQVAEPYVPRRAVRHLEKGRIVIFAAGMGTPYFSTDTTAAQRALEVGGEA
                     ILKGTRVDGVYDSDPEKNPEAKRFDSLTYQEVLQRGLGVMDATAIALAMDNKLPIVVF
                     NHEVAGNIRRVLSGEPIGTLVHG"
     gene            822..1379
                     /gene="frr"
                     /locus_tag="VM841_01330"
     CDS             822..1379
                     /gene="frr"
                     /locus_tag="VM841_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVL88853.1"
                     /translation="MIDQAMKQADEKMAKAVEVTREEFAAVRTGRASPALVQRITVDY
                     YGSPTPLQQLASVSVPESKSLLIHPYDRNAVSSIEKAIMASDLGITPSNDGANIRLNF
                     PALTEERRKELIKVVRDRAEHGRVAIRNIRRHTKDEIDKDAKEGKVSEDDARRAEKDL
                     QKLTDKHVGEIDSMLQRKEQELMEV"
     gene            1401..2252
                     /locus_tag="VM841_01335"
     CDS             1401..2252
                     /locus_tag="VM841_01335"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVL88854.1"
                     /translation="MSAQPAAAGEPEPAPSAKGRNLPIAIVTGLSLAGLIFGTLFTSR
                     IAWEIVVIVVVTYGLSEFYAAVQSRGYRPATWLGLAASVAMMAGASWRGPRAVTFVLA
                     MFLVVAFLWYLADPERRNVLANISVSVFGLVYTGVMGAHVILMRELPHGPGITIAFIG
                     LVAFYDIGAFASGSLLGKRKLAPSISPGKTWEGAIGASVLILALGAGLGPLIEPWSLW
                     SGLALAGATAVVAPLGDLAESVLKRDLQIKDFGQLLPGHGGMLDRIDALLLMAPVAYW
                     LARWLTI"
     gene            2294..3421
                     /gene="dxr"
                     /locus_tag="VM841_01340"
     CDS             2294..3421
                     /gene="dxr"
                     /locus_tag="VM841_01340"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVL88855.1"
                     /translation="MGPVKRIAILGSTGSVGTQALDVVRKHPDRFRVVALVAGRNAEL
                     LSQQVDEFRPRLAALAAAEGGNGAFARGPEAVLEAASLAEADVVVNALVGSRGLVPTI
                     TALEHGKTVALANKESLVAGGEIVMRLVRDHPERLLPVDSEHSAIFQCLAGNRLGDVR
                     RVILTASGGPFRGRDATSLRGVTPDEALNHPTWSMGKRITIDSATMVNKGLEAIEAHH
                     LFRIPIDRIEIVVHPQSIVHGIVEYADGSSIVQASVPDMRLPLQVALAWPERLEGGAE
                     PLAWPTLGSLQFEPLDAEVFPAPRLAMEAARRGATYPCVFNAADEEAVAAFLDGRLDF
                     PGIVRVIEQVLSRHDAAGPVTLESVLDAEEWARSEARSIIE"
     gene            3418..4506
                     /locus_tag="VM841_01345"
     CDS             3418..4506
                     /locus_tag="VM841_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HVL88856.1"
                     /translation="MKALLFVFLILVAIGLHEFGHFITAKKFGMKVDRFFIGFGPPIW
                     SVKRGETEYGLAWIPAGGYVRIAGMNPFEEVPEEDRGRIFKAKKAWQRAIVLVAGSTM
                     HFIIAFVMLAAMLMTAGVPDGDATRTIHEVEPETPAAAAGFEPGDTIVAVEGTRVQTW
                     DEAREIIRSKPGETVEFTVARDDGETTLRATLQSSNSRGEQVGFLGVAPDLETVTRPP
                     ATAVVESAKFIPYASWESLKALGKIVSPSSFAHMFDVLRGKEQRTLEDPASIVGVTRA
                     AGQSDMINLLYLFIGFNIFIGVANLLPLPPLDGGHLAVLAYEKIRKREVDMRKLIPVS
                     ATVIALFVGLFVLSLAMDIVAPLPALPN"
     gene            4506..5681
                     /gene="ispG"
                     /locus_tag="VM841_01350"
                     /gene_synonym="gcpE"
     CDS             4506..5681
                     /gene="ispG"
                     /locus_tag="VM841_01350"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVL88857.1"
                     /translation="MSIPRRQSRAIRVGSVPVGGGAPVSVQSMTITKTADYNSTLQQI
                     AELAAAGCDIVRVAVPDRDDAGALDKIASHSTIPVIADIHFQWKYAIMALEAGCHGIR
                     INPGNVKKHDKVALIGREAAARGAPIRIGVNAGSLEAPVLERFGGPTPEAMVFSALEE
                     VKLLEDVDFRDIKISVKHNDPWTMIQAYRMLASQCDYPLHLGVTEAGTPARGVYKSAI
                     GIGTLLAEGIGDTIRVSLTAPPVEEVKAGIAILETLNLRERGVDLVACPSCGRAEVDV
                     YALAGAVEKRMSEIDVPMRIAVMGCVVNGPGEAREADVGVAAGKGKGQIFVRGEVLRT
                     VPEDDIVDELFKEAHRLADELRAERGDGTPAVVSVAPQLQSLMQIGKGPSKLERTQG"
BASE COUNT          938 a         1940 c         2021 g          926 t
ORIGIN      
        1 cttccgcgtc gggggagagt agatggcggc gcgcggcccg taccggcgcg tattgctcaa
       61 gctttccggt gagtcgttcg ccgagtcgga ctccaagctc ggcatcgacc cgcggatcgt
      121 gaactcggtc gccgaccagc tcaaagacgt cgtcaacgat ctcgggatcc aggttgcggt
      181 cgtcgtcggc ggtggcaaca tctttcgcgg gacctcgacc aaggccgaag gcatggaccg
      241 ctcgcgcgcg gactacatgg ggatgctcgg cacggtgatc aactccctgg ccctgcagga
      301 cgcgctcgaa aagcgcggcg tgcagacccg cgtccagtcg gcgatccaga tgacccaggt
      361 cgcggagccg tacgtgccgc gccgcgccgt gcgccacctc gagaaggggc ggatcgtgat
      421 cttcgcggcc ggcatgggca cgccgtactt ttcgaccgac accaccgccg cgcagcgggc
      481 cctggaggtc gggggcgagg cgatcctcaa gggaacccgc gtggacggcg tctacgattc
      541 cgatcccgag aagaaccctg aggcgaaacg gttcgactcg ctcacctacc aagaggttct
      601 gcagcgcgga ttgggtgtga tggacgcgac ggcgatcgcg ctggccatgg acaacaagct
      661 gcccatcgtg gtcttcaatc acgaggtcgc ggggaatatt cggcgcgtgc tgtccggcga
      721 gccgatcggc acgctcgtcc acggctgaca cgcgcggccc gtgcggccct cgcgcgggct
      781 cggatagagt tccggcatcg caacggaccc aggagaggcg aatgatcgac caggccatga
      841 agcaggcgga cgagaagatg gccaaggccg tcgaggtcac ccgggaagag ttcgcagccg
      901 tgcggaccgg ccgcgcctcg cccgccctcg tgcagcgcat caccgtggac tactacggct
      961 cgcccacgcc gctgcagcag ctcgcgtcgg tgagcgttcc cgagtcgaag tctctgttga
     1021 tccaccccta cgacaggaac gctgtctcct cgatcgagaa ggcgatcatg gcgtccgacc
     1081 tggggatcac gccctccaac gacggagcca acattcgcct gaacttcccg gcgctcaccg
     1141 aggagcggcg caaggagctc atcaaggtgg tgcgggaccg ggccgagcac ggccgcgtcg
     1201 cgatccgcaa catccgtcgc cacaccaagg acgagatcga caaggacgcc aaggagggca
     1261 aggtctccga ggacgacgcc cgtcgcgccg agaaggactt gcagaagctc accgacaagc
     1321 acgtcggcga gatcgactcg atgctccagc gcaaggagca agagctgatg gaggtctagc
     1381 cgcacggcgg cgacgacccg atgagcgcgc aacccgcggc ggccggcgaa cccgagccgg
     1441 cgccgtccgc gaagggacgc aacctgccca tcgccatcgt gaccggcctg agcctggccg
     1501 gcctgatctt cggcacgctg ttcaccagcc ggatcgcatg ggagatcgtg gtgatcgtcg
     1561 tggtcaccta cgggctctcc gagttctacg ccgcagtgca aagccgcggc taccgtccgg
     1621 cgacctggct cggcctcgcc gcctcggtgg cgatgatggc cggtgcgtcg tggcgcggcc
     1681 cgcgggccgt aacgttcgtg ctggcgatgt tcctcgtcgt tgcgttcctg tggtatctcg
     1741 ccgacccgga acggcgcaac gtgctcgcga acatctcggt atcggtattc gggctcgtct
     1801 atacgggcgt gatgggcgcg cacgtgatcc tgatgcgcga gcttccgcac ggccccggca
     1861 tcaccatcgc attcatcggg ctcgtcgcct tctacgacat cggcgcgttc gcgtccgggt
     1921 cgctgctggg caagcgcaag ctggcgccct cgatctcgcc cggaaagacg tgggagggcg
     1981 cgatcggcgc gtcggtgctg atcctggcgc tgggagcggg gctcggcccg ctcatcgaac
     2041 cgtggagcct gtggagcggc ctggcgctgg ccggggcgac ggcggtggtc gcgccgctag
     2101 gtgatctggc cgaatcggtt ctcaagcgtg acctgcagat caaggacttc ggccagctcc
     2161 tccccgggca cggcggcatg ctcgatcgca tcgacgcgct gctgctgatg gcgccggtcg
     2221 cctattggct ggcccgttgg ctcaccattt gaccgatctt cgccgccagc gaaggccctc
     2281 gggcgctggc agaatgggcc ccgtgaagcg aatcgcgatc ctcggatcga ccggctccgt
     2341 cggaacccag gccctcgacg tggtgcgcaa gcaccctgat cggttccggg tcgtggcgct
     2401 ggtcgccggc cgcaacgccg agcttctgtc ccagcaggtg gacgagttcc gcccgcgtct
     2461 ggcggccctt gccgcggccg agggtggcaa cggcgccttc gctcgcggac ccgaagcggt
     2521 gctcgaggcc gcatcgctcg ccgaggccga cgtcgtggtc aacgcgctcg tcggctcgcg
     2581 cgggctcgtc ccgacgatca ccgcgctcga gcacggcaag accgtcgcgc tggccaacaa
     2641 ggagtcgctc gtcgccggag gcgagatcgt catgcggctc gtacgcgacc atcccgagcg
     2701 gctgctgccg gtggactcgg agcattccgc gatcttccaa tgccttgcgg gcaaccggct
     2761 cggcgacgtg cggcgcgtga tcctgaccgc cagcggcggc ccgttccgcg gccgcgacgc
     2821 gacatcgctg cgcggcgtta ccccggacga ggcgctcaat cacccgacct ggtcgatggg
     2881 caagcgcatc acgatcgact cggccaccat ggtcaacaag ggcctggagg cgatcgaagc
     2941 gcatcacctg tttcgcattc cgatcgaccg gatcgagatc gtcgtgcacc cgcagtcgat
     3001 cgtgcacggc atcgtggagt acgcggacgg ctcgtcgatc gttcaggcgt cggtgcccga
     3061 catgcggctg ccgctgcagg tcgcgctggc gtggcccgag cggctcgagg gcggcgccga
     3121 gccgctggcg tggccgacgc tcggctcgct gcagttcgag ccgctcgatg ccgaggtgtt
     3181 tccggcgccg cggctggcga tggaagccgc gcgacgcggt gcgacctacc cctgcgtgtt
     3241 caatgctgcc gacgaagaag cggtcgccgc gtttctggac ggccgcctgg acttccccgg
     3301 catcgtccgg gtcatcgaac aggttctgtc tcgtcacgat gccgccgggc ccgtgacgct
     3361 ggagtcggtg ctcgacgccg aagaatgggc ccggtccgaa gcgaggtcga tcatcgagtg
     3421 aaggcactgc tgttcgtatt cctgatcctg gtcgcgatcg gcctgcacga gttcggccac
     3481 ttcatcacgg ccaagaagtt cgggatgaag gtggatcgct tcttcatcgg gttcggaccc
     3541 ccgatatggt cggtcaagcg cggcgagacc gaatatggcc tggcgtggat ccccgcgggc
     3601 ggttacgtgc gcatcgccgg catgaacccg ttcgaggagg ttcccgagga agaccggggc
     3661 cggatcttca aggcgaagaa ggcatggcag cgggcgatcg tgctcgtggc gggctcgacg
     3721 atgcacttca tcatcgcctt cgtgatgctc gcggcgatgc tgatgacggc gggagtgccg
     3781 gacggggatg ccacgcgcac gatccacgag gtcgagccgg aaacgccggc ggcggccgcg
     3841 ggcttcgaac ccggcgatac gatcgtcgcc gtcgaaggaa cgcgcgtgca gacctgggac
     3901 gaggcccgcg agatcattcg gtccaagccg ggcgagaccg tcgagttcac ggtggcccgc
     3961 gacgacggcg agaccacgct gcgcgcgacg ctgcagtcgt cgaactcgcg cggggagcag
     4021 gtcggcttcc tcggcgtggc tcccgatctg gagacggtga cgcgtccccc tgccaccgcg
     4081 gtggtcgaga gcgcgaagtt cattccctac gcgtcgtggg agagcctcaa ggcgctcggt
     4141 aagatcgttt ctccgtcctc gttcgcgcac atgttcgacg tgctgcgggg caaggagcag
     4201 cgcacactcg aggacccggc gtccatcgtc ggcgtgacgc gggccgccgg ccagtccgac
     4261 atgatcaacc tgctctacct gttcatcggg ttcaacatct tcatcggtgt ggcgaacctg
     4321 ctcccgctgc cgccgctcga cggcggccat ctcgcggtgc tcgcctacga gaagatccgc
     4381 aagcgcgaag tggacatgcg caagctgatc ccggtgagcg caaccgtgat cgcgctcttc
     4441 gtcgggttgt tcgtgctatc gctcgccatg gatatcgtcg cgccgctgcc ggcactgccc
     4501 aactgatgtc catcccccgg aggcaatcgc gcgcgatccg cgtcggctcg gttccggtcg
     4561 gcgggggagc gccggtcagc gtgcagtcga tgacgatcac caagacggcc gactacaact
     4621 cgacgctgca gcagatcgcc gagctcgcgg ccgccggctg cgacatcgtg cgcgtcgcgg
     4681 tgcctgatcg cgacgacgcc ggggcgctcg acaagatcgc gtcgcattcc acgatcccgg
     4741 tgatcgccga catccacttc caatggaagt acgcgatcat ggcgctggag gccggctgcc
     4801 acggaattcg catcaatccc ggcaacgtca agaagcatga caaggtggcg ctgatcggcc
     4861 gggaggcggc cgcgcggggg gcgccgatcc gcatcggcgt caacgcgggc tcgctggagg
     4921 cgccggtgct cgagcgattc ggcggcccga cgccggaggc gatggtgttc tcggcgctcg
     4981 aggaagtgaa gctgctcgag gacgtcgact tccgcgacat caagatctcg gtcaagcaca
     5041 acgacccgtg gacgatgatc caggcgtacc gcatgctcgc gtcgcagtgc gactacccgc
     5101 tgcatctggg cgtcaccgaa gcgggcacgc ccgcgcgcgg tgtgtacaag agcgcgatcg
     5161 gcatcggcac gctgctggcc gaagggatcg gcgacacgat ccgcgtttcg ctgacggcgc
     5221 cgccggttga ggaggtcaag gccggcatcg cgatcctgga gacgctgaac ctgcgcgaac
     5281 gcggcgtgga cctggtcgcc tgcccgtcgt gcggccgcgc cgaggttgat gtgtacgcgc
     5341 tggcgggcgc ggtcgagaag cgcatgagcg agatcgacgt gccgatgcgc atcgcggtca
     5401 tgggctgcgt cgtcaacggc cccggcgagg cgcgcgaagc cgacgtcggc gtcgccgccg
     5461 gcaaaggcaa ggggcagatc ttcgtccgcg gcgaggtgct gcgcaccgtg cccgaggacg
     5521 acatcgtcga cgagctgttc aaagaggccc accgtctggc cgacgagctg cgcgccgagc
     5581 gcggcgacgg cacgcctgcc gtcgtcagtg tcgccccgca gcttcagtcc ctcatgcaga
     5641 tcggcaaggg cccgtcgaag ctcgagcgca cccaaggctg acgtacgtcc gtcgaaccca
     5701 tctgctgcaa acacctctcg cgacacagac ggcacgacgc tgcctgtcgc cgtctgtgtc
     5761 gcgagaggtg tttgcagcag atgggttcga cggacgtacg tcagccttgg gtgcgctcga
     5821 gcttc
//
LOCUS       DATLFO010000039         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_129814, whole genome shotgun sequence.
ACCESSION   DATLFO010000039 DATLFO010000000
VERSION     DATLFO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129814"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2052)
                     /locus_tag="VM841_01355"
     CDS             complement(<1..>2052)
                     /locus_tag="VM841_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HVL88858.1"
                     /translation="DFETSNWAWDPVANAYYWHRFYSHQPDLNFDNPAVRKAMMEVLD
                     FWFDMGVDGLRLDAVPYLFEREGTNGENLKETHEFLKELRGHVEERYGGEKMLLAEAN
                     QWPEDAAAYFGDGDECHMAFHFPLMPRMFMATRLEDRFPIIDILEQTPAVPDNAQWAL
                     FLRNHDELTLEMVTDEERDYMYRVYAADPQARINLGIRRRLAPLLGNNRRMIEMMNGL
                     LFSLPGTPVMYYGDEIGMGDNIYLGDRNGVRTPMQWSADRNAGFSRANPQKLFLPVII
                     DPEYHAEALNVEAQRANPHSLLWWTKRLIALRKQSKALSRGTLEFLYPENPKVLAYLR
                     EHDGERVLVVANLSRHAQYVELGLRDYAGMVPVEMFGGTEFPPIGELPYLVTLGPHAF
                     YWLALEDRDGEAAAGHGDGYPVLEVSGSWTDVLAKRGGRGPLERTLPGWLRTRRWFGA
                     KGRKIKAVDIDDTVPLSRTRGAASGVMAVLTVEYTEGEPETYALPLVWRHFEDGHEPP
                     TVIARVKCRDGEGLLFDGLSDPELCKALLSAVASRRTLRGAHGELTGTPTRAFRATRG
                     SSADELEVEWIGAEQSNTSVVFGDRIVMKLIRHLTDGVNPDLEVGRYLSEQTPFDRLP
                     RVSGAIEYRRGRAEPSTVAILTEYVDNEGDAWSFTLGQLGLFVERALVAQDDHPLTLP
                     HG"
BASE COUNT          318 a          711 c          682 g          341 t
ORIGIN      
        1 gccgtgcggc agcgtcagcg ggtgatcgtc ctgcgccacc aacgcccgct cgacgaatag
       61 cccgagctgg ccgagcgtga acgaccacgc gtcgccttcg ttgtcgacgt actcggtcag
      121 gatcgcgacc gtggacggct cggcgcggcc gcggcggtac tcgatcgcac ccgacacgcg
      181 cggcagccgg tcgaacggcg tctgctcgct caagtagcgg ccgacctcca ggtcgggatt
      241 cacgccatcg gtcaggtgcc ggatgagctt catcacgatc cggtcgccga acaccaccga
      301 ggtgttggat tgctccgcgc cgatccattc gacctcgagc tcgtcggcgc tcgagccgcg
      361 ggtggcgcgg aacgcgcgtg tcggcgtccc ggtcagctcg ccgtgcgcgc cgcgcagcgt
      421 ccggcggctc gcgacggcgc tcagcagcgc cttgcacagc tccggatcgc tcagcccgtc
      481 gaagagcagg ccctcgccgt cgcggcactt gacgcgcgcg ataacggtcg gcggctcgtg
      541 gccgtcctcg aagtgccgcc acaccagcgg cagcgcgtag gtctcgggct cgccttcggt
      601 gtattcgacc gtcaggaccg ccatgactcc cgaagcggcc ccgcgcgtgc gtgacagcgg
      661 cacggtgtcg tcgatgtcca ccgccttgat cttgcgcccc ttcgcgccga accagcggcg
      721 cgtgcgcagc cagccgggca gcgtgcgctc gagcggaccg cggccgccgc gcttggcgag
      781 cacgtccgtc cacgagccgc tcacctcgag caccgggtag ccgtcgccgt gtccggcggc
      841 ggcctcgccg tcgcggtcct ccagcgcgag ccagtagaac gcgtgcgggc cgagcgtgac
      901 caggtacggc aactcgccga tcggtgggaa ctccgtgccg ccgaacatct cgaccggcac
      961 catgccggcg tagtcgcgca gacccagctc gacgtactgc gcgtggcgcg acaggttcgc
     1021 caccaccagc acccgttcgc cgtcgtgctc gcgaagataa gcgagcacct tcgggttctc
     1081 cggatacaga aactccagcg ttccgcgcga caacgccttc gactgcttgc gcagcgcgat
     1141 cagccgttta gtccaccaca gcagcgaatg cgggttcgcg cgctgggcct cgacgttgag
     1201 cgcctcggcg tggtactcgg gatcgatgat gaccggcagg aacagcttct gcggattcgc
     1261 gcgggaaaag cccgcgttgc ggtcggccga ccattgcatc ggcgtgcgca cgccgttgcg
     1321 gtcacccagg tagatgttgt cgcccatgcc gatctcgtcg ccgtagtaca tgaccggcgt
     1381 gccgggcagg ctgaacagca gcccgttcat catctcgatc atgcgacggt tgttgcccag
     1441 cagcggcgcg agccggcggc gaatgcccag gttgatgcgc gcctgcgggt cggcggcgta
     1501 gacgcggtac atgtagtcgc gctcttcgtc ggtgaccatc tcgagcgtga gctcgtcgtg
     1561 gttgcgaagg aacagcgccc actgcgcgtt atcggggacc gcgggcgtct gctccaagat
     1621 gtcaatgatc gggaagcggt cttccaaacg cgtggccatg aacatgcgcg gcatcagcgg
     1681 aaagtggaag gccatgtgac actcgtcacc gtcgccgaag taggcggccg cgtcctcggg
     1741 ccactggttc gcttcggcga gcagcatctt ctcgccgccg tagcgctcct cgacgtggcc
     1801 gcgaagctcc ttgaggaact cgtgagtctc tttcaggttc tcgccgttgg tgccttcccg
     1861 ctcgaacagg tagggcaccg cgtccaggcg caggccgtcc acgcccatgt cgaaccagaa
     1921 gtccagcacc tccatcatcg ccttgcggac cgccgggttg tcgaagttca agtccggctg
     1981 atgcgagtag aagcgatgcc agtagtaggc gttcgcgacc ggatcccacg cccagttcga
     2041 ggtctcgaag tc
//
LOCUS       DATLFO010000040         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_80081, whole genome shotgun sequence.
ACCESSION   DATLFO010000040 DATLFO010000000
VERSION     DATLFO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80081"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VM841_01360"
     CDS             complement(<1..494)
                     /locus_tag="VM841_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL88859.1"
                     /translation="MTAAEAAELLERARFEVKRWIVGQDAMLERVVAALLAGGHVLLE
                     GVPGLAKTMTIRTVAAVFGGEFRRIQFTPDLVPSDLVGTRVLDSKSGSFRVELGPVFT
                     NFLLADEINRAPAKVQSALLEVMQERQVTIGRTTHTVPDPFLVMATQNPIESEGTYPL
                     PEAQV"
     gene            complement(624..1067)
                     /locus_tag="VM841_01365"
     CDS             complement(624..1067)
                     /locus_tag="VM841_01365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL88860.1"
                     /translation="MSLFRVIAHIDASPARVWDVLRDWEGSAEWMVDATTVEVIGEQR
                     EGAGTRVRAVTKIAGMPLTDEMVVTDWVPERLLQVRHEKFPIIGPAWFALAPAGRGTR
                     FEWGEDLRPPLGKLGELGGAILRTPIERVLAESLHKLKRVCESGG"
     gene            complement(1064..2314)
                     /locus_tag="VM841_01370"
     CDS             complement(1064..2314)
                     /locus_tag="VM841_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL88861.1"
                     /translation="MTIASGFRFKAVMAVTFFIMFGYGMIVPTLPLFASTLGVAETGI
                     GLVFTAFAGARLAASIVAGSAIGRFGERRMAAIGAFIVGLSSAAAGFSPNFSSLVLLR
                     GFGGIGSAFFLAALMTSLLGAVKPEERSRAMALFWAVVGVGFIVGPVFGGLAAVISLR
                     APLFFYGGICMAAAPLCYMVLGPGPRGATAIGGNSPVAEEPAPIPAVPGWQALKRLLR
                     DRAYRAALAVGAASFFVMGGVDPLVAKTWTDGFGMSASSVGIPFMLAGLATLGPAWHA
                     GKLGDLKGRKYATVPALLVSAIALSMLGLFDSPVAFAMMFTVVGLSWGYQRSGPSAIV
                     GDVSTDTDRAMAVGGFRIATDVGAFLGPALLGAVGEHASHGTAFVAAGVVVLLAFFAM
                     LPVRETAPAIAGAAAREPQAADSA"
BASE COUNT          425 a          847 c          824 g          295 t
ORIGIN      
        1 acctgcgcct cgggcagcgg gtaggtgccc tcggactcga tcgggttctg ggtcgccatc
       61 acgaggaacg ggtcgggcac cgtgtgcgtc gtgcggccga tcgtcacctg gcgctcctgc
      121 atgacctcga gcagcgcgga ctgcaccttg gcgggcgcgc ggttgatctc gtcggcgagc
      181 aggaagttgg tgaacaccgg tccgagctcg acacggaacg agccggattt cgaatccagc
      241 acgcgcgttc cgaccagatc cgaaggaacc aggtcgggcg tgaactggat gcggcggaac
      301 tcgccgccga agaccgcggc gacggtccgg atggtcatcg tcttggccag gccgggaacg
      361 ccttcgagca gcacgtgccc gccggcgagc agcgcggcca cgacgcgttc gagcatcgcg
      421 tcctgtccca cgatccaacg cttgacttcg aaacgggcgc gctcgagaag ctcggcggct
      481 tcggcggcgg tcatcgcggg ctctgcgtgt ttgctcacgg atgcgctcct tgatcccggg
      541 gtcgagcggg ggctcgcgcg aatgtcacca cgacagcgtg aacgcccgct gagaggaacg
      601 caagagcagg ctgtgagcag gcgctatccg ccggattcgc agacgcgctt gagcttgtga
      661 aggctttcgg cgagcacccg ctcgatcggc gtgcgcagga tcgcgccgcc gagctcgccc
      721 agcttgccga gcggcggacg caggtcctca ccccattcga accgggttcc gcgtcccgcg
      781 ggcgccagcg cgaaccaggc aggcccgatg atcgggaact tttcgtgacg gacctggagc
      841 agccgctcgg gaacccagtc ggtcacgacc atctcgtcgg tgagcggcat ccccgcgatc
      901 ttggtcaccg cgcgaaccct cgttcccgcg ccttcacgct gctcgccgat cacctcgacg
      961 gtggtcgcgt ccaccatcca ctcggccgag ccctcccagt cgcgaagcac gtcccacacg
     1021 cgggcgggcg acgcgtcgat gtgcgcgatg acgcggaaca ggctcatgcc gaatccgcgg
     1081 cctgcggctc gcgcgccgct gcgccggcga tcgcaggagc ggtctcgcgc accggcagca
     1141 tggcgaagaa tgcgagcagc accacgacgc cggcggccac gaacgcggtg ccgtggctcg
     1201 cgtgctcacc gaccgccccg agcagcgccg gcccgaggaa ggcgccgacg tcggtggcga
     1261 tgcgaaagcc gccaaccgcc atcgcgcgat cggtgtcggt ggacacgtct ccgacgatcg
     1321 cactcggtcc ggaacgctga tagccccacg acaacccgac gacggtgaac atcatggcga
     1381 aggcgaccgg cgagtcgaac agcccgagca tcgacagcgc gatcgccgac accagcagcg
     1441 ccggaaccgt cgcgtacttg cggcccttca ggtcgccgag ctttccggca tgccaggccg
     1501 ggccgagcgt ggcgaggccg gcgagcatga acgggatgcc gaccgaggac gcggacatgc
     1561 cgaagccgtc ggtccacgtc ttggccacga gcggatcgac gccgcccatc acgaagaacg
     1621 atgcggcgcc cacggcgagc gcggcgcgat aggcgcggtc gcgaagcagc cgcttgagcg
     1681 cctgccagcc ggggaccgcc gggatgggcg cgggctcttc ggcgaccggc gagttcccgc
     1741 cgatcgcggt cgcgccgcgc ggccccgggc cgagcaccat gtagcacagc ggcgcggccg
     1801 ccatgcagat gccgccgtag aagaacagcg gagcgcgcag gctgatcacg gcagccagcc
     1861 cgccgaagac cggcccgacg atgaagccga cgcccacaac cgcccagaaa agcgccatcg
     1921 cgcgagagcg ctcctcgggc ttgaccgcgc ccagcaggct cgtcatcagc gcggcgagga
     1981 agaacgccga tccgatgccg ccgaagccgc gcagcagcac gagcgaggag aagttcggcg
     2041 agaagccggc cgcggccgat gacagcccca cgatgaacgc accgatcgcg gccatacgcc
     2101 gctccccgaa tcgtccgatc gccgatcccg ccacgatcga ggcggccaga cgcgcgcccg
     2161 cgaaggcagt gaacaccagc ccgatccccg tctcggcgac accgagcgtc gaggcgaaca
     2221 gcggcagcgt cggcacgatc atgccgtagc cgaacatgat gaaaaaggtg acggccatga
     2281 ccgccttgaa gcggaagccg gatgcgatcg tcatggagcg gccatgatag tccgggccgc
     2341 caggcccggc tttcggatcc ccgcgactag gcttcgacga cgccgccgaa c
//
LOCUS       DATLFO010000041         5897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_40789, whole genome shotgun sequence.
ACCESSION   DATLFO010000041 DATLFO010000000
VERSION     DATLFO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40789"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..2578
                     /locus_tag="VM841_01375"
     CDS             386..2578
                     /locus_tag="VM841_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88862.1"
                     /translation="MRPFRRIIPLALIGISILTGPAARADNDAPRVVSLSRVTTAPAA
                     GQPVRLDVEVSDGGTSTVTHVQAVYRGPRGHFFVPSVHFGQSSGRGVASATLNAWAAT
                     GGWSLTEVIVYDRADNRTHYRRDGSTQTTPSGARPAEPTSIDFAAADFELTNANEDVT
                     APSIDSIRAVFGTVIAGTAQAAIYEASDDRSGIASVSVQYFLPGRAVMFLSSPPADLI
                     GVGPVAGVVPLFTEGGHATLAHVAVIDRADNRTIFSVNNGVLYSPGGIEPAATVNVDR
                     AKVEFDVQAVAPDGDYPSVESFEMRSSRVLRAGERVTIAFSVSDPTTLVSQVGVGWRD
                     QYGHQINEWYQCVGLREGTISATIPSFVEPGTRWALHQISVMDRAKHGTGYRSDGVTT
                     REAGNVVTPHATHTFDLSAGDFEIEAGAPRQENLPAPKVCDRPTLLDVVADPSEVASG
                     ETSSLRGSVRAGGAPVGDPVAALYEYEAGRPKLLNVVRGASGGTFARTIAPENGKTYR
                     ARFLGSDGHNPADPAFSPPVQVRVVGAGKHRSALHGSAPDWIRAGAPATVAALLTDAD
                     TSVALAGRTVVLEARPAGAAAFRRVATAATGSDGRVRWTVRPDSTTDYRVSFAGDAGA
                     EPVVSAPRRVQVAPRTWLLADRTRLPRGATASLTTGVAPSHAGHTVVLLRRAQDRWVR
                     VATARLDGSSRAVFRFQPPAGSWTYSVATSSHADHRWSQSPSVTLSVG"
     gene            2971..>5897
                     /locus_tag="VM841_01380"
     CDS             2971..>5897
                     /locus_tag="VM841_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL88863.1"
                     /translation="MRLLGPFEIRLPSGRAAGPWARPTARRLVQLLLLSRDHRIGREE
                     VIELLFPGLGPERAANAVSKALSMARRSMADAFTEIILADRSTIWIDVNVDLRIDLEL
                     HEQELHGAIGMPPGIERDDALEQALLRGGRLLDEELYADWAIPRRDHVDQLRRDARLA
                     LARDRSAGHGRADPVAVLDAWSGVFSSDPTCEEAAISLMQAFARQGRRDEAVRTYHRC
                     RAALRRLLDTEPSRALEVALDEAQRAPILVRPPTSLEVVSFGREGVLDEMLSHLDRTP
                     VGGGPSLAVLGPAGIGKTHVLLVVRSRLIGQGWRVLHATSIPADAVVPYAAIRTALAP
                     LADRVREGSLLARVMGKGSQRERAGSVEASEQQMVLAGEIADLLDDASSDGPLALVLD
                     DSQWMEPALAAVTARLVSRPAPRGWAILIAARTDEPHAPVPPLASGVIRIDLEPMPAE
                     PLAQVIRDRLGLRVELWSDELRQVVARSGGNPLFAVELGRAHLLTPGAAEASVPETIS
                     GLFKSHLAAATREGRSLVPVVALAGDAAPFELVLHVGAQLLGSEPAAIEAIDDLLDQG
                     LLTEDPAGVRLIHPLLRDTALASLNPVRRAALHEALATSIEQIGGDEAGVRSAHHRIA
                     AFMAARLRRHAEAAVREGSAAADAAFEVYAEGPALELYRGVLAAFNALPAADRAPWRA
                     HAVAAWLRVASILTRRYEVDEAQRALKEALAMAETDQERSSAWSGLASIPYRRGDFAA
                     VISLLETGLASLRDGSDLISAQMQVAIAWSKLRDGRTGEATDGLQAALPVLEEAGAVR
                     AVFLCLDYLALALEAAGAHDEAADAWSRAESTLNLAAEMEGEASSRWARHSRAMFVLH
                     RALRPLLMGDATTAVRDLEQALQAYLPLGDRYEMSVAHGLLASAYQMNGDLAAALERW
                     ETAATCLEESGHWYHLAGIHASRAGVLEALRRTDEAAAAADLARRIARDDASPQTIDE
                     IEE"
BASE COUNT          881 a         2018 c         2046 g          952 t
ORIGIN      
        1 cccgccagtt attgcaagct cgcgcttctg agcgttgctt gaccagcgcg aacaacggta
       61 acgaacagtt ccaacgtcct actttcacct ccaccaagag cttcttgttc gaacggtgaa
      121 agttcggccc gaaccaagac ccgtcgaggg attggccggt cgacgacgct cttgttcttg
      181 tgtcaatacg gcgttgctcg gcacgcccga ttgacacaag gtcgaccggc tgacttcgcc
      241 gatcgccacg cgtggagcca tccagctcgg gatcgagtct cggccgcaaa agcgatcgcc
      301 aatgtccgat tcatgcccgg gccggtgcgg tcttcgtcac cgcggcccga cggtctcgtt
      361 atcgatctga cgacctacac cttcaatgcg accatttcgg cgaatcatcc ccctcgcgct
      421 gatcgggatc tcgatcctga ccggccctgc cgcgcgtgcc gacaacgacg ctccgcgtgt
      481 cgtctcgctg tcgcgcgtca ccaccgcgcc cgccgccggg cagccggtgc ggctggacgt
      541 cgaggtctcc gacggcggca cgtccaccgt cacgcacgtg caggccgtct accgcgggcc
      601 ccgcggtcac ttcttcgttc ccagcgtcca cttcgggcag tcgagcggac ggggtgtcgc
      661 ctcggcgacc ttgaacgcgt gggcggcgac gggaggatgg tcgttgaccg aggtcatcgt
      721 ctacgaccgc gccgacaacc gaacccatta tcgccgggac ggctcgactc agaccacgcc
      781 gtcgggcgcg cgcccggccg agcccacgag catcgacttc gccgccgccg acttcgagct
      841 gacgaacgcg aacgaggacg tgaccgcgcc ttcgatcgat tcgatccgtg ccgtgttcgg
      901 caccgtcata gccgggacgg cgcaagcggc gatctacgag gcgtcggatg accggtccgg
      961 gatcgcgtcg gtgagcgtcc aatacttcct gcccggcaga gccgtcatgt tcctgtccag
     1021 cccgccggcc gacttgatcg gcgtcggccc ggtcgccggc gtcgtcccgc tgttcaccga
     1081 aggcggacac gcgaccctcg ctcatgtggc cgtcatcgac cgcgcggaca accggacgat
     1141 cttctcggtg aacaacggcg tcctgtactc gcccggcggc atcgagccgg ccgcgaccgt
     1201 caacgtcgat cgggcgaagg tcgagttcga cgtgcaggcc gtcgctcccg acggcgacta
     1261 cccgtcggtc gaatcgttcg agatgcgctc atcgcgtgtg ctgcgcgccg gcgagcgtgt
     1321 cacgatcgcc ttctcggtct cggatccgac gacgttggtc tcgcaggtcg gcgtcggatg
     1381 gagagatcag tacgggcatc agatcaacga gtggtaccag tgcgtcgggc ttcgcgaagg
     1441 gacgatctcg gcgaccattc cgagcttcgt cgagccggga acgcgatggg ctctccacca
     1501 gatctcggtg atggatcgag cgaagcacgg gaccggctac cgcagcgacg gcgtcaccac
     1561 tcgtgaggcc ggcaacgtgg taacgcccca cgccacgcac acgttcgacc tgtccgccgg
     1621 cgatttcgag atcgaggccg gcgcgccgcg gcaagagaac cttccggcgc cgaaggtgtg
     1681 cgaccggcct acgctcctgg atgtcgtcgc cgacccgtcc gaggttgctt ccggggagac
     1741 gtccagcctt cgcggaagcg tccgagccgg tggcgcgccc gtcggcgacc cggtggcggc
     1801 gctctacgag tacgaggccg ggcgaccgaa gctgctcaac gtcgtccgcg gcgcgtcggg
     1861 cggaacgttc gctcgcacga tcgcgccgga gaacggcaag acgtatcgtg ctcgcttcct
     1921 cggctccgac ggccacaacc cggcggatcc cgcgttctcg ccgccggttc aggtgcgtgt
     1981 cgtcggcgcc ggcaagcacc gcagcgcgct gcacggctcg gcgccggatt ggatccgcgc
     2041 cggagctccg gcgacggtcg ccgctctgct gaccgacgcg gacacctcgg tcgcgctggc
     2101 cgggcggacc gtcgtcttgg aagcgcgtcc cgccggcgcg gccgcgttcc ggcgcgtcgc
     2161 gaccgcggcc accggcagcg acgggcgcgt ccggtggacg gtccggccgg actccaccac
     2221 cgactatcgc gtgagcttcg ccggcgacgc gggagccgag cccgtcgtct ccgcgccgcg
     2281 gcgtgtgcag gtcgctccgc ggacctggct gctcgccgac cgcacacggc ttccgcgcgg
     2341 agcgaccgcc tcgctcacga ccggcgtggc gccgtcgcac gcggggcaca cggtcgtcct
     2401 gctgcgacgt gctcaggatc gctgggtgcg agtcgcgacg gcgcggctcg acggttccag
     2461 ccgagccgtc ttccggttcc agccgccggc gggatcctgg acgtactcgg ttgccacgtc
     2521 ctcccacgcc gatcaccgct ggtcgcagtc gccctccgtg acactgtccg taggctaagg
     2581 cccgatcgta gagaacacgc tcgcccgccc tcgccagctg caccgcgcgt cgccggagct
     2641 cctgcggatg tggtcgtggc atccagactc ccctgcgttt ccgcaggtca gggcggttcg
     2701 aatagcgacc gttcacctcc acagcccagt tctccaggtg ttacgtgccc aacagagttc
     2761 gaagctcgaa cgccccgact tgagctaacc ggagctctcg ctgatcgaac cgttcacttt
     2821 ctcgccgccg cgtgggcgtc gtacagttct gtcgggatga tcgacggcag ggagtgtgat
     2881 gcgagggggc tcgtgactgc agcggctact gggggggctc cggcggatac agcgacgcaa
     2941 tcatccggcg agccacgccc gatcgttcag gtgcgtctgc tcggaccatt cgagatccgc
     3001 ctgccgtccg ggcgcgcggc cggtccctgg gcccgcccga cggcacgccg tctggtccaa
     3061 ctgctcttgc tgagtcgcga ccaccgcatc gggcgcgagg aggtcatcga gctgctgttt
     3121 ccggggttgg gtcccgagcg cgcggcgaat gccgtttcga aggcactctc gatggcgcgc
     3181 cgctccatgg ccgacgcctt cacagagatc atcctcgccg accgatccac gatatggatc
     3241 gacgtcaacg tggatctgcg gatcgacctg gagctccatg aacaagagtt acatggcgcg
     3301 atcggtatgc cgcctgggat cgaacgcgac gacgcgctgg agcaggccct gttgcgtggc
     3361 gggcgcctgc tcgacgagga actgtatgcg gactgggcga taccaagacg ggatcatgtc
     3421 gaccagctcc gacgtgacgc gcggctagcg ctggcgcggg atcgctccgc cggacacgga
     3481 cgagccgatc cagtcgccgt cctggacgcg tggagcggtg tcttctcgtc ggacccgaca
     3541 tgtgaggaag cggcgatctc cctgatgcag gcattcgcgc ggcagggtcg ccgagacgag
     3601 gcggtgcgga cgtatcaccg ttgccgcgcg gcgcttcgcc gtctattgga cacggagccg
     3661 tcgcgggccc tcgaggtggc actcgatgaa gcgcaacggg cacccatcct cgtgcggccg
     3721 cccacctccc tcgaggtggt ttcgtttggg cgggaaggag tgctcgatga gatgctctct
     3781 catctcgatc gaacgcccgt tggcggcggg ccgtccctgg ccgtcctcgg cccggcgggg
     3841 atagggaaga cccacgtgct cttggttgtc cgttcgcggc tgatcgggca agggtggcgg
     3901 gttcttcacg ctacgtcgat ccccgccgac gcggtcgttc cctacgcggc gatccgtacc
     3961 gcgcttgccc cgctggcgga cagggtccgc gaagggagcc tgctcgcgcg cgtcatgggg
     4021 aagggatctc agcgcgagcg ggcagggagc gtggaagcct cggagcagca gatggtgctg
     4081 gccggcgaga tcgccgacct cctcgacgac gcgagcagcg acggcccgtt ggccttggtg
     4141 ctcgacgact cgcagtggat ggaaccggcg ctggcagcgg tgacggctcg gctggtgagt
     4201 cggcccgcgc ctcgaggatg ggcgatcctg atcgcagcgc gcacggacga gccccatgcg
     4261 cccgtgccgc ccctcgcgtc gggcgtcatc cggatcgatc tcgagccgat gccggctgag
     4321 ccgctggcgc aggtcatccg cgaccggctc ggcttgcggg tcgagctctg gagcgatgag
     4381 ctgaggcagg tcgtggcccg cagcggaggt aaccccttgt tcgcggtaga actcggccgc
     4441 gcgcacctcc ttacgccggg cgctgccgag gcgagcgtcc cagagacgat cagtggtctg
     4501 ttcaagagcc acctggcggc cgcgacccgc gaaggccgat ccctcgttcc tgtcgtggct
     4561 ctcgcgggtg acgcagcgcc attcgagctc gtcctccacg tcggggccca actgctcgga
     4621 tccgagccgg cggccataga agcgatcgac gacctgctcg accaaggcct gctgacggag
     4681 gatccggcgg gggtgcgcct gatccatccg ctcctgcgcg acaccgcgct cgcctcactg
     4741 aatcccgttc ggagggcggc cctgcacgaa gcgttggcaa cctcgatcga gcagatcggt
     4801 ggtgacgagg caggcgtccg ctccgcgcat catcggatcg ctgcgttcat ggcggcgcgc
     4861 ttgcggagac acgcggaggc tgccgtacgg gaggggtcgg cggccgcgga cgccgccttc
     4921 gaggtgtatg ccgaaggacc tgcgctggag ctgtaccgcg gagtcctcgc cgcgttcaac
     4981 gcgctgccgg cagcggatcg cgcgccctgg cgcgcgcacg ctgtcgcggc atggctccgc
     5041 gtggcttcga tccttacgcg gcgctacgag gtcgatgagg cgcagcgcgc gctgaaggag
     5101 gcgctggcga tggcggagac cgaccaggag cggagttcgg cgtggagcgg tctggcgagt
     5161 atcccgtacc ggagggggga cttcgccgcg gttatcagcc tgctcgagac gggcctggca
     5221 tccctcaggg atggctcgga cctgatcagc gcccagatgc aggtcgcgat cgcgtggtcg
     5281 aagctgcggg acggccggac cggtgaggca accgacgggc tgcaagccgc cctgcctgtg
     5341 ctcgaggagg ccggagcggt gagagcggtg ttcctatgcc tcgattacct ggcgctcgcg
     5401 ctcgaggcgg cgggtgctca tgacgaggcg gccgatgcct ggagcagagc agagtcgacg
     5461 ttgaacctgg ccgcggagat ggaaggcgag gcatcgagcc ggtgggcgcg gcactcgcgg
     5521 gccatgttcg tcctgcaccg ggcgctgaga cctctgctga tgggagacgc gacaactgcg
     5581 gtgcgcgacc tcgagcaagc cttgcaggcg tacctcccgc tgggcgaccg atacgagatg
     5641 tcggtggcgc acggcctcct cgcgtcggcg taccagatga acggcgacct cgcggctgcc
     5701 ctcgagcgat gggaaacggc cgcgacctgc ctcgaagagt cgggtcactg gtaccacctc
     5761 gcggggatcc atgcatcgcg agcaggggtc ctcgaagccc ttcgccgcac cgacgaggcc
     5821 gcggccgcag ccgatctcgc ccgtcggata gcccgcgacg acgccagtcc gcagacgatc
     5881 gacgagatcg aagagat
//
LOCUS       DATLFO010000042         6374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_110408, whole genome shotgun sequence.
ACCESSION   DATLFO010000042 DATLFO010000000
VERSION     DATLFO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110408"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VM841_01385"
     CDS             <1..390
                     /locus_tag="VM841_01385"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HVL88864.1"
                     /translation="GRVRSHPGDYESYLWKREKEEAEAAATAERAAAASQPKRSASSD
                     KERRRIEAQQRSGQRKVRDRIATIEREIETRTAEMKELEARLADPAFYATGDGVPEAI
                     QAYDAAKKRVEELEAEWDRATEEASRS"
     gene            complement(487..1068)
                     /gene="rdgB"
                     /locus_tag="VM841_01390"
     CDS             complement(487..1068)
                     /gene="rdgB"
                     /locus_tag="VM841_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017610385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HVL88865.1"
                     /translation="MRVGLATGNPGKVREIVAILGGDIEVVPPPPDWVAPEETGGTYI
                     ENARLKAQSLCDHTGAAAIADDSGIEVNLLKGAPGVRSARFAGDSATDEQNLNLLIDT
                     IRDVAEDERGARYRCLAVLILPDGTEHAAEGTVDGTLITEPRGTNGFGYDPIFVPRGY
                     DVTMAELDPAEKDSISHRGTAFRLLGGIIRGLS"
     gene            complement(1074..1796)
                     /gene="rph"
                     /locus_tag="VM841_01395"
     CDS             complement(1074..1796)
                     /gene="rph"
                     /locus_tag="VM841_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HVL88866.1"
                     /translation="MAGEAVTRAPDELRPVEITRGFQETAEGSALIRIGSTRVLCAAS
                     FEETVPRFLRGTGGGWVTAEYSMLPRATVVRTPREVKSGRPSGRTQEIQRLIGRSLRA
                     VTDLSALGENTITIDADVLLADGGTRCASITGCFVALYDACRAMLEAGKIERMPIREE
                     VAAISVGIVDGVPRIDLDYELDVAASVDMNVVMTGSGRFVEVQGTAEGDPFEKSELDR
                     LLELAEAGIKELIQAQRDALGI"
     gene            complement(1778..2533)
                     /locus_tag="VM841_01400"
     CDS             complement(1778..2533)
                     /locus_tag="VM841_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL88867.1"
                     /translation="MRLTVLGSCGTFPNAGGACSGYLLQHDDFTLWLDAGTGTLANLQ
                     KHVSIDDVDAIALSHMHADHFTDLYPFLYAVEFGPAGPRRVPVYAPPSGPDVFGRLLR
                     DESSAAFPKVYEWTGLEPGEEADIGPFRLRTFPAAHSIPNLTMRIEAGGEVFCYSGDT
                     GPNESLAHAAQNADLFLCEASWQDGENKIDVPIHLTAREAGRYAAEAGAKRLLLTHIW
                     PHYDLERSVDQAAEEYDGIVEIAHGGNAWQVKP"
     gene            complement(2530..3339)
                     /gene="murI"
                     /locus_tag="VM841_01405"
     CDS             complement(2530..3339)
                     /gene="murI"
                     /locus_tag="VM841_01405"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HVL88868.1"
                     /translation="MDSRPIGVFDSGVGGLTIVRGLIELMPDEEILYFGDTARYPYGP
                     RSRDEVRRFAMEIATWLAEQDVKMLVAACNSAASAGLDHVRDAFPDLHVVEVVEPAVR
                     AAIRATRNRRIGVIGTELTIASGAYQRALDATKENVMLFSQACPRFVEFVERGETAGP
                     EIIELAREYLAPLQQEDVDTLILGCTHYPLLQGVLHFVMRDAVLISSAEETAQDVYTE
                     LTQRNQFRVADTLPRHRFVSSGDAVTFQALGSRFLGPEIAVVEERSLGPVS"
     gene            complement(3365..4297)
                     /locus_tag="VM841_01410"
     CDS             complement(3365..4297)
                     /locus_tag="VM841_01410"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HVL88869.1"
                     /translation="MRYDDILASVGNTPLVGIHSLSPNPDVRFWAKLEGMNPTGSMKD
                     RIALRMITDAESSGALTRDKIVLEPTSGNTGIALAMICKRKGYRLTCVMPDNVSVERR
                     QILEMFGADMVFSPGELGSNGAIALAKELAADNKYFMLYQYGNDANWKAHYDGTGAEI
                     VRDLPEITAFVAGLGTGGTLMGCGRRIREHNPDVQTIAGEPEYGELVYGLRNLDEGFI
                     PPIVDLSVLSRRIKVGTRDALAATRELLDKEGIFSGISSGAAVHVAKRAAAKMEGGDV
                     VILLPDGGWKYLSTGAYTGSFEEAVERLEGTIWA"
     gene            complement(4304..4579)
                     /locus_tag="VM841_01415"
     CDS             complement(4304..4579)
                     /locus_tag="VM841_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HVL88870.1"
                     /translation="MAVEVRVPTILRKHTGNAKAVQASGSTVREVVESLDSEHPGLRE
                     AIVTEGGDIHRFINIYLNDEDIRFTGALDTPVSDGDVLSILPAVAGG"
     gene            complement(4634..5041)
                     /locus_tag="VM841_01420"
     CDS             complement(4634..5041)
                     /locus_tag="VM841_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HVL88871.1"
                     /translation="METFEIPEAMVADMVAHAQAEYPSECCGVIGGDGDGPRKLFRLR
                     NVDPDPVMRYAADPKELKRATDDIYDADWEIVCIYHSHTHSPAFPSPTDVERALYPEA
                     VYALVTLQDRQNPELRAFRIVEGTITELKVVRT"
     gene            complement(5120..6178)
                     /gene="selD"
                     /locus_tag="VM841_01425"
     CDS             complement(5120..6178)
                     /gene="selD"
                     /locus_tag="VM841_01425"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6134..6136),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HVL88872.1"
                     /translation="MESVRLTAYSHGAGUACKLGPAELAQVLRRLTPAAPAQRERLLV
                     GSETGDDAAVWKLDDATAIVFTADFFTPIVDDPFDWGRIAAANALSDVYAMGGTPILC
                     LNLVGWPRESLPLEVLSRVLDGGSAVAAEAGALIAGGHTIDDNEPKYGMAVVGLVSPD
                     RVVTNEGAKAGDVLVLTKPIGIGVIATAIKRGEAPPQVAAAAVEAMVTLNRGGAEAML
                     EAGVVAATDITGFGLLGHLHRMLESSGVAAVLDPEAVPLLDGARELAGRGFVPGGTER
                     NRAWVDRVVDWGAASEAVRTLLSDAQTSGGLLICCPPPAEDGLIAGLANRGIRGAVIG
                     TVEAGEPGRIALRPARGA"
BASE COUNT          976 a         2155 c         2225 g         1018 t
ORIGIN      
        1 ggccgggttc gctcgcatcc cggcgactac gagtcgtacc tgtggaagcg cgagaaggag
       61 gaggccgaag cggccgcgac ggccgagcga gccgcggcgg cgtcgcagcc gaagcggtcg
      121 gcctcgtcgg acaaggagcg ccgccggatc gaggcgcagc agcgcagcgg gcagcgaaag
      181 gttcgcgacc ggatcgccac gatcgagcgc gagatcgaga cacgcaccgc cgagatgaaa
      241 gagctcgagg cccgcctggc cgaccctgcc ttctacgcca ccggcgacgg cgtgcccgag
      301 gccatccagg cctacgacgc cgcgaagaag cgcgtcgaag agctcgaagc cgaatgggac
      361 cgcgcgactg aagaagcgag ccggtcctga aaggtcccgg gcggcgcgcg gtagcgactc
      421 cgcgccatgc cgcaccctgc cggggcccgg tcgcgcagaa ctgcaccaag caccggatga
      481 ccgggctcag gacagcccgc ggatgatacc cccgagaagc cggaaggcgg tcccgcggtg
      541 gctgatcgag tccttctcgg ccgggtccag ctcggccatc gtcacgtcgt aaccgcgcgg
      601 gacgaagatc gggtcgtagc cgaacccgtt cgttccgcgc ggttcggtga tcagcgtgcc
      661 gtcgacggtt ccttcggccg cgtgctcggt gccatcgggc aggatcagca ccgcgaggca
      721 gcggtagcgc gcaccgcgct cgtcctcggc cacgtcgcga atcgtgtcga tgagcaggtt
      781 caggttctgc tcgtcggtgg cgctgtcgcc ggcgaaacgt gcgctgcgga cgccgggcgc
      841 tcctttgagc aggttcacct cgatgcctga gtcgtcggcg atcgcggcgg cgccggtgtg
      901 gtcgcagagg gattgcgctt tcaggcgcgc gttttcgatg taggtgccgc cggtctcttc
      961 gggcgcgacc cagtccggcg ggggcggcac gacctcgatg tcgccgccga ggatcgcgac
     1021 gatctcacga accttgcccg gatttccggt cgccagcccg acgcgcatga cggtcagatg
     1081 cccagcgcgt cgcgctgcgc ctggatcagc tctttgatgc cggcttcggc aagctcgagc
     1141 agccggtcca gctcgctctt ttcgaacggg tcgccctctg cggtgccttg cacctcgacg
     1201 aagcggccgc tgccggtcat cacgacgttc atgtcgaccg aagccgcaac gtcgagctcg
     1261 tagtcgagat cgatgcgggg cacgccgtcc acgatgccga cgctgatcgc cgcgacctcc
     1321 tcgcggatcg gcatgcgctc gatcttgccc gcctcgagca tcgcgcggca ggcgtcgtag
     1381 agcgcgacga agcagccggt gatcgaggcg cagcgcgtgc cgccgtcggc gagcagcacg
     1441 tcggcgtcga tggtgatggt gttctcgccg agcgcggaca gatcggtgac ggcccgcagc
     1501 gaacggccga tcaggcgctg gatctcttgg gtgcgtccgc tcggccggcc ggacttgacc
     1561 tcgcgcggcg tccggaccac ggtcgcgcga ggaagcatgc tgtactccgc ggtcacccag
     1621 ccgccgcccg tcccgcgcag gaaccggggg acggtctcct cgaaggaggc cgcgcacagc
     1681 acacgcgtcg atccgatccg gatgagcgcc gatccttcgg cggtctcctg gaagccgcgc
     1741 gtgatctcga cgggccgcag ctcgtccgga gcacgggtca cggcttcacc tgccatgcgt
     1801 ttcctccatg agcgatctcg acgatcccat cgtattcctc ggccgcttga tcgaccgagc
     1861 gctccaggtc gtagtgcggc cagatgtggg tcagcaaaag acgcttcgcg cccgcctcgg
     1921 ccgcgtagcg tcctgcctcg cgtgcggtca ggtggatcgg cacgtcgatc ttgttctcgc
     1981 cgtcctgcca cgacgcttcg cacaggaaca ggtcggcgtt ctgggcggcg tgcgcgagcg
     2041 attcgttggg accggtgtcc ccgctgtagc agaacacctc tcccccggcc tcgatgcgca
     2101 tcgtcagatt ggggatcgag tgcgcggccg gaaaagtgcg aaggcggaag ggaccgatgt
     2161 cggcctcctc ccccggctcc agaccggtcc actcgtagac tttggggaag gccgcgctgg
     2221 attcatcgcg aagcagacgg ccgaacacgt cgggacccga cggcggcgcg tagaccggga
     2281 cgcgccgcgg cccggccggc ccgaactcga ccgcgtagag aaacgggtac aggtcggtga
     2341 agtggtccgc gtgcatgtgc gagagcgcga tcgcgtcgac gtcgtcgatg gagacgtgct
     2401 tttgcaggtt ggcgagcgtg cccgtgcccg cgtcgagcca gagggtgaag tcgtcgtgct
     2461 gcagcaggta gcccgagcag gcgccgcccg cgttcgggaa ggtgccgcag gagccgagca
     2521 cggtgagcct catgagacgg gcccgaggga acgttcctcg accacggcga tctccggccc
     2581 gaggaaccgc gaccccagtg cctggaaggt caccgcgtcg ccgctggaca cgaagcgatg
     2641 ccgcggcagc gtgtccgcga cccggaactg gttgcgttgc gtaagctctg tatagacgtc
     2701 ctgggcggtc tcctcggcgc tggagatcag gacggcgtcg cgcatcacga agtgcaggac
     2761 gccttgaagc agcgggtagt gcgtgcatcc caggatcaag gtgtccacgt cctcctgttg
     2821 aagcggcgcg agatactcgc gggcgagctc gatgatctcc ggcccggccg tctcgccgcg
     2881 ctcgacgaac tcgacgaacc gggggcacgc ctgcgagaag agcatgacgt tctctttcgt
     2941 agcgtccagg gcgcgctgat acgcgcccga ggcgatggtg agctcggtcc cgatcacacc
     3001 gatacgccgg ttgcgggttg ccctgatcgc agcgcgcacg gccggctcga ccacctcgac
     3061 cacgtgcagg tccgggaagg cgtcgcgcac gtggtccagg ccggcgctgg cggccgagtt
     3121 gcaggcggcg acgagcatct tgacgtcctg ctcggccagc caggtcgcga tctccatcgc
     3181 gaagcggcga acctcgtcgc gggagcgcgg accgtacggg tagcgggcgg tgtcgccgaa
     3241 gtagaggatc tcctcgtccg gcatgagctc gatcaggccc cggacgatcg tcagaccgcc
     3301 gacgcccgaa tcgaatacgc cgatcggccg tgaatccata cgggaccgga gtatagccgg
     3361 gcggctatgc ccagatggtg ccttcgagac gctcgacggc ctcttcgaac gaaccggtgt
     3421 aggcgccggt ggacaggtac ttccatccgc cgtcgggcag caggatgacc acgtcgccgc
     3481 cctccatctt ggcggccgca cgcttggcga cgtgcaccgc cgcacccgag gagatccccg
     3541 agaagatgcc ctccttgtcg agcagctcgc gcgtcgcggc cagcgcgtcg cgcgtgccga
     3601 ccttgatgcg gcgcgacagc accgacaggt ccacgatcgg cgggatgaat ccttcgtcga
     3661 gatttcgcaa gccgtagacg agctcgccgt attcaggctc gccggcgatg gtctgaacgt
     3721 cggggttgtg ctcgcggatg cgccggccgc atcccatcaa ggtcccgccg gtcccgagtc
     3781 ccgcgacgaa cgcggtgatc tcgggaaggt cgcgcacgat ctctgcgccg gtgccgtcgt
     3841 agtgcgcctt ccagttcgcg tcgttgccgt actggtagag catgaagtac ttgttgtcgg
     3901 ccgccagctc tttcgccagc gcgatcgcgc cgttggagcc cagctcgccg ggcgagaaga
     3961 ccatgtcggc gccgaacatc tcgaggatct gacgccgctc gaccgagacg ttgtcgggca
     4021 tgacgcaggt gagccggtag cccttgcgct tgcagatcat cgcgagcgcg attccggtgt
     4081 tgccgctggt cggttcgagc acgatcttgt cgcgcgtgag cgcgccggac gattcggcgt
     4141 cggtgatcat gcgcagcgcg atccggtcct tcatcgagcc ggtcgggttc atcccctcga
     4201 gcttggccca gaagcggacg tccggattcg gggagagcga gtggatgccg acgagcggcg
     4261 tgttgccgac cgacgcgagg atgtcgtcgt agcgcacggg cgctcagccg ccggcgacgg
     4321 ccggcagaat cgagagtacg tcgccgtcgg atacgggcgt gtccagcgcg ccggtgaaac
     4381 ggatgtcctc gtcgttgagg tagatgttga tgaagcggtg gatgtcgccg ccctcggtaa
     4441 cgatcgcctc gcgaagaccg gggtgctcgg agtcgagcga ctcgacgact tcgcgcacgg
     4501 tcgagccgct cgcctgcacc gccttggcgt tgcccgtgtg cttgcgcaag atcgtcggaa
     4561 ctctgacttc gactgccatg acctctgctt cctccccggt atcgctacag attacgccag
     4621 gtgaaattcg cccttacgtt cgaacgacct ttagttcggt gattgttccc tcaactatcc
     4681 gaaaagcccg aagctccggg ttctgcctgt cctgcagcgt gaccagcgca tagacggctt
     4741 cgggatacag cgcgcgttcg acgtcggtcg gtgacgggaa cgcgggcgaa tgcgtgtgcg
     4801 agtggtagat gcacacgatc tcccagtcgg cgtcgtagat gtcgtcggtc gcgcgcttga
     4861 gctccttggg atcggcggcg tagcgcatga ccggatctgg atcgacgttg cggagccgga
     4921 acagcttgcg cggcccgtcg ccgtcgccgc cgatcacccc gcagcattcg ctgggatact
     4981 cggcctgtgc gtgggcgacc atgtcggcca ccatcgcctc gggtatctcg aaagtctcca
     5041 tcgtcttttc gcctcggtca cggcggcccg gccgcccggc gggtgaggat atcagccccc
     5101 tcgcccggtt cgcgctcgct tacgcaccac gggccgggcg gagcgcgatt cggccgggct
     5161 cgccggcctc gaccgtgccg atgaccgcgc cgcgaatgcc tcgattcgcc agccccgcga
     5221 tcagaccgtc ctcggcgggg ggcgggcagc agatcagcaa gccgcccgac gtctgcgcgt
     5281 ccgacagcag cgtgcgcacg gcctcggacg cggctcccca gtcgacgacg cgatccaccc
     5341 aggcccggtt gcgctccgtc ccgcccggca cgaagccccg gccggccagt tcgcgcgcgc
     5401 cgtccagcag cggaacggct tcgggatcga gcacggcggc gacgccggag gactcgagca
     5461 tccgatgcag atggccgagc agcccgaagc cggtgatgtc ggtcgccgcg acgacgccgg
     5521 cctcaagcat cgcctcggcc ccgccgcgat tgagcgtgac catggcctcg acggcggctg
     5581 cggcgacctg cggcggggct tccccgcgct tgatcgccgt cgcgatcacg ccgatgccga
     5641 tgggcttggt caggaccagg acgtcgcccg ccttcgcccc ctcgttggtc acgacgcgat
     5701 cgggcgacac gagaccgacc acggccatgc cgtacttggg ctcgttgtcg tcgatcgtgt
     5761 ggccgccggc gatgagcgcg cccgcttccg ccgcgacggc cgatcctccg tcgagcacac
     5821 gcgagagcac ctcgagcggc agcgactcgc gcggccagcc cacaaggttc aggcacagga
     5881 tgggcgtgcc gcccatcgcg tacacgtccg agagcgcgtt cgcggcggcg atgcggcccc
     5941 agtcgaacgg atcgtccacg atgggcgtga agaagtcggc ggtgaacacg atcgcggtcg
     6001 catcgtcgag cttccacacg gcggcgtcgt cgccggtttc gctgccgacc aggagccgtt
     6061 cccgctgcgc gggagcggcg ggtgtcaggc ggcgcagaac ctgcgccagc tcggcggggc
     6121 cgagcttgca cgctcagccg gcgccgtggc tgtatgcggt cagccgtacg gactccatgt
     6181 cggtcacccc cttccggtcc cgaggcggtc attctcccac ggacgatagg ctggcgcgcg
     6241 gagcccgcca tggatgcgat caggatcgac ggagccgacg cgaggcggcg gctgctgatc
     6301 gccggcgcga tcgcgggcgc cgtgtggggc tttctcggct acttcgtcct gtggggctac
     6361 acctcgatca cggt
//
LOCUS       DATLFO010000043         7721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_91320, whole genome shotgun sequence.
ACCESSION   DATLFO010000043 DATLFO010000000
VERSION     DATLFO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91320"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VM841_01430"
     CDS             <1..768
                     /locus_tag="VM841_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL88873.1"
                     /translation="GIRNPDIVAPGRSVQSLRSAGSFIDQTYPGGIIGGRFFRGSGTS
                     QAAAVVSGAAALLLQQRPELSPDQLKHIMKSTATTLPAADPIAQGHGMLDLRSASRAM
                     TPLSIVAAQALPSATGTGSLDLARGSARVSMDGVILNGERDIFGRAWNGAAWAPAALA
                     ETSWNGGTWNGATWSGASWSGASWSGASWSGASWSGASWSGASWSGASWSGASWSGAS
                     WSGATWSGASWSGATWSGATWSGATWSGASWSSAGWS"
     gene            complement(826..1383)
                     /locus_tag="VM841_01435"
     CDS             complement(826..1383)
                     /locus_tag="VM841_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HVL88874.1"
                     /translation="MDTIKLLERAAAEFRSRLEKVTDWTVDTPCTEWDARALSNHVVN
                     EIMWIPPLVEGKTIAEVGPSLDGDLLGDDAVAAWDAATERVLSILSQPGALERPVQLS
                     YGERDTAGYVTEVTADITVHTWDLARAVGADERLDPVLVDLATGYLTPVIEGAREAGV
                     FAAAVLVPDDADPQTKLIALTGRTP"
     gene            1467..2291
                     /locus_tag="VM841_01440"
     CDS             1467..2291
                     /locus_tag="VM841_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HVL88875.1"
                     /translation="MSDLTLMAVHAHPDDEAIGTGGLLARCAAEGVRTVLITCTNGEL
                     GDLPGGVKPDHEEHDVAAVVAARREELRRSCDILGVSHLEMLGYHDSGMMGWPQNEHP
                     HAFWHTPVDEAAAKIAALMETYRPQVVVTYDENGFYGHPDHIQANRATLAAAAMCGVP
                     RKIYYTAVSRQSIGRLARLVRDTMPDRAGDWEFDPDDPPFGIDEAEITTVLDVSEFIR
                     RKRDAMAAHASQSENIEFLRMPDELFGQVFGAESFVRVRDDTGAPLPETDLFAGLR"
     gene            2396..2539
                     /locus_tag="VM841_01445"
     CDS             2396..2539
                     /locus_tag="VM841_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88876.1"
                     /translation="MRHPVRRPEVAMKITLGVSWNDIKACTAALLALVVPLALRRPAR
                     AGT"
     gene            2545..5238
                     /locus_tag="VM841_01450"
     CDS             2545..5238
                     /locus_tag="VM841_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL88877.1"
                     /translation="MNTTPATESPGRGPGAPGARFTGAQRVWILTGIIAVISLAMYVG
                     LVLGHEPIKPSYEIPWWVLAAGFFAAEIFVVHIQLRRDDYSFSLSEIPLVLGLFFASA
                     EAVIFAHVVGAGAALIVHRRQSLLKLAFNVSHFLLEVSLAVAAFRLFAGVQVTVGVMD
                     SIAAMLVTLIATWAALFMIFTAVAMSQGSRNLTGLSRALVLGSVTTLTNSSIGLVGVT
                     ILGSSPASIWLLAVPSITLFIAYRAYTSEQTKHESIEFLYQSTSILHKAQEMESAMLS
                     LLEQTRQMFRAEIAQIVLLSSDGQKPLRTTLGPGETAEIMRPDGQPAGVFRMVVDERR
                     GVRLSRPIRSDDFRARFAPTQVKDAMIAPLRGETHVLGQLIVVNRLGDVTSFDDEDLK
                     LFETLANHASIALENGRLEKSLAEVTRLKEELRHQAYHDPLTSLANRSLFLDRLRHAL
                     ARRRTAHREVAVIFVDLDDFKTVNDSLGHAAGDQLLCGVADRLRGICRPADTVARLGG
                     DEFAILLEESTPVETIAVAERLIDELETPFELAHKEIYVRASVGIASHGHAGDAESLM
                     RNADAAMYQAKSHGKGRFELYDPSMQKAALNRLEMKGRLQRAVERDEIEIFYQPTIAL
                     ATGNIHGAEALVRWRQPGSRRLTPVEFIPLAEETGLINVVGRHVLEQACLRMREWTER
                     YPVLGPLKIAVNISARQLQQPDLVEIVGEAVSAAGLSPSQLVLEITESVLLQDTDATI
                     RLLQELKALNVQLAIDDFGTGYSSLSYLQRLPMDIIKIDKSFVHGVGGSAEDEALTRA
                     IVKLAQTFRLKVVAEGIERQEQMDRLRQMRCHLGQGYLFSPPVPAQRFEELLGAAVGG
                     ADAELIDFATAVKSLPGRAPATSAARSDAMAKDALPYDATR"
     gene            complement(5220..5882)
                     /locus_tag="VM841_01455"
     CDS             complement(5220..5882)
                     /locus_tag="VM841_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVL88878.1"
                     /translation="MKKLAITAIAAVALPLTALPSGASPDPAPAPVPDHLAGGRLAAL
                     LSTEPAAVATGVDGPVRIGLSALRVIEPVKIAIRPADLARAREARERAKAVPRRVESR
                     AARSARSSPPTAASSGAASASGGGGTLASWYNDRPSACWDRRGRHAFPKGLTLWTAHK
                     TLPCGTAVAVTGPAGTMTVRVYDRGPYVAGRGLDLSAAAFRGVCGSTSRGVCRVSYRV
                     AS"
     gene            complement(6177..7262)
                     /locus_tag="VM841_01460"
     CDS             complement(6177..7262)
                     /locus_tag="VM841_01460"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HVL88879.1"
                     /translation="MRIGIVGGGQLGRMLALAGTPLGMRFVVADEHADAPASSVAEVV
                     CGDPSDPATLARLAARVDVVTFENEHIDSGALADLPVPVRPSPGALGIVQDRLTQKQM
                     FMRLGIRTAPFAAVSTAGELPDALAVTGAAAVIKTRRGGYDGRGQAVIERASDAAAAW
                     DAVGRRPAIVESRVRFRRELSVVAVRGEDGRIEIYDPAENRHENGILRVSVAPAPIDE
                     RTRAEAAAAVRAIGEHLGCIGAFALEMFDTPAGLVANELALRVHNTGHWTIEGAETSQ
                     FENHLRAVAGLPLGSAASLGHRAIVNLIGDLPDPAEVLALPSVRLHVYGKTVRPGRKV
                     GHVSVGAADPAGLAERLASLRHLPGAS"
     gene            complement(7259..>7721)
                     /gene="purE"
                     /locus_tag="VM841_01465"
     CDS             complement(7259..>7721)
                     /gene="purE"
                     /locus_tag="VM841_01465"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVL88880.1"
                     /translation="SDWETMKHAADTLESLGVPHEARVVSAHRTPDALFAYAQSAAER
                     GLAAIIAGAGGAAHLPGMLAAKTAVPVLGVPVRSEALRGMDSLLSIVQMPAGIPVATF
                     AIGRAGAVNAALFAAAMIARGSPPVAAALDTYRARQTETVARSADPREEAP"
BASE COUNT         1199 a         2692 c         2701 g         1129 t
ORIGIN      
        1 ggcattcgca atcccgacat cgtcgcgccg ggccgctcgg tgcaaagcct gcgctccgcg
       61 ggctcgttca tcgaccaaac ctacccgggc ggcattatcg gcggccggtt cttccgcggc
      121 agcgggacct cacaggccgc cgcggtggtg tcgggcgcgg cggcgctgct tctgcagcag
      181 cgtcccgagc tgtcgcccga tcagctcaag cacatcatga agtcgacggc aaccaccttg
      241 ccggcggccg acccgatcgc ccagggccac ggaatgctcg acctgcggtc cgcctcgcgg
      301 gcgatgacgc cgctcagcat cgtggccgct caggcgcttc cgtcggcgac cggcactggc
      361 tcgctcgacc tcgcacgcgg cagcgcccgt gtctcgatgg acggcgtgat cctcaatggc
      421 gagcgcgaca tcttcggccg ggcgtggaac ggcgcagcgt gggcgccggc cgcattggcc
      481 gagacgtcct ggaacggtgg cacctggaac ggcgcgacgt ggagcggcgc atcgtggagc
      541 ggcgcatcgt ggagcggcgc atcatggagc ggcgcatcat ggagcggcgc atcatggagc
      601 ggcgcatcat ggagcggcgc atcatggagc ggcgcatcat ggagcggcgc atcatggagc
      661 ggcgcgacgt ggagcggcgc atcatggagc ggcgcgacgt ggagcggcgc aacgtggagc
      721 ggcgcaacgt ggagcggcgc atcatggagc agcgcaggct ggtcctgacg cgccaaagaa
      781 agcatcggat cccggagacg gatggtgtct ccgggatcga tgcgtctacg gagttcggcc
      841 ggtgagcgcg atgagcttcg tctgcggatc ggcgtcgtcg ggaaccagga ccgcagccgc
      901 gaacacaccc gcctctcggg ctccttcgat gaccggcgtg agatagccgg tcgcaagatc
      961 caccagcacc gggtcgagcc gctcatcggc cccgaccgcc cgcgcgaggt cccacgtgtg
     1021 cacggtgatg tcggccgtga cctcggtcac gtagccggcc gtgtcgcgct ccccgtagga
     1081 gagctgcacc gggcgctcca gcgctccggg ctgcgacagg atcgacagaa cccgttcggt
     1141 cgccgcgtcc cacgccgcga ccgcgtcgtc gcccagcagg tcgccgtcaa gcgacggacc
     1201 gacctccgcg atcgtctttc cttcgacgag cggaggaatc cacatgatct cgttgacgac
     1261 gtggttggac agcgcccggg catcccactc ggtgcacggc gtgtcgaccg tccagtcggt
     1321 caccttctcg agccgcgagc ggaactcggc ggccgcccgt tccagcagct tgatcgtgtc
     1381 catgatccga ccgtacgccg cgagccgtgg aaaaagcaag ggtgcctgcc cggcaatccc
     1441 ccgcgaagct ggattaggct tcccgcatga gcgacctgac gttgatggcg gtgcacgccc
     1501 acccggacga cgaggcgatc gggaccggcg ggctgctggc acggtgcgcg gccgaagggg
     1561 tccgcacggt gctgatcacg tgcaccaacg gcgagctcgg ggacctgccg ggcggcgtca
     1621 aacccgacca cgaggaacac gacgttgcgg ccgtagttgc cgcgcgccgc gaggagcttc
     1681 gacgctcctg cgacatcctc ggtgtgtcgc acctcgagat gctcggatac cacgattcgg
     1741 ggatgatggg ctggccccag aacgagcatc cgcacgcgtt ctggcacacg ccggtggacg
     1801 aggcggccgc gaagatcgcc gcgctgatgg agacctaccg gccgcaggtc gtggtcacct
     1861 acgacgagaa cggcttctac ggccacccgg accacatcca ggccaatcgc gcgacgctcg
     1921 ccgcggcggc gatgtgcggt gtcccgcgca agatctacta caccgcggtc agcaggcagt
     1981 ccatcgggcg cctcgcgcgg ctcgtgcgcg acacgatgcc cgaccgcgcc ggcgactggg
     2041 agttcgatcc cgacgatccc ccgttcggga tcgacgaggc cgagatcacc acggtgctgg
     2101 acgtgagcga gttcatccgc cgcaagcgcg acgccatggc cgcccacgcg agccagtccg
     2161 aaaacatcga gttcctgcgc atgcccgacg agctgttcgg tcaggtcttc ggcgcggagt
     2221 cgttcgttcg cgtgcgcgac gacaccggcg ctccgctccc cgagaccgac cttttcgccg
     2281 gccttcgcta gagccggcgc accaggtccc gacggcccgg acccccgatc cttggggggt
     2341 tcattgtcga ccaacgtcac tggcctcgtg ccggcccggt tccgatactc cgggggtgag
     2401 gcatcccgtc cgccgtccgg aggtcgccat gaagatcacg ctcggggtca gctggaacga
     2461 cataaaggcg tgtaccgccg ctctcctcgc gctcgtcgtg ccgctcgcgc ttcggcggcc
     2521 tgcgagggcc ggcacctgac cgtcatgaac acgacgccgg ccaccgagtc gccggggcgc
     2581 gggccggggg cgccgggggc tcgcttcacc ggtgcgcagc gcgtctggat cctcaccggc
     2641 atcatcgcgg tcatctcgct cgcgatgtac gtgggactgg tgctcggaca cgagccgatc
     2701 aagccctcgt acgagattcc ctggtgggtg ctcgcggccg ggttcttcgc cgcggagatc
     2761 ttcgtcgtgc acatccagct ccgccgggac gactactcgt tctcgctgag cgagatcccg
     2821 ctcgtgctcg gcctgttctt cgccagcgcg gaggcggtga tcttcgcgca cgtcgtgggt
     2881 gcgggcgccg cgctgatcgt gcatcgccgc cagtcgctgc tcaagctcgc cttcaacgtc
     2941 tcgcacttct tgctggaggt cagcctcgcg gtcgcggcct tccggctgtt cgccggggtt
     3001 caggtgaccg tcggcgtgat ggactcgatc gccgcgatgc tcgtcacgct gatcgcgacc
     3061 tgggccgcgc tgttcatgat cttcaccgcg gtcgccatgt cgcagggctc gcgtaacctg
     3121 accggcctgt cgcgcgcgct ggtgctgggg tcggtcacga cgctgacgaa ctcgagcatc
     3181 gggctcgtcg gcgtcacgat cctgggcagc tcgcccgcat ccatctggct tctggccgtg
     3241 ccgtcgatca cgctgttcat cgcctaccgc gcatacacct ccgagcagac gaagcacgag
     3301 agcatcgagt tcctctacca gtccacgagc atcctgcaca aggcgcagga gatggagtcg
     3361 gcgatgctgt cgctgctcga gcagacgcgg cagatgttcc gcgccgagat cgcacagatc
     3421 gtgctgctgt cgagcgacgg gcaaaagccg ctgcgaacga cgctcggccc gggagagacg
     3481 gccgagatca tgcgtcccga cggtcagccg gccggggtgt tccggatggt ggtcgacgag
     3541 cgccgcggcg tgcgcctgag ccgcccgatc cgcagcgacg acttccgcgc gcgcttcgca
     3601 ccgacccagg tcaaggacgc gatgatcgcc ccgctccgcg gagagaccca cgtcctgggg
     3661 cagctgatcg tcgtcaatcg cctcggggac gtcaccagct tcgacgacga agacctcaag
     3721 ctgttcgaga cgctcgccaa ccacgcgagc atcgcgctgg agaacggccg gctcgagaag
     3781 tcgctcgccg aggtgacacg gctcaaggag gagctgcgcc accaggccta ccacgatcct
     3841 ctcaccagcc ttgcgaaccg gtcgctgttc ctggaccggc tgcggcacgc gctcgcgcgc
     3901 cggcgcaccg cgcatcgcga ggtcgccgtg atcttcgtag atctcgacga cttcaagacg
     3961 gtcaacgaca gcttggggca cgcggccggg gatcagctgc tgtgcggtgt ggccgatcgg
     4021 ctgcgcggca tctgccgccc ggccgacacg gtcgcacggc tcggcggtga cgagttcgcg
     4081 atcctgctcg aggagtccac accggtcgag acgatcgcgg tggccgagcg gctgatcgac
     4141 gagctcgaga cgccgtttga gctcgcgcac aaggagatct acgtgcgcgc cagcgtgggc
     4201 atcgcctcgc atggccacgc cggcgacgcc gagtcgctga tgcgcaacgc cgacgccgcg
     4261 atgtatcagg ccaagagcca tggcaagggc cgcttcgagc tctacgaccc gagcatgcaa
     4321 aaggcggcgc tcaaccgcct cgagatgaag gggcggctgc agcgcgccgt cgaacgcgac
     4381 gagatcgaga tcttctacca gcccaccatc gcgctcgcga ccggaaacat tcacggcgcc
     4441 gaggcgctcg tgcgctggcg gcagccgggc tcgcggcggc tcacgcccgt cgagttcatc
     4501 ccgctcgccg aagagaccgg cttgatcaac gtcgtcggac ggcacgtgct ggagcaggcg
     4561 tgcctgcgga tgcgcgaatg gaccgagcgc tatccggtgc tgggaccgct taagatcgcc
     4621 gtcaacatct cggctcgcca gcttcagcag ccggatctgg tcgagatcgt cggggaggcg
     4681 gtgtccgccg ccgggctgag cccctcgcag ctcgtgctcg agatcaccga gagcgtgctg
     4741 ctgcaggaca ccgacgccac gatccggctc ctgcaggagc tcaaggcgct caacgtccag
     4801 ctcgcgatcg acgacttcgg caccgggtat tcgtcgctga gctacctgca gcgcctgccg
     4861 atggacatca tcaagatcga caagtccttc gttcacggcg tcggcgggtc ggccgaggac
     4921 gaggcgctca cgcgcgccat cgtcaagctc gcgcaaacct tccgcctcaa ggtcgtcgcc
     4981 gaaggaatcg agcgccagga gcagatggat cgcctccgcc agatgcgctg ccatctgggc
     5041 cagggctatc tgttctcccc gcccgttccg gcgcagcgct tcgaagagct gctcggcgcg
     5101 gccgtgggag gggccgacgc ggagcttatc gacttcgcga ccgcggtcaa gagcctgccc
     5161 ggccgcgccc cggcgacgag cgctgcgcgt tcggacgcga tggcgaagga cgcgttgccc
     5221 tacgacgcga cgcgatagga cacgcgacac acgccgcggc tcgtcgagcc gcacacgcct
     5281 cggaacgcgg cagccgacag gtcgagcccg cggccggcga cgtacggccc gcggtcgtag
     5341 acgcgcaccg tcatcgttcc cgcgggtccc gtgaccgcga ccgcggtgcc gcacggaagc
     5401 gtcttgtgcg cggtccacag ggtcaggccc ttcgggaagg cgtgacggcc gcgacgatcc
     5461 cagcacgccg aaggccggtc gttgtaccac gaggccagcg tgccgccgcc accggacgcc
     5521 gacgcggccc ccgacgaagc ggcggtcggc ggcgaggatc gcgcggagcg agcagcacgc
     5581 gactcgacgc gccgcgggac ggcctttgcc cgctcgcgag cctcacgcgc gcgagcgaga
     5641 tccgccggac ggatagcgat cttcacgggc tcgatcacgc ggagcgcgga cagtccgatc
     5701 cggaccgggc cgtccacgcc cgtggcgacg gcggcgggct cggtggacag cagcgccgcg
     5761 agccggccgc ccgccaggtg gtcgggtacg ggtgcgggcg ccggatcggg cgaggcaccg
     5821 gacggcagcg cggtcaatgg cagcgcgacg gcggcaatgg cggtgatggc gagcttcttc
     5881 atgggtgtcg ctcctccgtg ccccgggcac gacggcgcga tcgcatgcgt ggggcatgca
     5941 tgagcgaacc acgaggggat tcggccggat cggcgctgtc gtatccgcga ggcggacgac
     6001 gcggctccgc atgagacgcg gaggctattt atggatgtgt tcgtctacgg gtacagcacc
     6061 ctggtgggaa catcaaattg agggtgtttc cgatggcgta atacgtcgtt gtatttccag
     6121 gcatacccgg cgaagttttt cccgaaaccc tcgcggcgac gaatgaagac gttcggtcag
     6181 ctcgcgcccg gcaggtgacg cagcgaagca agccgctcgg ccagcccggc cgggtcggcc
     6241 gccccgaccg acacgtgccc gaccttgcgg ccgggacgca cggtcttgcc gtatacgtga
     6301 aggcgcaccg acgggagcgc cagaacctcc gcgggatcgg gcagatcacc gatgagattg
     6361 acgatggcgc ggtggccgag cgacgccgca cttccgagcg ggaggccggc caccgcacga
     6421 aggtggtttt cgaactggct cgtctcggcg ccttcgatcg tccagtgccc cgtgttgtgg
     6481 acgcggagcg cgagctcgtt ggcgacgaga ccggcgggcg tgtcgaacat ctcgagcgcg
     6541 aatgcgccga tgcagccgag atgctcgccg atggcgcgta cggcggccgc cgcctcggcg
     6601 cgggtgcgct cgtcgatcgg cgccggcgcg acgctcacgc gcaggatgcc gttctcgtgt
     6661 cggttctcgg ccggatcgta gatctcgatg cggccgtcct cgccgcggac cgcaacgacg
     6721 gacagctcac gtcggaagcg cacgcgcgac tccacgatcg cggggcggcg gccgacggcg
     6781 tcccaggccg cggccgcgtc gctcgcgcgt tcgatgaccg cctggccccg tccgtcgtag
     6841 ccgccgcgcc gggtcttgat cacggccgca gcgccggtga ccgccaaagc gtccggcagc
     6901 tcgccggcgg tcgacaccgc cgcgaacggc gcggtgcgga tgccgagccg catgaacatc
     6961 tgcttttgcg taagacgatc ctgcacgatg ccgagcgccc cgggggacgg gcgcaccggc
     7021 accggcagat ccgccagcgc accggaatcg atgtgctcgt tctcgaaggt cacgacatcc
     7081 acgcgggccg cgagccgcgc gagcgtggcg ggatcggacg gatcaccgca cacgacttcg
     7141 gccaccgacg atgcgggcgc gtccgcgtgc tcgtcggcga cgacgaaacg catgccgagc
     7201 ggcgtgcccg cgagcgcgag catccggccg agctggccgc cccccacgat tccgatcctc
     7261 atggcgcctc ctcgcgtggg tcggcggacc gtgcgaccgt ctcggtctgc cgcgcgcggt
     7321 acgtgtcgag cgccgcggcc accggaggcg agccgcgtgc gatcatcgca gcggcgaaca
     7381 gcgccgcgtt gaccgcgccg gcgcggccga tcgcgaaggt ggcgaccgga atcccggcgg
     7441 gcatctgtac gatcgaaagc agcgagtcca tgccgcgcag cgcctcggat cgcaccggaa
     7501 cgccgagcac cggcaccgcc gtcttggcgg cgagcatgcc cggcaggtgc gcggcgccgc
     7561 ccgcgcccgc gatgatcgcc gccaggccgc gctccgctgc cgattgggcg tacgcgaaca
     7621 gcgcgtcggg cgtgcggtgc gcggaaacaa cgcgcgcctc gtgcggcacg ccgagcgatt
     7681 cgagcgtgtc cgcggcgtgc ttcatcgtct cccaatcgga g
//
LOCUS       DATLFO010000044         7543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_31409, whole genome shotgun sequence.
ACCESSION   DATLFO010000044 DATLFO010000000
VERSION     DATLFO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31409"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VM841_01470"
     CDS             <1..1190
                     /locus_tag="VM841_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL88881.1"
                     /translation="ADGDGEHAAGGVLAVAVRDADDAETVRLLAAYATEAGLAIANAV
                     LHEQVRLKEEELETIVEAMASPIVLVDQRGVFRAMNPAAAETFHLTPEFEIGQTAHGK
                     LGEQIEELLLGQAEGTREIVLPVGGDPHVWRATVTTTRNGPVPGGRLLVLDDVTLQRE
                     LEQRKADFLAVIGHELRTPLTIIRGFATTLARRDLELDDGTRTTAVGTIMEQSERLGR
                     LIEDLLYVSKIENRTPPLHLEWNDVVQTVNDVIAAMREREPEWPISLRNSATSVHLLF
                     DRVKVEQVLGHLLDNAVKYSEPQAPIAIRVDDEGGDDVRITVEDKGVGIFSGDIPRLF
                     RVFGQLDASSTRRAGGTGVGLSVCRTLVEAMGGRIWAQSMLGKGSEFSFTVPKTPPAS
                     QRA"
     gene            complement(1187..2164)
                     /locus_tag="VM841_01475"
     CDS             complement(1187..2164)
                     /locus_tag="VM841_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVL88882.1"
                     /translation="MPPTGSIPRLSRILAVVPWVINHPEQATVEEVCRRFGLSRDQLI
                     DDMALLTMCGLPPFTPGDLIEAWVEDERVVISMADYLDKPLRMSRWEALGLLAAGRAL
                     AALPALTEAQALERALAKLAEAIAPDEADAAATLAERVAIELQPASPEILAALRDAIE
                     ERRRLRIEYYSFGRDELTERTVSPLLVFGTGPWYLIADDDRSGERRTFRVDRIRHAQP
                     TGDTFERPLGFDPTREADQPLYRPSPGDVKVVLELSPAASWVAELTPHEEAKPVKGGW
                     TRMTLRASHTAWLARLVLQLGGDVRVIEPDTLAAEAGDLATAALVRYGV"
     gene            complement(2164..3096)
                     /locus_tag="VM841_01480"
     CDS             complement(2164..3096)
                     /locus_tag="VM841_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVL88883.1"
                     /translation="MDKTERQINLIALLMSSERPVSGARIREGLYADATSDESFKRMF
                     ERDKDDLRALGLRIEIAPIGVWDEGYTIHRDEAILPPLDLTPAEHAALMLASQAWGEG
                     TLGPASPRAAGMKLSVTGDGDGPSPWILPHVDLRPPQLTAVFDALMRRKRVTFAYRSS
                     GAAVPEERTVEPHGLRFRSAWYLTGLDIGRGELRVFKLDRIDGAVRIAAGSKPDFQAP
                     PPAPVEWPAEAEAGLQARVAFTPEVAFLVEQRAGARRAGDRDDGRVDVLVAVPDEDRF
                     VRWVLGFADDAEVIEPPSLRDVTIERLRAAAGKA"
     gene            3149..4204
                     /locus_tag="VM841_01485"
     CDS             3149..4204
                     /locus_tag="VM841_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HVL88884.1"
                     /translation="MIRIRRGLVRQVRERHAGLLDVEVEIDGTTEAAVAYPDLCGPVT
                     PGDRVVLNTTAVSLGLGTGGVHFVIAVEGGSDLDPGDGVHAIKLRYTPVQVAVRAIEE
                     GGAPLPEDLDGMPVVAAGLHSAIAPVAIAAHAISPGIRIGLVITDAAALPLAFGDSVP
                     ALRAAGLIAATVTCGQAFGGDVEAINKYSGMLAARAAGAQMIIVAMGPGNLGTGSRYG
                     FAGMEVAEVVNAVAALGGSPVAVPRIGFGDPRDRHRGVSHHTITALGDAAFASAIVAL
                     PPYGGETGAAVRGALAPLARRHRIREIDLGPAEQALRDAPVPMRTMGRSFDEEPEYFR
                     TAAAAGVLAARMAAGEQ"
     gene            complement(4210..5568)
                     /gene="pafA"
                     /locus_tag="VM841_01490"
     CDS             complement(4210..5568)
                     /gene="pafA"
                     /locus_tag="VM841_01490"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HVL88885.1"
                     /translation="MDRRIYGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDDIVELVAHDKAGERILEGLLAAAERRLHDEGI
                     SGDIYLFKNNTDSAGNSYGCHENYLVSRYGEFQKLADILIPFLVTRQIYAGAGKVLQT
                     PRGPLFCLAQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEY
                     ATLMKVGTTDLVLRMVEEGTVMRDLTLENPIRAIREISHDIGCTRRVKLASGREMSAL
                     EIQHEYFDRAKKFVERRGIDGFATRVLGMWEEALTALAAGDLAALDGKLDWVTKHRLV
                     EAYRKKHNVSLAHPRVALLDLAYHDVNRERGLYYLLERKGKMVRTVDEATIAEAMKEP
                     PQTTRARLRGEFIKTAKERRRDFTVDWVHLKLNDQAQRTVLCKDPFKAHDERVEKLIA
                     SM"
     gene            5619..6236
                     /locus_tag="VM841_01495"
     CDS             5619..6236
                     /locus_tag="VM841_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88886.1"
                     /translation="MDGPRPDETAGRVRPVTRMLLVLGALLVIFAAVLAVELITGERT
                     PEAGAELLAAPDPVIPRSIAGYTIRDEPGLASTLTQEYRARSLAVERIEAASIPLRAS
                     ASSLLAATMKAEANLASSQFRAGVLTGAASGFGIDPSQHPFRYRVAGGVVVYTTEAPE
                     GALSVWFFRDAFVQLFVPKELVSEAPEIQRVVLEAQLVQVDAARR"
     gene            6248..7324
                     /locus_tag="VM841_01500"
     CDS             6248..7324
                     /locus_tag="VM841_01500"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HVL88887.1"
                     /translation="MLGVIDFHAYPRIGGLSPHGVGIAVGYLLGGMLMVRYAAKRGLP
                     ADEVWNMLMRAVFGVIIGARLFYVAGHLDEFFGAGRSPLDILKVWQGGIVFYGGAVGG
                     IVAAIPYIRKHGYPMRTILDLTAAAFPLGLIFGRIGDIIVGDHLGGASTLPFAMRFFP
                     YEVPPNPGAYVAHSFSSEGDFVACFTSGCHQTALYDLVNVLILLPVVMLLARKPRPAG
                     WLFAFMVTWYGGARLVTDFARDAQYYGIGPVQLHGTQWFSILLIVAGSIYLLRLARRG
                     EPDTWMYTPPGEPAAEGVAEPAAVAATAASLPESPTGLDTGGPVASEPEPGATPEPVR
                     EPEPVREPEPPGEQEPGGGTRTDR"
     gene            complement(7325..>7543)
                     /locus_tag="VM841_01505"
     CDS             complement(7325..>7543)
                     /locus_tag="VM841_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88888.1"
                     /translation="VWDSAGEARQAADALTDWMRPRFRGEGRSYRDGSVAGWQAPRGA
                     AEVLRDGARVVLVVGPDRESVSKARRAF"
BASE COUNT         1053 a         2677 c         2665 g         1148 t
ORIGIN      
        1 tcgcggacgg cgacggcgag cacgccgccg gcggcgtgct cgccgtcgcc gtccgcgacg
       61 ccgacgacgc cgaaacggtc cggctcctgg ccgcctacgc gacggaggcg ggcctggcga
      121 tcgccaacgc ggtgctgcac gagcaggtcc ggctcaagga ggaggagctc gagaccatcg
      181 tcgaggcgat ggcgagccct atcgtgctgg tggaccagcg aggcgtattc cgcgcgatga
      241 acccggccgc cgccgagacc ttccatctga cgccagagtt cgagatcggg cagacggcgc
      301 acggcaagct cggcgagcag atcgaggagc tgctgctcgg ccaggccgaa ggcacgcgcg
      361 agatcgtgct gcccgtcggc ggcgacccgc acgtgtggcg cgcaaccgtg accacgaccc
      421 gcaacggtcc cgtccccggc ggacggctcc tggtgctcga cgacgtgacg ctgcagcgcg
      481 agctcgagca gcgcaaggcc gacttcctcg ccgtgatcgg acacgaactt cgcacgccgc
      541 tcacgatcat ccgcggcttc gcgaccacgc tcgcccggcg cgacctcgag ctcgacgacg
      601 gcacgcgcac gaccgcggtc ggcacgatca tggagcagtc cgaacgcctg ggccggctca
      661 tcgaagacct gctgtacgtg tcgaagatcg aaaaccgcac tcccccgctg cacctggaat
      721 ggaacgacgt cgtgcagacc gtcaacgacg tcatcgccgc gatgcgcgag cgcgagcccg
      781 agtggccgat ctcactgcgc aactcggcaa cgtcggtgca tctgctgttc gaccgggtca
      841 aggtcgagca ggtcctgggg catctgctcg acaacgcggt caagtactcc gaaccgcagg
      901 ccccgatcgc catcagggtc gacgacgaag gaggcgatga cgtccgcatc accgtcgagg
      961 acaagggcgt gggcatcttc tcgggcgaca tcccgaggct gttccgcgtc ttcggccagc
     1021 tcgacgcatc ctcgacgcgg cgcgcgggag gcacgggcgt ggggctgtcg gtgtgccgca
     1081 cgctggtcga ggcgatgggc gggcgtatct gggcgcagag catgctcggc aagggctcgg
     1141 agttctcgtt caccgtgccg aagacgccgc cggccagcca gcgcgcctag acgccgtagc
     1201 ggacgagcgc ggcggtcgca agatcgcctg cctcggccgc gagcgtatcc ggctcgatca
     1261 cgcgaacgtc cccgccgagc tgaagcacga gccgcgcaag ccaggcggtg tggctcgcgc
     1321 gcagggtcat gcgcgtccac ccgcccttga ccggcttggc ctcctcgtgc ggcgtgagct
     1381 ccgcgaccca ggacgcggcg ggcgagagct cgagcaccac cttcacgtca ccgggtgaag
     1441 gacggtacag cggctggtcg gcctcgcgcg tcgggtcgaa cccgagcgga cgctcgaacg
     1501 tgtccccggt cggctgggcg tgacggatcc ggtctacccg gaacgtgcgc cgctcccccg
     1561 accggtcgtc gtcggcgatc aggtaccacg gcccggttcc gaacaccagc agcgggctca
     1621 cggttcgctc ggtgagctcg tcgcggccga acgagtagta ctcgatgcgc aggcgccggc
     1681 gttcctcgat cgcgtcgcgc agcgccgcga gaatctcggg cgaggccggc tgcagctcga
     1741 tcgcgacccg ctcggcgagc gtggccgcgg catcggcctc gtcgggcgcg atcgcctcgg
     1801 cgagcttggc gagcgcgcgt tcgagcgcct gcgcctcggt gagcgcgggc agggcggcga
     1861 gcgcacggcc cgcggccagc agccccagcg cctcccaccg gctcatgcga agcggcttgt
     1921 cgagataatc ggccatgctg atcacgaccc gctcgtcctc gacccaggcc tcgatcaggt
     1981 cgccgggcgt gaacggcggc aggccgcaca tggtcagcag cgccatgtcg tcgatgagct
     2041 ggtcgcggct gagcccgaag cggcggcaca cctcctcgac cgtggcctgc tcgggatggt
     2101 tgataaccca gggcacgacc gccaggatgc ggctcaggcg cgggatcgat ccggtgggcg
     2161 gcatcaggcc ttgcccgccg cggcacgcag ccgctcgatc gtgacgtcgc gcagcgacgg
     2221 cggctcgatg acctcggcgt cgtcggcgaa gccgagcacc caccgcacga accggtcctc
     2281 gtcgggcacc gccacgagca catccacgcg cccgtcgtcg cggtctcccg cgcggcgcgc
     2341 gccggcccgc tgctcgacca ggaacgcgac ctcgggcgtg aacgcgactc gcgcctgaag
     2401 gccggcctcc gcctcggccg gccattcgac gggcgcaggc ggcggcgcct ggaagtccgg
     2461 cttggatccg gcggcaattc ggacggcgcc gtcgattcgg tcgagcttga acacacgcag
     2521 ctcgccgcgg ccgatgtcga gcccggtcag gtaccaggcg gagcggaagc gcaggccgtg
     2581 cggctcgacg gtgcgctctt cggggacggc cgcgccggag gagcgatagg cgaaggtgac
     2641 ccgcttgcgg cgcatcagcg cgtcgaacac cgcggtcagc tgcggcggcc gcaggtccac
     2701 gtggggcagg atccacggcg agggcccgtc gccgtcgccc gtcaccgaca gcttcatgcc
     2761 ggccgcacgc gggctggcgg gtccgagcgt gccttcgccc caggcctgtg aggcgagcat
     2821 cagcgcggcg tgctcggcgg gcgtcaggtc cagcggcggc aggatcgcct cgtcgcggtg
     2881 gatcgtgtat ccctcgtccc agacgccgat cggggcgatc tcgatccgaa ggccgagcgc
     2941 gcgcaggtcg tccttgtcgc gctcgaacat gcgcttgaac gactcgtccg aggtcgcgtc
     3001 tgcgtagagg ccctcgcgga ttcgcgcgcc ggaaaccggc cgctccgacg acatcagcag
     3061 cgcgatgaga ttgatctgcc gctcggtctt gtccatgccg cccaaagcgt acccgcaccg
     3121 cgggcggagg cgggtaggct ttgcggccgt gatccggatc cggcggggcc tcgtccggca
     3181 ggtgcgcgag cgccacgccg gcctgctcga cgtcgaggta gagatcgacg gaaccaccga
     3241 ggccgccgtg gcctatccgg acctgtgcgg gccggtcaca cccggcgacc gcgtggtgct
     3301 caacacgacc gcggtctcgc tcgggctcgg caccggcggc gtgcatttcg tgatcgcggt
     3361 cgagggtgga tccgacctcg atcccggcga cggggtgcac gcgatcaagc ttcgctacac
     3421 gccggttcag gtcgcggtcc gcgcgatcga ggagggcggc gccccgctgc ccgaggatct
     3481 cgacggcatg ccggtcgtcg cggccgggct gcacagcgcg atcgcgccgg tcgcaatcgc
     3541 cgcgcacgcg atctcccccg ggattcgcat cgggctcgtg atcaccgacg cggccgcgct
     3601 tccgctcgcc ttcggcgact cggtgcccgc gctgcgcgcc gcaggcctga tcgccgcgac
     3661 ggtgacgtgc ggccaggcgt tcggcggcga cgtcgaggcc atcaacaagt attcgggcat
     3721 gctggccgcg cgcgcggcgg gcgcgcagat gatcatcgtg gcgatggggc cgggcaatct
     3781 cggcaccggc tctcgttacg ggttcgccgg aatggaggtg gccgaggtgg tcaacgccgt
     3841 cgccgcgctc gggggctcgc cggtcgcggt gcctcgcatc gggttcggcg atccgcggga
     3901 ccggcatcgc ggagtgtccc accacacgat caccgcgctc ggcgacgccg cgttcgcgag
     3961 cgcgatcgtg gcgcttccgc cctacggcgg cgaaaccggt gcggccgtgc gcggcgcgct
     4021 cgcgccgctg gcgcgccggc atcgcatccg cgagatcgat ctcgggccgg cggagcaggc
     4081 cttgcgggac gcgccggttc cgatgcgcac gatgggccgc tccttcgacg aggagcccga
     4141 gtacttccgg accgcggctg cggccggcgt gctcgccgcg cgcatggccg ccggcgagca
     4201 gtagccccgc tacatcgagg cgatcagctt ctcgacccgt tcgtcgtggg ccttgaacgg
     4261 gtccttgcac agcacggtcc gctgcgcctg gtcgttgagc ttgaggtgca cccagtcgac
     4321 ggtgaagtcg cggcggcgct ctttggcggt cttgatgaac tcgccgcgca gccgcgcgcg
     4381 cgtggtttgc ggcggctcct tcatcgcctc ggcgatggtg gcctcgtcca cggtgcggac
     4441 catcttgccc ttgcgctcga gcaggtagta cagaccgcgt tcgcggttga cgtcgtggta
     4501 ggcgagatcg agcagcgcga cgcgcggatg cgcgagcgag acgttgtgct tcttgcgata
     4561 cgcctcgacg aggcgatgct tcgtgaccca gtcgagcttt ccgtcgagcg cggcgagatc
     4621 gccggcggcg agcgcggtca gcgcttcctc ccacatgccg agcacgcgcg tcgcgaatcc
     4681 gtcgatgccg cggcgctcga cgaacttctt ggcgcggtcg aaatactcgt gctgaatctc
     4741 gagcgcgctc atctcgcgtc cgctggcgag cttgacgcga cgcgtgcagc cgatgtcgtg
     4801 gctgatctcg cggatcgcgc ggatggggtt ctcgagggtg aggtcgcgca tgaccgtgcc
     4861 ttcctcgacc atgcgcaaga ccagatcggt ggtgccgacc ttcatcagcg tcgcgtactc
     4921 gctcatgttc gagtcgccga cgatcacgtg gagccggcgg tagcgctccg cgtcggcgtg
     4981 cggctcgtcg cgcgtgttga tgatcggccg tgagcgggtc gtggcgctcg agacgccttc
     5041 ccagatgtgc tcggcgcgct gcgcgaggca gaacagcgga ccgcgcggcg tctggagcac
     5101 cttgcccgcg cccgcgtaga tctggcgcgt caccaggaac ggaatcagga tgtcggcgag
     5161 cttctggaac tcgccgtagc gcgagacgag atagttctcg tggcacccgt aggagttgcc
     5221 cgccgagtcg gtgttgttct tgaacaggta gatgtcgccc gagatcccct cgtcgtgcag
     5281 gcggcgctcc gcggcggcga gcagcccttc caggattcgc tcgccggcct tgtcgtgcgc
     5341 gaccagctcg acgatgtcgt cgcattcggg cgtggcgtac tcgggatggg atccgacgtc
     5401 caggtacagc cgcgcgccgt tttcgaggaa cacgttcgac gagcggcccc aggacacgac
     5461 ccggcggaac aggtaccggg caacctcgtc gggcgacagg cgtcgctgtc cgcggaacgt
     5521 gcaggtgacc ccgtattcgt tctcgagccc gtagatgcga cgatccatag gagcgccact
     5581 gtaccgcgtg cgcagccgct gccgggtacg atcgggcgat ggacggtcct cgccccgacg
     5641 aaacggccgg ccgcgtcagg cccgtgacgc gcatgctgct cgtgctgggc gctctgctgg
     5701 tgatcttcgc cgccgtcctt gcggtcgagc tcatcacggg tgaacggacg cccgaagcgg
     5761 gggccgagct gcttgccgcg cccgacccgg tcatccccag aagcatcgcg ggctacacca
     5821 ttcgtgacga accgggcctc gcctcgacgc tgacgcagga gtaccgggct cggtcgctgg
     5881 cggtcgagcg cattgaggcc gcctcgatcc cgctgcgcgc ctcggcgtcg tcgctgctgg
     5941 ccgccacgat gaaggccgag gccaacctgg cctcctcgca gtttcgtgcc ggcgtcctga
     6001 ccggagccgc ctcggggttc gggatcgacc cctcgcagca ccccttccgc taccgcgtcg
     6061 cgggcggcgt cgtggtctac acgaccgagg cgcccgaagg cgcgctctcc gtctggttct
     6121 tccgcgacgc cttcgttcag ctgttcgtcc cgaaggagct ggtgtcggag gcgccggaaa
     6181 tccagcgggt cgtgctcgag gcccagctcg tgcaggtgga cgcggctcgc cggtaggatt
     6241 cgcgccgatg ctcggggtca tcgacttcca cgcctatccg cggatcggcg gcctctcgcc
     6301 gcacggagtc gggatcgccg tcggctatct gctcggcggc atgttgatgg ttcgctacgc
     6361 cgccaaacgc gggctgccgg cggacgaggt ctggaacatg ctgatgcgcg ccgttttcgg
     6421 cgtcatcatc ggcgcgcggc tcttctacgt agccggacac ctggacgagt tcttcggagc
     6481 gggccgaagc ccgctcgaca tcctcaaggt ctggcagggc gggatcgtct tctacggcgg
     6541 cgcggtcggc ggcatcgtcg ccgcgatccc ctacatccgc aagcacggct atccgatgcg
     6601 cacgatcctc gacctcacgg ccgcggcgtt tccgctcggc ctgatcttcg gccgcatcgg
     6661 cgacatcatc gtcggcgacc acctgggcgg cgcctccacc ctgccgttcg cgatgcgctt
     6721 cttcccgtac gaagtgccgc cgaacccggg cgcctacgtg gcgcactcgt tctcgagcga
     6781 gggggatttc gtcgcgtgct tcaccagcgg ctgccaccag accgctctct acgatctggt
     6841 gaacgtgctc attctgctgc ccgtcgtgat gcttctcgcc cgaaagccac ggccggccgg
     6901 atggctcttc gccttcatgg tgacctggta cggcggcgcg cgtctggtca ccgacttcgc
     6961 ccgcgacgcc cagtactacg gcattggccc cgtgcagctt cacggcaccc agtggttctc
     7021 gatcctgctc atcgtggccg gctcgatcta cctgttgcgc ttggcacgcc gcggcgagcc
     7081 ggacacgtgg atgtacacgc cgccggggga accggcggcc gagggcgttg ccgagccggc
     7141 cgccgtggcc gcgaccgcgg cctcgcttcc cgagtccccc accggcctgg acaccggagg
     7201 tccggtcgcg tcggaaccgg agcccggcgc gacacccgag ccggtgcgcg aacccgagcc
     7261 ggtgcgcgaa cccgagccgc cgggcgagca ggagcccggc ggcggcacgc gcaccgatcg
     7321 ctgatcagaa ggcgcggcgg gccttcgaga ccgactcccg gtccggccct accaccaaca
     7381 cgacccgcgc gccgtcgcgc agcacctccg ccgctcctcg aggagcctgc cagcccgcga
     7441 ccgagccgtc gcgatagctg cggccctctc cacggaagcg tgggcgcatc cagtcggtga
     7501 gcgcgtcggc cgcctgtcgc gcctcacccg ccgagtccca gac
//
LOCUS       DATLFO010000045         5557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_100739, whole genome shotgun sequence.
ACCESSION   DATLFO010000045 DATLFO010000000
VERSION     DATLFO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100739"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1289
                     /locus_tag="VM841_01510"
     CDS             342..1289
                     /locus_tag="VM841_01510"
                     /inference="COORDINATES: protein motif:HMM:NF037269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HVL88889.1"
                     /translation="MEEVKAEIGRYVRRHADDPGRLFDADVVVSELLSNAVRHAAGPA
                     WVSIVWTTPRPVLSVYDLGPGFVFDPKLPDDPLAEGGRGLYIVSHMVDELDAASRRAG
                     GTRVEAVLPVTRQSSDTYDPPRRRSGALPHPDEAQPGGGFGKESFLRALVVQLAQAIE
                     ADHGPAAAEAAVAQVAADVGGRMEDEYRAAKDIAGRMTPGQMADCYVRLKHAIDGGFY
                     VIEATEGRIVLGNTACPFGSVVTRAPALCRMTSSVFGGIAARNSEKEASVVLEERIAV
                     GDPGCRVVVYLDRPPDDPGIAHHYATPVDELEPEPEATR"
     gene            complement(1297..2130)
                     /locus_tag="VM841_01515"
     CDS             complement(1297..2130)
                     /locus_tag="VM841_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HVL88890.1"
                     /translation="MTVRIVTDSAATIPAAAAAEHDIDIVPMYLKFGDETYRDGVDIP
                     PGYFYERLQTETVPVSTAGASVGDFHDAFAAALKKADAVVCVTVASFVSVTHTSATIA
                     ARDFGGRVIVVDSASASMGEGFIALEAARAARSGADADAVAARARDVSSRTQVVATIN
                     TFEYLKRSGRVHALMAYAGTALGIKPVFAFRGGKVEQLGRPRSRGKAVDKVADEARTA
                     AAQGPLHLAVCHAAAPDEAAALLDRLTAELNPAEAHLAEFTPLMGAHTGPGLLGAAFW
                     S"
     gene            complement(2127..3629)
                     /locus_tag="VM841_01520"
     CDS             complement(2127..3629)
                     /locus_tag="VM841_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HVL88891.1"
                     /translation="MSLVLALDVGTSGGRALIADSGGKRIGSAERRWRYRTDPDGFPV
                     LDTGSVWASLASATREAVRSSLARPEAIAVIGITAQRNGVALLDSKGQILYAGPDSDG
                     RAANEGIALEREHGDLIYETTGRLPAFKYLPARLAWFRGNKPEIANRTAHALSFGDWI
                     AFRLTGEIATDPTMAAETMCFDVRREMWSQTLCDALDVPSRFLPRVMTEPLGRLPEDV
                     AREFGLHPGTPVVRAGADTQCAALAMGVIEPGDVAINAGTMMPVTQVQGEAIIDAQRR
                     LVTSPHVVPGRSILEVNCGEAGALVDWLLGLIGEAGNHLWLEQSAGESDPGSRGVAIV
                     DPGPINVGDYPVMRSGGLAFPIPVSALNPGREDLSRAVLEGVAYAARAGIEWLQEVSG
                     RDAGLVSVCGGLSRSPTFVRVLAGVLGREVRRAREPHASALGAAIVAATAAGLHPTVA
                     AAAAKMSDQGELVRPDPNWLALYDGLYQSWTGLRARYEETMMSVSDLRDS"
     gene            complement(3718..4578)
                     /locus_tag="VM841_01525"
     CDS             complement(3718..4578)
                     /locus_tag="VM841_01525"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HVL88892.1"
                     /translation="MTDREGFHDRDTDALDRALDAVVAGDEALLPEASMIRAALRREV
                     PDAVARDHLAAIRSAADPQLASPERSPEPDTTKLSSPERYPDAQRRPAADIVPIDDVR
                     KRRVGRGRRRGAVAVLAAAMLLTFGSGSALAAAAGTKPGDALYGVKRASERIALAMRL
                     SPESKAALHLQLAERRIDEIDALAAEGQDVTDLAADFAASLEAAGEGASSEALGRILE
                     RTRAHIAHLNEILNQVPEQAQQGLERAIDNAERQQQRTEERRQQTQDRGKPDAPGRSG
                     DAPGKKNRGR"
     gene            complement(4580..5194)
                     /locus_tag="VM841_01530"
     CDS             complement(4580..5194)
                     /locus_tag="VM841_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL88893.1"
                     /translation="MSGGVDPAAALGRVLAGDRNGFEDLYRVYAGPLLSFLATQVRRT
                     EDAEDLVGQVFLEAMRGIDRFEGDAAAFRAWLFRIGRDRATDLGRRQSRRPEEPLEAA
                     GDAPAEQGTEEQALAALERRRVWEAVAALPDAQREVIALRLGSGLSSGEIAEVVGKDV
                     NAIKALQHRALGNLAKTLGKQMPQIPPTSGPSPYPPAVPRRLEG"
     gene            complement(5191..>5557)
                     /locus_tag="VM841_01535"
     CDS             complement(5191..>5557)
                     /locus_tag="VM841_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaconate CoA-transferase"
                     /protein_id="HVL88894.1"
                     /translation="IDGRMLMGSGGANDVATAATCSIVTAAQERDRFVDKVEYITSPG
                     DRVVAMATTHGIYRKDQGEFVLTAVHDEDLEAGVKAAVAKCGWDLRVAPTVEKIPPPT
                     PSEIRTLRLLDPNGYFRRR"
BASE COUNT          794 a         1971 c         1974 g          818 t
ORIGIN      
        1 cgaactagcc ggcagcgagc gttcccgcag gcgaggcaac gacgagcggc gtccaagtcg
       61 aaccggcgga cccaccgaga ccaccaagcg ctgcgggccg tcgatctgga tccggccggt
      121 tgcccagaag caccacgagg gacgttctgg caggaatgct gcgcgagcgg gcaggcggcg
      181 acggcgaggc cgcgctcacc aatcccatcc gtatcgatcg gaatcaaata gtccgcccga
      241 tctttcgcat cggagcctcc tgggtagact ggaacggacg gcgagactca ctcccagggg
      301 gctccttgga ttggttcgtt gacactacgc ggcgtgaagc cgtggaagaa gtcaaggccg
      361 agatcggccg gtacgtgcgc cgccacgccg acgatcccgg ccggctgttc gacgccgacg
      421 tcgtcgtctc cgagctgctg agcaacgcgg tccggcacgc cgccggccct gcctgggtct
      481 cgatcgtgtg gaccaccccc cgcccggttc tgagcgtcta cgatctgggt cccggattcg
      541 tgttcgatcc caaactgccc gacgatccgc tggccgaagg cggccgcggt ctctacatcg
      601 tcagccacat ggtggacgag ctcgacgccg cctcgcggcg cgcaggcggc acgcgcgtcg
      661 aagcggtcct gccggtcacg cggcaaagct ccgacaccta cgatccgccg cgccggcgca
      721 gcggcgcgtt gccccatccg gacgaggcgc agcccggcgg cggattcggc aaggaatcct
      781 tcctgcgcgc gctggtcgtg cagctggcgc aggcgatcga agccgatcac ggaccggccg
      841 cggccgaagc ggccgtcgcg caggtggccg ccgacgtcgg cggccggatg gaagacgagt
      901 accgcgccgc gaaagacatc gccgggcgca tgacacccgg ccagatggcc gactgctacg
      961 tccgtctcaa gcacgcgatc gacggcggct tctacgtcat agaagcaacc gaagggcgca
     1021 tcgtgctcgg caacaccgcc tgcccgttcg gatccgtggt gacccgcgcg ccggcgctgt
     1081 gccgcatgac gtcatcggtg ttcggcggca tcgccgcgcg caacagcgaa aaggaagcat
     1141 ccgtcgtgct ggaagagcgc atcgccgtcg gcgatcccgg ctgccgcgtc gtggtgtacc
     1201 tggaccgccc ccccgacgac cccggcatcg cgcatcacta cgcgacaccc gttgatgagc
     1261 tcgagcccga gcccgaagcg acgcggtagc gtccgctcag ctccagaacg ccgcgcccag
     1321 caagccggga ccggtatgcg cgcccatcag cggcgtgaac tcggccaggt gcgcctcggc
     1381 gggattcagc tcggcggtca atcgatccag cagcgcggcg gcttcgtcgg gcgcagccgc
     1441 atggcacacg gccagatgca gcgggccctg cgcggccgcg gtgcgcgcct cgtcggcgac
     1501 cttgtccacg gccttcccgc gcgaacgcgg ccggccgagc tgctcgactt ttccgccgcg
     1561 gaacgcgaag acgggcttga tgcccagcgc ggttcccgcg taggccatca gcgcgtggac
     1621 gcggccgctt cgcttcagat attcgaacgt gttgatcgtc gccacgacct gcgtgcggga
     1681 cgagacgtcg cgggcgcgcg cagcgaccgc atcggcatcc gcgccagacc gcgcggcacg
     1741 ggcggcttcc agcgcgatga aaccctcccc catcgacgcg ctggccgaat ccaccacgat
     1801 cacgcggccg ccgaagtcgc gcgcggcgat cgtggccgat gtatgggtga cgctcacgaa
     1861 cgaggcgacg gtgacgcaca cgaccgcatc ggctttcttg agcgccgcgg caaacgcatc
     1921 gtggaagtcg ccgacgctcg cgcccgcggt ggagacgggc acggtttcgg tttgcagccg
     1981 ctcgtagaag tagcccggcg gaatgtccac cccgtcgcgg taggtctcgt cgccgaactt
     2041 caaatacatc ggcacgatgt cgatgtcgtg ttctgcggcg gcggcggccg ggatggtcgc
     2101 ggccgagtcg gtgacgatcc ggacggtcac gagtcgcgca ggtcggagac gctcatcatc
     2161 gtctcctcgt accgcgcccg caatcccgtc cacgattgat acagcccgtc gtagagcgcg
     2221 agccagttcg gatcgggacg caccagctcg ccctgatcgg acatcttcgc cgcggccgcg
     2281 gcgacggtcg gatgcagccc cgccgcggtc gcggcgacga tcgcggcgcc cagcgcgctg
     2341 gcatgcggct cgcgcgcgcg ccgcacctcg cggcccagca ctcccgcgag cacccgcacg
     2401 aacgtggggc tgcgcgacag gccgccgcag acgctgacca ggccggcatc gcggcccgag
     2461 acctcttgca gccattcgat gccggcgcgc gccgcgtacg caacgccttc cagcaccgcg
     2521 cgggacagat cttcgcgtcc ggggttgagc gccgagaccg ggatcggaaa cgccaggccg
     2581 ccgctgcgca tcaccggata gtcgccgacg ttgatcggac ccggatccac gatcgccacg
     2641 ccgcggctgc cggggtcgct ctcgccggcc gactgctcga gccacagatg gttgccggcc
     2701 tcgccgatca agccgagcag ccagtccacc agcgcacccg cttcgccgca gttgacttcc
     2761 aggatcgagc gtccgggcac aacgtgcggc gaggtcacca gacggcgctg cgcgtcgatg
     2821 atcgcctcgc cttggacctg ggtgaccggc atcatcgtgc cggcgttgat cgcgacgtcg
     2881 ccgggctcga tcacgcccat cgccagcgcc gcgcattgcg tgtccgcgcc cgcgcgcacg
     2941 acgggcgtgc cggggtgcag gccgaactcg cgcgccacgt cttcgggcag ccggcccagc
     3001 ggctcggtca tcacgcgcgg caagaaccgt gacggaacgt ccagcgcgtc gcagagcgtc
     3061 tgcgaccaca tctcgcgccg cacgtcgaag cacatcgtct cggcggccat cgtcggatcg
     3121 gtcgcgatct cgccggtcag gcgaaacgcg atccagtcgc cgaacgacag cgcgtgcgcg
     3181 gttcggttgg cgatctcggg cttgttgccg cggaaccaag cgagccgcgc gggcagatat
     3241 ttgaaggcgg gcagccggcc ggtggtctcg tagatcaggt cgccgtgctc gcgctccagg
     3301 gcgatgcctt cgttggcggc gcgcccgtcg ctgtcgggtc cggcgtagag gatctggcct
     3361 ttggagtcca gcagcgcgac gccgttgcgc tgcgcggtga tgccgatgac ggcgatcgcc
     3421 tcgggccggg ccagcgacga gcgcacggct tcgcgggtgg cgctggccag cgaggcccag
     3481 acgctgcccg tgtccagaac gggaaaaccg tccggatccg tgcggtagcg ccagcggcgt
     3541 tccgcggatc cgatccgctt tccccccgag tcggcgatga gcgcccggcc gccggaagtc
     3601 ccgacgtcga gcgcgagaac gaggctcata gcgggcgtaa gggtaccggg tggggcacgc
     3661 gaacaggaaa acggccccca ggggtggccg gggaccgttg ggaggttcga tcggcggcta
     3721 tctgcccctg tttttcttgc cgggcgcgtc gccgctgcgg ccgggcgcgt cgggcttgcc
     3781 gcggtcttgg gtctgctggc ggcgctcctc ggtgcgctgc tgctgccgct cggcgttgtc
     3841 gatggcgcgc tccaggcctt gctgggcctg ctcgggcacc tggttcaaga tctcgttcag
     3901 gtgcgcgatg tgagcgcggg tgcgttccag gatccgcccc agcgcttcgc tcgaggcgcc
     3961 ctcgccggcg gcctcgaggg aggcggcgaa gtcggctgcc aggtcggtga cgtcttgtcc
     4021 ttcggcggcc agcgcgtcga tctcgtcgat gcgccgctct gccagctgca ggtgcagcgc
     4081 ggccttggat tcgggcgaca gccgcatcgc cagcgcgatc cgctcggagg cgcgcttgac
     4141 gccgtagagg gcatcgcccg gcttggtccc ggcggcggcg gccagcgcgc tgccgcttcc
     4201 gaaagtcagc agcatcgcgg cggccagcac cgcgaccgcc ccgcggcggc ggccacggcc
     4261 gactcgccgc ttcctcacat cgtcgatggg gacgatgtcg gcggcggggc gccgctgcgc
     4321 gtcagggtac cgctcggggg aggacagctt ggtcgtgtcg ggctcagggc tgcgttcggg
     4381 cgaggcgagc tgcgggtccg cggccgagcg gatcgcagcc agatggtccc gggcgaccgc
     4441 gtccggcacc tcgcggcgca gcgccgcgcg aatcatggaa gcctccggca gcagcgcctc
     4501 gtcgcccgcg acgaccgcgt ccagcgcacg gtccagcgcg tcggtgtcgc ggtcgtggaa
     4561 accctctcgg tcggtcatct cacccttcca agcgacgggg gacggccggc gggtacgggc
     4621 tcggaccgga tgtcggcggt atttgcggca tttgcttgcc gagcgtcttg gcgaggttgc
     4681 cgagcgcgcg atgctgcagc gccttgatcg cgttgacgtc ctttccgacg acctcggcga
     4741 tctcgcccga agacaggccc gagccgaggc ggagcgcgat cacctcgcgt tgcgcgtcgg
     4801 gaagcgcggc gacggcctcc cagacgcgcc ggcgctccag cgcggccagg gcctgctctt
     4861 cggttccctg ctcggcgggc gcgtcgccgg cggcctcgag cggctcttcg gggcggcggc
     4921 tctggcggcg gccgaggtcg gtggcgcggt cgcggccgat gcggaacagc caggcgcgga
     4981 aggcggcggc gtcgccttcg aaccgatcga tgcctcgcat cgcttccagg aacacctggc
     5041 cgaccaggtc ctcggcatcc tcggtccgtc tcacctgtgt cgccagaaac gacagcagcg
     5101 gtcccgcgta gacgcggtag agatcctcga atccgttgcg atcgcccgcg agcacccgcc
     5161 cgagcgcggc ggccggatcg acgccgccgc tcatcggcgg cggaagtagc cgttgggatc
     5221 cagcagtcgc agcgtgcgaa tctccgacgg ggtcggcggg gggatcttct cgacggtcgg
     5281 tgcgacgcgc agatcccagc cgcacttcgc gaccgcggcc ttcacgccgg cttcgaggtc
     5341 ctcgtcgtgc acggcggtca ggacgaactc gccttggtcc ttgcggtaga tgccgtgcgt
     5401 ggtcgccatc gcgacgacgc gatcgcccgg cgaggtgatg tattcgactt tgtccacgaa
     5461 ccggtcgcgt tcctgcgcgg cggtcacgat gctgcacgtc gccgcggtcg cgacgtcgtt
     5521 cgcgccgccc gagcccatca gcatgcggcc gtcgatg
//
LOCUS       DATLFO010000046        11315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_91482, whole genome shotgun sequence.
ACCESSION   DATLFO010000046 DATLFO010000000
VERSION     DATLFO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91482"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..378)
                     /locus_tag="VM841_01540"
     CDS             complement(10..378)
                     /locus_tag="VM841_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVL88895.1"
                     /translation="MEETAYGLFQRGAALLSEGMVREAAESLERAREMEPDKASIREA
                     LARAYYAQGRTTDARAEFSAAACIDPSNDYAHFGLALCLERENRLDEARGHARLAVAM
                     KPENEDYRRALDRIERSGAS"
     gene            511..1749
                     /locus_tag="VM841_01545"
     CDS             511..1749
                     /locus_tag="VM841_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HVL88896.1"
                     /translation="MPDVAAFQGFRYAPGVEPAAVTSPPYDVISPRERAALEAASPHN
                     VVRLILGPLGVSDDRKYIEARRLLDAWIDEGVLVRDDAPSLYLYEERFEVDGERRSQV
                     GLLAAVEISDEVMPHERVMGPVVDDRLAVIRTTEANLSPVFCLYWSRDDAARRVIEAT
                     MAEEPYAAFTDEGGVDHRAWRISDQERIKAVAAALAGAKTVIADGHHRYRTAEAYKEE
                     RRADDGPGPWDRMLLYLVDAERDGPALLPIHRVVHDLAVEDALDKLSEHFEVEEVPAA
                     DPDELALLVNRRRAEGRSYALLGPKSSWIVTLRDEKVAAQATEGDHSDAWRDLDVAVL
                     HSLVFEQILGGAAATYVHHPAEAEEAVQTGEATFAVLLARTPTAAVREIAEAKDSMPP
                     KSTFFIPKPRTGIVIRSLRV"
     gene            1975..3468
                     /locus_tag="VM841_01550"
     CDS             1975..3468
                     /locus_tag="VM841_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL88897.1"
                     /translation="MRRAGRIDRRIVLSVVLGLIGTACTIQGPRIVLEGQGGGEGVPG
                     VVQTGAGDPGQGTVVGGTKVGGGSVGGGGGGGGPIVADDSCKGKATDVGVTGNSIKLG
                     STFAISGPVSNISGPILKGVQAYLNEINATGGVHGRKVEFKFYDDGWDAQRGKSKIKQ
                     LVEQDKVFMLVSVPSSNGLDAATGYLDQKKVPVLGTSGLIETQFKAHMQWPVGTSTRS
                     AARIGLIDATQRLQAKTFAIVWLDLLAGYEAREAIFEAAKRGFQGLNEKSIVADRRIS
                     ISEPSFESVWNDVQNQARKNGVADGRPDQIVLAIDPTNAIKAMQAAERLGFRPKKGWG
                     GAAPLFLDLVLRDAPYAVKTGMTAGTAFFPPVGQFANQPAVKKYVQVVRKYFADVDLN
                     NPYLQGGYTGAALAIDVLEKVGSCVTRERVIQAANSLTNHAPAGLTNPLTFRPLGEGP
                     GHYGNTYGLVVQAVPKGTAGCTRSIGCWNVIPASSSDPFYKDPTPGR"
     gene            3481..4392
                     /locus_tag="VM841_01555"
     CDS             3481..4392
                     /locus_tag="VM841_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL88898.1"
                     /translation="MKFYIQNIILGLPIGVIWALLATSVVMIYRASRVLNLAVGGISM
                     ISAYMLFQMANDERWGIPLPIAVVLTILFAAAIGWIIERILLRPLRDRPTLVSVIMTI
                     GILSLTTEVASMVWTVDRQPAPNLLPTGAIRFWGIALGVDQILTFFITMAIVGVVIYL
                     FRATTLGVAMRAVSDDRRAALLMGVPVDRVSSLTWIIGSVLGAIAGILVAPQVGLTPL
                     GMVFQSIPALAAALFGGLVSIELAFGGAMVLGVLWSVLPGVELGAWRLHDHVIGVQQI
                     AIFALVVVFLVVRWQQLFGSAAKEEEI"
     gene            4392..6335
                     /locus_tag="VM841_01560"
     CDS             4392..6335
                     /locus_tag="VM841_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVL88899.1"
                     /translation="MAIAVRAPAEKRSLLVRVPRLRTVGLLFLVIWPWVLPAQPWSSM
                     AVAAAIQSLALVSLVVLTGWVGQISLAQAAIMGVGAFSAAQITMHLGIDFPFHVPIAG
                     LASAGTAVLIGLPALRIKGLYLAIATLAFQWMLEESLLQMHWFSGGFNGVTIPTPKIA
                     TYSLSNERLFYYFAWGIAALLMLLVANMRDSKTGRAWFAIRGSEIAARSMGINVTRYK
                     LLAFAVSGFVIGVAGSLKLNTVGSATPLQYLFPFSITFLALAVLGGIGSLAGAVAIAS
                     IFIFLQDYGFPQIVPSLAQKGALPILAAALLIVQLITNPGGLAATKEKMKEDIAKRHL
                     RKKMRQARKNPGPAASQAAALHESATTITLADPEAAARLAERVAARGERAHRLDSPAL
                     LRAEDVTIRFGGVVANDKVSLEVRRGEITGLIGPNGAGKTTHFNTLNGLLVPDEGRIH
                     FAGKDITDMPTHERAAMGMSRTFQIMRLFPRLTVFENIMVGTHLQNTAGVTSNVFMLP
                     RSRNEDARLSERCREILALLGIDHLADERVAGMPFGILRLVELARALVASPQLLLLDE
                     PASGLDVSETDAFSEHLMRIRDEMGPTILLIEHDMRLVMNVSDYVYVLEFGKNLAEGV
                     PSEIQQNPDVIAAYLGGEGVTVG"
     gene            6328..7413
                     /locus_tag="VM841_01565"
     CDS             6328..7413
                     /locus_tag="VM841_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL88900.1"
                     /translation="MASTARDLAADPILRVEGIDVYYGHVQALRGVSLEIYPGELVAL
                     VGANGAGKTTTLRAISGLLKPVVGHITLDGQRIDTLPPEKVVGLGVSHLPEGRGIFPT
                     LTVFENLQMGFYTKRSDRKAFNHGVEQMVELFPRLGERMQQAAGTMSGGEQQMLALAR
                     ALLPGPRLLMIDELSLGLAPVIVQMLFERLKEINEAGTTVLLVEQYVNLALQVADRAY
                     VLEKGGVALSGTAEDLARDSEIVRSSYLGGHAPAPAAEPASATEPARAAEPLPAPSVV
                     TDRDEPEQRFLRAVESERIPATKVTSGPRKKSPAKKTAAKTKAAAEKAPAKKKAPAKK
                     KAPAKKTAASGTRSAAAKKSATTTKEG"
     gene            7423..9213
                     /locus_tag="VM841_01570"
     CDS             7423..9213
                     /locus_tag="VM841_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88901.1"
                     /translation="MGVMTRRTTEQRRRLWRWVALAMLGLFTWSFIASPNTAASNAAG
                     DEEITEPALAAYHTLADASPIRGLIDHQTYLVSAEPAVGRSTSEVALPSQASATAWLT
                     TFGTVDGLHGTTTGNKVPTETTATQPGGDPEQEFALHRGSLGNDELFQFGAGVSRAKA
                     VRSNRPRGFGHAYLANLALLPAPGSPEAPPGSYDPEADKAKEKENPPSPGADDPQAYT
                     PNPKAQMAILSVGSVASTAETFREESKVVSIAVAELNGISIGNRTADGRCTNCITIDS
                     MRVEARTESDGTKDGALASWRILVHRACRVAFTNDPQTGAAYEGVQCLNPNPDGFVAA
                     IEGSSADQGREALQDPNARGVRQVEQAEKLNELFASLEKALGVKDLGVTLRVGTDRDN
                     AAHVDPSDYSAAAVARGLVLEFRTAVTSQVLDEIAGNQSLQGLIQVVDGGCGAVAGPA
                     GQHLPPLPPPASAIGVPAACGTGAIQSGQTMRTLRIAFGQVRTASKAVPGDADFGGGD
                     DDDTGIPPFPEMPVIDIPEIEIPEFNIPPGGGNTTVIAGLQGGPLKMRIDWGSMGIKP
                     WKPRDMAKGFLSGGMALGLWALVRRRLSLL"
     gene            9379..10329
                     /locus_tag="VM841_01575"
     CDS             9379..10329
                     /locus_tag="VM841_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL88902.1"
                     /translation="MTPPPIVRYPDGIIAIDCEMAGHHGLIAAFVIEGPNPVVIETGP
                     ATVMPDLTAGLDSLGIGRTDVATFVGTHIHLDHAGAGGDVADAYPSATVAVHEWGVKH
                     MADPSKLMASAYRVFGDRLDTLFGPLKPVPQDRLRALDEGDVIDAGGGRQIRVLYTPG
                     HARHHITLHDTQTDAIFVGDSMGVYLPEAGILRPATPPPDFDFHLALSALERYRELDP
                     ATIYFSHFGPAPAGRDMITEARRKLIEYARIVREAMRQSEDLDHVSEKLVEATASEYA
                     EVYADPELSARFDALNAFRSSAAGYLRYFQQNPQAEIPGV"
BASE COUNT         1789 a         3913 c         3822 g         1791 t
ORIGIN      
        1 gcgcgaggat cacgacgccc cgctccgctc gatccggtcc aaggcgcgcc ggtagtcctc
       61 gttctcgggc ttcatcgcga ccgcgagcct ggcgtggccg cgcgcctcgt cgaggcggtt
      121 ctcccgctcg aggcacagcg ccaggccgaa gtgggcgtag tcgttactcg ggtcgatgca
      181 ggccgcggcg ctgaactcgg cgcgcgcgtc ggtggtgcgg ccctgcgcgt aataggcccg
      241 cgccaaggcc tcgcggatcg aagccttgtc gggctccatc tcgcgggccc gttcgagcga
      301 ttcggcggcc tcgcggacca ttccttcgga caggagggcc gcgccgcgct gaaacagccc
      361 gtacgcggtc tcttccattc gtcctcgctc cttcgttcgt tggttcgttc gttggttcgg
      421 gcgttggttc gggcgttggt tcgggcgttc gttcgggcgt tcgttcgggc ggttcgcccg
      481 ggtgccccgc aggatgctat acacccggcg atgcccgacg tcgctgcttt ccaaggcttt
      541 cggtacgcgc ccggcgtgga gccggccgcc gtcacgtccc caccctacga cgtgatctcc
      601 ccgcgcgagc gggccgcgct cgaggcggcc agcccgcaca acgtcgtccg cttgatcctc
      661 ggtccgctgg gcgtatcgga cgaccggaag tacatcgagg cccgccggct gctggatgcg
      721 tggatcgacg agggagtcct ggtccgcgac gacgcgccct cgctctacct ctacgaggag
      781 cgcttcgagg tcgacggcga gcggcgaagc caggtcggcc tgctggccgc ggtcgagatc
      841 tccgacgaag tcatgcccca cgagcgggtg atgggcccgg tggtcgacga tcgcctggcc
      901 gtgatccgaa cgaccgaggc gaacctgtcc cccgtctttt gcttgtactg gtcccgggac
      961 gacgctgccc gccgggtcat cgaagccacc atggctgagg agccctatgc ggcgttcacc
     1021 gacgaaggcg gcgttgacca cagagcgtgg cggatcagcg accaagagcg catcaaggcg
     1081 gtcgcggccg ctttggcggg cgccaagacc gtgatcgccg acggccacca ccgctaccgc
     1141 accgccgagg cctataagga ggagcgcaga gccgacgacg gccccggccc gtgggaccgg
     1201 atgctgctct acctcgtgga cgccgagagg gacggcccgg cgctgctgcc gatccaccgg
     1261 gtcgtccatg acctggccgt cgaggatgcg ctggacaagc tgtccgagca tttcgaggtt
     1321 gaggaggtcc cggccgccga tcccgacgaa ctcgcactgc tggtgaatcg acgccgcgcc
     1381 gagggccgct cctatgccct gcttggcccg aaatcgtcct ggatcgtgac acttcgtgac
     1441 gaaaaggtcg ccgctcaggc aacggaaggc gaccatagcg acgcgtggcg agacctcgac
     1501 gtcgcggtcc tgcactcgct cgtgttcgag caaatcctcg ggggcgcggc tgcgacatac
     1561 gtgcaccacc cggcggaggc cgaggaggcc gtccagaccg gcgaggccac ctttgccgtg
     1621 ctgctcgccc gcacccccac agcagcggtc cgcgagatcg ccgaggcgaa ggactcgatg
     1681 cctcccaaat ccacgttctt catccccaag ccccgaaccg gaatcgtcat ccggtccctt
     1741 cgcgtatagt tcgcgatttt tccgggaaga ttcccttgtt gcggtgctta gtccatgcca
     1801 ggatcgatag gcaagcgaac acaccgagtg gccaccctta aaggattcga tgccgcttcc
     1861 atagaatttg accccgacga gttgggtgat tcgcgccagc gaacctcgtc cggccgccgc
     1921 cgatcccccc gaagatcgcg gcggggccgg aagtggcgag agggaggcaa cgagatgagg
     1981 cgtgccggac gcatcgaccg caggatcgtg ctgagcgtcg tcctggggtt gatcggaacc
     2041 gcgtgcacga tccagggacc gcgcatcgtc ctggaaggtc aagggggcgg cgaaggtgtt
     2101 cccggcgtcg tccagaccgg ggccggcgat cccggtcagg gcaccgtggt cggcggcacc
     2161 aaggtcggcg gcggatccgt cggcggcgga ggcggcggag gcggcccgat cgtggccgac
     2221 gactcctgca agggcaaggc caccgacgtc ggcgtgaccg gcaactcgat caagctcgga
     2281 tccactttcg cgatctccgg cccggtctcc aacatctcgg gcccgatcct caagggcgtg
     2341 caggcctacc tcaacgagat caacgccacc ggcggcgtgc acggccgcaa ggtcgagttc
     2401 aagttctacg acgacgggtg ggacgcccag cgcggcaagt ccaagatcaa gcagctcgtc
     2461 gagcaggaca aggtcttcat gctggtgtcg gtcccgtcct cgaacggcct ggacgcggcc
     2521 accggctacc tcgatcagaa gaaggtcccg gtgctgggaa cgtccgggct gatcgagacc
     2581 cagttcaagg cgcacatgca gtggccggtc gggacctcga cccgcagcgc cgcgcgcatc
     2641 ggcctgatcg acgcgaccca gcggcttcag gccaagacct tcgccatcgt gtggctggac
     2701 ctgctggccg gatacgaggc gcgtgaagcg atcttcgagg ccgccaagcg aggcttccag
     2761 ggcctcaacg aaaagtcgat cgtcgccgac cgccggatca gcatctcgga gccgtcgttc
     2821 gagtccgtct ggaacgacgt tcagaaccag gcccgcaaga acggcgtcgc cgacgggcgt
     2881 cccgaccaga tcgtgctggc gatcgacccg acgaacgcga tcaaggcgat gcaggcggcc
     2941 gagcgtctcg gcttccgccc caagaagggc tggggcggcg ccgctcccct gttcttggac
     3001 ctggtgctgc gcgacgcgcc gtacgcggtc aagaccggca tgaccgccgg caccgcgttc
     3061 ttcccgccgg tcggccagtt cgccaaccag cccgcggtca agaagtacgt gcaggtcgtg
     3121 cgcaagtact tcgccgacgt ggacctcaac aacccgtatc tgcagggtgg ctacacgggc
     3181 gctgcgctcg cgatcgacgt gctggagaag gtcggaagct gcgtgacgcg ggagcgggtc
     3241 atccaggcgg cgaattcgct gacgaaccac gcgccggccg gtctgaccaa cccgctgacc
     3301 ttccggccgc tcggcgaggg gccgggccac tacggcaaca cctacggcct ggtggtgcag
     3361 gcggtgccca agggcacggc gggctgcacg cgctcgatcg ggtgctggaa cgtgatcccg
     3421 gcgagctcga gcgatccgtt ctacaaagac ccgacgcccg ggcgatagga aaggacccct
     3481 gtgaagttct atatccagaa catcatcctg ggccttccga tcggggtgat ctgggcgttg
     3541 ctcgcgacca gcgtcgtgat gatctaccgg gcctcgcgcg tgctcaacct ggccgtcggc
     3601 gggatcagca tgatctcggc ctacatgctg ttccagatgg ccaacgacga gcgatggggc
     3661 atcccgctcc ccatcgcggt cgtgctgacc atcctgttcg ccgccgcgat cggctggatc
     3721 atcgagcgga tcctgctgcg gccgcttcgc gaccggccca cgctggtgtc ggtcatcatg
     3781 accatcggca tcctgtcgct gaccaccgag gtcgcctcga tggtctggac cgtggaccgc
     3841 cagccggcgc cgaacctgct gccgaccggc gccatccgct tctggggcat cgcgctcggt
     3901 gtcgaccaga tcctgacctt cttcatcacg atggcaatcg tcggcgtggt catctacctg
     3961 ttccgcgcga cgacgctggg cgtcgcgatg cgcgcggtca gcgacgaccg ccgcgccgcc
     4021 ctgctgatgg gcgtgcccgt ggaccgggtc tccagcctca cctggatcat cggctccgtg
     4081 ctgggcgcga tcgccggcat cctggtcgcg ccccaggtcg ggctcacgcc gctcggcatg
     4141 gtgtttcagt cgatccccgc gctggcggcg gcactcttcg gcggcctggt ctcgatcgag
     4201 ctcgccttcg gcggcgcgat ggtgctgggc gtgctgtggt cggtgctgcc cggtgtcgag
     4261 ctcggcgcct ggcggctgca cgatcacgtc atcggcgtgc agcagatcgc gatcttcgcg
     4321 ctcgtggtcg tcttcctggt cgtgcgctgg cagcagctgt tcggctcggc cgccaaggaa
     4381 gaggagatct gatggcgatc gccgtccgcg ctcccgccga gaagcgctcg ctgctggtcc
     4441 gcgtgccccg cctgcgcacg gtcggcctgc tgttcttggt gatctggccg tgggttctgc
     4501 cggcgcagcc gtggtcgtcg atggcggtcg cggccgcgat ccagtcgctc gcgctggtgt
     4561 cgctggtcgt tttgaccggc tgggtcggac agatttcgct cgcccaggcg gcgatcatgg
     4621 gcgtgggcgc gttcagcgca gcgcagatca ccatgcatct cggcatcgac tttccgttcc
     4681 acgttccgat cgccgggctc gcctcggccg ggaccgcggt gctgatcggc cttcccgcgc
     4741 tgcggatcaa gggcctgtac ctggccatcg cgacgctcgc gttccaatgg atgctcgaag
     4801 agagccttct gcagatgcat tggttctcgg gcggtttcaa cggcgtgacc atccccacgc
     4861 ccaagatcgc gacctactcg ctgtcgaacg agcggctctt ctactacttc gcctggggca
     4921 tcgcggcgct gctgatgctg ctggtcgcca acatgcgcga ctccaagacc ggacgcgcat
     4981 ggtttgcgat ccggggcagc gagatcgcgg cccgctcgat gggcatcaac gtgacccgct
     5041 acaagctgct cgccttcgcg gtcagcggct tcgtgatcgg cgtcgccggc tcgctcaagc
     5101 tcaacaccgt cggctcggcc acgccgctgc agtacctgtt cccgttctcg atcacgttcc
     5161 ttgcgctggc ggtcctgggc ggcatcggct cgctggccgg tgcggtcgcg atcgcatcga
     5221 tcttcatctt cctgcaggac tacggcttcc cgcagatcgt gccctcgctg gcgcagaagg
     5281 gcgcgctgcc gatcctggcg gccgcgctgc tgatcgtgca gctgatcacg aacccgggtg
     5341 gtctggccgc gacgaaagag aagatgaaag aggacatagc caagcggcat ctgcgcaaga
     5401 agatgcgcca ggcccgcaag aatcccgggc cggcggcgtc gcaggcggcg gcgctccacg
     5461 agtccgcgac caccatcacg ctggccgatc ccgaggcggc cgcgcggctt gccgaacgcg
     5521 tcgccgcgcg tggcgagcga gcgcaccgtc tggactcgcc cgcgttgctg cgcgccgagg
     5581 acgttacgat ccgcttcggc ggcgtggtgg ccaacgacaa ggtcagcctg gaggttcgcc
     5641 gcggcgagat caccggtttg atcgggccga acggcgccgg caagaccacg catttcaaca
     5701 cgctcaacgg tctgctggtc cccgacgaag gccgcatcca tttcgccggc aaggacatca
     5761 cggacatgcc gacgcatgaa cgcgccgcga tgggcatgtc gcgcaccttc cagatcatgc
     5821 gcctgttccc gcgactcacc gtgttcgaaa acatcatggt cggcacgcat ctgcagaaca
     5881 ccgcgggcgt cacgtcgaac gtgttcatgc tgccgcgcag ccgcaacgaa gacgcgcggc
     5941 tgagcgaacg ctgccgcgag atcctcgcac tgctgggcat cgaccatctg gccgacgaac
     6001 gggtcgccgg tatgccgttc ggcatcctgc ggctggtcga gctggcccgg gccttggtcg
     6061 cctcgccgca gctgctgctg ctggatgagc ccgcatcggg cttggatgtc agcgaaaccg
     6121 acgcgttcag cgagcatctg atgcggatcc gcgacgagat gggtcccacg atcctgttga
     6181 tcgagcacga catgcggctg gttatgaacg tctcggacta cgtctacgtg ctggagttcg
     6241 gcaagaacct ggcggaaggc gtgccgagcg agattcagca gaacccggac gtgatcgccg
     6301 cgtacctggg cggagagggg gtcaccgttg gctagcaccg cgcgcgacct ggccgccgat
     6361 ccgattcttc gcgtcgaagg cattgacgtc tactacgggc acgtgcaggc gctccgcggc
     6421 gtctcgctcg agatctaccc gggcgagctc gtcgcgctcg tcggcgcgaa cggcgccggc
     6481 aagaccacga cgctgcgtgc catctcaggc ctgctcaagc ccgttgtcgg tcatatcacg
     6541 ttggacggcc agcgcatcga cacgctgccg cccgagaagg tcgtcggcct cggggtgtcg
     6601 cacctgcccg aaggccgcgg catcttcccg acgctgaccg tcttcgagaa cctgcagatg
     6661 ggcttctaca ccaagcgcag cgaccgcaag gcgttcaacc acggcgttga gcagatggtc
     6721 gagctgttcc cgcgcctcgg tgagcgcatg cagcaggcgg ccgggacgat gtcgggcggc
     6781 gagcagcaga tgctcgcgct ggctcgtgcg ctgctgccgg gaccgcgtct gctgatgatc
     6841 gacgagctat cgctgggcct ggcgcccgtg atcgtgcaga tgctgttcga gcggctcaag
     6901 gagatcaacg aggccggcac gaccgtgctg ctggtcgagc agtacgtcaa tctcgccttg
     6961 caggtggccg accgcgcgta cgtgctcgaa aagggcgggg tcgcgctgtc gggcaccgcc
     7021 gaggacctcg cccgcgacag cgagatcgtt cgctcctcgt acctgggcgg tcacgcgccc
     7081 gcgccggccg ccgaacccgc gtccgccacg gaacctgcac gcgccgcgga gcccctgccc
     7141 gcgccttccg tggtgacgga tcgggacgaa cccgaacagc gcttcctgcg cgcggtggaa
     7201 tccgagcgca taccggccac gaaggtgacg agcgggccca ggaagaagag tccggccaag
     7261 aagaccgctg ccaagacgaa ggccgcggcc gagaaggcgc ccgccaaaaa gaaggcgccg
     7321 gccaaaaaga aggcccccgc taagaagacg gcggcgtcag ggacgcgaag cgccgcagcg
     7381 aagaagtccg cgacgacgac gaaggaagga taggagcgcg ggatgggcgt gatgacgagg
     7441 cgcaccacgg agcagcgccg ccgcttgtgg cgctgggtcg cgctcgcgat gctcggcctg
     7501 ttcacgtggt cgttcatcgc gtcgccgaac accgcggcat ccaacgccgc cggcgacgag
     7561 gagatcaccg agcccgcgct cgccgcctat cacacgctcg ccgatgcctc gccgattcgc
     7621 ggcctcatcg accaccagac ctatctcgtg tcggccgagc cggccgtggg gcgctcgacc
     7681 agcgaggtcg cgctgccgtc gcaggcgtcg gccactgcgt ggctgaccac cttcgggacc
     7741 gttgacgggc tgcacggcac caccaccggc aacaaggttc cgaccgagac gacggccacc
     7801 cagcccggcg gcgaccccga gcaagagttc gcgcttcacc gcggatcgct cggcaacgac
     7861 gagctgttcc agttcggcgc cggcgtctcg cgcgccaagg cggtgcgctc caaccgcccg
     7921 cgcggattcg gccatgccta tctcgcgaac ctcgcgctgc ttcccgcgcc cggcagcccg
     7981 gaagctccgc ccggcagcta cgaccccgaa gccgacaaag ccaaagagaa ggagaacccg
     8041 ccgagccccg gcgccgacga cccgcaggcc tacacgccca acccgaaggc gcagatggcg
     8101 atcctgtcgg tgggcagcgt cgcgtccacc gccgaaacgt tccgggaaga gtccaaggtc
     8161 gtgtcgatcg cggtcgccga gctcaatggg atcagcatcg gcaaccgtac cgccgacggc
     8221 cgctgcacca actgcatcac catcgactcg atgcgtgtcg aggcccgcac cgagtcggac
     8281 ggcaccaagg acggcgcgct cgcctcgtgg aggatcctgg ttcaccgcgc atgccgtgtc
     8341 gccttcacca acgacccgca gaccggcgcc gcctacgagg gcgtgcagtg cctgaacccg
     8401 aacccggacg gcttcgtggc ggcgatcgaa ggctcgtcgg ccgaccaggg ccgcgaggcg
     8461 ctgcaggatc cgaacgcgcg tggtgtgcgc caggtcgaac aggcggagaa gctcaacgag
     8521 ctgttcgcct cgctcgagaa ggcgctcggc gtcaaggacc tcggcgtgac gttgcgcgtc
     8581 ggcaccgacc gcgacaacgc cgcgcacgtg gatccctccg actactcggc cgccgccgtc
     8641 gcacgcggcc tcgtgctgga gttccggacg gcggtcacct cgcaggtgct cgacgagatc
     8701 gccggcaacc agtcgctgca aggcctgatc caggtcgtgg acggcggctg cggtgccgtc
     8761 gcaggccccg cgggtcagca cctgccgccg ctcccgccgc ccgccagcgc gatcggcgtg
     8821 cccgcggcgt gcgggaccgg cgcgattcag tccggccaga ccatgcgcac cctgcgcatc
     8881 gccttcggcc aggttcgcac cgccagcaag gccgttccgg gcgacgccga cttcggcggt
     8941 ggcgacgacg acgacacggg catcccgccg ttccccgaga tgcccgtcat cgacatcccc
     9001 gagatcgaga tccccgagtt caacatcccg ccgggtggcg gcaacaccac cgtgatcgcc
     9061 ggcctgcagg gcgggccgct caagatgcgg atcgactggg ggtcgatggg gatcaagccg
     9121 tggaagccca gggacatggc caagggattc ctcagcggcg gcatggcgct cggcctgtgg
     9181 gccctggtcc gcaggcgcct gtcccttctc tagccgcgag ggggacggat tcgacgaacg
     9241 cagcgcatcg gactcgccga tgccgtcatc gccgccaccg cgatcctgcc ggaccgcagg
     9301 ctcgtgacgc gcaacgagat ttccgcggca ccagaaggtt tcgccgccgc atcccgtaac
     9361 cgctgctata accacgtcat gaccccacca cctatcgtcc gctatcccga cggcatcatc
     9421 gcgatcgact gcgagatggc cggccatcac ggcctgatcg ccgcgttcgt catcgaaggg
     9481 ccgaatccgg tcgtgatcga gaccggcccg gccacggtca tgccggacct gaccgcgggc
     9541 ctggattcgc tcggcatcgg ccggaccgac gtggccacgt tcgtcggcac gcacatccac
     9601 ctcgatcatg ccggcgcagg cggcgacgtc gcggacgcct acccctcggc gacggtcgcg
     9661 gtgcacgagt ggggcgtcaa gcacatggcc gatccctcca agctgatggc cagcgcctac
     9721 cgggtgttcg gcgaccggct cgacacgctg ttcggcccgc tcaagccggt gccgcaggat
     9781 cgcctgcgtg cgctggatga aggcgacgtg atcgacgcgg gcggtggccg gcagatccgc
     9841 gtgctgtata cgcccggtca cgcgcggcac cacatcacgc ttcacgacac gcagaccgac
     9901 gcgatcttcg tcggggactc gatgggcgtg tacctgcccg aggcggggat cttgcggccc
     9961 gccactcccc cgccggactt cgacttccat ctggcgctgt cggccttgga acgctaccgc
    10021 gagctggatc cggccacgat ctacttcagc catttcggcc ccgcgccggc cggccgcgac
    10081 atgatcaccg aagcccggcg caagctgatc gagtatgccc ggatcgtgcg cgaggcgatg
    10141 cggcagagcg aagatctcga ccacgtcagc gaaaagctgg tcgaggcgac cgcgtccgag
    10201 tacgccgagg tctacgccga tcccgagctc agcgctcggt tcgacgctct gaacgcgttc
    10261 cggtcttccg ccgccgggta tctgcggtac ttccaacaga acccacaggc agaaatcccg
    10321 ggggtctgat ctcctggccg gcgagatccg cgaggttgag cgctgcctgc gctccggccc
    10381 ggtcgccggt gtcggccatc gctcccgccc ggacgagcgc gatcttcatt cgctcgctcg
    10441 gggtcgccgg gctggccgat tcgaggaact cgatcgcccg aaccggcctg ccgaccgctc
    10501 ggtagcaatc ggcgatctgc gggtcgtgga tcgttgaccc ggtcatccgc cggaaggcct
    10561 gcagctcagc gagggcgtcc ttgaaccgtc ccgcagcgta gagcgagacg ccgtaggcct
    10621 cgcgaaccgt gggtgagcgt ggcgcggcct ccttggcccg tgcggcctcg gtcacggccg
    10681 acgagatgtc ccccgcgaga atggcttcga tcgccttcga aagatagaac ttcgcatcca
    10741 gcgtccgtcc cgcagtgtgg tccagctcgg cgcccactcc gggcgggaga ggaaatcgtg
    10801 gcgcgtcccc cgcctttcgt tcctcggcac gggcaggtcg cagggggccg gatcccggcc
    10861 ggcggtcgct cgggcgcttc ccgcggtcgt cgctcacgaa acgcaagtat gccgttcctt
    10921 cggctttgcc gctccgggtg ggccttgacg aatctgccgg attcgggggg aatgtccggc
    10981 cgcttggttc cctcatgaca gtttcagatc cctagcggaa ggatccgggc aggtccgcgt
    11041 cgtacctacc tagcaaggag accggccgca aaagggtttg gccgtcacgg gatgaggcgc
    11101 cgccgtcgtc cggcgagcgg tgataagggg gtggctgcaa ggggcaccgg tttacgttcc
    11161 cacgtgggac ggggaacgcg tgtcgggccg aaaggctcag gcttgcagcg cttcgagagg
    11221 aggatctaga tggacctgag aaagatcgtg gccacaggga tcgcggtcgg ggcagtcgcc
    11281 gcggccatcg ccgtgccggc cgccgcgcac aacgg
//
LOCUS       DATLFO010000047         4047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_139694, whole genome shotgun sequence.
ACCESSION   DATLFO010000047 DATLFO010000000
VERSION     DATLFO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139694"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VM841_01580"
     CDS             complement(<1..662)
                     /locus_tag="VM841_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL88903.1"
                     /translation="MSALGPLGIEPTGSEGITPEELALAARNRGMPLEALRYDVTPAG
                     MHYLLTHYDIPHVDEAEWRLRIGGAVESPMSLALHELQAMPSKTLTVTMECAGNGRAL
                     LNPRPVSQPWRHEAVSTATWTGVPLRNLLEVANPLAEAVDLVFTGADHGTERGIEQDY
                     QRGLSLEDAGAPEVLLAYAMNGEPLPPQHGAPLRLLIPGWYGMASVKWLHKVEAITEK
                     FDG"
     gene            complement(684..1829)
                     /locus_tag="VM841_01585"
     CDS             complement(684..1829)
                     /locus_tag="VM841_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL88904.1"
                     /translation="MTTFRLSDEQELFRSTVRDLAERHIAPRAAEIDEKDEYPADMHE
                     LLVRNDLMAIGYPEEFGGSGGPVDFCLMIEEISRVSAGVSLIPLVNRLGAIPVMIAGN
                     DDQKKELIGGIVRGERQMAYCLTEPESGSDAGAMKSRAVRDGGGWVMNGTKRFITNAG
                     VADSYTYFAVTNPDAPKGKNVSAFIVRRDAPGFEIGREEHKMGIKGSPTREVILNNYR
                     ARGEDLLGEENLGFTYAMKTLDFSRPTIAAQALGIAQGAFDHALAYAKDRQQFGKPIV
                     QFQGIEFMLADMAMRIEASRLLVYKAAQAVVEKDPKVSYHAAIAKCYASDTAMSVTTD
                     AVQILGGYGYVKEFPVERMMRDAKITQIYEGTNQIQRVVIARALAKG"
     gene            2110..2469
                     /locus_tag="VM841_01590"
     CDS             2110..2469
                     /locus_tag="VM841_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88905.1"
                     /translation="MVLEPDTILFGTKGEDDWAFSPDVIARLDESSEGYLILEDEDRD
                     VDPADDDEGWPVLPGNQIIWIDDGDEAEPLAIVSADRAGAQEALEEVAHEWARGEDAV
                     EIEIDSSGNITAIFRDE"
     gene            2529..3962
                     /locus_tag="VM841_01595"
     CDS             2529..3962
                     /locus_tag="VM841_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009399365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVL88906.1"
                     /translation="MERAMGGLTAYGAYIPYHRLKRSEIAGALGGRAGKGTRAVASYD
                     EDTTSMAVEAARIALRGATASLERLYFATALPAYADKTNATAVHAALDLGESALAVDM
                     SGAVRSGVGALLAASESPVAAMAVLSDIRIGLPGGGDESAGGDAAAAFVFGGDAEPVA
                     DVLGFAAATQEFLDRWRVPTERVSRSWEERFGEHAYGPLADAAFADACKQAGLTPEQI
                     DHLVVAGVHPRAARAFAKSSGVRPDAVVADRSDVIGNTGTAHAGVLLADVLDRAEPGR
                     VIAVAVLADGASVLLLRTTDALAARRAAVPVQDQIDAGNDGLPYATFLSWRGFLDREP
                     PRRPDPDAPAAPPSFRMEDWKFAFVGSRCDACGAKHMPPNRVCVKCGEIDRMTRERLA
                     DVPATIATYTIDRLAYTPSPPLVMAVVDFDGGGRFRCQLTDVDPDSVAIGDRLEMTFR
                     KFVTAGGIHNYFWKARPARDAAAASSG"
BASE COUNT          574 a         1409 c         1413 g          651 t
ORIGIN      
        1 ccgtcgaact tctcggtgat cgcctcgacc ttgtgcagcc acttcacgct cgccatgccg
       61 taccagccgg gaatcagcag ccgcagcggc gcgccgtgct ggggcgggag cggctcgccg
      121 ttcatcgcgt aggccaggag cacctcgggc gcgccggcgt cctcgagcga caggccgcgc
      181 tgatagtcct gctcgatgcc gcgctccgtg ccgtggtcgg cgccggtgaa gaccagatcc
      241 acagcctcgg ccagcgggtt ggcgacctcg agcaggttgc gcagcggcac gccggtccag
      301 gtcgcggtgc tgactgcctc gtgccgccac ggctggctga ccggacgcgg gttcagcagg
      361 gcgcggccgt tgccggcgca ctccatcgtg acggtcagcg tcttggaggg catcgcctga
      421 agctcgtgca gcgcgagcga catcggggac tcgaccgcgc cgccgatgcg cagccgccat
      481 tccgcctcgt ccacgtgagg gatgtcgtag tgcgtcagca gatagtgcat gcctgccggg
      541 gtgacgtcgt agcggagcgc ctcgagcggc atgccccggt tgcgcgccgc cagcgcgagc
      601 tcctcgggcg tgatgccttc cgagccggtc ggttcgatgc ccagtggacc caacgcgctc
      661 atgacggggc tcccttcgag gcgctatccc ttggccagcg cgcgcgcgat caccacgcgc
      721 tggatctggt tcgtcccttc gtagatctgc gtgatcttgg cgtcccgcat catgcgctcg
      781 accgggaact ccttgacgta gccgtagccg ccgaggatct gcacggcgtc ggtcgtgacg
      841 ctcatcgccg tgtcggacgc gtagcatttt gcgatcgctg cgtgatacga gaccttcggg
      901 tccttttcca cgaccgcctg cgcggccttg tagacgagca ggcgcgacgc ctcgatgcgc
      961 atcgccatgt cggcgagcat gaattcgatc ccttggaact gcacgatcgg cttgccgaac
     1021 tgctgccggt ccttggcgta ggccagcgca tggtcgaacg ctccctgcgc gatcccgagc
     1081 gcctgcgccg cgatcgtggg ccgcgagaag tccaaggttt tcatcgcgta ggtgaagccg
     1141 aggttctcct cgcccagcag gtcctcgccg cgagcgcggt agttgttcag gatcacctcg
     1201 cgcgtcggcg atcccttgat ccccatcttg tgctcctcgc ggccgatctc gaagccggga
     1261 gcgtcgcggc gcacgatgaa cgccgacacg ttcttgccct tgggcgcgtc cgggttggtg
     1321 accgcgaagt acgtgtagga gtcggcgacg cccgcattgg tgatgaagcg cttcgtgccg
     1381 ttcatcaccc agccgccgcc gtcacgcacc gcacgcgact tcatcgcgcc cgcgtcgctg
     1441 cccgactccg gctcggtcag gcagtaggcc atctgccgct cgccacggac gatgccgccg
     1501 atcagctcct tcttttggtc gtcgttgccg gcgatcatca ccgggatcgc gccgagccgg
     1561 ttcaccagtg ggatcagcga cacgccggcc gacacccgtg agatctcttc gatcatcagg
     1621 cagaagtcca ccggaccgcc ggagccgccg aactcctccg gatagccgat cgccatcagg
     1681 tcgttgcgca cgagcagctc gtgcatgtcg gccgggtact cgtccttctc gtcgatctcg
     1741 gccgcgcggg gcgcgatgtg cctttcggca aggtcgcgga cggtcgagcg gaacagctct
     1801 tgctcgtcgg agaggcggaa ggtggtcatt cgtcggtctc gcaatctgat cgctgatcgg
     1861 tgggttcggc tgccgtccag cctaccggcc gctgaccgcg acacgcacga ccggcccgcc
     1921 gcgatcgggg cgacgagcgc gttcgctcgc attgcctcgc ccggaatgcc cgaggggatc
     1981 gccgtccggg acgggaattg cctccccctc gtcgagcctg ccttggagaa attctcgggc
     2041 cggagatgca atcggggcgt ggccgctgca taatccgacc gaccactctg ggatgaggag
     2101 agcgacagca tggttttgga gccggacacg atcctgttcg gtacgaaggg cgaggacgac
     2161 tgggcgttct ccccggatgt gatcgcgcgt ctggacgagt cctccgaggg ctacctgatc
     2221 ctggaggacg aagaccggga cgtggacccg gccgacgacg acgagggatg gccggttctg
     2281 cccggcaacc agatcatctg gatcgacgac ggtgacgagg ccgagccgct ggcgatcgtg
     2341 tccgcggacc gggccggcgc gcaggaggcc ttggaagagg tcgcccacga gtgggcgcgc
     2401 ggcgaagatg ccgtcgagat cgagattgac tcctcgggga acattacagc gatcttccgc
     2461 gacgagtaag cggccggtgg cgccgcccgc tccggatcgg ccaagataaa ggtccgacga
     2521 tcccgacctt ggagcgagcg atgggcggat tgaccgcgta cggcgcctac attccctacc
     2581 accggctcaa gcgcagcgag atcgcgggcg cgctcggcgg ccgggccggc aagggcacgc
     2641 gcgccgtcgc ctcctacgac gaggacacga cctcgatggc cgtcgaggcc gcgcgcatcg
     2701 cgctgcgcgg tgcgactgcg tcactcgagc ggctctactt cgccaccgcg cttcccgcct
     2761 acgccgacaa gaccaacgcg accgcggtgc acgcggcgct cgacctgggc gagtccgcgc
     2821 tcgccgtcga catgagcgga gcggttcgct cgggcgtggg cgcgctgctc gccgcgagcg
     2881 agtccccggt ggccgcgatg gcggtgctgt cggacatccg aatcgggctg cccggcggcg
     2941 gcgacgagtc cgcgggcggg gacgccgctg ccgcgttcgt cttcggcggc gacgccgagc
     3001 cggtcgccga cgtgctcggc ttcgccgcgg cgacgcagga attcctcgac cgctggcggg
     3061 tgccgaccga gcgcgtctcc cgcagctggg aggagcgctt cggcgagcac gcctacggtc
     3121 cgctcgccga cgccgcgttt gccgacgcct gcaagcaggc cgggctgacg cccgagcaga
     3181 tcgaccatct cgtcgtcgcc ggcgtgcacc cgcgcgccgc gcgcgccttc gcgaagtcca
     3241 gcggggtccg gcccgacgcc gtcgtcgcgg accgcagcga cgtgatcggc aacaccggca
     3301 cggcgcacgc gggcgtgctt ctggccgacg tgctcgaccg ggccgagccc ggccgggtga
     3361 tcgcggtcgc ggtgctcgcc gacggcgcct cggttctgct gctgcgcacc accgatgcgc
     3421 tggccgctcg ccgcgcggcg gtgccggtgc aggaccagat cgacgcgggc aacgacgggc
     3481 ttccctacgc gaccttcctg tcgtggcgcg ggttcttgga ccgggagccg ccgcggcggc
     3541 ccgatccgga cgcgccggcg gcgccgccgt cgttccggat ggaggactgg aagtttgcgt
     3601 tcgtcggctc gcgctgcgac gcctgcggcg ccaagcacat gccgcccaac cgcgtctgcg
     3661 tcaagtgcgg agagatcgac cggatgacgc gcgagcgact cgccgacgtg cccgcgacga
     3721 tcgcgaccta caccatcgac cggctcgcct acacgccgag cccgccgctg gtgatggcgg
     3781 tcgtggactt cgacggcggt ggccggttcc gttgccagct caccgacgtg gatccggact
     3841 cggtcgcgat cggggaccgg ctcgagatga ccttccgaaa gttcgtcacc gccggcggca
     3901 ttcacaacta cttctggaag gcccggcccg cgcgcgacgc ggccgccgcg tcatccggct
     3961 aggaggatca tcatggcctc gaatggaatc cgcgatcgcg tggccgtcgt cggcatgggc
     4021 tgcacgcagt tcggggaacg ctgggac
//
LOCUS       DATLFO010000048        23261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_80213, whole genome shotgun sequence.
ACCESSION   DATLFO010000048 DATLFO010000000
VERSION     DATLFO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80213"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VM841_01600"
     CDS             complement(<1..209)
                     /locus_tag="VM841_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88907.1"
                     /translation="MTPLTAAFAHAITTTLLPVVTRAVAPTDMAEMRAMLERSSSETR
                     YRRFMVPMPRIGDGPAAGCGQPSRWT"
     gene            complement(206..718)
                     /locus_tag="VM841_01605"
     CDS             complement(206..718)
                     /locus_tag="VM841_01605"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL88908.1"
                     /translation="MTLAEEQVRSRTVTWGDPMVSALEAFRSDGLTFLTKMAAGEIPV
                     APIASVLGFDGFEVEDGRVTFTIPVHEFHYNPIGTVHGGVLATLADSAMTCAVQTTLP
                     VGRGITTLDLSIRFLRPVTADTGRLVCVGEAIHVGRRIGTAEARITDPQGRLVAHATT
                     TCMVLEGAAQ"
     gene            808..1323
                     /locus_tag="VM841_01610"
     CDS             808..1323
                     /locus_tag="VM841_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL88909.1"
                     /translation="MGRTDFGAMNCSIARTLDVVGERWALLVLRESFFGTRRFEDFLQ
                     RLGIARNVLSDRLDRLVQAGIMRKERYQDRPERFEYRLTEKGIALNDVVLAIKAWGDR
                     WTDSAGNPPTVVRHLDCGQTFEAVPVCSECGGRVHARNVRAEPGPGATADDVAGWEER
                     LEAAAARRASR"
     gene            complement(1320..2585)
                     /locus_tag="VM841_01615"
     CDS             complement(1320..2585)
                     /locus_tag="VM841_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVL88910.1"
                     /translation="MSPSFPRATEATRAEVVIERIVAGGFGLARTADGVALVRGAMPG
                     ERVIAGLRRAKGTLTGTVLEILDANPYRDPTPLPPGADLPLSYDAQIDVKEAIVRDAL
                     QRVGGIEADIQPLRRSPRVLNYRTAAQYGVLRDGGLGAHATGSRALVPIAEDALTAEP
                     VAEAFRILSAQPMPGVRDVAIRGSLHEGRAVVGLLGEQRGPYAKIARRLVDAGIAGVA
                     WGLRTRGAARFSVASKIVAGEGDLLEDFGGILTSVSARSFAQVNPPAAGLLYSEAVEI
                     AGEGRRAIDLYAGSGVLALHLAGAFKTVDAVEISADAVKRGQNDARRLQAHGLTFRRG
                     DARTVARYLPADLVAVNPPRAGIDADTTAALIKAAPPKILYVSCDPPTWSRDVGKMVA
                     AGYRLTFARPYDFYPYTHHVEMLSLLETG"
     gene            complement(2758..3555)
                     /locus_tag="VM841_01620"
     CDS             complement(2758..3555)
                     /locus_tag="VM841_01620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVL88911.1"
                     /translation="MTEAAFFDLDKTIIARSSTLLFGRPLYREGLIARATVLKGAYAA
                     LVYQIVGADEGKMDKMRVALLELTKGWEADKIRQIARETLTELIEPIIYAEAMHLIQM
                     HRNSGRRVYLVSSSGEEIVRPLADYLGVPYAIGSKAEIVDGRYTGELTFYCQGEGKRT
                     AIQEEAAKHGIDLSRSYAYSDSATDVPMLETVGHPVAVNPDKELRKIATERGWEILVF
                     RNPITLRKRMAAVPRPAPKTALLAGAAAVGLLAAAYGYRMLAARKHA"
     gene            complement(3572..4348)
                     /locus_tag="VM841_01625"
     CDS             complement(3572..4348)
                     /locus_tag="VM841_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL88912.1"
                     /translation="MFRKPDVRLRSAWRLWQRSATIYKRTYKMSIVPNFFEPFFYLLA
                     MGLGLGAYLTRLQGVEYIDFIAPGLAASAAMFGATFEVTYNAFVKMTYGKVYDAIGTT
                     PLTPEDIALGEMLWATTRATLYGVLFVVVASLFGVVHSPLVVFVPLAVALIGMHFAVL
                     GLIYTMLIPIIDFFSYFWTMIMTPMFLFSGIFFPLDRLPEWAQTVAWFVPLHQAVNMM
                     RGLILTGDIGAAAAGAAWLLVTTAILFVVPLNLLRRKLVK"
     gene            complement(4354..5265)
                     /locus_tag="VM841_01630"
     CDS             complement(4354..5265)
                     /locus_tag="VM841_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL88913.1"
                     /translation="MNAIEASGLFKRYGAFEAVRGIDFSIAARECYGFLGPNGAGKTT
                     TMRMITGTAEPTTGTMSVLGLRVPQDGRHIRARIGVVPQENNLDEELTVIDNLIVYAR
                     YFDIPRSVAAPRADELLTFVALQDKRDWLVTKLSGGMKRRLLIARALMNKPEVLVLDE
                     PTTGLDPQARHLVWEKLRALKREGVTLVMTTHYMDEASQLCDRLVIMHEGRILIEGRP
                     RDLIAKEIPRHVIEVMDSEFTLPVRMNGLVERVEHLADRVLLYTTDGDRLLKRVRTAA
                     AEPDSVWLRGATLEDVFLHLTGRGLLE"
     gene            complement(5277..5852)
                     /locus_tag="VM841_01635"
     CDS             complement(5277..5852)
                     /locus_tag="VM841_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88914.1"
                     /translation="MATTVFLDWGLAFGAGVLFAIAGSGEVAETPRRLQTRAFRWGFA
                     YLHLGVLSISLTLYAINPDWMWMYWVDPAALPAGIQALAFVLYQICFLAGFLLTAELA
                     RRSAWLLAGLTAAAIIALEITARTRLFRFGTIDEFRAGTADPAISTNPPSIATEFWLV
                     AVAGAVSLAAMAVLLLKLVRRNPRAAPPAQA"
     gene            6178..7104
                     /locus_tag="VM841_01640"
     CDS             6178..7104
                     /locus_tag="VM841_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88915.1"
                     /translation="MAVGVYVACEDPLVADAVTHALSRTQDCFAAPALDGADVVLADP
                     LTIPEHVDAALVVLAATDPVAAARVAIARGADGIAPWPDEPASLARIVCEAAARKAPV
                     ARGRVIAVVGARGGAGATTVAAYLAARFARDTLLVDIRGGEAGQMLFAAGDGLVTLSD
                     AHGLAAEPSVKALRAMAAAHASGAPCIFGGPAPSAATPPLVACMRAASPIVVVDGAAP
                     DADATIVVCGSDVGSVRAAAALDATHVVLNMAARGRVRPRQARRALGLGEIFVVPADR
                     RIARAADLGRLARRGPGRRALMRLAARIGDAP"
     gene            7101..8288
                     /locus_tag="VM841_01645"
     CDS             7101..8288
                     /locus_tag="VM841_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005513891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HVL88916.1"
                     /translation="MIDADLRGRVQARLAERGDLLSLESRFERRIRVREAVARMLADE
                     GLALPPREIAALVHDIADSVVGLGPLEQLMRDPAVTEIMVIPSGRVYVERDGLIDDAG
                     LAMSDPRAVMHAIERIVAPLGLRIDESMPWVDARLPDGSRVHAIIPPLAVGGPALTIR
                     RFAARAITPDDMIVSGTWTAEVCEMLAACVRARANIVVSGGTGAGKTTLLNTLSSFIP
                     PGERIVTIEDAAELRLQQPHVVALQTRPPNVEGRGMVTIRDLVRNALRMRPDRIVVGE
                     VRGGEALDMLQAMNSGHEGSMSTVHANSCSDLLARIEALVLMSGAPLTVQAARRQIGA
                     GIDLILHLERGAGTRRVTEVAQVRLNEDDVVAEPVAILDGSGLVRTGIEPAVMKGRGS
                     RWR"
     gene            8279..9082
                     /locus_tag="VM841_01650"
     CDS             8279..9082
                     /locus_tag="VM841_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL88917.1"
                     /translation="MAVGRRARRLRPVARRSLSARIDRSRAGRRIAARLAESGSDEPS
                     SRFLVRTLGWTGAAAAAGWLWMGVAAAAVAAGAAIAVSAGAVRRRIRTRAVRVDEALP
                     AMLDGLSAAMRAGASLGNAIDDLEPPAVLADAVGAMRNATRFGATRREALETFARHAG
                     TERAGIVAAAITLGAESGGDLPRMLDVLAQASRDRARIAREARVASTQARMSVWVVAA
                     LPVVFLFLTGAASREQMHLLLRTPIGWGLLAAGGALEIAGLLWMRKLSP"
     gene            9079..9876
                     /locus_tag="VM841_01655"
     CDS             9079..9876
                     /locus_tag="VM841_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL88918.1"
                     /translation="MRRRARLAVARAPRDAREPREPREPRGARNARGAREARARPARY
                     ERIGARLPGVLRAGASDRLAGRRLVFASAGCLTGLALAPMPSAIVASVLLAGCGWRLA
                     GAADARAERARVEAVTEALPDALDLLAVCVIGGMGLDPALRRVSAATAGPLGESMRRA
                     VNALDAGLPWGDALKVWSERAASPAVAAVVAAMRRAARYGSPLADTLAASAAEARAAA
                     CDRAREQALAAPVRMLFPLTLCFLPAFALLAIAPSLIVAIRSLGGGP"
     gene            complement(9884..10093)
                     /locus_tag="VM841_01660"
     CDS             complement(9884..10093)
                     /locus_tag="VM841_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVL88919.1"
                     /translation="MSESIYRVTEVIGTSPTSWEDAAKNAVETAAKTLRDLRVGEISK
                     LDVTIENGQVTAYRARVGISFKYDA"
     gene            complement(10221..12803)
                     /locus_tag="VM841_01665"
     CDS             complement(10221..12803)
                     /locus_tag="VM841_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88920.1"
                     /translation="MLRRHLLLLAVVTSVLVSGFSTSASTTLTLGRQHLADNPISWDG
                     TVTRPATSRSECATVAGTPLDCTSGAVPSGTDSQTYVASLGSARRARVTTWVQWPGSD
                     SLAVWILDDQGNVVRSRSGNGSAILSYTVLNHKRYEVRVVGSVNANTQYTAYTFIRRL
                     EGQNYNGPGKLKYPTKDIFATVDIPINVVFVGFDDAEVQANKQRVLDQMPEAFRPVIR
                     IGGSRTGGDGLVQREGGPQARRLSGLSGSQIEIEPIEYRYKPRIVTASEDYSKKFFAA
                     AKAATQEGDYQLPYDREFIERYNARAAALRGINQVSPNSPIDFVDGISLEDWVAKNPP
                     AADISFDLSKPASGYTYFVVDSYRPAYAGEFFNLSRYHNFRVMNETTLDPDSGTQNGF
                     DWGRVWGGRYRFLMLDTGAAPNAWEAAHYPTTKIFRVSGNGDSSITDPPVWHYNTATI
                     GDFYDKVGEDVQFALWMRFTRGYLYRPKAYEKFILAGNTWHDADAYVPWPSKLEDLYK
                     DQLILKNYAELIPYAEWKGFSNFKYLSKGDPEQDAIDDGKRRSINGAPVPFAVSTDPV
                     MSLINRNRDTYAPLEVGAFTVPVLNVVFQSLHTFSLPAIVGGIAAGEDGEPWGQIQNV
                     NDRTKTPTATTTVKDSSGTERAPLGPDVRESLLNTVPGGVDNVARFGFTSTAFHEAGH
                     FFGLSHTHDAVAYDPAIDSDASSPTGYYDTIDWMYTTTASPMGYGWQYNKFEVLDKDN
                     IWIGHTLEWLKEAQEAIADSYAAFDVRRRTKLTAAVDWRARTAESYMNASVKALKEGR
                     YLDAVTAAKSAMRWGYWTLNMAAWTVVPGYRDAVKANATATAASAAIMPAAVMRTRRR
                     RLAA"
     gene            13006..13173
                     /locus_tag="VM841_01670"
     CDS             13006..13173
                     /locus_tag="VM841_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HVL88921.1"
                     /translation="MRLIRSERGSATAEYTLVTLVAATIALLLLNWAKGGAITSFFQS
                     IFDRVVSMFSS"
     gene            complement(13230..13739)
                     /locus_tag="VM841_01675"
     CDS             complement(13230..13739)
                     /locus_tag="VM841_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88922.1"
                     /translation="MRLRRIVAAAAVAAVALMPGSGHALGEVKRNVPGSICTVGDASL
                     AYNAVSGTWTADSNLTCHDGTASVNHPILAYELTLAHVQGVDVSLTGATLKRVPVAAS
                     GRLGCEDCWRIQGQAKRTSLVQGVYVVITQGWVTQTEGGLRYGTHETCFVVMGNNAGP
                     ATAIPGCTL"
     gene            13793..14254
                     /locus_tag="VM841_01680"
     CDS             13793..14254
                     /locus_tag="VM841_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HVL88923.1"
                     /translation="MPASWAFVLTRSPQIRGSRCRAFEEKRQILHRGSASVEFSVVAP
                     LVAIAMLVVVQVSLLAWDQMRVAHAAREGARALALANDASAAEEAALEAGRLERERAG
                     IEIAPEARPAGTAARVTVVYRPRMLVPFVGRYAPEVTLNATVWMRVERDPP"
     gene            14251..14670
                     /locus_tag="VM841_01685"
     CDS             14251..14670
                     /locus_tag="VM841_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HVL88924.1"
                     /translation="MIDARTTPPALGEDRGTVTILLCAGAFMMMLGGLAVADAGTMLM
                     LRARAQAAADAAVLAAVAEQIPALSSGQDPRAAAERHAAGNGATVVSCECEPGTATAV
                     VAVEIEARAVFLTGWAGRRIRAAAAAAADPGLLTYRR"
     gene            14708..15178
                     /locus_tag="VM841_01690"
     CDS             14708..15178
                     /locus_tag="VM841_01690"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVL88925.1"
                     /translation="MPVPREKLRLTLAEIDEVLAAERTARVATVSPDGWPHVIPMWFV
                     WRDGTIYINSLIRSRRTRDVEHGSPSSVCVDAGVNYGELRGVVLYGRFENADDDAEID
                     AIRAEFGRKYWQGIDVPAVRSHRWLKMRPERTVSWDFRKIPAGGDKRLDALEDG"
     gene            15380..16123
                     /locus_tag="VM841_01695"
     CDS             15380..16123
                     /locus_tag="VM841_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HVL88926.1"
                     /translation="MTQRESWPAEGSGYAPKTTAPRKENPVKRFGATIGFLADASRRR
                     PGGRRVLWVLVSLLAVTGVGMMTYPFATQIWANQIQKDKAKQFASSELLNAYKTDTVG
                     AAVGEGEALTRLQIPKLKVDVIVVEGVSGNALRAGAGHYPESALPGDVGNVAIAGHRT
                     GFGQPFRDLDRLKAGDTIILTTPLGNYTYRVMPPFDGHPNPWITHPLDWTVIAPTPTP
                     VLTLTTCDPPHTSKNRLIVRAELVDTQES"
     gene            complement(16197..17411)
                     /locus_tag="VM841_01700"
     CDS             complement(16197..17411)
                     /locus_tag="VM841_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL88927.1"
                     /translation="MPAKKKAAAAKKALPKKAPGKKTPRTAASAKTPAPKKKQAARKN
                     PAKKPAAARPRVRRDDDLPPELAIEHEGAIPDAVLAAAQRTTERQLASREVRRDAAKE
                     RREAIGTEEPEAALTRLAAASVPGAVVEERMAHETHDTLTINEPEPISGVRVAPAGPA
                     PARDRGGVVIEVENLHVFYGAVQALRGVSFRVHEGETVAIIGSNGAGKTTTLKTVSGV
                     SELHKTVRGHITIMGRRVETWPAHKIARLGVAHSPEGRKVFPNQTVRDNLMLGAFGRR
                     DADVERDIRRMEERFPILGERRSQPAGLMSGGEQQMLAMARALMARPRVLLFDEPSMG
                     LAPLIVKQIFEIIQELKREGITILLVEQMAFQALAIADRGYVLETGEITMEGSGITLL
                     EDRRVMEAYLGA"
     gene            complement(17420..19492)
                     /locus_tag="VM841_01705"
     CDS             complement(17420..19492)
                     /locus_tag="VM841_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVL88928.1"
                     /translation="MLDRLKEANRTAVAERDRRRALKAWTRARAAGAPAAARPPSVLP
                     EAAAEMALRPEPRLSVFATPGGIAFTLLLIALLAWPFALPGFDPSTFLEVQKILAATI
                     VVIGLNLIAGFTGQLSLGQKIFVLIGGYTVGVMTTRFDSSLLGNHWISLVVAIALGAA
                     AGAMLGLPALRVKGAYLSIVTLAFVPIFYTLFNSELLKPVFGGAQGIGDIATPIGNIA
                     RPPNRTINIPFTGTAMTDVDYYLVLVLIFGLLFFLARNIVKSRWGRAMTAVRESEIAA
                     KSSGVRVYKVKVAAFMVSAAYASLAGALLTSFSTAQISPESAAAVHIRDSFVYVVMMI
                     IGGTGTLAGPLVGAALVSLIRLLIPKVGEILDFDLKRWTTTLLAILALVSVVAAPGGT
                     VGIINDRIHRRRTRHRYRNPPPRPPALPASLVRPTPREDAATGDVVLETREMSKIFGG
                     LRANDSVSIRVERGTVHSLIGPNGSGKTTFINVVTGVYAPEEGSVYFAGERIDGQPAF
                     ITVERGMGRTFQNLQLWRRMTVLENVMVGMHVRVGSGFIGNMVRTGRSRREEARIKAR
                     AMGLLQFVGLEDVADQLAGTLPYGKQRYLEIARALALDPVLLILDEPAAGLNPAEVHD
                     LMDLIRRIQRTGITVFLIEHHMDLVMGVSDRISVLDYGSKIADGTPAEIQADQRVVEA
                     YLGAEAHV"
     gene            complement(19503..20396)
                     /locus_tag="VM841_01710"
     CDS             complement(19503..20396)
                     /locus_tag="VM841_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL88929.1"
                     /translation="MEFLQAIVLGLTTGGVYALIALAFSIIYMTTRVINFAQGEVLMI
                     GAYLYFFAVSSGGNALGLPWPLALVIALFGATIVGLVVHLISIAPLGKFDPNTNIGWI
                     LTTLSVSLIFVEVVRNLAGEQAKRVEPVISSIGGKSTVFSIEPKQYLVLVVGLALAVV
                     LEVVHSKTQLGRALRATAHDKATASLMGINTGFMVRYSFALAGLLAGVGGWLLGPIIF
                     VSADMGTLVGLTAFVAAVVGGIGSTRGALVGGLTIGVVESLAKQAGAGEWTSALVFLT
                     LIVILVVKPTGILGEALIEKV"
     gene            complement(20406..22205)
                     /locus_tag="VM841_01715"
     CDS             complement(20406..22205)
                     /locus_tag="VM841_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL88930.1"
                     /translation="MTRREPRHADEWDARPAEPAPPRPGEPVPSYEQPARTPRNRREA
                     ARWEREMEERSKLGWDTPINRRAFLDLSKRLGLGAAAASAAFPAFLAACGRTASSLSP
                     SRGAKFDVGGGADGDTINIGAISIYSGVGAFVGKLLDRGAGLATKQINRHGLPDPAIM
                     SSRNGFPDFEAYRKQDLGGIKIGDRTVKLRLITRDDNLSAQVAVSAIQEMITSFNIKG
                     LIFAGLYDDIYAAKRLLQQYNLPAIAAYGDIASVGQLYPQTDYRQLFQMFPPDKWGIE
                     IALEEYSIADRGYRRFAYLGDNTAIGAQGKRLIGETLAKSGNRLLAAEQYNVGDVDMT
                     AQLQRIRSTNPHVLMIWGIAGDTAHALESLKRLDAVYLNRTTALRGPGWHPQIIGFEG
                     GAAERTFAVLAGDAARVGTMSYWYMGGIGYIPEFQPAVDLFKEEFGDSPTGGENNPAD
                     AVFLFAKAFQDAQSTDGDKVIAALESMEKGINFSSIAPHTFSKDRHISLEKDDMVGIT
                     LERGRAAPADPPYTLGTEFTGNDDLGIKPFNPPGYIGPTMFMRFNQDGLRRKHRKLFV
                     DVMLKQGFGTQCTKRPDPGSDWGFRLTNECDIH"
     gene            complement(22202..22447)
                     /locus_tag="VM841_01720"
     CDS             complement(22202..22447)
                     /locus_tag="VM841_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88931.1"
                     /translation="MTHRMADAFVRTRVALMLVVMTALAWTVVTLMGRGTGPYRAGEL
                     PDLAGLPILVPQIERLAPPRDLMPGAPAPAPPPEGSP"
     gene            complement(22444..23070)
                     /locus_tag="VM841_01725"
     CDS             complement(22444..23070)
                     /locus_tag="VM841_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HVL88932.1"
                     /translation="MNLAARAPAAAGAAPRLVLHRWQAIALATLVPALLSGLYVSTNV
                     RASLAQRSLRAQWEPAVAAGPVAPATLAARAFTPGEPVARLGIAATGADLIVVAGGNG
                     ARQGPALAAGSAYPGLPGVSVIEAGRFGYGNMFLHLDRLIPGDTIVVETLAGVRRFTV
                     RSVRVLPASAIDPREDSVTPVLLLAAPARAWGSSDRIVVRAESAGAAR"
     gene            complement(23067..>23261)
                     /locus_tag="VM841_01730"
     CDS             complement(23067..>23261)
                     /locus_tag="VM841_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88933.1"
                     /translation="ALGTFDRVWTPQDWYKNQVAPPGFRLPGGIASVGATALLTGNLL
                     MFWWLRRRGDISVEVREVGR"
BASE COUNT         3520 a         7910 c         8131 g         3700 t
ORIGIN      
        1 gtccagcgcg acggctgacc gcagccggca gcggggccgt cgccgattcg cggcatgggg
       61 accatgaagc ggcggtagcg cgtctcggac gagcttcgct cgagcatcgc gcgcatctcg
      121 gccatgtccg tgggtgcgac cgctcgggtg acgacgggaa ggagcgtggt cgtgatcgcg
      181 tgagcgaacg ctgcggtcaa cggagtcatt gggcagctcc ttccagcacc atgcaggtcg
      241 tcgttgcgtg tgcgacgagc cgtccttgcg gatccgtgat gcgcgcttcg gcggtgccga
      301 tgcgccgccc gacgtgaatc gcttcaccga cacagacgag acggcccgtg tccgccgtga
      361 cgggtcgaag aaagcgaatc gaaagatcca aagtggtgat gccccggccg acgggaagcg
      421 tggtttgcac cgcgcatgtc atggccgaat cggccagcgt cgcgagcacc ccgccgtgaa
      481 cggttccgat cgggttgtag tggaactcgt ggacgggaat ggtgaaggtg acccggccgt
      541 cctcgacctc gaagccgtcg aagcccagga ccgaggcgat cggggcgacc gggatctcgc
      601 cggcggccat cttcgtcagg aacgtgagcc cgtccgaccg gaaggcctcg agggccgaaa
      661 ccatcgggtc gccccaggtc accgtccggc ttcgaacctg ctcttcggcc agcgtcatcg
      721 cgacacccct ttcgtcagtt tcaagttgca acccacttgt agcatggtcc gttgcaaact
      781 gcaactcact tcgatagagt ggtttccatg ggacggacgg acttcggcgc gatgaactgc
      841 tcgatcgccc gtaccttgga cgtggtgggg gagcggtggg ccttgctcgt gctgcgggag
      901 tcttttttcg gtacccgccg cttcgaggat ttcttgcagc ggctgggcat cgcccgcaac
      961 gtcctgtccg accgcctcga ccgtctggtc caggcgggca tcatgcgcaa ggagcgctac
     1021 caggaccgtc ccgagcgctt cgagtaccgg ctgacggaaa agggcatcgc gctcaacgac
     1081 gtcgtgctgg ccatcaaggc ttggggtgac cgatggaccg actcggccgg caacccgccg
     1141 acggtcgtgc ggcacctgga ctgcggccag acgttcgagg ccgttccggt gtgctcggag
     1201 tgcggcggtc gcgtgcacgc gcgcaacgtt cgtgccgagc ccggtccggg cgcgacggcc
     1261 gacgacgtcg ccggttggga agagcgcttg gaagcggccg cggcgcgtcg cgcgtcgcgc
     1321 tagccggtct ccagcagcga cagcatctcg acgtggtgcg tgtacggata gaaatcgtag
     1381 ggccgcgcga acgtcagccg gtatcccgcc gcgaccatct tgccgacgtc gcgtgaccac
     1441 gtcggcggat cgcacgacac gtacaggatc ttgggcggcg cggccttgat cagcgccgcg
     1501 gtcgtgtccg cgtcgatgcc tgcgcgcggc ggattgaccg caaccagatc ggccggaagg
     1561 tagcgcgcga ccgtgcgcgc gtcgcctcgg cgaaaggtca gcccgtgcgc ttgcagccgc
     1621 cgcgcgtcgt tttggccgcg cttgaccgca tcggcggaga tctcgaccgc atccaccgtc
     1681 ttgaaggccc cggccagatg aagcgcgagc acgccgctgc ccgcatacag gtcgatcgcg
     1741 cgccgtccct cgccggcgat ctcgacggcc tcgctgtaga gcaggcccgc cgcgggcgga
     1801 ttgacctgcg cgaagcttcg ggcgctcacg ctggtcagga tgccgccgaa gtcctcgagc
     1861 agatcgccct cgccggccac gatcttcgaa gccacgctga accgcgccgc gccgcgcgtc
     1921 cgcagccccc acgcgacgcc cgcgatcccc gcgtccacga gccgccgggc gatcttcgcg
     1981 taggggccgc gctgttcgcc cagcaaaccc accacggccc ggccttcgtg caggctgccg
     2041 cgaatcgcga cgtcgcgcac gccgggcatc ggctgcgcgc tcaggatccg gaacgcctcc
     2101 gcgaccggct cggccgtcaa cgcgtcttcg gcgatcggaa cgagcgcgcg gctgccggtc
     2161 gcgtgtgcgc cgagcccgcc gtcgcgaagc accccgtatt gcgcggcggt ccggtagttg
     2221 agcacgcgag gtgaacggcg gagcggctga atgtccgcct cgattccgcc gacgcgctgc
     2281 agcgcgtcgc gcacgatcgc ctctttgacg tcgatctggg cgtcgtagga aagcggcagg
     2341 tcggcgccgg gcggaagcgg cgtcggatcg cggtagggat tcgcgtcgag gatttccagc
     2401 accgtccccg tcagcgtccc tttcgcgcgt cgcagccctg cgatcacgcg ctcgccgggc
     2461 atcgcgccgc gcaccagggc gacgccgtcg gccgtgcgcg ccaagccgaa tccgcccgcg
     2521 acgatgcgct cgatgacgac ctcggctcgc gttgcttcgg tcgcgcgcgg aaatgaaggg
     2581 ctcatgtggg tgggaggata aggaagggag ggtcggctaa caaggcgcgc tcgccaatgt
     2641 cggggtcatt gccccggact cgcctcccgc ggctgaccgc gcgtgggtac cgtccctccc
     2701 ttgcgcatct tcgatgctac acccggccgt gggaacggtc caggagaggg ctggaagtca
     2761 cgcatgcttg cgagcggcaa gcatgcggta gccgtaggca gcggcgagaa ggccgaccgc
     2821 cgcggcgccc gcgagcaatg cggtcttggg cgcggggcgg ggaacggcgg ccatgcgttt
     2881 gcgaagcgtg atcgggttcc ggaacacgag aatctcccag ccgcgctccg tcgcgatctt
     2941 gcggagctcc ttgtcgggat tcacggcgac gggatgccca acggtctcga gcattggaac
     3001 gtcggtcgcg ctgtcgctgt aggcgtacga gcgcgacagg tcgatgccgt gctttgcggc
     3061 ttcttcctgg atcgcggtgc gcttgccttc cccctggcag tagaacgtga gctcgccggt
     3121 gtagcggcca tccacgatct cggccttcga gccgatcgcg tacgggacgc ccaggtaatc
     3181 ggccagcggc cgcacgatct cctcgcccga ggacgacacc agatagaccc gccggccgct
     3241 gttgcggtgc atctgaatta agtgcatcgc ttcggcgtag atgatcggct cgatcagctc
     3301 ggtcagcgtc tcgcgcgcga tctggcggat cttgtcggcc tcccagccct tcgtcagctc
     3361 gagcagcgcg acccgcatct tgtccatctt gccttcgtct gcgccgacga tctgatagac
     3421 gagcgccgcg tacgcgccct tgagcacggt cgcgcgagcg atcaggcctt cccgatacag
     3481 cgggcggccg aacagcaacg tggacgaccg ggcgatgatc gtcttgtcga ggtcgaagaa
     3541 ggcagcttcc gtcatgacgg atgaatggta cctacttcac gagcttccgg cgcagaaggt
     3601 tcagcggcac gacgaatagg atcgcggtgg tgaccaggag ccaggccgca ccggccgctg
     3661 cggcgccgat atcgccggtc aggatcaggc cgcgcatcat gttgacggcc tgatgcagcg
     3721 gcacgaacca ggcgacggtc tgcgcccatt ccggcagccg gtccagcggg aagaagatgc
     3781 ccgagaacaa gaacatcggc gtcatgatca tcgtccagaa gtacgagaag aagtcgatga
     3841 tcggaatcag catggtgtag atcagcccca ggaccgcgaa gtgcatgccg atcaacgcga
     3901 ccgcaagcgg gacgaagaca accagcggcg agtgcaccac gccgaacagc gaggccacca
     3961 ccacgaacag cacgccgtaa agcgtcgcgc gtgtcgtcgc ccagagcatc tcgcccagcg
     4021 cgatgtcctc cggcgtcagc ggcgtggtgc cgatcgcgtc gtagaccttg ccgtaggtca
     4081 tcttcacgaa cgcgttatag gtaacctcga acgtcgctcc gaacatcgcg gccgacgcgg
     4141 caaggccggg cgcaatgaag tcgatgtatt cgacgccttg cagccgcgtc aggtaggcgc
     4201 ccaggccgag gcccatggcc agaagataga agaacggctc gaagaagttg gggacgatgc
     4261 tcatcttgta ggtgcgcttg tagatcgtgg cgctgcgctg ccacagccgc cacgctgagc
     4321 gcagccgcac atcgggtttg cggaacacgg tgatcattcc agcagcccgc gcccggtcag
     4381 atgaaggaag acgtcttcca gcgtcgcgcc gcgaagccag acgctgtcgg gctcggcggc
     4441 ggcggtgcga acgcgtttga gcagccggtc gccgtcggtg gtgtagagca gcacgcggtc
     4501 ggccaggtgc tcgacgcgtt cgaccagacc gttcatgcgc acgggcagcg tgaactcgga
     4561 atccatgacc tcgatgacgt gccgggggat ctccttggcg atcaggtcgc gcggccggcc
     4621 ttcgatcagg atgcggccct cgtgcatgat caccagccgg tcgcacagct gcgaggcctc
     4681 gtccatgtag tgcgtcgtca tcaccagcgt cacgccttcg cgcttgagcg cgcgcagctt
     4741 ctcccacacc agatgccgcg cctgaggatc caggccggtg gtcggctcgt ccaggacgag
     4801 cacctcgggc ttgttcatca gggcccgggc gatcagcagc cggcgtttca tccctcccga
     4861 tagtttcgtc accagccagt cgcgcttgtc ctgcagcgcg acgaaggtca gcagctcgtc
     4921 ggcgcgcggc gcggcgacgc tgcgcgggat gtcgaagtag cgcgcgtaga cgatcaggtt
     4981 gtcgatgacg gtcagctctt cgtcgaggtt gttctcttgc gggaccactc cgatgcgcgc
     5041 gcgaatgtgg cggccgtctt gagggacgcg cagacccagg acgctcatcg ttccggtcgt
     5101 gggctcagcg gtgccggtga tcatgcgcat cgtggtggtc ttgccggctc cgttcgggcc
     5161 gaggaagccg tagcattcgc gcgcggcgat cgaaaagtcg ataccgcgca cggcctcgaa
     5221 ggcgccgtag cgtttgaaca ggcccgaggc ctcgatggca ttcacggcag gcgagtttac
     5281 gcctgcgcgg gcggcgcggc gcgcggattc cggcgcacca gcttcagcag gagcaccgcc
     5341 atcgcggcga gcgagaccgc gccggccact gcaacgagcc aaaactcggt cgcgatcgac
     5401 ggcggattgg tcgagatcgc cggatcggcc gtgccggccc ggaactcgtc gatcgtgccg
     5461 aagcggaaca ggcgcgtgcg cgcggtgatc tcgagcgcga tgatcgcggc ggcggtcagt
     5521 ccggccagca gccatgcgct gcgccgggcc aactcggcgg tcagcaggaa gccggccaag
     5581 aagcagatct ggtagaggac gaacgcgagc gcctggatgc cggccgggag cgccgcgggg
     5641 tctacccagt acatccacat ccagtcgggg ttgatcgcgt agagcgtgag cgagatcgac
     5701 agcacgccga ggtgcagata ggcgaaaccc cagcggaatg cgcgcgtctg cagccggcgc
     5761 ggggtttcag caacctcacc gctgcccgcg atcgcaaaca gcacgccggc gccgaacgcc
     5821 agcccccaat ccagaaagac ggttgtggcc acctccttcg gcctcgattg tagggcggcc
     5881 ggcgccgatc cttcggacgt tcgccgcgat ccggtccgaa cccatgtttt cgtgccggca
     5941 tcgaggtatc gattcggagg gcgaccgcga ttcctgcttg gtgggaagaa cggccaaggc
     6001 ggagcgctat ggcaaggtgt cggggagcct cggacgattt gcgcaacgac atcccaacgt
     6061 ggccgtcgtg gagatctgaa gcgctcgtac cggctcgatc cggatcttcg agattctgtt
     6121 cacaatcgag tctgcgccgc tggaagtcag ggtgtcttcg tcgtagcgtc gcgcgacatg
     6181 gcggtcggtg tgtatgtcgc gtgtgaggat ccgctcgttg cggatgcggt gacgcatgcg
     6241 ctctcgcgta cccaggactg cttcgcggca ccggcgctcg acggtgcgga cgtggtcttg
     6301 gccgatccgt tgacgattcc tgaacacgtc gatgccgcgc tcgtcgtgct tgcggcgacc
     6361 gatccggttg cggctgcgcg agtcgcgatc gcgcgaggcg cagatgggat cgcgccctgg
     6421 ccggacgagc cggcctcgct ggctcgcatc gtctgcgaag cagccgcgcg gaaggcgccg
     6481 gtcgcacgtg gacgggtgat cgccgtcgtc ggcgcgcgcg gcggagcagg tgcgacgacg
     6541 gtcgcggcgt acctcgctgc gcgattcgca cgggacacgc tgctggtgga catccgcggc
     6601 ggcgaggccg gtcagatgtt gttcgcggcc ggtgacgggc tcgtcacgct gtcggacgcg
     6661 cacgggctgg cggccgagcc ttcggtgaag gccctgcgcg ccatggcggc cgcacatgcg
     6721 agcggcgcgc cgtgcatctt cggcgggccc gcgccgtcgg ccgccacccc gccgctcgtt
     6781 gcatgcatgc gggccgcttc gccgatcgtg gtcgtcgacg gcgcggcccc cgatgccgac
     6841 gcgacgatcg tggtgtgcgg ttccgacgtc ggatcggtgc gcgcagccgc agcgctggac
     6901 gcaacgcacg tggttttgaa catggcggcg cgtggacggg tgcgtccgcg ccaagcgcgc
     6961 cgcgcgctgg gcctaggcga gatcttcgtc gtgccggccg ataggcgcat cgcccgtgcg
     7021 gcggacctcg ggcggctcgc gcggcgcggg ccgggtcgcc gcgcgctcat gcgactcgcc
     7081 gcacggatcg gggatgcgcc gtgatcgacg cggaccttcg cggccgcgtc caggcgcggc
     7141 tcgccgaacg cggagatctg ctgtcgctgg agtcgcggtt cgagcggcgc atccgcgtgc
     7201 gcgaggcggt ggcgcggatg ctcgccgacg agggtcttgc gcttccgccg cgcgagatcg
     7261 cggccctggt gcacgacatc gcggactcgg tcgtggggct cgggccgctc gagcagctga
     7321 tgcgcgaccc ggccgtcacc gagatcatgg tcatcccgtc cggccgcgtc tacgtcgagc
     7381 gcgacggcct catcgacgac gccggtttgg cgatgagcga cccgcgggcg gtcatgcatg
     7441 cgatcgaacg gatcgtggcg ccgctcgggc tgcgcatcga cgagtcgatg ccgtgggtcg
     7501 atgcccgcct gccggacggc agccgcgtcc acgcgatcat cccgccgctg gcggtcggcg
     7561 ggccggcgct gacgattcgc cgcttcgccg cccgcgcgat cacgcccgac gacatgatcg
     7621 tctccgggac atggacggcc gaggtgtgtg agatgctcgc tgcctgcgtc cgggcccgcg
     7681 ccaacatcgt cgtgtcgggc ggcaccggcg ccggaaagac cacgctgctc aacaccctgt
     7741 cgtcgttcat ccctccgggc gagcggatcg tcacgatcga agacgccgcg gagcttcgcc
     7801 tgcagcagcc gcacgtggtc gccctgcaga cgcggccgcc caacgtggag ggccgcggca
     7861 tggtcacgat ccgcgacctg gtccgcaacg cgctgcgcat gcggcccgac cggatcgtcg
     7921 tcggcgaggt tcgcggcggc gaggcgctcg acatgctgca ggcaatgaac agcggacacg
     7981 aaggatcgat gagcaccgtg cacgccaact cgtgcagcga tctgctcgcg cgcatcgagg
     8041 cgctggtgct gatgagcggc gctccgctga ccgtgcaggc cgcgcgccgg cagatcggcg
     8101 ccggcatcga cctgatcctg cacctggagc gcggcgccgg cacgcgccgc gtcaccgagg
     8161 tcgcccaggt gcgtctgaac gaagacgacg tcgtcgccga gccggtcgca atccttgatg
     8221 ggtcggggct ggtgaggacg ggcattgagc cggccgtcat gaaaggccgc ggttcgcgat
     8281 ggcggtaggc cgccgagcca gacgtcttcg gccggtcgcg cggcgaagcc tgtccgcccg
     8341 gatcgaccgc tcgcgcgccg ggcgccggat cgccgcgcgc ctggccgagt ccggcagcga
     8401 cgagccttcg tcgcggttcc tggtccgcac gctcggatgg acgggcgcgg ccgcggccgc
     8461 gggctggctc tggatgggcg ttgcggccgc cgcggtcgcg gccggcgcgg cgatcgccgt
     8521 ctccgcaggc gccgtgcgac ggcgcatccg aacgcgcgcc gtccgcgtcg acgaagcctt
     8581 gcccgcgatg ctcgacggcc tctcggcggc gatgcgcgcc ggcgcctcgc tcggaaacgc
     8641 gatcgacgat ctggagccgc ccgccgtgct ggccgacgcg gtcggggcga tgcggaacgc
     8701 gacgcggttc ggcgcgaccc gccgcgaggc gctcgagacg ttcgcgcggc acgcgggaac
     8761 cgagcgggcc ggaatcgtgg ccgctgcgat cacgctcggc gccgaaagcg gcggcgatct
     8821 cccgcggatg ctcgacgtgc tcgcgcaggc ctcgcgcgac cgggcccgga tcgcgcgaga
     8881 ggcgcgcgtc gccagcaccc aggcgcgcat gtccgtctgg gtcgtcgcgg cgctgccggt
     8941 cgtgttcctt ttcttgaccg gagccgcatc gcgcgagcag atgcatctgc ttctgcgaac
     9001 cccgatcggt tgggggctgc tcgccgccgg cggggcgctc gagatcgccg gcctgctctg
     9061 gatgagaaag ctgtcgccat gagacgccgc gctcgcctgg ccgtcgcgcg tgcgccgcgc
     9121 gatgcccgcg agccgcgtga gccccgcgag ccgcgtgggg cgcgaaatgc gcgtggggcg
     9181 cgtgaggcgc gcgcgaggcc cgcgcgttac gagcggatcg gcgctcggct gccgggcgtg
     9241 ctccgggccg gagcgtcgga tcgcctggcc ggacggcgcc tggtcttcgc atcggccggg
     9301 tgcctcacgg gactggcgct cgcgcccatg ccatcggcga tcgtggcgtc ggtgctgctc
     9361 gccggctgcg gatggcggct cgccggcgct gccgacgcgc gcgccgaacg cgcgcgcgtc
     9421 gaggccgtga ccgaggcctt gcccgacgcc ctggacctgc tggccgtatg cgtgatcggc
     9481 ggcatgggcc tggatcccgc gctgcggcgc gtatccgccg ccaccgcagg accgctcggg
     9541 gaaagcatgc gccgcgcggt caacgcgctc gacgcgggac tgccttgggg cgacgcgctc
     9601 aaggtctgga gcgagcgcgc ggcgtccccc gccgtcgccg cggtcgtcgc ggcaatgcgc
     9661 cgcgccgcgc gctacggatc gccgctggcc gacacgcttg ccgcgtcggc cgctgaggcg
     9721 cgcgccgcgg catgcgaccg ggcgcgcgag caggcactcg ccgcgcccgt ccgcatgctt
     9781 ttcccgttaa cgctgtgctt cctgcccgcc ttcgcgctgc tcgcgatcgc cccgtcgctg
     9841 atcgtggcga tccgctcgct cggcggcggg ccctaggacg cgatcaggcg tcgtacttga
     9901 acgagattcc cacgcgcgcc cggtaggcgg tgacctggcc gttctctatg gtcacgtcca
     9961 gcttcgagat ctcgccgaca cggagatcgc gcagcgtctt ggccgccgtc tccaccgcgt
    10021 tcttggcggc gtcctcccac gaggttggac tcgttccgat cacttcggtg acgcgataaa
    10081 tgctctcgga catcccggtc tcccttcagg ttgcggctga tgcgtttcct gccgtcgatc
    10141 ctacgcggcg ccgggccccc cgcgaaaggg accggccggt cccaactttc gggaccggcc
    10201 gatgagaacg ggcgaccgtg ttacgcggcg agacggcggc ggcgcgtgcg catcaccgcg
    10261 gccggcatga tcgcagccga agcggccgtc gcggtcgcgt tggccttcac cgcgtcgcgg
    10321 tagccgggca cgaccgtcca tgcggccatg ttgagcgtcc agtagcccca gcgcatcgcg
    10381 ctcttggccg cggtgacggc gtcgaggtag cggccttcct tcagcgcctt gaccgaggcg
    10441 ttcatgtacg actcggccgt gcgagcccgc cagtccaccg cggcggtcag cttcgtgcgt
    10501 cgacgaacgt cgaaagccgc gtacgagtcc gcgatcgctt cttgcgcctc cttcagccac
    10561 tccagcgtgt gcccgatcca gatgttgtcc ttgtcgagca cctcgaactt gttgtattgc
    10621 cagccgtagc ccatcgggct cgcggtggtc gtgtacatcc agtcgatcgt gtcgtagtag
    10681 ccggtcgggc tcgacgcgtc gctatcgatg gcggggtcgt aggcgaccgc gtcgtgcgtg
    10741 tgcgaaaggc cgaagaagtg cccggcctcg tggaacgccg tcgatgtgaa gccgaaccgt
    10801 gcgacgttgt ccacgccgcc cggaaccgtg ttcagcagcg actcgcgcac atcggggccc
    10861 agcggcgcgc gctcggttcc cgacgagtcc ttgaccgtgg tagtggcggt cggcgtcttg
    10921 gtgcggtcgt tgacgttctg gatctgaccc cagggctcgc cgtcttcgcc ggcggcgatg
    10981 ccgcccacga tcgccggaag cgagaacgtg tgcaggctct ggaacaccac gttcagaacc
    11041 gggaccgtga acgccccgac ctcgagcggc gcgtacgtgt cgcggttccg gttgatcagg
    11101 ctcatcaccg gatcggtgct gaccgcgaac ggaaccggcg ccccgttgat ggagcggcgc
    11161 tttccgtcat cgatcgcgtc ctgctccgga tcgcctttcg acagatactt gaagttcgag
    11221 aaacccttcc actccgcgta gggaatcagc tcggcgtagt tcttgaggat cagctgatcc
    11281 ttgtagaggt cctcgagctt cgagggccac ggcacgtacg cgtcggcgtc gtgccaggtg
    11341 ttgccggcga ggatgaactt ctcgtaggcc ttcggacggt acagataacc gcgtgtgaag
    11401 cgcatccaca gcgcgaactg cacgtcctcg ccgaccttgt cgtagaagtc gccgatggtg
    11461 gccgtgttgt agtgccacac gggcggatcg gtaatcgacg aatcaccgtt gccgctgacg
    11521 cggaagatct tcgtggtcgg gtagtgcgcg gcctcccagg cgttcggggc cgcgccggtg
    11581 tcgagcatca agaaccggta gcggccgccc cacacgcggc cccagtcgaa gccgttctgc
    11641 gtgcccgagt ccggatccag cgtggtctcg ttcatcacgc ggaagttgtg gtagcggctc
    11701 aggttgaaga actcgcccgc gtacgcgggc cgatacgagt ccacaacgaa gtaggtgtag
    11761 ccgctggcgg gcttcgacag gtcgaagctg atgtcggcgg ccggcgggtt cttggcgacc
    11821 cagtcctcga gcgagatgcc gtcgacgaag tcgatcgggg agttgggcga gacctggttg
    11881 atgccgcgca gcgccgccgc gcgcgcgtta tagcgctcga tgaactcccg gtcgtagggg
    11941 agctggtagt cgccttcctg ggtcgcggcc ttcgcggccg cgaagaactt cttgctgtag
    12001 tcctcgctcg ccgtgacgat gcgcggcttg tagcgatact cgatcggctc gatctcgatc
    12061 tggcttccgc tcaggccgct gagccggcgg gcctgcgggc cgccttcgcg ttgcacgagc
    12121 ccgtcgccgc cggtgcgcga gccgccgatg cggatcacag gccggaacgc ctcgggcatc
    12181 tggtcgagca cgcgctgttt gttggcctgc acctcggcgt cgtcgaatcc gacgaacacc
    12241 acgttgatcg gaatgtcgac cgttgcgaag atgtccttgg tcggatactt cagcttgccc
    12301 gggccgttgt agttctggcc ttcgaggcgg cggatgaaag tgtaggcggt gtactgcgtg
    12361 ttcgcgttga cgctgccgac cacacgcacc tcgtagcgct tgtgattgag cacggtgtag
    12421 gacaggatcg ccgagccgtt gccgctgcgg gagcgaacga cgttgccctg gtcgtccagg
    12481 atccacaccg cgagcgagtc cgaccccggc cactggaccc aggtcgtcac tcgtgcgcgc
    12541 cgcgcgctgc ccagcgaggc gacgtaggtc tgcgagtccg tcccgctcgg gacggcgccg
    12601 ctggtgcagt ccagcggcgt gcccgctacc gtcgcgcact cgctgcgcga ggtcgccgga
    12661 cgcgtcaccg ttccgtccca ggagatgggg ttgtcggcca gatgctgccg tccgagcgtg
    12721 agggtcgtcg acgccgacgt cgagaacccc gagacgagca cggacgtaac cacggctaac
    12781 agcagcagat gccggcgcag cattcggatc ctcctaaagg gtttcggtgg gttccttcat
    12841 atatacgagg tccggggcct cgatgccttc tgcgaccggt tccccggttc gggacgcccc
    12901 ggtctgggcg atcgaagccg atgggcgaga tttgcctgtt tcgccgggaa tggaggcctg
    12961 gggcaaggcg cagtagcgtc gcggctcccg atgaaaggag acccgatgcg cctcatccgc
    13021 agcgaacgcg gctccgcaac cgccgagtac acgctcgtca cgctggtggc cgccacgatt
    13081 gctctgctgc ttctgaactg ggccaagggc ggcgcgatca cgtccttctt ccagagcatc
    13141 ttcgatcgcg tcgtttcgat gttctcgtcg tgaggcgagc aaagcgaagg gggcgcggca
    13201 ggatcgccgc gcccccggaa aaggccacgc tacagagtgc agcccgggat cgcggttgcc
    13261 gggccggcgt tgttgcccat gacgacgaag cacgtctcgt gcgtgccgta gcgcaggcca
    13321 ccctcggtct gcgtcaccca cccctgcgtg atgacgacat agacgccttg cacgagcgac
    13381 gtccgcttcg cctgtccctg gatccgccag cagtcctcgc aacccaggcg gccgctcgcg
    13441 gccacgggaa cccgcttgag cgtggctccg gtcagcgaga catcgacccc ttgcacgtgc
    13501 gccaacgtca gctcgtacgc cagaatcggg tggttcaccg acgcggtgcc gtcgtggcag
    13561 gtgaggttcg agtcggcggt ccaggtcccg ctcacggcgt tgtaggcgag cgaggcgtcc
    13621 ccgaccgtgc agatgctgcc cggcacgttc cgcttcacct cgccgagagc atggccggac
    13681 ccgggcatca gcgcaacggc cgcgacggcc gcagcggcga cgatacgacg caaacgcata
    13741 cgctcctccc ccttcggatg gttttgggca agacttcaac caatgaggcg gaatgcctgc
    13801 atcgtgggcg ttcgtgttga cccgttcgcc gcaaatccgc ggatcaaggt gtcgggcctt
    13861 tgaggaaaag cggcagatcc ttcatcgcgg gtcggcgtcc gtcgaattct ccgtcgtggc
    13921 gccgctggta gcgatcgcca tgctcgtggt ggtgcaggtc tcgctgctgg cgtgggatca
    13981 gatgcgcgtt gcgcacgccg cacgcgaagg cgctcgcgcg ctcgcgctgg ccaacgacgc
    14041 ctccgccgcc gaagaggccg cgctcgaagc cgggcggctc gaacgcgagc gggccggcat
    14101 cgagatcgcc cctgaggctc ggcccgccgg gacggctgcg cgcgtcacgg tcgtttaccg
    14161 tccgcgaatg ctggtgccgt tcgtgggccg ctacgcaccc gaggtcaccc tgaacgcgac
    14221 ggtgtggatg cgcgtggagc gggacccgcc gtgatcgatg cccgaacgac gccaccggcc
    14281 ctcggcgagg accggggcac ggtcacgatt ctgttgtgcg ccggggcatt catgatgatg
    14341 ctcggcggcc ttgccgtcgc cgacgccggc acgatgctga tgctccgcgc gcgcgcacag
    14401 gccgccgcgg acgcggccgt gctcgcggcc gtggccgagc agattcccgc gctttcctcg
    14461 ggccaagacc cgcgggccgc cgctgagcgg cacgccgccg gcaacggagc aaccgtggtc
    14521 tcgtgcgaat gcgagccggg cacggcgacg gccgtcgtcg ccgtcgagat cgaagcccgg
    14581 gcggtgtttc taaccggctg ggccgggcgc cggattcgtg ccgccgcggc cgccgcggcc
    14641 gatcccggcc tcctcaccta ccgtcgctga accgcgcggc tgcggcggcg cgcggtaggc
    14701 ttcggccatg cccgtcccac gcgaaaagct tcgcctcacg ctggccgaga tcgatgaggt
    14761 cctcgctgcc gaacgcaccg cgcgggtcgc caccgtctct cccgacggat ggccgcacgt
    14821 gatcccgatg tggttcgtct ggcgcgacgg cacgatctac atcaactcgc tgatccgctc
    14881 gcggcgcacc cgcgacgtcg agcacggctc gccgtcttcg gtgtgcgtcg atgcgggcgt
    14941 gaactacggc gagctccgcg gggtcgtgct gtacggacgc ttcgagaacg ccgacgacga
    15001 tgccgagatc gacgcgatcc gcgcggagtt cggccgcaag tactggcagg gtatcgatgt
    15061 gcctgccgtg cgcagccacc gctggctgaa gatgcgcccg gaacgcaccg tctcgtggga
    15121 tttccgcaag atcccggcgg gcggggacaa gcgtctggac gcgctcgagg acggatgagc
    15181 ggcgggcaaa cccgccgaaa ccggaagcga gcgagttcct tctcagggcg ttctcagcgg
    15241 agcggagttt acagatacac aacgggcaca cccggccgca ccgggcgcgt cctgcgcaga
    15301 cgccgctcgt tgaagaacgg agggccggca cccgccgccg gccctgcatc accaggtcga
    15361 cgaagacgga gaggaaacga tgacccagcg agagagctgg cccgccgaag ggtcgggcta
    15421 cgcgcccaag acgacggcgc cgcgcaagga gaacccggtc aagcgcttcg gcgccacgat
    15481 cggcttcctc gccgacgcat cacgtcgccg gccgggaggc cgccgggtgc tttgggtgct
    15541 cgtgtcgctg ctcgccgtca ccggcgtcgg aatgatgacc tacccgttcg caacgcagat
    15601 ctgggccaac cagatccaga aggacaaggc gaagcagttc gccagcagcg agctcctcaa
    15661 cgcctacaag accgataccg tcggcgcggc tgtcggtgag ggcgaggcgc tgactcgctt
    15721 gcagatcccc aagctgaagg tcgacgtgat cgtggtcgaa ggcgtctccg gcaacgcgct
    15781 gcgcgcgggt gccgggcatt acccggaatc cgcgcttccc ggcgacgtcg gcaacgtcgc
    15841 catcgccggg catcgaaccg gcttcggcca gccgttccgc gacctggacc ggctcaaggc
    15901 cggggacacg atcatcctga ccacgccgct cggcaattac acgtatcgag tgatgccccc
    15961 gttcgacggg catcccaacc cgtggatcac gcatccgctc gattggaccg tgatcgctcc
    16021 cacccccacc cctgtcctga cgctgaccac gtgcgatcca ccgcacacgt ccaagaaccg
    16081 cttgatcgtg cgggcggagc tggtggatac ccaggaaagc tgaggaacag tccccccgca
    16141 agaaaacgac gaggcccgcg acaccgatcg cgggcctctt cgtgtcttcg gtgtgatcag
    16201 gctccgaggt acgcctccat gacgcggcgg tcttcgagca gggtgattcc gctcccctcc
    16261 atggtgatct cgcccgtctc gagcacgtag ccgcggtccg cgatcgccag cgcctgaaag
    16321 gccatctgct ccaccagcag gatcgtgatt ccttcacgct taagctcctg gatgatctcg
    16381 aagatctgct tgacgatcag gggagcgagg cccatcgacg gctcgtcgaa gagcagcacc
    16441 cgaggacgcg ccatcagcgc gcgcgccatc gcgagcatct gctgctcgcc gcccgacatc
    16501 agccccgccg gctgggaacg ccgctcaccg aggatcggga agcgctcctc catgcggcgg
    16561 atgtcgcgtt cgacgtcggc atcgcggcgg ccgaacgcgc caagcatcag gttgtcgcgc
    16621 acggtctggt tcggaaagac cttgcggcct tcgggcgagt gagcgacgcc gagtcgcgcg
    16681 atcttgtgcg ccggccacgt ctccacgcgc cggcccatga tcgtgatgtg ccctcgtacg
    16741 gtcttgtgaa gctcgctgac cccggagacg gtcttgagcg tcgtggtctt gcccgctccg
    16801 ttcgagccga tgatggcgac ggtctcgccc tcgtggaccc ggaacgacac gccgcgcagc
    16861 gcctggaccg cgccgtagaa gacgtgcagg ttctccactt cgatcacgac gccgccgcgg
    16921 tcgcgcgcgg gtgccggccc ggcgggggcg acgcgaacgc cggagatcgg ctccggctcg
    16981 ttgatcgtga gcgtgtcatg cgtttcgtgc gccatgcgtt cctcgacgac cgcgcccggg
    17041 accgaggccg ccgcgagacg ggtcagggca gcctcgggct cctcggttcc gatcgcctct
    17101 cgtcgctcct tcgcggcgtc cctgcggacc tcgcgggacg cgagctggcg ctcggtcgtg
    17161 cgttgcgcgg ccgcgagcac cgcgtcggga atcgctcctt cgtgctcgat cgcgagctcg
    17221 ggcggcaggt cgtcgtcgcg ccggactcgc ggccgcgcgg cggcgggctt cttcgcaggg
    17281 ttcttccgcg cagcctgctt cttcttcggt gcgggcgtct tcgcggatgc cgcagtccgc
    17341 ggggtctttt tccccggggc ctttttcggg agggccttct tcgcggccgc tgctttcttc
    17401 ttcgcgggca tcgtcgctcc tagacgtggg cctcggcgcc gaggtacgcc tcgacgacgc
    17461 gctggtccgc ttggatctcg gcgggagtcc cgtcggcgat cttgctgccg tagtcgagca
    17521 cgctgattcg gtcgctcacg cccattacca gatccatatg gtgctcgatc aggaacacgg
    17581 tgatgccggt gcgctggatt cgccggatca ggtccatcag atcgtgaact tcggccgggt
    17641 tcaagcctgc ggccggctcg tccaggatca gcagcaccgg atcgagcgcg agcgcgcgcg
    17701 cgatctcgag gtatcgctgc ttgccgtacg gcagcgttcc ggcgagctgg tcggccacgt
    17761 cctccagccc cacgaactgg agcaggccca tcgcgcgggc cttgatgcga gcctcctcgc
    17821 gccgcgaccg gccggtgcga accatgttgc cgatgaaccc ggagcccacg cgcacgtgca
    17881 tgccgaccat gacgttctca aggacggtca tgcggcgcca cagctggagg ttctggaacg
    17941 tgcggcccat gccgcgctcg acggtgatga aggccggttg tccgtcgatg cgttcgcccg
    18001 cgaaatacac cgatccttcc tcgggtgcgt acacgcccgt gacgacgttg atgaatgtgg
    18061 tcttgcccga gccgttcggg ccgatgagcg aatggacggt gccgcgctcg acgcggatcg
    18121 ataccgaatc gttggcgcgc aagccgccga agatcttcga catttcgcgt gtctcgagca
    18181 ccacgtcccc ggtggcggcg tcctcgcgcg gcgtggggcg caccagcgag gccggcagcg
    18241 ccggcggccg cggcggcggg ttgcgatagc ggtggcgcgt gcggcgccgg tgaatgcggt
    18301 cgttgatgat cccgacggtt ccgcccggag cggcgacgac gctcaccagc gcgaggatcg
    18361 ccagcagggt ggtcgtccag cgcttgaggt cgaagtccag gatctcgccg accttcggga
    18421 tcagcaaccg gatcagcgac accagcgccg cgccgaccag cggcccggcc agcgtgccgg
    18481 tgccgccgat gatcatcatc acgacgtaga cgaacgagtc gcggatgtgg accgccgcgg
    18541 ccgactcggg cgagatctgc gcggtcgaga atgaggtgag gagggcgccg gcgagcgagg
    18601 cataagccgc cgagaccatg aacgcggcca ccttgacctt gtagacgcgc acgcccgagg
    18661 acttcgcggc gatctcggat tcgcgcacgg cggtcatggc ccggccccac cggctcttga
    18721 cgatgttgcg tgccaggaag aacaggagcc cgaagatcag caccaggacc aggtagtagt
    18781 cgacgtcggt catcgccgtg ccggtgaacg ggatgttgat ggttcggttc ggtggccggg
    18841 caatgttgcc gatcggggtg gcgatgtcgc cgatgccttg cgcgccgccg aagaccggct
    18901 tgagaagctc gctgttgaac agcgtgtaga agatcggcac gaacgccagc gtcacgatgg
    18961 acaggtacgc gcccttgacc cgcagggccg gcaagccgag catcgcaccc gccgccgctc
    19021 cgagcgcgat cgccacgacg agcgagatcc agtgattgcc gagcaggctc gagtcgaatc
    19081 gcgtcgtcat cacgcccacc gtgtagccgc cgatgagcac gaagatcttc tggcccagcg
    19141 aaagctggcc ggtgaacccg gcgatgaggt tgaggccgat gaccacgatt gtcgccgcca
    19201 ggatcttttg aacctccagg aacgtcgagg gatcgaagcc ggggagcgcg aacggccagg
    19261 caagcagcgc gatcagcagc agcgtgaacg cgatcccgcc cggggtcgcg aagacgctca
    19321 gtcgcggctc cgggcgcagc gccatctcgg ccgccgcctc gggcaagacc gacggcggcc
    19381 gcgcggcggc gggagcgccg gcggcccgcg cgcgcgtcca ggctttcagc gcgcgccggc
    19441 ggtcccgttc cgcgaccgcc gtgcggttcg cctctttgag ccggtcgagc atcgtcacgc
    19501 gcctacacct tctcgatcag ggcttcgccc agtatgccgg tcggcttcac gaccaggatc
    19561 acgatcaagg tcaagaacac cagcgccgag gtccactcgc ccgcgccggc ctgcttggcg
    19621 agcgactcga cgacgccgat cgtgagcccg cctaccagcg cgccgcgcgt cgagccgatc
    19681 ccgccgacga cggcggcgac gaacgcggtg aggcccacga gcgtgcccat gtcggccgag
    19741 acgaagatga tcggcccgag gagccatccg ccgacgccgg cgagcagccc ggcgagcgcg
    19801 aacgagtaac gaaccatgaa gccggtgttg atccccatca gactcgcggt cgccttgtcg
    19861 tgcgcggtag cgcgaagggc ccggccgagc tgcgtcttgg agtgcacgac ctcgagcacg
    19921 accgcgagcg ccaggcccac cacgagcact agatactgct tcggctcgat gctgaacacg
    19981 gtggactttc cgccgatcga gctgatcacc ggctcgacgc gcttggcctg ctcgccggcc
    20041 aggttccgca cgacctcgac gaagatcagc gacaccgaca aggtcgtcag gatccacccg
    20101 atgttggtgt tcggatcgaa cttgcccagc ggcgcgatcg agatcagatg cacgaccagc
    20161 ccgacgatcg tcgcgccgaa caacgcgatc accagcgcga gcggccacgg caacccgagc
    20221 gcgttgccgc cgctggagac cgcaaagaag tacaggtagg caccgatcat cagcacctcg
    20281 ccctgcgcga agttgatcac gcgggtcgtc atgtagatga tcgagaacgc cagtgcgatc
    20341 agcgcgtaca cgcccccggt cgtcagaccg agcacgatcg cctgcagaaa ctccaacgcc
    20401 cccccttagt ggatgtcgca ctcgttcgtc agacggaaac cccagtcgct gccgggatcc
    20461 ggccgcttcg tgcattgggt cccgaagcct tgcttgagca tcacgtccac gaacagtttc
    20521 cggtgctttc gtcgcaggcc gtcctggttg aagcgcatga acatcgtcgg tccgatgtaa
    20581 ccgggcgggt tgaacggctt gatgccgagg tcgtcgttgc cggtgaactc ggtgccgagc
    20641 gtgtagggcg gatcggccgg ggccgcccgc ccgcgctcga gcgtgatgcc caccatgtcg
    20701 tccttctcga gcgagatgtg ccggtctttc gagaacgtgt gcggcgcgat cgaggagaag
    20761 ttgatgccct tctccatgct ctcgagcgcc gcgatgacct tgtcgccgtc ggtggactgc
    20821 gcgtcctgga acgccttcgc gaacaggaac accgcgtcgg ccgggttgtt ctcgccgccc
    20881 gtgggcgagt cgccgaactc ttccttgaac agatccacgg ccggctggaa ctcggggatg
    20941 tagccgatcc cgcccatgta ccagtacgac atcgtgccca cgcgcgcggc gtcgccggcc
    21001 aagaccgcga acgtgcgctc ggccgcgccg ccctcgaagc cgatgatctg cggatgccat
    21061 cccgggccgc gcagcgcggt cgtccggttg agatagacgg cgtcgagtcg cttcaggctt
    21121 tcgagcgcgt gcgccgtgtc acccgcgatg ccccagatca tcagcacatg cgggttcgtc
    21181 gagcggatgc gctgcagctg cgcggtcatg tccacgtcgc cgacgttgta ctgctcggcc
    21241 gcgagcagcc ggtttccgct cttggcgagg gtctcgccga tcaggcgctt gccttgcgcg
    21301 ccgatcgcgg tgttgtcccc cagataggcg aagcggcggt agccgcggtc ggcgatcgag
    21361 tactcctcga gcgcgatctc gatgccccac ttgtcgggcg gaaacatctg gaacagctgc
    21421 cggtaatcgg tctgcggata gagctgcccg acgctggcga tgtcgccgta ggccgcgatg
    21481 gcgggcaagt tgtactgctg cagcagccgc ttggcggcgt agatgtcgtc gtagaggccg
    21541 gcgaagatca gccccttgat gttgaacgag gtgatcatct cttggatcgc ggagaccgcg
    21601 acctgtgccg aaaggttgtc gtcgcgcgtg atcagccgca gcttcacggt gcggtccccg
    21661 atcttgatgc cgccgaggtc ctgcttgcgg taggcctcga agtcgggaaa cccgttgcgc
    21721 gaggacatga tcgccgggtc cggcagcccg tggcggttga tctgcttcgt cgccagcccg
    21781 gcgccgcggt cgagcagctt gcccacgaac gcaccgacgc ccgagtagat cgagatcgcc
    21841 ccgatgttga tcgtgtcgcc gtccgcgccg ccgcccacgt cgaacttcgc gccgcgcgag
    21901 ggcgacagcg acgaggcggt gcggccgcag gcggccagga aggcggggaa cgcggccgag
    21961 gcggccgccg ccccgaggcc gaggcgcttg gacaggtcca ggaacgcgcg gcggttgatc
    22021 ggcgtgtccc agccgagctt cgagcgctct tccatctcgc gttcccatcg cgccgcctcg
    22081 cgccggttgc gcggcgtgcg cgccggctgc tcgtaggacg ggaccggctc gcccggccgg
    22141 ggcggcgccg gctcggcggg ccgcgcgtcc cactcgtcgg cgtggcgagg ctcgcgccgg
    22201 gtcacggcga tccctccggc ggcggggcgg gagccggcgc cccgggcatc aggtctcgcg
    22261 gcggtgcgag gcgctcgatc tgcggaacca agatcggcaa gcccgccaga tccggcagct
    22321 cgccggcccg gtaagggccg gtgccgcggc ccatcagcgt cacgaccgtc cacgccagcg
    22381 cggtcatcac cacgagcatc aacgccacgc gcgtgcgcac gaaggcgtcg gccatgcgat
    22441 gcgtcatcgg gcggctcccg cggattcggc gcgcaccacg atgcgatcgc tgcttcccca
    22501 cgcgcgcgcc ggcgcggcga gcagcagcac cggcgtgacg gagtcttcgc gcggatcgat
    22561 cgccgatgcg ggaagcactc ggacgctgcg aacggtgaac cggcgcacgc cggcgagcgt
    22621 ctcgaccacg atcgtgtcgc cgggaatcag acggtccaga tgcaggaaca tgttgccgta
    22681 gccgaaacgg ccggcctcga tcacgctcac gccgggcagg cccgggtacg cgctgccggc
    22741 cgcgagcgcc ggcccttgcc gcgcgccgtt cccgccggcc accacgatca ggtcggcgcc
    22801 ggtggcggcg atcccgagtc gcgccaccgg ctcgccgggc gtgaacgcgc gggccgcgag
    22861 cgtcgcgggc gcgaccgggc cggccgcgac cgccggctcc cactgggcgc gcagcgaccg
    22921 ctgcgcgagc gaggcacgca cgttcgtgga cacgtacagc ccggacagca gcgccggaac
    22981 gagcgtggcg agtgcgatcg cctgccatcg gtgcagcacg agccggggcg cggcccctgc
    23041 cgcggccggc gctcgcgccg cgaggttcat cggcccacct cgcgtacctc gacggagata
    23101 tcgccgcggc ggcgtagcca ccaaaacatc agcaggttgc cggtcagcag cgcggtcgcg
    23161 cccacgctcg cgatcccgcc gggaaggcgg aacccgggcg gtgccacctg gttcttgtac
    23221 caatcctgcg gcgtccagac gcggtcgaac gtgccgagcg c
//
LOCUS       DATLFO010000049         4831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_60508, whole genome shotgun sequence.
ACCESSION   DATLFO010000049 DATLFO010000000
VERSION     DATLFO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60508"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VM841_01735"
     CDS             <1..399
                     /locus_tag="VM841_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HVL88934.1"
                     /translation="REVADRIVAKPGTEPYGAVSAKIAYLAEASVVFKVSRRVFLPEP
                     AVESVVLSIRRRAKAPVAGIKDRIFGVIDAGFAQRRKTIRAALRNAGVDPSRIEEALE
                     IAGIAGETRAERLGLPQFAAIASVLRVPRR"
     gene            396..1268
                     /locus_tag="VM841_01740"
     CDS             396..1268
                     /locus_tag="VM841_01740"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HVL88935.1"
                     /translation="MKVLAPAKINLWLSVGARRPDGFHEIESVMQTVSVFDSLEISAR
                     EGIAFDVMPPGAAPEDESNLVIKSVRALWGVLGRTPGAALTLSKTIPVAAGLAGGSAD
                     AAAALVGLNELWDGRLSRKALEKVGAAIGSDVPFCVRGGTCAVRGRGEVLSPLPAHDP
                     LWWVIAVPDASLSTVQVYAEFDRLNALPALEDPFELADALARGDVDRIGGLLRNDLQD
                     AAVSLLPSLAAGGDVLRSAGAAGAIVSGSGPAWIGLCRDEAHAGSVADAVRDRFAFVA
                     AATATGRGPVVENP"
     gene            1346..2065
                     /locus_tag="VM841_01745"
     CDS             1346..2065
                     /locus_tag="VM841_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88936.1"
                     /translation="MTAEGTRERAIELRRAGATHKQIAVELNVSMGLLCRWIKDAGLV
                     GAGRTAQMDRDRAVELRRMGQSYSEIERETGVAKSTLSYRLKDIPLTPEHAALLAQKR
                     LQGAVLLYWAEGSKSKPWSRGHRVKLSNSDPGMVRFFLRWLTVLGVGVDQLVLRVQIH
                     ERADIDAAHRIWSQVTGVPVELFRRPTLKRHNPKTTRRNVGAGYIGCLSVEVTGSTEL
                     YLRIAGWYEGITAKLTESLAP"
     gene            2056..2126
                     /locus_tag="VM841_01750"
     tRNA            2056..2126
                     /locus_tag="VM841_01750"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2088..2090,aa:Gln,seq:ttg)
     gene            2164..3597
                     /gene="glmU"
                     /locus_tag="VM841_01755"
     CDS             2164..3597
                     /gene="glmU"
                     /locus_tag="VM841_01755"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HVL88937.1"
                     /translation="MSKRNSSGIRERAVALIVLAAGESKRFKSRTPKVLHDLCGLPVI
                     AHVLGRLAGLGAARTVLVVGREADSVKAAAKAVDPKIRFAEQTKRLGTADAARVGDEA
                     LGAFKGDVLVTPGDTPLLDPATMRDLLDRHRATGAAATVLSAHFDDPTGYGRIVRDPD
                     GGVARIVEHADATEAERVITECNAGVYVFDRAALRSALTKVEAANRQGELYLTDVIEI
                     LRDKGERIEAHLAPDARQTMGINDRAQLAEAAAVIRAQIAHDLMRGGVTIEDPATTYI
                     DATVTAGPDTIIRPMTFLRGATTIGRGCVIGPEAELVDCTIGHDVTISRAVARETTLE
                     TGASVGPYASLRPGTVLKPGAKAGTFVEMKKTTVGKGSKVPHLSYMGDATIGSGSNIG
                     AGTITCNYDGETKTKSATIIGDGVLVGSDTMLVAPVTLAKGAVTGAGSVVTHDVPGHT
                     VVAGVPAKPRRKRKPAPKDSKKKGNGK"
     gene            3594..4583
                     /locus_tag="VM841_01760"
     CDS             3594..4583
                     /locus_tag="VM841_01760"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HVL88938.1"
                     /translation="MSELEVSTKKRLVLVAGRSNPELAGEIARNLGVDLGEVEIEDFA
                     NGEIYVRFASNVRGADLFVFQTHSPSGAHDSINSAIMEQLIMIDAAKRASAKRISAVI
                     PFYGYARQDKKSRGREPISARLVADLLQAAGADRILTVDLHTAQIQGFFDRPVDHLTS
                     LPILADYLADRFDGDLVVVSPDAGRVRVAEKFAARLNTSLAILHKRRRTDIRNVSETL
                     EVVGEVAGRRCVLVDDMIDTAGTICGGAEALMKFGAEEVLALATHGILSDPATDRIKN
                     APLKELVVTNTIPLPAEKQIDKITVLSIAPVIADTIRAVFADESVSEIFHGEN"
BASE COUNT          788 a         1628 c         1666 g          749 t
ORIGIN      
        1 cgcgaggtgg ccgaccgcat cgtggccaaa cccggcaccg agccctacgg cgccgtctcc
       61 gccaagatcg cgtatctggc cgaggcgtcg gtggtgttca aggtctcgcg acgcgtcttc
      121 ctgccggagc ccgcggtcga gtcggtagtg ctctcgatcc gcaggcgtgc gaaggcaccg
      181 gtcgccggga tcaaagaccg gatcttcggc gtcatcgacg cggggttcgc gcagcgccga
      241 aagaccatcc gcgccgcgct tcgcaacgcg ggcgtcgatc cgtcacggat cgaggaggcg
      301 ctcgagatcg ccggcatcgc cggggagacg cgcgccgagc ggctcggcct gccgcagttc
      361 gctgccatcg cctccgtgct gcgggtgccg cgccgatgaa ggttctggcg ccggcgaaga
      421 tcaacctgtg gctctcggtc ggcgcgcgcc ggcccgacgg gtttcacgag atcgagtcgg
      481 tgatgcagac cgtgtcggtg ttcgactcgc tagagatatc ggcgcgcgag ggcatcgcct
      541 tcgacgtgat gccgccgggc gcggcacccg aggacgagtc gaacctggtg atcaagtcgg
      601 tgcgggccct atggggggtg ctgggtcgca caccgggggc cgcgctgacg ctgtccaaga
      661 cgattccggt cgcggccggg ctcgccggcg gctcggccga tgcggccgct gctctcgtcg
      721 ggctcaacga gctatgggac ggccggctgt cgcgcaaggc gctcgagaag gtcggcgcgg
      781 cgatcggcag cgacgtgccg ttctgcgtgc gcggcggcac ctgtgcggtg cgcggccgcg
      841 gagaggtcct gtcgccgctg cccgctcacg atccgctctg gtgggtgatc gcggtcccgg
      901 acgcgtcgct gtccacggtg caggtgtatg ccgagttcga tcgcctcaat gccttgcccg
      961 cgctcgaaga tccgttcgag ctcgccgatg cgctggcgcg cggcgacgtc gatcggatcg
     1021 gcgggctgct gcgcaacgat ctgcaggacg ccgccgtgtc gctgctgccg tcgctcgcgg
     1081 cgggcgggga cgtgcttcgt tccgcgggag cggccggtgc gatcgtgagc ggcagcgggc
     1141 cggcatggat cggcctgtgc cgcgacgaag cgcacgcggg atcggtcgcc gatgccgttc
     1201 gcgaccggtt cgcgttcgtc gcggccgcca ccgcgaccgg tcgcggtccg gtggtggaaa
     1261 acccctagat tcccagaggt ttctgtcgta ggcaccgcat agcatggagt cgtgccttcc
     1321 ccgtttctcg gcgagaggag acggcatgac cgcggaaggc acgcgcgaac gcgccatcga
     1381 gctgcgacgc gccggagcga cccacaagca gatcgctgta gagctgaatg tgtcgatggg
     1441 cctgctctgc cggtggatca aggacgccgg gctggtcggc gcaggtcgca ccgctcagat
     1501 ggatcgagat cgggcggtcg agctccgaag gatgggacag tcctattctg aaatcgagcg
     1561 agagacaggg gttgcgaaga gcaccttgtc ctatcggttg aaggacatcc cgctcacgcc
     1621 tgagcacgcg gcgctgctgg cgcagaagag gcttcagggc gcggtcctgc tctactgggc
     1681 cgagggctcc aaatcaaagc cctggagccg agggcatcgc gtcaagctct ccaactccga
     1741 tccgggcatg gtccggttct tccttcgctg gctcacggtc ctgggcgtgg gtgtggacca
     1801 attggttctt cgggtccaga ttcacgagcg agcggatatc gacgcggcgc accgcatctg
     1861 gtcgcaggtg actggcgtcc ctgtagaatt gttccgtcga ccgacgttga agcggcacaa
     1921 cccgaaaacc acccggcgaa acgtgggagc gggctatatc ggatgcttgt ccgtcgaggt
     1981 gaccggcagc accgagctct acctgaggat cgctggttgg tatgaaggaa taaccgcaaa
     2041 gttgactgaa tctcttgccc cgtagtgtaa aggcagcaca gtagaatttg gctctactag
     2101 tgggggttcg acccctcccg gggcagcgaa tgtgctagtg gaatgataac ccaaggggat
     2161 caagtgagca agcgcaattc gagcggcatc cgcgagcggg ccgtcgcgtt gatcgtcctc
     2221 gcggcgggcg aatccaagcg attcaagtcc cgcaccccga aggtgctcca cgacctgtgc
     2281 ggcctgccgg tcatcgcaca cgtgctcggc cggctcgcgg gcctcggtgc ggcccggacc
     2341 gtgctcgtgg tcggccgtga ggcggacagc gtcaaggccg ccgccaaggc cgtcgatccc
     2401 aagattcgct tcgcggagca gacgaagcgg ctcggcacgg ccgatgccgc tcgcgtcggc
     2461 gacgaggcgc tcggcgcgtt caaaggcgac gtgctcgtca cgcccggcga taccccgctg
     2521 ctcgaccccg ccacgatgcg tgacctgctc gaccggcatc gcgcgaccgg cgcggccgcg
     2581 acggtgctca gcgcgcactt cgacgatccc actggctacg gccgcatcgt gcgcgatccc
     2641 gacggcggcg tcgcccgcat cgtcgagcac gcggacgcga ccgaagccga gcgggtcatc
     2701 accgagtgca acgcgggcgt ctacgttttc gaccgcgccg cgcttcgctc ggcgctcacc
     2761 aaggtcgagg ccgccaaccg tcagggcgag ctgtacctga ccgacgtgat cgagatcctg
     2821 cgcgacaagg gcgagcgcat cgaggcgcac ctcgcgcccg acgcgcgcca gacgatgggc
     2881 atcaacgacc gcgcgcagct cgcggaggcg gcggcggtga tccgcgcgca gatcgcccac
     2941 gatctgatgc gcggcggcgt caccatcgaa gatcccgcca ccacctatat cgacgcgacg
     3001 gtcacggccg gccccgacac catcatccgg cccatgacgt tcctgcgcgg cgcgaccacg
     3061 atcggccgcg gctgcgtgat cggccccgag gccgagctgg tcgactgcac gatcggccac
     3121 gacgtcacga tcagccgggc ggtcgcgcgc gagaccaccc tcgagaccgg cgcctcggtc
     3181 ggcccgtacg cgagcctgcg gccgggcacc gtgctcaagc ccggcgccaa ggccggcacc
     3241 ttcgtcgaga tgaagaagac gacggtcggc aaggggagca aggtgcccca cctgtcctat
     3301 atgggcgacg caaccatcgg ttcgggctcg aacatcggcg ccggcaccat cacctgcaac
     3361 tacgacggcg aaaccaagac gaagtcggcg acgatcatcg gcgacggggt gctggtcggg
     3421 tccgatacca tgctggtggc gccggtgacg ctggccaagg gagcggtcac gggcgccgga
     3481 tcggtcgtga cccacgacgt cccgggccat accgtcgtgg cgggcgttcc ggccaagccg
     3541 cgccgcaagc gcaagcccgc accgaaggac tcgaagaaaa aggggaacgg gaagtgagcg
     3601 aactcgaggt ctcgacgaag aagcggctgg ttctggtcgc aggacgctcc aaccccgagc
     3661 tcgcgggaga gatcgcacgc aacctgggcg tggacctggg cgaggtcgag atcgaggatt
     3721 tcgccaacgg cgagatctac gtgcgtttcg cctccaacgt ccgcggcgcc gacctgttcg
     3781 tgtttcagac ccacagccct tcgggcgcgc acgactccat caacagcgcg atcatggagc
     3841 agctcatcat gatcgacgcc gccaagcgcg cgagcgcgaa gcgcatcagc gcggtcatcc
     3901 cgttctacgg ctacgcgcgg caggacaaga agtcgcgcgg ccgcgagccg atctcggccc
     3961 gcctggtcgc cgacctgctg caggcggccg gcgccgaccg catcctgacg gtggacctgc
     4021 acaccgcgca gatccaagga ttcttcgacc gtcccgtcga tcacctgacc tcactgccga
     4081 tcctggccga ctacctcgcc gaccgcttcg acggcgatct ggtcgtggtc agcccggacg
     4141 cgggccgcgt tcgcgtggcc gagaagtttg ccgcgcgcct taacacctcg ctcgcgattc
     4201 tgcacaagcg ccgccgcacc gacatccgca acgtctccga gaccctcgag gtcgtcggtg
     4261 aggtcgccgg ccgccgctgc gtgctcgtgg acgacatgat cgacaccgcc ggcacgatct
     4321 gcggcggtgc cgaagcgctg atgaagttcg gggccgagga ggttctggcg ctcgcgacgc
     4381 acggcatcct ctcggatccc gcgaccgacc ggatcaagaa cgcgccgctc aaggagcttg
     4441 tcgtcacgaa cacgatcccg ctgcccgccg agaagcagat cgacaagatc acggtccttt
     4501 cgatcgcgcc ggtcatcgcc gacacgattc gcgccgtctt cgccgacgag tccgtcagcg
     4561 agatcttcca cggcgagaac tagctgccga agctgccgaa gccggtccgg ccggatccgg
     4621 acattgggtc gatccggtaa tgaccgaagg atccgggggc gccacgcaga attgccctcg
     4681 ggatgaaacc cacccgcagc ctcgtcctgg tgttcgcttt gggtgtcgcc tcgctcgtcg
     4741 cgtcgttgcc tgccgcggtg gccatgccgg cggccggcgt gccggtgaat tcgcgggagt
     4801 ttctcctcgc gtcggtcagc acgaccgctc a
//
LOCUS       DATLFO010000050        10776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_22008, whole genome shotgun sequence.
ACCESSION   DATLFO010000050 DATLFO010000000
VERSION     DATLFO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22008"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VM841_01765"
     CDS             <1..1325
                     /locus_tag="VM841_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HVL88939.1"
                     /translation="GQQASAGGFTGAVLLGMGGSSLAPETMRRILGVAGGAIDLRVLD
                     STHPQAVRRIEREMDLRRTLFIVASKSGSTIETLSHLQYFYGRIQRGTHFVAITDPGS
                     ALEALAGKHDFAGLFLNPEDIGGRFSALSLFGLVPAAVIGADLDALAAGAITMAQRCR
                     AAAADNPGTVLGAALGEAALAGREKASFVYPEAMAALGAWVEQLIAESTGKDGRGILP
                     VAGEAPGEPGVFGDDRFFVDTGGHDWVGATGAPRLVLEADGGSPAERLGGELEAEEFA
                     TAVAGHVLGIQPFDQPDVASAKKATSEILEAGGAAAPQFEDPSAVLDPPPSYLAILAF
                     VDPSQENAAVLERVRTALRDRLGIAVTAAFGPRYLHSTGQLHKGGPAGGGYLIVTDEM
                     HAEDVAIPGRGYTFGTLIDAQAEGDLRSLRDAGRRVARVRMRDLEPLA"
     gene            1357..2166
                     /locus_tag="VM841_01770"
     CDS             1357..2166
                     /locus_tag="VM841_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL88940.1"
                     /translation="MKPLTGKVVLITGASRGLGVDMARAFAGKGARLALAARSAEDLE
                     SVRKELGSEAIAVVADLRDLGSLRSLVTTVESSLGPVDVLVNNAGLEQVCDFESMDLD
                     QMKAIIDTNVTGLLWLSRLIVPSMVARRSGHIVNIASLAGLNPVPHNTVYSASKHAVV
                     GFSLSLRAELEEHGVEVSVVCPSFVTAGMFAEWGRPAPKAVGAVDSPSVAKGVIEAVE
                     KNKPLVTVAKGLGKIGHASFALAPGLALGTMKKTGVVRYMREQAEINAKRG"
     gene            complement(2163..2687)
                     /locus_tag="VM841_01775"
     CDS             complement(2163..2687)
                     /locus_tag="VM841_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88941.1"
                     /translation="MTLLGAAHSLASNPSAPDAHRLAESAARNAALLARLVQDLQRIA
                     HRGTARPRREMIEVMPALRASIGAGNVRIDGDEKLIAIAALSDLTAFIDALAAAPGGR
                     THWVHAREEGPAIQISFRPAFPDRPLDAGLIRSLAAPLGPVAIGVDGSVTLSIPALNQ
                     EPAAVDAGARWRAN"
     gene            2982..4946
                     /gene="acs"
                     /locus_tag="VM841_01780"
     CDS             2982..4946
                     /gene="acs"
                     /locus_tag="VM841_01780"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HVL88942.1"
                     /translation="MTDEKNIDALLEENRIIEPPAWFRERALVADDEIYGRAAKDPEA
                     FWAEQAERLAWFAPWKTVMTWTPPWVRWFEGGTLNATVSCLDRHSDATPDRVAYHFEG
                     EPGDRRAITYRELTEDVCRFANALRSLGVGKGDRVAIYMGMVPELPMAMLACARIGAV
                     HSVVFGGFSPEALRDRIHDAQAKAVITCDGAWRRGQIVPLKANVDAAVEQSPSIESVV
                     VVRRIGDQADAAMKAGRDHWYHELVESAAPECEPERMDAEDPLYILYTSGTTGKPKGI
                     LHTTGGYMTGAAATHRLVFDIHPETDVYWCAADCGWVTGHSYIVYGPLANGTTGVLYE
                     GAPDFPDKDRWWEIIERYKVTILYTAPTAIRSFMKWGTDYPARHDLSSLRLLGSVGEP
                     INPEAWIWYWTHIGGERCPVVDTWWQTETGAIMITPLPGVTPLKPGSATRAFPGIEAD
                     VVDDKGQSVPAGAGGYLVLRKPWPSIARTVYGDDDRYVQTYFGRFGPETYFAGDGAKR
                     DEDGCLWLLGRIDDVMNVAGHRLSTFEIESALVDHSAVAEAAVVSRPDDVVGEAIVAF
                     VTLKGTEHGDEKLIAELREHVAAKIGKIARPHSIVLTDDLPKTRSGKIMRRLLRDVAG
                     GRPLGDATTLANAEVVEQIRRLAAERPTEE"
     gene            4948..5808
                     /locus_tag="VM841_01785"
     CDS             4948..5808
                     /locus_tag="VM841_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88943.1"
                     /translation="MSNLEQARRLLQALEPVAAAHWFAPDWREEMAAAGIDDYAPAYF
                     CSRAAPLGPVPWQVVHGLFYNWNPALVKMAVRWDVCAPADAWAANLRGVNRALTRVLA
                     AVDGAEIAKAAGMLREVATGCPQEGRGLYASYAALPWPDAPVEALWQAATALREFRGD
                     THVAVLVSHGLGPVEALVLNAAWIGADLQRYAGTRMWSEEDLKETVVALQDRAFLDAS
                     GAITDGGGKFREMIEMDTDRRALAPVEMLGAAAEDVLAVLEPIGKAVLGTPKAVPSII
                     GKVGRPATAG"
     gene            complement(5805..6467)
                     /locus_tag="VM841_01790"
     CDS             complement(5805..6467)
                     /locus_tag="VM841_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HVL88944.1"
                     /translation="MTDLAGAFPPIPPGCGPVALLSSNEFSPAADEFDHAMLAASRGA
                     RVALIVAAAGADAPNAARHGIDHYRRFGADPFVVPVYERGHAERGMLGELDVLFLCGG
                     SPRTLLRALRGSPLWDEAIERWQGGATLAGSSAGAMALCAHCTLPVPGARVPTRWSDG
                     LGPLSGVAVAVHADERPREWLEQIAATASWPVVAMESDTGVILRAGEAPAIAGPRRVR
                     VV"
     gene            complement(6477..8009)
                     /locus_tag="VM841_01795"
     CDS             complement(6477..8009)
                     /locus_tag="VM841_01795"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVL88945.1"
                     /translation="MNRSSGTDTPARGDLNLLVGDRYRLIEQIRGGGMGTVWKARDER
                     LGRLVAVKILHGTLDGDETARERFRREAIAASKVSHPAVASIYDYVEDTDRRAIVMEL
                     VEGETLAQRIARGPLSPDEARRIFAQVLDGVQAAHDCGVLHRDIKPSNILLTAGGTVK
                     VADFGVATMTGESTLTVTGAIMATPQYAAPEQLRGAGSTPETDVYQTGVALYETLTGH
                     LPFTGDTPVTVAMARLSQDPAPISMPEALPLSSVAMKALERDPAGRFATAAEMRAALL
                     DETLPAVVPLPADATMRLDSSDPGTVVLGAAAAPAGAAAIPNAGRPHRLRRVALRAAL
                     LALILALVATGLTAALSRGGSFAVPSLTNMTVDQASAKATQLGLEVAVETRPRPGLRE
                     GLVFSQDPGPGTSVKPGATLRLIAADGCCTVPALATYDGAKKTLEDAGLRMGKVTFVF
                     TVDAPPGTVLSQRPAADEAVAAGTPVDMVVARAPAPPPRERNQDQDQDKGKGKGKDKD
                     DD"
     gene            8075..9163
                     /locus_tag="VM841_01800"
     CDS             8075..9163
                     /locus_tag="VM841_01800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVL88946.1"
                     /translation="MITLKSRLIAHIRANGPVSFAEYMTAALYDPDDGFYARPPVGAA
                     GDFVTSPHVSAGFGLLLSRQIEGMWRAAGEPADYVVTEVGAGDGTLAAQIRGAAAGGF
                     AAALRYRGVEVSAGARAALEQAGIEAAASLTELAPAAAGCILAHELLDNLPFHRLRAR
                     GDDVVEVFVAERDGALIEIEGPPSADAVAALRWPMLDGEERPVSPAALRFLHAATGML
                     ERGHVLIYDYGFGEGERSQPVRAYEGQRVHDRLYDDPGSADITAGVDFGALVAEARSL
                     GLSVRGPAMQRDVLKALGFDDWFDDLRARRRQAEADRDSRAILRLLSEQSRAPLLTDP
                     AHLGAHLVLAAGIGDAGSPPGFGEGGEA"
     gene            9173..>10776
                     /gene="ligD"
                     /locus_tag="VM841_01805"
     CDS             9173..>10776
                     /gene="ligD"
                     /locus_tag="VM841_01805"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVL88947.1"
                     /translation="MPKKERLAPEPFTVATPVPLEAEKRGDAWWTVVAGRELRLSNLR
                     KIFWPEQGYTKGDLIAYYHAIAPMILPYLSERPLTMKRMPDGIDGPFFYEKSAPSHVP
                     DWVPRCPIPSDEGGVTDYMIAADEACLLFVANLGAIEFHPLHSRCGYDDRPDYLFFDL
                     DPMETGFDDVRVVARHVRAALDALGLPSYPKTSGATGMQIYVPIKLGPTHEQTRAFVG
                     AIGRMILKADPDRVTMEWEISKRTGKVFIDHNMNRPGANIAAVYSVRPEPGATVSTPV
                     TWDEVEAGVHPRDFTIVTIYDRLVATGDLFEGLLAEPIDLRPALERLGLPSEIDAELP
                     LREPPKPRVSVEQGTPKDRRLAEYIKKRDLAVTPEPGPGAAGAARESGDIFVIHKHNA
                     TRLHYDLRLEHDGALESWAVPKGLPTVPGDRRLAVHTEPHPIEYADFSGWIPEGHYGA
                     GESLIFDRGTIDKLEWTPGKVTVRLHGERVRGEYHLVKTSQGWLVFLSKDSAAEQPDP
                     PPQLAPMLATASKEPFDDPKWRFEPK"
BASE COUNT         1610 a         3719 c         3865 g         1582 t
ORIGIN      
        1 tcgggcagca ggcctcggcc ggcgggttca ccggcgccgt gctgctgggc atgggcggct
       61 cgtcgctcgc gcccgagacg atgcgccgca tcctgggcgt cgccggcggc gcgatcgacc
      121 tgcgcgtgct cgactcgacc cacccgcagg cggtgcggcg tatcgagcgc gagatggatc
      181 tgcggcgcac cctgttcatc gtcgccagca aatcgggatc cacgatcgag acgctcagcc
      241 atctgcagta cttctacggc cgcatccagc gcggcacgca tttcgtcgcg atcaccgatc
      301 ccggctccgc gctcgaagcg ctcgcgggca agcacgattt cgccgggctg tttctcaatc
      361 ccgaagacat cggcgggcgc ttcagcgcct tgtccttgtt cggcctggtg ccggcggccg
      421 tgatcggcgc ggacctcgac gcgctcgccg caggagcgat cacgatggcg cagcgatgtc
      481 gcgccgcagc ggcggacaat cccggcaccg tgctcggcgc cgcgctcggc gaagccgcac
      541 tcgctggccg cgagaaggca tcgttcgtgt atccggaggc gatggcggcg ctcggcgcgt
      601 gggtcgagca gctgatcgcc gagtcgaccg gcaaggacgg ccgcggcatc ctgccggtcg
      661 ccggggaggc gcccggcgag ccgggggtgt tcggcgacga ccggttcttc gtcgataccg
      721 gcgggcacga ttgggtgggc gcgacgggcg cgccgcgcct ggtgctcgag gccgacggcg
      781 ggtcgccggc ggagcggctc ggcggcgaac tcgaggcgga agagttcgcc accgccgtcg
      841 cgggccacgt gctcggcatc cagccgttcg accaacccga cgtcgcgtcg gccaagaaag
      901 ccacgagcga gattcttgag gccgggggtg ccgccgcgcc gcagttcgag gatccgtccg
      961 ctgtgctgga tccgccgccg tcgtacctgg cgatcctggc gttcgtggat ccgagccagg
     1021 agaacgccgc ggtgctcgag cgggtgcgca cggcgctgcg cgaccggctc ggcatcgccg
     1081 tcacggccgc cttcggaccc aggtacctgc attcgaccgg acagttgcac aaaggcgggc
     1141 ccgccggtgg cggttatctg atcgtcaccg acgagatgca cgccgaggac gtcgccatcc
     1201 cgggccgtgg ctacacgttc ggcacgctca tcgacgccca ggctgaaggc gacctgcggt
     1261 cgctgcggga cgccggccgg cgcgtggcgc gggttcggat gcgcgatctg gagccgctcg
     1321 cgtaggcttg cctcgtcacc gacgtgaggg ggcaccatga agccgcttac cggcaaggtc
     1381 gttctgatca ccggcgcatc ccggggcctg ggagtggaca tggcccgcgc cttcgccggc
     1441 aagggcgccc ggctcgcgct cgcggcacgc agcgccgagg acctcgagtc ggtgcgcaaa
     1501 gagctgggct ccgaggcgat cgcggtcgtg gcggacctgc gcgacctggg ctccctgcga
     1561 tcgctggtga ccaccgtcga gtcgtcgctg ggaccggtgg acgtgctcgt caacaacgcc
     1621 ggcctcgagc aggtctgcga cttcgagtcg atggacctcg atcagatgaa ggccatcatc
     1681 gacaccaacg tgaccggcct gctgtggctg agccgtctga tcgtgccgtc gatggtggcg
     1741 cggcgcagcg gccacatcgt caacatcgcc tcgctcgcgg ggctgaaccc agtgccgcac
     1801 aacaccgtct actcggccag caagcatgcc gtggtcggct tcagcctctc gcttcgcgcc
     1861 gagctcgagg aacacggcgt cgaggtctcg gtcgtgtgtc cgagctttgt gaccgcgggc
     1921 atgttcgccg agtggggccg gccggcgccg aaggcggtcg gcgcggtgga ctcgccgtcg
     1981 gtcgccaagg gcgtcatcga ggcggtcgag aagaacaagc cgctggtgac cgtcgccaag
     2041 ggtctgggca agatcgggca cgcttcgttc gcgctggcgc cggggctggc gctcggcacg
     2101 atgaagaaga ccggcgttgt gcggtacatg cgcgagcagg ccgagatcaa cgcaaagcga
     2161 ggctagttcg cgcgccagcg ggcaccggcg tcgaccgcgg ccggctcttg gttgagcgcg
     2221 gggatcgaca gcgtcaccga gccgtcgacg ccgatcgcga ccgggccgag cggcgccgcg
     2281 agcgagcgga tcaatccggc gtcgagcggg cggtccggga aggccggccg gaagctgatc
     2341 tggatcgccg gtccctcttc gcgcgcgtgc acccaatgcg tgcgtccgcc cggcgcggcg
     2401 gcgagggcgt cgatgaaggc ggtgagatcc gacaacgccg cgatcgcgat cagcttctcg
     2461 tccccgtcga tgcgcacgtt tccggcgccg atcgatgcac gcagcgcggg cattacctcg
     2521 atcatctcgc gacgcgggcg cgcggtaccg cggtgagcga tgcgctgcag gtcctgaacg
     2581 agccgtgcga gcaaggcggc gttgcgcgcc gccgactcgg ccaggcgatg tgcgtccggt
     2641 gcggatggat tgcttgccaa ggaatgcgct gccccgagaa gcgtcatcag cggctcgtcg
     2701 gccgtccgaa cgaaatggtc tgccgccagc gcccacgtcc ggctatgcga atgaatcaac
     2761 ccagtacccc cgattcgtga tcatgccggg ggaaagcatg ccgtccgcgg cctctggtca
     2821 tcaatggccc gattcggcgg tttgatgaga tcccccgaaa gaactaaccc gcccgggcgg
     2881 tgccgcggac cgcggacgaa cctcgatctc gatcgccgga cgatcgggcg aaccgccccg
     2941 cgatagagtt gccggtggga ccggaaaagg gagggggcgc cgtgaccgac gagaagaata
     3001 tcgacgcgct gctcgaggag aaccggatca tcgagccgcc ggcgtggttt cgtgagcgcg
     3061 cgctcgtcgc cgacgacgag atctacgggc gcgcggcgaa ggatcccgaa gcgttctggg
     3121 ccgagcaggc cgagcggctc gcgtggttcg ccccttggaa gaccgtgatg acctggacgc
     3181 cgccgtgggt tcgctggttc gaaggcggca cgctcaacgc cacggtgagc tgtctggacc
     3241 ggcattccga cgccacgccg gatcgcgtgg cctatcactt cgagggcgag ccgggtgacc
     3301 ggcgggcgat cacctatcgc gagctcaccg aggacgtgtg ccggttcgcg aacgcgttgc
     3361 gatccctcgg cgtcggcaag ggcgaccgcg tcgcgatcta catgggcatg gtgcccgagc
     3421 ttccgatggc gatgctcgcg tgcgcgcgga tcggcgcggt gcactcggtc gtgttcggcg
     3481 gcttctcgcc cgaggcgctt cgcgaccgga tccacgacgc gcaggccaaa gccgtcatca
     3541 cctgcgacgg cgcatggcga cggggccaga tcgttccgct caaggccaac gtggacgccg
     3601 ccgtcgagca gtcgccttcg atcgagtcgg tggtcgtggt gcgccgcatc ggcgaccaag
     3661 cggacgccgc gatgaaagcc ggccgcgacc actggtacca cgagctggtc gagagcgccg
     3721 cgcccgagtg cgagcccgag cgcatggacg ccgaagatcc gctctacatc ctctacacct
     3781 cgggcaccac cggaaagccc aagggcatcc tgcacaccac cggcgggtac atgaccggcg
     3841 cggcggccac gcatcggttg gtgttcgaca tccatcccga gaccgacgtg tactggtgcg
     3901 cggccgactg cggctgggtc accgggcact cttacatcgt ctacgggccg ctcgcgaacg
     3961 gcaccacggg cgttctctac gaaggcgcgc cggacttccc ggacaaggac cgctggtggg
     4021 agatcatcga gcgctacaag gtcacgatcc tgtacaccgc gccgacggcg atccgctcgt
     4081 tcatgaagtg gggcaccgac tatccggcgc gccacgacct gtcgtcgctg cggctgctcg
     4141 gaagcgtggg cgagccgatc aaccccgagg cgtggatctg gtactggacg cacatcggcg
     4201 gcgagcgttg cccggtcgtg gacacgtggt ggcagaccga gacgggcgcg atcatgatca
     4261 cgccgctgcc gggtgtcacg ccgctgaagc ccggatcggc gacgcgggcg ttccccggga
     4321 tcgaggccga cgtcgtagac gacaaaggcc agagcgttcc cgcgggcgcg ggcggctatc
     4381 tggtgcttcg caagccgtgg ccttcgatcg cgcggaccgt ctacggcgac gacgaccggt
     4441 acgtgcaaac ctacttcggc cgcttcggtc ccgagaccta ctttgcgggc gacggcgcca
     4501 agcgcgacga ggacggctgc ctgtggctgc tcgggcgcat cgacgacgtc atgaacgtgg
     4561 ccggccaccg cctgtccacg ttcgagatcg agtcggcgct ggtcgatcac tcggccgtcg
     4621 ccgaagcagc cgtggtgtcg cgccccgacg acgtggtggg ggaggcgatc gttgcgttcg
     4681 tgacgttgaa aggcaccgag cacggagacg aaaagctgat cgccgagctg cgcgagcacg
     4741 tcgccgccaa gatcggcaag atcgcccggc cgcactcgat cgtgctgacc gacgatttgc
     4801 ccaagacgcg atccggcaag atcatgcggc gtctgctgcg cgacgtcgcg ggcggccgtc
     4861 ccctgggcga cgcgacgacg ctcgcgaacg cggaagtcgt cgagcagatc agaaggctcg
     4921 ccgccgagcg accgaccgag gagtgagatg agcaatctag aacaggcacg ccggctgctt
     4981 caggccctgg agccggtcgc cgcagcgcac tggttcgcgc ccgactggcg cgaagagatg
     5041 gccgccgccg gcatcgacga ctacgcgccc gcgtacttct gctcgcgcgc cgcgccgctg
     5101 ggcccggtcc cgtggcaggt cgtgcacggg ctcttctaca actggaatcc cgcgctggtg
     5161 aagatggcgg tccgctggga cgtgtgcgct ccggccgacg cgtgggccgc gaaccttcgc
     5221 ggtgtgaacc gcgcgctgac ccgtgtgctg gccgcggtgg acggcgccga gatcgccaag
     5281 gccgccggga tgcttcgcga ggtcgcgacg ggctgtccgc aggaaggccg cgggctctac
     5341 gcgagctatg ccgcgctgcc gtggcccgac gctccggtcg aagcgctgtg gcaggcggcg
     5401 acggcgctgc gggagttccg cggcgacacg cacgtggcgg tgctggtgtc gcacgggctc
     5461 ggtccggtcg aggcgctcgt gctcaacgcc gcctggatcg gcgccgacct tcaacgctac
     5521 gccggcaccc ggatgtggag cgaggaggac ctgaaggaga cggtcgtcgc gctccaggat
     5581 cgcgcgttcc tcgacgccag cggcgcaatc accgacgggg gcggcaagtt ccgagagatg
     5641 atcgagatgg acaccgaccg ccgcgcgctg gcgccggtgg agatgctggg cgcggccgcc
     5701 gaggatgtgc tcgcggtgct cgagccgatc ggcaaggccg tgctcggcac gcctaaggcg
     5761 gttccgagca tcatcggcaa ggtcggacgt cccgcgacgg caggctagac gacacggacg
     5821 cggcgcggtc ctgcgatcgc cggggcttcg ccggcacgca ggatcacgcc ggtgtcgctc
     5881 tccatcgcta cgaccggcca cgacgcggtg gccgcgatct gctccagcca ttcgcgcggc
     5941 cgctcgtcgg catgcacggc gacggccacg ccggaaagcg gcccaagccc gtcggaccag
     6001 cgcgtgggca cgcgtgcgcc cgggacgggc agcgtgcaat gcgcgcacag cgccatcgcg
     6061 cccgcgctcg agcccgccag cgtcgcgccg ccctgccatc gctcgatcgc ctcgtcccac
     6121 aggggcgagc cgcgcaacgc gcgcagcagc gtgcgcgggc tgccgccgca caaaaacagc
     6181 acgtcgagct ccccgagcat gccgcgctcg gcgtgcccgc gttcgtagac cgggaccacg
     6241 aacgggtctg cgccgaagcg gcggtagtgg tcgatgccgt ggcgggcggc gtttggagcg
     6301 tcggcgccgg ctgcggcgac gatcagcgcg acgcgcgcac cgcgtgacgc ggcgagcatg
     6361 gcgtgatcga actcgtccgc ggccgggctg aactcgttgg acgacaggag cgcgaccggg
     6421 ccgcagcccg gcggaatggg cgggaaggcc ccggccagat cggtcacgcg ccgatcctag
     6481 tcgtcgtcct tgtctttgcc tttgcctttg cctttgtcct ggtcctggtc ctggttccgc
     6541 tcgcgaggcg gaggcgcggg cgccctggcg acgaccatgt ccaccggcgt tcccgctgcg
     6601 acggcctcgt ccgcggcggg gcgctgcgac agcaccgtgc ccggcggcgc gtccacggtg
     6661 aacacgaacg tgaccttgcc catccggagg ccggcatcct cgagcgtttt cttcgcgcca
     6721 tcgtaggtgg ccagagccgg caccgtgcag cagccgtcgg cggcgatcag acggagcgtg
     6781 gcccccggct tgaccgaagt gccggggccc gggtcttgcg agaagaccag cccttcgcgc
     6841 agcccgggcc tcggccgcgt ctcgacggcg acttccaggc cgagctgcgt ggccttcgcg
     6901 gacgcctgat cgacggtcat gttcgtcagc gacggcacgg cgaacgatcc gccgcgggag
     6961 agcgcggcgg tcagcccggt cgccacgagc gccaggatca gcgcgagcaa cgccgcacga
     7021 agcgcgacgc gacggagccg atgtggccgg ccggcattcg ggatcgcggc cgcgccggca
     7081 ggtgcggcgg ccgccccgag cacgaccgtg ccgggatcag aggagtccag acgcatcgtc
     7141 gcatccgcgg gcagcggcac gacggcgggc agcgtttcgt cgagcagcgc agcgcgcatc
     7201 tcggccgcgg tggcgaagcg gccggccggg tcgcgctcga gcgctttcat cgccacgctc
     7261 gacagcggca gcgcctcggg catcgagatc ggtgcgggat cttgcgacaa gcgcgccatc
     7321 gcaaccgtca ccggcgtgtc gcccgtgaac ggcagatggc cggtcaaagt ctcgtagagc
     7381 gcgacgccgg tttgatagac gtcggtttcg ggcgtggatc ccgcgccgcg gagctgctcg
     7441 ggcgccgcgt actgcggggt cgccatgatc gcgccggtca ccgtgagcgt ggactcgccg
     7501 gtcatcgtcg cgacgccgaa gtcggcgacc ttcacagtgc ccccggcggt gagcaggatg
     7561 ttggacggct tgatgtcgcg gtgcagcacg ccacagtcgt gcgcggcctg cacgccgtcg
     7621 aggacctgtg cgaagatccg ccgggcctcg tcgggggaga gcgggccacg cgcgatgcgt
     7681 tgcgcgagcg tctcgccttc gactagctcc atcacgatcg cacgccggtc ggtgtcctcg
     7741 acgtagtcgt agatgcttgc caccgccgga tgggagacct tgctcgcggc gattgcctcg
     7801 cggcggaagc gctcgcgtgc ggtctcatcg ccgtcgagcg tgccatgcag gatcttgacc
     7861 gccacgaggc ggccgagccg ctcgtcacga gctttccaaa cggtgcccat gcctccgccg
     7921 cggatctgct cgatcagccg gtatcggtct ccgaccagca ggttcaggtc tccacgggca
     7981 ggcgtgtcgg ttccgctcga acgattcatc cggcctaacg tacccggaaa ccgcctgctt
     8041 catccccggg gcttttccga tagcctcgcc ggtcgtgatc accctgaaga gccgcctcat
     8101 cgcccatatc cgggccaacg gtcctgtctc gttcgccgaa tacatgaccg cggcgctcta
     8161 cgatcccgac gacggcttct acgcgcgtcc tccggtcggt gcggccggcg acttcgtgac
     8221 cagcccgcac gtgagcgcgg ggttcggtct gctgctgagc cggcagatcg aaggcatgtg
     8281 gcgcgccgcc ggcgagccgg ccgattacgt cgtgaccgag gtcggggcgg gcgacggcac
     8341 gctcgccgcg cagatccgcg gcgcggccgc gggcgggttc gccgcggcgc tccgctaccg
     8401 cggcgtcgag gtcagcgccg gcgcgcgcgc cgcgctcgag caggcgggca tcgaagccgc
     8461 cgcctcgctc accgagctgg cgcccgcggc ggccggatgc atcctggcgc acgagctgct
     8521 cgacaacctt ccgttccacc ggctgcgggc acgcggtgac gacgtcgtcg aggtcttcgt
     8581 cgccgagcgt gacggcgccc tgatcgagat cgagggcccg ccctccgccg acgcggtcgc
     8641 ggcgcttcgc tggccgatgc tcgacggcga ggaacggccg gtctcgccgg cggcgcttcg
     8701 gttcctgcac gcggcgaccg gaatgctcga acgcggccac gtcctcatct acgactacgg
     8761 cttcggcgaa ggcgagcgct cgcagccggt gcgcgcctat gaagggcagc gtgtccacga
     8821 ccggctttac gacgatccgg gctcggcgga catcaccgcc ggcgtggact tcggcgcgct
     8881 cgtcgccgag gcccggtcgc tcggcctttc ggtccgcggg ccggcgatgc agcgcgacgt
     8941 gctcaaggcc ctcggcttcg acgactggtt cgacgatctg cgtgcgcggc gacggcaggc
     9001 cgaggccgac cgcgactcgc gcgcgatcct gcgtctgctg tcggagcaat cccgcgcgcc
     9061 gctgctgacc gaccccgcgc atctcggcgc gcatctggtg ctggccgccg gcatcggtga
     9121 cgcgggctcg ccaccgggtt tcggcgaagg cggcgaggcg taggcttcga gcatgcccaa
     9181 gaaggagcgt ctggcgcccg agccgttcac cgtcgccacg ccggtgccgc tggaggccga
     9241 gaagcgcggc gacgcgtggt ggacggttgt cgcggggcgc gagctgcgcc tttcgaacct
     9301 gcgcaagatc ttctggcccg agcagggcta caccaaaggc gacctgatcg cctactacca
     9361 cgcgatcgcg ccgatgatcc tgccctacct gtcggagcgg ccgttgacga tgaagcggat
     9421 gccggacggc atcgacgggc cgttcttcta cgaaaagtcc gcgccgtcgc acgttccgga
     9481 ctgggtgccg cgctgcccga tcccgagcga cgagggcggc gtcaccgatt acatgatcgc
     9541 cgccgacgag gcttgtctgc tcttcgtcgc gaaccttggt gcgatcgagt tccatccgct
     9601 gcactcgcgc tgcggatacg acgaccggcc cgactatctg ttcttcgacc tggatccgat
     9661 ggagaccggc ttcgacgacg tgcgcgtcgt cgcccggcac gtgcgcgcgg cgctggacgc
     9721 gctcgggctg ccgtcatatc cgaagacgag cggcgcaacc gggatgcaga tctacgttcc
     9781 gatcaagctc ggccccacgc acgagcagac gcgcgcgttc gtcggtgcga tcggccgcat
     9841 gatcctgaag gccgatcccg atcgcgtcac gatggaatgg gagatctcca agcgcaccgg
     9901 caaggtcttc atcgatcaca acatgaaccg gccgggcgcg aacatcgccg ccgtctacag
     9961 cgtgcggccc gagccgggcg cgaccgtttc cacgccggtg acgtgggacg aggtcgaagc
    10021 cggtgtccat ccgcgggact tcaccatcgt caccatctac gatcggctcg tcgcgacggg
    10081 cgatctgttc gaagggttgc tggccgagcc gatcgatctt cgtcccgcgc tcgagcggct
    10141 cggtttgccg agcgagatcg acgccgagct tccgctgcgc gagccgccga agccgcgtgt
    10201 gagcgtcgag cagggaacgc ccaaagaccg ccgcctcgcc gagtacatca agaaacgaga
    10261 cctggcggtc acgcccgagc ccggccccgg cgcggccggc gcggcgcgcg agagcggcga
    10321 catcttcgtg atccacaagc acaacgcgac gcggcttcac tacgaccttc gcctcgagca
    10381 cgacggcgcg ctcgagtcgt gggcagtgcc caaggggctg ccgaccgtgc cgggcgacag
    10441 gcggctcgcc gtgcacaccg agccgcaccc gatcgagtac gcggacttct ccggctggat
    10501 cccggagggc cattacggcg cgggcgaatc gctgatcttc gatcgcggca cgatcgacaa
    10561 gctcgaatgg acgcccggca aggtgacggt gcggctgcac ggcgagcgcg tgcgcggcga
    10621 ataccatctc gtcaagacca gccagggatg gctggtgttc ctgtcgaagg actcggccgc
    10681 cgagcagccc gacccgccgc cgcagctcgc gccgatgctc gccacggcgt ccaaagagcc
    10741 cttcgacgac ccgaagtggc gcttcgagcc gaagat
//
LOCUS       DATLFO010000051         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_139782, whole genome shotgun sequence.
ACCESSION   DATLFO010000051 DATLFO010000000
VERSION     DATLFO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139782"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VM841_01810"
     CDS             <1..1112
                     /locus_tag="VM841_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88948.1"
                     /translation="VEPSGMRSREVRRIVSSALALTVVLAVFGFARAADPSVQIRQRY
                     FGIENVDAVTGTVRPDRVILSWFGVTNFAAAIGGHVLILDAWIPRGEYSGYVPVSIPD
                     LVALDPEYVFLGHGHFDHAADSAPIVTGSGATLVSTPEHCDQAQRQAGDTPITCIAAV
                     PRGAAFGATTDLSTLMPGIGITAVKHVHSAAEPPDPSDPHTPAVPQPDANAVVQHLPA
                     PQDTVHLLSHLGDAEGGTVLYQFRVGNFSLTWHDSSGPLKEVAPGVLSTLAALPPTDV
                     QIGAIMGFNQITNGLRDPRMYIEAIKPKIFVPSHHDNWAPGVSTRGEHYKPFVEDELA
                     KIPAAYRPILRFISDPQDYVRPEVLTFDIEAPVWR"
     gene            complement(1388..>2031)
                     /locus_tag="VM841_01815"
     CDS             complement(1388..>2031)
                     /locus_tag="VM841_01815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL88949.1"
                     /translation="AGSPLAFFNQAIASRHLSDPPAAIEAATAFFEPRVPAYCLMIVG
                     DDRAAIAAAEASGFAARDSIPLMMLAPDELVAPAPPPELEIRPAADLSFVDLFGVGFV
                     DDVGARGPFHDVVRAAAAVDGWDLFGGFVDGEGVATSAAFTLDGCTGVFTVGTSPAAR
                     GRGIGAAMTVAAVGAGRARGSDTVFLFASAMGFPIYRRLGFRTVSSHRYIAQT"
BASE COUNT          318 a          754 c          656 g          303 t
ORIGIN      
        1 acgtcgaacc gtcgggcatg agatctcgag aggttcgccg gatcgtttcg agcgctctgg
       61 cgctcaccgt cgtcctggcc gtgttcggct tcgcgcgcgc cgccgacccg tcggtccaga
      121 tccgtcagcg ctatttcgga atcgagaacg ttgacgccgt gaccggcacg gtccgtcccg
      181 atcgcgtgat cctgtcgtgg ttcggcgtga cgaacttcgc cgcggcgatc ggcggccacg
      241 tcctgatcct cgacgcgtgg atcccccgcg gcgaatactc cggctacgtg ccggtgtcga
      301 tccccgatct cgtcgcgctc gaccccgagt acgtcttcct cggtcacggg catttcgacc
      361 acgccgcgga ctccgcgccc atcgtgaccg gaagcggcgc gacgctggtg agcacgcccg
      421 agcactgcga tcaggcccag cggcaggccg gcgacacgcc catcacctgc atcgccgcgg
      481 ttccgcgcgg cgcggcgttc ggcgcgacca cagacctctc gacgctgatg cccggcatcg
      541 gcatcaccgc ggtcaagcac gtgcattccg ccgccgaacc gccggacccg tcggacccgc
      601 acacaccggc cgtgccgcaa cccgatgcga acgcggtcgt ccagcacttg cccgccccgc
      661 aagacacggt gcatctgctc tcgcacctgg gcgatgccga gggcggaaca gtgctgtatc
      721 agttccgcgt tgggaacttc tcgctgacgt ggcacgactc gtcgggaccg ctcaaggagg
      781 ttgcgcccgg cgtgctaagc acccttgccg cgctcccacc caccgacgtg cagatcggcg
      841 cgatcatggg cttcaaccag atcaccaacg ggctgcgcga cccccgcatg tacatcgagg
      901 cgatcaagcc gaagatcttc gtcccgtcgc atcacgataa ctgggcgccc ggcgtttcca
      961 cccgcggcga gcactacaag cctttcgtcg aggacgagct cgcgaagatc cccgcggcgt
     1021 accgcccgat cctgcgcttc atctccgacc cgcaggacta cgtgcggccc gaggtcctta
     1081 cgttcgacat cgaggctccc gtctggcggt aagcgcgcga cggcgcggta ctggttcgac
     1141 gggatcttcc tccggcgccg gagctgcgcg cgccctggca tctcttggtg ttcctccgcg
     1201 tcgaccggcc gcgcaaccac catctgccgt cgagccggtg aacacgtccc catcggcgat
     1261 gttcatgcga cgtcgctcgc gatcgagata ctgcccgggt tcaccgcgcc tgcggagcac
     1321 cggcatgcgc caggtccgca gccagccgtg ccgtcccgat ccggtgcggg gcccgggccg
     1381 cgcgagctca ggtctgcgcg atgtagcgat gtgacgacac ggtccggaac ccgagtcgcc
     1441 ggtagatcgg gaaccccatg gcgctcgcga agaggaagac ggtgtccgat ccgcgcgcac
     1501 gtcccgcgcc caccgcggcc acggtcatcg ctgcgccgat cccacggccg cgcgcggccg
     1561 gcgacgttcc gaccgtgaaa acgccggtgc aaccgtccaa cgtgaacgcg gcagacgtcg
     1621 ccacgccctc gccgtccacg aacccgccga acagatccca tccatccact gcggccgccg
     1681 ctcggacgac gtcgtggaac ggtccccgcg cgcccacgtc gtcgacgaat ccgacgccga
     1741 acaggtcgac gaaagacagg tcggcggccg ggcggatctc cagttcggga ggcggcgccg
     1801 gagcgacgag ctcgtccggg gcgagcatca tcagggggat cgagtcccgc gccgcgaacc
     1861 ccgacgcttc ggccgccgcg atcgccgcac ggtcgtctcc cacgatcatg aggcaatatg
     1921 ccgggacgcg gggttcgaag aaggccgtcg ccgcttcgat cgcagccggg gggtcggaga
     1981 ggtgccggct cgcgatcgcc tggttgaaga acgccagcgg cgagccggca c
//
LOCUS       DATLFO010000052         3514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_41476, whole genome shotgun sequence.
ACCESSION   DATLFO010000052 DATLFO010000000
VERSION     DATLFO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41476"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VM841_01820"
     CDS             complement(<1..961)
                     /locus_tag="VM841_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HVL88950.1"
                     /translation="MSPFEIAQMNLTSPPVMAFGAGAVAVLARRNLRLPTALYTVLSG
                     WLLASIGIKGGVALSHTPLAAIALPAAATILLGTAIPVGVYALLRRRSRFGVADAAAM
                     AAHYGSVSAVTFTAAIAFLDATATPYEAFMPSLLVMLEVPAILVALTIARMRSGSSAG
                     WGHAVREVVTGRGIVLLAGGLVAGIAVGASGVAKVPSWFTAAFLIALTLFLLDMGMSA
                     ASRLRDFRVAGGYLTVFALAAPVVHGLAGVTLGTLVGMSAGGAAVLGIMAASASYIAA
                     PAAVRIALPEANPGYYLTTSIGITFPFNLVVGIPLMISVARWMA"
     gene            1087..1386
                     /locus_tag="VM841_01825"
     CDS             1087..1386
                     /locus_tag="VM841_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVL88951.1"
                     /translation="MPDKPEWTFLTNHAHVLLAIASDPEIRIRDVAERVGITERAAQR
                     IVSELEDGGYLTRHREGRRNHYEIHPRLPLRHPIEQHRDVGALLALIEPAKKRKR"
     gene            complement(1387..1989)
                     /locus_tag="VM841_01830"
     CDS             complement(1387..1989)
                     /locus_tag="VM841_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVL88952.1"
                     /translation="MTVPTREPHSERFAEALREAALWVSERGVARPRLAHGRLAATLV
                     VETEADPELAVAALLHDVAEHEIDDARMRAVRDRFGERVARVVAWCSENGTHPMPPFR
                     GLDEYRVAELLCATRDTLVVAAADTVAALRILVDDLGRGPVEAVSSPATAAAAIWFLG
                     EVAGAIQDRLPGHALAAETVAVTAVVTTALSRRAPLSAAS"
     gene            2109..2627
                     /locus_tag="VM841_01835"
     CDS             2109..2627
                     /locus_tag="VM841_01835"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVL88953.1"
                     /translation="MRVFLGSIETPFGEGLIAWTSRGVVALEAFVTGDEPGPSSRIRF
                     REIVTAIAGDDPRDGRLPDEMRGRALEAIDGDARRAPLDLRPFAPLRREVYRAVTRIP
                     RGSVASYADVARDIGRPRAWRAVGSALRRCPIDLLVPAHRVVHTGGTIGGGGASSIAW
                     KRTLLRREGVEV"
     gene            complement(2634..>3514)
                     /locus_tag="VM841_01840"
     CDS             complement(2634..>3514)
                     /locus_tag="VM841_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL88954.1"
                     /translation="VRASGIEAETIHSPGPPDDVVLGVASGRKADLIVVGNVGMRKAS
                     RIRMGPIPERIAREAPCDVLIVFTRDEPVAGTGDARRPYQGILVGVDGSPTAAEAART
                     SFDLAMMFRTEATLLYVAGDPMVGSIVLEQVMKQKPNATAAVTKIVEGDPAEKICGVA
                     RDEGIQLIVVGNRGLTGARRMVMGSVPSKVAHGSPCDVLIVKTSNMTLDDLAPGSGGL
                     VDVDGRKVAAYRNEDGSIVALSPRCQHMGCTVGWNGAEKTWDCPCHGSRYAKDGAVIQ
                     GPAAKALDPVDLDAAE"
BASE COUNT          547 a         1227 c         1262 g          478 t
ORIGIN      
        1 atgccatcca tcgcgccacc gagatcatca gcgggattcc cacgaccagg ttgaacggga
       61 aggtgattcc gatcgaggtc gtcaggtagt agccgggatt ggcctccggc agggcgatcc
      121 gcaccgcggc gggcgccgcg atgtaggacg ccgaggcggc catgatgccc agcaccgcgg
      181 caccgccggc ggacatgccc acgagcgtgc cgagcgtcac accggccagg ccgtgcacga
      241 ccggggcggc gagagcgaag acggtcagat agccgccggc gacgcggaag tcgcgaagcc
      301 gcgaggcggc ggacattccc atgtccagca ggaacagcgt cagcgcgatc aagaaggcgg
      361 ccgtgaacca cgacgggacc ttcgcgacgc cggacgcacc gaccgcgatg cccgcgacga
      421 gcccgcccgc caacagcacg atgccgcggc cggtgacgac ctcacgaacc gcgtgccccc
      481 agccggcgga ggagccgctg cgcatccgcg cgatggtcag cgccaccagg atcgcgggga
      541 cctcgagcat cacgagcagc gacggcatga acgcctcgta gggcgtggcg gtcgcgtcga
      601 ggaacgcgat cgcggcggtg aaggtgacgg cggagaccga gccgtagtgg gcggccatcg
      661 cggcggcgtc ggcgacgccg aaccggctcc ggcggcggag gagcgcgtag accccgacgg
      721 gaatggccgt tcccagcagg atcgtcgcgg ccgccggaag cgcgatcgcg gcgagggggg
      781 tatgcgacag cgcgacgccg cccttgatgc cgatcgaggc gagcagccat ccggaaagca
      841 cggtgtacag tgccgtcggg aggcgcaggt tgcgccgcgc gaggacggcg acggcgcccg
      901 cgccgaacgc catcacggga ggggaggtca ggttcatctg agcgatctcg aacggactca
      961 gcaggtcacc tcttccggcc ggacgcaccg gccggctccc ccgacaggcg ctttgcaata
     1021 caggaaatat ggttcctgta ttttgtatcg tatatcggac gagtgcgcaa gcaggtgagc
     1081 ccggcgatgc ccgacaagcc cgagtggacg ttcctgacca accacgccca cgtgctgctg
     1141 gccatcgcgt ccgaccccga gatccggatc cgtgacgtgg ccgagcgggt cggcatcacc
     1201 gagcgcgccg cgcagcgcat cgtgtccgag ctcgaagacg gcggctatct gacccggcat
     1261 cgggagggcc gccgcaacca ctacgagatc cacccgcggc tgccgctccg gcatccgatc
     1321 gagcagcacc gcgacgtcgg agcgctgctt gcgttgatcg agccggcaaa gaagcggaag
     1381 cgctgatcag ctcgcggcgc tgagcggcgc gcgccgggag agcgcggtcg tcacgacggc
     1441 cgtgaccgcg acggtctcgg ccgcaagggc atggccgggc aggcggtctt ggatcgcacc
     1501 ggcaacctca ccgaggaacc agatcgcggc ggcggccgtc gcgggcgagg agaccgcctc
     1561 gacggggccg cggcccaggt cgtccacgag gatccgcagc gccgccaccg tgtccgcggc
     1621 ggcgaccacg agcgtatcgc gggtggcgca caaaagttcg gccacccggt attcgtcgag
     1681 gccccggaac ggcggcatcg gatgcgtccc gttctcggag caccaggcca ccacgcgtgc
     1741 gacgcgctcc ccgaagcgat cccggaccgc acgcatccgc gcgtcgtcga tctcgtgctc
     1801 ggcgacgtcg tggagcagcg cggcgacggc aagctcggga tccgcctccg tctcgaccac
     1861 cagtgtggcc gccaggcgtc cgtgcgcgag ccgtgggcgg gcgacgcctc gctccgagac
     1921 ccagagcgca gcctcgcgaa gcgcttcggc gaagcgctcc gaatgcggct ctcgcgtggg
     1981 cacggtcatg tatctggaga acgacccgtc gatgtccttg tttcccgcat cggcccgata
     2041 tcgacgccgc tgaagaatga cacgcgtgtc attcgcatcg ctcgcttggg cgtgcgtagg
     2101 gttcattcgt gcgcgtcttc ctgggctcga tcgaaacgcc gttcggtgaa ggcttgatcg
     2161 cgtggacgtc ccggggcgtc gtcgcgctgg aggcgttcgt caccggcgac gagcccgggc
     2221 cgtcgtcgcg catccgcttc cgcgagatcg tgaccgcaat cgccggcgac gatccgaggg
     2281 acggacggct gcccgacgag atgcgcggcc gcgcgctgga ggccatcgac ggcgacgccc
     2341 gccgcgcccc gctggatctg cggcccttcg cgccgctccg gcgcgaggtg taccgcgcgg
     2401 tgacgcggat cccgcgcgga tcggtcgcgt cctacgccga cgtcgcgcgc gacatcggcc
     2461 ggccgcgggc ctggcgcgcc gtgggatcgg cgctgcggcg gtgccccatc gacctgctcg
     2521 tgccggcgca ccgcgtcgtg cacaccggcg gaacgatcgg cggcggcggc gcgtcctcga
     2581 tcgcgtggaa gcgcacgctg ctgcgccggg aaggcgtgga ggtctgagaa acgctactcg
     2641 gccgcgtcga ggtcgaccgg gtcgagagcc ttggcggccg ggccttggat gaccgcacca
     2701 tctttggcgt agcgggagcc gtgacaggga cagtcccagg tcttctcggc cccgttccac
     2761 ccgaccgtgc agcccatgtg ctggcaccgc ggcgacagcg cgacgatcga gccatcctcg
     2821 ttgcgatagg cggcgacctt gcggccgtcg acgtccacca gccctccgct gccgggcgcg
     2881 agatcgtcga gtgtcatgtt cgaggtcttg acgatcagca cgtcgcaggg cgagccgtgc
     2941 gcgaccttgc tcggcacgct tcccatgacc atgcgccgcg cgccggtcag gccgcggttg
     3001 ccgaccacga tcagctggat accttcgtcg cgcgcgaccc cgcagatctt ctccgccgga
     3061 tcgccttcga cgatcttggt cacggcggcc gtcgcgttcg gcttctgctt catcacctgc
     3121 tcgagcacga tcgagccgac catggggtcg cccgcgacgt agagcagcgt ggcctcggtg
     3181 cggaacatca tcgcgaggtc gaaggaggtc cgggccgctt ccgcggcggt gggtgagccg
     3241 tccacgccca ccaggatgcc ttggtagggc cggcgcgcgt cgccggtccc ggcgacgggt
     3301 tcgtccctgg tgaacacgat cagcacgtcg cacggcgcct cacgggcgat gcgctccggg
     3361 atcggaccca tgcgaatccg gctcgccttc cgcatgccga cgttgccgac aacgatgaga
     3421 tcggccttgc ggccggaggc gacgccgagc accacgtcgt cgggcggacc cgggctgtgg
     3481 attgtctcgg cctcgatgcc gctggcgcgc acca
//
LOCUS       DATLFO010000053         5595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_1010, whole genome shotgun sequence.
ACCESSION   DATLFO010000053 DATLFO010000000
VERSION     DATLFO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VM841_01845"
     CDS             complement(<1..892)
                     /locus_tag="VM841_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL88955.1"
                     /translation="MTPPAEGPFEGHGGDFVAEAMRGANIDRVFTLSGGHIFPIYDGM
                     HTRGMRIIDVRHEQTAGFAAEAHAKLARVPGVAALTAGPGVTNGVSPIASAHFNGSPL
                     VVLGGRAPQLRWGQGSLQEMDHVPIVASITKHAETIFDPAAIGSAAARAFAVAAQPHR
                     GPVFLDVPLEVLFTRCTTSVEQTAPATPAIDGDVEAAVRALSQAERPVLLIGSDVWAD
                     GAEKAARDLVERLRIPAFMNGMGRGVLPADHELVFSHARGHAFKRADLVIVAGAPLDF
                     RLGFGKFGDAKVIHLTDHPAL"
     gene            complement(889..2370)
                     /locus_tag="VM841_01850"
     CDS             complement(889..2370)
                     /locus_tag="VM841_01850"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HVL88956.1"
                     /translation="MPKFRLVKESPEAVACDLLAVAVFKDAKPGPGAAEVATLLGTTL
                     KDLFAAERISGNVGDCAVLSTVGHIAAKRVAFVGLGDKTAVTPFEIRRAGAVVARKAA
                     GAKTVGTLVPAAASGNGTDAARAFAEGFLLGSYKFTRYKKASANGSKPVAVSDVLIAG
                     MAKSAGIDKSLAAAQAVADGTVLARDLTNTPANDKSPVSLAQQARDIAKGTGMTVKVM
                     DEKQLAAKGMNGILGVGRGSPKPPRLIEINYKATGAKKNIAIIGKGITFDSGGINLKT
                     QGLDWMKMDMGGAAAVLGIMKAIAAIKPKVNVTAIICSAENMPGGNALHPGDVLDMYG
                     GKTVEIGNTDAEGRLVMADGLALAAERKKYHYILDMATLTGACMVALGPRCFGVMGND
                     DGLIQGLLGASKRAGELAWELPLIEDYRKMIDSDIADLKNIGGPYGGAITAGLFLREF
                     IGDHKWAHMDIAGPTKSESDEFEQPKGATGSGVRTVVEWLTSL"
     gene            complement(2458..4152)
                     /gene="thiC"
                     /locus_tag="VM841_01855"
     CDS             complement(2458..4152)
                     /gene="thiC"
                     /locus_tag="VM841_01855"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVL88957.1"
                     /translation="MSDLLETTTPRATAPRARKVYVPGSRPDLRVPMREITPGGEEPL
                     RLYDTSGPWTDPDVSVDVRTGLPELRLPWILERGDVEEVTHAAAPVAGFDRRHVLRAT
                     DGATVTQMHYARLGIVTPEMEFVAIRENVDPEFVRAEIAAGRAIIPANVNHPESEPMI
                     IGRNFLVKINANIGNSAVASSIEEETAKMKWATRWGADTLMDLSTGAQIHETREWIIR
                     NCPTPVGTVPIYQALEKVGGDPAALSWEVYRDTIIEQCEQGVDYFTVHAGVLLRFVPM
                     TAKRVTGIVSRGGSIMAAWCLAHHQENFLYTNFEELCEILARYDVSFSLGDGLRPGCI
                     ADANDDAQLGELKTLGELTTIAWSRDVQVMIEGPGHVPMNKIKENMDLQLEWCHEAPF
                     YTLGPLVTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNAQDVKEGVITY
                     KIAAHAADLAKGHPGAQAWDDALSRARFEFRWDDQFDLSLDPDTARDYHDETLPAEPA
                     KKAHFCSMCGPNFCSMKITQDVRDYAEQHGLTSEEALIAGMREKSAEFRERGAIYE"
     regulatory      complement(4142..4257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4357..4665
                     /locus_tag="VM841_01860"
     CDS             4357..4665
                     /locus_tag="VM841_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HVL88958.1"
                     /translation="MTVRMAIYYKQPDDPETFEKRYLEEHLPLVRAYDNVKVTKFHKV
                     GRLIFGEQPQFAYVFVGAWDDKESWKADMQSEAAKKATEHAQSLGVPFDVVMLDELTE
                     "
     gene            4675..5463
                     /locus_tag="VM841_01865"
     CDS             4675..5463
                     /locus_tag="VM841_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL88959.1"
                     /translation="METVLYDIANGVATITMNRPDALNSVDGTLGSELLKALQDAAGD
                     AQVRCVVLTGAGKGFCAGADLGQFQEAFDAGVAPPMNEVLRERYHPLITSITEMAKPV
                     VGGLNGVAAGMGTSLAMACDFRIASDKARFTQAFLKIAVVPDSGATHLLPQLVGVAKA
                     KELAMLNPVLNADEMLRIGLVTEVVPHEQYPARLAEFAAALAKGPTLALGLTKKAINH
                     GARQDLLSSMDYEADLQQQIAHSADMTEGIKAFLEKREANYTGR"
BASE COUNT          876 a         1868 c         1893 g          958 t
ORIGIN      
        1 tcaacgccgg atggtcggtc aaatgaatca ccttcgcgtc accaaacttg ccgaagccca
       61 aacggaagtc cagcggcgcg ccggcgacga ttaccaggtc ggcgcgtttg aacgcgtggc
      121 cccgcgcgtg cgagaagacc agctcgtggt ccgcgggcag cacgccgcgg cccatgccgt
      181 tcatgaacgc gggaatgcga agccgctcga ccagatcgcg tgcggctttc tcggcgccgt
      241 cggcccacac gtcgctgccg atcagcagca cgggacgctc ggcttgcgac agcgcgcgca
      301 cggcggcctc gacgtcgccg tcgatcgcgg gcgtcgcggg cgcggtctgc tcgaccgacg
      361 tggtgcagcg ggtgaacagc acctcgagcg gaacgtccag gaacaccggt ccgcgatgcg
      421 gctgcgcggc aaccgcgaag gcgcgggctg cggccgagcc gatcgcagcg gggtcgaaga
      481 tcgtttcggc atgcttggtg atcgaggcca cgatcgggac gtgatccatc tcttgcaggc
      541 tgccctgacc ccagcggagc tgcggcgcgc gcccgccgag cacgaccagc ggcgagccgt
      601 tgaagtgcgc cgatgcgatc gggctgacgc cgttggtcac gcccggcccg gcggtcagcg
      661 ccgcaacgcc cggcacccgc gccagctttg cgtgcgcttc ggcggcgaag ccagcggtct
      721 gctcgtggcg aacgtcgatg atgcgcatgc cgcgcgtgtg catgccgtcg tagatcggaa
      781 agatgtgccc gcccgacagc gtgaagacgc ggtcgatgtt cgcgccgcgc atcgcttcgg
      841 cgacgaagtc gccaccgtgg ccttcgaacg gcccctctgc cggtggggtc acagcgacgt
      901 gagccactcg accacggtgc gcacgcccga gccggtcgcg cccttgggtt gctcgaactc
      961 gtcggattcg ctcttggtcg gaccggcgat gtccatgtgc gcccacttgt ggtcgccgat
     1021 gaactcgcgc aggaacagcc cggcggtgat cgcgccgccg taggggccgc cgatgttctt
     1081 gagatcggcg atgtcgctgt cgatcatctt gcggtagtcc tcgatcagcg gaagctccca
     1141 ggccagctcg ccggcgcgct tggacgcgcc cagcagacct tggatcaatc catcgtcgtt
     1201 gcccatcacc ccgaagcagc gcggccccag cgcgaccatg cacgcgccgg tcagcgtcgc
     1261 catgtccagg atgtaatggt acttcttccg ctcggccgcc agcgccagcc cgtcggccat
     1321 gaccagccgg ccttcggcgt cggtgttgcc gatctcgacg gtcttgccgc cgtacatgtc
     1381 cagcacgtcg cccgggtgca gcgcgttgcc gccgggcatg ttctcggccg agcagatgat
     1441 tgcggtgacg ttgaccttcg gcttgatcgc ggcgatcgct ttcatgatgc cgagcaccgc
     1501 cgcggccccg cccatgtcca tcttcatcca gtccaggccc tgggtcttca agttgatgcc
     1561 gcccgagtcg aaggtgatgc ccttgccgat gatggcaatg ttcttcttcg cgccggtcgc
     1621 cttgtagttg atctcgatca ggcgcggcgg cttgggcgag ccgcgaccca cgcccaggat
     1681 tccgttcatg cctttggcgg cgagctgctt ttcgtccatg accttgacgg tcatgccggt
     1741 gcccttcgcg atgtcgcgtg cctgctgcgc gagcgagacc ggcgacttgt cgttggcggg
     1801 cgtgttcgtc aggtcgcgcg ccagcacggt gccgtcggcg acggcctgcg cggcggcgag
     1861 cgacttgtcg atgccggcgc ttttcgccat gccggcgatc agcacgtcgc tgaccgcaac
     1921 cggcttcgat ccgttcgccg atgccttctt gtagcgcgtg aacttgtacg agccgagcag
     1981 gaagccttcc gcgaatgcgc gggccgcgtc ggttccgttt ccgctggcgg cggcgggcac
     2041 caacgttccg accgttttcg cgcctgcggc cttgcgcgcg acgaccgcgc cggcgcggcg
     2101 tatctcgaac ggcgtgacgg ccgtcttgtc gcccaggccg acgaacgcga cgcgcttcgc
     2161 ggcgatgtga ccgaccgtgg acagcaccgc gcagtcgccg acgtttccgc tgattcgctc
     2221 ggcggcgaac aggtctttga gcgtcgtgcc caaaagcgtc gcgacctcgg ccgcgccggg
     2281 cccgggcttc gcgtccttga agaccgcgac ggccagcaga tcgcaggcga cggcctcggg
     2341 agactctttg accagacgga acttcggcat gtcagaactc ctcgctcatg cggtgtggaa
     2401 agacgccgaa ttatacgcac ggccgggccc ggtcctgccg ggccgcccgg atcgggccta
     2461 ttcgtagatc gcgccgcgct cgcggaactc ggcggacttt tcgcgcatcc cggcgatcaa
     2521 ggcctcttcg ctggtcagac cgtgctgctc ggcgtagtcg cgcacgtcct gggtgatctt
     2581 catcgagcag aagttcggcc cgcacatcga gcagaaatgc gccttcttgg ccggctcggc
     2641 cggcaaggtc tcgtcgtggt agtcgcgggc ggtgtccggg tccagcgaca ggtcgaactg
     2701 gtcgtcccac cggaactcga agcgagcccg cgacagcgcg tcgtcccacg cttgcgcgcc
     2761 ggggtggcct ttggccaggt ccgcggcgtg cgcggcgatc ttgtaggtga tcacgccttc
     2821 cttgacgtcc tgcgcgttcg gtaggccgag atgctccttc ggcgtgacgt agcagagcat
     2881 cgcggtgccg taccagccga tcatcgcggc gccgatcgcg ctggtgatgt ggtcgtagcc
     2941 gggcgcgatg tcggtgacca gcggcccgag cgtgtagaac ggcgcctcgt ggcaccactc
     3001 cagttgcagg tccatgttct ctttgatctt gttcatcggc acgtggcccg ggccttcgat
     3061 catgacttgc acgtcgcggg accacgcgat cgtggtcagc tcgccgaggg tcttgagctc
     3121 acccagctgc gcgtcgtcgt tggcgtcggc gatgcagccc ggccgcagcc cgtcgcccag
     3181 cgagaacgac acgtcgtagc gggcgaggat ctcgcacagc tcttcgaagt tggtgtacag
     3241 gaagttctcc tgatggtgcg ccaggcacca tgccgccatg atcgagccgc cgcgcgagac
     3301 gatgccggtg acccgcttgg cggtcatcgg cacgaagcgc agcagcacgc ccgcgtgcac
     3361 cgtgaaatag tccacgccct gctcgcattg ttcgatgatc gtgtcgcggt agacctccca
     3421 cgacagcgcc gccggatcgc cgcccacctt ttcgagcgcc tgatagatcg gcaccgtgcc
     3481 gaccggcgtg gggcagttgc ggatgatcca ctcgcgcgtc tcgtggattt gcgcgccggt
     3541 ggacaggtcc atcagcgtgt cggcgcccca gcgggtcgcc cacttcatct tcgcggtctc
     3601 ctcctcgatc gacgaggcga ccgcggagtt gccgatgttg gcgttgatct tcaccaagaa
     3661 gttgcggccg atgatcatcg gctcggactc ggggtggttg acgttcgccg gaatgatcgc
     3721 gcgaccggcc gcgatctcgg cgcgcacgaa ctcgggatcg acgttttcgc gaatcgcgac
     3781 gaactccatc tcgggcgtga cgatgccgag gcgcgcgtag tgcatctgcg tgacggtggc
     3841 gccatccgtg gcgcgcagca cgtgacgccg gtcgaagccg gcgaccggag cggcggcgtg
     3901 cgtgacctcc tcgacgtcgc cgcgctccaa aatccaggga aggcgcagct cgggcaggcc
     3961 ggtgcgcaca tcgaccgaca cgtcggggtc cgtccacggg ccgctggtgt cgtacaagcg
     4021 aagcggctcc tcgccgccgg gcgtgatctc gcgcatcggc acccgcagat cgggccgcga
     4081 gcccgggacg tagaccttgc gggcgcgcgg cgccgtcgcg cgcggcgtgg tggtttccag
     4141 caggtcgctc atctcgattt ccctccgccg gcattacccg gatcaggttg tttcgggtcg
     4201 gcggatccca tccgccctct cagcccggtc acattccgag ctccccggtc gtcgggaacc
     4261 agcctactcc ttctcggcgg acgggcgcgt cggatgtgac atcggcacga tcggcgacgg
     4321 acaatgagcg catcggccga ccacgaggag gcagcgatga ccgttcggat ggcgatctac
     4381 tacaagcagc ccgacgatcc ggaaacgttc gagaagcgct acctggagga gcaccttccg
     4441 ctggtccgcg cctacgacaa cgtcaaggtc acgaagttcc acaaggtggg ccgcctgatc
     4501 ttcggcgagc agccacagtt cgcgtacgtc ttcgtcggcg cctgggacga caaagagagc
     4561 tggaaagccg acatgcaaag cgaggccgcg aaaaaggcga ccgagcacgc gcaatcgctg
     4621 ggcgtgccgt tcgacgtcgt gatgctcgac gagctcaccg aataggagcc cggcatggag
     4681 accgttctgt atgacatcgc aaacggcgtc gcgacgatca ccatgaaccg tccggacgcg
     4741 ctgaactcgg tggacggcac cctgggatcc gagctgctga aggcattgca ggatgcagcg
     4801 ggcgatgcac aggtgcgctg cgtcgttctg accggcgccg gcaaaggatt ctgcgccggc
     4861 gccgacctgg gccagtttca agaagcgttc gatgccggcg tcgcgccgcc gatgaacgag
     4921 gtgctgcgcg agcgctacca cccgctgatc acctcgatca ccgagatggc caaaccggtc
     4981 gtcggaggtc tgaacggcgt cgctgccggc atgggcacgt cgctggcgat ggcatgcgac
     5041 ttccggatcg catccgacaa ggcacgcttc acacaagcgt tcttgaagat cgcggtcgtg
     5101 cccgactcgg gcgcgacgca tctgctgccg caactggtcg gggtcgcgaa ggcgaaagag
     5161 ctggcgatgc tgaatccggt gctgaacgcc gacgagatgc tgcgcatcgg cctggttacc
     5221 gaggtcgtgc cccacgagca gtatccggcg cggctggccg agttcgcggc cgcattggcc
     5281 aaaggaccga cgctggcgct gggcctgacg aagaaggcaa tcaatcacgg ggcgcgccaa
     5341 gacttgctgt cctcgatgga ttacgaagcc gacctgcagc agcagatcgc gcactcggcc
     5401 gacatgaccg aaggcatcaa ggcgttcctg gagaagcgcg aagcgaacta cacgggacgc
     5461 tgatgccgca gcaggttcgc aaccccatct tcggccgcgt ctacgcggct ctggccggct
     5521 cgccgctcgg caaggcgatg gagaaggacc ggcgccggct gctcgacggg ctgtcgggac
     5581 gcgtcgtgga agtcg
//
LOCUS       DATLFO010000054         7500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_100948, whole genome shotgun sequence.
ACCESSION   DATLFO010000054 DATLFO010000000
VERSION     DATLFO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100948"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VM841_01870"
     CDS             complement(<1..572)
                     /locus_tag="VM841_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL88960.1"
                     /translation="MSGSVVITGASTGIGRACALAMDGKGWRVFAGVRNETDAASLRD
                     AASERLTPLILDVTDQEHIHEAVAVLSEAVGETGLAGLVNNAGIAVAGPMEFVPLDDL
                     RRQFEINVIGLVATTQAFLPLIRKATGRIVHMGSVGGSFAVPFAGPYSANGTANDPPT
                     EPMCTIRPVAFRISGRNACVVATRPITLIS"
     gene            649..1803
                     /locus_tag="VM841_01875"
     CDS             649..1803
                     /locus_tag="VM841_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL88961.1"
                     /translation="MEFAFTPEQQEFRASVRRFAEDVIAPRAEEMDETEEFPTDIVDQ
                     MGKMGLFGIPFPEEYGGMGGDFTTLCIAIEELARVDSSMAITLEAGVGLGAMPIYLFG
                     TEEQKQQWLVPLARGEKIAGFGLTEPNAGSDSGGTQTKAVLDGGEWLINGSKSFITNV
                     GTPNSLFCTITAVTGEANGRKEISNIVVPLGTPGFTVGRKYKKMGWHASDTRELSFSD
                     VRVPEANLLGTRGEGFKNFLIILDGGRVAIGALAVGLAQGCLEMSINYAKERKQFGRP
                     IADFQAVQFKIADMAVDTDLARLAVYRAAWMHDQGMPFKKEASIAKLFSSEIAVTAAR
                     NAVQVHGGYGFMNEYPVSRFYRDSKVLEIGEGTSEVQRLLIARELGLTDA"
     gene            complement(1800..2030)
                     /locus_tag="VM841_01880"
     CDS             complement(1800..2030)
                     /locus_tag="VM841_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88962.1"
                     /translation="MLQGAFHPMDGSPATLAPLDRPEGLTLDANGHLLIAEAGGNAIR
                     LADVGANAWRRGASLLQSGMAYDTTLQQRFRR"
     gene            complement(2087..2680)
                     /locus_tag="VM841_01885"
     CDS             complement(2087..2680)
                     /locus_tag="VM841_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88963.1"
                     /translation="MEAGGKAKAGLIAWLLPVLLGVGGSIGAVAAFGEGTYDVAPFDV
                     KMKVSFGSQGQTRLGIEAPALGNPGVAVADTHSGPLRFNGTITGVDAGSLATAVVQTA
                     GARDEQLNPADPRSVAVFVAQNGKSAARSFGIKVGLIAAGGSLIGGLAGALGKPKQAI
                     AGLIAGVAVVGVLGLVAGQTYDTSAFEGTRFEQTSSG"
     gene            complement(2681..3865)
                     /locus_tag="VM841_01890"
     CDS             complement(2681..3865)
                     /locus_tag="VM841_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HVL88964.1"
                     /translation="MEQRRTLKDLLTDAKDESEFMVDLAYAAVFFGDEAIAQEVLQLE
                     ESMDEIVHELRTIAVLAGRSREDAEALAGVMRLAGAMEKIADAAQDIARVVLKDLGVP
                     PGLRDDLRHAEEAVARIRLRDENDLEGKTLEELELPTHTGMQVIAIRRGVRFLFDVNG
                     DTMIQAGDVLFVQGPEEGIDLMRQLAGAPPRHLREPVAKTLTGIDRAVDLIVEMKNST
                     EVAVGLAYSAILFRDKGLAAEVFAIEDKSDEMLHELERWVLRATVEVEDPDELRGLLH
                     LAAASERIGDAAQDMMRLVEGEDEIHPIIAEALAEADEIVAEATVAAEAPATGRTLRD
                     LKLRTETGLDVLGVSRGGRWIYRPRAEFTLLAGDRVLAVGPEDGAEALDELCGVVRDE
                     ED"
     gene            complement(3868..5187)
                     /locus_tag="VM841_01895"
     CDS             complement(3868..5187)
                     /locus_tag="VM841_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HVL88965.1"
                     /translation="MKKPTPEERRRLSRVPAQAARTVARAPAAAVRAPARAVVQIGRY
                     WRAERTTLRQGFVALWISSGGDLLAGLALGAMTHRLEELPGLLILVPAVIGMRGSIFG
                     ALGSRLGTSIHAGLFRLSMNRTGVLYQNAYAATLLSITSAAFLAVAARVMTSALGLPT
                     VPVWDFLAISVVAGTVSGGVILVLTILLARTAYRRDWDLDSVAAPVVTFMGDIITLPS
                     LFAASFLARQQGVTVAVGVATAAVSLAALAMGLRTKLPAARRIMRESVVMLMLTGVVM
                     LIAGAVIEHRRDRFLALPALLVLIPPFLEDAGALGGIVSSRLASKLHLGAIRPKLIPE
                     RLALLDISLLAPFALSVFTLVGLSADLVARAVGLESPGLLTMVSVSLLGGLLATLGAA
                     VIAYAAAVATFRFGLDPDNHAIPIVTSSVDLVGMICLIVAIVVLKVS"
     gene            complement(5268..5486)
                     /locus_tag="VM841_01900"
     CDS             complement(5268..5486)
                     /locus_tag="VM841_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88966.1"
                     /translation="MYPPFEYDMIRERREELWRMADQTRLAKKAARREHRRAEHRPAP
                     IAAIGSALIRAGRALGGREPLRSPAHGS"
     gene            5606..6160
                     /locus_tag="VM841_01905"
     CDS             5606..6160
                     /locus_tag="VM841_01905"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HVL88967.1"
                     /translation="MTVKADTVPAPTRLVAAFVNTLDLESPADRIGTPAALRAWLQEQ
                     SLLPEDAPLPGRYDVDMAQAMREDLRDLLECNCGGPLAERCVNGINRVSSAGRLVVRI
                     GPDGRAVLEPETAGVSGALGAIMAAVYTAMTDGTWERLKTCRRESCRWVFYDASKNRS
                     GQWCSMKVCGNREKARQYRERHKA"
     gene            6157..7077
                     /locus_tag="VM841_01910"
     CDS             6157..7077
                     /locus_tag="VM841_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HVL88968.1"
                     /translation="MTAFDVPMHGAGGEPVDLWRTINSHGVASLPPHVVDEDARTLTT
                     VVALAGTKPRRVRIAAGRGRTARVTIDGPAAGERTVARVRGAVRHILALDRDLSAFYA
                     LVADDPALAWTVRGAGRMVRSQTVFEEVVKTICTTNCAWSATERMVGALVEHLGEPAA
                     GVKPGPGPAGRAFPTPAAMAEADDDFYRGVVRAGYRGRYFRALARDVADGTLDLEALA
                     TDDETSDEDVYKQLIALPGVGPYAAAHIMQMIGRHSRPILDSWTRPTYARLTGKKSVT
                     DKAILRRFARYREHAGLAFWLFLTQDWVAE"
     gene            complement(7078..>7500)
                     /locus_tag="VM841_01915"
     CDS             complement(7078..>7500)
                     /locus_tag="VM841_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HVL88969.1"
                     /translation="FQNTIAYVYRQSLEDPGAQVTRIRVRSLLMPPGFPDFVTRTRIV
                     DAGPVMIRGRAWSGSGPIVRVELGVDGAWTEAKLDEPIDDFAWRGWSAEWIAEPGEHV
                     LSCRATDESGDTQPVDEAPWNYGGFGNNLIHTVPVTVR"
BASE COUNT         1208 a         2603 c         2538 g         1151 t
ORIGIN      
        1 ttcgaaatca acgtgatcgg cctcgtggcg acgacgcaag cgttcctgcc gctgatccga
       61 aaggcgaccg gccggatcgt gcacatgggc tcggtcggcg gatcgttcgc ggtgccgttc
      121 gccgagtagg gcccggcgaa cggcaccgcg aacgatccgc cgaccgagcc catgtgcacg
      181 atccggccgg tcgcctttcg gatcagcggc aggaacgctt gcgtcgtcgc cacgaggccg
      241 atcacgttga tttcgaactg gcggcgcaga tcgtcgagcg gcacgaactc catcggcccg
      301 gcgaccgcga tcccggcgtt gttgaccagc ccggccaggc cggtctcacc cacggcctca
      361 ctcaggaccg cgaccgcctc gtggatgtgc tcctggtcgg tcacgtcgag gatcagcggc
      421 gtgagccgtt cggaggccgc gtctcgcagc gaggccgcgt cggtctcgtt gcgcacgccc
      481 gcgaagaccc gccagccctt cccgtccatc gcgagcgcgc aggcgcgccc gatgcctgtc
      541 gaagccccgg tgatcacaac cgatccactc atgtgccgtc cctcccgggc tcaccctact
      601 cccgcccgtc gcccgcgata gtctttgccc gaacggagga tcccgatcat ggaattcgca
      661 ttcacgcccg agcagcaaga gtttcgcgcc agcgtccggc gcttcgccga ggacgtcatc
      721 gcgccgcgcg cggaagagat ggacgagacc gaggagttcc cgaccgatat cgtcgaccag
      781 atgggcaaga tgggcctgtt cggcattcca ttcccggagg agtacggcgg gatgggcggc
      841 gacttcacca cactgtgcat cgcgatcgaa gagctcgcgc gcgtggactc gtcgatggcg
      901 atcacgctcg aggccggcgt cggtttgggt gcgatgccga tctatttgtt cggcaccgaa
      961 gagcagaagc agcagtggct ggtgccgctg gcgcgcggcg agaagatcgc cggcttcggt
     1021 ctcaccgagc cgaacgcggg atccgactcc ggtggcacgc agacgaaggc ggtgctcgac
     1081 ggcggcgagt ggctgatcaa cgggtcgaag tcgttcatca ccaacgtcgg cacacccaac
     1141 tcactgttct gcaccatcac cgcggttacc ggagaagcga atggccgtaa ggagatctcg
     1201 aacatcgtcg tgcccctcgg cacgcccggc ttcacggtcg gccgcaagta caaaaagatg
     1261 ggctggcacg cctcggacac gcgtgagctt tccttcagcg acgtgcgggt gcccgaggcg
     1321 aaccttctcg gcacgcgcgg cgagggcttc aagaacttcc tgatcatcct ggacggcggc
     1381 cgggtcgcga tcggtgcgct ggcggtcggc ttggcgcagg gctgccttga gatgtccatc
     1441 aactacgcaa aggagcggaa gcagttcggc cgtcccatcg cggactttca ggcggtgcag
     1501 ttcaagatcg cggacatggc ggtggatacc gacctggcgc ggctcgcggt ctatcgcgcc
     1561 gcgtggatgc acgaccaagg catgcccttc aagaaggaag cctcgatcgc caagctgttc
     1621 tcgtccgaga tcgccgtcac cgcggcgcgc aacgcggtgc aggtgcacgg cggctacggc
     1681 ttcatgaacg agtatccggt cagccgcttc taccgggact cgaaggtgct cgagatcggc
     1741 gagggcacca gcgaggtgca gcgcctgctg attgcgcgcg agctcggcct caccgacgcc
     1801 taacgccgaa atcgttgttg caaagtggtg tcgtatgcca tgccggattg caacaacgac
     1861 gcccctcgcc gccaggcgtt cgcgccgacg tcggccagcc tgatcgcgtt gccgccggct
     1921 tcggcgatca gcaggtgtcc gttcgcgtcg agcgtaaggc cctcgggccg atccagaggt
     1981 gcgagcgtcg ccggagagcc atccatcgga tggaacgcac cctgcagcaa ggtggcggcg
     2041 gtctaaccgt cgcggcccgg caagggccct ggtcgcgtcc gctctgttag ccggacgagg
     2101 tctgttcgaa gcgcgtccct tcgaaggcgg acgtgtcgta ggtctggccc gcgaccagcc
     2161 cgagcacgcc gaccacggcg acgccggcga tcaggccggc gatggcttgc ttgggcttgc
     2221 ccagtgcgcc cgccagcccg ccgatcaggc ttccgcccgc cgcgatcaag ccgaccttga
     2281 tgccgaacga gcgtgcggcg ctcttgccgt tctgcgcaac gaacaccgcg accgaacgtg
     2341 gatccgccgg attcagctgc tcgtcgcgag ccccggcggt ctgcacgacc gcggtcgcca
     2401 gcgatcccgc atccacgccg gtgatcgttc cgttgaatcg cagcggaccc gaatgggtgt
     2461 cggcgaccgc cacgcccggg ttgcccagag ccggcgcttc gattcccaga cgcgtctgtc
     2521 cctgcgagcc gaagctcacc ttcatcttca cgtcgaacgg cgcgacgtcg taggtgccct
     2581 cgccgaacgc cgcgacggcg ccgatcgagc cgccgacgcc cagcagcacc ggcagcagcc
     2641 acgcgatcag cccggccttg gccttgcccc ccgcctccat ctagtcctcc tcgtcgcgga
     2701 ccacgccgca cagctcgtcg agcgcctcgg cgccgtcttc gggtccgacc gcgagcacgc
     2761 ggtcgccggc cagcagcgtg aactcggcac gcggccggta gatccagcgc ccgccgcgcg
     2821 acacgccgag cacgtccagc ccggtctcgg ttcgaagctt caggtcgcgc agcgtccgtc
     2881 cggtcgctgg cgcttcggcc gcgacggtcg cttcggccac gatctcgtcg gcctcggcca
     2941 gcgcttcggc gatgatcgga tggatctcgt cttctccttc gaccagccgc atcatgtcct
     3001 gcgcggcgtc gccgatgcgc tcggaggccg cggccaggtg cagcaggccg cgaagctcat
     3061 cgggatcttc gacctcgacg gtcgcgcgca gcacccaccg ttcgagctcg tgcagcatct
     3121 cgtcggactt gtcctcgatc gcgaacactt cggctgcgag tcccttgtcg cggaacagga
     3181 tcgccgagta cgcgagcccg accgcgacct cggtggagtt cttcatctcc acgatcagat
     3241 cgacggctcg gtcgatgccg gtaagcgtct tggcgaccgg ctcgcgcaga tgccgcggcg
     3301 gcgcgcccgc gagctgtcgc atgaggtcga tgccttcctc gggcccctga acgaacagca
     3361 cgtcgcctgc ctggatcatg gtgtcgccgt tgacgtcgaa caggaatcgc acgccgcgcc
     3421 ggatcgcgat gacctgcatg ccggtgtgcg tgggaagctc gagttcttcg agcgtctttc
     3481 cttccaggtc gttctcgtcg cggagccgga tacgcgcgac ggcctcctcg gcgtgacgca
     3541 gatcgtcgcg caggcctggc ggtacgccga ggtccttgag cacgacgcgc gcgatgtctt
     3601 gggcggcgtc ggcgatcttt tccatcgcgc ccgccaggcg catgacgccc gcgagcgctt
     3661 cggcgtcctc gcggctgcgc ccggccagca ctgcgatcgt gcgaagctcg tgcacgatct
     3721 cgtccatcga ctcttccagc tgcaggacct cttgcgcgat cgcctcgtcg ccgaagaaca
     3781 ccgcggcgta ggcgagatcg accatgaact cgctctcgtc cttggcgtcg gtcagcaggt
     3841 ccttcagcgt tcttcgttgt tccatcgtca actcactttc agcacgacga tcgccacgat
     3901 caagcagatc atgccgacca gatccaccga gctggtcacg atcgggatcg catggttgtc
     3961 gggatccagc ccgaatcgga acgtcgcgac ggccgcagcg tatgcgatca cggcggcgcc
     4021 gagcgtggcc agcagcccgc ccagcagcga cacggacacc atcgtcagaa ggccggggga
     4081 ctccaggccc acagcgcgcg cgaccagatc ggcggacagg ccgaccagag tgaagaccga
     4141 cagcgcgaat ggcgcgagca gcgagatgtc cagcagcgcc agccgttcgg gaattagctt
     4201 gggccggatc gcgcccaggt gcagcttgga ggccagccgc gacgacacga tcccgccgag
     4261 cgcgcctgcg tcctccagga acggcgggat cagcaccagc agcgccggca gggccaggaa
     4321 ccgatcgcgg cgatgctcga tgaccgcgcc ggcgatcagc atcacgacac cggtcagcat
     4381 cagcatcacc acgctttcgc gcatgatccg ccgcgcggcg ggaagcttgg tgcgcagccc
     4441 catcgcgagc gctgccagcg acacggcagc cgtcgcgacg ccgaccgcga ctgttacacc
     4501 ctgctgccgc gcgaggaacg acgcggcgaa cagcgacggc agcgtgatga tgtcgcccat
     4561 gaacgtgacc accggggccg ccaccgaatc cagatcccag tcacgccggt acgcggtccg
     4621 tgccagcagg atcgtcagaa ccagaatcac gccgcccgat accgttcccg cgacgaccga
     4681 gatcgccagg aaatcccaga ccggaacggt cggcagcccg agcgcggagg tcatcacgcg
     4741 cgcggcgacg gccaggaacg ccgccgaggt gatagacagc agcgtcgcgg cgtacgcgtt
     4801 ttggtacagc acgccggtcc ggttcatcga gaggcggaac aggcccgcgt ggatggaggt
     4861 gccgaggcgc gagccgagcg cgccgaagat cgatccgcgc atgccgatga cggcgggaac
     4921 caggatgagc aggccgggaa gctcctcgag ccggtgcgtc atcgctccga gcgcaagccc
     4981 ggcgagcagg tcaccgccgg acgagatcca cagcgcgacg aagccttgac gaagcgtggt
     5041 gcgctcggct cgccagtagc ggccgatctg gacgacggcc cgtgcgggag cccggaccgc
     5101 ggcggcagga gcgcgcgcga cggtgcgcgc ggcttgcgcg gggacgcgag aaagtcgccg
     5161 gcgctcctcc ggggtcggct tcttcacccg ccacctcgtc ctccttatgc cggctcgcgg
     5221 ggatgcgaac cgcggacgag tctaaccggc gggcaagaag cctccggtta gcttccgtgc
     5281 gcgggggagc gaagcggctc gcggccgccc agcgcgcggc cggcacggat cagcgcgctg
     5341 ccgatcgcgg cgatcggggc gggccggtgc tcggcgcggc ggtgctcgcg gcgggcggct
     5401 ttcttggcca gacgggtctg gtcggccatg cgccaaagct cctcgcgccg ctcgcggatc
     5461 atgtcgtact cgaagggtgg atacatggga aaccccctgc tctccggccg gttcgcccgg
     5521 cctcttgctc ataacctgca aaaagtctat gatggttagg catcaccagt caagaggttt
     5581 tgctggttag attgagggtt ggatgatgac ggtgaaagcg gacacggtcc cggcgcccac
     5641 gcgcctggtt gccgcgttcg tcaatacgct cgacctcgag agcccggcgg accggatcgg
     5701 cacgccggca gccttgcgcg cctggcttca ggagcagagc ctgctgcccg aggacgcgcc
     5761 gctgcccggc cgctacgacg tggacatggc gcaagcgatg cgcgaggacc ttcgcgacct
     5821 gctcgagtgc aactgcggcg ggccgctcgc ggaacgttgc gtcaacggga tcaaccgggt
     5881 ctccagcgct ggccgtctgg tcgtgcgcat cggcccggac ggccgtgcgg tgctcgagcc
     5941 cgaaaccgcc ggggtgtccg gggcgctcgg tgcgatcatg gccgccgtct acacggccat
     6001 gaccgacgga acctgggagc ggctcaagac ctgccgccgc gagtcgtgcc gctgggtctt
     6061 ctacgacgca tcgaagaatc gcagcggcca gtggtgctcg atgaaggtct gcggcaaccg
     6121 tgagaaggcc aggcagtatc gggagcgaca caaggcatga ccgccttcga cgttccgatg
     6181 cacggcgcgg gcggcgagcc cgtggacctg tggcgaacca tcaactcgca cggcgtcgcg
     6241 tccttgccgc cgcacgtcgt ggacgaagac gcccggacgc tcaccacggt ggtcgcgctg
     6301 gccggcacca agccgcggcg ggtacggatc gccgccggcc gcgggcgcac cgcgcgcgtg
     6361 accatcgacg ggccggcagc aggggagcgc accgtcgctc gcgtccgcgg cgcggtccgc
     6421 cacatccttg cgctcgaccg cgacctgtcg gcgttctacg cgctggtcgc cgacgatccg
     6481 gctctggcgt ggacggtgcg cggcgcggga cgcatggtgc gctcgcagac cgtgtttgaa
     6541 gaggtggtca agacgatctg cacgaccaac tgcgcgtggt ctgcgaccga gcgcatggtt
     6601 ggcgcgctcg tcgagcatct gggtgagccg gcggcgggcg tcaagcccgg accgggcccg
     6661 gcgggacgag ccttccccac gcccgcggcg atggccgaag ccgacgacga cttctatcgc
     6721 ggcgtcgtgc gcgcgggata ccgcggccgg tactttcggg cgctggcccg cgacgtcgcc
     6781 gacggcacgc tggacctcga ggcgctcgcc acggacgacg agacatcgga tgaggacgtc
     6841 tacaagcagc tgatcgcgct tcccggcgtc ggaccgtacg cggccgcgca catcatgcag
     6901 atgatcggcc ggcactcgcg tccgatcctg gattcgtgga cacggcccac gtacgcacgg
     6961 ctgaccggca agaagtccgt caccgataag gcgatccttc gccgcttcgc ccgctaccgc
     7021 gagcacgccg gccttgcctt ctggctgttc ctgacccagg actgggtcgc cgagtagcta
     7081 gcgcaccgtc accgggacgg tgtggatcag gttgttgccg aagccgccgt aattccacgg
     7141 cgcttcgtcc accggctggg tgtcgccgct ctcgtcggtc gctcggcacg acagcacgtg
     7201 ctcgcccggc tcggcgatcc attcggcgct ccagcctcgc caggcgaagt catcgatcgg
     7261 ctcgtcgagc ttcgcctcgg tccacgcgcc gtccacgccc agctcgacgc gtacgatcgg
     7321 accgctgccc gaccaggcgc ggccgcggat catcaccggg ccggcatcta cgatccgcgt
     7381 gcgcgtgacg aagtctggga agcccggtgg catcagcaaa gatcgcacgc ggatgcgggt
     7441 gacctgcgcg ccgggatcct cgagcgactg ccggtagacg tacgcgatcg tgttttgaaa
//
LOCUS       DATLFO010000055         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_100986, whole genome shotgun sequence.
ACCESSION   DATLFO010000055 DATLFO010000000
VERSION     DATLFO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100986"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VM841_01920"
     CDS             complement(<1..597)
                     /locus_tag="VM841_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88970.1"
                     /translation="MEVEVLASGRCLLGHPVSTGPASEEPDRDAAAPPSFPAPRDLKP
                     RRRARKLPDPPAADDLPRSIRTAPTPERTLRPVPPPAEPVPAPPAPPAASAAIEPSVA
                     SLAPAEDGETVAPRISRPWAPVEPDGPSGPVGDLDPWLKKPEAQAPAQPADPPKPAPP
                     AAFAPPPRVAPRTFAPQRSDAKPEAARDRALTAGLCSQA"
     gene            770..2248
                     /gene="glpK"
                     /locus_tag="VM841_01925"
     CDS             770..2248
                     /gene="glpK"
                     /locus_tag="VM841_01925"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HVL88971.1"
                     /translation="MARILAIDGGTTGVTALIVGDDASVVAKGYREFPQHFPRPGWVE
                     HDAGQIWEATQAAVADALAQAGIEASGLAAIGITNQRETTVLWERSTLKPVSNAIVWQ
                     DRRTARRCDELRDREPAIRARTGLVLDAYFSATKIHWMMENVDGVRAAARRGDLAFGT
                     IDSWLVAKLTGGAVHATDPSNASRTLLYNIYERDWDDELLDIFGVPRSLLPEVLPSSG
                     RFGTTDPGSFFGAAIPVAGIAGDQQSALFGQACFEPGMTKNTYGTGSFVLMNTGTTAP
                     PPPDGLLVTVAWGLGGETFYAMEGSIFVTGAAIQWLRDGLGIIESASETGPLAESVPD
                     NGGVYFVPALTGLGSPWWDPYARGTIVGITRGSTRAHLARAAVEAMAYQTRDVVETMM
                     AKGGVELSELRVDGGASVMDLLCRFQADQLGVVVRRPASFETTGLGAAFLAGLAEGVW
                     ASTADIGSLWRVDAEFMPRDPDPRGYAGWQRAVERARDWARD"
     gene            2298..>2644
                     /locus_tag="VM841_01930"
     CDS             2298..>2644
                     /locus_tag="VM841_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88972.1"
                     /translation="MTRGRKVLVALFAVSLALPVNGSAGPPPPNGVPVPTMYYNGPGF
                     ATVWNGRVVAVARQGSRHWDDPSGRIGLYEFEGGKMQWPPAVLRDTHLDDRNPAGGVT
                     PTGALVYFYARLE"
BASE COUNT          352 a          941 c          979 g          372 t
ORIGIN      
        1 agcctgcgag caaagccccg ccgtcagagc ccgatcgcgc gccgcctcgg gcttcgcgtc
       61 ggaccgctgg ggcgcgaacg tgcggggcgc gacccgaggc ggaggcgcga atgcggccgg
      121 cggtgccggc ttcggcggat ccgccggctg cgccggcgct tgcgcctccg gcttcttcag
      181 ccacgggtcg agatcgccga cgggccccga cggaccgtcg ggctcgacgg gcgcccacgg
      241 ccgcgagatt ctcggcgcga cggtctcgcc gtcctcggcc ggcgcgagcg acgccacgga
      301 cggctcgatc gcggcggacg cggcgggcgg cgcgggcggc gcgggaacgg gctccgcggg
      361 cggcggcacc ggccgcagcg tgcgctcggg cgtgggagcg gtacggatgc ttctcggcag
      421 atcatccgcc gccggcgggt cgggcagctt tcgcgcgcga cgccgcggct tcaggtcgcg
      481 cggcgcggga aacgacggcg gcgcggccgc atcgcggtcc ggctcctcgc tcgccggtcc
      541 ggtcgagacc gggtgcccga gcaagcagcg gccggaggcc aggacctcga cctccatgcc
      601 gcaccaatcg cagcgggctc tcatcgctaa cgcgctagtc tcgcgcaaca cggcctccga
      661 tgcacgggct cttcatgctt cttcgactcc gcaggacccg gatgcttgcg ctttcgccgc
      721 ctctggcggg tttgcgtccc gcgcgcggcc tgcgacagta tcggcgccca tggctcgaat
      781 cctggctatc gacggcggca cgaccggtgt gaccgcgctg atcgtcgggg acgacgcgag
      841 cgtcgtggcc aagggatacc gcgagttccc gcagcatttt ccccgtccgg gctgggtcga
      901 gcacgacgcc ggccagatct gggaagcgac gcaagccgcc gtcgcggatg cgctggcgca
      961 ggccgggatc gaggcgagcg gcctcgccgc gatcggcatc acgaaccagc gcgagaccac
     1021 ggtgctgtgg gagcgatcga ccctgaagcc ggtgtcgaac gcgatcgtgt ggcaggaccg
     1081 gcgcacggca cgccgctgcg acgagcttcg cgaccgcgag cccgcgatcc gcgcgcgcac
     1141 cgggttggtg ctggacgcgt acttcagcgc caccaagatt cattggatga tggagaacgt
     1201 ggacggcgtg cgcgccgcgg cgcgccgcgg cgacctggcc ttcggcacga tcgactcgtg
     1261 gctggtcgcc aagctcaccg gcggcgccgt gcacgcgacc gatccgtcca acgcctcgcg
     1321 cacgctgctg tacaacatct acgagcgcga ttgggacgac gagctgctcg acatcttcgg
     1381 cgtgccgcgc agcctgctgc ccgaggtgct gccgtcctcg ggccggttcg gcaccaccga
     1441 cccgggttcg ttcttcgggg cggccatccc ggtcgcgggc atcgccggcg atcagcaaag
     1501 cgccctgttc ggtcaggcct gcttcgagcc gggcatgacc aagaacacct acggcacggg
     1561 atcgttcgtg ctgatgaaca cgggcaccac cgctcccccg ccgcccgacg ggctcctggt
     1621 caccgtggcg tgggggctgg gcggcgagac cttctacgcg atggaaggct cgatcttcgt
     1681 gaccggcgcg gcgatccagt ggctgcgcga cgggctcggc atcatcgaaa gcgcgagcga
     1741 gaccggcccg ctggccgaat cggtgcccga caacggtggc gtctatttcg tccccgccct
     1801 gaccgggctc ggctcgccgt ggtgggatcc gtacgcgcgc ggcacgatcg tgggcatcac
     1861 ccgcggcagc acacgcgcgc atctggcgcg agccgccgtc gaagcgatgg cgtaccagac
     1921 ccgcgacgtc gtcgagacga tgatggcgaa gggcggggtc gagctcagcg agcttcgcgt
     1981 ggacggcggc gcgagcgtga tggatctgct gtgccgcttc caggccgatc agctcggcgt
     2041 ggtcgtgcgg cgcccggcca gcttcgagac caccggcctg ggcgcggcgt tcctggccgg
     2101 tctggccgag ggcgtgtggg cctccacggc cgacatcggc tcgctgtggc gggtggacgc
     2161 cgagttcatg ccgcgcgatc ccgacccgcg cggctatgcc ggatggcagc gcgccgtcga
     2221 gcgcgctcgc gactgggcgc gcgactagcg cgacgagagg ggttgcccct gccgccgcgg
     2281 gccggaacaa tgggccgatg actcgcggac ggaaagtctt ggttgccctg tttgcggtct
     2341 cgttggcgtt gcccgtgaac gggtccgccg gaccgccgcc gccgaacggc gttcccgtgc
     2401 cgacgatgta ctacaacggt cccggcttcg cgaccgtctg gaacggccgg gtcgtcgcgg
     2461 tcgcccgcca gggaagccgc cactgggatg acccctccgg ccgcatcggg ctctacgagt
     2521 tcgagggcgg caagatgcag tggccaccgg cggtgctgcg cgacacccac ctcgacgacc
     2581 gcaacccggc gggcggcgtc acgcccaccg gcgcgctcgt gtacttctac gcccggctgg
     2641 agga
//
LOCUS       DATLFO010000056         3907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_41603, whole genome shotgun sequence.
ACCESSION   DATLFO010000056 DATLFO010000000
VERSION     DATLFO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41603"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VM841_01935"
     CDS             <1..222
                     /locus_tag="VM841_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVL88973.1"
                     /translation="GARIQAVVPGGPAEAAGLRAGDVVVEIEGNAIKSAEELVAVVAR
                     FDVGQTVTVVFERDGKRQTARATLTDRPR"
     gene            310..738
                     /locus_tag="VM841_01940"
     CDS             310..738
                     /locus_tag="VM841_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HVL88974.1"
                     /translation="MGELIVIVIVALMVFGPKRLPEMMRSAGKAFRMFQTESQKAVAE
                     LREAVDTSDIQNDLRKIVMDEPDELARKAEERAAAAAAANAAAAAASTPPVLDPIPPH
                     AAKKTAAKKTAAKKTAAKKTAAAAPLAAQPPGGYPVTEDT"
     gene            735..1316
                     /locus_tag="VM841_01945"
     CDS             735..1316
                     /locus_tag="VM841_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVL88975.1"
                     /translation="MSRTKRPGLAKPLIRLGAAAALGSLSRARLAEADKQAFRALRSR
                     RSETLDRVLPIATDFGSVYAVAGVATSLILLGRPRLAVRATGAALTAWAVAQGAKKVY
                     VRPRPYDDGVIEVLVRRPAGTSYPSGHPAVAAALARVLAPEANKAVGKLIERLPSFVA
                     ASRVYVGVHYPSDVIGGRLVGEAVADVWRRYLP"
     gene            complement(1321..2436)
                     /locus_tag="VM841_01950"
     CDS             complement(1321..2436)
                     /locus_tag="VM841_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HVL88976.1"
                     /translation="MATKEEVLQALATVNDPEIGRPITELDMVKNVTVDDDIVVVEIL
                     LTVAGCPLKDKITKDVTAAVAPLSDVSKVHVEMGVMTDEQRQAMVSRLRSGQGEQGAI
                     KEIPFWGQDRRTKAILVSSGKGGVGKSSVTANLAVALAQQGHNVGVIDCDVWGFSIPR
                     MMGVSGRPTAFDGMILPLEAHGVKVISMGFFIPDDQPVIWRGPMLHKAINQFLADVYW
                     GDLDFVLLDLPPGTGDVSISLAQFLPGADMLIVTTPQEAAQKVAARAGRMTEQVPLKI
                     IGVIENMSYFVCPDCDSTHRIFGEGGGEQLAKTLGTELLGEVPIQETLREGADIGTPI
                     VALKPESPAAQALQQVAAKIAANKPSIKGKRLPLFSK"
     gene            2778..>3907
                     /locus_tag="VM841_01955"
     CDS             2778..>3907
                     /locus_tag="VM841_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HVL88977.1"
                     /translation="MDVVGIGLAGGRGERARPLTVKAPGYLRAKAAMSFLGQRLVRLI
                     IGILREQGITDYYVVAHGKENRYQIKTLIDHGEPLGVNVKYSRVRFDALNTGSADTTI
                     RNLRYWDLHGTGLIFPTDSVIDFDLKEMVEAHKSSGAVITIGAMVREPMEVAGKYGVM
                     LTDPQSTILEFVEKPTLQEIHEAFPVPSEDAFNRLPLLTNSGFYLVDLDALNEIGDHP
                     DVLEMAESRLDFGLDLLPWVVGKGFKVNAAPIGRIGDLGNIPDYVETMVDALDGRFAS
                     VERQLGEPFDEERNIWIDPTSLHMRDTVSGTTLEEKLNRGEVVLGPGVRIGKYSEIRT
                     GTRISYSNVDDGAEVHEGSVITRSAIRDGAIISRGARITDAYV"
BASE COUNT          640 a         1349 c         1311 g          607 t
ORIGIN      
        1 ggcgcgcgga tccaagcggt cgtgcccggc gggcccgcgg aggccgccgg cctgcgcgcg
       61 ggcgacgtcg tggtcgagat cgaaggcaac gcgatcaagt ccgccgagga gctcgtggcc
      121 gtcgtcgcgc gattcgatgt cgggcagacc gtgaccgtgg tgttcgaacg ggacggcaag
      181 cggcagaccg cgcgcgccac gctgacggac cggccacgat agggccccgg caggcggcct
      241 ccggcccggt cgccgataat agggcgtcca tcgaacagcc aggaggctct tctgctgccc
      301 aacgtcggaa tcggcgagct gatcgtcatc gtgatcgtcg cgctgatggt gttcggtccg
      361 aagcgactgc ccgagatgat gcgctcggcc ggcaaggcct tccgcatgtt ccagaccgag
      421 agccagaaag ccgtcgccga gctccgcgaa gccgtggaca ccagcgacat ccagaatgat
      481 cttcgcaaga tcgtgatgga tgagcccgac gagctcgccc gcaaggccga ggagcgtgcc
      541 gccgccgctg cggccgccaa cgccgctgcc gctgcggcct ccacgccgcc cgtgctcgac
      601 ccgattccgc cgcacgcggc gaagaagacg gcggcgaaga agacggcggc gaagaagacg
      661 gcagcgaaga agacggcggc cgcggcgccg ctcgcggcgc agccccccgg cggctatccc
      721 gtcacggagg acacatgagc cgaacgaagc gacccgggct cgccaagccg ctgatacgcc
      781 tgggtgcggc tgccgcgctc ggctccctgt cgcgtgcccg gctcgccgaa gccgacaagc
      841 aagcgtttcg ggcccttcgt tcccgccgca gcgagacgct cgaccgcgtg ctcccgatcg
      901 ccaccgactt cggttcggtg tacgcggtcg cgggcgtggc gacctcgctg atcctcctcg
      961 gccggcccag gctcgccgtc agggccacag gggccgcgct gaccgcgtgg gcggtcgctc
     1021 aaggcgccaa gaaggtctac gtccggccgc gaccatacga cgacggcgtg atcgaggtcc
     1081 tggtccgccg gccggcggga acctcgtatc ccagcgggca tccggccgtg gccgcggcgc
     1141 tcgcgcgcgt gctggcccct gaagccaaca aagccgtcgg aaagctgatc gagcggctgc
     1201 cctcgttcgt ggctgcctcc cgcgtgtacg tgggcgtgca ctacccctcg gacgtgatcg
     1261 gcggacgcct cgtcggtgag gccgtcgccg acgtgtggcg gcgctacctg ccctagcagc
     1321 tcacttcgag aacagcggca ggcgcttgcc cttgatcgat ggcttgttcg ccgcgatctt
     1381 ggcggcgacc tgctgcagcg cctgcgcggc cggcgactcg ggcttgagcg ccacgatcgg
     1441 cgtgccgatg tcggcgccct cgcgcagcgt ctcctgaatc gggacctcgc ccagcagctc
     1501 ggttccgagg gtcttggcga gctgctcgcc gccgccctcg ccgaagatgc ggtgcgtgga
     1561 gtcgcagtcg gggcacacga agtagctcat gttttcgatc acgccgatga tcttgagcgg
     1621 cacctgctcg gtcatgcgcc ccgcgcgcgc ggcgaccttc tgcgcggcct cctgcggcgt
     1681 ggtcacgatc agcatgtcgg cgcccggcag gaactgcgcg agcgagatgc tgacgtcgcc
     1741 ggtgcccggc ggcaggtcga gcagcacgaa gtccaggtcg ccccaataga cgtcggcgag
     1801 gaactggttg atcgccttgt gcagcatcgg gccgcgccag atcacgggct ggtcgtccgg
     1861 gatgaagaag cccatcgaga tgaccttcac gccgtgcgcc tcgagcggaa ggatcatgcc
     1921 gtcgaaggcg gtcggccggc ccgacacccc catcatgcgg gggatcgaga agccccacac
     1981 gtcgcagtcg atgacgccga cgttgtggcc ctgctgcgcg agggcgaccg cgaggttggc
     2041 ggtgacgctg gacttgccca cgcccccctt gcccgacgag acaaggatcg ccttcgtccg
     2101 gcggtcctga ccccagaacg ggatctcttt gatcgcgccc tgctcgcctt gccccgagcg
     2161 cagccggctg accatggcct ggcgctgctc gtcggtcatc acgcccatct cgacgtgaac
     2221 cttggacaca tccgacaggg gagcgacggc agcggtgacg tccttggtga tcttgtcctt
     2281 gagcggacag ccggcgaccg tcagcaggat ctcgaccacg acgatgtcgt cgtcgacggt
     2341 cacgttcttg accatgtcga gctcggtgat cggccggccg atctcggggt cgttcacggt
     2401 tgcgagcgcc tgcagcactt cttccttggt tgccacgatt tgtccgccct tcggttccga
     2461 ttcgagtacc gccaacaggc tagcacggca cgcaacacgg ttttttccgc gtcgtggggc
     2521 gatttctcca tcgcacaacg tcgatggacg cgcgataatg gggtcgaacc tcagacctcg
     2581 gtgcccccgg gagcggagac caagccgacc agctccccca ccgctcctcg ggggcccact
     2641 tatccgctcc gccgtcatgc ctcgaacgag tgaagaatca ctcctgcggg ggagaggatc
     2701 ggccaaacgc ggtaccatgg cgcggggcgg ctcctcgtcc catccccgtg caccaccatc
     2761 gaggaggcgg tctgactttg gacgtcgtcg gaatcggtct ggccggcggc cgcggcgaac
     2821 gtgcccggcc cctgaccgtg aaggcgcccg gttatctgcg ggcgaaggcg gccatgtcct
     2881 tcctggggca gcgactggtt cgcttgatca tcggaatcct gcgcgagcaa ggcatcaccg
     2941 actactacgt ggtcgcgcac ggaaaagaga accgctacca gatcaagacg ctcatcgacc
     3001 acggtgagcc gctcggcgtc aacgtcaagt actcgcgcgt tcggttcgac gcgttgaaca
     3061 cgggctcggc ggacaccacg atccgcaacc tccgctactg ggacttgcac ggcacgggcc
     3121 tgatctttcc gaccgactcg gtgatcgact tcgatctgaa ggagatggtc gaggcgcaca
     3181 agtcctccgg cgcggtcatt accatcggcg cgatggtccg cgagccgatg gaggtcgccg
     3241 gcaagtacgg cgtcatgctc accgacccgc agagcacgat cctcgagttc gtcgagaagc
     3301 ccacgctgca agaaatccac gaagcgtttc ctgtgccgag cgaggacgcg ttcaaccggc
     3361 tgccgctctt gaccaactcc ggcttctatt tggtcgacct cgacgcgctc aacgagatcg
     3421 gcgaccaccc ggacgtgctc gagatggccg agagccggct cgacttcggc ctcgacctgc
     3481 tcccgtgggt cgtgggcaaa ggcttcaagg tcaacgccgc cccgatcggc cggatcgggg
     3541 acctcggcaa catccccgac tacgtcgaga cgatggtgga cgccctcgac ggccgcttcg
     3601 cctcggtcga gcggcagctc ggcgagccgt tcgacgagga gcgcaacatc tggatcgatc
     3661 ccacgtcgct gcacatgcgc gacacggtga gcggcaccac gctggaggag aagctcaacc
     3721 gcggcgaggt cgtgctcggc ccgggcgtgc gcatcggcaa gtacagcgag atccgcacgg
     3781 gaacgcgaat ctcgtactcg aacgtcgacg acggcgccga ggtgcacgaa ggctcggtca
     3841 tcacgcgcag cgcgatccgc gacggcgcga tcatcagccg cggggcgcgc atcaccgacg
     3901 cctacgt
//
LOCUS       DATLFO010000057         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_91612, whole genome shotgun sequence.
ACCESSION   DATLFO010000057 DATLFO010000000
VERSION     DATLFO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91612"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VM841_01960"
     CDS             <1..924
                     /locus_tag="VM841_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88978.1"
                     /translation="GVSSAYASATRDAAQAIIADLSLGVATARAVASTSSVEWKGSGL
                     IATTSVQIDELRIASADPAVPMLTISSLRSVATASSDGRITGGSLELSGVRVALPGLP
                     ATMPCSAAKPCAATIGRDGIHVTDVAVPDQMRDVLDQALSLIAVRIPAAPAEVAGQTA
                     AARGDAAGPGVTIASDRRRVSVSGLEIDVSADNSLEGRTVVSLRIGAASAAAIAAWVP
                     MIDPSDDEQVDPAFVGGPRGGNPAPPPHGDDEYEPPAPRTGGPAVSRPVASSQMPESR
                     PIPPAVALLAFAAILAGAAGLVAFGIWETAP"
     gene            921..1391
                     /locus_tag="VM841_01965"
     CDS             921..1391
                     /locus_tag="VM841_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88979.1"
                     /translation="MSRLPREFAKAFWQLIAGGVCIATGIAAIFLGWFGAAHTRDVTD
                     QLPYLISGGLFGLALVVLGSALYFSYFVAQVHHASRRQQRALEQMLMSAGARVGTSDG
                     EEGLVVTVPGGASYHRSGCPLAGGKDASPKPVAEASEQGYKPCAICEPSAAHAN"
     gene            1473..>2336
                     /locus_tag="VM841_01970"
     CDS             1473..>2336
                     /locus_tag="VM841_01970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HVL88980.1"
                     /translation="MSEIRDFPRRFAAASRDKTATLADAVAAIVRPGDALHLGATHIR
                     GAAAAWEILKQFRGTDPGFEIQSLSMSTALAPLVHERLASRIVTTWAGDAYYSPGPNP
                     VFTRSHADGVDWRHWSIMTFPQRLQAAARGLAWTTTKSLIGSDLAKENAGDVREIDDG
                     LLLIRALTPDVSCFHAPAADAAGNVLVPPPLLENVWGALAARRGAIVTVEKIVDEQTI
                     REHSSMVRIPRHVVAAVCEVPFGAHPGGVFARDVAAVTGYGEDYPFWHEMRAACRDDV
                     PRDPHHRGVLPD"
BASE COUNT          311 a          817 c          839 g          369 t
ORIGIN      
        1 ggtgtgtcga gcgcgtacgc gtcggcgacg cgcgacgcgg cgcaagcgat tatcgcggac
       61 ctgtcgctgg gcgtcgccac cgcacgtgcg gtcgcctcga cctcgagcgt cgaatggaaa
      121 ggctcgggcc ttatcgcgac cacgagtgtg cagatcgacg agcttcggat cgcctccgcc
      181 gatccggcgg tgccgatgct cacgatctcg tcgctgcgct cggtcgcgac cgcctcgtcg
      241 gacggacgga tcaccggcgg atcgctcgag ctgtccggtg tgcgcgtcgc gctgcccggg
      301 ctgccggcga cgatgccgtg ctcggccgcc aagccgtgcg ccgccacgat cggccgcgac
      361 ggcatccacg tcaccgacgt tgcggtgccc gatcagatgc gcgatgtgct cgatcaggcg
      421 ctgtcgctga tcgcggtgcg catccccgcc gcgcccgcgg aggtggcggg gcagaccgcg
      481 gccgcgcgcg gcgacgcggc gggtcccgga gtcacgatcg cctcggatcg ccggcgcgtg
      541 tcggtgtcgg gcctcgagat cgatgtgagc gccgacaact cgctggaggg ccgcaccgtc
      601 gtgtcgttgc gcatcggcgc ggcatcggcc gccgcgatcg cagcgtgggt gccgatgatc
      661 gaccccagcg acgatgagca ggtcgatccc gccttcgtcg gcgggccgcg aggcggaaac
      721 cccgcgccgc cgccgcacgg cgacgacgag tacgagccgc ccgcgccccg gacgggcggg
      781 ccggccgtgt cgcggccggt tgcgtcgtcg cagatgcccg agtcgcggcc gatcccgccg
      841 gcggtggcgc tgctggcgtt cgcggcgatc cttgccggtg cggcgggttt ggtcgcgttc
      901 ggcatctggg agaccgcgcc gtgagccgcc tcccgcgcga gttcgccaaa gccttctggc
      961 agctgatcgc cggcggcgtt tgcatcgcga ccggtatcgc cgcgatcttc ctcggctggt
     1021 tcggcgccgc gcacacacgc gacgtcaccg accagctgcc gtatctgatc tcgggcggtc
     1081 tgttcggcct ggcgctggtg gtgctcggca gcgcgctgta cttctcgtac ttcgtcgcgc
     1141 aggtgcatca cgcgtcgcgg cggcagcagc gcgcgctcga gcagatgctg atgagcgccg
     1201 gtgcgcgcgt cggcacttcg gatggcgagg agggcctggt cgtgaccgtg cccggtggcg
     1261 cgtcgtatca ccggtccggc tgtccgctcg ccggcggcaa agatgcgagc cccaagccgg
     1321 tcgccgaggc atccgagcag ggctacaagc cgtgcgccat ctgcgagccg agcgccgctc
     1381 acgccaacta gcggcctttg ttggtcagat gagttcgcta ttcggagtca tatgaccaac
     1441 aaaggtccga agacccatta gcatggacgg ccgtgagcga gattcgcgac tttccgaggc
     1501 gctttgcggc tgcatcgcgc gacaagaccg cgacgctcgc cgacgcggtt gcggcgatcg
     1561 tgcggccggg cgatgcgctg caccttggcg cgacgcacat tcgcggggcg gcggcggcgt
     1621 gggagatcct caagcagttc cgcggcaccg atcccgggtt cgagatccag tcgctgtcga
     1681 tgtctacggc gctcgctccg ctcgtgcacg aaaggctcgc gagccggatc gtgaccacgt
     1741 gggccggcga cgcgtactac tcgcccggcc ccaacccggt cttcacgcgc tcgcacgccg
     1801 atggcgtgga ctggcggcac tggtcgatca tgacgttccc gcagcggctg caagccgccg
     1861 ctcgcggcct tgcctggacg acgacgaaat ccttgatcgg ctcggacctt gcgaaagaga
     1921 acgccggcga cgtccgcgag atcgacgacg ggctgctgct gattcgcgcg ctcacgcccg
     1981 acgtgtcgtg cttccatgcg cccgcggccg atgcggccgg caacgtgctc gtcccgccgc
     2041 cgctgctgga aaacgtctgg ggcgcgctcg ccgcgcggcg cggcgcgatc gttaccgtcg
     2101 aaaagatcgt ggacgagcaa acaatccggg agcactcctc gatggtgagg atcccgcggc
     2161 acgtcgtcgc ggccgtctgc gaggtgccgt tcggcgcgca tcccggcggc gtcttcgcgc
     2221 gcgacgtcgc agccgtcacc ggctacggcg aggactatcc gttctggcac gagatgcgcg
     2281 ccgcctgccg cgacgacgtg ccgcgggatc ctcaccatcg aggagtgctc ccggat
//
LOCUS       DATLFO010000058        15968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_41626, whole genome shotgun sequence.
ACCESSION   DATLFO010000058 DATLFO010000000
VERSION     DATLFO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41626"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VM841_01975"
     CDS             complement(<1..777)
                     /locus_tag="VM841_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HVL88981.1"
                     /translation="MADEVLQLTKKDFESDQEVRWCPGCGDYAILSAMQAAMPAMNIP
                     REKVVFVSGIGCSSRFPYYMNTYGMHSIHGRAPAVATGIATAAPDLSVWVITGDGDGL
                     SIGGNHLIHALRRNVNLKIILFNNQIYGLTKGQYSPTSELGKVTKSTPFGSLDQPFNP
                     IALALGSEATFVARSIDVDRKHLTEVLHAAAVHKGSAFVEIYQNCNIFNDGAFEDLTS
                     RDKKMQNQIRLQHGEPVRFGENNEKGVRMTGNAQLEIVDVD"
     gene            complement(783..2618)
                     /locus_tag="VM841_01980"
     CDS             complement(783..2618)
                     /locus_tag="VM841_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HVL88982.1"
                     /translation="MSKETISLDRVVIRFAGDSGDGMQLTGDRFTNVSAILGNDLSTL
                     PDFPAEIRAPAGSLAGVSGFQIHFSSEDILTPGDQPNVLVAMNPAALKTNLKDLQTGG
                     FLIVNEDAFTERNLEKAGYDKNPLEDGSLDGYQLIKVKMTQMTVDATKGIEGVTKKDS
                     ERAKNMFALGLLSWMYTRPVDPTTEWIQKRFAKAPPIMEANLAAFKAGHAFGETAELF
                     ASHYEVKPAVLKKGTYRNITGNTALAWGLIAAADRASLPLYYASYPITPASDVLHELS
                     KHKNFGVATLQAEDEIAAACMAVGAAFAGQLAVTATAGPGIDLKSETLGLAVILELPM
                     IICDVQRGGPSTGLPTKIEQSDLLAAMYNRHGEAPMPIVAAQSPSDCFAAAIEAARIA
                     IKYRTPVFLLSDSFLANSAEPWRIPNVKDLPVIENTFATEPNKGGEFWPYLRDENLAR
                     PWAVPGTPGLEHRIGGLEKQDGTGNVSYDPANHEYMTRVRAAKVAKIAEDIDPLHVDE
                     QGGEELLVVGWGSTWGSIQAAVRRVRAQGHKVAHAHLRHLNPMPKNTGEVLKRFSRVL
                     VPELNNGQLVRMLRAEYLVDAKSYTKIQGQPFLAAELETEILKRL"
     gene            2894..4045
                     /locus_tag="VM841_01985"
     CDS             2894..4045
                     /locus_tag="VM841_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HVL88983.1"
                     /translation="MREIYLDHASTTPPHPAALEAMSKAATELWGDPSRLYRAARVAR
                     IALDEARAAVASVIGGRPEEIVFTSGGTESCNLGIVAGARAAAAARKPKRIVVPAVEH
                     TAVLESAQALDGFEIVRVGVDEHGRVDLDTLRDECARGAGLVSIQAANHEVGTIQPLA
                     DAALLAKQAGALIHTDACMAVGQIPVDVKALGVDLLSGSGHKAYGPRGTGFLWARTGV
                     RVRPVMVGDDRERRRRAGLENLPAIAGMAAALTARAGEIDAEMPRLRALTDRIRTQLP
                     ALIEDVQIHGHPTERLPGLVAFSLLYLEGEALLLMLDGKGIAVHSGSSCVSSAQEPSH
                     VLAAMGALTHGSVRVSLGRGSTDEDVNAFLKELPPIVANLREIARTGSA"
     gene            4042..4278
                     /locus_tag="VM841_01990"
     CDS             4042..4278
                     /locus_tag="VM841_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HVL88984.1"
                     /translation="MSEQPAATLDVLGLPCPMPVVRLSKTIGTVPVGAIVEVLSDDEA
                     AKIDIPVWCRMKKHEYLGVKATERGPAHLVRRML"
     gene            4287..4433
                     /locus_tag="VM841_01995"
     CDS             4287..4433
                     /locus_tag="VM841_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88985.1"
                     /translation="MEAVYGIWVLAGFVFFFWFVATMHGIAKDLHAIREAVAPEPSTP
                     RSKA"
     gene            4502..5644
                     /locus_tag="VM841_02000"
     CDS             4502..5644
                     /locus_tag="VM841_02000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVL88986.1"
                     /translation="MSAIPRAATRALRSPAGHTEPIGGRNLAVESLRGIAALAVVGYH
                     LAAVTEWPASFPPRLLLSNGDAGVTLFFVLSGFLLWRPFAKAAIEAGPMPGLRQFFTN
                     RALRIVPAYWVALAVTIVALGATARGGAWRYLIFAQNYSRESFFGVISPAWSLAVEAV
                     FYLVLPVAGFAAVRLARSAPSPIRAVYGVVGAWFAAGLAYKLYALGVQDVDPTDPLFL
                     FNFPAKAHLFAGGMLLATWHARDRMRSPLGSSGRSALIGSAIVVAAVGIRYRSAATAA
                     FFDTLTAAGFTLIVAAVAYGQPRLSRLSRSLHWRPLIAAGVVSYSIYLWHHPLIERLH
                     EAGVLRSEFAWLPLNIVVVLAITIAVAAASYLLIERPFLKLRRRWS"
     gene            5691..7265
                     /locus_tag="VM841_02005"
     CDS             5691..7265
                     /locus_tag="VM841_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88987.1"
                     /translation="MKPTFRRVAAWLAACAAALSTLSAGATIPIDDPCARYRALGGNL
                     TAEVSDSAPGSGPLRVFAMQHKQLVDYVETYETYAAKMDCMMRDYVAPHLDRSKTNLI
                     VFNEDIGLITLGTGSRGAQARALAASPMPGSTPETLDATGAPLGGAAALGSVAATYQR
                     QMLDVERRFPGTDPRKAILLAATDTFVRGFSATFSRLARTYGVWMVSSNNQGEFDYST
                     DPVDVATWADPDLAADYAAGRIKGVFVPRSEKVWNNAFLWAPYDLRPPKPGASADPAS
                     PDYDPDTNLLFTNRKTPLTEIEKAFFALDEGDMSVSNTGPVEIPGLAGFKLGFAISLP
                     AFRFGNDFGEPFFGDPCASANSWMRCLHERGVNTIIQPEANPGPWARYGADGGNFQSL
                     TWGASTLRMVTDPTVPNFRYVVCPHMTGNLIDLPFDGQSAILERGRTGTARAYVGAKD
                     FIPETDSAWTMPYAGLKPEFLALAAWVIDDDHSLSPIENRRRLHNRASELMAGSRSEH
                     SNAYLETAIFADLATP"
     gene            7303..8838
                     /locus_tag="VM841_02010"
     CDS             7303..8838
                     /locus_tag="VM841_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVL88988.1"
                     /translation="MMQPGETLEREAAQLYYDHAYAAAAQRYERAFVAYRGENNHLAA
                     GLAARAIGWITGNVLGDWAVQNGWFARSLAEFEKAGDPVARGWALTVAADLEMDYPKR
                     APLYADAVAVAREHGNVDLEYEAHAYLGLLYVMEGRFEEGLLYLDEGLAAVCSGEITD
                     IVVFDGIFCGFFWACEMLNDVARADQWMRAAADIMHGRQIVAAFCRAHYGAILTAAGR
                     WKEAEHELLQASRHFQTSSTPRRSAAMIRLADLRVRQGRLEEAAQLLRGAEQDPDAAR
                     PLAALYFARGETARARDLLERATQGEGVPALIGPLLALLVEVSLAEGNVEQATHAAER
                     LESLSSVHHVPYLRAAAALAEGRVCIATDTGDARARLHEALEGFGRAQLPMELAMAHL
                     HMARAQAERSPEAAIAEAKAALEDFERLEAARHADEAAAVLRGLGAPARTGPKGGGTL
                     TRRETEVLRLIGSGLSNGEIGDRLYITRKTVEHHVGSLLMKLGLRNRAEAAAYITREE
                     SGH"
     gene            8914..10113
                     /locus_tag="VM841_02015"
     CDS             8914..10113
                     /locus_tag="VM841_02015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL88989.1"
                     /translation="MKTHDVIVVGARCAGSPTAMLLARKGYDVLLVDRATFPSDTLST
                     HLLHAPGVDALRRWGLLDRLIATGCPPIESYKFDFGPLVIEGAPGTAYAPRRTVLDEL
                     LVNAAAEAGAEVREGFSVEEVLIEDGRAVGIRGHGKDWASVTQRARIVVGADGRYSRV
                     AKEVKPERYNEIGPILSGYYAYWSGLPVDGFETYVRPDRSWAVWPTHDGLTLVIAGWP
                     YAEFEANKSDIEGNYLKMFELAPEFDARIRGAKRESRFAGTAVENYFCKPYGPGWALV
                     GDAGYNKDFVTAQGMSDAFRDAELCVTALDESFGGARSFDDAMETYHTTRDERSIGIF
                     EFTTMLATLEPPPPETQQLLGAMVGNQDAIDGFARVVAGVTPPSEFFSEANVGRIFEQ
                     ASAIATA"
     gene            complement(10308..10715)
                     /locus_tag="VM841_02020"
     CDS             complement(10308..10715)
                     /locus_tag="VM841_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVL88990.1"
                     /translation="MKFVDTSYWVALQVSRDRNHRDARALWGAGSLLTTTLILGETWT
                     YLNRRAGHQFAVGFLDRAQRSPALSIVHVDEKTERDAWAWLRRHDEREYSFVDATSFA
                     TMRRMKIREALAFDGDFTAAGFTEVRPGKKRRS"
     gene            complement(10712..10918)
                     /locus_tag="VM841_02025"
     CDS             complement(10712..10918)
                     /locus_tag="VM841_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVL88991.1"
                     /translation="MKRLQIYIEEELDERLAVEASREGTSKAALIRRFVAEKLRPAKA
                     DPLDEVVGWLDVEAESVDDVVYGR"
     gene            11003..11242
                     /locus_tag="VM841_02030"
     CDS             11003..11242
                     /locus_tag="VM841_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88992.1"
                     /translation="MSKMIQVRNVPDRLHRELTKRAKARGQTLTDYIQELLERDVARP
                     PAVEVFDRIEARKPVKLGRRAADLIREERDGRDAS"
     gene            11248..11640
                     /locus_tag="VM841_02035"
     CDS             11248..11640
                     /locus_tag="VM841_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVL88993.1"
                     /translation="MVSDASALIEYLLGTDAAGPVRDILRLPDVDLHVPALCDVEVVS
                     GLRRATRRRLLSDRRAAEALDDYVDLPLSRHGHRSLLARVFELRANFSAYDATYVALA
                     ERIGAELLTADERLGRAVRAQSAVATIP"
     gene            11992..13641
                     /locus_tag="VM841_02040"
     CDS             11992..13641
                     /locus_tag="VM841_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88994.1"
                     /translation="MSPIARALIVAGIAVSLVASAMVVADRNAPARRVAAADDDLLAS
                     PPEDAAVAATRFAAAIHGVPVRDGDPDDSSRYCSTDRLRVLWTAPGEGYTHGGYIEPL
                     GPEPGPDTVAVNGVVVCEGSAYAYMGFEALRDGDGWQIIPVPAMLDDDSGNDEETPGN
                     TVDPGLTAKASPPAATAAASGKVPTRSTATLTAAIETYAGYDAQDTCSPPAKPGVLAF
                     RKLVLGTYPGTRSLGISRGCGVGGRSEHKEGRAWDWGVNVNRPAERAAADALIGWLLA
                     TDEFGNRHAMARRLGIMYIVWNRTIWGSYRSDQGWRAYNGASPHTDHVHFSFAWPGAN
                     GATSFWTGAVATMPGGNGGGGSFRDAGGGGSVRGVPRDGERWWRNRTDEGTRTDIQRK
                     RTTRTEPIRWQDRSGRWWEWDRETRRWKRIDDPSATPTPTPEPEPTPTLESDPSPEPE
                     SEPKSSGTPAPSPSMSPRPSPSATSGTVRWDGSRTGGDRTGTTEERRRTTREERRIER
                     EAKRRQQREVRRNERKRKRLEQKERQRKRREQRMRTRTTNG"
     gene            13838..14239
                     /locus_tag="VM841_02045"
     CDS             13838..14239
                     /locus_tag="VM841_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL88995.1"
                     /translation="MPVITGIHHLSLTVRDGAASEEFYSRVLGFVPVLHLPDAEGRGA
                     KHVMAHPASGTVVGFTVHQANDGSAFTEFRTGLDHFALGISSHAELDEWIARLDELGV
                     EHGDVVKTPVGDYVSLRDPDNVQIELWVDPK"
     gene            14245..15024
                     /locus_tag="VM841_02050"
     CDS             14245..15024
                     /locus_tag="VM841_02050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL88996.1"
                     /translation="MATGVVVLETHLHYLRALLTRGSGSVQDRDGVLVYQSPAPLPFL
                     VNGVARTDPSVAPAAVLGAAAAAFGDRGFEVLCLDGRDDDLRAAAEAAGFQTGSPDPL
                     QATARVPDPAPIDHSAFDIRLAEYAADVDDIVTIAVDAHTVYKFPDAFFREIFRIPES
                     ILSPDIRAVIVSQQGTPVATAQAFLTGSIVYIGWVAVVVAAARRGLGWLATHEAVAAG
                     FASGAKEAVLLASPMGAPLYRTMGFTDVGTLRNAQAPPARG"
     gene            15468..>15968
                     /locus_tag="VM841_02055"
     CDS             15468..>15968
                     /locus_tag="VM841_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL88997.1"
                     /translation="MAVLLLVYNLALGLVEVALGVRAARSRRSTDALILVGASAVGAF
                     LFALVIAGGPGFRMLRLLSWGLFGHVVVSLVAAAVLTRRTRWMAAIFAACAIAGGSIG
                     VYAFRIEPFMLEVSRYRIETEKLDRPLRVAVLADFQSDEIGDYERRVIRTVAAQDADL
                     ILMPGDY"
BASE COUNT         2560 a         5508 c         5270 g         2630 t
ORIGIN      
        1 gtccacgtcc acgatctcga gctgcgcatt gccggtcatg cgcacgccct tttcgttgtt
       61 ctcgccgaag cggaccggct cgccgtgctg cagccggatc tggttctgca tcttcttgtc
      121 ccgcgaggtc aggtcctcga atgcgccgtc gttgaagatg ttgcagttct gatagatctc
      181 cacgaacgcc gagcccttgt gcaccgcggc cgcgtgcagc acctcggtca ggtgcttgcg
      241 gtccacatcg atcgaacggg ccacgaacgt cgcctcgctg cccagcgcca gcgcgatcgg
      301 gttgaacggc tggtccagcg agccgaacgg cgtggacttg gtgaccttgc ccagctccga
      361 cgtcggcgag tattggcctt tggtcagccc gtagatctgg ttgttgaaca ggatgatctt
      421 caggttcacg ttgcgccgca gcgcgtggat caggtggttg cccccgatcg acaggccgtc
      481 accgtcgccg gtgatcaccc agaccgacag gtccggcgcg gcggttgcga tgccggtcgc
      541 tacggcgggc gcgcggccgt ggatcgaatg catgccgtag gtgttcatgt agtacgggaa
      601 acgcgacgag cagccgatgc ccgacacgaa cacgaccttc tcgcgcggaa tgttcatcgc
      661 cggcatggcc gcctgcatcg ccgacaggat cgcgtagtcc ccgcagcccg ggcaccagcg
      721 aacctcttgg tcggactcga agtccttctt cgtcagttgc agcacttcgt ctgccatcgt
      781 tgtcacaacc tcttgaggat ctcggtttcg agctcggccg caaggaacgg ctggccctgg
      841 atcttggtgt aggactttgc gtccaccagg tattcggcac gcagcatgcg aaccagctgc
      901 ccgttgttga gctcgggcac cagcacgcgc gagaagcgct tgagcacctc gccggtgttc
      961 ttgggcatcg ggttcaggtg ccgcagatgc gcgtgcgcga ccttgtggcc ctgggcgcgg
     1021 acgcggcgaa ccgcggcctg gatcgatccc caggtcgagc cccatccgac gaccagcagc
     1081 tcttcgccgc cctgctcgtc cacgtgcagc ggatcgatgt cctcggcgat cttggcgacc
     1141 ttcgccgcac ggacgcgcgt catgtactcg tggttggccg ggtcgtagga cacgttgccg
     1201 gtgccgtcct gcttctccaa gcccccgatt cggtgctcca ggccgggcgt gcccggcacg
     1261 gcccacggac gcgccaggtt ttcgtcgcgc aggtacggcc agaactcgcc gcccttgttc
     1321 ggctcggtcg cgaacgtgtt ttcgatgacc ggcaggtcct tcacgttcgg gatccgccag
     1381 ggctcggccg agttcgccag gaacgagtcc gacagcagaa acaccggcgt ccggtatttg
     1441 atcgcgatcc gcgccgcttc gatcgccgcg gcgaagcagt ccgagggcga ctgggccgcc
     1501 acgatcggca tcggcgcctc gccgtgccgg ttgtacatcg cggccagcag gtccgactgc
     1561 tcgatcttgg tcggcagacc cgtggacggg ccgccgcgct gcacgtcgca gatgatcatc
     1621 ggcagctcga ggatcacggc cagaccgagc gtctcggact tgaggtcgat gcccggaccg
     1681 gcggttgcgg tcacggccag ctggccggcg aacgccgcgc cgacggccat gcaggccgcc
     1741 gcgatctcgt cctcggcctg cagcgtcgct accccgaagt tcttgtgctt cgacagctcg
     1801 tgcagcacgt ccgaggccgg cgtgatcgga tacgacgcgt aatacagcgg caggctcgca
     1861 cggtcggcgg ccgcgatcag gccccaagcg agcgcggtgt tgccggtgat gttgcgatag
     1921 gtgcccttct tcagcaccgc cggcttgacc tcgtagtggc tggcgaacag ctcggcggtc
     1981 tcgccgaacg cgtggccggc cttgaacgcc gcgagattgg cctccatgat cggcggcgcc
     2041 ttcgcgaagc gcttctggat ccattcggtc gtcgggtcga ccgggcgcgt gtacatccac
     2101 gacagcaggc ccagcgcgaa catgttcttg gcgcgctcgc tgtccttctt ggtgacgccc
     2161 tcgatgcctt tggtggcgtc gacggtcatc tgggtcatct tgaccttgat gagctgatat
     2221 ccgtcgagcg agccgtcctc gagcgggttc ttgtcgtagc cggccttctc gaggttgcgc
     2281 tccgtgaatg catcctcgtt gacgatcagg aagccgccgg tctgcaggtc cttcaggttc
     2341 gtcttgagcg ccgccgggtt catcgcgacc aggacgttgg gctggtcgcc cggggtgagg
     2401 atgtcttcgc tggagaagtg gatctggaag cccgagacgc cggccagcga gccggcggga
     2461 gcgcggattt cggcggggaa atccgggaga gtcgagaggt cgtttccgag gatcgcgctg
     2521 acgttggtga agcggtctcc ggtgagctgc atcccgtcgc ccgagtcgcc ggcgaagcgg
     2581 atcaccacgc gatcgagcga gatggtttcc ttactcattt ctcctctttc ccgttccaac
     2641 gaaccgatga cgcgcaacat tccgcatcgc cggttggttg gagccgacgc cgacgtacct
     2701 gccacccacg agcgacagac gcccccgccc ggcgcccgca gggttcgacc gccgagtcgt
     2761 tcgactgcgg cggccggaca agtcattgta tcgccgcagg tcagagcggg cgaacagcat
     2821 gggcctttga gtcatgtttt tcttctaaag aatgccttcc cgcgaggccg ggacccgaac
     2881 ctatacttgc cgcgtgaggg agatctacct cgatcacgcg tccacgacgc cgccgcatcc
     2941 ggccgcgctc gaggccatga gcaaggccgc caccgagctc tggggggatc cgtcgcgtct
     3001 gtaccgggcg gctcgtgtcg ctcgcatcgc cttggacgaa gcccgggcag cggtcgcttc
     3061 ggtgatcggc ggccggcccg aggagatcgt gttcacctcg ggcggaaccg agtcgtgcaa
     3121 cctggggatc gtggccggcg cccgcgcggc cgccgcggcc cgcaagccca agcgcatcgt
     3181 ggtcccggcc gtcgagcaca ccgccgtgct cgagtccgca caggccctgg acggcttcga
     3241 gatcgtccgg gtcggcgtgg acgagcacgg ccgggtggat ctggacacgc tgcgcgacga
     3301 atgcgcgcgc ggcgccggct tggtcagcat ccaggccgcc aaccatgagg tcggcacaat
     3361 ccagccgctc gccgacgcgg ccctgctggc gaagcaagcc ggagcgctca ttcacaccga
     3421 cgcctgcatg gctgtcggcc agatcccggt agacgtcaag gcgctcggcg tggacctgct
     3481 ctccggatcg ggacacaagg cctacgggcc gcgcggaacg ggctttctgt gggcccgaac
     3541 cggcgtgcgc gtccggccgg tcatggtcgg cgacgaccgc gagcgccgcc gccgcgccgg
     3601 cctcgagaac ctgcctgcca tcgccggcat ggccgccgcg ctcaccgccc gcgccggcga
     3661 gatcgacgcc gagatgccgc gccttcgcgc gctcaccgac cggatccgca cccagcttcc
     3721 ggccctgatc gaggacgtcc agatccacgg ccatccgacc gagcggcttc ccggcctggt
     3781 cgcgttcagc ttgctgtacc tggaaggtga agccctgttg ctgatgctcg acggaaaagg
     3841 gatcgcggtg cacagcggct cgtcctgcgt gtccagcgcg caggagccct cgcacgtgct
     3901 cgccgcgatg ggcgcgctca cgcacggcag cgtgcgcgtc tcgctcggcc gcggctccac
     3961 cgacgaagac gtgaacgcgt tcttgaaaga gcttccgccg atcgtggcga acctccgcga
     4021 gatcgcgcgg accggctcgg catgagcgag cagccggccg cgacgctgga cgtgctcggc
     4081 cttccctgcc cgatgccggt cgttcgcttg tccaagacga tcggaaccgt acccgtcggg
     4141 gccatcgtgg aggtcctgtc cgacgacgaa gccgccaaga tcgatatccc cgtctggtgc
     4201 cgcatgaaga agcacgagta cctcggcgtg aaggcaaccg aacgcggacc ggcccatctg
     4261 gtccgccgca tgctctagga gggcacatgg aagccgtata cgggatctgg gtcctggcag
     4321 gattcgtctt cttcttctgg ttcgtcgcga ccatgcacgg catcgccaag gaccttcacg
     4381 cgatccgcga agcggtcgcg ccagagccat cgacaccccg gtccaaggcg taacatccgc
     4441 tttcgcccgg ttcggggccg cttcggtgca accgatcggc cgccgatcag ttatcgaagt
     4501 catgagcgcg atcccgcgcg cggcgacgcg cgccctccga agcccggccg ggcataccga
     4561 gccgatcggc ggccgcaacc ttgccgtcga gtcgctccgg ggcatcgcgg ccctcgccgt
     4621 cgtgggctat cacttggcgg ccgtcactga gtggccggcc tcgttcccgc cgcgcttgct
     4681 gctatccaac ggcgacgcgg gcgtcacgct gttcttcgtc ctgtcgggct tcctgctgtg
     4741 gcggccgttc gcgaaggctg cgatcgaggc cgggccgatg ccgggcctcc gccagttctt
     4801 caccaaccgt gcgcttcgga tcgtccccgc gtactgggtc gcgctcgcgg tcacgatcgt
     4861 cgcgctgggc gcgaccgcgc gcggcggcgc gtggcgatac ctgatcttcg cgcagaacta
     4921 ttcgcgcgag tcgttcttcg gcgtgatctc gccggcctgg tcgctggcgg tcgaggcggt
     4981 cttctatctg gtgctcccgg tcgcgggctt cgccgcggtg cggcttgcgc gcagcgcgcc
     5041 gagcccgatc cgggccgtct acggcgtcgt cggagcatgg ttcgccgccg gcctcgccta
     5101 taagctctac gcactcggcg tgcaggacgt cgatccgacc gatcccctgt tcttgttcaa
     5161 cttccccgcg aaggcgcacc tgttcgccgg cgggatgctg ctggcgacct ggcacgcgcg
     5221 cgaccggatg cgctcgccgc tcggctcgtc cggccggtcg gccttgatcg gatcagcgat
     5281 cgtcgtcgcc gcggtcggga ttcgctatcg cagcgcggcc accgccgcgt tcttcgacac
     5341 gctcaccgcg gccggattca cgcttatcgt cgccgccgtc gcctacgggc agccgcgtct
     5401 ttcgcgcctc tcccggagcc ttcactggcg gccgctgatc gcggccgggg tcgtgtcgta
     5461 ctcgatctat ctgtggcacc acccgctgat cgagcggctc cacgaggcgg gcgtgctgcg
     5521 ctcggagttc gcctggctgc cgctgaacat cgtcgtcgtg ctcgcaatca ccatcgcggt
     5581 cgcggccgcg tcctacctgc tgatcgaacg gccgttcctc aagctccgcc gccgctggtc
     5641 ctagcttctc cgagaaggac ctggccgtcg cggggtcgaa gtacccgcgt atgaaaccga
     5701 catttcgacg tgtcgccgcc tggctcgccg cgtgcgccgc cgcgctgtcc acgctctcgg
     5761 ccggagcgac gatcccgatc gacgacccgt gcgcgcggta ccgggcgctc ggcgggaacc
     5821 tgacggccga ggtctccgac tcggctccgg gcagcgggcc gctgcgcgtg ttcgcgatgc
     5881 agcacaagca gctcgtcgac tacgtggaga cctacgagac gtacgcggcc aagatggact
     5941 gcatgatgcg cgactacgtc gcgccgcacc tcgatcggtc caagaccaac ctgatcgtgt
     6001 tcaacgaaga catcggcttg atcacgctcg gcaccggatc gcgcggcgcg caggcgcgcg
     6061 cgctcgccgc ctcgccgatg ccgggatcca cgcccgagac gctcgacgcc accggagcgc
     6121 cgctcggcgg tgcggcggcg ctcggctcgg tcgccgcgac ctatcagcgc cagatgctcg
     6181 acgtggagcg ccgcttcccc ggaacggacc cgcgcaaggc gatcctgctg gccgcgaccg
     6241 acacgttcgt gcgcggtttc tcggcgacgt tctcacggct ggcgcggacc tacggcgtgt
     6301 ggatggtctc gtcgaacaat cagggcgagt tcgactactc aaccgatccg gtggacgtcg
     6361 cgacctgggc ggatcccgat ctcgccgccg actacgcggc cgggaggatc aaaggcgtgt
     6421 tcgttccgcg cagcgagaag gtgtggaaca acgccttttt gtgggcgccc tacgatcttc
     6481 ggccgcccaa gccgggcgcg tccgccgacc cggcatcgcc cgactacgat ccggacacga
     6541 acctgctctt caccaaccgc aagacgccgc tgaccgagat cgagaaggcc ttcttcgcgc
     6601 tcgacgaagg agacatgagc gtctccaaca ccgggccggt cgagattccg ggccttgccg
     6661 gcttcaagct cggcttcgcg atctcgctgc cggcgttccg cttcggcaac gatttcggag
     6721 agccgttctt cggcgatccg tgcgcttcgg cgaactcgtg gatgcgatgc ctgcacgagc
     6781 gcggcgtgaa cacgatcatc cagccggaag ccaaccccgg cccgtgggcc cgctacggcg
     6841 ccgacggcgg caacttccag tcgctgacgt ggggcgcctc gacgcttcgc atggtgaccg
     6901 atccgacggt gccgaacttc cgctacgtcg tctgcccgca catgaccggc aacctgatcg
     6961 acctgccgtt cgacggacag agcgcgatcc tcgagcgtgg caggaccgga acggcccgcg
     7021 cgtacgtcgg agcgaaggac ttcatccccg aaaccgactc ggcatggacg atgccctatg
     7081 cgggcctcaa gcccgagttc ctcgccctcg cagcctgggt catcgacgac gaccactcgc
     7141 tctccccgat cgagaaccgc cgccggctcc ataacagagc gagcgagctg atggccggat
     7201 cacgctccga gcattcgaac gcatacctgg aaaccgcgat cttcgcggat ctcgccactc
     7261 cgtagcgggt agaggcgcgc gctatcatcc catctgaggg ccatgatgca gccaggcgaa
     7321 accctcgagc gcgaagccgc gcagctctac tacgaccacg cctacgccgc cgccgcccaa
     7381 cgctatgagc gtgccttcgt cgcctatcgc ggcgaaaaca accacctcgc cgccggcctc
     7441 gccgcgcgcg cgatcggctg gatcaccggc aacgtgctcg gcgattgggc ggtgcagaac
     7501 ggctggttcg cacgctcgct cgcggagttc gagaaagccg gcgatcccgt cgctcgcggc
     7561 tgggcgctca ccgttgctgc cgacctggag atggactatc ccaagcgtgc gccgctctac
     7621 gcagacgcgg tcgcggtcgc acgggaacac ggaaatgtcg atctggagta cgaggcccac
     7681 gcctacctcg ggctgctcta cgtcatggag ggccgcttcg aagaaggact cctgtacctc
     7741 gacgaaggtc ttgccgccgt atgctcgggc gagatcaccg acatcgtcgt cttcgacggc
     7801 atcttctgcg ggttcttctg ggcctgcgag atgctcaacg acgtcgcgcg tgccgaccag
     7861 tggatgcgcg cggccgcgga catcatgcac gggcggcaga tcgtcgcggc attctgccgc
     7921 gcgcactacg gcgccatctt aaccgctgcg ggccgctgga aagaagccga gcacgagttg
     7981 ctccaagcct ctcgccactt ccaaaccagc agcaccccga gacgatcggc ggcgatgatc
     8041 cggctcgccg atctccgcgt gcgccagggc aggctcgaag aagccgcgca actcctgcgc
     8101 ggcgccgagc aggatcccga cgccgcgcgt ccgctcgccg ctctgtactt cgcgcgcggc
     8161 gagacggccc gcgcgcgaga cctcctcgag cgggcgaccc agggcgaagg cgtaccggcg
     8221 ttgattggtc ctctgctcgc gctgctggtc gaggtctcgc tcgctgaagg gaacgtggag
     8281 caagccacgc acgcggcgga gcggttggaa agcctctcgt cagtgcacca cgttccttac
     8341 ctccgcgcgg ctgcggcgct cgccgagggt cgtgtgtgca tcgcgaccga caccggcgac
     8401 gcgcgtgcgc gcttgcacga ggcgctcgaa gggttcggac gagcgcagct tcccatggag
     8461 ctcgcgatgg cgcacctgca catggcgcgc gcacaagcag aacggtcgcc cgaggccgcg
     8521 atcgccgaag cgaaggcggc gctcgaggat ttcgagcgtc tggaggcggc gcgccacgcc
     8581 gacgaggcgg ccgccgtgct gcgcgggctc ggcgcgcccg cccgcaccgg gccgaagggt
     8641 ggtggcaccc tcacgcgacg cgagacggag gtgctgcggc tgatcggctc cgggctgtcg
     8701 aacggcgaga tcggcgatcg gctctacatc actcgaaaga cggtggagca tcatgtcggc
     8761 tcgctgttga tgaagctcgg tctgcgcaac cgcgccgagg ccgccgccta catcacccga
     8821 gaagaatcag gccactaaat aggggaactc cccgatgctc gggccggccc ggggcgagca
     8881 tgctgtgcgg atccgcacgc gaaaggggag acaatgaaga cccatgacgt gatcgtggtc
     8941 ggtgcgcgct gcgcgggttc gccgaccgcg atgctgctgg ctcgcaaagg gtacgacgtt
     9001 ctgttggtcg accgtgcgac gttccccagc gacacactat cgacgcatct gctgcacgcg
     9061 ccgggcgtcg atgcgcttcg gcgatggggc ctgctggacc ggctgatcgc cacgggctgc
     9121 ccgccgatcg agtcctacaa gttcgacttc gggccgctcg tcatcgaagg ggcaccgggg
     9181 acggcctacg cgccgcgccg cacggtgctc gacgagctgt tggtgaacgc ggctgcggag
     9241 gccggcgccg aggtgcggga gggcttctcg gtcgaggaag ttttgatcga ggacggccgt
     9301 gccgtcggaa tccgcgggca cggcaaagac tgggcgagcg taacccagcg cgcgcgcatc
     9361 gtcgtcggtg ccgatgggcg ttactcgagg gtcgcgaagg aggtaaaacc cgagcggtac
     9421 aacgagattg gcccgatcct cagcggctac tacgcctact ggagcggcct tccggtcgac
     9481 gggttcgaga cctacgtgcg accggatcgc agctgggcgg tgtggccgac ccacgacggt
     9541 ttgacgctcg tgatcgccgg ctggccatat gcggagttcg aggcgaacaa gagtgatatc
     9601 gaggggaact acctcaagat gttcgagctc gcgccggagt tcgacgcgcg catccggggc
     9661 gcgaagcggg agtctcgctt cgcgggcaca gcggtcgaga actacttctg caagccgtac
     9721 ggtcccggat gggctctggt cggcgacgcg ggatacaaca aggacttcgt caccgcgcag
     9781 ggaatgtccg acgcgttccg cgacgcggag ctgtgcgtga ccgcgctcga cgaatcgttc
     9841 ggtggcgcac gctcgttcga cgatgcgatg gagacctacc acaccacgcg cgacgagcga
     9901 tcgatcggga tcttcgagtt cacgacgatg ctcgcgacgc tggagccgcc gccgccggag
     9961 acgcagcagt tgctcggcgc gatggtcggg aatcaggacg cgatagacgg cttcgcgcgc
    10021 gtggttgccg gggttacgcc gccgtcggag ttcttctccg aggccaacgt ggggcggata
    10081 ttcgagcagg cgtctgcgat cgcgacggcc tagggtcagc cggcggacac gagtggtcac
    10141 gcgtcgccga actcgcggac gcgatgcctg cacgcacacg gcgggagcgc aatcctggag
    10201 cacggccgca ccggccaggc acgcgcgtac gtcggggcga aggacctcat tcgcgagacc
    10261 gactcggcat acctggcaac ggccatcttc gccgacctgc gcacgcctta ggagcggcgt
    10321 ttcttcccgg gccgcacttc ggtgaagccg gcggccgtga agtccccgtc gaacgcgagc
    10381 gcctcgcgaa tcttcatgcg ccgcatcgtc gcgaagctgg tcgcgtccac gaacgagtat
    10441 tcgcgttcgt cgtggcggcg cagccacgcc cacgcatcac gctcggtctt ttcatccacg
    10501 tgcacgatgc tcagcgcagg cgatcgctgg gcgcgatcca ggaaaccgac ggcgaattga
    10561 tgaccggcac gacggttcag atacgtccag gtctcgccca gaatcagcgt cgtcgtcagc
    10621 agcgagccgg cgccccacaa agcgcgggcg tcccgatggt tgcggtcccg cgagacctgc
    10681 agcgcgaccc aatacgacgt atcgacgaac ttcatcgtcc gtagacgacg tcgtccaccg
    10741 actcggcctc gacgtcgagc cagcccacga cttcgtccaa cggatccgcc ttcgcaggac
    10801 gaagcttctc tgcaacgaat cgcctgatca gcgccgcctt cgacgtcccc tctcgcgacg
    10861 cctccaccgc cagccgctca tcgagctctt cctcgatata gatctggagc cgcttcatgc
    10921 attgagagta tacagcatac aggacaccat atacattaca cccctcgttg accatgcgcg
    10981 ccacatgtga catgctcggc acatgtcgaa aatgatccag gtgcggaacg tcccggaccg
    11041 gttgcatcgt gaactcacga agagagccaa ggcccgcggc cagaccctga ccgattacat
    11101 ccaagagctg ctggagcggg atgtggccag gccgccggcc gtcgaggtgt tcgaccggat
    11161 cgaggcgagg aagcccgtga agctcggccg ccgggctgcc gatctcatcc gcgaggagcg
    11221 ggacggacgg gatgcctcct gagggtcatt gtttccgacg cctccgcgct gatcgaatac
    11281 ctcctcggaa cggatgcggc cggccccgtg agggacatcc tccggctccc ggacgtcgat
    11341 ctgcacgttc ccgccctctg cgacgtcgaa gtggtatcgg ggctcagacg ggcaacgcgg
    11401 cggaggctgc tttccgatcg ccgcgccgcc gaagcgctgg acgattacgt cgatcttccg
    11461 ctgagccgac acggccaccg gtccctgctc gcccgcgtat tcgagctgcg agcaaacttc
    11521 tccgcctacg acgcgacata cgtggcgctt gccgagcgga taggcgccga gctgctcacc
    11581 gccgacgagc gactcggccg ggccgtccgg gcgcaatccg ccgtcgcaac gattccctga
    11641 tccaggttag gccacgggga tgacactcga gccgcgtccg acgccggctc cctaggtcgc
    11701 cgcggtcgcc ccttgcgcgg cccggcgaca cgaagccgcg atggtttacc gcggcgtgca
    11761 gcggcacgaa ccggctccaa cgcggcatac gtttcgccgg gcgcaacacg ttttctcggc
    11821 tctcgtttct tgcgtgaaag cagcaagtga attcgtccct cccatcgatg attgctgcgg
    11881 tttgcgcccc ggagagattg cgaaaccttt ccggaactcg cacaaccttc ggcttcacac
    11941 agccgatagg gatgagacgc gtcgtccgaa cggactaccc actggagacc aatgagcccg
    12001 atcgcccgcg ccctcatcgt ggctggaatc gccgtttcac tggtcgcatc ggccatggtc
    12061 gtcgccgatc gaaacgcacc cgcgcgccgc gttgccgccg ccgacgacga cttgcttgct
    12121 tcgccgcccg aagacgccgc cgtcgccgcg acacgcttcg ccgccgcgat tcacggcgtt
    12181 cccgtccgcg acggcgaccc cgacgattcg tcgcgctact gctcgaccga ccggctgcgc
    12241 gtcctgtgga ccgcgccggg tgagggctac acgcacggcg gttacatcga gccgctcggc
    12301 ccagaacctg gaccggacac cgttgcggtc aacggcgtgg tggtctgcga aggttcggcc
    12361 tacgcgtaca tgggcttcga agcgctgcgc gacggcgacg gctggcagat cattcccgtg
    12421 ccggcgatgc tcgatgacga ctccgggaac gacgaggaga cccccgggaa cacggtcgat
    12481 cccggcctga ccgcgaaggc gtcaccgccc gccgcgaccg cggcggcgtc tgggaaggtc
    12541 ccgacgcgct cgaccgcgac gttgaccgcg gcgatcgaga cgtatgccgg ctacgacgcc
    12601 caagacacgt gcagcccacc cgccaagccg ggcgtgctcg cgttccgcaa gctcgtgctg
    12661 ggcacgtatc cggggacgcg cagcttgggc atctcgcgcg gctgcggcgt cggcggacgc
    12721 agcgagcaca aggaaggacg cgcctgggac tggggcgtca acgtcaaccg tcccgccgag
    12781 cgcgccgccg cagacgcgct gatcggctgg ctgctcgcaa cggacgagtt cggcaaccgc
    12841 cacgcgatgg cccggcgact cgggatcatg tacatcgtat ggaaccgcac gatctggggc
    12901 tcgtatcgct ccgatcaagg gtggcgcgcc tacaacgggg cgagcccgca caccgaccac
    12961 gtgcacttca gcttcgcctg gcccggcgca aacggcgcaa cgagcttctg gaccggcgcg
    13021 gtcgccacga tgcccggcgg caacggcgga ggcgggtcgt tccgcgacgc cggaggcggc
    13081 ggttcggtgc gcggcgtccc acgcgacggc gagcgctggt ggaggaaccg caccgacgaa
    13141 ggcacgcgca ccgacatcca gcgcaagcgc accacgcgaa ccgagccgat ccgctggcaa
    13201 gaccgatccg gccgctggtg ggagtgggac cgcgagacgc gccgctggaa gcgcatcgac
    13261 gatcccagcg cgacgccgac cccgacgccc gagccggaac cgaccccgac gctcgagtcg
    13321 gatccatcgc ccgagcccga atccgaaccc aagtcctcgg gcacgcccgc gccatccccg
    13381 tcgatgtccc cccggccgtc gccgtccgcg accagcggga cggttcgatg ggacggcagc
    13441 cgcaccggcg gcgaccgcac cggcacgacc gaagagcgcc gccgcaccac gcgcgaagag
    13501 cgccgcatcg aacgcgaagc caagcgccgc cagcaacgcg aagtccggcg caacgagcgc
    13561 aaacgaaagc gtctcgagca gaaagaacgg cagcggaagc gtcgcgagca gcgcatgcgc
    13621 acccgcacca cgaacggcta atggaaatgg ggctgccacc agttccacta tcccgcccag
    13681 cagctccgcc cagaacgcgc tcttggccgc ccttcaagcg gcacgccggc cgatctatca
    13741 aggcgggcgg gggaagcccc cgaggcgcgc tcgctctttc cgagcagagg caaccgacgt
    13801 agcatcaggc cttcgaacgc tcgatacggg gggcacgatg ccggtcatta ctgggattca
    13861 tcatctgtcg ctgacagtcc gggacggagc ggccagcgaa gagttctatt ctcgcgtcct
    13921 ggggttcgtc ccggtcctgc accttcccga tgcggagggt cgcggcgcca agcacgtcat
    13981 ggcccacccg gcatccggaa ccgtcgtggg attcaccgtt catcaagcca acgacggctc
    14041 cgcgttcacc gagttccgta ccgggctcga tcacttcgcg ctgggcatct cgtctcatgc
    14101 cgaactcgac gagtggatcg ccagactgga cgagctcggc gtcgaacacg gggacgtggt
    14161 caagactccc gtcggcgact acgtcagcct gcgcgatccc gacaacgtcc agatcgaatt
    14221 gtgggtcgat cctaagtagt gctcatggca accggcgtcg tcgttctgga aacacatctg
    14281 cactatctgc gggctttgct gacccgagga agcggatcgg tccaggatcg agacggagtc
    14341 ttggtgtatc aaagtcccgc tcccttgccg ttcctcgtca acggtgtcgc acggaccgat
    14401 ccctctgttg ctcccgccgc ggtgctcggt gcggcggccg ccgccttcgg cgatcgcggc
    14461 ttcgaagttc tgtgcctgga cggccgcgac gacgatctgc gcgccgccgc cgaagcagcc
    14521 ggatttcaaa ccggatcgcc ggatccgctg caagccaccg ctcgcgtgcc cgacccggct
    14581 ccgatcgacc attccgcgtt cgacatccgc ctggccgaat atgccgcgga cgtcgatgac
    14641 atcgtgacga tcgcagtcga tgcgcacacc gtgtataagt ttcccgacgc cttcttccgt
    14701 gagatctttc gaatcccgga atcgattttg tcacccgaca tccgcgccgt gatcgtgtcg
    14761 cagcagggca caccggttgc gacagcgcaa gcttttctga ccggcagcat cgtctacatc
    14821 ggatgggtcg ccgtcgttgt cgccgccgct cgccgcggac tcggctggct cgcaacccac
    14881 gaggccgtcg ctgccggctt cgcgagcggc gccaaagaag ccgtgcttct ggcctcgccg
    14941 atgggcgcgc cgctgtatcg aacgatgggc ttcaccgatg tcggcacgct gagaaacgcg
    15001 caggcaccgc cggcacgagg ctgactccgg attgatccca gctcgggtca cgcgcgtcga
    15061 tgcccacatg gaccgcctgg aatatgccgc tggttgaccg cacacgaagc ggcaaggaca
    15121 tgccgagcgt cacgaccgcg cccccggccg tgaccagcca catcccgggc atgacgatcc
    15181 ccaacagcag gaccagccga gcggcgtttc gcgcgaagat ccgacggcct caatcgcaat
    15241 gtgatgtcga tgaccgcgat cgcaagggtg atacgggaaa cttgctcgag caggacgtgc
    15301 cgagcacgat gatcgaaagg cccaggatcg cgagcacggc gtcggcagtt acgaaaatcc
    15361 tggtttcccg ccatcgagga ccacgaaccc gagcccggcg gtaatcggcc gggggccgga
    15421 accgcgacga gcagtgaggg cgaggacggc ctgttagtct cgctttcgtg gcagtgcttc
    15481 tgctcgtcta caacctggcc ctcggcctgg ttgaggtcgc gctcggcgtt cgggcggcgc
    15541 gctcgcgccg atcaacggac gcgctgattc tcgtcggcgc tagcgccgtg ggcgcgttcc
    15601 tgttcgcgct cgtgatcgcc ggcggacccg gctttcgaat gctgcggctg ctgtcgtggg
    15661 gcctgttcgg acacgtggtg gtgtctctcg tcgcggccgc ggtgttgacg cgtcgaacgc
    15721 ggtggatggc ggcgatcttt gccgcgtgcg ccattgccgg cggctcgatt ggcgtctatg
    15781 cctttcggat cgagccgttc atgctggagg tgtcgcggta tcggatcgaa accgagaagc
    15841 tcgaccggcc gctgcgcgtc gcggtactcg cggatttcca gagcgacgag atcggagact
    15901 acgaacgccg cgtgatccgg accgtggccg cgcaggacgc ggatctcatc ctgatgcccg
    15961 gcgactac
//
LOCUS       DATLFO010000059         4035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_80388, whole genome shotgun sequence.
ACCESSION   DATLFO010000059 DATLFO010000000
VERSION     DATLFO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80388"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VM841_02060"
     CDS             complement(<1..665)
                     /locus_tag="VM841_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HVL88998.1"
                     /translation="MRPHVELIHEDDYIWHPAELPLGEGKARQRNLSLDEEDGSASTS
                     VEFLSAFFRPAGRHVADTEWYVLDGEIAIGDRVLGKGGYFHAPKGVAIPEVAAKEGTR
                     ILLFREYGDWGFEETDQTAGDAQEKDLIVLDTEAMAWEAVDKPGPKPGLMIKMLHRNP
                     ITGFYSRLIWAKPGWDDHRLAHHPCYEEAYTVFGSMVYNFGDLTPGTYFFRPAWVKHG
                     HFIS"
     gene            809..3634
                     /locus_tag="VM841_02065"
     CDS             809..3634
                     /locus_tag="VM841_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVL88999.1"
                     /translation="MHEWPHRPSGPADRPSVVVGASRVSHLVRLGELGSTLTGPDQLD
                     DVLDQALVAVIEIAGARGATVSVEPAPGQKARTCLAGECAAFARPMDLVGLGGEPVGT
                     LTLYFSSIDAQPSEPHLVEAYARRLAEILERTRLSGDKQDLIARERRRAEQLQGLAGA
                     AIQITTAETVNDVLRRVTESAQAIIGTHQAVSSRLVKGWDNAETFISLSDKYGTYRDY
                     DETPQGLGVLNAVTRENEPLRLTGAQLAAHPEFRGLADAPGHPPLPNYLAAPFIGHDE
                     TNLGLIQLSDKVDGTEFTKEDEAILVQLGQMASVAIETIEAIERERAAREEAEMTSLA
                     LAASLEQRSHYERVLAAQADLFEAIASGTALEDLLSMIIETIESLTTDAISSILLLED
                     GRLRHGPSARLPDFYQEAIDGLEIGPDVGSCGAAAYRREPVVVTDVMTDPKWEAFRDL
                     AKRSGFAACWSTPIFSGAGEVLGTLAVYYRDRRGPTPTEESVIKVFTRTAAVAIERSR
                     DLEHLVREQRIVQTLNDVGRAIGSQLNADEIVQQATDAATELTKAQFGAFFYNVIRAD
                     GEAYMLYTLSGAPREAFEKFPMPRNTAIFEPTFHGRGTVLLHDVTADARYGHNAPYRG
                     MPEGHLPVRSYLAVPVKSSGGEVVGGLFFGHAEAGVFDEDAARVAEGIAAHAAIAIEN
                     ARLYESARREGEAARRAYEQRDHVARVLQASLLPPTLPDIPGARVSATFRPGTSDVGG
                     DFYDVFPVRGRDWGLVIGDVCGKGADAAALTALARHSLRTAAMLQRQPSRVLGILNEA
                     LLRGETDGRFATAAFVRLTPSSRRAKLVLASGGHPPIALLRSWGDVMMIEGSGSLLGV
                     FADAGIGDQIVELHEGDSLILYTDGVTEARRGDELFGERRLAEVLRACRGLSAEDIVE
                     RIEAAVASFQDDGKRDDLAVVALTIQG"
     gene            complement(3646..>4035)
                     /locus_tag="VM841_02070"
     CDS             complement(3646..>4035)
                     /locus_tag="VM841_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89000.1"
                     /translation="GHDHAAPDRREAGRGETLVLDAARLASVGSGVVVAGAAIAAFGV
                     GLGAVVRHQVPAVVGGILWFLLAEELLSVAAGEASRFLPAALAQGASYAALGAGLSPP
                     AAALGFTVYTAGLLALGSVLMRRRDLS"
BASE COUNT          652 a         1449 c         1350 g          584 t
ORIGIN      
        1 gagatgaagt ggccgtgctt gacccacgcg ggacggaaga agtacgtgcc gggcgtgagg
       61 tcgccgaagt tgtagaccat cgacccgaag accgtgtagg cctcctcgta gcacggatgg
      121 tgggcgaggc ggtgatcgtc ccagccgggc ttggcccaga tcagccgcga gtaaaagccc
      181 gtgatcggat tgcggtgcag catctttatc atcaagccgg gcttcgggcc gggcttgtcc
      241 accgcttccc acgccatcgc ctcggtgtcg agcacgatca ggtccttctc ttgcgcgtcg
      301 ccggcggtct gatcggtctc ctcgaagccc cagtcgccgt actcgcggaa cagaaggatg
      361 cgcgtgcctt ccttcgccgc cacttcgggg atcgccacgc ccttcggcgc gtggaagtag
      421 ccgcccttgc cgagcacccg gtcgccgatc gcgatctcgc cgtcgagcac gtaccactcg
      481 gtatcggcca cgtgacggcc cgcgggacga aagaaggccg acaggaactc gacgctggtg
      541 gaggccgaac cgtcctcctc gtcgagcgac aggttgcgct ggcgcgcctt tccctccccg
      601 agcggaagct cggccggatg ccagatgtag tcgtcctcgt ggatgagctc gacgtgcggg
      661 cgcatggcga aaaacccccg tttcgttcag cggtacggtg tttcgaacag ggtacccgcg
      721 gcaggaagac agggtcaacg gcgaggtttg gacccctcat cgatcgggta taccagttcg
      781 aaccgtcatc gtgggggtct cattgtcgtt gcatgagtgg ccgcaccgtc cgagcggccc
      841 ggccgaccgt ccgtcggtcg tggtgggcgc gtcccgcgta tcccatctgg tccggctggg
      901 cgagctcggt tccacgctga ccggccctga ccagctcgac gatgtgctgg accaagcgct
      961 cgtagccgtc atcgagatcg ccggggccag gggcgcgacc gtgagcgtcg agccggctcc
     1021 cggacagaaa gcccgcacct gcctcgccgg cgaatgcgcc gcgttcgccc gcccgatgga
     1081 tctcgtcggc ctcggcggcg agcccgtcgg aaccttgacc ctgtacttct cgagcatcga
     1141 cgcgcaaccg agcgagcctc acctcgtcga agcctacgcg cgacgtcttg ccgagatcct
     1201 cgagcgcacc cggctcagcg gcgacaagca agacctgatc gcgcgcgagc ggcgacgggc
     1261 cgagcagctc caaggcctcg cgggcgcggc gatccagatc accaccgcgg agacggtcaa
     1321 cgacgtcctc cgccgggtga ccgagtccgc ccaggccatc atcggcacac atcaggccgt
     1381 gtcgagccgg ctcgtgaagg gatgggacaa cgccgagacc ttcatctcgc tgagcgacaa
     1441 gtacggcacc tatcgcgact acgacgagac tccgcagggg ctcggggtct tgaatgcggt
     1501 cacccgcgag aacgagcctt tgcgcctgac cggcgcgcag ctcgccgcgc accccgagtt
     1561 ccgcggactc gccgatgcgc cgggccatcc gccgctgccg aactatctcg cggccccgtt
     1621 catcggccac gacgagacca accttggcct catccagctc tcggacaaag tggacggcac
     1681 cgagttcacg aaagaggacg aggccatctt ggttcagctc ggccagatgg cctcggtcgc
     1741 gatcgaaacg atcgaggcga tcgagcgtga acgcgccgcg cgtgaggaag ccgagatgac
     1801 cagcctggcg ctggccgcca gcctggagca gcgaagccac tacgaacggg tgctcgccgc
     1861 ccaggccgat ctgttcgagg ccatcgcgag cggaactgcg ctcgaggatc tcctttcgat
     1921 gatcatcgag accatcgagt cgctgaccac tgacgcgatc agctcgatcc tgctgctcga
     1981 ggacggccgc ctccgtcacg gcccctccgc gagacttccc gacttctacc aggaggccat
     2041 cgacggactc gagatcggac ccgacgtcgg ctcgtgcggc gctgccgcct accggcggga
     2101 gccggtcgtc gtcaccgacg tgatgaccga tccgaaatgg gaggcgttcc gcgatctcgc
     2161 gaagcgttcg gggttcgcgg cgtgctggtc cacgccgatc ttcagcggag ccggcgaggt
     2221 gctcggaacg ctcgcggtct actatcgtga ccggcgcggc cccaccccga cggaagagtc
     2281 ggtgatcaaa gtcttcacgc gcaccgcggc cgtggcgatc gagcgctcgc gcgacctgga
     2341 gcacctggtg cgcgagcagc ggatcgtgca gacgctcaac gacgtcggcc gcgccatcgg
     2401 ctcgcaactg aacgccgacg agatcgtgca gcaggcaacc gacgcagcga ccgagctcac
     2461 caaggcgcag ttcggcgcgt tcttctacaa cgtgatccgc gccgacggcg aggcctacat
     2521 gctctacacg ctgtcgggcg caccgcgcga ggcgttcgag aagttcccga tgccgcgcaa
     2581 caccgccatc ttcgagccta cgttccacgg acgcggaacc gtgctgctcc acgacgtgac
     2641 cgccgacgcc cgctacggcc acaacgcgcc gtaccgtggc atgccggaag gccaccttcc
     2701 cgtgcgcagc tatctggcgg tgccggtgaa gtcctccggc ggcgaggtcg tcggcggact
     2761 gttcttcggc cacgccgaag cgggcgtgtt cgacgaggac gccgcgcgcg tggccgaagg
     2821 catcgccgcg cacgccgcca tcgcgatcga gaacgcgcga ctctacgaat cggcccggcg
     2881 cgaaggcgaa gccgcacgac gagcctatga gcagcgcgac cacgtcgcgc gcgtgctgca
     2941 ggccagcctg ctgcccccga ccttgccgga catccccggc gcgcgcgtgt cggccacgtt
     3001 ccggcccggc acgtccgacg tcggcgggga cttctacgac gtcttcccgg tccgcggacg
     3061 cgactggggc ctggtgatcg gggatgtgtg cggcaagggc gccgacgcgg ccgcgctcac
     3121 cgctctcgcc cggcactcgc tccgcacggc cgcgatgctc cagcgccagc cctcgcgagt
     3181 gctcggcatc ctcaacgagg cgctgctccg cggcgagacc gacggccgct tcgcgaccgc
     3241 cgcgttcgtg cgcctgaccc cctcctcgcg acgcgcgaag cttgttttgg ccagcggcgg
     3301 gcatcccccg atcgccctgc ttcgctcgtg gggcgacgtg atgatgatcg aaggcagcgg
     3361 ctcgctgctc ggcgtctttg ccgacgccgg aatcggcgat cagatcgtcg agcttcacga
     3421 aggcgactcg ctgatcctgt acaccgacgg cgtcaccgag gcgcgccgcg gcgacgagct
     3481 gttcggcgag cgccgcctgg ccgaggtgct gcgggcgtgc cgcgggctgt cggccgaaga
     3541 catcgtcgag cggatcgaag ccgccgtcgc gagcttccag gacgacggca agcgcgacga
     3601 cctcgccgtc gtcgcgctca ccatccaggg ctgaccacca ggccgttacg acaggtcgcg
     3661 gcgccgcatc agcaccgatc ccagcgcgag cagccccgcc gtgtacaccg tgaaccccag
     3721 cgcggcggcg ggcggggata acccggcgcc caacgccgcg tacgaggcgc cctgcgcgag
     3781 cgccgccggc aggaaccggc tcgcctcgcc ggccgccacc gagagcagct cctcggcgag
     3841 caggaaccaa aggatgccgc ccaccacggc gggaacctga tgccggacga ctgcgccgag
     3901 cccgacgccg aaggcggcga tcgccgcccc ggccacgacc acgccgctcc cgaccgacgc
     3961 gaggcgggcc gcgtcgagca ccagcgtctc gccacggccc gcctcgcgtc ggtcgggagc
     4021 ggcgtggtcg tggcc
//
LOCUS       DATLFO010000060         3206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_1121, whole genome shotgun sequence.
ACCESSION   DATLFO010000060 DATLFO010000000
VERSION     DATLFO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..>3206
                     /locus_tag="VM841_02075"
     CDS             405..>3206
                     /locus_tag="VM841_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HVL89001.1"
                     /translation="MPEKQGLYDPQFERDSCGVGFVVDMKGRTSHAIVDLAISALLNL
                     EHRGATGAETNTGDGAGILIQMPDAFLREVAGVKLPEAGRYAAGMVFLPQNDNARAEA
                     IAEIEALGDEEGLKVLGWRDVPFDDSMIGEQAREMMPVFRQVFIDGNGYDGMDLERRV
                     FILRKRLERTAGVYLPSLSTRTLVYKGMLTCPQLPQFFPDITDQRLETALALVHSRFS
                     TNTFPSWPLAHPYRLIAHNGEINTLQGNRNWMRAREALLSSDLLPGDLERIFPIITPG
                     ASDSASFDEVLELLHMGGRSLPHAVLMMIPEAWENHRSMSRERRAFYQFHSSLMEPWD
                     GPASIAFTDGTVIGAVLDRNGLRPSRYWVTDDDIVVMASEVGVMDVEPAKVVHKGRLQ
                     PGKIFLVDTTKGRIVGDDEIKDELAREHPYDDWLHAGVIRLRELPARERRKPTHSSVL
                     RRQQTFGYTEEELRVLIAPMAKAGVEPVGSMGNDAPIAALSERPRLLFDYFRQLFAQV
                     TNPPLDAIREELVTAMSSVVGPEYNLLEPAPASCRQIELPYPVIDNDELAKLIHVDED
                     GNLPGFRAQIFKGLYPVGSGGSALQARLDEICAEVDEAIASGVRLIVLSDRHSDAGAA
                     PIPSLLLTSAVHHHLIREKTRTKVGLVVEAGDAREVHHIACLLGYGAGAVNPYLAFET
                     VEDMIARGELTGVTPDKAVYNIIKGLGKGVLKTMSKMGISTVASYTGAQVFEAIGLSQ
                     TLVDRYFTGTVSRLGGIELDGIARECAARHRIAYPDRPEARAHRGLELGGHYAWRREG
                     ETHLFNPEVVALLRQAVRDESFDTFKRYSAAVDDQSRNLATLRGLFKIRIGEREPVPI
                     DEVEPVEEIVRRFATGAMSYGSISQEAHETLAIAMNRIGGRSNSGEGGEDPERDHLDP
                     NGDSRRSRIRQVASARFGVT"
BASE COUNT          534 a         1121 c         1054 g          497 t
ORIGIN      
        1 aactggaacg gcttgccggc aggtgatcct ggcggtactc cttcgcggga agcacctcaa
       61 cgagctgcgc ccccctgccg gcaagccgtt ccagttcgtg ccgatcgatc tctcgcatgc
      121 gactcccttt cgtcgtgtcc atccgaccac ggccgacgca cgcctctacg agcatcctcg
      181 cgcgcaagcg gcagaaccgc cagtgccgtt cggaccgggg attccaggtc aggttgacca
      241 cgggccaccc gccgcggccg gacccgagag catcggccgg atttccggct tggcccggcg
      301 tgttatccgg gtgtagtctg actgccccca gggcagcgtc gcccggggcg gccagccatc
      361 catctccatc cgtgtccgtc taggggtgac gcatgttcaa cgccatgccc gaaaagcagg
      421 gactctacga tccgcagttc gaacgcgact cctgcggcgt cggcttcgtg gtggacatga
      481 agggccgcac aagccacgcg atcgtggatc tggcgatctc cgcgctgctc aacctggagc
      541 atcgcggcgc gaccggtgcc gagaccaaca ccggtgacgg cgcgggcatc ttgatccaga
      601 tgcccgatgc attcctgcgc gaggtagccg gcgtgaagct gcccgaggca gggcggtacg
      661 cggccggaat ggtgttcctg ccccagaacg acaacgcccg cgccgaggcg atcgcggaaa
      721 tcgaggcgct cggcgacgag gaaggcctca aggtcctcgg gtggcgcgac gtgccgttcg
      781 acgactccat gatcggcgag caggcgcggg agatgatgcc ggtcttccgt caggtcttca
      841 tcgacggcaa cggctatgac ggcatggacc tggaacgtcg cgtgttcatt ctgcgcaagc
      901 gactggaacg caccgccggc gtatacctgc cgagcctgtc gacgcgcaca ctcgtctaca
      961 agggcatgct cacctgcccg cagcttccgc agttctttcc cgacatcacc gaccagcggc
     1021 tcgagacggc attggcgctg gtccattcgc gcttctcgac gaacacgttc ccgtcatggc
     1081 cgctcgcgca tccgtaccgg ctgatcgcgc acaacggcga gatcaacacg ctgcagggaa
     1141 accgcaactg gatgcgcgca cgcgaggcgc tgctttcatc cgatctcctc cccggcgacc
     1201 tggagcgcat cttcccgatc atcacccccg gcgcgagcga ctccgcgtcg ttcgacgagg
     1261 tgctcgagct gctgcacatg ggcggccgct ccctgcccca cgccgtgctg atgatgattc
     1321 cggaagcctg ggagaaccac cgctccatgt cgcgcgagcg ccgtgccttc taccagttcc
     1381 actcgtcgct gatggagccc tgggacggtc ccgcctcgat cgcgttcacc gacgggaccg
     1441 tgatcggcgc cgtgctggac cgcaacggcc tccgcccgtc gcgctattgg gtgaccgacg
     1501 acgacatcgt cgtgatggcc tccgaggtcg gcgtcatgga cgtcgagccc gccaaggtcg
     1561 tgcacaaggg ccggctgcag cccggcaaga tctttctggt ggacaccacc aagggccgca
     1621 tcgtcggcga cgacgagatc aaggacgagc tcgcgcggga gcacccctac gacgactggc
     1681 tgcacgccgg cgtcatccgg ctgcgcgagc tgccggcccg cgagcgccgc aagcccacgc
     1741 actcgtcggt gcttcgccgg caacagacct tcggatacac cgaggaagag ctgcgcgtgc
     1801 tgatcgcgcc gatggccaag gcgggcgtcg agccggtcgg atcgatgggc aacgacgccc
     1861 cgatcgccgc gctgtccgag cgcccacgcc tgctgttcga ctacttccgc cagcttttcg
     1921 cgcaggtgac caacccgccg ctggacgcga tccgcgagga gctcgtcacg gcgatgtcgt
     1981 cggtggtcgg gcccgagtac aacctgctgg agcccgcgcc ggcctcgtgc cgtcagatcg
     2041 agctgccgta cccggtgatc gacaacgacg agctcgcgaa gctgatccac gtggacgagg
     2101 acggcaacct gcccggcttc cgcgcgcaga tcttcaaggg tttgtacccg gtgggcagtg
     2161 gcggctcggc gctgcaggcg cgcctggacg agatctgcgc ggaggtcgac gaggcaatcg
     2221 caagcggcgt ccggctgatc gtgctgtcgg accgccacag cgacgccggc gccgcgccga
     2281 tcccctcgct gctgctgacc tcggccgtgc accaccacct gattcgcgag aagacccgca
     2341 ccaaggtggg tctggtcgtc gaggccggcg acgcacgcga ggtgcaccac atcgcgtgcc
     2401 tgctcggcta cggcgcgggc gccgtgaacc cgtacctcgc cttcgagacg gtcgaggaca
     2461 tgatcgcgcg cggcgagctg accggcgtca cgccggacaa ggccgtctac aacatcatca
     2521 agggcctggg caagggcgtt ctcaagacga tgtccaagat gggcatctcc accgtcgcgt
     2581 cctataccgg cgcccaggtg ttcgaagcga tcggcttgtc gcagacgctc gtggaccgct
     2641 acttcaccgg caccgtcagc cgcctcgggg gcatcgagct ggacggcatc gcgcgcgagt
     2701 gcgccgcccg tcaccggatc gcctaccccg accgcccgga agcgcgcgcg catcgcgggc
     2761 tggagctcgg cggccactac gcctggcggc gcgaaggcga gacacatctg ttcaaccccg
     2821 aggtcgtcgc gttgctccgc caagcggtac gcgacgagag cttcgacacg ttcaagagat
     2881 actccgcggc cgtggacgat caatcgcgca acctcgccac gcttcggggc ctgttcaaga
     2941 tccgcatcgg cgagcgcgag ccggtaccga tcgacgaggt cgaacccgtc gaggagatcg
     3001 tcaggcggtt tgcgaccggc gcgatgagct acggctcgat ctcgcaggag gcgcacgaga
     3061 cgctcgcgat cgcgatgaat cgcatcggag gacgctcgaa ctcgggtgag ggcggcgagg
     3121 atccggagcg cgatcatctc gacccgaacg gcgactcgcg gcgcagccgc atccggcagg
     3181 tggcctccgc gcgcttcggc gtgacg
//
LOCUS       DATLFO010000061         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_101138, whole genome shotgun sequence.
ACCESSION   DATLFO010000061 DATLFO010000000
VERSION     DATLFO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101138"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2217)
                     /locus_tag="VM841_02080"
     CDS             complement(<1..>2217)
                     /locus_tag="VM841_02080"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HVL89002.1"
                     /translation="PFVVDEAARLKEQRLAAVEERIDADLAVGRDADVVAELELLAEQ
                     HPLRERLWGQLMTALYRVGRQADALEAYRRARAVLSGELGIEPGDELRRLEAAILRHE
                     LPPADWEPPHNLPTPLTRFVGRERVVADVIGRLERERLVTLTGPGGSGKTRVALEAAR
                     AVLDRFPDGVWMVDLAPVSDPALLAETVADAVGLAERSARPAGAVVEEHLARTTALLL
                     FDNCEHLGAPCGTYVRRLLENSPRVRVLATSREPLAVPGEMIFEVPPLPTGDGDRTGD
                     VLDSEAVRLFLDRAPAAVPPESLPSVARICRELDGLPLAIEIAAAQTRWLSCEGIADR
                     MADRFRLLSYWSRTAVPRHQTLRASLDWSYERLEQHERVVFHRLGVFAGGATLAAAEA
                     VSGPAGDVFSALRRLVDMSLIFTDANLAPQRFRLLETVRQYAQERSAAAGDAEDAASR
                     HAHFFAALAERGLQVIDTPDQARVHEDIDAEHGNIRAALEWAFRAGDAGLVGRIASGM
                     GPYWRLRGYLDEGEMWLTRAVDLCDQESTEVRGNLLGGLGHIQLRRSDVSAARARLEE
                     AVQVLGAAPGSTSVGRWLQQLAYCCSVQGELARAREVIDEALRLAVANGNSAAASHHV
                     GHLAEIALAEGRLDEAEKLFDRALGLVAPPSPSHLVAYLESTARLAIARREFDEAAAR
                     AGEGLARAQELGDVLHIGLNLITLSYVDVRRKEVDRAAERLLEALERVWPVGEVWAT"
BASE COUNT          385 a          796 c          709 g          327 t
ORIGIN      
        1 agtggcccac acctctccca ccggccagac gcgttccagc gcctccagca accgctccgc
       61 tgcccgatct acctccttcc tgcgcacgtc cacgtacgaa agggtgatga ggttcaggcc
      121 gatgtgcagc acgtcgccca gctcctgggc gcgtgcgagc ccttcgcccg cgcgcgcagc
      181 cgcttcgtcg aactcgcgcc gagcgatcgc gaggcgtgcc gtgctctcga gataggcgac
      241 aaggtgactc ggcgaaggcg gcgcgacgag ccccagcgcg cggtcgaaca acttctccgc
      301 ttcgtcgaga cgaccctcgg caagggcgat ctccgcgagg tggccgacat gatgagacgc
      361 ggctgccgaa ttcccgtttg caacggcgag ccgaagcgcc tcatcaatca cctcacgcgc
      421 ccgcgccaac tcgccctgta ccgaacagca gtacgcgagc tgctgtaacc agcgacctac
      481 gctggtggat ccgggggctg caccgaggac ttgaaccgcc tcctccagcc gcgcacgagc
      541 ggccgagaca tcgcttctcc tgagctggat gtgcccgagg cctccaagca gattcccgcg
      601 cacctccgtc gattcttggt cgcaaagatc gaccgcgcgg gtgagccaca tttcgccctc
      661 gtccaggtag ccgcgaaggc gccagtacgg gcccatgccc gacgcgatcc ttcccacgag
      721 tcctgcatct ccggcgcgga acgcccattc gagcgcggcg cggatattgc cgtgctccgc
      781 gtcgatgtcc tcatgaacac gtgcctgatc aggcgtatcg atcacctgaa gacctcgctc
      841 tgcgagagcg gcgaagaaat gcgcgtggcg ggacgcggca tcctccgcgt cgccggctgc
      901 ggccgatcgc tcttgcgcgt actgtcgcac cgtctccaac agacggaaac gctgcggcgc
      961 aaggttcgcg tcggtgaaga tgagggacat gtccaccaag cgccgaagcg cgctgaagac
     1021 gtcaccggcc ggtccggaga ccgcctcggc agccgcgagg gtcgcgccgc ccgcaaatac
     1081 acccaggcga tggaagacaa cgcgctcgtg ctgctccagc ctctcgtacg accaatcgag
     1141 cgacgcccgt agtgtttggt ggcgcggcac cgcggtgcgg ctccagtacg acagaaggcg
     1201 gaaccgatcg gccatgcggt ccgcaattcc ctcgcacgac agccatctcg tttgagcggc
     1261 tgcgatctcg atcgcgagtg gaagaccgtc gagctcccga caaatccgtg caaccgaagg
     1321 aagtgattcc ggcggaacgg cagcaggtgc gcgatctaga aatagccgaa cggcctccga
     1381 gtcgagcacg tcgccggttc ggtcgccgtc acccgtcggc agcggcggga cctcgaagat
     1441 catctcgccc ggaacggcga ggggttcgcg gctcgtagcg agcacgcgaa cgcgcggact
     1501 gttttccaac agccggcgga cgtacgtgcc gcacggcgcg ccgaggtgtt cgcagttgtc
     1561 gaacaggagc agcgcagtcg tgcgcgcgag gtgctcttcc acgacggcgc ccgccggccg
     1621 ggcgctccgc tcagcgagcc ccacggcatc ggcgaccgtc tctgcgagaa gcgcgggatc
     1681 ggacacgggt gcgagatcga ccatccacac cccgtccgga aagcggtcga ggacggccct
     1741 ggccgcctcc agtgcaacgc gcgtcttgcc cgaacctccg ggcccggtca gcgtcacgag
     1801 ccgctcacgc tccaaacgac caatcacgtc ggccacgacc ctctctcgac cgacaaagcg
     1861 ggtcagcggt gtggggaggt tgtgcggcgg ctcccaatcc gccggcggga gctcatgccg
     1921 gaggatcgcg gcttccagcc ggcgcagttc atcgccgggc tcgatcccca gttcgccgct
     1981 gaggacggcg cgggcgcggc ggtaggcctc cagcgcatcg gcttgccgcc cgacgcgata
     2041 caaggccgtc atcagctgtc cccacaggcg ctccctcagc ggatgctgct ccgcaagcaa
     2101 ctccaattcg gcgacgacgt ccgcgtcgcg accgacggcg agatccgcgt cgattcgctc
     2161 ctcgaccgcg gccagccgct gctccttcag gcgagcggct tcgtcgacca cgaacgg
//
LOCUS       DATLFO010000062         3250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_41743, whole genome shotgun sequence.
ACCESSION   DATLFO010000062 DATLFO010000000
VERSION     DATLFO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41743"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VM841_02085"
     CDS             <1..896
                     /locus_tag="VM841_02085"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVL89003.1"
                     /translation="RARDARRGGGAERVPFGAMTEPTQQPDGRASLPDTRTAAAAALF
                     AGAAALALALGPVALTVFVFALAITAAGELYRIVRRGGGALVPLVGFAGIAALFALAY
                     DGAETLLGGAPAVAAAVAAVALVVFVLRGRIEGALPGVAATTGIALAVGVLGAFVVAL
                     RRVDGGFRVTLAFALMVAIGDVTASLAGRRFARRPLAASIGPSKTWEGLAGGTVGVFA
                     AAAIAGALAGGPVTMSRALVLGTLVAIAAPLGDLSTAMIMRDAPRPRMDVFGRGGILD
                     RIDALLFAAPIFYFAYRAMVR"
     gene            907..1602
                     /locus_tag="VM841_02090"
     CDS             907..1602
                     /locus_tag="VM841_02090"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HVL89004.1"
                     /translation="MTRRLLVTAAILGALFWGANLAAEKVAEARIAAQAQKTFGTTSP
                     PEVSIGGFPIILNVLQGSIPTASLEGRGLTVDELAVDVFRIELEGIRASLSDISEGRR
                     IQIERGLAQAEVTSRAITDFVRSRGYRLTVTALPGKIRVSGSVRGATATAEGTPKQVG
                     RVLQFRPDSVMVEGKPLSGAALAEARKALTFEIDLPILPGGARITSIDYREGRIVMIA
                     TLENASLDLSGSS"
     gene            1704..2390
                     /locus_tag="VM841_02095"
     CDS             1704..2390
                     /locus_tag="VM841_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL89005.1"
                     /translation="MALGQSDSRVARALSAPTRAAILDRLRELGPLTAKEVAEHAGVH
                     PNVARGHLDMLVEAGLAAHSWRRNPAGGRPAKAYEAVPAHVEEGTTLVAEMLAALIEE
                     TGHQPEPARRIAEQTGERLGRRARDNDVVLSFDEQITALLRALGEVSGGVRIADRGED
                     WVEFEDVDCPFKGIAAAHPELACSLDKSLKTGVMRALGAEAVVEVVTSVAWGDPSCRE
                     VVRVRGPINP"
     gene            2412..2843
                     /locus_tag="VM841_02100"
     CDS             2412..2843
                     /locus_tag="VM841_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HVL89006.1"
                     /translation="MSQRMIDRNGHRLGAALSAAGLLIAYIADWRPVAPVMTAVFAIG
                     VIFGLRYSPMGATYRALKKTFKLNVPVSPEEEAPPRFAQTMGLIFMAIGTLGFYSLGW
                     GAGAWIPILMVVALQALLGVTGICVGCEMYLIGKRMTTKAG"
     gene            2845..>3250
                     /locus_tag="VM841_02105"
     CDS             2845..>3250
                     /locus_tag="VM841_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVL89007.1"
                     /translation="MIRILVALALIAAVAAFTWWWKRREGRFTEADGRFDRVSLGIGQ
                     FEKPCAAIVEFYADHCAPCKVVEARLTKIAETVCDLKIIKVDAGERLDLADRYNVKRI
                     PTMFVTDEDLKIVWRASGVPTEDAIMKALLGPE"
BASE COUNT          463 a         1075 c         1164 g          548 t
ORIGIN      
        1 cgcgcgcgcg tgacgcgcgc agaggcggcg gcgccgagcg ggtaccattc ggcgcgatga
       61 ccgagccgac gcagcagcca gacggacgcg cttcccttcc cgacacgcga acggccgcag
      121 cggccgcgct gttcgccggg gccgctgcgc tcgcgctggc gctcgggccg gtcgcattga
      181 cggttttcgt gttcgcgctg gcgatcaccg ccgccggcga gctgtatcgc atcgttcgcc
      241 gcggcggcgg cgctctcgtt ccgctcgtgg gattcgcagg catcgccgcc ctgttcgcgc
      301 tcgcctacga cggcgccgag acgcttctcg gtggcgctcc ggccgtggcc gccgcggtcg
      361 cggccgtcgc gctggtggtc ttcgttcttc gcgggcgcat cgagggagcg ctgccggggg
      421 ttgcggccac gaccggaatt gctctcgccg tcggcgttct cggcgcgttc gtcgtggcgc
      481 tgcgtcgcgt ggacggcggc tttcgcgtca cgctcgcgtt cgcgctcatg gtcgcgatcg
      541 gcgatgtgac cgcctcgctc gcggggcgcc ggttcgctcg gcgcccgctc gcggcctcga
      601 tcggaccgag caagacgtgg gaaggactcg cgggcgggac ggtgggggtg ttcgcggccg
      661 ccgccatcgc gggcgcgctc gccggcggtc cggtgacgat gtcacgcgcg ctggtgctcg
      721 gcacgctcgt cgcgatcgcc gcgccgctcg gggatctgtc gacggcgatg atcatgcgag
      781 acgcgccgcg gcctcgcatg gacgtcttcg gtcgcggcgg catcctcgat cgcatcgacg
      841 cgttgctctt cgccgcgccc atcttctact tcgcgtatcg cgcgatggtc cggtgacgag
      901 atgcgattga cccgccggct tctggtcacc gccgccatcc tgggggcgct cttttggggt
      961 gcgaatctcg cggccgagaa ggttgccgaa gcccggatcg ccgcgcaggc ccaaaagacg
     1021 ttcggcacca cgtcgcctcc ggaggtctcg atcggcggct tccccatcat cctcaacgtg
     1081 ctgcaggggt cgatcccgac cgcatcgctc gaaggccggg ggctgacggt tgacgagctc
     1141 gccgtcgatg tgttccgcat cgagctcgaa gggattcggg cgtcgctgtc ggacatcagc
     1201 gaaggccggc ggatccagat cgagcgaggg ctcgcgcagg ccgaggtgac ctcgagggcg
     1261 atcacggact tcgtgcgcag ccgcggctac cggctcaccg tcaccgcgct gccgggcaag
     1321 attcgcgtga gcggttccgt gcgaggggcg accgccacgg cggagggaac acccaagcag
     1381 gtcgggcgag tgcttcagtt ccggcccgac tcggtcatgg tggagggcaa gccgctctcc
     1441 ggagccgcgc tggcggaggc gcgcaaggcc ctgaccttcg agatcgacct gccgatcctt
     1501 cccggcggcg cccgcatcac ctcgatcgat taccgcgagg gcaggatcgt gatgatcgcg
     1561 accctggaga acgccagcct ggacctgtcc ggctcgtcct gaaatatccg atccgaatct
     1621 tccggcaacg ggaattgacc ggaggtcccc ggggttttta tggtatgaac cgtagaaacc
     1681 ctggagcggt gaggagcgag ttcatggcac ttggacagtc ggattcgcga gtcgcgcggg
     1741 cgttgtccgc gccgacccgg gccgcgattc tcgaccggct gcgcgagctc gggccgctga
     1801 cggccaaaga ggtcgccgag cacgccggcg tgcaccccaa cgtcgcgcgg ggccatctgg
     1861 acatgctcgt cgaggccggt ctcgcggcgc actcgtggcg gcgcaatccc gcaggcggtc
     1921 gcccggcgaa ggcctacgag gccgtgcccg ctcacgtcga ggaagggacc acgctcgtcg
     1981 ccgagatgct ggccgcgctg atcgaagaga ccgggcatca gcccgagccg gcgcgccgca
     2041 tcgccgagca gaccggcgag cggctcggcc gccgtgcgcg cgacaacgac gtggtgttga
     2101 gcttcgacga gcagatcacc gcgctgctgc gcgcgctcgg cgaagtgtcg ggcggggtgc
     2161 ggatcgccga ccgcggcgag gactgggtcg aattcgaaga cgttgactgt ccgttcaagg
     2221 gcatcgccgc cgcgcatccc gaactcgcgt gttcgctcga taagtctttg aagacgggtg
     2281 tgatgcgcgc gctgggagcc gaggcggtcg tcgaagtcgt cacgagcgtc gcgtggggcg
     2341 acccgtcgtg ccgcgaggtc gtgcgcgttc gaggcccgat caatccgtaa cacagcgaga
     2401 ggatgacaga cgtgtcgcaa cgtatgatcg accgaaacgg gcaccgcctt ggtgccgcct
     2461 tgtcggccgc cggtttgctg atcgcctata tcgccgattg gcgtccggtc gcgccggtga
     2521 tgaccgccgt gttcgcgatc ggcgtgatct tcgggctgcg gtattcgccg atgggcgcga
     2581 cctaccgcgc gctgaagaag accttcaagc tgaacgtgcc ggtctcgccc gaagaagagg
     2641 cgccgccgcg gttcgcgcag acgatgggct tgatcttcat ggcgatcgga acgctcggct
     2701 tctattcgct cggctggggc gcgggtgcgt ggatcccgat cctgatggtc gtcgcgctgc
     2761 aagcgctgct gggcgtgacc ggaatctgcg tcggatgcga gatgtatctg atcggcaagc
     2821 gcatgaccac gaaggccggc tgagatgatt cgcatcctgg tcgcgctggc gttgatcgcg
     2881 gccgttgccg cgttcacgtg gtggtggaag cgccgtgagg gccgctttac cgaggccgac
     2941 gggcgcttcg accgcgtctc gctgggcatc ggccagttcg aaaagccctg cgccgcgatc
     3001 gtcgagttct acgcggatca ctgcgcgccc tgcaaggttg tcgaggcgcg tctgaccaag
     3061 atcgccgaaa ccgtgtgcga cctgaagatc atcaaggtcg atgcggggga gcgactggac
     3121 ctggccgacc gctacaacgt caagcgcatc ccgaccatgt tcgtcaccga cgaggatctc
     3181 aagattgtct ggcgagccag cggcgtcccc accgaagacg ccatcatgaa ggccctcctc
     3241 ggccccgaat
//
LOCUS       DATLFO010000063        25486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_130417, whole genome shotgun sequence.
ACCESSION   DATLFO010000063 DATLFO010000000
VERSION     DATLFO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130417"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..3471
                     /locus_tag="VM841_02110"
     CDS             79..3471
                     /locus_tag="VM841_02110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVL89008.1"
                     /translation="MNQPAFAHLSVRTVYSLREGAIRPDELAARTAALGMNAVAITDT
                     NGLYGAVRFAQACAQHGVKPIYGTRLTVAGHAGERFVLTLLAANDDGYTNLCRLLTAA
                     HNRGERGDPCATIEDVLDHAHGMFLLLGPESDAQTLLRARRFDAAVAAARRWREGFGR
                     RLVIETRNLLEPDSGLRMQDALRLADEIDAKAVLTNGVRAATPADAFLPDLLDAIRKL
                     VPVSQHAREATNYEATLKSPLEMSLLARGRTDLLENTARIADECTVDIGLGQLHLPEY
                     PAARGFGGLKERSGSPPDQTARSVNELLAKRCHEGLRRRGMDPSDRTVRERLDAELSL
                     TARLGFAGYFLTVADIVASMKRKGIRCQCRGSAAGSLIIYLTGVSEVDPIEHDLLFER
                     FVNPHRTELPDIDIDVESARREEIYDDILKAFPRDRVACVAMVDTYRARGAIRETGKA
                     LGFPDGEVDIVAKAFPQIGARHIRDSLEHLPELKRTSLARGQLDLIFAVAERLAGFPR
                     HLALHPSGILIAPDDLTTRSPLERSAAGFRMVQFDKDDVEALGLLKLDVIGIRMLSTM
                     THAIKEIRRTSGQQVDLDAIPRDDDKTFDLVRASRTLGCFQIESPGQRELLAKFQPDR
                     WKDLIIDISLFRPGPVNSDMIGPYLNRRHGFANPIYAHPALRPFLQQSYGVIVYHEQV
                     MRAIAVATGVDLGKADQIRRELSDPDRLPAIKEWFYDASRERGWNDRDIDAIWKEVAS
                     FAAFGFCKAHAAAFAVPTYQSAWLKANHPAAFYAGVLTHEPGMYPRRAIIDDARHEGV
                     PVFNVDVNVSVREYTVEAGGVRIGLMHVDGISAAEIDSILTARESGGVFRDLGDLCRR
                     TRLSRPTVENLIHTGACDAFGGRRDLLLRVKDLWTKKMPKPERAQTDLKVAEPAPVYG
                     LRAYSDAEKVRAEIEVTGMDVSRHVISFYEPVLGALRVTRSEGLRATRQRQRVMVAGV
                     KVASQTPAVRSGQRIIFLTLDDGTGLVDATVFESVQEHCAWTVFHSYLIVVRGTVHRT
                     GKRGVSINVERAWELGSLAKQYRAGALDVDAMWTEGVAEIEAAERERLRTRGKKAPPV
                     KHSGGQLVLPLPAAGPAANPAEAPAAQAPRKLWHSSGGSAGA"
     gene            3500..4729
                     /gene="dinB"
                     /locus_tag="VM841_02115"
     CDS             3500..4729
                     /gene="dinB"
                     /locus_tag="VM841_02115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVL89009.1"
                     /translation="MSEMTSSPASAEPILHVDMDAFYASVEARDDPTLAGIPLAVGSA
                     GPRGVVAAASYEARVYGVHSAMPSVQARRLCPELVFVPPDFDRYKAESDAVFEIFRSF
                     TPHVEPLSLDEAFLDVAGAERLFGSPESIAHAIKARVRDERRLPCSVGVAPNKFLAKL
                     ASGKAKPDGVLVVRAGAIRAFLDPLDVEDLWGVGEVTATALRRLGARTVAELRALPAG
                     ALERALGANAATHLRSLASGIDDRAVVAREPAKQVGAEETYERDLDDPADVRRELLRL
                     SERVGRRLRAEGMSGRTITIKVRFSTFKTITRARTIEAPTNVAEVLYETARDLYKAAA
                     FDRPRIRLLGISVSSLVEGDGSAQLSLGEERPDRWRAADGAADALRKRFGSGAVERAA
                     LADRKPVRARRPPPPAR"
     gene            4753..5538
                     /locus_tag="VM841_02120"
     CDS             4753..5538
                     /locus_tag="VM841_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL89010.1"
                     /translation="MSEDKVLYEVDDRVAVVTLNRPDRLNAMDADVFRLLTEHARRAA
                     ADPQVRAVVVRGEGRSFSSGLDISLFGQQASEDGVPRIDIESLQRAFTVYEECPKPTV
                     AAVQGHALGGALQLAIACDIRIAATDAVFSAAEVRWGIIPDLGATQRLPRLIGLGRAK
                     EMVLTGRNVTADEAGHWGLVNRICEPDALLKEAMSLASQLAVGPPLALAAAKRLTGSA
                     LDRPVAEGLDREAQAQRTLLASQDFREAVTAGFEKRAPAYQGR"
     gene            5629..5997
                     /locus_tag="VM841_02125"
     CDS             5629..5997
                     /locus_tag="VM841_02125"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HVL89011.1"
                     /translation="MPLSEHEQRILDEIERRLVEEDPKFARGVHAKTPHGQSLRRLRR
                     GIMGFAAGLALLISGLLIPDAFFLFGIAAFAVMLASAVVIASSVKAIGHKPVAPDQKK
                     RGAWFDKMEDRWKKRFEKDE"
     gene            6176..6688
                     /locus_tag="VM841_02130"
     CDS             6176..6688
                     /locus_tag="VM841_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVL89012.1"
                     /translation="MMEGFTALQASRFTGCTGNQLRYWDKIGLVKPSVQPTRGRPGVR
                     RLYSFRDLVSLKVVKSLLDEGMSLQRVRRAYEYLRDAHALEDHLAGIKLVTDGKSIFR
                     VSKGDDEFEDALRKGQMAFFVAIDKMAREVDDGVTSFVRDRDQFIRTIKAAESDLRAE
                     ISPKRRRVAR"
     gene            complement(6714..8918)
                     /locus_tag="VM841_02135"
     CDS             complement(6714..8918)
                     /locus_tag="VM841_02135"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HVL89013.1"
                     /translation="MARLDARVRAPAELVSAALVAAATLPLGRLFHSGRAVPTILVTI
                     AAGTGVAWALRRARIFTPLAALIAAAVFFWHAGLLFHRDAMLGPLPTPAMFAEVGRAI
                     AFAIRASTTEVAPVAATPSLLTLIAMVVWAAAWLADDAIHKLRHPLLAIGFVLPVFVF
                     PATLLASPRRWLDAALFIGASLAVMFTDESSRLSRWGRTAGIGTPGWKPGLAARTGGL
                     VTLAAILAAPLVPGYGAEPGFGTGGEGFGRGDRVALNPLVSIQPSLNQTPAVPLFTVK
                     AEEPAYWRITALDQFNGIVWSAPPRRAVPEISGTTIPATVPVPQSRTLKQEITLAKLS
                     GPWLPGAYEAIRVDRRDSVRLEPVTRSLVLSGQMRTGERVDVTSRLISPAPEDLDRIT
                     AEDITGDPIGSQFLGLPGNEVPAEIRAAALQITDGETSLFRKAVAIQNHLRQFEYDDQ
                     VANGHSYRTMSQFLLEVRRGYCEQFSSTMAVLARVIGIPSRVAIGFAAGDPIGADEYR
                     VTTKHAHAWVELYFPGYGWLPFEPTPRSEITITPTYTTPQALNPTINPEPTTQPTAQP
                     TGSAAPPTSEPRDPDVGGAAGGVAGAPKDRKPLWIALALIAGTLLALPGGAAAKRLLR
                     RRKATNARAATVAHYMDFLDWCAAARVPKRPGETPAEYAGRLAAMAAPAEGPLRDLAA
                     ITERTLWAPAATAEAERAAALGKQARHAVAPSLPAASRLMSMAGWGWWKARS"
     gene            complement(8858..10126)
                     /locus_tag="VM841_02140"
     CDS             complement(8858..10126)
                     /locus_tag="VM841_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HVL89014.1"
                     /translation="MPTRRGWAVIGAAAGCLVAARTTAVKELYMLGIAAALLPLVGGL
                     LVRWGSVNLTIKRRVAARRVFPGVRMRVDLDVRNDGGMRTPPLLVEDDAAPAFGGPAR
                     FSLPSLPAGSRTPVGIERTAAARGRYQIGPLRLRLVDPFGLAERTVIVRDIDRVTVYP
                     KIEPLIGDGPPAERAGAGPASVYRLAPEGDEFYAVREYESGDDLRKIHWRSVARTGQL
                     MIKQEEARFFPRATVFVDTRRIGHHGTGADSSLEWAISAAASTLWHLARQGFALRLAT
                     EELAPLGARSGKEAAESLLEALAMVTPSSHATLQHGLRKVAQRPGADGALLVFMPPPG
                     PEDLRTLARLRTLYSWCGAVLIDADASISSLTPRGRAEADQKIAAAEATLSRAGWKVR
                     SADSKERFRDLWHGLTLASAPRPSSSPQRS"
     gene            complement(10129..11103)
                     /locus_tag="VM841_02145"
     CDS             complement(10129..11103)
                     /locus_tag="VM841_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVL89015.1"
                     /translation="MPPRKSAVPSTVADSATLLGRIRDNIDQVLQGKDEQVRLALVCM
                     LAHGHLLIEDVPGVGKTLLAKSLARTMGCAWRRIQFTPDLLPTDITGVSVYNQERRNF
                     EFKPGAIFANVVLGDEVNRASPKTQSALLEAMEERQATVDGVTYPLPEPFLVIATQNP
                     IEHEGTYPLPAAQLDRFLMRLSIGYPSVEAELQVLHTHGRHSALEDIEAVTDSRAITA
                     LMDIAGRVHVADSLASYIVEIVAVTRASGELTLGASPRASLALLRASRAYALTEGRDY
                     VVPDDIKTLAVPVLAHRVIAAPEAEMSGRTAGDVIEDLLEEVPVPIRG"
     gene            11326..12975
                     /locus_tag="VM841_02150"
     CDS             11326..12975
                     /locus_tag="VM841_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89016.1"
                     /translation="MIHGKAIRLAALVTVLGLVAAACGGDGGDGGTGGDGKSGGTFVF
                     GASADPVSLDGAFVSDGESIRVIYQVFEGLVRTEDGGFKPVPALATEWEPSSDGKTWT
                     FTLREGVKFHDGTDFNAEAVCFNFNRWYNFKGLLQAESLTYYWKNVFGAFSDKPAESL
                     FKSCTAKDPKTVVIELTRASASFLNALVLANFSIASPAALQKYEADKVGGTAESPTLE
                     GSYGLEHPTGTGPFKFVSFAAKDRLVLERNDDYWGEKAKFDRVIFRAIPDAAARRTAL
                     ETGEIQAFDNPSPDDIASLKNNFAIEERPPFNVGYIGFNQKIKPFDNLKIRQAIAHAI
                     NKDALLKAKYPEGAQAAIQFQPPDLFGWNGDVPKYEYDVNKAKQLIAESGLPQPVTLE
                     FWYPTDVSRGYMPDPSANFQAFKSDLEAAGFRVTPKSAPWRPDYLNAAQSGQAGMYLL
                     GWLADFGDPDNFVGVFFQQPSDQFGFDNKAIFDKLTEAEVETDEGKRTDLYKEANKMI
                     MELVPGVPYVHTATFVAMAKKVQGFVPSPINLERFSIMSFA"
     gene            13022..14026
                     /locus_tag="VM841_02155"
     CDS             13022..14026
                     /locus_tag="VM841_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89017.1"
                     /translation="MLKFAVRRLLHLIPILIGLSLLVFIWIRSLPGGPAEALLGERAT
                     PDKVEQIRELYGLDRPVHEQYFSYVKRVASLDFGQSIASRRSVTTEIAERFPATVELA
                     LAAMIFAVGIGIPLGFAAAKRYQTWFDHGSLAGSLLGISIPVFFLAILLKWLFAVKLQ
                     WLPTVGRLDVTRTVEHPTNFYLLDAILTLDAAAFWDAVRHLILPAIALGTIPLAIVTR
                     ITRAAVLDVVNEDYVRTARAKGLSPAMVNRRHVLRNAMLPIVTIVGLQMGLLFSGAVL
                     TETVFAWGGMGSWLASAIFNRDYPVLQGGILFLATMFVLVNLVVDMIYALVDPRIRVG
                     "
     gene            14031..14948
                     /locus_tag="VM841_02160"
     CDS             14031..14948
                     /locus_tag="VM841_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89018.1"
                     /translation="MSIAEAEAREAVTVAFDEDTAGLWREAWRRLRRNPTAIFGMTMI
                     VLFVLVAVFAPVLAPFSPTKVDLTNIRPGSFPGPSAEHWFGLDQLGRDELSRIIYGAR
                     YSLIIGVVSLSIGAAVGMLIGAIAGASPRRWVDSVLMRLMDMMLAVPGLLFAIAIAAT
                     LGRSLTSVMIAIGVVNVPIFARLLRGSMLTQREQDYVLAARAVGVKPRKIVMSHMLPN
                     AIAPVIVQGTLSMATAIIDAAGLAFLGLGSADPGVPEWGRMLAETQSFLQHAPHLALF
                     PGAAIVLSVLGFNLVGDALREALDPKLRR"
     gene            14951..15940
                     /locus_tag="VM841_02165"
     CDS             14951..15940
                     /locus_tag="VM841_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89019.1"
                     /translation="MALLEVRDLHVTFKRRGRPAVAAVDGVSFDVEPGSMTGIVGESG
                     CGKSVTSLAIMGLLPSRGVEASGSVRLDGIELIGLSDEDMRGIRGRRIAMIFQDPMTS
                     LNPVLTIGRQISEAIRRHFGTDDEEARRRAAEILDAVGIPGARKRLGDFPHQFSGGMR
                     QRVVIAMALACEPSLIIADEPTTALDVTIQAQILELIRALVVERGTALIMITHDLGVV
                     ANMCETVNVMYAGRFVETAERYALFAEPRHPYTAGLLASVPRLDEPRGTRLNPIRGNP
                     RDVIPWHTGCAFAPRCDNVIDACTAGTPALAEIGFGRTLRCFNPVPAAPEEHA"
     gene            15937..16968
                     /locus_tag="VM841_02170"
     CDS             15937..16968
                     /locus_tag="VM841_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HVL89020.1"
                     /translation="MSEPLLEVKDLRVYFPIKEGIVVERQVGAVRAVDGVDLEIERGE
                     TLGLVGESGCGKTTVGRAILRLVEPTSGSIVFDGRDITHLKGEELRVLRRRMQTIFQD
                     PFSSLDPRQSVQRIVAEPMRVHGLASRGEVDDRVRELLNTVGLPAEAGGRYPHEFSGG
                     QRQRIGIARALGLNPDLIVADEPVSALDVSIRAQVLNLLEELQERFSLTYLFVSHDLA
                     VVRHISDRIAVMYLGTIVETSSADALYEQPLHPYTLALMSAIPIPDPTVEEHRRRILL
                     SGDLPSPVNPPPGCRFHTRCPFRQPDRCADETPALREIEAGHRVACHYAEQIRDGRIR
                     ADAEAVVEG"
     gene            17115..17594
                     /locus_tag="VM841_02175"
     CDS             17115..17594
                     /locus_tag="VM841_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL89021.1"
                     /translation="MVPIRRAVVLVLKEKAEVVHHNGGALHSERMSFAIPSVIRLKYV
                     VRVPYRARVSLSRRAVFVRDNFTCQYCGRTAENVDHVVPRSKGGTHTWDNVVAACKRC
                     NSTKEDKTPQQAGLKLRSKPSAPRETVWIVVAVGRVDPKWEPYLGRVRDLVAPQSTA"
     gene            17651..18790
                     /locus_tag="VM841_02180"
     CDS             17651..18790
                     /locus_tag="VM841_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89022.1"
                     /translation="MFDRPFWTSRIEQAWERRSLVWLVGVRRSGKTVLSRALDDVEYL
                     DCELPSVRRLLADPESFLRSVRGKRVVLDEVHRLPDPSELLKIAADHFPTARVLATGS
                     STLQATAKFRDSLSGRKEEVWLTPMIELDRRSSGGDLPARLERGGLPAFFLRGGSEQE
                     MQEWLDAYWARDVQELFRLERRSSFVRFVELILARSGGIFEATAFAGPSEVSRQTIAN
                     YLSALEITKVAHVVRPFSTRRATEIVHAPKVYAFDTGFVKTFRGWGEMRDEDLGVLWE
                     HYVLNELHARGGVIDIRHWRSTKHQEVDFVIVRRGRPPLAVECKWRADGREDLTGLKA
                     FRRAYPKGESLVVTSDVERSFQRELLPGVSVRYVSLEEMLRAVQS"
     gene            19802..20224
                     /locus_tag="VM841_02185"
     CDS             19802..20224
                     /locus_tag="VM841_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HVL89023.1"
                     /translation="MSFHGAAQHTIDAKGRVFLPAKFRPQLAVGLVVTAGPGGRLWIF
                     DLPSWEPFVERLNQMRRDGKVDPDAAGMVIELAHDTTADAQGRILLPEVLRDYAKLER
                     DAAFVGQGDRIDVWPATVWERRHQTGTQHLREMTDFPA"
     gene            20245..20430
                     /locus_tag="VM841_02190"
     CDS             20245..20430
                     /locus_tag="VM841_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89024.1"
                     /translation="MTQQERSVGAAAVATVATDAATRPGTVRELADSLLLFGLTVTSL
                     GAYVGIALVAVRLLGTK"
     gene            20435..21370
                     /gene="rsmH"
                     /locus_tag="VM841_02195"
     CDS             20435..21370
                     /gene="rsmH"
                     /locus_tag="VM841_02195"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HVL89025.1"
                     /translation="MHVPVLADAVTALLVPEPTEATIVDATLGAGGYAERILEAMGPN
                     GNVIGIDRDEVALGIARERLARFGERVRYVHAGFDRLEEIVRDGHGGQVDGVVYDFGL
                     SSMHVDDPSRGFSFRHDAPLDMRMDPSQTLTAADVLNTYEETELARIIRTYGEERWAA
                     RIASFIVLHRRRTPISTTLELVEIIRAAVPKAARRGGPHPARRTFQALRIEVNKELEQ
                     IEASLPQALSVTRPGGRLVAVSYHSLEDRIVKRFFRGEADGEAPRLRLIKRKPIRPTE
                     DEIEQNPRASSAMLRAAEVLASPFPKFPGPEGSAA"
     gene            21484..21906
                     /locus_tag="VM841_02200"
     CDS             21484..21906
                     /locus_tag="VM841_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89026.1"
                     /translation="MRDRPAPGRRPPASTMIMAGLATAVALFGMVTVQSLVGQTQLAT
                     TEIQRRVDAKKDALEALEVQVANLASLDRVAARATELGLVVPPAIVFLPAPGDEDLDA
                     GSLPSFDDAIATSDGSDASAPRGPARSGAPTTPEPGGR"
     gene            21919..23808
                     /locus_tag="VM841_02205"
     CDS             21919..23808
                     /locus_tag="VM841_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVL89027.1"
                     /translation="MRRRGARPALRLLALLGACSFGLIAIGGRLVHLQVVKASAYEDL
                     GAKQRIRKMELPARRGAIYDRNGVPLAVSVDARAIYANPQFIDDPEGTAVLIAKHLGT
                     DTETILTRLTKKKSGFVYLARKVDIAAANAIMNLDLPGIGTLDETKRVYPQGTLAGQV
                     LGFVGTDNQGLSGLEATFDEVLAGTPGEQIVEVDPQGRPIASGVRSVREPVRGRDIVL
                     TIDRDVQFQAERAVADAVIKWTAKHGMAVVMDSQSNEILAMANYPPFDPARFGDYPVE
                     TRRNRAVQDAYEPGSVNKLVTAAAAVESGLVRPGEMMTVPYRTFVGKEDFTDFEPHPT
                     WNITYNEVLAKSSNVGTIQVAQKIGGKRLYEMLTKFGFGKRTGVEFGGESPGISLAYE
                     KWSKTSLATISIGHGIAVTPLQMLNVYTTLARDGIWKQPRLVTTVGDDERKPSQQPRR
                     VASTFTTAQLRGMLMNVVENGTGRNARIPGYLVGGKTGTARKPNVGKRGYGSAVFTTF
                     IGMVPVDRPRFVVGVVLDEPSLHMAAATAAPAFQKITSYVLARWGVPPQVVPPEKEYR
                     KSLQPARAPAPPAPPKPAATPATKPTASPVPKPSASPAPERSGSPSPKPSASAAAAPR
                     NGSGP"
     gene            23888..25351
                     /locus_tag="VM841_02210"
     CDS             23888..25351
                     /locus_tag="VM841_02210"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HVL89028.1"
                     /translation="MMTLPQPIALEALARRAGASVRGDAAATVSDLAYDSREVTRGSL
                     YFCIAGARADGHDFAAAAVAAGAVALVCERPLDLDVPQIVVPSVRKAMGPMSAAAFGD
                     PSRAITVAGVTGTNGKTTTTFLIEQCFAALGMTTGLLGTVETHIAGAVEPAGRTTPES
                     VDLQRMLARMRAAGVTGATMEVSSHGLAMGRVDGVRYACATFTNLTQDHLDFHETMED
                     YFAAKAMLFDPSRSGVGAINADDEYGRRLASGTEIEHVTFGLEHEADVRATDVVMGAG
                     GSRFVCVTPAVRVPVSVRLAGPFNVSNALAALATMYALGLDVEKAAAGIEAMPGVPGR
                     FERVDDGEEFTVLVDYAHTPDSVERVLRAAREICRGRLVVVVGCGGDRDRAKRPLMGA
                     SAVALADRAILTSDNPRSEEPRAIIAEMEQGARETGRPYEVEVDRRAAIRAAVRDARP
                     GDVIVIAGKGHESGQEFAGHTVPFDDRAVAREEIAAR"
BASE COUNT         4013 a         8676 c         8809 g         3988 t
ORIGIN      
        1 tgtcctttac actacgagcc caccgccgca atgcattctc gggcttcgcc tcgcgaagtc
       61 cattccggga gaagcgcgat gaaccagccg gccttcgcgc atctgtcggt ccggacggtc
      121 tactcgctcc gcgagggtgc gatccggccg gacgagctgg cggcgcgcac ggcggcgctg
      181 ggcatgaacg cggtcgcgat caccgacacc aacggcctct acggtgcggt ccgcttcgcg
      241 caagcgtgcg cgcagcacgg cgtcaagccg atctacggca cgcgcctgac tgtggccggc
      301 cacgcagggg agcggttcgt gctcacgctg ctcgccgcga acgacgacgg ctacaccaac
      361 ctgtgccggc tgctgaccgc ggcgcacaac cgcggcgagc gcggcgaccc ctgcgcgacg
      421 atcgaggacg tgctcgacca tgcacacggc atgttccttt tgctcggccc cgaaagcgac
      481 gcgcagacgt tgctgcgcgc ccgccgcttc gacgcggcgg tcgcggccgc ccgccgctgg
      541 cgcgaaggct tcggccgccg cctggtgatc gagacccgca acctgctcga gcccgactcg
      601 ggtttgcgca tgcaggacgc gctgcgtctg gcggatgaga tcgacgccaa agcggtgctg
      661 accaacggcg tgcgcgcggc cacgcccgcc gatgcgttcc tgcccgatct gctcgacgcg
      721 atccgcaagc tggttccggt ctcgcagcac gcgcgcgaag ccaccaacta cgaggcgacg
      781 ctgaagtcgc cgctggagat gagcctgctc gcgcgcggcc gcaccgattt gctcgaaaac
      841 accgcgcgca tcgccgacga atgcaccgtg gacatcggcc tgggacagct gcacctgccc
      901 gagtatcccg cggcccgggg gttcgggggg ctgaaggagc gaagcggtag ccccccggat
      961 caaacggctc gctcggtcaa cgagctgctc gccaagcgct gccacgaggg gctgcgccgc
     1021 cgcggcatgg atcccagcga ccgcacggtg cgcgaacgct tggatgccga gctgtcgctg
     1081 accgcacgac tcggcttcgc cggctacttc ctgaccgtcg ccgacatcgt ggcgagcatg
     1141 aagcgcaaag gcatccgctg ccagtgccgc ggctcggccg ccggcagcct gatcatctat
     1201 ctgaccggcg tcagcgaagt cgatccgatc gagcacgacc tgctgttcga acgtttcgtc
     1261 aacccgcatc gaactgagct gcccgacatc gacatcgacg tcgagtccgc gcggcgcgaa
     1321 gagatctacg acgacatcct caaagccttc ccacgcgacc gggtcgcgtg cgtcgcgatg
     1381 gtggacacct atcgcgcgcg cggcgcgatc cgcgagaccg gtaaggcgct gggctttccc
     1441 gacggcgagg tggacatcgt cgccaaggcc ttcccgcaga tcggcgcgcg ccacatccgc
     1501 gattcgctcg agcatcttcc tgagctcaag cgcacctcgc tcgcgcgtgg gcagctagac
     1561 ctgatcttcg ccgtcgccga acggctcgcc ggctttccgc ggcatctggc gctgcacccg
     1621 tccgggatct tgatcgcgcc cgacgatctg acgacgcgat cgccgctcga acgcagcgcc
     1681 gccggattcc gcatggtcca gttcgacaag gatgacgtcg aggcgctcgg ccttttgaag
     1741 ctggacgtga tcggcatccg gatgctgtcc acgatgaccc acgccatcaa agagatccgc
     1801 cgcaccagcg gccagcaggt ggatctggat gcgatcccgc gcgacgacga caagaccttc
     1861 gatctggtgc gcgcctcgcg cacgctcggc tgcttccaga tcgagtcgcc cggccagcgc
     1921 gagctgctcg ccaagttcca gcccgaccgc tggaaggatc tgatcatcga catctcgctg
     1981 ttccggccgg ggccggtgaa ctcggacatg atcgggccgt acctgaaccg gcggcacggc
     2041 ttcgcgaacc cgatctacgc gcatccggcg ctgcggccgt tcctgcagca gtcctacggc
     2101 gtgatcgtct accacgagca ggtgatgcgc gcgatcgcgg tcgcgaccgg ggtggacctg
     2161 ggcaaggccg atcagatccg tcgcgagctg agcgacccgg accggcttcc ggcgatcaag
     2221 gaatggttct acgacgcgtc gcgcgaacgc ggctggaacg atcgcgacat cgacgcgatc
     2281 tggaaagagg tcgcctcgtt cgcggcgttc ggattctgca aggcgcacgc ggccgcgttc
     2341 gcggtgccga cgtatcagtc ggcctggctc aaggccaacc atccggccgc gttctacgcc
     2401 ggcgtgctga cccacgagcc gggcatgtat ccgcggcgcg cgatcatcga cgacgcccgc
     2461 cacgaaggcg ttccggtctt caacgtcgac gtcaacgtct cggtccgcga atacaccgtc
     2521 gaggcgggcg gcgtgcgcat cggcctgatg catgtggacg gcatctcggc cgccgagatc
     2581 gactcgatcc tgacggcgcg ggaaagcggg ggagtgttcc gtgacctcgg cgacctgtgc
     2641 cgtcgcacgc gcctgtcgcg gccgaccgtc gagaacctga tccacaccgg cgcctgcgac
     2701 gcattcggcg gccggcgcga tctgctgctg cgcgtcaaag acctgtggac aaagaagatg
     2761 cccaagcccg agcgcgccca gaccgatctg aaggtcgccg agcccgcgcc ggtctacggc
     2821 ctgcgcgcct acagcgacgc cgagaaggtc cgcgccgaga tcgaggtcac cggcatggac
     2881 gtgtcgcgtc acgtgatctc gttctacgag cccgtgctcg gcgcgctgcg cgtcacccgc
     2941 agcgaagggc tgcgcgcgac ccggcagcgg cagcgggtca tggtcgccgg cgtcaaggtc
     3001 gcctcgcaga ccccggcggt tcggtcgggg cagcggatca tcttcctgac gctcgacgac
     3061 ggcaccggtc tggtggacgc gaccgtgttc gagtccgtgc aagagcattg cgcgtggacg
     3121 gtgttccact cgtatctgat cgtggtgcgc ggcaccgtgc atcgcaccgg aaagcgcggc
     3181 gtgtcgatca acgtcgagcg tgcctgggag ctgggctcgc tcgccaagca gtaccgcgcc
     3241 ggagcgctgg acgtggacgc gatgtggacc gagggcgtcg ccgagatcga agccgccgaa
     3301 cgcgagcggc tccgcacccg cggcaagaag gcgccgccgg tcaagcattc cggcggccag
     3361 ctggtcctgc ctcttcccgc ggccgggccc gccgcaaacc cggcggaggc cccggccgcg
     3421 caggcgccgc gcaagctgtg gcactccagc ggcggctcgg cgggcgcatg agacggcgca
     3481 tgagacggcg catgagacaa tgagcgagat gacctcctcc ccggcgtcgg ccgagccgat
     3541 cctgcacgtg gacatggacg cgttctacgc gtccgtcgaa gcgcgcgacg acccgacgct
     3601 ggcgggcatc ccgcttgcgg tcggcagcgc cggtccgcgc ggcgtggtcg ccgccgcctc
     3661 ctacgaggcg cgcgtctacg gcgtccactc ggcgatgccc agcgtccaag cccggcggct
     3721 gtgcccggag ctcgtcttcg tcccgcccga tttcgaccga tacaaagccg agtccgacgc
     3781 ggtcttcgag atcttccggt ccttcacgcc gcacgtcgag ccgctctcgc tcgacgaggc
     3841 gttcctcgac gtcgccggcg ccgagcggct gttcggctcg cccgagtcga tcgcgcacgc
     3901 gatcaaggcc cgcgtccggg acgagcggcg tctgccctgc tcggtcgggg tcgcaccgaa
     3961 caagtttctc gccaagctcg cctccggcaa ggccaagccc gacggcgtgc tggtcgttcg
     4021 cgcgggcgcg atccgcgcgt tcctggatcc cctcgacgtc gaggatctat ggggggtggg
     4081 agaggtaacc gcgaccgcgc tgcgccggct cggcgcgcgc accgtcgccg agcttcgcgc
     4141 gctgcccgcc ggcgcgctcg agcgggcgct cggcgccaac gcggccacgc acctgcggag
     4201 cctggcgagc ggcatcgacg accgggcggt cgtcgcgcgc gagccggcca agcaggtcgg
     4261 cgccgaggaa acctacgagc gcgacctcga cgaccccgcc gacgttcgcc gcgagctgct
     4321 gcgcctgtcc gagcgcgtcg gacgccgtct tcgcgccgag ggcatgtcgg gccgaaccat
     4381 caccatcaag gtccgcttct cgacgttcaa gacgatcacg cgcgcgcgta ccatagaggc
     4441 gccgaccaac gtcgccgagg tcctttacga aaccgcacgc gacctctaca aggccgcggc
     4501 gttcgaccgg ccacgcatcc gcctgctcgg catctcggtc tcgtcgctgg tcgaaggcga
     4561 cgggagcgcc cagctcagcc tgggcgagga gcggcccgat cggtggcgcg ccgccgacgg
     4621 agcggccgac gcgcttcgca agcgattcgg gtcgggcgcg gtcgagcgag ccgctctcgc
     4681 ggatcgcaag cccgtgcggg cccggcggcc gcccccgccc gcgcggtagg ctgcatccgg
     4741 agggaggcac tcatgtccga ggacaaggtt ctgtacgaag tcgacgatcg cgttgccgtc
     4801 gtcacgctca accggcccga ccgtctcaac gcgatggacg ccgacgtgtt ccggctgctg
     4861 acagagcatg cccgccgtgc cgccgccgat ccgcaggtcc gtgcggtcgt ggtgcgcggg
     4921 gagggccgct cgttctcgag cgggctcgac atctcgctgt tcggccagca ggcctccgag
     4981 gacggagtcc cgaggatcga catcgaatcc ctccagcggg ccttcacggt ttacgaagaa
     5041 tgcccgaagc cgacggtagc ggcggtccag gggcacgcgc tcggcggcgc gttgcagctg
     5101 gccatcgcat gcgacatccg gatcgccgcg accgacgcgg tcttttcggc cgccgaggtc
     5161 cggtggggga tcattccgga cttgggcgcc acgcagcgtc tgccacgcct gatcggattg
     5221 ggacgtgcca aggagatggt cctgaccggt cgcaacgtca ccgccgacga agccggccac
     5281 tggggcctgg tcaaccgcat ctgcgagccg gacgccctgc tcaaagaggc gatgtcgctg
     5341 gcatcgcagc ttgccgtcgg ccctccgctg gccctcgcgg ccgccaaacg gctgaccggc
     5401 tcggcgctgg atcgtccggt cgcggagggt ttggaccgcg aagcgcaggc gcaacgcaca
     5461 ttgctcgcat cccaagattt ccgggaagcg gtcaccgcag gtttcgaaaa gcgtgcgccc
     5521 gcctaccagg gaagataacg gggaacagaa cggctcagga cgaaggacct gttgtccgcc
     5581 tccacggcgg gcccctataa tccgagtcaa gccgaccggg acggaacgat gccgctatcc
     5641 gagcacgagc agagaatcct cgatgagatc gagcgaaggc tcgtcgaaga ggatcccaag
     5701 tttgcgcgcg gcgttcacgc caagacgccg cacgggcagt ctcttcgacg actgcgccgc
     5761 ggcatcatgg gattcgcggc cggtctcgcg ctgctcatct ccggtctgct catccccgac
     5821 gcgttcttcc ttttcggcat cgctgcgttc gccgtgatgc tcgcgtcggc cgtcgtgatc
     5881 gcctcgagcg tcaaggccat cggtcacaag cccgtcgcgc ccgatcagaa aaagcgcggc
     5941 gcgtggttcg acaagatgga agatcgttgg aagaagcgct tcgaaaaaga cgagtagcgc
     6001 tcacgattgt cgacgaatca acgcgccgca cggacggcgc gttcttcgtt ttcgaacatt
     6061 tttcgaaaac atcgggtgaa caccttgcag gaccaatgcc cggtgaatta gaaaaggaac
     6121 ctcgcgcctt cccccgcgct gctcaccccg ttcaaacggt tacggagtcc tgcaaatgat
     6181 ggaaggcttt accgcgctcc aagcttctcg attcacgggc tgcaccggaa accagcttcg
     6241 ctattgggac aagatcgggc tcgtcaagcc gtcggtccag ccgacgcgtg gccgtccggg
     6301 cgtccgccgg ctctactcgt tccgggatct ggtatcgctc aaggtcgtca agtcgctgct
     6361 cgacgaaggg atgtcgctcc agcgagtgcg ccgcgcctac gagtaccttc gcgatgcgca
     6421 cgcgctcgag gatcatctcg ccggcatcaa gctggtgacc gacggcaaga gcatcttccg
     6481 ggtttccaag ggtgatgacg agttcgagga cgcgctgcgt aagggccaga tggccttctt
     6541 cgtcgccatc gacaagatgg cgcgcgaggt ggacgacggc gtgacctcgt tcgtgcggga
     6601 ccgggaccag ttcatccgga ccatcaaggc cgccgagtcg gacttgcgcg ccgagatctc
     6661 gccgaaacgc cgccgcgtgg cccgctgagc cgatccttca cgaagcccgg tccctaggac
     6721 cgggctttcc accatcccca gccggccatc gacatcagcc gggaggcggc gggcagcgag
     6781 ggggcgaccg cgtggcgtgc ttgcttgccg agcgccgcgg cgcgttccgc ctcggccgtc
     6841 gccgccggag cccacaaggt ccgttcggtg atggcggcaa ggtcgcggag cgggccttcg
     6901 gccggggcgg ccatcgccgc cagcctcccg gcgtactccg ccggggtctc cccgggtcgt
     6961 ttcggcaccc gggcggccgc gcaccagtcc aggaagtcca tgtaatgcgc aacggtcgcg
     7021 gcccgggcgt tggtggcctt gcggcgtcgc agcagacgtt tggcggccgc gccgcccggc
     7081 agcgccagca gcgtcccggc gatcaaggcc agcgcgatcc acagcggctt tcggtccttg
     7141 ggcgcgccgg cgacgccgcc ggccgccccg cccacgtccg gatcgcgcgg ctcgctcgtc
     7201 gggggagcgg ccgagccggt cggctgggcc gtcggctgcg tggtcggctc ggggttgatc
     7261 gtcgggttca gggcctgcgg cgtggtgtag gttggggtga tcgtgatctc gctgcggggc
     7321 gtgggctcga acggcagcca gccgtagccc gggaagtaca gctccaccca ggcgtgcgcg
     7381 tgcttggtgg tgacgcggta ctcgtcggcc ccgatcgggt cgccggccgc gaagccgatc
     7441 gccacgcgcg acgggatccc gatcacccgg gccagcaccg ccatcgtgga tgagaactgc
     7501 tcgcagtagc cacgcctgac ctcgagcagg aactggctca tggtccggta cgagtggccg
     7561 ttggcgacct ggtcgtcgta ctcgaactgg cgaaggtggt tctggatcgc gacggctttg
     7621 cggaacagcg aggtctcccc gtcggtgatc tgcaaggccg ccgcccgaat ctcggcagga
     7681 acctcgttgc ccggcaggcc gagaaactgg ctgccgatcg gatcacctgt gatgtcctcg
     7741 gccgtgatgc ggtcgaggtc ctcgggggcc ggcgagatca gccgcgacgt cacgtcgacc
     7801 cgctcgccgg ttcgcatctg gccgctcaga accaaggacc gcgtgacggg ctcgagccgg
     7861 acgctgtccc ggcgatccac gcggatggcc tcgtaggcgc ccggcagcca cgggccggaa
     7921 agcttcgcga gcgtgatctc ctgcttgagc gtgcgcgact gcgggaccgg aaccgtcgcc
     7981 gggatcgtgg tgccgctgat ctcgggaacc gcgcgccgcg gcggcgccga ccacacgatg
     8041 ccgttgaact ggtcgagtgc cgtgatgcgc cagtaggccg gctcctcggc cttgacggtg
     8101 aacagcggaa cggccggcgt ctggttcagg ctcggctgga tcgacaccag cgggttgagc
     8161 gcgacccggt cgccgcggcc gaacccctcg ccgccggtcc cgaagccggg ctcggcaccg
     8221 tagccgggca cgagcggggc ggccaagatc gccgccagcg tgaccagccc gccggtgcgc
     8281 gcggcgaggc cgggcttcca tccgggcgtg ccgatgcccg ccgtgcgacc ccagcgcgac
     8341 aggcgcgagg actcgtcggt gaacatcacc gccaggctcg ccccgatgaa cagcgccgcg
     8401 tcgagccagc ggcgcgggct ggcgagcagc gtcgccggga agacgaacac cggcagcacg
     8461 aagccgatgg cgagcagcgg atggcggagc ttgtggatcg cgtcgtcggc gagccaggcg
     8521 gccgcccaca cgaccatcgc gatcaaggtc agcaggctgg gcgtcgccgc caccggcgcc
     8581 acctcggtgg tcgaggcccg gatcgcgaac gcgatcgcgc ggccgacctc ggcgaacatc
     8641 gcgggcgtgg gcagcggacc gagcatcgcg tcgcgatgga acagcaggcc ggcgtgccag
     8701 aagaagacgg ccgcggcgat cagcgccgcc agcggcgtga agatgcgtgc gcggcgcagc
     8761 gcccacgcga cgccggtgcc ggccgcgatg gtcacgagga tcgtcggaac cgcccgtccc
     8821 gagtggaaca ggcgtcccag gggaagcgtc gccgccgcta cgagcgctgc ggagacgagc
     8881 tcggccgggg cgcggacgcg agcgtcaagc cgtgccatag gtctcggaac ctttccttgg
     8941 agtcggcgga gcggactttc cagccggcgc gggacagcgt cgcctcggcg gcggcgatct
     9001 tctggtcggc ctccgcgcgg ccgcgtggtg tcagcgagct gatcgaggcg tcggcgtcga
     9061 tcagcaccgc cccgcaccac gagtacaagg tgcggagacg cgcgagcgtg cgcagatcct
     9121 cggggccggg cggcggcatg aagaccagca gcgcgccgtc cgcgcccggc cgctgcgcca
     9181 ccttgcgcag gccgtgctgc agcgtggcgt gcgacgacgg cgtgaccatc gccagggcct
     9241 ccagcagcga ctcggccgct tccttgccgc tgcgcgcacc gagcggcgcg agctcctcgg
     9301 tggcgagccg gagcgcgaag ccttggcgcg ccaggtgcca caacgtcgag gccgcggccg
     9361 agatcgccca ctcgaggctc gagtccgcgc cggtgccgtg gtggccgatc cggcgcgtgt
     9421 ccacgaagac ggtggcgcgc gggaagaagc gcgcctcttc ttgcttgatc atgagctggc
     9481 cggtccgggc cacgctgcgc cagtggatct tgcgaaggtc gtcgccggat tcgtattcgc
     9541 gcaccgcata gaactcgtcg ccttcggggg cgaggcggta gaccgaggcg ggccccgcgc
     9601 ccgcccgctc ggccggcggc ccgtcgccga tgagcggctc gatcttggga tagacggtaa
     9661 cgcggtcgat gtcccgcacg atcacggtcc gctcggccag gccgaacgga tcgacgagcc
     9721 gcagcctgag cggaccgatc tgatatcggc cgcgggctgc ggcggtgcgc tcgatcccga
     9781 ccggggtgcg cgacccggcc ggcagcgacg gaagcgagaa ccgcgccggc ccgccgaacg
     9841 cgggtgccgc gtcgtcctcg accagcagcg gcggcgttcg catgccgccg tcgttgcgca
     9901 cgtcaagatc cacgcgcatg cgcacgccgg gaaagacgcg gcgcgccgcg acgcggcgtt
     9961 tgatggtgag attgaccgac ccccagcgaa ccagcaggcc gccgaccagc ggcagcagcg
    10021 ccgccgcgat cccgagcatg tagagctcct tgaccgcggt cgtgcgcgcc gcgaccaagc
    10081 agccggcggc ggctccgatg acggcccatc cgcggcgggt gggcacggtc agcctcgaat
    10141 cgggacggga acctcttcga gcaggtcctc gatcacgtcg ccggccgtcc gtccgctcat
    10201 ctcggcctcg ggcgcggcga tcacgcggtg ggcgagcacg ggcacggcga gcgtcttgat
    10261 gtcgtcgggc accacgtaat cgcggccttc ggtcagcgcg taggcccggg aggcgcgaag
    10321 cagcgccagc gaggcgcgcg ggctggcgcc gagcgtgagc tcacccgagg cgcgcgtgac
    10381 cgcgacgatc tcgacgatgt aggaggcgag gctgtcggcg acgtggacgc ggccggcgat
    10441 gtccatcagc gccgtgatcg cgcgcgaatc ggtgacggcc tcgatgtcct cgagcgccga
    10501 gtggcggccg tgggtgtgca ggacctgcag ttcggcctcg accgacgggt agccgatcga
    10561 caggcgcatc aagaaccggt ccagctgcgc ggccggcagg ggataggtgc cctcgtgctc
    10621 gatcgggttt tgggtggcga tcaccaggaa gggctcgggc agcgggtagg tcacgccgtc
    10681 caccgtggcc tggcgctctt ccatcgcctc gagcaaagcc gactgagttt tggggctggc
    10741 acggttgacc tcgtcgccca ggaccacgtt ggcgaagatc gcgccgggtt tgaactcgaa
    10801 gttgcgacgc tcctggttgt agacgctcac accggtgatg tcggtgggca gcaggtctgg
    10861 ggtgaactgg atccgccgcc aggcgcagcc catcgtgcga gcgagcgact tggcgagcaa
    10921 ggtcttgccg acgcccggca cgtcctcgat caacaggtgt ccgtgggcga gcatgcacac
    10981 cagcgccaga cggacctgct cgtccttgcc ctgaaggacc tggtcgatgt tgtcgcggat
    11041 gcggccgagc agggtggcgc tatcggcgac ggttgacggt acggctgatt tccgtggcgg
    11101 cacgaatccc tcctgcagag gtaagacgtc agcatacccg caggcgttcc ctgcggcagg
    11161 tcctccgaat aggtcatacg gcgcgggtgg cccgaaagac ggtcccgccg gcttcccgcc
    11221 ccgaccgtag gaaatactgg caggaggggt agaatcggcc cccgcgggga tcgacgacgt
    11281 ggccggcccc ggaatccgat atccggtcac aggaggtggg agaccttgat tcacggcaag
    11341 gcaattcgcc tggcggcgct cgtgacggtc ttgggtctcg tcgctgccgc atgcgggggc
    11401 gacggcggag acggaggcac cggcggggac ggcaagtccg gcgggacgtt cgtcttcggc
    11461 gcctcggcgg acccggtctc gctcgacggt gcgttcgttt ccgacggcga gtcgatccgt
    11521 gtcatctatc aggtgttcga aggtctggtc cgcaccgagg acggcgggtt caagccggtc
    11581 ccggcgctgg cgaccgagtg ggagccgtcc tcggacggca agacctggac gttcacgttg
    11641 cgcgagggcg tgaagttcca cgacggcacc gacttcaacg ccgaggccgt ctgcttcaac
    11701 ttcaatcgct ggtacaactt caagggcctt ctgcaggccg agtcgctgac ctactactgg
    11761 aagaacgtct tcggggcgtt cagcgacaag ccggcggaga gcctcttcaa gtcctgcacc
    11821 gcgaaggatc ccaagacggt cgtgatcgag ctgacgcgtg cgtcggcgtc gttcctgaac
    11881 gcgctcgtgc tcgccaactt ctccatcgcc agccccgcgg cgctgcagaa gtacgaagcc
    11941 gacaaggtcg gcggcaccgc ggagtccccc acgctcgagg gaagctacgg cctcgagcac
    12001 ccgaccggca ccggtccgtt caagttcgtg tccttcgcgg ccaaggaccg gctcgtgctc
    12061 gagcgaaacg acgactactg gggcgagaag gccaagttcg accgcgtcat cttccgggcg
    12121 atcccggacg cggccgcacg gcgcacggcc ttggagacgg gtgagatcca ggcattcgac
    12181 aacccgtccc ccgacgacat cgcctcgctg aagaacaact tcgccatcga ggagcggccg
    12241 ccgttcaacg tcggctacat cggcttcaac cagaagatca agccgttcga caacctgaag
    12301 atccgtcagg cgattgcgca cgcgatcaac aaggacgcgc tgctcaaggc gaagtacccc
    12361 gagggcgcgc aagccgccat ccagttccag ccgccggacc tgttcggttg gaacggcgac
    12421 gtgcccaagt acgagtacga cgtcaacaag gccaagcagc tcatcgccga gtcggggctg
    12481 ccgcagccgg tgacgctcga gttctggtat ccgaccgacg tcagccgcgg ctacatgccg
    12541 gacccgtccg ccaacttcca ggcgttcaag tccgatctcg aagccgccgg gttccgggtc
    12601 acgcccaaga gcgccccgtg gcgtcccgac tacctcaacg cggcgcagtc cggccaggcc
    12661 ggcatgtacc tgctgggctg gctcgccgac ttcggcgatc cggacaactt cgtcggggtc
    12721 ttcttccagc agccgtcgga tcagttcggc ttcgacaaca aggcgatctt cgacaagctg
    12781 accgaagccg aggttgagac cgacgagggc aagcgcaccg atctctacaa ggaagcgaac
    12841 aagatgatca tggagctcgt cccgggcgtg ccctacgtgc acaccgcgac cttcgtggcg
    12901 atggccaaga aggtgcaggg cttcgttccg agcccgatca acctggagcg cttctcgatc
    12961 atgagcttcg cctaagggat ccgaaccggg ggcggccgcg aggccgcccc caccatcttt
    13021 catgctcaag ttcgcggttc gaaggctgct ccatctgatc ccgatcctca tcgggctctc
    13081 gctgctcgta ttcatctgga tccgctcgct tccgggcggc ccggccgagg cgctgctcgg
    13141 cgagcgggcc acgccggaca aggtcgagca gatccgcgag ctctacggcc tggaccggcc
    13201 ggtgcacgag caatacttct cctacgtcaa gcgcgtcgcg tcgctcgact tcggccagtc
    13261 gatcgccagc cgccgcagcg tcaccaccga gatcgccgag cgctttcccg cgacggtcga
    13321 gctcgcgctc gcggcgatga tcttcgcggt gggcatcggc attccgctcg ggttcgcggc
    13381 cgcgaagcgg tatcagacct ggttcgacca cggctcgctc gccggctcgc tgctcgggat
    13441 ctcgatcccc gtgttcttcc tggcgatcct gttgaagtgg ctgttcgcgg tcaagctgca
    13501 atggcttccg acggtggggc ggctcgacgt cacccgcacc gtcgagcatc cgacgaactt
    13561 ctatttgctc gatgcgatcc tgacgctcga cgcggccgcg ttctgggatg cggtccgtca
    13621 tctgatcctg ccggcgatcg cgctcggcac cattccgctc gcgatcgtga cgcgcatcac
    13681 gcgtgccgcc gtcctcgacg tcgtcaacga ggactacgtg cgcaccgccc gcgccaaggg
    13741 attgtcgccc gcgatggtga accggcggca cgtgctccgc aacgcgatgc tgccgatcgt
    13801 gaccatcgtc gggctccaga tggggctgct gttcagcggc gcggtgctga ccgagacggt
    13861 cttcgcgtgg ggcggcatgg gctcgtggct cgccagcgcg atcttcaacc gcgactaccc
    13921 ggtgctgcag ggcgggatcc tgttcctggc caccatgttc gttctggtga acctggtcgt
    13981 ggacatgatc tacgcgctcg tcgatccacg gattcgggtg gggtgagccg atgagcatcg
    14041 cggaagccga agcgcgcgag gccgtcaccg tcgccttcga cgaggacacc gccggcctgt
    14101 ggcgggaggc gtggcgccgg ctccggcgca acccgacggc gatcttcggc atgaccatga
    14161 tcgtgctctt cgtgctcgtg gcggtgttcg ctccggtgct ggcgccgttc agcccgacga
    14221 aggtggatct gaccaacatc cgtcccggct cgttccccgg cccgtcggcc gagcattggt
    14281 tcgggctgga ccagctcggc cgcgacgagc tgtcgcgcat catctacggc gcccgctact
    14341 cgctgatcat cggcgtggtc tcactttcga tcggtgcggc cgtcggcatg ctgatcggcg
    14401 cgatcgccgg cgcctcgccg cggcgctggg tggactcggt gctgatgcgg ctgatggaca
    14461 tgatgctcgc ggtgcccggg ctgctgttcg cgatcgcgat cgccgcgacg ctcggccgct
    14521 cgctcacgtc ggtgatgatc gccatcggcg tggtgaacgt gcccatcttc gcgcggctgc
    14581 tgcgcggctc gatgctcacc cagcgcgagc aagactacgt gctcgccgcg cgcgcggtcg
    14641 gcgtcaagcc gcgcaaaatc gtgatgagcc acatgcttcc gaacgcgatc gccccggtga
    14701 tcgtgcaagg cacgctgtcg atggcgacgg cgatcatcga cgcggcgggg cttgcgttcc
    14761 tcgggctcgg ctcggccgat ccgggcgtgc ccgagtgggg ccgcatgctc gccgagacgc
    14821 agtcgttctt gcagcacgcc ccgcatctgg cgctgttccc gggcgcggcg atcgtgctgt
    14881 cggtgctcgg cttcaacctg gtgggcgacg ccctgcgcga ggccctcgac ccgaaactga
    14941 ggcggtagcg gtggcactgc tcgaggtccg ggaccttcac gtgacgttca agcggcgcgg
    15001 ccgtccggcc gtcgcggcgg tcgacggcgt ctccttcgac gtcgagcccg gcagcatgac
    15061 cggcatcgtc ggcgagtccg gctgcggcaa gagcgtcacc tcgctcgcga tcatgggcct
    15121 gctgccgagc cgcggcgtcg aggcgtccgg ctcggtgcgg ctggacggca tcgagctgat
    15181 cgggctgagc gacgaggaca tgcgcggcat ccgcggccgg cgcatcgcga tgatcttcca
    15241 ggatccgatg acctcgctga atccggtgct gaccatcggc cgccagatca gcgaggcgat
    15301 ccgccgccac ttcggcaccg acgacgagga ggcgcgccgc cgcgctgccg agatcctcga
    15361 cgcggtcggc atccccggcg cacgcaagcg gctgggcgac tttccccatc agttctccgg
    15421 cggcatgcgg cagcgcgtcg tgatcgcgat ggcgctcgcg tgcgagccgt cgctgatcat
    15481 cgccgacgag cccacgaccg ccctcgacgt caccatccag gcgcagatcc tcgaactgat
    15541 ccgcgcgttg gtcgtcgagc gcggcaccgc gctgatcatg atcacgcacg acctgggtgt
    15601 cgtggcgaac atgtgcgaga ccgtgaacgt gatgtacgcg ggccggttcg tcgaaaccgc
    15661 cgagcgctat gcgcttttcg ccgagcctcg ccatccctac accgccgggc tgctggcgtc
    15721 ggtgccgcgc ctggacgagc cccgcggcac gcgtctgaac ccgatccgag gcaacccgcg
    15781 cgacgtgatc ccgtggcaca ccggttgcgc gttcgcgccg cgatgcgaca acgtgatcga
    15841 tgcctgcacg gccgggacac ccgcgctcgc agagatcggc ttcggccgga cgctgcgctg
    15901 cttcaacccc gttccggcgg cgccggagga gcacgcgtga gcgagccgct gctggaggtc
    15961 aaggatcttc gcgtctactt cccgatcaag gaaggcatcg tcgtcgagcg gcaggttggc
    16021 gccgtgcgcg ccgtcgacgg cgtggacctg gagatcgaac gcggcgagac gctcggcctg
    16081 gtgggggagt cgggttgcgg caagaccacg gtgggccggg cgatcctgcg gctggtggag
    16141 ccgacgtcgg gctcgatcgt cttcgacggc cgcgacatca cgcacttgaa gggtgaggag
    16201 cttcgggtgc tgcgccggcg catgcagacg atcttccagg acccgttctc gtcgctggat
    16261 ccgcgtcaga gcgtgcagcg catcgtggca gagccgatgc gtgtgcacgg gctcgcctca
    16321 cgcggcgagg tcgacgatcg cgtccgcgag ctgctgaata cggttgggct gccggcggag
    16381 gcgggcggcc gctacccgca cgagttctcc ggcgggcaac gtcagcgaat cgggatcgcg
    16441 cgtgcgctcg ggctgaaccc ggatctgatc gtggccgacg agccggtctc cgcgctggac
    16501 gtgtcgattc gcgcgcaggt gctcaacctc ctcgaggagc ttcaggagcg gttctcgctg
    16561 acctatctgt tcgtgtcgca cgatctcgcg gtcgtgcgac acatctcgga ccggatcgcg
    16621 gtgatgtacc tgggcacgat cgtcgagacc tcctcggccg acgcgctcta cgagcagccg
    16681 ctgcatccct acacgctcgc gttgatgtcg gcgatcccga ttcccgatcc caccgttgag
    16741 gagcatcgcc gccgcatcct gctctccggc gacctgccct cgcccgtcaa cccgccgccg
    16801 ggatgccgct tccacacccg atgcccgttc cgtcagcccg atcggtgcgc cgacgagacc
    16861 ccggcgctgc gcgagatcga ggccggacac cgggtggcct gtcactacgc cgaacagatt
    16921 cgggacggcc ggatccgggc cgacgcggaa gccgtcgtcg agggataggt tcggcttcgg
    16981 ccccgttttc ccgacgggat cggcccgatt cccgctgcta tactccgcga cgagccggcc
    17041 accccggccg gtaaggaggc cgtacccgct tggggagagc actcgtcctc aacgcgacgt
    17101 atgaaccgct gtgcgtggtt cccatccggc gggcggtcgt cctcgtcctg aaagagaagg
    17161 ccgaagtcgt ccaccacaac ggcggcgccc ttcactccga gcgaatgagc ttcgcgatcc
    17221 cgtcggtcat tcgcctgaaa tacgtcgtgc gcgtccccta tcgggcgcgc gtttctttat
    17281 cgcgccgcgc ggtgttcgtc cgcgacaact tcacctgcca atactgtgga cgcaccgcgg
    17341 aaaatgtcga tcacgtcgtg ccgcgctcga aaggcgggac gcacacgtgg gacaacgtgg
    17401 ttgcggcctg caagcgctgc aactcgacca aagaagacaa gacgccgcag caggccggct
    17461 tgaagctgcg gtcaaagccc tcggccccgc gcgaaaccgt ctggatcgtc gtcgccgtcg
    17521 gccgcgtcga tcccaaatgg gagccctact taggacgggt ccgcgacctg gtcgcccccc
    17581 agtcgactgc ctagcggtag gattcgtaac caggattgca gatagtgcca ggatatgcca
    17641 tcctggttgc atgttcgacc ggccattttg gacatcccga atcgagcaag cctgggagcg
    17701 acgctcgctg gtgtggctgg tgggggtgcg gcggtccggc aaaacggtgc tgagccgcgc
    17761 gctggacgat gtcgaatacc tcgattgcga gctgcccagc gtgcggcgcc tgctcgccga
    17821 tcccgagtcg ttcttgcgct cggttcgagg aaagcgggtc gtgctggacg aggtgcatcg
    17881 actgcccgat ccctcggagc tgctcaagat cgcagccgac cactttccca cggcgcgcgt
    17941 gctcgcgacc ggctcatcga cgctgcaggc gaccgcgaag tttcgcgact cgctcagcgg
    18001 acgcaaggaa gaggtgtggc tgacgccgat gatcgagctc gaccgccgca gttcgggcgg
    18061 cgatttgccg gccaggctgg agcgcggcgg acttcccgcg ttcttcctgc ggggcgggtc
    18121 ggagcaggaa atgcaagagt ggctcgatgc gtactgggcg cgcgatgtgc aggagctgtt
    18181 tcgattggag cggcggtcct cgttcgtgcg gttcgttgag ctgatccttg ctcgaagcgg
    18241 cggcatcttc gaagcgacgg cgttcgcagg accatccgag gtcagccggc aaacgatcgc
    18301 caactatctc tccgcgctgg agatcacgaa agttgcccat gtagtgcggc cgttttcaac
    18361 gcgccgggca accgagatcg ttcatgcacc caaggtctat gcgttcgaca ccgggttcgt
    18421 gaagacgttt cgcggatggg gagagatgcg cgacgaggac ctgggcgtgc tctgggagca
    18481 ttatgttctg aacgaactgc atgcgcgggg cggggtcatc gacatcaggc attggcgctc
    18541 gacgaagcat caagaggtcg acttcgtgat cgtgcggcgc ggacggccgc cgctggcggt
    18601 cgaatgcaaa tggcgcgccg acggccgtga agatctcacc ggcctcaagg catttcgtcg
    18661 cgcctacccg aagggcgaaa gcctcgtggt gacctccgac gtcgaacggt ccttccagcg
    18721 cgagctcttg cccggcgttt cggtccggta cgtgagcctt gaagaaatgc ttcgggcggt
    18781 gcagtcgtaa ttggataggt atgtcgatcc cgtagccagg gcgatgtggg actggtgcgt
    18841 cgcgtcgagt acgaatcgcg gcggctcggg ccggagatcc ttgtcgacga tccgattcgt
    18901 agcgtcgctc gggggaaccg gggggcagta tgggccgcgg gaacgactcg cctgtaccgg
    18961 atcaacgaag ccgatcgggc tgtcaccgag gtcgtcgtgc caggcaatga cacgttcctt
    19021 tggctcgact ccgacgggga ctggttgtgg gctctcgggt ggaagcaatt gagcgatcct
    19081 gatgagaacg gaacgatcgc ctcggaatat cgtctgctac ggattgacct ggaaaccggg
    19141 gtcgtgacga gaaccgccag atttcccaag ggggtcctgc gaatcgcagt gacggatcga
    19201 tccgtgtggg ggattggcgg ggccgccatc ccctcgcgcg ggcccgctcc gacatccgcg
    19261 acatctgcgt tatccgatcg aatgcccagg gcgtatgcgg gctatgcatt ccagatcaac
    19321 aaggaatccc ttagcgtcgt tcgcgagatc aatcctgggc gaaccgtgca gacccttcgt
    19381 cttccccggt ccgtccgcga ttcgcagcac gtccggatcg cttcgatagc agcacgttga
    19441 gcgctcgccc catgccgaga aggacgacta cgttcgcgtc gcgccacggc gtatagcggg
    19501 gcgcctcacc ccggacggtg aaacgagcga cccgggtcgc caacgattcc gctgatcatt
    19561 tccgtgttac gagactctta gagagtcatg tgttgtattt gtgacgttct gtggtcgtga
    19621 atgcgaacaa acgttcggaa ctttgtgaac atcggccacc gagagcgcat tgacatcaga
    19681 agagagcggg cgtatcttcg gggagcattg tggtgggaag tggtggacgg tggagccaga
    19741 tgccagccga agccccttcc gcgagggctc cctctgaagg agcggatcga aggatgagaa
    19801 cgtgtcgttt cacggcgccg cgcagcacac gatcgacgcg aaagggaggg tgttcctccc
    19861 cgcgaagttc cggccgcagc ttgcggtcgg gctcgtggtc acggcggggc cgggcggccg
    19921 tctttggatc ttcgatctgc cgtcgtggga accgttcgtc gaaaggctga accagatgcg
    19981 ccgtgacgga aaggtcgatc ccgacgcggc gggcatggtc atcgagctgg cacacgacac
    20041 caccgcggat gcgcaaggac ggatcttgtt gcccgaggtg ctccgcgact acgcgaagtt
    20101 ggagcgggac gcggcgttcg tcgggcaagg ggatcgcatc gatgtgtggc cggcaacggt
    20161 ttgggagcgt cgtcatcaga cgggaacgca gcatctgcgc gagatgacgg acttccccgc
    20221 ctgaacgaga aaggtgaacc aagcatgacg cagcaggagc ggtcggtggg agcagcggcg
    20281 gttgcgacgg tggcgacgga tgcggcaacg cgtccgggga ccgtccgtga gctcgccgat
    20341 tcgctcctgc tgttcggcct caccgtaacg agtctcggcg cctacgtcgg cattgcgctc
    20401 gtggcggtgc ggctgctcgg aaccaagtag ggccgtgcac gtcccggtgc tggcggacgc
    20461 cgttacggcg ctgctggtcc cggagcccac ggaagcaacg atcgttgatg cgacgctcgg
    20521 cgcgggtggc tatgcggaac ggatcctcga ggcgatggga ccgaacggaa acgtcatcgg
    20581 catcgaccgc gacgaggtgg cattgggaat cgcgcgggaa cggctcgcgc ggttcggcga
    20641 acgagtgaga tacgtgcacg caggattcga caggctggag gagatcgtgc gggacggaca
    20701 cggagggcag gtggacgggg tcgtgtacga cttcggcttg tcctcgatgc acgtggacga
    20761 tccgtcgcgc ggcttctcgt tccgtcacga cgctccgctc gacatgcgta tggacccgtc
    20821 gcagactctc actgccgccg acgtcctcaa cacctacgaa gagaccgagc tcgcccggat
    20881 catccgcacc tacggtgagg agcgctgggc cgctcgcatc gcaagcttca tcgtcctcca
    20941 ccgccgccgt accccgatct cgacaaccct cgagctcgtc gagatcatcc gagcggcggt
    21001 gcccaaagcg gcgcgccggg gcgggccgca cccggcgcgc cgcacttttc aagccctgcg
    21061 catcgaggtc aacaaagagc tcgagcagat cgaagcttcc ctaccgcagg cgctctccgt
    21121 caccagacct ggcgggcgtc tcgtcgccgt ttcgtaccat tcgctggagg atcgcatcgt
    21181 caagcggttc ttccgcggcg aagccgacgg cgaggcgccg cgcctgcggc tgatcaagcg
    21241 caagcccatc cgccccaccg aagacgagat cgagcagaac ccgcgagcgt cgtcggcgat
    21301 gttgcgcgcc gccgaagtgc tcgcgtcgcc gttcccgaag tttcccggcc ccgaggggag
    21361 cgcggcatga cgccgcccgc cgacgccgcg gccgctcgca aggcccgcgc gaccaccgcg
    21421 cagccacaga taagccccag gcgaccggcc ccggcgccgg cccgaccgcg ccaccttcgg
    21481 gtggtgcgcg atcggccggc gccgggccgg cgcccccctg cgtccaccat gatcatggcc
    21541 gggctggcga ccgcggtcgc gctgttcggc atggtcacgg ttcagtcgct cgtcggacag
    21601 acacagctcg ccacgaccga gatccagcgc cgcgtcgacg ccaaaaagga cgcgctcgag
    21661 gcgcttgagg ttcaggtcgc gaacttggct tccctggatc gtgtcgcggc acgcgcaacc
    21721 gagctgggac tcgtcgtgcc gcccgcgatc gtcttcctgc cggctcccgg ggatgaggac
    21781 ctcgatgccg gctcgcttcc gtcgttcgac gacgcgatcg ccacgtcgga cggatccgat
    21841 gcttccgcgc cgcgcggccc agcgcgttcg ggggccccga ccacccccga gccgggggga
    21901 cggtaggaga ccgcgcgcgt gcgacgccga ggcgccagac ccgcgctccg tctgctcgcg
    21961 ctgctcggcg cctgctcctt cggcctgatc gcgatcggcg gacggctcgt gcatctgcag
    22021 gtcgtcaagg cgtccgccta cgaggacctc ggggcgaagc agcgaattcg caagatggag
    22081 ctgcccgcgc gtcgcggcgc gatctacgat cgtaacgggg ttccgctggc ggtgtcggta
    22141 gatgcgcgcg cgatctacgc gaacccgcag ttcatcgacg atcccgaggg caccgcggtg
    22201 ctgatcgcca agcatctcgg caccgacacc gagacgatcc tgacgcggct gaccaagaag
    22261 aagagcggct tcgtgtacct cgcccgcaag gtcgatatcg ccgcggcgaa cgcgatcatg
    22321 aatctggacc ttccgggcat cggcacgctc gacgagacca agcgtgtgta tccgcaggga
    22381 acgctcgcgg gacaggtgct cggcttcgtc ggcaccgaca atcagggcct gtccgggctc
    22441 gaggccacct tcgacgaggt gctggcggga acgcccggcg agcagatcgt cgaggtcgat
    22501 ccgcaaggcc gtccgatcgc cagcggcgtg cgatcggtcc gtgagccggt ccgcggacgc
    22561 gacatcgtgc tgaccatcga ccgggacgtg cagttccagg ccgagcgcgc cgtcgccgac
    22621 gcggtgatca aatggacggc gaagcacggc atggcggtgg tgatggattc gcagtcgaac
    22681 gagatcctcg cgatggcgaa ctatccgccc ttcgacccgg cccgtttcgg cgactatccg
    22741 gtcgaaacgc gacgcaaccg cgcggtgcag gacgcctacg agccgggctc ggtgaacaag
    22801 ctcgtcacgg cggcggcggc ggtcgagtcc ggcctcgtgc gccccggcga gatgatgacg
    22861 gtcccgtatc gcacgttcgt cggcaaggag gacttcaccg acttcgagcc gcacccgacc
    22921 tggaacatca cctacaacga ggtgctcgcg aagtcgtcga acgtcggcac catccaggtc
    22981 gcgcagaaga tcggtggcaa gcgtctctac gagatgctga ccaagttcgg cttcggcaaa
    23041 cggaccgggg tcgagttcgg cggcgagtcg ccgggcatct cgctggcata cgagaagtgg
    23101 tccaagacct cgctggcgac gatctcgatc gggcacggaa tcgcggtcac gcctctgcag
    23161 atgctcaacg tctacaccac gctcgcacgc gacgggattt ggaagcagcc gcggctcgtg
    23221 accaccgtcg gcgacgacga gcgtaagcct tcgcagcagc cgcgtcgcgt cgcctccacc
    23281 ttcaccaccg cgcagcttcg cggaatgctg atgaacgtcg tcgagaacgg caccggccgc
    23341 aacgcgcgca tccccggcta tctggtcggc ggcaagaccg gaactgcccg caagcccaac
    23401 gtcggcaagc gcggatacgg cagcgcggtc ttcaccacct tcatcggcat ggttccggtg
    23461 gacaggcccc gcttcgtcgt cggcgtggtg ctcgacgagc cgtcgctgca catggctgcg
    23521 gcgaccgccg cgcccgcctt tcagaagatc acctcgtacg ttctggcgcg atggggcgtg
    23581 cccccgcagg tcgtgccgcc cgagaaggaa taccgaaaat cgttgcagcc cgcccgcgcg
    23641 cccgcgccgc cggcgccgcc caagcccgcg gcaacgccgg cgaccaagcc gacggcttcg
    23701 cccgtgccga aaccgtcggc ctcgcccgcg cccgagcgtt ccggatcgcc gtcaccgaag
    23761 ccgtccgcga gcgccgcagc agccccgaga aacggctcgg gcccgtgata gactcgcccg
    23821 cggcgccggc cggagcgccg atccggcggc acgggtgggg gcgccgcacg gcattgaaga
    23881 cgcgacgatg atgacgctcc cccaaccgat cgcactcgaa gcgctcgccc ggcgggcagg
    23941 cgcatccgtc cgcggcgacg ccgccgcgac tgtgtcggac ctcgcctacg attcgcgcga
    24001 ggtcacgcgc ggctcgctct atttctgcat cgccggcgcg cgcgcggacg gtcacgactt
    24061 cgcggccgcc gccgttgccg cgggcgcggt tgcgctcgtg tgcgagcggc cgctcgacct
    24121 cgacgttccc cagatcgtgg tgccgtcggt ccgcaaggcg atgggaccga tgagcgcggc
    24181 cgcgttcggc gatccgtccc gcgcgatcac cgtcgcgggt gtgaccggca cgaacggcaa
    24241 gacgaccacc acgtttctga tcgagcagtg cttcgcggcg ctcggcatga caaccggtct
    24301 tctcggcacg gtcgagacgc acatcgccgg cgcggtcgag ccggccggcc gcaccacgcc
    24361 cgagtcggtg gacctgcagc gcatgctcgc gcgcatgcgc gccgcgggcg tgaccggcgc
    24421 cacgatggag gtgtcgagcc acgggctcgc gatgggccgt gtggacggcg tccggtacgc
    24481 gtgcgcgacg ttcacaaacc tgacccagga tcatctggac ttccacgaga cgatggagga
    24541 ctacttcgcc gccaaagcga tgctgttcga cccgtcccgg tccggcgtcg gggcgatcaa
    24601 cgccgacgat gaatacggcc ggcgccttgc gtccgggacc gagatcgagc acgtcacgtt
    24661 cggccttgag cacgaggcgg acgtgcgtgc gacggacgtg gtcatggggg ccggcggctc
    24721 gcgcttcgtg tgcgtgaccc cggcggtgcg ggtgccggtg tccgtgcgcc tcgccgggcc
    24781 gttcaacgtc tccaatgcgc tcgcggcgct ggccacgatg tacgcgctcg gcctcgacgt
    24841 cgagaaggcc gcggccggca tcgaggccat gcccggcgtt cccggccgtt tcgagcgggt
    24901 cgacgacggc gaggagttca cggtgctcgt cgactacgcg cacactcccg attcggtcga
    24961 gcgcgtcctg cgcgctgcgc gcgagatctg ccggggacgg ctggtggtcg tcgtcggctg
    25021 cggcggcgac cgcgaccggg ccaagcgtcc tttgatgggc gcgtccgcgg tggcgctcgc
    25081 ggatcgcgcg atcttgacca gcgacaaccc gcgcagcgag gagccgcggg cgatcatcgc
    25141 cgagatggag cagggcgcgc gcgagaccgg ccgcccctac gaggtcgagg tggaccggcg
    25201 cgccgcgatc cgcgccgccg tgcgcgacgc gcgccccggc gacgtgatcg tcatcgcggg
    25261 aaagggccac gaaagcggtc aggagttcgc cggccacacg gttcccttcg acgaccgggc
    25321 cgtcgcccgc gaggagatcg cggcccgatg atgccgatga cgctcgagca gatcgccgct
    25381 gcctgcgacg gacgcgtcga gggcgacgcc gccgtcgtcg tgcgcgacgt cgccaccgat
    25441 acgcgctcga tgccgtccgg cgcgttgttc gtcgcgctgc gggggg
//
LOCUS       DATLFO010000064         6804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_139905, whole genome shotgun sequence.
ACCESSION   DATLFO010000064 DATLFO010000000
VERSION     DATLFO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139905"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VM841_02215"
     CDS             complement(<1..198)
                     /locus_tag="VM841_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89029.1"
                     /translation="MIAQSIVTDETPGRLLLGAVLGATTGYVAGGILGRSVATLIGLA
                     ERQLARLPGADLVAGTLGGVMG"
     gene            197..769
                     /locus_tag="VM841_02220"
     CDS             197..769
                     /locus_tag="VM841_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HVL89030.1"
                     /translation="MDPRLLECVRSGFLRGTMLVGQFVLDEVQAIADSADQVRRNRGK
                     RGLEMLSALHREGLADLRVVDKMYPEFAEVDAKVVALARERGGSIVTNDVALGRIAEL
                     QGITVLSLNSLSDALRPAVLPGEQIRVAIQKEGRERGQGVGYLEDGSMVVVEGGKELV
                     GSDAEVVVSSVIQSSGGRMIFGKPAGPAGS"
     gene            766..1440
                     /gene="ispD"
                     /locus_tag="VM841_02225"
     CDS             766..1440
                     /gene="ispD"
                     /locus_tag="VM841_02225"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HVL89031.1"
                     /translation="MTAWAIVVAAGEGTRLAAGVPKALVPLGGRPMLAWSLEAALGAR
                     SVAGVVVACGDSWLEQGEELVRSIAGKRGRVCGGGPSRHASVRGAIGRVGGDTDRVVI
                     HDAARPLATPTLFERALDGLERAPGAVCAVPVVDTLKRADGDVVAATVDRSVLWRAQT
                     PQAFRLGDLLDAAAGGGEGITDEAMLLERAGRTVVLVPGDERNIKITTPADLELAEAI
                     LRARGR"
     gene            1437..1919
                     /gene="ispF"
                     /locus_tag="VM841_02230"
     CDS             1437..1919
                     /gene="ispF"
                     /locus_tag="VM841_02230"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HVL89032.1"
                     /translation="MSPARVGIGVDAHPFAEGRPLVLGGVVVPHEQGLAGHSDADVLV
                     HAVIDALLGAAGLGDIGSHFPAGDERYRDADSLGLLAETARLVARAGFRVENVDSVVV
                     AERPAIAPLRERMRERMAAALGIEDGRVSVKATTTDGMGFTGRGEGIAATAVALLFGD
                     "
     gene            2453..3766
                     /locus_tag="VM841_02235"
     CDS             2453..3766
                     /locus_tag="VM841_02235"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVL89033.1"
                     /translation="MRDLPVRATLFAFAVAAAAALSIAVAGPDAWPSIAAVIGFGLLF
                     LAADYAPVWFAHVSYSVGFVVAIAAVISIGPAPAAVAATFAAIDRELFSRKDWLPRIM
                     INAGALALSTLVLGSILVSLGGPVGSIGAEDFPGILLPLVLGSTAFFIVNAGLVSVMV
                     SLARRTPLREVWHSDYRRTTTMHFLFAALGVLFAALYTSISWAAVPFVVVPLFAARHA
                     FQTAEKSRETFESTLGSMITALEAKDPYTAGHASRVSQISEMVARAYGFSESKARRVR
                     YAALLHDVGKVTIDTRVLRKPGKLTPEEYDHMKVHSIRGAELIAEIDMLADMVDGVRH
                     HHERVDGAGYPDGLAGEALSDVAKIIMVSDAFDSMTSTRSYRKAMPMEKAFAELRRCE
                     GTQFAPEMIDALERAIARWGWTPAPESYVGELTARPAESVHAAHA"
     gene            complement(3723..4307)
                     /locus_tag="VM841_02240"
     CDS             complement(3723..4307)
                     /locus_tag="VM841_02240"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HVL89034.1"
                     /translation="MFKLMALTIAAAVAAGFLARGRLRRLADVQFRGLPILFVSLGVA
                     LLPLLVEVSDSAARWLTGIANLVVIAFLAVNVRAMRGLIRVGLVVVAAGWMLNAIVIA
                     ANAGMPLSVRAYERSGQTDPITAGQGGFYKIVVADDDTTLRFLGDAIPVRALGQVVSV
                     GDILLSVGIGVAVTGGMRGDATRAPREPIRQAAP"
     gene            4390..>6804
                     /locus_tag="VM841_02245"
     CDS             4390..>6804
                     /locus_tag="VM841_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL89035.1"
                     /translation="MKDVAPFVGRDAELHRIRLALASPGGLLLVSGEAGIGKSRLVRE
                     AAAEAKTEGRAVVWARPDQVSRPGPFALILDLVENLSLLPALAQEASSLADALAEPSD
                     GSHDVVAARRVAARIRGLLAQAGERAIAVFEDLHLADEASHAVIVHLARSAYDDGHLL
                     IATYRPEEVGLAEELERFLGVVTTERLADEIVVDALPQKDSAALAAAILGRDAARHGE
                     RIASVAEGVPFFIEELAEAAASGGSASIPGTISRVVLARAQRVGAVARHVLSAASLMP
                     GAIEPDVLAAAGDLDSGTIARALSEAARAGLVEDRDDRLVFRHALVREAIAAEMVSVE
                     ATDMHRRIASAIEAVYGDDLAPHVRALAHHWYAARDRDRALGYALQAGERALGLAATH
                     EARTAYELAVACTAKPSPDALFGLGEVAVREGRFPDAEDHFRRAAAAWREADDTTGAA
                     RALSRAAFTVAVGSGFSPAFDVVDEALAMLGEGGDPALRASLLAQKGRMVRAGRAAER
                     REALTEALTLSERIGKHEIRSEALDGLAWDAYYGYRLDEAVDAALESARAAIQADQTE
                     AIGRSHNNAAIILSAAGRYAEALDHLAVARERLGVAFGSFGVMFIGVTEALTRWMLGE
                     PNKVGRLLATQNEGWAQSQGAVRLLQAWAAVHAGEQQRARAAVERLRVETGRDGDGDG
                     GVRRPALDSVVCEALVALEQGGRPADEHLDALVQAARASEDMVADERARLLAIAAAGL
                     AAGGRTAESRATLERFDALLEQFPSPYYRARRLEIAGMLCDGDDAAEAFTAAAQAFAS
                     FGNAVDF"
BASE COUNT          953 a         2330 c         2478 g         1043 t
ORIGIN      
        1 gcccatgacg cccccgagcg tgcctgcgac gagatccgcg cccggcagcc gggcgagctg
       61 acgctctgcg agtccgatga gcgtcgcgac cgagcggccg aggatgccgc ccgccacgta
      121 gccggtcgtc gcgccgagca ccgcgcccag cagcagacgc cccggtgttt cgtccgtcac
      181 gatcgactgc gcgatcatgg acccgcgttt gctcgaatgc gtgcgcagcg gattcctgcg
      241 cgggacgatg ctcgtcgggc agttcgtgct cgacgaggtg caggcgatcg ccgactccgc
      301 cgaccaggtc cggcgcaacc gcggcaagcg cggcctcgag atgctttcgg ccctccaccg
      361 tgaaggtctc gcggatttac gcgttgttga caagatgtat ccggagttcg cggaggtgga
      421 cgccaaggtg gtcgcgctcg cgcgtgagcg aggcggctcc atcgtcacca acgacgtcgc
      481 cctcgggcgc atcgccgagc tgcagggcat cacggtcctg tcgctgaact cgctgtcgga
      541 cgcgctccgt cccgcggtgc tgccgggcga acagattcgc gtggcgatcc agaaggaagg
      601 acgcgagcgc gggcaaggcg tgggctacct cgaggacggc tcgatggtcg tggtcgaggg
      661 aggcaaggag ctcgtgggca gcgacgcaga ggtggtcgtc agcagcgtga tccagtcgtc
      721 cgggggccgg atgatcttcg gcaagcccgc cgggccggcc ggatcgtgac ggcctgggcg
      781 atcgtggtcg ccgccggcga ggggaccagg ctcgcggccg gggtgccgaa ggcgctcgtc
      841 ccgctcggcg gccggcccat gctcgcctgg agcctcgaag ccgcgctcgg ggcccggtcg
      901 gtggccgggg tcgtcgtggc ctgcggggac tcgtggctcg agcaaggcga ggagctcgtg
      961 cggtccatcg ccggcaagcg cggccgggtc tgcggcggcg ggccgtcgcg ccacgcgtcc
     1021 gtccggggag cgatcggtcg ggtcggaggc gacaccgacc gggtggtgat ccacgatgcg
     1081 gccaggcccc tggccacgcc caccctgttc gagcgagccc tcgacgggct cgaacgtgcg
     1141 ccgggggcgg tctgcgccgt gcccgtggtc gacacgctca agcgggccga cggggacgtg
     1201 gtggccgcca ccgtggaccg gagcgtgctc tggcgcgccc agacaccgca ggcttttcgc
     1261 ctgggtgacc tgctcgacgc cgcggccgga gggggcgagg ggatcaccga cgaggccatg
     1321 ctgctcgagc gggccggccg cacggtcgtg ctcgtgccgg gcgacgagcg caacatcaaa
     1381 atcacgaccc cggccgatct cgagctggcc gaggcgatcc tgagggcgag gggccgatga
     1441 gcccggcccg tgtcgggatc ggggtggacg cgcacccgtt cgcggaggga cggccgctcg
     1501 tcctcggcgg ggtcgtggtg ccccatgagc agggcctggc cggccattcg gacgccgacg
     1561 tgctcgtcca cgcggtgatc gacgccttgc tcggcgccgc cgggctgggc gacatcggca
     1621 gccactttcc ggcgggcgac gagcggtacc gcgacgcaga cagcctcggc ctgctggccg
     1681 agaccgcccg gctcgtcgcg agggcaggct tccgggtcga gaacgtggat tcggtggtcg
     1741 tggccgagcg gccggccatc gcgccgcttc gggaacggat gcgcgagcgc atggccgctg
     1801 ccctggggat cgaggatgga agggtttcgg tcaaggccac cacgaccgac gggatgggat
     1861 tcaccggccg cggggaaggc atcgcggcga cggcggttgc cctgctcttc ggcgactaat
     1921 ttgtcctgtt tgaccctgtt cgaaccccaa accgcggggt ctccacgtat ttagtcacgt
     1981 ggttcccacg atttttcggg ccccggcacc gccctaccat gcaacacgtc ggcacgagcg
     2041 aaaggcaccg gacatgggac agggaccacg gggcggcaac cgcctccagg cgatggaagt
     2101 ggagcggacc tccgccgtcg cccctgggtt ccttcccgtc tgggacgcgg agacgactgc
     2161 tcctcccgtc tccgatcttt tcgcttcgat cggcgcccgc agcgccgctg gaaaagccga
     2221 atccgaaggt tcaaggggcg gagaagagga aagggggtac ctccaaatga cgcgtttcgt
     2281 gcgtatcgcg acggctctgt ccgcgctggc gactgttttc ctggtctcgg gagcttcgac
     2341 gaagtactaa gccggcgtgc ctcacatgcc gaaagttaag acagcgctct aggaggtctc
     2401 gggcgaaggc tcgggcctcc tagtcgtctg gccgtcggag gatggcatcg gcatgcgaga
     2461 cctacccgtc agagcaacac tgttcgcgtt cgccgtcgcg gcggcggcgg ccttgtcgat
     2521 cgccgtcgca ggcccggacg cgtggccgtc gatcgcggcc gtcatcggct tcggtttgct
     2581 gttcctcgcc gccgactatg cgccggtctg gttcgctcac gtttcctact cggtgggatt
     2641 cgtcgttgcg atcgcggccg tcatctcgat cggtcccgcg cccgcagcgg tcgcagccac
     2701 cttcgccgcc atcgatcgcg agctgttcag ccgaaaggac tggctgcctc ggatcatgat
     2761 caacgccggc gcgctggcct tgtcgaccct ggtgctcggc tccatcttgg tctcgctcgg
     2821 cggtcccgtc ggatcgatcg gggcggagga cttcccgggc atcctgctgc cgctggtgct
     2881 cggctcgacc gcgttcttca tcgtcaacgc ggggctggtc tcggtgatgg tgtcgctcgc
     2941 gcgccgaacg ccgctgcgcg aggtctggca ctcggactac cggcggacca cgacgatgca
     3001 cttcctgttc gcggcgctcg gcgtgctgtt cgctgcgctg tacaccagca tctcctgggc
     3061 ggcggttccg ttcgtcgtcg tcccgctgtt cgccgcacgg cacgcgttcc agacggccga
     3121 gaagtcgcgc gagaccttcg agtcgacctt gggcagcatg atcaccgccc tcgaggcgaa
     3181 agacccgtat acggccggtc acgcgagccg ggtgtcgcag atatcggaga tggtcgcgcg
     3241 cgcgtacggc ttcagcgagt ccaaggcacg ccgcgtccgg tacgccgcgc tgctgcacga
     3301 cgtcggcaag gtgaccatcg acacgcgcgt gctgcgcaag cccggcaagc tcacgccgga
     3361 ggagtacgat catatgaagg tccactccat ccggggcgcc gagctgatcg cggagatcga
     3421 tatgctcgcg gacatggtcg acggcgttcg tcatcaccac gagcgtgtgg acggcgccgg
     3481 ctatcccgac gggctcgccg gcgaggcgct gtcggatgtc gccaagatca tcatggtttc
     3541 ggatgcgttc gactcgatga cctcgacccg ctcgtaccgc aaggcgatgc cgatggagaa
     3601 ggccttcgcc gagcttcgcc gctgcgaagg gacgcaattc gcgcccgaga tgatcgacgc
     3661 gctcgagcgt gcgatcgctc gatggggctg gacgcccgcg cccgaatcct acgtgggcga
     3721 gctcacggcg cggcctgccg aatcggttca cgcggcgcac gcgtagcgtc gccccgcatc
     3781 ccgcccgtca ccgccacgcc gatgccgaca ctcagcagga tgtccccgac ggacacgacc
     3841 tggccgagcg cccggaccgg gatcgcatcg ccgaggaacc gcagcgtcgt gtcgtcgtcc
     3901 gcgaccacga tcttgtagaa cccgccctgc cccgcggtga tcggatccgt ctgtccggat
     3961 cgctcgtagg cccggaccga taagggcatg cccgcgttcg cggcgatgac gatcgcgttg
     4021 agcatccatc cggcggcgac gaccacgagc ccgacgcgga tcagaccccg catcgcccgg
     4081 acgttcacgg ccaggaacgc gatcaccacg aggttggcga tgccggtgag ccagcgtgcc
     4141 gccgagtccg agacctcgac cagcaacggc agcagcgcga ccccgagcga gacgaacagg
     4201 atcggcaggc cgcggaactg cacgtcggcg agccgccgga gccgtccgcg ggcgaggaag
     4261 ccggccgcga ccgcggcggc gatcgtgagg gccatgagct tgaacatttt gacgatcctc
     4321 ggtcgcggac ggcgggccag agccgatggt agacgatgat ccgctaggct ccacggcggg
     4381 aggggagccc tgaaggacgt cgcacctttc gtcggacgcg atgcggaact ccaccgcatc
     4441 cggctggcgc tcgcctcgcc cgggggactt ttgctcgtat cgggtgaagc cggaatcggc
     4501 aagtcgcgac tggtgcgcga agccgccgcc gaagccaaga ccgagggccg ggccgtcgtg
     4561 tgggcccgtc cggatcaggt cagccgtccc ggcccgttcg cgctgatcct cgacctcgtc
     4621 gagaatttgt cgctgctgcc cgcgctcgct caggaggcgt cgtcactcgc ggacgccctg
     4681 gccgagccgt cggacggctc gcacgacgtc gtcgcggccc gccgtgtcgc cgcgcgcatt
     4741 cgcggccttc tggcccaggc cggcgagcga gcgatcgcgg tcttcgaaga ccttcatctg
     4801 gccgacgagg cttcgcatgc cgtcatcgtg catctggctc gctcggccta cgacgacggg
     4861 cacctgctca tcgcgaccta ccggcccgag gaggtcggac tggccgagga gctcgagcgc
     4921 ttcctcggcg tggtcaccac ggagcggctg gccgacgaga tcgtcgtcga tgcccttccc
     4981 cagaaggact cggccgcgct cgcggccgcg atcctcggcc gggacgcggc acggcacggc
     5041 gagcggatcg cgtcggtcgc agaaggcgtc ccgttcttca tcgaggagct cgcggaagcg
     5101 gccgcgagcg gtggcagcgc gagcatcccg ggcacgatct cgcgggtggt tctcgcgcgc
     5161 gcgcagcgtg tcggggcggt cgcacggcat gtgctctcgg cggcgtcgct catgccgggc
     5221 gcgatcgagc ccgacgtgct cgccgcggcc ggcgatctcg attccggaac catcgcgcgc
     5281 gcgctgtcgg aggccgcccg tgccggcctc gtcgaagacc gcgacgaccg gctggtgttt
     5341 cgtcacgcgc tcgtgcgcga ggccatcgcc gccgagatgg tcagcgtcga ggcgaccgat
     5401 atgcatcggc ggatcgcgtc ggccatcgaa gcggtgtacg gggacgatct cgccccgcac
     5461 gtgcgcgcgc tggcgcatca ttggtacgcg gctcgtgacc gcgatcgtgc gctcggctac
     5521 gcgttgcagg cgggggagcg cgcgctcggc ctcgccgcga cccacgaggc ccgcaccgcc
     5581 tacgagctcg cggtcgcatg cacggccaag ccctcgcccg acgcgctgtt cggactcggg
     5641 gaggtcgccg tgcgcgaagg ccggttcccc gacgccgagg atcacttccg ccgtgcggcc
     5701 gccgcgtggc gcgaggccga cgacacgacc ggcgcggcgc gcgctctgtc gcgcgccgcg
     5761 tttacggttg cggtcggatc gggcttctcg ccggcgttcg acgtggtgga cgaagcgctc
     5821 gcgatgctcg gtgagggagg tgatcccgcc ctgcgcgcgt ctttgctggc acagaagggc
     5881 cgcatggttc gcgccggccg cgctgcggag cgccgcgagg cgctcaccga agccctcacc
     5941 ttgtctgagc gcatcggcaa gcacgaaatc cgctccgagg cgctcgacgg gctcgcgtgg
     6001 gacgcgtact acggctaccg attggacgag gccgtcgatg ccgctctgga atcggcgcgt
     6061 gcggcgatcc aggccgatca gaccgaggcg atcggccggt cgcacaacaa cgccgcgatc
     6121 atcttgtccg ccgcaggccg ctacgcggaa gcgctcgatc atctggccgt cgcgcgcgag
     6181 cggctcggag tggccttcgg gtcgttcggg gtgatgttca tcggggtgac ggaggcgctg
     6241 acccggtgga tgctcggcga gcccaacaag gtcggccgtc tgctggccac gcagaacgaa
     6301 gggtgggccc agtcgcaggg cgcggtcagg ctgctgcagg cctgggccgc ggtgcatgcc
     6361 ggcgagcagc agcgggctcg cgccgccgtc gagcgccttc gcgtcgagac cggccgggat
     6421 ggagacggcg acggcggcgt ccggcgcccg gcgctcgatt cggtggtctg tgaggcgctc
     6481 gttgcgctcg agcagggcgg gcggccggcc gacgagcacc tggacgcgct cgttcaggct
     6541 gcccgcgcca gcgaggatat ggtcgccgac gagcgggcgc gtctgctggc gatcgccgct
     6601 gccggtctgg ccgcgggcgg ccgcaccgcc gagtcgcggg cgacgctgga gcgattcgac
     6661 gcgctgttgg agcagtttcc ctcgccgtac taccgagccc gccggctcga gatcgcggga
     6721 atgctctgcg acggcgacga cgcggccgag gcgttcaccg cggcggccca ggccttcgcg
     6781 tcgttcggaa acgcggtgga cttc
//
LOCUS       DATLFO010000065         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_51083, whole genome shotgun sequence.
ACCESSION   DATLFO010000065 DATLFO010000000
VERSION     DATLFO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51083"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VM841_02250"
     CDS             complement(<1..1671)
                     /locus_tag="VM841_02250"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HVL89036.1"
                     /translation="MTHRLRLLLALALVSLPLILVASPAQAKSFSFPRVQVDIDVQPD
                     GSFRVREERTFDFDGSFSRGFLAIPAGRYEITDVGVAEDGTVYEAGPRYPEIPGRFSF
                     ESSPRYRIDWFYLAADERRTFVITYTVRGAVVAHNDVAEFYWQFIGDEWDQGVDEVVA
                     TVRLPAGATREQVRVWGHGPLEGDVRIQDPQTLVWEVSNLRPATFVEGRIAFPPSLVP
                     GAPKSNEARLPAILREEQEWADEANRARAVARFWNIASPVAALAALGVFVFLFFRYGK
                     EPDVPAPGRYLREPPADYSPAILGYLMRFGKTRPVDMAAVLMDLARRGHLQIDEVREE
                     EGLIFKRPEYHYNITRTGKSRDTLTPYEAEVMKMIERAGGGSRVVREDALKSWAKANP
                     SSAQSHFQSFKEKVKADAQSRGLLEPRAGVMVWNGVAAAIAIVVTMVALGRQIGEPAS
                     AVVPGIVFLIAGMFLVRESRLVGLLMIGSSGIAVAALVAGRAWIYAPGPLAGLLIVIA
                     VAAMTPLLQRRTGRGATDFRGWKSFRRFLKDRSRMEEKRPGSVVLWEKY"
     gene            complement(1668..>2137)
                     /locus_tag="VM841_02255"
     CDS             complement(1668..>2137)
                     /locus_tag="VM841_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HVL89037.1"
                     /translation="ELVEPLRNLFKDEVRAIGEELGLHEEIVWRQPFPGPGLAIRVIG
                     AVDREKLEILRNADAIVLEEIRREDMSRSIWQSFAILPDIRTVGVMGDGRTYAHPIIL
                     RAVTSEDAMTADWARLPYEVLERISSRIVAEVPGVNRVAYDITSKPPGTIEWE"
BASE COUNT          368 a          766 c          687 g          316 t
ORIGIN      
        1 atacttctcc cacagcacca ccgagcccgg ccgcttctcc tccatgcgcg accggtcctt
       61 cagaaagcgc cggaacgact tccagccgcg gaagtcggtc gcgccgcggc cggtgcggcg
      121 ttgcagcagc ggcgtcatcg cggcaaccgc gatcacgatc aacagcccgg ccagcggacc
      181 gggcgcgtag atccacgcgc ggccggcgac cagcgcggcg acggcaatac cgctcgatcc
      241 gatcatcagc agacccacca gccggctttc gcgcacgagg aacatgccgg cgatcaggaa
      301 cacgatgccg ggcacgacgg cggaggcagg ctcgccgatc tgacggccca gcgcgaccat
      361 cgtgacaacg atcgcgatgg ccgccgcgac gccgttccac accatcacgc cggcgcgcgg
      421 ctcgagcagc ccgcgcgact gcgcatcggc cttgaccttc tccttgaacg actggaagtg
      481 agactgcgcg gatgacgggt tggccttcgc ccacgacttg agcgcgtcct cgcgcacgac
      541 gcggctgccg ccgcccgcgc gctcgatcat cttcatgacc tcggcctcgt agggcgtgag
      601 cgtgtcgcgg ctcttgccgg tgcgcgtgat gttgtagtga tactcgggcc gcttgaagat
      661 cagaccttcc tcttcgcgca cctcgtcgat ctgcagatgg ccgcggcggg ccagatccat
      721 caggaccgcg gccatgtcca ccggcctggt ctttccgaag cgcatcagat agcccaggat
      781 cgccggcgag tagtcggcgg gcggctcgcg cagataccgg ccgggcgcgg gcacgtcggg
      841 ctctttgccg tagcggaaga acaggaacac aaacacaccc agcgcggcga gcgccgccac
      901 cggcgatgcg atgttccaaa agcgcgcgac cgcgcgcgcg cggttcgcct cgtcggccca
      961 ctcttgctct tcccgcagga tcgcgggaag acgcgcctcg ttcgacttcg gtgcgcccgg
     1021 caccagcgac ggcgggaacg cgatccgtcc ttcgacgaag gtcgccggcc gcaggttcga
     1081 cacctcccac accagcgtct gcggatcctg gatccgcacg tccccctcca gcggtccgtg
     1141 accccacacg cgcacctgct cgcgcgtggc gccggcgggc agacgcacgg tcgcgacgac
     1201 ctcgtccacg ccttggtccc actcgtcgcc gatgaactgc cagtagaact cggcgacgtc
     1261 gttgtgcgcg acgaccgcgc cgcgaaccgt gtaggtgatc acgaacgtcc gccgctcgtc
     1321 ggcggccaga tagaaccagt cgatgcgata tcgcgggctg ctttcgaacg agaaccggcc
     1381 gggaatctcg ggatatcgcg gtcccgcctc gtagaccgtc ccatcctccg ccacaccgac
     1441 gtcggtgatc tcgtagcgac ccgcgggaat cgccaggaat ccgcggctga acgagccgtc
     1501 gaagtcgaag gtgcgctcct cgcgcacgcg gaacgagccg tcgggctgca catcgatgtc
     1561 gacctgaacc cgcgggaacg aaaacgactt cgcctgcgca ggcgaggcaa ccagaatcag
     1621 cggaagcgaa acgagggcga gcgcgagcag caaccgaagc cggtgcgtca ttcccactcg
     1681 atagtgcccg gcggcttcga ggtgatgtcg taggcgacgc ggttcacgcc cggcacttcg
     1741 gcgacgatgc gcgacgagat acgctccaag acttcgtagg gcagccgcgc ccagtcggcg
     1801 gtcatcgcgt cctcgctggt gacggcgcga aggatgatcg gatgcgcgta cgtccggccg
     1861 tcgcccatca ccccgacggt gcggatgtcg ggaaggatcg cgaacgactg ccagatcgag
     1921 cgcgacatgt cctcgcggcg gatctcttcc agcacgatcg catccgcgtt gcgcagtatc
     1981 tcgagcttct cgcgatcgac cgcgccgatc acgcggatcg cgaggcccgg ccccgggaag
     2041 ggctggcgcc acacgatctc ttcgtgcagg cccagctctt cgccgatcgc gcgcacctcg
     2101 tccttgaaca ggttccgcag cggctcgacc agttcga
//
LOCUS       DATLFO010000066         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_51181, whole genome shotgun sequence.
ACCESSION   DATLFO010000066 DATLFO010000000
VERSION     DATLFO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51181"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VM841_02260"
     CDS             complement(<1..676)
                     /locus_tag="VM841_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL89038.1"
                     /translation="MSEKVTTEDRGAVRILRINRPEVRNCVDGETAVGLGRAIEAFAT
                     DESVRVLVVTGEGDLSFCAGADLKNAPTLMDHEYVDKAAPMGFARLDPGKPTIAAING
                     FCFAGGFELAAWCDIRIAAANAEFGCLSRRWGVPYTDGGTQRFSRIMGMGNALYLLVT
                     GARIDARRAYEMSFVQEVVPHGQALSRAIALAEQIAAYPNQPGIRADRAAALQGFTMD
                     LDEALLL"
     gene            773..1597
                     /locus_tag="VM841_02265"
     CDS             773..1597
                     /locus_tag="VM841_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4344 domain-containing metallopeptidase"
                     /protein_id="HVL89039.1"
                     /translation="MKTFRRRRAAAIALAAAALLGACGGEDTPAGGGSASPATETVTD
                     AGDLTVEFATPKAAAYGDYPELLRDFVNESVSAVNELLALPEDIPMVFAEIGEENAYY
                     DPEQRTITFGYELVAAYVELAPAVRETEDEQDQFIYANVENTMLHELGHALIDILELP
                     VLGKEEDAADGLATLIQIEAFEEGTAYAIDVADGWAAVAESPGSLDESAFADEHSLNA
                     QRFYATACWVAGSSDDAMEQVEAAEVLPPERLERCPEEYELLAESWSAVLDPYLKS"
     gene            complement(1641..2468)
                     /locus_tag="VM841_02270"
     CDS             complement(1641..2468)
                     /locus_tag="VM841_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVL89040.1"
                     /translation="MPRSIWSGAVNFGLVSIPVKLYSAVSQKDVSFHQIDRRSGSRIK
                     YKRVSTKSGREVPYEEIAKGYEVSKDEYVVFDQEELEELDPERNHAVDIEDFVELDEI
                     DPIYFESTYYLAPGQGGNKAYSLLRKALEDAGRIGIGKVVLRTKEYLCAIRPLGKALA
                     LSTMLYPDEIVQPGTIKDMPDRAATISDRELKAARQLIDSLATEFDPKKYKDTYRERV
                     LEAIDKKAKGETLEIDAPSEDAPKVLDLMEALRASVEAASSGKQKKKAGSSHKRKSA"
     gene            2577..>2869
                     /locus_tag="VM841_02275"
     CDS             2577..>2869
                     /locus_tag="VM841_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89041.1"
                     /translation="MRCYVDYTCGHSYRLWSWLRGLNTGLDWRTFSLKEVNREPDEPR
                     AFENLGSISVLALALSHAARNAGFLRYHGAAFEAFHAGRVAEEDLLVLAAQAGV"
BASE COUNT          445 a          990 c          938 g          496 t
ORIGIN      
        1 ccagcagcag cgcctcgtcc agatccatgg tgaatccttg aagcgcggcg gcgcgatcgg
       61 cgcggatccc tggctgattc ggatacgcgg cgatctgctc ggccagcgcg atcgcgcgcg
      121 acagcgcctg gccgtgcggc acgacttctt gcacgaaact catctcgtag gcgcgtcgcg
      181 catcgattcg cgccccggtt acgagcaggt acagggcgtt gcccatgccc atgatgcgcg
      241 agaagcgttg cgttcccccg tcggtgtagg gcacgcccca gcggcgtgac aggcatccga
      301 actccgcgtt ggccgcggcg atgcggatgt cgcaccacgc ggcgagctcg aagccgccgg
      361 cgaagcagaa gccgttgatc gccgcgatgg tcggcttgcc gggatccagc cgcgcgaagc
      421 ccatcggcgc ggccttgtcc acgtactcgt ggtccatgag cgtcggcgcg ttcttcaggt
      481 ccgcgccggc acagaaagac aggtcccctt ccccggtgac gaccagaacg cggacgctct
      541 cgtcggtcgc gaacgcctcg atcgcgcggc cgagccccac cgccgtctcg ccgtccacgc
      601 agttgcggac ctcgggccgg ttgatccgaa ggattcgcac ggctcctcga tcttcggtcg
      661 ttaccttctc gctcaacgca tgcctcctcg ctcggtgtcg ttcatgctat ccggcggccc
      721 tgccgcatcg cggccgcgag cccgcagccg ggcccctgct accctgcgcg ccatgaagac
      781 cttccgaaga cgacgcgcgg cggcgatcgc gctggccgcg gccgcactgc tcggagcctg
      841 cggcggcgag gacacacccg ccggcggcgg cagcgcaagc ccggcgaccg aaaccgtcac
      901 cgacgcgggc gatctcaccg tcgagttcgc gacgccgaag gcggctgcct acggcgacta
      961 tcccgagctg cttcgcgact tcgtcaacga gtcggtctcc gcggtcaacg agctgctcgc
     1021 gttgccggag gacatcccga tggtctttgc cgagatcggc gaggagaacg cctactacga
     1081 ccccgagcaa cgcacgatca cgttcggcta cgagctcgtc gccgcttacg tcgagctcgc
     1141 gcccgccgtg cgcgaaaccg aagacgagca ggatcagttc atctacgcca atgtcgagaa
     1201 cacgatgctg cacgagctcg gtcacgcgct catcgacatc ctcgagctgc ccgtgctcgg
     1261 caaggaggag gatgcggccg acgggctcgc gacgctgatc caaatcgaag cgttcgaaga
     1321 gggcaccgca tacgcgatcg acgtcgccga cggctgggcc gcggtcgcgg agtcgcccgg
     1381 ctcgctcgac gagtcggcct tcgccgacga gcacagcctc aacgcgcagc gcttttacgc
     1441 gaccgcatgc tgggttgcgg gcagcagcga cgacgccatg gagcaggtcg aggccgccga
     1501 ggtgctgccg cccgagcggc tggagcgctg ccccgaggaa tacgagctac tcgccgagag
     1561 ctggtcggcg gtgctggacc cctacctgaa gtcgtagcgc ggcttcgccg ggccgtccgc
     1621 gcgacagggc cgcgcaacca tcaggcggat ttgcgcttgt gcgagctgcc cgccttcttc
     1681 ttctgcttgc cggatgaggc ggcctcgacg gatgcgcgca acgcctccat gaggtcgagc
     1741 accttcggcg catcctcgga cggcgcatcg atctcgagcg tctcgccctt ggccttcttg
     1801 tcgatcgcct ccaggacgcg ctcgcgatag gtgtccttgt acttcttcgg atcgaactcg
     1861 gtggcaagtg agtcgatcag ctggcgcgca gccttgagct cgcggtcgga gatcgtcgcc
     1921 gcgcggtcgg gcatgtcctt gatcgtgccc ggctgtacga tctcgtcggg atacagcatc
     1981 gtcgacagcg cgagcgcctt gcccaacggc cggatcgcgc acaggtactc cttggttcgc
     2041 agcacgacct tgccgatccc gatgcggccg gcgtcctcga gcgccttgcg caggagcgag
     2101 taggccttgt tgccgccctg gccgggcgcg agatagtagg tgctctcgaa gtagatcggg
     2161 tcgatctcgt ccagctcgac gaagtcctcg atgtctaccg cgtggttccg ttcggggtcg
     2221 agctcttcga gctcttcctg atcgaacacg acgtactcgt ccttggagac ctcgtagcct
     2281 ttggcgatct cctcgtaggg gacctcgcgg ccggacttcg tgctgacgcg cttgtacttg
     2341 atgcgcgagc cgctgcgacg gtcgatctga tggaacgaca cgtccttttg cgatacggcg
     2401 ctgtagagct tgacggggat gctgacgagc ccgaagttaa cggctccgct ccagatcgaa
     2461 cgcggcatcg aagcaacacc tcctgcccgg tcgtataccc ctcgtatacc cggaatcgcg
     2521 gccgggcacg cagggggaga cggcgcaaag cggacgcgcc gcgctacagt gccgccatgc
     2581 gctgctacgt ggactacacc tgcggtcatt cctaccgcct ctggtcttgg ttgcgcggcc
     2641 tgaacaccgg cctcgattgg cgcaccttct cgctcaaaga ggtcaaccgc gagccggacg
     2701 agccgcgcgc cttcgagaat ctcggctcga tctcggtgct ggcgctcgcg ctgtcgcacg
     2761 cggcgcgcaa cgccggcttt cttcgatatc acggcgcggc gttcgaggcg ttccatgccg
     2821 gccgcgtcgc cgaggaagac ctgctcgttc tggcggcgca agcgggcgt
//
LOCUS       DATLFO010000067        10531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_130567, whole genome shotgun sequence.
ACCESSION   DATLFO010000067 DATLFO010000000
VERSION     DATLFO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130567"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1817)
                     /locus_tag="VM841_02280"
     CDS             complement(105..1817)
                     /locus_tag="VM841_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89042.1"
                     /translation="MRDRIRLIVTTLTILLVVAALGSTAALASHAGNGTQRGGFEIDG
                     DYLYPHAPPIAGATRDWQTIGEVLRVNDAFAPSSDSIFTGGSKEQDPDGWVFSSGSVP
                     GKDDLTRLYFAGDVVDPTKAFLFLAFERLDVSGNGDVHVNFELNQSSATVTNSKGTVI
                     PRRSSGDLLVVYDYDGGTASTSIDIEIRLWVGDALSGQWGPDIGGADAVGDVNGAGAV
                     TRPAESPFGGGTVDAKRFGEVAINLLAIYPNMFTDCVSFTNFWAKTRASGESFDSALK
                     DRTASTRVEFSSCPDVPSIIEVEKKADNESVGAGNPIGYTITVTNTGDVVALGVRLTD
                     DLPAGEGLVWSVDGGSAASDCAISARMLECALGDMPAGASRTVHVASPTTAATCGRLS
                     NKATATAVNGGSDTSPVATVDVMCAAIAIVKTVTPVSGAPGDPVTYSYRVTNTGSTTL
                     LDLSVDDDKLGHIGDIGRLDAGQSVTLTKASTLGSTGVTNVGCVEGTDEFGSRARACD
                     DATVVVVLGSRFTLPATGIDAAPASTIAAVLVFAGLTFLAAARRREVKGAPAAAPRGR
                     ANSA"
     gene            complement(1850..2071)
                     /locus_tag="VM841_02285"
     CDS             complement(1850..2071)
                     /locus_tag="VM841_02285"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HVL89043.1"
                     /translation="MALLVAATVFALQNLKGVDVTFLSFHGQLPLAVLLLVVAVLGSL
                     AVFAFGAARVLQLRLHTRRLGRELSKRGD"
     gene            2572..2662
                     /locus_tag="VM841_02290"
     tRNA            2572..2662
                     /locus_tag="VM841_02290"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2606..2608,aa:Sec,seq:tca)
     gene            complement(2894..4057)
                     /locus_tag="VM841_02295"
     CDS             complement(2894..4057)
                     /locus_tag="VM841_02295"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HVL89044.1"
                     /translation="MPSSESGGVPRRDFLRGAVALGASWALLPRLAGAEPGSGGQWLA
                     GDLHCHTVYSGDVWAPGDDNTSETEAYTFGWTAAEQILIAESRGLDYLAITDHDDLRW
                     LGDGTTGGGHALTLVPGYEHSLRRGHAGCLGVFQRFPIDTQTEAGALALRDAVRDAGG
                     LFILNHPFYKNHPPTGADGIPGWGYSPATVVPDSVEVWNIAWPYRRPAFQGISTSENY
                     LSLPWWDREFLGRGHRVPATGGSDNHWRSTAAAQGVGQPTTWVYSESNTWQGILDAIR
                     AGRTFVSAQPPAYGGTQIYLSAEKGSEQRIVGDVVPAAWGEVTVAARIVGAPAGFVRF
                     VVDGVQGSLQPIAGSDVTVTRTVDASTHSRVRAEVVADDGWWMIALTSPIYFA"
     gene            complement(4107..4610)
                     /locus_tag="VM841_02300"
     CDS             complement(4107..4610)
                     /locus_tag="VM841_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVL89045.1"
                     /translation="MDKRRAHRADRTAVRLARAYGILSLRISLGLVFVWFGALKVFGV
                     SPVAGLVADMIPVVPDRTAVVLMGAIEIGVGIGLLTGWLIRVTLGVFFAQMLGTFLVF
                     VARPSLSFAGGNPLELTVLGEFILKNLVLITAGIVVAAATIPRPQPDEGLGEMLTKKA
                     ESERAAS"
     gene            complement(4736..6196)
                     /gene="ppcA"
                     /locus_tag="VM841_02305"
     CDS             complement(4736..6196)
                     /gene="ppcA"
                     /locus_tag="VM841_02305"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVL89046.1"
                     /translation="MTGVPRCMSTQHPDNVDPPFFADAPALSGEDEIEEAYYAYSHLG
                     CDEQMWDAEGKEVDGFVVKKLLGKYPSFFDANRLGRDIRLTLRVPNPAVETTEAKILL
                     ETLESIARSSDATRPFYDGATPPIFEVILPMTTSARELNRIYRYYRDIVVGKQTQRLG
                     DDDITVAEWVGSFEPEHIEVIPLYEDLDNMLAADRITEEYLRDKDVTTQRVFLARSDP
                     AMNYGMLAAILMAKIALRRLHELEERSGVKIAPILGVGSAPFRGGLTPDAVDQIMANH
                     PSVHTFTVQSAFKYDHPPGMVRDAIARIRAHTPSMPPAVDEERCIEIINRTAQAYRKH
                     VVELAPVVQRLARFVPARRTRKLHVGLFGYARDVAGVSLPRAISFTAALYSVGLPPEV
                     LGIEALTKDDHAFLSEAAPRMMFNLRRAGRYLRADSDLVPPEVARAARELYLCDEPDE
                     EHHALTAEIADGARGTDTADLAPAMLRAARQRRFLG"
     gene            6333..7499
                     /locus_tag="VM841_02310"
     CDS             6333..7499
                     /locus_tag="VM841_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89047.1"
                     /translation="MRRVSLVLMSLTSLAASLLPSSATATPGSHPGFLFNAQDNRLDV
                     YDLSAAHPALARSTPIHAHDHDPDAGPGEDPVGRGNDVNGQICRVVQADGSVRFVMGE
                     DSDQESLPAGIAQGWGLFEPSDGAEGPWTMTGKIVAPFRVHDNDHQPETTGCAVSADG
                     SMLFLVDLGVGAFDVPGVGSLFLYRRDAGGNFSASSPVCVLADDLTTAGYIAMHPDGS
                     VLVPEGGRFEGGVVSRFRPPFPAAGDAAGCAAYRAAHGVADRESFLGPFAMLPFDPVS
                     FVPISIARRGSGWLIGNVFPGQVAEYDGSGSFVRVITAGQGPGVAGIAVDGHGNVYIA
                     NLGLVPCETLLCPASGAGTLWKVAFDPLTDAPLPPVLVMAGLTYPEGIAVFPSL"
     gene            complement(7508..8770)
                     /locus_tag="VM841_02315"
     CDS             complement(7508..8770)
                     /locus_tag="VM841_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVL89048.1"
                     /translation="MPGTNEELSEPGATLLESLPAPPDDGFNPFDPAFLADPYPEYAR
                     LRDTYPVYTTPLGFTVLFRHDDVVDFLRDPALSVEERHQRPTPVTQMIEALVGERAEI
                     GNKSMLNRDAPDHTRLRRLVSQAFTPKTIESLTPRIRSFVDRALEPLSSGDEIDLIST
                     LAFPVPFEVICEMLGMPPTDRDDVRAWSHLMVRSLEPLVDPELIVAIADAGDQMRALL
                     ADVIAWKRQYPAPDMLSAMIAAEEQGDVLTDEELLEQLMLLFIAGHETTVNLIGNGVR
                     ALLRNPESIPRFANDPVNAVEELLRFDSPVQMSRRITLREVEVRGQTIEQGQFVVLVL
                     ASANRDAAFWGGDAERLDLARAMAPQNLSFGGGHHYCLGAALARLEGRIAIGSLLERF
                     PNTELAGEPQVNGRINLRGYERLPVRLS"
     gene            9056..9676
                     /locus_tag="VM841_02320"
     CDS             9056..9676
                     /locus_tag="VM841_02320"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVL89049.1"
                     /translation="MTPLKKSLPTTSYAVLGILSIHPMSGYELAQAAERSIANFWPIS
                     RSQVYSELARLEDLGLIAGADVAQERVPDKRVFEITPAGEDAFTEWVSAPGYEPTRMR
                     LGFCLKTFFGHHMPRETMVENLERFKKENEEQIAYLQRVVNMLGVLPEAAFTRATAEL
                     GLRIARTSAQWAEELLANVPEIRHSSGEEHEEVHRIARELFRKVGR"
     gene            complement(9770..9928)
                     /locus_tag="VM841_02325"
     CDS             complement(9770..9928)
                     /locus_tag="VM841_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89050.1"
                     /translation="MSDEREARCVHCGDSIECCEFCDRVDCPEASCFGCIRIRLGTMT
                     SEPHEHGG"
     gene            complement(9925..10368)
                     /locus_tag="VM841_02330"
     CDS             complement(9925..10368)
                     /locus_tag="VM841_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL89051.1"
                     /translation="MFDYGAGKLDWLAAGNPTEGTNPAKARAGAVARHDVPTCRVDEP
                     LGDVAARVAAAGFDACVVVNDAGVVFGLLRSAELARDAGAIVGDVMRPGPSTFRPHVM
                     AGEMARYMVKHDLTSSPVTRSDGTLVGLLYRDDVTTYLHEHGDDE"
BASE COUNT         1737 a         3566 c         3552 g         1676 t
ORIGIN      
        1 agtccttgcc acgtcctcga gcctcggtgt atcggtcgtt ccgtgaatgg aagtcggctt
       61 catcgtgacg atcgctcgtc acggccgtgt caccgatcga tgagctacgc ggagttcgcc
      121 cggccacgcg gcgccgccgc cggggctccc ttcacctcgc gacgccgggc cgcggccaaa
      181 aaagtaaggc cggcgaagac caaaaccgcg gcgatcgtcg aagccggagc ggcgtcgata
      241 ccggtcgccg gcagggtaaa gcgggagccc aagaccacga cgacggttgc gtcgtcgcag
      301 gcgcgcgcgc ggctgccgaa ctcatcggtt ccttcgacac agccgacgtt ggtgacaccc
      361 gtcgatccca gggtggatgc tttcgtcagc gtgacgctct ggccggcatc cagccggccg
      421 atgtcgccga tatgaccgag cttgtcgtcg tcgaccgaca ggtccagcag cgtcgtgctt
      481 ccggtgttgg tgacgcggta ggagtaggtg accggatcgc cgggcgcacc cgagacgggg
      541 gtgacggtct tgacgatcgc aatcgcggcg cacatcacgt ccaccgtcgc gacggggctg
      601 gtgtccgagc ccccgttgac cgccgtcgcc gtcgccttgt tgctcagccg gccgcacgtc
      661 gcggccgtcg tgggagacgc cacgtgcacg gtccggctcg caccggccgg catatcgcca
      721 agggcgcatt cgagcatccg cgccgagatc gcacaatccg aagcagcgct tccgccgtcg
      781 acgctccaaa ccagcccctc gccggccggc aggtcgtcgg tcaagcgcac gcctaacgcg
      841 acgacatcgc cggtattcgt gacggtgatc gtgtatccga tcgggttgcc tgccccgacc
      901 gattcgttgt cggccttctt ttcgacctcg atgatgctcg gcacatcggg gcaggaagag
      961 aactcgacac gcgtcgaggc cgtccggtcc ttcagcgcgg agtcgaacga ctcgccggat
     1021 gctcgggtct tggcccagaa gttcgtgaag ctgacgcagt ctgtaaacat attcggatag
     1081 atggcgagca ggttgatcgc aacctcgccg aatcgcttgg cgtccaccgt gccgccgccg
     1141 aatggtgact ctgcgggtct ggtgacggcg ccggcgccgt tgacgtcacc gaccgcgtcc
     1201 gcccctccga tatcgggccc ccactggccc gacaacgcgt cgcccaccca aagccggatc
     1261 tcgatgtcga tcgaggtcga tgcggtgccg ccgtcgtagt cgtagacgac cagcaagtcg
     1321 ccggacgatc ggcgaggaat cacggtccct ttgctgttgg tcaccgtcgc cgaggactga
     1381 ttcagctcga agttcacgtg cacgtcgccg ttgccggaga cgtccagacg ctcgaaagcc
     1441 aggaacaaga acgccttcgt ggggtcaacg acgtcgccgg cgaagtacaa gcgtgtcagg
     1501 tcatctttcc ccgggacgct cccgctcgag aacacccagc cgtcgggatc ctgctccttg
     1561 ctgccgccgg tgaagatcga atccgaagac ggagcgaacg cgtcgttgac tcgcaggacc
     1621 tcgccgatgg tttgccagtc cctcgtcgcc ccggcgatcg gcggcgcatg gggataaaga
     1681 tagtcgccgt cgatctcgaa accgcctcgt tgagtaccgt tgccggcgtg ggacgccaga
     1741 gcggcggttg aacccagggc ggcgacgacc agcagaatcg tcaaggtcgt gacgatcagt
     1801 cgaatacgat cacgcatgat tttcctctcg tgatgcttgg cagcggtgtt cagtcgccgc
     1861 gcttcgaaag ctctcggccc aagcgccgcg tgtgcagccg gagctggaga acacgcgcgg
     1921 ctccgaaggc gaagacggcc agggaaccga gcacggccac caccaatagc aggaccgcca
     1981 acggcagctg gccgtggaag ctcaagaacg tgacgtccac gcctttcagg ttctgcagcg
     2041 cgaagacggt ggccgcgacg agaagcgcca agccgaagct gatccatccc cacgcacggc
     2101 cagtacgcgt gctggtcgaa ttgatcgccc ccgctgctcg tgctcgccgc ccatcatgac
     2161 catgggccga aagatccgag cgcgcagacc acgtgttttt cacgtccgca ttgtgcgaag
     2221 acaccgtcac gccggtgtca ccgcggccgc gttcagcccg ttcccatgct gccgatcgct
     2281 tgcaccaaga cacgctgccg aaatcgggta ttgatgacgg ccgggcggtg acgcgcgggt
     2341 gacgaccgtg atccaggatg ccggttcgac ggcccgagcg gctgaagctt cagatcacag
     2401 gcgcgggaga tcgcgccgga tcgatctacg accaacgcct ggtcgtgtag tgccgaatat
     2461 gccgaacgat cgggctgacg agtcgacgct cggcttcgag ccggcgtgaa gcccgaggct
     2521 cacggcgggc cccagaagcg aaccgccccg gccgcgacta cgattgaccg cggaggcgaa
     2581 cgggggctgg tgtccctccc ggtcttcaaa accggtgaag cgtctaaccg cgcttggtgg
     2641 gttcgattcc ttccgcctcc gcttagcacg agcgaacaag cagctgggcg acgaacacgc
     2701 aggtcgtaag cgcttcatca tccgtcaccc gatccgttcc gtcctggatg cattccgccc
     2761 accagacgga cgcacgaacg atgggttcat ccctgcgacc cgtccccgct caccgcacca
     2821 cgcttgagcg agcgtcagcc gatgccgtgg cagccgtgat cggaccaggc gcggccggca
     2881 aacgtagccg gcgctacgcg aagtagatcg ggctggtgag cgcaatcatc caccagccgt
     2941 cgtcggcgac gacctcggcg cgcacgcgcg aatgggtgga ggcatccacg gtccgggtca
     3001 cggtgacgtc ggatccggcg atcggctgca gcgatccttg cacgccgtcg acgacgaagc
     3061 gaacgaagcc ggcgggggcg ccgacgatgc gcgcggcgac ggtgacctcg ccccacgcgg
     3121 cgggaaccac gtcgccgacg atgcgctgct cgcttccctt ttcggcggac aggtagatct
     3181 gcgtgccgcc atacgcgggc ggctgcgcgg acacgaaggt ccggccggcc cggatcgcgt
     3241 cgagaatccc ttgccatgtg ttgctttcgc tgtagaccca ggtggtcggc tggccgacgc
     3301 cttgcgcggc cgcggtcgag cgccaatggt tgtcgctgcc gccggtcgcg ggcacgcggt
     3361 ggccgcggcc gaggaactcg cgatcccacc agggcagcga cagatagttc tccgacgtcg
     3421 agatgccttg gaacgcggga cgccggtagg gccaggcgat gttccacacc tcgaccgaat
     3481 ccggcacgac cgtcgcgggt gagtagcccc atccgggaat gccatcggca ccggtcggcg
     3541 gatggttctt gtagaaggga tggttgagga tgaacagccc tcccgcatcg cggaccgcgt
     3601 cgcgcagcgc gagcgcgccg gcctcggtct gcgtgtcgat cgggaaccgc tggaacacgc
     3661 cgagacagcc ggcatgtccg cggcgcagcg aatgctcgta gcccggcacg agcgtgagag
     3721 catgcccgcc gcccgtggtg ccgtcgccga gccagcgaag atcgtcgtga tcggtgatgg
     3781 cgagatagtc gaggccgcgt gactcggcga tcaggatctg ctcggcggcg gtccagccga
     3841 acgtgtaggc ctcggtttcc gacgtgttgt cgtcgcccgg cgcccacacg tcgccggaat
     3901 agaccgtgtg acagtgcagg tcgccggcga gccattggcc gccggagccc ggctcggcgc
     3961 cggcgagccg cggcagcagc gcccaggacg cgccgagcgc gaccgcgccg cgtagaaagt
     4021 cccggcgcgg gactccgccg ctctcggatg aaggcatgac cgcgagattc gccgcgggac
     4081 gcgccggttc ctgtctcgag gggcggctag ctcgccgctc gctccgactc ggccttcttg
     4141 gtgagcatct cgcccaggcc ttcgtcgggc tgcggccgcg gaatggtcgc ggccgcgacg
     4201 acgatgccgg cggtgatcag caccaggttc ttgaggatga actcgccgag caccgtgagc
     4261 tccagcgggt tgccgccggc gaaggacaag ctcggacgcg cgacgaacac caaaaacgtt
     4321 cccagcatct gggcgaagaa gacgccgagc gtcacccgga tcagccagcc ggtcagcagc
     4381 ccgatcccga ctccgatctc gatcgccccc atcagcacga ccgcggtccg gtcgggcacc
     4441 acgggaatca tgtccgcgac gagcccggcg accgggctga cgccgaacac cttgagcgcg
     4501 ccgaaccaca cgaacaccaa gccgagcgag atgcgcagcg acaggatccc gtaagcgcgc
     4561 gcaagccgga ccgcggttcg gtcggctcga tgcgctcgac gcttgtccat acccggaatc
     4621 tatcccgatc gcggccgggc aaacggcgcg aacctgggcc gtttcgcgtc tcgcggacgc
     4681 ggaagccggt caggacatga cgggcaggga agcacccccg gccgccacgg ccggatcagc
     4741 cgaggaaccg tcgctgccgg gcggcgcgca gcatcgccgg ggcaagatcg gcggtatccg
     4801 ttccccgcgc tccgtcggcg atctccgcgg tgagggcgtg atgctcctcg tccggctcgt
     4861 cgcacaggta cagctcgcgg gcggcacggg cgacctcggg cgggacgaga tccgagtccg
     4921 cgcgcaggta gcggcccgcg cggcgaaggt tgaacatcat ccgcggcgcc gcctcggaca
     4981 ggaacgcgtg gtcgtccttg gtgagcgcct cgatgccgag cacctcgggt ggaaggccga
     5041 ccgagtacag cgcggccgtg aacgagatcg cacgcggcag cgacacaccg gcgacgtcgc
     5101 gtgcgtagcc gaacaaaccg acgtgcagct tgcgggtgcg gcgcgcgggc acgaagcgcg
     5161 cgaggcgctg cacgacgggc gcgagctcga ccacgtgctt gcggtaggcc tgggcggtgc
     5221 ggttgatgat ctcgatgcat cgctcctcgt ccaccgcagg aggcatcgag ggcgtgtgcg
     5281 cgcggatgcg tgcgatcgcg tcgcgcacca tgccaggcgg atggtcgtac ttgaacgccg
     5341 actgcacggt gaaggtgtgc acgctcgggt ggttggccat gatctggtca accgcgtcgg
     5401 gcgtcagtcc cccacggaac ggcgcgctcc ccacacccag gatcggcgca atcttcacgc
     5461 cggaccgttc ctcgagttcg tgcaggcgcc gcaacgcgat cttggccatc aggatcgcag
     5521 cgagcatgcc gtagttcatc gccggatccg agcgggccaa aaacacgcgc tgcgtcgtta
     5581 cgtccttgtc gcgcagatac tcctcggtga tacggtccgc ggcgagcatg ttgtcgagat
     5641 cctcgtagag cgggatcacc tcgatgtgct cgggctcgaa ggacccgacc cactcggcga
     5701 cggtgatgtc gtcgtcgccg agccgctgcg tttgctttcc gaccacgatg tcgcggtagt
     5761 agcggtagat gcggttgagc tcgcgggccg aggtggtcat cgggaggatc acctcgaaga
     5821 tcggcggcgt tgcgccgtcg tagaacggcc gggtcgcgtc ggaggagcgc gcgatgctct
     5881 cgagcgtttc gagcaagatc ttcgcttccg tggtctcgac cgcggggttg ggcacgcgca
     5941 gcgtcagccg gatgtcgcgg ccgagccggt tggcgtcgaa gaacgatgga tacttgccga
     6001 gcagcttctt gacgacgaag ccgtcgacct ccttgccttc cgcgtcccac atctgctcgt
     6061 cgcagcccag atgcgagtac gcgtagtacg cctcctcgat ctcgtcctcg cccgatagcg
     6121 cgggcgcgtc ggcgaagaag ggcggatcga cgttgtcggg atgctgggtg ctcatgcagc
     6181 gcggaacgcc ggtcacccca gcctcctttc ggttctcccc gggcgccgtc cacaccctag
     6241 atgcccgagc cgccggagaa aatgcctcct gagaaggaaa ggccgtccgg caggcagaaa
     6301 tgggtcgcat cggacggact cggggaggtc gcatgcgtcg cgtttcgctg gtgttgatga
     6361 gcctgacttc gctcgccgcg tcgctgctgc cgtcgagcgc gacggccacg ccaggatccc
     6421 atccgggatt tcttttcaac gcgcaggaca accggctcga cgtctacgac ctgtccgcag
     6481 cccatcctgc gctggcacgt tccacgccga ttcacgcgca cgaccacgac ccggatgccg
     6541 ggccgggcga ggatcccgtc ggccgcggca acgacgtcaa cggtcagatc tgccgcgtcg
     6601 tgcaggccga cggctcggtt cgcttcgtga tgggcgagga ctcggaccag gagagccttc
     6661 ccgcgggcat cgcccagggc tgggggctgt tcgagccgtc cgacggggcc gagggtccgt
     6721 ggacgatgac gggcaagatc gtcgcgccgt tcagggttca cgacaacgac catcagcccg
     6781 agacgaccgg ctgcgcggtc tcggccgacg gcagcatgct gttcctggtc gatctcggcg
     6841 tcggcgcgtt cgacgtgccg ggagtcggct cgctgttcct gtaccgccgc gacgccggcg
     6901 ggaacttctc ggcctcgtcg ccggtgtgcg tgctcgccga cgatctgacc accgccggct
     6961 acatcgcgat gcacccggac ggctcggtgc tcgttcctga aggcgggcgc ttcgaaggtg
     7021 gcgtggtcag ccgcttccgg ccgccgttcc cggccgccgg cgacgccgcc gggtgcgctg
     7081 cctaccgcgc cgcgcacggc gtggccgacc gcgagagctt cctcggcccg ttcgcgatgc
     7141 tgccgttcga tcctgtgtcg ttcgttccga tctccatcgc gcgccgcggt tcgggctggc
     7201 tgatcggcaa cgtcttcccc ggccaggtcg ccgaatacga cgggtcggga tcgttcgtgc
     7261 gcgtgatcac cgccggtcag gggccgggcg tcgcgggcat cgccgtggac ggccatggca
     7321 acgtctacat cgcgaacctg ggcctggtgc cgtgcgagac gctgctctgc cccgcgagcg
     7381 gcgccggcac cctatggaag gtcgcgttcg acccgctgac cgacgcaccg cttccgcccg
     7441 tgctcgtgat ggccgggctg acctaccccg aaggaatcgc ggtcttcccg tcgctatagc
     7501 ccggcgttta ggacagacgg accggcaggc gttcgtagcc acgaaggttg atgcgtccgt
     7561 tcacctgcgg ctcgccggca agctcggtgt tcgggaaccg ctccagcagc gagccgatcg
     7621 cgatgcggcc ttcgagccgc gcgagcgccg ctccgaggca atagtggtga ccgccaccga
     7681 aggacaggtt ctgcggcgcc atcgcacgcg ccaggtcgag ccgctcggca tcgccgcccc
     7741 agaacgccgc gtcgcggttc gccgacgcga gcaccagcac gacgaactgg ccctgctcga
     7801 tcgtctggcc gcgtacctcg acctcgcgaa gcgtgatccg gcgagacatc tgcaccggcg
     7861 agtcgaagcg gagaagctct tcgacggcgt tgacgggatc gttcgcgaat cgcgggatcg
     7921 attcgggatt gcgcagcagc gcgcgcacac cgtttccgat caggttaacg gtggtctcgt
     7981 ggccggcgat gaacagcagc atgagctgct cgagcagctc ctcgtcggtc agcacgtcgc
     8041 cttgttcctc ggcggcgatc atcgcgctga gcatgtcagg cgccggatac tgccgcttcc
     8101 atgcgatcac gtccgccagc agcgcccgca tctgatcgcc cgcgtcggcg atcgccacga
     8161 tcagttcggg atcgaccagc ggctcgagcg accgcaccat cagatgcgac caggcgcgca
     8221 cgtcgtcacg gtcggtcggc ggcatgccca gcatctcgca gatgacctcg aacggcaccg
     8281 ggaaggcgag tgtggagatc aggtcgatct cgtcgcccga ggacagcggt tcgagcgcac
     8341 gatcgacgaa ggaccggatc cgcggcgtga gcgactcgat ggtcttgggg gtgaaggcct
     8401 gggagacgag ccggcggagg cgggtgtgat cgggcgcatc gcggttgagc atcgacttgt
     8461 ttccgatctc cgcccgctct cccaccagcg cctcgatcat ctgcgtgacc ggcgtcggac
     8521 gctgatgacg ctcctccacc gacagcgcgg ggtcccgcag gaagtcgacg acgtcgtcgt
     8581 gacggaacag cacggtgaag ccgagcggcg tggtgtacac ggggtacgtg tcgcgcagcc
     8641 gcgcgtactc ggggtaggga tcggcgagaa acgccggatc gaacggattg aagccgtcat
     8701 ccggcggggc agggagcgac tcgagaaggg tggctccggg ctcagagagt tcctcgttcg
     8761 tgccaggcat tccaggcctc cgatgaaagt cggaccggtt cgctcttggt gcggatgcgg
     8821 gcgccgatcg cgaccacgga atcccgcagt gcggaaaaaa ccaggcccag gatccgcagg
     8881 gccgggcggg ccggcgtgtg cagtcgtcga gatgcggtcg ccataacgtc cgcaccatac
     8941 tatgccggtt aggactatgt caagacggaa tatcctagac tgtcatagcc gctgaccagc
     9001 acaaacttgc ccagcagcct tgtgcggtcc ggcccggact attacagttc gggctgtgac
     9061 ccccttaaag aagtccctgc cgacgacctc gtatgcggtg ctcggaattc tgagcatcca
     9121 cccgatgtcg ggctacgagc tcgcgcaggc ggccgagcgg tccatcgcca acttctggcc
     9181 gatctctcgc agtcaggtgt actccgagct cgcacggctc gaagacctcg ggctcatcgc
     9241 cggcgcggac gtcgcccagg aacgcgtgcc ggacaagcgg gtcttcgaga tcaccccggc
     9301 cggcgaggat gcgttcaccg aatgggtctc cgcacccggc tacgagccca cgcgcatgcg
     9361 tcttggcttc tgtctcaaga ccttcttcgg ccaccacatg cctcgcgaga cgatggtcga
     9421 gaacctcgag cgcttcaaga aggagaacga ggagcagatc gcctacctgc agcgcgtggt
     9481 taacatgctc ggcgtgctgc ccgaggccgc cttcacccgt gcgaccgccg agctcggcct
     9541 gcgtatcgcc cggacgtccg cgcagtgggc cgaagagctg ctggccaacg tacccgagat
     9601 ccgccactcc tcgggcgagg agcacgagga agtccaccgc atcgcccgcg agctgttccg
     9661 caaagtcggc cggtagacct gcttttcgcc tgagggccgc gcccgtccgg tccggtaaca
     9721 ccgcaaggcg atgcagacgc tccggacgcg cgccctggtg ccgctccggt cagccgccgt
     9781 gctcgtgcgg ctccgaggtc atcgtgccga gccggatccg gatgcacccg aagcacgatg
     9841 cctcgggaca gtccacgcga tcgcagaact cgcagcactc gatgctgtcg ccacaatgca
     9901 cgcaacgggc ttcgcgctcg tcgctcattc gtcgtctccg tgctcgtgca gatacgtcgt
     9961 cacgtcgtct cgatacagca gcccgaccag cgtgccgtcc gaacgggtca ccggtgagct
    10021 ggtcaggtca tgcttgacca tgtaccgagc catctcgccg gccatcacgt ggggccggaa
    10081 cgtgctcggg cccgggcgca tcacgtcgcc gacgatcgcg ccggcgtcgc gcgcgagctc
    10141 ggcggatcgc aggagcccga ataccacgcc cgcgtcgttg accacgacgc atgcatcgaa
    10201 gccggctgcc gcgacgcgcg cggcgacgtc gcccaacggc tcgtccacgc ggcatgtggg
    10261 gacatcgtga cgcgcgaccg ctcctgcgcg cgccttggcc gggttcgttc cttcagtcgg
    10321 attcccggcc gccagccagt ccagcttgcc ggccccgtag tcgaacacct cggtgaagcc
    10381 gaacgcttcg agccgccacg cggcccgtgg gctgaggtct caggcggagt cccagcagta
    10441 gacgatcacg gccctcgtgc gatcgagcgc ggcgcctgcg gactcctcga gctttcgcaa
    10501 cggcaagctg atcgcgcccg gcaggtgatc c
//
LOCUS       DATLFO010000068         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_110893, whole genome shotgun sequence.
ACCESSION   DATLFO010000068 DATLFO010000000
VERSION     DATLFO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110893"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VM841_02335"
     CDS             complement(<1..184)
                     /locus_tag="VM841_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89052.1"
                     /translation="MGEGERRVAPPDDSVRLQACPPSHPLPPAAKAARSDGGPVPSRT
                     DLKMMRRALTLAERGRG"
     regulatory      complement(169..300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(355..1281)
                     /locus_tag="VM841_02340"
     CDS             complement(355..1281)
                     /locus_tag="VM841_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL89053.1"
                     /translation="MTDVILIADDDADIRRFVELNLRTEGFEIVTAADGEEALSLAFE
                     RNPSLILLDVMMPKMDGFDVCRQLRSDFRTSATPVIMLTARSLSADKVVGLTAGADDY
                     IIKPFDPLELIARVRSTLRRVREMRSTSPLTGLPGNLRIDVEIRARASKGQAFAVCYA
                     DLDNFKAYNDHYGFMRGDAVIRHTAGLIQASAAAVAPDAFVGHIGGDDFIVVCDAEQA
                     EPISEAIIERFDVSVRTLYDEVDASAGGIATVTRTGDRVTYPLLSISIGVSMSLDGAG
                     IEPRALVDAATSMKSLAKQSEGSTLRVDRRRS"
     gene            complement(1295..1972)
                     /gene="rpe"
                     /locus_tag="VM841_02345"
     CDS             complement(1295..1972)
                     /gene="rpe"
                     /locus_tag="VM841_02345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVL89054.1"
                     /translation="MRYKLAPSILSADFMRLGEEISAVAPFSDVIHVDVMDGHFVPPI
                     TIGPVVVQSIRGASDLPLECHLMVDEPEKQIEQFAEAGGSSVVWHLEASGEDPRPILD
                     RARALGLGAGLAINPETSFDDVARYAEHLDTLICMTVTPGWAGQKFRDDVLPKIDSAR
                     AFFSSHGLTADIAVDGGVNLDTGRQCLDAGANVLGAASSIFGAADSAAAAKALRALLD
                     EYESPGG"
     gene            complement(1982..2929)
                     /gene="fmt"
                     /locus_tag="VM841_02350"
     CDS             complement(1982..2929)
                     /gene="fmt"
                     /locus_tag="VM841_02350"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVL89055.1"
                     /translation="MRVAFFGTPDVAAVALRRLIGSHHEIVVAVTQPDRPRGRSGTPQ
                     PPPAKQVAAEAGIPVLQPPTPKQDSFAPEFAAFSPDACVVVAYGHLLPPAVLAVPPRG
                     TYNAHFSLLPRYRGAAPVQRAIMNGDDRTGVCVFLLEPTLDTGPVLERVEVAIEDDDT
                     TGTLLARLAPIGADALVRTLDAVEDGTAEPKPQRDEAATPAPKIKPDEGEIDWSHPAE
                     RIRNLIRGLNPSPGAYTFFKGKRVKIWSAALADEPGEPGEPGAAIPVAGGFAIAAGEG
                     AIVPTELQPEGKRRMTADEFLRGYRPGPTTRFGRDAADR"
     gene            complement(2929..3459)
                     /gene="def"
                     /locus_tag="VM841_02355"
     CDS             complement(2929..3459)
                     /gene="def"
                     /locus_tag="VM841_02355"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVL89056.1"
                     /translation="MGALDIRIWGDPVLRTKARPVEAFDDALRKLAHDMLESMRAAPG
                     VGLAAPQVGVDRRMFVYDSGERFGALCNPAIVWMSGETQEGEEGCLSIPGVYFPVVRA
                     MHVRVEAQDVFGNHVVEEVSDFEARIFQHEIDHLDGILFVDRLEEDQRKEAMRAIREM
                     LLAGEQPGGSSPARAL"
     gene            complement(3473..>3674)
                     /locus_tag="VM841_02360"
     CDS             complement(3473..>3674)
                     /locus_tag="VM841_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89057.1"
                     /translation="SEEIAALLEAGGGTVLGPVKRDGHALLAVRTESRKTLEALLAPL
                     AAAWRDEGTEVRIDIDPREVLA"
BASE COUNT          546 a         1250 c         1299 g          579 t
ORIGIN      
        1 agccgcggcc acgctcggcc agggtcaacg cccggcgcat catcttcagg tcggtccggc
       61 tcggcacagg tcctccatcg cttcgtgcgg ccttcgcggc gggcgggagg ggatgcgacg
      121 gcgggcacgc ctgcagccgg accgaatcgt ccggcggcgc aacgcgccgc tcgccttctc
      181 ccatcccgac tgtacggtcg gccccggaat tccgccgggt cagccggcgc atacctattg
      241 cgcgccgggt cgcgggctat gaccgccggt ggggactcgc accccgcccc gaaggctttc
      301 ggcaccagta taccgggccc ggccacaagt tactaaccag taacttggtc tcggtcagga
      361 gcgccgccgg tcaacgcgga gtgtgctccc ctcgctctgc ttagccagcg atttcatcga
      421 tgtcgccgcg tccaccagcg ccctcggctc gatcccggcc ccgtccagcg acatcgagac
      481 cccgatcgag atcgacagca gcggatacgt gacccgatcc ccggtccggg tcacggtggc
      541 gatgccgccg gcggaggcgt ccacctcgtc gtagagcgtc cggacgctca catcgaatcg
      601 ctcgatgatc gcttcggaga tcggctcggc ttgctccgcg tcgcagacca cgatgaagtc
      661 gtcgcccccg atgtgcccga cgaaggcgtc gggcgcgacc gcggcggccg aggcttggat
      721 gaggccggcc gtgtggcgga tgaccgcgtc gccgcgcatg aatccgtagt ggtcgttgta
      781 ggccttgaag ttgtccaggt ccgcatagca gacggcgaac gcctgtccct tcgaggcacg
      841 agcgcgaatc tcgacgtcga tgcgcaggtt tcccggcagg cccgtcagcg gcgaggtgga
      901 gcgcatctcg cggacccggc gcagcgtgga gcggacgcgg gcgatgagct cgagcggatc
      961 gaacggcttg atgatgtagt cgtcggcccc agcggtaagg cccacgacct tgtcggccga
     1021 tagcgaacgg gcggtgagca tgatcaccgg cgtcgccgag gtccggaagt cgctgcgcag
     1081 ctgccggcac acgtcgaatc cgtccatctt cggcatcatc acgtcgagca agatcagcga
     1141 ggggttgcgc tcgaacgcaa ggctcagcgc ctcctcgccg tcggcggcgg tcacgatctc
     1201 gaagccctcg gtgcggaggt tgagctcgac gaaacggcga atgtctgcgt cgtcgtcggc
     1261 gatgaggatg acgtcggtca cgcgacggct ccggtcagcc gccgggcgat tcgtactcgt
     1321 cgaggagcgc gcgaagggcc tttgccgcgg cggcgctgtc ggccgcgccg aagatggagc
     1381 tggcggctcc cagcacgttc gcgcccgcgt ccaggcactg ccggcccgtg tccaggttca
     1441 ccccgccgtc cacggcgatg tcggcggtca ggccgtgcga gctgaagaac gcgcgggcgg
     1501 aatcgatctt gggcagcacg tcgtcgcgga acttctggcc cgcccagccg ggcgtgacgg
     1561 tcatacagat cagcgtgtct agatgctcgg cgtagcgggc gacgtcatcg aaggatgtct
     1621 ccggattgat cgccagcccg gcgccgagac cgagcgcgcg cgcccggtcc agaatcgggc
     1681 gcggatcctc gcccgaagcc tccagatgcc acacgacgct cgagccgccc gcctcggcga
     1741 actgctcgat ctgcttctcg ggctcgtcca ccattaggtg gcattcgagc ggcaggtccg
     1801 atgcgccgcg gatcgattgc acgaccacgg gaccgatcgt gatgggcgga acgaaatgcc
     1861 cgtccatgac gtcgacgtgg atcacgtccg agaacggggc gacggccgag atctcttcgc
     1921 cgaggcgcat gaagtcggcc gataggatgg atggagccag cttgtatctc acgcgatgat
     1981 tctatcggtc cgccgcgtcg cggccgaagc gggtcgtggg gccgggccgg tagccgcgca
     2041 ggaactcgtc ggcggtcatg cgccgcttgc cttcgggctg cagctcggtg ggcacgatcg
     2101 cgccctcgcc cgcggcgatc gcgaagccgc cggccaccgg gatcgcggcg ccgggctcgc
     2161 cgggctcgcc gggctcatcc gccagggccg ccgaccagat cttgacccgt ttgcccttga
     2221 agaacgtgta ggcgccgggg ctcgggttga gtcctcggat caggttgcgg atgcgctcgg
     2281 cggggtggct ccagtcgatc tcgccttcgt cgggcttgat cttgggagcc ggcgtcgcgg
     2341 cctcgtcccg ctgcggtttc ggctcggcgg ttccgtcttc gaccgcgtcg agggtgcgaa
     2401 cgagcgcgtc ggcgccgatg ggcgcgagcc gtgcgagcag cgtgccggtc gtgtcgtcgt
     2461 cctcgatcgc gacctcgacc cgttccagaa ccggcccggt gtcgagcgtg ggctcgagca
     2521 ggaagacgca tactcctgtg cgatcatcgc cattcatgat cgcgcgctgc acgggtgcgg
     2581 cgccgcgata gcgcggcaga agcgagaaat gtgcgttgta ggtgccgcgc ggggggaccg
     2641 cgagaaccgc cggcggcagc agatgcccgt aggccaccac gacgcacgcg tccggggaga
     2701 aggcggcgaa ctcgggcgcg aagctgtcct gcttgggcgt gggcggctga aggaccggga
     2761 tgccggcttc ggcggcgacc tgtttggcgg gaggcggctg cggtgtgccg cttcggccgc
     2821 gcggccggtc gggctgggtg acggcgacga cgatctcgtg gtgcgagccg atgaggcggc
     2881 gcaaggccac cgcggcgacg tcgggagtgc cgaagaaggc gacgcgcact acagcgcgcg
     2941 ggccgggctc gacccgccgg gctgctcgcc ggcgaggagc atctcgcgga tcgcgcgcat
     3001 cgcttccttg cgctgatcct cctcgagccg gtccacgaac aggatgccgt ccagatgatc
     3061 gatctcgtgc tggaagatcc gggcttcgaa gtcgctgacc tcttccacca catggttgcc
     3121 gaagacatcc tgggcctcga cgcgcacgtg catggcacgc acgaccggga agtacacgcc
     3181 tgggatcgac aagcagcctt cttcgccttc ctgcgtctcg ccgctcatcc atacgatagc
     3241 ggggttgcac agcgcgccga atcgctcgcc ggagtcgtag acgaacatgc gccggtccac
     3301 gccgacttgc ggcgcggcca ggccgacgcc cggcgcggcc cgcatgctct cgagcatgtc
     3361 gtgggcgagc ttgcgcaggg cgtcgtcgaa tgcctcgacc ggccgcgcct tcgtgcgcag
     3421 caccggatcg ccccaaatac gaatgtcgag cgcgcccatg acgcggtcga gtctacgcaa
     3481 gaacctcgcg cgggtcgata tcgatacgca cctcggtgcc ctcgtcgcgc catgcggcgg
     3541 cgagcggcgc gagcagcgct tcgagggtct ttcgagactc cgtccgcacg gcgagcagcg
     3601 cgtggccgtc ccgcttcacc ggcccgagga ccgttccccc gccggcctcg agcaacgcgg
     3661 cgatctcctc gctc
//
LOCUS       DATLFO010000069         5028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_11181, whole genome shotgun sequence.
ACCESSION   DATLFO010000069 DATLFO010000000
VERSION     DATLFO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11181"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1223)
                     /locus_tag="VM841_02365"
     CDS             complement(147..1223)
                     /locus_tag="VM841_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018731028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL89058.1"
                     /translation="MSSHPLYTTPVTEDQWEIPGTFETVFNWEYDDQRDRLVSLYKKG
                     KDKQWDAAERIDWSLEVDPENPSQMPDEANIGIYGSEIWERMTEAEKVELRRHVEAWR
                     ASQFLHGEQGALICSSKIVQAVPDIDSKFYAATQVIDEARHVEAYSRYVHTKVKLAYP
                     INPNLRTLLEQVIRDSRWDFTYLGMQVLIEGLALAAFGLIRDAAQNPLAASINAYVMQ
                     DEARHVAFGRLALRDYYPQLTQAERDEREDFVVEACYLMRDRFKAEEIWENVGLPVRE
                     CAQYVDTSERMQMFRNILFMRIVPIVKDIGLWGEKVQKAYADMGVLDFASIDPDVAAA
                     QDEDIAEQFDLRRRQIEDTIAAAE"
     gene            1493..3076
                     /locus_tag="VM841_02370"
     CDS             1493..3076
                     /locus_tag="VM841_02370"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL89059.1"
                     /translation="MRGFDPRRAAALFLGTAVVLGLTVAIPSPPVVCEAPPAPVFGDM
                     AIIDEERAGGEPIIATHPDGTLLYGAHAGTTHVYGSEAGGGTDPFWRHYDNQAYYYWS
                     DDHGKTWTFVPRDLPDNVDGSGFSDPDFAIDTAGNVFISEINLLNVAVSASADSGRSY
                     ELRNFFGQDVADRQWSEADEKDVVYMVGNVFGGGTSTSPAGKVGHTLFKSKDGGRTFT
                     GGLPDAGGLGDLRVDKSDGTLYETHLETSGEKKLSMAAFRKIRRDDFTVEKNVIAKGV
                     DMLAHWPSFDLDPSGNLYVTWDESGRGARPAGVWFSASKDRGKTWSLPQRLDRDERTD
                     TWPWLAVGDDGRVAVAWLEADEALPDHDAETPGEHGWNIMVAATTSGLGCDASPIPRF
                     TVVKATREPIHTGTICQGGTICQALGIDRRLGDYFSIEIDAAGRMVAAYSDTRRGGLV
                     ALPGFLRQVDGVSFFAPAEPSIKPAPIKPPAAPGSKPVVRGVRNELPATGVEDTGLAA
                     GLVLLSLACVAARRLTAHR"
     gene            3130..3669
                     /gene="rfbC"
                     /locus_tag="VM841_02375"
     CDS             3130..3669
                     /gene="rfbC"
                     /locus_tag="VM841_02375"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HVL89060.1"
                     /translation="MEYVESVEELKQISGVYLVRLKEFGDERGRFIETWRRAWVPGAR
                     EMVQTNRSDSRAGTLRGMHYHLFQADYWNLVSGRAIAGLYDFRASSPTSGQSATIELA
                     DDVGVYIPPGVAHGFYAVTDATMTYFVDQVYDGSDELGFRWDDPALQIDWPDGERILS
                     ERDQIAPLLADVEDENRPD"
     gene            3835..>5028
                     /locus_tag="VM841_02380"
     CDS             3835..>5028
                     /locus_tag="VM841_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89061.1"
                     /translation="MRLRTVFTGLMTGLLLVGSVWAGVGLSPVDRLVDPAADAVPQQV
                     GARFASLQVGTGATLGADEIFVGASKISLEPTPDASQGEVWVRDKKACLPATSGDPMA
                     GQAHVADWRSPWIENSNCIYMGGYGIGPTNPILTWDDHYGLWVRTAVFADAAGDTLVL
                     TLLDAEGYFGEYNNMCGTAEPRCGAFDIADDLGAELGISPDGFIIASTHAHTALDLIG
                     GWGGVPGWYMDQVAGAIKDSIRQAFANKAPARLEAGEQLAREFNGERRSKYRSAEDPT
                     INWIRAIGRDNNVITTVSTFATHPVNYDEELGIGHADFPVAFAHQAEANQGGGVAMVF
                     QAGLGNMTGGGSWEPKGKGLANRLPAVGLGTPVPTPDVRVKREFWNQPVTNVPLGSLG
                     AGGFFD"
BASE COUNT          831 a         1677 c         1726 g          794 t
ORIGIN      
        1 ctcacgggag gcagcgtggc gttggcctcg ctgcggatca cctgggaggt ggcgccgaac
       61 agcacgagcg ccgacaccag cgcgatcgga agcggacggg cgtggcggtg cagccacgcg
      121 agacgcgagc gaatccggga gcccatctac tcggcggccg cgatcgtgtc ctcgatctgg
      181 cgacggcgca gatcgaactg ctcggcgatg tcctcgtcct gcgccgcggc aacatcggga
      241 tcgatcgagg cgaagtcgag cactcccatg tcggcgtagg ccttctgcac cttctcgccc
      301 cacaagccga tgtccttgac gatcggcacg atgcgcatga acaagatgtt gcggaacatc
      361 tgcatacgct cggaggtgtc gacgtattgc gcgcactcgc gcaccggcag gccgacgttc
      421 tcccagatct cctccgcctt gaagcggtcg cgcatcaggt agcaagcctc gacgacgaag
      481 tcctcgcgct cgtcgcgctc ggcctgggtg agctgcgggt agtagtcgcg aagcgcgagc
      541 cggccgaaag ccacgtgccg cgcctcgtcc tgcatcacgt aggcgttgat ggacgccgcc
      601 agcgggttct gcgccgcgtc gcggatcaac ccgaacgcgg ccagcgcgag cccttcgatc
      661 agcacctgca tgccgagata agtgaagtcc caacgtgagt cgcggatgac ctgctcgagc
      721 agcgtgcgca ggttcgggtt gatcggatag gccagcttca ccttggtgtg cacatagcgg
      781 gagtaggcct ccacgtggcg ggcctcgtcg atgacctggg tggccgcgta gaacttggag
      841 tcgatgtcgg gcacggcctg gacgatcttg gacgagcaga tgagcgcgcc ctgctcgccg
      901 tgcaggaact gggaggcgcg ccaggcttcg acgtggcggc gcagctcgac cttctcggcc
      961 tcggtcatgc gctcccagat ctcggagccg tagatgccga tgttggcctc gtcgggcatc
     1021 tgcgacgggt tctcgggatc gacctcgagg gaccagtcga tgcgctccgc cgcatcccac
     1081 tgcttgtcct tgcccttctt gtacaacgag acgagccggt cgcgctggtc gtcgtactcc
     1141 cagttgaaga cggtctcgaa cgtacccggg atctcccact ggtcttcggt gacgggcgtg
     1201 gtgtacagcg gatgactgct cattcaactc cccccaggcg tcgcctgggc cgagggtacc
     1261 actagggtgc gcccaggcac cacggcgcga aatcgccgct tcgatgttcg cgagcacacg
     1321 gcggtgaccg accgggccga atcgagcgtt cgcgcccgcg tccccggggc cccgggtgat
     1381 atacctggac cgttcgtggg cgaaacgccg aatccatacg atttggggcg tcccggcagg
     1441 ggtacgcgga gaggcgagga cttccggcga acggcggcga aatgagggga ctatgagagg
     1501 cttcgatcca cggcgcgcag cggcactatt cctgggaacg gcagtggtcc tcggcttgac
     1561 ggttgcgatc ccgtcgccgc cggtcgtgtg cgaggcgccg ccggctccgg ttttcggcga
     1621 catggcgatc atcgacgagg agcgcgccgg cggcgagccg atcatcgcga cgcatccgga
     1681 cggcacgctg ctgtatggcg cgcacgccgg aaccacgcac gtgtacggct ccgaggcggg
     1741 gggcggcacg gatccgttct ggcggcacta cgacaaccag gcctactact actggagcga
     1801 tgaccacggc aagacgtgga cgttcgtgcc gcgcgacctg cccgacaacg tggacggcag
     1861 cggcttcagc gaccccgact tcgccatcga caccgccggc aacgtcttca tcagcgagat
     1921 caacctgctc aacgtcgccg tctccgcttc ggcagactcc ggccgcagct acgagcttcg
     1981 caacttcttc ggccaggacg tcgccgaccg tcagtggagc gaggccgacg agaaggacgt
     2041 ggtgtacatg gtcggcaacg tctttggagg cggcaccagc acgagccccg ccggcaaggt
     2101 cggccacacc ttgttcaagt ccaaggacgg cggccgcacg ttcaccggcg gccttcccga
     2161 cgcgggcggc ctcggcgatc tgcgcgtgga caagagcgac ggcacgctct acgagacgca
     2221 tctggaaacc tcgggcgaaa agaagctttc gatggcggcg ttccgcaaga tccgccggga
     2281 cgacttcacc gtcgagaaga acgtgatcgc caagggcgtg gacatgctgg cgcactggcc
     2341 gtcgttcgac ctggatccga gcggcaacct ctacgtgacc tgggacgagt ccggccgggg
     2401 cgcccggcct gcgggcgtgt ggttctcggc ctcgaaggac cgcggcaaga cctggtcctt
     2461 gccgcaacgg ctcgaccgcg acgaaaggac cgacacctgg ccgtggctcg ccgtcggcga
     2521 cgacggccgc gtcgccgtcg cgtggctgga ggccgacgag gcgctgccgg atcacgacgc
     2581 ggagacgccc ggcgagcacg gctggaacat catggtcgcc gcgacgacca gcgggctcgg
     2641 ctgcgacgcg tcgccgatcc cgagattcac ggtcgtgaag gccacccgcg agccgatcca
     2701 cacaggcacg atctgccagg gcggcacgat ctgccaggcg ctgggcatcg accgccggct
     2761 cggtgactac ttctcgatcg agatcgacgc ggccggccgc atggtggcgg cgtactcgga
     2821 cacgcgccgc ggcgggctgg tcgcgctgcc cgggttcttg cgtcaggtgg acggggtgtc
     2881 gttcttcgcg ccggccgagc cgtcgatcaa acccgccccg atcaagcctc cggcggcccc
     2941 gggatccaag ccggtggtca ggggcgtgcg gaatgagctg cccgcgacgg gggtcgaaga
     3001 caccgggctt gctgccggac tcgtcctgct ttcgctcgcc tgcgtcgcgg ctcggcggct
     3061 caccgcgcac cgataggatt tcgtccgcgg ccgagcggac ccgtcggggc tcgactacag
     3121 tcttccggca tggaatacgt cgagtccgtc gaagagctca agcagatctc cggtgtgtat
     3181 ctcgtccgtc tcaaggagtt cggggacgag cgaggacgat tcatcgagac ctggcggcga
     3241 gcgtgggttc ccggcgcgcg tgagatggtt cagaccaacc gcagcgacag ccgggccgga
     3301 acgctgcgtg gcatgcacta ccacctgttc caggccgact actggaatct ggtctcgggc
     3361 cgcgcgatcg cagggctcta cgacttccgc gcctcctcgc cgacgtcggg ccagtcggcc
     3421 acgatcgagc tggccgacga cgtgggcgtc tacatcccgc cgggcgtcgc gcacggcttc
     3481 tacgccgtca ccgacgcgac catgacctac ttcgtggacc aggtctacga cggcagcgac
     3541 gagctcgggt tccgctggga cgatccggcc ctgcagatcg actggccgga cggcgagcgc
     3601 atcctgtccg agcgcgatca gatcgcaccg cttctcgccg acgtcgaaga cgaaaaccgc
     3661 cccgactagt cccattagca ggtgaagccg cgccgagacg gccgcgccgc cttcccggcc
     3721 gcgcgatgcc cttcccgaac aacgggcatc accgaattgg gcctccaaag agggttttga
     3781 cggtttcctg cagaactacc gctgcgaccc cgtctcgtcc tcaggaggga tccaatgcga
     3841 cttcgcaccg tgttcaccgg actcatgacc ggcttgctgc tcgtcggcag cgtctgggcg
     3901 ggggtcggtc tttcgcccgt ggaccggctg gtggatcctg cagccgatgc cgtgccgcaa
     3961 caggtcgggg cccggttcgc ttcgctgcag gtcggcacgg gagcgacctt gggcgcggac
     4021 gagatcttcg tgggggcgtc gaagatcagt ttggagccga cgccggatgc gagccagggc
     4081 gaggtctggg tgcgggacaa aaaagcttgc ttgccggcca cgagcggcga cccgatggcc
     4141 ggccaggcgc atgtcgccga ctggcgcagc ccctggatcg aaaactcgaa ctgcatctac
     4201 atggggggct acgggatcgg tccgacgaac ccgatcctga cgtgggacga tcactacggc
     4261 ctgtgggtgc gcacggcggt cttcgcggac gcggcgggcg acacgctcgt gctgacgctg
     4321 ctcgacgccg aaggctactt cggcgagtac aacaacatgt gcggcaccgc cgagccgcgc
     4381 tgcggcgcgt tcgacatcgc cgacgatctg ggggcggagc tcgggatcag cccggacggc
     4441 ttcatcatcg cctcgacgca cgcgcacacg gcgctcgatc tgatcggggg ctggggcggc
     4501 gtgcccggct ggtacatgga tcaggtcgcc ggcgccatca aggactcgat ccgtcaagcc
     4561 ttcgcgaaca aggctcccgc tcgtctggag gcgggcgagc agctcgcgcg cgaattcaac
     4621 ggtgaacgcc gcagcaagta ccggtcggcg gaagacccga cgatcaactg gatccgcgcg
     4681 atcggacgcg acaacaacgt catcaccacc gtcagcacgt tcgcgaccca tccggtgaac
     4741 tacgacgagg agctcgggat cggccacgcc gacttcccgg tcgctttcgc gcaccaggcc
     4801 gaggccaacc agggcggcgg tgtcgcgatg gtcttccaag ccggtctcgg caacatgacc
     4861 ggcggcggct cgtgggagcc caagggcaag ggtcttgcga accggcttcc cgccgtgggc
     4921 ctgggcacgc cggtccccac gcccgacgtg cgggtcaagc gtgagttctg gaaccagccc
     4981 gtgaccaacg ttccgctcgg ctcgctgggt gcgggcggct tcttcgac
//
LOCUS       DATLFO010000070         6244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_80952, whole genome shotgun sequence.
ACCESSION   DATLFO010000070 DATLFO010000000
VERSION     DATLFO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80952"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VM841_02385"
     CDS             <1..761
                     /locus_tag="VM841_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HVL89062.1"
                     /translation="ARTRYEQRGGGTETTTERQIAYSPEIPGPRPGEARSEWEIFMQA
                     AQRAYPERAAQIAFPSGAAIRDEIAKTVPLYAGIERLRKKGDHVQWGGRLLCEGGRFP
                     TEDGQARFVAVAPPESRLKNAGFLLSTRRGKQFNSMIHRERDPLTGALREDVFMAKTD
                     AEALGLAHGDPVLVRSDRGEYRGRVKIAAIKPRNVQVHWPESQALLHRGVRDPECGVP
                     DYNAVVTIEPDADRRAAPGVRASGSLHETDEGSR"
     gene            799..1305
                     /locus_tag="VM841_02390"
     CDS             799..1305
                     /locus_tag="VM841_02390"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HVL89063.1"
                     /translation="MAGNRYTLKDIVQGKPMGHPSHPMFVHFPIAFYVGALGFDVLSR
                     VGSFESAPLAATWLIAGAALFLPVLLLTGLVDWSGMRPGGRARKMANKHMLLQLSAAA
                     LFIVTLILRWPDREIAEAKTMWIALEAVGVVAMGIGNYFGGLLIYKMSMRVGETPPSK
                     TIEEPVAS"
     gene            1305..2159
                     /gene="fdhD"
                     /locus_tag="VM841_02395"
     CDS             1305..2159
                     /gene="fdhD"
                     /locus_tag="VM841_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HVL89064.1"
                     /translation="MIGGEPASRRPGATTPVTPVVIGADGSRRPRSDVVATEEPLEIR
                     VAHRGREHQVAVTMRTPGHDFELAAGFLLGEGMVRERRHVGKVTYCASGPPEQLYNLV
                     TVELANAAPFDADAMRRNMFTSSSCGVCGKASIEAVRSGVPVTLPAGPVVDAATLLSL
                     PDTLRTKQKVFERTGGLHAAGLFDAQGTPLLVREDVGRHNAVDKVAGALLLAGGIPAG
                     ERILQVSGRASFEIVQKAAMAGIPIVAAVSAPSSLAVELADETGMTLIGFSRESTLGV
                     YTRPERIA"
     gene            complement(2196..3251)
                     /locus_tag="VM841_02400"
     CDS             complement(2196..3251)
                     /locus_tag="VM841_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HVL89065.1"
                     /translation="MSNGSHSGPARIKIICTLGPASMDPAVIRGLDTCGVDLLRINLS
                     HTPVRDLEKTVDFVRRHTSLPLCLDTQGAQVRSGAVAPDVVLTEGSSVELTAEAVLGT
                     PERISLWPVSVFDVLSEGSAIGIDSYGAALRVVEAASRGARAVVIRSGRVGSNKAVTV
                     DPPPPLPALTAADVAAIEVGHRTGIMSYALSFASHAEAVRRARGLIPAGATLISKIES
                     RLGVLNMDGVIEASDAVLVDRGDLSREVPLESVPYFQKTILRRANRFRTPSYVATNLL
                     ESMVVSARPTLAELNDIANTLLDGAHGLVLAAETAIGVDPVGVVELVRRAIDMFERQD
                     DGPLLLLKAASQRVAAR"
     gene            3406..4296
                     /locus_tag="VM841_02405"
     CDS             3406..4296
                     /locus_tag="VM841_02405"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVL89066.1"
                     /translation="MSEPQGRLPTFLIIGAMKCGTTSLYHYLREHPGVFMPEVKAPEF
                     FAGGAHWRRGIDWYRGLFAAAKPGTRALGEASNVYAKYPRYRGVPERIALAVPGVKLI
                     YAIRDPVERIRSHWQTRASEGSERRPLHEAAFADPIYLDYSRYAMQIEQYLPVFPREQ
                     IMVIRAEDLRDDRIGTLARVYEFIGVDPAFVPPNVERAFYRTGDRAARSPVPVWLRKG
                     LKRHLPATKRAKELENNIVGAIRRASGRQPLRAEVPPTTRARILDALAGDRRRLEALL
                     PDHPTRPETFDDPLRRGAAR"
     gene            4265..5845
                     /locus_tag="VM841_02410"
     CDS             4265..5845
                     /locus_tag="VM841_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89067.1"
                     /translation="MIRFGEARRASRVPLWLIPLTVAGLLAAAIPAAGSSVVQVAVAT
                     GSDDAEESPAGGVSLTSSDLELVTDGTKVQSVGVRFASLGVPPRATIRSARIQFRTDE
                     VGTDAASLTIAAQAADSAPTFQSVSRDVSSRPRTAASVAWTPAPWTTVGEAGVDQRTP
                     DLTAVVAEVVARPGWTEGNAIAFIITGTGRRTADSLEGRYATSLRIEFDPPSLPGPAG
                     VTFTVGGDYGAAATTVASLAAMDAIPHDFHMANGDFAYSDTATEQEWCDFVLARIPAL
                     GPAFPFELVVGDAETDSQPKKLVNYVACLPDRLSATLSPTNQFGAEYFFDYPPGAPVI
                     RMIAIGADQKVNGIRYRYLSGNARYTWLAATIDGARTAGIPWVAVAMHEVCQSAGKKA
                     CTVGGTLMDLLFAKRVDLILQAHDHNYQRSKQIALDASVCPSAPVGGFDAACVADDGS
                     DDDYLKGAGSVFVINGAFGGRGLSAVSTADPEAGYFAELDSSTRGFSLYTVTATRIEG
                     RFVNSVGSFTDAFVIRAP"
     gene            complement(5842..>6244)
                     /locus_tag="VM841_02415"
     CDS             complement(5842..>6244)
                     /locus_tag="VM841_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HVL89068.1"
                     /translation="PRQGPLVAFDAGSHALAEHGHDGPVILLACDMPLVTGEMLARMT
                     RMRNGADAVVPIDQGRLQPLCAVYAPRARVIAHGLVAAGARSMRDLLTTLRLAIVPAS
                     MFAREGEPDPFSDVDTPGDLERLEPLLESPA"
BASE COUNT          956 a         2167 c         2179 g          942 t
ORIGIN      
        1 ccgctcgcac gcgctacgag caacgcggcg gcggcaccga gacgaccacg gaacgtcaga
       61 tcgcctactc gcccgagatc cccggcccgc ggcccggcga ggcacgaagc gaatgggaga
      121 tcttcatgca ggccgcccag cgcgcctatc ccgagcgcgc cgcccagatc gcgttcccgt
      181 ccggcgcggc gatccgtgac gagatcgcga agaccgttcc gctgtacgcg gggatcgagc
      241 ggctgaggaa gaagggcgat cacgtgcagt ggggcggccg gctgctgtgc gagggcgggc
      301 gtttccccac cgaggacgga caggcccggt tcgttgcggt ggcgcctcct gaatcccgac
      361 tgaagaacgc gggctttctg ctgtccacgc ggcgcggaaa gcagttcaac tcgatgatcc
      421 atcgtgagcg cgatccgctc accggcgcgt tgcgcgagga cgtgttcatg gccaagaccg
      481 acgccgaagc gctcgggctc gcgcacggcg atccggtctt ggtgcgaagc gacagggggg
      541 agtatcgcgg ccgcgtgaag atcgcggcca tcaagccgcg caacgtccag gtgcattggc
      601 ccgagtcgca ggcgctgctc caccgcggcg tgcgtgatcc cgagtgcggc gtccccgact
      661 acaacgcggt cgtgaccatc gagccggacg ccgaccggcg ggcagccccc ggcgtccggg
      721 cttccggcag cctccacgaa accgacgaag gctcgcgcta ggcttgacgg gcatgcccgt
      781 tcgagcgagg gggaacgtat ggccggaaat cgctacaccc tgaaggacat tgtgcagggc
      841 aagccgatgg gtcacccgag ccatccgatg ttcgtgcact ttccgatcgc gttctacgtc
      901 ggcgcgctcg gtttcgacgt gctctcgcgc gtcggctcgt tcgagtccgc tccgctggcg
      961 gcgacctggc tgatcgccgg tgcggcgctg ttcctcccgg tgctgctgtt gaccgggctg
     1021 gtggactggt ccggcatgcg tccgggcggc cgcgctcgaa agatggcgaa caagcacatg
     1081 ctgctgcagc tatccgcggc ggcgctgttc atcgtcacgt tgatcctccg ctggccggat
     1141 cgcgagatcg ccgaggcaaa gacgatgtgg atcgcgctcg aggccgtcgg cgtcgtggcg
     1201 atgggcatcg gcaactactt cggcgggctt ttgatctaca agatgagcat gcgcgtgggt
     1261 gagacgccgc cctccaagac gatcgaggag cccgtcgcgt cgtgatgatc ggaggcgaac
     1321 cggcgtcgcg gcgccccggt gccacgacgc cggtcacgcc ggtcgtcatc ggcgcggacg
     1381 gctcgcgccg cccgcgcagc gacgtcgtcg ccactgaaga gcccttggag attcgcgtcg
     1441 cccaccgggg ccgcgagcat caggtggccg tcacgatgcg cacacccggc cacgacttcg
     1501 agcttgccgc cggttttctg ctgggcgagg gcatggttcg tgaacgccgg catgtcggca
     1561 aggtcacgta ctgcgcgtcc ggccctcccg agcagctcta caacctggtc accgttgagc
     1621 tcgcgaacgc ggctcccttc gatgccgatg cgatgcggcg aaacatgttc acgagctcct
     1681 cgtgcggcgt gtgcggcaag gcgtcgatcg aggccgtccg aagcggcgtc ccggtgacgc
     1741 ttcccgccgg gccggtcgtg gacgccgcga cgctgctgtc gctgcccgac acgctgcgca
     1801 cgaagcaaaa ggtcttcgag cgcaccggcg gcctgcatgc cgccggactg ttcgacgctc
     1861 agggaacgcc gctgctcgtt cgggaggacg tgggccggca caacgcggtg gacaaggtcg
     1921 ccggcgcgct tctgctggcg ggcggcatcc cggcggggga gcggatcctt caagtcagcg
     1981 gccgggcctc cttcgagatc gtgcagaagg ccgcgatggc cggcatcccg atcgtggcgg
     2041 cggtgtcggc cccgtcctcg ctcgccgtcg agcttgccga cgagacgggc atgaccctga
     2101 tcggcttcag ccgcgaaagc acgctgggcg tctacacgcg gccggaacgc atcgcctagg
     2161 cgcaatcgag tgcgcggagg cggaacgcgg gagggctagc gggcggcgac gcgctgggag
     2221 gcggccttga gcagcaacag cggcccgtcg tcctggcgct cgaacatgtc gatcgcccgg
     2281 cgcaccagct cgacgacacc gaccgggtcg acgccgatcg cggtttcggc cgcgagcacc
     2341 agcccatgcg cgccgtcgag cagcgtgttc gcgatgtcgt tgagctcggc cagcgtcggc
     2401 cgggcgctca ccaccatcga ctcgagcagg ttggtcgcga cgtacgacgg cgtgcgaaac
     2461 cggttggcgc gtcgcaggat ggtcttctgg aagtagggca cggattccag gggaacctcg
     2521 cgcgaaagat cgcctcggtc cacgagcacc gcgtccgatg cttcgatcac gccgtccatg
     2581 ttgaggacgc cgaggcggct ttcgatcttc gagatcaagg tggcgccggc cgggatcagc
     2641 ccccgggccc ggcggaccgc ctcggcgtgg gaagcaaacg aaagggcgta gctcataatt
     2701 ccggtgcggt ggccgacctc gatcgcggcg acgtcggccg ccgtcagggc gggaagcggc
     2761 ggcgggggat cgaccgtgac ggccttgttc gatccgaccc gtccgctccg aatcacgacg
     2821 gcgcgcgcgc cgcgggatgc ggcctccacc acgcgcagcg cggctccgta gctgtcgatg
     2881 ccgatcgcgc tcccctccga gagaacgtcg aagaccgaca cgggccagag cgaaattcgt
     2941 tcgggggtgc cgagaaccgc ctcggccgta agctcgacgc tcgatccctc cgtcaagacc
     3001 acgtcgggcg cgaccgctcc ggaacggacc tgggcgcctt gcgtgtccag gcacagcggc
     3061 agcgaggtgt gacgcctcac gaaatcgacg gtcttctcca gatcgcggac cggggtatgc
     3121 gacaggttga ttcgcagaag atccacgccg cacgtgtcga ggccgcggat cacggccggg
     3181 tccatcgatg cgggaccgag cgtacaaatg atcttgatgc gggcaggacc ggagtggctg
     3241 ccgttgctca taccggcagt gtcgctccgc gtccgcgccc cgccatgatg caaacgacgg
     3301 agccgtgatc cgttgagcgc cggatgcgcc gaatgcccac gcccgtcacg ctgtgcccgt
     3361 ccggccgtca cgcggccgga cggtccgtgc gcccgggaga ggtgaatgag cgaaccgcag
     3421 ggcaggctgc cgaccttttt gatcatcggg gcgatgaagt gcggcaccac gagcctgtac
     3481 cactatctgc gcgagcatcc gggtgtgttc atgcccgagg tcaaggctcc cgagttcttc
     3541 gcgggtggcg cgcattggcg gcgaggcatc gactggtacc gcgggctctt cgcggccgcg
     3601 aagccgggta cgcgggcgct cggcgaggcc tcgaacgtct acgccaagta tccccgctat
     3661 cgcggcgtgc ccgagcggat cgcgctcgcc gttcccggcg tcaagctcat ctacgcgatc
     3721 cgtgacccgg tcgagcgcat ccgctcgcac tggcagacgc gggcctccga aggcagcgag
     3781 cggcggccgc tgcacgaggc ggccttcgcc gatccgatct atctggacta cagccggtac
     3841 gcgatgcaga tcgagcagta cttgccggtc tttccgcgcg agcagatcat ggtgatccgc
     3901 gccgaagatc tgcgcgacga ccgcatcggc acgctcgcgc gcgtctacga gttcatcggc
     3961 gtcgatcccg cgttcgtccc gcccaacgtg gaacgcgcct tctaccggac cggcgaccgt
     4021 gccgcgcgct ccccggtccc ggtatggctc cgcaagggcc tcaagcgaca tctccccgcc
     4081 accaagcgcg ctaaggagct cgagaacaac atcgtcgggg cgatccggcg cgcgtccggc
     4141 cggcagccgc tgcgcgccga ggtcccgccc acgactcggg ctcggatcct cgatgcgctg
     4201 gccggcgacc ggcgacggct cgaggccctg ctgccggatc acccgacacg accggagacc
     4261 ttcgatgatc cgcttcggcg aggcgcggcg cgctagccgc gtccccttgt ggctgattcc
     4321 cctgaccgtc gccggtctgc tggccgccgc gattcctgcc gcaggttcct cggtggtgca
     4381 ggtcgccgtc gcgaccggca gcgacgacgc cgaggagagt cccgccggag gcgtgagcct
     4441 gaccagctcg gacctcgagc tggtgaccga cggcacgaag gtccagtcgg tcggcgtgcg
     4501 cttcgcatcg ctcggcgtcc cgccccgcgc gacgatccgc tccgcccgga tccagttccg
     4561 caccgatgag gtcggcaccg acgcggcgtc gctcacgatc gcggcgcagg ccgccgactc
     4621 cgcccccacc ttccagtccg tctcgcgcga cgtgtcgagc cggccgcgaa ccgcagcctc
     4681 cgtggcgtgg acgccggcgc cctggacgac ggtcggcgag gccggggtgg accagcgcac
     4741 cccggacctg acggcggtcg tcgccgaggt cgtggcacgc cccgggtgga ccgaaggcaa
     4801 cgcgatcgca ttcatcatca caggcacggg gcggcgcacc gccgattcgc tcgaaggcag
     4861 atacgccacg agcttgcgga tcgagttcga tccgccttcg cttccgggcc cggccggcgt
     4921 gacgttcacg gtcggcggcg actacggcgc agccgcaacg accgtggcga gcctggccgc
     4981 gatggacgcg attccgcacg acttccacat ggcgaacggc gacttcgcct acagcgacac
     5041 ggccaccgag caggaatggt gcgacttcgt gctggcccgt atcccggcgc tcggcccggc
     5101 gttcccgttc gagctggtcg tcggtgacgc cgagaccgac tcgcagccca agaagctcgt
     5161 caactacgtc gcatgccttc cggatcgttt gagcgcgacg ctcagcccga cgaaccagtt
     5221 cggtgccgag tacttcttcg attatccgcc gggcgcgccg gtcatccgaa tgatcgccat
     5281 cggcgccgat cagaaggtca acgggatccg ctaccgatat ctttccggca acgcgcgcta
     5341 cacgtggctg gcggccacga tcgacggcgc gcgcaccgcc gggatccctt gggttgcggt
     5401 cgcgatgcac gaggtctgcc agtcggccgg aaagaaggcc tgcaccgtgg gcggcacgtt
     5461 gatggatctg ctgttcgcga agcgggtcga tctgatcctg caggcgcacg atcacaacta
     5521 tcagcgcagc aagcagatcg ccctcgacgc ctccgtgtgt ccgtccgcgc cggttggcgg
     5581 cttcgacgcc gcgtgcgtgg ccgacgacgg atccgacgac gactacctca agggcgccgg
     5641 ctcggtcttc gtcatcaacg gcgcgttcgg tggccgcggt ctgtccgccg tcagcaccgc
     5701 cgaccccgag gccggctatt tcgccgagct cgacagcagc acgcgcggct tctcgctcta
     5761 caccgtgacg gcgacgcgta tcgagggccg gttcgtcaac agcgtcggct cgttcaccga
     5821 cgcgttcgtg atccgggcgc cctaggcggg tgactccagc agcggctcga gccgttcgag
     5881 atcgccgggg gtgtccacat cggagaacgg atcgggctcg ccctcgcggg cgaacatcga
     5941 cgccggcacg atcgcaagac gcagcgtcgt gagcagatcc cgcatcgagc gcgcgccggc
     6001 cgcgaccagg ccgtgggcga tgacccgcgc gcggggcgcg tagacggcgc acagcggttg
     6061 gagccggccc tggtcgatcg ggacgacggc gtcggcgccg ttgcgcatgc gggtcatacg
     6121 cgccagcatc tctccggtca cgagcggcat gtcgcaagcg agcaggatca ccgggccgtc
     6181 gtgtccgtgc tcggcgagcg cgtgcgagcc cgcgtcgaac gcgacgagcg gcccctgccg
     6241 gggg
//
LOCUS       DATLFO010000071         5161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_71175, whole genome shotgun sequence.
ACCESSION   DATLFO010000071 DATLFO010000000
VERSION     DATLFO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71175"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VM841_02420"
     CDS             complement(<1..442)
                     /locus_tag="VM841_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HVL89069.1"
                     /translation="MAILLDQSTRVVVQGLTGSEGTFHTKRNIAYGTNVVAGVTPGKG
                     GQTWEGSVPIFDSVNDAVKEQGANASLIFVPARFAADAMLEAADAGVDLIVCITENIP
                     AADMARVYPYVRGKGIALVGPNCPGLISPGKANMGIIPGEITGPG"
     gene            complement(442..1593)
                     /gene="sucC"
                     /locus_tag="VM841_02425"
     CDS             complement(442..1593)
                     /gene="sucC"
                     /locus_tag="VM841_02425"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006887662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HVL89070.1"
                     /translation="MDLFEYQGKELFSKHGVPVPDGKVASSPKQVRAIAEKLGGTVVV
                     KAQVQVGGRGKAGGIKLAKTPDEAVSVAEQIIGLDIKGHKVKRVLVERAGDIKNEFYA
                     SFLLDRAGKGFLGMVSSKGGVDIEEVAATEPDAIAKVPISALTGLQPYHLRRLVYGGG
                     IPADAQKNVMALLQKLFDAFVASDASLVEVNPLVQMGDGSVIALDAKVSIDDNAIYRQ
                     AWTEKLKDVGPASKQEKIAAEKGLNYVKLDGKVGIIGNGAGLTMSTLDVVAEAGGTPA
                     NFLDIGGGANAETMAAGIDLIFSDKKVKSLLVNIFGGITRCDLVAQGILEALQKLKDL
                     KAPIVVRLDGTNADQGRAILEKAGDPRIVPQPDMLSAARKAVELSKGKK"
     gene            1761..2306
                     /locus_tag="VM841_02430"
     CDS             1761..2306
                     /locus_tag="VM841_02430"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HVL89071.1"
                     /translation="MPGTTGLTYQALLAFPDDGLRRELLDGELIVSAAPRLRHQVILG
                     RLHLLFGNFVEEHRIGHVYMAPTDVLLSDDNVVEPDLIFVADGRSEILTEMNMQGVPS
                     LLIEVVSDPRIDRVRKRDVYARFGVPEYWVVDPDADRIEVYCHSPDGYGKPVIFEPGE
                     TLEYEGLPGLQIDLTRLFRRD"
     gene            complement(2303..2698)
                     /locus_tag="VM841_02435"
     CDS             complement(2303..2698)
                     /locus_tag="VM841_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HVL89072.1"
                     /translation="MPVRVVIAKPGLDGHDRGAKVVARALRDAGMEVIYTGLHQTPEM
                     IVEAAIQEDADAIGLSIHSGAHMTLFPRVVELLKERGANDIAVFGGGIIPKEDIPVLK
                     EMGIAEIFTPGTPTQAIVDWINASLATTA"
     gene            2794..4233
                     /locus_tag="VM841_02440"
     CDS             2794..4233
                     /locus_tag="VM841_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVL89073.1"
                     /translation="MREAKPWLDQVLDPGWVERDADLASANPLVFPGYEPGRETVLAA
                     SGALAGHAVEAISFDFSVLGGSMGIVAGEKVARAFERAPGRRASVVALCASGGARMQE
                     GMTALVQMAKTVVARETLARAGLPFVAYLRNPTTGGVYASFASLADVVWAEPDATVGF
                     AGPRVAETVTGRPLPPGSHTAGFAARHGLIDGEIEPGELRARLRALLDLVSGRDHTGP
                     IGTIDEPAAPAEPDAWQQVIFARDRARPDGAAFARRVCADLIEIHGDRRGGGDDDGVL
                     CGVARIGGVRCGVVALARTHPAAAGFRKAQRLIALAGRWRLPVVTFVDTPGADPSSES
                     EAAGIARAIAETFAAVLNHPLPVVSAVTGEGGSGGALALACADRLLIMEHAIFSIIGP
                     EAAAAILKRDDPASVAADLRLTSFDLRRLGIADQIVPEPAPAAHSDPDRAADTLGRAL
                     ALALAEIDPEPARESRTRRWREAGSAFLR"
     gene            4255..4410
                     /locus_tag="VM841_02445"
     CDS             4255..4410
                     /locus_tag="VM841_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89074.1"
                     /translation="MEPKPYKKKRVHPPRKIVPRGRMGIQWIIGPVIIAGVILIAGTI
                     FLIVKPQ"
     gene            4643..>5161
                     /locus_tag="VM841_02450"
     CDS             4643..>5161
                     /locus_tag="VM841_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL89075.1"
                     /translation="MDRNQLSESYRKAQAARRAVTRALRRPRTYRSAVKEIAWAGLNL
                     AMYPAGVVSEALEPNQDRGLAGRFTPELPLRYLDPEAASTPIILLHGYFHNRSAFLVA
                     RRALRRAGFRHISTMNYNVIGHDVEELAQQLSAHVDRVLEETGAHRVHLIGHSLGGLV
                     ARAYIQLCGGREK"
BASE COUNT          790 a         1787 c         1701 g          883 t
ORIGIN      
        1 ggccggggcc ggtgatctcg ccggggatga tgcccatgtt cgctttgccg ggcgagatca
       61 ggccgggaca gttgggaccg accagcgcga tgcccttgcc gcgcacgtac ggatacacgc
      121 gcgccatgtc ggcggcgggg atgttctcgg tgatgcacac gatcaggtcc acgcccgcgt
      181 cggcggcctc cagcatcgcg tcggccgcga agcgcgccgg cacgaatatc agcgacgcgt
      241 tcgcgccctg ctccttcacg gcatcgttga ccgagtcgaa gatcgggacg ctgccttccc
      301 aggtctggcc gcccttgccg ggcgtgacgc cggcgacgac gttggtgccg taggcgatgt
      361 tgcgcttggt gtggaacgtg ccttccgagc cggtgaggcc ctgcaccacg acacgcgtgc
      421 tttgatccag cagaatggcc attacttctt ccccttcgaa agctcgacgg ccttgcgcgc
      481 cgcggacagc atgtcgggct gcggaacgat ccgcggatcg cccgccttct ccaagatcgc
      541 gcggccctgg tcggcgttgg tcccgtccaa gcggaccacg atcggcgcct tcagatcctt
      601 cagcttctgc aacgcctcca agatgccttg cgcaaccaga tcgcagcgcg taatgccgcc
      661 gaagatgttg accagcagcg acttcacctt cttgtcgctg aagatcaggt cgatgccggc
      721 ggccatcgtc tcggcgttcg cgccgccgcc gatgtcgagg aagttcgcgg gcgtcccgcc
      781 cgcttcggcc accacgtcca gcgtgctcat cgtcaggccc gcgccgttgc cgatgatgcc
      841 gacctttccg tccagcttca cgtagttcaa gcccttctcg gccgcgatct tctcttgctt
      901 ggaggccggt ccgacgtcct tcaatttctc ggtccacgcc tgccggtaga tcgcgttgtc
      961 gtcgatcgag accttcgcgt ccagcgcaat gacgcttccg tcgcccatct ggaccagcgg
     1021 gttgacctcg accagcgacg cgtcagatgc gacgaacgca tcgaacagct tctgcagcag
     1081 cgccatcacg ttcttctgcg catcggccgg aatgccgccg ccgtagacga gccggcgaag
     1141 gtgatagggc tgcaggccgg tcagcgcgct gatgggaacc ttcgcgatcg cgtcgggttc
     1201 ggtcgcggcg acctcttcga tgtccacgcc gcctttgctc gagaccatgc cgaggaaccc
     1261 tttgccggct cggtcgagca agaaggaggc atagaactcg ttcttgatgt cgccggcgcg
     1321 ttcgaccagc acgcgcttga ccttgtggcc tttgatgtcc aagccgatga tctgctcggc
     1381 gaccgagacc gcctcgtcgg gggtcttcgc cagcttgatg ccaccggcct tgccgcggcc
     1441 gccgacctgc acctgcgcct tgaccacgac cgtgccgccg agcttctcgg cgatcgcgcg
     1501 cacctgcttg ggcgacgagg cgaccttgcc gtcaggcacc ggaacaccgt gcttcgagaa
     1561 cagctctttt ccttgatatt cgaacagatc catgcgccgc tcctgtgtgg ggcggcccgc
     1621 gcggtcgcga gcccgcgaaa ctctagcaac cggccgagtc gccgctcgaa tcggccggtt
     1681 tcgctcgctg ttcccggacg ggacttccgc ccggaaatcg gccagattcg ctgcggaaag
     1741 ggagtgatac catcggactc atgccgggca ccacggggct tacctaccag gctttgctcg
     1801 cgttccccga cgacggcctg cggcgcgaac ttctcgacgg agagctcatc gtgagcgccg
     1861 cgccccgcct gcgccaccaa gtgattctcg ggcgcctgca cctgttgttc ggaaacttcg
     1921 tcgaagagca ccggatcggg cacgtctaca tggccccgac cgacgtgttg ctctccgacg
     1981 acaacgtcgt cgagcccgac ttgatcttcg tcgccgacgg ccgcagcgag atcctcaccg
     2041 agatgaacat gcagggcgtg ccgtcgctgc tgatcgaggt cgtgtccgat ccgcgcatcg
     2101 accgggttcg caaacgcgac gtgtatgcgc gcttcggcgt tcccgaatac tgggtcgtcg
     2161 atcccgacgc cgaccggatc gaggtgtact gccacagccc agacggctac ggcaaaccgg
     2221 tgattttcga gccgggcgag acgctggaat atgaaggcct gcccggcctg cagatcgatc
     2281 tcacacgcct gttccgccgc gattaggcgg tcgtcgcaag agaggcgttg atccagtcca
     2341 cgatcgcttg cgtgggcgtg cccggcgtga agatctcggc gatacccatt tctttcagca
     2401 ccgggatgtc ttccttgggg atgatgccgc cgccgaacac ggcgatatcg ttcgcgcccc
     2461 gttccttgag cagctcgacc acgcgaggga acagcgtcat gtgcgcgccc gaatggatcg
     2521 acaggccgat cgcgtcggcg tcctcttgga tggcggcttc gacgatcatc tcgggcgtct
     2581 ggtgcaggcc cgtgtagatg acctccatcc cggcgtcgcg caaggcgcgc gcgacgacct
     2641 tggcgcctcg atcgtggccg tcgaggcccg gcttggcgat gacgacgcga accggcacgt
     2701 gatcgtcctt tctgcccgtt gatacagtgc gcgggcggct gtgtgcccga tcatagagag
     2761 tcggtgacgg ccgccgcaaa agaaacggct cggatgcgcg aagcgaagcc ctggctcgat
     2821 caggttctcg accccggatg ggtcgagcgc gacgccgacc tcgcctccgc gaacccgctc
     2881 gtttttcccg gatacgagcc cggtcgcgaa acggtccttg ccgcgtccgg cgccttggcg
     2941 ggccatgcgg tcgaggcgat ctcgttcgat ttctccgtgc tcggcggctc gatgggaatc
     3001 gtggccggcg aaaaggtcgc gcgcgcgttc gagcgcgcgc ccggccgccg cgcctcggtc
     3061 gtcgcgctgt gtgcgagcgg cggcgcccgg atgcaggagg gaatgaccgc gctggtgcag
     3121 atggccaaga cggtggtcgc acgcgagacg ctcgcacgcg cagggcttcc cttcgtcgcc
     3181 tacctgcgaa acccgaccac cggcggcgtc tacgcgtcgt tcgcctcgct cgccgacgtc
     3241 gtgtgggccg agccggacgc gaccgtgggc ttcgccggtc cgcgggtggc cgagaccgtc
     3301 acggggcggc cgctgccgcc cggctcgcac acggccggct tcgcggcacg gcacgggctg
     3361 atcgacggcg agatcgagcc gggagagctt cgggcccggc ttcgggcgct gctggatctc
     3421 gtgtccggcc gcgatcacac cgggccgatc gggaccatcg acgaaccggc cgcgcctgcc
     3481 gagcccgatg cgtggcagca ggtcatcttc gcgcgcgatc gcgcccggcc cgacggcgcg
     3541 gccttcgctc gtcgcgtgtg cgcggatctg atcgagattc acggcgaccg ccgcggaggc
     3601 ggcgacgacg acggcgtgct gtgcggagtc gcccggatcg gaggtgtgcg atgcggcgtg
     3661 gtcgcgctcg cgcgaacgca tcccgctgcc gccgggttcc ggaaggcaca gcggctgatc
     3721 gcgctcgcgg gacgctggcg cctgccggtc gtgacgttcg tcgacacgcc gggcgccgac
     3781 ccgtccagcg agtccgaagc cgccggcatc gcgcgcgcca tcgccgagac gttcgccgcg
     3841 gtcttgaatc acccgctgcc ggtggtgtcg gccgtcaccg gcgagggcgg cagcggcggc
     3901 gcgctcgcgc tcgcgtgcgc ggaccggctg ctgatcatgg agcacgcgat cttctcgatc
     3961 atcggtcccg aggccgccgc agcgatcctc aagcgcgacg atcccgcatc ggtcgcggca
     4021 gaccttcgcc tcacgtcgtt cgacctgcgg cggctcggca tcgccgacca gatcgtgccc
     4081 gagcccgcgc cggccgcgca cagcgatccg gatcgggccg ccgacacgct gggacgggcg
     4141 ctggcgctcg cgctcgcgga gatcgatccc gagcccgcac gcgagtcccg cacgcgacgc
     4201 tggcgagagg cgggcagcgc gttcctccga tgaggcggcg ataatgaagc cgtcatggag
     4261 ccgaagccct acaaaaagaa gcgggtgcat ccgccgcgca agatcgtccc tcgcggccgc
     4321 atggggatcc aatggatcat cggaccggtg atcatcgcag gcgtcatcct gatcgccggc
     4381 acgatctttt tgatcgtgaa gccgcaatag tcgcttttcg gatctgccat accgtccggc
     4441 cgcatgttca tccacagcct cgtgcgtttg cgcggtttat gcgtcgcaag caaggggtaa
     4501 ccctcaaccg ccccgcctcg ttgaccgagg tgagcaggga agacagaatt ggccgcgcgg
     4561 acgacctccc cggccgcagg aacccttccc cgtcaccata cgtcgacgac aaccacccga
     4621 tatcgggccg gaaggcgaag tgatggatcg gaaccagctg agcgagagct accggaaggc
     4681 gcaggctgct cgccgcgccg tgacccgggc gcttcgccgc ccccgcacct accgcagcgc
     4741 ggtcaaggag atcgcctggg cgggactgaa tctcgccatg tacccggccg gcgtggtgag
     4801 cgaggccctc gagccgaacc aagaccgcgg actcgcagga cggttcacgc ccgagcttcc
     4861 gctgcgatat cttgatcccg aagcggcgtc gacgccgatc atcttgctgc acggctactt
     4921 ccacaatcgt tcggcgttcc tggtcgcgcg ccgcgccctc cggcgcgcgg gcttccggca
     4981 tatctcgacg atgaactaca acgtcatcgg ccacgacgtc gaagagctcg cgcaacagct
     5041 gtccgcgcac gtggatcgag tgctcgagga gacgggcgcg catcgggttc acctgatcgg
     5101 tcattcactc ggcggcctcg tcgcacgcgc ctacatccag ctgtgcggcg gacgcgagaa
     5161 g
//
LOCUS       DATLFO010000072         6520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_130710, whole genome shotgun sequence.
ACCESSION   DATLFO010000072 DATLFO010000000
VERSION     DATLFO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130710"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2149)
                     /locus_tag="VM841_02455"
     CDS             complement(170..2149)
                     /locus_tag="VM841_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HVL89076.1"
                     /translation="MRRISIVFALAALLLSAAPIAAAEGAPLQEPVLERFALPLEDAG
                     AVASVLRPSRTFTRFQVAVPHRWTHARFDTTVRWTASNLVGEGSALTVEVDDVPVTAV
                     PVAAGPGSVTIASPLLPLDEHTITVTIRATLQAVPGVSARSAARGLFLRLDPFGRVQV
                     SGTGVAQPLLSDLPGALVERVGDRVTPLTIVFPPHPPAQAIHAAAIVAGAVSTATGFP
                     GVRIATLIDPGEAEIAARPGALVRFVDQPGDPVLSIARRSGGESALTVAGRGDALVRA
                     AWTAAYHAARLTGPTAVVRAATVPVPALAPEPRRIELGPAGVEDSEPVTLRLPFRLPV
                     NRVIEAAEVVLEVSYDAPAGARISVRANGKVMEARALRANESRTRVAVSLTDAVRPGD
                     NSIEIVLTPSARPDAALPARLDVLTGSTLALRTRERAEPSLGWWPFLGSGDRDWSRTV
                     VSLPASPTAQEVDAVVAVLSEAVRWSGGPLRYSIAFDASIQETLRTRNLVSLVRDPVH
                     VSRFVPGGATHGTLAVTRRGERAALVAVGARALAPLASSYFAGVVHGRAVVVDARGDA
                     RPIVPALGEPDSGRSWWLPAGVAAGLTFLVLLIGFIRAKRRFSPGQNDVPWNSSVPEA
                     VVPRTKSGVIPPVEPPVDTERALEDWLRIREEHRL"
     gene            complement(2142..3581)
                     /locus_tag="VM841_02460"
     CDS             complement(2142..3581)
                     /locus_tag="VM841_02460"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL89077.1"
                     /translation="MAASKARSQLPAPLYVAAAYVFFLGVPTLLCVVIGLSRPGVLTF
                     LHYAVATAYAVTSLMMLLEARTAIPRRKAGRTHTETLPTMPLTTVIVSAYLPNEQDLI
                     AETILRLTTQMHVPSYKLQIILAYNSPDDLPVEAELASFGQLNASFMPLRVVGSRSKA
                     QNILAALPYARGEMTVLLDADHHPHPDAFERAYRWLEAGYDVVQGRCVVRNQKENLLT
                     RLVSIEFEQIYGISHTGRSLAMDTAIFGGTNGYWRTSVLQEIGMDERMLTEDIDSSVR
                     AMLAGHRIIHDRSVISTELATTTLRSWWTQRLRWAQGWFQVTLRHQAAIWRSEHLNRR
                     TKLYWTYMLTWRELFPLLSLQIFALLIAALVLRIPVDWVRNWYLAGTTVITLSSGPLV
                     AIATYRIALWRTKKSLRPWFFIFAVTSLFYTTLKNMVAMVAMLREFRGESPWVVTTRQ
                     SAESKAKVKANEKAAARATRRPERVQLDA"
     gene            complement(3588..4028)
                     /locus_tag="VM841_02465"
     CDS             complement(3588..4028)
                     /locus_tag="VM841_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL89078.1"
                     /translation="MDLQDELRRHDSRDSTGSVLLCGTGQFESEMLRRALQRASFRVV
                     YAHDVDAAVLAVLGGTVDAVIVGDSMAPAPRVALIRWIRGSEGIERTPIVFLAEKGDP
                     ASAVRAYDAGVDMVVEKPVDVDLLGRKLGAVIRRAGPRRRAGGA"
     gene            complement(4182..5381)
                     /locus_tag="VM841_02470"
     CDS             complement(4182..5381)
                     /locus_tag="VM841_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL89079.1"
                     /translation="MTVIDRAKTPAAEETAGGIVVEDLVVEFVTDGYRIRALDALSFV
                     APPGQIVALLGPSGSGKTTLLSCLAGILTPASGRIVVNGRTIGSLSAAAMSEYRRTQV
                     GIVFQAFNLIPSLSARENVAAPLLAAGTRSRTALHRADELLAIVGMGERAHHKPGQLS
                     GGQQQRVAIARALAHDPPVLLADEPTANLDYVQAEAIVALLRDLRSQGRTILISTHDQ
                     RIVPIADAVVQMVPELEGKTGAGRVEYVAGESIFEQGTRGTLAYVIEDGQVDIIRILA
                     DGGQKHLATLGPRQYFGELAPLIGFPRSASARAKTNVVLRGYPIQEFRALMLRPGDGD
                     TDGGKTGEKPAKPAAARRRTPAERAAKKKSASVRRVPAASRTAARTPAAKKGSPAKRS
                     STTRKRS"
     gene            complement(5378..6415)
                     /locus_tag="VM841_02475"
     CDS             complement(5378..6415)
                     /locus_tag="VM841_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89080.1"
                     /translation="MTLRDLQFRLRRLVVVVIGTGVVFALLLLMTGLAQQFMREPQTT
                     VDGFGASRWIVRAGSTGAFTSAATIDESIAAELTGTPRADPVITARHSLILEGETVDI
                     VVIGIVPGGLGEPRVRSGAKANGRGRVVADDSAGVAVGERIRIGPQEFTVSGLTTDES
                     LFAGMPLIFMSLDDAQDLLYKGASNVTAVLTDRSPTSLPEGVAALTPAQIVEDARRPL
                     ENAISSIDLIRMLLWGVSALIIGGMVFLSAMERRRDFAVMKAAGASTRWLLGGVAIQG
                     ALIALASAVLAIGLQALLAPVFPMKVRITGATMVQLPVVAVIVALLAGGGGLRQVARI
                     DPAAAFGGPGA"
BASE COUNT         1082 a         2248 c         2241 g          949 t
ORIGIN      
        1 gcgggtcaag ttattcggca cggtcgcaga tcacccctgg ggctttgctc gctcaattgt
       61 tccggcagtt tacccatgtg ccgacgacgc ccggacattt cgaatcgatt aagcagcctt
      121 cacgatgggg tggaatcagg acaaatacga acgccgcccg ttcggcccgt tacagccggt
      181 gctcttctcg aatccggagc cagtcctcga gcgcgcgttc ggtgtccacc ggcggctcca
      241 ccggcgggat gacgccgctc ttggtccgcg gaacgaccgc ttccggaacg ctcgaattcc
      301 aggggacgtc gttctggccc ggcgagaagc gccgcttggc gcgaatgaat ccgatcagca
      361 gcaccaggaa ggtcagcccg gcggcgacgc cggcgggcag ccaccagctg cggcctgagt
      421 cgggctcgcc caaggccggc acgatcggcc gcgcgtcgcc gcgggcatcg acgaccacgg
      481 cccgtccgtg cacgacgccc gcgaagtagg aggaagcaag cggagcgagc gcgcgcgcgc
      541 cgacggcgac gagcgcggct cgctcgcccc gccgggtcac cgcgagggtt ccgtgcgtcg
      601 ccccgccggg cacgaaccgc gagacgtgga ccggatcccg aaccaacgac accaggtttc
      661 tcgtacgcag cgtctcttgg atggaggcgt cgaaggcaat cgaataacgc agcggaccgc
      721 cgctccagcg aaccgcttcc gacagcaccg caacgaccgc atcgacctcc tgggccgtgg
      781 gcgatgcggg aagcgacacg accgtgcgtg accagtcgcg gtcccccgat ccgaggaacg
      841 gccaccagcc gagcgacggc tcggcccgct cgcgcgtgcg cagcgccaac gtcgagccgg
      901 tgagcacgtc caggcgagcc gggagcgcgg cgtccggccg ggcgctcggc gtgagcacga
      961 tctcgatcga gttgtcaccg gggcgaacgg catcggtcag cgagaccgcg acgcgcgtcc
     1021 ggctctcatt ggcgcgcagc gctcgcgcct ccatcacctt cccgttcgcc cgaaccgaga
     1081 tgcgcgcacc ggccggcgca tcgtaggaca cttcgagcac gacctcggcc gcttcgatca
     1141 cgcggttgac ggggaggcgg aacggaagcc gcagagtcac gggctcgctg tcctcgacgc
     1201 ccgcgggacc gagctcgatc ctgcgcggct cgggagctag ggcggggacg ggcacggtcg
     1261 cggcacgcac gaccgcggtg ggtccggtca ggcgcgcggc gtggtaggcg gcggtccagg
     1321 cggcgcgaac cagcgcgtcg ccgcgtcccg caaccgtgag cgcggactcg ccgcctgaac
     1381 ggcgggcgat ggacaggacc gggtctccgg gctgatccac gaaacgtacg agcgcgccgg
     1441 gcctcgcggc gatctcggcc tcaccgggat cgatcagcgt cgcgatccgc acgccgggaa
     1501 agccggtcgc ggtcgagacg gcgcctgcga cgatcgcggc agcatgaatg gcctgtgcgg
     1561 gcgggtgggg cggaaagacg atcgtgagcg gcgtgacgcg gtcgcccacc cgctccacca
     1621 gcgcgcccgg cagatccgaa agcagcggct gcgccacgcc cgtgcccgac acctggaccc
     1681 gtccgaaggg atcgagccgc aggaacagtc ctcgcgcagc cgaccgggcc gacactcccg
     1741 gcaccgcctg cagcgtcgcg cggatcgtga ccgtgatcgt gtgctcgtcg aggggaagaa
     1801 gcggtgaggc gatcgtcacc gagcccggtc cggcggccac ggggaccgcg gtcacgggca
     1861 cgtcgtcgac ctcgacggtg agcgcggatc cttcgccgac gaggttcgac gcagtccatc
     1921 gcacggtggt atcgaatcgc gcgtgcgtcc accggtgcgg gaccgcgacc tggaaccgcg
     1981 tgaacgtgcg cgacgggcga aggacgctcg caaccgcgcc cgcatcctcg agcggcaacg
     2041 caaaacgttc gaggacgggc tcctgcaaag gagcgccttc cgccgccgcg atcggcgcgg
     2101 ccgaaagcag cagcgccgcg agagcgaaca cgatcgagat cctacgcatc cagctgcacc
     2161 ctctccggcc ggcgcgtcgc acgcgctgcc gccttctcgt ttgccttgac cttcgccttc
     2221 gactcggcgg attgtcgcgt cgtgaccacc cacgggctct cgccgcggaa ctcacgcagc
     2281 atcgccacca tcgcgaccat gttcttgagc gtcgtgtaga acaggctcgt cactgcgaag
     2341 atgaagaacc agggccgcag gctcttcttc gtccgccata gcgcgatgcg gtaggtcgcg
     2401 atcgccacga gcggtccgga cgacagcgtg atcaccgtgg tgccggccag ataccagttg
     2461 cgcacccagt cgacggggat ccgcaggacc agggcggcga tcagcagcgc gaaaatctgg
     2521 agcgacagca gcgggaacag ctcacgccag gtgagcatgt acgtccagta cagcttcgtc
     2581 cgcctgttga gatgctcgct gcgccagatc gcggcctgat gacgcaaagt cacctggaac
     2641 catccttgcg cccaccggag ccgctgcgtc caccacgacc gaagcgtcgt ggtcgcgagc
     2701 tcggtcgaga tgacggaccg gtcgtggatg atccggtggc cggcgagcat ggcccgcacg
     2761 ctggagtcga tgtcctcggt gagcatccgc tcgtccatgc cgatctcctg aagcaccgag
     2821 gtccgccaat agccgttggt tccgccgaag atcgccgtgt ccatcgcgag cgagcgtccg
     2881 gtgtgtgaga tgccgtagat ctgctcgaac tcaatgctca ccaggcgagt gagcaggttc
     2941 tccttctggt tccggacgac gcatcggccc tgcaccacgt cgtagcccgc ctcgagccag
     3001 cgataggcgc gctcgaaggc gtccgggtgc ggatgatgat cggcgtcgag caggaccgtc
     3061 atctcaccac gggcgtacgg cagggcggca aggatgttct gagcttttga ccggctgccg
     3121 acgactcgca acggcatgaa cgaggcgttc agctgaccga acgacgcgag ctcggcttcg
     3181 acgggcagat cgtcggggct gttgtaggca agaatgatct ggagcttgta gctcggcacg
     3241 tgcatctgcg tggtcagacg caagatggtc tcggcgatca ggtcctgctc gttgggaagg
     3301 taggccgaca cgatcaccgt cgtgagcggc atcgtcggaa gcgtctcggt gtgcgtgcgg
     3361 ccggcctttc ggcgcgggat cgcggttcgg gcctcgagca gcatcatcag cgacgtcacg
     3421 gcgtaggcag tcgccaccgc atagtgcaag aaggtaagaa cgccgggacg gctcaagccg
     3481 atgacgacgc acagcagggt cggaacgccg aggaagaaga cgtaggccgc cgcaacgtag
     3541 agcggggcgg gaagctggga tctggccttg gaggctgcca cggagaccta cgctccgccg
     3601 gcccttcgcc gcgggcctgc gcgacggata accgctccga gcttgcgccc gagcaggtcc
     3661 acgtcgacgg gcttttccac gaccatgtcc acacccgcgt cgtaggcacg cacggcgctc
     3721 gcgggatcgc ctttttcggc gaggaacacg atgggagtgc gctcgatgcc ttcgctgcct
     3781 cgaatccacc ggatcagcgc caccctcggc gcgggagcca tcgagtcgcc cacgatcacc
     3841 gcgtccacgg tcccgccgag cacggccagg acggccgcat ccacgtcatg ggcgtagacc
     3901 acccggaacg aggctcgttg cagggcacga cgaagcatct cgctttcgaa ctggccggtg
     3961 ccgcacagca gcacggagcc ggtagagtcg cgcgagtcgt gccgtcgaag ctcgtcttga
     4021 agatccatgt tgtcctcgat gtccgcagcc gtggattctc cttctcattc ggcatcgccg
     4081 gggggctcgc ttgagatagg gcaggaccgc cagaacgtgt ccgcgcacaa aaagttcctt
     4141 aaaccggcag ccacgggact gcctcagccc ggaatgaccg tttaggagcg cttgcgcgtg
     4201 gtgctggatc gcttcgccgg ggaacccttc ttggccgcgg gcgtgcgagc cgccgtacgg
     4261 ctcgcagcgg gaacccggcg aaccgatgcg ctcttcttct tggcagcgcg ctcggcggga
     4321 gtgcggcggc gagccgccgc cggcttggcc ggcttctcgc cggtctttcc gccgtccgtg
     4381 tcgccgtccc ccggccgcag catcaacgcc cggaactcct ggatcgggta tccgcgaagc
     4441 accacgttgg tcttcgcccg tgccgaggcc gaccgcggga agccgatcag cggcgcgagc
     4501 tcgccgaagt attgcctcgg accgagcgtt gccagatgct tctggccgcc gtcggccagg
     4561 atccggatga tgtccacctg gccgtcttcg atcacgtagg cgagggtgcc gcgcgttcct
     4621 tgctcgaaga tcgattcgcc ggcgacgtac tccacacgtc cggcaccggt cttgccttcg
     4681 agctcgggca ccatctgcac caccgcgtcg gcgatcggca cgatgcgctg gtcgtgggtc
     4741 gagatcaaga tggtgcgccc ttgggagcga aggtcgcgca acagcgcgac gatggcctcg
     4801 gcttgcacat agtccaggtt cgcggtcggc tcgtcggcga gcagaaccgg cgggtcgtgc
     4861 gcgagcgctc gcgcgatcgc gacgcgctgc tgctggccac ccgagagctg gcccggcttg
     4921 tgatgggcgc gctcgcccat gccgacgatc gccagaagct cgtccgcccg gtgcagcgcg
     4981 gtccgcgagc gggtgccggc ggcgagcagc ggcgccgcca cgttctcgcg ggcgctcagc
     5041 gacgggatca agttgaatgc ctggaacacg atgccgacct gcgtgcggcg gtactcgctc
     5101 atcgccgcgg ccgacagcga gccgatcgtg cgtccgttga cgacgatccg gcccgaggcc
     5161 ggggtcagga ttcccgcgag gcacgacagc agcgtcgtct tgcccgagcc cgacgggccg
     5221 agaagagcca cgatctggcc gggaggggcg acgaacgaaa gcgcgtcgag cgcgcgaatg
     5281 cggtagccgt cggtgacgaa ctcgacgacg agatcctcga cgacgatgcc gccggcggtc
     5341 tcttcggccg ccggagtctt tgcgcgatcg atgacggtca tgctcccggc cctccgaatg
     5401 cggccgcggg atcaatccgc gcgacctggc ggagcccgcc gcctccggcg agcaacgcga
     5461 cgatgacggc gacgaccggc agctggacca tggttgcccc ggtgatgcga accttcatcg
     5521 ggaacacggg cgcgagcagc gcctgcagcc cgatcgccag caccgccgac gcgagcgcga
     5581 tcagcgcgcc ctggatggcc acaccgccga gcagccagcg cgtcgaggcg cccgctgcct
     5641 tcatgaccgc gaagtcccgg cgccgttcca tcgccgacag gaacaccatc ccgccgatga
     5701 tcagcgcgga cacgccccac agcagcatgc ggatcagatc gatcgagctg atcgcgttct
     5761 cgagcggccg acgcgcgtcc tcgacgatct gggccggcgt cagggccgcg actccctcag
     5821 gcaggctggt cggcgagcgg tcggtgagca ccgcggtcac gttcgacgct cccttgtaga
     5881 gcagatcctg cgcgtcgtcc aggctcatga agatgagcgg catgccggcg aacaaggact
     5941 cgtcggtggt caggccggac acggtgaact cttgcggtcc gatccggatc cgctcgccga
     6001 ccgccacgcc cgccgagtcg tcggcgacca cccgtccgcg gccgttcgcc ttcgcacccg
     6061 agcgcacgcg cggctcgccg agcccgccgg gcacgatgcc gatcaccacg atgtccaccg
     6121 tctcgccttc gaggatgagg ctgtggcgcg cggtgatgac gggatcggcc cgcggcgttc
     6181 cggtgagctc ggccgcgatc gactcgtcga tcgtcgccgc ggaggtgaag gcgcccgtcg
     6241 atccggcgcg cacgatccag cgcgacgcgc cgaagccgtc caccgtcgtc tgcggctcgc
     6301 gcatgaactg ctgcgccagc ccggtcatga gcagcagcag cgcgaacacg acgccggtgc
     6361 cgatcacgac caccaccaga cggcgcagcc ggaactgaag atcgcgaagc gtcatcagga
     6421 gcacaggatc atcctttcgc tcgcgctgcg gtcagcgcgc gaatcgtgtc ggtggcgccg
     6481 gcctcgatcc agtcgcggat cttcggaagc gcctcatcgt
//
LOCUS       DATLFO010000073         8152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_71190, whole genome shotgun sequence.
ACCESSION   DATLFO010000073 DATLFO010000000
VERSION     DATLFO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71190"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VM841_02480"
     CDS             <1..270
                     /locus_tag="VM841_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HVL89081.1"
                     /translation="ESLTLIQTGKVMNFPVALVGTDYWTGLIDWMKARTLEQGMISQE
                     DLDIFYLCDDPESVVPYLRAVMQRRVEEHTPKEEPVVQPHKAGAE"
     gene            267..617
                     /locus_tag="VM841_02485"
     CDS             267..617
                     /locus_tag="VM841_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVL89082.1"
                     /translation="MSDEIEVCRSEDLPPGSVTGAGRWAVGNAAGERFAVTRRCRHMV
                     ADLAKGSIDADGCLVCPRHGAAYDVESGRMTRGPRGIYAKVPGLEAGLKAATKALPLG
                     RGSVTERDGVLFVE"
     gene            679..1107
                     /locus_tag="VM841_02490"
     CDS             679..1107
                     /locus_tag="VM841_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89083.1"
                     /translation="MKRALLAVFAVMAAFTVAGAHRTTVVVGAGVLSTGELFEVAAFD
                     AGDRDGGVLRYRAGPDAAEAVISVDCVSVSWSLVPLSLSHVIALSGEDQDGKRHHAVL
                     MDVGPGTLAADHARFMPAGQDACGVTFGPLRRVDGFVDAT"
     gene            complement(1319..1564)
                     /locus_tag="VM841_02495"
     CDS             complement(1319..1564)
                     /locus_tag="VM841_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89084.1"
                     /translation="MSRAPHPRRSECPDDDAFVYTIAFEDAGALSVWAYRDRDAAWRR
                     FHALRRKATKAGSQAQILFSFTHVDCDAPQRHVYEED"
     gene            1830..2480
                     /locus_tag="VM841_02500"
     CDS             1830..2480
                     /locus_tag="VM841_02500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVL89085.1"
                     /translation="MVSFPSNGSTANGYLAGDGPGIVVIQEWWGLNDQIRGVCDDFAG
                     EGFTALAPDFYHGKVTAEPDEAGKLMMALDIDRTAKAARGAAAFLAGQTGGRVGVIGF
                     CMGGQLALLAGTVAPEHVGAVVDMYGIHPAVKPDYAKMDAPVLGLFAEHDDFITEEVR
                     AGLSAELTAAGVEHDFHVSPGTHHAFTNETNPDVYDRDASQDARRRVLEFFRANLG"
     gene            complement(2532..3647)
                     /locus_tag="VM841_02505"
     CDS             complement(2532..3647)
                     /locus_tag="VM841_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVL89086.1"
                     /translation="MSGLRFRLAGIPVRVEPVFFLIIVLIGGLDRPGILILTFAATAF
                     VSILFHEFAHAFAFRAFGRSPSIVLHAFGGLTGAPGAPLSRARDVLVSAVGPLSQIIV
                     LGIPALMLRLPVARAFRSVNWYIALTDAAWINIGWGILNLMPVLPLDGGRIMAGLLGG
                     RRHGLRIAHAIGVVVAAAGAAWGLANQRIFLALFGAMFVAINVAGFRDQRNAPHIARV
                     REGHARLDAGDAAGAAHAARAVLDDPVAAARVRTEAAELAAWAMLSRGEREAAAEVLA
                     RLPQGTERSAYLAAFTAIERGDHDAGLVVAARAFAAGDGPPPNHLLAMRLSREGLVER
                     LAAILRGADDEGRALGELEQALREAGLHEEASRVAGL"
     gene            complement(3687..5069)
                     /locus_tag="VM841_02510"
     CDS             complement(3687..5069)
                     /locus_tag="VM841_02510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL89087.1"
                     /translation="MTDVTGALDALSAKLEGSLTRPGDPRYEELRAVWNAMIDRRPAA
                     IVRAATIGDVVAVVNAAREHGVLLAVRNGGHSMPGFSTCDDGIVLDLRDLNTVTFDPE
                     AGRVAVQGGAMILDLDTATQAHGHAVPMGTVTHTGVAGLTLGGGLGRLTRMHGLSIDA
                     LTGVQVVTADGEIVEASEDENADLFWGMRGAGPNFGVATRLDFATFPIGTDMGAAMAV
                     YDLDHARDVLIALGEYERDAPEHGIVIAALKTLPAQPPFPPEIHGRTVLFVGANNFGP
                     PDTADKELREIARFAPTLFDLALRMPFLAAQQMNDQYFGWGQRNYVKGGLLETITPAM
                     ADAAVEALEGLPDDHAEIDFIMMGGASARVAEDATAYSGRDARFFNGIQSIWHDAAAD
                     DAHRGWVRSTFDALSRFHMSANYVNALEESSENEVARAYGAGKYERLVELKRRYDPGN
                     LFRLNNNIRP"
     gene            complement(5260..5511)
                     /locus_tag="VM841_02515"
     CDS             complement(5260..5511)
                     /locus_tag="VM841_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89088.1"
                     /translation="MAFVVVMTMALVTALALIPCAIVLLIKSGGGARARRQREAATRA
                     RMGAIFGYDTIPVPAATTTTVRAMRSAVRRDGVLVASWM"
     gene            complement(5662..6231)
                     /locus_tag="VM841_02520"
     CDS             complement(5662..6231)
                     /locus_tag="VM841_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVL89089.1"
                     /translation="MSTDAISILKQDHKEVEQLFRKFEQAGDRAHKSKRSLMDRIVRE
                     LSVHAAIEELIMYPAARETLDDDDPVLEALEEHHIVKWTLSELERMDPTHARFDAKAK
                     VLFEMVRHHVEEEESELFPALRDKLGRTRLSEIGEALMQAKKAAPTRPHPRMPDQPPG
                     NVLGGMAMAAGDRVIDLTRDIKEKVSGKR"
     gene            complement(6351..7880)
                     /locus_tag="VM841_02525"
     CDS             complement(6351..7880)
                     /locus_tag="VM841_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89090.1"
                     /translation="MSRRSAVVAAIAVLAAALSLVPASAVSVDPASLPYDPAHVLVRF
                     DAGTGSAERAEAHRLIGSHVVNRFASLGIDVVALPDGLAAPDAVARYARDARVEHASL
                     NKKLFKLSTPGDTLFGQQWGFHNTAQSVSGSLVRGVADADIDAPEGWTAAYGAGSFPT
                     SGGTLVAVLDTGIDLGHTDLMGKVRHCAGATSAVGVVVEGACQDDNLHGTHTAGTVAA
                     NTNNTVGVAGTAPNAELAIFKFLNAAGVGFLADEIAGLQWATARGAKVFSMSYGSYDP
                     DSNEEKALQNAYNAGVLPIAAAGNDYDSTKNYPAFYNVVMSVASHNQADAISDFSNCN
                     GDVEIAAPGEDVWSTLPGNSYGPLSGTSMATPHVAGVAALLMWKKGTSNVQTRNLMVN
                     SAEAVASTGGRSACNGIKRVNLASALGSGDTTPPAPGGIAGTVTDQQAKTPLGGATVD
                     CGTGGTATTASNGTYSLSNVPAGSYSCTASKSGYRSKTQSVGVSSGQTAVADFALRAS
                     R"
BASE COUNT         1217 a         2752 c         2895 g         1288 t
ORIGIN      
        1 gagtcgctca ccctgatcca gaccggcaag gtcatgaact ttcccgtcgc gctcgtcgga
       61 accgactatt ggaccggcct gatcgactgg atgaaggcgc gcacgctcga gcagggaatg
      121 atctcgcaag aggatcttga catcttctat ctctgcgacg atcccgagtc cgtcgttccc
      181 tatcttcgcg cggtgatgca gcgacgcgtc gaggagcaca cccccaagga ggaacccgtg
      241 gttcagcccc acaaggcagg cgccgagtga gcgacgaaat cgaagtttgc cgcagcgagg
      301 acctgccgcc gggctcggtg acgggtgcgg gacggtgggc ggtcgggaac gccgcgggcg
      361 agcgcttcgc ggtaacgcgc cgctgccgcc atatggtcgc cgacctggcg aaggggagca
      421 tcgacgcgga cggatgcctg gtgtgtccgc gccacggcgc ggcctacgac gtcgagtcgg
      481 gtcgcatgac ccgaggccca cgaggcatct acgcgaaggt tcccggcctc gaggcgggcc
      541 tgaaggcagc caccaaggcc cttccgctcg gccgcggatc cgtcaccgag cgagatggcg
      601 ttttgttcgt cgagtagcag gattccgctt cggcccccgc gaaatctccg cccgacagcc
      661 ggaaccaggg ggcatctcat gaagcgtgcg ttgcttgccg tcttcgccgt catggccgcg
      721 ttcacggtcg cgggagcgca tcgaaccacc gtcgttgtcg gcgcgggagt actttcgacg
      781 ggcgagttgt tcgaggtcgc cgcattcgac gcgggagacc gtgacggtgg cgtgctgcgc
      841 taccgcgcgg gcccggatgc ggctgaggcc gtgatctcgg tcgattgcgt ttcggtcagc
      901 tggtcgctcg tgccgttgag cctcagccac gtgatcgcgc tcagcggcga ggatcaggac
      961 gggaagcggc atcacgcggt gctcatggac gtcggcccgg gaacgctcgc cgccgatcac
     1021 gcccggttca tgccggccgg gcaggacgcg tgtggcgtga cgttcggccc gctccgccgc
     1081 gtcgacggct tcgtcgacgc gacctgacac tcccgatacg aagcaacggc catcgcgata
     1141 agccaacgtt cgttccgaat gaatcgacga cggaggcctt cgacacggct ccttgcgaaa
     1201 ggccggtcgt agacttgcga cgtctttccg tgagaaggag cacgatgggt actcggttcg
     1261 acgtgtcgtc cgttccgttg cagggcggct atgtcgccaa gagacgcgac gcggccggtc
     1321 agtcctcttc gtagacgtga cgctgcggcg cgtcgcaatc gacgtgcgtg aagctgaaca
     1381 ggatctgcgc ctgcgagccg gccttggtcg ccttgcggcg taaggcatgg aaccggcgcc
     1441 aggccgcgtc gcggtcgcgg taggcccaca cgctcagcgc gccggcgtcc tcgaacgcga
     1501 tcgtgtagac gaacgcgtcg tcgtcgggac actcgctgcg gcggggatgc ggagcccggg
     1561 acacgggccg atgggggagg ctcttgttca tgactcgact gtcgcggacg gcgacgacaa
     1621 tgacagcgtt gtcatttcgg agcgggcgtc agcgccgcac gacatgaggg ccggcgccgg
     1681 cctagggggt tggctcttcg acctggccgg cgaccttgtc gtcgagcggt tcgagccgcc
     1741 cgtgggagaa cagctccgag gtgttggatc gaacttgccc ggcgccggta ggctgccgcg
     1801 ccgaaacagg agggatcgac gtgggcgaga tggtcagctt cccgagcaac ggctccaccg
     1861 cgaacggcta cctggcgggc gacgggcccg ggatcgtcgt gatccaggaa tggtgggggc
     1921 tcaacgatca gatcagaggc gtgtgcgacg acttcgcggg tgaaggcttc accgcgctcg
     1981 cgcccgactt ctatcacggg aaggtcaccg ccgagcccga cgaggccggc aagctgatga
     2041 tggcgctcga tatcgaccgc acggccaagg ccgcgcgggg cgccgctgcc ttcctcgccg
     2101 gccagaccgg cggccgggtc ggcgtgatcg gattctgcat gggcggacag ctggcgctgc
     2161 tcgccggcac ggtcgcgccc gagcacgtcg gcgccgtcgt ggacatgtac ggcatccatc
     2221 ccgcggtcaa gcccgactac gcgaagatgg acgcgcccgt cctcggcctg ttcgccgagc
     2281 acgacgactt catcaccgag gaggtccgtg cgggcttgtc ggccgagctg acggccgccg
     2341 gtgtcgagca cgacttccac gtctcccccg gcacgcatca tgccttcacg aacgagacca
     2401 atcccgatgt ctacgaccgg gacgcctcgc aggacgcgcg ccggcgcgtg ctggagttct
     2461 tccgcgcgaa tctcggctag aggggcgggt cgccctcgac gagatgccgg gatgcatcgc
     2521 cgcaaaaagc gtcagagtcc ggccacgcgc gaggcttcct cgtgcaggcc cgcctcgcgc
     2581 agcgcctgct caagctcgcc gagggcgcgt ccttcgtcgt ccgccccgcg caggatcgcg
     2641 gcgagccgct cgaccagccc ctcgcgcgag agccgcatcg cgagcaggtg gttcggcggc
     2701 gggccgtccc cggccgcgaa cgcgcgcgcc gcgacgacca gccccgcgtc gtgatcgccg
     2761 cgctcgatcg cggtgaaggc ggcgaggtag gccgatcgtt cggttccctg cggcagccgt
     2821 gcgagcacct cggccgcggc ctcgcgctcg ccgcgcgaca gcatcgccca agccgcgagc
     2881 tcggcggcct cggtccgcac gcgcgccgcc gcgaccggat cgtcgagcac cgcccgtgcc
     2941 gcatgcgcgg ctcccgcggc gtcgcccgcg tcgagccggg cgtggccttc ccggacgcgc
     3001 gcgatatgcg gcgcgttgcg ctggtcgcga aagcccgcca cgttgatcgc gacgaacatc
     3061 gcgccgaaca gcgccaggaa gatccgctgg ttcgccagac cccaggccgc tcctgcggcc
     3121 gcgaccacga cgccgatcgc gtgcgcgatc cgcaggccgt gccggcggcc gcccagcagg
     3181 ccggccatga tgcgtccgcc gtccagcggc agcaccggca tcaggttgag gatgccccat
     3241 ccgatgttga tccaggcggc gtcggtcagc gcgatgtacc agttgaccga ccggaacgcg
     3301 cgcgccaccg gcagccgcag catcagcgcc gggatgccga gcacgatgat ctggctcagc
     3361 ggccccaccg ccgacaccag gacgtcgcgc gcccgcgaca gcggcgcgcc gggtgcgccg
     3421 gtcaagccgc cgaacgcgtg cagcacgatg ctcggcgaac ggccgaaggc gcggaacgcg
     3481 aacgcgtgcg cgaactcgtg gaacaggatc gagacgaacg cggtcgcggc gaaggtcagg
     3541 atcaggatgc cgggccggtc cagccccccg atcagcacga tgatcaggaa gaagaccggc
     3601 tcgacccgaa ccgggatccc tgcgaggcga aagcgaaggc cggacacggc gccagcgtac
     3661 cggcgccgtg ccgctctcgc gcgccgctac ggacgaatgt tgttgttgag acggaacagg
     3721 ttgcccgggt cgtaccggcg tttgagctcg accagccgct cgtacttgcc ggccccgtag
     3781 gcgcgcgcca cctcgttctc gctcgattcc tcgagcgcgt tgacgtagtt cgcgctcatg
     3841 tggaagcgcg aaagggcgtc gaaggtcgag cggacccatc cgcgatgcgc atcgtcggcg
     3901 gccgcgtcgt gccagatcga ctggatcccg ttgaagaacc gggcatcgcg tccgctgtag
     3961 gcggtcgcgt cctcggccac acgcgcggac gcgccgccca tcatgatgaa atcgatctcg
     4021 gcgtgatcgt ctggcagccc ttcgagcgcc tcgacggcgg cgtcggccat ggcaggcgtg
     4081 atcgtttcga gcaggccgcc cttgacgtag ttgcgctgac cccagccgaa gtactggtcg
     4141 ttcatctgct gggcggccaa gaacggcatc cgcagcgcca ggtcgaacag ggtcggggcg
     4201 aagcgtgcga tctcgcgaag ctctttgtcg gccgtgtcgg gcgggccgaa gttgttcgcg
     4261 cccacgaaca ggaccgtgcg gccgtgaatc tcgggcggga acggcggctg cgcgggcagc
     4321 gtcttgagcg ctgcgatcac gatcccgtgc tcgggcgcgt cgcgctcgta ctcgccgagc
     4381 gcgatcagca cgtcgcgcgc gtggtcgaga tcgtagacgg ccatcgcggc gcccatgtcg
     4441 gtgccgatcg ggaacgtggc gaagtccagc cgtgtggcga cgccgaagtt cggtccggcg
     4501 ccgcgcatcc cccagaacag atcggcgttc tcgtcttcgg atgcctccac gatctcgccg
     4561 tcggcagtga cgacctgaac gcccgtgagc gcgtcgatgg acaggccgtg catccgggtg
     4621 agccgcccca ggccgccgcc gagcgtcagg cccgcgacac ccgtgtgcgt cacggtcccc
     4681 atcggaacgg cgtggccgtg cgcctgggtc gcggtatcca ggtccaggat catcgccccg
     4741 ccttggacgg cgacgcgacc cgcttcggga tcgaacgtca ccgtgttcag gtcgcgcaga
     4801 tccagcacga tgccgtcgtc gcaggtcgag aagccgggca tgctgtgccc gccgttgcgt
     4861 acggccaaga gcacgccgtg ctcgcgggcc gcgttcacca cggcgacgac gtcgccgatc
     4921 gtcgcggcgc ggacgatggc cgcgggcctg cggtcgatca tcgcgttcca gaccgcccgc
     4981 agctcctcgt agcgtgggtc gccgggccgg gtcaggctgc cttcgagctt cgccgagagc
     5041 gcgtcgagcg cgccggtcac gtcggtcacg ggaaccgccc ctttctcgtg tcgtctcgct
     5101 cgacggtagc ccacgacggg cccttttgaa aagtgccgag cgaggagcga cgatcgcgac
     5161 gggtccctgg gctgccgcga ccagggcgcc gggcctctct tgccgagcgc cggggcgctt
     5221 ggcgcatcgg gtcacggagg aggggtcacg cgagcgtcgt cacatccagg atgcgaccag
     5281 gacgccgtcg cggcgcacgg ccgatcgcat cgcgcgcacc gttgtcgtgg tcgccgcggg
     5341 aacggggatc gtgtcgtagc cgaagatcgc acccatccgt gcgcgggtcg cggcctcccg
     5401 ttgccgccgg gcgcgggcgc cgcctccgct tttgatcagg agcacgatcg cgcaggggat
     5461 gagcgcgagc gcggtcacca atgccatcgt catcacgacc acgaatgcca tgccgggcct
     5521 ccgatccggg tgggcgagct gccttcggat ctggatcgga gacccggcgg cgattcctcg
     5581 ccatccggcg ggatggtttc tctagtccgt tcggaacggt tcgataccgt ccgtgaggag
     5641 ggttgcgccg aacgggtgtg gtcagcgctt gcccgagacc ttctccttta tgtcgcgcgt
     5701 caggtcgatg acccggtcgc cggccgccat cgccatgccg ccgagaacgt tgcccggggg
     5761 ctggtccggc atacgcgggt gcggccgggt cggagcggcc ttcttggcct gcatcagcgc
     5821 ttcgccgatc tcgctcaggc gcgtccggcc gagcttgtcg cgaagggccg ggaacagctc
     5881 ggactcttct tcctcgacgt ggtgacggac catctcgaac aggactttgg ccttggcgtc
     5941 gaagcgtgca tgcgtgggat ccatgcgctc gagctcggac agcgtccact tcacgatgtg
     6001 atgctcctcg agggcctcca gcaccggatc gtcgtcgtcg agcgtctcgc gcgccgccgg
     6061 gtacatgatc agctcctcga tcgcggcgtg gaccgacaac tctcgcacga tgcgatccat
     6121 caacgagcgc ttgctcttgt gcgcgcggtc gccggcctgc tcgaactttc ggaacagctg
     6181 ctcgacctct ttgtgatctt gcttgaggat cgagatggcg tcggtggaca tgaaatcact
     6241 cctcgcgaaa tcgggggcct gcccttgagg tacccggtgt cgccggagcg gaaacgacgc
     6301 gaggcgcgga cgggggtcgc cgccggccgg cgacccccga acgcgcttgc tcagcggctc
     6361 gcgcgcaacg cgaagtcggc cacggcggtc tggccgctgc tcaccccgac gctttgcgtc
     6421 ttgcttcggt acccgctctt ggacgcggtg cacgagtagg agccggccgg aacgttgctc
     6481 agcgagtacg tgccgttact agcggtcgtg gccgttccgc cggtcccgca gtccaccgtc
     6541 gcgccgccca gcggggtttt ggcctgctgg tcggtcacgg tgcccgcgat gccacccggg
     6601 gccgggggag tggtatcgcc cgagccgagc gcgctcgcga ggttgacccg cttgatgccg
     6661 ttgcaggcgg atcgcccgcc ggtcgaggcg accgcctcgg ccgagttcac catcaggttg
     6721 cgggtctgga cgttgctcgt acccttcttc cacatgagaa gcgccgcgac gcccgcgacg
     6781 tggggggtcg ccatcgacgt tcccgacagc gggccgtagg agttgcccgg cagcgtggac
     6841 cacacgtctt cgcccggcgc ggcgatctcg acgtcgccgt tgcagttcga gaagtcgctg
     6901 atcgcgtcgg cctgattgtg cgaggccacc gacatcacca cgttgtagaa cgccggatag
     6961 ttcttggtgg agtcgtagtc gttgcccgcg gcagcgatgg gcaggacgcc cgcgttgtag
     7021 gcgttctgaa gggccttctc ctcgttggag tcggggtcgt aggagccgta ggacatgctg
     7081 aagaccttcg cgccgcgggc cgtggcccac tgcagcccgg cgatctcgtc ggcgaggaat
     7141 cccaccccgg cggcgttgag gaacttgaag atcgcgagct cggcgttcgg cgccgtgccc
     7201 gccacgccca cggtgttgtt ggtgttcgcg gcgacggtgc ctgccgtgtg ggtgccgtgc
     7261 aggttgtcgt cctggcaggc gccctcgacg acgaccccga ccgcgctcgt cgcgcctgcg
     7321 caatggcgca ccttgcccat caggtccgtg tgcccgagat cgattccggt gtcgagcacg
     7381 gccaccagcg ttccgccgga ggtcgggaac gagccagcgc cgtacgcggc ggtccagcct
     7441 tcgggcgcgt cgatgtcggc gtcggcgacg ccgcgcacga ggctgccgga caccgactgg
     7501 gcggtgttgt ggaatcccca ttgctgcccg aagagggtgt cgccgggcgt ggagagcttg
     7561 aagagcttct tgttgaggga cgcgtgctcg acgcgggcgt cgcgtgcgta gcgagcgacg
     7621 gcatcagggg cggcgagtcc gtcgggaagc gcgacgacgt cgatgccgag cgatgcgaat
     7681 cggttgacga cgtgtgaccc gatcaggcga tgcgcctccg cgcgctccgc agagccggtt
     7741 ccggcgtcga aacgcaccag cacgtgcgcc ggatcgtacg gaagcgatgc gggatcgacg
     7801 gatacggccg aggccgggac gagcgacagg gcggcggcca gaacggcgat ggccgccaca
     7861 acggcagagc gacgtgacat gaatcctcct cgcggacggg ggcgcgagcc cggtttcatc
     7921 gttgccggcc cagtgtcgca aaccgatcaa gcggttcgct agcaaccgat tggggacaaa
     7981 acggcccccg accaagcgga tcagcttgct tcggcgttca ggcgggctct ggtctgcggg
     8041 ccgaggtagc cggtcggccg gatcccgcgg gagcgctgat agcgccgcag cgcctccttc
     8101 gtctcgggcc cgaacgtgcc ggtgatgcgg ccgccgggat atgcccccgc at
//
LOCUS       DATLFO010000074        11047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_81001, whole genome shotgun sequence.
ACCESSION   DATLFO010000074 DATLFO010000000
VERSION     DATLFO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81001"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VM841_02530"
     CDS             complement(<1..822)
                     /locus_tag="VM841_02530"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HVL89091.1"
                     /translation="MRRDPRIVAAVVALLVAVGVAAFRITASGSNRPGRAGDTLLFRN
                     DPAPELVGLSQWFNAEPMTLKALRGKVVLIDFWTFSCVNCVRTFPEIRAFYDTYKNAG
                     LEVIGIHSPEFDFEKSPDNVRKAMRAHSVVWPVAMDNDHKTWEAFRNRFWPHVYLIDA
                     EGRIRYDRVGEGGAAEIERAIRALLDEAGVALPPPTNVTVKGPSGGITPEIYLGWLRG
                     EQQKFLGNESGFVRDRPHDYLVPLDPPAGGAGTAAYFLEGRWRATEQFVEAAAAVP"
     gene            complement(1016..1315)
                     /locus_tag="VM841_02535"
     CDS             complement(1016..1315)
                     /locus_tag="VM841_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVL89092.1"
                     /translation="MDTTVSWTEARCATGDASLLHLFFSEDETEISLAKQVCGDCPLR
                     LPCYEGAVRRREPWGVWGGELFDRGAPIARKPRRGRPPKRPVEVAAGANAEAEVA"
     gene            complement(2029..3567)
                     /locus_tag="VM841_02540"
     CDS             complement(2029..3567)
                     /locus_tag="VM841_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89093.1"
                     /translation="MRIRFRAVGAALALGAVAISVLGVAAPARASAAMQFNNLSSLGT
                     QGEIRARSVFQDGTGATVLQAGVPTDPKTNPNRTIGDAVAAVATPALNTPGPRLLGNN
                     PFAGDGHTHYHAGDTRLLINDVTGFALGDTLLVGSPYCNFAFRSLNCKSSVPAGNEQP
                     YGQVVHVVGGDINQTGPGILEIFPGLTGAPQCTIDYGFGPCAMLEGVQVTKTPTVYQG
                     QQLRIDIPHASLLNGGANPVAVTADFVPPQGTNDPSFPRRPLTLSRDTGATSAIVSNT
                     CPCGGGFFSFYVPKEIFVTQTIGNTFNVDLGHTWYQIIVTARDTVTGAIRSDGVLRLK
                     PNVVAITEFAPDRNPIEVTSAVNLRGRLRDNSSAAALTRRPVGDVIVDVVLTKPSGVR
                     SVYQTTSCVDTDPGPADRCGSDPLGTQPHGHGAFQIRVGGLQGLFVGIPGVGQSTLNC
                     SGQVILSQPNTYPNLLNVPGCVASPFLSAMDTLEPGTYDALATLRGFSPPVARATTFD
                     VVLV"
     gene            3709..4452
                     /locus_tag="VM841_02545"
     CDS             3709..4452
                     /locus_tag="VM841_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89094.1"
                     /translation="MRTMISAYRRAEPRWIDGAVSEESDGLYLQALGLPAGWANSAVP
                     MRHLPDAEARLREARQWFADRGLTMSVNVEDGYPEVEAAARTLGLERGPPRRGMVREP
                     LPVPSVPSPGIPLRRAVAEDREAVATAQAAGFGMTIEFARLLQPESKFDSVTILLAVD
                     GDAPAGTAEIFIHDGIAGIGGVCTIPSHRGLGLGRELMVESIRVAADAGCEKVCLFAA
                     EGTDGFYRALGFRPAYSERTWSPPGTAPE"
     gene            4552..6156
                     /locus_tag="VM841_02550"
     CDS             4552..6156
                     /locus_tag="VM841_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL89095.1"
                     /translation="MMEQPLTLRLVLDRARTLFPDRGATTKTPRGLVRTTYREIGERC
                     ERLANALTALGVGAGDRVGSFAWNSTRHLELYLGVPCMGAVLHTLNIRLHPEQVAWIA
                     NHAEDTVIFVDESLLPVFEQIAPHLTTVKHIVIMADGDLPPTAIDGALSYEDLLANAD
                     PTFAWPDLDESSACAMAYTSGTTGNPKGVVYSHRSMTLHAFMSCMVDTNGIGEADVCL
                     PIVPMFHANAWGIPWASALSGANIVFPAQYMTPVDILELIQSERVTFTAGVPTIWLGA
                     LPEIQKGTYDLSSIDRLVIGGAAVPLSLIKAYDELGIRVLQGWGMTETGPLASLSRPL
                     STMDAAMSLEELQKVRAKQGLPVAGVELRIMGDDGVAPWDGSTFGEVQVRGNWVARTY
                     YNDERAPESWTQDGWLRTGDVAVVDPTGFVQLVDRTKDLVKSGGEWISSVDLENAIMG
                     HPKVAEAAVIAIPDDKWSERPMACVVVKPGEDLTAGELMQHLSDTVPKWWLPEKVAFI
                     DEVPKTSVGKFDKKVLRARYAEGTLS"
     gene            6191..7507
                     /locus_tag="VM841_02555"
     CDS             6191..7507
                     /locus_tag="VM841_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89096.1"
                     /translation="MTRAHGSFRFRLSAVLAAGAFVIAGAGPAMAEDPVPPDGGGSSG
                     SSGAAPAQPVFQEGARILTDGQEARVLLPGKRSTLELTFHNQGSSDVRGAVAVAEHSS
                     GATVAASRSEVGTIPAGGTAVARFDVTVDSETCTEFYGIGGQLTSDDLPEPAYFKAGF
                     PVACPGARLSLGEVTIGGGDGDGIPEPGETVTLSVMLYNAGADPAKGVTARLRIQSKG
                     VEVLTDTASWPDIASGETAWSLTPFVLRFAADAPRQEACSFDGREPGVVEPVPAPAED
                     KPGSSEPGAAGGGNPETPVSSDGNTGSGTASDTPLIEPAPPVGAEPAPMPMPAEPPRE
                     PEQPGEPGVAFEAMFQIETANDERFTLEFSNGMYCAYADGGDQKVDMGAGGTDAARLE
                     ASAPTGARSQGGPAVPAAAALLSVVAAMLVRQALTRGIRIPRPTRP"
     gene            complement(7479..7850)
                     /locus_tag="VM841_02560"
     CDS             complement(7479..7850)
                     /locus_tag="VM841_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89097.1"
                     /translation="MTIVTFAGDRIGDLRDELAGDVRAVDFQQMRLNALAVGNLFGSN
                     VLNMAILLFVDAAHTKGPVLTLLGQPEIVAGVGAILLMAIALAVIVHGAETRIARMEP
                     DALVLLLAYAGCVTAVWAAGS"
     gene            8091..8267
                     /locus_tag="VM841_02565"
     CDS             8091..8267
                     /locus_tag="VM841_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89098.1"
                     /translation="MVDALDDLTLEELRELARIFKMNFRDETRLTKQDIILVLDEVNP
                     GPLKDAVAAVRRRR"
     gene            8336..8560
                     /locus_tag="VM841_02570"
     CDS             8336..8560
                     /locus_tag="VM841_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HVL89099.1"
                     /translation="MPQRVYNIILIPRPEGGFTATVPALPDVVTEAETRPMALAMARD
                     AIEGYRETIRAKGWKLPRTEHHTIRIEVKE"
     gene            8557..8922
                     /locus_tag="VM841_02575"
     CDS             8557..8922
                     /locus_tag="VM841_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HVL89100.1"
                     /translation="MSEIRLVTITDTITLTELAEIAEGSFGDLVKAVVDIDQRRMAIG
                     GELHADEEHILIEGGSKQPNLWGINLYPAEHGTENWLEYDSMINLRPSQKNRSRSVED
                     PKIREIIAEIVEQLVATND"
     gene            8915..9253
                     /locus_tag="VM841_02580"
     CDS             8915..9253
                     /locus_tag="VM841_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89101.1"
                     /translation="MTETTLHSNLAAGRWQTLSLVEQLGNIGSEVGRAIRAKARGNSE
                     RLQAALDRALELFDLTVNDERWAHRLKEVLRAREVVCDFLAGDNIYGATGESLDAYFL
                     PFAMATRRTT"
     gene            complement(9502..9897)
                     /locus_tag="VM841_02585"
     CDS             complement(9502..9897)
                     /locus_tag="VM841_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HVL89102.1"
                     /translation="MRVLWALTGRAPRLERVAAEELMCDATVVPLVFDGGRVVEVGDE
                     TTRFSPKVRRAIIARDQGCRAPGCRMPAQWTDIHHIVPGVGSSADDGVLHCRADHRRA
                     HRQRWKITLHPDGTVEYRIRGRTYLSRPP"
     gene            complement(9932..10642)
                     /locus_tag="VM841_02590"
     CDS             complement(9932..10642)
                     /locus_tag="VM841_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89103.1"
                     /translation="MLDVSISASARDRATLVADALHRADAAMAEAIRLLGSGNGIAAA
                     TGLPAERILQLDGRLTGADARMLSAASDVLRFMPSLTAAFDCGVVSWSQVRSIVCAVR
                     NVPSAVRDQIDTLIAEHADTLSPPSRTGSSKWWTMPCCGYVPAGSRRGRTGRSPQSPS
                     PSKAASTAGPASTASSVPSRQRRSWKLWMRMRTRLVIPTPRMRQAARANARSRSYASA
                     NPAYPVPGPVPAPRTGNG"
     gene            complement(10685..>11047)
                     /locus_tag="VM841_02595"
     CDS             complement(10685..>11047)
                     /locus_tag="VM841_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVL89104.1"
                     /translation="DSSCAAAHEYARRWDAAFQSVDVIALPATPVGAPLHFATTARIG
                     GDEVPVHGGLLSSKTRQINLAGVPAISIPCGFDENLMPFGLQLAAPRGHEGRLLAVAR
                     VYQSRTDWHRRRPAQAWV"
BASE COUNT         1832 a         3669 c         3794 g         1752 t
ORIGIN      
        1 gggaacggcc gcggcggctt cgacgaactg ctccgtggcg cgccatcgtc cttccaagaa
       61 gtacgcggcc gttcccgctc cgccggcggg cgggtcgagc ggcacgagat agtcgtgcgg
      121 ccggtcgcgc acgaagcctg actcgttgcc gaggaacttc tgctgttcgc cgcgcagcca
      181 tcccaggtag atctcgggcg tgatcccgcc gctggggccc ttcacggtca cgttcgtggg
      241 cgggggaagc gccacgcccg cctcgtcgag cagcgcgcgg atcgcccgct cgatctctgc
      301 ggcgccgcct tcgccgaccc ggtcgtagcg gatgcggccc tcggcgtcga tcaggtacac
      361 gtgcggccag aagcgattgc gaaacgcctc ccaggtcttg tgatcgttgt ccatcgcgac
      421 cggccacacg acgctgtgcg ctcgcatcgc cttgcgcacg ttgtcggggc tcttttcgaa
      481 gtcgaactcg ggcgagtgga tgccgatcac ctcgaggccc gcgttcttgt aggtgtcgta
      541 gaaagcgcgg atctcgggaa aggtgcgcac gcagttcacg cacgagaagg tccagaagtc
      601 gatcagcacg accttgccgc gcagcgcctt cagcgtcatg ggctccgcgt tgaaccactg
      661 cgacagaccc acgagctcgg gtgcgggatc gtttcggaac agcagcgtgt ctcccgcgcg
      721 gcccggccgg ttcgatccgc tggccgtgat ccggaacgcc gcaacgccga ccgcgacgag
      781 caaggcgacg acggcggcga cgatgcgagg gtcccggcgc atgatcccaa agcctacgcg
      841 gccggcggct gcgcggcggg gcggacgccg gccggcgcac cgaccctgcg gccgggcccg
      901 tgcagccggg cggagattaa atgaggccgg ccccttgcgg gaaccggcct catgcatcgg
      961 ctcgtgcctt ggtgtgggga cgggcgcggt cgcccgtccc cacgcggatc gcgccctatg
     1021 ccacctccgc ctcggcgttc gcaccggcgg cgacctcgac gggtcgcttc ggcggacggc
     1081 ctcggcgcgg cttgcgcgcg atcggagctc cgcggtcgaa cagctcgccg ccccagactc
     1141 cccagggctc gcgacgccgg acggcgccct cgtagcaggg aaggcgcagc gggcagtcgc
     1201 cgcagacttg ctttgccagc gagatctccg tctcgtcctc gctgaagaaa aggtgaagaa
     1261 ggctggcgtc tccggtggca catcgtgcct cggtccaact gacggtcgtg tccactgtcc
     1321 ctcccttcac gtgggtctcg gcctgccggg aagctcctgc tcagcttccg ggccttccga
     1381 gtcccgggtg ttgccctgtg cggggccccg gggcgtttcc ccgggtccga cccctgaaat
     1441 gcgatcctga aatgcagccg ggccgccctc gtgggcggcc cgggtgtctc ggtgtgagag
     1501 attctcctgt taggagtctc ccctttccga tcgcccgggc ttcgccggct tccacgagtt
     1561 gatcccgacc acggcaagca cggcagcagc ctggcagcgc gtgtgcgcgt ccatcgccgt
     1621 ggtcccaccg aagaagcgaa tcatcgtgat agccagtcct ttctctgccc cgaacccaat
     1681 gttcgcggcc tccccgggcc tttggcccga agcgccgaca gtctgccagg tccgcttgtt
     1741 ccacgcaacg gaattaaacg cccgaggtgt gacattttcc gtccgccctg ttcagcgacc
     1801 ctgttctgta tttccgacgc cggcgggccc gcagatgttc ccggcccgag ccaaaatctc
     1861 gaaacctgca ggctcgctcc cgagcgacga ccggccccgt gacaccttga cccgaaactc
     1921 ccggaagggg gcgaaccggg ggggccggcc cgcgtcgcct gaaagaacac gggaagacga
     1981 tcgcggcccg ccgcatcgga tgccggcggg ccgcgatcgt cttcggagct agaccaggac
     2041 cacgtcgaag gtggtggccc tggcgacggg cgggctgaag ccgcgcaggg tcgcgagcgc
     2101 gtcgtaggtt cccggctcca gcgtgtccat ggccgaaagg aacgggctcg ccacgcagcc
     2161 cggaacgttg agcaggttcg gataggtgtt cggctgcgac aggatcacct gacccgagca
     2221 gttgagcgtg gactggccca cgccggggat cccgacgaac aggccttgca gcccgccgac
     2281 ccggatctgg aaggctccgt ggccgtgcgg ctgggtcccc agcgggtcgg agccgcaccg
     2341 gtcggccggg ccgggatcgg tgtccacgca cgaggtggtc tggtagacgc tgcggacccc
     2401 cgacggcttg gtcaggacca cgtccacgat cacgtccccg accggccgcc gcgtcagcgc
     2461 cgccgcgctc gagttgtccc gcagccgccc gcgcaggttg accgcggacg tgacctcgat
     2521 cgggttgcgg tcgggcgcga actcggtgat ggcgacgacg ttcggcttca ggcgaagcac
     2581 gccgtccgag cggatcgcgc cggtcaccgt gtcacgggcg gtcacgatga tctggtacca
     2641 cgtgtgaccg agatcgacgt tgaacgtgtt gccgatggtc tgtgtgacga agatctcctt
     2701 cggcacgtag aaggaaaaga aaccccctcc gcacggacag gtgttgctga cgatcgccga
     2761 cgtcgcgccg gtgtcgcggc tcagggtcag cggccgccgc ggaaacgagg gatcgttcgt
     2821 cccttgcggc ggcacgaagt cggccgtgac cgccaccggg ttcgcaccgc cgttgagcag
     2881 tgacgcatgc ggaatgtcga ttcgcagctg ctggccctga tacacggtcg gcgtcttggt
     2941 gacctgcacg ccctcgagca tggcgcacgg accgaacccg tagtcgatcg tgcattgcgg
     3001 cgccccggtg agccccggga agatctcgag aattcctggg cccgtctggt tgatgtctcc
     3061 gccgacgaca tgcacgacct ggccgtaggg ctgctcgttg ccggccggca cggacgactt
     3121 gcagttgagc gagcggaacg cgaagttgca gtaaggcgat ccgacgagca gcgtgtcgcc
     3181 gagcgcgaac ccggtcacgt cgttgatcag cagcctggtg tcccccgcgt gatagtgggt
     3241 gtgcccgtcg ccggcgaacg ggttgttgcc gagcaagcgg ggtccgggcg tgttcagcgc
     3301 cggagtcgcg actgccgcga ccgcatcgcc gatggtgcgg ttcggattcg ttttggggtc
     3361 ggtcggcacg ccggcctgca gcacggtcgc gcccgtgccg tcctggaaga cgctgcgcgc
     3421 gcggatctcg ccctgagtgc cgagcgagga caggttgttg aactgcatcg cggcgctcgc
     3481 ccgggccggg gcggccacgc cgaggacgga gatggctacg gcgccgagcg caagcgcggc
     3541 cccaacggct cggaatcgga ttctcatgtc ggacgctccc cctggatcgg cttcgagacg
     3601 gtaggtttga ttggttgttc tcaccctgga gctttctatg tacttttccg ctttcgcggg
     3661 gtgaatcctt cttttcggga gaaacggaac ttggacgatt cggaagcgat gcggacgatg
     3721 atctcggcgt accgccgggc cgagccccgc tggatcgacg gcgccgtcag cgaagagagc
     3781 gacgggctgt atctgcaggc cctgggcctg cccgcgggct gggcgaactc cgcggtgccc
     3841 atgaggcatc tgccggacgc cgaagcccgg ctccgggagg cccggcagtg gttcgccgat
     3901 cgaggcctga cgatgtcggt caacgtcgaa gatggatatc ccgaggtcga ggccgccgcc
     3961 cgaacgctgg ggctcgagcg ggggccgccc cgtcgaggca tggtgcgcga gccgctgccc
     4021 gtgccgtccg tgccgtcgcc gggcatccca ctccgccggg ccgtggccga ggatcgcgaa
     4081 gccgtcgcaa cggcgcaggc cgccgggttc ggcatgacga tcgagttcgc gcggctgctg
     4141 cagcccgaaa gcaagttcga ctccgtcacg atcctgctgg ccgtcgacgg ggatgctccc
     4201 gccggaaccg cggagatctt catccacgac ggcatcgccg gcatcggcgg cgtgtgcacg
     4261 atcccgagcc atcgcggttt gggcctcgga cgcgagctga tggtcgagtc catccgcgtc
     4321 gccgcggacg caggctgcga aaaggtgtgt ctattcgcgg ccgaaggcac ggacggcttc
     4381 tatcgcgcgc tcggcttccg gcccgcctat agcgagcgca cctggtctcc gccgggaacc
     4441 gctcccgagt agcgcagcga ccgtcgtgct cttgctccgg agcggcccat cggaaacgat
     4501 gggctcgata ccgacgcagg ggggacgagg aagatgagct ttcccggact gatgatggag
     4561 cagccgctga cgctgcggct ggtcttggac cgcgcccgca ccttgtttcc cgatcggggc
     4621 gccacgacca agacgccgcg cgggctggtg cggaccacct atcgcgagat cggcgagcgc
     4681 tgcgagcggc tggcaaacgc gctgacggcg ctgggcgtcg gcgcgggcga ccgggtcgga
     4741 tcgttcgcgt ggaactcaac ccggcatctg gagctgtatc tgggggtgcc gtgcatgggc
     4801 gcggtgctgc atacgctcaa catccggctg catcccgagc aggtcgcctg gatcgccaac
     4861 cacgccgagg acacggtgat cttcgtcgat gagtcgctgc tgccggtctt cgagcagatc
     4921 gcgccgcacc tgaccacggt caagcacatc gtgatcatgg ccgacggcga cctgcccccg
     4981 accgcgatcg acggagcgtt gtcgtacgag gatctgctcg cgaacgcgga tccgacgttc
     5041 gcctggccgg atctggacga gtcgtcggcg tgcgcgatgg cgtacacgtc gggcacgacc
     5101 ggcaacccga aaggcgtcgt ctactcgcac cggtcgatga cactccacgc gttcatgtcg
     5161 tgcatggtgg acaccaacgg catcggcgaa gccgacgtgt gcctgccgat cgttccgatg
     5221 ttccacgcca acgcgtgggg catcccgtgg gcgtcggcgc tgtcgggcgc caacatcgtg
     5281 ttccccgcgc agtacatgac gccggtcgac attttggagc tgatccagtc cgagcgcgtc
     5341 acgttcacgg cgggtgtgcc cacgatctgg ctgggggcgc tgcccgagat ccagaagggc
     5401 acctacgacc tgtcctcgat cgaccggctg gtgatcggcg gcgcggcggt gccgctgtcg
     5461 ctgatcaagg cctacgacga gctgggcatc cgcgtgctgc aaggctgggg catgaccgag
     5521 acgggcccgc tcgcgtcgct gtcgcgtccg ctgtcgacga tggacgcggc catgtcgctc
     5581 gaggaattgc agaaagtgcg cgcgaagcag ggtctgccgg tcgcgggcgt cgagcttcgc
     5641 atcatgggcg atgacggcgt tgcgccgtgg gacggcagca cgttcggcga ggtgcaggtg
     5701 cgcggcaact gggtggcgcg cacctactac aacgacgagc gcgcgcccga gtcctggacc
     5761 caggacggct ggctgcgcac cggcgacgtg gcggtggtcg atccgaccgg cttcgtgcag
     5821 ctggtggacc gcaccaagga tctggtcaag tccggcggcg agtggatcag ctcggtggat
     5881 ctggagaacg cgatcatggg ccacccgaag gtcgccgaag ccgccgtcat cgcgatcccc
     5941 gacgacaaat ggtcggagcg gccgatggcg tgcgtggtgg tcaagcccgg cgaagacctg
     6001 accgccggcg agctgatgca gcacctgtcg gacacggtcc cgaagtggtg gctgcccgaa
     6061 aaggtcgcct tcatcgacga ggtgcccaag accagcgtcg gcaagttcga caaaaaggtc
     6121 cttcgggccc gctacgccga gggcacgctg tcgtagggaa cctttcggcc cggccgcgcg
     6181 tcgaacgatc atgacgcgtg cacatggatc cttccggttt cgcctgtcgg ccgtcctggc
     6241 cgcgggcgca ttcgtgatcg caggggccgg cccggccatg gccgaggatc cggtcccgcc
     6301 ggacggcggc ggatcatcgg gcagcagcgg cgccgctccg gctcagccgg tctttcagga
     6361 aggcgctcgc atcctgaccg acgggcagga ggcccgcgtc ctgctgccgg gcaagcgttc
     6421 gacgctcgag ctgaccttcc acaaccaggg ttcgagcgac gtgcgcggcg cggttgcggt
     6481 cgcggagcac tcgtccggcg cgacggtggc cgcgtcccgg tcggaggtcg gcaccatccc
     6541 tgccggcggc accgcggtgg cccgcttcga cgtgaccgtc gattcggaaa cgtgcaccga
     6601 gttctacggc atcggcgggc agctgacgtc cgacgatctg cccgagccgg cgtacttcaa
     6661 ggcaggcttc cccgtcgcgt gccccggcgc gcgactgtca ttgggtgagg tcaccatcgg
     6721 cggcggcgac ggcgacggca tccccgagcc gggcgagacc gtaacgctga gcgtcatgct
     6781 ctacaacgcg ggcgccgacc cggcgaaagg cgtcaccgcg cgacttcgga ttcaaagcaa
     6841 aggcgtcgag gtcctgaccg acaccgcatc ctggcccgac atcgcgtccg gcgagaccgc
     6901 atggtcgctc actccgttcg tgcttcgctt cgccgccgac gcgccccgtc aggaagcctg
     6961 cagcttcgac ggccgcgaac cgggcgtcgt cgaacccgtc cccgcccctg ccgaagacaa
     7021 gcccggctcg agcgagcccg gcgcggccgg gggcggcaat cccgagaccc cggtgtcctc
     7081 ggacggcaac accggcagcg gaacggcgag cgacacgccg ctgatcgagc cggccccgcc
     7141 ggtcggcgcc gaacccgcgc cgatgccgat gccggccgag ccgccgcgcg aacctgagca
     7201 acccggtgag ccgggcgtcg cgttcgaagc gatgttccag atcgaaacgg ccaacgacga
     7261 acgcttcacg ctcgagttct cgaacggcat gtattgcgcg tacgcggacg gcggcgacca
     7321 gaaggtggac atgggggccg gcggcacgga cgcggcacgg ctggaagcat cggctccgac
     7381 cggcgcacga tcgcagggcg gcccggccgt ccccgcggcg gcggcgctgc tatcggtcgt
     7441 ggcggcgatg ctggtgcggc aagcgctgac gcgcgggatc aggatcccgc ggcccacacg
     7501 gccgtgacgc agccggcgta ggcgagcagc aacaccaacg catcgggttc catgcgcgcg
     7561 atgcgcgtct cggcgccgtg cacgatgacc gcaagcgcga tcgccatcag cagaatcgcg
     7621 cccacgccgg cgacgatctc gggctggccc agcaacgtca gcaccggacc tttggtgtgg
     7681 gcggcgtcca cgaacagcag gatcgccatg ttgagcacat tgctgccgaa caggttgccg
     7741 acggccagcg cattcaggcg catctgctgg aaatctacgg cgcggacgtc tcccgcgagc
     7801 tcgtctcgaa ggtcaccgat gcggtcgccg gcgaaggtga cgatcgtcat atcgacacct
     7861 accggagcgt ctcggatagc ccgaacaccg agaagatcga ccacacatcg acgagtgact
     7921 cgccgggcta ccacaacacg ccggtaaaca ttccggttag cgttgaaaag cagtaacatc
     7981 aggctcagaa tgaagagggg gagatctcgc cctcttcatt cgtttattgg cggagtatct
     8041 ccatggggca aacaacattc gaaaactggg acgtaaacga gtggcgcgcg atggtcgacg
     8101 ccctggacga cctgaccttg gaagagctga gagaactggc ccgcatattc aaaatgaact
     8161 tccgtgacga aactcggctc accaaacaag acatcatcct ggtcctcgat gaagtcaacc
     8221 ctggccctct aaaggacgct gtcgcggccg ttcgccggcg ccgatagtca ccaaacccct
     8281 ataaatccag ggttttccac ggtataatgg tggcaccgcc aagaaggaga atccagtgcc
     8341 ccaacgcgtc tacaacatca tcttgatccc ccggcccgaa ggtggcttta ctgccaccgt
     8401 gcccgcgctt ccagacgtag tgaccgaagc tgagactcgt ccgatggctc tagcgatggc
     8461 gcgcgatgct atcgaaggct accgggaaac catccgcgcc aagggatgga aacttccgcg
     8521 gacggagcac cacacgattc gaatcgaggt gaaggagtga gcgagattcg cttggtgacg
     8581 atcactgaca cgatcaccct gacggaactg gcggagatcg ccgaaggctc gttcggtgat
     8641 ctggtcaaag ccgtggtcga catcgatcag cgccggatgg ccatcggcgg cgagctgcat
     8701 gccgacgagg agcacatcct gatcgagggc ggttccaagc agccgaacct atggggtatc
     8761 aacctctacc cggccgagca tgggaccgag aactggctcg aatatgactc gatgatcaac
     8821 cttcggccga gccagaagaa ccgttcccgc tcggtcgaag acccgaagat ccgcgagatc
     8881 atcgctgaga tcgttgagca gctggtggcg accaatgact gagacgaccc ttcactcgaa
     8941 tctagccgcc ggtcggtggc agacgctgtc gctcgtcgag cagcttggca acatcggtag
     9001 cgaggtcggc cgcgccatcc gcgccaaagc ccgcggcaac agcgagcggc tgcaagcagc
     9061 gctcgaccgc gccctagaac tcttcgacct gacggttaac gatgagcgct gggcacaccg
     9121 gctcaaagag gtcctgcgcg cccgtgaggt cgtctgcgat ttcctggccg gcgacaacat
     9181 ctacggagcc actggcgaat cactcgacgc ctacttcctt cccttcgcca tggccactcg
     9241 ccgtactacg taggagtttc ctcaaccgaa aggccgcagc atcagctgcg gccttttctc
     9301 gtccaaactc tcgatgtggt ttgagtatcg ggaatttctt cgttgtgttt cccctgatgg
     9361 ggctgggcca gttcttctgt cggcggaggt gaacggattt cgaaccggcg acgggcatgg
     9421 caatgtggcg gcgccggtag gcggagcgcc gtggttggtg gccagggtac gttcgtggtg
     9481 cggggagaaa cttcgggccg gctatggggg gcggctgagg taggtgcggc cgcggattct
     9541 gtattcgacg gttccgtcgg ggtgcagggt gatcttccag cgttgccggt gggctcggcg
     9601 gtggtcggcg cggcagtgga gcaccccgtc gtcggcgctg cttccgaccc ctggcacgat
     9661 gtgatggatg tcggtccact gcgcgggcat gcggcagccg ggcgcgcggc atccttgatc
     9721 gcgggcgatg atcgcgcgac gcaccttcgg cgagaacctc gtcgtctcgt ccccgacttc
     9781 gacgacacgg ccaccgtcga agacgagggg aacgacggtc gcgtcgcaca taagctcttc
     9841 ggcggcgacc cgttccaagc gcggcgcgcg gccggtgagg gcccacagca cgcgcatcgc
     9901 tccatcgcgt ccgtccaaga acgatgcggc gtcaaccgtt gccagtacgc ggggccggga
     9961 cgggtcccgg taccggatag gccggattcg cagatgcgta cgaacgactc cgcgcgttgg
    10021 cgcgcgcggc ttggcgcatc ctcggcgtcg ggatgacgag gcgcgtccgc atgcgcatcc
    10081 aaagcttcca ggaccgtcgc tgtcgagacg ggaccgaact cgccgtagat gctggacccg
    10141 ccgtcgaagc ggccttggac ggcgagggac tctgcggcga cctgccggtt ctgccgcgac
    10201 gcgagccggc cgggacatag ccgcagcacg gcatcgtcca ccacttcgat gagccggtac
    10261 ggctcggcgg tgacaaggtg tccgcatgct cggcgatgag cgtgtcgatc tgatcacgca
    10321 ccgctgacgg cacgtttcgc accgcgcaca cgatcgaacg cacctgcgac cacgacacca
    10381 cgccacaatc gaacgcggcc gtgagcgacg gcatgaaccg cagcacgtcc gacgcggcgg
    10441 acagcatccg cgcgtcggca cccgtcaaac ggccgtcgag ttggaggatc cgctcggccg
    10501 gcagacccgt cgcggccgcg atcccgttgc cgctccccaa cagacgaatc gcttcggcca
    10561 tcgcggcatc ggcgcgatgc agcgcgtcgg cgaccagcgt cgcgcggtca cgcgcagagg
    10621 cagaaatgga aacgtcgagc atgcttgcaa ctgtagagca tgccctacga cagaaacgtc
    10681 agggttaaac ccaggcttgg gcggggcggc ggcggtgcca gtcggtgcgg gattgatata
    10741 cgcgcgccac ggcgagcagc cggccttcgt ggccgcgagg agcggcaagt tgcaggccga
    10801 acggcatcag gttttcgtcg aagccgcacg ggatcgagat cgcgggcaca ccggccagat
    10861 tgatctggcg cgtcttcgac gacagcaggc cgccgtgcac cggcacctcg tctcccccga
    10921 tgcgcgcggt ggtcgcgaag tgcagcggcg caccgaccgg cgtggccggc aacgcgatga
    10981 cgtccacgct ctgaaaagcc gcatcccagc ggcgcgcata ctcgtgcgcc gcggcgcacg
    11041 agctgtc
//
LOCUS       DATLFO010000075         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_81025, whole genome shotgun sequence.
ACCESSION   DATLFO010000075 DATLFO010000000
VERSION     DATLFO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81025"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1319)
                     /locus_tag="VM841_02600"
     CDS             complement(162..1319)
                     /locus_tag="VM841_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89105.1"
                     /translation="MTDYDPRSETGIPTEPVGSLPRPSKLQAAYAEYDEGKISKEDLE
                     KLQDEAAKDSIERMEATGSPIISDGEQRWSSFATYPIADTLAGTGLAPNLGPGGQFFA
                     IFADGHGRQLPKLTGGPLKYNFYAADTLKKSLPYASKPMKQAVIAPSMLALLYPLKDP
                     VEGYSREEFEEDIVQECTTDIRKAFEAGAARVSIDFTEGRLATREDPRNPWTGAGLLP
                     HFIELNNRVLANFSDEERKKIGIHTCPGGDRDSVHSADVPYVNLLPEMFKINAGYFLI
                     QLASERDRDSVYQLIGEHSREDQMCYIGVTVTQSPRPESAQEICDAIVRAANFIPKER
                     LGTTDDCGFSPFSIDEKPNHGSPDYAREIAFQKIASRVEGTRRAAEKLGVG"
     gene            complement(1458..>2534)
                     /locus_tag="VM841_02605"
     CDS             complement(1458..>2534)
                     /locus_tag="VM841_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVL89106.1"
                     /translation="ALDDALADAGPNTGRIEAIFFANALSGLMTGQECVRGQVALRHS
                     RYGGIPTVNVENACASGSTALHLACMAIESGMYETVAVVGSEKMSHPDKRRSIQALAG
                     AADVDLLADAPEDHSVFMDSYAERARARIERNGWNPADFARVVVKNRGHAVHNPGAQF
                     RNTMTVEEVLSSRMITEPLTLPMCSPIGDGAGALIVTGTPNGAAAPIRIRASALSATG
                     GPAPGSVVQRASVTAREQSGLSAEDVDVIEVHDATASAELEAYEHLGLAAPGEGNRLI
                     VEGTTALGGALPVNTGGGLLSRGHPVGATGVLQVVELVAQLRGRAGERQVDLDPRLAL
                     AQNAGGFVEGDNAVAVITVLERER"
BASE COUNT          400 a          895 c          797 g          442 t
ORIGIN      
        1 gggcgggact gtaggagcac accggccgac ctgctcgccg ccgacgtcga ccaatcgaag
       61 gctcctggag agacggtaga tcctccgtca cgacgagatg caccagcggt ccggcccagc
      121 cggccgctga tacatccgcc gaggacgggt gatcgccggc ctcagccgac gccgagcttc
      181 tccgctgcgc ggcgggtgcc ctcgacgcgg cttgcgatct tctgaaaggc gatctcacgg
      241 gcgtagtccg gtgagccgtg gttgggcttc tcgtcgatcg agaacggcga gaagccgcag
      301 tcgtcggtcg tgcccagacg ctccttggga atgaagttcg cggcgcgcac gatcgcgtcg
      361 cagatctcct gtgcgctctc gggtcgcggc gactgcgtca ccgtgacgcc gatatagcac
      421 atctgatcct cgcggctgtg ctcgccgatc aactggtaca cgctgtctct gtcgcgttct
      481 gaggccagct ggatgaggaa gtagccggcg ttgatcttga acatctccgg caacaggttg
      541 acgtacggca cgtcagccga gtgaaccgag tcgcgatcgc cgcccgggca cgtatggata
      601 ccgatcttct tgcgctcctc gtcagagaag ttcgccagca cccgattgtt gagctcgatg
      661 aaatgcggca acaggcccgc accggtccac gggttgcgcg gatcctcacg cgtcgccaag
      721 cggccctcgg tgaaatcgat cgagacacgc gcagccccag cctcgaaggc cttgcgaatg
      781 tccgtcgtgc actcctgcac gatgtcctcc tcaaactcct cgcgcgagta accctccacc
      841 ggatccttca gcggatacag caacgccagc atcgacggcg cgatgacagc ctgcttcatc
      901 ggcttcgacg cgtaaggcag cgacttcttc agcgtgtccg ccgcgtagaa gttgtacttc
      961 agcgggccgc ccgtgagctt gggcagctga cgcccgtggc catcggcgaa gatcgcgaag
     1021 aactgaccgc ccggcccgag gttcggcgcc aaaccggtac cggcaagcgt gtcggcgatc
     1081 ggataggtcg caaacgacga ccaacgctgc tcgccatcgg aaatgatcgg cgaccccgtc
     1141 gcctccatcc gctcaatcga gtccttcgcc gcctcgtcct gcagcttctc cagatcctcc
     1201 ttggagatct tgccctcgtc gtactccgca taagcagcct gcaacttcga aggccgcgga
     1261 agcgaaccaa ccggctcagt cggaatgccc gtctcggatc tcgggtcgta atcggtcatg
     1321 cgctctcctc ctagtgggtc tctctcctgc taccgcccgc ctgctctgcc ctgcctggcc
     1381 cagtgcagca ttgcttccgt gacacagcct ctctcacccg accatgcgcc gcacctgtcg
     1441 tccccatggc cttcgtgcta tcgctcccgc tcgagcacgg tgatcacggc gactgcatta
     1501 tcgccctcga cgaagccgcc ggcgttctgg gcgagcgcca gccggggatc gagatcgacc
     1561 tggcgctcgc ccgcgcgccc acgcagctgc gcgaccagct cgacgacctg caggactcca
     1621 gtcgcgccga ctggatgacc gcgcgagagc aggccgccgc cggtgttgac cggcagcgcg
     1681 cctccgagcg cggtcgtgcc ctccacgatc aggcggttgc cctcgcccgg cgccgccagc
     1741 ccgagatgct cataggcctc gagctccgcg gaagcggtgg catcgtgtac ctcgatgacg
     1801 tcgacatcct cggcgctcag tccgctctgc tcgcgggcgg tcacgctcgc gcgctgcacg
     1861 accgatcctg gagccgggcc gccggtcgcg ctcagtgcgc ttgcgcggat gcggatcggc
     1921 gcagcggccc cattcggcgt gccggtcacg atcagcgcgc cggctccgtc gccgatcggc
     1981 gagcacatcg ggagcgtgag cggctcggtg atcatccggc tcgacagtac ttcctcgacg
     2041 gtcatcgtgt ttcgaaactg agcgccgggg ttgtgaaccg cgtggccgcg attcttgacg
     2101 acgacgcggg cgaagtccgc cgggttccag ccgttgcgct cgatccgcgc gcgagcacgt
     2161 tcggcgtagc tgtccatgaa cacgctgtga tcctccggag cgtcagcgag cagatcgacg
     2221 tcggctgcgc cggcgagcgc ctggatcgac cgccgcttgt ccgggtgcga catcttctcg
     2281 gatccgacga cggcgacggt ctcgtacatg cccgattcga tcgccatgca ggcgaggtgc
     2341 agcgccgtcg acccgctcgc acacgcgttc tccacgttca ctgtcgggat ccccccgtac
     2401 cgcgagtggc gcagggcgac ctgtccgcgc acgcactcct ggccggtcat caggcccgac
     2461 agcgcgttgg cgaagaagat cgcctcgatg cggccggtgt taggccccgc gtcggcgagc
     2521 gcgtcatcga gcgc
//
LOCUS       DATLFO010000076        10345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_51558, whole genome shotgun sequence.
ACCESSION   DATLFO010000076 DATLFO010000000
VERSION     DATLFO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51558"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1162)
                     /locus_tag="VM841_02610"
     CDS             complement(23..1162)
                     /locus_tag="VM841_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89107.1"
                     /translation="MNLTAMSLAILMAVAGSGGATRASTATASTTASTTASTAASTTA
                     IAAVAAEADWILTAIADDGAIALTPAQDVVVPYDANIAAWSLARAAAVTRDPRYAAAA
                     WRWLDWFAARQDPFTGIVTNHVRDAEGWRATGEVDATDSVAATHMIALGELVAATGDV
                     VRLQRALPAMRRSMNALAITHDRTDGLYYALPGYEWKYTMDQAEVAAGLRAAAWMAWL
                     GGRWGDAAEAAVAAAEAQAAVEQLWSGATGAYDWGKHRDGTRAKTAWWRWYPSATAQA
                     WTVLFGVARADRVGAMMSELERNHPNWDRPEAMTFNDTGGQPVGYWPLAGVALARAGS
                     ALRALEGAHRIRAAADAAGRVYPFTPAAAGGLILLFAEPSVLALS"
     gene            1242..1748
                     /locus_tag="VM841_02615"
     CDS             1242..1748
                     /locus_tag="VM841_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89108.1"
                     /translation="MSNDEPPLSPPPAEPSGPDLSRLRRFSVDAKPPKPERDVHIGRL
                     AAFVGAALFVIVVPWLVFTRVGDSGPARPRATVSPSVSASPAGSPSPTVNPEAGVYEL
                     VGEVECVRIRIDAGTDKEILNCIKPGVRLESDGEMREADNFVWLHVEDPFKGIDGWVA
                     TQYVKRVS"
     gene            1745..2836
                     /locus_tag="VM841_02620"
     CDS             1745..2836
                     /locus_tag="VM841_02620"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVL89109.1"
                     /translation="MTSRFEALVCGDCGRVHPARLDGVCECGGLLAARYDLAGSESMP
                     RGGAMWSWTSLLPPCEPLTLGEPPVRLIERGDVLYADDGALPTGTFKARGAAVGVAMA
                     RALGATGVVLPTAGNAGAAWAAYCAEAGLACAVFAASDAPAGAVDQAREYGAAVSVVD
                     GSIADAGARAAALAAERGWHLAATFREPWRTEGKKTALLEIADALGGRAPDAAVLPVG
                     GGVGVVAWHKALAEARALGWVSGEMRLYAVQAEGCAPIVRAFEAGADASEPWPDPVTS
                     AAGIRIPSPLASRPVLRALRDSGGGAVAVSEAEIAAAADAFAEPVALEAAAAWAGYER
                     LRERGAFAPGETVVVYGTGTRERAARAAG"
     gene            complement(2833..2988)
                     /locus_tag="VM841_02625"
     CDS             complement(2833..2988)
                     /locus_tag="VM841_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89110.1"
                     /translation="MGVPDCKRCGRGMQQPGRYGEWVCDCCDMSARCRHVSRWTRLMR
                     RFAGRRV"
     gene            3073..4755
                     /locus_tag="VM841_02630"
     CDS             3073..4755
                     /locus_tag="VM841_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL89111.1"
                     /translation="MIRALKLIVVLVVAVAGLAPVPGRAEPVKLADVRISMSDGVDLV
                     GDVRLPSAGGTYPVILVMSPYGREDQTSPGALTADYLSHGYAHVNVDIRGTGKSDGAL
                     CLFCDREQQDVYEVVEWIAVQPWSDGNVGMQGGSYLGISQLLGAAKQPPHLKAIVPDV
                     PYTDTYRDIVWHNGIWNQQFMTLWTGLQAGLGFQGAAESRRLPDRAFNALALEFKNAP
                     LDGPTYWERSVYTKFDRILVPTLLRSGWFDGFSRGTIRNFQGIAATHKRLIMGPSPHT
                     PAGPPFDPLSPYAPVPPSPGAGDPALAWFDRFLKGIDNGIEHEAPVLYYDLGAFEWRS
                     ATTWPPPSSSMRTLHLSGERSGSIASLNDGTLADEAPTGAATYPSSYRSDPTVGVTEV
                     NGKWGNVAAAPFVRHDQTADAMRQLTYTTPALTEPLRLAGPMELRFHGETDQSDTDWI
                     VKVADVAPDGSSLLLTSGYLRASHRAVNDRRSLPADPWIENTTKLTVWPNDPTLFRID
                     IWPVAAELRPGHRLRIMISSTDVPNHEPLPHVSRNTVHHSARYPSELLLTVA"
     regulatory      4808..4963
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4989..5480
                     /locus_tag="VM841_02635"
     CDS             4989..5480
                     /locus_tag="VM841_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89112.1"
                     /translation="MRDVFALSAPQPSTGWGFRFSKRIALAMLMVVALGGAVPAHAAN
                     VTVKVRIAAGGHAGAIKDCAAVSVPAGANGLTVLGAAVAQGCIKSFQTASGGAYVTCI
                     DPGQASLCEVAGGLVTFWAVYDNGRASTTGISGFAASQGRELAFAYTNFLTCFATYPS
                     CPL"
     gene            5483..6694
                     /locus_tag="VM841_02640"
     CDS             5483..6694
                     /locus_tag="VM841_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HVL89113.1"
                     /translation="MSRLLRASVAVAMVMIVAPLSLARAEPGPAAASAAAWVADRQAA
                     DGGFFGSAQPADATAETIASLAAAGTPAARSAIGRALGRIRADGPSRASRPAYAGRIV
                     MGLVAAGRDPRSFDGTNYVARITAGYSAASSTYETGVYANALAILGLVAAREPVPSGA
                     VTYMRANQCSDGGFAHDPGCVPRGETDSTSIVVCALAGMGVGRDDLIVSRAREWLRAV
                     RAPGGGWPHFPGGPVNANSTGLALSALACLGERDAASLRVLRSLQMPSGGIRYDGDDA
                     EPNSYATVQSIPGLAGRGYPVRPATAVAGTKESAVPSPPSPAASSRAPAPAGVAAPAA
                     SPAGSGSPSSRPAAAENGPRNAPAALPPSPRDDGRLPPAVLASGLAVAGVAAAFAGAR
                     VPAAVRRRRHR"
     gene            6691..7353
                     /locus_tag="VM841_02645"
     CDS             6691..7353
                     /locus_tag="VM841_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89114.1"
                     /translation="MRVVAAAIVLAVAAGGSTPATTPATGPASDPCEHRPVPVAAVQS
                     GERHAGLIVESPSGTRQFCIAFSAQEEAAGFSGVDLLQRAEHDLASVCRIGDDGCGDP
                     GDCFCQCHDTGAASCRFWGYYTLGEQGAWSFAQQSPSLRRIRDGDVDGWRFGAHAGGA
                     GEPPAVPEARCANVAVARTAGGPARRGGGVPLGVIAASVVALGFAVGIAYGVRRRPGG
                     ST"
     gene            7350..8480
                     /locus_tag="VM841_02650"
     CDS             7350..8480
                     /locus_tag="VM841_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HVL89115.1"
                     /translation="MTARLHPAAWMVWTASASAVILSLDNPVLIVLSLGALGFVATSL
                     APDRGPFGLMLKIGLIALAARTVLFALTGHPSGEILFTMPQVRVPALLGGFTLGGPVT
                     ASVVVHSAIEGARLAALLCCFGVFLSAVEATRVVRLMPRFLFEAGLIVAIAISFVPSL
                     ARTARDVRDARRIRGERVRGWRGTTLAIPILASTLERAVTVAESMEARGYGRVAGGDA
                     GREAFARLRTLAGVTLLTGGGLLAAVAGVRGGWMVFAAGGALAASGLRALSGLSARTR
                     YRRDRMRGADAAVTVAAVAVAVVSLGLRGAWSYAAYPSVTMPAVPVAGVLVVACLASP
                     VAIVAWHARSLRRASRDPAGAAAVGAAPVLPADPLPVAGGQS"
     gene            8477..10066
                     /locus_tag="VM841_02655"
     CDS             8477..10066
                     /locus_tag="VM841_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL89116.1"
                     /translation="MIRFEGVTFTYPRAASPALAGVDMTWGREELVLVAGVSGSGKST
                     LLRAANGLVPHFSGGTLDGRVTVAGLDTRYDKPRALSGAVGFVAQDPEGGSVASVVED
                     EIAFFLENLGLDPPLMRKRVEETLDALAIAHLRERALDSLSGGERQRVAIAAVLVAAP
                     SVCVLDEPTSMLDPQSAEEVLQSVLRLSHEMGLGIVLSEHRLERVLAFADRVCVVEEG
                     RVRIGPVRDMLADSPVAPPVVRLARALGWTPVPVSVREARARAASLDVHPGRAAAGRP
                     APGGERIAVKNLSVALGGREVVRDLDLSVREGETVAVVGRNGAGKTTVLRAILGLVRR
                     VRGAVLLDGAQRDSVEDAATVAAYVPQAASRTLTRARSIDEIERTLRRKGVRDPLAPV
                     RILDDWGLSAFAERDPRDLSAGEATRLALAAATAHDPGIVLLDEPTRGMDEPEKHRLA
                     ERLAAWRHEGRAVVMVTHDVELIAEAATRVVLLADGEAVVDGPARSVLGESVMFSSQM
                     NKVFEDRRIATVADAVAAVTP"
     gene            10063..>10345
                     /locus_tag="VM841_02660"
     CDS             10063..>10345
                     /locus_tag="VM841_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HVL89117.1"
                     /translation="MTTIAAPTGRPPRASRAATVASRALLIAANAAAVAALLAPMIAG
                     ADPAAGREAAHATDGPILLALAVPALLAVALGEASGKRLDAKGIALLGVL"
BASE COUNT         1245 a         3700 c         3836 g         1564 t
ORIGIN      
        1 ggtcggggag tgagccggtg ggttaggaga gcgcgagcac ggagggttcg gcgaagagca
       61 ggatcagacc gcccgcggcc gcgggggtga aggggtagac ccgtcccgcc gcgtcggcgg
      121 ccgcgcggat gcggtgcgct ccctcgagag cgcgcaaggc gctgccggcc cgggcgagcg
      181 cgacgcccgc gagcggccag tagccgaccg gctggccgcc cgtgtcgttg aaggtcatcg
      241 cctcgggccg gtcccagttg ggatggttgc gctccagctc gctcatcatc gcgccgacgc
      301 gatccgctcg cgcgacgccg aacagcaccg tccaggcttg cgcggtcgcc gagggatacc
      361 accgccacca cgcggtcttc gcccgagtgc cgtcgcggtg cttgccccag tcgtaggcgc
      421 cggtcgcgcc ggaccacagc tgctcgaccg ccgcttgcgc ttcggcggcg gcgacggccg
      481 cttcggccgc atccccccag cggccgccga gccacgccat ccatgcggcg gcgcgaagcc
      541 cggccgcgac ctcggcctga tccatcgtgt acttccactc gtatccgggc agcgcgtaat
      601 agaggccgtc ggtgcggtcg tgcgtgatcg cgagcgcgtt catggaccgg cgcatcgccg
      661 gcagggcgcg ttgcagccgc accacgtcgc cggtggcggc gacgagctcg ccgagcgcga
      721 tcatgtgcgt cgccgcgacg ctatcggtcg cgtcgacctc gccggtcgcg cgccagccct
      781 cggcgtcgcg cacgtggttg gtgacgatgc cggtgaacgg gtcctgccgc gcggcgaacc
      841 agtcgagcca gcgccacgcc gcggcggcgt atcgcgggtc gcgcgtaacc gccgccgccc
      901 gcgcgaggct ccatgccgcg atgttcgcgt cgtagggcac gacgacgtcc tgcgcggggg
      961 tcagcgcgat cgcgccgtca tcggcgatcg cggtcaggat ccagtccgcc tccgccgcga
     1021 ccgccgcgat cgccgtcgtg ctcgccgccg tgctcgccgt cgtgctcgcc gtcgtgctcg
     1081 ccgtcgcggt gctcgctcgg gtcgcgccgc cgctgccggc aacggccatc aggatcgcga
     1141 gcgacatcgc ggtcaggttc atccggttct ggttctccct gatatgccgc cttccctgct
     1201 gtgagcgaag cggcgcccca tgtggaacca tgatgcgaat cgtgagcaac gacgagccac
     1261 ctctttcccc gccgccggcc gagccttccg gtccggacct atcccggctg cgccggttct
     1321 cggtggacgc caagccgccc aagcccgagc gcgacgtgca catcggccgg ctcgccgcgt
     1381 tcgtcggggc cgcgctgttc gtgatcgtgg tgccgtggct cgtcttcacc cgcgtgggcg
     1441 actcgggccc ggcgcgtccg cgcgcgacgg tctcgccgtc ggtgtcggcg tctccggccg
     1501 gctcgccgtc gcccaccgtc aatccggagg ccggcgtcta cgagctcgtc ggtgaggtcg
     1561 aatgcgtgcg catccggatc gatgccggga ccgacaagga gatcctgaac tgcatcaagc
     1621 ccggcgtgcg cctcgagagc gacggagaga tgcgggaagc cgacaacttc gtgtggctgc
     1681 acgtcgagga cccgttcaaa gggatcgacg gctgggtcgc cacgcaatac gtgaagcgcg
     1741 tgtcgtgacg tcgcgattcg aggccctcgt gtgcggcgac tgcggccgcg tccatccggc
     1801 aaggctggac ggggtgtgcg agtgcggcgg cctgctggcc gcgcgctacg accttgccgg
     1861 atccgagtcc atgccgcgcg gcggcgcgat gtggtcgtgg acgtcgctgc tgccgccgtg
     1921 cgagccactc acgctcggcg agccgccggt gcgtctgatc gagcgcggcg atgtgctcta
     1981 tgccgatgac ggcgcgttgc cgaccggaac gttcaaggca cgcggcgcgg ccgtgggcgt
     2041 cgcgatggcg cgcgcgctcg gcgcgaccgg cgtggtgctg ccgacggcgg gcaacgccgg
     2101 cgccgcctgg gccgcgtact gcgccgaggc cggtctcgcc tgcgccgtct tcgccgcgtc
     2161 cgatgcgccg gcgggcgcgg tcgatcaggc gcgcgaatac ggcgcggcgg tctcggtcgt
     2221 ggacggctcc atcgccgacg cgggtgcgcg ggcagcagcg ctcgccgcgg agcgcggctg
     2281 gcatctggcc gcgacgttcc gcgagccctg gcgtaccgag ggaaagaaga ccgcgctgct
     2341 cgagatcgcg gacgcgctcg gcggacgcgc gccggacgcg gcggtgctgc cggtaggcgg
     2401 cggcgtcgga gtggtcgcct ggcacaaggc gctcgccgag gcgcgcgcgc tcggatgggt
     2461 gagcggcgag atgaggctgt atgcggtcca ggcggaaggc tgcgcgccga tcgtacgcgc
     2521 cttcgaggcc ggtgcggacg catccgagcc gtggccggat ccggtcacga gcgcggccgg
     2581 catccggatc ccgtcgccgc tcgcgtcgcg gccggtgctg cgcgcgctgc gcgacagcgg
     2641 cgggggagcg gtcgcggtgt ccgaggccga gatcgccgcc gccgcggatg cgttcgccga
     2701 gccggtcgcg ctcgaggcgg ccgcggcatg ggcgggctac gagcggctgc gtgagcgcgg
     2761 tgcgttcgcc ccgggcgaga ccgtcgtggt ttacggaacc ggcacgcgag aacgcgcggc
     2821 gcgcgccgcc ggctagaccc ggcggccggc gaagcgccgc atcagccgcg tccaccgcga
     2881 cacgtgccgg cagcgcgcgc tcatgtcgca gcagtcgcag acccactcgc cgtaccggcc
     2941 gggctgctgc atgccgcggc cgcatcgttt gcagtcgggg acgcccatct cctcatccta
     3001 atgaagcctt gacagggccg cttccgtgac ggaaggtggc gatacgtcca ttttgtcctt
     3061 tggggggccg ccatgatccg ggcactgaag ctcatcgtcg tcctcgtcgt tgccgtcgcg
     3121 ggtcttgctc ccgttcccgg gcgcgcggag cccgtcaagc tggccgacgt tcgcatttcg
     3181 atgtcggacg gtgtcgatct ggtcggcgac gtccggctgc cctccgcggg cggtacgtat
     3241 ccggtcatct tggtgatgag cccgtacgga cgcgaggatc agacctcgcc cggcgcgctc
     3301 accgccgact atctctcgca cggctacgcg cacgtcaacg tggacatccg cggcaccggg
     3361 aaatccgacg gcgcgctgtg cctgttctgc gatcgcgagc agcaggacgt ctacgaggtc
     3421 gtcgagtgga tcgccgtgca gccctggagc gacggcaacg tcggcatgca aggcgggtcc
     3481 tacctcggaa tctcgcagct gctcggagcg gcgaagcagc cgccgcacct gaaggccatc
     3541 gtgcccgacg ttccctacac cgacacctac cgagacatcg tgtggcacaa cgggatctgg
     3601 aaccagcagt tcatgacgct gtggaccggc ctgcaggccg gcctcggctt tcaaggagcc
     3661 gccgagagcc gccggcttcc cgatcgcgcc ttcaacgcgc tggcgctcga gttcaagaac
     3721 gcgccgctgg acgggccgac ctattgggag cgcagcgtct acaccaagtt cgaccgcatc
     3781 ctggtgccga cgctgcttcg ctccggctgg ttcgacggat tctcgcgcgg caccatccgc
     3841 aactttcaag gaatcgccgc cacgcacaaa cgtttgatca tggggccgag cccgcacacg
     3901 ccggccggtc cgcccttcga tccgctcagc ccgtatgcgc ccgtgccgcc ctcgcccggg
     3961 gccggcgacc ccgcgctcgc atggttcgac cggttcctca aaggcatcga caacggcatc
     4021 gagcacgagg cgccggtcct gtactacgac ctcggcgcgt tcgagtggcg cagcgcgacg
     4081 acgtggccgc cgccgagctc gtcgatgcgc acgcttcact tgtcgggtga gcggtccggc
     4141 tcgatcgcat cgctcaacga cggaacgctc gccgacgaag cgccgacggg cgccgcgacg
     4201 tatccatcgt cgtatcggtc ggatccgacc gtgggtgtta ccgaggtgaa cggaaagtgg
     4261 ggcaacgtcg ccgccgcgcc gttcgtgcgg cacgatcaaa ccgccgacgc gatgcgtcag
     4321 ctgacctata cgacgcccgc attgaccgag ccgcttcgcc tcgccggtcc gatggagctt
     4381 cgcttccacg gcgaaaccga tcagtccgac accgactgga tcgtcaaggt cgccgacgtc
     4441 gcgcccgacg ggtcgtcgct gctgctgacg tccggctacc ttcgggcctc gcatcgcgcg
     4501 gtcaacgacc gccgctcgtt gccggccgat ccgtggatcg aaaacacgac caagctgacg
     4561 gtctggccca acgatccgac gctgttccgc atcgacatct ggcccgtcgc cgccgagctt
     4621 cggcccggcc atcggctgcg gatcatgatc tcgtccaccg acgtccccaa ccacgagccg
     4681 ctgccgcacg tgtcccgcaa cacggttcat cattcggccc ggtatccctc ggagctgctg
     4741 ctgacggtgg cgtagcgccg cccgacttgc ccgtttggtt tccccgggtc tacgctcggc
     4801 ggcgacaagt gaatagcgat gtggcggtgc cccgaagctc ggaggccagg ggcgctcaag
     4861 ttccacgagg gggaagccgg tgagagtccg gcgctgtccc gcaactgtaa gcggcgcatc
     4921 gacgccgcaa gccaggatac ccgccgtcac gcgctccggt ctcttcgtgt gaaaggggga
     4981 aggagccaat gcgagacgtc ttcgcattgt ctgcccccca accgtccacc ggttggggtt
     5041 ttcgtttctc gaagcgcatc gcgctcgcga tgctgatggt ggtggcgctg ggcggtgccg
     5101 ttcccgcgca cgccgcgaac gtcaccgtga aggttcgcat cgcggccggc gggcatgccg
     5161 gcgcgatcaa ggactgcgcc gcggtctcgg ttcccgcagg ggcgaacggc ctgacggtgc
     5221 tcggcgccgc ggtcgcgcaa ggctgcatca agtcgttcca gaccgcgagc ggcggggcct
     5281 acgtcacatg catcgacccg ggacaggcat cgctctgcga ggtcgcgggc gggctcgtga
     5341 cgttctgggc cgtctacgac aacgggcggg cctcgacgac gggcatcagc ggcttcgccg
     5401 cgtcgcaagg cagagagctg gcgttcgcct acacgaactt cctgacctgc ttcgcgacgt
     5461 atccgagctg cccgctgtag cgatgagccg gctcctgcgt gcgagcgtcg cggtggcgat
     5521 ggtgatgatc gtcgcgccgt tgtcgctcgc acgcgccgag ccggggcccg cggccgcttc
     5581 ggccgccgcg tgggtcgcgg accggcaagc ggcggacgga ggattcttcg gctccgcgca
     5641 gcccgccgac gcgaccgccg agacgatcgc gtcgctggcg gctgcgggca cgccggccgc
     5701 gcgctcggcg atcggccgcg cgctcgggcg gattcgcgcc gacggaccgt cgcgcgcatc
     5761 gcgtcccgcg tatgcgggcc ggatcgtgat gggcctggtc gccgcgggac gcgatccgcg
     5821 atccttcgac ggaaccaact acgtcgcgcg aatcaccgcc ggctacagcg cggcgtcgag
     5881 cacctacgag accggcgtgt acgcgaacgc gctcgcgatc ctcggtctgg tcgccgcacg
     5941 cgagccggtg ccgtccggcg cggtgaccta catgcgcgcg aaccagtgca gcgacggcgg
     6001 tttcgcgcac gacccggggt gcgtgccgcg cggcgagacc gactcgacct cgatcgtggt
     6061 ctgcgcgctc gccggcatgg gcgtcggtcg cgacgacctg atcgtgtcgc gcgctcgcga
     6121 atggctgcgt gccgtgcgcg cgccgggcgg cggatggccg cactttcccg gcgggccggt
     6181 gaacgccaac tcgaccggtc tggcgctgtc ggctctggcg tgcctgggtg agcgggacgc
     6241 ggcgtcgctt cgcgtgctgc gctcgctgca gatgccgtcg ggcgggatcc gctacgacgg
     6301 cgacgatgcc gagccgaact cgtacgcgac cgtgcagtcg atccccggcc tcgccggccg
     6361 cggctatccg gtgcgccccg caaccgcggt cgcgggcacc aaagaatcgg cggtgccgtc
     6421 cccgccgtcg ccggccgcat cgtcgcgagc gccggccccc gcgggggtgg cggcgccggc
     6481 cgcctcgccc gccggatccg gcagcccgtc gtcgcggccg gccgcggcgg aaaacgggcc
     6541 gcggaacgcg cccgcggctc tgccgccctc gccgcgggac gacgggcgtc ttccacccgc
     6601 ggtgctggcc tccgggctcg cggtcgcggg cgtggccgcc gcgttcgccg gcgcccgcgt
     6661 tcccgccgcc gtgcgccggc ggaggcaccg gtgagggtcg tggccgcagc gatcgtgctc
     6721 gccgtcgcgg ccggcgggtc gaccccggcg accaccccgg cgaccggtcc ggcctcggat
     6781 ccgtgcgagc accgccccgt gcccgtcgcc gccgtgcagt ccggcgagcg gcacgctggc
     6841 ttgatcgtgg agtccccgtc ggggacgaga cagttttgca tcgcgttctc cgcgcaggag
     6901 gaggcggcgg gattcagcgg cgtggatctg ctgcagcgcg ccgagcacga cctcgcgagc
     6961 gtctgccgta tcggcgacga cggatgcggc gatcccggcg actgcttctg ccaatgccac
     7021 gacaccggcg cggcgagctg ccgcttctgg ggctactaca cgctcggcga gcagggcgcg
     7081 tggtcgttcg cgcagcaatc cccgtcgctg cggcgaattc gcgacggcga cgtggacggc
     7141 tggcgcttcg gcgcgcacgc cggcggcgca ggcgagccgc ctgccgtgcc ggaggcgcga
     7201 tgcgcgaacg tagccgtcgc tcgcacggcc ggcggccccg cccggcgcgg cggcggcgtt
     7261 ccgctcggcg tgatcgccgc gagcgtggtc gcgctcggct tcgccgtcgg gatcgcctac
     7321 ggcgttcgcc gccgtccggg aggctcgacg tgacggcgcg gctgcatccg gccgcctgga
     7381 tggtgtggac cgcttcggca tccgccgtga tcctgtcgct ggacaatccc gtcttgatcg
     7441 tgctctcgct cggcgcgctc ggcttcgtcg cgacgtccct cgcgccggac cgcggaccgt
     7501 tcggcctgat gctcaagatc ggcctgatcg cgctcgccgc gcgcaccgtg ctcttcgctc
     7561 tgaccgggca tccgtccggc gagatcctgt tcacgatgcc gcaggtgcgt gtgccggctc
     7621 tgctcggcgg cttcacgctg ggcggaccgg tcaccgcttc ggtcgtggtg cactctgcga
     7681 tcgagggcgc gcggctcgcg gcgttgctgt gctgcttcgg cgtgttcctg tccgcggtcg
     7741 aggccacccg cgtcgtgcgt ctgatgccgc gtttcctgtt cgaggccggg ttgatcgtgg
     7801 cgatcgcgat ctcgttcgtg ccgtcgctgg cgcgtaccgc gcgggacgtg cgcgacgccc
     7861 gccgcattcg cggcgagcgc gtgcgcgggt ggcgcggcac gacgcttgcg atcccgatcc
     7921 tggcgagcac gctcgagcgc gccgtcaccg tcgccgaatc gatggaggcc cgcgggtacg
     7981 ggcgggtcgc gggcggcgac gccggacgcg aggcgttcgc gcggctgcgc acgctcgcgg
     8041 gcgtcacgct gctgaccggg ggagggcttc tggcggcggt cgccggtgtg cgggggggat
     8101 ggatggtgtt cgccgcgggc ggggcgctgg cagcgtcggg cttgcgtgcg ctgtcggggt
     8161 tgtcggcccg gacgcggtac cgccgcgatc ggatgcgagg tgccgacgcc gcggtgacgg
     8221 tcgcggccgt tgcggtcgcg gtggtctcgc tcggcttgcg cggcgcgtgg tcgtacgcgg
     8281 cgtatccgag cgtcaccatg cctgcggttc cggtcgccgg cgtgctcgtc gtcgcgtgcc
     8341 tggcctcgcc cgtggcgatc gtcgcgtggc acgcgcggag cctgcgtcga gcctcgcgcg
     8401 atccggcggg tgccgctgcc gttggcgccg cgcccgtgct gccggccgat ccgctgccgg
     8461 tcgcgggggg gcagtcgtga tccgattcga gggcgtgacc tttacgtatc cgcgcgcggc
     8521 atcgcccgcg ctcgccggcg tggacatgac ctggggccgc gaggaactcg tgctcgtcgc
     8581 cggcgtgtcc ggctcgggca agtcgacgtt gctgcgcgca gcgaacgggc tcgtgcctca
     8641 cttcagcggc ggcacgctcg acgggcgcgt caccgttgcc gggctcgaca cgagatatga
     8701 caagccgcgc gcgctgtccg gagccgtcgg cttcgtcgcg caagatcccg aaggcggatc
     8761 ggtcgcctcg gtcgtcgaag acgagatcgc gttcttcctc gagaacctcg gcctggatcc
     8821 gccgctgatg cgcaagcggg tcgaggagac gctcgacgcc ctcgccatcg cgcatctgcg
     8881 cgagcgcgcg ctggattcgc tctcgggcgg ggagcggcag cgcgtcgcga tcgctgcggt
     8941 gctcgtcgcc gcgccgtcgg tctgcgtgct cgacgaaccc accagcatgc tcgacccgca
     9001 atcggccgag gaggtgctgc agtcggtgct gcggctctcg cacgagatgg ggctcggcat
     9061 cgtgctcagc gagcaccggc tcgagcgggt gctcgcgttc gcggatcgtg tgtgcgtcgt
     9121 cgaggagggc cgggtccgca tcggtccggt acgcgacatg ctcgcggact cgccggtcgc
     9181 tccgcccgtg gtgcggctcg cgcgcgcgct cggctggacg ccggtgccgg tgtcggtgcg
     9241 cgaggcgcgc gctcgcgccg catcgctcga cgtccatccc ggccgagccg cggccggccg
     9301 gcctgcgccc ggcggcgagc gcatcgccgt gaaaaacctt tcggtcgcgc tcggcggacg
     9361 cgaggtggtt cgggatctgg acctttccgt gcgcgaaggc gagacggtcg cggtcgtggg
     9421 ccgcaacggc gcgggcaaga cgacggtgct gcgcgcgatc ctcgggctcg tgcggcgcgt
     9481 gcggggcgcg gtgctgctcg acggcgcgca gcgcgactcc gtcgaggatg cggcaacggt
     9541 cgccgcgtac gtgccgcagg ccgcgtcccg cacgctcacc cgtgcgcgtt cgatcgacga
     9601 gatcgaacgt acgcttcgcc gcaagggcgt gcgcgatccg ctcgcgcccg tccggatctt
     9661 ggacgactgg ggcctgtcgg cgttcgccga gcgcgatccg cgcgacctgt cggccggaga
     9721 ggcgacgcgg ctcgcgctcg ccgccgcgac cgcgcacgac ccgggcatcg tgctgctcga
     9781 cgagcccacg cgcggcatgg acgagcccga gaagcaccgg ctcgcggagc ggctcgccgc
     9841 gtggcgccac gagggccggg ccgtcgtgat ggtcacccac gacgtcgagc tgatcgccga
     9901 agccgcgacg cgcgtcgtgc tgctggcgga cggcgaggcg gtcgtggacg ggcccgcgcg
     9961 ctcggtgctg ggcgagtcgg tgatgttcag ctcgcagatg aacaaggtct tcgaagatcg
    10021 gcggatcgcg acggtcgcgg acgcggtcgc ggcggtgacg ccgtgaccac gatcgccgct
    10081 cccacgggcc gcccgccgcg cgcttcgcgc gccgcgacgg tggcatcccg cgcgctgctg
    10141 atcgcggcga acgccgccgc ggtcgccgcg ctgctcgcac ccatgatcgc gggagccgac
    10201 cccgccgccg gccgcgaggc cgcgcacgcg accgacgggc ccatcctgct cgcgctggcg
    10261 gtaccggcgc tgctggccgt cgcgctgggc gaggcgagcg gcaagcggct cgatgcgaaa
    10321 ggcatcgcgc tgctgggcgt gctgt
//
LOCUS       DATLFO010000077         9906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_110982, whole genome shotgun sequence.
ACCESSION   DATLFO010000077 DATLFO010000000
VERSION     DATLFO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110982"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VM841_02665"
     CDS             <1..819
                     /locus_tag="VM841_02665"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HVL89118.1"
                     /translation="VEVGRRLAEALDARGVRVSATPEDAARLKDSVTPASDADLDAGF
                     DLMFALGGDGTFLHAAQIAIRSGTPLLGVNLGRIGFLSALERHSLDDALVDTIIGQDL
                     PITERLTVEGEIVVAGEVKHRLWALNDIAVSKLEPGRLIKLRLAIDNEPFADLSADGI
                     IVSTPTGSTAYSFSAGGPIVSPAVDCLIVTPVSPHLFFDRSIVAGPGEEVQITILPDP
                     DSVGVSPDGRRWVEAGFGATVLVRPSRTRLRLAVVEPAPFWRLVRTKFGLPTPD"
     gene            849..2012
                     /gene="steA"
                     /locus_tag="VM841_02670"
     CDS             849..2012
                     /gene="steA"
                     /locus_tag="VM841_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HVL89119.1"
                     /translation="MRRKMRRRPDTIAGVARTDRRTKELIQRIRPGEIAVIDHEDLDR
                     VSAEGLVEAGVALVVNARSSITGRYPNTGPLVLARAGIPIVDGCGPALFEVVHEGDEI
                     RTEEGRIYRGPAAVIEGVLLDEDEIVRRIAHAEASVGEELERFVVNTMDYLQTEGDMI
                     LRGEGIPFVRTDFRGRHALIVVRGHDFKQDLRTLRGYISDLKPVLVAVDGAADALLDE
                     GWKPDMIIGDMDSVSERALRSGADLVVHGYPDGRAPGLERLASLGLGGTVFATGGTSE
                     DIAMLLAYERGADLIVAVGTHANLVEFLDKGRAGMASTFLVRLKVGPKLVDAKGVNRL
                     YRNEISRGLLILLVASAFLAMVVAFSAAPGLRLFIENLGERFQDLWFRIRHLF"
     gene            2016..2951
                     /locus_tag="VM841_02675"
     CDS             2016..2951
                     /locus_tag="VM841_02675"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HVL89120.1"
                     /translation="MINFRYHVVSIVAVFLALAVGIVMGTTVISESVVDRLEADARDF
                     RARNGQLRQEADRLEAELGQYEQFGAAIVPPLIRDRLEGHPVAVLVEPGVPGALLDRT
                     TDTLRTAGASPGGTIRLTEAWTLEDPAHREQLAAVLGADANPSDGPTLLSRGAEALGR
                     RLGSDSDGGDDDLLRALDRAGFVKLDDLPAGALPGASTLIVYLSASAATAAPNDETFT
                     IPLLDMLAERSAATPVVVAEPLDADESVVDRIRGIDELARAIATVDHATTWPGALSLV
                     WALARGAGERVTHYGVRRGAAAIAPAVTPAPAASS"
     gene            2948..3649
                     /locus_tag="VM841_02680"
     CDS             2948..3649
                     /locus_tag="VM841_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL89121.1"
                     /translation="MTRVVAIVPAYQEAGRVAPAVTALKEFCDRIIVVDDGSTDATAD
                     EAAAAGARVIRSSRNRGKGSALAAGIRAAGADAQVLLLADADLASSASTLRALLRPVV
                     DGEADLTIAAPPPSGPSGFGLVESLARHGIKALARLDTTRPLSGQRAIRADLASRLRL
                     DRRFGVETGMTIDAVRLGARVVEVPCAFTHARTGRDLAGFAHRAKQGLGVGRAVLRRL
                     PGLLRRPPKRGNVAS"
     gene            3655..4551
                     /locus_tag="VM841_02685"
     CDS             3655..4551
                     /locus_tag="VM841_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89122.1"
                     /translation="MRLVVVNVLAVAVAAAATRFAMQRLIPNAPLRRSNYRGVPVATG
                     LGIAVFAGFVVACGVLALLSEISAGWVAPGVWILPLAAGGVFGLLGLFDDAASALTPD
                     ADKGLRGHTHALSGGRLTSGALKMVAGSAAGFALAAPATEGFAFALAGGAIIAMSAHV
                     MNAFDLRPGRAAKLFVAGAIPLAIVAGPLRAVVFAAIGAVIAALPDDLRERAMLGDAG
                     AGALGAIAGSTALLISTRTTLLITLAVLAVLTVIAEGPTISRMIERVPFLRAADRWGR
                     VPEPTRYPQPPASISGTADDNS"
     gene            4635..6266
                     /locus_tag="VM841_02690"
     CDS             4635..6266
                     /locus_tag="VM841_02690"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVL89123.1"
                     /translation="MAKHIFVTGGVSSSLGKGLTASSLGRLLKSRGLRVVMQKLDPYI
                     NVDPGTMNPFQHGEVFVTNDGAETDLDLGHYERFIDADLSRDSNVTTGSIYSTVIARE
                     RRGEFLGATVQVIPHITEEIKSRIRRLVNEDVDIVITEVGGTVGDIESLPFLEAIRQF
                     RHEVGRSNVIFIHVSLVPYIGPSGELKTKPTQHSVAELRSIGIQPDVIVCRSDRPLST
                     GLKKKISQFCDVDLDAVVSAMDARSIYEIPLVLHEEGLDATVVKHLGLDAPEPDLSEW
                     EALVRRINTAKHTVKIAVCGKYVTLADSYLSVVEAVRHGAIANDATAEIVWVASDELT
                     DPAKAQELLGDVDGILVPGGFGVRGIEGKIAAVRYARENQIPFLGLCLGLQCAVIEYA
                     RNVVGLENANSSEFDAASPHPVIDLMPDQKDVLNKGGTMRLGLWPCKVAPGTKAHAAY
                     GENLVLERHRHRYEVNNNYRKQLANAGLVFSGTTVDDRLVEMVELADHPWFVASQFHP
                     EFKSRPTRAHPLFRDFAAAALKRVSSEQSHADTNA"
     gene            6300..7418
                     /gene="ald"
                     /locus_tag="VM841_02695"
     CDS             6300..7418
                     /gene="ald"
                     /locus_tag="VM841_02695"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HVL89124.1"
                     /translation="MIVGVPKEVMEAEYRVAITPVGVRELVREGHHVVIEQTAGDGSS
                     IYDDDFVRAGARILTTADDVWGAADMILKVKQPLPEEFPRMRRNQLLFTYLHLAAHKD
                     VTEALVQSGTTGIAYETVELPDRSLPLLAPMSEIAGRMAPHVAANYLHRANGGRGVLM
                     SGVSGVRPARVLVIGAGMSGMNAAWIAQGLEAEVILLDKVVEKLRYVDHIHKGRILTL
                     ASAALTIEEEVMAADVVIGAVLVAGARAPMLISEDLVRAMKPGAVVVDISIDQGGCLE
                     TSRVTTHTNPTYVTHGVVHYCVGNMPGAVPHTSTYALANATMPYALALARTGLNALVA
                     DPALAKGVNVHDGRVTNEAVAAAVGMSWTPLAALLPGA"
     gene            7495..8454
                     /gene="xerD"
                     /locus_tag="VM841_02700"
     CDS             7495..8454
                     /gene="xerD"
                     /locus_tag="VM841_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HVL89125.1"
                     /translation="MTRLTERYLDHLAVERGVAPNTVAAYRRDLRLYLTDLGERGIDD
                     PAAVGEEVVGAFLERLRESEYAPGKRYSAATVARVLAAVRGFHRYLLREEVTKLDPAW
                     MIGSPRTPRALPKALDQDEVEALLAAVPEEGAQGARDRALLETLYAAGLRISELTGLD
                     VDDVDLDERTVRCLGKGSKERIVPIGRPATEALRHYLTQARPGFVRGRSESALFVNAR
                     GRRLTRQGCWKLLKKYAERASLQRRISPHTLRHSFASHLIEHGADVRDVQELLGHASV
                     STTAVYTLLHRDKIREIYDRAHPRARRATPMSESIGEHAGDAS"
     gene            8467..8814
                     /locus_tag="VM841_02705"
     CDS             8467..8814
                     /locus_tag="VM841_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HVL89126.1"
                     /translation="METAQLEKFRELINGEREDVRKQLADLGADPDAESFENTDFDFG
                     FADSAQSTAERNRVLVVIEGLRERLIDINAALDKIDKGTFGLCENCGNEIGAERLEAL
                     PHTRLCTNCKSKG"
     gene            8952..9695
                     /locus_tag="VM841_02710"
                     /pseudo
     CDS             8952..9695
                     /locus_tag="VM841_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
BASE COUNT         1582 a         3473 c         3326 g         1525 t
ORIGIN      
        1 gtcgaggtcg gccgccgcct ggccgaggcg ctcgacgccc gcggcgtgcg cgtcagcgcg
       61 accccggaag acgccgcgcg tctcaaggac agcgtcaccc ccgcatcgga cgccgatctc
      121 gacgccggct tcgacctgat gttcgcgctc ggcggcgacg gcacgtttct gcacgccgcc
      181 cagatcgcga tccgctcggg cactccgctg ctgggcgtga acctcggccg catcgggttc
      241 ctgtcggcgc tcgaacggca ctcgctcgac gacgcgctgg tcgacacgat catcggccaa
      301 gacctgccga tcaccgagcg actcaccgtc gaaggcgaga tcgtcgtcgc gggcgaggtc
      361 aagcatcggc tctgggccct caacgacatc gccgtctcca agctcgagcc cggccgcctc
      421 atcaagctgc ggctcgcgat cgacaacgag ccgttcgcgg atctgtccgc cgacggcatc
      481 atcgtgtcga ccccgaccgg ctcgaccgcc tactccttct cggccggcgg gccgatcgtc
      541 agccccgccg tggactgcct gatcgtcacg cccgtctcac cgcacctgtt cttcgaccgg
      601 tcgatcgtgg ccggaccggg tgaagaggtg cagatcacca tccttccgga tcccgacagc
      661 gtcggtgtct cgcccgacgg ccgcagatgg gtcgaagccg ggttcggtgc gacggtgctg
      721 gtgcggccgt cccgaacgcg gctccggctc gccgtcgtcg agcccgcccc gttctggcgt
      781 ctggtccgga cgaagttcgg cctgccgacg cccgattgag cgcccgggcg ccggataaga
      841 tcggccgcat gcggcgcaag atgcgacggc ggcccgacac gatcgccggt gtcgcgcgca
      901 ccgatcgccg gacgaaagag ctcatccaac gcatccgccc gggcgagatc gcggtcatcg
      961 accacgagga cctcgaccgc gtctctgccg aaggcctcgt ggaggccggc gtggcgctcg
     1021 tggtgaacgc gcgctcgtcg atcacgggcc gctacccgaa caccggcccc ctggtgctcg
     1081 cgcgcgccgg catcccgatc gtggacggct gcgggcccgc gctcttcgag gtcgtgcacg
     1141 aaggcgacga aatcaggacc gaggaaggcc gcatctaccg cggccctgcc gccgtgatcg
     1201 agggcgtgct gctcgacgag gacgagatcg tccgccgcat cgcgcacgcc gaggccagcg
     1261 tcggcgagga gctcgaacgg ttcgtcgtca acacgatgga ctacctgcag accgaaggcg
     1321 acatgatcct gcgcggcgaa ggcattccct tcgtccgcac cgacttccgc gggcgtcacg
     1381 cgctgatcgt ggtgcgcggt cacgacttca agcaggatct gcgcacgctc cgcggctaca
     1441 tctccgactt gaagccggtg ctggtcgccg tcgacggcgc cgccgacgcc ctgctcgacg
     1501 agggctggaa gcccgacatg atcatcggag acatggactc ggtaagcgag cgcgcgctgc
     1561 gctcgggcgc cgacctcgtc gtgcacggct accccgacgg ccgcgcccct gggctggagc
     1621 gcctggcctc gctcgggctc ggcggcacgg tcttcgccac cggcggcacc agcgaggaca
     1681 tcgcgatgct cctggcttac gagcgcggcg ccgacctgat cgtggcggtg ggcacgcacg
     1741 cgaacctggt ggagttcctc gacaagggcc gggccggcat ggcctcgacg ttcctcgtcc
     1801 ggctcaaggt cgggcccaag ctcgttgacg cgaagggcgt gaatcgcctg taccgtaacg
     1861 agatctcgcg cgggcttctg atcctgctcg tggcgagtgc gttcctggcg atggtggtgg
     1921 cgttctcggc cgcgccgggg ctacgactgt tcatcgagaa cctgggggag cggttccagg
     1981 atctgtggtt ccggatccgg cacctgttct agcgcatgat caactttcgt tatcacgtcg
     2041 tgtcgatcgt cgcggtgttc ctcgcgctcg cggtcggcat cgtcatgggc acgacggtca
     2101 tcagcgaaag cgtcgtggac cgcctcgagg ccgacgcgcg cgattttcga gcccgcaacg
     2161 gccagctccg tcaagaagcc gaccggctcg aagccgagct cggccagtac gagcagttcg
     2221 gcgccgcgat cgtgccgcct ctgattcgcg accggctgga aggacatccg gtggcggtgt
     2281 tggtcgaacc gggggtgccc ggcgcgctgt tggaccgcac gaccgacacg ctgcgcaccg
     2341 cgggagcgtc cccgggcggc accatccggc tgaccgaggc gtggacgctg gaggatccgg
     2401 cgcatcgcga gcagctcgcc gcggttttgg gcgccgacgc caacccctcc gacggcccga
     2461 ccctgttgag ccggggggcc gaggcgctcg gacggcgcct gggcagcgac tccgacggcg
     2521 gcgacgacga cctgctgcgc gcgctggacc gagcggggtt cgtcaagctc gacgacctgc
     2581 ccgccggcgc gcttcccggc gccagcacct tgatcgtgta tctgtcggcg tccgccgcga
     2641 ccgcagcccc caacgacgag accttcacga tcccactgct ggacatgctc gcggagcgat
     2701 cggccgccac gccagtcgtc gtggccgaac cgctcgacgc agacgaatcg gtggtggacc
     2761 ggatccgcgg catcgacgag ctcgcgcgcg ccatcgcgac cgtcgatcac gcgacgacct
     2821 ggcccggggc gctgtcgctc gtatgggccc tcgcacgcgg cgccggcgag cgcgtcacgc
     2881 actacggcgt gcgccgggga gcggctgcga tcgcgccggc ggtcacgccc gcgccggctg
     2941 cctcatcgtg acccgcgtcg tcgccatcgt gcccgcctat caagaagcgg gccgggtcgc
     3001 gccggccgtg acggccctca aggagttctg cgatcggatc atcgtcgtcg acgacggatc
     3061 caccgacgcg accgccgacg aggccgcggc cgcgggagcg cgcgtgatcc gttcgagccg
     3121 caaccgcggc aagggctcgg cgctcgccgc cggcatccgc gcggccggcg cggacgcaca
     3181 agtactgctg ctcgccgacg cggatcttgc gtcgtcggcg tccacgttgc gcgcgctgct
     3241 gaggccggtc gtggacgggg aggcggatct cacgatcgcg gccccaccgc catcgggccc
     3301 ctcgggcttc gggctggtcg agagcctcgc gcgtcacggc atcaaggcgc tcgcgcggct
     3361 cgacacgacc cggcccctgt cgggtcagcg ggcgatccgc gcggacctcg cgagccgcct
     3421 gaggctcgat cgccggttcg gcgtcgagac cgggatgacc atcgacgcgg tgcgtctggg
     3481 cgcgcgggtg gtcgaggtcc cgtgcgcgtt cacccacgcc cgcaccggac gcgacctcgc
     3541 gggcttcgct caccgggcca agcaggggct cggcgtcggc cgcgccgtgc ttcggagact
     3601 gccggggttg ttgcgccggc ccccgaagcg cggcaacgtg gcatcgtgag catcgtgagg
     3661 ctcgttgtgg tcaacgtcct ggcggtggcc gtcgcagccg ccgccacacg cttcgcgatg
     3721 cagcggctga tccccaacgc gccgctgcgc cgatccaact atcgcggcgt ccccgtggcg
     3781 accgggctgg gcatcgccgt gttcgcagga ttcgtcgtcg cgtgcggcgt gctggcgctg
     3841 ctcagcgaga tcagcgccgg gtgggtcgct ccgggtgtgt ggatcctgcc gctcgcggcc
     3901 ggcggcgtct tcgggctgct cgggctcttc gacgacgcgg cgagcgcgct caccccggac
     3961 gccgacaaag gcttgcgcgg gcacacgcac gcgctctccg gcggacggct gaccagcggc
     4021 gcgctgaaga tggtcgccgg atcggccgcc gggttcgccc tggccgcgcc cgccaccgaa
     4081 ggcttcgcgt tcgcactcgc aggcggtgcg atcatcgcga tgtcggccca cgtcatgaac
     4141 gctttcgatc tgcgccccgg ccgcgccgcc aagctgttcg tcgcgggcgc gatcccactg
     4201 gcaatcgtcg cgggaccgct ccgcgccgtc gtgttcgccg caatcggcgc cgtcatcgcc
     4261 gcgctgcccg acgacctgcg tgagcgagcg atgctcggtg atgcgggcgc cggcgcgctc
     4321 ggggcgatcg cgggctcgac cgcgctgctc atctcgaccc gcactacgct gctgatcacc
     4381 ctggccgtgc tcgcggtgct cacggtgatc gcggaaggac cgacgatctc tcgcatgatc
     4441 gagcgcgttc cgtttctgcg ggccgccgac cgatgggggc gcgtgccgga acccacacgt
     4501 tatccacagc ccccggcaag catttcgggc accgccgacg acaactcgta gcgcaacact
     4561 gctggatacg cgacccgcgc cctgctagga tgaggtcccg agggccactg cggcccgcgg
     4621 gagatttttt ttctatggcc aagcacatct tcgtcaccgg cggcgtttct tcttccctcg
     4681 gcaaaggcct cacggcatcg tccctcggtc gtcttctgaa atcgcgcggc ctgcgcgtcg
     4741 tcatgcagaa gctcgatccc tacatcaacg tcgatcccgg aacgatgaac ccgttccagc
     4801 acggggaggt cttcgtcacc aacgacggcg ccgagacgga tctggacctg ggccactacg
     4861 agcgtttcat cgacgccgac ctgtcgcgcg actccaacgt cacgacggga agcatctact
     4921 cgaccgtcat cgcgcgtgaa cggcgtggcg aattcctcgg cgcgaccgtg caggtcatcc
     4981 cgcacatcac cgaagagatc aagagccgca ttcgccgcct ggtcaacgaa gacgtcgata
     5041 tcgtcattac cgaggtgggc ggcaccgtcg gcgacatcga atcccttccg ttcctggagg
     5101 cgatccgtca gttccgccac gaggtcggcc gctcgaacgt gatcttcatc cacgtgtcgc
     5161 tcgtgcccta catcggcccg agcggggagc tcaagaccaa gccgacgcag cacagcgtcg
     5221 cagagcttcg ctcgatcggt atccagccgg acgtgatcgt gtgccgcagc gaccgtccgc
     5281 tgtccacggg cttgaagaag aagatcagcc agttctgtga cgtggacctg gacgcggtcg
     5341 tgtccgcgat ggacgcgcgc agcatctacg agattccgct ggtcctgcac gaagagggtc
     5401 tcgacgccac ggtcgtgaag catctcggcc tggacgcgcc cgagcccgac ctgagcgagt
     5461 gggaagcgct ggtgcgccgc atcaacaccg caaagcacac ggtcaagatc gcggtgtgcg
     5521 gcaagtacgt gacgctcgcc gactcgtacc tgtcggtcgt cgaagcggtg cggcacggcg
     5581 cgatcgccaa cgacgcgacc gccgagatcg tctgggttgc atccgacgag ctcaccgatc
     5641 ccgcgaaggc gcaggagctg ctcggcgacg tggacggcat cctggtgccc ggcggcttcg
     5701 gcgtgcgcgg catcgaaggc aagatcgccg cggtgcgcta cgcgcgcgag aaccagattc
     5761 cgttcctcgg tttgtgcctg ggtctgcagt gcgcggtgat cgagtatgcg cgcaacgtcg
     5821 tcggcttgga gaacgcgaac tcctccgagt tcgacgccgc ctcgccgcat ccggttatcg
     5881 acctgatgcc cgaccagaag gacgtgctca acaagggcgg cacgatgcgg ctgggcctgt
     5941 ggccgtgcaa ggtcgcgccc ggcaccaaag cgcacgccgc ctacggagag aacctggtcc
     6001 tggagcgtca ccgccatcgc tacgaggtca acaacaacta ccgcaagcag ctcgcgaacg
     6061 cgggcctggt cttcagcggc accaccgtcg acgaccgcct cgtcgagatg gtcgagcttg
     6121 ccgaccaccc ctggttcgtc gccagccagt tccatcccga gttcaagagc cgccccacgc
     6181 gcgcccaccc gctcttccgc gacttcgccg ccgcggccct gaagcgagtt tcctcggaac
     6241 agtcccacgc cgacaccaac gcatagaatc cgacccgggc gtacacgaag ggggcgcggg
     6301 tgatcgtcgg agttccgaag gaagtgatgg aggccgaata tcgggtcgcc atcacgccgg
     6361 tcggggtacg cgagctggtt cgcgaaggcc accacgtcgt catcgagcag acggcaggcg
     6421 acggatcgag catctacgac gacgacttcg tgcgcgccgg cgcgcgcatc ctgaccacgg
     6481 ccgacgacgt ctggggcgca gccgacatga tcctgaaggt caagcagccg ctgcccgaag
     6541 agtttccgcg gatgcgcaga aaccagctgc ttttcacgta tctgcatctg gccgcgcaca
     6601 aggacgtgac cgaggcgctg gtccagtcgg gcacgaccgg catcgcctac gaaaccgtcg
     6661 agcttcccga ccgctcgctg ccgctgctgg ccccgatgag cgagatcgcc ggccgcatgg
     6721 ccccgcatgt cgcggcgaac tatctgcatc gcgccaacgg cggccgcggc gtgctgatga
     6781 gcggcgtgtc gggcgtacgg cccgcgcgcg tgctggtgat cggcgcaggc atgagcggca
     6841 tgaacgccgc ctggatcgcg cagggtctgg aagccgaggt gatcttgctc gacaaggtgg
     6901 tcgagaagct tcgctacgtg gatcacatcc acaaaggacg gatcctcacg cttgcttcgg
     6961 ccgcgctcac gatcgaagag gaggtcatgg ccgccgatgt cgtgatcggc gcggtcctgg
     7021 tcgcgggcgc gagggcgccg atgctgatct cagaggatct ggtgcgggca atgaagccgg
     7081 gcgcggtggt tgtcgacatc tcgatcgacc agggcggatg cctcgaaacg agccgagtca
     7141 ccacgcacac caatccgact tatgtcaccc acggggtggt acactactgc gtaggaaaca
     7201 tgccgggtgc cgtcccgcat acgtccacct acgctcttgc gaacgcgacg atgccgtacg
     7261 cgctcgcgct ggcgcgaacc ggactgaatg cgctggtcgc ggatccggct cttgccaagg
     7321 gagtcaacgt gcacgacgga cgagtgacga acgaagcggt cgccgccgcc gtcggcatgt
     7381 cttggacacc gctggcagct ctcttgcccg gcgcctgacc tcatccggct gccggccctc
     7441 tcttcttagg agacctcgct gacgacgaac ccgaaagcgg atccgcctcg cgcaatgacg
     7501 cgcctgacgg agcgctacct cgaccatctc gcggtcgagc ggggcgtcgc ccccaacacc
     7561 gtcgccgcgt accggcgcga ccttcggctg tacctgacgg atctgggaga gcggggcatc
     7621 gacgatccgg ccgcggtcgg ggaggaggtg gtcggcgcgt tcctggagcg tctgcgagaa
     7681 agcgagtatg cgcccggcaa gcgctactcg gccgcaacgg tcgcgcgtgt gctcgccgcc
     7741 gtccggggat tccatcgcta cttgctccgc gaagaggtca ccaagctcga cccggcatgg
     7801 atgatcggct cgccgcgcac cccgcgggcg cttcctaagg cgctggacca ggacgaggtc
     7861 gaggcgctgc tcgcggccgt cccggaggaa ggcgcgcagg gcgcgcgcga ccgggcgctg
     7921 ctcgagaccc tctacgcggc cgggcttcgc atcagcgagc tgaccgggct ggacgtggac
     7981 gacgtcgacc tggatgagcg cacggtccgt tgcctgggca agggctcgaa ggagcggatc
     8041 gtgccgatcg gccgtccggc gaccgaggcg ctgcgccact acctgacgca agcgcggccc
     8101 gggttcgtgc gcggccgcag cgagtccgcc ttgttcgtca acgcgcgggg ccgccgcctg
     8161 acccgtcagg gctgctggaa gcttttgaag aagtacgccg agcgcgcatc gttgcagcgt
     8221 cgcatctcac cgcacacgct ccggcattcg ttcgcgtcgc acctgatcga gcacggcgcc
     8281 gacgtccgcg acgttcaaga gctgctcggc catgcgagcg tgtccacaac cgccgtctac
     8341 acgctccttc accgcgacaa gatccgcgag atctacgacc gagcccatcc gcgcgcgcgg
     8401 cgcgcgacac ccatgagcga atcgatcggc gaacacgccg gcgacgcctc ctaggagggt
     8461 ccaccgatgg aaaccgccca gctcgagaag ttccgcgaac tcattaacgg cgaacgcgaa
     8521 gacgtccgga agcagctcgc cgatctcggc gccgatcccg acgccgaatc gttcgagaac
     8581 accgacttcg acttcgggtt cgccgactcg gcccagtcca cggccgagcg caatcgagtg
     8641 ctggtcgtca tcgagggcct ccgggagcgg ctcatcgaca tcaacgccgc cctcgacaag
     8701 atcgataagg gcacgttcgg tctctgcgag aactgcggaa acgagatcgg cgccgaacgc
     8761 ctggaagcct tgccccacac gcggctgtgc accaactgca agtccaaagg ctgacagcga
     8821 cgcggcgaag ttttccttgc ccgtggcgta cgggctccga tagcatccca cccctgtggc
     8881 gactcgtcgc catgtcgaca attgcgccgc cctgcgaccg ggcgtgcgaa gggggagtgt
     8941 ggatggcgcg ggtcatcagt ctcgccaacc agaagggcgg cgtcggcaag accaccacca
     9001 cgatcagcct cggcgcggcc atggccgagc gagggctgcg cgtcctgctg gtggacttcg
     9061 atccgcaggg gtcgatgtcg gtcggcgtcg gagtcaactc cccggatctc gatctgacga
     9121 tctacaacct gctgctcgac cgctcgatcg atccggcgac cgcgatctgc aagacctcga
     9181 cggacggcat ggagctcctc cccgcgaaca tcgatctgtc cgcggccgag gtcgtgctcg
     9241 tcagcgaggt cgcgcgcgaa caggcgctca agcgcgtgct ggcaaaggtc aaggacggct
     9301 acgactacat tctgatcgac tgcccgccat cgctgggtct gctcacgatt aacgcgctga
     9361 cggcgtccga cggcgtgatc atcccgctgg agtgcgagtt cttcgcgcta cgcggcatga
     9421 gcctgctgat cgacacgatc gaaaaggtca gggaccgttt gaaccctgag cttgaacttc
     9481 agggaatcgt cgcaaccatg tacgacggcc gaaccgtcca ctcgagggaa gtgctcgacc
     9541 gggtgaggca gaagttcggc tcgaagcttt tcaacagcgt cgtgaccagg acgatcaggt
     9601 tcgccgaggc gcctgtagcc ggcgagccga tcttgtccta cgcctccgac tccaagggag
     9661 cgcaggccta tcgagatcta gccaaggagg tgctcggcat tgacgaccaa gagagcgagc
     9721 ctgccgggag cggacgagct gtttcgaaac acggcgagcc cggcgaccag cgagaaggag
     9781 ccggaaacat cggaggtggc cccggtcacg ccgatgccgc agcccccagc ctcttcgacc
     9841 ccggccccgc agcccgtcca agccccgccg gcgcagagcc cgcctccgat ccaagcgacc
     9901 ccggcc
//
LOCUS       DATLFO010000078         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_81110, whole genome shotgun sequence.
ACCESSION   DATLFO010000078 DATLFO010000000
VERSION     DATLFO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81110"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VM841_02715"
     CDS             complement(<1..440)
                     /locus_tag="VM841_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943786.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HVL89127.1"
                     /translation="MNKRYFIRTFGCQMNEHDSERIAGMLEAEGYVRAASAEQADLVL
                     LNTCAIRENADNKLYGALGQLKPAKDANPHLRIAVGGCLVQKDKGLVIEKAPWVDVAF
                     GTFAVAELPDLLRRREQTGETAFEFREQTEIFPSALPARREHPF"
     gene            complement(553..>3097)
                     /locus_tag="VM841_02720"
     CDS             complement(553..>3097)
                     /locus_tag="VM841_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89128.1"
                     /translation="LAPLPAGSATGTVNSVPVLRPSGAPFGGGTVEALRFGEAALNLG
                     AIFGPTFPGCPGFAQFWVKTRASGESFNSQLKDRGKPVPLTLARCPDVHVTKSPQNQT
                     LDVGETVSFTLVATNDGIAPATGVVVTDNLLNVLTDVSAIYDVDPGSPGGTGTCSIRS
                     PILVRCEIGTLAASDGNTTGAEPDTVVVTITATTTPASCGERGNKAHVEADAEAEADT
                     GDNDSNRVSFTVRCADVAIIKTPDALEVNAGSPAGFTIEATNQGGGTATGVTITDVLP
                     AGPGLDWSVDAVSSGMTCGIAAGTLTCTQPALAPGASIRVHMTTPTAVGSCGTIDNTA
                     SVTTTNDGGDEAHASIRVLCPSLGIEKTADDEVVTAGESIGFTISVSNDGAGTATSVT
                     LSDTLPAGPGLGWAVDGGSAAGRCAIEDGVLSCDLGDLAAGAGAEVHISSRTSGDSCG
                     SYENLARASAANHGEVAARATTRVDCAAIEILKTADEISVEAGGPAGFTIEVRNGGDG
                     IARGVVVRDPLPANPGLSWTIDGGSAAGACTIAEGVLGCDLGTLAPGEAATVHIASPT
                     DGRSCGPIVNVAAVNTSNDGSALSSATIVVDCRIGIGIVKEGPDLAHRGDVIEYTMAV
                     TTSTLTPLAEVTVTDPLCDAAPLLRFGDDGDGLLEVGETWMFTCTHVVASGSPDPLPN
                     TAVASGVGNRSPVTASDDHVVDLIAPAIAIDKTVTPVSGTTGTPVTYTYVVTNTGDTT
                     LFDVEVNDDVLGPIGTVAVLAPGSSETFTRASVLGAAAITNIGIASGEDVLGLRVTAI
                     DPATTTVVAGIRLVRTGSGTAGWLAGALLVAAGSVMIGAGRRRGKRAEA"
BASE COUNT          445 a         1084 c         1063 g          505 t
ORIGIN      
        1 tggaacggat gctcacggcg ggccggcagg gcgctcggaa agatctcggt ctgctcgcgg
       61 aactcgaagg cggtctcgcc ggtttgctcg cgccggcgca gcaggtccgg cagttccgcg
      121 accgcgaagg ttccgaaggc cacgtcaacc cacggggctt tttcgatcac caagcccttg
      181 tccttctgaa ccaggcaccc gccgaccgcg atgcgaagat gcgggttggc gtccttggca
      241 ggcttgagct gcccgagcgc gccgtagagc ttgttgtccg cgttctcgcg gatcgcgcag
      301 gtgttgagca gcacgaggtc ggcttgctcg gcgctggccg cgcgcacgta gccctcggcc
      361 tcgagcatgc cggcgatgcg ctccgagtcg tgctcgttca tctggcaacc gaaggttcga
      421 atgaaatagc gcttgttcac ggcggccatg ctaccgcgcc ccggcgagcc cttctcgcga
      481 aagcgccgga tgaagcttcc cgcgagggaa gccgggagcc cgcggggctc ccggcttccg
      541 ttcgcggcgt ttctaggcct ctgcccgttt gcctcggcgc cgccctgcgc cgatcatgac
      601 cgacccggcc gccaccagca gcgctccggc aagccacccg gccgtgccgc tgccggtccg
      661 caccagccgt atcccggcca ccacggtcgt cgtcgccgga tcgatggccg ttacgcgtag
      721 ccccagaacg tcctcacccg aggcgatgcc gatgttggtg atcgccgccg ccccgagcac
      781 cgacgcgcgc gtgaacgtct cgctcgatcc cggcgccagc accgcgaccg tcccgatggg
      841 cccgaggacg tcatcgttga cctccacgtc gaacagggtg gtgtcaccgg tgttggtcac
      901 gacgtaggtg taggtaaccg gggtcccggt cgtaccggag accggcgtga cggtcttgtc
      961 gatggcgatc gccggcgcga tcaggtcgac gacgtgatcg tcgctcgccg tcaccgggga
     1021 ccggtttccc acgcccgagg ccaccgccgt gttcggaagc ggatcgggcg agccggacgc
     1081 gacgacgtgg gtacaggtga acatccacgt ctcgcccact tcgagcaggc cgtccccgtc
     1141 gtcaccgaac cgcagcagcg gcgcggcgtc gcacaacgga tcggtgacgg tcacctcggc
     1201 gaggggcgta agcgtcgagg tcgtcaccgc catcgtgtac tcgatcacat ccccgcggtg
     1261 ggcgaggtcg gggccttcct tgacgattcc gatgccgatg cgacagtcca ccacgatggt
     1321 ggcgctgctc agcgccgaac cgtcgttgct cgtgttcacc gccgccacgt tgacgatcgg
     1381 tccgcacgag cgcccgtccg tcggcgacgc gatatggacc gtggcagcct ctcccggagc
     1441 gagcgtcccc agatcgcagc cgagcacgcc ctcggcgatc gtgcaggccc cggccgccga
     1501 tccaccgtcg atcgtccagg acaagccggg attggccggc agcgggtccc ggacgacgac
     1561 tccgcgcgcg atgccgtcgc ccccgttgcg gacttcgatg gtgaagccgg ccgggccgcc
     1621 ggcctcgacc gagatttcgt cggcggtctt gaggatctcg atcgcggcgc agtccacgcg
     1681 ggtcgttgca cgcgcggcga cctcgccgtg gttggccgcg gatgcgcgcg cgaggttttc
     1741 gtagcttccg cacgaatcgc cgctcgtccg cgaggagatg tgaacctccg cgccggcgcc
     1801 ggcggccagg tcgcccagat cgcacgagag cacgccatcc tcgatggcgc atcgaccggc
     1861 cgccgatccg ccgtcgacgg cccagccgag gccgggtccg gccggcagcg tgtccgaaag
     1921 cgtcacggac gtggccgtac cggcgccgtc gttgcttacc gagatcgtga atccgatcga
     1981 ctcgccggcc gtgacgacct cgtcgtcggc ggtcttttcg atcccaagcg atgggcacag
     2041 gacccggatc gaagcgtgcg cttcgtcccc gccgtcgttg gtggttgtca ccgacgccgt
     2101 gttgtcgatc gtgccgcacg agccgacggc ggtgggcgtc gtcatgtgca ccctgatgct
     2161 cgcgccgggt gcgagtgccg gctgcgtgca cgtgagcgtg cctgccgcga tgccgcaggt
     2221 catcccggac gagacggcat cgaccgacca gtcgagtccg gggccggccg gaagcacgtc
     2281 cgtgatcgtc acgcccgtgg cggtgccccc gccctggttg gtggcctcga tcgtgaagcc
     2341 cgccggcgag cccgcgttca cctcgagagc gtcgggcgtc ttgatgatgg ccacgtcggc
     2401 gcagcgaacg gtaaagctca cccgattcga gtcgttgtcg ccggtgtccg cctcggcctc
     2461 ggcatcggct tcgacgtgcg ccttgttgcc gcgctcgccg cacgatgcgg gcgtcgtggt
     2521 cgcagtgatc gtgaccacga cggtgtcggg ctcggcgcct gtggtgtttc cgtcggacgc
     2581 cgcgagcgtg ccgatctcgc atcgcaccag gatggggctc ctgatcgagc aggtcccggt
     2641 cccgcccggt gatccggggt cgacatcgta gatcgcgctg acgtcggtca acacgttcag
     2701 caggttgtcg gtgacgacca cgccggttgc gggcgcgatg ccgtcattgg tggccacgag
     2761 cgtgaacgac acggtctcgc ccacgtccag ggtctggttc tgcgggctct tggtcacgtg
     2821 cacatcgggg catcgcgcca aagtgagggg gaccggcttg ccgcgatcct tgagctgcga
     2881 gttgaacgac tcgccggagg cacgggtctt gacccaaaac tgcgcgaatc ccgggcatcc
     2941 ggggaacgtc ggcccgaaga tcgcacccag gttcaatgcg gcctcaccga aacgcagcgc
     3001 ctccaccgtg ccgccaccga acggcgcacc cgacggccgc agcaccggaa ccgaattcac
     3061 ggttccggtg gccgagccgg cgggcagagg tgcaagc
//
LOCUS       DATLFO010000079        12556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_130805, whole genome shotgun sequence.
ACCESSION   DATLFO010000079 DATLFO010000000
VERSION     DATLFO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130805"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VM841_02725"
     CDS             <1..826
                     /locus_tag="VM841_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89129.1"
                     /translation="HWDGSAWRRVPGPKVAGPHTFHVVVAGAGSVLLSGSGDNGVDPF
                     LFESDGRSWRRVVLPDLPAPSLQVSDIAVVGSNDLVMLAWNFEIKSSGFGRSDFGVFH
                     RRGGAWTRSLTATDVLDSMVVFPDGSIVVGGYTKTARARDRRTVVKRWNGSRWSDMPA
                     PPEPMTGINGLAGRSATDLWAVGASDPLEVEVSKQPLEATADSKTAEPQRVEVFVSHW
                     NGTKWSSPKAGIDSGYSDINAVDSGPDGTIVAVGWTSGVTGRAPAEASGELILRRC"
     gene            964..2331
                     /locus_tag="VM841_02730"
     CDS             964..2331
                     /locus_tag="VM841_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL89130.1"
                     /translation="MAIDEARLKELGGRFSGSIVVPSDAGYDDAREVFNAMIDKRPAV
                     IAQCASVEDVREALRFGLDAGLDIAVRSGGHSVAGASLNDGGLVVDVRSMNSTEVDPA
                     AKTIRVGGGTNWGEFDAATKPHALAATGGRVTTTGVSGLTLGGGSGWLERKMGLACDN
                     LIGAELVTADGRVVKADETENPELFWALHGGGGNFGIATSLMFRLHDMPTFSGGVLFW
                     KAEAGNEVARAYRDLMAGAPDELGGGFGYITGPPEEFVPEDMQGTIICGVFLFYAGTE
                     AELRSLIGPLLALDPDGSMIMEAPYHDVNGMFDDPPGLQNWWTAEYHDSAPDAMLDEF
                     VALSKTMKPSASQMIMIPWGGAVERVTDSPMANRHSQWVSHPFVLWEDPAENAERIAF
                     GKAFQAMLRTYSNGGTYLNFIGHEGQERVVAAFGEDNYRRLAAVKAEFDPNNVFHGNQ
                     NIKPA"
     gene            2405..3220
                     /locus_tag="VM841_02735"
     CDS             2405..3220
                     /locus_tag="VM841_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVL89131.1"
                     /translation="MSNQAEKAAAFLALHDGPEPLILPNPWDAGSAGLLESLGYRALA
                     TTSGGFAATLGRLDGQVTRDEALAHAATIVAATGLPVSADLENGFGDDAKDVVETVRL
                     AREAGLAGCSIEDFARRDDHPIYDLAHAVERIAAAAEEAHRGDVRMVLTARTENYLHG
                     RRDLDDTIQRLKAFEDAGADVLFAPGVVDPGELRAILEAITRPLNVLIFPGVPKIAEL
                     AEIGVRRISTGSALAYVALGAMVDAARQLRDGSYRGLGPARTGAEAVHTTFGA"
     gene            3356..3946
                     /locus_tag="VM841_02740"
     CDS             3356..3946
                     /locus_tag="VM841_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL89132.1"
                     /translation="MSLRSIPEAVVDEKGADDELAHACIGGDARAWDELVRRYGPYVY
                     AVATRAYRLEPQVAEDVFQDVWIRIYDGLARYRGDGTLRAWIRQVTISACQEHFRRSK
                     KLGVVEPLEEAGAAPAAVTDLDEALDVRLAVNRLSSPCRSTIEMAFFDDLTQAETARR
                     LGAPEGTVAARVSRCLRRLREDLQEKPVQPPSGEMR"
     gene            3943..4329
                     /locus_tag="VM841_02745"
     CDS             3943..4329
                     /locus_tag="VM841_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89133.1"
                     /translation="MNDHLEPEEFAEIAATRTVPPSHVAGCEPCADELKSFLALTSRL
                     RAMPDAPEALSERARIYYPRRRALDDLVASMAMDPALRARLARDPAVVLREAGLEPSP
                     ELIAALTDGERDDSGAGLRLAASLWF"
     gene            4337..6484
                     /locus_tag="VM841_02750"
     CDS             4337..6484
                     /locus_tag="VM841_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVL89134.1"
                     /translation="MKPFTLDDLPRVVRRKRRAPQNAWAEIAVALNPAYARPRLADDV
                     EIADFRYRSGEPYTMMKAPRGPSYIRLTPEDRFIVDLMDGRRTIKDIVVAHFRQYGHF
                     ALSAITSLIEEMRKAGFLSRPYVSVEKAIAVAKRDTGPSWRVRVRRIRAKKRLDMRKP
                     DELTDWLYRHGGRALFGKAANVLYAVVTVVGLVMAWRLLQGNRYSLFGTSAASIFALY
                     GISLLAIFVHEAAHALSCKHFGRKVNAAGFMFYMGMPLFFIETTDTWMANRRQRVLTS
                     WSGPYSGFIIGGACAIAATLMPTNALSAFLYRFAIFSYYGTLQNLVPFLRLDGYYIMM
                     DLIDTQNLRERSFAFLREELFDRIRTRTRLSRHEKVLTAYGVIASVFAVLAILVALAF
                     WSRVFRQPVVNGWNAGPGTRLLILGLGVLVLAAPLRALARLVREAVRRARFVGRLLLR
                     VAQAHWRADAVAVARSLPFLAGLDADALHEIGRRVALRRVPTGRAVITEGEHGSEFFI
                     VRSGRLQVVRTDPDGKEIEVESLGRGSSFGEIALLQSGPRLATVRAVEPSEVFVLDKG
                     TFDRLLADRFDLSGMHGALLSMQELKAMKPFAGLDARDAAEVIRHAEWVSVPPKEAVV
                     RQGREADGFYVIGSGRLEVVKDRKRIGLLGPGDYFGEVALLQDSPRTATVRAVTPSRI
                     LKLDRAGFDAALGSAFSRGSLDSSAQLVREWEH"
     gene            6759..7241
                     /locus_tag="VM841_02755"
     CDS             6759..7241
                     /locus_tag="VM841_02755"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HVL89135.1"
                     /translation="MSKSEVNKVLDRLSRDSRFRARLEKDPKKALAQYTLTAAELKAI
                     SSIPADEAEAFSSTLDTRLAGDRRAASRVAASKVAGSKVAASKLAGSKLAASKVAGSK
                     MAASKVAGSKMAASKVAGSKVAGSKIAASKLAGSKMAASKIAGSKIAGSKIAGSKVSG
                     "
     gene            complement(7296..8528)
                     /locus_tag="VM841_02760"
     CDS             complement(7296..8528)
                     /locus_tag="VM841_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVL89136.1"
                     /translation="MSGILSGMRVVEGSAFVAAPLGGMTLAQMGADVIRFDPIGGGLD
                     YTRWPVTADGRSLFWAGLNKGKRSICIDIKSGEGRDIVQSLVTAPGPDAGMFLTNFPA
                     KGWLSYESLAKARPDLIMIAITGNHDGSSAVDYTVNPATGFPWATGPMNTSFPINHLL
                     PAWDAITGTLAGTGMLAAERYRRITGSGQFAQLALSDVAMAMVGHLGKIAEVQINGAT
                     RPKYGNYLYGAFGRDFATKDGRRLMIVALSKNQWDALRDATGLHEAFTMVEKLMGVNL
                     DDEGGRFEAREVIAAVLKPWVIARSLEEVRTVFDEHKVCWGPYQSFAEMVRDDPRCSE
                     RNPMWREVEQPGIGRYLMPGSPLSFETIPRVPVSPAPRLGEHTDEILADVVGLSGTEI
                     GRLHDAGVVAGVRETAIA"
     gene            complement(8543..9541)
                     /locus_tag="VM841_02765"
     CDS             complement(8543..9541)
                     /locus_tag="VM841_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVL89137.1"
                     /translation="MRIEVAGPGFEDLKVGQILDDAPAITITAGMAALYASLTGDRMR
                     LPLDAALTKAVTGSDDPLAHPGLVWNIAIGASTGLTQRVRANLFYRNMVSLRPVFVGE
                     TLRTRTEVVALKENARRPGKPATGLAVLRMRCENQRGEPVLDFWRCPMLPFRDTEART
                     GYADSFDDIPAEIASEAIHAAIPQWDLDAFRAAVPAERAGDLVAGTTYAVAAREPVTG
                     APGLVRLTLNAAVAHLDPDASAYGRRLVYGGHTISIACSQIVRAVPGIVTVLAWRSCD
                     HTGPVFEDDLLRTDVTVESVEPLPAGGGLADLRAITYATRDDEAPVLDWRLVALVA"
     gene            complement(9650..11869)
                     /locus_tag="VM841_02770"
     CDS             complement(9650..11869)
                     /locus_tag="VM841_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89138.1"
                     /translation="MRIRSLIALSALVAGLATVAEADPPQSPVTQARSVEPVVLTGSQ
                     FPSWSAGPDPAFREPQTPTNYETADTQGSLPATLRSDCYRAGQRWNPYDWSDNGDHNC
                     YQRSRVPANPLEGADVSRLLAYRWNGTDFEQIPFQVDEVFTRYISNNVSGFAFYSGVD
                     EETTYAFDREPFHYNWDAYLDSLPAGNGNPCASRPRPGSPMHNGKATTPDPVRGLDDN
                     DELVFMYSDTGGRAPRKAAMPSGLESTYEVKIVDPSNPLSASYVYVALAAPDGPRPSF
                     DAGNGYVRYVRDANADLFVYSQSSYTGYGAAPKGPYCDLDGTIVTTPRRPQDVPAQPA
                     APNGWAVEQRRPLDTAWVKTPRYWFRYDGRWLMTGLRISPDDSGLSASPNLGPDIVDQ
                     WKARAFQQRPGGRTPCCGYEEEVNNWGGSSILFGERVGAVRAIRAAFGADSSTNNVKT
                     EIFYRDEIRFGDKLRVHVIPPADGIYVQWDYNAGKVSTYYNPWVSTGAPIDGQNDEVF
                     GNTRWHVAQDRIEFRDDDPIPVIGPQHITIPGPGAPSDCRIAGQSGICNDMDFADPFF
                     SGPAATLNWEQISGPNGSLVTRWQVRKHTGGDAYALLAQPYYRDDSCFDDGTGSDPGP
                     HLRSRAVDDGVYGTYVDPQDGLTKSRVCWTPADGDPSDISINDGRPRKFWQGDIATHG
                     VHIQFIADSDNANLTKPVNEIDFEQRMVVLPGNQPNVGERYGRAAEMPLVTVVTPRP"
     gene            12112..>12556
                     /locus_tag="VM841_02775"
     CDS             12112..>12556
                     /locus_tag="VM841_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVL89139.1"
                     /translation="MISQGEIVVRLLIAAAFGALLGLERERKDQPAGLRTFILVAVGS
                     CLFALVSAFGFDDFTGESSDQAVRADVTRISSQIVVGIGFLGGGTILRYGGMVRGLTT
                     AAGLWVTAAVGLAVAMGFWVGASAAVLLTLISLVIKPLEKRLRRAS"
BASE COUNT         1917 a         4160 c         4373 g         2106 t
ORIGIN      
        1 gcattgggac ggatcggcgt ggcggcgcgt ccccggtccg aaggtcgcgg ggcctcatac
       61 gttccacgtc gtcgtcgccg gcgccggttc ggtgctgctc tccggctccg gcgacaacgg
      121 cgtcgacccg ttcctgttcg agtcggacgg acggtcttgg cgccgcgtcg tcttgcccga
      181 cctgccggcc ccgtcgttgc aggtctccga catcgccgtc gtgggcagca acgatttggt
      241 gatgctcgcg tggaacttcg agatcaagtc ttcgggcttc ggacgcagtg acttcggcgt
      301 gtttcatcgg cgtggcgggg cgtggacgcg ttcgttgacc gcaaccgatg tgctcgactc
      361 gatggtggtc ttcccggacg gaagcatcgt cgtcggcggc tacacgaaaa cggcgcgggc
      421 ccgcgatcgc cgaacggtcg tcaagcgctg gaacggatcg agatggagcg acatgcctgc
      481 cccccccgaa ccgatgaccg gcatcaacgg cctcgccggc cggtcggcta ccgacctctg
      541 ggccgtcggg gcgagcgatc cgctggaagt cgaagtatcg aagcagccgc tcgaggcgac
      601 tgccgattcc aaaaccgccg agccccaacg agtcgaggtc ttcgtctcac actggaacgg
      661 aacgaagtgg tcctcaccca aagccggaat cgactccgga tactccgaca tcaatgcggt
      721 ggactccggg ccggacggaa ccatcgtcgc cgtggggtgg acgagcgggg tcaccggacg
      781 agcccctgcc gaagcttccg gcgaactgat ccttcgacgc tgctgacggg ccgatccggt
      841 ccggtcgttc gaggccgccg tccgcggtcg agggacaggc cgtcgcctcc gggcgggagc
      901 catctagacg gatggggccg gaagtcgtac cgtctgaccg aggactcggt ggagggggat
      961 tcaatggcga tcgacgaggc acggctcaag gaactgggag gccgcttttc gggctcgatc
     1021 gtggtgccga gcgatgccgg ctacgacgac gcgcgcgagg tgttcaacgc gatgatcgac
     1081 aagcgtcccg cggtgatcgc ccagtgcgcc tccgtcgaag acgtccgtga agcccttcgc
     1141 ttcggcctcg acgcggggct ggacatcgcg gtgcgttccg ggggtcattc ggtcgccggc
     1201 gcgtcgctca acgacggcgg actggtcgtc gacgtccgct cgatgaacag caccgaggtg
     1261 gatccggccg cgaagacgat tcgtgtcggc ggcggcacga actggggcga gttcgacgcg
     1321 gcgacgaagc cgcacgcctt ggcggcgacg ggcggccggg tcaccacgac cggcgtgtcg
     1381 ggattgacgc tgggcggtgg atcgggctgg ctggagcgaa agatgggact ggcgtgcgac
     1441 aatctgatcg gcgccgagct cgtcacggcc gacggccggg tcgtgaaggc ggacgagacc
     1501 gagaacccgg agctgttctg ggcgttgcac ggcggcggcg gcaatttcgg gatcgcgacg
     1561 tcgctgatgt tccgcctgca cgacatgccc acgttctccg ggggcgttct gttttggaaa
     1621 gccgaagccg gcaacgaggt agcgcgggcg tatcgcgact tgatggccgg cgcacccgac
     1681 gagctcggcg gcggattcgg ctacatcacc ggcccgccgg aagagttcgt tcccgaggac
     1741 atgcaaggca cgatcatctg cggcgtcttc ctgttctacg cgggcaccga agcggaactg
     1801 cgttcgctca tcggcccgct gctggcgctc gaccccgacg ggtcgatgat catggaggcg
     1861 ccctaccacg acgtcaacgg catgttcgac gacccgccgg gactgcagaa ctggtggacg
     1921 gccgagtatc acgacagcgc acccgacgcg atgctcgacg agttcgtcgc gctgtccaag
     1981 acgatgaagc cgagcgcttc gcagatgatc atgatcccgt ggggcggcgc cgtcgagcgc
     2041 gtcaccgact cgccgatggc gaaccggcat tcgcagtggg tcagccatcc gttcgtgctc
     2101 tgggaggacc cggccgagaa cgccgaacgc atcgcgttcg gcaaggcgtt tcaggcgatg
     2161 ctgcgcacgt acagcaatgg cggcacgtat ctgaacttca tcggtcacga aggccaggag
     2221 cgtgtggtcg ccgccttcgg ggaggacaac taccgtcgcc tcgcagcggt caaggcggag
     2281 ttcgacccga acaacgtctt ccacggcaac cagaacatca agcctgcctg agccggaggc
     2341 tcctgaggga gagtcccgcg gaattcgcgc gccgttgaag ccgcgtcgcg ctaaggtcgc
     2401 ttgcatgagc aaccaagcgg agaaagccgc ggcgtttctg gctctgcacg acggtccgga
     2461 gccgctgatc cttccaaacc cgtgggacgc ggggtcggcc gggctgttgg agtcactcgg
     2521 ataccgagcg ctcgcgacca cgagcggtgg gttcgccgcc acgctcgggc gcctcgacgg
     2581 ccaggtgacc cgtgacgaag cgctggcgca cgcggcgacc atcgtggcgg ccaccggcct
     2641 cccggtgtcc gcggacctcg agaacggatt cggcgacgat gcgaaagacg tcgtcgagac
     2701 ggtgcggctg gcccgcgaag ccgggttggc gggctgctcg atcgaggact tcgcacgtcg
     2761 cgacgaccat ccgatctacg acctcgccca cgccgtcgag cggatcgcgg ccgccgcgga
     2821 agaggcccac cgcggcgacg tgcggatggt gctgaccgcg cgcaccgaga actatcttca
     2881 cggccggcgg gatctcgacg acacgatcca gcggctgaag gccttcgagg acgcgggcgc
     2941 ggacgtcctc ttcgcgccgg gcgtggttga tccgggcgag ctccgcgcga tcctggaggc
     3001 gatcacacgg ccgctcaacg tgctcatctt cccgggcgtt cccaagatcg ccgagctcgc
     3061 cgagatcggc gtgcgacgca tctcgacggg cagcgccctg gcctacgtgg cgctcggcgc
     3121 gatggtcgat gccgcgcgac agcttcgcga cggcagctac cgcgggctcg gccccgcgcg
     3181 gaccggcgcg gaggccgttc acacgacgtt cggggcctag ccgcagggtg cttccaaagc
     3241 gtcgcgagga ccgatgcgac gataacgcgg tgtacaagca agcggttgcc ggctcgggct
     3301 catttcccga ttcaaagcct cgcttttcgg gtgccctgcc ggtagcctgt cgccaatgtc
     3361 gctacggtca atcccggagg ccgttgtcga tgagaagggc gccgacgatg agcttgcgca
     3421 cgcctgcata ggcggagacg ctcgcgcatg ggacgagctc gtccgtcgat atggcccgta
     3481 cgtctacgcg gtggccaccc gcgcctaccg cctcgaaccg caagttgccg aggacgtctt
     3541 tcaggatgtc tggatccgga tctacgacgg tctcgcgcgg tatcgcggtg acgggacctt
     3601 gcgcgcgtgg atcagacagg tcacgatcag cgcctgccaa gagcacttcc ggcggtccaa
     3661 gaagctcggc gtcgtcgagc cgctggagga ggctggggcc gcgcctgccg ccgtcaccga
     3721 tctggacgag gcgctcgacg tgcgtctggc cgtgaaccgc ctgagcagtc cgtgccgcag
     3781 cacaatcgag atggccttct tcgacgacct cacgcaggcc gagaccgcgc gccgcctcgg
     3841 cgctcccgaa gggacggtgg ccgctcgcgt cagccggtgt ctgcggcgac ttcgcgagga
     3901 cctgcaagaa aagccggtcc agcccccgtc aggggagatg cgatgaacga ccatctggag
     3961 cccgaggagt tcgcggagat tgccgccacg cggaccgtgc cgccctcgca cgtggccggg
     4021 tgtgagccgt gcgcggacga gctcaagtcc tttctggccc tgacctctcg cctgcgggcc
     4081 atgccggatg ccccggaggc gctgtccgaa cgcgccagga tctactaccc tcgccggcgc
     4141 gcgctcgacg atctcgtggc gtcgatggcg atggaccccg cgctccgcgc gcggctggcc
     4201 cgtgatccgg ccgtcgttct tcgcgaagcc ggcctcgagc cgtccccgga gctgatcgca
     4261 gccctgaccg acggcgagcg cgacgactcc ggcgcgggac tcaggctggc cgcaagcctg
     4321 tggttctgat caccgaatga agccgttcac cctggacgac ctgcctcgcg tcgtccgccg
     4381 gaagcgccgc gcaccccaga acgcctgggc cgagatcgcc gtcgccctca atccggcgta
     4441 cgcgcgcccg cggctcgccg acgatgtgga gatcgcggac tttcgctacc gctccggcga
     4501 gccgtacacg atgatgaagg cgccccgggg gccgtcctac atccgtctga cacccgagga
     4561 ccgcttcatc gtcgacctga tggacggccg gcggactatc aaggacatcg tcgtcgccca
     4621 tttccgccag tacggccact tcgccttgtc ggcgatcacg agcctgatcg aggagatgcg
     4681 aaaggccggc ttcctgagcc ggccgtacgt ctcggtcgag aaggccatcg ccgtcgccaa
     4741 gcgtgacacg ggaccgtcat ggcgcgtgcg tgtgaggcgg atccgggcca agaagcgtct
     4801 ggacatgcgc aagcccgatg agctgacgga ctggctctac cggcatggcg gccgggcgct
     4861 gttcggcaag gccgccaatg tgctctacgc cgtggtcacc gtcgtgggcc tcgtgatggc
     4921 gtggcggctg ctgcaaggga accgctactc gcttttcggc acgtccgcgg cgtccatctt
     4981 cgcgctctac ggcatcagct tgctcgccat cttcgtccac gaggctgcgc acgccctgtc
     5041 gtgcaagcac ttcggccgca aggtgaacgc cgccggattc atgttctaca tgggcatgcc
     5101 gctgttcttc atcgaaacga ccgacacctg gatggcgaac cggcggcagc gggtcctgac
     5161 gagctggtcg gggccctact cgggcttcat catcggcggt gcgtgtgcga tcgcggcgac
     5221 cctgatgccg accaacgcgc tgagcgcttt cctctaccgg ttcgcgatct tctcttacta
     5281 cggaaccctc cagaacctgg tgccgttcct gcggctggac ggctactaca tcatgatgga
     5341 cctgatcgat acccagaacc tgcgcgagcg ctcgttcgcg ttcctgcgcg aggagctgtt
     5401 cgaccggatc cgcacacgca cccgcctcag ccggcacgaa aaggttctca ccgcgtacgg
     5461 cgtgatcgca tcggtgttcg ccgtcctggc gatcctcgtc gcgctcgctt tttggagccg
     5521 cgtcttccgg cagcccgtcg tcaacggctg gaacgcgggc ccgggcacgc gccttctgat
     5581 cctcggtttg ggcgtcctgg tgctggccgc gccgctgcgc gcgctcgcgc ggctggtgcg
     5641 cgaagccgtg cgccgcgccc ggttcgtggg ccggctgctt ttgcgggtcg cgcaggcgca
     5701 ttggcgcgcc gacgccgtgg ccgtggcccg ctcccttccg ttcctggccg gtctcgacgc
     5761 ggacgcgctg cacgagatcg ggcgccgggt cgcgctgcgg cgagtcccca ccggccgcgc
     5821 cgtgatcacc gaaggcgagc acggcagtga gttcttcatc gtccggtccg gacgcctgca
     5881 ggtcgttcgc accgatccgg acggcaagga gatcgaggtc gagtcgcttg gccgcgggtc
     5941 gtccttcggc gagatcgcgt tgctacagtc cggaccgcgg ctggcgacgg ttcgcgcggt
     6001 ggagccctcc gaggtgttcg tgctggacaa aggaaccttc gaccggctgc ttgccgaccg
     6061 tttcgacctg tccggcatgc acggcgctct gctttccatg caggagctca aggcgatgaa
     6121 gccgttcgcc gggctggatg ctcgtgatgc cgcagaggtg attcgccacg ccgaatgggt
     6181 ttcggtcccg ccgaaggagg cggtcgtgcg gcaaggacgc gaagccgacg gcttctacgt
     6241 catcggctcg ggccggctcg aggtcgtgaa ggatcgcaag cgcatcggtt tgctcggccc
     6301 gggcgactac ttcggcgagg tcgccctgtt gcaggactca ccgcggaccg ctaccgttcg
     6361 cgcggtgact ccgtcgcgca tcctcaagct cgaccgagcg ggattcgatg cggctttggg
     6421 gtccgcattc tctcgaggat cattggactc gagcgcgcag ctggtgcgcg aatgggagca
     6481 ctaacccgtg gactgctggc attgtcgaaa gagtgcgctc ggtgtctgcc gcttctgtgg
     6541 ccggggcctg tgcgaggacc acgcgaagac tcacccctac atcctggacc tgttccgatc
     6601 cagcggcggc gactcgatga aggtgatcgt cgtcgaggac gccctccatt gcgggatctg
     6661 caccccccgg cccgacccga tcgaaattcc gagcctgggg tgatgcaagg atccgggcgg
     6721 attctccgtc tagagggtta gacgaaaggg aggtcacaat gtccaagagc gaggtcaaca
     6781 aggtgctgga ccggctgtcg cgcgactcgc ggttccgcgc gcgcctcgag aaggacccca
     6841 agaaggctct ggcgcagtac acgttgaccg ccgccgagct gaaggcgatc tcttccatcc
     6901 ccgccgacga agccgaggca ttctcctcga cgctggacac caggctggcc ggcgaccgcc
     6961 gcgccgcgag ccgggttgcg gcgtccaagg ttgcgggctc gaaggttgcg gcatcgaagc
     7021 tcgcgggctc caagctcgcg gcatcgaagg tcgccggctc gaagatggct gcctccaagg
     7081 tcgccggctc gaagatggct gcctccaagg tcgcgggttc gaaggtggcg ggctccaaga
     7141 tcgcggcgtc caagctggcg ggctcgaaga tggccgcatc gaagatcgcg ggctccaaga
     7201 tcgcgggctc caagatcgcc ggctcgaagg tcagcgggtg acgcgagggg aacacgaatg
     7261 aggcctccgg tggggggcct cattcgcatt ccgggctagg cgatcgccgt ttcgcgcaca
     7321 ccggccacca ccccggcatc gtgcaggcgc ccgatctcgg ttccgctcag cccgacgacg
     7381 tcggccagga tctcgtcggt gtgttcgccc agtcgcggcg caggtgacac cggcacgcgc
     7441 gggatcgtct cgaagctgag cggcgacccg ggcatcagat accggccgat tccgggctgc
     7501 tcgacctctc gccacatcgg attgcgctcg gagcagcggg ggtcgtcgcg caccatctcg
     7561 gcgaacgatt gatacgggcc ccagcagacc ttgtgctcgt cgaagaccgt ccggacctcc
     7621 tccaagctgc gcgcgatcac ccagggcttg agcaccgcgg cgatcacctc acgggcttcg
     7681 aaccggcctc cttcgtcgtc gaggttcacg cccatcagct tctcaaccat cgtgaacgcc
     7741 tcgtgcaggc cggtcgcgtc tcgcagcgca tcccactggt tcttcgacag tgccacgatc
     7801 atcagccgcc gtccgtcctt ggtcgcgaag tcgcgcccga acgcgccgta cagatagttg
     7861 ccgtatttcg gacgggtcgc gccgttgatc tgcacctcgg cgatcttgcc caggtgcccg
     7921 accatcgcca tcgcgacgtc gctgagcgcg agctgcgcga actggccgga ccccgtgatg
     7981 cgccggtacc gctcggccgc gagcattccc gtcccggcca gggtcccggt gatcgcgtcc
     8041 catgccggca gcaggtggtt gatcgggaac gaggtgttca tcgggccggt cgcccacggg
     8101 aagccggtcg cgggattgac ggtgtagtcc accgcgctgg atccgtcgtg gtttccggtg
     8161 atcgcgatca tgatcagatc ggggcgcgcc ttggcgagcg attcgtagga cagccagcct
     8221 tttgcgggga agttggtcaa gaacatgccc gcgtccggac cgggcgcggt gacgagcgac
     8281 tgaacgatat cgcgaccctc gccggacttg atgtcgatgc agatcgagcg cttgcccttg
     8341 ttcagcccgg cccagaacag gctgcggccg tcggccgtga ccggccatcg cgtgtagtcc
     8401 agcccgccgc cgattggatc gaagcgaatg acgtccgcgc ccatctgcgc cagcgtcatc
     8461 ccgccgaggg gcgccgcgac gaaggccgat ccttccacga cgcgcatgcc gctcaagatt
     8521 ccgctcatcg gctctccttc gctcaggcga cgagggcgac gaggcgccaa tccagcacgg
     8581 gcgcctcgtc gtcgcgggtc gcgtaggtga tcgcccggag gtcggcgagg ccgccgccgg
     8641 cgggcagcgg ctcgacagat tcgacggtca cgtcggtgcg cagcaggtcg tcctcgaaga
     8701 cggggcctgt gtggtcgcag gaacgccagg cgaggacggt cacgatcccg gggacggcac
     8761 gcacgatctg cgagcaggcg atcgagatgg tgtggccgcc gtagacgagg cggcggccgt
     8821 aggcgctcgc gtccggatcc agatgcgcga ccgcggcatt gagcgtgaga cgcaccagac
     8881 cgggcgctcc cgtcaccggc tcgcgcgccg cgaccgcgta ggtcgttccg gcgaccaggt
     8941 cgcccgcgcg ctcggccggg accgcagcgc ggaacgcgtc gagatcccac tgcgggatcg
     9001 ccgcgtggat cgcctcggac gcgatctcgg ccgggatgtc gtcgaaggag tccgcgtagc
     9061 cggtcctcgc ttcggtgtcg cggaagggga gcatggggca acgccagaag tcaaggaccg
     9121 gctcgccgcg ctggttctcg catcgcatgc gcaagaccgc aaggcccgtt gcgggcttgc
     9181 cgggtctgcg cgcgttctct ttcagggcga cgacttcggt gcgggtgcgg agcgtctctc
     9241 ccacgaacac gggacgcaac gaaaccatgt tgcgatagaa gagattggct cgcacccgct
     9301 gggtgaggcc cgtggaagcg ccgatcgcga tgttccacac caatcccgga tgtgcgagcg
     9361 gatcatcgct gcccgtgacg gccttcgtca gcgcagcgtc gagcgggagc ctcattcgat
     9421 cgccggtcag ggacgcgtac aacgcggcca tgcccgcggt gatcgtgatc gcaggggcgt
     9481 cgtcgaggat ctggccgacc ttgagatcct cgaacccggg ccccgcgacc tcgattcgca
     9541 tgatgcttcg agcgtaggcc ccggatcggc cggtgccctc cccgtcgcga gtggaagcaa
     9601 gggtgaatcc cctcaccccg tgcgggggag ggagggccgc ttcggttcgc taggggcgcg
     9661 gcgtgaccac ggtgaccagc ggcatctcag cggcgcggcc gtagcgttcg ccgacgttgg
     9721 gctggttgcc gggcaggacc accatgcgct gctcgaagtc gatctcgttg accggcttcg
     9781 tcaggttcgc attgtcgcta tcggcgatga actggatgtg cacgccgtgc gtggcgatgt
     9841 ctccctgcca gaacttgcgc ggccgtccgt cgttgatcga gatgtcggag gggtcgccgt
     9901 ccgcaggcgt ccagcacaca cgcgacttcg tgagcccgtc ctgcggatcc acgtaggtcc
     9961 cgtacacgcc gtcgtccacc gcgcggctgc gcagatgcgg cccgggatcg cttccggttc
    10021 cgtcgtcgaa gcacgagtcg tcacggtagt acggctgcgc gagcagcgcg taggcgtcgc
    10081 cgccggtgtg tttgcgaacc tgccagcgag tgaccagcga gccgttcggg cccgagatct
    10141 gctcccagtt cagcgtggcg gccggacccg agaagaacgg gtcggcgaag tccatgtcgt
    10201 tgcagattcc gctctggccc gcgatgcggc aatcgctcgg cgcccccggc ccggggatcg
    10261 tgatgtgctg cggaccgatg acggggatcg gatcgtcgtc gcggaactcg atgcggtctt
    10321 gcgcgacgtg ccagcgcgtg ttgccgaaca cctcgtcgtt ctggccgtcg atcggtgcgc
    10381 ccgtgctcac ccacgggttg tagtaggtcg ataccttgcc cgcgttgtag tcccactgga
    10441 cgtagatgcc gtcggcgggc gggatcacgt gcacacgcag cttgtcaccg aagcggatct
    10501 cgtcgcgata gaagatctcg gtcttgacgt tgttggtgga ggagtcggcg ccgaacgcgg
    10561 cgcggatggc gcggaccgct cccacgcgct cgccgaacag gatcgacgag ccaccccagt
    10621 tgttgacctc ttcctcgtag ccgcagcacg gcgtgcgccc cccgggacgc tgctggaacg
    10681 ctcgtgcctt ccactgatcc acgatgtcgg gcccgaggtt cggcgaggcc gacagcccgc
    10741 tgtcgtcggg cgagatgcgg agcccggtca tcagccatcg gccgtcgtag cggaaccagt
    10801 accgcggcgt cttgacccaa gcggtgtcga gcgggcggcg ctgctcgacg gcccagccat
    10861 tcggtgccgc cggctgggcc ggcacgtcct gcggccggcg cggcgtggtg acgatcgtgc
    10921 cgtcgaggtc gcagtacggg cccttggggg ccgcgccgta gccggtgtag gacgactgtg
    10981 agtacacgaa caggtccgcg ttcgcgtccc gcacgtagcg cacgtagccg ttccccgcgt
    11041 cgaaggaggg acgcggaccg tcgggcgccg cgagcgcgac gtagacgtag gacgccgata
    11101 gcgggttgct cgggtccacg atcttcactt cgtaggtcga ttccaggccg gagggcatcg
    11161 cggctttccg cggcgccctg ccgcccgtgt cggagtacat gaacaccagc tcgtcgttgt
    11221 cgtcgagccc cctcaccgga tccggcgtgg tggccttgcc gttgtgcatc gggctgccgg
    11281 gacgcggccg cgacgcacac gggttgccgt ttccggcggg cagggaatcc aggtacgcgt
    11341 cccagttgta atggaacggc tcgcgatcga acgcgtaggt ggtctcttca tcgacgccgc
    11401 tgtagaaggc gaagcccgac acgttgttgg agatgtatcg cgtgaagacc tcgtccacct
    11461 ggaaggggat ctgttcgaag tccgttccgt tccatcggta ggcgagcaga cgcgacacgt
    11521 ctgcgccctc cagcgggttg gccggcacgc gcgaccgctg gtagcagttg tgatcgccgt
    11581 tgtcggacca gtcgtagggg ttccagcgct gacccgcgcg atagcagtcg ctgcgcagcg
    11641 tcgcgggaag tgatccttgc gtgtcggcgg tctcgtagtt cgtgggcgtc tggggctcgc
    11701 ggaaggccgg atccgggccc gcggaccagg acgggaactg cgagccggtc agcaccaccg
    11761 gctcgacgct gcgcgcctgc gtgaccgggc tttgcggcgg gtcggcctcg gccacggtgg
    11821 cgaggccggc gacgagcgcc gacagagcga tgagagaccg gatgcgcatg acgaagcctt
    11881 tcgacggctg gactttccgg cagattcgtc gccgcgaccg gcgattcctg cccgggctgc
    11941 ccccgaaggc ggctaggctt taccgaacct gaaagaacgc ttgcctttgg cgggacggtc
    12001 tctcctgcat ccctcgtcgc ccccgacaac gtcgcgcgaa tcatgggaga cgcccgggcc
    12061 ggcttgccgg gatagccgaa aatcgtgacc ctgggccgta aactcgggga cgtgatttcg
    12121 cagggcgaga tcgtcgttcg attgctgatc gcggccgcgt tcggcgcgct gctcgggctc
    12181 gagcgcgaac gcaaggatca gcccgccggt ctgcgcacct tcatcctcgt cgccgtaggc
    12241 tcgtgcctgt tcgcgctcgt gtccgcattc ggcttcgacg acttcacggg cgagtcctcc
    12301 gatcaggcgg tgcgggccga tgtgacccgg atctcgtcgc aaatcgtggt gggcatcggc
    12361 ttcctgggcg gcggaacgat ccttcggtac ggcggcatgg tgcgcggcct cacgaccgcg
    12421 gccggcctgt gggtcaccgc ggcggtcggg ctcgccgtcg cgatgggctt ctgggtgggg
    12481 gcatccgcgg cggtactgct cacgttgatc agcctggtga tcaagccgct cgagaaacgg
    12541 cttcgccgcg cgagcg
//
LOCUS       DATLFO010000080         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_102056, whole genome shotgun sequence.
ACCESSION   DATLFO010000080 DATLFO010000000
VERSION     DATLFO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102056"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..597
                     /locus_tag="VM841_02780"
     CDS             313..597
                     /locus_tag="VM841_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVL89140.1"
                     /translation="MRTEPLASMIESIAFPAPNMPDAELREMIRLGAQTDHHPCGTCA
                     IGEVVDPGLKVLGFDNLWVCDASVMPDNPRANTNFPTMTIAERFVDLLSG"
     gene            948..1472
                     /locus_tag="VM841_02785"
     CDS             948..1472
                     /locus_tag="VM841_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89141.1"
                     /translation="MSKGTRLALAALIGAMALSITSASADKPLVQQQIRQAFKGQLAN
                     PCTTPPDTVAIDGKIHGVAHVHSTNEQNVIRLHLNLADTHGTGTPSGAKYTGNGHINA
                     MVRVAGDLSKGVSFKANFHFHLLRQGQGGDLKIHGQLKGSASTDGVSLDLRLSKIECG
                     PLALSGAASLDVKP"
     gene            1519..2295
                     /locus_tag="VM841_02790"
     CDS             1519..2295
                     /locus_tag="VM841_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89142.1"
                     /translation="MKRIALASALALMVLGVGAAQAADPCSVTLTSPVAGAILRGTVT
                     VGWTFSPGSPPGSYCLPGADATVQLRKSPNGNSFDDLGTVSAAGGSFSWDTTTVKDLA
                     TYSLRIRVPKVNGSSVFSNVVGVTVDNTAPEAIVNSPLPSPADDVCAAAASDTLESCQ
                     TPLLVRASAPDLGYDGSALNGVAYDNLSGVAAVAVTATNDEGTTQLAVTCSSCSAYYT
                     SGTIWSAHIADLPSGLTTFTISTTDRVGNQGEMSATFLLV"
     gene            complement(2414..2578)
                     /locus_tag="VM841_02795"
     CDS             complement(2414..2578)
                     /locus_tag="VM841_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89143.1"
                     /translation="MKLRYRGKDVTVTLYSGITVQPEVEICHTVGIPSKIHVAFDRRV
                     ASVADGAESS"
BASE COUNT          546 a          991 c          958 g          504 t
ORIGIN      
        1 cgtcagaacc gcgagcacga cctcagcctt ctgcttcgcg gtgaagttcg aacgccgtcc
       61 catcgactgc ctccttacca tccgaggcag ctatcgtgtc aactcatcca gctgtcaccg
      121 tggaaccggg gagcgcagcg agcaccgcac tcatgcccgc tggccgcatc tcatggcgct
      181 cggcgaggtc ttgaacagga cgccgactcc cctcctccaa gaccgtcacg tactcggcgg
      241 cgatccgcgc gtagtggaga tcgctgtggc ccttcttgcc tggtcggcgc ggcgagagcc
      301 atttgggacc tgatgcggac cgagccgctc gcgtcgatga tcgaatcgat cgcgttcccg
      361 gcgccgaaca tgccggatgc cgagctgcgc gagatgattc ggctcggcgc gcagaccgac
      421 catcatccgt gcggcacgtg cgcgatcggc gaggtggtcg atcccggctt gaaggtcctc
      481 ggcttcgaca acctgtgggt gtgcgacgcg agcgtgatgc ccgacaatcc gcgcgccaat
      541 acgaactttc cgacgatgac gatcgcggaa cgcttcgtgg atctgctgtc aggatgatgc
      601 cgtcgttgac ggaggcgact cgcgggtcaa acgccgcgtt ataccgcgag agaatttcga
      661 cccggcgaaa gtctccggta aacccggaat agaggggcat tgtgtcccaa agtcgtggcc
      721 ccgccccggg gtcaggttgg gcacgtatct gccagagcac ttatagcact cttaaggcgc
      781 gaaaaggttg taagtagcgc atcggacaag taggatgctc gcttgtactg ccggtctacc
      841 gtcgcggcaa tcaacgcacc ccgtgttgtc ccgactacgc gtagccctcg ggactcgccg
      901 gggggcaagg tcggctcggc taccagggat cccggggggg aaaagcaatg tctaagggca
      961 ctaggttggc gctcgccgcg ctcatcggcg cgatggcgct cagcatcacg tcggcttccg
     1021 ccgacaagcc gctcgttcag cagcagatcc gtcaggcgtt caagggacag ctggcgaacc
     1081 cgtgcacgac tccgccggac accgtcgcca tcgacgggaa gatccacggc gtcgcgcacg
     1141 tgcactcgac caacgagcag aacgtcatcc ggctccacct gaacctcgcg gacacgcacg
     1201 gaaccggaac gccgagcggc gcgaagtaca ccgggaacgg ccacatcaac gcgatggttc
     1261 gtgtcgccgg cgacctttcg aagggtgtct ccttcaaagc caacttccac ttccacctgc
     1321 tccgtcaggg ccagggcggc gacctgaaga tccacggcca gctgaagggc tccgcgagca
     1381 cggacggcgt ctctctggac cttcgccttt cgaagatcga atgcggtccg ctggcgctca
     1441 gcggtgccgc cagcctggac gtcaagccgt agccgagcgc aacggcgaca aacgagaaga
     1501 tcggcgggga tgatcagaat gaaacggatt gctctggcaa gcgctctggc gctcatggta
     1561 ctcggcgtgg gagcggcgca ggccgcggac ccttgctcgg tcacgctcac gtcgcccgtc
     1621 gcaggagcga tcttgcgcgg gaccgtgacg gtcggctgga cgttctcacc cggctcgccg
     1681 cccggctcgt actgtctgcc cggcgccgac gccaccgtgc agctgaggaa gtcgccgaac
     1741 ggcaactcgt tcgacgacct gggcaccgtc tcggccgccg gcggctcgtt ctcgtgggat
     1801 acgacaacgg tcaaggacct ggccacctac tccttgagga ttcgagtccc caaggtgaac
     1861 ggcagctcgg tcttctccaa tgtggtcggg gtcaccgtcg acaacaccgc cccggaggca
     1921 atcgtcaact ctccgcttcc cagccctgcc gacgacgtgt gcgcggccgc ggcttccgac
     1981 acgctggagt cgtgtcagac gccgcttctg gtccgtgcca gcgcgccgga tctgggatac
     2041 gacggttcgg cgctgaacgg cgtcgcctac gacaacctgt cgggtgtggc ggccgtcgcg
     2101 gtgacggcca ccaacgacga gggtacgacg cagctggcgg tgacgtgctc gtcctgcagc
     2161 gcctactaca cctcgggcac gatctggtcg gcccacatcg cggacctgcc gagcggcctg
     2221 accaccttca cgatctccac caccgatcgg gtcggcaatc agggggagat gtcggcgacg
     2281 ttcctcctcg tctgagcaca tcgaagcacg aagagaggcc gccttcgggc ggcttctctt
     2341 gattctcgga ccaagctgcc tcgcatcgcc cgctcgggct cgaacccttc gacaaggccg
     2401 gcgcgatctg cggtcaggac gattccgcgc cgtcggcgac ggacgcgacg cgccggtcga
     2461 acgcgacatg gatcttggag ggaatgccga ccgtgtgaca gatctccact tcgggttgga
     2521 cggtaatccc cgaatagagc gttaccgtga catccttgcc acggtaacgg agcttcacgt
     2581 cgtccacgct ccagcacgaa gtgggtccac caaggaagta gatcgtcgct ccccgacggt
     2641 cgggatcaag gacagcccga gaccagccgc tcggcccgcg aacgaacgcg gccttcttgg
     2701 gcgtgaccga ctgttgcgct ctcgccacgg gtatgtccga cgaaccgcgg aactcgcggg
     2761 cctcgcttcg cgagcaaccg ccgccgagaa ggagcagagc ggccagcgcc cagcccgtta
     2821 gcgtccatgg gcgtggtgta cgacgggacc ctggttctcc ggtaagtgaa gaccaccgtg
     2881 tcatcctcaa gaagtcctga caagactcga gaggagagca cgatggtcgt tcctagcatg
     2941 gaccttggcg actggttgcg caagcagctt gaggaggccg agccggatct gttgcgcga
//
LOCUS       DATLFO010000081         7275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_81247, whole genome shotgun sequence.
ACCESSION   DATLFO010000081 DATLFO010000000
VERSION     DATLFO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81247"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VM841_02800"
     CDS             complement(<1..579)
                     /locus_tag="VM841_02800"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HVL89144.1"
                     /translation="MRKSIPVFTIAFVALMAALPVHSAGSSPLVHASAAQAFGVRVHV
                     ATPVGDPSLTVEPTPFASAADPSVDAVTRTVLGPIRAPEDGTLLGDARALVAEAARTL
                     TPRPASSAEAVVTQVSLFETAGIPLIRADVIHAASSTECTSPSSAKTSAAGSRLVGLS
                     IAGEVIDETPEPNTEIPLVYDNGTPADASDDLS"
     gene            complement(746..2410)
                     /locus_tag="VM841_02805"
     CDS             complement(746..2410)
                     /locus_tag="VM841_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVL89145.1"
                     /translation="MRKVLLPLLALALLATLAPSRAAVVERLRFDNGGVASVEVIYPN
                     SPNQTCESKIKKPWRARYRGALEVVMTDDNRLAIVDEITFPEYLYGLGEVPRSWPEET
                     LRAQVIAARSYALYQLRNPRPGAQSIGYDICSTDRCQVYRGVAVEQGAFGERWVKAVD
                     DTEGMVLTHGGDPVQAFYFSTSSGTTRGNNEVFGGPPRPYFNPVPGKDDGSPMARWTV
                     RIPLAELGPILRRDGLWGSEPITSVRRDGARMVISGGGKTQRPTATSFRSTLNSEASC
                     AFPGKYPPRRSDGSKLPQTVPSIRFEVSVSGSDAVITGRGWGHDVGMSQYGAKALAEE
                     GKSAADILAYYYGGLRPQKITEPGAIRVMVAEGAIKIRITPEGGGTLRTGAGGVIGQG
                     ETYEITPGAGGVKVARVKAGTVEPTLSVEAAGEGTLNEDETFTLPVKLSAPARITAVV
                     LRGETEVRREPESSFERGEHEITVAMTGADGKPLADGTYDVAVEAFDGIDRVRFVTPV
                     AISRPPAPRSSSPIRSLSPLPLIAAAALILLLVGGFFLIRTRSRRT"
     gene            complement(2481..2948)
                     /locus_tag="VM841_02810"
     CDS             complement(2481..2948)
                     /locus_tag="VM841_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL89146.1"
                     /translation="MTREAILVHPADPVVVTAKTMRERHTSSVLVVDDAGALVGILTE
                     RDLVHKIIAEGQSFAALTVGDLMTPNPITADPLDRVWEGARKMSEHRIRHLPVTQQGR
                     PVGVVSHDDIRGMETDPRPTWDVIRGLHASSQEQTLDIEDAPSDLGEIIPAWY"
     gene            3145..4188
                     /locus_tag="VM841_02815"
     CDS             3145..4188
                     /locus_tag="VM841_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HVL89147.1"
                     /translation="MDAFALLAHTVATGASDLHIRVGLPPMVRLHGELLAVGEVPLDD
                     SDTQKMVDQVCSLQQRERLAEEREVDFAIETDDSRFRANVFMERRGYGGVFRVIPTKI
                     KSVEDLGVPQAIIDLCRREKGLVVVTGPTGSGKSTTLAAMIDLINSERNGHILTIEDP
                     IEFVHQPKRCVVTQREVGSHTNSFANALRASLREDPDVILVGEMRDLETISLAITAAE
                     TGHLVFGTLHTMSAPKTIDRIVDAFPADRQEQIRVMLSESLEGVISQALLPTKDGNGR
                     VAAHEILVVLPSIRNIIREAKTHQVQSIMQTGMKHGMVTMDVALERLVQQGLIDADVA
                     RRRLSAGGADDGQ"
     gene            complement(4192..4842)
                     /locus_tag="VM841_02820"
     CDS             complement(4192..4842)
                     /locus_tag="VM841_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89148.1"
                     /translation="MGRILRFRRRGQIPQFEEALARLTEEITLTQSYADAMIRSGQYR
                     AAFAVVDEQRDRLVHAQEQMLAAMRPRHRRGRVAAAGLAAAMLVGSASFAALSSRNDA
                     PSAPSAPAVLERVENRLAEAALVDDERRASTIVAEALADLISIGPELSRDPEIQQQVE
                     SMLRKQIDRLTERRSGTALIAQLNAAVEKVKAEVSEPDPPPPAPEPKPQTDEAPAP"
     gene            5036..5317
                     /locus_tag="VM841_02825"
     CDS             5036..5317
                     /locus_tag="VM841_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89149.1"
                     /translation="MPDAQDKRNKEYDQAAEAATVLDPRLADLWAAIWSSHDEAIPLG
                     MAAALLRLAYLTGYQDALTEADRGSLFTALGLPVPARVKPPGAARRPSR"
     gene            5773..5916
                     /locus_tag="VM841_02830"
     CDS             5773..5916
                     /locus_tag="VM841_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89150.1"
                     /translation="MDRDRVVLPRKIPVIEALACTACGDWWFESAGQRLTAEALVRLG
                     LTG"
     gene            5962..>7275
                     /locus_tag="VM841_02835"
     CDS             5962..>7275
                     /locus_tag="VM841_02835"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HVL89151.1"
                     /translation="MSSPARSRAASSLTSRLRALGAGATLLHVGAHPDDEQSALIARV
                     SLGDGAHAAYWSATRGEGGQNAVGAERGEALGIIRTWESLAARRFDGGEVLYGPFYDF
                     GYSKRGAETLARWGRDRLVDEIVRAIRVVRPQVVVSRWSGGPGDGHGHHQAIGLVIEE
                     AFDAAGEPARGPSLPSWSPVRLYRSLAGDWQAGEEDAAGGDASADGDVWIDAGEIEPE
                     SGRTFEELAREGCNRHRSQGFALLPRPGSARYGYRLLRGPADGGEGFFAGIDTSLAAL
                     CDRAGEPSPAIADALNRAQKAAQRAYESFHPHRPRAVIAALAEGLAALGEAESAARRD
                     GALIREIRRRRAAFAAVVADCSGIRVEASAAAPRATPGSTLPVIVRVWGAAGARVRLE
                     APAGFAVAEGPSTGAEEPETGGPRRFEVRIPVDAAFSVPYWLRESR"
BASE COUNT         1081 a         2474 c         2588 g         1132 t
ORIGIN      
        1 cgacagatca tcggaggcgt cggcgggcgt gccgttgtcg tagacgagcg ggatctcggt
       61 gttcggctcc ggcgtctcgt cgatgacctc gccggcgatc gaaaggccca ccaggcgcga
      121 gccggccgcg ctggtcttgg cgctcgacgg gctcgtgcat tcggtgctgc tggcggcgtg
      181 gatcacgtcc gccctgatca gcgggatgcc ggccgtctcg aacaatgaga cctgcgtcac
      241 gaccgcttcg gcactggatg cgggccgcgg tgtgagcgtg cgcgcggcct cggcgacgag
      301 cgcacgcgca tcgccgagca gcgtgccgtc ttcgggcgcg cgaatcgggc cgagcacggt
      361 tctggtcacc gcgtccaccg acgggtcggc ggcggatgcg aacggggtcg gctcgaccgt
      421 cagcgacggg tcgccgaccg gggtcgcgac gtgaacacgg actccgaagg cctgagcggc
      481 gctggcgtga accagcggcg acgagcccgc cgagtgcacg ggcagcgccg ccatcaaggc
      541 gacgaaggca atagtgaaga caggtatgga cttgcgcatg atctccctcc cctggcgttg
      601 gggttcgccg cgaccggtac gggcttgtcc cgccggatgc ggccgtcgtc attcatcccg
      661 ctaaaacggc cggaggggtg cagcgtttca ttgcaaccga aaggttttca tcggaccagg
      721 cgatccgatc gtcgcaaacc agtagttacg tgcggcgcga acgggtgcgg atcaggaaaa
      781 acccacccac gagcagcagg atcagcgcgg ccgcggcgat cagcgggagc ggagagaggg
      841 accgaatcgg gctcgaggat cggggagcgg gcggacgcga gatcgcgacc ggcgtcacga
      901 agcgcacgcg gtcgatgccg tcgaaggcct cgacggcgac atcgtaggtg ccgtccgcca
      961 gcggcttgcc gtccgcgccg gtcatcgcga cggtgatctc gtgctcgccg cgctcgaagg
     1021 aggattcggg ctcgcggcgt acctcggtct cgccgcgcag cacgaccgcg gtgatgcgcg
     1081 cgggggccga cagcttcacc ggaagcgtga aggtctcgtc ctcgttgagc gtgccttcgc
     1141 cggcggcttc gaccgacagc gttggctcga ccgtgcccgc cttcacgcgc gcgaccttca
     1201 cgccgccggc gcccggtgtg atctcgtagg tttcgccttg gccgatcacg ccgccggcac
     1261 cggtgcgcag cgtgccgccg ccctcgggcg tgatgcggat cttgatcgcg ccttcggcga
     1321 ccatcacgcg gatggcgccc ggttcggtga tcttttgcgg ccgcaggccg ccgtagtagt
     1381 aggcgaggat gtcggcggcg ctcttgcctt cctcggcgag cgccttagcg ccgtactgcg
     1441 acatgccgac gtcgtggccc cagccgcggc cggtgatcac ggcgtcgctg cccgacaccg
     1501 acacctcgaa gcggatcgaa ggcaccgtct gcggcagctt cgatccgtcg cttcgccgcg
     1561 gcgggtactt gcccggaaac gcgcacgatg cctcgctgtt gagcgtcgag cggaacgatg
     1621 tcgcggtcgg acgctgcgtc ttgccgccgc ccgagatgac catgcgcgcg ccgtcgcgac
     1681 gcaccgaggt gatcggctcc gatccccaca gcccgtcgcg ccgcaggatc gggccgagct
     1741 cggcgagcgg gatgcgtacc gtccagcgcg ccatcggcga gccgtcgtcc ttgcccggca
     1801 ccgggttgaa atatggacgc ggcgggccgc cgaagacctc gttgttgccg cgcgtggttc
     1861 ccgaggaggt cgagaagtag aacgcctgca cgggatcgcc gccgtgggtc aggaccatgc
     1921 cttcggtgtc gtcgacggcc tttacccaac gctcgccgaa tgcgccctgc tcgaccgcga
     1981 cgccccgata cacctggcag cggtcggtcg agcagatgtc gtagccgatc gactgcgcac
     2041 cgggacgcgg gtttcgcagc tggtagagcg cgtaggatcg cgctgcgatc acctgcgcgc
     2101 gaagcgtctc ctcgggccac gagcgcggca cctctccgag gccgtacaga tattcgggga
     2161 aggtgatctc gtccacgatc gcgagccggt tgtcgtcggt catcacgacc tcgagggcgc
     2221 cgcggtagcg cgcgcgccac ggcttcttga tcttgctttc gcaggtctga ttggggctgt
     2281 tcggatagat cacctcgacc gaggcgacgc cgccgttgtc gaagcgaagc cgctccacca
     2341 ccgccgcgcg cgacggagcg agcgtggcga gcagtgccaa ggcgagcaac ggaagaagca
     2401 cctttcgcat gggcctccta ccttacccag gaaacgaaag agccgggcca tgcccggctc
     2461 tttcgaaagg aaggggcttg ctagtaccag gcagggatga tctcgccgag gtcgctgggc
     2521 gcgtcctcga tgtcgagggt ctgctcttgc gacgaggcgt gcagcccgcg gatgacgtcc
     2581 caggtcggac gcgggtcggt ttccatgccg cgaatgtcgt cgtgcgagac gacaccgacc
     2641 ggacgtccct gctgcgtgac cggcagatga cggatccggt gctccgacat cttgcgcgcc
     2701 ccttcccaga cccggtcgag cggatcggcc gtgatcgggt tgggcgtcat caggtcgccg
     2761 acggtcaagg ccgcgaagga ctgtccctcg gcgatgatct tgtggacaag gtcccgctcg
     2821 gtcaggatcc cgaccagcgc gccggcgtcg tccacgacga gcaccgacga ggtatggcgc
     2881 tcccgcatgg tcttggcagt cactacgacg gggtccgcgg gatggaccag aatggcctcc
     2941 cgggtcatga cgtgctcaag tcgcatatgg ctctcaccct cctgtcgcgc gtcgtgagcg
     3001 cggctcccgg ccaccttagc gtgggtctcc gaaagtgaat agaaccggcc ggtaacttcc
     3061 cagccggtaa caacgcgtgc ggcccgtgcc cgcttcgccc cgctttcact caagttcggg
     3121 cggggccgtg ccgacccatc gtgcatggac gctttcgcct tgctcgctca taccgtcgcg
     3181 accggcgcat cggaccttca cattcgcgtc gggctgccgc ccatggtgcg cctccacggc
     3241 gagctgctcg ccgtcggcga ggtgccgctg gacgacagcg acacccagaa gatggtggat
     3301 caggtctgct ccctgcagca gcgtgaacgg ctcgccgagg agcgtgaggt cgacttcgcg
     3361 atcgaaaccg acgacagccg cttccgggcc aacgttttca tggaacgacg cggctacggc
     3421 ggcgtcttcc gcgtgatccc gaccaagatc aagtccgtgg aggacctggg tgtgccgcag
     3481 gcgatcatcg acctgtgccg gcgcgagaag ggcttggtgg tggtcaccgg gccgaccggc
     3541 tcgggcaagt ccacaaccct ggcggcgatg atcgacctga tcaacagcga gcgaaacggc
     3601 cacatcctga ccatcgagga cccgatcgag ttcgtgcacc agccgaagcg ctgcgtcgtg
     3661 acccagcgcg aggtcgggtc gcacacgaac tcgttcgcca acgcgctgcg cgcctcgctg
     3721 cgcgaggatc ccgacgtcat cctggtcggc gagatgcgcg acctcgagac gatctcgctg
     3781 gcgatcaccg cagccgagac cggccacctg gtgttcggca cgctccacac gatgagcgcg
     3841 cccaagacca tcgaccggat cgtggacgcc ttccccgccg accggcagga gcagatccgc
     3901 gtgatgctct cggagtcgct ggagggggtc atctcacagg cgctgctgcc gaccaaggac
     3961 ggcaacggcc gcgtggccgc gcacgagatc ctggtcgtgt tgccctcgat ccgcaacatc
     4021 atccgtgaag ccaagaccca tcaggtgcag tcgatcatgc agaccggcat gaagcacggg
     4081 atggtcacga tggatgtcgc gctggagcgc ctcgttcagc agggattgat cgacgcggac
     4141 gtagcgcgac gccggctctc ggcgggcggc gcggacgacg gccagtagct actaaggcgc
     4201 gggtgcttcg tcggtctggg gcttcggctc gggagccggc ggtggcggat cgggctcgct
     4261 cacctcagcc ttgaccttct cgaccgcggc gttgagctgc gcgatcagcg cggtcccgct
     4321 ccggcgttcg gtcagccggt cgatctgctt gcgaagcatc gactcgacct gctgctggat
     4381 ctcggggtcc ctcgacagct cgggaccgat cgagatcagg tccgcgaggg cctcggccac
     4441 gatcgtgctg gcccggcgct cgtcgtcgac gagtgcggct tcggcgagcc ggttctcgac
     4501 tcgctccagg accgcgggag ccgatggcgc ggacggcgcg tcgttgcgcg acgacagggc
     4561 ggcgaacgag gcggagccga cgagcatcgc ggcggcgagg ccggcagcgg cgacgcggcc
     4621 gcgacggtgg cggggacgca tggcggcgag catctgctcc tgcgcgtgga cgaggcggtc
     4681 gcgctgctcg tccaccacgg cgaaggcggc ccggtattgg ccgctgcgga tcatggcgtc
     4741 cgcgtaggac tgggtgaggg tgatctcctc ggtgaggcgc gcaagggcct cctcgaactg
     4801 cgggatctgt cctcggcgac ggaaccgaag gatcctgccc atcccgtcct ccctagaagg
     4861 tcacctcggc tgcggctcaa ccgtaggttt tttcggtgcc tatgtccagc gttctggagc
     4921 attgtccggg tacggcccca ggccgcttgt actggaaggt ttcgacatac cgtcgtttca
     4981 tgcctgcccg ggtttcatgg cgtttctgtc gtacccctgg cctacgcttc cgggtatgcc
     5041 ggacgcccag gataaaagga acaaagagta cgatcaggcc gccgaggcgg cgaccgtgct
     5101 cgatccaagg ctggccgacc tgtgggcggc gatctggtcg tctcacgacg aggccatccc
     5161 cctcggcatg gccgcggcgc tgctgcgcct cgcctatctg acgggctacc aggatgcgct
     5221 gactgaggcc gaccggggaa gcctgttcac ggcgctgggg cttcccgtcc cggcccgggt
     5281 caagcctccg ggcgcagccc gtcggccgtc acgatgagcc gcgcccggca gctcgggcac
     5341 gtcaggtgct cggcttcgcc gtgtgcgaag cgccacgagc acgccggaca ggcgccccag
     5401 gtgacgttcg gatccgatcc gcccaccggc ggcggcggga gcgtgccacc agggacggtc
     5461 agcccgccgg gagggccggc gccgaacggc gacggctgcg tgggcggggg ctgccccgcg
     5521 gccgggccgc ctctcacggc gtttcgggtt ccgcaggcag ggcagatgaa ctcgccggga
     5581 gccgcgatct ccagctccgc gcggcaccgt ccacatcgca cgatcatggc gagcccaatg
     5641 ctatcgagat ttcgaccgca tctgcgggtc aagcagcagg gggcgttgtc cgacactaga
     5701 ggcggagcct gcatgccgaa atcgaacccg atgacccccg tcccagcatg cggcgcgtgc
     5761 ggatccgctt cggtggatcg cgaccgtgtc gtcctgccgc gcaagatccc cgtgatcgaa
     5821 gccctcgcgt gcacggcctg cggcgactgg tggttcgagt cggccggcca gcggctgacc
     5881 gcggaggcgc tcgtgcgtct cggcctcacc ggctagcaga atctaggaag atctccgatt
     5941 gccacataaa ctgtggcaat cgtgtcgagt cccgctcgct cacgcgccgc ttcgagcctg
     6001 acctcgcgtc ttcgcgcgct cggggccggc gccacgctgc tccacgtcgg ggcgcacccc
     6061 gacgacgagc agtcggcgct cattgcccgt gtgtcccttg gcgacggagc ccacgccgcc
     6121 tactggtcgg ccacccgcgg cgagggcgga cagaacgccg tcggggccga gcgaggggaa
     6181 gcgctcggca tcatccgcac ctgggaaagc ctcgcggccc gacgcttcga cggcggcgag
     6241 gtcctctacg gccccttcta cgacttcggc tacagcaagc gtggtgcgga gacgctcgcg
     6301 cgctggggac gggaccggct cgtcgacgag atcgttcgag cgatccgcgt ggtccgcccg
     6361 caggtcgtgg tgtcacgctg gtcgggcggg ccgggcgacg ggcacgggca tcatcaagcg
     6421 atcggcttgg tcatcgagga agccttcgac gccgcggggg aacccgcgcg gggaccgtcg
     6481 ctgccgtcgt ggagccccgt ccgtctgtac cgctcgctcg ccggggactg gcaggccggg
     6541 gaagaagacg ccgccggcgg cgacgcctcc gcggacggcg acgtatggat cgacgccggt
     6601 gagatcgagc ccgagagcgg gcgcaccttc gaggagcttg cgcgggaggg atgcaaccgc
     6661 caccgctccc agggcttcgc gctgctgccg aggcccggaa gcgcacgata cggataccgg
     6721 cttctgcgcg gccccgccga cggaggcgaa ggattcttcg cggggatcga cacctcgctc
     6781 gccgcgcttt gcgaccgggc gggcgagcct tcgccggcga tcgccgacgc gctcaaccgt
     6841 gcgcagaaag ccgcgcagcg cgcctacgaa agcttccatc cccatcggcc gcgtgccgtg
     6901 atcgcggcgc tcgcggaggg actcgccgcg ctcggcgaag ccgagagcgc cgcacggcgg
     6961 gacggtgcgc tcatccggga gatccgccgg cgccgcgccg cgttcgccgc ggtcgtcgcg
     7021 gactgctccg gcatccgggt cgaggcatcg gccgcggcgc cgcgggccac gcccggctcg
     7081 acgctgccgg tgatcgtgcg cgtctggggc gcggccggcg cgcgggtccg tctcgaggcg
     7141 ccggcggggt tcgccgtcgc cgagggcccc tccaccgggg ccgaggagcc ggagacgggc
     7201 gggccgcgtc ggttcgaggt gaggattccc gtcgatgccg cgttttcggt tccctactgg
     7261 ctccgcgagt cccgg
//
LOCUS       DATLFO010000082         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_130843, whole genome shotgun sequence.
ACCESSION   DATLFO010000082 DATLFO010000000
VERSION     DATLFO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130843"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VM841_02840"
     CDS             <1..1719
                     /locus_tag="VM841_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89152.1"
                     /translation="VAQTGRFVSALPDAVREGSASAAAANARGALVLLGIAIASSQVI
                     KPIKGAFQFGLGRRFQALLSRRVMAGVTATPGIAHFEDPAFRDKLEVSEWIDWSPAQS
                     VFVLGQIFQQLVQTISLSVVAARFAGWVPAILLGTAIPAGVAAFNRVKSQGLALWQDT
                     PEIRRASYYRTLALELEAAKEMRLFGLRDWVAGRQDTHWTAGYAETWRKRRFGAWLVM
                     GLHVAMIAGLVVSYWAMLSAALRPAGSPGHIDAGLFLASSLAATAIVTASVGIFTGAA
                     WIRRMNYLLPFVLRLLDLPEKDPRMEARGTRSAHDLPREGVRFEGVHFRYPGTERWIL
                     QGLDLHIQPGQSIALVGENGAGKTTLIKLLCRFYDPTEGRIMLDGIDIREFDLVDLRK
                     RLACIFQDFTRYDLPVRDNIGFGSVEHRFDQAMLSEAARRVGVLEEIGRMPEGWDTPL
                     AREFGGVDLSGGQWQRVALARAMMAQLGQDADLLVLDEPTASLDVRLEHELYEHFAEL
                     SKDRTTLLVSHRFSTVRMAERIVFMEDGRVVEDGTHAELMAHAGRYADLYRLQAEHYL
                     ATGNLE"
     gene            1716..>2892
                     /locus_tag="VM841_02845"
     CDS             1716..>2892
                     /locus_tag="VM841_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89153.1"
                     /translation="MSRRQQLGVGRVLSKIFHTYGAIFGMAWEAARSRAAALFSLMLS
                     LGIIGAFGALVSRSVVDGLAEGDRGKALLFGVIFLSIQGGSTLLNDLMQLLQVDMADR
                     ISHVVDQRMMRIAAGAPGLDHLERSEFADRMKQVRERVWLPSQMLQTMGSAMFVLFGI
                     LASIILLGQIHPILIALPFVMIPSAYIQFRAYRKHWAMFDKAVPEQRLAEHYLKLATS
                     PTGAKEIRLFGLRGHILSEHRRLSDAFVRLMFRARLKQAAGSTFGGILYGLALTGSIA
                     FIGTLALRGQATLGEVAMGVQLTRLMLGHVEMAAELVAGLTEGAFMGEKYLWLLHYEP
                     ALTVAERPLPAPRAIGHGITFENVTFAYPGTEKQVLRDISLHLPAAATVALVGENGAG
                     "
BASE COUNT          409 a          971 c         1020 g          492 t
ORIGIN      
        1 gtggcccaga cgggtcgctt cgtctccgcc ctccccgacg ccgtgcggga aggctctgcg
       61 tcggcggccg cggcgaacgc gcgcggcgcg ctggtcttgc tcggcatcgc gatcgcgagc
      121 agccaggtca tcaagccgat caagggcgcg ttccagttcg ggctcggccg gaggttccag
      181 gcgttgttgt cgcgccgggt catggcagga gtcacggcga cgccggggat cgcgcacttc
      241 gaggaccccg cgttccgcga caagctcgag gtgtcggagt ggatcgactg gtcgccggcc
      301 cagagcgtgt tcgtgctcgg ccagatcttc cagcagctcg tgcagacgat ctcgctgtcg
      361 gtcgtcgccg cacgtttcgc cgggtgggtg ccggcgatcc tgctcggcac cgcgatccct
      421 gccggcgtcg ccgcgttcaa ccgcgtgaag agccaagggc tcgcgctgtg gcaggacacg
      481 cccgagatcc ggcgcgcgtc ctactaccga acgctcgcgc tcgagctgga ggccgcgaag
      541 gagatgcgct tgttcggtct gcgcgactgg gtggccggac ggcaggacac gcactggacc
      601 gccgggtacg cggagacctg gcgcaagcgt cggttcggcg cgtggctggt catgggactg
      661 cacgtcgcca tgatcgcggg gctggtcgtg tcctactggg cgatgctcag cgccgcgctg
      721 cggcctgcgg gctcaccggg gcacatcgac gccggcctct tcctcgcctc gtcgctcgcg
      781 gcgaccgcga tcgtgaccgc gtccgtcgga atcttcaccg gggcggcgtg gatccgccgg
      841 atgaactacc tgttgccgtt cgtgctgcgg ttgctcgacc ttccggagaa ggacccgcgc
      901 atggaggcgc gcggcacgcg cagcgcgcac gacctccctc gtgaaggcgt gcgattcgag
      961 ggtgtgcact tccgctaccc cggcaccgag cgctggatcc ttcaagggct cgacctgcat
     1021 atccagccgg ggcagtcgat cgcgctcgtc ggcgagaacg gcgccggcaa gacgacgctg
     1081 atcaagctgc tgtgccgctt ctacgatccc accgagggcc gcatcatgct cgacgggatc
     1141 gacatccgcg agttcgatct cgtcgatctg cgcaagcggc tcgcgtgcat cttccaggac
     1201 ttcacgcgct acgaccttcc cgtgcgcgac aacatcgggt tcggttcggt cgagcaccgc
     1261 ttcgaccagg ccatgctgtc cgaggctgcg cgccgggtgg gcgtgctgga agagatcggc
     1321 cggatgccgg aggggtggga cacgccgctc gcgcgcgagt tcggcggggt tgatctgtcc
     1381 ggcgggcaat ggcagagagt ggcgctggcg cgcgcgatga tggcgcagct cgggcaagac
     1441 gccgacctgc tcgtcctaga cgagccgacg gcgtcgctgg acgtgcgctt ggagcacgag
     1501 ttgtacgaac acttcgccga gctgtcgaag gaccgcacga ccttgctcgt gtcgcaccgt
     1561 ttctcgaccg tgcgcatggc cgagcgcatc gtgttcatgg aggacggccg cgtcgtcgag
     1621 gacggcacgc acgcggagct gatggcgcac gccggccgct acgcggacct gtaccgactg
     1681 caggccgagc actacctcgc gacggggaac ctcgaatgag ccgccgtcag cagctcgggg
     1741 tcggccgcgt gctgtcgaag atcttccaca cctacggggc gatcttcggc atggcttggg
     1801 aggcggcgcg ctcgcgcgcg gcggcgctgt tctcgttgat gctgtcgctc ggcatcatcg
     1861 gggcgttcgg cgcgctggtc tcgcgcagcg tcgtggacgg gctcgcggag ggcgaccgag
     1921 gcaaggcgct gctgttcggc gtgatcttcc tgtcgatcca aggcgggagc acgctcctga
     1981 acgacctgat gcaactgctg caggtagaca tggcggaccg gatcagccac gtcgtggatc
     2041 agcgcatgat gcgcatcgcg gcaggggccc ccggcctcga ccatctcgag cgttccgagt
     2101 tcgccgaccg catgaagcaa gtgcgtgagc gggtgtggct gccctcgcag atgcttcaga
     2161 cgatgggctc ggcgatgttc gtgctcttcg gcatcctggc ctcgatcatc ctgctcggtc
     2221 agatccatcc gatcctgatc gcgctgccgt tcgtgatgat tccctcggcc tacatccagt
     2281 tccgggcgta ccggaagcac tgggcgatgt tcgacaaggc ggttcccgag cagcgcctgg
     2341 ccgagcacta cctcaagctc gcgacctcgc cgaccggcgc gaaggagatc cgcctgttcg
     2401 ggctgcgcgg ccatatcctc tccgagcacc gacggctgtc cgatgccttc gtgcgcctga
     2461 tgttccgcgc gcgcctcaag caggccgccg gctcgacctt cggcggcatc ctgtacgggc
     2521 tcgcgctcac cggttcgatc gcgttcatcg gcacgctggc gctgcgcggg caggcgacgc
     2581 tcggcgaagt cgcgatggga gtgcagctga cgcgactcat gctcggtcac gtcgagatgg
     2641 ccgccgagct ggtggccggg ctcaccgaag gcgcgttcat gggcgagaag tacctgtggc
     2701 tgctgcacta cgagccggcg ctgaccgtcg cggagcgtcc gctgccggcg ccgcgcgcga
     2761 tcggccacgg gatcacgttc gagaacgtca ccttcgccta ccccggcacc gagaagcagg
     2821 tgctccggga catctcgctg caccttccgg cggccgccac cgtcgccctc gtcggcgaga
     2881 acggcgcggg ga
//
LOCUS       DATLFO010000083         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_71456, whole genome shotgun sequence.
ACCESSION   DATLFO010000083 DATLFO010000000
VERSION     DATLFO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71456"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VM841_02850"
     CDS             <1..277
                     /locus_tag="VM841_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HVL89154.1"
                     /translation="STTVRWRDYKICVPSGRPSLVSTLDGTAGDLDLYVRYNALPTTT
                     SYNCRGISSSPDESCTVTNPSNGWWYVSVYTYNSAGAGTSFQIKATY"
     gene            659..1402
                     /locus_tag="VM841_02855"
     CDS             659..1402
                     /locus_tag="VM841_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89155.1"
                     /translation="MRLWRWWSEVRRRRPILVGAVFWLVFWSVSATAMIWVYSPGGLG
                     DPDRDLRRPSERISRPACVEACDAVTNEDSQPVAAGAGSSGSQRTSGSWAGDDTRISP
                     TPRPGVPRPSLPSIRVPEVSVEVSDVLMIAGSRYLKYVVTVSANDVDGELLITTSVPD
                     KTWFVECTDQAGQQVCTDSFRGADDDHRVVWRQAVGADRKRTLNYLVRLDQFAMPGQE
                     IRNHAHVVWDDKVVSSAPDVFTVPPLIGS"
     gene            complement(1399..1836)
                     /locus_tag="VM841_02860"
     CDS             complement(1399..1836)
                     /locus_tag="VM841_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89156.1"
                     /translation="MLGAEGVHAAPVAVIAGGPMRPPGLFVQENMHPVFTFCSFPSFE
                     TATSTTIATFVIRPNTHEAHFQVTNRCGTYDETFRLGECRQFRPSQWDCDHRRPDGFR
                     AWATYAILDGKPHIDAGEYKESDPSNVYWWVNGFLLAPAITSI"
     gene            2087..>2629
                     /locus_tag="VM841_02865"
     CDS             2087..>2629
                     /locus_tag="VM841_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89157.1"
                     /translation="MRSGLMSCRRRLSLRRAVVGLLAIALIAVFAPAAGADTTSQIAV
                     QEKRLKWLEGVARQKQARVASIQASMRSLTAQIRESNAELDRLDRRWKRHQDQLIDTM
                     AAYDRLRGQMDTASIEMYMRGPTHYVGAVMGAETLSDVGDAVNYTGALLSRNRRLAEQ
                     TALATHEITALREQDEAMIAQ"
BASE COUNT          446 a          789 c          912 g          482 t
ORIGIN      
        1 gtctacgacc gtgcgttgga gggattacaa gatctgcgtg ccgagcggcc gtccgagcct
       61 ggtctccaca ctcgacggaa ccgccggtga cctggatctg tacgttcgct acaacgccct
      121 gccgacgacg acttcgtaca actgccgtgg catctcgtcc tcgccggacg agagctgcac
      181 ggtgacgaac ccgtcgaacg gctggtggta cgtcagcgtc tacacgtaca actcggccgg
      241 tgcaggaacg tcgttccaga tcaaggctac gtactagaca ccactcgctc agcgaagcgg
      301 cctcctcttg gggccgcttc gctttccatg cccatcgcgg ccgccgtcat gcctgcggtg
      361 tcgctccggg tcgcgcgccg accggtccgt cccggatgtg gctccgaacg gactcgtcaa
      421 gaaagttgac gtgtcgccgc tctgaggcgg cggcggtccg gtccgtcccg gatgttccga
      481 acggacgtca agaaagttga cgagtcgccc ggcccgacgg ttccggcatt cagtccgccg
      541 gacgaatgtc gtcccatgtg gcccgtccgg gctatagcag gaccggcatg gcggggcatg
      601 gggtcggggt ggggccgcat ccgatatcgg aagcatgagc gggcttgtcg gagggcctat
      661 gcggctgtgg cgctggtgga gtgaggtgcg ccgccgccgt ccgatcctgg ttggggccgt
      721 gttttggctg gtgttttgga gtgtgtcggc cacggcgatg atctgggtct attcgccggg
      781 cggtcttggg gatcctgatc gggatctacg caggccgtcg gagcggatca gccggcccgc
      841 gtgcgtggaa gcctgcgatg cggtgacaaa cgaggattcg cagcccgtcg cggccggtgc
      901 cgggtcgagc ggctcgcagc ggacgtccgg atcatgggcc ggggatgaca cgaggatctc
      961 gccgacgcct cgtccgggag tgccgcgacc ctcgcttccg tcgatccgtg tcccggaggt
     1021 gtcggtcgag gtctccgatg tgctgatgat cgcggggagc cgctatctga agtacgtggt
     1081 gaccgtgtcc gccaacgacg tcgacggcga actcctgatc accaccagcg tccccgacaa
     1141 gacgtggttc gtggaatgca cggatcaagc cggtcagcag gtttgcaccg acagttttcg
     1201 tggtgccgac gacgaccatc gggttgtttg gcgacaagcg gtaggtgccg accggaagcg
     1261 cacgcttaat tatctggttc gcctcgatca gttcgcgatg ccgggtcagg agatcaggaa
     1321 ccatgcgcac gtggtatggg atgacaaggt cgtcagttct gccccggacg tgtttacggt
     1381 gccgccgtta atcggctcct agatacttgt gatggccggc gcgagcagga aaccgttgac
     1441 ccaccagtag acgttcgacg ggtcggattc cttgtactcg cccgcgtcaa tgtgaggctt
     1501 gccgtccaga atggcgtagg tcgcccacgc acggaaccca tcgggccgac ggtggtcaca
     1561 atcccattgg ctcggacgga actggcggca ctcgccaagc ctgaaggtct cgtcgtaggt
     1621 accgcatcgg ttagttacct gaaaatgcgc ctcgtgggtg ttggggcgga tgacgaacgt
     1681 tgcgatcgtg gtcgaggtcg cggtttcgaa gctgggaaac gagcagaagg tgaagaccgg
     1741 atgcatgttc tcctggacga agagacccgg ggggcgcatg gggcctccgg cgatgacggc
     1801 gactggtgcg gcgtggacgc cttcggctcc gagcatggcg gcggttaagg cggccgcggc
     1861 gatgatgatt ctcccccgca ttcgattccc ccctggattc gaaccttcat ggtacggccg
     1921 gccggccggg ccggtaaaga gggatttcgg ccgatcagtc gtattcgctc gagccgagaa
     1981 cccagatgaa ggactggatg tcgatcatgt cccggggcgc ggcgaagcag gacggcgccg
     2041 cagaattaca ttcaagtaat actggcggcc ggacgagatg tcggatatgc gtagcgggtt
     2101 gatgtcgtgt cgccggaggt tgtcgctgcg ccgggcggtc gtgggtctgc tcgcgatcgc
     2161 gctgatcgcc gtcttcgctc ccgcggccgg agccgacacc acgtcgcaga tcgcggtgca
     2221 agagaagcgg ctgaagtggc tcgaaggggt cgcgcgtcag aagcaggcgc gcgtcgcgtc
     2281 catccaggcc tcgatgcgtt cccttaccgc gcagatccgc gagagcaacg ccgagctcga
     2341 ccggctcgac cggcgatgga aacgacatca ggatcagctc atcgacacga tggccgccta
     2401 cgaccggctg cgcggacaga tggatacggc ttcgatcgag atgtacatgc gtggtcccac
     2461 gcattacgtc ggcgcggtca tgggcgccga gacgctttcg gacgtcggcg atgccgtcaa
     2521 ctacacgggc gcgctgctgt cgcgaaaccg ccgcctcgcc gagcagaccg cgctcgccac
     2581 gcacgagatc accgcgcttc gcgagcagga cgaggcgatg atcgcgcag
//
LOCUS       DATLFO010000084         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_1754, whole genome shotgun sequence.
ACCESSION   DATLFO010000084 DATLFO010000000
VERSION     DATLFO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VM841_02870"
     CDS             <1..1031
                     /locus_tag="VM841_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89158.1"
                     /translation="IVLADDHGRVALWSPSMERMTGVSAAEAAGKQVGFLLRGRGLHG
                     EAVSVDVAGEADTFDLELIFPDGQVRWLHGRHGRGQAADGSMAFDVIVVNDVTRRREV
                     DRLKSDFISTVSHELRTPLTPIKGFAALLLRRGEELPAARRREALSTILDHTEHLGRL
                     VEDLLLASTMSGEGERRMPALRTQPVSLAAVLEKTQRTFRASRPHRELIVSCEDGARA
                     IGDSLRVEQILANLVSNAFKFSDDGTPVTVEARSVNGEAIIEVHDLGRGIPADKTEEI
                     FERFKRLEDPLVMETGGAGLGLYIARKLARGMSGDVRVESEPGRGSTFRVHLPRAASE
                     AGRASVAG"
     gene            1180..2277
                     /locus_tag="VM841_02875"
     CDS             1180..2277
                     /locus_tag="VM841_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVL89159.1"
                     /translation="MQRGVRIKRAVARAGLVCLLVSATLVYVQLEKSVTVRIEGEIRP
                     VKTFALTVGDALERAGIDVAEGDRVTPPPGTRVREGTLIKIVRAKPVTIILNGTPREV
                     VVTASTVDEVIRQVALRSSMRDYVGASRSARVYPGMTIEYRQAVAVTVVHDDKSERVI
                     TNAPTVRQMLSELGISLGAKDRVQPGLDAYPTHGMTVKVVRVGERKETVEKVLAAPTQ
                     TKRSTDVEFGVTKVIQEGRAGLRVMRYLSTYVDGKRVKRTLLESKVVRKPQPRIVAIG
                     AGFPSCPCNKGTQSGVASWYDAYGLTAAHKTLPKGTVVKVTNLENGKSVNVVIRDRGP
                     YVKGRIIDLSDNAFSRIASLGTGVVRVKIAY"
     gene            2286..>2730
                     /locus_tag="VM841_02880"
     CDS             2286..>2730
                     /locus_tag="VM841_02880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HVL89160.1"
                     /translation="MATPLGRRETKEILARHGLKPKTALGQHFLVDPNTIMRVLDLAG
                     VQPGEQVLEVGPGLGTLTIALQSAGARVVAVEHDRGLQPALEEVLLGRDRVQVVWGDA
                     MTVDLRKLLRGRPTKLVANLPYNISVPLLMQVLEDIPEIAECTVMV"
BASE COUNT          427 a          944 c          956 g          403 t
ORIGIN      
        1 gcatcgtgct ggccgacgat catggccgcg tggcgctgtg gagcccgtcg atggagcgca
       61 tgaccggcgt gagcgcggcc gaggccgccg gcaagcaggt cgggtttctg ctgcgcggcc
      121 gcgggctgca cggcgaggcc gtctcggtgg acgtggccgg cgaggccgac accttcgacc
      181 tcgagctgat ctttcccgac gggcaggtgc gctggttgca cggccgtcac ggccgcggcc
      241 aggccgccga cggctcgatg gccttcgacg tcatcgtcgt caacgacgtc acccgccgcc
      301 gcgaggtcga ccggctcaag agcgacttca tctcgaccgt ctcgcacgag cttcgcacgc
      361 cgctcactcc gatcaagggt ttcgccgcgt tgctgcttcg ccgcggcgag gaactgccgg
      421 ccgcccgccg ccgcgaagcg ctttccacga tcctggacca caccgagcat ctgggccggc
      481 tggtggaaga cctgctgctg gcctcgacga tgtcgggcga aggggagcgg cgcatgcccg
      541 cgctgcgaac ccagccggtg tcgctggcgg cggtgctcga gaagacgcaa cgcaccttcc
      601 gcgcctcacg gccgcaccgc gagctgatcg tgtcgtgcga agacggcgca cgcgcgatcg
      661 gcgactcgct gcgcgtggag cagatcctgg ccaacctggt gtcgaacgcg ttcaagttct
      721 ccgacgacgg tacgccggtg acggtcgagg cccgaagcgt caacggcgag gccatcatcg
      781 aggtccacga cctcggccgc ggcatcccgg cggacaagac cgaagagatt ttcgagcggt
      841 tcaagcgcct cgaggatccg ctcgtcatgg agaccggcgg cgccgggctc ggcctataca
      901 tcgcccgcaa gctcgcgcgc ggcatgagcg gggacgtgcg ggtcgagtcc gagcccgggc
      961 gcggcagcac gttccgggtc cacctgcccc gggcggcttc cgaggccggc cgggcgagcg
     1021 tcgccggcta agccccggcc gccgcttccc cgtgtccggt ttgcccggtt tgaagcccgg
     1081 gcacccgctt ctgttagcct tgcgcccgtt gcctggggcc ggaggcccgc cggcgccgcg
     1141 agatccgcgg ggcgggcgcg catccggaaa gcgacacgta tgcagcgggg cgtccggatc
     1201 aaacgagccg tcgcgcgagc gggccttgtg tgcctgctcg tgtcggccac gctcgtctac
     1261 gttcagctcg aaaagagtgt cacggtccgg atcgaaggcg agatccggcc ggtcaagacc
     1321 ttcgcgctca cggtcggcga cgcgctggag cgggccggga tcgacgtcgc cgaaggcgat
     1381 cgggtcacgc cgccgcccgg cacccgcgtc cgcgagggca cgttgatcaa gatcgtgcgc
     1441 gccaagcccg tcacgatcat cctcaacggc acgcctcgcg aggtggtcgt gaccgcatcg
     1501 acggtggacg aggtcatccg ccaggtcgcg ctgcgatcgt cgatgcgcga ctacgtgggc
     1561 gcctcgcggt cggcgcgcgt gtacccgggc atgaccatcg aatatcgcca ggccgtcgcc
     1621 gtgacggtcg tgcacgacga caagagcgag cgggtcatca ccaacgctcc gaccgtccgg
     1681 cagatgctgt ccgagctcgg catctcgctc ggtgcgaaag accgtgtgca gccgggcttg
     1741 gatgcctatc ccacgcacgg gatgacggtg aaggtcgtgc gcgtcggtga gcgcaaggag
     1801 accgtcgaga aggtcctcgc cgccccgacc cagaccaagc gctccaccga cgtcgagttc
     1861 ggcgtgacga aggtgatcca ggaaggccgc gccgggctca gggtcatgcg ctacctgtcc
     1921 acctacgtgg acggcaagcg ggtcaagcgc acgctgctcg agtccaaggt cgtgcgaaag
     1981 cctcagccgc gcatcgttgc gatcggcgcc ggcttcccgt cgtgcccgtg caacaagggc
     2041 acgcagtccg gcgtggcgtc ctggtacgac gcctacggcc tcacggccgc gcacaagacg
     2101 ctgcccaaag ggacggtcgt gaaggtgacc aacctcgaga acggcaagag cgtcaacgtc
     2161 gtcatccgcg accgcgggcc ctacgtcaag ggccggatca tcgacctgtc ggacaacgcc
     2221 ttcagccgca tcgcgtcgct cggcacgggc gtcgttcgcg taaagatcgc ctactgagcc
     2281 cctccgtggc cacgccgctc ggtcgccgcg aaaccaaaga gattctcgcc cgtcacggcc
     2341 tcaagcccaa gacggcgctc ggacagcatt tcctcgtcga tcccaacacg atcatgcgcg
     2401 tgctcgacct ggccggcgtg cagccgggcg agcaggtgct cgaggtcggg ccgggactgg
     2461 gcacgctgac catcgcgctg caaagcgccg gcgcgcgggt cgtcgccgtc gagcacgacc
     2521 gcgggcttca gccggcgctg gaggaggtgc tgctggggcg cgaccgcgtt caggttgtgt
     2581 ggggcgacgc gatgacggtc gatctgcgaa agctgctgcg cggccggccg accaaactgg
     2641 tcgcgaacct gccctacaac atctcggtgc cgctgctgat gcaggtgctc gaggacattc
     2701 ccgagatcgc cgagtgcacc gtgatggtgc
//
LOCUS       DATLFO010000085         5154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_102411, whole genome shotgun sequence.
ACCESSION   DATLFO010000085 DATLFO010000000
VERSION     DATLFO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102411"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2506
                     /gene="carB"
                     /locus_tag="VM841_02885"
                     /pseudo
     CDS             98..2506
                     /gene="carB"
                     /locus_tag="VM841_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(4123..4839)
                     /locus_tag="VM841_02890"
     CDS             complement(4123..4839)
                     /locus_tag="VM841_02890"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HVL89161.1"
                     /translation="MRSLRTAVCLAVAAASLSLPGSVRAAVSSPSPGATVRGAVAISE
                     NEGGTKPFYCGSDGLDSTITVRRVSDSAVVHQASKGSGGGWTTTWTTHGLPIGAYRIV
                     SVAASKNGTCSVVESTLSDFQVTLENFSSVTYTGETNAGIAFPFTASARLVDQFTGLA
                     IQGRSVTFSIGETPVGNCVTGADGRCSTRASAVASPGSHTIKVSFAGDCCWRSSSSTA
                     TLTVTPLPPCIVDPVSCVPA"
     gene            4946..>5154
                     /locus_tag="VM841_02895"
     CDS             4946..>5154
                     /locus_tag="VM841_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89162.1"
                     /translation="MTRHRRSSRPLRWIAAASLLCIGVAVAAPDANPGWIRVFGGEGV
                     DVALGLAGDSDGGVVVSGGFQRELR"
BASE COUNT          861 a         1713 c         1682 g          898 t
ORIGIN      
        1 gatggtcggc tcgaaggacg cgagcgtctc tttggtgatg tcgttgggga tctcgtcgag
       61 ccggtagccg acggcgagct tggcggccat cttggcgatc ggcgtggaca ccggcggatc
      121 caacatccag ttcgcgatca acccgcagga cggccgcatg gtcgtgatcg agatgaaccc
      181 gcgcgtgtcg cgctcgagcg cgctcgcctc gaaggccacc ggcttcccga tcgccaagat
      241 ggccgccaag ctcgccgtcg gctaccggct cgacgagatc cccaacgaca tcaccaaaga
      301 gacgctcgcg tccttcgagc cgaccatcga ctacgtcgtg gcgaagatcc cgcgcttcac
      361 cttcgagaag ttccccggaa tcgagccggt gctcggcacc agcatgaagt cggtcggcga
      421 ggtcatggcg atcggccgga cgttcccgga ggcgctgcag aagggcatcc gctcgatgga
      481 gcagaaccgt gccggcctca acggagacgg cttcgacccg cccgaggacc tcgtcacgat
      541 tctggagcgg ctgacgcgac cgcgtccgga ccggctcttc gacgtcgaag cggcgctacg
      601 cgcgggcgcg agcgtcgagc aggtccagga cgtcaccggc atcgacccgt ggttcctgac
      661 gcagatccgc cgcatcgtgc ggatgcgcga gaccctggag cgcgacggcc gcaacgacgg
      721 gcggatgcgc gacgcgaaac ggctcggctt ctccgacaag cagatcgcca cgctcaccgg
      781 cgggaccgaa tcggacgtgc gcgccgagcg agaagccgcg ggcatcgtcc cggtgttcaa
      841 ggcggtggac acctgcgccg ccgagttcga ggccgagacg ccctacttct attcgaccta
      901 cgaggacgag tccgaggtcc ggccgggcac gcgtcccgcc gtcgtgatcc tggggtcggg
      961 ccccaaccgg atcgggcaag gcatcgagtt cgactactgc tgcgtgcacg ccgcgttcgc
     1021 gctcgacgac gccggctacg acacggtcat ggtcaactgc aaccccgaga cggtctcgac
     1081 cgactacgac accagcgacc ggctctactt cgaaccgctc acgctcgagg acgtgctcgc
     1141 cgtcgtgcgc gccgagaacc cggtcggcgt catcgtcacg ctcggcgggc agacgccgct
     1201 caagctcgcc cgcgggctcg ccgaggcggg cgtgccgatc ctgggcacgc agcccgaggc
     1261 gatcgacctg gccgaggacc gcggccgctt cggcgaggtg ctcgaccaac tcggcctcga
     1321 cgcgcccccg tggggccttg ccaactcggt tgccgaggcc cgcgagatcg cgcaacgcat
     1381 cggcttccct gtgctcatcc gtccgtccta cgtgctcggc gggcgcggca tgatgatcgc
     1441 ctacgacgac gagcgcctcg acgcctacgt gcggagcgcc accgagatct cgcccgatca
     1501 ccccgtgttc cttgaccggt tcctcgagga cgcgatcgag gtcgacgccg acgcgatctt
     1561 cgacggcgat gagctctacc tcggcggtgt gctcgagcac atcgaggagg ccggcatcca
     1621 ctccggcgac tcggcctgcg tgctgccgcc gcatacgctc tcgcgcgcgc agatcggcca
     1681 gatccgcgac gcgaccgaaa agctcgcccg cgcgctcgga gtgcgcggct cgctcaacgt
     1741 gcagttcgcg gtccaagacg gccgcctcta cgtgatcgag gccaacccgc gcgccagccg
     1801 caccgtgccg ttcgtgtcga aagccaccgg cgtgccgatg gcgaaggtcg cggcccgcgt
     1861 catggcgggg gagtcgctcg cgcagttgcg cactgccggc ctcatccccg aaagggaccc
     1921 gatgatcgac ctgcgcttcg ggcacgtcgc cgtgaaggaa gccgtgctgc cgttcagccg
     1981 cttccccggt gtggacacgc tgctcggccc cgagatgaag tccaccggtg aggtcatggg
     2041 gatcgaccgt agcttcggcg tcgccttcgc caaggccgag gaagccgcgt tcggtgcgct
     2101 gcctcgaagc ggcagcgtgt tcatctcggt taccgatcgc gacaagcggg cgatggtcct
     2161 gcccgcgcgc cagctccacg cgatgggctt cgagctagtc gcgaccggcg gcaccgccga
     2221 cctgctgcgc cgcaacggcc tgccggtcaa ggaggtcaac aagatcggtg aagaaggcga
     2281 gaatgtcgcc gacctgatcg cgcgcggtga gatcgcgctg atcattaaca cgcccaccgg
     2341 ctcatccggg ccgcgcgccg acggcgacta catccgcact gcagccgtcc gtcacagcgt
     2401 gccggccatc accaccctcg ccggcgtgct cgccgccgtc caaggcatcg aagagctcaa
     2461 gggccacgag gccggcgtga tgagcctgca ggagtacctg gcgtgagccg cgatcggagg
     2521 ctcatgaagc gatgtaccgc ctcacgatta ttgcttcgaa ctatggttgg cagtccggag
     2581 ccgttctcgc gcctgagcga ggtgagcctt gacggttcct tcccgaatgc gaagcgtctc
     2641 ggcgatttcg gcttctgcca tgtcttcgag gtatcgcaga acaacgcatg ctctctgccg
     2701 cgggggcagc ctctcaagag atttgagcaa tgcgatattt gcggtcgatt cgtcgagcca
     2761 ggagcctcgc tccgattccc tgtatctcaa gaaagctctc atctcgacgc gccttcttcg
     2821 aagccggttc atgttcaagc gtgtaatcgt ggtgcggagc catgcacgtc gctgggcgat
     2881 ggactggtcc gggggagctc tctcgactgc acgcacaagc gcgtcttgca ctaggtccga
     2941 tgcctcctga tcatctctgc tgagcagccg ggcgacggtg agggcccagg gataaagttc
     3001 gtggagctga tccgagaggg gtcgaacggt gtcagtgctc tgggtccagt cttgattggg
     3061 catgttcatc agctgatcgt tacgcgaacg ggttctgacc acacaacatg tcggctgcct
     3121 tcagagaaga tcgtccacgc gcctaggacc tcgtagtcgc cttcgagtac gcgcccattt
     3181 gccgatcgct gtccttcgca tccgaccccc gtccagtcga ccggattctc cgtggtgtag
     3241 gtctgcccgg gacgtagggg caccgagact tggttgtcgg ggaaaactcc tccggtggcc
     3301 gcgaaatagc tatgccaatt ccagacggca gtgccatctc ttcgcgccca atactcgacc
     3361 gagcccccgt taaagagaaa cctgacggtc gccgatcctt cgttcgtgag cttcatcgtt
     3421 agtcggattg accggcgatc gtccaggacg ttccgggagg cttcgagaga tgcgaccagc
     3481 ggagtcgagc cgtttgtcga tgcaggaggc gaggcacact caaatggagg cgcgtcatta
     3541 cgtgatcctg gcgaggtcgg tgtcctcggc gtctggatgt atcgtggaac aagaatgacg
     3601 gcgagggtta caatgattcc gagggtgccc acccgaaaga cctgtcgaat aaaggcgcgc
     3661 cgcgacacgc ggtttcgcac ctgcctctcg acctgctcga acgctgcttt ctcagctctt
     3721 gattgaattg cgtcaccaag caattcaagc tggaccgcga ctggttcctt gctcaccatg
     3781 accccatctc tccttcgaag gcgtctcatg attagagaca ctccgcaggc ccttccaggg
     3841 ttagcgatgg cacagttggg actgcccaca gttcggttga ctcgtagctt gtgagccttg
     3901 tcatgctgct gccacctgcg agtagacggc ctcaaactct accggcgtga gccgtccgag
     3961 gcgtcgatgg cgccggcgac tacatccgca ccgcagccgt ccgccacgat gtgcccgcaa
     4021 tcaccaccct cgcaggcgtg ctcgccgccg tccaaggcat cgaagagctc aagcaccacg
     4081 agcccggcgt gatgagcttg caggagtacc tggcgtgacg gcctaggccg gcacgcagga
     4141 gaccgggtcc acgatgcacg gcggcagggg agtgaccgtg agcgtggccg tcgacgagct
     4201 cgatcgccag cagcagtcgc ctgcgaacga gactttgatc gtgtgcgacc cggggctcgc
     4261 cacggcgctg gcgcgcgtgg agcatctccc gtccgccccc gtcacgcagt tgcccacggg
     4321 ggtttcaccg atggagaacg tcacggatcg gccctggatg gccagtcccg tgaactgatc
     4381 gacgaggcgt gccgacgccg tgaaagggaa tgcgatccct gcgttggttt cgccggtgta
     4441 ggtcacgctg ctgaagtttt cgagcgtcac ctggaagtcc gacagcgtcg attcgacgac
     4501 ggagcatgtt ccgttcttcg atgccgcgac cgacacgatc cggtaggccc cgatcggcaa
     4561 tccgtgcgtc gtccaggtcg tcgtccatcc gccgccggat cccttgctcg cctgatgcac
     4621 gacggccgag tcggacacgc ggcggacggt gatcgtggag tccaagccgt cggagccgca
     4681 gtagaacggc ttcgtccctc cttcgttttc gctgatcgcg accgccccgc gaaccgtggc
     4741 gccgggcgac ggagagctga cggccgcccg gacgctgccg ggaagcgaga gcgatgcagc
     4801 cgcgactgcg aggcagacgg cggtacgaag ggatcgcatg gtttcctcct gagacggccg
     4861 gttaccttgc tggagttcct cgccgccgca gcgatctcct gcttgtcgaa tcgcctcgcg
     4921 gcgcgattgt agaatccggt cgcgcgtgac tcgtcatcgg cgttcttccc gtccgctccg
     4981 ctggatcgcg gccgcatcgc ttctgtgcat cggcgtcgcc gtggcggcgc cggatgcaaa
     5041 cccggggtgg atccgcgtct tcggcggcga gggcgtggat gtcgccctcg gcctggccgg
     5101 tgactcggac ggaggagtcg tcgtctcggg cgggttccag cgtgagctga ggat
//
LOCUS       DATLFO010000086        10756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_11524, whole genome shotgun sequence.
ACCESSION   DATLFO010000086 DATLFO010000000
VERSION     DATLFO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11524"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VM841_02900"
     CDS             <1..776
                     /locus_tag="VM841_02900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVL89163.1"
                     /translation="ASGSSRGSGRGYYRLHYHPGNLIFAAAGNVRHEDVVEAVGKAYA
                     HAPAGPTRIARTGDTAPPLRNEVHVAERKLEQSHVVYGTQGLARNDPDRFALGVLNIV
                     LGGGMSSRLFQEIREKRGLVYSVWSSHTGYAETGLFTVYAGTSPKRVREVLSLVRDEI
                     AAVVEQGITPEELARGKGHLTGSLVLGLEDTGSRMSRLGKGELCHNEILSPDEVVERI
                     KGVTEDDVIAVGRRVLTEQPWALTVLGQTDGNGFDEFVR"
     gene            943..1737
                     /gene="dapB"
                     /locus_tag="VM841_02905"
     CDS             943..1737
                     /gene="dapB"
                     /locus_tag="VM841_02905"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HVL89164.1"
                     /translation="MIRVGVIGAAGKMGRQVCQAVHADPGLILAAAIDSTHAGTPVGR
                     LTGDPSITVEISDELDRLSDAEVEVAVDFTHPKVVKDNVRWCIRHAIHAVVGTTGLSA
                     ADLDDIRGLIDEEGNESNVFVAPNFAVGAVLMMAFARRAAKHLPNVEIIELHHDGKAD
                     APSGTALRTAEGIVAERGEPRPLAMGETHPGARGAAVGGIRVHSVRLPGLVAHQEVIL
                     GGPGETLSIRHDSYDRESFMPGVVLAVKAVPSKPGLTLGLESLLDL"
     gene            1734..3359
                     /locus_tag="VM841_02910"
     CDS             1734..3359
                     /locus_tag="VM841_02910"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HVL89165.1"
                     /translation="MTHPYVVEDFTPDERATLDRVVGNTDSNVFVLMNLPEAVKGALF
                     ARYSRTAKSLRRLFLDEFLRDGDIESLGASAGSDLGMAKADELYGRIFIEFGDDSVAQ
                     LGGAHIAVEQASNILTKQLEWGRLAAYLEQSTRYVGYDDMPGGRWRYYLEPDIMASPH
                     AAAYERTLDAIFERYAAALRRAIEHFAQRYPKHEGDPDRVWKATIKAKACDVLRGMLP
                     AATVSNLGIYASGQAYEMMLMRMMASPLREARDTAAVMLTELNKVIPTFLQRVDQPDR
                     GGTWIEYLRSTRQSMEQIAAEILGEDKPEERPLVTLTDFDPDAEVKLVAAMLYAFSSL
                     PEDTLIDKARAMSTDERLRVMRTYVGERANRRHKPNRAFERVDYRFDVLGDYGGFRDL
                     QRHRMLTIEWQDLTPLHGAELPPEADECGAGDVYRASLDDSGALWESIAPDLRPQAQY
                     AVCMAYRIRYAMQMNLREAMHLIELRSSAQGHAGYRRVAHEMHRLIGEQAGHRAVAEF
                     MSYVDYSSVDLERLEAERRAERKRLESLERDEP"
     gene            complement(3397..3534)
                     /locus_tag="VM841_02915"
     CDS             complement(3397..3534)
                     /locus_tag="VM841_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89166.1"
                     /translation="MITWVLVQEPTAAVGAAIEMIEERVGSTAEVMWLCPDELAEPVQ
                     D"
     gene            complement(3888..9827)
                     /locus_tag="VM841_02920"
     CDS             complement(3888..9827)
                     /locus_tag="VM841_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89167.1"
                     /translation="MTLLAALFSQTVARSAPEAPVVAADASEITERVIIFDGVNPVFM
                     HALKSVTDYELVHEYEIIRGVAVSVPAASVESVISFAQRNGLRAERDMEIHADLSNSA
                     RTVYVKANGVNSGAWGLGYTGAGVTVAIIDSGLYAQHQSLDDQDDNSATNDPKIVLFK
                     DYVNAGGAEVAPYDDNDHGTHVAGIASGTGGGGANIGMAYRSNLAGFKVFNAAGAGSS
                     SSFIAAVNWIVQNGHTVDPPIRVINYSGGSTPVGGNNNGNSAQSLAVDAAMDAGFLVA
                     VAGGNGSQDGAGPNQVQGNVNIPADSRKGVAVCSSNSANPPAAPTYSNWDSEGPTGDG
                     RIKPDVCAPGDGVNSANSPANGGLPCPAPLSCGGPNSYANFGGTSMSSPHVAGIAALI
                     FQAAPNITANQMRQVLFETALLYPTKDNNRGYGGVNARAAIELAIARHGGSSPLSVTA
                     NGPYTAIQGEPVTVTATATGGVPPHSYAWDLDNNGSFETAGKSVSFPHTATSGAKIVK
                     VRVTDSAGTPSTSTATGTVNVLSRVDVFSDSVGATDNGWTSNDATLGWHRTEVRAKSP
                     TWSWYPGNDVLQLYMSNTDTTMTRTVDLSALTGSVRLRFARSGDIEPDFDYLRVKAKR
                     TADASYTTLAEYSDMLGSTWQTETVDLSAFAGSQINLQFQFFSDEFIEETGPFLDDFE
                     VFGTGSPVVDTTPPGAIGDLSPASVTESSVTLSWTATGDDGAVGRASFYDIRYSTTPI
                     SAANFDAATPVPNAPEPLPSGSAESFAVTGLVPNTQHYFAVKAIDDGANYGPISNVPS
                     ATTLADLAAPSQVTGLTGTPGNRSATVSWAAATDNVAVAGYEVFKDGVLAASPTGTAA
                     TITGLTNGVSYEFKVRAKDTAGNVGAFSDPVSVIPEAPAPVTGWLMAGGDAARTGFTP
                     GAGSIAVPNILQTLTAPDAILSSPLIVDLDRDGRSDVVVVQDATSSSSAIVVRAYRQT
                     ATGLVPMWSSPVPQNPALGQPATGGFARIAAGDLDGNGTPEIVVNANYETRVQGTSLN
                     DGTVRLLRGSDGALLGQPVATTIGLPVSPAIGDLDGNPDTLEVAYTHQVGGTSSGYYV
                     SVLSYNGTALNLLMDLNVGANALLASPALADLRPTAGNEIVVAESAGTQRVLICSAAT
                     GTCPAANTLGVGQSVRGLSVADLNNDGVPEIVANATQAESLKVIRTSPTLSAVGRADG
                     YLWNTASLRDVDGDGFTEIVNVNYSEFVNDFDRIGNVAVRGYNGSAVTVKGNLTRTPP
                     PGESSQSRGGASLADIAGDGRPEMIFGAGNGRLVAVQFNASGVPSELWSVALGSPAIN
                     APVAVGDVTGDGRLDLVTAGSDGRITVIGAGPLASIRLSPATATVSAGQSQSYTVEGF
                     DSVGNSLGDVTAATTLSITGGTCSGASCSSTQAGDRVVTATYQGKTANATLTVTPGPL
                     ASMTIAPSSSAITAGASQAYMVTGADAYGNSLGDVTPGTGFSITPNGSCSANTCTATV
                     SGPHTVTATRSSVTVQASLQVNPGPLASLALSPAATTVVLGNGQAYTAQGRDAFGNSL
                     GDLTAGTTFAIFPDGSCNANVCTPARLGDHTVRAARGMAQGSAIMHVIPDTIHRIVIS
                     PANATITAGDSQAFTAEGFDGDGNSVGDVTGATVFTIGPDGSCSANVCTATVAGNKTV
                     LGAVAQTSAAAGLKVVAGPMTGISLTPAVSQIPSGGTRVYTVLGRDQYGNATGDVTSS
                     ATLAIAPDGSCSAASCTATAYGDHTVTATVTPHTATATLKVVPQAPSITTPAAGSVNG
                     SSIEVTGGAPAGTTITLWDNGFKIAENVAVAPDGSWAVTRSFAEGNHSMTAFAVAGSN
                     VSAPSPARLFSVDATAPTTTIYRRQGYVVAQVYHPLERVVVEGVARDTTSGVAEVEVS
                     YAGTSTVVDRVACSGPCTEFRWTSTPNLAPGVYTVRAVPIDRAGNRGSAATITIASTS
                     LISGGGGSASSPASIRPNVVE"
     gene            10215..>10756
                     /locus_tag="VM841_02925"
     CDS             10215..>10756
                     /locus_tag="VM841_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVL89168.1"
                     /translation="MATPFAKDGSLDLDGAVKLASHLADHGSDGVVLAGTTGESPTLT
                     REEKRELFSAVVPAVRGRASVIAGTGTYSTAESVELSRMAAGCGVDALLVVTPYYSRP
                     PQAGLEAHFRTVAAATDLPVMLYDIPARTARKIEHETFLRLADVPGIAGVKDAAGDAR
                     DVCQAQEGLVLDLAGGARRDV"
BASE COUNT         1613 a         3619 c         3817 g         1707 t
ORIGIN      
        1 gtgcaagcgg tagtagccgt ggatctggtc gcggctacta ccgcttgcac taccacccgg
       61 ggaacctaat cttcgccgcg gccggcaacg tgcggcacga ggacgtggtc gaggcggtcg
      121 gcaaggctta cgcgcacgcg ccggcggggc cgacgcgcat cgctcggacc ggcgacaccg
      181 ctccgccgct gcgcaacgag gtgcacgtcg ccgagcgaaa gctggagcag tcgcacgtcg
      241 tctacggaac gcagggcctg gctcgcaacg atccggaccg gttcgcgctg ggcgtgctca
      301 acatcgttct gggcggcggc atgtcgtcgc gcctgttcca agagatccgc gagaagcgcg
      361 gcctggtcta ttcggtctgg tcctcgcaca ccggctacgc cgagacgggg ctgttcacgg
      421 tctacgccgg gacctcgccg aagcgagtgc gcgaggtgct gtcgctggtc cgtgacgaga
      481 tcgccgcggt cgtcgagcag ggcatcacgc ccgaagagct tgcgcgcggc aaggggcatc
      541 tgaccgggtc gctggtgctg ggcctggagg acacgggcag ccggatgagc cggctcggca
      601 agggcgagct gtgccacaac gagatcctgt cgcccgacga ggtcgtggag cgcatcaagg
      661 gtgtgaccga ggacgacgtg atcgcggtcg gccgccgcgt cctgaccgag cagccgtggg
      721 ccctgacggt tctcggccag accgacggca acggcttcga tgagttcgtc cgctgaccga
      781 tccgcgacga gccgggccgg tgacggcctc ggccgcgtcg agttcgtcca ctgattcgtt
      841 cctggtcacg ttgaggtcct atacgtgccc gaacgcgacc agaagccgat gcgacgcggc
      901 ggttcggttg cggtcgtcgg gtccgggtag catggcgcga tcatgattcg ggtgggagtc
      961 atcggcgcgg ccggcaagat gggccggcag gtctgtcagg ccgtgcatgc cgatcccggg
     1021 ctgatcctgg ccgccgcgat cgactcgact cacgccggca cgcccgtcgg gcgcctcacc
     1081 ggggacccgt ccatcacggt cgagatcagc gacgagctgg accgcctgtc cgacgccgag
     1141 gtcgaggtgg cagtggactt cacccacccg aaggtcgtca aggacaacgt gcgctggtgc
     1201 atccggcacg cgatccacgc ggtcgtgggc acgaccggct tgtcggcggc cgacctcgac
     1261 gacattcgcg gcctgatcga cgaggaaggc aacgagtcca acgtcttcgt ggcgccgaac
     1321 ttcgccgtcg gcgccgtgct gatgatggca ttcgcgcggc gcgccgccaa gcatcttccc
     1381 aacgtcgaga tcatcgagct tcaccacgac ggcaaagccg acgcgcctag cggcaccgcc
     1441 ctgcgcaccg ccgaagggat cgttgccgaa cgcggcgagc cgcggccgct cgcgatgggc
     1501 gagacccatc ccggcgcgcg cggcgccgcc gtcggcggca tccgcgtgca cagcgtccgc
     1561 ctgcccggac tggtcgcgca tcaagaggtg atcttgggcg gcccgggcga gacgctctcg
     1621 atccgccacg actcctacga ccgcgagtcg ttcatgcccg gagtcgtgct cgcggtcaaa
     1681 gccgttccgt cgaagccggg cctgacgctc gggctggaaa gcctgctgga cctatgaccc
     1741 atccctacgt cgtcgaagac ttcacgcccg acgagcgcgc gaccttggat cgcgtcgtgg
     1801 gcaacaccga ctcgaacgtc ttcgtgctga tgaacctgcc cgaggccgtc aaaggcgcgc
     1861 tgttcgcccg ctactcgcgc accgccaagt cgttgcggcg gctctttctg gacgagttcc
     1921 tgcgggacgg cgacatcgag tcgctgggcg cgtcggccgg atccgatctg ggcatggcca
     1981 aagccgacga gctctacggc cgcatcttca tcgagttcgg cgacgactcg gtcgcgcagc
     2041 tgggcggcgc gcacatcgcg gtcgagcaag ccagcaacat cttgaccaag cagctcgagt
     2101 ggggacggct ggcggcgtat ctggagcagt ccacgcgcta cgtcggctac gacgacatgc
     2161 cgggcgggcg ctggcggtac tacttggagc ccgacatcat ggcctcgccg cacgcggcgg
     2221 cctacgaacg gaccttggac gccatcttcg agcggtacgc ggccgcgctg cggcgcgcga
     2281 tcgagcactt cgcgcagcgc tatcccaagc acgaaggcga ccccgatcgc gtctggaaag
     2341 ccacgatcaa agcgaaagcc tgcgacgtgc tgcgcggcat gctgcccgcc gcgaccgtca
     2401 gcaatctggg catctacgcc agcgggcagg cctacgagat gatgctcatg cgcatgatgg
     2461 cctcgccgct gcgcgaagcg cgcgacaccg cggccgtcat gctgaccgag ctgaacaagg
     2521 tcattcccac gttcctgcag cgcgtcgatc agccggaccg tggcggcacg tggatcgagt
     2581 atctgcgcag cacgcgtcaa tcgatggagc agatcgccgc cgagatcctg ggcgaggaca
     2641 agccggaaga acggccgctg gtcacgctca ccgacttcga tcccgacgcc gaggtcaagc
     2701 tggtcgccgc gatgctctac gcattctcat ccttgccgga agacacgctg atcgacaagg
     2761 cgcgcgcgat gagcaccgac gagcgtctgc gggtgatgcg cacgtacgtg ggggagcggg
     2821 cgaaccggcg gcacaagccc aaccgcgcat tcgagcgcgt ggactaccgc ttcgacgtgc
     2881 tgggtgacta cggcggcttc cgcgatctgc agcgccaccg catgctgacg atcgaatggc
     2941 aggacctgac gccgctccac ggtgcggagc tgccgcccga ggccgacgag tgcggcgccg
     3001 gcgacgtcta ccgcgcctcg ctcgacgaca gcggcgcgtt gtgggagtcg atcgcgccgg
     3061 atctgcggcc gcaggcgcag tatgcggtgt gtatggccta ccggatccgt tacgcgatgc
     3121 agatgaactt gcgcgaggcg atgcatctga tcgagctgcg ctcgagcgcg caaggacatg
     3181 ccggataccg gcgggttgcg cacgagatgc accgtttgat cggcgagcag gccggccacc
     3241 gggcggtagc ggagttcatg tcctacgtgg actactcgtc ggtggatctt gaaaggcttg
     3301 aggcggagcg gagggcggag cggaaacgtc tcgaatcttt ggagcgggac gagccctagg
     3361 cgatgggggc gccgggcgcg ccgatggttc gagcaactag tcctggacgg gctcggcgag
     3421 ctcgtcggga cagagccaca tcacttcggc ggtcgacccg acgcgctcct cgatcatctc
     3481 gatcgccgcg ccgacggccg ccgtcggttc ctgcaccaac acccaagtaa tcatcctcac
     3541 tcctctcctc ggcgcaagga ttcaacgccg tcgaagccga tccttccggg gtcccgccac
     3601 aatttccgag ccaccgccgg gatttacgac ccaaagcgga acaatgccct tctaccggtc
     3661 gcgcgtccca ccgctgcatc ttcaaaacga gccttccgac cagcggaaac ggccccctga
     3721 agatcatccg ggttggtgcc gtcggacggg atcgggacgg ttcgatcgca tgcgcccggg
     3781 cgcttcggac accggatcgg gacaagcagg gcggctcgcc ggcagggccg cgcaaacgag
     3841 gccgggaggg ccgcatcggc cctcccggca gcttcgtttc ggttgcgcta ttccacgacg
     3901 ttcggccgga tcgaggcggg cgacgaggcg ctgccgccgc ccccgctgat caggctcgtg
     3961 gacgcgatcg tgatggtcgc ggccgagccg cggtttccgg cccggtcgat cggaacggcg
     4021 cgcaccgtgt agacacccgg cgccaggttc ggcgtcgagg tccagcggaa ctcggtgcac
     4081 ggacccgagc aggccacgcg gtccacgacc gtcgaggtgc cggcgtagct cacctcgacc
     4141 tcggccacac ccgaggtcgt atcgcgggcg acgccctcga ccaccacacg ctcgagcggg
     4201 tggtagacct gcgccacgac gtagccctga cggcggtaga tcgtcgtcgt gggagcggtc
     4261 gcgtccacgc tgaacagtcg agccggcgac ggcgcgctca cgttggatcc cgcgaccgcg
     4321 aacgcggtca tcgagtggtt gccctcggcg aacgagcgcg tcaccgccca ggatccgtcg
     4381 ggtgcgaccg cgacgttctc ggcgatcttg aacccgttgt cccagagcgt gatcgtcgtg
     4441 ccggccggcg cgccgccggt gacctcgatc gagctgccgt tcacgctgcc ggccgccggc
     4501 gtcgtgatcg acggcgcctg cggcacgacc ttgagcgtcg cggtggccgt gtgcggcgtg
     4561 accgtggcgg tgaccgtgtg gtcgccgtag gccgtggccg tgcaagaagc ggcgctgcac
     4621 gagccgtccg gcgcgatcgc gagcgtcgcc gacgacgtca cgtcgccggt cgcgttgccg
     4681 tactggtcgc gtccgagaac cgtgtagacg cgggtgccgc ccgacgggat ctgcgacacc
     4741 gcgggggtga gcgagattcc ggtcatcgga ccggcgacga ccttgaggcc agcggccgcc
     4801 gatgtctggg cgaccgcgcc caggaccgtc ttgttgcccg cgacggtggc cgtgcagacg
     4861 ttggcgctgc acgatccgtc cggcccgatc gtgaagacgg tcgcgccggt gacgtcgcct
     4921 accgagttgc cgtcaccgtc gaagccctcg gccgtgaagg cctggctgtc gccggcggtg
     4981 atggtggcgt tcgcgggcga gatcacgatg cggtgaatcg tgtcggggat cacgtgcatg
     5041 atcgccgatc cttgggccat gccgcgggcc gcgcgaacgg tgtggtcgcc caggcgtgcc
     5101 ggggtgcaca cgttggcgtt gcacgagccg tcggggaaga tcgcgaaggt cgtgcccgcc
     5161 gtcaggtccc cgagcgaatt accgaaggcg tcccggcctt gtgccgtgta cgcctggccg
     5221 ttgccgagca cgaccgtcgt cgcggcaggg ctgagtgcca gcgacgcgag cggacccggg
     5281 ttcacctgca gcgaagcctg gaccgtgacc gagctgcgcg tcgcggtgac ggtgtgcggg
     5341 ccggagacgg tggcagtgca ggtgttggcc gagcacgagc cgttcggcgt gatcgagaac
     5401 ccggttcccg gcgtgacgtc cccgagcgag tttccgtacg cgtcggcgcc cgtgaccatg
     5461 taggcctggc ttgcgccggc ggtgatcgcc gacgaggacg gagcgatcgt catcgacgcg
     5521 agcggaccgg gcgtgaccgt gagcgtcgca ttggcggtct tgccctggta ggtcgcggtg
     5581 acgacgcggt cgccggcctg cgtgctcgag cacgacgccc ccgagcaggt tccgcccgtg
     5641 atcgacaacg tcgtcgcggc cgttacgtca ccgagcgagt tgcccaccga gtcgaagccc
     5701 tcgaccgtgt agctctggct ctgtcccgcg ctcaccgtcg cggtggcggg ggagagccgg
     5761 atcgaggcaa gcgggccggc gccgatgact gtgatgcgcc cgtcggatcc ggccgtgacc
     5821 agatcgaggc ggccgtcgcc ggtgacgtcg ccgacggcga ccggcgcgtt gatcgccggc
     5881 gagccgagcg cgaccgacca cagctcggac ggcacgccgg aggcgttgaa ctgcacggcc
     5941 accagacgcc cgttcccggc gccgaagatc atctccggac gcccatcccc ggcgatgtcg
     6001 gccagggacg cgcccccgcg cgactggctc gactcgcccg gcggcggcgt gcgggtcagg
     6061 ttgcccttca ccgtgaccgc cgagccgttg tagccgcgga ccgccacgtt gccgatcctg
     6121 tcgaagtcgt tgacgaactc cgagtagttg acgttgacga tctcggtgaa tccgtcgccg
     6181 tccacgtcac gcagcgaggc ggtgttccac agatagccgt cggctcgtcc cacggccgac
     6241 agcgtcggcg aggtgcggat gaccttgagg ctctcggcct gcgtggcgtt ggccacgatc
     6301 tcgggaacgc cgtcgttgtt cagatccgcg accgacagcc cgcgcacgga ctgccccacg
     6361 ccgagcgtgt tcgccgccgg gcacgtcccc gtggccgccg agcagatcag gacccgctgc
     6421 gtgcccgcgc tttcggcgac gacgatctcg ttgccggccg tgggacgcag gtccgccagc
     6481 gccggcgatg cgagcagtgc gttcgccccg acgttgaggt ccatcagaag gttcagcgcc
     6541 gtcccgttat aggaaagcac cgacacgtag tacccggagc tcgtgccgcc gacctgatgc
     6601 gtgtaggcga cctccagcgt gtccgggttc ccgtcgaggt ccccgatcgc cggcgagacc
     6661 ggcaggccga tggtcgtggc gaccggctgg ccgagcagcg cgccgtcgga gccgcggagc
     6721 aggcggacgg ttccgtcgtt cagcgacgtg ccctgaacgc gcgtctcgta gttggcgttc
     6781 accacgatct cgggcgtgcc gttgccgtcc aagtcgccgg cggcgattcg cgcgaagccg
     6841 ccggtggccg gctgcccgag cgccgggttc tgcggcaccg gggaggacca catcgggacc
     6901 agccccgttg cggtctgacg gtatgcccgc acgacgatcg cgctcgacga ggacgtcgcg
     6961 tcctggacca cgaccacgtc gctgcggccg tcacggtcga gatccacgat gagcggcgac
     7021 gacaggatcg cgtccggtgc cgtcagcgtt tgcaggatgt tcgggaccgc gatcgagccg
     7081 gcgcccggcg tgaagcccgt ccgcgcggcg tcgccgcccg ccatcagcca gcccgtcacc
     7141 ggcgccggcg cctccgggat gacgctcacc ggatcgctga aggcgcctac attcccggcc
     7201 gtatccttgg cacggacttt gaactcgtag ctcacgccgt tggtcaggcc ggtgatggtc
     7261 gcggccgtcc cggtggggga tgccgcgaga acgccgtcct tgaacacctc gtaaccggcg
     7321 accgcgacgt tgtcggtggc cgcggcccac gacacggtgg cggagcggtt gccgggcgtg
     7381 ccggtcaggc cggtcacctg cgagggcgcg gccagatctg ccagcgtggt cgccgacggc
     7441 acgttcgaga tcggcccgta gttggcgccg tcgtcgatgg ctttcacggc gaagtagtgc
     7501 tgcgtgttcg ggaccagacc cgtcacggcg aaggactccg ccgagcccga cggaagcggc
     7561 tcgggcgcgt tcggcaccgg agtggcggcg tcgaagttgg ccgcgctgat cggggtggtg
     7621 ctgtagcgaa tgtcgtagaa cgaggcgcgc ccgaccgcgc cgtcgtcacc ggtggccgtc
     7681 cagctcagcg tcaccgacga ttccgtcacc gacgcgggcg acaggtcgcc gatggcgccc
     7741 ggcggtgtgg tgtccaccac cggcgatccg gtgccgaaga cctcgaagtc gtccaggaac
     7801 ggtccggtct cttcgatgaa ctcgtccgag aagaactgga actgcaggtt gatctgcgag
     7861 ccggcgaatg ccgacaggtc caccgtctcg gtttgccacg tggagccgag catgtccgag
     7921 tactcggcca gcgtggtata gctggcgtcg gccgttcgct tggccttcac gcgcaagtag
     7981 tcgaagtccg gctcgatgtc cccgctgcgt gcgaaccgca atcgaaccga tccggtcagc
     8041 gcggacaggt ccaccgtgcg tgtcatagtg gtgtcggtgt tggacatgta gagctggagc
     8101 acgtcgttgc ccgggtacca cgaccaggtc gggctcttcg cccgcacctc cgtgcgatgc
     8161 cagccgagcg tggcgtcgtt cgacgtccag ccgttgtccg tcgccccgac gctgtcgctg
     8221 aacacgtcga cgcgcgagag cacgttgacc gtgcccgtcg ccgtcgaggt ggacggcgtt
     8281 cccgccgagt cggtcacgcg caccttgacg atcttggcgc ccgacgtcgc ggtgtgcggg
     8341 aacgagaccg acttgccggc cgtctcgaaa gagccgttgt tgtcgaggtc ccaggcgtag
     8401 ctgtgcggcg ggacgccgcc ggtggcggta gccgtgacgg tcaccggttc gccctggatc
     8461 gcggtgtacg ggccgttcgc ggtgaccgac aacggggagg agccgccgtg tcgggcgatg
     8521 gccagctcga tcgccgcgcg cgcgttcacg ccgccgtagc cgcgattgtt gtccttggtg
     8581 ggatacagca atgcggtctc gaacagcact tgccgcatct ggttcgcggt gatgttcggc
     8641 gcggcctgga agatcagggc ggcgatgccg gccacgtgcg gcgaggacat cgacgtgccg
     8701 ccgaagttgg cgtacgagtt cgggccgccg cacgagagcg gagccgggca gggaagcccg
     8761 ccgttcgccg ggctgttggc cgagttcacg ccgtcgccgg gagcgcacac gtcgggcttg
     8821 atgcggccgt cgcccgtggg gccctcggag tcccagttcg aatacgtcgg tgcggcgggc
     8881 gggttggcgc tgttgctcga gcagacggcg acgcccttgc gcgagtcggc cggaatgttg
     8941 acgttgccct gcacctggtt ggggccggcg ccgtcttgtg agccgtttcc accggccacg
     9001 gcgaccaaga agcccgcgtc catcgcggcg tctacggcaa ggctttgcgc gctgtttccg
     9061 ttgttgttgc cgccgaccgg ggtcgagccg ccggagtagt tgatcacgcg gatcggcggg
     9121 tccacggtgt gtccgttctg aacgatccag ttgaccgccg cgatgaagct cgacgacgag
     9181 ccagccccgg cggcgttgaa gaccttgaag cccgcgaggt tcgaccggta cgccatgccg
     9241 atgttcgcgc ccccgccacc ggtaccggac gcgatgccgg ccacgtgcgt gccgtggtcg
     9301 ttgtcgtcgt agggcgcgac ctccgcgccg ccggcgttca cgtagtcctt gaacagcacg
     9361 atcttcgggt cgttcgtcgc gctgttgtcg tcctggtcgt cgaggctttg gtgctgcgcg
     9421 tacagaccgg agtcgatgat cgcgacggtc acgcccgcgc cggtgtaccc gaggccccac
     9481 gcacccgagt tcacaccgtt cgccttgacg tagacggttc gcgccgagtt cgacaggtcg
     9541 gcgtggatct ccatgtcgcg ctcggcccgc agcccgttcc gctgcgcgaa ggagatcacc
     9601 gactcgacgc tcgcggcggg caccgaaacc gcgacgccgc gaatgatctc gtactcgtgc
     9661 acgagctcgt agtcggtgac cgatttcaga gcgtgcatga agacagggtt caccccgtcg
     9721 aagatgatca cgcgttcggt gatctcggac gcgtcggccg ccaccaccgg cgcctccggc
     9781 gcggagcggg cgacggtctg cgagaacagc gccgccagga gggtcatgaa caagatgggg
     9841 cggacgccgc ggcgtcgccg tgtggggctc gagatcacgg tgcttcctcc ggattcagag
     9901 ggtccaggcc gcgtcattcc cctgacgatc cgcgaggcga aggccgggtg ggccgtgccg
     9961 gtccgcggtg ccggggcccc tggatgcggc cggccctggt cggttgcttg gtggacactt
    10021 cgcgcccgaa atgcgaaatc ctgcccggaa tccgcgtgtg tcggcatctg tgacctttgc
    10081 gcaacctgaa tgttccgggg agcggaacga gatgcggcta tttgggcccg cgacgcgacg
    10141 caaggcatag actggcgata cagacccgag atcggaggcg cgtggtgtca cccctgggac
    10201 gaatgatcac ggccatggct acgccgttcg cgaaggacgg ctccctcgat ctcgacgggg
    10261 ccgtcaagct tgcctcgcat ctggccgacc acggctccga cggcgtcgtt ctggcgggca
    10321 cgaccggcga gtcgcccacc ctgaccaggg aagagaagcg cgagctgttc tcggccgtgg
    10381 ttcccgcggt tcgaggccgc gcctccgtca tcgccgggac cggcacctat tcgacggcgg
    10441 agtccgttga gctttcgcgc atggcggccg gctgcggcgt ggatgcgctt ctggtcgtga
    10501 ccccctacta ctcccgcccg ccgcaggccg ggctcgaagc gcacttccgg accgtggccg
    10561 ccgcgaccga tcttccggtg atgctctacg acatcccggc gcgcaccgcc cgcaagatcg
    10621 agcacgagac cttcctgcgc ctggcagacg tcccgggcat cgccggcgtc aaggatgcgg
    10681 ccggcgatgc ccgggacgtc tgccaggcgc aggaaggtct cgtgctcgat cttgcgggcg
    10741 gtgcgcgccg ggatgt
//
LOCUS       DATLFO010000087         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_52307, whole genome shotgun sequence.
ACCESSION   DATLFO010000087 DATLFO010000000
VERSION     DATLFO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52307"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VM841_02930"
     CDS             <1..460
                     /locus_tag="VM841_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89169.1"
                     /translation="TGAAKPPCREGSVRKLDNDFVAHDTLLAGTVRARALRSGNTVDV
                     PPDDPAEILRLAAGMSDEAPLEVEVEIPLRLVTVVSSRREVCRGGTWIPEVETATHDG
                     PPRVERLRAIVTGPDDLERFAREILAKRLRTLSAARRRLDAALDAMRGRG"
     gene            487..1077
                     /locus_tag="VM841_02935"
     CDS             487..1077
                     /locus_tag="VM841_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HVL89170.1"
                     /translation="MEFQRVVERRRMVRNFEDRPVSEAAVERILGNAIRAPSAGFSQG
                     WGFLVLTDPEDRARFWEVAWPDDERTGSPHEGVMRAGLVVVPCANKQAYLDRYAEPDK
                     GWTDRDERHWPVPYWLVDTSFATMAMLLTITDEGLGALFFSLRHGDAVKDAFGIPPEI
                     EPIGALAAGYPARDEPSPSLARGRRPGSEVIRRRRW"
     gene            1731..2414
                     /locus_tag="VM841_02940"
     CDS             1731..2414
                     /locus_tag="VM841_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89171.1"
                     /translation="MRVLVVDDEEAVARAVRHALEHEGYQVSVCDNGASAQKLARAWR
                     PDVILLDLGLPDMDGHEVAQSIRADTGAPIIMVTGRGEESERIVGLELGADDYVVKPF
                     SIPELVARVRAVVRRASAKPVLQAESLQFRDLKLDTATYRCFLGDQEIALTQKEFEVL
                     RLLMHRPGAAVRRDEIIRAVWNATPAEAAKTLDVHMSSLRKKLGDDPKDPRYIETVHG
                     VGFRLVQGQ"
     gene            2429..2569
                     /locus_tag="VM841_02945"
     CDS             2429..2569
                     /locus_tag="VM841_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89172.1"
                     /translation="MGYVKDFFTNLKNAEGRVGAKLRTTAKNRVRALPRGCCGNYGEP
                     GC"
BASE COUNT          406 a          888 c          918 g          429 t
ORIGIN      
        1 gaccggcgct gcgaagccgc catgccgcga gggatcggtc cggaagctcg acaacgattt
       61 cgtcgcgcac gacacgctcc tcgccggcac cgtccgcgcg cgagcgctgc gcagcggcaa
      121 caccgtggac gtgccgcccg acgatccggc cgagatcctg cggctcgccg ccggcatgtc
      181 cgacgaggcc ccactcgagg tcgaggtcga gatcccgctt cggctcgtga cggtcgtgtc
      241 ctcgcgccgc gaggtctgcc gcggcggcac ctggatcccc gaggtcgaga ccgccaccca
      301 tgacggcccg ccccgcgtcg aacggctgcg cgcgatcgtg accggcccgg acgatctcga
      361 gcggttcgcg cgtgagattc tcgccaagcg gttgcggacc ttgtcggccg cgcgccggcg
      421 gctcgacgcc gcgctggacg cgatgcgcgg ccggggctga cggatgcggt gaccgaggag
      481 gcgaggatgg aatttcaacg cgtcgtcgag cgtcgccgga tggtgcgcaa cttcgaggac
      541 cggccggtga gcgaggccgc ggtggagcga atcctcggca acgcgatccg tgcgccgtcc
      601 gccggcttct cgcagggatg gggattcctc gtgctgaccg acccagagga ccgcgctcgc
      661 ttctgggagg tcgcctggcc cgacgacgag cggacgggat cgcctcatga gggcgtgatg
      721 cgggccggtc tcgtcgtcgt gccttgcgcg aacaagcagg cgtacctgga ccggtatgcc
      781 gagcccgaca aaggatggac cgatcgggac gagcgccact ggccggtgcc gtactggctc
      841 gtggacacct cgttcgcgac gatggccatg cttctgacga tcaccgacga agggctcggg
      901 gcgctgttct tcagcctgcg tcacggggac gcggtcaaag atgccttcgg aattccgccc
      961 gagatcgagc ctatcggcgc gctcgctgcc ggctaccccg cccgcgacga gccttccccg
     1021 tcgctggcgc gtggccgtcg tcctggctcc gaggtgatcc ggcggcgacg ctggtgacct
     1081 tctcgtggcc ccgtttcgtg gcggctagca gacgctcgag attggcgacg agctccgaat
     1141 agcggcgctc gagctcctcg atcgacagcg atcgcgcacc cacgcccagc gcggttcccc
     1201 ctgcggcggc acgatccacg tcctcgctgt gaacgaacac cccgcggatg ccgggccggc
     1261 gtgtggtgcg aaccagcccg cgccgttccc aatgacggat cgtgttgtag tgaacgctcg
     1321 cccgctcgga tgcctgctct cgggtgatcc acgggccgcc cggtgcctgc ggcttgggtc
     1381 gtgcgagcgc cgcgcgcttc ggagtcacgc cgtgccgggc ggaacggtga cgacccagcc
     1441 ccatcgcgtg cttggcaacg aagtcgcagt cggggcacgg aagcccgccg gccacttttt
     1501 tcgttgcgcc ggacggtcgc ttcccggcct tcttcttggc tcccacggcc ccctccatgt
     1561 catgtcgtcg gtctgcgttg tcggatgcgg ctgatgctaa ccgattttct taacctgctt
     1621 tcaaatccga ggagaatgcc ctggtagtga cgccgccgtg ggggcattca ataagatggg
     1681 acgagcaaac gacgggggcc gacgtggacg cattcggaag cgggggacca ttgcgcgttc
     1741 tcgtcgtcga cgacgaagaa gccgtcgcgc gggcggtgcg gcacgcgctc gagcacgagg
     1801 gttatcaggt ttcggtctgc gacaacggcg cgtccgcaca gaagctcgcg cgcgcatggc
     1861 ggcccgacgt catcctgctc gacctcggct taccggacat ggacggccac gaggtcgcgc
     1921 agagcattcg agccgacacg ggcgcgccga tcatcatggt caccggccgc ggcgaggagt
     1981 ccgagcgcat cgtggggctc gaactcgggg ccgacgacta cgtggtcaag ccgttctcga
     2041 ttcccgagct cgtggcgcgg gtgcgtgcgg tcgtgcgccg cgcgtccgca aagcccgtat
     2101 tgcaggcgga atcgctgcaa tttcgggatc tgaagctcga tacggccacc tatcgttgct
     2161 ttctgggcga ccaggagatc gccttgaccc agaaggagtt cgaggtcctt cggttgctga
     2221 tgcaccgtcc gggcgcggcg gtacggcgcg acgagatcat ccgggcggtc tggaacgcca
     2281 cgccggccga ggcggccaag acgctcgacg ttcacatgtc gtcgctgcgc aagaagctcg
     2341 gcgacgatcc gaaagacccg cgatatatcg agaccgtgca cggtgtcggc ttccggctcg
     2401 tgcagggaca gtgaccgagg agggcccgat gggttacgtg aaagattttt tcaccaacct
     2461 caagaacgcc gaggggcgcg tcggtgcgaa gcttcgcacc acggctaaga accgcgtgcg
     2521 agctctgccg cgaggctgct gcggcaacta cggcgagccc ggctgctgac gggccgcgcg
     2581 cccggagccc ggtcgggact ccaacgatct tccccacggc gatcatcccc acggcgatca
     2641 t
//
LOCUS       DATLFO010000088         4068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_52314, whole genome shotgun sequence.
ACCESSION   DATLFO010000088 DATLFO010000000
VERSION     DATLFO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52314"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..599
                     /locus_tag="VM841_02950"
     CDS             156..599
                     /locus_tag="VM841_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVL89173.1"
                     /translation="MLRDRIGEDLKDAMKARDQVRVATLRMLIAAVKNAQVEKMHELD
                     DDEVIEVATREAKRRKESIEAFRSGGRDDLVTKEEAELAVLQQYLPEQLGDDELAALV
                     DDAIAETGATGPKEMGAVMKALMPKVRGRADGAAVSAMVKQRLSG"
     gene            complement(596..2938)
                     /locus_tag="VM841_02955"
     CDS             complement(596..2938)
                     /locus_tag="VM841_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HVL89174.1"
                     /translation="MSDSSIRGEYDPERIRRAKSFLQRIRDSAMGDFDHDIPPPPERV
                     DRAYRFALASIVIVLASAMIAVMALPGSAFLGRMVGIVADRLVVAGPGGKIEAPRIAQ
                     RSVIVGAEGGIVATLAGEENRRVVSLPQIAPIARNAVLAIEDARFFHHSGIDGAGLVR
                     AVFANARAGQVREGGSTITQQLVKNVFVGNERTLERKLREAQYAVALEKEMTKSQILE
                     LYLNETYFGDGHYGIETAAEYFFGTPASKLDAVQSALLAGLIAAPERYNPFEFPERAK
                     ARRNTVLGRMAAEGFIERADLEKLKAAPLGVEKHPLPPAKEPYFVDFIKRQILDDPRF
                     GATRSERAKALFQGGLRIETTLNLRLQDSARQAVDTVLDEDGDPAAALVSVEPATGAV
                     RAMVGGRDFNSEQFNLAIGTNRQAGSTFKPFVMAAALDQGISPGLTLDTPSPYRTTDD
                     MGKPWAPANYSNRGEGLMNMRKATELSVNTYYVQLIELVGPDKVVDMAKRLGITSPLE
                     PYKSLSLGTFGVSPYEMASAYGTLANNGIACKPYTIRRVLAADGTEILRNDPSCTRVV
                     DATIAATAVDILKGVIDRGTGRRNGQIGRPAAGKTGTTDDYTDAWFMGFTPQFSTAVW
                     LGFPDSTERGLYNIHGLPQVFGGSLPAEIWNKFMKVAHDGLPAVAFPEPPTVEFTTVP
                     TLVGLHRDDAMTQLKSAGLNVRTRIVSSSLSPGIVVAQLPSPGTEVEKGSLVEIQVSD
                     GSEATSPPEPQDPTPEPYPSPTPSSSPNSTNDAETQERGR"
     gene            complement(2966..>4068)
                     /locus_tag="VM841_02960"
     CDS             complement(2966..>4068)
                     /locus_tag="VM841_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HVL89175.1"
                     /translation="EQHVIVCTGPGGVGKTTTAASLALRAADEGRTVIVCTIDPAKRL
                     AQSLGLEELDNEPKPVPAKAFKGRKPKGELWAMMLDTKRTFDEMVLDMTTPDRAEKIF
                     ENSFYQHISSSLSGTQEYMAMEKLFELHEEGRWSLIVIDTPPTRNALDFLDAPRKLTD
                     FLDSRFLRALLWPTIQAGKSTFRAFNMGAKVVLRAATRITGSELLSDVASFFAAFDGM
                     YGSFKHRAERVREMLSAKRTSFVVVAAPQVASLREARFFVDRLRAEQIPLGGVVINRT
                     HLAPPVLDDPRELAERATKSGEAPLLAAALEVYASWQEIAEREARAIEDALGGAQLPP
                     LWGVPDFADEVHSVPALRRLSDHLLGPVVPSD"
BASE COUNT          648 a         1347 c         1434 g          639 t
ORIGIN      
        1 gaccatcagg ggctcggagc cgggtggcag gcaggcgatc cggtggcggc gcaagacgaa
       61 ccacgacgct tcgaggcgag cgtagacgag tccggcgatg ccggcggcgg ccaggccgcc
      121 gatgatccac cacatggggg cggtattgta cggccatgct gcgagaccgg atcggcgagg
      181 acctcaagga tgcgatgaag gcacgcgatc aggtgcgggt ggcgacgctg cgcatgctga
      241 tcgccgcggt caagaacgcc caagtcgaga agatgcacga gctcgacgac gacgaggtga
      301 tcgaggtcgc gacgcgcgaa gccaaacgcc gcaaggaatc gatcgaggca ttccgatccg
      361 gaggccgcga cgacctcgtc accaaggaag aggccgagct cgcggtgctg cagcagtacc
      421 tgcccgagca gctcggcgac gacgagctcg cggcgctcgt cgacgatgcg atcgccgaga
      481 ccggcgcgac cgggccgaag gagatgggcg cggtcatgaa ggcgctgatg cccaaggtgc
      541 gcggccgcgc ggacggcgcc gccgtctcgg cgatggtcaa gcaacggctc agcggctagc
      601 ggccgcgttc ctgcgtttcg gcgtcgttgg tgctgttcgg cgaggaggac ggggtcggcg
      661 acggatacgg ctcgggcgtg ggatcctgcg gctccggcgg cgacgtcgcc tcgctgccgt
      721 ccgagacctg gatctcgacc agcgatccct tctcgacctc cgtgccgggc gacggaagct
      781 gcgcgaccac gatacccggc gacagcgacg aggagacgat gcgcgtgcgc acgttcaacc
      841 ctgccgactt cagctgagtc atcgcgtcgt cgcgatgcaa ccccacgagc gtcggcacgg
      901 tcgtgaactc gaccgtgggc ggctcgggga acgcgacggc cggcaggccg tcgtgcgcga
      961 ccttcatgaa cttgttccag atctcggcgg gaaggctgcc gccgaacacc tgcggcagcc
     1021 cgtgaatgtt gtagaggccg cgctcggtcg agtcggggaa gcccagccag accgcggtcg
     1081 agaactgcgg cgtgaagccc atgaaccagg cgtcggtgta gtcgtcggtc gttccggtct
     1141 tgccggcggc cggccggccg atctggccgt tgcggcgccc ggttccgcgg tcgatcacgc
     1201 ccttgaggat gtccacggcc gtcgcggcga tcgtcgcgtc caccacgcgc gtgcaggacg
     1261 gatcattgcg aaggatctcg gtgccgtcgg cggcgagcac gcggcgaatc gtgtacggct
     1321 tgcaggcgat gccgttgttg gcgagcgtgc cgtacgccga ggccatctcg tagggcgaga
     1381 ccccgaacgt gccgagcgaa agcgacttgt acggctcgag cggcgaggtg atgccgagcc
     1441 gcttggccat gtccacgacc ttgtcgggac cgaccagctc gatcagctgc acgtagtagg
     1501 tgttgaccga cagctcggtg gccttgcgca tgttcatcag gccctcgccg cggttcgagt
     1561 agttcgcggg cgcccacggc ttgcccatat cgtcggtcgt ccggtacggc gaaggcgtgt
     1621 cgagcgtgag gccgggcgag atgccttggt cgagcgccgc ggccatgacg aacggcttga
     1681 aggtcgagcc cgcctgccgg ttggtgccga tcgccaagtt gaactgctcg gagttgaagt
     1741 cccggccgcc gaccatcgcg cgcaccgcgc ccgtcgcggg ctcgaccgac accagcgccg
     1801 ccgccggatc gccgtcctcg tccaggaccg tgtccacggc ctgacgcgcc gaatcctgca
     1861 gacgaaggtt gagcgttgtc tcgatgcgca agccgccttg gaacagcgcc ttcgcacgtt
     1921 ccgaccgcgt cgcgccgaag cgcgggtcgt cgaggatctg ccgcttgatg aagtccacga
     1981 agtacggttc cttcgccggc ggcagcgggt gcttctcgac acccagcggc gcggctttga
     2041 gcttttcgag atcggcgcgc tcgatgaacc cttcggcggc catgcgtccg agcaccgtgt
     2101 tgcggcgcgc cttcgcgcgc tcggggaact cgaacgggtt gtagcgctcg ggcgcggcga
     2161 tgaggcccgc aagcagcgcc gactgcaccg catcgagctt cgaggcggga gtcccgaaga
     2221 agtactcggc cgccgtttcg attccgtagt ggccgtctcc gaagtaggtc tcgttcaggt
     2281 acagctccag gatctgcgac ttggtcatct ctttctcgag cgccaccgcg tactgcgcct
     2341 cgcgcagctt gcgttcgagc gtgcgctcgt tgcccacgaa cacgttcttg acgagctgct
     2401 gcgtgatcgt cgagccgccc tcgcgcactt gtcctgcgcg cgcgttcgcg aagaccgccc
     2461 gcaccagtcc ggcaccgtcg atgccgctgt gatggaagaa gcgcgcgtcc tcgatcgcga
     2521 gcaccgcgtt gcgggcgatg ggagcgatct ggggcagcga gaccacacgc cggttctcct
     2581 cgcccgccag cgtggcgacg atgccaccct cggcgcccac gatgaccgag cgctgcgcga
     2641 tgcggggcgc ctcgatcttg ccgcccggcc ccgcgacgac gagacggtct gccacgatgc
     2701 cgaccatccg gccgaggaac gccgagccgg gcagcgccat caccgcgatc atcgccgagg
     2761 cgagcacgat gacgatcgag gccagcgcga accgatacgc gcgatccacg cgctccggtg
     2821 gcggcgggat gtcgtggtca aagtcgccca tcgcggagtc cctgatgcgc tgcaagaacg
     2881 actttgcgcg ccggatccgc tccgggtcgt actccccccg tatcgaagaa tcgctcatcc
     2941 ggggaaagtg taccggtccg tctccctaat cggaggggac gacaggtccg agcaaatggt
     3001 ccgaaaggcg gcgaagcgca ggcaccgaat ggacctcgtc ggcgaagtcg gggactcccc
     3061 acaggggcgg aagctgcgcg ccgcccagcg cgtcctcgat cgcgcgtgcc tcgcgctccg
     3121 cgatctcttg ccacgaagcg tagacctcga gggcggcggc gagcagcggc gcctcgccgc
     3181 tcttggtggc gcgctcggcc agctcgcgcg gatcgtccag gaccggcggt gcgagatggg
     3241 tccggttgat cactacgccg ccgagcggga tctgctccgc gcgcagtcgg tccacgaaga
     3301 agcgcgcctc gcgcagcgag gcgacctgcg gggccgcgac caccacgaac gaggtccgct
     3361 tggcgctgag catctcgcgc acccgctcgg cgcgatgttt gaacgagccg tacatgccgt
     3421 cgaaggcggc gaaaaagctc gcgacgtcgg agagcagctc ggatccggtg atgcgcgtcg
     3481 cggcgcgcag cacgaccttg gcgcccatgt tgaacgcgcg gaaagtggac ttgcccgcct
     3541 ggatcgtcgg ccacagcagc gcgcgcagga accgggagtc gaggaagtcg gtcagcttcc
     3601 gcggcgcgtc gaggaagtcg agcgcgttgc gggtcggagg cgtgtcgatc acgatcaggc
     3661 tccagcggcc ctcctcgtga agctcgaaga gcttctccat cgccatgtat tcctgggtgc
     3721 cggacagcga cgaggagatg tgctgataga aggagttctc gaagatcttt tcggcccggt
     3781 cgggcgtcgt catgtcgagc accatctcgt cgaaggtgcg cttcgtgtcg agcatcatcg
     3841 cccacagctc gcccttgggc tttcggccct tgaacgcttt cgccggcacc ggcttcggct
     3901 cgttgtcgag ctcttcgagc ccgagcgact gggcgagccg cttcgccgga tcgatcgtgc
     3961 acacaatgac ggtgcgtcct tcgtcggccg cccgcagcgc gagcgaggcc gcggtggtgg
     4021 tcttgccgac tccgccgggg ccggtgcaca cgatcacgtg ctgctcgc
//
LOCUS       DATLFO010000089         9923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_111359, whole genome shotgun sequence.
ACCESSION   DATLFO010000089 DATLFO010000000
VERSION     DATLFO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111359"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VM841_02965"
     CDS             <1..628
                     /locus_tag="VM841_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89176.1"
                     /translation="CACRSMADRAVARTLLDRERMLDESQRATALSAIERHAHGLMDF
                     SRDIMEILQAEEGALPLTRAPLDLGGAAAAALERIRKDFPDHRFGLDVERTHLTVCAD
                     PRRIGQVIENLLGNAAKYSPPGTLVSVTVRRGPDHVVVAVSDAGPPLPGDIVAAPFTR
                     RGPRDDSGGGNGLGLYLCRVLVESMGGRIWAENRPGMVVVGFLLPVSG"
     gene            complement(551..4321)
                     /locus_tag="VM841_02970"
     CDS             complement(551..4321)
                     /locus_tag="VM841_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL89177.1"
                     /translation="MAGVCAVRLGSSGLVLGKGSPTTVALLVPSGSLRAAAVPAAPVA
                     ALTDNKVVLALTVFALLVLLIRLVLKVSESMSLTRTLERRVEERTTDLELALRDREAQ
                     RRALAESEERFRMLAETASDIVFRFRIAPTPGFEYLSPSVERVTGYTVEELYKDPTIS
                     WARIVEDLDLVYRSIQDAANESVELRWRRKDDRTIWIELRTAVVRENGEPIAFQGIAR
                     DITSRKKAEAERTALAAIVESSAHGIVSVDLDGRFISWNHGAEQLLGYSAQEIIGKPL
                     MWLVPPDRVSEVMDSRRRCTEGESVGPFSTQRIHKDGRLVDVSITLSPLVDESGSVTG
                     TSAIMQDIGDRLRAEQQLRVSEARSRIILEAANTALIGCDPNGRVAEWNRRAEGLLGY
                     SAGEALGRHVDFLFPPEHVATQRNALSEIMRTGKGPGVGRPAPLTAVRKDGRRVRLEA
                     HVWPVEVENTRRVYAFLFDVTERTRAERALRRSEETFRSLLQTAPDAAVIVDSRKRIV
                     LINTAAENMFGYPLDRLLGEPVETLLPDGLPATKAATEQTGRRRDEQTFPVEVRLSFV
                     EAGDEVFTAAYVRDITERKLVEEALRRSEKRFRSLLQSAPDAAIIIDGNGRIVLANTE
                     AERLYGYEPGDLVGRDVAILVPERFRVRHGMGFFEVARNAQVDEGQQLSLGHARDVFA
                     RRKDGSEFPVDASLSAFETDDGVFLTALIRDVTEQREIEMMLRRSERRFRAIFEEGPV
                     GVLVIAADDTIVRANPALNRMLGYDKEALLGMRLRDITYGPDRGIGSRDRALMREGGL
                     DTTQIRKRFLRKDGSVIWVDLSWSKLTEDGATLLVGLVQDVTTQREAEDALRKANDEL
                     RRASEAKSVFLARMSHELRTPLSAILISAEMLTTPAYGARSEEMLTQLGNRILQGGRH
                     LLGLIDDLLDLTRIETGRLQITPVPVSAAGLMSELEGALGPHARRKQLRLELPAAEGI
                     TILADPLRLRQVFLNLIGNALKFTDAGGHVWVGITQRRGLVTISVADTGPGIAPEDLG
                     RIFEPFEQASHRNEGAGLGLAISKQIVEMHGGTIKVDSRPGKGSIFLVTLAGVNADST
                     ETDEPDASERSAARAGTILLVEDDEDIASLMLDVLRTAGYKPVHAATTSGAVEAAAAS
                     RPDLVLLDIGLGAESGLDIIDDLRSLPGGQATRILALSAYAMPDEIKRAKDAGCDDYL
                     VKPLGAGDILARIAHWLAIGSGDNRAGSAGNGQEKADDDHAGAILGPDSSAH"
     gene            complement(4470..4823)
                     /locus_tag="VM841_02975"
     CDS             complement(4470..4823)
                     /locus_tag="VM841_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL89178.1"
                     /translation="MDDDGLHRGALIDALIFSGFSVAEAATGGEGIDAAAAGGVDAVL
                     LDIGLPDVDGVDLIPRLREAARFAGLPVIALSGLTRAADRDRAFKAGCNAYMTKPPSV
                     RELIGTLSILCLEGD"
     gene            complement(4856..5497)
                     /locus_tag="VM841_02980"
     CDS             complement(4856..5497)
                     /locus_tag="VM841_02980"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVL89179.1"
                     /translation="MRERRGFDPLLDLARMIEARGADGDGHADRLARSAEMLARATGM
                     PETDVAAVKLGAHLHDIGKVMVPEWIIWKTGPLTDDETTVMRGHPIVGAQMLTFTGVP
                     AGAREIVLRHHERFDGTGYPMGLTGPKLPVTSRIVTIADAYDAMRSNRPYRRALSRDE
                     AIRRLRDGAGRQFDPDLVEPFCALAIPEPPIDEEPIWQRMKSVQSGGGRSHAL"
     gene            complement(5553..6128)
                     /locus_tag="VM841_02985"
     CDS             complement(5553..6128)
                     /locus_tag="VM841_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89180.1"
                     /translation="MRITGSCSHVAEAVEATRASVAAAVLFGAQQPDETSSIKALAAA
                     GPSAAIVVVSAWIDGRSIVTALRAGARGYMPEWASPDEIVDALAAAVDGALSVHPAIA
                     VAGLAFAVERLDGPSEPEPLSRLTARERRVLDLLEDGMSPAEIASRLFLSKRTIQSHL
                     QGAYSKLGVHGRVEAMWEYRRLRGHVGEHVS"
     gene            complement(6315..7037)
                     /locus_tag="VM841_02990"
     CDS             complement(6315..7037)
                     /locus_tag="VM841_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89181.1"
                     /translation="MRPNPETIPETSDRAQDPDDDADTAGTRVLIVDDHAAFAEPLAA
                     VLGLQNDMDCAGIAGRCDEAVKMAEDLRPDVVLMDVQLPDADGIEGSRKIKEVSPHSR
                     VLILTAYSDVEVMARAAAAGAAGFIPKETPLADILHAIRTAREGGMLVEGNSLSALLA
                     HIRSVDEPRPSPRAVDLTPREFEVLTLMGEGKDAQTIARLLGITVLTCRGHIKKIHSK
                     LGVHSQLEAVVTAMRMGILSAP"
     gene            complement(7155..9143)
                     /locus_tag="VM841_02995"
     CDS             complement(7155..9143)
                     /locus_tag="VM841_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89182.1"
                     /translation="MMRRVALGMLAVLVPLLAAGLLPARAASAPEIEPATGRIVVVLL
                     NGTSLEDWLRADTFHLRRAIREGTPGLLSARTAAKPPAGERECLARPGHAMATLMNGT
                     RSRASVSGGAVPLREALRRAETTLRLLDTPVVRDSSFPTGRRADQSRLARQYETAAEG
                     VVIVDIPDTLYADCAGGRQRDRWVSVALGRADATIGVVRSSLEQGDRMLVMSAVPPAA
                     REDRRNYLAAATLENPVRDDAAILTSPTTRRRGVVSLTDAASTIVALAGGDATVGVGR
                     RWGSEPGTPEYLRRADRDFTHAASIRLPVMRGYVWLAAGLIVVAAVVVLAGRGRARGG
                     RLPRTGRDWLATLLVALVAAPAAMLIEPLTGVTTLAASVAVITSASLGLALVAGAVRR
                     IPFAAGVCAGIAASGLIAFVGQGELGARSALSYSIAGGDRFFGVGNEMMGVAVAGALL
                     ATGQWLDARRDALKWAPPLLLLTAGAMAAPSAGAKFGSIFVAVPAFGLFLALAAGRRP
                     SRAAALGIAIATVFLAGIFAAWDLLADPASQSHVARVVADDDTLGRKIGAALRLLAGS
                     YWTIGLVVASGVALLFVRRRTTLLARATWARPHHRAALIGCGVAAVAAVVFNDAGIVA
                     AALIAIVAAASLLHTILVGQSPPVVATESIKRLREFAG"
     gene            9180..>9923
                     /locus_tag="VM841_03000"
     CDS             9180..>9923
                     /locus_tag="VM841_03000"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HVL89183.1"
                     /translation="MTAWDLDDGVLREVLSEALATGGDFAEIFAENRDSMSVRLEDRK
                     VEELVAGRDRGAGIRVVKGESTAFAFTNRLDRGALVDAAKAAAAGLTDQRLVDVQDLR
                     REPETVLNPVRIDAREVPADRKVAYVVALDEAARAEGGGEIRQVIGSYVEGSQRVLIA
                     NSDGLIVRDNRTRTRVSCQVVAARGDVIQTGFHSPGASAGFEHLDAHPADFVGRFAAR
                     QAIAMLGSIPAPPGASTTGISPAGGGGVLF"
BASE COUNT         1546 a         3415 c         3402 g         1560 t
ORIGIN      
        1 gtgtgcgtgc cgctcgatgg ccgacagagc agtcgcgcgc acgctcctcg atcgagagcg
       61 catgctcgac gagagccagc gcgcgactgc tctgtcggcc atcgagcggc acgcacacgg
      121 gctgatggac ttctcgcgcg acatcatgga gatcctgcaa gccgaggagg gagcgctgcc
      181 gctcacgcgc gcccccctcg atctaggtgg ggctgcggcc gctgcgctcg agcggatccg
      241 taaggacttc cccgatcacc gcttcggtct cgacgtagaa cgcacacact tgacggtgtg
      301 cgccgatccg cggcgcatcg gtcaggtgat cgaaaatctg ctcggcaacg ctgcgaaata
      361 ctcgccgccg ggaacgctgg tctcggtgac ggttcgccgc ggacccgacc acgtcgtggt
      421 cgcggtctcc gatgccgggc cgccactgcc cggcgacatc gtcgcggcgc cgttcacgcg
      481 ccgcggtccg cgcgacgact cgggcggcgg caacggcctc gggctctacc tgtgccgggt
      541 gctggtcgag tcaatgggcg gacgaatctg ggccgagaat cgccccggca tggtcgtcgt
      601 cggctttctc ctgcccgttt ccggctgagc ccgccctgtt gtcgccgctg ccgatcgcga
      661 gccagtgcgc gatacgcgcg aggatgtcgc ccgcccccag gggcttgacc aagtagtcgt
      721 cgcagccggc atccttcgcc cgcttgatct cgtcgggcat cgcgtacgcg ctgagcgcca
      781 ggatgcgcgt ggcctgtccg cccggcaatg agcgaaggtc gtcgatgatg tccaggccgc
      841 tctcggcgcc cagaccgatg tcgagcagca ccagatcggg ccggctcgcg gcggccgcct
      901 cgaccgcccc ggacgtggtt gcggcatgca cgggcttgta tccggccgtt cgcagcacgt
      961 cgagcatgag cgaggcgatg tcttcgtcgt cctccacgag aaggatcgtt cctgcccgag
     1021 cggccgagcg ctccgatgcg tccggctcat cggtttcggt cgagtccgca ttcactcctg
     1081 cgagcgtgac caggaagatc gatcccttgc cgggacggct gtcgactttg atcgtgccgc
     1141 cgtgcatctc cacgatctgc ttcgagatcg caaggcccag tcccgcgcct tcgttccggt
     1201 ggctggcctg ctcgaacggc tcgaagatcc ggccgagatc ttcgggggcg atgcccggcc
     1261 cggtgtcggc gaccgagatc gtcaccaacc ctcttcgttg ggtgatcccg acccagacgt
     1321 gcccgcccgc atccgtgaac ttgagcgcgt tgccgattag gttcaggaac acctgccgca
     1381 gcctgagcgg atccgccagg atggtgatgc cttcggcggc cggaagctcc agacgcagct
     1441 gtttgcgccg cgcgtgcggg ccgagcgcgc cttcgagctc gctcatcagt cctgcggccg
     1501 ataccggcac gggggtgatc tgcagccgtc ctgtttcgat gcgcgtcaga tcgagcagat
     1561 cgtcgataag gccgagcagg tgtcgtccgc cctgcaggat ccggttcccg agctgcgtga
     1621 gcatctcctc ggaccgggcc ccgtaggcag gagtggtgag catctcggcc gagatcagga
     1681 tcgccgacag cggcgtgcgc agttcgtgcg acatgcgcgc cagaaagacc gacttcgcct
     1741 cactcgctcg ccgcagctcg tcgttcgcct tgcgcagcgc gtcctcggcc tcgcgctggg
     1801 tcgtgacgtc ctggacgagg ccgacgagca gcgtcgcgcc gtcctcggtg agcttggacc
     1861 acgaaagatc gacccagatg accgaaccgt ctttccgcag gaagcgcttg cggatttggg
     1921 tcgtgtcgag gcctccttca cgcatgagcg cgcggtcccg ggatccgatg ccgcggtcgg
     1981 gcccgtacgt gatatcacgc aagcgcatgc cgagcagcgc ctccttgtcg tacccgagca
     2041 tgcggttcaa cgccgggttg gcgcgcacga tcgtgtcgtc ggcggcgatg acgaggacgc
     2101 cgaccggacc ctcctcgaag atggcgcgga agcgacgctc gctcctgcgg agcatcatct
     2161 cgatctcgcg ttgctcggtc acgtcgcgga tcagcgccgt cagaaagacg ccgtcgtcgg
     2221 tctcgaatgc ggacaggctc gcgtcgaccg ggaactcgga tccgtccttt cggcgggcga
     2281 acacgtcgcg ggcatgtccc agcgagagct gctgtccctc gtccacctga gcattccgag
     2341 cgacctcgaa gaatcccatg ccgtgccgga cgcggaatcg ctcgggtacc aggatcgcga
     2401 cgtcgcgacc gaccagatcg cccggctcgt agccgtagag ccgctccgcc tcggtattgg
     2461 cgagcacgat gcggccgttt ccgtcgatga tgatggccgc gtcgggcgcc gactggagca
     2521 acgagcgaaa gcgcttctcg cttcgtctca gcgcctcctc gaccagcttg cgctcggtga
     2581 tgtcgcgaac gtaggccgcc gtgaagacct cgtccccggc ctccacgaac gacaggcgca
     2641 cctcgaccgg aaacgtctgc tcgtcccggc gccggccggt ctgctcggtg gccgccttcg
     2701 tggccggcaa cccgtccggc agcaacgttt cgacgggctc tccgagcagc cgatcaagcg
     2761 ggtatccgaa catgttctct gcggcggtgt tgatcagaac gatgcgcttg cgagagtcga
     2821 cgatgactgc cgcgtcggga gcggtttgca gcaggcttcg gaaggtctct tcgctgcgac
     2881 gcagcgcccg ttccgcgcgg gtccgctcgg taacgtcgaa caggaaggcg tagacgcgtc
     2941 tggtgttttc gacctcgacg ggccagacgt gcgcctcgag ccgcacccgg cgtccgtcct
     3001 tgcggacggc ggtcagcggc gccggacggc ccacgcccgg gcccttgccg gtccgcatga
     3061 tctccgagag cgcgttgcgc tgggtagcga cgtgctcggg cgggaacagg aaatccacat
     3121 gacgtccgag cgcctcgccg gcgctgtagc cgagaaggcc ctcggctcgc cggttccatt
     3181 cggcgacacg gccgttcgga tcgcagccga tcagggcggt gttggcggcc tcgaggatga
     3241 tccggcttcg agcctcgctc acgcgcagct gctgctcggc gcgcagccgg tcgccgatgt
     3301 cctgcatgat ggccgacgtc cccgtgacgg agccgctttc gtccacgagc ggggacaggg
     3361 tgatcgacac atccacgagg cgtccgtcct tgtggatgcg ctgcgtcgag aaggggccga
     3421 ccgactcgcc ctccgtgcag cgccggcggc tgtccatcac ctcggagaca cggtccggcg
     3481 gaaccagcca catcagtggc ttgccgatga tctcctgggc ggaatagccg agcaactgct
     3541 cggccccgtg attccacgag atgaaccgtc cgtcgaggtc cacgctgacg atgccgtgcg
     3601 ccgaggattc gacgattgcc gcaagcgccg ttcgctccgc ttcggctttc ttgcgtgacg
     3661 tgatgtcacg cgcgattcct tggaaagcga tcggctcgcc gttctcgcgc acgacggcgg
     3721 tccgcagctc gatccatatg gtgcggtcgt ccttgcggcg ccaccgcagc tcgacggatt
     3781 cgttcgctgc gtcttggatc gatcgataca cgagatcgag atcctccacg atacgcgccc
     3841 acgagatcgt cggatccttg tacagctctt cgaccgtgta tccggtgacg cgctccaccg
     3901 aagggctcag gtattcaaag ccgggcgtgg gagcgatgcg gaaccggaag acgatgtcgc
     3961 tcgccgtctc cgcgagcatc cggaatcgct cctcgctttc cgcgagcgcg cgtcgctgcg
     4021 cttcacggtc gcggagcgcc aattcaagat cggtggtccg ctcctcgacc cgccgctcga
     4081 gcgtccgcgt cagcgacatg ctttccgaca ccttcaacac cagccggatg agcaggacca
     4141 aaagggcgaa gacggtgagt gcgagcacga ccttgttgtc ggtcagcgcc gcgacgggcg
     4201 cggcaggaac ggcggccgct cgaaggctgc cgctcgggac cagcaaggcg acggtcgttg
     4261 gcgatccctt cccgaggacg aggccggacg aaccgagccg gacggcgcaa acacctgcca
     4321 ccggaccctc cgatgccgtc catactaccc ctgcattcgc gggcgtcgtc ggccgattcc
     4381 gcgaaaggga gccgggcgcg ccgttccccg acgacgggcg tcggagttgg tcgaggagac
     4441 ggcgcctgcc gcggctcact tgcctgtcct cagtctcctt cgaggcacaa gatggagagg
     4501 gttccgatca gctcccggac gctcggcggc ttcgtcatat aggcgttaca ccccgctttg
     4561 aacgctcgat cccggtcggc tgcgcgagtc agaccgctga gcgcgattac gggcagaccc
     4621 gcgaaacggg ccgcttcccg cagacgaggg atcagatcca ccccgtcgac gtcgggcagg
     4681 ccgatgtcga gcagaacggc gtcgacgccc cctgccgctg ccgcgtcgat cccctctcct
     4741 cccgtcgccg cctccgccac cgagaagccc gaaaagatga gcgcatcgat gagggccccc
     4801 cgatgcaggc cgtcgtcgtc cacgacgagg acgcgcggcc gcgactgtgc ttccgtcata
     4861 gagcatggct ccttcccccg cctgattgaa ccgacttcat tcgttgccag atgggttctt
     4921 cgtcgatcgg tggctccggg atcgcgagcg cacagaacgg ctcgaccaga tccgggtcga
     4981 attgtctgcc ggcgccgtca cgcagacgcc ggatcgcctc gtcgcgcgac agcgcgcgcc
     5041 ggtacggccg gttcgatcgc atcgcgtcgt aggcatcagc gatcgtaacg atgcgtgagg
     5101 tcaccggaag tttcgggccg gtcaacccca tcggatagcc ggtgccgtcg aagcgttcgt
     5161 ggtggcgaag cacgatctct cgcgccccgg cgggaacgcc ggtgaacgtg agcatctgtg
     5221 cgccgacgat cggatgaccg cgcatgacgg tcgtctcgtc gtcggtcagc ggtccggtct
     5281 tccagatgat ccattccggc accatcacct tgccgatgtc gtgaaggtgc gcaccgagct
     5341 tgacggcggc gacgtcggtt tcgggcatgc ccgtcgcgcg cgccagcatc tcggcggatc
     5401 gtgcgagacg gtccgcatga ccgtcgccgt ccgcgccgcg tgcctcgatc atccgggcga
     5461 ggtcgagcaa cggatcgaac cctcgtcgct cgcgcacggt gggggggagt cctcccccgg
     5521 cgttgttcgg tgtcgcgccg ggggaggccc cgtcaggaaa catgctcgcc aacatgaccg
     5581 cgtaacctcc ggtactccca catcgcttcg actcgcccgt gcacgccgag cttggagtag
     5641 gcgccttgca gatgcgactg gatggtgcgc ttgctcagaa agagccggct cgcgatctcc
     5701 gcggggctca ttccgtcctc gagcagatcc agcacgcgac gctcgcgcgc ggtgaggcgg
     5761 cttagcggct cgggttcgct cgggccgtcg aggcgctcga ccgcgaacgc gagcccggcg
     5821 acggcgatgg ccggatgcac ggagagcgct ccgtcgacgg cggcggccag cgcatccacg
     5881 atttcgtcgg ggctcgccca ctcgggcatg tagccgcgag cacccgcgcg aagcgcggtc
     5941 acgatcgagc ggccgtcgat ccacgcggaa acgacaacga tggcagcgct gggccctgcg
     6001 gcggccaagg ccttgatgct cgaggtctcg tcgggctgct gcgcgccgaa caggacggcg
     6061 gccgcgacgg aggcgcgggt tgcctcgacg gcctcggcaa cgtgcgagca cgagccggtg
     6121 atgcgcatct gacctgcgtg tctctcgatc gcggccgtga acgcggatcg gcatgcctcg
     6181 tgtctgtcga taatcagaat tccgctcgaa cccataacgc ccacctcgat catgacggtt
     6241 cgtcgagact aggaggatcg aggcagggcc gaaagccccc gttgggtcgc gttcgacgcc
     6301 aaaaacttgg ctctttacgg cgcggagagg atgcccattc gcatcgccgt gacgacggcc
     6361 tcgagctggc tgtgcacgcc gagcttggaa tggatcttct tgatgtggcc gcggcaggtc
     6421 agaacggtga tgcccagcaa tcgcgcgatc gtctgcgcat ccttgccttc tcccatgagc
     6481 gtgagcacct cgaactcacg gggagtgaga tcgacggcgc gaggagacgg gcgcggctcg
     6541 tccacggagc ggatgtgcgc gaggagcgcg gacagagagt tgccttcgac gagcatgccc
     6601 ccctcgcgtg ccgtgcgaat cgcgtgaagg atgtccgcga gcggcgtctc cttcggaatg
     6661 aacccggcgg cgcccgcggc cgcggcgcgg gccatgacct ccacatcgga gtacgcggtc
     6721 aggataagca cccgagagtg gggcgagacc tctttgatct tgcgcgatcc ttcgatgccg
     6781 tccgcgtcgg gaagctgcac gtccatcaac acgacatcgg ggcgaaggtc ttccgccatc
     6841 ttcaccgctt cgtcacaacg gccggcgatt ccggcgcagt ccatgtcgtt ctgcagtccg
     6901 agaacggctg cgagcggttc ggcgaaagcc gcgtggtcgt cgacgatcaa gacgcgtgtg
     6961 ccggccgtat cggcgtcgtc gtcagggtct tgtgcgcggt ccgatgtctc ggggatggtt
     7021 tctggattcg ggcgcatgct gccatggtcg cgtagtgact cgatgggcgt caaggacgcc
     7081 acacacccag agttgggtat gcgcgcgagg cggatgtcat ccggccgatg ccgcggcatt
     7141 cgccggcggc gacgctatcc ggcgaattcg cgcaagcgtt tgatgctctc ggtcgcgacc
     7201 acgggcggag attgaccgac gagaatggtg tgcagcagcg aagcggcggc gacgatggcg
     7261 atcaatgcgg ccgcgacgat cccggcgtcg ttgaagacta cggccgccac ggcagccacg
     7321 ccgcagccga tgagcgccgc ccgatgatgc ggccgtgccc acgtcgctcg cgccagcagc
     7381 gtggtccggc ggcgcacgaa cagcagcgcg acgccggacg cgaccaccag cccgatcgtc
     7441 caataggagc ccgccagcaa tcgcagcgcc gccccgatct tgcgtccgag cgtgtcgtcg
     7501 tcggcgacga cgcgcgccac gtgcgactgc gaagcgggat ccgcgagcag atcccaggct
     7561 gcgaagatcc cggcgagaaa caccgtggcg atcgcgatgc ccagagcggc cgcgcgtgac
     7621 ggacgccgtc cggccgcgag cgcgaggaac aggccgaacg cgggcacggc gacgaagatc
     7681 gagccgaact tggcgccggc cgacggcgcg gccatcgccc ccgccgtgag cagcaggagc
     7741 ggcggagccc acttgagcgc atcgcgccgg gcgtcgagcc attggccggt ggcgagcaag
     7801 gcgccggcga ccgcgacgcc catcatctcg tttccgacac cgaagaaacg gtctccgccc
     7861 gcgatcgaat acgacagcgc ggagcgtgcg ccgagctcgc cctggccgac gaaggcgatc
     7921 agacccgacg ccgcgatccc ggcgcacacg ccggctgcga agggaatgcg ccggacggcg
     7981 ccggccacga gcgcgagccc gagcgaggcc gacgtgatca ccgcaacgga tgcggcaagg
     8041 gtcgtgacgc ccgtgagcgg ctcgatcagc atcgcggccg gtgcggccac gagcgcgacg
     8101 agcagcgtcg cgagccaatc gcgtcccgtt cgcggcagcc ggccgccgcg cgcacggccg
     8161 cggcccgcga gcacgaccac cgctgcgacg acgatcaagc ctgccgccag ccacacgtat
     8221 ccgcgcatca cgggaagcct tatcgacgcg gcatgcgtga agtcccggtc ggcgcgccgc
     8281 agatactccg gcgtgcccgg ctcgctgccc caccgccggc cgacgccgac ggtggcatcg
     8341 ccaccggcga gagcgacgat cgtgcttgcc gcgtcggtga gcgagaccac gccgcgccgc
     8401 cgggtcgtgg gcgaggtaag gatcgccgcg tcgtcgcgga cgggattctc cagcgtggcc
     8461 gcggccagat agttccgccg gtcctcgcgc gcggcggggg gcacggccga catcaccagc
     8521 atccggtcgc cttgctcgag cgaggatcgc acgacgccga tggtggcgtc cgcgcggccc
     8581 aacgcgacgc tcacccaccg gtcgcgctgc cgtccgcccg cgcagtccgc atacagggtg
     8641 tcgggaatgt ccacgatcac gaccccctcg gccgcggttt cgtattggcg ggcaaggcgg
     8701 ctctggtcgg ctcgccggcc ggtcggaaag gacgagtcac gcacgaccgg tgtgtccagc
     8761 agccgcaggg tcgtttcggc gcgacgcagc gcctcgcgga gcggaacggc gccgccggac
     8821 accgacgcgc gcgaccgggt tccgttcatc aacgtcgcca tcgcgtggcc cggccgagca
     8881 aggcactcgc gctcgccggc cggtggcttc gcggcggtcc gggcggaaag caagcccggc
     8941 gttccctcgc ggatcgcgcg gcgcagatga aaggtgtcgg cgcgaagcca gtcctcgagc
     9001 gaggtcccgt tgagcagcac caccacgatt cgcccggttg cgggctcgat ctccggcgcg
     9061 ctggcggcgc gcgcgggaag cagcccggcg gccagcaagg ggaccaggac ggcgagcatt
     9121 ccgagcgcga cgcgtcgcat catgccgcgg cgagtgtatc gggcgtagaa tcgcggctca
     9181 tgactgcatg ggatctggac gacggagttc tgcgcgaggt tctctccgag gccctcgcga
     9241 ccggcgggga cttcgcagag atcttcgccg agaaccgcga ttcgatgtcg gtccggctcg
     9301 aagatcgaaa ggtcgaagag ctcgtggccg gccgcgaccg cggggcgggc atcagggtcg
     9361 taaagggcga gtccaccgcc ttcgccttca ccaaccggct cgaccgcggc gcgctggtag
     9421 atgcggccaa ggccgccgcg gcgggcctga ccgatcagcg gctcgtggac gtgcaggatc
     9481 tgcggcgcga gcccgagacc gtgctcaatc ccgtgcgcat cgatgcgcgc gaggtcccgg
     9541 ccgatcgcaa ggtcgcctac gtcgtcgcgc tcgacgaagc cgcgcgcgcc gagggcggcg
     9601 gcgagatccg tcaagtcatt ggttcctacg tcgaaggctc gcagcgcgtg ctcatcgcca
     9661 actccgacgg cctgatcgtg cgggacaacc gcacccgcac gcgcgtgtcg tgccaggtcg
     9721 tggccgcgcg cggggacgtg atccagaccg gttttcactc gccgggcgcg tcggcgggct
     9781 tcgagcacct cgacgcgcac ccggccgact tcgtcggacg attcgcggcc cggcaggcga
     9841 tcgccatgct cggctcgatc cccgcgccgc cgggcgccag caccaccggc atctcgccgg
     9901 ccggcggcgg cggcgtgctg ttc
//
LOCUS       DATLFO010000090         3720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_102576, whole genome shotgun sequence.
ACCESSION   DATLFO010000090 DATLFO010000000
VERSION     DATLFO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102576"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2106)
                     /locus_tag="VM841_03005"
     CDS             complement(<1..2106)
                     /locus_tag="VM841_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVL89184.1"
                     /translation="MSPRSLLPRAAVIAVFVGLTGTFGAGQVATPPVPIDSDDIGGVV
                     TGPRGPEAGVWVIAETTQLPTRLIRIVVTDDQGRYVLPDLPKATYDVWVRGYGLVDSE
                     KVQATPGRQLPLKATPAPSARVAAEYYPAQYWLSLLQVPPKSDFPGTGPTGNGISPNV
                     RSQGEWIRTIVNTDGCTGCHQMGNKATREIPEMLGRFSSSTAAWERRVQSGQAGAGML
                     ARFTQVGRDRSLAMWADWTDRISAGELPATAPPRPQGRERNVVITMWDWADPKAYLHD
                     EIASDKRNPTVNANGPVYGAMEESADYMPVIDPVRHTASRIQLQVRDPNTQRPGKPAA
                     SSPYWGDEMIWTAQTTAHSFAMDKQARVWIAARIRPNQTPAFCRQGSDHPSAAAFPIN
                     QSGRQMQLYDPTTKTVTTIDTCFGTHHLNFDDNDVLWFTGGGAVEGWFDTRIWDKTKD
                     EQKAQGWTVFVLDTNGNGRRDEYVEPDQPIDPAKDKRVNAPFYGVAPSPVDGSIWGSV
                     LGMPGMLVRLTLGSNPPATALTEVYEVPWNNPNASAQGFAPRGMDVDSNDVVWTVLSS
                     GHLASFDRRKCRGPLNGPTATGQHCSEGWILHALPGPNYKGAEDSASADTAYYNFIDR
                     FDMLGVGKDIPLATGNGSEGLLALVDGRFMTFRVPYPMGFYAKGMDGRIDDARGGWKG
                     KGIWTTYATRAPFHVEGGKG"
     gene            complement(2194..>3720)
                     /locus_tag="VM841_03010"
     CDS             complement(2194..>3720)
                     /locus_tag="VM841_03010"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HVL89185.1"
                     /translation="VSRLKLVWSAPTGEVGAHQAPPLVNNGVMFVSTPNNQVAAYDAK
                     TGMLLWRYKRERPQGAFVAHPSNRGLALYGDKVYYAAGEAVVVALDAKTGREVWAKEV
                     FNNKINYYITLAPLVADGKILVGASGGEFGIRGYIAALDPENGNELWRTYMVPAPGEP
                     GSETWPKDGEQWKTGGASVWVTGNYDPETNTSFWGTGNGGPWMGDQRPGDNLYVASTV
                     ALDASTGKIKGHMQYTPNESFDWDEVSPPILVDFRRGGREVKGAINVARNGYMYFLDR
                     TGGALRFVDGKPYVNQNVFRSLDPKTGRPDVIEENKPGTGKRATYCPSLHGGKNWPPI
                     AYSPRTRMIYVPANNNLCQTMEGAPVTYTPGRGFTGTARGEGLGTNLRAGATHVGEVQ
                     AWNVDTGEKVWTHTYRQANWGPMLATGGGLVFSGGTPDQNFRAFDAANGRLLWEFKTS
                     SGVLGPPASFEVDGKHYIAVLTGWGADASGAGPTVARLLGEPIPSVPSGGAVYVFAVE
                     "
BASE COUNT          558 a         1247 c         1214 g          701 t
ORIGIN      
        1 gcccttgccg ccttccacgt ggaagggtgc gcgcgtcgcg taggtcgtcc agattccttt
       61 gcccttccac ccgccgcgcg cgtcgtctat acgtccatcc atgcccttcg cgtagaagcc
      121 catgggatag gggacgcgga acgtcatgaa cctgccgtcg acgagcgcga gcagtccttc
      181 tgacccgttg ccggtcgcga gcggaatgtc cttgccgacg ccgagcatgt cgaagcgatc
      241 gatgaagttg tagtacgcgg tatctgcgct cgcggaatcc tccgcacctt tgtagttcgg
      301 ccccggcagc gcatggagta tccacccctc cgaacagtgc tggccggtcg ccgtcggacc
      361 gttgaggggg ccgcggcatt tacggcggtc gaagctggcg aggtggccgc tggacagcac
      421 cgtccagacc acgtcgttgc tgtcgacgtc cattccccgc ggcgcaaagc cctgtgctga
      481 cgcgttcggg ttgttccacg ggacctcgta cacctcggtg agcgccgtgg ccggcgggtt
      541 cgagccgagc gtcagccgga cgagcatccc tggcatgcca agtaccgatc cccagatcga
      601 tccgtcaacg gggctcggcg ccacgccgta gaacggcgcg ttcacacgct tgtccttcgc
      661 gggatcgatg ggctggtccg gttcgacgta ttcgtcgcgc ctgccgttgc cgttcgtgtc
      721 gaggacgaac accgtccatc cctgtgcctt ctgctcgtcc ttcgtcttgt cccagatgcg
      781 ggtgtcgaac cacccttcga cggccccgcc gccggtaaac cagagcacgt cgttgtcgtc
      841 gaagttcaga tggtgcgtgc cgaagcaggt gtcgatcgtg gtgaccgtct tcgtggttgg
      901 atcgtagagc tgcatctgcc gcccgctctg gttgatcgga aacgcggcag cagaaggatg
      961 atctgagccc tgtcggcaga acgccggcgt ctggttcggc cggatgcgcg ccgcgatcca
     1021 gacacgagcc tgcttgtcca tcgcgaagct gtgcgcggtc gtctgcgcgg tccagatcat
     1081 ttcgtcgccc cagtaaggcg aggacgccgc cggcttgccg ggacgctgcg tattcgggtc
     1141 gcggacctgc agttggatcc tcgaggcggt gtgccgcacc ggatcgatca ccggcatgta
     1201 gtcggcgctc tcttccatgg cgccgtaaac cggcccgttg gcgttcaccg tcggattgcg
     1261 cttgtcgctc gcaatctcat cgtgaaggta cgccttggga tccgcccagt cccacatcgt
     1321 gatcaccacg ttgcgttcgc gcccctgcgg acgcgggggc gcagtcgcgg gcaactctcc
     1381 ggccgaaatg cgatccgtcc agtcggccca catggccagg gaacgatcgc gtcccacctg
     1441 ggtgaatcgc gccagcatgc ccgcgcccgc ttgccctgac tggacgcgac gctcccatgc
     1501 ggccgtcgac gacgagaacc ggccgagcat ctcggggatc tcacgcgtcg ctttgttgcc
     1561 catctggtgg caacccgtgc agccgtcggt attgacgatg gtgcggatcc attcgccctg
     1621 gctccggacg ttgggtgaga tgccgttgcc ggtgggcccg gtgcccggaa agtcgctctt
     1681 cggcgggacc tggaggagag acaaccaata ctgcgccggg tagtactcgg ctgcgacgcg
     1741 cgcgcttggc gccggagtcg ctttcaacgg gagctgccgg ccaggcgttg cctgcacctt
     1801 ctcggaatcg acgaggccgt agccgcgcac ccagacgtca taggtcgcct tcggcagatc
     1861 cggcagcacg tagcggccct ggtcgtccgt gacaacgatc ctgataagcc gcgtagggag
     1921 ctgcgtcgtt tcggcgatga cccagacgcc ggcctccgga ccgcgcggtc cggtgacgac
     1981 cccaccgatg tcgtcgctgt cgatggggac tggaggcgtg gcgacctgac ccgccccgaa
     2041 ggtgccggtc aggcctacga aaaccgcgat gaccgcggcg cgtgggagga gtgaacgcgg
     2101 gctcatgtgg cctccaactc ccaatctcca attcccaact cccgaacgcc gcgatgcctt
     2161 gggcgttggg agttggggat ggggatttcc gatttattcg actgcgaaga cgtagaccgc
     2221 gccgcccgag ggcacgctgg ggatgggttc acccagcagt cgcgcgaccg tggggccggc
     2281 gccgctggcg tctgcgcccc agcccgtgag cacggcgatg tagtgctttc cgtccacctc
     2341 gaacgacgcc ggcggtccga ggacgccgga gctggtcttg aattcccaca ggagcctgcc
     2401 gttggcggca tcgaacgcgc ggaaattctg atccggcgtg ccgccgctga acacgagacc
     2461 gccgcccgtg gcgagcatgg ggccccagtt cgcctggcga tacgtgtgcg tccagacctt
     2521 ttcgcccgtg tcgacattcc acgcctgcac ttcgccgacg tgggtcgcgc ccgcacgaag
     2581 gttcgtcccg aggccttccc ctcgcgcggt accggtgaac ccgcgtcccg gggtatacgt
     2641 cacgggcgca ccctccatgg tctggcacag gttgttgttc gccgggacgt aaatcatcct
     2701 cgtgcgaggg ctgtacgcaa ttggcggcca gttcttcccg ccgtgaagcg aagggcagta
     2761 ggtcgctcgc ttaccggtgc ctggcttgtt ttcctcgatc acgtcgggac ggcccgtttt
     2821 cggatcgagc gaccggaaga cgttctgatt cacgtacggc ttgccgtcga caaaccgcag
     2881 tgcgccgccc gtgcggtcga ggaaatacat gtagccgtta cgcgccacgt tgatggcgcc
     2941 cttgacctcg cggccgccgc gtcggaagtc gacgaggatg ggcggcgaca cctcatccca
     3001 gtcgaacgac tcgttcggcg tgtactgcat gtggcccttg atctttccgg tcgaggcatc
     3061 gagcgccacg gttgacgcga catacaggtt gtcgccgggc cgctggtcgc ccatccacgg
     3121 gccgccgttg ccggtgcccc agaacgacgt gttggtttcg gggtcatagt tgccggtgac
     3181 ccacacggag gcgccgcccg ttttccactg ctcgccatcc ttcggccagg tctcgctgcc
     3241 gggttctccg ggcgccggca ccatgtaggt gcgccacagc tcgttaccgt tttcaggatc
     3301 gagtgccgcg atgtacccgc ggatgccgaa ctcgccgccc gacgcgccaa cgaggatctt
     3361 gccgtcggcg acgagcggag ccagcgtgat gtagtagttg atcttgttgt tgaagacttc
     3421 cttcgcccac acttcgcggc cggtcttcgc atccagcgcc acgaccacag cctcgccggc
     3481 ggcgtagtac accttgtcgc cgtagagggc gagcccgcgg ttggacggat gggcgacgaa
     3541 cgccccctgc ggccgctccc gcttgtagcg ccagaggagc atgccggtct tcgcgtcgta
     3601 cgccgcgacc tggttgttcg gcgtgctcac gaacatgacg ccgttgttca ccagcggcgg
     3661 cgcctggtgc gcgccgactt caccggtcgg cgccgaccac accagcttca gccgcgacac
//
LOCUS       DATLFO010000091         5181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_52413, whole genome shotgun sequence.
ACCESSION   DATLFO010000091 DATLFO010000000
VERSION     DATLFO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52413"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VM841_03015"
     CDS             complement(<1..1819)
                     /locus_tag="VM841_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVL89186.1"
                     /translation="MPLTADRRAGVWQPVKRLVVGRRLATEEAPHQLLPKIVALPIFA
                     SDPLSSNAYATEEILIVLSAAGASAFWLATPLSWMIAAVVIVVITSYRQTIKAYPRGG
                     GSYVVAKENLGTNYGLVAAASLLADYILTVAVSVSAGVAAITSAAPGLADKRLSLAIG
                     FVALLMIANLRGLKESGALFAVPTYAFVLSIWSLLMVGGVKCIGGCPQAASAAFEPEV
                     TSSLSLLLVLRAFASGSTALTGIEAVADGVPAFRGRRPSEQARNAIITITVMGFFTLS
                     MFLGITLLARGTNVHPITEEIAHRFGIHEATIVSQIAEALSGRGLLFFAVQATTAAIL
                     ILAANTAYADFPRLSSIIARDRFLPRQFMNRGDRLVFSNGIIALSLLAVMLIWLFDAS
                     VTKLIQLYVVGVFTTFTLSQAGMVRRWRRLRTPGWRIKAAVNTVGAATTGLVLVVVAI
                     TKFRHGAWIVITAIPIIVVGFKAVARHYGDIAGQLRLAGSRPEQPVATHVIIPVARVD
                     EATLRAVGYAKMIRPLSLRAVHVAEAGDPEATELADMWRQWSMGCPLEIVERDDMIGG
                     VRDAIRSLRPREGEMLTVMIPERLRGRGWLQFMSRRRGLL"
     gene            complement(1803..1976)
                     /locus_tag="VM841_03020"
     CDS             complement(1803..1976)
                     /locus_tag="VM841_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89187.1"
                     /translation="MIVFLAGLLVGAMLSAIGLPRGALLWRAVRADDAEMLHNAEAAR
                     VISLNGTAGAPHR"
     gene            2111..3502
                     /locus_tag="VM841_03025"
     CDS             2111..3502
                     /locus_tag="VM841_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89188.1"
                     /translation="MRRLQPAARGLLVALATTGTATLLAFGGEVVPTSVAALAFVLAV
                     TISAAVGGLWAGIAASLISFPALNFFFTAPRFTFAVEKTEDVVALFVLLAVSGIVGTL
                     LSSVVRQRARAERREREARLLHSVATRLLSGEPVDGVLRRFAEAITDLFALARCEVQS
                     PLVAAPVVVDRGDGAAPQDEFPMTTAAGEAGRILVRAKGILSEDDRDVLRTFAGQIAL
                     ALERLRLAGEAQSAQLDAEESRLRAALFSSVTHDLRTPLASITAAVSSLQDEQTTFSD
                     EDRRDLLDTIRGEAERLNRLVGNLLNLARMRAGALKPDKKPAGIDELIEAVLARLRRL
                     LAGRDVRLKLREDLSEIPIDVLQIDQVLTNVIENACKYSPPGSEITVTAARWHDDVEV
                     RVADRGSGIPESERTRVFEPFVRRDDDPRAGSGLGLSIAQAIVVAHGGAMWIEPTPGG
                     GTTVAFRLPGRGR"
     gene            3499..4185
                     /locus_tag="VM841_03030"
     CDS             3499..4185
                     /locus_tag="VM841_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017691168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89189.1"
                     /translation="MTVSRVLVVDDDPQIRRAITTSLRARRFEVAAAADGEEALTKLA
                     AGEFDCVILDLGLPGIDGHEVIRRLREWSPIPVIVLSVRDSQHDKIDALDAGADDYVT
                     KPFAMDELLARMRAIERRAATDKPPPVLHFGELEIDLARQLVRLGGAAVPLTPTEYRL
                     LTAMASHPGKLLTHQWLLGKVWGPGYTSESLRVYVKQLRHKLGEDPVRPRWITTEPGM
                     GYRWSADPAE"
     gene            complement(4186..>5181)
                     /locus_tag="VM841_03035"
     CDS             complement(4186..>5181)
                     /locus_tag="VM841_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89190.1"
                     /translation="VLVVVTASRFVQGAWIMMVAIPLIALSFAAVNKHYREISVQLRK
                     PDGRPERVTNTHVLIPVARVDEATLRAVGYAKMIRPLSLRAVHVASPDDPEAAELAAL
                     WNEWAMGCPLEIVPRSEMIRGIRDAIRAIEAAPGEMRTVVVPEQLRGRGWLQFLRRRR
                     GLLLKATLLFERNLVVTDVPVVESQHRRPIGTRPPAPVRNEVVVLVSAVHNASLSAVA
                     YAAGLRPTGIRAVTFAVDEEDTEKLMEDWTRQPFDVPLEILDSPYREVRKPLIRLVRE
                     LKAEAPGTVVTVVLPEFVVRKRWHQFLHNQTALSIKAGLLFEQNTVVTSVPFHLE"
BASE COUNT          830 a         1727 c         1836 g          788 t
ORIGIN      
        1 gcagcaaacc ccgccggcgg ctcatgaact gcagccaccc gcggccgcgg aggcgctcag
       61 ggatcatcac ggtcagcatc tcgccttcgc gcggacgcag cgaacggatc gcgtcgcgca
      121 cgccgccgat catgtcgtcg cgctcgacga tctccagcgg gcagcccatg gaccactgcc
      181 gccacatgtc ggccagctcg gtggcttccg ggtcgcccgc ttcggcgacg tgcacggccc
      241 gaagcgagag cggccggatc atcttggcgt agccgaccgc gcgcaaggtg gcttcgtcca
      301 cgcgggcgac cgggatgatc acgtgggttg cgaccggctg ctcgggccgg cttccggcca
      361 gacgcagctg gcccgcaatg tcgccgtagt ggcgcgcgac agccttgaag cccaccacga
      421 tgatcgggat ggcggtgatg acgatccacg caccgtgccg gaacttggtg atcgcgacca
      481 ccaccagaac caggccggtt gttgccgccc cgacggtgtt gacggccgct ttgattcgcc
      541 agccgggcgt gcgcagccgg cgccaccggc ggaccatgcc ggcctgcgac agcgtgaagg
      601 tcgtgaagac tccgacgacg tacagctgga tgagcttggt gacgctggcg tcgaagagcc
      661 agatcaacat caccgccagc aaagacaacg cgatgatgcc gttggaaaac acgagccggt
      721 cgccgcggtt catgaactgc cgcggcagga aacggtcacg cgcgatgatc gacgacaggc
      781 gggggaagtc tgcgtaggcg gtgttggcgg ccagaatcag aatcgcggcc gtcgtcgcct
      841 ggaccgcgaa gaacagcaat ccgcgtcccg acagcgcctc ggcgatctgc gacacgatcg
      901 tcgcctcgtg gatgccgaac cggtgggcga tctcttcggt gatcgggtgc acgttcgtcc
      961 cgcgggccag cagcgtgatg ccgaggaaca tcgacagcgt gaagaagccc atcaccgtga
     1021 tcgtgatgat cgcgttgcgc gcctgctcgg acgggcggcg gcctcggaag gccggcacgc
     1081 cgtcggcgac ggcctcgatg ccggtcagcg ccgtcgatcc ggatgcgaac gcccgcagga
     1141 ccagcagcag cgacagcgac gacgtcacct cgggctcgaa ggcggccgag gcggcctgcg
     1201 ggcagccgcc gatgcacttg acgcccccca ccatcagcag cgaccagatc gacagcacga
     1261 acgcgtaggt gggcaccgcg aacagcgcgc ccgactcttt caggccgcgc aggttcgcga
     1321 tcatcagcag cgcgacgaac ccgatcgcca gcgacaagcg cttgtcggcc aggcctggtg
     1381 ccgccgaggt gatcgccgcg acaccggccg agacgctgac cgcgacggtc aggatgtagt
     1441 cggccagcag cgacgcggcc gcgaccagcc cgtagttggt gccgaggttc tccttcgcga
     1501 ccacatacga cccgccgccg cgcggatagg ccttgatggt ctgacgatac gaggtgatca
     1561 ccacgatcac gacggcagcg atcatccacg acagcggggt ggcaagccag aatgcgctcg
     1621 cgccggcggc cgacaggacg atcaggatct cttcggtcgc gtaggcgttg gacgaaagcg
     1681 gatccgaggc gaagatcggc agcgcgacga tcttcggcag caactgatgc ggcgcctctt
     1741 cggtcgcgag ccggcgaccc acgaccagcc gcttgaccgg ctgccagacg ccggcacggc
     1801 ggtcagcggt gaggggcacc ggcggtcccg ttcagggaga tcacgcgcgc agcctcggca
     1861 ttgtggagca tttcggcgtc gtcggcgcga acggcccgcc aaagcagagc cccgcggggc
     1921 agcccgatcg ccgaaagcat cgctccgacc agcagcccgg cgaggaacac gatcatgcct
     1981 cgatgatcga tccccggccg tgagccgccc gtgaggaatc cgccgccggc cgtgagaatg
     2041 ttgtgaggat cggctccacg gcggtggcga agcgagcctg cgggtggtgt gatctgcggg
     2101 tgtgaacggg atgcgccggc ttcaaccggc cgcgcgaggc ctgctcgtgg cgctggcgac
     2161 gaccggtacc gccacgctgc tggcattcgg cggcgaggtg gtgccgacct cggtcgcggc
     2221 tttggcgttc gtgcttgccg tgacgatcag cgcggcggtg ggcggcttgt gggcggggat
     2281 cgccgcatcg ctgatctcgt ttccggcgct gaatttcttc ttcaccgcgc cgcggttcac
     2341 gttcgccgtc gaaaaaaccg aggacgtcgt cgccctgttc gtgctgcttg cggtatccgg
     2401 aatcgtcggg acgttgcttt cgtcggttgt gcgccagcgt gcacgggccg agcggcgcga
     2461 gcgcgaggcg aggctgctgc attcggtcgc gacccgcctg ctgtcgggcg aaccggtcga
     2521 tggcgtcctt cgccggttcg ccgaagcgat caccgatctg ttcgcgctgg cccggtgcga
     2581 ggtgcagtcg cctctcgtgg ccgctccggt cgtggtggat cgcggcgacg gcgctgcgcc
     2641 gcaggacgag tttccgatga ccactgcggc gggcgaggcg ggacgcatcc tggttcgggc
     2701 caagggaatc ctgagcgagg acgaccgcga cgttctgcgt accttcgcag gccagatcgc
     2761 gctggccttg gagcgccttc ggctcgccgg cgaggcgcag tcggcgcagc tggacgcaga
     2821 ggaaagccgg ctgcgggccg cgttgttctc ctcggtcacc cacgatctgc gcacgccgct
     2881 tgcttcgatc accgccgcgg tgtcgagcct tcaggacgag cagacgacgt tctccgacga
     2941 ggaccgccgg gatctgctcg acaccattcg cggcgaagcg gagcgtctca accgtttggt
     3001 cggcaatctg ctgaacctgg cccgcatgcg cgccggggcg ctcaagcccg acaagaaacc
     3061 ggccgggatc gacgagttga tcgaagcggt gctggcgcgg cttcgccggt tgctggcagg
     3121 ccgtgacgtg cggctgaagc tgcgcgagga tctgagcgag atccccatcg acgtgcttca
     3181 gatcgaccag gtgctgacca acgtgatcga aaacgcgtgc aagtacagcc ctcccggctc
     3241 cgagatcacc gtcactgccg cgcgatggca cgacgacgtc gaagtgcgcg tcgccgaccg
     3301 cggctcgggc atccctgaat ccgaacgcac ccgcgtgttc gagcctttcg tgcgccgcga
     3361 cgacgatccg cgcgccggat ccgggctggg cctgtcgatc gcgcaggcaa tcgtggtcgc
     3421 acacggcggc gcgatgtgga tcgagcccac gcccggcggc ggcaccacgg ttgcgttccg
     3481 tttgccgggg cggggacgat gacggtatcg cgggtgctgg tcgtcgacga cgatccgcag
     3541 atccgccgcg cgatcacgac cagccttcgc gcacgacgtt tcgaggtggc cgccgcggcc
     3601 gacggcgaag aagcgctcac caagctcgcc gccggcgagt tcgactgcgt gattctggat
     3661 ctcggcctgc cgggcatcga cggccacgag gtgatccgcc ggctgcgcga gtggtcgccg
     3721 attccggtga tcgtgctgtc ggtgcgcgac agccagcacg acaagatcga cgcgctggat
     3781 gccggcgcgg acgactacgt taccaagccg ttcgcgatgg acgagctcct ggcgcgcatg
     3841 cgcgcgatcg aacgccgcgc ggcgaccgat aagccgccgc cggtgctgca cttcggcgag
     3901 ctcgagatcg atctggcgcg ccagttggtg cgtctcggcg gcgctgcggt cccgttgacg
     3961 ccgaccgagt acaggctgct gaccgcgatg gcgtcgcatc ccggcaagct cttgacgcac
     4021 cagtggctgc tcggcaaggt ctgggggccc ggctacacca gcgaatcgtt gcgtgtctac
     4081 gtcaagcagc ttcggcacaa gctgggtgag gatccggtgc ggccgcgatg gatcacgacc
     4141 gagccgggca tgggctaccg ctggagcgcc gatccggccg agtgactact ccagatggaa
     4201 cgggacgctg gtgacgaccg tgttctgctc gaacagcagg ccggccttga tcgacagggc
     4261 ggtctggttg tgcaggaact ggtgccagcg tttgcggacg acgaattcgg gcagcacgac
     4321 ggtcacgacg gttccggggg cttcggcttt gagttcgcgg accagtcgga tgagcggctt
     4381 gcggacttcg cggtaggggg agtccaggat ttcgagcggc acgtcgaagg gctggcgggt
     4441 ccagtcttcc atcagctttt cggtgtcctc ttcgtccacg gcgaacgtga ccgcgcggat
     4501 gccggtcggg cgcaggcccg ctgcgtaggc gacggcgctc agcgacgcgt tgtgtacggc
     4561 cgagaccagc acgaccacct cgttgcggac cggcgcgggc ggtcgcgtcc cgatcggccg
     4621 gcgatgctgg gactcgacca ccggcacgtc cgtgacgacc aggttgcgct cgaacagcag
     4681 cgtcgccttc agcagcaggc cgcggcgccg ccgcaggaac tgcagccagc cgcggccgcg
     4741 cagctgctcg ggcaccacaa ccgtgcgcat ctcgccgggg gcggcctcga tcgcgcggat
     4801 cgcatcgcgg atgccgcgga tcatctcgct tcgcgggacg atctccagcg ggcagcccat
     4861 cgcccattcg ttccacagag cggccagctc ggccgcctcc gggtcgtcgg gcgaggcgac
     4921 gtgcaccgcc cgcagcgaga ggggccggat catcttggcg tagccgaccg cgcggagcgt
     4981 cgcttcgtcc acccgcgcga ccgggatcag cacgtgcgtg ttggtgacgc gctcgggccg
     5041 gccgtccggc ttgcgaagct gaaccgagat ctcgcggtag tgcttgttga cggccgcgaa
     5101 cgacagcgcg atcagcggga tcgcgaccat catgatccag gcgccctgca cgaagcggct
     5161 cgcggtcacg accaccagga c
//
LOCUS       DATLFO010000092         5122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_52428, whole genome shotgun sequence.
ACCESSION   DATLFO010000092 DATLFO010000000
VERSION     DATLFO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52428"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VM841_03040"
     CDS             <1..283
                     /locus_tag="VM841_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVL89191.1"
                     /translation="VPTPRSGHGSGFMKGRVIVTGGEDPAIGSGQPSISSTEAYDPLK
                     GVWFKDMPGMFRARHGVGTVVIDEMLHVFLGGTRVGLAPSADADVFIIP"
     gene            280..960
                     /locus_tag="VM841_03045"
     CDS             280..960
                     /locus_tag="VM841_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018727117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVL89192.1"
                     /translation="MTRDSLRAVAAEIESCFACPRLVAWREQAARDKPARYADDEYWA
                     RPVAGFGDAKARLLIVGLAPAAHGGNRTGRVFTGDRSGDWLFRALHKAGYANRPASVS
                     RDDGLKLTDAYVAAVVRCAPPANKPTTGERDTCIPYLEREVRLLREMRVAVALGSFGW
                     DGFLRVARVLGHAPARKPRFGHLAEAAVGPWTLLGSYHPSQQNTFTGTLTEEMFDAVF
                     ARARELGG"
     gene            1180..3204
                     /locus_tag="VM841_03050"
     CDS             1180..3204
                     /locus_tag="VM841_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89193.1"
                     /translation="MRKTRALLAVVSLAASLAAPARPARAAAFEIGRLVAAKPAAVAV
                     DPSIHAMFVAGQDGGLWIYDTRTYASRTVELTCCLVAAVADAATHRVYVSGNGLIVLD
                     GVAGVVRATVSLAPGTPEALALDSAGGRLYVADPPGDRVVVLDTATDTIVSSIPVRDR
                     PAGLAIDPAAGVLYVASGGEEMVDAIDVSTGATIASAPVAAPSGIATGNGEIYVASGE
                     GAVVALRAGTLAAVRSIATGAMPRSVAYNPVSNRLWVANWGSGTVSFVDPVTGTVLGT
                     MPSGTRPSHVAFDAGLGRTYVAVAGSDEVQLLHERAATISIATPGEGQIVGPAKRTIA
                     GSAPPGAPVTIFEGAAPAGSGFAGPTGAFSIQVSLGEGTRTVVAAVPYEPYSAPRTFT
                     VDLTTPVIEHAGRTPAGPSGWNNTPVRLTWTCTDALSGVKSEQIEKLLGFDGRGQSAT
                     ATCEDRAGNTASHTVTGIDIDLRGPVISMSRSPAATAGWNRTSPVTVAFTCADTMSGM
                     PSPSSRSVTVSGETDGTEVAHQCADVAGNVSSRVEVVRIDSIAPVASVDQTVPAMTRL
                     PVEIDGTLRSGITGSVTDWASGPVAADVRFSDESGLERVVAASCASGCGSGAIRWRVR
                     PPPSMNPGWYEVSARGTDLAGNVGGWSAPARIFIATGEEWPRIPLPNLPI"
     gene            3421..4293
                     /locus_tag="VM841_03055"
     CDS             3421..4293
                     /locus_tag="VM841_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89194.1"
                     /translation="MLRRRDPSRRPGGRGSFAAWMLVTVGLLVPSQWFAYRSAMGAGG
                     DERFPISAWRDPKYFSSEAQTRTVQPLLRRIIDEIAATEPAAGAGPRPGGGPTNQPSP
                     SPSPSGGKTPLPSASPTPSPTVSASPTPRPSRPSIEIRADHIPAPDGSDSLFYRWRIQ
                     IENPADGTWLDDLETLTQVPPGMQRVECGRGVSVDAGSPGGLTSAAAPGITCHAVPPP
                     ASEDGHEMRWQESQLGPGWVRVYTFEVRVTAPNGTAIRNHAHLQWRGGSRSTGLCELT
                     VNRQSPKGCVAGPG"
     gene            4471..>5122
                     /locus_tag="VM841_03060"
     CDS             4471..>5122
                     /locus_tag="VM841_03060"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HVL89195.1"
                     /translation="MRRPRYRDLPIAAKLLVPFLISMIVWGVLGTQVLLSGSAREASG
                     VASTRLAVALEVGRSAFSTASARLIETVRFAAHTEGLGDALDRRDSVRLTQLIEGIAA
                     NAGHERLQVIDRGGTVVVTVKLAGTEPVAATGGRATHPLIVDAARGVITAESTVVLDG
                     GRVMAVVPAKNLAGEVVGAVVAAETAERIVGDMAAGTGVRMTLFDASGERVAGAGPP"
BASE COUNT          683 a         1859 c         1870 g          710 t
ORIGIN      
        1 cgtgcccacg ccgcgcagcg ggcacggcag cggcttcatg aagggccggg tgatcgtgac
       61 ggggggggag gatcccgcga tcggatcggg gcagccgtcg atctcctcga ccgaggccta
      121 cgatccgctc aaaggcgtgt ggttcaagga catgcccggc atgttccgcg cgcgccacgg
      181 cgtcggcacg gtcgttatcg acgagatgct tcacgtgttt ctcggcggca cgcgggtcgg
      241 cctcgctccg tcggcggacg cggacgtgtt catcatcccg tgacccgcga ctcgctcagg
      301 gccgtggcgg ccgagatcga atcgtgcttc gcctgtccgc ggctggtcgc gtggcgcgag
      361 caggccgcgc gcgacaagcc ggcgcgctac gccgatgacg agtattgggc gcgtcccgtc
      421 gccggcttcg gcgacgcgaa ggcgcggctg ctgatcgtgg ggctcgcgcc cgcggcgcac
      481 ggcggcaacc ggaccgggcg cgtgttcacc ggcgaccgca gcggcgattg gctgttccgc
      541 gcgctgcaca aggccgggta tgcgaaccgg cccgcatccg tctcgcgcga cgacgggttg
      601 aagctgaccg atgcctacgt cgccgcggtg gtgcgctgcg cgccgccggc gaacaagccc
      661 acgaccggcg agcgcgatac ctgcatcccg tatctggagc gcgaagtgcg gctgctgcgc
      721 gagatgcgtg tggcggtcgc gctcggctcg ttcggctggg acggcttcct gcgcgtcgcg
      781 cgcgtgctgg gccatgcgcc ggcccgcaag ccgcggttcg ggcacctggc cgaagcggcc
      841 gtggggccct ggacgctgct cggctcctac cacccgagcc agcagaacac cttcaccggc
      901 accttgaccg aggagatgtt cgacgcggtc ttcgcccgcg cgcgcgagct gggcggctag
      961 cacgaacgag gccgcaaccg ggttgcggct gcggcctcgt ggggggacac aggagggcgg
     1021 tgcggtcttc tgattggaag acaccgccga ggagcggttc tttcacgtgg ccgcgatgtt
     1081 cgtttcgccg cggcaaatcc gccgtggcgg ccccgcaaaa cggccgaacg gaaacgcgcg
     1141 ggcgaggatg gcggcggccc ggcggcgaag tcatgccgga tgcgcaaaac ccgggcgctc
     1201 ctcgcggtgg tatcgctggc ggcctcgctg gcggctccgg cgcggccggc ccgtgccgcc
     1261 gcgttcgaga tcggccggct ggtcgcggcc aagcctgccg ccgtcgcggt cgacccgtcg
     1321 atccacgcga tgttcgtggc cgggcaggac ggcggcttgt ggatctacga cacccgcacc
     1381 tacgcgtccc gcacggtcga gctgacctgc tgcctggtcg ccgcggtcgc cgacgcggcc
     1441 acgcaccgcg tctacgtgtc gggcaacggg ctgatcgtgc tcgacggcgt cgccggggtg
     1501 gtgcgcgcca ccgtctcgct cgcgcccggc acgcccgaag cgctcgcgct ggactccgcg
     1561 ggcggccggc tctacgtcgc cgacccgccc ggcgatcgcg tcgtcgtgct cgacacggcc
     1621 accgacacga tcgtgtcctc gatcccggtg cgcgaccggc ccgcgggcct ggcgatcgat
     1681 ccggccgcgg gcgtgctgta cgtggcgagc gggggagagg agatggtgga cgcgatcgac
     1741 gtgtccacgg gcgcgacgat cgcgtcggct cccgttgcgg cgccctcggg catcgcgacg
     1801 ggcaacggcg agatctacgt cgcgtcgggc gagggtgcgg tcgtggccct gcgcgccggc
     1861 acgctcgcgg ccgtgcgcag catcgcgacc ggcgcgatgc cgcgatcggt cgcctacaac
     1921 cccgtcagca accgcctgtg ggtcgcgaac tggggctcgg gcacggtctc gttcgtcgat
     1981 cccgtcaccg gcaccgtgct gggcacgatg ccgtcgggca ccaggccctc gcacgtggcg
     2041 ttcgacgccg gcctcggccg cacctatgtc gcggtcgccg gatccgacga ggtgcaactg
     2101 ctgcacgagc gggccgcgac gatctcgatc gccacgccgg gcgagggcca aatcgtggga
     2161 ccggccaaac gcacgatcgc cggcagcgcg ccccccggcg cgcctgtgac gatcttcgaa
     2221 ggcgcggcgc cggccggcag cggattcgcc ggaccgaccg gcgcgttctc gatccaggtc
     2281 tccctcggcg agggcacccg cacggtggtc gcagcggtcc cgtacgagcc gtactcggcg
     2341 ccgcgcacgt tcacggttga cctgaccacg ccggtgatcg agcatgccgg gcgcacgccc
     2401 gccggcccgt cgggatggaa caacacgccg gtgcgcctga cgtggacctg caccgatgcg
     2461 ctctccggtg tcaaaagcga gcagatcgag aagctcctcg gcttcgacgg acgcggccag
     2521 tcggccaccg cgacgtgcga ggaccgggcc ggcaacaccg cgagccatac cgtgaccggc
     2581 atcgacatcg acctgcgcgg cccggtgatc tcgatgtcgc gctcgcccgc ggcgacggcg
     2641 ggatggaacc gtacgagccc ggtcacggtc gcgttcacgt gcgccgacac catgtcgggg
     2701 atgccctccc cgtcgagccg cagcgtcacg gtctcgggcg agaccgacgg aaccgaggtc
     2761 gcgcaccaat gcgccgacgt ggcgggcaac gtctccagcc gcgtcgaggt cgtgcgcatc
     2821 gactcgatcg ctccggtcgc gtccgtcgat cagaccgtgc ccgcgatgac gcggctgccc
     2881 gtcgagatcg acggcaccct gcgcagcggg atcaccggct cggtgaccga ctgggccagc
     2941 ggcccggtcg cggccgacgt ccgcttcagc gacgagtcgg gcctcgagcg cgtcgtcgcg
     3001 gcctcgtgcg cttcgggctg cgggagcgga gcgatccgct ggcgcgtgcg cccgccgccg
     3061 tcgatgaacc cgggatggta cgaggtgtcg gcgcgcggga ccgaccttgc cggcaacgtc
     3121 ggcggctggt ccgcgccggc ccggatcttc atcgccaccg gcgaggagtg gccgcggatc
     3181 ccgctgccca accttcccat ctgaccgggc atccggccgg gcatccggct gggcatccgg
     3241 ccgggcatcc gcctgggcat ccggccgtcc ggccgcgcca cccgtgtcgc cccggccgtg
     3301 cgtttccccc gattccggca tccgatccgc acggttcgtc ccggtgaagt tcaagcattc
     3361 cggaatctcc tccgacacta ggaccgaacc cgacgatcaa gcgggagttc tcgcacgagc
     3421 gtgcttcgcc gaagggatcc gtcgcgacgg cccggcggcc gcggaagctt cgcggcctgg
     3481 atgctggtga ccgtcggctt gctcgtgccc tcgcaatggt tcgcgtaccg aagcgcgatg
     3541 ggagcgggcg gcgacgagcg attcccgatc tcggcgtggc gggacccgaa gtacttctcg
     3601 tccgaggcgc agacccgcac ggtccagccg ctgctgcgcc gcatcatcga cgagatcgcc
     3661 gcgaccgagc ccgccgcggg cgccgggccc cggcccggcg ggggaccgac gaaccagccg
     3721 tccccgtccc cgtccccgtc cggaggcaag acgccgctcc cgtccgcgtc cccgacgcca
     3781 tcgccgacgg tctctgccag cccgacgccc cgcccgtcgc gtccgtcgat cgagatccgc
     3841 gccgatcaca tccccgcgcc cgacggcagc gacagcctct tctaccgctg gcgcatccag
     3901 atcgagaacc cggccgacgg gacctggctc gacgacctcg agacgctcac gcaggttccc
     3961 cccggcatgc agcgcgtgga atgcggccgg ggtgtctcgg tcgacgccgg gtcgcccggc
     4021 ggcttgacca gcgcggccgc gccgggcatc acctgccacg cggttccgcc ccccgccagc
     4081 gaggacggcc acgagatgcg ctggcaagag tctcagctgg gacccggctg ggtgcgggtc
     4141 tacacattcg aggtgcgcgt cacggcaccg aacgggactg cgatccgcaa ccacgcgcat
     4201 ctgcagtggc gcggcggctc gcgctctacg gggctgtgcg agctcaccgt gaaccgtcaa
     4261 tccccgaagg gctgcgtcgc gggcccgggt tagcggatca accggcgccg gtgccggaca
     4321 ccgcctgcgg cgggaaaacg cgcgatgcgg ctcatccgga tgcgcgcaag gctggcgatt
     4381 cgggcctatg ccgtaaaggg ggtcagccat ccgttaagcc gtgctcgctg ggggccgaat
     4441 gtatagggaa gccttcggat gaggagctcg atgaggcgac cccgttaccg cgacctgccg
     4501 atcgcggcca agctgctcgt gccctttctg atctcgatga tcgtgtgggg cgtgctcggc
     4561 acgcaggtcc tcctgtcggg cagcgcccgc gaagccagcg gcgtcgcgtc cacgcggctc
     4621 gcggtcgcgc tcgaggtcgg ccgaagcgcg ttctccaccg cgagcgcccg gctgatcgag
     4681 accgtccggt tcgccgcgca caccgaaggg ctcggcgacg cgctggatcg ccgcgacagc
     4741 gtccggctca cgcagctgat cgaaggcatc gccgccaacg ccgggcacga gcggctgcag
     4801 gtgatcgatc gcggcggcac ggtcgtggtg acggtcaagc tcgccggcac cgagccggtc
     4861 gcggccacgg gcggccgcgc gacgcatccg ctgatcgtgg acgcggcgcg cggcgtgatc
     4921 acggccgagt ccaccgtcgt gctcgacggc ggccgcgtga tggcggtcgt gcccgctaag
     4981 aacctcgcgg gcgaagtcgt cggcgccgtc gtggcggccg agacggcgga gcggatcgtc
     5041 ggcgacatgg ccgcgggcac cggcgtgcgc atgacgctgt tcgacgcgag cggcgagcgg
     5101 gtcgcgggcg cgggccctcc gg
//
LOCUS       DATLFO010000093         5750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_43586, whole genome shotgun sequence.
ACCESSION   DATLFO010000093 DATLFO010000000
VERSION     DATLFO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43586"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3403)
                     /locus_tag="VM841_03065"
     CDS             complement(<1..3403)
                     /locus_tag="VM841_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVL89196.1"
                     /translation="MTQGIRPPRRTRGGLRLAIAAAILIASIPAAPAALPTPKRASHG
                     DAAAGHEAAEGYTLAAQERETGAGGPCPDGSPRREVSLTAIAVTVTLNRFGDHDPDGR
                     MFAFTDAIAAIRVEEKRNADARAGSGDYAVTPGLQGDAIQPPVIRVRPGECLRLTLRN
                     ELDEPASLHVHGGALTTGGAPAVATNPSATAAVGATVRYEWAIPSNEPEGTHYFHSHG
                     NERAQTSHGLFGAVVVEPPGATWTDPRTGQGARGWDAVVTHPDGAAFRELTLFYHEIG
                     DEQYQLRAADGSLIPLVDPILSAYRPGSRAINFRSEPFMNRMRLQQQLTGRFDESLAY
                     SSYAFGDPATPVMRAYLGDPVKQRLVHGGSEVFHVHHVHGGSVRWRRQPRAEDSRFDS
                     GLDKRPPLTPEASERTDSQSLGPSESFDIESECGAGGCQQSAGDFLVHCHVAHHYFAG
                     MWGIWRVYNTLQDGAASTDALPPLQELPGRDGHMRAGVPSPALHGSTVTWHGERRTVT
                     DAAGWAAMQLPPAGVPRGYDASVWDWRVEGGVVVGEPEDARSWPGHTPSDPGMRKPIL
                     FDPVSGRLAYPFLRSQFGRRPPFAPNHGPSPYLDPGSGPDPPAPGASGPESLCPEGTE
                     VQTFLINAVSVAVPLNRRAGLSDPNGQIYVLAEDAAAVRADPAKRSPLAIRANAGEDC
                     VDIVLASSLPDGPINHGFSKVNLHVHFVQFDVQGSDGVVNGFNYEQSVRPYDADPVRA
                     RAAAAGAATVAVDDAGRFQRGAVVGIGVGTTRFEARRIERIDGSRLVLDRPLAQSHPA
                     GEIVSAEFVRYRWYPDVQFGTAYFHDHVNALASWRHGLFGALIAEPPGSTYHDPETGE
                     PIRSGAIADIVTKARVSADVTGSFREAVLFLQDDQPIGHEGRSSGSAINQRVEPLRSR
                     EPPPRAFASTRGDPETPLVRARLGDPVVLRTLVAGTNDVHALHVDGHWFRTEAWSRSS
                     RPVSSVRLGISERFDLVIPAAGGPQRMPGDYVYTNGRAFKIREGSWGLLRVLGPGEDS
                     IRALPGREAVTPALRVCPQDAPVRQFRAAAVAATLPALGDVEAATFVHEGDRGGADAP
                     APFVLRAAVGDCIRIALRNALDEPVSLHADMLAYDPATDGGIAAGR"
     gene            complement(3408..4574)
                     /locus_tag="VM841_03070"
     CDS             complement(3408..4574)
                     /locus_tag="VM841_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVL89197.1"
                     /translation="MGWRNATIVAVALTLVGAACSTDKPAGSPDGDGKKSATYTVQVD
                     SHKPASEFGTYFSKFFPGEVKVRAGDSIDFKLNWSGEPHSVTFGTLVDAGAEKLDPTS
                     REEPAEWQKIPLMLPEGPGDAVQAGINPCYVETGDPPKEDLCENRTQPKTFTGTYSMH
                     SSGFLPEGSTYRVTFDSAIDAGTYSYFCTLHRGVMVGKVTVVAATETVPSPDEVATQA
                     DTEIDAIRTKLAGAYDKSKQGMGPGSILAGTGDPTLIEGGTDAGVTEFLPAKAIAKVG
                     ETVKFLPVGPHTVSFNTPQDAVESLVKAGDGTWHINPKAFGPTGSPPSGPPGEKPVVT
                     DAGTWNGEGFLNSGILLGFGPPGALTWQVKFGKAGTYEFRCLLHPDMEGSITVA"
     gene            4758..5525
                     /locus_tag="VM841_03075"
     CDS             4758..5525
                     /locus_tag="VM841_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL89198.1"
                     /translation="MGVHDAAATGFESASGEYERARPGYPADAVDALIRRCEIGPGSR
                     VLDLACGTGKLTRALSPSGARVVGADPVDGMLRRLVESSGGVPVAEATAEHLPFASSV
                     FDAVTVAQAFHWFDGDAALGEIHRVLRPAGGLALLWNIRDTTVPWVGRLNDVFNRYEG
                     DVMRFWRGEWRHAFERSALFTPLERHEFRLDQTLTREGVIDRVLSVSFIATLPAGERA
                     RVIDDVGRILDENPETRGSDRIVLPYLTHLYTCRRTP"
     gene            5522..>5750
                     /locus_tag="VM841_03080"
     CDS             5522..>5750
                     /locus_tag="VM841_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL89199.1"
                     /translation="MKLGLDVSQHQLTWNEIVERIRLAEDAGFDGAWVFDHFTALYGD
                     PSGPCLEGWTLLAGLAAVTERIRLGTLVTGIT"
BASE COUNT          816 a         2005 c         1990 g          939 t
ORIGIN      
        1 ctcgaccggc cgcgatcccg ccgtccgtcg ccgggtcata ggcgagcatg tcggcgtgga
       61 gcgagaccgg ctcgtcgagc gcgttgcgaa gcgcgatccg gatgcagtca ccgacggcgg
      121 cgcgcagcac gaacggcgcc ggcgcgtccg cgccgccgcg atcgccctcg tgcacgaacg
      181 tcgcggcctc gacgtcgccg agagccggca gcgtcgccgc gaccgcggcc gcgcggaact
      241 gccgcaccgg cgcatcctgt gggcacaccc gcaaagccgg cgtcaccgct tcgcgtcccg
      301 gcaaggcgcg aatcgagtcc tcgccgggac ccaagacacg gagcagcccc cacgatcctt
      361 cccggatctt gaacgcgcga ccgttcgtgt agacatagtc gcccggcatg cgctgcggtc
      421 ctcccgcggc cggaatcacg aggtcgaagc gctccgagat ccccagccgg accgaggaca
      481 ccggacggga cgaccggctc cacgcctcgg tgcggaacca atgcccgtcg acgtgcaagg
      541 cgtggacgtc gttggttccg gcgaccagcg ttcgcagcac gacgggatcg ccgagccgcg
      601 ctcgcacgag cggcgtctcg ggatcgcctc gcgtcgaggc gaacgcgcgt ggaggcggct
      661 cgcggcttcg cagcggctcg acccgctggt tgatcgcgct gccgctggat cgtccttcgt
      721 gcccgatcgg ctggtcgtcc tgcaggaaca gcacggcttc gcgaaacgat ccggtcacgt
      781 ccgccgagac gcgtgccttc gtcacgatgt cggcgatcgc accgctacgg atcggttccc
      841 ccgtctcggg gtcgtgatag gtcgagcccg gcggttcggc gatcagcgcg ccgaacagac
      901 cgtggcgcca cgacgcgagc gcattgacgt ggtcgtggaa gtacgcggtt ccgaactgca
      961 cgtccggata ccagcggtac cggacgaact cggccgacac gatctctccc gccggatgcg
     1021 actgcgcgag cggccggtcg agtacgagcc gagagccgtc gatgcgctcg atgcgtcggg
     1081 cttcgaatcg ggtcgtgccg actccgatcc cgacgaccgc gccgcgctgg aatcgaccgg
     1141 cgtcgtccac cgccaccgtg gccgcgcccg ccgcggccgc tcgtgcgcga acgggatcgg
     1201 cgtcgtaggg ccgcaccgac tgctcatagt tgaagccgtt gacgacgccg tcggatccct
     1261 gcacgtcgaa ctgtacgaag tgcacgtgca ggttgacctt cgagaacccg tggttgatcg
     1321 gcccgtcggg cagcgatgag gcgagcacga tgtcgacgca gtcctcgccc gcgttcgccc
     1381 ggatcgccag cggcgagcgc ttcgccgggt cggcgcgtac cgcggccgcg tcctcggcaa
     1441 gcacgtagat ctggccgttg ggatcgctga ggccggcgcg acggttcagc ggcaccgcca
     1501 ccgacaccgc gttgatcaag aacgtctgca cctcggtccc ttcggggcac aggctttccg
     1561 gcccggacgc gccgggcgcc gggggatccg gtccgctgcc gggatccagg tacggggacg
     1621 gcccgtggtt gggcgcgaac ggcggccttc ggccgaactg ggatcgcagg aacgggtagg
     1681 cgagccggcc gcttaccgga tcgaacaaga tcggttttcg catcccggga tccgacggcg
     1741 tgtgacccgg ccagctgcgg gcgtcctcgg gctcgccgac cacgacgccg ccctcgaccc
     1801 gccagtccca caccgacgcg tcgtagccgc gcggcacgcc cgcgggcggc agttgcatcg
     1861 cggcccaccc ggccgcatcg gtaaccgtgc gccgttcgcc gtgccaggtc acggtcgagc
     1921 cgtgcaacgc cggcgacggg acgcccgcac gcatgtgtcc atcgcgcccg ggcagctcct
     1981 gcagcggtgg caacgcgtcg gtggacgccg cgccgtcctg cagcgtgttg tacacgcgcc
     2041 agatgcccca catgccggcg aagtagtgat gcgccacgtg gcagtgaacg aggaaatcgc
     2101 ctgccgactg ctggcagccg ccggcgccgc attcgctttc gatgtcgaac gactccgacg
     2161 gaccaagcga ctgcgaatcg gtccgctcgg aggcctcggg cgtgagcggc ggccgcttgt
     2221 cgagcccgct gtcgaagcga gagtcctcgg ctcgcggctg gcgtcgccac cggaccgagc
     2281 cgccgtgaac gtggtggacg tggaacacct cgctgccgcc gtgcacgagg cgctgcttca
     2341 ccgggtcgcc gaggtaggcg cgcatcaccg gtgtcgcggg atcgccgaac gcatacgacg
     2401 agtaggcgag cgactcgtcg aagcgccccg tgagctgctg ctgcagccgc atccggttca
     2461 tgaacggctc gctgcggaag ttgatcgcgc gcgatcccgg ccggtacgcc gataggatcg
     2521 gatcgacgag cgggatcagg ctcccgtccg ccgcgcgaag ctgatactgc tcgtccccga
     2581 tctcgtggta gaagagcgtg agctcgcgaa acgccgcacc atcgggatgc gtgacgacgg
     2641 catcccaccc ccgtgcgccc tgcccggttc gcgggtcggt ccaggttgcg cccggcggct
     2701 cgacgaccac ggcgccgaac agtccgtgcg acgtctgtgc ccgctcgttc ccgtgcgaat
     2761 gaaagtagtg cgtgccctcc ggctcgttgc tcgggatcgc ccactcatag cgcacggtcg
     2821 cgccgacggc ggcggttgcc gacggattgg ttgcgacggc cggcgcgccg ccggtcgtga
     2881 gcgcgccccc gtgcacatgc aggctcgcgg gctcgtcgag ctcgttgcga agcgtcagcc
     2941 gcagacattc gccgggccgg acgcggatca ccggcggctg gatcgcgtcg ccttgcagcc
     3001 ctggtgtgac cgcgtagtcg ccgctgcccg cgcgcgcgtc cgcgtttcgc ttctcttcga
     3061 cgcggatggc ggcgatcgcg tcggtgaacg cgaacatccg tccgtctgga tcgtggtcgc
     3121 cgaaccggtt gagcgttacg gtgaccgcga tggcggtaag ggagacctca cggcgaggcg
     3181 acccgtccgg gcaggggccg cccgccccgg tctcgcgctc ttgcgcggcg agcgtgtatc
     3241 cctcggcggc ctcgtgaccc gcggcggcat cgccgtgcga cgcgcgtttg ggcgtgggca
     3301 atgcggccgg agccgccggt atcgaggcga tcaggatcgc cgccgcgatg gcaagacgca
     3361 gtcccccccg ggtgcgccgg gggggacgta tgccttgggt catgtggcta ggcgacggtg
     3421 atagagcctt ccatgtccgg gtggagcagg caccggaact cgtaggttcc ggccttcccg
     3481 aacttcacct gccaggtcaa cgcacccggc ggtccgaatc cgaggagaat cccggagttc
     3541 aggaatccct cgccgttcca ggtgcctgcg tcggtcacta ccggcttctc gccgggcggt
     3601 ccgctcggcg gcgagcccgt gggcccgaac gccttcgggt tgatgtgcca cgtgccgtcg
     3661 cccgctttga cgagcgactc gacggcatcc tgcggagtgt tgaacgacac cgtatgcgga
     3721 ccgaccggca ggaatttcac ggtctccccg accttggcga ttgcctttgc cggcaggaac
     3781 tcggtgacac ccgcgtcggt gccgccctcg atcagggtcg ggtcgccggt gcctgccagg
     3841 atcgagcccg ggcccatgcc ctgcttcgac ttgtcgtatg cacccgcgag cttcgtgcgg
     3901 atcgcatcga tctccgtgtc ggcttgtgtg gcgacctcgt cgggcgaagg caccgtctcg
     3961 gtcgcggcga cgaccgtgac cttgcccacc atcacgccgc gatgcagcgt gcagaagtag
     4021 ctgtaggtgc ccgcatcgat cgcagagtcg aaggtcacgc ggtacgtgga gccctcgggc
     4081 agaaacccgc tgctgtgcat cgagtacgtt ccggtgaagg tcttgggctg ggtgcggttc
     4141 tcgcacagat cttccttggg cgggtctccc gtctcgacgt agcacgggtt gatgccggcc
     4201 tgaaccgcgt cacccggacc ctccggaagc atgagcggaa tcttctgcca ctcggccggc
     4261 tcctcccgcg acgtcgggtc gagcttctcg gctccggcgt cgacgagcgt tccgaacgtc
     4321 accgagtgtg gctcgcccga ccagttcagc ttgaagtcga tcgagtcgcc ggcgcgaacc
     4381 ttcacctcac cgggaaagaa cttcgaaaag taggtgccga actcgcttgc cggcttgtgc
     4441 gagtccacct gcacggtgta cgtcgcgctc ttcttgccgt ccccgtcggg cgatcccgcg
     4501 ggcttgtcgg tcgagcacgc ggctccgacc aaagtcaacg ccaccgcgac gatcgtcgcg
     4561 ttgcgccatc ccatagctga ttcccccttc ggcgcgcgtt tccctccggt ggtccgacaa
     4621 cgggtcggtc tggaacgcgc tctcgccgag tctaatacct gtttgatgaa aggcaacatg
     4681 cattctgctc atcgccggcc gcgagggttt gatggcggct ttgcgggaag gttcctggtt
     4741 ctgcatgctt cttccccatg ggtgtgcacg atgcggccgc caccgggttc gagtccgcct
     4801 caggcgagta cgagcgggct cggcccggct atcccgcgga cgcggtcgac gcgctgatcc
     4861 gccggtgcga gatcggcccg ggatcgcgcg tgctggatct ggcgtgcggg acggggaagc
     4921 tcacccgtgc gctttccccg tccggggctc gtgtcgtcgg agcggatccc gtggacggca
     4981 tgctccgccg cctcgtcgag tcgagcgggg gcgttcccgt cgccgaggcg accgccgagc
     5041 atctgccgtt cgcctcgtcg gtgttcgacg ccgtcaccgt cgcgcaggcg ttccactggt
     5101 tcgacggtga cgccgcgctc ggcgagatcc atcgtgtgct gcggccggcg ggtggtctcg
     5161 cgctgctgtg gaacatccgc gacaccacgg tcccctgggt cgggcggctc aacgacgtgt
     5221 tcaaccgata cgagggcgac gtgatgcgct tctggcgcgg cgagtggcgc cacgccttcg
     5281 agcgctccgc gctttttact ccgctcgagc gccatgagtt ccggctcgat cagaccctga
     5341 cgcgcgaggg cgtgatcgac cgcgtgctgt cggtgtcgtt catcgcgacg ctgcccgcgg
     5401 gcgagcgtgc gcgcgtgatc gacgacgtcg ggcgcatcct cgacgagaat cccgaaacgc
     5461 gcggcagcga ccgtatcgtg ctgccgtacc tgacccatct ctatacgtgc aggaggacgc
     5521 cgtgaagctc gggctcgacg tgtcgcagca tcagctgacc tggaacgaga tcgtcgagcg
     5581 catccggctc gcggaggacg cgggcttcga cggcgcctgg gtgttcgatc atttcaccgc
     5641 gctctacggc gacccgtccg gaccgtgcct ggaaggatgg acgctgctcg ccggccttgc
     5701 ggccgtcacc gagcggatcc ggctcggcac actcgtcacc ggcatcacct
//
LOCUS       DATLFO010000094        10688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_43628, whole genome shotgun sequence.
ACCESSION   DATLFO010000094 DATLFO010000000
VERSION     DATLFO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43628"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VM841_03085"
     CDS             <1..789
                     /locus_tag="VM841_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVL89200.1"
                     /translation="ISVIDERTDRVIATTQVGPAPHGIVVDSLRGRVYVAVGETSQVA
                     VLDSRTGSIAQAPFAIPGCTGATGPWGMDLNPVTNVLYVACYSANVIVALNAATGTLI
                     RSVATGSGPLGVAVDAAANRVYLGMHGERSIRVLDGLTLATAASIPAEPGVWGLAFDD
                     VRGRLFAANYLTGTVTFIRGTTVTNVYAGFSGPEWISYDPVSNMIWIPEFKAGNRVTG
                     LDARTGQILPVLISGSKPTATAATPAGRVYSANFGTLNVSVISR"
     gene            complement(847..1629)
                     /locus_tag="VM841_03090"
     CDS             complement(847..1629)
                     /locus_tag="VM841_03090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89201.1"
                     /translation="MAPEEEILRAFDDDMRRTPPAGVEGVRIELDGRVRRAVGLDDTG
                     WFAVEWSDLDESTAEEAIARQIERFAALGRSFEWKYYDYDRPADLEQRLVAAGFVAEE
                     DESVMIGSVATLPRSDPPDGVRIIEVADEAGVDALIAVHDAAFGHPQPRYRETLLAQI
                     RAGDRTNVLVVALAGETAVSAARLEMPPGRRFASLWGGGTVPDWRGRGIYRALVSYRA
                     GIAAERGYEFLHVDASSQSRPILERLGFVRIARTTPYRYEIA"
     gene            1746..2111
                     /locus_tag="VM841_03095"
     CDS             1746..2111
                     /locus_tag="VM841_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVL89202.1"
                     /translation="MRTYEASAVSTGTFGRVLASSRGNDVLVDGPPWNGCLGEKINPG
                     ELFLAAVASCGVELVEVIARDRGLALRGARVEARGELDPENRVRDDLALFNRVSFHFT
                     LEGVSDGDGMRLVESFKGR"
     gene            complement(2117..2293)
                     /locus_tag="VM841_03100"
     CDS             complement(2117..2293)
                     /locus_tag="VM841_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89203.1"
                     /translation="MTSSHPRFPRTVYHSIRRKKSYSRVLHALFAERPARRFVQGMQV
                     TVTVTSGVATATVP"
     gene            2292..3296
                     /locus_tag="VM841_03105"
     CDS             2292..3296
                     /locus_tag="VM841_03105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL89204.1"
                     /translation="MERLGCFFLTARNFDDLAARVAAAEELGFEIAGFPHIAGRDALT
                     TLAAIGPRTSRIKLATGIVPIYTRTPVTMAQEAGTIAEATGNRFVLGIGTGHAALVES
                     WHGQTFESPLAAMRDYVTIVRSLFRDASVQHDGERFRAAFGFLGFRPPESIPIMIGAL
                     GPKMLRLAGELADGAILWLSSPQHIREVVVPNLRAGAAAGSRSAHELSIFPCLFAAPG
                     PDRASARDAIRRQLVPYLTLPFYRDMLIASGFGDDLEGFADRLGDGDLPGALSALSDR
                     MIDSIAATGSPEQIAETLYAFVEAGATMPGVGVVGGYEGYAGPIESLRRIRHAHTLAS
                     "
     gene            complement(3354..4163)
                     /locus_tag="VM841_03110"
     CDS             complement(3354..4163)
                     /locus_tag="VM841_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL89205.1"
                     /translation="MRTISLTPEITVLQSATEIPMLGVLPVNSFLIKGDEPILIDTGM
                     TPDKEEFLATLGSLIDPADLRWIWLTHADRDHTGAISELLELAPNARVATAFITLGLM
                     LAGNQPIPPERAYLVRTGTVVEAGSRSLEAIRPPLFDNPGTVGFYDRTSGVLFSSDFL
                     GGVMPSMDHAYADDITEVPDSDVATGQLLWGSVDAPWVHNADEAKFGRDIDMVRACDP
                     KIVLSTHLPPIRGNVDAHLKTVSKLPSSAPTMLPDQAVLEAAMSEIAHGEG"
     gene            complement(4212..4784)
                     /locus_tag="VM841_03115"
     CDS             complement(4212..4784)
                     /locus_tag="VM841_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL89206.1"
                     /translation="MTPRGYDMKKRAASAEQTRRRIIEAAFALHSRKGVAATTWQDLA
                     DEAGVAAGTVYNHFRSFDDLVPACTGFGMSLFPVPGPEMLDGLRSRKARCEKLVRTLF
                     SSYAGMQGALRFAVVERHTIPIVARTMGEMAAHVDSLVRATLGESASREAIAAGNALV
                     DFRVWDALRERELPEEVICGIVVDSLVRLR"
     gene            4913..5482
                     /locus_tag="VM841_03120"
     CDS             4913..5482
                     /locus_tag="VM841_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89207.1"
                     /translation="MLRRVSLLLAALVAAGTVSTAAPAASIDSCDWVVLHPYGACVQV
                     ENANPLIARAFVHDPHGTSTARAGFVRSEPPGGACGDPTKTQPIPLPPLPIDHQLPPL
                     PPLTVEPPLPPLPLPALPVDCRHAMLNVYEIAVDTETPRGHAGARAVGSPAPCTSTCQ
                     YDVNVYATPMVDMSRRICYSVPGSVILSC"
     gene            complement(5486..7852)
                     /locus_tag="VM841_03125"
     CDS             complement(5486..7852)
                     /locus_tag="VM841_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVL89208.1"
                     /translation="MTLVALPVAGLVAAAMLTRAAVPSREGQVTQIMGSADLVATGGL
                     DDPELNAFRSILPAGSTVAVIESLSVSTVRDGYATTVTIEELSQDPTEPPLAGRWSLR
                     SGRVPGAVGEATVHPALLTRMGSEVGGRIEIADLRLEVVGTIVHTITTRNEIVVVGPG
                     TLPANEEPQRELLVSVPPGTDLSAIAGAKPDSIGMISRGEMMRHVGDDRRSLDATLVG
                     GTIVLLLGTGLIAAAAFVVGARRQLRAIGLLGSVGADHRHVRTAVLLGGTTLGLAGSI
                     AGATLGVIAAVLIRPHLGRVTARLVGPIDFSLLPIVIAVALGTVAATTAALLPARWAA
                     RLRIVEALAGVTAPPRPASRLVRAGLLMSLPGTVILIRGTIVRDGVQLGVGFVLVVGG
                     FLLLIPLVVAAVGRLGSRMPALLRIAARDTARHGRRTSAAIAAAALALAMPVGAAAAS
                     RSIDAKEQGRKPIDDHQMIARFEGLPEDEGRAATAIATLRAALPNAAVGTVTQAIKEP
                     YSISVQAAGLGERRYDTTLAVGLYVGDEHLLRALGAGEGIEELRRGWIVSTARGTVRD
                     GTVLLQLPPDLEGTDERLLHPAAEVDGAALNLEFLPRFVISAEAAAAQGFTAGATGLI
                     LIRTPKPLTPDDVRRARAAAATVDGLAIQTAEDMAPGSRQFRGIAVAGGIAVALIVVA
                     VTVALVGAEARRDAAILAAVGAGPRLRRTLAASRAGLIAMCAAILAVPAGFVPAAIVQ
                     AARVDPYPIVVPWLTLALVCLATPAVAAVVAGIGSRRPQPVTTLRPPA"
     gene            complement(7927..8652)
                     /locus_tag="VM841_03130"
     CDS             complement(7927..8652)
                     /locus_tag="VM841_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89209.1"
                     /translation="MKPILSLEGVSRIHGSGRTKVDALIDVTLHLEPGEMVAIMGPSG
                     SGKSTLLSLAGGLDAPTTGRVLIDGVDLASLGGTELAAMRRRRLGYVFQQFNLIESLT
                     AAENVSLPLELDGVPVRAARVAAGQALERVGIESLADRFPDELSGGEQQRVAIARATV
                     GERTFLLADEPTGALDSMTGEAVLRLLRGHADAGGSGLLVTHDARYAAWADRVVFLRD
                     GRVAGQTERPPAAESLLRGPGSR"
     gene            complement(8649..9224)
                     /locus_tag="VM841_03135"
     CDS             complement(8649..9224)
                     /locus_tag="VM841_03135"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVL89210.1"
                     /translation="MSIAMSLLALLDEGPTYGLDLRNEFEARTGGVWPLNVGQVYTTL
                     ARLERDGLVRQLPHNGDVQKRYEITGDGRTRLGEWFASPCETEQPSRDELVVKLVMAA
                     WRGGRDGTRVIDAERRSAVETLQRYTALKREAPRDGDVGWPFLLDSLIFQAEARVRWL
                     DACEARLRAAGVPPGRRRVAATLPTDAEVRT"
     gene            complement(9452..>10688)
                     /locus_tag="VM841_03140"
     CDS             complement(9452..>10688)
                     /locus_tag="VM841_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL89211.1"
                     /translation="QNAPRKGVRGRGRRSVLLVAGAEFVESLVGVGAARVRDLFRRVR
                     AAAPAIVFIDELDAAGRKRGAGGGQGGTDEREQTLNQLLVEMDGFEASAGIVVIGATN
                     RPDILDPALLRPGRFDRHVTIDQPDRDGRREILRLHAADRRLDAGVDFELLARRTPGF
                     SGAELANAVNEAALLAIRGGKPAVGIEEMLEGIERVLAGPQRRGQAMTAMQRRRIATH
                     EAGHAIVAGALARPEDVHRVSILRRGRTLGSVAHDGGEDAFVTRTQILDRLAVAMAGT
                     AAEEMLLGEPSSGAEGDLERAGLLALDLVARYGMSETLGKARLLAADSEAFLGDHPPL
                     GDVAAGTREAIDAEIRELLAGAAQRAAALIETNRDVMDRIVSRLETEETLDGPALQAE
                     LRALRPAAAPPSRKRKARA"
BASE COUNT         1600 a         3823 c         3729 g         1536 t
ORIGIN      
        1 atctcggtca tcgacgagcg caccgatcgc gtgatcgcca ccacgcaggt cggccccgct
       61 ccccacggca tcgtggtcga ttcactccgc ggccgcgtct acgtcgccgt cggcgagacg
      121 agccaggtcg cggtgctcga ctcgcgcacc ggatcgatcg cgcaagcgcc gttcgcgatt
      181 cccggatgca cgggcgcgac cggcccgtgg ggcatggatc tcaaccccgt caccaacgtg
      241 ctctacgtcg cgtgctactc ggcgaacgtg atcgtcgcgc tcaacgcggc gaccggcaca
      301 ctcatccgtt cggtcgcaac ggggagcggt ccgctcggcg tcgcggtcga tgcggctgcc
      361 aaccgcgtct acctcggtat gcacggcgag cgttcgatcc gggtgctcga cgggctgacg
      421 ctggcgacgg cggcgtcgat cccggccgag cccggcgtgt ggggcctggc gttcgacgac
      481 gtgcgcgggc gcctgttcgc cgcgaactat ctcaccggca cggtcacctt catccgcggc
      541 acgacggtca ccaacgtcta cgccgggttc agcgggccgg agtggatctc ctacgacccc
      601 gtctcgaaca tgatctggat ccccgagttc aaggcgggca atcgcgtcac cggcctcgac
      661 gcccgcacgg gccagatcct gcccgtgctc atctcgggct cgaagccgac ggcgacggcg
      721 gccacgccgg cgggacgcgt ctacagcgcg aacttcggaa cgctgaacgt gtccgtgatc
      781 tcgaggtagc gacctgcagc ggtggagcgg ccgccggaag atgagggtcc ggcggccgcc
      841 accgcctcag gcgatctcgt atcgatacgg ggtcgtgcgg gcgatccgca cgaacccgag
      901 ccgctccagg atcggccggc tctgcgagct cgcatccacg tgcaggaact cgtatccccg
      961 ctccgccgcg atgccggcgc ggtaggacac gagcgcacga tagatgccgc ggccgcgcca
     1021 atccggcacc gtgccgccgc cccacaggct cgcgaagcgg cggcccggcg gcatctccag
     1081 ccgggcggcg ctcacggccg tctcgccggc aagcgccacc acgagcacgt tggtccggtc
     1141 gccggcccgg atctgcgcga gcagcgtctc gcgatagcgc ggctgcgggt gaccgaacgc
     1201 ggcgtcgtgc accgcgatga gcgcgtccac gcccgcctcg tcggcgacct cgatgatgcg
     1261 cacgccgtcg ggcggatcgc tccggggaag cgtcgcgacg gatccgatca tcaccgactc
     1321 gtcctcctcg gcgacgaagc cggcggcgac caggcgctgc tccaggtcgg ccggacggtc
     1381 gtaatcgtag tacttccatt cgaacgagcg gccgagcgcg gcgaagcgtt cgatctgccg
     1441 ggcgatggcc tcctccgcgg tcgattcgtc gagatcggac cactcgaccg cgaaccagcc
     1501 ggtgtcgtcc aggccgacgg cccggcgaac ccgcccgtcg agctcgatcc gcactccctc
     1561 gacgccggcg ggcggcgttc gtcgcatgtc gtcgtcgaac gcccgcagaa tctcttcttc
     1621 cggtgccatc gtcatctccg ttcgacggcg aagacgccgc cgtcgcgttc gtagaaagcc
     1681 acgcgccgca ggctacatca gctgttgcgc acacgcgccg gccgccgccg tgcttcaatg
     1741 atcgcgtgcg aacctacgag gcgtcggcgg tatcgaccgg aacgttcggc cgcgtcctgg
     1801 cctcgtcccg tggcaacgac gtgctcgtgg acggcccgcc gtggaacggc tgcctcggcg
     1861 agaagatcaa tcccggcgag ctgttccttg cggcggtcgc ctcgtgcggc gtcgagctgg
     1921 tcgaggtgat cgcgcgcgac cgcggcctcg cgctgcgcgg cgcgcgcgtg gaggcgcgcg
     1981 gcgagctcga ccccgagaat cgggtgcgcg acgacctcgc gctgttcaat cgcgtctcct
     2041 tccacttcac gctcgaaggc gtcagcgacg gcgacggcat gcggctggtc gagtcgttca
     2101 agggccgctg accgctctac ggaacggtcg cggtcgcgac ccccgaggtg acggtcaccg
     2161 tcacctgcat cccctgaacg aagcgccggg cgggccgctc cgcgaacagc gcgtgcagta
     2221 ctcgcgagta gctttttttg cggcgaatcg agtggtagac agtgcgcggg aatcggggat
     2281 gggaggatgt catggaacgg ctcggctgct tctttctgac ggcgcgcaac ttcgatgatc
     2341 tcgcggcccg ggtcgccgcg gccgaagagc tcggcttcga gatcgcgggc tttccgcaca
     2401 tcgcgggccg tgacgcgctg acgacgctcg ccgcgatcgg gccgcgcacc tcgcgtatca
     2461 agctcgccac cggcatcgtg ccgatctaca cgcgcacgcc cgtgacgatg gcgcaagaag
     2521 cgggcacgat cgcggaagcg accggcaacc gcttcgtgct cggcatcggc acgggccatg
     2581 ccgccctggt cgaatcatgg cacggccaga cgttcgaaag cccgctcgcc gccatgcgcg
     2641 actacgtgac catcgtgcgt tcgctgttcc gcgacgcgtc ggtccagcac gacggcgagc
     2701 gcttccgcgc ggccttcggg ttcctgggat tccgtccgcc ggagagcatc ccgatcatga
     2761 tcggggcgct cgggccgaag atgcttcggc tcgcgggcga gctcgccgac ggcgcgatcc
     2821 tgtggctgtc gtccccgcag cacatccgcg aggtcgtggt cccgaacctg cgcgccggcg
     2881 ccgcggccgg ctcgcgcagc gcgcacgagc tctcgatctt cccgtgcctg ttcgccgcac
     2941 ccggcccgga tcgcgccagc gcgcgggacg cgattcgccg ccagctcgtt ccgtacctga
     3001 cgctgccttt ctatcgcgac atgctgatcg cgagcgggtt cggcgacgac ctcgagggtt
     3061 tcgccgatcg gctgggggac ggcgaccttc cgggtgcgct gtcggcgctg tcggaccgca
     3121 tgatcgactc gatcgcggcg accggctcgc ccgagcagat cgccgagacg ctgtacgcct
     3181 tcgtggaagc cggcgcgacc atgcccggcg tcggcgtggt gggcggctac gaaggctacg
     3241 cgggaccgat cgagagcctg cgccggatcc gccacgcgca cacgctcgca tcgtgacggc
     3301 tgacacgggg ccgcccggcc ttcccgcggc cccgtgtcgt cgtgcgcggc gattcagcct
     3361 tcgccgtgcg cgatctcgct catcgccgcc tccagcacgg cctgatccgg cagcatcgtg
     3421 ggcgcggacg acggcagctt cgagaccgtc ttgaggtgcg cgtcgacgtt gccgcggatc
     3481 ggcggcaggt gcgtggagag cacgatcttc ggatcgcacg cgcgcaccat gtcgatgtcg
     3541 cgtccgaact tggcctcgtc ggcattgtga acccagggcg cgtccacgct gccccacagc
     3601 agttggccgg tcgcgacgtc gctgtccgga acttcggtga tgtcgtcggc gtaggcgtga
     3661 tccatcgacg gcatcacacc gccgaggaag tccgagctga acagcacgcc cgacgtgcgg
     3721 tcgtagaagc cgaccgtgcc ggggttgtcg aacagcggcg ggcgaatcgc ctcgagcgac
     3781 cgggagccgg cttcgacgac ggtgccggtt cgaacgagat aggcgcgctc gggcgggatc
     3841 ggctggtttc ctgccagcat cagaccgagc gtgatgaacg cggtcgcgac gcgcgcgttc
     3901 ggcgcgagct cgagcagctc gctgatcgcg ccggtgtggt cgcggtcggc gtgcgtgagc
     3961 cagatccaac gcagatcggc cggatcgatc agcgatccga gcgttgccag gaactcctcc
     4021 ttgtcggggg tcatgccggt gtcgatcagg atcggctcgt cacccttgat caggaacgag
     4081 ttgaccggca gaacgccgag catagggatc tcggtcgccg attgaaggac ggtgatctcg
     4141 ggcgtgaggg atatcgtgcg cattcctgct cccctggtat cgcgtgacga cgtggtgtga
     4201 gcccgggctg cctatcgcag ccggacgagc gaatcgacga cgatgccgca aatcacctcc
     4261 tccggcagtt ctcgctcgcg cagcgcgtcc cagacgcgaa agtccacgag cgcgttgccc
     4321 gcggcgatcg cctcgcggga cgcgctctcg ccgagcgtgg cccgcacgag ggagtcgacg
     4381 tgcgcagcca tctcacccat cgtgcgcgcg acgatcggaa tggtgtgacg ctccacgacc
     4441 gcgaagcgca acgctccctg catgcccgcg taggacgaga acaaggtgcg gacgagcttt
     4501 tcgcatcgag cctttcgcga ccggagcccg tcgagcatct cgggaccggg cacgggaaac
     4561 aacgacatgc cgaagccggt gcacgcgggc accagatcgt cgaagctgcg gaagtggttg
     4621 tagacggtgc cggccgccac ccccgcctcg tcggccaggt cctgccacgt ggtggccgcc
     4681 acccccttcc gggagtgcag cgcaaaggcg gcctcgatga tccgccgccg cgtctgctcg
     4741 gcggacgcgg cccgcttctt catgtcgtac ccgcgcggag tcattgagtg aattctcact
     4801 caatgaatta gaacccgtca aacgcggacc gccgaagcaa gcaggagcca ggacgaactc
     4861 ggcgaatggg tacggcacga cctttctgcc gatcagcatc caggaggaag acatgctgcg
     4921 tcgcgtttcg ctgctgctgg ccgcgctcgt cgcagccggc accgtctcca cggcggcccc
     4981 ggccgcatcg atcgactcgt gcgactgggt ggtgttgcac ccgtacggag cgtgcgtcca
     5041 ggtggagaac gccaatccgc tcatcgcgcg ggcattcgtc cacgacccgc acggcacctc
     5101 caccgccagg gccgggttcg tgcgctccga gccgcccggg ggggcgtgcg gggatccgac
     5161 gaagacccaa ccgattccgc ttcccccgct cccgatcgac catcagctcc cgccgctccc
     5221 gccgctgacg gtcgagcctc cgctgcctcc gctgcctctg ccggcgctgc cggtggactg
     5281 ccgccacgcg atgctcaacg tgtacgagat cgccgtggac accgagacgc cgcgcggcca
     5341 tgccggagca cgcgccgtcg gatcgccggc gccgtgcacg agcacgtgtc agtacgacgt
     5401 caacgtctat gccactccga tggtcgatat gagccggcgg atctgctact cggttcccgg
     5461 gagcgtcatt ctgagctgct gacgcctacg cgggcggccg aagcgtcgtc acgggctgcg
     5521 gtcgtcgcga cccgatcccc gcgaccacgg cggcgacagc gggcgtggcc agacaaacca
     5581 gcgcgagggt cagccaggga acgacaatgg ggtacgggtc cacacgcgcc gcctgcacga
     5641 tcgctgcggg cacgaacccg gcgggcacgg cgaggatcgc ggcacacatg gcgatcagcc
     5701 ccgcgcggga tgcggccagg gtccgacgaa gccgcggccc ggcacccacg gccgcgagga
     5761 tcgccgcgtc tcggcgcgcc tccgcgccga cgagcgcgac ggtgaccgcg acgacgatca
     5821 acgcgaccgc gatcccgccg gccacggcga tgcctcggaa ctgccggctg ccgggagcca
     5881 tgtcctcggc ggtttggatc gccagaccgt cgacggtcgc ggcagcggcg cgcgcgcgcc
     5941 gcacatcgtc cggcgttaac ggcttcggcg tccgaatcag gatcagcccg gtcgctccgg
     6001 cggtgaaccc ttgcgccgcc gcggcctcgg ccgagatgac gaagcgagga aggaactcga
     6061 gattgagcgc ggctccgtcc acctcggcgg cggggtgcaa cagccgctcg tccgttccct
     6121 cgaggtcggg aggaagctgc aagagcacgg tgccgtcgcg aacggttccg cgcgccgtcg
     6181 atacgatcca tccgcgacga agctcctcga tcccctcccc ggcgccgagc gcgcgaagca
     6241 gatgctcgtc accgacgtag agcccgaccg cgagcgtcgt atcgtaccgc cgctccccca
     6301 gcccggctgc ctgcaccgag atcgagtacg gctctttgat cgcttgcgtg accgtgccca
     6361 cagcggcatt tggaagcgct gcccgcagcg tcgcgatcgc ggttgcagct cggccctcgt
     6421 cctccggaag tccttcgaag cgagcgatca tctggtggtc gtcgatgggc ttgcggcctt
     6481 gctccttggc gtcgatgctc cgcgatgcgg cagccgcccc aacgggcata gcgagcgcga
     6541 gcgccgcggc cgcgatcgca gcggaggtgc gacggccgtg ccgcgcggta tcccgcgccg
     6601 cgatccgcag cagggccggc atgcgggagc caaggcgccc cacggcggcg acgacgaggg
     6661 ggatcagcag caagaagccg ccgacgacca gaacgaaccc gaccccgagc tgcaccccgt
     6721 cgcgcacgat cgtgccacga atcaggatca cggtacccgg cagggacatg agcagcccgg
     6781 cccgcacgag gcggctcgcc ggtcgaggcg gggcagtgac gccggcgagc gcctcgacga
     6841 tgcgaagcct cgccgcccag cgcgccggga gcagtgccgc ggtggtcgcg gcgacggtgc
     6901 ccagcgcgac ggcgatgacg atcggcagca acgagaagtc gatgggaccg accaggcgcg
     6961 cggtgacgcg accaaggtgc ggccggatga gtaccgcggc gatcacaccc agcgtcgcgc
     7021 cggcgatcga gccggccaga ccgagcgtcg ttccgccgag caagaccgcg gtgcgcacgt
     7081 gcctgtggtc cgctccgacc gatccgagca gcccgatggc gcgcagctgc cgccgcgcgc
     7141 cgacgacgaa cgcggccgca gcgatgagcc cggtgccgag cagcaggacg atggttccgc
     7201 cgacgagcgt cgcgtccaag cttcggcggt cgtcgccgac gtggcgcatc atttcgccgc
     7261 gcgagatcat gccgatcgag tccggcttcg cgcccgcgat ggccgacagg tccgtgccgg
     7321 gtggaaccga cacgagcagc tcgcgttgtg gctcttcgtt ggcggggagc gtgcccggcc
     7381 cgacgacgac gatctcgttc ctcgtcgtga tcgtgtgcac gatcgtaccg acgacctcga
     7441 ggcgcagatc ggcgatctca atccgtccgc cgacttcgct tcccatcctc gtgagcagcg
     7501 ccggatgcac ggtagcctca ccaaccgcgc ccggcactcg tccgctgcgc agcgaccatc
     7561 gcccggcgag cggcggctcg gtgggatcct gcgacagctc ttcgatcgtc acggtcgtgg
     7621 cgtaaccgtc gcgcaccgtc gacaccgaga gcgactcgat caccgcgacg gtggatccgg
     7681 cgggcaggat cgagcggaac gcgttcagct ccgggtcgtc gagcccgccc gtggcgacga
     7741 gatcggctga tcccatgatc tgggtaacct gaccctcgcg cgacggaacg gccgcgcgcg
     7801 tcagcatcgc cgcggcgacc aggccggcga ccggcagggc gacgagcgtc atgatcaacg
     7861 cgctgcggcc gggtgcgcgg gccgcggagc gccacgcgta acgcgcgaga gcgcggcgcg
     7921 acgacgtcac cgcgaccccg gtccgcgcag gagcgattcg gccgcgggcg ggcgctcggt
     7981 ctggccggcg acgcgcccgt cgcgcaagaa cacgacccgg tcggcccatg ccgcgtagcg
     8041 agcgtcatgc gtgacgagca ggccgctgcc accggcatcg gcgtgaccgc gaaggagccg
     8101 cagcaccgcc tcgcccgtca tcgaatccag tgcgcccgtt ggctcgtcgg caagcaggaa
     8161 cgtgcgctcg cccaccgtcg cccgcgcgat cgccacgcgc tgctgctccc cccccgaaag
     8221 ctcgtcggga aagcgatccg ccagggactc gatgccgacg cgctcgagcg cctggccggc
     8281 tgcgacgcgc gccgctcgca cgggcacacc gtcgagctcg agtgggagcg acacgttctc
     8341 ggccgcggtc agcgactcga tcaaattgaa ctgctggaag acgtagccga ggcggcggcg
     8401 gcgcatggcc gccagctccg tccccccgag ggaggcgaga tcgaccccgt cgatgagcac
     8461 gcggccggtc gtgggagcat cgaggccccc ggccagcgac agcaaagtgg acttgccgga
     8521 tccgctcggc cccatgatcg cgaccatctc gccgggctcg aggtggaggg tgacgtcgat
     8581 gagcgcatcc accttcgtgc ggccggagcc gtggatccgc gagacgcctt ccaaggacag
     8641 gatcggtttc acgttcgcac ctctgcgtcg gtggggaggg tcgcggcgac gcgacgccgg
     8701 ccgggcggga cgccggccgc acgcaaccgc gcctcgcagg cgtcgagcca tcgaacccgg
     8761 gcctcggcct ggaagatcag cgagtcgagc aggaacggcc agccgacgtc gccgtcgcgc
     8821 ggcgcctccc gtttgagcgc ggtgtatcgc tggagcgtct cgaccgcgct gcgacgctcg
     8881 gcgtcgatga cgcgggtccc gtcgcggcca ccgcgccagg ctgccatcac cagcttcacg
     8941 accagctcgt cacgggacgg ctgctcggtc tcgcagggcg aggcgaacca ctcgccgagc
     9001 cgcgtgcgcc cgtcgccggt gatctcgtat cgcttctgaa cgtcgccgtt gtggggcagc
     9061 tgccgcacca ggccgtcgcg ctccagacgc gccagagtcg tgtagacctg accgacgttg
     9121 agcggccaga cgccgcccgt gcgggcctcg aactcgttgc gcaggtcgag accgtatgtc
     9181 ggaccttcat cgagcagcgc gagcaagctc atggcgatgg acatcgaggg gagagtcctt
     9241 tccgagccgc ccgaatccga gccgccgggc gagggtcgcg ccgaactata tactcggtat
     9301 actcttgcag tcaagcagcc ccacgagggc cggttccctt gaaaacgagc agtcgacgtg
     9361 acagcgatgc tgcatcgtcc acgcgtgacg ccgcggcatc aacacgccgt ccatggaccg
     9421 atgcgtggga tcccggaacc cggcctctag gctaggcgcg tgccttgcgc ttgcgggacg
     9481 ggggcgcggc ggcgggccgg agcgcacgca gctcggcctg cagcgccggg ccgtcgagcg
     9541 tctcctcggt ctcgagccgc gacacgatcc ggtccatcac gtcgcggttg gtctcgatga
     9601 gcgcggcggc gcgttgcgcc gcaccggcga gcagctcgcg gatctcggcg tcgatcgcct
     9661 cacgcgtgcc ggccgcgacg tcgccgagcg gcggatggtc acccaggaac gcctcggaat
     9721 cggccgccag cagcctcgcc tttccgagcg tttcgctcat gccgtagcgc gcgacgagat
     9781 cgagtgcgag cagcccggcg cgttcgaggt ctccttccgc gccgctggac ggctcgccga
     9841 gcagcatctc ctcggccgcg gtgccggcca tcgccaccgc gaggcggtcc aggatctggg
     9901 tgcgtgtcac gaacgcgtcc tcgccgccgt cgtgcgcgac cgagccgagc gtgcgcccgc
     9961 gacggaggat cgaaacgcgg tgcacgtcct cggggcgggc gagtgcgccg gccacgatcg
    10021 cgtgcccggc ctcgtgcgtg gcgattcggc ggcgctgcat cgcggtcatc gcctggccgc
    10081 gtcgctgcgg cccggcgaga acccgctcga tgccttcgag catctcttcg atgccgaccg
    10141 cgggcttgcc gccccggatc gcgagcagcg cggcttcgtt gacggcgttg gcaagctcgg
    10201 cgcccgagaa gccgggcgtg cgccgcgcga gcagctcgaa gtcgacgccg gcgtcgaggc
    10261 ggcggtccgc ggcgtgcagg cgaaggatct cgcggcgtcc gtcgcggtcc ggctggtcga
    10321 tggtcacgtg acggtcgaag cgtccggggc gaagcagcgc cggatcgagg atgtcgggcc
    10381 ggttggtcgc gccgatcacc acgatgccgg ccgaggcctc gaacccgtcc atctcgacga
    10441 gcagctggtt gagcgtctgc tcgcgctcgt cggtgccgcc ttgtcccccg ccggcgccgc
    10501 gcttgcggcc cgcggcgtcg agctcgtcga tgaagacgat cgcgggcgcg gccgcgcgca
    10561 cgcgacggaa cagatcgcgc accctcgcgg caccgacgcc gacgagcgac tcgacgaact
    10621 cggcgcccgc cacgagaaga acggaacgtc ggcctcgccc gcgcacgcct ttgcgaggag
    10681 cgttttgc
//
LOCUS       DATLFO010000095        16682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_52558, whole genome shotgun sequence.
ACCESSION   DATLFO010000095 DATLFO010000000
VERSION     DATLFO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52558"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VM841_03145"
     CDS             <1..276
                     /locus_tag="VM841_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVL89212.1"
                     /translation="ALCVAHADPSGSPVPVTASWAYFRLHGGPGYRQYTPEENEAIAR
                     VVAGVAATVDPVFVFFDNDTGGAAVRDAQTLTANLAVTGVDGRDEVR"
     gene            373..717
                     /locus_tag="VM841_03150"
     CDS             373..717
                     /locus_tag="VM841_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVL89213.1"
                     /translation="MDQPLRSVADEPRQSVRMTADRVLVRKPDEGERTSKGGLLIPAT
                     ATSVSKRCQWSEVVAVGPSVRSIEPGDQVLHLPESGLEVDIRGEEFLLLRERDLHAVA
                     SDRSDGGTGLYL"
     gene            818..1813
                     /locus_tag="VM841_03155"
     CDS             818..1813
                     /locus_tag="VM841_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HVL89214.1"
                     /translation="MRTRLIAVAAALASFAAACSSGGSPDSSGRVRIVTTVSPITNIV
                     AWVGGERVSVTGIVPEGTNSHTFEPRPSDARLVAEADVIFVNGLGLEEPTLELAEPNR
                     KEGTEIVKLADRTIDRSEWIFDFSFPESGGDPNPHLWTNPPYAKRFAQIVRDTLTTLD
                     PEGVALYDRNYLSFAERIDELDTAVRAATLSVPEKNRKLLTYHDSFPYFAREYGWTIV
                     GAVQPSDFSEPTPREVSRLIQQIRDEGVPAIFGSEVFPSPVLEQIARESGATYVAELR
                     DDDLPGSKGDPAHSYFGLMVFDFKTFMKALGGDVTVFDTIDTANPDDESNVTYRE"
     gene            1810..2595
                     /locus_tag="VM841_03160"
     CDS             1810..2595
                     /locus_tag="VM841_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HVL89215.1"
                     /translation="MSDLIRLENVTCSYGGEPVLSHVDLTVHAGAFVGVVGPSGSGKS
                     TLLKAITGSVKPVAGRILRPGGPVTVGYVPQVETVNWYFPVTVLEVVLMAATGRRLPW
                     PSIAERRLAHDTLERLGLDAYASRHIRELSGGQQQRVFIARALFARPQLLLLDEPTSG
                     VDVHTRHDVMHLLHDLHHDGLAILLTTHDLNGVAAHLPELVCLNREVVATGAPGDVLR
                     PDVLERTYGAPMEVLSHGGMPVVVESVPPDELHGKPGHHEHSA"
     gene            2592..3434
                     /locus_tag="VM841_03165"
     CDS             2592..3434
                     /locus_tag="VM841_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HVL89216.1"
                     /translation="MSTLLEPLELAFFRNGMIVATLAGALCAMVGVYVVLRGMSYIGH
                     GLSHAIFGGAVISFVAKVNFTIGAAAWGFLSALAINRVARRRSIGADAAIGVITTASF
                     AIGIAIISRASSLRRNFDAALFGNVLGVGPGDIVVVAAVLTVATAMVFVLYRPLLFIT
                     FDPEVAEASGVRTGAVDAMFSLVLAATIVATMNVLGVLLIAAALVIPPVVARMLTNSF
                     RRMLWLSIAIGAAGGLIGMYLSYFVDMASGPAIVIVESAAFVVVYALSGMRGGRILPA
                     GAVD"
     gene            3434..4405
                     /gene="mshD"
                     /locus_tag="VM841_03170"
     CDS             3434..4405
                     /gene="mshD"
                     /locus_tag="VM841_03170"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HVL89217.1"
                     /translation="MTTLPHDHDAAHASDVALIDLADPAERTRIHALMGACEAADGHL
                     PFGDHELLAIQTGAVEDHIGFAVHDEGTLAGYGHLSRLGDAWFFEIAVHPEMRGRGIA
                     TRILDAIVAHTAAHGGGTLSTWAYRQAPDVGGLIRHAGLRPSRTLLQMRLNALPAAAP
                     ALPDGLVLDRFCPGIDDDEWLALNARAFAHLPDQGAWTRRDLHARISADWFDAEDVLT
                     VRRDGTLIATCWTKLDPTTRTHDGRQLGEIYVIAVDPAEAGAGLGRILATVGLDHLRE
                     RGAQVGMLYADKDNEAAVAMYESLGFLVNHEDVCLITDVAPSGATSG"
     gene            complement(4368..4856)
                     /locus_tag="VM841_03175"
     CDS             complement(4368..4856)
                     /locus_tag="VM841_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVL89218.1"
                     /translation="MKGARPSDHEHELDNLDEVLRSHGLRRTPQRQATLDAIAASDGH
                     ATAEQIVARVRKRLPAVSPSTIYRTLTSLEEVGVVCHAHLGHTASVYHLGGGGLHQHL
                     VCERCEAIIEVDDAVVAPFAKRLADRYGFRADFTHFAVLGECASCAKRPNRSSRRTAP
                     RR"
     gene            complement(4897..5514)
                     /locus_tag="VM841_03180"
     CDS             complement(4897..5514)
                     /locus_tag="VM841_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HVL89219.1"
                     /translation="MPFPTRLLTEDEHVILDVRPHWKALIEPMLSTMAIVTVAAVLIV
                     KIGPARPAALRWAVAGAAVMVWIWAAGLQLARWKFTEFVLTNERIIARSGVIAKVSRE
                     IPLERVNDVTVTQSMLDRVLRSGDIRLESAGEYGQTTFDDIGHPVEVQKQIYEAAEGR
                     RGLGPDLIHHPASVADELAKLADLRDRGALTAEEFDARKKRLLER"
     gene            5653..6285
                     /locus_tag="VM841_03185"
     CDS             5653..6285
                     /locus_tag="VM841_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVL89220.1"
                     /translation="MIKGLLVDFGGVLTTSVIDAFAEFARGEGLDPKDLAAVMREHMQ
                     APDSFFLRVERGHIDTADFEIELAGAIRAKLGVEIASTGLKSRLFAGSRPNEPMLDAL
                     RRARANGVRTALVSNSWGVTEGGYPVHQFDELFDAIVISGEVGLRKPDPEIYLLAAER
                     IAAEPPQCIFVDDFAINVEGAVATGMAGVHHVETDATLRELEAFLSVPLR"
     gene            complement(6282..6554)
                     /locus_tag="VM841_03190"
     CDS             complement(6282..6554)
                     /locus_tag="VM841_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89221.1"
                     /translation="MNEVTITPRAAEVLRRGLEAARLDPSAFGVRVWISAGQARSGFA
                     DEPDEGDALVDAGGLRVFVDPSVTANGPAAIDVSAEHDQIVVVPAG"
     gene            6597..7094
                     /gene="greA"
                     /locus_tag="VM841_03195"
     CDS             6597..7094
                     /gene="greA"
                     /locus_tag="VM841_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HVL89222.1"
                     /translation="MSTDQQPPVLSPAALERLREEHERLTTDGRRDISERLLRARELG
                     DLSENAEYDQTKNDQAQMEARIRYLEWTIKNAIVQEGPISTDSVGPGMVVTLRSLDSD
                     EEPEQYLLAQSKEERAKGMRTITTVSPLGQAIVGRGKGDQISYEAPGGTFRYEVVAYE
                     PWDGS"
     gene            7123..7635
                     /locus_tag="VM841_03200"
     CDS             7123..7635
                     /locus_tag="VM841_03200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL89223.1"
                     /translation="MTMYATFKTSMGDIKCKLFPDHAPKTVENFVGLATGKQTWKDPQ
                     TGAQKNDPLYDATIFHRVIPEFMIQGGDPMGTGTGGPGYRFEDEVDDGPSFTRAGYLA
                     MANAGPNTNGSQFFITEIPTPWLTGKHTIFGEVVEGMEVVNAIARVERGANDRPREDV
                     VLQTVEISES"
     gene            7632..7853
                     /locus_tag="VM841_03205"
     CDS             7632..7853
                     /locus_tag="VM841_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89224.1"
                     /translation="MSRTVTISRRAAWLLVGAAVWTLYVWITRIWIVAGQNQSTSFKV
                     VHYVLAAVSIVFGAAVGAIGVRALRRARA"
     gene            7850..8629
                     /locus_tag="VM841_03210"
     CDS             7850..8629
                     /locus_tag="VM841_03210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVL89225.1"
                     /translation="MNAELDAAARAAREAGALLLERFRGPARGVGTKSSGTDMVSDAD
                     RDAENVITLTLREAFPADAIFGEETGVHDGNSGRTWIVDPLDGTTNFLYGIPQWCVSI
                     ACEDERGGVAGVVYDPARDELFAAARGGGATLNGSPVHASTLSDVSKALVATGYGYEP
                     SERERWGGVIARLLPAVRDVRRAGSAALDLAWTAAGRVDAYAEVPCRHWDRAAGVLIA
                     AEAGCEISMLDPIGPAGDGVIAAPAALHEPLRAAIEEALSA"
     gene            8626..8988
                     /locus_tag="VM841_03215"
     CDS             8626..8988
                     /locus_tag="VM841_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HVL89226.1"
                     /translation="MSPDVALLYDDNCGFCRRAVRMVLAWDRHGRIRAVPLQAPEALE
                     LLPDMTEAQRMDSWHLVVGGRVYSAGAATGPLMRLLPAGAPGAVIAETFPRGTERAYR
                     WIAANRERLGRFLPGAGG"
     gene            8985..9167
                     /locus_tag="VM841_03220"
     CDS             8985..9167
                     /locus_tag="VM841_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89227.1"
                     /translation="MTQVATLLFVVLLVMQFSMMKWEMMALQRHGRPLWLALIPIVSL
                     LVIGYDAIAGRVRRRG"
     gene            complement(9164..11020)
                     /locus_tag="VM841_03225"
     CDS             complement(9164..11020)
                     /locus_tag="VM841_03225"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HVL89228.1"
                     /translation="MDRQAFRERLTRGTLLGDGGLGTSLVAAGVPLDSFFDELNLSNP
                     AVVEQAHRSFVFAGADFVETNTFGANRYKLTAHGLEERIRELIVAAVGIARRAGVYVA
                     GSMGPLGVRLAPYGRVRPADARDAFAEQAGLLAEAGADFLIVETHADLAEAEEAVRAA
                     LETGLPVVATMSFTRDDRTLLGSTPEECAAHLATLGVDALGVNCSEGPEQVLRLLETM
                     RPHAQGIPLTAQPNAGGPSRMSGRLFYPATPEYFADFARQAIAAGVAIVGGCCGTDAG
                     HIRAMAGSRAAGGARAAAASATEPLPAEEETEPLPAPFAGKPAASRFAATLTSGRLVI
                     TVEMDPPRGSSLARMLAGADTLRQAGADAIDVADSPMARMRMSPWAACHLIQDEAGIE
                     TVLHFPTRGRNLLRVQGDLLAAYALGVRNIFACMGDPTAIGDYPNAADTMDVVPSGLV
                     SLVKSGFNEGLDRTGASIGEATSFVVGCALNPNAEDLEREVTVFHRKIESGADFALTQ
                     PIFDLSVLARFRAAYEAAHGPVTIPLLVGLLPLVSARHAEWLHNEVPGIDVPEAVREA
                     LRTAGDKAEATGLAIASDLSAQIAENAAGIYLMPPFGRYDLAADVIERARDL"
     gene            11102..11605
                     /gene="mscL"
                     /locus_tag="VM841_03230"
     CDS             11102..11605
                     /gene="mscL"
                     /locus_tag="VM841_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVL89229.1"
                     /translation="MSDEGFDRVRRELSESFHVLKARTRVLVDFKNFIAKGSALDLAI
                     GVVIGAAFNSVVQSLVKDVLTPLISIPGAADFSDLRWCLRNGPEDTCAVGLGYGAFVT
                     AMISFLLTAAAVFFFVVRPVNAMRGWRRGEAPKAPDTRECPECLTSIPLAATRCRACT
                     AVIEPAS"
     gene            11685..13052
                     /locus_tag="VM841_03235"
     CDS             11685..13052
                     /locus_tag="VM841_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89230.1"
                     /translation="MPEDKKKPAKKAAAKRATARKTTARKTGASKGASGRGASARSKA
                     PAKPATRKTPARKPAPGRKPAAAEESELEQSSVDRDEDDAPLEQRTSAPIVIGAGASR
                     PGEPVVAASSETVAIADAATGEAVAVTDQAPDEPPVPERYPEDIPVMVVSALLAVSVF
                     LPWYKLTSAFRQTTVTASGWETGTWGPMIFFLGLASLAIAVFRRAGVRVGLPVADSHL
                     HEGIGWAAIIGAVIKLRAVPKEQGIQMGWNWGIIVAIVAAFALAFLAGRMSGSMTFTT
                     IPGWFRGRAGKIGLAVLLLAIAGSALFGITQNVSPYALATGRPTTDGGGDGGVASNVL
                     RDEFPKCAEGFPRPADAKPREGVEQKGQSPCVFTFSTAETPDELLKFYTSELKKDGWS
                     VQALPSRGEGIGHAMTFSSPKCGSMSMQRAQGASESNVIVAVGGPCPTFQPGGSGSTP
                     TPSPS"
     gene            13073..14338
                     /gene="glyA"
                     /locus_tag="VM841_03240"
     CDS             13073..14338
                     /gene="glyA"
                     /locus_tag="VM841_03240"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVL89231.1"
                     /translation="MAAHGNVPLTEADPTVAGLIRDEEQRERSVVRLIPSENYVSRAV
                     LEATGSVLTNKYSEGYPGKRYYEGQQFIDPLETLAIERATGLFGADHANVQPYSGSPA
                     NLAVYFAFLQPGETVMGMALPHGGHLTHGWGVSITGKYFRPVQYEVRREDGRIDYDQV
                     RDLAKAERPKLLFAGGTAIPRTIDFAAFGEIAREVGAVFVADIAHIAGLVAGDAHPSP
                     LPHADVVTTTTHKTLRGPRGAMILCKQEHAAAINKAVFPGLQGGPHNHTTAGIAVALH
                     EASQPPFKDYAAQIVANAKALATALTERGYDLVSGGTDNHLILIDLTRNDVPGKIAAK
                     ALDKAGLECNYNTVPFDPRKPFDPSGIRIGTPAVTSRGMGEAEMTQIAAWMDAAIAAA
                     DDDAALAKIAGEVAALCEQFPAPGLAIGD"
     gene            14370..15191
                     /locus_tag="VM841_03245"
     CDS             14370..15191
                     /locus_tag="VM841_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVL89232.1"
                     /translation="MKRRWVHAAAIAVVAAIFVIPLVVLVTGSLRLPGQAPPRTLEVI
                     PDPVTLDAWRDAFDFSPLARSMVNSLIVAAVFVPLAVLASSWAGFAIALARERLRGRL
                     IVLALLLMMVPATALWLTRFVIFKAFGMVDTFWPLVLPALLGGSPLAVLLYAYAYGRI
                     PGELYDASRLEGLSPVRIWWRVGMPLVRATTVAVAMLAFLTTWANFIDPLLYLNSEDR
                     FTAPLTLRYLEQLGPTNWPVLLAGAAAVTAPVVVVFLLAQRFFLQEERGVGWLGR"
     gene            15176..16489
                     /locus_tag="VM841_03250"
     CDS             15176..16489
                     /locus_tag="VM841_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVL89233.1"
                     /translation="MARTMKLVALVVATLTALAACGGEDSRAGSTKQVSFQIFGDAAE
                     IKAYQSLIDAFQAKTPGVKVKLVPVPSQNDHSAKLSAGFAGGSPPDLFLINFRRFGQY
                     AARGALEPVEPMLESSTTMTESDFYPQAIDAFRFGGTLTCMPQNISSQVVYYNKELFK
                     RFNVAEPKAGWTWTDFLSTAKALTKDTDGDGTTDIYGLGVDPTLVFLGPWVWQAGAQV
                     VDDTDNPSQTVMLDDKALEALKFFIELRRIHKVVPDHAEAESEGAEARFGNGRSAMLI
                     DSRRGTTALRQQTGLDWDVVQMPKFREEATMLHSDAYCMSKASKSKDAAWKFVEFAFG
                     EEGAAIIAKTGRTVPSLKKVAESPAFLDPTQKPANAHAWLDAIPTVRRLPNISTWNEI
                     ESKANVVIEEWFYGQERIEALGIEIDLATRELFAEAKAAPSPGSS"
     gene            16497..>16682
                     /locus_tag="VM841_03255"
     CDS             16497..>16682
                     /locus_tag="VM841_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL89234.1"
                     /translation="MAGLSLRGVTKSYGEHPAVEDLTIDVADGELFVLLGPSGCGKST
                     TLRLIAGLERANTGTIRI"
BASE COUNT         2522 a         5779 c         5787 g         2594 t
ORIGIN      
        1 gctttgtgcg tggcgcacgc ggatccgtcc gggtccccgg tgccggtcac cgcctcgtgg
       61 gcctatttcc ggctccacgg cggtcccggc taccggcagt acacgccgga ggagaacgag
      121 gcgatcgcac gcgtcgtcgc gggcgtggcg gcaacggttg atccggtctt cgtgttcttc
      181 gacaacgaca ccggcggtgc cgcggtgcgc gacgcgcaga cgctgacggc gaacctcgcc
      241 gtgacgggcg tggacggtcg cgacgaggtt cggtgagcac ggagtgggac cggcccgctc
      301 cttggacgga gcaccgaccg ctcaaagaaa tgcaaaatgc gaagccccct gcgggccgtc
      361 tatgctttgc gcatggatca gccgctgcgc tccgtcgccg acgaacctcg ccagtccgtt
      421 cgcatgaccg cagatcgtgt gctcgtgcga aagcccgatg agggagagcg cacctcgaaa
      481 ggcggcctgc tgatcccggc gactgcgacg agcgtcagca agcggtgcca atggagcgaa
      541 gtcgtcgcgg tcggaccgtc ggttcgctcg atcgagcccg gcgatcaggt gctgcacctg
      601 cccgagagcg gcctcgaggt cgatattcgc ggtgaggagt ttctgctgct gcgcgagcgg
      661 gacctgcacg cggtcgccag cgaccgcagc gacggcggta ccggcctcta cctctgaaga
      721 cggcggccgc caggccgccg gcggacccga taaacgcctg ccgttcttag ttgcaacctg
      781 gttgcatcta gtccgggccg gggtgtacgg tcgcttcgtg agaaccaggc tgatcgccgt
      841 ggcggccgcg cttgcctcgt ttgcggccgc gtgctcgtcc ggcggcagcc cggactcctc
      901 gggacgggtg cgcatcgtca cgaccgtctc tcccatcacc aacatcgtcg cctgggtcgg
      961 cggcgagcgc gtgtcggtga ccggcatcgt tccggaaggg accaacagcc atacgttcga
     1021 gccgcgcccc tcggacgcca ggctcgtcgc cgaggccgac gtcatcttcg tcaacggtct
     1081 cggcctcgaa gagccgacgc tcgagctcgc ggagccgaac cgcaaggaag gcaccgaaat
     1141 cgtcaagctt gcggaccgga cgatcgaccg gagcgaatgg atcttcgatt tctcgttccc
     1201 cgagagcggc ggcgatccga atccgcacct gtggaccaac ccgccgtacg ccaagcggtt
     1261 cgcgcagatc gttcgggaca cgctcacgac gctcgatccg gagggcgtcg ccctctacga
     1321 ccgcaactac ctgtcgttcg ccgagcgcat cgacgagctc gacaccgccg tgcgcgccgc
     1381 caccctcagc gtgcccgaga agaaccgcaa gctgctcacg tatcacgact cgtttccgta
     1441 cttcgcgcgc gagtacggct ggacgatcgt gggcgcggtg cagccttccg acttcagcga
     1501 gcccaccccg cgcgaggtct cgcgcctgat ccaacagatc cgcgacgagg gcgtcccggc
     1561 gatcttcggc tccgaggtct tcccctcgcc ggtgctggag cagatcgcgc gcgagtccgg
     1621 cgcgacgtac gtggccgagc tgcgcgacga cgaccttccc gggtcgaagg gtgatcccgc
     1681 gcattcgtac ttcgggctga tggtgttcga cttcaagact ttcatgaagg cgctcggcgg
     1741 cgatgtgacg gtcttcgaca ccatcgacac cgccaacccc gacgacgagt cgaacgtgac
     1801 ctaccgggaa tgagcgacct catccggctc gagaacgtga cctgctccta cggcggggag
     1861 ccggtccttt cccatgtgga cctcaccgtt catgccggcg ccttcgtcgg cgtcgttgga
     1921 ccgtcgggct cgggaaagtc caccctcctg aaggccatca cgggctcggt caagccggtt
     1981 gcgggacgga tcctgcgtcc gggcgggccc gtgaccgtcg gctatgtgcc gcaggtcgag
     2041 accgtcaact ggtacttccc cgtgaccgtg ctcgaggtgg tcctgatggc cgcgaccggc
     2101 cggcgcctgc cgtggccgag catcgcggag cgccgcctcg cgcacgacac gctcgagcgt
     2161 ctcgggctcg acgcgtacgc gtctcgtcac atccgcgaac tgtcgggcgg acagcagcag
     2221 cgggtcttca tcgcgcgggc attgttcgcg cggccgcagc tgctgctgct ggacgagccg
     2281 accagcggcg tggacgtgca cacccgccac gacgtcatgc atctgctgca cgacctgcat
     2341 cacgacggtt tggccatcct gctcaccacc cacgacctca acggggtcgc ggcgcacctg
     2401 ccggagctgg tctgtctcaa ccgggaggtc gtcgcgacgg gagcccccgg ggatgtgctt
     2461 cgccccgacg tgctcgagcg cacctacggc gcgccgatgg aggtcctgtc gcacggcggc
     2521 atgccggtgg tcgtcgaatc cgttccgccc gacgagcttc acggcaaacc cgggcatcac
     2581 gagcattccg catgagcacg cttctggagc cgctcgagct cgcattcttc cgcaacggca
     2641 tgatcgtggc caccctcgca ggcgccttgt gcgcgatggt cggcgtctac gtcgtcctgc
     2701 gcggcatgag ctacatcggg cacggcctgt cccacgcgat cttcggcggc gccgtgatct
     2761 cgttcgtggc gaaggtcaac ttcaccatcg gcgcggccgc gtggggattt ttatccgcgc
     2821 tggcgatcaa ccgggtcgcg cggcggcgaa gcatcggagc ggacgcggcg atcggcgtga
     2881 tcacgaccgc ctccttcgcg atcggcatcg cgatcatctc ccgcgcgtcg tcgctgcggc
     2941 gcaacttcga cgccgcgctg ttcggaaacg tgctcggcgt cggcccgggc gacatcgtgg
     3001 tcgtcgcggc ggtgctgacg gtcgcgaccg cgatggtgtt cgtcctgtac cggccgctgc
     3061 tgttcatcac gttcgatcct gaggtcgccg aagcctcggg cgtgcggacc ggcgcggtcg
     3121 acgctatgtt ctcgctcgtg cttgccgcta ccatcgtggc cacgatgaac gtgctcggcg
     3181 tgttgttgat cgcggccgcg ctggtgatcc cgccggtggt ggcccgcatg ctcacgaact
     3241 cgttccggcg catgctgtgg ctgtcgatcg cgatcggcgc ggccggcggc ttgatcggca
     3301 tgtacctgtc ctacttcgtg gacatggcct ccggtcccgc gatcgtcatc gtcgagtccg
     3361 ccgccttcgt tgtcgtctac gccctgtcgg gcatgcgcgg gggccggatc ctgcctgccg
     3421 gcgcggtgga ttgatgacga cgcttcccca cgaccacgac gccgcgcacg cgtccgacgt
     3481 cgcgttgatc gacctcgccg atccggccga gcgtacgcgc atccacgcgc tcatgggcgc
     3541 atgcgaggcc gccgacgggc acttgccttt cggggaccac gagctgctcg cgatccagac
     3601 gggcgcggtc gaggatcaca tcggcttcgc ggtgcacgat gaggggacgc tcgccggcta
     3661 cggccacctg tctcgcctcg gcgatgcatg gttcttcgag atcgcggtgc atcccgagat
     3721 gcggggccgg ggcatcgcga cgcgcatcct cgacgcgatc gtggcgcaca ccgccgcaca
     3781 cggtggcggc accctatcca cctgggccta ccggcaggct cccgatgtcg gcgggctcat
     3841 ccggcacgcg ggcctgcgtc cgtcacgaac cctgttgcag atgcgcctga atgcgctgcc
     3901 cgccgccgcg cccgcgctgc ccgacggtct ggtgctcgac cgcttctgcc ccggcatcga
     3961 cgacgacgag tggctcgcgc tcaacgcacg cgccttcgcc cacctgccgg atcaaggcgc
     4021 gtggacgcgc cgcgatctgc acgcgcggat ctcggcggat tggttcgacg ccgaggacgt
     4081 gctcacggtg cggcgcgacg gcacgctgat cgcgacctgc tggacgaagc tcgatccgac
     4141 cacgcgcacg cacgacgggc ggcagctcgg ggagatctac gtgatcgcgg tggatccggc
     4201 cgaggcgggt gccggcctgg ggcgcatcct tgccaccgtc gggctcgacc atctgcgcga
     4261 gcgcggcgcg caggtcggca tgctctacgc ggacaaggac aacgaggcgg ccgtcgcgat
     4321 gtacgagtcg ctcgggttcc tcgtcaacca cgaggacgtg tgcctgatca ccgacgtggc
     4381 gccgtccggc gcgacgagcg gttaggccgc ttcgcgcagg acgcgcactc gcccagcacc
     4441 gcgaagtgcg tgaaatcggc ccggaatccg tatcgatccg ccaggcgctt cgcaaagggc
     4501 gccacgaccg cgtcgtccac ctcgatgatc gcctcgcatc gctcgcagac cagatgctga
     4561 tgcagtccgc cgccgccgag gtgatacacg ctggcggtat gcccgagatg cgcgtgacac
     4621 accacgccga cctcttcgag cgaggtgagc gtgcggtaga tcgtggacgg gctgacggcg
     4681 ggcaggcgct ttcgcacgcg ggccacgatc tgctcggcgg tggcgtgtcc gtcggaggcg
     4741 gcgatcgcat cgagcgtggc ctgccgctgc ggtgtccggc gcagcccgtg cgaacgcagc
     4801 acctcgtcga ggttgtcgag ctcgtgctcg tggtcggagg gccgggcgcc tttcacgcac
     4861 cgcagtgtat ctgcaaccag ttcgcaggtg caggggctat cgctcgagca gccgcttctt
     4921 gcgcgcgtcg aactcctcgg ccgtcagagc gccacggtcg cgcaggtccg cgagctttgc
     4981 gagctcgtcg gccacggagg cggggtggtg gatcaggtcg gggccgagcc cgcggcgtcc
     5041 ctcggcggcc tcgtagatct gcttctgcac ctcgaccggg tggccgatgt cgtcgaaggt
     5101 cgtctgcccg tactcgccgg ccgactcgag cctgatgtcg cccgagcgga gaacccggtc
     5161 gagcatgctc tgggtgaccg tcacgtcgtt gacccgctcc agcgggatct cgcgggagac
     5221 cttggcgatc acgccgctgc gcgcgatgat gcgctcgttc gtcaggacga actcggtgaa
     5281 cttccatcgg gccagctgca gccccgcggc ccagatccag accatcacgg ccgcgccggc
     5341 cacggcccag cgcagcgccg ccggccgggc gggcccgatc ttgacgatca gcacggcggc
     5401 caccgtcacg atcgccatgg tggaaagcat gggctcgatg agcgctttcc agtgcggccg
     5461 tacgtcgagg atgacgtgct cgtcctcggt cagcagacgg gtcggaaacg gcatgcgcca
     5521 atggtacggc acggaccgcc gggcttcggg aatccgtgcg cgcaggaggc cgcggcgtgc
     5581 ggggcggcct cgaagtgcgc ggaagccgcg gcgtccggca ggccgcggcg ggcacggtag
     5641 actcccgcgg cgatgatcaa aggactgctc gtcgatttcg gcggcgtgct caccacgtcg
     5701 gtgatcgacg cgttcgccga gttcgcgcgc ggcgaggggc tcgatccgaa ggatctcgcc
     5761 gccgtcatgc gcgagcacat gcaggcgccc gacagcttct tccttcgcgt cgagcggggc
     5821 cacatcgaca ccgccgactt cgagatcgag ctggccggcg cgatccgggc gaagctgggc
     5881 gtcgagatcg cctcgaccgg gctgaagtcc cgcctgttcg ccggctcgcg accgaacgag
     5941 ccgatgctgg atgccctgcg gcgggcgcgt gccaacggcg tgcgcaccgc gctggtgtcg
     6001 aactcgtggg gcgtgaccga aggcggttat ccggtacatc agttcgacga gctcttcgac
     6061 gccatcgtga tcagcggcga ggtcggcctg cgaaagcccg atcccgagat ctacctgctc
     6121 gccgccgagc ggatcgcggc cgagccaccg cagtgcatct tcgtggacga cttcgccatc
     6181 aacgtcgaag gagccgtggc gaccggcatg gccggcgtgc accacgtcga aacggatgcc
     6241 acgcttcgcg agctcgaggc gttcctgagc gtcccgcttc gttagcccgc gggcacgacg
     6301 acgatctggt cgtgctcggc cgacacgtcg atcgccgcgg gcccgttcgc cgtcaccgac
     6361 ggatcaacga acacgcgcag gccgccggcg tcgacgagcg catcgccctc gtcgggctcg
     6421 tcggcgaagc cgctgcgcgc ttggcccgcc gaaatccaga cccgaacgcc gaacgcggat
     6481 gggtcgagcc gggcggcttc caggccgcgc cggagcacct cggccgcgcg tggcgtaatc
     6541 gtgacttcgt tcatggggcg cattgtccgg gcgcaagcgg gtaaactcca aaccctatgt
     6601 ctaccgacca gcagcccccc gtcctgtcgc ccgcggcgct cgaacgcttg cgggaggagc
     6661 acgagcggct caccaccgac ggcaggcgtg acatctcgga gcgcttgctt cgcgcgcgcg
     6721 agctcggcga tctgtccgaa aacgccgagt atgaccagac caagaacgat caggcgcaga
     6781 tggaagcgcg gatccgctat ctcgaatgga cgatcaagaa cgcgatcgtc caagagggcc
     6841 cgatctcgac cgactcggtc ggtccgggca tggtggtgac gctgcggtcg ctcgactcag
     6901 acgaagagcc cgagcagtac ctgctcgcgc agtcgaagga agagcgcgcc aagggcatgc
     6961 gcacgatcac gacggtctcg ccgctcggtc aggccatcgt gggccggggc aagggcgacc
     7021 agatctccta cgaggcgccg ggcggaacct tccgctacga ggtcgtcgca tacgagccgt
     7081 gggacggaag ctagacccgt ccccgcgaaa ggagcttttc gcatgaccat gtacgcgacc
     7141 ttcaagacgt ctatgggcga catcaagtgc aagctgtttc ccgatcacgc gcccaagacc
     7201 gtcgagaact tcgtcgggct ggccaccggc aagcagacct ggaaggatcc gcagaccggc
     7261 gcgcagaaga acgatccgct ctacgacgcc acgatcttcc atcgcgtcat ccccgagttc
     7321 atgatccagg gcggcgaccc gatgggaacc ggaaccggcg ggcccggata ccgcttcgag
     7381 gacgaggtcg acgacggccc gtcgttcacg cgcgccggct atctcgcgat ggcgaacgcg
     7441 ggacccaaca ccaacggctc gcagttcttc atcaccgaga tccccacgcc ctggctcacc
     7501 ggcaagcaca cgatcttcgg cgaggtcgtc gaaggcatgg aggtcgtcaa cgcgatcgcg
     7561 cgcgtcgagc gcggcgccaa cgaccggccc cgcgaggacg tggtgctgca gacggtcgag
     7621 atcagcgagt cgtgagccga accgtcacga tctcgcgccg ggcggcgtgg ctgctggtcg
     7681 gcgcggcggt ctggacgctc tacgtctgga tcacgcggat ctggatcgtc gcgggacaga
     7741 accaaagcac ctcgttcaag gttgtccact acgtccttgc cgcggtctcg atcgtgttcg
     7801 gtgccgcggt cggcgcgatc ggcgtccgcg cgctgcgtcg cgcacgagcg tgaacgccga
     7861 gctcgatgcg gccgcgcgcg cagcgcgcga ggcgggtgcg ttgctgctgg aacgctttcg
     7921 cggtcccgcg cgcggcgtgg gcacgaagtc cagcgggacc gacatggtct cggatgccga
     7981 ccgcgacgcc gagaacgtga tcaccctgac gctgcgcgaa gcgtttcccg ccgatgcgat
     8041 cttcggcgag gagaccggcg tgcacgacgg caacagcggg cgcacctgga tcgtcgatcc
     8101 gctcgacggc accaccaact tcctgtacgg aatcccgcag tggtgcgtga gcatcgcctg
     8161 cgaggacgaa cgcggcggcg ttgccggcgt ggtgtacgac ccggcgcgcg acgagctgtt
     8221 cgccgccgcg cgcggcgggg gcgcgacgct caacggctcg cccgtgcatg cctcgaccct
     8281 gtcggacgtg tcgaaggcgc tcgtggcgac cggctacggc tacgagcctt cggagcgcga
     8341 gcggtggggc ggggtcatcg cgcggctgct tccggccgtg cgcgacgttc gccgcgccgg
     8401 atcggcggcg ctagacctcg cgtggaccgc ggccggccgc gtggacgcct acgcggaggt
     8461 tccctgccgc cattgggacc gtgccgccgg agtgctgatc gcggccgagg ccgggtgcga
     8521 gatctcgatg ctcgacccga tcggtccggc cggcgacggc gtgatcgccg cgccggccgc
     8581 gctgcacgag ccgctacgcg cggcgatcga ggaggcgctc agcgcgtgag ccccgacgtt
     8641 gcgctcctct acgacgacaa ctgcggcttc tgccgccgcg cggtccgcat ggtgctcgca
     8701 tgggatcgcc acggccgcat ccgggcggtt ccgctgcagg ctcccgaggc gctcgagctg
     8761 cttcccgaca tgaccgaggc gcagcgcatg gactcctggc atctggtcgt cggcgggcgc
     8821 gtctacagcg cgggcgcggc gaccgggccg ctgatgcgtc tgctgccggc cggcgcgccg
     8881 ggcgcggtga tcgccgagac attccctcgc ggaaccgagc gcgcctaccg ctggatcgcc
     8941 gccaaccgcg agcggctcgg gcgcttcttg ccgggggccg gtggatgacg caggtcgcca
     9001 cgctgttgtt cgtcgtcctg ctggtgatgc agttctcaat gatgaagtgg gagatgatgg
     9061 cgctgcagcg gcacggacgg ccgctctggc tcgcgctgat cccgatcgtg tcgcttctgg
     9121 tgatcggcta cgacgcgatc gcgggtcgcg tgcggcgccg cggctagaga tcccgcgcgc
     9181 gctcgatcac gtcggccgcg aggtcgtagc ggccgaacgg cggcatcagg tagatgccgg
     9241 ccgcgttctc ggcgatctgc gcgctcagat ccgacgcgat cgccaatccg gtcgcctcgg
     9301 ctttgtctcc ggcggtccgc agcgcctcgc gcaccgcttc gggcacgtcg atgccgggaa
     9361 cctcgttgtg gagccattcc gcgtgccgcg cgctcaccag cggcagcaac ccgacgagca
     9421 gcgggatcgt caccggcccg tgcgcggcct cataggcggc gcggaagcgg gcgagcaccg
     9481 acaggtcgaa gatcggctgc gtcaatgcga agtccgcgcc ggactcgatc ttgcggtgaa
     9541 acaccgtgac ctcgcgttcg aggtcctcgg cgttggggtt gagagcgcac ccgaccacga
     9601 acgaggtcgc ctcgccgatc gaggcgccgg tccggtcgag cccttcgttg aagccgctct
     9661 tgaccagcga gaccaggccg ctcggcacga cgtccatggt gtcggccgcg ttcggatagt
     9721 cgccgatcgc cgtcgggtcg cccatgcacg cgaagatatt ccgcacgccc agcgcgtagg
     9781 cggcgagcag atcgccctgg acgcgcagca ggttccggcc gcgcgtgggg aagtgaagga
     9841 ccgtctcgat gccggcctcg tcctggatca ggtggcaggc ggcccacggg ctcatgcgca
     9901 tccgcgccat cggcgagtcc gcgacgtcga tcgcgtccgc gcccgcctgc cggagcgtgt
     9961 cggcgccggc gagcatgcgc gccagcgacg agccgcgcgg cggatccatc tcgacggtga
    10021 tcaccagacg gccgctggtg agcgtggcgg cgaagcgtga cgccgccggc ttgccggcaa
    10081 acggagcggg gagcggttcg gtctcttcct cggccggcag cggttcggtc gcggacgcgg
    10141 ccgcggctcg cgcgccgccg gcggcacgcg agccggccat cgcgcggatg tgtcccgcgt
    10201 ccgtgccgca gcagccgccc acgatcgcga cgcccgccgc gatcgcctgg cgcgcgaagt
    10261 cggcgaagta ctcgggtgta gcgggataga acagccggcc gctcatgcgc gaggggccgc
    10321 cggcgttggg ctgtgccgta agcgggattc cctgtgcgtg cggccgcatc gtctccagca
    10381 ggcgcagcac ctgctcgggc ccctcggagc agttcacgcc gagcgcgtcc acgccgagcg
    10441 tcgccagatg ggccgcgcac tcctccggcg tcgagcccag aagcgtccga tcgtcgcgcg
    10501 tgaacgacat ggtcgcgacg acgggcagcc ccgtctcgag cgcggctcgc acggcttcct
    10561 ctgcctcggc gaggtcggcg tgcgtctcga cgatcaggaa gtcggcaccg gcctcggcca
    10621 gcaggccggc ctgctcggcg aacgcgtcgc gcgcatcggc gggacgcacg cggccgtacg
    10681 gcgcgagccg cacgccgagc ggtcccatcg acccggcgac gtacacgccc gcgcgccgcg
    10741 cgatccccac cgcggcgacg atcagctcgc ggatgcgttc ctcgaggccg tgcgcggtga
    10801 gtttgtagcg gttcgcgccg aacgtgttcg tctcgacgaa gtcggcgccg gcgaacacga
    10861 acgagcggtg tgcctgctcg acgaccgccg ggttcgacag gttcagctcg tcgaagaacg
    10921 aatccagcgg cacgcccgcc gcgacgagcg aggttccgag cccgccgtcg ccgagcagcg
    10981 tgcctcgcgt cagtcgctca cgaaacgcct gccggtccat ggcataccag tctacggtgc
    11041 ggggaaggac ggggggtcgg gattaggctg gctccggttt cgccgtaaag ggggacgacc
    11101 gatgagcgac gaaggattcg accgcgtccg gcgcgagctg tccgagagct ttcacgtgct
    11161 caaggcccgc acgcgggtgc tcgtggactt caagaacttc atcgccaagg gcagtgcgct
    11221 cgatctcgcg atcggcgtcg tgatcggcgc ggcgttcaac agcgtcgtgc agtcgctggt
    11281 caaggacgtg ctcacgccgc tgatctcgat tcccggcgcg gccgacttca gtgacctgcg
    11341 atggtgcctg cgaaacgggc ccgaggacac gtgcgcggtc ggcctcggct acggagcttt
    11401 cgtcacggcg atgatctcgt tcctgctcac cgcggccgcg gtgttcttct tcgtcgtgcg
    11461 tccggtcaac gcgatgcgcg gctggcgccg cggcgaggcg ccgaaggcgc ccgacacacg
    11521 cgagtgcccg gaatgcctga cctcgatccc gctggcggcg acccgctgcc gggcgtgcac
    11581 ggccgtgatc gagccggcct cgtagggcgg ccggcggccc ccggatccgg gctcgggggt
    11641 cgccgctgcg ggccgggacc gggcacggta acctttggat tcctatgcct gaagacaaga
    11701 agaagcctgc caagaaagcg gccgcgaagc gcgccaccgc ccgcaagacg actgcccgca
    11761 agaccggtgc ctccaagggc gcgagcggcc ggggggcgag cgcgcgaagc aaggctccgg
    11821 ccaagccagc gactcgcaag accccggccc gcaagcccgc gcccggacgc aagcccgctg
    11881 ccgccgagga atccgagctc gagcagtcga gcgtggatcg cgacgaggac gacgcgccgc
    11941 tggagcagcg cacctccgca ccgatcgtga tcggcgccgg cgcatcccgg ccgggtgagc
    12001 cggttgtcgc ggcctcgagc gagaccgtcg cgatcgccga cgccgccacc ggcgaagcgg
    12061 tcgcggtgac cgaccaagcc cccgacgagc cgcccgtccc cgagcggtat ccggaagaca
    12121 ttccggtcat ggtcgtgtcc gcgctccttg ccgtgtcggt gttcctgccc tggtacaagc
    12181 tgaccagcgc gtttcggcag acgaccgtga ccgcctcggg ctgggagacc ggaacctggg
    12241 gcccgatgat cttcttcctg ggcctggcat cccttgcgat cgccgtgttc cgtcgcgccg
    12301 gcgtgcgcgt cgggctgccg gtggccgatt cccacctgca cgaagggatc ggttgggccg
    12361 ccatcatcgg cgcggtcatc aagctgcgcg ccgtcccgaa ggagcagggc attcagatgg
    12421 gttggaactg gggcatcatc gtggcgatcg tcgccgcgtt cgcgctcgcg ttcctcgccg
    12481 gccggatgtc ggggtcgatg acgttcacca ccatccccgg ctggttccgc ggacgggccg
    12541 gcaagatcgg gctcgccgtg ctcctgctcg cgatcgcggg ttcggcgttg ttcggcatca
    12601 cgcagaacgt gagcccgtac gcgctggcga ccggccgtcc gacgacggac ggcggcggcg
    12661 acggcggtgt cgccagcaac gtgctgcgcg acgagttccc caagtgcgcc gaaggcttcc
    12721 ctcgtccggc cgacgcgaag ccgcgcgaag gcgttgagca aaagggccaa agcccgtgcg
    12781 tgttcacctt ctcgaccgcg gagacgcccg acgagctgct gaagttctat acgagcgagc
    12841 tcaagaagga cggctggtcg gtgcaggcgc tgccgtcgcg gggcgaaggc atcggtcacg
    12901 ccatgacgtt cagctcgccc aagtgcggct cgatgtcgat gcagcgcgcg caaggcgcga
    12961 gcgagtcgaa cgtgatcgtc gccgtcggcg ggccgtgccc gaccttccag cccggcggat
    13021 ccggatccac gccgacgccg agcccgagct gacgaacgat caaggaggag tcatggccgc
    13081 ccacgggaac gtgccgctga ccgaggccga cccgacggtc gccgggctga tccgcgacga
    13141 ggagcagcgc gagcgcagcg tcgtgcggct gatcccgtcc gagaactacg tctcgcgcgc
    13201 ggtgctcgaa gcgacgggat cggtgctgac caacaagtac agcgaaggct atccgggcaa
    13261 gcgctactac gaaggccagc agttcatcga cccgctcgag acgctcgcga tcgaacgcgc
    13321 caccgggctg ttcggcgccg accacgcgaa cgtccagccg tactcgggct cgcccgcgaa
    13381 cctcgccgtg tacttcgcgt tcctgcagcc gggtgagacg gtgatgggca tggcgctgcc
    13441 gcacggcggg catctcacgc acggctgggg cgtgtcgatc accggcaagt acttccgtcc
    13501 cgtccagtac gaggtgcgcc gcgaggacgg ccgcatcgac tacgaccagg tgcgggacct
    13561 cgccaaggcc gagcgcccca aactgctgtt cgccggcgga accgcgatcc cgcgcacgat
    13621 cgacttcgcg gccttcggcg agatcgcccg cgaggtcggc gcggtgttcg tcgccgacat
    13681 cgcccacatc gcgggcttgg tcgcaggcga cgcgcatccg tcgccgctgc cgcacgccga
    13741 tgtcgtcacc acgaccacgc acaagacgct gcgcgggccg cgcggcgcga tgatcctgtg
    13801 caaacaggag cacgccgccg cgatcaacaa ggcggtgttc cccgggctgc aaggcggccc
    13861 tcataaccac accaccgccg ggatcgccgt cgcgctgcac gaggcgtcgc agccgccgtt
    13921 caaggactac gcggcgcaaa tcgtcgcgaa cgccaaggcg ctcgcgacgg cgctgaccga
    13981 gcgcggctac gatctggtct cgggcggcac ggacaaccac ctgatcctga tcgacctgac
    14041 gaggaacgac gtgcccggca agatcgcggc taaggcgctc gacaaggccg gcctggaatg
    14101 caactacaac acggtgccgt tcgatccgcg caagccgttc gatccgtccg ggatccggat
    14161 cggcacgccg gccgtcacct cgcgcgggat gggcgaggca gagatgacgc agatcgcagc
    14221 ctggatggac gccgcgatcg ccgccgccga cgacgacgcc gcgctcgcga agatcgccgg
    14281 cgaggtcgcg gccctgtgcg agcagttccc cgccccgggt ctcgcgatcg gcgattgacc
    14341 ggaaccgggg ccggtgccga gaccggatgg tgaagcggcg atgggttcat gcggccgcga
    14401 tcgcggtcgt ggccgccatc ttcgtcatcc cgctcgtggt gctcgtcacc ggatcgctgc
    14461 gcttgcccgg gcaggcgccg ccgcgcacgc tcgaggtgat tcccgacccg gtcacgctcg
    14521 acgcctggcg tgacgcgttc gacttctcgc cgcttgcgcg ttcgatggtc aactcgctga
    14581 tcgtcgcggc cgtcttcgtc ccgctcgcgg tgcttgcctc gtcgtgggcg ggctttgcga
    14641 tcgcgctggc gcgcgagcgg ctccgcggac ggctgatcgt gctcgcgctg ctgctgatga
    14701 tggtcccggc gacggcgttg tggctgacca ggttcgtgat cttcaaggcc ttcggcatgg
    14761 tggacacgtt ctggccgctg gtgcttccgg cgctgctggg cggctcgccg ctcgccgtgc
    14821 tcctgtacgc ctacgcgtac ggtcgcatcc ccggcgagct ctacgacgcg tcgcggctcg
    14881 aagggctgag ccccgtacgc atctggtggc gcgttggcat gccgctcgtg cgggccacga
    14941 ccgtggcggt cgcgatgctc gccttcctga ccacgtgggc gaacttcatc gacccgctgc
    15001 tctacctgaa ctccgaggat cgctttaccg caccgctgac gctgcgctac ctcgagcagc
    15061 tcggtccgac gaactggccg gtactcttgg cgggtgccgc cgccgtgacc gcgccggtcg
    15121 tggttgtgtt cctgctggcg cagcgctttt tcctgcaaga agaaagagga gttggatggc
    15181 tcggacgatg aagctcgtcg cgctcgtggt cgcaacgctg acggcgcttg cggcgtgcgg
    15241 aggcgaggac tcgcgggcgg gttccacgaa gcaagtttcc ttccagatct tcggcgatgc
    15301 cgccgagatc aaggcctatc agtccctcat cgacgccttc caggccaaga ccccgggcgt
    15361 caaggtcaag ctcgtgcccg tgccgagtca gaatgatcat tccgccaagc tgtcggcggg
    15421 gttcgcgggc ggcagcccgc ccgacctgtt cctgatcaac ttccgccgct tcggccagta
    15481 cgccgcacgc ggcgcactcg agccggtcga gccgatgctc gagtcgagca cgaccatgac
    15541 ggagtccgac ttctatccgc aggcgatcga cgcgttccgc ttcggcggca cgctgacctg
    15601 catgccgcag aacatctcca gccaggtcgt ctattacaac aaggagctgt tcaagcgctt
    15661 caacgtcgcc gagccgaagg ccggctggac ctggacggac tttctttcga ccgccaaggc
    15721 gctgaccaag gacaccgacg gggacggcac caccgatatc tacggcctcg gcgtggatcc
    15781 gacgctggtc ttcctcgggc cgtgggtctg gcaggcgggc gcacaggtcg tggacgacac
    15841 cgacaatccg tcgcagaccg tgatgctcga cgacaaggcg ctcgaggcgt tgaagttctt
    15901 catcgagctt cgccgtatcc acaaggtcgt tcccgaccac gccgaggccg agagcgaggg
    15961 cgcggaggcg cgcttcggca acggccgctc cgccatgctt atcgacagcc gccgcggcac
    16021 gaccgcgctt cggcagcaaa ccggcctcga ctgggacgtg gtgcaaatgc cgaagttccg
    16081 cgaagaggcc acgatgctgc acagcgacgc ctactgcatg tcgaaggcat ccaagagcaa
    16141 ggacgcggcg tggaagttcg tcgagttcgc gttcggcgag gaaggcgcgg cgatcatcgc
    16201 caagaccggc cgtaccgtgc cgtcgctcaa gaaggttgcc gagagcccgg cgttcctgga
    16261 cccaacgcag aagcccgcca acgcacacgc ctggctcgac gcgatcccca cggtccggcg
    16321 tctgccgaac atctcgacct ggaacgagat cgagtccaag gccaacgtcg tgatcgagga
    16381 atggttctac gggcaggagc ggatcgaggc gctgggcatc gagatcgacc tcgcgacgcg
    16441 cgagctgttc gcggaggcca aggccgctcc gtcgccggga tcgtcctagg aggcagatgg
    16501 ccgggctctc gcttcgcggc gtcaccaagt cctacggcga gcatccggcc gtggaggatc
    16561 taacgatcga cgtcgccgac ggcgagctgt tcgtgctgct cggcccgtcc ggttgcggca
    16621 agtccaccac ccttcggctg atcgcgggtc tggagcgcgc gaacaccggc acgatccgta
    16681 tc
//
LOCUS       DATLFO010000096         8854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_43704, whole genome shotgun sequence.
ACCESSION   DATLFO010000096 DATLFO010000000
VERSION     DATLFO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43704"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VM841_03260"
     CDS             <1..494
                     /locus_tag="VM841_03260"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HVL89235.1"
                     /translation="REQRLTQIRELIDGLAPYAGLGAVEGPGIRVELRDSTLRDSPTG
                     DPNDLVIHEEDLQAVVNALWAGGAEAVTINGERVTTLTAIRCVGNTLLLHGSVYSPPY
                     KIAAIGSHAKLTEALNADDSVERLRIVAEKFKLGFTVTKSDRVSAPAFRGITALRFAK
                     LAG"
     gene            491..1201
                     /locus_tag="VM841_03265"
     CDS             491..1201
                     /locus_tag="VM841_03265"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HVL89236.1"
                     /translation="MSTLEPSPHAVADGARPASPGRARKPGRGYRIAGWTLIALGAVI
                     LEFALYELVGTSMVTKNHQNALREEFEQLIDDPVVDPAPAESPAADPPPRPIDSRAIA
                     LLKIPKVDVDVIVVEGVNLSQLSRGPGRYPSTAKFGAQGSTAIAGHRTGWGSPFLNLD
                     KLQSGDEITVESVGGKLYRYRVTKRVIVGPEAVWVLRGNPDSEAASQLTLTTCDPKFT
                     SRNRMIVWADLISSETVA"
     gene            1198..1365
                     /locus_tag="VM841_03270"
     CDS             1198..1365
                     /locus_tag="VM841_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89237.1"
                     /translation="MSAEGMRMERRRAKKRRTTRVLAIAAAVLAVAVILWIWVFPWVD
                     RTFVNRPQIGL"
     gene            complement(1362..3725)
                     /locus_tag="VM841_03275"
     CDS             complement(1362..3725)
                     /locus_tag="VM841_03275"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVL89238.1"
                     /translation="MEQWVEALREHAASALRPTEGTLTVDGPDGAIEIVTDTWGVPHV
                     YASSRDDALFAQGWLHAAERLWQIEFTVRAATGRLAEIVSIAGLSLDKFFRTLGIGRL
                     AREWARSADEVTREIAASYHAGFVAAATALPRPVEFEFLDLDPTIPESLEDTIASSFA
                     FVGLMGFTLSTNWPFELLRAELARHLGIERARELTPFVGAESPVAVPSSPSFPGTAQA
                     LRAIAHDAGGVHGIGSNNWVVAGSKTNTGTPILCNDPHLLVQMPAIWMEMHLDAPGLR
                     VAGLTLPGIPGIVIGHNERIAWGFTNTGADVEDLYLERLDETRTRYEYDGRWRDLATI
                     EEKIFVRGEADPLLHVVHETHHGPLLTGWIAAGSQPVVLEDRIRDPLALRWILRETPP
                     SMAGLIGMNTASNWAEFRAAAALWPTAGQNMVYADVDGNIGYQFTGTIPIRGKGAGSA
                     PLPGWDPAYEWTGTIPFDDLPSSFNPERGFIATANHRMVDLDYPHYLTNDWELGHRIR
                     RITALLTERETLGIDDMKRIQADTFSGVAADLVPLFLCADVSGDREAEALASVQAWDL
                     RLERESVGGAIFNAWFARVAELLLADKLGAELWNEFYPRKGWTTNWIYESVRDIIQNP
                     QAFWVGGGGDNLAARDALLGRALSSAVADLTGRLGEDMSGWRWGRLHQVHFAHVLAVP
                     IPSLHGLLSAGPFEAAGGDDTINRGVIFPGEGFVDGAIPSARLIVDLGDFDRSEAVIT
                     TGVSGNPASPHYRDQAELWARGDYHPMPFTREAVEKSAKARLSIEPA"
     gene            complement(3775..4728)
                     /locus_tag="VM841_03280"
     CDS             complement(3775..4728)
                     /locus_tag="VM841_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HVL89239.1"
                     /translation="MRRPAFIVLAVAFAFANASVTSTASTESPDALTSRITHDLPGLR
                     AHGIAMIAPEADGAVVLHVTGDVNTARRDLTARYGERVRVVPGQAFRPLAGPPCLAQR
                     THCGPVMRGGLELIGDTLVTCTSGVEARLNRNGEIGVITAGHCFRNGQVVTHGGMLLG
                     PAGPSTFSGGVDGAFVTHSLGAPTFLPSNWVYYSGSDREHAITAVQSMAAEKVGQSVC
                     RTGRTTSLQCGTITSVNATIVIGAVTLTNQRVVNVCALPGDSGGPFLSGGTFRGIVSA
                     GNYVGSGSSASCHGTPFTTYSPAERIQSVLGVTVLTSSPLF"
     gene            4812..5507
                     /locus_tag="VM841_03285"
     CDS             4812..5507
                     /locus_tag="VM841_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HVL89240.1"
                     /translation="MLIDLHIHASPASRDARDDVAAIARAVEPLGVDAIALTDHGPGC
                     DYAAAAAALAERGIGLVPGRELNTPLGHVLVLSPDVVWLRDLPERSALPVPGGTREPF
                     ALVWAHPAGWRIAGAMVAPDPSKGAEHVHAVEILNGERLWQLDGVGIAETLADRLGAG
                     TTGGSDAHRADAAGRCLTLADGARDAREVVEAVRAKATRPVLGSGWAAAHSHVYERPD
                     LRAYAWPGNAPAR"
     gene            5545..7224
                     /locus_tag="VM841_03290"
     CDS             5545..7224
                     /locus_tag="VM841_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL89241.1"
                     /translation="MEAIWLKNYPPGIPHSIEYPLVPVTAMLDEAASRYPSAPAMDFQ
                     GARTTYSQLKQTVDRFASALASLGVSKGTRVGLILPNVPQNVIAYYAAMRLGAIVVEN
                     NPMYTERELAHQLDDAGVEVLVTLDLFYDKVKALRPELPKLREVVVTDVFDGLQGLKA
                     KLGRLLKGKELSKPVPASEPVKQFRDLVKNGSPNIAQANINPKEDLALLQYTGGTTGL
                     SKGVMLTHYNLVANAHMLKAWLPGTQPGREVIVCALPIFHSYGQTVCMNVGILLAGML
                     VLVPNPRDLDGLLKTIDRTKPTLFPGVPTLYINLLAHPEIGKYDMKSIRACVSGAAPL
                     PIDVQEEWERVTGGKIVEGYGLSETSPSTHANPIEGKRKIGTIGLPMPDTRCKLVDVN
                     DPTKEIEAPGPGELCIKGPQVMQGYWNRPEETAETIRGGWLHTGDIAEVDDEGYFKIV
                     DRKKEMIIVGGFNVYPRDVEEVLFQHPKIQEAAVAGIPSKKSGEMVKAYIVLKPGETA
                     TEDEIEAFCRERLTAYKVPKAFEFRAELPKTMIGKVLRRVLVEEEKAKAGG"
     gene            complement(7251..8300)
                     /locus_tag="VM841_03295"
     CDS             complement(7251..8300)
                     /locus_tag="VM841_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89242.1"
                     /translation="MKAQSRYVRTTVVGTVAAVAVLALVIAVGAPMLRGDLAEVVAPP
                     TPAKVVQTVDLGEQNADGDIVWDGSNFVVGIGGGRSALAGALIDPDASIPRKIDGLSV
                     HAEAVVADSAGGRSYFFGFAGIKVVEAGTIVATFPGYYASPAFDSSTGRLYALETMTG
                     VGQSSSARLVLINTQTGTVERRSEPVEEQGIFTLVASGGKLVLADRGPRPFRVFDGQT
                     LEETGFAPTNGGPEGGPLDLAVSPSGNRAVVLIDEETPVLRLYDVAKPVAATDITLGV
                     ADATGLAYDAKGERLFTVSGENLLVIDAASGKVTQTLPLFRGTIVGASLAYDGVNNRL
                     GVLAKDAGRVLIVKI"
     gene            complement(8377..>8854)
                     /locus_tag="VM841_03300"
     CDS             complement(8377..>8854)
                     /locus_tag="VM841_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL89243.1"
                     /translation="SMAGWFASQYDDLRRFGFVLSGDWSIADDLVQEAFVRICSRPRR
                     VEREGFPAYARRTMVNLWRTALVRIRLERRHATDGDRLTAPDSGSVAGNDQVWQAVMA
                     LSPQQRAVVALRYYEDMSEADIAAVLGVSLGAVKKQLNRAKEKMRATLGEGERHGS"
BASE COUNT         1508 a         3017 c         2978 g         1351 t
ORIGIN      
        1 ggcgcgagca gcggctcaca cagatccgcg agctcatcga cggactggcc ccctacgcgg
       61 ggctcggtgc cgtcgaaggg cccggcatcc gcgtcgagct tcgcgactcg acgctccgcg
      121 actcgcccac cggcgatccc aacgatctcg tcatccacga agaggacctg caggccgtcg
      181 tgaacgcgtt gtgggcgggc ggcgcggagg cggtcacgat caacggcgag cgcgtgacca
      241 ccttgacggc gatccgctgc gtcggcaaca cgctgctgct gcacggctcc gtgtactcgc
      301 cgccgtacaa gatcgcggcc atcggatcgc atgcgaagct gaccgaagcg ctcaacgccg
      361 atgacagcgt cgagcggttg cgcatcgtcg ccgagaagtt caagctcgga ttcaccgtga
      421 cgaagtccga tcgagtttcc gctcccgcgt tccggggcat caccgcgttg cgtttcgcca
      481 agctagccgg atgagcactc tcgagccgtc cccgcacgcc gtcgccgacg gcgcccggcc
      541 cgcatcgccc ggacgggcac gaaagcccgg acgcggctat cgcatcgcgg gctggacgct
      601 gatcgcgctc ggcgcggtga tcctggagtt cgcgctctac gagctcgtgg gcacatcgat
      661 ggtgaccaag aatcatcaaa atgcactgcg cgaggagttc gagcaattga tcgacgaccc
      721 ggtcgtcgat cccgcgcccg ccgagtcgcc ggcggccgac ccgccgcccc gccccatcga
      781 ctcgcgcgcg atcgcgctac tgaagatccc caaggtcgac gtggacgtga tcgtcgtcga
      841 aggcgtcaac ctgtcgcagt tgagccgcgg acccggccgg tatccgtcga ccgcgaagtt
      901 cggcgcgcag ggatcgaccg cgatcgcggg acaccggacc ggttggggat cgccgtttct
      961 gaacctcgac aaactgcaaa gcggcgacga gataaccgtc gagtccgtgg gcgggaagct
     1021 ctaccgctac cgcgtcacca aacgcgtgat cgtcgggccg gaagccgtct gggtgctccg
     1081 aggcaatccg gattcggagg ccgcgtcgca gctgacgcta accacctgcg atccgaagtt
     1141 cacgtcgcga aaccggatga tcgtctgggc cgacctgatc tcgtcggaga ccgtggcatg
     1201 agcgcggagg ggatgcggat ggagcgccgt cgcgcgaaaa agcgccgcac gacccgcgtg
     1261 ctcgccatcg cggcggcggt cctggcggtc gccgtcatcc tgtggatctg ggtctttccc
     1321 tgggtggacc ggaccttcgt gaaccgcccg cagatcggcc tttaggccgg ctcgatcgac
     1381 aagcgcgcct tcgcggactt ctcgaccgcc tcgcgggtga agggcatcgg gtgatagtcg
     1441 ccgcgggccc agagctcggc ttgatcgcgg tagtggggac tggcgggatt gccgctcacg
     1501 cccgtggtga tgacggcttc gctgcggtcg aagtcgccga ggtcgacgat caggcgcgcg
     1561 ctcgggatcg cgccgtcgac gaacccttcg ccggggaaga tgacgccacg attgatcgtg
     1621 tcgtctcccc ccgcggcctc gaacggcccg gccgacagca ggccgtgcag cgacgggatc
     1681 ggcacggcga gcacgtgagc gaagtgaacc tgatgcagcc ggccccagcg ccagccgctc
     1741 atgtcctcac ccagccgccc ggtcagatcg gcgaccgccg acgacagcgc gcggccgagc
     1801 agcgcgtcgc gggccgcgag gttgtcgccg ccgccgccga cccagaacgc ctgcgggttc
     1861 tggatgatgt cgcgcaccga ctcgtagatc cagttcgtcg tccatccctt gcgagggtag
     1921 aactcgttcc atagctcggc gcccagcttg tccgccagca gcagctcggc gacacgcgcg
     1981 aaccaggcat tgaagatcgc gccgccgacg gactcgcgtt cgagccgcag atcccatgcc
     2041 tgcaccgatg caagggcctc ggcctcgcga tcgccggaga catcggcaca caggaagagc
     2101 ggtaccagat cggcggcgac gccgctaaag gtgtcggcct ggatgcgttt catatcgtcg
     2161 atgccgagcg tctcgcgttc ggtcagcagc gcggtgatgc ggcggatgcg atggccgagc
     2221 tcccagtcgt tggtgaggta gtgcgggtag tcgaggtcca ccatccggtg attggccgtt
     2281 gcgatgaagc cacgctcggg attgaagctg gagggaagat cgtcgaacgg gatcgtcccg
     2341 gtccattcgt aggccggatc ccagcccggc agcggcgcgg agccggcgcc cttgccgcga
     2401 atcggaatcg tgccggtgaa ctgatagccg atgttgccgt ccacgtcggc gtagaccatg
     2461 ttctgcccgg cggtgggcca caaggctgcg gcggcacgga actcggccca gttcgaggcc
     2521 gtgttcattc cgatcaaccc ggccatcgac ggcggcgtct cgcgcaggat ccagcgcagc
     2581 gcgagcggat cgcggatgcg gtcctcgagc acgaccggct gtgagccggc ggcgatccat
     2641 ccggtcagca gcggcccgtg atgggtctcg tgcaccacat ggagaagcgg atcggcctca
     2701 ccacggacga agatcttctc ctcgatggtg gcaaggtcac gccatcttcc gtcgtattcg
     2761 tagcgcgtgc gggtctcgtc gagccgctcc agatacaggt cttcgacgtc ggcgccggtg
     2821 ttggtgaatc cccacgcgat gcgctcgttg tggccgatga cgatcccggg gatgcccggc
     2881 agcgtcaggc ccgccacgcg caaaccgggc gcgtccaggt gcatctccat ccagatcgcg
     2941 ggcatctgta ccagcaggtg cgggtcgttg cacaggatcg gcgtgccggt gttggtcttg
     3001 gatccggcca cgacccagtt gttcgagccg atgccgtgca ctccgcccgc atcgtgcgcg
     3061 atcgcccgca gcgcctgcgc ggtgccggga aacgacgggg acgaaggcac cgcgaccggc
     3121 gattcggcgc cgacgaacgg cgtgagctcg cgcgcgcgtt cgatgcccag atgccgcgca
     3181 agctcggctc ggagaagctc gaacggccag ttggtggaca gcgtgaagcc catcaggccg
     3241 acgaacgcga atgaggaagc gatggtgtcc tcgagcgatt cgggaatcgt cgggtcgagg
     3301 tcgaggaact cgaattcgac cgggcgcggc agcgcggtgg cggccgcgac gaagccggcg
     3361 tggtaggacg cggcgatctc gcgcgtgacc tcgtcggcag agcgggccca ctcgcgggcg
     3421 agacggccga tgccgagcgt ccggaagaac ttgtcgagcg agagcccggc gatggagacg
     3481 atctcggcga ggcgcccggt cgcggcgcgg accgtgaact cgatctgcca gagccgctcg
     3541 gccgcgtgca gccatccttg agcaaacagg gcatcgtcgc gcgaggacgc gtacacgtgt
     3601 ggcacgcccc aggtgtcggt cacgatctcg atggccccgt cgggcccgtc gaccgtgagg
     3661 gttccctccg tcggacgcag cgcgctcgcc gcgtgctcgc ggagcgcttc gacccattgt
     3721 tccatcgccg gcctccctgt ccgggcgacg atactgcggc aatcgagcct ttggctagaa
     3781 aagcggcgaa gaagtgagca ccgtcacgcc gaggaccgac tgaatgcgct cggccgggct
     3841 gtaggtggtg aacggcgtgc cgtgacagct cgccgaggag ccgctgccga cgtagttgcc
     3901 ggcggagacg atcccgcgga acgttccgcc gctcaggaac ggcccgccgc tgtcgcccgg
     3961 cagcgcgcac acgttcacga cccgctgatt ggtcagcgta acggcaccga tgacgatcgt
     4021 cgcgttgacc gacgtgatgg ttccgcattg aagactggtg gtgcggcccg tgcggcacac
     4081 ggactggccg accttctcgg ccgccatgga ctgcaccgcg gtgatggcgt gctcgcggtc
     4141 ggaacccgag tagtagaccc agttcgaagg aaggaaggtc ggcgcgccga gcgagtgagt
     4201 cacgaaggca ccatcgaccc cacccgagaa cgtcgacggc cccgccgggc ccagcagcat
     4261 gccgccgtgc gtgacgacct ggccgttacg gaagcagtgt ccggccgtga tcacgccgat
     4321 ctcgccgttg cggttcagcc gcgcctcgac tccgctggtg cacgtcacga gcgtatcgcc
     4381 gatcagttcg agcccgccgc gcatgaccgg cccgcagtgg gttcgctgcg cgaggcaggg
     4441 cggaccggcc agcggccgga acgcctgtcc cgggaccacg cggacccttt cgccgtagcg
     4501 agcggtcaga tcgcgccgcg ccgtattcac gtcaccggtc acatgcaaca ccaccgcgcc
     4561 atccgcctcc ggcgcgatca tggcgatccc gtgggctcgc aggccgggca gatcgtgcgt
     4621 gatccggctc gtgagcgcat ccggcgattc ggtcgacgca gtgctcgtaa cggacgcgtt
     4681 ggcgaacgcg aacgccactg caagcacgat gaatgcgggc cgtctcataa tcgtcgtctc
     4741 tctggaagcg gtaccgtatc gcaacacttc gccctattgg tcccgatgtc ctttccaccc
     4801 ggcccgaatc catgctcatc gacctccaca ttcacgcctc gccggcctcg cgcgatgccc
     4861 gtgacgacgt cgcggccatc gcccgggcgg tcgagccgct cggcgtggac gcgatcgccc
     4921 tgactgatca cggtccgggc tgcgactacg ccgcggcggc ggcggcgctc gccgagcgcg
     4981 gcatcgggct ggtgccgggc cgggagctca acaccccgct cggacatgtt ctggtcctgt
     5041 cccccgacgt cgtctggctg cgggatctgc cggagcggtc ggcgctgccc gttcccggcg
     5101 gaacgcgcga gccgttcgcg ctggtgtggg cgcatccggc gggatggcgg atcgcgggcg
     5161 cgatggtggc gcccgatccg tcgaagggag ccgagcacgt gcatgccgtc gagatcctca
     5221 acggcgagcg gctctggcag ctcgacggcg tgggcatcgc ggagacgctc gccgatcggc
     5281 tcggggccgg cacgaccggc ggatcggacg cgcatcgcgc ggacgccgcg ggccgctgcc
     5341 tcacgctcgc cgacggcgcg cgtgacgcgc gcgaggtggt cgaggcggtg cgagccaagg
     5401 ccacacggcc ggtgctcggc agcggatggg ccgccgcgca ctcgcacgtc tacgagcggc
     5461 ccgacctgcg cgcgtacgca tggccgggga acgccccggc gcgatagatt gcccacggtg
     5521 gcgtcggcgg acaggagaga tcgaatggaa gcgatctggc tcaagaacta tcccccgggg
     5581 attccccact cgatcgagta cccgctggtg cccgtcaccg cgatgctcga cgaagccgcc
     5641 tcgcgctatc cgagcgcgcc cgcgatggac ttccagggcg cgcgcacgac gtactcacag
     5701 ctcaagcaga ccgtggaccg gttcgcgtcg gccttggcat ccctcggcgt gtcgaagggc
     5761 acgcgtgtcg ggctgatcct gccgaacgtg ccgcagaacg tcatcgccta ctacgccgcg
     5821 atgcgcctgg gtgcgatcgt ggtcgagaac aacccgatgt acaccgagcg cgagctcgcg
     5881 caccagctcg acgacgcagg cgtcgaggtg ctggtcacgc tcgacctgtt ctacgacaag
     5941 gtcaaggcgc tccgccctga gctgccgaag ctgcgcgagg tcgtcgtcac cgacgtcttc
     6001 gacgggctgc agggcctcaa ggccaagctc ggccggctgc tcaagggcaa ggagctttcg
     6061 aagccggttc ccgcgagcga gccggtcaag cagttccggg acctggtcaa gaacggaagc
     6121 cccaacatcg cgcaggccaa catcaacccg aaggaagacc tggcgctgct gcagtacacc
     6181 ggcggcacca cggggctgtc caagggcgtg atgctcacgc actacaacct cgtcgcaaac
     6241 gcgcacatgc tcaaagcgtg gctgcccggc acgcagcccg gcagggaagt catcgtgtgc
     6301 gccctgccca tcttccactc ctacggacag acggtctgca tgaacgtggg catcctgctc
     6361 gccggcatgc tggtcctcgt gccgaacccg cgtgatctgg atgggctgct caagacgatc
     6421 gaccgcacga agccgacgct gttcccgggc gttcccacgc tctacatcaa cctgctcgcg
     6481 catcccgaga tcggcaagta cgacatgaag tcgatccgcg cgtgcgtgtc gggcgcggca
     6541 ccgctgccga tcgacgtgca ggaggagtgg gagcgcgtca ccggcggcaa gatcgtcgaa
     6601 ggctacggcc tgtcggaaac ctcgccgtcc acgcacgcga acccgatcga gggcaagcgc
     6661 aagatcggca cgatcggcct gcccatgccc gatacccggt gcaagctcgt ggacgtcaac
     6721 gaccccacga aggagatcga ggcgcccggt ccgggcgagc tgtgcatcaa ggggccgcag
     6781 gtgatgcagg gctactggaa ccggcccgaa gagaccgccg agacgatccg cggcggctgg
     6841 ctgcacaccg gcgacatcgc cgaggtggac gacgagggct acttcaagat cgtggaccgc
     6901 aagaaggaga tgatcatcgt cggcggtttc aacgtctatc cgcgcgacgt cgaagaggtc
     6961 ctgttccagc atccgaagat ccaggaggcc gccgtcgccg gcattcccag caagaagtcc
     7021 ggcgagatgg tcaaggccta catcgtcctg aagcccggcg agaccgccac cgaggacgag
     7081 atcgaggcgt tctgccgcga gcggcttacc gcctacaagg tccccaaggc cttcgagttc
     7141 cgcgccgagc ttcccaagac gatgatcggc aaggtcctgc gccgcgtgct cgtcgaagag
     7201 gaaaaggcaa aggccggggg atgagccccc ggcctttgcg tcgtagcgcc ttagatcttg
     7261 acgatcaaca ccctgccggc gtccttggcc agaacaccga gtcggttgtt cacgccgtcg
     7321 taggccagcg aggcaccgac gatggtgcct cggaacagcg gcagcgtctg agtcaccttg
     7381 ccgctggcgg catcgatcac cagcaggttt tcgccggaga cggtaaacaa gcgctcgccc
     7441 ttggcgtcat aggcaaggcc ggtggcgtct gccacaccca aggtgatgtc cgtcgcggcg
     7501 accggcttgg ccacgtcgta cagacgaagc accggggtct cttcatcgat cagaaccaca
     7561 gcgcgattcc cggacggcga gacggccagg tccagcgggc ctccctccgg gccaccattc
     7621 gtgggagcga agccggtctc ctcgagggtc tggccgtcga agacacggaa cgggcgcggc
     7681 ccgcggtccg ccagtaccaa cttgccaccc gatgccacta gcgtgaagat gccctgctct
     7741 tcgaccggct cggaccggcg ttcgaccgtg cccgtctgcg tgttgatcaa aaccagacgg
     7801 gcgctcgacg actgaccgac cccggtcatc gtttccaacg catacagccg gccggtagac
     7861 gaatcaaagg ccggagaggc atagtacccg gggaacgtgg cgacgatcgt gccggcctcg
     7921 acgaccttga tgcctgcgaa gccgaagaag tacgagcgtc ctcccgcgga atcggccacg
     7981 actgcctcgg catgaacgga cagcccgtcg atcttgcgcg ggatcgaggc gtccgggtcg
     8041 atcagcgcgc cggccagcgc cgaacgtccc ccgccgattc cgacgacgaa gttcgatcca
     8101 tcccacacga tgtccccgtc ggcgttctgc tcacccaaat cgacggtctg cacgaccttc
     8161 gcgggtgtgg gaggcgcgac gacttcggcc aaatccccgc gaagcatcgg cgcacccaca
     8221 gcgatgacca gcgcaaggac ggcaaccgca gcgacagttc cgacaacggt ggtcctgacg
     8281 taccggctct gtgctttcat tgcgatctcc ccccacgtct tttctcccgg cggcggatcc
     8341 gccagccggc gctgcatcgt ctctctcaac tcgcgatcaa gatccatggc gctcaccctc
     8401 tccgagagtc gcgcgcatct tttccttcgc gcgattgagc tgtttcttca cggctccgag
     8461 cgacacgccc agcacggcgg cgatgtcggc ttcggacatg tcttcgtagt agcgaagcgc
     8521 gacgacggcg cgctgttgcg gcgacaacgc catgaccgcc tgccacacct gatcgtttcc
     8581 ggccacggag cccgaatccg gcgcggtcag ccggtccccg tccgtcgcgt gccggcgctc
     8641 cagccgaatc cggaccagcg cggttcgcca taggttgacc atggtccgcc gcgcataggc
     8701 gggaaagcct tcccgttcga ctcggcgggg ccggctgcag atccggacga acgcctcttg
     8761 gaccagatcg tcggcgatgc tccagtcacc cgacaagacg aacccgaacc ggcggaggtc
     8821 gtcgtactgg gacgcgaacc agccggccat ggaa
//
LOCUS       DATLFO010000097         7104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_81978, whole genome shotgun sequence.
ACCESSION   DATLFO010000097 DATLFO010000000
VERSION     DATLFO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81978"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1584
                     /locus_tag="VM841_03305"
     CDS             61..1584
                     /locus_tag="VM841_03305"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL89244.1"
                     /translation="MNVASLAERNVEKFGTYTQLWFEGREFTNVEMLEKAARFATALK
                     DAGVKEGDRVGVMLPNLPEVGIAYGALLRMGAIVMPMVFLLAVPEIRHIIEDSEATII
                     ITSPEFRPNVAAACEGIANPPRTIVVGDTTPEGCLSYAQMVESPAEPHPVVERGADDL
                     AVIMYTSGTTGHPKGVMLTHGNLLFNAENSAKAVDLRDGDVSVQGLPQAHLFGLASSI
                     TGSLFKVKGILLRWFTAEAFFEAVNTHRANSSGVVPTMLTYMLSHPQFDDVDWSSMRW
                     VVCGAAPLPVEVAAEFEKRTGARVLEGYGLTETSPTVSVMRIEEPSRPGSCGKPVPNV
                     EVAILDDDDRPVPAGETGEVCVKGPNVMKGYYKLPDATAEVMRGGWFHTGDMGHLDED
                     GYLYITERKKDLIIRGGFNIYPRDIEEIIYGNPKVQECAVVGVPDPSMGEEVVAYVVL
                     RPGESLTEDEVMSLCRENLAKYKTPKYVRFIDSLPKNPVGKIMKKDLRDRAKGEIPG"
     gene            complement(1581..2603)
                     /locus_tag="VM841_03310"
     CDS             complement(1581..2603)
                     /locus_tag="VM841_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89245.1"
                     /translation="MRRKATRIEEIIAAAARSDGRVYVRVGNGTPVAELWLHGPNLIA
                     ASAEGGERRLGARLLSTGHLASAELERSRTAAAKCGASLAVALAERVRRDVLTALAGD
                     EARYALAAALAAPQEEVSSEEDRSPEGLFPLLMDAADLLNRTHEFLAAVREAQAQIPP
                     ASIPVATEAPSPDMALGPEEWALLSRADGTVTVAMLADECGFPQPEASLIVTRLASMN
                     LLRVEEGFPDSSELLAELEAAEDLAAISAAFEHVAAFPEPDPEFEAEAPEPGFPEASS
                     PEPEAVVVQMPAPRGSGNGVAATLPGDPPASPHGLPAGNGDRTVDTQALLRELASIAR
                     TPEEKR"
     gene            2719..3246
                     /locus_tag="VM841_03315"
     CDS             2719..3246
                     /locus_tag="VM841_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HVL89246.1"
                     /translation="MTGKRRIDRILSETYLEGLADRPMPEVRSMRNDCREEESVLSFE
                     RRMLHGRMDLLRAELARRADGKEGSLIDELAKILAGDERPEDRGGFPGPDPRPGVDHP
                     KRRIEKLISDDTLANVGSMSPDEIEETLGLLSDAEREASQSRRQVQRVLDAINAEIAR
                     RYKSGEADPADALSS"
     gene            3322..3711
                     /locus_tag="VM841_03320"
     CDS             3322..3711
                     /locus_tag="VM841_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL89247.1"
                     /translation="MRAADPARVLVVDDDPVIREVVSAVLSDATCELLVARSGTEALD
                     AAQLHRPHVVVLDVMMPGMNGFEVCRRIRALPELQGTVVVMLTALDTQTSRDEGMDSG
                     ADLYLTKPFSALDLLGAVHEAVARVVR"
     gene            3698..4429
                     /locus_tag="VM841_03325"
     CDS             3698..4429
                     /locus_tag="VM841_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVL89248.1"
                     /translation="MSFASAQRADEMSEAPEKEWFLREQMLSLAHDLSTLYSRERDKT
                     ERLEATLEELEESYGATVRMLAFVIEAKDPHVHSHLERSFKYAKALARRIDPALAGDP
                     AVQHGFLLHDIGKVGIPERILGKPGPLTGEEFEVMKTHPMIGSRIVEPVRALRAAADV
                     IEGHHERWDGGGYPRGRKGEEIPLAARIFSIADSFDAMTSDRPYRKALTLEHALDEVR
                     AGAGTQFDPDCVRAFLDMDWAAASG"
     gene            4442..5392
                     /locus_tag="VM841_03330"
     CDS             4442..5392
                     /locus_tag="VM841_03330"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HVL89249.1"
                     /translation="MGGSTDPILAGVVRDDVVESVHILDAVVVDAHERVVFAAGEPEH
                     TLYWRSALKPIQASVCIAQGWRPDGDEQIAIACASHNAEEAHLAAVRSIFDSAGLSQD
                     ALRCPPALPLSPLVAAHAGAPARILRDCSGKHAAFLATCVARDWPLDTYLDPAHPLQR
                     AIVARASELTGEAASTGVDGCGAPTPAGRLRAIAAAFGRGIAGDPHVHRSMRAHPFLV
                     AGTGRVDTAVMQATGGDLVLKGGAEGLMCFASPAAGLAGAVKARDGSARAVAPATVAV
                     LVRTGLLGEPLPAALAEYAAPPVLGGGEARGTVQIQRFSG"
     gene            5461..5967
                     /locus_tag="VM841_03335"
     CDS             5461..5967
                     /locus_tag="VM841_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017242413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL89250.1"
                     /translation="MKVQQLGKFIRDQRRTASLSLRKLSSLAGVSNPYLSQIERGLRR
                     PSADILQAIAKALSISAETLYIKAGILDERPEDADVVGMIRRDSTVSEKQKQILIELY
                     QSFKAPAPPTPRTRDASQPKARKSATATKSRPAGKARATAKKAAPAKKKPAAKKPAAK
                     KTTRRKTT"
     gene            6065..6634
                     /locus_tag="VM841_03340"
     CDS             6065..6634
                     /locus_tag="VM841_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89251.1"
                     /translation="MSIARKTVYAAAGAAGEALDRAKKLPKRAAEVSGKLTESTKDLT
                     KKTSSLPSTAASLPGKAAGLTKKATKRAAELPGVAAGIVKTAGSLPKQAGQLPKVAAD
                     VAGKATKTVRGFATETAKGATKRFDEFATRGEKLLARNGKPAAKKKATTAKKRTSKPK
                     SSANGSSKTTTVAAAETTNGVAQTAEQLV"
     gene            6749..>7104
                     /locus_tag="VM841_03345"
     CDS             6749..>7104
                     /locus_tag="VM841_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89252.1"
                     /translation="MPVTVHLELGEIDSLGPSWHELLAADPSASIFQTPEYAHVWWEE
                     FGALRALALLEIRDGDRLAGIAPLSMEPDGSVHFVGDPSITDYLAPISRPQDRDAVAA
                     VLVESAVALEGFTRLSL"
BASE COUNT         1175 a         2468 c         2418 g         1043 t
ORIGIN      
        1 gaaccgaacc gaaccgaacc gaaccgaacc gaaccgaacc gggatcgagg gagcctgcga
       61 atgaacgtcg ccagccttgc cgagcgcaac gtcgagaagt tcgggaccta cacgcaactg
      121 tggttcgaag gccgcgagtt caccaacgtc gagatgctgg agaaagccgc gcgcttcgcg
      181 accgcgctca aggatgcggg ggtcaaggaa ggcgaccggg tcggtgtgat gctgccgaac
      241 ctgcccgagg tcggcatcgc ctacggcgcg ctgcttcgca tgggcgcgat cgtcatgccc
      301 atggtcttct tgctcgcggt gcccgagatc cgtcacatca tcgaagacag cgaggcgacg
      361 atcatcatca ccagccccga gttccggccg aacgtggcgg ccgcgtgcga gggtattgcg
      421 aacccacccc gcacgatcgt ggtcggtgac accacgccgg agggctgcct gtcgtacgcg
      481 cagatggtgg agagcccggc cgagccgcat ccggttgtcg agcgcggcgc tgacgacctc
      541 gccgtgatca tgtacacctc cggcacgacc ggccatccga agggcgtgat gctcacgcac
      601 ggcaacctgc tgttcaacgc cgagaactcg gccaaggccg tggatctgcg cgacggagac
      661 gtgtcggtcc agggcttgcc gcaggcgcac ctgttcgggc tcgcgtcttc gatcaccggc
      721 agcttgttca aggtcaaagg catcctgctg cgatggttca ccgccgaagc gttcttcgag
      781 gcggtcaaca cgcatcgcgc gaactccagc ggtgtcgtcc cgacgatgct cacctacatg
      841 ctgtcgcacc cgcagttcga cgacgtggac tggtcgagca tgcgctgggt cgtgtgcggc
      901 gccgcgccgc ttccggtcga ggtcgcggcc gagttcgaaa agcgcaccgg cgcgcgggta
      961 ctcgaaggct atggcctgac cgagaccagc ccgaccgtga gcgtcatgcg catcgaagag
     1021 ccgtcccggc cgggatcgtg cggcaagccc gttcccaacg tcgaggtcgc gatcctcgac
     1081 gacgacgacc ggcccgtccc ggccggtgag accggcgagg tgtgcgtcaa gggaccgaac
     1141 gtgatgaagg gctactacaa gcttcccgac gcgacggccg aggtgatgcg cggcggctgg
     1201 ttccacaccg gcgacatggg gcatctcgac gaggacggct acctctacat caccgagcgc
     1261 aagaaggacc tgatcatccg cggcggcttc aacatctacc cgcgcgacat cgaggagatc
     1321 atctacggaa accccaaggt gcaggagtgc gcggtggtcg gcgtcccgga cccgtcgatg
     1381 ggtgaggagg tcgtggccta cgtcgtgctt cggccgggcg agtcgttgac cgaggacgag
     1441 gtcatgagcc tgtgccgcga gaacctcgcc aagtacaaga cccccaagta cgtgcgcttc
     1501 atcgactcgt tgccgaagaa cccggtcggc aagatcatga agaaggacct gcgggaccgc
     1561 gccaagggcg agatccccgg ctagcgcttc tcctcgggcg tgcgcgcgat cgacgcgagc
     1621 tcccggagca gggcctgggt gtccacggtc cggtcgccgt tccccgccgg caggccgtgc
     1681 ggtgaggccg gcgggtcccc cggcagcgtg gccgctacgc cgttgccgct cccgcgcggg
     1741 gccggcatct gaaccaccac ggcttccggc tccggtgacg atgcctcggg gaagccgggc
     1801 tcgggcgcct cggcttcgaa ctcgggatcg ggctcgggaa acgcggcgac atgctcgaat
     1861 gcggcgctga tcgcggcaag atcctcggcg gcttccagct cggcgagaag ctccgaagag
     1921 tcggggaatc cctcttcgac ccggagaagg ttcatcgagg cgagccgcgt cacgatcagc
     1981 gatgcctccg gctgcgggaa gccgcactcg tcggccagca tcgccacggt gacggttccg
     2041 tcggcgcgcg acagcagcgc ccattcctct ggcccgagcg ccatatccgg cgacggagcc
     2101 tcggtcgcga ccgggatcga ggccggtggg atctgcgcct gtgcctcccg gaccgcggca
     2161 agaaactcgt gtgttcggtt gagcagatcg gcggcgtcca tcaaaagcgg gaagagccct
     2221 tcgggggagc ggtcctcctc ggaggagacc tcctcttggg gcgcggcgag cgcggcggcg
     2281 agcgcgtagc gcgcctcgtc gccggcgagg gcggtcagca cgtcgcgccg gacccgctcg
     2341 gccagcgcca cggccagcga cgctccacac ttggcggccg cggtgcgcga tcgctcgagc
     2401 tcggccgacg cgagatggcc ggtgctgagc aggcgggctc cgaggcgccg ctcgccgcct
     2461 tcggcggagg ccgcgatcag gttcggcccg tgcagccaca gctcggcgac cggagtgccg
     2521 ttgcccaccc ggacgtagac gcggccatcc gagcgcgcgg ctgcggcgat gatctcttcg
     2581 atgcgcgtcg ctttccgtct catggatccc cgtttgtttg tcggcacccg cacgcccccg
     2641 gtgaaggccg cgagcaagcc cgaagccagc gagcggctgc tacagtggcg gccgggaaag
     2701 gccgaggaag acagggatat gaccgggaag cgccgaatcg accggattct ttcggagacc
     2761 tacctcgagg ggctcgccga ccgaccgatg cccgaggtca ggtcgatgcg caacgactgc
     2821 cgggaggaag agtcggttct gtcgttcgag cgccgcatgc tccacggccg catggacctg
     2881 ctccgggccg agctcgcgcg ccgcgccgac ggcaaggaag gctcactgat cgacgagctg
     2941 gccaagatcc tcgccggcga cgagcgtccc gaagaccgtg gcgggttccc cggtcccgat
     3001 ccgcgaccgg gggtcgatca tcccaagcgg cggatcgaga agctcatctc cgacgacacg
     3061 ctcgccaacg tcgggtcgat gtccccggat gagatcgagg agacgctcgg cttgctctcc
     3121 gacgccgagc gcgaggcgtc ccagtctcgg cggcaggtcc agcgggtcct ggatgcgatc
     3181 aatgcggaga tcgctcgccg gtacaagtcc ggcgaagccg acccggcgga cgctctgtcg
     3241 tcctaatcga cgcaacgggc catcgcatct gactcgttgt gcctatagac gcggccaccc
     3301 aatccccagg ataaagggtt catgcgcgcg gccgatcccg cccgagtcct cgtagtcgat
     3361 gacgatcccg tgattcggga ggtcgtgtcg gccgtcttgt ccgacgccac gtgcgagctg
     3421 ctcgtagctc gaagcggtac ggaagcgctc gacgcggcgc agctgcatcg tccccacgtc
     3481 gtcgtgctcg acgtgatgat gcccggcatg aacggcttcg aggtctgccg gcgcatccgc
     3541 gctctgcccg agctgcaggg cacggttgtg gtgatgctca ccgctctcga cactcaaaca
     3601 tctcgcgacg agggtatgga cagcggagcc gacctgtatc tcaccaagcc gttctccgcg
     3661 ctggacctgc tcggagccgt gcacgaggcg gtggcgcgtg tcgttcgcta gcgcgcagcg
     3721 ggccgacgag atgtccgaag ccccggagaa ggagtggttc ctgcgggagc agatgctctc
     3781 gctcgcgcac gacctgagca ccctgtacag ccgtgagcgc gacaagaccg aacggctcga
     3841 ggcgacactg gaagagctcg aggagagcta cggcgccacc gttcgcatgc tcgcgttcgt
     3901 gatcgaggcg aaggatccgc acgtccactc gcacctcgag cggtcgttca agtacgcgaa
     3961 ggcgctcgcc cggcggatcg accctgcgct cgcgggcgac ccggccgtgc agcacggctt
     4021 cctgcttcac gacatcggca aggtcggcat tcccgagcgc atcctcggaa agccggggcc
     4081 gctgaccggc gaagagttcg aggtgatgaa gacgcacccg atgatcggct ctcgcatcgt
     4141 cgagcccgtg cgcgcgcttc gggccgctgc cgacgtcatc gaaggtcatc acgagcggtg
     4201 ggacggcggc ggctacccgc gaggacgaaa aggtgaggag atcccgctcg ccgcgcgcat
     4261 cttctccatc gccgactcct tcgacgccat gacctccgac cgcccgtacc ggaaggcgct
     4321 cacccttgag cacgcgctcg acgaggtgcg tgccggcgct ggaacgcagt tcgatcccga
     4381 ttgcgtgcgc gccttcctcg acatggattg ggccgcggcc tccggctagc atgacgccgc
     4441 cgtgggcggc tcgaccgatc cgatcctcgc cggcgtcgtc cgggacgacg tcgtggaaag
     4501 cgttcatatc ctcgacgcgg tcgtcgtcga cgcccacgag cgtgtcgtct tcgcggccgg
     4561 cgagcccgag cacaccttgt actggcgatc cgcgctcaag cccattcaag cgagcgtgtg
     4621 catcgcgcaa ggatggcggc cggacggcga tgagcagatc gcgatcgcgt gcgcctcgca
     4681 taacgccgag gaggcgcatc tggcggccgt gcgctcgatc ttcgattccg ccggcctgag
     4741 ccaggacgcc cttcgctgtc cgcccgcgct gccgctgtcg cccttggtcg ccgcgcatgc
     4801 cggcgcgccc gcgcggatcc tgcgcgattg cagcggcaag cacgcagcgt tcctcgcgac
     4861 gtgcgtggcg cgcgactggc cgctcgacac ctatctcgat cccgcgcatc cgctgcaacg
     4921 cgcgatcgtg gcgcgcgcga gcgagctgac cggcgaagcg gcctcgacgg gcgtggacgg
     4981 atgcggagcc ccgacgcccg ccggccgcct ccgtgcgatc gcggcggcct tcggtcgcgg
     5041 aatcgcgggc gacccccacg tgcatcgttc gatgcgcgcg catccgttcc tcgtcgcggg
     5101 gacgggccgc gtcgacaccg ccgtcatgca ggccaccggc ggcgatctcg tgctcaaggg
     5161 gggcgccgag gggctgatgt gcttcgcctc gcccgccgcg ggcttggccg gagccgtcaa
     5221 ggcccgcgac ggctccgcgc gggccgtcgc ccccgccacg gtcgccgtcc tggtgcgcac
     5281 cgggcttctc ggcgagccgc tcccggccgc cctggccgag tacgcagcac cccccgtcct
     5341 ggggggcggc gaagctcgcg ggaccgtgca gatccagcgg ttttccggct gatctcatcc
     5401 gcttgcagcc gctatgcagg ccgcttgcaa acgttataca cctgcgatag cgtggcatgg
     5461 atgaaggttc agcagctcgg caagttcatc cgcgaccagc ggcgcacggc gtcgctgtcg
     5521 ctccgcaagc tttcttcgct cgccggcgtc tcgaacccgt acctttcgca gatcgagcgc
     5581 ggccttcgcc ggccgagcgc cgacatcctg caagcaatcg cgaaggcgct gtcgatctcg
     5641 gcggagacgc tctacatcaa agccggcatc ctcgacgagc gtcctgagga cgccgacgtc
     5701 gtgggcatga tccggcgcga ctcgaccgtg tccgagaagc agaagcagat cttgatcgag
     5761 ctctaccagt cgttcaaagc gccggcgccg cccacgccgc ggacccgcga cgcctcgcag
     5821 cccaaggcgc ggaagagcgc gacggccacg aagtctcgcc ctgccggcaa ggcccgcgcg
     5881 acggcgaaga aggccgcgcc ggccaagaag aagcccgcgg cgaagaagcc cgcagcgaag
     5941 aagaccacgc ggagaaagac cacatagcca cggcgggggg gcggagattt ggacgtgctc
     6001 caaagccctc acccggctcc ccttcgttcc cccgccgcaa aaaccgcctc cgaaggaggg
     6061 acgcatgagc atcgcccgca aaaccgtgta tgccgccgcc ggcgccgccg gtgaagcgct
     6121 cgaccgcgcg aagaaactcc ccaagcgcgc ggccgaggtg tccggcaagc tgaccgaaag
     6181 caccaaggac ctcaccaaga agacgtcctc gcttccgtcg accgccgcgt ccctgcccgg
     6241 caaggctgcc gggctgacga agaaggcgac gaagcgtgcc gccgagctgc ccggcgtcgc
     6301 cgccgggatc gtcaaaactg ccggctcgct ccccaagcag gccggccagc tcccgaaggt
     6361 ggccgccgac gtcgccggca aggcgacgaa gacggttcgt ggcttcgcca ccgagacggc
     6421 caagggcgcg acgaagcgtt tcgacgagtt cgccacgcgc ggtgagaagc ttctcgcgcg
     6481 caacggcaag ccggccgcca agaagaaggc gacgacggcc aagaagcgca cgtcgaagcc
     6541 gaagtcctcg gccaacggct cgtcgaagac gaccaccgtc gccgcggccg agacgaccaa
     6601 cggcgtcgcg cagaccgccg agcagctggt ctagccctcg atcaattcgc aagagcccgg
     6661 cataatcgcc gggctcttcg cgttgacgaa ggacatgact cgcagccggg ggacgcttgc
     6721 tcgcgggctg cgatacgctg cggcctccat gccggtgacc gttcacctcg agctcggcga
     6781 gatcgattcg ctcggccctt cgtggcacga gcttctggcc gccgacccct ccgcgagcat
     6841 cttccagacg cccgagtacg cgcatgtgtg gtgggaggag ttcggcgcgc tgcgcgcgct
     6901 ggcgctgctc gagattcggg acggcgaccg gctggccggc atcgccccgc tgtcgatgga
     6961 gcccgacggg tccgtgcact tcgtcggcga tccctcgatc accgattatc tcgcgccgat
     7021 ctcccgtccg caggatcgcg acgcggttgc cgcggttctc gtcgagagcg ccgtcgcgct
     7081 cgagggcttc acgcggctgt cgct
//
LOCUS       DATLFO010000098        11049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_92327, whole genome shotgun sequence.
ACCESSION   DATLFO010000098 DATLFO010000000
VERSION     DATLFO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92327"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..842
                     /locus_tag="VM841_03350"
     CDS             435..842
                     /locus_tag="VM841_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89253.1"
                     /translation="MLGERIGDTIGEVTGLRVLEPVEGQPRIEASYRANGTILGVHVT
                     DMGTFESVARPDGTLFGRGHGVMMTEEGELLTWSGDGVGTPKGGPGAVAWRGAIYVKT
                     TSDKLARLNGVAVLFEFDTDESNKVESTLHEWK"
     gene            complement(847..3330)
                     /locus_tag="VM841_03355"
     CDS             complement(847..3330)
                     /locus_tag="VM841_03355"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HVL89254.1"
                     /translation="MTDDIAVRRALRRAEQARTLSPDESAALLTARGDELERLLAVAR
                     RVRHAGRGPVVSFSRKVFIPLTRLCRDRCGYCTFATTPDRTPAFLTPDEAVAVAKAGA
                     RAGCKEALFTLGDRPESRWPEARAWLAERGYASTLDYVRAVTIRVIEETGLLPHLNPG
                     VMSYEEMARLKPVSASMGLMLETASERLFNTPGQAHFGSPDKKPSVRLRCIEDAGRLA
                     IPFTTGILIGIGETLAERVEALLKIRALHARYGHVQEVIVQNFRAKPETAMEGAPEPG
                     EDDYLTTIAVARLLLGPRTTVQAPPNLSDERFPRLLDAGIDDWGGVSPVTPDHVNPEK
                     PWPHLDVLQRETMRRGLTLRERLTIYPGFALKPDPWLSGRMQAPVAALRAPDGYALPR
                     SPAPHAWQDPDAPDATPMSAGIAAIVAGDDATALLHRDLRADTDEVHGSWELLEVDLS
                     RAWGSRGATPRSEVRAALARAESGAEPTDAEAVALFTATGEDLEALCGLADAMRADAV
                     GDEITYVRNRNINFTNICYVGCRFCAFAQRRNDPDAYTISLEEVADRAQEAWDQGASE
                     VCIQGGIHPDLPGDHYFRIVRAIKARVPGIHVHAFSPMEVLNGASRLGISFREWLQEA
                     KAAGLGTIPGTAAEILDDEVRWALTKGKLPADTWETIVRTAHGLGIRSSSTIMYGHVD
                     APPHWVGHIRRLRGIQRDTGGFTEFVPLPFVHQNAPIYLAGKARPGPTHDDNRRMHAV
                     ARIMLRGAIDNVQVSWVKLGLDGSRRMLQGGANDFGGTLMEETISRMAGASHGVRMEV
                     EQFHEAIRSIGRVPAERTTTYERVATRAG"
     gene            3439..4377
                     /gene="cofD"
                     /locus_tag="VM841_03360"
     CDS             3439..4377
                     /gene="cofD"
                     /locus_tag="VM841_03360"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HVL89255.1"
                     /translation="MITVLAGGAGAARFLRGLARVVDEAAVTVVSNTGDDIHVYGAHV
                     SPDLDIVTYTLAGIVDGERGWGIDGDTFRVLDGLRALGVDTWFTLGDADFATCLARAA
                     HLREGGTASAFAARLAGTRGVRARLLPMTDDPVATIVTAKSGEAMHFQEYWVRRRAAD
                     PVAAVALSGAADARPAPGVLEAIEAADAVLIAPSNPIVSIGTILAVPGIREALRSTTA
                     PVVGVSPIVGGAVVRGMADKLMSGLGHEVSCTAVAALYEDLIDGFVIDLVDGAAAPRV
                     AEAGGCVAEVAQTMMRTVEDAAALAKTTLALAERIR"
     gene            4374..5096
                     /locus_tag="VM841_03365"
     CDS             4374..5096
                     /locus_tag="VM841_03365"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF009810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HVL89256.1"
                     /translation="MSVEILAVDGMPEIAEGDDIAAMIASRATLRDGDVVVVTQKVVS
                     KAEGRLVAIDPSDPEGERARWAEREAVRIVARRGPVLITETAHGFVCANSGVDASNVE
                     PGFLALLPPDPDASAARIRAGLREATGADVAVIVSDTFGRAWRHGQIDVAIGAAGIEP
                     IRDYVGAKDSFGVEMHSTQIAVADEIAAAAELVMGKTAGVPVAIVRGLDVRGDGSARS
                     LVRRADEDLFRSGEGGEPDGDR"
     gene            complement(5093..5812)
                     /locus_tag="VM841_03370"
     CDS             complement(5093..5812)
                     /locus_tag="VM841_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89257.1"
                     /translation="MPKRFSQIDPLEIERAARRVPGVAFAEVGDAADGRRGLSIVLYQ
                     DAPGEETAGLVEVALREIGVEVQAGDVRIAGYAGQMPGAASGVKVIDLGKGTWATESE
                     SDAVTPVSLGSPGTGSRTIGLVSVTVSRSRDRCRARVEVDAGDGVHVGDASGPATDMR
                     MRRSVSEAAAKAAATALGLGAPDVEHVALITLGDVACAIVVIGAPGESPERRVGSSLV
                     TGDAWHSFAMAALQAAAALAD"
     gene            6036..6821
                     /locus_tag="VM841_03375"
     CDS             6036..6821
                     /locus_tag="VM841_03375"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HVL89258.1"
                     /translation="MAEKPTKRERKEEAKRRRLEEMKRRQRRARMRKLYTLGIVGAII
                     TAIVAGVLISRASNENARKRAEKLAAEAGCEPPAQYPSEGSDHITPPSTVSYKTDPPT
                     SGAHYAQAASPPAPGPTGVLASALPNEAQVHNLEHGHILIQYQQDALTESQIKSLTAL
                     VESDPGWIQLAPRENRDAKVAFTAWQVLQKCNEPNDKIAEAAAEFVKRFKNQAPESVA
                     ANPVFQSLPPAPQPDGEPDGEQTGSPTGSPAGTASPTASPTAS"
     gene            complement(6871..8814)
                     /locus_tag="VM841_03380"
     CDS             complement(6871..8814)
                     /locus_tag="VM841_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89259.1"
                     /translation="MKTRGPSAGRAIVATLMVACLTAAAAVAATPAVPRPALGEASDV
                     VPGQAVVRFAATASAAARAAALGRAGARPVRALLVRDHALVAIAGPLPQALRRLDAQR
                     AIVRAEPNRLARIQLGSPNDPCTSTFASPPCPSVWHLSAIAAQPSWARYPGLYYPPAS
                     KLAIPAVHRVTVAVPDTAVQPSNVDFRNGGSSEDAALGGQLDIASMNGFAGLPPTSGP
                     AAYHGTFVAGLVAASSNNSFASVGVGYHADLLPLAVVNSDTGGAETAKLADAIVYAHQ
                     RGARVINLSLGLLSPDGTVESAIRQVTTGPSPALVVAAAGNFGNNQPFYPAWFDNVLA
                     VGGTDQSDRKASCSNWGPKISVVAPAKSVASITSDGFMVAPDCGTSAATPQVAGLAAA
                     LFAQVPSRTPAQVRAIIEQTADDLGEPGRDDVFGHGRINADRALGLGSGPQTSRLIAT
                     PVRANGGTSTVTAVATAATPVAAAEMFLNRLPADAADRGFPVGAADGVFDSGFETLQA
                     NFTAGSLAAGPHRVYVRARDASGTWGPLASAVMYADGVAPTIAQLQATNVVRPLDGAS
                     TITFYAADDYSPAINYDVEVTLAGQPGLRVWRAERIAGVAGLQRVTWTPRTIEAPGSY
                     TVRVMLRDQVGNTVSAATTFLMV"
     gene            complement(9024..10571)
                     /locus_tag="VM841_03385"
     CDS             complement(9024..10571)
                     /locus_tag="VM841_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89260.1"
                     /translation="MTFGSWTRWRVALAMLCVAVAIAPLNPGIADIRGGLVNGYSDHF
                     DYAGVTPTNDVPIDQLQWQITPTASNGTPIPNVWMVGSVQTSTIGGPGSEIKALKQRT
                     TGAAASEPVAFVRNGNWQNLVVQVRAAYDGNSQNSGVGIVFRAPVDPVTGTADKNNFY
                     LFTTSSQVPHPELCPTGRCVALFKRIGGAYFLATMKPTFLNFVNANGPAESHVYKVVM
                     SGNRIQAYVDGRLLIDVTDTPGDDISGTRFSMPGPMFANGTVGLRTSNTRAWFDDFVV
                     MGDSERAYEGRAAAMTTFGQVGAGSTGAVLQATAADTNFQYHDHDFPTGTGTEGTFIG
                     PTSPGNGFTGGALVQTRGQNGTTSSSAQLFGFSGTISQSMPDGSIVDVTLISDTISAR
                     ASTNCDDPPSTRVELLNFSYLVVVKNAQGLPILNEGKVSQVVPPPNTVISDPQSNGGY
                     VRIVLNSQQTTTDPKRADVSGIQIDFLQRPLTVSSGQSTIADTGITFASVQLANVVAG
                     RLCMPFI"
     gene            complement(10832..>11049)
                     /locus_tag="VM841_03390"
     CDS             complement(10832..>11049)
                     /locus_tag="VM841_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89261.1"
                     /translation="PLQRYFFVRNPYLLLRTHLAPPARWLAEARATRRYADWARKSPP
                     PLRRAIMLGMRHGLLGRFGPPPPELMP"
BASE COUNT         1644 a         3778 c         4033 g         1594 t
ORIGIN      
        1 ggggggcagg cgccgccgca tcgcgccgcg tcctgcccac ggatcgctcg tggcccggaa
       61 aaatcgcccg gagcctgggg ggatccggcc gcgcgcgagg taggatttca tcatcgaggg
      121 catcccccct cgagcgcatc ccccgcaggg aggggcccca cccctccctg tttctttttg
      181 gctcgccttc tcggcccctt tcgtctccgg tccgttcttc gggccagcac cttttcggcg
      241 agccgccgat tccgtccggg cccccgtcac cgccccgatg tggcccgctc cgggacgtca
      301 tgcgccgggg cggaaatgcc tccagctacc tattccggcc cggggcaggc ggtctctagc
      361 atcactccgg gggcggccct gccccggtcc ggacttcgcc ggatccggcc ggccgcgccg
      421 aaagggtgaa ggcaatgctc ggagagcgca tcggagacac gatcggtgag gtaaccggcc
      481 tgcgggtgct ggagccggtg gagggacagc ctcggatcga ggcgtcgtac cgcgcgaacg
      541 gaaccatcct gggcgtgcat gtgaccgaca tgggaacctt cgagtcggtc gcccgcccgg
      601 acggcacgct cttcggacgg ggccatggcg tgatgatgac cgaggagggc gagctcttga
      661 cctggtccgg cgacggagtc ggaacgccga aaggcggacc gggcgcggtc gcatggcgcg
      721 gtgccatcta cgtcaagacc acgagcgaca agctcgcgcg cttgaacggc gtcgcggtcc
      781 tgttcgagtt cgacaccgac gaatcgaaca aggtcgagtc gaccctgcac gagtggaagt
      841 agcggctcag ccggcgcgag tcgcgacgcg ttcgtaggtg gtggtgcgct cggccggcac
      901 gcgcccgatc gatcggatcg cttcgtggaa ctgctcgacc tccatgcgaa cgccgtggct
      961 cgcgccggcc atccgcgaga tcgtctcttc catcagcgtg cccccgaagt cgttggcgcc
     1021 gccctgcagc atccggcggg agccgtcgag cccgagcttg acccacgaaa cctgaacgtt
     1081 gtcgatcgcg ccgcgaagca tgatgcgcgc gaccgcgtgc atacggcggt tgtcgtcgtg
     1141 cgtgggcccc gggcgggctt tgccggcgag atagatcggc gcgttctgat gcacgaaggg
     1201 cagcgggacg aactcggtga acccgccggt gtcgcgctgg atgccgcgca ggcggcggat
     1261 atggccgacc cagtgcggcg gcgcgtccac gtgcccgtac atgatcgtgc tcgacgagcg
     1321 gatcccgagg ccgtgcgcgg tgcgcacgat ggtttcccat gtatcggccg gaagcttgcc
     1381 tttcgtcagc gcccagcgca cctcgtcgtc gaggatctcg gcggccgttc cggggatcgt
     1441 tccgagcccg gccgccttcg cctcctggag ccattcacga aacgagatgc ccagccgcga
     1501 tgcgccgttg aggacctcca tcgggctgaa cgcgtgcacg tggatcccgg gaacgcgcgc
     1561 cttgatcgca cgcacgatcc ggaagtagtg gtcgccggga aggtccggat ggatgccgcc
     1621 ctggatgcac acctctgatg cgccctggtc ccacgcttct tgcgcgcgat ccgcgacctc
     1681 ctcgagcgag atggtgtagg cgtccgggtc gttgcgccgc tgcgcgaagg cgcagaagcg
     1741 gcagccgacg tagcagatgt tggtgaagtt gatgttgcgg ttgcgcacgt aggtgatctc
     1801 gtcgccgacg gcgtcggcgc gcatcgcgtc ggccaggccg cacaacgcct ccaggtcctc
     1861 accggtcgca gtgaacagcg cgaccgcttc cgcgtcggtc ggctcggcgc cggattcggc
     1921 gcgcgcgagc gcggcgcgaa cctcgctgcg gggcgtcgcg ccgcgcgatc cccaggcgcg
     1981 cgacaggtcc acctcgagaa gctcccagct gccgtgcacc tcgtcggtgt cggcgcgaag
     2041 gtcgcggtgc agcaacgcgg tcgcgtcgtc gccggccacg atcgctgcga tcccggcact
     2101 catcggcgtg gcgtcgggtg cgtcgggatc ctgccaggca tgcggtgcgg gtgagcgcgg
     2161 cagcgcgtag ccgtcgggcg cgcgcagcgc cgcgaccggc gcctgcatcc gaccggaaag
     2221 ccacgggtcg ggcttgagcg cgaaaccggg atagatcgtg agccgctcgc gcagcgtcag
     2281 gccgcgccgc atcgtctcgc gctgcagcac gtccaggtgc ggccacggct tctcgggatt
     2341 gacgtggtcg ggcgtcaccg gcgacacgcc gccccaatcg tcgatgccgg cgtccagcag
     2401 tcgcgggaag cgctcgtcgg acaggttcgg cggggcctgg acggtggtgc gcggaccgag
     2461 cagcagccgc gcaacggcga tcgtggtcag atagtcgtcc tcacccggct cgggagcgcc
     2521 ctccatcgcg gtctcgggct tcgcgcggaa gttctgcacg atcacttcct gcacgtggcc
     2581 gtaccgcgcg tgcagcgcgc ggatcttgag cagcgcctcc acgcgctcgg cgagcgtctc
     2641 gccgatgccg atcaaaatgc cggtggtgaa cgggatcgcc agacgaccgg cgtcctcgat
     2701 gcaacgcagg cgtaccgacg gcttcttgtc gggcgatccg aagtgggcct gacccggcgt
     2761 gttgaagagg cgctcgctgg cggtctcgag catcaacccc atgctggcgc tcaccggctt
     2821 gaggcgggcc atctcctcgt agctcattac gcccgggttc agatgcggca gcaggccggt
     2881 ctcttcgatg acgcggatcg tgaccgcgcg cacgtagtcg agcgttgagg cgtagccgcg
     2941 ctcggcgagc caggcgcgcg cctcgggcca gcgggactcg ggacggtccc ccagggtgaa
     3001 cagcgcctcc ttgcaccccg cccgtgcgcc ggccttcgcg acggcgaccg cctcgtcggg
     3061 cgtcaggaac gcgggcgtgc ggtccggcgt cgtggcgaat gtgcagtagc cgcaccggtc
     3121 gcggcacaga cgcgtcagcg gaatgaagac cttgcgggag aacgacacga ccgggccgcg
     3181 gccggcgtgc cggacgcggc gcgcgacggc cagcagccgc tcgagctcgt cgccgcgggc
     3241 ggtcaggagc gcggcggatt catcgggcga gagcgtgcgt gcctgctcgg cccggcgcaa
     3301 tgcacggcga accgcgatgt cgtcggtcac gtcgcgcgga gcctaccatc ggcccgggcc
     3361 gtccggtccg gcattgtcgg gcgacgatgc gttcggcagg cgagtccgaa gccgcggatg
     3421 ctcggctagg ctggcgagat gatcacggtc cttgccggcg gtgccggcgc ggcgcgcttc
     3481 ctgcgcgggc tggcccgcgt cgtggacgaa gcggccgtca cggtcgtttc gaacaccggt
     3541 gacgacatcc acgtctacgg agcccacgtg agcccggatc tggacatcgt gacctacacg
     3601 ctcgcgggga tcgtggacgg cgagcgcggc tggggcatcg atggggacac gttccgggtc
     3661 ctcgacggcc tgcgcgcgct tggcgtggac acctggttca cgctcggcga cgccgacttc
     3721 gccacctgcc ttgcccgcgc cgcgcacctg cgcgagggcg gcaccgcgtc ggcgttcgcg
     3781 gcccgcctgg ccgggacccg cggcgtgcgc gcccggctgc tgcccatgac cgacgacccg
     3841 gtcgccacga tcgtgacggc aaagagcggc gaagcgatgc attttcagga gtactgggtc
     3901 cgccgccgcg ccgccgatcc ggtcgccgcg gtcgcgttga gcggcgcggc ggatgcgcgc
     3961 ccggcgccgg gcgtgctcga ggcgatcgag gccgccgacg cggtgctgat cgcgccgtcg
     4021 aacccgatcg tttcgatcgg caccatcctc gcggtgcccg gcatccgcga ggcgctccgc
     4081 tcgaccacgg caccggtcgt gggggtctcc cccatcgtcg gcggcgcggt cgttcgcggg
     4141 atggcggaca agctgatgag cggcctcggc cacgaggttt cctgcacggc cgtggcggcg
     4201 ctctacgagg atctgatcga cgggttcgtg atcgatctgg tggacggcgc tgcggcgccg
     4261 cgggtcgcgg aggccggggg ctgcgtggcc gaggtggcgc agacgatgat gcgcaccgtc
     4321 gaggacgccg cggcgttggc caagaccacg ctcgcgctcg cggagaggat tcggtgagcg
     4381 tcgagatcct cgccgtcgac ggcatgcccg agatcgccga gggcgacgac atcgcggcga
     4441 tgatcgcgtc gcgcgcgacg ctgcgcgacg gcgacgtggt ggtcgtgacg cagaaggtcg
     4501 tgtcgaaggc cgaaggccgg ctcgtggcga tcgatccgtc ggatcccgaa ggcgaacggg
     4561 cccgatgggc cgagcgcgag gcggtgcgaa tcgtggcgcg ccgaggcccg gtgctgatca
     4621 ccgagaccgc gcacgggttc gtgtgcgcca actccggcgt ggacgcgtcg aacgtggagc
     4681 cggggttcct ggcgctgctg ccgccggacc cggacgcgag cgccgcgcgc atccgcgcgg
     4741 ggctgcgcga ggcgaccggc gccgacgtcg cggtgatcgt gtcggatacc ttcggccgcg
     4801 catggcggca cggccagatc gacgtggcga tcggcgcggc ggggatcgag ccgatccgtg
     4861 actacgtcgg cgcgaaggat tcgttcggcg tcgagatgca ctcgacgcag attgcggtcg
     4921 ccgacgagat cgccgccgcg gccgagctcg tgatgggaaa gacggccggg gttcccgtcg
     4981 cgatcgtgcg cgggctcgac gtgcgcggtg acggctcggc gcgctcgctg gtccgccgtg
     5041 cggacgaaga tctgttccga agcggcgagg gcggcgagcc ggacggcgat cgctagtccg
     5101 cgagcgcggc ggccgcctgg agcgcggcca tcgcgaacga atgccacgcg tccccggtca
     5161 ccagcgacga tccgacccgg cgctcgggcg actcgcccgg cgcgccgatg accacgatcg
     5221 cgcatgcaac gtcgcccagc gtgatcagcg cgacgtgctc gacgtcgggt gcgcccagcc
     5281 ctaacgcggt cgcggccgcc ttcgccgccg cctcgctgac gctgcggcgc atgcgcatgt
     5341 cggtcgcggg accggacgcg tcgccgacgt gcacgccgtc gcctgcgtcg acctcgaccc
     5401 gcgcacggca tcggtcgcgt gagcgcgaga cggtgaccga gaccagcccg atggtgcggc
     5461 tgcccgtgcc cggcgacccg agcgacaccg gcgtgacggc atcggactcg ctctcggtcg
     5521 cccaggttcc cttgccgagg tcgatgacct tgaccccgga cgcggcgccc ggcatctgtc
     5581 cggcgtagcc ggcgatgcgc acatcgcctg cttgaacctc gacgccgatc tcgcgcaggg
     5641 caacctcgac cagaccggcg gtctcctcgc cgggcgcgtc ctgatacagg acgatgctca
     5701 gcccgcgacg gccgtcggcc gcgtcgccca cttcggcgaa cgcgaccccc ggcacgcgac
     5761 gggcggcgcg ctcgatctcg agcgggtcga tctggctaaa acgcttgggc acccacgcac
     5821 cccccgaatt gcaggcattc gacggacagg tggccgctcc ctgctcgccc gggcccccgc
     5881 aaactaccgg ttcgactcaa agttcccgaa cccgccgggt ttggcggccg gggccggggg
     5941 ccgtggcgtc ggagcggggg gtttgctata aagccccttt gtgcccccac cccaaattgt
     6001 gaacggggcc gacgactgcc gggagattcc acgacatggc cgagaaaccg acgaagcgcg
     6061 agcgcaaaga ggaagcgaag cgccgccgac ttgaagagat gaagcgccgt cagcggcggg
     6121 cgcgcatgcg caagctctac acgctgggca tcgtcggagc gatcatcacc gcgatcgtgg
     6181 ccggcgtgct gatctcgcgc gcgtcgaacg agaacgcccg caagcgcgcc gaaaagctcg
     6241 ccgccgaagc cggatgcgag ccgcccgcgc agtatccgag cgagggcagc gaccacatca
     6301 ccccgccgag caccgtgtcc tacaagaccg acccgccgac ctcgggcgcg cactacgccc
     6361 aggcggcgag cccgccggcg ccgggcccga cgggcgtgct ggcatcggcg ctgccgaacg
     6421 aggcgcaggt gcacaacctc gagcacgggc acatcctgat ccagtaccag caggacgcgc
     6481 tgacggagtc gcagatcaag tctctgaccg ctttggtcga gagcgacccg ggctggatcc
     6541 agctggcgcc gcgcgagaac cgcgacgcga aggtcgcgtt cacggcctgg caggtgctgc
     6601 agaagtgcaa cgagcccaac gacaagatcg cggaggcggc cgccgagttc gtcaagcggt
     6661 tcaagaacca ggcgcccgag tcggtcgcgg ccaacccagt gttccagtcg ctgccgccgg
     6721 ccccgcagcc ggacggggag ccggacgggg aacagaccgg ttcgcccacc ggttcgccgg
     6781 ccggaacggc atcgccgacc gcgtcaccca ccgcgtccta ggatcgctcg atatcgatcg
     6841 aagcccgccg aatgcggcgg gcttcgatcg ttagaccatc aagaacgtgg tcgccgccga
     6901 caccgtgttg ccgacctgat cgcgaagcat gacccggacc gtgtaggagc cgggggcctc
     6961 gatcgtgcgc ggcgtccacg tgacccgctg gagcccggcg acgcccgcga ttcgctcggc
     7021 gcgccagacg cgcagacccg gctggccggc gagcgtcact tcgacgtcgt agttgatcgc
     7081 gggtgaatag tcgtcggcgg cgtagaaggt gatggtgctg gcgccatcca gcggccgcac
     7141 gacgttggtc gcctgcagct gcgcgatcgt cggcgcgacg ccgtcggcgt acatgaccgc
     7201 cgacgcgagc ggcccccacg tgcccgacgc gtccctcgcc cgcacgtaga cgcggtgcgg
     7261 tccggccgcc aaggatccgg ccgtgaagtt ggcctgcaac gtctcgaacc cggaatcgaa
     7321 gacgccgtcg gcggcgccga cggggaagcc gcgatcggcg gcatcggcag gcagacggtt
     7381 gaggaacatc tcggcggccg cgaccggcgt ggcggccgtg gcgacggccg tcacggtgga
     7441 ggtgccgccg ttcgcgcgca ccggggtcgc gatcagcctc gaggtctgcg gcccggagcc
     7501 gagcccgagc gcgcggtcgg cgttaatgcg tccgtgcccg aagacgtcgt cgcggccggg
     7561 ctcgccgaga tcgtcggcgg tctgctcgat gatcgcgcgc acctgcgccg gggtgcggga
     7621 cggcacctgc gcgaacagcg cggcagcgag cccggcgacc tgaggggtgg cggcgctggt
     7681 gccgcagtcg ggcgcgacca tgaagccgtc cgaggtgatc gatgccaccg acttcgccgg
     7741 cgcgacgacc gagatcttgg gtccccagtt cgagcaggac gccttccggt cgctctggtc
     7801 ggtgccgccc acggcaagga cgttgtcgaa ccacgcggga tagaagggct ggttgttgcc
     7861 gaagttgccc gcggccgcga ccaccagcgc cggagacggc ccggtggtga cctgccggat
     7921 cgcggactcc acggtcccgt cgggcgagag caggccgagc gacaggttga tcacgcgcgc
     7981 gccgcgctgg tgcgcgtaga cgatcgcgtc ggcgagcttg gccgtctcgg cgccgccggt
     8041 gtcgctgttg accactgcga gcggcaacag gtcggcgtga tagcccacac ccacgctcgc
     8101 gaagctgttg ttcgagctcg ccgccaccag ccccgccacg aaggtgccgt gataggcggc
     8161 cggacccgag gtcggcggaa gacctgcgaa accgttcatc gacgcgatgt cgagctggcc
     8221 gccgagcgcg gcgtcctcgg aggagccgcc gttgcggaag tccacgttgg atggctgcac
     8281 ggccgtgtcg ggaacggcga cggtgacccg atgcacggcc gggatcgcca gcttggacgc
     8341 cggcggatag tagagacccg gatagcgcgc ccacgacggc tgcgccgcga tcgccgacag
     8401 atgccacacc gacgggcagg gcgggctcgc gaacgtcgag gtgcacggat cgttcggaga
     8461 tccgagctgg atgcgggcga gccggttggg ctcggcgcga acgatggcgc gctgggcgtc
     8521 gaggcgccga agcgcctgcg gcagcggccc ggcgatcgcg acgagcgcgt ggtcgcgcac
     8581 gagcagcgca cgcaccggcc gcgcgccggc gcgaccgagc gcggcagcgc gcgcggcggc
     8641 ggacgcggtg gccgcgaacc gcaccacggc ctgcccgggc acgacatcgg acgcttcgcc
     8701 gagcgcgggg cgcggcaccg cgggcgttgc ggcgacggca gcagccgccg tcaggcaggc
     8761 caccatcagg gtcgcgacga tcgcgcgccc ggcggatggt ccgcgagtct tcatggtcct
     8821 gccgttcctg ttctgcatgc gttccttcgc gtcctgtcgc tcgagcggag acgacgagcc
     8881 gttcctgcac caaacgaaac cgggcgctcg atgaagcgtc ccgatgtaag aacggcccgg
     8941 gggctcaacc cgggccgttc tcctctctcc ctcttgcctg ccggctccta ggtcgccggc
     9001 ccctcgtgga ccattccgag gccttagatg aacggcatgc acagccgtcc ggcaacgacg
     9061 ttggccagct gcaccgacgc gaaggtgatg ccggtatcgg cgatcgtcga ttgccccgag
     9121 ctgaccgtga gcggccgctg caggaaatcg atctggatgc ccgacacgtc cgcgcgcttc
     9181 ggatccgtcg tcgtctgctg cgagttgagc acgatccgca catagccgcc gttggactgc
     9241 ggatccgata tcacggtgtt cggcggcggc accacctggc tgaccttgcc ctcgttgagg
     9301 atcggcaggc cttgcgcgtt cttcaccacg acgaggtagc tgaagttgag caactccacc
     9361 cgcgtggacg gcggatcgtc gcagttggtg gacgcgcgcg ccgagatcgt gtccgaaatc
     9421 aaggtcacgt ccacgatcga gccgtccggc atcgattggc tgatcgtgcc cgagaacccg
     9481 aacagctgcg cactgctgga ggtcgtcccg ttctggccgc gcgtctggac gagcgcgccg
     9541 ccggtgaagc cgttgccggg cgaggtcgga ccgatgaagg ttccctcggt tccggttccg
     9601 gtcgggaagt cgtggtcgtg gtactggaag ttggtgtcgg cggcggtggc ctgcagaacc
     9661 gcgccggtgg agcccgcacc gacctgcccg aaggtggtca tggcggccgc gcgaccctcg
     9721 taggcccgct ccgagtcgcc catgaccacg aagtcgtcga accaggcgcg ggtgttggag
     9781 gtacgcaagc cgacggtgcc gttggcgaac atcgggcccg gcatcgaaaa tcgcgtgccg
     9841 ctgatgtcgt cgccgggtgt atcggtcacg tcgatgagga gccgtccgtc cacgtaggct
     9901 tggatgcggt tcccgctcat gacgaccttg tacacgtgcg actcggcagg gccgttggcg
     9961 ttgacgaagt tcaggaacgt cggcttcatc gtcgccagga agtaggcgcc accgatgcgc
    10021 ttgaacaggg ccacgcaccg gccggtgggg cacagctcgg gatgcgggac ctgggaggag
    10081 gtcgtgaaca ggtagaagtt gttcttgtcg gccgtgccgg tgacgggatc aaccggagcg
    10141 cggaacacga tgccgacgcc cgagttctgg ctgttgccgt cgtaggcggc acgcacctgc
    10201 acgacgaggt tctgccagtt gccgttacgc acgaacgcga ccggctcgct cgccgccgcc
    10261 ccggttgtgc gctgcttgag cgccttgatc tcgctgccgg gaccgccgat cgtcgaggtc
    10321 tgcacggagc cgaccatcca gacgttgggg atcggggtgc cgttgctcgc cgtcggcgtg
    10381 atctgccact ggagctgatc gatcggcacg tcgttggtcg gcgtgacgcc ggcgtagtcg
    10441 aagtgatcgg aatagccgtt caccagcccg ccacgaatat cggcgatgcc ggggttgagc
    10501 ggagcgatcg ccaccgcaac gcaaagcatc gccagcgcga cacgccatct ggtccacgag
    10561 ccgaacgtca tcgaaacctc ctcgttgcac cggctgagcc tccggcgcac ggggaatcat
    10621 ttcgcggccg ccggccgtgc tcctgcggat cccgcgcacg attagcgggg caatgcggcg
    10681 gcgcttcgca gccgtgactt tgggcgcatc gggggcgttg gcatccaccg cgggaccaaa
    10741 gcgaacccca tttgccgcgg cccggggcga aacgaaaccg caccacgagc gaggaacgtt
    10801 cggcgcatcg cggcgaaagc gaaacgaggc tctacggcat cagttcggga ggcggcggcc
    10861 cgaagcggcc gagcaggccg tgcctcatgc cgagcatgat ggcgcggcgc agcggcggcg
    10921 ggctcttgcg cgcccagtcg gcgtagcggc gcgtcgcgcg cgcctcggcg agccagcggg
    10981 ccggcggggc cagatgcgta cgcagaagca ggtacgggtt ccggacgaag aagtaccgct
    11041 gaagcgggg
//
LOCUS       DATLFO010000099         4290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_22844, whole genome shotgun sequence.
ACCESSION   DATLFO010000099 DATLFO010000000
VERSION     DATLFO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22844"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VM841_03395"
     CDS             <1..672
                     /locus_tag="VM841_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89262.1"
                     /translation="AYVFGGSIDIPGSSYREFLIAGIFAQTIVFGSTFTGAGLADDMQ
                     KGIIDRFRSLPMARSAVLIGRTASDIFTNSVSIIVMSVTGLIVGWRIRTSVPEAIVGF
                     ALLLIFAYAVSWIMAWVGLKVKSVEVVNNASFMFIFPMTFIANTFVPSENLPLVLRTF
                     AEWNPVSAVTQAVREAFGNTTGLAPASTWSLRNPEIYTLLWVAIILAVFVPLAVRQYR
                     IAAVR"
     gene            complement(737..1489)
                     /locus_tag="VM841_03400"
     CDS             complement(737..1489)
                     /locus_tag="VM841_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89263.1"
                     /translation="MAGPSVRLVRAGASDPETLGSMTAFADGALIGPSIVGTIVAIPM
                     HDGTREATSVELRDIVAGSTTTLTIPAGQRYVGHTGDGGILVDVTTETWTGTEYAKDL
                     SLRAADGTLTRLMDVRARTNAVLADDAGILVTQYRFEGGGVNLGVTSAIRTVYVASKI
                     SASPSSATIRRGSSVRIAGNVWPAHAGQLVALQHLTGSGWRTVTWAKLTSTGYAFAVR
                     PASRGTFRYRIYKPADADHAGAVGPTLTITVR"
     gene            complement(1563..1946)
                     /locus_tag="VM841_03405"
     CDS             complement(1563..1946)
                     /locus_tag="VM841_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HVL89264.1"
                     /translation="MSDLRTEPQPGRTGRASVTVNEDLTAIALGSGDVPVYGTPAVLA
                     LLEAAAVDALAGALPDGKTSVGVQVTLDHLAASKVGATVDAVATLDSVDGRRLSFTCE
                     AREGDTVIARATHRRVIVDRASFGA"
     gene            1994..2767
                     /locus_tag="VM841_03410"
     CDS             1994..2767
                     /locus_tag="VM841_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89265.1"
                     /translation="MNGPAGIMALVLAETAVGGAAVLALTPLRGRVRHAFYKLVGAVL
                     GTTAVLAWLSARAPLGGFEATGSRAAAFWLLGLTATIAVVWQVLLWTSEKLSFYAGYA
                     MIPAGVASFAALAALPGARSNAVLGFLQLSGGAFFLGAVTVGLLLGHWYLVDRKLGPE
                     PLGRVGQYLLAGSIVAAIMAIAGGGGGASATTSLSPLLGAGVLAVAIAVGLAALCTMI
                     AFFIRALVKEGSMQAATGFFYLAVLMGLSAEFAAKIRFF"
     gene            complement(2764..3036)
                     /locus_tag="VM841_03415"
     CDS             complement(2764..3036)
                     /locus_tag="VM841_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89266.1"
                     /translation="MSEPEQRHQDQPQRKFTKAEAKLVAFGVLALLAGVFVGQNTGQV
                     RVHFVFVSANIRLIWVFLLCILIGVSLDRLLTRRGVLPPSKNKRER"
     gene            3147..>4290
                     /locus_tag="VM841_03420"
     CDS             3147..>4290
                     /locus_tag="VM841_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVL89267.1"
                     /translation="MEPTATSVEQPDAGQARPAEPPAGPWHSTAEPAAPWRRARTSPW
                     SEPAGETAAAPAEETPPLGPWAVPEPALSQDEASALPAWPAPLPGIPSTSEPPPEGTA
                     RSRPGRPFIVFLSALLGAVLGVGSTIVVLDRAGVGGIGGGEVRVVDPPVQGGGTANLD
                     GVAAVAKAVSPSIVRIDTRARSAFGSQSGTGSGVIYRTDGYIITNAHVVDGATSVEVT
                     LSTGDTLQGSVVGTAAPIDDIAVVKVNRDKLPAATLGSVLNTNVGDTAIAIGSPFGLS
                     GTVTAGIVSAIRANLSLGNQQFVDVIQTDAPINPGNSGGALVNSRGEVVGINTAILGG
                     EANVGIGFAIPIDIARKEADDIIRIGRATRAFLGVEPATVPGRNGAR"
BASE COUNT          590 a         1535 c         1468 g          697 t
ORIGIN      
        1 gcctacgtgt tcggcggctc gatcgacatc ccgggaagct cgtaccggga gttcctgatc
       61 gccggcatct tcgcgcagac gatcgtgttc ggatcgacgt tcaccggcgc gggtctggcc
      121 gacgacatgc aaaagggcat catcgatcgg ttccggtcgc tgccgatggc gcggtcggcc
      181 gtgctgatcg gccgcaccgc cagcgacatc ttcaccaact cggtgtcgat catcgtgatg
      241 tcggtcaccg gtctgatcgt cggctggcgg atccgcacct cggtgcccga ggcgatcgtc
      301 gggttcgcgc tgctgctgat cttcgcctac gcggtgtcct ggatcatggc gtgggtcggc
      361 ctgaaggtga aaagcgtcga ggtcgtcaac aacgcctcgt tcatgttcat cttcccgatg
      421 accttcatcg ccaacacgtt cgtgcccagc gagaacttgc cgttggtgct tcgcacgttc
      481 gcggagtgga acccggtgtc ggccgtgacc caagcggtgc gggaagcgtt cggcaacacg
      541 accgggctgg ctcccgcgtc gacctggtcg ctgcgcaacc ccgagatcta cacgctgctg
      601 tgggtcgcga tcatcctggc cgtgttcgtt ccgctcgcgg tccggcaata ccggatcgcc
      661 gccgtccgat aggcggcggc gacgccgccg ggcgcgggct cggatcggag ccttgcccgg
      721 cgacgtcact ccgtcgctat cgcaccgtga tcgtcagcgt tgggccgacc gcgccggcgt
      781 ggtcggcgtc ggcgggcttg tagatgcggt agcggaacgt tcctcgcgat gcgggccgca
      841 ccgcgaacgc gtagccggtc gaggttagct tcgcccacgt gacggtccgc cagccggaac
      901 ccgtcaggtg ctggagcgcc acgagctggc cggcgtgcgc aggccagacg ttgccggcga
      961 tccgcacgga cgagccgcgc cggatcgtgg ccgacgacgg cgacgccgag atcttgctcg
     1021 cgacgtagac ggtccggatc gcgctcgtca cgccgaggtt cacgccgccg ccctcgaagc
     1081 ggtactgagt gaccaggatg cccgcgtcgt cggccaggac ggcattcgtg cgcgcgcgca
     1141 cgtccatcag gcgggtcagc gtgccgtcgg cggcccgcag ggaaagatcc ttggcgtatt
     1201 cggttccggt ccacgtctcg gtcgtgacgt cgaccaggat gccgccgtca ccggtgtgcc
     1261 cgacgtagcg ctgtcccgcc ggaatggtga gcgtggtcgt cgagcccgcg acgatgtcgc
     1321 gcagctcgac cgaggtggcc tcgcgcgttc cgtcgtgcat cgggatcgcc acgatggttc
     1381 ccacgatcga gggcccgatc agtgctccat cggcgaaggc ggtcatcgag ccgagggtct
     1441 cgggatccga ggcgccggcg cgaaccaggc gcaccgacgg accggccatg taggagccac
     1501 aatgctcgct tgtgcgcggc agcgccgcag gaggacaacg aggccggtga cgcggcgttt
     1561 cgctaggcgc cgaacgaggc gcgatcgacg atcacgcgtc gatgcgtcgc gcgcgcaatc
     1621 acggtgtcgc cctcgcgcgc ttcgcaggtg aacgacaacc tccgcccgtc cacgctgtcc
     1681 agcgtcgcga cggcgtccac ggtggcaccg accttcgaag cggcgaggtg gtccagcgtc
     1741 acctgcaccc cgaccgaggt ctttccgtcg ggcagcgcgc cggcgagcgc gtccaccgct
     1801 gcggcctcga gcaaggccag cactgccgga gttccgtaga ccggcacgtc gcccgatccc
     1861 agcgcgatcg cggtcaggtc ctcgttcacg gtgacgctcg cgcgcccggt tcgcccgggt
     1921 tgtggctcgg ttcgaaggtc gctcatggcg cgcctataat ggccgccgcg cgctccccgg
     1981 cgcgaatcca tccatgaacg gacctgctgg aatcatggcg ctcgtcctcg ccgagacggc
     2041 ggtgggcggc gctgccgtgc tcgcgctcac gccgctgcgt gggcgggtcc ggcacgcctt
     2101 ctacaagctc gtcggcgcgg tgctcggcac caccgcggtg ctcgcttggc tgtccgcccg
     2161 cgcgccgctc ggcggcttcg aggcgaccgg ctcgcgcgcc gcggcgttct ggctgctcgg
     2221 cctcaccgcc acgatcgccg tcgtgtggca ggtgctgctg tggaccagcg agaaactcag
     2281 cttctacgcc ggatacgcga tgatccccgc aggcgtcgcc tcgttcgcgg cgctggccgc
     2341 gctgccgggc gcgcgcagca acgccgtgct cggcttcctg cagctgtccg gcggcgcgtt
     2401 cttcctcggc gccgtcaccg tcgggctgct gctcggccac tggtatctgg ttgaccgcaa
     2461 gctcggtccc gagccgctcg gtcgcgtcgg gcaatacctg ctcgccggat ccatcgtcgc
     2521 ggcgatcatg gcgatcgccg gcggcggcgg gggagcgagc gccaccacgt cgctgtcgcc
     2581 gctgctgggc gccggcgtgc tcgcggtcgc gatcgcggtc ggcctggccg cgctgtgcac
     2641 gatgatcgcc ttcttcatcc gcgcgctcgt caaggaaggc tctatgcagg ccgcgaccgg
     2701 cttcttctac ctcgccgtgc tgatgggcct gtcggccgag ttcgcggcca agatccgttt
     2761 cttctaacgc tcgcgcttgt tcttcgacgg cggcagcacg cctcgccgcg tcagcagccg
     2821 gtccagcgac accccgatca ggatgcacag caggaacacc caaatcaggc ggatgttggc
     2881 ggatacgaac acgaagtgga cgcgcacctg accggtgttc tggccgacga acacgcctgc
     2941 caggagcgcg agcaccccga aggcgaccag cttggcctcg gccttggtga acttgcgctg
     3001 cggctggtcc tgatgtcgct gttcgggctc gctcatgcct tgtattctca cacgaaatga
     3061 gctctcatcc ttcgcttggg gaaccttcag ggggctctca ggggcgtccc cctagactcc
     3121 cttccgagaa cccggaagga ggggagatgg aacccacggc gacctctgtc gagcagcccg
     3181 acgccggcca ggcccggccc gccgagccgc ccgcaggacc gtggcattcc acggccgagc
     3241 ccgccgcgcc gtggcgccgc gctcgcacga gcccctggag cgagcccgcc ggcgagacgg
     3301 ccgccgcacc cgcggaggag acaccgccgc tcgggccgtg ggcggttccc gagccggccc
     3361 tgtcgcagga cgaggcttcg gcgctgcccg catggcccgc cccgcttccc gggatccctt
     3421 cgacttcgga gccccctccg gaagggacgg cgcggagccg cccgggccgt cccttcattg
     3481 tttttctttc cgcgctcctc ggcgccgtcc tcggcgtcgg ctcgacgatc gtggtgctcg
     3541 accgcgccgg cgtcggcggc atcgggggtg gcgaggtccg cgtcgtcgat cctcccgtgc
     3601 agggcggcgg gaccgccaac ctcgacggcg tcgcggccgt cgccaaggcc gtctcgccga
     3661 gcatcgtgcg catcgacacg cgcgcgcggt cggcgttcgg ctcgcagtcc ggcaccggct
     3721 caggcgtgat ctaccgcacc gacggctaca tcatcaccaa cgcgcacgtc gtcgacggcg
     3781 cgaccagcgt cgaggtcacc ctctcgaccg gcgacacgct gcaaggcagc gtcgtgggga
     3841 ccgcggcgcc gatcgacgac atcgccgtgg tcaaggtcaa ccgcgacaag ctgccggccg
     3901 cgacgctcgg ctcggtgctc aataccaacg tcggtgatac cgccatcgcg atcggctcgc
     3961 cgttcggtct ttccggcacg gtcaccgcgg gaatcgtcag cgcgatccgc gcaaacctct
     4021 cgctcggaaa ccagcagttc gtcgacgtga tccagaccga cgcgccgatc aaccccggca
     4081 actccggcgg cgcgctggtg aactcgcgcg gtgaggttgt cggcatcaac accgcgatcc
     4141 tgggcgggga ggccaacgtc ggcatcggct tcgcgattcc gatcgacatc gctcgcaagg
     4201 aggccgacga catcatccga atcggccgcg ccacgcgcgc gttcctcgga gtcgagccgg
     4261 cgacggtgcc cggccgcaac ggcgcgcgga
//
LOCUS       DATLFO010000100         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_102817, whole genome shotgun sequence.
ACCESSION   DATLFO010000100 DATLFO010000000
VERSION     DATLFO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102817"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VM841_03425"
     CDS             complement(<1..221)
                     /locus_tag="VM841_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89268.1"
                     /translation="MTGRRRSRWIVRILALVSLASTLPANAAGPTVRSAPYDFGCFGD
                     SGSPSSCVPWPGARETGLVQAGSAFGVYPS"
     gene            complement(323..1210)
                     /locus_tag="VM841_03430"
     CDS             complement(323..1210)
                     /locus_tag="VM841_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVL89269.1"
                     /translation="MDAEDLAAVRAIVRAGTFSAAAEDLRIAQPALSRRIAKLEKELG
                     GLLFDRLPRHAAPTPLGLEIASAASRVLAELDRAREAAEAITSGAAGRLRLSSLAGGI
                     AVLARGLERFHLAHPQVDVEIRSLDAEEAIRALHDREVDLATLAGSALEEGMEFRKLA
                     TWRVVLTVPPEHRFASMASVTIGALAAEPLLMLAPEFMVTRHVTHLAERAGLRLRVRL
                     RDATPEAVVSLARRGLGVGVLPDSVRLPPDLRSVPLARAGSSADFDYVVAWLAERTLP
                     AAAESLVEFLVRETAAFRA"
     gene            1328..2275
                     /locus_tag="VM841_03435"
     CDS             1328..2275
                     /locus_tag="VM841_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVL89270.1"
                     /translation="MRARRSCLSVPGSSAKFLAKAATLDADEVFFDLEDSVAPLEKEA
                     ARGNVVQALKEHEYKARTKVLRVNGVYTKWCAKDLLEVVENAGEQLDCIMIPKVETVA
                     DVKFVSNMLRMIEDNMGMEKRIGIEVQIETALGLLNLPQIAACDDRIEDLILGPADMS
                     ASLGLPTVTAGEPIPGYETLDHFHYVLTHINIAARANNLQAIGGPYLLIRDLDGFRAA
                     AMRERALGYDGKWALHPDQVAILNEVFTPTQDEFDKSEAVLEHYKYVTEVERKGAVMF
                     GKEMIDEASRKMAEQFAARGRAAGMKVVKTAKDFLGDAK"
BASE COUNT          405 a          786 c          784 g          304 t
ORIGIN      
        1 gatgggtaca cgccaaaagc agatcccgcc tgaacgaggc cggtctcgcg ggcgccgggc
       61 cacgggacgc acgacgacgg cgagccggag tcgccgaagc acccgaagtc gtacggagcg
      121 gatcgcacgg tgggcccggc cgcgttcgcg ggcagggtgg acgccaggct cacgagcgcc
      181 aggatgcgca cgatccatcg tgagcgacga cgaccggtca tcggtcgcgg agcggtcggc
      241 acgtgactcc cccggacgtg attgcctatc cgtttaaggg tagatcgtcg gcgcctcggc
      301 gacaaggggt tcccggccgc agttaggcgc ggaacgcggc ggtctctcgc accaggaact
      361 cgaccagcga ctcggccgcg gcgggcaggg tgcgttccgc gagccaagcg acgacgtagt
      421 cgaagtcggc gctggatccg gcgcgggcga gcggaacgga ccgcaggtcc ggcggcagcc
      481 ggaccgagtc gggcagcacg ccgacgccca agccgcggcg cgcgagcgac acgaccgctt
      541 cgggtgtggc gtcccgcagc cggacccgca gccgcaggcc ggcgcgctcg gccagatgcg
      601 tgacgtgccg cgtgaccatg aactcgggcg cgagcatcag cagcggctcg gccgccagcg
      661 ccccgatcgt caccgacgcc atcgacgcga accgatgctc cggcgggacc gtcagcacca
      721 cccgccaggt cgcgagcttc cggaactcca tgccttcctc cagcgcgctg cccgccagcg
      781 ttgccagatc gacttcgcgg tcgtgcagcg cgcggatcgc ctcttcggcg tccagcgacc
      841 ggatctcgac gtccacctgc ggatgcgcga gatggaaacg ctcgagcccg cgggccagga
      901 ccgcgatccc gcccgccagc gatgacagcc ggagccgtcc ggcggctccg ctggtgatcg
      961 cctcggccgc ttcgcgggct cggtccagct cggccagtac gcgagaggcg gcggacgcga
     1021 tctccaggcc cagcggggtg ggggcggcgt ggcgagggag gcggtcgaac agcagcccgc
     1081 ccagctcttt ctccagcttc gcgatcctgc gcgacagggc cggctgggcg atgcggaggt
     1141 cctcggccgc ggcagagaag gtacccgccc gcacgatggc gcggaccgcg gccaagtcct
     1201 cggcatccat ccgggcagaa ctataacaga taggcatggt tttgccaccg tacggttatt
     1261 ggacagcatg aaagcggcgg cgggatactg gccccgtcaa cccgcgatca ccaggaggtc
     1321 cacccgaatg cgcgcacgtc gctcttgcct gtcggtcccc ggctcgtcgg cgaagttcct
     1381 cgccaaggcc gcgaccctgg atgccgacga ggttttcttc gacctcgaag actcggtcgc
     1441 tccgctcgag aaggaagccg cgcgcgggaa cgtcgtgcag gctctcaagg agcacgagta
     1501 caaggcccgc accaaggtgc tgcgcgtcaa cggcgtctac accaagtggt gcgccaagga
     1561 tctgctcgaa gtcgtcgaga acgccggcga gcagctggac tgcatcatga tccccaaggt
     1621 cgagaccgtc gccgacgtga aattcgtgtc gaacatgctt cgcatgatcg aagacaacat
     1681 gggcatggaa aagcggatcg gcatcgaggt gcagatcgag accgcgctgg gcctgctgaa
     1741 ccttccccag atcgccgcat gcgacgatcg catcgaggac ctgatcctcg gcccggccga
     1801 catgagcgcc tcgctgggtc tgcccaccgt gaccgcgggt gagccgatcc ccggctacga
     1861 gacgctcgac cacttccact acgtgctgac ccacatcaac atcgccgcgc gcgccaacaa
     1921 cctgcaggcg atcggcggcc cgtacctgct catccgcgat ctggacggct tccgtgccgc
     1981 cgcgatgcgc gagcgcgcgc tcggctacga cggcaagtgg gcgctgcacc ccgatcaggt
     2041 cgcgatcctc aacgaggtct tcaccccgac ccaggacgag ttcgacaagt ccgaggcggt
     2101 gctcgagcac tacaagtacg tcaccgaggt cgagcgcaag ggcgcggtga tgttcggcaa
     2161 ggagatgatc gacgaggcga gccgcaagat ggccgagcag ttcgccgcgc gcggccgcgc
     2221 cgccggcatg aaggtcgtca agaccgccaa ggacttcctg ggcgacgcca agtaaggag
//
LOCUS       DATLFO010000101         7328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_52831, whole genome shotgun sequence.
ACCESSION   DATLFO010000101 DATLFO010000000
VERSION     DATLFO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52831"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..883
                     /locus_tag="VM841_03440"
     CDS             104..883
                     /locus_tag="VM841_03440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL89271.1"
                     /translation="MPDGEVSNWKIPTGGDAAFSAYGPLVVLPSGTLMQTIYGWGKGY
                     YRIWGIYSFDDGYRWNYGREIWRTSVAMPNETAAAYVDGRTDWTSRLIAVHRSARRVD
                     GRWFGYIMQSVSTDGGRTWRQAGVIGKETRRGVIPWIANIGKGRLALLTAVRDHDDLS
                     INVSIAPAWKVVSNPRAWPEPRTIYRSRLMDTERIKIGSIEQSKNFGDFGYQSIVRTG
                     PTDADLFVVLMDSHNGHSGFKPDDYPSNTELVMIPLFGETL"
     gene            complement(880..1215)
                     /locus_tag="VM841_03445"
     CDS             complement(880..1215)
                     /locus_tag="VM841_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89272.1"
                     /translation="MSDDFETDRADFIRRSGSDFSGYSRADVPFFHPASVRRGRGGVP
                     APVKKPTGVAQLSVALILLFGAVAFFLTFDSLLPRIVLLFELALPVLIIVAVVSVRRK
                     NRRRLEEMD"
     gene            complement(1263..1985)
                     /locus_tag="VM841_03450"
     CDS             complement(1263..1985)
                     /locus_tag="VM841_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL89273.1"
                     /translation="MFYWLMKHVFIGPLVRALYRPWIEGLENVPKDGPVILAANHNSF
                     LDSIFLPLVVPRNVVFLGKSDYFDKWYMKWFFKGANVIPVRREGGEASEAALNAGVGA
                     LRQGLAVGIYPEGTRSPDGRLYRGKTGVARMALRVGCPIVPVAIIGSREAQPPDRKMP
                     KICRVGLRFGRPMDLSRYAGKDDDRFVLRSVTDELMFEIMMLSGQEYVDEYAAKYKTQ
                     KAADEAKRPPSAGSQPPLRAVS"
     gene            2162..2923
                     /locus_tag="VM841_03455"
     CDS             2162..2923
                     /locus_tag="VM841_03455"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL89274.1"
                     /translation="MSSKPAVMPGAEPFAFDGGTTGVLLVHGFTGTPQGLREWGEYLA
                     GQGYAVICPRLPGHGTQPRDLHDVTFREFVEEAERGLDGLLQRCQSVFIAGLSMGGTI
                     VMDIVQRRGDELAGMILVNPFLYTPDPRAKLTPVLGKLPLLLKGVYNDIAEPGQNELG
                     YPKLSTKAFASLWSAAKGIKTRLPSVHLPTLIFASRQDHVVHPGNASLVHESISSTDK
                     ELVWLDRSFHVATLDYDRHLIFERSAKFVASHEET"
     gene            2920..3240
                     /locus_tag="VM841_03460"
     CDS             2920..3240
                     /locus_tag="VM841_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89275.1"
                     /translation="MNDTLRTAYLGRYDPLVIPILLDMLRDEGIYSTTKLPHDQPARG
                     EYAMFSSGADQVLVDASRLDEARRLVDERLPQILAEMSSGLDAEFGPEPEFEPGPEPD
                     GRTP"
     gene            3237..3947
                     /locus_tag="VM841_03465"
     CDS             3237..3947
                     /locus_tag="VM841_03465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVL89276.1"
                     /translation="MSVFAGTRAVTFDCWGTLIYESDFVRGRELRVAAVGELTGLGAE
                     QAGELLQRAWQRHHDEWTSERQHGAEGIARDICAELGAGPEVETELVRKLEEASLQCE
                     VAMVPGAAETLRTIKRAGLRTALICDTGFSPGRVVRELLGRHGLTDHLDHLVFSDEVG
                     VPKPNHKMFAAALDAVGGGPAVHIGDLRRTDVAGARAAGLHSVRFRGIYDDLGEHPDA
                     DHVVDELSHLLQLLGLSS"
     gene            3950..5158
                     /locus_tag="VM841_03470"
     CDS             3950..5158
                     /locus_tag="VM841_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HVL89277.1"
                     /translation="MQTEVLELRGHIIDSLTLPKVLDEIVESGASYRILTFEIGHTHD
                     DPSFCSIEITGEPASVAALLPRLQDLGANPPEIGDVTLVAADGDGVFPEGFYSTTNLP
                     TKVRVDGEWIAVAPSEMDCGIVVNRSAKSARTVPVSEIARGDLVVCGHGGVKVSPLER
                     KRDGQTFSFMSSEVSSEKPQGLMVDRVAALAREIRASGKKILWVLGPAVVHTGTIESF
                     TALIDGGWVDVVFAGNALATHDCEAAIYGTSLGVSLTEGSAAEHGHEHHIRAINTVRR
                     AGSFKAAVEAGVLTSGIMYSLITHGVDYVLAGSIRDDGPMPEVITDVLEAQRQMRART
                     EGLGLAMMTGTMLHSIATGNLLPASVPIVCVDINPAAVTKLLDRGSIQSVGIVTDVGL
                     FLRRLADTLG"
     gene            complement(5155..5787)
                     /locus_tag="VM841_03475"
     CDS             complement(5155..5787)
                     /locus_tag="VM841_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVL89278.1"
                     /translation="MTLRVVTYNVQGGVRGVEAVAGVLRDLAPDIACLNEVRGANLRP
                     IARIAGRRAVFGPTIGVRRFGNAILAREPFGRVTRIRLSKSPGMERRGLLIADCGGVT
                     VAATHLGHAGEERARHLEEILGHVGQARPAILAGDFNEPADGAALRRLAGHFTDAFAV
                     AGEGGGATFPSNGPDRRIDYVFARGIDVVSCEVPRVSASDHLPVVAMLEP"
     gene            complement(5784..6701)
                     /locus_tag="VM841_03480"
     CDS             complement(5784..6701)
                     /locus_tag="VM841_03480"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HVL89279.1"
                     /translation="MRAVVALGGNAIVRHGQRGTVAQQRAAIAQAASGLGVVASRGIE
                     LVVTHGNGPQVGRLLLQNLAAQRRIPMMPLDVLGAESQAEIGYMLQQALSATLSPRPV
                     ATVITQTVVAAGDPAFADPTKPVGPSMLAPAARALEARGIAVARDEMRGGWRRVVASP
                     RPLRLVEEAAIRALADAGITPIAAGGGGVPVIEEDGGYRGVDGVVDKDLAAAILARAT
                     EAAMMVILTDVEHVIVDRGTRGERSVGRMTVAEARERLEAGEFPAGSMGPKVEAVVQA
                     AEEGRRAIITSLESVADAIEGTAGTEVVA"
     gene            complement(6706..>7328)
                     /locus_tag="VM841_03485"
     CDS             complement(6706..>7328)
                     /locus_tag="VM841_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVL89280.1"
                     /translation="YTPDVHITVVDPLRAGHENRYHPGEANVRAANVLVINKIDSATA
                     EQVAEIEASIRELNPGATVVRAHSPVTVDHPDAIKGKRVLVVEDGPTLTHGEMKYGAG
                     VVAARNAGAAEIVDPREHAVGSIRETFASYPEIGTLLPAMGYGKQQMSDLAATIDATP
                     ADVVVIATPVDLRRVAEFSKPAVRVTYDLEEHGSPTLADVLAPLTR"
BASE COUNT         1147 a         2572 c         2524 g         1085 t
ORIGIN      
        1 ccagatctcg cggccgtaat tccaccggta gccgtcgtcg aacgaataga tgccccagat
       61 gcggtagtag cccttgcccc agccgtagat cgtctgcatc agcgtgccgg acggcgaggt
      121 ctcgaactgg aagatcccca ccggcggcga cgcggcgttc tcggcctacg gcccgctggt
      181 ggtgctgccg tccggcacgc tgatgcagac gatctacggc tggggcaagg gctactaccg
      241 catctggggc atctattcgt tcgacgacgg ctaccggtgg aattacggcc gcgagatctg
      301 gcgcaccagc gtcgcgatgc ccaacgagac cgccgccgcc tacgtggacg gccgcaccga
      361 ctggacctcg cggctgatcg cagtccaccg gtcggcccgg cgcgtggacg gccgatggtt
      421 cggctacatc atgcagtcgg tttcgaccga cggcggacgg acgtggcggc aggccggggt
      481 gatcggcaag gagacccgcc gcggcgtgat cccgtggatc gccaacatcg gcaagggccg
      541 gctggcgctg ctgaccgcgg tgcgcgatca cgacgacctg agcatcaacg tcagcatcgc
      601 gcccgcatgg aaggtcgtgt cgaacccgcg cgcctggccg gagcctcgca cgatctaccg
      661 cagccggctc atggacaccg agcgaatcaa gatcggatcg atcgagcagt cgaagaactt
      721 cggggacttc ggctatcagt cgatcgtacg gacggggccg accgacgccg atttgttcgt
      781 ggtgctgatg gactcgcaca acgggcactc gggcttcaag cccgacgact atcccagcaa
      841 caccgagctg gtgatgatcc cgctgttcgg cgagacgctc taatccatct cttcgagccg
      901 gcggcggttc ttgcgccgca ccgacaccac cgccacgatg atcagcaccg gcagcgccag
      961 ctcgaacagc agcacgatcc ggggcagcag cgagtcgaag gtcagaaaga acgcgaccgc
     1021 tccgaacagc aggatcagcg cgacggagag ctgggcgacg cccgtgggct tcttgaccgg
     1081 ggcgggcacg ccgccgcggc cgcgccgcac cgacgcagga tggaagaacg ggacgtcggc
     1141 ccgcgaatag cccgagaaat cgctgccgga acgccggatg aagtcggcgc ggtcggtttc
     1201 gaagtcgtcg ctcacgaacg acagcgtacg cccgcggggg ccgtggggaa agagagacgc
     1261 ggctaggaga cggcgcggag cgggggctgc gagccggccg acgggggacg cttggcctcg
     1321 tcggcggcct tctgcgtctt gtacttggcc gcgtactcgt ccacatactc ctggcccgac
     1381 agcatcatga tctcgaacat cagctcgtcg gtcaccgagc gaagcacgaa ccgatcgtcg
     1441 tccttgcccg cgtagcggga caggtccatc gggcggccga agcgcaagcc gacgcgacag
     1501 atcttgggca tcttgcggtc cggcggctgc gcctcgcgcg agccgatgat ggcgaccggc
     1561 acgatcgggc agcccacgcg cagcgccatg cgcgccacgc cggtctttcc gcgatacagc
     1621 cgcccgtcgg gcgagcgggt gccctcggga tagatgccga cggccaggcc ctgccggagc
     1681 gcgccgacgc ccgcgttgag cgcggcctcg ctcgcctcgc cgccctctcg ccggaccggg
     1741 atgacgttgg cgcccttgaa gaaccacttc atgtaccact tgtcgaagta gtcgctcttg
     1801 ccgaggaaga ccacgttccg cggaaccacc agcggcagga agatgctgtc gaggaaggag
     1861 ttatggttgg cggccaggat gaccggcccg tccttcggca cgttctcgag cccttcgatc
     1921 cacgggcgat acagggcacg caccagcggc cctataaaaa cgtgcttcat cagccagtag
     1981 aacacaggat ccggctccca acggctccgt gcttctcccg gcacgatact ccggcgcggg
     2041 gaagcgcaca atggactgcg accgcctgtt agcctagcag cgtcaccggg gcgctgtcgg
     2101 ccccgaatcg cggtaatttt ggcgacgtat cgtcgtcaag gcgttcagtc gggaggaagc
     2161 attgagcagc aagccggccg tcatgcccgg agccgagccg ttcgccttcg acggcggcac
     2221 gaccggggtc ctgctcgtcc acggcttcac cggcacaccg cagggcctcc gagagtgggg
     2281 ggagtacctg gccggacagg gctacgccgt gatctgtccc cgcctgcccg ggcacggcac
     2341 ccagccgcgc gacctgcacg acgtcacgtt ccgtgagttc gtcgaagagg ccgagcgcgg
     2401 cctcgacggc ttgctgcagc gctgccagag cgtcttcatc gccggcctgt cgatgggcgg
     2461 cacgatcgtg atggacatcg tgcagcgtcg cggcgacgag ctggcgggca tgatcctggt
     2521 caacccgttc ctgtacacgc ccgacccgcg cgcgaagctc accccggtcc tgggcaagct
     2581 gccgctgctg ctcaagggcg tctacaacga catcgccgag cccggacaaa acgagctcgg
     2641 ctacccgaag ctgtccacga aggctttcgc ctcgttgtgg tcggcggcca aggggatcaa
     2701 gacccgcctg ccgtccgtgc acctgccgac gctgatcttc gcgtcgcggc aggatcatgt
     2761 cgtccacccc ggcaacgcct cactcgtgca cgagtcgatc tcctcgaccg acaaggagct
     2821 ggtgtggctg gaccggtcct ttcacgtggc cacgctcgac tacgaccggc acctgatctt
     2881 cgagcggtcc gccaagttcg tcgcgagcca cgaggagaca tgaacgacac gctgcgcacc
     2941 gcctacctgg gacgctacga cccgctcgtg atcccgatcc tgctggacat gctgcgcgac
     3001 gagggcatct actcgacgac gaagctgccg cacgaccagc cggccagggg cgagtacgcg
     3061 atgttctcgt ccggggccga tcaggtgctg gtggatgcct cccggctcga cgaggcgcgg
     3121 cggctcgtgg acgagcgact gccacagatc ctggccgaga tgagctccgg cctcgacgcc
     3181 gagttcggcc ccgagccaga gttcgagccc ggccccgagc ccgacggacg gacgccgtga
     3241 gcgtgttcgc cggtacccgc gccgtgacct tcgactgctg gggcacactg atctacgaat
     3301 ccgacttcgt gcgcggccgc gagcttcgcg tcgccgccgt cggcgagctg accggcctgg
     3361 gcgccgagca agccggcgag cttcttcagc gcgcgtggca acggcaccac gacgagtgga
     3421 cctccgagcg ccagcacggc gccgagggca tcgcccgcga catctgcgcc gagctcggcg
     3481 ccggccccga ggtcgagacc gagctggtcc gcaagctcga ggaggcctcc ttgcagtgcg
     3541 aggtggcgat ggtaccgggc gccgccgaga ccctccgcac gatcaagcgc gccggcctcc
     3601 ggaccgcgct gatttgcgac accggcttct cgccgggccg cgtcgtgcgg gagctgctcg
     3661 gccgtcacgg gctcaccgat catctggatc atctcgtgtt cagcgacgag gtcggcgtgc
     3721 ccaagccgaa ccacaagatg ttcgccgccg cgctcgacgc ggtcggcggc ggacccgcgg
     3781 tgcacatcgg ggatcttcgc cgcaccgacg tggcgggcgc gcgcgccgcg ggcctgcaca
     3841 gcgttcgctt ccgcggcatc tacgacgacc tgggcgagca tcccgacgcc gaccacgtcg
     3901 tggacgagct gtcgcatctt ctgcagctgc tcgggctatc gtcctagcca tgcagaccga
     3961 ggttctggag cttcgcggcc acatcatcga ctcgctgacg ctgcccaagg tgctcgacga
     4021 gatcgtcgag tcgggcgcgt cttaccggat cctgaccttc gagatcggcc acacgcacga
     4081 cgatccgtcg ttctgctcga tcgagatcac cggcgagccc gcctcggtcg cggccctgct
     4141 gccgcggctg caggacctgg gcgcgaaccc gcccgagatc ggcgacgtca cgctcgtggc
     4201 cgcggacggc gacggcgtgt tcccggaagg cttctattcg accacgaacc tgcccacgaa
     4261 ggtgcgtgtc gacggggaat ggatcgcggt cgcgccctcg gagatggact gcggcatcgt
     4321 ggtgaaccgc agcgcgaagt ccgcgcggac cgttccggtc agcgagatcg cgcgcgggga
     4381 tctggtggtg tgcggccacg gcggggtcaa ggtctcgccg ctggagcgca aacgtgacgg
     4441 ccagactttt tcgttcatgt cctcggaggt gtcctcggag aagccacaag gcttgatggt
     4501 cgatcgcgtg gccgccctcg cgcgcgaaat cagggcctcc ggcaagaaga tcctgtgggt
     4561 gctcggaccc gccgtcgttc ataccggcac gatcgagtcc ttcaccgcgc tgatcgacgg
     4621 cggctgggtg gacgtggtct tcgccgggaa cgcgctcgcc acccacgact gcgaagccgc
     4681 catctacgga acgtcgctgg gcgtgtcgct gaccgaggga agcgccgccg agcacggcca
     4741 cgagcaccac atccgcgcga tcaacacggt tcgccgcgcc ggctcgttca aggccgcggt
     4801 ggaggcgggt gtgctcacga gcgggatcat gtactcgctg atcacccacg gcgtggacta
     4861 cgtcctggcg ggttcgatcc gcgacgacgg cccgatgccc gaggtgatca ccgacgtgct
     4921 ggaagcgcag cggcagatgc gggcccgcac cgaggggctg gggctggcga tgatgaccgg
     4981 caccatgctg cactcgatcg cgacgggcaa cctgctgccg gcctccgtgc cgatcgtgtg
     5041 cgtggacatc aacccggcgg ccgtcaccaa actgctcgac cggggctcga tccagtcggt
     5101 ggggatcgtc accgacgtcg ggctgttcct gcgccgcctg gccgatacgc tcggctaggg
     5161 ctcgagcatc gcgacgaccg gaagatggtc cgaggcgctc acgcgcggca cctcacacga
     5221 caccacgtca atgccgcggg cgaagacgta gtcgatgcgc cggtcgggac cgttggaggg
     5281 aaacgtcgcg cccccgcctt ccccggccac ggcgaaggcg tcggtgaagt gccccgcgag
     5341 cctgcggagc gctgccccgt ccgcgggctc gttgaagtcg cccgccagga tcgccggacg
     5401 cgcctgtccc acatggccga ggatctcctc gagatgccgc gcgcgctctt cgccggcgtg
     5461 gccgagatgg gtcgccgcga cggtgacgcc gccgcagtcg gcgatcagca gcccgcgtcg
     5521 ctccatcccg ggcgacttcg agaggcgtat gcgcgtgacg cggccgaacg gctcgcgcgc
     5581 caggatcgcg ttgccgaatc gccgcacccc gatcgtcggg ccgaagacgg cgcgacgccc
     5641 ggcgatacgg gcgatgggcc gcaggttcgc gccgcgaacc tcgttgaggc aagcgatgtc
     5701 gggggccagg tcgcgcagga cgccggcgac ggcctcgacg ccgcgcacgc ctccctgcac
     5761 gttgtaggtg acgacgcgga gcgtcacgca accacctcgg tgccggcggt gccctcgatc
     5821 gcgtcggcga cggattcgag cgaggtgatg atcgcgcggc gtccctcctc cgcggcctgc
     5881 acgaccgcct cgaccttggg tcccatcgag ccggctggga attcgcctgc ctcgagccgc
     5941 tcgcgggcct cggcgacggt catccggccg acggaccgct cgccgcgcgt gccgcggtcg
     6001 acgatcacgt gctcgacgtc ggtcaggatc accatcatcg ccgcctcggt cgcgcgcgcc
     6061 aggatcgcgg ccgcgagatc cttgtccacc acaccgtcca cgccgcgata gccgccgtcc
     6121 tcctcgatca ccggaacgcc ccctcccccg gccgcgatcg gcgtgatgcc cgcatcggca
     6181 agcgcgcgga tcgcggcctc ctcgaccagg cgcagcgggc gcggcgaggc gacgacgcgc
     6241 cgccagccac cgcgcatctc gtcgcgggcc acggcgatgc cgcgcgcctc gagcgcgcgc
     6301 gcggccggcg cgagcatcga cgggccgacc ggcttggtgg gatcggcgaa ggccggatcg
     6361 ccggccgcga cgacagtctg ggtgatgacg gtcgcgaccg gccgcggcga gagcgtggcc
     6421 gaaagcgcct gctgcagcat gtagccgatc tcggcctggg actcggcgcc gagcacgtcc
     6481 agcggcatca tcgggatgcg ccgctgcgcc gcgaggttct gcaacagcag ccggccgacc
     6541 tgcgggccgt tgccgtgcgt caccaccagc tcgatgccgc gggaggcgac gacgcccagc
     6601 ccgctcgcgg cctgggcgat cgcggcgcgc tgctgcgcga ccgtgccacg ctggccgtgt
     6661 cgaacgatcg cgttcccgcc cagcgcgacg acggcccgca tcgtcctacc gggtgagcgg
     6721 cgccagcacg tcggcgagcg tcggcgagcc gtgctcttcg aggtcgtagg tcacgcgcac
     6781 ggccggcttg gagaactcgg cgacgcgacg cagatccacc ggcgtcgcga tcacgaccac
     6841 gtcggccggc gtcgcgtcga tcgtggcggc gaggtcgctc atctgctgct tgccgtagcc
     6901 catcgcgggg agaagcgtgc cgatctcggg gtaggacgcg aacgtctccc gaatcgagcc
     6961 gaccgcgtgc tcgcgcggat cgacgatctc ggccgcgccg gcgttgcgtg ccgcgaccac
     7021 accggccccg tacttcatct cgccgtgcgt gagcgtgggg ccgtcctcga cgaccagcac
     7081 ccgcttgccc ttgatcgcat ccggatggtc cacggtgacc ggcgagtggg ctctcaccac
     7141 ggtcgcgccg gggttgagct cgcggatcga cgcctcgatc tcggcgacct gctcggccgt
     7201 ggcgctgtcg atcttgttga tcaccaggac gttggcagcg cgcacgttgg cctcacccgg
     7261 gtggtagcga ttctcgtgcc cggcgcgcaa cggatcgacg acggtgatgt gcacgtcggg
     7321 ggtgtaga
//
LOCUS       DATLFO010000102         3447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_2213, whole genome shotgun sequence.
ACCESSION   DATLFO010000102 DATLFO010000000
VERSION     DATLFO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..492)
                     /locus_tag="VM841_03490"
     CDS             complement(226..492)
                     /locus_tag="VM841_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89281.1"
                     /translation="MGQSAAETVAEIEQIRDRLDGEIHELQERMPAAVVAGKRIVGIA
                     VSGGVAGSLFWMLVRRRRKRKRAKSGVSLGDGKTFTAEIRLIPS"
     gene            complement(465..1793)
                     /locus_tag="VM841_03495"
     CDS             complement(465..1793)
                     /locus_tag="VM841_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HVL89282.1"
                     /translation="MSIVTDAMRRIDSVQRRHAALAFPVAVFKKFGEDRGGRLASLIA
                     YYGFFSLFPLLLVFVAVLGFLLGDNPQLQRDILDSTLANFPIIGDQIRSNIGSLSGNG
                     FALAIGLAGALWAGMGAIRATQTAMDDLWSVPRERRPNLIGSAVRSLVMLTVLGTFAV
                     LAAIIPTLGSAFGTGFGLRVAGIALTLLVNFGLFMVVFKVLTVAKVSWRDVTPGAALA
                     AVVWSILHSIGGWYLGSRLEGASQVYGFFAIVIGLLAWIALAAQVTLLAAETNVVRIR
                     GLWPRALSEERVGGQKEGPDASVRPFVKPDRSGAMETANNNRTSTPQLVRSIADDTRT
                     LVQKEIELARHEIVEAVLARIKGGVAFIGAGIMMLFVLVFAGSSIAHALEGRMSAWAA
                     RLIVAAGFLVLAVGAALFGKMRMSKPAIAPEETKRTVKEDIEWAKAQLKR"
     gene            complement(1934..2305)
                     /locus_tag="VM841_03500"
     CDS             complement(1934..2305)
                     /locus_tag="VM841_03500"
                     /inference="COORDINATES: protein motif:HMM:NF018867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HVL89283.1"
                     /translation="MASIDGVVFEGIDTVPYAAVRLLAPGGGVVAEQTANSAGEFRFV
                     VPSGLWGIEANAGDQKIAVRDVMAFDGERFPVYVCLDSGERRTFRRSRRAGERATKMR
                     PAQSRPARVVEMPGVSAGDRS"
     gene            complement(2441..2710)
                     /locus_tag="VM841_03505"
     CDS             complement(2441..2710)
                     /locus_tag="VM841_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89284.1"
                     /translation="MSPAKKKAASAKKRTAPSPKKAAKKKAAEKPRETKSCRMCGTTA
                     TCAPGGIPPGWSFSAEPGGITFMCDDCARRNIRSIEAKLSMEWWE"
     gene            2809..>3447
                     /locus_tag="VM841_03510"
     CDS             2809..>3447
                     /locus_tag="VM841_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89285.1"
                     /translation="MGRRARIAAAAALMIAATLPATPVAAGPHPAAPACEIFPANNVW
                     HADVSSLPVHARSADWLASMGGSGRRLHPDFGPSGLSQPYGIPYNVVAGSHAKVSVNF
                     SYPDESDPGPYPLGDDTTIEMGSDRHALMLDRDACVLYETFDTWKDAGGWNAGSGAVW
                     DLRSNDLRPAGWTSADAAGLPIFPGLIRRDEIAAGVIDHAIRMTASPTDRSYV"
BASE COUNT          601 a         1179 c         1110 g          557 t
ORIGIN      
        1 ccctcgatcg gccggccgcc tcccgccccc ggcgagcaga ccgacgaggt gctcgccggc
       61 ggcggcttcg atcccgcaga gatcgcacga ctcaaggctg cgggcgcgct cggctgagcg
      121 tcccacatcg gggacggccg ccaccggcgg atgcgtcacg gtcctgccga aatgaagcat
      181 ccggcgccat cagggacgcc ggatgcgggt gtgccgaatc agcgatcagg acgggatgag
      241 ccggatctcg gcggtgaagg tcttgccatc gccgaggctc acgccgctct tcgcgcgctt
      301 cctcttgcgg cggcgccgaa ccagcatcca gaatagggaa ccggcgaccc cgccgctgac
      361 cgcgattccg acgatgcgct tgccggcgac gaccgcggcg ggcatccgct cttgcagctc
      421 gtggatctct ccgtcgagcc ggtcccggat ctgctcgatc tcggctaccg tttcagctgc
      481 gctttggccc attcgatgtc ctccttcacg gttcgcttcg tctcttccgg cgcgatggcg
      541 ggtttgctca tccgcatctt tccgaacagg gcggccccga ccgccagaac caagaacccg
      601 gctgcgacga tcaggcgcgc ggcccatgca ctcatgcgtc cttcgagggc gtgcgcgatc
      661 gacgatccgg cgaagacgag cacgaagagc atcatgattc cggccccgat gaacgccaca
      721 ccacctttga tgcgggcaag caccgcttcg acgatctcgt gccgcgcgag ctcgatctct
      781 ttctgaacga gcgtgcgcgt atcgtcggcg atggaccgca cgagctgcgg ggtcgaggtt
      841 cggttgttgt tggccgtctc catggctccg ctcctatccg gcttgacgaa cggacgcacc
      901 gaggcatccg gtccttcctt ctgtcctccc actcgctctt cgctcagcgc ccgaggccac
      961 aggccccgga tgcgcacgac gttcgtctcg gcagcgagca gcgtcacctg cgcggcgagc
     1021 gcgatccagg ccaaaagacc gatcacgatc gcgaagaatc catagacctg cgacgcgcct
     1081 tccaaacggc tgccgagata ccagccgccg atcgaatgca ggatcgacca cacgacggcg
     1141 gcaagagcgg cgcccggcgt gacgtcgcgc cacgaaactt tggcgacggt cagaaccttg
     1201 aacaccacca tgaacaaccc gaagttcacg agcagggtca gcgcgattcc ggccacccgg
     1261 aggccgaacc cggttccgaa cgcgcttccg agcgtgggaa tgatggccgc gagaacggcg
     1321 aaggttccca gcaccgtcag catcaccagc gagcgcacgg ccgagccgat gaggttcggt
     1381 cgccgttccc gcggcacgga ccacagatcg tccatcgccg tctgcgtcgc gcggatcgcg
     1441 cccatgcccg cccacagcgc acccgcaaga ccgatagcca atgcgaaacc gttgccggaa
     1501 agcgagccga tattgcttcg tatctgatcg ccgatgatcg ggaagttggc aagcgtggaa
     1561 tccaagatgt cccgctgcag ctgggggttg tcgccgagca agaacccgag aaccgccacg
     1621 aaaaccagca acaagggaaa cagcgaaaag aagccgtagt aggcgatgag cgaggccagg
     1681 cggccgccgc ggtcctcacc gaactttttg aagaccgcga ccgggaaggc aagcgcggcg
     1741 tgccgtcgct gcaccgaatc gatccgccgc atcgcgtcgg tgacgatgct caccttcctt
     1801 ttgtacccgg tggtcgcctc gctaaccagg ggcgaatagg cttgcttgtc tcgaacgcaa
     1861 ccgaaagagc ctcaatgcac gttcatcctg ctcgcgagca atgcaccggc ttctccgaac
     1921 tgccgctgcg cggctaggag cggtcgccgg ccgacacgcc cggcatctcg acgacacgcg
     1981 cgggacggct ctgcgcggga cgcatcttcg tggcgcgctc accggcgcga cgagagcggc
     2041 ggaaagtgcg gcgctcgccc gagtcgaggc acacgtagac ggggaagcgt tcgccgtcga
     2101 aggccatcac gtcacgcacg gcgatctttt ggtcgcccgc gttcgcttcg atgccccaca
     2161 ggccgctggg gaccacgaaa cggaactcgc cggccgagtt ggcggtctgt tcggcgacga
     2221 cgccgccgcc gggggcgagc aggcgaacgg cggcataggg gaccgtgtcg atgccttcga
     2281 aaacgacgcc gtcgatggag gccacgatcg aacctttccc cggatagcct gaaagtatcg
     2341 ctccgttcgg ttacccatca tggaccgata tgtaacattt gtcaaggaag gatcgatccg
     2401 gcagggaatt cgggacaacg gatcaactgg ggagaacctg ctactcccac cactccatcg
     2461 acagcttggc ttcgatcgag cggatgttgc ggcgggcgca gtcgtcgcac atgaaggtga
     2521 tgccgccggg ctccgccgaa aacgaccagc cgggcgggat gccgccgggc gcgcaggtcg
     2581 cggtcgtgcc gcacatccgg cacgacttgg tttcgcgcgg cttttcggcg gctttcttct
     2641 tggcggcctt cttcggggag ggcgccgtcc gcttcttcgc cgaggcggcc ttctttttgg
     2701 cggggctcat cagccgatag cctaggacag cacccgccgg cgtcacggga ggcgaggcat
     2761 ttgcgggctt tctcaagcga ttcgcacgga ccggccgata catctcgcat ggggagaagg
     2821 gctcgcatcg cggcggccgc ggcgttgatg atcgcggcga cgctgccggc cacgccggtt
     2881 gccgccggcc cgcaccccgc ggcccctgca tgcgagatct tcccggcgaa caacgtctgg
     2941 cacgccgacg tgtcgtcgct gcccgttcac gcgcgctccg ccgactggct ggcctcgatg
     3001 ggcggcagcg gccgccggct gcatcccgac ttcggtccca gcggcctgtc ccagccctac
     3061 ggcatcccgt acaacgtcgt cgccggatcg cacgcgaagg tcagcgtcaa cttctcctat
     3121 cccgacgagt ccgatcccgg accgtatccg ttgggcgacg acaccacgat cgagatgggc
     3181 agcgaccggc atgctctgat gttggatcgc gacgcgtgcg tgctctacga aaccttcgat
     3241 acgtggaagg acgccggcgg atggaacgcg ggcagcgggg cggtctggga tcttcgctcc
     3301 aacgatctgc ggcccgccgg gtggaccagc gccgacgcag ccggcttgcc gatcttcccg
     3361 ggcctgattc gccgtgacga gatcgccgcg ggcgtgatcg atcatgcgat tcgcatgacc
     3421 gcatcgccga ccgaccgctc gtacgtg
//
LOCUS       DATLFO010000103         6544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_82254, whole genome shotgun sequence.
ACCESSION   DATLFO010000103 DATLFO010000000
VERSION     DATLFO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82254"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1155)
                     /locus_tag="VM841_03515"
     CDS             complement(226..1155)
                     /locus_tag="VM841_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVL89286.1"
                     /translation="MKTRTLGRSDLELSVIGYGAWEASEHWGPLEEDRLIEAMVAAFD
                     AGINWIDTAEVYGPYTSEELCGRALAERPAMMIATKVAPAPSGSGFAADQVRRACEDS
                     LKRLGRDTIDLYQLHWPDKNVPVEETWHAIAELADEGLARAIGVSNFPPELIEACEKI
                     RHVDSLQPHVSMLHRTNLDLVRWCGEREIGTVAYSPLACGLLTGTITSEWQFDPDDWR
                     AGDRGFALYEGLFGPQSLPGHLETVERLKPVAERLGVTLAQLAIAWVLDQDGITSAIV
                     GSRSAQHVASNVAAAGLALDDATRGEIRAALEG"
     gene            1214..1450
                     /locus_tag="VM841_03520"
     CDS             1214..1450
                     /locus_tag="VM841_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89287.1"
                     /translation="MTKPKVKTAMLGQFTHERAEAIAKELEEAGITWYYKQAGSIVQT
                     FFRGEWGVRLFADVSRADDVREIVKRVEERLGPA"
     gene            1457..1541
                     /locus_tag="VM841_03525"
     tRNA            1457..1541
                     /locus_tag="VM841_03525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1492..1494,aa:Leu,seq:taa)
     gene            1881..2819
                     /locus_tag="VM841_03530"
     CDS             1881..2819
                     /locus_tag="VM841_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HVL89288.1"
                     /translation="MAYDTNVQQRSADQAADRLAARQFGVFRLDQMLAEGVSKGRIRQ
                     RIVTGRWEPVARGVYRLRSVGPTWEQTLMISLLGWGAGTVASHRSAAALWSLVGFRRR
                     PIELTVPRNRRRTHPPPGTVYRGALTRADVTTTGAIPVTTPGRTLVDLAATCPRDPVE
                     EALDDALRRTLVTPGRMRAVLGRMCGRPGVTVMRELLEARTGMAPLESRHETRLLRVL
                     LKAGLPRPVPQYETFDASARLIARVDFAYPEYKIAIQADGFAFHGGRLQFQKDREQDR
                     TLQAMGWRVIRVTWEDAEHRPDRIAALIGAMLAAGR"
     gene            complement(2872..3450)
                     /locus_tag="VM841_03535"
     CDS             complement(2872..3450)
                     /locus_tag="VM841_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVL89289.1"
                     /translation="MSMITAARTMDGVEIPAAGTWDIDPTHTTVEFIARHLLVSKVRG
                     RFGSFSGSVIVADQPYQSSVSVSIDPASLDTGNADRDGHLRSSDFFDVDTYGEMTFVS
                     TGFTPTGKTTFDLPGTLTIRGIAKPVVLQGEFIGLATDPWGGTRAAFSAATEVDREDW
                     GLTWNKVVESGGVLVSKTVRIELEVQLIRKDT"
     gene            complement(3691..4188)
                     /locus_tag="VM841_03540"
     CDS             complement(3691..4188)
                     /locus_tag="VM841_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVL89290.1"
                     /translation="MSKRDGPLRAPGNLEQRYPTADKAATEVAINLLRADSLLAGALN
                     RRFRAYGLSIATFNVLMILEGASDALCPAEIGKRLLVTRGTVTGLLDSLEKQGLISRT
                     PDKQDRRMLRIQITPKALDLLDRLLPEHFKGEAELLAGLTPREKETLIKLLGKAQASV
                     SSSAG"
     gene            4248..5150
                     /gene="gnd"
                     /locus_tag="VM841_03545"
     CDS             4248..5150
                     /gene="gnd"
                     /locus_tag="VM841_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HVL89291.1"
                     /translation="MAGADERTTMQLGMVGLGRMGGNMAERLRDGGHDVIGYDAFADT
                     TQVATLEELVNKLDESRTIWLMVPSGEPTNQTIAALADLCRPGDVIVDGGNSYFRDSI
                     THAAMLAERGIVFVDAGVSGGVWGRDNGYCLMVGGPAEAVERIKPALETLAPPGGLVH
                     TGPAGSGHFVKMIHNGIEYGMMQAIGEGFEIMSASPDYPDIDLHAVAEAWRYGSVVRS
                     WLLDLAAAAMQSDPRLENIRGFVQDSGEGRWTILEAINQTVPAPVISAALFARFASRQ
                     DDSFAMKIIAALRREFGGHAVKEL"
     gene            5147..>6544
                     /gene="zwf"
                     /locus_tag="VM841_03550"
     CDS             5147..>6544
                     /gene="zwf"
                     /locus_tag="VM841_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVL89292.1"
                     /translation="MTAPKPQIAVIFGASGDLTHRKILPAFYNLLRHGSLPEQFAILG
                     YARSGWDDARFRERARQSLEEFTPSTFDQETWKRFEDMIFYQSGPFDEADCFTPLWER
                     LDALDRERGTESRRLYYAATPPSAFPLIVERVGECAPRPDTRLVIEKPFGRDLESARR
                     LNDQMHEVFDERQIFRIDHYLGKETVQNILVFRFGNALFERVWNRDAIDHVQITVAEK
                     GGIEGRGAYYTEAGALRDIVQNHVLQVLAFLTMEPPRSFSADAVRDETLKVLRSMRPM
                     DPAAVVRGQYEGFLDEEGVPKDSLTETYFAARAEIDNWRWSGVPFFLRTGKRLPRRVT
                     EITIRLRSVPVSLFESAGVEHLTSDHLTIRIQPNEGISLAFNAKRPGPGFEPEPVRMD
                     FDYDETFGADPADAYERLLLDALEGDGTLFTREDGVERAWEVVEPLLADRGSVCSYAS
                     GSWGPKEADDLIAPRT"
BASE COUNT         1068 a         2240 c         2159 g         1077 t
ORIGIN      
        1 gcgctccgcg cgcgccgatc aacggggaac gtcaagcccg agccctgcgc tccgcgcgcg
       61 ccgatcaacg gggaacgtca agcccgagcc ctgcgctccg cgcgcgccga tcaacgggga
      121 acgtcaagcc cgagccctgc gctccgcgcg cgccgatcaa cggcgaagcc gcaatccggc
      181 agactccggc atttcgattc caccggacat tattgccagt ggaaactatc cttccagcgc
      241 cgcccggatc tcaccgcgtg tcgcgtcgtc cagcgcgagc ccggccgcgg ccacgttcga
      301 ggccacgtgc tgcgcgcttc gcgagccgac gatcgccgac gtgatgccgt cctgatccag
      361 cacccacgcg atcgcgagct gcgccagggt gacgccgagc cgctcggcga ccggcttgag
      421 ccgctcgacg gtctccagat gccccggcag actctgcggc ccgaacagcc cttcgtagag
      481 cgcgaagccg cggtcgccgg cgcgccagtc gtcggggtcg aactgccact cggacgtgat
      541 cgtgccggtc agcagcccgc acgccagcgg ggagtacgcc acggttccga tctcgcgctc
      601 gccgcaccag cgaaccagat ccaggttcgt gcggtgcagc atcgacacgt gcggctgcag
      661 cgaatcaacg tgacggatct tctcgcacgc ctcgatcagc tcgggcggaa agttcgacac
      721 gccgatcgcg cgggcgaggc cttcgtcggc tagttcggcg atcgcgtgcc aggtctcttc
      781 gacgggcacg ttcttgtcgg gccagtgaag ctggtacaga tcgatcgtgt cgcggccgag
      841 ccgcttgagc gagtcctcgc acgcacggcg aacctggtcg gccgcgaagc ccgagcccga
      901 cggggcgggc gcgaccttcg tcgcgatcat catcgccggc cgctcggcga gcgcgcggcc
      961 gcacagctcc tcgctggtgt acggcccgta gacctcggcc gtgtctatcc agttgatgcc
     1021 cgcgtcgaaa gccgccacca tcgcctcgat gagccggtct tcctccagtg gcccccagtg
     1081 ctccgacgcc tcccacgcgc cgtagccgat caccgacaac tccagatcac ttcgtccaag
     1141 cgttcgtgtc ttcatacgtc gcatccttgc ggtcgtgccg tccgccgtca aagcggggta
     1201 ccctgatacc gcgatgacca agccgaaggt caagaccgcg atgctcgggc agttcaccca
     1261 cgagcgagcc gaggccatcg ccaaagagct cgaggaagcc gggatcacct ggtactacaa
     1321 gcaggccggc tcgatcgtgc agaccttctt tcgcggcgag tggggcgtgc gcctgttcgc
     1381 ggacgtctcc cgcgccgacg acgtccgcga gatcgtcaag cgcgtcgaag aacgattagg
     1441 cccggcgtag caacgcgccc gggtggcgaa actggcgaga cgcatcggac ttaaactccg
     1501 aggcccgcaa gggcatgagg gttcgaatcc ctccccgggc acgatccgga ttctggccgg
     1561 gaaataaaaa agggttccga gtgacttcgg gtccgtcttt cgacttatcc ctctaccgct
     1621 cggaaccgac ttcacggtac cgcccgggcc gcgccgcccg caaggggagc gcggccccca
     1681 aaaggcagcg cggccgatag tccgttcgag cccgcccgcg ggctacgggg tgcgcgcgac
     1741 gtagatcagg ttgaacggat cctcttcgag cggcgcggtc tgcacgtcgc cgaagcaaca
     1801 ttattgcgcc gaacggcgcg atcaccccac caacccgagg ccattcgccc cgcgcgtggt
     1861 agtcgttgtt gcgatccggc atggcatacg acaccaacgt gcaacaacga tcggccgatc
     1921 aggccgccga ccgtctggcc gcgcgccagt tcggcgtctt ccgcctggat cagatgctgg
     1981 ccgagggagt ctcgaaaggc cggatccggc aacgcatcgt caccggccgg tgggagccgg
     2041 tcgcgcgcgg tgtctaccgt cttcgcagcg ttgggcccac gtgggagcag accctcatga
     2101 tctcgcttct cggatgggga gcagggaccg tggcctcgca ccgttcggcc gcggcgcttt
     2161 ggtcgctggt cgggttccgg cggcggccga tcgagctgac ggttccgcgc aatcgcaggc
     2221 ggacgcatcc gccgcccggc acggtgtacc gcggtgcgct gacgcgagcc gacgttacca
     2281 ccaccggcgc gattcctgtc accacccccg gccgcacctt ggtcgatctg gcagcgacct
     2341 gtcctcgcga tccggtcgag gaagccctcg acgatgcgct gcgccgcacc cttgtcacgc
     2401 cggggcggat gcgggcggtg ctcggccgga tgtgtggtcg tcccggcgtc acggtgatgc
     2461 gcgagctgct cgaggcccgc accggcatgg ccccgctcga gagccgccac gaaactcgcc
     2521 tgctccgcgt gctgctgaag gccgggctgc cccggccagt gccgcaatac gagaccttcg
     2581 acgcgtccgc ccgtctgatc gcccgcgtcg atttcgccta tcccgaatac aagatcgcga
     2641 tccaggccga cggcttcgct ttccacggcg gccggctcca attccagaag gaccgcgaac
     2701 aggaccgcac gctgcaggcg atgggctggc gcgtgatccg cgtcacctgg gaggacgcag
     2761 agcatcgccc cgatcggatc gctgcgttga tcggggcgat gcttgcggcg ggtcgttagc
     2821 gtccttgggg tcgttcggtg tccttggggt cgttaggtgt ccttggggtc gttaggtgtc
     2881 cttacggatc agctgcactt ctagctcgat gcggaccgtc ttgctcacca gcacgccgcc
     2941 ggactcgacg accttgttcc aagtcaggcc ccagtcttcg cggtccacct cggtcgcggc
     3001 cgagaacgcc gcgcgcgtgc cgccccacgg gtcggtggcc aggccgatga actcgccctg
     3061 cagcacgacc ggcttggcga tgccgcgaat cgtcagcgtg ccgggcagat cgaacgtggt
     3121 cttgcccgtc ggcgtgaagc cggtgctgac gaaggtcatc tcgccgtagg tgtccacgtc
     3181 gaagaagtcg gaggagcgga gatgcccgtc gcgatccgcg tttccggtgt cgaggctggc
     3241 cgggtcgatc gagaccgaga ccgacgactg atacggctgg tccgcgacga tcaccgagcc
     3301 cgagaacgag ccgaagcggc cgcgaacctt gctgaccagc aaatgccggg cgatgaactc
     3361 gacggtggtg tgtgtgggat cgatgtccca ggtgccggcc gccggaatct cgacgccgtc
     3421 catcgtcctg gctgcggtga tcatgctcat ggtgtttcct cccgaaggct ccgggggcga
     3481 tcatcgtatt tgaccgccct gctataagcc tccaagtagt ttagagccaa actatattcc
     3541 cggatcccga acgtttccga tccttgaaga tcggtggccc atttgggatt ggaggcgcgg
     3601 cgcaggtggt gcggccgctg gtgcggcttc gttaggacgg ccgctggtgc ggcttcgtta
     3661 gggcggccgc tggtgcggcc tcgtcaggcc ctagccggcc gaggacgaga ccgaggcctg
     3721 ggctttgccc aagagcttga tcagggtttc cttttcgcgg ggggtgaggc cggccagcag
     3781 ctcggcctcg cccttgaaat gctcgggcag aagccggtcc agcagatcca gggccttcgg
     3841 cgtgatctga atccgcagca tccggcggtc ttgcttgtcc ggcgtgcggc tgatcaggcc
     3901 ctgcttttcg agcgagtcca gcaggccggt gacggtgccg cgcgtgacca gcagacgttt
     3961 gccgatctcg gcggggcaca gcgcatcgct ggctccttcc aggatcatca agacgttgaa
     4021 ggtcgcgatg gacaggccgt aggcgcggaa gcgacggttc agcgccccgg ccagcagcga
     4081 atccgcgcgc agcagattga tcgcgacctc ggtcgcggct ttgtcggccg tgggatagcg
     4141 ctgttcgagg ttgccggggg cccgcagcgg cccgtcgcgc ttgctcatgt cgatcgctcc
     4201 cagatagtac gcatccaaac tagtctagca tgaggaacaa tgagatgatg gccggagccg
     4261 acgaaaggac gacgatgcag ctgggaatgg tcggactggg acggatgggc ggcaacatgg
     4321 ccgagcgcct gcgcgacggc ggccacgatg tcatcggcta cgacgccttc gccgatacga
     4381 cgcaggtggc caccctggag gaactcgtca acaaacttga cgagtctcgc acgatctggc
     4441 tgatggtccc gtccggcgag cccaccaacc aaaccatcgc ggccctggcc gacctctgcc
     4501 ggcccggcga cgtgatcgtg gacggcggca actcgtactt ccgcgactcg atcacacacg
     4561 cggccatgct cgccgaacgc ggcatcgtct tcgtggacgc gggcgtgtcc ggcggcgtgt
     4621 ggggccgcga caacggctac tgcctgatgg tcggcggccc cgccgaagcg gtcgagcgca
     4681 tcaagccggc gctcgagacg ctcgccccgc ccggcggcct ggttcatacc ggccccgccg
     4741 gcagcggcca cttcgtcaag atgatccaca acggcatcga gtacggcatg atgcaggcga
     4801 tcggcgaagg cttcgagatc atgagcgcct cgcccgacta ccccgacatc gacctgcacg
     4861 cggtggccga agcgtggcgc tacggctcgg tcgtgcggtc ctggctgctg gatctggccg
     4921 cggccgccat gcaaagcgat ccgcgtcttg aaaacatccg cggcttcgtg caggactcgg
     4981 gcgaaggccg ctggacgatt ctcgaggcga tcaaccaaac cgtgcccgcg cccgtgatca
     5041 gcgcggcgct gttcgcgcgg ttcgcctcgc ggcaggacga ttcgttcgcg atgaagatca
     5101 tcgccgcgct tcggcgcgag ttcggcggtc acgcggtgaa agagctatga ccgcgcccaa
     5161 gccgcagatc gccgtcatct tcggcgcgtc cggcgatctg acgcatcgca agatcttgcc
     5221 ggccttctac aacctgctcc gccacggctc gctgcccgag cagttcgcga tcctgggcta
     5281 cgcgcggtcc ggctgggacg atgcccggtt ccgcgaacgc gcacggcaaa gcctggaaga
     5341 gttcacgccg tccacgttcg accaggaaac ctggaagcgc ttcgaagaca tgatcttcta
     5401 ccagtccggg ccgttcgacg aggccgactg cttcacgccg ctgtgggagc ggctggacgc
     5461 gctggaccgc gaacgcggca ccgaaagccg ccgcctctac tacgccgcca cgccccccag
     5521 cgcgttcccg ctgatcgtgg aacgcgtcgg cgaatgcgcg ccgcgtccgg acacgcgcct
     5581 ggtgatcgaa aagcccttcg gccgcgacct tgaatccgcg cggcgcctga acgatcagat
     5641 gcacgaggtc ttcgacgaac gccagatctt ccgcatcgac cactatctcg gcaaagagac
     5701 cgtgcagaac atcttggtgt tccgcttcgg caatgcgctg ttcgagcgcg tctggaaccg
     5761 ggacgcgatc gaccacgtgc agatcaccgt cgccgagaag ggcggcatcg aaggccgcgg
     5821 cgcctactac accgaggccg gcgcgctgcg cgacatcgtg cagaaccacg tcctgcaggt
     5881 gctcgcgttc ctgacgatgg agccgccgcg ctcgttcagc gccgacgcgg tccgcgacga
     5941 aacactcaag gtccttcgct cgatgcggcc gatggatccc gccgccgtcg tgcgcggcca
     6001 gtacgaaggc ttcctggacg aagagggcgt gccgaaggac tctctgaccg aaacgtattt
     6061 cgccgcacgc gccgaaatcg acaactggcg ctggagcggc gtgccgttct tccttcgcac
     6121 cggcaaacgg cttccgcgtc gcgtgaccga gatcacgatc cggctccgct cggttccggt
     6181 ctcgcttttc gagtccgcgg gcgtcgagca cttgacctcc gaccatctga cgatccgcat
     6241 tcagccgaac gaagggatct cgctggcctt caacgccaag cggcccggtc ccgggttcga
     6301 gcccgagccg gtccggatgg acttcgacta cgacgagacc ttcggggcgg acccggcgga
     6361 cgcgtacgag cgtttgctgc tcgacgcgct cgaaggcgac ggcaccttgt tcacgcgcga
     6421 ggacggcgtc gagcgagcct gggaggtcgt cgaaccgctc ctggccgatc gcggctcggt
     6481 ctgctcctat gcgagcgggt catggggccc gaaggaggcc gacgatctga tcgcccctcg
     6541 cacc
//
LOCUS       DATLFO010000104        46413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_44113, whole genome shotgun sequence.
ACCESSION   DATLFO010000104 DATLFO010000000
VERSION     DATLFO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 46413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46413
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44113"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VM841_03555"
     CDS             <1..553
                     /locus_tag="VM841_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89293.1"
                     /translation="FLAIPYMVWRALGRRDQNAALLLILFAPLYLPWLVTTRPLFLFY
                     MTPAVPILALMVAHMVDRITEEMRAWQLTVMFALMFTLPFVPLYIQWLHAGRTSHLLY
                     KIPALAVIFLVANGIATLLAYTPKPRRLILVYGGLVLAMFAYFYPVLAAGHIPDGGTF
                     GWRKHMWLQRDCGQQGILLTCWI"
     gene            777..3125
                     /locus_tag="VM841_03560"
     CDS             777..3125
                     /locus_tag="VM841_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HVL89294.1"
                     /translation="MTSHRAFRAAVLLLMASFFAMPLKAQADPCAAVLCAGAATADIT
                     PPVTTPQWGYTNRQGLLFSASGVQSHITGGNPAGVAQERFVRDKTNGEIELYNKEFVA
                     SRGIHLRLQANAFVIQGANGQRVAVVQADLGGIPGELHKAVAKRIAAVTGIAESHLLI
                     SATHTHGGPGGFFQYQGYALLGGDEYDPRVFDAVATGLTNAVVTAYNMLAPAKMASTR
                     IAAPGANRNRRVAQWCRNPEALCNGTTPTPASPPAHDDDTTVFRIDTATGLPLGVLTQ
                     FPAHGTVGGDGNLLFSGDNQGWATRKVVEGIRAASAPLPDGHVIVNALVNGAQGDVSA
                     VGSGFNQYASMEDAGMRQAAPVLSAWAALKPALRTDVTVDARFEHVCFCGQRIDDEFL
                     YDGPAIDKRDPMWDRVAPYASLGATIVNGPRTTPVPTPAQGNKTPGLVGAGLVPSIVR
                     LQTLRVGDVLFASIPGEANVQVGRRIVNRLMSLPGRPWSTVVIGGLGNDYISYFVTPE
                     EYTAQEYEGGFTLFGPQSSPLMEQELTRLARAMIAGVPVAPCDPSVDAGDCLEHLAHP
                     DTTAAAVTPVAPDLGGSAAVVAAATGTARLSHASMVWHGGGPTAEWRPGADRVAIERL
                     DGSEWVDVAGDAAGGATTIRYEKIDGKHTWMTVWDVPADAAAGRYRFRVEGRTATSPF
                     TFSPYLLTSEFDVLGSNAMAVTVTKGSSWTVRATYPVDPAVSFRIRPLVAGTATLVVV
                     RGGVETTLTAAMSGGVANFALEAGDTLVGASVVDAHGNTGAA"
     gene            3122..5248
                     /locus_tag="VM841_03565"
     CDS             3122..5248
                     /locus_tag="VM841_03565"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HVL89295.1"
                     /translation="MRRRLLAALLALAAIAPANASRAATVVADEDAFVRLNGSFLVIG
                     NDLIERFWTLGGETVSVTTHKGAFTGGWSSDTQFGTGAPDLWRIRAWSASRRPAGGVE
                     LRMRMAGARPYEIERIARVLPGVSAVRMETIVHAGPAPVVLPGYTLESLSNPLSAPAA
                     RAINFSGGADWRPAYDYRRDTVGPQFDANAEWLETADGGLGLLMQRRNYLSSRMQWSG
                     HRARATVDLSHDKAYLGPFEEIRPEGGHPPAAPARTRTVEPFRPLALEPVIVAMGTGP
                     DDLTWQAHRMLMSDVAPYPRTINFNTDRVRGAPFELGARDGVGLPQFNQLWPVAKAMG
                     VETFVLDDGWQLYNGDWLEPDAARWPNGLEEPRDILEANGMRLGLWMAPGSFHPHSNA
                     WKADPEATCLPVGAGTAAYNAASPNTGSNAAGIGVWDFNAPLAGELRRYRDKLASEIA
                     TLRATLRPTEFKFDFLVWLDCAFGTMYEQRDAFFDEIAALSGTFPGTGFGMDETNDYR
                     SFPYESILYGPTWFQNGNPSQDAVLHNVWTMAPYVPGFTIGQAATIRPGDPAAVIDEK
                     MAAALTYHMTFWTDIRELAGATAVTDRVRVWTDFAKQHRMTNGFAYPLLSDPLGRASW
                     SGMQPWDAETHKGMALLFRFDSPNATQTVSLRGLSASTTYRLTDVTPTEASFVVGEFS
                     GADLMDGIPVTIAQTRGVRVLTIDPA"
     gene            complement(5298..6374)
                     /locus_tag="VM841_03570"
     CDS             complement(5298..6374)
                     /locus_tag="VM841_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89296.1"
                     /translation="MTWTSDGYDMMLPVPLRAAPAPPPAAPTVAPGGAIPIVPPRGTR
                     SPKVIAVLFAVGGLVGGFIAYGAMTLIELILSFMGSSDSAVPEIAGITVALVGFGFAA
                     YFWLRADRETCVVTVGSQTMTISLPEWSQPMVVPREAVKLVALDDRPMIPFNKNHRFA
                     IEGELPEAVFADALDRHGRNPWEPVPAQVPYQLPQTTPPPDPDASNSDAPEPAEHEAG
                     WATVGSKPVPEPRGRHGYLYSGDGSALPLFLYNEIDIPNMAIVFTREMQLPKRSPGFF
                     LLQRRSIRSSNARKARGLMLRARNGVQAEAALAPWGVVRAPHAGDVLGAGLRPPKPLK
                     GLRAAAFAAMMIVPIVLDFVMRRA"
     gene            6452..7222
                     /locus_tag="VM841_03575"
     CDS             6452..7222
                     /locus_tag="VM841_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HVL89297.1"
                     /translation="MNLQGKTALVTGGASGLGRATVETLHAAGANAVIVDLPHAPGDE
                     LVKNLDERAVFSPADVTNTEEVQAGITAAVEHFGGLHIAVNCAGVGWAARTLSKDGPH
                     DLGVFSKVVEVNLIGTFNVIRLAAAQMASQEPAAGDEERGVIVNTASIAAFDGQIGQA
                     AYSASKGGVVGMTLPIARDLARMKIRVCTIAPGTFDTPMLAALPQEHRDALAAAIPNP
                     SRLGDPAEFGALVKHICENSFLNAETIRLDGALRMGPK"
     gene            7222..8544
                     /locus_tag="VM841_03580"
     CDS             7222..8544
                     /locus_tag="VM841_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HVL89298.1"
                     /translation="MPDIGALLPQIRADLEELVRIPSVSADGFDPAHVRASASKTLEL
                     FEAEGLSTELLEIDGAHPAVLATFPAPAGAPTVLLYAHHDVQPPGEESKWTSPPFEPT
                     ERDGRLYGRGTADDKAGIMVHLAAIRAFEGTPPVGLTILIEGEEETGSEHLGAFLSAY
                     GDRLRADAIVLADSGNWRIGEPALTTSLRGLVDCVVEVRTLDHAVHSGMYGGPIPDAL
                     TCLARLLSTLHDPDGNVAVGTRVHGSADPLDLTEDELRAQAGVADGVRMIGGGALTER
                     LWARPAISVLGIDAPTVQAASNQLVPVARAKISMRIAPGDDPSQALDELVAHLERNAP
                     WGAKVTVRRGSTGRPYALKADGPIYEAARQAMTEAWDGKKPVHMGVGGSIPLVAAFAD
                     RYPDAAILLTGVEDPDTRAHGENESLHLGEFARAIRAETLLLEHLGAR"
     gene            8809..9975
                     /locus_tag="VM841_03585"
     CDS             8809..9975
                     /locus_tag="VM841_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL89299.1"
                     /translation="MKRHLVIFFAIVAALGAVMPARAQSDRLEEARRKIRQYQDTSLV
                     GLLRAADDKRRAIEESIARIEREIVDVQKLIDLYKPELERAKIERDLASQAVIAATGA
                     YKNSRFMLGANALSMYATGQLPEMFVLMGTGDISDYVTARVYMDTVVDSSSRVVDGFR
                     RSRDRLEKEKARVETQTQAILDRGTKLEAERARLVKLAESKQKIAQDLDAASAAREEA
                     IAAIKSDPAGFDSILASYGSATEAIRMLVVAAQRGQPVQDPEKAAVWVPVDGRVSSKY
                     GWRIHPIFEKRSFHTGTDIAAPYGADVRAASPGTVVDVVYLGAYGLVVVVDHGFSLAT
                     MYAHLARSLVHPGERVQAGQVIGKVGCTGWCTGPHLHFEVWSRGNPQNPQAWVS"
     gene            10033..11013
                     /locus_tag="VM841_03590"
     CDS             10033..11013
                     /locus_tag="VM841_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVL89300.1"
                     /translation="MNMRAAISQRPHAARVALALGLVYIVWGSTYLAIRVTVETIPPL
                     LSAGVRFLTAGAILYAFAIRRTPKDERPTPIHWRSAAIVGTALLLGGNGGVVLAESWG
                     VPSGIAALLVGTVPLWMALISWVAFRDKLPAVGIAGLLAGFTGVAILAKPSGDFPLAG
                     SLLIVLAALIWSSGSVYAQHAPLPPRPFLTTAMEMLTGGASLLIVGLLRGEAAGVDLA
                     AFSGRSISALVYLIFVGSLVGYSAYIWALHNTRISLVSTYAYVNPVIAVILGAAILSE
                     PFSALDLIAGSIIVGSVALIVAAKKPTPPASPADAPPPPDEAIEAQPARN"
     gene            11046..11813
                     /locus_tag="VM841_03595"
     CDS             11046..11813
                     /locus_tag="VM841_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HVL89301.1"
                     /translation="MQAFGAASASVWAWSLLSVTGISAIALVGGLTFLLREQVLRPVV
                     LLLVSFAAGALLGDAFIHLIPELAEEAGFDLSLSLLILAGVGVFFVIEKVLHWHHAHL
                     PTEEVIHPVAWTNLIGDGIHNFIDGTLVAGAFLTDTKLGIGTAIAVALHEIPQELGDM
                     GILVHAGMSKKRALLLNLSTALVSLAGVALALLLAGAVDDLSRILLALTAGGFVYIAG
                     SDLIPELQRETRIPTAILQLTGLSLGVGLMAALTLLE"
     gene            11931..14084
                     /locus_tag="VM841_03600"
     CDS             11931..14084
                     /locus_tag="VM841_03600"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL89302.1"
                     /translation="MSRLYRTAAGVAALIAIGLAPITVPASKPPKHAAIRFAPNVVVD
                     PARMGGEPIMVTDSTGNIYISSIIGFSNHTSFLWKSEDGGESFDLLRVPLPAIQRPDF
                     TLGGGDTALIVGPPAPGYKDDTLIFIDLEGLASFGTATTFDGGNTFVNDNVFASGDQP
                     LGDRQWGGHWRDKNGVDHYYNFFNGLFSPDSGTSTASYAIIETTDYGKTWKDWKRNVV
                     TTPGRSRPGPLFVDPDTGDLMLTWTINRSGTGGAGFTLCNQLKRCEDTIIAEMPSYDT
                     NNTFVTGARDRQGNLYVAWSAIPRGELDPAEVPTRIYLSSSRNKGRTWTKPAVVSGNL
                     GAASMPSIVAGDSGRVSVVYYGTPKQGNPNDNAGPWHVYMSQSLNALDAKPSFTRAQV
                     TDHTNHINPICTMGLGCTLDPNRRDDRNLIDFLYAAIGKNGETLVTYGDTAHQIGPNP
                     PASSSITMFAKQIEGPSLYASVGELTPPEPDVLPYSGGQNRVSSYWASAVPDNWHADL
                     VKDVPFPRHGPGGPGPQNPSLDITSAWLEAAGPNTMRGTMMLRDTFSVKIPQPYTNNF
                     YMFWWWSKNKVHYAAAEVGPAEYGISQTEDAISDCYAGEPSYSNPASARWALYVAAAV
                     PPPNVTRIDCHLDLLTGRLTMDIPLDAVGAGIGDTLYSVTASSSAIHIPNIAFNAVAN
                     LPDEADAVSPFTYRVGSIRQQTTKLLRPPPRSKRR"
     gene            14250..15203
                     /locus_tag="VM841_03605"
     CDS             14250..15203
                     /locus_tag="VM841_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL89303.1"
                     /translation="MLALALSAFGLMPQTAHAASTKQKLNAMANRINRLQSALATSER
                     RAASMNKSIASASSRLKAARAGMTALTQGLYMSAFDETTALLAAESVAEFGERVAFAE
                     HLQSGQKDVLERVAIARTQLERQRKALAGVLAKQKELLKNLNKEQAALASEFRKEQQR
                     LAAIERAKRAKNRQRASRADAPSLISGTGPFRVCPVDEPRAYSNDWGAPRSGGRSHEG
                     TDILAPRGTPIRAPFDGTVKANSSGRGGKQVRVYGSEGYVFNAHLDRHAGVSGFVKAG
                     TVIGYVGNSGNASGGPTHNHFEWHPRNGGAVNPFRYLNKVC"
     gene            15278..16408
                     /locus_tag="VM841_03610"
     CDS             15278..16408
                     /locus_tag="VM841_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVL89304.1"
                     /translation="MELGLVILGLSVLARLALRIGIPPIPLYVIAGLAFGDGGLLPLV
                     TTQEFIRIGAEIGLILLLFMLGLEYPARKLIGTLKHAPRSGGLNIALNFTPGFVAGLL
                     MGWAPLPAAFLGGATYVSSSGVAARLIEDLRIGQEERTPIVSTLILEDLAMAMFLPTI
                     GAFLIGGSAAGSLMIAVSAVAIVILLLLFALRAGTGVSRLVFANADEPLLLSILGFGL
                     LIAGIAEVGQVSAAVGALVAGIVISGPAAQNVRPLLSPIRDLFAAIFFAFVGFSIDPG
                     QIPGALPVALLLAGITGATKFVAGWKAAEWAGVPWPRRSIVGATLLARGEFSLAIAGL
                     AVAARVRPNPAPIIATYVVIMALIGPVVARIVSIRVQRAEAD"
     gene            complement(16414..16629)
                     /locus_tag="VM841_03615"
     CDS             complement(16414..16629)
                     /locus_tag="VM841_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HVL89305.1"
                     /translation="MCRSIKTLRDGAVVATDDEIEAAALQFVRKISGFRAPAPHNAEA
                     FERAVAQIAESSRTLLGSLQIRGAASR"
     gene            complement(16857..18278)
                     /locus_tag="VM841_03620"
     CDS             complement(16857..18278)
                     /locus_tag="VM841_03620"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HVL89306.1"
                     /translation="MRIRFVAAALSCAMLASLGGSGAARASGVQPSPTVSQPVVEGPI
                     PHGVGMRSGRPYGESLLPLPAGWVEEEFFYGGTAYSGPNKTGTSAAYTSRFLVRRPAN
                     AQDFNGTLVFEWNNVTIPHDHDATWQNYWQTVFSRGYAYISVAAQLLSIEASPIALKQ
                     WDPVRYQSLSHPGDTYSFDIYQQAAEAALDSAVLGDLRPLVQRRIAMGASQSAGRLNT
                     YINGWHEDSGVFDGFQPQVSGAGSVRRDVAPVLWLNSQSEIGTSQVAPDSGQFRLWEL
                     VGPAHTTGYSNNYRWAMLAYAHSNGSANIFDSDDAGAWGYQLPPGSCGIYNHFFAGPI
                     WGAALVALDDWIRTGIAPAPQPRAARDANGRVYDDHGNMKGGVRSALLDAPIATYYAG
                     GVPATGEPCAAGGAAPLTGVTKVFDAATLAALYPTPGAYLTQFEASLDAMLAAGTLLP
                     EGKAEYMRRAGMANVGGALNSSL"
     gene            complement(18293..19723)
                     /locus_tag="VM841_03625"
     CDS             complement(18293..19723)
                     /locus_tag="VM841_03625"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HVL89307.1"
                     /translation="MPSAAVPAIRLIASALALALTLGVAAPGSTSPSWPVQPSAIVAQ
                     PVVEGPIPHGVGIRTGKPYRTSLVALANGYVEEEFFFRGTAASTPTGARTTPYASRIL
                     VRRPADPAAFNGTVLIDWTNVTVPDDTDVSWLPMHRTIMTRGYVYVAVAAQLLAIEAS
                     PLALKQWDPVRYETLSHPGDTYSFDIFSQAAEAVLDPKVLGSLRPKVERRLALGASQS
                     GGRLNTYINTWQEHHGVFDGFQPQVSGAGSVRRDVAPVLWFNSSSEIGNTDVPADSGL
                     FRLWEVAGPAHTTNEYSSYENAVYVYSHSNGVSGDPYDEEAHGNWGSQLIAGTCNNRN
                     YYPLSYVASAALVALDNWVRTGVAPAPKPRALRVNGARAYDEHGNLLGGVRSPVIDVP
                     IATYYGGGASNPTPCSQAGGRLPLTGMTEVFDGAKLAALYPTPGDYLSRLQAAVDAAL
                     AAGNIVPEGAADLMRRAATYGLPKIG"
     gene            19831..21036
                     /locus_tag="VM841_03630"
     CDS             19831..21036
                     /locus_tag="VM841_03630"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL89308.1"
                     /translation="MIKAEPEAPVWTVRAPKGWAPVAEVCRTVFDDIAAVTLRINGII
                     ERSIRDFAPSESPFTHEDRFRSSRGMIAGFLQAVAENRDIHERDIAFAHLVGQQSALK
                     GFPLPPLIDSLYTAYREVWAELVAAAGRAPGTADELLREAGATIWERMHALTTAVTAG
                     YHRQMPRPEALEIRIAGQFVKAIATDPASEEARSLAIDLGFNAEGSFRVAAIAEPAAA
                     VEASRRIARAAQMAGAVAIAAARGGASVLVVQGMREDDLAALIAREASEAPAGMSLTA
                     ESIGGIGAALTQAECALRLAGHGGGLRRFADEWFLAAPLIMREDMERLLRPAIELCRS
                     KPHLASAVRAFSAHGFSLAAAAGELAVTQSTVRYRLKRWHAITAWDPWTFHGLANSIL
                     AFELAGTSR"
     gene            21267..22400
                     /locus_tag="VM841_03635"
     CDS             21267..22400
                     /locus_tag="VM841_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HVL89309.1"
                     /translation="MVEKQADPKTLAEWKHHWRDESDAAFLYGVLADVETDERRREFF
                     RRFVELETRHVEAWADLLRRNGVEVGKLAKPTARTRLTAWLARRFGPAFLLNMLHRQE
                     GQEVRTYLKLHESSPAGRAKDTALTLAQESAGHAEDLRKMSSGDGESWHRTGAGGMLR
                     NVVYGFNDGLTANFGLVAGVVGASASTSDHHNVLVAGIAGVVAGAMSMASSGYLAAKS
                     EQEVHDHEIALERQEIELMPEAEEEELALIYEARGIEPERARKLAADVMRDPERALEE
                     KVREELGIGEPHTTPVKEGALTGVATAIGSFLPVFPFMVFDGVTAIWVSFVVAMASHF
                     GVGGMRSVFTGRGLIRSGIDMFIVGLGVAVAGYFVGEWITELL"
     gene            complement(22384..23172)
                     /locus_tag="VM841_03640"
     CDS             complement(22384..23172)
                     /locus_tag="VM841_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HVL89310.1"
                     /translation="MVDLTDPKQNMDAFLRVRSSLDGSDSVTWFKGTTHAWLPGERGR
                     PLFGFEGYNVSRVVEADGGYDLLTREAVFYTDLRTGEILRTFDNPLNQESVDVLHIWN
                     DPVNQQMRLQGHYGPWNVPVSQAGDDLFFNVDVFLAYPSPLPRAEFPENSQSDLYQAA
                     ELFQFFCKRSHVDDESNPDVPSQVSWTRLGPWLPWMWMGDRPGMLVYHGRGAKLAGGY
                     TDLPPLVRDEIEAGGPQFAEAPREFTQPNETSWTYFRKRATAAR"
     gene            complement(23172..23615)
                     /locus_tag="VM841_03645"
     CDS             complement(23172..23615)
                     /locus_tag="VM841_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3598 domain-containing protein"
                     /protein_id="HVL89311.1"
                     /translation="MGIKDEMPLLARHEGEWEGTYTVIARDGTVTDRHRSHLTCSFPE
                     SGDPPYYQVNDYTWDDGRTETIEFPAAYKDRKIWFDTDRINGHAWEVDDRTIVLHWVY
                     VQSPGIYLYEMISLDDAAQNRSRTWHWFKDGVLFQRSIIDETRVV"
     gene            complement(23688..25103)
                     /locus_tag="VM841_03650"
     CDS             complement(23688..25103)
                     /locus_tag="VM841_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL89312.1"
                     /translation="MAIDVRNPRTGRSDYSFEPPSTDDLDELALRLRDGQAVWEAGGL
                     DVRTAALNGWRDAIGMARGEITAALMADTGRRVESEMEVDAALRMIERWCSLAPDLLA
                     GTERRPASIPGIDLEQGMRPYPVAGVISPWNFPLLLALIDAVPALVAGCAVVIKPSEI
                     APRFIEPLAATVATVPAMENVVAIVAGDGSAGEAIVDRSDVVCFTGSVATGTKIAARA
                     AKRFIPAFLELGGKDPAIVLPSADLDRASSAILWGSTANAGQSCQSIERVYVHESVFD
                     DFVDRLVDKAKSVRLAVPEPDAGDIGPIIAAKQIEVIAAHLQDALARGATAHTGGEIE
                     EHGGGSYLKPTVLTGVTHEMKVMTEETFGPVIPVMSFTDADEAVRLANDTRYGLSGAV
                     FGDADEALGVARRIDAGGISINDAALTALVYDGEKSSFNFSGMGGSRMGPAALRRFGR
                     RQAFLISDAQRPDPWWYPHLR"
     gene            complement(25111..26139)
                     /locus_tag="VM841_03655"
     CDS             complement(25111..26139)
                     /locus_tag="VM841_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVL89313.1"
                     /translation="MEESYRALALQARCRALSGVDDPAQGRIIIRAAIARIGKQVAAS
                     VAFTGPDTRLVVLPEYVLTGHPAGEAFDQWRECVAVDKDGAEEEALGAIAQANTLYLA
                     ANLYERDPAFPDLYFQSCLIFDPSGALALRYRRLNSFMTPTPHDVLDRYLEVYGADSL
                     FPVLRTEIGTLACIASDEILFPEVARCLAMRGAEVFCHSTSEASSPQATIKDVAKRAR
                     AIENMAFVVSANTGGIEGSPIPGDSTDGKSQIVDYEGRVLIEAGPGESMVAAAPVELA
                     SLRRARRRPGMGNMLARSRFDLYASSYADAKHFAPNGLGDGKEPGRGWFLDAQKAVIE
                     RLDGEGII"
     gene            26187..26993
                     /locus_tag="VM841_03660"
     CDS             26187..26993
                     /locus_tag="VM841_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL89314.1"
                     /translation="MGAGKGDRVIDTPVDIRVRDRVAFLTLSRPEARNAIDEAMLEAL
                     PPALRRVRDDPDVKALVITGAGDAFCIGLDIDLLERAFDDPEYFRDVLERFKRILGDI
                     EALPVPVVAAANGLTRAGGFELMLSCDLAVVAAEARIADHHLAFGIVPGGGSTQRLPR
                     KIGEQRAREIIFTGRWLTGDEAAHYGLALHAAPLTRLGDAVEDVVAPLRHRSRTALAA
                     TKAAMNEGASLPLVQALGVETDVFMRYLTNDPHAAEGFRAYREGRDPQWP"
     gene            26984..27439
                     /locus_tag="VM841_03665"
     CDS             26984..27439
                     /locus_tag="VM841_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HVL89315.1"
                     /translation="MALEISSFEAFAPGTSFTTPSLHIDESDAAELIRIGGYVHPLFT
                     DPTYAADSVFGRSPLPGEAVLHAMGGLAERSGRFDESVVALLGFDAVRFHAPAFPGDT
                     LHMSVEVIRREARPNGKRGEVVMAWRCRNERGELLCDATARMLFRLPHA"
     gene            complement(27430..28011)
                     /locus_tag="VM841_03670"
     CDS             complement(27430..28011)
                     /locus_tag="VM841_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89316.1"
                     /translation="MFVQIIRAKVADADGINSAWQEQRDATAEGFLGATGGITTKGEL
                     LIAARFENRELAMKNSASEEQTARWNQLEKMLAGPAEFLESEDVDMPKEGSGDAGFVQ
                     MMRATVKDRKAYEAIEEKFDQFAGLRPDLIGHMTVWLPGNRIHIVDWFTTEAEARAGE
                     KKEMPAEMQALFAEWQSLCSDVEWFDISAPLYA"
     gene            complement(28245..28481)
                     /locus_tag="VM841_03675"
                     /pseudo
     CDS             complement(28245..28481)
                     /locus_tag="VM841_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            28595..29605
                     /locus_tag="VM841_03680"
     CDS             28595..29605
                     /locus_tag="VM841_03680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL89317.1"
                     /translation="MQLSYGPWGASLDEVVAASKKAEDAGFSRVWFAELHRSAFVPAA
                     AVATATTRIGVGTGIALAFVRSPMTTALEAMDTDELAGGRFVLGIGSGVQRLNESWHN
                     VRFGKAVGHTRETVAIIRRFIAESHRGGSIEVDGEYERMRLRGYQRPFPPQREQIPVY
                     LAAVGDAMTRLAGEIGDGWLGHELGSPRYLASAILPNLQAGLSRAGRDRRSLDVVASA
                     CCAIDGDGRLAKRWMAGLVAFYATVRTYGEFFAFHGFAAEAAAVQERFRAGDEAGMVE
                     AVPDEMVDAFALAGTPDEVRAKLKEYDGIADGIKLSPPTHLVPAEVTRHCQATILEQL
                     AP"
     gene            29602..30699
                     /locus_tag="VM841_03685"
     CDS             29602..30699
                     /locus_tag="VM841_03685"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL89318.1"
                     /translation="MNAIKPASFPWFEYARYTFSLGLEHAGRVWLSGHSASEYDAREE
                     RIVVKGGMEQQTRTAYAKISAILEAAGLTLGDATRVVEYVTAKGMDHYAEARAVREEL
                     FGSAQPAVCTVVVNSLLRPAAFIEIEVTAGGGEDDGIVYLPSIAAPEAGEDLVAQTEA
                     IFDKAAALLADAGLGMDSIVKTVDYTTPRTLAGYKRTGRVRKDRLGPVYPAAAGILMS
                     RLQTPGTLVSMDITASRHQSETVNPGWERYAKLTYSPAVKAGSMLFMSGQAALDPATE
                     QAVHTGDVAAQAEYTYQNIIEVLKAAGAGPENLVKTIEYVTPAGLKRYREVAAVREQL
                     LKQPYPASTGCICETLLRPEFEIEIDPLAIL"
     gene            complement(30747..31571)
                     /locus_tag="VM841_03690"
     CDS             complement(30747..31571)
                     /locus_tag="VM841_03690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL89319.1"
                     /translation="MSVEGSTWSAAFAGASRDAMQVYDRVIARLFEPWARDLVGRLDP
                     DPGEAALDVACGPGTVAVLLAQAVGPAGRVVATDISPAMIALGREKDLPSGLAPIEWM
                     EAPAAPLPVSDASFNVITCQQGLQFFPDPVASLAEMRRALRPGGRAGVATWTHVSEQA
                     FHFLYEAIREVLGQEMATRYTGPFSFYGDAAASAAEAAGFESVTARTVTLPAIIEEGF
                     PGAFDTLVASGIAAELAELDEATAIRLREVAARHAEPLVEDGTIKASMTATVMILG"
     gene            31952..33325
                     /locus_tag="VM841_03695"
     CDS             31952..33325
                     /locus_tag="VM841_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVL89320.1"
                     /translation="MTAADISAALVALTIVIGSAHAIGQVMERLRQPRLIGEILAGVL
                     LGPFVFGRVAPAVTRTVFGGDAADDTTRIVIEFVSWLGLLLLMFLSGSEARRVLAREN
                     RRATAWLFGLGTPLPFVAALIVGSWISLDGLAGPSGSRASVLLILAAAATVMSIPVIS
                     RIFHDLGILGTRFASLVLSVAIAEDIVLWAVLAIATALAASGAGAAGGIATHVAATVA
                     YLFAGVAVAPALVKRLHDARWNSLATASPVRYAVLLLLVYGSLASLMNVNLVFAAFLA
                     GFGLVGGREGASRERFAVPLEAITSVSTAVFVPVYFVVVGSQLQLGHQFAPVMLAVFL
                     IGSTIVRAASVAGAAKLAGFAPLDRINLAVTCNARGGPGIVLASIAFEAGIINGRFFT
                     TLVLTAILTSQFAGAWLAWVLRRGRPLLSDDHAGPERHEASASPLSGQGRGPQRTAVE
                     RRRRRQR"
     gene            33421..33813
                     /locus_tag="VM841_03700"
     CDS             33421..33813
                     /locus_tag="VM841_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89321.1"
                     /translation="MSEDLAAAYWQEFAERNALRTGTKEQKLEYQTRVFPVEDGIATR
                     IDDDDPALLADMISIAEAAPDDESRGMFGAGILWEYLYNTSDKNAAAIADAAKNHGAF
                     LDALRAAFAGWDGLGSSMKQNLKPLLDG"
     gene            33821..35278
                     /locus_tag="VM841_03705"
     CDS             33821..35278
                     /locus_tag="VM841_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVL89322.1"
                     /translation="MDLLTPVGGIAAVLVGAALVAGLVERGPISFPIIFLGAGLILGD
                     GVTGAVHIDLHAPILEIVAFVTLALVLFLDAVRLETKEMRRDWLIPALTLGPGTISVI
                     ALVATAGVVLLDLSWTVAFIAGAALASTDAVVLRDVLRDKRIPGPVRRSLSIEAGTND
                     VIVLPPVLILATVAAAKAATAADWAWFGVQLFLIGPAIGAAVGAGGAWAMSRVDRRTP
                     VRTEYQALYGVGLVLTAFTLGEWLGADGFLASFAAGAAVALTNNDLCECFLEFGEVVA
                     EMAMLLTFVLFGAVLSPMLGETVGPPVLIFAAFTIFVARPLPLLSVLSIRHTELSHPA
                     RAFIAWFGPRGLNSLLLALIAVGAGVPNGERLFAIIGVVVVTSVVLHGVSATPLSAWY
                     GRSVERTTQPEERHATATGLFIADPASQQRISVDALATLLASADPPIVLDVRSRSAFE
                     QSDAQIPGSIRLRLNEIDAWAEHQTKDRLVVLYCT"
     gene            complement(35451..37484)
                     /locus_tag="VM841_03710"
     CDS             complement(35451..37484)
                     /locus_tag="VM841_03710"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVL89323.1"
                     /translation="MRRFPLLLAASILAAGLLPAQAAAPVSFPSLVPSWAVASARVAR
                     VDPAREVRVELILPWRDPAAVSRLVRAVSDPASPSYRAYLTSEKFRARFAPASATVDA
                     VVSWLRSQGLVVGRMPLNRVVVPARGTAAQIERVFGVTLSTYRVGGMTLRAPDRAPSL
                     PAHLAGLGLTIRGLDEASALFRPSRVEDPPVVPETPANPADARPPSYVAYAAPCSAYD
                     GATLDRGAPPAYGRTNPAVRCSVSVAGLRKAYGADTLHARKVDGRGQTIVMIGSHAIR
                     SLPSDIDTWSKRNRIPALRPEQFRQFSYPGAYETPNDPTGSFLRPAVWAVQAAMLFET
                     MRAIAPGADLRYIGSTSSMDLPNATLLAVDQHYGNVIVNGWYAVSEALVTPGDTAPID
                     RAAEQAAATGISLLFASGDLGDNSYFNAYETGELEVFSGEPDVVVPNVPVIPAHPSVS
                     FPAHNPLVTAVGATSLILGRGDAYLGEMGWAKTHRVLRDGAWSDPSAGSFRGSGGGRS
                     AIYGQPDYQRRSVPYLLSQRSDGTHGRTVPDIAVNGDAETGLMIGYTQTYPDGTVKYA
                     ERRINADTASTALFGAMLTLVNQRSGKAAGFVNPALYALSRTKPAAFRDIVEIPGVKA
                     EVRRQWVNATDASQGQTVVLKTYQEFLSNPPRRGYDTNTGLGSPNRTFLSVLS"
     gene            complement(37525..37992)
                     /locus_tag="VM841_03715"
     CDS             complement(37525..37992)
                     /locus_tag="VM841_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL89324.1"
                     /translation="MQVKELGHVVLYVRDIERSVRFYRDVLGWRQILPDPNEPSRIPV
                     AAFSADNGRTHHELLLLEVGQNAAPLPEGRRVGMYHFGLKVGDSDDELREVVKKLQDN
                     DVPIQGASDHTVTHSLYIADPDGNEIELYIDVPGVDWRSDPSLIGSPIKPLRL"
     gene            complement(37996..38544)
                     /locus_tag="VM841_03720"
     CDS             complement(37996..38544)
                     /locus_tag="VM841_03720"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVL89325.1"
                     /translation="MKRCEWAEGGSELMVAYHDDEWGVPSRDDVHLFEMLTLEGAQAG
                     LSWNTILNKRQGYRAAFAGFDAVAVARFGKRDVDRLMKNPGIVRNRSKIESTISNANA
                     ILALEEPLSDFVWSFTKGKPVVNRFERLDELPAKTEVSIAMSKALAKRGFRFVGPTIC
                     YAFMQAVGIVDDHTIVCFRRRR"
     gene            complement(38582..38926)
                     /locus_tag="VM841_03725"
     CDS             complement(38582..38926)
                     /locus_tag="VM841_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89326.1"
                     /translation="MIYLRYRMRLARRARENMKEFWSWLEAREQWFYRDLPMVKEVRW
                     YYSIVGGVYVLENWAAFEDEAAWGAYRAALGTLKADEGWESERVSQEDWWEFLDTAMF
                     TDPPVPVGFRRD"
     gene            complement(38923..39552)
                     /locus_tag="VM841_03730"
     CDS             complement(38923..39552)
                     /locus_tag="VM841_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HVL89327.1"
                     /translation="MITPESVSGWTEFPIPGGTAPVPIVRLRSDETSRAQTLFVRFPA
                     GWSRPVSGFYEAAEELVVIRGVLHMSGVTYRAGDWGYVPAGSVRSWTEAAEECLVFAR
                     FDGPARWRETSESGAPTPMVKTMFGSLHDDAAHTVEPSPLGNRLARRLRRGERDAAWL
                     AESVEAGAPAAVTAELFDLTQAAYAFVVAGDPLPACGGPCFVRTFGGEV"
     gene            complement(39549..40259)
                     /locus_tag="VM841_03735"
     CDS             complement(39549..40259)
                     /locus_tag="VM841_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HVL89328.1"
                     /translation="MSERDEPGLWQSLMRDSAEIHRAAERRPFMVVFFKAELPKDAYI
                     EYLGRLSYIYAALEETSEALKDDSVVGRMHTPELHRSAAIDADMRFLAGEDWRDRVKP
                     SPQTESYVAAIRSTIDRFPPAFAPHQWLRYLGNVLAQRINLRIMNKAYGFDEQGTAFF
                     RFDRIEDPRGFLKSYHEKMNSMPLDDDQIKRCVDEVNGAWQHQIDFTDELAADFGLRH
                     VSVEEAEQLMSDLAAHHP"
     gene            complement(40337..41083)
                     /locus_tag="VM841_03740"
     CDS             complement(40337..41083)
                     /locus_tag="VM841_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HVL89329.1"
                     /translation="MLAFPASQGEQVMSKILKRVEEASTRLGEKVDEAIATKPFSRVM
                     RRESWGDHQRAEYSPFEQSLVKATVSKEGYADLLAQTWYIYDALETKTEQMLDDPVAS
                     RVIFKDLYRREHVAADAAFFAGPDWKGTYPILPVVQEYCDRINNASPAQFVAHHYVRY
                     MADLSGGLFIAAALRDAWKLNGEGLTYYDFAEIGDAAEFKQQYRSILDDLPVDAATKK
                     LIIEEVMVAYEYNIELATLLGDKHLVAAQA"
     gene            complement(41173..41946)
                     /locus_tag="VM841_03745"
     CDS             complement(41173..41946)
                     /locus_tag="VM841_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVL89330.1"
                     /translation="MAREAQGQTIVAIERAADVLALFADADEPTLGVTEIAQALNLSK
                     AVVYRILSSFRAKGFIELDEASRRYTLGPRALHLGLAFVNRTEVPRVARPVLEALSRE
                     THETATLSLRTGDRRVYVDQVTPDRDVKMVVQLGLSVPLHAGASSKAFLAYLDEDEQT
                     DYLTEPLERLTGLTITSRSALAKELKDIRDRGYAMSLGERMEGAGSVAAPVFSHDGKP
                     AAVISVCGPLERFRGEATDLAKKLLDATDRLSRRLGYRP"
     gene            complement(42180..43610)
                     /locus_tag="VM841_03750"
     CDS             complement(42180..43610)
                     /locus_tag="VM841_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HVL89331.1"
                     /translation="MARYDVVVTGGTLVIPFVGEVRGDIAIKDGRIASIAGDLGPGDA
                     DEAIDARGKHVFPGAVDSHYHLGIYRGLTEDTESETTSSLVGGVTTVISYFRTGSHYL
                     NKTGPYRQIFPEVLAAVDGHAKVDYGFHLAPMTAEHIAEIPWLVSEMGVASFKYYMFY
                     KGLNLSADSRDAKGYTMSEEYDLGHLFEIMEAVSAADLAHDGRISLSLHCEQAELLRV
                     FIERAKQDPSVRGLAEYAAGRPPLTEQLSIEEAGVLADATHVRTNFLHLSSGEALDKA
                     NEVRHRYGLDANLEVTVHHLALNVAMLEGKGLGGKVNPPIRDAEDNEALWQGLADGRI
                     GTVASDHACCMEESKGEDLWPALPGFGGTALLYPVLISEGHHKRGIPLDRIAQVASAA
                     PARTYNLYPRKGTIAVGADADLAIIDVGLEQIVTPELCRSAQDHTPFSGVAVRGWPAA
                     TLVRGIPMYRQGEIANDFPGLYLRRT"
     gene            complement(43586..44962)
                     /locus_tag="VM841_03755"
     CDS             complement(43586..44962)
                     /locus_tag="VM841_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVL89332.1"
                     /translation="MSTAAARLGAFAAAPAVPAGVLAAVRLHVLDTVGVAVAALPLDT
                     SQSLIAMLREQGGAAQSSVFGGGERLPMTSAAFANGVLAHSLDYDDTHLPSILHPSAS
                     VVPAAFAVAERVGASRDAFADAVAAGIEICIRTGMAGYDTARRQSMFFERGQHATSIC
                     GALGAAAASARLLGLDADGIAHALGIAVSMGSGVLEGNRAGGTVKRLHCGWAAQAGVT
                     AALLASRGITGAPTAFEGRFGFYQAWLSGEFDEAPLHAPGEWEIRRLHFKPYPANHFT
                     HAAIDAAIALRDAGLKPEDVESITVGVAAPAVRTIGEPINAKRAPATGYQAQFSGPYA
                     VAAALHGGGGLGLGLDDFTDALAADPDRRALMQRIEIVADPACDAIYPDELPAIVRVR
                     TRDGRDLEQKVLQNRGGPGRPLSEDEVARKFRDNASRVLSEGGADAVERALREGEVTV
                     PWPATTSS"
     gene            complement(44959..45759)
                     /locus_tag="VM841_03760"
     CDS             complement(44959..45759)
                     /locus_tag="VM841_03760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020112694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVL89333.1"
                     /translation="MPDPLLDAVARGVRIFDLGREMFAGMPQSPNHPEFRMALVRRHG
                     DMKRADGGSAANEMIVTGGHVGTHIDALAHVSHDGKLFGGHDAAEASVGGRFSVHGIE
                     TVAPWVCRGVLLDVPRALGIDACDPAYEVTAADLEACEVSLREGDVALIRTGWGRRWP
                     DADAYVGRATGVPGPGEEAAKWLASRGIRAAGADSIAFERLPAGAGHALLPAHRVLLV
                     EHGIHIMETLALEELAAADVHEFVFVLAPLKIVGGTGSPVRPLAVVGA"
     gene            complement(45752..>46413)
                     /locus_tag="VM841_03765"
     CDS             complement(45752..>46413)
                     /locus_tag="VM841_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVL89334.1"
                     /translation="VMMALYHRDARGGTGQQIDISILEPIVTVLGPQPIVYDQLGEVQ
                     PRRGNRSDNNAPRNVYKTKDGTWVAISTSADTIARRVMELVGHPEVCDEPWFTTGVGR
                     ARHGDLLDGYVAAWIAERTRDEVIDAFEAAQAAIAPVYDVAGLMDDPHVRATEMITPV
                     PDEDFGSVRMQNLLFRMSDTPGSIRWTGRALGADTDEVLGSIGVPAERIAELRERGVV
                     A"
BASE COUNT         7199 a        16134 c        15741 g         7339 t
ORIGIN      
        1 cttcctggcg atcccgtaca tggtctggcg agcgctcggc cgccgcgatc agaacgcggc
       61 gctgctgctg atcctgttcg cgccgctgta tctgccgtgg ctggtcacga cgcggccgtt
      121 gttcctgttc tacatgacac ctgcggtgcc gatcctggcg ttgatggtcg cccacatggt
      181 cgaccgcatc accgaggaga tgcgggcatg gcagctgacc gtgatgttcg cgttgatgtt
      241 cacgctgccg ttcgtgccgc tctacatcca atggctgcac gcgggccgca cctcgcacct
      301 gctctacaag atcccggcgc tcgcggtgat cttcctggtc gccaacggca tcgcgaccct
      361 gctcgcctac acgcccaagc cacggcggct gatcctggtc tacggcgggc tggtgctggc
      421 gatgttcgcc tacttctacc cggtgctcgc cgcgggccac atccccgacg gcggcacctt
      481 cgggtggcgc aagcacatgt ggctgcagcg cgactgcggc cagcagggca tcttgctgac
      541 ctgctggatc taggtcgccg tgaaggtctg gtgatccggg gaccggcggg ggcgcgtcgc
      601 tcgctgcacg gctctgacct gcaaaaaggc cctagaaacg gatcggggcc ggaaacccgg
      661 cccgatccgt cctggcgaga ggactggccc ctatttcgcc gcccccttgc ccgattcctc
      721 ccatccgggc ggaaatttgg ggaggaatct ttcgcgagat cctcgaatcc gtttccatga
      781 cctcacaccg cgcattccgt gctgccgtgc tcctcttgat ggcctccttt ttcgccatgc
      841 cgctcaaggc ccaggccgac ccgtgcgcgg ccgtgctgtg cgccggagcg gccaccgccg
      901 acatcacgcc gccggtcacc acgccgcaat ggggctacac caaccggcag ggcctgctgt
      961 tctcggcctc cggggtccag tcccacatca ccggcggcaa cccggcgggc gtggcccagg
     1021 aacggttcgt tcgcgacaag accaacggcg agatcgagct ctacaacaag gagttcgtcg
     1081 cgagccgcgg catccatctg cggctgcagg ccaacgcctt cgtcattcag ggcgccaacg
     1141 ggcagcgggt cgcggtcgtg caggccgacc tcggcggcat cccgggcgag ctgcataagg
     1201 ccgtcgccaa acgcatcgcc gcggtcacgg gcatcgccga gtcgcacctg ctgatcagcg
     1261 cgacccacac gcacggaggg ccgggcggct tcttccagta ccagggctac gcgctgctcg
     1321 gcggcgacga gtacgaccca cgcgtgttcg atgcggtcgc aacgggtctg accaacgcgg
     1381 tcgtgaccgc ctacaacatg ctcgcgcccg ccaagatggc ctcgacccgg atcgccgcgc
     1441 cgggcgcgaa ccgcaaccgc cgcgtggcgc agtggtgccg caaccccgag gcgctgtgca
     1501 acggcaccac gcccacaccc gcctcgccgc ccgcgcacga cgacgacacc acggtattcc
     1561 gcatcgacac cgcgaccggc ctgccgctgg gcgtgctcac gcagttccca gcgcacggca
     1621 cggtcggcgg cgacgggaac ctgctgttct cgggcgacaa ccagggctgg gcgacgcgca
     1681 aggtcgtcga aggcattcgc gccgcctccg cgcctttgcc ggacggtcac gtgatcgtca
     1741 acgcgctcgt caacggcgcg cagggcgacg tgtcggccgt gggttcgggc ttcaaccagt
     1801 acgcgtcgat ggaggacgcc ggcatgcggc aggccgcgcc ggtcctgtcg gcctgggccg
     1861 cgctcaagcc ggcgctgcgc accgacgtga cggtggacgc gcgcttcgag catgtctgct
     1921 tctgcggcca aaggatcgac gacgagtttc tctacgacgg gccggcgatc gacaagcgcg
     1981 acccgatgtg ggatcgcgtc gccccctacg cctcgctagg cgcgacgatc gtcaacggcc
     2041 cgaggaccac gccggttccc acgcccgcgc agggcaacaa gaccccgggc ctggtgggcg
     2101 cgggcctggt gccctcgatc gtgcgcctgc agacgcttcg agtcggtgac gtgctcttcg
     2161 cctcgattcc gggcgaggcc aacgtgcagg tcggtcggcg catcgtgaac cggctgatgt
     2221 cgctgccggg ccgtccgtgg agcacggtcg tgatcggcgg gctcggcaac gactacatct
     2281 cctacttcgt cacgcccgag gagtacaccg cgcaggagta cgagggcggc ttcacgctgt
     2341 tcggcccgca gtcctcgccg ctgatggagc aagagctgac gcggctcgcc cgcgcgatga
     2401 tcgcgggcgt gccggtcgcg ccctgcgatc cgtccgtgga cgcgggcgac tgcctcgagc
     2461 atctcgcgca tcccgacacc accgcggcgg ccgtcacgcc cgtcgcgccc gacctgggcg
     2521 gcagcgcggc cgtggtagcc gcggccaccg gcaccgcgcg gctgtcgcac gcgtcgatgg
     2581 tctggcacgg cggcggaccc accgccgagt ggcggcccgg cgccgaccgc gtcgcgatcg
     2641 agcggctcga cggatccgag tgggtcgacg tcgccggtga cgcggccggc ggcgctacca
     2701 cgatccgcta cgagaagatc gacggcaagc acacctggat gacggtctgg gacgtgcccg
     2761 cggacgccgc ggccggccgc taccgcttcc gcgtcgaagg ccgcacggcg acctcgccgt
     2821 tcacgttctc gccgtatctg ctgacctccg agttcgacgt gctcggctcg aacgcgatgg
     2881 cggtgacggt gacgaagggc tcgtcgtgga cggttcgcgc gacctacccg gtcgatcccg
     2941 cggtctcgtt ccgtatccgt ccgctcgtcg ccgggaccgc gacgctggtc gtggtgcgcg
     3001 gcggcgtcga gacgacgctg acggcggcca tgagcggagg ggtggcgaac ttcgcgctcg
     3061 aggccgggga cacgctggtg ggcgcgagcg tcgtggatgc gcacggcaac acgggtgccg
     3121 catgagacgg cgtctgctgg ccgcgctgct ggcgctcgcc gcgatcgcac cggcaaacgc
     3181 gtcgcgcgcc gcgaccgtgg tggccgacga ggacgcgttc gtccggttga acggcagctt
     3241 cctcgtgatc ggcaacgatc tgatcgaacg cttttggacc ctcggcggcg agacggtttc
     3301 ggtgactacg cacaaagggg cgttcaccgg cggctggtcg tcggatacgc agttcggcac
     3361 cggcgcaccg gatctgtgga ggatccgggc gtggtcggcg tcgcggcggc ctgccggcgg
     3421 cgtcgagctg cgcatgcgca tggccggcgc gcgtccctac gagatcgagc gcatcgcgcg
     3481 cgtgcttccg ggcgtgagcg cggtgcgcat ggagacgatc gtgcatgccg gcccggcgcc
     3541 ggtcgtgctg cccggctaca cgttggaatc gctgtcgaac ccgctcagcg cgcccgccgc
     3601 gcgcgcgatc aacttcagcg gcggcgcgga ctggcggccc gcctacgact atcgccgcga
     3661 caccgtgggg ccgcagttcg acgcaaacgc cgagtggctc gagaccgccg acggcgggct
     3721 cggcctgctg atgcagcgcc gcaactatct gtcctcgcgc atgcaatggt ccgggcatcg
     3781 ggcgcgggcg accgtggacc tgtcgcacga caaggcatac ctcggcccgt tcgaggagat
     3841 ccgccccgag ggcgggcatc cgcccgccgc gcccgcacgc acgcgaacgg tcgagccgtt
     3901 ccgcccgctc gcattggagc cggtgatcgt ggcgatgggc accggtcccg acgatctgac
     3961 ctggcaggcg caccggatgc tgatgagcga cgtcgccccg tatccgcgca cgatcaactt
     4021 caacaccgac cgggtgcgcg gcgcgccgtt cgagctcggc gcgcgcgacg gcgtgggact
     4081 gccgcagttc aaccagctct ggcccgttgc gaaggcgatg ggcgtcgaaa cgttcgtgct
     4141 cgacgacggc tggcagctct acaacggcga ctggctcgag ccggacgccg cgcgctggcc
     4201 caacgggctc gaagagccgc gcgacatctt ggaggccaac gggatgcggc tgggcctgtg
     4261 gatggcgccc ggctcgttcc atccgcactc gaatgcatgg aaggccgatc ccgaggcgac
     4321 ctgcctgccg gtcggcgccg gcaccgccgc ctacaacgcc gcgtcaccca acaccggcag
     4381 caacgcggcc gggatcgggg tgtgggactt caacgcgccg ctcgccggcg agcttcgccg
     4441 ataccgcgac aagctcgcct ccgagatcgc gacgctgagg gcgacgttgc gcccgaccga
     4501 gttcaagttc gacttcctgg tatggctcga ctgcgcgttc ggcacgatgt acgagcagcg
     4561 cgacgcgttc ttcgacgaga ttgccgccct gtccggcacc ttcccgggaa ccggcttcgg
     4621 catggacgag accaacgact atcgcagctt cccgtacgag tcgatcctgt acgggccgac
     4681 ctggttccaa aacggcaacc catcgcagga tgccgtgctg cacaacgtgt ggaccatggc
     4741 cccgtacgtg cccggcttca ccatcggcca ggccgccacg attcgtccgg gcgacccggc
     4801 ggccgtgatc gacgagaaga tggcggccgc gctgacgtat cacatgacct tctggaccga
     4861 catccgcgag ctggcgggcg cgaccgcggt caccgaccgc gtgcgcgtgt ggaccgactt
     4921 cgccaaacag caccggatga cgaacggctt cgcgtatccg ctgctgtcgg atcccctggg
     4981 ccgggcgtcg tggagcggca tgcagccgtg ggacgccgag acgcacaaag gcatggcact
     5041 cctgttccgc ttcgactcgc cgaacgcgac gcagacggtt tcgctgcgtg ggttgtctgc
     5101 gtcgacgacc taccgcctga ccgacgtgac gccgaccgag gcgtcgttcg tcgtgggcga
     5161 gttcagcggc gcggatctga tggacggcat tccggtcacc atcgcgcaga cccgcggcgt
     5221 gcgtgtgctg acgatcgatc ccgcctaact tttcccctgt gcacggctca aggctcaacc
     5281 accgatcggg gtcttcgcta cgcgcggcgc atcacaaagt cgaggacgat cggcacgatc
     5341 atcatcgcgg cgaaggcggc ggcgcgcagg cccttcagcg gcttgggcgg gcgcaggccc
     5401 gctcccagca cgtcgccggc atgcggggct cgcaccacgc cccacggcgc gagcgcggcc
     5461 tcggcctgaa cgccgttgcg ggcgcgcagc atcaggccgc gcgctttgcg ggcgttcgac
     5521 gaccggatcg agcggcgctg cagcaggaag aagccgggcg agcgcttcgg cagctgcatc
     5581 tcgcgggtga agacgatggc catgttcggg atgtcgatct cgttgtacag gaacagcggc
     5641 aatgcggatc cgtcccccga gtacaggtag ccgtgcctgc cgcgcggctc gggcacgggc
     5701 ttcgatccga cggtcgccca gccggcctcg tgctcggccg gctcgggcgc gtccgaattg
     5761 gacgcgtcag gatcgggcgg cggcgtggtt tgcggaagct gatacggcac ctgtgcgggc
     5821 accggctccc acgggttgcg gccgtggcgg tccagcgcgt cggcgaagac cgcctcggga
     5881 agctcgcctt cgatggcgaa acggtggttc ttgttgaagg ggatcatcgg gcggtcgtcg
     5941 agcgcgacga gcttgaccgc ctcgcgcggc acgaccatcg gctgcgacca ttccggcagc
     6001 gagatcgtca tcgtctgcga gccgaccgtg acgacgcagg tctcgcgatc ggcgcgcagc
     6061 cagaagtagg ccgcgaaccc gaaccccacg agcgccaccg tgatgccggc gatctcgggc
     6121 acggccgagt cggaggagcc catgaacgaa aggatgagct cgatcagcgt catcgcccca
     6181 tacgcgatga agccgcccac caggcccccg accgcgaaga gcacggcgat caccttcggg
     6241 ctgcgcgtgc ctcgcggcgg cacgatcggg atcgccccgc cgggcgcgac ggttggcgcg
     6301 gcaggcggcg gcgccggggc cgcgcgcagc ggcaccggca acatcatgtc gtagccgtcg
     6361 ctggtccagg tcattccctt gtgtatcggt cgaaatccgg cggacttgaa cgataggatg
     6421 gcgcccgcca ccaacctttt taggagcgtt catgaacctg caaggaaaga ccgcgctggt
     6481 cacgggcggt gcatcaggcc tgggccgcgc gaccgtcgag acgctgcacg cggccggcgc
     6541 gaacgccgtc attgtcgatc tgccgcatgc ccccggcgac gaactcgtca agaatcttga
     6601 cgagcgtgcc gtcttctccc ctgccgacgt caccaacacc gaagaggtcc aggccggcat
     6661 cacggcggcc gtcgagcact tcggcggcct gcacatcgcg gtcaactgcg cgggcgtcgg
     6721 ttgggccgcg cgcacgttgt ccaaggacgg tccgcacgat ctgggtgtgt tctccaaggt
     6781 cgtcgaggtc aatttgatcg gcacgttcaa cgtcatccgt ctcgcggccg cgcagatggc
     6841 ctcgcaagag ccggccgccg gcgacgagga gcgcggcgtg atcgtcaaca ccgcatcgat
     6901 cgccgccttc gacggccaga tcggccaggc cgcctacagc gcatccaagg gcggcgtggt
     6961 cggcatgacc ctgccgatcg cgcgcgatct ggcacggatg aagattcgcg tctgcacgat
     7021 cgcgcccggc acgttcgaca cgccgatgct ggccgcgctg ccgcaggagc atcgcgacgc
     7081 gctcgcggcc gcgattccga atccgtcgcg cctgggtgat ccggccgagt tcggcgcgct
     7141 cgtcaagcac atctgcgaga acagtttttt gaacgccgaa acgatccgtc tggacggcgc
     7201 gctgcggatg gggccgaagt gatgccggac atcggcgcgc tcctgccgca gatccgcgcg
     7261 gacctggaag agctggtccg catcccgtcg gtcagcgccg acggcttcga cccggcgcac
     7321 gtgcgggcat ccgcgtccaa gaccctcgag ctgttcgaag ccgaagggct ctccaccgag
     7381 ctgctcgaga tcgacggcgc acatcccgcg gtgctggcca cgtttcccgc gcccgccggc
     7441 gcgccgaccg tgctgctcta cgcgcatcac gacgtccagc cgccgggcga ggagtccaag
     7501 tggacgtcgc caccgttcga gcccaccgag cgcgacgggc gcctgtacgg acgcggcacc
     7561 gccgacgaca aggccgggat catggtgcac ctggccgcga tccgcgcatt cgaaggcaca
     7621 ccgcccgtcg ggctgaccat cctgatcgaa ggcgaggagg agaccggcag cgagcatctc
     7681 ggcgcgttcc tgtcggcata cggcgaccgg ctgcgcgcgg atgcgatcgt gctggccgac
     7741 agcggcaatt ggcgcatcgg cgagcccgcg ctcaccacgt cgctgcgcgg cctggtcgat
     7801 tgcgtcgtcg aggtgcgcac gctggatcac gcggtgcaca gcggcatgta cggcgggccg
     7861 atcccggacg cgctcacgtg cttggcccgg ctgctttcga cgctgcacga tcccgacggc
     7921 aacgtcgccg tcggcacgcg agtgcatggc agcgccgatc cgctcgacct gaccgaggac
     7981 gagcttcgcg cgcaggcggg cgtggcggac ggcgtgcgga tgatcggcgg cggcgcgctg
     8041 accgagcggc tgtgggcgcg gccggcgatc agcgtgctcg gcatcgacgc gcccaccgtg
     8101 caggcggcct cgaaccagct cgtgcccgtc gcgcgggcga agatctcgat gcggatcgcg
     8161 cccggcgacg atccgtcgca ggccttggac gagctggtcg cgcatctcga acgcaacgcc
     8221 ccgtggggcg cgaaggtcac cgtccgtcgc ggcagcaccg gccggccgta cgcgctcaag
     8281 gccgacggcc ccatctacga ggcggcccgg caagccatga ccgaagcgtg ggacggcaaa
     8341 aagcccgtcc acatgggcgt gggcggatcc atcccgctcg tcgcagcgtt cgcggatcgg
     8401 tacccggacg cggccatcct gctgaccgga gtcgaggatc ccgacacgcg ggcccacggc
     8461 gagaacgaaa gcctgcacct gggcgagttc gcccgcgcga tccgcgctga gacgctgctg
     8521 ctggaacacc tcggcgcgcg gtagcgacca aaggcacgcc gaaagaacgg ccaagggccg
     8581 tcgggatgca tggtgatcgt ccgcggctcg gcggccggag cgttgcccgc tcacccggcc
     8641 gccattcgtt cgccgccctc ccgatgcatc gacgcccgaa attccgggtt cggcaaacgg
     8701 ggcgaatccg gcaagtgatc ggttgaaggt cccgatcgag gcccactcat aaccggcgga
     8761 gatctcgcgc gttgacttcc gtgacggctc cccgacaata ggaccgctgt gaaacgacat
     8821 ctcgtcatct tcttcgcgat cgtggccgcg ctcggcgcgg tcatgccggc gcgcgcccaa
     8881 tccgaccgcc tcgaggaggc ccggcgcaag atccgccagt accaggacac ctcgctggtt
     8941 gggctgctcc gcgcggccga cgacaagcgg cgagcgatcg aggagtcgat cgctcggatc
     9001 gagcgtgaga tcgtggacgt gcagaagctg atcgacctgt acaagcccga gctcgaacgg
     9061 gccaagatcg agcgcgacct cgcctcgcaa gccgtgatcg cagcgacggg cgcgtacaag
     9121 aacagcaggt tcatgctcgg agccaacgcg ctgtcgatgt acgcgaccgg gcagttgccc
     9181 gagatgttcg tgctcatggg caccggcgac atcagcgact acgtcaccgc gcgcgtctac
     9241 atggacaccg tcgtggactc gagctcgcgc gtcgtggacg gcttccgacg ctcgcgcgac
     9301 cggctcgaga aggaaaaggc ccgcgtcgag acgcagaccc aggcaatcct ggatcgcggt
     9361 accaagctcg aagccgaacg ggcgcgcctg gtgaagctcg cggagtcgaa gcagaagatc
     9421 gcccaggacc tggacgcggc cagcgccgcg cgcgaagagg cgatcgcggc gatcaagtcc
     9481 gatccggccg gcttcgacag catcctcgcc tcgtacggct cggcgaccga ggcgatccgt
     9541 atgctcgtcg tcgcggccca gcgcggacag cccgtccagg atcccgagaa ggccgcggtg
     9601 tgggttccgg tcgatggccg cgtgtcgtcg aagtacggct ggcgcatcca tccgatcttc
     9661 gagaagcgct cgttccacac cggcaccgac atcgccgcac cctacggcgc ggacgtgcgc
     9721 gcggcctcgc ccggcacggt cgtcgacgtg gtctacctcg gcgcgtacgg gctcgtcgtg
     9781 gtcgtcgatc acgggttctc gctcgccacg atgtacgcgc acctcgctcg ctcgctcgtt
     9841 catccgggtg agcgcgtgca ggccggtcag gtgatcggca aggtcggatg caccggctgg
     9901 tgcacgggcc cgcatctgca cttcgaggtc tggagccgcg gaaacccgca gaatccgcag
     9961 gcctgggtct cctagagcgc catccggaac tcaccggcac cgcctttacc gctccgctag
    10021 gctccgcggc gcatgaacat gcgcgcggcg atctcgcagc ggccccacgc ggcccgcgtc
    10081 gcgctcgcgc tcggcctggt gtacatcgtg tggggatcga cctatctggc catccgggtt
    10141 acggtcgaaa cgattccccc gctgctgtcg gccggcgttc gcttcctgac cgccggcgcg
    10201 atcctgtacg cgttcgcgat ccgccgcacg cccaaggacg agcggccgac accgattcat
    10261 tggcgctccg ccgcgatcgt gggaaccgcg ttgctgctcg gcggcaacgg cggggtggtg
    10321 ctcgccgaat cgtggggcgt gcccagcggc atcgcagcgc tcctggtcgg caccgtgccg
    10381 ctgtggatgg cgctgatctc gtgggtcgcg ttccgcgaca agcttcccgc cgtcggcatc
    10441 gccggcttac tcgcgggatt caccggcgtc gcgatcttgg ccaagccgag cggcgacttt
    10501 ccgctcgcgg ggtcgctgct gatcgtgctc gcggcgctga tctggtcgtc gggatcggtc
    10561 tacgcgcagc atgcgccgct gccgccccgc ccgttcctga cgaccgcgat ggagatgctc
    10621 accggcggcg cgtcgctgct gatcgtgggg ctgttgcgcg gcgaggccgc gggcgtagac
    10681 ctcgcggcgt tcagcggccg cagcatctcg gcactcgtgt acctgatctt cgtcggttcg
    10741 ctcgtcggct actcggcgta catctgggcg ctgcacaaca cccgcatctc gctggtgagc
    10801 acctacgcct acgtgaatcc ggtgatcgcg gtgatcctgg gtgcggccat cctgagcgag
    10861 ccgttcagcg cgctggatct gatcgcgggc tcgatcatcg tcggctcggt cgcgttgatc
    10921 gtggcggcca agaagccgac cccgccggcg agcccggccg atgctccccc accgccggac
    10981 gaggcgatcg aggcacagcc cgcacgaaat tgagaatcat tctcacctag tggtacgctt
    11041 gccccgtgca agcattcgga gccgcctccg cctcggtctg ggcatggtcg ctgctgtcgg
    11101 tcaccggcat cagcgccatc gcgctcgtcg gcggcttgac ctttctgctg cgcgagcagg
    11161 tgctgcggcc ggtcgtgctg ctgctggtgt cgttcgcggc cggcgcgctg ctcggcgacg
    11221 cgttcatcca tctgatcccc gaactcgcgg aagaggccgg attcgacctg agcctgtcgc
    11281 tcctgatcct ggccggcgtc ggggtgttct tcgtcatcga gaaggtcctg cactggcacc
    11341 acgcccacct cccgaccgag gaggtcatcc acccggtcgc gtggaccaat ctgatcgggg
    11401 acggcattca taacttcatc gacggcacct tggtcgccgg cgcgttcctg accgacacca
    11461 agctcgggat cggcaccgcg atcgcggtcg cgctccacga gatcccgcag gagctcggcg
    11521 acatgggcat cctggtccat gccggaatga gcaagaagcg ggcgctgctg ctcaaccttt
    11581 cgaccgcgct tgtttcgctc gccggcgttg cgctcgcgtt gctgctcgcc ggagcggtcg
    11641 atgatctgtc gcgcatcctg cttgccttga ccgcgggcgg cttcgtctac atcgcgggct
    11701 cggatctgat ccccgagctt caacgcgaga cgcgcatccc gaccgccatc ctccagctca
    11761 ccggcctcag cctcggcgtc gggctgatgg ccgcgcttac cttgctcgag tagcttcgcc
    11821 cgatccgcca cgctttgggg cggtaacctg agggctgccc cactcgttgg acctgttgag
    11881 gagccctcgc ctcaccagcg acgcgcgcgc cgaccgaagg ggaacgtctt atgagcaggc
    11941 tttaccggac cgcagcaggc gtcgcggctc tcatcgcgat cgggctcgcg ccgatcaccg
    12001 tgcccgcatc caaaccgccg aagcacgcgg cgatccgctt cgccccgaac gtcgtcgtcg
    12061 atcccgcgcg catgggcggc gagccgatca tggtcaccga ctccacgggc aacatctata
    12121 tctcttcgat catcggattc tcgaaccaca cttcgttcct gtggaagagc gaggacggcg
    12181 gcgagtcctt cgacctgctg cgcgtcccgc tgcccgccat ccagcggccc gacttcacgc
    12241 tcggcggcgg cgacacggcg ctgatcgtcg gtccgcccgc gcccggctac aaggacgata
    12301 cgttgatctt catcgacctg gaagggctcg cgtcgttcgg caccgcgacg accttcgacg
    12361 gcgggaatac gttcgtcaac gacaacgtct tcgcctccgg cgaccagccg ctgggcgacc
    12421 ggcagtgggg cggccactgg cgcgacaaga acggcgtgga ccactactac aacttcttca
    12481 acggcctgtt ttcgcccgac agcgggacca gcaccgcctc gtacgcaatc atcgagacca
    12541 ccgattacgg caagacgtgg aaggactgga agcgcaacgt cgtcaccacc ccgggccgct
    12601 cccggccggg ccccctattc gtggatccgg acaccggcga cctgatgctg acgtggacga
    12661 tcaaccgcag cggcaccggc ggcgcgggct tcacgctgtg caatcagctc aagcgatgcg
    12721 aggacacgat catcgccgag atgccgagct acgacaccaa caatacgttc gtgaccggcg
    12781 cccgcgaccg gcagggcaac ctgtacgtgg catggtcggc gatcccgcgc ggcgagctgg
    12841 atccggccga ggtcccgaca cgcatctacc tgtcgagctc gcgtaacaag ggccggacct
    12901 ggaccaagcc ggccgtggtg tcgggcaacc tgggcgccgc gtcgatgccc tcgatcgtgg
    12961 cgggcgacag cggccgcgtc tcggtcgtgt actacggcac gcccaagcag gggaacccca
    13021 acgacaacgc cggcccgtgg cacgtctaca tgagccagag cctcaacgcg ctcgacgcga
    13081 aaccttcgtt tacccgcgcg caggtcaccg accacaccaa ccacatcaac ccgatctgca
    13141 cgatgggact gggctgtacg ctcgacccga accgccgcga cgaccggaac cttatcgact
    13201 tcctgtacgc ggcgatcggc aagaacggcg agacgctggt cacctacggc gataccgcgc
    13261 atcagatcgg cccgaacccg ccggcgtcgt cgtcgatcac gatgttcgcc aagcagatcg
    13321 aaggcccgag cctgtatgcg agcgtcggcg agctgacgcc gcccgagccc gacgtgcttc
    13381 cctattcggg cggccagaac cgcgtctcca gctattgggc gtcggcggtg ccggacaact
    13441 ggcacgcgga cttggtcaag gacgttccgt tcccgcgtca cggtcccggc ggaccggggc
    13501 cgcagaatcc cagcctggac atcaccagcg cgtggctcga ggccgcaggc ccgaacacga
    13561 tgcggggaac gatgatgctg cgagacacgt tcagcgtgaa gatcccccag ccctacacga
    13621 acaacttcta catgttctgg tggtggagca agaacaaggt gcactacgcc gccgccgagg
    13681 tcggccccgc cgagtacggg atcagccaga ccgaggacgc gatctcggac tgctatgcgg
    13741 gcgagccgtc gtattcgaac ccggcgagcg cgcgatgggc gctctacgtc gcggctgccg
    13801 tgcccccgcc gaacgtgacg cgcatcgact gccacctgga cctgctgacc ggccgcctca
    13861 cgatggacat cccgctggac gcggtcggag ccggcatcgg cgacacgctc tactcggtga
    13921 cggcgtcgtc gtcggcgatt cacattccga acatcgcgtt caatgccgta gcgaatctgc
    13981 ccgacgaggc cgacgcggtc tcgccgttca cctacagggt cggctcgatc cgccagcaga
    14041 ccaccaagct cctgcggcct ccgccgcgca gcaagcgccg ctagacggct attcggcccg
    14101 gtccgagcga acacacgttc gttcgggtcc gggccgattt gcccagcctc ggctgcggcg
    14161 gctacaaatg ccccgtggcc aaagccattc gcggcccgcg tattccgcgc cgctccttcc
    14221 cccgaggacg catttttcgt gcgtcgctcg tgctcgcgct cgccctgtcc gccttcggat
    14281 tgatgccgca gaccgcacat gccgcctcga ccaagcagaa gctcaacgcg atggcgaacc
    14341 gcatcaaccg gctgcaatcc gcgcttgcga ccagcgagcg gcgtgccgcg tccatgaaca
    14401 agtcgatcgc ttcggcgagc tcgcggctca aggccgcgcg cgccggcatg accgcgctca
    14461 cgcaaggctt gtacatgagc gcgttcgacg aaacgacggc gctgctggcc gccgaatcgg
    14521 tcgccgagtt cggcgagcgg gtcgcgttcg ccgagcatct gcagtccggc cagaaggacg
    14581 tgctcgagcg cgtcgcgatc gcgcgcaccc agctggagcg ccagcgcaag gcgcttgccg
    14641 gcgtgctcgc gaagcagaaa gagctgctga agaacctcaa caaggagcag gccgcgctgg
    14701 cgagcgagtt ccgcaaggag cagcagcggc tggccgcgat cgagcgcgcg aaacgcgcca
    14761 agaaccgtca gcgagcctcc cgtgcggacg cgccctcgct gatctccggc acgggcccgt
    14821 tccgggtctg tccggtagat gagccacgcg cctactccaa cgactggggc gcgccgcgca
    14881 gcggcggccg gagccatgag ggaaccgaca tcctggcccc gcggggcacg ccgattcgcg
    14941 cgccgttcga cggcaccgtg aaagccaaca gctccggacg cggcggcaag caggtgcgcg
    15001 tctacggctc ggaaggctac gtgttcaacg cgcacctcga ccgacatgcg ggcgtatcgg
    15061 gcttcgtgaa agcaggcacc gtgatcgggt acgtgggcaa ctcgggcaac gccagcgggg
    15121 gcccgacgca caaccatttc gagtggcatc cgcgcaacgg cggcgcggtc aatccattcc
    15181 gctacttgaa caaggtctgc taaccccccg cgcgccggga ccccgccgca ccatcgcgta
    15241 cgctaacgct ccctgaaagg agccggcgtt gctgatcgtg gagttgggtc tggtgatcct
    15301 aggcctatcc gtcctggctc ggctcgcgct gcgcatcggg atcccgccga tcccgctcta
    15361 cgtcatcgcc ggcctcgcct tcggcgacgg cggcctgctg ccgctggtca ccacgcaaga
    15421 gttcatccgc atcggcgccg aaatcggtct gatcctgctg ctgttcatgc tcggtctcga
    15481 gtaccccgcc cgaaagctga tcggaacgct caagcacgcg ccacgcagcg gcggtctcaa
    15541 catcgcgctg aacttcacgc ccggcttcgt ggccggcctg ctgatgggat gggcgccgct
    15601 tcccgcggcg ttcctgggag gcgcgaccta cgtgtcctcg tcgggcgtgg cggcgcgcct
    15661 catcgaggat ctgcgcatcg gccaggaaga gcgaaccccg atcgtgtcca cgctgatcct
    15721 ggaagacctc gcgatggcga tgttccttcc cacgatcggc gcgttcctga tcggcggaag
    15781 cgcggccggc agcctgatga tcgcggtgag cgcggtcgcc atcgtgatct tgctcctgct
    15841 gttcgcgctg cgagccggca ccggcgtgag ccgtctggtc ttcgccaacg ccgacgagcc
    15901 gctgctcctg tcgatcctcg gcttcgggct gctcatcgcc ggcatcgccg aggtcgggca
    15961 ggtgtcggcg gcagtcgggg cgctcgtggc gggcatcgtc atctcgggtc cggccgcgca
    16021 gaacgtgcgg ccgctgctga gcccgatccg cgacctgttc gccgcgatct tcttcgcctt
    16081 cgtcggcttc agcatcgacc cgggccaaat ccccggcgcc ttgccggtcg cgttgctgct
    16141 cgccgggatc acgggagcga ccaagttcgt cgccggctgg aaggccgccg agtgggcggg
    16201 tgtgccgtgg ccgcgccgct cgatcgtggg tgcgacgctg ctggcgcgcg gcgagttctc
    16261 actggcgatc gccggtctcg ccgtcgcggc gcgcgtgcgt ccgaacccgg cgccgatcat
    16321 cgcgacgtac gtggtgatca tggcattgat cgggccggtc gtggcgcgca tcgtctcgat
    16381 ccgggtgcag cgcgccgaag cggactgagt cgcttagcgc gaggctgcgc cgcggatctg
    16441 cagcgatccc agcaacgtgc gtgacgattc ggcgatctgc gccaccgccc gctcgaacgc
    16501 ttccgcgttg tgcggcgcgg gcgcgcggaa tccactgatt ttgcgcacga actggagcgc
    16561 ggctgcctcg atctcgtcgt cggtcgcgac gacggcgccg tcacgaaggg tcttgatgct
    16621 cctgcacatg ccgcgattct agggctcgcg gccgcctacc gcgtcgccgc cgtttggtac
    16681 ggtcgcgtca tgggccgagg tgaacgcttt ctggcggcat ttcgccgagg gcccggccgt
    16741 gccaggggcc tcttccaaat aaacgaagca cgcgaccgga tggccagcgc aggctgcctg
    16801 acgtgcatgc gagaggaggc gccagggcgc ctcctctacg cgtacggcgg ctcgggctac
    16861 agagacgagt tcagagcacc gccgacattc gccatgcccg cacgacgcat gtactcggcc
    16921 tttccttccg gcagcaacgt gccggcggcg agcatcgcgt cgagtgaggc ctcgaactgg
    16981 gtcaggtagg cgccgggcgt gggatacaaa gccgccagcg tcgcggcatc aaacaccttc
    17041 gtcacgccgg tcagcggcgc ggcgccgccc gcggcgcagg gctcgccggt cgccggcacg
    17101 ccccccgcgt agtaggtcgc gatcggcgcg tccagcagcg ccgagcgcac cccgcccttc
    17161 atattgccgt gatcgtcgta gacccggccg ttcgcgtcgc gcgccgcgcg cggctgcggc
    17221 gccggcgcga tgccggtgcg gatccagtcg tcgagcgcaa ccagcgccgc gccccagatc
    17281 ggcccggcga agaagtggtt gtagatgccg cacgatccgg gcggaagctg gtagccccag
    17341 gcgcccgcgt cgtcggagtc gaagatgttc gcgctcccgt tcgagtgcgc ataggcgagc
    17401 atcgcccagc gatagttgtt gctgtagccg gtcgtgtgcg cgggcccgac cagttcccac
    17461 agacggaact ggccgctgtc gggcgcgacc tgcgaggttc cgatctcgga ctggctgttg
    17521 agccacagca ccggcgccac gtcgcgtcgc acgctcccgg cccccgacac ctgcggctgg
    17581 aagccgtcga agaccccgga gtcctcgtgc cagccgttga tgtaggtgtt gagacgtccg
    17641 gccgactgcg aggcgcccat cgcgatgcgg cgctgcacca gcggccgcag atcgccgagc
    17701 accgcgctgt ccagtgccgc ttcggccgcc tgttggtaga tgtcgaacga gtaggtgtcg
    17761 cccggatgcg acagcgactg gtagcggacc ggatcccact gcttgagcgc gatcgggctg
    17821 gcctcgatcg aaagaagctg cgccgcgacg ctgatgtagg catatccgcg gctgaagacg
    17881 gtctgccagt agttctgcca ggtcgcgtcg tggtcgtgcg ggatggtgac gttgttccac
    17941 tcgaagacca gcgtgccgtt gaagtcctgc gcgttggccg gacggcgaac caagaatcgc
    18001 gacgtgtacg cggcgctcgt accggtcttg ttcgggcccg aatatgccgt gccgccgtaa
    18061 aagaactcct cttcgaccca gccggccggc agcggcagca gcgactcgcc atagggacgg
    18121 ccggagcgca tcccgacgcc gtgcgggatc ggcccttcga ccaccggctg cgacaccgtg
    18181 gggctcggct ggacgcccga tgcccgcgcc gccccgctcc cgccgagcga agccagcatc
    18241 gcgcacgaaa gcgcagccgc aacaaaacga attctcatgc gagtcactcc tgtcagccga
    18301 tcttcggcaa accgtaggtg gcggcgcggc gcatcaggtc agcggcgcct tccggcacga
    18361 tgtttccggc cgccagcgcc gcgtccaccg cggcctgcag ccggctcagg tagtcgccgg
    18421 gcgtcggata cagcgccgcg agtttcgcgc cgtcgaacac ctcggtcatg ccggtgagcg
    18481 gaagccggcc cccggcctgg ctgcacggcg tcgggttcga tgcgccgccg ccgtagtagg
    18541 tcgcaatggg cacatcgatc accggggagc gcaccccgcc gagcaggttg ccgtgctcgt
    18601 cgtaggcgcg ggcgccgttc acccgcaacg cgcgaggctt cggcgccggc gcaacccccg
    18661 tgcgaaccca gttgtccagc gcgaccagcg ccgcggacgc gacgtagctc agcggatagt
    18721 aattgcggtt gttgcacgtg ccggcgatca gctgcgaccc ccagttcccg tgtgcttctt
    18781 cgtcgtaggg gtcgccgctg acgccgttcg agtgggagta cacgtacacg gcgttctcgt
    18841 acgacgaata ttcgttggtc gtgtgcgcgg ggcctgcgac ctcccacagg cggaacaggc
    18901 cgctgtcggc ggggacgtcg gtgttgccga tctcggacga ggagttgaac cacagcacgg
    18961 gcgccacgtc gcggcgtacg ctgcccgcgc ccgagacctg cggctggaag ccgtcgaaga
    19021 cgccgtggtg ctcctgccaa gtgttgatgt aggtgttgag gcgtccgccg gactgcgacg
    19081 cgccgagcgc gagccgccgc tcgaccttcg gccgcaacga tccgagaacc ttcggatcca
    19141 gcaccgcctc ggcggcctgc gagaagatgt cgaacgagta ggtgtcgccg ggatgcgaca
    19201 gcgtctcgta tcgcaccgga tcccattgct tgagcgcgag cgggcttgcc tcgatcgcca
    19261 gcagctgcgc ggcgaccgcg acgtagacgt agccgcgggt catgatcgtt cggtgcatcg
    19321 gcagccacga cacatcggtg tcgtccggga ccgtaacgtt ggtccaatcg atcagcacgg
    19381 tgccgttgaa ggcggcggga tcggccggcc ggcgaaccag gatgcgcgac gcgtacggcg
    19441 tggtgcgggc tcccgtcggg gtcgaggcgg cggtgccgcg gaagaagaac tcctcctcga
    19501 catacccgtt ggccagcgca accagcgagg tgcggtacgg ctttcccgtt cgaattccga
    19561 caccgtgagg aatcgggcct tccacgaccg gttgcgcgac gatggcgctg ggctgcaccg
    19621 gccacgacgg gctcgtggag ccaggggcgg ccactccgag cgtcaaggcc agagcgaggg
    19681 cggaggcgat caggcgaatg gctgggacgg ctgcggaggg catgatgtgt ctccttgggg
    19741 ggcgtccgag tagctcacac atatattccg tgaaggaaag cgctgcccct ttgaaggaaa
    19801 accgaatttt ggaacgaaag attgatcgaa atgatcaaag cagagcccga agctcccgtg
    19861 tggaccgttc gtgccccgaa aggctgggct cccgtggccg aggtctgccg tacggtcttc
    19921 gacgacatcg cggccgtcac cctgcgcatc aacggcatca tcgaacgctc gatccgcgac
    19981 ttcgcaccgt ccgagagccc gttcacgcac gaggaccggt tccgctcctc gcgagggatg
    20041 atcgccggct tcttgcaggc cgtcgccgag aaccgcgaca tccacgagcg cgacatcgcc
    20101 ttcgcccatc tggtgggaca gcagagcgcg ctcaaaggat tcccgctgcc gccgctgatc
    20161 gactcgctct acaccgccta ccgcgaggtg tgggccgagc tggtggccgc cgcaggccgt
    20221 gcgcccggca ccgcggacga gctgctgcga gaggccggcg cgacgatctg ggagcggatg
    20281 cacgcgctca ccaccgccgt caccgccggc taccaccgtc agatgccgcg gcccgaagcg
    20341 ctcgagatcc gcatcgccgg acagttcgtg aaggcgatcg cgaccgatcc ggcatccgag
    20401 gaagcgcggt cgctggccat cgacctcggc ttcaatgcgg aaggatcgtt tcgcgtcgcg
    20461 gccatcgccg agccggccgc cgcggtcgag gcgtcgcgcc ggatcgcgcg cgcggcacag
    20521 atggcgggag ccgtcgcgat cgccgcggct cggggcggcg cgtcggtgct cgtcgtgcaa
    20581 ggcatgcgcg aggacgacct cgcggcgctg atcgcccgag aggcatccga agcgccggcg
    20641 ggcatgagct tgaccgccga gtcgatcggc ggcatcggcg cggcgctgac gcaggccgag
    20701 tgcgcgctac gcctcgcggg gcacgggggc ggcctgcgcc gcttcgccga cgaatggttt
    20761 ctggccgcgc cgctgatcat gcgcgaggac atggagcggc tgctgcgccc cgcgatcgag
    20821 ctgtgccgca gcaagccgca tctcgcctcg gctgtgcggg cgttctcagc gcacgggttc
    20881 tcccttgcgg cggccgccgg agagctggcc gtgacccagt ccacggtccg ataccggctc
    20941 aagcgctggc acgcgatcac cgcatgggac ccgtggacgt tccacggttt ggcgaactcc
    21001 atcctggcct tcgagctggc cggcacaagc cgatagtccc ctcggcggcc agccgcggga
    21061 ggaacgcggc agccacggca tagcgacgcc ggctgcggct gctctgctcg gccccagcgt
    21121 gggccggccc aatgatctcc tggccacccc gcgctaagat gcattcgacg gccgccgttt
    21181 ccggcggccg tcgcgcatgt acggcatcgc gtcaagggat cctttcaccg gatctcaagg
    21241 gggcagacga aacgatggca ggccacgtgg tggagaaaca ggccgatccg aagacgctcg
    21301 ccgagtggaa gcatcactgg cgcgacgagt ccgacgcggc gttcctgtac ggcgtgctcg
    21361 ccgacgtcga gaccgacgag cgccgccgcg agttcttccg gcgcttcgtc gagctggaga
    21421 cccgccacgt cgaggcctgg gccgacctgc tgcgccgcaa cggcgtcgaa gtcggcaagc
    21481 tcgccaagcc caccgcgcgc acgcgcctga ccgcgtggct cgcccgccgc ttcggccccg
    21541 ccttcctgct caacatgctg caccgccagg agggccagga ggtccgcacg tatctgaagc
    21601 tgcacgagtc ctcacccgcg ggccgcgcga aggacacggc gctcacgctc gcgcaagagt
    21661 cggccggcca cgccgaggac ctgcgcaaga tgtcgtcggg cgacggcgag tcgtggcacc
    21721 gcaccggtgc gggcggcatg ctgcgcaacg tcgtttacgg cttcaacgac gggctgaccg
    21781 cgaacttcgg gctggtcgcc ggcgtcgtgg gcgcgtcggc gtcaacgtcg gatcaccaca
    21841 acgttctggt cgcgggcatc gccggcgtcg tcgccggggc gatgtcgatg gcctcctcgg
    21901 gctacctggc cgccaagagc gagcaagagg tacacgatca cgagatcgcg ctggagcgcc
    21961 aagagatcga gctgatgccc gaagcggagg aggaagaact cgcgctgatc tacgaagcgc
    22021 gcggcatcga gcccgaacgc gcgcgcaagc tcgccgccga cgtgatgcgc gatccggagc
    22081 gcgcgctgga agagaaggtg cgcgaggagc tcggcatcgg cgagccgcac accacgccgg
    22141 tcaaggaagg cgcgctcacc ggcgtcgcta ccgcgatcgg ctcgttcctg ccggtgtttc
    22201 cgttcatggt cttcgacggc gtgactgcga tctgggtttc gttcgtggtc gcgatggcct
    22261 cgcacttcgg cgtcggcggc atgcgcagcg tcttcaccgg ccgcggcctg atccgtagcg
    22321 gcatcgacat gttcatcgtc ggcctcggtg tcgccgtcgc cggctacttc gtcggcgaat
    22381 ggatcaccga gctgctgtag cgcgcttgcg gaagtaggtc cagctggttt cgttcggctg
    22441 ggtgaactcg cgcggggctt cggcgaactg cggaccgccg gcttcgatct cgtcgcgtac
    22501 gagcggcggc agatccgtgt agccgccggc gagcttggcg ccccggccgt gatagaccag
    22561 cattccgggc cgatccccca tccacatcca cggcagccac ggacccagtc gcgtccacga
    22621 cacctggctc ggcacgtccg ggttggattc gtcgtccacg tggcttcgct tgcaaaagaa
    22681 ctggaacaac tcggccgcct gatacaggtc cgactgcgaa ttctcgggga attccgcgcg
    22741 cggcagcggc gacggatagg caaggaacac gtccacgttg aagaataggt cgtcgccggc
    22801 ctggctgacg ggaacgttcc acggcccgta gtggccttgc aggcgcatct gctggttaac
    22861 cgggtcgttc cagatgtgca gcacatcgac gctctcttgg ttgagcgggt tgtcgaatgt
    22921 gcgcaggatc tcgccggtgc gaaggtcggt gtagaagacc gcctcgcggg tcagcaagtc
    22981 gtagccgccg tcggcctcga ccacgcgcga gacgttatag ccctcgaagc cgaagagcgg
    23041 ccggccgcgc tcgccgggca gccatgcgtg ggtcgtgccc ttgaaccagg tgacgctgtc
    23101 gctgccgtcc agcgacgagc gcacgcgcag gaacgcgtcc atgttctgct tcggatcggt
    23161 caggtccacc atcacacgac ccgcgtctcg tcgatgatcg aacgctggaa cagcacgccg
    23221 tccttgaacc agtgccacgt gcgggagcgg ttctgcgcgg cgtcgtcgag cgagatcatc
    23281 tcgtagaggt agatgccggg cgactgcacg tagacccagt gcagcacgat cgtgcggtcg
    23341 tccacctccc acgcgtgccc gttgatgcgg tcggtgtcga accagatctt gcggtccttg
    23401 tacgccgcgg gaaactcgat cgtttcggtc cgtccgtcgt cccaggtgta gtcgttgacc
    23461 tggtagtagg gcgggtcccc gctttcgggg aacgagcacg tgagatgcga gcggtgacga
    23521 tcggtgacgg tcccgtcgcg cgcgatgacg gtgtaggtcc cctcccactc gccttcgtgc
    23581 cgcgcgagca gcggcatctc gtccttgatt cccatgaaga ccggcctccc cgagcgctac
    23641 gcgtgccggg atcttgatcc attccgggga accgagtcaa cgcccggtca gcgaagatgc
    23701 gggtaccacc acgggtcggg ccgttgcgcg tcgctgatca ggaaggcctg ccggcggccg
    23761 aagcgtcgca gcgctgccgg gcccatccgc gacccgccca tgccgctgaa gttgaacgag
    23821 ctcttttcgc cgtcgtagac gagcgcggtc agcgcggcgt cgttgatcga aatcccgccc
    23881 gcgtcgatgc ggcgtgcgac gccgagcgcc tcatcggcgt caccgaacac cgcgccgctg
    23941 aggccgtacc gcgtgtcgtt ggcaagccgg actgcctcgt cggcgtcggt gaaggacatc
    24001 accgggatga ccggcccgaa cgtctcttcg gtcatgacct tcatctcgtg cgtcacgccg
    24061 gtgagcacgg tcggcttcag gtacgagccg ccgccgtgct cttcgatctc gcccccggtg
    24121 tgcgcggtcg cgccgcgcgc gagcgcgtcc tgcaggtgcg cggcgatcac ctcgatctgc
    24181 ttcgcggcga tgatcggccc gatgtcgccg gcgtcgggct cgggaacggc caggcgcacg
    24241 ctcttggcct tgtccaccag ccgatctacg aagtcgtcga agacggactc gtgcacgtaa
    24301 acgcgctcga tcgactggca cgactgacct gcgttcgcgg tcgagcccca caggatcgcg
    24361 ctcgatgccc ggtccaggtc cgccgacggc agcacgatgg ccggatcctt gccgccgagc
    24421 tcgaggaacg cggggatgaa ccgtttcgcg gcccgggcgg cgatcttcgt gcctgtggcg
    24481 acgctgccgg tgaagcaaac gacatcgctt cggtccacga tcgcctctcc ggccgagccg
    24541 tcgccggcga cgatcgcgac gacgttctcc atggccggga ccgtggcgac ggtggcggcg
    24601 agcggctcga tgaagcgcgg cgcgatctcg ctcggcttga tcaccaccgc gcagcccgcg
    24661 acgagcgcgg gaaccgcgtc gatcagggcg agcagcagcg ggaagttcca cgggctgatc
    24721 acgcccgcga ccggatacgg ccgcatgccc tgctcgaggt cgatacccgg gattgatgcg
    24781 ggccggcgct cggtcccggc gagcaggtcg ggcgcgaggg agcaccaacg ctcgatcatg
    24841 cgcagcgccg catcgacttc catctcggat tcgacgcggc ggccggtatc ggccatgagc
    24901 gccgcggtga tctcgccccg cgccatgccg atcgcgtcac gccagccgtt caatgcggcg
    24961 gtacgaacgt cgagcccgcc cgcttcccac acggcctgcc cgtcgcggag ccgcagggca
    25021 agctcgtcga gatcgtcggt ggacggtggc tcgaacgagt agtcgcttcg gccggtccgc
    25081 gggttgcgta cgtcgatcgc catgatgccg tcagatgatt ccttccccat cgagccgctc
    25141 gataacggct ttctgggcat cgaggaacca accgcggccg ggctccttgc cgtcgccgag
    25201 cccgttgggc gcgaagtgct tcgcatcggc gtacgacgag gcgtagagat cgaaccggct
    25261 gcgggcgagc atgttgccca tcccgggacg gcgccgcgcg cgacgcagcg acgcaagctc
    25321 gacgggcgcg gcggcgacca tcgactcccc cgggcccgcc tcgatgagca cgcggccttc
    25381 gtaatcgacg atctgcgatt tgccgtcggt ggagtcgccg gggatcggcg agccttcaat
    25441 gccaccggtg ttcgcgctca cgacgaaggc catgttctcg atcgctcgcg cgcgtttggc
    25501 cacgtccttg atcgtcgcct gcgggctcga cgcctcggac gtggagtggc agaagacctc
    25561 ggcgccgcgc atcgccagac agcgcgccac ctcggggaac aggatctcgt cggaggcgat
    25621 gcacgcgagc gttccgatct ccgtgcgaag gaccgggaac agcgaatcgg cgccgtagac
    25681 ctcaaggtac cggtcgagca cgtcgtgcgg cgtcggggtc atgaacgagt tcaaccgccg
    25741 gtagcgcaac gcgagcgcac ccgacgggtc gaagatcagg cacgattgga agtacagatc
    25801 cggaaacgcc gggtcccgct cgtagaggtt cgcggccagg tacagggtat tcgcctgcgc
    25861 gatcgcgccg agcgcctcct cctcggcgcc gtccttgtcg accgcaacgc attcgcgcca
    25921 ctgatcgaac gcctcgccgg ccggatggcc cgtcagcacg tactcgggaa gcacgaccag
    25981 ccgggtgtcg ggaccggtga acgccacgct cgccgcgacc tgcttgccga tccgggcgat
    26041 ggcggcgcgg atgatgatcc ggccctgcgc cgggtcgtcc acccccgaga gggccctgca
    26101 gcgtgcctgc agcgcaagcg cgcgatacga ctcttccacc cggggccccc ttgtcgatgc
    26161 gacgatggtc catgatatga aacagggtgg gcgccgggaa gggggatcgc gtcatcgaca
    26221 caccggtgga catcagggtt cgtgaccgcg tcgccttcct gacgctgtcc cgtcccgagg
    26281 cgcgcaacgc gatcgacgag gcgatgctcg aagcactgcc gcccgcgttg cggcgcgtcc
    26341 gagacgaccc cgacgtgaag gcgctcgtga tcaccggtgc cggtgacgcc ttttgcatcg
    26401 gcctcgacat cgacctgctc gagcgtgcat tcgacgaccc cgagtacttc cgcgatgtcc
    26461 tcgagcgctt caagcggatc ctcggcgaca tcgaggcgct gcccgttccc gtcgtggcgg
    26521 ccgccaacgg cctgacccgc gcgggcgggt tcgagttgat gctgtcatgc gacctggccg
    26581 tggtcgcggc cgaggcgcgc atcgccgatc atcacctcgc cttcgggatc gtgccgggcg
    26641 gcggctcgac gcagcggctg cctcgcaaga tcggcgagca gcgggcccgc gagatcatct
    26701 tcaccgggcg ctggctgacc ggggacgagg ccgcgcacta cggccttgca ctgcacgcgg
    26761 ctccgctgac gcggctgggg gatgcggtcg aggacgtggt cgcgccgctc cgccaccggt
    26821 cgcgaaccgc gctggcggcc acgaaagcgg cgatgaacga aggtgcatcg ctgccgctgg
    26881 tgcaagcctt gggggtcgaa accgacgtct ttatgaggta cctgacgaac gacccgcacg
    26941 ccgccgaagg gttccgcgcc tatcgggaag gacgtgatcc gcaatggccc tagagatctc
    27001 cagcttcgag gcgttcgcgc cgggcacgtc gttcactacg ccgtctctgc acatcgacga
    27061 aagcgatgcg gccgaactga tccgcatcgg cggctacgtg catccgctgt tcaccgaccc
    27121 gacctatgcg gccgatagcg ttttcggccg ctcaccgctt ccgggcgagg cggtgctgca
    27181 tgcgatgggc ggactcgccg aacgctcggg ccggttcgac gagtccgtcg tcgcgttgct
    27241 gggcttcgac gccgtgcgct tccacgcgcc cgcctttccc ggcgacacgc tgcacatgag
    27301 cgtcgaggtg atccggcgcg aggcgcggcc caacggcaag cgcggagaag tcgtgatggc
    27361 gtggcgttgc cgcaacgagc gcggcgaact gctctgcgat gcgacggcgc gaatgctgtt
    27421 tcgtctgcct cacgcgtaga gcggcgcgga gatgtcgaac cattcgacgt cggagcacag
    27481 cgattgccat tcggcgaaca gcgcttgcat ctcggcgggc atctctttct tctcgcccgc
    27541 gcgggcttcg gcttcggtgg tgaaccagtc cacgatgtgg atgcggttgc cgggaagcca
    27601 gacggtcatg tggcctataa ggtcgggccg caggccggcg aactgatcga acttctcttc
    27661 gatcgcttcg taggccttcc ggtctttgac cgtcgcgcgc atcatctgca cgaagcctgc
    27721 gtcgcctgat ccttccttcg gcatgtccac gtcttcggac tccaggaact ctgcggggcc
    27781 ggccagcatc ttttcgagct ggttccaccg ggccgtttgc tcttcgctgg ccgagttctt
    27841 catcgccagc tcgcggtttt cgaaccgtgc ggcgatcagc agctcgccct tggtggtgat
    27901 gccgccggtc gcacccagaa atccttcggc cgtcgcgtcg cgctgctctt gccatgcgct
    27961 gttgatgccg tccgcgtccg cgaccttcgc gcgaatgatc tgtacgaaca tctcgcccct
    28021 ccttcgtcga tccctcgggt cgatcctccg cccgcgcggc gtgggaagtc aactcgctct
    28081 gggggtgacc tctttgtcga tccgggcacc gctgcggtca cgttcgatct cgaggtcgaa
    28141 caagacctgc aggttgatcc aaaagcggtc ggacgtgccg aaatagcgcg ccaggcgaag
    28201 cgccgtgtcg gccgagatcc ctcgtcggat gcgcgcccct cacgccatcg aaagcagatc
    28261 cgccattgat cgttgatccg gatgctgtac tgacccgatc ggtcgccctt gagccgctcc
    28321 aaacgatttc ccggcggaac cttgaggtcc tccaacgtgt tggccgcgtc cagcatccgg
    28381 agccggcgca aagccggccg ctgcacgtcg gacccgaagc gcttgacacg ctcgcgccgg
    28441 tacaggcgtt cggtgtcgcg atccttgaag ctttcgatca tgacgcatag gaacgacctc
    28501 cgtgaatacc gtcaagcgtt aggctggcga cctttgaccg gctccgctcg ctccgggtat
    28561 caataggcgg aacgtcgttc cgctaggaga gacaatgcag ctgtcgtacg ggccgtgggg
    28621 agcgtcgctc gacgaagtcg tcgcggcctc gaagaaggcg gaagacgcgg gcttttcacg
    28681 ggtctggttc gccgagctcc atcgcagcgc gttcgttccc gccgcggccg tggcaaccgc
    28741 gaccacgcgc atcggcgtgg gcaccgggat cgcgctcgcg ttcgtgcgct ccccgatgac
    28801 cacggcgctg gaggcgatgg acaccgacga gctggccggc ggccggttcg tgctcgggat
    28861 cggctccggc gtgcagcggc tcaacgagag ctggcataac gtgcgcttcg gcaaagccgt
    28921 cggccacacc cgcgagaccg tcgcgatcat ccgccggttc atcgccgaat cgcaccgcgg
    28981 cggatcgatc gaggtcgacg gcgaatacga acggatgagg ctgcgcggct atcagcggcc
    29041 gttcccgccg cagcgcgagc agatcccggt ctatctcgcg gcggtgggcg acgcgatgac
    29101 gcggctcgcc ggcgagatcg gcgacggctg gctcggccac gagctgggat cgccgcggta
    29161 tctggcgtcg gcgatcctgc cgaaccttca agcgggcctg tcgcgcgcgg gacgcgaccg
    29221 gcggtcgctg gacgtcgtcg cctcggcctg ctgcgcgatc gacggcgacg ggcggctcgc
    29281 caaacgctgg atggccggcc tggtcgcctt ctacgcgacc gtgcgaacct atggcgagtt
    29341 cttcgctttc cacggctttg ccgccgaagc ggccgcggtg caggagcggt ttcgggccgg
    29401 cgacgaggcc ggcatggtcg aggcggtgcc cgatgagatg gtggacgcgt tcgcgctcgc
    29461 cggcacgccc gacgaggtgc gcgccaagct gaaggaatac gacgggatcg ccgacggcat
    29521 caagctgtcg ccccccacgc atctggttcc ggccgaggtc acgcgccact gccaagccac
    29581 gatcctggag cagctcgccc catgaacgcg atcaagcccg cgtcgtttcc ctggttcgag
    29641 tacgcgcgct acacgttctc gctcggcctc gaacacgcag gccgcgtgtg gctcagcggc
    29701 cacagtgcct ccgagtacga cgcgcgcgag gaacgcatcg tcgtcaaagg cggcatggag
    29761 cagcagaccc gcaccgccta cgccaagatc agcgcgatcc tggaggcggc cggcctgacg
    29821 ctgggcgacg cgacccgcgt cgtcgagtac gtgaccgcga aaggcatgga ccactacgcc
    29881 gaagcgcgcg cggtccgtga agagctgttc ggatccgcgc agccggcggt gtgcaccgtc
    29941 gtggtcaaca gcctgctgcg tcccgccgcg ttcatcgaga tcgaagtgac cgccggcggc
    30001 ggcgaggacg acggcatcgt gtacctgccc tcgatcgccg cacccgaggc gggcgaagat
    30061 ctcgtcgcgc agaccgaggc catcttcgac aaggccgccg cgctgctcgc cgatgccggt
    30121 ctgggaatgg actcgatcgt caagaccgtg gactacacca cgccgcgaac gctcgccggc
    30181 tacaagcgaa ccggccgcgt tcgcaaggac cggctcgggc ccgtgtatcc cgcggcggcg
    30241 ggcatcctga tgtcgcggct gcagacgccc ggcacgctcg tgtcgatgga catcacggcg
    30301 tcacggcatc agtcggagac cgtcaatccg ggttgggaac gctacgcgaa gctcacctac
    30361 tcgcccgcgg tgaaggccgg gtcgatgctg ttcatgagcg gccaggccgc gctcgatccc
    30421 gcgaccgagc aggccgtgca caccggcgat gtcgccgcgc aagccgagta cacgtatcag
    30481 aacatcatcg aggtcctgaa ggcagccgga gccggccccg agaacttggt caaaaccatc
    30541 gagtacgtca cgcctgccgg gctcaagcgc taccgcgagg tcgccgccgt ccgcgaacag
    30601 ctgctgaaac agccctaccc cgccagcacc ggctgcatct gcgagaccct cctccgcccc
    30661 gaattcgaaa tcgaaatcga cccgctcgcc atcctgtaac ggcgcacgcg aatccgttca
    30721 ccggacgctc tttggagaag tggccgtcag ccgaggatca tcacggttgc ggtcatcgat
    30781 gccttgatgg tgccgtcttc tacgagcggc tcggcgtggc gggcggctac ctcgcgcagc
    30841 cggatcgccg tagcttcgtc gagctccgca agctcggccg cgataccgga ggcaaccagc
    30901 gtgtcgaacg ctccggggaa cccttcctcg atgatggcgg gcagcgtcac ggtccgggcg
    30961 gtgaccgatt cgaatcccgc ggcttcggca gccgacgcgg ccgcgtcgcc gtagaacgaa
    31021 aacggacccg tgtagcgcgt ggccatctcc tggccgagca cctcacggat cgcctcgtag
    31081 agaaaatgga acgcttgctc ggacacgtgg gtccatgtcg cgacgcctgc gcggccgccg
    31141 ggcctgagcg ctcgccgcat ctcggccagc gacgcgaccg gatcgggaaa gaactgcagc
    31201 ccttgctggc acgtgatcac gttgaacgac gcgtcggaaa ccggcaacgg ggcggcgggc
    31261 gcttccatcc actcgatcgg agcaagaccg gacggcagat ccttctcgcg gcccagcgcg
    31321 atcatcgccg gcgagatgtc ggtcgcaacg acccggccgg cgggccccac cgcctgagcg
    31381 agcaagaccg caaccgtgcc cggcccgcag gccacgtcga gggccgcttc gccgggatcg
    31441 ggatcgagcc ggccgaccag atcgcgcgcc cacggctcga acaagcgagc gatcacgcga
    31501 tcgtagacct gcatcgcatc ccgtgacgcg ccggcgaacg cggcgctcca tgtgcttcct
    31561 tctacgctca tcgccacccc cttccccccg ggacgtctcg cacaggctag ccgaccgggc
    31621 cggaagattc tttctaacga aagtgtcgag gcgcacggcg gtccgaaacg cgaagcaggc
    31681 ggcgatcaac tccgcgaacg cgaagagatc ggccgcctgg ccgcgttgct accgcctgga
    31741 gaagccccat cctcctccga ggatgagcaa caccactagc acgatcagaa cggtccgcac
    31801 gatgggtctc cttccgtcac gatgaacgtt ccgctccttg ccctgttcga aaccacgcga
    31861 ctcgcggaag ggaactacgg accgcgcgtg agcgagtctc ccccggctgc atgatcgcct
    31921 ccgcgcggcc cgctgctagg ttcggcatac cgtgacggcc gcggacattt ccgccgcgtt
    31981 ggtggcgcta accatcgtca tcgggtcggc gcacgcgata ggtcaggtga tggagcgcct
    32041 gcgccagccg cggctgatcg gcgagatcct ggccggagtg ctgctcggac cgttcgtctt
    32101 cggccgggtc gcacccgcgg tgacccgcac cgtgttcggc ggcgacgcgg ccgatgacac
    32161 gacacgcatc gtcatcgagt tcgtgtcctg gctggggctg ctgctgctga tgtttctgtc
    32221 cggcagcgag gcccgccgcg tgctcgcacg cgagaaccgg cgcgcaaccg catggctatt
    32281 cggtctgggc acgccgcttc cattcgtggc ggcgctcatc gtgggatcat ggatctcgct
    32341 cgacgggctc gccgggccgt ccggcagccg cgcatccgtg ctgttgatcc ttgccgccgc
    32401 cgcgacagtg atgtcgatcc cggtgatctc gcgcatcttc cacgacctcg ggatcctggg
    32461 aacgcgcttc gcgagcctgg tgctcagcgt cgcgatcgcc gaggacatcg tgctgtgggc
    32521 ggtgctggcg atcgcgaccg cgctggcggc atccggcgcg ggcgcggccg gcggcatcgc
    32581 gacccacgtc gcggcgaccg tcgcgtacct gttcgccggc gttgcggtcg ccccggcgct
    32641 ggtcaaacga ttgcacgacg cgcgatggaa ctcgctggcg accgcatcac ccgtccggta
    32701 cgcggtgctg ctgctgctcg tctacgggag cctggcatcg ctgatgaacg tgaacctggt
    32761 gttcgccgcg ttcctggccg gcttcggact ggtcggcgga cgagaaggcg catcgcggga
    32821 acgcttcgcc gtgccgctgg aggcgatcac cagcgtatcc acggccgtgt tcgtgcccgt
    32881 ctacttcgtg gtggtcggct cgcagctgca gctcggacat cagttcgcgc cggtcatgct
    32941 ggcggtcttt ctgatcggct cgaccatcgt gcgggcggcc tcggtcgccg gcgcggcgaa
    33001 gctggccggg ttcgcgccac tggaccggat caatctggcg gtgacctgca acgcgcgcgg
    33061 gggcccgggc atcgtgctgg ccagcatcgc gttcgaggcc ggcatcatca acggccgctt
    33121 cttcaccacg ctggtcctga ccgcgatcct gacgtcacag ttcgcgggag cctggctcgc
    33181 gtgggttctg cgccgcggac ggccgctgct gtcggacgac catgccggcc cggaacggca
    33241 cgaagcatcc gcgtcgccgc tctccgggca ggggcgcggc ccgcagcgga cggccgtcga
    33301 gcgccgacga cggagacagc gctgaaccca cgtttcccgg cgccgtcggc agcctccgga
    33361 tgtgacggaa tcggcatccg tcgtcgaaga tgacgggcga gccgacgggg aggagacgcc
    33421 gtgtccgagg atcttgctgc cgcgtactgg caggagttcg cggagcgcaa cgcgctgcga
    33481 accggcacca aagagcagaa gctcgaatac caaacgcgcg tcttcccggt cgaagacggc
    33541 atcgcgacac gcatcgacga cgatgaccct gccctgctgg ccgatatgat ctcgatcgcc
    33601 gaagcggcgc ccgacgacga atcgcgcggc atgttcggcg ccggcatcct gtgggagtac
    33661 ctgtacaaca cctcggacaa gaacgccgcc gcgatcgccg acgccgccaa gaatcatggc
    33721 gcgttcctgg acgccctgcg cgccgcgttc gccggctggg acggcctcgg ctcgtccatg
    33781 aagcagaacc tcaaaccgct cctcgacgga tgacctcgcc gtggatctgc tgacgcccgt
    33841 cggcgggatc gccgccgtcc tggtcggggc tgcgctggtc gcgggcctag tcgagcgcgg
    33901 gcccatctcg ttcccgatca tcttcctggg cgccggcctg atcctgggtg acggcgtgac
    33961 cggcgccgtt cacatcgatc tgcacgcccc cattctcgag atcgtcgcct tcgtcaccct
    34021 ggcgctggtg ctgttcttgg acgcggtccg gctggaaacg aaagagatgc gccgcgactg
    34081 gctgatcccg gcgctgacgc tgggccccgg cacgatctcg gtcatcgcct tggtcgcgac
    34141 cgccggcgtg gtgctgctgg acctgagctg gacggtcgcg ttcatcgccg gcgcggcact
    34201 ggcatcgacc gacgcggtcg tgctgcgcga cgtcctgcga gacaagcgca tcccgggacc
    34261 ggtacgccgt tcgctgagca tcgaagccgg aacgaacgac gtgatcgtcc tgccacccgt
    34321 gctgattctg gccacggtcg ccgcggccaa ggctgcgacg gcagcggact gggcctggtt
    34381 cggcgtgcag ctgttcctaa tcggcccggc aatcggcgct gcggtgggcg cgggcggcgc
    34441 gtgggcgatg tcgcgagtgg atcgccgcac cccggtccgg accgaatacc aagccctcta
    34501 tggcgtcggt ctggtactga cggcgttcac gctcggcgag tggctcggcg ccgacggctt
    34561 cctggcgtcc ttcgcggcgg gtgccgcggt cgcgctgacc aacaacgatc tgtgcgagtg
    34621 cttcctggag ttcggtgaag tcgtcgccga gatggcgatg ctgctgacct tcgtgctctt
    34681 cggcgcggtc ctgtcaccga tgctcggcga gaccgtcggt ccccccgtgc tcatcttcgc
    34741 cgcgtttacg atcttcgtcg cgcggccgct gccgctactg tcggtgctgt cgatccggca
    34801 caccgagctg agccatcccg cgcgcgcgtt catcgcctgg ttcggcccgc gcgggctcaa
    34861 ctcgctgctg ctcgccctga tcgccgtggg cgccggcgtt cccaacggcg agcggctgtt
    34921 cgccatcatc ggcgtcgtgg tggtcacgtc cgtggtcctg cacggcgtct ccgccacgcc
    34981 tctgtcggcc tggtacggcc gcagcgtcga gcgcacgacc cagcccgaag aacggcatgc
    35041 gaccgcaacc ggcctgttta tcgccgatcc cgcatcgcag cagcggatct cggtggacgc
    35101 gctggcgaca ctgctggcgt ccgcggatcc gccgatcgtg ctcgacgttc gttcccggtc
    35161 cgcattcgag cagtcggacg cgcagattcc cggaagcatc cggctgcgtc tgaacgagat
    35221 cgacgcgtgg gccgagcatc agaccaagga ccggctggtc gttctgtact gcacctgaac
    35281 gaacgaagcc tcgagcgccc ggggggcgct catcctcgcc aagcacgaca ttcccgcagc
    35341 cgcgctggtc ggcggctgga atgcgtgggc cgctacgcag ccggtcgagc cgatcgtgca
    35401 cgagcccgcc ggctgatcgt gatctgcggt gccgtcgtcg cgcttcggcg ctacgacagc
    35461 accgacagaa acgttcggtt cggggaaccc aatcctgtat tggtgtcgta gccgcggcgg
    35521 ggcgggttcg acaggaactc ttggtaggtt ttgagtacca ccgtctggcc ctgcgacgcg
    35581 tccgtcgcgt tgacccactg ccggcgcacc tcggccttga cgcccggaat ctcgacgatg
    35641 tcgcggaacg ccgccggctt ggtgcgcgac agcgcgtaca gcgccggatt cacgaacccc
    35701 gccgccttgc cgctgcgctg gttgaccagc gtcagcatcg cgccgaacag ggccgtggac
    35761 gcggtatcgg cgttgatacg gcgctccgcg tacttgacgg tgccgtcggg ataggtctgc
    35821 gtgtagccga tcatcaggcc ggtctcagca tcgccgttga ccgcgatgtc gggcacggtc
    35881 cggccgtgcg ttccgtcgct gcgctgcgac agcagatacg gaacagagcg ccgctgatag
    35941 tccggctgcc cgtagatcgc gctccggccg ccgccgcttc cgcggaacga gcccgccgac
    36001 ggatcggacc atgcgccgtc gcgcaggacg cgatgcgtct tggcccagcc catctcgccg
    36061 aggtatgcat cgccgcggcc gaggatgagg ctggtcgcgc cgacggcggt cacgagcggg
    36121 ttgtgcgccg gaaacgacac cgagggatgc gccgggatca ccggaacgtt cggcacgacc
    36181 acatccggct cgcccgagaa cacctcgagc tcgccggtct cgtaggcgtt gaagtacgag
    36241 ttgtcaccca gatcacccga tgcgaacagc agcgagatgc cggtcgcggc cgcctgctcg
    36301 gcggcgcggt cgatgggcgc ggtgtcgccg ggcgtgacga gcgcctcgct caccgcgtac
    36361 cagccgttga cgatcacgtt tccatagtgc tgatccaccg cgagcagcgt cgcgttcggc
    36421 aggtccatcg aggacgtgga gccgatgtaa cgcaggtcgg cacccggcgc gatcgcgcgc
    36481 atggtctcga aaagcatcgc cgcctggacc gcccacaccg ccggccgcag gaacgatccc
    36541 gtcggatcgt tcggcgtctc gtaggcgccc ggatacgaga attggcggaa ctgctcgggc
    36601 cgcagcgccg ggatccggtt ccgcttcgac cacgtgtcga tgtccgacgg caggctccgg
    36661 atcgcgtgcg agccgatcat cacgatcgtc tgcccgcgtc cgtcgacctt acgcgcgtgc
    36721 agcgtatcgg cgccgtaggc cttgcgaagg cccgcgaccg acacgctgca tcgaaccgcg
    36781 ggattggtgc gcccgtacgc gggcggtgcg ccgcgatcga gcgtggcgcc gtcgtaggcg
    36841 gagcaaggcg cggcgtaggc cacgtaggac ggcggacgcg catccgccgg gttggcgggc
    36901 gtctcgggca cgacgggcgg gtcctccacg cggctcgggc gaaacagcgc cgaagcctcg
    36961 tcgagtcctc ggatggtcag gccgaggccg gcgagatgcg cgggaagcga cggcgcgcgg
    37021 tcgggtgcgc gcagcgtcat gccgccgaca cggtaggtcg acagcgtcac gccgaagacg
    37081 cgctcgatct gcgcggccgt gccgcgcgcc ggcaccacga cgcgattcag cggcatccgt
    37141 ccgaccacca gcccctgcga gcgcagccac gaaaccaccg catcgacggt cgcggacgcg
    37201 ggcgcgaacc gcgcgcggaa cttctcgctc gtcagatacg cccggtacga cggcgaggcg
    37261 ggatccgaca cggcgcggac cagccgcgac accgcggcgg gatcgcgcca cggcaagatc
    37321 agctcgacgc gaacctcgcg ggccgggtca acgcgcgcga cacgcgcgga ggccaccgcc
    37381 caggacggca cgagcgacgg aaacgatacg ggggcggccg cctgcgccgg gagcagcccg
    37441 gccgccagga tcgacgccgc gagaagcagc gggaatcgtc tcatacccgg gacgattcga
    37501 cgccgctcgc cctgctcctt cccgctacag gcgcagcggc ttgatcggtg agccgatcag
    37561 ggacgggtcg gaccgccagt cgacgccggg aacgtcgatg tagagctcga tctcgttgcc
    37621 gtcggggtcg gcgatgtaga ggctgtgggt gacggtgtgg tcgctcgcgc cctgaatcgg
    37681 gacgtcgttg tcctggagct tcttgacgac ctcgcgcagc tcgtcgtcgc tgtcgcccac
    37741 cttcaggccg aagtggtaca tgcccacgcg ccggccttcg gggagcggcg ccgcgttctg
    37801 gccgacctcg aggagcagca gctcgtggtg ggtgcggccg ttgtcggccg agaaggccgc
    37861 gaccgggatc cggctcggct cgttcggatc gggcaggatc tgccgccacc ccagcacgtc
    37921 gcgatagaac cgtaccgacc gctcgatgtc ccgcacgtat agcaccacat gtccgagctc
    37981 tttgacctgc atgcctcatc gcctcctgcg gaagcaaacg atagtatggt cgtcaactat
    38041 tcccacggcc tgcatgaagg cgtagcagat ggtcgggccg acgaaccgaa atcctcgctt
    38101 ggccagcgcc ttgctcatcg cgatgctcac ctcggtcttg gccggcagct cgtcgagtct
    38161 ttcgaagcgg tttacgaccg gcttgccctt cgtgaacgac cacacgaaat cgctcagcgg
    38221 ctcctcgagc gccaggatcg cgtttgcgtt cgagatcgtg gactcgatct tcgaacggtt
    38281 ccgcacgatc cccggattct tcatcagccg atcgacgtcg cgcttgccga agcgcgcgac
    38341 ggcgacggca tcaaatcccg cgaacgccgc gcggtagccc tgccgcttgt tgaggatcgt
    38401 gttccacgac aggcccgcct gcgcaccttc cagcgtgagc atctcgaaca ggtgcacgtc
    38461 gtcgcgcgac ggcacgcccc attcgtcgtc gtggtaggcg accatcaact cgctgccgcc
    38521 ctccgcccac tcacatcgct tcatcgaggc gatgatacgc cgcagcgccg gccggccggc
    38581 ctcagtcgcg gcggaagccg accggcacgg gcgggtcggt gaacatcgcg gtgtcgagga
    38641 actcccacca gtcctcttgg ctcacgcgct cggactccca gccttcgtcg gccttgagcg
    38701 tgccgagcgc ggcacggtag gcaccccacg cggcctcgtc ttcgaacgcg gcccagttct
    38761 cgagcacgta gacgccgccg acgatcgagt agtaccagcg gacctctttg accatcggca
    38821 ggtcgcggta gaaccactgc tcgcgcgcct cgagccagga ccagaactcc ttcatgttct
    38881 cgcgcgcacg acgcgcgagc cgcattcggt accgcagata gatcacacct cacccccgaa
    38941 cgtccggacg aagcacggcc cgccgcacgc cggcagcgga tcgccggcga cgacgaaagc
    39001 gtacgcggcc tgcgtcagat cgaacagctc cgcggtgacg gccgcgggcg cgccggcctc
    39061 gacgctctcg gccagccagg ccgcgtcgcg ttcgcctcgg cgcagccgtc gcgccagcct
    39121 gtttccgagc ggcgagggct cgaccgtatg cgcggcatcg tcgtgcagag acccgaacat
    39181 tgttttgacc atcggcgtcg gagcgccgga ctccgaggtt tcgcgccacc gggccgggcc
    39241 gtcgaagcgc gcgaacacca ggcactcttc ggcggcctcg gtccaggagc gcaccgaccc
    39301 ggccggcacg tatccccagt cgcccgcgcg atacgtcaca ccgctcatgt gcaggacgcc
    39361 gcggatgacg acgagctcct cggcggcctc gtagaagccg gataccggcc gcgaccagcc
    39421 cgccgggaag cgcacgaaga gcgtctgcgc gcgtgacgtc tcgtcgctgc ggagccgaac
    39481 gatcggtacg ggcgcggtgc cgccgggaat ggggaactcg gtccagccgg agaccgattc
    39541 cggcgtgatc acgggtggtg ggccgcgaga tcgctcatga gctgctcggc ctcttcgacg
    39601 gacacgtgcc gcaggccgaa gtcggcggcc agctcgtcgg tgaagtcgat ctggtgctgc
    39661 caagcgccgt tgacctcgtc cacgcagcgt ttgatctgat cgtcgtccag cggcatcgag
    39721 ttcatcttct cgtggtagga cttcaggaat ccgcgcgggt cctcgatgcg atcgaagcgg
    39781 aagaacgcgg ttccctgctc gtcgaacccg taggccttgt tcatgatccg caggttgatg
    39841 cgctgcgcga gcacattgcc gaggtatcgc agccattggt gcggcgcgaa cgcgggcgga
    39901 aagcgatcga tcgtcgagcg gatggcggcg acgtaggact cggtctgcgg cgacggcttg
    39961 acgcggtcac gccagtcctc gcccgccagg aaacgcatgt cggcgtcgat ggcggcactg
    40021 cggtgcagct ccggcgtgtg catgcggccc acgaccgagt cgtccttcag ggcctccgac
    40081 gtctcctcca gcgccgcgta gatgtaggag agccggccga gatactcgat gtaggcgtcc
    40141 ttgggaagct cggccttgaa gaacacgacc atgaagggcc ggcgctcggc ggcacgatgg
    40201 atctcggcgc tgtcgcgcat cagggattgc cacagcccgg gctcgtcccg ctcgctcacc
    40261 gctgcctcct cgggttcaag ataggggtcg ggcgcgacca ccccgccgac ggccgcgccc
    40321 cgaccctgcg tcgatcctac gcctgtgcgg cgacgagatg cttatcgccg agaagcgtcg
    40381 cgagctcgat gttgtattcg taagcgacca tgacctcttc aataatgagc tttttggtgg
    40441 ccgcgtctac cgggaggtca tcgaggatcg aacggtattg ctgcttgaac tcggcggcgt
    40501 cgccgatctc ggcgaagtcg tagtaggtca ggccttcgcc gttgagcttc cacgcgtcgc
    40561 gaagcgccgc cgcgatgaac aggccgccgg agaggtcggc catgtagcgc acgtaatggt
    40621 gcgcgacgaa ctgcgcaggg gatgcgttgt tgatccggtc gcaatactcc tggacgaccg
    40681 gcaggatcgg ataggtgcct ttccagtccg ggcccgcgaa gaacgccgcg tcggccgcga
    40741 cgtgctcgcg ccggtacagg tccttgaaga tcacgcgcga ggcgaccgga tcgtcgagca
    40801 tctgctcggt cttggtctcg agcgcgtcgt agatgtacca ggtctgcgcg agcaggtcgg
    40861 cgtagccttc cttgctgacg gtcgctttga cgagcgactg ctcgaacgga gagtactcgg
    40921 cgcgctgatg gtcgccccag gactcgcggc gcatcacgcg agagaacggc ttcgttgcga
    40981 tggcttcgtc gaccttctcg ccgaggcgag tggacgcctc ttcgacgcgc ttgaggatct
    41041 tggacatgac ctgctccccc tgggaggccg ggaacgccag cattgagtgg cttctttgcg
    41101 acgatctgtc gtcaacggta tcgggttcgc gacaacccgt caagaacata gtgacggatt
    41161 gtcggaaaaa gcctagggcc ggtagcccag gcggcgggag agccggtccg ttgcgtccag
    41221 aagcttcttg gccaggtcgg tggcctcgcc gcggaagcgc tccagcgggc cgcacacact
    41281 gatcacggcc gcaggcttgc cgtcgtggga gaagaccgga gcggccaccg agccggctcc
    41341 ctccatgcgc tcccccagcg acatcgcgta gccgcgatcg cggatgtcct tgagctcctt
    41401 cgcgagcgcc gagcggctcg tgatcgtcag tccggtcagc cgctcgagcg gctcggtcag
    41461 gtagtccgtc tgctcatcct cgtcgagata ggccaggaag gccttcgagg aggcccccgc
    41521 atgcagcgga acgctcagcc cgagctgcac caccatcttc acgtcgcggt cgggagtgac
    41581 ctgatcgacg tagacccgcc ggtcgcccgt gcgcagcgac aaggtcgctg tttcgtgggt
    41641 ctcgcgcgag agcgcttcca gcaccggccg ggccacacgc ggaacctccg tgcggttcac
    41701 gaacgcaagg ccgaggtgca gagcacgcgg cccgagggtg tagcgccgcg acgcctcgtc
    41761 gagctcgatg aagcccttgg cccggaagct cgacaggatc cggtagacga cggccttgga
    41821 caggtttagg gcttgcgcga tctcggtgac gccgagcgtg ggctcgtccg cgtcggcgaa
    41881 cagcgccagc acgtcggcgg ctcgctcgat cgcgacgatc gtctgtccct gcgcctcacg
    41941 cgccatgatc gccagcgtac cgcacagcga aacgaaatcg aaacgccgtt tcgcgtcgcc
    42001 gttcgtaccg tcgacgtgcg gcggatgccg gagaggggcc gggggccccg gaccctgtgc
    42061 aaccaagttg caatgaaggc gcgacggggg gccagggctg taacttggtt gcacagggtt
    42121 ccctagggac tcccccgggc tcgaaccggc ggcacgcgcg ggatcggctg cgcgaaaggc
    42181 taggtccggc ggaggtaaag gcctgggaag tcattggcga tctcgccctg tcggtacatc
    42241 gggatgccgc gtacgagcgt ggcggcgggc cagccgcgca cggcgacccc gctgaagggc
    42301 gtgtggtcct gcgcgctgcg gcagagctcg ggcgtgacga tctgctcgag cccgacgtcg
    42361 atgatcgcca gatccgcgtc ggcgccgacg gcgatcgttc ccttacgcgg atacaggttg
    42421 taggtgcgcg cgggtgcggc ggaagcgacc tgcgcgatgc gatcgagcgg gatgccgcgc
    42481 ttgtgatgcc cttcgctgat cagcaccgga tacagcagcg cggtgccacc gaagccgggc
    42541 agcgccggcc acaggtcctc gcccttggac tcctccatgc agcaggcgtg atcggacgcg
    42601 acggtgccga tgcggccgtc ggccaggccc tgccacagcg cctcgttgtc ttcggcgtcg
    42661 cggatcggcg ggttcacttt gccgccgagc cccttccctt cgagcatcgc cacgttgagc
    42721 gcgaggtgat gcacggtcac ctccaggttc gcgtccagcc cgtagcgatg ccgcacctcg
    42781 ttcgccttgt cgagcgcttc ccccgacgac agatgcagaa agttcgttcg cacgtgcgtc
    42841 gcgtcggcca gcacgccggc ctcttcgatc gagagctgct cggtcagggg cggccggccc
    42901 gcggcgtact cggccaggcc gcgcaccgaa ggatcctgct tcgcgcgctc gatgaacacg
    42961 cgaagcagct cggcctgctc gcagtgcagc gacagcgaga tacgtccgtc gtgtgcgaga
    43021 tccgcggccg agaccgcctc catgatctcg aacagatgac cgaggtcgta ctcctcggac
    43081 atggtgtagc ccttagcgtc gcgcgagtcg gccgacaggt tcaggccctt gtagaacatg
    43141 tagtacttga agctcgccac gcccatctcg gagaccagcc acgggatctc ggcgatgtgc
    43201 tcggccgtca tcggcgccaa gtgaaagccg tagtcaacct tggcgtgacc gtccacggcg
    43261 gcgagcacct cggggaagat ctggcgatac gggccggtct tgttcaaata gtggctgccg
    43321 gtgcggaagt acgagatcac cgtcgtgacg ccacccacca gcgacgaggt cgtctcggat
    43381 tcggtgtcct cggtcaggcc acggtagatg ccgaggtgat agtgcgagtc caccgcgccg
    43441 gggaagacgt gcttgccgcg cgcgtcgatg gcctcgtccg catcacccgg cccgaggtcg
    43501 ccggcgatcg acgcgatccg tccgtccttg atcgcgatgt cgccgcggac ctcaccgacg
    43561 aacggaatga ccagcgttcc tccggttacg acgacgtcgt agcgggccat gggaccgtca
    43621 cctctccttc gcgcaacgcg cgctcgaccg cgtccgcgcc cccttcggac agcacgcggg
    43681 acgcgttgtc gcggaacttc cgcgcgacct cgtcttcgga cagcggccgt cccgggcctc
    43741 cgcggttctg caagaccttc tgctccagat cccggccgtc gcgggtgcgc acgcgcacga
    43801 tcgcgggaag ctcgtcggga tagatcgcgt cgcacgcggg gtcggcgacg atctcgatcc
    43861 gctgcatcag cgcgcgccgg tccggatcgg ccgcgagcgc atcggtgaag tcgtccaatc
    43921 cgagcccgag ccccccgccg ccgtgcaacg cggccgcgac cgcgtacggc cccgagaact
    43981 gcgcctggta gccggtcgcg ggcgcacgct tcgcgtttat cggctcgcca atggtgcgaa
    44041 cggccggcgc cgcgacgccg accgtgatcg attcgacgtc ctccggcttc aagcccgcgt
    44101 cgcgcagcgc gatggccgcg tcgatcgcgg cgtgggtgaa atggttcgcc ggatacggct
    44161 tgaaatgaag ccggcggatc tcccattcgc cgggcgcgtg cagcggcgcc tcgtcgaact
    44221 cgcccgacag ccacgcctga tagaagccga agcggccctc gaacgccgtc ggcgcgccgg
    44281 tgatgccgcg cgaggccagc agcgcggcgg tcacgcccgc ctgcgcggcc cagccgcagt
    44341 gcaaacgctt cacggtcccg cccgcgcggt ttccttccag cacgcccgag cccatgctga
    44401 ccgcgatccc gagcgcatgc gcgatcccgt ccgcgtccaa cccgagcagc cgggccgatg
    44461 ccgcggccgc gccgagcgcg ccgcagatcg aggtcgcgtg ctggccgcgc tcgaagaaca
    44521 tcgactgcct tcgcgccgtg tcgtagccgg ccatgccggt gcggatgcag atctcgatcc
    44581 ccgccgcaac cgcatcggcg aacgcgtcgc gcgaggcgcc aacccgctcg gccaccgcga
    44641 acgccgcggg cacaacggag gccgacggat gaaggatcga cggcagatgc gtgtcgtcgt
    44701 agtcgagcga atgcgccagc acaccgttgg cgaacgcggc gctcgtcatc ggcagccgct
    44761 cgccgccgcc gaacaccgac gactgcgcgg cgcccccttg ctcgcgcagc atcgcgatca
    44821 gggactgcga ggtgtccagc ggtagcgccg cgaccgcgac cccgaccgtg tcgagcacgt
    44881 gcagccgcac ggccgcaaga acgccggccg ggaccgcggg cgcggcggcg aacgcgccga
    44941 gtcgcgcggc ggcggtgctc atgcgccgac gaccgcgagc ggccgaaccg gcgatccggt
    45001 cccgccgacg atcttgagcg gcgcaagcac gaacacgaac tcgtgcacgt ccgccgcggc
    45061 cagctcttcc agcgcgagcg tttccatgat gtggatgccg tgttcgacca ggagcacgcg
    45121 atgagcgggc agcagcgcgt gtcccgcgcc ggccggcaaa cgctcgaacg cgatcgagtc
    45181 ggcgcccgcg gcgcgaatcc cgcgagaggc cagccatttc gcggcctcct cgcccggacc
    45241 gggcacgccg gtcgcgcggc cgacgtaggc gtccgcatcg ggccagcggc gcccccagcc
    45301 ggtccggatc aacgcgacgt cgccttcgcg aagcgacacc tcgcacgctt ccaggtccgc
    45361 ggccgtgacc tcgtacgccg gatcgcacgc gtcgattccg agcgcgcgcg gaacgtccag
    45421 cagcacgccg cggcagaccc acggcgcgac ggtctcgatc ccgtggaccg agaaccggcc
    45481 gccgaccgac gcctccgccg catcgtgccc gccgaacagc ttgccgtcgt gcgagacgtg
    45541 cgcgagcgcg tcgatgtggg tgccgacgtg gccgccggtg acgatcatct cgttggcggc
    45601 gctgccaccg tcggcgcgtt tcatgtcgcc gtgccggcgc accagcgcca tccggaactc
    45661 gggatggttc ggcgactgcg gcatgcccgc gaacatctcg cggcccaggt cgaagatgcg
    45721 cacgcctcgc gcgaccgcat ccaacagcgg atcaggcaac gacgccacgc tcccgaagct
    45781 cggcgatccg ctcggccggc acgccgatcg agcccagcac ctcgtcggtg tcggcaccga
    45841 gagcacgtcc cgtccagcgg atcgatcccg gcgtgtcgct catccgaaac agcaggttct
    45901 gcatgcgaac ggatccgaag tcctcgtccg gcaccggcgt gatcatctcg gtcgcgcgca
    45961 cgtgcgggtc gtccatcagc ccggctacgt cgtagaccgg cgcgatcgcg gcctgtgcgg
    46021 cctcgaacgc atcgatcacc tcgtcgcgcg ttcgctcggc gatccacgcg gcgacgtagc
    46081 cgtcgagcag atctccgtgc cgcgcgcggc cgacgccggt ggtgaaccac ggctcgtcgc
    46141 acacctcggg atggccgacc agctccatca cacggcgcgc gatcgtatcg gccgacgtcg
    46201 agatcgcaac ccaggttccg tccttggtct tgtagacgtt gcgcggcgcg ttgttgtcgc
    46261 tgcggttgcc ccggcgcggc tgcacctcgc cgagctggtc gtagacgatc ggctgtggcc
    46321 cgagcacggt gacgatcggc tcgaggatcg agatgtcgat ctgctgtccg gttccgccgc
    46381 gcgcgtcgcg gtgatacagc gccatcatca ccg
//
LOCUS       DATLFO010000105         4927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_92544, whole genome shotgun sequence.
ACCESSION   DATLFO010000105 DATLFO010000000
VERSION     DATLFO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92544"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /locus_tag="VM841_03770"
     CDS             <1..2360
                     /locus_tag="VM841_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVL89335.1"
                     /translation="LRSIQIAQPFARRDPLPPTPPAPPDPRPDPGPHEPPPMPPGPEP
                     DSEPPKDFPPVDRTPVEPVARVEPSPRVEPAARADPSVGADAAPRAEPARRERPPPPT
                     RELPDIEEFVFAANRQAADDPGAEPLPAARDPGPRQDAAGNLSRRRLTALRAFCDALL
                     PAGGDLLWSARETGVAERIDAALPQLDPEVRKNLERLITLADRAPLASRGRHLSKLPP
                     EAAAAWVEKALDARNPIVRNAASLLKTIVANEFASTPQVEQAIGFTYSCETNDPVRDA
                     PPLEVISWPALDRDHLEECDVVVVGSGAGGAAAAKELAEAGLSVIVVEEGGLFTRRDF
                     SGPPWERVRRMYRANGTSVALGRPVIPIPTGKAVGGTTLVNSGTCFRTPDRILESWGS
                     EWGIDGIDPATMRPVFERVERILHVKPVPEEVIGENARIFRRGVEALGLHGAPLQRGI
                     QGCRGCGVCAFGCPSDAKQSTHVSYLPRAQRAGATIYARCAVERVVVEGGRAAGVEAG
                     ILDDRGRRVATLRIAAKAVVLSAGALHTPALLQASALANRSGMLGRNLRIHPAAGVGA
                     IFEEPVYSWRGTLQSYVVDDWHASEDLMIEVTAAVPGVSAGSMPGAGVKFKNMLARFP
                     YIATAGLFVSDTSQGRVRRRANREPLVTYRLNETDTRKIVVGLSRVADVFLAAGAKAV
                     FTGLASLPWVTSKDDIEDLREEAVKPSALKLTAFHPAGTARMGADPAATVVNPWGESH
                     DVPGLFVADASMLPGCPSVNPQVTIMAFATRTAAHLLRERADYFG"
     gene            2426..2896
                     /locus_tag="VM841_03775"
     CDS             2426..2896
                     /locus_tag="VM841_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018721371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVL89336.1"
                     /translation="MTLEIGKEAPDFEARSTAGQAVKLSDYRGKKHVLLVFYPLAFSR
                     TCTAEFCELRDESRHIVSDDVEVLGCSPDSAFTLKAWKAAEGYVNEFVSDFWPHGSIA
                     QKYGVFHEASGTSMRGTFLIDKDGILRWMEVQEDWQARDQQNWRKALADLGVSV"
     gene            2930..3181
                     /locus_tag="VM841_03780"
     CDS             2930..3181
                     /locus_tag="VM841_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89337.1"
                     /translation="MPCYRCGRVQEDPARGASPWARGVVADEQILICPECQQRHPAWS
                     GELRRCESCGSTRLQIQIGMIVCRQCGDAEEVRPVEAPF"
     gene            3165..3635
                     /locus_tag="VM841_03785"
     CDS             3165..3635
                     /locus_tag="VM841_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HVL89338.1"
                     /translation="MKLPSEGPVLLSACLAGVECTYKAEGKTRRWAVDLVAQGRGVPV
                     CPEVAGGLPVPRPEAEIRGGEGRDVLHGGARVVDVNGRDVTDAYLKGARIALTAARRT
                     GARIAVLKARSPACGCGAIYDGTFGGVLRDGDGVAAAALKGEGVTVVSDEEVSE"
     gene            3632..4021
                     /locus_tag="VM841_03790"
     CDS             3632..4021
                     /locus_tag="VM841_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVL89339.1"
                     /translation="MTALSPEVVALLRDRSFAHLAFVDEEGLPHVTPMWVDATDDGRV
                     LINTAEGRVKERVLTEGSAVALSVSMPDRPYSYVLIRGTIASRTTDGAVEAINKLSQK
                     YHGTDYALPDGMIRVTLLIEPRQVVVH"
     gene            complement(4018..>4927)
                     /locus_tag="VM841_03795"
     CDS             complement(4018..>4927)
                     /locus_tag="VM841_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89340.1"
                     /translation="SYVWPARHHAGARADATLPPMGAWFRLKAGFDISSYSTETRIIL
                     AAFKKHGLILADNGSNWYFGGAAEHGWSETVLNELKSITAGSFEAVDTSSLMVSPDSA
                     RIASGPSGAITASVSPGGGVWGTTFTASGVLSCDGAPVGGASLHVTRTYSGGPPVSLG
                     PVQTDALGSWSRSDVPSANAAYGASWSGGGECSGGAAAAASVTVRPGVIVNVWPSRLS
                     RGATATFTGKVLPAKPGKRVLLQVLNGSTGQWHSVAWATLDGSSGYRVGYRKTGPGWL
                     LFRVGYPTQDALHGWSLSRNIRVDWT"
BASE COUNT          710 a         1730 c         1734 g          753 t
ORIGIN      
        1 agcttcggtc gatccagatc gcccagccgt tcgcgcggcg cgacccgctg ccgccgacgc
       61 cgccggcgcc gcccgacccg cgccccgatc ccggcccgca cgagccgccg cccatgccgc
      121 ccggacccga gccggactcc gagccgccga aggactttcc gcccgtcgac cgcacgcccg
      181 tcgagcccgt ggcacgcgtc gagccctccc cgcgcgtcga gcccgcggca cgcgcggatc
      241 cctcggtggg tgcggatgcc gcaccccgtg ccgagcccgc gcgccgcgag cggccgccgc
      301 cccccacgcg cgagctgccc gacatcgaag agttcgtctt tgcggccaac cgccaggccg
      361 cggatgaccc cggggccgag cccctgcccg ccgcgcgtga tccgggtccg cgacaggacg
      421 ccgcggggaa cctgtcgcgg cgccgcctga ccgcgctgcg cgcattctgc gacgcgctcc
      481 tgccggccgg cggcgacctg ctttggtccg cgcgcgagac cggcgtcgcc gagcgcatcg
      541 acgcggctct gccgcagctc gaccccgaag tgcgaaagaa cctggaacgg ttgatcacgc
      601 tcgcggatcg cgcgccgctg gcctcgcgtg gccgtcacct ttcgaagctg ccgccggagg
      661 ccgcagcggc gtgggtcgag aaggcgctcg acgcgcgcaa cccgatcgtg cgcaacgcgg
      721 cgtcgctgct caagacgatc gtggccaacg agttcgcgtc cacgccgcag gtcgagcagg
      781 cgatcggctt cacctactcg tgcgagacga acgacccggt gcgtgacgcg ccgccgcttg
      841 aggtgatctc gtggccggcg ctggaccggg atcacctcga ggagtgcgac gtcgtcgtgg
      901 tcggctcggg cgcgggcggc gccgccgccg cgaaggagct cgccgaagcc ggcttgagcg
      961 tgatcgtcgt cgaggaaggc ggcctgttca ctcgccgcga cttctccggt ccgccgtggg
     1021 aacgcgtccg gcggatgtac cgcgcgaacg gaacctcggt ggcgctcggc cgtccggtga
     1081 ttcctatccc gaccggcaag gcggtcggcg gaacgacgct cgtcaacagc ggcacctgct
     1141 ttcgcacgcc cgaccggatt ctggagtcgt gggggagcga gtgggggatc gacggcatcg
     1201 atcccgccac gatgcggccc gtcttcgaac gcgtcgagcg catcctgcac gtcaagcccg
     1261 tgcccgagga ggtcatcggc gagaacgccc gcatcttccg ccgcggggtc gaggcgctcg
     1321 ggctgcacgg cgctccgctc cagcgcggca tccagggctg ccgcggctgc ggcgtgtgcg
     1381 cgttcggctg cccctccgac gccaagcagt caacgcacgt gtcgtatctg ccgcgggcgc
     1441 agcgtgccgg cgcgaccatc tacgcgcgat gcgccgtcga gcgcgtcgtg gtcgaaggcg
     1501 gacgggccgc cggggtggag gccggcatcc tcgacgatcg cggccggcgg gtcgccacgc
     1561 ttcgcatcgc cgccaaggcc gtagtgcttt cagccggcgc gctgcacacg ccggcgttgc
     1621 tgcaggcgag cgcgctcgcg aaccgctccg ggatgctggg acgcaacctt cgcatccatc
     1681 cggctgcggg cgtcggcgcc atcttcgagg agccggtcta ctcgtggcgc ggaaccttgc
     1741 agtcctacgt cgtcgacgac tggcatgcct ccgaagacct catgatcgag gtcaccgcgg
     1801 ccgtgcccgg ggtctcggcc gggtcgatgc cgggcgccgg cgtgaagttc aagaacatgc
     1861 tcgcgcgctt cccgtacatc gccaccgcgg ggctgttcgt gtcggacacc tcgcagggcc
     1921 gggtgcggcg gcgcgcgaat cgcgagccgc tcgtcaccta ccgcctcaac gagaccgaca
     1981 cgcgcaagat cgtcgtcggg ctctcgcgcg tcgccgacgt gttcctggcc gcgggcgcca
     2041 aggccgtctt cacgggcttg gcgtcccttc cgtgggtcac ctccaaggac gacatcgagg
     2101 atttgcgtga ggaagccgtg aagccctccg cgctcaagct gaccgcgttc cacccggcgg
     2161 gcaccgcgcg catgggtgcc gatcccgccg cgaccgtcgt caacccgtgg ggcgagtcgc
     2221 acgacgtgcc gggcctgttc gtcgcggacg cgtcgatgct tccggggtgt ccttcggtga
     2281 atccgcaggt cacgatcatg gcgttcgcga cgcgaaccgc ggcgcatctg ctgcgtgagc
     2341 gcgccgacta cttcggctga tttctcgcct gtttccgatc cgcgtattct ttcggtcact
     2401 caaacctaga ggaggatgcg gacccatgac gctcgagatc ggaaaggaag cgcctgactt
     2461 cgaggcgcgc tcgacggccg gccaggccgt gaagctgagc gactaccgcg gcaaaaagca
     2521 cgtgctgctc gtgttctatc cgctcgcgtt ctcccgcacc tgcacggccg agttctgcga
     2581 gcttcgcgac gagtcgcggc atatcgtctc cgacgatgtc gaggtgctcg gctgctcgcc
     2641 cgattcggcg ttcacgctca aggcttggaa ggccgccgag ggctacgtca acgagttcgt
     2701 ctcggacttc tggccacacg ggtcgatcgc gcagaagtac ggcgtgttcc acgaagccag
     2761 cggaacctcg atgcgcggaa cgttcctgat cgacaaggac ggcattctgc gctggatgga
     2821 agtgcaggag gactggcagg cgcgcgacca gcagaactgg cgtaaggcgc tggccgatct
     2881 cggcgtctcg gtctgacgat cgtttcgagg caccgataga ctggcgcgcg tgccttgcta
     2941 tcggtgcggc cgcgtccagg aggatcccgc ccgcggcgcc tctccgtggg cgcgcggcgt
     3001 ggtcgccgac gagcagatcc tcatctgtcc cgagtgccag caacgccatc cggcctggag
     3061 cggggagctc cggcgctgcg aatcctgcgg atcgaccagg ctccagattc agatcggcat
     3121 gatcgtgtgc cggcaatgcg gcgatgccga ggaggtgcgc cccgttgaag ctcccttctg
     3181 aggggccggt gctgctgtcg gcatgcctgg ccggcgtcga gtgcacctac aaggccgagg
     3241 gaaagacgcg ccgttgggcg gtggatctgg tcgcgcaggg acgaggtgtg ccggtgtgtc
     3301 ccgaggtcgc cggcggactt ccggttccgc gccccgaagc cgagatccga ggcggcgagg
     3361 gacgcgacgt tctccacggc ggggctcgcg tcgtcgacgt caacggtcgg gacgtcaccg
     3421 atgcctatct caaaggcgcg cgcatcgcgt tgactgccgc gcgccggacc ggcgcccgaa
     3481 tcgccgtgct gaaggcccgc agccctgcgt gcggttgcgg ggcgatctac gacggcacgt
     3541 tcggcggcgt ccttcgcgac ggagacggtg ttgcggccgc cgcgttgaag ggggaaggcg
     3601 tgaccgttgt ttccgacgag gaggtatccg agtgaccgcg ctctcgcccg aggtcgtggc
     3661 gttgctgcgc gaccggtctt tcgcgcatct ggcgttcgtc gacgaggagg ggctgcctca
     3721 cgtgacgccg atgtgggtgg acgcgaccga cgacggacgc gtgctgatca acaccgccga
     3781 aggccgcgtc aaggagcgcg tgctcacgga aggctcggcc gtggccctgt ccgtgagcat
     3841 gccggaccgg ccgtactcgt acgtgttgat ccgcggcacg atcgcttcac gcacaaccga
     3901 cggcgcggtc gaggcgatca acaagctgtc gcagaagtac cacggcaccg actacgcctt
     3961 gcccgacggc atgatccgcg tcaccctgct gatcgagccc cggcaggtcg tcgtccacta
     4021 ggtccagtcg acgcggatgt tgcggctgag gctccagccg tgcagagcgt cctgggtggg
     4081 gtagccgact cggaacagca gccagccggg gccggtcttg cggtagccga cacggtagcc
     4141 ggatgagccg tccagtgtgg cccacgcgac gctgtgccac tggcccgtgc tgccgttgag
     4201 gacttgcagc agcacgcgct tgccgggctt ggcgggcagc actttgccgg tgaaggtcgc
     4261 ggttgcgccg cgcgacagcc gtgagggcca cacgttgacg atcacgccgg gacgtacggt
     4321 caccgacgcc gccgccgccg cgccgccgct gcactcgccg ccgccactcc acgatgcgcc
     4381 gtaggccgcg ttcgcggacg gaacgtcgct gcgcgaccat gaacccagcg catcggtctg
     4441 caccgggccg agcgagaccg gcgggccgcc gctataggtt cgcgtgacgt gaagcgatgc
     4501 gccgccaacc ggcgcgccgt cgcacgacag cacgccgctc gcggtgaacg tggtgcccca
     4561 cacaccgccg ccgggtgaga ccgacgcggt gatcgcgccg gacggcccgg acgcgatccg
     4621 cgccgagtcg ggcgagacca tcagcgacga ggtgtcgacg gcttcgaaac tgcccgccgt
     4681 gatcgacttc agctcgttca acaccgtctc gctccagccg tgctcggcgg cgccgccgaa
     4741 ataccagttc gatccattgt cggccaggat caagccgtgc tttttgaatg cggccaggat
     4801 gatgcgggtc tcggtggagt agctcgagat gtcgaacccg gctttgagcc ggaaccatgc
     4861 gcccattggc ggcagcgtcg catcggcgcg tgcgcccgcg tgatgccgtg ccggccacac
     4921 gtacgag
//
LOCUS       DATLFO010000106        11069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_140665, whole genome shotgun sequence.
ACCESSION   DATLFO010000106 DATLFO010000000
VERSION     DATLFO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140665"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VM841_03800"
     CDS             complement(<1..913)
                     /locus_tag="VM841_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89341.1"
                     /translation="MRDGATGSNRAPEARPRTGPGSRELAAGSGRLAVFAMVRAGIPL
                     FALIGLASGPTLGIDVRRIAALLTAQVAMAGATQALAIRRRESAAAIVWLGIVADLVV
                     ITALCAVTGGASGPLVFLYTIEAVAAGILLESRVGLRVLGMSMAAIIGLDIAASRGLI
                     EGEAGGVQALIAVAVLWTTAGGAIGFSMFNEREIRRHSAQLQTIRRITLDIEDSLSIP
                     EIMADLCRGVVDEFNFSQAAVLVRRGDGLTVAGGHNVTGSSSQTLPIRGPLADAPADQ
                     HRRLAEVELVHHAAAQVRHDLGDRQRVL"
     gene            complement(888..1529)
                     /locus_tag="VM841_03805"
     CDS             complement(888..1529)
                     /locus_tag="VM841_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89342.1"
                     /translation="MRGTRFGGLLAAGALTLTLTTGTGMAATEDLTSGITGATQDVVP
                     SILPATITPLPDPAAVPGGNEIDLPGVSPPAMGGGDPVPASGGGGGSGGGSGGSGSGS
                     GDAPGPGAGHGGGGSSRTDGGGASDAHGGTRGGTGTGEPGSERGLAPAATLTGSAGTL
                     ATNIGRLAKPLGLPLGLAVVAAVMLLIATRRPVALRKVEEEGMWGYEGRSNRL"
     gene            1769..2410
                     /locus_tag="VM841_03810"
     CDS             1769..2410
                     /locus_tag="VM841_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89343.1"
                     /translation="MTSRIKVLIVDDHRVFAEALTLALGKESDIQVADPPALTGEDAV
                     HAVQEFEPDVVLMDVGLPGIDGIEATRRIREARPGTAVVILTSEAGASRLPAAAAAGA
                     CGYLDKTVALDDLGAAVRAAFAGQILLPEASPAGAADAPALTAPFDMLTPREQEVLEL
                     LAEGLPNRDIARRLVLSVRTVDTHVHNLLIKLGVHSKLEAVVKAMRAGVIGRR"
     gene            2459..2986
                     /locus_tag="VM841_03815"
     CDS             2459..2986
                     /locus_tag="VM841_03815"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HVL89344.1"
                     /translation="MFPDESAEIVGAGAPTQQQPQQPPQPHEGSVEQPAKLLRIAVMV
                     REMLEEVRRAPIDEYAREHLSKIHTGVLEELKSGLSGPLREELDRITMPLDGENPPTE
                     SEIRIAQAQLLGWLEGLFQGIQAAIATQQMAAAAQLEEMRRRGLPPGAQPRGMPGSPS
                     PAENGPPGERPGQYL"
     gene            complement(2988..3767)
                     /locus_tag="VM841_03820"
     CDS             complement(2988..3767)
                     /locus_tag="VM841_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89345.1"
                     /translation="MSKAAHSDAALVARQFRAEWASFWRNPAAAGFTFVFPIMFMVIF
                     NLLFDDPYTGRGPRVSAATFFTPALITFSLVSACFTNIAIGVVFAREEGVLKRVRGTP
                     LPPWIYLAGRIVNAVVVALILVAIMLAFGTLVYGVELPGSTMPAFVATLLIGAASLCA
                     LGLAATTLVPNEDAAPPIINLMVLPLLFVSDVFIPLEDAPGWLTTLADAFPIRPLATS
                     LIDSFIGGGSGWHPRSLAVVTLWGLAGVAVAARRFRWEPKR"
     gene            complement(3764..4678)
                     /locus_tag="VM841_03825"
     CDS             complement(3764..4678)
                     /locus_tag="VM841_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89346.1"
                     /translation="MSASAAGPAIQVRNLAKRYGEIEAVRGIDLSVERGEVFALLGPN
                     GAGKTSTVEILEGHRRRSGGEVDVLGFDPARGGRAFRSRIGVVLQQTGVEKYLTVAEV
                     IELFRGYYPAPLPLDEIIDVVGLREKRDERVRRLSGGQQRRLDVAVGLAGNPDLLFLD
                     EPTTGFDPGARRNAWDMIRNLKALGKTIFLTTHYMDEAQELADRVAVIAGGRIVAEGS
                     PDQIGGRDTAETLIRFRTPARPLPKAVAAGASVHGDTTAIGTATPTKTLAALTAWAVR
                     NSIELEDLQVTRPTLEDVYLRLAGAQGE"
     gene            4704..5348
                     /locus_tag="VM841_03830"
     CDS             4704..5348
                     /locus_tag="VM841_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVL89347.1"
                     /translation="MVECAAMPSEPIPEKPREVKGLIAPIIRVALAAPWRGALRLLLW
                     MRVTADHLTMASLALNIWIAVLLAQGNRLLPGLLLLPAGLLDVFDGSVARARGTSGNR
                     GAYLDALLDRASDAAVLGGLFLSLTWQARSLQAALALGALAVSLFVSHVRAEAAARGV
                     TMGEGLFARLERYVALMIGLVVPGALPWALAALVVLGGVTVIQRILIVRKALSG"
     gene            complement(5345..5764)
                     /locus_tag="VM841_03835"
     CDS             complement(5345..5764)
                     /locus_tag="VM841_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89348.1"
                     /translation="MPEVAIVAQAEVAPLLEAMRADGLDVIPGEGTWIGARDAGGSLA
                     GVARIFDRDGIAALDDVWVAPAHRRAGVGSALVAHARTMRRPLWLIADEIDVGFYAAR
                     GFATEADTALPAGLAAHYASKDEWPAPDHRHVAMRAI"
     gene            5841..7637
                     /locus_tag="VM841_03840"
     CDS             5841..7637
                     /locus_tag="VM841_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89349.1"
                     /translation="MTAPGEARARTPVPAGAPVERVLEAVSHSTRAAVVSVAAAATAA
                     AAGVLPALMVRRIVDHALPGSRQGALVLLAAGMVAFAVLAATASAIRAYAATVVAQRV
                     AADLRTRVYESVRRQSLGYFGDARAGDLTGMLTGGVDAAVSGVETASSHIASVFTLVA
                     TLIAMTMLSPLLTLVAVAMVPLLVLPARRLERVRTEAAGRDKERLAGLSGLAQEALGA
                     PGAALAKTFGRDGHEGRRFAERARAVRDTAIADALASRWPAVARQGVAALAPAAVWLL
                     GGRRLIDGTMTLGTVIAVTMLQVRLPGPAEQLLTVRADAAGSLARFDRIFEHLDLVPE
                     VAERPGATALASVQGRITFEDVSLEARGGGGGLRGLSLEVPSRRMLAIVGTAGSGTSL
                     IAPLVARLHDPDHGSVLLDGHDLRELSLAAIADAVAVIAREPFLFHATVRDNILYGRP
                     DAPESYVAEAAKAARVHDFILTLPDGYDTIVGEGGHPLPAAETHLIAIARTLLKNPAV
                     VLVDEPAGVFGAEDERYVVRALATLLKGRTVLIVARRPATVMAADQILVLDAGRIAER
                     GTHDELIARGGLYARLYREQFATGPRLADVLA"
     gene            7681..8079
                     /locus_tag="VM841_03845"
     CDS             7681..8079
                     /locus_tag="VM841_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVL89350.1"
                     /translation="MLRRILVTSDGSERAGRAVAFAADLAGRYGAELTVLHVRVGDNV
                     VPQRDLDKQAAAAGATVAVVEDSAPAEAICRHAEENGCDAIVLGNYGMSDRKQFLLGN
                     VPNRVSHQAQCTVIIVDTRDPKERKRHRGR"
     gene            8090..9853
                     /locus_tag="VM841_03850"
     CDS             8090..9853
                     /locus_tag="VM841_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HVL89351.1"
                     /translation="MNDHDDVAAPGDPDSPAAQAPAAASGPPPPEPRTAKAAKAPRPR
                     KPRPPRAAARSKKDAEPDGGSLTRRVARIGTVLARHGVRDIFTGGLNTADPAKLRAGA
                     IAFRDALQELGPTFSKLGQILSTRPDLLPAEFIAELESLQEKVTPLTEAEVVGAIETS
                     LGVPWEDAFESIDSEPLAAGTIAQVHRAVLEDGEAVVVKVQRPNAAREILQDLELLRM
                     FAEQTGDLTAIRRIVDVPAIVDHLATSLERELDFTREASNIKRMQEVLSGFDRLGVPE
                     VYDLFTSKTLLVLRFVEGVPLRQAPDVPERSEAAKQLLEAYYHQVLSEGFFHADPHPG
                     NMRWGSDNKIYLFDLGMVGELDPPVRDAMMLVVLALWQEDSRFLADSVLSLAEHDDVD
                     TDAFAADVDRVMRSFTGKSLRDIQLGPLLQEMTAISMQHGVRLPASLALTAKALAQMQ
                     LATAELMPDLDPFSVAGRYMMRHIAGRVGGALAPGKVLYEASKVASRAGRILRSVESL
                     AASMKGGRLQVQFRGTEGFEETIRRAGRRVALSLGATATITASAIAGTSERIPDWLPV
                     TGVATGAMLLGMLLADILSRK"
     gene            complement(9854..9985)
                     /locus_tag="VM841_03855"
     CDS             complement(9854..9985)
                     /locus_tag="VM841_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89352.1"
                     /translation="MGGIRSTFRRFERAVLRTLMRAGAKFADRRVAKEVESRRSKSG"
     gene            10253..11050
                     /locus_tag="VM841_03860"
     CDS             10253..11050
                     /locus_tag="VM841_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL89353.1"
                     /translation="MRRLARYSIALILATAVSIAGVQVVQAASGEENGFVSRINSERS
                     KRGIHTLVWAEDLAQVARRHSQRMASQKNLHHNPNLRSEVDGWEVVGENVGYGPGVED
                     LHQAFMDSQSHRANILDRSYTQVGVGTVWRDGLLWVTQVFRKPVSSSNTSNGSGPSAS
                     GSSSAPRKASKPKAAAKPKPAAPRPVPAAPAAPIHPPSTPAAELTRRMIETLLGGQEP
                     VSGSVEAADFSLEPASVGSGSAGMSLRSFAELAGRVNRRLLEQAAGR"
BASE COUNT         1565 a         4048 c         3881 g         1575 t
ORIGIN      
        1 cgaggactcg ctgtcgatcc ccgagatcat ggcggacctg tgccgcggcg tggtggacga
       61 gttcaacttc agccaggcgg cggtgctggt ccgccggggc gtcggccagc ggaccgcgga
      121 tcggaagcgt ctgcgacgac gagccggtga cgttgtgccc gcccgcgacc gtgaggccgt
      181 cgccccggcg gaccagcacc gccgcctggc tgaagttgaa ctcgtccacc acgccgcggc
      241 acaggtccgc catgatctcg gggatcgaca gcgagtcctc gatgtcgagc gtgatgcgtc
      301 ggatcgtctg gagctgggcg ctgtggcggc ggatctcgcg ctcgttgaac atgctgaacc
      361 cgatcgcgcc gcccgcggtc gtccacagca ccgcgaccgc gatcagggcc tgcacgccgc
      421 cggcttcgcc ctcgatcagc ccgcggctgg cggcgatgtc gagcccgatg atcgcggcca
      481 tcgacatgcc gagcactcgc agccccacgc gcgactccag caagatgccc gccgcgaccg
      541 cctcgatggt gtagaggaac acgagcggtc cggatgcacc gccggtgacc gcgcacagcg
      601 cggtgatcac gacgagatcg gcgacgatgc ccagccacac gatcgcggcg gccgactcgc
      661 ggcgacggat cgcaagggcc tgcgtggcgc cggccatcgc gacctgcgcg gtgagcagcg
      721 ccgcgatgcg gcgcacgtcg atccccaagg tcggtccgga agcaagaccg atcagcgcga
      781 acagcgggat gccggcccgg accatcgcga agacggcgag ccggcccgat cctgcggcga
      841 gctcgcgcga gccgggccct gtgcgcggcc gggcctcggg ggcgcggtta gagccggttg
      901 ctccgtccct cataccccca catgccttcc tcttcgacct tgcgcagcgc gaccggtcgt
      961 cgcgttgcga tcagaagcat cacggccgcc acgaccgcca gcccgagcgg aagcccgagc
     1021 ggcttggcga gccggccgat gttggttgcc agcgtccccg cggagccggt gagcgtcgcc
     1081 gccggcgcga ggccgcgttc ggagccgggc tcgccggttc cggtgccgcc gcgcgtcccg
     1141 ccgtgcgcgt cggatgcgcc gccgccgtcc gtgcggctcg atccgccgcc gccgtgcccg
     1201 gcaccggggc cgggcgcgtc gccgctcccg gatccgctgc cgccgctgcc gcccccggag
     1261 ccgcctcctc cgccgctcgc gggcacgggg tcgccgccgc ccatcgcggg cggcgagacg
     1321 cccggaagat cgatctcgtt gccgcccggc actgccgcgg gatcgggaag cggcgtgatg
     1381 gttgccggca ggatcgacgg gacgacgtcc tgcgtcgcgc cggtgatccc cgaggtgaga
     1441 tcctcggtcg cggccatgcc cgttcccgtg gtcagcgtga gcgtcagcgc tcccgccgcg
     1501 agcagccctc cgaaccgtgt gccgcgcaat gtttctcctt cgcccaggtg ttcgacgcga
     1561 ggaacgggtg ttcgggcgcg tgaccgcccg gtccggaatc cgccctgcac gggggggacg
     1621 ggtcccgaga ctatgatcgt cctcgaactg cgttgctctt gttcgttcgg gtcgctgctt
     1681 ctagttctgg atggcccggg cgagccattt ccatagtcaa cccccgtcgg ggaggggaca
     1741 aaccggcaca gaacaggcgc atttatccat gacctcacgc attaaggttc tcatcgtcga
     1801 cgaccatcgc gtgttcgccg aggcgctcac gctcgcactg ggcaaggaaa gcgacatcca
     1861 ggtcgcggac ccgcccgcgc tgaccggcga ggacgccgta catgccgttc aagagttcga
     1921 gccggacgtg gtcctgatgg acgttggtct gcccgggatc gacgggatcg aggcgacccg
     1981 tcgcatccgt gaggcgcggc ccggtaccgc cgtcgtcatc ctgacttcgg aggcgggcgc
     2041 ctcccggctg ccggctgccg ctgctgccgg cgcctgcggc tacctggaca agacggtcgc
     2101 cctcgatgac ctcggcgcgg cggtcagggc cgcgttcgcc ggacagatcc tgctgcccga
     2161 ggcctccccc gcgggcgcgg ccgacgcgcc cgcgctcacg gccccgttcg acatgctgac
     2221 cccccgcgag caggaggtgc tcgagcttct ggccgaaggc ctgccgaacc gggacatcgc
     2281 gcggcgtttg gtcctttcgg tccgcacggt cgatacgcac gtgcacaacc ttttgatcaa
     2341 gctcggcgtg cactccaagc tcgaggccgt ggtcaaggcc atgcgcgcgg gcgtgatcgg
     2401 acgacgctag tcgaagcgag aggcgtcgtc ggcggcgcgg gtgggtagac tggcgcccat
     2461 gttccccgac gagtccgcag agattgtcgg cgccggcgcg cccacccagc agcagcccca
     2521 gcagccgccg cagccgcacg aaggctcggt cgagcagccc gcgaagctgc tgcggatcgc
     2581 ggtgatggtg cgcgagatgc tcgaagaggt gcggcgcgcg cccatcgacg agtacgcgcg
     2641 tgagcacctg agcaagatcc acaccggcgt gctcgaagag ctcaagagcg gcctgtccgg
     2701 cccgctgcgt gaggagctcg accggatcac catgccgctc gacggcgaga acccgcccac
     2761 cgaatccgag atccgcatcg cgcaggccca gctgctcgga tggctcgagg gactgttcca
     2821 gggaatccag gccgcgatcg cgacccagca gatggcggcc gccgcgcagc tcgaagagat
     2881 gcggcgccgc gggctgcctc ccggcgcgca gccccgcggc atgcccggca gcccgtcgcc
     2941 ggccgagaac ggcccgccgg gcgagcggcc cggccaatac ctttagctca gcgcttcggc
     3001 tcccagcgga accggcgtgc cgcgaccgcc acgcccgcaa ggccccacag cgtcacgacc
     3061 gcgaggctgc gcggatgcca gccgctgccg ccgccgatga acgaatcgat cagcgaggtc
     3121 gccagcggcc ggatcgggaa tgcgtcggcg agcgtggtga gccagccggg cgcatcctcg
     3181 agcgggatga acacgtccga gacgaacagc agcggcagga ccatcaagtt gatgatcggc
     3241 ggcgccgcgt cctcgttcgg caccagcgtg gtcgccgcga gcccgagtgc gcacagcgac
     3301 gccgcgccga tcagcagcgt cgccacgaac gcgggcatgg tcgagcccgg aagctcgacg
     3361 ccgtagacga gcgtgccgaa cgcgagcatg atcgccacca ggatcagcgc gacgacgacc
     3421 gcgttgacga tgcgtccggc cagatagatc cacggcggca gcggcgtgcc gcgaacgcgt
     3481 ttgagcacac cctcctcgcg cgcgaacacc acgccgatcg cgatgttggt gaagcaggcc
     3541 gacaccagcg agaacgtgat cagcgcgggc gtgaagaagg tggccgcgct cacgcgcggg
     3601 ccgcggccgg tgtagggatc gtcgaataga agattgaaga tgaccatgaa catgatcggg
     3661 aagacgaacg tgaaccccgc cgcggccggg ttgcgccaga acgaggccca ttccgcgcgg
     3721 aactggcgcg ccacgagcgc cgcgtccgaa tgcgccgcct tgctcattcg ccctgcgctc
     3781 cggccagccg aaggtagacg tcctcgagcg tgggacgcgt cacctgcaga tcctcgagct
     3841 cgatcgagtt cctgaccgcc cacgccgtca gcgccgccag cgtcttggtc ggcgtcgcgg
     3901 tgccgatcgc ggtggtgtcg ccgtgaaccg aggcgccggc ggcgaccgcc ttgggcagcg
     3961 gacgcgcggg cgtgcggaac cggatcagcg tctcggccgt gtcgcgtccg ccgatctgat
     4021 cgggcgaacc ctccgcaacg atgcggccgc ccgcgatcac cgcgacgcgg tccgcgagct
     4081 cttgggcctc gtccatgtag tgcgtggtga ggaagatcgt ctttccgagc gccttgaggt
     4141 tgcggatcat gtcccaggcg ttgcggcgcg cgcccggatc gaacccggtg gtgggctcgt
     4201 ccaggaacag aagatcggga ttgccggcga ggcccaccgc cacgtccagg cggcgctgct
     4261 ggccgcccga gagccgccgc acgcgctcgt cgcgcttttc ccgcagcccg accacgtcga
     4321 tgatctcgtc gagcgggagc ggggcggggt agtagccgcg gaacagctcg atgacctcgg
     4381 cgacggtcag gtacttctcg acgccggtct gctgaagcac cacgccgatg cggctgcgga
     4441 acgcgcggcc gccgcgcgcc gggtcgaagc cgagcacgtc gacctcgccg ccgctgcgcc
     4501 gccggtgccc ttcgaggatc tcgaccgtgc tggtcttgcc ggccccgttc gggccgagca
     4561 acgcgaagac ctcgcctcgc tcgacgctca ggtcgatgcc gcgcacggcc tcgatctcgc
     4621 cgtagcgttt cgccaagttg cggacctgga tcgccgggcc ggccgcggat gcgctcatcg
     4681 agtggggcag gatacccgct accgtggttg aatgcgcggc gatgccgtcc gagccgatcc
     4741 ccgagaagcc gcgcgaagtc aagggtctga tcgcgccgat cattcgcgtc gcgctcgcgg
     4801 cgccgtggcg cggcgcgctc cggcttctgc tgtggatgcg cgtcaccgcc gaccatctca
     4861 ccatggcctc gctcgcgctc aacatctgga tcgcggttct gctcgcacaa ggcaaccggc
     4921 tgctgcccgg tctgctgctg ctgccggccg gattgctcga tgtcttcgac ggatcggtcg
     4981 cgcgggcgcg cggcaccagc ggcaatcgcg gcgcctactt ggacgcgctg ctggaccgcg
     5041 cgtcggacgc ggcggtgctc ggtggcctgt tcctgtcgct gacctggcag gcacggtcgc
     5101 tgcaggcggc gctcgcgctc ggcgcgctcg ccgtgtcgct gttcgtctcg cacgtgcgcg
     5161 ccgaggccgc cgcgcgcggc gtcaccatgg gcgaagggct tttcgcgcgg ctcgagcgct
     5221 acgtggcgct gatgatcggg ctggtggtgc ccggcgcgct gccgtgggcg ctggcggcct
     5281 tggtggtgct cggcggggtc accgtgatcc agcgcatcct gatcgttcgc aaggcgctgt
     5341 cgggctagat cgcccgcatc gcgacgtggc ggtgatccgg tgccggccac tcgtccttgg
     5401 acgcgtagtg cgccgcgagc ccggccggca gagccgtgtc ggcctcggtc gcgaagccgc
     5461 gcgcggcgta gaagcccacg tcgatctcgt cggcgatcag ccagagggga cggcgcatcg
     5521 tcctcgcgtg cgccaccagc gccgagccga cacctgcgcg gcgatgcgcc ggcgccaccc
     5581 acacgtcgtc cagcgccgcg attccgtccc ggtcgaagat ccgggcgacg ccggcgagtg
     5641 atccgccggc gtcccgggca ccgatccacg tcccttcgcc ggggatcacg tcgaggccgt
     5701 ctgcgcgcat cgcctcgagc agcggcgcga cctcggcctg cgcgacgatc gcgacctcgg
     5761 gcacggccca agcataggcg cgaggcgcgg gaagacgggc gggcgtgacc cgtgacgggc
     5821 cgggcggcta cgctggcggg atgacggctc cgggcgaggc tcgcgcacgg acgccggttc
     5881 cggccggcgc gcccgtcgag cgcgtcctgg aggcggtgtc ccattcgacg cgcgccgccg
     5941 tcgtgagcgt cgccgctgct gcgaccgccg ccgccgccgg cgtgctgccc gccctgatgg
     6001 tgcgccgcat cgtcgatcac gcgctgcccg gtagccgcca gggtgcgctc gtgctgctcg
     6061 cggccggcat ggtcgccttc gcagtgctgg ccgcgacggc gtcggcgatt cgcgcgtacg
     6121 cggcgaccgt cgtcgcgcag cgcgtcgccg cggaccttcg cacgcgtgtc tacgagtcgg
     6181 tgcggcgcca aagcctcggc tacttcggcg acgcgcgcgc cggcgacctg accgggatgc
     6241 tcaccggcgg cgtggacgcc gcggtctccg gcgtcgaaac cgcgtcctcg cacatcgctt
     6301 cggtgttcac gctcgtcgcg acgctgatcg cgatgacgat gctgagcccg ctgctgacgc
     6361 ttgtggccgt ggcgatggtg ccgctgctcg ttcttcccgc acgccggctc gagcgtgtgc
     6421 gcaccgaagc ggccggccgc gacaaggagc ggctcgccgg cttgtcgggt ctggcccagg
     6481 aagcgctcgg cgcgcccggc gccgcgctcg caaagacgtt cggacgcgac ggacacgaag
     6541 gccgccgctt cgccgagcgc gcgcgcgccg tgcgtgacac ggcaatcgcc gacgcgctcg
     6601 cgagtcgctg gcccgccgtc gcgcgtcaag gcgtcgccgc actcgctccg gccgccgtct
     6661 ggctgctcgg cgggcgcagg ctgatcgacg gaacgatgac cctcggcacg gtgatcgccg
     6721 tcacgatgct gcaggtgcgt ctgccgggcc cggccgagca gcttctgacc gtccgcgccg
     6781 acgcggccgg atcgctcgcg cggttcgacc gcatcttcga gcacctcgac ctggtgccgg
     6841 aggtcgccga acgcccgggc gcgaccgcgc tggcctcggt gcagggccgc atcacgttcg
     6901 aggacgtctc gctcgaggcg cgcggcggcg ggggcgggct gcgcggcctg tcgctggagg
     6961 tcccgtcgcg acgcatgctc gccatcgtcg gcaccgcggg atcgggcacg tcgctgatcg
     7021 cgccgctcgt cgcgcggctg cacgaccccg atcacggctc ggtgctgctc gacggccacg
     7081 atctgcgcga gctgtcgctc gccgcgatcg cggacgcggt cgcggtgatc gcgcgcgagc
     7141 cgttcctgtt ccacgcaacc gtgcgcgaca acatcctcta cggccgtccc gacgcgcccg
     7201 agtcctacgt cgcggaggcc gccaaggcgg cgcgcgtcca cgacttcatc ctgaccttgc
     7261 ccgacggcta cgacacgatc gtcggggaag gcgggcatcc gcttcccgcc gccgagacgc
     7321 atctgatcgc catcgcgcgc acgcttttga agaacccggc ggtcgtgctc gtcgacgagc
     7381 cggcgggcgt gttcggcgcc gaggacgagc gctacgtcgt gcgggcgctg gcgacgctgc
     7441 tcaagggacg gaccgtgctg atcgtggccc gccgtcccgc gacggtgatg gcggcggacc
     7501 agattctggt gctcgacgcc ggtcgcatcg ccgagcgcgg aacccacgac gagctcatcg
     7561 cccgcggcgg cctgtacgcc cggctctacc gcgagcagtt cgcgaccgga ccccgcttgg
     7621 ccgacgtgct cgcatagccg tgttgccgcg gggcctgcgg acgggttgaa tggcggctcc
     7681 atgttgcgac gaatcctggt gaccagcgac ggaagcgagc gtgccggccg tgccgtggcc
     7741 ttcgccgcgg atctcgccgg ccgctacggg gccgagctga ccgtgctgca cgtgcgggtc
     7801 ggcgacaacg ttgttccgca gcgggacctc gacaagcagg cggccgcggc cggcgccacc
     7861 gtcgcggtcg tggaggacag cgcgcccgcc gaggcgatct gccgccacgc cgaggagaac
     7921 ggctgcgacg cgatcgtcct cggcaactac ggcatgagcg accgcaagca gttcttgctc
     7981 ggcaacgtcc cgaaccgcgt ctcgcatcaa gcgcagtgca cggtgatcat cgtcgacacc
     8041 cgcgatccga aggagcgcaa gcgtcaccgc ggccggtaga cgctgagcga tgaacgatca
     8101 cgacgacgtc gccgcacccg gcgatcccga ttccccggcc gcccaggctc ccgcggccgc
     8161 atccggtccg ccaccgcccg agccgcgcac ggccaaggcc gcgaaagccc cgaggccgcg
     8221 caagcctcgt ccgccgcgcg cggccgcccg gtccaagaaa gacgccgagc ccgacggcgg
     8281 ctcgctcacg cgtcgcgtcg cgcgcatcgg caccgtgctc gcccgccacg gcgtgcgcga
     8341 catcttcacc ggcggcctga acaccgccga tccggcgaag ctgcgcgccg gcgcgatcgc
     8401 gttccgcgac gcgctccaag aactcggccc gaccttctcc aagctcggcc agatcctttc
     8461 gacgcgcccc gaccttctgc ccgccgagtt catcgccgag ctcgagtcgc tgcaggagaa
     8521 ggtcaccccg ctgaccgaag ccgaggtcgt cggcgcaatc gagacctcgc tcggcgtgcc
     8581 gtgggaagac gcgttcgagt ccatcgactc cgagccgctc gcggccggca cgatcgcgca
     8641 ggtgcatcgc gcggtgctcg aggacggcga ggccgtcgtg gtcaaggtgc agcgtcccaa
     8701 cgccgcgcgc gagatcctcc aagacctcga gcttcttcgc atgttcgccg agcagacggg
     8761 cgacctgacc gcgatccggc gcatcgtgga cgtgcccgcg atcgtggatc acctcgcgac
     8821 ctcgctcgag cgcgagctcg acttcacgcg cgaggcctcc aacatcaaac gcatgcagga
     8881 ggtcctgtcg ggcttcgacc ggctcggcgt gcccgaggtc tacgacctgt tcacctccaa
     8941 gaccctgctg gtgctgcgct tcgtcgaggg cgtgccgctg cgtcaggcgc ccgacgtgcc
     9001 cgagcgcagc gaggcggcca agcagttgct cgaggcctac taccaccagg tgctctcgga
     9061 aggcttcttc cacgccgatc cgcatccggg caacatgcgc tggggcagcg acaacaagat
     9121 ctacctgttc gatctgggca tggtcggcga gctcgatccg cccgttcgcg acgcgatgat
     9181 gctggtcgtg ctcgcgctct ggcaggagga ctcgcggttc ctggccgaca gcgtgctgtc
     9241 gctggccgag cacgacgacg tggacaccga cgccttcgcg gcggacgtgg accgcgtgat
     9301 gcgctcgttc accggcaagt cgctgcgcga catccagctc ggcccgctgc tgcaagagat
     9361 gaccgcgatc tcgatgcagc acggcgtgcg gctgccggcg tcgctggcgc tgaccgccaa
     9421 ggcgctcgcg cagatgcagc ttgcgaccgc cgagctgatg cccgacctgg acccgttctc
     9481 ggtcgccggc cgctacatga tgcgccacat cgccggccgc gtcggtgggg cgctcgcccc
     9541 gggcaaggtt ctctacgagg cctccaaggt cgcgtcccgg gcgggacgga tcctgcgctc
     9601 ggtcgaatcg ctggccgcga gcatgaaggg cggccgcctg caggttcagt tccgcgggac
     9661 cgaaggcttc gaggagacca ttcgccgcgc cggtcgccgg gtcgcgctct cgctcggcgc
     9721 gacggccacg atcaccgcgt cggcgatcgc aggaaccagc gagcgcatcc cggattggct
     9781 gccggtcacc ggcgtggcca ccggcgcgat gctgctgggc atgctgctgg ccgacatcct
     9841 cagccgtaag tagctatccg ctcttggaac ggcgcgattc gacctctttg gccacccgcc
     9901 ggtcggcgaa cttcgcgccc gcgcgcatga gcgttcgcag cacggctcgc tcgaagcgcc
     9961 ggaacgtgga tcggattccc cccatgacct cggaatggta gcgtcccggc gcgctacccg
    10021 ctcgccggtt caaaagggac gccgcgggac catgcgcacg cccatccggc gcgatcggga
    10081 acggcaggca tgaaccgggc atagccgggt gttctcctgc ttccggcaac cgctcgaacg
    10141 gcgggccgac gggcgcgttt acctcgccga acgcgcacca cggatttacc ggctcgcacc
    10201 acccgcaccg caaccgaaga ctctctcccc aacccccccg aggaccttcg ccatgcgacg
    10261 tctagcccgc tacagcattg ccctgattct cgcgaccgcc gtctcgatcg ccggagtgca
    10321 ggtcgtccag gccgcctcgg gcgaggagaa cggcttcgtc tcccggatca actccgagcg
    10381 ctcgaagcgc gggatccaca cgctggtgtg ggccgaggac ctcgcccagg tcgcgcgccg
    10441 gcacagccag cgcatggcga gccaaaagaa cctgcaccac aacccgaacc ttcgcagcga
    10501 ggtggacggc tgggaggtcg tcggcgagaa cgtgggctac ggcccgggcg tcgaggacct
    10561 gcatcaggcc ttcatggact cccagagcca ccgcgccaac atcctcgacc ggagctacac
    10621 ccaggtcggc gtcggcaccg tctggcgcga cggcctgctg tgggtcaccc aggtcttccg
    10681 caagccggtc tcctcgtcga acacttcgaa cggatcgggt ccgtccgcga gcggctcgtc
    10741 gtcggcgccg cgcaaggcgt cgaagccgaa ggctgccgcc aagcccaagc ctgccgcgcc
    10801 ccggcccgta cccgctgccc ctgccgcacc gatccatccg ccttcgaccc cggccgccga
    10861 gctgacccgc cgcatgatcg agacgcttct cggcgggcag gagccggtgt ccggatcggt
    10921 cgaggccgcc gacttctccc tcgagccggc atcggtcggg agcggatcgg cgggcatgag
    10981 cctgcgctcg ttcgccgagc tggcgggccg cgtgaaccgc cgcctgctgg agcaggccgc
    11041 cggccgctga cgcgagcggt cccggacga
//
LOCUS       DATLFO010000107        10600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_11883, whole genome shotgun sequence.
ACCESSION   DATLFO010000107 DATLFO010000000
VERSION     DATLFO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11883"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..344)
                     /locus_tag="VM841_03865"
     CDS             complement(102..344)
                     /locus_tag="VM841_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89354.1"
                     /translation="MKAMKEVVSMTGAGPSDLHIDCETCVMRETDACRHCVVMAIVNP
                     PKGAIVFDAAEERAIRAMATAGLLPLVRYRPGSEAV"
     gene            487..630
                     /locus_tag="VM841_03870"
     CDS             487..630
                     /locus_tag="VM841_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89355.1"
                     /translation="MTVHAGDVLDDTKQVSCTSCGNRFEIEGRHAAPACPECGATEFE
                     ESA"
     gene            complement(655..882)
                     /locus_tag="VM841_03875"
     CDS             complement(655..882)
                     /locus_tag="VM841_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89356.1"
                     /translation="MTDDLRYRCNACGNLTRFDVVSRRRTRAFHHYTLGGDLTVEEEE
                     ILELDVEKVTCRWCGGSDQIERIPRTEEAAP"
     gene            968..2578
                     /locus_tag="VM841_03880"
     CDS             968..2578
                     /locus_tag="VM841_03880"
                     /inference="COORDINATES: protein motif:HMM:NF005905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HVL89357.1"
                     /translation="MKTLQRSFDDLGSALIETTFCIVDLETTGGAPGSSMITEIGAVK
                     VRGGEVLGEFRTLVNPGCEIPPMITVLTGITNAMVIGAPNIEEALPAFLEFCGDAVVV
                     AHNAAFDTRFLNYEAERLGYGRMENPVVCTVRLARRLVRDEVPNLRLETLAHALRART
                     PTHRALDDARATTDVLHALLELGGRWGVNHLEDLLWFQTAGGHPQAGKRKLADPLPGA
                     PGVYMFRAADGALLYVGKATDLRTRVRSYFSSDDRRRIGNMLRDMNRIEHHETALEIE
                     AAVLEGRLIRKHHPPYNRAGRGSTGKAWVRLTAERFPRIAIARTPGDGSIGPLSSAAA
                     TALREALEEAAPVRRCNDRIGAKTRFDPCALAQIGRCLAPCDGRVDPDAYAATLTPVR
                     TALDGDPTVALLAIDERLGRLTAETRFEEAAGVRDRAGSLVHALARRRAADALRAAGR
                     IVLDAGGVTVVIADGMLESAGGRALPAGADGHADETRLLWSWLTRRREIRLVSASGTF
                     AMPVQGGHAIAGWQKRLHKARRSRDRTT"
     gene            complement(2621..5002)
                     /locus_tag="VM841_03885"
     CDS             complement(2621..5002)
                     /locus_tag="VM841_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVL89358.1"
                     /translation="MKIRRARLVAAVIALVALAAGLPATTATASGYTADIRRTQFGIP
                     HIKAEGWGNLGFGVGYAFAQDNLCVMADTFLTVNAERSKYLGPNGTYRSEANGVVPTN
                     LESDYYYQWINESGVIEGLVDGTWPHAKPLSQPIVDLSEGWAAGYNAYLSDIGGAAQV
                     SDPACKGMPWVKPISVRDVYRRYYQLIMFASKGALLKFIVGAQPPLPGAPVPVATTAE
                     IDRLPSGENLGIGSNAWALGDEATDNGYGMLLGNPHFPWRGPERFYEFHLTWPGKVNV
                     MGGALFGSPLVHIGVTDHVAWSHTVSVPYRFTPYELKLVPGDPTAYFWNGGIKRMKTK
                     TVEVDVKQPDGSITRSPHTFYEAHFGPVINFQGALMPWTPAVAYAIDDANEDNFRALE
                     HFLDVNQAQSANDVYDALAEHVGIPWVNTIVADDRGSAFYADMSVVPHHTDEQLGRCT
                     TAVGEALKALARLPVLDARPDCMPGTDADAPVPGILGAGNLPSLFRRDYVANSNNSHW
                     LPHEHHPLEGYPYVTGAERAAITPRARLGLLMIREELANGGTFTLQEVQDAVFNNRQY
                     LGEIVGDDLIGACRQTPMIPIQEGNGVVMIDLTQACDVLAAWDRKANLDSKGTALFRE
                     FVNAAYAANTFWLDRFDADRPVDTPGRMNAASPEVRQALARAVRKLQNAGVALDAKLG
                     DIQRAKRGGLSIPVHGGSEHGMFNYVLSPFNAAEGGYTDTTYGSSFVITATWDAIGKP
                     RAEAILTYSLSTDPTSPHYADQTQLYSNKQWVPLYMDEASVLANTMTSIVVTG"
     gene            5132..5419
                     /locus_tag="VM841_03890"
     CDS             5132..5419
                     /locus_tag="VM841_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HVL89359.1"
                     /translation="MVTAIVLINSDVHKVPETAQALADLEHVSEVYSVSGEYDLVALV
                     RVAEHDQLADVVTAEIARIPSITATQTLIAFRAYSRHDLDSLFSIGFADAD"
     gene            5429..6109
                     /locus_tag="VM841_03895"
     CDS             5429..6109
                     /locus_tag="VM841_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVL89360.1"
                     /translation="MVAEAGVAGGFAGALAVPDLEALRAAGRSRRYPAGTTMLLEGDR
                     SANVILIEDGRVKISSFTEDGREIVLAVRVTGDLLGELSVIDGEPLSATASALEDVRA
                     TVVPADAFKQYLSTHAPASFALLEIVSRRLRDADRKRIEFGAYDSVGRVARRLLELAE
                     RFGEGDGDGAVRIALPLSQEEIAGWTGSSREAVSKALRALRERNVIDTARKVITVLDM
                     DALRKRAT"
     gene            complement(6127..9162)
                     /locus_tag="VM841_03900"
     CDS             complement(6127..9162)
                     /locus_tag="VM841_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL89361.1"
                     /translation="MAVCAACGGANPEGMRFCGHCAAPLDSTAPGRDDALRKMVSRQV
                     AERLAAGGELSDEWRLVTTLFADISGYVALGERLDPESYAEATGPIVALMTRVAERYD
                     AHVAKFAGDALLCMFGAPVAREDDADRALLAALEMRAELDALRPSLPEVARDLELHIG
                     VNTGRVVAAILGDAARLDYNVMGDAVNSAQRLESAAPGGEIYAGDATVRLVRDRFEFE
                     RVGELTVKGKREPIGAWRLIGRRRTAAPRASAPLVARDAEVAALAGVFSRLLGGRGAV
                     VCVTGEAGVGKSRLCADAASRVRPRGVRSLVARFPSAGSVAYGPYRDLVRSVAGIESG
                     DPAARAARALQRTCAALGLEAAEPYLARVAGLPGDTAVRDPEAVRRGVHEAFVALIDA
                     LVATSPLALTIEDFHWADESSAALTEEIAAMTAARPLALLLTARPEARAMLDAIAMRG
                     EASSRLPIALDPLDDAGVAMLVDNTLGGSAPAGLATVLRERAAGNPFFVEEIVRSLVE
                     AGTLRRAAGGWTVDGGWDAGGVPPTIEGVLAARLDRLPPEAVAVAQVAAVAGRRVRLR
                     LLHDVLGGANLDVAVAALVASGLVDRDGDDAEPSIAFHHALAAEVAYERLLRRRRTEL
                     HRMIADAAERLYGDGDDVVELLARHRYLGGGGEAAFELLVRAGERARRLYANGAALTH
                     FERALEVAPDARRPEIALAIADLCELRGDYARALAVYEEVRDATGDVRAWRGIASTMR
                     KRGDYDLAIEMLDAALVAGAADPAALRLEHGWTLSVAGRYREAIASLRTGLSAAPARR
                     DEVTAALLLELARAETVERDLVVALTHGEAARAIFAERGDERGLASSIRIVAAALHRL
                     GRLDEAAAALGDGLTLAERTGDVEERAACLINLGMVELERERIDDAIECDRRAIAEFE
                     RIGHASGRAIGYGNLAEKLLRAGDLGEAERWCERALEHARGIGHTPTIADATRTRALI
                     ALQRGDHAEARAAAEEAARVYEQMGVPAEAAEARRIGAG"
     gene            complement(9167..10198)
                     /gene="trpD"
                     /locus_tag="VM841_03905"
     CDS             complement(9167..10198)
                     /gene="trpD"
                     /locus_tag="VM841_03905"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HVL89362.1"
                     /translation="MSDFAWPDTLARLLRGEDLPQQEAAAAMRSIMDGRASPAQVAGF
                     LIGLRAKGETVDEVAGLAATMRELSNKVPVAGPLLDTCGTGGDRAGTVNISTMAALVV
                     AGAGGRVAKHGNRAASSRCGSADLLEGLGVVIDLSPEDVATCIDEAGIGFCFAPVFHP
                     AMRHAAVPRRELGVPTVFNFLGPLTNPAGAQRQTVGVSDPTMAPKMAEALRRLGAERA
                     MVFRGDDGLDELTTTTTSTVWDIAPDGVAERRFDPEVEHSIARATAADLAGADVETNV
                     KICLAVLEGEPGAVRDAVVLNAAAAMTVGGLAPSMDAALDAARESLDAGRARAAMERM
                     RDVSRRLKG"
     gene            10257..>10600
                     /locus_tag="VM841_03910"
     CDS             10257..>10600
                     /locus_tag="VM841_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HVL89363.1"
                     /translation="MRIAMGADDRNAVTDALIDGVKARGHEVVMVLGPAGGRDEQWAD
                     VGRGVALAVAKGEADQGIVLCHTGTGVSIAANKVPGVRAALCTDAEQARGARTWNDAN
                     VLAMSLRLTAEPL"
BASE COUNT         1564 a         3796 c         3713 g         1527 t
ORIGIN      
        1 gagcttgatc aacggacccc cagggaagcc ggaatacgcc cggcattctg gccggtccgt
       61 ccactcgccg caaggaaccc gggaacgaat cgggccgcgt gctacaccgc ttcactgccg
      121 ggccggtatc tcaccagagg aagcaagccg gccgtggcca tcgcccggat cgcccgctcc
      181 tccgccgcgt cgaagacgat ggcgcctttc ggcgggttga cgatcgccat cacgacgcag
      241 tgacggcatg cgtccgtctc gcgcatcacg caggtctcgc agtcgatgtg caggtccgat
      301 ggtccggcgc cggtcatgct caccacctcc ttcatggcct tcacggtaga ccaggggtac
      361 gacagcgaaa gaacgggatc gggcgaaacg atccttggcc ggatcgccgg cgaccgacgc
      421 ccgggctgat gattcggcct cgccgcattc gggaaagcgg gaagcgacga cggaaggagg
      481 ctcgagatga ccgtgcacgc cggagacgtc ctggacgaca ccaagcaggt gtcctgcacc
      541 tcgtgcggta accggttcga gatcgagggg cgccacgccg cgcccgcctg ccccgagtgc
      601 ggggcgaccg agttcgaaga gtccgcctga gaggacgaac cgtggggccg cgtcctaggg
      661 cgcggcctct tccgtgcgcg ggatgcgttc gatctgatcc gagccgccgc accagcggca
      721 cgtgaccttc tcgacgtcga gctcgagaat ctcttcctcc tcaacggtga ggtccccgcc
      781 gagcgtgtag tggtggaacg cacgcgtccg tcgccgggac accacgtcga agcgcgtcag
      841 gtttccgcac gcgttgcagc ggtagcgaag atcgtcggtc acggcggcgg cagtataccg
      901 agatcgctga tcccgaacag ctgttcggtt cgtgtcggcg ccccctcgta tagtcggatc
      961 cggcacgatg aagaccctgc agcgatcctt tgacgatctg ggctcggccc tgatcgagac
     1021 cacgttctgc atcgtggacc tcgagacgac cggcggcgcg ccgggctcgt cgatgatcac
     1081 cgagatcggc gcggtcaagg tccgcggcgg cgaggtgctc ggcgagttcc ggacactggt
     1141 caacccgggg tgcgagatcc cgccgatgat caccgtgctg accggcatca ccaacgcgat
     1201 ggtgatcggc gcgccgaaca tcgaggaggc gctgccggcg ttcctggagt tctgcggcga
     1261 cgcggtggtg gtcgcgcaca acgccgcctt cgatacccga ttcctgaact acgaggccga
     1321 gcggctcggc tacgggcgga tggagaaccc ggtcgtgtgc acggtacggc tggcgcggcg
     1381 gttggtgcgc gacgaggtcc cgaacctgcg gctcgagacg ctcgcgcacg cgctgcgggc
     1441 gcggacgccc acccatcgcg cgctcgacga cgcgcgcgcg accaccgacg tgctgcacgc
     1501 gctgctcgag ctgggtggcc gctggggcgt gaaccatctc gaagacctgc tgtggttcca
     1561 gaccgccggc gggcatccgc aggccggcaa gcgaaagctg gccgatcccc tgcccggcgc
     1621 gcccggcgtc tacatgttcc gcgccgccga cggcgcgctg ctgtacgtgg gcaaggccac
     1681 ggatctgcgc acgcgcgtgc gttcgtactt ctcgagcgac gaccgccgcc gcatcggaaa
     1741 catgctgcgg gacatgaacc ggatcgagca tcacgagacc gcgctcgaga tcgaggccgc
     1801 ggtcctggag ggccgcctga tccgcaagca tcatccgccg tacaaccgtg ccggccgcgg
     1861 ctcgaccggc aaagcctggg tgcggctgac ggccgagcgc ttcccgcgga tcgcgatcgc
     1921 gcgcacgccc ggcgacggct cgatcgggcc gctgtcgtcg gcggcggcga cggcactgcg
     1981 cgaggcgctc gaagaggccg cgccggtgcg gcggtgcaat gaccggatcg gcgccaagac
     2041 ccgcttcgac ccgtgcgcgc tcgcccagat cggccggtgc ctggcgccct gcgacggccg
     2101 cgtcgacccc gacgcctacg ccgcgacgct cacgccggtg cgaacggcgc tggacgggga
     2161 cccgaccgtg gcgctgctcg cgatcgacga gcggctcggg cggctgacgg ccgagacacg
     2221 cttcgaggag gcggccggcg tgcgcgaccg tgcgggctcg ctcgtacatg cgctggcacg
     2281 gcgccgcgcg gccgacgcgc tgcgcgccgc cggacggatc gtgctcgatg ccggcggcgt
     2341 gacggtcgtg atcgcggacg gaatgctgga atccgccggc gggcgcgccc tgccggccgg
     2401 cgccgacggc cacgccgacg aaacccgcct gctgtggtcg tggctgacgc gccgccgcga
     2461 gattcggctc gtgtcggcaa gcggcacgtt cgcgatgccc gtgcagggcg gccacgcgat
     2521 cgcgggctgg cagaagcggc tccacaaggc gcgccgcagc cgcgaccgca cgacatgacg
     2581 aggggccccc gccgggagcc cctcgatccg atgcgtctcg ctatccggtg acgacgatcg
     2641 acgtcatcgt gttcgcgagc accgatgcct cgtccatgta cagcggaacc cattgcttgt
     2701 tcgaatacag ctgcgtctga tccgcgtaat gcggtgacgt cggatccgtt gacaacgaat
     2761 aggtcaggat cgcttcggca cggggcttgc cgatcgcgtc ccacgtcgcg gtgatcacga
     2821 agctcgatcc gtacgtcgtg tcggtgtagc cgccctcggc cgcgttgaac gggctcagga
     2881 cgtagttgaa catcccgtgc tcggagccgc cgtggaccgg gatcgacagc ccgccacgct
     2941 ttgcccgctg aatgtcgccc agcttcgcgt ccaaggccac gccggcgttc tgcagcttgc
     3001 gcaccgcgcg ggcaagcgcc tgccgcacct cgggcgatgc cgcgttcatc ctgccgggcg
     3061 tatccaccgg ccggtccgcg tcgaaccggt ccagccagaa cgtgttcgcg gcataggcgg
     3121 cgttgacgaa ctcgcggaac agcgccgtcc ccttcgaatc caaattcgcc ttccggtccc
     3181 aggccgcgag cacgtcgcag gcctgcgtca gatcgatcat gacgacgccg tttccttcct
     3241 ggatcgggat catcggcgtc tgacggcagg cgccgatcag atcgtcgccg acgatctcac
     3301 ccaggtattg gcggttgttg aagaccgcgt cctgaacctc ttgcagcgtg aaggtgcctc
     3361 cgttcgcgag ctcctcgcgg atcatcagca gacccagccg ggcacgcggc gtgatcgcgg
     3421 cgcgctcggc gccggtcacg tacgggtagc cctccagcgg atgatgctcg tggggcagcc
     3481 agtggctgtt gttcgagttc gcgacgtagt cgcgacggaa caggctcgga aggttgccgg
     3541 cccccaagat gccgggcacg ggcgcgtcgg catcggttcc cggcatgcag tcgggccgcg
     3601 cgtcgagcac cggcagacgc gccagcgctt tgagcgcctc acccaccgcc gtcgtgcagc
     3661 gccccagctg ctcgtcggtg tgatgcggta ccaccgacat gtccgcgtag aacgccgatc
     3721 cgcggtcgtc ggcgacgatg gtgttcaccc acggaatgcc gacgtgctcg gccagcgcgt
     3781 cgtagacgtc gttcgcgctc tgtgcctggt tgacgtccaa gaagtgctcg agcgcgcgga
     3841 agttgtcctc attcgcgtcg tcgatcgcgt aggcgaccgc gggcgtccag ggcatcaacg
     3901 cgccctggaa attgatgacc ggaccgaagt gcgcctcgta gaaggtgtgc ggcgaccggg
     3961 tgatcgagcc gtcgggctgc ttgacgtcca cctcgaccgt cttggtcttc atccgtttga
     4021 tgccgccgtt ccagaagtag gcggtcggat cgcccggcac cagcttcagc tcgtagggcg
     4081 tgaaccggta gggcacggac acggtgtggc tccacgcgac gtgatccgtg acgccgatgt
     4141 gcaccagcgg gctgccgaac agtgcgccgc ccatcacgtt gaccttgccg ggccaggtca
     4201 ggtggaattc gtagaaacgt tccgggccgc gccacgggaa gtgggggttg cccagcagca
     4261 tgccgtagcc gttatcggtc gcctcgtcgc caagcgccca cgcgttcgat ccgatgccca
     4321 ggttctcgcc gctcggaagc cggtcgatct cggcggtcgt tgcgaccggg accggcgcac
     4381 cgggcagcgg cggctgcgcg ccgacgataa atttcagcag cgcgcccttc gaggcgaaca
     4441 tgatcagctg gtagtagcgg cgatagacgt cgcgcacgct gatgggcttg acccagggca
     4501 tgcccttgca ggccgggtcg ctgacctgtg ccgcgccgcc gatgtcgctc aggtaggcgt
     4561 tatagccggc cgcccagcct tcgctcagat ccacgatcgg ttgcgacaat ggcttggcgt
     4621 gcggccaggt gccgtccacg agcccttcga tcacgccgga ctcgttgatc cactggtagt
     4681 agtagtcgct ttcgagattg gtcggcacga cgccgttcgc ctcggagcgg taggtgccgt
     4741 tcggccccag atacttggac cgctcggcgt tgacggtcag gaacgtgtcg gccatcacgc
     4801 agaggttgtc ctgtgcgaac gcgtagccga cgccgaaccc taggttcccc caaccttccg
     4861 ccttgatgtg cgggatcccg aactgggtgc ggcggatatc ggcggtgtag cccgaggcgg
     4921 ttgccgtagt ggccggcaat ccggcggcaa gcgcgaccaa ggcgatgacg gcggcgacga
     4981 gccgcgcgcg gcgtatcttc acgatcaacc tcccgttttg atcgatgagc ttgctgctac
     5041 cttcgccgcc gatcgaaaca atccttcatg ccgcgaacat cgaggcccct ttgcagaagt
     5101 ggatcgggtc cggggtagac tcagcgccgc catggtgacc gcgatcgttt tgatcaacag
     5161 cgacgtgcac aaagtgccgg agaccgcgca ggccttggcc gacctcgagc acgtctccga
     5221 ggtgtactcg gtctccggcg agtacgacct ggtcgcgctg gtgcgcgtcg ccgagcacga
     5281 ccagctcgcc gacgtcgtca cggccgagat cgctcgtatc ccgtcgatca cagccacgca
     5341 gacgctgatc gcgttccgcg cgtattcgcg ccacgatctg gactcgctgt tctcgatcgg
     5401 cttcgccgac gcggactgag cggagggcgt ggtggccgaa gcgggcgttg cgggcgggtt
     5461 cgcgggggcg ctcgccgttc ccgatctgga agcgctgcgc gccgcgggac ggtcgcgccg
     5521 ctaccccgcc ggcaccacca tgctgctcga aggcgaccgc agcgccaacg tgatcctgat
     5581 cgaggacggc cgggtgaaga tctcctcgtt caccgaggac ggccgcgaga tcgtgctcgc
     5641 ggtgcgtgtc accggcgatc tgctgggcga gctgtcggtg atcgacggcg agccgctgtc
     5701 ggcgacggca tcggcgctgg aggacgtgcg cgcgacggtc gtgcctgccg acgcgttcaa
     5761 gcagtatctt tcgacccacg cgcccgcctc gttcgcgttg ctcgagatcg tgtcgcggcg
     5821 cctgcgcgac gccgaccgca aacgcatcga gttcggcgcc tacgactcgg tcggccgtgt
     5881 cgcgcgccgg ctgcttgagc tcgccgagcg cttcggcgaa ggagacggcg acggggccgt
     5941 gcgcatcgcg ctgccgctgt cgcaagagga gatcgccggg tggaccggct ccagccgtga
     6001 ggccgtgagc aaggcgctgc gcgcgctccg cgaacgcaac gtgatcgaca ccgcacgcaa
     6061 ggtgatcacc gtgttggaca tggacgcgct ccgcaagcgc gccacctaga cggctctttc
     6121 gctgcgtcag cccgcgccga tgcggcgggc ctcggccgct tcggccggga cgcccatctg
     6181 ctcgtagacc cgcgccgcct cctcggccgc cgcgcgcgct tcggcgtggt cgccgcgctg
     6241 caacgcaatc agggcgcggg tgcgggttgc atcggcgatc gtgggcgtgt gcccgatccc
     6301 gcgggcatgc tcgagcgctc gctcgcacca ccgctcggct tcgccgaggt cacccgcgcg
     6361 cagcagcttt tcggcgagat tgccgtagcc gatcgcgcgg ccgctcgcgt gaccgatgcg
     6421 ctcgaactcg gcgatggcgc gacgatcgca ttcgatcgcg tcgtcgatcc gctcgcgctc
     6481 gagctcgacc atcccgaggt tgatcaggca ggcggcgcgc tcttcgacgt cgccggtccg
     6541 ttcggccagc gtcaagccgt cgccgagcgc ggccgcggcc tcgtcgagcc ggccgaggcg
     6601 gtgcagcgcc gcggccacga tccgaatgct cgacgcaagg ccgcgctcgt cgccccgctc
     6661 ggcgaagatc gcgcgggcgg cctcgccgtg ggtcagcgcg accacgagat cgcgctcgac
     6721 ggtctcggcg cgggcgagct cgagcagcag cgcggcggtt acctcgtcgc ggcgggcggg
     6781 cgcggcggac agccccgtgc gcagcgaggc gatcgcctcg cggtagcgtc ccgcgaccga
     6841 cagcgtccat ccgtgctcga gccgcagcgc cgcgggatcg gcggcgccgg ccaccagcgc
     6901 cgcgtcgagc atctcgatcg caaggtcgta atcgccacgc tttcgcatcg tggacgcgat
     6961 cccgcgccag gcgcgaacat ctcccgttgc gtcccgcacc tcctcgtaca ccgcaagcgc
     7021 gcgcgcatag tcgccgcgca gctcgcacag gtccgcgatc gcgagcgcga tctcggggcg
     7081 acgcgcatcc ggcgcgacct cgagcgcccg ctcgaagtgc gtcagcgccg caccgttcgc
     7141 gtacaggcgc cgcgcgcgct cccccgcccg cacgagcagc tcgaaggcgg cctccccgcc
     7201 gccgccgaga taccggtggc gggcgagcag ctcgaccacg tcgtcgccat cgccgtacag
     7261 gcgctcggcc gcatcggcga tcatgcggtg aagctcggtg cggcggcgac gaagcagccg
     7321 ctcgtaggcg acctcggccg cgagcgcgtg atggaacgcg atggacggct cggcgtcgtc
     7381 gccgtcacgg tccacgagcc cggatgcgac gagcgccgcg accgcgacgt cgaggttcgc
     7441 accgccgagc acatcatgga gcagccgcag ccgcacgcgg cgccccgcga cggccgcaac
     7501 ctgcgcgacc gccaccgcct cgggcggaag ccggtccagg cgcgcggcga gcacgccctc
     7561 gatggtgggc ggcacgccgc ccgcatccca tccaccgtct accgtccagc cgccggccgc
     7621 gcggcgcagc gtgccggctt cgacgagcga gcgcacgatc tcctcgacga agaaggggtt
     7681 gccggccgcg cgctcccgca gcaccgtcgc gagccccgcc ggtgcggacc ccccgagcgt
     7741 gttgtccacg agcatcgcca cgcccgcgtc gtcgagcgga tcgagcgcga tcggcagacg
     7801 cgacgacgcc tcgccgcgca tcgcgatcgc atcgagcatc gcacgcgcct cgggccgcgc
     7861 ggtcagcagc agcgccagcg gacgggccgc ggtcatcgcc gcaatctcct cggtgagcgc
     7921 ggccgaggat tcgtccgccc agtgaaaatc ctcgatcgtg agcgcgagcg gcgaggtcgc
     7981 tacgagcgcg tcgatcaacg cgacgaacgc ctcgtgcacg ccgcggcgaa cagcctcggg
     8041 atcgcgcacg gccgtgtcgc cgggcagccc cgccacacgc gcgaggtagg gctcggccgc
     8101 ttccaggccg agcgcagcgc aggtgcgttg caacgcacgc gccgctcgcg cggccgggtc
     8161 gcccgactcg atgcccgcga ccgagcgcac cagatcgcga tacggcccgt aggcgaccga
     8221 gccggcgctc gggaagcgcg ccaccagcga ccggacgccg cggggacgca cgcgcgaggc
     8281 cgcgtcggcg cacagccgcg acttgcccac gcctgcctcg cccgtgacgc acaccacggc
     8341 gccgcggccg ccgagcagcc gcgagaacac cccggcgagc gccgcaacct ccgcgtcgcg
     8401 cgccaccagc ggcgcgctcg cgcgcggcgc ggcggtgcgg cggcggccga tcagacgcca
     8461 cgcgccgatc ggctcgcgct tgcctttgac ggtcagctcg ccgacgcgtt cgaactcgaa
     8521 gcggtcgcgc acgagccgca cggtcgcgtc gccggcgtag atctcgccgc cgggcgcggc
     8581 cgactcgagc cgctgcgccg agttcaccgc gtcccccatc acgttgtagt cgagccgcgc
     8641 cgcgtcgccc aggatcgccg cgacgacgcg gccggtgttc acgccgatgt gcagctcgag
     8701 gtcgcgcgcg acctcgggta gcgacgggcg cagcgcgtcg agctcggctc gcatctcgag
     8761 cgccgcgagc agggcgcgat ccgcgtcgtc ctcgcgggcg acgggcgcgc cgaacatgca
     8821 cagcagcgcg tcgccggcga acttggcgac gtgcgcgtcg tagcgttcgg cgacgcgggt
     8881 catcagcgcg acgatcgggc cggtggcctc cgcgtaggac tcggggtcga gccgctcgcc
     8941 cagcgcgacg tagccgctga tgtcggcaaa gagcgtggtg acgagccgcc actcgtcgct
     9001 gagctcgccg ccggcggcga ggcgctcggc cacttgacgc gaaaccatct tgcgcagcgc
     9061 gtcgtcgcgg ccgggcgcgg tcgaatccag cggcgccgcg cagtgaccgc agaagcgcat
     9121 cccctcggga ttcgcgcctc cgcaggcggc gcagacggcc acggtcctag cctttcagcc
     9181 gccgtgatac gtcgcgcatc cgctccatcg ccgcgcgcgc acggccggcg tcgagcgact
     9241 cgcgcgccgc atccagcgcc gcatccatcg acggagcgag cccgccgacg gtcatcgcgg
     9301 ccgcggcgtt gagcacaacc gcgtcgcgca ccgcacccgg ctccccctcc agcacggcca
     9361 ggcagatctt cacgttggtc tcgacatcgg ccccggcgag atccgccgcg gtcgcgcgcg
     9421 cgatcgaatg ctcgacctcg ggatcgaagc ggcgctcggc gacgccgtcc ggcgcgatgt
     9481 cccacaccgt cgaggtggtc gtggtggtca gttcgtcgag cccgtcgtcg ccgcggaaca
     9541 ccatcgcgcg ctccgcgccg agccgccgca gcgcctcggc catcttcggc gccatcgtcg
     9601 gatcgctcac cccgaccgtc tgccgctggg cgccggcagg gttggtcagc gggccgagaa
     9661 agttgaacac ggtcggcacg cccagctcgc ggcgcggcac ggccgcgtga cgcatcgccg
     9721 gatggaacac cggcgcgaag cagaagccga tcccggcctc gtcgatgcac gtcgccacgt
     9781 cctcgggcga aagatcgatc accacgccga gcccttccag cagatccgcg ctgccgcagc
     9841 gcgaggaggc cgcgcgattg ccgtgcttgg cgacccggcc gcccgcgccc gcaacgacca
     9901 gcgccgccat cgtcgagatg ttgaccgtgc ctgcgcggtc gccgccggtg ccgcacgtgt
     9961 cgagaagcgg accggcgacc ggcaccttgt tggacagctc gcgcattgtg gcggccagcc
    10021 cggcgacctc gtcaacggtc tcgcccttgg cgcgcaggcc gatcaagaat cccgcgacct
    10081 gcgcgggcga ggcccggccg tccatgatcg aacgcatcgc ggcggcggct tcctgttgcg
    10141 gcagatcctc gccgcgaagc agccgggcaa gcgtgtctgg ccacgcgaaa tcgctcatca
    10201 cgcgcgaagt atcgcatgtt cgcgcgggcg ggtgtccgga gtatcctcga agcacgatgc
    10261 gaatcgcaat gggagccgac gaccgcaacg cggtcaccga cgcgctgatc gacggcgtca
    10321 aggcccgcgg acacgaggtc gtgatggtcc tgggaccggc cggcggccgc gacgagcagt
    10381 gggcggacgt gggccgcggc gtcgccttgg cggtggccaa gggcgaggcc gaccagggca
    10441 tcgtgctgtg ccataccggc acgggcgtgt cgatcgcggc gaacaaggtg ccgggcgttc
    10501 gcgccgcttt gtgcaccgac gcggagcagg cgcgcggcgc gcgcacatgg aacgacgcga
    10561 atgtgctcgc gatgtcgctt cgcctgacgg ccgagccgct
//
LOCUS       DATLFO010000108         9807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_111734, whole genome shotgun sequence.
ACCESSION   DATLFO010000108 DATLFO010000000
VERSION     DATLFO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111734"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..424
                     /locus_tag="VM841_03915"
                     /pseudo
     CDS             1..424
                     /locus_tag="VM841_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
     gene            435..863
                     /gene="rplQ"
                     /locus_tag="VM841_03920"
     CDS             435..863
                     /gene="rplQ"
                     /locus_tag="VM841_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVL89364.1"
                     /translation="MPKPKKGPRLGSGPAHQKLILSGMASSIIRDGRVKTTEARAKAV
                     RPVVERMITHAKKGDLAARRQVLRTIRDRDIVHHLFAEVAPQYASRPGGYTRIVKLGP
                     RKGDAAPMAMIELVEGEQIVPAETEEAPKRRSRRKKSDAE"
     gene            930..1691
                     /gene="truA"
                     /locus_tag="VM841_03925"
     CDS             930..1691
                     /gene="truA"
                     /locus_tag="VM841_03925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVL89365.1"
                     /translation="MEPVRVAMLLAYDGAGFRGFARQRERRTVQGEIERTVSLVARRE
                     ILTTGAGRTDAGVHAAGQVMTFDAPASLDLTELAKRINKMLAPEISIKAVVAAPPGFD
                     ARFSARRRIYEYRIYGGPAPDPFLDRFALWEPRPLDVGAMNDAARHLVGEHDFASFCR
                     ANAKSLVRRIRIVRVRAARDSRTTIRVEGDSFCHQMVRSITGCLLAVGFGDRMPEWVG
                     NVLRAKNRQAAADVAPARGLTLMRVVYRPDPFASR"
     gene            1925..3667
                     /locus_tag="VM841_03930"
     CDS             1925..3667
                     /locus_tag="VM841_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89366.1"
                     /translation="MRPARLLVLALLTAILAVPTAPTAHEGNYPILLVHGWTSSGATF
                     DEMIPKLTAQGLTVLDCDSATSGIQALSYAPTASSQHISYVAGKILQPKIDSCLTANG
                     YSTSQKIDVVAHSMGGLSSRFLVEKGGADVDYWSSTTGWYGDGVADVSTKWKSQIDDL
                     IMLGTPNHGTILAWFPSTLPVFGAWSSTGADMRPGSKFLSLMGTIEPVGEYYSCGGGD
                     PWYLQLYQSDFDNDGVTHGFDGVVPAEAPFMTGCDNVLFSSNHSGLRTDDAPLDWVIQ
                     TLGYTSTQTGTGGANLAGQAVVKLEYFDVVKDHDGGTTDEFRFEIAVDTNGNNDGYAV
                     YQTLSYNRDAPFTQNWGNSGPATSVVINLPGTAPRMDVKVRVWEDDTGWGGGQECVST
                     HSFTDITQSEDIDGLENITATAADCKGGTNTLKISVYGVTADTGQTRLVTFGFDKAWI
                     ENDHDPTGNGEVQFTATAGRQGYTSTFYRGQPGNGTHYSRTNNTWVNIGTDANNKGTV
                     EPETIWKIRMLNSATYRFDVTYWEDDGGWSSRDGGNMYFMEGNVGTQAAGRVNYTGTA
                     HTDWDGYFYIVTEG"
     gene            3784..4266
                     /locus_tag="VM841_03935"
     CDS             3784..4266
                     /locus_tag="VM841_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVL89367.1"
                     /translation="MSAVRRLAALVSLVSAFGIAAPAGAVHFYRGPGSGCTPADGARS
                     DGGQGEIAATVNVLHNTFADAATALPVTRINAGEAIRWTWNSVHCHSVQAAGFYSGFH
                     YPAPEPGSPRALPGVFDYPMLEENPTFSFTVTFDTPGTYSYVCEHHAAIGMAGLVVVE
                     "
     gene            4370..4807
                     /gene="rplM"
                     /locus_tag="VM841_03940"
     CDS             4370..4807
                     /gene="rplM"
                     /locus_tag="VM841_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVL89368.1"
                     /translation="MKTFTPKASDVQRAWWVVDADGQVLGRLAAEIATILRGKHKPVF
                     APHMDMGDYVVVINAGKIRLTGDKLNQKMYRRHSGFPGGLRETKYGDLLAQKPEIAIE
                     KAVKGMLPHNRLGRAMLKKLKVYGGSEHPHASQDPKPLSVNGG"
     gene            4830..5210
                     /gene="rpsI"
                     /locus_tag="VM841_03945"
     CDS             4830..5210
                     /gene="rpsI"
                     /locus_tag="VM841_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HVL89369.1"
                     /translation="MTRATGRRKESVARVRLVPGTGQFQINGRTLEDYFPRPVLRMVV
                     TEPLRITSTSERFDVLATLEGGGMSGQAGALRHGIARALTIVDPTLRTDLKRRGLLTR
                     DARAKERRKYGLKKARKAPQYSKR"
     gene            5239..6564
                     /gene="glmM"
                     /locus_tag="VM841_03950"
     CDS             5239..6564
                     /gene="glmM"
                     /locus_tag="VM841_03950"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HVL89370.1"
                     /translation="MPRLFGTDGIRGHYGTDFDAAFVRRLGRAAAEVLGRGHPSPRIL
                     IGRDTRASGPEIERALASGIAAANGIPVLAGVIPTAAVAHIVHAGGFEAGAVISASHN
                     PAQDNGIKFFGDNGMKLADAIEDEVEAAMGRNGGDANDGEESWETLPDAAERYIEFLL
                     EDTPSLAGMKVVVDAANGAASGIAADTYRRAGATVITLYDEPDGRNINDGCGAVHPEA
                     LQAAVLERGAQAGIAHDGDADRLVAVDERGALVDGDQILAICALDARRRGELPQDAVA
                     TTVMANLGFRQAMAAEGIRVAETPVGDRYVLERMLAEGIVMGGEQSGHLIFLDRHTTG
                     CGILSALRLLGIMAGTAMPLSRLAASAPRLPQVLLNVRVAQRGGLDDATDVWDEVRRV
                     TAELGEDGRVLVRASGTEPVVRVMVESSDQDRAAAAAERIAKAVTDALG"
     gene            6604..8424
                     /gene="glmS"
                     /locus_tag="VM841_03955"
     CDS             6604..8424
                     /gene="glmS"
                     /locus_tag="VM841_03955"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HVL89371.1"
                     /translation="MCGIIGYVGEQQALPILMDALRRLEYRGYDSAGVAVLDGGLQVV
                     RKAGKLDELDKELAPSGLHGTTGIGHTRWATHGPPTDENAHPHSDCDGRIAVIHNGIL
                     ENFLELRAELEAEGHTLGSDTDTEAIAHLIEAVYDGDIVAATRKALTRLSGAFSIAVV
                     AAGEPDVVVAAKRTSPLIVGRGDHGTLLASDPTALIPYTRDVVHLLDDQVAVIRREGF
                     EITTLDGAPAEGTAIHIDWDLEAAEKGGYDDFMLKEIYEQPGAVADSLRGRLRGGRLH
                     LDEVRLSEEELRGIDKVFVVACGSAYNAGLVAKYAIEHWCRVPVEIDISSEFRYRDPV
                     LGKDTLAVAVSQSGETIDTLEAVRHARRQRAKVIAVSNTVGSSIPREADGVLYTQAGP
                     EVGVAATKTFITQMVVLKLLALYLAQVRGTIYPEEIARIVEDLESLEGKVETVLGSRQ
                     QVRGIAEEIHQAADVLFLGRHVGFPLALEGALKLKEISYIHAEGYAAGELKHGPIALI
                     SEGTPVVVVATQCHVYAKVIANIQEVKARGARVIAVATEGDTEVAKHADHVITVPSTH
                     ELLTPELVVVPLQFLSYEIAKMRGCDVDKPRNLAKSVTVE"
     gene            8428..>9807
                     /locus_tag="VM841_03960"
     CDS             8428..>9807
                     /locus_tag="VM841_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVL89372.1"
                     /translation="MLDALTVEDVRRQDAECEARGIPVERLMERAGFGVARAARAMLG
                     YSYGMRVVVVCGKGNNAGDGLVAGRWLAHWGAHVVAVMALGRELRGAAGANLARFPGP
                     VVDAGALDRELARADLVIDALFGVGLSRAPGGEAARAIDAINGAGAPVISIDVPSGMD
                     ADTGDAPGAVVRADGIVTLGGLKPGLLFHADVADWVEIADIGVPRDLRIGSALVPEAS
                     DVASLLPLRAPGGHKRDSGTVLVVAGSRAMPGAAALTAGACVHAGAGLTTLAATERVT
                     AVALSRVPEMTTIVLPDSPEGALDDKGAEIVLERADQFSAIAIGPGLSRHPATAEAVR
                     RILLSTDVPAVVDADAIVAFAGGPGALRERRGFTVLTPHGGEAAALLGAEAADLRADR
                     IAAARRIARETGQVALLKGPGTVVCGEDGPVYLNATGNRGLAQGGTGDVLTGVIAALL
                     AQGGDAPDET"
BASE COUNT         1587 a         3382 c         3426 g         1412 t
ORIGIN      
        1 catctgctcg acccgggtcg cctcgaccga gtacgagacc cgggtcgagc agatgaccga
       61 ccgcgacaag ctgatcctcg acgtcgagac gaacggcgcc atctcgcccc gcgaggcggt
      121 ctcgtcgggc ggcaccacgc tggtcgagct ggcagggttg ttcggtgagc tctccgagag
      181 ccccggcctc tcgctcgggc ccgccgaggg cgaggcccag ccgagcggtg agctcgccct
      241 gccggtcgag gagctcacgc tctcggtccg ctcgtacaac tgcctcaagc gcgagggcgt
      301 cgccaccgtc ggcgatctga tccagcgcac cgagcaggat ctgctcgaca taaggaattt
      361 cggtcagaag tccatcgacg aggtcaagat gaagctcgcc gcgatgggca tgggattgcg
      421 agagggctag agccgtgccg aagccgaaga agggtccgcg cctcgggtcg gggcccgcgc
      481 accagaagct gatcctgagc gggatggcgt catcgatcat ccgcgacggt cgcgtcaaga
      541 cgacggaagc acgcgccaag gccgtacgtc cggtcgtcga gcgcatgatc acgcacgcga
      601 agaagggcga tctggcggcg cgccgtcagg tgttgcgcac gatccgcgac cgcgacatcg
      661 tgcatcacct cttcgccgag gtcgcgccgc agtacgcgtc acgtccgggc ggctacacgc
      721 gcatcgtcaa gctcggcccc cgcaagggtg acgccgcgcc gatggcgatg atcgagctgg
      781 tcgagggcga gcagatcgtt cccgccgaga ccgaagaagc gcccaagcgc cggagccgcc
      841 gcaagaagag cgacgcggag tagcgcatcg ggagttcatg gaaggggcct tcgggcccct
      901 tcttcacgtc tgcggctatc ctcgccggta tggaaccggt gcgggtggcg atgctgctgg
      961 cctacgacgg ggccggcttc cgcggcttcg cgcggcagcg tgagcgacgc accgtccagg
     1021 gcgagatcga gcgtaccgtc tcgctggtcg cgcggcgcga gatcctgacc accggcgccg
     1081 gacgcaccga cgcgggcgtt cacgccgccg ggcaggtgat gaccttcgac gcgccggcgt
     1141 cgctggacct gaccgagctc gccaagcgga tcaacaagat gctggcgccc gagatctcga
     1201 tcaaggccgt cgtcgccgcg ccgccgggat tcgacgcgcg cttctcggca aggcgcagaa
     1261 tctacgagta ccgcatctac gggggaccgg cccctgatcc gttcctggac cggttcgccc
     1321 tgtgggagcc gaggccgctc gacgtcgggg cgatgaacga cgccgcacgg catctggtcg
     1381 gcgagcacga cttcgcctcg ttctgccgcg ccaacgctaa gtcactggtg cgccggatcc
     1441 gcatcgttcg cgtgcgggcc gcccgcgatt cgcggaccac gatccgcgtc gaaggcgact
     1501 cgttctgtca ccagatggtg cgcagcatca cgggctgcct gctggccgtg ggcttcgggg
     1561 accgcatgcc ggaatgggtg ggcaacgtgt tgcgggcgaa gaaccggcag gcggccgccg
     1621 acgtcgcccc tgcgcggggc ttgacgctga tgcgggtcgt ctaccggccg gacccgttcg
     1681 cgtcgcgcta ggcacggtcc ggaagggcct gcccattcgc cgcggaccgc cgggcaggaa
     1741 agagcacgct gcgcgacgaa tggcgggacg ctgtccggtg cgcctgattg ctcctcgaac
     1801 ctctccggca gaggccgccc ggccctggac aaaggggaat ttctgctcgt aaattcccgg
     1861 cgggtaaccc gacgttggtt gaccgtcact cccgtggacg ggcgccgggt ttggagggaa
     1921 ccgcatgcgg ccagcacgtc tgctcgtgct cgcgctcctc accgcgatcc tcgccgtgcc
     1981 gacggcgccg accgcgcacg agggcaatta tccgatcctg ttggttcacg gctggacgtc
     2041 gagcggcgcc acgttcgacg agatgatccc caagctcacc gcgcagggcc tgaccgtcct
     2101 ggattgcgac agcgccacgt ccggcatcca ggcgctgtcg tacgcaccca cggcgtcctc
     2161 gcagcacatc tcctacgtcg cgggcaagat cctgcagccc aagatcgact cgtgcctgac
     2221 ggccaacggc tattcgactt cgcagaagat cgacgtcgtc gcgcactcga tgggcggcct
     2281 gtcgtcgcgc tttttggtcg agaagggcgg tgcggacgtc gattactgga gctcgacgac
     2341 cggctggtac ggagacggcg tcgccgatgt cagcacgaag tggaagagcc agatcgacga
     2401 cctgatcatg ctcggcaccc cgaaccacgg aaccatcctc gcctggtttc cctcgacgct
     2461 gccggtcttc ggggcgtggt cctcgaccgg cgccgacatg cgccccgggt cgaagttcct
     2521 ttcgctgatg ggaacgatcg agccggtggg tgagtactac tcgtgcggtg gcggggaccc
     2581 gtggtacctg cagctgtatc agagcgactt cgacaacgac ggcgtcacgc acggcttcga
     2641 cggcgtcgtc ccggccgagg cgccgttcat gacgggctgc gacaacgtgc tgttctcgtc
     2701 gaaccattcg gggcttcgca ccgacgacgc gccgctcgac tgggttatcc aaacgctcgg
     2761 ctacacctcg acccagaccg gaaccggcgg cgcgaacctt gccggtcaag ccgtcgtcaa
     2821 gctcgaatac ttcgacgtcg tcaaagatca cgacggtgga acgaccgacg agttccggtt
     2881 cgagatcgcg gtcgacacca acggcaacaa cgatggatac gccgtgtacc agaccttgtc
     2941 ctacaaccgc gatgccccgt tcacgcagaa ctgggggaac agcgggccgg cgacctcggt
     3001 cgtgatcaat ctgccgggca ccgcgccgcg catggacgtg aaggtccgcg tctgggagga
     3061 cgacaccggc tggggtggcg gacaggagtg cgtctcgacg cactccttca ccgacatcac
     3121 ccagtccgag gacatcgacg ggctcgagaa catcaccgcg accgccgcgg actgcaaggg
     3181 cgggacgaac acgctgaaga tctcggtgta cggcgttacc gccgacaccg gccaaacgcg
     3241 tctggtcacg ttcggcttcg acaaggcgtg gatcgagaac gaccacgacc cgaccggcaa
     3301 cggcgaggtc cagttcaccg caaccgcagg ccgtcaggga tacacctcga cgttctatcg
     3361 cgggcaaccc ggcaacggca cccattactc acgcacgaac aacacgtggg tcaacatcgg
     3421 aaccgacgcc aacaacaagg gcacggtgga gccggagacg atctggaaga tccgcatgct
     3481 gaactccgcg acctatcgct tcgacgtcac ctattgggag gacgacggcg gctggagctc
     3541 gagggacggc ggaaacatgt acttcatgga aggcaacgtc gggacccaag cggcaggccg
     3601 cgtgaactac accggcaccg cgcacaccga ctgggacggc tacttctaca tcgtcaccga
     3661 aggctaggtc gcggtcgtcg tggggcccgg gaccgatccc gggccccacc catgccggca
     3721 ggattgcggc cggcgtccgc agaagaagat gaggacgacc gttccgaaaa caagggggat
     3781 cctatgagcg ccgtccgccg cctcgccgcg ctggtctcgc tggtctcggc tttcggcatc
     3841 gccgcgcccg ccggcgcggt ccacttctac cgcgggcccg ggagcggatg cactccggcc
     3901 gacggcgcac gatccgacgg ggggcagggc gagatcgcgg cgaccgtgaa cgtcctgcac
     3961 aacacgttcg ccgacgcggc gaccgcgctg ccggtcacgc ggatcaacgc cggagaggcg
     4021 attcggtgga cctggaacag cgtccactgc cactcggtcc aggccgccgg cttctacagc
     4081 ggcttccact atcccgcgcc cgagccggga tcgccgcgcg cgctgccggg cgtcttcgac
     4141 tacccgatgc tcgaagagaa tcccaccttc tcgttcaccg tgacgttcga cacgccgggc
     4201 acgtactcgt acgtgtgcga gcaccatgcc gcgatcggca tggcagggct cgtcgtcgtc
     4261 gaatagggca ttccggtcgt tcccggctgc ccgggaggcc gggttgaccc gctccgaggg
     4321 ggccggtacc attgcccggt cccccaccca cgtgggggct tcgcacgcca tgaagacatt
     4381 tactccgaag gcttccgatg ttcagcgcgc ctggtgggtc gtcgatgcag acggccaggt
     4441 gcttggtcgt cttgccgcag agatcgccac gattctgcgc ggcaagcaca agccggtgtt
     4501 cgcgccgcac atggacatgg gcgactacgt cgtcgtcatc aatgcgggca agatccggct
     4561 gaccggagac aagctcaacc aaaagatgta ccggcggcac tcgggctttc ccggcgggct
     4621 ccgcgagacc aagtacgggg acctgctcgc ccagaagccg gagatcgcga tcgagaaggc
     4681 cgtcaagggc atgctcccgc acaaccgtct cggccgcgcg atgctcaaga agctcaaggt
     4741 gtacggcggc tcggagcatc ctcacgcttc gcaggatccg aagccgctct cggtgaacgg
     4801 aggctagggt ggcgacgacg gccggagaga tgacccgcgc gacgggacgc cgcaaggagt
     4861 ccgtggctcg cgtccgcctg gttcccggaa cgggacagtt ccagatcaac ggccgcacgc
     4921 tcgaggacta cttccctcgc cccgtgctcc ggatggtcgt gaccgagccg ttgcgcatca
     4981 cctcgacgag cgagcggttc gacgttcttg ccaccctcga aggcggcggg atgagcgggc
     5041 aggccggcgc gctgcgccac ggcatcgccc gcgcgctcac catcgtcgat ccgacgctgc
     5101 gcaccgacct gaagcgccgc ggcctgctga cgcgggacgc gcgcgccaag gaacgccgca
     5161 agtacggcct caagaaggcc cgcaaggcgc cgcagtactc gaagcgctag ccgcctcggg
     5221 tccgtttcgg cgccttccat gccccgcctc ttcggaaccg acgggattcg cggccactac
     5281 ggcacggact tcgacgccgc cttcgtccgt cgtctcgggc gggctgccgc cgaggtcctc
     5341 ggacgcggcc atccgtcgcc ccgcatcttg atcggccgtg acacccgtgc ctccgggccc
     5401 gagatcgagc gcgcgctcgc gtcggggatc gcggccgcca acggcatccc ggtcctggcc
     5461 ggcgtcatcc cgaccgccgc ggtcgcgcac atcgtgcatg ccggcggctt cgaggcgggt
     5521 gcggtcatca gcgcctcgca caatcccgcg caggacaacg gcatcaagtt cttcggcgac
     5581 aacggcatga agctcgccga cgcgatcgag gacgaggtcg aggccgcgat gggccgcaac
     5641 ggcggcgacg cgaacgacgg tgaggagtcc tgggagacgc ttcccgacgc ggccgagcgc
     5701 tacatcgagt tcttgctcga agacacgccg tcgcttgcgg gcatgaaggt cgtggtcgat
     5761 gccgccaacg gcgcggcgag cggcatcgcc gccgacacgt atcgccgggc gggtgcgacc
     5821 gtgatcacgc tctacgacga gcccgacggc cgcaacatca acgacggctg cggcgcggtt
     5881 catcccgagg cgctgcaggc cgccgtgctc gagcgcggcg cccaggccgg catcgcgcac
     5941 gacggcgacg ccgaccgctt ggtcgccgtg gacgagcggg gcgcgctggt agacggggac
     6001 cagatccttg cgatctgcgc gctggacgcg cgccgccgcg gggagctgcc gcaggacgcg
     6061 gtggcgacca cggtgatggc gaacctgggt ttccggcagg ccatggcggc cgaaggcatc
     6121 cgggtcgccg agacgccggt cggagaccga tacgtcctgg aacgcatgct cgccgaaggc
     6181 atcgtgatgg gcggcgagca gagcgggcat ctgatcttcc tggaccggca cacgaccggg
     6241 tgcggcatcc tgtcggccct gcggctgctg ggcatcatgg ccggcaccgc gatgccgctg
     6301 tcccggctgg cggcgtcggc gccgcgtttg ccgcaggtcc tgctgaacgt gcgcgtcgcg
     6361 cagcgcggag ggctcgacga cgcgacggac gtctgggacg aggtccgccg ggtcaccgcc
     6421 gagctcggcg aggacggccg tgtcctggtg cgcgcctccg gcaccgagcc ggtcgtgcgc
     6481 gtgatggtcg agtcctcgga ccaggatcgg gccgcggcgg ccgccgagcg gatcgccaag
     6541 gccgtcaccg acgcgctcgg ataagcaagg ttcggcccgg gcgccagcgg gtagactgac
     6601 ggcgtgtgcg ggatcatcgg gtacgtcggt gaacagcagg ccctccccat cctgatggat
     6661 gcgctgcggc gactggagta ccgcggatac gactcggccg gcgtggccgt cctcgacggc
     6721 ggcctccagg tggtccgcaa ggccggaaag ctcgacgagc tggacaagga gcttgcgccc
     6781 tcgggccttc acggaaccac cggcatcggt cacacgcgat gggcgaccca cggcccgccc
     6841 accgacgaaa acgcgcaccc ccacagcgac tgcgacggcc gcatcgccgt gatccacaac
     6901 ggcatcctcg aaaacttctt ggagcttcgc gccgaactgg aggccgaagg gcacacgctc
     6961 ggcagtgaca ccgacaccga ggcgatcgcg cacctgatcg aagccgtcta cgacggcgac
     7021 atcgtcgccg caacccgcaa ggccctcacc cggctgtcgg gcgcgttctc gatcgcggtc
     7081 gtggccgccg gcgagcccga cgtggtcgtg gccgccaagc gaacctcgcc gctgatcgtc
     7141 ggacgcggcg accacggaac gctgctggcg tcggacccca ccgcgctgat cccctatacc
     7201 cgtgacgtcg tgcaccttct cgacgaccag gtcgcagtga tccgccggga aggattcgag
     7261 atcaccacgc tcgacggcgc gcccgccgaa ggcaccgcga tccacatcga ctgggacctg
     7321 gaggccgccg agaagggagg ctacgacgac ttcatgctca aggagatcta cgagcagccc
     7381 ggagcggtcg ccgactcctt gcgcggccgg ctgcgcgggg gccgcctgca cctagacgag
     7441 gtccgcctga gcgaagagga gctgcgcggc atcgacaagg tcttcgtcgt cgcctgcggc
     7501 agcgcctaca acgccggcct cgtggccaag tacgcgatcg agcactggtg ccgcgtcccg
     7561 gtcgagatcg acatatccag cgagttccgc tatcgggatc ccgtgctcgg caaggacacg
     7621 ctcgcggtgg cggtatcgca gtccggcgag acgatcgaca cgctcgaggc ggtgcgccat
     7681 gcgcgccggc agcgcgcgaa ggtgatcgcg gtctcgaaca cggtcggatc gtcgatcccg
     7741 cgcgaggcgg acggtgtgct ctacacccag gccggcccgg aggtcggcgt cgccgcgacg
     7801 aagacgttca tcacgcagat ggtggtgctc aagctgctcg cgctctacct cgcgcaggtg
     7861 cgcggcacga tctatccgga ggagatcgca cgcatcgtcg aggacctcga gtcgctcgaa
     7921 ggcaaggtcg agacggtgct cggctcgcgg cagcaggtcc ggggcatcgc ggaggagatt
     7981 catcaggcgg ccgacgtgct gttcctgggc cgccatgtcg gtttcccgct cgcgctggag
     8041 ggtgcgctca agctcaagga gatctcctac atccacgccg agggctacgc ggccggcgag
     8101 ctcaagcacg gcccgatcgc gctgatcagc gaagggacgc ccgtggtggt cgtggcgacc
     8161 caatgccacg tctatgcgaa ggtgatcgcc aacatccaag aggtcaaggc gcgcggcgcg
     8221 cgtgtgatcg ccgtcgcgac cgagggcgat accgaggtcg ccaagcacgc cgaccacgtg
     8281 atcacggttc cttccacgca cgagctgctc acccccgagc tggtcgtggt gccgctgcag
     8341 ttcctgtcct acgagatcgc gaagatgcgc ggctgcgacg tggacaagcc gagaaacctg
     8401 gcgaagtccg tcaccgtgga gtagtccgtg ctcgatgcgc tgacggtcga agacgtccgc
     8461 cgccaggacg ccgagtgcga ggcgcgcggc atcccggtcg agcggctgat ggagcgagcc
     8521 ggcttcggcg tcgcccgcgc cgcacgcgcg atgctcggct acagctacgg gatgcgtgtc
     8581 gtggtggtgt gcggcaaggg aaacaacgcg ggcgatgggc tcgtggccgg ccgctggctc
     8641 gcgcactggg gcgcgcacgt cgtggccgtc atggcgctcg gccgcgagct tcgcggcgcg
     8701 gccggcgcca acctggcgcg ctttcccggc ccggtcgtgg acgccggcgc tttggaccgc
     8761 gagctggcgc gcgccgatct ggtgatcgat gcgctgttcg gcgtgggcct ttcgcgtgcg
     8821 cccggcggcg aggccgcgcg cgccatcgat gccatcaacg gcgcgggggc gccggtcatc
     8881 tcgatcgacg tgcccagcgg catggacgcc gacaccggcg acgcgcccgg cgccgtggtg
     8941 cgggccgacg gcatcgtcac gctcggcggg ctcaagcccg gcctgctgtt ccatgccgac
     9001 gtcgccgact gggtcgagat cgccgacatc ggcgttccgc gcgacctgcg gatcggcagc
     9061 gcgctcgtcc cggaggcgag cgacgtcgcg tcgctgctgc cgctccgggc ccccggcggc
     9121 cacaagcgcg actcgggcac cgtgctcgtt gtcgccggct cgcgggcgat gcccggcgcc
     9181 gccgcgctga cggccggcgc gtgcgtgcac gccggtgcgg gcctgacgac gctggccgcg
     9241 accgagcgcg tgaccgccgt tgcgttatcc cgcgttcccg agatgaccac gatcgtgctg
     9301 cccgactcgc ccgagggcgc gctcgacgac aaaggcgccg agatcgtgct tgagcgggcg
     9361 gaccagttct cggccatcgc gatcggcccc ggcctgtcgc ggcatccggc cacggccgaa
     9421 gccgtgcgaa ggatcctgct gtccaccgac gtgcccgcgg tcgtggacgc ggacgcgatc
     9481 gtcgcgttcg ccggcgggcc gggcgcgctg cgggagcgtc gcggcttcac ggtgctcacg
     9541 ccccacggcg gcgaggccgc cgcgctgctc ggcgccgagg cggcggatct gcgcgcggac
     9601 cggatcgccg cagcgcggcg catcgcgcgc gagaccggtc aggtcgcgct gctgaaaggg
     9661 ccggggacgg tggtgtgcgg cgaggacggc cccgtgtatc tgaacgcgac cggcaaccgc
     9721 gggctggccc aaggcggaac cggcgacgtg ctcaccggcg tgatcgcggc gctgctcgcg
     9781 cagggtggtg acgcacccga cgagacc
//
LOCUS       DATLFO010000109        14565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_131357, whole genome shotgun sequence.
ACCESSION   DATLFO010000109 DATLFO010000000
VERSION     DATLFO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131357"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VM841_03965"
     CDS             complement(<1..445)
                     /locus_tag="VM841_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HVL89373.1"
                     /translation="MKFIWDGGEVTGTWDVPESGTRAALILGHGAGSDYRAKLLQAVA
                     AGMASRGIATLRFNFPYTEAGKKMPDPQLKLEACFRAVAEKVASQITPVFLGGKSLGG
                     RIASHVAASGFDAAGLVFLGYPLHPPGKPDKIRDAHLPGIACPMLW"
     gene            complement(442..1302)
                     /locus_tag="VM841_03970"
     CDS             complement(442..1302)
                     /locus_tag="VM841_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL89374.1"
                     /translation="MDSKTLNLLVHDREAAVYDERFAIDYSRIARAIRLDMEWLLGPA
                     PAPARRALDFCCGTGYAAIGLAAAGYAAEVHACDLSGAMLDRTQKNAAEAGVRVTATQ
                     CDGEALPYADASFDLVVARGALHHLPSPLEALREVRRVLAPGGTAVVLAEPTPAGEKQ
                     VAAVIGTLARGLDAVRALRGVTKDAEHHHWELASMAANLHTFVPADLELMARAAGFED
                     VRVETAWWCWILALGLNYYLSGESKLIESNRLVRRIRYGLVDGAQMLDRTAGALVPSR
                     WRHTVQAVMR"
     gene            complement(1339..2406)
                     /gene="rlmN"
                     /locus_tag="VM841_03975"
     CDS             complement(1339..2406)
                     /gene="rlmN"
                     /locus_tag="VM841_03975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HVL89375.1"
                     /translation="MNPTDLFALTRAEWRAFAADIGEPAFRGEQIHRWMHVDLAGSFD
                     EMTNLSKPLRAGLTERFGAAFAPPAIVQTGDRGTTVKALFRFDGGALAEAVLMAYDDR
                     ATVCISSQAGCGMGCPFCATGQMGLLRNLSPGEIVSQVLWAARTLRGLDLPEDWTRRL
                     SNVVFMGMGEPLANFDRVWESVERIHDSEGFNLSARGITISTVGVLPGMRRLAAAGLP
                     VTLALSLHAPNDELRSTLVPLNERYPIKALLAAAREHRNAHGRRVSIEYAMIGGVNDS
                     DDHARELARLLRGDDFHVNLIPLNPTPGFEGPGSPLKQIERFQSLLESGGINATVRRN
                     RGTDIDAACGQLGARAVAAGA"
     gene            2460..3392
                     /locus_tag="VM841_03980"
     CDS             2460..3392
                     /locus_tag="VM841_03980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HVL89376.1"
                     /translation="MPEQPCAFHPDRPTLVTCSRCDRPICPEDMIPAAVGNQCPVCAG
                     RMREGPLGQTAYRLRNKAETVPVLRRVSGQRLTGLIVGINVVVFVLMLATGEPQSQRT
                     LVRFGALVRPLPEGEWWRIFSSMFVHIGLIHVGFNMFALAIFGPPIEERYGKGRFLAL
                     YFGSGLLGAAASLTFNAGFGIAAGASGAVFGILGAWIAFFVRHRDLSGARQQLNSLLM
                     LVGINLMLGLVGPIDNFAHLGGLVGGFVMGTVLEVSAKMRDGRVVAAVAALVFVAAVS
                     AALIVPRTCPAGSVLTLDDGRPVPCELFTGGFLR"
     gene            3429..4346
                     /locus_tag="VM841_03985"
     CDS             3429..4346
                     /locus_tag="VM841_03985"
                     /inference="COORDINATES: protein motif:HMM:NF042562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6687 family protein"
                     /protein_id="HVL89377.1"
                     /translation="MRYVPYEEMGGEPNIVVDGTGTDGTVLVLSHWRGSGTPETLADD
                     LSTQIAFHYLDRPEIHVAAEMVSNNHFDEDGLCGIYAIVHPEDAVRRREQLIAVARAG
                     DFGVARDRDVARVSMTLMAFEDEERSPLPPETLSLPYEQFSGVLYRETLALLPRLLDD
                     IEPWRHLWGDEDAHLSATLKAIHDGTISIEEHPEIDLAIVTVDGFDADPHEMAVNTST
                     DCFRVLTVGVDEPTFRLRYETWVDYRSRATMPRPEMKPLAVRLSDKDEIEWLAGSIGG
                     LTPDTRPDGETALEPKQIVEIVTAYLAGS"
     gene            4406..5245
                     /locus_tag="VM841_03990"
     CDS             4406..5245
                     /locus_tag="VM841_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL89378.1"
                     /translation="MLVSREEMTYDFEGASFDLERDRGVLGWIFSQFLYGEVTGVQVG
                     HWLERAPDFEAAQFLARQANEEMAHVKVFLRILSSLGQQPQPPNRVLKFLVTDFAGAT
                     FTEHCFVEMALGEGFVLGAIYALVDTLPESDIRTMLKGLARQEEGHVSFGEEQTIKAL
                     RRDPAQARHLLGLALVSMAGVGKLAGYARARFADHPVLSQLPGFLAHTRKVTELRLHR
                     MGVARNGTLPAMKPADRGMLIAASVARRYGRALNPLRQRPRSLTGTYLRDPSIRERLR
                     SGE"
     gene            5292..6824
                     /locus_tag="VM841_03995"
     CDS             5292..6824
                     /locus_tag="VM841_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89379.1"
                     /translation="MRSRRVAALAAIVAMLAVACGNRLPKTAAGGSLLPGETVPTGAA
                     GSPVVPGPGETVAPGGPAAGRDPARGFGARGCIPMASDPDRGVTDSEIKVGTIVARSG
                     PLPGSFYPMTQAIAAYFKKVNDAGGICGRTLRLIVQDDRLNADAHLTAAKKLVESDKV
                     FAIVGMLTAVDKASAAYLCARGVPDVGGFALSYNRAQGEDCPAGKPPVYWSPLGGLTR
                     ATIGDHAYYTIQAAEKLGGAGRRGAVMYHSTLDISKDQGLAQQWAVNHAWCRLYKHAS
                     ATDATASPCDVVASRQDKTYVPDDQLYDVNPVATDAAYDPLVQRMISANVDAVYSSME
                     INSNIKLLRSMGRYKSAWRQRLGRDPVVYFQLSAYDPKLLAEAAAQALGSYLWIPHVP
                     FTEPQHSVMGDYLSTLAKFFPTAEATSFGAQGWAGAAMFVEAVARAGANLTRESLRAA
                     LDSISGYTAGGFVGSLTPRERTIFHCGVVVRVESRSGRTDFYRWRPERDGFLCTTLKR
                     WR"
     gene            6821..8266
                     /locus_tag="VM841_04000"
     CDS             6821..8266
                     /locus_tag="VM841_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL89380.1"
                     /translation="MIEAVRGIFAGGDWMRASRGATITVTDPALEETIATVPRGAAAD
                     ARTCVHAARWAFDEGPWPRMTPAERAAHLKRFAVEATIDRPAFVELAVREWGVPVAGA
                     DEGFVRPSLEAFAWYAERASRDPESRLPDHGDTSSTVVREPAGVVAVLAAAAEPLAAI
                     VHKAIPALAAGNTVVVKAASAAPLSAFALARCAQRAGMPPGVFNVVSGSARDAGDELA
                     SNAMVDVVALAGSIATGRRVAARAAGTVKTCLFTLGASGTAVVLDGSDPAVVAPSLVA
                     AWLARAGQTWGAPCRLVVTAAVHDQLLDAIVAEVKGMPVGDPADPSIRVGPVRAAKAR
                     DRAEALIGAAVAAGASLVAGGSRPGDLSRGFFLEPAVVTGVRPGMGVFGEEVAAPVLA
                     VVAAGGDEDAIRIANERIDGVSAMVWSEGRDRALAAARRLRAGTVLAQGAGANPWAPA
                     GGYGQAGVGRERGLAGMDAYSEIKHVAGPAT"
     gene            complement(8274..8564)
                     /locus_tag="VM841_04005"
     CDS             complement(8274..8564)
                     /locus_tag="VM841_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89381.1"
                     /translation="MQNEHADLTANDPEGLGRAAREGMRALVLNVLERKSVSKDDLEA
                     CPVCGDVIIHSATPESYVKDRALGNVICAPCGTERSVLHMVLPDNVDIGGPG"
     gene            complement(8590..9441)
                     /locus_tag="VM841_04010"
     CDS             complement(8590..9441)
                     /locus_tag="VM841_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVL89382.1"
                     /translation="MQSGPMNATTIFSARLDAPPSQGVVAARDGRSAAWMAAAWSVVT
                     GRTGHVAVSDADDKALAHLVAARARVTLSIGSPERTAGEADDAHACLIADARSPAAPV
                     DEWRAIDARPIRLERLAADLTRVLPPGAVVCVEVADLEPVVLAAAAAHPSVLALSRLS
                     GLPRGAAIAWAIGAKAAAPSRTVVALTDAPGFAIGGMDLATAARHDLPVAVVVANAEH
                     DLPAFELVAPIAGGHGEQVTDPSRLAIHLRNAMSVPISSVINVAVEAPRTAPRRHPVA
                     RGSRLMA"
     gene            9556..10200
                     /locus_tag="VM841_04015"
     CDS             9556..10200
                     /locus_tag="VM841_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89383.1"
                     /translation="MSRKLATIVAALAVAAPAGGTFASHAQPPQYVDAPGVTITITSE
                     SSGDEVYHDRNTAGRSGTKCTAAGSDDPNDNARECWNDRSQLRDKDVTNDQGTPASCR
                     RIQGQTVPGVATWDGVWFNRTPSGTIPDTCGDPTTGGAKGRVYTGAAATSVGSAGVAG
                     GGDLVSGGCVQGFVEDDTPGNLVSSAVTQAQVLAGQPHSGGDDDTDAGVCFDYL"
     gene            10307..12247
                     /locus_tag="VM841_04020"
     CDS             10307..12247
                     /locus_tag="VM841_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89384.1"
                     /translation="MDLIAAIWPLLVIGLVVGSVYSIAAAGLVLTYRASGVFNFGHGA
                     IGAFSAFFYAWCVQRVGLPIGVAAVITMAVMAPLLGIALERSIFRRLREAPTVVAIVA
                     SIGVLVALQGAAAALWGRSGLSAQRIFPGRVFQIGDRVSIGADQAGVVGVGIVVSLLL
                     WVVLKRTKMGIAMRAVVDRPDLVETMGYSADRISSASWAMGASLAAAAGILLTPMVSL
                     EVYTLTLIVINAYAAAMVGRLESLPLTFAGGVLLGFGEALVGRFLPQGIAAGVLPSLP
                     FALLLGLLIIPRTRPLREPRAAGIAASLGAWIPPLRREWPAKKRLGIVALIAAGMITL
                     ARLGADVTFAVDLALVHAILFLSLVLLVGYGGQISLAQAALAGVGGIVTAHLVTNLGW
                     SWFAALPVAGLAAVPVGALLAYPALRLHGLFLGLATMAFSLLMDNAIFTRDALTGGSS
                     GIAVPRPGVLQSDVRFGVVLTLIFALLAWGLHNLRRGRTGRILAAMRDSETGARSIGI
                     SPARTKLILFMMGAFIAGIGGSTYVAAFQRVDAGQFIVFYSIVWLAVAVIGGISTWAG
                     ALAGGLVYSIVPFLFDRIGHPAASDMIPVLFGAAAILLARNPRGIVTVPRRVREMVAG
                     WMSRALAPFEEPADEPQEVRSA"
     gene            12240..13046
                     /locus_tag="VM841_04025"
     CDS             12240..13046
                     /locus_tag="VM841_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89385.1"
                     /translation="MPRRARTRPAGPDALVVADATVRFGGVVALDGVSLHVRSEGVTG
                     LIGPNGAGKTTLFGVAGGFVTPDAGRVFFHDRDVTGWPAHRRAAAGIGRTFQRLELFG
                     RLSALENLLVAREVRRSKATLFDDLFALRRAATHEHAARDAAEQILSLVGLEWARDRE
                     AASLPLGAGRLLEIGRALCAEPSLLMLDEPTSGLDARESEMVASLIGRVRTQLSVSVL
                     LVEHDVDFVASVCDDVFVLDFGRMIAHGPPDRIGKHPVVRAAYLGAEAAS"
     gene            13043..13744
                     /locus_tag="VM841_04030"
     CDS             13043..13744
                     /locus_tag="VM841_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89386.1"
                     /translation="MSVLLSVEDLTVAYGKIRAVDGVSLVVPRGSVVALLGPNGAGKS
                     SLLRAIAGLAPREGRIVLGGEPIHDLPAHAIARRGLCSIPEGRGVFGALTVAENLRMA
                     STDDRDWTARVVEAFPILGERMGQQAATLSGGEQQMLALARAVASEAPLVTIDEPSLG
                     LAPKMVGAIYAAIRRMRERHRTVLLVEQYAAQALPVADLVAVMAHGRIEFFGEPGELA
                     YAPPLVEQYLGRITA"
     gene            13798..>14565
                     /locus_tag="VM841_04035"
     CDS             13798..>14565
                     /locus_tag="VM841_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89387.1"
                     /translation="MRPVRRARSGRLLAVGAASCLLAATFTPGAIASDIPASYDAVAE
                     SAAFQVRVLKPAALPTSDELATIAIPFSHAEASSSPLGRAIGSWLWPGETVADVKSLA
                     LVGLDPGADETACAERQDGSDKPRTLALVPNPAGGEIAILDPADNPCGSASFKQIFAG
                     QIDNDEGSPTRGQRTGGVIRAMPDYPFWSVAQYPSPSGRSDADRKVLCQAPGAIPDEP
                     WHTPRDACPPGGMPDAGVSSAYASGMPPGGQASRGVCH"
BASE COUNT         1999 a         5087 c         5206 g         2273 t
ORIGIN      
        1 gccacagcat cgggcaggcg atgccgggca ggtgcgcgtc gcggatcttg tcgggcttgc
       61 ccggcggatg cagcggatag ccgaggaaga cgagcccggc cgcgtcgaag ccggacgcgg
      121 ccacgtgcga ggcgatgcgg ccgccgagcg actttccgcc gaggaacacc ggcgtgatct
      181 gcgaggcgac cttctccgcg accgcgcgaa agcaggcctc gagcttgagc tgtggatcgg
      241 gcatcttctt gcccgcttcc gtgtagggga agttgaagcg cagcgtcgcg atgccgcgcg
      301 aggccatgcc cgccgccacg gcctgcagca gcttggcgcg atagtcgctg ccggcgccgt
      361 gaccgaggat caacgcggcg cgcgtgcccg actcgggcac gtcccaggtg ccggtgacct
      421 cgccgccgtc ccagatgaac ttcatcgcat caccgcctgc acggtatggc gccatcggga
      481 cggcaccagc gcgccggccg tgcggtcgag catctgcgcg ccgtccacga gaccgtagcg
      541 gatccggcgc acgagccggt tggattcgat cagcttcgac tcgcccgaca ggtagtagtt
      601 gagcccgagc gccaggatcc agcaccacca tgccgtctcg acacgcacgt cttcgaagcc
      661 cgcggcacgc gccatcagct cgaggtcggc gggcacgaac gtgtgcaggt tcgccgccat
      721 cgatgcgagc tcccaatggt ggtgctcggc gtctttcgta acgccgcgca gtgcgcgaac
      781 ggcgtcgagg ccgcgcgcga gcgtgccgat caccgcagcg acttgcttct cgcccgcggg
      841 cgtcggctcg gcgagcacga ccgcggtgcc gccgggtgcg agcacacgcc gaacctcgcg
      901 cagggcctcg agcggcgacg gcaggtgatg aagcgcgccg cgcgccacca ccagatcgaa
      961 cgacgcgtcc gcgtagggga gcgcttcgcc gtcgcattgg gtagcggtga cacgcacgcc
     1021 ggcctcggcc gcgttcttct gcgttcgatc cagcatcgcg cccgacaggt cgcaggcgtg
     1081 cacctcggcc gcatagccgg cggcggcgag cccgatcgcg gcgtatccgg tgccgcagca
     1141 gaagtccagc gcgcggcgcg ccggtgccgg cgccgggccg agcagccatt ccatgtcgag
     1201 ccggatggcc cgcgcgatcc gagagtagtc gatcgcgaag cgctcgtcgt agacggccgc
     1261 ttcgcgatcg tggacgagca ggttcaaggt cttggagtcc atggtgcgcc gagtctacgc
     1321 gacggcgtgg ctcgcggact acgcgcccgc cgcgaccgcg cgcgcgccga gctggccgca
     1381 tgccgcgtcg atgtcggtgc cgcggttgcg gcgcaccgtc gcgttgatgc caccggattc
     1441 cagcagcgac tggaagcgct cgatctgctt cagcggcgag ccgggccctt cgaagccggg
     1501 tgtcgggttg agcgggatca gattgacgtg aaagtcgtct ccgcgcagca gccgcgccag
     1561 ctcgcgcgcg tgatcgtcgg agtcgttgac gccgccgatc atcgcgtact cgatcgagac
     1621 gcgccggccg tgcgcgttgc gatgctcgcg cgcggcggcc agcagagcct tgatcggata
     1681 ccgctcgttg agcggcacca gcgtcgagcg aagctcgtcg ttgggtgcgt gcagcgacag
     1741 cgccagcgtc accggcaggc cggctgcggc gagccgccgc atgccgggca gcacgccgac
     1801 cgtcgagatc gtgatgccgc gcgccgacag gttgaagccc tcggaatcgt ggatccgctc
     1861 gaccgattcc cagacgcggt cgaagttggc cagcggctca cccatgccca tgaacaccac
     1921 gttggacagc ctccgcgtcc agtcctcggg aaggtccagg ccgcgaagcg tgcgcgcagc
     1981 ccacagcacc tgcgacacga tctcgccggg cgataggttg cgcagcaacc ccatctggcc
     2041 ggtcgcgcag aacggacagc ccatgccgca gcccgcctgc gacgagatgc agacggtggc
     2101 gcggtcgtcg taggccatca gcaccgcttc ggccagcgcg ccgccgtcga agcggaacag
     2161 cgccttgacg gtcgtgccgc ggtcgccggt ctgcacgatc gccggcggcg cgaacgccgc
     2221 gccgaaccgt tcggtcaggc ctgctcgcaa cggcttcgac aggttggtca tctcgtcgaa
     2281 cgagcccgcc aggtccacgt gcatccaccg gtggatctgc tcgccgcgga acgcgggctc
     2341 gccgatgtcg gccgcgaacg cgcgccattc ggccctggtc aaagcgaaca gatcggtggg
     2401 attcataggg tcagtctaga cgtccggcgg agcgtatgac gggccgatag actcgcgtca
     2461 tgcccgagca accctgcgcc tttcatcccg atcgcccaac gctggtcacg tgctcccgct
     2521 gcgatcgtcc catctgcccg gaggacatga tcccggcagc cgtcggcaat cagtgtccgg
     2581 tgtgcgccgg ccggatgcgc gaagggccgc tcggccagac cgcgtaccgt ctgcgaaaca
     2641 aggccgagac ggttccggtt ctgcgtcgcg tgagcgggca gcggctgacc ggcctcatcg
     2701 tcgggatcaa cgttgtggtg ttcgtgctga tgctggccac cggagagcca cagtcgcagc
     2761 gcaccctggt tcgcttcggg gcgctggtcc gtcccctgcc ggaaggcgag tggtggcgga
     2821 tcttctcgtc gatgttcgtg cacatcggcc tgatccacgt gggtttcaac atgttcgcgc
     2881 tggcgatttt cggtccgccg atcgaggagc gctacggcaa gggccgcttc ctcgccctct
     2941 acttcggcag cggccttctg ggcgccgctg cgtcgctgac gttcaacgcg ggtttcggca
     3001 tcgccgcggg cgcgtcgggc gcggtcttcg gcatcctcgg cgcgtggatc gcgttcttcg
     3061 tgcgccaccg cgacctgtcg ggcgcgcgtc agcagctcaa cagcttgctg atgctggtcg
     3121 gcatcaatct catgctcggc ctggtcggcc cgatcgacaa cttcgcgcat ctggggggac
     3181 tggtgggcgg cttcgtgatg ggcacggtgc tcgaggtttc ggcaaagatg cgcgacggcc
     3241 gcgtggtggc cgccgtcgcc gcgctcgtgt tcgtggccgc cgtctccgcg gccttgatcg
     3301 tgccgcggac ctgcccggcc ggcagcgtgc tcacgctcga cgacggccgg cccgttccct
     3361 gcgagctgtt caccggcggg ttcctccgat agcaggtccg gtcgcgaagc cgggtgtacg
     3421 gtagccccat gcgatacgtg ccgtacgagg agatgggcgg cgagccgaac atcgtcgtgg
     3481 acggaaccgg aaccgacggc acggttttgg ttctgagcca ctggcggggg agcggaacgc
     3541 ccgagacgct cgccgacgac ctgtcgacgc agatcgcgtt ccactacctg gaccgccccg
     3601 agatccacgt cgccgccgag atggtcagca acaaccactt cgacgaggac gggctctgcg
     3661 ggatctacgc gatcgtgcac ccggaggatg cggttcgccg ccgcgagcag ctcatcgccg
     3721 tcgcgcgcgc gggtgacttc ggggttgcgc gggaccgcga cgtggcgcgt gtatcgatga
     3781 cgctgatggc cttcgaggac gaagagcgct cgccgctgcc gcccgagacg ctcagcctgc
     3841 cgtacgagca gttctcgggc gtgctgtatc gcgagacgct cgcgctgctt ccgcgtttgc
     3901 tcgacgacat cgagccttgg cggcacctgt ggggtgacga ggacgcgcac ctgagcgcga
     3961 cgctgaaggc gatccacgac ggcacgatct cgatcgagga gcatcccgag atcgaccttg
     4021 cgatcgtgac ggtggacggc ttcgacgccg atccgcacga gatggcagtc aacacctcga
     4081 ccgattgctt ccgggttctg accgtcgggg tggacgagcc gaccttccgt ctgcgctacg
     4141 agacctgggt cgactaccgc tcgcgggcca cgatgccgcg ccccgagatg aagccgctcg
     4201 ccgtgcggct gtccgacaag gacgagatcg agtggctcgc cggcagcatc ggcggcctga
     4261 cccccgacac ccggcccgac ggcgagaccg cgctcgagcc gaagcagatc gtcgagatcg
     4321 tgaccgccta cctggccgga tcctgagccg agagtgcgcg agccgcgccg ggcaggatcg
     4381 gcgtgcagcg gctagcatcg attccatgct ggtctcgcgc gaagagatga cctacgactt
     4441 cgagggcgcg tcgttcgacc tggaacgcga ccgcggggtg ctcggatgga tcttctcaca
     4501 gtttctctac ggcgaggtga ccggcgtgca ggtggggcac tggctcgagc gcgcacccga
     4561 cttcgaggcc gcgcagttcc tggcgcgcca ggccaacgag gagatggcgc atgtcaaagt
     4621 gttccttcgc atcctgtcct cgctcgggca acagccgcag ccgccgaacc gggtgctgaa
     4681 gttcttggtg accgacttcg ccggcgccac cttcaccgag cattgcttcg tcgagatggc
     4741 gctcggcgaa ggcttcgtgc tcggcgcgat ctacgcgctg gtggacaccc tgcccgagtc
     4801 cgacattcgt acgatgctca agggacttgc gcggcaggag gaagggcacg tgtccttcgg
     4861 cgaggagcag acgatcaaag cgctgcgccg cgaccccgcc caggcacggc atcttctggg
     4921 gctcgcgctg gtgtccatgg ccggggtggg caagctcgcc ggctacgcac gcgcacgctt
     4981 cgccgaccat ccggtcctgt cgcagcttcc cggcttcctt gcgcacaccc gaaaggtgac
     5041 cgagcttcgg ctgcatcgca tgggcgtggc ccgcaacgga acgcttccgg cgatgaagcc
     5101 cgcggaccgg ggcatgctga tcgccgcatc cgtcgcgcgg cggtacggcc gcgcactcaa
     5161 cccgttgcgg caacggcctc gctcgctgac cggaacctat ctgcgcgacc catccattcg
     5221 cgagcgcctg cgcagcggag agtaaaagcc gcccttcttg tcccggcccg tccgggttac
     5281 catggattcg tatgaggtct cgccgtgttg ccgcgctggc cgccatcgtc gcgatgctcg
     5341 ccgtcgcctg cggcaaccgg cttcccaaga cggccgcggg tggctcgctg ctgccgggag
     5401 agaccgtccc cacgggcgcc gccggcagcc ccgtcgtccc gggtccgggc gaaaccgtcg
     5461 cgccgggcgg gccggcggcc ggccgcgatc ccgcacgcgg cttcggcgct cgcgggtgca
     5521 tcccgatggc ctcggatccg gaccgcggcg tcaccgactc cgagatcaaa gtcggcacga
     5581 tcgtcgcgcg gtcgggtccg ttgccgggct cgttctatcc catgacccaa gcgatcgcgg
     5641 cctacttcaa gaaggtcaac gacgcgggcg gcatctgcgg ccggacgctg cgcctgatcg
     5701 tccaggacga ccggctcaac gccgacgccc acctgacggc cgcgaagaag cttgtcgaat
     5761 ccgacaaggt tttcgcgatc gtggggatgc ttaccgcggt agacaaggcg agcgcggcgt
     5821 acctgtgcgc ccgcggcgtt cccgacgtcg gcggcttcgc gctgtcctac aaccgggcgc
     5881 agggcgagga ctgcccggcc ggcaagccgc cggtgtactg gtcgccgctc ggcgggctca
     5941 cgcgcgcgac catcggcgat cacgcgtact acacgatcca ggcggccgag aagctcggcg
     6001 gcgccggtcg ccgcggcgcg gtgatgtacc actcgacgct cgacatctcc aaggaccagg
     6061 ggctcgccca gcagtgggcg gtgaaccatg cgtggtgccg tctgtacaag cacgcgtccg
     6121 caacggacgc gaccgcctcg ccgtgcgacg tggtggcctc gcggcaggac aaaacctacg
     6181 tgcccgacga ccagctctac gacgtcaacc cggtcgccac cgacgcggcc tacgatccgc
     6241 tggttcagcg gatgatctcc gccaacgtcg acgcggtgta ctcgagcatg gagatcaact
     6301 ccaacatcaa gctgctccgc tcgatgggcc gctacaagag cgcgtggcgt cagcggctcg
     6361 gccgggaccc ggtcgtctac ttccagctgt cggcctacga tcccaagctg ctcgcggaag
     6421 ccgccgcgca ggcgctcggc tcgtacctgt ggattccgca cgtgccgttc accgagccgc
     6481 agcactccgt gatgggcgac tatctgtcca cgctggccaa gttcttcccg accgcggagg
     6541 cgacgagctt cggcgcgcaa ggctgggcgg gcgcggcgat gttcgtggag gccgttgccc
     6601 gcgccggcgc gaacctgacg cgcgagtcgc tgcgcgcggc gctcgactcg atcagcggct
     6661 acacggccgg cgggttcgtc ggctcgctta ccccgcgcga gcggacgatc tttcactgcg
     6721 gcgtggtcgt gcgcgtcgag agccgcagcg gccgcaccga cttttaccgg tggcgccccg
     6781 agcgcgacgg ctttctgtgc accacgctga agcggtggcg atgatcgaag cggtacgcgg
     6841 catcttcgcg ggcggcgact ggatgcgcgc ctcgcgaggc gcgaccatca cggtgaccga
     6901 tccagcgctc gaggagacga tcgcgaccgt gccgcgcggg gcggccgccg atgcgcgcac
     6961 ctgcgtgcac gctgcgcggt gggccttcga cgagggaccg tggccgcgca tgacgcccgc
     7021 ggagcgagcc gcacacctga agcggttcgc ggtcgaggcc accatcgacc ggccggcgtt
     7081 cgtcgagctc gcggtccgcg agtggggcgt tcccgtcgcg ggggccgatg aggggttcgt
     7141 gaggccctcg ctcgaggcgt tcgcgtggta tgccgagcgc gcctcgcgtg atcccgagtc
     7201 gcggctgccc gaccacggtg acacctcctc gaccgtggtg cgcgagccgg ccggggtcgt
     7261 cgccgtgctc gcggccgcgg ccgagccgct cgcggcgatc gtgcacaagg cgatccccgc
     7321 gctcgccgcg ggcaacaccg tcgtggtcaa ggccgcatcc gccgctccgt tgtccgcctt
     7381 cgcgctggcg cggtgcgcgc agcgggccgg gatgccgccc ggcgtcttca acgtcgtctc
     7441 gggctcggcg cgcgacgccg gcgacgagct cgcctcgaac gcgatggtgg acgtggtcgc
     7501 gctcgccgga tcgatcgcaa caggccgccg cgttgccgcg cgggccgccg gcaccgtcaa
     7561 gacgtgcctt ttcacgctgg gcgcgagcgg aaccgcggtc gtgctcgacg ggtccgatcc
     7621 cgccgtggtg gctccgtccc tcgtcgccgc gtggctcgcc cgtgccggcc agacctgggg
     7681 ggcgccttgc cgactggtgg tcactgccgc ggttcacgat cagcttttgg acgcgatcgt
     7741 cgccgaggtg aagggaatgc ccgtcggcga cccggcggat ccgtcgatcc gcgtcgggcc
     7801 ggtgcgggcg gcgaaggcgc gggaccgcgc cgaagccttg atcggcgcgg cggtcgccgc
     7861 gggagcctcg ctcgtggccg gcggtagccg tccgggcgat ctgagccgcg ggttcttcct
     7921 cgagccggcc gtcgtcacgg gagtgcggcc cggcatgggc gtgttcggcg aagaggtggc
     7981 ggcgccggtt ctggccgtgg tcgcggccgg cggtgacgag gatgccatcc gcatcgcgaa
     8041 cgagcggatc gacggcgtgt cggcgatggt ctggagcgag gggcgggacc gggcgctggc
     8101 ggcggcgcgg cggctgcgcg ccgggaccgt tctcgcgcag ggcgcgggcg cgaacccgtg
     8161 ggcgcccgca ggcggctacg ggcaagccgg cgtgggacgc gagcgagggc tcgcaggaat
     8221 ggatgcctac agcgagatca agcacgtcgc cggaccggcg acgtgagcgc ttcttagccc
     8281 gggccgccga tgtcgacgtt gtcgggcaga accatgtgca gcaccgatcg ctcggtgccg
     8341 cagggcgcgc atatcacgtt gccgagcgca cgatccttga cgtaggactc gggcgtcgcc
     8401 gagtggatga tcacgtcacc acacacgggg cacgcctcga gatcgtcctt cgagacgctc
     8461 ttacgctcga ggacgttgag cacgagggcg cgcattcctt cgcgtgccgc gcgccccagc
     8521 ccttcggggt cgttggcggt cagatcggcg tgctcgttct gcatcgatga cgctcctttc
     8581 gaagccccat catgccatga gccgcgagcc ccgcgcgacc gggtgcctcc gcggggccgt
     8641 ccgcggagcc tcgacggcga cgttgatcac cgacgagata ggaaccgaca tcgcgttgcg
     8701 aagatggatc gccagccgtg acgggtcggt cacctgctcg ccgtgccccc ccgcgatggg
     8761 ggccacgagc tcgaacgcgg gcaggtcgtg ctcggcgttg gccacgacca ccgccaccgg
     8821 cagatcgtgt cgcgcggccg ttgcgagatc catgccgccg atcgcgaaac ccggcgcatc
     8881 ggtcaaggcc acgacggtcc gcgagggcgc ggccgccttc gcgccgatcg cccacgcgat
     8941 cgccgcgccg cgcggaaggc ccgacagcct cgacagcgcg agcaccgacg gatgcgccgc
     9001 tgcggccgcg agcacgaccg gctcgagatc ggcgacctcg acgcacacca ccgcacccgg
     9061 cggcagcacg cgcgtgagat ccgcggcgag ccgttccagc cggatcggcc gcgcgtcgat
     9121 ggcgcgccac tcgtcgacgg gagccgcagg gctccgggca tccgcgatga ggcatgcgtg
     9181 cgcatcgtcg gcttcgccgg cggttcgctc cggggatccg atcgacagcg tcacacgcgc
     9241 gcgcgcggcg accaagtgag cgagggcttt gtcgtcggcg tcgctcacgg cgacgtgtcc
     9301 ggttcggccg gtcacgacgg accaggccgc ggccatccat gccgccgagc ggccgtcgcg
     9361 tgccgccacc acgccctgcg acggcggagc gtcgagcctt gccgagaaga tcgtggtggc
     9421 gttcatgggt ccgctctgca tgctacgtca gcggaccccg aatccagggc atgggggagg
     9481 gcgcccgccg ggcgaaggat ttcgccgctg cgccgtcgaa gtgtcgcacg gaccgctcgt
     9541 cgagggaggg atcggatgag ccgcaaactc gcaacgatcg tggcggcgct ggccgtcgcc
     9601 gctccggccg gtgggacgtt cgcgtcgcac gcccagccgc cgcagtacgt ggacgcgccc
     9661 ggtgtcacga tcacgatcac gagtgaatca tccggcgacg aggtctacca cgatcgaaac
     9721 acagccggac gcagcggcac caagtgcacc gccgccggca gcgacgatcc caatgacaac
     9781 gcccgtgagt gctggaacga ccggtcgcag cttcgcgaca aggacgtcac caacgatcaa
     9841 ggcacgcccg cgtcgtgccg gcgcatccag ggccagaccg tgccgggcgt cgcaacctgg
     9901 gacggggtgt ggttcaaccg cacgccttca ggcacgatcc ccgatacgtg cggcgacccg
     9961 accaccggcg gcgcgaaggg ccgcgtctac accggtgccg ccgcaaccag cgtcggcagc
    10021 gccggcgttg cgggcggcgg cgatctcgtg agcggcggct gcgtgcaagg cttcgtggag
    10081 gacgacacgc cggggaacct ggtgtcgtct gcggtgacgc aggcgcaggt gctcgccggg
    10141 cagcctcaca gcggggggga tgacgacacc gacgccggcg tctgcttcga ctacctgtag
    10201 cgcgcacccg gttttggggg cgcgttcccg ccgggacgcg ccctcgcatt tcggcggcca
    10261 tggcgttcgg cagtttctgc cgtagcatga gcggcggggg ccggatatgg atctgattgc
    10321 cgcgatatgg ccgttgctgg tcatcggact cgtcgtcggg tccgtgtact ccatcgcggc
    10381 cgccggtctg gtcctgacct atcgcgccag cggcgtgttc aacttcggcc acggcgccat
    10441 cggcgcgttc agcgccttct tctacgcctg gtgcgtgcag cgcgtggggc tgccgatcgg
    10501 cgtcgcagcc gtgatcacga tggccgtgat ggcgccgctg ctggggatcg cgctggaacg
    10561 gtcgatcttt cgacggcttc gcgaagcgcc cacggtcgtg gcgatcgtcg cctcgatcgg
    10621 cgtgctggtc gccttgcagg gcgcggccgc cgcgctgtgg ggccgcagcg gcctaagtgc
    10681 ccagcggatc tttcccggca gggtcttcca gatcggtgat cgggtctcga tcggagccga
    10741 ccaggcgggc gtcgtgggcg tcggcatcgt cgtgtcactg ctgctgtggg tggtgctcaa
    10801 acgcaccaag atggggatcg cgatgcgggc ggtcgtggac cggcccgacc tggtcgagac
    10861 gatgggatac agcgccgacc gcatctcgtc ggcctcgtgg gcgatgggcg cgtcgctggc
    10921 ggccgccgcg ggaatcctgc tcactccgat ggtctcgctc gaagtctaca cgctcacgct
    10981 gatcgtcatt aacgcctacg ccgccgcgat ggtcggccgg ctcgagtcgc tgccgctcac
    11041 gttcgcgggc ggcgtgctgc tgggcttcgg cgaggcgctg gtcggacgct tcctgccgca
    11101 aggcatcgcg gcgggcgtgc tgccgtcgct gccgttcgcg cttctgcttg gcctgctgat
    11161 cattccgcgc acgcgtccgc tgcgcgagcc gcgcgccgcc ggcatcgccg cgagcctggg
    11221 cgcgtggatc ccgccgctgc gacgcgaatg gccggcgaag aagcggctcg gcatcgtcgc
    11281 gctgatcgcc gccggcatga tcacgctcgc gcggctcggc gccgatgtga ccttcgccgt
    11341 ggatctcgcg ctggtgcacg ccatcctgtt tctgtcgctg gtgctgctgg tcggctacgg
    11401 cgggcagatc tcgctggcgc aagccgcgct cgccggcgtc ggcggcattg tgaccgcgca
    11461 tctggtcacg aaccttggat ggagctggtt cgcggcgctg ccggtggcgg gccttgccgc
    11521 ggtgccggtc ggtgcgctgc tggcgtatcc ggcgcttcga ttgcacgggc tgttcctcgg
    11581 gcttgccacg atggcgttct cgctgctgat ggacaacgcg atcttcacgc gcgacgcgct
    11641 caccggcggc tccagcggca tcgccgtgcc ccgtccgggc gtgctgcaat ccgacgttcg
    11701 cttcggcgtg gtattgacct tgatcttcgc gctgctcgcc tggggcttgc acaacctccg
    11761 acgcggccgt accggacgca tcctcgccgc gatgcgcgac agcgagaccg gcgcgcgctc
    11821 gatcgggatc tcgccggcgc gcaccaagct gatcctgttc atgatgggcg cgttcatcgc
    11881 cgggatcggc ggcagcacct acgtcgcggc gttccagcgc gtggacgccg ggcagttcat
    11941 cgtcttctac tcgatcgtgt ggctggcggt cgcggtgatc ggcggcatct cgacctgggc
    12001 gggcgcgctc gccggcggcc tggtctactc gatcgtgccg ttcctgttcg accgcatcgg
    12061 ccatccggcc gcgagtgaca tgatcccggt gctgttcggc gcggccgcga tcctgctcgc
    12121 gcgcaatccg cgcggcatcg tgaccgtgcc gcgccgcgtg cgcgagatgg tcgccggctg
    12181 gatgagccgc gcgctcgcgc ctttcgaaga gcccgccgac gagccgcagg aggtgcgcag
    12241 tgcctagacg agcccgcacg cgtcccgccg gtcctgacgc attggtcgtg gcggatgcga
    12301 cggtgcgctt cggtggtgtc gtcgcgctcg atggcgtgtc gctgcacgtg cgcagcgaag
    12361 gggtgaccgg cctcatcggg ccgaacggcg cgggaaagac gacgctgttc ggcgtcgcag
    12421 gcggcttcgt cacgcccgac gccggccgcg tgttcttcca cgaccgcgac gtcaccggct
    12481 ggccggcgca tcgccgcgcc gccgccggga tcggccgcac ctttcagcgg ctcgagctgt
    12541 tcggccgcct gtccgcgctc gaaaacctgc tcgtggcgcg cgaggtgcga cgctcgaagg
    12601 cgacgttgtt cgacgacctg ttcgccctgc gccgcgcggc gacgcacgaa cacgccgcgc
    12661 gcgacgccgc cgaacagatc ctgtcgctcg tcgggctcga gtgggcgcgc gaccgcgagg
    12721 ccgcgtcgct gccgctcggc gcgggccggc tgctcgagat cggccgcgcc ttgtgcgccg
    12781 agccgtcgct gttgatgctc gacgagccca ctagcggcct ggatgcgcgg gagtccgaga
    12841 tggtcgcgtc gctgatcggc cgcgtgcgga cccagctgtc ggtgagcgtg ctgctggtcg
    12901 agcacgacgt ggacttcgtg gcgagtgtct gcgacgacgt gttcgtgctg gacttcggac
    12961 ggatgatcgc gcacggtccg cccgatcgga tcggcaaaca ccccgtcgtt cgcgccgcgt
    13021 atctgggagc ggaggcggct tcgtgagcgt gctgctttcg gtcgaggacc tcacggtcgc
    13081 ctacggaaag atccgcgccg tggacggcgt gtcgctggtc gtgccccgag gcagcgtcgt
    13141 cgcgctgctc ggcccgaacg gcgccggcaa gtcgagcctg ctgcgcgcga tcgccggact
    13201 cgccccccgc gagggccgca tcgtcttggg cggcgagccg atccacgacc tgcccgcgca
    13261 cgcgatcgcg cgtcgcggcc tgtgctcgat tccggaagga cgcggcgtgt tcggcgcgct
    13321 gaccgtcgcc gagaacctac gcatggcatc gaccgatgac cgcgactgga ccgcgcgggt
    13381 cgtcgaggcg tttccgattt tgggcgagcg catgggccag caggctgcca cactgtcggg
    13441 cggcgagcag cagatgctcg cgctggcccg cgcggtcgca tccgaggcgc cgctggtcac
    13501 catcgacgag ccctcgctgg gcctggcgcc caagatggtc ggcgcgatct atgccgccat
    13561 ccgccggatg cgcgagcggc atcgcacggt gctattggtc gagcagtatg cggcgcaggc
    13621 gctgccggtc gccgatctgg tcgcggtgat ggcgcacggg cggatcgagt tcttcggcga
    13681 gccgggcgag ctggcctacg cgcccccgct ggtcgagcag tacctgggca ggatcacggc
    13741 gtgacgtccg aatctcgccg ctaccgcccg cgcgccccgt ttgcgacaat ggaaacgatg
    13801 aggcccgttc ggcgtgcgcg atccggccgc ctgctcgcgg tcggggcggc ctcgtgcctg
    13861 ctcgccgcca cgttcacgcc cggcgcgatc gcatccgaca ttcccgcgtc ctacgacgcc
    13921 gtcgccgagt ccgccgcgtt ccaggttcgc gtgctcaagc ccgccgcgct gccgacctcc
    13981 gacgagctcg ccacgatcgc gatcccgttc tcgcatgcgg aggcatcctc gtcgccgctt
    14041 ggccgcgcga tcggatcgtg gctgtggccg ggtgagaccg tcgccgacgt gaagtcgctt
    14101 gcgctggtcg ggctggatcc aggcgccgac gagacggcgt gcgccgagcg gcaggacggc
    14161 tccgacaaac cccggacgct cgcgctcgtg cccaatcccg cgggcggcga aatcgccatt
    14221 ctcgatcccg ccgacaatcc gtgcggcagc gccagcttca agcaaatctt cgccggccag
    14281 atcgacaacg acgaaggctc gcccacgcgg gggcagcgca ccggcggcgt gatccgcgcg
    14341 atgcccgact atccgttctg gtccgtcgcc caatacccgt cgccgtcggg ccgttcggac
    14401 gccgaccgca aggttttgtg ccaggcaccc ggcgcgatcc ccgacgagcc gtggcacacg
    14461 ccgcgcgacg cctgcccgcc cggcggcatg cccgacgcgg gtgtgtcgag cgcgtacgcg
    14521 tcgggcatgc cgccgggcgg gcaggcgtcg cgcggcgtgt gccac
//
LOCUS       DATLFO010000110         6605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_82566, whole genome shotgun sequence.
ACCESSION   DATLFO010000110 DATLFO010000000
VERSION     DATLFO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82566"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1057)
                     /gene="glpX"
                     /locus_tag="VM841_04040"
     CDS             complement(59..1057)
                     /gene="glpX"
                     /locus_tag="VM841_04040"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HVL89388.1"
                     /translation="MTPAKPDRNLALELVRVTEAAALAAAHWMGRGDKNAADAAAVDA
                     MRLMLNSVAIDGVVVIGEGEKDEAPMLYNGEKVGTGEMPQVDIAVDPIDGTTLLSRGQ
                     ANSIAVIAVSERGTMLDPGPCVYMEKVATGPEARDAIDIEKPIEDNIKAVAKAKGLDV
                     NEVTVAMLDRPRHDDFVTRARAIGARVRLFSDGDIATAIAAGRGFLGIDLLYGIGGTP
                     EGVTAAAALTCIGGSIQGRLWPRNDDERALAVEQGFDVDKPLTTKDLCSGEDIFFSAT
                     GITDGFLLEGVRYGKDRITTQTMIMRSRSGTLRTVIGEHDANKLRAISAEEPWRRH"
     gene            1187..1495
                     /locus_tag="VM841_04045"
     CDS             1187..1495
                     /locus_tag="VM841_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89389.1"
                     /translation="MRVVIAVADLMARSRVESAARAAGHDVFAVSAVPADGEPCDLLI
                     ADLDQPGALERLEAWRAAHPDVPVNGFAFHVNADTIERARAMGVRVKAHGSDPASLLD
                     "
     gene            complement(1492..2097)
                     /locus_tag="VM841_04050"
     CDS             complement(1492..2097)
                     /locus_tag="VM841_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL89390.1"
                     /translation="MVTRLPASARRAQLIEIGRVVFAERGYEAASVEEIAERAKVSKP
                     IVYEHFGGKEGLYAVIIDREMEIIVARIGEAIASGTPRQRLEHAALAFLTYVKDQPDG
                     FAVLTRDAPVTTAGGGISGLLNEVAERVGKIFIAEFKKAGYDAKTAPIYAHALVGMVT
                     FVGQWWTEVKKPSVETVAAHIAGLAWMGLRHLPKKPALDAR"
     gene            2175..3071
                     /locus_tag="VM841_04055"
     CDS             2175..3071
                     /locus_tag="VM841_04055"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HVL89391.1"
                     /translation="MAPSSTDLLRQLDPVVESMLSRHMASAQEWFPHEYVPWEQGRNF
                     TDEPWEPGDSTLSEVARIAMELNLLTEDNLPYYHLAIWEMFGGEGPWGEWARRWTAEE
                     GRHSIAMRDLLAVTRGVDPVALERGRMQHVSTGFYAGSMPDPLDGLVYVTLQELATRI
                     AHRNTGSVTQDPVADKLMARIAVDENLHYVFYRDVTAAALEADPSAAMQAIKRQVLGF
                     AMPGFELPSFREKAIKIAQAGIYDLRIHRDQVLMPVLMKQWQIDRIDGLDDDAARARD
                     DVMAFLDQLDQAATRYEEKRAG"
     gene            3204..4214
                     /locus_tag="VM841_04060"
     CDS             3204..4214
                     /locus_tag="VM841_04060"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HVL89392.1"
                     /translation="MPRRLLSLVALTVAFVLALPAASWACGGLVAGKHAEVLTKAVSL
                     AAWVDGVEHYITGFEFAGTADSFGYIIPLPAPPTEIRKGGDWTLERLEREAFPPPPVP
                     QADFAVAAGELADQSVEVLKAVKIDSLDIKVVRGGGRDVAAWARDHDFLMTPDTDDLL
                     AQYKANIFALAKFDKTEAQKKFVKGQGVVIDFEIPLPGPWIPLRILSLGKHEAEQINA
                     HLFMLTPERPVITPALDTLEGMTVVRDEWASDELIADLRSDTGTQWIPEKMWFTALEL
                     VAPSPTVTYDIAALGTGGITPVDPTGGDSRDGTWAWWLILTALGAAVLGISRRRASRP
                     AS"
     gene            complement(4297..5274)
                     /locus_tag="VM841_04065"
     CDS             complement(4297..5274)
                     /locus_tag="VM841_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89393.1"
                     /translation="MTPPMDRREFLAAGGAAFLGLVAAACSGGNGTTAPPNGGKSNTD
                     AKTIKELIAGKQNTVNVISVQPNLAREDDRLALALVPTGTDGGTVYKGGTARAWVATS
                     ETAPLQGPIDLEFHGDGFDRGVYIGRASFDTEGQWLVYVEATPEGSEQVQGGSTLVVG
                     NVPAAPGKDPQPVPGDKAISTPTPTLDDARGVDPICTRVNDAGKREPCTMHQISLDQA
                     LKNGKPTVLIIGTPAFCESRFCGPIVDQLMIVQKETADRANFVHIEQWADDKDAPATG
                     TLAPAAAAWRLAAEPVVYYIKPDSTIAEWTIGASDAREMRDLTKTTLLS"
     gene            5491..5928
                     /locus_tag="VM841_04070"
     CDS             5491..5928
                     /locus_tag="VM841_04070"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HVL89394.1"
                     /translation="MSKRYDEPADVELNSDAGIEAFWWRGRRYRVRRVLSRWREAGGW
                     WESAADTAQPWARGESKEILRVDAFPDANAPVSADPVSAEPVAATRTSGFAGVTTATA
                     LFAARSGGHHPAGRRRVRAPIAGTYELSRDLRKGTWTLLRIWD"
     gene            complement(6106..6178)
                     /locus_tag="VM841_04075"
                     /pseudo
     tRNA            complement(join(6106..6139,6143..6178))
                     /locus_tag="VM841_04075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:complement(6144..6146),aa:Ser,seq:tga)
     gene            6236..>6605
                     /locus_tag="VM841_04080"
     CDS             6236..>6605
                     /locus_tag="VM841_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVL89395.1"
                     /translation="MNQPAFAHLSVRTVYSLREGAIRPDELAARTAALGMEAVAITDT
                     NGLYGAVRFAQACAHYGIKPIYGTRLTVAGDDAAERFVLTLLAADNDGYTNLCRLLTA
                     AHNRGERGDPCATIEDVLDHA"
BASE COUNT          960 a         2241 c         2311 g         1093 t
ORIGIN      
        1 ccgacctgca gggccgtgcc ttcgtgcggc gccaggacga tcgcgtcgtc gccgctcatc
       61 agtggcggcg ccacggctct tcggccgaga tcgcgcgaag cttgttggca tcgtgctcgc
      121 cgatgacggt gcgcagcgtg cccgagcgcg agcgcatgat catcgtctgc gtcgtgatcc
      181 ggtccttgcc gtagcgcacg ccctccagca ggaagccgtc ggtgatgccg gtcgccgaga
      241 agaagatgtc ctcgcccgag cacaggtcct tggtcgtgag cggcttgtcc acgtcgaagc
      301 cttgctccac ggccaaggcg cgctcgtcgt cgttgcgcgg ccacagccgc ccttggatcg
      361 agccgccgat gcaggtcagc gccgcggcgg cggtgacccc ttcgggcgtg ccgccgatcc
      421 cgtacagcaa gtcgattccc aagaacccgc ggccggccgc gatcgcggtc gcgatgtcgc
      481 cgtcgctgaa caggcgcacg cgcgcgccga tcgcgcgggc gcgcgtcacg aagtcgtcgt
      541 ggcggggacg gtccagcatc gcgaccgtga cctcgttgac gtcgagaccc ttggccttgg
      601 cgacggcctt gatgttgtcc tcgatcggct tttcgatatc gatcgcgtcg cgcgcctcgg
      661 gaccggttgc gaccttctcc atgtagacgc acgggcccgg atccagcatg gtgccgcgct
      721 cgctgaccgc gatcaccgcg atcgagttcg cctggccgcg cgacagcagc gtcgtgccgt
      781 cgatcggatc caccgcgata tcgacctgcg gcatctcgcc ggtgccgacc ttctcgccgt
      841 tgtagagcat cggcgcctcg tccttctcgc cttcgccgat caccacgacg ccgtcgatgg
      901 cgacgctgtt gagcatcagc cgcatcgcgt ccacggccgc ggcgtcggcg gcgttcttgt
      961 cgccgcgtcc catccagtgg gcggcggcca gggcggcggc ttcggtcacg cggaccagct
     1021 cgagggccag gttccggtcg ggcttcgcgg gcgtcatggt cactcctgtc gtcggatgcg
     1081 gcgcgcttcg tgggcgccgc gaccccttca tttcaccatc cgtgcccgcc cgttgcacat
     1141 cggtccaccc ggaaatgggc aggtggtgcc gttagactcc ctcctgatgc gcgtcgtcat
     1201 cgcggtcgcc gacctgatgg cccgctcccg ggtcgaatcc gccgcgcgcg ccgccggcca
     1261 tgacgtcttc gccgtcagcg ccgtgccggc cgacggcgaa ccgtgcgatc tgctgatcgc
     1321 cgacctggat cagcccggtg cgctggagcg tctggaggcg tggcgcgcgg cccacccgga
     1381 cgtgcccgtc aacgggttcg cgttccacgt caacgcggac accatcgagc gcgcgcgcgc
     1441 gatgggagtt cgcgtcaagg cgcacggatc cgatccggcg tccctgctgg actaacgcgc
     1501 gtccagcgcc ggcttcttcg gcaggtgacg caggcccatc caggccaggc ccgcgatgtg
     1561 cgcggcgacg gtctcgacgg acggcttctt gacctcggtc caccactgtc cgacgaaggt
     1621 caccatgccg accagcgcgt gcgcgtagat cggcgcggtc ttggcgtcgt agccggcctt
     1681 tttgaactcg gcgatgaaga tcttgccgac gcgttcggcg acttcgttga gcagccccga
     1741 gatgccgccg cccgcggtgg tgacgggcgc gtcgcgggtc agcaccgcga agccgtccgg
     1801 ctggtccttc acgtaggtca ggaacgccag cgcggcgtgc tccaatcgct gccgcggcgt
     1861 gcccgacgcg atcgcctcgc cgatccgcgc gacgattatc tccatctcgc gatcgatgat
     1921 cacggcgtac aagccttctt tgccgccgaa gtgctcgtag acgatcggct tggagacctt
     1981 ggcccgttcg gcgatctcct cgaccgaggc ggcttcgtag ccgcgctcgg cgaagacgac
     2041 ccggccgatc tcgatcagct gggcgcggcg ggcggaggcg ggcagtcggg tgaccatcgg
     2101 ttgacaagga tactacgccg ccgtaaccta cgggaacgta agttacgcga gcgtagggag
     2161 agtgcggcat ggtcatggcc cccagttcca ccgatctgct ccgccagctt gatcccgtcg
     2221 tcgagtcgat gctgtcccgg cacatggcgt ccgcccagga atggttcccg cacgagtacg
     2281 tgccgtggga gcagggccgc aacttcaccg acgagccgtg ggagccgggg gacagcacgc
     2341 tgagcgaggt cgcgcgcatc gcgatggagc tgaacctgct gaccgaggac aaccttccct
     2401 actaccacct ggcgatctgg gagatgttcg gcggcgaagg gccgtggggc gaatgggcgc
     2461 gccgctggac ggccgaagaa ggccgccact cgatcgcgat gcgcgacctg ctggccgtta
     2521 cccgcggcgt ggacccggtc gcgctggaac gcggccggat gcagcacgtg tccacgggct
     2581 tctacgcggg ctcgatgccc gacccgctcg acgggctcgt ctacgtcacg ctgcaagagc
     2641 ttgcgacgcg catcgcgcac cgcaacaccg gctcggtcac gcaggatccg gtcgccgaca
     2701 agctgatggc gcgcatcgcc gtggacgaga acctgcacta cgtgttctat cgcgacgtca
     2761 cggcggcggc gctggaggcg gatccgtccg cggccatgca ggcgatcaag cggcaggtgc
     2821 tcggcttcgc catgccgggc ttcgagctgc cgtcgttccg cgagaaggcg atcaagatcg
     2881 cgcaggccgg catctacgat ctgcgaatcc accgcgacca agtgctgatg cccgtgctga
     2941 tgaagcagtg gcagatcgac cgcatcgatg ggctcgacga cgacgccgcc cgcgcgcgcg
     3001 acgatgtcat ggcgttcctg gaccagctcg accaggccgc cacccgctac gaggaaaagc
     3061 gcgccggctg agagctcggc ggtcgccggc gcgacggccc ggcaccggct cgagatcggc
     3121 ccggcgctgc gcaccgaatc ttcgggaacc ccggcggggg cgcgagcgtc gaactcctgt
     3181 aggccgacta cagggaggca gccatgccgc gtcgtttgct cagcctcgtc gcgctcaccg
     3241 tcgcgttcgt tctcgctctt cccgccgcgt cgtgggcgtg cggcgggctg gtcgccggca
     3301 agcacgcgga ggtgttgacc aaagcggtct ctctcgccgc atgggttgac ggcgtcgagc
     3361 actacatcac cggattcgag ttcgcgggca ccgcggactc cttcggctac atcatccccc
     3421 tgccggcgcc gcccaccgag atccgcaagg gaggcgattg gacgttggag cgactggagc
     3481 gcgaggcgtt cccgccgccg ccggtcccgc aggctgactt cgccgtagcc gccggcgagc
     3541 tcgcggatca atccgtcgag gtcctcaagg ccgtcaagat cgactcgctc gacatcaagg
     3601 tcgtgcgtgg cggcggacgc gacgtcgcgg cgtgggcgcg cgatcacgac ttcctgatga
     3661 cccccgacac cgacgatctg ctggcgcaat acaaggccaa tatcttcgcg ctggccaagt
     3721 tcgacaagac cgaggcgcag aagaagttcg tgaagggcca gggcgtcgtg atcgacttcg
     3781 agatcccgct gccgggcccg tggatcccgc tgcgcatcct gtcgctgggc aagcacgaag
     3841 ccgagcagat caacgcgcac ctgttcatgc tcacgcccga acgtcccgtg atcacgcccg
     3901 cgctggacac gctcgaaggt atgaccgtgg tgcgcgacga gtgggccagt gacgagctga
     3961 tcgccgatct gcgcagcgac accggcaccc agtggatccc cgagaagatg tggttcaccg
     4021 cgctcgagct ggtcgccccc tcgccgaccg tcacctacga catcgccgcg ctcggcaccg
     4081 gcggcatcac gccggtggac ccgaccggcg gggactcgcg ggacggcacc tgggcgtggt
     4141 ggctgatcct gaccgcgctc ggcgccgcgg tgctcgggat ctcgcgccgg cgggcgagcc
     4201 ggcccgcaag ctgagaggct ccgtttctgt gcgcatccgg cgccgcatag cgactccgga
     4261 tgcgcacgga acgctcgagc cggctgcgct ttcgcgctag gacagcaggg tcgtcttggt
     4321 gaggtcgcgc atctcgcgcg cgtccgaggc gccgatggtc cattcggcga tggtcgagtc
     4381 gggcttgatg tagtagacga cgggttcggc ggccaggcgc caagccgcgg cggcgggggc
     4441 gagcgtgccg gtcgcgggtg cgtccttgtc gtcggcccat tgctcgatgt gcacgaagtt
     4501 ggcccggtcg gccgtctcct tctgcacgat catcagctgg tcgacgatcg ggccgcagaa
     4561 gcgcgactcg cagaatgcgg gcgtgccgat gatcaggacg gtcggcttgc cgttcttgag
     4621 cgcttgatcc agcgagatct ggtgcatcgt gcagggctcg cgtttgcccg cgtcgttgac
     4681 gcgggtgcag atcggatcga cgccccgcgc atcgtccagc gtcggggtcg gcgtcgagat
     4741 cgccttgtct cccgggaccg gctgcgggtc cttgccgggg gcggcgggca cgttgccgac
     4801 gaccagggtc gagccgccct gcacctgctc ggatccctcg ggcgtcgcct cgacatagac
     4861 gagccactgg ccttcggtgt cgaagctggc acggccgatg tagacgccgc gatcgaagcc
     4921 gtcgccgtgg aattccagat cgatcgggcc ctgcagcggt gcggtctcgg aggtggcgac
     4981 ccaggcgcgg gcggtcccgc ccttgtagac ggtgccgccg tcggtgccgg tcgggacgag
     5041 cgcgagcgca agccggtcgt cctcgcgcgc caggttcggc tgcacggaga tgacgttgac
     5101 ggtgttctgc ttgccggcga tgagctcttt gatcgtcttg gcgtcggtgt tgcttttgcc
     5161 gccgttgggc ggggcggtgg tgccgttgcc gccgctgcac gcggccgcaa ccaagccgag
     5221 gaacgcggcg ccgccggccg ccagaaactc gcgtcgatcc atgggtgggg tcatgagagc
     5281 ccgatcctac cggcggcccg gagggcgtgc agacgatgct gtcggtggca tcggcgatcc
     5341 tgctcttgac ggaggcagcg gccgcggcgt accgtgtcct tctctctcga acatgtgttc
     5401 gaatgcttga ttcgggctcc gtacgggaga gggggaaggc ctccgccgcg ggttcgcagc
     5461 gagcgggtga ggccggaaag cctggggacg atgtcgaagc gatacgacga accggccgat
     5521 gtcgaactga atagcgacgc cgggatcgag gcgttctggt ggcggggccg ccgctatcgg
     5581 gtgcgccgcg tgctgtcgcg ctggcgcgag gccggcggct ggtgggagag cgccgccgac
     5641 accgcgcagc cgtgggcgcg tggcgagtcg aaggagatcc tgcgggtcga tgcattcccg
     5701 gatgcgaacg cgccggtgtc ggccgatccg gtgtcggccg agcccgtcgc cgcaacgcgg
     5761 acgagcgggt tcgcgggcgt caccaccgcg accgcgttgt tcgcggcgcg atcgggcgga
     5821 catcatccgg ccggccgccg ccgcgtgcgc gcacccatcg ccggcaccta tgagctgtcc
     5881 cgcgacctgc gcaaaggcac ctggacgctg ctgcgcatct gggactgatg acgatttcga
     5941 gcaaaaagaa aggagcaggc agtcctgctc ctttcatggc tcgcagtgtt gactctgact
     6001 cagcgatctc attgtggttc gtcttgctca gatggtcaag tgccaggtgg cgggacggtt
     6061 atctctggtt ggccgtcccg gcgatggtcc cctcagttga gggggtgggc tcggacggac
     6121 tcgaaccgtc atctcgaact gagtcagagt cgcgtgttgt cctttacact acgagcccac
     6181 cgccgcaatg cattctcggg cttcgcctcg cgaagtccat tccgggagaa gcgcgatgaa
     6241 ccagccggcc ttcgcgcatc tgtcggtccg gacggtctac tcgctccgcg agggcgcgat
     6301 ccggccggac gagctggcgg cgcgcacggc ggcgctcggc atggaggccg tcgcgatcac
     6361 cgatacgaac ggcctctacg gcgcggtgcg ctttgcgcaa gcctgcgcgc actacggcat
     6421 caaaccgatc tacggcacgc ggctcaccgt cgccggcgac gacgcggcgg agcggttcgt
     6481 gctgacgttg ctggccgccg acaacgacgg ctacaccaac ctgtgccggc tgctgaccgc
     6541 ggcgcacaac cgcggcgagc gcggcgaccc ctgcgcgacg atcgaggacg tgctcgacca
     6601 tgcac
//
LOCUS       DATLFO010000111         3352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_53191, whole genome shotgun sequence.
ACCESSION   DATLFO010000111 DATLFO010000000
VERSION     DATLFO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53191"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VM841_04085"
     CDS             <1..439
                     /locus_tag="VM841_04085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HVL89396.1"
                     /translation="ERRTVGRLDDAGRQRFHEEQMLAAEMLLLPREKIPPTVPALRRY
                     IEQVVDSGDLIVTDAAHRVAGLFRHPPREAEWRPVLRVVSRWAFGTLPARLRADYGIA
                     WNPVKEAALRASLSWLKTVRPLLPGTYREIEIARRAGEAAAAD"
     gene            724..1968
                     /locus_tag="VM841_04090"
     CDS             724..1968
                     /locus_tag="VM841_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89397.1"
                     /translation="MRGRFHESLTPSVHPPKIRATAQEGFATNSKDVFAALVSARRGT
                     DRAARDAERAELPEAQMLRRARAAADASPGDAHAEVAAKQAEARARTEALEWQVERLR
                     ALLAETKPVTFDYEATRPATEVPPFDAHGLDIEEPPPPAPPQAGEPGGLLGKLLPSAR
                     ARHEAAIAEAQAAFERAVEEHARREAERKQRLADALADYERTRSEAESGTAARNREVD
                     AVRAKIESGDPDAVAHYFAAILAAGDLPDGFPRRATVALDPGGRILVVDYELPGLEVV
                     PAVQAYRYDVALDSFEEVPRPAARRKDLYALVIASVALRSVAELFESDCGRVVDEIAF
                     NGYVHGIDRVSGEPVRPYLVSLRVTRDDLRRLDVTKTDPIVTMRTIQAAFSTNPADLA
                     AVKQVVGAKAVGTAHLATSPDV"
     gene            2016..>3352
                     /locus_tag="VM841_04095"
     CDS             2016..>3352
                     /locus_tag="VM841_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL89398.1"
                     /translation="MRRFRLPHPRLPRRISPVAARLFAVGALVFVASRIVAPVTLDVD
                     TSSLQVRAIPRLPGGHVVVPLGPLGRVTFTTHTSPLDFEANILIDPKAKALPDAEDID
                     VRSWRTTFILAKIPWLAAFGVLAVLLAVHGPPRHLAVSSAAGAATALVVAGAVAFAAV
                     GTFDLGALQNPRYTGPVRDVPRILQLVREISRDFPGTQRNIARAVEGLGRLRADLLAD
                     APAPPESTIRFLIAGDLQANPLGLLIAERLATEFEVNAILDAGDVTERGTAVEGELFA
                     AFSRISVPYVVVPGNHEDDAAIRRLSRIPNVRVITDDDDVVTIEGVTIVGAEDPNARS
                     IEVNPGNDLALERRPQICERLVDRVEQSGAQILIVHDPKIGECAEADAIERERPLLYV
                     WGHTHKPAFRRSGGVVGLSPGTSGANGIKSNNEAPYGFALVEMDASTHRPVSV"
BASE COUNT          482 a         1217 c         1162 g          491 t
ORIGIN      
        1 cgagcggcgc acggtcggac ggctcgacga cgccggccgg cagcgcttcc acgaggagca
       61 gatgctcgcc gccgagatgc tgctgctccc gcgcgagaag atcccgccga ccgttcccgc
      121 gctgcgccgc tacatcgagc aggttgtgga cagtggtgac ctgatcgtca cggacgcggc
      181 gcaccgcgtt gcggggctgt ttcgccatcc tccgcgcgag gcggaatggc ggccggtgct
      241 gcgggtggtg tcgcgatggg cgttcgggac cttgccggcc cggctgcgcg ccgactacgg
      301 catcgcatgg aacccggtca aagaagcggc gcttcgcgcg agcctgtcct ggctgaagac
      361 ggtccggccg ctgctgccgg gaacgtatcg cgagatcgag atcgcgcgcc gcgccggcga
      421 agcagccgca gcggattgaa gctgcgcttc cgggcgaccg agacgatctc gccgcattcc
      481 gggcagcacg cgcgtccggc gacggcgccg gcgatgaatg cggtgaagca ttcggggcat
      541 tcgcctcggc gcgcgcggcc ggcggccggg agcggccggc cgaccggcgt tatcgcgtga
      601 ccactggggc acgtgtcgcc ggcgggcgag atggggcggc ggcaaaggaa gcactgaacc
      661 atggcgatca actccccgcc tttctcggac gcacggccgg gattcttgaa gaagcggcgt
      721 gctatgcgtg gaaggttcca cgagtccttg actccctctg tccacccacc gaagattcgc
      781 gcaaccgcac aggaaggatt cgccacgaat tccaaggatg tcttcgccgc gctggtgtcc
      841 gcgcgccgcg gaaccgaccg tgccgcgcgc gacgcggaac gggccgagct tcccgaggcc
      901 cagatgcttc gccgagcccg cgccgcggcc gacgcctctc ctggggacgc gcatgccgag
      961 gtcgccgcca agcaggccga ggcccgcgct cgcaccgagg cgctcgagtg gcaggtggag
     1021 cgcttgcgcg cgctgctcgc cgagaccaag ccggtcacct tcgactacga ggccacgcga
     1081 cccgcaaccg aggtcccgcc gttcgacgcg cacggcctcg acatcgaaga gccgcctccc
     1141 ccggcgccgc cgcaggcggg cgagcccggc gggctgctgg gcaagctgct gccgtcggcg
     1201 cgcgcgcgcc acgaggccgc gatcgcggag gcgcaagccg ccttcgaacg cgccgtcgaa
     1261 gagcatgcgc gccgggaggc ggagcgaaag cagcgactcg ccgacgcact cgccgactac
     1321 gagcgaaccc gctcggaagc cgagtcgggc acggccgccc gtaaccgcga ggtcgacgcc
     1381 gtgcgcgcca agatcgagag cggcgatccc gacgcggtcg cgcactactt cgccgcgatc
     1441 ctggcggcgg gcgacctgcc cgacggcttc ccgcgccgcg cgaccgttgc gctcgacccc
     1501 ggcgggcgca tcctggtcgt ggactacgag cttcccggcc tcgaggtcgt gccggccgtg
     1561 caggcgtacc gatacgacgt cgccctcgac tccttcgagg aggtcccgcg gccggccgca
     1621 cggcgaaagg acctgtacgc gctcgtgatc gcttcggtcg cgctgcgctc ggtcgccgag
     1681 ctgttcgagt ccgattgcgg ccgggtggtg gacgagatcg ccttcaacgg ctatgtccac
     1741 ggcatcgacc gcgtcagcgg tgagccggtc cgtccctatc tggtcagcct tcgcgtcacc
     1801 cgcgacgatc tgcgccgcct cgacgtgacc aagaccgatc cgatcgtgac catgcgaacc
     1861 atccaagcgg ccttctcgac caatccggcc gacctggccg cggtcaagca ggtcgtcggc
     1921 gcgaaggccg tcggcaccgc gcatctggcg acgtcgcccg acgtctgacg gccctttcgc
     1981 tttcgcgcag gccgggattg gggtagacct ctggggtgcg acgcttccgc ctgcctcatc
     2041 ctcgccttcc gcgccggatc agcccggtcg cggcacgcct gttcgcggtg ggcgcgctcg
     2101 tgttcgtcgc ctcccggatc gtggcacccg tcacgctcga cgtcgatacc agctcgctgc
     2161 aggtacgggc gatcccgcgg ttgcccggcg gacacgtcgt ggttccgctc ggtccgctcg
     2221 gccgggtgac cttcacgact cacaccagcc cgctcgactt cgaagccaac atcttgatcg
     2281 atccgaaggc gaaggccttg cccgatgccg aggacatcga cgtgcgctcg tggcgcacga
     2341 ccttcatcct ggccaagatc ccgtggctcg cggccttcgg cgtgcttgcc gtgctgctgg
     2401 ccgtgcacgg ccctccgcgg catctcgcgg tctcgtcggc ggccggcgcg gccaccgcgc
     2461 tcgtcgtggc cggcgcggtc gctttcgcgg cggtcggcac cttcgacctg ggcgcgctgc
     2521 agaatccgcg ctacaccgga ccggtccgcg acgtgccacg catccttcag ctcgtgcgcg
     2581 agatcagccg cgactttccg ggaacgcagc gcaacattgc gcgcgccgtc gaagggctcg
     2641 gccggcttcg cgccgacctg ctcgccgacg cgccggcgcc gcccgagtcc acgatccgct
     2701 tcctgatcgc cggcgacctt caggccaacc cgctcgggct tctgatcgcg gagcgtctcg
     2761 ccaccgagtt cgaggtgaac gcgatcctgg acgcgggcga cgtcaccgag cgcggcacgg
     2821 ccgtggaagg cgagctcttc gccgcgttct cgcgcatctc cgttccctat gtggtcgtgc
     2881 ccggcaacca cgaggacgac gcggccattc gccgcctgtc gcgcatcccg aacgtgcgcg
     2941 tgatcaccga cgacgacgat gtcgtcacga tcgagggcgt gaccatcgtc ggcgccgagg
     3001 atccgaacgc ccgctcgatc gaggtcaacc ccggcaacga tctcgcgctg gagcggcggc
     3061 cgcagatctg cgagcgtctg gtcgatcggg tcgagcagag cggcgcgcag atcctgatcg
     3121 tgcacgaccc gaagataggc gagtgcgccg aggccgacgc gattgagcgg gagcggccgt
     3181 tgctgtacgt gtgggggcac acccacaagc cggccttccg ccgcagtggt ggcgtcgtcg
     3241 ggctttcgcc cggaacgagc ggcgccaacg gcatcaagag caacaacgaa gcgccgtacg
     3301 gcttcgcgct cgtcgagatg gacgcctcca cgcaccggcc ggtctcggta tg
//
LOCUS       DATLFO010000112         4030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_111763, whole genome shotgun sequence.
ACCESSION   DATLFO010000112 DATLFO010000000
VERSION     DATLFO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111763"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /gene="tsaD"
                     /locus_tag="VM841_04100"
     CDS             complement(<1..534)
                     /gene="tsaD"
                     /locus_tag="VM841_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVL89399.1"
                     /translation="MIVLGIETSCDETGVAVVEDFKVRSNVIASQTDLHARFGGVVPE
                     IASRAHLTNLSLITQQALSEAGVRLDDLDGIAVTHGPGLVGALLVGLSAAKSYAMALG
                     VPLIGVNHLEGHVETAFLEKPDLEPPVLALIVSGGHSMIAAMPGHSRFEILGQTIDDA
                     AGEAFDKIARFVGLGFPG"
     gene            complement(531..1076)
                     /gene="rimI"
                     /locus_tag="VM841_04105"
     CDS             complement(531..1076)
                     /gene="rimI"
                     /locus_tag="VM841_04105"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HVL89400.1"
                     /translation="MRAPADPPGVPLPVEITTMTRKHVRDVLAVERLVYPRPWSAALF
                     FSELAQKSSRLYLVAVIEGEVVGYGGLMCHLDEGHITTLAVHPDFQRHRVGSKILYLL
                     ASDARERQMRTLALEVRVANWPAQRLYSWFGFRPVGIRRNYYAETGEDALVMWIDDMQ
                     SEEIATRLARVRERIGPVKVA"
     gene            complement(1073..1840)
                     /gene="tsaB"
                     /locus_tag="VM841_04110"
     CDS             complement(1073..1840)
                     /gene="tsaB"
                     /locus_tag="VM841_04110"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVL89401.1"
                     /translation="MLVLGIETSTPVSSVAIGSEQGVVASMTLARGRGHEEFLAPAIR
                     YLCEQSGVSLDQLAGIAVGVGPGLFTGMRVGIATGKGLAQALSVPMVGLPSLDLLAFG
                     VRYTSRLICACIDARRKEVFSAFYRQVPGGVQRISDYHVEPPERLASELDYHGTDVLF
                     VGNGALVYRDTLKRGRAEFAAVSRAFPSANALVELALPRFTREETDSLHELEPLYLRK
                     ADVTIGWETRGVMGIPPGATARTMGGRPPAGPSKGNG"
     gene            complement(1841..2335)
                     /gene="tsaE"
                     /locus_tag="VM841_04115"
     CDS             complement(1841..2335)
                     /gene="tsaE"
                     /locus_tag="VM841_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVL89402.1"
                     /translation="MTRRLECRSRSPEDTRAIAAAVAAVFAPGDVVALSGDLGAGKTC
                     FVQGAAREMGVTEHVSSPTFVLVREYPSGRIPILHIDVYRLGNLQELVDLGYEEFLDP
                     KQIVLIEWGDAVGPLLPPDYLHIEFHATGDDERTIELIANGPGWAARLESLKATLGGF
                     MEDR"
     gene            complement(2346..2972)
                     /locus_tag="VM841_04120"
     CDS             complement(2346..2972)
                     /locus_tag="VM841_04120"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVL89403.1"
                     /translation="MTLEALARAASGCTKCALSQGRTQVVFGSGNPSGDLMFVGEAPG
                     FHEDKQGVPFVGAAGNLLNDLLGKIGLIREDVYIANVLKCRPPGNRDPLPNEIEACNP
                     WLLRQIDLIDPAVICTLGNFSTQLLLQRKIGITRVRGQQFTYRGRTLIPTFHPAAVLH
                     GGGRGGSQYQMLQQDLKLVRSVIDAARPEPDDPTAGDAGDDAEQLGLF"
     gene            complement(3004..>4030)
                     /locus_tag="VM841_04125"
     CDS             complement(3004..>4030)
                     /locus_tag="VM841_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL89404.1"
                     /translation="AGLAAGAAIERATVGRVRSRPDPEAREPFGSLRGTPVGPIKSFD
                     GTLIHVEECGSGPTVVLAHGYSLNLSLWHYQIRDLASRYRLVLFDQRGHGRSETAGEG
                     GWSLEALARDLDAVVEQCGGDGRVVIVGHSMGGMALLTYCKLFHEKLGPRVAGIVLAD
                     TTAADVMPGMVEFLPRRVTAAFQGVQEAALRAVTAFDRASVDKLRRRANDLSWLLVRS
                     MGFGPHASPSHVAFLERMLSETPVDTWMELLPAVTGVDVSATLDLIDVPALVIVGTRD
                     RLTPPGAAERMVAGMKRATLGRIAGAGHCPMLETPHTFNARLRAFLDSLAVHGGRGPL
                     DPPGAAP"
BASE COUNT          637 a         1405 c         1390 g          598 t
ORIGIN      
        1 gccgggaaac ccgaggccga cgaagcgggc gatcttgtcg aaggcctcgc cggcggcgtc
       61 gtcgatcgtc tgaccgagga tctcgaaccg cgaatggccg ggcatggccg cgatcattga
      121 atgcccgccc gagacgatca gcgccagcac cggcggctcc agatcgggct tttcgaggaa
      181 cgcggtctcg acgtggccct ccagatggtt gacgccgatc agcggtacgc ccaacgccat
      241 cgcgtaggac ttcgcggcgc tcaatcccac gagcagcgcg ccgacgagcc ctggcccgtg
      301 cgtcaccgcg atcccgtcca ggtcgtccag gcgcacgccc gcttcggaca gcgcctgctg
      361 cgtgatcagg ctcaggttgg tcaggtgggc gcgcgatgcg atctcgggaa cgacgccgcc
      421 gaagcgcgcg tgcaggtcgg tctgggaggc gatcacgttc gagcggacct tgaagtcttc
      481 gacgacggcg acgccggtct cgtcgcagga cgtttcgatc ccgagcacga tcatgcgacc
      541 ttcaccggcc cgatccgttc acgcacgcgt gccagacgcg tggcgatctc ctccgattgc
      601 atgtcgtcga tccacatcac gagcgcatcc tcaccggtct cggcgtagta gttgcggcgg
      661 atcccgaccg gccggaaccc gaaccatgaa tacaggcgct gcgcgggcca gttcgcgacg
      721 cgaacctcga gcgcgagcgt gcgcatctgc cgctcgcggg cgtcgctggc gagcaagtac
      781 aggatcttcg atccgacccg gtgccgctgg aaatccggat gcaccgccaa ggtggtgatg
      841 tgcccttcgt cgaggtggca catcagaccg ccgtagccca cgacctcacc ttcgatcacc
      901 gcgacgaggt agagccgcga cgatttctgc gccagctccg agaagaacag cgccgcgctc
      961 cacggccgcg gatacacgag ccgctccacg gcgagcacgt cccgcacgtg cttgcgcgtc
     1021 atggtcgtga tctcgaccgg aagcggcaca cccggcgggt cggccggcgc tctcatccgt
     1081 ttcctttcga cggtccggcg gggggacggc cgcccatcgt gcgggcggtc gctcccggcg
     1141 ggatccccat cacgccgcgg gtctcccatc cgatggtgac atcggccttt cgaaggtaca
     1201 acggctcgag ctcgtggagc gagtcggtct cttcacgcgt gaaccggggc aaagcgagct
     1261 cgacgagcgc gttggccgac gggaacgcgc gtgagacggc cgcgaactcg gcgcggcctc
     1321 gcttgagcgt gtcccggtac acgagcgcgc cgttgccgac gaacagcacg tccgtgccgt
     1381 gatagtcgag ctcgctcgcg agccgctccg gcggctcgac gtggtagtcg ctgatgcgct
     1441 gcacgccgcc gggcacctga cggtagaacg cgctgaagac ctccttgcgc cgggcgtcga
     1501 tgcaggcgca gatcaggcgc gaggtgtagc gaaccccgaa ggcgagcaga tccagcgacg
     1561 ggagcccgac catcggcacc gacaacgcct gggcgagccc cttgccggtc gcgatgccca
     1621 cgcgcatgcc ggtgaacagg ccgggaccga cgccgaccgc gatccccgcg agctggtcga
     1681 gcgacacgcc ggactgctcg cacagatagc ggatcgccgg cgcgaggaac tcctcgtggc
     1741 cgcgtccacg tgcgagcgtc atcgacgcga ccacgccttg ctcggatccg atcgcgaccg
     1801 aggacaccgg cgtggatgtc tcgattccca gcaccagcat ctatcggtcc tccatgaagc
     1861 cgcccagggt cgccttcaac gactccagcc gcgcggccca gcccggtccg ttcgcgatca
     1921 gctcgatcgt tcgttcgtcg tcgccggtcg cgtggaactc gatgtgcaga tagtcgggcg
     1981 gcaggagcgg gccgacggcg tcgccccatt caatgagcac gatctgtttg gggtcgagga
     2041 actcctcgta gcccaggtcc accagctctt gcaggttgcc gagccggtaa acgtcgatgt
     2101 gcaggatcgg gatccggccg ctcggatact cacggacgag cacgaacgtg gggctcgaaa
     2161 cgtgctcggt gacgcccatc tcgcgcgccg cgccctgcac gaagcaggtc ttgccggccc
     2221 ccagatcccc cgacagcgcc accacgtcac cgggcgcgaa cacggcggcc acggcagcgg
     2281 cgatcgcccg cgtgtcttcg ggcgagcggg agcggcattc cagacgtcgc gtcacggcgt
     2341 ctccttcaga acagcccgag ctgttcggcg tcgtcgcctg cgtcgccggc cgtcgggtcg
     2401 tcgggttcgg gccgtgcggc gtcgatcacg cttcgcacga gcttgaggtc ttgctgcagc
     2461 atctggtact ggctgccgcc ccgtcccccg ccgtgcagca cggcggccgg atggaacgtc
     2521 ggaatcagcg tgcgcccgcg ataggtgaac tgctggccgc ggacgcgtgt gatcccgatc
     2581 ttgcgttgca gcagaagctg ggtggagaag ttcccgagcg tgcagatgac cgccggatcg
     2641 atcagatcga tctgacgcag cagccacggg ttgcacgcct cgatctcgtt cgggagcgga
     2701 tcccggttgc ccggcggccg gcacttcagg acgttggcga tgtagacgtc ctcgcggatc
     2761 aacccgatct tgccgagcag atcgttgagc aggttgccgg cagcgcccac gaacggcacg
     2821 ccctgcttgt cctcgtggaa gccgggcgcc tcgccgacga acatcaggtc gcccgaaggg
     2881 ttgccggagc cgaagacgac ctgcgtgcgt ccttgcgaca gcgcgcattt ggtgcagccg
     2941 ctcgctgcgc gagccagcgc ttccagcgtc atcgatcctc cacggccgtc cggccgcgca
     3001 attctacggg gccgcgccgg gcggatccaa ggggccgcgg ccgccgtgca cggcaagcga
     3061 gtccaggaag gcgcggagcc gggcgttgaa ggtgtgcggg gtttcgagca tgggacagtg
     3121 gccggcaccg gcgatccgcc cgagcgtggc tcgtttcatg ccggcgacca tgcgctcggc
     3181 cgcgccgggc ggggtgagcc ggtcgcgagt gccgacgatg acgagcgccg gcacgtcgat
     3241 caaatcgagc gtcgcgctga cgtccacgcc ggtcacggcc ggcagcagct ccatccaggt
     3301 gtcgaccggc gtctcggaca gcattcgctc gaggaacgcc acgtgcgagg gcgaggcgtg
     3361 cgggccgaac cccatcgacc ggaccaggag ccacgacagg tcgtttgcgc ggcggcgaag
     3421 cttgtccacg ctcgcgcggt cgaaggcggt cacggcgcgc agcgcggcct cttggacccc
     3481 ttggaacgcg gcggtgacgc gccgcggcaa gaactcgacc atgccgggca tgacgtcggc
     3541 cgccgtggtg tcggcgagca cgatgcccgc gacgcgcggg ccgagcttct cgtggaagag
     3601 cttgcagtag gtcagcaggg ccattccgcc catcgagtgg cccacgatca cgacgcgtcc
     3661 gtcgccgcca cactgctcga cgaccgcgtc caggtcgcgg gcgagcgcct ccagcgacca
     3721 gccgccttcc ccggcggtct cgctgcggcc gtgaccgcgc tggtcgaata gcacgagccg
     3781 gtagcgcgag gcgaggtcgc ggatctggta gtgccacagc gacaggttga gcgagtagcc
     3841 gtgcgcaagc accacggtcg gtccgctgcc gcactcttcg acgtggatca gcgtgccgtc
     3901 gaacgacttg atcggcccca ccggcgtccc gcgcagcgat ccgaacggct cgcgcgcctc
     3961 gggatcggga cgcgaccgca cccggccgac cgtggcccgc tcgatggccg cgccggccgc
     4021 gagcccggcg
//
LOCUS       DATLFO010000113         7686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_103208, whole genome shotgun sequence.
ACCESSION   DATLFO010000113 DATLFO010000000
VERSION     DATLFO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103208"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VM841_04130"
     CDS             <1..455
                     /locus_tag="VM841_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVL89405.1"
                     /translation="MERPGPVALEHAKAMEVAGVARYLTRDRDGGRALVEEAAGRFDA
                     ISAPLRAEHARLWLSENQVRRRGRKRSTLPAGLTQREIEILAMVVRGRSNQEIADALT
                     LSFATVKTHVERILAKTGAGRRSELAVIAMRLGVLPGSAPPESPGSDP"
     gene            537..746
                     /locus_tag="VM841_04135"
     CDS             537..746
                     /locus_tag="VM841_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89406.1"
                     /translation="MGKLKRLLAVLSMAAAIVALGAGTAFAADDPLECLKELKICDRH
                     PVTQWVGGFLGGDPTIPSIPDVENP"
     gene            848..1042
                     /locus_tag="VM841_04140"
     CDS             848..1042
                     /locus_tag="VM841_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89407.1"
                     /translation="MKKMTLAFALCAAMLPGFAQADPPPYDPISICDIPKGECYPERL
                     PDETRDFACSLVARVGECKI"
     gene            complement(1403..1963)
                     /locus_tag="VM841_04145"
     CDS             complement(1403..1963)
                     /locus_tag="VM841_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89408.1"
                     /translation="MRRIACCVAVAVAVFVTGPLALAQRSDGEVGMEDNELHRGQTVS
                     MASKPFEAGTTLTWSTAPHPAGCHASGGCDPPTKIATTTADAQGRGTFRWTVPRNMEY
                     GTYEMIVTGRSRGEDARYTSQFDVVPGGESSTTGGQLPATGAGTTALFALGLMLVVAG
                     GLALTVPGGARRGPAEPACVKSRTRA"
     gene            complement(2156..2596)
                     /locus_tag="VM841_04150"
     CDS             complement(2156..2596)
                     /locus_tag="VM841_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89409.1"
                     /translation="MKRIHGMAVAGMVLAIALGLSGAQAHTSPTHGVEGTATITATSV
                     PVWVSIKSAPFPGTGGSGVMRIGPKSYDVTCAYVSSNFAGTVHMLRASGTEMTSPFSP
                     VYLLIHQGPGAILRVRESSTATTLPCGAPSAVPPSPATGSFTWV"
     gene            3136..5337
                     /locus_tag="VM841_04155"
     CDS             3136..5337
                     /locus_tag="VM841_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89410.1"
                     /translation="MASRPRGVVPVAAGLVPALLFGSIMVGARLPSGGRHQHATLGAL
                     DMSRGPAGGFGSLRDSFLAGIFGPRAGTTDGIANAVTRSVRRPDPLREAEPVVVSHAP
                     TNDDFARAYVVPSVPFTARTDTTTATREPGEPAGCAPVGETVWYRFRAASDVGLIADT
                     FGTGRPVTIGVFAGDSVDRLTQVGCATHAAGNAHVAFPAAAGTIYSVQIAPVAAGPLV
                     FDLRLQGVTQRIRESDDASSLGTTAIGTAPSPNGEWLMVGSIAEDNPSRQCIAFTWGM
                     PLLPAFACRPAVYVVERKTGRKTMVSVNSNGEAANDLIAAQSMSEDGRFVGLSGFATN
                     MVPGDTNTCHAYRVPGHCPDVFVHDRDADGNGIFDETRPGARTTIRVSVTTDGTQADD
                     WSAVSLLSRDGRYVTFMSQASNLVPNDTNGVGDIFVRDRDTDEDGIFDEPGAVRTTRV
                     SVSSSGEEARDTPPDPQGATQRIPTERGASILGASSNGHYVLFRSAAPNLVPGDTNGF
                     ADVFLRDVRAGTTTRVNVGPGGIQANGWTRMPNGFQWKVSDDGRFAVFGSDATNLVPG
                     DTNNAEDLFVRDLVAGTTERVTLAADGSQAIGEGPAIGQDAIFGGILAVASIGVWNVY
                     TLSEMNHTMTPDGRYVFFSSGARNLVPEDANDQRDIFVRDRVRGTTTLVSVSSAGRQG
                     NASSNLPIPTIDGRLLTFTSASTTLEDEAASVIPRFGAGAFVRTLPWAVSR"
     gene            5334..7217
                     /locus_tag="VM841_04160"
     CDS             5334..7217
                     /locus_tag="VM841_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL89411.1"
                     /translation="MTTSRPIARFGDLPLSWKLLLPSVGLIVVLGILGGFLIVRDLSA
                     RAHAQLDLELSRHLLEAQSLFRDRELSLIEAVNFASNIDGMADAVRARDGEAVARLLQ
                     SVAALKTNVELLTVTDPSGRSLVEFDRSEPGERPVRRDGRTWTEPFAGEAARSEAGAK
                     VAGFAGDGEDAVLAVAAPICSETGTCAPVGTAIAGMNAGRVAAEALGRLTSPLAERAG
                     LTVFDRSGRALSTAGPLAPGRPAAAAGPGASRVDERIGATRAAILYSPFTVGEQRLGT
                     IAVSIPVAPTEAAVRRTGLGLGLILLAAMIGITVIGVILSRLMMRRVRSLVETNRALG
                     RGNLSARARVQSNDEIGELAAHLNEMAEQLQAMYSTLESRVAERTEEVERLLRERTEF
                     FASVSHELRTPLAVILRHAEMLLDNAYRGNGRWRTDAGSALRDSGQQLSHLIDDILEL
                     AEVEAGRLDVEIEPVDVGAFLHEMRATIVPLARAAGVRATFHATDRALPPVAADPRRL
                     RQILLNLADNAIKYSPEGGSVDLSAAGRGGVVEIVVSDTGVGIPPEAGDQIFEPFHRV
                     KGVQTQRGQASSGLGLAIARRLAEAHGGTIGYVARDGGGTTFTVRMPVFAPAEAERDD
                     VDA"
     gene            7210..>7686
                     /locus_tag="VM841_04165"
     CDS             7210..>7686
                     /locus_tag="VM841_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89412.1"
                     /translation="MPSAAKRAVKGISVLLVDDHPLWRDTLRKVLERKGFATVVAEAA
                     DAGEALAAARASAPDVIVMDIQLPGASGIDATRDLVSERPDVKVLVLASSDNRAQVVA
                     AVEAGARGYLIKTAGSDDVRESVRRVHEGELVFPPALASVVIDRLRGGDDGPADASR"
BASE COUNT         1265 a         2663 c         2582 g         1176 t
ORIGIN      
        1 ggatggagcg gcccggtccc gtggcgctcg aacacgcaaa ggccatggag gtcgcgggcg
       61 tcgcccgata cctgacacgc gaccgggacg gtggccgcgc gctcgtcgag gaggcggccg
      121 ggcgcttcga tgccatctcc gccccgctgc gggccgaaca cgccaggctg tggctttccg
      181 aaaaccaagt tcggcggcgc ggccggaagc ggtccacgct gcccgccggg ctgacccaac
      241 gcgagatcga gatcctcgcc atggtcgtac gcggccggtc caaccaggag atcgccgacg
      301 cgctgaccct gagcttcgcg accgtcaaga cccacgtgga acggatcctg gccaagaccg
      361 gggcgggccg ccgctccgag ctggcggtga tcgccatgcg tctgggcgtc cttcccgggt
      421 ccgccccacc ggaaagcccc gggtccgacc cctaattcat acgccgatcg gccgacgttt
      481 ttgccccgcc cggcagctac gattcgtcaa tcacacaaag gaactcggag gatcgaatgg
      541 gcaagctcaa gagactgttg gcggtgcttt ccatggccgc cgccatcgtc gcgctcggtg
      601 ccggaaccgc gttcgcggcc gatgacccct tggagtgtct caaggaattg aaaatctgtg
      661 atcgtcaccc ggtcacgcag tgggtcggag gtttcctcgg cggcgatccg acgattccgt
      721 cgatccccga cgtcgagaac ccgtagcgaa tcgtcccgcc cgctcggatt agttcggccg
      781 ggcggcggac ggcaagacgc tcatcgcgtc ctagcctggg ggcatccgtg aacccaagga
      841 ggacgcgatg aagaagatga cgctcgcatt cgcattgtgt gcagcgatgc tcccgggctt
      901 cgcccaggcc gacccgccgc cctacgaccc gatcagcatc tgcgacatcc cgaagggaga
      961 gtgctacccg gagcggctgc ccgacgagac cagggatttc gcctgcagcc tcgtcgcgcg
     1021 cgtgggcgag tgcaagatct gaccaaactc gcgcgaccag ccggcacgaa acgttcagcg
     1081 aggccctcgc atcgagaggg cctcgctcgt gccgaggacg gggctttccg ccgcaaccgt
     1141 acccgcaagg tcgttggcgg cgacgacctc gtggacggcg tccgcggtga cacgttcgcg
     1201 agctcgtagc aggccgccaa ggccacgtgc aggtcgtcgt ccgccagcgc gtccacagtg
     1261 ggtgaaaccg ccggcatcgc gccgatgtta tgcgggcgag acctcaatcg atcaaagacg
     1321 aaggcgctca gggacccccg aagacaagga aacttcacag caccggtttg gtcgagatcg
     1381 cacctgccga aacctcgcct gtctacgccc gggttctgct tttcacgcaa gcaggttcgg
     1441 ccggccctcg ccgggccccg ccgggaaccg tcagggccaa ccctccggcg acaacgagca
     1501 tgaggccgag ggcgaagagc gcggtcgttc ctgcgccggt tgccggcaat tgcccgccgg
     1561 tcgtcgaact ttccccgccc ggaaccacat cgaattgtga cgtgtagcgg gcatcttcac
     1621 cacgcgacct gccggtcacg atcatctcgt aggtgccata ctccatgttc cgggggacgg
     1681 tccaccgaaa tgtgccccgc ccctgggcgt ctgcggttgt cgtcgcgatc ttcgtcggcg
     1741 gatcgcagcc gcccgaggcg tgacaacccg ccggatgcgg agcggtgctc caggtgagcg
     1801 tcgttccggc ttcgaacggc ttcgaggcca tggagaccgt ctggccgcga tgaagctcat
     1861 tatcttccat ccccacttca ccatcgctgc gctgcgcgag agccagcgga cccgtcacga
     1921 aaacggcaac ggcgacggca acgcagcaag cgatccggcg catcccgctc tccccgatcc
     1981 caacgaagtt tccttatcag gtgcgagtgt atagcggcat ccgcaaacga gatagagccg
     2041 cggcggcggg gacctcgagt cggatccctg agcgaagccg atgatcgccc gcggctgcag
     2101 acgatccggc gccgcccgtc agcgaacggg cagcgccgga agcgatcgag gcttgctaga
     2161 cccaggtgaa cgaccccgtg gccggcgacg gcgggaccgc cgagggggcg ccgcacggca
     2221 gcgtggtagc ggtcgagctt tcgcggaccc gcaagatggc gccgggacct tggtggatca
     2281 gcaggtaaac gggcgagaac ggcgaggtca tctcggtgcc gctcgcgcga agcatgtgga
     2341 ccgttccggc gaagttgctc gagacgtacg cgcacgtgac gtcgtaggat ttcggtccga
     2401 tccgcatcac tccgctgcct cccgttcccg gaaagggcgc gctcttgatc gacacccaaa
     2461 ccgggaccga ggtcgccgtg atcgtcgccg tgccttccac gccgtgggtc gggctcgtgt
     2521 gcgcctgtgc tccgctgagc cccagcgcga tcgccagcac catcccggcg accgccattc
     2581 cgtgaatccg cttcatgacc gctcctccct ctcggttgac ggccccacta tgacaggagc
     2641 agcgcaaacc gtctgtgccg aagtgcacac cgggccggcg caatcctgcc ggtcctttct
     2701 gcggattgag caaacggcgc tttccgccga tgccccgacg ccggtacgag ccgtaaatgc
     2761 cggcagttcg catcagccgc tccactcgtt tgcgcgagca gattcggccg cggcccatcg
     2821 tgagctcgcg ccacatcatc ggcgaaccgt tatcggcgcc tgccgatagt aggctgcacg
     2881 agacccggcc tggaaacacc gaccgtcatc gaagccggct tctccatcac cggcacgtgg
     2941 ccaatgcgaa ctgagcgcga ctttggcatg aagacgggca ccaacgtcat cctcgtctgc
     3001 cgcaagcgag accgcgccta gagcacgttc ttggcggatc ggccattgcc gaaatcgccg
     3061 tatccagcgt aacgtccgta actgcgagtc ttacggcatc gggtcgaatc gattcagagc
     3121 gcagggggga tcctgatggc aagccggccg cggggcgttg ttccggtcgc cgccggactg
     3181 gttccggcgt tgctcttcgg ctcgatcatg gtcggcgccc gtctgccgtc cggcggccgg
     3241 caccaacacg cgacgctcgg ggcgctcgac atgtcgcgcg ggccggccgg cggattcggt
     3301 tcgcttcggg attcgttcct ggcgggaatc ttcgggcccc gcgcaggcac cacggacggg
     3361 atcgccaacg cggtcacgcg atccgtccgc cggcccgatc cgctgcgcga agcggagccc
     3421 gtcgtcgtgt cgcacgcgcc gacgaacgac gacttcgctc gcgcctacgt cgttccgtcg
     3481 gttccgttca ccgcgcggac cgacaccacc accgcgacgc gcgaacccgg cgaacccgcg
     3541 ggctgcgcgc ccgtcggcga gaccgtctgg tatcgattcc gcgccgcatc ggacgtcggc
     3601 ctgatcgccg atacgttcgg gaccggacgg ccggtcacga tcggcgtgtt cgccggcgac
     3661 tcggtcgacc gattgacgca agtcggctgt gcgacgcacg cggccgggaa cgcgcacgtc
     3721 gcattccccg ccgccgcggg cacgatctac tcggttcaga tcgcgccggt cgcggccggc
     3781 ccgctggtgt tcgatctgcg tctgcaagga gtgacgcagc ggattcgcga atccgacgac
     3841 gcctcgtcat tggggacgac ggcgatcgga acggcgccct ccccgaacgg ggagtggctc
     3901 atggtcggtt cgatcgccga ggacaacccg agcaggcagt gcatcgcgtt cacctggggc
     3961 atgccgcttc ttccggcctt cgcgtgccgg cccgccgtct acgtcgtgga gcggaagacc
     4021 ggccggaaaa cgatggtgtc ggtgaactcg aacggcgagg cggccaacga cctgatcgcc
     4081 gcgcaaagca tgtccgagga cggccggttc gtcggcttgt cgggattcgc caccaacatg
     4141 gtgcccggcg acacgaacac gtgtcacgcg tatcgggtgc cgggccattg ccccgacgtg
     4201 ttcgtccacg accgcgacgc cgacgggaac ggcatcttcg acgagacccg ccccggggcg
     4261 cgcacgacga tccgggtttc ggttacgacc gacgggacgc aggccgacga ctggagcgcg
     4321 gtctcgctgc tctcgcgcga cggccgctac gtcacattca tgtcccaggc ctcgaatttg
     4381 gttccgaacg acaccaacgg cgtgggcgac atcttcgtgc gcgaccgcga caccgacgaa
     4441 gacgggatct tcgacgaacc cggcgcggtt cgcaccacgc gcgtttcggt gtcgtcgtcc
     4501 ggcgaggagg cccgcgacac gccccccgat ccgcaaggag ccacccagcg catcccgacc
     4561 gagcggggcg catccatcct gggcgcctcc agcaacggac actacgtgct gttccgttcg
     4621 gccgcgccca acctggtgcc gggcgacacg aacggcttcg ccgacgtgtt cttgcgagac
     4681 gttcgggccg ggacgacgac gcgcgtcaac gtgggacccg gcggcatcca ggcgaacggg
     4741 tggacgcgca tgccgaacgg gttccaatgg aaagtgtccg acgacggccg cttcgccgtc
     4801 ttcggatcgg acgcgaccaa tctggttccc ggcgacacga acaacgcgga ggatttattc
     4861 gtccgcgacc tggtggccgg cacgaccgaa cgggtcaccc tcgccgcaga cggctcacag
     4921 gcgatcgggg aaggacccgc gatcggacag gacgcgatct tcggcggaat cctggctgtg
     4981 gcatcgatcg gggtgtggaa cgtgtacaca ctcagcgaga tgaaccacac catgacgccg
     5041 gacggccggt acgtgttctt cagttcgggc gcacgcaatc tggttcccga agacgccaac
     5101 gatcagcgcg acatcttcgt acgcgaccgc gtgcgcggca cgacgacact ggtctcggtg
     5161 tcgagcgccg gacggcaagg aaacgcatcc agcaacctgc cgataccgac gatcgacggg
     5221 cgcctgctga cgttcacgtc cgcatcgacg acgttggaag acgaagccgc gagcgtgatc
     5281 ccacgattcg gtgccggcgc attcgtgcgc acgctcccgt gggcggtgag ccggtgacga
     5341 cgagccggcc gatcgcgcga ttcggggatc tgccgttgtc gtggaaactg ctgcttccgt
     5401 cggtcggatt gatcgtggtg ctggggatcc tcggcgggtt cctgatcgtc cgcgatctgt
     5461 cggcgcgggc acacgcccaa ctggacctcg aactgtcccg gcatttgctg gaggcgcaat
     5521 cgctgtttcg cgaccgcgaa ctgtcgctga tcgaggcggt caacttcgcg tcgaacatcg
     5581 acggcatggc cgatgccgtg cgcgcccgcg acggcgaggc cgtcgcgcgg ctcttgcagt
     5641 cggtcgccgc gctcaagacc aacgtggaac tgctgaccgt caccgacccg tcgggacgga
     5701 gcctcgtcga gttcgaccgg tccgaacccg gcgagcgccc cgtgcgacgc gacggacgaa
     5761 cctggaccga accgttcgcc ggcgaagcgg cacgcagcga agccggcgcg aaggtcgccg
     5821 gattcgccgg cgacggcgaa gacgccgtcc tggcggtcgc ggcgccgatc tgttcggaga
     5881 ccggcacgtg cgcaccggtc gggaccgcga tcgccgggat gaacgccgga cgcgtcgccg
     5941 ccgaagcgct cggccggctc acctcgccgc tcgccgaacg cgccggcctg accgtcttcg
     6001 accgatcggg gcgcgcgctc agcaccgccg gtcccctcgc tcccggccgc cccgccgcgg
     6061 ccgccggacc cggcgcgtcc cgcgtggacg agcgaatcgg cgcgacccgc gcggcgatcc
     6121 tttacagccc gttcacggtc ggcgaacagc gcctggggac gatcgccgtc tcgattccgg
     6181 tcgcaccgac cgaagccgcc gtccgccgca ccggtctggg tctggggctg atcctgctcg
     6241 cggcgatgat cggcatcacc gtgatcggcg tgatcctgtc ccgcctgatg atgcggcgcg
     6301 ttcgctcgct ggtcgaaacg aatcgcgcgc tgggacgcgg caatttgtcg gcccgggccc
     6361 gcgttcaatc caacgacgag atcggcgaac tcgccgcgca cttgaacgag atggccgagc
     6421 agttgcaggc gatgtattcg acgctcgaaa gccgcgtcgc cgaacgcacc gaagaagtcg
     6481 aacgattgct gcgcgagcgc accgaatttt tcgcgtcggt cagccacgaa ctgcgcacgc
     6541 cgctggccgt catcctgcgc cacgccgaga tgctgctcga caacgcgtac cgcggcaacg
     6601 gacgctggcg caccgacgcc ggatcggcgc tgcgcgactc gggccagcaa ctatcccacc
     6661 tgatcgacga catcttggaa ctggccgaag tggaagcggg acgcctcgac gtcgaaatcg
     6721 agccggtcga cgtcggggcg ttcttgcacg agatgcgagc gacgatcgtt ccgctcgcgc
     6781 gagccgccgg cgtccgcgcg accttccatg cgaccgaccg ggcgcttcca ccggtcgcgg
     6841 ccgacccgcg ccgtctccgc cagatcttgc tgaatctcgc cgacaacgcg atcaaatact
     6901 cgcccgaagg cggatcggtg gacctgtcgg cggcgggtcg cggcggcgtg gtcgagatcg
     6961 tcgtgtccga cacgggcgtc ggcatcccgc cggaggcggg cgaccagatc ttcgagccct
     7021 tccatcgcgt gaagggcgtg cagacgcaac gcggacaggc gtcgagcggg ctcggactgg
     7081 cgatcgcgcg gcgtctggcc gaagcgcacg gcgggaccat cggatacgtg gcacgcgacg
     7141 gcggcggcac gacgttcacg gtccggatgc cggtgttcgc ccccgccgaa gcggagcggg
     7201 acgacgtcga tgcctagcgc ggccaaacgc gccgtcaaag ggatctcggt ccttttggtc
     7261 gacgaccatc cgttgtggcg cgacacgctt cgcaaggtgc tcgaacggaa aggtttcgcg
     7321 accgtggtgg ccgaagcggc cgatgcgggt gaggcactcg ccgcagcgcg cgcgtcggcc
     7381 cccgacgtga tcgtgatgga catccagctg ccgggcgcca gcggcatcga cgcgactcgc
     7441 gacctcgtct cggaacggcc ggatgtcaag gtgctggtgc tcgcctcgtc ggacaaccgg
     7501 gcgcaggtcg tcgcggcggt cgaagcggga gcgcgcggct acctgatcaa gaccgccgga
     7561 tccgacgacg tccgcgagtc ggtgcggcgt gtgcacgaag gcgaactggt cttcccgccg
     7621 gcgctggcgt cggtcgtgat cgaccgtctg cgcggcggcg acgacggccc cgccgatgcc
     7681 tcgcgc
//
LOCUS       DATLFO010000114         5088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_53309, whole genome shotgun sequence.
ACCESSION   DATLFO010000114 DATLFO010000000
VERSION     DATLFO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53309"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1558
                     /locus_tag="VM841_04170"
     CDS             35..1558
                     /locus_tag="VM841_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HVL89413.1"
                     /translation="MTTVAPPAARPEAPKGPRSPLGVLHHGWRTLRTMRTAIILLFVL
                     AAAASIGSFVPQRPINELAVIRWKAARPEMGQLFERIGFFDVYGAGWFMAIYALLLVS
                     LVGCIIPRYRAFWRVVRARPKPGARGAHTFEGVASCGPDDAIAIAERALRKRRYRTAR
                     IDGGVAAERGYWREGGSLMFHTAFLILLIGVAYGKGWGFNGQVAIVEGERFTETHLAY
                     DSINEGSRFGERHRGFTLQVDDFNATFEDNGVPKEFVSRVKLFEGDTLVRDTDIRVNN
                     PLVHRGVSIFQLAWGWAPRIVVSDPGGRVVHDAPVIFLPEGNGWRGVVKLPLAKPEHI
                     GLEMFFFNDLRIGDDGQPRNASPLPNRPVVVYQEFRGDLNLSVSQSVYELDKRKLKPG
                     KIGGIPLDGDSALDDGYTISFPGLRQYTVFQVAADPGQGIALAGAALILVGLFPALYS
                     SRRRVWIRVRPSESAGGEPESRVEVSGLALQRKASFEEEFGAIVRDLGRQLGGGGAR"
     gene            1555..2535
                     /gene="ccsB"
                     /locus_tag="VM841_04175"
     CDS             1555..2535
                     /gene="ccsB"
                     /locus_tag="VM841_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HVL89414.1"
                     /translation="MSVAGLSDTLFFVALGLYVVATVLFFVAIRREPVGEARSAAGRV
                     AIGLTVTGAAIHLGMVVTRAIASARVPWGNMYEYSSVLVFLLVVAMLAVVWKRPALRA
                     AEPLAMLIGVVTMALARGLYAPAGPLQPALQSWWLKIHVISAIAASSIFSIAFVFTVL
                     YLLKDRAERRALFTGSTVGAAWMGPVAVEERGEAGFPADHLEADPDARDAESPAGRGL
                     IARLPKSSTLDAWSYRLTAVAFPIWTWAVIAGAIWAAKAWSRYWAWDPKETWAFITWV
                     LYAAYLHARATSGWRGRRAAVIGVAGFVSLWITYYVVNLWIVGLHSYAGT"
     gene            2580..4070
                     /locus_tag="VM841_04180"
     CDS             2580..4070
                     /locus_tag="VM841_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVL89415.1"
                     /translation="MKKREEMKARVDALRAKHVEPLLARFPRMLPTAPDSAARYALYS
                     ALVWFVIWILIAATTALKLVFPEFIDQFGFMSYGRMRQAETNVLNWGVLFMGAVGAAF
                     AIIPRVCQVRLWSERIGAQIVIGLNQVVLAGTVLVFLGRTQGISGLEWPWPVDIALVA
                     IMFGVLQVLMATVMRRTEKRINAPARHLIAAFHVFPITYAVANFSTPFFYGVKQTYFS
                     GFGAAGFLLAMSLVGVGSSLFVLPRATGNPIYSEKLATYGWVTMLFFLPWLGLTARIL
                     GPGQDWLETLGITLALASLVPAAFVATNLIATARAGGGKDPAVKFMVGAGVIWALALV
                     QLAAGSFRHPAAIVGATWWNESVRTAFLGAFGLWLVGLMYHLIPRIRGRALRAPAIVN
                     LHFWIGTAGVVIAWASLAVAGVVQGYLLRVGAETGGEIAAGEGWRQISIAVQPMLVGR
                     MAAGAFIFTGIVLLLYNVQRTLAEGPETEPEPVVPERAPATVGGQS"
     gene            4070..4561
                     /locus_tag="VM841_04185"
     CDS             4070..4561
                     /locus_tag="VM841_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HVL89416.1"
                     /translation="MRNERGAFNIGIVIGLTVLALAMTAVLGILMPLTDPAVGSEAAG
                     QPLSYTQEQVEGRKIYIREGCFTCHTRMVRDAAADEPYGPMSLPADYFNEAPALLGLD
                     RVGPDLTCIGDRQSDIDALVKHLRNPGSVHEGSTMPSYAFLSDRELRAVASYLTALTC
                     QEA"
     gene            4561..4767
                     /locus_tag="VM841_04190"
     CDS             4561..4767
                     /locus_tag="VM841_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89417.1"
                     /translation="MSTVRVEEIGGIRSADRGVPGLLIIFAVAVLSVALWYLLSFSST
                     DYHGSYPKPDVPAVSEPAGGGGGH"
     gene            4774..>5088
                     /locus_tag="VM841_04195"
     CDS             4774..>5088
                     /locus_tag="VM841_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL89418.1"
                     /translation="MKFGLGQFTLQLPPWDSRTPAELYDDTLELAAFAEDHGFSSFWL
                     AEHHGASDDYNPSLLPMLAAVAARTRTMQIGTGVLLAPLHDPVRLAEDAAVVDAISGG
                     RLN"
BASE COUNT          692 a         1727 c         1763 g          906 t
ORIGIN      
        1 cagggtcgtg aacttcgcgc cgccgatcta accgatgacc accgtcgcgc cgcccgccgc
       61 acgacccgag gccccgaaag gcccgcggtc gccgctgggc gtgctgcatc atggctggcg
      121 cacgcttcga acgatgcgca cggccatcat cctgctgttc gtgctcgccg ccgccgcctc
      181 gatcggctcg ttcgtgccgc agcggccgat caacgagctc gcggtgatcc gctggaaggc
      241 cgcgcggccg gagatgggcc agctcttcga gcggatcggc ttcttcgacg tctacggcgc
      301 gggctggttc atggccatct acgcgctgct gctcgtgtcg ctggtcggct gcatcatccc
      361 gcggtaccgg gcgttctggc gcgtggtgcg ggcccggccc aagccgggcg cccggggagc
      421 gcacacgttc gagggtgtgg catcctgcgg gccggacgac gcgatcgcga tcgccgagcg
      481 ggcgctgcgc aagcgccggt atcgcaccgc gcgcatcgac ggcggcgtcg cggccgagcg
      541 aggctattgg cgggaaggcg gctcgctgat gttccacacc gcgttcctga tcctgctgat
      601 cggggtcgcg tacggcaagg gctgggggtt caacgggcag gtcgcgatcg tggaaggcga
      661 gcggttcacc gagacgcacc tcgcctacga ctcgatcaac gaaggcagcc gcttcggcga
      721 gcgccaccgc ggcttcacgc tccaggtcga cgacttcaac gcgaccttcg aggacaacgg
      781 cgtgcccaag gagttcgtct ctcgcgtgaa gctgttcgag ggcgacacgc tcgtccgtga
      841 caccgacatc cgcgtgaaca accccctggt ccatcgtggc gtgagcatct tccagctcgc
      901 gtggggatgg gcgccgcgca tcgtcgtgtc cgacccgggc ggacgcgtcg tccatgacgc
      961 gccggttatc ttcttgcccg agggaaacgg ctggcggggc gtagtcaagc tgccgctcgc
     1021 caagcccgag cacatcggtc tggagatgtt cttcttcaac gatctgcgga tcggagacga
     1081 cggccagccg cgcaacgctt cgccgctccc caatcgcccg gtcgtggtct atcaggagtt
     1141 tcggggagac ctgaaccttt cggtttcgca gtcggtgtac gagctggaca agcgaaagct
     1201 caagccggga aagatcggag gcatcccgct ggacggtgac tccgcgctcg acgacggata
     1261 cacgatctcg ttccccggtc ttcgccagta cacggtgttc caggtcgcgg cggatccggg
     1321 ccagggcatc gcgctcgcgg gcgccgcgct catcttggtg ggcctgttcc ccgccttgta
     1381 tagttcgcgc cggcgcgtct ggattcgcgt gcgaccatcc gagtccgccg gcggcgagcc
     1441 ggagtcgcgc gtcgaggtct cgggcctggc gctgcagcgc aaagcatcat tcgaggagga
     1501 gttcggcgcg atcgtgcgcg atctaggcag gcaactcggc gggggtggag ctcgatgagc
     1561 gtcgccggac tctccgacac cctcttcttc gtcgcgctcg gcctgtacgt cgtggcgacg
     1621 gtcctgttct tcgtggcgat caggcgcgag cccgtcgggg aggcgcgcag cgcggccggc
     1681 cgggtcgcga tcgggctgac cgtgaccggc gccgcgatcc acctgggcat ggtcgtgaca
     1741 cgcgcgatcg cgtccgcccg cgttccctgg ggcaacatgt acgagtactc ttcggtgctc
     1801 gtcttcctgc tcgtggtcgc gatgctcgcg gtcgtgtgga agcggcccgc gctgcgggcc
     1861 gcggagccgc tcgcgatgct gatcggcgtg gtcacgatgg cgctggcgcg cggtctctac
     1921 gcgcccgccg ggccgcttca gccggcgctg cagtcatggt ggctcaagat ccacgtgatc
     1981 tcggcgatcg ccgcctcgtc gatcttctcg atcgcgttcg tcttcaccgt cctgtatctg
     2041 ctcaaggacc gcgcggagcg acgagccctg ttcaccggca gcaccgtcgg cgcggcctgg
     2101 atgggtccgg tcgccgtgga ggagcgcggc gaggcgggct ttccggcgga ccatctcgaa
     2161 gccgatcccg acgcgcgcga cgccgagtcc ccggcgggac gcggcctgat cgcgcgactc
     2221 cccaagtcgt ccacgctcga cgcgtggtcc taccgcctca cggcggtggc cttccccatc
     2281 tggacgtggg ccgtgatcgc cggcgcgatc tgggccgcga aggcgtggag ccggtactgg
     2341 gcctgggacc cgaaggagac gtgggcgttc atcacgtggg tcctgtacgc cgcgtacctg
     2401 cacgcccgtg cgacctcggg atggcgcggc cgccgcgccg ccgtcatcgg cgtggccggc
     2461 ttcgtctcgc tgtggatcac gtactacgtc gtcaaccttt ggatcgtcgg ccttcactcg
     2521 tacgcgggga cctaatgccc aacccaccgg cacgtctgcc aacaccccgc gaggggatca
     2581 tgaagaagcg cgaggaaatg aaagctcgcg tggacgccct tcgcgccaag cacgtggaac
     2641 cgctgctcgc acggtttccc cgaatgcttc cgaccgcacc ggactccgcc gcgcgctacg
     2701 cgctgtactc ggcgctggtc tggttcgtga tctggatcct gatcgcggcg accaccgcgc
     2761 tgaagctggt gttccccgag ttcatcgatc agttcggatt catgtcctac ggccgcatgc
     2821 gccaggccga gacgaacgtc ctgaactggg gcgtgctgtt catgggagcc gtcggcgccg
     2881 cgttcgccat cattccccgt gtgtgccagg tgcgtctgtg gagcgagcgc atcggggccc
     2941 agatcgtcat cgggctcaac caggtcgtgc tcgccgggac cgtgctcgtc ttcctcggcc
     3001 gcacccaggg gatctcgggg ttggagtggc cgtggccggt ggacatcgcg ctagtggcga
     3061 tcatgttcgg cgtgctgcag gtgctcatgg ccacggtgat gcgccgcacc gagaagcgaa
     3121 tcaacgcgcc ggcccggcac ctgatcgccg cgttccacgt ctttccgatc acctacgccg
     3181 tcgcgaactt ctccacgccg ttcttctacg gcgtgaagca gacgtacttc agcgggttcg
     3241 gcgcggccgg cttcctgctg gcgatgtcgc tggtcggcgt cggctcgtcg ctgttcgtgc
     3301 ttccgcgcgc gaccgggaac ccgatctact ccgagaagct cgccacgtac ggctgggtga
     3361 cgatgctgtt cttccttccg tggctcgggc tcacggctcg catcctcggc ccggggcagg
     3421 attggctcga gacgctcggg atcacgctgg cgctcgcgtc gctcgtgcct gctgcgttcg
     3481 tggccacgaa cctgatcgcg accgcgcgcg ccggcggcgg caaggacccg gcggtgaagt
     3541 tcatggtcgg cgccggcgtc atctgggctc ttgcgctggt gcaactggct gccggatcgt
     3601 tccggcatcc cgcggcgatc gtcggagcga cctggtggaa cgagagcgtt cgaaccgcgt
     3661 tcctcggggc cttcggcctg tggctcgtcg gtctgatgta tcacctgatc ccgcggatcc
     3721 gcgggcgcgc gctgcgcgcc ccggccatcg tcaacctgca cttctggatc ggcacggcgg
     3781 gtgtcgtgat cgcctgggcg tccctcgcgg tcgccggcgt ggtgcagggc tacctgctgc
     3841 gggtcggcgc cgaaaccggt ggcgagatcg cggccggcga aggatggcgt cagatctcga
     3901 tcgccgtgca gcccatgctg gtcggccgca tggcggcggg agcgttcatc ttcacgggca
     3961 tcgtgctgct gctctacaac gtgcagcgca cgctggccga agggccggag acagagcccg
     4021 agccggttgt gcccgagcgg gcgccggcga cggtgggggg acaaagctga tgcgcaatga
     4081 acgtggagcc ttcaacatcg ggatcgtgat cgggctcacg gtgctcgcgc tcgcgatgac
     4141 ggcggtgctc ggcatcctca tgccgctgac cgatccggcg gtgggcagcg aggcggccgg
     4201 ccagccgctg tcctacactc aggagcaggt cgagggacgc aagatctaca tccgcgaggg
     4261 ctgcttcacc tgccataccc gcatggtgcg cgacgcggca gcggacgagc cctacgggcc
     4321 gatgtcgttg cctgcggact acttcaacga ggcgcccgcg ctgctcgggc tcgatcgcgt
     4381 gggtcccgat ctgacctgca tcggggaccg gcagagcgac atcgacgcgc tggtgaagca
     4441 tctgcgcaat cccggctcgg tgcacgaggg atcaacgatg ccctcgtatg cgttcctttc
     4501 cgatcgtgag cttcgcgccg tcgcctcgta cctgaccgcg ttgacttgcc aggaggcctg
     4561 atgagcaccg ttcgtgtcga agaaatcgga ggcatccgct cggccgatcg tggcgtgccc
     4621 ggcctgctga tcatcttcgc ggtggcggtg ctctcggttg cgctgtggta tctgctgtcg
     4681 ttctcgtcca cggactatca cggctcctac ccgaagccgg acgttccggc cgtgagcgag
     4741 cccgcgggcg gcggcggcgg tcactagcct ccgatgaagt tcgggctggg gcagttcacg
     4801 ctgcaactcc cgccctggga ctcgcgcacg ccggcggagc tctacgacga cacgctcgag
     4861 ctcgctgcgt tcgccgaaga tcacggcttc tcctcgttct ggctggccga acatcacggc
     4921 gccagcgacg actacaaccc gtccctgctg ccgatgcttg cggccgtcgc ggcccgcacg
     4981 cgcacgatgc agatcggcac gggcgtgctg ctcgcgccgc tgcacgatcc ggtccggctc
     5041 gccgaggacg cggccgtcgt ggacgcgatc tcgggtgggc gcctcaac
//
LOCUS       DATLFO010000115         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_23137, whole genome shotgun sequence.
ACCESSION   DATLFO010000115 DATLFO010000000
VERSION     DATLFO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23137"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VM841_04200"
     CDS             complement(<1..661)
                     /locus_tag="VM841_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL89419.1"
                     /translation="MSGDVIRVREEIDKAIEGKTVLEFFERNTALFGDRPALRRQTES
                     GWETVTWREYGERAVEIGAGLRALGVKHGDFVAIMARNVPEHVIADAGALHAAATPVS
                     IYNTLAPEQIAYIAKDCKAKVAVLENREFAKRWAEIKDQLPALTAVVLIDDAEDFRYL
                     GNVLSWDELIAKGRDALSKTPDLVKDAIAEITPDDPATIVYTSGTTGPPKGVEITHRN
                     VR"
     gene            868..1266
                     /locus_tag="VM841_04205"
     CDS             868..1266
                     /locus_tag="VM841_04205"
                     /inference="COORDINATES: protein motif:HMM:NF039659.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HVL89420.1"
                     /translation="MAGARPQQEEIARTPRAPIDQTSREINARQTLPEDEKPQAAVPK
                     KNRPGSHPKRDQDKPAFLTSKIELLRERRGDQQVTFADVCDHMNDYMDRNPEDERTIQ
                     RLALFLAKAEDADHDHAPPADGGLKEPIES"
     gene            complement(1474..2115)
                     /locus_tag="VM841_04210"
     CDS             complement(1474..2115)
                     /locus_tag="VM841_04210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVL89421.1"
                     /translation="MKRVLTTGAILAAMVMTSFVIAPQPVATATAGSPAPWSAPGSGA
                     VAVDPAPVGAAAGSNAASPPAPGPAALPARPVAARLSKIPAAGKMIEISLATQTLTAW
                     ENGRAVRSFKVSTGRAGYRTPTGTFKVRAKGIKWWSRKWQVWMPYAMNFYGNYNLHSL
                     PYSNPRKLIGASRLGRPDSHGCVRIGPDNARWLYGWTPKGTPVWIHEKSYRGP"
BASE COUNT          396 a          768 c          788 g          372 t
ORIGIN      
        1 agcgcacgtt gcggtgcgtg atctcgacgc ccttgggcgg cccggtcgtg cctgacgtgt
       61 agacgatcgt cgcgggatcg tcgggagtga tctcagcgat cgcgtccttg accagatcgg
      121 gcgtcttcga cagcgcgtca cggcctttgg cgatcagctc gtcccacgac agcacgttgc
      181 ccagataccg gaagtcttcg gcgtcgtcga tcagcacgac ggccgtgagc gccgggagct
      241 gatctttgat ctcggcccag cgcttcgcga actcgcggtt ctccaagacc gcgaccttcg
      301 ccttgcagtc cttggcgatg taggcgatct gctcgggagc gagcgtgttg tagatcgaga
      361 ccggcgtcgc ggccgcgtgc agcgcgccgg cgtcggcgat gacgtgctcg ggcacgttgc
      421 gagccatgat cgcgacgaag tcgccgtgct tgacgcccag ggcgcgaagg cccgcgccga
      481 tctcgacggc acgctcgccg tactcccgcc aggtgaccgt ctcccagccg ctctcggtct
      541 gacgccgcag cgcggggcgg tcgccgaaca gggccgtgtt gcgttcgaag aactcgagca
      601 ccgtcttgcc ttcaatcgcc ttgtcgatct cctcgcgaac cctgatcacg tcgccgctca
      661 tcacgatccc tccttggtgt cttcgcagga tatcgcgtga cgctcagcgg gaggcacctc
      721 gaggccggtc gcgactccgg cggctgcggc aaagcgcagc gcgatcgaaa tggcaccatt
      781 actcccttcc cccgtgcccg tcgaagccgg ctccggcgtg agccgcgccc gccgcgggta
      841 ttcagtcagc acgacgaaag gagacccatg gccggagcac gtccacagca agaggagatc
      901 gcccgcacgc cgcgggctcc catcgaccag acgagccgcg agatcaacgc acggcagacg
      961 cttcccgagg acgaaaagcc ccaggccgcc gtcccgaaga agaatcgacc cggctcgcat
     1021 cccaagcgtg accaagacaa gcccgcgttc ctgacgagca agatcgagct gctgcgcgag
     1081 cggcgcggcg accagcaggt caccttcgcc gacgtgtgcg accacatgaa cgactacatg
     1141 gatcggaacc ccgaggacga aaggacgatc cagcggctcg cgctgttcct ggccaaggcc
     1201 gaggacgccg accacgacca tgcgccgccg gcggacggcg gcttgaaaga gccgatcgaa
     1261 agctaacctg gggagatccg cccctctcgc gacgaggcgg ttgccgacgg cccgcgccgg
     1321 ctgttggtca tcgactggga cgcactcgtc aattttcttg acgagtgggt cgtgctgccg
     1381 gccactgctg ccggccactg ctgcgggcct catggacatc gcagcggccg ccggcgacgg
     1441 cttccggcga ggccggcgca tcgggcaatg tgatcatggc ccgcggtacg acttttcgtg
     1501 gatccagacg ggcgtgccct tgggggtcca tccgtagagc cacctcgcgt tgtccggccc
     1561 gatgcggacg catccatgcg agtcgggacg ccccagcctg ctcgcgccga tcagtttgcg
     1621 cgggttgcta tagggcaggg agtgcaggtt gtagttgcca tagaagttca tcgcgtaagg
     1681 catccaaacc tgccacttgc gcgaccacca cttgatgccc ttggcgcgta ccttgaatgt
     1741 gcccgtgggc gtgcggtagc ccgcgcgtcc ggtggacacc ttgaagctcc tgaccgcccg
     1801 gccgttctcc catgcggtga gcgtctgtgt ggcgagcgag atctcgatca tcttgcccgc
     1861 ggccggaatc ttcgacaggc gagcggcgac gggcctcgcg gggagcgccg ccggtccggg
     1921 ggccggaggc gacgcggcat tcgagccggc cgcggcaccg acgggcgcgg ggtcgaccgc
     1981 caccgcgccc gatccgggag ccgaccacgg tgccggcgag ccggcagtgg ccgtggccac
     2041 gggctgagga gcgatcacga acgaggtcat gaccatggcg gcgagaatgg ctccggtggt
     2101 cagcacacgc ttcacgggga aacctcctcg gtctcgatta agacaagcat caagaaacgt
     2161 cggccggaca aagccgttgc ttgaggggca aatggatcgt atgcctcata cccgaaacag
     2221 ggcaaggaca atcaaccgcg gtggggatga tcgccgtggg gatgatcgcc gtggggatga
     2281 tcgccgtggg gatgatcgcc gtggggatga tcgccgtggg gatg
//
LOCUS       DATLFO010000116         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_92652, whole genome shotgun sequence.
ACCESSION   DATLFO010000116 DATLFO010000000
VERSION     DATLFO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92652"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VM841_04215"
     CDS             <1..244
                     /locus_tag="VM841_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89422.1"
                     /translation="SGGSAFRLLSVVSDELDEPVGPHAGDIHEFRTGTSDTSGLLRAQ
                     RSKPPADRIYTLEYEGADLAGNMAGCTTTVAVGPSR"
     gene            604..1398
                     /locus_tag="VM841_04220"
     CDS             604..1398
                     /locus_tag="VM841_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89423.1"
                     /translation="MLDVRGIEVVYNDVVLALRGVSIHVPEGAIVAVLGANGAGKTTT
                     LRAITGLLDVHDAEITKGTVTFDSERIDKLDASAIVRRGIAQVLEGRRIFSELTVDEN
                     LKAGSFTRRDGAGIKQTYDWVMSLFPILGERRKQVAGYLSGGEQQMLAIARAMMASPK
                     LLILDEPSLGLAPLAVQTIRDTIVQINRSGTSILLVEQNAAMALSIATHGFVLENGKI
                     VMDKPAAELLEDEDVREFYLGLGGEGGGRRSYRDVKHYRRKKRWLS"
     gene            1395..2183
                     /locus_tag="VM841_04225"
     CDS             1395..2183
                     /locus_tag="VM841_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89424.1"
                     /translation="MTEPLLGFRGATLRFSGVTALSNVSFDVRPGELFAIIGPNGAGK
                     TSIFNVLSRVYRPQEGSVRFDGQDLLSMKRHEVARAGIARTFQNIELFPFLTVIDNLM
                     LGRHHLMSTGVAAGALWAGRAKRAEMQHREAAERVIEFLEIERYRNHMVTALPYGLQK
                     RVEMGRALAMEPKLLLLDEPVAGMNVEETEDTARFILDVKEELGIAQILVEHDMGLVM
                     DIADRIMVLDFGVKIAEGVPSDVQRDPAVIAAYLGEEQSAGAAP"
BASE COUNT          394 a          698 c          757 g          349 t
ORIGIN      
        1 ctccggcggc tcggcgttcc ggttgctgtc cgtcgtatcc gacgagctcg acgagcccgt
       61 cgggccgcac gcgggcgata tccacgagtt ccggacgggc acgtcggata ccagcggtct
      121 gcttcgggct cagcgcagca agccaccggc agaccgcata tacacgttgg aatacgaggg
      181 cgccgacctc gccggaaata tggcgggctg caccacgacg gtcgccgtcg gaccgtcacg
      241 ctgacccggt cgcgcgggcc cggcacccgc cgggcccgcg cgttccgacc gaagccggcg
      301 gggtgaagcc acgcccgaga gcggctgtcg acgcgacatg atcgcggtga tacgagcgtc
      361 tgcgtggtct cgctcagccc ggccccatga gcagcgcctc ccggcggatc cgtcacccgc
      421 gacatggcga tttttctgtc gccgagcgcg gttcgttggg gaaggcctct ctgacggcga
      481 gggatccccc ggtcagggca aacaatgacg ccgaaattcg ctcagcgcgg cacacatagc
      541 tctccccgca ccctggcgtc agggacgtaa agatggtatg tcctggaagc aggaggcggg
      601 tgcgtgctcg acgtccgagg aatcgaagtc gtctacaacg atgttgtcct tgcgttgcgg
      661 ggcgtttcca tccatgtgcc cgaaggggcc atcgtggcgg tcttgggggc caacggggcg
      721 ggtaagacca ccacgctgcg cgcgattacc ggcctgttgg acgtgcacga tgccgagatc
      781 accaaaggca ccgtgacctt cgacagcgag cgcatcgaca agctggatgc cagcgcgatc
      841 gtgcggcgcg gcatcgcgca ggtgctggaa ggccggcgca tcttcagcga gctaaccgtg
      901 gacgagaacc tgaaagccgg ctcgttcacc cgccgagacg gcgcagggat caaacaaacc
      961 tacgactggg taatgtcgct gtttccgatc ctgggcgagc ggcgcaagca ggtcgccggg
     1021 tatctgtccg gcggcgagca gcagatgctg gcgatcgctc gcgcgatgat ggcctcgccg
     1081 aaactgttga tcttggacga gccgtcgctg gggctggcgc cgctggcggt tcagacgatc
     1141 cgcgacacga tcgtgcagat caaccgctcg ggcacctcga ttctgctggt cgaacagaac
     1201 gcggccatgg ccctgtcgat cgccacccac ggcttcgtgt tggagaacgg caaaatcgtg
     1261 atggacaagc ccgcggccga gctgctggaa gacgaggacg tgcgcgagtt ctatctgggc
     1321 ctgggcggcg aaggcggcgg acgcaggtcc taccgcgacg tcaagcacta ccggcgcaag
     1381 aagcggtggt tgtcgtgacc gagccactgc tcggcttccg cggcgccaca ctccggttca
     1441 gcggcgtgac ggcgctgtcg aacgtgtcgt tcgacgtccg gccgggcgag ttgttcgcga
     1501 tcatcggccc caacggcgca gggaagacct cgatcttcaa cgtgctgtcg cgcgtctacc
     1561 ggccgcagga aggcagcgtg cgtttcgacg gacaagatct gctgtcgatg aagcggcacg
     1621 aggttgcacg cgccggcatc gcccgcacct tccagaacat cgagctgttc ccgttcctga
     1681 ccgtgatcga caacttgatg ctgggccggc accacctgat gtccaccggc gtcgcggccg
     1741 gcgcgttgtg ggcgggccgc gcgaagcgcg ccgagatgca gcaccgcgaa gcggccgagc
     1801 gggtgatcga gttcctcgag atcgagcgct accgcaacca tatggtgacc gcgctgccct
     1861 acggcctgca gaagcgcgtc gagatgggac gcgccctggc gatggagccg aagctgctgc
     1921 tgctggacga gccggtcgcc ggcatgaacg tcgaagagac cgaggacacc gcacgcttca
     1981 tcctggacgt caaggaggag ctggggatcg cgcagatcct ggtcgagcac gacatgggac
     2041 tggtgatgga catcgccgac cgcatcatgg tgctggattt cggcgtcaag atcgccgaag
     2101 gtgtgccgtc ggatgtacaa cgggaccccg ccgtgatcgc cgcgtatctc ggcgaagagc
     2161 aaagcgcagg agccgcccca tgagcacgac acagttgg
//
LOCUS       DATLFO010000117        19039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_61739, whole genome shotgun sequence.
ACCESSION   DATLFO010000117 DATLFO010000000
VERSION     DATLFO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61739"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="VM841_04230"
     CDS             complement(<1..1963)
                     /locus_tag="VM841_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVL89425.1"
                     /translation="MKPRWIVAAAVIAVLGVAVPPAPAATRRPSTWIPNTDTSWPMSP
                     GGDNFLTGYAPQETPPPFIGCRGTACRKTGDADGPWLEWVTKISDLPTGAFPTAAIVA
                     HDGLLYVGGGATNSFLALDMDTGLPVWRFSPDPRTDGVTAQYPSSNAPSIHNGLIYTT
                     FSNGYMYALDAKTGRKIWSFRATDGYRDTKPSRPGDADHPPRAALDSYTESSQPKNRH
                     KFGSVHPGVDYPKPHGRSAVCTDENVAAFVTLSGWAYGLDATTGKPLWKRYVDAPDFP
                     GEMVWPEYKEGGALNPKNSSLGSSTRRFEAVPGLACGNGELLVLASDGHLRFLEPRTG
                     KLGPYNCGYSRPCESNGGGNVGPEYERLDRDSNDNPVDMCAASGFNCDMAIGLAIPPL
                     AASAAGNGTKGGDYLVSTLDSRLIRLSWDEHRPLWRREYPAPLPFQLGDTMPLVLNHL
                     EHGFITQAVIGGPMALDPDVAGKGARPILYAAAQDGQLYVLSVNELGGGLRGNDPGAP
                     KLLARIGISPNEQVETPYTRRGEGGPWDYNQHALAGLVLGGGVLYVPTWDNRMFAFDV
                     RDPSDPKKVWEHEIVWDKTFKYPPFGKTFDEPLADVDNKIWSSPALLGGRLYFTANDG
                     AVYAFNVRRPVRTVRNLVVLGSGLVPFLPG"
     gene            complement(1977..3215)
                     /locus_tag="VM841_04235"
     CDS             complement(1977..3215)
                     /locus_tag="VM841_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89426.1"
                     /translation="MRPRTLRPLAAALAAALTMSACTDAPKKVAQERVALRVGLLYTT
                     AGQGGDLASAVLGAAALATDAAAEDGVDIEIIEADYAGNPALAAEAAQGLATRSDAVV
                     VGTDDNAVAGSLGGATVPVMHPFITADAAVTGDGSAFRFAPPNRLQAARIVTFLVGHR
                     KYTRIGVLADDTPFGEEGVAGLREAFAGAGVTPVGTETFTPGGDVHTPLARMAQRNAQ
                     AVIVWVASPGEASRVVVDVHRSSYPYQLVLSGNMATPTFAKNASSQVAPVAFRDGILS
                     VGTWGGPWFPLERIVGFYREFRTRNSSIPPVQAASVYDAILALARAARNRGTSSENLV
                     RGLESLEDFEGAGTPVTFGPGKREGIDLDDVAMLGFTKNQAAPGGEFAPEVSTGGGFF
                     SIVHESLDLPAKYAYLANVS"
     gene            complement(3212..3910)
                     /locus_tag="VM841_04240"
     CDS             complement(3212..3910)
                     /locus_tag="VM841_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89427.1"
                     /translation="MARDLTGRVRLAIAVLATALTAAGCGVDDATLDGPRRAAAIHDG
                     VAMLTEPQFGGAGSTTTLGGEQLTDVEEAAPDGLRITTVRISPLSPVARQALKWSGFR
                     GGSSSALIELPISGINPQLGMYYLLVPVVNDGDEPVRNLKARADFFDADGVRVWTEVE
                     PVTYFPTRLGLNPPSLPEQRRIEEPSRLGSSIRGFSLYYFAGNVGLFTFAVPDPAIAA
                     TVRRWTLTFLVSTT"
     gene            complement(3895..6093)
                     /locus_tag="VM841_04245"
     CDS             complement(3895..6093)
                     /locus_tag="VM841_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89428.1"
                     /translation="MSLRDRAAGIAVVVAVVAALLPASVPAQTPSASPSPDSSPGATS
                     SAGPAASPAASESPSPTPEPVVEVQPGRSTDTEYLKVKIDRTGEPEAAWLKNWIRLRG
                     SGTRTLTDAGRFAGVTYLDGTPGARETPAGLRWRVGLGAAGFKDLYYEGRVTKVGDYW
                     MTASGLKPLPVEVRIRYYTGAEGSETEVAPEALGAGDGAQRFKIVVTLTNRTKRFEEV
                     SYNDIQTGRTMTATAPVSTPYVARIVDLRFPDDAYDRIQTDGEITRQSAATVVNWTRY
                     LIPPDFPEQQTAIVTGVISRGAPLPEIKVVAQPVYPAFDAEALNAEGTQFQRGRRNFF
                     YDVFGLFRENLVALTGLFGLLHDSFANLSLPLLGPEKGNREAGSFDEPNQLWALWTLT
                     KGIEQLDRAFNVFDSTSQLLRDAVKGSLATLAQLRAFVGYSTDKNIVTAFPPANANEA
                     LLGSIWSDLKTLASVCGEADWSLDTRPYFPETPLMTCPALPQVATSLNLIFLKLALVE
                     HDLQSVQKENHELDTALLAGLANTPFAGCGGPKDSDAGQTCDSYSKYLFIKFPFGLEE
                     IERALWLLKTEGFDAVQAAIGNKDQPNSLVQALHVLTEGTEAQIDAFHQLGATWRYVA
                     DSIQNFAIFGVESARNILQWDINAIDVDTAVKAASVRRAKQMSTFMGSFEDPDGEPAL
                     GQLVVTFSTAAGGERPVATDTRAGKTAVAFAGALLLATLALFGRFRWHVI"
     gene            complement(6324..7124)
                     /locus_tag="VM841_04250"
     CDS             complement(6324..7124)
                     /locus_tag="VM841_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL89429.1"
                     /translation="MRAAEIFERWYVATVLEPAVGRGRAIRRMAELAEIRDRLLREAT
                     IAPTADVLDLGCAYGLVTWPAGEEAGHAFGADPDPALIAAGMTVPATGATLLAADPDR
                     LPLPAASFDVVLWRGTLARRPAQAREILGEAFRVLRPEGRLCFAEPAPLEARPEDPAM
                     RDLWARLRAAPGGLFHRDGLETIVESAGFTGAHAKIERRRTTVEDERAAREVFEGAAP
                     GSVALGRLLAHAGMPETILRVYVETVASRAPVTLEVPEVYVTAEKPRR"
     gene            7373..8533
                     /locus_tag="VM841_04255"
     CDS             7373..8533
                     /locus_tag="VM841_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89430.1"
                     /translation="MRRLGQGFVVGAAFVLATGLWAPAGAGIVEHGIDSPADGEVTST
                     NVVPVQVRAESDSSGGLQYLRLELEGPGFSNRVVWCAPGPLPQCPAFSTNTKRVEGSQ
                     GRFNWDTTSLTTYNGDYTLKLVVKDNDGSIKTDVKQVRVNTPPAPPSWVSVSPTTTGG
                     QAKMTLKWRANAEPDITSYRITRTSSNGGPSTFFKVEKDNPGGQGCSLASGAFTCTDT
                     AFPASGYGGTYTYHLAATRHSPVTSSGVTSSQSTRAASVSEPKPSPSTGTGGGSGGGR
                     GGGGGSTPGSGGGGFITPGPGGAPGASMTPTPDPDAVDPREFYTGIFDKSLPYKQRIT
                     VVLGQQERRNVAGFASEDDIVPLDVRKTLLPIAGGLFTLTAALHFRRVIRDH"
     gene            8589..8789
                     /locus_tag="VM841_04260"
     CDS             8589..8789
                     /locus_tag="VM841_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89431.1"
                     /translation="MSGPVMCECGAVLVAEATPYGIVTVEGEYVKFSRKTDFIACAKC
                     LRSYPAREILPEPEFDEYLDGD"
     gene            complement(8786..9925)
                     /locus_tag="VM841_04265"
     CDS             complement(8786..9925)
                     /locus_tag="VM841_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89432.1"
                     /translation="MTRLRATLAVAALFATLLAPVQSRAGRTIDIEVFAAVISPRQLV
                     AQKGDLIRFTFTDGHFKLVTHASADFDDDPATDPTLINSPDVRVTRSQPPVVYVTEFQ
                     GGTIWYRNLHNSKLDENNNVCSGMCASITSIETMPSPPVITVPAAPVREKPASISGKA
                     DPLTLITLAEGTTVETGDYKGQALADEYGDWTVKTVNFGDTGGNKRLIARSVDARGYV
                     SADSQVAVVQYLPDFEPPEIVFDAPPLPVFFSEFEFTGTARDNVKVRAIAVQFIYLSP
                     NTGSPTNQNNTVLLPAVCNGCGTSNSVTWSFKKPVIGTFGAAGGLYQVTAFAWDTFDR
                     RASADAPSILGSGRLVLLASGTTVPDPTSTASPTPTPSISTPPLP"
     gene            complement(10169..12433)
                     /locus_tag="VM841_04270"
     CDS             complement(10169..12433)
                     /locus_tag="VM841_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL89433.1"
                     /translation="MDPLSFLERLRAADPESLAHVERIPERRAETAEVSLTPEISVRL
                     KKAGIGALWAHQAEALEHLRAGRHTAVATGTASGKSLCYQLATLERLQAGPKATALYL
                     FPTKALAQDQLRQLRSFAATWVRAAVYDGDTAPADRQWIKRNANLIVSNPDMLHFGVL
                     PQADKWAPFFANLALIVVDEMHTFRGVFGSHVANVLRRVRRLAEARGAAPLFACASAT
                     IGNPGELASRLTGLDFEVVTRDGSPRGTRFFALWNPPVDPDRVPPSRTGAGAEAAELM
                     ARTIGEGANTIAFAKSRRGAEMVAQVARSRLERDDRHDLAGRVAAYRAGYLAEERRAL
                     ERALADGDLAGIAATTALELGIDIGGLDACIIAGYPGTIAATMQQAGRAGRRQGRSLA
                     MLVANDDPLDQYLMNHPDALFGRPIEAAILDHSNPNIMDPHVACAAYEQPIDEQADLA
                     YFGPALAGSIARLREAGLLRPRAGKWFYSGRTGPHPDMDIRSMGGEVRIVEAATGALI
                     GTASGARAPWSVHPGAIYLHQGEQYRVATLDLDAGVALVEPNDEPIYTQPRDVTDIRV
                     LSVDRKAPLGATEVFFGTVEVTNQVVAYARKHVFSGEIIDVTPLELPPRTLTTHAVWY
                     TAPHELLDRARIDPMDVPGAAHAAEHAAIGILPLFAMCDRWDIGGVSTAMHGDTGHCT
                     VFIYDGYPGGAGIAARAFGAAEAHQRATMDAIRQCPCETGCPSCVQSPKCGNGNSPLD
                     KLAAVRLLEAVLGD"
     gene            complement(12492..13370)
                     /locus_tag="VM841_04275"
     CDS             complement(12492..13370)
                     /locus_tag="VM841_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL89434.1"
                     /translation="MHSQLKFLAVLTAAFAIALAAALPAAGESDAGRLTQLHDTERSK
                     HSLSSLARAGDLAGDAQVHAREMAGRGEIYHSGRTSRLEGWYALGENVGWGSDVDEVF
                     RMFMESPSHRANILDGSWDSIGVGVAAGESGEVYVAVLFGDREDAAARPAPQAEQPKK
                     PAVKKQPVKAVKAPERKRVPAAPTPAPSVRTTPIPTPEASAAPEVPAPPAPPVPAEPR
                     PSDASRAVGVLLAVEREHREPLPEEAATIGDVSASTARRLFSESEAERRRQAAARPEG
                     AAARSLGGVARCAWSC"
     gene            complement(13517..14635)
                     /locus_tag="VM841_04280"
     CDS             complement(13517..14635)
                     /locus_tag="VM841_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HVL89435.1"
                     /translation="MIRRLSPAARLGALALATLLASLAMWSVVSLSTAEGQEKAVRIR
                     RTHNGYFRPEPNRPIFVLAIGSDAGSPRYKRDGPVDRGRADSIHVIAINPQTKRGTIV
                     GIPRDAYVNVRGYAGRINGALTAGGMDRMVDVVEQFTGLTMDYSMLVSFEGFEFMINE
                     LGGVVVDVPYDIRDPKSRAKFDAGQQVLGGFQALAFARNRYSTPRGDFSRSENQGLIM
                     LGALRRARAETFEDPGKFLHYLRILYKYVDTDLPVDEALQLGLMVTRIQTTDIENVVV
                     DGYPTMTSDGASVVQLTKNARRLFDDIAPDAVIDDRSVLDPGPRPSMPRAVPEETDEP
                     LPEPDESTEPSPDPSESAEATPDPQPSETASPTPRASL"
     gene            complement(14632..15243)
                     /locus_tag="VM841_04285"
     CDS             complement(14632..15243)
                     /locus_tag="VM841_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89436.1"
                     /translation="MLPLSRPISSRVLAVCLILAAFGSAACSGSPAGSDEVVQLAFQD
                     SVEVVAAGDAEPPAEARDQGGAIVGLITEWYQRAFVDPAGWRDEKFPDVVRLFSGEAR
                     TKVKREIESVTIGDARDEVRSVKPEDATVKVTVYVGEDGRTDYAVADVSFHGTGALKK
                     EGVPLTIVQKATYFFRLESDGWRIFAYQARSDHAQNPPPGATS"
     gene            15438..18020
                     /locus_tag="VM841_04290"
     CDS             15438..18020
                     /locus_tag="VM841_04290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HVL89437.1"
                     /translation="MSTILAAEGGSINVIFRELENNMLWVILGISLLALAVAYYLVKG
                     VLREPEGTPKMIEIAKAIQEGSAAYLNRQFKTVGLFVGALAVLLFFILPVPQQCPGGV
                     QISLSETCADNSEMVLKLGRSIAFVLGAAFSALTGFAGMSLAVRGNVRVANAARESGL
                     RQALTIAFRTGGVAGMFTVGLGLLGATVVLIIFKRDATQVLVGFGFGGALLAMFMRVG
                     GGIFTKAADVGADLVGKVEKGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYEVT
                     LVASIILGAAAFADRGIEFAIVGVMFPLIVRAIGVITSVLGIYMVKPRSENEHGMKSI
                     NRGFFVSAIVSAVAIYFAAAWYVKDIRVFWATVIGLGLSAAIQVLTEYYTSTTRKPVI
                     EIAEASETGPATTILSGFSVGLESTVWALMVIAAAIFGAFLLGDNLAERLYFISLSGM
                     GMLTTVGVIVSMDTYGPVSDNAHGIAEMSGGLGERADEIMAGLDAVGNTTKAITKGMA
                     IATAVIAATSLFGSFFEVLKEAGSPVTAIRVDNPDVLVGLLIGGSIAFLFSSLAIRAV
                     SRAAGQVVVEVRNQFRENPGIMDGTVKPDYGRVVDICTKTSLRELMTPGLLAVASPLI
                     VGFAFKEEALGAFLAGVIVTGQLLAVMLSNAGGAWDNAKKLVEEGHHGGKGSEAHKAT
                     VIGDTVGDPFKDTAGPALNPLIKVMNLVALLIAPIVVEHAGDPLWRYGVPLVAGTMLG
                     LSIWQSKRRKIDFMVSAPAGVGSAAPVTATAAVVESAMATLPPPVETAAEHREELAEI
                     EGEDDDLDEPVPARRAPAKTATRRPAAKKTAAKKTAAKKTTARKAPAKRSASKTTSSR
                     TRKD"
     gene            18100..18498
                     /locus_tag="VM841_04295"
     CDS             18100..18498
                     /locus_tag="VM841_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVL89438.1"
                     /translation="MGIFERFFRRLAVDAESIRVAEVGRWAQEIPGVVSIGEAEPRAE
                     VKVAGVVERLRVRPQKGVPAIEAVLVDGTGMCTAVWLGRRSIKGLELGRRMVIEGRLA
                     VKDGDRQIMNPTFEFADQRVIEERMKRKKD"
     gene            complement(18495..>19039)
                     /locus_tag="VM841_04300"
     CDS             complement(18495..>19039)
                     /locus_tag="VM841_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HVL89439.1"
                     /translation="TGSIRAAAAIYAIALSGLFGVSASYHRLPWSDKARVRMQALDHS
                     AIYLLIAGTYTPLSLLVLEGAWRWTMLGIAWSGALAGTAIKTSRVRWGDGLGFALYLI
                     LGWTVVLAMPQIVRNMHAALLALLVAGGVLYTVGAILFALRKPVLRPATFGFHEIWHV
                     FVLSAATCHYVVVTGLVATR"
BASE COUNT         2847 a         6503 c         6778 g         2911 t
ORIGIN      
        1 acccgggaag gaacggcacc agccccgagc cgagcacgac caggttgcgc acggtccgga
       61 ccggccggcg cacgttgaag gcgtagacgg cgccgtcgtt cgcggtgaag tacagccggc
      121 cgccgagcag cgccggcgag gaccagatct tgttgtccac gtcggcgagc ggctcgtcga
      181 aggtcttgcc gaacggcggg tacttgaagg tcttgtccca gacgatctcg tgctcccaga
      241 ctttcttcgg atccgacggg tcgcgcacat cgaaagcgaa catcctgttg tcccacgtgg
      301 gcacgtacag cacgccgccg ccgagcacca gcccggcgag cgcatgctgg ttgtagtccc
      361 acgggcctcc ctcgccgcgc cgcgtgtagg gcgtctcgac ctgttcgttg ggtgagatgc
      421 cgatgcgcgc gagcagcttc ggcgctcccg ggtcgttgcc gcgcaagccg ccgccgagct
      481 cgttgaccga tagcacgtac agctggccgt cctgcgcggc cgcgtacagg atcggccgcg
      541 cgcctttgcc ggcgacgtcc ggatcgagcg ccatcggtcc gccgatcacc gcctgcgtga
      601 tgaagccgtg ctcgagatgg ttcaggacga gcggcatcgt gtccccgagc tggaacggaa
      661 gcggcgccgg atactcgcgg cgccacagcg gccgatgctc gtcccacgac agccggatga
      721 ggcgagagtc gagcgtgctc acgagatagt cgccgccctt ggtgccgttg ccggcggcgg
      781 aggcggccag cggcgggatc gccagcccga tcgccatgtc gcagttgaag ccgctcgccg
      841 cgcacatgtc caccggattg tcgttcgaat cgcgatcgag ccgctcgtac tcgggaccga
      901 cgttgccgcc gccgttcgac tcgcacgggc gggagtagcc gcagttatac ggaccgagct
      961 tgccggtgcg cggctcgagg aaccgcaagt gcccgtccga ggcaaggacc aggagctcgc
     1021 cgttgccgca cgcgagcccc ggcaccgcct cgaagcggcg cgtggaggac ccgagcgacg
     1081 aattcttcgg gttgagcgcc ccgccctcct tgtactcggg ccagaccatc tcgcccggga
     1141 agtcgggcgc gtccacgtaa cgcttccaca gcggcttgcc cgtcgtggca tccagcccgt
     1201 aggcccagcc cgacagcgtc acgaacgcag cgacgttctc atccgtgcag acggcgctgc
     1261 gtccgtgcgg cttggggtag tccacgccgg gatgcaccga tccgaacttg tgccggttct
     1321 tcggctgcga cgattccgtg taggagtcga gcgccgctcg cggtggatgg tcggcatcgc
     1381 cgggacgcga cggcttggtg tcacggtagc cgtcggtcgc gcggaacgac cagatcttgc
     1441 gtccggtctt ggcgtcgagc gcgtacatgt agccgttgct gaacgtggtg tagatcaggc
     1501 cattgtggat cgagggggcg ttcgagctcg gatattgcgc ggtgacgccg tcggtgcgcg
     1561 ggtccggcga gaagcgccac actggaaggc ccgtgtccat gtcgagagcc aggaacgagt
     1621 tggtcgcgcc gccgccgacg tagagcaggc cgtcgtgcgc gacgatcgcc gcggtcggga
     1681 acgcgccggt gggcaggtcg ctgatcttgg tgacccactc cagccacggg ccgtcggcgt
     1741 cgccggtctt gcggcacgcg gtgccgcgac agccgatgaa cggcggcggc gtctcctgcg
     1801 gcgcgtagcc ggtcaggaag ttgtcgccgc cgggcgacat cggccagctc gtgtcggtgt
     1861 tcgggatcca ggtcgagggc cggcgcgtcg cggccggcgc gggcgggacg gccacgccga
     1921 gcacggcgat cacggccgcg gcgacgatcc atcgcggctt cacggtcggt gccctcctag
     1981 gagacattgg ccagataggc gtacttcgcg ggcaggtcca gggactcgtg cacgatgctg
     2041 aagaatccgc cgccggtgct gacctcgggt gcgaactcgc cgccgggcgc ggcctggttc
     2101 ttggtgaagc cgagcatcgc gacgtcgtcg aggtcgatgc cctcgcgctt gcccggcccg
     2161 aaggtgacgg gcgtgcccgc tccttcgaag tcctcgagcg actcaaggcc tcgcaccagg
     2221 ttctcggaac tcgtgccgcg gttgcgagcg gcgcgggcga gcgccaggat cgcgtcgtag
     2281 accgacgcgg cctgcacggg cgggatcgag gagttgcggg tgcggaactc ccgatagaag
     2341 ccgacgatgc gctcgagcgg gaaccacggg ccgccccacg tgccgaccga caggatgccg
     2401 tcgcggaagg cgaccggcgc gacctgagag ctcgcgttct tcgcgaaggt cggcgtcgcc
     2461 atgttgcccg acagcacgag ctgatacggg taggaggagc ggtgcacgtc cacgacgacc
     2521 cgtgaggctt cgccgggcga ggcgacccag acgatgaccg cctgggcgtt gcgttgcgcc
     2581 atccgcgcga gcggtgtgtg gacgtcgccg cccggcgtga acgtctcggt cccgaccggc
     2641 gtgacgcccg cgccggcgaa tgcctcgcgc agcccggcca ccccttcctc gccgaacggt
     2701 gtgtcgtcgg cgagcacgcc gatgcgcgtg tacttgcgat ggccgacgag gaacgtcacg
     2761 atgcgcgcgg cctgcagcct gttcggcggc gcgaagcgga acgcggaccc gtcgccggtc
     2821 acggcggcgt cggcggtgat gaacggatgc atcaccggga ccgttgcccc gccgagcgag
     2881 ccggcgacgg cgttgtcgtc cgttccgacc acgaccgcgt ccgagcgggt cgccagtccc
     2941 tgcgcggcct cggcggcgag cgccgggttg ccggcgtagt cggcctcgat gatctcgatg
     3001 tcgacgccgt cctctgcggc cgcgtccgtc gcgagcgccg ctgcgccgag cacggcgctc
     3061 gcgaggtcgc cgccttgccc ggccgtggtg tagagcagcc cgacgcgcag cgccacacgc
     3121 tcctgtgcga ccttcttcgg cgcgtccgtg catgccgaca tcgtcagcgc ggccgcgagc
     3181 gcggcggcga gcgggcggag cgtgcgcggc ctcatgtcgt gctcaccagg aaggtcagcg
     3241 tccagcgacg aaccgtggcg gcgatggccg gatcgggaac cgcgaacgtg aacagtccga
     3301 cgttgccggc gaagtagtac agcgagaagc cgcggatcga ggagccgagc cgggacggct
     3361 cctcgatccg ccgctgctcg ggaaggctcg gtgggttcag ccccagtcgc gtcgggaagt
     3421 acgtgaccgg ctcgacctcg gtccacacgc gcacaccgtc ggcgtcgaag aagtcggcac
     3481 gggccttgag gttgcgcacc ggctcgtcgc cgtcgttgac caccggcacg agcaggtagt
     3541 acatgcccag ctgcgggttg atgccgctga tcggaagctc gatcagcgcc gaggacgatc
     3601 cgccgcgaaa gcccgaccac ttcagcgcct gccgcgcgac cggcgacagc ggcgagatcc
     3661 ggaccgtggt gatgcgcagc ccatccggcg cggcctcctc gacgtcggtg agctgctcgc
     3721 cgcccagcgt cgtcgtcgag cccgcgccgc cgaactgcgg ctcggtgagc atcgccacac
     3781 cgtcgtggat tgcggccgct cgccgcgggc cgtccaatgt cgcgtcgtcg acgccgcagc
     3841 ccgccgccgt cagcgcggtc gcgagcaccg cgatcgcgag gcggacgcgc ccggtcagat
     3901 cacgtgccaa cggaacctcc cgaacagcgc gagcgtggcc agcagcaagg caccggcaaa
     3961 cgcgaccgcg gtcttgccgg cgcgcgtatc ggtcgcgacc gggcgctcgc cgccggccgc
     4021 ggtcgaaaac gtcaccacga gctggccgag cgccggctcg ccatcgggat cctcgaacga
     4081 tcccatgaac gtggacatct gcttcgcgcg ccgcaccgat gccgccttga ccgcggtgtc
     4141 cacgtcgatg gcgttgatgt cccactgaag gatgttgcgg gcgctttcga cgccgaagat
     4201 agcgaagttc tggatcgagt ccgcgacgta ccgccaggtc gcgccgagtt gatggaacgc
     4261 gtcgatctgc gcctcggtgc cttcggtgag cacgtgcagc gcctgcacga gcgagttggg
     4321 ttgatccttg ttgccgatcg cggcctgcac cgcgtcgaag ccctcggtct tgagcagcca
     4381 gagcgcgcgc tcgatctcct ccaggccgaa cggaaacttg atgaacaggt acttcgagta
     4441 cgagtcgcag gtctgcccgg cgtcgctgtc cttgggcccg ccgcatcccg cgaacggcgt
     4501 gttcgccagg ccggcgagca gcgcggtgtc gagctcgtgg ttctccttct gcaccgactg
     4561 cagatcgtgc tcgaccagcg cgagcttgag gaagatcagg ttcagcgacg tcgcgacctg
     4621 cggcagcgcg gggcacgtca tgagcggcgt ctccgggaag tagggccgcg tgtccagcga
     4681 ccagtccgcc tcgccgcaga cgctcgcgag cgtcttgagg tcgctccaga tcgagccgag
     4741 gagcgcttcg ttcgcgttcg ccggcgggaa cgccgtcacg atgttcttgt cggtcgagta
     4801 ccccacgaac gcgcgcaact gcgccagcgt cgccagcgat cctttcaccg catcgcgaag
     4861 aagctgcgag gtcgagtcga acacgttgaa cgcgcggtcg agctgctcga tgcccttcgt
     4921 gagcgtccac aaggcccaca gctggttggg ctcgtcgaac gatccggctt cccggttgcc
     4981 cttttcgggc cccagcagcg gcagcgacag gttcgcgaac gagtcgtgga gcagcccgaa
     5041 caggccggtg agcgcgacca agttctcgcg gaacaggccg aacacgtcgt agaagaagtt
     5101 gcggcggccg cgctggaact gcgttccctc tgcgttgagc gcctcggcgt cgaacgccgg
     5161 atagacaggc tgcgcgacca ccttgatctc gggcagcggc gcgccgcgcg agatcacgcc
     5221 cgtcacgatc gcggtctgct gctccgggaa gtcgggtggg atcaggtagc gcgtccagtt
     5281 caccacggtc gcagcgctct gccgcgtgat ctcgccgtcg gtttgaatgc gatcgtaggc
     5341 atcgtcgggg aagcggaggt cgacgatgcg cgcgacgtag ggcgtggaga ccggcgcggt
     5401 cgcggtcatg gtgcggccgg tctgaatgtc gttgtacgag acctcttcga agcgcttcgt
     5461 gcggttggtg agcgtgacga cgatcttgaa acgctgcgct ccgtccccgg cgccgagcgc
     5521 ctcgggcgcg acctccgtct cggatccttc ggcccccgtg tagtagcgga tgcggacctc
     5581 gaccggaagc ggcttgaggc cgctcgcggt catccagtag tcgccgacct tggtgacgcg
     5641 gccttcgtaa tagagatcct tgaagccggc ggcgccgagc ccgacccgcc agcgcagccc
     5701 cgccggcgtc tcgcgggcgc ccggcgttcc gtccagatag gtcacgccgg cgaagcggcc
     5761 ggcgtcggtc agggttcggg tgccgctgcc gcgcagccgg atccagttct tcagccacgc
     5821 ggcttcgggc tcgccggtac ggtcgatctt gaccttcagg tattcggtgt cggtcgagcg
     5881 gcccggctgc acctccacga ccggctcggg ggtcggagac ggcgactcgc tcgcggccgg
     5941 agaggccgcc gggccggcgg aggaggtcgc gccgggcgac gagtccgggg acggggaggc
     6001 ggacggtgtc tgggccggga cggacgccgg gagcagcgcg gcgacgaccg cgacgaccac
     6061 ggcaatgccg gccgcgcgat cccgcaagct caagcgacgc cctccccgac ggatgcgagc
     6121 atccgtccgt agccccgtga cgagtgacaa cgacgtacgg cggacacgcg gccgcgccgc
     6181 acgctccaat cagccgggcc ccgattccga agccttttct aaacgttcgc agggggagag
     6241 gcaaggacgc cgccctcgct gcagcgggtt gaccctcgtc gccaggcgag ctgcacgagc
     6301 gcacgttctg tggacggctg ggatcagcgg cggggcttct cggcggtcac gtacacctcg
     6361 ggaacctcga gcgtgaccgg ggcgcgggac gcgaccgtct cgacgtagac gcgaaggatc
     6421 gtctcgggca tgccggcgtg agccagtagc cgtcccagcg cgaccgaacc gggtgctgcc
     6481 ccttcgaaca cctcgcgcgc ggcgcgctcg tcctcgacgg tggtgcggcg gcgctcgatc
     6541 ttggcgtgcg cgccagtgaa cccggccgac tcgacgatcg tctcgaggcc gtcccggtgg
     6601 aacagcccgc cgggcgcggc gcgcagccgg gcccacagat cccgcatcgc gggatcctcc
     6661 ggccgtgcct cgagcggggc gggctcggcg aagcagagcc ggccctcggg gcgcaggacc
     6721 cggaacgcct cgccgaggat ctcgcgggcc tgagccggcc ggcgcgcgag cgtgccccgc
     6781 cacaggacca cgtcgaacga agcggccggc agcggcagcc gatccgggtc cgccgcgagc
     6841 agcgtcgccc cggtcgccgg cacggtcatg cccgccgcga tcagcgccgg atcggggtcg
     6901 gcgccgaagg catggccggc ctcctcgccg gcgggccagg tgaccaagcc gtacgcgcag
     6961 ccgaggtcga gcacatcggc ggtcggcgcg atggttgcct ccctgagcag ccggtcgcgg
     7021 atctcggcca gctcggccat ccgccggatc gcgcggcctc ggccgacggc gggctcgagc
     7081 accgtcgcca cgtaccatcg ctcgaagatc tccgcggctc gcatcgcccg gatgatagag
     7141 cccgcagacg tgaccccggc cggggataaa ccacgcatag ccgacccaag acggatcctg
     7201 cgtgactccg ggcgcgtcga agcgttgtct aggcagggcg ggctatgggc ctcaccgtcc
     7261 tctcaggctg cagcgccaca aaggacacgt acgaatcggg agacaccggg ctgaaggcag
     7321 ttctcccgta gctatactgg acaattgact gaatgcgcag gtcagggcac agatgagacg
     7381 actgggacag ggattcgtag tgggggcggc cttcgtcctc gcgaccgggt tgtgggcgcc
     7441 tgcgggcgcc gggatcgtcg agcacggcat cgactcgccg gccgacggcg aggtgacgtc
     7501 gacgaacgtc gtcccggtgc aggtccgcgc ggaaagcgac tcgagcggcg ggctgcaata
     7561 tctgcgtctc gagctcgaag gtcccggctt ttcgaaccgc gtcgtctggt gcgcgcccgg
     7621 accgttgccg cagtgcccgg ccttctcgac caacaccaaa cgcgtcgagg gttcccaggg
     7681 ccgcttcaac tgggacacga cctcgctgac cacctacaac ggtgattaca cgctcaagct
     7741 cgtggtcaag gacaacgatg gctcgatcaa gaccgacgtc aagcaggtcc gcgtcaacac
     7801 gccgcccgcg ccgccgtcgt gggtttcggt ttccccgacc accacgggcg gacaggcgaa
     7861 gatgacgctg aagtggcggg ccaacgccga gccggacatc acctcctacc ggattacgcg
     7921 cacgtcctcg aacggcggcc cgagcacgtt cttcaaagtg gagaaggaca accccggcgg
     7981 gcaaggatgc tcgctggcga gcggcgcctt tacgtgcacc gacaccgcct tcccagcgag
     8041 cggctacggc gggacctaca cctaccatct cgccgcgacg cggcacagcc cggtgacgag
     8101 ctccggggtg acctcgagcc agtccacgcg tgcggccagc gtctccgagc cgaagccttc
     8161 gccgtccacc ggaaccggcg gcggcagtgg cggcggccgt ggcggcggcg gcggatccac
     8221 ccccggctcc ggcggcggcg gcttcatcac gcccggcccc ggtggagcgc ccggcgcgtc
     8281 gatgacgccc acgcccgatc cggacgcggt cgacccgcgc gagttctaca ccggcatctt
     8341 cgacaagagc ctgccctata agcagcgcat cacggtcgtg ctcgggcagc aggagcgccg
     8401 caacgtcgcc ggtttcgcga gcgaggacga catcgtgccg ctcgacgtgc gcaagacgct
     8461 cctgcccatc gcggggggtc tgttcacgct caccgccgcc ttgcacttcc gccgcgtcat
     8521 tcgcgaccac tagcggaacg gcccgccgcg cggcccgagg gcagccggtg cgttaccctg
     8581 ccatcaggat gagcggacca gtcatgtgcg agtgcggcgc cgtgctcgtc gccgaggcca
     8641 cgccctacgg gatcgtcacc gtggaaggcg agtacgtgaa gttctcgcgc aagacggact
     8701 tcatcgcctg cgccaagtgc ctgcgctcgt atcccgcccg cgagatcctg cccgaacccg
     8761 agttcgacga gtacctcgac ggcgactagg gcagcggcgg cgtcgagatc gacggcgtgg
     8821 gcgtgggtga cgccgtggac gtcggatccg gaacggtggt tccggaggcg agcaggacca
     8881 atcgtcccga acccaggatg ctcggtgcgt ccgcgctcgc tcttcggtcg aaggtgtccc
     8941 aggcgaacgc cgtcacctga tacagcccgc cggccgcccc gaacgtgccg atcaccggct
     9001 tcttgaagct ccaggtgacc gagttcgagg tgccgcatcc gttgcagacg gccggcagca
     9061 gcacggtgtt gttctggttc gtcgggctgc ccgtgttggg gctgaggtag atgaactgca
     9121 ccgcgatcgc gcgcaccttg acgttgtcgc gcgcggtgcc ggtgaactcg aactcggaaa
     9181 agaagaccgg cagcgggggc gcgtcgaaga cgatttcggg cggctcgaag tcgggcagat
     9241 actggaccac cgccacctgc gagtccgcgc tcacgtagcc gcgcgcatcc accgatcgtg
     9301 cgatcagccg tttgttgccg ccggtatcac cgaagttcac ggtcttgacc gtccaatccc
     9361 cgtactcgtc cgcgagcgcc tggcctttgt agtcgccggt ctccacggtg gtgccttcgg
     9421 cgagcgtgat caacgtgagc gggtcggcct tgcccgagat cgacgcgggc ttctcccgca
     9481 cgggcgcggc gggcaccgtg atcaccggcg gagagggcat cgtctcgatg gacgtgatcg
     9541 acgcgcacat gcccgagcaa acgttgttgt tttcgtcgag cttggagttg tgcaggttgc
     9601 gataccagat cgtgcctccc tggaactccg tcacgtagac gaccgggggc tggcttcggg
     9661 tgacgcgcac gtcgggcgag ttgatcaggg tcgggtcggt ggcggggtcg tcgtcgaagt
     9721 cggcggaggc gtgcgtgacc agcttgaagt gcccgtcggt gaaggtgaac cggatcaggt
     9781 cgcccttctg cgccaccaac tggcgtggcg agatgacggc ggcgaacacc tcgatgtcga
     9841 tcgtgcgtcc ggctcgtgac tggacgggcg cgagcagggt cgcgaacagg gcagcgacgg
     9901 caagggtcgc gcgaagacgg gtcataccgg ttctagtacg acacgccggg cgaaagtcct
     9961 gacgacggtc gttccgcctt ggccgccttg gcccgattgc cctgcgaata gtgcggattc
    10021 ttcgttattc gagggcgagc cgcaccgcct ccgccgcgtc cgggacgcgg cggaggccct
    10081 cggccgccgc gccgggccgc gcgagctcaa aggtcgatcc cgatgaccgg aatccgcgtc
    10141 ttcagcgcca cgccgatgga gcgtctcatc agtcgccgag caccgcctcc agcagccgca
    10201 cggcggcgag cttgtcgagc gggctgttgc cgttgccgca cttgggcgac tgcacgcacg
    10261 acgggcagcc ggtctcgcag gggcactgcc ggatcgcgtc catcgttgcg cgctggtgcg
    10321 cctcggcggc gccgaacgcg cgcgccgcga tgccggcgcc gccggggtag ccgtcgtaga
    10381 tgaacacggt gcagtgcccg gtgtcgccgt gcatcgcggt cgagacgccg ccgatgtccc
    10441 accggtcgca catcgcgaac agcggcagga tgccgatcgc ggcgtgctcg gccgcgtgcg
    10501 cggcacccgg cacgtccatc ggatcgatcc gggcgcggtc gagcagctcg tggggcgcgg
    10561 tgtaccagac tgcgtgtgtc gtcagtgtcc gcggcggcag ctccagcggg gtcacgtcga
    10621 tgatctcgcc cgagaacacg tgcttgcgcg cgtaggcgac gacctgattg gtgacctcga
    10681 cggttccgaa aaacacctcg gtggccccga gcggcgcctt gcgatcgacc gagagcactc
    10741 ggatgtcggt cacgtcgcgc ggctgcgtat agatgggctc gtcgttcggc tcgaccagcg
    10801 ccacgccggc gtccaggtcg agcgtggcca cgcggtattg ctcgccctga tgcagataga
    10861 tcgcacccgg atgcaccgac cacggcgcgc gcgcgccgct cgccgtgccg atcagcgcgc
    10921 cggtcgcggc ctcgacgatg cgcacctcgc cgcccattga gcgaatgtcc atgtccgggt
    10981 gcggaccggt gcggcccgag tagaaccact tgccggcgcg cggccgcagc aggcccgctt
    11041 cgcgcagccg cgcgatcgag cccgcgagcg ccgggccgaa gtacgcgagg tcggcctgct
    11101 cgtcgattgg ctgctcgtag gccgcgcacg ccacgtgcgg atccatgatg ttcgggttgg
    11161 aatgatccaa gatcgcggcc tcgatgggcc ggccgaacag cgcgtccgga tggttcatca
    11221 ggtactggtc gagcggatcg tcgttggcga cgagcatcgc gagcgaccgc ccctgccggc
    11281 ggcccgcccg cccggcctgc tgcatcgtcg cggcgatcgt gcccgggtag ccggcgatga
    11341 tgcacgcgtc gagcccgccg atgtcgatgc cgagctcgag cgcggttgtg gccgcgatgc
    11401 cggccaggtc gccgtcggcg agcgcccgct cgagcgcgcg gcgctcctcg gccaggtagc
    11461 cggcgcggta cgcggcgaca cggccggcga ggtcgtgccg gtcgtcgcgc tccagccgcg
    11521 agcgcgcgac ctgcgcgacc atctcggcgc cgcgccgcga cttcgcgaac gcgatcgtgt
    11581 tcgcgccctc gccgatcgtg cgggccatca gctcggccgc ctccgcgccc gcaccggtgc
    11641 gcgacggcgg cacccggtcg ggatcgaccg gcgggttcca cagcgcaaaa aaacgcgtgc
    11701 cgcgcggcga gccatcccgc gtgacgacct cgaagtccag gccggtcagg cgcgaggcga
    11761 gctcgcccgg gttgccgatc gtcgccgatg cgcacgcgaa cagcggcgct gcgccgcgcg
    11821 cctcggcgag ccggcgcacc cggcgcagca cgttcgccac gtgcgagccg aacacgccgc
    11881 ggaacgtgtg catctcgtcc acgacgatca gcgcgaggtt ggcaaaaaac ggcgcccact
    11941 tgtcggcctg cgggagcacg ccgaaatgga gcatgtccgg gttggacacg atcaggttcg
    12001 cgttgcgctt gatccattgc cggtcggcgg gcgcggtgtc gccgtcgtat accgccgcgc
    12061 gcacccacgt cgccgcgaag ctgcgcaact gccgcagctg gtcctgggcg agcgccttgg
    12121 ttgggaaaag gtagagggcg gtggctttcg ggccggcctg gagtcgctcg agcgtggcga
    12181 gttgatagca caggctcttg ccgctcgcgg tcccggtcgc gaccgcggtg tggcggccgg
    12241 cgcgaagatg ctcgagcgcc tcggcctgat gcgcccagag cgcgccgatc cccgccttct
    12301 tcagccggac cgagatctcg ggcgtgagcg agacctcggc ggtttcggcg cggcgctccg
    12361 ggatgcgctc gacgtgggcg aggctttcgg gatccgcggc gcgcagtcgt tccaggaagc
    12421 tgaggggatc cacccgccaa tcgtatcgcc gcccggcgac ggccgggacg ccgagatcag
    12481 ccggaagcgg gctagcagga ccaggcgcag cgcgccacgc cgcccaggct gcgcgcggcg
    12541 gcgccttcgg gtcgcgcggc ggcttgccgg cgacgctcgg cctcggattc cgagaacagg
    12601 cggcgcgcgg tgctcgccga cacgtccccg atcgtggcgg cctcctcggg cagcggctcg
    12661 cggtgctcgc gctcgacggc gagcaggacg ccgaccgcgc ggctcgcgtc gctcgggcgg
    12721 ggctcggccg gcaccggagg cgccggcggg gcgggtacct ccggcgcggc gctcgcttcg
    12781 ggcgtcggga tcggcgtggt gcgaaccgat ggcgccggcg tcggggctgc cgggacgcgc
    12841 ttgcgctcgg gcgccttgac ggctttgacg ggctgcttct tcaccgcggg cttcttgggc
    12901 tgctcggcct gcggggccgg gcgtgcggcc gcgtcctcgc ggtcgccgaa caagaccgcg
    12961 acgtagacct cgccgctttc gccggccgcc acgcccacgc cgatcgagtc ccacgagccg
    13021 tcgaggatgt tggcgcgatg cgacggcgac tccatgaaca tgcggaagac ctcgtcgacg
    13081 tcgcttcccc agccgacgtt ctcgccgagc gcgtaccagc cttccagccg cgaggtccgg
    13141 cctgagtggt agatctcgcc gcggccggcc atctcgcggg cgtggacctg tgcgtcgccg
    13201 gccaggtcgc ccgcgcgcgc gagcgacgat agcgagtgct tgcttcgctc ggtgtcgtgc
    13261 agctgcgtga gacggcccgc atcggactcg cccgcggcag gaagcgcggc ggcgagcgcg
    13321 atggcgaagg ccgcggtcag cacggccaaa aacttcagct gtgaatgcat gcggtctccc
    13381 ctcctgcatg cctcgacgcg gacgagcccg gaatcttgca cgctccttcg cgaaatcgcc
    13441 agaaagggtc ctgcctctgc ttgccccgtt gcccggggtg ccaatcgcga cccggcgagc
    13501 gcttcctttc gcccggctag agcgaggctc gcggcgtcgg cgaggccgtc tcggacggct
    13561 gcgggtcggg tgtggcttcg gccgactccg acgggtccgg gctcggttcg gtggactcgt
    13621 ccggctcggg cagcggctcg tcggtctcct ccggcacggc gcgcggcatc gacgggcgcg
    13681 ggcccgggtc caggacgctc cgatcgtcga tgaccgcgtc gggcgcgatg tcatcgaaga
    13741 gtcgccgggc gttcttggtg agctgcacca cgcttgcgcc gtcggaggtc atcgtcgggt
    13801 agccgtcgac gacgacgttt tcgatgtcgg tcgtttggat gcgcgtgacc atcaggccga
    13861 gctgcagggc ctcgtcgacc ggcaggtcgg tgtccacgta cttgtagagg atccgaaggt
    13921 agtgcaggaa cttgccggga tcctcgaagg tctcggcccg cgcgcgccgc agcgcgccga
    13981 gcatgatgag cccttggttc tccgaccgcg agaagtctcc gcgcggcgtg ctgtagcggt
    14041 tgcgcgcgaa cgccagcgcc tggaagccgc cgagcacctg ctggccggcg tcgaatttcg
    14101 cgcgggactt cgggtcgcgg atgtcgtagg gcacgtccac gaccaccccg cccagctcgt
    14161 tgatcatgaa ctcgaagccc tcgaaggaca ccagcatcga gtagtccatg gtcaggccgg
    14221 tgaactgctc gacgacgtcg accatgcggt ccatgccgcc ggccgtcagc gccccgttga
    14281 tccggcccgc gtagccgcgc acgttcacgt acgcgtcacg ggggatcccc acgatcgtgc
    14341 cgcgcttggt ttgcgggttg atcgcgatga cgtggatcga gtcggcgcgg ccgcggtcta
    14401 cggggccatc gcgcttgtag cgcggcgagc cggcgtcgga gccgatggcc agcacgaaga
    14461 tcggccggtt cggctcggga cggaagtagc cgttgtgagt gcggcggatc cgcacggcct
    14521 tctcctggcc ctcggccgtg gacagcgaca ccacgctcca catcgccagc gaggcgagca
    14581 gcgtcgccag cgcgagcgcc ccgaggcgcg ccgcggggga aaggcgccgg atcacgacgt
    14641 agctcccggc ggcgggttct gcgcgtggtc gctgcgggcc tggtaggcga agatccgcca
    14701 gccgtcgctt tcgagccgga agaagtaggt ggctttctgc acgatcgtca ggggcacgcc
    14761 ctccttcttg agcgcgccgg ttccgtggaa ggagacgtcg gcgacggcgt agtcggtgcg
    14821 gccgtcctcg cccacgtaga ccgtgacctt gaccgtggcg tcctcgggct tgaccgagcg
    14881 gacctcgtcg cgggcgtcgc cgatggtgac cgactcgatc tcccgcttga ccttggtgcg
    14941 ggcctcgccc gagaagagcc gaacgacgtc ggggaacttc tcgtcgcgcc agcccgccgg
    15001 gtccacgaag gccctctgat accactcggt gatcaggccg acgatggctc cgccctggtc
    15061 gcgcgcctcg gcggggggtt cggcgtcgcc ggccgccacg acctcgacgc tgtcctggaa
    15121 cgcgagctgg acgacctcgt cgctgcctgc gggcgagccc gagcaagcgg ccgagccgaa
    15181 agctgccaga atcaggcata cggcgagcac gcgggaagag atgggacgag acaaggggag
    15241 catcggcccg ggaatgctaa ccggagggcc ggaggggggc aaggcacacc gtcgcgattt
    15301 tgccctgatt ccaggggttt ccgaaccacc ggccggatcg ggtgccgccg ctttgctcgc
    15361 agctagcacg cacggtagcc tactcgcgct ttcaacctgc cgaaggcctt cgcttaccaa
    15421 agaaaccggg aggtttcatg tcaacgatcc tcgctgcgga aggtggctcg atcaacgtca
    15481 tcttccgcga gctcgagaac aacatgctat gggtcatcct gggcatctct cttctggccc
    15541 tggccgttgc ctactacctg gtcaagggtg tcctgaggga gcccgaaggc acgccgaaga
    15601 tgatcgagat cgccaaggcg atccaggaag gctccgcggc ctacctgaac cgtcagttca
    15661 aaacggtcgg tctgttcgtc ggcgcgctgg ccgtgctgct gttcttcatc ctgccggtcc
    15721 cccagcagtg tcccggtggc gtccagatct cgctcagcga gacctgcgcg gacaattccg
    15781 agatggtgct caagctgggc cgctcgatcg cgttcgtcct gggtgcggcc ttcagcgcgc
    15841 tgaccggctt cgccggcatg tcgctggccg tgcgcggcaa cgtccgtgtc gccaacgccg
    15901 cccgcgagtc cggccttcgg caggcgctca ccatcgcgtt ccgtaccggt ggtgtggccg
    15961 gcatgttcac cgtcggcctg ggtctgctgg gcgccaccgt cgttctgatc atcttcaagc
    16021 gcgatgcgac gcaggtgctg gtcggcttcg gattcggtgg cgcgttgctg gccatgttca
    16081 tgcgagtcgg cggcggtatc ttcaccaagg ccgcggacgt cggtgccgac ctggtcggaa
    16141 aggtcgagaa gggaatcccc gaagacgacc cgcgcaacgc cgcgacgatc gccgacaacg
    16201 tcggggacaa cgtcggcgac tgcgccggca tggcggccga cctgttcgag tcctacgaag
    16261 tcacgctggt cgcctcgatc atcctgggtg ccgccgcctt cgcggaccgc ggcatcgagt
    16321 tcgcgatcgt cggggtcatg ttcccgctga tcgtccgcgc gatcggcgtc atcacgtcgg
    16381 tgctgggcat ctacatggtc aagccccgct ccgaaaacga gcacggcatg aagtcgatca
    16441 accgaggctt cttcgtgtcg gcgatcgtgt cggccgtcgc gatctacttc gcggccgcct
    16501 ggtacgtgaa ggacatccgc gtgttctggg cgaccgtgat cggtctgggt ctgtccgccg
    16561 cgatccaggt tctgaccgag tactacacct ccaccacccg caagccggtc atcgagatcg
    16621 ccgaggccag cgagaccggc ccggccacga cgatcctgtc gggcttctcg gtcggcctgg
    16681 agtccacggt gtgggcgctg atggtgattg ccgcggcgat cttcggcgcg ttcctgctgg
    16741 gcgacaacct cgccgagcgc ctgtacttca tctcgctgtc gggcatgggc atgctgacca
    16801 ccgtcggcgt gatcgtgtcg atggacacct acggtccggt ctcggacaac gcgcacggca
    16861 tcgccgaaat gtcgggcggt ctgggcgagc gggccgacga gatcatggcc ggcctcgacg
    16921 ctgtcggaaa caccacgaag gcgatcacca agggcatggc cattgcgacc gcggtcatcg
    16981 cggccacgtc gctgttcggc tcgttcttcg aggttctgaa agaggcgggc tcgcccgtca
    17041 ccgcgattcg cgtggacaac ccggacgtgc ttgtcggtct gctgatcggc ggctcgatcg
    17101 cgttcctgtt ctcgtcgctg gcgatccgcg cggtcagccg cgcggccggc caggtcgtgg
    17161 tcgaggttcg aaaccagttc cgcgagaacc ccggcatcat ggacgggacc gtcaagcccg
    17221 actacggccg cgtcgtcgac atctgcacca agacgtcgct tcgcgagctg atgacgcccg
    17281 gcttgctcgc cgtcgcctcg ccgctgatcg tgggcttcgc gttcaaggaa gaggcgctcg
    17341 gcgcgttcct cgccggcgtg atcgtgaccg gccagctgct ggcggtcatg ctctccaacg
    17401 ccggtggcgc gtgggacaac gccaagaagc tcgtcgagga aggccaccac ggcggcaagg
    17461 gcagcgaggc gcacaaggcg accgtcatcg gcgacactgt cggcgacccg ttcaaggaca
    17521 ccgccggtcc ggcgctcaac ccgctgatca aggtcatgaa ccttgtcgcg ctgctgatcg
    17581 ctccgatcgt cgtcgagcac gccggcgatc cgctctggcg ctacggcgtt ccgctcgtgg
    17641 cgggcaccat gctgggcctg tcgatctggc agtcgaagcg ccgcaagatc gacttcatgg
    17701 tctcggcccc ggcgggagtc ggttcggccg cgcccgtgac cgcgaccgca gccgtggtcg
    17761 agtccgcgat ggcaaccctg ccgccgccgg tcgagaccgc cgccgagcac cgcgaggagc
    17821 tggcggagat cgagggcgag gacgacgacc tggacgagcc ggttcctgcg cgccgcgcgc
    17881 cggccaagac cgcgacgcgc cgcccggctg cgaagaagac cgccgcgaag aagacggcgg
    17941 ccaagaagac gacggcccgc aaggctccgg cgaagcgctc ggcgtccaag acgacgtcgt
    18001 cgcggacccg caaggactag cagggccgga agagcaccac cgcgccgcgg gccgggcgag
    18061 aacgctcggc tcgcagcgcg tccgggtatc ggaggatcga tggggatctt cgaacggttc
    18121 ttccggcgac tcgcggtcga cgccgagtcg atccgcgtcg ccgaggtcgg ccgctgggcg
    18181 caagagatcc ccggcgtcgt ttccatcggc gaggcggagc ctcgcgccga ggtcaaggtc
    18241 gccggtgtcg tcgaacggct gcgggtgcgg cctcagaagg gcgtgccggc gatcgaggcg
    18301 gtgctggtgg acggcaccgg tatgtgcacc gccgtctggc tcggccgccg ctcgatcaag
    18361 ggcctcgagc tgggccggcg gatggtcatc gaaggccgtt tagccgtcaa ggacggcgac
    18421 cgtcagatca tgaacccgac cttcgagttc gccgaccaac gcgtcatcga agagcgcatg
    18481 aagcgcaaga aggactagcg ggttgcaacg agcccggtga cgacgacgta atggcacgtc
    18541 gccgcggaga gcacgaagac gtgccagatc tcgtggaagc cgaacgtcgc ggggcgcagc
    18601 accggttttc gcagcgcgaa caggatcgcg ccgaccgtgt acaggacgcc gcctgcgacc
    18661 agcagcgcca gcagcgcggc gtgcatgttg cgaacgatct gcggcatcgc cagcacgacc
    18721 gtccatccaa ggatcaggta cagcgcgaag ccgaggccgt cgccccatcg cacgcgtgag
    18781 gtcttgatgg ccgtgccggc cagcgcgccg ctccacgcga tgcccagcat cgtccagcgc
    18841 cacgcgcctt ccagcaccag caggctcagc ggcgtgtagg tgccggcgat cagcaggtag
    18901 atcgccgagt ggtccagcgc ctgcatccgc acgcgggcct tgtcggacca ggggagccgg
    18961 tggtacgagg cgctgactcc gaacagcccg gacagcgcga ttgcgtagat ggcggcggcg
    19021 gcgcggatcg agccggtcc
//
LOCUS       DATLFO010000118         5734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_121362, whole genome shotgun sequence.
ACCESSION   DATLFO010000118 DATLFO010000000
VERSION     DATLFO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121362"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VM841_04305"
     CDS             complement(<1..461)
                     /locus_tag="VM841_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HVL89440.1"
                     /translation="MLGRIAALAVESSEPTRKVFENFDEWMKISFYVLAGIASTAFLS
                     GAVVRVRKYRQGRKAGRIDKLGTRLAKAMHKVGSNKTVAKRNIKTGIAHALILWGFTA
                     LFIGTVILTIDYDVIRLAFGEDARFFKGNFYLGYSVILDTLGAAYVVALLY"
     gene            complement(471..1427)
                     /locus_tag="VM841_04310"
     CDS             complement(471..1427)
                     /locus_tag="VM841_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HVL89441.1"
                     /translation="MGDVYVIVEQRDGAVAPVTLELLGKAAEIAPSLGGQAVAVLLGG
                     SGIASSASQLAGAARAIVIAEDRLASYTPEAYKAALGEVLAGREPRLIMVGNTTQGMD
                     LAAGLSISLNLPLVAYSTGLVVEGSDVVATSRVYGGKIDAEVVLDSPGIVSVQAGSFP
                     PATGSASPEVEEVKSPSALDNLSTTFKALHEPAGGDVDITAQEVLVSVGRGIQSADNL
                     EVMQELADALGGALSASRPITDNGWLPKTRQVGKSGLNVKPKLYLCAGISGAPEHLEG
                     MRDAELIIAINTDENAPIFDVAHYGVTADLFDIIPAITERLK"
     gene            complement(1442..2212)
                     /locus_tag="VM841_04315"
     CDS             complement(1442..2212)
                     /locus_tag="VM841_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HVL89442.1"
                     /translation="MDIIVLIKQVPDLAEELEINSEGTDLDREYLTVVMNEFCEQALE
                     EAVLLKEAAGGTVTAVTMDGEEAENILFTAAAKGADNLVKVTGDFEDSALDSHALSAI
                     YAEVLAGRSYDVIMTGVQAVDDLDGQLGPLLASRLNLPHVSVVAGVTPDDEGLVVTQE
                     FGGGLTAELAVKTPCVLGVQAAKQSPRYAPVSKVRAMKQEKTIEDAEAPAVETPRLKV
                     RAMRTPESSSHAEMLTGSTDDVAEKVVALLRERGLVKA"
     gene            complement(2221..3306)
                     /locus_tag="VM841_04320"
     CDS             complement(2221..3306)
                     /locus_tag="VM841_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVL89443.1"
                     /translation="MAMTKEMLPLASERHVSPEWVRISMASAIALRFRSGRFTRAFEF
                     GGINLLLNYAEGCLSDCSYCGLARTRPGYYEEKSFIRVDWPLVQTDEFVDRLAKHEDT
                     LTRLCISMVTHGHAYQDTVDITRRIRAKVKTPLSILVAPPTLDREKIEHFKEIGVDMI
                     GVGLDAATEPLFIQHRTDVPQGGLRWKKYWEVIDDSREVFGPWKVNAHILVGLGETDR
                     ELCELFIRLCDREIFPYLFSFNPEPDSRLGVAPRAPLGRWRRIQLVKHLIEREGLRDE
                     HLVFDEAGALVRIDKPRAWLDEHVRDGEAFMTNGCPGPEGEPGCTRPYGSYRPSEPFR
                     DFPFLPLIDDVRGIEADLRLDELCPKE"
     gene            complement(3297..4460)
                     /locus_tag="VM841_04325"
     CDS             complement(3297..4460)
                     /locus_tag="VM841_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HVL89444.1"
                     /translation="MKTWRLISDDGASASRGLATDEALMRLYATGDPPLPTLRLYTYA
                     DHAALVGRFQDPRAELRLDVCRAEGIAVNRRPTGGGAILMGEQQLGVALVTSLRDPNT
                     PAHARAIIDTFGRGVLLGLKRLGIPAEIRGKNDIAVDGRKIAGLGIYVDANDGVLFHA
                     SVLAGLDIDLMLRILNIPAAKLGGQAADAVRQRITTVTAERGRACSTEDLRLCVEAGF
                     EDAFGIRVRPEPLDPAELAAARDVEAQRYGRDDWIYGRAAETPEGTAILKTPGGLLRI
                     ALTLGGDAIKDALIAGDFMARERAVAEIEAALKWAAADKGRVGEAVETGWSRGGGLDG
                     VAPGDLTEAVWAAVIDARARRGMTTGSCYYPPAPDEGSGDPTTSTREEAPAWQ"
     gene            complement(4457..5443)
                     /locus_tag="VM841_04330"
     CDS             complement(4457..5443)
                     /locus_tag="VM841_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVL89445.1"
                     /translation="MTLAFDTVAAPSRGWLASREHFGNRIDFFAPGLKRYETAEWKPK
                     NGRAFLPVSVTGSACALQCDHCSAKVLDGMISVSPDRDLFAIASDLKAKGTKGMLVSG
                     GSQRSGGVPLLAQIDQIARVKHELGMRVVCHVGYPDAETAAALAAVGIDGAMIDVIGA
                     DDTLRDVYHLDMTVADVERSIAHLADAGLRIIPHIVLGLHYGKFLGEQTALEMLSRYP
                     VWTLILVVLTPLVGTPMGHLPPPDTGEVIDFFHTARGRMDDTRVHLGCARPLGDIKVS
                     LDRAAVDAGFNGIAYPADGTIAYAESLGLEPRLFDGCCSMTWADFAEDPDSL"
     gene            complement(5440..>5734)
                     /locus_tag="VM841_04335"
     CDS             complement(5440..>5734)
                     /locus_tag="VM841_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89446.1"
                     /translation="LYLSPAGTIVAAGDELGTIEAQKFVGPLRAPVAGTVVQVNDRVL
                     ENPLVVSKDPYGEGWLLLIDPIEEPAGLIEGEAIHAWFAESLEEYKRQGSVAE"
BASE COUNT          898 a         1949 c         1949 g          938 t
ORIGIN      
        1 atgtacaaga gcgcgaccac gtacgccgcg ccgagcgtgt ccaggatcac cgaatagccc
       61 aggtagaagt tgcctttgaa gaagcgggca tcctcgccga aggccaggcg gatcacgtcg
      121 tagtcgatcg tcaggatcac cgtgccgatg aacagcgcgg tgaaccccca caagatcaac
      181 gcgtgcgcga tgccggtctt gatgttgcgc ttggcgaccg tcttgttgga gccaaccttg
      241 tgcatggctt tggcaaggcg ggtgccgagc ttgtcgatcc ggccggcctt gcgaccctga
      301 cggtacttgc ggacgcgaac cacggcgccg gaaaggaatg ccgtggacgc gatgcccgca
      361 agcacgtaga acgagatctt catccattcg tcgaagttct cgaagacctt tcgggtcggc
      421 tccgaggact cgaccgccag ggccgcaatc cgaccgagca tcgtcatctc ctacttcagc
      481 ctttcggtga tcgccgggat gatgtcgaac agatcggccg tgacgccgta gtgcgcaacg
      541 tcgaagatcg gcgcgttctc gtcggtgttg atcgcgatga tcagctcggc gtcgcgcatg
      601 ccctccaggt gctcgggcgc tccgctgatg cccgcgcaca ggtagagctt cggcttgaca
      661 ttgagccccg acttcccgac ctgccgggtc ttgggcagcc agccgttgtc ggtgatcgga
      721 cgagacgccg atagcgcccc gccgagcgcg tcggcgagct cttgcatgac ctcgaggttg
      781 tcggcgctct ggatgccacg tccgaccgaa accagcacct cctgagccgt gatgtcgacg
      841 tcgccgccgg ccggctcgtg cagggccttg aacgtggtcg agaggttgtc cagcgccgac
      901 ggcgacttga cctcttcgac ctcggggctc gcggagccgg tcgcgggcgg gaatgacccg
      961 gcctgaacac tgacgatgcc gggcgagtcg agcacgacct cggcgtcgat cttgccgccg
     1021 tagacgcggc tggtggcgac cacgtcggac ccttccacga cgaggccggt cgaataggcg
     1081 accagcggca ggttcagcga gatcgacagg cccgcggcga ggtccatgcc ttgcgtggtg
     1141 tttccgacca tgatcagccg cggctcgcgg ccggcgagca cctcgccgag cgcggcctta
     1201 taggcttcgg gcgtgtagga cgcgagccgg tcttcggcga tgacgatggc gcgcgccgcg
     1261 cccgcgagct gcgaggccga tgaggcgatg ccgcttcctc ccagaaggac ggcgacggcc
     1321 tggccgccga gcgacggtgc gatctccgct gcctttccga gcagctccag cgtgaccggc
     1381 gcgaccgcgc cgtcacgctg ctcgacgatg acgtagacgt ctcccatgat tgccgcctcc
     1441 cttacgcctt caccaggccg cgttcgcgca gaagcgcgac gaccttctcg gcgacgtcgt
     1501 cggtggatcc ggtgagcatc tcggcgtgcg agctgctctc gggcgtgcgc atggcgcgca
     1561 ccttcaagcg cggggtctcg acggccggcg cctccgcatc ctcgatggtc ttctcctgct
     1621 tcatcgcccg gaccttggag accggcgcgt accgcggcga ctgcttcgcg gcctggacgc
     1681 cgagcacgca gggtgtcttg accgcgagct ccgcggtgag cccgccgccg aactcctgag
     1741 tcacgacgag cccttcgtcg tccggggtca ccccggccac gaccgagacg tgcggcaggt
     1801 tcagccggga cgcaaggagc gggccgagct gtccgtcgag gtcgtccacg gcctggacgc
     1861 ccgtcatgat cacgtcgtag gagcggccgg cgaggacctc ggcgtagatc gcggacaggg
     1921 cgtgcgagtc gagcgcgctg tcctcgaagt cgccggtgac cttgacgagg ttgtccgcac
     1981 ccttggcggc cgcggtgaac aggatgttct cggcctcttc tccgtccatc gtgacggcgg
     2041 tgacggtccc cccggcagcc tccttcaaca agaccgcttc ctcgagcgcc tgctcgcaga
     2101 actcgttcat caccacggtc aggtactcgc ggtccaggtc cgttccctcg ctgttgatct
     2161 cgagctcctc ggcgaggtcg ggcacttgct tgatcaaaac gatgatgtcc atcgcagttc
     2221 tcactccttc ggacaaagct cgtcgagccg cagatcggct tcgatgccgc ggacgtcgtc
     2281 gattaacggc aggaacggga aatcacggaa cggctcgctg ggtcggtacg agccgtaggg
     2341 tcgggtgcag cccggctcac cctcgggacc ggggcagccg ttggtcatga acgcctctcc
     2401 gtcacgcaca tgctcgtcga gccacgcacg cggcttgtcg atccgcacca gcgcgccggc
     2461 ctcgtcgaag accagatgtt cgtcgcgcaa cccttcgcgc tcgatcagat gcttcacgag
     2521 ctggatgcgt cgccaccgtc cgagcggcgc gcgcggcgcg acgcccaggc gtgagtcggg
     2581 ctcggggttg aacgagaaca ggtacgggaa gatctcgcgg tcgcacaggc gaatgaacag
     2641 ctcgcacagc tcgcggtcgg tctcgcccag gccgacgagg atatgcgcgt tgaccttcca
     2701 cggtccgaag acctcccgcg agtcatcgat gacctcccag tacttcttcc agcgcaggcc
     2761 gccctgagga acgtcggtgc ggtgctggat gaacagcggc tcggtcgcgg cgtccagccc
     2821 cacaccgatc atgtccacgc cgatctcctt gaaatgctcg atcttttcgc gatcgagcgt
     2881 gggtggggcc accaggatcg acaggggcgt cttcactttg gcgcggatcc ggcgggtgat
     2941 gtcgaccgtg tcttggtagg cgtggccgtg cgtgaccatc gaaatgcaca accgcgtcag
     3001 cgtgtcctcg tgcttggcga gccggtccac gaactcgtcg gtctgaacca gcggccaatc
     3061 gacacggatg aaggacttct cctcgtaata gccgggccgc gtccgggcca ggccgcagta
     3121 cgagcagtcc gacagacagc cttcggcgta gttcagcagc aggttgattc cgccgaactc
     3181 gaatgcgcgc gtgaagcgtc cggagcggaa ccgcagcgcg atcgcgctcg ccatgctgat
     3241 gcgaacccac tctggtgaga cgtgccgctc ggacgcgagc ggcagcatct ccttcgtcat
     3301 tgccatgcgg gagcctcctc gcgcgttgac gtggtcgggt cgccggatcc ttcgtccgga
     3361 gccggcgggt agtagcagga accggtcgtc atgccgcggc gcgcgcgggc gtcgatgacg
     3421 gccgcccaga ccgcttcggt caggtcgccg ggcgcgacgc cgtcgagccc gccaccccgt
     3481 gaccagccgg tctcgaccgc ctcgccgact cgacccttgt cggcggccgc ccacttcagc
     3541 gcggcctcga tctcggcgac ggcgcgctca cgggccatga agtctccggc gatcaaggca
     3601 tccttgatcg cgtcaccgcc gagcgtcagc gcgatccgaa gcagcccgcc gggcgtcttg
     3661 aggatcgccg tcccttcggg tgtctcggcg gcgcggccgt agatccagtc gtcgcggccg
     3721 tagcgctgcg cctcgacgtc gcgggccgcg gccagctcgg cggggtccag cggctcgggt
     3781 cgcacgcgaa tcccgaaggc atcctcgaag ccggcctcga cgcacagacg caggtcctcg
     3841 gtcgagcaag cgcggccccg ttcggccgtc acggtggtga tccgctgccg gaccgcgtcg
     3901 gcggcctgac cgccgagctt ggcggccggg atgttgagga tgcgaagcat caggtcgatg
     3961 tcgaggccgg cgagcacgct cgcgtggaag agcaccccgt cgttggcatc gacgtagatg
     4021 ccgaggccgg cgatctttcg cccgtccacg gcgatgtcgt tcttgccgcg gatctcggcc
     4081 ggaatgccca gccgcttcag cccgagcaga acgccgcgtc cgaacgtgtc gatgatcgcg
     4141 cgggcgtgcg cgggggtgtt cggatcacgc agcgacgtga ccaacgcaac gccgagctgc
     4201 tgctcgccca tcaggatcgc gccgccgccc gtcggccggc ggttgaccgc gatgccttcg
     4261 gcccggcaca cgtcgaggcg aagctcggcg cgcggatcct ggaaccggcc cacgagcgcg
     4321 gcgtggtcgg cgtaggtgta gagccgcagc gtcggcagcg gaggatcgcc ggtagcataa
     4381 aggcgcatga gcgcctcgtc ggtcgcgagc cctcgggacg cgctcgcgcc gtcgtcggag
     4441 atcagccgcc aggtcttcat aggctgtcgg ggtcctcggc gaagtcggcc caggtcatcg
     4501 agcagcatcc gtcgaacagg cgcggctcca ggccgaggga ctcggcgtag gcgatcgtgc
     4561 cgtccgcggg ataggcgatg ccgttgaagc cggcatcgac ggccgcgcgg tcgagcgaga
     4621 ccttgatgtc tcccagcggc cgggcgcatc cgagatgcac acgcgtgtcg tccatgcgcc
     4681 cgcgggcggt gtggaagaag tcgatgacct cgcccgtgtc cggcggaggc agatgcccca
     4741 tcggcgtgcc gacgagcggg gtgagcacca ccaggatcag ggtccagacc gggtagcgcg
     4801 acagcatctc gagcgcggtc tgctcgccga ggaacttgcc gtagtgcaga cccagcacga
     4861 tgtgcgggat gatccgcagg ccggcatccg ccagatgcgc gatcgaccgc tcgacatcgg
     4921 ccaccgtcat gtccaggtgg tagacgtcgc gaagggtgtc gtccgcaccg atcacgtcga
     4981 tcatcgcgcc gtcgatcccg accgcggcaa gcgccgcggc cgtttcggcg tcgggatacc
     5041 cgacgtggca gaccacgcgc atgccgagct cgtgcttgac cctcgcgatc tggtcgatct
     5101 gcgccagcag cggcacgccg ccggaccgct gcgacccgcc ggagaccagc atgcccttgg
     5161 tgcccttggc cttgagatcg gaggcgatcg cgaacaggtc gcgatcgggc gagaccgaga
     5221 tcatgccgtc gagcaccttc gcgctgcagt gatcgcactg gagcgcgcac gcggagccgg
     5281 tcacgctcac gggaaggaac gcgcgtccgt tcttgggctt ccactccgcg gtctcatagc
     5341 gtttgaggcc gggagcgaag aagtcgatgc ggttgccgaa gtgctcccgc gacgccagcc
     5401 agccccggct cggagcggcg acggtgtcga aggcgagcgt cattcggcca ccgacccctg
     5461 gcgcttgtac tcctcgagcg actccgcgaa ccacgcgtgg atcgcctcgc cttcgatcaa
     5521 gccggccggc tcctcgatcg ggtcgatcag cagcagccat ccttcgccgt agggatcctt
     5581 ggacaccacg agcgggttct ccagcacgcg gtcgttgacc tgcaccacgg tgccggcgac
     5641 gggcgcgcga agcgggccga cgaacttttg cgcctcgatg gtgccgagct cgtcgcccgc
     5701 ggccacgatc gtcccggccg gcgacaggta cagc
//
LOCUS       DATLFO010000119         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_44768, whole genome shotgun sequence.
ACCESSION   DATLFO010000119 DATLFO010000000
VERSION     DATLFO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44768"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..984)
                     /locus_tag="VM841_04340"
     CDS             complement(88..984)
                     /locus_tag="VM841_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HVL89447.1"
                     /translation="MTLLLVDASSLIYRAWFALPDSMKGPAGEPVNAVHGFIDMLARL
                     VATRQPAGLVCCFDDDWRPEWRVALLPEYKAARVAEDGEEIAGPEEQIPVVRELLGVA
                     GIACAGAPGSEAEDAIGTLVAHSKKGPIGIVSGDRDLFQLVRDPDVSVLYPRKGVSDL
                     TVVDEAWIEAKYAIPGRAYLDFAVLRGDPSDGLPGVRGIGEKSAASLIRAHGSLPGVV
                     AAASAADAPKNGPLAKVAAQLDYVERATKVARIASDAPIGRVRVALPASLPAAFVEAA
                     EKAGVFGPAQRLAAAVASRKGG"
     gene            1109..>2130
                     /locus_tag="VM841_04345"
     CDS             1109..>2130
                     /locus_tag="VM841_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVL89448.1"
                     /translation="MAQTPKQPTSGTKRSRAVPRKKAAAKVARPPTPEEAPEFVTPTD
                     PWPVRVEYPSVGYWMKAAAGVIVVIALANMVVAISHVLILILISLILSIGVQPGVNWL
                     VLRGMRRGSAVAAIFVIGAVVVLGFLAMITPAIINQIGQLVEQAPERIREAQESNKFI
                     ADLNERFDLTGKLTNLADNLPSTALSLVRSFTSFVFNLLTVVILTLYFSTALPKMEIG
                     IARLLRRENREEFSTILEESTSLVGGYMIGNLVVSVIAGGVSFVALLLIGVPYPAALA
                     FWVALTDLIPTVGAILGALAAVLVAAFAGVPELVGTVIFFTIYQQIENYVIAPRVMTR
                     TVEMSAA"
BASE COUNT          343 a          753 c          702 g          332 t
ORIGIN      
        1 gtcccggact tgttcccgat ggcgcgacga aatccctccc ggatcgacca cggtcgcgga
       61 acaggctatc gcaaagcggg acgaacgtta cccgcctttg cggctggcaa ccgcagcggc
      121 gagccgctgc gcaggaccga agactcccgc cttctcggcc gcttccacga acgccgccgg
      181 caggctcgcg ggcaatgcga cgcgcacccg tccgatcggc gcgtcggaag cgatgcgagc
      241 aaccttggtc gcgcgctcga cgtagtccag ctgcgccgcg accttcgcga gcgggccgtt
      301 cttcggcgcg tccgcagcgg acgcggccgc gaccaccccc ggcaacgagc cgtgagcgcg
      361 gatgagtgag gccgcgctct tctcgccgat ccctcgcacg cccggcagcc catcggacgg
      421 atcgccgcgc aggaccgcga aatcgagata cgcccgtccc gggatcgcgt acttcgcctc
      481 gatccaagcc tcgtccacga ccgtgagatc gctcacgccc ttgcgcggat acagcaccga
      541 cacgtccggg tcccgcacga gctggaacag gtcgcgatcg ccggacacga tcccgatcgg
      601 acccttcttc gaatgggcga cgagcgtgcc gattgcgtct tcggcctcgg agccgggcgc
      661 gcccgcgcag gcgatcccgg cgacaccgag cagttcgcga acgacgggaa tctgctcttc
      721 cggcccggcg atctcctccc cgtcctcggc cacgcgcgcg gctttgtact cggggagcaa
      781 cgcgacgcgc cactcgggcc gccagtcgtc gtcgaagcag caaacgagcc cggcaggctg
      841 ccgggtcgcc accagacgcg ccagcatgtc gatgaagccg tgcacggcgt tgaccggctc
      901 gcccgcaggc cctttcatcg aatcgggcag cgcgaaccac gcccgataga tgaggctgga
      961 ggcgtccacc agaagcaggg tcacgcaccg caatctaccc cgccgcgcgg ccgggcgaag
     1021 ggacagggcg gcaacggcgt gagggcccgc ggcggggctt cgcggcacgc ggcagacccc
     1081 cggcgcgtcg tttacgcttg cgcgagccat ggcgcagacc ccgaagcagc ccacgtccgg
     1141 cacgaagcgg tcgcgggccg tgccgcgcaa gaaggcagcc gcgaaggtcg cccggccgcc
     1201 tacgccggag gaggccccgg agttcgtcac gcccaccgat ccgtggccgg tgcgggtcga
     1261 gtatccgagc gtcggctatt ggatgaaggc cgccgccggc gtgatcgtgg tgatcgcgct
     1321 cgccaacatg gtcgtggcga tcagccacgt cctgatcctg atcctgatct cgctcatcct
     1381 gtccatcggc gttcagccgg gggtgaactg gctcgtcctt cgcggcatgc gacgcggaag
     1441 cgccgtcgcg gcgatcttcg tgatcggagc cgtcgtcgtg ctgggcttcc tcgcgatgat
     1501 cacgccggcg atcatcaacc agatcggcca gctggtggag caggctcccg aacgcatccg
     1561 cgaagcgcag gagagcaaca aattcatcgc ggacctcaac gaacggttcg acctgacggg
     1621 aaagctcacg aacctggccg acaacctgcc ttcgacagcc ttgtcgctgg tgcgatcgtt
     1681 cacttccttc gtgttcaacc tgctgaccgt cgtgatcttg acgctgtact tttcgacggc
     1741 actcccgaag atggagatcg gcatcgcacg cctgctgcga cgcgagaatc gcgaagagtt
     1801 ctcgacgatc ctcgaggagt ccaccagcct ggtcggcggc tacatgatcg gcaatctggt
     1861 cgtgagcgtg atcgccggcg gcgtgtcctt cgtggccttg ctgctgatcg gggttccgta
     1921 cccggccgcg ctcgcgttct gggtggcgct gaccgacctg atcccgacgg tgggcgcgat
     1981 cctgggcgcg ctggccgcgg tactcgtcgc ggccttcgcg ggcgtgcccg agctcgtcgg
     2041 caccgtgatc ttcttcacga tctaccagca aatcgagaac tacgtgatcg cgccgcgcgt
     2101 gatgacgcgc accgtcgaga tgtcggcggc
//
LOCUS       DATLFO010000120        29795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_44794, whole genome shotgun sequence.
ACCESSION   DATLFO010000120 DATLFO010000000
VERSION     DATLFO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 29795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44794"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VM841_04350"
     CDS             complement(<1..1173)
                     /locus_tag="VM841_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL89449.1"
                     /translation="MTRVPRSDQVVFARFVGAHVSGVAGDTLVAIALSGTLFFNVSID
                     QARSKIGLYLLLTMAPFALLSPVIGPFMDRHTGSRRLAVIASMIGRAAVCIAMASTLD
                     TLLLYPLAFAVLVMGRAQSVARAALVPSLVDNPKRLVFANARLAKAAVAGGAIVAMPG
                     IVVLKLGNESAVLVLAAMMYGLGLIASFALPRPPPDRPGLGIEPLMHLGKIRHAAEVQ
                     TLVRGLNGYLLFLLAFGLRGAGISDAGFAFVLGFAGLGGFAGAVIVPKLRRSGNEEWI
                     TATALFLGGGVSLIAGRSFGLDLAMVLAFTVGIVGSATRLAFDSIVQREAPEAVRGRV
                     FARFETLFQIGWVVGAALPVAFKIPIAGGLVASTAVYAAVALWFLAGIGQTGRARIC"
     gene            complement(1170..1388)
                     /locus_tag="VM841_04355"
     CDS             complement(1170..1388)
                     /locus_tag="VM841_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HVL89450.1"
                     /translation="MTEHEIAALAKRAGGASTLVAPTRRAEVEGMTDAQIVAYLVQDP
                     KRLRRPIIDTGDAIHLGFSKAVREALAE"
     gene            1565..2257
                     /locus_tag="VM841_04360"
     CDS             1565..2257
                     /locus_tag="VM841_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89451.1"
                     /translation="MEPIVAARGLRKVYHGGTDVEALKGIDLDVAPGSMTAIVGPSGS
                     GKTTLLNCLSGLERIDGGSVIIGGQNLASLDDDRRTDLRARTMGFVFQAFNLLPVLSA
                     AENVELPLLLQGVSAREARALAVAMLEQVGLGPRVSHRPEQLSGGEQQRVAVARALVH
                     SPVVVWADEPTGNLDTESTEAIMSLLERLNGEGQTIVMVTHNATLAARANRIIHMRDG
                     LIEGVETPSARS"
     gene            2259..5087
                     /locus_tag="VM841_04365"
     CDS             2259..5087
                     /locus_tag="VM841_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVL89452.1"
                     /translation="MAAAAIPLLLFAIAFGLVAVVGRRNPMLARMAAREATRRRGQTA
                     LVVGGLMVGTATITAALIAADSVGDSTTDAFAYRNWGYVDLTVMADGNRFFDAGVAAA
                     LSADETVRRVTDGIAAGIEVVGSASDLSTRQGTSGVNIVGFDPAAQKPFGSFVLTDGR
                     RTTGEDMPGGWVLISRTLADKLDAKPGDRLRVRAVAAGLQGAAGTELALFGIARSEGP
                     GAYTLGSAVFAPLPTVQSILGTAQINVVRLSAPGGIRDADDAARAAAAPVRAAVERVA
                     PSLEVETAKLRAVDFAAENTMFLRAMLTGMSAIVVAAGAALVVNITGMLAEERRARLG
                     VMRALGLRRRKVVALSVIEGAMYALAAGIAGAIVGVAAGRLVASRFGRAFAEFAGEDF
                     DFEFFFSLRPETLATGFAVGSVLTLIVIAIASRRTARMTITAAIRDLPEPPPEKPRQQ
                     WPRNARLAVGALAGLFLVSQSSIFPRMAGAELLTLVASAFVRPKLNPRAHGTLTGLAM
                     AAVAFGLIAGQAPEGDPAAFFGGFVIAMLSAVFGLTILVTANLHVVERGVSLAGRAMS
                     RLIAIVRPPIAYLSRRPMRTGLTTGVFALIVAMLTLFAVFLVIFQPSLEQFSAGYDVR
                     LLSTGSPDVTIPDQVRPDVERVATIPTRGYVGPIEGSGPFSSADRAFVPLLVAPSDAP
                     TNPPVRLFERSRRYSSDEAAWQAILRDPTLVVSNFGEPDAKITLRGQNGPVTFTVVGS
                     HQFGLLDGVFASPEALKPFESAPLGASLLIDLRDGADARTAARTIERELFGVGVDADS
                     TQDLYDTASRGSRAFFSTIDVLMRLGLVVGILSLGIVAMRVVIERRQLIGMLRAIGYK
                     RKHVLAGLMAEAAATATIGVAVGATVGLLMGYIFSTQQEGAAGFGVDWGSFFGVLGVV
                     YVAVTIVALGPAWRASRLPPAEAVRYSE"
     gene            complement(5092..5481)
                     /locus_tag="VM841_04370"
     CDS             complement(5092..5481)
                     /locus_tag="VM841_04370"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HVL89453.1"
                     /translation="MDAPDSMLHDPEFWALGPRPAPQDIATELARTMEAGRFVRRDTT
                     YVQTVYTVALSEHDLARFDGRKRELVATLKDTLAWWAREQGYTVPGQLRVRLIADDQL
                     EAGQLTVEAGLRREEAAKLGRIRSGTE"
     gene            complement(5486..6205)
                     /locus_tag="VM841_04375"
     CDS             complement(5486..6205)
                     /locus_tag="VM841_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HVL89454.1"
                     /translation="MRFDDTLDISRAMVIYAHPDDAEFGASGTVAKWVRAGVEVTYVM
                     ITNGGSGSSDPAMTREKLVEIRRAEQEAAAKVLGVAHVEFLGFEDGYLVVNEESRRAV
                     ARQVRRYKPDVIIGLDPTLRYTDFYTNHPDHIAAGDLVMFSINPDASTRLMFPELVEQ
                     EGLEPHKPKMLLLMNWGAEPDHVEDISSTIDVKIEALSLHASQVENMDEIEQWVRQRA
                     GEIGAPTGYAAGEGFKVVRFE"
     gene            complement(6230..6667)
                     /locus_tag="VM841_04380"
     CDS             complement(6230..6667)
                     /locus_tag="VM841_04380"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HVL89455.1"
                     /translation="MRYFAYGSNMDLVQMDGRCPGACVVGPARLPGYRLGFVTDETGY
                     DGGVATIVPDPASEVYGVLWEIDDTHLAALDEWETYPVAYRRHTITVHAPEPVEALVY
                     VANFSSPEPPSDRYLDVILRGLRDNGIGPEYASLIRRLAGRDG"
     gene            complement(6676..7230)
                     /locus_tag="VM841_04385"
     CDS             complement(6676..7230)
                     /locus_tag="VM841_04385"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HVL89456.1"
                     /translation="MSDIDPEERESILEDLEDLEAMRAVFEPRGYKGVVIVCSECGED
                     HYYEWEMLKESLEHMLETGEPRMHEPAFEPSPDEYVGWDYARGYLDAITEADADEMEP
                     PPEPPIPEASAWLGDRTRCAYCRGRLPSKAWAEWSYCPFCQASLAPLRLVDQLASRGW
                     DEDDIVALMDQCGFEPPLEKGEEA"
     gene            7429..8622
                     /locus_tag="VM841_04390"
     CDS             7429..8622
                     /locus_tag="VM841_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89457.1"
                     /translation="MSGVQGLDSGGIPRWITGLVFAAIVAALAVVVPAQVRAAHTPSP
                     SNLCVCFRMSPTAPKLTWSDTAGSATIQGYILQRSRTADFTSAVIEIRIARNTFDFID
                     ETIEFNTRFYYRVASYLGSDRSGWSNVVSATNGNGTYPTKPATPSDLTLSNEGGGLRV
                     SWSNPATNETGVWVQRSSDNGATWEYRASLAMNSTSYHDNDVPAGAPMYRVATFNSAG
                     ASYSYSVQYVNQNAPGVPTVGPIAPSIPAMSIPGFPSVGIPSVGFPTVPGFSVPPGLI
                     PSNPTAIASPWLNMLPSNPTAIASPWLNLIPSNPTAIASPWLNMIPSNPATLVPTVRD
                     PLTYRVWIGLRFGSPQNQPVGVWILPATCFGFGCPPPSMQPPSNCIGFRGALELCYLA
                     DATLN"
     gene            complement(8635..9651)
                     /locus_tag="VM841_04395"
     CDS             complement(8635..9651)
                     /locus_tag="VM841_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL89458.1"
                     /translation="MKYGMTVGGPLELIGPVARRAEAAGYESIWVAETARTAYIQAAV
                     ALQATTTATVGTSIALAFPRSPVITAMTARDLAELSGGRFILGLGTQVKRVNELRYST
                     PFEHPAPKIREVIEVCRKVWDAYAGEPISHEGRFYTVTMPPFPGGSPPPSPIPVGVAA
                     VNPLMLSLAGEVCDVYLGHPFSSVKYFDEIVRPAIDAGAAKAGRKPSECRVAQGAITS
                     IAETTEAAIAGAKLQIGFYGTTRTYAKIFEIHGWGHMTVALREKFAKGDMQGMIDTVT
                     DEMAEVYAIFGTPDEVRDKVKRWEGRADELILGPPWASPDMTRTEETFEAILETFAPA
                     AGRS"
     gene            9722..10978
                     /locus_tag="VM841_04400"
     CDS             9722..10978
                     /locus_tag="VM841_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVL89459.1"
                     /translation="MTIAETFAEFAADITLARLPDDVVAAVRRRTLDLIGCMLASAAD
                     GSAAPVVDAVHAWAGSRETGVAGYDFRAPAPQAAFANGTLAGALVHDAVHVPSLAPLD
                     AAVIPAALAVAEEAGRDGASLLEAIAIGSEIAARIAVAEGTVALPAGAAAAAARLWGL
                     SERETAAAIALAATSATPASEHRARLRRGWAAMTGVIAADLARRGVAADAGVPALDVP
                     LRDAGERWEMRSVAIKRYPAGLWLHAPMDAAAGLGLKAGDIDEVLCALPPGAAAVVCE
                     PRGEKIRPVTDDEARFSLPFGVATAIVGGRSPSEMFSEDARTDRRVLALAARVGYEEN
                     AALDPTGARVRIMLRDGRTRDAAQQACDGSPARELSDEDLRAKFTANARRRLDQPATA
                     KLATQIMRLEDLDTIDEIVALAQGDG"
     gene            complement(10975..11937)
                     /locus_tag="VM841_04405"
     CDS             complement(10975..11937)
                     /locus_tag="VM841_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HVL89460.1"
                     /translation="MHLGALVPQGWKHDLDGLDPQAAFAKMIEVAQRCESLGLDSIWL
                     YDHFHAVPWPAEPGVPVFECWTGMMALAMRTERVRLGQMVTCTPYRNPGYLAKIAACV
                     DVASGGRLEFGLGGGWYEEEYVAYGYGFHEPRERLGHLRDTVNIVERMWRDERATYDG
                     TYVSVKDAVNDPKPLQSPRPRVWIGGGGEQITLRITAGHADWANFMGDVDTFKRKRDI
                     MRGYAEEMGRDPASIKLSIHCEMLVAPDEPALGALTERHPSIFGRPEEQRRATNLIGT
                     TQQVIDKIAAYADLGCEAVIPWFPDYPDSGSLEIFATEIRAAFA"
     gene            complement(11953..13356)
                     /locus_tag="VM841_04410"
     CDS             complement(11953..13356)
                     /locus_tag="VM841_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVL89461.1"
                     /translation="MADVYDLVIVGGGVAGLCAALSAPEGASIAVIDKGEAASGSSPL
                     AQGGLAAAVGPDDSAELHAKDTMAAGAGLCDPDVVADICGEGPAAVAWLQALGCDFDR
                     GDGDGLDLAREGGQSVPRSVHWRDATGAEIVRALRAAARGRAQRMDARARAIAMEDGR
                     CAGVVTDQGTVLGRATLLATGGAGGLWASTTNAPGATGDGTALAMAAGAAIGDLEFMQ
                     FHPTALAVGGAQQRVLLTEALRGEGATLIDADGERFVEEMAARHIVAKAIIDAGEAWL
                     DARGVPYLEDRFPTIVEGARRHGFDPVTQPLPVYPAAHYFIGGVAARADGATSVDGLF
                     AAGECATTGMHGANRMAGNSLLEAVVTGRRVGQGLGGLPAPSGRLEDGPEPLGDLPAS
                     LPAIMWQRCGPVRDEEGLKHGLVALDEISGSWHARLCVTIVAAALERRETRGVHVRSD
                     WPLPDVGLGGRSMIVAR"
     gene            complement(13400..13717)
                     /locus_tag="VM841_04415"
     CDS             complement(13400..13717)
                     /locus_tag="VM841_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89462.1"
                     /translation="MSEHDRPVDWDRLLIEEQLQDYSRRQREPDPMGKRALYSVGARG
                     TGPMGTLTLECSSCKRESPVRLRELPRLAMPFWMAVPRPYPAFLKCPGCGRRTWLKPH
                     WRL"
     gene            complement(13714..14487)
                     /locus_tag="VM841_04420"
     CDS             complement(13714..14487)
                     /locus_tag="VM841_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL89463.1"
                     /translation="MARVIAVANQKGGVAKTTSVIALSAAIAEQGPRVLALDLDPQAC
                     LTFSLGLDPDTIDTSIHDVLVGRVPIGKVITQLDEVDLVPANIDLAGAEAYLLTRTGR
                     EYTLNAALEDVRGHYDFIVVDCPPSLGVLTINALTAAGEVIIPMQCETLSHRGVGQLL
                     ATIDDIKRLTNRNLEVTGILPTMFDARTAHSKDVLDDVARRYRLPVLEPAIRKSIRFA
                     EAPRTGRSMLKFAPSHPGTRAYREVARKLTGLPQQDAAS"
     gene            14640..16358
                     /locus_tag="VM841_04425"
     CDS             14640..16358
                     /locus_tag="VM841_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL89464.1"
                     /translation="MPEFDPTILPGASSEAPHGGRLAAAALREGGVDTIFALCGGHIL
                     GLLDGCVDEGIRVIDVRHEGAASLAAEGWALATGRTGVAAVTAGPGFGNALTGFIDAG
                     VWTLPMVLLAGRTGVAQQGRGAVMDVDQRGIVAPVAKWSATCLATPRIPAVIAEALHR
                     ARAGKPGPVYVEIPQDIFMASAEPDSPRAAAGVPSVAPAPAGDPAEIARALDVLQRAE
                     RPLLIAGGGAFWSDAGDALARFAETASIPVATTSAARGLLPDSHEWCLGSLVHAGVAA
                     LAADVVVVAGSAFNANMAFGQPPLIGPAVTVVQIDIRPEAIGGNRAADIGIAGDAARV
                     LGGLADGWTKTPDGRDAWLAQARELAGHGLAAWDAQIDGYTGPGIHCGAVARTTARVL
                     RETCGGDVTFVADGGDALSWGLAYGYAEGPGRMMTTTTALGTLGVGMPFAIGAAAARP
                     GEPVVLLTGDGSFGLTAMEVDTAARHGLPVIAIVSNNGSWGDVVHEQRAWYGRETASA
                     LRGARYDVLGEALGARGWLVESFEAFEPALREALASGGPCVIDVRTDPEILSDLLRNL
                     SSMNLM"
     gene            16383..17114
                     /locus_tag="VM841_04430"
     CDS             16383..17114
                     /locus_tag="VM841_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVL89465.1"
                     /translation="MSDLITYDINGAVTTITMDDGKVNALSPAMLAGINEALDAAEKE
                     RATVVLAGRDGRFSGGFDLTILRAGGDEAIGMLRGGFELSERLLSFPTPVVIACTGHA
                     IAMGSFLLASADYRIGAAGDFKIAANEVAIGLTLPLAAIEILRQRLTPAAFSRATLLA
                     ETFTPDDAVAAGFLDHVAPPADVLAIAQTVAAGMAGGLHMDAHAASKLRVRAAALEAI
                     RAGIDAEFPDGEGPATAIIQRGRSG"
     gene            complement(17115..18302)
                     /locus_tag="VM841_04435"
     CDS             complement(17115..18302)
                     /locus_tag="VM841_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL89466.1"
                     /translation="MSGRGGAAAPRLVTPLFVRITLTSFAYFASYGMTLPVLPKFVDG
                     PLGGGGLEVGLVGSAFAFSSIVLRPIAGRVGDARGRGILMAGGAAIVATATLPTGYAG
                     AVWLAFLFRFSAGFGEAGFFTGAASAINDLAPEERRGEAVSYFTLSLYSGLALGPLAG
                     EAIMKAAGFKAAFLAAGALAGVAALATLRLPDTRPDKDGPQGRGPIFHRAAFGPGSIM
                     TTSVIGFAGYAALMPLYARNLGMDAAPIFLAYSVVILVLRLAGARVPDRFGARRTATT
                     ALMVSASGLLLVFAWRGSLGLVLGSMLFATGQAFVFPSMLTLAVRGAPARERGSVVGT
                     FTAFVDLGFLAGPLLMGFLADLYGYRGSFLGGSIVGFAGLVWLLARIRAEHHAAHPEP
                     GPI"
     gene            complement(18299..19465)
                     /locus_tag="VM841_04440"
     CDS             complement(18299..19465)
                     /locus_tag="VM841_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL89467.1"
                     /translation="MGQIRISAKAASFTESVIREMTRVAMQHGAINLAQGFPDFSCPP
                     ELKEAAKAAIDADVNQYAITWGAQRFREAIAANFERFSGVPVDPNRQICVTCGSTEAM
                     IAAALAIVDPGDEVIVFEPFYENYGPDAILSGAVPRYVTLHEPDWTFDEAELAAAFNE
                     RTRAIIVNTPNNPTGKVFSREELQFIASLCQKWDVICFTDEIYEHMVYDGREHIPPMT
                     VPGLEDRSVTINAMSKTYSVTGWRIGWTISSPELTDGIRKVHDFLTVGAAAPLQEAGV
                     VALNFPDAYYKSLREEYLERRDLMTGILEDAGFKVYKPSGAYYIMTDISAFGWDGDDV
                     EIAKRLAADPGIATVPGSSFYADPSLGRTKLRFAFCKRLETLQAAAERLAMLRA"
     gene            19629..20936
                     /locus_tag="VM841_04445"
     CDS             19629..20936
                     /locus_tag="VM841_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HVL89468.1"
                     /translation="MHSPGGPSPWQGLLHRRPETGGVAIMMAIMMLVSLVMAAFAIDL
                     SNARSDRTLADSAADSGALAGAQSLPDPALARLDAREYATAALTGGETGTGATVTVTT
                     PYTMSGNAYPAHRLVNVRVCWNSPTGFAAAFGIPSVNVCGVATAFKTNGTPCGLCVLG
                     TTGRTLSIAGNGTLRVTGADVHVNSNGTPAAGVEGTATGGVTTDGVIRILGTYQQPSP
                     GTFSPTPITGVPAIPDPLSTIPVPSVAGPYRGSVAHTGPENLTLQPGIYDSILSESRG
                     VLTFSPGIYVIRTGLTLQKSAQAPVPSVVGDGVLLYFGCSLYPTPCLSGGQTGAGISM
                     SGSTTFRISGMTSGPYTNLPIFFDRNNTASMTILGGVLNRIDGVVYARSAHYSMAGSS
                     GSISHNGAVVVGRTTTTGSSNFTVAFTTDYAHPVLGRHTFLVE"
     gene            21052..21687
                     /locus_tag="VM841_04450"
     CDS             21052..21687
                     /locus_tag="VM841_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVL89469.1"
                     /translation="MTDIVRFTNNYSDHSTEAGYQFEFYCMRCGNGYKSGFNASALGI
                     GNKVVRGLGGLLGGRLGGASYAGHELKDLTESQAKDKALRKAVEEVSPLFNQCHRCGS
                     WVCKDICWNDEAQLCVNCAPKLEQEIAGMQQQRRLQQVSEKINESDYASDVNVERMQV
                     ALCPHCGAEAQHGAKFCGECGQTLAVQVQCGRCGTTAAPGTKFCPECGNGL"
     gene            21687..22982
                     /locus_tag="VM841_04455"
     CDS             21687..22982
                     /locus_tag="VM841_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89470.1"
                     /translation="MAAVPGQVAVGGGDPVACTVEAAEDGVRVGGGVQAFIAYDDIDD
                     LLDDSYTLRIAVEDGSRYDFGMLGRAYGQIAADIVKRRDASLEKNLLLRGVNLRDTYP
                     GMLLGEDALQPVGATFRLYEDLLVVRPDHGVMFGVPFSAVDAVDWDAELYQAHIRLDD
                     GTTVTIGKLAKRSEEFTGELRRLLDALAERTRATLSGLLPDVSPGTITALAGAMRDGR
                     AVQKRTVDAIDPGLWPKLATAVLGSRRDSYERLAAMTPDGWAAFGVKALKAAASDEAA
                     EEQPPAAEPEDPETGPEAERTVPGGGENRLWFFCPLSRDGVPINAVASEVTSESGHAT
                     YVFRLMEPERFASLRGDELADEIGRAIGRLNRALLLLNFRREPLYASEDEIRSGRLTR
                     YRVALRLLPYLRWARESFLGRAAHTAGWDAAIDRILEKA"
     gene            23070..23585
                     /locus_tag="VM841_04460"
     CDS             23070..23585
                     /locus_tag="VM841_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HVL89471.1"
                     /translation="MRGIQPSAARGQRGASAVEFAIVLPVIALMLFGIIDFGVVLNNQ
                     NTVRAAAREGARLGAVAQFTGGAGCTIVGTVSSTETRGLMCLIKERNHPIGEAARVRV
                     IVPSPYRAGEPLVVCVQTKMSSLTGFTSPFFESKTVTAKTTMRVERTSSGDFASGQET
                     APPGGSWTWCT"
     gene            complement(23593..24300)
                     /locus_tag="VM841_04465"
     CDS             complement(23593..24300)
                     /locus_tag="VM841_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89472.1"
                     /translation="MKRPTLRLGAGILMSAMLALPVVFVPREAHAATYTVLARDDGFF
                     QTSIEIRPGDSVIWKNDGQITHTIDAQSGAPQPSPRQLAPKESTNPLTLSTLGNYKYR
                     LDGRSLEGYEMTVKVTNNPSSPSPSPTATGTITITTTPTSTPTRRRTSSPTPTKTTSS
                     PSPSPSPSTPTPTPTTPTPTPTPTEPTDSASPIPSFDDDTTGSPSSGNSQAIIAILGV
                     LVLGALGFVVYRRTLSG"
     gene            complement(24491..26284)
                     /locus_tag="VM841_04470"
     CDS             complement(24491..26284)
                     /locus_tag="VM841_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HVL89473.1"
                     /translation="MTETVVSLFLKTVAERGDAVAFKVKRGGEWQSISWIEYASDVQK
                     AANGLLSLGLGAEDKVAILSLNRPEWHMADVATMALGGVTVPIYVTNSPPQVSYIVGH
                     SEAKVIVCENAGQVEKILKTRNELPELKKVVVIDTDGATLDGFVMSWADLLAAGDEFG
                     KQNAGRFEEQVKAIKPEQLATLVYTSGTTGPPKGAMLTHDNIVWTCESLNKALEGQPD
                     DRSLSYLPLSHIAERVTSHMMQIWRGSETWFAESLDTLREDLQACRPTIFFGVPRVWE
                     KFYAGIKNLLDNLPEEQRVKAEQAVQLGLKRVEATQAGSSLPDELEAAYQQADAQLFA
                     IARAALGLDQARAMISGAAPINPEVLRFFHAIGLPVAEVYGQTEDCGPTSINRPDAIK
                     IGTVGPAIDGVEIKIAEDGEILVKGGNVGPGYYKNPEATTELIDEDGYMHSGDVGELD
                     EEGYLRITDRKKDLIITAAGKNIAPQVLENMLKHSPWISQAVVIGDARPYLTAILTLD
                     QEKVTAFAEQKSIPHGDFAELARHPDILQLVETSVQEVNEQLSRVEQIKKWTVLERDF
                     LQEADEMTPTMKVRRRAISDKYTDLIESMYA"
     gene            complement(26372..27481)
                     /locus_tag="VM841_04475"
     CDS             complement(26372..27481)
                     /locus_tag="VM841_04475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL89474.1"
                     /translation="MRILLVTNDYPPRVGGIQVYLENIYRRLAARHEITVVAPPHPRA
                     AAWDAQQPYEIVRHGPRVYWPRTGLQRRVADLAGRHDVVAFGAVVPMNWMAPKLGRPY
                     VIHTHGFEVALTRLPGARAALRRVCADARVVTVVSEYCRRFIEPVVGAGPRIEMLKTG
                     VDLEMFHPGVDGSEIRKRHGLDGRPVLACVSRLVPRKGQDTIIRAMPAIARRVPDAAA
                     LIVGGGPHRAALEKLAFTLRVADRVIFTGETPFAELAAHYAAGDVFAMPCRSRFADLE
                     VEGLGLVYLEAQATGRLAIAGDSGGAPEAVIPGETGLVVPGGDPAALAEAVAGLLADP
                     ARAAAMGEQGRAFVEANHHWDRVAERYERMLEESV"
     gene            complement(27478..28716)
                     /locus_tag="VM841_04480"
     CDS             complement(27478..28716)
                     /locus_tag="VM841_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL89475.1"
                     /translation="MTAPRSRLRLILIAAALAAAGFALAAPAIGAQPDEPRVTDYFTQ
                     EQIERSRDYTGTRYVLGFSSLVLGLILAGIVGLGPAARALGGWAERVSGGRWPLTALI
                     LAAVVTLIPVLATLPLAVARGYWHERRFGLSTQSLGGFLADVGKGAGVQVVVAGVVAL
                     AFFFIVRRLPVTWPLAAAAVMVALTVVMVVVYPLVYEPLFNKFTPVDPATRERIVALA
                     DRAGVTVDRVLVADASRRTKRLNAYVSGLGATKRVVLYDTLLERSPPDEVDLIVAHEL
                     AHVVHRDVLRGTILGSAGAIGAVVLVWLLTGRPGVLRFAGASGPGDPRLIPLLAFTMA
                     VATLVTLPLANGFSRSIEADADRFAVRLTGDVQTAIRTEVSLARENIADLRPHPLIRW
                     MFFTHPPVMERIEIALEAAP"
     gene            complement(28727..>29795)
                     /gene="sepH"
                     /locus_tag="VM841_04485"
     CDS             complement(28727..>29795)
                     /gene="sepH"
                     /locus_tag="VM841_04485"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HVL89476.1"
                     /translation="IKLRLIGSSADLKHLVYSNKRTGKKGSHLVAIDDDLFDVLEDVV
                     RRRRRGEGIDDEEDHTPRPVAPRVESKLPPREVQRQLRAGRTPEQVAKAADVPIAWVE
                     QFLVPVLYERQGMVREAQRAWLSKQRLGESGLPLGEAVEANLRSRHVRLTDEEMTNAW
                     DATRVEGQPWTITLRFRFRGREQRAMWKYDPTRRSVTAANRLGADLGWVAPGKRLRTG
                     ERTSRFVSPEPPGRRAAAEAPEKPARKRPAKKASAKRTSAKKASARKAPAKKRTPAKK
                     RTAAKKRTAAKRTAAKKRTAAKKRTAVRRPAAKRPAKKRTAAKRPAPARRPAKKRTAA
                     KRRSTRAPAKKRTAARRRTRR"
BASE COUNT         4407 a        10390 c        10471 g         4527 t
ORIGIN      
        1 acagatgcgg gcgcggccgg tctggccgat gccggccagg aaccacagcg cgaccgccgc
       61 gtagacggcc gtcgatgcga ccaggccgcc ggcaatggga atcttgaacg cgacgggaag
      121 cgccgcgccg acgacccatc cgatctggaa cagggtctcg aagcgcgcga agacgcggcc
      181 gcgcacggct tcgggcgctt cgcgctgcac gatggaatcg aacgcgagcc gcgtcgcgga
      241 cccgacgatc ccgacggtga acgccagcac catcgccaga tccaatccga acgatcgtcc
      301 cgcgatcagc gagacaccgc cgcccaggaa caaagcggtc gccgtgatcc attcctcgtt
      361 gccgctgcgg cgaagcttgg gcacgatcac agcgccggcg aagccgccca gtccggcgaa
      421 gccgagcacg aacgcaaagc ccgcgtcgct gatgcccgca ccacgcaaac cgaacgcaag
      481 caggaacagc aggtagccgt tcaggccgcg aacgagcgtc tgcacctcgg cggcatgccg
      541 gatctttccc aagtgcatca gcggctcgat gcccagaccg ggccggtcgg gcggcggcct
      601 gggcaacgcg aacgaggcga tcaggccgag gccgtacatc atcgccgcca gcaccagcac
      661 cgcggactcg ttgcccagtt tcagcaccac gatgccgggc atcgccacga tcgcgccgcc
      721 cgcgaccgcg gccttggcca gtcgcgcgtt ggcgaagacg agccgcttcg ggttgtccac
      781 cagcgacggg accagcgcgg cgcgcgccac actttgggcg cggcccatca ccagcaccgc
      841 gaacgccagc ggatacagca gcagcgtgtc cagcgtggat gccatcgcga tgcacacggc
      901 cgctcgtccg atcatcgacg cgatgacggc caggcggcgc gagccggtat gacggtccat
      961 gaacgggccg atcacgggcg acagcagcgc gaacggcgcc atcgtcagca gcaggtacag
     1021 cccgatcttc gaacgcgcct gatcgatcga gacgttgaag aacaacgtgc ccgacagcgc
     1081 gatcgcgacc agcgtgtcgc ccgcgacgcc ggatacgtgc gcaccgacga agcgggcgaa
     1141 gacgacctgg tcgctccgcg ggacgcgcgt cactcggcga gcgcctcccg gacggccttg
     1201 gagaacccca gatggatcgc gtcgccggta tcgatgatgg gacggcgaag ccgcttcgga
     1261 tcctgcacca ggtaggccac gatttgggca tcggtcatgc cttcgacctc ggcgcggcgg
     1321 gtgggcgcga ccagcgtgga cgcgccgccc gctcgcttcg cgagcgcggc gatctcatgt
     1381 tcggtcagcg gctccttgaa gaagtttcgc tcggcgggct tttcgtgcgc gagaaactct
     1441 cgcgcgtcgc ggcagcccgt tcagccgggg cgcagataca gggtgatgtc gcgtgccatg
     1501 cgtcgcatcg tacctcccgg caacgaccgc tcgggtatcg tgaggtagga ggaggcgagg
     1561 cggattggaa ccgatcgttg cggcgcgcgg attgcggaag gtctaccacg gcggcactga
     1621 cgtcgaagcg ctcaagggca tcgatctgga cgtcgcgccc ggctcgatga cggcgatcgt
     1681 cggcccgtcg ggttcgggca agaccacgct gctcaactgc ctgtccggcc tggagcggat
     1741 cgacggcggc tcggtgatca tcggtgggca gaacctggca tcgctcgacg acgatcgccg
     1801 caccgacctg cgcgcccgca cgatgggttt cgtctttcag gccttcaacc tgctgccggt
     1861 cctgtcggcc gccgagaacg tcgagctgcc gctgctgctg caaggggtga gcgcgcggga
     1921 ggcgcgagcg ctggccgtgg cgatgctgga gcaggtcggg ctcggcccgc gtgtgtcgca
     1981 ccgcccggag cagctgtcgg gcggcgagca gcagcgcgtc gcggtcgcgc gggcgttggt
     2041 ccactccccc gtcgtcgtgt gggccgacga gccgaccggc aatctggaca ccgagtcgac
     2101 cgaggcgatc atgtcactgc tggagcggct caacggtgaa gggcagacca tcgtgatggt
     2161 cacgcacaat gcgacgctgg cggcgcgcgc gaaccgcatc atccacatgc gcgacggcct
     2221 gatcgaaggc gtggagacgc cgtccgcgcg aagctgacat ggccgcagcc gcgatcccgc
     2281 tgctgctgtt cgcgatcgcg ttcggcctcg tggccgtggt cgggcggcgc aacccgatgc
     2341 tggcgcgcat ggcggcgcga gaggccacgc ggcggcgcgg ccagacggcg ctggtcgtcg
     2401 gcgggctgat ggtcggaacc gcgacgatca ccgccgcgct gatcgcagcc gactcggtcg
     2461 gcgactcgac caccgacgcg ttcgcgtacc ggaactgggg atacgtcgat ctgacggtga
     2521 tggccgacgg aaaccggttc ttcgacgcgg gcgtcgccgc ggccctgtcg gccgacgaaa
     2581 ccgtccgtag ggttaccgac gggatcgccg cgggcatcga ggtggtcggc tcggcgtcgg
     2641 atctgagcac gcggcaagga acgtcgggcg tgaacatcgt cgggttcgat cctgcggcgc
     2701 agaagccgtt cggctcattc gtgctgactg atggccggcg cacgaccggc gaagatatgc
     2761 ccggcggctg ggtgctgatc tcccggacgc tggccgacaa gctggatgcg aagcccgggg
     2821 atcgcttgcg ggtgcgcgcg gtcgcagcgg gcttgcaggg cgcggccgga acggaactgg
     2881 ccttgttcgg catcgcccga tcggagggac cgggcgccta cacgctcggc tcggccgtgt
     2941 tcgcgccgct gccgacggtg cagagcatct tgggcaccgc gcagatcaac gtggtgcggc
     3001 tgtcggcgcc gggcgggatc cgagacgccg acgatgcggc gcgcgccgcc gcggcgccgg
     3061 ttcgcgcggc cgttgagcgc gtcgccccga gcctggaggt cgagaccgcg aagctgcgcg
     3121 cggtggactt cgccgccgag aacaccatgt tcctgcgcgc gatgctcacc ggcatgagcg
     3181 cgatcgtggt cgcggcgggc gcggcgctgg tggtcaacat caccgggatg ctcgccgaag
     3241 aacgccgcgc gcggctgggc gtgatgcgtg cgctgggcct gcggcggcgc aaagtcgtcg
     3301 cgctgtcggt cattgaagga gcgatgtatg cgcttgccgc cggcatcgcc ggcgcgatcg
     3361 tcggggtcgc cgcgggccgg ctggtcgcgt cgcgtttcgg ccgcgccttc gccgagttcg
     3421 cgggcgagga cttcgacttc gagttcttct tctcgctgcg gcccgaaacc ctggcgaccg
     3481 gattcgcggt cggctcggtg ctgacgctga tcgtgatcgc gatcgcgtcg cggcgcaccg
     3541 ctcgcatgac catcaccgcg gcgatccgcg acctgcccga gccgccgccc gagaagccgc
     3601 gacaacagtg gccgcgcaac gcgcgcctgg ccgtcggggc gctcgccggg ctgtttctgg
     3661 tgtcgcaatc atcgatcttt ccgcggatgg cgggagccga gctgttgacc ctggtcgcat
     3721 cggcgttcgt ccggccgaag ctgaacccgc gcgcccacgg cacgctcacc gggcttgcga
     3781 tggccgcggt ggccttcggg ttgatcgccg gacaggcgcc cgagggcgat ccggccgcgt
     3841 ttttcggcgg cttcgtgatc gcgatgctct cggcggtgtt cggcttgacc attctggtca
     3901 cggccaacct gcacgtcgtc gagcgcggcg tgtcgctcgc cgggcgcgcg atgtcgcggc
     3961 tgattgccat cgtgcggcca cccatcgcgt atctgtcgcg ccggccgatg cgcaccggcc
     4021 tgacgaccgg cgtgttcgcg ctcatcgttg cgatgctgac gctgttcgcg gtgttcctgg
     4081 tcatcttcca gccgtcgctc gagcagttca gcgccggcta cgacgtccgg ctcctgtcca
     4141 ccggctcgcc ggatgtgacc atccccgatc aagtacgacc ggacgtggag cgcgtcgcga
     4201 ccatcccgac gcgcggctac gtgggaccca tcgaaggaag tggcccgttc tccagcgcgg
     4261 accgcgcgtt cgtgccgctg ctggtcgcgc cctccgacgc gccgaccaat ccgccggttc
     4321 gcctgttcga gcggtcgcgc cgctactcga gcgacgaagc cgcatggcag gcgatccttc
     4381 gcgatcccac gctggtcgtg tccaacttcg gtgagcccga cgccaagatc accttgcggg
     4441 gccagaacgg tcccgtgacg ttcaccgtcg tcggctcgca ccagttcgga ctgctggacg
     4501 gcgtcttcgc ctcgccggag gcactcaagc cgttcgagtc ggcgccgctc ggcgcgtcgc
     4561 tgctgatcga tctccgcgac ggcgcggacg cgcgcaccgc cgcgcgcacc atcgaacgcg
     4621 agctgttcgg cgtcggcgtc gatgccgaca gcacgcagga tctgtacgac accgcgtcgc
     4681 gcggcagccg agcgttcttc tcgaccatcg acgtgctgat gcggctcggc ctggtcgtgg
     4741 gcatcctgtc gctgggcatc gtcgcgatgc gtgtggtgat cgagcggcgg cagctgatcg
     4801 gcatgctgcg cgcgatcggc tacaagcgaa agcacgtcct ggccgggctg atggccgaag
     4861 cggccgcgac cgcaacgatc ggcgtcgccg tcggcgccac cgtcgggttg ctgatgggct
     4921 acatcttctc gacgcaacaa gaaggcgccg ccggattcgg cgtggattgg ggctcgttct
     4981 tcggcgtcct cggcgtcgtc tacgtggccg tcacgatcgt ggcgctcggg cccgcgtggc
     5041 gcgcctcgcg cctgcctccc gccgaagccg tccgctacag cgaatgacgg ctcactcggt
     5101 gccgctgcgg atgcggccga gcttcgcggc ttcttcgcgg cgcaggccgg cctcaacggt
     5161 gagctgcccg gcttcgagct ggtcgtcggc gatcaggcgc acgcgcagct ggccggggac
     5221 ggtgtagccc tgctcgcgcg cccaccaggc gagggtgtcc ttgagcgtgg cgaccagctc
     5281 gcgtttgcgg ccgtcgaagc gcgcgaggtc gtgctcggac agcgcgaccg tatagacggt
     5341 ctgcacgtag gtcgtgtcgc ggcgcacgaa gcggccggcc tccatcgtgc gtgcgagctc
     5401 ggtggcgatg tcctgcggcg ccggacgcgg ccccagggcc cagaattccg ggtcgtggag
     5461 catggagtcg ggcgcgtcca tcggtctact cgaaccgcac gaccttgaag ccttcgccgg
     5521 cggcgtagcc cgtcggcgcg ccgatctcgc cggcgcgctg ccgcacccac tgctcgatct
     5581 cgtccatgtt ctcgacctgc gaggcatgca gcgacagcgc ctcgatcttg acgtcgatgg
     5641 tcgaggagat gtcctcgacg tgatccggct cggcgcccca gttcatcagc agcagcattt
     5701 tcggcttgtg cggctccaag ccctcttgtt ccaccagctc ggggaacatc aggcgggtgg
     5761 acgcgtcggg gttgatcgag aacatcacca gatcgccggc ggcgatgtgg tccgggtggt
     5821 tggtgtagaa gtccgtgtag cgcagcgtcg ggtccaggcc gatgatcacg tcgggcttgt
     5881 accgacgcac ctgacgcgcg accgcgcggc gtgactcttc gttcacgacg aggtagccgt
     5941 cctcgaagcc gagaaactcg acatgggcga cgcccagcac cttcgcggcc gcctcttgct
     6001 cggcgcgacg aatctcgacg agcttctccc gagtcatcgc cggatcgctc gacccgctgc
     6061 cgccgttggt aatcatcacg taggtgacct cgacgccggc gcgcacccat ttggccacgg
     6121 tccccgacgc gccgaactcg gcgtcatccg gatgcgcgta gatgaccatc gcgcgcgaaa
     6181 tgtcgagcgt atcgtcgaac ctcatcgcga acctccccgt gggactctat cagccgtccc
     6241 gtccggccaa ccgccggatc aggctcgcgt attccgggcc gatgccgttg tcccgcagcc
     6301 cgcgaaggat gacgtcgaga taacggtcgg acggtggctc gggcgaggag aagttggcga
     6361 cgtacacgag cgcctcgaca ggctcgggcg cgtgcaccgt gatcgtgtgc cgccggtagg
     6421 cgaccggata ggtctcccac tcgtcgagcg ccgccagatg cgtatcgtcg atctcccaga
     6481 gcacgccgta gacctcggat gcgggatccg gcacgatggt cgcgacgcct ccgtcgtagc
     6541 cggtctcgtc ggtgacgaag ccgagacggt agccgggcag gcgggccggg ccgaccacgc
     6601 acgcaccggg acatcgtccg tccatctgga ccaggtccat gttggatccg tacgcgaagt
     6661 accgcacgac cggatctagg cttcttcgcc cttctccagc ggcggctcga agccgcactg
     6721 gtccatcaag gcgacgatgt cgtcctcgtc ccagccgcgc gacgcgagct ggtcgaccaa
     6781 ccggagcggt gcgagcgacg cctggcagaa cggacagtag gaccactcgg cccacgcctt
     6841 cgacggcaag cggccgcgac agtacgcgca gcgggtccgg tcaccgagcc acgcgctcgc
     6901 ctcggggatc ggcggctccg gcggcggctc catctcgtcg gcgtcggctt cggtgatcgc
     6961 atcgagatag ccgcgggcgt agtcccagcc cacgtactcg tccgggctcg gttcgaacgc
     7021 gggctcgtgc atgcgcggct cgcccgtctc gagcatgtgc tcgagcgact ccttcagcat
     7081 ctcccactcg tagtagtggt cctcgccgca ctcgctgcag acgatcacca cgcccttgta
     7141 gccgcgcggc tcgaagacgg cacgcatcgc ctcgaggtcc tcgagatcct cgaggatgga
     7201 ctctcgctct tccggatcga tatcgctcat gtggcgctca tgctaacgca gcgcagggct
     7261 ccgatcgttc ccgaggcaag ccccaggcga gaccctcgct tgccacggcc ggcgggttcg
     7321 gctgtaccat ttccgatcgt ggtgggcgat ccgtccccgc cggttgtccg gttcgacctt
     7381 ccgggcaggc agggacccgg ccccccaagg gcgaatggtt cgaatgctat gagtggagtc
     7441 cagggcctcg actcgggcgg gatcccccgc tggatcaccg gcctcgtctt cgcggccatc
     7501 gtcgccgcgc tggcggtcgt cgtcccggcc caggtccgcg cggcccacac acccagcccg
     7561 agcaacctgt gcgtgtgctt ccgaatgtcg cccaccgcgc cgaagctgac ctggagcgat
     7621 accgccgggt ccgccacgat ccagggctac atcctgcagc gttcgcgcac cgccgacttc
     7681 acctcggcgg tcatcgagat ccggattgcg cgcaacacct tcgacttcat cgacgagacg
     7741 atcgagttca acacgcgctt ctactaccgg gtcgcctcgt atctgggcag cgaccgcagc
     7801 ggctggagca acgtggtgtc ggcgaccaac ggcaacggca cctacccgac caagcccgca
     7861 acgccgagcg acctgaccct gagcaacgaa gggggcgggc ttcgcgtctc ctggtcgaac
     7921 cccgcgacga acgagaccgg cgtgtgggtg caacgctcgt ccgacaacgg cgcgacgtgg
     7981 gagtaccgcg cgtcgctcgc gatgaactcg acctcctatc acgacaacga cgtaccggcg
     8041 ggcgcgccga tgtatcgcgt cgcgacgttc aactcggccg gagcctccta ctcgtactcg
     8101 gttcagtacg tgaaccagaa cgcgcccggc gtcccgacgg tcggcccgat cgcgccgtcg
     8161 attcccgcga tgtcgattcc gggcttcccc tccgtgggca ttccgtcggt cggcttcccg
     8221 acggttcccg gcttctcggt tcccccgggg ctgatcccgt cgaacccgac ggcgatcgcg
     8281 agcccgtggc tgaacatgct cccgtcgaac ccgacggcga tcgcgagccc gtggctgaac
     8341 ctgatcccgt cgaacccgac ggcgatcgcg agcccgtggc tgaacatgat cccgtcgaac
     8401 ccggccacgc tggtgccgac cgtgcgcgat ccgctcacct atcgcgtctg gatcggcctt
     8461 cgcttcggca gtccccagaa ccagccggta ggcgtgtgga tcctgcccgc cacgtgcttc
     8521 ggcttcgggt gcccgccgcc gtccatgcag cctccatcga actgcatcgg attccggggc
     8581 gccctggagc tgtgctacct cgccgacgcg acgctgaact gatccgcggc tcgctcagga
     8641 acgtccggca gcaggcgcga acgtctccag gatcgcctcg aacgtctctt cggtgcgcgt
     8701 catgtccggc gacgcccacg gcgggccgag gatgagctcg tcggcgcggc cctcccagcg
     8761 cttgaccttg tcgcgcacct cgtcgggcgt gccgaagatc gcgtacacct cggccatctc
     8821 gtcggtgacg gtgtcgatca tgccttgcat gtcgcccttc gcgaacttct cgcgcagcgc
     8881 gacggtcatg tggccccagc cgtggatctc gaagatcttc gcataggtgc gggtcgtgcc
     8941 gtaaaagccg atctgaagct tcgcgcccgc gatcgcggct tcggtggtct cggcgatgct
     9001 ggtgatcgcg ccttgggcga cgcggcattc ggagggcttg cggccggcct ttgccgctcc
     9061 ggcgtcgatc gcgggccgca cgatctcgtc gaagtacttg acgctgctga acggatgacc
     9121 caggtacacg tcacagacct ctccggcgag cgagagcatg agcggattga cggcggcgac
     9181 ccccacgggg atcggcgacg gcgggggtga gccgcccgga aacggcggca tggtgaccgt
     9241 gtagaagcgg ccctcgtgcg agatcggctc gcccgcgtag gcgtcccaga ccttgcggca
     9301 cacctcgatc acctcgcgga tcttcggcgc cgggtgctcg aacggcgtcg agtagcgaag
     9361 ctcgttgacc cgcttgacct gcgtgccgag accgagaatg aaccggccgc cggagagctc
     9421 ggccaggtcg cgtgcggtca tggcggtgat caccggcgag cggggaaacg cgagcgcgat
     9481 cgaggtcccc acggtcgcgg tggtggtggc ctgcagcgca acggcggcct ggatgtaggc
     9541 ggtgcgcgcg gtctcggcga cccagatcga ttcgtagccg gccgcttcgg cgcgtctcgc
     9601 caccgggccg atcagctcca gcggcccacc cacggtcatc ccgtacttca tcgctcgcgt
     9661 cctttcgccg ggttccccgc aactctatcc ggcgacgcag ccgcgatacg atttcgccat
     9721 cgtgaccatt gcagagacct tcgcagagtt tgccgcggac atcacgctcg cgcgactgcc
     9781 ggacgacgtc gtcgccgccg tgcggcgacg caccctcgac ctgatcggct gcatgctcgc
     9841 gtcggccgcc gacggaagcg cggcgccggt cgtggacgcg gtgcacgcct gggcgggctc
     9901 gcgcgagacg ggcgtcgccg gctacgactt tcgtgcgccc gccccgcaag ccgctttcgc
     9961 caacggtacg ctcgccggcg cgctggtcca tgacgccgtg cacgtgccgt cgctcgcgcc
    10021 gctggacgcg gccgtgatcc cggccgcgct ggccgtcgcc gaggaagccg gacgcgacgg
    10081 cgcatcgctc ctggaggcca tcgcgatcgg atcggagatc gcggcgcgca tcgcggtggc
    10141 cgaagggacg gttgcgctgc cggccggcgc agcggcggct gccgcccggc tgtggggcct
    10201 gtccgaacgc gagacggccg cggcgatcgc gctcgcggcg acctcggcca cgcccgcgtc
    10261 cgagcatcgc gcgcggctgc gtcgcggctg ggcggccatg accggcgtga tcgccgccga
    10321 ccttgcgcgg cggggcgtcg ccgccgatgc cggcgtcccg gccctcgacg tgccgctccg
    10381 cgacgccggc gagcgctggg aaatgcgctc ggtcgcgatc aagcggtacc cggccggcct
    10441 gtggctgcac gcaccgatgg acgcggccgc gggcctcggc ttgaaggcgg gcgatatcga
    10501 cgaagtgctc tgcgcactcc cgccgggcgc agcggccgtg gtgtgcgagc cgcgcggcga
    10561 gaagatccgt ccggtcaccg acgacgaagc gcgcttctcg ctgccgttcg gggttgccac
    10621 ggcaatcgtc gggggccgct cgccgtcgga gatgttcagc gaggacgcgc gaacggaccg
    10681 ccgggttctc gcgctcgcgg cgcgcgtcgg ctacgaggag aacgccgcgc tcgaccccac
    10741 gggcgcgcgg gtccgcatca tgctccgcga cggccgcaca cgggacgcgg cccaacaggc
    10801 ctgcgacggc tcgccggccc gcgagctctc cgacgaggac cttcgcgcga agttcaccgc
    10861 gaacgcgcgt cgccgtctcg accagccggc gaccgcgaag ctcgcgacgc agatcatgcg
    10921 gctggaggac ctggacacga tcgacgaaat cgtcgccctc gcgcagggag acggctaagc
    10981 gaacgccgcg cggatctcgg tcgcgaagat ctcgagggat ccgctgtcgg gatagtcggg
    11041 gaaccaggga atcacggcct cgcatcccag atccgcgtag gccgcgatct tgtcgatgac
    11101 ctgttgcgtc gtgccgatca ggttcgtcgc tcgcctttgc tcctcgggac ggccgaagat
    11161 ggacggatgc cgctcggtga gcgcgccgag cgcgggctcg tccggcgcca cgagcatctc
    11221 gcagtgaatc gacagcttga tcgaggcggg gtcgcggccc atctcctcgg cgtagccgcg
    11281 catgatgtcg cgcttgcgct tgaaggtgtc cacgtcgccc atgaagttcg cccagtcggc
    11341 gtgcccggcg gtaatgcgca gcgtgatctg ctcgccgccg ccgccgatcc agacccgcgg
    11401 gcgcgggctc tgcaacggct tcgggtcgtt gaccgcgtcc ttgacgctca cgtacgttcc
    11461 gtcgtaggtc gcccgctcgt cccgccacat gcgctcgacg atgttgacgg tgtcccgcag
    11521 gtgaccgagc cgttcacgcg gctcgtggaa gccgtagccg taggcgacgt attcctcctc
    11581 gtaccagccg ccgcccagac cgaactcgag gcggccgccg ctggcgacgt ccacgcatgc
    11641 ggcgatcttg gccagatacc cgggattgcg atacggggtg cacgtcacca tctgtccgag
    11701 ccgcacccgc tcggttcgca tcgcgagcgc catcatgccg gtccagcatt cgaaaacggg
    11761 cacgcccggc tcggccggcc atgggacggc gtgaaaatgg tcgtagagcc agatcgagtc
    11821 gagcccgagc gactcgcagc gctgcgccac ctcgatcatc ttcgcgaacg cggcctgcgg
    11881 gtcaagcccg tcgagatcgt gcttccagcc ttgcggaacc agcgcgccca agtgcatcaa
    11941 cccatcgcct ccttatcgag cgacgatcat cgagcggccg ccgagcccga cgtcgggcaa
    12001 cggccagtcg ctgcgcacgt gcacgccgcg cgtctcgcgc cgctcgagcg cggccgcgac
    12061 gatcgtgacg cacagccgcg cgtgccacga gccgctgatc tcgtcgagtg cgaccaggcc
    12121 gtgcttgagg ccctcctcgt cgcgcaccgg gccgcatcgc tgccacatga tcgcgggcag
    12181 cgaggcgggc agatcgccga gcggctccgg tccgtcctca agacgtcccg acggcgcggg
    12241 caggccgccg agtccttgcc caacccgccg tccggtgacg acggcctcca gcagcgagtt
    12301 gccggccatg cggttggcgc cgtgcatgcc ggtcgtggcg cactcgccgg cggcgaacag
    12361 cccatcgacg ctggtcgcgc cgtccgcgcg cgccgcgacg ccgccgatga agtagtgcgc
    12421 ggccggatag accggcagcg gctgcgtgac cggatcgaac ccgtgccgcc gcgcgccttc
    12481 gacgatggtc gggaagcgat cctccagata cggcacgccg cgtgcgtcga gccacgcttc
    12541 gccggcgtcg atgatcgcct tggcgacgat gtgccgggcg gccatctcct cgacgaagcg
    12601 ctcgccgtcg gcgtcgatca aagtcgcgcc ttcgccgcgc agcgcctcgg tcagcagcac
    12661 gcgctgctgc gcgccgccca cggccagcgc ggtcggatgg aactgcatga actcgaggtc
    12721 gccgatcgcg gcgccggccg ccatcgcgag cgcggtcccg tcgccggtcg cgccgggtgc
    12781 gttggtggtg gacgcccaaa gcccgcccgc gccgccggtg gccagcagcg tggcgcggcc
    12841 caggaccgtt ccctggtcgg tcaccacgcc ggcgcagcgg ccgtcctcca tcgcgatcgc
    12901 gcgagcgcga gcgtccatgc gctgcgcacg gccgcgggct gcggcgcgaa gggcgcgaac
    12961 gatctcggcg ccggtcgcgt cgcgccagtg gacactgcgc ggcacgctct gccccccttc
    13021 gcgagcaaga tcgagaccgt cgccgtcgcc ccggtcgaag tcgcagccga gcgcctgcag
    13081 ccacgcgacc gccgcgggtc cctcaccgca gatgtcggcg accacgtcgg gatcgcacaa
    13141 gccggcgccg gccgccatgg tgtccttggc atgcagctcg gccgagtcgt ccggcccgac
    13201 cgccgcggcg agcccgccct gcgcgagcgg cgaggacccg gaggcggcct cgcccttgtc
    13261 gataaccgcg atcgaggcgc cctcgggtgc gctcagcgcg gcgcacaagc cggcgacacc
    13321 gccgccgacg atgaccaggt cgtagacatc cgccatgagt ggcgaatcgt agtcgaagcc
    13381 cgcgcgggga tgccgcctgc tacaggcgcc agtgcggctt gagccaggtg cgacggccac
    13441 atcccgggca cttcaagaac gcgggatacg gacgcgggac cgccatccag aacggcatcg
    13501 ccagccgcgg aagctcgcgc agccgcaccg gcgactcgcg cttgcacgag gagcattcga
    13561 gtgtgagcgt gcccatcggg ccggtcccgc gggccccgac gctgtagagc gcgcgcttgc
    13621 ccatcggatc gggctcgcgc tggcgacgcg agtagtcctg gagctgctcc tcgatcagga
    13681 gcctgtccca gtccacgggc cggtcgtgct cgctcacgat gccgcgtcct gctgcggaag
    13741 gccggtgagc ttgcgcgcga cctcgcggta ggcgcgcgtg cccggatgcg acggcgcgaa
    13801 cttcagcatc gagcggcccg tacgcggcgc ctcggcgaaa cggatcgact tgcggatcgc
    13861 cggctccagc acaggaaggc gatagcgccg ggccacatca tcgagcacgt ccttggaatg
    13921 cgcggtgcgc gcgtcgaaca tggtcggcag gatgccggtg acctcgaggt ttcggttggt
    13981 cagccgcttg atgtcgtcga tggtggccag cagctggccg acgccgcggt gcgacagcgt
    14041 ctcgcactgc atcgggatga tgacctcgcc ggcggccgtg agcgcgttga tcgtgagcac
    14101 gcccagcgac ggcgggcagt ccacgacgat gaagtcgtag tggccgcgca cgtcctcgag
    14161 cgcggcgttg agcgtgtatt cgcgtcccgt acgggtcagc aggtaggcct cggcgccggc
    14221 gagatcgatg ttggccggaa cgagatcgac ctcgtcgagc tgcgtgatca ccttgccgat
    14281 cgggacgcgc cccaccagca cgtcgtggat gctcgtgtcg atcgtgtccg ggtcgaggcc
    14341 gagcgagaag gtcaggcacg cctgcgggtc caagtcgagg gcgagcacgc gcggcccctg
    14401 ctcggcgatg gccgctgaca gggcgatgac cgacgtggtc ttggccacgc ctcctttttg
    14461 attcgcgacc gcgatgacgc gcgccatcgg cctctcccct ctccggcttc gctcagccgg
    14521 tccaatgttc ggcggaacac gacgggaatc gcaaagccgt gaaaatcccg cctgccgcct
    14581 caaggccgag ctcgccggcc ccccggcttg ctcgcaccac tggatacact acgccacgga
    14641 tgcccgaatt cgacccaacc atcctgccgg gggcctcgtc cgaggccccg cacggcggcc
    14701 gtttggcggc cgcggcgctg cgcgagggcg gcgtggacac catcttcgcg ctctgcggag
    14761 gccacatatt ggggctgctg gacggatgcg tggacgaagg cattcgcgtg atcgacgtcc
    14821 ggcacgaagg cgccgcatcg ctggccgccg agggctgggc gctggccacc ggccgcaccg
    14881 gcgtcgcggc cgtgaccgcg ggaccgggct tcggcaacgc gctgacgggc tttatcgacg
    14941 cgggcgtgtg gacgctccca atggtgctgc tggccgggcg caccggcgtg gcgcagcagg
    15001 gccgcggcgc ggtgatggac gtcgatcaac gcggcatcgt cgcacccgtc gcgaagtggt
    15061 ccgcgacgtg ccttgcgaca ccgcgcatcc cggcagtgat cgccgaggcg ctgcaccgcg
    15121 cgcgcgccgg caagccgggc ccggtctacg tcgagatccc gcaggacatc ttcatggcct
    15181 cggccgagcc ggattcgccg cgcgcggcgg cgggcgtccc gtcggttgcg cccgcgccgg
    15241 cgggcgatcc ggccgagatc gcacgcgcgc tggacgtgct gcagcgggcc gagcggccgc
    15301 tgctgatcgc gggcggcggc gcgttctggt cggacgcggg cgacgcgctg gcccgcttcg
    15361 cggaaacggc ttcgatcccc gtggccacca cgagcgcggc gcgcggcctg ctgccggact
    15421 cgcacgaatg gtgtctgggc tccctggtcc atgcgggcgt ggcggcgctg gccgccgacg
    15481 tggtcgtggt cgccggctcg gcgttcaacg cgaacatggc gttcgggcag ccgccgctca
    15541 tcggcccggc cgtgacggtc gtgcagatcg acatccggcc cgaagcgatc ggcggcaacc
    15601 gcgccgccga catcggcatc gccggcgacg cggcgcgcgt gctgggcggc ctcgccgacg
    15661 gatggaccaa gacgcccgac ggccgcgacg cgtggctggc gcaggcgcgc gagctcgccg
    15721 ggcacggcct ggcggcttgg gatgcccaga tcgacggcta taccgggccg ggcattcact
    15781 gcggcgcggt cgcgcgcacg acggcgcgcg tgctgcgcga gacgtgcggc ggcgacgtga
    15841 ccttcgtcgc cgacggcggc gacgcgctgt cgtgggggct cgcctacggc tacgcggagg
    15901 gccccggccg aatgatgacc accaccaccg cgctgggcac gctcggcgtg ggcatgccgt
    15961 tcgcgatcgg cgcggccgcc gcacggcccg gcgagccggt ggtgctgctg accggcgacg
    16021 ggtcgttcgg cttgaccgcg atggaggtcg acaccgccgc gcggcacggc ctgcccgtga
    16081 tcgcgatcgt gtccaacaac ggaagctggg gcgacgtggt gcacgagcag cgcgcctggt
    16141 acggccgcga aaccgccagc gcgctgaggg gcgcccgcta cgacgtgctc ggagaggcgc
    16201 tgggcgcgcg cggctggctg gtcgaatcct tcgaggcgtt cgagcccgcg ttgcgtgagg
    16261 cgctggcaag cggcggcccg tgcgtcatcg acgtgcgcac cgaccccgag atcctatcgg
    16321 acttgcttcg caacctttca tcgatgaact tgatgtagct ccgccgagcc gaagggaccg
    16381 caatgagcga cctcatcacc tacgacatca acggcgccgt cacgaccatc acgatggacg
    16441 acggcaaggt caacgcgctc tcgcccgcga tgctcgccgg gatcaacgaa gcattggatg
    16501 ccgccgaaaa ggaacgagcg acggtcgtgc tggccggacg cgacggacgc ttctcgggcg
    16561 ggttcgatct gacgatcctg cgtgcgggcg gcgacgaggc gatcggcatg ctgcgtggcg
    16621 gcttcgagct gtccgagcgg ctcctgtcgt tcccgacgcc ggtcgtcatc gcatgcaccg
    16681 gacacgcgat cgcgatggga tcgttccttc tggcgtccgc ggactatcgg atcggcgccg
    16741 ccggcgactt caagatcgcc gccaacgagg tcgcgatcgg cctgacgctg ccgctcgctg
    16801 cgatcgagat cctgcgccag cggctcacgc ccgcggcatt ctcacgcgcg acgctgctcg
    16861 cggaaacctt cacacccgac gacgccgtcg ccgccggatt cctggaccac gttgcgccgc
    16921 ccgccgacgt cctcgccatc gcgcagacgg tcgccgccgg catggcgggc ggcctccaca
    16981 tggatgcgca cgcggccagc aagctgcgcg tccgggcggc ggcgctcgaa gcgatccgcg
    17041 ccggcatcga cgcagagttc cccgacggcg agggaccagc cacggcaatc attcagcggg
    17101 gcaggtcggg ctagctagat cgggcccggc tcggggtggg cggcgtgatg ctcggcgcgg
    17161 atgcgagcga gcagccacac caggccggcg aagccgacga tcgatccgcc gaggaacgat
    17221 ccgcggtagc cgtacaggtc ggcgaggaac cccatcagca acggcccggc gaggaagccg
    17281 aggtccacga acgcggtgaa ggtgccgacg acgctgccgc gctcgcgggc cggcgcgccg
    17341 cgcacggcca gggtcaacat ggacgggaat acgaacgcct ggccggtcgc gaacagcatc
    17401 gatcccagca cgagcccgag gctcccccgc caggcgaaca ccaggagcaa gccgctggcc
    17461 gagaccatta acgcggtcgt ggccgtgcgg cgcgcaccga accggtcggg gacgcgcgcg
    17521 cccgccaggc ggagcacgag gatgacgacc gaatacgcga ggaagatcgg ggcggcgtcc
    17581 atgccgaggt tccgcgcgta cagcggcatc aaggccgcgt agccggcgaa gccgatcacg
    17641 ctcgtggtca tgatcgaccc gggcccgaac gcggcgcgat ggaagatcgg cccacggcct
    17701 tgcggcccat ccttgtcggg ccgggtgtcc ggaaggcgaa gcgtggcgag cgccgcgaca
    17761 cccgcgagcg cgccggcggc gaggaatgcg gccttgaacc ccgcggcctt catgatcgct
    17821 tcgccggcga gcgggccgag cgcgagcccg gagtagagcg agagcgtgaa gtaggacacc
    17881 gcttcgccgc gccgctcctc gggcgcgagg tcgttgatcg cggaggcggc cccggtgaag
    17941 aaacccgcct cgccaaaacc tgccgagaac cggaacagga acgcgagcca cacggcccct
    18001 gcgtagccgg tcggcagcgt cgccgtcgcg acgatcgcgg caccgccggc catcaggatc
    18061 ccgcggccgc gtgcgtcgcc gacccgtccc gcgatcgggc gcagcacgat cgaggagaac
    18121 gcaaacgccg aaccgaccag gccgacctcg aggccgccgc cgcccaacgg cccgtccacg
    18181 aacttgggca gcaccggcag cgtcatgccg taggacgcga aataggcgaa cgaagtcagc
    18241 gtgatgcgaa cgaagagcgg cgtgaccagt cgcggcgccg cagcgccgcc ccggccgctc
    18301 atgcgcgcag catcgcaagc cgctccgccg cggcctgcag cgtctcgaga cgtttgcaga
    18361 aggcgaagcg cagcttggtc cggcccagcg acggatcggc gtagaaggac gagccgggga
    18421 cggtcgcgat gccgggatcc gctgcgaggc gcttggcgat ctcgacgtcg tcgccgtccc
    18481 aaccgaacgc cgagatgtcg gtcatgatgt agtacgcgcc ggagggcttg tagaccttga
    18541 agcccgcatc ttcgaggatg ccggtcatca ggtcgcggcg ctccaaatac tcctcgcgca
    18601 gcgacttgta gtaggcgtcg ggaaagttga gcgcgaccac gccggcctct tgcagcggcg
    18661 cggcggcacc gacggtcaga aagtcgtgca ccttgcggat gccgtcggtc agctccggcg
    18721 aggagatggt ccagccgatc cgccatccgg tgacgctgta ggtcttgctc atcgcgttga
    18781 tcgtgacgct gcggtcttcc aggcccggca cggtcatcgg cgggatgtgc tcgcggccgt
    18841 cgtacaccat gtgctcatag atctcgtcgg tgaagcagat cacgtcccac ttctggcaca
    18901 ggctcgcgat gaactgaagc tcctcgcggc tgaagacctt gccggtcggg ttgttgggcg
    18961 tgttgacgat gatcgcgcgc gtgcgctcgt tgaacgcggc cgccagctcg gcctcgtcga
    19021 aggtccagtc gggctcgtgc agcgtcacgt agcgcgggac cgcgcccgac aggatcgcgt
    19081 cgggcccgta gttctcgtag aagggctcga acacgatgac ctcgtcgccc gggtccacga
    19141 tcgcgagcgc ggccgcgatc atcgcctcgg tggagccgca ggtgacgcag atctgacggt
    19201 tgggatcgac cgggacgccg gagaagcgct cgaagttcgc cgcgatcgcc tcacggaagc
    19261 gctgggcgcc ccaggtgatc gcgtactggt tgacgtcggc gtcgatcgcg gccttggccg
    19321 cctccttgag ctcgggcggg caggagaagt cgggaaagcc ctgcgcaaga ttgatcgcgc
    19381 cgtgctgcat cgccacacgc gtcatctccc ggatcaccga ctcggtgaac gaggcggcct
    19441 tcgccgagat tcggatctgc cccacggtgc tcgtctcctt ccgccgcctt gtggtccttc
    19501 ggcacgatat cggccccgac gccgtcgcgc accccgatgg ggatacgtcc ctggttcggg
    19561 accgtgcgcc ctcgaactcg ggaccttcgg cctatgagcc cgccaccgcg ccgcccgata
    19621 ttcatggcat gcactcccct ggaggcccat caccgtggca gggactcctg catcggcgcc
    19681 ccgagacagg cggcgtcgcc atcatgatgg cgatcatgat gctggtgtcg ctggtcatgg
    19741 cggcgttcgc gatcgatctg agcaacgcgc gctcggaccg gacgctggcc gactccgccg
    19801 ccgattccgg agcactcgcc ggtgcgcagt cgcttcccga tcccgcctta gcgcggctcg
    19861 acgcgcgcga gtacgcaacg gccgcgctca ccggaggcga gacgggcacc ggcgcgacgg
    19921 tcacggtgac gacgccctac acgatgagcg gcaacgcgta tcccgcgcat cggctcgtca
    19981 acgtgcgcgt ctgctggaac agcccgaccg ggttcgccgc ggcgttcggc atcccgtccg
    20041 tcaacgtctg cggagtcgcg accgccttca agacgaacgg gaccccatgc ggcctgtgcg
    20101 tgctcggcac gaccgggcgg accttgtcga tcgccggaaa cggcaccctt cgggtcacgg
    20161 gggccgacgt gcacgtgaac tcgaacggca caccggccgc cggggtcgag ggcaccgcga
    20221 ccggtggcgt gacaaccgac ggagtcattc ggattctcgg cacctaccag caaccgagcc
    20281 cggggacctt cagtccgacg ccgatcacgg gcgtgcccgc gattcccgac cccctttcga
    20341 cgattcctgt cccctcggtc gccggtccgt atcgcgggtc ggtcgcgcac acgggcccgg
    20401 aaaatctgac gctgcagccc ggcatctacg actcgatctt gtccgagtct cgcggcgtgc
    20461 tgaccttcag ccccggcatc tacgtgatcc gaaccgggct gacgctgcaa aagagtgcgc
    20521 aggccccggt gccctcggtg gtgggcgacg gcgtgctgct gtacttcgga tgctcgctgt
    20581 acccgacccc gtgcctgtcc ggcggacaga ccggcgccgg gatctcgatg tcgggctcga
    20641 cgacgttccg gatcagcggc atgacctcgg ggccgtatac gaacctgccg atcttcttcg
    20701 accggaacaa caccgcctcg atgaccatcc tcggcggagt gctgaaccgc atcgacgggg
    20761 tcgtctacgc ccgctcggcc cactactcca tggccggaag ctcgggctcg atctcgcaca
    20821 acggcgcggt cgtcgtcgga cgcacgacca cgacggggtc atcgaacttc actgtcgcat
    20881 tcacgaccga ctacgctcat ccggtcctgg gccgccacac gttcctggtc gaataagcaa
    20941 gcttgcggac gccgctttcc ggcacgcgcc cgaactcgta caattcggtc agccggcagg
    21001 gagccggcgg tgtcagccgg caggcggccg gcccgatccc gggggatgca cgtgaccgac
    21061 atcgtccgct tcacgaacaa ctacagcgat cattcgaccg aagccggcta tcagttcgag
    21121 ttctattgca tgcgctgcgg caacgggtac aagtccggat tcaacgcctc ggcgctgggc
    21181 atcggaaaca aggtcgtccg cggcctgggc ggcctgctcg gcgggcggct cggcggcgcg
    21241 agctacgccg gccacgagct caaagacctg accgagtcgc aggccaagga caaggcgctg
    21301 cgcaaggcgg tcgaggaggt ctcgccgctg ttcaaccagt gccaccgctg cggctcgtgg
    21361 gtctgcaagg acatctgctg gaacgacgag gcccagctgt gcgtgaactg cgcgcccaag
    21421 ctcgagcagg agatcgccgg catgcagcag cagcgtcgct tgcagcaggt gagcgagaag
    21481 atcaacgagt ccgactacgc ctccgacgtc aacgtcgaac gaatgcaggt cgcgctctgc
    21541 ccgcactgcg gagccgaggc gcaacacggc gcgaagttct gcggcgaatg cggccagacg
    21601 ctcgcggttc aggtccagtg cggacgctgc ggcacgaccg ccgcgccggg cacgaagttc
    21661 tgtcccgagt gcgggaacgg gctctagtgg ccgcggttcc cgggcaggtc gccgtcgggg
    21721 gcggcgaccc ggtcgcgtgc acggtggaag cggccgagga cggcgttcgc gtcggcggcg
    21781 gcgttcaggc attcatcgcc tacgacgaca tcgacgacct gctcgacgac agctacacgc
    21841 tgcgaatcgc ggtcgaggac gggtcgcgct acgacttcgg catgctcgga cgcgcgtacg
    21901 gccagatcgc ggccgacatc gtcaagcgcc gcgacgcgtc gctcgagaag aacctgctgc
    21961 tgcgcggcgt gaacctgcgt gacacctatc cgggcatgct tctgggcgag gacgcgctgc
    22021 agcccgtcgg cgcgacattc cggctctacg aggatctgct cgtggtgcgg ccggatcacg
    22081 gcgtgatgtt cggagtgccc ttcagcgcgg tggacgcggt ggactgggac gccgagctct
    22141 accaggccca catccggctc gacgacggca ccaccgtcac gatcggcaag ctcgccaagc
    22201 gatccgagga gttcaccggc gagctgcgcc gcctgctcga cgcgctcgcc gagcgcaccc
    22261 gcgcgacgct gtccggcctg ctgccggacg tgtcgcccgg gacgatcacc gcgctggccg
    22321 gcgcgatgcg cgacggccgc gccgtgcaga agcgtaccgt cgacgcgatc gaccccggct
    22381 tgtggcccaa gctcgcgacg gccgtgctgg ggtcgcggcg cgactcctac gagcgactcg
    22441 ccgcgatgac gcccgacggc tgggccgcgt tcggcgtcaa ggcgctcaag gcggctgcga
    22501 gcgacgaggc ggccgaagag caaccaccgg ccgccgagcc cgaggatccg gagaccggac
    22561 ccgaagcgga gcggaccgtc ccgggcggcg gggagaaccg gctgtggttc ttctgcccgc
    22621 tctcgcggga cggcgtgccg atcaacgcgg tcgcgagcga ggtcacctcg gagtccggcc
    22681 acgcgaccta cgtgttccgg ctgatggagc cggaacggtt cgcgtcgctg cgtggcgacg
    22741 agctcgccga cgagatcgga cgcgcgatcg gacggctcaa tcgcgcgctg ctcctgctga
    22801 acttccgccg cgagccgctc tacgcctccg aggacgagat ccggtcgggg cggctcacgc
    22861 gctaccgcgt cgcgctgcgg ctgctcccgt acctgcgatg ggctcgcgaa tcgttcctcg
    22921 gccgcgccgc ccacacggcc ggctgggacg ccgcgatcga ccgaatcctg gaaaaggcct
    22981 gaccggacgg ccgaatcggg accgccaccg gcccgactcg ggactttcgg cctatcgacc
    23041 ggcctgcccg cgcggccgat accttcgcta tgaggggcat acagccgtcc gccgcgcgcg
    23101 gccagcgcgg cgcgagcgcg gtcgagttcg cgatcgtgct tccggtgatc gcgctgatgc
    23161 tgttcggcat catcgacttc ggcgtggtgc tcaacaacca gaacaccgtc agggccgccg
    23221 cgcgtgaagg agcccggctc ggcgcggtcg cccagttcac cggcggggcg ggatgcacga
    23281 tcgtgggcac ggtctcgagc accgagacgc gcgggctgat gtgcttgatc aaggagcgca
    23341 accatccgat cggcgaggcc gctcgggtgc gggtgatcgt gccgtctccg taccgggcgg
    23401 gcgagccgct cgtggtctgc gtgcagacga agatgtcctc gctcacggga ttcacctcac
    23461 cgttcttcga gagcaagacg gtgacggcca agacgacgat gcgggtcgag cgcacctcga
    23521 gcggcgactt cgcctcgggt caggaaacgg cgccgcccgg cggaagctgg acctggtgca
    23581 cctgagcggg cgttagcccg acagcgtgcg ccggtagacc acgaacccga gcgcacccag
    23641 cacgagcacg ccgaggatgg cgatgatcgc ctgcgagttg cccgacgacg gcgagccggt
    23701 cgtgtcatcg tcgaacgacg ggatcggcga ggccgagtcg gtcggctcgg tcggcgtggg
    23761 cgtgggcgtc ggggtcgtgg gcgtgggcgt cggtgtagag ggcgacggcg acgggctcgg
    23821 cgaagaagtg gttttcgtcg gggtcggcga cgaggtgcga cggcgcgtgg gggtcgaggt
    23881 cggcgtcgtg gtgatcgtga tcgtgcccgt cgcggtcggc gagggtgacg gcgagctcgg
    23941 gttgttggtg accttgacgg tcatctcgta accctccagg ctccggccgt ccagccggta
    24001 tttgtagttg ccgagcgtgg acaacgtcag cgggttggtg ctctccttgg gcgcgagctg
    24061 acggggcgag ggctgcggcg cgccgctctg ggcgtcgatc gtgtgggtga tctgaccgtc
    24121 atttttccag atgacgctgt cccccgggcg gatctcgatc gaggtttgga agaagccgtc
    24181 gtcgcgcgcg agcacggtgt aggtggcggc gtgggcctcg cgcggcacga agaccacggg
    24241 cagcgcgagc atcgccgaca tcaggatgcc ggcaccgagc cgaagtgtgg gtcgcttcat
    24301 tgatctccaa cccccgtcga acgttgccgc gtactttacc gcagttcttc ggccgggccc
    24361 gcgatccccg ggacgaaccg ggggacgcgc tcgatccggg gaccgggggt tccgtgcggg
    24421 gcccgcgacc ccgcacgcgc cgaggccggg cgccggggcc cggcctcgat gcggcaagca
    24481 aacggtgcga tcaggcgtac atcgactcga tcaggtccgt gtacttgtcg ctgatcgcgc
    24541 ggcgccggac cttcatggtc ggcgtcatct cgtcggcttc ctgcaggaag tcgcgttcga
    24601 gcaccgtcca cttcttgatc tgctcgaccc gcgaaagctg ctcgttgacc tcctgcacgc
    24661 tggtctcgac gagctggagg atgtcggggt ggcgcgcgag ctcggcgaag tcgccgtgcg
    24721 ggatcgactt ctgctccgcg aacgcggtca ccttctcctg gtcgagcgtc aggatcgccg
    24781 tcaggtacgg acgggcgtcg ccgatcacga cggcctgaga aatccacggc gagtgcttga
    24841 gcatgttctc gagcacctgc ggcgcgatgt tcttgccggc cgcggtgatg atcagatcct
    24901 tcttgcggtc ggtgatccgc aggtagcctt cctcgtcgag ctcgccgaca tcgcccgagt
    24961 gcatgtagcc gtcctcgtcg atcagctcgg tggtcgcctc cgggttcttg tagtagccgg
    25021 gaccgacgtt gccgcccttg accaggatct cgccgtcctc ggcgatcttg atctcgaccc
    25081 cgtcgatcgc cgggccgacc gtgccgatct tgatcgcgtc cggacggttg atcgaggtcg
    25141 ggccgcagtc ctcggtctgg ccgtagacct cggcgaccgg caggccgatc gcgtggaaga
    25201 accgcagcac ctcggggttg atcggggccg cgcccgagat catcgcgcgc gcttggtcta
    25261 ggcccagcgc cgcccgcgcg atcgcgaaca gctgcgcgtc ggcctgctgg taggcggcct
    25321 cgagctcgtc gggaagcgac gagccggcct gcgtcgcctc gacgcgcttc agacccagct
    25381 gcacggcctg ctcggccttg acgcgctgct cctccggcag gttgtccaga aggttcttga
    25441 tgccggcgta gaacttctcc cacacgcgcg gcacgccgaa gaagatcgtc ggccggcatg
    25501 cctgcagatc ctcgcgcagc gtgtcgagcg actcggcgaa ccaggtctcg gatccgcgcc
    25561 agatctgcat catgtggctc gtgacgcgct cggcgatgtg cgacagcggc aggtaggaca
    25621 ggctccggtc gtcgggctgg ccctcgagcg ccttgttcag cgactcgcac gtccacacga
    25681 tgttgtcgtg cgtgagcatc gcgcccttcg gcgggccggt cgtgccggag gtgtagacga
    25741 gcgtggcgag ctgctcgggc ttgatcgcct tgacctgctc ctcgaagcgg ccggcgttct
    25801 gcttgccgaa ctcgtcgccg gcggcgagca gatcggccca cgacatcacg aagccgtcga
    25861 gcgtcgcgcc gtctgtgtcg atgacgacga ccttcttcag ctcgggcagc tcgttgcgcg
    25921 tcttgagaat tttctcgacc tggccggcgt tctcgcagac gatcaccttc gcctcggagt
    25981 gacccacgat gtaggagacc tgcggcggcg agttcgtcac gtagatcgga acggtgacgc
    26041 cccccagcgc catcgtcgcg acatcggcca tgtgccactc gggacggttc agggacagga
    26101 tcgcgacctt gtcttcggcg cccagaccca gcgacagcag cccgttggcg gccttctgca
    26161 cgtcgctcgc gtactcgatc cagctgatcg actgccattc gccgccgcgc ttcaccttga
    26221 aggcgaccgc atcgcctcgt tcggcaacgg tcttgagaaa tagcgatacg acggtttcgg
    26281 tcattgcgcg ctccctcccg gctgacgcgg tggtggtttg tgtgccctca ttcctaccgg
    26341 gttgagccgc tccggcgcaa cggccagcgc cctagacgga ttcttcgagc atgcgctcgt
    26401 atcgctccgc cacccggtcc cagtgatggt tcgcctcgac gaacgcgcgc ccctgctcgc
    26461 ccatcgcggc ggcgcgcgcc gggtcggcga gcagaccggc cacggcttcc gcgagcgccg
    26521 ccggatcgcc gccgggaacc accaggccgg tctcaccggg gatcacggcc tcgggcgcgc
    26581 cgcccgagtc gccggcgatc gcgagccggc ccgtcgcctg cgcttcgagg tagaccaagc
    26641 ccagcccctc gacctcgagg tccgcgaagc ggctgcggca cggcatcgcg aacacgtccc
    26701 cggccgcgta atgggcggcc agctcggcga acggcgtctc gccggtgaag atgacgcggt
    26761 cggcgacgcg gagcgtgaag gcgagctttt cgagcgcggc gcgatgcggc ccgccaccca
    26821 cgatcagcgc ggcggcgtcc ggaacgcgcc gggcgatcgc cggcatcgcg cggatgatcg
    26881 tatcctggcc tttgcgcggc accagacggc tgacgcaagc gagtaccggc cgtccgtcga
    26941 ggccgtggcg cttgcggatc tcggacccat ccacgcccgg atggaacatc tccaggtcca
    27001 cgccggtctt gagcatctcg atgcgcggac cggcgccgac gaccggctcg atgaagcggc
    27061 ggcagtattc gctgacgacg gtcacgacac gcgcgtcggc gcagacgcgg cggagcgcgg
    27121 cgcgcgctcc cggaagacgc gtgagcgcga cctcgaatcc gtgcgtgtgg atcacgtagg
    27181 ggcggcccag cttcggcgcc atccagttca tcggcaccac cgcgccgaac gcgaccacgt
    27241 cgtggcggcc ggccagatcg gcgacgcggc gctgcagccc ggtgcgcggc caatagacgc
    27301 gcgggccgtg acgcacgatc tcgtacggct gctgcgcgtc ccacgcggcg gcgcgcggat
    27361 gcggcggcgc gaccacggtg atctcgtggc gcgcggcgag ccgccggtag atgttctcga
    27421 ggtagacctg gatgccgccg acgcgcggcg gatagtcgtt ggtgacgagc aaaatcctca
    27481 cggcgcggcc tccagtgcga tctcgattcg ctccatcacc ggcggatggg tgaagaacat
    27541 ccaacggatg agcgggtggg gccgcaagtc ggcgatgttt tcccgggcga ggctcacctc
    27601 ggtccggatc gccgtctgga cgtcgccggt gagtctgacc gcgaaccggt ccgcgtcggc
    27661 ctcgatggag cgcgagaagc cgttggcaag cgggagcgtg acgagggtcg cgacggccat
    27721 tgtgaacgcg agcagcggga tcaggcgcgg atcgccgggc ccggacgcgc cggcgaagcg
    27781 cagcacaccc ggccgtccgg tcaggagcca cacgagcacc accgccccga ttgcgccggc
    27841 gctacccagg atggttcccc tcagcacgtc gcgatgcacg acgtgcgcga gctcgtgcgc
    27901 gacgatcaga tccacctcgt caggcgggct gcgctcgagc agcgtgtcgt agagcacgac
    27961 ccgcttggtc gcacccaggc ccgacacgta ggcgttgagc cgttttgttc gccgcgacgc
    28021 gtccgcgacc aggacgcgat ccacggtcac gccggcacgg tcggcgagcg ccacgatccg
    28081 ctcgcgcgtc gccggatcga cgggcgtgaa cttgttgaac agcggctcgt agacgagcgg
    28141 gtacaccacg accatcacga cggtcagcgc caccatcacg gccgcggcgg cgagcggcca
    28201 cgtcaccggc agtcgccgga cgatgaagaa gaatgcgagg gcgaccacac ccgcgacaac
    28261 gacttgaacg cccgctccct tgcccacgtc ggccaggaag ccgccgaggc tttgcgtgga
    28321 cagaccgaag cgccgctcgt gccagtagcc gcgcgcgacc gccagcggca gggtggccag
    28381 cacggggatg agcgtgacga ccgcagcgag gatgagcgcg gtgagcggcc agcggccgcc
    28441 ggagacgcgc tcggcccacc cgccgagcgc gcgagcggcc ggaccgagcc cgacgatgcc
    28501 ggcgagaatg agcccgagca cgagcgaaga gaacccgagc acgtagcgcg tgccggtgta
    28561 gtcgcgggat cgctcgatct gctcttgggt gaagtagtcg gtgacgcggg gctcgtccgg
    28621 ttgcgcgccg atcgcgggcg cggccaaggc aaagccggcg gccgcgagcg cggcggcgat
    28681 caggatgagg cggaggcgcg agcggggggc ggtcacgccc cggatgctac cgtcgcgtac
    28741 ggcgccgcgc agcggtgcgc ttcttggcgg gtgcccgggt ggagcgccgt ttggcggccg
    28801 tgcgcttctt ggcgggacgc cgcgccgggg ccggacgctt cgccgccgtg cgcttcttgg
    28861 ccggacgctt cgccgcgggt cgccggaccg cggtccgctt cttcgcggcc gtgcgcttct
    28921 tcgcggccgt gcgcttcgcc gccgtgcgct tcttcgccgc cgtgcgcttc ttcgcgggtg
    28981 tgcgcttctt ggcaggtgct tttcttgccg aggccttctt cgcggacgtt ctcttcgcgg
    29041 aggccttctt cgccggacgc ttgcgggccg gcttctcggg tgcttcggcg gccgcgcggc
    29101 ggccgggcgg ctcgggggac acgaagcggg aggtgcgttc ccccgtccgc agacgtttgc
    29161 cgggcgcgac ccatcctaaa tcggcgccga gacggttcgc ggcggtcacg ctgcgacgcg
    29221 tcgggtcgta tttccacatc gcgcgctgct cgcgtccgcg gaaccggaag cgaagcgtga
    29281 tcgtccacgg ctggccctcg acgcgggtgg cgtcccacgc gttcgtcatc tcctcgtcgg
    29341 tcagccggac gtggcgcgag cgaaggttcg cctcgaccgc ctcgccgagc ggcagcccgc
    29401 tttcgccgag ccgctgcttg ctgagccacg cgcgctgcgc ctcgcgcacc atgccttgcc
    29461 gctcgtagag caccggcacc aggaactgct cgacccacgc gatcggcacg tcggcggcct
    29521 tggcgacctg ctcgggcgtg cggcccgctc gaagctgtcg ctgaacctca cgcggcggaa
    29581 gctttgactc gacgcgcggg gcgaccggac ggggcgtgtg gtcctcttcg tcgtcgatgc
    29641 cttcgccacg acggcggcgt cgcaccacgt cctcgagcac gtcgaacaga tcgtcgtcga
    29701 tcgcgacgag gtggctgcct ttcttgcccg tccgcttgtt ggagtagacg agatgcttca
    29761 ggtcggcgct cgacccgatc agacggagct tgatc
//
LOCUS       DATLFO010000121         8521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_44855, whole genome shotgun sequence.
ACCESSION   DATLFO010000121 DATLFO010000000
VERSION     DATLFO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44855"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VM841_04490"
     CDS             <1..413
                     /locus_tag="VM841_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89477.1"
                     /translation="SGIGFGAGTLAIAARDVPTAEGAYGTILIDTVGMVHDKPYRNVI
                     VGALACVVVEPVAATGGHRISTYSFAVDTFGVWPLFVTLDVGPDGSASFSAIRGYTTP
                     DGPCRGPEWTNVPLIGSTVLVRAGHFPSTGIGRG"
     gene            555..3968
                     /locus_tag="VM841_04495"
     CDS             555..3968
                     /locus_tag="VM841_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89478.1"
                     /translation="MKTHLAVRRLALPALAALLVSTLVSWPGARTDTQALACPPGYHL
                     EGEEEARLSLYGDVLDVRTGTELCIHDKHPETERELRYRDSQRMAIASAPDGIIPAGA
                     YETAFRQRTAMARAGQSAANDNRWQPVGNGPLHTMDDGYGEGATGRGDVNGRVNDFFY
                     VPRDDAHLPDTLLATASYGGVWVSDSNASKWTSIGDNLPTQVVSSVAYTPEGGGTIMV
                     LTGDDSFGADSRQGVGLFYTTNLGKTWLAAKGMPRDVWGFKLAVDQARKSIVYAATGS
                     GLYRSTDAGRSFVNVKLPTGECAGQSNRTATCAFANVVTDVIVQMPGGTTDEAGGKVM
                     AAVGWRAGQLKYPGTDIVQSPNNGIYVSDTGAPGTFEKSAATGFAPQDRIGRTELGPA
                     EGPAQNHNYVYAMVQDAVLLRNGPPGIDVPEAYGVVPRVSVFHGLYVSSDFGRTWRIM
                     VSQPELLSPATGSALTAYSVLARYGPGIQAWFNAFVRPDPTKAINGIPTRVVFGLEEV
                     FQNENTNVPQDGHSAFKVIGRYWSGDSCMGLMLPPATTYCATQREGIARTTTTHSDQH
                     DVIFIPFEDGSVRMVVGNDGGVYSQRIAADVSDFSNANWGYGNNEGFNTLLPYDAVRS
                     RDGTFWMGLQDNGTAKITDIVRNGRVIERQRQIQTKGGDGFFVAVHPDNGNIAYGEYT
                     GGTIASTVNGGKTWSEMSPPITSALFSTPFTMDPADPNHLMIGGNEIVSTLAGPGTSR
                     SSWVKLIDLGTHSKPGDEKATPNAQDPVNQQTAVALHGAAGYAGFCGPCDVLNTAVPF
                     KNGIATNVGGSQPPKKGASQGWHIAKAAGLPNRYITGIAIHPSNPEIVFVSLGGYFRP
                     WTKPNVIGAKNSRVGEGHLYVSTDGAKTFRNISANLPNTPISFLTLRPGTNQVVAATD
                     VGVFISVPGGRFEVLGQGLPMVPVHTVRFTNGDPNTLVAAAYGRGVYMYSFGPARAAA
                     AKRVAPAPRFLNKVVAGPWGFETGEDGFTQSSTGAAIEWRRASPGNDSGFSWVLSGYE
                     DSASAILQSPAMNVPARSTIQASWYERHDTEACCDYFSFEWSSDNKNWHQARSIAGQN
                     PTFPGFSKMSARFVVPQGRLWIRWRMTSDSGLSTPPYTGIAIDDVEVRR"
     gene            complement(4020..4562)
                     /locus_tag="VM841_04500"
     CDS             complement(4020..4562)
                     /locus_tag="VM841_04500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89479.1"
                     /translation="MTELSYRPAVAAEAGSVAALINSAYRGESSRAGWTTEADFLVGL
                     RTDEDEIRDLIGHDGSVILLCERADEIIGCVHLRRDGDSAYLGMFVVRPDLQGAGIGK
                     RFMAAAEDLVRREWDARRITMTVLSLRDELIAFYERRGYRRTGRFTPFPADSRSTPMV
                     QDLRFETLEKDLPAAPQPAR"
     gene            4648..5724
                     /locus_tag="VM841_04505"
     CDS             4648..5724
                     /locus_tag="VM841_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HVL89480.1"
                     /translation="MDVRSRHPRLLALLGSGEFEPWAAEVDRRLLEHGTGSGGVLLFA
                     TASAPDGEPVYSAWLRKGSDHYASLGVPVAVADVRTREDARDPAVAGAVAAASLIFFS
                     GGNPAYLATVVRDTPLWSAIAEALERGAAFAGCSAGACLAGDLAPESVTADADAVRWV
                     PALGMLPGTLVVPHWDVLDVYRPGQRTALVNGHARGRTVLGIDEFTAAVIADGVWRVA
                     GEGQVLVQRDVRWRRFSSGAPFDPDAALDHPGPLYHRTFEAARIMRDGFTDTVEERPG
                     GGTARGVWLSDRPLDDEDEIRGDSVLAVEIPYEVAAEFEWTDGGDRYREFCIPAAIVN
                     RSAPFVLVDPRTGPAMNLLPAETG"
     gene            complement(5731..6930)
                     /locus_tag="VM841_04510"
     CDS             complement(5731..6930)
                     /locus_tag="VM841_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89481.1"
                     /translation="MPPRILLLAAALLASLLGPARALPPLPAGSQVIVSSTLNGTLHV
                     FDASTLAETQPPLAVKGTSPVRLWVERFGARALLLSANHGVQGSLGVYDLDTPIVTEL
                     PLSPFASGGQGSVGIAAGTHAATGAAMIFLTDTVQALGGCGLPEGTITGFDARLLATA
                     GVATRFATVSSGGPVPYAVAFDGRRGEVLASNYCNDTFVRLAVAGTGALGDGYGMRPF
                     GTASTGAGPDAVLYDGAYDRAYVTNIRGDSVSVFASGGHTAAVTTVPLPGAGPIDAAL
                     ADSPSGARWMITSNGADDTVSLIDRDAIAACAATAVSPCHAEVARIGAGVPGGAPEGV
                     AYDPATNRIFVVNKTIGAPALSVIQLDESGPAVQGQHIATIPLRVLGAGTPLPDITAF
                     DVVVRPR"
     gene            complement(6968..7984)
                     /locus_tag="VM841_04515"
     CDS             complement(6968..7984)
                     /locus_tag="VM841_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVL89482.1"
                     /translation="MSLSNADLSELLALASGQETGTKHKALRRAAGRALIWPEEAAEI
                     LATGRELTVLPGVGPYLARRIGDWLADPPEVPVPPPLRRGFLTMAIARSVVEAHPEWM
                     PRADLQMHTDETDGSVSLKTMVEACRELGHELIAITDHSQALRITNGMDEARLARQGA
                     AIRRINARGEAPLVLRGIEMNLTPEGEGDMEPSSLRSLDLVLGAFHSKLRVEGDQTQR
                     YLKALRNPHIHVLAHPRGRVFNFRTGLDADWERVFAEAERQGKALEIDATVMRQDLDV
                     ELLMEAKRYDVRFTIGSDAHHPNELHYLTLGAAAAILAGIPRERILNFRGAAGWPVSQ
                     PVPA"
     gene            8101..>8521
                     /locus_tag="VM841_04520"
     CDS             8101..>8521
                     /locus_tag="VM841_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVL89483.1"
                     /translation="MNESSIASARPGAAGLAAATPASRDRVADFLRLAAIVVVVAGHW
                     LMAVVTWQDGRFAGENALETIPHAWALTWLLQVMPVFFFVGGFADAVAWDARRRRGEG
                     YGAYLSSRLERLMRPAAAFVAAWMAAAVALAAAGATET"
BASE COUNT         1271 a         2990 c         2990 g         1270 t
ORIGIN      
        1 gcagcgggat cggcttcggg gccggtaccc tcgctatcgc ggcgcgcgac gttccgacgg
       61 ccgagggggc ctacggaacc attctgatcg acaccgtcgg gatggttcat gacaagccct
      121 accgaaacgt catcgtcggt gcgcttgcgt gtgtcgtcgt cgagccggtc gcggccacgg
      181 gcgggcaccg gatctccaca tacagcttcg cagtcgatac gttcggtgtc tggccacttt
      241 tcgtcaccct ggacgtcgga ccggacggct cggcgtcctt ctcggcgatc cgcggctaca
      301 cgacgccgga cggaccctgc cgggggcccg agtggacgaa cgtgccgctc atcggatcca
      361 cggtgttggt tcgcgccggg cactttcctt ccaccggcat cggtcgaggg tgagccgtca
      421 gaaagaaaaa acgggtgaaa gttcgtaaac cgggccgcgt cccgatcctc gcagaaggaa
      481 cgaaaccgag ccaagcgaac tctcccgatt ggacgtgtgc ggcacgtcct ttcttggcga
      541 tctcggagga cctcgtgaag acccatcttg ccgtccggcg actggcgctg cccgccctcg
      601 ccgcgctcct cgtctcgacc ctcgtgtcct ggcccggggc tcgcaccgac acccaggctc
      661 tggcctgccc gcccggctac cacctcgaag gggaggagga ggcccgtctc tcgctctacg
      721 gggacgtgct cgacgtgcga accggcaccg agctgtgcat ccacgacaag catcccgaga
      781 ccgagcggga acttcgctac cgcgactcac agcgcatggc gatcgcctcg gcgccggacg
      841 ggatcatccc ggcgggcgcc tacgaaacgg cattccgcca gcgcaccgcc atggcgcgcg
      901 cgggacagtc ggcggccaac gacaaccgct ggcagccggt cggcaacgga ccgctgcaca
      961 ccatggatga cggatacggc gagggggcaa ccggccgcgg tgacgtgaac ggtcgcgtca
     1021 acgacttctt ctacgtgccg cgggacgacg cgcacctgcc ggacacgctg ctcgcgacgg
     1081 cgtcctacgg cggcgtgtgg gtctcggact ccaacgcgtc gaagtggacc tcgatcggcg
     1141 acaacctgcc gacccaggtc gtctcgtcgg tcgcctacac gcccgagggt ggcgggacga
     1201 tcatggtgct caccggagac gactccttcg gcgcggactc gcgccagggc gtgggcttgt
     1261 tctacacgac caacctcggc aagacttggc ttgccgccaa gggcatgccg cgggacgtgt
     1321 ggggcttcaa gctcgcagtg gaccaggcgc gcaagagcat cgtctacgcc gcgaccggca
     1381 gcggcctgta ccgctcgacc gacgcgggcc gctcgttcgt gaacgtcaag cttcccaccg
     1441 gtgagtgcgc cggccagtcc aaccggaccg ccacgtgcgc gttcgccaac gtcgtcaccg
     1501 atgtgatcgt gcagatgccg ggcggaacca ccgacgaggc gggcggcaag gtcatggccg
     1561 cggtcggctg gcgcgccggg cagctcaagt atccgggcac cgatatcgtg cagtcgccca
     1621 acaacggcat ctacgtctcg gacacgggcg cgcccggcac gttcgagaag tcggcggcca
     1681 ccggcttcgc gccgcaggat cgcatcggcc ggaccgagct cggtccggcg gaaggtccgg
     1741 cgcagaacca caactacgtg tacgcgatgg tgcaggacgc ggtgctgctt cgcaacggcc
     1801 cgcccggcat cgacgtgccc gaggcctacg gcgtggttcc gcgtgtgagc gtcttccacg
     1861 gcctctacgt gtccagcgac ttcggccgca cctggcggat catggtgtcg cagcccgagc
     1921 tgctctcgcc cgcgacgggc tcggcgctga cggcgtattc ggtgctggcg cgctacggcc
     1981 cggggatcca ggcgtggttc aacgcattcg tgcgccccga cccgaccaag gcgatcaacg
     2041 gcatcccgac gcgtgtggtc ttcggtctcg aggaggtctt ccagaacgag aacaccaacg
     2101 tgccgcagga cgggcactcc gcgttcaagg tcatcggccg gtactggagc ggtgacagtt
     2161 gcatgggtct gatgctgccg cccgcgacga cctactgcgc gacgcagcgc gaaggcatcg
     2221 cgcgcacgac aacgacgcac tccgaccagc acgacgtcat cttcatcccc ttcgaggacg
     2281 gcagcgttcg catggtggtc ggcaacgacg gcggcgtcta cagccagcgt atcgcggccg
     2341 acgtgtcgga cttctccaac gccaactggg gctacggcaa caacgaaggg ttcaacacgc
     2401 tgcttcccta cgatgccgtg cgctcgcgcg acggcacatt ctggatgggt ctgcaggaca
     2461 acggcaccgc caagatcacc gacatcgtcc gcaacggccg ggtcatcgag cgccagcgcc
     2521 agatccagac caagggcggg gacggcttct tcgtggcggt gcatcccgac aacggcaaca
     2581 tcgcctacgg cgagtacacg ggcggaacga tcgcctcgac cgtcaacggc ggcaagacct
     2641 ggagcgagat gtcgccgccg atcacctcgg cgctgttctc gacgccgttc acgatggacc
     2701 cggcggaccc gaaccatctg atgatcggcg gcaacgagat cgtgtcgacc ctggccggtc
     2761 ccggaacatc ccgctcctcg tgggtcaagc tgatcgacct cggcacgcac tcgaagccgg
     2821 gtgacgaaaa ggcgacgccg aacgcgcagg acccggtgaa ccagcaaacg gcggtcgcgc
     2881 tgcacggcgc cgccggctac gccggtttct gcggcccgtg cgacgttctc aacaccgcgg
     2941 ttcccttcaa gaacggcatc gcgaccaacg tgggcggctc gcagccgccg aagaagggcg
     3001 catcgcaggg ctggcacatc gccaaggccg cgggcttgcc gaaccgctac atcaccggca
     3061 tcgccatcca cccgagcaac cccgagatcg tgttcgtatc gctcggcggc tacttccggc
     3121 cgtggacgaa gcccaacgtc atcggtgcca agaacagccg cgtgggtgag gggcacctgt
     3181 acgtgtccac cgacggcgcg aagacgttcc gcaacatctc ggccaacctg cccaacaccc
     3241 cgatcagctt cctgacgctg cgtccgggaa cgaaccaggt cgtggccgca accgacgtcg
     3301 gcgtgttcat ctcggtgccc ggcggccggt tcgaggtgct gggccagggg ctgccgatgg
     3361 tgccggtgca cacggtgcgc ttcaccaacg gcgaccccaa cacgctcgtg gcggccgcct
     3421 acggccgcgg cgtctacatg tactcgttcg gtcccgcccg cgctgccgcg gccaagcgcg
     3481 tggctccggc tccgcggttc ctgaacaagg tcgtcgccgg cccctggggc ttcgagaccg
     3541 gcgaggacgg gttcacgcag tcctcgaccg gggcggcgat cgaatggcgg cgcgcctcgc
     3601 ccggcaacga ctccggcttc tcgtgggtgc tgtcgggcta cgaggacagc gcgtcggcga
     3661 tcctgcagtc gccggcgatg aacgtgccgg cgcgcagcac gatccaggcg agctggtatg
     3721 agcgtcacga caccgaggcc tgctgcgact acttctcgtt cgagtggtcc tcggacaaca
     3781 agaactggca tcaggcgcgc tcgatcgccg gtcagaaccc cacgttcccc ggcttctcga
     3841 agatgtcggc gcgtttcgtg gtgccgcagg gcaggctgtg gatccgctgg cggatgacgt
     3901 cggattccgg tctgtccaca ccgccctaca ccggcatcgc gatcgacgac gttgaggtcc
     3961 gccgttaagg cggcacggaa cttcgtcgcg gcggctcctt cgggggccgc cgcttcgcgt
     4021 cagcgggccg gctgcggcgc ggcgggcagg tccttctcga gcgtctcgaa gcgaagatcc
     4081 tgcaccatcg gggtgctgcg cgagtcggcg gggaagggcg tgaagcggcc ggtgcgtcgg
     4141 tagccgcggc gctcgtagaa cgcgatcagc tcgtcgcgca gcgagagcac cgtcatggtg
     4201 atgcgccggg cgtcccattc gcggcgcacc agatcttcgg ccgcggccat gaaacgcttg
     4261 ccgatgccgg cgccctgcag gtccggccgg accacgaaca tgcccaggta ggccgagtcc
     4321 ccgtcccgtc gcagatgcac gcagccgatg atctcgtccg ctcgctcgca cagcaggatc
     4381 accgatccgt cgtggccgat cagatcgcgg atctcgtctt cgtcggtgcg cagcccgacg
     4441 aggaagtccg cctcggtcgt ccagccggcg cggctggact cgccgcgata cgcagagttg
     4501 atcagcgccg cgacggagcc cgcctcggcg gcgacggcgg gccggtagga aagctcggtc
     4561 atcgcgcaca ggataaggat cggacgcggc ggcggcgagc ggctttcgga atggacggcg
     4621 ctcccgcggg gaacatttct gcccggcatg gatgttcgct cacgccatcc gcggctgctc
     4681 gcgctcttgg gctcgggcga gttcgagccg tgggccgccg aggtcgaccg ccgcttgctc
     4741 gagcacggca ccggcagcgg cggtgtgctg ctgttcgcga ccgcgagcgc ccccgacggc
     4801 gagcccgtct actcggcctg gctgcgcaag ggctcggacc actacgcgag cctgggcgtg
     4861 cccgtggcgg tcgccgacgt gcgcacgcgc gaggatgccc gcgacccggc cgtggccggc
     4921 gcggtcgcgg ccgcgagcct gatcttcttc tccggcggca atcccgcgta tctggcgacg
     4981 gtcgttcgcg acacgccgct gtggtcggcg atcgccgagg ccctcgagcg tggtgcggcc
     5041 ttcgccggat gcagcgccgg cgcgtgcctg gcgggggacc tcgctcccga gtcggtgacc
     5101 gccgacgccg acgcggtccg ctgggtgccg gcgctcggca tgctgccggg cacgctggtc
     5161 gtgccccact gggacgtgct ggacgtatac cggccggggc agcgcacggc gctcgtcaac
     5221 gggcatgcgc gcggccggac ggtgctggga atcgatgagt tcaccgccgc cgtgatcgcg
     5281 gacggcgtct ggcgcgtcgc cggcgaaggg caggtgctcg tgcagcgcga cgtgcgctgg
     5341 cggcgtttct cgtccggggc gccgttcgat cccgacgccg cgctcgacca tcctggaccg
     5401 ctgtatcacc ggacgttcga ggcggcgcgc atcatgcgcg acggcttcac cgataccgtt
     5461 gaggagcgtc ccggtggagg gacggcgcgt ggcgtgtggc tgtcggaccg gccgctggac
     5521 gacgaggacg agatccgggg cgactcggtg cttgccgtcg agatccccta cgaggtggca
     5581 gccgagttcg agtggaccga cgggggggac cggtatcgtg agttctgcat ccccgccgca
     5641 atcgtcaatc gctccgcgcc gttcgttctg gtcgatccgc gcaccggacc ggccatgaac
     5701 ctgcttcctg ccgagaccgg ctgacgcgcg tcagcgcggc cgcaccacga catcgaaggc
     5761 ggtgatgtcc ggaagcggcg tgcccgcgcc caggacgcgc agcggaatcg tcgcgatgtg
     5821 ctgtccctga acggccgggc cgctctcgtc gagctggatc acagacaagg cgggcgcccc
     5881 gatggtcttg ttgaccacga agatccggtt ggtcgccgga tcgtaggcga cgccctcggg
     5941 cgcgccgccg ggcacgcccg cgccgatgcg cgcgacctcg gcgtggcacg gcgagacggc
     6001 cgtggccgcg cacgccgcga tcgcgtcgcg gtcgatgagg ctgacggtgt cgtcggcgcc
     6061 gttggacgtg atcatccatc gcgcccccga cggcgagtcc gcgagcgccg cgtcgatcgg
     6121 cccggcgccc ggcagcggaa ccgtcgtcac ggccgcggtg tgccccccgc tcgcgaacac
     6181 gctcaccgaa tccccgcgga tgttcgtgac gtaggcacgg tcgtaggcgc cgtcgtagag
     6241 gaccgcgtcc gggcccgcgc cggtcgacgc cgtgccaaag ggccgcatcc cgtagccgtc
     6301 gccgagcgcg cccgttcccg cgacggccag ccgcacgaag gtgtcgttgc agtagttgct
     6361 cgccaggacc tcgccgcggc ggccgtcgaa ggccaccgcg tacggcaccg ggccgcccga
     6421 agacaccgtc gcgaagcgcg tcgcaacgcc tgccgtcgcc agcaggcgtg cgtcgaagcc
     6481 ggtgattgtg ccctccggca agccgcagcc gccgagcgcc tggaccgtgt cggtcaggaa
     6541 gatcatcgcg gcgcccgtgg cggcgtgcgt gccggccgcg atcccgacgc tgccctggcc
     6601 gcccgaggcg aacggcgaca gcggcagctc ggtcacgatc ggggtgtcga ggtcgtagac
     6661 gcccagcgat ccttggacgc cgtggttggc cgacagcagc agcgcgcgcg cgccaaaccg
     6721 ctcgacccac agccgtaccg gcgaggtgcc tttgaccgcg agcggcggct gcgtctcggc
     6781 cagcgtcgac gcgtcgaaga cgtgaagcgt tccgttgagc gtgctggaca cgatcacctg
     6841 cgatcccgca ggcagtggtg gcagcgcccg ggcgggaccg aggagcgagg cgagcagcgc
     6901 ggcggcgagc agcagaatcc gaggcggcat ggccgctagt tcttcgcgtg cgcgccggct
     6961 tcctgcctca ggccgggacc ggctgggata cgggccaccc ggcggcgccg cggaagttca
     7021 ggatccgctc gcgcggaatg cccgccagga tcgcggcggc cgcgccgagc gtgaggtagt
     7081 ggagctcgtt gggatggtgc gcgtccgatc cgatcgtaaa tcgcacgtcg tagcgcttcg
     7141 cctccatcag cagctcgacg tcgaggtcct ggcgcatcac cgtcgcgtcg atctcgagcg
     7201 ccttgccctg ccgctcggct tcggcgaaaa cgcgctccca atcggcgtcg aggccggtcc
     7261 gaaagttaaa cacgcgcccg cgcgggtggg ccagcacgtg aatgtgcggg ttgcgaagcg
     7321 ccttgaggta tcgctgcgtc tgatcgcctt cgacgcgaag cttcgaatgg aacgcgccca
     7381 gcaccagatc cagcgaccgc agcgacgagg gctccatgtc gccctcgcct tcgggtgtga
     7441 ggttcatctc gatgccgcgc agcaccagcg gcgcctcgcc gcgcgcgttg atgcgccgga
     7501 tcgccgcgcc ctggcgcgcg agacgcgcct cgtccatgcc gttggtgatt cgaagcgcct
     7561 gcgagtgatc ggtgatggcg atcagctcgt gcccgagctc ccggcacgcc tcgaccatcg
     7621 tcttcagcga gaccgagccg tccgtctcgt cggtgtgcat ctgaaggtcg gcgcgcggca
     7681 tccattcggg atgggcctcc acgaccgagc gcgcgatggc catcgtcagg aacccgcgac
     7741 gcagcggcgg cggcaccgga acctcgggcg ggtcggcgag ccagtcgccg atgcgccggg
     7801 cgaggtaggg cccgacgccg ggcaggacag tgagctcgcg gccggtggcg aggatctcgg
     7861 ctgcctcctc gggccagatc agcgcgcgtc cggccgcgcg gcgcagcgcc ttgtgcttgg
     7921 tgccggtctc ctgcccggaa gcgagcgcga gcagctcgga cagatccgcg ttcgacaagc
     7981 tcatgccgtg atgactaccc gcggctgccg aaagtaccca ggcgaacctg ggcgagcgcg
     8041 ccgtcgatat gagcgcgatg ccgtctaccc gccgcgcggc ccggccgcca ggatgagccc
     8101 atgaacgaat cctcgatcgc ctccgctcgt cccggcgccg ccggcctggc ggccgcaacg
     8161 cccgcatccc gcgaccgcgt cgccgacttc ctgcggctcg ccgcgatcgt ggtggtcgtc
     8221 gccggccact ggctgatggc cgtggtcacc tggcaggacg gccgcttcgc cggcgagaac
     8281 gcgctcgaga cgattccgca cgcctgggcg ctgacctggc tgctgcaggt gatgccggtc
     8341 ttcttcttcg tcggcgggtt cgcggacgcg gtcgcgtggg acgcgcggcg ccgccgcggc
     8401 gagggctacg gcgcctacct gtcgtcgcgg ctggagcgtt tgatgcgccc ggccgcggcg
     8461 ttcgtcgcgg cctggatggc cgccgcggtc gcgctggccg ccgccggggc gaccgagacc
     8521 t
//
LOCUS       DATLFO010000122         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_12305, whole genome shotgun sequence.
ACCESSION   DATLFO010000122 DATLFO010000000
VERSION     DATLFO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12305"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VM841_04525"
     CDS             <1..667
                     /locus_tag="VM841_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVL89484.1"
                     /translation="TASSIDSSEHMAERMPWFFLAVLALSFLLLMVVFRSLLVPLKAV
                     LMNLLSIGSAYGIVVALFQWGWLSDLTGVQPAPIEPWAPMMLFAIVFGLSMDYEVFLL
                     SRVREEWRRTGDTKTSVADGLAATAKVITAAAAIMVVVFGSFILEADRVVKLMGTGLA
                     IAILLDATIVRMILVPATMELLGDRNWWLPKWLDRILPRVDVEGSASIEPGGEPAPAE
                     VRD"
     gene            complement(689..1897)
                     /locus_tag="VM841_04530"
     CDS             complement(689..1897)
                     /locus_tag="VM841_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVL89485.1"
                     /translation="MIFVGIDWAEAHHDACIVDEAGAVLGKQRVPDGVEGIGRLHAMI
                     ADHAEDPAQVVVGIEIDRGLLVEALVSAGYEVFAINPMAASRYRDRHATSRAKSDPGD
                     AKVLADLVRTDRHNHRRLAVAGDAAIKVLARAHQDLIWSRQRQVNHLRNQLREFYPAA
                     IAAFGSDLAHPDALAVLERAPSPAQGRALSASQIASALRKGGRQRNLDKRAGEIKDAL
                     RTPQLEAPRLVADAYAASVAATMPVIRSMTEQIERLAAELSKSFRSHPDAEIYLSLPG
                     LGDVLGARALAEFGDDRTRYANPKGRKAYAGTAPITRASGTRLIVLARLARNKRLADA
                     CYLWAFAALNSSRGCRRYYDAHRSRGKTHHQALRAVANRLVGILHGCLEQQTLYREDV
                     AWPPQLEAAA"
     gene            complement(2120..>2367)
                     /locus_tag="VM841_04535"
     CDS             complement(2120..>2367)
                     /locus_tag="VM841_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL89486.1"
                     /translation="ANTFVPSQTLPSVLRIAAEWNPVSAAVQAARNGFGNNVAATAVS
                     DAWSLNHPVLYTVICVGVILAIFVPLSVRQYERAALR"
BASE COUNT          371 a          790 c          793 g          413 t
ORIGIN      
        1 cacggccagc agcatcgact cgtcggagca catggccgag cgcatgccgt ggttcttcct
       61 cgccgtcctg gccctgtcgt tcctgctgct gatggtcgtc ttccggtcgc tgctcgtccc
      121 gctcaaggcc gtcttgatga acctgttgtc gatcgggtcc gcgtacggca tcgtcgtggc
      181 cctcttccag tggggatggc tgtcggacct caccggcgtg cagcctgcgc cgatcgaacc
      241 gtgggcgccg atgatgctct tcgcgatcgt cttcggcctg tccatggact acgaggtatt
      301 cctgctctct cgcgtgcgtg aggagtggcg gcgcaccggc gacacgaaga cctcggtcgc
      361 cgacgggctc gccgcgacgg cgaaggtgat caccgcggcc gccgcgatca tggtcgtcgt
      421 gttcggctcg ttcatcctcg aggccgaccg ggtcgtcaag ctcatgggaa ccggcctggc
      481 gatcgcgatc ctgctcgatg cgacgatcgt gcgcatgatc ctcgtcccgg ccacgatgga
      541 gctcctcggc gaccggaact ggtggctgcc gaagtggctc gacaggatcc tgccgcgtgt
      601 agacgtcgaa gggtctgctt cgatcgagcc gggcggcgag cccgcgcctg ccgaggtccg
      661 cgactagaca tcccacggtc ctaacgtgtc aagccgcggc ttcgagttgc ggcggccagg
      721 cgacatcttc ccggtagagg gtttgctgct cgaggcagcc gtgcaggatc cccacgagcc
      781 ggttcgcgac ggcgcgaagc gcttgatggt gcgtcttgcc gcgcgatcga tgggcgtcgt
      841 agtagcggcg gcaaccgcgt gagctgttga gcgcagcgaa ggcccagagg tagcaggcgt
      901 cggcgagccg tttgtttcga gcgagccgtg cgagcacgat caggcgtgtc cccgaggctc
      961 gcgtgatcgg cgcggtgcct gcgtaggcct tgcgaccctt aggattggca tagcgggttc
     1021 ggtcgtcgcc gaactcagcc agcgcccggg cgccgaggac atcgccgagt ccaggcagac
     1081 tgaggtagat ctcggcgtcc gggtggctcc taaaactctt cgacaactcc gcggccagcc
     1141 gttcgatctg ctcggtcatc gatcggatca ccggcatcgt ggcggcgacg cttgccgcat
     1201 aggcatcagc cacgagccgc ggggcttcca gctgtggggt gcgcagtgcg tctttgatct
     1261 cgcccgcacg cttgtcgagg ttgcgctggc ggcctccttt gcgcagagcc gatgcgatct
     1321 gagaagccga tagcgcacga ccttgcgccg gtgacggcgc acgttccagc accgccagtg
     1381 cgtcaggatg cgcaagatcg ctgccgaacg ccgcgatcgc agcgggatag aactcgcgga
     1441 gctggttgcg gagatggttc acctgccgct gacgggacca gatcagatcc tgatgcgctc
     1501 tggccagcac cttgatcgcc gcatcgccgg cgacagcgag gcggcgatgg ttgtggcggt
     1561 cggtgcgcac cagatcggcc agcaccttgg cgtcgccggg gtcactcttg gctcgcgagg
     1621 tcgcgtgacg atcgcgatag cggctggcgg ccatcgggtt gatcgcgaag acctcgtaac
     1681 cggctgagac caacgcctcg acgagcagac cccgatcgat ctcgatgccg accacgacct
     1741 gcgcaggatc ctcggcgtgg tcggcgatca tcgcgtgcag gcgaccgatg ccctccacgc
     1801 cgtcaggaac ccgttgcttg cctaggaccg caccggcctc gtcgacgatg cacgcgtcat
     1861 ggtgcgcctc agcccaatcg ataccaacga agatcacccc gtcgtctcct ctccctcgac
     1921 gtcgagcccg aggagacctg cggcgcgcta atggatcagt gctcgaggca cgacatccca
     1981 ccagccgtcc agcctcctca ccgaccagcc ggggcactct ctagtcgtag agctcgaggc
     2041 cctgccgctc tcagtgctca ccggctggcg gctcggagcc agtctctcat caagaccggt
     2101 cgagtcccat tagccgcgcc tagcgcaacg ccgcgcgctc gtactgccgc acggacagcg
     2161 ggacgaagat cgcgaggatc acgccgacgc agatgaccgt gtagagcacg gggtggttca
     2221 gcgaccacgc atccgacacc gcggtcgccg ccacgttgtt gccgaagccg ttgcgcgcgg
     2281 cctgcaccgc ggccgacacg gggttccact cggcggcaat acggagcacc gagggaagcg
     2341 tctgggacgg cacgaaggtg ttcgcga
//
LOCUS       DATLFO010000123         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_44999, whole genome shotgun sequence.
ACCESSION   DATLFO010000123 DATLFO010000000
VERSION     DATLFO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44999"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..314)
                     /locus_tag="VM841_04540"
     tRNA            complement(242..314)
                     /locus_tag="VM841_04540"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Ala,seq:cgc)
     gene            469..708
                     /locus_tag="VM841_04545"
     CDS             469..708
                     /locus_tag="VM841_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89487.1"
                     /translation="MDRSSNKHGPRIDDEIRKETDSITKGSPAETRVDAARRKEDPGD
                     DQPNVEQVLNRTGHVPEHFDPRREATAHGSRPDVP"
     gene            complement(806..>3246)
                     /locus_tag="VM841_04550"
     CDS             complement(806..>3246)
                     /locus_tag="VM841_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HVL89488.1"
                     /translation="TNIFYFGNRIHDVAYRYGFTEKFGNMQSENFDRGGRGHDAVRFM
                     AMAGVLNGQFDNAFMFTPADGGEPQDLIDTAEPARTIGRATSFIPPFSGMFLWKPVPT
                     FQAPCVDGDFDASTAYHEYAHGISNRMTGGGDDADALSAHQSGSMGEAWSDWIAHMIL
                     EQEGLEDRTADGIYITGNTITGIRHFALDKNPLTYGDIGFGRFGPEVHDDGEIWSGTL
                     WDLKVALVAKQGKARGIDKAMRLVFDGMAMQTRLPDMLDGRDAILKANQVRYKGADAA
                     LIWDVFARRGMGRSARSIDANDVNPRPGFDSVTPNGYLAGTIRDIGGSGMIAKVLVGL
                     GEGKPTPVAVTPRSGQFRIPMTPGRYTVTYAAAGYGLQKIGTVVVSAGRTTTIRPIAL
                     NRNLASAGWGALIAGGLAAGDPGTALLDDHEFTGQIVEVGKPVTIMLGGTGPATVRGI
                     AVSTKPAGGGTSQNATGYKVETSIDGRSWRTALTGGVKRYKPRPIAMHWTRTTKGMNV
                     QARYVRVTVTSALANTRAILSGIQVLGSAPGMAVAPVGGGKPFVDSDGSVAVANAAGP
                     DASITYNEFSLGCMTPLSQGLDAYVVALPRGAGDGVHNFATKGGQELARDLDAYFYGP
                     DCSMTGAIASPSADEAGPIPPGSAWAVIVLFAGTTDGFKVEVTSTLERVIPPPGTPTR
                     TPRLPAAGNRTAEQSYIATGGDLLSTACFGDPLPEPYVGGGCFEVRSGERTVKVTVHD
                     DEFANPSGFWAFRDADGNMIGDVETPFCGSFTVSVPRGAAELSIWLDQAFAFSSDCEG
                     DPATTGTIKAVFSS"
BASE COUNT          531 a         1076 c         1122 g          517 t
ORIGIN      
        1 ggcccagcga ggccgaggat gaccgatcgc gatccgggcg acatcgaaca gcatggattc
       61 aacgaccagc ttcttggcct cggcgtcccc ggcccgcgcc gatcccaaca gccgcgccgc
      121 tccggctggt tgcggcgcga gaaattctcc acttccgcga gatactgctc aagagccttg
      181 cccggatcca tcatcgccgc gaatcgtact tcgcgtcgtc acgaggaacc aggtttatcg
      241 gtggaggtga acggattcga accgtcgacc tctgcgttgc gaacgcagcg ctctaccaac
      301 tgagctacac ccccgagatc gaaggagcat ggtagcaaac caggtggccg ggcgaacggc
      361 cggcacggcg acgggaaggg gtccggcaat tcgggaatcg gaccgagcgg aagggcccgg
      421 ttagcgcggc aagcatcccg ggaatcgcca tagctgaggt gatgcccgat ggatcgctca
      481 tcgaacaagc acggcccgcg cattgacgac gagatacgca aggaaaccga ctcgatcacg
      541 aagggatctc cggccgagac ccgggtcgac gcagcgcgcc gcaaggagga cccgggtgac
      601 gaccagccca acgtggagca ggtgctcaac cggaccggcc acgtccccga gcacttcgat
      661 ccgcggaggg aagcgacggc acacggcagc cggccggacg tcccttgagg aagccggccg
      721 gagcgggaaa gggctgtcaa cagggttcgt tggtcatatg actccgatat ccggagtcat
      781 atgaccaaca aacaaccaac gccgtctagg agctgaacac ggctttgatg gtgccggtgg
      841 tggccggatc gccttcgcag tcgctggaga acgcgaacgc ctggtcgagc cagatcgaca
      901 gttcggccgc gccgcgcggt accgagaccg tgaacgagcc gcagaacggc gtctcgacgt
      961 cgccgatcat gttgccgtcc gcatcgcgga acgcccagaa cccgctcggg ttcgcgaact
     1021 cgtcgtcgtg cacggtcacc ttgaccgtcc gctctccgga gcgcacctcg aagcagccgc
     1081 cgcccacgta gggctcggga agcggatcgc cgaagcaggc ggtgctcaga agatcgccgc
     1141 ccgtcgcgat gtaggactgc tcggcggtgc ggttgcccgc ggccggcagg cgcggcgtgc
     1201 gcgtcggtgt gcccggtggc gggatgacgc gctcgagcgt cgaggtgacc tcgaccttga
     1261 atccgtcggt ggtgccggcg aagagcacga tgaccgccca tgccgagccc ggcgggatcg
     1321 gtccggcctc gtccgccgac ggcgaggcga tcgcccccgt catggaacag tccgggccgt
     1381 agaagtaggc gtcgaggtcg cgggcaagct cctgcccgcc cttggtcgca aagttgtgca
     1441 cgccgtcgcc cgcgccgcgc ggcaacgcga ccacgtacgc gtcgaggcct tgcgacagcg
     1501 gcgtcatgca gccgagcgag aattcgttgt aggtgatgct cgcgtcggga ccggccgcgt
     1561 tcgcgacggc gaccgagccg tcggagtcga cgaagggttt accgccgccg accggcgcga
     1621 ccgccatgcc gggcgcactg ccgagcacct ggatgccgct caggatcgcc ctcgtgttcg
     1681 ccagcgccga ggtgacggtc acgcgcacgt accgcgcctg cacgttcatt cccttggtgg
     1741 ttcgcgtcca gtgcatcgcg atcggacgcg gcttgtagcg cttcacgccg cccgtcagcg
     1801 cggtccgcca gctgcgtccg tcgatcgagg tctcgacctt gtagccggtc gcattctgcg
     1861 aggttccgcc acccgcgggc ttggtcgata cggcgatgcc gcgaaccgtc gccggtccgg
     1921 tgccgccgag catgatcgtc acgggcttgc cgacctcgac gatctggccg gtgaactcgt
     1981 ggtcgtcgag cagcgcggtg ccgggatcgc ccgctgcgag gccgcccgcg atcagcgcgc
     2041 cccatccggc gctcgccagg ttccggttca gcgcgatcgg cctgatggtg gtggtcctgc
     2101 ccgccgacac gaccacagtg ccgatctttt gaagcccgta gccggcggcc gcgtaggtga
     2161 cggtgtagcg gccgggtgtc atcgggatgc ggaactggcc gctgcgcggg gtgaccgcga
     2221 ccggggtggg ctttccttcg ccgaggccga ccagcacctt ggcgatcatg ccgcttccgc
     2281 cgatgtcgcg gatcgtgccg gcgagatagc cgttgggggt aaccgagtcg aagccgggcc
     2341 gcgggttgac gtcgttggcg tcgatcgagc gcgcgctgcg gcccatgccg cggcgcgcga
     2401 acacgtccca gatcagcgcc gcgtcggcgc ccttgtagcg aacctggttg gccttgagga
     2461 tcgcgtcccg cccgtcgagc atgtcgggaa gccgcgtctg catcgccatg ccgtcgaaca
     2521 ccaggcgcat cgccttgtcg atgccgcgcg ccttgccctg cttggcgacg agcgcgacct
     2581 tcaggtccca cagcgtcccg gaccagatct cgccgtcgtc gtgcacctca ggtccgaagc
     2641 ggccgaagcc gatgtcgccg taggtaagcg gattcttgtc gagcgcgaag tgacggatgc
     2701 cggtgatcgt gttgccggtg atgtagatgc cgtcggcggt acggtcctcg aggccttcct
     2761 gctcgaggat catgtgcgcg atccagtcgc tccacgcctc gcccatcgag ccggactggt
     2821 gcgcgctgag cgcgtcggcg tcgtcgccgc caccggtcat gcggttcgag atgccgtgcg
     2881 catactcgtg gtaagccgtc gaggcgtcga agtcgccgtc cacgcacggc gcctggaacg
     2941 tgggcaccgg cttccacaag aacatgcccg agaacggcgg gatgaagctc gtcgcgcggc
     3001 cgatggtgcg ggccggctcg gccgtgtcga tcagatcctg cggctcgccg ccgtcggcgg
     3061 gcgtgaacat gaacgcgttg tcgaactggc cgttgagcac gccggccatc gccatgaaac
     3121 gcacggcgtc gtggccgcga ccgccgcggt caaagttttc ggactgcata ttgccgaact
     3181 tctcggtgaa gccgtagcgg taggcgacat cgtggatcct gttgccgaaa tagaagatgt
     3241 tggtga
//
LOCUS       DATLFO010000124         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_83382, whole genome shotgun sequence.
ACCESSION   DATLFO010000124 DATLFO010000000
VERSION     DATLFO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83382"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VM841_04555"
     CDS             complement(<1..1188)
                     /locus_tag="VM841_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89489.1"
                     /translation="MKSRRGIFAALLVVVVAAPMHVLAGPEDDAMYILTVYDFAGQST
                     TVRAPYMTPVPVDSNFDGIYDRTVTVGGVPRTGSVLSANELRPVRSIVNPLWQAASDH
                     VQSVLREGQRLTQHVPSWVWQAVPEVGELPAGVESLFIQSAPITPSNPHRAVTVGRFR
                     PHGISVGIGVAAGFMQIGYEMSESYETFQIRAIRAHDPSGDTTTTVNIEAAGASAIGS
                     IVLKTGFLTASTVDDARLTLGFAGAEQSRSASLTLRQAGSVTTATMRAPAQSVSAVLS
                     RTTPGETFDFRITVPDFPDDVTFVHTPGAIRTLQVISPGISYMRVDGDIASSSDRSQL
                     LAILRDLPPSVRVVISEAYGSALVQPESGSIGEVEVNLSRNEQLPAAPLSQYLPGISG
                     RDYA"
     gene            complement(1325..>2113)
                     /locus_tag="VM841_04560"
     CDS             complement(1325..>2113)
                     /locus_tag="VM841_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89490.1"
                     /translation="ALTYSNSDDTLWLAPQASERAYQFSMTGSYLGHFDTNDSPNALA
                     PGCNGINRINGLSARSDAIYVTGRCTSYFRYSTSGTLLGSVSMSGASEPADIACDDTT
                     FGLPVNWLRDNGGNRIIAIQVEAGSCLAGVPGPLKRATGLYATPAIASVDVYPAIPPR
                     SSHAVYFPNFSAVLYDAATGQAIAGKIIDFFADGLPGLGIGRRWLCSAVTNSGGRATC
                     GGVEPEVYTIVGLGYQAVFAGDAGYQMSLARGTLILALGRALPY"
BASE COUNT          386 a          671 c          673 g          383 t
ORIGIN      
        1 ggcgtagtcg cgtcccgata tgccgggcag atactgcgag agaggcgcgg ccggcagttg
       61 ctcgttcctt gaaaggttga cctcaacctc gccgatggag ccggattcgg gctggacaag
      121 agccgagccg tacgcttcgg agatcacgac gcggaccgag ggcggaagat cccgaagaat
      181 tgccagcaac tggctccgat cggatgagga ggcgatgtcg ccgtcgactc gcatgtacga
      241 gattcccggc gagatgacct gcaaagttcg aattgctccg ggcgtgtgaa cgaaggtcac
      301 gtcgtccgga aaatcaggca cagtgattcg aaagtcgaac gtctcgccgg gggtggtgcg
      361 gctgagaacg gcgctgacgc tctgtgccgg ggctctcatc gtcgccgtcg tgaccgaacc
      421 cgcctgccga agggtcagcg aggccgagcg cgactgttcc gcgcctgcga accccagcgt
      481 tagccgcgca tcgtcaacgg tcgaagccgt caagaaaccg gttttcagaa caatcgagcc
      541 gatcgccgag gcaccggccg cctcgatgtt cacggttgtc gtcgtatcgc cgctcgggtc
      601 gtgcgcacgg atggcccgga tttgaaatgt ctcgtagctc tcactcatct cgtaaccaat
      661 ctgcatgaag cctgcggcga ctccgatccc gaccgatatg ccatggggtc ggaaccgccc
      721 aaccgtcacg gcacggtgcg gattggacgg ggtgatgggc gcgctttgga tgaacaacga
      781 ctcgactccg gcgggaagct ctccaacttc tggaaccgcc tgccacaccc atgacgggac
      841 gtgctgcgtt aaccgctgac cctcgcgaag caccgattgc acatggtcag acgccgcctg
      901 ccagagagga ttgacgatgc tgcgaaccgg tctcaactcg ttcgctgaaa gcactgaacc
      961 tgtccggggc accccgccga ccgtcacggt tcggtcgtag attccgtcga agttcgagtc
     1021 gacgggcacg ggcgtcatgt acggtgcgcg gaccgtcgtc gactgacccg cgaagtcgta
     1081 gacggtcagt atgtacatcg cgtcgtcttc tggtcccgcc agcacatgca tcggtgccgc
     1141 tacgaccacc acgagcagcg cggcgaatat gcctcgcctg gacttcatat cttccccccc
     1201 gagttgtcag acatgatccc ccggaccgac ttcacatcgt tcgccgtgat gtgcacgggc
     1261 cctgagcccc cggctccgac ggcgccccgc tgtcctcgct ttcctttctg tgccgaagag
     1321 cgcctcaata gggaagggct cgccccagcg caaggatgag cgtcccacgc gcaagggaca
     1381 tctgataacc cgcgtccccg gcgaagactg cttggtaccc caggccgacg atcgtgtaga
     1441 cctctggctc gactccccca caggtagccc ggccaccgct attggtgacc gcgctgcaca
     1501 gccatcgcct tccgatgccg agtcccggta gtccatcggc gaagaagtcg atgatcttgc
     1561 cggcgatggc ctgtcctgtt gcggcgtcgt ataggaccgc cgagaagttc gggaagtaca
     1621 ccgcgtgact gctgcgcggc gggatcgccg ggtagacatc gacggatgcg atcgccggtg
     1681 tcgcgtatag ccccgttgct cgtttgagcg gtcccgggac accggccaga cacgagccgg
     1741 cttcgacctg gatggcgatg atgcggttgc cgccgttgtc gcgcagccag ttcaccggca
     1801 atccgaaggt cgtgtcatcg caggcaatgt cggccggctc cgacgcgccg gacatggaca
     1861 ccgatcccag aagcgttccc gatgtcgaat agcggaagta ggaggtgcag cggcccgtca
     1921 cgtagatcgc gtcgctccgc gcggaaaggc cgttgattcg gttgataccg ttgcagcccg
     1981 gtgccagcgc gttcggtgag tcgttcgtgt cgaagtggcc gagatacgaa cccgtcatcg
     2041 agaactgata cgcgcgctcg ctcgcctgcg gcgcgagcca gagcgtgtcg tcgctgttgc
     2101 tgtaggtgag cgc
//
LOCUS       DATLFO010000125        13659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_92869, whole genome shotgun sequence.
ACCESSION   DATLFO010000125 DATLFO010000000
VERSION     DATLFO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92869"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VM841_04565"
     CDS             complement(<1..1155)
                     /locus_tag="VM841_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89491.1"
                     /translation="MIVSLGAGRDASRELRERIALDTAGLDRVLATPRPGIGELVVLS
                     TCHRTELYATTAGIADADAAHVLARLLPAIRPGDQAELRFRRGGEAIEHLLRVASGLD
                     SLVVGEHQILGQVRRAFQTAASYGAAGPVLGSIFSEAIRVGRLTRSRILPGTATASIG
                     ALVAGDLTARLDGLGGRPGAIVGSGEAAADAAIALRTAGARLIVVGRTPATTQALAAR
                     VDGDPRPMDALPAALATSEFAVVAVAGGVLVRPTHIVERTAPLAIVDVSVPRAVAVRD
                     HPHVHVRTLDDLSPVAASAVVSDAETAVRAATAELIRRLDPSGRRRDIAALRAKAERV
                     ARAEAARALGGMDLAPQHAERIEAMARRIAAKLVHGPTLAMRRAIASIRSA"
     gene            complement(1176..2102)
                     /gene="hemH"
                     /locus_tag="VM841_04570"
     CDS             complement(1176..2102)
                     /gene="hemH"
                     /locus_tag="VM841_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HVL89492.1"
                     /translation="MTTGVLLMAYGSPNSIEEIEPYLRDIRGDRPIRDGLLEDLIRRY
                     SAVGVPSPLLDVTLAQAAGIERALSDDGDYRVFVGMKHWEPWIRQAVEEIDASSVRRV
                     IGVAAAPHYSAMSIGGYAGRVERAMKETGARFTFRMIESWWEQPGYSALVADNLRETM
                     HGWDPADPATHVFFTAHSLPERILAAGDPYRDQLLASAERIAQRAGIRHWSFAFQSAS
                     QTGEPWLGPDLLDALSIFAGDGGRRAAVAPIGFVADHLEILFDIDIEAVEAAGRLGVE
                     LRRIPSPNADPRLCRAIADAVLAEEASWRVAS"
     gene            complement(2099..3157)
                     /gene="hemE"
                     /locus_tag="VM841_04575"
     CDS             complement(2099..3157)
                     /gene="hemE"
                     /locus_tag="VM841_04575"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HVL89493.1"
                     /translation="MAAISARETLSSSQSPFLRACRGEPVPYTPIWLMRQAGRTIPAY
                     RALRETYGFLEVIGDPALMAEVTMLPMREMDLDAAVMFADIMLPLASLGVRFEIVESI
                     GPVVDEPIRRRGQVDAMARIPIEESVPTVFEAIRILRRELEGRAPLIGFSGAPFTLAS
                     YLIEGRPSREYAKTKAIMFGAPEVWAALMERLTTLVIAYLRAQITAGVQAVQLFDSWI
                     GALSAHDTRTYVLPYSKRIFAALDGSGVPRIHFGTGTAHMLDVMAEAGPDVVGVDWRI
                     PLDDAWRAIGDLGIQGNLDPAVLLGPPELIETRARDVLRRADGRPGHVFNLGHGVLPD
                     SPLDNIKRLVDFVHEETA"
     gene            3303..3911
                     /locus_tag="VM841_04580"
     CDS             3303..3911
                     /locus_tag="VM841_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL89494.1"
                     /translation="MARTGGEERKRSLVAHAVRLFGSLGYDGTSLDSVAEAAGVRKQT
                     LLYYFPTKEALFEACVEQVGARAAQALEAALEGPEEGFGRAEAVIRTIFRLTEEWPEF
                     PTFIREASRNGPAAVGRIAGTLEPLRKRALVFLEKEMAEGTFRRQDPTLLLFTLYTAV
                     VGSVTEAGVLRAVGGGAVGRDALKRREEELIGFLRVALTGKN"
     gene            3995..4342
                     /locus_tag="VM841_04585"
     CDS             3995..4342
                     /locus_tag="VM841_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVL89495.1"
                     /translation="MGDLEGPLPAATFHILLALTDGPKHGYAIMQRVEDLSDGSVRMG
                     PGTLYGTVKRMVTDGLIEEVAGPDGEDDRRRTYRLTAAGQTSCAAEVRRLSKLLSDAA
                     VKRWSRGLSPGTA"
     gene            4339..5031
                     /locus_tag="VM841_04590"
     CDS             4339..5031
                     /locus_tag="VM841_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89496.1"
                     /translation="MSAPRWYRAVLLLYPRTFRREYGDEMVAVLEDMMAGGARGPGLV
                     TRIVRDVVVSVPRIRREETTMATKAIIAGVLVLTVGLAVAGTGSVALGGIVSGTVLAV
                     VLGGATLLARRGSASTEAAYRAARPVWPVAAAFVGAYALFTAVQQYVSEPTAASRSQL
                     LFGLIAPALVAGGLWARAAGRRWAAIPIVAGCAPLLVVFWFVPVPVAALVAVIGSIAE
                     SMRASSRPRPAV"
     gene            complement(5045..5335)
                     /locus_tag="VM841_04595"
     CDS             complement(5045..5335)
                     /locus_tag="VM841_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89497.1"
                     /translation="MASLKVVASRLEQADAKHDHVELVGYHSLHLAAEPIMLSPERVI
                     QLTALGSRFYIVDGDAEHDVVEGKCPLCGHEPYLRTKGDQGDEQKLMNLPPG"
     gene            complement(5738..7558)
                     /locus_tag="VM841_04600"
     CDS             complement(5738..7558)
                     /locus_tag="VM841_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVL89498.1"
                     /translation="MSLNEYPPIASYALLGDCHSSALVSREGSIDWCCFRRFDSRPVF
                     AAILDRVKGGHFRIAPTDPFTVTRRYIEGTNVLETRFETDGGVIVLTDFLTVESPHRS
                     GPEHTHPYEQLIRLIRCESGSVEIEKHFSPRFEYGLTRPRVLVREPDRAIVFGGADAL
                     VLQTDIGLDQTDISDERIRTTLRAGDERYVVLTHVDPPDLDGIRVEHDVIGRRYRETL
                     EYWRAWSGRCRYDGPWRDQILRSALVLKALTYDPTGAIVAAPTTSLPEQIGGVRNWDY
                     RYTWLRDAALHLRALFRLGYIDEAHAFMQWLQRTAAGTAENLQVLYGVGGERMVPEIE
                     LGHLEGYRGSAPVRIGNGAVDQFQLDMYGELMDTVWLYHRNGGEIDEPFWQFLCSVVS
                     VVADCWREPDRGIWELRGEPRELVASKLFAWLAVDRGIRLAKDSGFAADLERWEALRE
                     EIRDWIETNGVDPATNAFRQSPETDEPDASLLLIPLTGFLPGDDPRVLATIRRVEERL
                     MEDCLVRRYLTDDGLPGGEGAFVICTFWMVGALAKAGEVERATELFENMLGHANDLGL
                     LSEEVDPETGDLLGNFPQAFSHLGLIQAAMNLREASRRSG"
     gene            complement(7613..8386)
                     /locus_tag="VM841_04605"
     CDS             complement(7613..8386)
                     /locus_tag="VM841_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89499.1"
                     /translation="MSRPVAVAPASPPREQRLAQPPPLRLVPASAELPLRVFVIAGGA
                     SISAGLAASALAWPLWSVALITLGPWIPLYLRDVAEVARRNPWLALFYVVAVSQGGHL
                     VEHVTQMVQLHLLHLPPAHSRGIFGPLDVEWVHFGWNTFILLATASLLAAYRHSRLLL
                     AAFALAIWHQAEHTWIMSVYLSTGDAGSAGLLAAGGVIGGGLPLKRADLHFLYNIAET
                     TPLIVAFVIETARAKRTARLQDAARVRRLESRRSAPGTA"
     gene            complement(8512..9795)
                     /locus_tag="VM841_04610"
     CDS             complement(8512..9795)
                     /locus_tag="VM841_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89500.1"
                     /translation="MRRGVRTIVAVAVLALVATACRSSGDGGNGTSGEIKTGVGVTGQ
                     TITLGELSPLTGPVAVIGKPLTKGHEVYFQYVNEVLGGVGKNLPADKKFKVELETLDT
                     QYVPQTHAEQYGKLKDKVLLIAQSLGTPTTKAILEQVRADKILTGAATLSSDWLKEKY
                     VIPAGAPYPVQAINAFAYVVNEQKKTPKVGVIYQDDDYGADGLKGVVYAAGKLNFQIV
                     ARATYKPTDTEFVSQVTAMKNAGADHVFLVTVPSATGGILGAAAQLKYTPQWIGQSPA
                     WIGALAKSPLADYLKAYFWATGDAACDWGSTAAGCDGMKEMVDNLAKYGGGQTPDTYF
                     QFGYTQAKIVHQILEKAITMGDLTREGVLKAFESLSDVDMGGLLNPISYGTKCEDKVP
                     ATGTAIYSVDASAPTGLKRLAAVDDPIVKDFPFCD"
     gene            complement(9858..10946)
                     /locus_tag="VM841_04615"
     CDS             complement(9858..10946)
                     /locus_tag="VM841_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL89501.1"
                     /translation="MNALTSRRPETLHTSYAQSVSIFPTRGAKIGMALLAVVVVVLPF
                     TLTDFWLAVINFAAIASIGAIGLNLLTGFTGQVSLGHAFFIGLGAYTAGYLGGNHGLP
                     FVVWLPAAGILGGIVGLIVGPFALRLKGLYLAIVTLGLVFLGEHLFLNLRDVTGGPQG
                     RSIPAPEVGGISFNAIGTFSRDQSFYLLAIPMLVLMALIAKNIVRSRTGRAFQAIRDR
                     EIAASIIGVNLFRYKVAAFVISSAYAAIGGAMLGSYLRFAAPSQFDLFLSIQYVAMII
                     IGGIGTVFGAILGAFFIALVPRLVEEFSDRLPFVATTATAEGITVFELNQIIFGALII
                     VFLVAEPYGCAGLWRRAKSYFKTWPFSY"
     gene            complement(10943..11830)
                     /locus_tag="VM841_04620"
     CDS             complement(10943..11830)
                     /locus_tag="VM841_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL89502.1"
                     /translation="MTEFLQFVYQGVVLGAVYALIALGFVIVYKSSEVINFAHGELVL
                     LGGSFVFLWRNQIGLPFAVALLLAIVIMAGVGLGIERVVLRRMVGRPVFAIVMITLGL
                     AIVIRQIVTSVWGFADRHIDDPFGATVYDVGALSVTAASIWTLVATGLVLLGFFIFFR
                     SSKSGVAMRAAAFDQEAALSLGISVRRMTAISWAIAGATATVAGVFIATFPKSLSPTV
                     GFVALRAFPAAILGGLDSPGGAVIGGLAIGLIEVLTQAYQPSWLGNSFHVVAPYAVMV
                     VVLMIRPYGLFGTREVERV"
     gene            complement(11827..>13659)
                     /locus_tag="VM841_04625"
     CDS             complement(11827..>13659)
                     /locus_tag="VM841_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL89503.1"
                     /translation="PDEQRRQPAQQPATLPALFVGRARLAPGNVALREKAFGIWREHS
                     WSDYEARVREIALGMVWLGIQPGDRVAILSDDRPEWVFAELGAVTAGATSVGVYPTSP
                     AAEVGYVLEHSGARVVVAENQEQVDKILEVKLPSLERIVVIDRRGLRGYDDPRLMPFE
                     DVLSAGAQMHAADPSHFERLVSERTPDEIAMIVYTSGTTGPPKGAMLSQANLAASIAL
                     TKSAWDEITDGDRVLSYLPLNHVAEKIFTIYFPIAFGVSASFAESIDTVPENLREVEP
                     TIFLGVPRIWEKMAAGIEIRMRDASPVKRALFRFWLKRGQTYAERRLANGGKLPASVA
                     PLYALGWLLVYRPLKKRLGLNRCRIAVSGAAPIAPGVLHFFHAIGLPVREGWGQTESC
                     GVGTISPGDDIRIGTIGKAFEGVEIRIADDGEILMRGPHVFAGYYRNPKATAETVDGD
                     GWLHTGDVGRFDDNGHLHIVDRMKDIIITAGGKNISPSEIENKLKQSPYVKEAIVVGD
                     RRPYCVALIGIELDTVGDWATRQRIPFTTYGDLAAKQEVVRLIEEHVHRINKELAPVE
                     QIKTFRLLPKELDHEDGELTATQKVRRGVLAERFESLVEEMYKK"
BASE COUNT         2198 a         4581 c         4780 g         2100 t
ORIGIN      
        1 cgccgaacgg atcgaggcga tggcgcggcg catcgccagg gtcggtccgt gcaccagctt
       61 cgcggcgatg cgccgcgcca tcgcctcgat ccgttcggcg tgctgcggcg caaggtccat
      121 gccgcccagc gcgcgagcgg cttcggcgcg ggcgacgcgc tcggccttgg cccgcagcgc
      181 cgcgatgtcg cggcggcggc cgctgggatc caggcggcgg atcagctcgg cggtcgcggc
      241 gcgcacggct gtttcggcgt cggacacgac ggccgaggcc gcgaccggtg acaggtcgtc
      301 cagcgtgcgc acgtgcacgt gcgggtggtc gcggacggcg acggcgcgcg gcacggacac
      361 gtccacgatc gccagcggcg cggttcgttc gacgatgtgg gtgggtcgca ccagcacgcc
      421 gcccgcgacc gcgacgaccg cgaactccga cgtcgccagc gcggcgggaa gcgcgtccat
      481 cggtcgcggg tcgccatcga cgcgcgcggc cagcgcctgc gtcgtggccg gggtccggcc
      541 gaccacgatc agccgggcgc ctgcggtgcg cagcgcgatc gcggcgtcgg ccgccgcttc
      601 cccggagccg acgattgcac ccgggcgtcc tcccaggccg tcgagccgcg ccgtcaggtc
      661 gccggcgacc agcgcgccga tcgatgcggt ggccgtgccc ggcagaatgc gcgagcgggt
      721 cagccgtccg acacggatcg cctccgagaa gatggacccc agcaccggac cggccgcgcc
      781 gtacgacgcg gcggtctgga acgcgcgccg gacctgtccg aggatctgat gctcgccgac
      841 gaccagcgag tcgaggccgc tcgcgacacg cagcagatgc tcgatcgctt cgccgccgcg
      901 ccggaagcgc agctccgcct gatcgccggg gcggatcgcg ggaagcaggc gtgcgagcac
      961 gtgagcggcg tccgcgtcgg cgatgcccgc ggtcgttgcg tacagttcgg tgcggtggca
     1021 cgtgctcagc acgacgagct cgccgatgcc gggccgcggc gtggccagca cgcgatcgag
     1081 gccggccgtg tccagcgcga tccgctcacg cagctctcgg gacgcgtccc ttcctgcccc
     1141 cagcgaaacg atcatgcctc cgccccccgt ccggctcacg acgcgacgcg ccaggaagct
     1201 tcctcggcga gcaccgcgtc ggcgatggcc cggcacagtc tcgggtcggc gttcggtgac
     1261 gggatgcgcc gaagctccac gccgagccgt ccggcggcct cgaccgcctc gatgtcgatg
     1321 tcgaacagaa tctccaggtg atcggccacg aagccgatcg gcgcgacggc cgcgcggcgg
     1381 ccaccgtccc cggcgaagat cgacagggcg tcgagcaggt cggggccgag ccacggctcg
     1441 cccgtctggc tcgcgctctg gaacgcgaag gaccaatggc ggatgcccgc gcgctgcgcg
     1501 atgcgctccg ccgaggccag cagctggtcg cgataggggt cgccggcggc caggatccgc
     1561 tccgggaggg agtgcgcggt gaagaaaacg tgcgtggcgg gatcggcggg atcccagccg
     1621 tgcatggtct cgcggaggtt gtccgcgacg agcgcgctgt agcccggctg ctcccaccac
     1681 gactcgatca tgcggaaggt gaagcgcgcg ccggtctcct tcatcgcgcg ctcgacccgg
     1741 ccggcgtagc cgccgatgct catcgccgag tagtgcggcg cggccgcgac gccgatgacg
     1801 cgccgcacac tcgacgcatc gatctcctcg acggcctggc ggatccaggg ctcccaatgc
     1861 ttcatgccca cgaacacgcg gtagtcgccg tcgtcggaga gcgcgcgctc gatgcccgcc
     1921 gcctgcgcga gcgtcacgtc gagcagcggc gaaggaacgc cgacggcgga gtagcggcgg
     1981 atcagatcct ccagcagccc gtcgcggatc gggcggtcgc cgcggatatc ccgcagatag
     2041 ggctcgatct cttcgatcga gttcgggctg ccgtaggcca tgagcagaac accggtcgtc
     2101 atgcggtctc ctcgtgtacg aagtcgacga gtcgtttgat gttgtccagc ggcgagtcgg
     2161 gaagcacgcc gtggccgagg ttgaagacgt gaccggggcg gccgtccgcg cggcgaagca
     2221 cgtcgcgggc gcgcgtttcg atcagctcgg gcgggccgag cagcaccgcc ggatccaggt
     2281 tgccttgaat gccgagatcg ccgatcgcgc gccaggcgtc gtcgagagga atgcgccagt
     2341 cgaccccgac gacgtcgggt ccggcttcgg ccatcacgtc gagcatgtgc gccgttcccg
     2401 tgccgaagtg gatacgtggc acgccgctcc cgtcgagcgc ggcgaagatc cgtttcgaat
     2461 acggcaggac gtaggtgcgc gtgtcgtgcg cggacagggc gccgatccac gagtcgaaca
     2521 gctggacggc ttggacgccc gcggtgatct gcgctcgcag gtacgcgatc accagcgtgg
     2581 tgagccgctc catcagcgcg gcccacacct cgggggcgcc gaacatgatc gccttcgtct
     2641 tcgcgtattc gcgcgacggg cgtccttcga tcaggtagct cgcgagcgtg aagggggcgc
     2701 cggagaagcc gatgagcggc gcgcggcctt ccagctcgcg ccgcaggatg cggatcgcct
     2761 cgaacacggt cgggacggac tcttcgatcg ggatgcgggc catcgcatcc acctgtccgc
     2821 ggcggcggat gggctcgtcc acgacaggac cgatcgactc gacgatctcg aatcgcacgc
     2881 cgagcgatgc gagcgggagc atgatgtcgg caaacatcac cgccgcgtcc agatccatct
     2941 cgcgcatcgg cagcatcgtg acctcggcca tcaacgccgg atcgccgatc acctcgagga
     3001 agccgtacgt ttcgcgcagg gcgcggtagg cgggaatcgt gcgcccggct tggcgcatca
     3061 gccagatcgg cgtgtagggg acgggctcgc cgcggcatgc gcgaaggaac ggcgactgcg
     3121 acgacgaaag cgtttcgcga gcgctgatgg cggccacggc gcggaacata ccacggcgca
     3181 atagcacgtg ttgcttgacg acgtgtcgtc atctcgatgg cgacagccgg tcgtaatggt
     3241 cgcgacgtgc ggtcgcgaaa cgggactggt tcccggcggt ctgcggtagt ctggtgcttg
     3301 ctatggcacg caccggcgga gaggaacgca agcgcagcct ggttgctcat gcggtccggc
     3361 tgttcggatc gctcggctat gacggcacgt cgttggactc ggtcgccgaa gcggccggtg
     3421 tgcgcaagca gacccttctg tactacttcc cgacgaaaga ggcgctgttc gaagcgtgcg
     3481 tcgaacaagt gggtgcgcgt gccgcccagg cgctggaggc cgcgctcgaa ggaccggagg
     3541 aaggattcgg ccgcgccgag gccgtgatcc ggaccatctt ccgattgacg gaagagtggc
     3601 cggagtttcc gaccttcatt cgcgaggcct cccgcaatgg cccggctgcg gtcggccgga
     3661 tcgccggcac gctcgagccg ctgcgaaagc gggcgctcgt ctttctcgaa aaggagatgg
     3721 ccgagggcac cttccgccgg caggatccga cgctgctgct gttcacgctc tacaccgccg
     3781 tcgtcggctc ggtcaccgaa gccggcgtgc tgcgcgcggt cggcggggga gcggtcggcc
     3841 gcgacgcgtt gaagcggcgc gaggaagagc tgatcggatt cctccgggtc gcgctcaccg
     3901 gcaagaacta agcatcggtc cacctcttga cggaatcagt cgagccctga tatatctact
     3961 ggcgatatag cgacgctcga tagagaggac ggggatgggc gatctcgaag gcccgctgcc
     4021 ggcggcgacc tttcacatcc tgctcgcgct taccgatggc ccgaagcacg gctacgcgat
     4081 catgcagcgg gtcgaggatc tcagcgacgg ctcggtgcgc atgggaccgg gcacgctcta
     4141 cggcaccgtc aagcgcatgg tgaccgacgg cctgatcgag gaggtggccg gcccggacgg
     4201 ggaagacgac cgccgccgca cctaccggct gaccgcggcc gggcagacct cgtgcgcggc
     4261 cgaggtccgc cggttgtcca agcttttgtc ggacgccgcg gtgaagcgat ggtcgcgcgg
     4321 tctgagcccc ggcaccgcat gagcgcgcct cggtggtatc gcgcggtgct cctgctgtac
     4381 ccgcggacct ttcgccgtga gtacggcgac gagatggtgg ccgtgctcga agacatgatg
     4441 gccggcggag cgcgcggccc gggactggtg acgaggatcg tgcgggacgt ggtggtctcg
     4501 gtgccgcgga tccgtcgaga ggagacgacg atggcaacga aagcaatcat cgcaggcgtg
     4561 ctggtgctga ccgtggggct ggcagtcgcc ggaacggggt cggtcgcgct gggcgggatc
     4621 gtgtcgggga ccgtcctggc cgtggtgctc ggcggcgcga cgctgcttgc gcggcggggc
     4681 tcggcatcga ccgaggcggc ctaccgggcc gcgcggccgg tctggcccgt cgccgcggcg
     4741 ttcgtcggtg cctacgcgct gttcacggcg gttcagcagt acgtgagcga gccgacggcg
     4801 gcaagccgct cccagcttct gttcgggctg atcgcgcccg cgctggtggc cggcggcctg
     4861 tgggcccgcg cggcgggacg gcgatgggcg gcgatcccga tcgtggccgg atgcgcgccg
     4921 ctgctcgtcg tgttctggtt cgttccggtg ccggtcgccg cgctcgtcgc ggtcatcggc
     4981 tcgatcgccg agtcgatgcg ggcatcgagc cgcccccggc cggccgtcta ggacgcggcg
     5041 ggaatcagcc gggcggaagg ttcatgagct tctgctcgtc gccctgatca cccttggtcc
     5101 tcaggtaggg ctcgtgcccg cagagggggc acttgccttc gacgacatcg tgctcggcgt
     5161 cgccgtcgac gatatagaag cggctgccga gcgcggtgag ttggatgacc cgctcggggg
     5221 agagcatgat cggctcggcc gccaggtgca acgagtgata gccgacgagc tcaacgtgat
     5281 cgtgcttggc gtcggcctgc tcgagccgtg acgcgacgac cttcagcgat gccaccgaac
     5341 ctcccctcga accgatccgt gcggagcata ggttaccggc ttccgaggcg cacgcgaatc
     5401 ggttcggaac ccttgcgcgg cggcccggcc gcaccgacaa tcagtccacg gagaaccggg
     5461 ggagggcggg ggccatggga gtcgatgacc gtcgccgagc gggcctggac cgcaacgacc
     5521 gagcaggagc ggttgggcct tctcgtcgat gttcttggac ccgaccgggc ggccgagatg
     5581 acgtcctcgg acttcgcgcg catcgtcgac ttcggccgcg tcaaccgcca ggcggggcgg
     5641 cgggcgaccg tgctggcctg gatcgcattc gcgatcgtga ccgcggtggg cattcggcgc
     5701 acgcgcagtg aacgcgcgcg atcccttcgc ctccgtttca ccctgaccgg cgcgacgcct
     5761 ctcgaaggtt catcgcggcc tggatcagcc cgaggtggct gaaggcctgc gggaagttgc
     5821 cgagcagatc gccggtctcg ggatcgacct cttcggagag caggccgaga tcgttggcgt
     5881 ggccgagcat gttctcgaac agctcggtgg cgcgctcgac ctcgccggcc ttcgcgagcg
     5941 cgccgaccat ccagaacgta cagatgacga acgcgccctc gccgccgggc aggccgtcgt
     6001 cggtcaggta ccgtcgcacc aggcagtcct ccatcagccg ctcctcgacc cgccggatcg
     6061 tggcgagcac ccgcgggtcg tcgccgggca ggaagccggt cagtggaatg agcagcagcg
     6121 acgcgtcggg ctcgtcggtc tcggggctct ggcgaaaggc gttcgtggct ggatcgacgc
     6181 cgttggtttc gatccaatcg cggatctcct cgcgcagcgc ttcccaccgt tcgaggtcgg
     6241 cggcaaagcc ggagtctttc gcgaggcgga tgccgcggtc caccgccagc cacgcgaaca
     6301 gcttcgaggc gacgagctcg cgcggctcgc cgcgcagctc ccagatgccc cggtcgggct
     6361 cgcgccagca gtccgccacg accgacacga cgctgcacag gaactgccag aacggctcgt
     6421 cgatctcgcc gccgtttcga tggtagagcc agaccgtgtc catgagctcg ccgtacatgt
     6481 cgagctggaa ctggtcgacc gccccgttgc cgatgcgaac gggcgcggag ccgcggtatc
     6541 cctccaggtg gccgagctcg atctcgggca ccatgcgctc cccgccgacg ccgtagagca
     6601 cctgaaggtt ctcggcggtc cctgccgcgg tgcgctgcag ccactgcatg aatgcatgcg
     6661 cctcgtcgat gtagcccagg cggaacagcg cgcgcaggtg gagcgccgcg tcccgcagcc
     6721 aggtgtagcg gtagtcccag ttgcgcacgc cgccgatctg ctcgggaagc gaggtcgtcg
     6781 gcgcggcgac gatcgcgccg gtcgggtcgt aggtcagcgc cttcagcacg agcgcgcttc
     6841 gcaggatctg atcgcgccac ggcccgtcgt accggcagcg gcccgaccag gcccgccagt
     6901 attcgagcgt ctcccggtac cgccggccga tgacgtcgtg ctcgacgcga atgccatcga
     6961 gatcgggagg atcgacgtgg gtgagcacca cgtatcgctc gtcgccggcc cgaagcgtgg
     7021 tgcggatgcg ctcgtcgctg atgtcggtct gatcgaggcc gatgtcggtc tgcagcacca
     7081 gcgcgtcggc gcccccgaag acgatcgcgc gatccggctc acgcaccagg acgcgcggcc
     7141 gggtcaggcc gtactcgaag cggggcgaga agtgcttctc gatctccacg cttcccgact
     7201 cgcatcggat cagccggatc agctgctcgt agggatgggt gtgctccggg ccggatctgt
     7261 gcggtgactc gaccgtgagg aagtccgtga gcacgatcac gccgccgtcg gtctcgaatc
     7321 gcgtctcgag cacgttggtg ccctcgatgt atctgcgcgt caccgtgaag ggatcggtcg
     7381 gcgcgatgcg gaagtgcccg cccttgacgc gatccaggat cgcggcgaag accggccggg
     7441 agtcgaaccg gcggaagcag caccagtcga tcgacccctc gcgcgagacc agcgccgagg
     7501 agtgacagtc gccgagcagc gcgtacgacg cgatcggcgg atactcgttg agcgacatga
     7561 gcgatccggg ctcaccttac cggtcgcacc gcacccgcgc tggggcgcgg tgtcaggcgg
     7621 tgcccggtgc ggagcgacgc gactccagcc ggcgcacgcg ggcggcgtcc tgtaggcgcg
     7681 ccgttctttt ggcccgggcg gtctcgatga cgaacgcgac gatgagcggc gtcgtctcgg
     7741 cgatgttgta gaggaaatgc aggtccgcac gcttcagcgg aaggccgccg ccgatcacgc
     7801 cgccggcagc cagcaagccg gccgacccgg cgtcgcccgt ggacagatac accgacatga
     7861 tccaggtgtg ctcggcctga tgccagatcg cgagtgcgaa cgccgcgagc agcagccggc
     7921 tgtgccgata ggcggcgagc agcgaggcgg ttgcgagcag gatgaaggtg ttccagccga
     7981 agtggaccca ctcgacgtcg agcgggccga agatgccacg cgagtgcgcc ggcggcagat
     8041 gcagcaggtg cagctgaacc atctgcgtga cgtgctcgac gagatggccg ccctggctga
     8101 ccgcgaccac gtagaagagc gcgagccatg ggttgcggcg ggcgacctcg gcgacgtcgc
     8161 gcaggtagag cggaatccac gggccgagcg tgattagcgc gaccgaccac aacggccatg
     8221 cgagcgccga ggcggcgagg ccggccgaga tcgacgcgcc gcccgcgatg acgaacacgc
     8281 gcagcggaag ctcggcggag gcggggacca ggcgaagggg aggaggctgg gcgagccgct
     8341 gctcgcgcgg cggggacgcg ggtgcgacgg cgacgggtct gctcacgaaa cggcctcctc
     8401 cctcgcgagt ggaaacgagg ccggggccgc cggcgttcta ccgagcggcc gcgcgcataa
     8461 cggaaaaggc ggaaggcccg gcgaatgccg ggccttccgt gatcgctacg atcagtcgca
     8521 gaaggggaag tccttcacga tcgggtcgtc gaccgcggcg agccgcttga gcccggtcgg
     8581 cgccgaagcg tccaccgagt agatcgcggt gcccgtggcc ggaaccttgt cctcgcactt
     8641 ggtgccgtac gagatcgggt tcagcaagcc gcccatgtcc acgtccgaca ggctttcgaa
     8701 ggccttgagc acgccctcgc gggtcaggtc gcccatggtg atcgccttct cgaggatctg
     8761 gtggacgatc ttggcctgcg tgtagccgaa ctggaagtag gtgtcggggg tttgtccgcc
     8821 gccgtacttg gcgaggttgt ccaccatctc cttcatgccg tcacagcccg cggcggtcga
     8881 tccccagtcg caggccgcgt cgccggtcgc ccagaagtac gccttcaggt agtcggcgag
     8941 cgggctcttg gccagcgcgc cgatccacgc gggcgactgg ccgatccact gcggcgtgta
     9001 tttcagctgc gccgccgcac cgaggatgcc gccggtggcg ctgggcacgg tcaccaggaa
     9061 cacgtgatcg gccccggcgt tcttcatcgc cgtcacctgc gagacgaact cggtatcggt
     9121 cggcttgtag gttgcacgcg cgacgatctg gaagttcagc ttgccggccg catacacgac
     9181 gcctttcagt ccgtccgcgc cgtagtcgtc gtcctggtag atgaccccga ccttcggtgt
     9241 cttcttctgc tcgttgacga cataggcgaa tgcgttgatc gcctgcaccg gatacggggc
     9301 gccggccggg atcacgtact tctctttcag ccagtcgctg gacagtgtcg ccgcgccggt
     9361 gaggatcttg tcggcacgca cctgctccag gatcgccttg gtcgtgggcg ttcccagcga
     9421 ctgcgcgatc agcagcacct tgtccttcag cttgccgtac tgctcggcat gcgtctgcgg
     9481 cacgtattgc gtgtcgagcg tctccagctc gaccttgaac ttcttgtcgg caggcaggtt
     9541 cttgccgacg ccgccgagca cctcgttcac gtactggaag tagacctcgt ggcctttggt
     9601 cagcggcttg ccgatcacgg cgaccggtcc ggtcagcggc gacagctcgc ccaaggtgat
     9661 ggtctgcccc gtcacgccga caccggtctt gatctcgccg ctggtcccgt tgcctccgtc
     9721 gcccgaggac cggcacgcag ttgcgaccag tgcaagcacg gcgacggcta cgattgtccg
     9781 aacacctcga cgcatgtgtc ctcccctgat gcggttgctc tccgagtgct ccattgctcc
     9841 tcctcccgcc cggccggtca atagctgaac ggccatgtct tgaaataact cttcgcgcgt
     9901 cgccacaaac cggcgcaccc gtacggctcc gctactagga atacgatgat cagggctccg
     9961 aagatgatct gattgagctc gaacacggta attccttccg cggtggcggt cgtcgccacg
    10021 aacggcagcc tgtccgagaa ctcttcgacc agcctgggaa ccagcgcgat gaagaacgcg
    10081 ccgaggatcg ctccgaacac cgtaccgatg ccgccgatga tgatcatcgc cacatactgg
    10141 atcgacagga acagatcgaa ctggctcggg gcggcgaagc gaaggtacga gcccagcatc
    10201 gcgccgccga tggccgcgta ggcgctggag atcacgaacg ccgcgacctt gtagcggaac
    10261 aggttcacgc cgatgatcga tgcggcgatc tcgcggtcgc ggatcgcctg gaacgcgcgg
    10321 cccgtccgcg accggacgat gttctttgcg atcagcgcca tcagcaccag catggggatc
    10381 gccagcagat agaagctttg atcgcgcgag aacgttccga tcgcgttgaa cgagataccg
    10441 ccgacctcgg gcgcaggaat cgaacgccct tgcggcccgc cggtcacgtc gcgcagattc
    10501 aagaacagat gctcgcccaa aaagacgagc cccagcgtga cgatggccag gtacagccct
    10561 ttcaggcgaa gtgcgaacgg cccgacgatc agcccgacga ttccgccgag gatgcccgcg
    10621 gccggcaacc agaccacgaa cggcaagccg tgattgccgc ccagatatcc ggccgtgtat
    10681 gcacccagtc cgatgaagaa cgcgtgcccc agcgagacct gaccggtgaa gccggtcagc
    10741 aggttgagcc cgatcgcgcc gatagatgcg atcgccgcga agttgatcac cgccagccag
    10801 aagtcggtga gcgtgaaggg aagcacgacc acgacgacgg ccagcaaggc catcccgatc
    10861 ttggcgcctc gggtgggaaa gatcgaaacg ctttgggcgt acgaggtgtg cagcgtttcg
    10921 ggccggcgcg acgtgagcgc gttcatacgc gttcgacctc ccgggtaccg aacaggccgt
    10981 aggggcggat catcagcacg accaccatca ccgcgtaggg ggcgaccacg tggaagctat
    11041 tgcccagcca cgacggctgg taggcctggg tgagcacctc gatcagtccg atcgccaaac
    11101 cgccgatcac cgcaccgccg ggcgagtcga gcccgcccag gatcgccgcg ggaaacgcgc
    11161 gcagcgcaac gaacccgacc gtcggagaca gcgacttcgg aaacgtggcg atgaacacgc
    11221 cggcgaccgt cgccgtcgcg cccgcgatcg cccatgagat cgcggtcatg cgccggaccg
    11281 agatccccag cgacagcgcg gcttcctgat cgaacgcggc ggcgcgcatc gcgacgcccg
    11341 acttgctcga gcggaagaag atgaaaaagc ccaggagcac caggccggtc gccaccaaag
    11401 tccagatgct ggccgcggtg acgctcagcg cgccgacgtc gtagacggtc gcgccgaacg
    11461 gatcatcgat gtggcggtcc gcgaaacccc agaccgaggt cacgatctgc cggatgacga
    11521 tcgcgaggcc cagcgtgatc atcacgatgg cgaacacggg ccggccgacc atgcggcgca
    11581 gcacgacgcg ctcgattccc aggccgacgc cggccataat gacgatcgcc agcaagagcg
    11641 cgaccgcgaa cggcaagccg atctggttgc gccacagaaa gacgaacgag cccccgagca
    11701 gcaccagctc gccgtgcgca aagttgatca cctcgctgga cttgtagacg atcacgaagc
    11761 ccagcgcgat cagcgcgtag acggcgccca acaccacgcc ctgataaacg aactgaagga
    11821 actccgtcac ttcttgtaca tctcctcgac caaggactcg aagcgttcgg ccagcacgcc
    11881 gcgccggacc ttctgcgtgg ccgtcagctc gccgtcttcg tggtcgagct ctttgggaag
    11941 cagccggaag gtcttgatct gctcgacggg ggcaagctcc ttgttgatgc gatgcacgtg
    12001 ctcttcgatc agccgcacca cttcctgctt ggcggccaga tcgccgtagg tcgtaaacgg
    12061 aatccgctgc cgcgtcgccc agtcgccgac cgtgtccagc tcgatgccga tcaacgccac
    12121 gcagtacggg cgccggtcgc cgacgacgat cgcttccttg acgtacggcg actgcttcag
    12181 cttgttctcg atctcggacg gagagatgtt cttgccgccg gcggtgatga tgatgtcttt
    12241 catgcggtcc acgatgtgca ggtggccgtt gtcgtcgaag cggccgacgt cgccggtgtg
    12301 cagccatccg tcaccgtcta ccgtctcggc ggtggctttc gggttgcggt agtagccggc
    12361 gaacacgtgc gggccgcgca tcaggatttc gccgtcgtcg gcgatgcgga tctcgacgcc
    12421 ttcgaacgct ttgccgatcg tcccgatgcg gatgtcgtcg ccggggctga tcgtgccgac
    12481 gccacacgac tcggtctgtc cccacccttc gcggaccggc agtccgatcg cgtggaagaa
    12541 gtgcagcacg ccgggcgcga tcggcgcggc gccgctgacc gcgatccggc agcggttcaa
    12601 cccgagccgc ttcttcagcg gccggtagac cagcagccag cccagcgcat atagcggggc
    12661 aaccgacgcc ggcagctttc cgccgttggc caggcggcgt tcggcgtacg tctggccgcg
    12721 tttgagccag aaccggaaca gtgcgcgctt gaccggcgag gcgtcgcgca tgcggatctc
    12781 gatgccggcg gccatcttct cccagatgcg cggcacgccc aggaaaattg tcggttcgac
    12841 ttcacgcagg ttctcgggca ccgtgtcgat cgattcggcg aacgaggcgc tgacgccgaa
    12901 cgcgatcggg aagtagatgg tgaaaatctt ttccgcgacg tggttgagcg gcagatagga
    12961 cagcacccgg tcgccgtcgg tgatctcgtc ccaggcggac ttcgtcagcg cgatcgacgc
    13021 tgccaggttc gcctgcgaca gcatcgcgcc cttgggcggg ccggtcgtac ccgaggtgta
    13081 gacgatcatc gcgatctcgt cgggcgtgcg ctcggacacc aggcgttcga agtgcgacgg
    13141 atcggcggcg tgcatctgcg cgcccgccga cagcacgtcc tcgaacggca tcagccgcgg
    13201 atcgtcgtag ccgcgcaggc cgcgccggtc gatcacaacg atgcgctcca gcgacggcag
    13261 cttcacctcg aggatcttgt ccacctgctc ctggttttcg gccacgacca cccgggcgcc
    13321 gctgtgctcg aggacgtagc cgacttcggc ggcggggctg gtcgggtaga cgccgacgct
    13381 ggtcgccccg gcggtgacgg cgcccagctc ggcgaagacc cactcgggcc ggtcgtcgga
    13441 aaggatcgcg acgcggtcac ccggttggat gcccagccac accatgccca gcgcgatctc
    13501 gcgcacgcgc gcctcgtaat cggaccagct gtgctcgcgc cagatgccga aggctttctc
    13561 gcgcaacgcc acgttgccgg gggcgaggcg ggcgcgcccg acgaacagcg ccggcagcgt
    13621 cgccggctgc tgcgcgggct gccggcgctg ttcgtcggg
//
LOCUS       DATLFO010000126        10931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_92926, whole genome shotgun sequence.
ACCESSION   DATLFO010000126 DATLFO010000000
VERSION     DATLFO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92926"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VM841_04630"
     CDS             complement(<1..336)
                     /locus_tag="VM841_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89504.1"
                     /translation="MSPPSPVVAPPLASRDEEPAPISTSISPDLIAAAVAEVERSEEP
                     HPAPADLPAAPPLFTAPKPLGDASAPAPLIPPRPLGAPAERTMPAPLHQPPAPLHQPP
                     APLHQPPAPL"
     gene            651..1034
                     /locus_tag="VM841_04635"
     CDS             651..1034
                     /locus_tag="VM841_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89505.1"
                     /translation="MFRRRPPHPVLEPFLHMAAALDDAQRALLAAIPTARDDGIPLAE
                     AIVAFERGLAETEARMPAWRTPATAAMHDECAAAIRAARVECETLRLEPETLSFEALN
                     ARIGDVLHPLEVFADTERALRRGGS"
     gene            complement(1031..3616)
                     /gene="clpB"
                     /locus_tag="VM841_04640"
     CDS             complement(1031..3616)
                     /gene="clpB"
                     /locus_tag="VM841_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HVL89506.1"
                     /translation="MDFERFTLKAQEAVAGAQRLAASRNHQVVEPEHLLDGLLADPEG
                     IVFPAVQRIGASPRTLRDRNGEILDRIPRVFVEGGEAKVAGSPALARVLEHAVEETRN
                     LKDDYTSTEHLLLALTEVAGPVASLLKSAGVTRDSVLEALQQVRGSQRVTSQTPEGTF
                     QSLEKYGRDLTAVARSGKLDPVIGRDDEIRRVIQVLARRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIAAGDVPEALKNRKVISLDLAGMVAGTKFRGEFEERLKAVLKEIAESEGGVI
                     TFIDELHTIVGAGAAEGSMDAGNMIKPMLARGELRMIGATTLDEYRKHIEKDAALERR
                     FQTVFVGEPSVEDAISILRGLKERYEVHHGVRITDAALVAAAMLSHRHVSGRFLPDKA
                     IDLVDEAASRLRIEIDSMPTELDDLTRQIMRLEIERAALKKETDEASKERLERIDAEL
                     AELRERETSMTAHWSQEKEAVEAIRGLKTKIEDVRTSAELAERDGDLERAAELRYGTL
                     VSLERDLASATETLASLQTDRQYLKEEVDEEDVAQVVSAWTGIPVAKLMEGEVAKLVH
                     MEENLHGRVVGQDEAIAEISNAIRRSRSGIADPNRPIGSFVFLGPTGVGKTELARALA
                     EFLFDDERALVRIDMSEYQERHAVSRLVGAPPGYVGYEEGGQLTESVRRRPYSVILLD
                     EIEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNSIVIMTSNLGSAAIMDPALPDERM
                     RETVMAEMRASFRPEFINRVDDILIFHRLSPDDLRRIVDIQLTRLERRLAERKIEISL
                     TDKAREWLAGRGFDPAYGARPLKRLIRKQIEDALAMRLLQGEFAEGDRVEIDAGDEAL
                     AFSKA"
     gene            complement(3632..4054)
                     /locus_tag="VM841_04645"
     CDS             complement(3632..4054)
                     /locus_tag="VM841_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL89507.1"
                     /translation="MKTVDFPTPKTAKRLTDDRDRALYVISVAAELADVHPQTLRMYE
                     RKGLISPQRTAGNNRRYSQRDIERVRLIQSMTQEHGMNLAAVEVVISLQRELDEAKRR
                     LDQLEDEVDRARREQERAASSGGALVLRRDIQDLFGAG"
     gene            complement(4051..5148)
                     /gene="dnaJ"
                     /locus_tag="VM841_04650"
     CDS             complement(4051..5148)
                     /gene="dnaJ"
                     /locus_tag="VM841_04650"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVL89508.1"
                     /translation="MNANWLDKDFYEILGVAENASAEDIKRAYRKLARKHHPDRNPGD
                     KAAEETMKKISEANDVLSDPKKRSEYDQMRRMARSGFGGVPGGGGGNANFNVGDLGDL
                     GGIFGDLFGGGRRRRGPARGPDLETGARITFDQAMAGETVAVRVQRDTPCDTCGGSGA
                     APGTAPSACRSCGGSGMVGENQGLFSFQRPCGACGGAGRVIDTPCPACAGDGSVRRND
                     EVKVRIPAGIADGARIRARGRGGAGPRGGQPGDLFVVVRVAPHPVFGRSGADLTLDVP
                     VGYAEAALGGEIKIPTLEGPVTLKIPAGTQPGRTFRVKGRGAPKPKGGRGDLLATVRV
                     VVPENLSDDERALLEQLRARQNGALRAHLGV"
     gene            complement(5171..5830)
                     /gene="grpE"
                     /locus_tag="VM841_04655"
     CDS             complement(5171..5830)
                     /gene="grpE"
                     /locus_tag="VM841_04655"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HVL89509.1"
                     /translation="MTDEARTPEEERRTQDGEQPAAPEAASGTASQASDERSRAYSES
                     PPMREDASEAPEADEGTGEGTDEGMDREIGRLRDEAQAHLEDARRIKAEFENYKKRML
                     REQTTIVERASGGLIERLLPVLDSFRLALVAADRTKNDREFLRGVELVYGELLEVLKK
                     EGLEEIDAEGKPFDPEVHEAVMQVSSPDVPDGHVAEVMRTGYTVRGRVIRPAMVSVGK
                     S"
     gene            complement(5827..6348)
                     /locus_tag="VM841_04660"
     CDS             complement(5827..6348)
                     /locus_tag="VM841_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVL89510.1"
                     /translation="MTIAFVRRIPAAQGRRTSGGNPEEVDTMTNLSKWDPFRDLLGIQ
                     TEMTRLFDRAYGDNGTGARMWAPPVDIAETPDGYRVVIELPGFGPDQVDVTVTDGALT
                     VKGERKFYTDVAEESFHRVERRFGAFQRTVALPTQVQADRIEASFDNGLLTIDVPKAE
                     AVKPRRIEIKAGR"
     gene            complement(6345..8195)
                     /gene="dnaK"
                     /locus_tag="VM841_04665"
     CDS             complement(6345..8195)
                     /gene="dnaK"
                     /locus_tag="VM841_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HVL89511.1"
                     /translation="MAKVVGIDLGTTNSVVAVLEGGDPTVIANAEGARTTPSVVAFSK
                     TGEILVGEVAKRQAITNPDRTVRSIKREMGRKDWTLDIDGKKWTPQEISAQVLMKLKR
                     DAESYLGDKVTQAVITVPAYFDDAQRQATKEAGQVAGLEVLRIINEPTAAALAYGLDK
                     NTTDHTVLVFDLGGGTFDVSLLEIGDGVFEVKATHGDTQLGGDDWDQRVIDWMVAQFK
                     NNHGVDLGNDRMALQRLKEAAEKAKIELSQTTEATINLPFITATADGPLHMELKLTRT
                     EFERMTADLVERCRVPFNLAIKDAGMDVKDIDHVILVGGSSRMPMIQNLVKELTGGKD
                     PHKGVNPDEVVAVGAAIQAGVLKGEVKDVLLLDVTPLTLGVETKGGIFTKLIERNTTI
                     PTRKSDVFTTAEDGQTTVEIHVLQGEGEMATSPAVRSLGKFQLVGIAPAPRGMPQIEV
                     AFDIDANGIVQVSAKDTATGKEQAMTITGGTALPKDEIDRMMREAEASADDEKKRRAE
                     AEARNQADNLVYQTEKMLGEHGDKLPAADKDEIQNAIDETKEALKGSDAQAINDAAGR
                     LSTAAQKIGQAMYASQQAAGAAGAEQGQAANEPPQGDVVDAEIVDEGEGA"
     gene            complement(8371..9147)
                     /locus_tag="VM841_04670"
     CDS             complement(8371..9147)
                     /locus_tag="VM841_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL89512.1"
                     /translation="MGERVYSVVIAGALGMIKSMRWPVTVVGGEHVPASGPAILASNH
                     IGYLDFVFGGYAAREQGRRVRFLAKKEIFDKGGVGWFMRQMGHIPVDRHGAADASVRA
                     AVTALQAGEVIGMFPEATISPSFVPRKGKTGTVRAARETGAPIIPVAVWGTQRILTKW
                     RPRNFKRNIPIVVVVGEPLEMIEGEDAEAATTRLMARITELLEHAQRIYPAKPRGDAD
                     RWWLPAHLGGAAPTFEEAERRLAEEQAARKARRASEGPPR"
     gene            complement(9232..9690)
                     /locus_tag="VM841_04675"
     CDS             complement(9232..9690)
                     /locus_tag="VM841_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HVL89513.1"
                     /translation="MRRSARALFVGLILLLAACGGSNGDGEVRVAIIEGARRAVVTAE
                     VASTPDQRATGLMGRREIPEARGMLFVFPSPTRGGFWMKNTLVPLRIFFIRAGTVVDV
                     REMQPCTADPCPLTIPDEPYDRALEVPLTALAGFGPGARVEVEGRVPQPV"
     gene            9737..10261
                     /locus_tag="VM841_04680"
     CDS             9737..10261
                     /locus_tag="VM841_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVL89514.1"
                     /translation="MHVAVTAVGRDRPGIAAAVSKVLFEHGANIEDSRMAILGGHFSM
                     MLIVATDADAQTLERDLAAATASLDLTVAVRAVEDAASAASEGAAFTLSVYGADHPGI
                     VYRVTEALAERSINVTDLATRVIGDDPPVYVMIMEVTVPAGLNAAALDDGLKHLGADL
                     GVDVSLRPAEADTL"
     gene            10264..10764
                     /gene="def"
                     /locus_tag="VM841_04685"
     CDS             10264..10764
                     /gene="def"
                     /locus_tag="VM841_04685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVL89515.1"
                     /translation="MAVRDVVRYPAAVLKSPAATLDPGPEAEAIAADLIETMRASPAC
                     VGLAAPQIGLGVSAFALDVTGHKKARSCAGLVVMINPEIVAHDGSELAREGCMSLPDF
                     TGNVRRAQWVRVRGLSPAGETVEIEADAFEARAFQHEIDHVNGLLFLDRVVSMTSDVF
                     ERKRYR"
BASE COUNT         1518 a         3853 c         3667 g         1893 t
ORIGIN      
        1 gagcggcgcg ggtggctggt ggagcggcgc gggtggctgg tggagcggcg cgggtggctg
       61 gtggagcggc gcgggcatcg tcctctcggc cggcgcgccc agcggccgcg gcgggatcag
      121 cggagccggc gccgatgcgt caccgagcgg cttcggtgcg gtgaacagcg gcggcgcggc
      181 cggaagatcg gccggagccg ggtggggctc ttcggaacgc tcgacctcgg cgaccgctgc
      241 cgcgatcaga tccggcgaga tcgaggtcga gatcggggcg ggctcctcgt cgcgggaggc
      301 gagcggcggc gccaccaccg gcgagggcgg actcatcgcg gcaggctcga gcgacgagga
      361 aacggacgag gaaacgaagg catcactgcc atcagtcgtg acgggagcgg ggatcgcaga
      421 tcccgcggca aagaccccat cgggcagcac gtgcccgagg aaacacttcc cgttcgggcc
      481 ggcgggaacg tccatcccgc accactcgca ccgaaccatg ccaagcctcc tatcgccgaa
      541 tcgattctcc ccattgagtc ggaccaccat cggtccgtct tgaaggacgt ccacggggaa
      601 cgcacctgtt tggtgcgatt tgttccgccg ccaggactag tctgtggacc atgttccgcc
      661 gccgcccccc acacccggtc ctcgagccgt tccttcacat ggccgcagcg ctcgacgacg
      721 cgcagcgagc gctgctcgct gcgatcccga ccgcacgcga cgacgggatt ccgctcgcgg
      781 aggcgatcgt cgcgttcgaa cggggcctcg cggagaccga ggcgaggatg ccggcgtggc
      841 gcacgcccgc caccgctgcg atgcacgacg agtgcgcggc tgcgatccgg gccgcgcgcg
      901 tcgaatgcga gacgctccgg ttggagccgg aaacgctgag cttcgaggcg ctcaacgccc
      961 gcatcggcga cgtcctgcat ccgctcgagg tgttcgccga caccgagcgg gcgctgcgcc
     1021 gcggcggctc ctaggccttg gagaaggcga gcgcctcgtc gcccgcgtcg atctcgacac
     1081 gatcgccttc ggcgaactcg ccctgcagca gccgcatggc gagtgcatcc tcgatctgct
     1141 tgcggatcag acgcttcagc ggccgggctc cgtaggccgg atcgaagccg cgccccgcca
     1201 gccattcgcg ggccttgtcg gtcagtgaga tctcgatctt gcgctcggcg agccgccttt
     1261 cgagccgcgt cagctggatg tcgacgatcc gccgcaggtc gtcgggcgag agccggtgga
     1321 agatcaggat gtcgtccacc cggttgatga actccggccg gaagctcgcg cgcatctcgg
     1381 ccatcacggt ctcgcgcatc cgctcgtccg gcagcgccgg atccatgatc gcggcgctgc
     1441 cgaggttcga ggtcataatc acgatgctgt tgcggaagtc cacggtgcgg ccctgcccgt
     1501 cggtcaggcg tccgtcgtcg agcacctgca gcaagatgtt gaacacgtcg gggtgagcct
     1561 tctcgatctc gtcgagcaga atcacgctgt agggacggcg ccgcaccgac tcggtcagct
     1621 ggccgccctc ctcgtagcct acgtagcccg gcggcgcgcc gacgagccgc gaaacggcgt
     1681 ggcgctcctg gtactcgctc atgtcgatcc ggacgagcgc acgctcgtcg tcgaacagga
     1741 actcggccag cgcacgcgcc aactcggtct tgcccacacc ggtggggccg aggaagacga
     1801 acgagccgat cggccggttc ggatcggcga tgccgctgcg cgagcgccgg atcgcgttgg
     1861 agatctccgc gatcgcctcg tcctggccga ccacgcggcc gtgcaggttc tcctccatgt
     1921 gaacgagctt ggcgacctct ccctccatca gcttggcgac gggaatgccg gtccatgcgc
     1981 tcacgacctg cgcgacgtcc tcctcgtcga cttcctcctt gaggtactgg cgatcggtct
     2041 gaagcgacgc cagcgtttcg gtggcggacg ccagatcgcg ctcgaggctg acgagcgttc
     2101 cgtatcgcag ctcggcggca cgctcgaggt cgccatcgcg ttccgccagc tcggcgctgg
     2161 tgcgcacgtc ctcgatcttg gtcttgaggc cccgaatcgc ctcgaccgct tccttctcct
     2221 gggaccaatg cgccgtcatg gaggtctcgc gttcgcgaag ctcggcgagt tcggcgtcaa
     2281 tgcgctcgag gcgttccttc gatgcctcgt cggtttcctt cttgagcgcg gcccgctcga
     2341 tctcgagccg catgatctgg cgcgtgagat cgtccagctc ggtcggcatc gagtcgatct
     2401 cgatgcgcag gcgcgatgcg gcctcatcga ccagatcgat cgccttgtcg ggcaggaagc
     2461 ggcccgagac gtgccggtgc gacagcatcg cggccgcgac gagggcggcg tcggtgatgc
     2521 gcacgccgtg atgcacctcg tagcgttcct tgagtccgcg caggatcgag atggcgtctt
     2581 cgacgctcgg ctcgccgacg aagacggttt ggaagcgccg ctccagcgcc gcgtccttct
     2641 cgatgtgctt gcggtactcg tcgagcgtgg tcgcgccgat catgcggagc tcgccgcgcg
     2701 cgagcatcgg cttgatcatg ttgcccgcgt ccatcgagcc ttcggccgcg cccgcgccga
     2761 cgatcgtgtg aagctcgtcg atgaaggtga tcacgccgcc ttcggattcg gcgatctcct
     2821 tgagcacggc cttgagccgc tcctcgaact cgccgcggaa cttcgttccg gcgaccatgc
     2881 cggcgagatc gagtgagatg acctttcggt tcttcagagc ctcgggcacg tcgccggcgg
     2941 cgatgcgctg ggcgagaccc tcgacgatgg ccgtcttgcc cacgcccggc tcgccgatca
     3001 gcaccgggtt gttcttggtg cggcgcgcca gcacctggat gacccgccgg atctcgtcgt
     3061 cgcgtccgat gacgggatcc agcttgccgg accgcgcgac ggcggtcagg tcgcggccgt
     3121 acttttcgag cgactggaac gtgccctcgg gcgtctgcga cgtgacgcgc tgcgatccgc
     3181 gaacctgctg cagcgcttcc aagaccgagt cgcgggtgac gcccgcgctc ttgagcaggg
     3241 atgcaaccgg tccggctact tccgtcagcg cgagcagcag gtgctcggtg gaggtgtagt
     3301 cgtccttcag gttcctcgtc tcttcgaccg cgtgctcgag cacgcgcgca agcgcggggg
     3361 agccggcgac cttggcctcg ccgccctcga cgaatacgcg tgggatgcgg tcgagtatct
     3421 cgccgttgcg gtcgcgcagc gtgcggggcg acgcgccgat ccgctggacg gccgggaaga
     3481 cgattccttc cggatcggcg agcagtccgt cgagcagatg ctcgggctcg acgacctgat
     3541 ggttgcgcga cgcggcgagg cgctgcgcgc ccgccaccgc ctcctgcgcc ttgagcgtga
     3601 atcgttcgaa atccatgggg aagtgccttt cctaaccggc gccgaacaaa tcttggatgt
     3661 cccggcggag caccagcgcg ccgccgctgg atgcggctcg ctcctgctct cggcgcgcgc
     3721 ggtcgacctc gtcctcgagc tggtcgagcc ggcgtttggc ctcgtcgagc tcgcgctgga
     3781 gcgagatcac gacctcgacg gccgccaggt tcatgccgtg ctcctgcgtc atgctttgga
     3841 tcagccgcac gcgttcgatg tcgcgctgcg aatagcgccg gttgtttccg gccgttcgct
     3901 gcgggctgat cagccccttg cgctcgtaca tgcgcaaggt ctgcgggtga acgtcggcaa
     3961 gctcggcggc gaccgagatc acgtacaacg cgcggtcgcg gtcgtcggtc aggcgcttgg
     4021 ccgtcttcgg cgtgggaaag tccaccgtct tcacaccccc agatgcgcgc gaagcgcgcc
     4081 gttctgccgc gcgcgcaact gctcgagcag cgcgcgctca tcgtcggaaa ggttctcggg
     4141 caccacgacc cgaacggtcg cgagcaggtc gccgcggccg cccttgggct tgggcgcgcc
     4201 gcgtcccttg acgcgaaacg tgcggcccgg ctgcgtgccg gccgggattt tcaacgtgac
     4261 cggcccctcc agcgtcggga tcttgatctc tccgcccagc gcggcctcgg catagcccac
     4321 cggcacgtcc aacgtcaggt cggcaccgct tcggccgaac accggatgcg gcgcgacgcg
     4381 aacgacgacg aaaagatcac cgggctggcc gccgcgtggt cccgcgccgc cgcggccgcg
     4441 tgcgcggatg cgcgcgccgt cggcgatgcc ggcggggatc cggaccttca cctcgtcatt
     4501 gcgccgcacc gatccgtcgc cggcgcacgc cgggcacggc gtgtcgatca cccggccggc
     4561 tccgccacac gcgccgcatg gccgctggaa cgagaacagt ccttggttct cgccgaccat
     4621 gccgctgccg ccgcagcttc ggcacgccga cggcgcggtt cccggcgctg ccccgctccc
     4681 gccgcaggtg tcgcagggcg tgtcccgctg cacgcgcacg gccaccgtct cgccggccat
     4741 ggcctgatcg aaggtgatgc gggctccggt ctcgaggtcg ggtccgcgcg cgggcccgcg
     4801 acgtcggcgg ccgccgccga ataggtcgcc gaagatgcct ccgaggtccc cgaggtcgcc
     4861 gacgttgaag ttcgcgttgc caccgccacc gccgggcacg ccaccgaagc cgctgcgagc
     4921 catccggcgc atctggtcgt actcgctgcg cttcttcgga tccgacagca cgtcgttggc
     4981 ctcggagatc ttcttcatcg tctcctcggc ggctttgtca cccgggttgc ggtccgggtg
     5041 atgcttgcgc gcgagctttc gataggcacg cttgatgtct tcggcgctcg cgttctccgc
     5101 cacgccgagg atctcgtaga agtccttgtc gagccagttc gcgttcatcg tctctcgcgc
     5161 gtcgcctgca tcagctcttg ccgacggaca ccatcgccgg ccggatcacg cgtccccgaa
     5221 cggtgtagcc ggtgcgcatc acctcggcga cgtgcccgtc gggaacgtcg ggcgaggaaa
     5281 cctgcatcac ggcctcgtgg acctcgggat cgaagggctt accctcggcg tcgatctcct
     5341 cgagaccctc cttcttaagc acctccagca gctcgccgta aacgagctcg accccgcgca
     5401 ggaactcccg gtcgttcttg gtgcggtcgg ccgcgaccag cgcgagccgg aacgaatcca
     5461 gcaccggcag gagccgctcg atcaatccgc cggaggcgcg ctcgacgatg gtggtctgct
     5521 cgcgcagcat gcgcttcttg tagttctcga actctgcctt gatccggcga gcgtcctcca
     5581 ggtgcgcctg cgcctcgtcg cgaagacggc cgatctcccg atccatgcct tcgtcggtcc
     5641 cttcgccggt cccttcgtcg gcctcggggg cctcggacgc gtcttcgcgc atcgggggag
     5701 attcggagta tgcgcgcgac cgttcgtccg aggcctgcga ggccgtgccc gaggcggcct
     5761 cgggcgcggc cggctgctcg ccgtcctgcg tcctgcgctc ctcctcgggc gtccgtgcct
     5821 cgtcggtcat cgtcccgcct tgatctcgat gcgtcgcggc ttgacggcct ccgccttggg
     5881 gacgtcgatg gtcagaagac cgttgtcgaa cgacgcttcg atcctgtccg cctgcacctg
     5941 cgtcggaagc gccaccgtgc gctggaacgc gccgaagcgg cgctccacgc ggtggaagct
     6001 ctcctccgcg acgtcggtgt agaacttgcg ctcacccttg accgtgagcg caccgtcggt
     6061 gacggtgacg tccacctgat cgggtccgaa gccgggcagc tcgatgacga cgcggtagcc
     6121 gtccggcgtc tcggcgatgt cgaccggcgg cgcccacatg cgcgctccgg ttccgttgtc
     6181 gccgtacgca cggtcgaaca agcgtgtcat ctcggtttgg ataccgagca gatcccggaa
     6241 gggatcccac ttgctcaggt tcgtcatggt atccacctcc tcggggtttc ccccactcgt
     6301 ccggcggccc tgcgccgccg gaatcctgcg aacgaacgcg atcgtcatgc gccctcaccc
     6361 tcgtccacga tctcggcgtc gacgacgtcg ccctgcggcg gctcgttcgc ggcctggcct
     6421 tgctccgccc cggccgcacc cgcggcctgc tgcgacgcgt acatcgcctg cccgatcttc
     6481 tgcgcggcgg tcgagagccg tccggccgcg tcgttgatcg cctgtgcgtc cgagcccttg
     6541 agcgcttcct tggtctcgtc gatcgcattc tggatctcgt ccttgtctgc ggccggcagc
     6601 ttgtcgccgt gctcaccgag catcttctcg gtctggtaga cgaggttgtc ggcctggttg
     6661 cgcgcctccg cttcggcgcg gcgtttcttc tcgtcgtcgg ccgacgcctc ggcttcgcgc
     6721 atcatccggt cgatctcgtc cttcggcagc gcggtgccgc cggtgatcgt catcgcctgc
     6781 tccttgccgg tcgcggtgtc cttcgccgag acctgcacga tgccattggc gtcgatgtcg
     6841 aaggcgacct cgatctgcgg catgccgcgc ggcgccggcg cgatgccgac gagctggaac
     6901 ttgccgagcg aacgcaccgc cggcgacgtg gccatctcgc cctcaccctg cagcacgtgg
     6961 atctcgacgg tcgtctgacc gtcctcggcc gtggtgaaga cgtcgctctt tcgcgtcggg
     7021 atcgtggtgt tgcgttcgat cagcttggtg aagatgccgc ccttggtctc gacgcccagc
     7081 gtcagcggcg tgacatcgag cagcagcaca tccttgacct cgcccttgag cacgccggcc
     7141 tggatcgcgg cgccgaccgc aacgacctcg tcggggttga cgcccttgtg cggatccttg
     7201 ccgccggtga gctccttgac caggttctgg atcatcggca tgcgcgagga accgccgacc
     7261 aggatgacgt ggtcgatgtc cttgacgtcc atgcccgcat ccttgatggc gaggttgaac
     7321 ggcacacggc agcgctcgac cagatcggcg gtcatgcgct cgaattcggt gcgcgtgagc
     7381 ttgagctcca tgtgcagcgg gccgtcggcg gtcgcggtga tgaacggcag gttgatcgtg
     7441 gcctcggtgg tctgcgacag ctcgatcttg gccttctcgg cggcttcctt cagccgctgc
     7501 agcgccatgc ggtcgttgcc caggtcgacg ccgtggttgt tcttgaactg cgcaaccatc
     7561 cagtcgatga cgcgctggtc ccagtcgtcg ccgccgagct gcgtgtcgcc gtgcgtcgcc
     7621 ttgacctcga acacgccgtc gccgatctcg agcagcgaca cgtcgaacgt gccgccaccg
     7681 aggtcgaaga ccagaacggt gtgatcggtc gtgtttttgt cgaggccgta ggcgagcgcg
     7741 gctgcggtgg gctcattgat gatgcgcagc acctcgaggc cggcgacctg cccggcttcc
     7801 ttggtggcct gccgctgcgc gtcgtcgaag taggcgggca cggtgatgac ggcctgcgtg
     7861 accttgtcgc cgaggtagga ctcggcgtcg cgcttgagct tcatcagcac ctgcgcactg
     7921 atctcctgcg gcgtccactt cttgccgtcg atgtccagcg tccagtcctt gcggcccatc
     7981 tcgcgcttta tggagcggac ggtgcggtcg gggttggtga tcgcctgacg cttggccacc
     8041 tcgccgacca ggatctcgcc ggtcttcgag aaggccacga ccgacggggt cgtgcgcgcg
     8101 ccctcggcgt tcgcgatgac ggtgggatcg ccaccttcga gcactgcgac gaccgagttg
     8161 gtcgtgccca ggtcgatgcc tacgactttg gccatgttca gatctccctc gtccactgcc
     8221 ggaccccggg cccacgcctc gggaagcttg acgaaggaga gtttaagaac ttgagcctgt
     8281 ctatgtcaag tttcatgctc gccacaggct aaagacggga aaacccctgg tggaatagca
     8341 gaaggccggg cagattgtcg ggattgtcgg ctaacgtggg gggccttcag aggcccgccg
     8401 ggccttgcgt gctgcctgct cctcggcgag ccgccgctct gcctcctcga acgtcggagc
     8461 cgctccgccg agatgggcgg gaagccacca ccggtcggca tcgccccgag gcttcgccgg
     8521 gtagatgcgc tgcgcgtgct ccagcagctc ggtgatccga gccatgaggc gcgtggtcgc
     8581 ggcctcggcg tcctcaccct cgatcatctc gagcggctcg cccaccacca ccacgatcgg
     8641 gatgttgcgc ttgaagttcc gcggccgcca cttcgtcagg atccgctgag tgccccagac
     8701 cgccaccggg atgatcggcg cgccggtctc gcgcgccgcg cgaaccgtgc ccgtctttcc
     8761 cttgcgagga acgaacgacg ggctgatcgt cgcctccggg aacatcccga tgacctcgcc
     8821 cgcctgcagc gccgtgacgg cggcccgcac gctcgcgtcg gcggcaccgt gccggtccac
     8881 cgggatgtgg cccatctgcc gcatgaacca gccgacgccg cccttgtcga agatctcctt
     8941 cttggccagg aagcgcacgc gccggccctg ctcgcgcgcc gcgtagccgc cgaacacgaa
     9001 atccagatac ccgatgtggt tcgacgccag gatcgccgga ccgctcgccg gcacgtgctc
     9061 gccgcccacc acggtcaccg gccacctcat cgacttgatc atgccgagcg caccggcgat
     9121 gaccacgctg tagacccgct ctcccatgct cgctcccgtt cggtgccgct ttgactgtcg
     9181 cgaggctacc gccgcgcgcg tccggtttca catgcggccc ggccgaacgg gtcacaccgg
     9241 ctgcgggacg cggccttcga cctcgacccg cgctcccggt ccgaagccgg ccagagcggt
     9301 cagcggcacc tcgagcgccc gatcgtaggg ctcgtcggga atggtgagcg ggcagggatc
     9361 ggccgtgcag ggctgcatct cgcggacatc caccacggtg ccggcacgaa tgaagaagat
     9421 tcgaagcggc acgagcgtgt tcttcatcca gaagccgccg cgcgtgggag acggaaagac
     9481 gaagagcatg ccgcgtgcct ccgggatctc gcggcgtccc atcagcccgg tcgcccgctg
     9541 atcgggcgtg gacgcgacct cggcggtgac caccgcgcgc cgcgcgcctt cgatgatcgc
     9601 gacgcgaacc tcgccgtcgc cgttcgagcc gccgcacgcg gcgagcagca ggatcagccc
     9661 aacgaacagg gctcgggcgg atcggcgcat cgccgcatgg tatgcccgcc gcgcgccggc
     9721 cggtatgctc ggcctcatgc acgtggccgt caccgccgtg ggccgggatc gtcccggcat
     9781 cgccgccgcc gtcagcaagg tcctgttcga gcacggcgcc aacatcgagg actcgcgcat
     9841 ggcgatcctg ggcgggcatt tttcgatgat gctgatcgtg gcgaccgacg ccgacgcgca
     9901 gacgctcgag cgagacctcg cggccgcgac cgcgagcctg gacctgaccg ttgcggtgcg
     9961 cgcggtcgag gacgcggcgt ccgcggcgtc cgaaggcgcg gcgttcacgc tgtcggtcta
    10021 cggcgccgac catcctggga tcgtctaccg ggtgaccgag gcgctcgcgg aacgctcgat
    10081 caacgtcacc gatctcgcca cccgcgtgat cggagacgac ccgcccgtct acgtgatgat
    10141 catggaggtc accgtgcccg ccgggctgaa cgccgcagcc ctcgacgacg gcctcaagca
    10201 tctgggagcc gacctcggcg tggacgtgtc gctgcggccg gccgaggccg acacgctgta
    10261 gcggtggccg ttcgcgacgt cgttcgctac ccggccgcgg tgctcaagag cccggccgcg
    10321 accctcgatc cgggacccga ggccgaagcc atcgccgcgg atctgatcga gacgatgcgt
    10381 gcctctccgg cttgcgtcgg gcttgcggct ccgcagatcg ggctcggcgt gagcgcgttc
    10441 gcactcgacg tgaccggcca caagaaagcc cgatcgtgcg ccggccttgt cgtgatgatc
    10501 aaccccgaga tcgtcgcgca cgacggcagc gagctggcac gcgaagggtg catgagcttg
    10561 cccgacttca ccggcaacgt gcgccgagcg caatgggtcc gcgtgcgcgg cctgtcgccg
    10621 gcgggcgaga cggtcgagat cgaggcggac gcgttcgagg cccgcgcgtt ccagcacgag
    10681 atcgaccacg tcaacggcct gctgttcctc gaccgggtcg tgtcgatgac ctcggacgtg
    10741 ttcgagcgca agcgctaccg ctagcgagcc ctgatcagcc gccggcgccg agcttcgcca
    10801 gctcggagta gtccgaagtc tggtcggccg gaggcgccga gacctcgacc ttggttccga
    10861 agtcgaacag gtccatcccg atcgtcatct cgccgcccgc cgccgagctc tcgccctcgc
    10921 cctcatcggt g
//
LOCUS       DATLFO010000127         5660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_103884, whole genome shotgun sequence.
ACCESSION   DATLFO010000127 DATLFO010000000
VERSION     DATLFO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103884"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VM841_04690"
     CDS             <1..517
                     /locus_tag="VM841_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89516.1"
                     /translation="LYTTGVLWQPGLEWYRITTLAYREDFPTATLTRVARWVQEGKHA
                     AASRAMEYFSIGDFQRWAQAQDPSSLGATRAQWEGASDSGRAYGRAWIDTSSDPPVGE
                     ENFVGAITSLQRDVAAGNYALVRDYTGVRISLFDGFIRAQYYISHDRLIPQGGYVFVG
                     EAGIGIPPPPV"
     gene            complement(573..646)
                     /locus_tag="VM841_04695"
     tRNA            complement(573..646)
                     /locus_tag="VM841_04695"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(612..614),aa:Cys,seq:gca)
     gene            complement(691..765)
                     /locus_tag="VM841_04700"
     tRNA            complement(691..765)
                     /locus_tag="VM841_04700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Gly,seq:gcc)
     gene            complement(840..1271)
                     /gene="dut"
                     /locus_tag="VM841_04705"
     CDS             complement(840..1271)
                     /gene="dut"
                     /locus_tag="VM841_04705"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVL89517.1"
                     /translation="MKLSIKRLDPGIDLPVQANRGDAGLDLRSTLDLTIKPGERAVVP
                     AGIAVAIPEGYAGFVQPRSGLAARHGLGIVNSPGLIDAGYRGEIKVVLVNLDPSEAVE
                     ISRGDRIAQLVVLPVPQVQVIEVDELPGSERGESGFGSSGR"
     gene            complement(1349..4078)
                     /locus_tag="VM841_04710"
     CDS             complement(1349..4078)
                     /locus_tag="VM841_04710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HVL89518.1"
                     /translation="MAKTERAMEKGLKVKRYFTTQGKHPYDEIEWDVRDAIIPNYKDG
                     GNAFEQRAVEFPSLWSMNATNIVAQKYFRGPLGSPQRESSVRQLIDRVADTITAWGWK
                     DGYFATEEDRQTFNAELKHILVTQKAAFNSPVWFNVGSEDHPQCSACFILAVEDKMSS
                     ILNWYVEEGTIFKGGSGSGINLSTIRSSKERLSSGGEASGPVSFMRGADASAGTIKSG
                     GKTRRAAKMVILNVDHPDVEHFVWCKAVEERKARVLRDNGFDMDLDGSDSHSIQYQNA
                     NNSVRVTDDFMQAYLNDADWDLRAVKTGEPVEQIRARHLMRQIATAAWECADPGMQYD
                     TTINDWHTCPASGRINASNPCSEYMHLDNSACNLASLNLMKFLDADLNFDIQAFRHAV
                     DIIFLSQEILVSNSSYPTEAIGKNAEDFRELGIGYANLGALLMSRGLPYDSEGGRAWA
                     GAITALMTGWAYRVSAKVAEKKGPYNGYPPNREAHLRVMRKHRAAVDDIDPELVPEGL
                     LQAAKGSWDDAITMGEEHGYRNSQASVLAPTGTIGLMMDCDTTGIEPDLALVKTKKLV
                     GGGTMKIVNQTVPRALTRLGYNQQQVEEITAYIHEHNNVEGAPHLRPEHYPVFDCAMG
                     ERTIHYMGHVKMMAAVQPFISGAISKTINMPESATVEDVEDLLVEGWRLGLKAVAIYR
                     DNCKVAQPLSADKKKTAAAPAASAEPVDAPKTLADRKRLPKSRPSRTISFRVGDQEGY
                     MTAGMYPDDGVGEVFLKVSKQGSTVSGVMDALAIAVSIGLQYGVPLEAYVSKFINMRF
                     EPLGMTDDPDIRMAQSLVDYIFRRLAIEFLPEEVRADLGIRTTEERKDEVAGKAQPQA
                     ATVEITPAPAGSGPAAPSVKAADAPYCYNCGNQMRPAGSCFVCESCGSTSGCS"
     gene            complement(4158..4715)
                     /gene="nrdR"
                     /locus_tag="VM841_04715"
     CDS             complement(4158..4715)
                     /gene="nrdR"
                     /locus_tag="VM841_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HVL89519.1"
                     /translation="MDNGDAPADGIAIERVRVRCPYCRSVEDKVVDSRMAEEGAAIRR
                     RRECLACGKRFTTFERAEGVTLWVVKRSGERVAYDRSKVVEGLRRACVNRPVASEEIQ
                     RVADEIEEAMRAPGVNEVRSQDVGIAVLDRLRELDEVAYIRFASVYKDFQELTDFERE
                     VGLLLQKKTPPKSSGPGSQTNGPDG"
     gene            complement(4848..5120)
                     /locus_tag="VM841_04720"
     CDS             complement(4848..5120)
                     /locus_tag="VM841_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVL89520.1"
                     /translation="MKGLKRFWIAIPLAVGILIGGAANVTARQDHPAPFRGTVHQVRL
                     GETLWEIAKDAHPGSDPRRMVSRIKSENGLRSSVVRPGQKLRIPAP"
     gene            5304..>5660
                     /gene="lexA"
                     /locus_tag="VM841_04725"
     CDS             5304..>5660
                     /gene="lexA"
                     /locus_tag="VM841_04725"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVL89521.1"
                     /translation="MSDLTKRQQQILEFIRTVTEDRGYPPSVREIGEAVGLNSPSSVH
                     AQLATLARAGYLSKDPSKPRAIVVRMDSEGMPVEAGNVEHVPLIGRIAAGGPILATEN
                     LEDTLPLPRDLLGPGTL"
BASE COUNT          910 a         1937 c         1783 g         1030 t
ORIGIN      
        1 gctctacacg accggtgtcc tgtggcagcc gggcctggag tggtaccgca tcaccacgct
       61 cgcctaccgc gaggacttcc ccaccgcaac gctgacccgc gtcgcgcgct gggtccagga
      121 aggcaagcat gccgccgcgt cccgagccat ggagtacttc tcgatcggcg acttccagcg
      181 ctgggcgcag gcgcaggacc cgtcgtcgct cggtgcgact cgcgcgcaat gggagggcgc
      241 gtcggattcg ggccgcgcgt acgggcgcgc atggatcgac acgtcgtcgg atccgcccgt
      301 gggcgaggag aacttcgtcg gggctatcac ctcgctgcag cgagacgtcg cagccggaaa
      361 ctacgcactg gtccgcgact acaccggcgt ccgaatctcg ctcttcgacg ggttcattcg
      421 cgctcagtac tacatctcgc acgaccggct gatcccgcaa ggcggctacg tgttcgtcgg
      481 cgaggccggg atcggcatcc ccccaccgcc ggtctgattc cgttgctgaa tccggaaatg
      541 acgaaacccc tgctcacgcg ggggtttctg tttggaggcg gcgtccggaa ttgaaccgga
      601 gtgcagggat ttgcagtccc ttgcctaacc actcggccac gccgccagac ttcgggccgg
      661 cacaagtgcc ggcccgaagt tgtttcgata tggagcgggc gaccagattc gaactggcga
      721 ccctcacctt ggcaaggtga tgctctacca ctgagccacg cccgcgtgga acggaaccgc
      781 gattgtacca accttgcggc ggcctgcacc acatgcaccg cagaacggga aaatccctgc
      841 tagcggccgc tcgatccgaa cccgctttca ccgcgctcgc tcccgggcag ctcgtcgacc
      901 tcgatgacct ggacctgcgg aacgggcagg acgaccagct gcgcgatacg gtccccccgg
      961 gatatctcga cagcctcact cgggtccagg ttgacgagca cgaccttgat ctcgccccga
     1021 taacccgcgt cgatcaggcc gggcgagttc acgatgccga gcccgtgccg tgcggccagc
     1081 cccgatcgtg gctgcacgaa ccctgcgtac ccctctggga tcgccaccgc gatccccgcc
     1141 ggcacgacgg cccgctcccc cggcttgatc gtcagatcca gcgtggagcg aagatccagc
     1201 ccggcatccc cccggtttgc ctgaacgggc agatcgattc ccggatccag cctcttgatc
     1261 gaaagcttca tggctcccct tgaaatatgt cgcgcccccg actcgtgggc cgcacgaatg
     1321 cggagccggg ggcgcgacga ctatcggatc agctgcagcc ggacgtcgat ccgcaggatt
     1381 cgcacacgaa gcacgagccg gcgggccgca tctggttgcc gcagttgtag caatagggcg
     1441 catcggcggc cttgaccgac ggcgcggccg gacccgatcc ggccggagcc ggggtgatct
     1501 cgacggttgc cgcctgcggc tgcgccttgc ccgcgacctc gtccttgcgc tcttccgtgg
     1561 tccggatgcc gagatccgcg cgcacctctt cgggcagaaa ctcgatcgcc agacggcgga
     1621 agatgtagtc caccagcgac tgcgccatcc ggatgtcggg atcgtcagtc atgccgagcg
     1681 gctcgaagcg catgttgatg aacttcgaca cgtaggcctc gagcggcacg ccgtactgca
     1741 gcccaatcga cacggcgatc gccagcgcgt ccatgacacc tgagacggtc gagccctgct
     1801 tggatacctt caggaagacc tcgccgaccc cgtcgtcggg gtacatgccg gcggtcatgt
     1861 agccctcttg gtcgccgacg cggaacgaga tcgtgcgcga cggacgcgac ttcggcagac
     1921 gcttgcggtc cgcgagcgtc ttgggcgcgt caaccggttc ggcgctcgcg gccggcgcgg
     1981 ccgcggtctt cttcttgtcg gccgacaggg gctgggcgac cttgcagttg tcgcggtaaa
     2041 tcgcgacggc cttcaagccc agacgccatc cttcgaccag caggtcctcg acgtcctcga
     2101 ccgtcgcgga ctcgggcatg ttaatggtct tggagatcgc gccggagatg aacggctgca
     2161 ccgccgccat catcttgacg tggcccatgt agtgaatggt gcgctcaccc atcgcgcaat
     2221 cgaacacggg atagtgctcg ggccgcaggt gcggcgcgcc ttcgacgttg ttgtgctcgt
     2281 ggatgtaggc ggtgatctcc tcgacctgct gctggttgta gccgaggcgc gtcagcgcgc
     2341 gcggcacggt ctggttgaca atcttcatcg tgccgccgcc gaccagcttc ttggtcttga
     2401 ccagcgcgag gtccggctcg atgccggtgg tgtcgcagtc catcatcaga ccgatcgtgc
     2461 ccgtcggggc gagcaccgag gcctgcgagt tccgatagcc gtgctcctcg cccatggtga
     2521 tcgcgtcgtc ccacgagccc ttggcggctt gcagcagccc ttcggggacc agctcggggt
     2581 cgatgtcgtc caccgcggcg cggtgcttac gcatcacgcg caggtgcgcc tcgcggttcg
     2641 gcgggtagcc gttgtacggg cccttctttt cggcgacctt cgcgctgacg cgataggccc
     2701 agccggtcat cagcgccgtg atcgcgccgg cccacgcgcg gcccccctcg ctgtcgtagg
     2761 gcaagccacg gctcatcagc agcgcgccca ggttggcgta gccgatgccg agctcgcgga
     2821 agtcctcggc gttcttgccg atcgcctcgg tcgggtacga cgagttcgac accaggatct
     2881 cctgcgacaa gaagatgatg tccaccgcgt ggcggaacgc ctggatgtcg aagttcaggt
     2941 cggcgtcgag gaacttcatg aggttcagcg acgccaggtt gcacgccgag ttgtccaagt
     3001 gcatgtactc gctgcacggg ttcgacgcgt tgatgcgccc ggacgccggg cacgtgtgcc
     3061 agtcgttgat ggtggtgtcg tactgcatgc ccgggtcggc gcactcccac gctgccgttg
     3121 cgatctggcg catcagatga cgggcgcgga tctgttcgac cggctcgccg gtcttgaccg
     3181 cgcgcagatc ccaatcggcg tcattgaggt acgcctgcat gaaatcgtcg gtgacgcgga
     3241 ccgagttgtt ggcgttctgg tactggatcg aatgcgagtc gctgccgtcc agatccatgt
     3301 cgaagccgtt gtcgcgcaac acacgcgcct tgcgttcctc gacggccttg caccagacga
     3361 agtgctcgac gtcgggatga tccacgttca agatcaccat cttggccgcg cggcgggtct
     3421 tgccacccga cttgatcgtg cccgcactgg cgtccgcgcc gcgcatgaac gacaccggcc
     3481 cgctcgcctc gccgcccgag gagagccgct ccttcgacga ccggatcgtg gacaggttga
     3541 tgcccgagcc cgatccaccc ttgaagatcg ttccctcttc gacgtaccag ttcaggatcg
     3601 agctcatctt gtcctcgacg gccaggatga agcacgcgct gcactgggga tgatcttcgc
     3661 tgccgacgtt gaaccagacg ggagagttga atgcggcctt ctgcgtgacc aggatgtgct
     3721 tgagctcggc gttgaaggtc tgccggtcct cctcggtggc gaagtagccg tccttccaac
     3781 cccaagcggt gatggtgtcg gcgacccggt cgatcagctg gcgaacggag ctttcgcgct
     3841 ggggagagcc gagcggcccg cggaagtact tctgcgccac gatgttggtg gcgttcatcg
     3901 accacagcga ggggaactcc accgcgcgtt gctcgaaggc gtttccgccg tccttgtagt
     3961 tcgggatgat tgcgtcccgg acgtcccact cgatctcgtc gtagggatgc ttcccctgcg
     4021 tggtgaagta ccgcttgacc ttgaggccct tctccatcgc tcgttccgtc ttggccactg
     4081 tgcccctccc ctgaaccgtt ctctcgccgt caaccaggtc ctgtcgcgtt ccggcccgcc
     4141 ggtatccgtc ctcgccgcta tccgtcgggg ccgttcgtct gggatccggg gcccgaggac
     4201 ttcggaggcg tcttcttctg cagcagcagg cccacctcgc gctcgaagtc ggtcagttcc
     4261 tggaagtcct tgtagacgct ggcgaaccgg atgtaggcga cctcgtccag ctcgcggagg
     4321 cgatccagga cggcgatacc gacgtcttgg ctgcggacct cgttcacacc cggggccctc
     4381 atcgcttcct cgatctcgtc cgccacgcgc tggatctcct cgctcgccac cggccggttc
     4441 acacacgccc tccgaaggcc ttcgacgacc ttgctgcggt cgtaggcaac ccgctcaccg
     4501 gagcgcttca ccacccagag cgtgacccct tcggcccgct cgaaggtcgt gaaccgcttt
     4561 ccgcatgcca ggcattcgcg tcgcctccgg atcgcggcgc cttcctcggc catccgcgaa
     4621 tccacgacct tgtcctcgac cgacctgcag taagggcacc gcacccgaac cctttcgatc
     4681 gcgatcccat ccgccggcgc atccccgttg tccaccgctg tggacaacat cagggactac
     4741 atgttgggga taccagccgg aaaccacaat atcttgtggt tggagagaat ctatcacaca
     4801 agatacgcgc gttcaaggat catggaagag gcggagaaaa ctgctggtca gggcgccgga
     4861 attcgcaact tctgccccgg gcgcaccacc gacgaccgca acccgttctc ggacttgatc
     4921 ctcgagacca tgcgcctcgg gtccgagccg gggtgggcgt ccttggcgat ttcccacagg
     4981 gtttccccaa gccggacctg gtggacggtg ccccggaacg gcgccgggtg gtcttggcgc
     5041 gcggtcacgt tggccgcccc gccgatcagg atccccacgg caagcgggat cgcgatccag
     5101 aagcgtttca ggcccttcat gctgctcagc tccttcgatc gatcgagact cttccggtcg
     5161 aacccaggct agacccgggc accgacacta tcgaacaggc gttcggtccg gtcaaggccg
     5221 aaatcgaaca gatgtttgat ccgaacgcac gttcgtggta gcgtttgctc gctttccgcc
     5281 gacacggccg accgggagga ccgatgagcg acctgaccaa gcgccagcaa cagatcttgg
     5341 agttcatccg gaccgtcacc gaagaccgcg gctatccgcc gtccgtccgg gagatcggtg
     5401 aggccgtggg cctgaactcc ccttccagcg tgcacgcgca gctcgccacg ctggcccgag
     5461 cgggctacct atcgaaggat ccgtccaagc cccgggcgat cgtggtgcgc atggactccg
     5521 aaggcatgcc cgtcgaggcc ggcaacgtcg agcacgtccc actgatcggc cggatcgcgg
     5581 caggcgggcc gatccttgcg accgagaacc tggaggacac gctgccgctc ccccgggacc
     5641 tgctcgggcc gggcacgctg
//
LOCUS       DATLFO010000128         7789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_83664, whole genome shotgun sequence.
ACCESSION   DATLFO010000128 DATLFO010000000
VERSION     DATLFO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83664"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VM841_04730"
     CDS             <1..1063
                     /locus_tag="VM841_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL89522.1"
                     /translation="AAARAGSPEHRIGDSPRGRRLFVAAPIPSMNGVLWVSLSLDPVE
                     RLNRETWIVSATVGALMLVVAAIVGSTLASRITRRLEQVADGAARFGEGHWDERIPVE
                     GPDEIAALAARLNEMGSEIGRLVRREKDFVAAASHQLRTPLAAIKIRIDEIRALGAIT
                     DAQALEDLDEMSEEVDRLTALTRRLLDLSAAEAALEARPVPVGPAVREVVGRVGPLAE
                     LRRIAVLVGDDPDDAVIAAPTGAFEEVLFNVLDNAVKYSAPGAHVDVRVSGGDDRVVV
                     SVADRGPGVPDGERDRVFAAFYRGSRKEPGHGLGLAIGARLCARSSASLALEPRPGGG
                     TVARIEWPAWTPAPPPEAG"
     gene            1122..1964
                     /locus_tag="VM841_04735"
     CDS             1122..1964
                     /locus_tag="VM841_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89523.1"
                     /translation="MTGPSTQTDARRALLGALFDDAGLFPPASLAMPDAVAGHERART
                     GPHAWMLGRFVCPAVKLASLIEHAAGSPVRVSVILDTPDAAMSAGDAADRSGGRIVIE
                     QSEAKLPAVPPEDAVAGLLARAGEWPLYVEIPAAALAVIEIVNGQPGIGVKIRCGGDA
                     MPSPESIAATMVACRDARVPMKATAGLHHPYRTESQHGFLNICAAAVAAWTGADQVEV
                     ASIVDERDTSAITLTAASLTVRGRDFDAAACVGAREHLFASIGSCSFDEPVEDLIALG
                     VLPA"
     gene            1961..3208
                     /gene="fahA"
                     /locus_tag="VM841_04740"
     CDS             1961..3208
                     /gene="fahA"
                     /locus_tag="VM841_04740"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HVL89524.1"
                     /translation="MSDRIRTWVPNAEDSGYPIESLPLGIFRAGGAPRAGAAIGDSIL
                     DLTACDEDGLLPVPRGTFAGDTLNGLLSLGAGAWRALRARLAALLAEGNDEIRLARPG
                     ALVSMREATMQMPVAVGDYVDFYSSIQHATNLGRIFRPDAEPLLANWRHLPVGYHGRS
                     GTIVVSGTPVRRPAGQRKPPDADAPSFGPSVRLDFELELGFVTGDANMLGRAVPVAAA
                     EEHIFGVVLVNDWSARDIQAWEYQPLGPFLGKSFATSISSWVVPLDALAPYRVPAPPQ
                     EPPPLPYLRCDGDRVLDADLSVELTAGGGAPTVIARTNARNLYWTMAQQLAHATVNGA
                     TARAGDLFASGTISGSEAGTFGSMIELSWNGAAPVPLQGGEERAWLEDGDTVVMRGVC
                     GGYDGLPRIALGEVSGTVLPAES"
     gene            3266..4699
                     /locus_tag="VM841_04745"
     CDS             3266..4699
                     /locus_tag="VM841_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HVL89525.1"
                     /translation="MRFDGYDLGGFYDEMFQAPGVPREAYRALIDRIEGLSEGELLRR
                     QRAAEAALMDLGVTFNAGTDGGTEKIFPFDLVPRIIPAEDWEPIERGLAQRIMALNLF
                     LDDVYHDQRIVKDGIVPAHLVEGAKTYLPACEGLNPPGGVWCHVTGTDLIRDRDGRWF
                     VLEDNLRSPSGVSYVLENRQLLKRTFPAVFAAAGVRAVEDYPSRLLETLQRLAPAAVS
                     NPVVVVLTPGLYNSAYFEHAFLARQMGVELVEGRDLVVEGGFVTMRTTKGSRRVDVIY
                     RRIDDDFLDPLTFRPESALGVPGLMDVYRAGRVALANAPGTGIADDKVIYAYVPQMIR
                     YYLEQDPILPNVPTYVCGEDEQRRYVIAHLDELVVKTANDSGGYGMLIGPHATAEERD
                     EFARRIEANPRNYIAQPTIALSRVPVITEDGSFAGRHVDLRPFVLHGETPWVMPGGLT
                     RVALTPGSLVVNSSQGGGSKDTWVLSR"
     gene            4702..5673
                     /locus_tag="VM841_04750"
     CDS             4702..5673
                     /locus_tag="VM841_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HVL89526.1"
                     /translation="MLSRVADAIYWMNRYLERADNIARFIDVNYHLTLDVRTGDEQWL
                     PLIATTAALPQFHARYDKPTRANVIKFMAFDRENPNSILSCVERARENARSVRDQITI
                     ETWEEINGFYRMVRHEASLGSIGSDPYRFLASVKRTGSLIQGVVDATTSRSEGWHWGR
                     IARMLERADKTSRILDVKYFILLPNVTDVGTPLDDLQWTALLKSASALEMYRKRYGRI
                     APERVAGFLLLDREFPRSVQFCVHLADLSLRRITGTQSETFSNKAEQMLGRLGSMLAY
                     SGIGEIVAAGLHEFLDQLQRDLNTVDDAIYEGFFALRPAGVARGGNQ"
     gene            5670..6440
                     /locus_tag="VM841_04755"
     CDS             5670..6440
                     /locus_tag="VM841_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HVL89527.1"
                     /translation="MTFCLGMKVRDGIVGIADTRITSGAEAITARKISIREHDRHSLF
                     MMTSGLRSVRDKALTYFDEIVARDEPAFTRLYQVANAFAEQVRRVAKEDGEALAEAGL
                     AFNLHTLVGGQLDEDAEPMLYMLYPQGNWVEVTRGTPYFIIGETGYGKPLLDRALGYD
                     APLEQALKIGYLAFDATRTSATDVDFPIDIVVYRNDSFSMREHRYEFEELSEVSQMWN
                     RNLRRLIDEVPSRWLEAVFPSVTKPDNVRPMPGASRES"
     gene            6453..7286
                     /locus_tag="VM841_04760"
     CDS             6453..7286
                     /locus_tag="VM841_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HVL89528.1"
                     /translation="MRFRVRHLTRYGYDRAVPLGALTVRLRPRDDGTQRLASFSLACD
                     PAPSGRSEMLDAEGISVTRLWFEGETSSLTVETSFEAETLRANPYDWVIDPGICVAPM
                     RYGPREAAALTRELRFGGDPAAADLSDEILKESAGSASAFLSLLCSRIAATVVHERRP
                     EGPPRLPSETLSAGHGACRDVAVTYVESARAAGFAARFVSGYTTPEDGFPGELHAWAE
                     VYLPDGGWRGYDATGGIAVADTHVAVAAAADPAATAPVEGTYGAGAASALKTVVDIET
                     S"
     gene            complement(7339..>7789)
                     /locus_tag="VM841_04765"
     CDS             complement(7339..>7789)
                     /locus_tag="VM841_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89529.1"
                     /translation="FADGVSNTDQSLIAVGNSLIAENNYGYSGPLATQSGGVTEPGLA
                     RVDVDPSAGTCTVAWTSNERAPSVVPKVSLANGLVYTYTKDPQPDGSDAWYFTALDFR
                     TGATVYKVRTGLGLGFNNNYAPVTLGPDGTAYVGVLGGLIAITDSSS"
BASE COUNT         1139 a         2697 c         2744 g         1209 t
ORIGIN      
        1 cgcagccgca cgcgccggct cgcccgagca tcgcatcggc gactcgccac gcggacggcg
       61 gctcttcgtg gccgctccca tccccagcat gaacggcgtg ctgtgggtgt cgctgtcgct
      121 cgatccggtg gagcggctca accgcgagac ctggatcgtg tcggcgaccg tcggcgcgct
      181 catgctcgtg gtcgcggcca tcgtgggctc gacgctcgcg tcgcggatca cccggcggct
      241 cgagcaggtc gccgacggcg ccgcccgctt cggcgaagga cactgggacg agcgcatccc
      301 cgtcgaagga cccgacgaga tcgcggcgct cgccgcgcgc ctcaacgaga tgggctccga
      361 gatcggccgt ctggtgcggc gcgagaagga cttcgtggcc gcggcctcgc atcagctgcg
      421 cacgccgctt gctgcgatca agatccggat cgacgagatc cgggcgctgg gcgcgatcac
      481 cgacgcgcag gcgctggagg acctcgacga gatgagcgaa gaggtggacc ggctcaccgc
      541 gctgacgcga cgcttgctgg atctgtccgc cgccgaggcc gcgctcgaag cgcggccggt
      601 gccggtcggc ccggcggtgc gcgaggtcgt agggcgcgtc ggcccgctcg ccgagctgcg
      661 gcgcatcgcg gtgctcgtcg gcgacgatcc cgacgacgcc gtgatcgccg cgcccaccgg
      721 cgcgttcgag gaggtcctgt tcaacgtgct cgacaacgcg gtcaagtatt cggcgcccgg
      781 cgcgcacgtg gacgtgcggg tatccggcgg ggacgatcgg gtcgtggtct cggtcgccga
      841 ccgcggtccc ggcgtgcccg acggcgagcg cgaccgggtc ttcgccgcgt tctaccgcgg
      901 ttcgcgcaag gagccgggtc atggtctcgg tctggccatc ggcgcacggc tgtgcgcccg
      961 gtcgtccgcc tcgctcgcgc tcgagccgcg tcccggcggc ggaacggtcg ctcgtatcga
     1021 atggcccgca tggacgcctg cgccgccgcc cgaggccgga tagcaccggt ccagcggggc
     1081 ttcccgcgcg atcgtgggcg gcgccggtac gcttggacgc cgtgaccggc ccgtcaacgc
     1141 agaccgacgc gcggcgcgcg ctcttgggcg cgctcttcga cgacgccggc ttgtttccac
     1201 ccgcgtcgct ggcgatgccc gatgccgtgg cgggacacga gcgcgcacgc accggcccgc
     1261 acgcctggat gctcggccgc ttcgtgtgcc cggccgtcaa gctcgcctcg ctgatcgagc
     1321 acgccgccgg gagcccggtt cgtgtcagcg tgatcctgga cacgcccgac gcggccatga
     1381 gcgccgggga cgcggccgac cggtccggcg gccggatcgt catcgaacag tccgaggcga
     1441 agctgcccgc ggtaccgccc gaggatgcgg tcgcgggcct gctcgcgcgc gccggcgaat
     1501 ggccgcttta cgtcgagatc cccgccgctg cgctcgcggt catcgagatc gtgaacggcc
     1561 agccgggcat cggggtgaag atccgctgcg gcggggacgc gatgccgtcg ccggagtcga
     1621 tcgccgcgac gatggtcgcc tgccgcgacg cacgcgtgcc gatgaaggcg accgccgggc
     1681 tgcatcaccc gtatcgcacg gagtcgcagc acgggttcct gaacatctgc gccgccgcgg
     1741 tcgccgcctg gacgggcgcg gaccaggtcg aggtcgcatc gatcgtggac gagcgcgaca
     1801 cgagcgcgat cacgctgacg gcggcctctc tgaccgtgcg cggccgcgat ttcgacgcgg
     1861 cggcgtgcgt gggtgcgcgc gagcatctgt tcgcgtcgat cggatcgtgc tcgttcgacg
     1921 agcccgtcga ggatctcatc gctctgggag tgctgccggc gtgagcgaca ggattcgcac
     1981 ctgggttccg aatgccgagg actcgggcta tccgatcgag agcctgccgc tgggcatctt
     2041 ccgcgccgga ggcgcgccgc gtgccggtgc cgcgatcggc gattcgatcc tggatctgac
     2101 ggcctgcgac gaggatgggc tgctgccggt tccgcgcggc acgttcgcgg gcgacacgct
     2161 caacgggctg ctctcgctcg gcgccggcgc gtggcgcgcg ctgcgcgcgc gcctcgccgc
     2221 gctgctcgcc gagggcaacg acgagatccg gctggcccgg cccggagcgc tggtgtcgat
     2281 gcgcgaggcg acgatgcaga tgccggtcgc ggtcggcgac tacgtcgact tctactcctc
     2341 gatccagcac gcgacgaacc tcggacgcat cttccggccg gacgccgaac cgctgcttgc
     2401 gaactggcgg catctgccgg tcggctatca cggacgctcc ggcacgatcg tggtgtcggg
     2461 cacgccggtc cgcaggcccg ccgggcaacg caagccaccg gacgcggacg cgccgtcctt
     2521 cgggccgtcg gtgcggctcg acttcgagct cgagctcgga ttcgtgaccg gcgacgcgaa
     2581 catgctcggc cgggcagtgc cggtcgcggc ggccgaggag cacattttcg gcgtggtgct
     2641 ggtcaacgac tggagcgcgc gcgacatcca ggcctgggag tatcagccgc tcggcccgtt
     2701 cctcggcaag tccttcgcga cgtcgatctc gtcgtgggtc gtgccgctcg acgcgctcgc
     2761 gccctaccgc gtgccggcgc cgccgcagga gccgccgccg ctgccgtacc tgcgctgcga
     2821 cggcgaccgg gtcctggacg ccgacctgag cgtcgagctg acggccggcg gcggtgcgcc
     2881 gacggtgatc gcgcggacga acgcgcgcaa cctgtattgg acgatggcgc agcagctcgc
     2941 gcatgcgacc gtcaacgggg cgaccgcgag agccggcgac ctgttcgcgt ccgggacgat
     3001 ctcgggctcg gaggcgggca cgttcggctc gatgatcgag ctgtcctgga acggcgcggc
     3061 gccggttccg ctgcagggcg gcgaagagcg cgcctggctc gaggacggcg acacggtcgt
     3121 gatgcgcggc gtgtgcgggg gctacgacgg gctgccgcgg atcgcgctcg gcgaggtctc
     3181 gggcaccgtg ttgcccgccg aatcgtagta cggcgggttt gccggcttgt tgtccccggg
     3241 ggcgcggctc cctgatgatg gcggtatgcg cttcgacggc tacgaccttg ggggcttcta
     3301 cgacgagatg ttccaggccc ccggcgtgcc tcgcgaggcc taccgcgcgc tgatcgaccg
     3361 gatcgaaggc ctatccgaag gcgagctgct gcggcgacag cgcgccgccg aggccgcgct
     3421 gatggatttg ggcgtaacct tcaacgccgg caccgacggc ggcaccgaga agatctttcc
     3481 cttcgacctg gtgccgcgca tcatcccggc cgaggactgg gagccgatcg agcgcggcct
     3541 cgcgcagcgg atcatggctc tgaacttgtt cctcgacgac gtctaccacg atcagcggat
     3601 cgtcaaggac ggcatcgttc ccgcgcatct ggtggaaggc gccaagacct acctccccgc
     3661 gtgcgagggc ctcaacccgc cgggcggtgt ctggtgccac gtgaccggga cggatctgat
     3721 ccgcgaccgc gacggacgct ggttcgtgct cgaggacaac cttcgcagcc cgtcgggcgt
     3781 gtcctacgtg ctcgagaacc ggcagctttt gaagcgcacg ttcccggccg tgttcgcggc
     3841 ggccggcgtg cgcgccgtcg aggattaccc gagccggctg ctcgagacgc ttcagcgcct
     3901 cgcgcccgcg gccgtgtcga acccggtcgt ggtggtgctc accccgggcc tctacaactc
     3961 ggcctacttc gagcacgcgt tccttgcgcg ccagatgggg gtggagctgg tcgaaggacg
     4021 cgacctggtc gtcgagggcg gcttcgtcac gatgcgaacg acgaaagggt cccgccgcgt
     4081 ggacgtgatc taccggcgca tcgacgacga cttcctcgat ccactgacgt tccggcccga
     4141 gtcggcgctg ggcgtgcccg gcctgatgga cgtctaccgc gccggccggg tcgcgctcgc
     4201 gaacgcgccc ggcaccggga tcgccgacga caaggtgatc tacgcctacg tgccgcagat
     4261 gatccggtac tacctggaac aggatccgat ccttccgaac gtgccgacct acgtgtgcgg
     4321 cgaggacgag cagcgccgct acgtgatcgc ccacctcgac gagctcgtcg tcaagaccgc
     4381 caacgactcg ggcgggtacg gcatgctgat cggcccgcac gccaccgccg aggaacgcga
     4441 cgagttcgcg cggcggatcg aggcgaaccc gcgcaactac atcgcccagc ccacgatcgc
     4501 gctgtcgcgg gtgcccgtga tcacggagga cggctcgttc gccggccggc acgtcgatct
     4561 tcggccgttc gtcctgcacg gcgagacgcc ctgggtcatg ccgggcgggc tcacccgcgt
     4621 ggcgctcacg ccgggctcgc tggtggtcaa ctcctcgcaa ggcggcggga gcaaagacac
     4681 ctgggtgctg tcgaggtagg catgctgagc cgcgtcgccg atgccatcta ctggatgaac
     4741 cgctatctcg agcgggcgga taacatcgca cgcttcatcg acgtcaacta tcacctgacg
     4801 ctggacgtgc gcaccggcga cgagcaatgg ctgccgctga tcgcgaccac cgccgcgctg
     4861 ccgcagttcc acgcccgcta cgacaagccc acgcgtgcga acgtgatcaa gttcatggcc
     4921 ttcgatcgcg agaacccgaa ctcgattctg agctgcgtcg agcgcgcccg cgagaacgcg
     4981 cgctcggtcc gcgaccagat cacgatcgag acgtgggagg agatcaacgg cttctaccga
     5041 atggtgcgtc acgaagcctc gctgggcagc atcggctcgg atccgtaccg cttcctggcc
     5101 tcggtcaagc gcaccggcag cctgatccag ggcgtggtgg acgcgaccac ctcgcgaagc
     5161 gaaggctggc attggggccg catcgcgcgg atgctcgagc gagcggacaa gacgtcgcgg
     5221 atcctggacg tcaaatactt catcttgctt cccaacgtga ccgacgtggg gacgccgctg
     5281 gacgacctgc agtggacggc gctgctgaaa tcggcgagtg cgctggagat gtatcgcaag
     5341 cgatacgggc gaatcgcccc ggaacgggtg gccgggttct tgcttctgga ccgggagttc
     5401 cctcgctcgg tgcagttctg cgtccacctg gccgatcttt cgctcagaag gattacggga
     5461 acacagagcg agaccttctc caacaaggcg gagcagatgc tcggcaggtt aggctccatg
     5521 ctcgcttact ccgggatcgg cgagatcgtc gcggcaggcc tgcacgaatt cttggatcag
     5581 ctacagaggg acctgaatac ggtggacgac gccatctacg aaggattctt cgcgctccgg
     5641 ccggccgggg tcgccagggg aggaaatcaa tgacgttctg cctcggaatg aaggttcgcg
     5701 acggcatcgt ggggatcgcc gacacgcgaa tcacctccgg cgccgaagcg atcacggcac
     5761 gaaagatttc gatccgtgag cacgaccggc actcactgtt catgatgacg tccggcctgc
     5821 ggtcggtccg cgacaaagcc ctgacctact tcgacgagat cgtggcgcgc gacgaaccgg
     5881 cgttcacgcg cctgtatcag gtcgcgaacg cgttcgcgga gcaggtccgg cgcgttgcga
     5941 aggaggacgg cgaggcgctc gccgaggccg gtctcgcgtt caacctgcac acgctcgtgg
     6001 gcggccagct cgatgaggac gccgaaccga tgctctacat gctctacccg cagggcaact
     6061 gggtcgaggt cacgcgcggc accccgtact tcatcatcgg cgagaccggc tacggcaagc
     6121 cgctgctcga ccgtgcgctc ggctacgacg cgccgctgga gcaggcgctc aagatcggct
     6181 atctcgcctt cgacgcgacc cggacgagcg cgaccgacgt ggacttcccc atcgacatcg
     6241 tggtgtaccg gaacgactcg ttctcgatgc gcgagcaccg ctacgagttc gaggagctga
     6301 gcgaggtgtc gcagatgtgg aaccgcaacc tgcgacggct catcgacgag gttccctcgc
     6361 gctggctgga ggccgtcttt ccttccgtga ccaagcccga caacgtccgc ccgatgccgg
     6421 gcgcatcccg cgagtcctga gacggcggcc gcatgcgctt ccgggttcgt cacctcaccc
     6481 gatacggata cgaccgcgcc gtgccgctcg gcgccctgac cgttcggctc cggccgcgtg
     6541 acgacggcac gcagcggctc gcgtcgtttt cgcttgcgtg cgacccggcg ccgtcgggtc
     6601 gctccgagat gctcgacgcc gaaggcatct ccgtcacgcg tttgtggttc gagggcgaga
     6661 cctcgtcctt gacggtcgag acctcgttcg aggccgagac gctgcgggcc aatccctacg
     6721 actgggtgat cgatcccggc atctgcgtgg cgccgatgcg ctacggcccg cgcgaggcgg
     6781 cggccctgac gcgcgagctt cgcttcggcg gcgatcccgc ggccgccgat ctttccgatg
     6841 agatcttgaa ggagtccgca ggatccgcgt ccgcgttcct gtcgctgctg tgttcgcgca
     6901 tcgccgccac cgttgtgcac gagcggcggc ccgaaggccc gcctcgcctc ccatccgaga
     6961 cgttgtcggc cggccacggc gcgtgccgcg acgttgccgt cacctacgtc gagagcgcgc
     7021 gcgcggcggg attcgccgcg cgattcgtca gcggctacac aacgcctgaa gacggatttc
     7081 ccggcgagct tcacgcctgg gccgaggtct acctgcccga cggggggtgg cgtggctacg
     7141 acgcgaccgg cggcattgcg gtcgccgaca cgcacgtcgc ggtcgcggcc gccgccgacc
     7201 cggccgcgac cgcgcccgtc gagggaacct acggcgccgg cgccgcgtcc gccctgaaaa
     7261 ccgtcgtcga catcgagacg agctgacccc ggatgcgacg cagggaggcc gccctgcctc
     7321 tcgacgaaag cggccggatc aggatgaaga atcggtgatc gcgatcagcc cgcccaggac
     7381 tccgacgtag gccgtgccgt ccggtccgag cgtcaccggc gcgtagttgt tgttgaagcc
     7441 gagcccgagc ccggtccgga ccttgtagac cgttgcgccc gtccggaagt ccagcgcggt
     7501 gaagtaccag gcgtccgagc cgtccggttg cggatccttc gtgtacgtgt agaccaggcc
     7561 gttggcgagc gagaccttcg gcacgaccga gggggcgcgc tcgttgctcg tccacgccac
     7621 ggtgcaggtg ccggcagacg ggtcgacgtc cacgcgcgca agccccggct cggtcacgcc
     7681 gccgctctgc gtcgcgagcg gacccgagta gccgtagttg ttctcggcga tcagcgagtt
     7741 gccgacggcg atgagcgact ggtcggtgtt cgacacaccg tcggcgaac
//
LOCUS       DATLFO010000129         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_62098, whole genome shotgun sequence.
ACCESSION   DATLFO010000129 DATLFO010000000
VERSION     DATLFO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62098"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..987)
                     /locus_tag="VM841_04770"
     CDS             complement(157..987)
                     /locus_tag="VM841_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL89530.1"
                     /translation="MKSRQQMVRVPTSHGEMALHISGTGSPVCFVHGIPGSAKSFERV
                     AARLEAGHTVVRADLLGFGASDRPSASASLLMSGQARALAEALDAVAVRRCAFVGHDY
                     GAVVILSLLAHDPARATRFAIAATNVFPDTPIPFPLSLVVHPALGSIAERAVFSTVSL
                     SLMLRMASGRGATPDRAAALGDAGQRRAIRMIFAEALRGLPTLYPPVRDALSRVAVPS
                     LVVWGDKDPFFDVEQGRRTADVLPHSRFVVYDGAGHFIPEERPDLLAADLLTLLAETE
                     "
     gene            1075..1725
                     /locus_tag="VM841_04775"
     CDS             1075..1725
                     /locus_tag="VM841_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL89531.1"
                     /translation="MAAYNQYCALAQALDRIGDRWTMLIVRELLVRPCRYSDLRDALP
                     GIATNLLAERLRRLETDGIVERRELGPPVATTVYGLTDLGRALEPAILSLIRWGGAWM
                     QRRRRGTGFRAHWLGLALRALLEPPPQFDAIVAIVTEGERITLHAKDGEIALRDDDVP
                     VAGELKAAPDVILGIAAGKIRLAQAERDGAAAITGDRRALGRLLRPPAPATQPLRI"
     gene            1799..2026
                     /locus_tag="VM841_04780"
     CDS             1799..2026
                     /locus_tag="VM841_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89532.1"
                     /translation="MESIVEFGRAKYDYDAEFAFALDLILDGLDDFSPPNRLAASRLG
                     KRTRPAHEVAGSGIELLDRGVKPLKGIRGEA"
     gene            complement(2076..3092)
                     /locus_tag="VM841_04785"
     CDS             complement(2076..3092)
                     /locus_tag="VM841_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006170410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVL89533.1"
                     /translation="MDEHVTPEPILQLGLAFWGSKTLLSAVELGLFTHLASGPCDAEK
                     VATDLDLHPRGVSDFLDALVSLGMLEREGDQYRNSAATDTFLDRNKPSYVGGLLEMAN
                     ARLYPFWGSLTEALRTGQPQNEAKVGENFFTALYQDPLKLEQFLRAMTGISMGDAHVI
                     AEKFPWDKYETVIDVGTSEGCVPVQLALRHPHLSGGGFDLPPVAPVFEKYVTSFGLSD
                     RLRFYPGDFFTDPLPSADVLVMGHILHDWSLDEKLMLLRKAFDALPAGGALIVYESII
                     DDSRRANAFGLLMSLNMLIETPEGFDYTAADCQSWMTDVGFRDTYTEPLVGPDSMVVG
                     RKPN"
     gene            3429..>3667
                     /locus_tag="VM841_04790"
     CDS             3429..>3667
                     /locus_tag="VM841_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89534.1"
                     /translation="MSLQLDGRLVERNDPDYESLRTSFNGLINRRPAEPAWVASLREH
                     GRAGLLYARLHGVMAALGIIFLVVFVSERVAPPGSP"
BASE COUNT          695 a         1202 c         1152 g          618 t
ORIGIN      
        1 cggagaaccc ttgcgtgcgc cgtcagtgtt tgcgcagacg atggaggttc tgctcgcgac
       61 gtattcggcc gtcttcttcg cagcgctggc cgccgctctg ggcgcgtact tcctcgaaca
      121 caagcacgaa accggatagc cgcccgaccg acgaagtcac tcggtctctg cgagcaatgt
      181 gagcagatcg gcggcaagca ggtcagggcg ttcctccggg atgaaatggc ccgctccgtc
      241 gtagaccacg aagcgcgaat ggggaaggac gtcggcggtt cgccggcctt gctcgacatc
      301 gaagaacggg tccttatccc cccacacgac cagcgatggc accgccaccc gcgaaagagc
      361 gtcccgcacg ggcggataca gggtcggcag cccgcgcagc gcttcggcga agatcatgcg
      421 gatcgcccgc cgttgaccgg catcgcccaa cgccgcggcc cggtcaggcg tggcgcctcg
      481 cccgctcgcc atccgaagca tcagcgaaag agagaccgtc gagaacacgg cacgctcggc
      541 gatcgatccc aaagccggat gaacgaccag cgacagaggg aacgggatcg gcgtgtccgg
      601 gaacacgttg gttgcggcga tcgcaaaccg ggtcgctctg gcagggtcat gtgcgagcag
      661 cgaaaggatc acgaccgcgc cgtagtcgtg gccgacgaac gcgcagcggc gcaccgcgac
      721 ggcgtcgagc gcctcggcga gcgctcgggc ttgtccgctc atgagcagcg aggccgatgc
      781 ggaaggacga tcgctcgcgc cgaagcccag caagtcggct cgtacgacgg tgtgcccggc
      841 ctcaaggcga gccgctaccc gctcgaagga cttcgcggag ccggggatgc cgtgaacgaa
      901 gcagaccggc gagccggtgc cggagatgtg cagcgccatc tcgccgtgcg acgtcgggac
      961 tcgaaccatc tgctgccgtg acttcatctc gatcagctcc gccgctcaaa ggttagacaa
     1021 gaaactaaag tactccgacg agaaaatcaa cttaatatcg gctatgctgg cgtcgtggcg
     1081 gcctacaacc agtactgcgc cctcgcccag gcccttgacc ggatcggcga ccggtggacg
     1141 atgctcatcg tgcgggagct gctcgtgcgg ccgtgccgct actccgacct tcgcgatgcg
     1201 ctgcccggga tcgcgaccaa tcttttggcg gaacggctcc ggcgcctcga aacggacggc
     1261 atcgtggagc gccgtgagct cggcccgccc gtggcgacga cggtctatgg attgacggat
     1321 ctcggccgcg cgctggagcc ggcgatcttg tccttgatcc gctggggagg agcgtggatg
     1381 cagcgacgcc gtcgcggcac gggattccgt gcgcactggc tcggactcgc gcttcgcgcg
     1441 ttgctcgaac ccccacccca gttcgacgcc atcgtcgcga tcgtgacgga aggcgaacgg
     1501 atcacgctgc acgccaagga cggcgagatc gccctgcgcg atgacgacgt gcccgtcgcg
     1561 ggagagctca aggcggcccc cgacgtgatc ctcggcatcg cggccggcaa gattcgcctc
     1621 gcgcaagcag aacgtgacgg cgccgccgca atcacaggcg accggcgcgc gctcggccgt
     1681 ctcctccgcc cgccggcacc ggccactcaa cccctgcgga tatgacgata ttcccttcga
     1741 cacgaccggt ttcgagggaa cgaatcctcg agagattcgc cgcgacgcac gatatctcgt
     1801 ggagtccatc gtggagttcg gccgcgcaaa gtacgactac gacgccgagt tcgcttttgc
     1861 cctggatctg atcctcgacg gcctagacga tttctcaccg cccaatcgtc tcgcggcctc
     1921 ccggctcgga aaaagaacgc gcccggcgca tgaggtcgcc ggttccggca tcgagctcct
     1981 cgaccgcggc gtgaaacccc tcaagggaat acgcggggaa gcctgattcg ttgatcgatg
     2041 aacgccggcc actcgaggat gccaccgtga ccccgctagt tcggcttccg tccgacgacc
     2101 atagaatcgg ggcccacgag tggctccgtg taggtgtccc ggaagccgac gtcggtcatc
     2161 caggactggc aatccgcagc tgtgtagtcg aagccctccg gtgtttcgat cagcatgttc
     2221 aggctcatga gcaacccgaa agcgttcgcc cggcgggaat cgtcgatgat gctctcgtag
     2281 acaatcagag cgcccccggc cggaagtgcg tcgaaagcct tgcgcagcag catcagtttc
     2341 tcgtcgaggc tccagtcgtg gaggatgtgg cccatcacga gcacgtccgc agacggcaac
     2401 ggatcggtaa agaaatcacc ggggtagaag cgcaggcggt ccgagaggcc gaacgacgtc
     2461 acatactttt cgaacaccgg agcgacgggt ggaagatcga acccaccgcc ggacaggtgg
     2521 gggtggcgaa gggcgagctg gacgggcacg cagccctcgg acgttccgac gtcgatgacg
     2581 gtttcgtact tgtcccacgg aaacttttcg gcgatgacgt gggcatcgcc catgctgatg
     2641 ccggtcatcg cacgaagaaa ttgctcaagc ttgagcgggt cttgatagag ggcagtgaag
     2701 aaattctctc ccaccttggc ctcgttctga ggctggcccg tacgcagcgc ctcggtcagc
     2761 gatccccaaa acgggtacag gcgcgcgttg gccatctcca agagcccgcc aacataggac
     2821 ggcttgtttc ggtcgaggaa tgtgtcggtc gcggcagagt tacgatactg gtcgccttcc
     2881 cgttccagca tcccgagcga gaccaaagca tcgagaaagt cgctcacacc ccttggatgc
     2941 aggtccagat ccgtcgctac cttctccgcg tcgcacggtc cactcgccag atgcgtgaaa
     3001 aggccgagct ccactgcgct caggagagtc ttcgatcccc aaaaggccag acccagttgg
     3061 aggatcggct caggcgtgac gtgctcatcc atgttttccc tttcaccagt gcacggccag
     3121 accgtacggt cctcgccatc ctttcacaaa cacatcgatg tccgcttgcc gatgtccatc
     3181 gcctcccgtg aggctggtca gaatccagcc cggccgagcc ggccacatgc gcggcgcccc
     3241 gatcactaca cgcagcacat accgggtctc ggagtgggcg agaaatgaat ggccgatcca
     3301 atggagcgag ttgcgtcatt ggaacatcag gctcgcgctg ctgtcgaacg tcccgacgcc
     3361 cccgacgtcg tcccttgact cgctctgata cttctgagct caggttgagt cttctcgcat
     3421 ggggcaacat gagccttcaa ctcgatggac gactggttga acggaacgat ccggactacg
     3481 agtccctccg cacttcgttc aacggactca tcaaccgcag gcctgccgag ccggcatggg
     3541 tggcatcgct gcgcgagcac ggtcgcgcgg gcctgctcta cgcccggctt catggggtca
     3601 tggcggctct cgggatcatc tttctcgtgg tgttcgtatc cgaacgcgtc gcaccgcccg
     3661 gaagccc
//
LOCUS       DATLFO010000130        16929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_54553, whole genome shotgun sequence.
ACCESSION   DATLFO010000130 DATLFO010000000
VERSION     DATLFO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54553"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM841_04795"
     CDS             <1..358
                     /locus_tag="VM841_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89535.1"
                     /translation="AMGIGVTVKNGTIWVTQDFIQERVPSRASFPKAPVVPRTSNRTS
                     VLPAGRPAPAAPPAATVAPIVPPKPKISALRLPPIPAPVAAPIDEQRRPLMPLAVLAT
                     LSAVAFASRKDAGAWI"
     gene            426..1496
                     /locus_tag="VM841_04800"
     CDS             426..1496
                     /locus_tag="VM841_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVL89536.1"
                     /translation="MDAMPRRAFLRGIGLAAAAAACSGNGSPRRVSSPSPAPTAPAGS
                     PTATATALPASWRRIEASGPSARRDATLTGDPARGAAYLFGGRSGSGTSLGDLWVYDV
                     AGGAWRKVDASEGPGPRFGHNAGMVGGKLVVFAGQGGPTTFFNDLYTFDPDVGRWSKG
                     AAGNAPAKRYGSGDAVVDDRLLISHGFTNSGRFDDTWTWSGSWSDASPGSGVRPVKRC
                     LHRSAWWPERNVVALFGGQTDGTPFLNDFWLYDPAAKTWTKAEPGTLPGPRTLFGMVA
                     LAGRLWVWSGFSPGGQVGDMWWIDDPAKPWQMQSFPDGQAPGPRGGIAAAAVSDGEAL
                     MFGGRVGDTDLDELWSFRPGPA"
     gene            complement(1493..2746)
                     /gene="tyrS"
                     /locus_tag="VM841_04805"
     CDS             complement(1493..2746)
                     /gene="tyrS"
                     /locus_tag="VM841_04805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVL89537.1"
                     /translation="MSDQPAEPLAPETAEQLKILTSGVDRVAPEHEFVRKLDRAIKTG
                     TGLRVKLGIDPSTPDIHIGHGVPLRLMRRFQQLGHTAVLIIGDFTGQVGDPTGRSETR
                     RALAADEVVANGRTYEAQARKILLPDRLEILRNSEWLGALGTAGLLQLASRMTVARML
                     ERDDFSNRYKSGQPISIVEFLYPLLQGHDSVVVRADVELGGTDQLFNLLVGRDLQVRD
                     GQEPQVAFTVPLLEGLDGEMKMSKSYGNYVGITDEPADMYGKLMSVPDRLLGKYLRLT
                     TDLHPDAIEQIESRAAGGGKAAADAKRRLAHEVTAIYHGTDAADAAAAHFDRIHVERA
                     IPGDIPEHDIPAESIQDGAVPLARLITALGFATGTSDARRKIEGRGVRVGGEVVEDPK
                     ATFDPAALAGKVIQVGKRSFAKIRG"
     gene            2854..5313
                     /locus_tag="VM841_04810"
     CDS             2854..5313
                     /locus_tag="VM841_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HVL89538.1"
                     /translation="MRSVLPLRGPRRVAAVFAVVSLATLTGCVQLPNLDDAMSAARRI
                     PETSFIYASDGALITRMHAEENRETIPLDQVPEVMRNAVIAIEDQRFYDHPGVDLRAI
                     IRAFFRNTNEGRVVEGGSTITQQYVKNVLVERTKTLKRKVTEASLAYQLERRHTKDEI
                     LERYLNTVYFGQGAYGIEAAAKTFFGVNARDLNLPQSALLAGLIKSPARYDPLVDGEG
                     AAARRDLVLREMMQLNMISPGEYDAALKTPLGVKPKQGAIRYEAGYFVEWVKGLIERD
                     PAFNVLGTSVAERVNALFKGGLRIHTTVDMRMQRIAEVASKEVLDRDKDPYNGFVAVD
                     PDTGAVKAMVGGRDFFDPDDPYAKFNLAVQSRRQPGSSFKPFTLAAALEKGIPLSRMY
                     RGGSQVSIRMPGGEVWSPKNYESLSFGSSLSVREATIKSVNVVYAQMIRDIGAEAVVE
                     MAKRLGITSELKPYLSLSLGAQEVSPLEMAVAYSAFANGGYRVHPHGITKITDAEGTV
                     LYEWRPTKTQVLKPRIVDQVNRALQDVIRYGTGRREQLPGRPAAGKTGTSDEYHDAWF
                     AGYVRQMVGVSWVGFPKAQIPMTPPYTRIRVVGGSWPGQIWKLFMVNALKDAPSLPFE
                     AAPEADMVTVRIDVTRNCRPNRFTPPNVIESVQFEKGTEPKEICTEPTAPVTATVPNV
                     IGRTEADARAVLEQAGFYAKVQQRACETYPPGVVCSQDPPGGTGGIAGTSVTIVVSND
                     TVRTSVPSVLGMARNAAISRLQDAGFDVDYVFAENGTTAPSGCQDGSVGGADRVWAQS
                     RCPGDEVPRGSRIVIYVNPIG"
     gene            complement(5310..5951)
                     /locus_tag="VM841_04815"
     CDS             complement(5310..5951)
                     /locus_tag="VM841_04815"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVL89539.1"
                     /translation="MSPAERARVSLRPVARTFFARPVLDVARDLIGCVLVHDTPEGRV
                     AGTIVETEAYGSDDPGSHAFRGPTPRNAPMFEAPARAYVYFTYGMHWCLNAVTDRTGV
                     PGAVLIRAVEPIEGLELMRRRRAAGAKAPARGIPDRDLARGPARLTQAFGVGPEHNRA
                     DLTRPPLFVGMGERFPDDAVRSTPRIGLGAAQDGRRWRFVVDGSRFTSGPRSL"
     gene            complement(5948..7678)
                     /gene="polX"
                     /locus_tag="VM841_04820"
     CDS             complement(5948..7678)
                     /gene="polX"
                     /locus_tag="VM841_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HVL89540.1"
                     /translation="MPRANDEAAALLQEMADLQAILGRDAFRVRAYEKAAAAVAAYAK
                     DIHGFDLTKLQGIPSVGKSMASRIQEFLDTGTISDLEALRLQVPAGVRAMTQIAGVGP
                     KRAIVLYREMGIDSVEALVEACKAEKLRGLKGFGAKTEEKILKSLELTSAHGDRVLID
                     TALDLAETLIAELRDMDEILDISYAGSLRRMRETIGDIDLLVASTNAAPIMERFTSLD
                     AAGHVLAHGDTKSAIVARGGLQVDLRVVEPDAWGAAMIYFTGSKAHNIKIRERAVKAG
                     LKLNEYGLFRLEDDERIAARTEDDVYEALGMAWIPPQIREDAGEIDAALNGTLPSLVQ
                     VKDLRGDLHTHTNMSDGHATLEEMVAEAARLGYSYYLVTDHAGDFMAMQRVTREKMLD
                     QRARIDTLQKKYPKLRILHGTELNIQPDGRVDYDPAFLAGFDLCVASVHSHFTLPREQ
                     QTARLLAAIENPNVHVIGHPSGRQIGRRAPIEFDEETVFKAAAASGTALEINAHPDRL
                     DLRDEHARLAKSLGATVSIDSDAHAVGHLAGIRFGVGTAQRGWIERGDVLNCKTLRQL
                     EAFVARKRAR"
     gene            complement(7690..9015)
                     /gene="argH"
                     /locus_tag="VM841_04825"
     CDS             complement(7690..9015)
                     /gene="argH"
                     /locus_tag="VM841_04825"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVL89541.1"
                     /translation="MTGGALWGGRFEGGLSPEMRALSRSTHFDVALVAHDCRVLAAHA
                     GALRTAGVLTAEEAAAIRDALPGIAADIAAGTFGIDEADEDIHSVVERALAERVPDAA
                     GKSRSGLSRNDRVATAFRLWIVDAGKRVSLLLDRLVGTLEERAIEHRDTIMPGYTHLQ
                     RAQPVTLAANLSAHAAAFRRDHDRITAALERAAVSPLGAGALAGSLLGLDRNAAAEEL
                     GLRAPIGNTLDAVASRDFALELLAVCAILGVHLSRLGEEIVLWTSSEFGFAVLDDAYA
                     TGSSLMPHKKNPDVAELARGKAGRLIGHLTGLLATVKSLPLSYNRDLQEDKEPVFDAI
                     ATLELVLPALAGAVATMRFDTTRLAEAATDPLLLATDLAEHLVARGVTFKDAHEIVGR
                     AVRRSVDEGRSWREWSGQDWTAVHPALADAGSLLDPAASVAKRSGSKAP"
     gene            complement(9012..10214)
                     /locus_tag="VM841_04830"
     CDS             complement(9012..10214)
                     /locus_tag="VM841_04830"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HVL89542.1"
                     /translation="MTDNICVLAYSGGLDTSIAIKWIKDNYGYDVIALAVDVGQPIDH
                     EAVISKGKASGAIDAMVVDARDEFAREYALPALKANALYMGKYPLVSALSRPLIAKHL
                     VRVARERGASAIAHGCTGKGNDQVRFEVSTTALAPDLRSIAPVRDFPMTREAAIAYAE
                     QHGIPIPVTRSSPYSIDENLWGRTIECGAIEDPWAAPPADAWVVTSNPAGSPDEPELL
                     TIEFESGTPVAIDGERLPLPDLILATGERAARHGIGRIDMIEDRLVGIKSRELYEVPA
                     ALTLIAAHRDLEDLCLERSLAAFKRSHVEPLYAELAYNGLWFGPLRAGLAAFVEESQA
                     HVTGEVRIRLHKGTAVVDGRRGPRALYTKHLATYDEGDTFDHTAAAGFVHLWGLPLKV
                     WSDVQGDR"
     gene            complement(10211..10717)
                     /gene="argR"
                     /locus_tag="VM841_04835"
     CDS             complement(10211..10717)
                     /gene="argR"
                     /locus_tag="VM841_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HVL89543.1"
                     /translation="MAARLSRADKQARQRRIVEILRRSDVESQGELARLLARGGDKVT
                     QATLSRDLEELGAVKVRAGDGRVVYRVPDEPPQPTDWLERMLREFLLEMDTSGNLCVL
                     KTPPGGANAVARALDNASVPDVLATIAGDDTILIVAKEGSTGKAVARRLRSLAGQAGG
                     AKGRDRSS"
     gene            complement(10702..11616)
                     /gene="argF"
                     /locus_tag="VM841_04840"
     CDS             complement(10702..11616)
                     /gene="argF"
                     /locus_tag="VM841_04840"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HVL89544.1"
                     /translation="MTVRHFVSMTSITPDEMRMLLDRAAALKDRRAPSQALAGKTVAM
                     IFEKPSTRTRVSFQVAIAELGAHPLALSAAELQLGRGETVADTGRVLGRYVHGIVVRT
                     FGQERVDELAATAGIPVVNALTDHEHPCQALADLLTIRERFGSLAGRSLAYVGDGNNV
                     AHSLLLAGAMAGMRVTVATPPGYEPDESVVDRATEIASATGGAIETGNDPVPLVSGAD
                     AVYTDVWASMGQEEEQAERVRAFAGFQVDEAMMSGAKPEAIALHCLPAHRGEEISAGV
                     LDGPQSEVWDQAENRLHVQKALLEWLLG"
     gene            complement(11613..12002)
                     /locus_tag="VM841_04845"
     CDS             complement(11613..12002)
                     /locus_tag="VM841_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL89545.1"
                     /translation="MSDHPLILVVEDDPSVVELERVLLEDEGYEVLTAKDGLEGLLKT
                     EFRRPALILLDLMLPVVDGARMFEELRADARVAATPVVIVTGMADAHNVFDDLVGREN
                     VIIKPFGLDELLARVVALAGPGRKDER"
     gene            complement(11999..12412)
                     /locus_tag="VM841_04850"
     CDS             complement(11999..12412)
                     /locus_tag="VM841_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVL89546.1"
                     /translation="MRERGAAKVWILAFVVFAAGGLGALIGIGCLISANACPWREEPK
                     QTSRDGATLFAANCAACHGRNGEGRNGPTLIDGPIASATLEELVNKISRGKPLAGMPR
                     FSRTLEVDQIRAVAEYVTTVRSDPSPDPSPSEPSP"
     gene            complement(12405..13601)
                     /locus_tag="VM841_04855"
     CDS             complement(12405..13601)
                     /locus_tag="VM841_04855"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HVL89547.1"
                     /translation="MTAFEAIRDRDGAYVMQTYGRQPVAFVRGDGARLWDSEGREYLD
                     FLAGIAVASVGHAHPAVTSAVRAQAGELVHVSNLFYTEAQGLLAERLHGLLGWGRSFF
                     CNSGAEANEAAIKLARRATGRAGLVTALGSFHGRTIATLAATGQPAKHEPFSPLPAEL
                     MNHIPFNDEEALEAAIGEDTSAVLLEVVQGEGGVHPATAEFLRAARKLCDERGALLIF
                     DEVQTGLGRTGSWFAFRHTGVEPDIVTLAKALGGGLPIGACIAREEVAAGFGPGDHAT
                     TFGGGPVPCRAALAVLDVIESGNLVQNAAAMGERLTERLTEATSGNDLVAGIRGLGLL
                     VGLQLTADRSAEAVERCLAGGLVVNNVTPSTIRLSPPLVVREAEVDRAAKILGDVLAG
                     MGSAGA"
     gene            complement(13598..14440)
                     /gene="argB"
                     /locus_tag="VM841_04860"
     CDS             complement(13598..14440)
                     /gene="argB"
                     /locus_tag="VM841_04860"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVL89548.1"
                     /translation="MTESLVQRARTLMEALPYIKRYHGRTIVVKYGGAAMDDPSLRPS
                     FASDVVLMRYVGMNPVVVHGGGPQVSEHIRRLGGEPRFAGGYRVTTAEDLMVARMVLC
                     GVINKDIVSLINRHGTLAAGVSGEDGRLLTVKPKSPELGFVGEVVRANPAILTDLLRD
                     FIPVVASLGVDEDGQAYNVNADEVAAALAAALGADKLVYLTNVEGILDGSGSLIRTLT
                     LDEAATAQASGAIGEGMIPKLQSAMDAVKGGVPKATILDGRVEHALLLEVFTDEGVGT
                     MVTP"
     gene            complement(14437..15603)
                     /gene="argJ"
                     /locus_tag="VM841_04865"
     CDS             complement(14437..15603)
                     /gene="argJ"
                     /locus_tag="VM841_04865"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HVL89549.1"
                     /translation="MTLPFGFTYAGVAAGLKPGGALDMGLIVSDRPAVAAGVVTANTF
                     QAAPIVVSKKHLRRGAARAIVVNAGNANACTGPLGIADAEAMAADTAAALGVGAEQVL
                     VASTGVIGRRMDMPKIRTGIAAAAAALGAEPAAFASAIMTTDTRAKIAEATAGDAAVY
                     GIAKGAGMIAPEMATMLCFILTDAPASRSVLAAALQRASAESFNRISVDGCMSTNDCI
                     FALANGTAGGEIIERGDPREAALADAIGQVCRSLARQIVEDGEGATKVVEITVRGATS
                     EREALVAARAIAGSVLLRCAIGGADPNWGRVLAALGTAPIPFDPHRVDVWLGGEKVAE
                     KGAIGPGDLDKAAAAMKDRDVEVLVDMRRGRHAATILTNDMTAEYVSINGDYTS"
     gene            complement(15600..16634)
                     /gene="argC"
                     /locus_tag="VM841_04870"
     CDS             complement(15600..16634)
                     /gene="argC"
                     /locus_tag="VM841_04870"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HVL89550.1"
                     /translation="MSYRAAVVGASGYSGAELLRLLAAHPSIEVAAATSREFAGKDIA
                     GVYPNLSAYAGRTFSSLEDAGLDQADIVFLALPHGASMSVGAPLADAGARIVDFSADF
                     RLTDAALYAAWYGADHAAPEWLGKWAYGLPEMHRDQIKGAALVANPGCYPTAAALALA
                     PLLAEGLIEPSGITIAAASGVSGAGRGMSAGVHFSHVDASVKAYGFGKHKHTPEIEQE
                     LSLAAGAPVTVTFVPHLVPTARGLLATCTARLVDGAGGARVRDAVVARYDGEPFVRAT
                     PAPPETKFVSGSNGAHVYATADERTGTAIAVCAIDNLGKGAAGQALQNANLMLGLDET
                     AGLTAGGIYP"
     gene            complement(16710..>16929)
                     /locus_tag="VM841_04875"
     CDS             complement(16710..>16929)
                     /locus_tag="VM841_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89551.1"
                     /translation="IASALAEAAGPLLASIDPFDVYRGGQVAEGKRSVAFALQFRDPE
                     RTLTDDEVDAAMSRAIDAAAVRGWAIRS"
BASE COUNT         2457 a         5828 c         6046 g         2598 t
ORIGIN      
        1 cgcgatgggc atcggcgtga ccgtcaagaa cggcaccatc tgggtcacgc aggacttcat
       61 tcaagagcgc gtgccgagcc gggcttcctt cccgaaggcc ccggtcgtgc cgagaacgtc
      121 gaaccgcacg tccgtgctgc cggctggccg gccggctccg gcggctcccc cggcggccac
      181 ggtcgccccg atcgttccgc cgaagccgaa aatctcggcg cttcgcctcc ccccgatccc
      241 ggcgccggtc gccgcgccga ttgatgagca gcgcaggccg ctcatgccgc tggccgtgct
      301 cgccacgctc tcggcggtcg ccttcgcgtc ccgcaaggac gccggcgcct ggatctgagc
      361 cggccccacg cagcggtgcg gccggccgcc ccccacccga tgatccggcg cgatatgctc
      421 gcaccatgga tgccatgccg cggcgggcct tcctgcgggg catcggcctg gccgcagctg
      481 cggctgcgtg cagcggtaac ggcagcccgc gcagggtctc ctcgccctct ccggccccga
      541 cggccccggc gggcagcccc accgcgaccg cgaccgcgtt gccggcgagc tggcggcgga
      601 tcgaggcatc aggtccgagc gcccggcggg acgcgacgct gaccggcgac cccgcgcgcg
      661 gggcggcgta tctgttcggc ggccggtccg gctccggcac gagcctcggc gacctgtggg
      721 tctacgacgt ggcgggcggg gcgtggcgaa aggtggacgc gagcgaagga cccggcccgc
      781 gcttcggtca caacgccggc atggtcggcg ggaagctcgt cgtcttcgcg gggcagggag
      841 ggccgacgac gttcttcaac gacctctata ccttcgaccc ggacgtcggc cggtggagca
      901 agggcgcggc gggcaacgcg ccggcgaagc gatacgggtc cggcgatgcg gtggtggacg
      961 accgtctgct catctcgcac ggcttcacca acagcgggcg gttcgacgac acctggacct
     1021 ggagcggctc gtggagcgat gcgagccccg gctccggagt gcggccggtc aagcggtgcc
     1081 tgcaccgctc ggcatggtgg ccggagcgca acgtcgtggc gctcttcggc ggccagaccg
     1141 acggaacccc gttcctgaac gacttctggc tctacgaccc ggccgccaag acctggacca
     1201 aggccgagcc gggcacgctg cccggaccac ggacgctgtt cgggatggtc gcgctggcgg
     1261 ggcggctgtg ggtgtggagc ggattttcgc ccggcggaca ggtcggcgac atgtggtgga
     1321 tcgacgatcc cgccaagcct tggcagatgc aaagcttccc cgatggccag gcgccgggcc
     1381 cgcgcggcgg catcgcggcc gcggcggtct cggacggcga ggcgctgatg ttcggcgggc
     1441 gggtcggaga caccgatctc gatgagctct ggtcgttccg cccggggccg gcctagcccc
     1501 ggatcttggc gaaggaccgc ttgccgacct ggatcacctt gccggccagc gccgccggat
     1561 cgaacgtcgc cttgggatct tcgacgacct cccctccgac gcgcacgcca cggccctcga
     1621 tcttgcggcg cgcgtccgag gttccggtcg caaaaccgag cgccgtgatc agccgagcga
     1681 gcggaaccgc gccgtcctgg atcgactcgg cggggatgtc gtgttcggga atgtcgccgg
     1741 ggatcgcgcg ctcgacgtgg atacggtcga agtgtgcggc ggcggcatcc gctgcgtcgg
     1801 tgccgtggta gatcgcggtc acctcgtggg cgaggcggcg cttggcgtcg gcggcggcct
     1861 ttccgccgcc cgcggcgcgg gactcgatct gctcgatcgc atcggggtgc aggtcggttg
     1921 tgagccgcag gtacttgccc agcagccggt cgggcacgct catcagcttg ccgtacatgt
     1981 cggcgggttc gtcggtgatg ccgacgtagt tgccgtagga cttcgacatc ttcatctcgc
     2041 cgtcgagacc ttcgagcagc ggcaccgtga acgcgacctg cggctcttgc ccgtcgcgca
     2101 cctgcaggtc gcggccgacg aggaggttga acagctgatc cgttccgccc agctccacgt
     2161 cggcgcgcac cacgaccgaa tcgtggcctt gcagcagcgg gtagaggaac tcgacgatcg
     2221 agatcggctg accgcttttg tatcggttgc tgaagtcgtc gcgctcgagc atgcgcgcga
     2281 ccgtcatgcg cgaggcgagc tgaagcaagc cggctgtgcc gagcgccccg agccactcgc
     2341 tgttgcgaag gatctccaag cgatcgggca gcaggatctt gcgggcttgg gcctcgtagg
     2401 tgcggccgtt ggcgacgacc tcgtcggcgg ccaacgccct gcgggtctcg ctgcggccgg
     2461 tcggatcgcc cacctggccg gtgaagtcgc cgatgatcag caccgccgtg tggccgagct
     2521 gctggaagcg acgcatcaag cgcagcggca cgccatgtcc gatgtggatg tccggcgtgg
     2581 acgggtcgat gccgagcttg acccgcagcc cggtcccggt cttgatcgcg cggtcgagct
     2641 tgcgaacgaa ctcgtgctcc ggcgcgaccc ggtcgacgcc ggaggtgagg atcttgagct
     2701 gctcggcggt ttcgggcgcg aggggctcgg caggctggtc gctcatggtg ggcgggatgc
     2761 taacacgctg gtcggggccg ttttcgcgcc tggccgcgcg gcccgggcga agccgatacc
     2821 atgggtcgcc tgccggtcgg tcggtgggag cagatgaggt cggtccttcc ccttcgcggg
     2881 ccgcgacgcg tcgcggccgt gtttgccgtc gtctcgctcg ccacgctcac gggctgcgtg
     2941 cagcttccca acctcgacga tgcgatgtcg gcggcgcggc ggattcccga gacctccttc
     3001 atctacgcgt ccgacggcgc gctgatcacc cggatgcacg ccgaagagaa ccgcgagacc
     3061 attccgttgg atcaggtgcc tgaggtcatg cggaacgcgg tgatcgcgat cgaggatcaa
     3121 cgcttctacg accatcccgg cgtggatctg cgggcgatca tccgcgcgtt cttccgcaac
     3181 accaacgagg gacgagtcgt cgaaggtgga tcgaccatca cgcagcagta cgtcaagaac
     3241 gtcttggtcg agcggaccaa gacgctcaag cgaaaggtca ccgaggcttc gctcgcctac
     3301 cagctcgagc gtcgccacac caaggacgag atcctggagc gttacctgaa caccgtctac
     3361 ttcggtcagg gcgcctacgg tatcgaggcg gcggccaaga ccttcttcgg ggtgaacgcg
     3421 cgcgatctga acctgccgca gtccgccctg ctggccgggt tgatcaagtc gcccgcgcgc
     3481 tacgacccgc tcgtggacgg cgagggcgcg gcagcacgac gcgatctggt gctgcgcgag
     3541 atgatgcagc tgaacatgat ctcgcccggc gaatacgatg ccgcgctcaa gacgccgctc
     3601 ggcgtcaagc ccaagcaagg cgccatccgg tacgaggccg gctacttcgt cgagtgggtg
     3661 aaaggcctga tcgagcggga cccggccttc aacgtgctcg gcacgtcggt tgccgaacgg
     3721 gtcaacgcgc tgttcaaggg cggtcttcgc atccacacca cggtggacat gcgcatgcag
     3781 cgcatcgcag aagtggcatc gaaggaggtg ctggaccgcg acaaggatcc ctacaacggg
     3841 tttgtcgcgg tggatccgga caccggcgcg gtcaaggcga tggtgggcgg ccgcgacttc
     3901 ttcgacccgg acgacccgta cgccaagttc aacctcgcgg tccagtcgcg ccgccagccg
     3961 ggctcgtcgt tcaagccgtt cacgctggcc gccgcgctcg agaagggcat tccgctgtcg
     4021 cgtatgtacc gcggcggctc gcaggtgagc atccggatgc cgggcggtga ggtctggagc
     4081 ccgaagaact acgagagcct gtcgttcggg tcgtcgcttt cggttcgcga ggcgacgatc
     4141 aagtccgtca acgtcgtcta cgcgcagatg atccgcgaca tcggcgcgga ggcggtggtc
     4201 gagatggcga agcggctcgg catcacgtcc gagttgaagc cgtacctgtc gctgtcgctg
     4261 ggcgcgcagg aggtcagccc gctcgagatg gcggtcgcgt actcggcctt cgccaacggt
     4321 gggtaccggg tccacccgca cggcatcacc aagatcaccg acgccgaggg cacggtcctg
     4381 tacgagtggc gtccgaccaa gacccaggtg ctcaagccgc ggatcgtcga tcaggtcaat
     4441 cgtgcgctgc aggacgtgat ccggtacggc acgggccgcc gcgagcagct tcccggacgg
     4501 ccggctgccg gtaagaccgg gacgtcggac gagtatcacg acgcgtggtt cgcgggatac
     4561 gtgcggcaga tggtcggcgt gtcgtgggtc gggttcccca aggcacagat tccgatgacc
     4621 cctccgtaca cgcgcatccg cgtcgtcggc ggatcgtggc ccggccagat ctggaagctg
     4681 ttcatggtca acgccctcaa ggacgcaccg tcgttgccgt tcgaggccgc ccccgaggcc
     4741 gacatggtga cggtccgcat cgatgtgacg cgcaactgcc ggccgaaccg gttcacgccg
     4801 ccgaacgtca tcgagagcgt ccagttcgaa aagggaaccg agccgaagga gatctgcacc
     4861 gagccgaccg cgccggtgac ggccacagtt cccaacgtca tcgggcgtac cgaggccgac
     4921 gcccgggcgg tgctcgagca ggccggcttc tacgcgaagg tgcagcagcg cgcgtgcgag
     4981 acgtatccac cgggggtcgt gtgctcgcag gatccaccgg gcggcaccgg cggaatcgcc
     5041 ggaacgtcgg tgacgatcgt ggtgtccaac gacaccgtgc gtacgtcggt gccgagcgtg
     5101 ctcggcatgg cgcgaaacgc cgcgatctcg cggctgcaag acgccggctt cgacgtggat
     5161 tacgtcttcg ccgaaaacgg cacgaccgcg ccgagcggct gccaggacgg cagcgtcggc
     5221 ggcgcggatc gagtgtgggc gcagtcgcga tgtccgggcg acgaggtgcc tcgcggcagc
     5281 cgcatcgtca tctacgtgaa cccgatcggc tagaggcttc gcgggccgct ggtgaagcgg
     5341 cttccgtcca cgacgaagcg ccagcggcgg ccgtcctggg ccgcgccgag cccgatgcgc
     5401 ggcgtcgagc gcacggcgtc gtccgggaag cgctccccca tcccgacgaa cagcggcggc
     5461 cgggtcaggt cggcgcggtt gtgctcgggc ccgacgccga acgcctgggt caggcgtgcg
     5521 ggcccgcgcg ccagatcgcg atccgggatg ccgcgcgcgg gggccttggc gccggccgcc
     5581 cggcggcgac gcatgagctc gagcccttcg atcggctcga cggcgcggat cagcactgcg
     5641 cccggcacgc cggtccggtc cgtgacggcg ttgagacacc aatgcatgcc gtaggtgaag
     5701 tagacgtagg cgcgcgcggg cgcctcgaac atcggggcat tgcgcggggt gggacctcga
     5761 aacgcatggc tgcccggatc gtcggagccg taggcctcgg tctccacgat cgttccggcc
     5821 acgcggccct ccggcgtgtc gtggaccagg acgcagccga tcaggtcgcg tgcgacgtcg
     5881 aggacggggc gtgcgaaaaa ggttcgcgcc accggccgca gcgacacgcg cgcacgctcc
     5941 gccggactca tcgcgcacgt ttgcgggcga cgaacgcctc gagctgcctg agggtcttgc
     6001 agttgagcac gtcgcccctc tcgatccaac cgcgctgcgc ggtcccaacc ccgaaccgga
     6061 tgcccgcgag gtgaccgacc gcgtgcgcgt ccgagtcgat cgacacggtc gcgccgaggc
     6121 tcttggcgag ccgcgcgtgc tcgtcgcgaa ggtccagccg gtccgggtgc gcgttgatct
     6181 cgagcgcggt tccggacgcg gcggcggcct tgaagaccgt ctcctcgtcg aactcgatcg
     6241 gcgcgcgccg gccgatctgc cgtccgctcg gatgcccgat gacgtgaacg ttcggattct
     6301 cgatcgcggc gagcaggcgg gcggtttgct gctcgcgcgg cagcgtgaaa tgcgagtgga
     6361 cgctcgccac gcacaggtcg aagcccgcca ggaacgcggg gtcgtagtcg acgcgtccgt
     6421 ccggctggat gttgagctcg gtgccgtgca ggatgcgcag tttcggatac ttcttctgca
     6481 gcgtgtcgat gcgcgcccgc tggtcgagca tcttctcgcg cgtcacgcgc tgcatcgcca
     6541 tgaagtcgcc cgcgtgatcg gtcaccaggt agtaggagta gcccagacgc gcggcctcgg
     6601 ccaccatctc ctcgagcgtc gcgtgcccgt cggacatgtt ggtgtgcgtg tgcaggtcgc
     6661 cgcgaaggtc tttcacctgc accaggctcg gcagggtccc gttcaaggcg gcgtcgatct
     6721 cgccggcgtc ctcgcggatc tgcggcggga tccaggccat gccgagagcc tcgtagacgt
     6781 cgtcctcggt gcgcgccgcg atccgctcgt cgtcctcgag gcggaacagc ccgtactcgt
     6841 tgagcttgag tccggccttg accgcgcgct cgcgaatctt gatgttatgc gccttcgagc
     6901 cggtgaaata gatcatcgca gcgccccacg cgtcgggttc gacgacgcga agatcgactt
     6961 gaaggccgcc gcgcgcgacg atggccgact tggtgtcgcc gtgggcgagc acgtgtccgg
     7021 cggcatccag gctcgtgaag cgctccatga tcggcgcggc gttcgtcgag gcaacgagca
     7081 ggtcgatgtc gccgatggtc tcacgcatgc ggcgcagcga gccggcgtag gagatgtcga
     7141 ggatctcgtc catgtcccgc agttcggcga tcaaggtctc ggccaggtcg agtgcggtgt
     7201 cgatgagtac gcggtcgccg tgcgcgctgg tgagctccag gctcttgagg atcttctcct
     7261 cggtcttggc gccgaagcct ttcagcccgc gcagcttctc ggccttgcac gcctcgacga
     7321 gcgcctcgac gctgtcgatg cccatctcgc gatacagcac gatcgcgcgc ttcgggccga
     7381 cgccggcgat ctgggtcatc gcgcgcacgc cggccgggac ctgcagccgc agcgcttcca
     7441 ggtccgagat cgtcccggtg tcgaggaact cctggatgcg ggacgccatg ctcttgccga
     7501 cggacggaat cccttgcagc ttggtcaggt cgaagccgtg gatgtccttg gcgtaggcag
     7561 cgacggccgc ggcggccttc tcgtaggcgc gcacccggaa cgcatcgcgg ccgaggatgg
     7621 cctggaggtc ggccatctct tgcagcagcg cagcggcttc gtcgttcgcc cggggcatcg
     7681 ggcggaattc tacggggctt tcgagcctga tcgcttcgcg accgatgcgg cgggatcgag
     7741 cagcgaaccg gcgtcggcga gcgcggggtg cacggcagtc cagtcctggc cgctccactc
     7801 gcgccaggac cggccctcgt cgaccgaccg gcgcacggcc cggcccacga tctcgtgcgc
     7861 gtccttgaac gtgaccccgc gcgccacgag atgctcggcg agatcggtcg ccagcagcag
     7921 cggatcggtc gcggcctcgg ccagccgggt ggtgtcgaag cgcatcgttg cgacggcgcc
     7981 ggcgagcgcg ggaagcacga gctcgagcgt ggcgatcgcg tcgaagacgg gttccttgtc
     8041 ttcttgcagg tcgcggttgt aggacagcgg aagcgacttc accgtggcga gcaggcccgt
     8101 gaggtgtccg atgagccggc cggccttgcc gcgcgcgagt tcggcgacgt ccgggttctt
     8161 cttgtggggc atgaggctgg acccggtcgc gtaggcgtcg tcgaggacgg cgaagccgaa
     8221 ctcgctcgag gtccacagca cgatctcctc gccgagacgt gacagatgca cgccgaggat
     8281 cgcgcacacg gcgaggagct cgagcgcgaa gtcgcgggat gcgacggcat cgagcgtgtt
     8341 gccgatcggg gcgcgcaggc cgagctcctc ggccgcggcg ttccggtcca ggcccagcag
     8401 cgagccggcg agcgcgcccg cgccgagtgg cgagaccgcg gcgcgctcca gcgcggccgt
     8461 gatgcggtcg tggtcgcgcc ggaacgccgc cgcgtgcgcc gacaggttcg cggcgagcgt
     8521 gacgggctgg gcgcgctgca agtgggtgta gccgggcatg atcgtgtcgc gatgctcgat
     8581 cgcgcgctcc tcgagcgtgc cgacgagccg gtcgagcagc aaggaaacgc gtttgccggc
     8641 gtccacgatc cacagccgga acgcggttgc gacccggtcg ttgcgcgaca ggcccgaccg
     8701 cgactttccg gcggcatccg ggacgcgctc ggcgagcgcg cgctcgacga ccgagtggat
     8761 gtcctcgtcg gcctcgtcga taccgaaggt gccggctgcg atgtcggcgg cgatgcccgg
     8821 gagcgcgtcg cggatcgcgg cggcctcctc ggcggtgagc acgcccgccg tccggagcgc
     8881 gcccgcatgc gcggcgagaa cccggcagtc gtgggcgacg agcgcgacgt cgaagtgcgt
     8941 gctgcgcgac agcgcgcgca tctccggtga cagcccgcct tcgaagcggc cgccccacag
     9001 cgcgccgccg gtcaccggtc tccttgcacg tccgaccaga ccttgagcgg aaggccccac
     9061 aggtgaacga acccggccgc cgcggtgtga tcgaacgtgt cgccttcgtc gtaggtggcg
     9121 aggtgcttgg tgtagagcgc cctggggccg cgccggccgt ccacgaccgc ggttcctttg
     9181 tgcagtcgaa tgcgcacctc gccggtcacg tgcgcttgcg actcctcgac gaatgcggcg
     9241 aggccggcgc gaagcggccc gaaccacaga ccgttgtagg cgagctcggc gtagagcggc
     9301 tcgacgtggg agcgcttgaa cgcggccagc gatcgctcca ggcacaggtc ttccaggtcg
     9361 cggtgcgcgg cgatcagggt gagcgcggcc ggcacctcgt agagctcgcg gctcttgatg
     9421 ccgaccagcc ggtcttcgat catgtcgatg cggccgatgc cgtgacgcgc cgcgcgctcg
     9481 ccggtcgcca ggatcaggtc cggaagcggc aggcgctcgc cgtcgatcgc gacgggggtg
     9541 ccggactcga actcgatcgt cagaagctcc ggctcgtcgg gcgagccggc cgggttcgag
     9601 gtgaccaccc acgcgtcggc gggtggtgcg gcccacggat cctcgatcgc gccgcactcg
     9661 atggtccggc cccacaggtt ctcgtcgatc gagtagggcg aggagcgggt gaccgggatc
     9721 gggatcccgt gctgctccgc gtaggcgatg gcagcctccc gcgtcatcgg gaagtcgcgt
     9781 accggcgcga tcgagcgaag gtcgggcgcc aatgcggtgg tcgagacctc gaaacgaacc
     9841 tgatcgttgc ccttgccggt gcagccgtgc gcgatcgcgc tcgcgccgcg ctcgcgggca
     9901 acccggacca aatgcttggc gatcagcgga cgcgacaggg ccgacaccag cggatacttg
     9961 cccatgtaga gcgcgttcgc cttgagcgcc ggcagcgcgt actcgcgtgc gaactcgtcg
    10021 cgcgcgtcca ccaccatcgc atcgatcgct cccgacgcct tgcccttcga gatcaccgct
    10081 tcgtgatcga tcggctggcc gacgtccacc gcgagcgcga tgacgtcgta gccgtagtta
    10141 tccttgatcc atttgatcgc gatgctcgtg tcgagcccgc ccgagtaggc gagcacgcag
    10201 atgttgtcgg tcatgacgat cggtcccttc ctttggctcc gcccgcttgg ccggcgagcg
    10261 agcgcaagcg ccgcgccacg gccttcccgg tgcttccttc cttcgcgacg atcaggatcg
    10321 tgtcgtcgcc ggcgatcgta gcgagcacgt ccggcaccga cgcgttgtcg agcgcgcgcg
    10381 ccacggcgtt cgcgccgccc ggaggtgtct tgagcacgca taggttcccc gacgtgtcca
    10441 tctcgaggag gaactcgcgc agcatgcgct cgagccagtc cgtcggttgc ggcggctcgt
    10501 cgggcacgcg gtagacgacg cgcccgtcgc cggcgcgcac cttgaccgcc ccgagctctt
    10561 cgaggtcgcg gctcaaggtc gcctgcgtca ctttgtctcc gccgcgggcc agcagacgcg
    10621 cgagctcgcc ttggctctcg acgtcgctgc gccgcagaat ctcgacgatt ctgcgctgtc
    10681 gcgcctgctt gtcggcgcgg ctcagccgag cagccattcc agcaacgcct tctgaacgtg
    10741 cagccggttc tccgcctgat cccagacctc ggactgcggg ccgtcgagta ccccggccga
    10801 gatctcttcg ccgcggtggg ccggcaggca atgcagcgcg atggcttcgg gcttcgcgcc
    10861 ggacatcatc gcctcgtcca cttggaagcc cgcgaacgcg cgtacccgct cggcctgctc
    10921 ctcctcttgc cccatcgagg cccacacgtc cgtatagacg gcgtccgcgc ccgacaccag
    10981 cggcaccgga tcgttcccgg tctcgatcgc gccgcccgtc gccgacgcga tctcagtcgc
    11041 gcggtcgacg acgctctcgt ccggctcgta gccgggcggg gtcgcgacgg tgacgcgcat
    11101 gccggccatc gccccggcga gcaggaggga atgcgcgacg ttgttgccat ccccgacgta
    11161 ggcgagcgag cggcccgcca gcgacccgaa tcgctcccgg atggtgagca ggtcggcaag
    11221 cgcctggcag ggatgctcgt ggtcggtcag cgcattcacg accgggatgc ccgcggtggc
    11281 cgcaagctcg tccacgcgct cctggccgaa cgtgcgcacg acgattccgt ggacgtagcg
    11341 gcccagcacc cgcccggtgt cggccacggt ctcgccgcgg ccgagctgaa gttccgcggc
    11401 ggacagggcg agcggatgag cgccgagctc ggcgatggcg acctggaacg agacgcgcgt
    11461 gcgggtcgag ggcttttcga agatcatcgc aacggtcttt ccggctagcg cctgcgacgg
    11521 cgcgcgccgg tccttcagcg ccgccgcacg gtccagcagc atgcgcatct cgtccggggt
    11581 gatcgaggtc atggacacga agtgccgaac cgtcatcgct cgtcctttcg gccgggcccg
    11641 gcgagcgcca ccacgcgggc gagcagctcg tcgaggccga acggcttgat gatcacgttc
    11701 tcgcgtccga ccagatcgtc gaacacgttg tgcgcgtcgg ccatgccggt cacgatcacc
    11761 accggcgttg ccgcaacccg cgcgtcggcg cgcagctcct cgaacattcg cgcaccgtcc
    11821 acgaccggca acatcaggtc gagcaggatc aacgccggac ggcggaactc ggtcttgagc
    11881 agaccttcga gcccgtcctt ggcggtgagc acctcgtagc cttcgtcttc gagcaggacg
    11941 cgttcgagct cgaccacgga cggatcgtcc tcgacgacca ggatcagtgg gtgatcgctc
    12001 acggtgaggg ctccgaggga gacggatccg gcgacgggtc gctgcgcacg gttgtgacgt
    12061 actcggccac tgcacggatc tgatcgactt cgagcgtgcg cgagaagcgg ggcatgcccg
    12121 cgagcggctt gccgcgcgag atcttgttga cgagctcctc cagcgtcgcc gaggcgatgg
    12181 gcccgtcgat caacgtcgga ccattgcgac cctcgccgtt gcgtccgtgg caggccgcgc
    12241 agttggcggc gaagagcgtc gcgccgtcac gggaggtttg cttgggctcc tcgcgccacg
    12301 gacaggcgtt cgccgagatc aggcagccga tgccgatcag ggccccgagg cccccggcgg
    12361 caaagacgac gaacgccagg atccatacct tggccgcgcc gcgctcacgc accggcggat
    12421 cccatcccgg cgagcacgtc gcccagaatc ttcgccgcgc ggtccacctc ggcctcgcgc
    12481 acgacgaggg gcggggacaa gcggatcgtc gagggcgtga cgttgttgac cacaagccct
    12541 ccggcgaggc agcgctcgac ggcttcggcg ctgcggtccg cggtcagctg cagcccgacg
    12601 agcaggccga gaccgcggat gccggcgacc agatcgttgc cgctcgtcgc ttcggtgagc
    12661 ctttcggtca gacgctcgcc catggcggcc gcattctgca cgagattccc ggactcgatc
    12721 acatcgagga ccgcgagcgc ggcgcggcag ggcaccggcc cgccgccgaa cgtggtggcg
    12781 tggtcgccgg ggccgaagcc ggccgcgacc tcctcgcgcg cgatgcacgc accgatggga
    12841 agcccgccgc cgagcgcctt ggcgagcgtg acgatgtcgg gctcgacgcc ggtgtgccgg
    12901 aaggcgaacc acgatccggt gcggccgagg ccggtttgaa cctcgtcgaa gatcagcagg
    12961 gcgccgcgct catcgcacag cttgcgggcc gcgcgcagga actcggcggt cgccggatgc
    13021 acgccccctt cgccctggac gacctcgagc agcaccgccg aggtgtcctc gccgatggcc
    13081 gcctcgagcg cttcctcgtc gttgaacggg atgtggttca tcagctcggc gggcagcggc
    13141 gagaacggct cgtgcttggc gggctggccc gtggcggcga gcgtggcgat ggtgcgtccg
    13201 tggaacgagc cgagcgcggt caccaggccg gcgcggccgg tggcgcggcg ggcgagcttg
    13261 atcgcggcct cgttggcttc ggcgccggag ttgcagaaga acgagcggcc ccagccgagc
    13321 aggccgtgca gccgttccgc gagcaggcct tgcgcctcgg tgtagaacag gttcgacacg
    13381 tgcacgagct cgccggcctg cgcgcgaacc gccgacgtta ccgccggatg cgcgtgcccg
    13441 acgctcgcga cggcgattcc ggccaggaaa tcgagatact cgcggccctc gctgtcccac
    13501 agccgcgcgc cgtcgccccg aacgaacgcc accggctggc ggccgtaggt ctgcatcacg
    13561 tacgcgccgt cgcgatcccg gatggcctcg aacgcggtca cggcgtcacc atcgttccga
    13621 ctccttcgtc cgtgaacact tccagcagca gcgcgtgctc cacgcggccg tcgaggatcg
    13681 tggccttcgg aacgcccccc ttgaccgcgt ccatcgcgct ttgaagcttg ggaatcatgc
    13741 cttctccgat cgcgcccgac gcttgcgccg tcgcggcctc gtccaaggtc agcgtgcgga
    13801 tcagcgatcc gctgccgtcg aggattcctt cgacgttggt gaggtagacc agcttgtccg
    13861 cgccgagcgc ggccgccagg gcggcggcga cctcgtcggc gttgacgttg taggcctggc
    13921 cgtcctcgtc gacgccgagc gaggccacga ccgggatgaa gtcgcgaagc aggtcggtca
    13981 ggatcgcggg gttcgcgcgc acgacctcgc cgacgaaccc cagctccgga cttttgggct
    14041 tgaccgtcag gagccggccg tcctcgccgc tgaccccggc ggcgagcgtg ccgtgccggt
    14101 tgatgagcga gacgatgtcc ttgttgatca cgccgcacag caccatgcgc gccaccatca
    14161 ggtcctcggc ggtggtcacc cggtagccgc cggcgaagcg gggttcgccg ccgagccggc
    14221 ggatgtgctc ggagacctgc ggccccccgc cgtgcaccac gacggggttc atgcccacgt
    14281 atcgcatcag aaccacgtcg ctggcgaacg acgggcgcag cgacgggtcg tccatggccg
    14341 cgccgccgta cttcacgacg atagtccggc cgtggtagcg cttgatgtag ggcagagcct
    14401 ccatcaaggt ccgggcgcgt tgaacgaggc tttcggtcac gacgtgtagt ccccgttgat
    14461 cgagacgtac tcggcggtca tgtcgttggt caggatggtc gccgcgtgac ggccgcggcg
    14521 catgtccacg agcacctcga cgtcgcggtc cttcatcgcg gccgcggcct tgtcgaggtc
    14581 gccgggaccg atcgcgccct tctcggcgac cttctcgccg ccgagccaca cgtcgactcg
    14641 atgcggatcg aacgggatcg gcgcggtgcc gagggcggcg agcacccggc cccagttcgg
    14701 gtccgccccg ccgatcgcgc agcgcagcag cactgagccg gcgatcgcgc gcgccgcgac
    14761 cagcgcctcg cgctcgctgg tcgcgccccg aacggtgatc tcgacgacct tcgtcgcgcc
    14821 ctcgccgtcc tcgacgatct ggcgagccag ggaccggcag acctggccga tcgcgtcggc
    14881 gagcgcggcc tcgcgcgggt cgccgcgctc gatgatctcg ccgcccgcgg taccgttggc
    14941 gagagcgaag atgcaatcgt tggtggacat gcagccgtcg acgctgatgc ggttgaacga
    15001 ctccgccgat gcgcgctgga gggcggcggc gagcaccgat cgtgaggcgg gcgcgtcggt
    15061 gaggatgaag cacagcatcg tcgccatctc gggggcgatc attccggcgc ccttggcgat
    15121 gccgtagacg gccgcgtcac ccgcggtggc ttccgcgatc ttggcccgcg tgtcggtcgt
    15181 catgatcgcg gatgcgaatg cggcgggctc ggcgccgagg gccgcggccg cggcggcgat
    15241 gcccgtccgg atcttgggca tgtccatgcg gcggccgatc acgccggtgg aggcgaccaa
    15301 aacctgctcg gcgccgacgc ccagcgcggc ggccgtgtcg gcggccatgg cctcggcgtc
    15361 ggcgatgccg agggggccgg tgcatgcgtt ggcgttgccg gcgttgacga cgatcgcgcg
    15421 ggcggcgccg cggcgcagat gcttcttgga aaccacgatg ggtgcggcct gaaacgtgtt
    15481 cgccgtgacc acgccggcgg ccaccgccgg tcggtcgctc acgatcaggc ccatgtcgag
    15541 cgcgccgccc ggcttgagcc cggcggcgac gcccgcgtag gtgaagccaa acgggagcgt
    15601 catggataga tcccccctgc ggtcaagccc gcggtctcgt ccagaccgag catcaggttg
    15661 gcgttctgca gcgcctgccc ggccgctcct tttccgaggt tgtcgatcgc gcagaccgcg
    15721 atggccgtgc cggtgcgctc gtcggccgtc gcatacacgt gcgcgccgtt ggagcccgac
    15781 acgaacttcg tctcgggcgg cgccggcgtt gcacgcacga acggctcgcc gtcgtagcgc
    15841 gcgacgaccg cgtcccgcac ccgcgccccg ccggcgccgt ccaccagccg ggcggtgcag
    15901 gtcgcgagca ggccgcgcgc ggtgggaacc aggtgcggta cgaacgtcac cgtaaccggc
    15961 gcgccggcgg cgagggaaag ctcctgctcg atctcgggcg tgtgcttgtg cttgccgaag
    16021 ccgtacgctt tgacgctggc gtccacgtgc gagaaatgaa cgccggcgct catgccgcgg
    16081 cccgcgcccg acacgccgga ggccgcggcg atcgtgatgc ccgacggctc gatcaggccc
    16141 tcggccagca gcggcgcgag cgcgagcgct gccgcagtcg gatagcagcc ggggttggcg
    16201 accagcgccg cgcccttgat ctgatcgcga tgcatctccg gcaggccgta ggcccacttt
    16261 ccgagccact ccggcgcggc gtgatcggcg ccgtaccagg cggcgtagag cgccgcgtcg
    16321 gtcaggcgga agtcggcgga gaaatccacg atgcgcgctc ccgcgtcggc gagtggcgca
    16381 ccgacgctca tcgaggcgcc gtgcgggagg gcgaggaaca cgatatcggc ctggtcgagc
    16441 ccggcatcct cgagcgagct gaacgtgcgc ccggcgtagg cggataggtt gggatagacg
    16501 ccggcgatgt ccttgcccgc gaactcgcgc gaggtcgcgg ccgcgacctc gatcgacgga
    16561 tgtgcggcga gcaggcggag cagctcggct ccgctatacc ccgaggcacc cacgacggcg
    16621 gcacgatagg acacgacgac ccctccacgg cttcaagtcg catgagaata caggtgtgtg
    16681 catacctatg caaccgggcg gacggccggt caggagcgga tcgcccagcc gcggacggcg
    16741 gccgcgtcga tggcccggct catcgcggca tcgacctcgt cgtcggtcag ggtccgttcc
    16801 gggtcgcgga actgcagggc gaaggcgacg ctgcgcttgc cctcggccac ctgcccgccc
    16861 cggtagacgt cgaagggatc gatcgaggcg agcagcggcc ccgccgcctc ggccagcgcc
    16921 gaggcgatc
//
LOCUS       DATLFO010000131         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_2842, whole genome shotgun sequence.
ACCESSION   DATLFO010000131 DATLFO010000000
VERSION     DATLFO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2842"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VM841_04880"
     CDS             complement(<1..196)
                     /locus_tag="VM841_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89552.1"
                     /translation="MRLAADGLHVRAEQIEAGDLLPYWQPGGFFFEREGEGIVAFGAA
                     ATITVPPSATQVADAAARVSE"
     gene            complement(193..894)
                     /locus_tag="VM841_04885"
     CDS             complement(193..894)
                     /locus_tag="VM841_04885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HVL89553.1"
                     /translation="MARRVKPLPAGEEKARAVRGMFDAIAPRYDTVNRIMTFGMDRGW
                     RRRAAATLGLDRGATVLDIACGTGDFCVEIERRGWRAIGLDFAGEMLARAKERGVPRL
                     VHGDALALPVADGSVRGITCGFALRNVSDLGRLFDEMARVLEPGGRIALLETAEPRNV
                     VLRAGHAVYFKRMVPLIGGLLSNRDAYAYLPRSIVYLPDPAEMLGMLERAGFTEVSRI
                     APGGGIVQILVGTRA"
     gene            complement(879..1655)
                     /locus_tag="VM841_04890"
     CDS             complement(879..1655)
                     /locus_tag="VM841_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVL89554.1"
                     /translation="MEYRVEQLAAACDVSVDTVRFYQAKGLLTPPRRSGRVALYSSEH
                     AERIRRIRSLQKKGLTLQAIERVVSGRLGRADQDLAAAVASAQAEGDAEEFISLEELA
                     ERSGVPAPLLASIEREGLPIGREIDGETRYAASDVELVRTGLRLLSAGLPLGELIALA
                     RAQNDATRAIAEEAVRLFDEYVRTPIKRSASSDEEAAEKLVAAFGELLPAVTALVAHH
                     FRRVLLQVAEEHIEKTGDAAEIAATREQSRRRLEAAWPGE"
     gene            complement(1710..>2325)
                     /locus_tag="VM841_04895"
     CDS             complement(1710..>2325)
                     /locus_tag="VM841_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL89555.1"
                     /translation="VTGITYRHPSLLATEAVTVDHISNGRLELAVGAAWHEPEHRRLG
                     LEFPPAGERVDRLEEAVQVMRALMTEDAPRFEGRHFRLEGGYNPKPVQQPHPPIWIGG
                     HGPRMVALAARHADVWHTFGRPDDLKQRSEMLSRHAETAGRDPSSIVRSTALSLSQTM
                     AEVRADAQAKADAGFTYLTIGWPSEGRERLDEFIGAAMADVAAM"
BASE COUNT          329 a          828 c          833 g          335 t
ORIGIN      
        1 tctcgctcac gcgtgccgcc gcgtcggcga cctgcgtggc cgacggaggg acggtgatcg
       61 tggccgcggc gccgaacgcg acgatgcctt cgccttcgcg ctcgaagaag aatccgccgg
      121 gctgccagta cggaagcagg tcgcccgcct cgatctgctc ggcgcgaacg tgcagcccgt
      181 ccgccgcgag cctcacgcgc gtgtcccgac caggatctgc acgatgccgc cgccgggcgc
      241 gatgcgcgag acctcggtga agcccgcgcg ctcgagcatg ccgagcatct cggcgggatc
      301 gggcaggtag acgatcgagc gtggcaggta cgcgtaggcg tcgcggttcg acagcagtcc
      361 gccgatcagc ggaaccatgc gcttgaagta cacggcgtga ccggcgcgca gcacaacgtt
      421 gcgcggctcg gcggtctcga gcagcgcgat gcggccgccc ggctcgagca cgcgcgccat
      481 ctcatcgaac agccgtccaa ggtcgctgac gttgcggagc gcgaacccgc aggtgatgcc
      541 gcgcaccgag ccgtcggcga cgggcagcgc gagcgcgtcg ccgtgcacga gccgtggcac
      601 gccgcgttcc ttggcgcgcg cgagcatctc gccggcgaag tcgagcccga tcgcgcgcca
      661 cccgcggcgt tcgatctcga cgcagaagtc gccggtgccg caggcaatgt cgagcacggt
      721 tgcgccccgg tccagcccca gcgtcgcggc ggcgcggcgg cgccagccgc gatccatgcc
      781 gaaggtcatg atgcgattga cggtgtcgta gcgcggcgcg atcgcgtcga acatgccgcg
      841 caccgcgcgt gccttctcct cgcccgccgg cagcggcttc actcgccggg ccatgcggcc
      901 tccaggcgcc ggcgcgactg ctcgcgcgtc gcggcgatct cggctgcgtc gccggtcttt
      961 tcgatgtgct cctcggcgac ctgcagcagc acgcggcgga agtggtgcgc gacgagcgcg
     1021 gtgacggccg gcagcagctc gccgaaggcg gcgacgagct tttcggccgc ctcctcgtcg
     1081 gacgaggcgg accgcttgat cggcgtgcgc acgtactcgt cgaacagccg cacggcctcc
     1141 tcggcgatgg cacgcgtcgc gtcgttctgc gctcgcgcga gcgcgatcag ctcgccgagc
     1201 gggagcccgg ccgacagaag ccgcaagccc gtgcggacca actcgacgtc ggaggcggcg
     1261 tagcgcgtct cgccgtcgat ctcgcggccg atcggaagcc cttcacgctc gatggatgcg
     1321 agcagcggcg ccggcacgcc cgaccgctcg gcgagctcct cgagcgagat gaactcctcg
     1381 gcgtcgccct cggcctgcgc ggaggcgacg gcggcggcca gatcctggtc ggcccggccc
     1441 agccggccgc tcacgacgcg ctcgatggct tgcagcgtca ggcccttctt ctgcaggctt
     1501 cgaatgcggc ggatccgctc ggcatgctcg gaggagtaca gcgcgacgcg cccgctgcgc
     1561 cggggcggcg tcagcaggcc tttggcctga tagaagcgga cggtgtcgac gcttacgtcg
     1621 cacgccgcgg ccagctgctc gacccgatac tccacgaccg ttaactctac gagtaaacac
     1681 tcgcagggtc cattcgctgg cggagcgcgc tacatcgcgg cgacgtcggc catggcggca
     1741 ccgatgaact cgtcgagacg ctcacggcct tcgctcggcc agccgatcgt cagatacgtg
     1801 aagccggcgt cggccttggc ctgcgcatcg gcacggacct cggccatcgt ctgcgacagg
     1861 ctcagcgcgg tcgaacggac gatcgaggac ggatcgcgtc cggccgtctc ggcgtggcgg
     1921 ctgagcatct cgctgcgctg cttgaggtcg tccgggcggc cgaacgtgtg ccacacgtcg
     1981 gcgtgacgcg ccgcgagcgc aaccatgcgc gggccgtgcc cgccgatcca gatcggcgga
     2041 tgcggctgct gaaccggctt ggggttgtag ccgccttcga ggcggaagtg acggccttcg
     2101 aaacgcgggg cgtcttcggt catcagcgcg cgcatgacct gcacggcttc ctcgagccgg
     2161 tctacccgct cgccggcggg cgggaactca agtccgagac gcctgtgctc gggttcgtgc
     2221 catgccgcgc cgaccgccag ctcgagccgg ccgttcgaga tgtgatcgac ggtgacggcc
     2281 tcggtcgcca gcagcgacgg atgccggtag gtgatgccgg tgacg
//
LOCUS       DATLFO010000132         7195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_104017, whole genome shotgun sequence.
ACCESSION   DATLFO010000132 DATLFO010000000
VERSION     DATLFO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104017"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VM841_04900"
     CDS             <1..568
                     /locus_tag="VM841_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVL89556.1"
                     /translation="MAEEHGATFLHPHNDPLIIAGHATAALEMFEDEPGIDTIVFPIG
                     GGTLIAGAALVAKTLKPEVRVIGVQAERLAAFALSRKEGHRLEVPPSTTFAEGVAVRE
                     PPVLPYGMVEDYVDDIVTVSEEEMRRGILLLLEKTHNLAEGAGAAGVAAVVQQRERLA
                     GRTVGTILSGGNLPWHVLNKALNDIHSW"
     gene            579..1772
                     /locus_tag="VM841_04905"
     CDS             579..1772
                     /locus_tag="VM841_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL89557.1"
                     /translation="MPKRQNPPLGFTTRAVHGLKSPGPVEQEPLSPPIYQAATFAFED
                     IDDFAAVAQSKISGGYLYSRWANPTVDALAGVVASLEGTEAAACFASGMAAIHGALTS
                     VLRAGDHVVASKHLYGGTFGLLNHILPRAGVEVSFIEPADLDEVQAAFTSRTKVLYVE
                     TIANPTMLVADLDALAVVARAGKATMIVDATFSSPYLIQAADHGADIIVHSATKYLGG
                     HSDVTAGVVAASAKAIAAIRTDAMEYGATLAPMEAWLTLRGIQTLSLRMERICGNALS
                     LAHYLANRNGVKAVHYPGLPSHPQHALAKQLMPRGFGGMLAFEITGGRKAGRAFLERV
                     RVAAPAASLGGTKTLVVHPASVTHTQLSAAQRRAGGITDGLIRVSVGIEDPEDLIADF
                     DRALG"
     gene            1816..2880
                     /locus_tag="VM841_04910"
     CDS             1816..2880
                     /locus_tag="VM841_04910"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HVL89558.1"
                     /translation="MSPKIRVAIVYGGRSAEHEVSVVSARCVIEAIDRDKYEVLPIAI
                     TKAGRWILPERGPEQLEAPKGSLPMVDEAGTDLALRPSGGLTAESGDGLGGVDVVFPL
                     VHGPFGEDGTLQGMLEMAGVPYVGAGVLASALGMDKEMQRRLFAARGLPLLPWIHVHE
                     SDRRRNEQGCVDLALDQIGLPAFTKPARLGSSVGVAKCKTKDDLREGLRLALRYDDKA
                     IVERAATARELECAVLGNDDAEASVVGEIVSAHEFYDYEAKYIEEASRTIVPAQVPSS
                     VSDQIRRLSIEAFAAVECRGMARVDFFYEEATGAVFLNEINTIPGFTPISMYPKLWEA
                     SGVPYAALIDRLIELALPER"
     gene            3042..3317
                     /locus_tag="VM841_04915"
     CDS             3042..3317
                     /locus_tag="VM841_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89559.1"
                     /translation="MNGRRLSRIIPALMALALVAAACTSNGESTDQGQQGTQGLLDRI
                     KNRGSVLCGVNNQVPGFGFVDQQGNFSGFDIEFCRVVAAAVLGTRRR"
     gene            3314..4129
                     /locus_tag="VM841_04920"
     CDS             3314..4129
                     /locus_tag="VM841_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVL89560.1"
                     /translation="MTFKPLTADQRFTALQSGEIDVLIRNTTWTAKRDGSEGAAFAAT
                     TFYDGQGMMVRADSKYQRLEDMGNTTICVLKGTTTETNLESQFRGRNITYTPLSFQDN
                     DTLREAFIQGRCQGWTSDKSQLAGIRSNFPAAQGGPEGLRILDVTLSKEPLGPVVRDG
                     DTKWFDAVNWATIATIAAEEFGLTKANVDQMKSSSTDPEVQRFLGVTPSGADPFDAGL
                     GLPADFAYQVVKQVGNYAEIYTAHVGPETPLGLPRGVNSLWTDGGLLYAPPYR"
     gene            4138..5319
                     /locus_tag="VM841_04925"
     CDS             4138..5319
                     /locus_tag="VM841_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVL89561.1"
                     /translation="MERERAADGAGLRPPPWRDVRVLRIAFQVLVVGIVAGLGLYLVG
                     NLTSNLDRQGIRTDFGYLSQPAGFAIADTSFRSTDSVLDALKVGIRNTAVLSLIGIVF
                     ALFLGIVVGVARLSRNWLVRKAAAVYVESLRNIPVLVIIIFFYIAVVLRLPPIQQASE
                     WFGAAVISNSGAVLPSLRDAGAANLFTLFVVAGFVAGAAVAWMRTRRFDRTGEPHHRV
                     LWFAGVFFVVAGAAYLVLGRPVAFSLPVRDGLVVAGGIRMPPEFAAMLVGLVVYTASH
                     IAEIVRGSILAVARGQSEAATALGLTDFQRLRFVVLPQAFRIMIPPLANQFLNLTKNS
                     SLAVFIGYADVASVTGIIVSQGNPAPQSIVLLMAVYLVFSLTISLVTNTVNRRMTLTT
                     R"
     gene            5316..6560
                     /locus_tag="VM841_04930"
     CDS             5316..6560
                     /locus_tag="VM841_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVL89562.1"
                     /translation="MTALPEETVRIPNEALRPPVHARSPREWIKENLFPNAFNSVLTV
                     VFGAIIMWAVVRTARFVFVTAQWSIVRANLTNFMVGFFPRDEIWRIWVAMMLLAVVTG
                     LRWGSGGRRRIAERTPQPLRQSLKPLWPPALLAVAIIVLSGSAVTAGLITALFLAGFG
                     GRIAGAALPQPIGRWTGVALAVTLVVAYVLVAGPGGSGASLWRGLLLTMALAAAGIAL
                     SFPIGVLLALGRRSSFPAIRVLCVGYIELIRGVPLITLLFMAFLILGFFLPPGAEIPT
                     LVTRALVALVLFTAAYLAEVVRGGLQAVPKGQIEAAQALGLSPVTITRRIVLPQALRA
                     VLPAIVGQFISLFKDTSLVGFIGLTELLGVAQQVTKQPEFLGLGLHAETLVFASFIYW
                     VGAYGMSRASQRMETRLGVGER"
     gene            6557..>7195
                     /locus_tag="VM841_04935"
     CDS             6557..>7195
                     /locus_tag="VM841_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVL89563.1"
                     /translation="MNEPMIVCDSVEKWFGDFRALKAVDVTVERTEVVVVMGPSGSGK
                     STLIRCINRLEKHDRGRIVVDGVEMSDDVRKIQEIRREVGMVFQQFNLFPHLTVMENI
                     TLAPRQVRHRPKAEAEQIAMEMLERVKIPEQARKFPGQLSGGQQQRVAIARALAMQPK
                     VMLFDEPTSALDPEMIKEVLDVMKELANTGMTMIVVTHEIGFAREVADRVLFF"
BASE COUNT         1139 a         2393 c         2419 g         1244 t
ORIGIN      
        1 catggcggaa gagcacggcg cgacgttcct tcatccgcac aacgacccgc tgatcatcgc
       61 cgggcacgcg acggccgcgc tcgagatgtt cgaagacgag ccgggcatcg atacgatcgt
      121 cttcccgatc ggcggcggga cgctcatcgc cggcgccgcg ctcgtcgcca agaccctcaa
      181 gcccgaggtg cgcgtcatcg gagtgcaggc cgagcggctc gcggccttcg ccttgtcgcg
      241 caaggaaggc catcgcctcg aggtgccacc ctccacgacg ttcgccgaag gcgtcgcggt
      301 tcgggagccg ccggtgctcc cgtacgggat ggtcgaagat tacgtggacg acatcgtgac
      361 cgtgtccgag gaggagatgc ggcgcggcat cctgctcctg ctcgaaaaga cgcacaacct
      421 cgcggaaggc gcgggcgcgg ccggcgtcgc cgccgtcgtt cagcagcgtg agcggctcgc
      481 cgggcggacc gtcgggacga tcctgtcggg cggaaacctg ccgtggcacg tgctcaacaa
      541 ggcgttgaac gacatccatt cctggtagga ggcctgcgtt gccgaaacga cagaacccgc
      601 cgctcggatt caccacgcgc gccgtgcacg gcttgaaaag ccccgggccg gtcgagcaag
      661 agccgttgtc gccgcccatc taccaggcgg ccacgttcgc cttcgaggac atcgacgatt
      721 tcgcggcggt cgcgcaatcg aagatctccg ggggctacct gtattcgcga tgggccaacc
      781 ccaccgtgga cgcgctcgcc ggcgtggtcg cctcgctcga gggcacggag gccgccgcat
      841 gcttcgcgag cggcatggcc gcgatccacg gcgccctcac gtccgttctt cgcgcgggcg
      901 atcacgtggt ggcgtcgaag catctctacg gtggaacctt cgggctgctc aaccacatcc
      961 tgccgcgagc gggtgtcgaa gtatccttca tcgagccagc ggacctcgac gaggtccaag
     1021 ccgcgttcac cagccggacc aaggtgctct atgtcgagac gatcgccaat cccactatgc
     1081 tcgtggccga tctcgacgcc ctggccgtgg tcgcgcgggc gggcaaggcg acgatgatcg
     1141 tcgacgcgac cttctcgtcg ccctatctga tccaggccgc cgaccacggg gccgacatca
     1201 tcgtgcattc ggccacgaag tacctcggcg gtcactccga cgtcaccgca ggcgtcgtgg
     1261 cggcctccgc gaaggcgatc gccgcgatcc gcaccgatgc gatggagtac ggcgcgacgc
     1321 tcgccccgat ggaggcgtgg ctgacgttgc gcggcatcca gaccctctcc cttcgaatgg
     1381 agcgcatctg cggcaacgcg ttgtcgctgg ctcattatct ggcgaatcgc aacggcgtga
     1441 aggcggtcca ctatcccggt ctgccgtcgc acccacagca tgccctggcc aagcagctca
     1501 tgccgcgcgg cttcggcgga atgctcgcgt tcgagatcac cggcggacgc aaggccggac
     1561 gggccttcct cgagcgtgta cgcgttgccg ctcccgccgc ctccctcggc ggcaccaaga
     1621 ccctcgtcgt gcatcccgcc tcggtcacgc acacgcaact gagcgcggca cagcgccggg
     1681 cgggcggaat caccgacggg ttgatccgcg tcagcgtcgg aatcgaggat cccgaagacc
     1741 tcatcgcgga cttcgatcgc gcgctcggat gagcgcgtcg cgcgcacgcg ctcccggcgg
     1801 tagtctgtcg cggtcatgag cccgaagatt cgcgtcgcga tcgtctacgg cggccgttcg
     1861 gccgagcacg aggtgtcggt cgtgtccgcg cgctgcgtca tcgaggcgat cgatcgcgac
     1921 aagtacgaag tgcttcccat cgcgatcacg aaggccggcc ggtggatcct ccccgagcgc
     1981 ggtccggaac agctcgaggc gcccaagggg tcgctgccga tggtggacga ggccggaacg
     2041 gatctcgcgc tgcgcccgag cggcgggctg acggccgagt ccggtgacgg gctcggtggt
     2101 gttgacgtgg tgttcccgct cgtgcacgga ccgttcggcg aggacggcac cctgcaaggc
     2161 atgctcgaga tggccggagt gccctacgtg ggcgccggag tcctcgcgag cgcgctcggg
     2221 atggacaagg agatgcaaag acggctgttc gccgcccgcg ggctgccgct gctgccgtgg
     2281 atccacgtcc atgaatccga ccggcgccgc aacgagcagg gctgcgtgga cctcgcgctc
     2341 gaccagatcg ggctgccggc gttcaccaag cccgcccgcc tcgggtccag cgtcggcgtg
     2401 gcgaaatgca agacgaagga cgatctgcgt gagggattgc ggctcgcgct tcgctacgac
     2461 gacaaagcga tcgtggaacg ggccgcgacc gcacgggagc tggaatgcgc ggtgctcggc
     2521 aacgacgatg ccgaagcgag cgtcgtcggt gagatcgtga gtgcgcacga gttctacgac
     2581 tacgaagcca agtacatcga agaggcgagc cgcacgatcg ttcctgcgca ggttccgtct
     2641 tcggtttcgg atcagatccg ccgcctgtcg atcgaggcgt tcgcggcggt cgaatgccgc
     2701 gggatggccc gcgtggactt cttctacgaa gaggcgaccg gcgcggtctt cctcaacgag
     2761 atcaacacga ttcccggctt cacacccatc tccatgtacc ccaaattgtg ggaagcatcg
     2821 ggtgttccgt acgcggcgct gatcgatcgt ctcatcgagc ttgccctgcc agaacgctga
     2881 gaatccgcag cggtgaggac gccgtatacc ggagtggtgc attccttgac acccctcgaa
     2941 gccaaactta ggctgacaga atcaggaaag cgcatagccg gatcagctac tccgtgacgg
     3001 cgcactccgt ttgggccgcc gccacgcgga aggggtgaag catgaacgga cggcgactgt
     3061 cccgaatcat tcccgcgctc atggcgctcg cgctcgtcgc ggccgcgtgc acaagcaacg
     3121 gagagagcac cgatcagggc cagcaaggca cgcaaggcct gctcgaccgg atcaagaacc
     3181 gcggcagtgt tctgtgtggc gtgaacaacc aggtgccggg attcggcttc gtcgaccagc
     3241 aaggcaactt ctcgggcttc gacatcgaat tctgccgcgt cgtggcggca gcggtgctcg
     3301 ggacgcgacg aaggtgacct tcaagcccct gaccgccgat cagcgcttca cggcactaca
     3361 gtcgggcgag atcgacgtgc tgattcgcaa cacgacgtgg acggccaagc gcgacggcag
     3421 cgaaggtgcg gcgttcgcgg cgacgacgtt ctacgacggg caaggcatga tggtccgcgc
     3481 cgacagcaag tatcaacggc tcgaggacat gggaaacacc accatctgcg ttctgaaggg
     3541 cacgacgacg gagaccaatc tcgagtcgca gttccgtggc cgcaatatca cctacacgcc
     3601 gctgtcgttc caggacaacg acacgcttcg tgaggccttc atccagggcc ggtgccaagg
     3661 atggacgtcc gacaagtcgc agctggcggg catccggtcg aacttccccg ctgcgcaagg
     3721 cgggccggaa ggactgcgga tcctcgacgt gacgctgtcg aaggagccgc tcggtcctgt
     3781 cgtccgcgac ggtgacacga agtggttcga cgcggtgaac tgggcgacca tcgcgaccat
     3841 tgccgccgaa gagttcggcc tgaccaaggc caacgtcgat cagatgaagt cgagctcgac
     3901 cgatcccgag gtgcagcgct tcctcggcgt gacgccgtcc ggcgccgatc cgttcgacgc
     3961 ggggctcggc ttgccggccg actttgccta tcaagtggtc aagcaggtcg gaaactacgc
     4021 ggagatctac actgcgcacg tcgggccgga aacgccgctc ggcctgcctc gcggcgtgaa
     4081 ctccctgtgg acggacggcg ggctcctgta cgccccgccg tatcgctaag gatccgattg
     4141 gagcgcgaaa gggcggcgga cggggccgga ctccggccgc cgccctggcg tgacgtccgg
     4201 gtcctgcgca tcgcgttcca ggtcctggtc gtgggcatcg tcgccgggct cggcctgtat
     4261 ctcgtcggca acctcacgtc gaacctcgat cgtcaaggca tccgcaccga cttcggatac
     4321 ctgtcccagc ccgccgggtt cgcgatcgcg gacacttcgt tccggtcgac cgactcggtg
     4381 ctcgacgcgc tgaaggtcgg catccgaaac accgccgtgc tctcgctgat cggcatcgtc
     4441 ttcgcgctgt ttctgggcat cgtcgtcggc gtcgcccgtc tgtcgcgcaa ctggctcgtg
     4501 cgtaaggccg ccgcggtgta cgtcgagtcg ctgcgcaaca tcccggtgct ggtcatcatc
     4561 atcttcttct atatcgcggt ggtgctgcgc cttccgccga tccagcaggc gtcggagtgg
     4621 ttcggcgcgg cggtgatctc caacagcggc gccgtgctcc cgtcgctccg cgatgccggc
     4681 gccgcgaatt tgttcaccct gttcgtggtg gccgggttcg tcgccggcgc ggccgtggca
     4741 tggatgcgga cgcgccggtt cgatcgcacg ggcgagcccc atcatcgcgt gctctggttc
     4801 gccggcgttt tcttcgtcgt ggccggggcc gcctacctgg tgctcggccg tccggttgcc
     4861 ttctcgctgc cggtccggga cgggctggtc gtcgcgggcg gtatccggat gccgcccgag
     4921 ttcgcggcga tgctcgtcgg cctggtcgtg tacaccgcca gccacatcgc cgagatcgtg
     4981 cgcgggagca tcctggccgt cgcgcgtggc cagagtgagg cggcgaccgc gctggggctg
     5041 accgactttc aacggcttcg cttcgtcgtg ctgccacaag cgtttcgcat catgatcccg
     5101 ccgctcgcga accagttttt gaacctgacc aagaattcgt cgctggcggt cttcatcggc
     5161 tacgcggacg tcgccagcgt caccggcatc atcgtcagcc aagggaaccc ggcgccgcag
     5221 tcgatcgtgc tgctgatggc cgtctatctg gtgttctcgc tcaccatctc gctcgtgacc
     5281 aacaccgtca accggcgcat gacgctcacg acccgatgac cgcgctgccc gaagagaccg
     5341 tgcgcatccc aaacgaggcg ttgcgaccgc cggtgcacgc ccgctcgcct cgggaatgga
     5401 tcaaagaaaa tctgttcccg aacgctttca acagcgtgct caccgtcgtg ttcggagcca
     5461 tcatcatgtg ggccgtggtg cgcacggcgc ggttcgtttt cgtcacggcg caatggagca
     5521 tcgtgcgcgc aaacctcacg aacttcatgg tcgggttctt cccccgcgat gagatctggc
     5581 gcatctgggt cgcgatgatg ctgctggccg tcgtgaccgg gctccggtgg gggagcggcg
     5641 ggcggcgccg catcgcggag cgcacaccgc agccactgcg ccagtcgctc aagccgcttt
     5701 ggccgcccgc gctgctggcc gtcgcgatca tcgtgctcag cggcagtgcc gtcaccgccg
     5761 gcttgatcac cgcgctgttt ctggcggggt tcggcgggcg gatcgccggg gcggcgctgc
     5821 cgcagccgat cggccgatgg acgggcgtcg cgctggccgt caccctcgtc gtggcgtacg
     5881 tgctggtcgc cggccccggc ggctcgggcg cctcactgtg gcgcgggctg ctgctgacga
     5941 tggcgcttgc ggcggccggc atcgccctgt cgtttccgat cggcgtgctg ctcgcgctcg
     6001 ggcggcgctc ctcgttcccc gcgatccgcg tgctctgcgt cggctacatc gagctgattc
     6061 gcggcgtgcc gctgatcacg ctgctgttca tggcgttcct gattctaggg ttcttcctcc
     6121 cgccgggagc cgagatcccg acgctggtca cccgggcgct cgtcgcgctc gtgctcttca
     6181 ccgcggccta cctcgccgag gtggtacgcg gcgggcttca ggccgttccg aaaggacaga
     6241 tcgaggccgc gcaggcgctg gggctttcgc cggtgacgat cacgcggcgc atcgtgcttc
     6301 cgcaggcgct gcgtgccgtg ctgccggcga tcgtcgggca gttcatcagc ttgttcaagg
     6361 acacgtccct ggtcgggttc atcggcttga ccgagctgct cggcgtcgcg cagcaggtca
     6421 ccaagcagcc ggagttcctc ggtctcggcc tgcacgccga gacgctggtg ttcgcgtctt
     6481 tcatctattg ggtcggcgcc tacggcatgt ctcgcgcgag ccagcggatg gaaaccagac
     6541 tcggagtcgg agagcgatga atgagcccat gatcgtatgc gacagcgtcg agaagtggtt
     6601 cggcgacttc cgggcgctca aggccgttga cgtgaccgtc gagcggaccg aggttgtcgt
     6661 ggtgatgggg ccgtccgggt cgggcaagtc caccctgatc cgctgcatca atcgcctcga
     6721 gaagcacgac cgcggccgca tcgtcgtgga cggcgtcgag atgagcgacg acgtgcgcaa
     6781 gatccaggag atccgccgcg aggtcggcat ggtcttccag cagttcaact tgttcccgca
     6841 tttgaccgtg atggagaaca tcacgctcgc gccccgtcag gtgcgtcacc ggccgaaggc
     6901 cgaggccgag cagatcgcca tggagatgct cgaacgcgtg aagatccccg agcaggcgcg
     6961 caagttcccc gggcagctat cgggaggtca gcagcagcgt gtcgcgatcg cgcgtgcgct
     7021 cgcgatgcag ccgaaggtga tgctcttcga cgagccgacc tccgcgctcg acccggagat
     7081 gatcaaggaa gtgctcgacg tgatgaagga gctggcgaac accggcatga cgatgatcgt
     7141 cgtgacgcac gagatcgggt ttgcgcgcga ggtggcagat cgcgtgctgt tcttc
//
LOCUS       DATLFO010000133         7416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_104097, whole genome shotgun sequence.
ACCESSION   DATLFO010000133 DATLFO010000000
VERSION     DATLFO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104097"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VM841_04940"
     CDS             complement(<1..282)
                     /locus_tag="VM841_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVL89564.1"
                     /translation="MPKGPGPAKAASGGRTVRFARRKEEGLAALVHQTIKVQEEERRR
                     VATEIHDGVTQQLVSIWFRVHACQRLLERGEIPAAVSELATMKQAIDETL"
     gene            436..831
                     /locus_tag="VM841_04945"
     CDS             436..831
                     /locus_tag="VM841_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89565.1"
                     /translation="MTISDLRCDFCGRLLAGSAWLEPGDAGAIRLFIHPGDPLLRDDQ
                     PLLCRVCWAALRSEIGEPDRADECAICGEPLTYTASLHLIEMTGRIGEAPAWQFCREH
                     GTALLNRFRFVEPKMAPADLALKADFPKR"
     gene            984..1226
                     /locus_tag="VM841_04950"
     CDS             984..1226
                     /locus_tag="VM841_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HVL89566.1"
                     /translation="MASRVQADKVLDVKGLLCPLPVIKLSKAIKEIDVGQIVEMQATD
                     PGAEPDMDAWVKNTGHEMVDHTAEGDVFTFWVRRTK"
     gene            1236..1613
                     /locus_tag="VM841_04955"
     CDS             1236..1613
                     /locus_tag="VM841_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HVL89567.1"
                     /translation="MDDDVKRILYVMTHGVESPERCATPFFLSAAAAAMENEVSIYFT
                     MNGPTLLRKGTAEKMGPKGDRGQPLSFFIDQALDVGVNLYVCQPSLDLHDMTMGDLID
                     GVKMIGGAAFNNMATESDAVISF"
     gene            1625..2362
                     /locus_tag="VM841_04960"
     CDS             1625..2362
                     /locus_tag="VM841_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HVL89568.1"
                     /translation="MALTAHPAHKIQKGRYDDVPHEEKARLFEEVKADPRYEDYLYGC
                     YECGICVAACPSARFYDFSPRRVAQATAREDVEQIYEQMNDDIWECSQCFSCLRCPRG
                     NNPGGIITIMREVAINNGMATAKEALEGYTRIIYKIMGTGTQVSPDMLQPDAFPDWGP
                     QVKEVSDQLELWRRMLPPETLHTTSTGWAIEPKTIMELYVIWHATGALDMIKDVDSNL
                     HMILEEMMEDRLEDADYDTSPVTSNGH"
     gene            2373..3560
                     /locus_tag="VM841_04965"
     CDS             2373..3560
                     /locus_tag="VM841_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HVL89569.1"
                     /translation="MPIPVEEILSKKAKFERDPERAPEDFHDELFKLEEEGEWIIQRV
                     PDDAIEVPTKYGRPKKVPRQPTWHHKSCGQCGHIPGYSTSIFWVIRKLGLDYIDPKDQ
                     TSCTAWNYYASATSNAAAQAAVAMRNFAAAYETGYFPIIHCGTSYGHYKEVREELLGH
                     ADLRKQVREVLGRLGKPMVMPEEIVHYSEWFHAMRHEIKAQQVVDMSNIAVTVHPACH
                     YHKLVEGDAIYDPDMYQGQRTSIVSGIAETLGAEVRNYSTWFDCCGFGFRHILVQRDF
                     TRSFATLRKIQVMKEEADPDVVLTHDTGCVTTLDKSQFAAKAHGVTASLPVMSEAQFA
                     ALAMGAHPYKVCQLHWHSADYKPLLEKMGIDWESAWAEFQEDMKKLERGEQEFLTWED
                     VDA"
     gene            3553..4611
                     /locus_tag="VM841_04970"
     CDS             3553..4611
                     /locus_tag="VM841_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL89570.1"
                     /translation="MPSDAVLIVGGGPGGLEAAATVVDLGGRVVLVEKHEKLGGTPIH
                     ENYAALTPHFESAEEGMQRMIDRVVGNPNADVRTQVTVASQEGSAGDFRVTLSDGSVV
                     EAGAVVIATGFQHFDPGRETQMYGYYEFPDVIALQDLEAMLKDHNVVRPSNGEKPKRL
                     CFIQCVGSRDRRIGNEYCSKVCCGIASKEAIEVRQQNPDCQVFIFYIDMRMYGYWENQ
                     IYWPAQEQHKVQYVKGIITEILPKDGRLLVRGEDTTMGRPMEVPMDIVVLSVGMEPSK
                     GTRDMAGIFEIPQNKYGFIETAAPPLDTVTTAKEGVFVCGAAIGPADLEDTISSAGAA
                     ASKAVALVRRTAAAGAPA"
     gene            4652..5365
                     /locus_tag="VM841_04975"
     CDS             4652..5365
                     /locus_tag="VM841_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89571.1"
                     /translation="MGGQVVDTLTAEEFMQQVLQKISVEEMDAIVTAAETKAARFREM
                     FSDGVPSRASLEAGLSMMFGIRRKSTGVIEEIGADRLAGLTADLLATGAVDERFDAFC
                     AGLPDHGDVAADIAGELLHFTAPEDYWLWRRWMWDPRTETGSLRLVTMDDFDLAGATP
                     GQTYVRVGQAMAFVTETGHAIGFAGRPPFGMDVFLACVYGIYMYTVLRMRMTQEFNHV
                     VPQLPELARRLLGVHRLEA"
     gene            5370..6641
                     /locus_tag="VM841_04980"
     CDS             5370..6641
                     /locus_tag="VM841_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVL89572.1"
                     /translation="MGLEKQYPAPIEKELAVIDGYELPGQWNRMFEQRVIWDYDAEQL
                     ERIGELPGGESLNWCYGCAKCVPVCPVDVVGEYGPRKIHRAVQAGQIDLFTDDNLWLC
                     TTCGNCLRVCPKEVDMIQIMPAVREVAVGEGNVPAELQQVFEKTARYGNALGENAKKR
                     TAWTKGANTEVRVLKDDPAPVDVLFLIEDYWAYHPRGQEAAQANARVLNRLGVDWAIL
                     GAEEKTVADSQRLAGEKGLFEMMMEENVAAYAKYEFKRIVTSDPHAFNALKNEYPKHG
                     HTYDVLHYTQLLATLADTMTFNKSLDMTVTFHDPCYLGRHNGEYDAPRTLIEKIPGIK
                     FVEMLRCKSNGYCCGGGGGGMWLDGLAAQHQTERLSERRVREAVETGAECLVVVCPYE
                     VSRFEDAVKSTGNDGKLVVRDLAELLDEAMA"
     gene            6641..>7416
                     /locus_tag="VM841_04985"
     CDS             6641..>7416
                     /locus_tag="VM841_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89573.1"
                     /translation="MLRLLDLGTVSGLRSQTWWHAVAETMQPGDAPVLALMTPGEPYV
                     SIGFHRRTGELDLAACAGRGLGIFRRRAGGGPVLCDDGQLFFQLIAPTSSIPAALDRA
                     WQRALGPAVDAFRSLGIDARLAPGNDMMCGDRKISGTGAAQIGEASVFVGNVIFDFDY
                     DEMADILALPPHAKSEAARLMRRYLGPVSDVAGRPVTREEAAGALIASYGDAFGGMIP
                     SSPTAIEAQAAARLDDRFSDPAWVFAERAPAPPRVKIRGGV"
BASE COUNT         1344 a         2384 c         2467 g         1221 t
ORIGIN      
        1 cagcgtctcg tcgatggcct gcttcatcgt ggcgagctcg gaaacggcag cgggaatctc
       61 gccgcgctcc aaaaggcgct ggcaggcgtg cacccggaac cagatggaga cgagctgttg
      121 ggtaacgccg tcgtggatct cggtggcgac gcgccggcgc tcctcctcct gaaccttgat
      181 ggtctggtgg acgagggccg cgagcccttc ttccttgcgc cgggcgaagc ggaccgtgcg
      241 ccctccggac gctgccttcg ccggtcctgg tcccttcggc atgcccctcc ccgacattcg
      301 aacattcgca tttgcgattg ttgcactccg ttcagaccgg gacaaggggt ccggggatgt
      361 ggccggggga tcgtcgatcc ctcgatccag tgatcttgcc cgctcggccg tcctcgcccg
      421 ctaggcttgc gcgccatgac catctcggat cttcgctgcg acttctgtgg ccgtctcttg
      481 gcgggatcgg cctggctcga gccgggggat gccggggcca tcaggctgtt catccatccg
      541 ggggacccct tgctccggga cgatcagccg ctgctgtgcc gggtgtgctg ggccgcgctc
      601 cgctcggaga tcggcgagcc ggatcgcgcc gacgaatgcg cgatctgcgg agagccgctg
      661 acctatacgg cgagccttca tctgatcgag atgaccggcc ggatcggtga ggctccggcg
      721 tggcagttct gtcgcgagca cgggaccgct cttctgaacc ggttccggtt cgtcgagccc
      781 aagatggcgc ccgccgatct cgcgctcaag gcggacttcc caaagcgtta ggcggccggc
      841 cgggggctgt gctttcgtcc gatttcctga gtcagaatca ggacaaatca tcacgagaaa
      901 cccttgaccg caacattcga atatggtcat atggctttgc gttcctgcac gaaacagggc
      961 gcggaacggg aggggtgagg cctgtggcaa gtcgcgtcca ggcggacaag gtcctcgatg
     1021 tgaaggggct cctttgtcct ttgccggtga tcaagctctc caaggcgatc aaggagatcg
     1081 acgtcggcca gatcgtcgag atgcaggcga ccgatccggg cgcggagccc gacatggacg
     1141 cctgggtgaa gaacaccggg cacgagatgg tcgatcacac cgcggagggt gacgtgttca
     1201 ccttctgggt gcgtcgaacg aagtaggggg gcgttgtgga cgacgacgtc aagcgcatcc
     1261 tctacgtgat gacccacggc gtcgagtcgc cggagcgctg cgcgacgccg ttcttcctgt
     1321 ctgccgccgc ggccgccatg gagaacgagg tgtccatcta cttcaccatg aacggcccga
     1381 cccttctgcg gaagggcacc gccgagaaga tggggccgaa aggcgaccgc gggcagccgc
     1441 tgtctttttt catcgatcag gcgctcgacg tcggtgtgaa tctctacgta tgtcagccga
     1501 gcctcgacct tcacgacatg accatgggcg acctcatcga cggcgtgaag atgatcggcg
     1561 gcgccgcctt caacaacatg gcaaccgaat ccgacgccgt gatctcgttc taacggagga
     1621 ctccgtggcc ctgaccgctc atccagcgca caagattcaa aaaggccgct acgacgacgt
     1681 tccccacgag gagaaggcgc gtctgttcga ggaagtgaag gccgaccccc gctacgagga
     1741 ttacctctac ggctgctacg aatgcgggat ctgcgtcgcg gcctgtccgt cggcccgctt
     1801 ttacgacttc tcgccccgcc gcgtcgcgca ggcgaccgcg cgcgaggacg tcgagcagat
     1861 ctacgagcag atgaacgacg acatctggga gtgctcccag tgcttctcgt gtctgcgttg
     1921 cccgcgaggc aacaacccgg gcggcatcat caccatcatg cgcgaggtcg cgatcaacaa
     1981 cgggatggcg accgcgaagg aagcgctcga aggctacacg cgcatcatct acaagattat
     2041 gggcaccggc acgcaggttt cgccggacat gctccagccc gacgcattcc ccgactgggg
     2101 cccgcaggtc aaggaggtct ccgatcagct cgaactgtgg cgccgcatgc ttccgccgga
     2161 gacgctgcac accacatcca ccggctgggc gatcgagccg aagacgatca tggagctcta
     2221 cgtcatctgg cacgccaccg gcgcgctgga catgatcaag gacgtcgact cgaacctgca
     2281 catgatcctc gaagaaatga tggaagaccg gctcgaggac gcggactacg acacttcgcc
     2341 cgtcacgagc aacggtcact gaggaggccg gcatgccgat tcctgtcgaa gagattctga
     2401 gcaagaaggc gaagttcgag cgggaccccg agcgcgcgcc cgaagacttc cacgacgagc
     2461 tgttcaagct cgaggaagag ggcgagtgga tcatccagcg cgttccggac gacgctatcg
     2521 aggttcccac caagtacggc cggcccaaga aggttccgcg tcagccgacc tggcatcaca
     2581 agtcctgcgg tcagtgcggt cacatccccg gctactcgac gtcgatcttc tgggtgatcc
     2641 gcaagctcgg gctcgactac atcgacccga aggaccagac gtcgtgcacc gcgtggaact
     2701 actacgcctc cgcgacctcg aacgccgccg cgcaggcggc cgtcgcgatg cgcaacttcg
     2761 ccgccgcata cgaaacgggc tacttcccga tcatccactg cggcacctcc tacggtcact
     2821 acaaggaagt ccgtgaggag ctgctcggcc acgccgatct tcgcaagcag gtgcgcgagg
     2881 ttctcggccg gctcggcaag ccgatggtca tgcccgagga gatcgtgcac tacagcgagt
     2941 ggttccacgc catgcgccac gagatcaagg cccagcaggt ggtggacatg agcaacatcg
     3001 cggtgacggt ccacccggca tgccactacc acaagctcgt cgaaggcgac gcgatctacg
     3061 accccgacat gtaccaggga cagaggacct ccatcgtttc cggaatcgcc gagacgctcg
     3121 gggccgaggt ccgcaactac tcgacctggt tcgactgctg cggcttcggc ttccgccaca
     3181 tcctggtgca gcgggacttc acacgctcgt tcgccacgct gcgcaagatc caggtcatga
     3241 aggaagaggc cgacccggac gtggtgctca cccacgacac cggatgcgtc accaccctgg
     3301 acaagagcca gttcgccgcg aaggcgcacg gcgtgactgc gtcgctcccg gtgatgagcg
     3361 aagcgcagtt cgccgcactc gcgatgggcg cgcatccgta caaggtctgc cagctccact
     3421 ggcactcggc cgactacaag ccgctgctcg aaaagatggg gatcgactgg gagtccgcgt
     3481 gggccgagtt ccaggaggac atgaagaagc tcgagcgagg cgaacaagaa ttcctcacct
     3541 gggaggacgt tgatgcctag cgatgccgtt ctgatcgtcg gtggaggccc gggcgggctc
     3601 gaagccgcag cgaccgtggt ggacctgggc ggtcgtgtgg ttctggtcga gaagcacgaa
     3661 aagctcggcg gtacgccgat ccacgagaac tatgccgccc tgacgccgca tttcgaatcc
     3721 gccgaagagg gcatgcagcg tatgatcgac cgcgtcgtcg gcaaccccaa cgcggacgtg
     3781 cgcacgcagg tcaccgtcgc ctcgcaggag ggctcggcgg gcgacttccg ggtcacgctt
     3841 tcggacgggt cggtcgtcga agccggcgcg gtcgtcatcg caacgggctt ccagcacttc
     3901 gatccgggcc gcgagaccca gatgtacggc tactacgagt tccccgatgt gatcgcgctg
     3961 caggatctcg aggcgatgct caaggatcac aacgtcgtgc gtccctcgaa cggggagaag
     4021 cccaagcgtc tgtgcttcat ccagtgcgtg ggatcccgcg accggcgaat cgggaacgag
     4081 tattgctcga aggtgtgctg cggaatcgcg tccaaggaag cgatcgaggt ccgtcagcag
     4141 aatccggact gccaggtctt catcttctac atcgacatgc gcatgtacgg ctactgggag
     4201 aaccagatct actggccggc gcaggagcag cacaaggtcc agtacgtcaa gggaatcatc
     4261 accgagatct tgcccaagga cggccgtctg ctggtccgcg gcgaggacac gacgatgggt
     4321 cgcccgatgg aggtccccat ggacatcgtc gtactgtcgg tcggcatgga gccctcgaag
     4381 ggcacgcgcg acatggccgg catcttcgag atcccgcaga acaagtacgg gttcatcgag
     4441 accgccgctc cgccgctcga cacggtgacg accgcgaagg aaggcgtctt cgtgtgcgga
     4501 gcggcgatcg gtccggccga cctggaggac acgatctcga gcgcaggtgc ggccgcctcg
     4561 aaggccgtcg cgctcgtgcg gcgcacggcc gcggccggcg cgccggcgta gggtcgccgc
     4621 aaaaccaacg aggggacggg ggttcgacag cgtgggcgga caggtcgtag acaccctcac
     4681 cgccgaggag ttcatgcagc aagtgctgca gaagatctcg gtggaagaga tggatgcgat
     4741 cgtgacggcc gccgaaacca aggccgcgcg cttccgcgag atgttctcgg acggcgtccc
     4801 ttcgcgtgcc tcgctcgaag ccggcctgtc gatgatgttc ggcatccggc gcaagagcac
     4861 cggcgtgatc gaggagatcg gcgccgaccg gcttgcaggg ctgaccgctg atctcctcgc
     4921 caccggcgcg gtcgacgagc gtttcgacgc gttctgcgcc gggctgcccg accacggcga
     4981 cgtcgcggcc gacatcgcgg gcgagctgct gcacttcacg gccccggagg actactggct
     5041 ctggcgccgc tggatgtggg acccgcggac cgaaaccggc tcgttgcgtc tggtcaccat
     5101 ggacgatttc gatctcgccg gggcgacgcc cggccagacc tacgttcgtg tcggacaggc
     5161 aatggcgttc gtgaccgaga ccggtcatgc gatcggattc gcgggacgtc cgccgttcgg
     5221 catggacgtg ttcctggcct gcgtctacgg catctacatg tacaccgtgc ttcgtatgcg
     5281 gatgacgcaa gagttcaacc acgtcgtccc gcagctgccc gagctcgcgc gccgtctgct
     5341 gggcgtgcat cgactggagg cgtagcccga tgggtttgga gaagcaatat ccggcaccga
     5401 tcgagaagga actcgccgtc atcgacggct acgagctgcc gggtcaatgg aaccgcatgt
     5461 tcgagcagcg cgtcatctgg gactacgacg ccgagcagct cgagcggatc ggcgagcttc
     5521 ccgggggcga gtcgttgaac tggtgctacg gctgcgcgaa atgcgttccg gtctgccccg
     5581 tcgacgtggt gggcgaatac gggccgcgca agatccaccg cgccgtccag gccggtcaga
     5641 tcgacctgtt caccgacgac aacctctggt tgtgcacaac ctgcggcaac tgtctgcgcg
     5701 tgtgcccgaa ggaggtcgac atgatccaga tcatgccggc cgttcgcgag gtcgccgtcg
     5761 gcgagggcaa cgtgcccgcc gagcttcagc aggtcttcga gaagaccgcg cgctacggaa
     5821 acgctctcgg tgagaacgcc aagaagcgca ccgcgtggac caagggcgcg aatacggagg
     5881 tgcgggtcct gaaggacgat cccgcgccgg tggacgtgct gttcctgatc gaggactact
     5941 gggcctacca cccccgtggc caggaggcgg cgcaggcgaa cgcccgcgtg ctcaaccggc
     6001 tcggggtgga ctgggcgatc ctgggcgcgg aagagaagac cgtcgccgac tcgcagcgtc
     6061 tcgcgggcga gaagggtctg ttcgagatga tgatggaaga aaacgtcgcg gcctacgcca
     6121 agtacgagtt caagcgcatc gtcacctctg atccgcacgc gttcaacgcg ctcaagaacg
     6181 agtatcccaa gcacggccac acctacgacg tgctgcacta cacgcaactg ctcgcgacgc
     6241 tggccgacac gatgaccttc aacaagtcgc tggacatgac ggtcaccttc cacgacccgt
     6301 gctacctcgg ccggcacaac ggcgagtacg acgcgcctcg gaccctgatc gagaagatcc
     6361 ccggcatcaa gttcgtcgag atgctgcggt gcaagtccaa cggctactgc tgcggcggcg
     6421 gcggcggcgg catgtggctg gacgggctgg ccgcacagca ccagaccgag cggctatccg
     6481 agcggcgggt gcgtgaagcg gtcgagaccg gcgcggagtg tctcgtcgtc gtctgcccct
     6541 acgaggtttc gcgcttcgag gacgcggtca aatccaccgg caacgacgga aagctggttg
     6601 tgcgcgacct ggccgaactg ctcgacgagg cgatggcttg atgctgaggc tgctcgatct
     6661 cggaaccgtg tcgggtctgc gctcgcagac ctggtggcac gcggtcgccg agacgatgca
     6721 gcccggggac gcgccggtgc tcgcgctgat gacgcccgga gagccctacg tctcgatcgg
     6781 atttcaccgc cggaccggcg agctcgatct cgcggcgtgc gcgggccggg gccttggcat
     6841 ctttcgccgg cgagcgggtg gcggaccggt cctgtgcgac gacggccagc tgttcttcca
     6901 gctgatcgcg ccgacgtcgt cgatcccggc cgcgctggac cgcgcgtggc agcgggcgct
     6961 cggcccggca gtcgacgcct ttcggtcgct cggaatcgac gcgaggctcg cgcccggcaa
     7021 cgacatgatg tgtggggacc gcaagatcag cggaaccggc gctgcccaga tcggtgaggc
     7081 tagcgtgttc gtcggcaacg tcatcttcga cttcgactac gacgagatgg ccgacatcct
     7141 ggcgctgccc ccgcacgcga agagcgaagc ggcacggctg atgcggcgct accttggacc
     7201 ggtgagcgac gttgccggcc gcccggtgac gcgagaggag gccgcaggag cgctcatcgc
     7261 gtcctacggc gacgccttcg gcgggatgat tccgtcctcg ccgaccgcaa tcgaggctca
     7321 ggccgcggcc cggctcgacg atcgcttctc cgatccggcg tgggtgttcg ccgagcgggc
     7381 ccccgcgccg ccgcgcgtga agatccgtgg gggcgt
//
LOCUS       DATLFO010000134         9389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_93102, whole genome shotgun sequence.
ACCESSION   DATLFO010000134 DATLFO010000000
VERSION     DATLFO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93102"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VM841_04990"
     CDS             complement(<1..1526)
                     /locus_tag="VM841_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL89574.1"
                     /translation="MSTPKKRSKDWVGPIPYGIGEVKPNHYLEMARIAWENRSHPWYA
                     WKILRDGVCDGCALGTTGLHDWTMDGVHLCTVRLNLLKLNTMDAMDHRVLADVRNVEA
                     LSSAQMRDLGRLGSPMLRRRGEAGFRRVSWQQALGLAGDALRETDPRRMGFYLTSRGI
                     TNEVYYVAQKVARFLGTNNVDNSARLCHAPSTVAMKQMLGVSASTCSYKDWIGSDLIV
                     FVGSDVANNQPVTTKYLYYAKKQGTRIAMVNPYAEPGMRRYWVPSVAESAMFGTKITD
                     EFYPVHTGGDLAFFNGVLKHLIEQAWVDLPWIAAHTKGFDVAADAVAAQPWEMLEAAS
                     GASRDQMLAFARMLSQARSGIFVWSMGATQHRHGVDTVRAILNVALALGWVGREHCGV
                     MPIRGHSGVQGGAEVGCVPNTFPGGRAVGEDTAREMSALWGFDVPSVPGLATADQIDA
                     AHRGDLDLLWSAGGNFLETMPEPAYVREALERIPFRIHQDIVLTSQMLVEPREWVLIL
                     P"
     gene            1586..3754
                     /locus_tag="VM841_04995"
     CDS             1586..3754
                     /locus_tag="VM841_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HVL89575.1"
                     /translation="METFFRTCPLCEATCGLAITMDGDRVAGIRGDDEDVFSHGYICP
                     KGTTLGDLHHDPDRIRRPLIKRDGRFVEATFDEAFAEIERRLPPILAEHGRDAVGLYI
                     GNPTVHNMDSTIFVKPMAKAIGTRNIFSASTVDQRPKELACAWMYGGGLTVPIPDIDR
                     TDYLLILGANPLDSNGSLATAPDWPGRLKAIRKRGGRVVVVDPRRTNTAAKADEHIAI
                     RPGGDAFFLAAVAHTIFDEGLVTLGTVDGHVSGLDEAREAVEPFTPDAVAAACGIDAA
                     TIVRIARELAAAPSAAVYGRIGTTTQEFGTLASWLVDVCNIVTGNMDRPGGVMFPKAA
                     AGASNTRPGKGRTFRIGRFTSRVRGAAETLGELPSACLAEEIDTAGEGQIRAMITIAG
                     NPVLSTPDGARLDRALAGLDFMVSVDVYLNETTRHADVILPPPEPLQRSHYDLAFWQL
                     SVRNVANYSPAILPLQDGQMHEWEILSVLATIAQTGTRADPSIVDDLVAMTVAQGVAT
                     ESLTAEQLLEATAARRGPERILDIMLRGGPYDLTLDDLLAAPHGVDLGPLQPRIPEIL
                     RTESGKIECAPPPLIADLARLRAALDRPANGMMLIGRRHLRSNNSWGHNVPAMVKGRD
                     RCTLMIHPADATRLGLEDGKPATIVSGTGTLVAPVEITDDIREGVVSLPHGWGHDLPG
                     IRLSVASSKPGVNTNLLTPSVMDALSGNAVLNGIPVEISA"
     gene            complement(3904..4059)
                     /locus_tag="VM841_05000"
     CDS             complement(3904..4059)
                     /locus_tag="VM841_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89576.1"
                     /translation="MGTKFVVGLANTGIGLGAAIWTAVAGAASLAAGAVTLGRGRRVS
                     TGESLGE"
     gene            complement(4119..5711)
                     /locus_tag="VM841_05005"
     CDS             complement(4119..5711)
                     /locus_tag="VM841_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL89577.1"
                     /translation="MTRDRDVVEPDSAGAVGVFVVGGAGGEALRETVVATGALHVWCA
                     VPSITEAAAVTGLADSFVLDPWPGPAAALDYLARSTQGKRSGMAIILLRAGTILRRGT
                     VRRATSRLRDHEGLVVPTRRGDRGTAIRLRMFRRAPLDPGSGVIVSAVLLFWLVRAGW
                     LTGTPLTIGLLRRAGARLHSLRRGLASPSKAPRQDRPTITVLIPARNESAYLGETLRS
                     LEAQSIVPDEVIVVDDASDDGTGDIARAHGARVIRPGSAQGSKAIASNAGLADVRTEA
                     VMILDADTQLHPECLEHLSDDLRRGLDATSGACLPLLQQGIWARGRAIEYSMAIRIFK
                     PVQRALGTLVVMSGCNSMFRTSLVRGLGGFSDRTLAEDLDLTWAHQLQGGKAGYNGRA
                     MSYPVEPATWTLYRAQIRRWASGFYQTIGVHRMRLRHKPGLALIVAAAVFDVITVPIF
                     LTMLTIATIKGSFSWPFLAMSSAFLLLPIISGITVIGWRKTAKNFPAYLITIWASSYF
                     YLEALIIEWIARRHRVAWVKGH"
     gene            complement(5708..6229)
                     /locus_tag="VM841_05010"
     CDS             complement(5708..6229)
                     /locus_tag="VM841_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89578.1"
                     /translation="MNDPSGMTSIRMHSSEIAAREFPTARQGLDPAAVRAWLTMVSAF
                     VADLEDELAHARNEREQTSASIRRMRRRPPSAEIRGALMQAQLRRRVGGYDRHEVQEL
                     LRAAASELARLENEVGLLNTELELQLEVEREPLPEPLPSVLRRAEKTVIRRGNVASLH
                     QDSDPPALHKSVL"
     gene            complement(6372..7352)
                     /gene="moaA"
                     /locus_tag="VM841_05015"
     CDS             complement(6372..7352)
                     /gene="moaA"
                     /locus_tag="VM841_05015"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HVL89579.1"
                     /translation="MSLVDGFGRVADDLRISVTDKCNFRCTYCMPAEGLKWLPRDEIL
                     TFEEIDRLAGIFVGLGVKTIRLTGGEPLMRRDLSRLVGMLNDRGVPDLSLTTNGFWLA
                     GQAKELAEAGLKRINVSVDSLLRHRFAEMTRRDALEAVMKGLKAAEEAGLAPIKLNAV
                     VVRGTNDDEIVDFARFARDTGYQVRFIEFMPLDADEAWERSAVVPSAEVLDRIRDAFA
                     LEPVEHGPEPATVYRFADGAPGGVGVISSVTEPFCDSCNRVRITAEGRLRNCLFALGE
                     TDLRAMLRGEASDDQIIEAITANVAGKWRGHLIDRPGFKRPGRSMSMIGG"
     gene            complement(7349..8269)
                     /locus_tag="VM841_05020"
     CDS             complement(7349..8269)
                     /locus_tag="VM841_05020"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HVL89580.1"
                     /translation="MTNERWDVTLARRASRRHGVVSRAEALAAGASASAIQRRLALGR
                     WRLIFPGVYVVEGNPPSWRQTLLAACLAAGPDAVASHTAAAALRSLAGFKESRIEITA
                     ARSGVRHRRFTVHRVRSLPASEVTRVDGIPVTTVARTLVDLAATQPLDSVEIALDDAL
                     HRRLVTAAQIRRVIERDRIRGGPAIRDLLAARPAGVTVPESPLETMFVRLVHDHGLPQ
                     PTHQHALRLGGRRMRLDFAYPQAKVAIEADGYRHHNGRRSWESDRERRNLLTAAGWRV
                     LQITWTDVDEHPAETASRIRRLLATPILGE"
     gene            complement(8406..9161)
                     /locus_tag="VM841_05025"
     CDS             complement(8406..9161)
                     /locus_tag="VM841_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89581.1"
                     /translation="MDSALHVTQVRSGPAVRSQQVLPWSYRFFGSALVGRAPQQLPLG
                     VRATLLWALAEAARYVHAGRARPDEEEVLGLRLAYQALKAEAATVRAAADSPGEMLGA
                     AIALRHRQRAAVLMRLGLKMSESDVARVLGVRPAQVQPIIAGARAAVARRLGRPVNLA
                     RVMRAAATPVPEAAPPAEGSVAKVVRLPRPSPVQVLIAPDLPAATEPQPAPTVSGVPA
                     NIGRRRSVWSLIGRVAAAATVGMLFAWAIWPVH"
BASE COUNT         1517 a         3317 c         3126 g         1429 t
ORIGIN      
        1 ggaaggatca gaacccactc gcgcggctcg accagcatct gggacgtgag cacgatgtcc
       61 tgatggatgc ggaacgggat gcgctcgagc gcctcacgca cgtaggccgg ctcgggcatg
      121 gtttcgagga agtttccgcc cgcggaccac agcaggtcca gatcgccgcg gtgcgcggcg
      181 tcgatttggt cggcggtggc aaggcccggc acggacggaa cgtcgaagcc ccacagcgcg
      241 ctcatctcgc gtgcggtgtc ctcgccgacg gcacgcccgc ccggaaaggt attgggcacg
      301 cagccgacct cggcgccgcc ttgcacgccg ctgtggccgc ggatcggcat gaccccgcaa
      361 tgctcgcggc cgacccagcc gagcgcgagc gccacgttga ggatcgcgcg cacggtatcg
      421 acgccgtggc gatgctgggt cgcgcccatc gaccaaacga agatcccgct ccgcgcctgc
      481 gacagcatgc gtgcgaacgc cagcatctga tcgcgcgacg cacccgaggc cgcctcgagc
      541 atctcccacg gctgcgcggc gacggcgtcg gcggcgacgt cgaagccctt cgtgtgcgcg
      601 gcgatccacg gcaggtccac ccacgcctgc tcgatcagat gcttgagaac gccgttgaag
      661 aacgcgagat ccccgccggt gtggaccggg tagaactcgt cggtgatctt ggttccgaac
      721 atcgccgact ccgcgaccga cggcacccag taccgccgca tgcccggctc ggcatacggg
      781 ttgaccatcg cgatgcgcgt gccttgcttc ttcgcgtagt agaggtactt ggtcgtgacg
      841 ggttggttgt tggcgacgtc gctgccgacg aacacgatca ggtcgctgcc gatccaatct
      901 ttgtaggagc aggtcgaggc gcttacgccc agcatctgct tcatcgcgac cgtggacggc
      961 gcgtgacaca gccgcgccga gttgtccacg ttgttggtgc cgaggaaccg cgcgaccttc
     1021 tgcgcgacgt agtagacctc gttggtgatt ccgcgcgagg tcaggtagaa gcccatccgc
     1081 cgcggatcgg tctcgcgcag cgcgtcgccg gccagtccca gcgcctgctg ccacgacacc
     1141 cgccggaagc ccgcctcgcc gcgccgccgc agcatcgggg atccgagccg gccgaggtcg
     1201 cgcatctggg cgctcgacag cgcctcgacg ttgcgcacgt cggccaggac gcggtgatcc
     1261 atcgcgtcca tcgtgttgag cttgagcagg ttgagccgca ccgtgcacag atgcacgccg
     1321 tccatggtcc agtcgtgcaa gccggtcgtg ccgagcgcgc agccgtcgca gacgccgtcc
     1381 cgcaggatct tccacgcgta ccagggatgg ctccggttct cccacgcgat gcgggccatc
     1441 tcgaggtagt ggttgggctt gacctcgccg atgccgtatg ggatcggccc gacccaatcc
     1501 ttcgaccgct tcttcggcgt gctcatcgac gcgagtatag ggacgtcgca ttgacttcgc
     1561 gggagcaagc cgggatagtg cccgtatgga gaccttcttt cgcacctgtc ccctgtgcga
     1621 ggccacctgc ggcctggcga tcacgatgga cggcgaccgc gtcgccggca tccgcggcga
     1681 cgacgaggat gtgttcagcc acggctacat ctgcccgaag ggcacgacgc tgggcgacct
     1741 gcaccacgac cccgaccgga tccgccgtcc gctgatcaaa cgcgacggcc gcttcgtcga
     1801 ggccaccttc gacgaggcgt tcgccgagat cgagcgccgc ctgcccccga tcctggccga
     1861 gcacggccgc gatgccgtcg gcctgtatat agggaacccc acggtccaca acatggactc
     1921 gacgatcttc gtgaagccga tggccaaggc gatcggcacg cgcaacatct tttcggcctc
     1981 gacggtggac cagcgcccga aggagctggc ctgcgcgtgg atgtacggcg gcgggctgac
     2041 cgtcccgatc cccgacatcg accggaccga ctacctgctg atcctcggcg cgaacccgct
     2101 cgactcgaac ggctcgctcg cgaccgcacc ggactggccg ggccggctca aggcgatccg
     2161 caagcgcggc ggcagggtcg tggtcgtgga tccgcgccgc acgaacaccg ccgccaaggc
     2221 cgacgagcac atcgcgatcc gtcccggcgg cgacgcattc ttcctcgccg cggtggcgca
     2281 cacgatcttc gacgaggggc tggtgacgct cggcaccgtc gacggtcacg tcagcggcct
     2341 cgacgaggcc cgcgaggccg tggagccgtt cacgcccgac gcggtcgcgg ccgcgtgcgg
     2401 catcgacgcg gccaccatcg tgcgcatcgc gcgcgagctc gcggccgcac cgtccgccgc
     2461 ggtctacggc cgcatcggca cgaccacgca agagttcggc acgctggcgt cctggctggt
     2521 ggacgtgtgc aacatcgtga cgggcaacat ggaccggccc ggcggcgtga tgttccccaa
     2581 ggccgccgcg ggcgcgtcca acacccgtcc cggcaaggga cgcacgttcc ggatcggccg
     2641 cttcacctcg cgcgtgcgcg gggcggccga gacgctcggc gagctcccgt ccgcctgcct
     2701 ggccgaagag atcgacaccg cgggcgaggg acagattcgc gcgatgatca ccatcgcggg
     2761 aaaccccgtg ctgtccacgc ccgacggtgc gcgcctggac cgcgcgctgg ccggtctcga
     2821 cttcatggtc agcgtggacg tctatctgaa cgagaccacg cggcacgccg acgtgatcct
     2881 gccgccgccc gagccgctcc agcgctcgca ctacgacctg gcgttctggc agctgtcggt
     2941 tcgcaacgtg gcgaactatt caccggcgat ccttccgctg caggacggac agatgcacga
     3001 gtgggagatc ctgtcggtgc tcgcgacgat cgcgcagacg ggcacacggg cggacccctc
     3061 gatcgtggac gatctggtcg cgatgaccgt cgcgcaggga gtcgcgacgg agtcgctcac
     3121 cgccgagcag ctgctcgagg cgaccgcggc ccgccgcggc cccgagcgga tcctcgacat
     3181 catgcttcgc ggcggcccgt acgacttgac gctcgacgac ctgctcgccg cgccgcacgg
     3241 cgtcgacctc ggcccgcttc agccgcgcat ccccgagatc ctgcgcaccg aatccggaaa
     3301 gatcgagtgc gcgccgccgc cgctgatcgc ggacttggcc cggctccgcg ccgcgctgga
     3361 ccggcccgcg aacggcatga tgctgatcgg gcggcggcat ctgcgctcga acaactcgtg
     3421 gggccacaac gtgcccgcga tggtcaaggg ccgcgaccgc tgcaccctga tgatccatcc
     3481 ggccgacgcc acccggctcg gcctggaaga cggaaagccc gcaacgatcg tcagcggcac
     3541 cggcacgctg gtcgcgccgg tcgagatcac cgacgacatc cgcgaaggcg tcgtgtcgct
     3601 cccccacggg tggggccacg acctgccggg gatccggctg tcggtcgcgt cgtccaagcc
     3661 gggcgtgaac accaacctgc tgaccccgtc ggtcatggac gcgctgagcg gcaacgcagt
     3721 cctgaacgga atccccgtcg agatatcggc ctagccgccg cgctctcccg gtctccaccg
     3781 ggggggacac gtaccgcatg gtcggctcac cgagtgagcc ggttgacgcc cccgggcgcg
     3841 cccgaggcgc cggcgcctct tttcggcacc ggcacactcg gcggcttgcg ggcgggaacg
     3901 gctctactcg ccgagtgact ctccggtcga aacgcgacgg cctctcccaa gcgttaccgc
     3961 gcctgccgcg aggctcgccg cgccggcgac ggcggtccaa atcgccgcgc cgaggccgat
     4021 cccggtgttc gccagcccga cgacaaactt ggttcccaag acagcggtgg ttccgccacc
     4081 tgctgccggc acagaaatag acatgctctg ctcctttttt agtgtccctt gacccaggcg
     4141 acacggtgcc gcctggcgat ccattcgatg atcaatgctt cgagatagaa gtacgagctc
     4201 gcccaaatag tgatgagata tgcaggaaag ttcttcgcgg tctttcgcca cccgatgacg
     4261 gtgatgccgg agatgatcgg gaggagcagg aacgcggacg acatagcaag gaacggccac
     4321 gagaacgagc cttttatcgt ggcgatcgta agcatcgtca ggaatatggg caccgtaatc
     4381 acatcgaaaa ccgctgcggc tacgatcagc gcaaggccgg gcttgtgccg caggcgcatc
     4441 cggtgaaccc cgatcgtctg gtagaagccg ctcgcccagc ggcggatctg cgcccgatac
     4501 aacgtccagg tcgccggctc gaccggatac gacatcgccc ggccgttgta tccggccttc
     4561 ccgccctgca actggtgtgc ccacgtcaga tcaaggtctt ccgcgagcgt tcgatccgag
     4621 aatcctccca ggccgcgaac gagcgacgta cgaaacatcg agttgcagcc ggacatcacc
     4681 acgagtgtcc cgagcgcccg ctgaaccggc ttgaagattc ggatcgccat cgagtactcg
     4741 atcgcacgac cgcgggccca aatcccttgc tgcaggagag gcaggcaggc gccactcgtc
     4801 gcatcgagac cacgccgcag gtcgtcggac aggtgctcga ggcactcggg gtggagttgc
     4861 gtgtcggcgt ccaggatcat gaccgcttcg gtgcgcacat cggcgagccc ggcgttgctg
     4921 gctatcgcct tgcttccttg tgcggagccc ggacggatca cgcgggcccc gtgggcacga
     4981 gcgatatctc ccgttccgtc gtcggacgcg tcgtccacga cgatcacctc gtcgggaacg
     5041 atcgactgcg cttcgagcga ccgaagcgtt tcgcccaagt atgcggactc gttgcgggca
     5101 ggtatcagca cggtgatggt cgggcgatcc tgccgcggcg ccttcgacgg gctcgccagg
     5161 ccccggcgga gcgagtggag gcgcgcaccg gcccggcgga gcaagccgat cgttagcggg
     5221 gtaccggtca gccacccggc gcgcaccagc caaaaaagca gcaccgcgct tacgatgacg
     5281 ccggaccccg ggtcgagcgg cgctcgacgg aacatgcgca gccggatggc ggtcccgcga
     5341 tcgccgcggc gcgtcggaac aacgagccct tcgtggtctc ggagacggct ggttgcccgc
     5401 ctgacggtcc cacgccgcag gatcgtgccg gcgcgcagca ggatgatggc catgccgctg
     5461 cgctttccct gggttgaccg ggcgagatag tccagggcgg ccgcggggcc cggccacgga
     5521 tccaaaacga acgagtcggc cagtcctgtg acggcggccg cttcggtaat cgaggggacc
     5581 gcgcaccaca cgtgcagggc gcccgtggcc acgacggtct cgcggagagc ctcacctccc
     5641 gcgccgccga cgacgaatac accgaccgct ccggcagagt ccggttcgac gacatcccga
     5701 tctctggtca cagcacgctc ttgtgaagag cgggcggatc ggagtcctga tgcaacgatg
     5761 cgacgttccc tctccggatc actgtcttct cggcccgacg aagaactgac ggcagcggct
     5821 caggcagcgg ctcgcgctcg acctcgagct ggagctccag ttcggtgttg agcagtccga
     5881 cctcgttctc cagtcgggcg agctcggatg cggcggcgcg aagcagttcc tggacctcat
     5941 gacggtcgta cccgccgact cgccggcgaa gctgggcctg catcagtgct cctcgaatct
     6001 cggccgaggg cggccgccgt ctcattcgcc ggattgacgc cgacgtctgt tcacgctcgt
     6061 tccgtgcatg cgcaagctcg tcctcgagat cggcaacgaa ggccgacacc atcgtgagcc
     6121 atgctcgcac ggcggcgggg tcgaggcctt gacgcgcggt gggaaactcg cgggccgcga
     6181 tctctgatga atgcattctg atactcgtca tgcccgaagg atcgttcacg actgtcacgt
     6241 ggccgtcacc gaggtaccgc attcccaaac cgctcaagcg agctgctcac atcagcaagc
     6301 accaccggca caatgctgcg acaaagcacc ctcgcgacat cgagtgccgg atcgcgcttt
     6361 ggtcttgcgg cctagccgcc gatcatcgac atggagcggc cggggcgttt gaagccgggc
     6421 cggtcgatca aatgcccgcg ccacttcccc gccacgttcg cggtgatcgc ttcgatgatc
     6481 tgatcgtcgc tggcctcgcc gcgcagcatc gcgcgcaggt cggtctcgcc caaggcgaac
     6541 aggcagttgc gcagccgccc ttcggcggtg atgcggaccc ggttgcacga gtcgcagaac
     6601 ggctcggtga cgctgctgat caccccgaca ccgccgggcg caccgtcggc gaatcggtag
     6661 acggtcgcag gctcggggcc gtgctccacg ggctccagcg cgaacgcgtc tctgattcga
     6721 tccagcactt cggcggacgg aacgaccgcg ctacgctccc acgcttcatc ggcgtccagc
     6781 ggcatgaact cgatgaagcg aacctgatag ccggtgtcgc gggcaaagcg cgcgaagtcc
     6841 acgatctcgt cgtcgttggt gccgcggacc acgacggcgt tcagcttgat cggcgccagg
     6901 ccggcttctt ccgcggcctt cagacccttc atgacggcct ccagcgcatc gcggcgtgtc
     6961 atctcggcga agcgatgccg cagcagcgag tccacactga cgttgatgcg cttcagcccg
     7021 gcctcggcca gctccttcgc ttggccggcg agccagaatc cgttggtcgt cagcgacaag
     7081 tccggaacgc cgcgatcgtt gagcatgccg accaaacggg acaggtcgcg tcgcatgagc
     7141 ggctcgccgc cggtcagccg gatcgtcttc acgcccagcc cgacgaagat gcccgcgagc
     7201 cggtcgatct cttcgaaggt caggatctcg tcgcgcggca gccacttgag gccttcggcc
     7261 ggcatgcagt acgtgcaccg aaagttgcac ttgtcggtga cgctgatgcg caggtcgtcg
     7321 gcaacgcggc cgaagccgtc cacgaggctc attcgcccag tataggggtg gccagcaatc
     7381 gccggatccg gcttgccgtt tcggccggat gctcgtcgac gtccgtccat gtgatctgca
     7441 ggacccgcca gcccgccgcc gtcagcagat tccgccgctc gcgatcgctc tcccacgagc
     7501 gccgcccgtt gtgatggcga tacccgtcgg cttcgatcgc gaccttcgct tgcggatacg
     7561 cgaagtccag gcgcatccgg cggccgccca accgcagcgc atgctgatga gtcggttgcg
     7621 gcaggccgtg gtcgtgcacc agacgaacga acatggtctc gagcgggctt tcgggaacgg
     7681 tcacgccggc aggccgggcc gcgagcaggt cacgtatcgc cggcccgccg cgtatccggt
     7741 cccgctcgat cacccgccgg atctgagcgg ccgtcacgag ccgtcggtgc agcgcgtcgt
     7801 ccagcgcgat ctcgaccgaa tcgagcggct gggttgcggc gaggtccaca agggtgcgcg
     7861 ccacggtggt gaccgggatg ccatcgacgc gggtgacctc ggacgcggga agggatctca
     7921 cgcggtgcac ggtgaaccgc cggtgccgca cgccgctgcg cgcggcggtg atctctatcc
     7981 tcgattcctt gaagcccgcc agcgaccgaa gcgccgcggc cgcggtgtgg gacgcgaccg
     8041 catccgggcc cgccgcaagg cacgcggcca ggagcgtctg ccgccacgac ggcggattcc
     8101 cctcgacgac gtagaccccg gggaagatca gccgccatcg tcccagcgcg agccgccgct
     8161 ggatggcgct tgcggacgcg ccggcggcga gcgcctccgc gcgagagacg acgccgtggc
     8221 ggcgggacgc gcgccgggcg agcgtcacgt cccaccgttc gttggtcatt tgactccgaa
     8281 tagcgaactc atatgaccaa caaagcacga aggcagccga tgcccaagcg ccaagcgccg
     8341 cgttcgcggt gcttcggaaa agaaagcgcc acggccggtt cggccgtggc gctccgatcg
     8401 agcggttagt gcacgggcca gatcgcccag gcgaagagca tcccgaccgt cgccgccgcc
     8461 gccacgcgtc cgatgagcga ccagacgcta cgacgcctcc cgatgttggc cggaacgccg
     8521 ctcaccgtcg gggccggttg cggttcggtc gcggccggaa gatccggcgc gatcagaacc
     8581 tgcaccggcg acggcctcgg caagcgcacc accttcgcga cggagccctc cgccggaggc
     8641 gctgcttcgg gcaccggcgt ggctgccgca cgcatcacgc gcgccaggtt caccggcctc
     8701 cccagccgtc tcgcaaccgc cgctcgggcg cctgcgatga tcggctgcac ttgcgcgggc
     8761 cgcacgccca acacgcgtgc gacgtcgctc tcgctcatct tcaggccgag ccgcatcagc
     8821 acggccgctc gctgacgatg tcgcagcgcg atcgctgcgc cgagcatctc gcccggcgag
     8881 tccgctgcgg cgcgaacggt tgcggcttcc gccttcaagg cctgataggc gagcctgagc
     8941 ccgagcacct cctcctcgtc cggccgtgcc ctgcccgcgt gcacgtaccg agctgcctcg
     9001 gccagcgccc acagaagggt tgcccggacg ccgagaggaa gctgttgcgg cgcccgcccc
     9061 accagagcgg aaccgaagaa ccgatacgac cacggcagga cctgctggga ccggactgcc
     9121 ggtcccgacc ggacctgtgt cacgtggagc gctgaatcca tgacctcccc tttctcgggg
     9181 acggatgaga tcggcacgct ccgcttcgct atgcccccga cccggggaca gcaagcaaca
     9241 gtagccccga agggcggcag gtcaaggagg ttcgtggggc tgggccttgg cggcccgctc
     9301 gcggcggttc gtggggctgg gcctcggcgg cccgctcgcg gcggttcgtg gggctgggcc
     9361 ttggcggccc gctcgcggcg gttagtggg
//
LOCUS       DATLFO010000135        13893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_23691, whole genome shotgun sequence.
ACCESSION   DATLFO010000135 DATLFO010000000
VERSION     DATLFO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23691"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VM841_05030"
     CDS             <1..319
                     /locus_tag="VM841_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89582.1"
                     /translation="RTPPQNELAVVFPPPPAPVVAPAPPGAPGVGRKEEHEVEFETES
                     HDDQKHSFTAVRMHQRKDQEAIDRAWTMFGAALVLSLGGAAAAAVYRRRSVVPHHERV
                     RRI"
     gene            319..1602
                     /locus_tag="VM841_05035"
     CDS             319..1602
                     /locus_tag="VM841_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVL89583.1"
                     /translation="MKRALLACVLMLTMVGAITPAVADGHDNYDNVDRLFENSKFCDK
                     FQSFRPREAVVDGPSSQLGLFNEVFEPAYAKACRIGSGLLTYLGTGERAGVNAILNRR
                     ADRAFGTTDVPLSQVEIVEANLDLRAPDRRGRITSIHHIPVAVQITAVVFNLSACGVD
                     QLNLRSPVLSSVFSGTITRWNDGVLVRDNPKLADCDAPIRLVKRADVAGSSLSLKDYL
                     SKRNPQWNYYRQPSRNQAWPTIQNYCPALDEGGMADCIRSTQYSIGYIQYRAARLARL
                     KVARLDNPVSTASLDPDTAFVAPSPAACTAAAESAFIPPGHQKRAVTATFFTFETPSV
                     SPTRGDWNSVSLTDAPRGYPLCSLSYALIYISLQNTYHGQLYLPGTARTAVDYLWTAV
                     SPAAQKRLPAFDFGTLPDQIVAVSRLGLSEIRYAG"
     gene            1804..3126
                     /locus_tag="VM841_05040"
     CDS             1804..3126
                     /locus_tag="VM841_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89584.1"
                     /translation="MSRRARLVAAAFAAVALFGSFARAETVSILSTATNEAVASTSEW
                     GMYLPAGGSTTYSYQIVAKQGQTCVATSANPVHVTVIAPAEIDVTYPAAPQESDPENA
                     LTFRSCGAGQTVTIGGDTIGSFDITALADNVDVDPAKAGYTVHVLQSGSTDAVGDGVI
                     VVDSYTDYRYQALPTQVPNSDGRSLACTLADEDGKRLVLALSAPAEIELQEPVVQDPE
                     NPITPDPVSELTFTSCNNATTVRFASSTPGTYQVTASVVEAPVGFDQSPTTVTVDVVA
                     PVPAQNLNVTIDTPGGRLWSVPAMPGGLPAQTVGPIRVDSVKVGPVTIGPAEVGVSVT
                     VGPVDGPKYPDAMVENITGTASFDRLDGSGVVVQFIGRNVQGEEVEMYTSPAQITCDV
                     NLACTWSLRVPFVLPPGDYRIEATASQANAATSKIRDSKKVTVNATVL"
     gene            3274..5865
                     /locus_tag="VM841_05045"
     CDS             3274..5865
                     /locus_tag="VM841_05045"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL89585.1"
                     /translation="MRRRIALCLVVLLAGIASGVARADHFDSNAGTGSFNPQLQVTVS
                     QSGSPLLVNRPTSFTLKMTQTDHEDPPVVTRLRTPGDWNYAFSSTRQAENSLNVPTTR
                     CTDAIDNRNNSPNGTGTGTQLDFSKRTDAFFARAERIGTGRLLVHADGVSRPGDPILW
                     NAQLAFISYDAVADTAQMCALFITNDPRVTQLPDPNPANTRDDIEGVTEIVGQFPVAR
                     VTEGGVAYWETLVDLTDLIKDSTLQELKVSVLELTSSFTAQSVGNWHAGGITFSRGSV
                     VSGTKSFQGSFKTCPADDPSFGSCKSGRPDVTRTSTFAFTLPAPAITAPASGTLTNVN
                     PVSVSGTADPGASVQVFSGSNAAGSPATADGAGAWTASVPLADGTHSLTAKTVDAGGA
                     SPASNSRTVTVDTTPPAAPVIVSPAEGQLHGGTAVTISGTAEPLSTVQITEAGLPLAT
                     VVSTGTWSTTVNLTKGPHAIEARATDPAGNAGASGPLRSFDVTTAAPVVSSPAQNGAT
                     NATTVNIGGVAEASALVEIAQDGAPLGSTLASTTGTWTFPHSFPEGTHSLTITATAGG
                     FVSPVVTRTFTVDFTPPEAPVFTAPEPGSTVRSISVPIRGTMPAAEVGASVAVFEGGT
                     RLGTALVDATGGWAMTVILQDGLHRVRAIATDRAGNTGAPSALLSFTVDDPLEITHPA
                     ANSFNPGTIEMQGGADPSATQVRLFEGPAEIARVPVVDGAWTATVSFATGTHTVYARA
                     VNNGILGPASPGVTFKVDATPPTVKVKKPQGYTLFGLLAGSPVQGWATDEGPADSRLD
                     RVEVEYRNSLTGETVTRVASCTGCGGPSATWTESQRVLPGFYSVKATAYDKVGNATSE
                     TLLFLIV"
     gene            complement(5855..9646)
                     /locus_tag="VM841_05050"
     CDS             complement(5855..9646)
                     /locus_tag="VM841_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL89586.1"
                     /translation="MATNHRLLPYVPRLALEWMHGGGDVTCLALPGSMVFVDISGFTK
                     MSERLARRGKIGAEEVTEVMNATFSGLLTVAYEHGGGLLKFGGDALLLFFRGSEHPAR
                     AVRAAARMRGRLREVGRVQTSAGLVTLRMSVGVHTGDFHFFLAGREHRELIVTGPAAS
                     RTVEMESAAEAGDVLLSPECAALLPSRHRGKAKGPGILLRGEPPAPDAGEFSDPPLEG
                     IELSDCVPAALRQRLLSGIGEPEHRSASIAFVHFDGTDEMITRDGMETVADRIDELVL
                     AAQQAAAASGVTFLATDIDRDGGKIILVAGVPEARGEDEERILRAVRAIADAKCALPV
                     RIGVNRGPIFAGDVGATYRRTYTVMGDAVNLAARLMAKAAPGQIIASPSVLDRSRTLF
                     ATTALEPFTVKGKAKPIHALDVGAIAGTRAETNRELPFVGRDRELALIADAIASARAG
                     RGTVVDLVGEAGIGKTRLVGEIRRMAADMRFIQTAGEAYAASTPYFAFRRLLRELLDI
                     HPDADPRENTRALTDRIDPVAPELLPWLPLIAVPLDVAVEATPEADAIDSSFRKGRIH
                     DAVSDLLAALLPEPTILVFEDAYWLDDASRELLLHLESTRADSPWLVVPTRRPYTPGF
                     EPERGMSCTLIKLEPLGESESATLAAGAMGDTPITPQQMRVLAERAGGNPLFLQELVA
                     TTAAGASFDALPDSIDAVVTARIDTLDPHDRTLLRYAAVLGSVFTPALARDVLAGEPI
                     ETNEAAWSRLGDFLVHEDEGRYRFLHAMFRDVAYEGLPFRRRRALHRRTGEILEARDT
                     PERFAELLSLHFDRAGDFDKSWRYSSMAGDGAAAKYANIDAARFYRRALDAARRLGTI
                     PDGEVARVAEALGDVSELAAMYPEAQAAYAQARRLTRVAGQDRAGLLMKEGVLRERAG
                     KYADALRWYARGIRTLRDAAAPRGTAAKLTLASAGVRLRQGRFADCVRLCEQVVAANP
                     EPERLAHAYYLLHLAHTSTGSPARARYRTLALPIYEQLGDFMGMANVLNNLGIDAYYE
                     GRWDEATRLYRRGREAREHVGDAVGAALLDNNSGEIYLDQGRLDDAEPLFRQALAVTQ
                     SSGYRVGALLMRSNLARVAARRGETTSALNDLAETAELATEMNAEWFAVQFRTWLAEC
                     AVIGGDHETAMTVAEDAMKAAGNEAGSAVMRAALHRWIGYALAQASRYAQAQDAVDES
                     IMIARASGASFEEALSHEAWVRIAGLTGNDAERDEHDAEARAIFERLGVVWTPSVPLT
                     R"
     gene            10001..>13893
                     /locus_tag="VM841_05055"
     CDS             10001..>13893
                     /locus_tag="VM841_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89587.1"
                     /translation="MNTGRSRAAFAIALALLAPFGSGIRAVDASIDQTVAISIVPSTG
                     ETASGPSAAGAGAVDAANRRVVFVSDASNIVGGDTNGATDVFVHDRISKITSRVSVAT
                     GGAQANGPSSDAVISADGRFVVFRSSASNLVESDTNGADDVFRHDLSSGETIRVSVAD
                     ASGGQANGAAIGSVAVSGTGNAVAFSSLASNLVAGDTNGLSDVFVWRSNGSVVRASLS
                     ATGAQATAGSDSPAISVDGAVVAFRTTAALVPADTQSPAADDVYVRDLIAGTTERVSV
                     SSAGGQGNGVAGAPVIAAAGRFVAFSSTSSNLVPDDANGAADVFVRDRALGDTSRVSV
                     SSAGTEGNGSSGPAAISNDGRLVAFSSSASNLVPGDTNGVSDVFVRDRFLGATARASV
                     GPAGTQGNGASYGQPALSPDGSLIVYTTVAADLAPGDTNEASDVLAGVVPPAAAPAAL
                     RLASINSSFGRANGASELSSLSGDGRFVAFASDASNLVPGDTNGTGDVFVRDAVTGFV
                     ERVSVSNSGAQGDGRSGEPSISADGRFVAFYSAATNLVPNDTNGPGLSGDDIFLRDRL
                     TATTTRISVNNLGIQVGGPSRAPKISADGSYIVFQSDAIDLTGPDLNNATDVFRLNRL
                     TGQVEMVSVSTAGAQGNGASTGPSISEDGGVVAFHSAASNLVPGDTNAGTDVFVRTFA
                     PRATTRVSVATGGAQGIAGNAAAGVVSATGRSVAFHASYPNLVAGDTNGVADVFLHDR
                     TSGATSRISLASDGEQSNGVSVLQSREQISADGRFVVFESGATNLVAGDSNGVTDVFV
                     RDVVAGTTTRLSESAAGSGGSATSQLPAISFDGRVVVFASDAADLVASDTNGRRDLFV
                     SQRAVEGTAAASVSSSNVVASGTQTAGPAGRSISDDGRFVVFASDAANLVAGDTNGVA
                     DIFVRDRITGATRRVSVSSAGAQATLASSEPAISASGRFVVFTSQAPNLVAGDTNGTA
                     DVFRADLLSGDVRRVSTLADGELAGPSGRAVISADGRFVAFESSAANIPGDTNPGADV
                     VVKDVSAGTLAVASAGANAPATRPSISDDGSVVAFVSEASTLVAGDTNGAADAFAWTR
                     ATGAVSLISLADSGQRADGPTSAVALSRTGRFAAFASTATNLAPGRPGIFLRDRTLGS
                     TDLVSGADADGGSREPAISSEGRFVAFVSDAANLVTGDTNGAADVFVADTVLGGFRRA
                     SAGSFGQQAATASRAPSIAANGAVTVFETASSNVAGYDRNGASDVFARALSFAPAPVS
                     TTIRNLSIAVNDFSIAAGVPSIPIGDISSGRVVSL"
BASE COUNT         2055 a         5005 c         4715 g         2118 t
ORIGIN      
        1 ccgcaccccg ccgcagaacg agctcgccgt tgtgttcccg ccgccgcccg cgccggtggt
       61 ggctccggct ccgccgggcg ctcccggcgt cggccgcaag gaagagcacg aggtcgagtt
      121 cgagaccgag tcgcacgacg accagaagca ctcgttcacg gcggtgcgca tgcatcaacg
      181 caaagaccag gaagcgatcg accgggcatg gacgatgttc ggtgcggcgc tggtgctgtc
      241 gctgggcggc gccgcagccg cggccgtcta ccggcgcagg tccgtcgtgc ctcatcacga
      301 gcgagtcagg aggatctgat gaagagggct ctgctcgcgt gcgtgctcat gctcacgatg
      361 gtgggcgcga tcacgcctgc cgtcgccgac ggtcacgaca actacgacaa cgtcgatcgc
      421 ctcttcgaga actcgaagtt ctgcgacaag ttccagtcgt tccgtcctcg tgaggcggtc
      481 gtggacggac cgtcctcgca gctgggcctg ttcaacgagg ttttcgagcc cgcatacgcc
      541 aaggcgtgcc ggatcgggtc gggcctgctg acctatctcg gcacgggcga gcgcgccggt
      601 gtgaacgcga tcctgaaccg ccgggcggat cgcgcgttcg gcaccacgga cgtcccgctg
      661 tcgcaggtcg agatcgtcga ggcgaacctg gaccttcgtg cgcccgaccg gcgcggccgc
      721 atcacctcga tccaccacat tcccgtggcg gtgcagatca ccgcggtggt gttcaacctg
      781 tccgcctgcg gcgtggatca gcttaacctc cgcagcccgg tgttgagctc ggtgttctcg
      841 ggaacgatca cgcgctggaa cgacggcgtt ctggtccggg acaacccgaa gctcgcggac
      901 tgcgacgcgc cgatccgtct ggtcaagcgc gcggacgtcg ccgggtcctc gctgtcgctc
      961 aaggactatc tgtcgaagcg gaacccgcag tggaactact accggcagcc gtcccggaac
     1021 caggcgtggc ccacgatcca gaactattgc ccggcgctgg acgagggcgg catggccgat
     1081 tgcatccgct cgacccaata ctcgatcggg tacatccagt atcgcgcggc gcgtcttgcc
     1141 cggctgaagg tcgctcgcct ggacaacccg gtgagcacgg cctcgctcga cccggacacg
     1201 gccttcgtgg ccccgagccc ggcggcctgc accgccgctg cggagtcggc ctttatcccc
     1261 ccggggcatc aaaagcgagc ggtcacggcg acgttcttca ccttcgagac cccgtcggtg
     1321 tcgccgaccc gcggagactg gaactcggtc tcgctgacgg acgctccccg gggctatccg
     1381 ctttgctcgc tcagctacgc gctgatctac atctcgctcc agaacaccta ccacgggcag
     1441 ctctacctgc cgggaacggc caggaccgcg gtggactacc tctggacggc ggtgagcccc
     1501 gccgcccaga agcggctgcc ggccttcgac ttcgggaccc ttcccgacca gatcgtggcc
     1561 gtctcgcgcc tgggcctgag cgagattcgc tacgcgggat aagccggaga ttctctccat
     1621 ttcctcaccg cgacccggaa ttggctcact cccccagcag gattccagac tgtatgccca
     1681 gaatcatgca catgatgcag taggtccggt tcatcctggg ccgatgggtc ggcttcccca
     1741 tccgggggcc gacggggagg gtgatcgttg catggatacg gtcgtttcca taggggggag
     1801 atcatgagtc gtcgggcacg tttggtagcg gccgcgtttg ccgcggttgc attgttcggc
     1861 tcgttcgcgc gagccgagac cgtttcgatt ctctcgacgg cgaccaacga agccgtcgcc
     1921 agcacgtccg aatggggcat gtacctgccc gccggcggct cgaccacgta ctcgtaccag
     1981 atcgtggcca agcagggcca gacgtgcgtg gccacgagcg cgaacccggt gcacgtcacc
     2041 gtcatcgcgc cggccgagat cgacgtcacc tacccggctg cgccgcagga gagtgacccc
     2101 gagaacgcgc tgaccttccg tagctgtggc gccggccaga cggtcaccat cggtggcgac
     2161 acgatcggca gcttcgacat cacggccctg gccgacaacg tcgacgtcga cccggcgaag
     2221 gccggctaca ccgtgcacgt ccttcagagc ggctcgaccg acgcggtcgg cgacggcgtc
     2281 atcgtcgtcg acagctacac cgactaccgc taccaggcac tgcccacgca ggttccgaac
     2341 tccgacggcc gctcgctggc gtgcacgctc gccgacgagg acggcaagcg cctggtgctc
     2401 gcgctgtccg ccccggccga gatcgagctt caggagccgg tcgtgcaaga ccccgagaac
     2461 ccgatcacgc ccgacccggt gagcgagctc accttcacca gctgcaacaa cgccaccacc
     2521 gtgcgcttcg cctcgtcgac gcccggcacg taccaggtga ccgcgagcgt cgtggaggct
     2581 ccggtcggct tcgaccagtc gcccacgacc gtcaccgtcg atgtcgtcgc gccggttccc
     2641 gcgcagaacc tgaacgtgac catcgacact ccgggcggtc gcctgtggag cgtgccggcg
     2701 atgccgggcg gcttgccggc ccagacggtc ggtccgatcc gcgtggacag cgtcaaggtc
     2761 ggccccgtca ccatcggccc ggccgaggtc ggcgtgtccg tcacggtcgg tccggtcgac
     2821 ggacccaagt acccggacgc gatggtggag aacatcaccg gcacggccag cttcgaccgg
     2881 ctcgacggca gcggtgtggt cgtccagttc atcggccgca acgtgcaggg tgaggaagtc
     2941 gagatgtaca cgtcgcccgc gcagatcacc tgcgacgtga acttggcgtg cacatggtcg
     3001 ctccgcgtgc cgttcgtcct tccgccgggt gactaccgga tcgaggccac cgccagccag
     3061 gcgaacgccg cgacctcgaa gatccgcgac tcgaagaagg tcacggtcaa cgcgaccgtc
     3121 ctgtagggac accgaacgcc ggaaagaccc ccgccgcgcc gggggtcttt ccttttcccc
     3181 ttgaaccggg tcgcggccgg gaaggaaccg atccggtcaa aggcgaagta gcacgtccag
     3241 ggaagggttt ggcacgaaaa gggagtgttt tccatgcgtc gacgcatcgc gctctgcctc
     3301 gtcgtcctgc tcgccgggat cgcctccggc gtcgcgcgcg ccgatcattt cgacagcaat
     3361 gcgggcaccg gctcgttcaa cccgcagctt caggtgaccg tgagccagtc cggctcgccg
     3421 ctgctggtga accggccgac gagctttacg ctcaagatga cccagaccga ccacgaggat
     3481 ccgccggtcg tcacccggct gcgcacgccc ggcgactgga actacgcgtt ctcgtccacg
     3541 cggcaggccg agaactcctt gaacgtgccg acgacgcgat gcaccgacgc gatcgacaat
     3601 cgcaacaact ccccgaacgg caccggcacc ggaacgcagc tcgacttctc gaagcgcacg
     3661 gatgcgttct tcgcgcgagc ggagcggatc gggaccggcc ggctgctcgt gcacgccgac
     3721 ggcgtctcgc gtccaggcga cccgatcctg tggaacgcgc agctcgcgtt catctcctac
     3781 gacgccgtgg ccgacaccgc gcagatgtgc gcgctgttca tcaccaacga tccccgcgtc
     3841 acccagcttc cggacccgaa tccggctaac acccgcgacg acatcgaagg cgtcaccgag
     3901 atcgtcgggc agttcccggt cgcacgcgtg accgagggcg gcgtcgccta ctgggagacg
     3961 ctcgtcgacc tcaccgatct gatcaaggat tccacgctgc aagagctcaa ggtcagcgtg
     4021 ctcgagctga cctcgagctt cacggcccag tcggtcggaa actggcacgc gggcggcatc
     4081 acgttctcgc gcggctcggt cgtgtcggga acgaagtcgt tccagggcag cttcaagact
     4141 tgtcccgccg acgacccgtc gttcggcagc tgcaagagcg gccggccgga cgtgacccga
     4201 acctcgacgt ttgcgttcac gctgccggct ccggccatca ccgccccggc ttcgggaacg
     4261 ctcaccaacg tcaacccggt ctcggtgagc ggcactgccg acccgggcgc ctcggtgcag
     4321 gtcttctcgg gatccaacgc ggccggctcg cccgcaacgg ccgacggcgc cggcgcgtgg
     4381 accgcgtcgg ttccgctcgc cgacggcacg cacagcctga ccgccaagac ggtcgacgcc
     4441 ggcggcgcct cgcccgcgtc gaacagccgc accgtgacgg tggacaccac gccgccggcg
     4501 gcgccggtga tcgtctcgcc cgccgaaggt cagctgcacg gcggcaccgc ggtcacgatc
     4561 agcggaacgg cagagcctct gtccaccgtc cagatcaccg aggcgggcct gccgctggcg
     4621 accgtcgtct cgaccggcac ctggtccacg acggtgaacc tgacgaaggg tcctcacgcg
     4681 atcgaggccc gggcgaccga tccggccggc aacgcgggcg cgtccggccc gctgcggagc
     4741 ttcgacgtca ccaccgccgc gccggtcgtg tcgtcgccgg cgcagaatgg ggccaccaac
     4801 gccacgacgg tcaacatcgg cggtgtggcc gaggcgagcg cgctcgtcga gatcgcccag
     4861 gacggcgccc cgctcggatc gacgctggcg tcgaccacgg gcacctggac cttcccgcac
     4921 tcgttccccg aagggaccca ctcgctgacg atcaccgcca cggcaggcgg gttcgtctcg
     4981 ccggtcgtca cgcggacctt cacggtggac ttcacgccgc cggaagcgcc cgtctttacc
     5041 gcgcccgagc ccggctcgac cgtccggtcg atcagtgtcc cgattcgcgg caccatgccc
     5101 gccgccgagg tgggggcgag cgtggccgtg ttcgagggcg gcacgaggct cggcaccgcg
     5161 ctcgtcgatg cgacgggcgg ctgggcgatg accgtgatcc tgcaggacgg cttgcaccgc
     5221 gtgcgcgcga tcgcaaccga ccgcgcgggc aacaccggcg ccccgtcggc gttgctgagc
     5281 ttcaccgtgg acgatccgct cgagatcacg catccggccg cgaacagctt caaccccggc
     5341 acgatcgaga tgcagggcgg cgccgacccg tcggcgacgc aggtccggct gttcgaaggc
     5401 cccgcggaga tcgcgcgcgt cccggtcgtg gacggcgcct ggaccgcgac cgtgtcgttc
     5461 gcgacgggca cgcacaccgt ttatgcccgt gcggtcaaca acgggatcct cggtccggcg
     5521 agccccggcg tgacgttcaa ggtcgacgca acgccgccca cggtcaaggt caagaagccg
     5581 cagggctaca cgctgttcgg gttgctggcc ggcagcccgg tacagggatg ggcgaccgat
     5641 gagggtccgg cggattcgcg gcttgatcgc gtcgaggtcg agtaccggaa ctccctgacc
     5701 ggcgagacgg tgacgcgcgt cgcgtcctgc accggctgcg gcgggccgag cgcgacctgg
     5761 accgagtccc agcgcgtgct gccggggttc tactcggtga aggcgaccgc ctacgacaag
     5821 gtcggcaacg ccacatccga aacgctgctg ttcctcatcg tgtaagcggc acggaaggcg
     5881 tccacacgac gccgaggcgc tcgaagatcg cgcgcgcctc ggcgtcgtgc tcgtcccgct
     5941 ccgcgtcgtt tccggtcagc ccggcgatgc ggacccaggc ctcgtgcgac agggcctctt
     6001 cgaacgaggc acccgaggcg cgcgcgatca tgatcgactc gtccacggcg tcctgcgcct
     6061 gggcgtagcg gctcgcttgc gccagcgcgt atccgatcca gcgatgcagc gcggcgcgca
     6121 tcaccgcgct gccggcctcg ttgccggcgg ccttcatcgc gtcctcggcg accgtcatcg
     6181 ccgtctcgtg atcgccgccg atgacggcgc attcggccag ccacgtccgg aactggaccg
     6241 cgaaccattc cgcgttcatc tcggtcgcga gctcggcggt ctcggccagg tcgttgagcg
     6301 ccgaggtggt ttcgccgcgc cgggcggcca cgcgcgcgag attcgatcgc atcagcagcg
     6361 ccccgactcg atacccgctc gattgcgtga ccgccagcgc ctggcggaac agcggctcgg
     6421 cgtcgtcgag gcggccctgg tccaggtaga tctcgccgct gttgttgtcc agcagcgctg
     6481 ctccgaccgc gtcgccgacg tgctcgcgcg cctcgcgtcc gcggcggtac agcctcgtgg
     6541 cctcgtccca gcgcccttcg taataggcgt cgatgccgag gttgttcagg acgttggcca
     6601 tgcccatgaa gtcgccgagc tgctcgtaga tcggcagcgc gagcgtgcgg taccgggccc
     6661 gcgcggggct gccggtcgag gtgtgggcca ggtgcagcag gtaatacgcg tgagcgagcc
     6721 gttcgggttc cgggttcgcg gcgacgacct gctcgcacag ccggacgcag tcggcgaagc
     6781 ggccctgccg caggcggacc ccggcggacg cgagcgtgag cttcgcggcc gtcccccggg
     6841 gggccgcggc atcccgcagc gtgcggatcc cgcgcgcgta ccaccgcaag gcgtccgcgt
     6901 actttccggc gcgctcgcgc agcacccctt ccttcatcag caggccggct cggtcctgac
     6961 cggcaaccct tgtgagccgc cgcgcctgcg cgtaggcggc ctgcgcctcg gggtacatcg
     7021 ccgcgagctc ggacacgtcg cccagcgctt cggcgacgcg ggcgacctcg ccgtcgggga
     7081 tggttccgag gcggcgcgcc gcgtccaagg cgcggcggta gaaccgggcc gcgtcgatgt
     7141 tggcgtactt ggcggccgcg ccgtcgccgg ccatcgagga gtagcgccac gacttgtcga
     7201 agtcgccggc gcggtcgaag tgaagggaca gaagctcggc gaagcgctcc ggcgtgtcgc
     7261 gggcttcgag gatctcgccg gtgcggcggt gcagcgcgcg ccggcggcgg aacggcaagc
     7321 cttcgtaggc gacgtcgcgg aacatcgcgt ggaggaaccg gtatcgtccc tcgtcttcgt
     7381 gcacgaggaa gtcgccgagc cgcgaccaag ccgcttcgtt cgtctcgatc ggctcgccgg
     7441 cgagcacgtc ccgggccagc gcgggggtga agaccgagcc gagcacggcg gcgtagcgca
     7501 gcagcgtgcg gtcgtgcgga tcgagcgtgt cgatgcgagc cgtgacgacc gcatcgatcg
     7561 agtcgggcag cgcgtcgaag gatgcgcccg ccgcggtggt cgcgacgagc tcttgcagaa
     7621 acagcgggtt gccgcccgca cgctcggcga gcacgcgcat ctgttgcggc gtaatcgggg
     7681 tgtcgcccat cgcgcctgcc gcgagcgtcg ccgactcgct ttcgccgagt ggttcgagct
     7741 tgatcagggt gcagctcatg ccgcgctcgg gctcgaatcc cggcgtgtac gggcggcgcg
     7801 tcggaacgac cagccatggg ctgtcggcgc gagtcgattc cagatgcagc aagagctccc
     7861 gcgacgcgtc gtcgagccaa taggcgtcct cgaagaccag aatcgtgggc tcgggcagca
     7921 gggccgcgag cagatcactg accgcgtcgt ggattcgtcc cttgcggaag gacgagtcga
     7981 tcgcgtccgc ctcgggcgtc gcctccaccg ccacgtccaa ggggaccgcg atgagcggca
     8041 gccacggcaa gagctccggg gcaaccgggt caatgcggtc ggtgagcgcg cgggtgttct
     8101 cgcgcggatc cgcgtccgga tggatgtcca gcagctcgcg aagcaggcgg cggaacgcga
     8161 agtacggcgt cgaggcagcg tacgcctcgc cggcggtctg aatgaagcgc atgtccgcgg
     8221 ccatgcgccg gatctctccg acgaggcgcg tcttgccgat gccggcctcg ccgacgaggt
     8281 cgacgacggt tccgcgcccg gcgcgcgccg aggcgatcgc gtcggcgatg agcgcgagct
     8341 cgcgatcacg ccccacgaac ggcagctcgc gattcgtttc cgcgcgcgtg ccggcgatcg
     8401 cccccacgtc gagcgcgtgg atcggcttcg ccttaccctt caccgtgaac ggctcgagcg
     8461 ccgtcgtagc gaacagggtg cgggaccggt cgagcacgct cggcgaggcg atgatctgac
     8521 cgggcgccgc tttggccatc agccgcgcgg cgaggttcac cgcgtcgccc atcaccgtgt
     8581 acgtgcgccg gtaggtcgcg cccacgtcgc ccgcgaagat cggtccgcgg ttcaccccga
     8641 tgcggaccgg cagcgcgcat ttcgcgtcgg cgatggcgcg cacggcgcgc aggatgcgct
     8701 cctcgtcctc gccgcgcgcc tcgggaacgc ccgcaacgag gatgatcttg ccgccgtcgc
     8761 ggtcgatgtc ggtggcgaga aaggtgacgc cgctggccgc ggccgcctgc tgtgccgcga
     8821 gcacgagctc gtcgatccgg tccgcgacgg tctccatccc atcccgcgtg atcatttcgt
     8881 cggtgccgtc gaaatgaacg aacgcgatcg atgccgaacg atgctccggc tcaccgatgc
     8941 ccgacagcag ccgttggcga agcgcggccg gcacgcagtc cgagagctcg atgccctcaa
     9001 ggggcgggtc ggagaactcg cccgcgtccg gggccggggg ctcgccgcgc agcaggatcc
     9061 cgggcccttt ggccttgccc cggtggcggg acgggagcag ggcggcgcac tccggagaca
     9121 gcaggacgtc gcccgcctcg gccgccgact ccatctcgac cgtccggctg gccgccggtc
     9181 cggtcacgat gagctcgcga tgttcccggc cggcgaggaa gaagtggaag tctccggtgt
     9241 gcacgccgac cgacatgcga agcgtaacca ggccggccga cgtctgcacc cggccgactt
     9301 cgcggagccg tccccgcatg cgggccgccg cgcgcacggc tcgggccgga tgctccgagc
     9361 cgcggaagaa cagcagcagc gcgtccccgc cgaacttgag caggcccccg ccgtgctcgt
     9421 aggcgaccgt cagcaggccc gagaaggtcg cgttcatgac ctcggtgacc tcttcggcgc
     9481 cgatcttgcc gcggcgggcc agccgctcgg acatcttggt gaagcccgag atgtccacga
     9541 agaccatcga tcccggcagt gcaagacagg tgacgtcccc gccgccgtgc atccattcca
     9601 gggccaggcg cggaacgtag gggagcagcc gatggttggt tgccactcgt gcgctcgtgg
     9661 tcaccgagag tcctccgcca gtgctgctga caatggaagc aagcaattcg agcgccggga
     9721 cccggccccg caccggggcc gcagcggggc cggcgtcgta ggaaaacgac tgtactggac
     9781 acttcgttta ggaactacga ctgcataatc gaaaaaatgg agccctttgt aggatcaaag
     9841 tggacatagg gctcaaatca gaagtgccgg atgtgacgcc cgtcacggat aattcgtaac
     9901 ggatcgaata ggtggctcgt tcgatcccga cgaaggttac gtcgtaccgg ccgtctcgcg
     9961 aggggcggtc gcccgcagag cgacgatgag ggggactctc atgaacaccg gccgttcgcg
    10021 cgccgcgttc gcgatagcgc tggcgctgct tgcgcctttc ggctccggca tccgcgccgt
    10081 cgatgcatcc atcgaccaaa ccgtggccat ctcgatcgtg ccctccaccg gggagaccgc
    10141 gtccggaccg agcgccgcgg gggcgggggc ggtggacgcc gccaatcgcc gcgtcgtgtt
    10201 cgtgtccgac gcgtccaaca tcgtgggcgg cgacaccaac ggcgcgaccg atgtgttcgt
    10261 ccacgaccgg atctcgaaga tcacctcgcg cgtctcggtc gcgaccggag gggcgcaggc
    10321 gaacggccca tcgagcgacg cggtcatctc ggccgacgga cgcttcgtcg tcttccgttc
    10381 ctcggcatcc aacctcgtcg aaagcgacac caacggcgcc gacgacgtgt tccgtcacga
    10441 cctgtcgagc ggcgagacca tccgcgtctc cgtcgccgac gcctcgggcg ggcaggccaa
    10501 cggcgcagcg atcggctcgg tcgccgtctc cgggacgggc aacgccgtcg cgttctcgtc
    10561 gcttgcatcc aacctcgtcg ccggcgacac caacggcttg tcggacgtgt tcgtgtggcg
    10621 ttcgaacggc tcggtggttc gggcctcgct gtcggcaacc ggtgcgcagg caacggccgg
    10681 atccgactcg cccgccatct cggtcgacgg tgcggttgtg gcgttccgaa cgacggctgc
    10741 cctcgtcccg gccgacaccc agtcgcccgc ggcggacgac gtctacgtgc gcgacctgat
    10801 cgcgggcact accgagcgcg tctcggtctc gtccgcgggc gggcagggca acggcgtcgc
    10861 gggcgcgccg gtcatcgccg cggctggccg cttcgttgcc ttctcctcga catcgtcgaa
    10921 cctggttccc gacgacgcca acggcgccgc cgacgtgttc gtgcgcgacc gtgcgctcgg
    10981 cgacacctcc cgcgtgagcg tctcctccgc cggcacggag ggcaacggct ccagcggccc
    11041 ggcggcgatc tccaacgacg gccgtttggt cgcgttctcg tcctccgcgt cgaatctcgt
    11101 gcccggcgac accaacggcg tgtcggacgt gttcgtgcgc gaccgtttcc tcggcgcgac
    11161 cgcgcgcgcg tcggtcgggc cggccggaac gcagggcaac ggtgcgtcct acgggcagcc
    11221 cgcgctgtct cccgacggat cgctgatcgt ctacaccacg gtcgccgccg atctcgctcc
    11281 cggcgacacg aacgaggcct ccgatgtgct tgccggcgtg gttccgcctg ccgcggcgcc
    11341 ggcggcgctg cgtctggcga gcatcaactc gtcgttcggc cgcgccaacg gtgcgtccga
    11401 gctgtcctcg ctcagcgggg acggccgctt cgtcgcgttc gcatcggacg catccaacct
    11461 cgtgcccggc gacaccaacg ggaccggaga cgtgttcgtg cgcgacgccg tcaccggctt
    11521 cgtcgagcgc gtctcggtct ccaactccgg cgcgcaaggc gacggccgct cgggcgagcc
    11581 gtccatcagc gccgacggcc gtttcgtggc gttctactcc gcggccacga acctggtgcc
    11641 caacgacacc aacggtcccg gcctgagcgg cgatgacatc ttcctgcgtg atcgcctcac
    11701 ggccacgacg acacggatct cggtgaacaa cctcggcatc caggtcggcg gcccgagccg
    11761 ggcgccgaag atctccgccg acggctcgta catcgtgttc cagtcggacg cgatcgacct
    11821 gaccggcccc gaccttaaca acgccacgga cgtgttccgc ctcaaccgcc tcaccggcca
    11881 agtcgagatg gtctcggtct ccacggcggg tgcgcagggc aacggcgcgt cgacgggccc
    11941 ctcgatctcc gaggacggcg gcgtggtcgc attccattcg gccgcgtcca atctcgtgcc
    12001 gggcgacacg aacgcgggaa ccgacgtgtt cgtgcgtacc ttcgctccgc gtgccacgac
    12061 acgcgtttcg gtcgcgacgg gcggcgcgca aggcatcgcc ggcaacgccg ccgccggcgt
    12121 ggtgtccgcc accgggcgct ccgtcgcctt ccacgccagc tacccgaacc tcgtcgccgg
    12181 agacaccaac ggcgtcgccg acgtgttcct gcacgaccgc acttcgggcg cgacctcgcg
    12241 catctcgctc gcttcggacg gcgagcagtc caacggcgtg tcggtgctgc aaagccgcga
    12301 gcagatctcg gccgacggcc gcttcgtcgt cttcgagtcc ggcgcaacca acctcgtcgc
    12361 cggcgactcc aacggcgtga ccgacgtctt cgttcgcgac gtcgtcgccg gcacaaccac
    12421 gcgcctgagc gagtcggctg caggatcggg cggctcggcg acgtcccagc tgccggcgat
    12481 ctccttcgac ggccgggttg tggtcttcgc ctccgacgcg gcggatctgg tcgcctccga
    12541 caccaacggc cgccgcgacc tgttcgtgtc gcaacgcgcc gtggagggca ccgccgccgc
    12601 gagcgtgtcc tcgtccaacg tggtcgcgtc cggaacgcag acggcgggcc cggccggacg
    12661 ctcgatctcc gacgacggcc gtttcgtggt cttcgcctcc gacgcggcca acctcgtcgc
    12721 cggagacacc aacggcgtcg ccgacatctt cgtgcgcgac cgcatcaccg gcgccacccg
    12781 gcgcgtgtcg gtctcgtcgg ccggcgcgca ggcgacgctg gcctcgtcgg agccggcgat
    12841 ttcggcgagc ggccgtttcg tggtgttcac ttcgcaggcg ccgaacctgg tcgccggcga
    12901 cacgaacggc acggcggacg tgtttcgcgc ggacctgctg tccggggacg tgcggcgcgt
    12961 gtccacgctc gccgatggcg agctcgccgg gccgtccggc cgcgcggtga tctccgccga
    13021 cggccggttc gtcgccttcg agtcttcggc cgcgaacatt ccgggcgaca ccaaccccgg
    13081 cgccgacgtc gtggtcaagg acgtctcggc gggcacgctc gccgtcgcgt ccgccggcgc
    13141 caacgccccg gcgacccgcc cgtcgatcag cgacgacgga tccgtcgtcg ccttcgtcag
    13201 cgaggcatcg accctggttg ccggcgacac caacggcgcc gccgacgcgt tcgcgtggac
    13261 gcgcgccacc ggagcggtct cgctgatctc gctggcggac agcgggcagc gcgcggacgg
    13321 accgacctcc gcggtcgcgc tttcgcggac cggccgcttc gcggccttcg catccaccgc
    13381 gaccaacctc gcgccgggac gtcccggcat cttcttgcgc gaccgcacgc tcggctcgac
    13441 cgatctcgtc tcgggcgccg acgccgacgg tggctcgcgc gagcccgcga tctcgagcga
    13501 gggccgcttc gtggcgttcg tcagcgacgc ggccaatctc gtgacgggcg acaccaacgg
    13561 cgccgccgac gtcttcgtgg ccgacaccgt gctcggcgga ttccgccggg cctcggcggg
    13621 ctcgttcggg cagcaggccg cgacggcgtc ccgtgctccg tcgatcgccg cgaacggcgc
    13681 ggtgaccgtc ttcgagaccg cctcgtcgaa cgtggcgggc tacgaccgca acggcgcatc
    13741 ggacgtcttc gctcgcgccc tgagcttcgc gccggccccg gtgtcgacga ccattcgcaa
    13801 cctgtcgatc gcggtcaacg acttcagcat cgcggcgggt gtgccctcga ttccgatcgg
    13861 tgatatctcg tccgggcggg ttgtgtcgtt gat
//
LOCUS       DATLFO010000136        19355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_45630, whole genome shotgun sequence.
ACCESSION   DATLFO010000136 DATLFO010000000
VERSION     DATLFO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19355
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45630"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VM841_05060"
     CDS             <1..184
                     /locus_tag="VM841_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVL89588.1"
                     /translation="IVAVLIGGTLAGFAGALLALPAAAIIKVILQRVYLHERLAVVQR
                     EDEEHAAANAGSKPTG"
     gene            406..780
                     /locus_tag="VM841_05065"
     CDS             406..780
                     /locus_tag="VM841_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89589.1"
                     /translation="MMLQRANFRLARRRRLTLLTVVFLATLSACIGLITTRHATMTGS
                     AISFLAGDLLVLVWAGILVVPSFAGSVKPRPVRAGALGALLFGPLLMPVVFGPGGWKA
                     WQVAALVAASIAALVPPRSLLR"
     gene            complement(781..1164)
                     /gene="rsfS"
                     /locus_tag="VM841_05070"
     CDS             complement(781..1164)
                     /gene="rsfS"
                     /locus_tag="VM841_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HVL89590.1"
                     /translation="MNSTLAPPKALAIDVARAASAKKAIDIRILEVGSLIEICDYFVI
                     CSGATDRQVRTIVNEIEQAVAPRKPVRREGERERTWVVLDYGDVVVHVFAQEQREYYE
                     IERVWRDAPIIDFEEEDGPAAAARS"
     gene            complement(1186..2442)
                     /locus_tag="VM841_05075"
     CDS             complement(1186..2442)
                     /locus_tag="VM841_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HVL89591.1"
                     /translation="MITEEEMQSSPELDELGFEPGGRTRRNRRRRRRSRAVALLMVMV
                     LAAGAAWAVATWLDRGRGGSGVLAGDKDGQTAILFMIRQADDPTRRSDMLVVFALDAA
                     GTNPITLFMPTNALTEIPGHGLDLAGRGFQFGGMRLQSLVVDNVLGIVLDRDAAMTDA
                     VLGKLVDAAGGLDITVEDTLIEPGGGVAFSPGLQKMNGEAVRGYLRYQGDGETELSRI
                     ARAQHVWEAMFAAWGRLGVGALAEKFREIGSEWNEGIETGLTPLDLAEFFSAFTAGSP
                     ADRIYTALPVEPVSAGGSEQALRIDEDQVGNLVSQYFAASIPKNPYLGTRIEVLNGNG
                     IPQIGEEVALKLIPKGYRIVLNKNARSFDFETTKIVVYSRDERSLGAARKIQELLGVG
                     EIEIGTRAQTVVDVTIVVGHDFEPRD"
     gene            complement(2439..3038)
                     /gene="nadD"
                     /locus_tag="VM841_05080"
     CDS             complement(2439..3038)
                     /gene="nadD"
                     /locus_tag="VM841_05080"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HVL89592.1"
                     /translation="MTRRRIGVMGGTFNPIHSGHLVAADEALVAFDLDEVVFVPAGRP
                     WQKEGIDVAPAEDRYLMTVIATSSHPRFRVSRVEIDRDGPTYTVETLALLKETLGDVD
                     LSFITGADAVLEILSWKDPEEVLRRARFIACTRPGYDLDRLAKQMPEGLEERVDVMEI
                     PALAISSTDIRRRVAERRPIRYLVPEGVATYIEKSGLYR"
     gene            complement(3150..3494)
                     /locus_tag="VM841_05085"
     CDS             complement(3150..3494)
                     /locus_tag="VM841_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVL89593.1"
                     /translation="MTRFAIVAALSLLAGACVGGSDPAAAPTPAGEPATAVTVDSTDK
                     LTYEPARVLLKAGGTVTWTNTGRVPHTVTFSGFDKPLKAGDEVTFTFTTSGTTKVRCT
                     LHPRMTGEIVVV"
     gene            complement(3501..3905)
                     /locus_tag="VM841_05090"
     CDS             complement(3501..3905)
                     /locus_tag="VM841_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVL89594.1"
                     /translation="MARLARPAAFAAILALALVACGDAKDTGLPSGPTPEPVAQTVVD
                     VVDSAFKPAELEVKVGETVTWDFTGSAPHNVKFTTFEEDSHPECEADINKCSKAGEEY
                     EFTFESAGSFPYFCVIHGTANGQGMAGTIVVA"
     gene            4027..5460
                     /locus_tag="VM841_05095"
     CDS             4027..5460
                     /locus_tag="VM841_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL89595.1"
                     /translation="MREELLRIGGVARPGSGRSTIVSPATGEPVASVAVASASDVDEA
                     VRAAQAAFAGPFGALAEHERQAILRRASDLLAERVEDLARTITLENGKPIRDARGEAA
                     RAWQTILFAAEEAKRIHGEVLPMGAARGGEGRIGFTLRKPIGVVAAIAPFNFPLNLVC
                     HKVGPALAAGNTVVLKPSENTPLSSFALAAVFEEAGLPPGALNVVCGDAETGEALVDH
                     PGVAMITFTGSAAVGTRIRSRSGVRRTLLELGSNAPMIVFDDADIGAALEAAVRGSFT
                     YSGQVCISIQRLLVQRPLFDPFVVRLAEAASALVVGDPLDEATHIGPMIDEDAARRVE
                     SWVDAAVAGGARAECGGTRDGRFYAPTVLTGVKPDMDVVCNEIFGPVVSVMPFDGLDD
                     AIAIANDSVYGLQAGVFTRDIDRAMRVAQRLDVGGLWINDAPQTRFDHYPYGGMKSSG
                     LGREGVRYAVEEMTETTFVGIKLGDAR"
     gene            5473..6807
                     /locus_tag="VM841_05100"
     CDS             5473..6807
                     /locus_tag="VM841_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL89596.1"
                     /translation="MGFDDAVRRSKEMLDVVLDGIDSGEAREALAKQTVIDFDEHFTP
                     GLTRYRKSVTEAGDFAAIEWTGEGSIVYDAKGREWIDCLGGYGIYSLGIRNPEVIAAV
                     RAQMDRNPMPTQELLDPFKAMLCALLAEIAPGDMQNVFVANSGTESIEGAMKIARVHT
                     GRPGFVVAEAAFHGKTLGALSLMGKPAFREAVEPLLSPVWRVPFGDAAAVDAILRDHA
                     TQVAAVVMEPIQGEAGAVVPPEDFWPSLRAICDRYGVLLIADEVQTGLGRTGKLWGVD
                     HWDVAPDIITSAKALGGGVMPIGAVAGRAEIWRSLEPNPFLHTSTFGGNPLACAAAIA
                     AIHVILRDQLWERADRIGTRFVEALTDMADRYPRVLSGVRGRGLLLGMMFPDNETGYK
                     VASGLFRRGVLVAGTQVSATTIRIEPALTIPEDLVEETIGRLDEALAELASA"
     gene            complement(6804..7466)
                     /locus_tag="VM841_05105"
     CDS             complement(6804..7466)
                     /locus_tag="VM841_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL89597.1"
                     /translation="MIYRVWWLLLVPLIRLYWRVRVTGAENVRRRGGMIIASNHQSGI
                     DPILVCASFWRHVYWLAKVELVITRKVAWFFKSVAVIPVDRNAPKEDSLQAAADCVRR
                     GRIFGIYPEGTRSPDEKVYRGHTGVARIAHLSGGAVIPTAVIGSRRSVPKGKLMARPT
                     KCVVRFGAPMTFAIKPGETEAAAYRRFTDDVMRAIAALAGTSYVPGVYSGDAARVEKL
                     AS"
     gene            7581..8021
                     /locus_tag="VM841_05110"
     CDS             7581..8021
                     /locus_tag="VM841_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL89598.1"
                     /translation="MCRMIPARISLVTIGAHDLPVLRTFYAALGWKESPISQEGFAAF
                     ETGGAVLALFPFDDLAADACIPPPVRRESFRGVTLAVNVAEKHEVDEAIATAREAGAQ
                     ILKEPTDAEWGGRTAYFADPEGNVWEVAWMPGSTFDERGALILP"
     gene            complement(8257..9630)
                     /locus_tag="VM841_05115"
     CDS             complement(8257..9630)
                     /locus_tag="VM841_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVL89599.1"
                     /translation="MLTRMLEFSRAMRDAGVPVAVSENLDALRALAYVPSEDREAFKA
                     ALAATMVKSEAHRKAFDTLFDLYWRTGRGTGAVEEQEQRDAPPGGSDPDQMQQEYMED
                     LFRTLLGGDASSIREAARMAVSRFGRVEAAQSGSPYFQYRVFRAVDLDSLLARLMQEV
                     ADGRELTPLEERMWRDEFEQRLREFRQEVEADVRRRLAEHRGAAQVAKHTVRPLPEDL
                     DFVRATRDEIVELRRAIRPLARRLATRLAMKRRAAHRGRLDVRATVRHSLSTGGVPFD
                     TRYRHKTPHRPELFMVCDISGSVAAFARFTLMFVHAFQAQFSRVRSFVFVDHLDEVTH
                     MFAGEDFLAAVDRMNQEADVVRFDGHSDYGNSLEAFWREYGSSIGPRTSVVILGDARN
                     NYRASASWVVKAIAQKARRVYWLNPEPINYWDTGDSIAADYARHASGMFEVRNLRQLE
                     EFIGRVI"
     gene            complement(9632..10504)
                     /locus_tag="VM841_05120"
     CDS             complement(9632..10504)
                     /locus_tag="VM841_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVL89600.1"
                     /translation="MRFETVQDVVDRLATAGYLADEAIATVVFLADRLEKPVLIEGPA
                     GTGKTELAKAVATILGDPLIRLQCYEGVDETKALYEWNYKKQLLRIQAGQDEGWDAIS
                     GDIFSDEFLLERPVLKAIRSEESVVLLVDEIDKMDFEGEALLLEVLSDFQVSIPELGT
                     LKARQIPFVVLTSNNSRELSEALKRRCLYLHLDYPDVDREKAIVLERVPEINETLADQ
                     VVRLVRSIRTLELKKSPSVAETIDWARTLLHLGITELDEAAARGTMGALLKYQTDVEK
                     VVAHLKIPPRPENN"
     gene            complement(10501..10962)
                     /locus_tag="VM841_05125"
     CDS             complement(10501..10962)
                     /locus_tag="VM841_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HVL89601.1"
                     /translation="MSQLRGEILAAEEAAWKELHDTFISLPGEQWSRHGLSGNWCPKD
                     LLAHVGCWAAEAARQLECIRAGSPSKGPDAEAFNAASYDTCKDLTVHEVIAMASAAHH
                     RLQEEIDAVAPVALSGKVVDMIHECSDRHYREHLAQLRIFIADIEAGEGTA"
     gene            complement(10959..12200)
                     /locus_tag="VM841_05130"
     CDS             complement(10959..12200)
                     /locus_tag="VM841_05130"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HVL89602.1"
                     /translation="MMIEIGKRAKAAARAMAAASTGVKDEALAGIARALKENADDILD
                     ANTADLERGEAGGVARALLDRLMLTPERIDSIAGAVRDVIALRDPVGEVIGGRKRPNG
                     LDITEVRVPLGVVGIIYEARPNVTVDAAALCIKSGNACILRGSRTAIESNTVLARLIR
                     GALADAGLPGDAVQLVEDTSRESVGELMKMRGTVDVLIPRGGAELIRSVVEGSSVPVI
                     ETGVGNCHVYVDAAADVSKAIPIVINAKTHRPSVCNAAESLLVHRAIADDVMPKIIEE
                     LSARGVEIRGDEATRSYSDAVVPATEEDYRTEFLDLTISAKIVGTLDEAIEHVNTHGT
                     LHTEAIVTEDYSAAQRFAAEVDAACVMINASTRFADGGEFGLGAEIGISTQKLHARGP
                     FALEALTTSKYVVRGSGQVRE"
     gene            complement(12264..13352)
                     /gene="proB"
                     /locus_tag="VM841_05135"
     CDS             complement(12264..13352)
                     /gene="proB"
                     /locus_tag="VM841_05135"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HVL89603.1"
                     /translation="MRALPDIRRLVVKVGSSSLTGADGLIDQRQISSLVTQITAARRN
                     RIDVVFVSSGAIAAGLPALGRKRRPTDIPTLQAAASVGQNILVHEYQRSFARRKVPVG
                     QVLLTQDDFVRRKGYLNARAAITRLLELGVVPIVNENDTVAVDEIRFGDNDRLAALVA
                     NLVHADLLVMLSDIDGLYTEDPRSHRATLIAEVTDPDHLSLVKAGKSRSGLGSGGMAS
                     KVEAARIATASGVHVVIASARNPRVIAEILSGRPVGTHFSPHRARGSSRKLWIRFAQT
                     TRGTIFVDAGAVNALVEGNRSLLPAGVVRATGSFALGDAVDVAGPDGAVFAKGLVNYA
                     SEEIAGVAGRSIRDGGREVIHRDSLVIL"
     gene            complement(13349..14644)
                     /gene="obgE"
                     /locus_tag="VM841_05140"
     CDS             complement(13349..14644)
                     /gene="obgE"
                     /locus_tag="VM841_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HVL89604.1"
                     /translation="MSRSGDFVDEAVVIVKGGDGGNGVASYRREKYRPKGGPDGGNGG
                     NGGNVVFRADPNIGTLSELARHPHQRAPRGQHGRGDDKHGANAPDLVLLVPEGTVVTD
                     ASTGEVVADLVRAPAEAIVARGGQGGRGNASLVSRARRAPGFAERGEVVEERTVRLEL
                     RVLADVGLVGLPNAGKSSLIARLSAARPKVAAYPFTTLTPHLGVAETEDGRFIVADIP
                     GLIEGAADGRGLGHAFLRHLERCLVLCYVIDVGSEDAEPAEAYRALRDELEAYDPAMA
                     ARASVVAANKSDLDGASERAEELRAALPAGTRMIKASALTGQGTEEIHEMLAAAVASA
                     REVEEPVADAHTMIKITPSSDKVHVVREDDGAFRVISDKAERLVARFDLDNDDAVAYL
                     QERFSTLGVEDALTKAGAVEGDEVRIGDAVFDFLPEHVS"
     gene            complement(14689..14940)
                     /gene="rpmA"
                     /locus_tag="VM841_05145"
     CDS             complement(14689..14940)
                     /gene="rpmA"
                     /locus_tag="VM841_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HVL89605.1"
                     /translation="MASKKGVGSSRNGRDSNAKRLGVKRYAGQNVNAGEILVRQRGTR
                     IHPGLGVGKGTDDTLFARVEGTVKFHRMGNRKVVSIVNA"
     gene            complement(15137..15715)
                     /gene="rplU"
                     /locus_tag="VM841_05150"
     CDS             complement(15137..15715)
                     /gene="rplU"
                     /locus_tag="VM841_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HVL89606.1"
                     /translation="MYAIIRAGGHQEKVSVGETITVDRLKQDPDSEIRFTPLLISVEE
                     GNAVSNRKELADKGAVVGRVIEHVRGDKIDVFHYKNKTGFRKHTGHRSMLTLVEITEI
                     GFGDKKETLDDAKKVSDAKAAEKAEASKALQEAKEKGKADRKAAAKKKSAAQKTTAAK
                     KTAAKKKAAPAKKAAASKKKAEAAPAESSSEE"
     gene            complement(15842..18040)
                     /locus_tag="VM841_05155"
     CDS             complement(15842..18040)
                     /locus_tag="VM841_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HVL89607.1"
                     /translation="MEEERKGTGAESAASAPTSLPPAAERPAAPPAERRAPPPVSGAA
                     SEGTGSGTGTGGGTPGADAPGDNGGDNGGDNGGDGDGTTASGAPRKRRRGRRGGRRHK
                     KKPAGAGGDGADGSDEPGGEDEDRTGPSGGSEQAAKPAGRPQQPRRQQQPRTRSAAGG
                     KDGDEPAKAGDDTGGDRPARSSARTGRTAATEAKAVKEPADGDGGAEPADTAGGNGEA
                     GTGQPRKRRRRGGRGRGGGGARKPAGQGGDEAASDSNDGGNGNGGGAATKAAQPAARS
                     SGGGQRQGSTRLAAQKQRREQRPRRPRRRLSEGEARALRGEAKTMLIHDHEDRTQIAV
                     LEAGVLIEHFVAHQKSRTIVGNVYLGKVQNVLPSMEAAFVDVGRGRNAVLYAGEVNYE
                     KEDLEGDGPPPRIEQILRPGQSVMVQVTKDALGTKGARLTAQISLAGRYLVLAPEQNV
                     SGISRRLAEDERARLRQIFRDIKPKEHGVIVRTAAEGAPRDALERDLNNLLKVWEGVQ
                     KNKKTAKAPALLHAEPELVMRVVRDIFSGDFERVVVDSRGMYDTIKGYLEEQGPDLIE
                     RIELHEGPLPMLEQYHVTEQIHKALERKVWLPSGGSIVIDRTEAMTVIDVNTGKFVGK
                     GGSLEDTVVKNNVEAAEEVARQLRLRDIGGIIVIDFIDMLLPANREQVLRTLKRALGR
                     DKTRSQVFDVSSLGLVEMTRKRVSEGLLDAFSEPCPNCDGRGIVITHDID"
     gene            complement(18070..19266)
                     /gene="rodA"
                     /locus_tag="VM841_05160"
     CDS             complement(18070..19266)
                     /gene="rodA"
                     /locus_tag="VM841_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HVL89608.1"
                     /translation="MPLRTDPSRRSRLFHPRVSRIGRQRIGQQGLPATAPLRHIDWVL
                     VLSALALTAIGILMIYSASFARLRDEGFTNEFLMNRQLLFAGLGVVACVLTAAIPYRR
                     YRTWAGALYGVGVLMLVAVLSGLGDASLGAQRWIDLGPLQLQPSEIMKIGVIVTLAAL
                     LSQARGTPGGLDVLRAILIVALPAALIFKQPDLGTLLVLVALLGGTLLVAGTKVRAML
                     TLLVLGIVAVWGVLQVGVLEDYQVARLTAFLDSSADPQVTGYNLNQAKIAVGSGGLTG
                     KGLFQGTQTNLDFVPEQHTDFIFTAIGEETGFVGSAVLLALFALFLWRGVRIAMLSKD
                     LFGTLIAAGVVSMITFQVFVNIGMTIGLSPITGIPLPFVSYGGSSLITNFIATGLLLN
                     VHMRRI"
BASE COUNT         2800 a         6838 c         6578 g         3139 t
ORIGIN      
        1 catcgtcgcc gtcctgatcg gcggcacgct cgccgggttc gccggcgcgc tgctcgccct
       61 gcccgccgcc gcgatcatca aggtgatcct gcagcgcgtc tatctgcacg agcgcctcgc
      121 ggtcgtgcag cgcgaggacg aggagcacgc cgcggcgaac gccggctcga agcccaccgg
      181 ctgacgcacg gcccctcgaa tccgtttcat ccgtttcacg aacattgtcg gtcttgggat
      241 gttctgcctt cgtagccaag cccggctcga taggcaacaa tcggtggcct cgggcgggag
      301 gtgcgtccga gccggcaagg gggatcgaac gcactgtcca ccacgacgac gcggcgtccg
      361 gcgcttcgcc tgatccgcac cccggcgcac ccgagcagga gcgagatgat gctccagcga
      421 gcgaacttcc ggctcgcaag acgacgcagg ctgaccctgc tgaccgtcgt cttcctcgcc
      481 acgctgtccg cgtgcatcgg gctcatcacg actcggcacg caactatgac cgggagcgcg
      541 atctcgttcc tcgccggcga cctgctcgtg ctggtctggg ccgggatcct ggtcgtgccg
      601 tcgttcgccg gctcggtcaa gccgcggccg gtgcgcgcgg gcgcgctggg ggcgctgctg
      661 ttcgggccgc tgctcatgcc cgtggtgttc gggccgggcg gctggaaagc gtggcaggtc
      721 gcggcgctcg tcgccgcgtc gattgcggcg ctcgtcccgc ctcgttcgct gctgcgctaa
      781 ctaggagcgc gccgccgcgg cggggccgtc ctcttcctcg aaatcgatga tcggcgcgtc
      841 gcgccagacc cgctcgatct cgtagtactc gcgctgctcc tgggcgaaca cgtgcacgac
      901 cacgtcgccg taatcgagca cgacccaggt gcgctcgcgc tcgccttcgc ggcggacggg
      961 cttgcgcggc gcgacggctt gctcgatctc gttgacgatc gtgcgcacct gccggtcggt
     1021 cgcgccggag cagatgacga agtagtcgca gatctcgatc agcgagccga cctccagaat
     1081 gcggatgtcg atcgccttct tcgccgaagc ggccctggca acgtcgatcg ccagggcctt
     1141 gggcggtgca agcgtggagt tcagcgcctc tcctcctctg tcgtcctagt ccctcggctc
     1201 gaaatcatga cccaccacga tggtcacgtc caccaccgtc tgggcgcggg tcccgatctc
     1261 gatctctcca acgcccaaaa gctcctgaat cttccgggcg gcgccgaggc ttcgctcgtc
     1321 tcgcgaatac accacgatct tggtcgtctc gaagtcgaac gagcgagcat tcttgttgag
     1381 cacgatccgg taccccttcg ggatcagctt cagcgccacc tcttcgccga tctgcgggat
     1441 gccgtttccg ttgagcacct cgatgcgggt tccaagatag gggttcttcg ggatggacgc
     1501 cgcgaaatac tggctcacca gattcccgac ctgatcctcg tcgatgcgaa gtgcttgctc
     1561 ggatccgccg gcggacaccg gctcgacggg aagggccgtg tagatccgat cggcgggaga
     1621 gccggcggtg aaggcgctga agaactcggc aaggtcgagc ggcgtgagcc cggtctcgat
     1681 gccttcgttc cactcgctgc cgatctcacg gaacttctcc gcgagcgctc cgacgcccag
     1741 ccggccccag gcggcgaaca tcgcttccca gacgtgctgc gcgcgggcga tcctggatag
     1801 ctcggtctcc ccgtcgcctt ggtagcgcag gtagccgcgg accgcctctc cgttcatctt
     1861 ctgcaggccc ggcgagaacg cgacaccgcc gcccggctcg atgagcgtgt cctccacggt
     1921 gatgtccagg ccgccggccg cgtccacgag cttgccgagc accgcatccg tcatcgcggc
     1981 gtctcggtcc agcacgatgc cgagcacgtt gtccacgacc agcgattgaa gccgcatgcc
     2041 gccgaactgg aatccgcgtc cggccagatc caggccgtgc cccggaatct cggtcagcgc
     2101 gttggtgggc atgaacagcg tgatcggatt cgtgccggcc gcgtcgagcg cgaacaccac
     2161 gagcatgtcg ctgcggcggg tcggatcgtc cgcctggcgg atcatgaaca ggatcgccgt
     2221 ctggccgtcc ttgtcaccgg ccagtacccc gctgccgccg cggccgcggt cgagccaggt
     2281 cgcgaccgcc cacgcggcgc cggcggcgag caccatcacc atcagcagcg cgaccgcacg
     2341 gctgcggcgc cgacggcggc ggttgcggcg cgtgcgtccg cccggctcga atccgagctc
     2401 gtcgagctcg ggcgaggatt gcatctcttc ttcggtgatc atcggtagag tccgctcttc
     2461 tcgatgtagg tcgcgacgcc ttccggcacg aggtagcgaa tcggccggcg ttccgcgacg
     2521 cgacggcgaa tgtcggtgga cgagatcgcg agcgcgggga tctccatcac gtccacgcgc
     2581 tcttccaatc cttccggcat ctgcttggcg agccggtcca ggtcgtagcc tggccgcgtg
     2641 caggcgatga accgcgcgcg gcgcagcacc tcctccggat ccttccagga caggatctcc
     2701 agcaccgcgt cggcgccggt gatgaacgat agatcgacgt ctccgagcgt ttccttcagc
     2761 agggcgagcg tctcgacggt ataggtcggg ccatcccggt cgatctcgac gcggctgacc
     2821 cgaaagcgcg gatgcgagga cgtggcgatc accgtcatca ggtatcggtc ctcggccggg
     2881 gccacgtcga tgccttcctt ctgccagggc cgaccggcgg gcacgaaaac gacctcatcg
     2941 agatcgaacg cgaccagggc ctcgtcggcc gcgacgagat gcccgctatg gatggggttg
     3001 aatgtgccgc ccatcacccc gatgcgccgg cgcgtcatcc gctcggcgag tctaacagcg
     3061 gctccggccg cccgaaggca tgacggaaga cattccgcaa cgaaccgctc gcccgggagt
     3121 tccgccagcc ggcaccggcc gggcgatcgt tagacgacga cgatctcgcc ggtcatccgc
     3181 ggatggagcg tgcagcgcac cttggtcgtg ccggaggtgg tgaaggtgaa ggtgacctcg
     3241 tcccccgcct tgagcggctt gtcgaagccc gagaacgtca cggtgtgcgg cacccgtccc
     3301 gtgttggtcc aggtgaccgt cccgcccgcc ttgagcagca cgcgcgccgg ctcgtaggtg
     3361 agcttgtcgg tcgagtccac cgtcacggcc gtcgccggct ccccggccgg cgtcggcgcg
     3421 gccgcgggat cgctgccgcc gacgcacgct cctgcgagga gcgacagggc cgcgacgatc
     3481 gcgaaacgcg tcaacggccg ctacgcgacc acgatggtgc cggccatgcc ctggccgttg
     3541 gccgtgccgt ggatcacgca gaagtacggg aacgatccgg ccgactcgaa ggtgaactcg
     3601 tactcctcgc ccgccttcga gcacttgttg atgtcggcct cgcactcggg gtgcgagtct
     3661 tcctcgaagg tcgtgaactt gacgttgtgc ggcgcactgc cggtgaagtc ccaggtgacg
     3721 gtttcgccga ccttgacctc gagctcggcg ggcttgaacg ccgagtccac gacgtcgacg
     3781 acggtctggg ccaccggttc gggcgtggga cccgacggaa gtccggtgtc cttggcgtcc
     3841 ccgcaggcga cgagcgcaag agcgaggatg gcggcgaaag cggccgggcg agcgagacga
     3901 gccatttcga gaatcctcct ggtgagcgtg aaaagcggca ggattctacg ccaacggcgt
     3961 gcccgttcgc ttgtgcggtt gcgacgagca gcctgcgggc ccggacggcc tgatagcctc
     4021 gatttcgtgc gagaggagct gctgcgtatc ggcggcgtcg cgcggcccgg aagcggccgc
     4081 tcgacgatcg tgtcgcccgc gacgggcgag cccgtcgcct cggtcgcggt cgcgtcggcc
     4141 tcggacgtgg acgaggccgt ccgcgccgcg caggcggcct tcgccgggcc gttcggcgcg
     4201 ctggccgagc acgagcggca ggcgatcctg cgccgcgcgt cggatctgct ggccgaacgg
     4261 gtcgaggacc tggcacgcac gatcacgctc gagaacggca agccgatccg cgatgctcgc
     4321 ggtgaggccg cccgcgcctg gcagaccatc ctgttcgcgg ccgaggaggc caagcgcatc
     4381 cacggcgagg tgctgccgat gggcgccgcg cgcggcggcg aaggccggat cgggttcacg
     4441 ctgcgcaagc cgatcggcgt ggtcgccgcg atcgccccct tcaactttcc gctgaacctg
     4501 gtctgccaca aggtcgggcc cgcgctggcg gcgggcaaca cggtcgtgct caagccgagc
     4561 gagaacacgc cgctgtcgtc gttcgcgctc gccgccgtct tcgaggaggc gggcctgccg
     4621 cccggcgcgc tcaacgtggt ctgcggcgac gccgaaaccg gcgaggcgct cgtggatcat
     4681 ccgggcgtgg cgatgatcac cttcaccgga tcggccgccg tcggaacgcg catccgctcg
     4741 cgcagcgggg tgcggcgcac tttgctcgag ctcggctcga acgcgccgat gatcgtcttc
     4801 gacgacgccg acatcggcgc tgcgttggag gccgcggtgc gcggctcgtt cacctactcc
     4861 gggcaggtgt gcatcagcat ccagcggctg ctggtgcagc gtccgctctt cgacccgttc
     4921 gtggtgcggc tggccgaggc ggcgtcggcg ctggtcgtgg gcgatccgct ggacgaggcc
     4981 acgcatatcg gcccgatgat cgacgaggac gccgcgaggc gggtcgagtc gtgggtggac
     5041 gcggccgtcg cgggcggcgc acgcgccgaa tgcggcggca cgcgtgacgg ccgcttctac
     5101 gccccgacgg tgctgaccgg cgtgaagccc gacatggacg tggtctgcaa cgagatcttc
     5161 ggcccggtcg tgtcggtgat gcccttcgac ggcctcgacg acgcgatcgc gatcgccaac
     5221 gacagcgtgt acgggctgca ggcgggcgtc ttcacgcggg acatcgaccg ggcgatgcgc
     5281 gtcgcgcagc ggctggacgt gggcgggctg tggatcaacg acgcgccgca gacacgcttc
     5341 gaccattatc cctacggcgg catgaagtcg tccggcctgg gccgagaagg cgtgcgctac
     5401 gccgtggaag agatgaccga gacgacgttc gtcgggatca agctcggcga cgcgcgctga
     5461 caggaggatc gtttggggtt cgatgacgcg gttcggcgat ccaaggagat gctcgacgtg
     5521 gtgctcgacg gcatcgactc gggcgaggcg cgcgaggcgc tcgccaagca gaccgtcatc
     5581 gacttcgacg aacacttcac gcccggcttg acccgctacc gcaagtccgt taccgaggcg
     5641 ggcgacttcg ccgccatcga atggaccggc gaaggctcga tcgtgtacga cgcgaagggc
     5701 cgcgaatgga tcgactgcct cggcggctac ggcatctact cgctcgggat ccgcaacccc
     5761 gaggtgatcg cggccgtgcg cgcccagatg gaccgcaacc cgatgccgac ccaagagctt
     5821 ctcgatccct tcaaggcgat gctgtgcgcg ctgctggccg agatcgcgcc cggcgacatg
     5881 cagaacgtgt tcgtcgcgaa ctccggcacc gagtcgatcg aaggcgcgat gaagatcgcg
     5941 cgcgtgcaca ccgggcggcc gggtttcgtg gtggccgaag cggccttcca cggcaagacg
     6001 ctcggcgcgc tgtcgctgat gggcaagccc gcgttccgcg aagccgtcga gccgctgctg
     6061 tcgccggtgt ggcgcgtgcc gttcggcgat gccgccgcgg tggacgcgat ccttcgcgat
     6121 cacgcgaccc aggtcgcggc cgtcgtgatg gagccgatcc aaggcgaggc gggcgcggtc
     6181 gttccgcccg aggacttctg gccgtcgctg cgcgcgatct gcgaccgcta cggcgtgctg
     6241 ctgatcgccg acgaggtcca gaccggcctc ggccgcaccg gaaagctatg gggcgtcgat
     6301 cactgggacg tcgcgcccga catcatcacc tcggccaagg cgctgggcgg cggcgtcatg
     6361 ccgatcggcg cggtcgcggg ccgggccgag atctggcggt cgctcgagcc gaacccgttc
     6421 cttcacacct cgacgttcgg cggcaacccg ctcgcgtgcg cggccgcgat cgccgcgatc
     6481 cacgtgatcc tgcgcgacca gctgtgggag cgggccgacc ggatcggcac ccgcttcgtc
     6541 gaggcgctca ccgacatggc ggaccgatac ccgcgcgtgc tgtcgggcgt gcgcggccgc
     6601 ggcctgctgc tgggcatgat gttccccgac aacgagaccg gctacaaggt cgcctcgggc
     6661 ctgttccgcc gcggcgtgct ggtcgccggc acgcaggttt cggccaccac gatccggatc
     6721 gagcccgcgc tgaccatccc cgaggatctg gtcgaagaga cgatcggccg actcgacgag
     6781 gcgctggccg agctggcgag cgcctagctg gcgagctttt cgacgcgggc ggcgtcgccc
     6841 gagtacacgc cgggaacata gctcgttccg gcgagcgccg cgatcgcgcg catgacgtcg
     6901 tcggtgaagc ggcggtaggc ggccgcctcg gtctcgccgg gcttgatcgc gaaggtcatc
     6961 ggcgcgccga agcgaaccac gcacttggtc ggacgcgcca tcagcttgcc cttcggaacg
     7021 gaacggcgcg atccgatcac ggccgtgggg atcaccgcgc cgccggacaa gtgcgcgatg
     7081 cgcgccacgc cggtgtggcc gcggtagacc ttctcgtcgg gcgagcgggt gccttccggg
     7141 tagatgccga agatgcggcc gcgacgcacg cagtcggcgg cggcctgcag cgagtcctcc
     7201 ttcggcgcgt tgcggtccac cgggatcacc gcgaccgact tgaagaacca ggcgaccttg
     7261 cgcgtgatca ccagctcgac cttggcgagc cagtagacgt ggcgccagaa cgaggcgcac
     7321 accaggatcg ggtcgatgcc ggactggtgg ttggaggcga tgatcatgcc gccgcgccgg
     7381 cgcacgttct ccgcccccgt gacccgaacc cgccagtaca gccggatcaa cggcaccagc
     7441 agcaaccacc agacgcgata gatcatcgat ccccctccct ctaccaggcg gtagattatc
     7501 cgtcggtggg tccgatttcc agggtgcggg gatcgggccc gcgaccaggg cgtttgcccg
     7561 tgtcgcggcg atcgggttcc atgtgccgca tgatccctgc gcgcatctcg ctcgtcacga
     7621 tcggcgccca cgacctgccg gtgctgcgca ccttctacgc ggcgctggga tggaaggaat
     7681 cgcccatctc gcaggagggc ttcgcggcgt tcgagaccgg cggcgccgtg ctggcgctgt
     7741 tcccgttcga cgatctggcc gccgacgcgt gcatcccgcc gccggtgcgc cgcgagtcgt
     7801 tccgcggcgt cacgctcgcc gtcaacgtcg ccgaaaagca cgaggtggac gaggccatcg
     7861 ccaccgcccg ggaagccggc gcgcaaatcc taaaggagcc caccgacgcc gaatggggcg
     7921 gccggaccgc ctacttcgcc gacccggaag gcaacgtctg ggaggtcgcc tggatgccgg
     7981 gctcgacctt cgacgagcgc ggcgcgctca tcctgcccta gcgaggggcc tgcaccccgg
     8041 ccgcagagtc cggaccgtag ccctcgacgg gatccttcac cccggtccag atcctccacc
     8101 ccgatccaga tcctccaccc ggatccagat cctccacccc gatcgactcg tcaactttct
     8161 tgacgagtgc cgtcgaagaa agtccgtggc cgagacggga cccgcgcgag gacgggcgcg
     8221 aaagaccggc gcgaggacgg gcgcgaaaga ccggcgctag atcacgcgtc cgatgaactc
     8281 ttcgagctgg cggaggtttc ggacctcgaa catgccgctg gcgtggcggg cgtaatcggc
     8341 ggcgatcgag tcgccggtgt cccagtagtt gatcggctcg gggttgagcc agtagacgcg
     8401 gcgtgccttc tgcgcgatcg ccttgacgac ccagctggcg ctggcgcgat agttgttgcg
     8461 cgcgtcgccg aggatcacca cgctggtccg cggtccgatg ctcgatccgt actcgcgcca
     8521 gaacgcctcg agcgagttgc cgtagtcgct gtgcccgtcg aaacgcacca cgtcggcctc
     8581 ttgattcatg cgatcaacgg ccgccaaaaa gtcctcgccg gcgaacatgt gcgtgacctc
     8641 gtcgagatga tcaacgaaca cgaacgaccg cacccggctg aactgggcct ggaatgcgtg
     8701 cacgaacatc agcgtgaagc gcgcgaacgc ggcgaccgag cccgagatgt cgcagaccat
     8761 gaacagctcc gggcgatgcg gcgttttgtg gcggtagcga gtgtcgaacg gcacgccccc
     8821 ggttgacagc gagtgccgca ccgtggcgcg cacgtcgagc cggccgcggt gggccgcgcg
     8881 gcgcttcatc gcgagccggg tcgcgagccg gcgcgcaagc ggccggatcg cgcggcgcag
     8941 ctcgacgatc tcatcccggg tcgcgcgcac gaagtcgagg tcctcgggca acggccgtac
     9001 cgtgtgcttg gcaacctgcg ccgccccgcg gtgctcggcg agacgccgcc gcacgtcggc
     9061 ttcgacctct tgccggaact cgcggagccg ctgctcgaac tcgtcgcgcc acatgcgctc
     9121 ctcgagcggc gtgagctcgc ggccgtcggc gacctcctgc atcaatcgcg cgagcagcga
     9181 atcgagatcc accgcccgga agacgcggta ctggaagtag ggcgagccgc tctgcgcggc
     9241 ctcgacgcgg ccgaagcgcg ataccgccat gcgcgcggcc tcgcggatcg acgaggcgtc
     9301 cccgcccagc agcgtccgga acaaatcctc catgtattcc tgctgcatct gatcgggatc
     9361 cgagccaccc ggcggcgcgt cgcgctgctc ttgctcttcg accgcgccgg tgccgcgacc
     9421 cgtgcgccag tacagatcga agagcgtgtc gaacgccttg cgatgcgcct cggacttgac
     9481 catcgtggcg gcgagcgcgg ccttgaacgc ctcgcggtcc tcgctcggca cgtaggccag
     9541 ggcacgcagc gcgtcgaggt tctcgctgac cgcgaccggc acgcccgcgt cgcgcatcgc
     9601 gcgcgagaac tcgagcatgc gcgtgagcac gtcagttgtt ctccggcctc ggcgggatct
     9661 tcaggtgcgc gaccaccttc tcgacgtcgg tctggtactt cagcagcgcc cccatggtgc
     9721 cgcgggccgc cgcctcgtcg agctcggtga tgcccaggtg cagcagcgtg cgcgcccagt
     9781 cgatggtctc ggcgacgctc ggtgacttct tcagctcgag cgtccggatc gagcggacca
     9841 gccgcacgac ctgatccgcg agcgtctcgt tgatctcggg cacgcgctct agcacgatcg
     9901 ccttctcgcg atcgacgtcc gggtagtcca ggtgcaggta caggcagcgg cgcttgagcg
     9961 cctcggacag ctcgcgcgag ttgttcgagg tgagcaccac gaacgggatc tgccgcgcct
    10021 tgagcgtgcc gagctcgggg atcgagacct gaaagtcgct gagcacctcc agcagcagcg
    10081 cctcgccttc gaagtccatc ttgtcgatct cgtccacgag caacacgacg gactcttcgc
    10141 tgcggatcgc cttgagcacg gggcgctcca gcaagaactc gtcgctgaag atgtcgccgc
    10201 tgatcgcgtc ccagccttcg tcctgcccgg cctggatgcg aagcagctgc ttcttgtagt
    10261 tccactcata gagcgccttg gtctcgtcca cgccctcgta gcactgcagc cggatcagcg
    10321 gatcgcccag gatcgtcgcg acggccttgg ccagctcggt cttgccggtg cccgccggcc
    10381 cttcgatcag cacgggcttt tcgagccggt cggcgaggaa gaccacggtc gcgatggcct
    10441 cgtcggccag gtagccggcc gtcgcgagcc ggtcgacgac gtcttgaacc gtttcgaacc
    10501 tcacgcggtg ccctcccctg cttcgatgtc tgcgataaag atgcggagct gcgcgagatg
    10561 ctcacggtag tggcggtccg agcactcgtg gatcatgtcc acgaccttgc cgctcagcgc
    10621 gacgggcgcg accgcgtcga tctcctcctg aaggcgatgg tgcgcggccg aggccatcgc
    10681 gatgacctcg tgcacggtca ggtccttgca ggtgtcgtag gacgcggcgt tgaacgcctc
    10741 ggcgtccggg cccttggacg gcgagccggc gcggatgcat tcgagctgcc gcgcggcctc
    10801 cgcggcccag cagcccacgt gcgccagcag atccttcggg caccaattcc cgctgaggcc
    10861 gtggcggctc cactgctcgc cgggaagcga gatgaacgtg tcgtgcagct ccttccacgc
    10921 ggcttcctcg gccgcgagga tctcgccgcg aagctggctc actcgcgcac ctggccgctg
    10981 ccgcgcacca cgtacttcga ggtcgtgagc gcctcgagcg cgaacggccc gcgcgcgtgc
    11041 agcttctggg tcgagatgcc gatctcggcg ccgagcccga actcgccgcc gtcggcgaag
    11101 cgagtcgaag cgttgatcat cacgcaggcg gcgtccacct cggccgcgaa ccgttgcgcg
    11161 gccgagtagt cctcggtgac gatcgcctcg gtatgcagcg tgccgtgggt gttcacgtgc
    11221 tcgatcgcct cgtcgagcgt gccgacgatc ttggccgaga tcgtcaggtc gaggaactcg
    11281 gtgcggtagt cctcctcggt cgcgggcacg accgcgtccg aatacgagcg cgtcgcctcg
    11341 tcgccgcgaa tctcgacgcc gcgcgcgctg agctcttcga tgatcttggg catgacgtcg
    11401 tctgcgatcg cgcgatgcac cagcagcgac tcggccgcgt tgcacacgct cggccggtgc
    11461 gtcttggcgt tgatcacgat cgggatcgcc ttcgagacgt cggcggcggc gtccacgtag
    11521 acgtgacagt tgccgacgcc ggtctcgatc accgggacgg acgatccttc gacaacgctg
    11581 cggatcaact cggcgccgcc gcgcgggatc agcacgtcca cggttccgcg catcttcatc
    11641 agctcgccga ccgactcgcg cgaggtgtcc tcgaccagct gcaccgcgtc cccgggcagg
    11701 cccgcgtcgg ccagcgcgcc gcggatcagc cgcgccagga ccgtgttgga ttcgatcgcg
    11761 gtgcgcgagc cgcgcaggat gcaggcgttg ccgctcttga tgcacagcgc agccgcgtcc
    11821 acggtcacgt tcggccgcgc ctcgtagatt atgccgacca cgccgagcgg cacgcgcacc
    11881 tcggtgatgt cgaggccgtt gggccgcttg cgcccgccga tcacctcgcc gaccggatcg
    11941 cgcagcgcga tcacgtcccg aaccgctccg gcgatcgaat cgatgcgctc gggcgtgagc
    12001 atcagccggt caagcagcgc gcgcgccacc ccgcccgctt cgccgcgttc cagatccgcc
    12061 gtgttggcgt ccaagatgtc gtcggcgttc tccttcaggg cccgcgcgat gcccgccagc
    12121 gcctcgtcct tgacgcccgt cgaggccgcc gccatcgccc gcgcagccgc cttcgcgcgc
    12181 ttgccgatct cgatcatcat gatccgatca gcctatcggt tccggcctat ccacgccccg
    12241 gcgaacggcc gcgatccgag cccctacagg atgacgagcg agtcgcggtg gatgacctcg
    12301 cggccgccgt cgcggatgct gcggccggcg acgcctgcga tctcttcgct ggcgtagttg
    12361 acgagccctt tggcgaagac cgcgccgtcc ggccccgcca cgtccacggc gtcgccgagc
    12421 gcgaaggatc ccgtggcgcg caccacgccc gcgggcagaa ggctccggtt cccctcgacc
    12481 aaggcgttga ccgcccccgc gtcgacgaag atcgtgccgc gcgtcgtctg cgcgaagcgg
    12541 atccacagct tgcgcgacga gccgcgcgcg cgatgcggcg agaagtgcgt gccgaccggc
    12601 ctgcccgaca ggatctcggc gatgacgcgc gggttccggg cgctcgcgat caccacgtgc
    12661 accccggacg ccgtcgcgat gcgtgcggcc tcgaccttgg acgccatgcc gcccgaaccc
    12721 agccccgacc gcgacttgcc ggccttcacc agcgacagat gatcggggtc ggtgacctcg
    12781 gcgatcagcg tcgcgcggtg gctgcgcgga tcctcggtgt agaggccgtc gatgtccgag
    12841 agcatcacca gcagatccgc gtgcaccagg ttcgcgacca gcgcggcgag ccggtcgttg
    12901 tcgccgaagc ggatctcgtc cacggcaacc gtgtcgttct cgttgacgat cggcaccacg
    12961 ccgagctcga gcagccgcgt gatcgcggcg cgcgcgttca ggtatccctt gcgccgcacg
    13021 aagtcgtcct gcgtgagcag cacctggccg acgggaacct tccgtcgcgc gaacgagcgc
    13081 tgatactcgt ggaccaagat gttctgcccg acggaggcgg ccgcctgcag cgtcgggatg
    13141 tcggtcgggc ggcgcttgcg tcccagcgcc ggcaatcccg ccgcgatcgc gcccgacgac
    13201 acgaacacca cgtcgatcct gttgcgccgc gccgccgtga tctgggtcac caacgacgag
    13261 atctgcctct ggtcgatcag cccgtccgcg ccggtcaggc tcgacgaacc gaccttaacg
    13321 accagacgcc ggatatcggg aagcgctctc acgacacgtg ctccggcagg aagtcgaaga
    13381 cggcgtcgcc gatgcgtacc tcgtcgcctt cgaccgcgcc ggccttggtg agcgcgtcct
    13441 cgacgccgag cgtcgagaag cgctcctgca agtatgcgac cgcgtcgtcg ttgtcgaggt
    13501 cgaagcgggc gacgagccgc tcggccttgt cggagatgac gcggaaggcc ccgtcgtcct
    13561 cgcgcacgac gtgaaccttg tccgacgagg gcgtgatctt gatcatggtg tgggcgtcgg
    13621 cgaccggctc ctcgacctca cgggccgacg cgaccgccgc agcgagcatc tcgtggatct
    13681 cctcggtgcc ctgcccggtc agcgccgacg ccttaatcat gcgcgtcccc gcgggcagcg
    13741 ccgcgcgaag ctcctcggca cgctcggatg cgccgtccag gtcgctcttg ttggcggcca
    13801 ccaccgaggc gcgcgcggcc atcgccggat cgtaggcctc gagctcgtcg cgcaacgcgc
    13861 gatacgcctc ggcgggttcg gcgtcctcgg agccgacgtc gatcacgtag cacaggacca
    13921 gacagcgctc gagatgacga aggaaggcgt ggccgaggcc gcggccgtcg gcggcacctt
    13981 cgatcaaacc gggaatgtcg gcaacgatga agcggccatc ctcggtctcg gcgacgccaa
    14041 ggtgcggcgt cagcgtcgtg aacggatacg ccgcgacctt cggccgcgcg gccgacagcc
    14101 gcgcgatcag cgacgacttg ccggcgttgg gaagacccac caggcccacg tcggcgagca
    14161 cgcgaagctc gagccgcacg gtgcgctcct cgacgacctc gccgcgctcg gcgaagccgg
    14221 gagcgcgacg ggcgcgcgac accagcgatg catttccccg cccgccttgc cctccgcgag
    14281 ccacgatcgc ctcggccggg gcccgcacca ggtcggcgac gacctcgccg gtggacgcgt
    14341 ccgtgaccac tgttccttcg ggaacgagca gcaccaggtc cggcgcgttc gcgccgtgct
    14401 tgtcgtcgcc gcggccgtgc tggccgcgcg gggcgcgctg gtgtggatgg cgggcaagct
    14461 cggacagggt tccgatgttc gggtcggcgc ggaagacgac gttgccgccg ttgccgccgt
    14521 tgccgccgtc ggggccgccc ttgggccggt acttctcgcg ccggtacgac gcaacgccgt
    14581 tgccgccgtc gccgcccttg acgatgacga cggcctcgtc gacgaaatcg ccggaacggc
    14641 tcatcaggag cgcatgctag cgggaaggac cctcgggagc ctacggggtt aggcgttgac
    14701 gatgctgacg accttgcggt tgcccatgcg gtggaacttg accgttccct cgacgcgggc
    14761 gaacagggtg tcgtcggtgc cctttccgac cccgagaccg gggtggatgc gcgtgccgcg
    14821 ctggcggacg aggatctcgc ccgcgttcac gttctggccg gcataacgct tgacgcccag
    14881 ccgcttcgcg ttggaatcgc gaccgttgcg gctggagccg acgcctttct tggatgccat
    14941 agcggcggaa gaataccagg gacccactcg gcaggccacc cgcgatttcc tcggaaggcg
    15001 gcgaaccgga cgaaaacggc gttctccggc gcgcacggcc cccattgggt cgagcccggc
    15061 tcgcccgaag ctgccgccct gcggcaatga cgaaacggcc ggggatcgcg tccccggccg
    15121 tcgtcgagtc gcgtcgttac tcttcggaag acgattcggc gggagccgcc tcggccttct
    15181 tcttcgaagc cgcggccttc ttggcgggcg cggccttctt cttggcggcc gtcttcttcg
    15241 cggcggtcgt cttctgagcc gcggacttct tcttggcggc ggccttgcgg tcggccttac
    15301 ccttctcctt ggcttcctgc agcgccttcg aagcctcggc cttttcggcg gccttcgcgt
    15361 cggacacctt cttggcgtcg tcgagcgtct ccttcttgtc accgaaaccg atctcggtga
    15421 tctcgaccag ggtgagcatc gagcggtggc cggtgtgctt ccggaaaccc gtcttgttct
    15481 tgtagtggaa cacgtcgatc ttgtcgccgc gcacgtgctc gatcacgcgt cccacgaccg
    15541 ctcccttgtc ggcgagctcc ttgcggttcg acacggcgtt gccttcctcg acgctgatga
    15601 gcagcggtgt gaagcggatc tcgctgtcgg gatcctgctt caagcggtcg acggtgatcg
    15661 tttcgccgac ggacaccttc tcctggtgcc cgcccgctcg aatgatcgcg tacatgtgtt
    15721 acttcccttc ctctcgacgg gtccggctgc cctgattgat cggggggcca ccttgcgccc
    15781 ccgaacccac gggcgattcc gttgggccgg ttcccagtcc gttcctcgtt atctcgaagt
    15841 ctcagtcgat gtcgtgggtg atgacgatac cgcgtccgtc acagttgggg cacggctcgc
    15901 tgaaagcgtc cagcagccct tctgatacgc gcttgcgggt catctcgacc aggcccaggc
    15961 tggacacgtc gaagacctgc gagcgggtct tgtcccggcc gagcgctcgc ttgagcgtgc
    16021 gaagcacctg ttcccggttg gccggcagga gcatgtcgat gaagtcgatg acgatgatcc
    16081 cgccgatgtc ccgcaggcga agctgccggg cgacctcttc ggccgcctcc acgttgttct
    16141 tcacgaccgt gtcctcgagc gagccgccct tgccgacgaa cttgccggtg ttgacgtcga
    16201 tgacggtcat cgcctcggtc cggtcgatca cgatcgagcc gcccgaaggc agccagacct
    16261 tgcgctcgag cgccttgtgg atctgctcgg tcacgtggta ctgctcgagc atcggcagcg
    16321 gtccttcgtg cagctcgatg cgctcgatca gatccgggcc ttgctcttcc aggtagccct
    16381 tgatcgtgtc gtacatgccg cgcgagtcca ccacgacccg ctcgaagtct ccactgaaga
    16441 tgtcgcggac cacgcgcatg accagctcgg gctcggcgtg cagcagcgcg ggcgccttgg
    16501 cggtcttttt gttcttctgg accccttccc agaccttgag caggttgttg aggtcccgct
    16561 cgagcgcgtc gcgtggcgcg ccctcggccg ccgtgcggac gatcacgccg tgctctttcg
    16621 gcttgatatc gcggaagatc tgccgcaggc gtgcgcgctc gtcctcggcc agacggcggg
    16681 agatgcccga cacgttctgc tcgggcgcga gcaccaaata gcggccggcg agcgagatct
    16741 gcgcggtcag gcgcgcgccc ttggtgccga gcgcgtcctt ggtcacctgg accatcaccg
    16801 actggccggg cctgaggatc tgctcgatgc gcggcggcgg cccgtcgccc tcgagatcct
    16861 ccttctcgta gttgacctcg cccgcgtaga gcacggcgtt gcggccgcga cccacgtcca
    16921 cgaaggccgc ttccatcgac gggagcacgt tctgcacctt gccgaggtag acgttgccca
    16981 cgatcgtgcg ggacttctgg tgcgcgacga aatgctcgat cagcacaccg gcctcgagca
    17041 ccgcgatctg cgtgcggtcc tcgtggtcgt ggatgagcat cgtcttggcc tcgccgcgca
    17101 gcgcgcgcgc ctcgccttcc gacagccgtc gtcgcggccg gcggggacgc tgctcgcgcc
    17161 gctgcttctg cgccgccagc cgggtcgagc cctgccgctg tccgccgccc gacgagcgcg
    17221 cggccggctg agcggccttg gttgcggccc cgccgccgtt gccgttcccg ccgtcgttcg
    17281 aatccgacgc ggcctcgtcg ccgccttggc cggcgggctt gcgagcgcct ccgcctccgc
    17341 ggccgcggcc gccgcgacgc cgccgcttgc ggggctggcc cgttccggcc tcgccgttgc
    17401 ctccggcggt gtcggccggc tcggcgccgc cgtcgccgtc ggcaggctcc ttgacggcct
    17461 tggcctcggt cgcggccgtt cggcccgtcc tggccgaaga cctggccggc cggtcgccgc
    17521 ccgtgtcgtc cccggccttg gcaggctcgt cgccgtcctt gccgcccgcg gccgagcggg
    17581 tgcgcggctg ctgctgccgg cgcggctgct gcgggcgtcc cgccggcttg gcggcttgct
    17641 cgctgccgcc ggacggaccg gtcctgtcct catcctcgcc gccgggctcg tccgagccgt
    17701 cggctccgtc gccgcccgcg ccggccggct tcttcttgtg ccggcggccg ccccggcggc
    17761 cccggcgacg cttgcgcggc gcacccgagg cggtcgtccc gtccccgtcg ccgccgttgt
    17821 cgccgccgtt gtcgccgccg ttgtcgcccg gcgcgtcggc gccgggcgtg cccccgccgg
    17881 tgcccgtccc cgagccggta ccctccgagg ccgcgcccga cacgggcggc ggcgcgcgac
    17941 gctcggcagg cggggccgca ggacgctccg ccgctggcgg cagagacgtc ggcgcggagg
    18001 cggcgctctc ggcgccggtc cccttgcgtt cttcttccac gtggtctcct tcgttgcgtt
    18061 gcggtcggat cagatccgcc gcatatggac gttgagcaga agcccggtcg cgatgaagtt
    18121 ggtgatcagc gacgagccgc cgtacgagac gaacggcagc gggattccgg tgatcggcga
    18181 caggccgatc gtcattccga tgttgacgaa gacttggaac gtgatcatcg acacgacgcc
    18241 cgcggcgatg agcgttccga acagatcctt cgacagcatg gcgatgcgca cgccgcgcca
    18301 cagaaacagc gcgaacagcg cgagcagcac cgcggaaccg acgaagcctg tctcttcgcc
    18361 gatcgcggtg aagatgaagt cggtgtgctg ctcgggcacg aagtccaggt tcgtctgcgt
    18421 gccttggaac aggcctttgc cggtcagacc gcccgacccc acggcgatct tggcctgatt
    18481 gaggttgtag cccgtgacct gcgggtcggc cgacgaatcc aggaacgccg tgagccgggc
    18541 gacctgatag tcctcgagca caccgacctg cagcacaccc cagacggcga cgatgccgag
    18601 caccagcaag gtcagcatcg cgcgcacctt cgtgcccgcc accagcagcg tgccgccgag
    18661 caacgcgacc agcaccagca gcgtgccgag gtccggctgc ttgaagatca gcgcggccgg
    18721 cagcgccacg atcaagatgg cccgcagcac gtcgagcccg cccggcgtgc cccgcgcctg
    18781 cgacagcagg gcggcgagcg tgacgatcac gccgatcttc atgatctcgg acggctgaag
    18841 ctgcagcggc ccgaggtcga tccatcgctg tgcgccgagc gaggcatctc ccagacccga
    18901 caggaccgcg accagcatca ggacgccgac gccgtagagc gctcccgccc aggtccggta
    18961 gcggcgatac gggatcgccg ccgtcaacac gcacgccacg acgcccagcc cggcgaacag
    19021 gagctgccgg ttcatcagga actcgttggt gaagccttcg tcgcgaagcc gtgcgaacga
    19081 cgccgaatag atcatcagga tgccgatcgc ggtcaacgcg agcgcactca gcacgagcac
    19141 ccagtcgatg tggcgcagcg gcgcggtcgc gggcagcccc tgctgcccga tgcgctggcg
    19201 gccgatgcgc gacacgcgcg gatggaacag ccgcgagcgg cgggagggat cggtgcgcag
    19261 cggcatcagt cgctcctccc ggttccgtac acgatcggcg tcagcggcaa cccaaagagc
    19321 tggtccatga tccgccgcac gatcgggccc gagac
//
LOCUS       DATLFO010000137        11213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_45644, whole genome shotgun sequence.
ACCESSION   DATLFO010000137 DATLFO010000000
VERSION     DATLFO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45644"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..577)
                     /locus_tag="VM841_05165"
     CDS             complement(74..577)
                     /locus_tag="VM841_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVL89609.1"
                     /translation="MSELETRAERLTPREWRVVALVIEGVPTSQACERLGIASGTFES
                     HKQAIRRKLGIPRGERLEKHLQIFAGNIPAPAERPERIAAVSSEVPADPSERRIRLLY
                     RMTIDELTEVAGSAELRATLLGQTVKRMQSDDHADAEREIADLREAATEIRTLAARLL
                     AAVRSRR"
     gene            complement(626..4300)
                     /gene="dnaE"
                     /locus_tag="VM841_05170"
     CDS             complement(626..4300)
                     /gene="dnaE"
                     /locus_tag="VM841_05170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVL89610.1"
                     /translation="MTDSFVHLHVHTEYSILDGAARIKELIGETAAMGMPAIAMTDHG
                     NMYAALDFYRECRKQGVKPIIGAELYVATRSRFEKSSREKDGSRHLTVLATSDQGYRN
                     LMKIVSAGHLDGFFYRPRVDKEILSAHSEGIVVLTGCLASEVNEHLKSGDVEAATSSA
                     GEYAEIFGKDNVYVELQDHGIADQHKVFPQLLEVARRTGLPTVATNDLHYTRKTDAAA
                     HDVLLCIQTNAKVGDANRFKFDGEEFYLKSPEAMRELFRDHRDACDATLEIAERCQIE
                     IEFGTLHLPPFTPPDGESQEDYLRRLTYEGAEQRYGSPLPLNHRERIEHELNVIISMG
                     FAGYFLIVADLIAFARSRGIRVGPGRGSAAGCVVSYCLRITDLDPMRYGLIFERFLNP
                     ERRQMPDIDMDFDERRRGEVIRYATEKYGEDHVAQIITFGTIKGKQAIRDAARVLDLP
                     YTVGDRLAKMYPPAILGKEAPLAACFDKSIAWPDKEVARNDAFQNASDLRKAYEEEDM
                     SRQVIDIARTLEGLRRQVSVHAAGVVIADRPIVEYVPLKRADEANGGGIVTQYEMGGV
                     ESLGLLKMDFLGLRNLTVLTDTIANLTRTKGIDLDIDNVPLEDRETFRMLTAGYTIGV
                     FQLESPGMRELVKRLRPDSFGDVMALVALYRPGPLGQGMHIEYAERKHGNRKVTYLHP
                     DLEPILNETFGIILYQEQALRIAVDMAGFSMAEADTLRKAIGKKIADVMRAQKEKFIA
                     GCVAKGYTEELGTQLWTLIDEFSGYGFNKSHSAGYALIAYQTAYLKATHPVEYFAALL
                     TSVRGNQDRSALYLAECRALGIRVHPPDVNLSDSDYTPTKNGEIRFGLSAVRNIGEHV
                     VALIVKEREERGLFKSFTDFCHRVDATVLNKRVVEALVKAGAFDSLWEEGAAKRAAFL
                     EWGTDKRTGLPTLGLSEPAAKMLEAAAEDRRNEAAGQSSLFGAEERQSMTTVSEPVSS
                     GAIIDKRALLTAEKEMLGLYVSDHPLLAVEASLRDLCDTQIADVETLEEGRTVTIGGI
                     ISRSQKKFTKKGEAMGVLTLEDLAGAIEVVVFPSTYLSLPAEVIAQDAIVVVKGKVDV
                     REEPPKIVAQEITKPVLAVSEAPATRNEPGDPKPLSGGAVADTTRRAANGRRRAAART
                     GDDTLVLRLSAERAGDPAFVAELRGVLSSHPGPTPVHLHLTSGEGTTKVLRLADDYQV
                     ERRQGLYAELKAILGSGAVE"
     gene            4613..5776
                     /locus_tag="VM841_05175"
     CDS             4613..5776
                     /locus_tag="VM841_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVL89611.1"
                     /translation="MDELILDLPAASPPAPPGSRLSYSSLSTYETCGLQYKLRYLDRL
                     PVRPSPILSFGNSLHEALRRWYNQPVPVPPPVEELLAHLDDVWDESQFPSAAASRTYK
                     DHGRQVLTAFHEANAPSFRIPVALEQRFEIDVEGVKVSGVIDRMDRHPDGTYEIIDYK
                     TNRRLPPLSRVEKDLQLSIYYLAAWEVWGIRPDKLTLYFLLPGQPLSGVRTPEDAAAT
                     RERILRVAEQIRGGDFAPRDNPLCNWCDFQARCPLYSHMLAPPPEDGETGIQPLVDEW
                     IGRKKRMLADWRRLEEIAPLIHEYCERNGLQRVFGKGGSITRFERAGDTFDPALVRDA
                     LPPEMLEQVIRVDDAAVGELLAGDLPDEIRARIEEARASEATWSLRLREDRKR"
     gene            complement(5773..6354)
                     /locus_tag="VM841_05180"
     CDS             complement(5773..6354)
                     /locus_tag="VM841_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89612.1"
                     /translation="MKGIRAAASVLMLASVLAAAGPGHAGVQIVQSERAWAQWSGGAG
                     CLQSFVHVLAERSAVLDPPGAPPRVSVAWVSVVKADACSGAVFESSHGRAFVRGLRIG
                     PQSASLRATVDLQDAVSGTTRPVRVEVDWTAGSVEPVTLTDHDRGVVRITASSADAAA
                     TGSVVRDTGPAATGEAAAGGLLTFRRIAAGRDG"
     gene            complement(6427..7332)
                     /locus_tag="VM841_05185"
     CDS             complement(6427..7332)
                     /locus_tag="VM841_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVL89613.1"
                     /translation="MSGISFEVAAEEAGHRLDVVLARRTGLSRSAAAALSVTVDDRPA
                     SKSLKLERGQRVEADLPDAAPAEPQPEDIDVPVVYEDDALVVISKPAGLVVHPAPGHP
                     GGTLVNALLARAGRPAGGEAWRPGIVHRLDAGTSGLMIVAKDEHVHATLVGMLASRTV
                     KRTYTALVEGEPESPTATIDAPIGRSPRHRKKMAVVAGGREAITHYQVMERLGEAALL
                     EVRIETGRTHQVRVHLAEIGHAVVGDQVYGRGRKLAARLGLERPFLHAARLELAHPLS
                     GAPLAFEDPLPEDLARALETARTGP"
     gene            complement(7329..7877)
                     /gene="lspA"
                     /locus_tag="VM841_05190"
     CDS             complement(7329..7877)
                     /gene="lspA"
                     /locus_tag="VM841_05190"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVL89614.1"
                     /translation="MQTAGRATQISWRALLALSVTAAAGLIADQITKALALAKLPEGG
                     TVPFIEGVWHWTLQRNPGAAFSLFTTVPWLFTIIAFVISAGIIWYARRVHDPGLGLAL
                     GFVLGGALGNLSDRIVRPPAPFRGHVIDFIDFRIWPTFNIADVCVVTGAALLFLHGVR
                     AERAEKRAAEAAARGGDGGATS"
     gene            complement(7847..8614)
                     /locus_tag="VM841_05195"
     CDS             complement(7847..8614)
                     /locus_tag="VM841_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HVL89615.1"
                     /translation="MARPTKKSSATAAAKKTEAARAAAKPSAKKTSAKAPIKSSSKKT
                     IPAKKSAPAKKTAPAKKAAAKKEAPAKKAPPAKKEAPAAKAVPAKKETPAAKAPASET
                     KLAPAKKIVAKTPAAAAAPAAPKPKSKPPKFSPKELDAIRQDLLDHRATYEKEFEDLE
                     SGSLNVAQSEMSGEVSYDEEFADAGTFTFERERDLSLSNNIRDLMDKVDTAMRKLDEG
                     SYGVCERCGNAIDKARLKALPYSVLCITCKQQEERLR"
     gene            8898..>11213
                     /gene="ileS"
                     /locus_tag="VM841_05200"
     CDS             8898..>11213
                     /gene="ileS"
                     /locus_tag="VM841_05200"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HVL89616.1"
                     /translation="MSVFRPVDSRIRWPEMEQRVAERWRERDVFARSQHLHEGGRRWN
                     IYEGPPTANGKPGVHHVLARVFKDLFPRYRTMRGDFVPRKGGWDCHGLPVELEVEKEL
                     GLSSKRDIEAFGIEEFNRRCRESVLRYVGEWRRMTERIAYWTDMDDPYYTMSNDYVES
                     VWWSLKKIYDDGLLFEDYKVTPYCARCGTPLSDHEVAQGYQTVTDPSVFVRFPLTDET
                     GTSLLVWTTTPWTLLSNAAAAIHPEVPYVAVQYESERLIVAEPLVHRIFGDDAEVVEH
                     FEPGSLEGRSYTPPFDFAAPDKRAWYVVAAEYVTTEDGSGIVHTAPAYGAEDMETGRA
                     NDLPVIHMVDAEGKFVDAVTPWAGQFVKDADPSIIENLRERGILFREETIEHTYPFCW
                     RCKTPLLYYARNSWYIRTTARKDDLLAQNETIAWHPDHIKHGRFGDWLENNIDWALSR
                     DRYWGTPLPVWRCEQKHTVCIGSFAELGERSGRDLSGFDPHRPYVDDITFACVECGGE
                     MRRVTSVIDTWYDSGSMPFAQWHYPFDNEQTFEAAFPAQFIAEGIDQTRGWFYSLLAS
                     STLLFGQSSYERVICHGHIVDKDGRKMSKSLGNAIDPWTILDGQGADALRWYLLTIGS
                     PWTSRRLSLDAIDEVLRGFMRMWWNVYSFYVLYANTDGIDPASLDAPPPAERGELDRW
                     ILAELHDTVRGVTDALEAFDAADAGRRITAFVDELSNWYVRRSRRRFWAGAESADKAA
                     AHATLYECLVTMAKLVAPFCPFTADEMWDNLV"
BASE COUNT         1679 a         3909 c         3741 g         1884 t
ORIGIN      
        1 cggtgagcgc gctcgtcgtc taagtcaggc cggcacggct catcctgggt cccttccctg
       61 aagggcggcg gagctatcgc cggctgcgga cggccgcgag gagccgtgcc gccaatgtgc
      121 ggatctccgt tgccgcctcc cgcaggtcgg cgatctcccg ttcggcgtcg gcgtggtcgt
      181 cgctttgcat gcgcttgacg gtctggccga gcagcgtcgc gcggagctcg gcggatccgg
      241 cgacctcggt gagctcgtcg atggtcatcc gatacagcag ccggatgcgt cgttcgctcg
      301 ggtcggcggg gacctcgctg ctgaccgccg cgatccgctc cgggcgctcg gcgggcgcgg
      361 ggatgtttcc ggcgaagatt tggagatgct tctcgaggcg ttccccgcga gggatgccga
      421 gctttcgccg gatggcctgc ttgtggcttt cgaacgttcc cgaagcgatg cccagccgtt
      481 cgcatgcctg cgacgtgggg actccttcga tgacgagtgc gacgacacgc cattcacggg
      541 gcgtgagccg ctcggcacgg gtttcgagct cactcatggc ctgatcactt tacgcgagag
      601 ttaagaagaa acggtggagg ctcgcttact cgaccgcccc cgacccgagg atcgccttca
      661 actccgcgta gaggccctgg cgccgctcca cctgatagtc gtcggccagg cgcagaacct
      721 tcgtggtgcc ctcaccgctc gtcagatgca gatgcacggg cgtcgggccc ggatgcgagc
      781 tcagcacgcc gcgcagctcg gcgacgaagg ccgggtcgcc ggcgcgctcg gcggacaggc
      841 gcagtacgag cgtgtcgtcg ccggtgcggg cggcggcacg ccgccgcccg ttcgccgccc
      901 gccgggtcgt gtccgcgacc gcgccgcccg acagcggttt ggggtcgccc ggctcgttgc
      961 gggtggcggg cgcttccgag acggccagca cgggcttggt gatctcctgc gcgacgatct
     1021 tgggcggctc ctcgcgcacg tccacctttc ccttgaccac cacgatcgcg tcctgcgcga
     1081 tcacctcggc cggcagcgac aggtaggtgg acgggaagac cacgacctcg atcgcgcccg
     1141 cgagatcctc gagcgtgagc acgcccatcg cctcgccctt cttggtgaac tttttctgcg
     1201 accgcgagat gatcccgccg atggtgacgg tccgtccctc ctccagcgtc tcgacgtcgg
     1261 cgatctgcgt atcgcacagg tcgcgcagcg aggcctcgac tgcgagcagc ggatgatcgg
     1321 acacgtacaa accgagcatc tccttctcgg ccgtgagcag cgcgcgcttg tcgatgatcg
     1381 cgcccgacga gaccggctcg ctcacggtcg tcatcgactg ccgctcctcc gcgccgaaca
     1441 gcgacgactg gcccgcggcc tcgttgcgac gatcctccgc ggccgcttcg agcatcttgg
     1501 cggccggctc ggacaggccg agcgtgggca agccggtgcg cttgtcggtt ccccactcca
     1561 agaacgccgc gcgtttggcg gcgccctcct cccatagcga gtcgaacgcg ccggccttga
     1621 ccagcgcctc gacgacgcgt ttgttgagca ccgtcgcgtc cacgcgatgg cagaagtcgg
     1681 tgaacgactt gaacagcccg cgctcctcgc gttccttgac gatcagcgcg acgacgtgct
     1741 cgccgatgtt gcgcacggcc gacaggccga agcgaatctc gccgttcttg gtcggcgtgt
     1801 agtccgagtc cgacagattc acgtcgggcg gatgcacgcg gatgccgagc gcgcggcact
     1861 cggccaagta aagcgcgctg cgatcttggt tcccgcgcac gctggtcagc agcgcggcga
     1921 agtactcgac cggatgcgtc gccttcaggt aagcggtctg gtacgcgatc agcgcgtagc
     1981 ccgcgctgtg gctcttgttg aacccgtagc ccgagaactc gtcgatcaag gtccacagct
     2041 gcgtcccgag ctcttcggtg tatcccttgg cgacgcaacc ggcgatgaat ttctccttct
     2101 gcgcgcgcat cacgtcggcg atcttcttgc cgatcgcctt gcgcaacgtg tcggcctcgg
     2161 ccatcgagaa cccggccatg tccaccgcga tccgaagcgc ctgctcctgg tacaggatga
     2221 tgccgaacgt ctcgttgagg atcggctcca gatccggatg caggtaggtg accttgcggt
     2281 tgccgtgctt tcgttccgcg tactcgatgt gcatgccctg gccgagcgga cccgggcgat
     2341 acagcgcgac gagcgccatc acgtctccga agctgtcggg ccgcagccgc ttgaccagct
     2401 cgcgcatgcc gggcgattcg agctggaaca cgccgatggt gtagccggcc gtgagcatcc
     2461 ggaaggtctc gcgatcctcg agcggcacgt tgtcgatgtc gaggtcgatg cccttggtgc
     2521 gcgtgaggtt cgcgatcgta tcggtgagca cggtcaggtt gcgcaggccg agaaagtcca
     2581 tcttcagcag gccgagcgat tcgacgccgc ccatctcgta ctgggtgacg atgccgccgc
     2641 cgttggcctc gtccgcgcgc ttgagcggca cgtactcgac gatcgggcgg tcggcgatca
     2701 cgacaccggc cgcgtgcacg ctgacctggc ggcgaaggcc ttccagggtg cgggcgatgt
     2761 cgatgacttg ccgcgacatg tcctcttcct cgtaggcctt tcgcaggtcg gacgcgttct
     2821 ggaaggcgtc gttgcgggcg acctccttgt ccggccacgc gatggacttg tcgaagcacg
     2881 cggccagcgg cgcttccttg ccgaggatcg cgggcgggta catcttggcg agccggtccc
     2941 ccaccgtgta cggcaggtcg agcacacgcg ccgcgtcacg gatcgcctgc ttgcccttga
     3001 tggtaccgaa cgtgatgatc tgcgcgacgt gatcctcgcc gtacttctcg gtcgcgtagc
     3061 ggatcacctc gccgcggcgg cgctcgtcga agtccatgtc gatatcgggc atctgccggc
     3121 gctcgggatt gaggaagcgc tcgaagatca gcccgtagcg catcgggtcg aggtcggtga
     3181 tgcgcaggca gtacgacacg acgcagccgg ccgcgctgcc acggcccggg ccgacgcgga
     3241 tgccgcgtga acgcgcgaag gcgatcaggt cggcgacgat caggaagtag cccgcgaagc
     3301 ccatcgagat gatcacgttc agctcgtgct cgatgcgctc gcgatggttc aagggcagcg
     3361 gcgagccgta gcgctgctcg gcgccctcat aggtcagccg gcgcaggtag tcctcttgcg
     3421 actcgccgtc cggcggcgtg aacggcggca ggtgcagcgt gccgaactcg atctcgatct
     3481 ggcaccgctc ggcgatctcg agcgtcgcgt cgcacgcgtc acggtggtcg cggaacagct
     3541 cgcgcatcgc ttccgggctc ttgaggtaga actcctcgcc gtcgaacttg aatcggttgg
     3601 cgtccccgac cttggcgttg gtctggatgc acagcagcac gtcgtgggcg gctgcgtcgg
     3661 tcttgcgggt gtagtgaagg tcgttggtcg ccaccgtggg caggccggtt cggcgcgcga
     3721 cctcgagcag ctgcgggaag accttgtgct ggtcggcgat gccgtggtcc tgcagctcga
     3781 cgtagacgtt gtccttgccg aagatctccg cgtactcgcc ggcgctgctc gtggccgcct
     3841 cgacgtcgcc gctcttgagg tgctcgttga cctcgctcgc caggcagccg gtcaggacca
     3901 cgatgccttc gctgtgcgcg ctcaggatct ctttgtccac gcgcggccgg tagaagaacc
     3961 cgtcaaggtg gcccgcggac acgatcttca tcaggttgcg gtagccctga tcggaggtcg
     4021 ccagcacggt caggtgccgc gagccgtcct tctcgcggga ggacttctcg aagcgcgacc
     4081 gcgtcgccac gtacaactcg gcgccgatga tcggcttgac gccctgcttg cggcactccc
     4141 ggtagaaatc gagcgccgcg tacatgttgc cgtgatcggt catcgcgatc gccggcatac
     4201 ccatggcggc cgtctcgccg atcagctctt tgatccgcgc cgcgccgtcc aggatcgagt
     4261 actcggtatg gacgtgaaga tggacgaagg aatccgtcat ctcgctctca ccagctcacc
     4321 agctcaccac gaagcttcac ccacccccgc ggacccgctc gcttccgggc cccgaaaacc
     4381 gttgacggcg gtcccggccg cgctggccga tgccgccgtt cgcgcatgca tacgtacggt
     4441 aggggaaggc acgtccgcgg aattacaggt gtgtcattcc gcggagacca gcctacccgg
     4501 cgggcggcgg cgcttcaaga gcgggaagat ccccggggag gcccgaacgc aagaatcatt
     4561 cgaaatcaca ggcctgtaat ccgtccccgg ggagtgctag tctcggacgc tgatggacga
     4621 gctcatcctg gacctgcccg cggcctctcc cccggcaccg cccggctccc ggctgtccta
     4681 ttcgagcctg tccacctacg agacctgcgg gctgcaatac aagctccgct acctggaccg
     4741 gctccccgtc cggccctcgc cgatcctgtc gttcggcaac tcgctccacg aggcgctgcg
     4801 gcgctggtac aaccagccgg tcccggtccc gccgccggtc gaggagctgc tcgcccacct
     4861 cgacgacgtg tgggacgagt cccagttccc gagcgcggcc gccagccgta cctataagga
     4921 tcacggccgg caggtcctca ccgccttcca cgaggccaac gcgccgtcct tccgaatccc
     4981 ggtcgcgctc gagcagcggt tcgagatcga cgtcgaaggc gtgaaggtct cgggagtcat
     5041 cgaccggatg gaccgccacc cggacggcac ctacgagatc attgactaca agaccaaccg
     5101 gcgcctgccg ccgctgtccc gggtcgagaa ggacttgcag ctttccatct actatctggc
     5161 cgcctgggag gtctggggaa tccgcccgga caagctcacc ttgtatttcc tcctgcccgg
     5221 ccagccgctg tccggcgttc gcacgcccga ggacgcggcc gcgacccgcg agcgcatcct
     5281 gcgggtggcc gagcagatcc gcggcggtga cttcgccccg cgcgacaacc cgctgtgcaa
     5341 ttggtgcgac tttcaggccc gatgcccgct gtactcgcac atgctcgcgc caccccccga
     5401 ggacggcgag accggcattc agccgctggt ggacgagtgg atcggacgca agaagcgcat
     5461 gctggccgac tggcgccgcc tcgaggagat cgcgccgctc atccacgagt actgcgagag
     5521 aaacggcttg cagcgtgtct tcggcaaagg cggctcgatc acccggttcg agcgtgcggg
     5581 cgatacgttc gatccggcgc tggtccgcga cgcgctcccg cccgagatgc tcgagcaagt
     5641 gatccgcgtg gacgacgccg cggtcggcga gctgctcgcc ggcgacctgc ccgacgagat
     5701 ccgcgcccgc atcgaggagg cgcgcgcctc cgaggccacc tggtcgcttc gcctgcgcga
     5761 agaccggaag cgttaaccgt cccgcccggc ggcgatgcgc cggaacgtca acaggccccc
     5821 ggcggctgcc tcgccggtcg cagccggtcc cgtgtcgcgc acgacgctgc cggtggcggc
     5881 ggcgtcggcc gaggatgcgg tgatgcgcac cacgccgcgg tcgtgatcgg tgagcgtgac
     5941 cggctcgacg gacccggccg tccagtcgac ctcgacgcgc acgggccggg tggtcccgct
     6001 gacggcgtcc tgcagatcca cggtcgcgcg aagcgacgcg ctttggggac ctatgcgcag
     6061 gccgcgcacg aatgcgcggc cgtgcgagga ctcaaacacc gcgccgctgc aggcatcggc
     6121 tttgacgacc gagacccatg ccaccgacac gcgcggcggc gcaccgggcg gatcgagcac
     6181 ggccgatcgc tcggccagca cgtgcacgaa cgactgcagg catccggcgc cacccgacca
     6241 ctgcgcccag gctcgctcgc tctgcacgat ctggactccg gcgtggccgg gccccgcagc
     6301 cgccagaacc gacgccagca tgagcacgct cgccgcggct cgaattcctt tcacgtgcct
     6361 gcctcccgtc cgtccgcctt ccttctctat cggcgagggc ttgagcgcgg gcgttccgcg
     6421 cgggcgttac gggccggttc gcgccgtctc gagcgcgcgg gccaggtcct cgggaagcgg
     6481 atcttcgaat gccagcggcg cgccgctcag cggatgcgcc agctcgaggc gcgcggcgtg
     6541 caggaacggc cgctccagac cgagccgcgc ggcgagcttc cggccccggc cgtagacctg
     6601 atcccccacg accgcgtggc cgatctcggc cagatgcacg cggacctgat gcgtgcggcc
     6661 ggtctcgata cgaacctcca gcagcgccgc ttcgccgagc cgctccatca cctgatagtg
     6721 cgtgatcgcc tcgcggccgc cggcgacgac ggccatcttc ttgcgatgcc gcggcgagcg
     6781 tccgatcggc gcgtcgatgg tcgcggtcgg gctctccggc tcgccttcga cgagcgcggt
     6841 ataggtgcgc ttgaccgttc ggctcgcgag catgccgacc aaagtcgcgt gcacgtgctc
     6901 gtctttggcc acgatcatca acccgctcgt tccggcatcg agccggtgaa cgatgccggg
     6961 ccgccaggcc tcgccgcccg ccggccggcc cgcgcgcgcc agcagcgcgt tgaccagcgt
     7021 tccgcccgga tgtcccggtg cgggatgcac gaccaggccc gccggcttag agatcaccac
     7081 gagcgcgtcg tcctcgtaga cgaccggaac gtcgatgtcc tcgggctgcg gctcggccgg
     7141 agccgcgtcg ggaaggtcgg cttcgacccg ctggccgcgc tcgagcttga gggacttgct
     7201 cgccggccgg tcgtcgaccg tcacgctcag cgccgcggcc gctgagcgcg acagcccggt
     7261 cctgcgcgcg agcaccacgt cgagccggtg gccggcttcc tcggccgcga cctcgaacga
     7321 gatgccgctc acgacgtcgc gccgccgtcg ccgccgcgcg cggccgcctc ggcggcgcgc
     7381 ttctcggcgc gttcggcgcg cacgccgtgc aggaacagca gggccgcccc ggtgaccacg
     7441 catacgtcgg cgatgttgaa agtcggccag atccggaagt cgatgaagtc gatgacgtgg
     7501 ccgcggaacg gcgccggcgg ccgcacgatc cggtcgctca ggttgccgag cgcgccgccc
     7561 agcacgaacc ccagcgccag gcccagcccg gggtcgtgca cgcggcgcgc ataccagatg
     7621 atgccggccg agatgacgaa ggcgatgatc gtgaacagcc acggcaccgt ggtgaacagg
     7681 ctgaaggccg cacccgggtt ccgctgcagc gtccaatgcc agacgccctc gatgaagggg
     7741 acggttccac cctcgggcag cttggccagg gcgagggcct tggtgatctg gtcggcgatc
     7801 agcccggcgg cagcggtgac cgacagcgcg aggagcgcgc gccagcttat ctgagtcgct
     7861 cttcctgctg tttgcacgtg atgcacaaca ccgagtacgg gagcgccttg agcctcgcct
     7921 tgtcgatggc gttgccgcac cgctcgcaaa caccatacga gccttcgtcg agcttgcgca
     7981 tcgcggtgtc caccttgtcc atcaggtcgc gaatgttgtt ggacagcgac aggtcgcgct
     8041 cacgctcgaa cgtgaacgtg ccggcgtcgg cgaactcctc gtcgtaggag acctccccgc
     8101 tcatctcgga ttgcgcgacg ttgagcgagc ccgactcgag atcctcgaac tccttctcgt
     8161 acgtggcacg gtggtcgagc agatcctgtc ggatcgcgtc cagctccttc ggagagaact
     8221 tgggaggctt gctcttgggc ttgggcgccg cgggggccgc ggcggccgcg ggcgttttgg
     8281 ccacgatctt cttggccggc gcaagcttgg tctcgctcgc aggcgccttc gcagcgggcg
     8341 tttccttctt ggcgggcaca gccttcgcag cgggcgcttc cttcttggcg ggcggagcct
     8401 tcttcgccgg cgcttccttc ttcgcagccg ccttctttgc gggcgcggtc ttcttcgcgg
     8461 gcgcggactt cttggccggg atcgtcttct tggacgacga tttgatcgga gccttcgcgg
     8521 acgtcttctt cgcggacggc ttcgccgccg cgcgcgccgc ctcggtcttc ttggcggcgg
     8581 cggtagcgct cgacttcttg gtggggcgag ccacccggct cctctcgtcc ttgcgtcgga
     8641 tcaccgctga gcggcgacga aacgggatat ccgaccgacc ccccggccga cggatgcccg
     8701 aaagtagcac cgcccccctg ggctgtcaag gcactgcagc ccggggcagc cggccccaac
     8761 ggcatcacat ttccgcaggt cagagcaggt gcggacgggc tccgtcgggg tcccgcggtc
     8821 ctgcgccccg ggcgtcacgg ggtaccgaaa gtgccgttcg cgatcgacac cacgttgatt
     8881 atgatgcgcg ctcacagatg agcgtctttc gaccggtcga ctcgcgcatc cgctggcccg
     8941 agatggagca gcgggtggcc gaacgctggc gcgaacgtga cgtgttcgcc cgctcgcagc
     9001 acctgcacga aggcggccgc cgatggaaca tctacgaggg cccgccgacc gccaacggca
     9061 aaccgggcgt gcatcatgtg ctcgcccgcg tcttcaagga cctgtttccg cggtaccgca
     9121 ccatgcgcgg cgatttcgtc ccgcgcaagg gcggctggga ctgccacggt ctgccggtcg
     9181 agctcgaggt cgagaaagag ctcggcctgt cgtccaagcg cgacatcgaa gccttcggca
     9241 tcgaggagtt caaccgccgc tgccgcgagt ccgtcctgcg ctacgtcggc gaatggcggc
     9301 gcatgaccga gcgcatcgcc tactggaccg acatggacga cccctactac acaatgtcca
     9361 acgactacgt ggagtcggtc tggtggtcgc tcaaaaagat ctacgacgac ggcctgctgt
     9421 tcgaggacta caaggtcacg ccctactgcg cacgatgcgg cacgccgctg tcggatcacg
     9481 aggtcgcgca gggctaccag accgtcaccg acccgagcgt cttcgtccgc ttcccgctca
     9541 ccgacgagac gggcacctcc ctgctggtct ggacgacgac gccgtggacg ctgctgtcga
     9601 atgccgcggc cgcgatccat cctgaggttc cctacgtcgc ggtgcagtac gagtcggagc
     9661 gcctgatcgt ggccgagccg ctcgtgcacc gcatcttcgg cgacgacgcc gaggtggtcg
     9721 agcacttcga gccgggatcg ctcgagggcc gctcgtacac gccgccgttc gatttcgccg
     9781 cccccgacaa gcgtgcctgg tacgtggtcg cggccgagta cgtcacgacc gaggacggat
     9841 cgggcatcgt gcacaccgcg cccgcttacg gcgccgagga catggagacg ggccgcgcga
     9901 acgacctgcc ggtgatccac atggtggatg ccgaaggcaa gttcgtggat gcggtcaccc
     9961 cctgggcggg ccagttcgtc aaggacgccg acccgtcgat catcgagaac ctgcgcgagc
    10021 gcggcatcct gttccgcgaa gagaccatcg agcacactta tccgttctgc tggcgctgca
    10081 agacgccgct gctctactac gcgcgcaact cgtggtacat ccgcacgacc gcgcgcaagg
    10141 acgatctgct cgcgcagaac gagaccatcg cctggcatcc cgaccacatc aagcacggcc
    10201 gcttcggcga ctggctcgaa aacaacatcg actgggcctt gtcgcgggac cgctactggg
    10261 gcacgccgct gccggtatgg cgctgcgagc agaagcacac ggtctgcatc ggctcgttcg
    10321 ccgagctggg cgaacgcagc ggccgcgacc tgtcgggctt cgatccgcac cggccgtacg
    10381 tggacgacat cacgttcgcg tgcgtcgagt gtggcggcga gatgcggcgc gtcaccagcg
    10441 tgatcgacac ctggtacgac tcgggctcga tgccgttcgc gcagtggcat tacccgttcg
    10501 acaacgagca gactttcgag gccgcgttcc ccgcgcagtt tatcgccgag ggcatcgacc
    10561 agacgcgcgg ctggttctat tcgctgctgg cgtcgtccac gttgctgttc ggccagtcgt
    10621 cctacgaacg cgtcatctgc cacggccaca tcgtggacaa ggacggccgc aagatgagca
    10681 agtcgctcgg caacgcgatc gacccgtgga cgatcctcga cggccaaggc gccgacgcgc
    10741 tgcggtggta cctgctgacc atcggctcgc cctggacgtc acgccgcctg tcgcttgatg
    10801 cgatcgacga ggtgctgcgc ggcttcatgc gtatgtggtg gaacgtatat tcattctacg
    10861 tgctctacgc caacaccgac ggcatcgacc ccgcgtcgct ggacgcgccg ccgccagccg
    10921 agcggggcga gctcgaccgc tggatcctgg ccgagctgca cgacaccgtg cgcggcgtca
    10981 ccgacgcgct ggaggccttc gacgccgccg acgccggccg ccgcatcacc gcgttcgtgg
    11041 acgagctgtc caactggtac gtgcgccgtt cacgccgccg cttctgggcg ggagccgagt
    11101 cggccgacaa ggccgccgcg cacgcgacgc tctacgagtg cctggtgacg atggcgaagc
    11161 tggtcgcgcc gttctgtccg ttcaccgcgg atgagatgtg ggacaaccta gtg
//
LOCUS       DATLFO010000138        16847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_23723, whole genome shotgun sequence.
ACCESSION   DATLFO010000138 DATLFO010000000
VERSION     DATLFO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23723"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VM841_05205"
     CDS             <1..327
                     /locus_tag="VM841_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89617.1"
                     /translation="VFDDDRDLGVAVALDGRIVGAAHYGDLDGDAEIAVIVEDGWQRH
                     GVGRVLMRDIADRAHADGYATATGCILRDNRAANRFLDATAPGARSVYDGPYRTFFVD
                     LSLIPG"
     gene            complement(324..752)
                     /locus_tag="VM841_05210"
     CDS             complement(324..752)
                     /locus_tag="VM841_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89618.1"
                     /translation="MPRLRMVALLAGLGLAAACGGDDGPAGAPGSASTPPAASPASSP
                     PPSVEPTQNGTYWAVYLAAGDPDSDAFVTRVEMLEARKLPSHGSEAACDQGSREALGL
                     KESDHVIGVYFTTEADARAFAATLDFEPAGIVQVKTDCLD"
     gene            complement(866..1489)
                     /locus_tag="VM841_05215"
     CDS             complement(866..1489)
                     /locus_tag="VM841_05215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVL89619.1"
                     /translation="MKVLTRILAVAIAFAVVAGGAQVYVSSRRPVPAAVKNPGAPAPG
                     PPSESPAPSQPPSDPVPPETLPPAPKPTKKPVTAPSKAQGKMIEVSIDDQELIAWQDG
                     TIVYRFVISTGKAGYETPKGNWRIHTKYENRWSRKWKVWMPYAMFWHPEHGYAFHELP
                     YKDGQPGKRLGASKLGRADSHGCVRVNVGDAKKLYDWAPVGTSVWVH"
     gene            complement(1665..2042)
                     /locus_tag="VM841_05220"
     CDS             complement(1665..2042)
                     /locus_tag="VM841_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL89620.1"
                     /translation="MTDGSAFRAAVEAEDHGAMVACLSPGVIFHSPVTFKPFEGRETV
                     AHLFGVLLSVFEDFTYTDHLTGDGTAILLFRTRVGDKQVEGADILRFGDDGLIEDFTV
                     MVRPLSAAMALGEAVGKGLGLRA"
     gene            2126..2542
                     /locus_tag="VM841_05225"
     CDS             2126..2542
                     /locus_tag="VM841_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89621.1"
                     /translation="MTFLRRQAFLLAIAVAASTGSPAGAHGGGASCGSDPAKPSTRWV
                     GSTSCHFEFRGAPLKVFGTATAASGEARLRIWISTTGGGPAIVECIASGTGETACANT
                     LGDQAPLLQLPPMRELWCNVEGTDSGDYRCLSALGI"
     gene            complement(2598..4412)
                     /locus_tag="VM841_05230"
     CDS             complement(2598..4412)
                     /locus_tag="VM841_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89622.1"
                     /translation="MARRLMTALAAVAVAFSLLPASADSGHRDVIPLLANAGNRYVPA
                     PPGTVQDLSYVFGPYVIPPGQDSNRITVDLPLTTGFIVAIAPDLVDVSTGEIPTQQEA
                     HIHHAHWFRVSEDSKNDYYTSVQGKGLSWVFGTGEEKTQGRLDDRARLDKAAGNDWEY
                     GIPISGSEPNTMIYMIHNKLATVGNYFVVLDVTFIPGTREEILAATNRDIHPLNSQLW
                     GRTKDVTGSSKEIGARWKVTRDGVGIASGGHLHPGGKMTVISNLGKDATCTADLDGDG
                     FPGVAILRSYKFDRDMRAWPFTENYQIGATKFGWRAPLHKGDVLEQFATYAIEPTGTP
                     ELNNFVSNDSLPHNWYEAMTYTGIYYDSYQVPGTPAGVCSLAALAPRLLGNDTFEEQG
                     LSSSWLDGDGLVKPELAAGVAEIEDDWNRFGTGAAEGMVNHIWQGEAEPLCAQENMPA
                     LAGIYPACGPDDIATELGPEVSEIHVAGFLYVPGDLGFGDVPTLPAVKQGSTITFVND
                     DAAINVRHTFTSCRWPCVGPYVSNFPLPSGGAHAFDTGKIGNIDPIDGGAEVEKVLLG
                     QENDTVPFYEFKADLPAGRYSYFCRIHPFMRGGFEVVT"
     gene            complement(4518..6533)
                     /locus_tag="VM841_05235"
     CDS             complement(4518..6533)
                     /locus_tag="VM841_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89623.1"
                     /translation="MASLFAMALSVASVRADLPVPEAGVISPQAGKVITGECKSESVF
                     DIDYDDPAIRPAAIKNSCQRLKIVFGPIWAKPGQNDVLIQPVTYEKPLYDGYMVRFKP
                     DLVGVDPLSSGFDANVAAPPVEDLHLHHGTWLNPSAFNDFGSGEFISTPAGNVPNPGR
                     RYGWGPWLASGEEKTIASWPEGFGLKIKSTDAWLFLHMVHNATPRTFPVWVTYDVDYI
                     PADVAEAPHPEDPERPLLFKTKGIWLDVGDCSHWTTTGCQKDQFNPVFNIQRGFGIDG
                     VCVFPRQNCAYQNTLANKSAQQGIDKGTVYDQWEIPEDGTLVVMGGHLHNGGLRDDVY
                     LVRDGVEKLIHRSDAFYFDHDFDPATWNEAVDPFDRTKSEIGAPPTSWDFVMSGVSDD
                     LGWRVEVKKGDKLRLEGVYDSSIASWYEQMGIVMTWLAPNSVTTIDGVDRVVGIDPFA
                     SGVEIHDGVNTLAVQPGTRPELIPGYEHGPCMPSATRLCTRGQITHARVATSGNHTDP
                     CLNGCPDIARTAPDGDFTSDIYMAAFGYGNADFGVIGALGVPRVKVGDTIAFHNVDTA
                     DYMWHTATRCANPCSGTVVTHYPIADGAYDDLIDPSTGTTANGKTVGDLLAENGADPL
                     DFDSGALGVGTGAVNKVTWSWTPTRPGTYTFWCRIHPSMRGAVRVVE"
     gene            complement(6650..7084)
                     /locus_tag="VM841_05240"
     CDS             complement(6650..7084)
                     /locus_tag="VM841_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89624.1"
                     /translation="MKKLLVAALVSLMAFTSSGASAQQIEHLYVSGPGGAFTTYTVPL
                     IVITQGDTITYRNIDIAAHDVRATVFGPDRAWCMQFPFNFPSGKCPLFTSRLIGLSGT
                     SPVYGIPDLVVPPSETAKTYEFFCSIHPNMKGTLVVVKRPLT"
     gene            complement(7099..7518)
                     /locus_tag="VM841_05245"
     CDS             complement(7099..7518)
                     /locus_tag="VM841_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89625.1"
                     /translation="MKALAAVIVLALAFPAQATARVVAAVPGSAQARTFATRVVPMQG
                     ATWLTLHNTDAAFHGIRSEAIGSDEASWCGPLNPGAEGPNNPRRFPRGACPLFWSDLA
                     LALGGARPVLGIESLLPGRAYGFICYVHPSMQGALLT"
     gene            complement(7515..9191)
                     /locus_tag="VM841_05250"
     CDS             complement(7515..9191)
                     /locus_tag="VM841_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVL89626.1"
                     /translation="MRTWRGIRSALAALLAVVSLSSLPSSARTICEPDARPGGEWRRM
                     GYDLAHSRHQPDERSIGRVQAATLQPAWTFSANEASGGFWNEITGYPIVADGCVFVGS
                     STGRDAPGWIFALNADGGDLSWKRALSHGVYSTLAVEDGVVYAFVSRIGSPYVVALDQ
                     RDGRMLWRTTVDTQPGSDAVSSPVVYNGMVWVGVSGTAAEGDEETRFDFRGSSVLLDA
                     SASCSDHSVPVAFGDETMTLTTECFNAVPGATGGRVLAHAFSIPDDDWDEGAGDAGGA
                     IWSTISIDPASGFGFVGTGNPFNYEDEHAHTNAIVKIDLRRTVAGSPNPSFGQIVGSY
                     KGEIEEYFPEVSDNYSCDEEDYVGFFMAGFECGRLDLDFGASPNLFRTRTGKLLVGAG
                     QKSGVYHAVDPVTMTGAWKQIMGVPSAVGGIVGTAAYDGTALYVPHTLAGYLASLDRD
                     GAGIKWASPTADAVHWGNPATSANGVIYTVDLKGFIDAFDAATGAPLMHRPLSLGANT
                     GLAPTLSWGGVTVARNTVYASVGVGLTSAGGGFPSMPNGFVIAYRPARTL"
     gene            complement(9194..10735)
                     /locus_tag="VM841_05255"
     CDS             complement(9194..10735)
                     /locus_tag="VM841_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVL89627.1"
                     /translation="MRARKLLIGLATLSMAILPAHAETVGHTCAPASHPGGEWRQSGG
                     SIGNHRNQPAETVLNASTAGNVAKAWVFSTAGAGTLQNTPVVADGCVFIATSTGWVMA
                     INADDGKAVWRTALPGSSAGALVGGIITGSPAVVDGVVYVATSELHHPRVVALDQNTG
                     AVKWAVEVLDPAKRPGGNPNRSYENTVVSAPVVWNGLLFQGIMAGEASASARGGYAIL
                     DIADTPALNANPDARRLVQDWTITDAEYAAGYRGASIWCTAAVDEATGYAYACGGNPA
                     SKQIEPRNANALLKIDMNRTRSTFGQIVDAYKGETDQYYPGLDRNPACDQVPTTAVWS
                     PTCVQLDLDFGASPVLFTDSIGRTLLGDLQKSGVFHAVYADNMQRAWTTVIATPGVAF
                     NAASPAVDTVSGNDGGMVFAAGSPPGHLVAMTKAFGRYRWATPISAGGTHFQPVSYAN
                     GVVYSMNNEGDLIGVDASLGLIVLKRNLAFDTGEWSGDLSSQGVAIARNTLYAASGQF
                     VVAYR"
     gene            10970..11899
                     /locus_tag="VM841_05260"
     CDS             10970..11899
                     /locus_tag="VM841_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL89628.1"
                     /translation="MGLLDGKVAIVTGAGRGIGRGEALLLAAHGARVIVNDVGGEWDG
                     SGADQRPAQQVVDEIAAAGGEAAANYDDVADFAAAGRMVQQAIDTWGRIDILVNNAGI
                     LRDKMIFNMDESDWDAVIRVHMKGHFAPTRHVCAHWRNLSKESGEAANGRIVNTSSTS
                     GLLGNVGQSNYGAAKAGIAAFAQIVSMEMRKYGVTVNAIAPAARTRMTENTFGDLMKA
                     GDFDQWDPENVAPIVCFLATDAAADITGQVFYTSGGIVQLYQGWNSIEEIRKDARWTV
                     EELVEKVPAMFRDKPKGYAPPQSLLRQDVTANK"
     gene            complement(12009..12371)
                     /locus_tag="VM841_05265"
     CDS             complement(12009..12371)
                     /locus_tag="VM841_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase"
                     /protein_id="HVL89629.1"
                     /translation="MSASRVLTVATGLWLMASPAVLGYGGAAAISDRIVGPWLVTLAI
                     VAMWESTRAVRLANLAFVPWLAVAPWVLSFPVRAAVVSLAVAGLIALTAFRTPMPRHL
                     FGGGWRSLWASPQRDAHS"
     gene            complement(12371..12817)
                     /locus_tag="VM841_05270"
     CDS             complement(12371..12817)
                     /locus_tag="VM841_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HVL89630.1"
                     /translation="MDVLDSVWEPFFGDGSEVILNSRVSHLLPIPDAALGAIGYLADA
                     GTGAIGGKDRWRTMPWIVIVFGLAVGPFGAVSILLVVLQPVMFDAWCTLCLASAGDLG
                     ADDRPRDGRGAREPATREAHARRAARQPGASSGDSSAEEIPQEQAA"
     gene            complement(12948..13100)
                     /locus_tag="VM841_05275"
     CDS             complement(12948..13100)
                     /locus_tag="VM841_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89631.1"
                     /translation="MSLTTVYLRALATFVSRRDERGAVGALILGIVIGIGLVVFGIIK
                     LLIPGD"
     gene            complement(13255..13677)
                     /locus_tag="VM841_05280"
     CDS             complement(13255..13677)
                     /locus_tag="VM841_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HVL89632.1"
                     /translation="MGEARTVMDAMTRAMFDGDMERAKSMYSTDAIGVAPDGSEFKGG
                     HEIVEFLSPFFRAFPDAEYQAIAQLESDKTAIDEGIFTGTHTEPMEALGGETIPPTNK
                     RISMRSCDVATVEGGKITEHHFYFDMLSMLEQLGISPS"
     gene            13860..14711
                     /locus_tag="VM841_05285"
     CDS             13860..14711
                     /locus_tag="VM841_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL89633.1"
                     /translation="MNKFELQSFDKPNETIEFELGRTEIVQIAEQTVGRTIFRPGWRW
                     SVHMRPHVGTDWCESRHVGLNLSGRFRFLLRDGTEHEVGPHEAYVVPAGHDGWVVGNE
                     PAVQLEWSGLRGWAGELESLAERVLATVLFTDIVDSTATAARLGDRRWQEVLAEFNSD
                     VRDALARFRGREIKTTGDGFLATFDGAARAIRCAALLAETAAAKDLRVRVAVHTGEIE
                     FVDQDARGVAVHEAARIMSLAGPQEVLVSEITRQLAAAANLAFEDRGEHRLKGLEGSR
                     RIYALVR"
     gene            14826..15986
                     /locus_tag="VM841_05290"
     CDS             14826..15986
                     /locus_tag="VM841_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89634.1"
                     /translation="MKARLLTAALALGIVGFGPAATALPANDNFADAQPVALNSSTST
                     STLGATRELGEPGHCNSASTVWYRFEPQDSSSVRIDTAGSNYDTTLAVYTGTASPPAW
                     DLELVDCNDDTSEDLTSAVGFQAEAGQTYYIQAGGFDGAQGSLVLTVQTAGAISGSVR
                     LEDGNPVDVCVRVHMPDGRDVGGTWAYGDFLIQGLPAGEFAVAFYPCGGQNFREEWWE
                     DKPSLGTADLVTVVAGQTTTGIDAVVAEFVPEPSPIDVALTSIRVENVPLRTDAATLP
                     GTGTVRDIVVTIENVGEAAAEGWVDIVACPKTGPFCQFAGYGFVSRLDPGQSRTVTVR
                     WNAAGGFGDFTIDAYGAFMDTTWQHYDRNWGNDWLTIEHYVILGGTGFGVGV"
     gene            complement(16074..16271)
                     /locus_tag="VM841_05295"
     CDS             complement(16074..16271)
                     /locus_tag="VM841_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89635.1"
                     /translation="MIFGLQIGGWFLAITAVMVFTVWAEAWLKSTATLKRVPVDRMSA
                     SAEVVEEVDVSSSARGWPRLP"
     gene            complement(16533..>16847)
                     /locus_tag="VM841_05300"
     CDS             complement(16533..>16847)
                     /locus_tag="VM841_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89636.1"
                     /translation="RASGHEGEVSQLGIVISLLVGAAGAILRYAVTGPANQQGFDIHT
                     GGVILMIVGGIGLVLSMLFWSGVAPFGRRDQSTRRHEEITTQDDHEQGRVNRETRDHV
                     AS"
BASE COUNT         2777 a         5622 c         5723 g         2725 t
ORIGIN      
        1 gtcttcgacg atgaccgcga tctcggcgtc gccgtcgcgc tggacggccg aatcgtcggt
       61 gccgcgcact acggcgacct ggacggcgac gccgagatcg cggtcatcgt cgaagacgga
      121 tggcagcggc atggtgtcgg ccgggtgctg atgcgcgaca tcgccgaccg cgcgcacgcg
      181 gacggctacg ccaccgcgac gggatgcatc ttgcgggaca atcgcgccgc gaaccgcttc
      241 ttggacgcga ccgcgccggg cgcacgcagc gtctatgacg gcccgtaccg gacgttcttc
      301 gtcgaccttt cgctcatccc cggttagtcc aggcaatccg tcttgacctg aacgatgcct
      361 gcgggctcga agtccagcgt tgccgcgaag gcgcgcgcat cggcctcggt cgtgaagtag
      421 acgccgatca cgtgatcgct ctccttcagg ccgagcgcct cgcgcgaccc ctgatcacac
      481 gcggcttcgc tgccgtgcga cggaagcttg cgggcctcga gcatctcgac gcgcgtcacg
      541 aacgcgtccg agtccggatc acccgccgcc agatacaccg cccagtaggt gccgttctgc
      601 gtcggctcta cggacggggg cggagacgat gcaggcgagg cggcaggggg cgtggacgcc
      661 gagccgggcg cgccggcagg accgtcatca ccgccgcacg cggcggccaa accgagcccg
      721 gcgagcagcg cgaccatccg caaacgtggc atggcgctca ccttagcgac ttcgatcacg
      781 cgcgcaaatg gcgcggggag gctccctctc aggaagcctc cccgcccggg tgggggcttg
      841 aggacgccgg cggcacgcgg gtccgtcagt ggacccagac ggacgtgccg accggcgccc
      901 agtcgtagag cttcttcgca tcaccgacgt tgacgcgcac acatccgtgc gagtccgcgc
      961 ggccgagctt cgacgcgccg agccgcttgc cgggctgccc gtccttgtag ggaagctcgt
     1021 ggaacgcgta gccgtgctcg gggtgccaga acatcgcgta cggcatccag accttccact
     1081 tgcgcgacca tcggttctcg tacttggtgt gaatcctcca gttgcccttc ggggtctcgt
     1141 acccggcctt gccggtcgag atcacgaagc ggtacacgat cgttccgtcc tgccaggcga
     1201 tgagctcctg atcgtcgatc gagacctcga tcatctttcc ctgggccttg gacggcgcgg
     1261 tcaccggctt cttcgtcggt ttcggcgcgg gcggcagcgt ctccggcggc accggatccg
     1321 acggcggctg cgacggcgcc ggcgactcgg acggcggccc cggcgcgggc gccccggggt
     1381 tcttcacggc ggcggggacc ggacggcggc tggacacgta gacctgcgcg ccgccggcga
     1441 cgaccgcgaa cgcgatggcg accgcgagga ttctcgtcaa gactttcatc ggacctgctc
     1501 cttcgtcgta cgcgtcgcgg gtctgcgtcg cgtatccata agacgctgcc ggcgagccac
     1561 gcggctacgg ccactcgggc acgtttcgcg tccggcaggt cattggacga aagggcagag
     1621 cacgcggttc cgtccccgcg gactgtgggt gccggcgcgt gcggttaggc gcgcagcccg
     1681 agacccttac cgaccgcttc gccgagcgcc atcgcggccg agagaggccg gaccatgacg
     1741 gtgaaatcct caatcaggcc gtcgtcgccg aagcgcagga tgtccgcgcc ctcgacctgc
     1801 ttgtcgccga cgcgcgtgcg aaacagaagg atcgcggtgc cgtcgcccgt caggtgatcg
     1861 gtgtaggtga agtcctcgaa caccgacaga aggacaccga acagatgcgc aaccgtctcg
     1921 cggccttcga acggcttgaa cgtgacggga ctgtgaaaga tcacgccggg cgacaggcac
     1981 gcgaccatcg ccccgtgatc ctccgcctcg acggctgcgc gaaacgcgga tccgtcggtc
     2041 atgaccgaag catatcgcct cggccggctc gagaatcccg gcgcgccggg caggccgccc
     2101 ggcggcagac gtcgaatgca cgatcatgac ttttcttcga cgacaggcat tcctcctcgc
     2161 gatcgcagtc gcggcttcga ccggcagccc cgccggcgcg cacggcggcg gagcaagctg
     2221 cggcagcgac ccggccaagc cgagcacccg ctgggtcggc tcgacctcgt gccatttcga
     2281 gttccgcggc gcgccgctca aagtgttcgg cacggcaacg gccgcctcgg gcgaggcacg
     2341 gctgcgcatc tggatctcga cgacgggcgg cggcccggcc atcgtcgagt gcatcgcgtc
     2401 cggcaccggc gagacggcat gcgcgaacac gctcggcgac caggcgcctc tgcttcagct
     2461 gccgccgatg cgcgagctgt ggtgcaacgt cgagggaacc gacagcggcg actaccgctg
     2521 cctgagcgcg ctggggatct gacgcggcga aatacgaaaa ggccgcccga gggcggcctt
     2581 ttctttgact ctgcgtgcta ggtgacgact tcgaatccac cccgcatgaa cgggtggatc
     2641 cggcagaagt acgagtagcg gccggcaggc aggtcggcct tgaactcgta gaacggaacg
     2701 gtgtcgttct cctggccgag caaaaccttc tcgacttcgg ccccaccgtc gatcgggtcg
     2761 atgttgccga tcttgcccgt gtcgaacgca tgcgctccgc cgctgggcag cgggaagttg
     2821 gaaacgtagg gtccgacgca cggccagcga cacgaggtga acgtgtgccg gacgttgatc
     2881 gcggcgtcgt cgttgacgaa ggtgatcgtc gagccctgtt tcacggcagg aagcgtcggg
     2941 acgtcgccga atccgagatc acccggcacg tacaggaagc ccgcgacgtg gatctcgctg
     3001 acctccggac cgagctcggt ggcgatgtca tcgggcccgc aggcgggata gatgcctgcg
     3061 agcgccggca tgttctcctg cgcgcacagc ggctcggcct cgccctgcca gatgtggttg
     3121 accatgcctt cagcggcacc ggtgccgaat cggttccagt catcctcgat ctcggcgacg
     3181 ccggcggcca gctcgggctt gacgagcccg tctccgtcaa gccatgacga cgacagcccc
     3241 tgctcttcga acgtgtcgtt gccgagcagc cgcggtgcga gcgcggccag cgagcagacg
     3301 cccgccggcg ttcccggcac ctggtaagag tcgtagtaga tgcccgtgta ggtcatcgcc
     3361 tcgtaccagt tgtggggcag cgagtcgttc gatacgaagt tgttcagctc cggcgttccc
     3421 gtcggctcga tcgcgtacgt ggcgaactgc tcgaggacgt cgcccttgtg cagcggagcc
     3481 cgccagccga acttggtcgc cccgatctgg tagttctccg tgaacggcca ggcgcgcatg
     3541 tcgcgatcga acttgtagga gcgcaagatc gccacgcccg ggaatccgtc accgtcgagg
     3601 tcggcggtgc acgtggcgtc cttcccgagg ttcgagatga cggtcatctt tccgcccggg
     3661 tgaaggtggc cgcccgaggc gatgccgact ccgtcgcgcg tgaccttcca gcgggccccg
     3721 atctccttgg agctgccggt cacgtccttg gtccggcccc acagctggct gttgagcggg
     3781 tggatgtccc gattcgtcgc ggcgaggatc tcctcacgcg tgccggggat gaacgtcacg
     3841 tcgagcacga cgaagtagtt gccgaccgtg gcgagcttgt tgtggatcat gtagatcatc
     3901 gtgttcggct cggatccgct gatcgggatg ccgtactccc agtcgttgcc ggcggccttg
     3961 tcgaggcgag cgcgatcgtc caggcgtccc tgcgtctttt cctcgccggt gccgaacacc
     4021 cacgacaatc ccttgccctg aacgcttgtg tagtagtcgt tcttggagtc ttccgagacg
     4081 cggaaccagt gcgcgtgatg gatgtgcgct tcctgctgcg tcggaatctc gccggtggac
     4141 acgtccacca ggtccggagc gatcgccacg atgaagccgg tcgtgagcgg cagatcgacc
     4201 gtaatacggt tcgagtcctg ccccggcggg atgacgtacg gtccgaagac gtacgacaga
     4261 tcctgcaccg ttcccggcgg tgccggcacg tatcgattgc ccgcgttggc gagcagcgga
     4321 atcacgtcgc gatgccccga gtcggccgaa gccgggagca gcgagaacgc gactgcgacg
     4381 gccgcgaggg ccgtcatgag tcgccgagcc atagatcccc ctccattctc ggtgctgctt
     4441 ctcctcattt ttcccctgcc atgaatgccg gagaggctgc cccgctgcgg cagcctctcc
     4501 ggcgttcgtt tcttccctta ctccacgacg cgaaccgcgc cgcgcatgct cgggtggatc
     4561 cggcaccaga acgtgtaggt gccgggacgt gtgggcgtcc acgaccacgt gaccttgttg
     4621 accgctccgg ttccgacgcc cagcgcgccc gaatcgaagt cgagcgggtc ggcgccgttc
     4681 tcggccagca gatcgccgac ggtcttgccg ttcgccgtcg tgcccgtgga cggatcgatc
     4741 aggtcgtcgt aggcgccgtc ggcgatcggg tagtgcgtga cgaccgttcc ggagcacggg
     4801 ttcgcacacc gcgtcgccgt gtgccacatg tagtcggcgg tatcgacgtt gtggaacgcg
     4861 atcgtgtcgc cgactttgac acgcggcacg ccgagcgccc cgatgacgcc gaagtcggcg
     4921 ttgccgtagc cgaacgcggc catgtagatg tcgctggtga agtcgccgtc cggagcggtg
     4981 cgcgcgatgt ccgggcagcc gttcaggcac gggtcggtgt ggttcccgct cgtcgccacg
     5041 cgggcgtgcg tgatctgccc gcgagtgcac aggcgcgtcg cgctcggcat gcagggcccg
     5101 tgctcgtagc cgggaatcaa ctcgggacgg gtgcccggct gaaccgcgag cgtgttgacg
     5161 ccgtcgtgga tctcgacgcc ggacgcgaac gggtcgatgc cgaccacgcg gtcaaccccg
     5221 tcgatcgtgg tgacgctgtt gggcgccagc caggtcatca cgatgcccat ctgctcgtac
     5281 cacgaggcga tgctgctgtc gtagacgccc tcgaggcgaa gcttgtcgcc cttcttgacc
     5341 tcgacgcgcc agcccaggtc gtcgctgacg cccgacatca cgaagtccca cgaggtcggc
     5401 ggtgcgccga tctcgctctt ggtccggtcg aacggatcga cggcctcgtt ccacgtggcc
     5461 gggtcgaagt cgtggtcgaa gtagaacgcg tcggaacggt ggatcagctt ctcgacgccg
     5521 tctcggacca ggtacacgtc gtcacggaga ccgccgttgt gcaaatgtcc gcccatgacg
     5581 accagcgtgc cgtcttcggg gatctcccac tggtcgtaga cggtgccctt gtcgatgcct
     5641 tgctgcgccg acttgttcgc cagcgtgttc tggtacgcgc agttctgacg cgggaagacg
     5701 cacacgccgt cgatgccgaa gccgcgctga atgttgaaca ccgggttgaa ctgatccttc
     5761 tggcagcccg tggtcgtcca gtggctgcag tcgccgacgt cgagccagat gcccttggtc
     5821 ttgaacagca gcggacgctc cgggtcttcg gggtgcggcg cctcggcgac gtcggccggg
     5881 atgtagtcca cgtcgtaggt gacccagacc gggaaggtgc gcggcgtcgc gttgtggacc
     5941 atgtgcagga acagccacgc gtcggtcgac ttgatcttca ggccgaaacc ttccggccac
     6001 gacgcgatgg tcttctcctc accggaggcg agccacgggc cccagccgta gcgacggccc
     6061 gggttcggca cgttccctgc aggcgtcgag atgaactctc ccgagccgaa gtcgttgaac
     6121 gcgctcgggt tgagccaggt cccgtggtgc aagtgaaggt cctcgaccgg cggcgcggca
     6181 acgttggcgt cgaagccgga cgacagcggg tccacgccga ccaggtccgg cttgaagcgg
     6241 accatgtagc cgtcgtagag cggcttctcg taggtcaccg gctggatcaa aacgtcgttc
     6301 tggccgggct tggcccaaat cgggccgaag acgatcttca agcgctgaca actgtttttg
     6361 atggctgcag gccggatcgc gggatcgtcg tagtcgatgt cgaaaacgct ttctgacttg
     6421 cattcaccgg tgatgacctt gccggcctgg gggctgatca cgcccgcctc gggcaccggc
     6481 agatccgctc tgacgcttgc tacggagagg gccatcgcaa agagcgaggc caaggctatg
     6541 acgcgtcttg tcactgtgat ccctcccgga tcctgtacgc ccgaaacccg gcgcaacgga
     6601 cgtcaaggca taagccgaca acattctcga ttctgactac tcggtaggat taagtcaagg
     6661 ggcgcttaac caccaccaac gttcccttca tgttcggatg gatggagcaa aagaactcat
     6721 acgttttcgc ggtttcgctg ggcggaacga cgagatccgg gatcccgtac acggggctcg
     6781 ttccgctaag gccgatcagc cgcgaggtga acagcggaca cttccctgac gggaagttga
     6841 acgggaactg catgcaccac gcgcggtccg gtccgaagac ggtggcccgc acgtcgtgag
     6901 ccgcgatgtc gatgttgcga tacgtgatgg tgtctccctg ggtgatgacg atgagaggca
     6961 cggtgtatgt cgtgaaggct ccgccgggac cgctgacgta cagatgctcg atctgctgag
     7021 cggacgcgcc cgacgaggtg aacgccatca gcgagaccag ggcagcgacg agaagtttct
     7081 tcattggagt cctttcgctt aggtgagcaa ggcgccctgc atcgatgggt ggacgtagca
     7141 gatgaagccg taggcccggc cgggcaggag cgactcgatc ccgagcacgg gacgcgcgcc
     7201 gccgagcgcg agcgcgagat cgctccagaa cagcgggcac gcaccgcgcg ggaagcggcg
     7261 cgggttgttg ggtccctccg cccccgggtt cagcggcccg caccacgagg cctcgtcgga
     7321 gccgatggcc tcggaccgta tgccgtggaa tgccgcatcg gtgttgtgca gcgtcagcca
     7381 ggtcgcacct tgcatcggca cgacgcgcgt ggcgaacgtg cgggcctgcg cgctgcccgg
     7441 caccgccgcg acaacgcgcg ccgtcgcctg cgccggaaac gccagcgcca gcacgatcac
     7501 ggccgcgagc gccttcacag cgttctcgcc ggccggtagg cgatcacgaa tccgttcggc
     7561 atcgacggga agccgccgcc ggccgacgtc aacccgacgc cgacgctcgc gtagacggtg
     7621 ttgcgggcga ccgtgacccc gccccaggac agggtcggcg caaggccggt gttcgctccc
     7681 agcgacagcg gacgatgcat cagcggggcg ccggtcgctg cgtcgaacgc gtcgatgaag
     7741 cccttgagat cgacggtgta gatcacgccg ttcgccgatg tggccggatt gccccagtgc
     7801 acggcatcgg ccgtcggcga agcccacttg atgccggccc cgtcacggtc cagcgacgcg
     7861 agatagcccg cgagcgtgtg cggcacgtac aaggcggtgc cgtcgtaggc ggcggtgccg
     7921 acgatacccc cgacggccga gggcacgccc atgatctgct tccaggcgcc ggtcatcgtc
     7981 accggatcga ccgcgtgata gacacccgat ttttggcccg cgccgacgag cagcttgccg
     8041 gtgcgcgtgc ggaaaaggtt cggcgacgcg ccgaagtcga ggtccagacg gccgcactcg
     8101 aacccggcca tgaagaagcc gacgtaatcc tcctcgtcgc acgagtagtt gtcggagacc
     8161 tcggggaagt actcctcgat ctcgcccttg taggagccca cgatctgtcc gaaggacggg
     8221 ttcggcgatc cggccaccgt gcggcgaagg tcgatcttta cgatcgcgtt ggtgtgggcg
     8281 tgctcgtcct cgtagttgaa cgggttgccg gtcccgacga agccgaagcc gctcgccgga
     8341 tcgatcgaga tggtggacca gatggcgccg cccgcatcgc ccgcgccttc gtcccagtcg
     8401 tcatcgggaa tcgagaacgc gtgcgcgagc acccggccgc cggtcgcgcc cggcaccgcg
     8461 ttgaagcatt ccgtggtcag cgtcatggtc tcgtcaccga acgcgaccgg cacgctgtga
     8521 tcgctgcacg acgccgacgc gtccagcagc accgacgagc cgcggaagtc gaagcgcgtc
     8581 tcctcgtcgc cctcggcggc cgttccggag acgccgaccc acaccatgcc gttgtagacg
     8641 accgggctgg agaccgcgtc cgatcccggc tgcgtgtcga cggtggtgcg ccacagcatt
     8701 cggccgtcac gctgatcgag cgcgacgacg tagggcgagc cgatgcgcga cacgaacgcg
     8761 tacacgacgc cgtcctcgac ggcgagcgtg ctgtagacgc cgtggctgag cgcacgcttc
     8821 caagacaggt cccccccgtc ggcgttgagc gcgaatatcc agcccggcgc gtcacggccg
     8881 gtgctcgatc cgacgaacac gcacccgtcg gcgacgatcg ggtagccggt gatctcgttc
     8941 cagaacccgc cgctggcctc gttcgccgag aacgtccacg ccggctgcag cgtggccgcc
     9001 tgcacccggc cgatcgagcg ctcgtccggc tggtggcgcg aatgcgcgag gtcgtagccc
     9061 atgcggcgcc actcgccgcc gggacgggcg tcgggctcgc agatcgtacg ggcggacgac
     9121 ggcagggagg acagcgacac cacggccagc agcgccgcca gcgccgatcg gatccctcgc
     9181 caggtccgca tggctagcgg tatgcgacga cgaactgtcc gctggcggcg tagagcgtgt
     9241 tgcgcgcgat cgccacgcct tgcgagctga ggtcgccgga ccattcgccg gtgtcgaagg
     9301 cgaggttgcg cttgagcacg atcaggccca gcgaggcgtc cacgccgatc agatcgccct
     9361 cgttgttcat cgagtacacg acgccgttcg cgtaggacac cggctggaag tgcgtgccgc
     9421 ccgcgctgat cggcgtcgcc cagcggtagc ggccgaacgc cttggtcatc gcgaccagat
     9481 gcccgggcgg cgagcccgcc gcgaagacca tgccgccatc attgcccgac accgtgtcta
     9541 cggccggcga ggcggcgttg aacgccacgc ccggcgtggc gatgaccgtc gtccaggcgc
     9601 gctgcatgtt gtcggcgtag accgcgtgga acacgcccga cttctgcagg tcccccagca
     9661 gcgtgcggcc gatcgagtcg gtgaacagca ccggcgaggc gccaaagtcc agatcaagct
     9721 gcacgcacgt cgggctccac acggcggtgg tcggcacctg atcgcacgcg gggttgcggt
     9781 ccaggcccgg gtagtactgg tcggtctcgc ccttgtaggc gtccacgatc tgaccgaagg
     9841 tcgagcgggt gcggttcatg tcgatcttca gcagcgcgtt ggcgttccgg ggctcgatct
     9901 gcttgctggc ggggttgccg ccgcatgcgt aggcgtagcc ggtggcctcg tccacggccg
     9961 cggtgcacca gatcgaggcg ccccggtagc cggccgcgta ctcggcgtcg gtgatcgtcc
    10021 agtcctgcac cagacggcgg gcgtccgggt tggcgttgag cgcgggcgtg tcggcgatgt
    10081 cgaggatcgc gtagcccccc cgcgcgctcg ccgaggcctc gccggccatg atcccctgga
    10141 acagcaggcc gttccagacc acgggcgcgc tcacgacggt gttctcgtag gagcggttcg
    10201 ggttgccgcc cggccgcttg gccgggtcga gtacctcgac ggcccacttc accgctccgg
    10261 tgttctgatc gagcgcgacc acgcgagggt ggtgaagctc gctggtggcg acgtagacga
    10321 ctccgtccac gacggcgggc gagccggtga tgatgccgcc cacaagcgct ccggcgctgg
    10381 agccgggcag cgcggttcgc caaacggcct tgccgtcgtc ggcgttgatc gccatgaccc
    10441 agccggtgct ggtggcgatg aagacgcatc cgtcggccac gacgggggtg ttctggagag
    10501 tgccggctcc tgctgtggag aagacccagg ccttggcgac gttgccggcg gtcgaggcgt
    10561 tgagaaccgt ctcggcgggc tggttgcggt gattaccgat cgagccgccg gactgtcgcc
    10621 actccccgcc cgggtgggag gcaggcgcgc aagtgtgacc gaccgtctcc gcatgggcgg
    10681 gcaggatggc catcgacagc gtcgcgagtc cgatcagcag cttccgagcc ctcatcgagc
    10741 gcctcctcct cagcgcggca gccgactgtt cggcgtgccc ctggatcgcg tgattccgac
    10801 gtaacggtcg gatttcgacg gataaggcgg ccgttcctgc ctgtcgaaga tcccggcaac
    10861 cgccgaatcc gagaaaaggg tgcgcatgtt ccggccagcg gaatttccca cacggcgagc
    10921 ggtcgatacg cttgcgcgcg acttcgggac ctgagtggag gatgcgagca tgggactgct
    10981 tgacggcaag gtggcgatcg tgaccggcgc gggccgcggc atcggtcgcg gcgaggcgct
    11041 gctgctggcg gcgcacggcg cgcgggtgat cgtcaacgac gtgggcggcg agtgggacgg
    11101 cagcggcgcc gaccagcgtc cggcgcagca ggtcgtggac gagatcgcgg cggccggcgg
    11161 cgaggccgcg gccaactatg acgacgtcgc cgacttcgcc gcggccggcc gcatggtgca
    11221 gcaagccatc gacacctggg gccggatcga catcctggtc aacaacgccg ggatcctgcg
    11281 cgacaagatg atcttcaaca tggacgagtc cgactgggac gccgtcatcc gcgtgcatat
    11341 gaagggccac ttcgcgccga cccggcacgt gtgcgcgcac tggcggaacc tgtcgaagga
    11401 gtccggcgag gcggcgaacg gacggatcgt caacacttcg tcgacgtcgg ggctgctggg
    11461 aaacgtcggg cagtcgaact acggcgccgc gaaggcgggc attgccgcgt tcgcgcagat
    11521 cgtatcgatg gaaatgcgaa agtacggcgt gaccgtcaac gcgatcgcgc cggccgctcg
    11581 cacgcgcatg accgagaaca ccttcggcga cctgatgaag gccggcgact tcgaccagtg
    11641 ggatcccgag aacgtcgcgc cgatcgtgtg cttcctggcg accgacgctg cggccgacat
    11701 caccggccag gtcttctaca ccagcggcgg aatcgtgcag ctctaccagg gatggaactc
    11761 gatcgaggag atccgcaagg acgcccgctg gacggtcgag gagctggtcg agaaggttcc
    11821 ggcgatgttc cgcgacaagc ccaagggcta cgcgccgccg cagtcgctgc tgcgccagga
    11881 cgtgacggcc aacaagtaac gccgtctccg gtgcgtccaa caaggagcgc ctctccgctc
    11941 gggctgctcg ttgttcttgg tcgattcaac gaacctataa cggcctcgga ttgaccaaga
    12001 acgagcgcct acgagtgcgc gtcacgctgc ggcgaggccc acaggctccg ccatccaccg
    12061 ccgaacaggt gccgcggcat gggcgtgcga aaggcggtca gcgcgatgag ccccgcaacc
    12121 gcgagcgaca cgaccgcggc gcgcacgggg aacgacagga cccacggagc gaccgcgagc
    12181 cacggcacga acgcaagatt cgccagtcgc acggcgcgcg tggactccca catcgcaacg
    12241 atcgcgagcg taaccagcca cgggcccacg atgcggtcgc tgatcgcggc cgcgccgccg
    12301 tagccgagca cggcgggcga ggccatcagc cacaggccgg tagcgacggt aagcacgcga
    12361 gatgcgctca tcaagcggcc tgctcttgcg ggatctcctc cgcgctcgag tccccagaag
    12421 acgcgccagg ttgacgcgcc gcccggcgcg catgcgcttc acgtgttgca ggctcgcgag
    12481 cacctcgtcc atcgcggggc cgatcatcag caccgagatc acccgccgac gccaggcaca
    12541 gcgtgcacca cgcgtcgaac atgaccggct ggagcacgac gaggaggatg ctgaccgcgc
    12601 cgaacggccc gacggccaat ccgaacacga tcacgatcca gggcatcgtg cgccagcggt
    12661 ccttcccgcc gatcgcgccc gttcccgcgt cggccaggta gccgatcgcg ccgagtgccg
    12721 catcggggat gggaaggagg tgggatacgc gcgagttcaa gatgacctcg ctgccgtcgc
    12781 cgaagaacgg ttcccagacg ctgtcgagaa cgtccatctg gtacagcgcc aggtacgcag
    12841 cgattgccgt gccgacgagc gcggagcgcg atgatcggca tgcgttgcgc ccaggtggaa
    12901 gggttgtagg accaccccgg cggaacggac gccctcatgc gaacggctta gtcgccgggg
    12961 atgagcagct tgatgatgcc gaacacgacc aggccgattc cgatgacgat gcccagaatc
    13021 agcgctccga ccgcaccgcg ctcgtcacgt cgtgaaacga aggtggcgag cgcccggagg
    13081 taaaccgttg tcagactcat gtggtgaaag tcccccgagc gagcatccgc taaacggccg
    13141 caacgccgtt cgtgcggcaa cggtgttcac aaactgccac gatttcaccg ggccgggcgg
    13201 gctggaagaa gccatggccg caggcggcga gtccgccacc ccctcccacc gcgtctacga
    13261 cggcgagata ccgagctgct cgagcatcga gagcatgtcg aagtagaagt ggtgctcggt
    13321 gatcttgccg ccttcgaccg tcgcgacgtc gcagctgcgc atcgagatgc gcttgttcgt
    13381 gggtggaatg gtctcgccgc cgagcgcctc catcggctcg gtgtgcgtgc cggtgaagat
    13441 gccttcgtcg attgcggtct tgtctgactc gagctgcgcg atcgcttgat actccgcatc
    13501 agggaatgcg cggaaaaacg ggctcaggaa ctccacgatc tcgtgaccgc ccttgaactc
    13561 gctcccgtcg ggcgcgacgc cgatggcgtc cgtcgagtac atcgacttcg ccctttccat
    13621 gtcgccgtcg aacatggccc tcgtcatcgc atccataacc gtgcgtgcct cgcccatgcc
    13681 acgctcctct ctcgctcgag ccgacgctag accgcgccgg tcccggcggc aatagcccga
    13741 tggagcccgc ccggccgggc accccacctt cgagcgcctg gcgttccgtg aacggcgcgg
    13801 ccccccggcg ccgatcggct ctaagatgag cggaaacgtc cggctatcgg gggcctgcaa
    13861 tgaacaaatt cgagctgcag agcttcgaca agcccaacga gacgatcgag ttcgagctgg
    13921 gccgcaccga gatcgtgcag atcgccgagc agacggtggg ccgcacgatc ttccggccgg
    13981 gatggcgatg gtcggtgcac atgcgtcccc acgtcgggac cgattggtgc gagagccgcc
    14041 acgtcgggct caacctgtcg gggcgcttcc gcttcttgct gcgcgacggc accgagcacg
    14101 aggtcgggcc gcacgaggcc tacgtggttc ccgccggcca cgacggctgg gtcgtcggga
    14161 acgagcccgc cgtgcaactt gaatggagcg ggctgcgcgg ctgggcgggc gagctcgagt
    14221 cgctggcgga gcgggtgctc gccaccgtgc tgttcaccga catcgtcgac tcgaccgcga
    14281 cggccgcccg gctcggcgac cggcgctggc aggaggtgct cgccgagttc aactccgatg
    14341 tccgcgatgc gctcgcgcgg ttccgtggcc gcgagatcaa gacgaccggc gacggctttc
    14401 tggccacctt cgacggagcg gcgcgcgcga tccgctgcgc ggctctgctg gccgagaccg
    14461 ccgccgcgaa ggaccttcgc gttcgcgtgg ccgtccacac cggcgagatc gagttcgtgg
    14521 atcaggacgc acgcggggtc gcggtgcacg aggcggcccg gatcatgtcc ctcgccggcc
    14581 cgcaggaggt tttggtctcc gagatcaccc gccagctcgc ggcggcggcc aacctggcgt
    14641 tcgaagaccg cggggagcac cggctcaaag gcctggaagg ttcgcggcgg atctacgccc
    14701 tggtccgctg acccattgcc atctccggtc ccttctgcga cactattgcc aattgcctgc
    14761 tacgcggctt ttacgaattc ttagggcaag gccgattcgg gggaaacgac ccacaagggg
    14821 gactcgtgaa agcacgcttg ctcactgccg cgctcgcgct cggcatcgtg ggcttcggtc
    14881 cggcagccac cgcgctgccg gccaacgaca atttcgcgga cgctcagccg gtcgcgttga
    14941 actcgtccac gtcgacgagc acgctgggag caaccagaga gctcggcgag cccggtcact
    15001 gcaactccgc cagcacggtc tggtatcgct tcgagccgca ggattccagc tcggtccgga
    15061 tcgacacggc cggcagcaac tacgacacga ctctggccgt gtacaccgga acggccagcc
    15121 cgccggcctg ggatctggag ctggtggact gcaacgacga cacgtcggag gatctcacct
    15181 cggcggtcgg tttccaggcc gaggccggac agacgtacta catccaggcc ggcggcttcg
    15241 acggggcgca gggatcgctg gtgctgacgg ttcagaccgc cggagcgatc agcggaagcg
    15301 tgcggctcga agacggcaac ccggtcgatg tctgtgtccg ggtgcacatg ccggatggcc
    15361 gcgatgtcgg ggggacatgg gcgtacggag acttcctcat ccaaggtttg cctgcgggcg
    15421 agttcgcggt cgcgttctat ccgtgcggcg gacagaactt ccgtgaagag tggtgggaag
    15481 acaagccctc gctggggacc gcggacctgg taacggtcgt cgcgggacag accacgacgg
    15541 gcatcgacgc ggtcgtcgcg gagttcgtgc cggaaccgtc gccgatcgat gtcgcgctca
    15601 cctcgatccg ggtcgagaac gtgccgctcc gcaccgacgc ggccacactg ccgggcaccg
    15661 gaaccgttcg cgacatcgtg gtcacgatcg agaacgtcgg cgaggcggca gccgaaggct
    15721 gggtcgacat cgtcgcctgt cccaagaccg gcccgttctg ccagttcgcc ggctacggat
    15781 tcgtgtcgag gctcgatccc ggccagtccc gcacggtcac cgtccgctgg aacgcagccg
    15841 gcggcttcgg ggacttcacg atcgatgcgt acggcgcgtt catggacacg acttggcagc
    15901 actacgaccg gaactggggc aatgactggc tgacgatcga gcactacgtc atcctcggag
    15961 gaaccggctt cggagtcggt gtgtagagca tcgcttcatc gaaagagccg cggcacgagc
    16021 cgcggctctt tcatgtccga tgcacggcct cgccgccgct cggcgcatcg atctcacgga
    16081 agccgaggcc atcctcgagc ggatgagctc acgtccacct cttcgacgac ctcggccgac
    16141 gccgacattc gatcgacggg tacgcgcttg agagtcgccg tcgacttaag ccaggcttcc
    16201 gcccacacgg tgaaaaccat gacggcggtg attgcaagaa accagccacc tatctgcagt
    16261 ccgaagatca tgacgtcact cctattcgag gttgatcgtt ccgtgtgctc accaaccgca
    16321 ctcgccgccc agctcccgct cgcagtgatg acagcggggg cctggagatt cgacgcgctg
    16381 gataccgcag tcccggcacg gctggctcgt gggtcctgcc ggcacgtcct cggcgggccg
    16441 tcgatccgcg gcgccgcgac cggcgaggat gccgaaccca acgggggaaa cgggttcgac
    16501 accggctcgc ctgaatgcgt cacgcatgga catcagctcg cgacgtgatc ccgtgtttcg
    16561 cgattgacac ggccttgctc atgatcgtcc tgcgtcgtga tttcctcgtg tcggcgtgtg
    16621 gattgatcgc gacgcccgaa cggcgcgacg ccgctccaga acagcatcga aagaacgagg
    16681 ccgattcctc cgacgatcat caagatcaca ccgccggtgt ggatgtcgaa gccttgctgg
    16741 ttggccggac ccgtgacggc gtagcgcaag atcgccccgg cggccccgac gagcaggcta
    16801 atgacgatgc ccaattgact cacttctcct tcgtgcccgg acgcgcg
//
LOCUS       DATLFO010000139         6765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_62235, whole genome shotgun sequence.
ACCESSION   DATLFO010000139 DATLFO010000000
VERSION     DATLFO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62235"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1145)
                     /locus_tag="VM841_05305"
     CDS             complement(222..1145)
                     /locus_tag="VM841_05305"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVL89637.1"
                     /translation="MLVPVTERGDAIGILELKTPYSPDNETLAYVTSAAHALAYTVIA
                     ARRHTDLFEWAQRDIPFSLPAEIQRRLLPDSYTVEGGPFTLAGWLEPAATVGGDTFDY
                     SVDREYLYASVTDAMGHSIGAAWLATLAVGSLRNARRSVASPSEQADAANAALIAAAA
                     PDQFVTGQIMRIRIADGAVDIVNAGHHPPYLLREGAAAALDLTPDPPLGIASDPYKSH
                     NLKLELGDRLLIVTDGFLERNADLAVGDILLQLADRHPREVVREFTKTLLAATGGDLR
                     DDATVLCIDWYGSRGVREATGGASLDRASSD"
     gene            complement(1485..2387)
                     /locus_tag="VM841_05310"
     CDS             complement(1485..2387)
                     /locus_tag="VM841_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89638.1"
                     /translation="MTKTRWIALISTTLLVGGCGSGADGGDGKVASQVGERATISHIH
                     GLGVDAHGTLFVATHDGLIKQGADGSFVYASADTNDHMGFSLHPGDGVMYRSGHSPAK
                     PSLGVQTSTDGARWMHVSDVADPPVDFHAMAVSFADSKTLWGWDSSGRGTFRSNDGGA
                     TWARLQSHGLEPQVYVLAGSAAPKVVYAGTASGLHRSSDGGATWERIVSGGWAIGVAT
                     DPKDPNRMLVSTQSGVKATSDGGDTWSDAGGGLPPSVEIAYLTISAADGNVAYAADSS
                     KIYKTTDGGTSWTRVPTDPSDPGP"
     gene            complement(2412..4370)
                     /locus_tag="VM841_05315"
     CDS             complement(2412..4370)
                     /locus_tag="VM841_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89639.1"
                     /translation="MRKLSILSVLVLVVAVLVPSLAAADSSDRYRGPYFGKNNFPPAC
                     TRDNAADNEHNLCYRMATGLNGLDSPQVDVLVMVPVSPTAERDMRILRQSVEMWEGGI
                     QTLAAQMGLDWLAEGVDFHITIDYVDPEGGGGEFTTYPVVDPEIVVIASNPVGGVGIG
                     IDPVDFASRVGLTDDGDAPCHPVSNPLDFEAWEALPGFDSHHGGRTGTYVEDCGGENG
                     NEGGGGNVCFAINGAIDPEPTALDVFNLFDLTSHEVGHCLTLGHVGDGGEAILGNGWG
                     AVPTNDIMSYNSDPDGLNKCVSTLDVEAFALRMSRYLDVNGDAVVDGADRLEANDQVG
                     SPDGLNPLQVQHPRDHFYASGSGAPTDCPQPDLGLIPGARTDWTPDPQPATIVPSLTV
                     ASPADGSTIDDGLFSVAGTVEYQINGEAAPSRPPSPDAPPPYDGTLTVRHRGAFEVEQ
                     STLGVTDQVPPIDSSHHYTLDLTNAHVAFELKWKDLFPFPPKSDLDLFVTGSGFDSGD
                     AGATAENPETIEIADATGHLDIRVRPTFVNTFTNYTLTITITPLDGGFGGLSPPDADL
                     DDVPDDSDACPWYPGTGSDGCTSMRPSEVAVYVDGAFAGLAEVHAEYWRDDFSIPITV
                     TEGEHRIRIDWLDGGNVLASRTLTVTVA"
     gene            complement(4619..5761)
                     /locus_tag="VM841_05320"
     CDS             complement(4619..5761)
                     /locus_tag="VM841_05320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVL89640.1"
                     /translation="MTGSKAGRWTDDAGRGATVPGAAHGSRTRRGRIGSMRATAMIAV
                     AMLMLATAPGAYSTSADGDYNPLPPASVWGRGDEAGNTNTQTPEKTLQAVSLIRSGEK
                     FLLGHVYDREMPLFPGNSWNLEAKPPLTIVQQVANTDYFHGEIGQNGTQFDSLGHFGI
                     HPSVTSPHETQYYNQWTHDEVHGPLGLKHLGVEKIKPFFTKAVLLDIARYANGGHTLS
                     PGQEITLDMVRQTLAAQSMSEHDISTGDVVLFRTGWEEFYDEGTLTYFAGAPGVPGAT
                     PGVGLAVARWLGTKRVACVGADNWGIEVVPAIDPPEGLIYPVHNELIVRNGIPLQESM
                     HLAGIAGDAAQRLRNGDPNPYTFAYVFVPVPVRGASGSPGIPIAIR"
     gene            complement(5758..6141)
                     /locus_tag="VM841_05325"
     CDS             complement(5758..6141)
                     /locus_tag="VM841_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89641.1"
                     /translation="MTEEAGSVVAGYLETMSTVWLAEDVTLVDARASRSYTGRAGALE
                     WLRRARLAEAQPSDIHLVVSGEQVAAEWLLRPQGGGAVLMAAFAVVEAGEISALRMYR
                     GGRGGRRVERDPRPARPAVEGGGAT"
     gene            complement(6199..>6765)
                     /locus_tag="VM841_05330"
     CDS             complement(6199..>6765)
                     /locus_tag="VM841_05330"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HVL89642.1"
                     /translation="TPPTVTCDQPTPVFLLNQQGAEVTAAVADEGSGPEAETVSAPTD
                     TSSVGAKSVALTGRDLAGNETTVECSYRVEYGFAGFFDPVKNPPAINTVTAGQVVPLN
                     FRLTDASGAPVTSLTTAKLTAVTLTCDLGTTADLPTEHVIGGLQNLVDGSYQLNWKTP
                     RIYAGSCKTLRLDLGEGAFRTALFRFRQ"
BASE COUNT         1077 a         2263 c         2251 g         1174 t
ORIGIN      
        1 cggagaaccc ttgcgtgcgc cgtcagtgtt tgcgcagacg atggaggttc tgctcgcgac
       61 gtattcggcc gtcttcttcg cagcgctggc cgccgctctg ggcgcgtact tcctcgaaca
      121 caagcacgaa accggatagc cccatctccc ctaagggccg acgaagtcac tcagactctg
      181 ctgggatgcg cgtcgcagcc gatcgaatgc ctgatcgcca gttagtcgga tgaggcccgg
      241 tcgaggctgg cccctccggt agcctcgcgg acaccgcgag acccgtacca gtcgatgcac
      301 aaaacggtcg cgtcatcccg gaggtcaccg ccggttgcgg cgagcagggt tttggtgaac
      361 tcgcggacga cctcgcgcgg gtgccggtcc gccagctgca gcagaatgtc acccacggca
      421 aggtctgcgt tccgttcaag gaacccgtcg gtcacgatta gaaggcgatc gccgagctca
      481 agcttcagat tgtggctctt gtacgggtcg ctcgcgatac cgagcggcgg gtccggggtc
      541 aggtcgagcg ctgccgcggc accctcccgc agcaggtacg gcgggtgatg gccggcgttg
      601 acgatatcga ccgcgccgtc ggcgatccgg atccgcatga tctggccggt tacgaactgg
      661 tcgggggctg ccgcggcgat cagagcggcg ttcgccgcgt ctgcctgttc gcttggcgac
      721 gccacggacc ggcgggcgtt gcggagactc ccaaccgcaa gcgtggcgag ccaagcggcg
      781 ccgatggaat ggcccatggc gtcggtgacg gacgcgtaca ggtactcacg atcgacggag
      841 tagtcgaagg tgtcgccgcc cacggtcgcc gccggctcga gccagccggc gagggtgaag
      901 gggccgcctt cgaccgtgta ggaatcgggc agcagccgac gctggatctc ggccgggagc
      961 gaaaacggaa tgtctcgctg cgcccactcg aataagtcgg tgtgacggcg ggccgcaatg
     1021 acggtgtacg cgagtgcgtg cgcggcggac gtcacgtacg caagggtctc gttgtcgggg
     1081 ctgtacggag tcttgagttc gaggatgccg atcgcatcgc cgcgctcggt caccggaacg
     1141 agcacgcgcc agcctccgtc gacacgctca acatgttgct cctggctcgt gaggactcga
     1201 tgatggacgg atccgtcgag cggaaccgac tgcgtccgct cgttacgccc ggcggcgacc
     1261 gcgccttctc tggtgacgtt cgacatgcgc atggcggcgg tccccgaaag gttggcgacc
     1321 agaaggctaa cctcggtcgc gtccagcatc cgggcgagct cgtgggccag aacgtcgacg
     1381 acctcgaccg gcggggcagc ctcgacggct gcaagcagac cccggaggtc gagtcggacg
     1441 tcgtctgcgt cggccaccct cataccgtag cagcaaccgt aatttcatgg cccgggatca
     1501 gaaggatctg tcggcacccg ggtccacgac gttccgccgt ccgtggtctt gtaaatcttg
     1561 gacgagtcgg ccgcgtaggc cacgttgccg tcggcggcgc tgatcgtaag gtaggcgatc
     1621 tcgacgctcg gcggcagccc gccgcccgcg tcgctccatg tgtctccacc atccgacgtt
     1681 gccttcaccc ccgactgcgt cgagacgagc atccgattcg gatccttcgg atccgtcgcg
     1741 acgccgatcg cccatccacc cgagacaatg cgctcccagg tcgcaccgcc gtcgctcgac
     1801 cgatgcaggc cgctcgccgt ccccgcatag acgaccttcg gcgcggcaga cccggcaaga
     1861 acgtagacct gcggttcgag gccgtgcgac tgcaggcgcg cccaggtcgc gccgccgtcg
     1921 tttgagcgga acgtgccgcg gccactcgag tcccaccccc acaatgtctt ggagtcggcg
     1981 aagctcaccg ccatcgcgtg gaagtccacc ggcgggtcgg ctacgtcgga cacgtgcatc
     2041 cagcgcgcgc cgtcggtcga ggtctgcacg cccagcgacg gcttcgccgg cgaatgcccg
     2101 ctccggtaca tcactccgtc accgggatgg aggctgaatc ccatgtggtc gttcgtgtcc
     2161 gcactcgcat agacgaacga tccgtcggca ccctgcttga tcagcccgtc gtgcgtcgca
     2221 acgaagagcg ttccgtgcgc atcgacgccg agcccgtgga tgtgcgagat cgtggcacgc
     2281 tccccaacct ggctcgcgac tttcccgtcg cctccgtccg ccccggagcc gcacccgccc
     2341 acgagaaggg tcgtgctgat gagcgcgatc catcgggtct tcgtcatgca ccaccaaccg
     2401 ggtgatcggc gctatgcgac ggtgacggtg agcgtgcggc tggcgagaac atttccgccg
     2461 tcgagccagt cgatgcgaat tcggtgctcg ccttcggtga cggtgatggg tatcgagaag
     2521 tcatcccgcc agtactcggc atggacttcg gcgagacccg caaaggctcc gtccacgtac
     2581 acggcgacct cgctgggtct cattgacgtg cacccgtcgg atccggtccc cggataccac
     2641 ggacaggcat cgctgtcgtc gggtacgtcg tcgaggtccg cgtccggcgg agacaggccg
     2701 ccgaaccccc catcgagcgg ggtgatggtg atggtcagcg tgtagttggt gaacgtgttg
     2761 acgaaggtgg gccggactcg gatgtccagg tgaccggtcg cgtcagcgat ctcgatcgtt
     2821 tccgggttct ccgcggtggc accggcgtcg ccggaatcga agccacttcc ggtcacgaac
     2881 aggtcgagat cgcttttcgg cgggaaggga aacaggtctt tccacttcag ctcgaacgct
     2941 acgtgcgcgt tggtgaggtc gagggtgtaa tggtggctgc tgtcgatcgg cggcacctga
     3001 tcggtcaccc cgagcgtgga ctgctccact tcgaacgcgc ctcgatgccg aaccgtgagc
     3061 gtgccgtcgt acggcggcgg cgcatcggga gacgggggac gcgacggtgc ggcctcaccg
     3121 ttgatctggt actcgacggt gcctgcgacc gaaaagaggc cgtcgtcgat cgtcgagccg
     3181 tccgccggcg acgcgacggt gagcgacggc acgatggtcg cgggctgcgg atcgggcgtc
     3241 caatcggtcc gcgcgccagg gatcaggccc aggtcgggct gcggacagtc ggtcggcgca
     3301 cccgagccgg aggcgtagaa gtggtctcga ggatgctgaa cctggagcgg gttgaggccg
     3361 tcgggcgatc cgacctggtc gttggcctcg aggcggtccg cgccatcgac gacagcgtcg
     3421 ccgttgacgt cgaggtatcg actcatgcgg agggcgaagg cctcgacgtc gagggtcgac
     3481 acgcatttgt tgagcccgtc cgggtcgctg ttgtaggaca tgatgtcgtt ggtcggaacg
     3541 gctccccatc cattgcccag aatggcctca ccgccgtcgc cgacgtggcc gagtgtcaag
     3601 cagtgaccga cttcgtgtga ggtgaggtcg aagagattga aaacgtcgag cgccgtcggt
     3661 tcgggatcga tggcgccgtt gatcgcgaag cagacgttgc ccccgccccc ttcgttgccg
     3721 ttctcaccac cgcagtcctc gacgtaggtt ccggtgcggc ccccgtgatg actgtcgaag
     3781 ccgggcaggg cttcccatgc ctcgaaatcc agagggttcg agaccgggtg acacggcgcg
     3841 tcgccgtcgt cggtcagacc cacgcggctc gcgaaatcca ccggatcgat gccgatgccg
     3901 acaccgccaa cggggttcga ggcgatgacg acgatctcgg gatccacgac cgggtaggtc
     3961 gtgaactcgc cgccgccgcc ttccgggtcg acatagtcga tcgtgatgtg gaagtccacg
     4021 ccttcggcga gccagtcgag tcccatctga gccgcgaggg tctggatccc gccttcccac
     4081 atctccaccg actgtcgcag aatccgcatg tcacgctcgg cggtcggcga taccggaacc
     4141 atcaccagca cgtccacctg cggggagtcc agtccgttga gcccggtcgc catgcgatag
     4201 cacaggttgt gctcgttgtc ggcggcattg tcacgggtgc aggcgggagg aaagttgttt
     4261 ttaccgaagt acggtccccg atatcgatcg ctgctgtcgg ccgcggcgag cgatgggact
     4321 aagacggcga cgacgagcac cagcacgctc aagatcgata gcttgcgcaa cgttctccct
     4381 ccggatgccg ggactcccca attgtctggg acctgtgcct ggtgacttcg ccccgagaga
     4441 aagactctcc tgctaatcgg cccgacgcgg cacctgccgc ggtggtcgaa gatggtggcc
     4501 atcgctcgag ttgagctacg atgcgcgacg tcgtagcgac cctgccgtcc aggccgccgc
     4561 gactcgctca accctgcgca agctccgtta gcaccccacg tggtaccggc gctccacgct
     4621 atcggatcgc gatcgggatc cctggagatc ccgatgcacc gcgaaccggc acgggaacga
     4681 acacgtaggc gaacgtatag gggttcgggt ctccattccg cagccgctgt gccgcgtccc
     4741 ccgcgatccc ggcgaggtgc atcgactcct gaagcggaat gccgttgcgc acgatcagct
     4801 cgttgtgtac cggatagatc agaccctccg gcgggtcgat cgcaggcacg acctcgatcc
     4861 cccagttgtc ggcgcctacg caggcaaccc gcttggtgcc gagccagcgc gcgaccgcga
     4921 ggcccacccc cggcgtcgcg cccggtacac ccggcgcgcc cgcgaagtac gtgagagtcc
     4981 cctcgtcgta gaactcctcc catccggtcc tgaagagcac aacgtccccc gtcgagatgt
     5041 cgtgctcgct catcgactga gcggcgagcg tttggcgcac catgtcgagc gtgatctcct
     5101 ggccgggaga caatgtgtgg ccgccgttcg cgtaccgagc gatgtcgagc agaaccgcct
     5161 tcgtgaagaa cggcttgatc ttctcgacgc cgaggtgctt gagaccgaga ggaccgtgga
     5221 cctcatcgtg cgtccactgg ttgtagtact gagtctcgtg cggcgaagtg accgacggat
     5281 ggatgccgaa gtggccgaga ctgtcgaact gagtgccgtt ctgcccgatc tcaccgtgga
     5341 agtagtcggt gtttgctacc tgctgcacga tcgtcaaggg aggtttggct tccaggttcc
     5401 atgagttgcc cgggaagagc ggcatctcgc ggtcgtaaac gtgtcccagc agaaactttt
     5461 cccccgagcg gatcagtgat acagcctgga gcgttttctc cggcgtctgc gtgttcgtgt
     5521 tgcctgcctc gtcgccccgc ccccaaaccg acgccggcgg cagtgggttg tagtcgccgt
     5581 ccgccgacgt cgagtacgca cccggcgccg ttgcgagcat gagcatcgcg acggcgatca
     5641 tcgcggtcgc gcgcatcgac ccgatacggc cgcgtcgtgt gcgcgacccg tgcgccgcgc
     5701 cgggcacggt ggcgccccgt ccggcgtcgt cggtccatcg tcctgccttg gaacctgtca
     5761 tgtcgctccc cctccttcga ccgccggccg tgccggtcgc gggtcccgct cgacccgccg
     5821 ccccccgcgg ccgccgcggt acatgcgcag cgccgagatc tcgccggcct cgacgacggc
     5881 gaaagcggcc atcagcaccg ctccgcctcc ctgcggccgc agtagccatt ccgccgcgac
     5941 ctgctcgccg gagaccacca gatggatgtc gctgggctgc gcttccgcca ggcgcgcccg
     6001 gcgcaaccac tcgagggctc cggcgcggcc cgtgtacgac ctgctcgcgc gcgcgtcgac
     6061 cagcgttacg tcctcggcca gccataccgt cgacatagtt tcgaggtagc ccgccacgac
     6121 gctgcctgct tcctccgtca attcgctctc cttgtttcgg tttcgatggc gcacccacga
     6181 cgccggcacg cgccggacct actgcctgaa ccggaacagc gccgtccgga acgccccctc
     6241 gccgaggtcc agccgcagcg tcttgcagga gccggcgtag attctcgggg tcttccagtt
     6301 gagctggtag gagccgtcga cgagattctg cagaccgccg atgacgtgtt cggttggcag
     6361 gtcggcggtg gtcccgagat cgcaggtgag cgtcaccgcg gtgagcttgg ccgtggtcag
     6421 gctcgtaacc ggggcgcccg aggcgtcggt cagccggaag ttgagcggca ccacctgccc
     6481 ggccgtgacg gtgttgatcg ccggcgggtt cttcaccgga tcgaagaacc ccgcgaagcc
     6541 gtactcgacc cgataggagc actcgaccgt ggtctcgttg ccggccaggt cgcggcccgt
     6601 gagcgcgacg gacttggccc cgaccgagct cgtatcggtc ggcgcgctca ccgtctcggc
     6661 ctcgggcccc gacccttcgt cggcgacggc cgccgttacc tccgccccct gctgattgag
     6721 caggaagacg ggcgtgggct gatcgcaggt gacggtgggc ggcgt
//
LOCUS       DATLFO010000140         5781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_121793, whole genome shotgun sequence.
ACCESSION   DATLFO010000140 DATLFO010000000
VERSION     DATLFO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121793"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VM841_05335"
     CDS             <1..244
                     /locus_tag="VM841_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89643.1"
                     /translation="ALVSELSTWLRLFGDESVVELDYGGLASLVPAGELEEDHSAADL
                     REAVEALGEGDVLRATTAYMRAAERWAPLSAMERTN"
     gene            689..889
                     /locus_tag="VM841_05340"
     CDS             689..889
                     /locus_tag="VM841_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HVL89644.1"
                     /translation="MKRDQTSGETLLTPAEVAALFRVDPKTVTRWAKAGKLSSIRTLG
                     GHRRYRESEVMSLLEGVAPKQG"
     gene            complement(1044..1116)
                     /locus_tag="VM841_05345"
     tRNA            complement(1044..1116)
                     /locus_tag="VM841_05345"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1081..1083),aa:Phe,seq:gaa)
     gene            complement(1181..1255)
                     /locus_tag="VM841_05350"
     tRNA            complement(1181..1255)
                     /locus_tag="VM841_05350"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1218..1220),aa:Asp,seq:gtc)
     gene            complement(1314..2159)
                     /locus_tag="VM841_05355"
     CDS             complement(1314..2159)
                     /locus_tag="VM841_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89645.1"
                     /translation="MTWLRDRTFGAESVPTLSGLRARKHATGSAVAVVIAGTPADATA
                     RAIRACVQSDLIDDAGLVDEIVISDPDGGGRGFALRQSLAAIRSDIVLFLDGGIRNFS
                     PRIAVRMIAPLLLEEQLVFIKGFAPSISPKSGGRAADHGMARPLLAASFPELDAFEQP
                     FARETAARTALLRRLAFPPGEGVDAALLIDALEAAGAGALGQSDLGGSIHRTTPVSHY
                     DGDIAMTVLRRAGERGRIRPFADATVYPLLVRDRASEQPDLPPIESGHAVRRSRRPRP
                     ARVAN"
     gene            complement(2428..2994)
                     /locus_tag="VM841_05360"
     CDS             complement(2428..2994)
                     /locus_tag="VM841_05360"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HVL89646.1"
                     /translation="MRRRVLVAATLCLAVACGGTSGPVTIPTSDVPFPLTREAAQPEV
                     TSTVGRILVFFVLDGRLSAVTRTAEAGDTPTEASMRALLGGPNSREQERGLTTNLPAS
                     TRLLDITIADRVAVADLSAEFQAPAEPDAITLRIAQVVWTLTQLPGVEAVRFAVDGNL
                     ISVATDDGQSVDRPVSRADYDQFAPLGR"
     gene            complement(2991..4379)
                     /locus_tag="VM841_05365"
     CDS             complement(2991..4379)
                     /locus_tag="VM841_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL89647.1"
                     /translation="MRSPLALLRRRSLRLRVTIAFVATAAILVTALSLVTLLTVRSFM
                     ESQRVRSSNRQTVFALLFAREFVRAEPNAAELVTRLQIRQSFDAMVTDHGEWFATSLA
                     LTPDAVPSGLRAIVRNERLGYQIAQPPGSNRLLIFGSPLPPPGMDLYLFFPLEDVDRT
                     ISLLGRVMAVAGLLIVVVVTLVARRVTSGIMHPLAAVSDATRRMAEGLLETRVTPTSQ
                     DEVGQLATSFNRMAEALRGMIERERYFVAAASHELRTPLAALQASADVLAAHREHLSA
                     EGREALDLIREDIGALNLLVRELLEISELDAGRAPIRIEPVRLRAFSEALLHRRRYDA
                     RLSGDDHTVETDKARLERVLGNLIDNAYTHGGGHDVRVEIGRDDGAYLISVSDAGPGV
                     PAADQEKLFTRFYRSDISRSRERGGVGLGLAIAQENARLLGGAIAVTSGNGRTTFTLR
                     LPERSPGDGGGE"
     gene            complement(4376..5059)
                     /locus_tag="VM841_05370"
     CDS             complement(4376..5059)
                     /locus_tag="VM841_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89648.1"
                     /translation="MDATILLVDDDRRLRRSIALALGQEGLRTLEAEDADEALALLEK
                     DKPDLAILDVMLPRRDGFELCREIRKSSDMPVIFLSAKTDTVDVVVGLESGGDDYVTK
                     PFETRELIARIKALLRRVQSEAPMRKMSFGALEIKPESGVVLKAGERVPLTKTEFKLL
                     CLLASRPDVIFTREVLLEQVWGYDYLGDSRLVDTHVRRLRAKIESDPAHPALVQTVRG
                     LGYKLSSEP"
     gene            5186..5440
                     /locus_tag="VM841_05375"
     CDS             5186..5440
                     /locus_tag="VM841_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HVL89649.1"
                     /translation="MSQTYVLVEVEPGAIDRVLDELVLLPGVAEATCVVGGFDLIIRA
                     EVADEALSASLRAHIASMRGVTRALPCEVRREPVGVASAL"
     gene            5534..>5781
                     /locus_tag="VM841_05380"
     CDS             5534..>5781
                     /locus_tag="VM841_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVL89650.1"
                     /translation="MTGPVEIHHLIAAGSSLAGGLLLGLFIYRLVLRPLVKHSTLTQA
                     GADDVILPAIRKATVLWFAVAGAFAARLSLPLAAGPARV"
BASE COUNT          905 a         1947 c         2024 g          905 t
ORIGIN      
        1 ggctctcgtg tcggagctgt ccacctggct ccggctcttc ggcgacgagt cggtcgtgga
       61 gctcgactac ggcgggctcg cgtcgctggt gcccgccggc gagctcgagg aggaccattc
      121 cgccgcggat ctccgtgagg ccgtcgaggc cctcggggag ggagacgtgc tccgggcgac
      181 caccgcttat atgcgcgccg cggaacggtg ggccccgttg tccgcgatgg agcggaccaa
      241 ctagtccctt ccccgcacct cgctcatctc gactttcccg cttgttttgc ggggtttcca
      301 ggccctcccg ggcaactttc atgaccattt agtacatatt tattccgttt ttgtggtact
      361 atggtcgaca tgatgtggga tgtttcctgc cgacttgggg aaatttcacc caggagcgct
      421 ttccttttcg ggtagagcga ccgatatttg tggaacggaa tcgaacgacg ctcgttcatt
      481 acgggcgaag gacttgaaaa ccggatggac accgcaaggg gtccaaaggg actagggccg
      541 aaactacccg gatgagtcat ttacccggag ggcatttcgg gacgacaatc cggacaggaa
      601 gtaatcagca acgacaagac gtcaccagcg aagacaatcc gcggcagacc tgcgagcttc
      661 cgcggagaat cagcaaggag ggaaccatat gaaacgggac cagacttccg gtgagacgct
      721 tctcaccccc gcggaggtag ccgctctgtt ccgcgtcgac cccaagacgg tcacgcgctg
      781 ggcgaaggcc ggcaagctct cttcgattcg cacgctcggc ggtcaccgcc gctaccgcga
      841 gtccgaggtc atgagcctgc tcgagggcgt cgcaccgaag cagggataag caccagaacc
      901 atggcgagac catgccgatc gggtcccccc gagcggcatg gctgagaccg aaaagagacg
      961 aggggcgcgg cagaggcgcc cctcttcttt tttgcctttt gccttcgaaa aggcgaaggg
     1021 agcccgatcg ggctcccatc cggtggccag gggcagaatc gaactgccga caccggcatt
     1081 ttcagtgccg tgctctgcca actgagctac ctggccggct cggatcgccg aaacgacccg
     1141 tttcactgcg acggccccgg aatgaaccgg ggccgcgtcg cgcccgcgac cggacttgaa
     1201 ccggcgacct tcaccttgac agggtgacga gcactcctaa ctgctccacg cgggcgtggg
     1261 acaaggcagt gtacccgacc ggcgcctcgg acggcactcc ggaaacggca aagtcagttc
     1321 gcgacccggg ccggccgggg ccggcggctg cgccggacgg cgtggccgga ctcgatcggg
     1381 ggcagatcgg gctgctcgga ggcccggtcg cgcacgagga gggggtagac ggtcgcgtcg
     1441 gcgaacggcc gtatccgacc ccgctccccg gcgcgccgaa gaacggtcat cgcgatgtcg
     1501 ccgtcgtagt gcgacaccgg cgtcgtgcgg tgaatgctgc cgcccaggtc cgactggccg
     1561 agggcgccgg caccggccgc ctcgagcgca tcgatcagca gcgccgcatc gaccccttcg
     1621 cccggcggga acgcgagccg tcgcagcagc gcggtgcggg ccgcggtctc gcgggcgaac
     1681 ggctgctcga aagcgtcgag ctcggggaag ctagccgcga gcaggggacg cgccataccg
     1741 tggtcggcgg cgcgtccccc gctcttgggg gagatcgacg gtgcgaagcc tttgatgaag
     1801 acgagctgct cttcgagcag cagcggggcg atcatgcgca cggcgatgcg cggagagaag
     1861 ttgcggatgc ctccgtcgag gaacagcacg atgtcgctgc ggatcgccgc caggctctgg
     1921 cgcagcgcga agccgcggcc gccgccatcc gggtccgaga tcacgatctc gtcgaccagc
     1981 ccggcgtcgt cgatgaggtc gctctgcacg caggcccgga tcgcgcgggc cgtcgcgtcg
     2041 gccggcgttc cggcgatcac gacggctacc gcggatccgg tggcatgctt gcgggcacga
     2101 aggccggaca aggtcggcac cgactcggcg ccgaaggttc ggtcccgcag ccaggtcaac
     2161 ggcccgtgcc tccgacctcg gcgccgcacc gcacgcagac cgcacgcccg gtcgtcggat
     2221 cgatgatccg tccttcgacc ccggtggcga cgtgtccgca gtgcggacac gtctcgtgcg
     2281 gcggctccgg ccgcgcggcc gcagggccgg accgttccga ggacgcgcga cgccgcaggt
     2341 tcacggatcc atcatcggag cggctggtgg cgaacgggtt gcggcaaagt tacggttcgg
     2401 cgtcgaatac gggccgcgcg gggacggtca gcggccgagc ggggcgaact gatcgtagtc
     2461 ggcccgcgag acggggcggt ccacggattg gccgtcgtcg gtggcgacgg agatcaggtt
     2521 gccgtccacc gcgaatcgaa ccgcctccac gcccggcagc tgggtcagcg tccacacgac
     2581 ctgggcgatg cggagcgtga tcgcgtcggg ctcggccggc gcctggaact ccgcgctcag
     2641 gtcggcgacc gcgacccggt cggcgatggt gatgtcgagc agcctggtcg acgctggcag
     2701 attcgtcgtc aggccgcgct cctgctcccg gctgttgggt cccccgagca gtgcgcgcat
     2761 ggacgcttcc gtgggcgtgt cgcccgcctc ggccgttcgc gtcaccgcgg acaggcggcc
     2821 gtcgaggacg aagaagacga ggatgcgtcc cacggtggac gtgacctccg gctgcgccgc
     2881 ttcgcgcgtg agcgggaatg gcacgtccga ggtcgggatc gtcacgggtc cggacgtgcc
     2941 gccgcacgcc acggccaggc acagcgtcgc ggcaaccaga accctgcgcc tcattcgccg
     3001 cctccgtcgc cgggcgagcg ctcgggcagt cgcaacgtga atgtggtgcg gccgttcccg
     3061 gaggtcacgg cgattgcccc gccgagcagc ctggcgttct cctgcgcgat cgcgagcccg
     3121 agcccgactc ctccgcgctc gcgcgagcgg ctgatgtcgg accggtagaa gcgcgtgaag
     3181 agcttctctt ggtcggccgc cggaacgccc ggtccggcgt ccgacaccga gatgaggtac
     3241 gcgccgtcgt cgcggccgat ctccacgcgg acatcgtgtc ctccgccgtg cgtgtaggcg
     3301 ttgtcgatga ggttgccgag cacccgctcc aggcgtgcct tatcggtctc gaccgtgtga
     3361 tcgtcgccgc tcaggcgcgc atcgtagcgg cgtcgatgca gcagcgcctc cgagaaggcg
     3421 cgcagccgca cgggctcgat gcggatgggg gctctgcccg cgtcgagctc ggagatctcg
     3481 aggagctccc gtaccaacag gttgagtgcg ccgatgtctt cgcgaatcag atcgagcgct
     3541 tcgcggcctt ccgccgagag gtgctcgcgg tgcgccgcga gcacgtccgc cgacgcttgg
     3601 agcgcggcga gcggcgtgcg aagctcgtgc gaggcggcgg cgacgaagta gcgttcgcgc
     3661 tcgatcatgc cgcgcaacgc ctccgccatg cggttgaacg acgtggcgag ctgtcccacc
     3721 tcgtcctggc tggtgggcgt gacgcgcgtc tcgagcaggc cctcggccat gcgcctggtc
     3781 gcgtcgctga ccgcggcgag cggatgcatg atgccgctcg tgacccgccg cgcgaccagc
     3841 gtgaccacca ccacgatcag cagaccggcg acggccatca cccggccgag cagcgagatc
     3901 gtgcgatcga cgtcctcgag cggaaagaac aggtacaggt ccatgcccgg cggcgggagc
     3961 gggctgccga agatcagcag ccggttcgag cctggcggct gcgcgatctg atacccgagc
     4021 cgttcgttcc tgacgatcgc gcgtagtccc gagggcacgg cgtccggcgt gagcgcaagg
     4081 ctcgtggcga accattcgcc gtggtcggtg accatcgcgt cgaagctctg ccggatctgc
     4141 agccgcgtca cgagctccgc ggcattgggc tccgcgcgca cgaactcgcg ggcgaacagc
     4201 agcgcgaaga ccgtctgccg gttggaggag cggacgcgct ggctctccat gaacgagcgg
     4261 accgtcagca gcgtcaccag cgacagcgcc gtgaccagga tcgccgcggt ggccacgaac
     4321 gcgatggtca cgcgcagccg caagctgcgg cgccggagca gcgcgagcgg ggatctcacg
     4381 gttcggacga cagcttgtag cccagccccc gcaccgtctg caccagcgcc ggatgcgccg
     4441 ggtcggactc gatcttggcg cgcaggcgcc gcacgtgcgt gtccactagg cgcgagtctc
     4501 cgagatagtc gtagccccag acctgctcga gcagaacctc tcgcgtgaag atcacgtcgg
     4561 ggcgggatgc gagcaagcag aggagcttga actcggtttt agtgagcggc acgcgctcgc
     4621 cggctttgag caccacgccg ctctcgggct tgatctcgag ggcgccgaag ctcatcttgc
     4681 gcattggcgc ctcggactgg acgcggcgca gcagcgcctt gatgcgagcg atgagctcgc
     4741 gagtctcgaa gggcttggtt acgtagtcgt ccccgccgga ctcgagaccg accaccacgt
     4801 ccacggtgtc cgtcttggcc gacaggaaga tcaccggcat gtcgctgctc ttgcgaatct
     4861 ccctgcacaa ctcgaagccg tcccggcggg ggagcatcac gtcgaggatg gccaggtccg
     4921 gtttgtcctt ctcgagcaga gcgagcgcct cgtcggcgtc ctcggcctca agcgtgcgca
     4981 ggccttcctg gcccagggcg agcgcgatgc tgcgccgaag gcggcgatcg tcgtccacca
     5041 gcaggatcgt ggcgtccaca gggcaatcat acggacgccc ggccgctgcg tgggaccgtc
     5101 accaaaccgt aacgaagccg tcggcattcg gccacacggg ccctgtaggt tcaccttcga
     5161 gccgaaacgg gcgggaggag cgagcgtgag tcagacctac gtactcgtcg aagtcgagcc
     5221 gggcgcgatc gatcgtgtcc tcgacgagct cgtcctgctt cccggagtgg ccgaagcgac
     5281 ctgcgtcgtc ggtggcttcg atctgatcat cagagcggag gtggccgacg aggcgctgag
     5341 cgcgtccctg cgcgcgcaca tcgcctcgat gcgtggtgtc acccgtgcgc tgccgtgcga
     5401 ggtgcgccgc gagccggtag gcgtcgcctc cgctctctaa gcttctcctg ctttaccagc
     5461 tgcgccacgc gcccgggccc cggaaggccc gggcgcggag gcgcccaagc cgccccgcta
     5521 tgctgactgg cgcgtgacgg gccccgttga gatccatcac ctgatcgccg ccggatcctc
     5581 gctcgccggc ggtctccttt tgggactctt catctaccgg ctggttcttc ggccgttggt
     5641 gaagcactcc acgctgacgc aggcgggcgc cgatgacgtg attctcccgg cgatccgcaa
     5701 ggcgacggtc ctgtggttcg ccgtcgcggg cgctttcgcg gcgcgcctgt ccctgccgct
     5761 tgcggcgggc ccggcgaggg t
//
LOCUS       DATLFO010000141         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_62304, whole genome shotgun sequence.
ACCESSION   DATLFO010000141 DATLFO010000000
VERSION     DATLFO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62304"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VM841_05385"
     CDS             <1..361
                     /locus_tag="VM841_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89651.1"
                     /translation="LTADLADGAAFDPASGDWTLMPESPLRARREAVSVWSGREMVVW
                     GGHEAAFAERQQGFADGASYDPVSRRWRRFGDDSLTARFTRGLWTSKGFFLWGGWSMD
                     DSPSDLADGAILSVGSS"
     gene            413..1390
                     /locus_tag="VM841_05390"
     CDS             413..1390
                     /locus_tag="VM841_05390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HVL89652.1"
                     /translation="MKAIVCKELGPPSSLVFEEVPDLEPGPGEVVIDVKAAGVNFPDV
                     LIVQGLYQFKPPLPFSPGHEVAGDVRSVGEGVTSLKPGDRVIAVVGHGGFAEQVAVEA
                     ARCFPMPDAMDYATGAGFVLAHSTSHYALRDRAKLQEGETLLVLGAAGGVGLAAVELG
                     LAMGARVIAAASSDEKLETCRKRGAAETINYETEDLRARVKELTGGVGVDVCYDPVGG
                     KYAEPAVRSMAWDGRYLVVGFAAGEIPKIPLNLLLLKSCNLQGVFWGAFTARDPKRFA
                     ELMRELFGWWEQGKIAPLVSETYPLDRAVDALADVANRKVRGKAVLLVE"
     gene            1514..2317
                     /locus_tag="VM841_05395"
     CDS             1514..2317
                     /locus_tag="VM841_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HVL89653.1"
                     /translation="MLRRVRLIAAALAAVALFVAPLPAPAAPAWASVATATIYPGVST
                     ISDGNQCTANFVFFDETSIYLGLAAHCISIDDATSTDGCLARIQPGVREVQIQGATKR
                     GQMVYNSWSTMQQRRETDRTTCRYNDFALVRLDPADHGRVNPSVPVFGGPSSGTAATS
                     TGQNVYGYGNSWLWLGVPAARPHRGVTLSSSAWVHKVYTVAPGIPGDSGSAYLDAGGR
                     ALGVLSALELAPFAGSNDVIDLGRALTYMRNNTSFKTVQLATGTQPFRP"
     gene            2479..>2842
                     /locus_tag="VM841_05400"
     CDS             2479..>2842
                     /locus_tag="VM841_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89654.1"
                     /translation="MRILVAEDEPAVRSLIEQVLALEGFEVKACANGGEAMAALHQFK
                     PHLAVLDVMMPVSDGIGVLRAIREGDAPDLPVILLTARADDQSTWNGWRAGCNLYLTK
                     PFDPEDLVSAIRQTIASLR"
BASE COUNT          438 a          963 c          977 g          464 t
ORIGIN      
        1 gctaacggcc gatctcgccg acggggccgc gttcgatccc gcatccggcg actggacgct
       61 gatgcccgaa tcgcctttga gggcgcgacg cgaggccgtg tcggtttgga gcggtcgaga
      121 gatggtggtg tggggtggcc atgaggcagc cttcgcagag cgacagcaag gatttgccga
      181 cggcgcttcc tacgatccgg tttcgcggcg ctggcgacgg ttcggcgatg attcgctgac
      241 cgcgcggttc acccgaggtc tctggaccag caaggggttc tttctctggg gaggctggtc
      301 gatggacgac tcgccgtctg atcttgccga cggggccata ctgtccgtcg gcagctcgtg
      361 agcgacagcg cgcatcgcgc aactcccccc gacttgcgcg acaatgcccc gcatgaaagc
      421 catcgtgtgc aaagagctcg gaccgccgtc gtcgctggtc ttcgaagaag tgcccgatct
      481 cgagccggga ccgggcgagg tcgtgatcga cgtcaaagcc gcgggcgtga acttcccgga
      541 cgtgctgatc gtgcagggcc tgtaccagtt caaaccgccg ctgccgttca gccccggcca
      601 cgaggtcgcg ggtgacgtgc gctcggtcgg cgagggcgtg acgagcctca agccgggcga
      661 ccgagtgatc gccgtcgtag gacacggcgg gttcgccgag caggtcgcgg tcgaggccgc
      721 tcgctgcttc ccgatgcccg acgcgatgga ctacgcgacc ggcgccggct tcgtgctcgc
      781 tcactcgacg tcgcattacg cgctgcgcga tcgcgcgaag ctgcaagagg gcgagacgct
      841 gctggtgctc ggcgcggccg gaggcgtcgg cttggcggcc gtcgagctcg gcctcgcgat
      901 gggtgctcgc gtgatcgcgg cggcctccag cgacgagaag ctcgagacct gccgcaagcg
      961 cggcgcggcc gagacgatca actacgaaac cgaagacctg cgcgcccgcg tgaaggagct
     1021 caccggcggc gtcggcgtgg acgtgtgcta cgacccggtc ggcggaaagt acgcggagcc
     1081 cgcggtgcgc agcatggcct gggacggccg ctatctggtc gtcggattcg ctgccggcga
     1141 aatcccgaag atcccgctga acttgctgct gctgaagtcg tgcaacctgc aaggcgtctt
     1201 ctggggcgca ttcaccgcgc gcgatccgaa gcgattcgcc gagctgatgc gcgaattgtt
     1261 cgggtggtgg gaacagggca agatcgcgcc gctcgtgtcg gagacctatc cgctggaccg
     1321 cgcggtcgat gcgctcgccg acgtggcgaa ccgcaaggtg cgcggcaagg ccgtcctgct
     1381 cgtcgagtag cggcgcaacc ggccgcggag cgcgggatcg ggccggccgg cacccggctt
     1441 tgagtcgaac agcgccggga tgccccggtt tgtcgggggt ccggacccgg cgcggcttcg
     1501 gtaccctgga cgcatgctcc gtcgcgtccg tctgattgct gccgccctgg cggcggttgc
     1561 gctgttcgta gcgcccctgc ccgcccccgc ggccccggct tgggcctccg tcgccacagc
     1621 caccatctac ccgggcgtga gcacgatcag cgacggcaac cagtgcaccg cgaatttcgt
     1681 cttcttcgac gagacctcga tctacctcgg cctggccgcc cactgcatca gcatcgatga
     1741 tgcgacctcg accgacggct gcctggcgcg gatccagccc ggcgtgcgcg aggtccagat
     1801 ccaaggagcg acgaagcgtg gccagatggt ctacaactcg tggtccacga tgcagcagcg
     1861 gcgtgagacc gaccggacga cctgccggta caacgacttc gcgctcgtgc gtctcgatcc
     1921 cgccgatcac ggccgggtca atcccagcgt gccggtgttc ggtggaccca gctccggcac
     1981 cgccgcgacc tcgaccggcc agaacgtcta cggctacggc aactcgtggc tgtggctcgg
     2041 agtgccggcg gctcgcccgc atcgcggcgt gacgctcagc tcgagcgcat gggtgcacaa
     2101 ggtctacacc gtcgcccccg gcattcccgg cgactccggc agcgcgtatc tcgatgcggg
     2161 cggccgcgcg ctcggcgttc ttagcgccct cgagctggca ccgttcgcgg gcagcaacga
     2221 cgtaatcgac ctgggtcgcg cgctgacgta catgcggaat aacacctcgt tcaagaccgt
     2281 gcagcttgcc accggaactc agccgttccg cccgtagcgc ccgcccgata tcggcgcgtt
     2341 ccgcacggtc cgtccccggc ctgtctagga aacgtctagt catcccggcc cgaaaggacc
     2401 acccgcactg ggctcaggat tcgggaccga cgtaccgatt ctttcatcgt ccgggagtac
     2461 gagcggaggg ccgaaacgat gcggattctg gttgccgaag acgagcctgc cgtgcgctcg
     2521 ctgatcgagc aggtgctggc gctcgaggga ttcgaggtca aggcatgcgc caacggcggc
     2581 gaggccatgg ccgcgcttca tcagttcaag ccgcacctcg cggtgctcga cgtgatgatg
     2641 cccgtcagtg acggcatcgg cgtgcttcgc gcgatccgcg aaggcgatgc cccggacctt
     2701 cccgtgatcc tgctgaccgc cagggccgac gaccagtcca cgtggaacgg ctggcgcgcg
     2761 ggctgcaacc tgtacctgac gaagcccttc gaccccgagg atctcgtctc ggccatccgc
     2821 cagacgatcg ccagtttgcg gg
//
LOCUS       DATLFO010000142        19949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_12742, whole genome shotgun sequence.
ACCESSION   DATLFO010000142 DATLFO010000000
VERSION     DATLFO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19949
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12742"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VM841_05405"
     CDS             complement(<1..420)
                     /locus_tag="VM841_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89655.1"
                     /translation="MTSRRGIAAALLLSLVATACGSARASGVFRASDVDGGVEVRTGP
                     GGDWEPLINGRSVDVLDEVRTGPGASVKLGSDRTRLELGADTWIVVGSLAVATAKTGS
                     VLVETEQPVRVSDGGGVIVIGPGTFRLDYGRRVGAYRG"
     gene            complement(417..3278)
                     /locus_tag="VM841_05410"
     CDS             complement(417..3278)
                     /locus_tag="VM841_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89656.1"
                     /translation="MEGQLVVPFHFAAEFLILAVSVGACLDAVRRARSGGGARALSQA
                     AGFALLAVAQVVHGARIPGAAEDGAAIPLLLRAAGFALVAAGGVTLFAPRRDQAAPVL
                     GIAPVLAAGLFAAGDASRLALVPALAAAAAMVRGAAAHRGDQDPASFAFAAAFASFAG
                     GEVALAFAPADGGTLLAVSHALRFAGALLLVRWLGVTIARSVRLRFVTAFVVALTALV
                     LVLAAAFDVVIARNVEREELRRVGVTAAALSGSIDRQVRSDQQTAFNLGQAAQATVPG
                     RSGLSALACLYPDLDILLSVDAAGRIVGSALKPPRRCDFSNLGAYVAKTPSPEVPQAL
                     RLGVAGSLVVVDNALAGRSNAGLEVISPGTLAIVAAAPIGPVARPAGAIAIARIVDAA
                     YLGRLRAGADDLALLVGGAVVAFTGTQDAAAGLQQALDGPAGREVRRDAEEDGDTLGL
                     SVPLGGVRSFASFVPLKRATGEPVAVLAAVSSAGDVLASRESLNRVLFLIALAAAFIA
                     AAIAALAGGHVTRPIRGLRVAAEAIRRGDLSARAPASAPDELGDLGRSFNEMAVSLDR
                     STADLRAAAITEAELRERMEAIMQSMGDGLVATDAAGRIVTVNRAAETITGLTAAKMT
                     GKRLDAVVRGTAGEQAIADAVAGPAGSFSGVLERGSERVPVAITAAPLRDQSGSEAGR
                     VVVLRDVSAEVQAERMKSEFLSNVSHELRTPLTPIKGYTEILKRKKFSKDKAETFLDG
                     ILESTARLERIVEILVDFAAMEAGRLTARTEAVPVKSFVQDAVSRWKDRPGARFTVKV
                     PEGVPSVNADPRLLAKSLDELIDNAVKFSPDGPRVTIEAVPHSNGSRSRKPSGVKIIV
                     RDAGIGIAKDQLPTLFRDFRQIDGSETREYGGLGLGLAYVKRIAAVHGGEVTAQSTPG
                     KGSVFTLLLPAADTERKGKAARTPRAAATSAAKKKAGKQ"
     gene            complement(3370..4425)
                     /locus_tag="VM841_05415"
     CDS             complement(3370..4425)
                     /locus_tag="VM841_05415"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVL89657.1"
                     /translation="MESRPEFPHPISIRSRIPGWWRATLASTLLIPALVAGGLLYHTD
                     FFALSPGPATDVSGLIRVEGDKRDSRGAFLITTVSVTIDTINVFDYLRASIDPAIEAL
                     PRSVLVGPGLTDEQQDEVNQADMEQSKYAATVVAMRAAGLNVAPIAGARVIKVFSGTP
                     AEKALRDGDVIVSVGGLAVGSVSEMSEAIRAHPVGTKLDFGIIRGGKAMKVAITTTAD
                     SSDPSRPIIGIRPDEAFRLPVNVTIDSQDIVGPSGGLVFALAVADALTPGDLTRGHKI
                     AATGTIALDGTVGRIGGVEQKVRAAEHVGADVFLVPFDEQAQAKAVARSIKVFGVRDI
                     ADAVAVLDGLQPIKRAA"
     gene            4586..4930
                     /locus_tag="VM841_05420"
     CDS             4586..4930
                     /locus_tag="VM841_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89658.1"
                     /translation="MTDSAGLTVTLLITGAILAFGASYAVNAAMPPRLRRDRTAHAPK
                     ARPKPEPAPLTGGATGLLESIPFLESAEKDKRLHGAGRLVVGIVAAAAVLALSLFLFA
                     SRLTAWLDAVGF"
     gene            complement(4973..6247)
                     /locus_tag="VM841_05425"
     CDS             complement(4973..6247)
                     /locus_tag="VM841_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89659.1"
                     /translation="MKRLLAAAAVVAMSASAAPAAPDPDDPGFSQQWGLKTMRVPQAW
                     EASRGDGVIVAVVDTGVDAAHPDLKGRVLPGRDFVDDDADAADEKGHGTHVAGTIAAT
                     TGNGVGIASVSPGVKILPVRVLGADGEGDPGDVAGGIAWAVEQGARVINLSLAQDDHL
                     PVAGDALLRDTRVDRAIRKAAEDGAVVVVAAGNDDSGGKPQTAYDATVPGVLVVGATT
                     RGDERAAYSNYGDGLDVVAPGGGTAADPKACADPNWIVSTWWNPQTRKSDYGGGCGTS
                     MAVAHVSGVASLLLARGYSSAGAAKRIVDTAKDIGAPGRDVQTGNGRVDAMAAVGPLT
                     ATPRPTRSASSPTAAPSATTQGGLSGAGTPTATPTPTTIEPGPASPPQAAPPAEPAGR
                     GGPITMAVVLMLAVMAAQSRARARAGAGPPAG"
     gene            complement(6244..7260)
                     /locus_tag="VM841_05430"
     CDS             complement(6244..7260)
                     /locus_tag="VM841_05430"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVL89660.1"
                     /translation="MPVIRDIALDLGTATTLAWLPERGIVLREPTVVALERTNARCVA
                     VGRESWTAAAASPETVTATWPFRRGPASDFAITERIIEHVLAKAGSAKITRPRMLLVV
                     PTGATEVERRAMEEAALSVGAREVWLIEAPLAVALGANLPIAEPRGSCVVDIGGAATE
                     VAVISMGGMVSSQHVAVGGFDADDLIVKFLRREYGMVIGERTAEGIKIQVGSASPMEE
                     ELKAEIRGREIETGAPKTVIVTSEEIRSALEESVTPVIEGLRSVLSRTEPELSHDVLD
                     GGIALAGGGGMLRGVADRIQSETGIPVRLVDKAADVAALGAGIALSDLEAMHHAGVAM
                     RRSS"
     gene            complement(7426..8454)
                     /locus_tag="VM841_05435"
     CDS             complement(7426..8454)
                     /locus_tag="VM841_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL89661.1"
                     /translation="MTTIAITGVGGGLGRCLLARLDADPTVERVVGIDAVPPRDGSPK
                     MEFFESDVRDERIGRFLDGCDAVVHLAFAPDSARRPDEMRSVNVDGTRNVAAAARAVA
                     GGRLVFASTATVYGANPDNDFPLTERSPVRPSADYAYAVNALDAEEAAAEAGDAVVLR
                     LANVFGSDTDNFVMRMFQSPRFLTIRGHAPPLQMVHQDDAAAALHLACGRALSAGIYN
                     VAADGWMDLSEALALAGRRSVEMPEAVAYSLAERAWRLNLSGAPPAEIAYLMYPWVMD
                     NAKMRAAGWTPARTNRETLVEAVEAHRPWVALGRGKVRKADLAKGAAATVGLLAAMGA
                     LRRSRRRP"
     gene            8522..9721
                     /locus_tag="VM841_05440"
     CDS             8522..9721
                     /locus_tag="VM841_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HVL89662.1"
                     /translation="MSTDPSGLPPDLPPDILGRIPLFAELQKVLSWAGGPVNWDLARQ
                     VAVAAAAAAGPQTVDPAVAAEIGEDARIAHLWLAETTGIGEAGVVPPAKAVSPADWAE
                     HACTAYREMIDPLAAKVSAAIGEQAGSALGEDAGALPPGLDPGAIGAALRQMAPLFLG
                     VQTGGVLGAVAQDVLGSRDLPIPPADGAPVIVVPSAIAQLADEYQLDRREALLYVVLH
                     ECAHRAIEESLSTFRSSFFALFHDYVASLSIDFSSAFERLQEIDFSNPQGVEEQLGGQ
                     GFFDLLDSPASSAAAGRLEHLIALAEAFADRMVEAAAEGRLPSAARLAEAAARHRSGA
                     EGTAALHKFMGVGASDEARRAAARFIRAVLSEAGWPALIRMWEDSETMPTKAEIDDHA
                     AWLGRIR"
     gene            9718..10413
                     /locus_tag="VM841_05445"
     CDS             9718..10413
                     /locus_tag="VM841_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVL89663.1"
                     /translation="MTALHVGCGGFPVEPAEYATQLSYVEIQETFHEGPELDAVKKDL
                     ERVAGDLRIGLVASKVITHPRKDGVYKKPGPDVPDHASVGHFTRSRWTDEAWERMDVL
                     VRALKAAVVVLRTPPSFKKTPANALALENFIAHAERPGLEIVWEHGGTWPETDAAEVA
                     HRLGLVIASDLTGTIPDGEVYARFTGGAKGTSALTDAAGAKAAQKLTGRNGFVVFANK
                     SCWEDARRFSKTV"
     gene            complement(10430..10987)
                     /locus_tag="VM841_05450"
     CDS             complement(10430..10987)
                     /locus_tag="VM841_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89664.1"
                     /translation="MHTRLLRRLLRRPLRPLLSLGVLLALVACSGGPSKDPRFTGAAG
                     EPAKTSVSPEAEPRQGSGEVELPGNLPPAAMFLGSGSHRTTPARYCVGEDCALLKPPA
                     PPALEGPAESPVLFTLSAAPLRARLEVSAAGKAPEIVALTPGSSMSWQPAMPPGQYRL
                     ALTAIYDTTEVSWPFALKIVEGTRR"
     gene            complement(11035..11529)
                     /locus_tag="VM841_05455"
     CDS             complement(11035..11529)
                     /locus_tag="VM841_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL89665.1"
                     /translation="MRFKVEVVRSPRRRKTVEAVKDGDRVTVMLPATLTRAEARRWVK
                     TMVDRIEESEQIQRLNEKRDLQRRAAELHRRYFYGRPKLNSIRYVANMKGRYGSCTPA
                     DGTIRLSHKLSDCPDWVRDYVIVHELAHLRIPDHGPSFWRLVNRYPLAERARGFLISK
                     GLDE"
     gene            complement(11754..12233)
                     /locus_tag="VM841_05460"
     CDS             complement(11754..12233)
                     /locus_tag="VM841_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL89666.1"
                     /translation="MLDAQLDHVAIAVRDIRAAMSLYCDALGATFLFAGDQHDQGFRF
                     AQFRFPFGGKIELVAPLGDGFVQRFLDARGEGVHHVTLKTPDIERALEHLRSTGVEPF
                     GVNIAGSQWKEAFIHPKHANGTLIQIAQAAWSDEEMARHHLADHSQAGHRHIGIEDL"
     gene            complement(12546..13334)
                     /locus_tag="VM841_05465"
     CDS             complement(12546..13334)
                     /locus_tag="VM841_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL89667.1"
                     /translation="MADAPVLVERSGGIVTLTLNKPDVRNAMTADLTDAFTAAIDDLR
                     EDQDARVLIITGAGKAFCAGGDLSFIQPGPGASVPAIRAKMRAFYGKFLAVRDLEIPT
                     IAAINGSAVGAGLCLALACDLRLAAADAKLTAAFTKLGLHPGMAGTYLLTRLVGSARA
                     ADLLFTSRVVSGAEAESMGLVNRAVPRESLAAETRALAEEIAANAPVATRLVKRAIQL
                     AERNDLDTMLEYEALAQPVTMATADLLEGLAAVKEKRAPSFDGT"
     gene            13413..13745
                     /locus_tag="VM841_05470"
     CDS             13413..13745
                     /locus_tag="VM841_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89668.1"
                     /translation="MYEFAIVALLGLALYKTVDFLTGLTKMDEPAIRTMATLGLGVVA
                     TWILDYSIFAAWGVTIREAWMGPVFSGLIVGGFAYIWHDALGALEGYGRRARDEALEI
                     ENRIPRAA"
     gene            complement(13819..15261)
                     /locus_tag="VM841_05475"
     CDS             complement(13819..15261)
                     /locus_tag="VM841_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVL89669.1"
                     /translation="MSDRLDDAIAILTGDECDGIVDLVLCMRNGIVEAHARDGMVAFT
                     AQGPLHMNGRNPLEHGDPGAMSPLEAERANVRPSNRANHYPFAYDNIAHLFDHPHAPD
                     IAVVHTPAHNWEERGGHRGEHGSPNLIQSRAPLIAAGRGIRRLGSIPRAARMVDIAPT
                     LTLLAGGRPRPDGPLIARQHGRVLEEILDLDDPPKRVVAFLCDGTNANVLYAMARAGE
                     LPNTAKLMDAGTVYEHGLIASFPTVTLANHTTALTGVHPGVHGVLHNAYYERATGRAV
                     NTNSPATWHLARNEIASDVETVYEALARSGAGFTASINEPCDRGASYATFDAVRDGGE
                     VVQRLQTALPDPTAIPGATQTFVRTKPEYAWSTAADYMAVQQAGALWRGDAGNPRPRF
                     MWVNLILNDAGNHAGGPYSDIGHAALRDTDARLGEIMDAIGDDDTAYVVLADHGMEES
                     DPECTGDYSEALEKAGIAFRDEGFGFLYLG"
     gene            15288..18059
                     /locus_tag="VM841_05480"
     CDS             15288..18059
                     /locus_tag="VM841_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HVL89670.1"
                     /translation="MPARTILHVLPHTHWDREWYEPFESYRFRLVRTVDALLDLLDVR
                     PGFHFHFDGQTAAIEDYLAVRPEAAPRVAAHVAAGRLLVGPFRILMDEFLCSPETIVR
                     NLRQGLATATRLGVRPRIGYIPDSFGHVAQMPQILRLAGMQDALVWRGVPAAIDRPAF
                     TWVAPDGTEIRTLYMATSYSNAATLPETFEDLMVRAKRIVADMEPFRPEGVVLAMNGS
                     DHLAPSATLPAALDQANERQDEIEFRIAPLATYAADAPRENLPRWHGEMRSSARANVL
                     MGVLSARMPLKQAELAASRMLERYAEPLAAIAGFDPGGMLDEAWLGMVENSAHDSVCG
                     CGIDAVAFAVASRYERAERIGAGVRDTALQMLAERAAVAAPDGRPDTEGVLLWNPSPF
                     SRAGVCEAVMALPGHGGDAELAGPDGSRHPVQRLAETEQVVVDMVLRGAELARIVPTI
                     HSRMMGTLSVNAMTLTDGRVRTVRLELGPIPVGHFDVEAAKREVEEAAKRSPRAKFHV
                     IGNGPPLARLLVDAPETGGLGWTTLFPVAAEAPAPTRVSAESSTISNEHVRVTLDEQG
                     RATVEAAGMRLDGIGLVDGGEAGDEYTYSPPARDTVVSEPRSCTNEIVSTGPLEARIR
                     SWQTWRVPAALDPSRTRRARRTVEMPVVVEWAVRAGEPFARVDVRVDNTAADHRLRLH
                     VPLPGRVEGSHADTAFHVTARGLDGESGYEHATPTFPSRRWVDTSDGARGVAVLHTGT
                     PEYEITGGTQIAVTLLRCVGWLSRQDLTTRSGPAGPMLAAPGAQLIGPHTFSLAVYPH
                     EGDWHAGNVPRVAETFGHPFAALALRPHDGDLPPSGSALRVEPASVQLSALERRGGAW
                     EARIYNASDAACEARLSAGAPLAAHEAWTVDLTGERATPLPCDAGTISLVLRPWQIET
                     VRFVPREAT"
     gene            18081..19169
                     /locus_tag="VM841_05485"
     CDS             18081..19169
                     /locus_tag="VM841_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVL89671.1"
                     /translation="MTLPRVLVLAVALSAAASSCSAGPPPPERTRTVIVHAAKALPSS
                     LPSTLEEMQGVKWIRVATTAMVNLVKVEGGKLLQPRAPSSVLPFSIGAAEPLPADGDA
                     VSAWLAQGTAVLSTASARLRGLRPGGAITVGLGDKRERIVIGGVRDDARTSGVEALVP
                     LAVASRLGVTEVRQVVVEVTESGSAPLQDTARMLAAPVPVRVGSREDVATPDAGKLLS
                     LAEIKERFGEFSFVDVPGRWLEPDPAWEEANIATQHVPILGRMRCHKKVMGPLTAAML
                     EVQAAGLEHLVVEPSFCYAPRVQFGDARSISRHTWGIAVDINPTGNAFGDEPTQDPRL
                     VAIMAKHGFSWGGVWMTPDGMHFEYTGL"
     gene            19230..19724
                     /locus_tag="VM841_05490"
     CDS             19230..19724
                     /locus_tag="VM841_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL89672.1"
                     /translation="MVGVSREGLSEPVSGRLAGLYALYGHDLKRLAFLMTGDASLAED
                     LMHDAFLRMAGRLGHLRQEDRALAYLRKTVINLVRDRGRRRDQTPLPDPPHERDLDAE
                     QDLRDLLMTIPARQRAALVLRYYLDLPDDEAADVLRCRPATVRSLVARGLSALRINSE
                     DDHG"
     gene            19717..>19949
                     /locus_tag="VM841_05495"
     CDS             19717..>19949
                     /locus_tag="VM841_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89673.1"
                     /translation="MDERLFRERLARAAGRVPPPATDLQDLRRRGHRRAARTIVAALA
                     AVALVTAGVSTVALTRTARRAEPVTPPAATGPC"
BASE COUNT         2786 a         7119 c         7173 g         2871 t
ORIGIN      
        1 gccgcggtaa gcgccgacgc gccggccgta gtcgagccgg aaggttccgg gcccgatgac
       61 gatcacgccg ccgccgtcgc tcacgcgcac gggctgctcg gtctcgacga gcaccgatcc
      121 ggtcttggcg gtcgcgaccg cgagcgagcc gaccacgatc caggtgtcgg cgccgagctc
      181 gagccgggtt ctgtcgctgc cgagcttgac cgaggcgccc ggacccgtgc ggacctcgtc
      241 gaggacgtct acgctccggc cgttgatcag cggctcccag tcgccgccgg ggccggtgcg
      301 cacctcgacg ccgccgtcga cgtcgctcgc ccggaacacg cccgacgcgc gggccgagcc
      361 gcatgccgtg gcgaccagcg acagcagcag cgctgcggcg atccctcgac gactcgtcac
      421 tgcttccccg ccttcttctt cgccgcagac gtggccgcgg ccctcggcgt gcgagcggcc
      481 tttcccttcc gttcggtatc ggcagcgggc agcagcaacg tgaacaccga gcccttgcca
      541 ggggtgcttt gcgcggttac ctccccgccg tgcaccgccg cgatgcgctt gacgtaggcg
      601 agcccgagcc cgaggccgcc gtattcgcgg gtctcggatc cgtcgatctg ccggaagtcg
      661 cggaacagcg tgggcagctg gtccttagcg atcccgatgc cggcgtcgcg cacgatgatc
      721 ttgacgcccg agggcttcct ggaccgcgag ccgttcgaat gcggcaccgc ctcgatcgtg
      781 acgcgcggcc cgtcgggcga gaacttcacg gcgttgtcga tcagctcgtc gaggctctta
      841 gcgagcaggc gcggatcggc gttgaccgac ggcacgccct cggggacctt gacggtgaag
      901 cgcgcgcccg ggcggtcctt ccagcgcgag acggcgtcct gcacgaacga cttgaccggg
      961 acggcttccg tgcgcgcggt caggcggccg gcttccatcg ccgcgaagtc caccaggatt
     1021 tcgacgatgc gctcgagccg cgcggtggac tcaaggatgc cgtcgaggaa cgtctcggcc
     1081 ttgtccttgg agaacttctt gcgtttgagg atctcggtgt agcccttgat cggcgtgagc
     1141 ggggtccgaa gctcgtgcga gacgttggac aggaactcgc tcttcatgcg ctcggcttgc
     1201 acctcggccg acacgtcgcg caggaccacc acgcggccgg cttcgctgcc cgactggtcg
     1261 cgcagcggcg ccgcggtgat cgcaaccggc acccgctcgc tgccgcgctc gagcacgccg
     1321 ctgaacgagc cggccggtcc ggcgacggcg tcggcgatgg cctgctcgcc ggcggtgccg
     1381 cgcacgaccg cgtcgagccg cttgccggtc atcttcgcgg cggtcaagcc ggtgatcgtc
     1441 tcggcggcgc ggttgacggt cacgatccgt ccggccgcgt cggtggcgac cagcccgtcg
     1501 cccatcgact gcatgatcgc ctccatgcgc tcgcgaagct ccgcctcggt gatcgcggcg
     1561 gcgcgcaggt ccgcggtcga tcggtccagc gacacggcca tctcgttgaa gctgcggccg
     1621 aggtcgccga gctcgtcggg cgcggatgcc ggcgcgcgcg ccgacagatc cccgcgacgg
     1681 atcgcttcgg ccgcgacgcg cagaccgcgg atcggccggg tcacgtgccc gccggccagc
     1741 gcggcgatcg cggcggcgat gaaggcggcg gcgagcgcga tcaggaacag cacgcggttg
     1801 agcgactcgc gcgaggcgag cacgtcgccg gcgctgctga ccgcggcgag caccgcgacc
     1861 ggctcgccgg tcgcgcgctt gagcggaacg aaagacgcga acgagcgcac gccgccgagc
     1921 gggacgctca ggccgagcgt gtcgccgtcc tcttcggcgt cgcggcgcac ctcacggccc
     1981 gcgggtccgt cgagcgcctg ctgcagaccg gcggccgcgt cctgggtgcc ggtgaaggcg
     2041 acgaccgcgc cgccgacgag cagggccagg tcgtcggcgc cggcgcggag ccggccgaga
     2101 taggccgcgt cgacgatccg cgcgatcgcg atcgcgccgg ccggacgcgc gaccgggccg
     2161 atcggcgcgg ccgccacgat cgcgagcgtg ccggggctga tgacctcgag gccggcgttg
     2221 ctgcggccgg cgagcgcgtt gtccaccacg accagcgatc cggccacgcc cagccgcagc
     2281 gcctgcggca cctccggcga gggagtcttg gccacgtagg cgcccaagtt cgagaagtcg
     2341 cagcggcgcg gcggcttgag cgccgagccc acgatgcgcc cggccgcgtc caccgacagc
     2401 aggatgtcca ggtccggata caggcacgcg agcgccgaca ggccggaccg gccgggcacc
     2461 gtcgcctgcg cagcctgccc caagttgaat gcggtctgct ggtccgagcg aacctgccgg
     2521 tcgatcgatc cgctcagcgc cgcggcggtc accccgacgc gccgcagctc ttcccgctcg
     2581 acgttgcgcg cgatcacgac gtcgaaggcg gcggcgagca cgagcacgag cgcggtgagc
     2641 gcgacgacga aggcggtgac gaaccgaagc cggacgctgc gggcgatcgt cacgcccagc
     2701 cagcggacca gcagcagcgc gccggcgaag cgcagcgcgt gcgagacggc gagcagcgtc
     2761 ccgccgtccg cgggcgcgaa ggccagcgcg acctcgccgc cggcgaagga cgcgaacgcc
     2821 gcggcgaacg cgaacgaggc gggatcctga tcgccgcgat gcgcggccgc gccgcggacc
     2881 atcgcggcgg cggcggccag cgccggaacc agcgcgagcc gcgatgcgtc gccggcggcg
     2941 aagagcccgg cggccaggac cggagcgatc cccaggaccg gcgcggcttg atcgcggcga
     3001 ggcgcgaaga gcgtgacacc tccggcggcg accagcgcga agccggctgc ccgaagcagc
     3061 aggggaatcg cggcgccgtc ctcggcagcg ccgggaatgc gggcgccgtg caccacttgc
     3121 gcgacggcca gcagcgcgaa gcctgccgcc tgcgaaaggg cccgagcacc cccgcccgac
     3181 cgtgcgcgcc ggacggcgtc caggcacgcg ccgaccgaaa cagcaaggat caggaactcc
     3241 gccgcgaagt ggaagggcac gacgagctga ccttccatga tggggagtat atcggcgcaa
     3301 aaatgcccga aaacaggggc tgcgccggga ctaaaccgtc tttggtcctt tctgactagt
     3361 cagaaaggac tacgccgcgc gcttgatggg ctgaaggccg tcgagcaccg cgaccgcgtc
     3421 ggcgatgtcc cgcacgccga acaccttgat cgagcgggcg accgccttgg cttgagcctg
     3481 ctcgtcgaac ggcaccagga acacgtcggc gccgacgtgc tcggcagcgc gcaccttctg
     3541 ctcgacgccc ccgatccggc ccaccgtccc gtcgagcgcg atcgtgcccg tcgccgcgat
     3601 cttgtgcccc cgggtcaggt cgccgggcgt gagcgcgtcg gcgacggcga gcgcgaacac
     3661 cagcccgccg ctggggccga cgatgtcctg cgagtcgatg gtcacgttga ccggaaggcg
     3721 gaacgcctcg tcgggccgaa tgccgatgat cggccggctc gggtccgagg agtcggccgt
     3781 cgtggtgatc gcgaccttca tggctttgcc gccgcggatg atgccgaagt ccagcttcgt
     3841 gccgaccggg tgcgcccgga tcgcctcgct catctcgctg acgcttccca cggccaggcc
     3901 gccgaccgac acgatcacgt ccccgtcgcg aagggccttt tccgccggcg ttcccgagaa
     3961 gaccttgata acgcgcgccc cggcgatcgg cgcgacgttg agcccggccg cgcgcatcgc
     4021 cacgaccgtg gcggcgtact tggactgttc catgtcggcc tggttcacct cgtcctgctg
     4081 ctcgtccgtc aggcccgggc ctacgagcac cgaacgcggc agcgcttcga tggcgggatc
     4141 gatcgaggcg cgcaggtagt cgaagacgtt gatggtgtcg atcgtgaccg acacggtcgt
     4201 gatcaggaac gcgccgcgcg agtcgcgctt gtcgccctcg acccggatca gcccggatac
     4261 gtcggtggcc ggaccgggcg agagcgcgaa gaagtcggtg tggtagagca gcccgccggc
     4321 gaccagcgcc gggatgagca gcgtggacgc gagcgtggcg cgccaccacc cgggaatccg
     4381 gctcctgatc gagatcgggt ggggaaactc gggccggctc tccatgaggg ccgaagcgta
     4441 cccgagggtc cggggcgccg cccggcatgc ggcggccctt ccggggcatc acggccgatc
     4501 cggcagccct gccgggtccg ggctggattc acgcgcgccg tcatggaaag atgagaggtc
     4561 gggaatttcg gtcccgctct ctcctatgac tgattcggcc ggtctgaccg tcacgctcct
     4621 gatcacaggg gcgatcttgg cgttcggtgc gagctacgcc gtcaacgccg cgatgccgcc
     4681 gaggctgcgc cgcgaccgca cggcccacgc ccccaaggca cggcccaagc cggagcccgc
     4741 gcccctgacc ggcggcgcca ccggcctgct ggagtccatc ccgtttctag agtcggccga
     4801 gaaggacaag cggctgcacg gcgcgggccg gctcgtggtg ggcatcgtcg cggccgcagc
     4861 ggtcctcgcg ctgtcgctgt tcctgttcgc gagccggctc accgcctggc tcgacgccgt
     4921 cggcttctaa cgcgccggct tgttccgccg cgcgcatcca acccgcggac ggctacccgg
     4981 cgggcggccc ggccccggcg cgcgcgcggg ccctggactg cgccgccata accgcgagca
     5041 tcagcacgac cgccatcgtg atcggaccac cgcgcccggc aggctcggcg ggcggggccg
     5101 cctgcggcgg cgaggcggga cccggctcga tcgtggtggg cgtcggcgtc gcggtgggcg
     5161 tgccggcgcc cgacaggccc ccttgggtcg tggccgaggg agcggccgtg ggcgacgagg
     5221 ccgagcgcgt gggccgcggc gtggcggtga gcggcccgac ggccgccatc gcgtccacgc
     5281 ggccgttgcc ggtctgcacg tcgcgtccgg gcgcgccgat gtccttggcc gtgtccacga
     5341 tccgcttcgc cgcgccggcg cttgagtagc cgcgcgccag cagcagcgac gccacgccgg
     5401 acacgtgcgc gaccgccatc gaggtcccgc atccgccgcc gtagtcgctc ttgcgggtct
     5461 gcgggttcca ccatgtggat acgatccagt tcggatcggc gcaggccttc gggtcggcgg
     5521 cggtcccgcc gccgggcgcg acgacgtcca agccgtcgcc gtagttcgag tatgcggcgc
     5581 gctcgtcgcc gcgcgtcgtg gcgcccacga ccagcacgcc gggcacggtc gcgtcgtaag
     5641 cggtctgcgg cttgccaccc gagtcgtcgt tgcccgcggc cacgaccacc accgcgccgt
     5701 cctcggcggc cttgcggatc gcgcggtcga cgcgcgtgtc gcgcagcagc gcatcaccgg
     5761 cgaccggcag atggtcgtcc tgggcaaggg agaggttgat cacgcgcgcg ccctgctcga
     5821 cggcccaggc gatgccgccg gccacgtcgc ccggatcgcc ttcgccgtcg gcgccgagca
     5881 cccgcaccgg caagatcttg acgccgggcg agaccgaggc gatgccgacc ccgttgccgg
     5941 tggtcgcggc gatcgtgccg gcgacgtgcg tgccgtggcc cttctcgtcg gcggcgtcgg
     6001 cgtcgtcgtc cacgaagtcg cgtcccggca gcacccgtcc cttcaggtcg ggatgcgccg
     6061 cgtccacgcc ggtgtccacg accgcgacga tgacgccgtc gccccggctc gcctcccatg
     6121 cttgcggcac gcgcatcgtc ttgaggcccc actgttgcga gaagccggga tcgtcgggat
     6181 ccggcgccgc cggcgcggcc gacgccgaca tcgcgacgac cgcggccgcg gccagcagcc
     6241 gcttcacgac gacctccgca tcgcgacgcc cgcgtgatgc atcgcttcga ggtcgctgag
     6301 cgcgatgccc gcgccgagcg cggccacgtc ggcggccttg tccacgagcc gcacgggaat
     6361 gcccgtctcg gactggatcc ggtcggcgac gccgcgcagc atgccgccgc cgccggcgag
     6421 cgcgatgccg ccgtcgagca cgtcgtggct gagctcgggc tcggtgcgcg acagcaccga
     6481 gcgcaatcct tcgattaccg gcgtcaccga ctcctcgagc gcggagcgga tctcttcgct
     6541 ggtgacgatc acggtcttgg gcgcgccggt ctcgatctcg cgtccgcgga tctcggcctt
     6601 gagctcctcc tccatcggcg aggccgagcc gacctggatc ttgatgccct cggcggtgcg
     6661 ctcgccgatg accatgccgt actcgcggcg caggaacttt acgatcaggt catcggcgtc
     6721 gaagccgccg acggcgacgt gctgcgagga gaccatgccg cccatcgaga tgaccgcgac
     6781 ctcggtcgcg gcgccgccga tgtcgaccac gcacgagccg cgcggctcgg cgattggaag
     6841 gttcgcgccg agcgcgaccg cgagcggcgc ctcgatcagc cagacctccc gcgcgccgac
     6901 cgacagcgcg gcctcctcca tcgcgcggcg ctcgacctcg gtggcgccgg tcggcacgac
     6961 cagcagcatc cgcggccggg tgatcttggc cgagccggct ttggcgagca cgtgctcgat
     7021 gatgcgctcg gtgatcgcga agtcgctcgc gggtccgcgg cggaacggcc aggtggcggt
     7081 gacggtctcg gggctcgcgg cggcggcggt ccaggactcg cgtccgaccg cgacgcagcg
     7141 cgcgttggtg cgctcgaggg cgaccacggt gggctcgcgc aggacgatcc cgcgctcggg
     7201 aagccacgcc agcgtcgtgg ccgtgcccag atcgagcgcg atgtcgcgga tcacgggcac
     7261 gggatcaccg gggcggggaa ggagtggctg cgcacgagaa ccctttcgga aaaatcggga
     7321 cgccccatga tagccggggg ccccggaccc aaccaccgcc cgaacgggcg ggcctcgccg
     7381 ggaccgacgc tgcgggcgcc gcggggaccg gtggaggggg ccccgctagg ggcgccggcg
     7441 ggaccggcgc agcgcgccca tggcggcgag caatccgacc gtggctgccg cgcctttggc
     7501 caggtccgcc ttgcggacct tgccccgccc gagcgccacc cacggccggt gcgcctcgac
     7561 cgcctcgacc aaggtctcgc ggttcgtgcg cgccggtgtc cagcccgcgg cgcgcatctt
     7621 ggcgttgtcc atcacccacg ggtacatcag gtaggcgatc tcggccggcg gcgcgcccga
     7681 caggttcaag cgccaggccc gttccgcgag cgagtaggcg acggcctcgg gcatctcgac
     7741 cgagcgccgc ccggcgagcg cgagcgcctc gctcagatcc atccagccgt cggcggcgac
     7801 gttgtagatg ccggcgctga gcgcgcggcc gcacgccagg tggagcgcgg cggctgcgtc
     7861 gtcctggtgg accatctgca gcggcggcgc atggccgcgg atggtcagga accgcgggga
     7921 ctggaacatg cgcatcacga agttgtcggt gtcggaaccg aagacgttgg cgagccggag
     7981 cacaacggca tccccggcct cggccgcggc ctcctcggcg tcgagcgcgt tgaccgcgta
     8041 ggcatagtcc gcggagggac gaaccggcga tcgttcggtg agcggaaagt cgttgtcggg
     8101 gttcgcgccg tagacggtcg cggtcgaggc gaacacgagc cgtccgcccg cgacggcgcg
     8161 cgccgcagcg gcgacgttgc gcgtgccgtc cacgttcacg cttcgcatct cgtcgggcct
     8221 tcgcgccgag tccggcgcga aggccaggtg cacgaccgcg tcgcatccgt ccaggaaccg
     8281 cccgatccgc tcgtcgcgga cgtcggattc gaagaactcc atcttcgggc tgccgtcgcg
     8341 tggtgggacc gcgtcgatgc cgacgacgcg ctcgaccgtg gggtcggcat ccaggcgcgc
     8401 cagaaggcat cgcccgagcc ctccacccac gccggtgatc gctatcgtgg tcatcgccgc
     8461 atccacccgc ctgccttgct cgcgaaagcg ccggaacgcg cttcgggcct agaatagagc
     8521 cgtgagcacc gatccttccg gtcttccgcc ggatcttcca cccgacatcc tgggaaggat
     8581 tccgctgttc gccgagctcc agaaggtcct ttcctgggcc ggcgggccgg tgaactggga
     8641 tctggcgcgc caggtcgcgg tcgcggccgc agcggccgcg ggcccgcaga ccgtcgatcc
     8701 ggccgtcgcg gccgagatcg gcgaggacgc gcgcatcgcg cacctgtggc tcgccgagac
     8761 gaccgggatc ggcgaggccg gcgtggttcc gcccgccaag gccgtctcgc ccgccgactg
     8821 ggccgagcac gcgtgcacgg cctaccgcga gatgatcgac ccgctcgcgg cgaaggtctc
     8881 ggccgcgatc ggcgagcagg ccggaagcgc gctcggcgag gacgccgggg cgctgccccc
     8941 ggggctggac cccggcgcga tcggcgccgc gctcagacag atggcgccgc tgttcctcgg
     9001 cgtgcagacc ggcggcgtgc tcggcgcggt cgcccaggac gtgctcggca gccgcgacct
     9061 tccgatcccg ccggccgacg gcgcgccggt gatcgtggtg ccgtcggcga tcgcgcagct
     9121 ggcggacgag tatcagctcg atcgccgcga ggcgctgctg tacgtggtgc tgcacgagtg
     9181 cgcgcaccgc gcgatcgagg aatcgctgtc cacgttccgc tcgagcttct tcgcgctgtt
     9241 ccacgactac gtcgcgtcgc tgtcgatcga cttttcgagc gcgttcgaac ggctgcaaga
     9301 aatagatttc agcaacccgc agggcgtcga ggagcaactc ggcggccaag gcttcttcga
     9361 tctgctcgac tcgccggcgt cgtccgcggc ggccgggcgg ctcgagcatc tgatcgcgct
     9421 ggcggaggcg ttcgccgacc gcatggtcga ggcggccgcc gaaggccggc ttccgtccgc
     9481 ggcgcgtctt gccgaagcgg cggcccggca ccggtcgggc gccgaaggca cggcggcgct
     9541 gcacaagttc atgggcgtgg gcgcctccga cgaggcgcgc cgcgccgcag cgcgcttcat
     9601 ccgagccgtc ctgagcgagg cgggatggcc cgcgctgatc cgaatgtggg aggactccga
     9661 gacgatgccg accaaagcag agatcgacga ccatgcggcc tggctaggga ggatccgatg
     9721 accgcgctgc acgtcggctg cggcggattc cccgtcgaac cggccgagta cgccacacag
     9781 ctgagctacg tcgagatcca ggagaccttt cacgaaggcc ccgagctcga cgcggtcaag
     9841 aaggatctgg agcgggtcgc gggcgacctt cgcatcgggc tggtcgcctc gaaggtgatc
     9901 acccatccgc gcaaggacgg ggtctacaaa aagcccgggc ccgacgtgcc cgaccatgcc
     9961 tcggtcggac acttcacgcg ctcgcgctgg accgacgagg cctgggagcg catggacgtc
    10021 ctggtgcggg cgctcaaggc cgcggtggtc gtgctgcgca cgccgccgtc gttcaagaag
    10081 acgccggcca acgcgctggc gctggagaac ttcatcgcgc acgccgagcg acccggcctg
    10141 gagatcgtct gggagcacgg cggaacctgg cccgagacgg acgcggccga ggtcgcgcac
    10201 cggctcggcc tggtgatcgc ctcggacctg accggcacga tccccgacgg cgaggtctac
    10261 gcccgcttca ccggcggcgc caagggcacc tcggcgctga ccgacgcggc cggggccaag
    10321 gccgcgcaga agctcaccgg ccgcaacggc ttcgtggtct tcgccaacaa gtcgtgctgg
    10381 gaggacgcgc gacgtttttc caagaccgtc taggcgctcg cctgcgtccc tagcggcgag
    10441 tgccttcgac gatcttgagc gcgaacggcc acgacacctc ggtcgtgtcg tagatcgcgg
    10501 tgagggcgag ccggtactgg cccggcggca tcgccggctg ccacgacatc gacgagcccg
    10561 gggtcagcgc cacgatctcg ggcgccttgc cggccgccga tacctccagc cgcgcgcgga
    10621 gcggagcggc cgacagggtg aacaggaccg gcgactcggc cggaccttcc agcgccggcg
    10681 gcgcgggcgg cttgagcagg gcgcagtcct cgccgacgca gtagcgcgcc ggcgtggttc
    10741 gatgagagcc cgagcccagg aacatggcgg cgggcggcag gttcccggga agctcgacct
    10801 cgcccgatcc ctgccgcggc tccgcctccg gggacaccga ggtcttcgcc ggctcgccgg
    10861 cggcgccggt gaagcggggg tccttcgacg gtccgccgct gcacgcgacc agcgcgagca
    10921 gcacgcccaa gctcaacagg gggcgcagcg ggcggcgcag caggcggcgc agcaggcgag
    10981 tatgcatgcg ctcacgatac cggcgcgccg gggccggtcc cggaagccgg gacgctactc
    11041 gtcgagaccc ttggagatca aaaagccgcg tgctcgctcg gcgagcggat accggttcac
    11101 cagccgccag aaggacgggc cgtgatccgg gatccgaaga tgagcaagct cgtgcacgat
    11161 gacgtagtcg cggacccagt cggggcagtc ggagagcttg tggctgagcc ggatcgtgcc
    11221 gtcggccggg gtgcaggagc cgtagcggcc cttcatgttg gcgacgtagc ggatcgagtt
    11281 gagcttggga cggccgtaga agtagcggcg gtgcagctcg gccgcccggc gctgcaggtc
    11341 tcgcttctcg ttgagccgct ggatctgctc ggactcctcg atgcggtcca ccatcgtctt
    11401 gacccagcgt cgcgcctcgg cgcgggtcag ggtggcgggc agcatgaccg tgacccggtc
    11461 gccgtccttg accgcttcca cggtcttgcg gcggcgggga gagcgtacga cttcgacctt
    11521 gaacctcatc gggggagcca ccttctggaa aacggagcgt cgtcggcgtg ggtcggcaca
    11581 tggaatgtcg gcgcggaccg gagtccggtg aagccgtcgt ggaatataac gagcgtcggt
    11641 tcgccacgat tcatatttcc cgtcccgggg gcggccgaac gtccggagcg accggctcgg
    11701 ccggatcctc cacccgcgtg aacgggcggc ctggggtttt cccgctctgg ggcctagagg
    11761 tcctcgatcc cgatgtggcg gtggcccgcc tgggagtggt cggcgagatg gtgccgggcc
    11821 atctcctcgt cgctccaggc ggcctgggcg atctgaatca gggtgccgtt ggcgtgcttg
    11881 gggtggatga aggcctcttt ccactggctg cccgcgatgt tcacgccgaa gggctcgacc
    11941 ccggtggatc gcaggtgctc cagggcgcgc tcgatgtcgg gggtcttgag cgtcacgtga
    12001 tgcacgccct cgccgcgcgc gtccaggaag cgctgcacga agccgtcgcc gagcggcgcg
    12061 acgagctcga tcttgccgcc gaacggaaag cggaactgcg cgaagcgaaa tccttgatcg
    12121 tgctgatccc ccgcgaacag gaacgtcgca ccgagcgcgt cgcaatacag cgacatcgcg
    12181 gcgcgaatat ctcgcacggc gatcgcgaca tggtcgagct gcgcgtcgag cacggcgcga
    12241 actttaccct cacgcgtcgc gttcggcgca agcgcgccgg ggcgggagaa aaagtttgca
    12301 catcgaaaaa tttttgtttc ccggctttcg tgccgcgcgg aatgcacgcg aatggccgtg
    12361 acctgcgcgg ttgcataagc gttggatatc gacctgggtt tttgccggta ttgacgcagc
    12421 ccgtgcggat gagtaacttt ccgccccgca gcgcgctccg ttcggctgag tagagaacgc
    12481 cggtgaaagg ggaaggcacc ggcggcccca aaggtcgatc ggagccgctg ctttttctct
    12541 tccctttacg ttccgtcgaa cgacggcgcg cgcttctcct tcaccgcggc gagaccttcg
    12601 agaagatccg cggtcgccat cgtcaccggc tgcgcgagcg cttcgtactc aagcatcgtg
    12661 tcgagatcgt ttcgctccgc gagctggatg gcgcgcttga cgagccgcgt cgcgaccggc
    12721 gcgttcgcgg cgatctcttc cgcgagcgct ctcgtctcgg cggcgagcga ctcgcgcggc
    12781 accgcgcggt tcaccaatcc catcgactcc gcctcagcgc cgctcaccac ccgtgaggtg
    12841 aacagcagat ccgctgcgcg ggccgagccc accagccgcg tcagcagata cgtgccggcc
    12901 atgcccggat gcaggccgag cttggtgaac gcggccgtga gcttggcgtc ggcggcggcg
    12961 aggcgcaggt cgcacgccag ggcaaggcac aggcccgcgc cgacggccga gccgttgatc
    13021 gcggcgatgg tggggatctc caggtcgcgc acggccagga acttcccgta gaaggcccgc
    13081 atcttggcgc ggatggcggg cacggacgcc cccgggcccg gctggatgaa cgacaagtcg
    13141 ccgcccgcac agaacgcctt gccggcgccg gtgatgatca gaacgcgcgc gtcctggtct
    13201 tcgcgcagat cgtcgatcgc ggccgtgaac gcgtcggtga gatcggcggt catggcgttg
    13261 cgcacgtcgg gcttgttcag cgtgagcgtg acgatcccgc cggatcgctc taccaaaacg
    13321 ggcgcatcgg ccattgcgtt cctccttcga cgtccgggtg taaccgttgc ctctcccccg
    13381 gcgtctgatg ggccagaagg aggtggcgca cagtgtatga attcgcgatc gtagcgctgc
    13441 tgggtctcgc gctgtacaag acggtggact tcctcacggg cctgaccaag atggacgagc
    13501 ccgccatccg taccatggca accctgggac tcggcgtggt cgcaacatgg atcctcgact
    13561 actccatctt cgcggcttgg ggtgtcacca tccgcgaagc ctggatgggc ccggtgttct
    13621 ccggcctgat cgtgggcggc ttcgcctaca tctggcacga cgccctcggc gcgctcgaag
    13681 gctacggccg gcgcgcgcgc gacgaagcgc tcgagatcga gaaccgcatc ccgcgcgcgg
    13741 cctgacgccg cccgcgtcac cgcccacaag gcgaacgaaa ggggccggat tcccccggcc
    13801 cctttcgcat gttcgcgctc agcccaggta cagaaagccg aatccctcgt cgcgaaacgc
    13861 gatgccggcc ttctcgagcg cctcgctgta gtcccccgtg cattccgggt cggactcctc
    13921 catcccgtga tcggccagca cgacgtaggc cgtgtcgtcg tcaccgatcg cgtccatgat
    13981 ctcaccgagc cgcgcgtcgg tgtcccgcag cgccgcgtgc ccgatgtcgc tgtaggggcc
    14041 gccggcgtgg ttgccggcgt cgttgaggat caggttgacc cacatgaacc gcggacgcgg
    14101 gttgccggcg tcgccgcgcc acaacgctcc cgcctgctgc accgccatgt agtcggccgc
    14161 ggttgaccat gcgtactcgg gcttggtccg gacgaacgtc tgggtcgcgc cggggatcgc
    14221 ggtcgggtcc ggcagcgcgg tctgcagacg ctggacgacc tctcccccgt cgcggaccgc
    14281 gtcgaaggtc gcgtaggacg cgccgcggtc gcagggctcg ttgatcgagg cggtgaagcc
    14341 cgcgccgctg cgcgcgagcg cctcgtagac ggtctcgacg tcggaggcga tctcgttgcg
    14401 ggccagatgc caggtcgcgg gcgaattggt gttgacggcc cggccggtgg cgcgctcgta
    14461 gtaggcgttg tgcagcaccc cgtgcacgcc cgggtgcacg cccgtgagcg cggtggtgtg
    14521 gttggcgagc gtgaccgtcg ggaacgaggc gatcagtccg tgctcgtaga cggtgccggc
    14581 atccatcagc ttcgcggtgt tgggaagctc gccggcgcgc gccatcgcgt agagcacgtt
    14641 cgcgttggtg ccgtcgcaca ggaatgccac cacccgcttc ggcgggtcgt ccagatcgag
    14701 gatctcctcc agcacgcggc cgtgctgccg cgcgatcagc ggcccgtccg gccggggccg
    14761 gccgccggcc agcagcgtca gcgtcggcgc gatgtccacc atgcgcgcgg cgcgcgggat
    14821 cgagccgagg cggcggatgc cgcggccggc cgcgatcagc ggcgcacgcg actggatcag
    14881 gttgggtgag ccgtgctcgc cgcggtggcc gccccgctcc tcccagttgt gggcgggcgt
    14941 gtgcaccacc gcgatgtcgg gcgcgtgcgg gtggtcgaac aggtgcgcga tgttgtcgta
    15001 ggcgaacggg tagtggttgg cgcggttgga cggccgcacg ttcgcgcgct cggcctccag
    15061 cgggctcatc gcgccgggat cgccgtgctc gagcgggttg cggccgttca tgtgcagcgg
    15121 cccctgcgcg gtgaaagcga ccatgccgtc ccgggcgtgg gcctcgacga tcccgtttcg
    15181 catgcacagc acgagatcca cgatcccgtc gcactcgtcg ccggtcagga tcgcgatcgc
    15241 atcgtcgagc cggtcgctca tcggtgcgac gactatactc gtcggacatg cctgcgcgga
    15301 cgatcttgca cgtgctgccc catacgcatt gggaccgcga atggtacgag ccgttcgaga
    15361 gctaccggtt ccgcttggtc cgcaccgtcg acgcgctgct ggacctgctc gacgtgcggc
    15421 ccgggtttca cttccatttc gacggacaga ccgccgcgat cgaggactac ctcgccgtgc
    15481 gtcccgaggc tgcgcctcgc gttgccgcgc acgtcgcggc gggacgcttg ctggtcgggc
    15541 cgttccgcat cctgatggac gagttcctgt gctcgcccga gacgatcgtg cgcaacctcc
    15601 ggcaagggct ggcgaccgcg acccggctcg gggtgcggcc gcgcatcggc tacatccccg
    15661 actcgttcgg ccacgtcgcg cagatgccgc agatccttcg gctcgccggc atgcaggatg
    15721 cgctcgtgtg gcgcggcgtg cccgccgcga tcgaccgtcc cgccttcacc tgggtcgcgc
    15781 cggacggcac cgagatccgc acgctctaca tggccacgtc gtactcgaac gcggcgaccc
    15841 tgcccgagac cttcgaggac ctgatggtgc gggccaagcg catcgtggcc gacatggagc
    15901 cgttccggcc cgagggcgtc gtgctcgcga tgaacggcag cgaccatctc gcgccgtcgg
    15961 cgacgctgcc ggcggcgctc gatcaggcca acgaacgcca ggacgagatc gagtttcgca
    16021 tcgcgccgct cgccacctac gcggcggacg cgccgcgcga gaacctgccg cgctggcacg
    16081 gggagatgcg ctccagcgcg cgcgccaacg tgctgatggg cgtgctgtcg gcgcgcatgc
    16141 cgctcaagca ggccgagctg gccgcgtccc gcatgctgga gcgctacgcc gagccgctcg
    16201 ccgcgatcgc cggctttgac ccgggcggca tgctcgacga ggcgtggctc ggcatggtgg
    16261 agaacagcgc gcacgactcg gtctgcggat gcgggatcga cgcggtcgcc ttcgccgtcg
    16321 cctcgcgcta cgagcgggcc gagcgcatcg gcgctggcgt gcgcgacacg gcgctgcaga
    16381 tgctcgccga gcgcgcggcc gttgccgcgc ccgacggacg gcccgacacc gaaggcgtgc
    16441 tgctgtggaa cccgtcgccg ttttcgcggg ccggcgtgtg cgaagcggtg atggcgctgc
    16501 cgggccacgg cggcgacgcc gagctcgccg ggccggatgg ctcgcgccac ccggtgcagc
    16561 gcctggccga gaccgagcag gtcgtggtgg acatggtgct gcgcggcgcg gagctcgcgc
    16621 gcatcgtgcc gacgattcac tcgcgcatga tgggcacgct ctccgtgaac gcgatgacgc
    16681 tcaccgacgg ccgcgtgcgc acggtgcgtc tcgagctcgg cccgatcccc gtcgggcact
    16741 tcgacgtcga ggccgccaaa cgcgaggtcg aggaggccgc gaagcgctca ccgcgcgcga
    16801 agttccacgt gatcggaaac ggaccgccgc tcgcgcggct gctggtggac gcgcccgaga
    16861 ccggcgggct cggctggacg acgctgtttc ccgtcgcggc cgaagccccg gcgccgacgc
    16921 gcgtgtccgc cgaaagctcg acgatctcga acgagcacgt gcgcgtcacg ctcgacgagc
    16981 agggccgtgc gaccgtcgag gctgcgggca tgcgtctgga cggcatcggg ctcgtggacg
    17041 gaggcgaggc gggtgacgag tacacctact cgccgccggc gcgtgacacg gtcgtgagcg
    17101 agccgcgatc gtgcaccaac gagatcgtgt ccaccggccc gctcgaggcg cgcatccgct
    17161 cgtggcagac ctggcgcgtg cccgccgcgc tcgatccgtc ccgcacgcgc cgcgcgcggc
    17221 gcaccgtcga gatgcccgtc gtcgtcgagt gggcggtgcg cgccggcgag ccgttcgcgc
    17281 gcgtggacgt gcgtgtggac aacaccgccg ccgatcatcg ccttcgcctg cacgtgccgc
    17341 tgcccggccg cgtcgagggc tcgcacgccg acaccgcctt ccacgtcacc gcccgcgggc
    17401 tcgacggcga aagcggctac gagcacgcca cgccgacgtt cccgtcgcgg cgctgggtgg
    17461 atacgagcga cggagcccgc ggcgtcgcgg tgctccacac cggaacgccc gagtacgaga
    17521 tcaccggcgg aacgcagatc gcggtgacgc tgctccgctg cgtcggctgg ctgtcccgtc
    17581 aggacctgac gacgcggtcg ggtccggccg ggccgatgct ggccgcgccc ggcgcgcagc
    17641 tgataggccc gcacacgttc tcgctcgccg tgtatcccca cgagggcgac tggcatgccg
    17701 gcaacgtgcc ccgcgtcgcc gagacgttcg gccatccgtt cgccgcgctc gcgctccgcc
    17761 cgcacgacgg cgaccttccg ccatccgggt cggcattgcg cgtcgagccc gcctcggtgc
    17821 agctttccgc gctggagcgg cgtggcggcg cctgggaggc gcggatctac aacgcctcgg
    17881 acgcggcctg cgaggcgcgg ctgtcggcgg gcgcgccgct cgcggcgcac gaggcctgga
    17941 cggtggacct gacgggcgag cgcgcgacgc cgctgccgtg cgacgcgggc acgatctcgc
    18001 tcgtgctcag gccctggcaa atcgaaaccg tccgatttgt gccgcgcgaa gcgacctgaa
    18061 gctttggcac aatcgtctcg gtgacccttc cccgcgtgct tgtccttgcg gtcgcgctga
    18121 gcgcggccgc ttcgtcgtgc agcgccggtc cgccgccccc cgagcgcacc cgcaccgtca
    18181 tcgtgcacgc ggccaaggcg cttccgtcct cgttgccgtc cacgctcgag gagatgcagg
    18241 gcgtcaaatg gattcgcgtc gcgaccaccg cgatggtcaa cctcgtcaag gtcgagggcg
    18301 gcaagctgct ccagccccgg gccccatcga gcgtgctgcc gttctcgatc ggtgcggccg
    18361 agccgctccc cgccgacggc gatgcggtct cggcgtggct ggcgcagggc acggcggtcc
    18421 tatccacggc gtcggcgcgg cttcgcggcc tgcggcccgg cggcgcgatc acggtcgggc
    18481 tcggcgacaa gcgcgagcgc atcgtcatcg gcggcgtgcg ggacgacgcg cgcaccagcg
    18541 gcgtcgaggc gctcgttccg ctcgcagtgg cgagccgcct cggcgtgacc gaggtgcggc
    18601 aggtagtcgt cgaggtgacc gagtcgggct ccgcgccgct ccaggacacc gcccgcatgc
    18661 ttgccgcgcc ggtgccggtc cgtgtgggat cgcgtgagga cgtcgcgacg cccgatgccg
    18721 gaaagctgct ctcgctcgcc gagatcaagg aacgcttcgg cgagttctcc ttcgtggacg
    18781 taccgggccg ctggctcgag ccggacccgg catgggagga ggccaacatc gcgacgcagc
    18841 acgtcccgat cctgggacgg atgcggtgcc acaagaaggt catggggccg ctgacggccg
    18901 cgatgctcga ggtgcaagct gctggcctgg agcatctggt cgtcgagccg tcgttctgtt
    18961 acgcgccgcg tgtccagttc ggcgacgccc ggtcgatctc gcgtcacact tggggcatcg
    19021 ccgtggacat caacccgacc ggcaacgcat tcggcgacga gccgacgcag gatccgcggc
    19081 tggtggcgat catggcgaag cacggattct cgtggggcgg cgtgtggatg acgcccgacg
    19141 gcatgcactt cgaatacacg ggcctgtaag gaaacccacc gatccttgca acacgacgcg
    19201 gctctggttc gtcgttcata gtgatggcaa tggtcggcgt ttcgcgcgaa ggcctctccg
    19261 agccggtctc cggacggctt gcgggcctgt acgccctcta cgggcacgac ctcaagcggc
    19321 tcgcctttct gatgaccggc gatgcaagcc tcgccgagga cttgatgcac gacgcgttcc
    19381 tgcgcatggc cggccgcctc ggccatcttc gccaggaaga ccgcgcgctc gcgtacctgc
    19441 gcaagaccgt gatcaacctg gttcgcgatc gcggccgtcg ccgtgaccag acgccgctgc
    19501 ccgacccgcc gcacgaacga gacctcgatg ccgagcagga cctgcgcgac ctgctgatga
    19561 cgatccccgc gcggcagcga gccgcactgg tcttgcgcta ctacctcgac ctgcccgacg
    19621 acgaggccgc cgacgtgctg cggtgccgcc ccgcaacggt tcgctcgctc gtggcgcgcg
    19681 gactgtcggc ccttcgaatc aacagcgagg acgaccatgg atgagcgact gtttcgcgag
    19741 cggctggcac gcgccgccgg gcgcgttccg cctccggcaa ccgaccttca ggatctgcgt
    19801 cggcgcggcc atcgccgcgc ggcccgcacg atcgtcgcgg ccctcgcggc cgtcgcgctg
    19861 gtgaccgccg gcgtctcgac cgtcgcgctc acgcgcacgg cccgccgagc cgaaccggtc
    19921 accccaccgg ccgcgaccgg tccctgtga
//
LOCUS       DATLFO010000143         6465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_132269, whole genome shotgun sequence.
ACCESSION   DATLFO010000143 DATLFO010000000
VERSION     DATLFO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132269"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VM841_05500"
     CDS             <1..405
                     /locus_tag="VM841_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVL89674.1"
                     /translation="FGNAVDFARATRLAAVSSRRGEDAIDDLKLARERAVAGGSQLEA
                     SRIEAELRARGVRPRAGRPKKSGSAGRGGLSVREEEVATLVAAGASNADIAARLFLSE
                     RTVQDHITHALRKLALGGRAGLAAWAAKNGLV"
     gene            791..2200
                     /gene="cysS"
                     /locus_tag="VM841_05505"
     CDS             791..2200
                     /gene="cysS"
                     /locus_tag="VM841_05505"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVL89675.1"
                     /translation="MGLLVYNTLTRRKEPFPKDPGEQVGMYVCGPTVQDQPHLGHVRA
                     AVSFDVIRRTMLALGYRVLFVMNVTDVDDKIIARGVEEGRSSWEVAEQYSRVFDSTMR
                     ALGVLPPDIAPRATGHIAEMVALIIDLVDRGMAYPVEGGDVYFAVERFDGYGKLSHRS
                     LDDMRAGERVEPDPRKRHPMDFALWKAAKPGEPAWPSPWGDGRPGWHIECSAMAMKYL
                     GRTFDVHGGGMDLIFPHHENEIAQAEAATGEPFVRVWLHNGFVTLDKEKMSKSLKNYV
                     SAADVLRDFPAPVVRYFVVAGHYRSQIDFSVDSLEDARGAWARFTGFARNARSVLAGA
                     PLPEPDRSSPWREKFLEAMQDDFGTPEALAVLFDFIGEGNQIVASAERSGSHDDLARA
                     LATFSELSGILGLDPVAQWPQTQQANLGPLVEHLLADREAARAGKDYARADAIRDVLK
                     QAGIAVEDRPGGARWYPLS"
     gene            2205..2990
                     /gene="rlmB"
                     /locus_tag="VM841_05510"
     CDS             2205..2990
                     /gene="rlmB"
                     /locus_tag="VM841_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HVL89676.1"
                     /translation="MRIEGRNPVREALRANVHVKRLLVASGVEERGAIEEILALAQQR
                     HIEVRRVPRAELDESAETGANQGVVAEASDYVYRPWQDAVAVAEERGEAPLLLALDGI
                     TDPQNTGSLIRSAHMLGAHGVLLPSRRAATVNATVAKASAGAVFYTGVDLVTNLERAL
                     AECKKAGLWIVGLDGSGDTEVSNCPLLSEPMVLVVGAEGAGLSRLLRERADVLIRIPQ
                     RGSIDSFGASVAGAIALYAAAGARSLSAGGTQLSASHPDAKPT"
     gene            3139..3780
                     /gene="sigH"
                     /locus_tag="VM841_05515"
     CDS             3139..3780
                     /gene="sigH"
                     /locus_tag="VM841_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HVL89677.1"
                     /translation="MHDEILDDVADEDLVIRARSGDEAALEHLLIRYKNIARVKARSY
                     FLVGADREDIVQEGMIGLYKAIRDFDSEKQTSFRAFAQLCITRQIITAIKTATREKHI
                     PLNSYVSLNRPMSQDDGGEPDRVLVDVLSTHASSDPVEMVISSEEVRTMQTSFSEILS
                     DLEAHVLNLYLEGKSYQEIAASLRRHVKSIDNALQRIKRKVEAHMERRRLEAV"
     gene            complement(3927..4367)
                     /locus_tag="VM841_05520"
     CDS             complement(3927..4367)
                     /locus_tag="VM841_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89678.1"
                     /translation="MRRFIAACATAAVCALPASVLAADPSASCNSGIIPPSSSPAGAG
                     IRATTEPMAATVTACSTQAPLPGHARVRLDVSDPSILIVADGDPAANAGGCTDGYTAA
                     RVDGTGPSLYHQPDGGYTSRARAKSPAEIADSAVKHCTLAPASA"
     gene            4576..5742
                     /locus_tag="VM841_05525"
     CDS             4576..5742
                     /locus_tag="VM841_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89679.1"
                     /translation="MRRTAFVLALLLLVPLEGGATPRELPRPLPMPAMSYDGAGFTVP
                     VNGQIVTIARQGSRHADDPTGRIAAYTFIDGEMDWPPHTIIDTELDDRNPAGGLTRSG
                     ALIVFYARAVNKHEWVDMRAARIVGDEVVETIIPTEPDSGFSPYGPLVVLPSGKLMQT
                     VYGRGVENGRTYYRIRAFFSTDDGRTWPEERVVWKTYTEMPNETAAIWVDGATDSASR
                     LMAFASSARRLNDEWVHYVMQIVSTDGGKTWRRLGWVSGISDRGMVPWAANLGGGRLA
                     LVTVDRREYTINVAMAWSRTVFGQPSWWPRGRAVYQSTFMSTERVNVEGIGQTKNWSD
                     FGYPSIVSLGPKEYHKFIVFYDSRTGSSGMSPADVPADTDLILTRLFARPLNER"
     gene            complement(5756..5828)
                     /locus_tag="VM841_05530"
     tRNA            complement(5756..5828)
                     /locus_tag="VM841_05530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5793..5795),aa:Thr,seq:tgt)
     gene            complement(5869..>6465)
                     /locus_tag="VM841_05535"
     CDS             complement(5869..>6465)
                     /locus_tag="VM841_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89680.1"
                     /translation="GSMIPRLDRLLSDLLDLDRLSRGIIEPKRRPVDLGGLVRGVVRD
                     FRTSFGRQVVSDIAPVVADVDRAQIERIVENLLTNAARHTPEDTPIWVTVEESPEGPA
                     IVVEDAGPGIPENLRRDIFEPFRQGPSIRAHSPGVGIGLALVRRFVDLHGGTVEVSER
                     RGGGSSFRIVLPRAQTGRAPRPTLTVETPAASPRSHAG"
BASE COUNT         1034 a         2088 c         2277 g         1066 t
ORIGIN      
        1 ttcggaaacg cggtggactt cgcccgggcc acccgtctgg cggccgtctc ctcacgccga
       61 ggcgaggacg cgatcgacga tctgaagctg gcgcgcgagc gagccgtcgc gggaggttcc
      121 cagctcgagg catcccgaat cgaggccgag cttcgcgcgc gcggcgtgcg gccccgcgcg
      181 ggccgtccga agaagtcggg gagcgccggc cggggcggac tgtccgtccg cgaggaggag
      241 gtggccacgc tcgtggccgc cggcgcgtcc aacgccgaca tcgcggcgcg gctgttcctg
      301 tcggagcgca ccgtgcagga ccacatcacg cacgccctgc gaaagctcgc tctgggcgga
      361 cgggcgggcc ttgccgcctg ggccgcgaag aacggcttgg tgtagtccgt cgcgtccgtg
      421 cgccgggacg gtttcgggcg cattgggcgg gatcgcaggg atgtaccgtc cgattcgccc
      481 ctatccggcc ctccccaagg atggggtgga aaccatcccg attgactatg cgagaggtcg
      541 tgcgcgtccc cgatatcaca agcgtccggc ttggtgagaa ggggaaacga catgaaggca
      601 acgcggaagt tcgtcgtggc gatcgcggcg gcgatgtgca ctttggtcct gacgggtggc
      661 gcggccaacg cgctgccgtt cgtcaaccag tggccgtaaa gtgccgctcg ggtcgcgccg
      721 gccgctttgc ggccggcgcc tttcgttgcc ctcgtccgcc tcggggcgac gctgatagag
      781 tccccgcgcg atgggcctgc tcgtctacaa caccctgact cgccggaaag agcctttccc
      841 gaaggacccc ggcgagcagg tcggcatgta cgtctgcggg ccgaccgttc aggatcagcc
      901 gcacctcggg cacgtgcgcg ccgccgtctc cttcgacgtg atccggcgca ccatgctcgc
      961 gctcggctac cgggtgctgt tcgtcatgaa cgtcaccgac gtcgacgaca agatcatcgc
     1021 ccgcggtgtc gaagagggcc ggtcctcctg ggaggtcgcc gagcagtatt cgcgggtgtt
     1081 cgactcgacc atgcgcgcac tcggcgtgct gccgcccgac atcgcgccgc gcgccaccgg
     1141 tcatatcgct gagatggtcg cgctcatcat cgatctggtg gataggggca tggcatatcc
     1201 ggtcgaaggc ggcgacgtgt acttcgcggt cgagcgcttc gacggctacg gaaagctctc
     1261 tcaccggtcg ctcgacgaca tgcgcgcggg cgagcgggtc gagcccgacc cgcgcaagcg
     1321 tcatccgatg gacttcgcgt tgtggaaggc cgccaagcct ggagagccgg catggccgtc
     1381 gccgtgggga gacgggcggc cgggctggca catcgaatgc tcggcgatgg cgatgaagta
     1441 cctcggacga accttcgacg tgcacggggg cgggatggat ctgattttcc cgcaccacga
     1501 gaacgagatc gcacaggccg aggcggccac gggcgagccg ttcgtgcgcg tctggcttca
     1561 caacggcttc gtgacccttg acaaggagaa gatgtcgaag tcgctgaaga actacgtgtc
     1621 ggccgcggac gtgttgcggg acttcccggc gccggtcgtg cgctacttcg tcgtggcggg
     1681 ccactatcgc agccagatcg acttctccgt cgactcgctc gaggatgcgc gcggcgcgtg
     1741 ggcgcgtttc accgggttcg cccgcaacgc gcggtcggtg ctcgcgggcg cgccgctgcc
     1801 cgagccggat cgttcctcgc cctggcgcga gaagttcctg gaggcgatgc aagacgactt
     1861 cggcacgccc gaagctctcg ccgtgctgtt cgacttcatc ggcgagggca atcagatcgt
     1921 cgcctccgcc gagcgcagcg gcagccacga cgacctcgcc cgtgcgctgg cgacgttctc
     1981 cgagctttcc gggattctcg gactggatcc ggtcgcgcag tggccgcaga cgcagcaggc
     2041 gaacctcggg ccgctggtgg agcatctgct ggccgaccgt gaagccgcgc gcgcggggaa
     2101 agactatgcg cgagcggacg ccatccgcga tgtgctcaag caggccggca tcgcggtcga
     2161 ggaccgcccc ggcggcgccc gctggtatcc gctgtcctag cgtgatgcgc atcgaagggc
     2221 gcaacccggt tcgcgaggcg cttcgcgcca acgtgcacgt aaagcggctg ctggtcgcat
     2281 ccggggtgga ggagcgagga gcgatcgaag agatcctcgc cttggcgcag cagcgccaca
     2341 tcgaggttcg gcgcgtgccg cgtgccgagc tcgacgagtc cgccgagacc ggcgcgaatc
     2401 aaggcgtggt cgccgaggcc tccgactacg tgtaccggcc gtggcaggac gccgtcgcgg
     2461 tcgcggagga gcgcggcgag gccccgctgc tgctcgcgct ggacgggatc accgatccgc
     2521 agaacacggg atcgttgatc cggagcgccc acatgttggg tgcccacggc gtgctgctgc
     2581 cctcgcggcg ggcggcgacg gtcaacgcga ccgttgcgaa agcctcggcg ggcgcggtct
     2641 tctacacggg ggtagatctg gtcaccaacc tggagcgggc cctggccgag tgcaagaagg
     2701 ccggcctgtg gatcgtcggg ctggacggaa gcggggacac cgaggtatcg aattgccccc
     2761 ttctgtccga gccgatggtt ttggtggtcg gcgcggaagg cgcaggtttg tcacgactgt
     2821 tacgtgagcg cgcagatgtc ctaattcgga ttcctcaacg cggctcgatc gactccttcg
     2881 gcgcgtcggt ggcgggggct attgctctgt atgccgctgc gggagcaagg tcgctttcgg
     2941 cgggtggtac tcaattgtcc gcttcgcacc cggacgcaaa accaacctag ttccaaatga
     3001 ctagttcgtt cccttgaccg tcgaaaaggg tcgcgtctac cctgtgcatc gggcgctcca
     3061 caggggctcg gttgggtaag ggcaactctc ctagcggcgg gggagcatat agtgcggcca
     3121 gatcttgatg taactcgttt gcacgacgaa attctcgatg acgttgccga cgaggacttg
     3181 gttatccggg ctcgttccgg cgacgaagca gcactcgaac accttctgat ccgatacaag
     3241 aacatcgcac gcgtgaaggc gcgttcgtac ttcttggtag gggcggatcg tgaggacatc
     3301 gtccaggaag gcatgatcgg cctgtacaag gccatccgcg acttcgactc ggagaagcag
     3361 acttccttcc gtgcgttcgc gcaactgtgt atcacgcggc agatcattac ggccatcaag
     3421 acggccacgc gcgagaagca cattcctctg aactcgtacg tgtcgctcaa ccgtccgatg
     3481 tcccaggacg acggcggcga gccggaccgc gttctggtgg acgtgctgtc cacgcacgcg
     3541 agctcggatc cggtcgagat ggtcatctcg agcgaagagg tccgaaccat gcagacctcc
     3601 ttctccgaga tcctttccga cctcgaagcg cacgtgctga acctgtacct ggaagggaag
     3661 tcctaccagg agatcgcggc ctctcttcgc cgccacgtga agtcgatcga caacgcgctg
     3721 cagcgcatca agcgcaaggt cgaggcccac atggagcggc gtcgcctgga agccgtctga
     3781 tcggcgcatc gatttcgtag agccggccgt cacggccggc tctaccattt ccaggcgttc
     3841 gtgacgatgc gacgtgagtc gtcgtccctg tccgtagggg tgccgggacc ggacgcgcgt
     3901 ccggtcccgg ctgcttcggc ggcgggtcag gccgaagcgg gggcgagcgt gcagtgcttg
     3961 accgccgagt ccgcgatctc ggccggcgac ttcgcgcggg cgcgcgaggt gtagccgccg
     4021 tcgggctggt ggtagagcga cgggccggtg ccgtccaccc gtgcggcggt gtagccgtcg
     4081 gtgcatccgc cggcgttcgc ggcgggatct ccgtccgcca cgatcaggat cgacggatcg
     4141 ctgacatcga gccgcacccg tgcgtgcccc ggcagaggcg cctgcgtcga gcaggccgtg
     4201 acggtggcgg ccatgggctc cgtggtggcg cggatcccgg cgccggccgg agacgacgac
     4261 ggagggatga tgccgctgtt gcaggaagcc gagggatcgg cggccagaac gctcgccggg
     4321 agcgcgcaga cggcggcggt ggcgcacgca gcaatgaagc gtcgcatgat ggatgtgtcc
     4381 tttcggatcg tcggtcgtcc cgtagggacg gcgttcgcgg cgcgaatgcc ggcgcagtgt
     4441 ctcgcgcctc gcgcacgcgg tcccgtacgg gcagatcggg agactcggaa cgaacggcgg
     4501 ccggatcgcg agcctgctct gctctggtcc cggtgacgtg aaggatcggc ccgttcggtg
     4561 ttgaatgtct cggacatgcg ccgaactgcg ttcgtccttg ccctgctctt gctcgtcccc
     4621 ctcgaaggcg gggcgacgcc gcgggagttg ccgcgtccgt tgccgatgcc ggcgatgtcc
     4681 tatgacgggg ccggctttac cgtgcccgtc aacggacaga tcgtgacgat cgcacgtcag
     4741 ggctcgcggc acgccgacga tccgaccggg cggatcgccg cgtatacgtt catcgacggc
     4801 gagatggact ggccgccgca taccatcatc gacaccgagc tcgacgaccg aaatccggcg
     4861 ggcgggttga cgcgtagcgg tgcgctgatc gtcttctacg cgcgcgccgt caacaagcac
     4921 gagtgggtcg atatgcgcgc cgcgcgcatc gtcggggacg aagtcgtcga gacgatcatc
     4981 ccgacggagc cggacagcgg gttctcgccg tacggtccgc tcgtcgtgct tccgagcggc
     5041 aagctgatgc agacggtgta cggccgcggt gtcgagaacg gcaggaccta ctaccgcatc
     5101 cgcgcgttct tctcgaccga cgacggacga acgtggcccg aagagcgcgt ggtgtggaag
     5161 acctacaccg agatgccgaa cgaaaccgct gcgatctggg tggacggcgc caccgactcg
     5221 gcctcgcgtc tgatggcgtt cgcgagcagc gcgcgccgtc tcaacgacga gtgggttcat
     5281 tacgtgatgc agatcgtctc caccgacggg ggcaagacct ggcggcggct cggatgggtc
     5341 agcggcattt cggaccgagg catggttccg tgggccgcga acttgggggg cgggcgtctc
     5401 gcgctggtga cggtggaccg gcgcgaatac accatcaacg tcgcgatggc gtggtccagg
     5461 accgtgttcg gccagccgag ctggtggccg cgcggccgcg ccgtctacca gagcaccttc
     5521 atgtcgaccg agcgcgtcaa cgtcgagggg atcggccaga ccaaaaactg gagcgacttc
     5581 ggctatccgt cgatcgtgtc gcttggcccg aaggagtacc acaagttcat cgtgttctac
     5641 gactcgcgca ccggaagctc gggcatgagc ccggccgacg tgcccgccga cacggacttg
     5701 atcctgacgc ggctgttcgc gcgtcctctc aacgagcggt gatgcgaggc ctcagagctg
     5761 gggatgggaa tcgaacccac agcctgctga ttacaaatca gctgctctgc cgattgagct
     5821 accccagcgc accgacatca tgccacgcgg agcgtggccg gtcgccggtc agcccgcgtg
     5881 ggaccgcggc gaggcggccg gtgtctcgac ggtgagcgtc ggccgtggcg cgcggcctgt
     5941 ctgcgcccgg ggcagcacga tccggaacga cgagccgccg cctcttcgtt ccgagacctc
     6001 gacggttccg ccgtgcagat ccacgaaccg gcgcacaagc gccagaccga tcccgacgcc
     6061 gggcgaatgg gcgcggatgg agggaccctg gcggaacggc tcgaagatgt cgcgccgaag
     6121 gttctccggg atgcccggac cggcgtcctc gacgacgatc gcgggtccct ccggtgactc
     6181 ctcgacggtc acccagatcg gcgtgtcctc gggggtgtgg cgagccgcat tggtcagcag
     6241 attctcgacg atgcgctcga tctgcgctcg atccacgtcg gcgacgacgg gcgcgatgtc
     6301 ggacacgacc tgacggccga acgacgttcg gaagtcgcgc acgacgccgc gcaccagtcc
     6361 tccgagatcg accggccgtc gtttcggctc gatgatcccg cggctgagcc ggtccaggtc
     6421 gagcaggtcc gacaaaagcc ggtccagccg cgggatcatc gagcc
//
LOCUS       DATLFO010000144         7632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_55034, whole genome shotgun sequence.
ACCESSION   DATLFO010000144 DATLFO010000000
VERSION     DATLFO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55034"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VM841_05540"
     CDS             complement(<1..436)
                     /locus_tag="VM841_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89681.1"
                     /translation="MRGNKTRRMGLILAAVPFVVALGFASSAAAPELPVDRPAVPTPA
                     ERQTPAGGNDLVPTTPPTVQPPGVVPSPLAYRIHSKYRAPSGDIVEKFTPAVLLVPTP
                     VDVDGTLEGNQVPDVLAQLVIDAGRATFRVTAFPGGTTQLPLL"
     gene            complement(465..2522)
                     /locus_tag="VM841_05545"
     CDS             complement(465..2522)
                     /locus_tag="VM841_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HVL89682.1"
                     /translation="MRRWASAAAWAAALSVAGLPAVQAQTPAYDWTPVAIQQPPATMH
                     PGMTVALTVALRNEGTQTWFADGAGPAPVRLGTSSPRDRSSAFAGQDPSWLWSSGKRI
                     RMTTPVVPPGGVGTFTFTFRAPEQPAYPVTLAERFLPLVEPGGWMEGRPEIVLTTIVR
                     PASGAAPAIPPGPGVPQPPALPTPPAGYSWEPVLVPPAPATMHPGAKTVLSVWLRNTG
                     SVAWDADNFTATPVRLAAVNDVGVQPRERASAFGREDPTWLWSGGRRIRMITPRVEPG
                     AIGAFEFTFSAPAVPPYPRNYIERFAPIVETVGLLDDKLITFATQVRPAAGPVPAVPE
                     VPGTEPLPIPTLTPPPVPTDPPLPTPVPTPAPTLPSTPGTECATTMAPRIQHLWPPAA
                     ALSYSPSGTLLTGTIGASQAVTYGLGKLFVADTANDRIVRTDLAGFSADVFGSAGSGE
                     GQMRWPGGIAISDSGIVFVADTGNHRIQQWGFSGLYLGTFGFQGAAPGQFSSPRGIAF
                     IANGPLQGLLAIADTGNDRVQIISRTGVPVAVIGGPGAEPGKLSSPTGVSVWGDEIFV
                     ADTGNNRIQKFAVDGRFLGSFGGEGEGDGCTMLPTGILASQYGVFVTESGSGRVDQFT
                     RWGLHLRTVGGLATPSAVAMDGSAKLYVTRTGSAGLVRLNPLASAQPPSVCQETDPIE
                     CVT"
     gene            complement(2548..5382)
                     /locus_tag="VM841_05550"
     CDS             complement(2548..5382)
                     /locus_tag="VM841_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL89683.1"
                     /translation="MSNTLGGRRLIAFATLGLVLTAVLAVAPAPALGNGVLSGPAYPP
                     PGGATVTSNSCTNAFGFSTPCEGQTPGRNLSYTVTNPGLFTHTYWGPSQLPQLSLDSA
                     GAPGCTPGPTCSNSPVTIPFAAISGTTARWECANNCVGIQIFSGGGSSIQMVRARVLA
                     TVTSGPAWVLASSVSGLPNPANASAVLPVTGVGSFSINYKFEISTNSGSTWTPAIAYY
                     DQSATNPAKHTYIAWGFGFYYNRPPVAANQSFTTNEDTAVGITLNASDIDGNDLHYSI
                     GSPSNGTLSGTAPNLIYTPSLDYVGPDSFTYTATDGFATTGGTVSIEVAPVNDAPVAI
                     NQAITTDEDTPVTFTVSGTDTDTPPENLVYEVVGLPSNGTLDLTDMPTITYTPAQDFN
                     GADSVTFDVFDGALHSSPLGLISITVNPVNDAPVATDSAVTTDEDTPLEITMQATDVD
                     LDTLTYTVLSGPENGSFSPDPDGTSSITYVPNENFFGSDSFTFQVTDGLLDSQIATVS
                     ITVNAVNDAPVADAQELTTDEDTPLAITLTASDVDNELEDLSYSVVNGPTYGSLSGEG
                     AELLYTPNADWNGTDSFTFVANDGELNSVEATVTIVVSPVNDAPVADDNISVETAEDN
                     SVEIVLTGFDIDTDVENLAYSIVEGPAHGTIECSGTNCTYTPDADYNGPDSFTFKIND
                     GELDSNVATVSINVTPVNDAPIAFDQTVLTNEDTATPIVLGGSDVDGDEIVFAIVDGP
                     THGTLDVLDGNTIVYTPFENYYGPDAFTFTVSDAEYTSNIATVSIDVAEVNDNPVAVG
                     DGVSTEQYTPVTFDVLGNDTDVDGDVLTVVSASPGAHGTTTVNPDGTLTYSPSPTFKG
                     EDTLTYSISDGRGGTAIGSVIITEIGCGEDGINTLDGTPAEGAASETIDRQVEPVIGM
                     VDPDTAATVHAHNCTTVVSAENAIDELAGG"
     gene            5639..7456
                     /locus_tag="VM841_05555"
     CDS             5639..7456
                     /locus_tag="VM841_05555"
                     /inference="COORDINATES: protein motif:HMM:NF023252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HVL89684.1"
                     /translation="MDSLNESFRTTLVGGGLVSEGVGLARRNGGPASGSITISGIPEG
                     SSVAKALLYWSVIAGDDPVVTLNGSGVLGGQIGRSPETCYFNSRDSSHDNRVYRADVT
                     SIVTGNGTFTVAGVGDVLSPADGQGASLLVLYKNESADFQRTIIINDGAATGGNPGQH
                     YGSIAHTFGSLPTARPVLGAKLHFGFGDGQPEFPEGAMTVGGQQVLGPNSLSGADGPS
                     WDDAAVPVPASLFPVGTSSVLNRLQVADTSGGDCVLWTYAALVLEQQKSLVPAPTIVI
                     TSPEPGTQQTSPVTIEGTSTNASGIVLTEHGQTLATTTPSGSGAWSVSVALAGGHHDV
                     TATALDLDGIPGATAGVGFHVYGAPAGPLQITSPTFGSVNTVTSLSVAGTTEQGVSVR
                     LSEYGNTIATVPVSNGSWAASLTFSEGAHQVNAQALDANGNPVNAWSFVEFAVDLSPP
                     QISMEGGDFPFALPLVNTEIAFNVVTARGMANDAVGLRRVIVTVKPIAPASESWTVEA
                     NCGGCQPTLCMQCGNWPAHWYADIELPEPGLYEISATAFDHAGRSTRSNARRFLRFIP
                     AGTPDPFEFLGPPNCLGPLEETPVCFDPFEDGIPFPFGN"
BASE COUNT         1236 a         2464 c         2650 g         1282 t
ORIGIN      
        1 tcagaagcgg cagctgcgtg gtgccgccgg gaaatgcggt gacgcgaaac gtggcgcggc
       61 ccgcgtcgat cacgagctgg gcgagcacgt ccgggacctg gttcccctcg agggtgccgt
      121 cgacgtcgac gggggtcgga accagaagga ccgcgggcgt gaacttttcg acgatgtcac
      181 cgctgggagc gcggtacttg ctgtgaattc gatacgccaa aggcgaaggc accacgccgg
      241 gcggctgcac ggtcggagga gtggtcggca ccagatcgtt gccaccggcc ggcgtctgac
      301 gctcggccgg tgtgggaacg gccggccggt ccacggggag ctccggggcg gcggccgagg
      361 acgcgaagcc gagcgcgacc acgaacggca ctgcggcaag gatcaagccc attcgacgcg
      421 tcttgttgcc tctcacagcc atctccccct cgccgcgaaa cgcttcaggt cacacactcg
      481 atcggatcgg tctcctgaca caccgacggc ggttgcgccg atgcgagcgg gttcaggcga
      541 accaggcccg cactgccggt tcgcgtgacg tacagcttgg cgctcccgtc catcgccacg
      601 gccgacggcg tggccagccc gccgacggtg cgaagatgca ggccccatcg cgtgaactga
      661 tcgacgcgcc cgctcccact ttcggtgacg aagacgccgt actggctggc cagaatgccg
      721 gtgggcagca tcgtgcagcc gtcgccctcg ccctcgccgc cgaacgatcc gaggaagcga
      781 ccgtcaacgg cgaacttttg gattcggttg ttgccggtgt ccgcaacgaa gatctcgtcc
      841 ccccacaccg acacgcctgt gggcgaggag agcttgcccg gctcggcgcc cggcccgccg
      901 atgaccgcga ccggcacccc ggtacgcgag atgatctgaa ccctgtcgtt gccggtgtcc
      961 gcgatcgcca gaagaccctg caacggtccg ttggcgatga acgcgatgcc gcgcggcgag
     1021 gagaactggc cgggcgccgc gccctgaaac ccgaaggttc cgagatacag accggaaaat
     1081 ccccattgtt ggatgcggtg gttgcccgta tcggcgacga agacgatccc gctgtccgag
     1141 atcgcgatgc ctcccggcca tcgcatctga ccctctccgg atccggcaga gccgaagacg
     1201 tcggccgaga acccggcgag atcggtccgc acgatccgat cgtttgcggt gtcggcaacg
     1261 aacagctttc cgaggccgta ggtgacggcc tgcgatgcac cgatcgttcc tgtcagcagc
     1321 gtcccgctcg gcgaatacga aagcgctgca gccggcggcc acagatgctg aatccgcggc
     1381 gccatcgtcg tcgcacactc ggtgccgggc gtggatggca gcgtcggcgc gggcgtcggc
     1441 acgggcgtgg gcaacggagg atcggtggga acgggaggag gcgtcagcgt cgggatcgga
     1501 agcggctcgg tgccggggac ctcgggaacg gccggaaccg gtcccgcagc cgggcgaact
     1561 tgggtggcga aggtgatcag cttgtcgtcg agcaggccga ccgtttccac gatcggcgcg
     1621 aagcgctcga tgtagttccg cgggtacggc gggaccgcgg gcgcgctgaa cgtgaactcg
     1681 aacgcgccga tcgcgccggg ctcgacgcgc ggcgtgatca ttcggattcg tcggccgccc
     1741 gaccacagcc acgtcggatc ctcgcggccg aaggcgctcg cgcgctcccg gggctggacg
     1801 cccacgtcgt tgacggcggc caagcgcacc ggcgtggcgg tgaagttatc ggcatcccac
     1861 gcgaccgaac cggtgttgcg cagccagacg ctcagcactg tcttcgctcc gggatgcatg
     1921 gtcgcgggcg cgggcggaac gaggaccggc tcccacgagt agccggcggg cggagttggg
     1981 agcgccggcg gctgggggac gccggggccc ggcgggatcg ccggcgccgc gccgctcgcc
     2041 ggccgcacga tcgtggtcag gacgatctcg gggcggccct ccatccagcc acccggctcg
     2101 acgagcggaa ggaaccgttc ggcgagcgtg accgggtatg ccggttgctc gggcgcgcgg
     2161 aacgtgaacg tgaacgtgcc gactccaccg ggcggcacga ccggcgtcgt catgcggatc
     2221 cgcttgccgg acgaccagag ccacgagggg tcctgcccgg cgaacgcgct cgagcggtcg
     2281 cgaggcgacg acgtccccag ccgcaccggc gcaggtcccg ccccgtccgc gaaccaggtc
     2341 tgggttccct cgttgcgcag ggcgaccgtc aatgccaccg tcatgccggg atgcatcgtc
     2401 gccggcggct gctgaatcgc gaccggcgtc cagtcgtagg cgggcgtctg cgcttgaacg
     2461 gccggcagcc cggcgacgga cagcgccgcg gcccatgccg cggcgcttgc ccatcgcctc
     2521 atcgctcgcc catcagccga aggcggttca gccgcccgcc agctcgtcga tcgcgttctc
     2581 ggcggacacg accgtcgtgc agttgtgcgc gtgaacggtc gcggccgtgt cgggatcgac
     2641 catgccgatc accggctcga cctgacggtc gatggtctcg gaggccgccc cctcggcagg
     2701 cgtgccgtcg agcgtgttga tgccgtcctc gccgcagccg atctcggtga tgatcacaga
     2761 tccgatcgcc gttccgccgc ggccgtccga gatggagtac gtcagcgtgt cctcgccctt
     2821 gaacgtcggg ctgggcgagt acgtgagtgt tccgtcgggg ttgaccgtgg tggtgccgtg
     2881 cgctccgggc gaggcggaca cgaccgtcaa cacatcgccg tcgacatcgg tgtcgttgcc
     2941 cagaacgtcg aaggtgaccg gcgtgtattg ctccgttgag acgccgtcgc ccaccgcaac
     3001 cggattgtcg ttgacctcgg cgacgtcgat cgacaccgtc gcgatgttcg atgtgtactc
     3061 agcgtcggag acggtgaacg tgaaggcatc gggaccgtag tagttctcga acggcgtgta
     3121 gacgatcgtg ttgccgtcga gcacgtcaag cgttccgtgc gtggggccgt cgacgatcgc
     3181 gaagacgatc tcgtcgccgt cgacgtccga accacccagc acgatgggcg tcgcggtgtc
     3241 ttcgttggtg agaaccgtct gatcgaaggc gatcggcgcg tcgttgaccg gcgtcacgtt
     3301 gatcgacacg gtcgcgacgt tcgagtccag ctcaccgtcg ttgatcttga aggtgaacga
     3361 gtccggaccg ttgtagtcgg cgtccggcgt gtaggtgcag ttcgtgccgg agcactcgat
     3421 cgtgccgtgg gccgggccct cgacgatgga atatgcgagg ttttcgacgt cggtgtcgat
     3481 gtcgaagccg gtcagcacga tctcgaccga gttgtcctcg gccgtctcga ccgagatgtt
     3541 gtcgtcggcg accggcgcat cgttgaccgg cgacacgacg atcgtgaccg tggcctcgac
     3601 cgagttcagc tcgccgtcgt tggcgacgaa ggtaaacgag tcggtgccgt tccagtccgc
     3661 gttcggcgtg tacagcagct cggcgccttc gcccgacaat gagccatagg tcgggccgtt
     3721 tacgaccgag tagctcaagt cctccagctc gttgtccacg tcgctcgcgg tcagcgtgat
     3781 ggcgagcggc gtgtcctcgt cggtcgtgag ctcttgggca tcggcgaccg gagcatcgtt
     3841 gaccgcgttg accgtgatcg acaccgtcgc gatctggctg tccagcagtc cgtcggtgac
     3901 ctggaacgtg aagctgtccg aaccgaagaa gttctcgttc ggcacgtagg tgatggacga
     3961 ggtgccgtcc ggatccgggg agaacgagcc gttctcagga ccggacaaga ccgtgtaggt
     4021 caacgtgtcc aaatccacgt cggtggcctg catcgtgatc tcgagcgggg tgtcctcgtc
     4081 ggtggtcacc gccgaatccg tcgccaccgg agcgtcattg acagggttga cggtgatcga
     4141 gatcagaccg agcggcgaag agtgcagcgc accgtcaaag acgtcgaacg tgacgctgtc
     4201 ggcgccgttg aagtcctgag ccggcgtgta ggtgatcgtc ggcatgtccg tcagatcgag
     4261 cgtaccgttc gatggcaggc cgacgacctc ataaaccaga ttctccggag gggtgtcggt
     4321 gtcggtgccg ctcaccgtga acgtcaccgg cgtgtcctcg tcggtcgtga tcgcctggtt
     4381 gatcgctacc ggcgcatcgt tgaccggcgc gacctcgatc gaaaccgtgc cgcccgtggt
     4441 ggcgaagcca tccgtcgcgg tgtaggtgaa cgaatccggg ccgacgtagt ccagcgaagg
     4501 ggtgtagatc aggttcggcg ccgttcccga caacgttccg ttcgacggcg agccgatcga
     4561 gtagtggagg tcgttgccgt cgatgtccga cgcattcaac gtgatgccga cggccgtgtc
     4621 ctcgttggtg gtgaaggact ggttggcggc gaccggagga cggttgtagt agaagccgaa
     4681 tccccacgcg atgtaggtgt gcttcgcagg gttggttgcg gattggtcgt agtaagcgat
     4741 cgccggcgtc caggtggatc cactgttggt cgagatctcg aacttgtagt tgatcgagaa
     4801 cgatcccacg ccggtcacgg gaaggacggc ggaggcgttg gcggggttcg gcaggccgga
     4861 gaccgacgag gccagcaccc aagcggggcc gctggtcacc gtcgcgagga cgcgcgcgcg
     4921 caccatctgg atcgaagagc ccccgcccga gaagatctga atcccgacgc agttgttcgc
     4981 gcactcccac cgggccgtcg ttcccgagat cgccgcgaac gggatcgtca ccgggctgtt
     5041 cgagcaggtg gggccggggg tgcaaccggg agcgccggcg ctgtcgaggc tcagctgcgg
     5101 aagctgcgac ggcccccagt aggtatgtgt gaacaagccc gggttcgtca ccgtgtagga
     5161 caggttcctg ccgggagtct gaccctcgca gggggtgctg aacccgaacg cgttcgtaca
     5221 cgagttcgac gtcaccgtcg cgccgcctgg cggcgggtag gccggacccg agagcacgcc
     5281 gttgccgaga gccggcgcag gcgcaaccgc taacaccgcg gtcaggacca gaccgagtgt
     5341 cgcgaaagca atgaggcggc gaccgcccaa tgtgttcgac atctgttctc cccctaggcc
     5401 gccccccgcg ggcggttctc cccctatata cggattcgcc acggaacgct tgcgctcctt
     5461 ccgagttgcc cgtttcatgg atccttaaga ggattttcgg gtttgggaga gaaatggctg
     5521 atcacgggcc gtggggggag atcggagagc ccggggggga tcactcgtga agcgtctctg
     5581 catacttgcc ttgcttcttt cgttcgccgc ggttccgagc tcatcccggg cggatggcat
     5641 ggattcgctc aacgagtcat tccgcaccac gttggtgggt ggggggcttg tcagcgaagg
     5701 ggtcgggctg gcgcggcgca acggcggtcc cgcgtcgggc tcgatcacga tcagcgggat
     5761 ccccgaaggg tcctcggtcg ccaaagcctt gctgtactgg agcgtgatcg ccggcgatga
     5821 ccccgtagtc actcttaacg gctccggcgt tttgggaggc cagatcggcc gctcccccga
     5881 gacctgctac ttcaacagcc gcgactcgag ccacgacaat cgcgtgtacc gcgcggacgt
     5941 aacgtctatc gtgaccggca acggcacctt cacggtggcg ggcgtggggg acgtcctgtc
     6001 gcccgccgac ggccagggcg cctcactgct ggttctgtac aagaacgagt ccgcggactt
     6061 ccagcgaacc atcatcatca acgacggcgc ggcgaccgga ggaaaccccg gccagcacta
     6121 cggctcgatc gctcatacgt tcggctcgct gccgactgcg cggccggtgc tcggggcgaa
     6181 gctgcacttc gggttcggcg acgggcagcc cgaatttcct gagggcgcca tgaccgtcgg
     6241 tggccagcaa gtgctggggc cgaactcctt gtcgggcgcc gacggaccgt catgggacga
     6301 cgccgcagtc cccgtgcctg catcgctctt ccccgtcggc acgagcagcg tgctcaaccg
     6361 tctccaggtt gccgacacgt caggcggcga ctgcgtgctc tggacctacg ccgccctggt
     6421 gctcgagcag cagaagtccc tcgtgccggc accgaccatc gtcataacct cgcccgagcc
     6481 cggcacccag cagacctcgc ccgtgacgat cgagggcaca tccacgaacg cgtcggggat
     6541 cgtgttgacc gagcacggac agacgctcgc aacgaccacg ccctcgggct cgggagcctg
     6601 gtcggttagc gtcgcgctgg ccggcggaca tcacgatgtg accgcgaccg cgttggatct
     6661 cgacggcatt ccgggcgcga cggcaggggt aggatttcac gtctacggcg cgccggccgg
     6721 accgcttcag atcacctcgc cgaccttcgg ctcggtgaac acggtgacca gcctgtcggt
     6781 cgccggaacg accgagcagg gcgtgtcggt caggttgtcc gagtacggca acaccatcgc
     6841 gacggtgccg gtgtcgaacg gatcgtgggc ggcgtcgctg accttcagcg aaggcgcgca
     6901 ccaggtcaac gcgcaggcgc tcgacgcgaa cggcaaccct gtgaacgctt ggagcttcgt
     6961 ggagttcgcg gtcgatctct ctccgccgca aatctcgatg gagggaggcg actttccgtt
     7021 cgccctgccg ctcgtcaaca ccgagatcgc attcaacgtt gtaactgcgc gtgggatggc
     7081 gaacgacgcc gtggggctgc ggcgggtcat cgtgacggtg aaaccgatcg caccggcatc
     7141 ggaatcatgg acggtcgagg cgaactgcgg cggatgccag ccgacccttt gcatgcagtg
     7201 cggcaactgg cctgcgcatt ggtacgcgga catcgagctg ccggaacccg gcctgtacga
     7261 gatcagcgcg accgcgttcg atcacgccgg gcggagcacc cgaagcaacg cgcgacgatt
     7321 ccttcggttc ataccggcgg gaaccccgga tccgttcgag ttcctcggcc cgcccaactg
     7381 tctcggcccc ctggaggaga cgccggtctg cttcgatccg ttcgaagacg gcattccgtt
     7441 tccgttcggc aactgatcgc gggtgaccgc ggccgcgcga tagcgcttcg ggaacgacgg
     7501 ttcggctcga tcgggtctta cctggtcagg agcccggtgc agttgtacaa atgatgcgtc
     7561 tggcggcagg cggttgcccc cggcggtcac agcgcatgct tccctatggc ggcctcgcgc
     7621 gccgccgtat cc
//
LOCUS       DATLFO010000145         4478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_45960, whole genome shotgun sequence.
ACCESSION   DATLFO010000145 DATLFO010000000
VERSION     DATLFO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45960"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VM841_05560"
     CDS             <1..500
                     /locus_tag="VM841_05560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL89685.1"
                     /translation="AFPTIKALHPPARLVVGGDGPDREACERAIPGELAGDVEFLGRV
                     TPEAVAAVLGNAAVVALPALGGESFGIVLLEAMAAGRPVVATSIPGYAAVARHEREAL
                     LVPPGDSDALAAAVATVLRDPGLGDALVDAGRRRAAEFDWDRVVEDVEKAYRDAIAAR
                     RHRAD"
     gene            534..1082
                     /locus_tag="VM841_05565"
     CDS             534..1082
                     /locus_tag="VM841_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVL89686.1"
                     /translation="MWVWIVLGVAGLVVLYVIVVFNRLVSLRNRLDNAWSQIDVQLRR
                     RYDLIPNLVHTVKGYATHERTLFEEVTRARSQGLAAGSVHDQEQAENMITAALRQLLA
                     VAEAYPDLKANQNFLALQEELVGTEGKIAYARQFYNDEVQTYNTLGQQFPSSIVAGAF
                     RFRPREFFRTEDATRPPVQVEF"
     gene            1141..2040
                     /locus_tag="VM841_05570"
     CDS             1141..2040
                     /locus_tag="VM841_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVL89687.1"
                     /translation="MFDLIKSNMRRSWLLMAGFVLVVAAFGYLFGILFGGGFIGLVIA
                     IVIAVIMSWSAYLGGDKVVLAVSRARLTTPADEPRLHNLVEGLAIAAGIPKPEVYVVE
                     DPSPNAFATGRDPEHASIAVTRGLLEKMNRLELEGVVAHELAHIANRDTRVLLITATL
                     VGVVVLIADWTLRSFFWGFGGGRRGARSRDGAAVFALVGLVAAVLMPLFASLMQAAVS
                     RRREALADLTGVKYTRYPPGMIAALEKLKADQTIVQSASRATASLWIESPLQRESGFF
                     GKLNHWFDTHPPLDERIRILQEL"
     gene            2132..3025
                     /gene="pdxS"
                     /locus_tag="VM841_05575"
     CDS             2132..3025
                     /gene="pdxS"
                     /locus_tag="VM841_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HVL89688.1"
                     /translation="MSEQVERGTVRVKRGLAEMLKGGVIMDVTNPEQAKIAEDAGACA
                     VMALERVPADIRRDGGVARMADLKIVEAIMESVTIPVMAKARIGHFVEAQILQAAGVD
                     YIDESEVLTPADEAFHIDKWNFTVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGNI
                     VEAVRHLRSILGGIRRIHAAPSEELMTIAKDQGMPFDLVKEIHETGRLPVVMFCAGGV
                     ATPADAALVMQLGAEGVFVGSGIFKSDDPAARAKAIVEATTYFNDPDVLAKVTRTTGE
                     PMRGMDVRSMSDDERLASRGW"
     gene            3030..3602
                     /gene="pdxT"
                     /locus_tag="VM841_05580"
     CDS             3030..3602
                     /gene="pdxT"
                     /locus_tag="VM841_05580"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HVL89689.1"
                     /translation="MTEQVGVLALQGDFEAHQRILRAAGAEAVQVRTAEQLESVDRLV
                     IPGGESTTIGKLAADYGLIDPLRKRAADGMPVFGTCAGLIMCARRLVGGAYDEQPLLG
                     IVDVAVRRNAFGRQVDSFETDLDVKGIDTPVHAVFIRAPVIEQTGEAVEVLAEHGGRP
                     VAARQERTLVCSFHPELTGDDRIHRLFLEL"
     gene            3602..4345
                     /locus_tag="VM841_05585"
     CDS             3602..4345
                     /locus_tag="VM841_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVL89690.1"
                     /translation="MGGHSHWKGIKEKKGKLDKARGKLFAKLLRAIEISARQGDPNPD
                     NNPNLFDAIQRAKDASVPKDNIDRAVARGAGEIAGENYETIFYEGYASGGVAMLVEGL
                     TNNRNRTSQDLRAIFSKHGGSLGEPGSVKWMFDKRGVIVVAKSAADEDKLMELALEAG
                     ADDVRDAGGDWEIVCEQKAFEAVRGAIDAAGIERLSAEVTMLPQNTVAVDGENARKVL
                     RMMDALEDHDDVQAVYANFDIPESVFAEL"
BASE COUNT          715 a         1514 c         1551 g          698 t
ORIGIN      
        1 aggcgtttcc cacgatcaag gcgctgcatc cgccggcccg gctcgtcgtc ggcggggacg
       61 gacccgatcg cgaggcgtgc gagcgcgcga tccccggcga gctcgccggc gacgtcgagt
      121 tcctcggacg cgtcacaccc gaggccgtgg ccgcggtgct cggcaatgcc gcggtcgtcg
      181 cgctgccggc gctgggcggc gagtcgttcg gcatcgtgct gctcgaggcg atggcggccg
      241 ggcgtcccgt cgtcgcgacc tccatcccgg gctacgcggc ggtcgcgcgc catgagcgcg
      301 aggcgctgct ggttccgccg ggggactccg acgcgctcgc cgcggccgtt gcgacggtcc
      361 tgcgcgatcc cgggctcggc gacgcgctgg tggacgccgg ccgccgccgc gcggcggagt
      421 tcgactggga tcgcgtcgtc gaagacgtcg agaaggccta tcgggacgcc atcgcggccc
      481 gccgtcaccg tgcggattga cgcgaccccg ccacgcgcta cgatgcccgc gtgctgtggg
      541 tgtggatcgt ccttggggtc gccggcctcg tcgttctcta cgtcatcgtg gtgttcaacc
      601 ggctcgtgtc gctccgcaac cgcctggaca acgcttggtc ccagatcgac gtgcagctcc
      661 gccgtcgcta cgacctgatc ccgaacctgg tccacaccgt caagggatac gcaacgcacg
      721 agcgcaccct tttcgaggag gtcacgcgag cgcgctcgca aggcctcgcc gccggcagcg
      781 ttcacgacca ggagcaagcc gagaacatga tcaccgcggc cctgcggcag ctcttggccg
      841 tcgccgaggc ctatccggac ttgaaggcga atcagaactt tctcgccctg caagaagagc
      901 tcgtcggaac cgagggaaag atcgcctacg ctcgccagtt ctacaacgac gaggtccaga
      961 cctataacac gctcggccag cagttcccgt cgagcatcgt cgccggggcc ttccggttcc
     1021 ggccccgcga gttcttccgc accgaagacg cgaccaggcc gccggtgcag gtagagttct
     1081 gacccgcccg gcccgctccg gcgccggacg ctccttttcg ccaccgttcg aaggatcacg
     1141 atgttcgacc tgatcaaaag caatatgcgc cgctcctggc ttctaatggc cgggttcgtg
     1201 ctcgtcgtcg ccgcgttcgg ctacctgttc gggattctgt ttggcggcgg cttcatcggc
     1261 ctggtcatcg cgatcgtgat cgccgtgatc atgagctggt cggcctatct gggcggcgac
     1321 aaggtcgtgc tcgcggtctc tcgcgcccgg ctcaccaccc cggccgacga gccccgcctt
     1381 cacaatctgg tcgagggcct ggcgatcgcg gcgggcattc ccaagcccga ggtctacgtg
     1441 gtcgaggatc cctcgccgaa cgcgttcgcg acgggccgcg accccgagca tgcctcgatc
     1501 gcggtcaccc gcggcttgct cgagaagatg aaccggctcg agctggaagg cgtcgtcgcg
     1561 cacgagctcg cgcacatcgc caaccgcgac acgcgggtgc ttctgatcac cgccacgctg
     1621 gtcggcgtgg tcgtgctgat cgcggattgg acgctgcgct cgttcttctg ggggttcggc
     1681 ggcggccggc gcggcgcgcg cagccgggac ggcgcggcgg tgttcgcgtt ggtcggcctg
     1741 gtcgcggcgg tgctgatgcc gctgttcgct tcgttgatgc aggccgccgt gagccggcgg
     1801 cgggaggcgc tcgccgacct gaccggcgtg aaatacacgc gctatccgcc cggcatgatc
     1861 gcggcgctcg agaagctcaa ggcggatcag accatcgtgc agagcgcgag ccgcgcgacc
     1921 gcctcgctgt ggatcgaatc gcccttgcag cgcgaatcgg gcttcttcgg caagctcaac
     1981 cactggttcg acacgcatcc gcccctggac gagcgcatcc gcatccttca ggagctctag
     2041 gacgcggcaa agccgttggc gggccccgat ccccgcggtg taggctgaca taaaaccgca
     2101 ggtagcaggc cgttttcggc ggagggaagc gatgagcgag caggtcgagc ggggtaccgt
     2161 ccgcgtcaag cgcgggctgg ctgagatgct caagggcggc gtcatcatgg acgtcacgaa
     2221 ccccgagcag gcgaagatcg ccgaggacgc gggcgcgtgc gccgttatgg cgctcgagcg
     2281 cgttcccgcc gacatccgcc gcgacggcgg cgtcgctcgc atggccgacc tcaagatcgt
     2341 cgaggcgatc atggagtcgg tcacgatccc ggtgatggcc aaggcccgta tcggtcactt
     2401 cgtcgaggcg cagattctgc aggccgcggg cgtggactac atcgacgagt ccgaggtgct
     2461 gaccccggcc gacgaggcgt tccacatcga caagtggaac ttcaccgttc cgttcgtgtg
     2521 cggcgcgacc aacctcgggg aggcgctgcg ccggatcggc gaaggcgcgg cgatgatccg
     2581 ctcgaaaggc gaggcgggga ccggcaatat tgtggaggcc gtccgccacc tgcgctcgat
     2641 cctcggcggc atccggcgca ttcacgccgc gccgtccgaa gagctcatga ccatcgccaa
     2701 ggaccagggc atgccgttcg atctggtgaa ggagatccac gagaccggcc ggctgccggt
     2761 tgtgatgttc tgcgccggcg gcgtggcgac gcccgccgac gccgcgctgg tgatgcagct
     2821 cggcgccgaa ggcgtgttcg tcggctcggg cattttcaaa agcgacgacc cggccgcacg
     2881 cgccaaggcg atcgtcgagg cgaccaccta cttcaacgac cccgacgtgc tggccaaggt
     2941 cacgcgcacg accggcgagc cgatgcgcgg catggacgtg cgttcgatga gcgacgacga
     3001 gcgtctggcg tcgcgcggct ggtaaggccg tgaccgagca ggtcggcgtc cttgctctcc
     3061 aaggagactt cgaagcgcac cagcggatcc ttcgtgccgc cggcgccgag gccgttcagg
     3121 tgcgcacggc cgagcagctc gagtcggtcg atcgtctggt gatcccgggc ggcgagtcca
     3181 ccaccatcgg caagctcgcc gccgactacg gactgatcga cccgctgcgc aagcgcgcgg
     3241 cggacggcat gccggtcttc ggaacgtgcg cgggcctgat catgtgcgcg cggcgtctgg
     3301 tcggcggcgc ctacgacgag cagccgctgc tgggcatcgt ggacgtcgcg gtgcgacgca
     3361 atgcattcgg acgccaggtg gattccttcg agaccgacct cgacgtcaag gggatcgaca
     3421 cgcccgtgca cgcggtgttc atccgcgcgc cggtcatcga gcagaccggc gaagccgtcg
     3481 aggtgctcgc cgagcacggc gggcggccgg tcgcggcccg gcaggaacgc accctggtat
     3541 gttcgttcca tccggagctg accggcgacg accgcattca ccgacttttc ttggagctgt
     3601 aatgggcggg cattctcatt ggaagggcat caaggagaag aagggcaagc tcgacaaggc
     3661 ccgcggcaag ctgttcgcca agctgctgcg cgccatcgag atctcggccc gccagggcga
     3721 tcccaatccc gacaacaacc cgaacctgtt cgacgcgatc cagcgagcga aggacgcgtc
     3781 ggtccccaag gacaacatcg accgcgccgt cgcgcgcggc gcgggtgaga tcgcgggcga
     3841 gaactacgag accatcttct acgaaggcta cgcgtcgggc ggggtcgcga tgctcgtcga
     3901 agggctgacg aacaaccgca accgcacctc gcaggatctg cgcgcgatct tctccaagca
     3961 cggcggcagc ctgggtgagc cgggcagcgt gaagtggatg ttcgacaagc ggggcgtgat
     4021 tgtggtggcc aagagcgccg ccgacgagga caagctgatg gaactcgccc tggaggcggg
     4081 cgccgacgac gtgcgtgacg caggcggcga ttgggagatc gtgtgcgagc agaaggcctt
     4141 cgaggcggtt cgcggcgcga tcgatgcggc cgggatcgag cggctcagcg ccgaggtcac
     4201 catgcttccg cagaacaccg tcgccgtcga cggcgaaaac gccaggaaag tccttcgcat
     4261 gatggacgcg ctcgaggacc acgacgacgt ccaggccgtc tacgccaact tcgacatccc
     4321 cgaatcggtc ttcgccgagc tctagcttcg tccccggcag gaacacggct cgggcgaagc
     4381 gaacaatccg accatgaaga cgcttcgcgt cgtcgcggcc ctcgccgtgc ccttgatgac
     4441 ggccgcgccc gggcacgccg cggtccagga cgtcaccg
//
LOCUS       DATLFO010000146         7962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_104656, whole genome shotgun sequence.
ACCESSION   DATLFO010000146 DATLFO010000000
VERSION     DATLFO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104656"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VM841_05590"
     CDS             complement(<1..262)
                     /locus_tag="VM841_05590"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HVL89691.1"
                     /translation="MSASRSSAEIVAELLRALGEDPSRPGLADTPERVAAAYAELLSG
                     RDVDPASILEPLPGEHGSGLILARDIRLASLCEHHLLPFVGTA"
     gene            complement(297..536)
                     /locus_tag="VM841_05595"
     CDS             complement(297..536)
                     /locus_tag="VM841_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89692.1"
                     /translation="MFGQEEFARTADTVLCQAEDDSLTFVARGDAGRSQDCVWLGCDE
                     TYAIAVRPGQRYVLRIFNYLNAGPVEVTTSYARNR"
     gene            complement(549..2561)
                     /gene="ftsH"
                     /locus_tag="VM841_05600"
     CDS             complement(549..2561)
                     /gene="ftsH"
                     /locus_tag="VM841_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVL89693.1"
                     /translation="MPPNLPGRNSGSSQRPGSPFNRVFRGPIVYLALIALLLWTFVSY
                     FGPNRTVKELRLDQFETSLDAGQVETATFFDRDARVEGKLTDGTRYRVKYPDQYVDTL
                     LKNVREHPEVQVKIESGGQSPILSFIFQLLPFLLIFGFFFFFMNQMQGGGNRVMSFGK
                     AKAKLVTKDMPKVTFKDVAGVDEAVEELTEVKEFLQNPAKFQSLGAKIPKGVLLYGPP
                     GSGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGAARVRDLFEQAKTNAPAIIFVDE
                     IDAVGRQRGAGVGGGHDEREQTLNQLLVEMDGFDVKTGVIMIAGTNRPDILDPALLRP
                     GRFDRQIVVDRPDLKGRLAILEVHARGKPVADDVDLEVLARRTPGFTGADLANVMNEG
                     ALLAARGGEKKITMHVLEESIDRVLAGPERKSRVISEKEKRVIAYHEAGHAVVGHALP
                     NADPVHKISIIPRGRALGYTLSLPTEDRFLVTRAELIDELAMFLGGRVAEELVFEDPT
                     TGAQDDISKATKIARQMVTEYGMSDRLGPLTLGQKSDNVFLGRDMHSTPDYSDNIAFE
                     IDKEVRRLIDEAHQEALDILNENRKVLDNIVDTLFAKETIEKEELAKILGPIKKRPAR
                     PFDGRTAMKANGGSKRRPAAEAPAPAGKPSVKRKPKPKPKPGLSPA"
     gene            complement(2625..2894)
                     /locus_tag="VM841_05605"
     CDS             complement(2625..2894)
                     /locus_tag="VM841_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89694.1"
                     /translation="MARTFRATISFSAAGGTPVARVPIAPEIAEELGLRPGESLRASM
                     RGAEFTGTVSGSLKTPSLSVPMDVITTLGIREGQGVRMTVIGRAE"
     gene            complement(2912..3499)
                     /gene="hpt"
                     /locus_tag="VM841_05610"
     CDS             complement(2912..3499)
                     /gene="hpt"
                     /locus_tag="VM841_05610"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HVL89695.1"
                     /translation="MGAGLAWRTEEVVVGEINTDIEQVLITEDEIQSKVALLGKEISE
                     DYRGKDLLLVGVLKGAFTVMADLSRAISIPCEFDFMAVSSYGSSTQTSGIVRIMKDLD
                     LEIAGRHVLIVEDVIDSGLTLSYLLRNLRSRGPASLEVCAMLSKPDAQKVKLDVKYHG
                     FELPPVFVVGYGLDYAERYRNLPYVGTLKPHIYQD"
     gene            complement(3484..4830)
                     /gene="tilS"
                     /locus_tag="VM841_05615"
     CDS             complement(3484..4830)
                     /gene="tilS"
                     /locus_tag="VM841_05615"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVL89696.1"
                     /translation="MKPDAFLAAVGDTMRRRGMDAPAVILVAVSGGPDSLALVHALHR
                     LEYPVEIAHFDHGVREGSGDDARFVHEAAEELDVRFHLGRPDDHTVVRRHGESPEEAL
                     RRERLAFLEQTAQRHGIRRIATGHTLDDQAETVLMRMLTGAGRRGLSGIPPVRGPYIR
                     PLIDLRRADTAAFCKALKLKPRRDPTNGDERYLRNAIRADLLPHLAARYNARIAEALA
                     RSADVLRDEDALLDAMLADAIRPEAAAGALRIRTEDLAELHPAMQRRAIRRLAALDAV
                     ATERLRLLALHGRSGDQLFLPGRLNARLEYGWLVVGPATSSPARPDAETLIVPGVTDL
                     PGWAARMRAVVAASRPDPFPDGIAACALDANAAGEPLSVRAPKRGDRFRPLGMARSKA
                     LSDVFTDAKVPRGERATTPVVDGPNGIVWVVGHRFDDRCKVTQTTRRVLVLEWEQA"
     gene            complement(4827..5900)
                     /locus_tag="VM841_05620"
     CDS             complement(4827..5900)
                     /locus_tag="VM841_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HVL89697.1"
                     /translation="MPSTPELVDWSVARRVGRAIAGNGPPVARSEAERIVKEFHGHVE
                     TADRLVRAYTNLEPMREPGDTAVLGRAAWVDVNVEAMRGLLRPLGERLAPKIGNRRSG
                     RLAVGLQIGILLGYISQKVLGQYDLLLATGEPGRVYFVGPNVVAAERRNRLDPHDFRL
                     WIALHEVTHRTQFLAVPWLRDRVADLIEQYLAGIKVDRERLKEAMARVKELATAGPDA
                     WRRANIIEIFLTGEQREAIDRMQSLMSVVEGHGNFVMDGVAAEEIPTYQNLREALNRQ
                     RAETGGAERAFQKLIALDLKYEQYAVGQQFFDEVAAEGGMAAVNRVWDKPENLPTLDE
                     LRDAKSWLQRVTPEPTLFDEQGS"
     gene            complement(5970..6281)
                     /locus_tag="VM841_05625"
     CDS             complement(5970..6281)
                     /locus_tag="VM841_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89698.1"
                     /translation="MKPHRSITRALTVLVSALVFSLAVALPAFSADSSPIAPTPTQTA
                     EGAEGEGEAGGTPVIAEPGPPASYYASYGLIAIGGLTVLAVLAGYLFQAPGFRRSSKG
                     A"
     gene            6398..6667
                     /locus_tag="VM841_05630"
     CDS             6398..6667
                     /locus_tag="VM841_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVL89699.1"
                     /translation="MDDDRYTWQEESACRGVEPEVFFPVSEDDAWRAKEICGVCTVKA
                     QCLVFSLQNRERYGVWGGVTEKERIDMQRRGVAQRILAGTLQHAG"
     gene            6864..7877
                     /locus_tag="VM841_05635"
     CDS             6864..7877
                     /locus_tag="VM841_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89700.1"
                     /translation="MRARLAFAAALVTLLTATAAPAGTAHVPAVDLPAVGLPASPTDT
                     VVENMPARAADPVRRATTPLTPIPYDFSCEIEETLDGVTRRYSTGAAGELGDEPVTFE
                     ADTAFVRENDRELERSYSCTIEIGRGMERDIANGPAVGAVIARVNTVGHGHSIVVDEQ
                     GRTIGVAGIRGGRRTLVSGERIVWTMQAGTDGSTWNVGRVHAASCFGLTLARATNAPL
                     DAWCSHEEPDFGPPSQPKPEPKPHDKTFTGQVESVVRGGTPDPVEAEADRIVEMSGQI
                     VDDVIPAKPSSPMPAPPEVPGAAMPPVTPVTPVTAVTGVTAVTAVLPTGGTDLSRRGD
                     LNS"
     gene            complement(7851..7922)
                     /locus_tag="VM841_05640"
     tRNA            complement(7851..7922)
                     /locus_tag="VM841_05640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7888..7890),aa:Thr,seq:cgt)
BASE COUNT         1293 a         2627 c         2759 g         1283 t
ORIGIN      
        1 cagcggtgcc gacgaacggc agcagatgat gctcgcacag cgaggcgagc cgaatgtcgc
       61 gagccaggat caaacccgag ccgtgctcac cgggcagcgg ctccaggatc gaagcaggat
      121 cgacgtcgcg gccggacaaa agctcggcat acgccgctgc gacccgctcc ggcgtatccg
      181 ccagccccgg ccgcgacgga tcctcgccga gcgcgcgcag cagctcggca acgatctcgg
      241 cgctcgaacg ggatgcactc atcctcgtca ttgtacggcg gcgccgcgct ccccgatcag
      301 cggttgcgcg catacgacgt ggtcacctcg accgggccgg cattcagata gttgaaaatc
      361 cgcaggacgt agcgctggcc gggccgaacc gcgatcgcat aggtctcgtc gcagcccagc
      421 cacacgcaat cttgtgacct gccggcgtcc ccccgggcga cgaaggtcaa cgagtcgtct
      481 tcggcctggc acaacacggt gtcggcggtt cgggcgaatt cctcctgccc gaacattaaa
      541 agccggtgct atgccggcga gaggccgggc ttgggcttcg gcttgggctt gcgcttgacg
      601 ctgggcttgc cggcaggagc cggcgcctcg gcggcgggac ggcgcttgga gccgccgttg
      661 gccttcatgg cggtgcgccc gtcgaacgga cgggccggac gcttcttgat cggaccgagg
      721 atcttggcca gctcttcctt ctcgatcgtc tctttcgcga acagcgtgtc gacgatgttg
      781 tcgaggacct tgcggttctc gttcaggatg tcgagcgctt cctggtgcgc ctcgtcgatc
      841 aggcggcgca cctccttgtc gatctcgaag gcgatgttgt cggagtagtc gggtgtcgaa
      901 tgcatgtcgc ggcccaggaa cacgttgtcg ctcttctggc cgagcgtgag cgggccgagc
      961 cggtcgctca tgccgtactc ggtgaccatc tgacgcgcga tcttggtggc cttgctgatg
     1021 tcgtcctgcg cgccggtggt cgggtcttcg aaaaccagct cttcggcgac gcggccgccc
     1081 aggaacatcg ccagctcgtc gatcagctcg gcgcgggtca ccaggaagcg gtcttcggtc
     1141 ggcagcgaca gcgtgtagcc aagcgcgcgg ccgcgcggga tgatcgagat cttgtggacc
     1201 gggtcggcgt tgggcagcgc gtgcccgacg acggcgtgac cggcctcgtg gtaggcgatg
     1261 acgcgcttct ccttctcgga gatcacgcgg ctcttgcgct cgggaccggc caggacgcgg
     1321 tcgatcgact cttcgagcac gtgcatggtg atcttcttct ccccgccgcg cgcggcgaga
     1381 agcgcgccct cgttcatgac gttggcgagg tccgcgcccg tgaagccggg ggtgcggcgt
     1441 gcgagcactt ccaggtccac gtcgtcggcg accggtttgc cgcgcgcgtg aacctcgagg
     1501 atcgcgaggc ggcccttcag gtcggggcgg tccaccacga tctgacggtc gaagcggccg
     1561 ggacgaagca gcgccgggtc caagatgtcg ggccggttgg tgccggcgat catgatcacg
     1621 ccggtcttga cgtcgaagcc gtccatctcg accagcagct ggttgagcgt ctgctcgcgc
     1681 tcgtcgtggc cgccgcccac gccggcgccg cgctggcggc cgaccgcgtc gatctcgtca
     1741 acgaagatga tcgcgggcgc gttggtcttg gcctgctcga acaggtcacg gacgcgggcc
     1801 gcacccacac cgacgaacat ctcgacgaag tccgagcccg agatcgaata gaacggcacg
     1861 cccgcctcgc ccgcgacggc gcgggccagc agcgtcttgc ccgatcccgg cgggccgtac
     1921 agcagcacgc ccttggggat cttggcgccc agcgactgga acttggccgg gttctggagg
     1981 aactccttga cctcggtgag ctcctcgacg gcctcgtcca cgccggctac gtccttgaac
     2041 gtgaccttgg gcatgtcctt ggtgacgagc ttcgccttgg ccttgccgaa cgacatcact
     2101 cgattcccgc cgccctgcat ctggttcatg aagaagaaga agaacccgaa gatcaataag
     2161 aagggcaaga gctgaaagat gaacgacagg atcggcgact ggccgcccga ctcgatcttg
     2221 acctggacct cggggtgctc gcgcacgttc ttcaagagcg tgtcgacgta ctggtcgggg
     2281 tacttcaccc gatagcgggt gccgtcggtc agtttgcctt cgacccgggc gtcgcggtcg
     2341 aagaaggtag ccgtctcgac ctgaccggcg tccagcgagg tctcaaactg atcgagccgc
     2401 aactctttga cggttcggtt cggcccgaaa taggacacga acgtccagag cagaagcgcg
     2461 atgagcgcga gatagacgat cggtccgcgg aagaccctat tgaatggtga tccgggacgc
     2521 tggctgctgc cgctgttgcg gccgggaagg ttcggtggca taggctcggc cgatcgtacc
     2581 accccggccg cattcggcgc agggagtcac gaggtcgggg tcgtttactc ggctcgtccg
     2641 atcaccgtca tccggacacc ctggccttct cgaattccca aagtggtgat gacgtccatc
     2701 gggacggaca ggctcggggt cttgagcgat cccgagacgg tgccggtgaa ctcggccccg
     2761 cgcatcgagg ctcgcaggct ctcgccgggc cgcaggccca gctcttcggc gatctcgggc
     2821 gcaatcggaa cccgcgcgac cggggtccct ccggcggccg agaacgagat cgtcgcccgg
     2881 aacgtccgtg ccacaagcct ctccttgttg gttagtcctg gtagatgtgc ggcttgagtg
     2941 taccgacgta gggcagattc cggtatcgct cggcatagtc caagccgtag ccgaccacga
     3001 agaccggggg aagctcgaag ccgtggtact tcacgtcgag cttaaccttc tgggcgtccg
     3061 gcttggaaag catcgcgcag acctcgagcg aggccggccc gcgcgaccgc agatttcgga
     3121 gcaagtacga cagggtcagg cccgagtcga tgacgtcctc gacgatcaga acgtggcggc
     3181 cggcgatctc gaggtcgagg tccttcatga tgcgcacgat gccgctggtc tgggtcgacg
     3241 agccatagga cgagacggcc atgaagtcga actcgcaggg aatcgagatc gcgcgggaca
     3301 ggtcggccat gacggtgaag gcgcccttga gcaccccgac cagcagcaga tccttgcccc
     3361 ggtagtcctc ggagatctcc ttgccgagca gcgcgacttt ggactggatc tcgtcttcgg
     3421 tgatcagaac ctgctcgatg tcggtgttga tctcgcccac aacgacttcc tccgttcgcc
     3481 aggctaggcc tgctcccatt ccagcaccag aacccgccga gtcgtctgcg tgaccttgca
     3541 tcgatcgtcg aagcggtgcc cgacgaccca gacgatcccg ttcggtccgt ccacgaccgg
     3601 tgtcgtcgca cgctcacccc gcggaacctt cgcgtcggtg aacacgtcgc tcagcgcctt
     3661 cgatcgtgcc atgcccaacg gccggaaccg gtcgccgcgc ttgggcgccc ggaccgacag
     3721 cggctcgccg gcagcgttcg cgtccagcgc gcacgccgcg atgccgtcgg ggaagggatc
     3781 cggccgcgag gccgccacga ccgcacgcat ccgcgccgcc catcccggca gatcggtgac
     3841 tccgggaacg atgagcgttt ctgcgtccgg ccgggccggc gaggaagtgg ccggcccgac
     3901 gacgagccaa ccatattcga gacgcgcgtt caatcgaccg ggcaagaaaa gttggtcgcc
     3961 ggagcgaccg tggagcgcaa gcaagcgaag ccgttcggtt gcgacggcgt cgagcgccgc
     4021 gaggcggcgg atcgcgcggc gctgcatcgc gggatgaagc tcggccaggt cctcggtgcg
     4081 gatccgcagc gcgccggccg cggcctcggg ccggatcgcg tcggccagca tcgcgtccag
     4141 cagcgcgtcc tcgtcgcgaa ggacgtctgc gctgcgcgcg agcgcctcgg cgatgcgcgc
     4201 gttgtagcgc gcggccagat gcggcaacag gtcggcgcga atcgcgttgc gcaggtagcg
     4261 ctcgtcgccg ttggtcgggt cgcggcgggg cttgagcttg agcgccttgc agaacgccgc
     4321 ggtgtcggcg cggcgaaggt cgatcagcgg gcggatgtag gggccgcgaa ccggcgggat
     4381 gcccgacagg ccgcgccggc ccgcgccggt gagcatgcgc atcaagacgg tctctgcctg
     4441 atcgtcgagc gtatgaccgg tggcgatgcg gcggatgccg tgccgctgcg ccgtctgctc
     4501 gaggaacgcg agccgttcgc ggcgcagtgc ctcctcgggg ctctccccgt ggcgccgcac
     4561 caccgtgtga tcgtcgggac gacccaagtg gaagcgcacg tcgagctcct cggcggcctc
     4621 gtgcacgaag cgggcgtcgt cgccggagcc ctcgcgaaca ccgtgatcga agtgggcgat
     4681 ctcgacgggg tactccagcc ggtgcagcgc gtgcacgagc gcgagcgagt ccggaccgcc
     4741 cgagacggcg acgaggatga ccgcgggggc gtccatcccg cggcggcgca tggtgtcacc
     4801 gaccgcagcg aggaacgcat ccggcttcac gagccttgtt cgtcgaacag cgtgggctcg
     4861 ggcgtaaccc gctgcagcca cgatttcgcg tcacgaagct cgtcgagcgt cggcaggttc
     4921 tcgggcttgt cccagacgcg gttcacggcg gccatgccgc cttccgcggc aacctcgtcg
     4981 aagaactgct gcccgaccgc gtactgctcg tacttgaggt cgagcgcgat cagcttctgg
     5041 aatgcgcgct cggcaccgcc ggtctcggcc cgctgccggt tgagcgcctc gcgaaggttc
     5101 tggtaggtgg gaatctcctc ggcggcgacg ccgtccatca cgaagttgcc gtggccttcc
     5161 acgaccgaca tcagcgattg catccggtcg atggcctcgc gctgctcgcc ggtgaggaag
     5221 atctcgatga tgttggcgcg gcgccacgcg tccggccccg cggtcgcgag ctccttgacg
     5281 cgcgccatgg cctccttgag ccgctcgcga tccaccttga tcccggccag gtactgctcg
     5341 atcagatcgg caacgcggtc gcgcaaccac ggcacggcca ggaactgcgt gcggtgtgtg
     5401 acctcgtgaa gcgcgatcca cagccggaag tcgtgcggat cgagccggtt gcggcgctcc
     5461 gccgcgacga cgttagggcc gacgaagtag acgcggccgg gctcgccggt ggccagcagc
     5521 aggtcgtact ggcccaggac cttctgcgag atgtagccga gaaggatgcc gatctgcagg
     5581 ccgaccgcga ggcgcccgct ccggcggttg ccgatcttgg gggcgagacg ctcccccaga
     5641 ggacgcagca ggccgcgcat cgcctcgacg ttgacgtcca cccacgcggc gcggccgagc
     5701 actgcggtgt cgccgggctc acgcatgggc tcgagattcg tatacgcgcg gacgagacgg
     5761 tcggcggtct cgacatggcc gtggaactcc ttgacgatgc gttcggcttc ggaccgggcg
     5821 acgggcgggc cgttgccggc gatcgcgcgc ccgacgcggc gggcgaccga ccagtcgacg
     5881 agctcaggag ttgaaggcat caacgcgagg atatcccctc ggaggggatt cgaaccgcgg
     5941 atgccggatt tcgcgagacg cgaagcggac tatgcgcctt tggagctacg gcggaagccg
     6001 ggtgcctgga acagatatcc ggcgagaacg gcgaggacgg tcaagccgcc gatggcgatc
     6061 aatccgtagg acgcgtagta cgaagccggc gggccgggct cggcgatgac cggcgtgccg
     6121 cctgcctcgc cttcgccttc cgccccttcg gccgtttggg tcggcgtcgg ggcaatcggc
     6181 gatgaatcag cgctaaacgc cggcaatgcg acggcgaggc tgaaaaccaa ggcagacacg
     6241 agcacggtca gtgcgcgcgt gatcgaacgg tgtggtttca aagcggcgga atgctaccac
     6301 acacgcaccc gagaaccacc aaacttctgg ggtgcgggtg tttactttcc gatgaggact
     6361 gctaaactct tgcaagcgct agtgaaagga ttcacaagtg gacgacgacc ggtacacctg
     6421 gcaggaggaa tctgcctgcc gcggcgtcga gccggaggtc ttcttccctg tctccgagga
     6481 cgacgcgtgg cgcgccaagg agatctgtgg agtctgcacc gtcaaagctc aatgcctcgt
     6541 attctcgttg cagaaccgcg agcgctacgg agtttggggc ggtgtgaccg agaaggagcg
     6601 catcgacatg cagcgtcgcg gtgtcgcgca gcgcatcctc gccggaaccc tccagcacgc
     6661 cggatagcga acctgtaagt cgccggcaac gatgccggcg cacgggcggg cccgtctccc
     6721 cgccccggcc cggggccacc gtactttcgt tccccgacgg ctcccctcat agattcgaat
     6781 cagttgattt ccccctcctc ccgacacgaa tggggcgaat gcgccatccg gggggaggcg
     6841 cacagagcga cagggggaac atcatgcgag cccgcctcgc cttcgccgca gcgctggtca
     6901 cgctgctgac cgcgaccgcc gcacctgccg gcacggccca cgtgcccgcc gtcgaccttc
     6961 ccgccgttgg tttgccggcg agtcccaccg acaccgtcgt cgagaacatg ccggcgcgcg
     7021 ccgccgatcc ggtccgccgc gccaccacgc cgctgacccc gatcccctac gacttctcgt
     7081 gcgagatcga ggagacgctc gacggcgtca cgcgccggta ctccacgggc gcggccgggg
     7141 agctgggtga cgagccggtc acgttcgagg ccgacaccgc cttcgttcgc gagaacgacc
     7201 gggagctgga gcgctcctac tcgtgcacga tcgagatcgg ccgcggaatg gagcgcgaca
     7261 ttgccaacgg gccggccgtt ggggcggtca tcgctcgtgt gaacacggtc gggcacggcc
     7321 attcgatcgt ggtggacgag cagggccgaa cgatcggggt cgccgggatc cggggcggac
     7381 gccgcacgct ggtgtcgggc gagcgcatcg tttggacgat gcaagccggg acggacggct
     7441 cgacgtggaa cgtgggaaga gttcatgcgg cgtcgtgttt cgggctcacg ctggcgcggg
     7501 ccacgaacgc gccgttggat gcgtggtgca gccacgaaga gcccgacttc ggcccgccgt
     7561 cgcagccaaa gcccgagccg aagcctcacg ataagacctt cacgggacag gtcgaatccg
     7621 tcgttcgcgg gggcacgccg gacccggtcg aagccgaagc ggaccggatc gtggagatgt
     7681 ccggacagat cgtcgacgac gtcatcccgg cgaagccgtc gagcccgatg cctgcgccgc
     7741 cggaggttcc cggggccgcg atgccgcccg tgacgcccgt gacgcccgtg acggcggtga
     7801 cgggggtgac ggcggtgacg gcggtgcttc cgaccggggg aacggatttg agccggagag
     7861 gagatttgaa ctcctgacct gccgcttacg aggcggctgc tctgcccctg agctactccg
     7921 gcgagctgcc gtcgggctga tctgacggcg cgggcgattc ta
//
LOCUS       DATLFO010000147         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_55243, whole genome shotgun sequence.
ACCESSION   DATLFO010000147 DATLFO010000000
VERSION     DATLFO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55243"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VM841_05645"
     CDS             <1..705
                     /locus_tag="VM841_05645"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018806232.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVL89701.1"
                     /translation="GNNLRGEAVAGGYADAFAYPGFVPAYIRPLFSEGKGPFRWVALS
                     GEESDIVATDRAIAALFPDNDPLQRWLRMAAERVAFQGLPARICWLGYGERDQAGLRF
                     NEMVARGDLAAPIVIGRDHLDSGSVASPYRETEAMRDGSDAIADWPILNAMLNVASGA
                     TWVSVHHGGGVGMGKSIHAGVVVVADGSADGALRLERVLTNDPGTGVMRHADAGYPEA
                     LDVARERGVHIPMPPA"
     gene            702..1898
                     /gene="hutI"
                     /locus_tag="VM841_05650"
     CDS             702..1898
                     /gene="hutI"
                     /locus_tag="VM841_05650"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HVL89702.1"
                     /translation="MSGAADFVLRGAGRLVTCDPERDGVLGVIERGALAAREGVVVWC
                     GPESDLGTVEVAADATELDARGAAVVPGLVDCHTHLVFAGDRLAEFAARMRGEAYTAG
                     GVRHTKAATRAASTETLRELARRRLDRFLSFGVTTIEAKSGYGLNAEHERRLLEVAAT
                     AGHTVEVVRTFMGAHVVPDEYADDPDGYVALVAEGMIPGIGSLAEFCDVWIDEGAFTP
                     AQARIILRAARSEGLSLKVHAEQLARTGGAAVAAEFGAVSADHLEHATEDDAAALARA
                     RTVAVLLPGASMMTQSSPAPARMLIDAGVRVAISTDFNPGSSYSENLPLAASLACAQL
                     GMTVEEAILGVTRHAAAALARDRTHGVLRPGSRADLVVLDASHEAELVAHYGTPAIHA
                     VIKGAA"
     gene            complement(1895..2293)
                     /locus_tag="VM841_05655"
     CDS             complement(1895..2293)
                     /locus_tag="VM841_05655"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HVL89703.1"
                     /translation="MKIGFRSLPGPAEDVRRPLVDVKVGGLDDAALACLLDTGSLHNR
                     FAAWVAREAGIDLDGIQPHSIGVGGRPVIARTTVVSLAVGSHRWEAPVAFCEPWPWDF
                     QLLGQQGFLRFFRVVLDAADYRMEITPNRR"
     gene            complement(2290..2766)
                     /locus_tag="VM841_05660"
     CDS             complement(2290..2766)
                     /locus_tag="VM841_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89704.1"
                     /translation="MKKETVVVAGHSFTLSRERIEAICRDLYPDPVRDHYTVIEGRRF
                     PPKQIIGAITGLDRNDFTTNQARSILRRLGFASGRAGESKPPRAAESAPAYRHSDADA
                     LRPYLGRWVATRAGQVVIAADSPQAVLAGLRAAGIKAESLFRVPLDPAADVGGFAS"
     gene            2838..>3153
                     /locus_tag="VM841_05665"
     CDS             2838..>3153
                     /locus_tag="VM841_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HVL89705.1"
                     /translation="MLVIVAPATFKGSLAATDAAEAIAAGVRAAGGTPVLLPVADGGE
                     GTLDALIAGAGGTIMGVIARGPLGLPVRGHLGRLTDGTGVVEMAQASGLMLVAAHERQ
                     PMR"
BASE COUNT          455 a         1085 c         1157 g          456 t
ORIGIN      
        1 ggcaacaacc tgcgcggcga ggccgtcgcc ggcggctacg cggatgcgtt cgcctatccg
       61 ggcttcgtgc ccgcctacat ccggccgctg ttctccgaag gcaaaggtcc gttccggtgg
      121 gtcgcgctgt cgggcgagga atccgacatc gtcgccaccg accgagcgat cgctgcgctg
      181 tttcccgaca acgatccgct gcagcgatgg ctgcgcatgg cggcggagcg ggtcgcgttc
      241 caaggtctgc ccgcgcgcat ctgctggctc ggctacggcg agcgcgatca ggccggcctt
      301 cgcttcaatg agatggtcgc gcgcggcgac ctcgccgcgc cgatcgtgat cggccgcgac
      361 catctggact ccggcagcgt cgcctcgccc taccgcgaga ccgaggcgat gcgcgacggc
      421 tccgacgcca tcgccgactg gccgatcctg aacgcgatgc tcaatgtcgc ctcgggagcg
      481 acgtgggtca gcgtgcatca cggcggtggc gtcggcatgg gcaagtccat ccatgccgga
      541 gtcgtcgtgg ttgccgacgg ttccgccgat ggcgcgctgc ggctggagcg cgttctgacc
      601 aacgatccgg ggacgggcgt gatgcgccat gcggacgcgg gttatcccga ggcgctcgac
      661 gtcgcgcgcg aacgcggcgt gcacatcccg atgccgccgg catgagcggt gccgcggact
      721 tcgtcctgcg gggtgccggg cgcctggtca cctgcgatcc cgagcgcgac ggtgtgctgg
      781 gcgtgatcga gcgcggcgcg ctcgccgcgc gcgaaggcgt ggtcgtgtgg tgcggcccgg
      841 aaagcgatct ggggacggtc gaggtcgcgg cggatgcgac cgagctggac gcgcgcggcg
      901 cggccgtcgt gcccggcctg gtggactgcc atacgcatct ggtgttcgcc ggcgaccggc
      961 tggccgagtt cgcggcgcgc atgcgcggcg aggcctatac cgccggcggt gtccggcaca
     1021 ccaaggccgc gacgcgcgcc gcgtccaccg agacgctgcg cgaactggct cgcaggcggc
     1081 tggaccggtt cttgtcgttc ggggtcacca cgatcgaggc caagagcggc tacgggctga
     1141 acgccgagca cgaacggcgg ctgctggaag tggccgcgac ggccggtcac accgtcgaag
     1201 tggtccgcac gttcatgggc gcgcacgtgg ttcccgacga gtacgccgac gatcccgacg
     1261 gctacgtcgc attggtcgcc gaaggcatga tccccgggat cggctcgctc gccgagttct
     1321 gcgacgtgtg gatcgacgag ggcgcgttca cgcccgccca ggcgcgcatc atccttcgcg
     1381 ccgcgcgcag cgaaggcctg tcgctgaagg ttcacgccga gcagctcgca cgcacgggcg
     1441 gcgcggcggt cgccgccgag ttcggcgccg tcagcgccga ccatctagag cacgctaccg
     1501 aagacgacgc cgccgcgctg gcacgcgccc gcaccgtggc ggtgctgctt ccgggcgcgt
     1561 cgatgatgac gcaaagctcg cccgcgccgg cccgcatgct gatcgacgcg ggcgtgcgcg
     1621 tcgcgatctc gaccgacttc aatcccggct cgtcctacag cgagaatttg ccgctggccg
     1681 cctcgctcgc ctgcgcgcag ctgggcatga ccgtggaaga ggcgatcctg ggcgtgacgc
     1741 gccatgcggc cgcggcgctc gcgcgcgacc ggacgcacgg cgtgctgagg cccggctcgc
     1801 gggccgattt ggtggtactg gacgcttcgc acgaagccga gctggtcgcg cactacggaa
     1861 cgccggcgat ccacgcggtg atcaagggcg ccgcttagcg gcgattcggc gtgatctcca
     1921 tccgatagtc ggccgcgtcg agcacgacgc ggaagaagcg caggaatccc tgctggccga
     1981 gcagctgaaa gtcccacggc cacggttcgc agaaggccac cggcgcctcc caacggtgcg
     2041 agccgaccgc gagagaaacg accgtggtcc gcgcgatcac cggacggcca ccgaccccga
     2101 tgctgtgcgg ttggatgccg tccaggtcga tgccggcttc gcgcgcgacc caggcagcga
     2161 aacgattgtg gagcgatccg gtgtccagca gacaggccag cgccgcatcg tccaggccgc
     2221 cgactttgac atccaccagc ggccggcgaa cgtcctcggc cgggccggga agagatcgaa
     2281 agccgatctt cacgacgcga agcctccgac atcggcggcg ggatccagcg gaacgcgaaa
     2341 gagcgactcg gccttgatgc ccgccgcccg gagccccgcg aggacggcct gcggcgaatc
     2401 ggcggcgatc acgacctgac ccgcgcgggt cgcgacccac cggccgaggt acggccggag
     2461 cgcatcggcg tcagagtgac ggtacgcggg agcggactcg gcggcgcggg gcggctttga
     2521 ttcgccggcg cggcccgatg cgaagcccag tcgccgcaag atcgaccgcg cctgattggt
     2581 ggtgaagtcg ttccgatcga gtccggtgat cgcgccgatg atctgcttgg gcgggaagcg
     2641 ccggccctcg atgacggtgt agtggtcgcg caccgggtcg ggatacaggt cgcggcagat
     2701 ggcctcgatc cgctcgcgcg acagggtgaa cgagtggccc gcgacgacga ccgtctcttt
     2761 tttcatcgta ctttttactc tatcatcgcc gggccgagtt tccgccgcct cgcggggcga
     2821 atatcctgcg cgccgacatg ttggtcatcg tcgctcccgc aacgttcaaa gggtcgctgg
     2881 ccgcgaccga cgccgccgag gcgatcgccg cgggcgtgcg cgccgccggc ggcacgcctg
     2941 tgctgctgcc ggtcgccgac ggcggcgaag gcacgctcga cgcgctgatc gccggcgcgg
     3001 gcggcacgat catgggtgtg atcgcgcgcg gcccgctggg gctgccggtg cgcgggcatc
     3061 tggggcggct caccgacggc accggcgtgg tcgagatggc acaagccagc ggcctgatgc
     3121 tggtcgccgc gcacgaacgg cagccgatgc ggg
//
LOCUS       DATLFO010000148         6398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_84382, whole genome shotgun sequence.
ACCESSION   DATLFO010000148 DATLFO010000000
VERSION     DATLFO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84382"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VM841_05670"
     CDS             complement(<1..842)
                     /locus_tag="VM841_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89706.1"
                     /translation="MIRAALVAMLALAAMLPGHARAETPNAAVNGFARASAAFIPFNV
                     NPNFDFWPVYSLSETDGSFSHGLAGGFWPGFLVDATFFQFGYQPFERALFGVAESAYP
                     ITPFEDTASSTDFAEMCAAGEKSTVPLPDNQGDLGAPEPMLTGCQSLYTQYKNRIPYS
                     VSDARTVSGELFSDSSATGVELRLGTIVAGSAYSSSFTDASQGGKTVSGATVLLSDIK
                     ITDALRIEQLRSVVRTVADGTAKGAKIERTLEILNARFQQQPVRIGEDGVTLPDGTPS
                     SPMRT"
     gene            complement(868..1578)
                     /locus_tag="VM841_05675"
     CDS             complement(868..1578)
                     /locus_tag="VM841_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89707.1"
                     /translation="MLAVRDIDVFYGRVQALRSVTLEIGPGEMVALFGANGAGKTTTL
                     RAITGMLPLTTGEVVFDGTKLNAMSPEAINALGIAHIPEGRGIFPHMTVWQNLKMGGY
                     AKRLKAGPLAEQVDRVVGLFPRLKERLGQQAGTMSGGEQQMLAIGRALVGSPRLLLLD
                     EPSHGLAPKIVQEVFALLANLRTEGMGLLVVEQYAAVALSVAERGYVLDRGAVAFAGD
                     AESIASDPNALVGAYLGA"
     gene            complement(1562..3490)
                     /locus_tag="VM841_05680"
     CDS             complement(1562..3490)
                     /locus_tag="VM841_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVL89708.1"
                     /translation="MKGGGNLAYGAGLALAVALAGSVLGAFWLTIFSLAVCYVLAALS
                     LVVLTGWSGQLNLHVAALGLGWGAYSMYALTVFGLPVAIALPVAGIVTVPFAVAIAAV
                     AVRFRGLELAVATLAIGLIFERLAFRNIGKVLASRSENITTFGSSFVPVGRPDAGVVS
                     FESDRGFFYFALLMALVVFLLVRNLARGRTGRTFRAVREREVMAESLGIPVLRWRVGA
                     FALSIMIAATAGGLLASLKTGITPDSFNIDLSFRLLAATVIGGIVSPAGAVVGGAVAA
                     LLPEIVQFGPLRIFSGERLFILFGVGMILALWGLPEGLAGLGGRIRARRAHRSLPAPD
                     PLPDALPAAAPSPNGHPAVRRIYPNALARHMRPTLLRAHGIKVRFGGVVALDGVDLSV
                     PEGEIAALIGPNGAGKSTLFNCISGLLEPNEGRIYYRGRDVTGDGAHRRALEGIGRTF
                     QTVEAFREMTVIENLMVAGQSTVPASVGAEAFLLPSSRRAERTLRDRAHEICETIGLS
                     DVTDRLAAELSLGRLRELELGMALVRRPRLLLLDEPSAGLDPRETAALGEIVRRARDA
                     QDLSVLLVEHDMDLVQSLAEHVFVLDFGEIIAQGSPEQVRRDPVVLERYLGSADLTLA
                     AAAGDGTERKAVSRARRS"
     gene            complement(3487..4368)
                     /locus_tag="VM841_05685"
     CDS             complement(3487..4368)
                     /locus_tag="VM841_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL89709.1"
                     /translation="MVFFLQYVVNGLAAGFVIGLLALSLVMIYRSTRVLSFAQGVIAS
                     LGTYVYYQMQTVWEWHVLIAVPAALVVAAIVGALADLLAMRPLRKADALTRTVATLGI
                     VLVLQVVMRAGWGGQESFVRPIVSGGISAGQFSVGAQELLTAFLAIAAAGLFGLWART
                     SYRGLGLQAIAEDASAARLLGVDPTRASMLAWSIGAVLAALAGILVTPTLVLNPLQMT
                     LIMVTAFGAALLGGFTSLPMALGGGVLIGVVQSVATGYINVSGVSEAFGFIAVFAVLV
                     LARGKRGAVALPGRSVA"
     gene            complement(4378..5916)
                     /locus_tag="VM841_05690"
     CDS             complement(4378..5916)
                     /locus_tag="VM841_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89710.1"
                     /translation="MSALSEVRNATERERGRRKATAFRGGRPSVPTVRAGRARRLAVF
                     AAVVALLLTACGPRFDSDDVPTIAIGNDRPASEDGPQDQPGVPTTGPTSGGQVGALPT
                     AGTGGNGNGGIGGGKGAVVRGGVVKVGGLFPLTGGLSALGTPAFQGAQAYFNYLNSKG
                     GIGGKKIQFVVCDDQADDTRSTTCAKKLVEQDGVFMMGPSFTPFSLTVINQLRSAGVP
                     WVGYDGINVEGFSAENVVTIGSPIETMEHGLLPYWWDKVKRETGSPPQKLGVVVLDSG
                     PAQTYLREAEDVMCPRLGCQIVRTAEVGYTTTQYGTICRSMQSQNVDAVWIITDPASA
                     IKLLVQCQAAGYKPPKGFLGQHGIYLDLTLRQAGKFADGILSNSALVPDTVDNPATRE
                     MKKIIRTYYPNAEMGYFTTLAYASARMVADVIEGALDTGKELTRSEALGAAAKITSYD
                     CHGLCKGVNLAPPAARTGGNHNIWIVRADFSQDSKGRWVYEAGPIEAYSVRTWPCPGK
                     PRPC"
     gene            6193..>6398
                     /locus_tag="VM841_05695"
     CDS             6193..>6398
                     /locus_tag="VM841_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89711.1"
                     /translation="MRRRLETAAEAIGVRALFFDAFTRIGDVLEACAVVVDLDVPGWE
                     GAVEIVRERWPKAMAAAFISIPDK"
BASE COUNT         1108 a         2164 c         2199 g          927 t
ORIGIN      
        1 gttcgcatcg gcgaggacgg cgtcccgtcg ggcagcgtga cgccgtcctc gccgatgcga
       61 acgggctgct gctggaatcg cgcgttgagg atctcgagcg tgcgctcgat cttggcgccc
      121 ttggccgttc cgtcggccac ggtccgcacc accgaccgga gctgctcgat gcgcagcgcg
      181 tcggtgatct tgatgtcgct gaggaggacc gtggcgccgg agacggtctt gccgccctgc
      241 gatgcgtcgg tgaacgagct cgagtacgcg gatccggcga cgatcgtgcc cagccgaagc
      301 tcgacgccgg tcgcggagga gtcgctgaac agctcgccgc tcacggttcg cgcgtcggaa
      361 acgctgtagg gaatgcggtt cttgtactgg gtgtagagcg attggcagcc ggtcagcatc
      421 ggctcgggcg cgcccaggtc gccctgatta tccggaagcg gcaccgtgga cttctcgccg
      481 gccgcgcaca tctcggcgaa gtccgtcgag gaggcggtgt cttcgaacgg agtgatcggg
      541 tacgccgact cggccacgcc gaacagggcg cgctcgaacg gctgatagcc gaactggaag
      601 aaggttgcat ccacgaggaa accgggccag aagccgccgg ccaggccgtg gctgaacgag
      661 ccgtcggtct cgctgaggct gtagaccggc cagaagtcga agttcgggtt gacgttgaac
      721 gggatgaaag cggccgaggc gcgagcgaat ccgttgacgg cggcgttcgg cgtctcggct
      781 cgcgcatggc cggggagcat ggccgcgagc gccagcatcg cgacgagcgc cgcacggatc
      841 attctcgctg ccctcttcat ctgttcgtca cgctcccagg tatgcgccca cgagcgcatt
      901 cggatcggag gcgatcgatt cggcatcccc ggcgaacgcc accgcaccac ggtcgagcac
      961 gtagccacgc tcggccaccg acagggcgac cgctgcgtac tgctcgacca ccagcaagcc
     1021 catgccttcc gttcggaggt tcgccaaaag cgcgaagacc tcctgcacga tcttgggggc
     1081 gagaccgtgg gagggctcgt ccaacagcag caaacggggc gaaccgacca aggcccgtcc
     1141 gatcgcgagc atctgctgct cgccgcccga catcgttccg gcttgctgcc cgagccgttc
     1201 cttcaaccgc gggaaaaggc ccacgacccg gtcgacctgc tcggccagcg gcccggcctt
     1261 gaggcgcttg gcgtagccgc ccatcttgag gttctgccag accgtcatgt gcggaaagat
     1321 cccccggccc tcggggatgt gcgcgatgcc caaggcgttg atcgcctcgg ggctcatggc
     1381 gttgagcttc gtgccgtcga agacaacctc gccggtcgtc aacgggagca tgccggtgat
     1441 cgcccgcagg gtggtggtct tgcccgcgcc gttggcgccg aacaacgcga ccatctcgcc
     1501 cggcccgatc tcgagcgtga ccgagcgcag cgcctgcacg cgtccgtaga aaacgtcgat
     1561 gtcacgaacg gcgagcacgg ctcaccgcct tccgttcggt cccgtcaccg gccgccgcgg
     1621 caagcgtgag gtcggcgctg ccaaggtacc gttcgagcac gaccggatcg cggcgcacct
     1681 gctcgggcga tccttgcgcg atgatctcgc cgaagtcgag cacgaagacg tgttccgcga
     1741 ggctttgcac cagatccatg tcgtgttcga cgagcagcac cgacagatcc tgcgcatcgc
     1801 gcgcccgccg gacgatctcg ccgagcgccg cggtctcgcg cgggtccagg ccggcggagg
     1861 gctcgtcgag cagcagcagg cgcgggcggc gcacgagcgc catcccgagc tcgagctcgc
     1921 gcagccgtcc gagcgaaagt tcggccgcga gacggtccgt gacgtcgctg agaccgatgg
     1981 tctcgcaaat ctcgtgggcg cgatcgcgga gcgtgcgctc ggcacggcgc gacgacggca
     2041 aaagaaacgc ctcggcgccg acgctcgcgg gcaccgtgct ctggcccgcg accatcaggt
     2101 tctcgatcac ggtcatctcg cggaatgcct cgacggtttg gaatgtgcgg ccgatgccct
     2161 ccaacgcccg gcggtgggcg ccgtcaccgg tcacgtcgcg gccgcgatag tagatgcggc
     2221 cctcgttcgg ctccagcaaa ccggaaatgc agttgaacaa tgtggacttc ccggcgccgt
     2281 tgggcccgat cagcgcggcg atctctccct ccggcaccga caggtcgaca ccgtcgagcg
     2341 cgacgacgcc gccgaagcga accttgatcc cgtgcgcgcg cagcagcgtc ggacgcatgt
     2401 gccgtgcgag cgcgttcggg tagatgcggc gcacggcagg atggccgttg ggtgacggcg
     2461 ccgcggccgg cagggcgtcg ggaagcggat cgggggcggg cagcgagcga tgcgcgcgcc
     2521 gcgcacgaat gcgcccgccg aggccggcaa gcccttcggg cagcccccag agcgcaagga
     2581 tcatgccgac gccgaacagg atgaacaggc gctcgcccga gaagatgcgc agcggaccga
     2641 actgcacgat ctcgggaagc agggcagcga cggcgcctcc gaccaccgcg ccggccggcg
     2701 agacgatgcc gccgatcacc gtcgcggcga gcagccggaa cgacaggtcg atgttgaacg
     2761 agtccggcgt gatgccggtc ttgagcgagg cgagaagccc gcccgcggtc gcggcgatca
     2821 tgatcgaaag cgcgaacgcg ccgacgcgcc agcgcagcac agggatcccg agcgactcgg
     2881 ccatgacctc gcgctctcgc accgcgcgga aggtccggcc cgtccgcccg cgagcgagat
     2941 tgcggaccag aaggaagacg acaagggcca tcagaagcgc gaagtagaag aagccgcggt
     3001 cgctctcgaa cgaaacgacc ccggcatcgg gccgtccgac cggaacgaac gacgagccga
     3061 aggtggtgat gttctcgctg cgcgaggcga ggaccttgcc gatgttgcga aacgcgagcc
     3121 gctcgaagat caagccgatc gcgagcgtgg cgacggcgag ctcgaggccg cggaaccgaa
     3181 cggcgactgc ggcgatcgcg acggcaaacg gaacggtcac aatgccggcg acgggaagcg
     3241 cgatcgcgac gggaaggccg aagacggtca gcgcgtacat cgagtacgcg ccccacccga
     3301 gcccgagcgc ggcgacgtgc aggttgagct gcccgctcca gccggtgagc accacgagcg
     3361 aaagcgcggc cagcacgtag cagacggcga gcgagaagat cgtcagccag aacgcgccga
     3421 gcacggagcc cgcgagcgcg accgcgaggg cgagcccggc cccgtacgcg agattgccgc
     3481 cccccttcat gccacgctcc ttccgggcag cgcgaccgcg cctcgcttgc cacgcgcgag
     3541 caccagcacg gcaaacaccg cgatgaaccc gaaggcctcc gacacgccgg agacgttgat
     3601 gtagccggtg gccacggact gcacgactcc gatcagcaca ccgccgccga gcgccatcgg
     3661 caggctcgtg aacccgccga gcagcgccgc accgaaggcg gtcaccatga tgagcgtcat
     3721 ctgcaacggg ttgagaacca gggtcggtgt caccagaatg ccggcgagcg cggcgagcac
     3781 cgcgccgatc gaccaggcga gcatcgacgc ccgggtcggg tccacgccca gcagacgcgc
     3841 ggccgacgcg tcctcggcga tcgcctgcag gccgagtccg cgataggacg tgcgcgccca
     3901 cagaccgaac aggcctgccg cggcgatggc gaggaaggcg gtgagcagct cttgcgcgcc
     3961 cacgctgaac tggccggcgc tgatgccgcc gctgacgatc ggacgcacga aggactcctg
     4021 cccgccccag cccgcgcgca tcacgacctg aaggaccaga acgatcccga gcgtcgcgac
     4081 cgttcgcgtg agcgcgtcgg ccttgcgcag cgggcgcatc gccaggagat cggcgagcgc
     4141 gccgacgatc gcggccacga cgagcgcggc cggaaccgcg atcagcacgt gccattccca
     4201 cacggtctgc atttggtagt agacgtaggt gccgagcgag gcaatgacgc cctgggcgaa
     4261 agacagcacc cgggtcgatc gatagatcat caccagcgac agcgcgagca gcccgatgac
     4321 gaacccggct gcgaggccgt tcaccacgta ctggagaaag aacaccatcc cggccgccta
     4381 gcagggtctc ggcttgccgg ggcacggcca tgtccggacc gagtacgcct cgatcggccc
     4441 ggcctcgtag acccagcggc ccttcgaatc ctgcgagaag tccgcgcgaa cgatccagat
     4501 gttgtgattg ccgccggttc gcgcagccgg tggcgcgagg ttcacgccct tgcacagacc
     4561 atggcagtcg taggaggtga tcttcgccgc ggcaccgagc gcctccgagc gcgttagctc
     4621 cttccccgtg tcgagcgccc cttcgatcac atccgcaacc atacgagcgc tcgcatacgc
     4681 cagcgtcgtg aagtagccca tctccgcgtt cgggtagtag gtgcggatga tcttcttcat
     4741 ctcgcgcgtc gcagggttgt cgaccgtgtc gggcacgagc gcggagttgg acaggatccc
     4801 gtcggcgaac tttccggcct gacgaagcgt gaggtccaga tagatgccgt gctgcccgag
     4861 gaagcccttc ggcggcttgt agcccgcggc ctggcactgc acgagaagct tgatggcgct
     4921 ggcggggtcg gtgatgatcc aaaccgcgtc cacgttctgc gactgcatcg agcggcagat
     4981 cgttccgtac tgcgtggtgg tgtagcccac ctcggcggtg cgaacgatct ggcatccgag
     5041 ccgcgggcac atcacgtcct cggcctcgcg cagatacgtc tgggccggac cggagtccag
     5101 cacaacgacg ccgagcttct gtgggggcga gcccgtctcc cgtttgacct tgtcccacca
     5161 gtacggcagc aggccgtgct ccatcgtctc gatcggcgag ccgatggtca cgacgttctc
     5221 ggcggagaac ccctcgacgt tgatgccgtc gtagccgacc cacgggacgc cggccgagcg
     5281 aagctggttg atgaccgtca gcgagaacgg tgtgaaggac ggtcccatca tgaagacgcc
     5341 gtcctgctcg acgagcttct tcgcgcacgt ggttgagcgg gtgtcgtcgg cctggtcgtc
     5401 gcacacgacg aactggatct tcttcccgcc gatgccgccc ttggagttca gatagttgaa
     5461 gtaggcctgc gcgccctgga acgccggggt cccgagcgcc gacaggccac cggtaagcgg
     5521 gaacaggccg ccgaccttga cgacgccgcc gcgaacgact gcgcccttgc cgccgccgat
     5581 cccgccgttc ccgttgccgc cggtgccggc ggtcggcagt gctcccacct gcccgcccga
     5641 tgtcggcccg gtggtgggca ccccaggctg gtcttgggga ccgtcctccg acgcgggacg
     5701 gtcgttgccg atggcgatgg tggggacatc gtcgctgtcg aatcgcggac cgcaggcggt
     5761 gaggagcaag gccaccacgg ccgcgaagac ggccaggcgg cgtgcgcgtc cggcgcgaac
     5821 ggtgggaacc gatggtcggc ccccgcggaa ggccgtcgcc tttcgacggc cgcgctcacg
     5881 ctctgttgcg ttgcgaactt cggatagagc gctcaccccg cccctctccg ccggcttgcc
     5941 gcactcatgc gcgtatgtgc atgtgtgcaa gggctaatgt aggtttcgag cgagggggag
     6001 tcaatggtga aatcacccgg cagagcgacc tctgcggggg atctcgactt tcctcccggt
     6061 tgcaccgtag acaccatcgt tccaaagttt acattgaaac caccgtaata accagtgaaa
     6121 gaccgaaagt cttctttggg ggcatctcag ttgatcccag gagccttgat cgtcgtatcg
     6181 cggaatccga tcgtgcgccg ccggctggag acggccgccg aggcgatcgg cgtccgcgcc
     6241 ctgttcttcg acgccttcac ccggatcggc gacgtgctgg aggcgtgcgc cgtcgtggtg
     6301 gatctcgacg tccccggctg ggagggcgcg gtcgagatcg tccgcgagcg gtggccgaag
     6361 gccatggcgg ccgccttcat ctcgatcccg gacaagga
//
LOCUS       DATLFO010000149         9304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_84400, whole genome shotgun sequence.
ACCESSION   DATLFO010000149 DATLFO010000000
VERSION     DATLFO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84400"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VM841_05700"
     CDS             complement(<1..1399)
                     /locus_tag="VM841_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVL89712.1"
                     /translation="MSGGRTAAQTIAGFAAALTTEDVPEPVQRSAILHLLDGFGCAIA
                     AHRAKAVWFAVDAALKDPEPEQATVIGEGRRVATTAAAMANGALVHALDFDDTHEEAL
                     VHATAAVLPAAFAVGEERHASGAQVLAAVIAGYETVIRLGAAVPHGFHRRGLHATSVC
                     GVFAAAVAASKLLGSHADQIANALGIAGSMSAGSLEFLNAGSATKQLHPGLSAMNGIV
                     AARLAFEGAEGPATVFEGEHGLYRTLAGVRVDPAALTRGLGETWETARIAIKPYPACQ
                     LSHAAFDALRQVEDLDPDAIERFVIEVPASSLPIVCEPREIKSAPRNAYDAKFSLPWS
                     AAALVIDGRLDLRTYQDVSRPDVKALAARIECVGVAYDGAPARAPAEVEITVRSGRVI
                     RRSVTPGGTLDEAALMTKLRDNCGPLDAEALAAAVLALPASPTLKELASALDLRGGQR
                     QHRRREGLRVERSAVV"
     gene            complement(1444..2646)
                     /locus_tag="VM841_05705"
     CDS             complement(1444..2646)
                     /locus_tag="VM841_05705"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006437454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVL89713.1"
                     /translation="MRPLEDVRVIALEQYGAGPFGSLHLADLGAEIIKIEDPSTGGDV
                     GRYVPPFQHEQDSLFFETFNRNKRSIVLDVTNPAGREVLEDLIRVSDAMYSNLRGDVP
                     ERLRITYEHLKSVNGKIVCVSLTGFGMTGPRKAEPGYDYVLQGLAGWMSLTGEPDGPP
                     AKSGLSLVDYIGGIVAALSLVSGIHAARRDGRGMDCDVSLYDAAIGMLTYPAVWHLNA
                     GFTPARHPRSAHPSLVPFQLFPTADGWIVVGCAKEKFWQRLAAALDKPELASDDRFKD
                     FAARNDNRDELIAILDEAFMTKTGAAWLSLLAEAGVPCGPVNDVEQAFADSHTEARGL
                     IVETEHPRFGIVRQPASPVRVGEPAASHRRAPRLGEDEPYVLGGLLGYGETRIASLRD
                     AGAFGAPR"
     gene            complement(2713..2934)
                     /locus_tag="VM841_05710"
     CDS             complement(2713..2934)
                     /locus_tag="VM841_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89714.1"
                     /translation="MAKKDQLETGDRVRVVALRQDPQGDPVPDDVVGLEGTIQWITPG
                     FAGEKAVFEIKLTDGRVVNLYAPEIEAIS"
     gene            2980..3492
                     /locus_tag="VM841_05715"
     CDS             2980..3492
                     /locus_tag="VM841_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HVL89715.1"
                     /translation="MTSREYHSDELVVRWNAERCIHAADCIASAPAVFDTSQRPWVRL
                     DKGSTDEIVAAIERCPTGALSYERVGAPEAPPPQTTASAVPGGPLLIRGPITIYDEDG
                     SVLAEGSRFAICRCGRSKRQPFCDNSHRLPGERRPAEPVSPLQICPRQPDEFVKRSAN
                     VYDASSDASS"
     gene            complement(3462..4154)
                     /locus_tag="VM841_05720"
     CDS             complement(3462..4154)
                     /locus_tag="VM841_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YrzE family protein"
                     /protein_id="HVL89716.1"
                     /translation="MARRQRTIDPVTRETIVLDGPDATSVPPADMAEYPPPETRVVRE
                     RVPEAMVIRGGFSPGAVLTGMLTAFGVMLVLSAIVGAIAANSSLDAAGLDRGEAIGLG
                     IGAAAAFVLAQFLAYFWGGYTAGRMARGKGVLHGLLVPVFAAVVAGITALIVAALGSS
                     MRIVMPFGDGRFSIDADTVYTFGAAVGIAALAAMVLGGMAGGMAGTRWHTKLERKAAR
                     ERYYDEASDEAS"
     gene            complement(4214..5224)
                     /locus_tag="VM841_05725"
     CDS             complement(4214..5224)
                     /locus_tag="VM841_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVL89717.1"
                     /translation="MTARDLARELRSVNRFDRHAYMRALEEVDFDTALQIVRRCLERG
                     VPRDAIVGDLLGPLQEEIGERWEEGRCSVADEHAASGITESVVTALSFDRPAAQCGAV
                     VMACVEHEWHSLPARMAGLLLADRGWQVTLLGASVPAEDIGRFTEGRSLAGLAVSCST
                     PATLLPARRMIEAAHAKGLRVLAGGRGFGTDGERARRLGADGWGANVEHAHRTLQAWQ
                     ERPAGGPLVEPPLDREAETIAESTHELAIGMAEELAAAGAEHAGSLTRLLEDGGRALQ
                     MIASAAIVDEPGIVGEHIRWLRGVSAARYGEGRYVDTLIRALRAALKELPGTGRILPV
                     LT"
     gene            complement(5221..6069)
                     /locus_tag="VM841_05730"
     CDS             complement(5221..6069)
                     /locus_tag="VM841_05730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL89718.1"
                     /translation="MTGRSHRSAGNSESAPGSDAAARADPWWTAYEATRSILHATTVP
                     DVVDAVSRFVRKCEGRVVPGDEAPPGALPLDVSLGGRSPLLAVAEGATARRHLARFLP
                     RVVEDARLAAGRIRHEERLATDATVDPLTGVLNRRGLARVVPRLSDGDAVVALDVDEL
                     DGVNQMHGTEGGDAVLTGFAQMIAEQARLDDRCGRTGGDEFVLTMRRTSVDGALALVR
                     RMLARWQQIAPHPVTFSAGIANVDEGPPARALVRADRALSRAKQSGGNAVEVILSVDD
                     ADLPDR"
     gene            6268..7407
                     /locus_tag="VM841_05735"
     CDS             6268..7407
                     /locus_tag="VM841_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVL89719.1"
                     /translation="MDSPRRTVVDALLALALLGAQLPSVVGWPGPSRATQALPLLLVG
                     LQTVPVVWRRSAPVLVLAVTCAAYVFHASLDHGYTSPFFAPAVIYAVAAYAEARVSIA
                     AGALGLVAIAGATGLEVADHRSGIADVAFISTVNVVAWVVGFRIRAARERERSLARRA
                     AELETGRAAAAREAVERERARIARELHDVVAHGVGVMVVQAAGARRVIGRDPAAATDA
                     LAAIEGTGRTALAELRRLLGILHDDEPASLEPLPGIDSIETLAERLRAAGLAVEVRHE
                     GERRDVPAAVGLSIYRIVQEALTNTLKHARASRATVTVAYRPGSVGVVVRDDGAGAAE
                     ANGSGRGLAGMRERVALFDGTLSAGPHPAGGFAVEAHLPVAEGGE"
     gene            7404..8075
                     /locus_tag="VM841_05740"
     CDS             7404..8075
                     /locus_tag="VM841_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89720.1"
                     /translation="MTIRVLIADDQSLVRAGFRMILDAEPDIEIAGEAPDGEQAVAAA
                     RSLRPDVVLMDVRMPVMDGIEATRRLAGPGRADPVKVVILTTFDLDEYVVEALRAGAS
                     GFLLKDAPPDDLVEAVRVVADGNALLAPSVTRKLLDKFAQRLPAPSADPAPRLAGLTE
                     RETEVLTLMARGLSNAEIAASLFVGEATVKTHVSRVLMKLGLRDRVHAVIFAFESGLI
                     RPASG"
     gene            8202..8930
                     /locus_tag="VM841_05745"
     CDS             8202..8930
                     /locus_tag="VM841_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL89721.1"
                     /translation="MEIKEMVAPIAARGLTKRYGAETVVEDLSFSTRWGAVTGFVGPN
                     GAGKSTTMRMIAGLVRPTGGEALVMGRPYAALDDPSRFVGASLEAGAFHPRRTGRNHL
                     RVLAAAAGLTDRRVGEVLDLVELGPAAKKNVGAYSLGMRQRLGLAAALIGDPSILMLD
                     EPANGLDPAGIRWLRSFLRGYVEAGNAALVSSHLLGEVAQIADDVVVIRGGRLVRHAT
                     VAELTAGGSLEDVYFELTEQEAAR"
     gene            8927..>9304
                     /locus_tag="VM841_05750"
     CDS             8927..>9304
                     /locus_tag="VM841_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89722.1"
                     /translation="MKTDIHSEIRKLISVRSSWALAAGAVAIAVATVLAAAAAGEMPV
                     GDPLHNLLVLRMPLFTGIVLLVLGIRSVTDEFRYGTMAATVLASPGRMRVIGAKAVVA
                     AIVPYRNSSVTLRMPSTRSTMPVN"
BASE COUNT         1299 a         3353 c         3259 g         1393 t
ORIGIN      
        1 cgacaactgc ggaccgctcg acgcggaggc cctcgcggcg gcggtgctgg cgctgcccgc
       61 ctcgcaggtc cagcgcggag gcgagctctt tgagcgtcgg cgaggcgggc agcgccagca
      121 ccgccgccgc gagggcctcc gcgtcgagcg gtccgcagtt gtcgcgcagc ttcgtcatca
      181 acgccgcctc atcgagcgtg ccgcccggcg tgacgctgcg gcggatcaca cgcccgctgc
      241 gcacggtgat ctcgacctcc gccggcgcgc gggccggtgc cccgtcgtag gcgacgccga
      301 cgcattcgat gcgcgcggcc agcgccttca cgtccggccg cgatacgtcc tgataggtgc
      361 gaaggtcgag ccgtccgtcg atcaccagcg cggcggcgct ccacggcagg ctgaacttgg
      421 cgtcgtaggc gttgcgcggt gcgctcttga tctcgcgcgg ctcgcacacg atcggcaggc
      481 tcgacgcggg aacctcgatg acgaaccgct cgatcgcgtc cgggtccagg tcctcgacct
      541 gccgcagcgc gtcgaaggct gcgtgcgaga gctggcacgc cgggtagggc ttgatggcga
      601 tgcgtgcggt ctcccaggtc tcgccgagcc cgcgggtgag cgcggcggga tcgacgcgta
      661 cgccggcgag cgtccggtac aggccgtgct cgccttcgaa gacggtggcc ggcccttcgg
      721 cgccttcgaa cgcgagccgc gcggcgacga tcccgttcat cgcgctcaac cccgggtgca
      781 gctgcttggt cgcagagccg gcgttcagaa actccagcga tcccgcggac atgctgcccg
      841 cgatccccag tgcgttcgcg atctggtcgg catgtgagcc cagcagcttg gaggccgcga
      901 cggcggccgc gaataccccg cacacgctgg tcgcgtgcag cccgcggcga tggaagccgt
      961 gcggcacggc cgcgcccagc cggatcacgg tctcatagcc ggcgatcacg gcggcgagca
     1021 cctgggcgcc gctcgcgtgg cgctcctcgc cgaccgcgaa cgccgccggt agcaccgccg
     1081 cggtggcgtg cacgagcgcc tcttcgtgcg tgtcgtcgaa gtcgagcgcg tgcacgagcg
     1141 cgccgttggc catcgccgcc gcggtcgtcg ccacgcgccg tccctcaccg atcaccgttg
     1201 cctgctcggg ctcggggtcc ttcagggccg cgtccaccgc gaaccagacg gccttcgcgc
     1261 ggtgcgcggc gatcgcgcag ccgaagccgt cgagcagatg cagtatcgcg gaacgctgca
     1321 ccggctctgg tacgtcctcc gtcgtcagcg cggccgcgaa gcccgcgatc gtctgcgccg
     1381 ccgttcgtcc gccgctcacg ggctcacgat actcccggcc ggaatcgccg ttccagtttc
     1441 ccgctatcgg ggcgcgccga acgcgccggc gtcgcgcagc gaggcgatgc gcgtctcgcc
     1501 gtagccgaga agcccgccga gcacgtacgg ctcgtcctcg ccgaggcgcg gcgcgcgacg
     1561 gtgcgaggcg gccggctcgc cgacgcggac cggcgaggcg ggttgccgca cgatgccgaa
     1621 tcgcggatgc tcggtctcga cgatgaggcc gcgcgcttcg gtgtgtgagt cggcgaaggc
     1681 ctgctcgacg tcgttgaccg gaccgcacgg aacgccggcc tcggcgagca gcgacagcca
     1741 cgcggctccg gtcttcgtca taaacgcctc gtcgaggatc gcgatcagct cgtcccggtt
     1801 gtcgttgcgt gcggcgaagt ctttgaagcg atcgtcggat gccagctcgg gcttgtcgag
     1861 cgcggccgcc aaacgctgcc aaaacttttc tttcgcgcag ccgaccacga tccagccgtc
     1921 cgcggtggga aacagctgga aagggaccag cgacggatgg gcgctgcgcg ggtggcgcgc
     1981 cggcgtgaag ccggcgttca agtgccacac cgccgggtag gtcagcatgc cgatcgccgc
     2041 gtcgtagagc gagacgtcgc agtccatgcc gcggccgtcg cgccgcgcgg cgtggatgcc
     2101 cgacaccagc gaaagcgccg cgacgatgcc gccgatgtag tccacgagcg atagcccgga
     2161 cttggcgggt gggccgtcgg gctcaccggt cagcgacatc catccggcca aaccctgcag
     2221 cacgtagtcg tagccgggct cggccttgcg cgggcccgtc atgccgaagc ccgtcaagga
     2281 cacgcatacg atcttgccgt tgaccgactt cagatgctcg taggtgatgc ggagccgttc
     2341 cggcacgtcg ccgcgcaggt tcgagtacat cgcgtcgctg acgcggatca gatcctcgag
     2401 cacctcgcgg cctgccgggt tggtcacgtc cagcacgatc gagcgtttgt tgcggttgaa
     2461 cgtctcgaag aacagcgagt cctgctcgtg ctggaacggc ggcacgtacc ggccgacgtc
     2521 gccgcccgtg gacggatctt cgatcttgat gatctcagcg ccgagatccg cgagatgcag
     2581 cgagccgaac gggcccgcgc cgtactgctc gagcgcgatc acacgcacgt cctcgagcgg
     2641 cctcatgccg ggcaagggta ccatccaggt ttcgctcaga gaaacgtcgg atcgctcagc
     2701 ggtatcgctt cgttacgaga tcgcttcgat ctccggcgcg tacagattca ccacgcgacc
     2761 gtcggtgagc ttgatctcga acactgcttt ctcgcccgcg aagccgggcg tgatccattg
     2821 gatcgtcccc tccagcccga ccacatcgtc gggaaccgga tccccttgcg gatcttgacg
     2881 caacgccacg acgcgcacgc gatcgccggt ctcgagctga tctttcttgg ccatggcata
     2941 ttctccctgc cgcacgggtt cctgggtaca ctttcaccga tgacctcccg cgaataccac
     3001 tccgatgagc tggtcgtccg ctggaacgcc gaacggtgca tccacgcggc cgactgcatc
     3061 gcctccgccc cggccgtgtt cgacacgagc cagcggccct gggtccgcct cgacaaggga
     3121 tcgaccgacg agatcgtcgc ggcgatcgag cgctgcccga ccggcgcgct cagctacgag
     3181 cgcgtcggcg cgccggaggc cccgccgccg cagacgacgg cgagcgcggt gccgggaggc
     3241 ccgctgctga ttcgcgggcc gatcacgatc tacgacgagg acggcagcgt gctcgccgag
     3301 ggctcgcggt tcgcgatctg tcgctgcggc cgcagcaagc gccagccgtt ctgcgacaac
     3361 tcccaccggc tccccggcga gcgccggccg gccgagccgg tctcgccgct gcagatctgc
     3421 ccgcggcagc ccgacgagtt cgtcaagcgt tccgcgaacg tctacgacgc ctcgtccgac
     3481 gcctcgtcgt agtagcgctc gcgcgcggct tttcgctcca gcttcgtgtg ccagcgcgtg
     3541 ccggccatgc cgccggccat gccgcccagc accatcgcgg cgagcgcggc gatgccgacc
     3601 gccgcgccga aggtgtagac ggtgtcggca tcgatcgaga agcgcccgtc gccgaagggc
     3661 atcacgatgc gcatgctcga tccgagcgcc gccacgatca gcgccgtgat ccctgcgacc
     3721 acggccgcga agaccggaac gagcagcccg tgcagcacgc ctttgccgcg cgccatccgg
     3781 ccggcggtgt agccgcccca gaagtacgcg agaaactgcg cgagcacgaa agcggcggcc
     3841 gcgccgatgc ccagaccgat cgcctcgccg cggtccaggc cggcggcatc caggctgctg
     3901 ttcgccgcga tcgcaccgac gatcgccgac agcaccagca tcacaccgaa tgcggtgagc
     3961 atgccggtca ggacggcgcc gggggagaac ccaccgcgga tcaccatcgc ctcgggcacg
     4021 cgctcgcgca ccacccgggt ctcgggcggc ggatactcgg ccatatcggc ggggggaacc
     4081 gacgtcgcgt ccggtccgtc cagcacgatc gtttcgcggg tgaccgggtc gatggtgcgc
     4141 tgacggcgag ccatgggaga acctcctgtc ctttgcctcg ctagttcccc gggcgacccg
     4201 gtctgaaacg ggcctaggtc aggacgggca ggatccggcc ggttccgggc agctccttca
     4261 gcgccgcgcg aagcgcccgg atcaacgtgt ccacgtatcg gccctcgccg tatcgcgccg
     4321 ccgagacgcc tcggagccag cggatgtgct cgcccacgat ccccggctcg tccacgatcg
     4381 cggcggaggc gatcatctgc agcgcgcggc cgccgtcctc gagcagccgc gtcagactgc
     4441 ccgcgtgttc ggcgcctgcg gcggcgagct cctcggccat gccgatggcg agctcgtggg
     4501 tggactccgc gatcgtctct gcctcccgat ccaatggcgg ctcgacgagc gggccgcccg
     4561 ccggtctctc ctgccacgcc tgaagcgtcc ggtgcgcgtg ctcgacgttt gctccccagc
     4621 cgtcggcgcc gagccggcgc gcacgctcgc cgtccgtccc gaatcctcgc ccgcccgcca
     4681 ggacacgcaa tcccttcgcg tgcgccgcct cgatcatgcg gcgtgccgga agcagtgtcg
     4741 ccggtgtgga gcaggacacg gcgagccccg caaggcttcg gccttcggta aagcggccga
     4801 tgtcctcggc cggaaccgac gcgcccagaa gcgtcacctg ccagccccgg tccgcgagca
     4861 gcaatcccgc catgcgtgcg ggaagcgaat gccattcgtg ctcgacgcag gccatgacga
     4921 ccgcgccgca ctgcgcggca ggacggtcga aggaaagcgc ggtcaccacc gactccgtga
     4981 tgccgctggc ggcgtgctcg tcggcgaccg aacaccggcc ctcctcccac cgctcgccga
     5041 tttcctcctg gagggggccg agcaaatcgc ccacgatggc gtcgcgcgga acgccgcgct
     5101 cgagacagcg ccgcacgatc tgcagcgcgg tatcgaagtc cacctcttcg agcgcccgca
     5161 tataggcgtg gcggtcgaat cggttgaccg accgcagctc ccgggccaga tcgcgcgccg
     5221 tcatcggtcc gggagatcgg cgtcgtccac gctgaggatg acctccaccg cgttgcctcc
     5281 ggattgcttc gcccgcgaca gagcgcggtc cgcgcgtacg agggctcgtg cgggcggacc
     5341 ttcgtcgacg ttggcgatcc ccgccgagaa cgtgacggga tgcggtgcga tctgttgcca
     5401 ccgagcgagc atgcggcgaa ccagggcgag cgccccgtcg accgacgtgc gccgcatcgt
     5461 gagcacgaac tcgtccccgc cggttcggcc gcaacgatcg tcgagccgcg cctgctcggc
     5521 gatcatttgc gcgaaccccg tcaacaccgc gtcgcctcct tccgtgccgt gcatctggtt
     5581 gacgccgtcg agctcgtcca cgtccagcgc aacgaccgcg tcgccgtcgc tcaggcgcgg
     5641 cacgacgcgc gcgagcccgc ggcggttgag cactcccgtg agcggatcga ccgtcgcgtc
     5701 ggtggcgagg cgctcctcgt gacggatgcg tccggccgcg agccgcgcgt cctcgaccac
     5761 gcgcggcagg aaccgcgcga ggtgccgccg ggccgtcgcg ccttccgcga cggcgagcag
     5821 cgggctgcgg ccgcccaagg atacgtccag cggcagggcg cccggcgggg cttcgtcgcc
     5881 gggcaccacc cggccctcgc acttgcgcac gaatcgagag acggcgtcca cgacgtcggg
     5941 cacggtcgtc gcatgaagga tcgagcgcgt ggcctcgtac gcggtccacc aggggtcggc
     6001 gcgtgccgcc gcgtccgagc ccggagcgga ttcgctgttg cccgcggatc ggtggctgcg
     6061 tcccgtcatt cccgaccgat cctgccatcg cagcgccgcg tccgaccatg gccctctcga
     6121 aggatattgc cccgctgcgc acccgcttcg accggaatga gagatcccaa agatggccgt
     6181 gtcgcggacg cctcccccgg gcgggggagc ggcggcatcg gccgcgagga tgacgacccc
     6241 ggcccccgtc ccggctaatg tcggcgcatg gactcgcctc gccgcaccgt tgtggacgcg
     6301 ctgctcgccc tcgctctgct gggcgcccag ctgccgtccg tcgtcggatg gcccggtccg
     6361 tcgcgtgcga ctcaggccct gccgcttctg ctcgtggggc tccagaccgt gccggtcgtc
     6421 tggcgccgga gtgcccccgt gctcgtgctc gccgtcacgt gcgcggccta cgtatttcat
     6481 gcctcgctcg accacggcta cacgtcgccg ttcttcgcgc cggcggtgat ctacgcggtc
     6541 gcggcctatg ccgaggcgcg cgtgtcgatc gcggcgggcg cgctcggcct ggtggccatt
     6601 gcgggcgcaa ccgggctcga ggtcgccgac caccgctccg ggatcgccga cgtcgccttc
     6661 atctcgaccg tcaacgtcgt cgcatgggtg gtcgggtttc gcatccgtgc cgcgcgcgag
     6721 cgggaacgaa gcttggcccg cagggccgcc gagctggaga ccggccgggc ggccgccgcg
     6781 cgcgaggccg tcgaacggga gcgcgcccgc atcgcgcgcg agctgcacga cgtcgtcgcg
     6841 cacggggtcg gggtgatggt ggtgcaggcc gcgggcgcgc gccgggtcat cggccgggat
     6901 ccggccgccg ccaccgatgc gctcgccgcg atcgagggca ccggccgcac ggcgctagcc
     6961 gagcttcgcc ggctgctcgg catccttcac gacgacgagc ccgcctcgct ggagccgctg
     7021 cccgggatcg attcgatcga gacgctcgcg gagcggcttc gagcagccgg cctcgccgtc
     7081 gaagtccgtc acgagggcga gcggcgcgac gtgccggcag cggtcggcct gtccatctac
     7141 cggatcgtgc aggaggcgct gaccaacacg ctcaagcatg cgcgggcgtc gcgggcgacg
     7201 gtcacggtcg cctaccggcc gggatcggtg ggcgtggtcg tgcgcgacga cggcgccggc
     7261 gcggcggagg cgaacggatc gggacgcggc ctggcgggaa tgcgggaacg ggtcgcgctg
     7321 ttcgacggga cgctttcggc cggaccgcac cccgccggcg gcttcgccgt cgaggcgcac
     7381 ctgccggttg cggagggcgg cgaatgacga ttcgcgtgct tatcgccgac gaccagtcgc
     7441 tggtgcgcgc gggattccgc atgatcctcg acgccgagcc cgacatcgag atcgccggag
     7501 aggcgcccga cggcgaacag gcggtcgcgg cggcgcgttc gttgcgcccc gacgtcgtgc
     7561 tgatggatgt gcgcatgccg gtgatggacg gcatcgaggc cacacggcgc ctcgccgggc
     7621 cgggccgggc cgatccggtc aaggtcgtga tcctgaccac gttcgatctt gacgagtacg
     7681 tcgtcgaggc gctgcgcgcc ggcgcgagcg gctttctgct caaggacgcc ccgccggacg
     7741 atctcgtcga agccgttcgc gtcgtcgcgg acggcaacgc gctgctggcg ccgtcggtca
     7801 cgcgaaagct gctggacaag ttcgcgcagc gcctgccggc gccgtcggcg gatccggcgc
     7861 ccaggctcgc gggtcttacc gagcgagaga ccgaggtcct gaccctgatg gcgcgcggcc
     7921 tgtccaacgc cgagatcgcc gcgtcgctgt tcgtcggcga ggcgaccgtc aagacgcacg
     7981 tctcgcgcgt tctgatgaag ctcggccttc gggatcgagt tcacgcggtc atcttcgcgt
     8041 tcgaatccgg tctgatccgt cccgcctccg gctaggtccg tcgcgcggcg gagccgcggc
     8101 ctcccggaat catccgctcg cacgagccga agatcggccg tgcgccggat tccattcgtc
     8161 tctcccggtc atagcgtgac ggctgtcgaa gggagatcgt gatggaaatc aaagagatgg
     8221 tggcgccgat cgccgcgcgg ggcctgacga agcgctacgg tgccgagacg gtggtcgagg
     8281 atctgtcgtt ctcgacgcgc tggggcgcgg tgacgggatt cgtcggaccc aacggggcgg
     8341 gcaagtccac cacgatgcgg atgatcgccg gattggtccg gccgaccggt ggcgaggcgc
     8401 tcgtgatggg ccggccctac gcggcgctcg atgatccgtc ccggttcgtc ggcgcctcgc
     8461 tcgaggcggg cgccttccac ccccgccgca ccggacgcaa ccaccttcgg gtgctcgccg
     8521 cggccgcggg tttgaccgac cgtcgcgtgg gcgaggtgct cgatctggtc gagctcgggc
     8581 cggcggcgaa gaagaacgtc ggcgcgtact cgctggggat gcggcagcgg ctcggccttg
     8641 ctgccgccct gatcggggac ccgtcgattc tgatgctcga cgagccggcc aacggcttgg
     8701 acccggcggg cattcgatgg ctgcgctcgt tcctccgcgg atacgtcgaa gccggcaatg
     8761 cagctctcgt gtccagccac ctgctcggcg aggtcgccca gatcgccgac gacgtcgtgg
     8821 tgatcagggg cggccgactg gtgcggcacg cgaccgtggc cgagctcacg gccggcggct
     8881 cgctcgagga cgtgtacttc gagctcaccg aacaggaggc agcgcgatga agaccgacat
     8941 tcattccgag atccggaagc tgatcagcgt ccggtcgtcg tgggcgctcg cggccggagc
     9001 ggtcgcgatc gcggtcgcaa cggtcctcgc cgcagcggcg gcgggcgaga tgcccgtcgg
     9061 cgatccgctt cacaacctgt tggttctgcg catgcccctg ttcaccggca tcgtgcttct
     9121 ggtgctgggc atccggagcg tgaccgacga gttccgatac gggacgatgg ccgcgaccgt
     9181 gctcgcgtct ccgggacgaa tgcgtgtcat cggcgccaag gccgtcgtcg cggccatcgt
     9241 cccgtatcgg aactcgtcgg tcacgctccg gatgcccagc accagaagca cgatgccggt
     9301 gaac
//
LOCUS       DATLFO010000150         3012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_55330, whole genome shotgun sequence.
ACCESSION   DATLFO010000150 DATLFO010000000
VERSION     DATLFO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55330"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..573
                     /locus_tag="VM841_05755"
     CDS             43..573
                     /locus_tag="VM841_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVL89723.1"
                     /translation="MIRIASEDDSALSRRQALRLGFFGIAALAATELVAAFAPFMRVT
                     RFEGLGAKVAVPFTKAEILERFAATNDDPILFAQDKFFLLRPPEGIIAAYRKCTHLGC
                     AVPFAKHEDRFHCPCHQSIYDKRTALLISGPAPRGLDLFHMSEADGKLVVDTNPFELM
                     VRDDNKWDPAHVEVSG"
     gene            complement(609..755)
                     /locus_tag="VM841_05760"
     CDS             complement(609..755)
                     /locus_tag="VM841_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89724.1"
                     /translation="MSEARRRSDLVFVIGPAAVGKMTVGMERTAHRVEHRRAAYALLV
                     LGGR"
     gene            910..2667
                     /locus_tag="VM841_05765"
     CDS             910..2667
                     /locus_tag="VM841_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89725.1"
                     /translation="MKHRAGWLLTAVLVITGLAAGIGARPADASSAAVSGALASWLAT
                     APADAGFKTIVTFHDRSGMSRLDALAANQDLSVLPMSFAVLSAAQINTVRGWPEVVSL
                     WHDQENELILDESVALIGADKVHAGTKLKRGYTGLGVSVAVIDTGVDASHPDLTGRVA
                     MFQVAGDAFDRDGVAVLAAPTADTYGHGTHVSSTINGSGAASAGRYQGVAPGSQVYSF
                     KTDVGAFLLSSWALASFDWVLANPQAGIRVSSNSWGTMGPDDYDPANPINVTTKTMYD
                     RGITVIFAAGNSGGPNTLNQYAKSPWVVSVAAGDKAGKLASFSSRGRIDDNWDRRTAQ
                     GTNTGIYRPTITAPGVSIEAAKSAQATLMATGTDPANPFYTTASGTSMAAPHVAGVAA
                     LMLQARPGLKPQHIIDILEGTATDMRSYELFEAGIGYLDAHAAVFAAEKGRVIFPPAV
                     SGKTPTFTLASTTPFEGSVDTPNTWALARCKDDLGVLDHHLFEVAAGVGAIYAEIEWP
                     SITALGETVTTDLLYLRIYAPDCTVVGESAALLDFGTVWFRSLVVTNPAPGTYTVGIY
                     GRINTPTNYWGAFNTYVKN"
     gene            2740..>3012
                     /locus_tag="VM841_05770"
     CDS             2740..>3012
                     /locus_tag="VM841_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89726.1"
                     /translation="MARSRVLLLTALLISSCGGAGSAAVPVSTAAPAHVVTAVNKSGV
                     TAVPPETIKSGETVVELGDHWYAPAQLYVTVGTKVTWKQVGSQEHDV"
BASE COUNT          498 a         1078 c          987 g          449 t
ORIGIN      
        1 ctactacact gatgtcgtag atacgcgcgt cggaggctgc ggatgatccg gatcgcatcc
       61 gaagacgaca gcgctctcag ccgtcggcaa gccctgcggc tcggcttctt cggcatcgcg
      121 gcgctcgccg cgacggagct cgtcgcggcg ttcgctccct tcatgcgcgt cacgcgcttc
      181 gaagggctgg gcgcgaaggt ggcggtcccg ttcacgaagg ccgagatcct cgaacggttc
      241 gccgcgacga atgacgaccc catcctgttc gctcaggaca agttcttcct cctgcgccct
      301 ccggagggga tcatcgcggc gtaccggaag tgcacgcatc tcgggtgcgc cgtgcccttc
      361 gcgaagcatg aggaccggtt ccactgcccg tgccaccagt ccatctacga caagcggacg
      421 gcgctcctga tctcgggccc cgcgccgcgc ggcctcgacc tcttccacat gagcgaagcg
      481 gacgggaagc tcgtcgtaga cacgaacccg ttcgaactca tggtccgcga cgacaacaag
      541 tgggatcccg cccacgtcga ggtgagcggc tgacgctccg accgtgaatg gccgcactgg
      601 gggatgggtc agcgtccgcc gaggaccagg agcgcgtagg ccgcgcgccg gtgctcgacc
      661 cggtgcgccg tgcgctccat cccgacggtc atcttgccga cggccgccgg accgatcacg
      721 aacacgaggt cagaccgccg tcgcgcttcg gacacgtcga cagcctatcg agcgctctct
      781 tcggggaagg gccggatctt caccgatcgt tgaccgacgt ttgaccgggc gttgaccgcc
      841 cgttgaccgc ctcaggctca tccgttgccg aacatcacgt acgacaacgc aaatgagcag
      901 gaggaactcg tgaaacatcg cgcagggtgg ctccttaccg ccgttctcgt catcaccggg
      961 ctcgccgccg ggatcggcgc gcgcccggcg gacgcgagct ccgccgcagt gtccggtgcg
     1021 ctcgcgtcgt ggctcgcgac cgcgcccgcg gacgccggct tcaagaccat cgtcacgttc
     1081 cacgaccgca gcggcatgtc gcggctcgac gctctcgccg ccaaccagga cctcagcgtc
     1141 cttcccatgt cgttcgcggt cctcagcgcc gcgcagatca acaccgtccg cggatggccg
     1201 gaggtcgtca gcctctggca cgaccaggag aacgagctca tcctcgacga gagcgtcgcc
     1261 ctgatcggtg ccgacaaggt ccacgcgggg accaagctga aacggggcta caccgggcta
     1321 ggcgtcagcg tcgccgtcat cgacaccggc gtcgacgcct cccacccgga cctgaccggt
     1381 cgcgtggcga tgttccaggt agccggcgac gcgttcgacc gcgacggcgt cgccgtcctc
     1441 gccgcaccga cggccgacac gtacgggcac ggcacgcacg tctcgtcgac gatcaacgga
     1501 agcggcgccg cgagcgccgg ccgctaccag ggcgtcgcgc cgggatcgca ggtgtactcg
     1561 ttcaagaccg acgtcggcgc cttcctcctg agctcatggg ccctggcgtc cttcgactgg
     1621 gtcctcgcca acccgcaggc cggcatccga gtctcgagca actcgtgggg cacgatgggt
     1681 ccggacgact acgacccggc gaacccgatc aacgtgacca cgaagacgat gtacgaccgt
     1741 gggatcacgg tcatcttcgc ggccggcaac tctggcgggc cgaacacgct gaaccagtac
     1801 gcgaagtcgc cgtgggtcgt gagcgtcgcc gccggcgaca aggccgggaa gctcgcttcg
     1861 ttcagctcgc gcgggcggat cgacgacaac tgggaccgtc gcacggcaca gggcacgaac
     1921 accgggatct accgtccgac gatcaccgcg ccaggcgtga gcatcgaggc cgcgaagtcg
     1981 gcgcaggcga cgctcatggc gaccggcacc gaccccgcga atccgttcta cacgaccgcg
     2041 tccggcacga gcatggccgc gccgcacgtc gcgggcgtcg ccgcgctcat gctgcaggcg
     2101 cggccgggcc tcaagccgca gcacatcatc gacatcctcg aagggaccgc gacggacatg
     2161 cggagctacg agctgttcga ggcgggcatc ggctacctcg acgcgcacgc tgccgtcttc
     2221 gccgcggaga agggcagggt catcttcccg cccgcggtga gcggcaagac gcccacgttc
     2281 acgctcgcat cgacgacgcc gttcgaaggc agcgtcgata cgccgaacac gtgggcgctc
     2341 gcgaggtgca aggacgacct cggcgtgctc gatcaccacc tgttcgaggt ggcggccggt
     2401 gtgggcgcga tctacgccga gatcgagtgg ccgagcatca ccgccctcgg cgagaccgtc
     2461 acgaccgacc tgctgtacct ccgcatctac gcaccggact gcaccgtcgt cggcgagtcg
     2521 gcggcgctcc tcgacttcgg gaccgtgtgg ttccgctccc tcgtcgtgac gaacccggca
     2581 ccggggacgt acacggtcgg catctatggg cgcatcaaca cgccgacgaa ctactggggc
     2641 gcgttcaaca cgtacgtgaa gaactagccc agcaaggggc cctccttctc atcggtacgg
     2701 ggccgttccc gcgaggggac ggcccctatc gttccggcga tggctcgatc gcgcgtcctt
     2761 ctcctcaccg ccctgctcat ctcgtcgtgc ggcggcgccg gctccgccgc cgtgcccgtg
     2821 tcgaccgcgg ctccggctca cgtcgtgacc gcggtgaaca agagcggcgt caccgcggta
     2881 ccgccggaga cgatcaagtc cggcgagacc gtcgtcgagc tcggcgacca ctggtatgcg
     2941 ccggcgcagc tgtacgtgac cgtcggcacg aaggtgacgt ggaagcaggt cggctcgcag
     3001 gagcacgacg tg
//
LOCUS       DATLFO010000151         4067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_104921, whole genome shotgun sequence.
ACCESSION   DATLFO010000151 DATLFO010000000
VERSION     DATLFO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104921"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..610
                     /locus_tag="VM841_05775"
     CDS             197..610
                     /locus_tag="VM841_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVL89727.1"
                     /translation="MRSATEYLTFNTKTEHAYVNITGEVARIVEASGVKEGLCLVSAM
                     HITAGVYVNDDEPGFREDLTALLEHLAPSGQDYAHHRTGETNGEAHLRNMLIGHQVTL
                     PVTKGSLDLGPWEQVFYAEFDGRRRKRVVVKVIGE"
     gene            621..1166
                     /locus_tag="VM841_05780"
     CDS             621..1166
                     /locus_tag="VM841_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVL89728.1"
                     /translation="MDHEAARALVRDKCDKDITVRHLISVEGVMRRLARHFGEDEHRW
                     GLTGLFHDLDQDFTPNDPERHARLAAEWLKEAGVDESVINGVLAHAHPEYQTDLMSRS
                     IVQADALAGLLVAAALMRPEKANGMKVSSVKKKLKDKSFAPGVNRDEIRDCDKIGLDL
                     DTFVELGIEGLQIVAADIGLA"
     gene            1314..2321
                     /locus_tag="VM841_05785"
     CDS             1314..2321
                     /locus_tag="VM841_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVL89729.1"
                     /translation="MRKLMIAIATMAALAVALPSGAVPAPVGSVPVGAGPSAVAWNPV
                     TRLFYVANSSENAVSLVAAAGSRLNAGAPAPGQASAGAEIARIPVGSAPAGLAVHPET
                     GTVYVSNQGANSISAISASGLVQTGPVGAMPWGLAVDSATGKLCVAMFGDNKLWIIDS
                     ATMATSAVLSIPGGPNAVAAGGGRCYVTAFYAGTVTAVNLATPSIAWSALSVPGVIGS
                     CPSSVALYPGLPAHMYVTNECSNSLSIINPINGGLLLGAPVPLAPGAAPAGVAASDSS
                     VYVTERGLGRVSVLNPFTGTGVALPAGNSPRGVAVSDVPECALAANFSSQTASMICDV
                     L"
     gene            2598..3599
                     /locus_tag="VM841_05790"
     CDS             2598..3599
                     /locus_tag="VM841_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVL89730.1"
                     /translation="MRIRAGAVVTALAALLQPSAGNAAMTIVRTVSIAERGPAAIAFD
                     PITNLAWVGSVTDDAINVFAGVHSVAKITQAGLNPTGIAIDALGQRALVTSYGSNKVL
                     VYNTADRRKIASVDVAGGPWGVAVDPRDGTAYVATFAANVLTVVRRDNTTQQIALPGC
                     GGAIGVAYSVPASRVLVSCIYSGTVHVLDGATLATVNVIDIGLGAYTWGIAASLRDAK
                     VYAVNWSGGAVNTGTVTVIDASLAAAVATGFTGGAPIGVAVSPGGTPYVTLSATNELA
                     ILEPDSGLVGDRVALPVDPGDGQNQPHAVGVHPSGRYAIVGNFHAESVSFVAAAPHL"
     gene            3634..>4067
                     /locus_tag="VM841_05795"
     CDS             3634..>4067
                     /locus_tag="VM841_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89731.1"
                     /translation="MRRLRSLAAFLGAVGLLATAAALSAPSRAEAAWPATIFARDSIT
                     IAIVDGAAPDARGTDGTGPSLAGVGVYPGSRDAEAFLTQIGIGAPTGSVSGATGEAFG
                     LLRALGDGVAVETAGRRAPLVAFGAPSTPAGRSADEGRPVLR"
BASE COUNT          607 a         1406 c         1430 g          624 t
ORIGIN      
        1 acgcgaagat cggcagcgcc gtgatcttcg gcagcaactg gtgcggcgcc tcctcggtgg
       61 ccagctcgcg gcccaccagg atccgcttga tgatgctgcg ccgttgggtt ctctctcgta
      121 cggcaggcat ggccgccaga atacgcgcgc cgccgcggca aggcgacccg gcggggcggg
      181 ctcgtagact ggcgccatgc gatcggcgac cgagtacctg accttcaaca ccaagaccga
      241 gcacgcctac gtcaacatca ccggggaggt agcgcgcatc gtggaggcca gcggcgtcaa
      301 ggaggggctg tgcctggtct cggcgatgca cattaccgcc ggcgtatacg tcaacgacga
      361 cgagccgggt tttcgcgagg acctgaccgc gctgctggag cacctcgcgc cgtccggcca
      421 ggactacgcc caccaccgga ccggcgagac caacggcgag gcgcatctgc gcaacatgct
      481 gatcggccat caggtcacgc tgccggtgac caaggggtct ttggacctcg ggccatggga
      541 gcaggtcttc tacgccgagt tcgacggccg ccgccgcaag cgcgtcgtgg tcaaggtcat
      601 cggcgaataa ggggagcgag atggaccacg aagccgcgcg cgcgctggtg cgcgacaagt
      661 gcgacaagga catcaccgtc cgccacctga tctcggtcga aggcgtgatg cgccgcctcg
      721 cccggcactt cggcgaggac gagcatcgct ggggcctcac cgggctgttc cacgatctgg
      781 accaggactt caccccgaac gacccggagc gtcacgcgcg cctggcggcc gaatggctca
      841 aggaagccgg ggtggacgag tccgtgatca acggcgtgct tgcgcacgcg caccccgaat
      901 atcagaccga cctgatgagc cggtcgatcg tgcaggcgga cgcgctggcg ggcctgctcg
      961 ttgccgcggc cctgatgcgt cccgagaagg ccaacggcat gaaggtctcg agcgtgaaga
     1021 agaagctcaa ggacaagtct ttcgcgcccg gcgtgaatcg cgacgagatc cgcgactgcg
     1081 acaagatcgg ccttgacctg gacacgttcg tggaactcgg gatcgagggt ctgcagatcg
     1141 tggccgccga catcggcctc gcctgacttc gccgtttcat cgccggaaag gcggatgagc
     1201 cggttcagcc ggggaaatcg aacatctctc cgaagggcac ccggcctcgg cgctcgaacc
     1261 tgtcgggaca cgcgcgcgct gcgcgccggt tccgcctggg gggatttcga gtgatgcgaa
     1321 agctgatgat cgcgattgcg actatggccg cgctggccgt cgcgctgccg tccggagctg
     1381 ttcccgcacc cgtcggctcc gtgcccgtcg gggccggtcc gtcggcggtt gcctggaacc
     1441 cggtcacgcg tctgttctac gttgcgaact cgtccgagaa cgcggtctcg ctcgtggccg
     1501 ccgctggaag ccggctgaac gccggcgcgc ccgcgcccgg ccaggcctcg gccggtgccg
     1561 agatcgcgcg catcccggtg ggctcggcgc ccgcgggcct tgcggtgcat ccggagaccg
     1621 gcacggtgta cgtgtccaac cagggcgcca actcgatctc ggcgatcagc gcttccgggc
     1681 tcgtccagac cggtccggtc ggggcgatgc cgtggggcct tgccgtggac tccgcgaccg
     1741 gcaagctctg cgtggcgatg ttcggtgaca acaagctctg gatcatcgac tccgcgacga
     1801 tggcgacctc agcggtgctg tcgattcccg gcggccccaa cgccgtcgcc gcgggcggcg
     1861 gccgctgcta cgtcaccgcg ttctacgccg gcaccgtcac cgcggtgaac ctcgcgacgc
     1921 cgtcgatcgc gtggtcggcg ctgagcgtgc ccggcgtgat cggatcgtgc ccgagcagcg
     1981 tcgcgctcta ccccggcctg ccggctcaca tgtacgtgac caacgagtgc agcaacagcc
     2041 tgtcgatcat caacccgatc aacggcggcc tgctgctcgg cgcgccggtc ccgctcgcgc
     2101 ccggggccgc gcccgcgggc gtggcggcga gcgactcgtc ggtgtacgtg accgagcgag
     2161 ggctcggccg cgtcagcgtg ctcaacccgt tcaccggcac cggcgttgcg ttaccggcag
     2221 ggaactcgcc gcgcggtgtg gccgtgtcgg acgtgcccga gtgcgcgctg gccgcgaact
     2281 tctcgtcgca gaccgcctcg atgatctgcg acgttctcta gacgctccac ccaacccgac
     2341 caccggccct cggacgatct ccgagggccg gcccgttttc ggagcggggt caggttgtct
     2401 tgccgggcca gccgctctgc aacggcagcg gcgccgaggc gctccacccg ccgtcgagcc
     2461 ccggcggtcc ggggaatcga tcacggcggg aacctggaag aatcgaacca tccggaacgt
     2521 tcgtttctgc cggagggagg gatttccccc gaagaatcca aatcatcgct tgttcgcttt
     2581 gaacacgagg aggcgggatg aggattcgag cgggagcggt cgtgacggcc ctggcggcat
     2641 tgctgcagcc gtcggcgggc aacgcggcga tgacgatcgt gcgtacggtg tcgatcgccg
     2701 agcggggccc ggccgcgatc gccttcgacc cgatcacgaa cctcgcctgg gtcggaagcg
     2761 tgaccgacga cgcgatcaac gtcttcgccg gcgtgcattc cgtcgccaag atcacgcaag
     2821 ccggcctcaa cccgaccggc atcgcgatcg acgcgctcgg ccagcgggca ttggtgacct
     2881 cgtacggctc gaacaaggtg ctcgtctaca acaccgccga ccgtcgcaag atcgcctcgg
     2941 tggacgtggc cggcggaccg tggggcgtcg cggtggatcc gcgcgacggg accgcctacg
     3001 tcgcgacgtt cgccgcgaac gtgctgaccg tggtgcggcg cgacaacacc acgcagcaga
     3061 tcgcgttgcc cggctgcggc ggcgcgatcg gcgtggccta tagcgtgccc gcctcgcgcg
     3121 tgctcgtcag ctgcatctac agcggcaccg tgcacgtgct cgacggcgcg acgctggcca
     3181 ccgtgaacgt gatcgacatc ggcctgggcg cctacacgtg ggggatcgcc gcgagcctgc
     3241 gcgacgcgaa ggtctatgcc gtcaactgga gcggcggcgc cgtgaacacc ggaacggtca
     3301 ccgtgatcga tgcgagcctg gccgcggccg tcgcgaccgg cttcaccggc ggcgcgccga
     3361 tcggcgtggc ggtctcgccc ggcggcacac cctacgtaac gctgtcggcc acgaacgagc
     3421 tcgcgatcct cgagccggat tcgggcctcg tcggcgatag ggtcgcgctg ccggtcgatc
     3481 ccggcgacgg acagaaccag ccgcacgccg tcggcgtcca tccgagcggc cggtatgcga
     3541 tcgtcgggaa ttttcatgcc gaatccgtgt cgttcgtagc ggccgcgccg cacctgtaac
     3601 ggctcggccc gcccgccgcg tatcgttgcg tcgatgcgac gcctgcgaag ccttgccgcg
     3661 ttcctgggag ccgtcggatt gctcgcgacg gcagcggctc tgagtgctcc ctcgagggcc
     3721 gaagccgcgt ggccggcgac gatctttgct cgcgactcga tcacgatcgc gatcgtggac
     3781 ggcgccgctc cggacgcgcg cgggacggac ggcaccgggc cttcgctggc cggggtcggc
     3841 gtgtatccgg gctcacgcga tgcggaggcg tttctgacgc agatcggaat cggcgctccg
     3901 accggcagcg tgagcggcgc gacgggcgag gcattcggct tgctgcgggc gctcggcgac
     3961 ggtgtcgccg tcgagaccgc cggccggcga gcccccctgg tggcctttgg cgctccgtct
     4021 acgcctgcgg gccgcagcgc ggacgagggc cggccggtgc ttcgcat
//
LOCUS       DATLFO010000152         7289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_84516, whole genome shotgun sequence.
ACCESSION   DATLFO010000152 DATLFO010000000
VERSION     DATLFO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84516"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VM841_05800"
     CDS             complement(<1..864)
                     /locus_tag="VM841_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL89732.1"
                     /translation="MVNFALTEEQLEFQKWLHGFAETEIRPVASQYDESEEFPWEVVK
                     KAAEIGLYGFDIYAQGGADESGLMMPMLAEEIAWGCAGIGLAILGTGLPLAAVASSGT
                     PEQLQKWVPEMFGTPDNVKLGAYAVTEPGAGSDVSKLRSTAKRERDGWLLNGTKVFIT
                     NGGIADVHIVVATVDPELGHRGQASFIVGPNTPGFSQGKKEKKLGIRASHTAEIILED
                     CWVPNENLLGGEEKLQAKLERARSGKTTGRSGALATFEATRPLVGAQAVGIARAAWEF
                     ARDYAKDRTTFG"
     gene            1026..1622
                     /locus_tag="VM841_05805"
     CDS             1026..1622
                     /locus_tag="VM841_05805"
                     /inference="COORDINATES: protein motif:HMM:NF039596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL89733.1"
                     /translation="MAQRRLSAEARRAQLIAIAREVFVEDGYDGAGMEAIATRAGVTK
                     PVLYQHFESKKELYLALLGEDMAALQGRVEGALDSAKTNRERIERGLAAYFEFIEQNE
                     GSFHLLFRETLGSEPDFREVVERFRDSVAARIGKIIAEETSLPIQVSELLARGVMGMG
                     ESAAQWWLEGRSVDKREVLKDLSELAWRGLSGLPRRES"
     gene            1647..2411
                     /locus_tag="VM841_05810"
     CDS             1647..2411
                     /locus_tag="VM841_05810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL89734.1"
                     /translation="MAKNRAQQRAQAKRQMNKQQRRRQQKMRTIRIWSTVAGALALAL
                     VLFFVFRGDGGGTVSPGSPSATGPTAPTTESSSPCPGAPTKTCYKDPPPMVIDASKSY
                     TATMKTNMGDIVIDLNTRETPKTVNNFVFLAREGFYDGLVFHRVIPGFMIQGGDPAGN
                     GTGDPGYKFEDETKPTDSFDKPGLLAMANSGPNTNGSQFFITVDPATHLNGKHTIFGT
                     VTKGMDVVEKIVSVETGAQDRPVKPVTITTVEIAEK"
     gene            complement(2415..3344)
                     /locus_tag="VM841_05815"
     CDS             complement(2415..3344)
                     /locus_tag="VM841_05815"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HVL89735.1"
                     /translation="MKRLHALLLVAVVIGGVAGARVLLRDDAAGPGPAPLQEQPAEAT
                     GRTVPLEIAPLPPTLKKEGDFACAELRNQDGSFTGRAQGGSIRNVSGGSLTYAFTFEV
                     SAGEQTENVQSRFRLNDGQGIPSVTMVSKSVAGPVTKCTLRIVAAEVTAQDIAKVDAA
                     AEAAGCGRVMRHESDGHDHIEPPQRGSYSTSPPTSGNHYSAPSRTGVHDEPVPDEVQV
                     HNLEHGHVGLHYKGVSNAGRAALEDAARGDTTWIFAAPYPAMDAAVAMTAWTVSLHCA
                     TEPKDPAALAGLAELFVSAFRDQGRESIPGMPA"
     gene            complement(3341..6790)
                     /gene="metH"
                     /locus_tag="VM841_05820"
     CDS             complement(3341..6790)
                     /gene="metH"
                     /locus_tag="VM841_05820"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HVL89736.1"
                     /translation="MSGFLDLLRERIVVFDGAMGTQIHAADLSLDDYQGEEGNSEILN
                     FTMPDVVRSIHEAYFRAGADVVETNTFGANAVVQAEYDGADRVYDLNVAGARLAKEVA
                     SSFTDRPRFVAGSIGPGTKLPSLGHATYRELSGSYATQARGLIDGGADLLIVETCQDM
                     LQVKSALGGIMDAFDASGQRLPLIVQVTIETTGTMLLGTEIAAALAALEPYQIIDVVG
                     LNCATGPLEMTEHVRYLCQSSRRPVSVQPNAGLPELRDGKPFYPLTPGELVRYQRVFA
                     EEFGTSIVGGCCGTTPEFTAALRAAFDDATPKERTPVHEPACASLYVAQPFRQDTSFF
                     VIGERANAQGSRKFKEIVGAGDWDQTVQIAKEQVREGAHALDVCVDYTGRDGVEDMHS
                     IIERYRTQVTLPLFIDSTEPPVMEAALEMIGGRAVINSINLEEGDGQDARLRRNLRLA
                     QRFGAACVALAIDERGQATTADWKVEVCKRLRDVALEYGLAPHDLIFDTLTFPITTGM
                     EEQRRAAIETIESVKRIKEEIPGAFTSLGVSNVSFGLSPAARQVLNSVFLHECVQAGL
                     DAAIVSPRQILPAAKIPDEQREAALNLIYDRRAPGVDPLVEFTSLFEGVSTQRETGPD
                     LAELPLDERLKRRIIDAERDGLEADLDAALQQMSALDIINTILLDGMKVVGDLFGSGE
                     MQLPFVLQSAETMKASVAYLEPHMEKADDGGKGRIVLATVRGDVHDIGKNLVDIILTN
                     NGYTVHNLGIKQPISAIIEAAERERADAIGLSGLLVKSTVVMREDLEELNRRELHRYP
                     VLLGGAALTRGYVEHDLRELYRGSVFYGRDAFEGLHTMDAIMDAKRRGEPVAAPEPRR
                     ERPKRRTVQVSEDTTRSDVASDVPVPAAPFLGSRVVKGIGIPEVAKHLNRVALFKNQW
                     NYKRKRSQSDEEYQAFIDDKVEPVLRSLIDRAVAEQILTPAVVYGYYRAQSEGNDLIV
                     HREDGDPLRFTFPRQQRDRRLCIADFFRPVDSGQMDVVAFHVVTMGERVAKEAQTLFE
                     ADQYTDYLYLHGFGVEMAEALAEMWHRRIREELGITDQDGPSVEDWFDSRYRGARYSF
                     GYPACPDLEEQTKIFTLLEPGRIGVELSEEFQLHPEQSTSAIIVHHPEARYFSVGRPA
                     ITP"
     gene            complement(6914..>7289)
                     /locus_tag="VM841_05825"
     CDS             complement(6914..>7289)
                     /locus_tag="VM841_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89737.1"
                     /translation="FAGPATVTAGKSSQHPCRSAGPTSARVNVVAAKIGGVVVTSAPG
                     EIFANFSNTIEERSPITALAIGQANDALGYMPQSFESDHAARQGLGFVGKPYFEYEDA
                     YSIDACFGDMALETTLRLLGTL"
BASE COUNT         1178 a         2449 c         2442 g         1220 t
ORIGIN      
        1 gccgaaggtg gtgcggtcct tggcgtagtc acgcgcgaac tcccacgcgg cacgcgcgat
       61 gcccacggcc tgcgcgccga cgagcggccg cgtcgcctcg aacgtggcga gcgcgccgga
      121 tctgccggtg gtcttgccgc tgcgcgcgcg ttcgagcttc gcctgcagct tctcttctcc
      181 gccgagcaga ttctcgttgg gaacccagca gtcctcgagg atgatctcgg cggtgtgcga
      241 ggcgcggatg ccgagcttct tttccttctt gccctgcgaa aagcccggcg tgttcggtcc
      301 gacgatgaac gatgcctggc cgcgatggcc caattctggg tcgacggtcg cgacgacgat
      361 gtgcacgtcg gcgatgccgc cgttggtgat gaagaccttc gtgccgttga gcagccagcc
      421 gtcacgctcg cgcttggcgg tcgagcggag cttggacacg tcggatccgg cgcccggctc
      481 ggtcaccgcg tacgcgccga gcttgacatt gtcgggcgtg ccgaacatct ccggcaccca
      541 tttctgcagc tgctcgggcg tgcccgacga ggcgaccgcg gcaaggggaa gtccggtgcc
      601 gagaatcgcc aggccgatgc cggcgcaccc ccacgcgatc tcttcggcaa gcatcggcat
      661 catcagaccc gactcgtccg cgccgccttg cgcgtagatg tcgaaaccgt acaagccgat
      721 ctcggcggcc ttcttcacga cctcccaggg gaattcctcg ctctcgtcgt actgcgaggc
      781 gaccggacgg atctcggtct cggcgaagcc gtgcagccac ttctggaact cgagctgctc
      841 ttcggtgagc gcaaagttga ccatgacctg actccctgag agtcgttcgt tcgaatctgc
      901 ccgaacgacg cttatgttac ttgacggtaa cttaccaatg ggtaatatag gcgacggagt
      961 acgcttcgtc aacatcgggc cccgcaccgg gatgcccgtt tcggtgccgg gtgaaggagc
     1021 atgggttggc tcaacgacgc ctgtccgcgg aggcacggcg cgcgcagttg atcgcgatcg
     1081 cgcgcgaggt cttcgtcgag gacggatacg acggcgccgg catggaggcg atcgcgaccc
     1141 gcgccggggt gaccaagccg gtcctctacc agcatttcga gtccaagaag gagctttacc
     1201 ttgcgctgct gggcgaagac atggccgccc tgcaaggccg cgtcgaaggc gcgctggact
     1261 cggccaagac gaatcgggaa cgcatcgagc gcggcctggc cgcctacttc gagttcatcg
     1321 agcagaacga aggctcgttc catctgctgt tccgcgagac gctgggatcc gagccggact
     1381 tccgtgaggt cgtcgaacga ttccgcgact cggtcgcggc gcgaatcggc aagatcatcg
     1441 ccgaggagac gtcgctgccg atccaggtga gcgagctgct cgcgcgcggc gtcatgggca
     1501 tgggcgagtc cgcggcccag tggtggctcg agggtcgcag cgtggacaag cgagaagtgc
     1561 taaaagatct ctccgaacta gcctggcgcg gcctgtcggg gctgccgcgc cgcgaatcct
     1621 gatcgacatt cgaggaggct cttcaggtgg cgaagaaccg ggctcaacaa cgcgcacaag
     1681 ccaagcggca gatgaacaag cagcaacggc gacggcagca gaagatgcgc acgatccgca
     1741 tctggtccac ggttgcgggt gcgctcgcgc tggcgttggt gctcttcttc gtatttcgcg
     1801 gggacggcgg cgggaccgtc tcgcccggct cacccagcgc gaccggcccg acggcgccga
     1861 ccaccgagtc gtcgtcgccg tgcccgggag cgcccacgaa gacctgctac aaggatccgc
     1921 cgccgatggt catcgacgcg tcaaagtcct acacggcgac gatgaagacc aacatgggcg
     1981 acatcgtgat cgacctcaac accagggaga cgcccaagac cgtcaacaac ttcgtgttcc
     2041 tcgcccgcga aggcttctac gacggcctgg tcttccaccg ggtgatcccc ggcttcatga
     2101 tccagggcgg cgacccggcc ggcaacggca ccggcgatcc cggctacaag ttcgaggacg
     2161 agaccaagcc gaccgactcg ttcgacaagc ccggcctgct cgcgatggcg aactcgggcc
     2221 ccaacaccaa cggctcgcag ttcttcatca ccgtcgatcc ggccacgcac ctgaacggca
     2281 agcacacgat attcggcacg gtcaccaagg gcatggacgt cgtcgagaag atcgtgagcg
     2341 tcgagaccgg cgcgcaggac cgtccggtca agcccgtgac catcaccacg gtcgagatcg
     2401 ccgagaagta gcggctacgc cggcatgccg gggatggatt cgcggccctg gtcgcggaag
     2461 gcggagacga agagctcggc gagcccggcg agcgcggcag gatccttcgg ctcggtcgcg
     2521 cagtgcaacg agacggtcca ggccgtcatc gcgacggccg cgtccatcgc ggggtagggg
     2581 gcggcgaaga tccatgttgt gtcgccccgt gcggcgtcct cgagggccgc acgcccggca
     2641 ttgctaacgc ctttgtagtg cagcccgacg tgtccgtgct cgaggttgtg cacctggacc
     2701 tcgtcgggga ccggctcgtc gtgcacgccg gtgcgcgagg gggcggaata gtgattgccc
     2761 gacgtcggcg ggctcgtcga atacgagcct cgctgcggcg gctcgatgtg atcgtggcca
     2821 tcgctttcgt gacgcatgac gcggccgcag ccggcggcct cggcggccgc gtcgaccttc
     2881 gcgatgtcct gggccgtgac ctcggcggcc acaatacgca gcgtgcactt cgtcaccggg
     2941 ccggcgacgc ttttggacac catcgtcacc gacgggatgc cctgcccgtc gttgagccgg
     3001 aaccggcttt gaacgttctc ggtctgctct ccggcggaaa cctcgaaggt aaacgcgtag
     3061 gtgagcgagc ccccggagac gttacggatc gagccgccct gcgcgcggcc ggtgaacgag
     3121 ccgtcctggt tccgcagctc cgcgcaggcg aagtcgcctt ccttcttcag cgtcggcggc
     3181 agcggcgcga tctcgagcgg cacggttcgc ccggtcgctt cggcgggctg ctcctgcaag
     3241 ggcgcgggac cgggaccggc ggcgtcgtcg cgtagcagca cgcgcgcgcc ggcgacgccg
     3301 ccgatcacga cggcgacgag cagcagcgcg tgaagacgct tcacggcgtg atcgccggcc
     3361 ggccgaccga gaagtagcgg gcctcgggat ggtggacgat gatcgcgctg gtggactgtt
     3421 cgggatgcaa ctgaaactcc tcggatagct cgacgccgat ccggcccggc tcgagcagcg
     3481 tgaaaatctt ggtctgctct tccagatcgg gacaggcagg gtatccgaac gagtaccgcg
     3541 cgccgcgata gcgcgagtcg aaccagtctt cgacgctcgg cccgtcttga tccgtgatgc
     3601 ccagctcttc gcggatgcgg cgatgccaca tctcggcgag cgcctcggcc atctcgacgc
     3661 cgaagccgtg cagatacagg tagtcggtgt actgatccgc ctcgaacagc gtctgcgctt
     3721 ccttggcgac ccgctccccc atcgtcacga cgtggaaggc gacgacgtcc atctgacccg
     3781 agtccacggg gcggaagaag tcggcgatgc acagccgccg gtcgcgctgc tggcgcggga
     3841 acgtgaagcg cagcggatcg ccgtcctcgc gatggacgat cagatcgttt ccttcggact
     3901 gcgcgcggta gtagccgtag accacggcgg gcgtcagaat ctgctcggcg acggcccggt
     3961 cgatcagcga tcgcaagacc ggctcgacct tatcgtcgat gaaggcctga tattcctcgt
     4021 cgctttgcga gcgcttgcgc ttgtagttcc actggttctt gaacagcgcc acgcggttca
     4081 gatgcttggc gacctcgggg atgccgatgc ctttgacgac gcggctgccc aggaacggcg
     4141 cggcgggcac cggcacgtcg gaggcgacgt cgctccgggt ggtgtcctcg ctgacttgga
     4201 cggtgcggcg cttcgggcgt tcgcggcgcg gctcgggcgc agcgacgggc tcgccgcgac
     4261 gcttggcgtc catgatcgcg tccatcgtgt gcaggccttc gaaggcgtcg cggccgtaga
     4321 atacgctgcc ccggtagagc tcgcgcagat cgtgctcgac gtagccgcgc gtgagtgccg
     4381 cgccacccag aagcaccgga tagcggtgca gctcgcggcg gttcagctct tccaggtctt
     4441 cgcgcatcac gacggtggac ttcaccagca ggcccgacaa gccgatcgcg tccgcgcgct
     4501 cgcgctcggc ggcttcgatg atcgccgaga tcggctgctt gatcccgagg ttgtgcaccg
     4561 tgtagccgtt gttggtcagg atgatgtcca ccagattctt gccgatgtcg tgcacatcgc
     4621 cgcgcacggt cgccagcacg atccggccct tgccgccgtc atcggccttc tccatgtgcg
     4681 gctccagata cgcgaccgaa gctttcatcg tctcggccga ctgcagcacg aacggaagct
     4741 gcatctcgcc cgagccgaac agatcgccga ccaccttcat gccgtcgagc aggatcgtgt
     4801 taatgatgtc cagcgcgctc atctgctgca gcgccgcatc caggtccgct tcaaggccgt
     4861 cacgttcggc gtcgatgatg cggcgcttca aacgctcgtc gagcggcagc tcggccagat
     4921 ccgggccggt ctcccgctgc gtcgagacac cttcgaacag cgacgtgaac tcgaccagcg
     4981 gatcgacgcc gggcgcgcgc cggtcgtaga tcaggttcag cgccgcctca cgctgctcgt
     5041 ccgggatctt ggcggccggc aggatctgcc gcggcgagac gatcgcggcg tccaggcccg
     5101 cctgcacgca ctcgtgcagg aacacgctgt tgagcacctg ccgggccgcg ggcgacagcc
     5161 cgaacgacac gttcgacacg ccgagcgatg taaacgcgcc cgggatctct tctttgatgc
     5221 gcttgaccga ctcgatggtt tcgatcgcgg cgcggcgctg ctcttccatg ccggtcgtga
     5281 tggggaacgt gagcgtgtcg aagatcagat cgtgcggtgc gagcccgtat tccagtgcga
     5341 cgtcgcgcag ccgcttgcag acctcgacct tccagtccgc ggtggtcgcc tggccgcgct
     5401 cgtcgatcgc cagcgcgacg caggcagcgc cgaagcgctg cgcgaggcgc aggttgcggc
     5461 gcagccgcgc gtcctggccg tcgccttctt ccaggttgat cgagttgatg accgcgcggc
     5521 caccgatcat ctccagcgcc gcctccatca cgggcggctc ggtcgagtcg atgaacagcg
     5581 gcagcgtgac ctgcgtgcgg tagcgctcga tgatcgaatg catgtcttcc acgccgtcgc
     5641 ggccggtgta gtcgacgcac acgtccagcg cgtgcgcccc ttcgcggacc tgctccttgg
     5701 cgatctgcac cgtctgatcc cagtcgcccg cgccgacgat ctctttgaat ttgcgcgagc
     5761 cttgcgcgtt ggcgcgctcg ccgatcacga agaacgacgt gtcctgcctg aacggctgcg
     5821 cgacgtagag cgaggcgcag gcgggctcgt gcacgggcgt gcgctctttc ggggtcgcgt
     5881 cgtcgaaggc ggcgcgcagc gcggcggtga actcgggcgt ggtgccgcag cagccgccca
     5941 cgatcgaggt gccgaactcc tcggcgaaca cacgctgata ccgcaccagc tcgccgggag
     6001 tcagcggata gaacggcttg ccgtcgcgca gctccggcag gccggcattg ggctgcacgc
     6061 tgaccggacg ccgcgacgac tggcacaggt agcgaacgtg ctcggtcatc tcgagcggcc
     6121 cggtcgcgca attcaatccg acgacgtcga tgatctggta cggctccagc gccgccaacg
     6181 ccgccgcgat ctcggtgccg agcagcatcg tgccggtcgt ctcgatcgtg acctgcacga
     6241 tcagcggcag ccgctgcccg gacgcgtcga acgcgtccat gatcccgccg agcgcggatt
     6301 tcacctgcag catgtcctgg caggtctcca cgatcagcag gtctgcgccg ccgtcgatca
     6361 gcccgcgcgc ctgcgtcgcg tacgagccgg acagctcgcg gtaggtcgcg tgcccgaggc
     6421 tcggcagctt ggttcccggc ccgatcgatc cggccacgaa ccgcggccgg tccgtaaacg
     6481 aggacgcgac ctctttcgcc agacgcgcgc cggcgacgtt gaggtcgtag acgcgatcgg
     6541 cgccgtcgta ttcggcctgc acgaccgcgt tcgcgccgaa cgtgttggtc tcgaccacgt
     6601 ccgcgccggc ccggaagtag gcctcgtgga tcgagcgcac cacatccggc atggtgaagt
     6661 tcaggatctc gctgttgccc tcttcgccct ggtagtcgtc cagcgagagg tcggcggcat
     6721 ggatctgcgt gcccatcgcg ccgtcgaaca cgacgatgcg ctcgcgcaga agatccaaaa
     6781 agccgctcat gcggcggatt ctagcggcac gggtcgcggc ggcagtgact ccttcgatcc
     6841 gatcgaggac accagtctag aggacgccgc ggcacgaccc gcctcaatta ggcggagggc
     6901 atggctcccc cgcctacagg gtgccgagca accgcaaggt cgtctcgagc gccatgtccc
     6961 cgaagcaggc gtcgatcgag tacgcgtcct cgtactcgaa gtagggcttg ccaacgaagc
     7021 cgaggccctg ccgtgcggca tgatcggact cgaacgactg cggcatgtag ccgagcgcat
     7081 cgttggcctg cccgatcgcc agcgccgtga tcggcgagcg ttcctcgatc gtgttggaga
     7141 agttcgcgaa gatctcgccg ggcgccgacg tcaccacgac cccgccgatc ttggccgcca
     7201 ccacgttgac ccgggccgag gtcggccctg ccgagcggca cggatgctgc gagctcttgc
     7261 cggcggtcac ggtcgcggga cctgcgaac
//
LOCUS       DATLFO010000153         6221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_3281, whole genome shotgun sequence.
ACCESSION   DATLFO010000153 DATLFO010000000
VERSION     DATLFO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3281"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VM841_05830"
     CDS             <1..543
                     /locus_tag="VM841_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89738.1"
                     /translation="VLGGPISTLLTCAAKPAQVNNVEMGPPSTSDKLSPANTGHPNQK
                     RGEGNRFGLYYPVTDPIQAHWTTTGPTQGNGTGLIPATRGDHFNWRLSGTCATGEAFL
                     TQGTGIGYCGRSIGFGTGNVGPHSAIVRWESVGSQLITTDPSSAGTVNAQANPPGSAN
                     GSCLDGGASVFIVDGALVHM"
     gene            674..3598
                     /locus_tag="VM841_05835"
     CDS             674..3598
                     /locus_tag="VM841_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89739.1"
                     /translation="MGKKASLNTIRILLCLMLATGWMVAAPASGDSPVTVPERVGTLS
                     RFPKDIAKKYRFTDFRNQVYSQAPQGSIISNPDLGVLYQIWTVSGAHPTLADKSIIAE
                     RSLATLELRRTVVVPKAIMGSNGPTGIEFVHTLDERNQRLILEYGHYAIQNPDPFGIV
                     ILDLRTFALYMKAFPAGLVRNLEKSQAVFGLEYDEEGDRLIILAAGVDNNPSTTANTV
                     SLIGWDNAHLVVEGPLPGAPLMLGPRLVRNCRRDPINTVSTPRLTPIMIAKGPDLDSP
                     EEDPPVKRWVVFPCFTTPFSVNDVLVRISSENLFDPTAREEKALPAPASIAHWAMDVK
                     RGRMFLLNATQEVDAWVYEVASNAFIGLIEMSPKGDTSAASVALGVDERSGRLYGVSP
                     MGLGLMISEADQDPVPQADTYDVGGPLLASQATIAIDPARDRIIFVRGAGGPLSWVDD
                     YEVWKVPPPLPDQKPFDRDTLTTQVDEKEGRTTAEYGGVASGYGMRILMAGGLAGVPP
                     SNGNDSVGAFYATANVKCSQRSREIALGVVAETELSQSIVDARAESVRLDDNTVKDFG
                     QPSRCEPYYQYAGPVPQIFPQILRDNLFFTSTFGKTDQVRDHAGVSQAMRALGIDKAV
                     AELTGVDATGKSEDEFNKRTAPHTTWQYDAANCVADWYPNDRPGKSSERFTGETRVDC
                     RTPRSVSAQAEGHATAVLGPSGAAIPIKAGRATSETKVYLDPKRGLVAEATSRAENIR
                     IGTISIGFIENTARSFAKGRRGTAGTDEYRPVIGLVRGGGITGCELRCNIDQFLPQLN
                     NALAGRAEVRRINPDLRLKEGTDGGYEGGIIKSGKQRASDNALTGDDSREVPALELVI
                     YNDNPSLGRARQLVQFAGVRATSNYGIKLIAEGTPCPDCDQPPPPPPPEVGGVQVVTV
                     VQEVPGQTIIKRVPVVIGVPGGYRLLLANPRAAGGMMTVWLLLLSPALAYARRRRLKT
                     LG"
     gene            3662..5080
                     /locus_tag="VM841_05840"
     CDS             3662..5080
                     /locus_tag="VM841_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89740.1"
                     /translation="MAAFSALSFVAVQTPGQQQEQISAGDFDFGDQIVPTESAGPLSP
                     TGPAPTGGPTDGPAPTAGPATSKFQCAAGRNGGRTDKGVSANEIKVAATHVQSGLGSS
                     FLGSSNIGMRAVVSSVNSSGGICGRQIRLTLVDDGWDAARGSQYIRNFINDDYFALPV
                     VPSSEGLTAAIRNGTIDRAGIPVIGTDGMLKDQYQSPWVWPVATATVSTMRAMAFHAY
                     NKLGARNFGIVYDNQYKFGVEGATAFRDYVSSLPGAKVSAYVGIQPGQPSYTETTQFN
                     SKCGASKRCDFVAMLLEPGTATSWINSQGVDEQDKKLGFGTKGTGGAQPLFNENFARD
                     CGAPCSGMMLWTGYNPPIGALRNQPGIARYLSQVRAIDPGVDVNNQFLQGAYLGMQVF
                     VKALEDVGAELTRARLRAAMDSMTYATDLASSLTWKAGRHYANTSARAFLVVTASGGF
                     SGFRDAGTGFVPDPKPGVFPKG"
     gene            5090..5974
                     /locus_tag="VM841_05845"
     CDS             5090..5974
                     /locus_tag="VM841_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL89741.1"
                     /translation="MSDVLTALPLSLPLIAAYAIFAIGIVVIFRASKVLNIAHGAMAM
                     VPAYFTYEMTKRGIPMAAALPAGILSGALLGVLVERVFVRPLRSVGPTAQTVGTVAAL
                     GLMVAVVGKVWGTGTLPAPAVFPEGNLQIATSTLRYGQIGLLVVMVVVTVGLTALLQM
                     TDIGLAMRGAADNRRAAALMGVDPDVATTGAWILGGATAAIAGILLASVTLLQPLTLS
                     LQAIPAFVAALIGGLGSLYGAVVGALIVGLLTGIVPNIPAFAGVNGLQQVVPTLLAMI
                     VMAMRGQRYVASDIRSEL"
     gene            5971..>6221
                     /locus_tag="VM841_05850"
     CDS             5971..>6221
                     /locus_tag="VM841_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89742.1"
                     /translation="MSAAEAPELGGVSERPRAWRLAVFLAVVAFPYLPFVPFSVLGNV
                     NTAARYSMIGISIVVLTGWVGQISLGHAGFVGVGAYVTGL"
BASE COUNT         1054 a         2098 c         2016 g         1053 t
ORIGIN      
        1 gtgctgggcg ggcccatttc cacgttgttg acctgcgccg ctaagccggc gcaggtcaac
       61 aacgtggaaa tgggcccgcc cagcaccagc gacaagctga gtcccgccaa caccgggcac
      121 ccgaatcaaa agcgaggcga aggcaatcgc ttcgggctgt actaccccgt gacggacccg
      181 atccaggcgc attggacgac gacgggcccg acccagggca acggcaccgg tctgatccct
      241 gcgactcgcg gcgatcactt caactggcgc ctgtcgggca cgtgcgcgac cggtgaggcg
      301 ttcctgacgc agggaaccgg catcgggtac tgcggccgtt cgatcggctt cggcaccggc
      361 aacgtcggcc cccacagcgc gatcgtgcgg tgggagtccg tcggaagcca gctcatcacg
      421 accgacccga gctcggccgg cacggtcaac gcccaggcca acccgccggg gtccgcgaac
      481 ggctcgtgcc tcgacggtgg cgcttcggtg ttcatcgttg acggcgcgct ggtccacatg
      541 taacgacgaa attcggctgg gctgagtcgt gggaggagca ggcggacgag ccggctcctc
      601 ccatccggcc cggcacgcag catccggcga gagggtcccg catcggcacg cgcgggtcag
      661 aggtgggacg actatgggca agaaggcttc gctcaacacg attcggatcc ttctgtgctt
      721 gatgctggcc accggctgga tggtggccgc cccggcgagc ggcgatagtc cggtcaccgt
      781 ccccgaacgc gtcggcacgc tgtctcgatt ccccaaggac atcgccaaga aataccgctt
      841 caccgacttc cgcaatcagg tctacagcca ggctccgcag ggatcgatca tctcgaatcc
      901 cgacctggga gtgctgtatc agatctggac cgtatccggc gcacacccga cgctcgccga
      961 caagtcgatc atcgcggaac ggtcgctcgc caccttggag ctgcggcgga ccgttgtcgt
     1021 accgaaggcg atcatgggct cgaacgggcc gacgggcatc gagttcgtgc acaccctgga
     1081 cgagcgaaac cagcgtctga ttctcgagta cggccactac gccatccaaa atcccgatcc
     1141 cttcgggatc gtcatcctcg atctccgcac gttcgccctt tacatgaagg cgtttcccgc
     1201 cggcctcgtc cgcaatctcg agaagagcca ggcggtcttc ggtcttgaat acgacgagga
     1261 aggcgaccgg ctgatcattc tggccgccgg agtggacaac aacccctcga cgacggcgaa
     1321 caccgtctct ctgatcggtt gggacaacgc gcatttggtc gtcgaaggac cgttgccggg
     1381 cgctccgttg atgctcggcc cgcggttggt tcgcaactgc aggcgcgacc cgatcaatac
     1441 ggtctcgacc ccacggctca ctccgatcat gatcgcgaag ggcccggacc tcgactcgcc
     1501 ggaagaggat ccacccgtga aacgctgggt cgtcttcccc tgcttcacca cccctttctc
     1561 ggtcaatgac gtgctggtgc ggatcagcag cgagaacctc ttcgatccca ccgcgcgcga
     1621 ggagaaggcc cttcccgctc cggcttcgat cgcgcattgg gcgatggacg tcaagcgtgg
     1681 ccgcatgttc ctgctgaacg cgacccaaga ggttgatgcc tgggtctacg aggtcgcatc
     1741 caacgccttt atcggcctca ttgagatgag ccccaagggc gacacgtccg cggcatcggt
     1801 cgcgctcggc gtggacgagc gaagcggccg tctttacggc gtcagcccca tgggactcgg
     1861 cttgatgatc tcagaggccg atcaggatcc cgttcctcag gccgacacct acgatgtcgg
     1921 tgggcccctg ctggcctctc aggcgaccat cgccatcgat ccggcacgcg accggatcat
     1981 cttcgttcgg ggcgcgggcg ggccgttgtc gtgggtggat gactacgagg tctggaaggt
     2041 tccgccgccc ctgcccgatc agaagccctt cgaccgggac acgctcacca cgcaggtgga
     2101 cgagaaggaa ggaaggacca ccgcagaata cggcggggtg gcgagcggat acggaatgcg
     2161 catcctgatg gccggaggtc tcgccggcgt gccgccatcc aacgggaacg actcggtggg
     2221 agccttctat gccaccgcca acgtcaagtg ctcccagcgc agccgcgaga tcgcgctcgg
     2281 ggtcgtggcc gagaccgagc tgtcgcagtc gatcgtggac gctcgcgcgg aatcggttcg
     2341 tctcgacgac aacaccgtga aagacttcgg ccagccgtcc cgatgcgagc cctactacca
     2401 gtacgccggt cccgttcccc agatcttccc gcagatcctt cgggacaacc tctttttcac
     2461 ttcgacgttc ggcaagaccg atcaggttcg cgaccacgcc ggcgtgagcc aggcgatgcg
     2521 tgcgctcggg atcgacaagg ccgtcgccga gctcaccggg gtggacgcga cgggcaagtc
     2581 ggaagacgag ttcaacaagc ggaccgcgcc acacacgacc tggcagtacg acgccgcgaa
     2641 ctgcgtggcc gattggtacc ccaatgaccg gcccggcaag agctccgagc gcttcaccgg
     2701 cgaaacacgc gtggactgcc ggactcctcg ctccgtctcc gcgcaggcgg aagggcatgc
     2761 gaccgcggtg ctcggtccgt ccggagcagc gattccgatc aaggccggcc gggcgacctc
     2821 cgagacgaag gtctatctcg atcccaaacg cggcttggtc gcggaagcca cctcgcgcgc
     2881 cgagaacatc cgcatcggca cgatctcgat cggtttcatc gagaacacgg cgcggtcgtt
     2941 cgccaagggc aggcgaggta cggcgggaac cgacgagtat cgtcccgtca tcggcctcgt
     3001 acgaggtggc ggaatcaccg ggtgcgagct gcgatgcaac atcgatcagt tcctgcccca
     3061 gctcaacaac gcgctggcgg gacgtgccga ggtgcggcgc atcaatccgg acctgcgtct
     3121 caaggaggga actgacggcg gctatgaggg cggcatcatc aagtcgggca agcagcgcgc
     3181 ctcggacaac gctctaacgg gcgacgacag ccgcgaggtc cccgcgctcg agctggtcat
     3241 ctacaacgac aacccgtcgc tcggacgcgc acgacagctc gtccaattcg cgggggtccg
     3301 tgccacgtcg aactacggca tcaagctgat cgccgaggga acaccctgcc ccgactgcga
     3361 ccagcctccg ccgccgcctc cgcccgaggt aggcggcgtc caggtggtca cggtcgttca
     3421 ggaggtaccg ggccagacga tcatcaagcg cgttccggtc gtcatcggcg tgcccggcgg
     3481 atatcgactc cttctcgcca acccgcgcgc ggccggcggc atgatgaccg tatggctgct
     3541 ccttctttcg cccgcgctcg cctacgcgcg tcgcaggcgg ctaaaaacgc taggctaacg
     3601 cccgcaacct aggaatcgag cttcgaaaat gactccagga ctgagaaact tcatcgcagc
     3661 cgtggcggcc ttttccgccc tttcgttcgt cgccgtccag acccccggcc agcagcaaga
     3721 acagatctcg gccggggact tcgacttcgg cgaccagatc gtgccgaccg agtccgccgg
     3781 gccgctgtcg ccgaccggac cggctcccac aggcgggccg acggacggac cggctcccac
     3841 cgccggaccg gctacgtcga agttccagtg cgcagcggga cgcaacggcg ggcgcaccga
     3901 caagggcgtg tccgcgaacg agatcaaggt cgccgcgacc cacgtgcagt ccgggctggg
     3961 ctcgtccttc ctgggttcgt cgaacatcgg catgcgtgcg gtggtgtcgt cggtcaactc
     4021 gtcgggcggc atctgcggac ggcagattcg cctgacgctg gtggacgacg gatgggatgc
     4081 ggcgcgaggc tcgcagtaca tccgcaactt catcaacgac gactacttcg ctttgccggt
     4141 cgttccttca tcggaggggc tgacggccgc catccgtaac ggcaccatcg accgggccgg
     4201 cattcccgtg atcggaaccg acgggatgct caaggaccaa tatcaatcgc cgtgggtctg
     4261 gccggtcgcg accgctaccg tctcgacgat gcgcgcgatg gccttccacg cctacaacaa
     4321 gctcggggcg cggaacttcg gtatcgtcta cgacaaccag tacaagttcg gcgtcgaggg
     4381 tgcgaccgcg ttccgagact acgtgagctc gctgccgggt gcgaaggttt cggcctacgt
     4441 cggcatccag ccggggcagc cctcctacac cgagaccact cagttcaaca gcaagtgcgg
     4501 ggcgtcgaag cgctgcgatt tcgttgcgat gcttctcgaa ccgggaacgg cgacgtcctg
     4561 gatcaacagc cagggcgtgg acgagcagga caagaagctg ggattcggca cgaagggaac
     4621 cggtggcgcg cagccgctgt tcaacgagaa cttcgctcgt gactgcggcg ctccgtgctc
     4681 gggcatgatg ctgtggaccg gctacaaccc gccgatcggt gcgttgcgca accagcccgg
     4741 catcgcccgc tatctcagcc aggtccgggc cattgatccg ggagtcgacg tcaacaacca
     4801 gttcctccag ggggcctatc tcgggatgca ggtgttcgtg aaggctctcg aggacgtcgg
     4861 agccgaactc acccgggcgc gccttcgggc agcgatggac tcgatgacct atgcgacgga
     4921 tcttgcctcc tcgcttactt ggaaggccgg gcggcactac gccaacacgt cggcccgcgc
     4981 gttcctggtc gtcaccgcct cgggtggatt cagcgggttc cgtgacgcgg ggaccggctt
     5041 cgttcccgac ccgaagcccg gcgtgttccc caagggctga ccgaccacga tgtccgacgt
     5101 ccttacggct ctgccgctct cgctgccgct gatcgctgcc tacgcgatct tcgccatcgg
     5161 gatcgtggtg atcttccgcg cgtccaaggt gctcaacatc gcgcacggtg cgatggcgat
     5221 ggtgccggcg tacttcacct acgagatgac caagcggggc atcccgatgg ccgccgccct
     5281 gccggcagga atcctttccg gagcgctgct gggcgtgctc gtcgagcgcg tcttcgttcg
     5341 tccgcttcgc tccgtcggtc cgacggccca gacggtggga accgtcgcgg cgctcggact
     5401 gatggtggcc gtcgtcggga aggtctgggg cacgggcacg cttccggctc ccgcggtgtt
     5461 cccggaaggc aacctgcaga tcgcgacttc caccttgcgt tacggccaga tcggcctgct
     5521 ggtcgtcatg gtcgtcgtga cggtcgggct caccgcgttg ctgcagatga ccgacatcgg
     5581 actggcgatg cggggcgcgg ccgacaaccg gcgcgcggcc gctctgatgg gcgtggatcc
     5641 cgacgtggcc acgacgggcg catggattct cggcggcgcg accgcggcga tcgcgggcat
     5701 ccttctcgcc tcggtgacgc tgcttcagcc gctcaccttg tcgcttcagg cgatccccgc
     5761 gttcgttgcc gcgctcatcg gcggcctcgg cagcctgtac ggagcggtgg ttggtgccct
     5821 gattgtcggg ctgctcaccg ggatcgttcc caacatcccc gcattcgccg gagtgaacgg
     5881 actgcagcag gtcgtgccga cgctgctcgc catgatcgtc atggccatgc ggggacagcg
     5941 ctacgtcgct tccgacatcc ggagcgagct gtgagcgcgg cagaagcgcc ggagctcggc
     6001 ggcgtttccg agcggccccg cgcctggcgg ctggccgtct tcttggcggt cgtcgcgttc
     6061 ccctacctgc ctttcgttcc gttctcggtg ctcggcaacg tgaacacggc cgcccgatac
     6121 tcgatgatcg ggatctcgat cgtggtgctc accggctggg tcggacagat ctcgctcgga
     6181 cacgccggct tcgtgggggt cggcgcgtat gtgaccgggc t
//
LOCUS       DATLFO010000154         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_12974, whole genome shotgun sequence.
ACCESSION   DATLFO010000154 DATLFO010000000
VERSION     DATLFO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12974"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3026)
                     /locus_tag="VM841_05855"
     CDS             complement(<1..3026)
                     /locus_tag="VM841_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89743.1"
                     /translation="MKRRHRRLAYLVALAAIAGLLPQAARAATISVNCLNQADDLLAV
                     QAAVQSASSGDTIQLSGVCDFTTAAPHGGDATSIEAGAILVRPAAISNLTIEGADANA
                     TVIGNGLSAAFVIGPAAINTTIRRLRFVNFARSVVAINTTGTTVGADDGALPSANGLR
                     IVASATADSSIQGLAVSRNPESAPLGNVTVKYGANGMFTYTFPGQAAASLTNFRVAGN
                     YITYAPPGAGVPDVAAIDVRQRFYRMVNGVRIANNAVGMLTPTFLTTNVAAIKLHSTS
                     PADPVTNTIADYLIRNVDISNNNLGRIEELGNPLADINAAGRVGILLIGVANFNVIGN
                     GIRTVQTPTVVPMPGGGIIASDSRFGRIAGNGINTIATHEGETSDFGGIAVVDEFVSA
                     FDGAPKPQASADIVIEDNLLGTNAPGPGINGRGIVAAGSTRLTVRNNTTELTLQEALA
                     IAPVLEGPGSLSTPGPIPLPATVTNSVFCGNVLDGVVDDPNEVSYGEGGGTSTNNTYP
                     GGTLPANAPCPPSVPTLIFVPTDNTPPKVYEEGETSDSVSIRLSEPPAADVTVTMTTD
                     AQTTALTASLTFTPATWHAYQWAMARAIDDLVVEGTHFSTITFTFSSADPRWNTAPAV
                     TTPFIVIDNDLGLVDITPQFLNIGESGPNGTYSVVLTEAPTADVLVNFHPDTQLFVAE
                     HSIKFTPLNWNVPRIISVVAVDDIIREGTPHFGTITHSTTSSQPRFDQAAVPSVVASI
                     TDNDNPDSPSILAPGGGSCTSAGSVAFSGRGEEGALVELYENNVLIGSATVVGGLWAS
                     AMIPFGPGQHTVSARQIGSDGLIGRQWASITFTVDTVAPAAPVITSPADGATLTGNPH
                     TIEGTAEPYARVQVRRNGFPVALVTASASGSWSASIFLPAGPQALDAIAFDCALNASA
                     PSGTVNVDSAGVTEPVIVTPAEGAITRATVLVTGFGPAGATLIVKEAGRELRRTTVTA
                     SGDWSLTLTGLSGGEHKITATATAGGYAETSPVRTF"
     gene            complement(3223..>4453)
                     /locus_tag="VM841_05860"
     CDS             complement(3223..>4453)
                     /locus_tag="VM841_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89744.1"
                     /translation="VAGAGPPVPFRSIARDGLRSGYGSGDDRVETLYAPLTVSGRALG
                     VLGLGISRSAAVPGGSTRALALGVLVAAAVLLALAIGWRASRAISVPVGDLVAATKAL
                     EQGELATRAESDSRDEIGRLAESFNVMAAELQASHEELERKVAERTAELERVNAELAR
                     VSEAKSQFLATMSHELRTPLNAVIGFADFLSDPAFGPPSPKDVRKIAQDIHASGTHLL
                     TLINDLLDLAKIEAGRVEFDLRPVRLRDVARDVEAVCRPLAAAKDIRLVVKVPARLPD
                     VTADPGRVRQVLLNLIGNAIKFTPAGGTVTVTAAEEASLVAVSVTDTGPGMTGEEAAT
                     IFEPFERTSKARGIEGAGLGLALARRFVEIQGGTIDVRTAPGKGATFRFTLPRTRAAA
                     ARASRRPRRTTDERAFA"
BASE COUNT          652 a         1463 c         1633 g          705 t
ORIGIN      
        1 atgaacgtgc gcaccggcga ggtctcggcg tagccgcccg cggtcgccgt cgccgtgatc
       61 ttgtgctcgc cgccggagag gccggtcagg gtcagcgacc agtcacccga ggccgtgacc
      121 gtcgtgcggc gaagctcgcg gcccgcctcc ttgacgatca gcgtcgcccc ggccggcccg
      181 aaaccggtca cgagcacggt cgcgcgcgtg atggcgccct cggcgggcgt gacgatgacg
      241 ggctcggtga cgcccgcgct gtcgacgttg accgtgccgg acggcgcgct cgcgttgagc
      301 gcgcagtcga aggcgatcgc gtcgagcgcc tgcgggccgg cgggcaggaa gatgctcgcg
      361 ctccagcttc cgctggcgct cgccgtcacc agcgccacgg ggaacccgtt gcggcgcacc
      421 tgtacccgcg cgtacggctc ggccgtgcct tcgatggtgt gcgggttgcc ggtcagcgtc
      481 gccccgtcgg ccggcgaggt gatcaccggc gcggcgggcg cgaccgtgtc cacggtgaac
      541 gtgatcgatg cccactgtcg cccgatcagg ccgtcgctgc cgatctggcg ggccgagacg
      601 gtgtgctggc cgggaccgaa cgggatcatg gcgctggccc agaggccgcc gaccacggtc
      661 gcgctaccga tcagcacgtt gttctcgtac agctcgacga gcgcaccttc ctcgccgcgg
      721 ccgctgaagg cgaccgagcc ggcgctggtg cacgagccgc cgcccggcgc gaggatcgac
      781 ggactgtcgg ggttgtcgtt gtcggtgatc gacgcaacca cgctcggcac ggccgcctga
      841 tcgaagcgcg gctggctgct ggtggtcgag tgcgtgatcg tgccgaagtg cggcgtccct
      901 tcccgaatga tgtcgtctac cgcgacgacc gagatgatcc gcggcacgtt ccagttgagc
      961 ggcgtgaact tgatcgagtg ctcggccacg aacagctgcg tgtccggatg gaagttcacg
     1021 agcacgtcgg ccgtgggcgc ctcggtcagc acgaccgagt aggtgccgtt cggcccggac
     1081 tcgccgatgt tcaggaactg cggggtgatg tccaccagcc cgaggtcgtt gtcgatcacg
     1141 atgaacgggg tggtgaccgc cggcgcggtg ttccaccgcg gatccgccga cgagaacgtg
     1201 aaggtgatcg tgctgaagtg cgtgccctcg acgaccaggt cgtcaatcgc gcgggccatc
     1261 gcccactggt aggcgtgcca cgtcgcgggc gtgaacgtca gcgaggcggt cagcgccgtg
     1321 gtctgcgcgt cggtggtcat ggtgacggtg acgtcggccg cgggcggctc ggacagacgg
     1381 atcgagaccg agtcgctcgt ctcgccttcc tcgtagacct tcggcggcgt gttgtcggtg
     1441 ggcacgaaga tcagcgtggg aaccgacggc ggacacggcg cgtttgcggg aagcgtcccg
     1501 cccggatagg tgttgttcgt cgaggtgccg ccgccttcgc cgtaggagac ctcgttggga
     1561 tcgtccacga cgccgtcgag cacgttgccg cagaacaccg agttggtcac ggtcgcgggc
     1621 agcgggatcg gaccgggagt cgacagcgag ccggggcctt ccagcaccgg cgcgatcgcg
     1681 agcgcctcct gcaaggtcag ctcggtggtg ttgttgcgca cggtcaggcg ggtcgagccg
     1741 gcggcgacga tgccgcggcc gttgatgccc ggcccgggcg cgttcgtccc gagcaggttg
     1801 tcctcgataa cgatgtcggc gctcgcctgc ggcttgggcg cgccgtcgaa ggcgctgaca
     1861 aactcgtcca cgacggcgat gccgccgaag tcggaggtct cgccctcgtg ggtggcgatg
     1921 gtgttgatcc cgttgccggc gatgcggccg aagcgcgagt cggacgcgat gatgccgccg
     1981 cccggcatcg gcacgacggt cggcgtctga accgtgcgaa tgccgttgcc gatgacgttg
     2041 aagttggcga ccccgatcag caggatgccg acacgcccgg ccgcgttgat gtcggcgagc
     2101 gggttgccga gttcttcgat gcggccgaga ttgttgttcg agatgtcgac gttgcggatc
     2161 aggtagtccg cgatcgtgtt ggttaccgga tcggccggcg aggtcgagtg aagcttgatc
     2221 gccgcgacgt tggtggtgag gaacgtcggg gtcagcatgc cgaccgcgtt gttggcgatc
     2281 cgaacgccgt tgaccatccg gtagaagcgc tgccgcacgt cgatcgcggc cacatcggga
     2341 acgcccgcgc ccggcggcgc gtacgtgatg tagttgcccg cgacccggaa gttggtcagc
     2401 gatgcggcgg cctggccggg gaacgtgtag gtgaacatgc cgttcgcgcc gtacttcacg
     2461 gtcacgtttc cgagcggcgc cgactcgggg ttacgcgaca ccgccagacc ctggatcgag
     2521 gagtcagccg tcgccgacgc cacgatgcga aggccgttgg cggacggcag cgcgccgtcg
     2581 tcggcgccga cggtggtgcc ggtggtgttg atcgcgacga ccgagcgcgc gaagttcacg
     2641 aagcgcaggc gccggatcgt cgtgttgatc gcggccgggc cgatcacgaa cgcggccgac
     2701 aggccgttgc cgatcacggt cgcgttggcg tcggcgcctt cgatggtcag attgctgatc
     2761 gcggcggggc gaaccaggat ggcgcccgcc tcgatcgagg tggcatcccc gccgtgcggc
     2821 gccgcggtcg tgaagtcgca gacgccggag agctggatgg tgtcaccact cgacgccgac
     2881 tgaacggcgg cttgcaccgc gagcaggtcg tcggcctggt tgagacagtt gaccgaaatg
     2941 gtggcggcgc gcgctgcctg aggcagcaga cccgctatcg ccgcgagcgc gacgagatac
     3001 gcgagccgtc gatgtcttct cttcatatga atcccccctg gatcaattcc ctggatatcc
     3061 caaaaatact gtattttccc gcctgtccgg gattggggtc aatgaaccaa gcgttaagag
     3121 aattcgggag gattcagtac tccgtagacg cggagaggcc gggcaagcgc gcacagtgag
     3181 tgacccgtgt cgggttcggg gccgccgccg accgtgggcc gtctaggcga aggcgcgctc
     3241 gtccgtcgtc cggcgcggac gccgcgacgc tcgcgcggcc gctgcccggg tacgaggcag
     3301 cgtgaaccgg aacgtcgcgc ctttgccggg cgcggtacga acgtcgatcg ttccgccctg
     3361 gatttcgacg aagcgccgcg cgagcgcgag cccaagcccg gcaccttcga ttccgcgggc
     3421 cttggacgtg cgctcgaagg gctcgaagat cgtggcggcc tcctcgccgg tcattcccgg
     3481 accggtgtcg gtcaccgaca cggccacgag cgaagcctct tcggccgcgg tcaccgtgac
     3541 cgtgccgccc gccggcgtga acttgattgc gttgccgatc aggttgagca gcacctgacg
     3601 gacccggccc gggtcggcgg tcacgtcggg caggcgcgcc gggaccttca ccacgagccg
     3661 gatgtctttg gcggcggcca gcggacggca gaccgcctcg acgtcgcggg cgacgtcgcg
     3721 cagccgcacc ggccgcaggt cgaactcgac gcgtccggct tcgatcttgg ccaggtcgag
     3781 cagatcgttg atgagcgtga gcagatgcgt gccggacgcg tggatgtctt gcgcgatctt
     3841 gcgcacgtcc ttgggcgagg gcggcccgaa ggcgggatcg gacaggaagt ccgcgaagcc
     3901 gatcacggcg ttgagcggcg ttcgaagctc gtgcgacatg gtcgcgagga actgcgactt
     3961 cgcctcgctc acccgtgcga gctcggcgtt gacgcgctcg agctcggcgg tgcgctcggc
     4021 gacctttcgt tccagctctt cgtgcgaggc ctgaagctcg gcggccatga cgttgaacga
     4081 ctcggccagc cggccgatct cgtcgcgcga gtcgctctcg gcccgcgttg ccagctcgcc
     4141 ctgctcgagc gccttcgtag cagcgaccag atcgccgacc ggcaccgaga tcgcgcgcga
     4201 ggcgcgccat ccgatcgcga gcgcgagcag caccgccgcc gcgaccagca cgccgagcgc
     4261 aagcgctcgg gtggatccgc ccggcaccgc cgccgagcgc gagatgccga ggccgagcac
     4321 gccgagcgcg cggcccgaga ccgtcagcgg cgcgtagagc gtctcgacac ggtcgtcgcc
     4381 gctgccgtag ccgctgcgca gcccgtcacg cgcgatggaa cgaaacggaa ccggagggcc
     4441 cgcgcccgcg acc
//
LOCUS       DATLFO010000155         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_73054, whole genome shotgun sequence.
ACCESSION   DATLFO010000155 DATLFO010000000
VERSION     DATLFO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73054"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..807
                     /locus_tag="VM841_05865"
     CDS             58..807
                     /locus_tag="VM841_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89745.1"
                     /translation="MINNGSADRADEKSTWTQPGFVAAAAVVAIIVLLGIILAFTGGS
                     GSDALAPTTPPRPPVSGTAPAADPNASACDLPPGSQSVPTATPRTQWELVGQVAAPAA
                     PKTVGPAKVERGLRSCFAHSPKGALFAAVNVIAMTADPKRREIFVRNLTVPGIGRDRA
                     LAQRSSDVPNASTALQVVGFQIRDYRRDSAVVDLAFRVDTGNQAGTVHLPLAMRWLDG
                     DWKLALPDTGQPFDGMARLPSTDGYIAWKGA"
     gene            809..2185
                     /locus_tag="VM841_05870"
     CDS             809..2185
                     /locus_tag="VM841_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89746.1"
                     /translation="MAEHDCGLNPICHAGDLVTGAIGGVAGSAIDALADAVTEAVGKA
                     VASVGTLWVKVGTPNLTTTDGGTTASDSVGFLQGSLWWYMAAAAVLSVIIGGAKMAWE
                     GRAEPGRELLKSLMTLVVVAGAGLTAISLAVAAADGFAKWIIDSSLNGTDFGTNITAL
                     LGLSAITGLGAIIVIVLGLAAFVASLIQIALMVVRGGMLVILTGILPMSAAATNTEWG
                     RSWFKKCIAWLVAFILYKPAAAIVYATAFRLSGSDVFGGDELMSAIAGLVLMVLALVA
                     LPALMRFVTPMVGAMAAGGGAAGGAMAAGAIAAMPTGAMRLPRGGGSNGAGASAGPSG
                     GSGPSGATGVTGGRGPAGSNGSSSGGGSSNGGGGSPSPAAPAGAAASAPGAGASATAA
                     APAAGGAAAGGGAAASGAAAGAPAGPAGAAAGAAAAAAAGAQAAKGAAQGAAESQTGD
                     EGPSGSSR"
     gene            2169..>3274
                     /locus_tag="VM841_05875"
     CDS             2169..>3274
                     /locus_tag="VM841_05875"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HVL89747.1"
                     /translation="MEAAVEPKVRTYGNWRKPMSAGLGQLGLIGTGLMLVSLIIVILT
                     VGFFGLLTAAVVGLILTVMLGTLVIHDRHGKTVLQRMAAWIGWRRTRSRGAHLYRGGP
                     LGRNPWGTFHLPGLAARSTLSEARDSYDRPFALLHMPTTNHYTVVFGTEPDGAQLVDQ
                     SQIDVWVAHWGEWLASLADEPGLVAASVTIETSPDSGARLRREVLSRLDPAAPALAQE
                     MLHEVVETYPAGSATVKAYVALTFSATSRAGGKRRDEHEVTRDLAARLPGLTAGLTGT
                     GAGAARPLSAQQLCEAIRIAYEPRAAALIDESYDAGTVPELDWSDVGPSATEARWDTY
                     RHDGATSVTWSMTAAPRGGHRPRHRCRAVVPVGVP"
BASE COUNT          461 a         1132 c         1187 g          494 t
ORIGIN      
        1 gacccagggc agccgccaga cgcgaggcac accgccggcg acgaccggag catccggatg
       61 atcaacaacg gaagcgcaga cagggccgac gagaagagca cctggacgca gcccggcttc
      121 gttgctgccg cggccgtcgt cgcgatcatc gttctcctcg gcatcatcct cgcgttcacc
      181 ggcgggtcgg gcagcgatgc attggctccc acgactccgc ctcgaccgcc ggtttcgggg
      241 accgcgccgg ccgccgatcc caatgcgagc gcatgcgatc tcccgccggg atcgcagagc
      301 gtcccgacgg ccacgccacg cacacaatgg gaactggttg gccaagtcgc ggctcccgct
      361 gctccgaaga cggtcggtcc ggcgaaggtc gagcgcggac tgcgctcatg cttcgctcac
      421 tcgccgaagg gggcgctctt tgcggccgtg aatgtgatcg cgatgaccgc ggatcctaag
      481 cgccgggaga tcttcgttcg gaacctcacc gtgccaggca tcggccgcga ccgcgcgctg
      541 gctcagcgtt cgtccgatgt gccgaacgcg tccaccgcgc tgcaggttgt cggcttccag
      601 atcagggact accgcaggga ttcggcagtc gtcgacctgg ccttccgcgt tgacaccggc
      661 aaccaagcag gcaccgtcca cctacccctc gccatgcggt ggctcgacgg tgactggaag
      721 ctcgcgctcc cggacaccgg ccaaccattt gacggtatgg ctcgcctgcc cagcaccgac
      781 gggtacatag cttggaaggg cgcctgacgt ggccgagcac gactgcggcc tcaacccgat
      841 ctgtcacgcc ggtgacctcg tcaccggcgc catcggcggt gtggccggca gcgcgatcga
      901 cgccctcgcg gacgcggtga cagaggccgt tggcaaggcc gttgcctcag tcggcaccct
      961 gtgggtcaag gtcggaacgc cgaatctgac gacgacggat ggtggcacca ccgcgtcaga
     1021 ttcggtcggg ttcctacagg gttcgttgtg gtggtacatg gccgcggccg ccgtgctgtc
     1081 ggtcatcatc ggcggcgcaa agatggcgtg ggagggacgg gcggagcccg gccgggagct
     1141 gctcaagagc ctgatgacgc tcgtggttgt cgccggcgcc ggtctgactg cgatctcgct
     1201 cgctgttgct gccgctgacg ggttcgcgaa gtggatcatc gacagctcgc tgaacgggac
     1261 cgacttcggc acgaacatca cggcgctgct cggcctgtcg gcgatcacag gcctgggcgc
     1321 gatcatcgtg atcgtgctcg gcctggcggc cttcgtcgcc tcgctcatcc agatcgcgct
     1381 gatggtcgtg cgcggcggga tgctcgtgat cctcaccggc atcctgccga tgtccgcagc
     1441 tgccacgaac acggagtggg gccgctcctg gttcaagaag tgcatcgcgt ggctggtcgc
     1501 gttcatcctc tacaagcccg ccgcggcgat cgtctacgcg accgcgttcc ggctctcggg
     1561 ctccgacgtg ttcggcggcg acgagctgat gagcgcgatc gcggggctcg tcctcatggt
     1621 gctcgcgctg gtggcgctcc cagcgctcat gcgcttcgtc acgccgatgg tcggcgcgat
     1681 ggccgcgggc ggcggggccg ccggcggcgc gatggccgcg ggcgcgatcg ccgcgatgcc
     1741 gaccggtgcc atgcgcctgc cgcgaggagg cggctctaat ggcgcgggag cgtcggcggg
     1801 accctcggga ggctcgggtc cgtccggtgc cacgggagtg accggcggcc gtgggccggc
     1861 gggctccaat ggatcgtcgt ctggcggcgg gtccagcaac ggcggcggtg gcagcccgtc
     1921 gcccgctgcg ccggcgggtg cggccgcgtc tgcacctggc gcaggagcga gcgccaccgc
     1981 ggcggcgcct gcggccggtg gcgcagcggc gggtggtggc gcagccgcga gcggcgccgc
     2041 agccggagcg cccgcaggtc cagccggcgc ggccgcgggc gcagccgcgg ccgcggccgc
     2101 gggcgcacag gccgccaagg gcgctgcgca gggcgcagcc gagtcacaga caggagacga
     2161 gggtccaagt ggaagcagcc gttgaaccga aggtccgcac gtacggcaac tggcgcaagc
     2221 cgatgtcggc cggtcttggt caactcggcc tgatcggcac cggcctgatg ctggtcagcc
     2281 tgatcatcgt gatcctcacg gtggggttct tcgggctgct gaccgccgcc gtcgtcggac
     2341 tgatcctcac ggtcatgctc ggcacgctcg tgatccacga tcgtcacggc aagacggtcc
     2401 tgcagcgcat ggcggcctgg atcggctggc gtcgcacccg ctcacgcggg gcgcacctgt
     2461 accgcggggg gccgctcggt cgcaatccgt ggggaacctt ccatctgccg gggctggccg
     2521 cccgctcgac gctcagtgag gcccgcgact cctatgaccg gccgtttgcg ctgcttcaca
     2581 tgccgacgac caaccactac acggtcgtgt tcggcactga gcccgacggg gcgcagctcg
     2641 tcgaccagtc gcagatcgac gtgtgggtcg cgcactgggg cgagtggctg gcatcgctcg
     2701 ccgacgagcc cggactcgtg gccgcgtccg tcacgatcga gacatcgccc gacagtgggg
     2761 ctcggctgcg acgcgaggtc ctctcccgtc tcgatccggc ggccccggcg ctcgcccagg
     2821 agatgttgca cgaggtcgtg gagacctatc cggcgggctc ggcgaccgtg aaggcgtacg
     2881 tcgcgttgac gttcagtgca acgtcgcgcg cgggcggcaa gcgccgcgac gagcacgagg
     2941 tcacgcgaga ccttgccgcg cgcctgcccg ggctgaccgc gggcctgacg ggcaccggtg
     3001 cgggtgcggc gcgaccgctg agcgcccagc agctctgcga ggcgatacgg atcgcgtacg
     3061 agccgcgtgc ggcagcgctg atcgacgagt cctacgacgc cggcacggtg cccgagctgg
     3121 actggagcga cgtcggtccg tcggccacag aggcgcgctg ggacacctac cggcacgacg
     3181 gcgcgacatc ggtgacgtgg tcgatgaccg ccgcgccgcg cggcggtcat cgaccacgtc
     3241 accgatgtcg cgccgtcgtg ccggtaggtg tccc
//
LOCUS       DATLFO010000156         4020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_122113, whole genome shotgun sequence.
ACCESSION   DATLFO010000156 DATLFO010000000
VERSION     DATLFO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122113"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VM841_05880"
     CDS             complement(<1..281)
                     /locus_tag="VM841_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89748.1"
                     /translation="MTATATSPDLDLDALNGLLSRVTFRPHGGPHVPIRAERRPDGRV
                     FLTHDLDVPDSRCGPVPGYGFKSRGFSQYRGSTVDEASIRRELTFHVAST"
     gene            complement(278..976)
                     /locus_tag="VM841_05885"
     CDS             complement(278..976)
                     /locus_tag="VM841_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89749.1"
                     /translation="MLANLAQHAATSAASDAVATLTRNDAPIHDESIDGAPHVVIPPT
                     LVDYRQDAARAVMLSKRRAREAHGGDWISTPHDADLDAMDRALSTITFSWPGSTIAGL
                     WAERWSDIGSSDGNRKGRPGTGHLYIASESHRANLAGERRTDDDSRDRRMVDRGHPCT
                     RFYGDPYNEEQVLAGAAHHLAWFAAHEVLEYASVGDSRLYDPHLAGEPEIRLPGLVAP
                     YDEHRGPEPASDAK"
     gene            complement(1370..1636)
                     /locus_tag="VM841_05890"
     CDS             complement(1370..1636)
                     /locus_tag="VM841_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL89750.1"
                     /translation="MKAPPGDDLSDRRAERARERADAVARLREPEFGPVSVVQRVLGV
                     GRSRAYQLCASGEIASIRLGRSLRVDLASLDQYVADLRADRTLA"
     gene            1818..2369
                     /locus_tag="VM841_05895"
     CDS             1818..2369
                     /locus_tag="VM841_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89751.1"
                     /translation="MADALAGLAENEEDAEARLEAALADAVRTAAALRYDAYDLAPDV
                     LHVDRMTARRLRDLRQLAGPDETVLTQESLAEHMNALGFKWSRVTVVDIERDARRLSV
                     AELFGLALLFRVGIGELVAPGRDQRLLVADEVGPLDARAVAELVSGRGGARWAVAARV
                     LPEGVEGPAKELWARRRAAEGDK"
     gene            2370..3647
                     /locus_tag="VM841_05900"
     CDS             2370..3647
                     /locus_tag="VM841_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVL89752.1"
                     /translation="MPKKRSNGEGGIWKRKDGKWIGVLHVGWEPVRDKETGRRVLDAD
                     GAPKMRRVRRHFVGDKREEVAKRLSDAAKEFASVGSLPDNKETVESYLAWWLRDELPG
                     TVKESTAAGYSTVLHRYVTPHLGRAKLASLTPKQLRSWMRQLEADGLSPRTCKQARAI
                     FHRALRDAVDKEILTRNPMTTVRGPSLKGDKGKKDALTADQARAVIAATRQHVMPETR
                     AKQDPMAAPRFCDVRGCRNRGHALEAAFTLALHTGLRRGEFLGLRWSDVDLEAGKVSV
                     RQTVKWLPGHGVMFDTPKSEAGSRSFRISAAVVESLKAHRVKQNAERLAAKEWTETDL
                     IFSTSTGTAYDPRFVLRMYQAASERAGLGKLRLHGCRHTAATLMLNQGVPLERVGKML
                     GHADFRITVDVYGEFDEDSTDGAEAMEAALGVG"
     gene            3774..3914
                     /locus_tag="VM841_05905"
     CDS             3774..3914
                     /locus_tag="VM841_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89753.1"
                     /translation="MTNEQTTDPTTDTAWQIRYLGGIVESLQARVCELEEALAIGSSA
                     TK"
BASE COUNT          760 a         1201 c         1436 g          623 t
ORIGIN      
        1 gtcgacgcga cgtggaaagt caactcacgg cggatggaag cctcatcgac tgtcgagccg
       61 cggtactgcg agaagccgcg ggacttgaag ccgtagccgg ggaccgggcc gcatcgactg
      121 tccggcacgt cgaggtcatg ggtcaggaac acccgcccat ccggtcgacg ttcggcgcgg
      181 atcggaacgt gcgggccgcc gtgagggcgg aaggtgacgc gggatagaag cccgttgagc
      241 gcgtcgaggt cgaggtcagg ggatgtcgcc gtggccgtca cttggcatca ctggccggtt
      301 caggcccgcg gtgttcgtcg taaggagcga ccaggccagg aagtcgaatc tccggttccc
      361 ctgcgagatg cgggtcgtac aaccgactat cgccgacgga cgcgtactcc aagacttcgt
      421 gcgccgcgaa ccaggccaga tggtgagcgg cacccgcgag cacctgttcc tcgttgtacg
      481 ggtcgccgta gaagcgggtg caagggtggc cgcggtcgac cattcgccta tctcgcgagt
      541 catcgtcggt acggcgctcg cccgccaagt tcgcccggtg cgactcggac gcgatgtaga
      601 gatgcccggt tcccggtcgg cccttgcggt tgccgtcgct cgatccgatg tcagaccagc
      661 gttcggccca cagaccagcg atcgtcgatc cgggccagct aaaggtgatg gtcgacagcg
      721 cccggtccat ggcatcgagg tcggcatcat gcggggtcga gatccaatcg ccgccgtgag
      781 cttcccgcgc ccgccgcttc gacagcatga cggcgcgggc cgcgtcctgt cggtagtcaa
      841 cgagggttgg cgggatcacg acatgcggcg cgccgtcgat ggactcatcg tggatcggcg
      901 catcgttccg cgtgagggtc gctaccgcat ccgaggccgc agaggtcgcc gcgtgctggg
      961 ccaggttcgc aagcacgttc gcctcgtcgt gagggtcgcc agagaacgtg gtcgtaaggc
     1021 gaaaccccgc tgcgcgatcc gtgctgaaga gaactagccg ttgtccgcgc cggcccacat
     1081 agatcgcccg agggatgccg ttgggcgccc atcgaacatc gagcaggact gagcgaatcg
     1141 cggctagatc cgggtcacgc tccgggggtg tcatgccagc aggcgcactc atcgcactcg
     1201 ggaccgtgct cagagatgca ggcgactacg gactgtccgc gtgcgacggc atcggccagg
     1261 gcggcgcgag cggcgtacac ggcatcgagg atgggggaga cgcgcggttc ggcgacagac
     1321 cacaagtagt cgaactcggc gcgctgatcg gcggaaggcg cggcggccgt catgcgaggg
     1381 tccggtcggc gcggaggtcc gctacgtact ggtcgagcga tgccaagtcg acgcggaggg
     1441 atcggccgag acggatcgaa gcaatctcgc cgcttgcaca gagctggtag gcgcgggacc
     1501 tgccgacgcc gagaacgcgc tgaacgaccg agacagggcc gaactcgggt tcgcggagcc
     1561 gggctacggc atcggctcgc tcgcgtgcgc gttcggcgcg gcggtccgag agatcatcgc
     1621 cggggggtgc tttcacttgg aaactcctgg gttagacgcg gcggccggca gcgagctttc
     1681 ccttgacaca gcgtacacgg tgagagaagg taatgcaaac gggacgacgg attaccttac
     1741 acgtaacgcg tgtagccgct ggacagcgag gacgaatgag gcgacttccg aaggatctcc
     1801 gaccagctct cgatgcgatg gcggacgctc tcgcgggtct ggcggagaac gaggaggatg
     1861 ctgaggcacg gctagaggcc gcccttgccg acgcggtgag gaccgctgcc gctctccgtt
     1921 atgacgccta cgacctcgcc cctgacgttc tccacgtcga ccggatgacc gcacgccggc
     1981 tgcgcgacct tcgccaactt gcaggaccgg acgaaaccgt cctgacgcaa gagtcgcttg
     2041 cggaacacat gaacgcgctt ggtttcaagt ggagtcgcgt gaccgtggtc gacatcgagc
     2101 gggatgcgcg ccggctctca gtcgccgaac tgttcggact cgctctcctg ttccgtgtgg
     2161 ggatcggcga actggtcgcg cccggaaggg accaacggct cttggtcgcc gatgaggttg
     2221 gcccactcga tgccagggct gtcgctgaac tggtgtccgg gcgcggtgga gcacggtggg
     2281 ccgtagcggc gcgtgtgctg ccagagggtg ttgagggtcc agccaaagaa ctttgggcgc
     2341 ggcgtcgagc cgcagaagga gacaagtaga tgccgaagaa gcgttcaaac ggcgaaggcg
     2401 ggatctggaa gcggaaggac ggcaagtgga tcggagtcct tcatgtcgga tgggagccgg
     2461 tacgcgacaa ggaaaccggg cggcgcgtgc tcgatgccga cggagcaccg aagatgcggc
     2521 gcgttcgtcg acacttcgta ggggacaagc gcgaggaagt cgccaagcga ttgagcgacg
     2581 cggctaagga gttcgcttcc gtcgggtccc ttccggacaa caaggagaca gtcgagtcct
     2641 accttgcctg gtggctgcgc gatgagcttc ccggcaccgt gaaggagtca acggcggccg
     2701 ggtactccac ggtgctgcac cgctacgtaa ccccgcacct tggccgggcc aagctggcga
     2761 gtctcacgcc gaaacagctc agaagttgga tgcgtcaact agaggccgac ggactgtcgc
     2821 cgcggacgtg taagcaggcg cgagcaatct ttcaccgcgc gctgagagat gcggtcgaca
     2881 aagagatcct gacgagaaat ccgatgacca cggttcgcgg gccgtcgctg aaaggtgaca
     2941 aggggaagaa ggacgctctc accgccgacc aggcgcgggc cgtcatcgcc gcaacacggc
     3001 agcacgtcat gccagagacg cgggccaagc aggatccgat ggcagcgcca cggttctgcg
     3061 acgtgcgcgg atgccggaac cgggggcacg ctctggaagc cgcgttcacg ctcgcactcc
     3121 ataccgggct acggcgcggc gagttcctag gacttcgctg gtcggacgtg gatctagaag
     3181 cgggcaaggt cagcgttcga cagaccgtga agtggctacc cggccatgga gtcatgttcg
     3241 acacgccgaa atcggaggcc ggtagccggt ccttccggat ttcggcggcc gtcgtcgaat
     3301 cgttgaaggc gcatcgggtg aagcagaacg ccgaacggct cgcggcgaag gagtggaccg
     3361 agacggacct gatcttctcc acgtcgaccg gcaccgcgta tgacccgcgg ttcgtgctca
     3421 ggatgtacca ggcggccagc gagcgggccg gactcgggaa actgaggctt cacggatgca
     3481 ggcacacggc ggccacgctg atgttgaacc agggagtgcc gttggagcgg gtcggcaaga
     3541 tgctcggaca cgcggacttc cgcatcaccg tcgatgtcta cggcgagttc gatgaggact
     3601 cgaccgacgg cgcggaggct atggaggccg ctctaggagt cggatagccg cggccggatc
     3661 aaggctgcgg gctgccacga tctagccggc tgcggtcagg gttggtgaga gggacctggc
     3721 cgcagcgggc tagcaccatc ggcggaacca aacccacaac agcaagggag acaatgacca
     3781 acgaacagac caccgacccg accaccgaca cggcatggca gatccgttat ctcggcggga
     3841 tcgtggagtc actgcaagcg cgggtgtgtg agctggaaga ggcgctagca atcgggagta
     3901 gcgccacgaa gtaggcggat ccaagtcgag gccgcccggt cgaggatgat cgagcggcgt
     3961 cgacttggat ccgctttcac acgttcgacg gatgagtagg tgttcccgag atgcgtgctc
//
LOCUS       DATLFO010000157        25561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_105043, whole genome shotgun sequence.
ACCESSION   DATLFO010000157 DATLFO010000000
VERSION     DATLFO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105043"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VM841_05910"
     CDS             complement(<1..325)
                     /locus_tag="VM841_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89754.1"
                     /translation="MFPTHGGPWSLMRSRSDLRGHRLPNGLIRRALGYAAHYKAKVAA
                     FLVATVAGSFLAVIPPLLFRALLDRVLPAKDQDGLAVLAVAAVGVAIGTALLSLAARW
                     FSANVG"
     gene            complement(358..864)
                     /locus_tag="VM841_05915"
     CDS             complement(358..864)
                     /locus_tag="VM841_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89755.1"
                     /translation="MPRLAGEAPGAGPAPASGGDDVAAKPRRRSRRLAWTSLAATIVL
                     AAVLAASLSAQSRMPRGGYRTIEGLTHALAERGYRCRAPVADPAPPAGVRAAAACTIG
                     AAPVWLVTFASPERRNRYLITAAPGRPAAIAATWAVITDDASLARDIAVSFRVRFAAD
                     ARSEDAAE"
     gene            complement(1200..2276)
                     /locus_tag="VM841_05920"
     CDS             complement(1200..2276)
                     /locus_tag="VM841_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HVL89756.1"
                     /translation="MRWLREEFGEVRWVFVGVGGLLVYGITGYLLLGYHLAAALYNTV
                     LVVTTVGFTPPAEPGTAERLFTSSMAMFGVAAFLAALAMISAALLEGRIGILTRRKRM
                     DRHIASMSGHYIVSAYGRVGRAAARELEAEGIPFVVVDRLEELEDQMRSDGVVHTLGD
                     PTDENVLRAVGVERARGLISAVDSDADNVYIVLTARSLNPNLFIVARASESAAADRLY
                     RAGADRVVSPYVSSGRHMAMLALQPRVLDYLEIAGESTRLEEVLVEPASPLIGQSVAA
                     ASDGGTALVVRRADGRVEPNPRADLVLEQGDLLVLLGGQRPKAGTRTGRKPRAGAKPR
                     AAAEPRAGAKRRAKTKRRAGAEAR"
     gene            complement(2312..2394)
                     /locus_tag="VM841_05925"
     tRNA            complement(2312..2394)
                     /locus_tag="VM841_05925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2359..2361),aa:Leu,seq:caa)
     gene            complement(2447..6430)
                     /locus_tag="VM841_05930"
     CDS             complement(2447..6430)
                     /locus_tag="VM841_05930"
                     /inference="COORDINATES: protein motif:HMM:NF037006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL89757.1"
                     /translation="MAASFPPGARRRALPGMLVLALLALPPAARATPPPVIVHPGSSG
                     PWTILNRDAPTGAETSGPTPRGTRTGFADGPAATPAGLGSLQMIVGASDDSARIETGL
                     LGGLQPADIGPLSYSTYVRQGRAGAAAAMQLLIDRNGDGRFRTAEDDVLTFEPVHQHG
                     GAPGTPVPNQCGGVNGCVATGGWQAWDAAAGGWWSLRAGTYGPPLVTLAGYQALHPAA
                     RVAGDVAALRVIAGGGPSWEGFDGFVDAVRAGGGLYDFELMARVADCAAATDDIAAVQ
                     TAIDAAPPGFGVRLRGSCDFGPAAPQGGTAASIEAAAVVVGSASPARDLVIEPDGPPG
                     SAAVTGTGVQAAFFVPPGARDVTIRGLRFTGFARPVVIVNAERITIGVAGSAVPSAGG
                     NALTGGVSTGSAILAVADPGGPNGTLPVRYGVAGGRTASYPAAGARLAGLTVAGNRIA
                     YDAAGVPDAVRPMIGIDVRAVGAGRIEDVRIDGNAVWFVTNEFRSFDMNGIRVQGSAG
                     VPAESVAIAGNSIGRPEELGEPAPSTASGGRVGILVHRAAGVVVERNRVRATVSATPA
                     ADVPGGGIVVADTRDAAVRDNAVVVVAGEGTEPADLGAIGVVDDLDAAFGAAGGPPAT
                     TRVQVTGNRIGTPGQPGIGAHRGLVVAGASWVDASDNTVVFSQGPAIALGPEIRGPAG
                     SLPRSVTASVLCGNDLHGSVDDPNRIEVSGATTNANFPGGSLVPTNGECAPAGIAIAE
                     TGGSTTVAEAGGTDVYTVALTLRPRTDVTVSIAGGANATAAPSVLTFTPVDWATPRTV
                     TIEAVQDSVPEGTHLQAITHTVASADAAYAGIARTLLVFVLDDDPGSVQIAETGGSTE
                     VAEGGATDTYTVVLGSRPGADVRVIATAGSQLVVSPATTVFTPADWSVPRTVTVSAAD
                     DTLREGPHLATIAHRAVSADPNFDGTPVGGIVVRIADNDAPPPPVVTSPAAGSRIRVS
                     GVVIAGRGEPQATVTVREGGVPLGSGVVGAAGTWSIGPVVFTEGAHTVQATQTDPNGF
                     ASGPASHTFTVDLTPPQAPVIVSPAEGQVFLFAGIEIRGTAEPSAVVVVSEGAIARST
                     VSDATGAWKVVMVFAEGVRTVVATIYDGAGNAGPSSPPRTFTVNADGIPPDPPAVISP
                     PQDGVVAERFTLTGTTEPLARVEVWEGSIPILGGSAGSDGFFAVELELESRTWLLRVR
                     AIDRSFNVGAFAPVRRVHVDGVAPTVSVRKPVPLPVVPVPPDEEVTAFGAASDNYGVD
                     RVEVTYTNLFGDAITRRATLCCEEDGVLRWSDRTPLARGRYLVTAYSYDIAGNRSRGS
                     SIEILRL"
     gene            6652..7134
                     /locus_tag="VM841_05935"
     CDS             6652..7134
                     /locus_tag="VM841_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89758.1"
                     /translation="MRRGLLVATIAFVALHGVASHAGPIASCGSQAGPNGVLGNPSVS
                     GVCSAPVPVTETGEVTIRLHPGPAFTGVLTAKVTAPGYGGLAVTGIYVAGELVNGMDE
                     DTLTLVAAEDAVWTLSVSAGDSSTTSTYLFPPLPQQATIPGAAFGRFGAEVVPAASQA
                     "
     gene            7257..8780
                     /locus_tag="VM841_05940"
     CDS             7257..8780
                     /locus_tag="VM841_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89759.1"
                     /translation="MARLLALLAVLAAALAGTAVPGVTREPAPCAPPAAADPMPEGGS
                     HDHADPAHHRFQCGLRAVASLDLRDTVPDAQMFGELDIAGEILVMATTFPDAGFVLFD
                     ISDPLSPAVLSRFRGPACETKTDVDCGADVKITPDARYVFIGIQRTGGGGEAGRLQPG
                     IMTVDVSDPRAPREIAFERIPPVGVHMLAFHKIGDHGWVFARARGLGRANAQPGVAIF
                     RVEPGGKLLKTGEIVADGAHDVSLYDDPKDKTTYLYLSGAQAGVLQVYDVGDPIRPHK
                     AGEWQPAVEVPGNAWYVHNAWTFRDGSRRYTLAGPELYTEFAPLPPGHGSVAGPMWLL
                     DTTDHANIRMIGEWRNPGGHAAGDLTFSPHNSWYAGDGVSWTAHYHGGVWVLDWREVM
                     AGDARRPREIGYHVPHAVRRDFVTSDAERKVFTGENMQVRPLIWDVVATGRHGYASDI
                     NGGLVVLERPEGPAAGAQPPGDGSVPQGSGGGNAAAIGIVVLLVVGAPALAYAMRRR"
     gene            complement(8777..9637)
                     /gene="trpA"
                     /locus_tag="VM841_05945"
     CDS             complement(8777..9637)
                     /gene="trpA"
                     /locus_tag="VM841_05945"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HVL89760.1"
                     /translation="MGVFKRDERLPEHTVVTTVGDGSPRRSLSSAFETGKREGRALLM
                     PFLVCGYPDPDAFLACVEAAGTAGADVLEIGIPFSDPIMDGPLIAAASTKVLDRGQTV
                     DDAFGLLARASAAFGRPMVAMTYYNLLLRRGLKRFAGDCAAAGVSGVIVPDLAVEESL
                     QWGVACEQAGIAAVFMASVTSSPERLRQIAHTSEGFIYAASTLGVTGVRDSLGDRART
                     LVASLREATSKPVAVGIGVSTPEHAREVAGYADGVIVGSALVQAIDDGGPEPAKRVVQ
                     LVTALRDALG"
     gene            complement(9640..10839)
                     /gene="trpB"
                     /locus_tag="VM841_05950"
     CDS             complement(9640..10839)
                     /gene="trpB"
                     /locus_tag="VM841_05950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HVL89761.1"
                     /translation="MARVRTDPDSAGHYGPFGGKFVPEALMGAVGELEQAFDEARRDP
                     SFNEELDRLRRVYAGRPTPLWPARRLSERLGGPTIYLKREDLNHTGAHKINNVLGQCL
                     LAQRMGKQRVIAETGAGQHGVATATAAALFGQECVVYMGEEDTRRQSLNVFRMELLGA
                     SVVPVTAGSRTLKDAINEALRDWVTNVETTHYVIGSVVGPHPFPLLVREYQRVIGEEA
                     REQIAAAEGRLPDYVVACVGGGSNAMGIFHGFVGERDTTLVGVEAAGQGVESGKHSAT
                     LTAGEPGVLHGAMSILMQDADGQVIPTHSISAGLDYPGVGPEHAYFRDEGLATYTTAT
                     DEEALAAFRLLAHTEGIIAALESSHALAWVMREAANLDGKVVVINISGRGDKDVDTVR
                     GLLKGEA"
     gene            complement(10961..11734)
                     /gene="trpC"
                     /locus_tag="VM841_05955"
     CDS             complement(10961..11734)
                     /gene="trpC"
                     /locus_tag="VM841_05955"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HVL89762.1"
                     /translation="MSFLERITAERREDALRRRAEGALEEAKAAAEAAPPVRDFEAAL
                     RVPGLSLIAEIKRASPSKGVIAAGADEVAIARAYEDGGAAAVSVLTEPNHFRGTLEDL
                     RSVRSAVEVPVLRKDFVCDELHVYEARAAGADAVLLIVAALTQSELVALIDAAAVCGM
                     AADVEVHDPDEVGRAVDAGARIVAINARNLATLDVDLATVEKVRPLIPGGVLAVAESG
                     IETVADVRRMRDAGVDAVHIGETLMRAPDPAAAIRDLLE"
     gene            complement(11757..12722)
                     /locus_tag="VM841_05960"
     CDS             complement(11757..12722)
                     /locus_tag="VM841_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HVL89763.1"
                     /translation="MRRAAGSVVVVLLAAACTAPQAPRTVPSASPSPSASPSPSVTPS
                     VPATPAPAVFDGARALRTVQALATGVGPRDAASAAYRRAADLVERSFTAHGYSVRRQT
                     VGVPAGRSGERDVPAGETVNVIATPPGYEPARPHLLVGAHLDTVPVSPGANDNASGIG
                     VMLELARLARLAPTRMPVVWVAFGGEERRVPGDAGALFGSRAYLDSMPRAQHAALKGV
                     IVMDVVGRGTHVQIASDGKTAPPVIDALEATAMRLGIASRVRVLTGLFSDHRPFERAG
                     FPVAWLYTGEFDELHTRRDVAAIVDADALRRTGVVAWETLRTLSL"
     gene            complement(12719..14104)
                     /locus_tag="VM841_05965"
     CDS             complement(12719..14104)
                     /locus_tag="VM841_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL89764.1"
                     /translation="MRFSLRGRLAAALAAVAAVSVGITAAVAYGLLDRFAREQALQQL
                     RRDASVLSRQSLSGDDAPRPRVPVHLRTALQLLQASGDHFARIGPGQRIVAGDAVAEA
                     VAAAVVRERGTASRAEGMIEAGGTRYAYVLVPIFEGSRSPGAVLLARPASLAADLLRP
                     VAARVALGGMVALAIALAISWVIARRLSGRVHRLAEAAGSIAAGDLAHRVSVEGGDEI
                     AELAHRFNLMASSLSEARRREREFLASVSHELRTPVTAIRGYAGAIADGTASSAQEQA
                     DALGVIDLEAERLERLVQDVVDLGRLGSDQFRLELGEVDVSATIDETVLAHAAQAADA
                     GVHLDAHAAPSLRGVTDTARVRQILSNLVENALRVTPPGGRVRITGRAEDGWIVIDVA
                     DTGPGIAAADLPHVFERGYLWNAYTGRRSVGTGLGLAIVRELAPALGGRVDVASEPGR
                     GTTFRLWLPAV"
     gene            complement(14101..14781)
                     /locus_tag="VM841_05970"
     CDS             complement(14101..14781)
                     /locus_tag="VM841_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL89765.1"
                     /translation="MATGTILVVEDERSISSLVEMYLRKEGYEVVCVDDGAAAVEAAR
                     TARPALVVLDVMLPGMDGLEICRRLRARSAIPIIMLTARDTDVDRIIGLEIGADDYVT
                     KPFNPRELVARVKAVLRRSEPAPEDRLEDTFTLGPVFIDVARRLVRVGERAVELTPKE
                     FDLLAFLARNRGIVFGRDRLLERVWDYERAIDTRTVDSHVRSLRAKLGEDAQVVKTIR
                     GVGYKAET"
     gene            complement(14854..15543)
                     /locus_tag="VM841_05975"
     CDS             complement(14854..15543)
                     /locus_tag="VM841_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89766.1"
                     /translation="MRARTRMAALVAVAGLVAFSAVASGSPVAALGERADRIDRADQG
                     ERAAAAKERKRMNHGIVHSETIVQTDEGTFVTKIVDAGVVSAVDGNTVTIERADGVSV
                     TVTATDETKIRRNGEEATTADLRTGDHAMIMQVVEDGTTTVRFIRAGDEEFKAERTEN
                     REKRRAETEERRDKSRAEMEKRRAEMEERRDKRRAEMEERRAEMEKRRDEMRERPGEP
                     DPGPEEASFSG"
     gene            complement(15648..16148)
                     /locus_tag="VM841_05980"
     CDS             complement(15648..16148)
                     /locus_tag="VM841_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89767.1"
                     /translation="MISLLAALVEDRRLAVWTAGALAIVAGSAAPWVHVASPLTPLTE
                     LGLETDGKITVVCGAAALGLCIAFVRLRGRDLAVGALVAAGAAGALSLVYLLRVRAAS
                     SRVLARLLEVDGAAVSTEFAARPGLGVWVVLAGAAAVAAVTLALLAGRNRPSAGRETA
                     SKAEKR"
     gene            complement(16145..16684)
                     /locus_tag="VM841_05985"
     CDS             complement(16145..16684)
                     /locus_tag="VM841_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89768.1"
                     /translation="MSPRTRLLLGSLAGLAAAVVVLAAANATWRTVTTSDFVVTIGEQ
                     QVVLDRGRAIKGTTQDASLALFAIVGAAAALVAPMLSATPRMGASAFLGASGLMISAS
                     AAITRGIESVPEEWLSGAHADRLALSPPTAAPLIAVAGGIAMIAAAAWLLTRVRNAPR
                     LTMPEGPPGAAERDEGAWE"
     gene            complement(16681..18174)
                     /gene="trpE"
                     /locus_tag="VM841_05990"
     CDS             complement(16681..18174)
                     /gene="trpE"
                     /locus_tag="VM841_05990"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HVL89769.1"
                     /translation="MSASAYQPSREEFVRLAATHTVVPVWREVLGDLETPVGAYRKLG
                     ASPNSFLLESVEHGERWGRYSFIGVDPFLVMTSRDGVVHLEGAAPPGVSDAPDPLSAL
                     EAALSAYRAPTLSGLPPLHGGAVGYLGYDVVRYIERLPQTTDDDRGLPEMAMLFIGRL
                     LVFDHLRQRMTVISNVAIGDDPAAQYDEAVAGAEAIVERLASTPTHSTPVSPVLDAPP
                     EGVRSNMEPEDFHRMVEQAKEHIVAGDVFQVVLSQRFEMDTTADPFDVYRVLRLINPS
                     PYMFFLQYPGLSVVGSSPEPLLKVVEGEAVQRPIAGTRWRGRTEDEDAKLADELLADP
                     KERAEHVMLVDLARNDLGRVCTPGTVRVAELMTIERYSHVMHIVSEVRGALQPGTGGI
                     AALKASFPAGTVSGAPKIRAMEIIDALEPARRGPYAGAVGYLDFSGNLDTCITLRTAL
                     ITGGKAYVQAGAGIVADSDPHAEETECRNKARALLTAIRAAEGLTAP"
     gene            complement(18179..18499)
                     /gene="hisI"
                     /locus_tag="VM841_05995"
     CDS             complement(18179..18499)
                     /gene="hisI"
                     /locus_tag="VM841_05995"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HVL89770.1"
                     /translation="MMVPELTYDANGLIPAVVQQHDTGEVLMVAYMNAESLRKTLETK
                     TTWFWSRSRQEFWNKGATSGNTQAVTEVLYDCDADTLLVKVDAAGPACHTGERTCFYR
                     AIEG"
     gene            complement(18510..19283)
                     /gene="hisF"
                     /locus_tag="VM841_06000"
     CDS             complement(18510..19283)
                     /gene="hisF"
                     /locus_tag="VM841_06000"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HVL89771.1"
                     /translation="MTLARRIVPCLDVDAGRVVKGINFVDIRDAGDPVEMAAIYDREG
                     ADELVFLDITASVEQRDTMIDVVRRTAEQVFIPLTVGGGIRTPTDVRRMLRAGADKVS
                     FNTAAVRDPSVLRAAADEFGAQCIVIAIDARRRPGDQGGGWEVVVEGGRTPTGLDAVH
                     WAVEATGEHGAGEVLLTSMDGDGTKDGYDVALCAAVADSVGVPVIASGGAGTPQHFAD
                     ALTDGRCDAALAASVFHFGEMRIADVKRHCAAAGLPIRL"
     gene            complement(19280..19993)
                     /gene="hisA"
                     /locus_tag="VM841_06005"
     CDS             complement(19280..19993)
                     /gene="hisA"
                     /locus_tag="VM841_06005"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HVL89772.1"
                     /translation="MILLPAIDLRGGRCVRLTQGDFDQETVYDDDPVAVAKRFEAAGA
                     EWLHVVDLDAALEGVPRNRDAIRAVVDAVGLKVEISGGIRDEDAVRAALAAGATRIVI
                     GTRALNDPAFVTRVVAEHGDAIAIGLDVRGTRLQARGWTEDAGELYPTLAALDEAGAR
                     TYVVTDVARDGMLSGPNLDLLADVMSRTPAALVASGGVSSLADLRALARLGVPECIVG
                     KALYAGAFTLPEAIKAARA"
     gene            complement(19990..20586)
                     /gene="hisH"
                     /locus_tag="VM841_06010"
     CDS             complement(19990..20586)
                     /gene="hisH"
                     /locus_tag="VM841_06010"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HVL89773.1"
                     /translation="MTRLAVLDFGMGNLRSVAKAIEKAGATVDVVEDVPVNADGLVVP
                     GQGHFGSCVTNLGARTKDIVRWIEDGKPYLGLCLGLQVLFASSEEADAAGLGVLPGTV
                     KRFDGTLKIPHIGWNEVTPVGEQPIFEGIAPGTRFYFVHSYYPSPSEPADVAATTDYG
                     VTFACAAVRDTVVATQFHPEKSGEPGIALLRNFLGMCA"
     gene            complement(20583..21212)
                     /gene="hisB"
                     /locus_tag="VM841_06015"
     CDS             complement(20583..21212)
                     /gene="hisB"
                     /locus_tag="VM841_06015"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HVL89774.1"
                     /translation="MPAKKRSAARPAAKGAARTATVHRKTKEVDVTVKLTLDGDGTAT
                     AKTGLPFFDHMLEQLGKHAGFDFDVRAKGDLEVDAHHTVEDTGIAVGEAVAQALGDKT
                     GIGRFGNALVPLDEALIQVALDVSSRPFLVHNVDAKAKTIGAYDTRLTEEFLRAFAQS
                     GGMTIHVNLLAGKNPHHIVEAEFKALARALRDACAKTGGRGVPSTKGKL"
     gene            complement(21193..22284)
                     /gene="hisC"
                     /locus_tag="VM841_06020"
     CDS             complement(21193..22284)
                     /gene="hisC"
                     /locus_tag="VM841_06020"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HVL89775.1"
                     /translation="MSDGEGRPFELRDDLAKLPPYRAPQQDAPVRLNTNESPYPPPDK
                     VMAEVGDAIRKLDLNRYPDRDASGLRRALAARNGWPAEGVWVANGSNEIIQTLMLAFG
                     GPGRRALLFEPTYAMHSHIALVTGTEVAARRIAEPWVLDPGEVAEAVAETDPALTFVC
                     SPNNPTGNAQPVEAVEAALSAGSGLVVVDEAYGDFGGVSALGLAGKHDRVVVVRSFSK
                     SWRLAGARLGYLLSHPWLVEAIQVARLPYHLSATSQLIGEAVLRHAQSALGAVIEIVM
                     ERERLAKELARVPGVEVFPSDANFLLLRTPVEGTPLWKRLADGGVLVRDFSSVPGLER
                     CLRVTVGTADQNATFVETLRRVLHAGEEA"
     gene            complement(22281..23645)
                     /gene="hisD"
                     /locus_tag="VM841_06025"
     CDS             complement(22281..23645)
                     /gene="hisD"
                     /locus_tag="VM841_06025"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HVL89776.1"
                     /translation="MLEIVDLRGFTGDPSPRLPRATADQAAARASVRQVVSAVRERGD
                     AGLIEAEMRFGRERVDYLRVPDWRIEQALDDCPRDLRDALESAAARIRDYHARQAGEE
                     RAPFWRIGPAHAQVGEETRPIRRAGCYVPGGRAAYPSTVLMTAIPASAAGVSEIAVCV
                     PPAADGRVHAATLTACAIAGVDEVYACGGAQAVAAMAYGTESIARVDKIVGPGSIWVT
                     LAKHEVAMDVGVDSFAGPTEIAIVADGTAPPEFLAADLVAQAEHDPLATCLLVTPSEP
                     LIEAVNEILPKEVAAASRAGDIESALRTYGRAVLVDGIDQAVDVVNAFAPEHLELALA
                     DAGDWLPKITGAGAIFIGVWSPVSLGDYVAGTNHVLPTAGTARFASPLRVSDFIKSTA
                     TIRFEPAGLEWIAPWAVKIAGEEGLDAHARALNVRLEHIHTTMSQDGTAHEIPESPPR
                     EEQA"
     gene            23781..>25561
                     /locus_tag="VM841_06030"
     CDS             23781..>25561
                     /locus_tag="VM841_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89777.1"
                     /translation="MTGIRGPLGAALALVAALSAAVPGGAFGSVPRPGDHRLLVSFEP
                     GADRGAAHRMAGAARLRTLDAPGGWDLVSLPASAEAAAALAVYRRLPGVRAAEPDVPA
                     RAASAPDDPCIWTPCAGATQWNMAMIEAPLAWDLFPPATAQERLGADPVLVAVLDSRI
                     DATHPDFSNTGGFSSDATAGGQIDWSSQRSWVAAANRTGPLAWHGTFVAGILAAAGGN
                     AADIAGVAYPALLLPYEVLDGNGRASASAVAEAITAAHRAGARVINLSLGLEAPSHAV
                     RDALIAATSGPAPSLVVAAAGNNTRDEPFYPASYPEAMSVAGVDATGAPASCSNYNDN
                     VSVAAPARAVLSLSPMPGRLRTIDCGTSAAAPHVSGIAALLLARDPSLTPDQVRRIIE
                     STATDDAARPGRDHHLGHGIVNADGALRAAATARVSDVAAAVPPRGASTTRVTAIARS
                     PKGVTGAEIYVDAPGNPAARFLLRPADGAWGGTTEDLVGEVPVPEHGSSVRTLFVRAT
                     DGAWGPAARGRLAIDREPPRITNVRADTVVRAQGKRPAVTFTLSDDTSTRLAYTVGVT
                     GPDGVSTVWQSSRRESGAGPASATATAP"
BASE COUNT         3617 a         9240 c         9209 g         3495 t
ORIGIN      
        1 ccccgacgtt ggccgagaac catcgcgccg cgagcgacag cagcgccgtg ccgatcgcca
       61 cgccgaccgc ggcgaccgcg agcaccgcca ggccgtcctg atccttggcc ggcagcacgc
      121 ggtcgagcag cgcccggaac agcagcggcg ggatgacggc gaggaacgag cccgcgacgg
      181 tcgcaaccag gaacgcggcc actttcgcct tgtaatgggc cgcatatccc agcgcgcgcc
      241 gaatcagccc attggggagc ctgtggcccc gcaggtccga gcgggagcgc atcaagctcc
      301 acgggccacc gtgcgtcgga aacatcgcac ggagtatagg gcggccgtcc ggccggatta
      361 ttccgccgcg tcttcgctgc gtgcgtcggc agcgaagcga acgcggaagg agaccgcgat
      421 gtcgcgggcc agcgacgcgt cgtcggtgat gacggcccag gttgcggcga tcgcggcggg
      481 acggcccggc gcggcggtga tgaggtagcg gttgcggcgc tccggcgagg cgaaggtgac
      541 gagccacacc ggcgccgcgc cgatcgtgca cgcggcagcg gctcgcacgc ccgcgggcgg
      601 cgcgggatca gcaaccgggg cccggcatcg gtagccgcgc tcggcgagcg cgtgggtcag
      661 cccttcgatg gtgcggtagc cgccgcgggg catgcgcgac tgggcggaca ggctggccgc
      721 gagcaccgcc gcaaggacga ttgtggccgc caaggacgtc caggcgaggc gccgcgaccg
      781 ccgccgaggc tttgccgcga cgtcgtcgcc gccggatgcc ggggccggcc cggcgccggg
      841 cgcctcgccg gcgaggcggg gcatccggtt tgagcccccg cagaacgggc aagcccattg
      901 cggcaggtgc cggaaaacgg gatcgatccg agatcgcccc aggcagcggg gacagtcaac
      961 gtcgatttcg ggtgccgcca gggtcatcga tgacaggata tcgccggatc cgcccaacgc
     1021 aggcggcctc ggcgcaagga gatgacatgg cagacgacaa ggaacagatg tgctgcgacg
     1081 gcgaacgcac cgcagccgag caccgcgccg aatcgtccga cggcaagtgc tgcacggacg
     1141 agtagcgcag cgaagcgtcg gtcacgcggc cccgggcgac gtccggggcc gcggcatatc
     1201 tagcgggcct cggccccggc gcgacgcttg gttttggcgc ggcgcttggc cccggcgcga
     1261 ggctcggcag cggcgcgagg cttggccccc gcgcgcggtt tgcgtccggt ccgcgtgccc
     1321 gccttcggcc gctgtccgcc gagcaggacg agcagatcgc cttgttcgag cacgagatcg
     1381 gcgcgcggat tgggctcgac gcgcccgtcg gcgcgccgca cgaccaatgc cgttccgccg
     1441 tcggacgctg ccgccacgct ctggccgatc agcggcgagg ccggctcgac caatacctcc
     1501 tcgaggcggg tcgactcgcc cgcgatctcg agatagtcga gcacgcgcgg ctgcagcgcg
     1561 agcatcgcca tgtgccgtcc gctcgaaacg taaggcgaca cgacacggtc cgcacccgcg
     1621 cgataaaggc ggtcggcggc agccgactcg ctcgcgcgcg cgacgatgaa caggttcggg
     1681 ttcagcgacc gggccgtgag cacgatgtag acgttgtcgg cgtcggagtc cacggcgctg
     1741 atcaacccgc gtgcgcgctc cacgccgacg gcacgaagca cgttctcgtc ggtcggatcg
     1801 cccagggtgt ggaccactcc gtcggagcgc atctggtcct cgagctcttc gagccggtcc
     1861 acgacgacga acgggatgcc ttcggcctcg agctcgcgtg cggcggcgcg gccgacgcgg
     1921 ccgtaggccg acacgatgta gtggccgctc atcgaggcga tgtgtcggtc cattcgcttc
     1981 ctcctggtca ggatgccgat ccggccttcc agcagcgccg ccgagatcat cgcgagcgcc
     2041 gcgaggaacg ccgcgacgcc gaacatcgcc atgctggagg tgaacaagcg ctcggccgtt
     2101 cccggctcgg cgggcggcgt gaagccgacc gtcgtgacga ccagcaccgt gttgtagagc
     2161 gcagcggcaa ggtggtagcc cagaagcagg tagccggtga tgccgtagac gaggagcccg
     2221 ccgacaccga cgaagaccca ccggacctcg ccgaactctt cccgcagcca gcgcatgacg
     2281 ggattctacg ggcgcgccgg tcggcgcgcg gtgccggggg agggactcga accctcatgt
     2341 ccttgcggac aacggtgttt gagaccgtcg cgtctgccgt tccgccaccc cggcgcagag
     2401 cgaaagtata gaagccgcgg cgcgagcgcg gcgccgggct cgcttgctac agccgcagga
     2461 tctcgatgct cgatccgcgg ctgcggttcc cggcgatgtc gtaggagtag gcggtcacca
     2521 gatagcggcc acgcgcaagg ggcgtgcggt cgctccaccg gagcacgccg tcctcctcgc
     2581 agcacagcgt cgcccggcgc gtgatcgcgt cgccgaacag gttcgtgtag gtcacctcga
     2641 cgcgatccac gccgtagttg tccgatgccg cgccgaaggc cgtgacctcc tcgtccggcg
     2701 gcaccggcac gaccggcagc gggacgggct tgcgcaccga gacggtgggg gcgaccccgt
     2761 cgacgtggac gcgtcgcacg ggcgcgaacg ctccgacgtt gaaggagcgg tcgatcgcac
     2821 gcacgcgcag cagccaggtt cgcgactcga gctcgagctc gacggcgaag aagccgtccg
     2881 agccggcgct gccccccagg atggggatgc tgccctccca tacctcgacg cgcgcgagcg
     2941 gctcggtggt gcccgtgagg gtgaaccgct ccgctacgac gccgtcctgg ggcggcgaga
     3001 tcacggccgg cgggtccggc gggatgccgt cggcattgac ggtaaaggtg cgcggcggcg
     3061 aggacggccc ggcgttcccg gcgccgtcgt agatcgtggc gacgacggtg cgtacgcctt
     3121 cggcgaagac catgacgacc ttccaggctc cggtcgcgtc gctgaccgtc gacctggcga
     3181 tcgctccttc cgacaccacc accaccgcgg acggctcggc ggtgccgcga atctcgatcc
     3241 ccgcgaacag gaacacctgt ccttcggcgg gcgagacgat gaccggcgcc tgcggcgggg
     3301 tgaggtcgac ggtgaaggtg tgcgacgccg gcccgctcgc gaagccgttg ggatccgtct
     3361 gtgtcgcctg gacggtgtgg gccccttcgg tgaagacgac cgggccgatg ctccaggttc
     3421 ctgccgcgcc gacgacgccg ctgccgagcg gcacgccgcc ttcgcgtacg gtgaccgtcg
     3481 cctgcggctc gccgcggccg gcgatgacga ccccggagac ccggatgcgc gagccggcgg
     3541 cgggcgaggt cacgaccggc ggcgggggcg cgtcgttgtc ggcgatgcgc accacgatgc
     3601 cgccgaccgg cgtgccgtcg aagttcgggt cggcgctgac cgcgcggtgc gcgatcgtcg
     3661 ccaggtgcgg tccttcgcgg agcgtgtcgt cggcggccga gaccgtgacc gtgcggggaa
     3721 ccgaccagtc ggcgggcgtg aacaccgtcg tggcgggcga gaccaccagc tgcgagccgg
     3781 ccgtggcgat gacgcgcacg tcggcgcccg gccgggatcc gagcaccacc gtgtaggtgt
     3841 cggtggcacc gccttcggcg acctcggtcg agccgccggt ctcggcgatc tgaaccgagc
     3901 ccggatcgtc gtcgagcaca aacaccagca gcgtccgcgc gatccccgcg taggcagcgt
     3961 ccgccgacgc gacggtgtgg gtgatcgcct gcaggtgcgt gccttccggc accgaatcct
     4021 gaacggcctc aatggtgacg gtgcggggcg tggcccagtc cacgggcgtg aaggtgagca
     4081 ccgagggcgc ggccgtcgcg ttcgcgccgc cggcgatcga gacggtgacg tcggttcggg
     4141 gacgcagcgt cagcgcgacc gtgtagacgt cggtgccgcc ggcttcggca accgtggtgc
     4201 tgccgcccgt ctcggcgatc gcgatgccgg cgggcgcgca ttcgccgttg gtgggcacga
     4261 gcgagccgcc cgggaagttt gcattcgtcg tcgcgcccga gacctcgatc cggttgggat
     4321 cgtcaaccga tccgtgcagg tcgttgccgc acagcacgct ggcggtgacc gagcgcggca
     4381 gcgaaccggc cgggccgcga atctcggggc cgagcgcgat cgccggtccc tgcgaaaaca
     4441 cgaccgtgtt gtcgctcgcg tccacccacg acgcgccggc gaccacgaga ccgcggtgcg
     4501 cgccgatgcc cggctggccg ggcgtgccga tccggtttcc ggtcacctgc acgcgcgtcg
     4561 tggcgggcgg gccgcccgcg gcaccgaaag cggcgtcgag atcgtccacg acgccgatcg
     4621 ctccgaggtc cgccggctcg gtcccttccc cggccacgac caccacggcg ttgtcgcgca
     4681 cggccgcgtc ccgcgtatcg gccacgacga tcccgccgcc cggcacgtcg gccgcgggtg
     4741 tggcgctgac cgtggcgcgc acgcggtttc gctcgacgac gacgccggcc gcgcggtgaa
     4801 ccaagatccc gacgcggccg ccggaggcgg tggatggggc cggctcgccg agctcctccg
     4861 gccggccgat cgagttgccc gcgatcgcga ccgactcggc gggcacaccg gcgcttccct
     4921 gcacccggat gccgttcatg tcgaaggagc gaaactcgtt ggtcacgaac cagaccgcgt
     4981 tgccgtcgat gcgaacgtcc tcgatgcgtc ccgcgcccac ggcccgcacg tcgatcccga
     5041 tcatcggacg gacggcgtcg ggcacaccgg ccgcgtcgta ggcgatgcgg ttgccggcaa
     5101 cggtcagccc ggcgagccgc gcgccggccg ccggatacga cgcggtccgt ccgccggcga
     5161 cgccgtagcg gacgggaagg gtcccgttcg gacctccggg atcggcgacc gcaaggatcg
     5221 cgctgccggt cgagaccccg ccggtgagcg cgttgccgcc ggcagaggga acggcggagc
     5281 ccgcgactcc gatcgtgatg cgctcggcgt tgacgatcac gaccggtcgc gcgaagccgg
     5341 tgaagcgaag cccgcggatg gtcacgtcgc gcgcgccggg cggaacgaag aacgcggcct
     5401 gcacgcccgt gccggtcacg gccgcggagc cgggtggacc gtcgggctcg atgacgaggt
     5461 cgcgggcggg cgacgcgctg cccacgacca cggccgccgc ctcgatcgag gcggcggtgc
     5521 cgccctgcgg cgcggccggc ccgaagtcgc acgatccgcg gagccgcacc ccgaagccgg
     5581 gcggagcggc gtcgatcgcg gtctgcacgg cggcgatgtc gtcggtcgcg gccgcgcagt
     5641 cggccacgcg cgccatcagc tcgaagtcgt agagcccgcc gccggcgcgc acggcgtcca
     5701 cgaaaccgtc gaatccctcc caggacgggc cgccgccggc gatgacgcgc agcgcggcga
     5761 cgtcaccggc aacgcgggcc gcgggatgca gcgcctgata tccggcgagc gtgaccagcg
     5821 gcggcccgta ggtcccggcg cgcaacgacc accagccgcc ggccgccgcg tcccaagcct
     5881 gccagccccc ggtcgcgaca cagccgttga caccgccgca ctggttcggc accggcgtgc
     5941 ccggcgcgcc accgtgctga tgcaccggct cgaaggtcag cacgtcgtcc tcggccgttc
     6001 ggaagcggcc gtcgccgttg cgatcgatga gcagctgcat cgccgccgcc gcccccgcac
     6061 ggccctgccg aacgtaggtc gaatacgaaa gcggtccgat gtcggccggc tgcagcccgc
     6121 cgagcaggcc ggtctcgatg cgcgcgctgt cgtcggaggc gccgacgatc atctgcaagg
     6181 acccgagccc ggccggcgtg gcggccggac cgtcggcgaa gccggtgcgc gttccgcgcg
     6241 gcgtcgggcc ggacgtttcg gcgccggtgg gcgcgtcgcg attgaggatc gtccacggcc
     6301 cggacgagcc gggatgcacg atgaccgggg gcggcgtggc gcgcgcggcg ggcggcagcg
     6361 cgagcagcgc gagcacgagc atgccgggca gagcacggcg gcgcgcgccc ggcggaaacg
     6421 atgcggccat gcacctcggg ttcgtcacgc gggcgaagcg atcctggccg ccgcggccgc
     6481 ccgggaacgg ctctgccgca ttgccccatc ggccgggttc gcaatgattc aggtttcgct
     6541 ttgcccggcg ggcaaacggg acgttcgttc cggcggggcc gagagcggga ggaaagcccc
     6601 ggggttcgag cgaaatcccg cagcgtcgag gaacgaggat tcggaggagc catgcgcagg
     6661 ggattactcg tcgcaacgat cgcgtttgtc gcgcttcacg gggtcgcgtc gcatgccggc
     6721 ccgatcgcgt cctgcggctc gcaggccggg ccgaacggcg tgttgggcaa cccgtccgtg
     6781 agcggtgtgt gctccgcgcc ggttccggtc accgaaaccg gtgaggtcac gatccgcctg
     6841 catcccgggc cggcgttcac cggcgtgctc acggcgaagg tgaccgcgcc cgggtacggc
     6901 ggcctcgcgg tgacggggat ctacgtggcc ggcgagctcg tcaacggcat ggacgaggat
     6961 acgctcacgc tggtcgccgc cgaggatgcg gtctggacct tgagcgtctc ggccggagac
     7021 tcgtccacga cctccacgta cctgttcccg ccgttgcccc agcaggcgac gatcccggga
     7081 gcggcgttcg gccggttcgg ggccgaggtc gttccggccg cgtcccaggc ctgacccacg
     7141 tcgctccggc atcgcgacgc gggcgcccga ttcgtcccgc ccggccgcca tccgccaacg
     7201 gttcccgtgc gtaccaacgg gcaggggcac ctcgcctcga cgatgatgga aggccgatgg
     7261 cacgactcct tgcacttctt gccgtgctcg cggccgcgct ggccggcacg gccgtcccgg
     7321 gcgtgacgcg tgagccggcg ccgtgcgcgc cgcccgcggc cgccgatccg atgcccgagg
     7381 gcggcagcca cgatcacgcc gatcccgcgc accatcgctt ccagtgcggc ctgcgcgcgg
     7441 tcgcgtcgct ggaccttcgc gacacggttc cggacgcgca gatgttcggc gagctcgaca
     7501 tcgccggcga gatcctggtg atggccacga ccttccccga cgcgggcttc gtgctgttcg
     7561 acatctccga tccgctctcc ccggccgtgc tgtcccgatt ccgcggaccg gcgtgcgaga
     7621 ccaagaccga cgtggactgc ggcgccgacg tcaagatcac gccggacgca cgctacgtct
     7681 tcatcgggat ccaacgcacc ggcggcggcg gcgaagccgg gcgcctgcag ccgggcatca
     7741 tgaccgtgga cgtgtcggat ccacgcgcac cgcgcgagat cgccttcgag cggatcccgc
     7801 cggtcggcgt gcacatgctc gcgttccata agatcggcga ccacggctgg gtgttcgccc
     7861 gcgcgcgcgg cctggggcgc gcgaacgcgc agccgggcgt ggcgatcttt cgcgtcgagc
     7921 ccggcggaaa gctgctcaag accggcgaga tcgtcgccga cggcgcccac gacgtgtccc
     7981 tctacgacga cccgaaggac aagacgacct acctgtatct gtcgggggcg caggcgggcg
     8041 tgctgcaggt ctacgacgtc ggcgatccga tccgccccca caaggcgggc gagtggcagc
     8101 ccgcggttga ggttcccggc aacgcctggt acgtgcacaa cgcctggacg tttcgcgacg
     8161 gctcgcgccg ctacacgctc gccggccccg agctctacac cgagttcgct cccctgccgc
     8221 ccggccacgg cagcgtcgcc ggcccgatgt ggctgctcga cacgaccgat cacgccaaca
     8281 tccggatgat cggcgagtgg cgcaacccgg gcgggcacgc cgccggggac ctgaccttca
     8341 gcccgcacaa cagctggtac gccggcgacg gcgtgtcgtg gacggcgcac tatcacggcg
     8401 gggtgtgggt gctggactgg cgcgaggtga tggccggcga cgcacggcgg ccgcgcgaga
     8461 tcggctacca cgtgccgcat gccgtgcgcc gcgacttcgt cacctccgat gccgagcgca
     8521 aggtcttcac cggcgagaac atgcaggttc gcccgctgat ctgggacgtg gtcgccaccg
     8581 gccgccacgg ctacgccagc gacatcaacg gcgggctggt ggtgctcgag cggcccgaag
     8641 ggcctgcggc aggcgcgcaa ccgccgggcg atgggagcgt gccccaagga tcggggggcg
     8701 gcaacgccgc agcgatcggg atcgtggtgc tgctcgtggt cggcgcgccc gcgctcgctt
     8761 acgcgatgcg ccgccgttag ccgagcgcgt cgcgcagcgc ggtgaccagc tgcaccaccc
     8821 gcttggccgg ctcggggccg ccgtcgtcga tggcctgcac cagcgcgctg ccgacgatca
     8881 cgccgtcggc gtagccggcg acctcgcgtg catgctcggg cgtggagacg ccgatgccga
     8941 cggcgacggg cttgctggtc gcctcgcgca gcgaggcgac cagcgtgcgg gcgcgatcgc
     9001 cgagcgagtc gcgcacgccg gtgacgccca gcgtggacgc ggcgtagatg aagccctcgg
     9061 aggtgtgggc gatctgccgc aaacgctcgg gcgaggaggt gaccgaggcc atgaacaccg
     9121 cggcgatgcc ggcctgctcg caggccacgc cccactgaag cgactcctcg accgcgaggt
     9181 cgggcacgat cacgcccgac actcctgccg cagcgcagtc gccggcgaaa cgcttgagtc
     9241 cgcggcgcag cagcagattg tagtaggtca tcgcgaccat cgggcggccg aacgcggccg
     9301 aggcgcgcgc gagcagcccg aacgcgtcgt cgaccgtttg cccgcggtcg agcaccttcg
     9361 tcgaggccgc ggcgatcagc ggcccgtcca tgatcggatc cgagaacggg attccgatct
     9421 cgagcacgtc cgcgccggcg gttccggcgg cctccacgca cgcgagaaac gcgtcgggat
     9481 ccggatagcc gcacaccagg aagggcatca gcagcgcccg tccttcgcgc ttgccggtct
     9541 cgaacgccga cgaaagcgag cgacgcggcg agccgtcgcc cacggtggtg acgacggtgt
     9601 gctcgggcag ccgctcgtcc cgcttgaaca cgcccatcgt tatgcctcgc ctttgaggag
     9661 cccgcggacc gtgtccacgt ccttgtcgcc gcggccggag atgttgatca ccacgacctt
     9721 gccgtccagg ttcgcggctt cgcgcatcac ccacgcgagc gcgtgcgagg attcgagcgc
     9781 cgcaatgatt ccttcggtgt gggcgagcag acggaacgcc gcgagcgcct cctcgtcggt
     9841 cgcggtcgtg taggtcgcaa gcccttcgtc gcggaagtac gcatgctcgg gaccgacgcc
     9901 gggatagtcg aggcccgccg agatgctgtg ggtggggatc acctgaccgt cggcgtcctg
     9961 catcaggatg ctcatcgcgc cgtgcagcac gcccggctcg ccggcggtga gcgtggcgct
    10021 gtgcttgccg ctctcgacgc cttggccggc ggcctcgacg cccaccagcg tcgtgtcgcg
    10081 ctcgccgacg aagccgtgga agatgcccat cgcgttcgat ccgccgccca cgcatgcgac
    10141 gacgtagtcg ggcaggcggc cttcggcggc cgcgatctgc tcgcgcgcct cttcgccgat
    10201 gacccgctga tactcccgca ccagcagcgg aaacggatgc ggccccacga ccgagccgat
    10261 tacgtagtgc gtggtctcga cgttggtgac ccagtcgcgc agcgcctcgt tgatcgcgtc
    10321 cttgagcgtg cggcttccgg ccgtgaccgg cacgacggac gcgccgagca gctccatgcg
    10381 gaacacgttg agcgactggc ggcgcgtgtc ctcctcgccc atgtagacga cgcattcctg
    10441 gccgaacagc gccgccgcgg tcgcagtcgc cacgccgtgc tggccggcac cggtctcggc
    10501 gatgacgcgt tgcttgccca tgcgctgcgc gagcaggcac tggccgagca cgttgttgat
    10561 cttgtgcgca ccggtgtggt tgagatcctc gcgcttgaga tagatcgtgg gtccgccgag
    10621 ccgctcgctg agccggcgcg cgggccacag cggagtcggc cgcccggcgt agacgcgccg
    10681 cagccggtcg agctcctcat tgaacgaagg gtcgcgcctt gcttcgtcga aggcctgctc
    10741 gagctcgccg accgcgccca tgagcgcttc gggcacgaac ttgccgccga acgggccgta
    10801 gtgcccggcg gaatcgggat cagtgcggac gcgcgccatc gcgcggcgag tctatcagcg
    10861 gcagcgagcg tcgttgcgtt ccgttcgatt ccccggcgag catcggcggc ggcggtcgtg
    10921 acgagggtga gcgcgatgcg gatgcgccgc gttcgcgcgc ctactccagc agatcccgga
    10981 tcgcggccgc cggatcgggc gcgcgcatga gggtctcgcc gatgtgcacc gcgtccacgc
    11041 cggcgtcgcg catgcggcgc acgtcggcga cggtctcgat gccgctctca gcgaccgcga
    11101 gcacgccgcc cgggatgagc ggccggacct tttcgaccgt ggcgaggtcg acgtcgagcg
    11161 tcgccaggtt gcgggcgttg atcgcgacga tgcgcgcgcc ggcgtcgacc gcccggccga
    11221 cctcgtcggg atcgtggacc tcgacgtcgg cggccatacc gcacaccgcg gccgcatcga
    11281 tcaaggcgac gagctcggac tgcgtgagcg ccgccacgat cagcagcacc gcgtccgcgc
    11341 cggcggcacg cgcctcgtag acgtgaagct cgtcgcagac gaagtccttg cggagcacgg
    11401 gcacctcaac ggcgctccgg acgctgcgca gatcctcgag cgtgccgcga aaatggttcg
    11461 gctcggtgag aacgctcacc gcggccgctc cgccgtcttc gtacgcgcgg gcgatcgcga
    11521 cctcgtcggc tccggcggcg ataacaccct tggacggcga ggcgcgcttg atctcggcga
    11581 tcagcgacag gccgggcacg cggagggcgg cttcgaagtc ccgcacgggt ggcgcggcct
    11641 ccgcggccgc cttggcctcc tcgagcgcgc cttcggcgcg gcggcggagc gcgtcctcgc
    11701 gacgctcggc cgtgatgcgc tctaagaatg acatggctcc agtatagaag ccacgcttac
    11761 agcgacagcg tccgcagcgt ttcccatgcg acaaccccgg tccggcgcag cgcatcggca
    11821 tcgacgatcg cggccacgtc gcggcgggtg tgcagctcgt cgaactcgcc ggtgtagagc
    11881 cacgcgaccg gaaagccggc acgctcgaac ggccggtgat cgctgaacag gcccgtgagc
    11941 acgcggaccc gggacgcgat gcccagccgc atcgccgtgg cctcgagcgc gtcgatgacg
    12001 ggcggcgcgg tctttccgtc ggaggcgatc tgcacgtgcg tgccgcgtcc gacgacgtcc
    12061 atcacgatca cgcccttgag cgccgcgtgc tgcgcgcgcg gcatcgagtc gaggtaggcg
    12121 cgcgagccga acagcgcgcc cgcgtcgccg ggtacgcgcc gctcctcgcc gccgaaggcc
    12181 acccacacca ccggcatgcg cgtaggcgcg agccgcgcga gccgggcaag ctcgagcatc
    12241 accccgatgc cgctcgcgtt gtcgttcgcg ccgggcgaga cgggcaccgt gtccagatgc
    12301 gcgccgacga gcagatgcgg gcgcgccggc tcgtatccgg gcggcgtcgc gatcacgttg
    12361 acggtctcgc ccgcgggcac gtcgcgctcg ccactacggc cggccggcac gccgacggtc
    12421 tggcggcgca cggaatagcc gtgggcggtg aacgatcgct cgaccagatc cgcggcgcgg
    12481 cggtaggctg cgctcgccgc gtcacgcggg ccgacgccgg tcgccagcgc ctggacggtg
    12541 cgcagcgcgc gcgccccgtc gaacacggcc ggcgcaggcg tggccggcac ggagggcgtg
    12601 acggacggcg agggcgaagc ggatggcgag ggcgaagcgg acggcacggt gcgcggcgcc
    12661 tgcggcgcgg tgcaggcggc ggcgagcagc accaccacga ccgatcccgc cgcgcgcctc
    12721 atacggccgg cagccacaag cggaaggtgg tgccgcggcc gggctcgctg gcgacgtcga
    12781 cgcggccgcc cagcgccggc gcgagctcgc gcacgatcgc gagcccgagc ccggttccca
    12841 cgctccgccg gcccgtgtag gcgttccata ggtatccgcg ctcgaacacg tgcggcagat
    12901 cggcggccgc gatcccgggt ccggtgtcgg cgacgtcgat cacgatccac ccgtcctcgg
    12961 cgcggccggt gatgcgcacc cgaccgccgg gcggcgtgac gcgcagcgcg ttctcgacca
    13021 ggttcgaaag gatctgccgc acgcgggcgg tgtcggtcac gccgcgcagc gacggcgcgg
    13081 cgtgcgcgtc caggtgcacg cccgcgtcgg cggcttgcgc ggcatgtgcc agaaccgtct
    13141 cgtcgatggt ggcggacacg tcgacctcgc cgagctcgag acgaaactga tcggagccga
    13201 gccgcccaag gtccaccacg tcctgcacga gccgctcgag ccgctcggct tccaggtcga
    13261 tcacgcccag cgcgtccgcc tgctcctgcg ccgaggatgc cgtgccgtcg gcgatcgcgc
    13321 ccgcgtagcc ccggatcgcg gtcaccgggg tgcgaagctc gtgcgagacg ctggcgagaa
    13381 actcgcgctc gcgacgccgg gcctccgaca gggaggacgc catcaggttg aaccggtgcg
    13441 cgagctcggc gatctcgtcg ccgccttcga cggaaacgcg gtgcgcgagg tcgccggcgg
    13501 cgatcgagcc ggccgcctcg gcgagacggt ggacgcgtcc cgagagccgc cgcgcgatca
    13561 cccacgagat ggcgagcgcg atcgcgagcg cgaccatgcc gccgagggcc acgcgcgccg
    13621 cgaccggccg gagcaggtcc gccgcgagcg acgcgggacg ggccagcaag accgcgcccg
    13681 gcgagcgcga accttcgaag atcggcacga gaacgtacgc gtatcgcgtt ccgccggcct
    13741 cgatcatgcc ttccgcacgc gatgcggtgc cgcgctcgcg cacgaccgcc gccgccaccg
    13801 cctcggcgac ggcatcgccg gcgacgatgc gctgcccggg gccgatgcgc gcgaaatgat
    13861 cgcccgacgc ctgcagcagc tgaagcgcgg tccgaaggtg caccggaacc cgcgggcgag
    13921 gcgcgtcgtc gcccgatagc gactgccgcg acagcacgga cgcatcccgg cgaagctgtt
    13981 gcagcgcctg ctcccgtgcg aaccggtcca ataagccgta ggcgacggcg gcggtgatcc
    14041 cgaccgagac cgccgcgacc gcggcgagcg ccgcagcgag ccggccccgc agggagaacc
    14101 tcatgtctcg gccttgtatc cgacgccgcg gatcgtcttg acgacctggg cgtcttcgcc
    14161 gagcttggcg cgcagcgagc gaacgtgcga gtcgacggtg cgcgtgtcga tcgcacgctc
    14221 gtaatcccag acgcgctcga gcagccggtc gcggccgaag acgatgccgc ggttgcgggc
    14281 gaggaacgcg agcagatcga actccttcgg cgtgagctcc accgcacgct cgcccacgcg
    14341 cacgagccgg cgtgcgacgt cgatgaagac cgggcccagc gtgaacgtgt cctcgagccg
    14401 gtcctcgggc gccggctcgg agcgccgcag caccgctttc acgcgcgcaa ccagctcgcg
    14461 ggggttgaat ggcttggtga cgtagtcgtc ggcgccgatc tcgagcccga tgattcggtc
    14521 gacgtcggtg tcgcgcgcgg tgagcatgat gatcggaatg gcgctgcgcg cgcgcagacg
    14581 gcggcagatc tcgagcccgt ccatgccggg aagcatcacg tcgagcacga cgagcgcggg
    14641 ccgggccgtt cgcgccgcct cgaccgccgc ggcgccgtcg tcaacgcaca cgacctcgta
    14701 gccttccttg cgcagataca tctcgacgag cgatgagatc gaccgctcgt cctcgacgac
    14761 cagaatcgtg cccgtcgcca tgatcgtttg catcgtagcc gtcacagacg tgcggagccc
    14821 cgaaggaccc cccgcacgac ggctcggaag cggctatccc gagaacgagg cttcttccgg
    14881 cccgggatcc ggctcgccgg gccgttcgcg catctcgtcg cggcgcttct ccatctcggc
    14941 acggcgctcc tccatctcgg cgcgacgctt gtcgcggcgc tcctccatct cggcgcggcg
    15001 cttctccatc tcggcccggc tcttgtcgcg gcgctcctcc gtctcggcgc ggcgcttctc
    15061 gcggttctcg gtgcgctcgg ccttgaactc ctcgtcgccg gcgcggatga accgcacggt
    15121 ggtggtgccg tcctcgacga cctgcatgat catggcgtga tcgccggtgc gcaggtcggc
    15181 cgtcgtggcc tcttcgccgt tgcgacgaat cttggtctcg tcggtcgcgg tgaccgtcac
    15241 ggacacgccg tcggcccgct cgatcgtgac ggtgttgccg tcaacggcgg agacgacgcc
    15301 ggcatcgacg atcttggtga cgaaggttcc ctcatccgtc tgaacgatgg tctcggaatg
    15361 cacgatcccg tggttcatgc gcttgcgttc cttcgcggcg gcggctcgct cgccctggtc
    15421 cgcccgatcg atccggtcgg cgcgctcgcc gagcgcggcg accgggctgc ccgaggcgac
    15481 cgcggagaac gccaccagcc cggcgaccgc caccagggcc gccattcgag tccttgctcg
    15541 catcgatccc tctccttgtc ctcgagatcc ggtcgcccga ttcagcgtca accatgtcag
    15601 acggacctga tgcgaatgtg aaggccgtgt gaaaagtttc ccgccgctta ccgcttttcg
    15661 gctttcgagg cggtttcgcg gcccgcggac ggccggttgc ggcccgcgag cagggcgagc
    15721 gtgacggcag cgaccgccgc cgcgccggca agcacgaccc acaccccgag ccccggccgg
    15781 gccgcgaact cggtgctgac cgctgccccg tcgacctcga gcagacgggc caggacgcgc
    15841 gaggaggccg cgcgcacgcg gagcaggtag accagcgaca gcgcgccggc cgctccggcg
    15901 gcgacgagcg cgcccaccgc gaggtctcgc ccgcggagcc gcacgaacgc gatgcagagc
    15961 ccgagcgcgg ccgccccgca cacgacggtg atcttgccgt cggtctccag gccgagctcg
    16021 gtcagcgggg tgagcggcga cgcgacgtgc acccacggcg cggcggagcc ggcgacgatc
    16081 gcgagcgcgc cggcggtcca cacggccagg cgccggtcct cgacgagcgc ggccagcagg
    16141 gaaatcattc ccacgctccc tcgtcgcgtt ccgcggcgcc gggcggtccc tccggcatgg
    16201 tcaggcgagg cgcgttgcgt acccgggtca gcagccacgc cgcagcggcg atcattgcga
    16261 tcccgcccgc gacggcgatc agcggcgcgg ccgtcggtgg cgagagggcc agccggtccg
    16321 catgcgctcc gctgagccat tcctccggca cggactcgat gccgcgcgtg atcgcggcac
    16381 tcgccgagat catgagcccg gaggcgccga ggaacgccga ggcacccatc cgcggcgtgg
    16441 ccgacagcat cggcgcgacg agggcggcgg cggcgccgac gatcgcgaac agcgccaagg
    16501 aggcgtcctg cgtggtgcct ttgatcgcgc gtccgcgatc gagcacgacc tgttgctcgc
    16561 cgatggtcac gacgaagtcg ctggtggtga ccgtgcgcca ggtcgcgttc gcagcggcga
    16621 gcacgacgac ggcggcggca agtccggcga gcgatcccag cagcagccgc gtgcgcgggc
    16681 tcacggggcg gtgagcccct cggccgcgcg gatcgcggtc agcaaggcgc gcgccttgtt
    16741 gcggcattcg gtctcttcgg cgtgcggatc cgagtcggcg acgatgcccg cgcccgcctg
    16801 cacataggct ttgccgccgg tgatcagcgc ggtgcgcagc gtgatacatg tgtcgaggtt
    16861 gccgctgaag tcgagatagc cgacggcgcc cgcgtagggg ccgcgccgcg ccggctcgag
    16921 cgcgtcgatg atctccatcg cgcggatctt cggcgcgccc gacacggtcc cggccgggaa
    16981 cgaggccttc agcgccgcga tcccgccggt gcccggctgc agcgcgccgc gcacctcgct
    17041 gacgatgtgc atcacgtggc tgtatcgctc gatcgtcatc agctcggcga cgcgcacggt
    17101 gcccggggtg caaacccggc cgaggtcgtt gcgcgccaga tccaccagca tgacgtgctc
    17161 ggcgcgttcc ttcggatcgg cgagcagctc gtcggcgagc ttggcatcct cgtcttcggt
    17221 gcggccgcgc cagcgcgttc ctgcgatggg gcgctggacc gcttcgcctt cgacgacctt
    17281 gagcaacggc tcgggtgacg agcccaccac cgacaggccc ggatactgaa ggaagaacat
    17341 gtagggactc gggttgatca gccgcaggac ccgatacacg tcgaacgggt ccgcggtcgt
    17401 gtccatctcg aagcgctgcg agagcaccac ctggaagacg tcgcccgcga cgatgtgctc
    17461 cttggcctgc tcgaccatcc ggtgaaagtc ctcgggctcc atgttcgagc gcaccccttc
    17521 gggcggcgcg tcgagcaccg gcgagaccgg cgtggagtgc gtcggggtcg aggcgagccg
    17581 ctcgacgatg gcctcggcgc ccgcgacggc ctcgtcgtac tgggccgccg gatcgtctcc
    17641 gatcgcgacg ttgctgatca cggtcatgcg ctgacgcaga tggtcgaaca cgagcagccg
    17701 accgatgaac agcatcgcca tctcgggcag gccgcggtcg tcgtcggttg tctgcggaag
    17761 ccgctcgatg tagcgcacca cgtcgtagcc gaggtagccg actgcgccgc cgtgcagcgg
    17821 cggcaggccg gacagcgtcg gcgcgcgata ggcgctcagc gcggcctcga gcgccgacag
    17881 cgggtcgggc gcgtcggaca cgcccggcgg cgccgcgcct tccagatgca cgacaccgtc
    17941 gcggctggtc atcaccagga acggatcgac accgatgaac gagtaacgtc cccagcgctc
    18001 gccgtgctcg acagactcga gcaggaacga gttcggcgac gcgccgagct tgcgataggc
    18061 gccgacgggc gtctccagat cgccgagcac ctcgcgccat accggcacga cggtgtgggt
    18121 ggcggcgagc cgcacgaact cctcgcggct cggctggtac gcgctggcgc tcacgccgtc
    18181 agccttcgat cgcgcgatag aagcaggttc gctcgccggt atggcaggcc ggcccggccg
    18241 cgtccacctt gaccagcagc gtgtcggcat cgcagtcgta gagaacctcg gtgacggcct
    18301 gggtgttgcc cgaggtcgcg cccttgttcc agaactcctg tcgcgagcgg ctccaaaacc
    18361 aggtcgtctt ggtctcgagc gtcttgcgca gcgattcggc gttcatgtag gcgaccatga
    18421 gaacctcgcc ggtgtcgtgc tgctgcacga cggccggaat gaggccgttc gcgtcgtacg
    18481 tgagctcggg aaccatcatg tcctccatgc tacaggcgta tcggcaggcc ggcggccgcg
    18541 caatgccgtt tgacgtcggc gatgcgcatc tcgccgaagt ggaacacgct cgcggccagc
    18601 gcggcgtcgc aacggccgtc ggtgagcgcg tccgcgaaat gctgcggcgt gccggcgccg
    18661 ccgctcgcga tcaccggcac gccgaccgag tcggcgaccg cggcgcacaa tgcgacgtcg
    18721 tagccgtcct tggtcccgtc gccgtccatg ctggtgagca gcacctcgcc cgcgccgtgc
    18781 tcgccggtcg cctcgacggc ccagtgaacc gcgtccaggc ccgtaggcgt acgcccgccc
    18841 tcgaccacaa cctcccagcc tccgccctga tcgccggggc ggcggcgcgc gtcgatcgcg
    18901 atcacgatgc actgtgcacc gaactcgtcg gcggcggcgc gcagcacgct cggatcgcgc
    18961 accgcggcag tgttgaagga gaccttgtcc gccccggcgc gcagcatgcg gcgcacgtcc
    19021 gtgggcgtgc ggatcccgcc gccgacggtc agcgggatga acacctgctc ggcggtgcgc
    19081 cggaccacgt cgatcatggt gtcgcgctgc tcgacgctgg cggtgatgtc gaggaagacc
    19141 agctcgtcgg cgccttcgcg gtcgtagatc gcggccatct cgaccgggtc gccggcgtcg
    19201 cggatgtcca cgaagttgat gcccttcacg acgcggcccg cgtccacgtc aaggcacggg
    19261 acgattcgcc gcgccagcgt cacgcgcgtg ccgccttgat cgcctcgggc agggtgaacg
    19321 cgcccgcgta caaggccttg ccgacgatgc attcgggcac gccgagccgc gcgagcgcgc
    19381 gcaggtcggc gagcgagctc acgccgccac tcgcgacgag ggcggcgggc gtgcggctca
    19441 tcacgtcggc gagcaggtcc aggttcggcc ccgacaacat cccgtcccgt gcgacgtcgg
    19501 tgacgacgta ggtgcgcgcg ccggcctcgt cgagcgcggc gagcgtcgga tacagctcgc
    19561 ccgcgtcctc ggtccagccg cgcgcctgca gccgcgtgcc gcgcacgtcg agcccgatcg
    19621 cgatcgcgtc gccgtgctcg gccacgacgc gcgtcacgaa agcgggatcg ttgagcgcgc
    19681 gcgtgccgat cacgatccgg gtcgccccgg ccgccagcgc ggcgcgcacg gcgtcctcgt
    19741 cgcggatgcc cccgctgatc tcgaccttca ggccgacggc atcgacgacc gcgcggatcg
    19801 cgtcgcggtt gcgcggcacg ccttccagcg ccgcgtccag atccaccacg tgcagccatt
    19861 cggcaccggc ggcttcgaag cgtttcgcga ccgcgaccgg gtcgtcgtcg tagacggtct
    19921 cttgatcgaa gtcgccttgc gtcaggcgaa cgcaccggcc gccgcgaagg tcgatcgccg
    19981 gcagcaggat catgcgcaca tcccgaggaa gttgcgcagc agcgcaatgc cgggttcgcc
    20041 ggacttctcc ggatgaaatt gcgtggcgac gaccgtgtcg cgcacggccg cgcaggcgaa
    20101 ggtcacgccg tagtcggtgg tcgcggcgac gtcggcgggc tcggacggcg agggatagta
    20161 ggagtgcacg aagtagaagc gggtgcccgg cgcgatgcct tcgaagatcg gctgctcccc
    20221 caccggggtg acctcgttcc agccgatgtg cgggatcttc aaggtgccgt cgaaccgctt
    20281 gaccgttccc ggcagaacgc cgagaccggc cgcgtctgcc tcctcgctgg aggcaaacag
    20341 cacttgcagc cccaagcaca gacccaagta cggcttgccg tcctcgatcc agcgcacgat
    20401 atccttggtg cgcgcgccga ggttcgtcac gcacgatccg aagtgccctt ggccgggcac
    20461 gaccaacccg tccgcgttca cgggtacgtc ctcgaccacg tccacggtcg cgccggcctt
    20521 ctcgatcgcc ttcgcgaccg agcgaaggtt gcccatcccg aagtccagga cggcgagccg
    20581 cgtcacagct tccctttcgt ggacggcacg ccgcggccgc cggtcttggc gcacgcgtcg
    20641 cgcagcgcgc gcgcgagcgc cttgaactcg gcctcgacga tgtggtgcgg gttcttgccc
    20701 gcgagcaggt tcacgtggat ggtcatgccg ccggactgcg cgaacgcgcg caggaactct
    20761 tcggtcaggc gcgtgtcgta ggcgccgatc gtcttggcct tcgcatccac gttgtggacc
    20821 aggaagggcc gcgacgacac gtccagcgcg acctgaatca gcgcttcgtc gagcggcacg
    20881 agcgcgttgc cgaaccggcc gatgccggtc ttgtctccca gcgcctgcgc gaccgcctcg
    20941 ccgaccgcga tcccggtgtc ctcgacggtg tgatgcgcgt ccacctcgag gtcgcccttg
    21001 gcgcgaacgt cgaagtcgaa gccggcgtgc ttgccgagct gctcgagcat gtggtcgaag
    21061 aacggcaggc cggtcttggc cgtcgcggtg ccgtcgccgt cgagcgtgag cttcaccgtc
    21121 acgtccacct ctttggtctt gcggtggacg gtcgcggtgc gcgcggcgcc cttggcggcg
    21181 ggacgggcgg cgctacgctt cttcgccggc atgcagcact ctcctcaagg tctcgacgaa
    21241 cgttgcgttt tggtcggcgg tcccgacggt gacgcgcagg cagcgctcca ggccgggcac
    21301 cgacgagaag tcgcgtacca gcacgccgcc gtcggcgagc cgcttccaca gcggcgtgcc
    21361 ttcgaccggc gttcgcagca gcaagaagtt cgcgtcgctg ggaaagacct cgacgccggg
    21421 cacgcgcgcg agctccttgg cgagccgctc gcgctccatc acgatctcaa tgaccgcgcc
    21481 gagggcgctc tgcgcgtggc gcagcaccgc ctcgccgatc agctgcgagg tggccgacag
    21541 gtgatacggc agtcgcgcga cctggatcgc ctcgaccagc cacgggtgcg acagcagata
    21601 gcccagccgc gccccggcca gacgccagga cttcgaaaac gaccggacga ccacgacgcg
    21661 gtcgtgcttg cccgcgagcc cgagcgccga gacaccgccg aagtcgccgt aggcctcgtc
    21721 caccacgacc agccccgacc cggccgacag cgccgcctcg accgcctcga ccggctgcgc
    21781 gttgccggtc gggttgttcg gcgagcacac gaaggtgagc gcgggatcgg tctcggcgac
    21841 ggcttcggcg acctcgccgg gatcgagcac ccacggctcg gcgatgcggc gcgccgcgac
    21901 ctcggtgccg gtgacgagcg cgatgtgcga gtgcatcgcg taggtcggct cgaacagcag
    21961 cgcgcggcgt cccggcccgc cgaaggcgag catcagcgtc tggatgatct cgttggatcc
    22021 gttggcgacc cagacgcctt cggcgggcca tccgttgcgc gccgcgagcg cgcgccgcag
    22081 gccgctcgcg tcgcggtccg ggtaccggtt caggtcgagc ttgcggatcg cgtcgccgac
    22141 ctcggccatg accttgtcgg gcggcgggta gggcgactcg ttggtgttga gccggaccgg
    22201 cgcgtcctgc tgcggcgcgc ggtacggtgg cagcttcgca aggtcgtcgc gaagctcgaa
    22261 cggccggcct tcgccgtcgc tcacgcctgc tcctcgcgcg gcggcgattc gggaatctcg
    22321 tgcgccgtgc cgtcctggct catggtggtg tggatgtgct cgagccgaac gttcagggcg
    22381 cgcgcgtgcg cgtcgagccc ttcctcgccg gcgatcttga ccgcccacgg cgcgatccac
    22441 tcgagccccg cgggctcgaa ccggatcgtg gcggtggact tgatgaagtc cgacacgcga
    22501 agcggcgagg cgaaacgcgc ggtcccggcg gtcggcagca cgtggttggt gcccgcgacg
    22561 tagtcgccga gcgagaccgg cgaccagacc ccgatgaaga tcgcgccggc gccggtgatc
    22621 ttgggcagcc agtcgcccgc gtcggcgagc gccagctcga gatgctcggg ggcgaacgcg
    22681 ttgacgacgt ccaccgcctg atcgatcccg tcgacgagca ccgctcgtcc gtaggtgcgc
    22741 agcgcggact cgatgtcacc cgcgcgcgat gcggcggcga cctccttcgg caggatctcg
    22801 ttgacggcct cgatgagcgg ctcgctgggc gtgaccagca ggcaggtggc gagcgggtcg
    22861 tgctcggcct gggcgaccag gtccgcggcg aggaactcgg gcggcgcggt cccgtcggca
    22921 acgatggcga tctcggtcgg accggcgaag gagtccacac ccacgtccat ggcgacctcg
    22981 tgcttggcga gcgtcaccca gatcgatccg ggaccaacga tcttgtcaac gcgagcgatc
    23041 gactcggtgc cgtacgccat cgcggcgacg gcttgcgcgc cgccgcacgc atagacctca
    23101 tccacgccgg cgatcgcgca tgcggtgaga gtcgcggcgt gcacgcggcc gtcggcggcg
    23161 ggcggcacgc agaccgcgat ctcggacacg cccgcggcgc ttgcgggaat cgcggtcatc
    23221 aagacggtgg acggataggc ggcgcggccg cccggcacgt agcagccggc gcgccggatc
    23281 ggccgggtct cctcgccgac ctgcgcgtgc gcgggaccga tccgccagaa cggcgcgcgt
    23341 tcctccccgg cttggcgcgc gtggtagtcg cggatgcggg cggcggccga ctccaaagcg
    23401 tcgcgcaggt cgcgcgggca gtcgtcgagc gcctgctcga tccgccagtc cggcacccgc
    23461 aggtagtcca cgcgctcgcg cccgaagcgc atttcggcct cgatcagccc ggcgtcgccg
    23521 cgctcgcgca cggcgctcac gacctggcgg accgatgcgc gcgccgcggc ctgatcggca
    23581 gtggcgcgtg gcagtcgtgg cgatggatcg ccggtgaacc ctcggagatc aacgatttcg
    23641 agcataggca cgggattcta gccgagcacg cccggcggct ccggagggat ccgagcgggg
    23701 ggacgggacg gggcgatccg gcaaagagcg tccgggcaca cgcatccggc ggcgcgcctc
    23761 gcgcgttgcc cggacaggac atgaccggta ttcgcggccc gctcggtgcg gcgctggcgc
    23821 tcgtcgccgc gctgtcggcc gccgtgcccg gcggcgcgtt cggcagcgtg ccgcgcccgg
    23881 gcgatcaccg gctgctggtc tcgttcgagc ccggcgccga ccgcggcgcc gctcaccgca
    23941 tggccggcgc ggccaggctt cgcaccctcg acgcgcccgg cggctgggat ctggtgtcgc
    24001 tgccggcctc ggccgaggcg gccgcggccc tggccgtgta ccgccgcctt cccggcgtgc
    24061 gggcggccga gcccgacgtc cccgctcgcg ccgcgagcgc ccccgacgat ccgtgcatct
    24121 ggacgccgtg cgccggagcc acgcagtgga acatggcgat gatcgaggcg ccgctcgcgt
    24181 gggatctgtt tccgcccgcg accgcgcagg agcggctcgg cgccgacccg gtgctcgtgg
    24241 ccgtgctcga ctcacgcatc gacgcgactc acccggactt ctccaacacc ggcggcttct
    24301 cgagcgacgc gaccgcaggc ggacagatcg actggtcgtc gcagcgctcg tgggtggcgg
    24361 ccgcgaaccg gaccggaccg ctcgcctggc acgggacctt cgtggcgggc atcctggccg
    24421 ccgcgggcgg caacgcggcc gatatcgccg gcgtcgccta tcccgcgctt ctgcttccct
    24481 acgaggtgct cgacggcaac ggacgcgcaa gcgcgtcggc ggtcgccgag gcgatcaccg
    24541 cggcgcatcg cgccggcgcg cgcgtgatca acctctcgct cggcctcgag gctccctccc
    24601 acgcggtgcg ggacgcgttg atcgccgcga ccagcggacc ggcaccgtcg ttggtggtcg
    24661 ccgcggccgg caacaacacg cgggacgagc ccttctatcc ggcgtcctat cccgaggcga
    24721 tgagcgtcgc gggcgtggac gcgaccggcg cgcccgcctc gtgctcgaac tacaacgaca
    24781 acgtgtcggt cgctgcaccc gcccgcgcgg tgctgtcgct gtcgccgatg ccgggccggc
    24841 tccgcacgat cgactgcggc acctcggcgg ccgcgccgca cgtgtcgggc atcgcggcgc
    24901 tgctgctcgc gcgcgacccg tcgctcacgc cggatcaggt tcgccgcatc atcgaatcca
    24961 ccgcgaccga cgacgcggcg cgtccgggac gggaccatca tctcggccac gggatcgtca
    25021 acgcggacgg cgcgctccgc gccgccgcca cggcccgcgt ctccgacgtc gcggcggccg
    25081 ttccaccccg cggcgcatcg accacacgcg tgaccgcgat cgcgcgctcg ccgaagggcg
    25141 tcaccggcgc cgagatctac gtcgacgcac ccggcaaccc cgccgcccgc ttcctgttgc
    25201 gaccggccga cggcgcgtgg ggcggcacga ccgaagatct cgtcggcgag gttccggtcc
    25261 ccgagcacgg ctcctcggtc cgcacgctct tcgtgcgcgc caccgacggc gcctggggcc
    25321 ccgccgcccg cggccggctc gcgatcgacc gcgagccgcc gcgcatcacg aacgtgcgcg
    25381 ccgacacggt cgtgcgcgcg caaggaaagc gtcccgccgt gaccttcacg ctttccgacg
    25441 acacctcgac acggcttgcc tacacggtcg gcgtgaccgg cccggacggc gtcagcacag
    25501 tctggcagtc gagccggcgc gagagcggag ccggcccggc atcggcgaca gcgacagcac
    25561 c
//
LOCUS       DATLFO010000158        14144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_141514, whole genome shotgun sequence.
ACCESSION   DATLFO010000158 DATLFO010000000
VERSION     DATLFO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141514"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VM841_06035"
     CDS             complement(<1..482)
                     /locus_tag="VM841_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HVL89778.1"
                     /translation="MSTSFELDPVSRITAGAIGEPGNRTFYIQARKDEVLVTLLAEKQ
                     QVQVLATTLSQLLETLDPIEDEGLPPTSDEMDLEDPLLPEWRIGPMAIEVEEDTGLMV
                     FVAEEAGSGDEDDETAQDLAHARFVATRAQMKALAEHAEIVCSGGRPQCQFCGFPIDP
                     G"
     gene            complement(479..1093)
                     /locus_tag="VM841_06040"
     CDS             complement(479..1093)
                     /locus_tag="VM841_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVL89779.1"
                     /translation="MLLFLIRHAMTPLTGVKLAGWSSVPLSDEGVLQAKAMAERFTSI
                     PLDALYSSPVERAVQTAKPLAAVKKMRLKIRPGFGEVRYGDWEGRPLKQLAKTQHWRD
                     VIAHPSEGRFPGGEALRETSARVVAAAAEVAAGHPKGMAAIVTHADVIKMLLAHYAGI
                     HLDLYARLAVGPASVSAVWVGDGAPRILKVNDNGSLDELVPRKK"
     gene            complement(1132..2292)
                     /locus_tag="VM841_06045"
     CDS             complement(1132..2292)
                     /locus_tag="VM841_06045"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HVL89780.1"
                     /translation="MTGRLVVATGLSTDPDGVAAAAEAAATAARGLSGAPCDLAAIFV
                     SAAHADAAVEIAAAVHVALEPGAMIGATGGTVAEGGREIEDRPAVAVWAAHLPDTEVE
                     PFSLEFTETPDGPTFLGWPSPPAGAAVIMLADPFTVPVDRLLDTVNTEQPGLTVVGGL
                     ASGAGAPGGHRIWQGSEVANGGAAGVVIRGRVKVRTLVSQGCRPIGTPATVTNAQRNV
                     IVELAGLPPVERLRDLWIEASPDERALIQRGLHIGRVVDEYKTEFERGDFLIRNVMGA
                     DETTGAMAVGDLVGVGETVQFHVRDAATADEDLRAAAAAVPPPAGAMLFTCNGRGSHL
                     FGAPDHDAGVLSSVWRAPLAGMSCAGEIGPVGGRVFLHGFTASLALFYDEQA"
     gene            complement(2289..2975)
                     /locus_tag="VM841_06050"
     CDS             complement(2289..2975)
                     /locus_tag="VM841_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89781.1"
                     /translation="MRAQVAAPALVLALGLAACGGDASTNDPLSGAQLVVDVKGDHAK
                     ALNADGVEPKNPPDAADVTGVRLDASEDSLSIRIAIAGSPYESASITDPLQGPAWFMQ
                     LFADTKAASPAYFVAIVREGEPSRAGEQIKGWRLSVCPGTEVCNEPAEGATLKITESE
                     VRADIPLTLLSKLKNPFTWAMQSYWNETQDPVQAHSDWVPEAARPSPGATTYSEPDTR
                     ATFPPEGSKK"
     gene            complement(2960..3790)
                     /locus_tag="VM841_06055"
     CDS             complement(2960..3790)
                     /locus_tag="VM841_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HVL89782.1"
                     /translation="MLISAKLAPTFDYATLAEFWTAADEAGYHAVWNYDHFYGLGTQS
                     QEHEAPTLEGWTTLAAMAVLVKRARVGCMVTGVTYRHPAVLANMAITVDHISGGRLEL
                     GIGAAWHEAEHTGLGIEFPPPGERVARLDEALHVIKALMTQDRATFDGRYFQLQDAIT
                     QPKAVQQPRPPIVVGGVKPKMLKVVAEHADEWNVPGADPELWSQLSKQIDDNCAAIGR
                     DPKHVRRGVQLFVHPAIDGQPEEQLAKIPEYEDRGCEHVVLSFYQPPSRDLVETCAPK
                     "
     gene            3862..4701
                     /locus_tag="VM841_06060"
     CDS             3862..4701
                     /locus_tag="VM841_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVL89783.1"
                     /translation="MGILRAILPLALALSVSAPAAATDSQIPVTVYAHRGGAAIGPEN
                     TMAAFEQAGALFAAAGVDGWLELDTQASADGTLVVIHDHTLDRTTDCAGTVIDWTYDA
                     SCNAAKRWPSFGHQPVPKLADLLARGRQATPAWGLMIEIKNIPYETNFDPAGMLVADS
                     LVAMIDASGYPKDRIIVQSFWPPSLDRVELLDPELDTMLLTTSQLGFMVWQNALYATA
                     RGYEISAPEWQSADFRAETVAAAHALGRRVITWTVDSRAAGDALIAMGVDGIITNDPR
                     IFL"
     gene            4698..5903
                     /locus_tag="VM841_06065"
     CDS             4698..5903
                     /locus_tag="VM841_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HVL89784.1"
                     /translation="MRRLLILLSALAIAGVFVPATAADPASGTLSDPSATVAWTGTHW
                     RTDDAPIPDQFDLTIDLPAGYWDANDGGVEVAIRWVSEYNVFDLYVLDAGGATVASST
                     GFPTSAHSLIMREPANGLYHVVVVPAGVQVDEERPYEGIAQVELDTTRTGPLVPDLVA
                     LPPAEFKIAAGTLSFMPVEYQETGGCYPDEMAENPSLTRCLRFDAGVANLGHGPFELR
                     LDTTTMAGEPVVWQDIYAADGSRTTIGAGTYTWHATHAHVHYQNFVEYRLHRVGEDGT
                     PGEEVVGSRKADFCMIDTRLIWFGEYGNGPRNHHFPQCNIPGGDGAGPTEMRQGIDVG
                     WADIYTWDLPGQYIDISNVPDGIYAVVLEADPSGVLSQLTRTNDISWTRIRIAGLSVT
                     ECTAPPPGC"
     gene            6174..6374
                     /gene="infA"
                     /locus_tag="VM841_06070"
     CDS             6174..6374
                     /gene="infA"
                     /locus_tag="VM841_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVL89785.1"
                     /translation="MEMEGIIVEALPNVMFRVELENGHIVLGHISGKMRKHYIRILPG
                     DRVKLELSPYDLNRGRITYRYR"
     gene            complement(6379..6726)
                     /locus_tag="VM841_06075"
     CDS             complement(6379..6726)
                     /locus_tag="VM841_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89786.1"
                     /translation="MRWGIGVFGRALLVILAVLALSLPAFAGESCVSEPDSNPGSVPG
                     VTLGPATKCVRDDGGTTTADQAPAGARGEGQPISWVTIAGAGLAVGGFSYGSWRVRRR
                     FHGVDQTRANTAA"
     gene            6927..8783
                     /locus_tag="VM841_06080"
     CDS             6927..8783
                     /locus_tag="VM841_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL89787.1"
                     /translation="MKIRRALGFAVALGMLASPAATRAAQPVKFTDVPFVMDDGVRLT
                     ADVYLPGDGDGPFPCVVELTPYRKEMRASEGVSFLPGAGIALIEVDARGTGGSEGEYD
                     IVFSMREQLDAAAVVDMAADLALPGFPDACLDKVGMYGGSYSGIIQNLVATLPPHLAP
                     AHLAAIAPQRAYGDLYRDIVYHGGQVIGSFGLAWSTATTALYTQPPTNVNTPQGQAAW
                     IDHLTKNDPMMLHYFDAPYADATYTSNHTTPSFTQKLYDDSSSLPRIENLRVPVLQLA
                     GWFDAFTRGSMEMFRQAHEQESDGTGRGPNYVIVGPWNHGQTHFITPEPKPGLRDALA
                     AWYLHWLAGAPAPAFMAGARVQYYMMGTGAHLDATGLWHESESWPPAEVRYQRYYLRE
                     GGVLSPEAPGDDADAQYVYNPAAGTAEIASRWDNALAGAAYIDWDQRTDEPKGLTYTT
                     APFTEPLAIAGTINLHLRATSVGLAGAGEPAALPGLLQTLPPWHDTDFVVKLTDVDTA
                     TGRSTLITQGYLRGSHKAVDGSRTVFAPNGDVIAAFHPHTAASLQPLQPQDTFEIEIW
                     PTAKTFAPGHALRIDIYSADTPNHLNLLKPALNTIHHRVGLESWLNLPVMPA"
     gene            complement(8796..9176)
                     /locus_tag="VM841_06085"
     CDS             complement(8796..9176)
                     /locus_tag="VM841_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HVL89788.1"
                     /translation="MGITYDTGALVAADRGERRMWARHRALLAMRIVPVVPAPVLAQA
                     WRGKPKQALLARLLVGSDIEALTDELARAAGTVAARAGTSDIVDACVVEGAVRRRDLV
                     VTSDASDLTAIASAAGRRLEVERP"
     gene            complement(9176..9451)
                     /locus_tag="VM841_06090"
     CDS             complement(9176..9451)
                     /locus_tag="VM841_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89789.1"
                     /translation="MGLRKKRSISVPPDLDSKIEAAAIEAGMTYSGWLMHAARKEFTI
                     RAGLEAVIAFEREHGRFTAQELAEAAEWAATSVRRGKRTGARQRRTA"
     gene            complement(9528..11153)
                     /locus_tag="VM841_06095"
     CDS             complement(9528..11153)
                     /locus_tag="VM841_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL89790.1"
                     /translation="MKRIAAMIVLVSVTVGFPVRAGLGYEVSVASVEMSDGVALDVSL
                     YTPLAPAPPGGFPLIVRQHGGGSSKDSEYDVRYALKAVHTNRFAVLMYSHRGHGGSGG
                     VFDFFGERSAADVSELLDWVADTVPRIDVSRTGMWGYSQGGALSLMPAMTDPRIKVVA
                     AGNTFDDLNHALNPNDCFKYSFATGIFALAYKSAAARTDDATALRWGAQWYTDTEDLA
                     IPFVDPITGAAHVRSTTHDAALHSPAAHVSTTPGTVYDTGLNVPVFWTNSWEDALFPA
                     DHGLGIVAGLEARNIPTHLWFASGGHEVPETHGPDEAAKELAMLEWFEEFLLGVDHGY
                     ASGARPKVEYAQRVPGGGWTHKTAPTWPVPGDDVTLHAAAGGGLGEVAAAATLGTIVN
                     TVASANLATEPILSNRAGPLVSNVPDSPGSPATLRFATDPVAAPLEFTGAPRVAIRYD
                     STAQRVAQFNVKLWDEHDGRRRMITRGCLSDETPGDGIADFDLWPASHVLAPGHRLVM
                     TVSTVDFPTFKPDTEPQASTLASATLTLNGVSS"
     gene            complement(11212..11877)
                     /locus_tag="VM841_06100"
     CDS             complement(11212..11877)
                     /locus_tag="VM841_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89791.1"
                     /translation="MPRLAPILALAALLAACGGDGKGPDALPSLSAEGRAFRDLGLAG
                     AEATVRIVYRVAAGDETSEVIVAQRPPQRSFRVTEGQTSLIVATEAGDVVTCGQEECF
                     RIPGLGSSIAQAGAAILGPLAQGFDMFHGEQALEGYAPAPGRTIAGREATCGTWKPEF
                     SPDGFTMCLDAKTGIALLYEIASGNIDVTLEATEVGEPGDDDFEPLFPVTDLPTGYVP
                     PSN"
     gene            complement(11881..13257)
                     /locus_tag="VM841_06105"
     CDS             complement(11881..13257)
                     /locus_tag="VM841_06105"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HVL89792.1"
                     /translation="MEQQIVFGDRFLSVELPDRTRILSAGLSTTLEPTDDIEGAVRAA
                     LAEPVDTPPLRELARGKRRVTIAFDDPTVPCFAPVWQTAIPIVLAELEAGGVDRRNVT
                     LLCANALHRQFSHDELGGIIGQDLVREFADRLTCHDAEDPGMIERLGVTPSGFDVELS
                     RHVTDSDLTVYVNASTIRGFSGGWKSICVGLSTYASIRHHHNPDDMSMSLHRNRMHEM
                     LDEMGALVTERLGRERIFKVETILANPLAVARIHAGTVDGTRAVAVETNRAAQGARAT
                     TDKADVVLYGLPDWSPYAAYSHTNPILTLISTGLGYLGGMIEAAGKPGCTVILATPCP
                     DRWDDRAHPSYREVWERVLPVTRDPYEAKRQFEAEYAHHPEYIERYRFHNGFHPVHGI
                     MALFPLKRLKHAGRVFVAGIENPALARHVGYEPFATVEDAIGAALEIHGADADIGLVP
                     YPMAINRQ"
     gene            13343..>14144
                     /locus_tag="VM841_06110"
     CDS             13343..>14144
                     /locus_tag="VM841_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HVL89793.1"
                     /translation="MRHATGAGVAAGLALAALLALPAAAAESTLELKAGGYPVPYLSV
                     PIDASMPGPAIDVGAGDFTVELFLRASPGANTVEACDLTPQWHLGSVLIDRSRAADQG
                     RRGFGLALSRDRLVFGVTGPDGEHASVCGSRRIADGAWHHVAAQRRAADGRITIWVDG
                     ALDASATGPSGDVSYPDGATYPFGCNCSQDPYLFIGAKEHHSAPSFVGRVDELRISSA
                     LRYAGPFTRPARAFTPDAKTAALYHFDEGSGSVARDATGRLDATFGTWT"
BASE COUNT         2158 a         4867 c         4940 g         2179 t
ORIGIN      
        1 cccggatcga tcgggaaccc gcagaactgg cattgcggcc gcccgccgga gcacacgatc
       61 tcggcatgct cggcgagggc cttcatctgc gctcgcgtgg ccacgaaccg cgcgtgcgcc
      121 aggtcctggg cggtctcgtc atcctcgtcc ccgctgccgg cttcctcggc gacgaacacc
      181 atcaggccgg tatcttcctc gacctcgatc gccatcggcc cgatccgcca ctcgggcagc
      241 agcggatctt ccagatccat ctcgtccgac gtcggcggaa gcccctcgtc ctcgatcggg
      301 tcgagcgtct cgagcagttg cgaaagcgtc gtggccagga cctgcacctg ctgcttctcg
      361 gccagtaacg tgacgagcac ctcgtccttg cgggcctgga tgtagaaggt gcggttgccg
      421 ggctcaccga tcgccccggc ggtgatgcgc gaaacgggat ccagctcgaa ggatgtgctc
      481 atttcttcct cgggacgagc tcgtcgagcg agccattgtc gttgaccttc aggatgcgcg
      541 gcgcgccgtc tccgacccac accgcgctca cggaggcggg accgacggcg agccgggcgt
      601 agaggtcgag atggatgccc gcgtaatggg ccagaagcat cttgatcacg tcggcgtgcg
      661 tcacgatcgc cgccatgccc ttcgggtgcc cggccgcgac ctcggcggcg gcggccacga
      721 cccgcgccga cgtttcgcgc agcgcctcgc cgccggggaa gcgtccttcc gacgggtgag
      781 cgatcacgtc gcgccagtgc tgcgtcttgg cgagctgctt gagcggacgg ccttcccagt
      841 cgccgtagcg gacctcgccg aagcccggcc ggatcttgag ccgcatcttc ttgacggccg
      901 cgagcggctt ggcggtctgc actgcccgct cgaccggcga cgagtagagc gcgtcgagcg
      961 ggatcgaggt gaagcgctcg gccatcgcct tggcctggag tacgccttca tcggacagcg
     1021 gaacgctcga ccagccggcc agcttcacgc cggtaagggg cgtcatcgcg tgacgaatga
     1081 gaaacagcag catggccgca gcatatccgc cgagccgctc gcagcgacgg gctaggcctg
     1141 ctcgtcgtag aagagagcga gcgaggcggt gaagccgtgc aggaagacgc ggccgccgac
     1201 cgggccgatc tcgcccgcgc agctcatgcc cgccagcggc gcgcgccaca ccgacgacag
     1261 cacgcccgca tcgtgatccg gcgcgccgaa caggtgcgag ccgcgaccgt tgcaggtgaa
     1321 gagcatcgcg ccggccggcg gtggcacggc ggccgcggcg gcgcgcagat cctcgtcggc
     1381 cgtcgccgcg tcgcgaacgt ggaactgaac ggtctcgccg acgccgacca gatcgccgac
     1441 ggccatcgcg cccgtggtct cgtcggcgcc catgacgttt cggatcagaa agtcgccgcg
     1501 ttcgaactcg gtcttgtact cgtcgacgac gcggccgatg tgcaggccgc gctggatcag
     1561 cgcgcgctcg tcgggcgagg cttcgatcca caggtcgcgc agacgctcga ccggcggcag
     1621 gccggccagc tcgacgatga cgttgcgctg cgcgttggtg acggtcgcgg gcgtgccgat
     1681 cggacggcaa ccttgcgaga cgagcgtgcg gaccttgacg cgcccgcgga tcacgacgcc
     1741 ggccgcgccg ccgttggcga cctcgctgcc ctgccagatc cggtgcccgc cgggagcccc
     1801 ggcgccggag gcgagcccgc ccaccacggt caggccgggc tgctcggtgt tgaccgtgtc
     1861 caggagccgg tccaccggca ccgtgaacgg atcggcgagc atgatcacgg ccgcgccggc
     1921 gggcggcgag ggccagccga gaaacgtcgg cccgtccggc gtctcggtga actccagcga
     1981 gaacggctcg acctcggtgt cgggcagatg ggccgcccag accgccacgg cgggacggtc
     2041 ctcgatctcg cggccgcctt cggccacggt gccgccggtc gcaccgatca tcgcgcccgg
     2101 ctcgagcgcg acgtggaccg cggcggcgat ctcgaccgcg gcgtccgcgt gcgcggccga
     2161 cacgaagatc gcggccaggt cgcacggggc accgctcagg ccgcgcgcgg ccgtcgctgc
     2221 cgcctcggcc gccgccgcca ccccgtccgg gtcggtggac agtccggtcg cgacgacgag
     2281 acggccggtc acttcttcga tccctccggc gggaacgtcg cgcgcgtgtc gggctcggaa
     2341 taggtggtcg cgcccggcga gggccgcgcg gcttcgggga cccagtcgct gtgcgcctgc
     2401 accgggtcct gggtctcgtt ccaatacgac tgcatcgccc aggtgaacgg gttcttcagc
     2461 ttcgacagca gcgtcagcgg aatgtccgcg cgaacctcgc tctccgtgat cttgagcgtc
     2521 gctccttcgg cgggctcgtt gcaaacctcg gtgccggggc acaccgacag gcgccagccc
     2581 ttgatctgct cgcccgctcg gctcggctcg ccttcccgga cgatcgcgac gaagtacgcg
     2641 ggggaggcgg ccttggtgtc ggcgaacaac tgcatgaacc aggccgggcc ttgcagcgga
     2701 tcggtgatgg aagcagactc gtagggcgag cccgcgatcg cgatgcggat cgacagcgag
     2761 tcctcgctag cgtccagccg cacgccggtc acatcggcgg cgtcgggcgg gttcttcggc
     2821 tcgacgccgt cggcgttgag ggccttggca tggtcgccct tgacgtccac gaccagctgc
     2881 gcgcccgaca gcggatcgtt cgtggacgcg tcgccgccgc aggcggcgag cccgagcgcc
     2941 agcaccagcg ccggcgcagc tacttgggcg cgcacgtttc gaccaggtcg cgcgacgggg
     3001 gctgatagaa gctgagtacg acgtgttcgc atcctcggtc ctcgtattcg ggaattttgg
     3061 cgagctgctc ttcgggctgc ccgtcgatcg cgggatgcac gaacagttgc acgccgcggc
     3121 gtacgtgttt cggatcccgt ccgatcgcgg cgcagttgtc gtcgatctgc ttcgacagct
     3181 gcgaccacag ctcgggatcg gcgcccggga cgttccattc gtcggcatgc tcggcgacga
     3241 ccttcagcat cttgggcttc acgccgccga cgacgatcgg cggccgcggc tgctgcaccg
     3301 ccttgggctg cgtgatcgcg tcctgcaatt ggaagtagcg gccgtcgaag gtcgcacgat
     3361 cctgggtcat cagcgccttg atcacgtgca gcgcctcgtc caatcgcgcg acgcgttcgc
     3421 cgggcggcgg aaactcgatg ccaaggccgg tgtgctcggc ttcgtgccag gcggcgccga
     3481 tgcccagctc cagccgtccg ccgctgatgt gatcgacggt gatcgccatg ttcgccagca
     3541 ccgccggatg ccggtaggtg acgccggtca ccatgcagcc gacgcgcgcg cgcttgacca
     3601 gcaccgccat cgccgccagc gtcgtccagc cttccagcgt cggggcctcg tgttcctggc
     3661 tttgcgtgcc cagaccgtag aagtggtcgt agttccagac cgcgtgatag ccggcctcat
     3721 cggccgcggt ccaaaactcg gcgagcgtgg cgtaatcgaa cgtcggagcg agcttcgcgg
     3781 agatcagcat ggcctcgaat gctacccgtc ccgggccgga cctggcagga ggcggctccc
     3841 tcgggcgcga aataccggtc tatgggaatt cttcgtgcga ttcttccgtt ggcgctggcc
     3901 ctgtcggtat ccgcccccgc cgccgccacc gattcgcaga ttcccgtgac cgtctacgcc
     3961 caccggggcg gcgcggcgat cgggccggag aacaccatgg ccgccttcga gcaggccggg
     4021 gcgttgttcg cggcggcggg cgtagacggc tggctcgagc tggacaccca ggcgtcggcc
     4081 gacggcacgc tggtcgtgat tcacgatcac acgctcgacc ggacgaccga ctgcgccgga
     4141 accgtgatcg actggaccta cgacgcctcg tgcaacgccg cgaaacgctg gccgtcgttc
     4201 gggcatcagc cggtcccgaa gctggcggat ctgctggcgc gcggccggca ggcgaccccc
     4261 gcctggggcc tgatgatcga gatcaagaac atcccctacg agaccaactt cgatccggcc
     4321 ggcatgctgg tcgccgactc gctggtcgcg atgatcgacg cgagcggcta tccgaaggac
     4381 cggatcatcg tccagtcgtt ctggccgccg tccctcgacc gggtcgagct gctggatccc
     4441 gaactggaca cgatgctgct gaccacctcg caactgggtt tcatggtctg gcagaacgcg
     4501 ctgtatgcaa ccgcacgcgg ctacgagatc agcgcgccgg aatggcagtc cgcggacttc
     4561 cgcgccgaga cggtcgcggc ggcgcatgcg ctcgggcgcc gcgtcatcac ctggacggtc
     4621 gattcccgcg ccgccggcga cgcgctgatc gcgatgggtg tggacggcat tatcaccaac
     4681 gacccgagga tcttcctatg agacgtcttc tgattctatt gtcggcgctg gcgattgccg
     4741 gcgtgttcgt gcccgccacc gccgccgatc ctgcgtcggg cacgctgtcg gatccgtcgg
     4801 ccaccgttgc gtggacgggc acgcattggc ggaccgacga cgcgccgatc ccggaccagt
     4861 tcgatctgac gatcgatctg ccggccggct actgggacgc caacgacggc ggcgtcgagg
     4921 tcgcgatccg atgggtcagc gaatacaacg tgttcgatct atacgtgctc gacgccggcg
     4981 gcgcgacggt tgcgtcctcg accggctttc cgacctcggc gcactcgctg atcatgcgcg
     5041 agcccgcgaa cggcctgtat cacgtcgtcg tggtcccggc cggcgtgcag gtcgacgagg
     5101 agcggccgta cgagggcatc gcgcaagtcg agctggacac gaccaggacc ggaccgctgg
     5161 ttcccgatct ggtcgcgctg ccgccggccg agttcaagat tgcggccgga acgctgtcgt
     5221 tcatgccggt cgagtatcaa gagacgggcg gctgctatcc cgacgagatg gccgagaacc
     5281 cgtcgctgac gcgctgtctg cgcttcgatg cgggcgtcgc gaacctggga cacggtccgt
     5341 tcgagctgcg actcgacacc acgacgatgg cgggcgagcc cgtcgtgtgg caggacatct
     5401 atgcggccga cggctcgcgc acgaccatcg gcgccggcac ctacacctgg cacgcgacgc
     5461 acgcgcacgt gcactaccag aacttcgtcg agtaccggct gcatcgcgtg ggcgaggacg
     5521 gaacgccggg cgaagaggtc gtcgggtcgc gcaaggccga cttctgcatg atcgacacgc
     5581 gcctgatctg gttcggcgag tacggcaacg gaccgcgcaa tcaccacttc ccgcaatgca
     5641 acatcccggg cggggacggc gccggcccca ccgagatgcg ccaaggaatc gacgtcggct
     5701 gggcggacat ctacacgtgg gatctgcccg gccaatacat cgacatctcg aacgtgccgg
     5761 acggcatcta tgccgtcgtg ctcgaagcgg acccctccgg agtgctcagc cagctcaccc
     5821 gcaccaacga catctcttgg acgaggatcc ggatcgccgg cctttcggtc accgagtgca
     5881 ccgctccccc gcccggctgc tgaacacgaa agcacctact caacgggtat cgggcgccgg
     5941 ggggtatact gccgacggct tcgggatcga cgagccggcc ggcaggccgg ttttttgtgg
     6001 agcgaagccg accactcatg gcaggaagac cgagaaaacc cggcggcagg ccgggagggc
     6061 agcgaggagg gccgcgcaag ggcggggccg cctcgcgggc ggcgcgcgcc caaagccggg
     6121 ccgagcagcg cgaaaagcag ctcgctcgct cgcagaacac caaggaagag cccatcgaga
     6181 tggaaggcat catcgtcgag gcgctcccca acgtcatgtt ccgcgtcgag ctggagaacg
     6241 ggcatatcgt gctcggccac atctcgggca agatgcgcaa gcactacatc aggattctgc
     6301 cgggtgatcg cgtgaagctg gagctatcgc cctacgatct gaaccggggc cggatcacgt
     6361 accgctaccg ctgacccgct aggcagcggt gttcgcgcgc gtctgatcca cgccgtggaa
     6421 gcgccggcga acccgccacg atccgtacga gaaccctccg accgccagcc ctgcccccgc
     6481 gatcgtgacc cacgaaatcg gctgcccttc gccgcgagcg ccggccggcg cctgatccgc
     6541 ggtggtcgtg ccgccgtcgt cgcgcacgca cttggtcgcc ggcccgagcg tgacgccggg
     6601 gacggatccg ggattcgagt cgggctcgga cacgcaggat tcccccgcga acgcgggcaa
     6661 cgacaaggcg agcaccgcca ggatgaccag cagcgcgcgc ccgaataccc cgatacccca
     6721 ccgcatgatg attctcagag agtgtaacgg cctacgtgcc ggacggctat gggcatccac
     6781 cgcgggcaat tgcgtcgcag gcgggcctta cggcatccgg ggcatcccgg gcggcaggat
     6841 tcgatccgga caatgcagaa ccaatcaggc agcgggaccc gccgtccccg tcacgccgga
     6901 tcgatccgac cctggagggg atcttcgtga agatcagacg agcgctgggc tttgccgtcg
     6961 cgctgggcat gctggcttcg ccggccgcga cccgcgccgc gcagccggtc aagttcaccg
     7021 acgtgccgtt cgtgatggac gacggcgtgc gtctgacggc cgacgtgtac ctgcccggtg
     7081 acggcgacgg tccgttcccg tgcgtggtcg agctgactcc ctaccgcaag gagatgcgcg
     7141 cgtccgaggg cgtgtcgttc ctgcccgggg cagggatcgc cctgatcgag gtggacgcgc
     7201 gcggcaccgg cggctcggaa ggcgaatacg acatcgtctt ctcgatgcgc gagcagctcg
     7261 acgcggccgc ggtcgtggac atggccgcgg accttgcgct gcccggcttc ccggatgcgt
     7321 gcctggacaa ggtcggcatg tacggcggct cgtactcggg gatcatccag aacttggtcg
     7381 cgacgctgcc gccgcatctg gcgcccgcgc atctggccgc gatcgcgccg caacgcgcct
     7441 acggcgactt gtaccgcgac atcgtctatc acggcggcca ggtgatcgga tcattcggcc
     7501 tggcgtggtc caccgcgacg accgcgctct acacgcagcc gcccacgaac gtgaacacgc
     7561 cgcaggggca ggccgcgtgg atcgatcatc tgaccaagaa cgatccgatg atgctgcact
     7621 acttcgacgc accctacgcc gacgcgacct atacctcgaa ccacaccacg ccctcgttta
     7681 cgcagaagct ctacgacgat tcctcgtctc tgccgcggat cgagaacctt cgggttccgg
     7741 tactgcaact ggccggctgg ttcgacgcgt tcacgcgcgg cagcatggag atgttccgcc
     7801 aagcccacga gcaagagtcc gacgggaccg gccgcggccc caactacgtg atcgtgggtc
     7861 cgtggaatca cggacagacg cacttcatca cgcccgagcc caagccgggc ctgcgcgatg
     7921 cgctggccgc ctggtatctg cattggcttg ccggcgcgcc cgcgcccgcg ttcatggccg
     7981 gcgcgcgcgt gcagtactac atgatgggaa ccggcgcgca tctggacgcg accgggctgt
     8041 ggcacgaaag cgagtcgtgg ccgccggccg aggttcgcta tcagcgctac tacttgcgcg
     8101 agggcggcgt cctatcgccc gaggcgcccg gcgacgatgc ggacgcgcag tacgtgtaca
     8161 acccggcggc cggcacggcc gagatcgcct cgcggtggga caacgcgctc gccggcgccg
     8221 cgtacataga ctgggatcag cgcaccgacg agccgaaggg cctgacgtat acgaccgcgc
     8281 cgttcaccga gccgctcgcg atcgcgggaa cgatcaacct gcaccttcgc gcgacgtcgg
     8341 tcgggcttgc cggcgcgggc gagcctgcag cgcttccggg gctgctgcag acgctcccgc
     8401 cgtggcacga caccgacttc gtggtgaagc tgaccgacgt ggatacggcg acggggcgct
     8461 cgacgctgat cacgcagggg tatctgcgcg gctcgcacaa ggcggtcgac ggctcgcgca
     8521 cggtgttcgc gccgaacggc gatgtgatcg cggcgttcca tccgcacacg gccgcatcgc
     8581 tgcagccgtt gcagccgcag gacacgttcg agatcgagat ctggccgacc gccaagacgt
     8641 tcgcgccagg tcacgcgcta cggatcgaca tctactcggc cgacacgccg aaccatctga
     8701 acctgctcaa gcctgcgctc aacacgatcc atcaccgcgt gggtctcgag tcgtggctga
     8761 acctgccggt gatgccggcc tgatccggat cgccgttagg ggcgctcgac ttccaggcgc
     8821 cgcccggccg cggatgcgat ggccgtcagg tcgctcgcgt ccgaagtgac gaccaggtcg
     8881 cgccggcgca cggcgccttc gaccacgcac gcgtccacga tgtcggacgt gccggcacgc
     8941 gccgccacgg ttccggccgc gcgggcgagc tcgtcggtga gcgcttcgat gtcggatccg
     9001 acgagcaggc gtgccagcag agcctgcttg ggtttcccgc gccacgcttg cgcgaggacg
     9061 ggtgccggca cgaccggaac gatgcgcatc gccagaagcg cgcgatgccg cgcccacatg
     9121 cgccgctcac cgcgatcggc cgcgacgagc gcgcccgtgt cgtaggtgat gcccattacg
     9181 cggttcggcg ctgccgcgcg ccggtgcgtt tgccgcgccg gaccgaggtc gcggcccatt
     9241 cggcggcctc ggccagctct tgggccgtga agcgtccgtg ctcacgctcg aacgcgatga
     9301 cggcctccag accggcccga atcgtgaact ctttgcgcgc ggcatgcatc agccacccgc
     9361 tgtaggtcat gccagcctcg atggccgccg cctcgatctt cgagtccagg tcgggcggaa
     9421 ccgagatcga ccgcttcttt cgcagcccca tggacccgat ggtagcacag cggtaggaca
     9481 cgattgggtc gttcgggtct ttcgaccggc gatcgtggcg gcgccaccta ggatgacacg
     9541 ccgttcaggg tgagcgtcgc cgacgcgagc gtggatgctt gcggctcggt gtcgggcttg
     9601 aaggtcggga agtccaccgt ggagacggtc atcaccagac ggtgtcccgg cgcgagcacg
     9661 tgcgaggcgg gccagaggtc gaaatcagcg atgccgtcac cgggggtttc gtcgctgagg
     9721 catccgcggg tgatcatccg ccgccgccca tcgtgctcgt cccacagctt gacgttgaac
     9781 tgggccacgc gctgcgcggt gctgtcgtag cggatcgcca cccgcggcgc cccggtgaac
     9841 tcgagcggcg cggcgaccgg gtcggtggcg aaccgcagcg tcgcgggcga gcccggcgag
     9901 tcgggcacgt tcgacacgag cgggccggcg cggttcgaca ggatcggctc ggtggcgagg
     9961 ttcgcggacg cgaccgtgtt gacgatcgtg ccgagcgttg ccgcggccgc gacctcaccc
    10021 aggccgccgc cggccgcggc gtgcagcgtc acgtcgtcgc ccggcaccgg ccaagtcggc
    10081 gcggtcttgt gcgtccagcc gccaccgggc acgcgctgcg cgtactccac cttcgggcgg
    10141 gcgccgctcg catagccgtg atcgacgccg agcaggaact cttcgaacca ctccagcatc
    10201 gccagctctt tcgcggcttc gtcgggtccg tgggtttcgg gaacctcgtg gccacccgac
    10261 gcgaaccaca ggtgcgtcgg gatgtttcgc gcctccaggc cggcaacgat gccgagcccg
    10321 tgatcggcag gaaaaagcgc gtcctcccac gagttcgtcc agaagaccgg aacgttcaag
    10381 ccggtatcgt agaccgtgcc gggcgtggtc gagacgtgcg cggcgggcga gtgcagcgcg
    10441 gcatcgtgtg tggtcgagcg cacgtgcgcc gcaccggtga tcgggtcgac gaacgggatc
    10501 gcgaggtcct cggtgtcggt gtaccactga gcgccccatc gcagcgcggt cgcatcgtcg
    10561 gtgcgtgcgg cggccgactt gtaggcgagc gcgaaaatgc cggtggcgaa cgagtacttg
    10621 aagcagtcgt tcgggttgag cgcgtggttg aggtcgtcga acgtgttgcc cgccgccacg
    10681 accttgatgc gcgggtcggt catcgccggc atcagcgaca gcgcgccgcc ctgcgaatag
    10741 ccccacatgc cggtgcggct cacatcgatc cggggcacgg tatccgcgac ccagtcgagc
    10801 agctcggaca cgtccgcggc cgaccgctcg ccgaagaagt cgaacacgcc gccgctgccg
    10861 ccgtggccgc gatgcgagta catcaggacc gcgaaccggt tcgtgtgaac ggctttgagc
    10921 gcgtagcgca cgtcgtattc gctgtccttg ctcgatccgc cgccgtgctg ccgcacgatc
    10981 agcggaaagc cgccgggcgg cgccggcgcg agcggcgtgt acagcgacac gtccagcgcg
    11041 acgccgtcgc tcatctcgac cgacgccacc gacacctcgt agccgaggcc ggcgcggacg
    11101 ggaaacccga cggtgacgga cacgagcacg atcatggctg cgatgcgctt catgagatcc
    11161 ctccccaccc tcgacttcgg caccgcggct cgcggttcct gccgcgcgcc gctaattgct
    11221 gggcgggacg tagccggtcg gcagatcggt gacggggaac agcggctcga agtcgtcgtc
    11281 gcccggctcg cccacctcgg tcgcctcgag cgtgacgtcg atgttgccgc tcgcgatctc
    11341 gtagagcagc gcgatgccgg tctttgcgtc caggcacatg gtgaagccgt cgggcgagaa
    11401 ctcgggtttc catgtgccgc aggtcgcttc gcggccggcg atggtgcggc ccggcgcggg
    11461 tgcgtatcct tcgagcgcct gctcgccgtg gaacatgtcg aacccttgcg cgagcggccc
    11521 gaggatggcg gcgcccgctt gcgcgatgga cgatccgagc ccggggatcc ggaagcactc
    11581 ctcctggccg caggtgacta cgtcgcccgc ctcggttgcc acgatcagcg atgtctgtcc
    11641 ttcggtgacg cggaacgagc gctgcggcgg ccgctgcgcc acgatcacct cgctggtttc
    11701 gtccccggcg gccacgcggt agacgatgcg cacggtggcc tcggcgccgg cgagaccgag
    11761 atcccggaac gcgcggcctt ccgccgacag tgacggcagc gcgtcggggc ctttgccgtc
    11821 gccgccgcat gccgcgagga gcgcggcgag cgcgaggatc ggtgcgagac ggggcatcgg
    11881 ctattgacgg ttgatggcca tagggtacgg gaccagcccg atgtcggcat cggctccgtg
    11941 gatctcgagc gcggcgccga tcgcatcctc gaccgtggcg aacggctcgt agccgacgtg
    12001 gcgcgcgagc gcggggttct cgatgcccgc gacgaaaacg cggcccgcgt gcttgagacg
    12061 cttgagcggg aacagcgcca tgatgccgtg caccggatgg aacccgttgt ggaagcggta
    12121 gcgctcgatg tattcgggat ggtgcgcgta ctcggcttca aactgccgct tggcctcgta
    12181 cgggtcgcgg gtgacgggca gcacgcgctc ccagacctcg cgatacgacg gatgtgcgcg
    12241 gtcgtcccag cggtccgggc agggcgtggc gaggatgacc gtgcatccgg gcttcccggc
    12301 cgcctcgatc atgccgccga gatagccgag cccggtcgag atcagcgtca ggatcgggtt
    12361 ggtgtgcgag tacgcggcgt aggggctcca gtcgggaagg ccgtagagca ccacgtcggc
    12421 cttgtcggtc gtggcgcgcg cgccttgcgc ggcgcggttg gtctcgaccg cgacggcgcg
    12481 ggtgccgtcc accgtgccgg cgtggatgcg cgcgaccgcg agcgggttgg ccaggatcgt
    12541 ctcgaccttg aagatccgct cgcgcccgag ccgctcggtc acgagcgcgc ccatctcgtc
    12601 gagcatctcg tgcatgcggt tgcgatgcag cgacatcgac atgtcgtcgg gattgtggtg
    12661 atgacggatc gaggcgtagg tggacaggcc gacgcagatc gacttccagc cgccgctgaa
    12721 gccgcggatc gtcgaggcgt tgacgtagac ggtcaggtcc gagtcggtga cgtggcggct
    12781 gagctcgacg tcgaagccgc tgggggtcac gccgagccgc tcgatcatgc cggggtcctc
    12841 ggcatcgtgg caggtcagcc ggtccgcgaa ctcgcggacc agatcctggc cgatgatgcc
    12901 gccgagctca tcgtggctga actggcggtg cagcgcgttc gcgcacagca gcgtcacgtt
    12961 gcggcggtcg acgccgcccg cctccagctc ggcgagcacg atcgggatgg cggtctgcca
    13021 cacgggcgcg aagcacggca cggtcggatc gtcgaaagcg atggtgacgc gacgcttgcc
    13081 gcgcgcgagc tcgcgcagcg gcggcgtgtc caccggttcg gcgagcgcgg cccgtacggc
    13141 gccttcgatg tcgtcggtcg gctcgagcgt ggtcgacagg cccgccgaca ggatgcgcgt
    13201 ccggtcggga agctcgaccg acaggaagcg gtctccgaag acgatctgct gctccacggc
    13261 gcggagcgta cgtcatccgg acgggggacg aaaggggcaa ggaagtcaag catggggcgc
    13321 ggcgcgccga aactcaatca ggatgcgtca tgcgacgggc gccggcgtcg cggcgggcct
    13381 ggcccttgcc gcgttgctcg cgctgcctgc ggccgcggcc gaatcgacac tcgagctcaa
    13441 ggccggcgga tatccggtcc cctacctgtc ggtcccgatc gacgcgtcga tgccgggacc
    13501 ggctatcgat gtgggcgcgg gcgacttcac cgtcgagctg ttcctgcgcg cctcacccgg
    13561 cgccaatacc gtcgaggcct gcgacctgac gccgcaatgg catctggggt cggtgctgat
    13621 cgaccgtagc cgcgcggccg accagggacg ccggggcttc ggccttgcgt tgtcacgcga
    13681 tcggctcgtg ttcggcgtga cgggacccga cggagagcac gcgagcgttt gcggctcaag
    13741 gcgcatcgcc gacggagcct ggcatcacgt cgcggcgcag cgacgcgcgg ccgacgggcg
    13801 aatcacgatc tgggtggacg gcgccctcga cgcgtcggcg accggaccgt cgggtgacgt
    13861 gtcgtatccc gacggcgcca cgtatccgtt cggctgcaat tgctcgcagg atccgtacct
    13921 gttcatcggg gcgaaggagc atcactccgc cccctcgttc gtggggcgcg tcgacgagct
    13981 acggatctcg agcgcgctgc gctacgcggg tccgttcacg cggcctgcgc gcgcattcac
    14041 gccggacgcg aaaacggcgg cgctgtacca cttcgacgag ggctcgggca gcgtcgcgcg
    14101 agacgcgacc ggccgcctcg atgcaacgtt cgggacctgg acgg
//
LOCUS       DATLFO010000159         5379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_73175, whole genome shotgun sequence.
ACCESSION   DATLFO010000159 DATLFO010000000
VERSION     DATLFO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73175"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VM841_06115"
     CDS             <1..1335
                     /locus_tag="VM841_06115"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVL89794.1"
                     /translation="VYQRLISWTKPYAVEERGTRAYERYDWNSLIAVEPENRSLVAPF
                     MDGTSYFPARAEMEQGLLEFASRTGLAVNYGCRWEATRREDEGLVLETSHGEYRSPIV
                     IFAIGATRPWKPAIPGLEDVPHYVEAKPARHYAGRRVFLIGKRNSGFELADGLLPWAR
                     QIILASPRPASLSVTTRSLVGARARYVQPYEDHVLAGGNVVLDVAIRSVERGGDGYRV
                     HAVGTTNPGDFVLTVDDVIAATGFEVGLGDLPGLGVATFNQGRLPAQTPFWGSASVPG
                     IYFAGSATQAAAGLRKYGISSNSAAVHGFRYNAGVLVRHIAERHFGMMFDDPVMPPDG
                     LIEHLLHAATKAPELWNQPSYLAHVVELGARPGPVARGITPLAHFVDANGPDAVAVTV
                     ESDASGDIHPAVYVRSRGTVSEHLLPSAPFHDYETEDHRSLLRAVLDPVTRG"
     gene            complement(1336..3957)
                     /locus_tag="VM841_06120"
     CDS             complement(1336..3957)
                     /locus_tag="VM841_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL89795.1"
                     /translation="MAAPTGTVTFLFTDIEGSTKLLHALGDGYAELLDDHHRLMREVF
                     LSDGFEVGTEGDAFFVAFQSATDAARAAAAAQRRLAAHRWPGGVEVRVRMGLHSGEAK
                     QVGDNYGGIDVHRAARIAGAAHGGQVICSDATRGLIHGSLGDDITLLDLGTHRLKDLE
                     RPEHLFQLCVAGLPVEFPPLRTLDARPTNLPDFLGPFVRRDREVAEIELLVSDRRLVT
                     LTGPGGTGKTRLALKVAADLLDRFPDGVFLVLLAPVSDVTLVAPRIAQVLGVMEHGMQ
                     PIEERLRDFLADKHMLLVLDNFEQIIAAGPVVSELLSAAPKIHLVVTSRAPIRIYGEQ
                     EYPVPPMVVPDPGRGASFEELAAAESVELFAQRARSVDPRFELTPENAAVVAAICARV
                     DGLPLAIELAASRVKLFTPADILKRLEKSLSLLSGGARDLPSRQQTLRGAIAWSYDLL
                     GESSRAFFVRLGIFVGGCSLEAAERVVADDLEATALTELETLVDNNLVRLVAGESGET
                     RFRMLQTIREFALEVLEKGADLEHVEERHALFFLKIAEQGESEFTKDQRWGDLLTDEH
                     DNLRAALRWALRRNRTDIGLLIASALWRFWQVRGHLAEGRTWLTELLAAHPAPDGNRA
                     KGLTALGSVTYWQNDFESCAAHYRESLEIYRALDDRVAMAGALYNWAFVALVARRLDE
                     ARERYEESASLYRELGDDHGLANATWGLAMAALLGEDGAQARALAEVAARLYAEQGDW
                     FGESLTDSVFSQVAKLEGDLARAREIMLKQVDGAESSGDLTALASLCDMVADIDLLEG
                     NAVRGLVLAGAGESLRQKAGGGAPPPLTLISADPRELARRYLTEHEIERAWNEGLAMS
                     PAEVIGYVKKDPEADGS"
     gene            complement(3963..>5379)
                     /locus_tag="VM841_06125"
     CDS             complement(3963..>5379)
                     /locus_tag="VM841_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL89796.1"
                     /translation="SYIYYAYLLSLSSLLILAGALTDYHGRKRMFLLGLAGFGTTSIL
                     CGVAPNMEFLIAARVLQGAAGAMLVPGSLAIITATFEGEEQGRAFGVWAGASAATTIL
                     GPVVGGALVAYISWRAAFLVSVPVIAFAAWVTARYMAETRDEESSGRFDFVGAAIVAL
                     SIGGLTFGTIRGQAQEWQGVAPYVALAVGAVSMIAFPIVMRRSDHPLVPLDLFKSRNF
                     TVTNISTLLIYGALYVFVQFLALYSIGVLGYNEIGFGIATIPTTVFLALFSTRFGALA
                     ARYGPRRFMTLGPVVLALGLLWLTRIPSHSAAWRPDVADLSTFVPSTGYLTDVLPALV
                     VFGIGLMIMVAPLTTALMRSVPVRNSGVASAFNNAISRVGPQLAGALIFVFISTTFYA
                     SLNDELPNEATPRVREQVSPLNPPPADADPALHKAAAEASTDAFHVAMLIAAGMCLTG
                     AAVNGLGIIDPAAAQDTTAGA"
BASE COUNT          929 a         1758 c         1821 g          871 t
ORIGIN      
        1 gtctaccagc gcctcatcag ctggaccaag ccgtacgccg tcgaggagcg cggcacgaga
       61 gcctacgaac gctacgactg gaacagcctg atcgcggtcg agccggagaa ccgctcgctg
      121 gtggcgccgt tcatggacgg cacttcttac ttcccggcgc gcgcggagat ggagcaaggt
      181 ctcctggaat tcgcctcccg caccggactc gcggtcaatt acggctgcag gtgggaggcc
      241 acgcgccgag aagacgaggg gctcgtgctg gaaacctcgc acggagagta ccggagcccg
      301 atcgtgatct ttgcgatcgg ggcgaccagg ccgtggaagc cggccatccc ggggttggag
      361 gacgtccccc actacgtgga ggcgaagccg gcccgtcact acgcgggccg gcgcgtcttt
      421 ctgatcggca agcgcaactc gggtttcgag ttggccgacg gtctactgcc gtgggcgcgc
      481 caaatcatcc tcgcgtcgcc ccggcctgcg tctctctcgg tcacgacgcg ttcactggtg
      541 ggcgcgcggg cgcgttacgt gcagccctat gaagaccacg tcttggccgg cggcaacgtg
      601 gtgctcgacg tggcgatccg gagcgtggag aggggtggcg acgggtaccg ggttcatgcc
      661 gtcggaacca caaaccccgg cgacttcgtc ctcaccgtag acgacgtgat cgcggccacc
      721 ggcttcgagg tcggcctcgg ggacctgccg ggcttgggcg tggcgacgtt caaccaaggc
      781 cgtctcccgg cgcagacccc tttctggggc agcgcctcgg tgccgggcat ctatttcgcc
      841 gggtccgcga cccaggccgc tgccggcctc aggaagtacg ggatctcgtc gaactccgcc
      901 gcggtgcacg gcttccgcta caacgcgggc gtcctggtcc gccatatcgc agaacgccat
      961 ttcggcatga tgttcgacga tccggtcatg ccgcccgatg ggttgatcga acacctgctg
     1021 cacgcagcga ccaaggcgcc cgagctgtgg aaccagccgt cgtatctggc gcacgtcgtg
     1081 gaactgggcg ctaggcccgg cccggttgcc cgcggcatca cgccgctcgc ccatttcgtc
     1141 gacgcgaacg gtccggacgc ggtggcggtg acggtggaga gcgatgcgtc cggagacatc
     1201 cacccggcgg tgtacgtccg ctccagaggc acggtgtccg agcacttgct gccgtcggcg
     1261 ccgttccacg attacgagac cgaggaccac cggtcgttgc tccgagcggt actcgatccg
     1321 gtcacccgag gttgactagc tcccgtcggc ctccgggtcc ttcttgacgt aaccgatcac
     1381 ctccgccggc gacatcgcca ggccctcgtt ccaggcccgt tcgatttcgt gctctgtgag
     1441 gtagcggcgg gcgagctcac gcgggtcggc cgatatcagc gtcagcggag ggggcgcgcc
     1501 ccctccggcc ttctgtcgca ggctttcgcc tgcgcctgcc agaacgagac cgcgcaccgc
     1561 gttcccctcc aacagatcga tgtcggcgac catgtcgcac agcgacgcca gcgccgtgag
     1621 atcgccggag ctctcggcac cgtccacctg tttcagcatt atctcccggg cgcgggcgag
     1681 atcgccctcg agcttcgcaa cctgagagaa gacggagtcg gtcagggact cgccgaacca
     1741 gtcgccttgt tcggcataaa gacgggcggc tacctcggcc agggcgcgtg cttgcgcccc
     1801 gtcttctccc aggagcgcgg ccatcgccag accccaagtc gcgttcgcca ggccgtggtc
     1861 gtcgcccaac tcccgataca gcgacgcgct ctcttcgtag cgttctcgtg cctcgtcgag
     1921 gcgacgcgct acgagcgcca cgaaagccca gttataaagc gcgcccgcca tcgccacgcg
     1981 gtcgtcgagc gctcggtaga tctcgaggct ttctcggtaa tgcgcggcac acgactcgaa
     2041 gtcgttttgc cagtaggtca cgctcccgag cgcagtcagg cccttggccc ggttcccgtc
     2101 gggggcggga tgagcggcca gcaactccgt gagccaggtg cggccttccg cgaggtggcc
     2161 ccggacctgc caaaagcgcc acaatgccga ggcaatgaga agtccgatgt cggtgcggtt
     2221 tcgtcgcagc gcccaccgga gggcggcgcg caggttatcg tgctcgtcgg tcagcagatc
     2281 tccccagcgt tggtctttgg tgaattcgct ctcgccctgc tcggcgatct tcaggaagaa
     2341 gagggcatgc cgctcttcga cgtgctcgag gtctgcccct ttttcgagta cctcgagggc
     2401 gaactcgcgg atcgtctgca gcatgcggaa gcgcgtctct cccgattcgc ctgccacaag
     2461 gcgcacgagg ttgttgtcga cgagcgtttc cagctccgtc aatgcggtgg cttcgagatc
     2521 gtcggccacc acacgttcgg cggcttcgag cgagcacccg ccgacgaaga tgcccagccg
     2581 aacgaaaaac gcgcgcgacg attcaccgag caggtcgtag ctccacgcga tcgcgccacg
     2641 cagcgtctgc tgtcgtgacg gaaggtcccg ggccccgccg ctcagcagtg acaggctctt
     2701 ttcgagccgc ttgaggatgt cggcgggggt gaacaatttg acgcgggatg cggccagctc
     2761 gatggcgagg ggcagcccgt cgacgcgtgc gcagatggcc gccaccaccg cggcattttc
     2821 gggcgtcagc tcgaagcgcg gatcgacaga ccgtgcgcgc tgcgcgaaca gctcgacgga
     2881 ctcggccgcg gcaagctcct cgaaggacgc gccgcgcccg ggatcgggca cgaccatggg
     2941 ggggacggga tactcctgct ccccatagat cctgatcggc gcccgactcg tgaccaccaa
     3001 gtggattttg ggcgccgccg acagcagctc ggacaccaca ggaccggctg cgatgatctg
     3061 ctcgaagttg tcgaggacca ggagcatgtg cttgtcggcg agaaagtctc ggagtctttc
     3121 ctcgatcggt tgcatgccgt gctccatcac gccgagaacc tgtgcgatgc gaggcgcgac
     3181 gagcgtcacg tcgctcaccg gggccagcag gacaagaaaa acgccatctg ggaaccggtc
     3241 gagcaggtcg gccgcaacct tgagggcaag acgcgtcttt ccggtgccgc cgggaccggt
     3301 gagggtcacc aacctgcggt ctgataccag caactcgatc tcggctacct ctcgatctcg
     3361 gcgtacgaac ggcccgagaa aatccgggag gttggtcggg cgggcgtcga gcgtgcgaag
     3421 cggaggaaat tcgacaggca gtcctgcgac gcacaactgg aacaagtgct ccggccgctc
     3481 caggtccttt aggcggtggg tcccaaggtc gagcagcgtg atgtcgtcgc cgagggagcc
     3541 gtgtataagt ccgcgggtag cgtcggaaca gatgacctgt ccgccgtggg cggcgccggc
     3601 aatgcgcgcc gcgcggtgga catcgattcc gccgtagttg tcacccacct gctttgcctc
     3661 gccactgtgc aggcccatgc ggaccctgac ttcgacgccg cccggccatc ggtgtgccgc
     3721 cagccggcgc tgcgcggcgg ccgccgcgcg cgccgcgtcc gtcgcgctct gaaatgcaac
     3781 gaagaaagcg tcgccttcgg tgccgacctc gaagccgtcc gaaagaaaga cttcccgcat
     3841 caaccggtga tggtcgtcca gcaactccgc gtaaccgtcg ccgagcgcat gcaggagctt
     3901 ggtggagcct tcgatgtcgg tgaagagaaa cgtcaccgtg ccggtcggag ccgccatggc
     3961 cgtcaagccc ccgcggtcgt atcttgggcg gcggccggat cgattatgcc gagcccgttg
     4021 accgcggctc cggtgaggca catgccggcg gcgatgagca tcgcgacgtg gaaggcgtcg
     4081 gtggacgcct ccgcggcggc cttgtggagc gcggggtcgg cgtcggccgg gggcggattg
     4141 agcgggctca cctgctcgcg caccctcggc gtggcttcgt tcgggagttc gtcgttgagt
     4201 gacgcgtaga aggtagtgga gatgaacacg aagatcaggg ccccggcgag ttgtgggccg
     4261 actcgcgaga tcgcgttgtt gaaggccgac gccacgcccg agttgcgcac ggggaccgat
     4321 cgcatgagcg cggtcgtcag gggcgccacc atgatcatta gcccgatgcc gaacacgacg
     4381 agcgccggaa gcacatccgt caggtatccg gtcgatggaa cgaacgtcga gaggtccgcg
     4441 acgtccggtc tccatgccgc gctgtgggac gggatgcggg tcagccacaa aaggccgaga
     4501 gcaagaacga ccggaccgag ggtcatgaag cggcgcgggc cgtagcgggc ggccagcgcg
     4561 ccgaagcgtg tcgagaagag cgcgagaaag accgtcgtcg ggatcgtggc gattccgaac
     4621 ccgatctcgt tgtatcccag cacgccgatg ctgtagaggg cgaggaactg aacgaagacg
     4681 tagagggcgc cgtagatcag gagcgtcgag atgttcgtca ccgtgaagtt gcgcgatttg
     4741 aaaaggtcga gcgggacgag cggatgatcg gatcgccgca tgacgatcgg aaacgcgatc
     4801 atcgagaccg ctccgacggc gagcgcgacg tacggagcca cgccttgcca ttcttgcgcc
     4861 tgccctcgga tcgtgccgaa ggtgaggccg cctatggaga gcgcgacgat cgccgctccg
     4921 acgaagtcga agcgacccga cgattcctca tcgcgcgtct cggccatata cctcgccgtc
     4981 acccacgccg cgaacgcgat caccgggacg ctgaccagga atgcggcgcg ccacgagatg
     5041 taggcgacca gagcgccacc caccacgggc ccgaggatcg tcgtggccgc ggacgcgccc
     5101 gcccataccc cgaaggctcg tccctgctcc tccccttcga aggtagccgt gatgatcgcc
     5161 agtgatccgg gcacgagcat ggcgccggcc gcgccctgca ggacgcgcgc tgcgatcaaa
     5221 aattccatgt tgggcgccac gccgcaaagg atggaggtgg tgccgaagcc ggcgagaccg
     5281 agtaagaaca tccgtttccg tccgtggtag tcggtgagcg cgccggccaa gatcaacaag
     5341 gagctgagcg acagcaggta ggcgtagtag atgtagctc
//
LOCUS       DATLFO010000160         8632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_84706, whole genome shotgun sequence.
ACCESSION   DATLFO010000160 DATLFO010000000
VERSION     DATLFO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84706"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VM841_06130"
     CDS             complement(<1..302)
                     /locus_tag="VM841_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89797.1"
                     /translation="MASPERTIAALAEAVIPSPPDDQTAGAGDVAAERFVLHYLTFLD
                     PSLPGLLAAALDGVAAARRSVPFEAGLFEAMDSEERLRALRLLGEHEDPGLRDLAEL"
     gene            complement(310..609)
                     /locus_tag="VM841_06135"
     CDS             complement(310..609)
                     /locus_tag="VM841_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89798.1"
                     /translation="MKIKGRCTVCGRDFPADLVIEGETVGRCPFCGTPMDADYSGNFV
                     SALASVQRLGSALEAALDQLDALGPVFEMDENSLLDPLRAALAQRSKVTSARQSA"
     gene            complement(606..980)
                     /locus_tag="VM841_06140"
     CDS             complement(606..980)
                     /locus_tag="VM841_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89799.1"
                     /translation="MKVLTVSEDPARHRFVAGALGPAYDFVEAANGRDALARAETDPF
                     DLVITDETAEPFGAFGLSRELKMMIDPPAVIVLLERAGDDWLARWSGADRWLVRPIDS
                     FALADAAKALTHRGATVTRRSE"
     gene            1139..1930
                     /locus_tag="VM841_06145"
     CDS             1139..1930
                     /locus_tag="VM841_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HVL89800.1"
                     /translation="MEKSMGQRLVAEFVGTLTLVFIGAGAVLAASIGLPGAGLVTYAL
                     ATGLAIAVMVSALGHVSGGHFNPAVTIGIWIAQKIKSSDAIAYVVAQLAGATAGAALL
                     RFALPETLWRAGALGTPQVTTGVTNGQAVLIEAILAFFLVWVVFGSAVDAEGSFGKIA
                     GLGIGLVYTMDIMMAGPLTGGALNPARHVGPALMSGTWKGWWVYWIGPVAGGIVAAAL
                     YDGVILRKKEVGLAPDEAPHGFGAHGDEELPPLADPPLADPPVGE"
     gene            complement(1996..4335)
                     /locus_tag="VM841_06150"
     CDS             complement(1996..4335)
                     /locus_tag="VM841_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89801.1"
                     /translation="MVLLAAVVLASVPQPGFAAHALQTLDVEPETGAAATGSPYQLTA
                     TLGAPATTSTVVYFELTGGPNDADGYTPETPDMSCTVAITMSSCVTQYTPAFANVDSI
                     RAWVHHSGAAPQSEPDHGEGINELLVPGDVAEPDRTDVVSVVVGTGGGGGGAQVLDCD
                     DEAPAGNGDDIEVVPTTGSEAATHVTYTCKVTVSGTGVANVTIDAENLTAGVNDADAG
                     KVAPPDYGCVTTAAGTCTFDVPLQTGTLQAGTALICAWIDGDNDDEVNDATAGDGGNC
                     NEPVDAAENDNGTDVMQVTWQTRVVTTIDILNEDDANVLPGEPNHTVNVKAIDQFGDG
                     IGNVNIDLYVLGGPNAQTGSTVNKECTTLANGTCGITYASNGNNGVDRFCAWQDTGTA
                     DFYAPGGAVGDGGACATEPDAPGEDGGNALTDRGQVTWSGGTSVATQLEVTPETPPGG
                     NVAQGTHYGLTATVKDENLQPMSGVLVSFELIGAGDPDSGDSPASPDRQCTTGDNGTC
                     SLTGPPGAIGSSTTFTSETNGTTTVRAWVHGMPADTAEGPDAGEPPDEPAGGTNVPGN
                     IVEPDTTDVVLVRWGRSVTAITASITPDLGVYGIDPQMTGTLTGDGSPLANKTVSIRR
                     RVVGQSGFIQLGSVQTAADGTFQFAETNPSASSDYQASYAGDGENQPSSSPLLRVGIR
                     PGVIFNSSASSLPRGVNVQLSGAVIPAHPGKKVWLQRLDGPSGTWVWVQEIILDGNGR
                     YQFQFKSNGNFCLLFRIAYPTQDTDHLWNVSRNQRVCWS"
     gene            4591..5133
                     /locus_tag="VM841_06155"
     CDS             4591..5133
                     /locus_tag="VM841_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HVL89802.1"
                     /translation="MNAIVDQPSRSGISVFGPSSERLDRLGEWFAAEYEPLLRFAYFV
                     TGDHEAARDTVQDAFIKIYKAGGRVEHEGFPAYARRTVANLGRSRFRRQRSERAAIAG
                     LPRVAEGVRDSDPVERDEVWAAILRLSKQQRAVVALRFYEHMTESQISATLGVSAGTV
                     KKQLSRALDRLSIELEGRPR"
     gene            5130..6260
                     /locus_tag="VM841_06160"
     CDS             5130..6260
                     /locus_tag="VM841_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89803.1"
                     /translation="MTDRRRDIESRVEEALMRRSTDVEPQPGADWDGVRGGVHRAQRV
                     RVAVSTVAALALIGAGVFVVPKLMGRDSGITPVKPGPSVTSPTPTATPPAVPADWQVL
                     RYDPQGYWLTVPASWRTGWFEGHAEYRPKGLPSAVQGEDTFFVETFSSGGVAEPEGDP
                     EALTINGAPAKRWETPSESGAYTVTFDITLPCYAGPGSSAHIDCALVPLRVVVYASTD
                     ALWKEHGKTGEEIARSITAAERTMPVQDGQLYRTFQGGSTVAWDAKTWAVVEFMESRI
                     FGDTERGDRFLTANAKKQYETGEGGLDRYGPDSKFRLVTYTIERREGADANSDEFTVR
                     ITEQRLGAEPVNEIIETLGVGPTESNHVFVPYQIRFATRSEA"
     gene            complement(6267..7241)
                     /locus_tag="VM841_06165"
     CDS             complement(6267..7241)
                     /locus_tag="VM841_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HVL89804.1"
                     /translation="MLKAFTHRPGEGWSELGDLRRISEAIAEPGELLWAYADIGSLTS
                     EDAAQIAEEFGLHSLAVEDALNARQRPKVESYDNHLFVVVHQLDEVAGQLEARQIACF
                     VGKDYVLTIHDGADRLVERAVARTRRTEREPGRGPSWIMHALVDAVVDEYQAIADAIE
                     AEVEELEEGTLANPVGGSGQQVYTIKQRIARMRRYVMPGSRALAGFVEAARAGGGASE
                     TTAAYFRDVLDHTLRIGDQIRNVEDLVNAMMEFRRAEQANALNEVTKKLTGWAAIIAI
                     PTLIASVYGMNFALFPREGSTTGFFVAVSAMGAAAAALYVFFKRRRWL"
     gene            7423..>8632
                     /locus_tag="VM841_06170"
     CDS             7423..>8632
                     /locus_tag="VM841_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89805.1"
                     /translation="MSRTRKHSVPVRLLLALAIAAGMAAAPGGAVVPLRLGLVLPVDC
                     NTSVDDIAAVQDAVDAAGDGDTIQLSGVCDFSGAKPHGGDIASITATAVLIRAGSPFS
                     AGLTIESAEDARATVLGSGTQTAFMVAPGNDNVTIRGLNLLGFARPIVVWSASNTTIG
                     DAAATSAPSADGNRIAGDAMNSAVLAIGNNKDASGATPLTAVSYGSGSARSALLANNG
                     RLTNLRVLGNYITYGAVDAPDGQTRDVVAIDVRQRNQGNVDGVEIAHNAVGFLTASFP
                     SFNINAVRVHAHSGDPNYHLSNVSIRNNNLGRLEELGDPVADVNAGGRAGIVLVRARN
                     FEIAGNGVRVLQTPMPIDMPGGGIVVSDSSDGVIRGNGVISVADSSGIAADMGAIAVV
                     DDVNVLFGSPA"
BASE COUNT         1307 a         2998 c         2938 g         1389 t
ORIGIN      
        1 agttcggcga ggtcgcgcaa gccgggatcc tcgtgctcgc ccagcagccg cagcgcgcgc
       61 agcctctcct cggaatccat ggcttcgaac aagcccgctt cgaacggcac cgaccggcgc
      121 gcagccgcga caccgtcgag cgcggccgcg agcagtccgg gcaaggacgg atcgagaaac
      181 gtcagatagt ggaggacgaa ccgctcggcc gcgacgtcgc cggcacccgc cgtctgatcg
      241 tcgggtgggg acgggatgac ggcctcggcg agcgcggcga tcgtgcgctc cggtgaggcc
      301 atggccgtgc tacgcgctct gccgcgcgga ggtcaccttg ctgcgctgcg ccagcgccgc
      361 gcggagcggg tcgagcagcg agttctcgtc catctcgaag accggtccga gcgcgtcgag
      421 ctgatccagc gcggcctcca gagccgagcc gagccgctgc acactcgcca gcgcgctgac
      481 gaagttaccg ctgtagtccg cgtccatcgg cgtgccgcag aaggggcagc gaccgacggt
      541 ctctccctcg atcaccagat cggccggaaa gtcgcggcca cagacggtgc agcgtccctt
      601 gatcttcact cgctcctcct cgtcacggtt gccccccggt gcgtcagcgc cttcgccgca
      661 tcggcgagcg cgaacgagtc gatcggccgg accagccatc ggtcggcgcc ggaccagcgg
      721 gccagccagt cgtcgccggc tcgttccagc agcacgatca ccgcaggcgg gtcgatcatc
      781 atcttcagct cgcgcgacag cccgaaggcg ccgaacggct cggcggtctc gtcggtgatc
      841 accagatcga acggatccgt ctcggcgcgc gcgagcgcgt cccggccgtt ggccgcctcg
      901 acgaagtcgt aggcagggcc gagcgcgccg gcgacgaacc gatgccgggc gggatcctcg
      961 gagacggtga gcaccttcat ggcgggcaaa tcctacgggg gggcgctcgg ccgcgcacat
     1021 cgctcgacgc cgcggaccgc gcccctggtc cttgacttcg atgggccctg cgcataggga
     1081 aggtgcggcc ttctttcccg gggaaggaag gatcctcctg cggtaggaaa ggagcctcat
     1141 ggaaaagagc atgggccagc gtctggtggc cgagttcgtc ggcacgctga cgctggtctt
     1201 catcggcgcc ggggcggtcc ttgccgccag catcgggttg ccgggcgcgg gactggtcac
     1261 gtacgcgctc gcgaccggtc tggcgatcgc ggtgatggtc tcggcgctcg gtcacgtgtc
     1321 gggcggccac ttcaacccgg cggtcacgat cggcatctgg atcgcccaga agatcaagtc
     1381 ctcggacgcg atcgcctacg tcgtcgcgca gctcgccgga gccacggcgg gggcggcgct
     1441 gctgcggttc gcgcttcccg agacgctgtg gagggcgggc gcgctcggga cgccgcaggt
     1501 cacgaccggc gtcacgaacg gacaggccgt gttgatcgag gccatcctcg cgttcttcct
     1561 ggtctgggtg gtgttcggat cagccgttga cgccgagggc tcgttcggca agatcgccgg
     1621 gctggggatc gggctcgtct acacgatgga catcatgatg gcgggccccc tcacgggcgg
     1681 cgcgctgaac ccggcccgcc acgtcggacc cgcgctcatg tcgggaacct ggaaaggctg
     1741 gtgggtctac tggatcgggc cggtggccgg tggcatcgtc gcggccgcgc tctacgacgg
     1801 cgtgatcctt cgcaagaagg aagtggggct cgcgcccgac gaggcgccgc acggcttcgg
     1861 cgcgcacggc gacgaggagc ttcccccgct cgcggatccg ccgctcgcgg atccgccggt
     1921 cggcgagtaa ccgttcaaaa accagaaggg ccggcgatct ctcgccggcc cttcgctcgc
     1981 ctctcggctc cgtcgctacg accagcagac gcgctggttg cggctgacgt tccagaggtg
     2041 gtcggtgtcc tgggtcgggt aggcgatccg gaacagcagg cagaagttcc cgttgctctt
     2101 gaactggaac tgataccggc cgttgccgtc caggatgatc tcctggaccc agacccacgt
     2161 gccgctgggc ccgtcgagac gctgcagcca gaccttcttg cccgggtgcg cggggatgac
     2221 cgcgccggac agctgcacgt tcacgcctcg cggcagcgat gacgccgacg agttgaagat
     2281 cacgccgggc cggataccca cccgcagcag cgggctcgac gatggctggt tctcgccgtc
     2341 gccggcgtag ctcgcctgat agtccgacga ggcgctcggg ttcgtctcgg cgaactggaa
     2401 cgtaccgtcc gcggcggtct ggaccgagcc gagctgaatg aagccgctct gccccaccac
     2461 gcgccgccgg atcgagacgg tcttgttggc cagcggcgaa ccgtccccgg tgagcgtgcc
     2521 ggtcatctgc gggtcgatcc cgtacacgcc gaggtcgggc gtgatcgagg cggtgatcgc
     2581 cgtcacgctg cggccccatc gcaccagcac cacgtcggtc gtgtccggct ccacgatgtt
     2641 gcccggcacg ttcgtgccgc cggccggctc gtccggcggc tctccggcgt ccggcccttc
     2701 cgcggtgtcg gccggcatgc cgtggaccca ggcgcgcacc gtcgtcgtgc cgttcgtctc
     2761 gctggtgaag gtggtagacg agccgatcgc tccgggcggg ccggtcaggc tgcacgttcc
     2821 gttgtcgccg gtggtgcact gccggtcggg cgaggcgggc gagtcgcccg agtccgggtc
     2881 gccggccccg atcagctcga agctgacgag cacgccgctc atcggctgca ggttctcgtc
     2941 cttgaccgtt gcggtcagcc cgtagtgcgt gccctgagcg acgttgccgc cgggcggagt
     3001 ctccggcgtg acctcgagct gcgtcgcgac cgaggtgccg ccggaccagg tgacctggcc
     3061 gcgatcggtg agcgcgttgc cgccgtcctc gccgggggcg tccggctcgg tcgcgcacgc
     3121 accgccgtcg ccgacggcgc cgccgggcgc gtagaagtcc gcggtgcccg tgtcctgcca
     3181 ggcgcagaac cggtcgacgc cgttgtttcc gttggacgcg taggtaattc cgcaggtgcc
     3241 gttcgcgagc gtggtgcatt ccttgttcac ggtcgatccg gtctgcgcgt tcggtccgcc
     3301 gagcacgtac aggtcgatgt tgacgttgcc gatcccgtcg ccgaactggt cgatcgcctt
     3361 gacgttgacg gtgtggttcg gctcgccggg aagcacgttc gcgtcgtcct cgttgaggat
     3421 gtcgatcgtg gtgaccacgc gcgtctgcca ggtcacctgc atcacgtcgg tgccgttgtc
     3481 gttctcggcg gcgtccaccg gctcgttgca gttgccgccg tcgccggccg tagcgtcgtt
     3541 gacctcgtcg tcgttgtcgc cgtcgatcca ggcgcagatg agcgccgttc ccgcctgcag
     3601 cgtgccggtc tgcagcggca cgtcgaacgt gcaggtcccg gccgcggtgg tcacgcagcc
     3661 gtagtccggc ggagccacct tgccggcgtc ggcgtcgttg acgccggcgg tcaggttctc
     3721 ggcgtcgatg gtgacgttgg cgacgccggt gcccgagacg gtgaccttgc aggtataggt
     3781 gacgtgtgtt gcggcctcgc tgccggtggt gggcacgacc tcgatgtcgt cgccgttgcc
     3841 ggcgggcgcc tcgtcgtcgc agtccagcac ctgcgcgccc ccgcctccgc caccggttcc
     3901 caccacgacg ctcaccacgt cggtgcgatc cggctcggca acgtcgccgg gcaccagcag
     3961 ctcgttgatg ccctcgccgt ggtcgggctc gctctgcggc gcggcaccgc tgtgatggac
     4021 ccaagcccgg atgctgtcca cgttggcgaa cgcgggcgtg tactgggtga cgcaggacga
     4081 catcgtgatc gcgacggtgc acgacatgtc gggagtctca ggcgtgtatc cgtccgcgtc
     4141 gttcgggccg ccggtgagct cgaagtacac gaccgtcgac gtcgtcgccg gcgcgcccag
     4201 cgtggccgtc agctgatacg gcgagccggt ggctgccgct ccggtctcgg gctccacgtc
     4261 cagggtctgg agggcgtggg cggcaaagcc gggttgcgga accgaggcaa gcaccaccgc
     4321 cgccaacaga accatggcgg ccggtaaggt ccgccgccgg cgtgaatttg agcgagcctt
     4381 caaaactgca cctccgtgtc gatcaacgac gccacatacc gcgaactgtg tcccacccaa
     4441 cgatggggcg caagcaacgc agccgtattt gtccaggatg aatcgtccgt tatcggatca
     4501 aaccgggacc ccttgggttg gcaacgcgcc cggacgtgac cgccgaaacc cggttgtcaa
     4561 cgtttcgcgt catcaaagcg tataacgagc gtgaacgcca tcgttgatca accctcgagg
     4621 agcggcatct ccgtcttcgg tccgtcgtcg gagcggctcg accgcctcgg cgagtggttc
     4681 gccgccgagt acgagccgct tcttcgcttc gcctatttcg tcaccggcga ccacgaggcc
     4741 gctcgcgaca ccgtccagga cgcgttcatc aagatctaca aggcgggcgg acgcgtcgag
     4801 cacgaagggt tccccgccta cgcgcgccgc acggtcgcga acctcggccg gtcacgcttc
     4861 cggcggcagc ggagcgagcg cgccgcgatc gccggcctgc cgcgcgtcgc cgaaggcgtg
     4921 cgcgattccg acccggtcga gcgcgacgag gtctgggccg cgatcctgcg gctgtccaag
     4981 cagcagcggg ccgtcgtcgc tctccgcttc tacgagcaca tgaccgaaag ccagatctcc
     5041 gcgacgctgg gcgtgagcgc cggcaccgtc aaaaagcagc tgtcgcgcgc gctggaccgt
     5101 ttgagcatcg aattggaagg gaggccgcga tgaccgatcg tcgtcgcgac atcgaatctc
     5161 gagtcgagga ggctctcatg cgccgctcca ccgatgtcga accgcagccc ggcgccgact
     5221 gggacggcgt ccgcggcggc gtgcaccgcg cgcagcgcgt ccgcgtcgcc gtctcgaccg
     5281 tcgccgcgct cgcgctgatc ggcgccggcg tgttcgtggt gcccaagctg atgggccgcg
     5341 attccgggat cacgcccgtg aagcccggcc ccagcgtgac ctcgcccacg ccgacggcga
     5401 ctcctcccgc cgtgccggcc gattggcagg tgcttcgcta cgatccgcag ggctactggc
     5461 tcaccgttcc ggcctcgtgg aggaccggct ggttcgaagg ccacgcggag taccggccga
     5521 agggactgcc gtccgcggta caaggcgaag acacattctt cgtcgagaca ttctcatccg
     5581 ggggcgtggc cgagccggag ggcgatccgg aagcgctcac gatcaacggt gcccccgcca
     5641 aacggtggga gacaccgtcc gagagcggtg cctacacggt caccttcgac atcacgttgc
     5701 cgtgctacgc cggcccgggc tccagcgcac acatcgactg cgcgctggtc ccgctccgcg
     5761 tcgtcgtcta cgcctctacg gatgcgctct ggaaagagca cggcaagacc ggcgaggaga
     5821 tcgcccggtc gattaccgcc gccgaacgca cgatgccggt ccaggacggt cagctctatc
     5881 gaaccttcca aggcggatcg acggtcgcat gggacgccaa gacctgggcc gtggtcgagt
     5941 tcatggagtc gcgcatcttc ggcgacaccg agcgcggcga ccgcttcttg accgcaaacg
     6001 ccaagaagca atacgaaacc ggcgaaggcg gcctggaccg gtacggaccc gattcgaagt
     6061 tccgtttggt gacctacacc atcgagcgcc gcgaaggggc cgacgcgaac tccgacgagt
     6121 tcaccgttcg catcaccgag caacggctcg gagccgagcc cgtcaacgag atcatcgaaa
     6181 cgctcggcgt cggaccgacg gaatcgaatc acgtgttcgt cccgtatcag atccgcttcg
     6241 cgacccgcag cgaagcgtag aaccgtctag agccagcggc ggcgcttgaa gaacacatag
     6301 agcgccgccg ctgcggcgcc catcgccgag acggcgacga agaatccggt cgtgcttccc
     6361 tcgcgcggga acagggcgaa gttcatgccg tagacgcttg cgatcaacgt ggggatcgcg
     6421 atgatcgccg cccagccggt cagcttcttg gtcacctcgt tgagcgcgtt ggcctgctcg
     6481 gcccggcgga actccatcat cgcgttcact aggtcctcga cgttgcggat ctggtcaccg
     6541 atccgaagcg tgtgatccag cacgtcccgg aaataggcgg ccgtcgtctc gctggcccct
     6601 cccccggctc gcgcggcctc gacgaagccg gccagcgcgc ggctgcccgg catgacgtac
     6661 cgccgcatgc gcgcgatccg ctgcttgatc gtgtagacct gctggccgga tccaccgacg
     6721 ggattggcca gcgtgccttc ttccagctcc tcgacttcgg cctcgatcgc gtccgcgatc
     6781 gcctggtact cgtccacgac cgcgtccacc agcgcgtgca tgatccacga gggcccccgg
     6841 ccgggctcgc gctcggtgcg ccgcgttcgg gcgaccgcgc gctcgacgag ccggtcggcc
     6901 ccgtcgtgga tggtgagcac gtagtccttg ccgacgaagc acgcgatctg gcgcgcctcg
     6961 agctgaccgg cgacttcgtc gagctgatgc accacgacga acagatgatt atcgtaggac
     7021 tcgactttgg ggcgctggcg ggcgttgagc gcatcctcca cggcgagcga atggaggccg
     7081 aactcctcgg cgatctgcgc cgcgtcctcc gatgtaagcg aaccgatatc ggcgtaggcc
     7141 cacagcagct cgcccggctc ggcgatggcc tcgctgatcc ggcggagatc gcccagctcc
     7201 gaccatccct cgcccggcct gtgcgtgaac gccttcagca tgcccgaacc ctacccatga
     7261 ttcgcttgct cacccggacc gaggaaatcg gacggcgtgg gagggatgag gactaattgt
     7321 gcagaactag caaggcagag cattatcagg cctaattcgg acttttcagt acttgcggta
     7381 accaggaccg cgcgccgttc gaaacgacag ggggagtttc cggtgtcacg aacacgaaag
     7441 cattccgttc cggttcgctt gctgctcgcg ctcgcgatcg cagcgggcat ggccgcggct
     7501 cccggcgggg cggtcgtgcc gctccggctc gggctggtgc tgccggtcga ctgcaacacc
     7561 tcggtggacg acatcgcggc cgtgcaggat gcggtcgacg ccgccggaga cggcgacacc
     7621 atccagttgt ccggcgtctg cgacttcagc ggagccaagc cgcacggagg cgacatcgcc
     7681 tcgatcaccg cgaccgcggt tctgatccgc gcgggctcgc ccttctccgc aggcctgacg
     7741 atcgagtcgg ccgaggacgc ccgggcgacc gtcctggggt cgggaacgca gaccgcgttc
     7801 atggtcgctc ccggcaacga caacgtgacc atccgcggac tgaacctgct gggcttcgca
     7861 cgtccgatcg tggtgtggtc cgcgtccaac accaccatcg gcgatgccgc ggcaacctcg
     7921 gctccctcgg cggacggcaa ccggatcgcg ggcgacgcga tgaactcggc cgtgctcgcc
     7981 atcggaaaca acaaggacgc ctcgggcgcc acgcccctca ccgccgtttc ctacggctcg
     8041 ggctcggccc gatcggcgct gcttgccaac aacggccggc tgaccaacct tcgggtgctc
     8101 ggcaactaca tcacctacgg cgccgtggac gcgcccgacg ggcagacccg cgacgtggtg
     8161 gcaatcgacg tgcgccagcg caaccagggc aacgtcgacg gcgttgagat cgcccacaac
     8221 gcggtgggct tcctcaccgc aagcttcccg tcgttcaaca tcaacgccgt tcgcgtgcac
     8281 gcgcactccg gggacccgaa ctatcacctg agcaacgtct cgatccgcaa caacaacctc
     8341 ggccgcctcg aggagctcgg cgacccggtc gccgacgtca acgcaggcgg acgcgccggc
     8401 atcgtgctgg tacgcgcgcg caacttcgag atcgccggca acggcgtgcg ggttctccaa
     8461 acgccgatgc cgatcgacat gccgggcggc ggcattgtgg tgtcggactc cagcgacggc
     8521 gtgatccggg gcaacggggt catctcggtg gccgactcgt ccgggatcgc ggccgacatg
     8581 ggcgccatcg ccgtcgtgga cgacgtcaac gtgctgttcg gctcgccggc gg
//
LOCUS       DATLFO010000161        10979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_122226, whole genome shotgun sequence.
ACCESSION   DATLFO010000161 DATLFO010000000
VERSION     DATLFO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122226"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VM841_06175"
     CDS             complement(<1..697)
                     /locus_tag="VM841_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVL89806.1"
                     /translation="MRAPTRPVALLAALLIGLTACIGSDDPPASDPIPSSLASLITPG
                     MPRWRVLAEAPEPRQEVAAARLGGKVYVVGGLTASGATTRVGIYDVAGDSWTDGPPLP
                     VALHHAMAAVFGGAVHVIGGFDPSSRPVASVFRLEGSAWKEAAPLRRPRAGGIAVVEK
                     ERLFVFGGQGAGELVAPVEELVDGAWVDRAPIPTPRHHVAAASDGEWIYVSGGRRPAS
                     GGPDENVAAFERFS"
     gene            complement(725..1975)
                     /gene="rpoD"
                     /locus_tag="VM841_06180"
     CDS             complement(725..1975)
                     /gene="rpoD"
                     /locus_tag="VM841_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HVL89807.1"
                     /translation="MGLPSETRPDEVSQLVAKARARGGFVTQDELDAFLAAQDLTPEQ
                     IDSVQTALTDEGVEVVEVPSPPDLEEAAEIEAEAREIEAALAKSQTSDPVRMYLREIG
                     RVPLLTGEQEVYLAMRIEAGLEAGPFLSGERRGPSPEELSALAKKADVATMLPRQGAT
                     PDVAKEILRRIELEGSLAKKKLIESNLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIRA
                     VEKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQMMQD
                     LGREATPEELAEQMELPVEKVLDIQKVALEPVSLETPIGEEEDSHLGDFIEDEGAIVP
                     ADAASFILLQEQLEGVLGSLSDREKKVIQMRFGLFDGHQRTLEEVGREFGVTRERIRQ
                     IESKTLSKLRHPSRSQKLRDYLDE"
     gene            complement(1980..3788)
                     /gene="dnaG"
                     /locus_tag="VM841_06185"
     CDS             complement(1980..3788)
                     /gene="dnaG"
                     /locus_tag="VM841_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HVL89808.1"
                     /translation="MSGGTGKGRIADADLDLLRDKASIVEVAADYMKVRQAGRIFKAL
                     CPFHSEKTPSFSIDPAKGVFYCFGCQKGGDVITLVRELESLDFVEAVEHLARKTGITL
                     HYEQLSAAERDRSKTRNRLIDAHREAVAYYHALLKSSAGAKKARQYLASRKLKPETLE
                     RFQVGWAEANWDGLTKHLKTRGFRDDELTTGGLSLRTERGGLIDRFRGRVMFPIFDVT
                     GEPRAFGARKLLDDDDGPKYLNSAESPIYKKGTILYALNWGKAEIVKADVAIVVEGYT
                     DVIALHQEGIPLAVATCGTALGVEHFQSLSRFTKNVVIALDGDAAGQSAAERAVERAY
                     LDAQQRDMSLRVLTLPAGSDPAEHVVARGGEEFRRTLQDSVPVVEFRLRARLQGLDLS
                     EPEGRSRGLRACLPVLAQVADQVVLRDYTRWVADRCGLDYDIVFLEVRKALRGSSTPG
                     PAGIKQASSQVTKEREALKLALQFPDLVEEHLERLDPDDFQVPSHRSAWEQIRRGDTD
                     PSHAPEDGVRALMTRLAVEPVEGVPDGEPGDRLIDEIFTRLEEFAVTRQINEVRSRLE
                     KLNPVNDPPSYDALFKELVALEAARRRMTIDEPAGA"
     gene            complement(3785..4774)
                     /locus_tag="VM841_06190"
     CDS             complement(3785..4774)
                     /locus_tag="VM841_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HVL89809.1"
                     /translation="MPTISEETERIEREILSPHAQLASAGKGRERPEEPDPLRTCYQR
                     DRDRIVHSKAFRRLKHKTQVFFSPQGDHYRVRLTHTLEVTQVARTIARALRLNEDLTE
                     AIGLGHDLGHTPFGHIGEGVLSRLLGRPFKHSEQSLRIVDLLEDGGRGLNLTWEVRDG
                     IKAHTWSQPEPSTLEATCVRFADRIAYLNHDVDDAIRDGIITIDDVPDGVLDVLGQTH
                     SDRINTLVTSVVEASHGKPEVRMTEAAAAAMQTLRDFLFERVYVIPETAEQRARYEGI
                     VEDLFRYYLEHQDEISDEYHAVEADPFTRAADFVAGMTDRYALTRHAEIGGAE"
     gene            complement(4927..5220)
                     /locus_tag="VM841_06195"
     CDS             complement(4927..5220)
                     /locus_tag="VM841_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89810.1"
                     /translation="MEKLRLIAGAVALAVAGLLAVVVVVRDAVNPATVWTIAGGAIAG
                     VMLLRLRSRASDIAASTLLLLAAAPAMIGGMGWLLVPSMMLAWGVALAPRDPA"
     gene            complement(5354..6628)
                     /locus_tag="VM841_06200"
     CDS             complement(5354..6628)
                     /locus_tag="VM841_06200"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL89811.1"
                     /translation="MTSAATPERRFAAVRALMRNADFRLLCSSQLLAGLGEWLATMAL
                     VALVWERTHSAVAGGLVLVVRILPAAVLGTVLGSVVDRFDRKKVMVACNGGRAIVYGV
                     LPFIAGTAPVLALALLAEIGAIAYATARDATLPRLCAPEYLPAANAISMASAFGMMPV
                     GSGLFALFGLAGRHAVTLALAAAGLACAIATLIVGRVNAHAAAGGDAIQRPEHVSFLD
                     GLRALREVFREDAILRRVGLGAAIAATGGGAVITLGLAYVRGTLHAGSTAYSILLVSF
                     CAGAIAGVVSLQRFRGVLHHVFHGGVGVMGLILVLMALFPSTGVGVGMAFLFGGAFVA
                     TFLGGVTILQDRVRDSLRGRAFGLAHSVLRAGAVLMGLVSAWAAHAIGERQRDFGMVN
                     MDGNQLVLAVAGTLLFAAAATLLRPARVRARG"
     gene            6920..7534
                     /locus_tag="VM841_06205"
     CDS             6920..7534
                     /locus_tag="VM841_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89812.1"
                     /translation="MRKIWLGFGIAITALAGSILPSGAGQHFGVLGSDGGTFVTGNAF
                     GPEGARGDELSASGVFVRGALVDVRDGSATRSSLYACVSVKTAQGRDHGCVSGDGFPQ
                     MSDPMLSSAELFMTIPSSLHQGGFIEIELLWSADSPFEPQVSFSLPDGQISASVAAGI
                     ARRAIVTGTIRSSAVGGGPINADGFLTRGTSVRACSSDTGQAPC"
     gene            complement(7601..10387)
                     /gene="ppdK"
                     /locus_tag="VM841_06210"
     CDS             complement(7601..10387)
                     /gene="ppdK"
                     /locus_tag="VM841_06210"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HVL89813.1"
                     /translation="MPSARRSKPKTTATTKRATKAAKARSRPSTPASVKRPAAKKTSA
                     PAKRSGKFVSSFSEGSKEMKDRLGGKGANLAEMTGMGLPVPPGFTITTDACREYMHTG
                     TFPPNLMDEVERHLRALEKAMGRTLGDPDDPLLVSVRSGAKFSMPGMMDTVLNLGLND
                     RSVNGLARVSDNPRFAWDAYRRFIQMFGKVVMGADGERFEHALDEAKKRKGVAVDTDL
                     DEQDLRELVDEFKVIVKEETGQQFPNDPRKQLELAVKAVFDSWNGDRAKLYRRQEKIS
                     DELGTAVNVQAMVFGNLGADSGTGVAFTRDPATGDSGQPYGDYLENAQGEDVVAGIRN
                     TLPISELERINGKAYKQLLQIMNKLERHYRDMCDIEFTIERKKLWMLQTRVGKRTAAA
                     AVKLAVQMRKEKLIDEKEAVRRVTPEQIDMLLHPQVDEERFEGSVLATGLNASPGAAV
                     GVAVFDADRAEERAATEKVILVRRETNPDDLNGMIAAQGILTSRGGKTSHAAVVARGM
                     GKPCVCGADALDVNLKANEFRVGDTVVREGELISIDGSTGKVFLGEVPLKQATLSGDF
                     ATLLGWADKHRRMKVRANADTPDDAAKAREFGAEGIGLARTEHMFLGDRRPLVERLIL
                     ASNEAEEQAALDALLPLQRADFKGILAAMDRLPVTIRLLDPPLHEFLPDRVELAVEIA
                     KLEAKGKDVPGEKRTLAHAVERLHEANPMLGMRGVRLGVMKPGLYTMQVRAIMEAACD
                     LKEEGRNPVVEIMIPLVSIGEELRRSQRDADRVAKEVLKARGVRVKYLIGTMIELPRA
                     ALVAGEIAETAEFFSFGTNDLTQTTFGFSRDDVARILDVYVTHGILKVDPFTTIDREG
                     VGALVRTATEQGRAARAGLKVGICGEHGGDPDSIAFCDEIGLDYVSCSPFRVPVARLA
                     AAHATLGAGDAASK"
     gene            10508..>10979
                     /locus_tag="VM841_06215"
     CDS             10508..>10979
                     /locus_tag="VM841_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89814.1"
                     /translation="MSPRVDRVSLRTPRLTLMPFAEAHAAAVWAAIEPSLEDLRPWLV
                     WTRWTSERATAEFVESCPGAWRNGSGFSFAVLEGEDLIGSCSLAIHNPNLTQGEIGYW
                     LRSDRAGNGYTTEAARAVRDFGFDATGCHRLNLRAGVGNVASQRVAEKIGFTREG"
BASE COUNT         1735 a         3704 c         3730 g         1810 t
ORIGIN      
        1 cgctgaagcg ttcgaacgcg gcgacgttct cgtcggggcc gccgctcgcc ggccgccgtc
       61 cgccggagac gtagatccat tcgccgtcgc tggccgcggc gacatgatgc cggggcgtgg
      121 ggatcggcgc gcgatccacc cacgcaccgt cgacgagctc ttcgacgggc gcgaccagct
      181 cgccggcgcc ttggccgccg aagacgaaga gccgttcctt ctcgaccacg gcgatgccgc
      241 cggcacgcgg ccgccgcagc ggcgcggctt ccttccaggc ggatccctcg aggcggaaaa
      301 cgctcgcgac cggacggctc gacgggtcga agccgccgat gacgtggacg gcgccgccga
      361 agaccgcggc catcgcgtgg tgcagcgcaa ccggcagcgg cgggccgtcg gtccaggagt
      421 cgccggccac gtcgtagatg cccacgcgcg tcgtcgcacc cgaggcggtc aaccctccca
      481 cgacgtagac cttgccgccg agtcgcgcgg cagccacctc ttgccggggc tcgggcgcct
      541 cggcgagcac gcgccagcgc ggcatgccgg gcgtgatcag cgacgcgagc gagctcggga
      601 tcggatcgga cgccggcgga tcgtcggagc cgatgcaggc cgtcaggccg atcagcagcg
      661 cggcgagcag agcgaccggc cgggtggggg cacgcatggc gcccagtcta tgcaacgggg
      721 cgcgctactc gtcgaggtag tcgcgcagct tctgcgagcg ggacgggtgc cggagcttgg
      781 atagcgtctt ggactcgatc tggcggatcc gctcccgggt caccccgaac tcgcgtccga
      841 cctcttcgag cgtgcgctgg tggccgtcga agaggccgaa gcgcatctgg atgaccttct
      901 tctcgcggtc cgacagcgag ccgagcacgc cttcgagctg ctcttgcagc aagatgaacg
      961 acgcggcgtc cgcggggacg atcgcgccct cgtcctcgat gaagtcaccg agatggctgt
     1021 cttcctcttc gccgatcggc gtctcgagcg agaccggctc gagcgccacc ttttggatgt
     1081 cgagcacctt ctcgaccgga agctccatct gttcggccag ctcttcgggt gtggcctcgc
     1141 ggccgaggtc ctgcatcatc tggcgctgga tacggatcag cttgttgatc gtctcgacca
     1201 tgtgaaccgg tatgcggatc gtgcgcgcct ggtccgcgat cgcgcgcgtg atcgcctgac
     1261 ggatccacca cgtcgcgtac gtcgagaact tgaagccctt cgagtagtcg aacttctcga
     1321 ccgcacggat caaacccaga ttgccctctt ggatcaggtc gaggaactgc atgccacgtc
     1381 cgacgtagcg cttggcgatc gacacgacca gacgaaggtt cgactcgatc agcttcttct
     1441 tggcgaggct tccttcgagc tcgatgcggc gaaggatctc cttcgcgacg tcgggcgtgg
     1501 cgccctgccg cggcagcatc gtggcgacgt ccgctttctt cgcgagcgcg gacagctctt
     1561 ccgggctcgg gccgcgacgc tcgcccgaga gaaacgggcc ggcttccagg ccggcctcga
     1621 tgcgcatcgc gagatagacc tcttgctcgc cggtcagcag cgggacccgg ccgatctccc
     1681 gcaggtacat gcgcacggga tccgaggtct gcgacttggc gagcgcggct tcgatctcgc
     1741 gggcctcggc ctcgatctcg gcggcctctt ctagatccgg cggggaggga acctcgacga
     1801 cctcgacgcc ttcgtcggtg agcgccgtct gaaccgagtc gatctgctcg ggcgtcaggt
     1861 cctgggcggc gaggaacgcg tcgagctcgt cctgggtgac gaatccgccg cgagcccgcg
     1921 ccttcgcgac gagctgggac acctcgtcgg gtcgcgtctc gctgggaagg cccacggtct
     1981 cacgctcctg ctggctcgtc gatcgtcatc cgccgtcgag ccgcttcgag cgcgacgagc
     2041 tctttgaaaa gcgcgtcgta gctcggcgga tcgttgacgg ggtttagctt ttccagtcgg
     2101 cttcgtacct cgttgatttg ccgtgtgact gcgaattcct ccaaccgggt gaaaatttcg
     2161 tcgatcagcc tatcgcccgg ctcgccatcc ggcaccccct cgacgggctc gaccgcgaga
     2221 cgcgtcatca gcgccctcac cccatcttcg ggggcgtgcg agggatcggt gtcgccgcgg
     2281 cggatctgct cccaggcgga tcgatggctg gggacctgaa aatcgtcggg gtcgagccgt
     2341 tcgaggtgct cttcgaccag atcggggaac tggagggcga gcttgagcgc ctcgcgctcc
     2401 ttcgtgacct gagaagaggc ttgctttatg cccgccggtc cgggcgtgga ggaaccccgg
     2461 agcgctttgc ggacttccag gaacacgatg tcgtagtcga gtccgcatcg gtcggcgacc
     2521 catcgggtgt agtcgcgaag gacgacttga tctgcaactt gtgcaaggac cggcaggcac
     2581 gcgcggagcc cgcgcgaacg gccctcgggt tcggagaggt ccaacccttg aagccgcgcg
     2641 cggagccgga attccacaac ggggacactg tcctgcaatg ttctacggaa ctcctcccct
     2701 ccccttgcaa caacgtgttc cgctgggtcg cttcctgccg gaagtgtgag aactctgagg
     2761 ctcatatcgc gctgctgagc gtccaagtaa gcgcgttcga cggcgcgctc ggccgccgac
     2821 tggcccgcgg cgtcgccgtc gagcgcgatc acgacgttct tggtaaagcg agaaagcgat
     2881 tgaaagtgtt cgaccccgag cgcggtgccg cacgtcgcca ccgcgagcgg aatgccttcc
     2941 tgatggagcg cgatcacgtc ggtgtagcct tcgacgacga tcgcgacatc ggccttgacg
     3001 atctcggcct tgccccaatt gagggcgtac agaatggttc ccttcttgta gatcggcgac
     3061 tccgcggagt tcagatactt cgggccgtcg tcgtcatcga gtagctttcg cgcgccgaac
     3121 gcacgtggtt caccggtcac gtcgaagatc ggaaacatca cgcggccgcg gaatcgatcg
     3181 atcaatccgc cacgctccgt gcgcagcgac agcccgccag tggtcagctc gtcgtcgcga
     3241 aagccgcgtg ttttcaaatg tttcgttagg ccgtcccagt tcgcttcggc ccaaccaacc
     3301 tgaaatctct cgagcgtttc gggtttgagc tttcgcgatg caaggtactg ccgcgccttc
     3361 ttggcgccgg cggacgactt cagcagcgcg tggtagtacg cgaccgcctc acggtgcgcg
     3421 tcgatcagac ggttgcgcgt cttggatcgg tcacgctcag cggccgagag ctgctcgtaa
     3481 tgaagcgtga tcccggtctt gcgtgccaag tgttcgaccg cctcgacgaa gtcgagagac
     3541 tcgagctcgc gcacgagcgt gataacgtcg ccgcccttct ggcagccgaa gcagtagaac
     3601 acgcctttcg cgggatcgat cgagaagctc ggggtcttct cggaatggaa cgggcacagc
     3661 gccttgaaga tccggccggc ctgccgcacc ttcatatagt cggccgcgac ctccacgatc
     3721 gaggccttgt ctcgcagcag gtccaggtcg gcgtcggcga ttcgtccttt gccggttccg
     3781 ccgctcattc cgctcccccg atctcggcgt gccgggtgag cgcgtatcgg tcggtcatgc
     3841 ccgcgacgaa gtcggcggcg cgggtgaacg gatcggcctc gaccgcgtgg tactcgtcgc
     3901 tgatctcgtc ctgatgctcg aggtagtagc ggaacaggtc ctcgacgatg ccttcgtagc
     3961 gcgcgcgctg ttcggcggtc tcggggatga cgtagacgcg ctcgaacaga aagtcgcgga
     4021 gcgtctgcat cgccgcggcc gcggcctcgg tcatgcgcac ctccggcttg ccgtgcgagg
     4081 cttcgaccac cgacgtcacg agcgtgttga tccggtccga gtgcgtctgg cccagcacgt
     4141 cgaggacgcc gtccgggacg tcgtcgatag tgatgatgcc gtcgcggatc gcatcgtcga
     4201 catcgtggtt gagataggcg atgcgatcgg cgaaccggac acaggtagcc tcgagcgtgg
     4261 acggctcggg ctgggaccac gtatgggcct tgatgccgtc gcgaacctcc caggtcaggt
     4321 tcaggccgcg gccgccgtcc tcgagaagat ccacgatgcg cagggactgc tccgaatgct
     4381 tgaacggccg gccgagcagc cgcgagagca caccctcgcc gatatggccg aacggcgtgt
     4441 gccccaggtc atgaccgaga ccgatcgcct cagtgagatc ctcgttcaga cgcagcgccc
     4501 gcgcgatcgt gcgtgcgacc tgggtcacct cgagcgtgtg agtcaggcga acgcggtagt
     4561 gatcgccctg cggcgaaaag aacacctgcg tcttgtgctt gagccgacgg aaggccttgg
     4621 agtggacgat ccggtcgcga tcgcgctgat agcaggtgcg cagcggatcc ggctcctcgg
     4681 gacgctctct ccctttgccg gccgacgcca gctgcgcgtg cggcgagaga atctctcgct
     4741 cgatgcgctc ggtctcttca ctgatcgtag gcaagattct ccgcacccag cgtactcgct
     4801 cgcaccgaca tgatcgctcg aaggctcagg gatgctagaa acgccgccac agagagtgca
     4861 agaacgggaa tttcgaccgt gggttgacga gcgggacggg gcgtgcagtg cggccgaagc
     4921 ggccgttcag gcggggtccc gcggcgcaag ggcgacgccc cacgcgagca tcatcgacgg
     4981 gaccaatagc cagcccatcc cgccgatcat cgccggcgcc gcggccagca gcagcagcgt
     5041 cgatgccgcg atatcggacg cgcgcgaccg gagccgaagc agcatgacgc ccgcgatcgc
     5101 tccgcccgcg atcgtccaca ccgtcgccgg gttcacggca tcccgcacga ccacgaccac
     5161 cgcaaggagc cccgcgaccg caagcgccac cgcacccgcg atcaaccgaa gcttctccat
     5221 gcggtcatct taaaccccga gggttgacag catgtccgct cgtccatggc ggcggggccg
     5281 ggaggaaaga ctctccgaca ccgccggccc ggcagacgga ccggcgtcgg ggaggcggga
     5341 cggcggaagg cggctagccg cgagcgcgca cgcgcgccgg gcgaagcagc gtggcggcgg
     5401 ccgcaaacag cagggtgccc gcgacggcaa ggacgagctg gttgccgtcc atgttgacca
     5461 tgccgaagtc gcgctggcgc tcgccgatgg cgtgggccgc ccacgccgac accaggccca
     5521 tcagcacggc tccggcgcga aggaccgaat gcgccaggcc gaacgcgcgg ccgcgaagcg
     5581 agtcgcggac gcggtcctgc aggatcgtca cgccgcccag gaacgtggcg acgaacgcgc
     5641 cgccgaacag gaaagccatt ccgacaccga cgcccgtgga cgggaacagc gccatcagca
     5701 ccaagatcaa gcccatcacg ccgacgccgc cgtgaaacac gtggtgcagg acgccgcgga
     5761 accgctgcag cgacaccacg cccgcgatcg cgccggcaca gaagctgacc agaaggatcg
     5821 agtacgcggt cgagccggcg tgcagcgtgc cgcgaacata ggccagaccg agcgtgatca
     5881 ccgcaccgcc gccggttgcc gcgatggcgg cgcccaaacc gacgcggcga aggatcgcgt
     5941 cctcacggaa gacctcacgc aacgcgcgca agccatcgag gaacgacacg tgctcgggcc
     6001 gctggatcgc atcgccgccc gcggcggcat gggcgttgac gcggccgacg atcaacgtcg
     6061 cgatcgcgca cgccaaaccg gcggcagcga gcgcgagcgt gacggcgtgc cgcccggcaa
     6121 gaccgaacag cgcgaacaaa ccggagccga ccggcatcat gccgaacgcc gacgccatcg
     6181 agatcgcgtt cgcggcggga agatactcgg gagcgcagag gcgcggaagc gtcgcgtcac
     6241 gcgcggtcgc gtaggcgatc gcgccgatct cggccagcag cgccagcgcc agcaccggcg
     6301 cggtacccgc gatgaacgga agcacgccgt agacgatcgc gcggcccccg ttgcaggcga
     6361 ccatgacctt tttgcggtcg aagcgatcca cgaccgagcc gagcacggtg ccgagcacgg
     6421 ccgcgggaag gatgcgaacg acgaggacca agccacccgc gaccgcggag tgggttcgct
     6481 cccagaccag agcgaccagg gccatcgtgg cgagccattc gccgagcccc gcgagaagct
     6541 ggctggagca taggaggcgg aaatctgcgt tgcgcatcag ggcgcggacg gcggcgaacc
     6601 ggcgttcggg ggtggcggcg ctggtcacgt gatgcctcct gcgggcaggt ctccctcatg
     6661 aatcggctcg cccgaaccgt tcttgagcct caacgacgat ggtgccgcgc cgttcggctt
     6721 gagtggcggg agaatccgta ccctgcggac cgagcgcatg gaccaggccg cggggaagag
     6781 cggatcgcat tgtcggcgac cccgccgggc ctcgcaactg ggactttcat ccacctcgag
     6841 tatgcaaaag gtcatagaag ggacccgccg gacccgcgca gaatcattga ggcagcaagc
     6901 tctggaattg gggggacacg tgcgaaagat ttggctgggg ttcgggatcg cgatcacggc
     6961 gcttgcgggc tccatcctgc cgtccggcgc cggacagcat ttcggagttc tcggaagcga
     7021 cggcggcaca ttcgtaaccg ggaacgcatt cgggccggaa ggggctcgcg gcgacgagct
     7081 gtccgcttcg ggcgtcttcg ttcgtggtgc cctcgttgac gtacgagacg ggtcagcgac
     7141 gcggtcgagc ctctacgcgt gcgtttcggt caagaccgcg caaggccgcg accacggatg
     7201 cgtgagcggc gacggcttcc cgcagatgtc cgatccgatg ctgtcgtccg ccgagctgtt
     7261 catgaccatc ccgtcgtcgc tgcatcaggg cggcttcatc gagatcgagc tcctgtggtc
     7321 ggcggactcg ccgttcgagc cgcaggtctc gttctcgctt ccggacggcc agatctccgc
     7381 ctcggtcgcg gccggcatcg cgcggcgcgc catcgtcacg ggaacgatcc gctcctcggc
     7441 cgtgggcggc ggtccgatca acgcggacgg cttcctgacg cgcggcacgt cggtgcgggc
     7501 atgctcgtcg gacaccggcc aggcaccctg ctagacgaac tcgacagcca agagccggcc
     7561 cggtcgaagg gccggctctc ctctttcctg cttctctttc ctacttcgac gcggcgtcgc
     7621 ccgcgccgag cgttgcgtgc gcggcggcga gccgcgcgac cggaacgcga aacggcgagc
     7681 acgacacgta gtcgaggccg atctcgtcgc agaacgcgat cgagtcggga tcgcctccgt
     7741 gctcgccgca gatgccgacc ttcaggcccg cgcgcgccgc gcgtccctgc tcggtcgcgg
     7801 tgcgcaccaa cgcgccgaca ccctcgcggt cgatcgtggt gaagggatcg accttgagga
     7861 tgccgtgggt gacgtacacg tccaggatgc gcgcgacgtc gtcgcggctg aacccgaacg
     7921 tggtctgcgt caggtcgttg gtgccgaaac tgaagaactc ggcggtttcg gcgatctcac
     7981 ccgcgaccag cgcggcgcgc ggaagctcga tcatggtgcc gatcaggtac ttgacccgga
     8041 cgccgcgagc cttgagcacc tccttggcga cccggtccgc gtcccgctgg ctgcggcgaa
     8101 gctcctcgcc gatgctcacg agcgggatca tgatctccac gaccgggttg cggccctcct
     8161 ccttcaggtc gcacgcggct tccatgatcg cgcgcacctg catggtgtag agcccgggct
     8221 tcatcacgcc gagccggacg ccccgcatgc cgagcatcgg gttggcctcg tgcaaccgct
     8281 cgacggcgtg agccagcgtg cgcttctcgc cgggaacatc cttccccttg gcttcgagct
     8341 tggcgatctc gacggcgagc tcgacccggt cgggcaggaa ctcgtggaga ggcggatcga
     8401 gcaggcggat cgtgaccggc aggcggtcca tcgccgcgag gatgcctttg aagtcggcgc
     8461 gctgcagcgg cagcagcgcg tcgagcgccg cctgctcctc ggcctcgttg ctcgccagaa
     8521 tcagccgctc gaccagcggg cggcggtcgc cgaggaacat gtgctcggtg cgggccaggc
     8581 cgatcccttc cgccccgaac tcgcgcgcct tcgcggcgtc gtcgggcgta tcggcgtttg
     8641 cgcgaacctt catgcgccgg tgcttgtcgg cccagccgag cagcgtcgcg aagtcgccgg
     8701 acagcgtcgc ctgcttgagc ggcacctcgc cgaggaacac cttgccggtg ctgccgtcga
     8761 tcgagatcag ctcgccctcg cgaacgacgg tgtcgccgac gcggaactcg ttggctttca
     8821 ggttcacgtc gagcgcgtcg gcgccgcaca cgcacggctt gcccatgccg cgcgcgacga
     8881 cggccgcgtg gctggtcttg ccgccgcggc tggtcaggat gccctgcgcg gcgatcatgc
     8941 cgttgaggtc gtcggggttg gtctcgcgcc ggacgaggat gaccttctcg gtcgcggcgc
     9001 gctcctccgc gcggtcggcg tcgaagaccg cgacgcccac ggccgcgccc ggcgaggcgt
     9061 tcaggccggt ggcgagcacg gatccctcga accgctcctc gtccacctgc gggtgcagca
     9121 gcatgtcgat ctgctcgggc gtgacgcgac ggacggcttc cttctcgtcg atcagcttct
     9181 ccttgcgcat ctgcacggcg agcttcaccg cggccgcggc ggtgcgcttg ccgacgcgcg
     9241 tctggagcat ccacagcttt ttgcgctcga tggtgaactc gatgtcacac atgtcgcggt
     9301 agtgacgctc gagcttgttc atgatctgca acagctgctt gtaggccttg ccgttgatcc
     9361 gctcgagctc gctgatgggc aaggtgttgc ggatgcccgc cacgacgtcc tcgccttggg
     9421 cgttttcgag gtagtcgccg tagggctgcc ccgagtcgcc ggtcgccgga tcgcgtgtga
     9481 acgcgacccc ggttcccgag tcggcaccga gattgccgaa caccatcgcc tgcacgttga
     9541 cggcggtgcc gagctcgtcg gagatcttct cctggcggcg atagagcttc gcgcgatcgc
     9601 cgttccagga gtcgaacacg gccttgaccg ccagctccag ctgcttgcgt ggatcgttcg
     9661 ggaactgctg gccggtctct tccttgacga tgaccttgaa ctcgtccacc agctcgcgaa
     9721 ggtcctgctc gtcgaggtcg gtgtcgacgg caacaccctt gcgcttcttc gcttcgtcga
     9781 gcgcgtgctc gaagcgttcg ccgtcggcgc ccatgacgac cttgccgaac atctggatga
     9841 agcgccggta ggcgtcccac gcgaatcgcg ggttgtcgct gacgcgcgcg agcccgttga
     9901 cgctgcggtc gttcaagccg aggttgagga ccgtgtccat catgcccggc atcgagaact
     9961 tggctcccga gcgcaccgag accagcagcg ggtcgtcggg gtcgccgagc gtgcgtccca
    10021 tcgccttctc gagcgcgcgc aggtgcctct cgacctcgtc catcagattc ggcgggaacg
    10081 tgccggtgtg catgtactcg cggcacgcgt cggtggtgat ggtgaagccg ggggggaccg
    10141 gcaagcccat gccggtcatc tcggcgaggt tcgcgccctt gccgccgagc cggtccttca
    10201 tctctttgga tccttcggag aacgaggaga cgaacttgcc ggatcgcttc gccggcgcgc
    10261 tcgtcttctt ggccgccggg cgcttcaccg acgccggcgt agacgggcgc gaccgggctt
    10321 tcgccgcctt cgtggctcgc ttcgtcgtcg cggtcgtctt cggcttgctc cggcgcgcac
    10381 tgggcatacg ggtccctcct acggtctggt ggattcaaca tacccgccgg gccggatgac
    10441 acgcgagggt cgcagcagca caaaacggac gcgcgggcgc aatccgagac gggctacact
    10501 cgcgccgatg agcccgcgag tggaccgagt gtcgctccga acgccgcgtc tgacgctgat
    10561 gccgttcgca gaagcgcatg cggcggcggt ctgggccgcg atcgagccgt cgctcgagga
    10621 ccttcgtccg tggttggtgt ggactcgctg gacctcggag cgcgcgaccg ccgagttcgt
    10681 cgagagctgt cccggcgcgt ggcgcaacgg cagcggattc tcgttcgcgg tcttggaagg
    10741 cgaggacctg atcggatcgt gctctttggc gatccacaat ccgaatctga cgcagggcga
    10801 gatcggctac tggctgcgct cggaccgtgc cggcaacgga tacacgaccg aggccgcgcg
    10861 cgcggtgcgc gacttcgggt tcgacgcgac gggctgccac cggctcaacc tccgcgcggg
    10921 tgtcggcaac gtcgcgagcc agcgtgtcgc cgaaaagatc ggcttcaccc gcgaggggc
//
LOCUS       DATLFO010000162        10622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_141557, whole genome shotgun sequence.
ACCESSION   DATLFO010000162 DATLFO010000000
VERSION     DATLFO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141557"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="VM841_06220"
     CDS             <1..1716
                     /locus_tag="VM841_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HVL89815.1"
                     /translation="LEGVKHVFGIPGGTILPAYDPLLDSSIRHILCRHEQGAAHAASG
                     FAQATGEVGLCMATSGPGATNLVTGLADAYMDSVPMVAITGQVPRWAIGGDAFQECDA
                     TGITMPVTKHNWLIQDVQEIPQVIKEAFHIARTGRPGPVLVDIPKDLLQAEMAWWYPD
                     SVDLPGYKPVTKGNMKQIREAAKMLLAAKRPVIYAGGGIIKAGAAEELRRLAELSGAP
                     VVTTLMARGAIPDDHPLCLGMPGMHGNYTAVTAMQKADLLVALGARFDDRVTGKLDAF
                     APHAKIVHADIDPAEIGKNRPVDVPIVGDARSVIHDLVEALAAAIEDRGPASYEMWIA
                     TINEWREKFPYKYTQHADGPLKPEFVIEKIGELTRGDGIYVAGVGQHQMWASQYLKFK
                     RPNSWINSGGLGTMGFAVPAAMGAKAGRPGDQVWAIDGDGCFQMTSQELATMTTENIP
                     VKIAIINNGHLGMVRQWQELFYEERYSQVHLNGDVPDYVKLAEAYGAVGLRCETMDEV
                     EATINKAAEIDDRTVVIDFRVDQTAKCYPMVPAGASNDDIILGPEFEGQEPPKHMHDS
                     PEAIF"
     gene            1716..2237
                     /gene="ilvN"
                     /locus_tag="VM841_06225"
     CDS             1716..2237
                     /gene="ilvN"
                     /locus_tag="VM841_06225"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HVL89816.1"
                     /translation="MARHTLSVLVENKPGVLARVAGLFARRGFNIHSLAVGPTEIENI
                     SRMTIVVNVDTQPLEQVVKQLNKVIPVVKIVELEKDSSVERELLLIKVKADAHSRAQI
                     VEIADIFRSKIIDVAPNSLTIEATGSPDKLAALTDLLQTHGVREMVRTGLVALARGDK
                     AMADRQLRLEKGA"
     gene            2243..3247
                     /gene="ilvC"
                     /locus_tag="VM841_06230"
     CDS             2243..3247
                     /gene="ilvC"
                     /locus_tag="VM841_06230"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVL89817.1"
                     /translation="MAKMFYDGDADLAHLQGKTVSIIGYGSQGHAHALNLHESGVGVR
                     VGLAEGSKSWDKAAGDGLTVLPVKDAAAEGDVVMILVPDTSQRAVYDDAIAPGLADGK
                     ALAFAHGFNIHFGQIVPPAGVDVFMVAPKGPGHLVRRQYTEGGGVPGLLAVHQDATGK
                     ARDVGLAYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGTSELIKAGFDTLVEAG
                     YQPEVAYFECLHELKLIVDLLYEGGFEYMRYSISDTAEYGDYSRGSRVVTDATRAEMK
                     KILGEITSGEFAKEWIAENESGRPGFEKMRDEQAGHLIEKVGKELRAMMPWIAQKRTQ
                     "
     gene            complement(3306..4151)
                     /locus_tag="VM841_06235"
     CDS             complement(3306..4151)
                     /locus_tag="VM841_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89818.1"
                     /translation="MRKIIVLAAAAALTLSACGKTDQIKKPSEGGGAASNAVNVKAHE
                     YAFDVSGKPTTGSLSIEFANTGKELHHGIIGKLDEGKTLDDVNAFLKAGGQEPPEWFD
                     DSPTDVNLLSPGEKATITFEAKSAGTYVLLCFMPTPTGEPHVAKGMINTFEVAEGGDT
                     KAPKAAATIEMTEYTFSTPSVKAGDVSLEFKNAGKERHEFIVVQFAEGKKPADVDAWF
                     EGGLKGDAPATFYGGTHEIAPGESAFFTANLKAGTYTLVCGVETEDGKNHADDLGMMT
                     ELKIA"
     gene            complement(4237..5142)
                     /locus_tag="VM841_06240"
     CDS             complement(4237..5142)
                     /locus_tag="VM841_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HVL89819.1"
                     /translation="MAPPVISIAHDDRIQAAVQGALSRLDLDGAARGKLVAIKPNETW
                     ATKDDATGVTQPDTLRAVIRHLKKFDPRHIVVSGGAGAAETEDVMRHAGMLDVIKDED
                     VELFDHNRPPFVPVTLTYAPDKDVDGPQESVMVNPRVLEYEMLVSLAQIKMHETATVT
                     LALKNIAMSYPAADYYGHPRKSREHDNAFFDDMHSFIAAMAKRFPIGLAILAGHPAMV
                     GTGPLGGHAVETGIVVASTDPLAADVAAARMLGFRLQGVRHLWEAARLGVGDPNLDAA
                     TFPGLPLWDGAARFTEAIYGRALNL"
     gene            complement(5146..6171)
                     /locus_tag="VM841_06245"
     CDS             complement(5146..6171)
                     /locus_tag="VM841_06245"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HVL89820.1"
                     /translation="MTPTLTPQTRPATREALREIARLPDGPALSVIFNLDPREFGTAP
                     ARATAIRSLQDDVRRSAGDELQDEADAVMTYLQGGEFDQSKGRAVALFASSEAGYFET
                     MMLPEPLPTSVAALPYPGMAPVAHLMAAETWCVALVDRAAARILTGTARDLQEFEDAD
                     DDVHRRHSAGGGSQARWERSVEVEVDRHLEHVAEVITRAWRRGFDRLALGATAEMEPR
                     TLDALGGDERGRYSGRFDVDVQNTTVEEVADAAGVLFAMRDWERIREALGRAASRAGD
                     GLSASGPENVSSALEQMRVATLVVDPSLDRASFFVRQAALQDAVVLTAPAEPLREHQG
                     AVAVLRF"
     gene            6257..6739
                     /locus_tag="VM841_06250"
     CDS             6257..6739
                     /locus_tag="VM841_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVL89821.1"
                     /translation="MEGLDRAAVEALLLDAYPWARSLALMLTRNGFEADDLVQEAMSS
                     FLRKPPQVVTAATVHAWLRTAIVRDASRRRRRAASELKAFGRLFVQPPPVPATSQPSD
                     DMLQAVRRLPPKQRACIVLRYVQDQSEEQIAEILGIAPGTVKAHLAQARERLRDLLGD
                     "
     gene            6773..7555
                     /locus_tag="VM841_06255"
     CDS             6773..7555
                     /locus_tag="VM841_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HVL89822.1"
                     /translation="MRDDELRRRLRKLESELESGNIPLRPARPDARRGVRMRPAAAGL
                     GMAASIAATIALFLSVLPSPNDGISGVRPAAGSPANDALPDDPAPTIVTPDAVPIEPG
                     TPPSPTPTVPVAPTGGAPAADCFTPQGKDAGQGASITAETSPGQVRRGDTITLRAVFR
                     NTGGSTIENGEGNPRADFEVTDAGGSVVWVYSHGKAFTKELRLAEYAPGEEVAVETTW
                     NLRACGPDGADGPVIDSGTYRVRALWAGEHGGWSGFASFTVG"
     gene            7648..8793
                     /locus_tag="VM841_06260"
     CDS             7648..8793
                     /locus_tag="VM841_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HVL89823.1"
                     /translation="MRKPEILMTPGPTPVPAEVLLAQASPIVYHRGPGFGAVLRDCID
                     GLQWIMETRNDVLTFTTSGSGAMESAVVNCFSPGDKVLVVSVGNFGERFEKICRTYGL
                     DVQVIDYEWGKTAKAADVAAALTPDTKGVLIQHSETSTGVVNDIESIAEVVRDHPALF
                     IVDTVSGLGASPLRVDAWGIDVAAGGVQKALMMSPGIGFVSVSPKAWEAYENSTLPKF
                     YFDWGPVKKAHDMPEPESPYTPAVSLFLGLQAAIKLLREEGLENCFERHRTLARAVKA
                     AVQALGLELVGEDQDRAVCVTTIRAPEGIDGSSITKTVRAKFGIVFAPGQGQWKGKVF
                     RIGHLGYFERADVLQAIAALEMVLDELGYPLKQGAGVAAAQDVFAQA"
     gene            8790..10361
                     /gene="serA"
                     /locus_tag="VM841_06265"
     CDS             8790..10361
                     /gene="serA"
                     /locus_tag="VM841_06265"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HVL89824.1"
                     /translation="MRVLVAEKIAAAGIELLSAEYSVVDGTSMSRDELLAAIGDCDAL
                     IVRSATQADAELIGRAPNLKVIGRAGIGLDNVDVDVATKAGIVVVNAPTSNVISAAEH
                     TIALMLSQARNIPRADASMRAGKWERSRFEGVELHGKILAILGLGRIGTLVAQRALAF
                     GMKVIAFDPYVAKQRAAQMGVEVVETLEDALSRADFVTLHLPKTKETASLIGARELAL
                     MKPNARIVNASRGGMIDEAALAAAVREGTIGGAALDVYSSEPAPEDTPVRAEPNIVLT
                     PHLGASTEEAQEKAGVTIAEQVRLALRGELAQYAVNVDAGREISDTVRPFIGLAERLG
                     RLFVSVAGGSVTNVSLRYNGQIADHDTKVLTLSALRGMLGAVVTEPVTFVNAPLLAAE
                     RGIEYSETKSAAARDYVNVLEIRGEDGVGVAGTLVGTRNEERITRVYDFHLEMPPGEV
                     MCFLRYDDRPGVIGAIGTILGERSINIADMRVGRQERGGEALMALSVDTEIPAEVLDE
                     LGKGSGAKSAVVVVL"
     gene            10392..>10622
                     /locus_tag="VM841_06270"
     CDS             10392..>10622
                     /locus_tag="VM841_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL89825.1"
                     /translation="MDTPETLYANVGDDQVAYQTFGDGPDLVYSIGFLLSHVDHLWEL
                     PELARFLARLGSFCRVTLFDRRGMGASDALPAS"
BASE COUNT         1702 a         3518 c         3686 g         1716 t
ORIGIN      
        1 ctcgaggggg tcaagcacgt attcgggatc cccggcggca cgatcctgcc cgcctacgac
       61 cccctgctgg actccagcat ccgccacatc ctgtgccgtc acgagcaggg cgccgcgcac
      121 gccgcgagcg gcttcgcgca agcgaccggc gaggtcggcc tgtgcatggc caccagcggt
      181 ccgggcgcga cgaacctcgt gaccggcctg gccgacgcgt acatggactc cgtgccgatg
      241 gtcgcgatca ccggacaggt gccgcgctgg gcgatcggcg gggacgcgtt ccaggaatgc
      301 gacgcgaccg gcatcacgat gccggtcacg aagcacaact ggctcatcca ggacgtgcaa
      361 gagatcccgc aggtcatcaa ggaggcattc cacatcgcac ggaccggccg ccccggcccc
      421 gtgctcgtgg acatccccaa ggacctgctc caggccgaga tggcctggtg gtatccggat
      481 tcggtggacc tgcccggcta caagccggtc accaagggca acatgaagca gatccgcgag
      541 gccgccaaga tgctgctcgc cgcaaagcgt cccgtgatct atgcgggcgg tggcatcatc
      601 aaggccggcg cggccgagga gcttcgccgg ctagccgagc tgtccggcgc gccggtcgtc
      661 accacgctga tggcgcgcgg cgcgattccc gacgatcatc cgctgtgcct gggcatgccc
      721 ggcatgcacg gcaactacac cgcggtcacc gcgatgcaga aggccgacct gctcgtcgcg
      781 ctcggcgcgc gcttcgacga tcgcgtcacc ggaaagctcg acgcgttcgc gccgcacgcc
      841 aagatcgttc acgccgacat cgacccggcc gagatcggca agaaccggcc cgtggacgta
      901 ccgatcgtcg gtgacgcccg cagcgtgatc cacgacctcg tcgaggcgct cgctgccgcg
      961 atcgaagacc gcggccctgc gagctacgag atgtggatcg ccaccatcaa cgagtggcgc
     1021 gagaagttcc cctacaagta cacgcagcac gccgacgggc cgctcaagcc cgagttcgtg
     1081 atcgagaaga tcggcgagct cacccgcggc gacggcatct acgtcgccgg tgtcgggcag
     1141 catcagatgt gggcgtcgca gtatctgaag ttcaagcgcc ccaactcgtg gatcaactcc
     1201 ggcggcctgg gcacgatggg cttcgccgtt cccgccgcca tgggcgccaa ggcgggacgg
     1261 cccggcgatc aggtctgggc catcgacggc gacggatgtt tccagatgac ctcgcaagag
     1321 ctcgcgacga tgacgactga gaacatcccg gtcaagatcg cgatcatcaa taacggccat
     1381 ctgggcatgg tgcgccagtg gcaggagctc ttctacgagg agcgctacag ccaggtgcac
     1441 ctcaacggcg atgtgcccga ctacgtcaag ctcgccgagg cgtacggcgc ggtcggcctg
     1501 cggtgcgaga cgatggacga ggtcgaggcg accatcaaca aggcggccga gatcgacgac
     1561 cgcacggtcg tgatcgactt ccgcgtggac cagacggcca agtgctatcc gatggtgccg
     1621 gccggcgcgt ccaacgacga catcatcctc ggcccggagt tcgaaggcca ggagccgccc
     1681 aagcatatgc acgattctcc ggaggcgatc ttctaatggc caggcacacg ctctccgtcc
     1741 tcgtcgagaa caagccgggc gtgctcgcgc gcgtcgccgg gctcttcgcg cggcgcggct
     1801 tcaacatcca ctcgctcgcg gtcggtccga ctgagatcga gaacatctct cgcatgacca
     1861 tcgtggtcaa cgtggacacc cagccgctcg agcaggtggt caagcagctg aacaaggtga
     1921 tcccggtcgt caagatcgtc gagctcgaga aggactcgtc ggtcgagcgg gagctgctgc
     1981 tgatcaaggt caaggccgac gcgcactcgc gtgcgcagat cgtcgagatc gccgacatct
     2041 tccggtcgaa gattatcgac gtcgccccga attcgctcac gatcgaggcg accggctcgc
     2101 ccgacaagct cgcggcgctg acggacctgc tgcagacgca cggggtgcgc gagatggtgc
     2161 gcacgggcct ggtcgcgctc gcgcgaggcg acaaggcgat ggccgaccgg caactccggc
     2221 tcgagaaggg ggcatgagct tcatggcgaa gatgttctac gacggcgacg cggacctggc
     2281 gcacctgcag ggcaagacgg tttcgatcat cgggtacggc tcgcagggtc acgcgcacgc
     2341 gctcaacctc cacgagtcgg gcgtcggcgt ccgggtcggt ctcgccgaag gatcgaagtc
     2401 ctgggacaag gccgcgggcg acggcctgac ggtgctgccg gtcaaggacg cggccgccga
     2461 gggcgacgtg gtgatgatcc tagtgcccga cacctcgcag cgcgccgtgt acgacgacgc
     2521 gatcgcgccg ggcctggccg acggcaaggc actcgcgttc gcgcacggtt tcaacatcca
     2581 cttcggtcag atcgttccgc ccgcgggcgt ggacgtgttc atggtcgcgc ccaagggccc
     2641 cggtcatctg gttcggcgtc agtacaccga gggtggcggc gtgcccgggc tgctcgccgt
     2701 ccatcaggac gcgaccggca aagcccgcga cgtcggcttg gcctacgcca agggcatcgg
     2761 ggcgacccgt gcgggcgtga tcgagacgac cttcaaggaa gagaccgaaa ccgacctgtt
     2821 cggtgagcag gccgtgctgt gcggcggcac cagcgagctg atcaaggccg gtttcgatac
     2881 cctcgtcgag gcgggctacc agccggaggt cgcgtacttc gaatgcctgc atgagctgaa
     2941 gctgatcgtg gacctgctct acgaaggcgg cttcgaatac atgcgctact cgatcagcga
     3001 caccgccgag tacggcgact actcgcgcgg ctcgcgcgtc gtcaccgacg caacccgcgc
     3061 cgagatgaag aagatcctcg gggagatcac ctcgggcgag ttcgccaagg agtggatcgc
     3121 cgagaacgag tcgggccgtc ccggcttcga gaagatgcgt gacgagcagg ccggccacct
     3181 catcgaaaag gtcggcaagg agctgcgcgc gatgatgccc tggatcgccc agaagcgaac
     3241 ccagtagcgc ggcagaaacg aagcggccgg gagcatcgct cccggccgcg ctgctttccg
     3301 cgtcgctatg cgatcttgag ctcggtcatc atgccgaggt cgtcggcgtg gttcttgccg
     3361 tcctcggtct cgactccgca gaccagcgtg tacgtaccgg ccttgaggtt cgccgtgaag
     3421 aaggccgact cgccgggcgc gatctcgtgc gtgccgccgt agaaagtggc cggggcatcg
     3481 cccttcagcc cgccctcgaa ccaggcgtct acgtccgcgg gcttcttgcc ctcggcgaac
     3541 tggaccacga tgaactcgtg acgttccttg ccggcgttct tgaactcgag cgagacgtca
     3601 ccggccttga cgctgggcgt cgagaaggtg tactcggtca tctcgatagt ggcggcggcc
     3661 ttgggcgctt tggtgtcgcc gccctcggcg acctcgaagg tgttgatcat gcccttcgcg
     3721 acgtgcggct cgcccgtcgg ggtcggcatg aagcacagca gcacgtaggt gccggcgctc
     3781 ttggcttcga aggtgatcgt ggccttctca ccgggtgaga gcaggttcac gtcggtcggc
     3841 gagtcgtcga accattccgg cggctcctgg ccgcccgcct tcagaaacgc attgacgtca
     3901 tccagcgtct tgccttcgtc gagcttgccg atgatgccgt ggtgcagctc tttgccggtg
     3961 ttggcgaact cgatcgacaa tgaaccggtc gtcggcttgc cgctcacgtc gaacgcgtat
     4021 tcgtgtgcct tgacgttcac ggcgttcgag gcggcaccgc cgccctccga cggcttcttg
     4081 atctggtcgg tcttgccgca cgcgctgagc gtgagcgccg cggcggcggc aaggacgatg
     4141 atctttcgca tgcactatct ccctgtgtcg gctccggccg aggcgaccgg gcgcgatgac
     4201 cgtatcaggg ccgttcgccg ggcgccatca gccttgctaa aggttgaggg ctcggccgta
     4261 aatcgcttcg gtgaagcgag cggcaccatc ccacagcggc agaccgggaa aggtagccgc
     4321 atcgaggttc gggtcgccga cgccgagccg tgcggcctcc cacagatgac gcacgccctg
     4381 cagccggaat cccagcatgc gcgcggcggc gacgtccgcg gcgagcggat ccgtcgatgc
     4441 aaccacgatg cccgtctcga ccgcgtgccc gccgagcggg ccggtgccga ccatcgcggg
     4501 atggcccgcc aggatggcca agccgatggg gaagcgcttg gccatggcgg cgatgaacga
     4561 gtgcatgtcg tcgaagaacg cgttgtcgtg ctcacggctc ttgcggggat gcccgtagta
     4621 gtcggcggcg ggataggaca ttgcgatgtt cttgagcgcc agcgtcacgg tcgcggtctc
     4681 gtgcatcttg atctgcgcga gcgacacgag catctcgtat tccaggaccc gcgggttcac
     4741 catgacggat tcctgcgggc cgtccacgtc cttgtcgggc gcgtaggtca gggtcaccgg
     4801 gacaaagggc gggcggttgt ggtcgaacag ctcaacgtct tcgtccttga tcacgtcgag
     4861 catgccggcg tggcgcatca cgtcctcggt ctcggccgcg cccgcgccgc cgctgacgac
     4921 gatgtgccgg ggatcgaatt tcttcagatg gcggatcacg gcgcgcagcg tgtccggctg
     4981 ggtcacaccg gtcgcgtcgt ccttggtcgc ccaggtctcg ttgggcttga tcgcgaccag
     5041 ctttcctctg gccgcgccgt ccaggtccag gcgcgacaag gcgccctgga cggccgcttg
     5101 gatacggtcg tcgtgcgcga tcgagatcac gggcggtgcc acgggtcaga accgtagcac
     5161 tgccaccgcc ccctggtgct cacgaagggg ctctgcgggc gcggtcagca cgaccgcatc
     5221 ctgaagcgcg gcctgccgga cgaagaacga agcgcgatcc agcgacgggt cgaccacgag
     5281 cgttgcgacc cgcatctgct cgagcgccga ggacacgttc tccgggcccg acgcgctcaa
     5341 gccgtcgccg gcgcgcgagg cggcacggcc gagggcctcg cggattcgct cccagtcgcg
     5401 catcgcgaac agcacgcccg ccgcgtcggc gacctcctcg acggtggtgt tctgcacgtc
     5461 cacgtcgaac cggccgctgt agcggccgcg ctcgtcgccg ccgagcgcgt cgagggtgcg
     5521 cggctccatc tcggcggtcg caccgagcgc gagccggtcg aagccgcggc gccaggcgcg
     5581 agtgatcacc tccgcgacgt gctcgaggtg gcgatcgacc tcgacctcga cggagcgctc
     5641 ccagcgcgcc tgcgacccac cgccggcgct gtgccggcgg tgcacgtcgt cgtcggcgtc
     5701 ttcgaactcc tgaaggtcgc gcgccgtgcc cgtcaggatg cgggccgcgg cgcggtcaac
     5761 cagcgcgacg caccacgtct cggcggccat caggtgcgcg accggagcca tcccggggta
     5821 gggcagcgcc gcaaccgagg tcggcagcgg ctcgggcagc atcatcgtct cgaagtatcc
     5881 ggcctcgctc gaggcgaaca gcgccacggc ccggcccttc gactggtcga actcgccgcc
     5941 ttgcagatac gtcatcaccg catcggcctc gtcctgaagc tcgtcgccgg cggagcgccg
     6001 cacgtcgtct tggagcgagc ggatcgcggt ggctcgcgcg ggagcggtgc cgaattcgcg
     6061 gggatcgagg ttgaagatca ccgacagagc agggccgtcc ggcaggcgag cgatctcgcg
     6121 gagcgcctcg cgcgtggccg ggcgtgtctg gggagtcagc gtgggagtca tgttccggtc
     6181 ttacccgttc gccgggactg ctacccccgg ccggcatgcc ggggtcggca tgatcgggcc
     6241 ggtatcgttt cgctccatgg aggggctgga tcgcgcggcc gtcgaagctt tgctgctgga
     6301 tgcctatccc tgggcccgct cgctcgcttt gatgctgact cgcaacggtt tcgaggccga
     6361 cgacctcgtt caggaggcca tgagctcgtt tctgcggaag ccgccgcagg ttgtgaccgc
     6421 ggcgaccgtg cacgcgtggc tgcgcaccgc gatcgtgcgc gacgcaagcc gccgccgccg
     6481 ccgcgccgca agcgagctga aggcgttcgg ccgcctgttc gtccagccgc ctccggtgcc
     6541 cgctacgagc cagccgagcg acgacatgct gcaggccgtt cggcggcttc cgccgaagca
     6601 gcgtgcgtgc atcgtgctcc gctacgtgca ggaccaaagc gaggagcaga tcgccgagat
     6661 cctcgggatc gccccgggca cggtgaaggc gcatctggcc caggcccgtg agcggcttcg
     6721 ggatctgctc ggcgactaac ccggccgggc ccgtccccgc tctatagctg cgatgagaga
     6781 cgacgagctt cgccggcggc tccgcaagct ggagtccgag ctggagtccg gcaatattcc
     6841 gctgcggccc gcgcgtccgg atgcgcgccg cggcgtgcgg atgcgtcccg cggccgcggg
     6901 cctgggcatg gcggcctcga tcgcggccac gatcgcgctg ttcctgtcgg tcctgccgag
     6961 cccgaacgac ggcatctcgg gcgtccgtcc cgccgccgga tcgccggcga acgacgcgct
     7021 gccggacgat cctgccccga ccattgtgac gcccgatgcg gttccgatcg agccggggac
     7081 cccgccttct ccgacaccga cggtgcctgt cgcgccgacc ggtggggctc cggcggcgga
     7141 ctgcttcacc ccccaaggca aggacgcggg ccagggcgcg tcgatcacgg ccgaaacgtc
     7201 cccgggacag gtccgtcgcg gcgacacgat cacgttgcgc gcggtctttc gcaacaccgg
     7261 cgggtccacg atcgaaaacg gtgagggcaa tccgcgcgcc gacttcgagg tcacggacgc
     7321 cggcggctcc gtcgtgtggg tctacagcca cggcaaggcc ttcacgaagg agcttcgcct
     7381 ggccgagtac gcgcccggcg aggaggtcgc ggtcgagacc acctggaacc ttcgggcctg
     7441 cggtcccgac ggagcagacg gcccggtgat cgattccggc acgtaccggg tccgggcctt
     7501 gtgggccggc gagcacggcg gctggtcggg tttcgcctcc ttcaccgttg gctgagcagc
     7561 gattcgtgcg aaatccgatt cggcatcggc cctgccgcgc cctagaatgg gccccgagcc
     7621 cgaccgatcg aaagaggtgt ttcgcgcgtg cgcaagcccg agatcttgat gacccccggc
     7681 ccgacgccgg ttcccgccga agtcctgctc gcgcaggcct cgcccatcgt ctaccaccgc
     7741 ggtcccggct tcggcgcggt cctgcgcgac tgcatcgacg gcctgcagtg gatcatggag
     7801 acgcgcaacg acgtgctgac gttcacgacc tcggggtcgg gcgcgatgga gtctgccgtc
     7861 gtgaactgct tcagcccggg cgacaaggtg ttggtggtct cggtcggcaa cttcggtgag
     7921 cgtttcgaaa agatctgtcg cacgtacggg ctcgacgttc aggtaatcga ctacgagtgg
     7981 ggcaagaccg cgaaggccgc cgatgtcgcc gccgcgctga cgcccgacac caagggcgtc
     8041 ctgatccagc acagcgaaac ttcgaccggc gtggtcaacg acatcgagtc gatcgccgag
     8101 gtcgtgcgcg accatccggc gctgttcatc gtggatacgg tcagcggttt gggcgcgtcg
     8161 ccgcttcgcg tggatgcctg ggggatcgac gtcgccgccg gcggcgtgca gaaggcgctc
     8221 atgatgtcgc cgggcatcgg cttcgtgtcg gtctcgccca aggcgtggga ggcctacgaa
     8281 aactcgacgc tgccgaagtt ctacttcgat tggggtccgg tcaagaaggc ccacgacatg
     8341 cccgaacccg agtcgccgta tacgccggcc gtgtcgctgt tcctgggtct gcaggcggcg
     8401 atcaagctgc tgcgtgaaga gggcttggag aactgcttcg agcgtcaccg cacgttggcg
     8461 cgggccgtga aggccgccgt gcaggcgctg ggcctggaac tggtcggtga ggatcaggac
     8521 cgcgcggtgt gcgtgacgac catccgcgcg ccggagggca tcgacgggtc gtcgatcacg
     8581 aagacggtgc gtgccaagtt cgggatcgta ttcgcgccgg gtcagggaca gtggaagggc
     8641 aaggtcttcc gcatcggcca tctcggctac ttcgagcgcg ctgacgtgct gcaggccatc
     8701 gccgcgctcg agatggtgct cgacgagctc ggctacccgc tcaaacaggg tgcgggggtt
     8761 gcggccgcac aagacgtgtt cgctcaggca tgagggtcct cgtcgccgaa aagatcgcgg
     8821 ccgccgggat cgagctgctg tcggcggagt attcggtcgt ggacggcacg tcgatgtcgc
     8881 gagacgagct gctcgccgcg atcggcgact gcgatgcgct catcgttcga tcggccacgc
     8941 aggcggatgc cgagctgatc ggccgcgcgc cgaacctgaa ggtcatcggc cgtgccggga
     9001 tcggcctgga caacgtcgat gtggatgtgg cgaccaaggc cggtatcgtt gtggtgaacg
     9061 cgccgacgtc gaacgtgatc tcggctgccg agcacacaat cgcgctgatg ctgtcccagg
     9121 cgcgcaacat tccgcgcgcc gacgcctcga tgcgcgccgg aaagtgggag cgatcgcgtt
     9181 tcgagggcgt cgagctgcac ggcaagatct tggcgatcct gggcttgggg cggatcggca
     9241 cgctcgtcgc gcagcgcgcg ctggcgttcg ggatgaaggt gatcgccttc gatccgtacg
     9301 tcgccaagca gcgtgccgcg cagatgggcg tcgaggtcgt cgagacgctc gaggacgcgc
     9361 tgtcgcgcgc ggatttcgtg acgctgcatc ttccgaagac gaaggagacc gcgtcgctga
     9421 tcggcgcgcg cgagctggcg ctgatgaagc ccaacgcgcg catcgtcaac gccagccgcg
     9481 gcggcatgat cgacgaagcg gcgctggcgg ccgcggtgcg tgaaggcacg atcggcggag
     9541 cggcgctgga cgtgtattcg tccgagccgg cgcccgagga cacgccggtg cgcgccgagc
     9601 cgaacatcgt gctgaccccg catctcggtg cctcgaccga agaggcgcaa gagaaggccg
     9661 gtgtgaccat cgccgagcag gtgcggctgg cactacgcgg cgagctcgcg cagtacgcgg
     9721 tcaacgtgga cgcgggccgc gagatcagcg acaccgtgcg gccgttcatc ggactggccg
     9781 aaaggctcgg ccggctgttc gtttcggtgg cgggcggaag cgtgaccaac gtgtcgcttc
     9841 gctacaacgg tcagatcgcc gatcacgaca ccaaggtact caccttgtcg gcgctgcgcg
     9901 gcatgctcgg cgcggtcgtg accgagccgg ttaccttcgt caacgcgccg ctgcttgccg
     9961 ccgagcgcgg catcgagtac agcgagacca agtccgccgc tgcgcgcgac tacgtcaacg
    10021 tgctcgagat ccgcggtgag gacggtgtcg gtgtcgcagg gacgctggtc ggcacccgca
    10081 acgaagaacg gatcactcgc gtgtatgact tccatctgga gatgccgccg ggcgaggtca
    10141 tgtgctttct ccgctacgac gaccgtccgg gcgtgatcgg cgcgatcggc accattcttg
    10201 gtgagcggag catcaacatc gccgacatgc gcgtgggccg ccaagagcgt gggggagagg
    10261 ccttgatggc cttgtcggtg gacaccgaga tcccggccga ggtgctcgac gagctgggca
    10321 agggctcggg cgcgaaaagc gccgtcgtcg tggtcctcta aatcgcgagc ggtttgttac
    10381 gctggtgcgc tgtggacacg ccggaaacgc tgtacgcgaa cgtcggtgat gaccaggtcg
    10441 cgtaccagac gttcggtgac ggtcccgatc tcgtctattc gatcgggttc ctgctcagtc
    10501 acgtcgatca cctatgggag cttcccgaac tcgcgcgatt tctggcacgc ttgggctctt
    10561 tctgccgagt gactctgttc gatcggcgcg gcatgggagc ctccgatgcg cttccggcct
    10621 cc
//
LOCUS       DATLFO010000163         5861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_73203, whole genome shotgun sequence.
ACCESSION   DATLFO010000163 DATLFO010000000
VERSION     DATLFO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73203"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VM841_06275"
     CDS             <1..1015
                     /locus_tag="VM841_06275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVL89826.1"
                     /translation="PLAVPLRLGAALLGEERGLASRRATRPDEAARGRSGAGTPVPGK
                     GRTRTGAAGKGATSSGATGKGAAGRVPSKRQAKPPKPRARPSDGPPPPPLGEPGEAIR
                     LQRVLSAAGVASRRAAEDLITSGRVSVDGKVVRTLGTRVDPETARIEVDGERIGVRRN
                     RQYLIMNKPVGVITSASDPFGRKTVLDLVRVRGRVFPVGRLDSDTSGLLLLTDDGELA
                     HRLAHPRYGIEKVYVAKVAGRVETATLKRLEKGIRLDDGPARAVRARLRGKASKTSQV
                     EIVMAEGRKREVRRMLEAVGHPVDELVRVAYGPIRLSGLVTGDVRELTIAEIGELYRL
                     VGL"
     gene            1082..1447
                     /gene="aroH"
                     /locus_tag="VM841_06280"
     CDS             1082..1447
                     /gene="aroH"
                     /locus_tag="VM841_06280"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HVL89827.1"
                     /translation="MERLRALRGATTLEADTRDQVIDRTGTLLKEMMERNAVDHEDIV
                     SIIFTATDDIHSEFPAAAARSIGLSTVPLLCARELSVEEGVTLCIRVLMHCYSNTPRD
                     GLRHVYLERAKPLRTDLPQ"
     gene            1449..2540
                     /locus_tag="VM841_06285"
     CDS             1449..2540
                     /locus_tag="VM841_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HVL89828.1"
                     /translation="MRVALLGSGLIGGSIGLALRRLDSVAEVVVYDRSEEAARRAVER
                     GAADRHEATPAEAVREADVIFVATPVGAIANTIKAAIPGLKSGAILTDVGSTKSRVVD
                     EVTSILGANVTFLGGHPMAGSEEDGIEGAKEGLFDGAWWILTPTPRVEPAAYRTLHGL
                     LSGMGCHVIALEPALHDELMAIISHLPHLTAATLMNMAADHGKDKASLLSLAAGGFRD
                     VTRVAASNPGIWIDICRENADAIVRALGEFIDRLSELRDFVARGATDDLQRRLESARL
                     ARRALAGKRTDGELFEVFIPIPDRPGVLAEVTTLIGNAGVNIEDIQISHAEEGGRGVL
                     RILILGEDESTKVHSALSSKGYQPRSTSI"
     gene            3038..4357
                     /gene="aroA"
                     /locus_tag="VM841_06290"
     CDS             3038..4357
                     /gene="aroA"
                     /locus_tag="VM841_06290"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HVL89829.1"
                     /translation="MRPAERVEGDLAVPGDKSISHRALMIAAAGHGDCTLIGLSSAHD
                     VASTLGAIRHLADVLGPDLPSLEVEGLAHSGSHWEIMVRGRGIEAWRNPGRTLDCGNS
                     GTTMRSLLGLLAACNFQATLDGDDSLRRRPMERVAAPLRRMGASITTGAAGRPPVVIR
                     GSVLTGIDHELPVASAQVKTALLFAGLHAEGETTVRGGGASRDHTERMLRHLGVDITG
                     SPNHLVVKSTQIQNAESLNIPGDLSSGAFLLVAAAIRPGSDLRVRNVGLNPTRTGILE
                     VLRSYGADVRIEDEAEVCGEPRGTVRIISGDRRPLHIDAHHVPALIDELPLVAILGAF
                     AEGPTVIEGAAELRVKESDRIAATAAGLEALGVRVETAPDGLTVTGDGRAAGGVVDSA
                     GDHRIAMAFSAAAIGADEPVTIRHWEAVDISYPSFGADLDSIAVRPA"
     gene            4354..5019
                     /gene="cmk"
                     /locus_tag="VM841_06295"
     CDS             4354..5019
                     /gene="cmk"
                     /locus_tag="VM841_06295"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVL89830.1"
                     /translation="MTRPIVIAIDGPAGAGKSSVSSGVARRLGIARLDTGSMYRAVTW
                     AALRDGTNLDDETALASLARRLDLSFDVSGILIDGRPRETEIRDPAVTASVSKVSRHP
                     AVRAALVSRQQKLAEQGPAVVEGRDIGTVVVPWAPVKIFLTASARERARRRAKDLEAA
                     GHEVDLDRLQAEIEARDAADDKTTPLVPAGDAVAIDSTAKTIDEVVDEIAALAAAAGL
                     GVR"
     gene            5016..>5861
                     /gene="der"
                     /locus_tag="VM841_06300"
     CDS             5016..>5861
                     /gene="der"
                     /locus_tag="VM841_06300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVL89831.1"
                     /translation="MSESRRPVVAVVGRTNVGKSTLVNRLLGRREAIEHDMPGVTRDR
                     QSYPVEWGRRSFLLVDTGGWEPRARGLAAKVVEQAERAAAQADLIVFVVDASTGAIGD
                     DLVVARSLRRAKVPVIVVANKVDSAAMEDELHALQRLGLGDALPISALHGRGSGDVLD
                     AIVATLPDEPAGPPEPEDRPLRVAVVGRPNAGKSSLFNRLTGQDRSIVDPTPGTTRDT
                     VDETVTIGERVYEFIDTAGMRRRARQAEGPEYYGLVRSLRAIDRADVAIIVIDAEAGV
                     TDQDQR"
BASE COUNT          972 a         1991 c         1974 g          924 t
ORIGIN      
        1 gcccttggcc gttccgcttc ggctcggcgc ggcattgctt ggggaggagc gtggcttggc
       61 atcgcggcgc gcgacccggc cggacgaggc cgcccgcggc aggagcggcg cgggcacgcc
      121 ggtaccgggc aagggccgca ccagaaccgg cgctgccggc aagggcgcca cgagcagtgg
      181 ggcgacgggc aagggcgccg ccggccgggt tccttcgaaa cggcaggcca agccgcctaa
      241 gccccgggcg cgcccgtccg atggaccgcc tccgccgccc ctcggcgaac cgggggaggc
      301 aatccgcctc cagcgggtcc tgtcggctgc gggggtcgcg tcccgccggg ccgcggagga
      361 tctgatcacg tcgggacggg tgagcgtcga cggcaaggtg gtccggacgc tcggcacccg
      421 ggtagatccc gaaacggccc ggatcgaggt ggacggtgag cggatcggcg tccgccgcaa
      481 ccgccaatac ctcatcatga acaagccggt cggcgtgatc acgagcgcga gcgacccctt
      541 cggccgcaag accgtgctcg acctggtccg ggttcgtggc cgcgtcttcc cggtcgggcg
      601 gctcgactcc gacacatcgg ggctgctgct cctgaccgac gacggcgagc tcgcacaccg
      661 gctcgcgcac ccacgctacg ggatcgagaa ggtctacgtc gccaaggtcg ccggacgggt
      721 cgagaccgcg acgctgaagc ggctcgagaa aggcatccgt ctcgacgacg ggccggcccg
      781 cgccgttcgc gcccggctcc gcggcaaggc ctccaagacc agccaggtcg agatcgtcat
      841 ggccgaggga cgcaagcgag aggttcgaag gatgctcgaa gccgttggcc atccggtcga
      901 cgagctcgtc cgcgtcgcct acggtccgat ccgcctatcc ggcctggtca cgggggatgt
      961 gcgagagctc accatcgccg agatcgggga actttatcga cttgtcggct tatagccttg
     1021 gcgatggatc cgtatctctt cttgcgtccg tagaggttca gtgccgacta tatttcccgc
     1081 catggaacgg cttcgcgcac tccgaggagc gacgaccctg gaagcggata cccgcgacca
     1141 agtcatcgat cgaaccggca cgttgctcaa ggagatgatg gagcgaaacg ccgtcgatca
     1201 cgaggacatc gtcagcatca tcttcacggc gaccgacgat atccacagcg agtttccggc
     1261 tgccgcggcc cgaagcatcg gcctctcgac cgttccgctg ctctgcgccc gcgagctcag
     1321 cgtggaggag ggcgtgacgc tgtgcatccg ggtgcttatg cactgctact ccaacactcc
     1381 gcgcgacggc cttcgccacg tgtacctcga gcgggccaag ccgctccgga ccgacctccc
     1441 tcagtaacgt gcgcgtcgcg ttgctgggat cgggcctcat cggcggatca atcggcctgg
     1501 cccttcgacg gctggactcg gtagcggagg tcgtggtcta cgatcgttcc gaggaagccg
     1561 ctcgcagggc ggtggaacga ggtgccgccg atcgccacga agcgacgccc gccgaggctg
     1621 tgcgcgaagc cgacgtgatc ttcgtcgcca cgccggtcgg ggctatcgca aatactatca
     1681 aagcggctat tccgggctta aaatcgggcg cgattctgac agatgttgga agcacaaagt
     1741 ctagagtcgt ggatgaggtc acatctatcc tcggtgcgaa cgttactttc ctcggcggcc
     1801 acccgatggc cggctccgag gaggacggca tcgaaggagc gaaagaaggg ctcttcgacg
     1861 gggcctggtg gatcctcacg cccaccccgc gcgtcgagcc tgcggcctac cggacgctcc
     1921 acgggctgct gtcgggcatg ggctgccacg tcatcgcgct cgagcctgca ctgcacgacg
     1981 aactcatggc catcatctct cacctgccgc acctcacagc ggccacgctg atgaacatgg
     2041 ccgccgatca cgggaaggac aaagcaagcc ttctgtccct cgcagctggt ggttttcggg
     2101 acgtaacgcg tgtcgccgcc tccaatccgg gcatctggat cgacatttgc agggaaaacg
     2161 ccgatgccat cgtccgcgcc ttgggcgagt tcatcgatcg gctgagcgaa ttgcgtgatt
     2221 tcgtggctcg cggagccaca gacgacctgc aacggcggct ggagtcggcc cggctggcac
     2281 gacgagccct cgccggcaaa cggacggatg gcgagctgtt cgaggtcttc atccccatcc
     2341 cggatcgccc gggcgtgctc gccgaggtga cgacgctaat cggaaacgca ggcgtcaaca
     2401 tcgaggacat ccagatcagc catgccgaag aggggggccg gggcgtgctt cgcatcctga
     2461 tcctgggtga ggacgaatcg acgaaggtcc acagcgcctt atcgtcgaag ggatatcaac
     2521 ctagatcgac ttccatctaa gtcgatacat cccttcatct agtatgcaat ggatcagtaa
     2581 agggctggtc acaggccata tatggattgc tttatcgata taacacctca cctagatacc
     2641 attgaagcga taaagcactt tttatcttca tcgattaagc gatgaagatc tctggaggtt
     2701 catagatcca gcgtctgtcc ccccgcataa acacgtgact tggtggatag attcctcaag
     2761 ggttatatct cggggttgat agattgattc attgctttac cgccgggtct accagtcacc
     2821 aagcctccac gaccgcacgc ccgaacgggc cgggccctcc cccgggcgga taggccgccg
     2881 ggctgcagga ctcgtttatg atccgtttgc tcgagcgggc ctgtgctcgt cgaatcgccg
     2941 cgcgccgggg cgccgacgat ccctcggacc cggcggcgcc cgtgcgggtc cgtacgagca
     3001 attcagggag caggagggca ggttggcgga gcacacggtg agaccggcgg agcgtgtcga
     3061 gggcgacctc gccgtgcccg gggacaagtc catcagccac cgcgcgctca tgatcgcggc
     3121 cgccggccat ggtgactgca ccctgatcgg gctcagctct gcccatgacg tggcgagcac
     3181 cctgggggca attcgccacc tcgcggacgt cctcggcccc gatctacctt cacttgaggt
     3241 tgagggtttg gcccattcag ggtcgcattg ggaaattatg gttcgcggac gcggcattga
     3301 agcctggaga aaccccggcc gaacgctcga ttgcggcaat tcgggcacga cgatgagatc
     3361 gctcctcggt ctgctcgccg catgcaactt tcaagcaacc ctcgacggag acgattcgct
     3421 gcgccggcga ccgatggagc gcgtggccgc gccgcttcgc cgaatgggcg cgagcatcac
     3481 gaccggagcc gcggggcggc cgccggtggt gatccggggg agcgtgctga ccggcatcga
     3541 ccacgagctc ccggttgcca gcgcacaggt caagacggcg ttgctcttcg ccggcctgca
     3601 cgcggagggt gagaccaccg tgcggggcgg gggagccagc cgagaccata ccgagcgcat
     3661 gcttcgccat ctcggtgttg atatcaccgg atctcctaat cacctagtcg ttaaatcgac
     3721 acagatccaa aatgctgaat cgctgaatat ccccggggat ttgtcgtcag gggcattcct
     3781 gctggtcgcg gccgccatcc ggccgggctc ggacctgcgg gtccgcaacg tcggactcaa
     3841 tccgacccgc accggcatac tcgaggtgct ccgctcgtat ggagcggatg tccgcatcga
     3901 agacgaggca gaggtctgcg gcgaaccgcg cgggaccgtc cgcatcatct ccggcgatcg
     3961 ccggcccctc catatagacg cgcaccacgt cccggccttg atcgacgagc ttcccctcgt
     4021 cgcgatcctc ggcgcgttcg ccgaggggcc gacggtcatc gagggggcgg ccgagcttcg
     4081 cgtgaaggag agcgaccgga tcgctgccac ggccgccggg ctcgaagcgc tcggcgtgcg
     4141 ggtcgagacg gctcccgacg gtctgaccgt caccggcgac ggccgggcgg cgggcggcgt
     4201 ggtcgattcg gccggggacc atcgcatcgc gatggcgttc tcggctgccg ccatcggcgc
     4261 cgacgagccc gtcacgatcc ggcattggga agccgtcgac atctcgtatc cgtcgttcgg
     4321 cgcggacctc gactcgatcg ccgtccggcc agcatgaccc gtccgatcgt gatcgccatc
     4381 gacgggccgg ccggggccgg caagtcgagc gtgtccagcg gggtggcccg gcggctcggc
     4441 atcgcgcggc tcgacaccgg ctcgatgtac cgggccgtca cctgggcggc tctccgggac
     4501 ggcaccaacc tcgacgacga gacggcgctc gcctcactcg cccggcggct cgatctttcc
     4561 ttcgacgtct cgggcatcct gatcgacggc cggccgcgag agaccgagat ccgggatccg
     4621 gccgtgacgg cctcggtttc gaaggtctcc cgtcacccgg ccgtgcgcgc tgcgctggtc
     4681 tcgcggcagc agaagctcgc cgagcagggc cccgccgtgg tcgagggccg ggacatcggg
     4741 acggtcgtcg tgccgtgggc tccggtcaag atcttcctca ccgcctccgc gcgcgaacgg
     4801 gcccgccgcc gtgccaagga tctggaagcg gccggccacg aggtggacct ggaccgcctg
     4861 caagccgaga tcgaggcgcg cgacgcggcc gacgacaaga ccacgccgct ggttccggcc
     4921 ggcgacgcgg tggcgatcga ctccaccgcc aagacgatcg acgaggtcgt cgacgagatc
     4981 gccgcgctcg ccgccgcggc agggctgggg gtccggtgag cgaaagccgc cgccccgtgg
     5041 tcgccgtcgt tggccgcacc aacgtcggca agtccacgct cgtcaaccgg ctgctgggcc
     5101 ggcgcgaggc gatcgagcac gatatgccgg gcgtcacccg ggaccgccag tcctatccgg
     5161 tggagtgggg cagacgctcg ttcctgctcg tggacaccgg cggctgggag ccgcgcgcga
     5221 ggggtcttgc cgcgaaagtg gtcgagcagg ccgagcgggc cgctgcccaa gccgacctga
     5281 tcgtgttcgt cgtggacgcc tccaccggcg cgatcggcga cgacctcgtc gtggcccgct
     5341 cgctccgccg cgcgaaggtg cccgtgatcg tcgtggccaa caaggtggac tcggccgcga
     5401 tggaggatga gctgcacgcg ctgcagcgcc tcggcctcgg cgacgcgttg cccatcagcg
     5461 cgctgcacgg ccgcggatcc ggcgacgtgc tcgacgccat cgtcgccacc cttcccgacg
     5521 agcccgccgg gccgcccgag cccgaggacc gtcccttgcg cgtcgcagtc gtcggccgtc
     5581 cgaacgcggg caagtcgagc ctgttcaacc ggctcaccgg ccaggaccgc tccatcgtgg
     5641 atccgacccc cggcacgacg cgagacaccg tcgacgagac cgtgaccatc ggcgagcgcg
     5701 tctacgagtt catcgacacc gccggcatgc ggcgccgcgc ccggcaggcc gaaggccccg
     5761 agtactacgg gctcgtgcgc tcgctccgcg cgatcgaccg cgccgacgtc gcgatcatcg
     5821 tcatcgacgc cgaagcaggc gtgaccgacc aggaccagcg g
//
LOCUS       DATLFO010000164        15757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_46722, whole genome shotgun sequence.
ACCESSION   DATLFO010000164 DATLFO010000000
VERSION     DATLFO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46722"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VM841_06305"
     CDS             <1..534
                     /locus_tag="VM841_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL89832.1"
                     /translation="GAGSGSNFPFYPPEVTQVAAVEPEPYLRGRAEEAAKKAPVPVTV
                     HAATAASLPFPDASFDAGVFCLVLCSVPSQPEALAEMYRVLKPGGELRFYEHVAAAPG
                     KMLRRVQQASDTVWPRLFGGCHTRRDTVSAIRDAGFEIDRVKDLSLTIAGVPSPITPH
                     AAGVARRPQASKPAAAE"
     gene            585..1049
                     /locus_tag="VM841_06310"
     CDS             585..1049
                     /locus_tag="VM841_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL89833.1"
                     /translation="MSTLMSSINLDVPVQTAYNQWTQFEEFPKFMDGIESVTQIDDVT
                     LRWVAKIDGVQREWTSEIVEQKPDDIIAWINHEGPNTAGSVKFEPSASGGCKVEVTMK
                     YDPDTFTEKAAETLGIIQRRLDGDLRRFKDFIEERGRETGAWRGEVHTAQVE"
     gene            complement(1109..1432)
                     /locus_tag="VM841_06315"
     CDS             complement(1109..1432)
                     /locus_tag="VM841_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVL89834.1"
                     /translation="MSPDTNKRAHVEVLLEAGALIVEPAAYRVWFGGNEVALTSTQVE
                     LVALLIANRDRVVAREEIARSLGLRHARSVDVLLTTVRHSLGRNFVRNVRSRGWILDE
                     ASLAA"
     gene            complement(1429..2034)
                     /locus_tag="VM841_06320"
     CDS             complement(1429..2034)
                     /locus_tag="VM841_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVL89835.1"
                     /translation="MEPARTIDGESAANLGLYQTRLVADHEGAEQWTVRVALRDRRGA
                     LAAIASSFAGAGIAVCSAHIATGPGGVVIDVFRVDASEWTDWDRVRSRIDAGLVAGYG
                     RTPSEAVDATLAIAPDGTGARSIIEVRAEDRVGLLAKVAAAISRAGVEIHQATITTIG
                     TEAIDVFEVTGRTGAALTAADKRALRAAFAGKRVRRSVRRR"
     gene            complement(2059..3510)
                     /locus_tag="VM841_06325"
     CDS             complement(2059..3510)
                     /locus_tag="VM841_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVL89836.1"
                     /translation="MRKRRILTVGALTAVFLIVTATAAFAQTADGPSEEKILLDTVWT
                     LMAAFMVFLMQGGFAMVEAGFTRKKNAGNIVMKNLMDFGMSSIAFWAVGFALMFGTGN
                     ALIGTSGWFSSDPTAFSSLDWATPPLMAKWLFQVVFAGTAATIVSGAMAERTKFKAYL
                     LYSGLISGLIYPIAGHWIWGGGWLASKEFLDFAGSTVVHSVGGWAALVGAIMVGPRLG
                     KYAKDGRPRPMPGHSMPLGMLGVFLLWFGWFGFNPGSTMAATLSIADIAVTTNLAAAA
                     GAIMAMTASWMLGRKPDVGMSANGALAGLVAITAGCAFVENWAALVIGAIAGVVVVGA
                     IMFFDRVKVDDPVGAVSVHGVCGVLGTLMVGVFASPRLIDAAGVGSPGLVYGGGMAQL
                     GVQALGAAAVMGWVLVASAVVFGLVKATVGLRVSAEEEMEGLDIGEHGMWGYPEEHLG
                     SGTLVTPEALEAAREATRQRAHSPGMVPIPESS"
     gene            complement(3629..4408)
                     /locus_tag="VM841_06330"
     CDS             complement(3629..4408)
                     /locus_tag="VM841_06330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL89837.1"
                     /translation="MSEATPLMYGELASWFHLLTPPKDYVETAASHRELFETLSSKPV
                     RTVLELGAGGGNNASHLKAWYQMTLTDRSPEMIATSRGLNPECEHVEGDMRSLRLGRT
                     FDAVLIDDAISYMTTEQDVLAALTTARAHLEPGGVVVFAPDETAEEYVPATEHGGEDG
                     EDRAMRYFSWDDVPVGTTVRTIYVYVLRDANGVRVEHEIHTFGLFPLATWETLIRRAG
                     FEPHRRSVEHWQHPQTKQYIFAGVAKSGTKKVRARDPRIAI"
     gene            complement(4405..4890)
                     /gene="paaD"
                     /locus_tag="VM841_06335"
     CDS             complement(4405..4890)
                     /gene="paaD"
                     /locus_tag="VM841_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HVL89838.1"
                     /translation="MVAATLEARVRAALEEVYDPEIPVVRITDLGIVEDVRASDAAIE
                     VDLLPTFTGCPALDAIREDVERAVAPLAEGREVRVTFRRDLAWTSDRISERAREAMKE
                     YGLAPPGERRGLTLVQIRVACPYCGSLDTAHDSTFGPTLCRDIRYCNACRNPFEGFKP
                     K"
     gene            complement(4884..5753)
                     /gene="paaC"
                     /locus_tag="VM841_06340"
     CDS             complement(4884..5753)
                     /gene="paaC"
                     /locus_tag="VM841_06340"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HVL89839.1"
                     /translation="MSEERMNLLLALADDELIIGHRMSEWTGWVPYVEEDLAFSSIAQ
                     DEMAHARLLYEIVSATGVDTLALGRQPHEYRNAVICERPNRDYAYTLARHFLYDTADA
                     VRVEALRASSWKELAEAFDVVTLEERYHLEHARTWFRRIANGPVEARAKLTTALQDVI
                     GEALSLFEPIPGEDALLADGTMPRASSEMLAAWLTQVGRELEEVGLERVLELAPEARI
                     GEMVPTSSGAIEAEGSSPTLRVPGLQRVDGQWVHVGEFEGAGGRSGRHSPDFAALWDE
                     MTGLYRAHPGATW"
     gene            complement(5750..6073)
                     /locus_tag="VM841_06345"
     CDS             complement(5750..6073)
                     /locus_tag="VM841_06345"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HVL89840.1"
                     /translation="MDVFEVFGRRQIDDPLVHVGAVKAADSEMALVLAREAYFRHGEG
                     VDCWVVRRGDMHRDPHPETLGGVTDKSYRRQDGYPGVGAKLRRVAEQLKDVRIDRPRP
                     KGAGS"
     gene            complement(6078..6995)
                     /gene="paaA"
                     /locus_tag="VM841_06350"
     CDS             complement(6078..6995)
                     /gene="paaA"
                     /locus_tag="VM841_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HVL89841.1"
                     /translation="MTYEERTAEFKARIDAGGKVEATDWMPDQYREACLKFIEMHANS
                     EIMGALPEREWIPRAPTLRRKLSLTAKVQDEVGHAQILYRIAEDLGKSREQMFDDLVN
                     GRTKFHNVFHYPTKSWGDVAVIGWLIDGAALVTQKALLDSSYAPYVRALRRICPEESL
                     HLRHGEDMVLELCGGSAEQRAMFQDAVNRWWAPIMHFFGPPSKADKDVLLYWGIKVRT
                     NEDLRQEFFSTYVPKLWDLGISVPDPELAYDEAAGQWRWGDPGWDEFWEVVRGNGPMT
                     ATRLTYRKAMWDSHAWLRDVFAGIGGAAA"
     gene            7074..7322
                     /locus_tag="VM841_06355"
     CDS             7074..7322
                     /locus_tag="VM841_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89842.1"
                     /translation="MRCEMCGNDYDKAFEVITAGERHVFDSFECAIHALAPVCEHCGC
                     KVVGHGVEVNGRFFCCAHCARETAGTSEVRDRVDIGAS"
     gene            complement(7385..8563)
                     /locus_tag="VM841_06360"
     CDS             complement(7385..8563)
                     /locus_tag="VM841_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89843.1"
                     /translation="MFETVGAINRAVGAVRDAASEATLRERPEVVAALLRGADALVAA
                     AAGLMAGGGVQAAAGRPVEHVLRTEARLTSWDASTVAGAADSLRAMPMLAAAFDEGLV
                     SWSQVRAILAAVKCVRAEDRPTIDALIGEQAKRLWDGSPDRLVDLVADEAARLRPDLS
                     QRREDRLIASSFLAIQGRLDGSAAIYGEADAVSAATIVGALDAVADAPVHPDRGVTRA
                     QQRFDALVQICETTLAGDGSGLRPRSRVLAVIDLKDVDRGGDGGATLLWPLPGRAPRL
                     TPVARDMLVCDAAVTPIVFNGRDVIGAADTHNTFSDRVRSAIVARDRRCRFCSHAAAQ
                     WCDVHHIVPGTGNTARDGILLCRRCHRIVHRHSWTATWHDDGAIRFERRGKHFLSLPP
                     "
     gene            8695..10206
                     /locus_tag="VM841_06365"
     CDS             8695..10206
                     /locus_tag="VM841_06365"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL89844.1"
                     /translation="MADYKLFINGEFVTAEDGKEFETIDPSTGQAHATVAQAGKADAE
                     KAIAAARAAFDDGRWSGLSPAKRAGFLTKIAAGIKERAGELANLETKDSGGTIRKTKG
                     EVAGASYTFRVLAEMATTVPLQETLPVTVAPGPSLNYLKREPLGVCAGIIPWNFPILM
                     AAWKIAPAIAAGNTVVLKPSENSPATALALAEIIKEAGVPDGVVNVIPGFGPSAGEPL
                     CNSMDVDKIAFTGSTAVGKRIMQLASSNIKKVTLELGGKSANVVLDDADLDLTVDGVL
                     WGIFYNSGQVCNAGSRLLVHQSLHDELVSRMVERARDMVVGDPADIGTDMGPIISQTQ
                     LDTIERYVQIGLDEGAKLAIGGERAPVAGKDGGYWYQPTIFTGVTNGMRIAQEEIFGP
                     VLSVIPFADNDEAVAIANDSIYGLAGGVWSRDPARAMGVADRMRTGTVWINDYHLINN
                     KYPFGGYKQSGLGREHGVQGFLEYTETKHVHVDTTGDRGKHRWFDTVVPRRKA"
     gene            10203..10964
                     /locus_tag="VM841_06370"
     CDS             10203..10964
                     /locus_tag="VM841_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVL89845.1"
                     /translation="MSFETELDAVASWCDETDRIALEVFREVIQPDVKPDGSLVTKAD
                     RDIESFIRLSIERSFPEDAVLGEEEGLSREGATRRWIVDPIDATTNYVRGIPIFATLI
                     ALEDEEGLALGFVSAPALHARWWATRGGGSFHNGTPIMVSDIATVNDAHIATGGMDLF
                     GPHELGDGIESVVERCDRHRGFGDFYGHMLVAQGSVEAMIDPIVSPWDLAACQLIVQE
                     AGGRFTSLQGHPGYDQGTALSSNGRVHDEILRLFA"
     gene            complement(10961..12127)
                     /locus_tag="VM841_06375"
     CDS             complement(10961..12127)
                     /locus_tag="VM841_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89846.1"
                     /translation="MGLRRVFAKPVAMALLTMLALAGAFVRVAAVGASEPAAGIGGNP
                     PVAAHADGPYLPPAGLRVPVGEEAGPAAPVASSAFAGMVVFVSGGDLWLDDLDDADEP
                     RLLIDNGDEQSSWSPRFVDRGNVSFVSEKGLEILDLTTGLRSVVAGNDPWIGAYDWSP
                     DGSRVAYMTSEWDEQRQVTRGYLRVVTRAGVAGEIPFSLIVPDGYCIHDPDGDFNVEF
                     SPDGRFILVENVAAQPAASVTVVAVTGETILEPSHLVWSGFDHDGTLVARELGTDDWV
                     RLSLTSGRVEQLPVPDKARRPALSPLGRSIAVDDGVGTVTLIDLAHGESSRPLRGYLN
                     PVWLDDERVIAFAVRACDQCEMPWEQTGDATLFDANFGERPLRIDFAQIDVLRR"
     gene            complement(12263..12682)
                     /locus_tag="VM841_06380"
     CDS             complement(12263..12682)
                     /locus_tag="VM841_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HVL89847.1"
                     /translation="MSECIFCAIAANQAPQFRVWDDEHAVAFMDINPAVEGHTLVIPR
                     RHARDLWDIEPADAQAVMAAAVTVGGMLKAALEPDGLNIVHATGRAAFQSVFHFHLHL
                     IPRRYGDPIRPPWSLEERNGDRTALAAVAERIRGAAG"
     gene            12757..13188
                     /locus_tag="VM841_06385"
     CDS             12757..13188
                     /locus_tag="VM841_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVL89848.1"
                     /translation="MKKSDIVEHLSTVPLFRELSSAHLAKIARVTDIKTARQGEALVR
                     EGTYRSGSGPAFFLIVDGTADVTIKRKKFATLKAGDTFGEMSLLDGQPRSATVTAKTD
                     MTLFRILGWHFTKLVKSEPGVALGLLKTMAQRLRAVEKKSF"
     gene            complement(13185..14144)
                     /locus_tag="VM841_06390"
     CDS             complement(13185..14144)
                     /locus_tag="VM841_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVL89849.1"
                     /translation="MSPLALTFLDGGRLWLLALLPLLIALYVGQQRRRRAYAMRFTNL
                     SLLAAVAPKRPGWKRHVSAAMILVALMLLGGAFARPATDVRVPRGRAAVVLVVDVSRS
                     MRATDLSPNRMAAAKTASLAFVDSVPGEMELAVVSFSNAAALLAPITRDRAVVRGAID
                     RLEPIAGTAMGEGLAVALDEIARARQAEGQAIPASVLLLSDGVSNLGRPPEEAADTAT
                     EMDVPVYTVGVGTEGATLEFNGQLIPVDVDEVTLRAVAEATGGEYFRSGDTKTLAAVY
                     KNLGSVLGYDLEQREVTAAVAGLAGLILLGAAIAGTLLTNRIP"
     gene            complement(14141..15067)
                     /locus_tag="VM841_06395"
     CDS             complement(14141..15067)
                     /locus_tag="VM841_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HVL89850.1"
                     /translation="MRHLSAVAAVADDPVVVPGADRLLRGADLWLARRVDGHGPGRHR
                     SIRLGEGLELADIREYTPGDDVRTIDWNVTARTGRPHVRVYDADRDVTVLVVADRSAS
                     MGFGTANATKDRLARELVAAVAAMVVRRGDRIGGLLFDEERAGGMRAASGRRAALRLL
                     HAMEAAPPKDRTAARMDLALTDAGRMAKRRSVVLAISDWIDAGAWEEPLRLLAYRHEV
                     IAAEIRDPREDTLPDVGTVVLEDPETGRQLEVDTGIAGLREAFETAAAQQRAARAASF
                     ARARASHLIVSTDRDWRADLIKYLTLRKKGRR"
     gene            complement(15064..>15757)
                     /locus_tag="VM841_06400"
     CDS             complement(15064..>15757)
                     /locus_tag="VM841_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVL89851.1"
                     /translation="FTNFLLADEINRAPAKVQSALLEVMQEHQVTIGGQSYAVPEPFI
                     ALATQNPIESEGTYPLPEAQMDRFMMKVLVDYPDHGEELAVVERAEHALEPPRQILEP
                     GTLFALQSLTQQVFVDHRIREYVVTVVSATRRPDDYGVGEIGPYVEYGASPRGSINMI
                     LAARAIAVQHGRDYVLPADVDAILMDVLRHRVIPSYRALAEDVTADQIVARVREKIAP
                     PEIDINEAAQGA"
BASE COUNT         2643 a         5518 c         5243 g         2353 t
ORIGIN      
        1 ggcgccggca gcggctcgaa ctttcccttc tatccgccgg aggtcacgca ggtggccgcg
       61 gtcgagccgg agccgtatct gcgcggccga gccgaagaag cggcgaagaa ggcgccggtt
      121 ccggtgacgg tgcatgcggc caccgccgcg tcgctcccct tccccgacgc ctcgttcgac
      181 gccggcgtgt tctgcttggt tctgtgcagc gtcccctcgc aacccgaggc actggccgag
      241 atgtatcgcg tcctcaagcc gggaggcgag ctgcgcttct acgagcacgt cgccgccgcg
      301 ccgggcaaga tgctccgccg cgtccagcaa gcatccgaca cggtctggcc gcgtctgttc
      361 ggcggatgcc acacccgccg cgacaccgtg tccgcgatcc gggacgccgg attcgaaatc
      421 gatcgcgtca aagacctgtc gctcacgatc gccggtgtgc cgtccccgat caccccgcac
      481 gccgccggcg tcgcgcgccg cccgcaagcg agcaaaccgg ccgccgccga ataaaccttc
      541 gccgtcccgg gtatgaacac gacgagcgaa aggagcgcat cgccatgtct accctgatgt
      601 cttcgatcaa cctggatgtg cccgtgcaaa ccgcgtacaa ccagtggacg caattcgagg
      661 agttcccgaa gttcatggac ggcatcgagt cggtgacgca gatcgacgac gtgacgctgc
      721 gctgggtcgc caaaatcgac ggcgttcaac gcgaatggac cagcgagatc gtcgagcaga
      781 agcccgacga catcatcgcg tggatcaacc acgaaggccc caacacggcc ggcagcgtca
      841 agttcgagcc gtcggcgagc gggggctgca aagtcgaggt cacgatgaaa tacgaccccg
      901 acaccttcac cgagaaggcc gccgagacac tcggaatcat tcagcgcagg ctggacggcg
      961 acctgcgccg cttcaaagac ttcatcgaag agcgcggccg cgaaacaggc gcatggcgcg
     1021 gcgaggtcca caccgcccaa gtcgagtaga cgcagcgttc aacgcacgca aggaggacgc
     1081 ctcgaaagcg gcgtcctctt tcgtgtcgtc aagccgccag cgacgcttcg tccaggatcc
     1141 aaccacggct gcgcacattt cgcacgaagt tgcggccgag gctatggcgg accgtggtca
     1201 gcagcacgtc caccgaccgt gcgtgacgca gccccagcga gcgagcgatc tcctcgcgcg
     1261 cgaccacgcg atcgcggttg gcgatgagca gcgccaccag ctcgacttgt gtcgaggtca
     1321 acgccacctc gttgcccccg aaccagaccc gatacgcggc cggctcgacg atcagcgctc
     1381 ccgcttccag cagcacctcc acgtgcgccc gcttgttggt gtccgggctc atcggcggcg
     1441 aaccgagcgg cggacgcgct tgccggcgaa cgccgctctc aatgcgcgct tgtccgccgc
     1501 tgtcagcgcg gcgccggtac ggcccgtgac ttcgaacacg tcgatcgctt cggtgccgat
     1561 cgtggtgatc gtcgcctggt ggatctcgac acctgctcgc gagatcgcgg ccgcgacctt
     1621 ggcgagcagc ccgacacgat cttcggcgcg cacctcgatg atggaccgag cgcccgtccc
     1681 atcgggcgcg atcgccaggg tcgcgtcaac ggcttcggat ggcgtccggc catagccggc
     1741 gacgaggccg gcatcgatcc gggatcgcac gcggtcccag tcggtccatt ccgaggcatc
     1801 aacgcggaac acgtcgatga cgactccgcc gggtccggtg gcgatgtgcg cgctgcagac
     1861 cgcgatgccg gcgccggcga acgacgaggc gatcgcggcg agcgcgccgc gacggtcgcg
     1921 cagcgcgact cgcacggtcc attgctcggc tccttcgtga tcggcgacca agcgcgtctg
     1981 gtacagcccg aggttcgcag ccgattcgcc gtcgattgtt cgtgccggtt ccacagcctc
     2041 ctcctttcgt gtgacgcgct agctcgattc ggggatcggt accatgccgg gcgagtgggc
     2101 ccgctgccgc gtggcttcgc gcgccgcctc cagcgcttcc ggcgtgacga gcgtgccgct
     2161 gcccaggtgc tcctccgggt agccccacat gccgtgctcg ccgatgtcga gcccttccat
     2221 ctcttcctcg gcgctcacgc gcaagccgac cgtcgctttg accagaccga acacgacggc
     2281 cgatgcgacc agcacccagc ccatgacggc ggccgcgccg agcgcctgca cgccgagctg
     2341 agccatgccg ccgccgtaga cgagccccgg cgagccgacg ccggccgcgt cgatgagccg
     2401 cggcgaggcg aagaccccca ccatgagggt gccgagcacg ccgcacacgc cgtggaccga
     2461 caccgcgccg acggggtcgt cgaccttgac gcggtcgaag aacatgatcg cgccgacgac
     2521 caccacaccg gcgatcgcgc cgatgacgag cgccgcccag ttctcgacga acgcgcagcc
     2581 cgcggtgatg gcgacgagcc ctgcgagcgc gccgttggcg ctcatcccga cgtcgggctt
     2641 gcgcccgagc atccaggacg cggtcatcgc catgatcgcc cccgccgccg cggccaggtt
     2701 ggtcgtgacc gcgatgtcgg cgatcgaaag cgtcgcggcc atcgtcgagc cggggttgaa
     2761 gccgaaccat ccgaaccaga gcaggaagac gccgagcatg ccgagcggca tcgagtggcc
     2821 cggcatcggc cgcggccgtc cgtctttcgc gtacttgccg agccgcggcc cgaccatgat
     2881 cgcgccgacc agcgccgccc atccgcccac cgaatgcacg accgtcgatc cggcgaagtc
     2941 gaggaactcc ttcgaggcga gccagccgcc gccccagatc cagtggcccg cgatcgggta
     3001 gatgagcccc gagatcaggc cgctatagag aaggtatgcc ttgaacttgg tgcgttcggc
     3061 catcgcgccc gacacgatcg tcgccgcggt tcccgcgaag acgacctgga agagccactt
     3121 cgccatcagc gggggcgtgg cccagtcgag cgaagagaag gcggtcggat cggacgagaa
     3181 ccatcccgac gtgccgatga gcgcgttgcc ggtgccgaac atcagcgcga acccgacggc
     3241 ccagaacgcg atcgagctca tcccgaagtc catcaggttc ttcatcacga tgttgcccgc
     3301 gttcttcttg cgagtgaacc cggcctcgac catcgcgaac ccgccctgca tgaggaacac
     3361 catgaacgcc gccatcagcg tccacaccgt gtcgagcagg atcttctcct ccgagggccc
     3421 gtcggccgtc tgcgcgaacg ccgccgtggc cgtgacgatc agaaacaccg ccgtcagagc
     3481 accgacggta agaattctcc gcttccgcat gccacctccc aaagctcgtc ccatcagagc
     3541 cgatggtggc agacgcagat ttcaaagccg tatccgcgga gtcacggcca ggtaaaagtc
     3601 gcgttacgcc gatgtttcgg accctggatc agattgcgat gcgggggtca cgtgcacgca
     3661 ccttcttagt cccactcttc gcgacacccg cgaagatgta ttgcttcgtc tggggatgct
     3721 gccagtgctc gacggagcgg cggtgaggtt cgaagccggc gcggcggatg agcgtctccc
     3781 aggtggcgag cggaaagagg ccgaaggtgt ggatctcgtg ctcgacgcgc acgccgttcg
     3841 cgtcccgcag gacgtacacg tagatcgtcc gcacggtggt gccgacgggc acgtcgtccc
     3901 atgagaagta gcgcatcgcg cgatcctcgc cgtcctcgcc gccgtgctcg gtagccggca
     3961 cgtactcctc ggcggtctcg tcgggcgcga agacgacgac gccgcccggc tccagatgcg
     4021 cccgcgcggt agtgagcgcg gcgagcacat cctgttcggt cgtcatgtag gagatcgcgt
     4081 cgtcgatcag cacggcatcg aaggttcgtc ccagccgcag actgcgcatg tcgccctcga
     4141 cgtgctcgca ctcggggttc aggccgcgag acgtcgcgat catctcgggc gagcggtcgg
     4201 tgagcgtcat ctggtaccac gccttcagat gcgaagcgtt gttgccgccg cccgccccca
     4261 gctcgagcac ggtccggacc ggcttggacg atagcgtctc gaacagctcg cgatgggacg
     4321 cggcggtctc gacgtaatct ttgggcgggg tgagcagatg gaaccaggat gccagctcgc
     4381 cgtacatcag cggcgttgcc tcgctcattt cggcttgaag ccttcgaacg ggttgcggca
     4441 cgcgttgcag tagcggatgt cgcggcacaa ggtcggcccg aacgtcgagt cgtgcgcagt
     4501 gtccagcgat ccgcagtacg gacacgcgac cctgatctgc accagcgtca ggccgcggcg
     4561 ctcgccgggc ggcgccagcc cgtattcctt catcgcctcg cgcgcccgct cgctgatccg
     4621 gtccgacgtc caggcgaggt cgcggcggaa cgtgacgcgc acctcgcggc cttcggcgag
     4681 cggcgcgacc gcacgctcga cgtcttcgcg gatggcgtcg agagccggac agccggtgaa
     4741 ggtcggcagc agatccacct cgatcgcggc gtcgctcgct ctcacgtctt cgacgatgcc
     4801 gagatcggtg atccgcacga ccggaatctc ggggtcgtag acctcctcca gcgccgcgcg
     4861 gacgcgcgct tcgagcgtcg cggctaccac gtcgcccccg gatgcgcgcg atacagcccg
     4921 gtcatctcgt cccagagcgc ggcgaagtcg ggtgagtggc ggccggagcg gccgccggcg
     4981 ccttcgaact cgccgacgtg cacccattgt ccgtccactc gctgcaagcc ggggacgcga
     5041 agggtcggcg aggatccttc cgcctcgatc gcgcccgacg atgtcggaac catctcgccg
     5101 atccgcgctt cgggagccag ctcgagcact cgttccaaac cgacctcttc gagttcgcgc
     5161 cccacctggg tcagccacgc tgcgagcatc tcggagctgg cgcgcggcat ggtgccgtcg
     5221 gcgagcagcg catcttcgcc ggggatcggc tcgaacagcg acagcgcctc cccgatcacg
     5281 tcctgcaatg cggtggtcag cttggcgcgc gcctcgacgg gtccgttcgc gatgcgccgg
     5341 aaccaggtgc gcgcatgctc gaggtgatag cgctcctcga gcgtgacgac gtcgaacgct
     5401 tccgcaagct ccttccacga cgacgcgcgc agcgcctcga cgcgaaccgc atcggcggtg
     5461 tcgtagagga agtgccgcgc gagcgtgtag gcgtagtcgc ggttgggccg ctcgcagatg
     5521 acggcgttgc gatactcgtg cggctggcgg cccaacgcga gcgtatccac gccggtcgcc
     5581 gagacgatct cgtagagcag acgcgcgtgg gccatctcgt cctgcgcgat cgaggagaac
     5641 gccagatcct cttcgacgta gggcacccag ccggtccatt cgctcatgcg atggccgatg
     5701 atcagctcgt cgtcggcgag cgccagaagc aggttcatcc gctcctcgct cacgatcccg
     5761 ctcccttggg ccgcggccgg tcgatgcgca catccttgag ctgttcggcg acgcggcgca
     5821 gcttcgcgcc gacgcccgga tagccgtcct ggcgccgata ggacttgtcg gtgacgccgc
     5881 cgagcgtttc gggatgcggg tcgcggtgca tgtcgccgcg ccgcacgacc cagcagtcca
     5941 cgccctcgcc gtgccggaag tacgcctcgc gcgccagcac caaagccatc tccgagtccg
     6001 cggccttgac cgcgccgacg tgaacgagcg gatcgtcgat ctgccggcgg ccgaatacct
     6061 cgaagacgtc cacggggcta agccgcagcg ccgccgatgc cggcgaacac gtcgcgcagc
     6121 caggcgtgcg aatcccacat cgccttgcgg taggtcagcc gcgtcgcggt catcggaccg
     6181 ttgccgcgca cgacctccca gaactcgtcc cagccgggat cgccccagcg ccactgcccg
     6241 gccgcctcgt cgtaggcaag ctcgggatcg ggaacgctga tgcccagatc ccacagcttc
     6301 ggcacgtagg tgctgaagaa ctcttggcgc aggtcttcgt tcgtgcggac cttgatgccc
     6361 caatacagaa ggacgtcctt gtcggccttg gacggcggac cgaaaaagtg catgatcggc
     6421 gcccaccagc ggttgaccgc gtcctggaac atcgcgcgct gctcggcgct gccgccgcac
     6481 agctcgagca ccatgtcctc gccgtggcgt aggtgcagcg actcctcggg gcagatgcgt
     6541 cgcagcgcgc gcacgtacgg agcgtaggac gagtcgagca gcgccttctg cgtgaccagc
     6601 gccgcaccgt cgatcagcca gccgatgacc gcgacgtcgc cccaggactt ggtcggatag
     6661 tggaagacgt tgtggaactt ggtgcggccg ttgaccaggt cgtcgaacat ctgctcgcgg
     6721 cttttgccga ggtcttcggc gatccggtac aggatctgcg cgtggccgac ctcgtcctgc
     6781 accttcgcgg tcagcgagag cttgcggcga agggttgggg cgcgcggaat ccattcgcgt
     6841 tcgggcagcg cccccatgat ctcgctgttg gcatgcatct cgatgaactt caggcacgcc
     6901 tcgcgatact ggtcgggcat ccagtcggtc gcctcgacct tgccgcccgc atcgatgcga
     6961 gccttgaact cggccgtccg ctcctcgtag gtcatccacc catcttagcg cctccctcca
     7021 gggcgtttga ccctcggccg gcggggggag gccacaacgg gaaggaggtc gccatgcgct
     7081 gcgagatgtg cggaaacgac tacgacaagg cattcgaggt gatcaccgcc ggcgagcgac
     7141 acgtctttga ttcgttcgag tgcgcgatcc acgctctggc gccggtctgc gagcactgcg
     7201 gctgcaaggt cgtcggccac ggagtcgagg tgaacggccg cttcttctgc tgcgctcact
     7261 gcgcccgaga gacggccggc acgagcgagg ttcgcgaccg agtggacatc ggcgcctcct
     7321 gagccgggct tcgggcacgc cgaagtggcc gcgagagggc ggctggtgcg agaaccgtcg
     7381 caggctacgg cgggaggctg aggaagtgtt tgccgcggcg ctcgaatcgg atggcgccgt
     7441 cgtcgtgcca ggtggcggtc cagctatggc gatggacgat ccggtggcat cgccggcaga
     7501 gcaagatccc gtcgcgagcg gtgttgcctg tgcccggcac gatgtggtgg acgtcacacc
     7561 attgggcggc ggcgtggctg cagaagcggc agcgccgatc ccgcgccaca atcgcgcttc
     7621 gtaccctgtc ggagaacgtg ttgtgggtat ctgcggcgcc gatcacgtcg cggccgttga
     7681 acacgatcgg ggtgacggcc gcgtcgcaga cgagcatgtc gcgagccacc ggggtcagcc
     7741 gcggggcgcg gccgggcagc ggccacagca gcgtggcgcc cccgtcgccg ccgcggtcaa
     7801 catccttgag atcgatcacg gccagcacac gcgagcgggg tcgaaggccg ctcccgtcac
     7861 ccgcaagcgt cgtctcacag atctgaacga gcgcatcaaa gcgttgctgc gcgcgagtga
     7921 cgccgcggtc cggatgcacc ggcgcgtctg ccaccgcatc caaggcaccc acgatcgtcg
     7981 cggccgaaac cgcgtcggct tcgccgtaga tcgcggccga gccgtccagg cggccctgga
     8041 tcgccagaaa actcgacgcg atcaagcgat cctcgcgacg ctgcgacaga tccggccgca
     8101 aacgcgcggc ctcatctgca accagatcca ccagccgatc gggactgcca tcccagagcc
     8161 gtttagcctg ctcgccgatc agcgcatcga ttgtcggacg atcctcggcc cggacgcact
     8221 tgaccgcagc caagatcgcc cgcacctgac tccacgagac cagaccctcg tcgaacgcgg
     8281 ctgccaacat gggcatcgcg cgaagcgaat cagccgcccc cgcaaccgtg gacgcatccc
     8341 acgaggtcag ccgcgcctca gtacgaagca catgctccac cggccggccc gccgccgcct
     8401 gaaccccgcc gccggccatc agacccgccg ccgccgcgac caacgcatcc gcaccacgca
     8461 gcagcgccgc aaccacctcg ggccgctccc gcagcgtcgc ctcggacgcc gcgtcgcgca
     8521 ccgcgccgac tgcgcgattg atggctccga cggtttcgaa catgtgttcg agcctatcgc
     8581 agcctgctca caattgcaag caattttctt gccattgcgg gaaagacatg acgacggcgg
     8641 gaagcatcgg ttcgctagca tgacgtccgg atcacgagtc cgggaggaac ccacatggcc
     8701 gactacaagc ttttcatcaa cggcgagttc gtcaccgccg aggacggcaa ggaattcgaa
     8761 acgatcgacc cgtccaccgg ccaggcgcac gccaccgtcg cccaagccgg caaggcggac
     8821 gcggaaaaag ccatcgccgc cgcgcgtgcc gcgttcgacg acggacgctg gagcgggctg
     8881 tcccccgcca agcgcgccgg cttcctgacc aagatcgccg ccgggatcaa ggagcgcgcc
     8941 ggcgagctcg caaatctgga gaccaaggat tcgggcggca cgatccgcaa gaccaaaggc
     9001 gaggtcgcgg gcgcgtccta cacgttccgc gtcctggccg aaatggccac gaccgtcccg
     9061 ctgcaggaga ccctgccggt caccgtcgca cccggcccgt cgctgaacta tctcaagcgc
     9121 gaaccgctgg gcgtgtgcgc gggcatcatc ccgtggaact tcccgatcct gatggccgcg
     9181 tggaagatcg cacccgcgat cgcggccggc aacacggtgg tcctcaagcc gagcgagaac
     9241 tcccccgcga ccgcgctggc gctggccgag atcatcaaag aggccggcgt gcccgacggc
     9301 gtggtcaacg tgatccccgg gttcggcccg tcggcgggcg agccgctgtg caacagcatg
     9361 gacgtggaca agatcgcatt caccggctcg accgcggtcg gaaagcgcat catgcagctc
     9421 gcgtcgagca acatcaagaa ggtcacgctg gagctgggcg gcaagagcgc gaacgtcgtg
     9481 ctcgacgacg ccgatctgga tctgacggtg gacggggtcc tgtggggcat cttctacaac
     9541 tcgggtcagg tctgcaacgc cggatcgcgg ctgctggtgc accagtcgct gcacgacgag
     9601 ctggtttcgc gcatggtcga gcgcgcccgc gacatggtcg taggcgatcc ggccgacata
     9661 ggaaccgaca tggggccgat catctcgcag acccagctcg acacgatcga gcggtacgtg
     9721 cagattggtt tggacgaggg cgcgaagctg gcgatcggcg gcgagcgcgc acccgtggcg
     9781 ggcaaggacg gcggctactg gtaccagccg acgatcttca ccggcgtcac caacggtatg
     9841 cgcatcgcac aggaagagat cttcgggccc gtgctgtcgg tgatcccgtt cgccgacaac
     9901 gacgaggccg tggcgatcgc gaacgactcc atctacggcc tggcgggcgg ggtgtggtcg
     9961 cgcgatccgg cgcgcgcgat gggcgtggcc gaccggatgc ggaccggtac ggtctggatc
    10021 aacgactacc acctgatcaa caacaagtat ccgttcggcg gatacaagca gtccggcctg
    10081 ggccgcgagc acggcgtgca aggcttcctc gagtacaccg agaccaagca cgtgcacgtg
    10141 gacacgaccg gcgaccgcgg caagcaccgc tggttcgaca ccgtcgttcc gcgcaggaag
    10201 gcgtgagctt cgaaaccgaa ctggacgccg tcgcatcctg gtgcgacgaa acggaccgca
    10261 tcgcgctgga agtctttcgc gaggtgatcc agcccgacgt caagccggac ggctcgctgg
    10321 tgacgaaagc cgaccgcgac atcgaatcgt tcatccgcct ctcgatcgag cggagcttcc
    10381 ccgaagacgc ggtcctcggc gaagaagaag gcttgtcacg cgaaggcgcg acgcggcggt
    10441 ggatcgtcga tccgatcgac gccaccacca actacgtgcg cggcatcccg atcttcgcga
    10501 cgctgatcgc gctggaggac gaggagggcc tggcgctggg attcgtcagc gcgccggcgc
    10561 tgcacgcccg ctggtgggcg acccgcggcg gcggctcgtt ccacaacggc accccgatca
    10621 tggtctccga catcgcgacc gtcaacgacg cgcacatcgc aaccggcggc atggacctgt
    10681 tcggcccgca cgagctcgga gacggcatcg agtccgtcgt cgagcgatgc gaccggcatc
    10741 gcggcttcgg cgacttctac ggccacatgc tcgtcgcgca aggatcggtc gaggcgatga
    10801 tcgatccgat cgtctcgcca tgggatctgg ccgcctgcca gctcatcgtg caagaggccg
    10861 gcggccggtt cacgtccctg cagggccatc ccggctacga ccagggcacc gcgctgtcga
    10921 gcaacggccg cgtgcacgac gagatcctga ggctgttcgc ctagcgacgc agaacatcga
    10981 tctgcgcgaa gtcgatgcgc agcggacgct cgccgaagtt cgcgtcgaac agcgtcgcgt
    11041 cgccggtctg ctcccacggc atctcgcatt gatcgcaggc acgcaccgcg aacgcgatca
    11101 cgcgctcgtc gtcgagccag accggattta gataaccgcg cagaggccgc gacgactcgc
    11161 cgtgcgcgag atcgatcagc gtgacggtgc cgacgccgtc gtcgacggcg atcgagcggc
    11221 cgagcggcga cagagcaggg cgacgggcct tgtccggcac cggaagctgt tccacgcggc
    11281 ccgatgtcag cgacaaccgg acccaatcgt cggttcccag ttcgcgtgcg accagcgtgc
    11341 cgtcgtgatc aaagcctgac cacaccagat gcgacggctc caggatcgtc tcgccggtca
    11401 cggccacgac cgtcaccgac gcggccggct gcgcggctac gttctcgacc aggatgaagc
    11461 ggccgtcggg cgagaactcg acgttgaagt ctccgtccgg atcgtgaatg cagtagccgt
    11521 cgggcacgat cagcgagaac ggaatctcgc cggcgacgcc cgctcgggtc acaacacgca
    11581 gatagccgcg ggtcacctgc cgttgctcgt cccattcgct ggtcatgtac gcgacccgcg
    11641 agccgtcggg actccaatcg taggcgccga tccacggatc gttgccggca acgaccgagc
    11701 gcagaccggt ggtcagatcc agaatctcca atcccttctc ggaaacgaac gaaacgtttc
    11761 cgcgatcgac gaaccgcggc gaccacgagg actgctcgtc gccgttgtcg atcaacagcc
    11821 gcggctcgtc ggcgtcgtcg agatcgtcga gccacaaatc cccgccggaa acgaagacga
    11881 ccatgcccgc aaaggcggac gaggcgaccg gcgcggccgg gccggcctcc tcgcccacgg
    11941 gaacacgcag accggccggg gggaggtacg gcccgtccgc gtgcgcagcg accggcgggt
    12001 tgccaccgat tcccgccgca ggctcggagg cccccaccgc ggcgacccgc acgaacgcgc
    12061 cggccaaagc gagcatcgtc agcagcgcca tggcaaccgg ctttgcgaag actctccgaa
    12121 gacccatcgc gtcctgcctt tcgcgagggc ggcccgggtg gccgctctcc tctgcagcga
    12181 tgatgcgtcc ggcgaccccg gccccgatac cgaccagcgg ccatttcggc gggccgctag
    12241 gacactgtcc gaacgggcag gcctacccgg cagcgccgcg gatccgctcg gcgaccgcgg
    12301 ccaaggcggt ccggtcgcca ttgcgctcct ccagcgacca gggcggccga atcggatcgc
    12361 cgtagcgacg cgggatcagg tgcagatgaa agtgaaagac gctctggaag gcggcccggc
    12421 cggtcgcgtg gacgatgttc agcccgtcgg gctccagtgc cgccttcagc atcccgccga
    12481 ccgtcacggc ggcggccatc accgcctgcg cgtcggccgg ctcgatgtcc cacagatctc
    12541 tggcgtgccg ccgcggaatg accagcgtgt ggccttcgac ggccggattg atgtccatga
    12601 acgcgaccgc atgctcgtca tcccacacgc gaaactgcgg ggcttgattc gccgcgatgg
    12661 cgcagaagat gcattcggac atgcacggca gtctagcggc ttgttttggc gccgagcctc
    12721 ccactatcct gaccgcgtcc agggggagga tacccagtga agaaatcaga tatcgtcgag
    12781 cacttgtcca cggtgccgct gttccgcgag ctcagcagcg cgcacctggc gaagatcgcg
    12841 cgcgtcaccg acatcaagac cgcccgccag ggcgaggcgc tggttcgcga aggcacctac
    12901 cgctccggct cgggcccggc gttcttcctg atcgtggacg gcacggccga cgtcaccatc
    12961 aagcgcaaga agttcgcgac gctcaaggcc ggagacacct tcggcgagat gtcgctgctg
    13021 gacggccagc cgcgctcggc gacggtcacg gccaagaccg acatgacgct gtttcgcatc
    13081 ttgggctggc acttcaccaa gctcgtcaag agcgagccgg gtgttgcgct gggactgctc
    13141 aagacgatgg cgcaacgcct ccgcgccgtc gaaaaaaagt ccttctaagg aatccggttc
    13201 gtcagcaggg tgccggcgat cgcggcgccg agcagaatga ggccggcgag ccctgcgacg
    13261 gctgccgtga cctctcgctg ctccaggtcg tagcctagga ccgagccgag gtttttgtag
    13321 acggcggcga gggttttggt gtcgcccgag cggaagtact cccctccggt cgcctcggcc
    13381 accgcgcgta gcgtgacctc gtccacgtcc accgggatca actggccgtt gaactcgagc
    13441 gtcgcgcctt cggtgccgac gcccacggtg tagacgggga cgtccatctc ggtggcggtg
    13501 tcggcggcct cctcgggcgg acgacccaga ttcgacacgc cgtccgacag cagcagcacc
    13561 gaggcgggga tggcttgtcc ttcggcctgg cgcgcgcggg cgatctcgtc gagcgcgacc
    13621 gccagacctt cgcccatggc ggtgccggcg atcggctcca agcggtcgat ggcgccgcgc
    13681 accacggcgc ggtcgcgcgt gatcggcgcg agcagcgcgg ccgcgttgct gaacgacacc
    13741 accgccagct ccatctcgcc gggcaccgag tccacgaacg cgagcgaggc ggtcttcgcc
    13801 gcggccatcc ggttgggcga aagatcggtg gcgcgcatcg accgtgacac gtccacgacc
    13861 agcacaacgg cggctcggcc gcgcggtacg cgcacgtccg tcgccggacg cgcgaaggcc
    13921 ccgcccagaa gcatcagcgc gacgaggatc atcgcggccg acacgtgccg tttccagccg
    13981 ggccgtttcg gtgcgaccgc cgcgagcagc gacaggttcg tgaagcgcat cgcgtaggcg
    14041 cggcggcggc gctgctgccc gacgtagagc gcgatcagca gcgggagcag cgccagcagc
    14101 cataggcgcc cgccgtccag aaacgtcagc gcgagcgggc tcaccggcgg cccttcttgc
    14161 gcagcgtgag gtacttgatc aggtcggcgc gccagtcgcg gtcggtggac acgatcaggt
    14221 gcgaggcgcg ggcgcgcgcg aacgacgccg cgcgggcggc gcgctgctgc gcggcggccg
    14281 tctcgaacgc ctcacgcagg ccggcgattc cggtgtcgac ctcgagctga cgtccggtct
    14341 cgggatcttc gagcacgacc gtgccgacat cgggcaacgt gtcctcgcgc ggatcgcgga
    14401 tctcggccgc gatcacctcg tggcggtagg ccaggagccg cagcggctcc tcccaggcgc
    14461 ccgcatcgat ccagtcgctg atcgcgagca cgacgctgcg gcgtttcgcc atgcggccgg
    14521 cgtcggtaag cgcgagatcc atgcgtgcgg cggtccgatc cttgggcggc gcggcttcca
    14581 tcgcgtgcag cagccgaagc gccgcgcgcc ggccggatgc ggcgcgcatg ccgccggcgc
    14641 gctcctcgtc gaacagcagc ccgccgatcc ggtcgccgcg gcgcacgacc atcgccgcga
    14701 ccgcggcgac gagctcgcgg gcgagccggt ccttggtcgc gttcgcggtg ccgaacccca
    14761 tcgaggcgct ccggtcggcg acgaccagca ccgtgacgtc gcggtcggcg tcatagacgc
    14821 gcacgtgcgg ccgtccggtg cgcgcggtga cgttccagtc gatggtgcgc acgtcgtcgc
    14881 ccggcgtgta ttcgcggatg tcggcgagct cgagcccttc gccgagccgg atgctgcgat
    14941 gccgtccggg gccgtgcccg tccacgcgac gcgccagcca caggtcggcg ccgcgcaaca
    15001 gccggtccgc gccgggtacg acgaccgggt cgtcggcgac ggcggccacc gccgacagat
    15061 gcctcatgcg ccttgcgccg cctcgttgat gtcgatctca ggcggcgcga tcttctcgcg
    15121 aacacgcgcc acgatctgat cggcggtgac gtcctcggcc aaggcccggt aagacgggat
    15181 gacgcggtgg cggagcacgt ccatcaggat cgcgtccaca tcggcgggca gcacgtagtc
    15241 gcggccgtgc tgtaccgcga tcgcgcgcgc cgccaggatc atgttgatcg agccgcgcgg
    15301 gctcgccccg tactcgacgt aagggccgat ctcgccgacc ccgtagtcgt cgggccgccg
    15361 cgtcgccgac acgaccgtca ccacgtactc gcggatgcgg tggtccacga acacctgctg
    15421 cgtgagcgac tgcaacgcga acagcgtgcc cggctcaagg atctggcgcg gcggctcgag
    15481 cgcgtgctcg gcgcgctcga cgaccgcgag ctcctcgccg tgatcgggat agtccacgag
    15541 caccttcatc atgaagcggt ccatctgcgc ctcgggcagc ggataggttc cttcggactc
    15601 gatcgggttc tgcgtggcga gcgcgatgaa cggctccggc accgcgtagc tttggccgcc
    15661 gatggtgacc tgatgctctt gcatcacctc gagcagcgcg ctttgcacct tcgcgggcgc
    15721 gcggttgatc tcgtcggcga gcaggaagtt ggtgaac
//
LOCUS       DATLFO010000165        27885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_3469, whole genome shotgun sequence.
ACCESSION   DATLFO010000165 DATLFO010000000
VERSION     DATLFO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27885
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3469"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VM841_06405"
     CDS             complement(<1..456)
                     /locus_tag="VM841_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89852.1"
                     /translation="MPTSGGNTTKHAAHPARTYIAVTMLIMLVASPATAFGTKDGPVR
                     SAVCDIRALAGPLLGSCRSSEPEPGPEPSAEPRASSQPDERPGERPSANAPPRAAASG
                     TTGAIPPLRIEDPAPAGHGGATADAGESPSGLPAGAPAGSPDGDSPASAV"
     gene            complement(573..2429)
                     /locus_tag="VM841_06410"
     CDS             complement(573..2429)
                     /locus_tag="VM841_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL89853.1"
                     /translation="MSSTRVLRALLAIACVVPVVAPRVAPAASSPTAFRAGEALVRFA
                     PGTPSALIAAAHARAGARVISSIPVIGYQRVAFDPSLPFDAVLASYRNDPTVAVAEPN
                     HLARLAAEPDDPCVTGGCATAGQWALRMTNPSWESFPSAQQLRSMERVAVAVLDTKID
                     AAHPDFANRGSTSTDARDGGQLVMADARDWIPASQQNGAAHYHGTYVAGLVAAATDNG
                     TGIAALGHTAAILPLTVVDGAGTADAASIADAIVYAWQRGARVINLSLGLTADSAAVR
                     DAIRTAAGGTSPALIVAAAGNNTGSAPFYPGSYPDVMSVAGTNDADSYASCSNHNANV
                     SVAAPAQRVVSLNVGGGTVAPPCGTSAAAPQVSGLAALLFAQDPSRTPAQVRAIIERS
                     ADDLGPPGRDDRFGHGRINAERALRDGPAATNARSTVPGPAGTTTITASAAAPAGVKA
                     ARVFIGSPSSDAIALSAADGAFGQAAETLRATVAVPTGLAAGPHPIYVQAFDGSRWGP
                     KTVGVLMIDGQAPVIASAAASHGVRATGQAVVVTASLGDDASSSLSVGVQVFDAQNRV
                     VFQEARTGVPLGGFSYSWTPAGSVLPGTYQVKIIAVDQSGKSSAVLVGTIVA"
     gene            2590..3237
                     /locus_tag="VM841_06415"
     CDS             2590..3237
                     /locus_tag="VM841_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89854.1"
                     /translation="MNRPRLSLAAYPAAFVVGAILGTFWDRLHVVAGTLVYADEGVFG
                     QPFWIPLEFGAAAVAGVMVFTLLGDPAPRRESPRVAAIETLWISAVYAATAFFDRWPW
                     ALAALLLILLAVRAKDLAPIVAGSPIPSLALLLGGPLFEAALISSGVYSYRNSQLGPL
                     PVWLPVLWAHGVLFMMRFSEAMLLRFGVRRRIQPATATLTPTDPEPDPEPDPIED"
     gene            3240..4409
                     /locus_tag="VM841_06420"
     CDS             3240..4409
                     /locus_tag="VM841_06420"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HVL89855.1"
                     /translation="MRVLCAAVTGPGHVYPVMAAARALAARGHEPVLAFESRFEHLAE
                     REGMAFVPLPGVRSADPDAFKPYEISLAIARAFAPEVRRIRPDAALVDIITHGVALAC
                     EAAGVPFATLCPHPLPYPSKDLAPFGAARPPRRGAWGRRRDDRARARHLADLERGRDE
                     CNAIRGAMGLPALDRLDVAWSREAILVATPAALEPPRSDWPAHARVVGPCLYEPDGTA
                     PAIPPGDGPLVLIAASTAHAGSVGAQAVRAVERLGVRAIMTTGASDAPPVRETGRVSV
                     VAEAPHGALLAHADALVCNGGGGIVARGLAAGVPMVVLPNHGDQRENGYRVARSGAGV
                     VARTPREIPRALARIVRRPSYRAAAQSIAAQAARSDGPATAAIAVESLQKQREAG"
     gene            complement(4438..4824)
                     /locus_tag="VM841_06425"
     CDS             complement(4438..4824)
                     /locus_tag="VM841_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HVL89856.1"
                     /translation="MAIKFLSDEWLSEVENRLNSHEGFQAAAKGQAAKLQQEVTGTPE
                     GDISYGFILEDGKVQLVRGEVEGPEATITQDYDTAAALSKGELNGQAAFMSGKLKIGG
                     NLMKMMTLQGVLTQMPAALGDMDVEY"
     gene            complement(5566..7020)
                     /locus_tag="VM841_06430"
     CDS             complement(5566..7020)
                     /locus_tag="VM841_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89857.1"
                     /translation="MKRSLRRFTLFAVLVAAIPSFASAALDVACGANATSVVAGRATN
                     VAGDPVQGALIQLRQANKDTLHAQTTTAANGTYKICTTHDTYDVRADTGSGGLYATAN
                     QTITTFTNPAKTTDFVLEYKLNQTITPQAVSIPVAGSSTSVTWRVQSKAPAGTQMRLT
                     LDHLGATVLMSAAAPAGGWNIWTYTAVLAHPLPEGFRWAKVAGYDASNRRITELGQDP
                     YLVDNQAPRFGPATTGSVDCGGSHLAGPFTPFATTNPWALITVGVCDPESNFGRSSLD
                     PYTVSAQIFDPNGNPVTTGSLILNQLSIQYLPSGPMSLGTYQFRFSIRDHAGNLATSP
                     QYALLVEKSGGSVPTLSGFVPGNLGEGSNLGIVIGSGFTTPASRAVVAFYAKDADGQG
                     DLSFGSLRVRVYGPDGNTLLYDYDSSLPPVCSPVQAPPGNTGCFNQGTGRFEASGFSL
                     FGRPPGLYSATASISDHSGNSVTRTWRWFQVLTL"
     gene            7294..8988
                     /locus_tag="VM841_06435"
     CDS             7294..8988
                     /locus_tag="VM841_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89858.1"
                     /translation="MLRARRRLRTGEESGFSMMEVAVTIGIIAMLVVSMTAALGNGFK
                     YLLISKQRAAATQAANAVLERARAVSQNDWTALGLVSSDLAGDPAIAIGSCGGASAAM
                     FGGEPVVTAGTTTSNPLYPHVATQSVGSTAVTIKTYVTGVTSSGSCSVSDHAYKRVTV
                     VATWAKAQPGTDSTIRLVTYLSPVSRASNPDFSGTASYSGGSLIGNFTAIGSALQSTA
                     IYPAQSAADGRSVGQIVTYKGTGISALGMVSGASTAQHTASSAADDDSLTAGIPKHAC
                     DPTSCQPPSPPNCSTFSASNNWSDFLGGMVGSLMQGSAGSCSSVADASDGLPITRNRA
                     ALNTTLAMRGMVPAGGVLPSFLVDLMEVGSGWTTTSSVDRSLSSNQARLASTASTTLS
                     AVPFLRFNVSGVLNAHRGVVEFSGGTFTANAHAGDGAAAPSASGTMTFAVLDPNAVIT
                     GCTGRDGIYCTIVVNPHAAGFAGYDRSFDVVYDFPSVLPAARFVMSTSIKVFVPSKTQ
                     SSSGGTTTQATMSFALPKITSTLTVQAPVGSTLATATDTIDFGQLVTAAFYSPPSP"
     gene            8985..9566
                     /locus_tag="VM841_06440"
     CDS             8985..9566
                     /locus_tag="VM841_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89859.1"
                     /translation="MRILRIVRTPRDERGLSVLELLVGVTLLSVMSAAAMAMLVSTQN
                     RVRSTSKTTESLDGARTTLTRVEREIRDGAKIYEDSDCPVTTCLTLAVIPPGGSTVED
                     VRYRYDLASKTVFRTVGARHPATNTFVPAGQEVPMVSHVQNGTTTPVFCRKAPCTTPS
                     ETGAVQVTLVVNADEAAPSQGVTLTSYVTPRNQ"
     gene            9566..10927
                     /locus_tag="VM841_06445"
     CDS             9566..10927
                     /locus_tag="VM841_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVL89860.1"
                     /translation="MAAGFVRSRRGQQGASLITAMFVSFVMMGLVALMFSQVIHTNES
                     TGRDRQKMTVFHVAEAGVDQAISQLASNPAYAGTGGTAVPVTANGATIGSFTTTVIDP
                     GPGITSDRVIRSTGVLSSGTQQRVVKATVRLAPLGGFDFAIFSAATLAVSNNLTVVGS
                     TYSAQAATLQNNTTVTGDVTSPANITTANNSTINGSVWSGGSIVISTGTTITGNVLAT
                     GSVTIQSNAKVTGDVRAASITNNGTIQGQSVLASQPSPATRTLPAFTYDPANYPAPLG
                     PPVNTTSTGFQLCWQSGAGCPLGVSRTAMRGVYYISDGGTVTGPSQRTTLAGDLYIIT
                     NGRVVIDRDFNTATSDTYRLVVISTSTSVSPAAVTWSNNVSLPPNIQTFIYTPGKVTF
                     DNLKSFSGIVYGGQVQTDQNFTVAYEPALRDVVKGFSWDLSSSGLYEIKPLSWEECGT
                     AAC"
     gene            complement(10932..11975)
                     /locus_tag="VM841_06450"
     CDS             complement(10932..11975)
                     /locus_tag="VM841_06450"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVL89861.1"
                     /translation="MNPIHVAVVGATGAVGSEVLRILEERRFPLASLRPTATARSAGK
                     TLRFAGEDLAVVETSEQAFDGIDVAIFDTPDEAALAWVPVAQKAGVVCVDNSAAFRLR
                     DDVPLVVPEVNPEAAATHEGIIANPNCTMVTLLMPLAPLHRAAHARRVIASSYQSVSG
                     AGVAGVEDLYEQLEKLTPERDAVRDGAVDGLVPAGRAFAHPIAFNVIPHVGGFDADGA
                     TSEERRVRDETRKILAAPDIDIFATTVRVPAIRAHGVAAWVEFESEIDPATARQILSA
                     APGVELADDPASALYPTSLMASGKDKAYVGRIRSGGAPNTLGFFTTCDNLRKGAALNT
                     VQIAELLLERGLL"
     gene            complement(12028..13272)
                     /locus_tag="VM841_06455"
     CDS             complement(12028..13272)
                     /locus_tag="VM841_06455"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HVL89862.1"
                     /translation="MSLIVQKFGGTSVGSAERIKIVAERVCATRREGHDVVVVVSAMG
                     HTTDELVSLANQVARKPPPREMDMLLTAGERISMALLAMAIEDQGLSAASFTGSQAGI
                     ITDTEHGRAKIVDVRADRIKEAVAAGKIAIVAGFQGVSTALDVTTLGRGGSDTTAVAL
                     AAALKADACEIYTDVDGVYTADPRIVPNARLLHVVSYEEMLEMAATGARVLMLRSVEF
                     ARNHNVPLRVRSSFSYAPGTWIKEEDERMEKAIISGVTHDTSEAKVTIQHVPDVPGIA
                     AKVFRTLAQEGLNVDMIVQNVSMAGLTDISFTISREDGERAVDTIKSLMPEVKAEGVS
                     YDDGIAKLSLIGAGMRTHPGVAADMFSALADAGVNIEMISTSSIRISCVIREEDVTKA
                     VKVVHDTFELTDESVIREEHPR"
     gene            complement(13318..13959)
                     /locus_tag="VM841_06460"
     CDS             complement(13318..13959)
                     /locus_tag="VM841_06460"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HVL89863.1"
                     /translation="MSRLAWIAIAYAAGTLPSPYLVALAGRRRDVIAQMRRDESPGDA
                     HFLVVSKMSRALGILAIVLDMVKGFVPALVARATDQDAVTLAWIGLAAVAGHSYPPYF
                     RRAGGRGLTTAAGVTLVLTPKAMVATGIISLAGTVARKGGFGTSIGFALLPAFVAIFG
                     YTGALVAMAGGITALIAIRRMEGARDDRRAGVPVGRILLGRLLFDLPRGKHDL"
     gene            14088..15404
                     /locus_tag="VM841_06465"
     CDS             14088..15404
                     /locus_tag="VM841_06465"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL89864.1"
                     /translation="MPTAAGETQPGLSFDGFKAVMSNRSFRMLFWGQAVSALGDWVGT
                     LAFIAAADQLSGGNAFAITGVLVLRLVPTLFATPIGGVVADRFDRKRIMIWSDIARFA
                     VILATPFVPNLIALYIFAFVHECFSLVFLPARDATIPNLVPADRLEPANALVMGSSFA
                     GIPLSGPVYGAMAAIALHFPAVPTQSRWLEHPWGLAFVFDAFTFLLSAWFIARVTMPP
                     REQRDGQASERFGAMLRGGIGYVRSKPILIGLSFAVSLGLLGGGVLFAKGISYVKETL
                     GGNDVAFGYLMGIFGGGMVLGFVGSQFHSERGAMWTLRGALVAMGGVLITMALIPALW
                     MAYIMASVFGLTFALAIIIGMTQAQLLTTDEMRGRVMAIVHILVRLALLVGAVMAAGI
                     GAIFKDGLRVPGLNYRADSNQVALAIAGALIAAGVVGVRGKRERSG"
     gene            complement(15454..16047)
                     /gene="recR"
                     /locus_tag="VM841_06470"
     CDS             complement(15454..16047)
                     /gene="recR"
                     /locus_tag="VM841_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HVL89865.1"
                     /translation="MFEGPVQDLIEAMRRLPGIGAKSAQRLAFHLLRAPEEEVRALAE
                     AILAAKERIAICGTCFNVSEGPQCAYCRDQRRDGTILCVVEEPADIIAVERTQEFRGR
                     YHVLGGHISPMDGIGPEDLRIKELLGRISGGEVKEIILATNPTVEGEATAIYLAGLVK
                     PLGVRVTRIASGLPVGGDLEYADEVTLGRALEGRREV"
     gene            complement(16049..17743)
                     /gene="dnaX"
                     /locus_tag="VM841_06475"
     CDS             complement(16049..17743)
                     /gene="dnaX"
                     /locus_tag="VM841_06475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HVL89866.1"
                     /translation="MSQVALYRRWRPQTFADVVGQEHVAHTIQGQVREDRIAHAYLFS
                     GPRGTGKTSTARILAKALNCEQGPTPEPCNKCDACRSITDGTSLDVIEIDAASHGGVD
                     DVRELRDNVLLAPALARKKIYIVDEAHMVSTQGWNAFLKTIEEPPSHVVFVFATTEPE
                     KVIGTIKSRCQRFDFRRVSGDDIHRHLARICAEERIEAEDAALAVIARHADGGMRDAL
                     STLDQLASSGTVTAERAVDLLGSAPVETLFEFGESLAARDAGTALRMLARLVDEGDDL
                     RTFTRQALEHFRGLLFLQRVPDGGGLIDATDESRARLSAQADRFQPAHLVHVLKLLAD
                     AQAEMRQQAPPRLTLELAVVRAALPDADASPEALLSRIERLERMLESRPAAAASPEEA
                     APDAAPAPAQQPPAAPQRRPIPPKRSARARAQEPPKAEDDLPAPDPEIAASLDFDRVS
                     RDWPVLLEETRKRSKQLYALLAAMRPIGYANGSLDLEAKFDYHASQIAELKHARAFAD
                     SFNAVFGTPVRIRATVASDAPPPAPDPPTAESGPPVDPVEALKDALGAEVIDEIEG"
     gene            complement(17803..18243)
                     /locus_tag="VM841_06480"
     CDS             complement(17803..18243)
                     /locus_tag="VM841_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HVL89867.1"
                     /translation="MIAVDTNILVYAHRRDSEFHRTASRAVRMLAESGGPWAIPWPCV
                     HEFLSIVTNPRIWRKPTTLKQALDQVEAWIESPGLTLLAETGDHWPALRETLSRTRTS
                     GPKVHDARVAAIATTHGVRELWTADRDFSRFPNLTVVNPAASAE"
     gene            complement(18240..18485)
                     /locus_tag="VM841_06485"
     CDS             complement(18240..18485)
                     /locus_tag="VM841_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HVL89868.1"
                     /translation="MKTTIEIADAVLAEAKKLASAERTTLRELVEQGLRHVIAQRSRK
                     QSFRLRDASVDGTGLQPEFADQDWARIRQAAYAGRGG"
     gene            complement(18814..18912)
                     /gene="ffs"
                     /locus_tag="VM841_06490"
     ncRNA           complement(18814..18912)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VM841_06490"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(18961..19050)
                     /locus_tag="VM841_06495"
     tRNA            complement(18961..19050)
                     /locus_tag="VM841_06495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19014..19016),aa:Ser,seq:gga)
     gene            complement(19146..19237)
                     /locus_tag="VM841_06500"
     tRNA            complement(19146..19237)
                     /locus_tag="VM841_06500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19201..19203),aa:Ser,seq:cga)
     gene            complement(19289..19735)
                     /gene="tadA"
                     /locus_tag="VM841_06505"
     CDS             complement(19289..19735)
                     /gene="tadA"
                     /locus_tag="VM841_06505"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HVL89869.1"
                     /translation="MSDDDFMRMALAEAEAALAHDDVPVGCVIVRDDVVIGRGHNARE
                     RDQDPTAHAEIIALREAAAASGSWRIEDATLYVTLEPCFMCAGAMVLGRIPRLVFGAI
                     DPKAGACGSLHDVVRDPRLNHRMDVTGGVLAAECGQLLRRFFAARR"
     gene            complement(19732..20139)
                     /locus_tag="VM841_06510"
     CDS             complement(19732..20139)
                     /locus_tag="VM841_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HVL89870.1"
                     /translation="MTTPGPPYEPSGGQMIVRGLAKRCPRCGGGNLFDGWFTMRERCP
                     RCHLKLAREQAHFVGAMAINLVVTEIAFGILLVTWAALAWPDPPWLAITLTAVGMNLV
                     LPILLYPFSKSIWTALDLWMHRFDPIDRTEIEL"
     gene            20219..20794
                     /locus_tag="VM841_06515"
     CDS             20219..20794
                     /locus_tag="VM841_06515"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVL89871.1"
                     /translation="MSAPILIFLHGPMAGKRIPIDRDELTIGRSDTNDVVLDDKLVSR
                     LHAIVMRRGDVVLVEDLGSNNGTYVQDERLHAIRQLHDGQTITIGTSRVMFEDASASG
                     EETTQIAAGGGMAAPATFTQRQMQVLRLLARGLSNKQIGERLFVSERTVKAYLSSIFD
                     KLEVGNRSAAITAALRLGLIDIPGDADASAE"
     gene            21109..21372
                     /locus_tag="VM841_06520"
     CDS             21109..21372
                     /locus_tag="VM841_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89872.1"
                     /translation="MKKMLALLMLVFAIAAAPMVSSADECGESEIARVCVTDDGVIVV
                     DGAEGNEDPLDGYVTIDVQNGEMCADSDGNYDDPDADPNCYSL"
     gene            21452..22345
                     /locus_tag="VM841_06525"
     CDS             21452..22345
                     /locus_tag="VM841_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HVL89873.1"
                     /translation="MQRLVAMPGEYSLIATLSPLRHGGYDPTVTIERNAVTRAYRIGG
                     EPVTVRYERVADGVRVTAWGPAGEKALDEAPSVLGSFDDPSAYRPVHPILRDLHRGRP
                     GMRMTATRAVFEALVPSVLEQKVTGREATNAWARFVRRSGEAAPGPFDLMLAPVPEAI
                     AAMPYYVWHPFGVERLRAETIRRACARAGRVRELAAMDAAGARRRLRALPGIGVWTAA
                     EVTRVTHGDADCVSIGDFHTKNLVSWALAGEARGTDERMMELLEPEAGHRGRAVRLLE
                     MAGMAAPKFGPRQRIRRIAHI"
     gene            22420..22806
                     /locus_tag="VM841_06530"
     CDS             22420..22806
                     /locus_tag="VM841_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL89874.1"
                     /translation="MAHKNVEILRRTDETMQAGDVEGFFGTFTDDVVIHVPGRHSFAG
                     SYKGIDQFRGLFEKFMQHAGDYSFENHTYLADDEHGVAMQHSTYEKQGRRLAMDEVFI
                     CHFRDGKVSEMWYLAGDQQALDEFLG"
     gene            22882..23799
                     /locus_tag="VM841_06535"
     CDS             22882..23799
                     /locus_tag="VM841_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89875.1"
                     /translation="MPRRTRFADVLPAIRRAFGAAPDHAGRLDEGAYSPAGRWLVRFA
                     DGRSAFVKAEWEASDGHGTAVEYLVYSNVSAPPVPRLIAYEAGSTEAPRVIVTEDLSH
                     ARWGAPVTRKDATLFDAAVAALEGVAAPEGIRDIPFRPQWDALVRDPAPLIAAGVCDA
                     RWIERYGERLSAAEATVDPCGSGLVHCDLWLQNWCRADRGVVMVDWAGAARGSLDVAR
                     AWGEAAVRAAGGPSNVIVPKGNAGWAAFMAGLAAQFAAEDHGEPASPRLVETIRREAA
                     ATLAWACEELEIEPVTHAPGFDPGGPWRP"
     gene            complement(23807..24478)
                     /locus_tag="VM841_06540"
     CDS             complement(23807..24478)
                     /locus_tag="VM841_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL89876.1"
                     /translation="MRATKWWVAILTVFVGAITGVAAFGANIGFSGGGGGRVSSQSNE
                     ISPDVVAAVEQARWEAVQRMEAARIEAERLAEKQRREAEKQRREAAIRAKVSRYGLSW
                     PHVDMITSHFGNRRRGFHNGMDILCSKTGGEPIFAAAPGRVIASNNMPVYGRAVILQH
                     QNGTKTLYAHMWSLRVRAGEWVERGQVVGTCGSTGNSTNPHLHFEFYRNGRTVNPLHH
                     LPAWT"
     gene            24832..25458
                     /gene="msrA"
                     /locus_tag="VM841_06545"
     CDS             24832..25458
                     /gene="msrA"
                     /locus_tag="VM841_06545"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HVL89877.1"
                     /translation="MLHRKTTMIEPERALPGRATALSVPERHHALGTPLQGPWPDGSE
                     LAMFAMGCFWGAERIFWRIDGVHSTFVGYAGGYTPNPTYEEVCSGLTGHAEVAVVVFD
                     PARVSFETLLKAFWENHDPTQGMRQGNDVGTQYRSAIYTYGDEQHDAAKRSRDRYQAL
                     LRDKGFGDITTEIAPAPEFFYAEDYHQQYLSKNPNGYCNHGFCQVEYS"
     gene            25511..26626
                     /locus_tag="VM841_06550"
     CDS             25511..26626
                     /locus_tag="VM841_06550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVL89878.1"
                     /translation="MAGRDSVIDLVRSGATTTVVVYHWVFTIIVWRPDGPHADNPIGY
                     VSGLWFLTWILQVLPLFFMAGGFTHSLAWAKYRETPKAWWRFVARRAGQLLGPAVLLV
                     GVVVGAALTVASLRAGSDSWFARGVILVLSPLWFLVVYLVLVATVPLWDRLHRRFGEL
                     VPLGLAALAMSVDVLRFRYDVGWIAFANLVFVWAACHQVGWSWDRLSAAPKRFGHALI
                     LIGFAALAGLTNMGLYPRSMVGTTSPLDRFSNMGPPTLPIIALLTLQLGLVVANRDRL
                     AAWAERPRIARRVAWMSRNAMPLFLWHSVGFAAFFIAMRSWTTVPESPTLVWWLTRPL
                     WLIGPALATLPLVAASTRLLRPSGAVTGARKRTAVPA"
     gene            26631..27317
                     /locus_tag="VM841_06555"
     CDS             26631..27317
                     /locus_tag="VM841_06555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVL89879.1"
                     /translation="MGAAWLAAALVAYNAAANRWKPFHSWAYLPANLTLAAAVVWLGA
                     AAFDLSAGEMGFRGGSIRVGAAIGVALGVAAMVPVYAALGFERGRALLRDRRLSGVRG
                     AKAAFVLLVRIPIGTALVEETVFRGVLLGSAMSHGTAQAVALSSVAFGLWHIVPTWIL
                     ARKNRLNVAVVPAGVVVTGAAGAFLAWLRIETGGIAAPVALHVLVNSPAAAAAMIALR
                     LPAASRGPSR"
     gene            complement(27295..>27885)
                     /locus_tag="VM841_06560"
     CDS             complement(27295..>27885)
                     /locus_tag="VM841_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89880.1"
                     /translation="ASGDQIIVTMKHGSVPSTTGWVFAARPAQNPAGATLAKCTFTVV
                     SSYAANIVDAAKNPLSARAAPATVLHAPGMLAAAPVTGDRNNDGVIDEIQLVSHHAFD
                     ASSVSSGALTRLTITDPSNASVRATGLTVRSVAGQVLTVGFTPAAGWSAATTPMVAYT
                     TTADCMGSVLRILTPVGDQQCLSGFSLRAINGTGRG"
BASE COUNT         4205 a         9397 c         9737 g         4546 t
ORIGIN      
        1 gacggccgat gccggtgaat ccccgtctgg gctgccggcc ggcgcgccgg ccggcagccc
       61 agacggggat tcaccggcat cggccgtcgc gccgccgtgt ccggcaggcg ccgggtcttc
      121 gatccgaagc ggggggattg ctccggtcgt acccgacgcc gccgcacgtg gcggcgcgtt
      181 cgccgagggg cgctcgccag gacgctcgtc cggctgcgac gacgcgcgcg gctccgcgga
      241 aggttccggg ccgggctcgg gttccgagga acggcacgag cccagcagcg ggccggcgag
      301 cgcccggatg tcgcagacgg ccgatcgcac cggtccgtcc ttcgttccga aggcggtcgc
      361 gggcgacgcc accagcatga tcagcatcgt cacggcgata tacgtccgcg cagggtgtgc
      421 ggcatgcttg gtcgtgtttc ccccgctggt cggcatggga ccatcctggt gggaagcgtc
      481 cgatgcgtcc atggcctcga tgggctaaat cccgtagcgg aatgggacta gtcccgccgg
      541 agacgctggt gggaaaaggt gcgaagcgcg cgtcaggcca cgatcgtgcc gaccagcacg
      601 gccgaggact tgccggactg atcgaccgcg atgatcttga cctgatatgt gccgggaagc
      661 accgatccgg cgggagtcca cgaataggag aacccgccga gcggtacgcc cgtgcgagcc
      721 tcttggaaca cgacccggtt ctgcgcgtcg aagacctgca cgcccaccga cagcgacgac
      781 gacgcgtcgt cgccgaggct cgcggtcacc acgacggcct ggcccgtcgc gcgcacgccg
      841 tggctcgcag ccgcgctcgc gatcaccggc gcctggccgt cgatcatcag cacgccgacc
      901 gtcttgggac cccagcgtga gccgtcgaag gcttgcacgt agatcggatg cgggcccgcg
      961 gccaggccgg tgggaacggc gaccgtcgcg cgcagcgtct cggcggcctg gccgaaggca
     1021 ccgtcggcgg cgctgagcgc gatcgcgtcg gaggagggcg agccgatgaa gacgcgcgcc
     1081 gccttgacgc cggccggggc cgccgcgctc gcggtgatgg tggtcgtgcc cgcgggaccg
     1141 ggaacggtgc ttcgcgcgtt cgtcgcggcc ggaccgtcgc gcagcgcacg ctcggcgttg
     1201 atgcggccgt ggccgaagcg atcgtcgcgg ccgggcgggc cgaggtcgtc ggcgctgcgc
     1261 tcgatgatcg cgcgcacttg cgccggggtt cgtgacgggt cctgcgcgaa cagcaacgcg
     1321 gcgagaccgg agacctgggg cgccgcagcg ctcgtgccgc acggcggcgc gacggtcccg
     1381 cctcccacgt tcagcgagac gacgcgttga gccggcgcgg cgacactgac gttggcgttg
     1441 tggttcgagc acgacgcgta cgaatcggcg tcgttggttc cggcgaccga catcacgtcg
     1501 ggatacgagc ccggatagaa gggcgcgctg ccggtgttgt tgccggcggc cgccacgatc
     1561 agcgcggggg atgtaccgcc ggccgcggtg cggatcgcgt cgcgaacggc cgcggagtcg
     1621 gcggtcaagc cgagagagag gttgatgacg cgcgcgcctc gctgccacgc gtagacgatc
     1681 gcgtcggcga tggaggcggc gtcggccgtg cccgctccgt ccacgaccgt gagtggaagg
     1741 attgcggcgg tatggcccag cgcggcgatg ccggtgccgt tgtccgtcgc ggcggcgacc
     1801 agcccggcca cataggtgcc gtggtagtgc gcggcgccgt tctgctgtga ggcggggatc
     1861 cagtcgcgtg catccgccat gaccagctgt ccgccgtcgc gcgcgtcggt cgatgtcgag
     1921 ccgcggttgg caaagtcggg atgcgccgcg tcgatcttgg tgtcgagcac ggcgacggcc
     1981 acccgctcca tggagcgcag ctgctgcgcc gagggaaacg actcccatga cgggttggtc
     2041 atgcgcagcg cccactgccc tgcggtggcg cacccgccgg tcacgcacgg gtcgtccggc
     2101 tcggcagcca gacgcgcgag atggttcggc tcggcgacgg cgaccgtagg atcgtttcgg
     2161 tacgaggcga gcaccgcatc gaagggcagc gacggatcga acgccacccg ctggtagccg
     2221 atcacaggaa tcgaagagat cacgcgagca ccggcgcggg cgtgcgccgc ggcgattaac
     2281 gccgagggtg ttcccggcgc gaagcgcacg agcgcctcac cggcgcggaa tgccgtcggc
     2341 gacgaggccg ccggagcgac gcgcggcgcg acaaccggca ccacgcatgc gatggcgagc
     2401 agggcacgaa gaacgcgtgt tgacgacaat ggatcctccc tcgatgtcgc gggtgaaaac
     2461 gacgagcggc atccgggtgt tcgggtggtt ttccgacatg gcccgcaatg actagtccgc
     2521 ccccgtcggt acgcaaagat ggcccttcgt cggaaatatc ggcccttctc cgcgatagag
     2581 tgcggcgata tgaaccgacc gcgcttgtcg ctcgccgcct atccggccgc cttcgttgtc
     2641 ggcgcgattc tcgggacgtt ctgggaccgg cttcacgtcg tcgccggcac gctcgtctac
     2701 gccgacgaag gcgtgttcgg acagcccttt tggatcccgc tggaattcgg cgcggccgcg
     2761 gtcgcgggcg tgatggtctt caccctgctc ggcgacccgg caccgcggcg cgagtccccg
     2821 cgtgtcgcgg cgatcgagac gctctggatc tccgcggtgt acgcggcgac cgcgttcttc
     2881 gaccgatggc cgtgggcgct cgcggcgctc ctgctgatcc tgctggcggt acgcgcgaag
     2941 gacttggcac cgatcgtggc gggcagcccg attccgtcgc tcgcgctgct gctcggcggg
     3001 ccgctgttcg aggcggcgct gatctcgagc ggcgtgtact cgtaccgcaa cagccagcta
     3061 gggccgctgc cggtctggct gccggtgctg tgggcgcacg gcgtgctgtt catgatgcgc
     3121 ttcagcgagg cgatgctgct tcgcttcggc gtacgccggc gcatccagcc ggcgaccgcg
     3181 accctcacgc cgaccgatcc ggagcctgat ccggagccgg acccgatcga ggactagccg
     3241 tgcgcgtgct gtgcgccgct gtgaccggcc ccggtcacgt gtatccggta atggcggccg
     3301 cgcgagcgct cgcggcgcgt ggccacgagc ccgtgctcgc cttcgaatcc cgattcgagc
     3361 atctcgccga gcgagagggc atggccttcg tgccgctgcc cggcgtgcgt tccgcggacc
     3421 cggacgcgtt caagccgtac gagatctcgc ttgcgatcgc acgcgcgttc gctcccgagg
     3481 ttcgaaggat ccggcccgac gccgcgctcg tggacatcat cacgcacggc gtcgcgctcg
     3541 catgcgaggc cgcaggcgta ccgttcgcga cgctgtgccc gcatccgctg ccgtacccgt
     3601 cgaaggatct cgcgccgttc ggagcggcgc ggccgccgcg tcgcggcgcg tggggccggc
     3661 gccgcgacga ccgcgcccgt gcccggcatc tggccgatct cgagcgcggc cgcgacgagt
     3721 gcaacgcgat ccgcggggcg atgggcctgc cggcgctcga ccggctcgac gtcgcgtgga
     3781 gccgggaggc gatcctggtt gcgaccccgg ccgcgctcga gccgccccgc agcgactggc
     3841 ccgcgcacgc gcgcgtcgtc ggtccgtgcc tgtatgaacc cgacgggacc gcgcccgcga
     3901 tccccccggg tgacggtccg ctcgtgctga tcgccgcgag caccgcgcac gccggaagcg
     3961 tcggtgccca ggcggtccgc gcggtcgagc ggctcggcgt gcgagcgatc atgacgaccg
     4021 gcgcctccga cgcacctccg gtacgcgaga ccggccgtgt atcggtggtg gccgaagcgc
     4081 cgcacggcgc gttgctggcc catgccgacg cgctggtgtg caacggcggc ggcggcatcg
     4141 tcgcgcgcgg gctcgctgcg ggtgtgccga tggtcgtgct tcccaatcac ggcgatcaac
     4201 gcgagaacgg ctaccgcgtc gcgcggtccg gcgccggcgt ggttgcgcga acgccacgcg
     4261 agattccgcg cgcgctcgcg cgcatcgtgc gccgcccgtc gtaccgagcc gccgcgcagt
     4321 cgatcgcggc gcaggcggcg cggtcggacg gacccgcgac ggcggcgatc gcggtcgagt
     4381 cgctgcaaaa acaaagggag gcgggctgat gcccgccccc ctcccgaacg gaggtcttta
     4441 gtactcgacg tccatgtcgc ccagcgcggc gggcatctgc gtcaagacac cctgaagggt
     4501 catcatcttc atcaggttgc cgccgatctt gagcttgccg gacatgaacg ctgcttggcc
     4561 gttgagctcg cccttggaga gggccgcggc ggtgtcgtag tcctgggtga tcgtggcctc
     4621 ggggccttcg acctcgccgc ggaccagctg cacctttccg tcctcgagga tgaagccgta
     4681 gctgatgtcg ccctcgggtg tgccggtgac ctcctgctga agcttggccg cctggccttt
     4741 ggccgcggcc tggaagccct cgtgcgagtt gagccggttc tccacctcgg agagccactc
     4801 gtcggaaagg aacttgatcg ccatcgaacc ccctcgaatt tcgtgccggc tcctcgcccc
     4861 ctccggcgcg gctcaccgag ccggaccggg cggtggcgca aggctatcgg ggcccggccg
     4921 cgttggcaaa accgggcttt ttccgggctt tctcgtcaag gccgctgccc ggcggggcgg
     4981 ccggtgcttg cctgcctgaa ggaaaatcgg acatcggccg ataccgaatt cgagggtcgg
     5041 aacgagaggg ttccgctttc ctcgggggag cggaggcggg gccggagagg atggcagcag
     5101 gtggaggcgc tgtgggaagc cttgcaggcg aacccgcggc tgttcggcgc cctgggagga
     5161 gcgctcggac tcggactcct gacatgggcg gtgatccgtt ccagctaccg gtatcggtag
     5221 cggcggaccc gaatggctcg gcgatggagc cgtcccggcg aatcggcggg acggctccgt
     5281 cgagttctac agcgcgagcc acacctcggt atccggcacg aacgccgccc acgcgaacca
     5341 gaaggtaggg ggacggccgg gcaggcgagc ggcgacggac cccgaaaacg cggcagccgg
     5401 agcgcctgcg aggaacactc cggccgagcc gcgaaacaaa gttataggag cccgcaggcc
     5461 gccttggcaa gcgatccgga gcgttgccga tggtattcgt cggacatgcc gcctaatcct
     5521 tctggagccc gcggccgtga atggggcaaa agccgcgtgt cggcctcaga gggtgagaac
     5581 ctggaaccaa cgccacgtgc gcgtgacgga gtttccggag tggtcggaga tcgaggccgt
     5641 cgccgagtac aagcccggtg gccggccgaa cagcgagaag cccgacgctt cgaaacggcc
     5701 ggttccctgg ttgaagcagc cggtgttgcc gggtggggcc tggaccggcg agcagaccgg
     5761 cggcagggac gagtcgtagt cgtagagcag cgtgttgccg tcgggtccgt agacgcgcac
     5821 gcgcaggctg ccgaacgaca ggtcgccttg gccgtcggcg tccttggcgt agaaggccac
     5881 gacggcgcgc gaggccggcg tcgtgaagcc cgatccgatc acgatgccga ggttcgagcc
     5941 ctcgcccaag ttgcccggca cgaagcccga gagcgtcggc acgcttccgc cggatttctc
     6001 gacaagcaac gcgtattgcg ggctcgtcgc gaggttgccc gcatggtcgc ggatcgagaa
     6061 gcggaactgg taggtgccca gcgacatcgg ccccgacggc agatattgaa tcgagagctg
     6121 gttcaagatc agcgatcccg tcgtgaccgg attgccgttc ggatcgaaga tctgcgcgct
     6181 caccgtgtac gggtcgagac tggaccgtcc gaagttcgat tccggatcgc acacgccgac
     6241 ggtgatcagc gcccacgggt tcgtcgttgc gaacggcgtg aacggtcccg cgagatggct
     6301 gccgccgcag tcgacgctgc cggtcgtggc cggcccgaag cgaggagcct ggttgtccac
     6361 gaggtaggga tcctggccga gctcggtgat gcggcggttc gaggcgtcat agccggcgac
     6421 cttcgcccag cggaagcctt cgggcagcgg gtgcgcgagc acggccgtgt aggtccagat
     6481 gttccaccca cccgcgggcg cggccgcaga catcaagacc gtcgcgccaa gatggtccag
     6541 ggtcaggcgc atctgggttc cggccggcgc cttcgactgc acgcgccacg tgaccgacgt
     6601 gctcgatccc gcgaccggga tcgagacggc ctggggcgtg atcgtctgat tgagcttgta
     6661 ctcgaggacg aagtcggtgg tcttggcagg gttggtgaac gtggtgatgg tttggttggc
     6721 cgtcgcgtac agaccgccgg agccggtgtc agcccggacg tcgtaggtgt cgtgcgtggt
     6781 gcaaatcttg taggtcccgt tcgcggccgt ggtggtctgc gcgtgcaggg tgtccttgtt
     6841 cgcctgccga agctgaatca gcgcgccctg gacgggatcg ccggcgacat tggtggcgcg
     6901 gcccgccacc accgaggtcg cgttggcgcc gcaggcaacg tcgagcgcgg ccgacgcgaa
     6961 tgaagggatc gcggcaacca ggacggcaaa caaggtgaac cggcgaaggc tccgcttcat
     7021 gttcgagcgt ttcgccgcat cgctccgcaa acccttcgat tccccgtaga gcccggtttc
     7081 gttcggttca ggttcgttcg aaagttcctg tttgcacccc ggcgtcgctt cggatgcgcc
     7141 gtctctagcc cgtacgtgcc gctcgttttt agccgcgacg ggctatctcg aatagggcgc
     7201 ttcaacccga agaagaagcc agaggccgcc tgcgtggcgg agggctcgga gaccggcctc
     7261 gatcggagga tcctcattcg cgcttgtgcg tcgttgctgc gggctcgccg gcgtctgcga
     7321 accggcgagg agtccggctt ttcgatgatg gaggtcgcgg tcacgatcgg catcatcgcg
     7381 atgctggtcg tttcgatgac cgccgcgctc ggcaacgggt tcaagtacct gctgatctcc
     7441 aagcagcgcg cggcggcaac ccaggcggcc aacgcggtgc tcgaacgcgc acgcgcggtg
     7501 tcgcagaacg actggaccgc gctcggactg gtctcgagcg atctggcggg cgatccggca
     7561 atcgccatcg gctcgtgcgg tggagcgtcg gcggccatgt tcggcggtga gccggtcgtc
     7621 acggccggca ccaccacgtc caacccgctg tatccgcacg tggcgaccca gtcggtcggc
     7681 tcgaccgccg tcacgatcaa gacctacgtc accggcgtca cctcgtcggg cagttgctcg
     7741 gtgtccgatc acgcctacaa gcgagtgacc gtggtggcga catgggccaa ggcgcagccg
     7801 ggcaccgaca gcacgatccg gctggtgacc tacctgtcac ccgtgtcgcg agcctccaac
     7861 ccggacttct ccgggacggc ctcgtactcg ggcggcagcc tcatcggcaa tttcacggcg
     7921 atcggctcgg cgctgcaaag cacggcgatc tacccggcgc agagcgccgc ggacggtcgc
     7981 tccgtcgggc agatcgtcac ctacaagggc acggggatct cggcgctggg catggtgagc
     8041 ggcgcgtcca ccgcgcagca cacggcctcg tcggcggccg acgacgactc gctgacggcc
     8101 gggatcccga agcatgcctg cgacccgacg tcgtgccagc ctccgtcacc gccgaactgc
     8161 tcgaccttca gcgcgagcaa caactggtcg gacttcctcg gcggcatggt cggctcgctg
     8221 atgcaaggat cggccggttc gtgctcgtcg gtcgccgacg cctcggacgg gctgccgatc
     8281 acgcgcaacc gggccgcgct caacaccacg ctcgcgatgc gtggaatggt gcccgccggc
     8341 ggggtgttgc cgtcattcct cgtggacctg atggaggtcg gctcgggctg gaccaccacg
     8401 tcgtcggtgg accgatcgct atcgagcaac caggccaggc tggcctcgac cgcgtccacg
     8461 accctgtcgg ccgttccgtt cctgcgtttc aacgtgtccg gcgtgctgaa cgcccatcgc
     8521 ggggtcgtcg agttctcggg tggaaccttc accgcgaacg cgcacgcggg tgacggggcc
     8581 gctgctccgt cggcgagcgg aaccatgacg ttcgcggtcc tcgaccccaa cgcagtgatc
     8641 accggatgca ccggccgcga cggcatctac tgcaccatcg tcgttaatcc gcatgcggcg
     8701 ggattcgccg gctatgaccg cagcttcgac gtcgtctacg acttcccgag cgtgctgccg
     8761 gcggcccggt tcgtcatgag caccagcatc aaggtcttcg tgccttccaa gacgcagtcc
     8821 tcctcgggtg ggaccacgac acaggccacg atgagcttcg cgctcccgaa gatcacgtcg
     8881 accttgacgg tgcaggcacc ggtcgggtcc acgctcgcca cggcgaccga cacgatcgac
     8941 ttcgggcagc tcgtcaccgc ggccttctac agccctccgt cgccatgagg atcctgcgaa
     9001 tcgtccggac gccgcgcgac gagcggggcc tgtcggtgct cgagctgctc gtcggcgtga
     9061 cgctgctgtc ggtgatgtcg gccgcggcga tggcgatgct ggtctcgacg cagaaccggg
     9121 tgcgctccac gtcgaagacg accgaatcgc tcgacggggc gcggaccacg ctcacccggg
     9181 tcgagcgcga gatccgggac ggcgccaaga tctacgaaga tagcgactgt cccgtcacga
     9241 cctgcctgac gctcgcggtc atcccgcccg ggggcagcac cgtcgaggac gtgcgctatc
     9301 gctacgacct cgcctccaag accgtcttcc ggacggtggg ggcacgccac ccggcaacca
     9361 acaccttcgt gcccgccgga caggaggtcc ccatggtctc gcacgttcag aacggaacga
     9421 ccacgccggt gttctgccgg aaggccccct gcacgacgcc ctccgagacg ggagcggtcc
     9481 aggtcacgct cgtggtgaac gccgacgagg ccgcgccgtc ccagggcgtg acgctcacct
     9541 cctacgtgac gccgagaaac cagtgatggc ggccgggttc gtgcgctccc gtcgcggcca
     9601 gcagggcgcg tcgctgatca ccgcgatgtt cgtgtccttc gtgatgatgg gactcgtcgc
     9661 gctgatgttc tcgcaggtga tccatacgaa cgagagcacc ggccgtgacc gccagaagat
     9721 gacggtcttt cacgtggccg aggccggagt cgatcaggcg atctcgcagc tcgcgtccaa
     9781 cccggcgtac gcgggaacgg gagggaccgc ggttccggtg accgccaacg gcgcgacgat
     9841 cggctcgttc actaccaccg tcatcgatcc cggtcccggg atcacctccg accgcgtgat
     9901 ccgcagcacg ggcgtgctgt cgagcggaac gcagcagcgc gtggtgaagg ccacggtgcg
     9961 gctcgcgccg ctgggcggct tcgacttcgc gatcttctcc gccgcgacgc ttgccgtgag
    10021 caacaacctg accgtggtgg ggagcacgta ctcggcgcag gcggcgacgc ttcagaacaa
    10081 cacgaccgtg accggcgacg tcacctcacc cgccaacatc accaccgcca acaactcgac
    10141 gatcaacggc agcgtgtgga gcgggggcag catcgtcatc tcgaccggca ccacgatcac
    10201 cggcaacgtg ctcgccacgg gaagcgtcac gatccagagc aacgcgaagg tgaccggcga
    10261 cgtgcgcgcg gcctccatca ccaacaacgg caccattcaa ggccagagcg tgctggcgag
    10321 ccagccttcg cccgcgacga ggaccctgcc ggcgttcacc tacgaccccg cgaactatcc
    10381 ggcgccgctt gggccgccgg tcaacacgac ctccaccggc tttcagctgt gctggcagtc
    10441 gggggccggc tgtccgctcg gggtgagccg caccgcgatg cggggcgtgt actacatcag
    10501 cgacggcggc acggttaccg ggccgagcca gcggaccacg ctcgccggcg atctctacat
    10561 catcaccaac gggcgggtcg tgatcgaccg cgacttcaac accgcgacca gcgacaccta
    10621 ccggctcgtc gtcatctcga cctcgacgtc ggtgtcgccg gcagcggtca cgtggtccaa
    10681 caacgtctcg ctgccgccga acatccagac gttcatctac acgccgggca aggtcacctt
    10741 cgacaacctc aagtcgttct cgggcatcgt ctacggcggc caggtccaaa ccgaccagaa
    10801 ctttaccgtc gcctacgagc ccgcgctgcg cgacgtagtc aaggggttct cgtgggatct
    10861 gtccagcagc ggcttgtacg agatcaagcc gctgtcttgg gaggagtgcg gaaccgcagc
    10921 ctgttgaccg gctacagcag gccgcgctcg agcagcaact cggcgatctg gaccgtgttg
    10981 agcgccgcgc ccttgcgaag attgtcgcag gtcgtgaaga agccgagcgt gttcggggca
    11041 cccccggagc ggatgcgtcc cacgtatgcc ttgtccttgc cgctcgccat cagcgacgtg
    11101 ggatagagcg cgctcgccgg atcgtcggcg agctccacgc ccggcgcggc cgacaagatc
    11161 tgacgcgcgg tcgccgggtc gatctcgctt tcgaactcga cccacgcggc gacgccgtgc
    11221 gcacggatcg ccgggacgcg gacggtggtg gcgaagatgt cgatgtccgg ggcggcaagg
    11281 atcttgcgcg tctcgtcgcg gacgcggcgc tcctcgctgg tggcgccgtc ggcgtcgaag
    11341 ccgccgacgt gagggatgac gttgaaggcg atcgggtgtg cgaacgcgcg cccggccggc
    11401 acgagcccgt cgacggcgcc gtcgcggacc gcgtcgcgct caggggtgag cttctcgagc
    11461 tgctcgtaga ggtcctcgac ccccgccacg cccgcgcccg acacggactg gtacgacgag
    11521 gcgatcacgc gccgcgcgtg cgctgcccga tgcagcggcg ccagcggcat caacagcgtg
    11581 accatggtgc agttcgggtt ggcgatgatc ccttcgtgcg tcgcggcggc ctcggggttc
    11641 acctcgggga cgaccagggg cacgtcgtcg cggagccgga acgcggccga attgtccacg
    11701 cacaccacgc cggccttctg cgccaccggc acccacgcca gcgcggcctc gtcgggcgtg
    11761 tcgaagatcg ccacgtcgat gccgtcgaag gcttgctcgc tcgtctcgac gacggccaga
    11821 tcctccccgg cgaatcgcag cgttttgccg gcgctgcgcg cggtggcggt cgggcgaagc
    11881 gaagcgagcg gaaaccggcg ttcttcaagg atgcgcagca cctcggagcc gaccgctccg
    11941 gtggcgccga cgacggcgac gtgaatggga ttcatggtga cgacaggcta ccggagccga
    12001 cgcgccggtt cgactcggat tcgtcgctca gcggggatgc tcttcgcgga tcaccgactc
    12061 gtccgtcagc tcgaaggtgt cgtggacgac cttgaccgct ttcgtgacgt cctcttcgcg
    12121 gatcacgcac gagatccgga tcgaggaggt cgagatcatc tcgatgttga ccccggcgtc
    12181 ggccagcgcc gagaacatgt cggccgccac ccccggatgg gtcctcatgc cggcgccgat
    12241 cagcgacagc ttcgcgatcc cgtcgtcgta ggagacgccc tcggccttga cctcgggcat
    12301 gagcgacttg atcgtgtcga cagcccgctc gccgtcctcg cgcgagatcg tgaacgagat
    12361 gtcggtcagg cccgccatgg acacgttctg cacaatcatg tccacgttga ggccttcctg
    12421 ggcgagggtg cggaacacct tcgccgcgat gcccggcacg tcgggcacgt gctggatcgt
    12481 gaccttggct tcggacgtgt cgtgcgtcac gcccgagatg atggccttct ccatgcgctc
    12541 gtcttcctcc ttgatccatg ttcccggcgc gtacgagaac gacgagcgaa cccgcagcgg
    12601 gacgttgtgg tttcgcgcga actcgaccga gcgcagcatc agcacgcgcg cgccggtcgc
    12661 ggccatctcg agcatctcct cgtaggacac cacgtgcagc agccgcgcgt tgggcacgat
    12721 gcgcgggtcg gcggtataga cgccgtccac gtcggtgtag atctcgcacg cgtcggcctt
    12781 gagcgcggcg gcgagggcga cggccgtcgt gtcggagccg ccgcggccga gcgtggtcac
    12841 gtcgagcgcg gtcgaaacgc cctggaagcc cgcgacgatc gcgatcttgc cggcggcgac
    12901 ggcctccttg atgcggtcgg ctctcacgtc gacgatcttc gcgcggccgt gctcggtgtc
    12961 ggtgatgatg cccgcctgcg acccggtgaa cgacgcggcg ctcaggcctt gatcctcgat
    13021 cgccatcgcc agcagcgcca tcgagatgcg ctcgccagcg gttagcagca tgtccatctc
    13081 gcgcggcggc ggcttccgtg cgacctgatt ggcgagcgag accagctcgt cggttgtgtg
    13141 gcccatggcg gacacgacca cgacgacgtc gtggccttca cgccgggtcg cgcacacccg
    13201 ctcggcgacg atcttgatcc gctccgcgct gccgaccgag gtgccaccga acttctggac
    13261 gatcaggctc atcggaggtt ggttgtatca ggccgggagg gcaggcgcga acgtgatcta
    13321 tagatcgtgt ttgccgcgtg gcagatcgaa cagcagccgc cccagcagga tgcggccaac
    13381 cggcacgccg gctcgccggt cgtcgcgggc gccttccatg cggcggatcg cgatcagcgc
    13441 cgtgatgccg ccggccatgg cgaccagcgc gccggtgtag ccgaagatcg cgacgaaggc
    13501 cggaagaagc gcgaacccga tgctggtgcc gaacccgccc ttgcgggcca cggtgcccgc
    13561 cagcgagatg atgccggtcg cgaccatcgc cttcggggtg agcaccagcg tcacgcctgc
    13621 tgccgtggtc aggccccgcc cgcctgctcg ccggaagtac ggcggatacg aatggccggc
    13681 caccgctgcg aggccaatcc atgcgagcgt aaccgcgtcc tggtcggtcg ctcgcgcaac
    13741 cagcgccgga acgaagccct tgaccatgtc gagcacgatc gcgaggatgc caagcgcgcg
    13801 cgacatcttc gagacgacca gaaagtgcgc gtcgccgggc gattcgtccc ggcgcatctg
    13861 cgcgatgacg tcgcggcggc ggccggccag cgcaaccaga tacggcgacg gcagcgtgcc
    13921 cgccgcatac gcgatcgcga tccaggcgag gcggctcatc cggcgtgcat cctaccggcg
    13981 gatgcggctg ctagagtcgc gtgcggggcg ccgagaaccg ggcgtccgca cgcatggatt
    14041 ccgacgctcg acacactccc acgccaccgc cggaaacggc ggccactgtg ccgacggccg
    14101 cgggcgagac gcagcccgga ctttccttcg acggcttcaa ggccgtgatg agcaaccgca
    14161 gcttccggat gctgttctgg ggtcaggccg tatcggcgct cggtgactgg gtcgggacgc
    14221 tcgccttcat cgccgcggcc gatcagctct cgggcggcaa cgcgttcgcc atcaccggtg
    14281 tgctggttct tcggctggtg ccgacgctgt tcgccacgcc catcggtggc gtcgtcgccg
    14341 accgcttcga tcgcaagcgc atcatgatct ggtcggacat cgcgcgcttc gcggtgatcc
    14401 tcgccacgcc gttcgtcccg aacctgatcg cgctctatat cttcgccttc gtccacgagt
    14461 gcttcagcct cgtgtttctg cccgcccggg acgccacgat cccgaacctg gtcccggccg
    14521 accggctcga gccggctaat gcgctcgtga tggggtcgtc gttcgcgggc atcccgctgt
    14581 cggggcccgt ctacggcgcg atggccgcga tcgcgctgca cttccccgcc gtgccgaccc
    14641 agtcgcgctg gctcgagcat ccctgggggc tggcgttcgt gttcgacgcg ttcacgttcc
    14701 tgctttcggc gtggttcatc gcgcgtgtca ccatgccgcc gcgtgagcag cgcgacgggc
    14761 aggcctccga gcggttcggg gctatgctgc gcggcggaat cggctacgtg cggtccaagc
    14821 cgattctgat cggcttgtcg ttcgcggtca gcctggggct gctcggcggc ggcgtgctgt
    14881 tcgccaaagg catctcgtac gtcaaagaaa cgctcggcgg caacgacgtg gcgtttggct
    14941 acctgatggg catcttcggc ggcggcatgg tgctcggctt cgtcggatcg cagttccaca
    15001 gcgaacgcgg cgcgatgtgg acgctgcgcg gcgctctggt cgcgatgggg ggcgtgctca
    15061 tcaccatggc gctgattccc gcactgtgga tggcctacat catggcgtcg gtgttcggat
    15121 tgaccttcgc gctcgccatc atcatcggca tgacccaggc gcagctgctc acaaccgacg
    15181 agatgcgcgg ccgggtgatg gccatcgtgc acatcctggt acggctcgcg cttctggtcg
    15241 gcgcggtgat ggcagccggc atcggggcga tcttcaaaga cggactccgc gttccgggcc
    15301 tgaactatcg agccgactcg aaccaggtcg cgctggcgat cgcaggcgcg ctgatcgccg
    15361 ccggggtcgt gggcgtacgc ggcaagcgag aacgttcggg ctagtcctcg cccattcgtg
    15421 ggatcgcctc gctttgtgcg cctgccggat cgctcaaacc tcgcggcggc cttcgagcgc
    15481 gcgaccgagc gtgacctcgt cggcgtactc gaggtcgccg ccaaccggca gaccgctggc
    15541 gatgcgggtg acgcgaaccc cgagcggctt gacgaggccg gcgagataga tcgcggtcgc
    15601 ctcgccttcg acggtggggt tggtcgccaa gatgatctct ttgacctcgc cgccgctgat
    15661 ccgtcccagc agctccttga tgcgcagatc ttccgggccg atgccgtcca tcggcgagat
    15721 gtgtccgccc agcacgtgat aacggccgcg gaactcttgc gtgcgttcga ccgcaatgat
    15781 gtcggcgggc tcttccacga cgcacagaat cgtgccgtcg cgacgctgat cgcggcagta
    15841 ggcgcactgg gggccttcgc tgacgttgaa gcaggtgccg cagatcgcga tccgctcctt
    15901 cgcggccagg atcgcctcgg cgagcgcgcg cacctcttct tccggcgcgc gcaacagatg
    15961 gaaggcgagg cgctgcgctg acttggcgcc gatgccgggc aggcggcgca tcgcctcgat
    16021 cagatcctgg accggtcctt cgaacacgct agccctcgat ctcgtcgatc acttcggcgc
    16081 cgagcgcatc cttgagcgcc tcgaccggat cgaccggcgg cccggattcg gccgtgggcg
    16141 ggtcgggcgc gggcggcggc gcatcggatg ccaccgttgc gcgaatgcgc accggcgtgc
    16201 cgaacaccgc gttgaacgag tccgcgaatg cgcgcgcatg cttgagctcg gcgatctgcg
    16261 aggcgtgata gtcgaacttc gcctccagat ccagcgatcc gttcgcgtag ccgatcggcc
    16321 gcatggccgc cagcagcgcg tagagctgct tggagcgctt gcgtgtctct tccagcagca
    16381 ccggccagtc gcgcgagacg cgatcgaagt ccagcgacgc ggcgatctcc ggatcgggcg
    16441 cgggcaggtc gtcttcggcc ttgggcggct cttgcgcgcg ggcgcgagcc gaacgcttcg
    16501 gcgggatggg ccggcgctgc ggagctgcgg gcggctgctg ggcaggagct ggggcggcat
    16561 ccggcgcggc ttcctctggg gacgccgcgg ccgcagggcg gctttccagc atgcgttcga
    16621 gacgctcgat ccgggacagc agcgcttcgg gcgacgcgtc ggcgtccggc aatgcggcgc
    16681 gaacgacggc cagctccagc gtcaatcggg gcggcgcctg ctggcgcatc tcggcttgcg
    16741 cgtcggcgag cagcttgagc acgtgcacca gatgtgccgg ctggaatcga tcggcttgcg
    16801 cgctcaaacg cgcacgagac tcgtcggtcg cgtcgatcag gccgccgccg tcgggcacgc
    16861 gctgcaagaa cagcagcccg cgaaaatgct cgagcgcctg ccgcgtgaac gtgcgcaggt
    16921 cgtcgccttc gtccaccagc cgcgcgagca tgcgaagcgc cgtgcccgcg tcgcgggcgg
    16981 cgagcgactc gccgaactcg aacagcgtct cgaccggcgc cgatcccagc aggtccaccg
    17041 cgcgctcggc cgtgaccgtc ccgctgctgg cgagttggtc caaggtcgag agtgcgtcgc
    17101 gcatcccacc gtcggcgtgg cgcgcgatga ctgccagggc ggcgtcctcg gcctcgatgc
    17161 gttcctcggc gcagatgcgc gccagatgcc ggtggatgtc gtcgccgctg acgcggcgga
    17221 agtcaaagcg ctggcagcgc gacttgatcg tgccgatgac cttctcgggc tcggtcgtgg
    17281 cgaagacgaa caccacgtgc gagggcggct cttcgatcgt cttgaggaac gcgttccatc
    17341 cctgggtcga gaccatgtgc gcctcgtcca cgatgtaaat cttcttgcgt gccagcgccg
    17401 gcgccagcaa aacgttgtcg cgaagctcgc gcacatcgtc cacaccgcca tgcgaggccg
    17461 cgtcgatctc gatcacgtcc agcgacgtgc cgtcggtgat cgagcggcac gcgtcgcact
    17521 tgttgcacgg ctcgggcgtc gggccctgct cgcagttgag cgctttcgcc aggatgcgcg
    17581 ccgtcgaggt ctttccggtg ccgcgcggcc cgctgaacag gtacgcgtgc gcgattcggt
    17641 cttcgcggac ctggccttgg atcgtgtgcg caacatgctc ctggccgacg acatcggcga
    17701 aagtctgcgg acgccaccgg cgatacaggg caacctggct caaagacgac tccatacggc
    17761 tcgggttgga aggaaggcca ttctagaaga tcccggggac cctcactcgg ccgaggcggc
    17821 cgggttgacc accgtcaggt tcggaaagcg ggaaaagtct cggtccgcgg tccagagctc
    17881 tctcacgccg tgggtggtgg cgatggcggc cacccgcgca tcgtgcacct tcggacccga
    17941 agtgcgtgtg cgtgacaacg tctcccgcaa cgccggccaa tgatctccgg tctccgcgag
    18001 cagcgtcagg ccgggagatt cgatccacgc ctcgacctgg tcgagggcct gcttcagcgt
    18061 tgtcggtttt cgccagatgc gagggttggt cacgatcgaa aggaactcgt ggacgcaggg
    18121 ccaaggaatc gcccacggcc cgccggattc ggccagcatt cggacggcgc gcgaggcggt
    18181 ccgatgaaac tcgctatccc ttcggtgtgc gtagacgagg atgttggtat cgaccgcgat
    18241 caaccgccgc gccccgcgta tgcggcctgt cggatacgcg cccagtcctg atcggcgaac
    18301 tcaggctgca ggccggtgcc gtccacactg gcgtcccgca gccggaaaga ctgcttccgg
    18361 cttcgctgtg cgatcacgtg gcgcaaacct tgttccacca gttcacgcaa cgtggttcgt
    18421 tccgcggagg cgagcttctt tgcctcggcc aaaacagcgt ccgcgatctc gatggtggtt
    18481 ttcatacggg tcaccatatc acaagacccg tacacccata tcactcgggg gaaaaaagaa
    18541 gagagccggc ccctcaaccg aaaggccggc tcccagttgc cgcgctattc cgcgcggtcc
    18601 ttcgtgggtt cggtttccgg agcgactttg tcccgtgcgg ctctccgccg gtccttttgc
    18661 tccatcaccc gttccttcga agcgggcggg tccttgccgg atctgtcgtt cggcatcgga
    18721 actcacctcc gcaacgatga ttcccgggcc agcgagcgcc ctaatcctgg tcagcgagcg
    18781 aaacttttct gaaagcaaag ccggcacacg gagagggccg ggccgatccg caacacccac
    18841 cagtgcctgc ttacagctgc tgccttccgg ccctgactgg gttcacaggt tggcgtcgcg
    18901 cggggacccg accctcaccg gcgtgccggc aaggagccat atggtaccgg gtttgcgacc
    18961 tggcggagga ggtgggattt gaacccacgg gacccttgcg agcccacacg ctctccaggc
    19021 gtgccgattc ggccgctctc gcactcctcc gggcggggat tgtacgggtc gtgggtccgg
    19081 cgcggcgagc cggggcacaa agcgaccctc gccgcatccg atgccggacg cggcgagggt
    19141 cgccgtggcg gagagggtgg gatttgaacc cacggaaccc ggttaagggc tcagcgcatt
    19201 tcgagtacgc ccgattcggc cgctctcgca cctctccgcg cggcagtgta ccggggcggg
    19261 cgcgaaggct ccaacggatg aaggccgttt aacggcgggc tgcgaagaat cgccgcagga
    19321 gttgcccgca ttcggcggcc aagaccccgc cggtcacgtc catccgatgg ttgagccgcg
    19381 ggtcgcgcac gacgtcgtga agcgagccgc acgcgccggc cttggggtcg atcgctccga
    19441 acaccagccg cgggatccgt cccagcacca tcgcgccggc gcacatgaag cacggctcga
    19501 gcgtcacgta cagcgtcgcg tcttcgatgc gccacgaccc ggatgcggcg gcggcttcgc
    19561 gcagcgcgat gatctcggca tgggcggtcg gatcctggtc gcgttcgcgc gcattgtggc
    19621 cccggccgat cacgacatcg tcgcgcacga tcacgcagcc gaccggcacg tcgtcgtggg
    19681 cgagcgcggc ctcggcctcg gccagcgcca tccgcatgaa gtcgtcgtcg ctcacagctc
    19741 gatctcggtg cggtcgatcg ggtcgaagcg gtgcatccac agatccagcg cggtccagat
    19801 cgacttcgag aacggataca gcaggatcgg cagcaccagg ttcatcccga ccgcggtcag
    19861 tgtgatcgcc agccacggtg gatccggcca ggcgagcgcg gcccaggtga ccagaagaat
    19921 gccgaacgcg atctcggtca cgaccaggtt aatggccatc gcgccgacga agtgcgcctg
    19981 ctcgcgtgcc agcttcagat gacaccgcgg gcagcgttcg cgcatcgtga accagccgtc
    20041 gaacaggttg cctcccccgc atcgcgggca gcgcttggcg agcccgcgga cgatcatctg
    20101 cccgccggag ggctcgtagg gagggcctgg cgtggtcacg gcgatcagcc tacgcctcgt
    20161 gccttgacgg ggcaccccgg tggcgcgagg atcgcggtgg ctcgaagggg gaggacgcgt
    20221 gagcgcgccg atactgatct ttttacacgg gccgatggcg ggcaagcgga tcccgatcga
    20281 ccgggacgag ctgaccatcg ggcgcagcga caccaatgac gtcgtgctcg acgacaagct
    20341 cgtgtcgcgg ctgcacgcga tcgtgatgcg gcgcggtgac gtggtcctgg tcgaggacct
    20401 cggcagcaac aacgggacct acgttcagga cgagcggctc cacgcgatcc gccagctgca
    20461 cgacggtcag acgatcacga tcggaaccag ccgcgtcatg ttcgaagacg cctcggcctc
    20521 cggcgaggag acgacccaga tcgccgcggg tggcggcatg gcggcgccgg cgacgttcac
    20581 gcagcggcag atgcaggtgc tccggctcct ggcccgcggc ctgtcgaaca agcagatcgg
    20641 cgagcggctg ttcgtgagcg agcgcaccgt caaggcgtac ttgtcgagca tcttcgacaa
    20701 gctcgaggtc gggaatcgct cggcggcgat tacggcggcg ctgcggcttg ggttgatcga
    20761 cattccggga gacgccgacg cgtccgcgga atgacgacga cggaccattc aggcccgcga
    20821 aggtcctgcc gaaactcggg gggaggaaca gcgctcgaaa gtgttgtggt ccgagggatg
    20881 aagccaccgc gcgagggatg aaggaccggg cagctgcgca gacctcataa cattgctcgt
    20941 atcggggccg gccctgccgg ccccgttttc tgtgtccgtc gcgttcgttg cgccggatag
    21001 gtccgaacat ttggaggaaa tcggcgccga gcgaaaggga ttatcccgca gcgggcgaac
    21061 aaccgcgtaa cgcatcgccc taccagggca ggagaggggg ttttcacttt gaagaagatg
    21121 cttgcgctgc tgatgctcgt gttcgcgatc gccgccgcgc cgatggtctc gtccgcggac
    21181 gagtgcggcg agtcggagat cgcccgggtg tgcgtgaccg acgacggtgt catcgtcgtg
    21241 gacggtgccg agggcaacga ggacccgctc gacggctacg tcaccatcga cgtccagaac
    21301 ggcgagatgt gtgccgacag cgacgggaac tatgacgatc ccgacgcgga cccgaactgc
    21361 tactcgctgt agttcgaatc gtactcagag aaggcgggcg gttgcccgcc ttctcgaggt
    21421 accgggggga cccgcggtgt agccttgcct catgcaacgg cttgtcgcca tgcccggcga
    21481 gtattcgctg atcgcgacgc tgtctcccct tcggcacggc ggatacgatc cgaccgtcac
    21541 gatcgagcgg aacgcggtga cccgcgcgta ccggatcggc ggcgagcccg tcaccgtccg
    21601 ttacgagcgt gttgccgacg gggttcgcgt caccgcgtgg ggacccgccg gcgaaaaggc
    21661 cctcgacgag gctccgtccg tgctcggatc cttcgacgat ccctccgcct accggcccgt
    21721 gcatccgatc ctgcgggact tgcaccgggg ccggcccggg atgcgtatga cggcgacgcg
    21781 cgcggtgttc gaagcgctcg tcccgtcggt gctcgagcag aaggtcaccg gccgggaggc
    21841 gacgaacgca tgggctcgct tcgtgcggcg ttccggcgag gcggctcccg gtcccttcga
    21901 tctgatgctc gctccggttc ccgaggcgat cgccgcgatg ccgtactacg tctggcatcc
    21961 gttcggtgtg gaacggttgc gcgccgagac gatccgccgc gcgtgcgcgc gggccggtcg
    22021 cgtccgcgag ctcgccgcga tggacgcagc cggcgcgcgc cgtcggctgc gcgcgctgcc
    22081 cgggatcggc gtgtggaccg ctgccgaggt gacgcgcgtc acgcacggcg acgccgactg
    22141 tgtcagcatc ggggacttcc acaccaagaa cctcgtgtcg tgggcgctgg ccggcgaggc
    22201 ccgcggaacg gacgagcgca tgatggagct gctcgagccc gaggcgggcc accgcgggcg
    22261 cgcggttcgg ttgctcgaga tggccgggat ggcagcgccg aagttcggtc cgcggcagcg
    22321 gatcagacgc atcgcccaca tctgacggcc caactctggg cctgggctac tgcgagaggt
    22381 cgtcgtagcc ttcggtccga ggcaagaaag ggggccgcaa tggcgcacaa gaacgtcgag
    22441 atccttcggc gcacggacga gacgatgcag gccggggacg tcgaggggtt cttcggcacg
    22501 ttcaccgacg atgtcgtcat ccacgttccg ggccgtcact ccttcgccgg aagctacaag
    22561 ggcatcgacc agttccgagg actgttcgag aagttcatgc agcacgccgg cgactactcg
    22621 ttcgagaacc acacctacct cgccgacgac gagcacggcg tcgccatgca gcactccacc
    22681 tacgagaagc agggccgccg cctggcgatg gacgaggtgt tcatctgcca cttccgtgac
    22741 gggaaggtct ccgagatgtg gtatctcgcc ggagatcagc aggcgctcga cgagttcctc
    22801 ggctagcggc tgacggagcc gtccgcggga cggggatcgc gggtcgcagc acgccgcgcc
    22861 gatgggtggc acactgcgcc gatgccacga cgcacccggt tcgcggacgt tctgccggcg
    22921 attcgccgtg ccttcggggc cgcgcccgac catgccggca ggctcgacga aggtgcctac
    22981 tcgccggcgg gccggtggtt ggtgcgattc gcggacgggc gcagcgcatt cgtcaaggcc
    23041 gagtgggagg cctcggacgg tcacgggacc gccgtcgagt acctcgtgta ttcgaacgtg
    23101 tcggccccgc cggttccgcg gctgatcgcc tacgaagccg gctcgaccga ggcgccacgc
    23161 gtgatcgtca cggaggacct gtcccacgct cggtggggag cgccggtgac gcgcaaggac
    23221 gcgacgctct tcgatgcggc ggttgctgcg ctcgaagggg tggccgcgcc cgaaggcatc
    23281 cgggacatcc cgtttcggcc gcagtgggac gctctcgtcc gcgacccggc accgctcatc
    23341 gcggcgggcg tctgcgatgc acgctggatc gagcgatatg gcgagcggct gtccgccgcc
    23401 gaggcgacgg tcgatccgtg cggctccgga ctggtgcact gcgatctatg gctgcagaac
    23461 tggtgccggg cggaccgcgg cgtggtgatg gtggattggg ccggcgcggc tcgcggctcg
    23521 ctcgacgtcg ctcgggcgtg gggcgaggcg gcggtgcgcg ctgcgggcgg gccgagcaac
    23581 gtgatcgtcc cgaaggggaa tgcgggctgg gccgcgttca tggccggcct tgcggcgcag
    23641 ttcgccgcag aggatcacgg cgagcccgcc tcgcctcgtc tcgtcgagac gatccgtcgc
    23701 gaggcggcgg ctacgctcgc gtgggcgtgc gaagagctgg agatcgagcc ggtcacgcat
    23761 gcccccggat tcgatcccgg gggcccgtgg cgtccttagc cgcgcgttag gtccacgcgg
    23821 gcaggtgatg cagcggattg accgtgcggc cgttccggta gaactcgaag tgcaagtgcg
    23881 ggttggtcga gtttccggtc gatccgcacg tcccgacgac ttggccgcgc tcgacccatt
    23941 ccccggcgcg aacgcgcagg ctccacatgt gcgcgtacag cgtcttagtg ccgttctggt
    24001 gctgcaggat cacggcgcgg ccgtagaccg gcatgttgtt ggacgcgata acgcgtcccg
    24061 gcgcggccgc gaagatcggc tcgccgccgg tcttcgagca caagatgtcc atgccgttgt
    24121 ggaagccgcg gcgacggttg ccgaaatggc tggtgatcat gtccacgtgc ggccacgaaa
    24181 ggccgtagcg gctcaccttc gcgcggatgg ctgcttcgcg acgctgcttc tccgcctcgc
    24241 gacgctgctt ctccgccagc cgctccgctt cgatgcgcgc tgcctccatg cgctggacgg
    24301 cctcccaccg cgcttgctct acggcggcga cgacatcggg agagatctcg ttcgactgcg
    24361 aggacacacg cccgccgcca ccccccgaga atccgatgtt ggccccaaac gccgccacgc
    24421 ccgtaatcgc ccccacgaac accgtcagaa tcgctaccca ccatttcgtc gcacgcaaga
    24481 caagacctca cacggtagtt ggcttgatga agaacgttca tctgggtctc gacacccgac
    24541 ggcatgccct caagtgggcg acccgaacat tcgctttgat tgatccggat tgatcacgta
    24601 cgcagttcgg gaacgtgata cgaacggtct ggttcgggtg aggcgccgcc cggtgcctcc
    24661 gcgaagggcc gtccgctatc ccccattcgg ataaacgcgc atggtaccga ggtgtttcgt
    24721 gatccgctcg cgaaacgccc atatttttcg aggaaattga gcgacggttg cttacgaagc
    24781 ccggaatgct tgatcgcgat acattattgg ttgctttcga ggaggcccgc gatgctgcac
    24841 cgcaagacga ccatgatcga accagagcga gcactgcccg gacgtgctac ggcgttgtct
    24901 gttcccgaac ggcatcacgc gctcggcaca ccgcttcaag ggccctggcc ggacggaagc
    24961 gagctcgcca tgttcgcaat gggttgcttc tggggcgccg agcgaatttt ttggcgcatc
    25021 gacggcgtgc actccacttt cgtcggctat gcaggcggct acacgccgaa tcccacctac
    25081 gaagaagtct gctccggact gaccggtcac gccgaggtcg ccgtcgtcgt gttcgatcct
    25141 gcccgcgtgt ccttcgagac gctgctcaag gcgttctggg agaaccacga cccgacgcaa
    25201 ggcatgcggc aaggcaatga cgtcggtacg cagtaccgct cggcgatcta cacgtacggc
    25261 gacgagcagc acgacgccgc caagcggtcg cgtgaccgat accaagcgct gctgcgcgac
    25321 aaggggttcg gcgacatcac caccgagatc gcgcccgcgc cggagttctt ctacgccgag
    25381 gactaccacc agcagtacct atccaaaaat ccgaacgggt actgcaacca cggtttctgc
    25441 caggtcgagt actcctgatc cgcccggcga tcaagggagc aagcccgccc cgggtttagg
    25501 atcggccgcg atggccggcc gcgatagcgt catcgacctg gtgcgctcgg gcgcgaccac
    25561 gacggtggtc gtctaccact gggtcttcac catcatcgtg tggcgacccg acggcccgca
    25621 tgccgacaac cccatcggat acgtgtcggg cctgtggttc ctgacctgga tcctgcaggt
    25681 gcttcccttg ttcttcatgg ccggcggctt cacgcattcg cttgcatggg cgaagtatcg
    25741 cgagacgccg aaggcatggt ggcggttcgt cgcgcgccgc gccggacagc ttctcgggcc
    25801 cgcggtgctg ctcgtcggtg tcgtggtcgg tgcggcgctc acggtcgctt cgctgcgggc
    25861 cggctcggat tcgtggttcg cgcgcggcgt gatcttggtg ctgtcgcctc tgtggttcct
    25921 cgtcgtctat ctggtgctgg tggccaccgt tcctttgtgg gaccggcttc accggcgctt
    25981 cggcgagctc gtcccgctcg ggttggctgc gctggcgatg agcgtggacg tgcttcggtt
    26041 ccgctacgac gtgggctgga tcgccttcgc gaacctggtg ttcgtgtggg cggcctgcca
    26101 tcaggtggga tggtcgtggg accggctgag cgcggcgccg aagcggttcg ggcacgcgct
    26161 gatcttgatc gggttcgccg cgctggcagg gctgacgaac atgggtctgt atccccgatc
    26221 catggtcggc accacctcgc cgctggaccg cttctccaac atggggccgc ccacgctgcc
    26281 gatcatcgcg ctgctgacct tgcagctcgg cctggtggtc gccaatcgcg atcggctggc
    26341 cgcctgggcc gagcggcccc gcatcgcgcg ccgggtcgca tggatgagcc gcaacgcgat
    26401 gccgctattc ctgtggcact cggtcggctt cgcggcgttc ttcatcgcga tgcgttcgtg
    26461 gaccacggtg cccgagtccc cgacgctcgt gtggtggctg acgcggccgc tgtggctgat
    26521 cggtccggct ctggcgacgc tgccgctcgt cgccgcgagc acacgcctgc tgcggccgtc
    26581 gggcgcggtg acgggcgcgc gcaagcgcac ggccgtgccc gcgtgatccc gtgggcgccg
    26641 cctggctcgc cgcagcgctg gtcgcctaca acgccgcggc gaaccggtgg aagccgtttc
    26701 attcatgggc ctacctgccg gccaacctga cgcttgcggc cgccgtcgtg tggctcggag
    26761 ccgcagcgtt cgatctgagt gccggcgaga tgggctttcg cggcggctcg atccgggtgg
    26821 gcgccgcgat cggggtcgcg ctcggggtcg cggcgatggt gccggtctac gccgcgctcg
    26881 gcttcgagcg agggcgcgcg ctgctgcgag accggcgtct gtccggcgtg cgcggcgcca
    26941 aagccgcgtt cgtgctgctc gtgcggatcc cgatcgggac cgcgctcgtc gaggagacgg
    27001 tgtttcgcgg cgtgctgctg ggcagcgcga tgagccacgg caccgcgcag gcggtcgcgc
    27061 tcagcagcgt cgcgttcggt ctgtggcaca tcgttccgac ctggatcctc gcccgcaaga
    27121 accggctgaa cgtcgccgtt gtgcccgcgg gcgtcgtggt gaccggcgcg gcaggcgcgt
    27181 tccttgcgtg gctgcggatc gagaccggcg gcatcgccgc gccggtcgcg ctgcacgtcc
    27241 tggtgaactc gccggcggcc gcggccgcga tgatcgcgct tcgcctgcct gccgctagcc
    27301 gcggcccgtc ccgttgatcg cgcgaagcga aaagccgctc aggcactgct gatcgccgac
    27361 cggcgtgagg atgcggagca ccgatcccat gcagtcggcg gtcgtcgtgt aggcgaccat
    27421 cggggtggtc gcagcgctcc agccggcggc cggcgtgaag ccgaccgtga gcacctgacc
    27481 cgcaaccgag cggaccgtca ggccggtcgc gcgaaccgag gcattggacg ggtcggtgat
    27541 cgtgagccgc gtcagcgcgc cgctcgagac cgagcttgcg tcaaaggcat gatgcgacac
    27601 gagctggatc tcgtcgatca cgccgtcgtt gttgcggtcg cccgtgaccg gcgccgccgc
    27661 gagcatgccg ggcgcgtgca ggaccgtggc cggcgccgcg cgtgccgaca gcggattctt
    27721 ggccgcgtcc acgatgttgg ccgcatagga gctcacgacc gtgaacgtgc acttggcgag
    27781 tgtcgcgccc gccgggttct gcgcgggacg tgctgcgaac acccagccgg tggtggacgg
    27841 caccgaaccg tgcttcatcg tcacgatgat ctgatcgccc gatgc
//
LOCUS       DATLFO010000166         9182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_84806, whole genome shotgun sequence.
ACCESSION   DATLFO010000166 DATLFO010000000
VERSION     DATLFO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84806"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1013)
                     /locus_tag="VM841_06565"
     CDS             complement(69..1013)
                     /locus_tag="VM841_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL89881.1"
                     /translation="MSAVPRSVRAAALAVTLAMVGVACGGGSGGGDLTGTVSVDGSST
                     VFPISQAVAEEFNVEQPDVKTSVGVSGTGGGFKKFCSGETDMSNASRHIKDSEIAECK
                     KNGIEYLEMTVAIDGLSVLVNPKNTWAKCLTTEELKKIWSPEAQGKITNWRQIRPEFP
                     DKPLKLYGPGTDSGTFDYFTDAIVGEEGKSRSDYTASEDDNVLVQGIAGDENALGYFG
                     FAYYEENVEKLSIVQVDPGSGCVEPSRQTINDGTYKPLSRPLFVYVAKKAAARPEVEA
                     FVDFYLENVNELVESVGYIDMPAAMLAEQKSKWEAFKA"
     gene            1166..1594
                     /locus_tag="VM841_06570"
     CDS             1166..1594
                     /locus_tag="VM841_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVL89882.1"
                     /translation="MAIHDAVSHRLQGVGQRYTASRRAIVTALAAAPHPLSITELVAT
                     GRGLAVSSVYRNLGVLEEAGVVHRIDTSHEFKRYELAEDLTEHHHHLICTGCGRVQDF
                     TASPGLERQTEQALAGVAGKSGFTVRGHRIDLVGLCRNCA"
     gene            complement(1596..3569)
                     /gene="ftsH"
                     /locus_tag="VM841_06575"
     CDS             complement(1596..3569)
                     /gene="ftsH"
                     /locus_tag="VM841_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVL89883.1"
                     /translation="MFVKLVSAGSARVVRAVQAAFAWVRSIGIVPRALTVSVVVMLVA
                     YAAVLASLAPIERGRQTTVDRVRSLAESETIERATFYDEDARAVIVTRHGGTWWAAYP
                     KSDAQTADLIDQLSRGGARILVDQQSEKARLRFLAQFLFPMLILAGLFGLVFVVISGK
                     GGASEFQAFSKFSGGRGPGDAITFANVAAAREAIIELQEVVDYLQRPERFATVGALAP
                     KGVLLVGPPGTGKTLLARAVAGEAGVPFFSLAGSDFVESLVGVGAARVRDLFRQAREA
                     APAIIFIDELDAAGRQRGAGMGQGNDEREQTLNAMLVEMDGFSASSGIVVLAATNRPD
                     ILDPALMRPGRFDRQVVVDAPDVEGRIEILELYAKKRPMDAEVDLPRIARQTPGFTGA
                     ELSNLVNEAALLAVRADRNAITTRDLEEAVDRVIAGPERRSHVLSEDEKRLVAYHEAG
                     HAVVAAGVGMRTGVQKLSIVARGRSLGHTTTYQISDRLVLTRDDLVRQLVTVMGGVAV
                     EQAMLGVTTTGSEGDLRQATGIARAMVATFGMGEALGRVAVGQKSGEVFLGRDFTKMN
                     DVAPATMEAVDQEVRSMLDDAEARATEIIRSNRDGVERIVSLLMERETLSGPDLAAVL
                     DGIVAAPRPGRRATGRPRRGRPVVDVRDAVRAR"
     gene            complement(3587..7720)
                     /locus_tag="VM841_06580"
     CDS             complement(3587..7720)
                     /locus_tag="VM841_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89884.1"
                     /translation="MIVAITSGALAGIAGTRSPRPVSSVGRDLGRLHGPRMDPVLAPA
                     RVARVVAVPGSADAEAWAYGHTHWRNPGWSQTDPQGQAVFLSYRRGRGWSIEQPLVDP
                     NGNPTSPGVAAFDMVEGGEGYGVGGTGLIFHRPAGGRWQVHPQSGAVATVDLTAISLA
                     TDAAGTYGWAVGSGPTFLRLTDGTWQHDLGSVRGAGGTGDILGNVVGVATIDRETAWA
                     VAATPRQLVLYRRNASEGWQRVQTGKPLFDVAPSLHANAGGAPVVNQFATGAAIAAAG
                     RAVWVTGALQPVDGQRYVNQRGPGSDPQRPFALRIDQNGSFTTYCAPVYQLSSGGVTG
                     TNDLCEHPFPFSVGSLPALDGLSNGDVFAGGAGVFRYAAGRWTREPNVAGLVSSLSFG
                     SATEGWVTSFGNRTAEGGSLAASTSPTLGHWTVDAATPSMLRRWPHPSRRTLEGIVLA
                     PDGEAIAVGGGGDIARFEPGVGWDMMPSPTDVALHDAAWPAPGQAWAVGDRGIILRFD
                     GTRWRHDAASRRLTKEALYALAFREANDGIAVGARGTILRWNGSSWRADPAGGKTVDV
                     RLHAVAYVGSETIVVGEKSTILIDGGDGWKQDSTAPERFKTLQEGRIPNLFTVVGLDD
                     GRAFVGGELSILLERVPGGRFERTSSFPVLNGSILTLAATRTNGRLRLLASVATEPSG
                     RVTKYGGSGLIEPTGWLFASDGDTWSASGDQRVAETGTEVDAPVRRDAVYDIAVDASG
                     RGFAVGGYPADLADEDGHLSGAASGSIWRLDTRGMPEPAPNTARVPVRAESGSIGFAF
                     LADTACVSGLCAAASGTGARADEIVARALADVEDAAASGEVRFVTFGGDFRRLGIPDE
                     LDVIGRMLDTLPVPSFAAIGDQDLFGGVDAAGESVLASNGYFLSAFADRPAPWGHAPP
                     HPSIRPLSAPGEPVADSTQARTHYAFDYMIDGRPALRMIVLDTSRTAQEIAFQNPQSD
                     QDSAFARPLLAEAAALSIPSMVVMHQPLLASVGTGARTGTLTAALAGTGVNGVLASHE
                     RVNRLIAYADPVQPFPVAILGSTGGALNGSWNPEAGAYHAWFHVSVPLDGDAHPRIRS
                     IPVLESVALSAPGGRTAGAGGTLRFLGLGRIPDVGGGHIASGGDPTSDRAQYLSFPFA
                     RPCRGPLQNPGNAACTGADVLVPDFEFSCEDRRVCVFVAEDPVRPGTPLINAAGTMEA
                     SAASGLMCALRPGSTFVRLRAGVVQTRMPVRVTGGDGACVDRPVVDASAPAVVPASDP
                     RQAPSPVDRDAPPSILKPKSFDAAAIPALAPPPIQPAPAPPGGGAPKYEEEREASVEK
                     AEMTALDPAAMFAAAFALAVGLALRVRPRGRAAPARRTAAPQAYAGAPPDVRRWLRP"
     gene            complement(7828..9087)
                     /locus_tag="VM841_06585"
     CDS             complement(7828..9087)
                     /locus_tag="VM841_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVL89885.1"
                     /translation="MRKSIVRLLIAAATALPMAFGGLAAAETPDSPLGAPLVGNGRFC
                     SVSSPIQGTGATFANNAHQNVFIPEYNADCGSGSGNVSYTSTGSGAGKFAAIEHTHWF
                     GMSDEPLSTDETLCATLDLGFTAPANGTCRLSPGRNRVSPIHHIPLALGSITVSYNVS
                     FPGCTFGQKALKFRSEVVGSIFSGVITRWNDPLLAVDNPGLANAACNKPIKIAVRQDG
                     SGTTYAFKDFLSKRNPQFQVYKQNQLNTAWPAQDLGLNTPLRGNGNGGVADQVKNNAG
                     AIGYVELSTAKSKGLTFGLVDGPHRQFLAPDQGNGANCEASATGATHPPSTLSPGWDA
                     VSITDSPNPLGYPVCTFTYALVYNNLKSAFGSAFIATKARTLADYLLVALDADTQNKL
                     NAVGYGRMPASFIAVAQAGVATITDQA"
BASE COUNT         1480 a         3218 c         3036 g         1448 t
ORIGIN      
        1 cgctttgatc tgccatccgt cccccgtccc ggcggcgggc atcttagacc aacgcgcccg
       61 atggcgcgtc atgccttgaa ggcctcccac ttcgatttct gctccgcgag catcgccgcc
      121 ggcatgtcga tgtagccgac gctttcgacg agttcgttca cgttttccag atagaagtcc
      181 acgaaggcct cgacctccgg gcgcgccgcg gctttcttgg ccacgtagac gaacagagga
      241 cgcgacaacg gcttgtaggt gccgtcgttg atcgtctggc gtgagggttc gacacagccc
      301 gagcccgggt ccacctgaac gatgctcagt ttttccacat tctcttcgta gtacgcgaag
      361 ccgaagtagc cgagcgcatt ttcatcgccg gcgatgcctt gcaccagcac gttgtcgtcc
      421 tcgctggcgg tgtagtcgct gcggctcttg ccttcttcgc cgacgatggc gtcggtgaag
      481 taatcgaagg ttcccgaatc ggtgccgggt ccgtacagct tcaacggctt gtcggggaac
      541 tccggccgga tctgccgcca gttggtgatc ttgccctgcg cctccggcga ccagatcttc
      601 ttcagctcct cggtggtcag acacttcgcc caggtgttct tgggattcac cagcacggac
      661 aggccgtcga tcgccaccgt catctcgaga tactctatgc cgttcttctt gcactcggcg
      721 atctcgctgt ccttgatgtg gcgcgaagcg ttggacatgt cggtctcgcc agagcagaac
      781 ttcttgaagc cgccgccggt gccggacacg cccaccgacg tcttcacgtc gggctgctcc
      841 acgttgaact cttcggcaac cgcctgcgag atcggaaaca cggtgctgga cccgtcaacg
      901 ctgaccgtgc cggtcagatc gccgccgccg ctgccgccgc cgcacgcgac acccaccatc
      961 gcaagcgtta cggccagcgc agccgctcga accgaacgtg gaactgctga catgaatcct
     1021 ccttgatgat tgatccttca ccggcgacgt tagatgccgc gcgtgtcgcg ggtccggccc
     1081 gaacatgaac ggaccgtgaa cgatcggtga actctggagg cccggtcgtt tctcagtaat
     1141 tctcaactat ggctatactc gccgtatggc aatccacgac gccgtttcgc accgtctgca
     1201 aggggtggga cagcgctaca cggcgtcgcg ccgtgcgatc gtgacggcgc tggccgctgc
     1261 accgcatccc ttgtccatca ccgagctggt cgcaacgggc aggggcctgg cggtcagctc
     1321 ggtctaccgc aacctcggag tgctggaaga ggccggcgtg gtgcatcgca tcgacaccag
     1381 ccacgaattc aagcgatacg agctggccga ggacctcacc gagcaccatc accacttgat
     1441 ctgcaccggc tgcggacgtg tccaggactt caccgcgtcg ccgggcctgg agcgccagac
     1501 cgagcaggcg ctcgccggcg tcgccggcaa gtccggattc accgtgcgcg gccaccggat
     1561 cgacctggtc ggcctctgcc gcaactgcgc ctgactcagc gcgcgcgcac cgcatcacgc
     1621 acgtccacga ccgggcgccc tcgtcggggt cggccggtcg cgcgcctccc gggccggggc
     1681 gccgccacga tgccgtcgag cacggcggcc aggtccggtc ccgacaacgt ctcacgctcc
     1741 atcagcaacg acacgatgcg ttcgacgccg tctcggttgc tgcgaatgat ctccgtcgca
     1801 cgcgcctcgg catcgtccag catcgatcgg acctcttgat ccacggcttc catcgtcgcc
     1861 ggcgccacgt cgttcatctt ggtgaagtcg cggcccagga atacctcgcc cgatttctgt
     1921 cccactgcga cgcggccgag cgcctcgccc atgccgaacg tggcgaccat cgcgcgggcg
     1981 atgccggtcg cctggcgcaa gtcgccttcc gaaccggtcg tcgtcacccc cagcatcgcc
     2041 tgctcgacgg ccacaccgcc catcaccgtg accagttgcc ggacgaggtc gtcgcgcgtg
     2101 agcacgagcc ggtcgctgat ctggtatgtg gtcgtgtgac cgagcgaacg gccgcgcgca
     2161 acgatggaca gcttctgcac gccggtcctc atgcccaccc cggctgcgac gacggcatgg
     2221 ccggcctcgt gataagcgac gagacgcttc tcatcctccg acagcacgtg gctgcgccgt
     2281 tccggtccgg cgatcacccg gtcgaccgct tcctccaggt cccgagtcgt gatcgcgttg
     2341 cggtcggcgc gcacggccag caatgcggct tcgttgacca gattcgacag ctccgcaccg
     2401 gtgaatcccg gcgtctggcg ggcgatgcgt ggcagatcca cctcggcatc catgggccgc
     2461 ttcttcgcgt acagctccag gatctcgatg cgcccctcga catcgggggc atccacgacg
     2521 acctgccggt cgaagcgtcc gggtcgcatc agcgcggggt ccagaatgtc ggggcggttc
     2581 gtggcggcca gcacgacgat gcccgacgat gcgctgaacc cgtccatttc gacgagcatc
     2641 gcattgaggg tctgctcacg ttcgtcgttg ccttgcccca tgccggcgcc ccgctgccgt
     2701 ccggcggcat ccagctcgtc gatgaagatg attgccgggg cggcctcgcg cgcctgccgg
     2761 aataggtcgc ggactcgggc cgccccgacg ccgacgagcg attcgacgaa atcggagccg
     2821 gcaaggctga agaacggaac gccggcttcg ccggcgacgg cgcgcgcgag caaggtcttt
     2881 ccggtccccg gtgggccgac cagcagcacc cccttcgggg cgagcgcacc gacggtggca
     2941 aagcgctccg ggcgctgcag atagtcgacg acctcctgga gctcgataat cgcctctcgc
     3001 gcggcggcga cgttggcgaa cgtgatcgca tcccctggcc cgcgcccgcc gctgaacttc
     3061 gaaaacgcct gaaactccga cgcgccgcct ttcccgctga tgaccacgaa gaccaggccg
     3121 aacaatccgg ccaagatcag catggggaac aggaactggg caaggaaccg cagccgcgcc
     3181 ttctcacttt gctgatccac cagaatgcga gcgcccccgc gcgagagctg gtcgatcagg
     3241 tcggcggtct gcgcgtccga cttcggatac gccgcccacc aggttccgcc gtgccgcgtg
     3301 acgatcacgg cgcgcgcatc ttcatcgtag aaggtcgcgc gttcgatcgt ttcgctttcg
     3361 gcgagcgagc gaacgcgatc caccgtcgtc tgccgcccgc gctcgatcgg tgccagcgac
     3421 gccagcacgg ccgcatacgc caccagcatc accacgaccg acaccgtaag cgcgcgcggc
     3481 acgataccga tcgagcgcac ccacgcaaag gcagcttgca cggcgcgaac gacgcgggcg
     3541 gatcccgcag agacaagctt gacgaacatt cccgctcctt cggattctag ggtcgtagcc
     3601 atcgacggac atcagggggc gcaccggcat acgcctgcgg cgcggcggtt cggcgtgccg
     3661 gcgcggcccg cccgcgaggg cggacgcgca gcgccaggcc aacggccaac gcgaacgcgg
     3721 ccgcaaacat cgccgccgga tccagcgccg tcatctcggc cttctcgacg gaggcttcac
     3781 gctcttcttc gtatttcggc gcgccaccgc ccggcggcgc gggcgcaggc tggatcggcg
     3841 gcggcgccag cgccggaatc gcggcggcgt cgaacgactt cggcttgagg atcgacggcg
     3901 gggcatcgcg atccacggga gagggtgcct gacgcggatc cgacgcgggc accacggccg
     3961 gtgcggaggc gtcgacgacc gggcggtcta cgcacgcgcc gtctccgccg gtgacgcgca
     4021 ccggcatgcg ggtctgcacg acgccggcgc gcagcctcac gaaggtcgag ccgggccgca
     4081 gcgcacacat caatccgctg gccgccgacg cttccatcgt gccggcagcg ttgatcagcg
     4141 gcgttcccgg gcggaccgga tcctcggcga cgaatacgca cacgcgccgg tcttcgcacg
     4201 agaactcgaa gtcgggcacc agcacgtcgg cgccggtgca ggcggcattg ccggggttct
     4261 gcagcggtcc gcggcatggg cgggcaaacg gaaagctcag gtactgcgcg cgatccgacg
     4321 tcgggtcgcc gccgctcgcg atgtgtccgc cgcccacgtc ggggatgcgg cccaaaccaa
     4381 ggaaacgcag cgttccgccc gcgccggcgg tgcggccgcc gggcgcggac agggcgacgg
     4441 actcgagcac cgggatgctc cggatccggg ggtgagcgtc gccgtcgagc ggaacgctga
     4501 catggaacca tgcgtgatac gcaccggctt cggggttcca cgatccgttg agcgccccgc
     4561 cggtcgaccc caggatcgcc acggggaacg gttgcacagg atcggcgtac gcgatcagcc
     4621 ggttcacccg ctcgtgagag gcaagcacac cgttgacgcc ggttccggcc agcgccgcgg
     4681 tcaacgttcc ggtgcgcgca ccggtgccga ccgaggcgag caacggctga tgcatcacca
     4741 ccatcgacgg aatggaaagc gcagccgcct cggccagaag gggccgcgcg aacgccgagt
     4801 cctggtcgga ctgcggattc tggaatgcga tctcttgcgc ggtgcgcgat gtgtccagaa
     4861 cgatcattcg cagcgccggc cggccatcga tcatgtaatc gaacgcgtaa tgcgtgcggg
     4921 cctgggtcga gtccgcgacc ggttcccccg gtgccgacag cggccgaatg ctgggatgcg
     4981 gaggggcgtg tccccacggc gcgggccggt ccgcgaacgc cgaaaggaag tatccgttgg
     5041 atgcaagcac gctttcaccg gctgcgtcca cgccgccgaa aagatcctga tcgccgatcg
     5101 cggcgaacga gggaaccggc agcgtgtcga gcatgcggcc gatcacatcg agctcatcgg
     5161 ggatgccgag ccggcgaaag tcgccgccga aggtgacgaa gcgcacttcg cccgatgcag
     5221 cggcgtcttc cacgtcggcc agcgctcgcg ccacgatctc gtccgcgcgc gccccggtgc
     5281 cgctcgcggc cgcgcacagc ccgctcacgc acgccgtgtc cgcaaggaag gcgaacccga
     5341 tcgagccgga ctcggcacgg accggaaccc gggcggtgtt cggcgcgggt tcgggcatgc
     5401 cgcgggtgtc cagacgccag atcgagccgc tcgccgcgcc gctcagatgg ccgtcctcgt
     5461 cggcgagatc cgccggatag cccccgacgg cgaacccgcg gccggacgcg tccacggcaa
     5521 tgtcgtagac ggcgtcgcga cgcaccggcg catcgacttc cgtaccggtt tcggcgacgc
     5581 gttgatcgcc cgacgccgac cacgtgtcgc cgtcgctcgc aaacagccat cccgtcggct
     5641 cgatcaggcc ggagccgccg tacttggtca cgcggccgga cggctcggtc gcgaccgacg
     5701 ccagcaggcg aagccgtccg ttcgtgcgcg ttgcggccag cgtcagaatc gatccgttga
     5761 ggacggggaa ggatgaggtg cgttcgaatc gcccgccggg gacgcgttcc agcaggatgc
     5821 tcagctcgcc gcccacgaag gcgcgtccgt cgtccagccc cacgacggtg aacagattcg
     5881 ggatgcgtcc ctcctgaagc gtcttgaacc gttcgggcgc ggtcgaatcc tgcttccatc
     5941 cgtcgccgcc atcgatcagg atcgtgctct tctcgccgac cacgatcgtc tcgctgccca
     6001 cgtaggcgac ggcgtgcagg cggacgtcga cggtcttgcc gccggccgga tccgcgcgcc
     6061 acgacgaacc gttccagcga agaatggttc cccgtgcgcc gaccgcaatg ccgtcgttcg
     6121 cttcgcggaa ggccagtgcg tagagcgcct ccttcgtcag ccttcgcgag gcggcgtcgt
     6181 gccgccatcg ggtcccgtcg aaacgcagga tgatgcctcg gtcccccacc gcccacgcct
     6241 gcccgggggc gggccacgcg gcgtcgtgca aggcgacatc ggtcggtgag ggcatcatgt
     6301 cccacccgac ccccggctcg aaccgcgcga tatcaccgcc gccgccgacc gcgatggcct
     6361 cgccgtccgg cgccagcacg attccttcca gcgtccgtcg ggacgggtga ggccagcgac
     6421 gcagcatcga cggcgtggct gcgtcaacgg tccaatggcc cagggtcggc gaggtcgatg
     6481 ccgcaaggct tccgccttcg gccgtgcggt tgccgaacga cgtgacccac ccttcggtgg
     6541 ccgaaccgaa cgataagctc gacaccaggc ccgcaacgtt gggttcgcgt gtccaccggc
     6601 cggcggcgta gcggaaaacg cccgctccgc ctgcgaagac gtcgccgttc gacaggccgt
     6661 ccagcgccgg cagcgatccg accgagaagg ggaacggatg ctcgcacagg tcattggtgc
     6721 cggtcacgcc acccgacgac agctgataga ccggcgcgca gtacgtcgtg aacgaaccgt
     6781 tctgatcgat acgcagcgcg aagggccgct gcggatccga tccgggcccc cgctgattga
     6841 cgtagcgttg tccgtcaacc ggctgcaacg cgccggtcac ccacaccgcg cggccggccg
     6901 cggcgatcgc ggccccggtc gcgaactgat tgacgacggg agcgccgccc gcatttgcgt
     6961 gcagcgacgg cgcgacgtcg aagagcggct tgccggtctg cacccgctgc catccctcgc
     7021 tcgcattccg ccggtacagc accagctggc ggggtgtggc cgcgacggcc cacgccgttt
     7081 cgcgatcgat ggtcgccacg cccacgacgt tgcccagaat gtctccggtc ccacccgcgc
     7141 cgcgcaccga tcccagatcg tgctgccagg tgccgtcggt caaacgcaga aacgtcggcc
     7201 cggaaccgac cgcccacccg taggtccccg cggcgtcggt cgccagcgag atggccgtca
     7261 gatcgacggt ggccaccgcg ccactttgcg gatgcacctg ccagcggccg ccggccggcc
     7321 gatgaaagat caggccggtg ccgccgaccc cgtagccctc gcctccttca accatgtcga
     7381 aggccgcgac acccggactg gtcgggtttc cgttcggatc caccagcggc tgttcgatcg
     7441 accatccgcg gccgcgacga taagaaagaa agactgcttg cccttgcggg tcggtctgcg
     7501 accatccggg attgcgccag tgcgtgtgcc cgtaggccca cgcctcggcg tccgccgatc
     7561 ccggcaccgc gacaacgcga gccacacgcg ccggtgcaag caccggatcc atgcgtggtc
     7621 cgtgcagccg gccgaggtcg cggccgacgc tgctgaccgg ccgcggcgaa cgcgtgccgg
     7681 caattccggc gagcgcaccg ctcgtgatcg cgacgatcac ggcgagcgtc gtcgcaagtc
     7741 gggagccgcg gggccggaga aaggcttggc ctcccccggc cccgcgggtg cgttgcgatt
     7801 gcattccggc tcctgcttcg accggcctta ggcctggtcc gtgatcgtcg cgacgccggc
     7861 ctgggcgacc gcgatgaacg atgcgggcat gcgcccgtac ccgacggcgt tcagcttgtt
     7921 ctgcgtgtcg gcgtccaacg cgaccaatag gtagtcggcc aaggtgcggg cctttgtcgc
     7981 gatgaaagcc gatccgaacg cgctcttgag gttgttgtag acgagcgcgt aggtgaaggt
     8041 gcagaccgga tatcccagcg ggttgggcga atcggtgatc gacacggcgt cccagccggg
     8101 ggacaaggtc gacggcggat gcgtcgcacc ggtcgccgac gcttcgcagt tcgcgccgtt
     8161 gccctggtcg ggagcaagga actggcggtg aggaccatcg accagaccaa acgtgaggcc
     8221 cttgctcttg gcggtggaca gctcgacgta gccgatcgcg ccggcgttgt tcttcacctg
     8281 atcggcgaca ccgccgtttc cgtttccgcg cagcggggtg ttcagaccca ggtcctgggc
     8341 cggccacgcg gtgttgagct gattctgctt gtaaacctgg aactgcgggt tgcgcttgga
     8401 caggaagtcc ttgaacgcgt acgtggtgcc cgagccgtcc tgccggaccg cgatcttgat
     8461 cggcttgttg cacgccgcat tcgcaaggcc gggattgtcc accgccagca gcggatcgtt
     8521 ccaccgggtg atgacgcccg agaagatcga gcccacaact tcgctgcgga acttcagcgc
     8581 cttctggccg aaggtgcagc ccgggaagct gacgttgtag gacacggtga tcgagccgag
     8641 cgccagcgga atgtgatgga tcgggctgac gcggttgcgg cccggcgaca gcctgcaggt
     8701 gccgttggcc ggcgccgtga agcccaggtc cagcgtggcg cacagggttt cgtcggtgga
     8761 cagcggctcg tccgacatgc cgaaccagtg tgtgtgctcg atcgcggcga acttgcccgc
     8821 acccgagccg gtcgaggtgt agctgacgtt gcccgacccc gaaccgcagt cggcgttgta
     8881 ttcggggatg aacacgttct ggtgcgcatt gttggcgaac gtagcgccgg tgccctggat
     8941 cgggctcgac accgagcaga atctgccgtt cccgaccagc ggcgcgccca gtgggctgtc
     9001 cggggtctcg gcggcggcga ggccgccgaa cgccatcggc aaagccgtgg cggcggcgat
     9061 cagcagacga acgatcgact ttctcatgat ctgggttcct cccccatgtg ttccggcaga
     9121 cgcatcggaa acgacggtag aaggacgatg tggacggaat gtggcgcccg aacgaacacc
     9181 gc
//
LOCUS       DATLFO010000167        11434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_13145, whole genome shotgun sequence.
ACCESSION   DATLFO010000167 DATLFO010000000
VERSION     DATLFO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13145"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VM841_06590"
     CDS             complement(<1..206)
                     /locus_tag="VM841_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89886.1"
                     /translation="MGEKGLSGAAGDEEYRHAGEPAAGETDAPDAGSDERVWQPDYEP
                     VPAYASDDAPHADQAAASDAGPPAG"
     gene            424..2451
                     /locus_tag="VM841_06595"
     CDS             424..2451
                     /locus_tag="VM841_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89887.1"
                     /translation="MQRSAASFRFERRLARRSARPLRAPAEIVGVEMEFEVVEAGRRL
                     DFRDLIDEVVGGGDRRWFPMDPHARYLPSGAIVTCDGMEAEIATPPVRARRGHPSAVA
                     DEMLTRRAELDTLVRRYNERTGRRIELRGYSTHLNAFLPEGRAPDVAVRFARTVAPAM
                     MLLLDLASSPGALVRPRGRRLELGGEYVAGRDDLRAALTYFIAATMAVERGLTLPLLD
                     GELAEDPSRGGWFVARTAFGEDLYVEGRMASLRLAGGGRILAGTLLGETWEIARPLIE
                     QHATEEELAMVDDTVAGARALPLEREPGPESELAPASAPVQPFARILDERRSNDIGVK
                     PETVSWEAATLKVTTPARSFYARVPLESYAAFATLFERGALDKALRSYGARPPRGRIA
                     ALSTPGIGLYDAIEGPAPATAGGKAPRITIGDAGEPGPAGGMLVTVAKRAAVLAGAAA
                     MFAGTAAFAYLGLRQAGGTAAQPKPRPETSVLAGRESREPVPTEQSTMPTPTPTTAPP
                     SGIITLAPTTPPPVVVPPPTKKASSTPTPRRTTSQPQPSPTPTTSKPKPSSTPTPTPT
                     PTQSQGFQPPAPPPPQSSPSKMTITGRMACNSGTSYLSFSVAQREGGTKIVAMDIQYN
                     SQHLASSPAPSYPYSTYSGTVSSPTGGNKRWYVVVQDQSGAVETQRFDDIC"
     gene            complement(2448..2840)
                     /locus_tag="VM841_06600"
     CDS             complement(2448..2840)
                     /locus_tag="VM841_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89888.1"
                     /translation="MADRRRPSRAAEDWSKPVALGGGWRMQYHKETGEDFTHFRLIPP
                     PNLLPGDAEPVAPRISALDALRVVPWLQRIFKGPEGSRIFRPVAAGEPGALEKAQRAV
                     RAVDKQASLAIDRVPPLPPPRGGRGRSA"
     gene            complement(2917..3558)
                     /locus_tag="VM841_06605"
     CDS             complement(2917..3558)
                     /locus_tag="VM841_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89889.1"
                     /translation="MSYVKLPALEEKGFAVLRSWDGLVPPDEYLGMEYMDWKSGGDTN
                     FAPIASAFGQMECRGFWDHGKPDKDGVWTENALTCPTLVRWTEQVGARFGRVRVIKLE
                     PNTYEQAVHNLHPDDNNRLNPEGDGWVVRAWLQLTDNPDSFMIVREDIDDPSTESRIP
                     LPAGAQFVVDSERLYHAVYHPGPEPRYALIVSFESGPQLEKWMESQRVPQPVA"
     gene            3702..4226
                     /locus_tag="VM841_06610"
     CDS             3702..4226
                     /locus_tag="VM841_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015628998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HVL89890.1"
                     /translation="MLFFEFEGKRPQIAADAFVAPTATIVGDVTVEAGASVWYGAVIR
                     ADYAPVIIRAGANVQDGSVVHAPPEMPVDIGMGATIAHNCVIHGATIGHEALIANGAI
                     VLDGAFVGARSLIAAGSVVTPGTQIPGSVLAFGTPAKVKGPLEGTGAEFWVKLNPPAY
                     QELARRHAAGIKPC"
     gene            4347..5093
                     /locus_tag="VM841_06615"
     CDS             4347..5093
                     /locus_tag="VM841_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HVL89891.1"
                     /translation="MDEILAELAAEQDDLAAVLRGLGDDQWRLPTPAQGWDVRDQVAH
                     LADTEEIAHDTATGGPRQLNEEALTFTSPEEFTESGCIKGRAMEPADVLDWWTAGAAR
                     TRAALASKDPKERIPWGLGMSARAFVTARLMETWAHGLDIRSAVGAQPNITPRLRNVV
                     WLITNALPYALTVAQKPAPEGRTLRVELTGVDGETWTAGPANATDLITGDAAEYCILG
                     VQRMKRADVATLKADGPLAELALDNLRAFL"
     gene            5096..5830
                     /locus_tag="VM841_06620"
     CDS             5096..5830
                     /locus_tag="VM841_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL89892.1"
                     /translation="MSRKRAVVTGASGGIGAATAIALAEHGFDVILGARRLDRLEPVA
                     KQCGGTALALDVTDPASVDEFVRQTGDAHVLINNAGGAKGLDRIEDAVEERWRWMWET
                     NVLGLMRITKAFLPALIASGDGHIVNIGSTAGLEAYEGGAGYTSVKHGVRAITQTLRL
                     ELLGRPVRVSEIDPGLVETEFSRVRFDGDEDRAATVYAGLTPLTADDIAAIVAFVVTR
                     PGHVNIDQVVVRPRDQATSTRVHRRQ"
     gene            6113..6727
                     /gene="mtnB"
                     /locus_tag="VM841_06625"
     CDS             6113..6727
                     /gene="mtnB"
                     /locus_tag="VM841_06625"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:TIGR03328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HVL89893.1"
                     /translation="MNEQALRETLTTLLRLFYAKDWVSGTGGGICAMAGPGRLLVAPT
                     GVHKEMVEPADLFAIDPATCGVIDAAANAALKPSECSPIFAAIVRKRGAGSVVHSHAL
                     DAVLVADLAPGDVLSIRRFEMLKGLKGVGNADDHAVPVIDNTEREPELTGAVEAALKD
                     PRFAKAHAILVRDHGAYIWGEDVWEAKRHTEVYHFLFEGTWTRR"
     gene            6738..7136
                     /locus_tag="VM841_06630"
     CDS             6738..7136
                     /locus_tag="VM841_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVL89894.1"
                     /translation="MYTCPCCQQQQDMTKVDVIDVEAAVAADALTADDPFKRTYLGHG
                     RQSSVFVFQGHAGPFRKHVHETHDEIGYVLAGSGSVTVGEVTRPVKPGDVWIIPSNTP
                     HGGEFGDECRVLFISSPIDDPDNPDRVWLD"
     gene            7177..7608
                     /locus_tag="VM841_06635"
     CDS             7177..7608
                     /locus_tag="VM841_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVL89895.1"
                     /translation="MTKRADPTAALAAVPLFEDLAKKDLRQILDASKEVSFTAGRTIV
                     EEGTTGVAFHLILEGTARVSVKGRTKAKLGPGDYFGEISLIDRGPRSATVSAETDVHT
                     ITLASWNFLSIIDHNPSIARKLLAGLCKRIRETERASAHTH"
     gene            complement(7605..8315)
                     /locus_tag="VM841_06640"
     CDS             complement(7605..8315)
                     /locus_tag="VM841_06640"
                     /inference="COORDINATES: protein motif:HMM:NF025166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HVL89896.1"
                     /translation="MNLPDQRPDPGSLRPVSGGQQDKQGTDEALAAVLGGLAYALLRV
                     FRLSAEATGFAPSLAAAERQAAFAVEEHQRFRALRKRLGAVTDDPEAAVARFRPALDA
                     FYDATPINGWLEAQVFHFVGDTITADFAELFGGVVDERTAAAVREALTGRGAHEGFAL
                     EQIDRARATQADAEDRIVAVASKIVGEAINRVRDAMFASDSLEVVFGGPDAVKDAVLE
                     ILGRHRERLERLGLDRLD"
     gene            8509..9591
                     /locus_tag="VM841_06645"
     CDS             8509..9591
                     /locus_tag="VM841_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL89897.1"
                     /translation="MLLTVVLSTLFAPASHAAESESKKLQQTRTQLKAARSQLSSLKR
                     NEAQLRATMRGVQRNLNSAKNLLSQAQRKLAEIDARIRSHERQIEKLSSLRAERDEAV
                     DKRIASLYMMGPGLQADALMTAGDFKSFADRSNALDFVISRDKRHMEDLAKLRDRERK
                     AQVALRKEAEQAQTWRRRVSERVSLVWDALSTQKQAKAALDAQIAEQMKEVRALEAEQ
                     NRIQSMIARRGSISRGAVSMKGFQWPAKGRRITSSYGRRGGGYHTGMDIDCETGEGTY
                     AAKEGRVIASERAGGYGNMIIIDHGNGVSSLYAHHSRLYVREGAQVERGQKIGACGST
                     GNSTGSHLHFEIRVNGQHRNPRPYLP"
     gene            complement(9588..10466)
                     /locus_tag="VM841_06650"
     CDS             complement(9588..10466)
                     /locus_tag="VM841_06650"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL89898.1"
                     /translation="MPTFQHGSLTLNYEIFGGPEDRTVVFLHGLLLSTAMMEPLARAF
                     SERFRVVNFELHGHGKSSKPTDPGSYTMSAFADDVVALADHLGCDKIGIFGTSLGADV
                     GLEAMLNAPRRVAGAVLEMPVLDTGARTAIRVFARMAKALRSKRAPRLMKTVANRMPS
                     WPPGFPEAAEMLAADPVAGAAVIEGLLREAERDRWAAVRTCSVPALVIGHRLDPLHAY
                     RDAVHLASRLPNGYLLKARSILEMRLRPGRILQRTMDFMDGVFEGLSSPLDVLDEEPG
                     EAAANEAGEAAGEVAT"
     gene            10534..>11434
                     /locus_tag="VM841_06655"
     CDS             10534..>11434
                     /locus_tag="VM841_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016932166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVL89899.1"
                     /translation="MTAGASQPRPTTLGALRESGWRSRSVKDEIRDNLIAAMREGRDS
                     FPGIRGYEDTVIPALERALLAGHDMILLGERGQAKTRLIRSMVALLDEHAPVIDECEI
                     NDDPLAPVCAICRDTVAERGDDTPVAWIPRAERYAEKLATPDIAVADLIGDIDPIRVA
                     EGRYLADERTIHYGLVPRTNRGIFAINELPDLPERIQVGLLNILEERDVQIRGYSLRL
                     DLDVLLVATANPEDYTNRGRIITPLKDRFGSEIRTHYPQHAADEVAIVEQEMHPHAAV
                     PVKIPPFIREILVEFTRLARRCAG"
BASE COUNT         1864 a         4053 c         3864 g         1653 t
ORIGIN      
        1 ccggccggtg ggcctgcgtc ggaggcggct gcctggtccg cgtgcggggc gtcgtcggag
       61 gcgtaggcgg gcacgggctc gtagtccggc tgccacacgc gctcgtcgct ccccgcgtcg
      121 ggggcgtcgg tttcgccggc cgccggctcg ccggcgtgcc gatactcctc gtctccggcg
      181 gcgccggaaa ggcctttctc acccatgggt tcctcctcgc cgaggtctcg ggcttcgtgc
      241 cgcactcttt ctgtgagcgc gcccggcgtc cctgcgaggc gaattgcgct tcgttcggat
      301 cacgtcgttt cgtggcatgc ggatgccgcg aacgattcgg ggaatgcggc cgcctccgac
      361 gcaggcccac cggccggccc gtatgccgac cacaccgccg ccgcgggagg atagcccgcc
      421 gtcatgcaac gcagcgctgc ctcttttcgc ttcgagcgac ggctcgcgcg ccgcagcgcg
      481 cggccgctac gcgcgcccgc cgagatcgtc ggcgtcgaga tggagttcga ggtcgtcgaa
      541 gccggacgcc ggctcgactt ccgcgatctg atcgatgagg tcgtcggcgg cggggaccgc
      601 cgctggttcc cgatggatcc gcacgcgcgt tatctgccgt cgggggcgat cgtcacatgc
      661 gacggcatgg aagccgagat cgccacgcct ccggttcgcg cccggcgcgg ccaccccagc
      721 gcggtcgccg acgagatgct cacgcgccgc gccgagctcg acacgctcgt gcgccgctac
      781 aacgagcgca ccggacgccg gatcgagctg cgcggctact ccacgcacct gaatgccttc
      841 ctgcccgaag gacgcgcccc cgatgtcgcg gtccgcttcg cgcgcacggt cgcacccgcg
      901 atgatgctgt tgctggatct ggcgtcctcg cccggcgcgc tggtgcgtcc gcgcggccgc
      961 cgcctggaac tcggcggcga gtacgtcgcg ggccgcgacg acctgcgcgc cgcgctcacg
     1021 tacttcatcg ccgcgacgat ggccgtcgaa cgcgggctga ccctgccgct gctcgacggc
     1081 gagctcgccg aagacccgtc gcgcggcgga tggttcgtcg cgcgcaccgc gttcggcgag
     1141 gacctctacg tcgagggccg catggcgtcg ttgcggctcg ccggcggcgg gcgaatcctg
     1201 gcgggaacgc ttctgggcga gacgtgggag atcgcgcgtc cgctgatcga gcagcacgcc
     1261 accgaagaag agctcgcgat ggtcgacgac acggtcgccg gcgcacgcgc gctgccgctc
     1321 gagcgggagc ccggcccgga atccgagctc gcccccgcga gcgcgccggt tcagccgttc
     1381 gcgcgcatcc tcgacgagcg gcgcagcaac gacatcgggg tcaagcccga gaccgtgtcg
     1441 tgggaagccg cgacgctcaa ggtgaccacg ccggcacgct cgttctacgc gcgcgtgccg
     1501 ctggaaagct acgccgcgtt cgccacgttg ttcgaacgcg gcgcgttgga caaggcgctg
     1561 cggtcgtacg gcgctcgccc gccgcgcggc cgcatcgccg ccctctccac gcccggcatc
     1621 ggcctctacg acgcgatcga ggggccggcg cccgcgaccg cgggcggcaa ggctccccgg
     1681 atcacgatcg gcgacgcggg cgagccgggt cccgcgggcg gcatgctcgt caccgtcgcc
     1741 aagcgcgcgg ccgtgctggc cggggccgcg gccatgttcg ccggcaccgc cgcgttcgcc
     1801 tacctcgggc ttcggcaagc gggcggaacg gcggcgcagc cgaagccgcg gcccgagacc
     1861 agcgtgctgg ccgggcggga gagccgcgag cccgtcccca ccgagcaaag cacgatgccg
     1921 acgcccaccc ccacgaccgc gccgccctcg ggcatcatca cgcttgcacc gacgacgccg
     1981 ccgccggtcg tcgtcccgcc gccgacgaag aaggcctcgt cgacgcccac gccgcgccgt
     2041 accacctcgc agccccagcc ctcgccgacc cccaccacgt ccaagccgaa gccgagctcg
     2101 acgcccacac ccacgccgac gccgacgcag tcgcaaggct tccagccgcc cgcgccgccg
     2161 ccgccgcagt cgagcccctc gaagatgacg atcacgggcc gcatggcgtg caacagcggc
     2221 accagctatc tcagcttcag cgtcgcgcag cgcgaggggg gcacgaagat cgtcgcgatg
     2281 gacatccaat acaacagcca gcatctggct tcgtcgccgg cgccgtcgta tccctactcg
     2341 acctactcgg gcaccgtgtc gtcaccgacg ggcggcaaca agcggtggta cgtggtcgtg
     2401 caggatcagt cgggcgcggt cgagacgcag cgcttcgacg atatctgcta ggcggagcgg
     2461 ccccggcccc cgcgcggcgg cggaagcggc ggcacccggt cgatcgcgag cgacgcctgc
     2521 ttgtccacgg cgcgaaccgc gcgctgggcc ttctcgagcg cgcccggctc gcctgcggcg
     2581 accggccgga agatgcgcga gccctcgggc cctttgaaga tgcgctggag ccacggcacg
     2641 acgcggagcg cgtccaaggc ggagatgcgg ggcgcgaccg gctcggcgtc gccgggcagc
     2701 agattcggcg gggggatcag gcgaaaatgc gtgaagtcct cacccgtctc cttgtggtac
     2761 tgcatgcgcc agccaccgcc gagagcgacc ggcttcgacc agtcctcggc ggcacgcgac
     2821 ggtctgcgtc gatcggccat agggcgctaa gggtacccga tagaaaaaga atcggagcgg
     2881 cgatcgctcg ccgctccgag gttcggtgaa ccgctgctac gcgaccggct gcgggacgcg
     2941 ctgcgactcc atccacttct cgagctgcgg gcccgattcg aacgacacga tcagcgcata
     3001 gcgtggctcg ggacccgggt gatagacggc gtggtagagc cgctccgagt ccaccacgaa
     3061 ctgggcaccc gcgggaagcg gaatgcggct ctcggtggac gggtcgtcga tgtcctcgcg
     3121 cacgatcatg aacgagtcgg ggttgtcggt gagctgcagc catgcgcgca cgacccagcc
     3181 gtcgccctcg gggttgagcc ggttgttgtc gtcggggtga aggttgtgaa ccgcctgctc
     3241 gtaggtgttg ggctccagct tgatgacacg cacgcggccg aagcgagcac cgacctgctc
     3301 ggtccagcga accagcgtcg ggcacgtcag cgcgttctcg gtccagaccc cgtccttgtc
     3361 cggcttcccg tgatcccaga agccgcggca ctccatctgg ccgaacgccg acgcgatcgg
     3421 agcgaagttg gtgtcgccgc cggacttcca gtccatgtac tccatgccga ggtactcgtc
     3481 cggggggacg agaccgtccc agctacggag cacggcgaag cccttttctt ccagggcggg
     3541 aagcttgaca taagacatat atgttcctcc tggggaaacg ttcgcgcgca tgatacccga
     3601 gagcgagtag gaaggttggg attcccagaa gtgggtatag cctagcaacc attttccgga
     3661 gggatggtca cgctgcggcg tgggatgggg gcccgataac aatgctattc ttcgaattcg
     3721 agggcaaacg gccgcaaatc gccgccgacg cgttcgtcgc gccgaccgcg accatcgtcg
     3781 gtgacgtcac cgtcgaggcc ggagcatccg tttggtacgg cgccgtcatc cgggccgact
     3841 acgcgcccgt gatcatccgg gcgggcgcca acgttcagga cggcagcgtc gtccatgcgc
     3901 cccccgagat gcccgtcgac atcggcatgg gcgcgaccat cgcgcacaac tgcgtgatcc
     3961 acggcgcgac catcggccac gaggccctga tcgccaacgg agccatcgtg ctggacggcg
     4021 cgttcgtcgg ggctcgatcc ctcatcgcgg ccggctcggt cgtcacaccg gggacgcaga
     4081 tcccgggcag cgtgctcgcc ttcggcactc cggccaaggt caagggtccg ctggagggca
     4141 ccggcgccga gttctgggtc aagctcaacc cgcccgccta ccaggagctc gcgcgccggc
     4201 acgcggccgg catcaagccc tgctgatctc gcttgaatag ccttttcgtc agggctacat
     4261 agccctatgg aaagggccac ggtgacggac gcgaagaatc gtctcagtgc ctgtcttcgt
     4321 aacgagcgct tgatagcctg cgcggcatgg atgagatcct tgccgagctt gcggcggaac
     4381 aggacgacct tgccgcggtg cttcgcggtc tgggcgacga tcagtggcgg ctgcccacgc
     4441 ccgcgcaggg gtgggacgtg cgcgatcagg tcgcgcatct ggccgacacc gaggagatcg
     4501 cacacgacac cgcgaccggc ggaccgcgcc agctcaacga agaagcattg accttcacct
     4561 cgcccgagga attcaccgag tcgggttgca tcaagggccg cgcgatggag ccggccgacg
     4621 tgctggactg gtggacggcc ggcgcggcca ggacgcgcgc ggcgctggcg tccaaagatc
     4681 cgaaagagcg catcccctgg ggcctgggca tgagcgcgcg cgcattcgtc accgcgcggc
     4741 tgatggagac ctgggcgcac ggcctggaca tccgctccgc cgtcggcgcc cagcccaaca
     4801 tcacgccgcg gctgcgcaac gtcgtgtggc tgatcaccaa tgcgctcccc tacgcgctga
     4861 ccgtcgcgca aaaacccgcg cccgaaggcc gcacgctgcg cgtcgagctg accggcgtgg
     4921 acggcgaaac gtggacggcc ggcccggcga atgcgaccga tctgataacc ggcgacgcgg
     4981 ccgaatactg catcctgggc gtgcaacgca tgaaacgcgc cgacgtggcg acgctgaaag
     5041 ccgacgggcc gctggccgag ctggcgctcg acaacctgcg cgcattcctc tagccatgag
     5101 ccgcaaacgc gcggtcgtca ccggcgcgtc cggcggcatc ggcgcggcca ccgcgatcgc
     5161 gctcgccgag cacggcttcg acgtcatcct gggcgcgcgg cgcctcgacc ggctcgaacc
     5221 ggtcgcgaag caatgcggcg gcaccgcgct cgcgctcgac gtcaccgatc cggcaagcgt
     5281 ggacgagttc gtccggcaga ccggggacgc gcacgtgctg atcaacaatg ccggcggcgc
     5341 aaaagggctc gaccgcatcg aagacgccgt cgaggaacgc tggcgctgga tgtgggaaac
     5401 caacgtcctc ggcctgatgc gcatcaccaa agcgttcctg cccgcgctga tcgcgagcgg
     5461 cgacggccac atcgtcaaca tcggatcgac cgccggcctg gaagcctacg aaggcggcgc
     5521 cggctacacc agcgtcaaac acggcgtgcg cgccatcacc caaaccctgc gcctcgagct
     5581 gctcggccga cccgtgcgcg tcagcgaaat cgaccccggc ctggtcgaaa ccgaattcag
     5641 ccgcgtccgc ttcgacggcg acgaggaccg ggccgccacg gtttacgccg gcctcactcc
     5701 gctgaccgcc gacgacattg ccgccatcgt cgccttcgtc gtcacccgcc ccggccacgt
     5761 caacatcgac caggtcgtgg tccgaccgcg cgatcaagcc acttccacgc gggttcatcg
     5821 acggcaatga tcgaccctcg gatccctggg agtcacgcgg tggacaccac catcgggttg
     5881 gctcccttgt gctcggcccc accgaatggc gtagtcaagg cccatttccg agacaacacc
     5941 ttcaacggaa tctttgtgaa agagatgctc gttctgcgcg ccgcctgaca gctgagcggg
     6001 ccggacggtt cgaatctcat ccggcgtggg actgcccgaa actctgtgtc cgtgcgttat
     6061 cgcgaagcct gttcgacgga gccgggtcgc ggccaatagc atctcggccg atatgaacga
     6121 acaggcgctt cgcgagaccc tcaccacgct gcttcggctt ttctatgcga aagactgggt
     6181 gagcggcacg ggcggcggga tctgcgcgat ggccggcccg ggccggctgc tggtcgctcc
     6241 caccggtgtg cacaaggaga tggtcgagcc ggccgatctg ttcgcgatcg atcccgcgac
     6301 gtgcggggtg atcgacgcgg cggcgaacgc ggcactcaag cccagcgaat gcagcccgat
     6361 cttcgcggcg atcgttcgca agcgtggcgc tggctcggtc gtgcattcgc acgcgctcga
     6421 tgccgtcctg gtcgccgatc tcgcgcccgg cgacgtgctg tcgatccgcc gcttcgagat
     6481 gctcaagggc ctcaagggcg tcggcaacgc cgacgaccac gcggtcccgg tgatcgacaa
     6541 caccgaacgc gagcccgagc tgaccggcgc ggtcgaggcc gcgctgaagg atccgcgctt
     6601 cgccaaggcg cacgcgatcc tggtgcgcga ccacggcgcc tacatctggg gcgaggacgt
     6661 ctgggaggcg aagcgccaca ccgaggtgta tcacttcttg ttcgaaggaa cctggacacg
     6721 ccgataaggg agatcgcatg tacacgtgcc cgtgctgtca gcagcagcaa gacatgacca
     6781 aggtggatgt gatcgacgtc gaagccgccg tcgctgccga cgcgctgacc gccgacgatc
     6841 cgttcaagcg cacctatctg gggcacggcc gccagtccag cgtcttcgtc ttccagggcc
     6901 acgcgggccc gttccgcaag cacgtccacg aaacccacga cgagatcggc tacgtcctgg
     6961 ccggcagcgg cagcgtgacc gtcggagagg tgacccggcc ggtcaagccc ggggacgtgt
     7021 ggatcatccc gtccaacacc ccccacggcg gcgagttcgg cgacgagtgc cgggtgctgt
     7081 tcatctcgag cccgatcgac gaccccgaca accccgatcg ggtctggctg gactgagtat
     7141 cattcggccc acaagacttc cgaaaggacc tgccagatga cgaagcgtgc cgacccgacc
     7201 gccgccttgg cggccgtccc gctgttcgaa gatctcgcca agaaggacct gcggcagatc
     7261 ctcgacgcgt cgaaagaggt gtcgttcact gccggccgca ccatcgtcga agagggcacg
     7321 accggcgtgg cattccattt gatcctggag ggcaccgcgc gcgtgtcggt caagggccgg
     7381 accaaggcca agctcggtcc gggcgattac ttcggcgaaa tctcgctgat cgaccgggga
     7441 ccgcgctcgg cgaccgtctc ggccgaaacc gacgtgcaca ccatcacgct cgcctcgtgg
     7501 aacttcctgt cgatcatcga ccacaacccg tcaatcgcgc gcaagctgct cgccgggttg
     7561 tgcaagcgga tccgcgagac cgagcgcgcc tcggcgcaca cccactagtc gagccggtcc
     7621 agccccaacc gctcgagccg ctcccggtgc cggccgagga tctcgagcac cgcgtccttg
     7681 accgcgtcgg gtccgccgaa gacgacctcg agcgagtcgg acgcgaacat cgcgtcccgc
     7741 acgcggttga tggcctcgcc gacgatcttg cttgccacgg ccacgatgcg atcctcggca
     7801 tcggcttggg tggcgcgggc gcggtcgatc tgctcgagcg cgaacccttc gtgggcgccc
     7861 cggccggtca gtgcctcgcg caccgccgcc gcggtccgct cgtcgaccac gccgccgaac
     7921 agctcggcga agtcggccgt gatggtgtcg ccgacgaagt ggaagacctg cgcctcgagc
     7981 cagccgttga tcggggtggc gtcgtagaag gcgtccagcg ccggccggaa ccgggcgacc
     8041 gccgcctcgg ggtcgtcggt cacggcaccg aggcgctttc gcagcgcacg gaagcgctga
     8101 tgctcctcga cggcgaaggc cgcctggcgc tcggcggcgg ccagcgaagg cgcgaacccg
     8161 gtcgcctcgg ccgacaggcg gaagacccgc aagagggcat aggcgagccc tccgagcacg
     8221 gcggccaggg cctcgtccgt gccttgcttg tcttgctgac cgcccgaaac cggccgaaga
     8281 gagccggggt cgggacgctg atcgggcagg ttcacgtggg cattttcgca ggtcgcagcc
     8341 ccgggtggta gggcgtcttt gaatctcgtc cgcgactttg gtacacactg gcgggcccta
     8401 ggagccggaa atcagatgtt tccgccgcac ccgggggcac cgaggagccg cgttttgcgg
     8461 ggagggagct ggagcttgat tcgcgcacgt cgccgttgca tttcgatagt gctcctgacc
     8521 gtcgtcctgt ccaccctctt cgcaccggcg agccacgccg ccgagtccga gtccaaaaag
     8581 ctccagcaaa cccgcaccca gctcaaggct gcacgcagcc agctttcctc gctcaagcgc
     8641 aacgaggccc agcttcgtgc gaccatgcgc ggcgtgcagc gcaacctgaa ctcggcgaag
     8701 aaccttctgt cgcaggctca gcgcaagctg gccgagatcg acgcgcgcat ccgctcgcac
     8761 gagcggcaga tcgagaagct ctcgagcctt cgggccgagc gtgacgaggc cgtggacaag
     8821 cggatcgcct cgctctacat gatgggaccc ggcttgcagg ccgacgcgct catgacggcg
     8881 ggcgacttca agtcgttcgc ggaccgctcc aatgcgctgg acttcgtgat cagccgcgac
     8941 aagcggcaca tggaggacct ggcgaagctg cgcgaccgcg agcgcaaggc gcaggtcgcg
     9001 ttgcgcaagg aggccgagca ggcccagacg tggcgccgca gggtcagcga gcgcgtctcg
     9061 ctggtgtggg acgcgctgtc cacgcagaag caggcgaagg ccgcgctcga cgcgcaaatc
     9121 gccgagcaga tgaaggaagt gcgcgcgctc gaggccgagc agaaccgcat ccagtcgatg
     9181 atcgcccgtc gcggctcgat ctctcggggc gcggtgagca tgaagggctt ccagtggccg
     9241 gcgaagggcc gacgcatcac cagctcgtac ggccgtcgcg ggggcggcta tcacacgggc
     9301 atggacatcg actgcgaaac cggtgaggga acctatgcgg cgaaggaagg ccgcgtcatc
     9361 gcctccgagc gtgccggcgg atacggcaac atgatcatca tcgaccacgg caacggcgtt
     9421 tccagcctgt acgcgcatca ctcgcggctc tacgtccgcg aaggcgccca ggtggagcgc
     9481 gggcagaaga tcggcgcctg cggctcgaca ggaaactcga ccggcagcca cctgcacttc
     9541 gaaatccggg tcaacggcca gcaccgtaac ccgcggccgt atctgcccta agtcgcgacc
     9601 tcgccggccg cttcgcccgc ctcgttggcg gctgcttcgc ccggctcctc gtcaagcacg
     9661 tcgagcggcg agctgagtcc ctcgaacacc ccgtccatga agtccatcgt ccgctgcagg
     9721 atccggccgg gccgcagccg catctcgagg atgcttcggg ccttcagcag atagccgttg
     9781 gggagccgcg acgcgaggtg cacggcgtcc ctgtaggcgt gcagcgggtc cagacggtgg
     9841 ccgatcacca gcgcgggaac cgagcaggtt cgcacggccg cccaccggtc acgttcggcc
     9901 tcccgcagca ggccttcgat caccgcggcc cccgcgaccg gatcggccgc gagcatctcc
     9961 gccgcctcgg ggaagcccgg cggccacgac ggcatgcgat tggcgacggt cttcatcagg
    10021 cgtggagccc gcttgctccg caaggccttc gccatccggg cgaacacccg gatcgcggtg
    10081 cgggcaccgg tgtcgaggac cggcatctcg agcacggccc cggccacgcg ccgcggcgcg
    10141 ttgagcatgg cctcgagccc gacgtcggca cccagcgagg tgccgaagat cccgatcttg
    10201 tcgcatccga ggtgatcagc gagcgcaacg acgtcgtcgg cgaaggcgct catggtgtag
    10261 ctgcccgggt cggtcggctt ggaggacttg ccgtgcccgt gcagctcgaa gttcacgacc
    10321 cggaatcgct cggagaacgc gcgggcgagt ggctccatca tcgcggtgga cagcagcaga
    10381 ccgtgcagaa agacgacggt ccggtcttcc ggcccgccga agatttcgta gttgagcgtg
    10441 agcgatccgt gctgaaacgt cggcattggc gtcgatgcgc ggccgccgga cgagccccgg
    10501 cgtccccgcc gcgcgagcgt tagcataggc ggcatgaccg ccggggcaag ccaaccgcgt
    10561 cccaccacgc tcggcgcgct gcgcgagtcc gggtggcgct cgcgcagcgt caaggacgag
    10621 atccgcgaca atctcatcgc cgccatgcgc gaaggccgcg actcgtttcc cgggattcgc
    10681 ggctacgaag acacggtgat cccggctctc gagcgagcgc tgctggcggg ccacgacatg
    10741 atcctgctgg gcgagcgagg ccaggccaag acgcggctga tccgctcgat ggtcgcgctg
    10801 ctggacgagc acgcgccggt tatcgacgaa tgcgagatca acgacgatcc cctggctccg
    10861 gtctgcgcga tctgccgcga caccgtcgcc gagcgcggtg acgacacgcc ggtcgcgtgg
    10921 attccgcgcg ccgagcgcta cgccgagaag ctcgcgacgc ccgacatcgc cgtcgccgac
    10981 ctgatcggcg acatcgaccc gatccgggtc gcggaagggc gctacctcgc cgacgagcgc
    11041 acgatccatt acgggttggt gccgcgcacc aaccgcggga tcttcgcgat caacgagctg
    11101 ccggacctgc ccgagcgcat ccaggtcggc ctgctcaaca tcctcgagga gcgcgacgtg
    11161 cagatccgcg gctactcgct gcgcctcgat ctcgacgtcc tgctcgtcgc caccgccaac
    11221 cccgaggact acaccaaccg cggccgcatc atcacgccgc tcaaggatcg cttcggcagc
    11281 gagatccgca cgcactatcc gcagcacgcc gccgacgagg tcgccatcgt cgagcaggag
    11341 atgcacccgc acgcggcggt gccggtcaag atcccgccgt tcattcgcga gatcctggtc
    11401 gagttcaccc ggctcgcgcg ccgctgcgct ggtt
//
LOCUS       DATLFO010000168        33683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_93467, whole genome shotgun sequence.
ACCESSION   DATLFO010000168 DATLFO010000000
VERSION     DATLFO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 33683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33683
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93467"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VM841_06660"
     CDS             <1..1419
                     /locus_tag="VM841_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVL89900.1"
                     /translation="VQGLAVGAASVVAVTVAASLTLLPALLGFAGQKIELTRWRGLIA
                     AGFVAIALAGVGLDITPLLVGLPVAAVVLIAGLFAAPLKREVRHRSRKPLRETAAYRW
                     SRVVQHHPWKVALISSGVLIALALPFFSLRLAFSDEGNYGEETSTRKAYDLLAEGFGP
                     GFNGPMIMAAEIPRGTDRAALQAVTDAAQRDPGVAFVSPPVISEDFSAVLWRLIPTTA
                     PQDEATAATVRRLRTQVLPPAASRLGMDVTITGAVAVSQDFGNYLGSRYPYFFGAVLI
                     LSFLLLMAVFRSVLVPVKAVIMNLLSIAAAYGVVVALFQWGWLSAITGVQPAPIEPWA
                     PMMLFAIVFGLSMDYEVFLLSRVREEWRRTGDSRRSVADGLAATAKVISAAAAIMVVV
                     FGSFILEQDRVIKLMGTGLAMAILLDATIVRMLLVPATMELLGDKNWWLPRWLDKILP
                     RVDVEGTATPVDQIDEPVTGVR"
     gene            complement(1386..2324)
                     /locus_tag="VM841_06665"
     CDS             complement(1386..2324)
                     /locus_tag="VM841_06665"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HVL89901.1"
                     /translation="MSTETVDAALARLAARQFGVFSRGQALPLGATARMIQRRCDAGR
                     WLRVGHGIYAIAGHPLSWNSEMFMALLSAGAGSAASHRAAARLWRFPDCSDAAVEITH
                     PTYRGRRDFIAHRSALHRRDVTHIGEIPVTSPARTLIDLAAVVPDATVGAALDDALRR
                     RLITIPRLMRRADALAQRGRRGIEVVRAAAADRDGLPALLQSPLESAVYDLIVTSGLP
                     RPIPQCEILLGARRAFIDFAYPDAMFGIEADGYRWHAGRDVWQSDLARRNALTSAGWR
                     ILHVTDEDVKRRRDGVVDAIRSGLAAYRTPVTGSSI"
     gene            complement(2416..5160)
                     /locus_tag="VM841_06670"
     CDS             complement(2416..5160)
                     /locus_tag="VM841_06670"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HVL89902.1"
                     /translation="MTKILTSILLALPLAGAYSMFALGIVVIYRASRILNLAHGAMAT
                     LPAYLAYSMSKAGVPMFLVLPLAIASGAVLGMIVERGVLRPLRSESQTTQTVGTVAVL
                     GVLVALIARVWGTAPLAAPTIFPDGSIDVGNSILQYGEMGLALVALIAAGSIAMMLQY
                     TDIGLAMRGAADNRRAASLMGVDPDRTTSAAWALGGALAATAGVLLAGVTNLEPYTLS
                     RQVLPAFVAALLGGLGSLGGALAGAAIVGLVQGVVGGFTASPGVGQLVLTILALGVMA
                     LRGKKISGTEEDSGRGAAIRKPKRLRLRSPWVWVTLVVALAWPWVPFPSSMAGLADSL
                     HGDAALAAIFTIAAVSLVVLTGWVGQISLAQASFVGLAAFSTGLMVKKFGIPFPLNLP
                     IAAAIAGGAAALLGVVALRVRGLYLAVATLIFGWMADAYLFKQAWLVGEGGSTIIGNT
                     VVGREGAFPSFDLTQRRIFYYVALAGAALAIAAAANWRDSRTGRAMFAVRGSELAAAS
                     MGINVTRTKLFAFAVSGVLAGIAGNLIMIDQRTANADQFSFKWSLFYLAIAAVGGLTR
                     LGGAAAAAALFAALNEIFFRLTFLNGYLELVSGGLLLIAILARAEPVRKLLARRSAVE
                     APRAMLDPPQLPAAAAPPSANGQGPPPPALEASGITVRFGGLTAVGEASLRVERGETV
                     GLIGPNGAGKTTMFNAIGGMNSPQAGSVSIAGRVVTALGVHERAALGMGRTFQAIQLF
                     KESTVFDNLMVATHLHGTTGPGSHLLLTDKALKDEEAARARVHAAAATVGLGPVLDAR
                     CGDLPFGVLRMVEIARALVTGANLILLDEPASGLDERETDRLRELLVALREGLGLSLL
                     IVEHDVRFVMSLSDRMYVLDQGRIIATGTPAEIRSDPAVIAAYLGTDAASTPEPALIH
                     "
     gene            complement(5157..6593)
                     /locus_tag="VM841_06675"
     CDS             complement(5157..6593)
                     /locus_tag="VM841_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89903.1"
                     /translation="MKRKIFKALPALLAVVLLSTVSFATRYVSKPETRTYTVGQDGAP
                     TDPSQPGDPSAAPGATANPGDGPGAAPVVRGGKTLECVRGRNGGATDTGVTNSSIKLG
                     ATVVKTGIGASFLKEVPIALQAVVNKVNRGGGVCGRLLNLKMVDDGWDAARGQQFIRN
                     FISEGVFALAVSPSSEGLDAAIRNGDISKAGIPVVGADGMLISQYTDPWVWPVAASTI
                     TTMHVMAKNARDRGAKTFGLVYDKSYHFGVEGASAFRGAIRRMMGSTAAIKADIGIEA
                     GQPSYKNDVDRFNGRCNPCDFVAMLLEPPTALQWIRDGASFGGMQPGGTGGPQPLFVD
                     SFARACGQPCNGLWVWTGYTPPIPPFDDLPAVSAYVNDLRANSSTVDVNNPFVEGGYL
                     GMQLLVKALRQVGPDLTRERLRDVLDSTVLDDGLTQPLRWRRGNHFANNSVQAFSVVM
                     NNGSFEKWRDERTGWIADPWVGMDAPKR"
     gene            complement(6590..9160)
                     /locus_tag="VM841_06680"
     CDS             complement(6590..9160)
                     /locus_tag="VM841_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89904.1"
                     /translation="MQGIGKAANPGAPRLRVRRALLRLVVVAVVAPAVFTALPDAPAT
                     ADGGMRTVGTLDRRTGLDSIAANGNILIEAKARRGWQASLREGRMLLFEVDLDSLKLL
                     STTEIPELMPAVTTSGPTEWQWRLEESTRRIIAIERTVDPQLRLEAHRLIWIDLRTKK
                     VTQGPQLWPGSTRFPLALATHDGGKTVYLLTRAPTEVMGRTRLWLEERALDGTRRFEY
                     LLDDCFNAQDMQYAPVLARSRLRPGTIYVTCYGPGNVQAQVLRVTLTAAGPAGVEVFP
                     GVPGPLSAQFDAGSDRVFFLTTNGGAGRGAWVFDGLRSSFLGVIASGDNRVGASDYAM
                     GIDPATGRLYMETPAGFLIADARRTPLPAGLLFRELAGQVIGSIHVDPVTRRVFLPSP
                     FVKNPQGQSTEYLILKDTIRVSKDPPPGDPDALTADVAERKGVTDVNYTGGARAFAMR
                     ALTTGGAQRMVWNLALGQFSPEDRGFDAAAAAVATVPLDQSNRDLYAARVHNVSIGNA
                     SSEAAASFGHADAGTLRDAERAGRAWPFPAAECRDDAGRPESKNEAGSSASCDLDATS
                     VTGASALTGSATDEGALLSTRALVAFGNVSRNERDGLVARAIAVVQDVSIGNRVRIGE
                     IRAEALTRAKGRPGTAAGSFERFIENVRIDTDGNGTVDFTCELCRPQAVAAAIEQALP
                     GQVKVEFPSPDTAWFGGSKRGYQAVVQKETFRGYAEKTLNDDDAPEVTGMQVTFFADG
                     RAGRSRQVVQLAGVQAESHYGIYLLGQDAPEPEPEPEPSVEPVPTVEPTVDAPVPPPP
                     APVVKGTKTVTETFVRRIAAGSALALAKPKDALLLGALWLFLGLPAYLILRRRHVGAR
                     "
     gene            complement(9187..9999)
                     /locus_tag="VM841_06685"
     CDS             complement(9187..9999)
                     /locus_tag="VM841_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89905.1"
                     /translation="MRHKKVVYKMLAIGLALGSMFAPASSSAHPAGEHVMYIDGTSNI
                     SGYCQYEWKRVGNNPVRDDLGIPQPAQLPPVCRDADPNAPGVQIATCRLQRNLGTYRP
                     YWRAADTTQGEVGLNPPCMGQISANTDLLGGCVDATTGDTTCRINAPTWFYGYCGQTY
                     GGAEGGTLRFQGEDWTINRMGFPRGRGAWEFSGVISRAGHAPSKIRFYFGAAPTKTDE
                     MAACDVGGNLTQITFNGIAIVSTNLPARVIRTRPDWHFCTQDPILGTNGDGC"
     gene            complement(10182..11438)
                     /locus_tag="VM841_06690"
     CDS             complement(10182..11438)
                     /locus_tag="VM841_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HVL89906.1"
                     /translation="MTRSRVAVRIAVATLVAAGSLGNLASLRTPAHATSSRTLEATFT
                     SVSFGWAKVERWKDNSTGFTAEGWPSDGRGNQQGQRVTHFGSSQPHSSRFLLSYAPKW
                     STGTKPVPVLLVHGANQTADFAWANPNEYGGNLCGASTCPSTGLMQYLDSRGYKVFGI
                     TFPHKNGDGYYWAEQIFDAIEIIKAATGASQVDVVAWSKGGFNARQYISSVKRSWGSA
                     YNGNVRRLILLGTPNNGFDYAFRHGINTSVGIYPECGINANGAAPHTWLVCYGVWYNH
                     PEYAYDSSWYPGSKQMLKRWDGTYGLPVAEQDYYTTYYGGWGYVSYSAGITAYTGSSL
                     VDTIRSAGIPSSVRTYLLCGQTADIPNIHNEHTGTSDGLIFKNSCTSSTGIANLGGTV
                     TLSINHSKLGWNSTAMSQVEAWLAAA"
     gene            complement(11489..12322)
                     /locus_tag="VM841_06695"
     CDS             complement(11489..12322)
                     /locus_tag="VM841_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL89907.1"
                     /translation="MTRIDTGTGVALDYEVHGNEGPWVVLIMGLAYGRWGWHWNAGPL
                     AERGYRVVTFDNRGVGGSDAPPGPYTAAEMAADTAGLMDALGIERAHVVGTSLGGFVA
                     QELALARPELIERLVLACTGFGGADFVPMPQRTLDLIELAPTLPDEERLRRFIENAFS
                     DEFVTQRPDVVQTVIDFRRESNQPLESYMAQWNAGATFDASDRVHVIEAETLCITGLE
                     DAVVDPRNTPLLAERVPGAAITTLPGGHLFFIEQAREFNRIVADFLAGGRDAIREVRT
                     A"
     gene            complement(12319..13344)
                     /locus_tag="VM841_06700"
     CDS             complement(12319..13344)
                     /locus_tag="VM841_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HVL89908.1"
                     /translation="MTSVYRNPPVGIADLGHYAPEHFMTAAQIGERAGIPEEIVVERF
                     GLKGKHIAAWDEHVSDMCIAAAEPIVERNGRADIDAVVYFGSHWKDYAVWQAAPAIQH
                     ALGLDGFAMEAVNVSAGAPVALKVVRDMLAADPHLRSVLLVGAAKESQLLDYTNKRAR
                     FMINFGDGGVAVLLRRDHPHNVVLGSSILTDGSFSDQVRVPAGGSAHPASHATVDRRM
                     HYLDVRDPQEMKDRLDPITLKNFLKVASQALERSGYGINDVDLLLPIHMKRSIHHMLL
                     GEFGLSENQSIYLDTFGHMSAVDPLLALSVARDEGGLRDGMTILTLAAGTGYSWAASV
                     IRWGSDA"
     gene            complement(13341..14876)
                     /locus_tag="VM841_06705"
     CDS             complement(13341..14876)
                     /locus_tag="VM841_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL89909.1"
                     /translation="MTFPYTGIGFWIARRAALTPNRIALVTPDGSYTYRELDERAARA
                     AGALAAAGVEVGDRVATYLPNTVDYVDAFFACARLGAIMVPLSTRLTAPELEFLCNDS
                     QSATLLYDASLREAVAAFRPGTAIKAAFVAGSGDAADPSDLRWDDAQSAATPAGSPAG
                     AKPEDALAIIYTSGTTGRQKGAVLTHGNFFWTNLNMIVALDITQDERSLVVLPMFHIG
                     GWNVNTLAVWWKGGTVYLEPAFDAGRVLDLIAREKITSMMGVPTIYQVLADHPRFAAS
                     DLSSVRSFVCGGAPLAVPLIRRYQERGLQFIQGYGLTEAAPNCLILAPEDAIRKAGAA
                     GRPYFFADTRVFDEHDNEVAPGGTGEIVVGGPSVMREYWNLPEETAKALRGGWLRTGD
                     VGLIDDEGYITIVDRVKDMYISGGENVYPAEIERTLAGHPAVQEAAVIGVPDERWGES
                     GRAIVVLRTDASATEDEIRAFCSERLAKFKVPASVVFAETLPRNATGKLLKPELRARY
                     GSP"
     gene            complement(14873..15562)
                     /locus_tag="VM841_06710"
     CDS             complement(14873..15562)
                     /locus_tag="VM841_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89910.1"
                     /translation="MKRRIALLCAAALLAALPARADHQAGHSSPQGPAKIRFPTDFPA
                     LTDHEWGFRIGGFGGIASGAPRTHAPVIFVHGNNVDHADWYPVRDRFKAAGWTDQELW
                     ALSYNGLGANNGSALFTQNPERDAERAEMGWDGNARVTSNDVNVPDLLDFIAAVRAYT
                     GSERFSIVAHSLGVTLARKALKVRPALRQGLVAFVGIAGANDGTSFCPPGSEGVVVSC
                     DVYRTFLEAAE"
     gene            complement(15559..16635)
                     /locus_tag="VM841_06715"
     CDS             complement(15559..16635)
                     /locus_tag="VM841_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL89911.1"
                     /translation="MRAVVAGLALLLAAASAGADHQNGHTPPVGPMKAHFPAGFPALT
                     DAEWSFRLGGFGGIRQGAPLKHVPVIFVHGNNVDHADWYHVRDDFKAAGWTDQELWGL
                     SYNGVGNDNGNTPSRDNPAGRAERAEMGGDGIARITANDVNVPDLHAFVTAVRAYTGS
                     KKFTIVAHSLGVTVARKMLKVHPALRADLVAFVGIAGGNHGTSLCPPGSEGNVYACDE
                     IAANTPWLAELNGPDGSDETYPPAKWMTVYEGTGTYDVAFMGTYAQSPVLKGADNRQY
                     PFTNHNQLRVAANVVADYRAFIENAEKPYLAAPTTPAPKPAAVLGDKRELAATGVPGA
                     LFGAGALTLGAAAFGALTIRKRTR"
     gene            complement(16632..17513)
                     /locus_tag="VM841_06720"
     CDS             complement(16632..17513)
                     /locus_tag="VM841_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL89912.1"
                     /translation="MRAPELFDVPVAGGTLRVARWPGRDGAPVALCAHGVTATHRAWS
                     LIAEHLDGDVTVVAPDLRGRGGSNAITGPFGIPQHTEDLAAVLDHLSLPNAVFVGHSM
                     GAFVVAVAAVRHPERVGGLVIMDGGIKLLDAPVEADIDALLHQIIGPSMERLKTTFAS
                     RAAYQDFWRRHPAFAGDFNEHVQAYVNYDLIGDEPALRSGVSLEAVYADSRDTLTDPL
                     TVTAIEQITRPAIFLRAQRGVMNEPSGLYSHEQIAALQARVPHLEPHLLEASHFSMML
                     GQGSAEVAAFIRKACGA"
     gene            complement(17514..19124)
                     /locus_tag="VM841_06725"
     CDS             complement(17514..19124)
                     /locus_tag="VM841_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL89913.1"
                     /translation="MTIPGLMQHTPLTLRAIFDRMRDVYADGKVVSPEGRTTYGALSE
                     EVLKLVTVLHGLGVRSGDRVGTFAFNSARHFALYHAVPLIGAVLHTINVRLYDEQIAY
                     IANHAEDRVIFVDAELCGALAEVAPKLATVGAYVVMGDGDAHGLTPAHDYDSLLSAAA
                     PAAPDSLPTIDEHAGCGLCYTSGTTGMPKGVLSSHRGMWLHSMATAMAGTLALTETDR
                     VFPIVPMFHAFGWGLPYAAPFAGAELIFHGSDSSAANIGRIIEQERVTLTAGVPTIWK
                     DLLPLIERKDVDASSLRGIYVGGSALPRPLIDAYDKQGIEIVQMWGMTETGPLACISL
                     PRRGHRGLDRDGLLDQREKTGTIFPGLEARIVAPDGSVCPRDGKTVGELECRGPWIAS
                     AYYNDPEGSREKFHDGWLRTGDMAFMEPDGTFRIVDRAKDLVKSGGEWISSVELEGAL
                     LGHPLIRDAAVVGIRSRKWDERPVAVVVPAGDEHPTLESVREFLTPLVAKWWLPDGVV
                     IVDEIPKTSVGKIDKKVIRAQLDHMELP"
     gene            complement(19121..20155)
                     /locus_tag="VM841_06730"
     CDS             complement(19121..20155)
                     /locus_tag="VM841_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL89914.1"
                     /translation="MSALEQPVAAEALRVSGLRTGFGSNTVLHGVDFTVAEGEIVCIL
                     GLNGAGKSVTMKTIAGIVPAWDGQIAFFGRPMTTMGVEDRVGLGISHVTQGRQVFPEL
                     SVEENIRLGGYLLRRRDRERYTRNLAAMYERFPRLGERRDQPAGTLSGGEQAMLALAR
                     ALVSQPKLMLVDEPTAGLAPVVVDELAEALTQVNRDGVTILLVEQHVAFALRLAHRAL
                     IMQRGTIVYQERVAGIDHETMVQALGIGRLLDAPAARKKAPAREVPATALVKKVDAKK
                     TAASTKKTAASTKKTAAPLDKKAAPTKKTAAPTKKKAARAKKTAAPANKMAAATKKAP
                     AKKAPAKKAP"
     gene            complement(20152..22008)
                     /locus_tag="VM841_06735"
     CDS             complement(20152..22008)
                     /locus_tag="VM841_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVL89915.1"
                     /translation="MTDMVQRLRGAGLPVVSKRTRTILIVLAVCVIAPPLLARADILS
                     SFRASVIGYGACFALMAISLNILMGYAGQISLGHAALLGIGAYTSGLLTGRLGWSYLI
                     TLPFAAGAGALLAFVAGLPALRLRGLYLAITTIGLVFFAENTLYQLPWQGGGPVMLPR
                     PVAGRFIFLIDGDFLALILVTLVAFWWLDVNLVRSRLGRAFHAIREDEQVAQSFGIDV
                     TRFKLLAFVISGALAGIAGMTYGHLVGTVASDVFDYQFSLLLVTFVVIGGLGSRAGVV
                     IAAFLFGFFPRLLTALKDWDLLVGAFLLIYTLAKHPGGFAGAIREGKEAKAAKAARAG
                     LAGDTDEQAVPALPRTGLAPAAVAADAPPLLEVRDVQVAFGGLRAVDGASLTVPRGRI
                     VGLIGPNGAGKTTLFNAISGFVKMQAGQVIYDGTSIQDLPPHQRAAMGIGRTFQLVGL
                     ARNLSVRENLLLAQHSHASYGTWHALAATPRRTREERELDERARAAIASLGFERYADV
                     PVKHLSGGQQRIVEIAAALSTAPEMMMLDEPSAGMAPAVIESLAQRLRELRDDLGRTV
                     LLIEHHIPLVLDVCDEVYVLNLGKVLAHGTPAEIVSDPTVVDAYLGSAGVKA"
     gene            complement(22005..22880)
                     /locus_tag="VM841_06740"
     CDS             complement(22005..22880)
                     /locus_tag="VM841_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL89916.1"
                     /translation="MGSALISGIVVGGIYGLIALGIVLVYRGSRVLNLAAIETGTLGV
                     FMAWALIEEAGMPWMVGALAGVAAAALVSFAFERLVVSRMMDAPRLTVAVATIGLGAF
                     LMAFEIKVWTAAPRFLSHPIEGLGPKILGVHVSPTQILSLIIVAALGFGLAWFLRRTD
                     FGLGVLAAAQDPVATQMVGVRLWKVSAFTWTLAGALSAIAMLLVEPSIGGFAAGTLNG
                     LFVPALAAALVGGLTNPSGAFLGGIVMGLIDQVVRQWFLDVTWLPGVGTMAIFAAMLL
                     VLMLRPQGLLGRART"
     gene            complement(22888..24354)
                     /locus_tag="VM841_06745"
     CDS             complement(22888..24354)
                     /locus_tag="VM841_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL89917.1"
                     /translation="MRRFRSVAALVALGMVTVACSGSLPDWHYGAKANDPQVTIVDPD
                     EEPVAGGPTENGTPLPGTTVLPTAIPSGGVTTTPLPPTGGPVPTNRTTDRPKQYVANI
                     YKGSADTIGITDSQITLCGHAALVLAAAFNISAADLSVYWQQVNDTADKIHGRRVAIS
                     YEDDRYDGTEAAAVVKRCVDKKPFFILGGIGFDQIPFAREEAERQKTLYLHHMAIAKG
                     MERAVHSFSTQPTVEQVGVAFGEYIATRHKGKKLGIISRNSEYWKPGHDSGVAELKRR
                     GITPAKDLETIKNQGTYNLEIRELQNADVKVVWIWENALAAGQIIRQAAEQGYRPKWI
                     VFPFQTTLDLIGKNNALGPTIEGVGTWSAYTSGGYGGAFSQYGYDDEIKRFEAAYRKY
                     RPGVTPNDILWQVWIGNKFLHQMLLDCGRTCTRNRIAGTFLAGLKTRINPNCETNFAH
                     KKSMGGRVGLHQFVTLETFDRGQNGAWKTTTWCKESLG"
     gene            complement(24422..26344)
                     /locus_tag="VM841_06750"
     CDS             complement(24422..26344)
                     /locus_tag="VM841_06750"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL89918.1"
                     /translation="MDNSDTQAPSVSITSPTGGTVSGVITINASASDNVGVTKVEFLV
                     DGALLATDTASPWSATWDTSAAANGTHTLLARAYDAAGNSTASQQVTVTVTGGVEDVT
                     PPTVNITFPTNGSTVSGTVTISASASDDFGVTKVEFFVDGASIGTGTPAGSAGPWTIP
                     WNTTSVSNGSHALMAKAYDAKGNVGIDNDTTVTVSQTVAVLDETFSNRDGNGDYYDTT
                     GWTAGGYVSSTENHTNAAGNSAFGYASSGVSCATGNKTQTLSRSVTLPNNPVLSYWRK
                     LDLKALINTSTTASFKVKVNTTTVDQQAVTYANYVDSNWTERANLSLSSFANQTVTLA
                     FEVAANSNVCIEAWGKAWLDDIKIGNPQQAADTTAPTVNVTAPANGATVSGAVDITAT
                     ASDNVGVVKVEFYVDGMLLGTDTSSPYALTWTTGSVPNGSHRIMAKAVDAAGNVGTDD
                     DTTVTVSNGGGGTTSVTFGNDDANDGYVKANASGGSAAVGTLESTYGLAIGRGSDALY
                     NRTVLSFNTASLPDTATITRAWITVTLNSTSGDPWANPAGNTLVIDVKNGCFGACTIE
                     TGDWSSAPTQSAVSNIVKWTGGTTSSTNFSSAGLGAISKTGTTQLKLRFSSNQTATNY
                     IFIGRGAAATLHVEYT"
     gene            complement(26342..27289)
                     /locus_tag="VM841_06755"
                     /pseudo
     CDS             complement(26342..27289)
                     /locus_tag="VM841_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
     gene            27471..28154
                     /locus_tag="VM841_06760"
     CDS             27471..28154
                     /locus_tag="VM841_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL89919.1"
                     /translation="MALGREAAKAVARKAAPAGRAVQKPAAAAEPARRLTKGERTRAR
                     LVAAAEQVFEQGSYDEASIVEITRQAGVSQGTFYLYFPSKLEIFREVVRDLSHQLRSH
                     IAQTVEGAATRAEVERQGFEAFFAFVQQHPGLYRIIRQAEFVDRDIYRYHYERLAEGY
                     TRGLRAAVKAGEFRDVDVEVLAYALMGMGELIGARWLLWEDEPGGKVPAKVVDALVGI
                     VLEGVKKPN"
     gene            28408..28863
                     /locus_tag="VM841_06765"
     CDS             28408..28863
                     /locus_tag="VM841_06765"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HVL89920.1"
                     /translation="MLYKILLFVHVTGVVFWVGAGGAFQIMAERVAKMDSSRVKAFIQ
                     TGSLVPSAYFGILTAVVLVSGVWMVVDSGIGFEQPFVVAGVVGLIASGAIGGAVMGKT
                     SASLYTLVASPNFDEKVMNAGVAKMRTFGRLDLAIMLFVIYMMTVKPGT"
     gene            29098..29547
                     /locus_tag="VM841_06770"
     CDS             29098..29547
                     /locus_tag="VM841_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL89921.1"
                     /translation="MSEIKESVEVDVPVNVAYNQWTQFEEFPQFMEGVEEVRQLSDDR
                     LHWKAEIAGTEREWDARITEQVPDQRIAWVSEGGEANAGVVTFHKIDDNTTRVMLQLD
                     YEPHGMLEKAGDMMGMVKHRASGDLKRFKDFIEGRGRETGAWRGNIS"
     gene            complement(29580..29885)
                     /locus_tag="VM841_06775"
     CDS             complement(29580..29885)
                     /locus_tag="VM841_06775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HVL89922.1"
                     /translation="MSKVSMVGKITVLPGKADEAEPVLAKMVAAAKGEPGVEVYSYHR
                     GEGDTFWFFAVMADEQAVAAHRQTPAMMAAMGEFMPMAAGRPDMSLATPIAAIGFDA"
     gene            complement(29928..30209)
                     /locus_tag="VM841_06780"
     CDS             complement(29928..30209)
                     /locus_tag="VM841_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89923.1"
                     /translation="MKELPLIACSLDASGQKERAAEWRALVARSRGAEMRPDGLHIRV
                     EPGDDAEARRLADLESRCCPFFTFSFEPGGFTVGGSPDAVTAAKRLLLG"
     gene            complement(30221..30616)
                     /locus_tag="VM841_06785"
     CDS             complement(30221..30616)
                     /locus_tag="VM841_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89924.1"
                     /translation="MNGRLRSAAWLYAAGLALHTADHLRRGADVITPQVKMIGAISTV
                     AGGIAIALVLVRDRRGSTAAAWTGLPVAIGIAAVHLLPRWSAFSDAFVGASAVGVNAL
                     SWFAVIVEIAGAAAFGLAGIHARRPRLAG"
     gene            complement(30613..31767)
                     /locus_tag="VM841_06790"
     CDS             complement(30613..31767)
                     /locus_tag="VM841_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HVL89925.1"
                     /translation="MSEITIVGGGLTGLVAAIAAAEKGASVRLLEAQRRLGGRARTTA
                     PPYRANFGPHVIYGDGPLWRWLAERNLTPPHSGPALSGFRFIAGGVVRKMLPAAAVTT
                     MTRLGRAEPPADVDLRTWLSAKTDERTAAMLCAAAGVVTFDHDPGRLSAAFAWTQLRR
                     VAVSARHVKYVHGGWGSLIDVLARHATSIGVRIETGVRVDQVEPVSIVATRLHAACDL
                     LRDETLRWESARCALLDVALESRRGDPFLVWNLDRPGWIERYTKPDPSLAPPGVSLLQ
                     AQAGLEPGIPLEDGIAHLEAMLDASFPGWRERAVWSRSHISDGKTGALDLPGTTWQDR
                     PAIDRGGGIFVAGDQVAAPGLLSEVGFASAIEAARLACVAASAARAGGPR"
     gene            31806..32465
                     /locus_tag="VM841_06795"
     CDS             31806..32465
                     /locus_tag="VM841_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL89926.1"
                     /translation="MTVRLSQRPGKSRATQTERRARSRTALLEAAARGLSRYGYGNLV
                     LEEVAREAGYTRGALYHQFADKEDLAMAVLRWVDETWRDEVGKQVEGETDPLAILTTL
                     ARGHAIFCRRDIARVANALRMEFSGRDHPVGREVERVSQTLIKRVMKLIDAGRRQGSI
                     PPGPPSRAVAHALVGALEGAVIELAGHAPHDEKLAVRAALGVLGLNPAVHETAMKGRP
                     R"
     gene            32462..33205
                     /locus_tag="VM841_06800"
     CDS             32462..33205
                     /locus_tag="VM841_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVL89927.1"
                     /translation="MSDLRTDHALFRLRDAFPRARRDDTLPPGQRAISTFPRYGIARP
                     DPPVPAHPMIQIMGAQDAVLELDADALNGFPRVRVAADFHCVAGWTARDLRWEGVRFR
                     DVYDALSERAPVTHILVVGRDGWRATLLLEDALRDDVLIADRLDGEPLPLDHGGPFRL
                     VSPAQYGYKNVKHLVRIELHTSEPNDVVGGYFDRLLFRTLLAKHPRARVAHEERHRSM
                     PSLTTRRIYTKLHPITYLLGYIGARRNPR"
     gene            33220..>33683
                     /locus_tag="VM841_06805"
     CDS             33220..>33683
                     /locus_tag="VM841_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVL89928.1"
                     /translation="MSEIILFHHAQGLTDGVRAFADELRAAGHTVHTPDVYDGKTFDT
                     LEDGIAYAKEIGFGTALERGVAAAEGLSAEMVYAGFSLGAMPAVKLAITRPGVKGVIL
                     MHSVAPPSEPQTWVAQAYLITFGTVGVFGLYLFLLSRWTASAVSYEFVLAPLA"
BASE COUNT         5262 a        11440 c        11585 g         5396 t
ORIGIN      
        1 gtacagggcc tcgcggtggg cgccgcatcc gttgtggcgg tcacggttgc cgcatcgctg
       61 acgctgctgc cggcgctgct gggcttcgcc gggcagaaga tcgagctgac tcgctggcgc
      121 ggcctgatcg cggcgggctt cgtcgcgatc gcgctggccg gcgtcggtct ggacatcacg
      181 ccgctgctgg tcggcctccc ggtcgcggcg gtggttctga tcgcgggcct gttcgccgct
      241 ccgctcaagc gcgaggtcag gcatcgttcg cgcaaaccgc ttcgcgagac cgcggcgtac
      301 cggtggagcc gcgtcgttca acaccatccg tggaaggttg ccctgatctc gtccggggtg
      361 ctgatcgcgc tcgcgctgcc gttcttcagc ctgcgcctgg cgttttcgga cgaaggcaac
      421 tacggcgagg agaccagcac ccgcaaggcc tacgatctgc tcgccgaagg attcggtccc
      481 ggcttcaacg ggccgatgat catggcggcc gagatcccgc ggggcaccga ccgggccgcg
      541 ttgcaggccg tcactgatgc cgcacagcgc gaccccgggg tcgcgttcgt ctcgccgccg
      601 gtcatcagcg aagacttcag cgcggtgctg tggcgtctga tcccgacgac tgcgccgcag
      661 gacgaggcga ccgccgcgac ggtccggcgt ctgcgcacgc aggtcctgcc accggccgcg
      721 agccgccttg gcatggacgt caccatcacc ggcgcggtcg cggtctcgca ggacttcggg
      781 aactatctgg ggtcgcgcta cccgtacttc ttcggcgcgg tgctgatcct ttcgttcctg
      841 ctgctgatgg ccgtgttccg gtcggtgctg gtgccggtga aggccgtgat catgaacttg
      901 ctgtcgatcg ccgccgccta cggcgtcgtc gtcgcgctgt tccaatgggg ctggctgtcc
      961 gcgatcaccg gggtgcagcc cgcaccgatc gagccgtggg cgccgatgat gctcttcgcg
     1021 atcgtgttcg gcctgtcaat ggattacgag gtgttcctgc tgtcgcgcgt ccgtgaagag
     1081 tggcgccgca ccggtgacag ccgccgctcc gtcgccgacg gcctcgcggc gacggccaag
     1141 gtcatcagcg ccgcggccgc gatcatggtc gtggtcttcg gtagcttcat cttggagcag
     1201 gatcgcgtga tcaagctgat gggaaccggc ctggcgatgg cgatcctgct ggacgcgacg
     1261 atcgtgcgaa tgctgctcgt gccggccacg atggagctgc tgggcgacaa gaactggtgg
     1321 ctgccgcgct ggctcgacaa gattttgccc cgtgtggacg tcgagggaac cgccacgccg
     1381 gtcgatcaga tcgacgagcc ggttaccggt gtgcggtagg cagccaggcc cgagcggatc
     1441 gcatcgacga cgccgtcgcg tcggcgtttc acgtcttcgt cggtgacgtg cagaatccgc
     1501 catccggccg acgtcagcgc gttgcgtcgt gccagatcgc tttgccaaac gtcgcggccg
     1561 gcgtgccaac ggtatccatc ggcctcgatg ccgaacatcg catccggata ggcgaagtcg
     1621 atgaacgcgc gccgggcacc gagcaggatt tcgcattgcg ggatcggccg aggcaggccg
     1681 ctggtgacga tcagatcgta gacggcggac tcgagcgggc tttggagaag cgccggaagg
     1741 ccgtcccggt cggcggctgc cgcacggacg acctcgatgc cgcgccggcc ccgctgggcg
     1801 agcgcgtcgg cgcggcgcat gagccgggga atcgtaatca ggcggcgtcg aagcgcatca
     1861 tccagcgccg cgccgacggt cgcgtccggg acgaccgcgg ccaggtcgat cagcgtgcgt
     1921 gccggcgagg tcaccgggat ctccccgatg tgcgtgacat cccggcggtg cagggccgag
     1981 cgatgagcga tgaagtcccg gcggccgcga tacgtcggat gcgtgatctc gacggccgcg
     2041 tcggaacagt cggggaagcg ccacaggcga gcggcggcgc ggtgggacgc ggccgagccg
     2101 gccccggccg acagcagcgc catgaacatc tcgctgttcc acgacagcgg atgtccggcg
     2161 attgcgtaga tgccgtgtcc gacacgcagc cagcggcccg catcgcatcg acgctggatc
     2221 atgcgtgccg tcgcacccag gggcagggcc tgaccacggc tgaagacccc gaactggcgt
     2281 gcggcgagcc tcgcgagcgc tgcatcaacg gtttctgtgg acattcgagt acccatacgg
     2341 ttgccgaatg accaaagaaa gcatggcgcg gcggaaacgc caaaggcctg gcgggagctt
     2401 cggcagggtt gcgaactaat ggatgagtgc gggctccggc gtgctcgcgg catcggtgcc
     2461 caggtaggcg gcgataacgg cgggatcgct gcggatctca gccggcgtgc cggtcgcgat
     2521 gatgcgacct tggtccagca catacatgcg gtcggacagg ctcatcacga accgaacgtc
     2581 gtgctcgacg atcagaaggc tcagcccgag gccctcgcgc agcgcgacta gcagctcgcg
     2641 cagccggtcg gtctcgcgct cgtccaggcc gctcgcgggc tcgtcgagca gaatcaggtt
     2701 ggcgccggtc accagcgcac gcgcaatctc gaccatgcga agcacgccga acggcaggtc
     2761 gccgcagcgg gcgtcgagca ccggtccgag cccgaccgtg gccgcggcgg cgtgcacgcg
     2821 agcgcgggcg gcctcctcgt ccttcaacgc cttgtcggtc agcagcagat gagagcccgg
     2881 cccggtcgtg ccgtgtaggt gcgtggcgac catcaggttg tcgaacacgg tggattcttt
     2941 gaaaagctgg atcgcctgga acgtgcggcc cataccgagg gcggcgcgct cgtgcacgcc
     3001 cagcgcggtg acgacccgtc cagcgatcga gacgctgcct gcctgcggcg agttcatccc
     3061 accgatcgcg ttgaacatcg tggttttgcc ggcgccgttg ggtccgatca gcccgacggt
     3121 ctcgccgcgc tcgacgcgca gcgaggcctc accgaccgcg gtcagtcctc cgaaacgcac
     3181 ggtgatcccg gaagcctcca gcgccggcgg cggcgggccc tgtccgttcg cggacggcgg
     3241 cgccgcggcg gcgggaagct gcggcggatc aagcatcgcg cgtggcgcct cgacggccga
     3301 tcggcgcgcg agcagctttc ggaccggctc ggcgcgcgcg aggatcgcga tgagcagcag
     3361 cccgccggac accagctcga ggtagccgtt gaggaacgtc aggcggaaga agatctcgtt
     3421 gagcgcggcg aacagcgcgg cggccgcggc ggcgccgccc agcctcgtca acccgccgac
     3481 cgcggcgatc gcgagataga acagggacca cttgaacgag aattgatcgg cgttcgcggt
     3541 gcgctggtcg atcatgatca agttgcccgc gatcccggcc agcacgcccg agacggcgaa
     3601 cgcgaacagc ttcgtgcgcg tgacattgat gcccatcgag gcggccgcaa gctcggaacc
     3661 gcggacggcg aacatcgcgc ggccggtacg cgagtcgcgc cagtttgcgg ccgccgcgat
     3721 cgccagcgcc gcgcccgcga gcgccacgta atagaagatc cgtcgctgcg tcaggtcgaa
     3781 cgagggaaag gcgccctcgc gacccacgac ggtgttgccg atgatcgtcg agcccccctc
     3841 gccgacgagc caggcctgtt tgaacagata cgcgtcggcc atccatccga agatcagcgt
     3901 cgcgaccgcc aggtacagcc cgcgtacgcg cagcgcgacc acgcccagca gcgcggcggc
     3961 accgcccgcg atcgccgcgg caatcggcaa gttcagcggg aacgggatcc cgaacttctt
     4021 gaccatcagc ccggtcgaga acgcggcgag ccccacgaac gaggcctgcg cgagcgagat
     4081 ctggccgacc cagccggtca acacgacgag cgagaccgcc gcgatggtga agatcgcggc
     4141 cagcgcggcg tcgccgtgca gcgagtcggc cagcccggcc atcgacgacg ggaacggcac
     4201 ccacggccag gccaacgcga ccaccagcgt gacccacacc cacggcgagc gaagccgcag
     4261 ccgcttcggc ttgcggatcg cggcgccacg gccgctgtcc tcctcggtgc ccgagatctt
     4321 cttgccgcgt agcgccatga cgccgagcgc caggatcgtg agcaccagct gtccgacgcc
     4381 cggcgaggct gtgaatccgc cgacgacgcc ttgcaccagg cccacgatgg ccgcgccggc
     4441 gagcgcgccg ccgagcgatc cgagcccgcc cagcagcgcc gcgacgaatg cgggaagcac
     4501 ctgccgcgac agtgtgtagg gctcgaggtt ggtgacgccg gcgagcagca cgccggcggt
     4561 cgcggcgagc gcgccgccca gcgcccacgc cgccgaggtt gtgcggtccg ggtccacacc
     4621 catcagcgat gcggctcgcc ggttgtcggc cgcaccgcgc atcgcgagac cgatgtcggt
     4681 gtactgcagc atcatcgcga tcgatcccgc ggcgatgagc gcgacgagcg ccagccccat
     4741 ctcgccgtac tgaaggatcg agttgcccac gtcgatcgag ccatccggga agatcgtggg
     4801 cgcggccagc ggggccgtgc cccagacgcg cgcgatcagc gcgacgagca cgccgagcac
     4861 cgcgacggtc ccgaccgtct gggtggtctg cgactcgctg cgaagcggcc gcagcacgcc
     4921 gcgctcgacg atcatgccga gcaccgcgcc cgaggcaatc gccagcggca gcaccaggaa
     4981 catgggcacg ccggccttgg acatcgagta cgcgaggtag gcgggcaggg tcgccatcgc
     5041 gccgtgcgcg aggttgagga tgcgcgaggc gcggtagatc accacgatgc cgagcgcgaa
     5101 catcgaatag gcgcccgcca gtggcagcgc cagcaggatg ctggtcagga tcttcgtcac
     5161 cgtttgggcg cgtccatgcc gacccacggg tcggcgatcc agccggtgcg ctcgtcgcgc
     5221 cacttctcga acgagccgtt gttcatcacg acgctgaagg cctgaacgct gttgttggcg
     5281 aagtggttgc ctcgccgcca gcgcagcggc tgcgtcagcc cgtcatcgag cacggtcgag
     5341 tcgagcacgt cgcgcaggcg ctcgcgggtc aggtccgggc cgacctgacg cagcgccttc
     5401 accagaagct gcatacccag atacccgccc tcgacgaacg ggttgttcac gtccacggtc
     5461 gaggagttcg cgcgcaggtc attgacgtag gcgctcacgg caggcaggtc gtcgaacggc
     5521 gggatcggag gcgtgtagcc ggtccagacc cacaggccgt tgcacggctg tccgcacgcg
     5581 cgcgcgaacg agtcaacgaa cagcggctgt ggcccgccgg tgccgcccgg ctgcatccca
     5641 ccgaacgacg cgccgtcgcg gatccactgc agggccgtcg gcggctcgag cagcatcgcg
     5701 acgaaatcgc agggattgca acgaccgttg aaccggtcca cgtcgttctt gtacgagggc
     5761 tggccggcct cgatgccgat gtcggccttg atcgcggcgg tcgagcccat catccggcgg
     5821 atcgcgccgc ggaacgccga ggctccctcg accccgaagt ggtaggactt gtcgtagacg
     5881 agcccgaacg tcttggcgcc gcggtcgcgc gcgttcttgg ccatcacgtg catcgtggtg
     5941 atcgtggagg cggcgaccgg ccagacccac gggtcggtgt actggctgat cagcatgccg
     6001 tcggcgccga ccaccgggat gccggccttg ctgatgtcgc cgttgcggat cgcggcgtcg
     6061 aggccctccg acgatggcga gaccgcgagc gcgaacacgc cctcggagat gaagttgcgg
     6121 atgaactgct ggccgcgtgc cgcgtcccag ccgtcgtcga ccatcttcag gttgagcagc
     6181 cggccgcaca cgccaccgcc gcggttgact ttgttgacca cggcctgcag cgcgatcgga
     6241 acctccttca ggaacgacgc gccgatgccg gtcttgacga cggtcgcccc cagcttgatc
     6301 gaggagttgg tcacgccggt gtcggtcgcg ccgccgttgc ggccgcgcac gcactccagc
     6361 gtcttgccgc cgcgcacgac gggcgcggcg ccgggaccgt cgcccggatt ggccgtcgcg
     6421 ccgggcgccg ccgacgggtc gcccggctgc gacgggtctg tcggcgcacc gtcctgcccc
     6481 acggtgtagg tgcgcgtctc gggcttggac acgtagcggg tcgcgaacga caccgtcgag
     6541 agcaaaacca ccgcgagcag cgcgggcaag gctttgaaga tcttgcgctt catcgtgctc
     6601 ccacgtgccg tcgacgcagg atcaggtagg cgggcagccc gaggaacaac cacagcgctc
     6661 ccagcaggag cgcatccttc ggcttcgcga gcgcgagcgc cgaacccgcg gcgatgcggc
     6721 gcacgaaggt ctcggtgacg gtcttggttc ctttgacgac cggcgccggc ggcggcggga
     6781 ccggtgcgtc gaccgtcggc tcgacggtgg ggaccggctc gacggacggc tcgggctcgg
     6841 gctccggctc cggcgcatcc tggccgagca ggtagatgcc gtagtgagac tcggcctgca
     6901 cgcccgcgag ctgaacgacc tgacggctgc ggccggcgcg gccgtcggcg aagaacgtga
     6961 cctgcatccc ggtgacctcg ggcgcgtcgt catcgttgag cgtcttttcg gcgtagcccc
     7021 ggaaggtctc cttctgcacg acggcctgat agccgcgctt cgagccgccg aaccacgcgg
     7081 tatcgggcga ggggaactcg accttgacct ggcccggcaa cgcctgctcg atcgcggctg
     7141 cgacggcctg tgggcggcac agctcgcatg tgaagtccac ggtcccgttt ccgtcggtgt
     7201 cgatgcgcac gttctcgatg aaacgttcga acgaaccggc ggcggtgccc ggccgtccct
     7261 tcgcgcgcgt gagcgcctcg gcacggatct ctccgatgcg cacgcggttt ccgatcgaca
     7321 cgtcctgcac gaccgcgatg gcgcgggcga ccaggccgtc gcgctcgttg cgcgaaacgt
     7381 tcccgaacgc gaccagggcg cgggtggaca gcagcgcgcc ctcgtcggtg gccgagccgg
     7441 tcagcgccga tgcccccgtc accgacgtcg catcgaggtc gcaacttgcc gacgagcccg
     7501 cctcgttctt cgattcgggc cggccggcgt cgtcgcggca ctcggccgcg gggaagggcc
     7561 aggcccggcc ggcgcgctcg gcgtcgcgga gcgtgcccgc gtccgcgtgg ccgaacgatg
     7621 cggcggcctc cgacgaagca ttgccgatcg agacgttgtg cacgcgcgcg gcgtacaggt
     7681 cgcggttgga ctggtcgagt ggaaccgtcg cgaccgcggc ggccgcggcg tcgaagccgc
     7741 ggtcctcggg cgagaactgt ccgagcgcga gattccagac catgcgctgc gcgccgcccg
     7801 tggtgagcgc gcgcatcgcg aacgcgcgcg caccgccggt gtagttcacg tcggtcacgc
     7861 cttttcgctc ggcgacgtcg gcggtgagcg cgtcgggatc gccgggcggc gggtccttgg
     7921 acacgcggat ggtgtccttc aggatcaggt actcggtgga ttggccctgc gggttcttca
     7981 cgaacgggct cggcaggaag acgcgacggg tcaccggatc gacgtgaatc gagccgatca
     8041 cctgtccggc gagctcgcgg aagagcaggc cggcgggcag gggagtgcgg cgcgcgtcgg
     8101 cgatcaggaa acccgcgggc gtctccatgt agagccgtcc ggtcgcggga tcgatgccca
     8161 tcgcgtagtc gctcgcgccc acgcggttgt cgcccgaggc gatcacgccg agaaacgacg
     8221 agcgcagccc gtcgaaaacc cacgcgccgc gacccgcacc gccgttggtg gtcaggaaga
     8281 agacccggtc ggatcccgcg tcgaactggg ccgagagcgg accgggcacg ccggggaaga
     8341 cctcgacgcc ggccgggccg gcggcggtca acgtcacgcg cagcacctgc gcctgcacgt
     8401 tgcccggccc gtagcaggtc acgtagatgg tgccgggacg cagccggctg cgcgcgagca
     8461 cgggcgcgta ctgcatgtcc tgcgcgttga agcagtcgtc gagcagatac tcgaagcggc
     8521 gagtgccgtc gagggcacgc tcctccagcc acagacgcgt acggcccatc acctcggtcg
     8581 gggcgcgcgt cagcaggtag acggtcttgc cgccgtcgtg cgtggcgagc gcgagcggaa
     8641 agcgcgtcga gcccggccac agctgcggtc cctgcgtgac cttcttggtg cgaaggtcga
     8701 tccagatcag ccggtgcgcc tccaagcgaa gctggggatc gacggtgcgc tcgatcgcga
     8761 tgatgcggcg ggtggactcc tcaagccgcc attgccattc ggtcggaccc gaagtggtga
     8821 cggccggcat cagctcgggg atctcggtcg tggagagcag cttgagcgag tcgaggtcaa
     8881 cctcgaacag cagcattcgt ccctcgcgca gcgacgcctg ccatccgcgg cgagccttgg
     8941 cctcgatcag gatgttgccg ttggcggcga tcgagtccag gccggtacgg cgatccagcg
     9001 ttccgacggt ccgcatcccc ccgtccgccg tggccggtgc gtccggcaag gccgtgaaca
     9061 cggcaggcgc gaccacggcg accacgacga gccgaagcaa agcgcggcga acccggaggc
     9121 gtggcgcccc cgggttcgca gccttcccga ttccctgcat cgggttcctc tcgcctttgg
     9181 ttgtggctag cagccgtcgc cgttcgtgcc caggatcgga tcctgggtgc agaagtgcca
     9241 gtccggcctg gtgcggatca cgcgcgccgg caggttggtg ctcacgatcg cgatgccgtt
     9301 gaaggtgatc tgggtcaggt tgccgcccac gtcgcatgcc gccatctcgt cggttttggt
     9361 cggtgccgca ccgaagtaga agcggatctt gctcggggcg tggcccgccc gcgagatcac
     9421 gccgctgaac tcccacgcgc cgcgtcctcg tggaaagccc attcgattga tcgtccagtc
     9481 ctcgccctgg aagcggagcg ttccgccctc cgccccgccg taggtctggc cgcagtagcc
     9541 gtagaaccag gtcggcgcgt tgatccggca ggtggtatcg ccggtcgtcg cgtcgacgca
     9601 tccgccgagc aggtcggtgt tggccgagat ctgacccatg cacggcgggt tgagcccgac
     9661 ctcgccctgg gtcgtatccg cggccctcca gtacggacgg taggtgccga ggttgcgctg
     9721 caggcggcat gtcgcgatct gcacgccggg ggcgttcggg tcggcgtcac ggcagaccgg
     9781 cggaagctgc gccggttgcg ggatgccgag gtcgtcgcgg acggggttgt tgccgacccg
     9841 cttccattcg tactggcagt agccactgat gttggacgtg ccgtcgatat acatgacgtg
     9901 ctcgccggcc ggatgagccg acgaggatgc cggcgcgaac atcgagccca gcgcgagccc
     9961 gatcgccagc atcttgtaga ccaccttctt gtgccgcatc gcgtccctcc ccttcgctga
    10021 cggtgccccg gcgtggcctc cccggcgggc cggaacctgg tgtccggttc aggtgtcaga
    10081 aaacctgaac cgcctctcag ttttgtgtgt aagttcgacg tcttgccgtt tcgttcctgc
    10141 ttcgaaaccc ccgcttcttt cgttgggacc cggtctgctt gctacgccgc cgcgagccag
    10201 gcctcgacct ggctcatggc cgttgagttc cagcccagtt tcgagtggtt gatgctcagc
    10261 gtgaccgtgc cgcccagatt cgcgatcccg gtcgaggacg tgcacgagtt cttgaagatc
    10321 agcccgtcgc tggtcccggt gtgctcgttg tggatgttcg ggatgtcggc ggtctggccg
    10381 cacagcagat atgtgcgcac gctcgacggg atgccggcgc tgcggatggt gtccaccagg
    10441 ctcgagccgg tgtaggcggt gatcccggcc gagtacgaga cgtaacccca gccgccgtag
    10501 taggtcgtgt agtagtcctg ttcggcgacc ggcaggccgt aggtgccgtc ccaccgcttg
    10561 agcatctgct tcgaccccgg gtaccacgag gagtcgtagg cgtactcggg gtggttgtac
    10621 caaacgccgt agcagaccag ccaggtgtgt ggcgccgccc cgttcgcgtt gatcccgcac
    10681 tcgggataga tgccgaccga ggtgttgatc ccgtgccgga acgcgtagtc gaagccgttg
    10741 ttcggagtgc ccagcaggat caggcgacgc acgttcccgt tgtaggcact gccccagctc
    10801 ctcttgacgc tcgagatgta ctgccgcgcg ttgaagccgc ccttggacca ggcgaccacg
    10861 tcgacctgcg acgctccggt cgcggccttg atgatctcga tcgcgtcgaa gatctgctcg
    10921 gcccagtagt agccgtcgcc gttcttgtgg gggaaggtga tgccgaagac tttgtagccg
    10981 cgcgagtcca ggtactgcat caggccggtg ctcgggcagg tcgatgcgcc gcacaggttg
    11041 ccgccgtact cgttggggtt ggcccaggcg aagtcggcgg tctggttggc gccgtgcacc
    11101 agcagcacgg gaaccggctt ggtcccggtg ctccactttg gggcgtagga aagcaggaag
    11161 cgtgacgagt gcggctggga cgagccgaag tgcgtgacgc gctggccttg ctgattcccg
    11221 cggccgtcgc tcggccagcc ttcggcggtg aagccggtcg agttgtcctt ccagcgttcg
    11281 accttggccc agccgaacga gacgcttgtg aaggtcgctt cgagcgtgcg ggacgaggtg
    11341 gcatgcgcgg gcgtgcgcag cgatgcgaga ttgccgagcg aacctgccgc gacgagcgtc
    11401 gcgacggcga tgcggactgc aacacgggaa cgggtcacgt tgggtcctcc cccggttcga
    11461 aaactccctg gcgttttcgg cgatgcggct acgcggttct cacctccctg atcgcgtcgc
    11521 ggccgccggc caggaaatcc gccacgatgc ggttgaactc gcgggcttgc tcgatgaaga
    11581 acagatgacc acccggaaga gttgtgatgg ctgcgccggg aacgcgctcg gccagcagcg
    11641 gcgtgttgcg cggatccacg accgcgtctt ccaggccggt gatgcacagc gtctcggctt
    11701 cgatgacatg gacgcggtcg ctggcgtcga aggtcgcgcc ggcgttccac tgcgccatgt
    11761 agctctcgag cggctgattg gattcgcgcc ggaagtcgat caccgtctgc acgacatcgg
    11821 gccgctgcgt cacgaactcg tcgctgaacg cgttctcgat gaagcgacgc agccgttcct
    11881 cgtccggcaa ggtcggggcg agctcgatca gatcgagcgt tcgctgcggc atcggcacga
    11941 agtccgcgcc gccgaacccg gtacacgcca gcaccagccg ttcgatcagc tccggccggg
    12001 ccagcgccag ctcttgtgcg acgaagccgc cgagcgaggt gcccacgacg tgggcccgtt
    12061 cgatgccgag cgcgtccatc aggccggccg tgtcggcggc catctcggcc gcggtgtagg
    12121 ggccgggcgg cgcgtccgag ccgccgacgc cgcggttgtc gaatgtcacg acgcggtagc
    12181 cgcgctcggc gagcggcccc gcgttccagt gccatcccca ccggccgtag gccaggccca
    12241 tgatcagcac gacccagggc ccctcgttgc cgtggacctc gtagtccagc gcgacgccgg
    12301 ttccggtatc gatgcgggtc atgcgtcgct tccccagcgg atcacgctcg ccgcccacga
    12361 gtagccggtt cccgccgcga gcgtcaggat cgtcatgccg tcgcgcaggc cgccttcgtc
    12421 gcgcgcgacc gacagcgcga gcagcggatc aaccgcgctc atgtgcccga acgtgtccag
    12481 atagatgctc tggttctcac tcaggccgaa ctcgccgagc agcatgtggt ggatcgagcg
    12541 cttcatgtgg atcggcagca gcaggtccac gtcgttgatg ccgtagccgc tgcgctccag
    12601 cgcctgggac gcaaccttga gaaagttctt gagcgtgatc ggatcgagcc ggtccttcat
    12661 ctcctgcggg tcgcgcacgt cgaggtagtg catgcggcgg tccacggtgg catggctggc
    12721 cgggtgggcg ctgccgccgg ccggcacgcg gacctggtcg ctgaacgagc cgtcggtcag
    12781 gatgctcgac cccagcacga cgttgtgcgg gtggtcgcgc ctgagcagga ccgcgacgcc
    12841 gccgtcgccg aagttgatca tgaagcgggc gcgcttgttg gtgtagtcga gcagttgcga
    12901 ctccttggcg gcgccgacca gcaggacgct tctcaggtgc gggtcggcgg cgagcatgtc
    12961 gcgcacgacc ttgagcgcga cgggcgcccc cgccgagacg ttcaccgcct ccatcgcgaa
    13021 cccgtccagg ccgagcgcgt gctggatcgc gggtgcggcc tgccagaccg cgtagtcctt
    13081 ccagtgcgat ccgaagtaga cgaccgcgtc gatgtcggcg cggccgttgc gctcgacgat
    13141 gggctcggcg gcggcgatgc acatgtcgga gacgtgctcg tcccaagcgg cgatgtgctt
    13201 gcccttcaag ccgaagcgct cgacgacgat ctcttcgggg atgccggcgc gttcgccgat
    13261 ttgggcggcg gtcatgaagt gctcgggggc gtagtgaccg aggtcggcga tcccgaccgg
    13321 tgggtttcgg tagacggatg tcatgggctg ccgtacctcg ctcgtagctc cggcttcagg
    13381 agctttccgg ttgcgttgcg gggaagcgtc tcggcgaaga cgacgctggc gggaaccttg
    13441 aacttggcga gtcgctcgct gcagaacgcg cggatctcgt cctcggtggc ggacgcgtcc
    13501 gtgcgcagga cgacgatcgc gcggccggac tcgccccaac gctcgtcggg cacgccgatc
    13561 actgcggcct cttgcacggc cgggtggccg gccaaggttc gttcgatctc ggccgggtag
    13621 acgttctcac cgcccgagat gtacatgtcc ttcacgcgat cgacgatggt gatgtagccc
    13681 tcgtcgtcga tcaggccgac atcgccggtg cgcagccatc cgccgcgcag cgccttggcg
    13741 gtctcctcgg gaaggttcca gtattcgcgc atcacgctcg ggccgccgac gacgatctcg
    13801 cccgtgccgc cgggcgcaac ctcgttgtcg tgctcgtcga acacgcgcgt gtcggcgaag
    13861 aagtacgggc ggcccgccgc gccggctttg cggatcgcgt cctcgggcgc aaggatcagg
    13921 cagttcggag cggcctcggt caggccgtag ccctggatga actgcaggcc gcgctcctgg
    13981 tagcgccgga tcagcggcac cgcgagcggc gcgccgccgc atacgaagga gcgcacgctc
    14041 gacaggtccg acgcggcgaa tcgtggatgg tcggccagaa cctgatagat cgtgggcacg
    14101 cccatcatcg aggtgatctt ctcgcgcgcg atcaggtcga ggacgcgtcc ggcgtcgaag
    14161 gcgggttcca gatacacggt cccgcccttc caccagaccg cgagcgtgtt cacgttccag
    14221 cctccgatgt ggaacatcgg cagcacgacc aagcttcgct cgtcctgggt gatgtcgagc
    14281 gcgacgatca tgttcaggtt cgtccagaag aagtttccgt gggtgagcac cgcacccttc
    14341 tgccggccgg tcgttcccga ggtgtagatg atcgcgagcg catcctcggg ctttgcgccg
    14401 gccgggctgc cggcgggggt cgcagccgat tgcgcgtcgt cccagcgaag gtcggacggg
    14461 tcggcggcgt cgccgctgcc cgcgacgaac gcggccttga tcgcggtgcc cggccggaat
    14521 gccgcgacgg cctcgcgcag cgaggcatcg tagagcagcg tcgcgctctg cgagtcgttg
    14581 cacaggaact cgagctcggg tgcggtcagg cgcgtggaca gcggcaccat gatcgcgccg
    14641 agccgcgcgc acgcgaagaa cgcgtccacg tagtccaccg tgttcggcag gtaggtcgcg
    14701 acgcggtcac caacctcgac ccctgccgcg gcgagcgcac ccgccgcccg cgcggcgcgc
    14761 tcgtcgagct ctcgataggt ataggaccca tccggcgtga ccagcgcgat gcggttgggc
    14821 gtcagcgccg cgcgccgcgc gatccaaaag ccgatgccgg tgtaggggaa ggtcattcgg
    14881 ccgcctccag gaacgtgcgg tacacgtcgc aggagacgac gacgccttcc gagccgggcg
    14941 ggcagaacga ggtgccgtca ttcgcgccgg cgatgccgac gaacgcgacc agaccctggc
    15001 gcagcgcggg ccgcaccttc agcgccttgc gcgcaagcgt gacgccgagc gagtgcgcga
    15061 cgatcgagaa ccgctccgat cccgtgtagg cgcgaaccgc ggcgataaag tcgagcagat
    15121 cagggacgtt gacatcgttg gaggtgacgc gggcgttgcc gtcccatccc atctcggccc
    15181 gttcggcgtc gcgctcgggg ttctgggtga acagggcgct gccattgttg gcgcccaggc
    15241 cgttgtagga aagagcccac aactcttgat cggtccagcc ggcggccttg aaccggtcgc
    15301 gtaccggata ccagtcggcg tgatcgacgt tgttgccgtg cacgaagatg accggggcat
    15361 gcgtgcgcgg cgcgcctgag gcgatgccgc cgaagccgcc gatgcgaaag ccccactcgt
    15421 ggtcggtcag cgccggaaag tcggtgggga agcggatctt cgcgggccct tgcggcgacg
    15481 agtgtccggc ttggtggtcc gcgcgggcgg gcagggccgc aagaagcgcc gcagcgcaca
    15541 gcaacgcgat ccggcgcttc atcgagtcct ctttcgaatc gtgagcgcgc cgaaggctgc
    15601 ggcgcccagc gtcagcgcgc ccgcgccgaa aagcgcgccg ggcacgccgg ttgcggcaag
    15661 ctcgcgcttg tcacccagca cggccgccgg cttcggtgcg ggcgtcgtcg gcgcggcaag
    15721 atacggcttc tcggcgtttt cgatgaaggc gcggtagtcg gcgacgacgt tggcggcgac
    15781 gcgaagctga ttgtggttgg tgaagggata ctgccggttg tcggcaccct tcagcacggg
    15841 actttgggcg taggttccca tgaaggcgac gtcgtaggtc ccggtgcctt cgtaaacggt
    15901 catccacttc gcggggggat aggtttcgtc gctgccgtcg gggccgttga gctcggccag
    15961 ccatggcgtg ttcgcggcga tctcgtcgca ggcgtaaacg ttgccttcgc taccgggcgg
    16021 acacaggctg gtgccgtggt tgccgccggc gatgccgacg aacgcgacca agtccgcgcg
    16081 cagcgccgga tgcaccttga gcatcttgcg cgcgaccgtc acgccgagcg aatgcgcgac
    16141 gatcgtgaac ttcttcgagc cggtataggc acgcaccgcg gtcacgaagg catgcaaatc
    16201 gggaacgttg acgtcattgg cggtgatgcg cgcgatgccg tcaccgccca tctcggcgcg
    16261 ctcggcgcgg ccggcagggt tgtcacgcga gggcgtgttg ccattgtcgt tgccgacacc
    16321 gttgtaggac aggccccaca gctcctggtc ggtccagccg gcggccttga agtcgtcgcg
    16381 cacgtgatac cagtcggcgt gatcgacgtt gttgccgtgc acgaagatca ccggcacgtg
    16441 cttcagcggc gctccctgcc ggatgccgcc gaacccgccg agccgaaagc tccactcggc
    16501 atcggtcagt gccgggaagc cggcggggaa gtgtgccttc atcgggccca ccggtggcgt
    16561 gtggccgttc tgatgatcgg cgcccgcgga tgcggccgcc agcagcagcg cgagccccgc
    16621 gacgaccgcc ctcacgcgcc gcacgccttc cggatgaacg ccgcgacctc ggcggatccc
    16681 tgcccgagca tcatcgagaa gtggctcgct tccagcaggt gcggctcaag atggggcacg
    16741 cgcgcttgca gcgcagcgat ctgctcgtgc gagtacaggc cggacggctc gttcatcacg
    16801 ccgcgctgcg cgcgcaggaa gatcgcgggc cgggtgatct gctcgatcgc cgtgacggtc
    16861 agtggatcgg tgagcgtgtc gcgcgagtcg gcgtagaccg cctccagcga tacgccgctt
    16921 cgcagcgcgg gctcgtcccc gatcaggtca tagttcacgt atgcctgcac gtgctcgttg
    16981 aagtcgccgg cgaacgcggg atggcgtctc cagaaatcct gatacgcggc ccgcgaggcg
    17041 aacgtggtct tgagccgctc catcgacggg ccgatgatct ggtgcagcag cgcgtcgatg
    17101 tcggcctcca ccggcgcgtc gagcagcttg atcccgccgt ccatgatcac caggcccccg
    17161 acgcgttccg ggtgacggac ggccgcgacg gcgaccacga acgcacccat cgaatgcccg
    17221 acgaacaccg cgttcggcag cgacaggtga tccagcaccg cagccagatc ctcggtgtgc
    17281 tgcgggatcc cgaacgggcc ggtgatcgcg ttcgagccgc cgcggccgcg caggtccggc
    17341 gcgacgacgg tgacgtcgcc gtcgagatgc tcggcgatca gcgaccaggc acggtgcgtg
    17401 gcggtcacgc cgtgtgcgca cagcgcgacc ggcgcgccgt cacggcccgg ccagcgcgcg
    17461 acgcgaagcg tcccgccggc aaccggcacg tcgaacagct cgggcgcgcg catctacggc
    17521 agctccatgt gatccagctg cgcgcggatc accttcttgt cgatcttgcc gacgctggtc
    17581 ttagggatct cgtccacgat caccacgcca tcggggagcc accacttcgc gaccagcggc
    17641 gtcaggaact cccgcaccga ttcgagtgtc ggatgctcgt cgccggcggg aacgacgacg
    17701 gcgaccggcc gctcgtccca cttgcggctg cggatgccca cgaccgccgc gtcgcggatc
    17761 aaaggatggc cgagcagcgc gccttccagc tcgaccgagc tgatccactc gccgcccgac
    17821 ttcaccagat ccttggcgcg gtcgacgatc cggaaggttc cgtcgggctc catgaacgcc
    17881 atgtcgcccg tccgcagcca gccgtcgtgg aacttctcgc gcgagccctc cggatcgttg
    17941 tagtaggccg acgcgatcca ggggccgcgg cactccagct cgcccacggt cttgccgtcg
    18001 cgcgggcaca cgctgccgtc gggcgcgacg atgcgcgcct ccaggccggg aaagatcgtg
    18061 ccggtcttct cgcgctggtc cagcagcccg tcgcgatcca ggccgcgatg tccgcgccgc
    18121 ggcagcgaga tgcacgcgag cggaccggtc tcggtcatgc cccacatctg cacgatctcg
    18181 atgccctgct tgtcgtaggc gtcgatcagc ggccgtggca gtgcgctgcc gccgacgtag
    18241 atgccgcgca gcgacgaggc atcgacgtcc ttgcgctcga tcagcggcag caggtctttc
    18301 cagatcgtgg gcacacccgc ggtcagcgtg acacgctcct gctcgatgat gcggccgatg
    18361 ttggcggcgg atgagtccga gccgtggaag atcagctcgg cgccggcgaa cggagcggcg
    18421 taggggagcc cccagccgaa cgcgtggaac atcggcacga tcggaaagac gcggtcggtt
    18481 tcggtcagcg ccagcgttcc cgccatcgcg gtcgccatcg agtgcagcca catcccgcga
    18541 tgcgacgaca gcacgccctt gggcatgccg gtggtgccgg acgtgtagca cagcccgcag
    18601 ccggcatgct cgtcgatcgt gggcagcgaa tccggcgctg ccggggcggc cgcggacagc
    18661 agcgagtcgt agtcatgcgc gggggtcagg ccgtgcgcgt cgccgtcgcc catcacgacg
    18721 taggcgccga ccgtggccag cttcggcgcg acctcggcca gcgcgccgca cagctcggcg
    18781 tcgacgaaga tcacgcggtc ctcggcgtga ttggcgatgt aggcgatctg ctcgtcgtag
    18841 aggcgcacgt tgatcgtgtg cagcaccgcg ccgatcagcg gcaccgcgtg atacagcgcg
    18901 aaatgccgcg ccgaattgaa cgcgaacgtg ccgacccggt cgccggagcg cacgccgagg
    18961 ccgtgcagca ccgtgacgag cttgaggacc tcctcggaga gcgcgccgta ggtcgtgcgg
    19021 ccctcgggcg aaacgacctt gccgtcggcg tagacgtcgc gcatccggtc gaagatcgcc
    19081 cgcaaggtca ggggcgtgtg ctgcatcaga ccgggaatag tcatggcgcc ttcttcgctg
    19141 gggccttctt cgcgggggcc ttcttcgtgg cagcggccat cttgttcgcg ggtgccgctg
    19201 tcttcttcgc gcgcgcagcc ttcttcttcg tgggtgccgc ggtcttcttc gtgggcgcag
    19261 ccttcttgtc caagggcgcg gcggtcttct tcgtggacgc cgcggtcttc ttcgtggatg
    19321 ccgcggtctt cttcgcgtca actttcttga cgagtgccgt ggcgggaacc tccctggcag
    19381 gagctttctt cctcgcggcg ggagcgtcca gcagccgccc gatgcccaac gcctgcacca
    19441 tcgtctcgtg atcgatgccg gcgacgcgct cctgatatac gatcgtaccg cgctgcatga
    19501 tcaacgcgcg atgcgccagc cgcagcgcga acgccacgtg ctgctcgacc agcaggatcg
    19561 tcacgccgtc gcgattgacc tgcgttagcg cctcggccaa ctcgtccacg accaccggcg
    19621 cgaggcccgc ggtcggctcg tccaccagca tcagcttcgg ctggctcacc agcgcgcgcg
    19681 ccagcgccag catcgcctgc tcgccgcccg acagcgttcc cgcgggctgg tcgcgccgct
    19741 caccaagacg cgggaagcgc tcgtacatcg cagccaggtt gcgcgtatac cgttcccggt
    19801 cgcggcggcg cagcagatag ccgcccaagc gaatgttctc ctcgacgctc agctcgggaa
    19861 agacctgccg gccctgcgtc acgtgcgaga ttccgagccc gacgcgatcc tcgacaccca
    19921 ttgttgtcat cggccggccg aagaacgcga tttgaccgtc ccaggccggc acgatcccgg
    19981 cgatcgtctt catcgtcacc gacttgccgg ccccgttcag gccgaggatg cacacgatct
    20041 cgccttcggc gacggtgaag tccacgccgt gcagcaccgt gttcgagccg aagccggtcc
    20101 gaagcccgga cacgcgcagc gcctcggccg cgacgggctg ctcgagcgcg ctcatgcctt
    20161 cacccccgcc gatccgaggt aggcatccac gaccgtcggg tccgacacga tctcggccgg
    20221 cgtgccgtgc gcaagcactt tgccgaggtt cagcacgtac acctcgtcgc acacgtccag
    20281 aaccagcggg atgtgatgct cgatcagcag caccgtgcgg cctaggtcgt cacgcagctc
    20341 acgcagacgc tgcgcgagcg actcgatcac ggccggtgcc atgccggcgc tcggttcgtc
    20401 cagcatcatc atctcgggtg cggtggacag cgctgcggcg atctcgacga tgcgctgctg
    20461 tccgcccgac agatgcttga ccggcacgtc cgcgtagcgc tcgaagccca gcgatgcgat
    20521 cgcggcacgt gcgcgctcgt ccagctcgcg ttcctcgcgc gtgcggcgcg gcgtcgcggc
    20581 cagcgcatgc caggttccgt acgaggcgtg cgaatgctgc gccagcaata ggttctcgcg
    20641 cacgctcagg ttgcgggcca ggccgacgag ctggaaggtg cggccgatgc ccatcgcggc
    20701 gcgctgatgc ggcggcaggt cctgaatcga cgtgccgtcg tagatgacct ggccggcctg
    20761 cattttcacg aaacccgaga tcgcgttgaa cagcgtggtc ttgccggcgc cgttcgggcc
    20821 gatcagtccg acgatcctgc cacgcggcac cgtcagcgag gcgccgtcca ccgcgcgaag
    20881 ccccccgaac gcgacctgca cgtcgcggac ttccagcagc ggcggagcgt cggccgcgac
    20941 cgcagcgggc gcaagaccgg tgcgcggcag cgcgggcacg gcctgctcgt cggtgtcgcc
    21001 ggccagtcca gcccgcgcgg ccttcgcggc cttggcctct ttgccttcgc ggatcgcgcc
    21061 cgcgaacccg ccgggatgct tggcgagcgt atagatcagc aggaacgcgc cgaccagcag
    21121 atcccaatcc ttcaaggccg tcaggagccg cgggaagaag ccgaacagaa acgcggcgat
    21181 caccacaccg gcgcgactgc cgagcccgcc gatcaccacg aacgtcacca gcagcagcga
    21241 gaactgatag tcgaacacgt cggatgcgac ggtgccgacc agatgcccgt aggtcatgcc
    21301 cgcgatcccc gccagcgcac ccgagatcac gaacgccagc agcttgaagc gcgtcacgtc
    21361 gatgccgaac gattgcgcga cctgctcgtc ctcgcggatc gcgtggaacg cgcggcccag
    21421 acgcgaacgc accaggttga cgtccagcca ccagaacgcg accagcgtca ccaggatcaa
    21481 cgcaaggaag tcgccgtcga tcaggaagat gaaccgtccc gcgaccgggc gcggcagcat
    21541 caccgggccg ccgccttgcc aaggaagctg atacagcgtg ttctcggcga aaaagaccaa
    21601 gccgatcgtg gtgatcgcca gatacaggcc gcgcaatcgc agtgcgggca ggcccgcgac
    21661 gaaggccagc agggcgcccg caccggccgc gaacggcagc gtgatcaggt acgaccagcc
    21721 cagccgcccg gtcagcaggc cggacgtgta cgcgccgatg cccagcagcg ccgcatggcc
    21781 cagcgagatc tgtcccgcat agcccatcaa gatgttcagc gagatcgcca tcagtgcgaa
    21841 gcacgcgccg tagccgatca ccgacgcgcg gaacgacgac aggatgtcgg cgcgcgccag
    21901 cagcgggggt gcgatcacgc agaccgccag cacgatcagg atcgttcggg tgcgtttgga
    21961 aaccaccggc agcccggcgc cgcggagccg ctgcaccatg tcggtcatgt gcgcgcccgc
    22021 cccagcagac cctgcggccg cagcatcagc accagcagca tcgcggcgaa gatcgccatc
    22081 gtgcccacgc ccggcagcca ggtcacgtcg aggaaccatt gccgcacgac ctgatcgatc
    22141 agacccatca cgatgccgcc caggaacgcg cccgacggat tcgtcagacc accgaccagc
    22201 gcggcggcca gcgccggaac gaacaatccg ttgagcgtcc cggccgcgaa cccaccgatc
    22261 gacggctcga ccagaagcat cgcgatcgcc gacagcgcgc ccgccagcgt ccaggtgaac
    22321 gccgacacct tccacagccg cacgccgacc atctgcgtcg cgaccggatc ctgtgcggcc
    22381 gcgagcacac ccaacccgaa gtcggtgcga cgcaagaacc aggccaggcc gaagcccagc
    22441 gcggcgacga tgatcagcga caggatctgc gtcgggctga cgtgcacgcc caggatcttc
    22501 gggccgagcc cctcgatcgg atgcgacagg aaacgcggcg cggcggtcca aaccttgatc
    22561 tcgaacgcca tcaggaacgc gccgagcccg atcgttgcga ccgcgacggt caggcggggg
    22621 gcgtccatca tgcgcgacac gaccagccgc tcgaacgcga acgacaccag cgcggcggcg
    22681 gcgacgccgg ccagcgcgcc gaccatccag ggcatgcccg cctcttcgat caacgcccac
    22741 gccatgaata cacccagcgt gccggtttcg atcgcggcca ggttcagcac ccgcgatccg
    22801 cggtaaacaa ggacgatccc gagcgcgatg agcccgtaga tgccgccgac gacgatgccg
    22861 gagatcagtg cgctgcccat gtgatcccta gcccaggctt tccttgcacc acgtcgtggt
    22921 cttccaagct ccgttttggc cgcggtcgaa ggtctccagc gtcacgaact gatgcaggcc
    22981 aactcgcccg cccatcgact tcttgtgcgc gaagttggtc tcgcagttcg ggttgatgcg
    23041 cgtcttgagg ccggcgagga acgtgccggc gatccggttg cgtgtgcagg tgcggccgca
    23101 gtccagcagc atctggtgca ggaacttgtt gccgatccag acctgccaca ggatgtcgtt
    23161 gggcgtgacg ccgggacggt acttgcggta ggcggcttcg aagcgtttga tctcatcgtc
    23221 gtatccgtac tggctgaatg cgccgccgta tccgccggac gtgtaggcgc tccaggtgcc
    23281 cacgccttcg atggtcgggc ccagcgcgtt gttcttgccg atcagatcca gcgtcgtctg
    23341 gaacgggaac acgatccact tcggccggta gccttgttcg gctgcctggc ggatgatctg
    23401 tcccgcggcg agcgcgttct cccagatcca cacgaccttg acgtcggcgt tctgcagctc
    23461 gcggatctcc aggttgtagg ttccttgatt cttgatcgtc tcgaggtcct tggcgggcgt
    23521 gatgccgcgg cgcttcagct cggcgacgcc gctgtcgtgg ccgggcttcc agtactcgct
    23581 gttgcgcgag atgatcccca gcttcttgcc cttgtgtcgc gtcgcgatgt actcgccgaa
    23641 cgcgacgccg acctgctcga ccgtgggctg cgtcgagaac gagtgcaccg cccgttccat
    23701 gcccttggcg atcgccatgt ggtgcaggta gagcgtcttt tgccgctcgg cctcttctct
    23761 cgcgaacggg atctggtcga agccgatgcc gcccaggatg aagaacggct tcttgtccac
    23821 gcagcgcttg accaccgcgg ccgcttcggt gccgtcgtag cggtcgtctt cgtagctgat
    23881 ggcgacgcgg cggccgtgaa tcttgtcggc cgtgtcgttg acctgctgcc agtacacgct
    23941 cagatcggcc gcgctgatgt tgaacgctgc ggccagcacc agcgccgcgt gcccgcacag
    24001 cgtgatctgg ctgtcggtga tcccgatcgt gtcggccgag cccttgtaga tgttggccac
    24061 gtactgcttc ggcctgtccg tcgtgcggtt cgtcgggacc ggcccgcccg tcggggggag
    24121 gggcgtggtc gtgacgccgc ccgacgggat ggcggtcggc agcaccgtcg tgccgggcag
    24181 gggagtcccg ttctcggtcg ggccgccggc gaccggctct tcgtcgggat caacgatggt
    24241 gacctgcgga tcgttggcct tggcgccgta gtgccagtcg ggaagcgagc ccgagcatgc
    24301 gacggtgacc atgccgagcg cgaccagcgc cgcaaccgaa cgaaaacgtc tcacgaatac
    24361 cacctcgaag gaatcgccag gacgcagtcc tgattcgccg cggatggccc gatctcctgt
    24421 ctcatgtgta ctccacgtga agggtcgcgg ccgcgccgcg gccgatgaag atgtagttgg
    24481 tggcggtctg gttcgacgag aagcgaagct tgagctgcgt ggtgcccgtc ttgctgatcg
    24541 cgcccaggcc cgcggacgag aagttcgtcg acgaggtcgt gccgccggtc cacttcacga
    24601 tgttggaaac ggcactttgg gtcggcgcgg acgaccagtc gccggtctcg atcgtgcagg
    24661 cgccgaagca gccgttcttc acgtcgatca ccagcgtgtt gccggccggg ttggcccagg
    24721 gatccccgga cgtcgaattg agcgtcaccg tgatccacgc acgggtgatc gtggcggtgt
    24781 cgggcagcga ggcggtgttg aacgacagca cggtgcggtt gtagagcgcg tcgctgccgc
    24841 ggccgatcgc cagcccgtag gtggactcga gcgtgccgac cgctgcgctg ccgccgctcg
    24901 cgttcgcctt tacgtagccg tcgttggcgt cgtcgttgcc gaacgtgacg ctggtggtgc
    24961 cgccgccgcc gttgctgacc gtgacggtgg tgtcgtcgtc ggtgccgacg ttaccggccg
    25021 cgtccacggc cttggccatg atgcgatgcg atccgttcgg aaccgatccg gttgtccacg
    25081 tcagtgcgta cggcgaggag gtgtcggtcc caagcagcat gccgtccacg tagaactcga
    25141 ccttgaccac gcccacgttg tcggaggcgg tcgcggtgat gtccaccgcg ccgctcaccg
    25201 tcgcgccgtt ggcgggcgcg gtcacgttca ccgtcggggc ggtagtgtcg gcggcctgct
    25261 gcgggttgcc gatcttgatg tcgtcgagcc acgccttgcc ccaggcttcg atgcacacgt
    25321 tcgagttcgc cgcgacctcg aaggccagcg tcaccgtctg gttggcgaac gacgacagcg
    25381 acaggttcgc gcgctcggtc cagttcgaat cgacgtagtt cgcgtaggtg acggcctgct
    25441 ggtccacggt cgtggtgttg accttgacct tgaacgaggc ggtcgtcgag gtgttgatca
    25501 acgccttcag atccagcttg cgccagtacg acagcactgg gttgttcggc agcgtcaccg
    25561 agcgcgacag cgtctgggtc ttgttgccgg tcgcgcacga cacgccgctg gatgcgtacc
    25621 cgaacgcgct gttgccggct gcgttcgtgt ggttctcggt ggacgagacg tagccgccgg
    25681 cggtccagcc ggtggtgtcg tagtagtcgc cgttgccgtc gcggttgctg aaggtctcgt
    25741 ccagcaccgc gaccgtttgg ctgaccgtga ccgtcgtgtc gttgtcgatg ccgacgttgc
    25801 ccttcgcgtc gtaggccttg gccatcagcg cgtgcgagcc gttggatacg ctcgtggtgt
    25861 tccacggaat cgtccacggc cccgccgatc cggcgggtgt gcccgtgccg atcgacgcgc
    25921 cgtccacgaa aaactcgacc ttggtcacgc cgaagtcgtc cgaggcggac gcgctgatcg
    25981 tgaccgtgcc cgacaccgtc gagccgttgg tcggaaacgt gatgttgacc gtgggcggcg
    26041 tgacgtcctc gaccccgccg gtcaccgtaa ccgtcacctg ctgtgaggcc gtgctgtttc
    26101 cggccgcatc gtaggcacgc gcaagcagcg tgtgggtgcc gttcgcggcc gcgctggtat
    26161 cccaggtcgc gctccacggc gaggccgtgt cggtggcgag cagcgcgccg tccaccaaga
    26221 actcgacctt ggtcacgccc acgttgtcgc tggccgaggc gttgatcgtg atcacgccgc
    26281 tgaccgtgcc gcccgtcggc gaggtgatcg agaccgaggg cgcctgcgtg tcgctgttgt
    26341 ccaggccgaa gaagcgcgcc gtgaagtagg tcgagcagat gttgacgtcc aacatgaacg
    26401 cccctgcggt gccgcactgg tcggccgccg tgccgggatc gaccggcgtg ccgtggccca
    26461 tcgacgtgat catgtaagtc tcgacgacgc cgacgccgga tgcgttgcgg tagaccttgt
    26521 gcggatagcc cttgacggtg tcttcaacgt cggcgatctg gtcggtgccg tgcacgttcg
    26581 tccactgctc catcgactcg gtcgcgttga ccgggtgcac cgtcgtgtcg ctcgttccgt
    26641 gccagatcga caccttcggg tacgggccgg aatacgccga gtaggcgccg cgtaccaggt
    26701 cgccccactg cgccggcgtc ttgttcacgc ccgggttcat gcacgaaaag ccctgcgaca
    26761 gtcccgtcgc gcacttgtac ggcacgcccg ccacgatcga gccgcccgcg aacacgtccg
    26821 gataggttgc cagcatcacc gcggccatcg cgccgcccga ggacaggccc gatacgaaga
    26881 tgcgtgccgc gtccgagccg tggtcggtct tcatcttgtc ggtcatctgc ttgatcgaca
    26941 gcgcctcgcc cgcgccgcgc gcgtagtcgc cggactcgaa ccagttgaag cacgacgacg
    27001 agttgttgga cgactgctgc tgcgcgaaga ccagcgcgaa ctgccacgcg tccgcgtact
    27061 tcacccagcc cgactcgtcg tcgtaggagg cggcggtctg tgtgcagccg tgcagcagca
    27121 caaccagcgg cttgttcgac gacaggccgg tgggcacgta cttgaacatc ttgaggttgc
    27181 cggggttggt gccgaagctc gtgacctcga ccagcgccgc ggccggagcg gtgcccggcg
    27241 gcagcagcgt gaacaggatc gcgagcgcgg ccaccgccgc cgttcgcatg cgtcgcctgc
    27301 gggaaatgcg aaactcgacg ggtgccctca ccttgacccc cagtgctccc caaggtccgc
    27361 cggccggtcg gggaagggct ggcgccggcg gtgttccaga agtaaaatat gaatcacctg
    27421 tcagttttac tcgccggtaa tgtaacgctc gccggaaggg agagtcaagg gtggcattgg
    27481 gccgggaagc ggcaaaggcg gtcgcgcgga aagccgcgcc ggccgggcgc gccgtccaaa
    27541 agcccgccgc ggccgccgag cccgcccgcc ggctgaccaa gggcgagcgg acccgcgccc
    27601 gcctggtggc ggccgccgag caagttttcg agcagggcag ctacgacgag gccagcatcg
    27661 tcgagatcac ccgccaggcc ggggtcagcc aagggacctt ctatctgtac ttcccgtcca
    27721 agctggagat cttccgcgag gtcgtccggg atctgagcca tcagctgcgc agccacatcg
    27781 cccagacggt ggagggcgcg gccacccgag ccgaagtcga acgccaaggc ttcgaagcgt
    27841 tcttcgcctt cgtgcagcag caccccggcc tgtatcggat catccgccaa gccgagtttg
    27901 tggaccgcga tatctatcgg taccactacg agcgtctcgc cgaggggtat accagaggcc
    27961 tgcgcgccgc cgtcaaagcc ggggagttcc gcgacgtcga cgtcgaagtc ctcgcctacg
    28021 ccctgatggg aatgggcgag ctgatcggag cccgctggct gctctgggaa gacgagcccg
    28081 gcggcaaggt tccggccaaa gtcgtggacg cactcgtggg gatcgtgctc gaaggcgtca
    28141 agaaacccaa ctgatctagt cagataatgc ggcgcgtgca ttcaataatt tggctgcgca
    28201 ccagggaccg gctcggcagc ccgatgccgt ccttgccgca ttcgccgaac cggacgcgct
    28261 tgtcgtgacc caagacgagg cgacctgcga gcccttgccg cgccacgcgt gcgcatcgcc
    28321 gttgtctagc agaatccttc gctcccagag gttggcggcg gacgggtgga caatggcacc
    28381 cacgaggcgt cgatacgggg gagtcccatg ctctacaaaa tcttgctgtt cgtgcacgtg
    28441 accggcgtgg tgttctgggt cggggccggc ggagcgttcc agatcatggc cgagcgggtc
    28501 gcgaagatgg attcttcgcg ggtcaaggcg ttcattcaaa cgggctcgtt ggtgccgtcg
    28561 gcctacttcg ggatcctgac cgcggtcgtt ctggtctcgg gcgtgtggat ggtggtcgat
    28621 tcaggcatcg ggttcgagca gccgttcgtg gtcgcgggcg tcgtgggcct gatcgcatcg
    28681 ggtgcgatcg gcggagcagt gatgggcaag acctccgcct cgctctacac gctggtcgca
    28741 tcaccgaact tcgacgagaa ggtgatgaac gcgggcgtgg ccaagatgcg cacgttcggc
    28801 cgcctggatc tggcgatcat gctgttcgtc atctacatga tgacggtcaa gcccgggacc
    28861 taagccgcgg gccgcacgat ttcgaactgg gccatcatcg cggtgtcttc gtgttcgccg
    28921 atgtgacacc ccctttgcag ggcttgtttg aggggcgttg cccccgctcg ttttccttgc
    28981 ttgttggact cgctgtcggt gtcgtcgggt tgaatgagga acctttcggg tactccgatc
    29041 cataaggcgg acggcacccc gtcgcgttcc gcatttcccc gcacgcaagg agaaaccatg
    29101 tccgagatca aggagtcggt tgaagtcgac gttccggtga acgttgctta caaccaatgg
    29161 acgcaattcg aagagtttcc gcagttcatg gaaggcgtcg aagaagtcag gcagctctcc
    29221 gacgaccggc tgcactggaa ggccgagatc gcgggtaccg agcgtgaatg ggacgctcgc
    29281 atcaccgagc aggtgcctga ccagcggatc gcgtgggtca gcgaaggcgg cgaagccaac
    29341 gcgggcgtcg tgacgttcca caagatcgac gacaacacca cgcgcgtcat gctgcagctc
    29401 gactacgaac cgcacggcat gctcgagaag gccggcgaca tgatgggcat ggtcaagcac
    29461 cgtgcctccg gcgacctgaa gcgcttcaag gacttcatcg aaggacgcgg ccgcgaaacc
    29521 ggcgcgtggc gcggcaacat ctcgtagcct gccgctcgct tcgatgatgc ggctcggcgc
    29581 taggcgtcga agccgattgc ggcgatgggc gtggccagtg acatgtcggg gcggccggcg
    29641 gccatcggca tgaactcgcc catcgccgcc atcatcgcgg gcgtctgccg gtgcgcggcg
    29701 acggcttgct cgtcggccat cacggcgaag aaccagaacg tatccccttc gccgcggtga
    29761 tacgagtaca cctcgactcc cggctcgccc tttgcggccg cgaccatctt cgccagcacc
    29821 ggctcggcct cgtccgcctt gcccggcagc accgtgatct ttcccaccat cgaaaccttg
    29881 ctcatcgcgt cctcctcgga gtcgttcggc gcgcccacgt tacacggcta gccgagcagc
    29941 agacgcttcg cggcggtgac cgcatcgggt gagccgccga cggtgaatcc gccgggctcg
    30001 aacgagaagg tgaaaaacgg acagcaccgc gattcgaggt ccgcgaggcg gcgagcttcc
    30061 gcatcgtctc ccggctcgac gcggatgtga agcccgtccg gtcgcatctc ggcgccgcgt
    30121 gatcgcgcga cgagcgcgcg ccactcggcc gcgcgctcct tctggccgct cgcgtccagc
    30181 gagcaggcga tcagcggcag ctctttcatc ggcatcgagc ctatcccgcc aagcgggggc
    30241 ggcgcgcgtg aataccggcg aggccgaagg ccgcggcgcc ggcgatctcg acgatcacgg
    30301 cgaaccacga cagcgcgttc actcccacgg ctgaggcgcc cacgaaggcg tcgctgaacg
    30361 cgctccaccg cggaagcaga tgcacggccg cgatgccgat cgccaccggc agcccggtcc
    30421 acgcggccgc ggtggagccg cgccggtcgc gcaccaagac cagcgcgatc gcgatgccgc
    30481 ccgctacggt cgagatcgcg ccgatcatct tcacctgcgg cgtgatcacg tcggcgccgc
    30541 ggcgaagatg atcggcggtg tgcagcgcga gccccgcggc gtagagccag gcggctgagc
    30601 gaagacggcc gttcatcgcg ggcctccggc gcgggcggcg gacgccgcga cgcacgcgag
    30661 ccgcgccgcc tcgatcgccg aggcgaaccc gacctcggac agcaggcccg gcgccgccac
    30721 ctgatcgccc gcgacgaaga tgcccccgcc acggtcgatc gcgggccggt cctgccaggt
    30781 cgttccgggc aggtcgagcg caccggtctt gccgtcgctg atatgcgatc gagaccagac
    30841 ggctcgctcg cgccagcccg gaaacgacgc gtcgagcatc gcctccagat gcgcgatgcc
    30901 gtcctcaagg ggtatgcccg gctcgaggcc ggcttgggcc tgcagcagcg acacacccgg
    30961 gggagcgagg ctcgggtcgg gcttcgtgta gcgctcgatc catccgggcc ggtccaggtt
    31021 ccacaccagg aacggatcgc cgcgccggga ctcgagcgcg acgtcgagca gcgcgcaccg
    31081 cgccgactcc caccgcaggg tctcgtcgcg cagcagatcg caggcggcgt gcaggcgcgt
    31141 ggccacgatg gagacgggct cgacctgatc gacgcgcacg ccggtctcga tgcgcacgcc
    31201 gatcgatgtc gcatgacgcg cgagcacgtc gatcagcgaa ccccagccgc cgtgcacgta
    31261 cttgacgtgc cgcgccgata cggcgacgcg gcgaagctgc gtccaggcga acgcggccga
    31321 aagccgtccg ggatcgtgat cgaaggtgac cacgcctgcc gccgcgcaca gcatcgcggc
    31381 cgtgcgctcg tcggtcttcg ccgacagcca ggtccgcaga tccacgtcgg ccggaggctc
    31441 ggccctgccg agtcgcgtca tcgtcgtcac tgccgcggcc ggcagcatct tgcgcaccac
    31501 gccgccggcg atgaagcgaa agccgctgag cgcggggccg gagtgcggcg gggtcaggtt
    31561 ccgctccgcc agccagcgcc acagcgggcc gtcgccgtag atcacgtgcg ggccgaagtt
    31621 tgcgcgatag ggaggggcgg tcgtgcgcgc tcgtccgccg aggcggcgct gcgcttccag
    31681 cagtcggacc gaggcgccct tctcggccgc ggcgatggcc gccaccagcc ccgtgagccc
    31741 tccgccgacg atcgtaatct cgctcatcct cgcctcccgt gcccgtcctc tgctaacata
    31801 catacatgac tgtacgttta tcgcagcgtc cgggcaagtc gcgggccacg cagacggagc
    31861 ggcgcgcacg cagccgcacc gcgctgctcg aggccgcagc gcgcggcctg tcccgctacg
    31921 gctacgggaa cctggtcctg gaagaggtcg cccgcgaagc cggatacacg cgcggcgcgc
    31981 tctaccacca gttcgccgac aaggaggacc tggcgatggc cgtgcttcgc tgggtggacg
    32041 agacctggcg cgacgaggtc ggcaagcaag tcgaaggcga aaccgatccg ctggcgattc
    32101 tgaccacgct cgcacgcggc cacgcgatct tttgtcgccg cgacatcgcc cgtgtcgcga
    32161 acgcgctgcg catggagttc agtggccgcg accatcccgt cggccgcgag gtcgagcgcg
    32221 tgtcgcagac gctgatcaag cgcgtgatga agctgatcga cgcaggccgc cggcaagggt
    32281 cgatcccacc cggtccgccg tcacgcgccg tcgcgcacgc gttggtcggc gcgctggaag
    32341 gcgccgtgat cgaactggcc ggccatgcgc cccacgacga gaagcttgcg gtgcgcgccg
    32401 cgctcggcgt gctcggcctg aatccggccg tccacgaaac cgccatgaaa ggacggccgc
    32461 gatgagtgac cttcgaaccg accacgcgct gttccgcctg cgcgatgcgt ttccgcgcgc
    32521 gcgacgcgac gacacgctgc cacccggtca gcgcgcgatc tcgacgtttc cgcggtacgg
    32581 catcgccaga cctgacccgc ccgttcccgc gcatccgatg atccagatca tgggcgcaca
    32641 ggacgcggtg ctggaactcg acgcggacgc gctgaatggc ttccctcgcg tccgcgtcgc
    32701 cgccgacttt cattgcgtcg ccggatggac cgcgcgcgac cttcgctggg aaggcgtgcg
    32761 ctttcgtgac gtctacgacg cgctgagcga gcgcgcgccc gtcacgcaca tcttggtggt
    32821 tgggcgcgac ggctggcgcg ccacactgct gttggaagac gcgctgcgcg acgacgtgct
    32881 gatcgcggat cgcctggacg gcgagccgct gccgctggac cacggcggcc cgttccgtct
    32941 ggtcagcccg gcacagtacg gctacaagaa cgtcaagcat ctggtccgca tcgaactgca
    33001 cacgagcgag ccaaacgacg tggtcggcgg atacttcgac cgcttgctgt tccgcacgct
    33061 gttggccaag catccgcgcg cacgggtcgc acacgaagag cggcacaggt ccatgccgag
    33121 cctgacgacg cggcgtatct acaccaagct tcacccgatt acgtacctgc tcggctacat
    33181 cggcgcgcgc cgcaacccgc gctgacatac gctgcaggca tgagcgagat catcctgttc
    33241 caccacgcac aaggcctgac cgatggcgta cgcgccttcg ccgatgagct tcgcgccgcc
    33301 ggtcacaccg tgcacacacc cgacgtctac gacggcaaaa ccttcgacac gctcgaagac
    33361 ggcatcgcct acgcgaagga gatcggcttc ggcaccgcgc tcgaacgcgg ggtcgccgcc
    33421 gccgaaggcc tgtccgccga aatggtctac gccgggttct cgctgggcgc gatgccggcg
    33481 gtcaaactgg cgatcacgcg gcccggcgtg aaaggtgtca tcttgatgca ctcggtcgcg
    33541 cccccgtccg agccgcagac atgggtggcg caggcgtacc tgatcacgtt cggaacagtc
    33601 ggcgtcttcg gcctatatct gttcctgttg agccggtgga cggcaagcgc ggtctcctac
    33661 gagttcgtgc tcgccccgct ggc
//
LOCUS       DATLFO010000169         8868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_3490, whole genome shotgun sequence.
ACCESSION   DATLFO010000169 DATLFO010000000
VERSION     DATLFO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3490"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VM841_06810"
     CDS             <1..505
                     /locus_tag="VM841_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89929.1"
                     /translation="GDCAGSIGGRNWSSFTGLLQGMESGLQQAREQAMREITRLRERV
                     EPELEAARQEIADETATLRRDLVSSEETFTGALRAFANRLETLQSQLSEAGARMAAER
                     ASAAAQLQSMNGRIDTIEERITEGLARLSEGILRVAENVGSATTLQRRISALESTITE
                     MQSRQQG"
     gene            complement(514..912)
                     /locus_tag="VM841_06815"
     CDS             complement(514..912)
                     /locus_tag="VM841_06815"
                     /inference="COORDINATES: protein motif:HMM:NF020512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HVL89930.1"
                     /translation="MPKVGDRVVVEGSKVGGGRREGTLRKVVGSLIQVSWEDGSETLF
                     APGAGTVQFLPAGKTNGKTAKASKPASTRDRGKPASQGASKAPARAGGKSLAKKTVAK
                     AASKAGAKAGAKAGAKAPSKGGAKKAGKKK"
     gene            1263..2806
                     /locus_tag="VM841_06820"
     rRNA            1263..2806
                     /locus_tag="VM841_06820"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(3023..4234)
                     /locus_tag="VM841_06825"
     CDS             complement(3023..4234)
                     /locus_tag="VM841_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVL89931.1"
                     /translation="MRRLARMRRLWAASILPIVAPLIFAVASMADIGYLGWRCLESPQ
                     REGFGSEVYGIGTRTQAALQYRLFVNRFSAECRPDVQGLVSFRGESEDVVVGSMIQRE
                     RDPLGRPYTFYALDRPLTTAEKERIETDADGRLLRISPVLQIPLWIEGIALAYNPPCP
                     VTGLKLSSAALTAVFSGVAERWNDPLLVTDNPQLALCTSAIRVAVRSDDSAQTTVFKD
                     YLSKRSASWKAYMTPDRNRDWPATLLDPCRGRGDQGMVGCIAGLPGSIGYVGFAEAFR
                     NGLPVAAVENRAGFVGPSLDECTRAAESPTGGYPARADLDWSATSLTDPPSGYPICNL
                     VFALAFEKMSPAYGGSITVAEVRTVRDFVWTAVRADTQRRLTRYGVAPLPEFVRSLIA
                     EPGAESIGYFE"
     gene            complement(4231..8433)
                     /locus_tag="VM841_06830"
     CDS             complement(4231..8433)
                     /locus_tag="VM841_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89932.1"
                     /translation="MTRIRRHSVSRPLLGGLIALSAIVTLLPAEAPARTRAGRYDRSG
                     INGSEQAAVRPVTLGVRAGLVGAVPGSASHEAWAVGRSTAKVPGWAGDHGGGQTVFLR
                     YTRSTGWRLLGPPRSAGGTPINPTITALSLAPGGAGWAVGGGGTMLRLSGGTWIEAAR
                     VTDASLSSVSITSAGGRTIGFAIGGGPTVLRLDGDRWLPDPLGIAADDIDLRSVSVVS
                     AQQAWIAGRVGRAALIARRGDTGWTRVMTGREMFDGEATRVVGTTIVATTPANTVAAS
                     AAGAWIGGAIVPVDAATAQASAAGDPTRPFALFVGRDGRITSFCPDRYAMGSDGRAQT
                     SALCDEPFPLAAFGISAMHVFPGAGRGEVFAGGLGLFHYRDGAWFREPDPVSYVSSMG
                     FVNRTEGWFAGTGGTTAATGSLSTLHTLAHWTRTPATPRMARHPQPMTDSFTGVSHPV
                     EAVAISPDGSGEALAVGRAGAMLRHAPRVGWDTFTPVTAQSLHAVAYRSAREAWAVGG
                     RGFLASYRDGIWRQYGTDAVSMTTASLFGVAFDGEARGYAVGSNGTILTYDGRRWRHD
                     AASRRVTDADLYAIARAGREFVAVGADGTVLETRGRTWRRAPGVGPLVDRGGLLPALY
                     TVAGLPDGTAVAGGELSTLIRRDRATGAWRIERQGSRVPPEGTILALAARRAGGGLDL
                     IVSLAQDPLKYAGEIPAATKGVVLYGTSDGWRDLDGRSRETIFDSFDASATRDPVLSV
                     AFDGSGAWAVGGIGPGNDDGQGHIQGYPTSSIYRIEPSGDPRPTGFRASPRLDASSNL
                     ISFAFFGESSCGRGLCSAAVGTGTMADVISSQIRDEINGMAALPGGPTFAVFGGNMRR
                     SGIPEELGQFKHYAKGFKVPLFATLGGNDLFTGIEAPGVLPEQVSPDHSFYIDTFKDR
                     PAPWGEAKPLPGFIPVLDDPSVSGARTHYAFDYAPGGERRLRVIMLDDSAPARLSTAA
                     TQNPPRDQNTWLTDALRDARDRNLPAIIVMNRPARNPLDLSNPETSYDEALPVQAAAA
                     NVGASAVLTSYFKENAVLMVSVGDSGTVPVYVFGGGGAPLETKPNDSPPVAPEPAQGY
                     YHSWQLVTVDLSRRTSLGQADVSVQSFPVVDGLALHAIDGVNVPGGNTLRFTSSGRTP
                     EGGGPADPLQSRAAVIPMDFRTRGVCAPDASRSFRPRCRSTGPVGPAFRYESEDPSVG
                     YFVTPSAASDRTPALDPTTGLPIPDPSSGLFCAVGGGTTYVSVISGFHRARMQVTVTS
                     GEGPCTKKRIFVPEVVPPEPPVFVSQPEAAVPVKAFGFQFAPEQQTTVVLPPPPITVA
                     APAPPGAGGYAKKEEHESAPEQATDDHAFTALPRRARAERDAQQAWTILGGAIMLSFW
                     SAAAAAAVYRRRSMARPEESRR"
     gene            8593..>8868
                     /locus_tag="VM841_06835"
     CDS             8593..>8868
                     /locus_tag="VM841_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89933.1"
                     /translation="MRRTAVTLIACFALAACGGGASDPAGHGRGPDGSPDSLASPGAS
                     AGTGAPSGPGVPAAPGTVTLPPEATARPSDFPVPDFVKAEVPEDEAWG"
BASE COUNT         1429 a         2945 c         3180 g         1314 t
ORIGIN      
        1 cggcgactgc gcgggctcga tcggagggcg gaactggtcg tcgttcaccg gcctgctcca
       61 aggcatggaa tccgggctgc aacaggcccg cgagcaagcg atgcgggaga tcacccggct
      121 ccgcgagcgg gtcgagcccg agctcgaggc cgcccggcaa gagatcgcgg acgagaccgc
      181 gacgttgcgc cgcgacctgg tctcgagcga agagaccttc accggcgcgc tgcgcgcctt
      241 cgcgaaccgg ctcgaaaccc ttcagtccca gctgtcggag gccggggccc gaatggccgc
      301 cgagcgggcg tccgccgcgg cccagctcca aagcatgaac ggccgtatcg acacgatcga
      361 ggagcgcatc accgagggcc tggcgcgcct gtccgaaggg attctgcggg tggccgagaa
      421 cgtgggctcg gccacgactc tgcagcgacg catctcggcg ctcgagtcga cgatcaccga
      481 gatgcagtcg cggcagcagg gctagaagcg gccctatttc ttcttgccgg ccttcttggc
      541 cccgcccttc gagggagcct tcgccccggc cttcgccccg gccttcgccc cggccttcga
      601 cgcagccttg gccacggtct tcttcgcgag cgacttcccg ccggcgcggg ccggggcctt
      661 gctcgctccc tgggaagcgg gcttgcctcg atccctggtg gacgccggct tgctcgcctt
      721 ggcggttttg ccgttggtct tgccggccgg aaggaactgg acggtgccgg cgccgggggc
      781 gaacagggtt tcggacccgt cctcccaact gacctggatc agggatccca cgaccttgcg
      841 cagggttcct tcgcggcggc ccccgccgac cttcgagcct tccacgacga cccggtctcc
      901 taccttcggc atctggtgat cctcccctat gaggtacgat acggacgttt tcccggttca
      961 cggattcggg tttgccaatc cgcgaccgcg cgctatacta gcgctccgct gtccactggg
     1021 gacggcggaa acgaccaaac tcctagcccg agatttgggc tggcgtttgg tcgctgtctg
     1081 cgccttgaaa accgcatagc gaggacacgc gattcgatcc cggaaggaag atcttcggat
     1141 cgaccaaccg gggggaacgc aagtgagagc gggcaccctt ttgtaacgcc ttgttagaga
     1201 tcatcatcgg ccgagtgaag ttcgacttcg gtcgagcgga aatcggtcgg tgagcaaaac
     1261 ctctaatgga gggtttgatc ctggctcagg atgaacgctg gcggcgtgct taatacatgc
     1321 aagtcgagcg atcggacctt gggtggtaac acccgaggtt acggagcggc gaacgggtga
     1381 gtaacacgtg accaacctgc ccacgagacg gggataacgt tgggaaacca gcgctaatac
     1441 ccgatgaccc tgcgaggtgg catcaccacg caggcaaatg gattccgctc gtggaggggg
     1501 tcgcggccta tcagcttgtt ggtggggtaa cggcctacca aggcgacgac gggtagctgg
     1561 cgtgagagcg ctaacagcca caccgggact gagacacggc ccggactcct acgggaggca
     1621 gcagtaggga attttggaca atgggcgaaa gcctgatcca gcgacgccgc gtgagggatg
     1681 aaggccttcg ggttgtaaac ctctgttgta agggaagaag ccaccgcaag gatggtgacg
     1741 gtaccttacg aggaagcccc ggctaactac gtgccagcag ccgcggtaag acgtaggggg
     1801 caagcgttat ccggattcac tgggcgtaaa gggcgcgtag gcggctacgt aagtccggcg
     1861 tgaaatccca gggctcaacc ctggaactgc gtcggatact gcgttggcta gaggatggta
     1921 gaggttcggg gaactagcgg tgtagcggtg aaatgcgcag atatcgctag gaacgccggt
     1981 ggcgaaggcg ccggactggg ccactcctga cgctgaggcg cgaaagctag gggagcgaac
     2041 cggattagat acccgggtag tcctagccgt aaacggtggg tgctaggtgt ggggggcgta
     2101 tgtccttccg tgccgcagct aacgcattaa gcaccccgcc tggggagtac ggccgcaagg
     2161 ctaaaactca aaggaattga cgggggcccg cacaagcggc ggagcgcgtg gcttaattcg
     2221 atgcaaagcg aagaacctta cctgggcttg acatgcacgt agtaggaacc agaaatggaa
     2281 ccggcccgta aagtcgggtg cgtgcacagg tgctgcatgg ctgtcgtcag ctcgtgccgt
     2341 gaggtgttgg gttaagtccc gcaacgagcg caacccccgc cctttgttgc cagcggataa
     2401 tgccgggcac tcgaagggga ctgccggcga gaagccggag gaaggagggg atgacgtcaa
     2461 gtcctcatgg cccttacgtc cagggctgca cacgcgctac aatggtcggt acaatcggtt
     2521 gccaactcgc aagagtacag ccaatccgaa aaagccggtc gtagttcgga ttggggtctg
     2581 caactcgacc ccatgaagct ggagtcgcta gtaatcgccg atcagcatgc ggcggtgaat
     2641 acgttcccgg gccttgtaca caccgcccgt cacgtcctga aagttggcaa cacatgaagc
     2701 cggtggccca accgcaagga gggagccgtc gagtgtgggg ttggcgattg ggacgaagtc
     2761 gtaacaaggt agccgtaccg gaaggtgcgg ctggatcacc tcctttctaa ggagataacg
     2821 tcactcctta aatctgggtt cacccgcttt cacgtcctcg ctatgtggct tccgaggcac
     2881 agactttgaa aggcgtcctt cgggacgcct ttctgcattt ccgggcacca tccggcactg
     2941 cccgcgtcac cgcgacactc gtcaattttc ttgacgagtg tcgccacgga ccaacgtccc
     3001 ccgtggccgg gtgggggccc tgctactcga agtagccgat cgactcggct ccgggctcgg
     3061 cgatgaggga gcggacgaac tcggggagcg gggcgacgcc gtagcgggtg agccgccgct
     3121 gcgtgtcggc gcgcaccgcg gtccacacga agtcccggac ggtgcgaacc tcggcgacgg
     3181 tgatcgagcc gccgtaggcg gggctcatct tctcgaacgc gagcgcgaac accagattgc
     3241 agatcggata gcccgagggg ggatcggtga gcgaggtcgc gctccagtcc aggtccgcgc
     3301 gcgccggata gccgccggtg ggcgactcgg ccgcccgcgt gcactcgtcg agggagggcc
     3361 cgacgaagcc ggcgcgattc tcgacggccg cgacgggcag gccgttgcgg aacgcctccg
     3421 cgaagccgac gtagccgatc gagcccggga gcccggcgat gcatccgacc atgccttgat
     3481 cgccgcggcc gcggcacggg tcgagcagcg tggcgggcca gtcccggttg cggtcgggcg
     3541 tcatgtacgc cttccacgac gccgagcgct tcgacaggta atccttgaac acggtggtct
     3601 gcgcgctgtc gtccgagcgc accgcgacac ggatcgcgga ggtgcacagc gcgagctgcg
     3661 gattgtcggt cacgagcagc ggatcgttcc agcgttcggc cacgccggag aacacagcgg
     3721 tcagcgccgc cgacgacagc ttcaggccgg tcaccggaca cggcgggttg taggcgaggg
     3781 cgattccttc gatccacagc gggatctgaa gcaccgggga gatgcgaagc agccgcccgt
     3841 cggcgtcggt ttcgatgcgt tccttctcgg cggtggtcag cggccgatcg agcgcgtaga
     3901 aggtgtaggg acggccgagc ggatcccgtt cgcgctggat catcgatccg acgacgacgt
     3961 cctcgctctc gccgcggaac gacaccaggc cctggacgtc gggccggcac tcggccgaga
     4021 agcggttcac gaacagccgg tactgcaggg ccgcctgggt tcgggtgccg atcccgtaga
     4081 cctcgctgcc gaagccctcg cgttgcgggg attcgaggca tcgccaaccg agatagccga
     4141 tgtcggccat cgaggcgacc gcgaagatca gcggcgcgac gatcggcagg atcgaggccg
     4201 cccagagccg tcgcatgcgc gccaggcgcc tcatcggcgc gactcctcgg gacgggccat
     4261 cgaccggcgg cgatacacgg ccgcggccgc ggccgccgac cagaacgaca gcatgatcgc
     4321 gccaccgaga atcgtccacg cctgctgcgc gtcgcgctcg gcgcgggcgc gacgcggaag
     4381 cgcggtgaac gcgtggtcgt cggtggcctg ctcgggcgcg gactcgtgct cctccttctt
     4441 cgcgtatccg cccgcgcccg gcggcgcggg ggcggccacc gtgatcggcg gcggcggaag
     4501 cacgaccgtg gtctgctgct cgggagcgaa ctggaagccg aaggccttga ccgggaccgc
     4561 cgcctccggt tgcgagacga ataccggggg ctcgggcggc acgacctcgg gtacgaagat
     4621 ccgtttcttc gtgcacggcc cttcgccgct ggtcacggtc acctgcatgc gcgcgcgatg
     4681 gaagcccgag atcacgctca cgtacgtcgt cccgccgccg acggcgcaga acaggcccga
     4741 cgacggatcg gggatcggca gcccggtcgt cggatcgagc gcgggcgtgc ggtcgctcgc
     4801 cgcgctcggc gtgacgaagt agccgaccga cggatcctcg ctttcgtaac ggaacgccgg
     4861 tccgaccggc cccgtcgagc ggcatcgggg ccggaacgag cgcgatgcgt ccggcgcgca
     4921 cacgccgcgg gtgcgaaagt ccatcgggat tacggccgca cgcgactgca gcgggtccgc
     4981 ggggccgccg ccctcgggcg tgcggcccga cgacgtgaac cgaagcgtgt tgccgccggg
     5041 cacgttcacg ccgtcgatcg cgtgcagcgc gagcccgtcc acgaccggaa acgactggac
     5101 cgacacatcg gcctggccga gcgatgttcg ccgcgacagg tccacggtga cgagctgcca
     5161 ggagtggtag tagccctggg cgggctcggg cgcgaccggc ggcgagtcgt tcggcttggt
     5221 ctcgagcggc gcgccgccgc cgccgaagac gtagaccggg acggtgcccg agtccccgac
     5281 cgagaccatc agcaccgcgt tctccttgaa gtacgaggtg agcacggcgc tcgcgccgac
     5341 gttggcggcg gcggcctgaa ccggcagcgc ctcgtcgtag ctcgtctcgg ggttgctcag
     5401 atccaacggg ttcctcgccg gccggttcat cacgatgatg gccggcaggt tgcgatcgcg
     5461 cgcgtcgcgg agcgcgtcgg tgagccaggt gttctggtcg cgcggcgggt tctgcgttgc
     5521 cgcggtcgaa agccgcgcgg gcgcgctgtc gtcgagcatg atgacccgca gcctgcgctc
     5581 gcccccgggc gcgtagtcga acgcgtagtg cgtgcgcgcg cccgacaccg atgggtcgtc
     5641 gagcaccggg atgaagccgg ggagcggctt ggcctcgccc cacggcgcgg gccggtcttt
     5701 gaacgtgtcg atgtagaacg aatggtccgg cgagacctgc tccggcagga cgccgggcgc
     5761 ttcgatcccg gtgaacaggt cgttgccgcc cagcgtcgcg aacagcggga ccttgaagcc
     5821 tttcgcgtag tgcttgaact ggcccagctc ctcggggatg ccggagcggc gcatgttgcc
     5881 gccgaagact gcgaaggtgg gtccgccggg aagcgcggcc atgccgttga tctcgtcgcg
     5941 gatctgcgag gagatcacgt ccgccatcgt gcccgtcccc acggccgcgc tgcacaggcc
     6001 gcggccgcac gaggattcgc cgaagaacgc gaagctgatc aggttcgagc tggcgtcgag
     6061 ccgcggcgat gcgcgaaagc ccgtgggccg gggatcgccc gagggctcga tgcgatagat
     6121 cgaggaggtc gggtagccct ggatgtggcc ttgcccgtcg tcgttgccgg gaccgatgcc
     6181 gccgaccgcc cacgcgccgc tcccgtcgaa ggcgacggac agcaccgggt cgcgggtcgc
     6241 ggacgcgtcg aaggagtcga agatggtctc gcgggagcgg ccgtcgaggt cgcgccagcc
     6301 gtcgctggtc ccgtacagca ctacgccctt ggtcgccgcc gggatctcgc cggcgtactt
     6361 gagcggatcc tgcgccaggc tcacgatcag gtccagcccg ccgccggcgc gccgcgccgc
     6421 cagcgcgagg atggtccctt ccggcgggac gcggctgccc tgccgttcga tgcgccacgc
     6481 gccggtcgcc ctgtcgcggc ggatcagcgt ggacagctca cccccggcca cggcggtgcc
     6541 gtcgggcagg ccggcgaccg tgtagagcgc gggcagcaga ccgccgcgat ccaccagcgg
     6601 tccgacgccc ggcgcgcggc gccaggtccg gccgcgtgtc tcgagcaccg tgccgtcggc
     6661 gccgaccgcg acgaactcgc ggcccgcgcg cgcgatcgca tacaggtcgg catcggtgac
     6721 gcggcggctc gccgcgtcgt gacgccaccg gcgcccgtcg taggtcagga tggttccgtt
     6781 cgatccgacg gcgtagccgc gggcctcgcc gtcgaaggcg acgccgaaca ggctcgcggt
     6841 cgtcatggag acggcgtcgg tgccgtactg ccgccagatc ccgtcgcggt aggacgcgag
     6901 gaacccgcgg ccaccgacgg cccaggcctc gcgtgcgctg cggtaggcga cggcgtgcag
     6961 cgactgcgcg gtgacggggg tgaacgtgtc ccagccgacc ctgggcgcgt ggcgcagcat
     7021 cgcaccggca cggccgaccg cgagcgcctc gccggatccg tcgggcgaga tcgcgacggc
     7081 ctcgaccgga tgcgagactc ccgtgaacga gtcggtcatc ggctgcggat gccgcgccat
     7141 gcgcggcgtt gcgggcgttc gcgtccagtg cgcgagcgta tgcagcgtcg agagcgatcc
     7201 ggtcgcagcg gtcgtgccgc cggtgccggc gaaccatccc tcggtgcggt tcacgaaacc
     7261 catcgacgac acgtacgaga ccgggtcggg ctcgcggaac caggcgccgt cgcgataatg
     7321 gaacagcccg aggccgccgg cgaagacctc gccgcgtccc gcgcccggga acacgtgcat
     7381 cgcggagatc ccgaaggccg cgagcgggaa cggctcgtcg cacagcgcgc tggtctgggc
     7441 gcgcccgtcg gatcccatcg cgtagcggtc gggacagaag ctggtgatcc tgccgtcgcg
     7501 gccgacgaac agcgcgaacg gccgggtcgg gtcgcccgcg gcgctggcct gcgcggtggc
     7561 ggcatccacg ggcacgatcg cgccgccgat ccacgcgccc gccgcggacg ccgcgacggt
     7621 gttcgcgggg gtggtcgcca cgatcgtggt tccgaccacg cgcgtcgctt cgccgtcgaa
     7681 catttcgcgg ccggtcatca cacgcgtcca gccggtgtcg ccgcgacggg cgatcagcgc
     7741 cgcgcggccg acgcgccccg cgatccaagc ttgctgcgcc gacacgaccg aaaccgagcg
     7801 aagatcgatg tcgtcggcgg cgatgccgag cgggtccggc agccagcgat cgccgtcgag
     7861 ccgcagcacg gtcgggccgc cgccgatcgc gaagccgatc gtgcgccccc cggccgaggt
     7921 gatcgacacc gacgacaggc tcgcgtcggt gacgcgcgcc gcctcgatcc acgtgccgcc
     7981 cgacaggcgg agcatggtgc cgccgccgcc gacggcccag ccggcgccgc cgggggcgag
     8041 cgagagcgcg gtgatcgtcg ggttgatcgg cgtgccgccg gccgagcgcg gcgggcctag
     8101 cagtcgccag cccgtcgagc gcgtgtagcg caggaacacc gtctggccgc cgccgtggtc
     8161 gccggcccag ccgggaacct tcgcggtgga gcgtccgaca gcccaggcct cgtgagaggc
     8221 gctgccgggc acggcaccga cgaggccggc ccgcacgccc agcgtcaccg gccggacggc
     8281 cgcttgctcc gagccgttga tgcccgatcg gtcgtagcgg ccggcccggg ttcgggccgg
     8341 agcctcggcc ggcagcagcg tgacgatcgc agacagcgcg atcaggccgc cgagcagggg
     8401 ccggctaacg gagtgtcgcc tgattcgggt catgacttcg cgcgagactc aaacaattcg
     8461 gtgcccgtct tcatgttcct tgcagaaagg accgcccgga gcggttcttc tttcaaggta
     8521 tcgcacgatt gacgggactt gacccttttg tgcgtcgcag gaaggccggg aacggtggcg
     8581 aaaatcatcg tcatgcgacg caccgccgtg acgctcatcg cttgcttcgc tctggccgcc
     8641 tgcgggggcg gagcctccga tccggccggc cacgggcgcg gaccagacgg gtcccccgat
     8701 tccctggcct cacccggggc ctcggcgggg acgggcgctc cgtccggccc gggggttccc
     8761 gccgcgcccg gaaccgtcac gctgccgccg gaggccacgg cccggccctc ggactttccg
     8821 gttccggact tcgtgaaggc ggaggtaccc gaggacgagg cgtgggga
//
LOCUS       DATLFO010000170         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_122397, whole genome shotgun sequence.
ACCESSION   DATLFO010000170 DATLFO010000000
VERSION     DATLFO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122397"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..375
                     /locus_tag="VM841_06840"
     CDS             22..375
                     /locus_tag="VM841_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89934.1"
                     /translation="MRLIGRRRRRRGSRRSRCRTRRRRHGEAVAIGMVFAAHLARIRG
                     MSDLIAEHVDAIESAGLPVGGADAPFEAVVEAMRRDKKYDRGIRFILLNAIGDAAVVS
                     GIETDEIRAAYDAVR"
     gene            372..803
                     /locus_tag="VM841_06845"
     CDS             372..803
                     /locus_tag="VM841_06845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HVL89935.1"
                     /translation="MSRILVLNGPNLDRLGMRQPEVYGTTTLAQIEARLRAQAAEHGH
                     EITFVQSAREDDLLRAIGAGADGIIVNPAAFTHFSYALADALAASALPVVEVHLSNIH
                     AREPYRRLSVVSPVARAVICGAGPAGYGYALDLLARILSES"
     gene            800..1897
                     /locus_tag="VM841_06850"
     CDS             800..1897
                     /locus_tag="VM841_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVL89936.1"
                     /translation="MSDHHPMHTSPINTKARRDAVRARLEELGADALLVTKPVNVRYL
                     TGFSGSNGQLVVGEEDIFFTDSRYEEQSRHQVPDCRREIFDSRSGWDRIVPELGTAAR
                     IAIEAHHLTVASAGRLKAALGDGRTLVEAENVVESRRLIKDAGEIERIRRACAFGDAG
                     FQEILGRLREGMTEVELAAELEDAMRRAGSEGLSFDTIVAFGESAAEPHHEPIERRLK
                     RGDVVKLDFGATFEGYHSDMTRTIAFGDPGEEMRTVYEAVRAGQQAGLEAVKDGASSG
                     DVDEASRAPIRAAGYDYGHATGHGCGLEVHEAPTVRSKGADILAPGMTVTVEPGIYLP
                     GLGGVRIEDLLAVTADGYDVLTTSPKELIVV"
     gene            1935..2498
                     /gene="efp"
                     /locus_tag="VM841_06855"
     CDS             1935..2498
                     /gene="efp"
                     /locus_tag="VM841_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HVL89937.1"
                     /translation="MATSNDLKNGMVLEIDGGLWSVMWFQHHKPGKGQAVVRTKMRNV
                     KTGAVVERTLNAGVKVDVAVIDKREMTYLYHDGTDWIFMDSQSYDQNPIPEEVLGDAT
                     KWLTENQTVLVGVHDGKPITVELPAAVELTVTQTDPGLKGDRVSGATKPATLETGAVV
                     QVPLFVEEGTRIRVDTRTGDYVTRVSD"
     gene            2512..>2800
                     /locus_tag="VM841_06860"
     CDS             2512..>2800
                     /locus_tag="VM841_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVL89938.1"
                     /translation="MPATFTHPRTASRRRALDILFSADVTGTDVLAVLADARRVDNAA
                     RSIVEGVAAHREEIDGLIRSYADRWSLERMPVVDRNLLRIGLYELLHEPGVP"
BASE COUNT          464 a          991 c          940 g          405 t
ORIGIN      
        1 ttgggtccgt tcagaaccag gatgcggctc atcggacggc gtcgtaggcg gcgcggatct
       61 cgtcggtctc gatgccggac acgacggcgg cgtcacggcg aggcggttgc gatcggcatg
      121 gtcttcgcgg cgcacctggc acgcatccgc ggcatgagcg acctgatcgc cgagcacgtc
      181 gacgcgatcg agtcggccgg cctgccggtg ggcggagcgg acgcaccgtt cgaggcggtc
      241 gtcgaagcca tgcggcgcga caagaaatac gaccgcggca tccgcttcat ccttttgaac
      301 gccatcgggg acgccgccgt cgtgtccggc atcgagaccg acgagatccg cgccgcctac
      361 gacgccgtcc gatgagccgc atcctggttc tgaacggacc caacctcgac cggctcggca
      421 tgcgtcagcc cgaggtctac ggcaccacga cgctggcgca gatcgaggcg cgcctgcggg
      481 cccaggccgc cgagcacggc cacgagatca ccttcgttca gtccgcgcgc gaagacgacc
      541 tcctgcgcgc gatcggcgca ggcgcggacg ggatcatcgt caatccggcc gctttcacac
      601 acttctccta cgcgctcgcc gacgcgctcg ccgcctcggc cctgccggtc gtcgaggttc
      661 acctgtccaa catccacgcc cgcgagccct accgccgcct gtcggtggtc tcgcccgtcg
      721 cccgggccgt gatctgcggc gcaggcccgg ccggatacgg ctacgcgctg gacctgctgg
      781 cgcgtatcct gtcggaatca tgagcgacca ccacccgatg cacacgtccc cgataaacac
      841 caaggcccgc cgcgacgctg ttcgcgcccg cctcgaagag ctgggcgcgg atgccttgct
      901 cgtcaccaag cccgtcaacg tccgttatct caccggcttc tccggctcca acggccagct
      961 ggtcgtgggc gaggaggaca tcttcttcac cgactcgcga tacgaggagc agtcccggca
     1021 tcaggttccc gactgccgcc gcgagatctt cgactcacgc agcggctggg accggatcgt
     1081 gcccgagctc ggcacggcgg cgcggatcgc catcgaggcg caccatctga ccgtcgcctc
     1141 cgccggacgg ctcaaggcgg cgctcggcga cggtcgtacg ctcgtcgaag ccgagaacgt
     1201 cgtcgagagc cggcgcctga tcaaggacgc cggcgagatc gagcgcatcc gccgcgcgtg
     1261 cgctttcggt gacgccgggt tccaagagat cctgggccgc ctccgcgaag gcatgaccga
     1321 ggtggagctc gccgccgagc tcgaggacgc gatgcgtcgc gccggcagcg aaggcctgtc
     1381 cttcgacacc atcgtcgcgt tcggcgagtc cgcggccgag ccgcaccacg agccgatcga
     1441 acgccgcctc aagcgcggcg acgtggtcaa gctcgacttc ggcgcgacct tcgagggcta
     1501 tcacagcgac atgacccgca cgatcgcctt cggcgatccg ggcgaggaga tgcgcacggt
     1561 ctacgaggcg gtccgcgcgg gccagcaagc ggggctcgag gcggtcaagg acggcgcgtc
     1621 gtccggcgac gtggacgagg cgtcgcgcgc cccgatcagg gccgcgggct acgactacgg
     1681 acacgcgacc ggccacggct gcggcttgga ggttcatgaa gccccgacgg ttcgctccaa
     1741 gggcgctgat atactcgcgc ccggcatgac ggtgaccgtc gagcccggga tctacctgcc
     1801 gggactgggc ggcgtccgga tcgaggacct cctcgccgtc acggcggacg gatacgacgt
     1861 cctgaccacg tccccgaaag agctgatcgt cgtctgacgc cgcgcccggg tgcgcgcggc
     1921 gcgaaaggaa cgcaatggca acctcgaacg atttgaagaa cggaatggtg ctcgagatcg
     1981 acggcggtct gtggagcgtg atgtggttcc agcaccacaa gcccggcaaa ggtcaggcgg
     2041 tcgtgcgcac caagatgcgc aacgtcaaga ccggtgccgt cgtcgagcgt acgctcaacg
     2101 cgggcgtgaa ggtggacgtc gcggtcatcg acaagcggga gatgacctat ctctaccacg
     2161 acggcaccga ctggatcttc atggactcgc agtcctacga ccagaacccg atcccggaag
     2221 aggtcctcgg cgacgcgacg aagtggctga ccgagaacca gaccgttctg gtgggagtgc
     2281 acgacggaaa gccgatcacc gtcgagcttc ccgccgctgt cgagctgacg gtcacgcaga
     2341 ccgatccggg cctgaagggc gaccgtgtct ccggcgcgac caagcccgcg acgctcgaga
     2401 cgggcgcggt cgtgcaagtg ccgctgttcg tcgaagaggg cacgaggatt cgcgtcgaca
     2461 cccgcaccgg cgactacgtc actcgcgtca gcgactgacg cgggatcggc cgtgccggcg
     2521 acgttcacgc accctcgcac cgcgtcgcgg cgccgcgccc tcgacatcct tttctcggcc
     2581 gacgtgaccg gcaccgacgt gctcgccgtc ctggccgatg cgcggcgcgt ggacaacgcc
     2641 gcacgttcca tcgtcgaggg cgtggccgcc caccgcgagg agatcgacgg cctgatccgc
     2701 tcctacgcgg accggtggtc gctcgagcgc atgcccgtcg tggaccgcaa cctgctccga
     2761 atcggcctct acgagctgct ccatgaaccc ggcgtgcccg
//
LOCUS       DATLFO010000171        10829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_141698, whole genome shotgun sequence.
ACCESSION   DATLFO010000171 DATLFO010000000
VERSION     DATLFO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141698"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VM841_06865"
     CDS             complement(<1..510)
                     /locus_tag="VM841_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89939.1"
                     /translation="MLRFKVVLAAFGILAAGAVPASAGHGSPILISTVAGGSGNGESD
                     MASLSGDGKIVAFQSTATNIVPNDTNGLSDIFIRDVPAGVTKRVSVTSSGAQIDGGRS
                     QNPAVSGNGRYVVFDTYFEALMLPFIYRHDLQTGQTIEVSDQGRRPAVSDDGDVVAYL
                     ETTATSLDMH"
     gene            689..847
                     /locus_tag="VM841_06870"
     CDS             689..847
                     /locus_tag="VM841_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89940.1"
                     /translation="MMTRIDEHGTGLGLVEAEDGGGRRWLGHSGSYAGFESELWHDGS
                     RGLTINNA"
     gene            complement(883..1308)
                     /locus_tag="VM841_06875"
     CDS             complement(883..1308)
                     /locus_tag="VM841_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVL89941.1"
                     /translation="MAKRHPRGLIDTSVLIDLERIDAAGLPAELAVSAVTMAELAAGP
                     HATTDRVERASRQDRLQRAEATFDPLPIDAAVARAYGRIYASVVARGRKPIGRRGFDL
                     LIAATALAAGLPLYTRNPKDFSGLGNLLIDIVSVPASPA"
     gene            complement(1301..1555)
                     /locus_tag="VM841_06880"
     CDS             complement(1301..1555)
                     /locus_tag="VM841_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HVL89942.1"
                     /translation="MTRQITQRELRNESGEIMRSLDRGEDFVVTRNGVPVGELRPVRE
                     RTYVTAERAVAAFAGAARIDAERFRRDIDAAADQDATPRG"
     gene            1741..2181
                     /locus_tag="VM841_06885"
     CDS             1741..2181
                     /locus_tag="VM841_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89943.1"
                     /translation="MRGVRLLVGALLVGGIIAGPGVGFAADCGAGGTTPNSSGSCKTA
                     TNQTVCGSGIDTGLIIVSAGPGGAEICNDGGTAFPVDGRAGAQLECVCVYIDGDDDNN
                     HDLLLHGWLRIDQNGAWCRDGRDPNGNGYGAPGRPAHECFVGPI"
     gene            complement(2390..4159)
                     /locus_tag="VM841_06890"
     CDS             complement(2390..4159)
                     /locus_tag="VM841_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL89944.1"
                     /translation="MNDNARSLASGRQAALLLVASGALTILNNYLPGAELLNRGVLNA
                     IGSAAVLLGIAAWVAPWERWHPRASLALAPAALTLIATANYLGGVSAYSYAVYFVVLF
                     VWIGTAHAPLTSLAIAPLAAIAYVTPFLVDDRLDGRAVSVTVAIPVCVLVGETVARAM
                     RRLRDAQQESERRAILLQTVADSARSMSVLDPRGVLEGVVSSALALGFDAAEICRFDH
                     DGSYRVMHSRGLPDEYIRGRHPAGSGLAAMVRDRGDIVVIDDYASFDAGVPAIRSGGF
                     RVVVGAPIWSGGVVAAALIAGTHARQGPPDYAEAFALLSAHAGRALENAERFSAERRA
                     VQHLAHLDRLKDEFISTASHELRTPVTVVEGMGLTLAWKWDALDDRTRRELLERMNLN
                     AHRLSRIVTSLLDFSRMESGAEADIQPVQLRPLIDECTTRLGAMLSGHGVQVRVRRDV
                     MVRADPMLLDSVMENLLANAAKHTPAGTTVRIAAGTVNGAVVVTVADNGPGIAAEDLP
                     RLGERFFRPASQSGYLAPGTGLGLAFVQGALKLHASKLQIECPPTGGAVFSFALAPAS
                     ATPENERSAEGPSISRGTVPAPG"
     gene            complement(4483..5289)
                     /locus_tag="VM841_06895"
     CDS             complement(4483..5289)
                     /locus_tag="VM841_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HVL89945.1"
                     /translation="MKRLLPLFSAYRRRLGPIESDLEARRSDLASSFAEIARGLYSAH
                     TVKETLDRIVDASVRTIDGCSGAGISFIQNGDIVTPVWTEPTVVEVDEMQYTTGQGPC
                     LDAITHGETFYAEDLRTDTRWPDFGPRAAAAGLRSLMSFCLAGEATLGALNLYARRPR
                     AFGATDRAMGLILATHAGVALAAAVELEDATKALEVETKRLQNLQGALFSRQVIGRAE
                     GILMNRERITGDQAFDLLRRASQQLNIKLREIAQHVVDTGEIPDRPGDAP"
     gene            5503..5748
                     /locus_tag="VM841_06900"
     CDS             5503..5748
                     /locus_tag="VM841_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89946.1"
                     /translation="MSPPTKNVSERTRDDSGLSAPELWRRYVELGGMRTSDEVDSILR
                     LEILPTDHDHDVLAHALNERFSELGRNHPVRYWGAAG"
     gene            5817..7655
                     /locus_tag="VM841_06905"
     CDS             5817..7655
                     /locus_tag="VM841_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVL89947.1"
                     /translation="MSEFARTMVTDFPIQAILDHLVRRIVDVLPITAAGVTLISAGTD
                     PRYVAASDQSALRFEELQTELGEGPCLMAYRTGTSVAISDLRDETRFPVFTPRAVAAG
                     LVAAFTFPLRSDRGVVGALDLYRDTPGPLDAASMDAAQTLADVAAAYLLNAQARADLQ
                     DSSDRSRERSLHDALTGLPNRVLLLERLDHAVRRSSRSGKIAAVLFIDLDRFKQVNDL
                     FGHGVGDELLVAVAQRVGAVLRPGDTLARLSGDEFVIVCEDLDAPGEAETIAGRIHSA
                     LGRPFELSGANVEITASVGIGFSGPGGQLSEELLGNADAAMYEAKRKGGGRQQIIDLR
                     DRRPVATSAGLARDLQHALVRAELRTLYQPIVDAGTGRIVAAEALVRWIHPVRGSVPP
                     SLFIPHAERSGLINPIGRWVLRQACIDRARLQRTRPEIDLSMSVNVSVQQLMSGEFAA
                     DVGDVLAETHTEPRLVTLELTESVFVQDSERAVVVLGRLKDLGVLLALDDFGTGFSSL
                     NHLKRFPVDMVKIDQTFVADLQHNQAGEAIALAIIELAHSLGMRVVAEGVETLAQLAR
                     LSSFGCDQCQGYFCAPPLSVDDFESMLLDPGAAQAMTRLLDAEARS"
     gene            7652..8389
                     /locus_tag="VM841_06910"
     CDS             7652..8389
                     /locus_tag="VM841_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HVL89948.1"
                     /translation="MSGSAMQPRATGHARLGLLGSFSLHIGSEPVRLPFNAQRLVCFL
                     ALHEGDLLREHVAGSLWSDATERRAAGSLRSALWRLGHPTDPVVEITDAHLRLSQGVV
                     VDVASAEALARRILDDGIELNEADMDEVSLGKDVLPDWTEDWVLVRREYHTQLRLRAL
                     EALCRRLSAIGRHGQATQAGLMAVSAEPLRESAQRVLIAASLAEGNIAAARKQYDSFR
                     DLLRKELDLEPSPDMRALIEAASITAR"
     gene            8414..8656
                     /locus_tag="VM841_06915"
     CDS             8414..8656
                     /locus_tag="VM841_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89949.1"
                     /translation="MKPTLERTGVLIVRLWIEVNHDQGLRARITQTLDTGAGEQSIAV
                     ATSAEGICAVVKEWVQDFATLGPSGGNGRGGPAEEA"
     gene            8783..9226
                     /locus_tag="VM841_06920"
     CDS             8783..9226
                     /locus_tag="VM841_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL89950.1"
                     /translation="MARLDIMTEMGMMAGGMKHPLLSVSGEQRGHAAHLHVSGEIDLA
                     TAPVLEGWLDTAERNGNRAIVVDLEHVTFIDASGLRAFLHAAQRASLAGRTLELTNAP
                     AVVRRVLQLTGCTHLLAADPLAQSPYERKPAPLAAQTIVARPSIQ"
     gene            complement(9371..>10829)
                     /locus_tag="VM841_06925"
     CDS             complement(9371..>10829)
                     /locus_tag="VM841_06925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVL89951.1"
                     /translation="KGRDPGFLRHGATAFKGRVITKQPATLPQNTAVPYRGTPLSFVS
                     TYIGRKAAEPTIGIDKKGGIVFPAAAFDALPDGSPRQLAHTLYRASFDNGKTWKTVQP
                     PIVPGTETDGEPTSLDPYVYVDTDSGRWFGVDLKGVGTSIHFSDDQGKTWTKSFASNP
                     GFNDHQTLASGPLPKDSALVTTDPIFKNVVYYCTNQVYSALCMRSLDGGLTFTQAGQP
                     YPPVDPTATPPDRIPFCGSLHGHVIVDPEGRVVVPRGHCGLGWVSVSEDAGVTWTMTK
                     VSDTIGSDDTDAAVASDAAGNLYFTWLDSGWQLPYLSVSRDHGKTWSAPRMIAPPGVR
                     DAWVPTIVAGDAGRVAIAFVGSPSNDRTDMSRPWNQYIVMTSDGLSKDPLFLSNTANP
                     GGPGDPIHRGFCNQRCGNLYDFIDIQVAPDRNGTIWVATADTCTTLRKCNTVRAPGAT
                     GIILGPQDGVSADMQGVAIRQLGGPSMRAPKPRRR"
BASE COUNT         1822 a         3504 c         3680 g         1823 t
ORIGIN      
        1 gtgcatatcc aagctcgtcg cggtcgtctc gaggtaggcg acgacatctc cgtcgtcgga
       61 caccgccggc cgccggcctt gatcgctcac ctcgatcgtc tgtcccgttt gcaggtcgtg
      121 ccggtagatg aacggcaaca tgagcgcctc gaaatacgtg tcgaagacca cgtaccggcc
      181 gttccccgac acggccgggt tctgcgaacg accgccgtct atctgtgcgc ccgatgacgt
      241 gaccgagacg cgcttggtca ctcccgccgg aacgtctcgg atgaagatgt cgctgagacc
      301 gttcgtatcg ttcggcacga tgttggtcgc cgtggactgg aaggcgacga tcttgccgtc
      361 gccggagagc gaggccatat cgctttcccc gtttcccgac ccgccggcaa cggtcgagat
      421 caggatcgga gacccgtggc cggcggacgc cggaactgct cccgcggcca ggattccgaa
      481 tgcagccaaa acgaccttga atcgaagcat ggcttcctcc cttttcggtt ggatgggagt
      541 ttggcaacgc cgccggcttc gccgcgtcgg ccgatcggcc gaacttttgg gccgcggggc
      601 ccacgagtga gggcgggacg ccaccgcgcc cgggggcctc gtccgctccg ccggcggttg
      661 ccgaagacgc gcggaggcga ccctggccat gatgacccgc atcgacgagc acggcactgg
      721 gctgggtctg gttgaagccg aggacggagg cggccgcagg tggctgggcc acagcggatc
      781 gtacgccggg ttcgaaagcg agctgtggca cgacggctcg cgtggcctga ccatcaacaa
      841 tgcatgagcg ggagaccgga cgtcccggcg ggccgtcgca ggctatgccg gactggccgg
      901 aaccgagacg atgtcgatga gcaggttgcc gagccctgag aagtctttgg ggttgcgcgt
      961 gtaaagaggg agaccggcgg cgagtgccgt tgcggcgatg agaaggtcga acccgcggcg
     1021 cccgatgggc ttgcggccgc gagcgacgac ggaggcgtag atcctgccgt atgcgcgcgc
     1081 gacggccgcg tcgatcggca gcggatcgaa tgtggcctcg gcgcgctgga gccggtcttg
     1141 ccggctcgcg cgttcgactc gatcggtggt ggcatggggg ccggcggcga gctcggccat
     1201 cgtgacagcc gacacagcga gctcggcggg caggccggct gcgtcgattc tctccaaatc
     1261 gatcaggacg gacgtgtcga tgagtccccg cgggtgtcgc ttagccacgc ggcgtggcat
     1321 cctggtcggc ggccgcgtcg atgtcgcgcc ggaaccgctc ggcgtcgatg cgcgccgcgc
     1381 ctgcgaaagc cgcgacggct cgctcggccg tgacgtaggt tcgctcccgt accgggcgta
     1441 gctcaccgac gggaacgccg ttgcgcgtaa ccacgaaatc ctcccctcga tccaaggagc
     1501 gcatgatctc accgctttcg ttgcggagct ctcgctgcgt gatctgtcgc gtcatactcc
     1561 aatggtagca cgcgtgctac ggggcgggtc cgctcgatcc gcgcccaccc aggatgtcga
     1621 aagcaggcac cgtgtcccga gcgggcagag tcggcgaaac ggcgcgaaga ccgatcgcgc
     1681 ggaggagatc ggcggtgcgg cggcgaagat cccgggactg agcttcggag gagggtattg
     1741 atgagaggcg ttcggttgct ggtcggggca ctgcttgtgg ggggcatcat cgcgggtccg
     1801 ggagtgggat tcgccgcgga ttgcggcgcg gggggcacga ctcccaacag cagcggctcg
     1861 tgcaagacgg cgacgaatca gaccgtgtgt ggatcgggca tcgacacggg gctgatcatc
     1921 gtgtcggccg ggccgggcgg cgccgagatc tgcaacgacg gcgggacggc gttcccggtc
     1981 gacgggcgcg cgggtgcgca gctggaatgc gtctgcgtct acatcgacgg cgacgacgac
     2041 aacaaccacg acctgttgtt gcacggatgg ctccgcatcg accagaacgg cgcctggtgc
     2101 cgcgacggcc gcgatcccaa cggaaacggc tacggtgctc cgggccggcc ggcccacgaa
     2161 tgcttcgtcg gtcccatcta gcacgaccgc cgaatcatcg aagacgcgga cccgattgat
     2221 ttacgtctga cgaccgagga tccagccttc gacttgtgta acggaggggt cgtcccacgg
     2281 tgtctttccg ctctttcctt tcgatatttg gatagcgccg cgatgggtgc gatcgtcgcg
     2341 atcaatcggg cgctgctcga acgcccccca ggggacacct ttgaagatct catccaggtg
     2401 ccgggaccgt ccctcgggaa atagatggtc cttcggcact tcgctcgttt tccggcgtcg
     2461 cagaggccgg cgccagcgcg aacgaaaaga ccgctccgcc ggtgggagga cattcgatct
     2521 gcagtttgct ggcgtgaagt ttgagcgccc cctggacgaa ggccaggcca agacccgtcc
     2581 ccggggccaa atagccggat tgcgaggctg ggcgaaagaa tcgttcgccg aggcgtggca
     2641 gatcctcggc cgcgatgccg gggccgttgt cggcgacggt cacgaccacc gcgccgttga
     2701 cggtgccggc cgcgatgcgc acggtggttc cggcaggggt gtgcttggcg gcgttggcca
     2761 gaaggttctc catcacgcta tcgagcagca tcggatccgc ccgcaccatg acgtcgcgcc
     2821 tcacgcgcac ctgaacgccg tggccggaca gcatggcacc cagcctcgtc gtgcattcgt
     2881 cgatcagcgg ccgcagttgc acgggctgga tgtcggcctc ggcgccggac tccatccgcg
     2941 aaaagtccag cagcgacgtc acgatgcgcg ataagcgatg cgcgttgagg ttcatccgct
     3001 cgagcagctc gcggcgggtc cggtcgtcga gcgcgtccca cttccaagcc agggtcaggc
     3061 ccattccttc gaccaccgtg acgggtgtgc gcagttcatg tgatgccgtc gatatgaatt
     3121 cgtccttgag ccggtccaga tgagcgaggt gctgcaccgc gcggcgttct gccgagaacc
     3181 gctcagcgtt ttcgagcgca cgaccggcat gcgcggaaag caatgcgaag gcctcggcat
     3241 aatctggcgg gccttgacgg gcatgcgtgc cggcgatcag cgcagcggca acgacaccgc
     3301 cactccagat gggggcaccg acgacgacgc ggaacccacc actgcggatg gccggtacgc
     3361 cggcgtcgaa gctggcgtag tcgtcgatga cgacgatgtc gccacggtcg cgcaccattg
     3421 ccgcgaggcc cgatccggcc gggtgtcggc cgcggatgta ttcgtcgggc aggcctcgcg
     3481 agtgcatcac acggtagcta ccgtcgtggt cgaaccggca gatctcggcg gcgtcgaaac
     3541 cgagcgcgag cgccgaggag acgacacctt cgagcacgcc ccgcgggtcg agcacgctca
     3601 tcgaccgggc tgaatcggcg acggtctgca gcaagatcgc gcggcgctcg gattcttgct
     3661 gcgcatcacg gaggcggcgc atcgctcgcg ccaccgtttc gccgaccagc acgcacaccg
     3721 ggattgccac ggttaccgac accgctcggc cgtcgagccg atcatcgaca aggaatggcg
     3781 tcacgtaggc gatcgcagcg agtggtgcga tcgccagcga ggtgagcggc gcatgcgcgg
     3841 tgccgatcca gacgaagagc acgacgaaat acacagcgta cgaatacgcc gataccccgc
     3901 cgaggtagtt ggccgtagcg atcagcgtca acgccgccgg cgcgagggcg agcgaagcac
     3961 gcgggtgcca ccgctcccat ggcgctaccc acgcggcgat gccgagcagg accgccgcgg
     4021 agccgattgc gttgagcacg ccgcggttga gcagctcggc gcccggaaga tagttgttca
     4081 gaatggtcag tgccccggac gcaacgagaa gcaaggcggc ctgacggcca ctcgcaagtg
     4141 agcgtgcgtt gtcgttcatc ggataagtcc ggcagtacag aacacggcct gaggccgggt
     4201 cgggcaatag ctcgaactga cggtctacgc ccgtccttgg tggctggtta ccgccgagtc
     4261 cgttccgatg aggcctgctc ggagccgagg ccgcagtggc cgcgggaaac acgatgccgc
     4321 ccttcttgtc gatgccgatg gccggctcca ggcgactcgt caagtttctt gacgagtctg
     4381 ttcggaacac atccgggaca gactgggcgg gcgcgccccg gcggcatcgg gattgcggtg
     4441 ccggtcgtcc agccaccggc tcgcgttctt ccgcgcggcc atctacggtg cgtcgccggg
     4501 acgatcaggg atctcgccgg tgtccacgac gtgctgcgcg atctcgcgaa gcttgatgtt
     4561 gagttgctgg gatgcgcgac gcagcagatc aaatgcttga tcaccggtga tgcgctctcg
     4621 gttcatcagg atcccttcgg ccctcccgat cacctgtcga gaaaagagag ccccttgcag
     4681 attctgcagt ctcttggttt ccacctcgag tgccttcgtc gcatcctcga gctccaccgc
     4741 ggcggcaagc gcgacgccgg cgtgagtcgc caagatcaaa cccatcgctc gatcggtcgc
     4801 tccgaaagct cgcggccggc gggcgtacag gttgagtgcc cctaaggtcg cctcgccggc
     4861 caggcaaaac gacatcaggc tgcgcaaccc ggctgcggcg gccctgggac cgaaatcggg
     4921 ccagcgtgta tcggttcgaa gatcctcggc atagaaggtc tcaccgtggg tgatcgcgtc
     4981 aaggcaaggc ccttgaccgg tggtgtattg catctcatcg acctcgacga ccgtgggctc
     5041 ggtccacacc ggcgtgacga tgtccccgtt ctggatgaac gagatcccgg cgccggagca
     5101 cccatcgatc gtgcgcaccg aagcgtccac gatgcggtct aaggtttctt tgaccgtgtg
     5161 cgccgagtac aggccgcgag cgatctcggc aaagcttgac gcgagatcgc tccggcgtgc
     5221 ttccagatcg ctttcgatcg gccccaacct cctccggtaa gccgagaaga gcggcagcag
     5281 ccgcttcacg accgaatata gattgtatcc cccctggcat ccatgaatcc tccgcgcccg
     5341 aatccccatg gccggccgct gcgcagggtg tatgatggag acgccttcac cagcgagggt
     5401 tccccgaccc gggcagcgat gcccgacgag aatcccgagg tgaaggttgt tgttttcccc
     5461 ggcgtttctc cgcagaacgc ctctacttga gtcgccgacg cgatgagccc gccgaccaag
     5521 aacgtgtcgg agcgcacacg cgacgactct ggactgtcgg cgcccgagct gtggcgacgc
     5581 tacgtcgagc tcggcgggat gaggacctcc gacgaagtgg actcgatctt gcgtctggag
     5641 atcctgccga ccgaccacga tcacgacgtg cttgcccatg ccctgaacga acggttcagc
     5701 gagctcggcc gcaatcatcc ggtccgatac tggggggccg caggatgagc cggcgatcgg
     5761 gcgtgccttc cgtcgagcgg gatcggatgt cgagcgagca acaactgtcg gatgtgttga
     5821 gcgaattcgc gcgcacgatg gtgacggact tccccatcca agcgatcctc gaccatctcg
     5881 tgcggcgtat cgtcgacgtc ctgcccatca cggcggccgg cgtcacgctg atctccgcgg
     5941 ggacggaccc gcgctacgtg gccgcctcgg accagtcggc gcttcgcttc gaggagttgc
     6001 agaccgagct cggggagggg ccgtgcttga tggcgtaccg aaccggcacg tcggttgcga
     6061 tctcagacct tcgcgacgaa acacgcttcc ccgtgttcac acctcgtgcc gtggccgccg
     6121 ggctcgtagc ggccttcact tttccgctgc gaagcgaccg aggcgttgtc ggtgcgctgg
     6181 acctgtatcg ggacaccccc gggccgttgg atgcggcgtc catggacgcg gcgcaaacat
     6241 tggccgacgt cgccgccgcc tatctgctca acgcccaagc gcgagccgac ctgcaagact
     6301 cttcggatcg gtcacgcgaa cggtcgctgc acgatgcgct caccggcctt cccaaccgcg
     6361 tgctgttgtt ggagcgcttg gaccacgccg ttcggcgcag ctcccgctcg gggaagatcg
     6421 ccgcggttct ctttatcgat ctcgaccggt tcaagcaggt caacgatctg ttcgggcatg
     6481 gcgtcggaga cgaactgctc gtcgccgtcg cacagcgggt cggcgccgtg ctgcgcccgg
     6541 gcgacaccct tgcgaggctg tccggcgacg agttcgtcat cgtgtgcgag gacctcgacg
     6601 cgcccggcga agccgagacg atcgccggcc ggatccacag cgcgctggga cgaccattcg
     6661 agctgtcagg agcgaacgtc gagatcaccg ccagcgtcgg catcggcttc tcgggcccgg
     6721 gcggacaact ctcggaagag cttctgggaa acgccgacgc ggcgatgtac gaagccaagc
     6781 gcaagggggg cggccgccag caaatcatcg acctgcggga ccggcgtccg gtcgcgacct
     6841 cggccggcct cgcacgcgac ttgcaacacg ccctcgtccg cgccgagctg cggacgctgt
     6901 accaacccat cgtggatgcg ggcaccggcc ggatcgtggc cgccgaagct ctcgtgcgtt
     6961 ggatccatcc cgtccgcgga tcggtgccgc catcgctgtt cattccccac gccgaacggt
     7021 ccggcctcat caacccgatc gggcgatggg tgctccggca ggcctgcatc gaccgagcgc
     7081 gccttcagcg aactcgcccg gaaatcgatc tgtcgatgtc ggtgaacgtt tcggttcagc
     7141 agctcatgtc gggcgagttc gccgcagacg tcggcgatgt tttagccgag acgcacaccg
     7201 aaccccggct cgtgaccttg gagctgaccg aaagcgtgtt cgttcaagac agcgaacgcg
     7261 cggtcgtcgt cctgggccgg ctcaaggacc tcggggtcct gctcgcgctg gacgatttcg
     7321 ggaccggctt ctcctcgctc aaccacctca aacggttccc ggttgacatg gtcaagatcg
     7381 atcagacctt cgtcgccgac ctgcagcaca accaggccgg cgaagcaatc gccctcgcca
     7441 tcatcgaact ggcccatagc ttgggcatga gggtggtggc ggaaggagtc gagaccctcg
     7501 cgcagctcgc acggctctcc tcgttcggct gcgaccaatg tcaaggctat ttttgcgctc
     7561 ctcctctgtc ggtggacgat ttcgaatcga tgcttctcga tcccggagcc gcacaggcca
     7621 tgacacggct tcttgacgcg gaggctcgat cgtgagcgga tccgcgatgc agcctcgggc
     7681 cacgggacat gcgcgcctcg ggctgctggg ctcgttcagc ctccacatcg gctccgagcc
     7741 ggtgcggctg ccgttcaacg cccagcggct ggtctgtttc ctcgcgctcc acgaagggga
     7801 cttgcttcgc gagcacgtag ccggatcgct ctggagcgac gcgacggaac gccgtgccgc
     7861 gggcagtctt cgctcggcgc tctggaggct cggccatccg accgatcccg tcgtggaaat
     7921 aaccgacgca cacctgcggc tttcccaggg ggtcgtggtg gacgtcgcgt cggctgaagc
     7981 gcttgcgcgc agaatcctgg acgacgggat cgagttgaac gaggccgaca tggacgaggt
     8041 gtcgctgggg aaagacgtcc tgcccgactg gacggaagat tgggtcctgg tcagacgcga
     8101 atatcacacc cagttgcgcc tgcgcgccct ggaagccctc tgccgcaggc tcagcgccat
     8161 cggaaggcac gggcaggcga cgcaggccgg cttgatggcg gtgtccgccg agccgctgcg
     8221 cgagagcgcg cagcgcgttc tgatcgcggc cagcctggcc gaggggaaca tcgccgccgc
     8281 ccgcaagcaa tacgactcgt tccgagacct ccttcgcaaa gagctcgatt tggagccgtc
     8341 accggacatg cgggcgctga tagaggctgc atccataaca gcgcggtgac ggccgtggtg
     8401 caccatccgt ctcatgaaac caacactcga gcggacgggg gtgctgatcg tgcgcctgtg
     8461 gatcgaagtg aaccacgacc aggggctgcg cgctcgcata acccaaactc tggacacggg
     8521 agccggcgaa cagtcgatcg ctgtagcgac gtccgccgag ggcatctgcg cggtcgttaa
     8581 agagtgggtg caggactttg ccacgctcgg cccgtccggc ggaaacggac gcggcggtcc
     8641 ggcggaggaa gcgtaagcca cggtcctgtg acgcgaccgt gacaggcgcc gatcacgatg
     8701 gttgagagaa tgaaaagagc tcgcatcaga gcagagggag aagtcaatga tgagtactca
     8761 cgctgagggt ttgggcgcag gcgtggctcg cctcgacatc atgacggaga tgggcatgat
     8821 ggccggggga atgaagcacc ctcttttgag tgtctccggt gaacagcgtg gccatgccgc
     8881 gcatctgcat gtgtccggcg agatcgacct ggcgaccgca ccggtcctgg aggggtggct
     8941 ggacacagcc gagcgaaacg gcaacagggc gatcgtcgtc gatctggagc acgtcacgtt
     9001 catcgatgcc agcggcttgc gcgccttcct gcatgcggcg cagcgggcaa gcctagccgg
     9061 acgaaccctc gagctgacga acgctccggc cgtcgtgcgg cgggttcttc agctcaccgg
     9121 gtgcacccac ctgcttgccg cggacccgct cgcgcagtcg ccctacgaac ggaagccggc
     9181 accgctcgcg gcccagacca tcgtcgcgcg accgagcatc caatagggcc gcccgcgcga
     9241 cgcccgtgac ctgctgcctg acatcgggac gggaggccgc cgttgcgaac gcactcgtca
     9301 agtttcttga cgagtgcgtt cggagccaca tccgggacgg gccggggtgg ctgttgtctc
     9361 ctccgctgac ctaccgccgc cgcggcttgg gagctcgcat cgacgggccg cccaactgac
     9421 ggatggccac gccttgcatg tcggccgaaa ccccgtcctg cgggccgagg atgatgccgg
     9481 tcgcacccgg cgctcgcacg gtgttgcact tcctcagcgt ggtgcaggtg tcggcggtgg
     9541 cgacccagat cgttccgtta cggtcgggcg cgacctggat gtcgatgaag tcgtagaggt
     9601 tgccgcaccg ctggttgcag aagcctcggt ggatcggatc gcccggcccg cccgggttgg
     9661 cggtgttcga cagaaacagc ggatccttcg acaggccatc gctggtcatc acgatgtact
     9721 ggttccaggg ccgcgacatg tcggtgcgat cgttggaggg cgagccgacg aacgcgatcg
     9781 cgacgcggcc ggcgtcgccg gcgacgatgg tcggcaccca cgcgtcccgc actcccggcg
     9841 gcgcgatcat gcgcggcgcg ctccaggttt tgccgtggtc gcgcgagacc gacaggtacg
     9901 gcagctgcca tccgctgtcg agccaggtga agtagaggtt gccggccgcg tccgaggcga
     9961 cggcggcatc ggtgtcgtcg gatccgatcg tgtccgagac cttggtcatg gtccaggtga
    10021 cgccggcatc ttcgctgacc gagacccagc cgaggccgca gtgcccgcgc gggacgacga
    10081 cgcgtccctc gggatcgacg atcacgtgac cgtgcagcga tccacaaaac ggaatgcgat
    10141 ccggcggcgt cgcggtcgga tcgaccggcg gatagggctg gccggcctgc gtgaaggtga
    10201 gcccgccgtc gaggctgcgc atgcacagcg ccgagtagac ctggttggtg cagtagtaca
    10261 cgacgttctt gaagatcgga tcggtcgtga ccaaggccga gtctttcggc agcggccccg
    10321 atgcgagcgt ctgatgatcg ttgaatcccg ggttggaagc gaacgacttc gtccacgtct
    10381 tgccttgatc gtcgctgaaa tggatcgagg tgccgacgcc cttcaaatcc accccgaacc
    10441 agcggccgct gtcggtgtcg acatagacgt acgggtcgag tgaggtcggc tcgccgtcgg
    10501 tctcggttcc cgggacgatc ggcggctgaa cggtcttcca cgtcttgccg ttgtcgaacg
    10561 aggcgcgata cagcgtgtgc gcgagctggc gcgggcttcc gtccggcagc gcatcgaagg
    10621 ctgcggcggg aaacacgatg ccgcccttct tgtcgatgcc gatcgtcggc tcggctgctt
    10681 tgcgtccgat gtaggtgctc acgaacgaca gcggcgtgcc gcggtacggc acggccgtat
    10741 tctgcggcag cgtcgcgggc tgcttcgtga tcacgcggcc cttgaacgcg gttgcgccgt
    10801 gacgcagaaa gcccggatcc cggcccttc
//
LOCUS       DATLFO010000172         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_62927, whole genome shotgun sequence.
ACCESSION   DATLFO010000172 DATLFO010000000
VERSION     DATLFO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62927"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1114)
                     /locus_tag="VM841_06930"
     CDS             complement(170..1114)
                     /locus_tag="VM841_06930"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HVL89952.1"
                     /translation="MNATEVVRERLNVGDGGLRFGDARRIGPLTLIPLFATGAAAEYV
                     PYSIAQAEGLVSVKELGAAGSVPTLLVRNTGAAPVLLVEGEILIGLKQNRVLNVSVLV
                     PAASEVPIPVACVEAGRWHFNSSAGLKDQFHLSPQVRGVINVSVAGSARRQGDFMADQ
                     GAVWDGVDERLAAHGVSSATAAYSDIGAYRREEIMEKAAAARPEPGQVGVLAMVAGEP
                     LCLDLFDREETLATLWEGLVGSYVSDALAAPEEEAPLDMESVHAWIAGLAQAEVTEHP
                     GAALGRTVVATAPTGTAAGLVVGQALVHLSAVSRETTL"
     gene            1315..1941
                     /locus_tag="VM841_06935"
     CDS             1315..1941
                     /locus_tag="VM841_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HVL89953.1"
                     /translation="MPKKRKDDGLDVLVQVFAQLPWWLPFGFAIVALVGIPPLGRTLV
                     KQPGYDQVWGTLGMLVAVAMAGSGIFAQFEKRRRRKLVAQATAIDGLRTLSWRQFEEL
                     CAEAFRRRGYAVRETARGADGGVDLVLRRGNERIFVQCKHYAARRIDVRPIRELYGVM
                     AAEGATGGIFICSGRYTSAAQEFARGKRLELIGEPELLELLGVSATPL"
BASE COUNT          365 a          685 c          633 g          332 t
ORIGIN      
        1 cagttcgaga aattccgcca tcaacggctc gacgcgtcgg caaccgcgac cgcagtccca
       61 cggcggaacg gctgacgacc accgcgacgc tgacgttcgc gtgcgggtcc cccgcagatc
      121 tctccgcgga cctacccggc tctgcagtcg tctcactccc actacctctt cacagcgtcg
      181 tctctcggct gacggccgac aggtgaacca aagcctggcc gacgaccaat cctgccgcgg
      241 tccccgtcgg tgcggtcgct acgacggtcc gcccgagggc cgcaccggga tgctcggtca
      301 cctcggcttg ggcgaggccg gcgatccatg cgtgtacgga ctccatgtcc agcggcgcct
      361 cttcttcggg agcggcgagg gcgtcggaaa cgtacgagcc gacgagacct tcccacagcg
      421 tcgcgagcgt ctcttcgcgg tcgaacagat cgaggcacag cggctcgccg gcgaccatcg
      481 ccagcacgcc gacctggccg ggctccggcc gagcggcggc cgccttctcc atgatctctt
      541 cgcggcgata cgcgccgatg tcggagtagg cggcggtcgc ggacgagacg ccgtgagcgg
      601 cgagccgctc atcgaccccg tcccacactg ccccttggtc cgccatgaaa tctccttgcc
      661 ggcgcgccga tcccgcgacc gacacgttga tgacgccgcg cacctgtggc gaaaggtgaa
      721 actggtcttt cagtcctgcg ctggagttga agtgccaacg accggcctcg acgcacgcca
      781 ccgggatcgg cacctccgat gcggccggca ccagcacgct gacgttgagc accctgttct
      841 gcttgagccc gatgaggatc tcgccttcga ccaaaaggac gggcgccgcg ccggtgttgc
      901 gcaccagcag cgtcggaacc gagccggcgg ccccgagttc cttcaccgac acgagcccct
      961 ccgcctgggc gatcgaatac ggcacgtact cggccgcggc accggttgcg aacagcggga
     1021 tcaacgtcaa cggtccgatc cgccgcgcat cgccgaagcg aagcccgccg tcgccgacgt
     1081 tgagtctttc acgaacgacc tcagttgcat tcatcaacag ctccgttcat cggggaagtg
     1141 cgatcaggat cagcctagga accacccccg acaattacag cggtgtaatt ccctgaggcc
     1201 tgtcactgtc actggccaat gcaatgatgc cgcccatgac caccgtcaat gtgggcacct
     1261 cggccctaaa gccgcgggcg cgacattccg atcagacctc ggggggtgtt gatcatgccg
     1321 aagaagagga aagacgacgg actggacgtg ctggtccagg tgttcgcgca gctaccgtgg
     1381 tggcttccgt tcggtttcgc gatcgtggct ctggtgggca ttccgccact gggcagaact
     1441 ctggtcaagc agccgggcta cgaccaggtg tgggggactc tcggaatgct cgtcgcagta
     1501 gcgatggccg gctcgggaat cttcgctcag ttcgaaaagc gtcgccgccg caagctcgtc
     1561 gcgcaggcaa cggcgatcga cgggctacgg acattgtcgt ggcgccagtt cgaggaactc
     1621 tgcgctgaag cgttccgccg gcgcggttat gccgtccgcg aaactgcccg cggcgccgac
     1681 ggaggcgtcg atctggtgct gcgacgcggc aacgaacgca tcttcgttca gtgcaaacac
     1741 tatgcggcac gccgcatcga cgtgaggccg atccgcgaac tctacggagt gatggccgca
     1801 gagggtgcga ccggtggcat cttcatctgc tccggcagat acacgagcgc ggctcaagag
     1861 ttcgcgcggg gcaaacgtct ggaactcatc ggcgaacccg aactacttga gctcctgggt
     1921 gtatccgcga caccactttg agagggagcg ggcgacatgg aattcactaa aggcgagcac
     1981 gccgacagtc ctgcccgacc ccggcaatta catca
//
LOCUS       DATLFO010000173         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_93595, whole genome shotgun sequence.
ACCESSION   DATLFO010000173 DATLFO010000000
VERSION     DATLFO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93595"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..976)
                     /locus_tag="VM841_06940"
     CDS             complement(17..976)
                     /locus_tag="VM841_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL89954.1"
                     /translation="MRRLLLLLVSVLPATSTAVARPSTCVQINVPVSLTAGAPADQTI
                     WTALCRPAGAQTVHVLQSGATYGAAHWDFPYRPAQYSYVRALNGSGIATLNVDRLGIG
                     RSSHPPSALVTVDANAFVLHQLIAGLRNGGLGTNRFRPFAKVVTVGNSLGSVIAVTEA
                     ARYGDVDGVVVTGANKNGNARELGLFAAQATPALADPRFAGRIDDPGYLTLPDETRCR
                     TFWSDRDQDCDGPVATTDNALKETITAAEGIRAGTWVMTGEQGPGVPQDPDTSSIRVP
                     VLLVAGSEDLVECGEGTDCSDAESLRSSEGRYYPNAPSVEAFG"
     gene            complement(979..>2239)
                     /locus_tag="VM841_06945"
     CDS             complement(979..>2239)
                     /locus_tag="VM841_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89955.1"
                     /translation="RGHATFDAYGDCVYYPDNVSVAIGAGVVVLDVSDPSNPVKTDYL
                     TARAMRSPGESLRVNRERGLLVANYYAFPPPYTADGGIEPPEIMRALAVYDVSEDCAH
                     PRLLADVILPTQAGHEGCFQPDGMVYYSSAGTITPIDLTDPANPREMTVPWLPADPLP
                     AVFHGCSISDDGTRGYFADAANNQMLIVDTSEVQALIPGAQMRVLGRFSTPSELQQSA
                     IAITFDGAPHVLLFTEAKYPSKVCVPGQPNFGYPRIIDVGDETHPVEVAKIQAEVVLS
                     RNCSQVAADWAPQTRGADRGDAINVGISAVFFGGYDSHYCNVDRPRDPTLLGCGQMGS
                     GMRVYDLRDPHAPIEIAYYNTGTRSLDDPTLDWAWSPPVFRTDLGQIWWSTFAEGFHA
                     AKFRDGVMPLERDTPAIGTTIRSARGA"
BASE COUNT          379 a          721 c          772 g          367 t
ORIGIN      
        1 cgtccgaagg gcgctattac ccgaacgcct cgacggacgg cgcgttcggg taatagcgcc
       61 cttcggacga gcgcagcgat tcggcgtcgg agcaatcggt gccttcgccg cactcgacca
      121 gatcctcaga cccggcgacc agcaagacgg gaacgcggat cgaactcgtg tcgggatcct
      181 gcggcacgcc cggtccttgc tcaccggtca tgacccacgt cccggcgcgg attccttccg
      241 cggccgtgat cgtctccttc agcgcgttgt cggtcgtcgc gaccgggccg tcgcagtcct
      301 gatcgcggtc gctccagaac gtacggcaac gcgtctcatc cggaagggtg aggtagcccg
      361 gatcgtcgat gcgtccggcg aagcgaggat ccgcaagtgc cggcgtcgct tgcgcggcga
      421 agaggccgag ttcgcgtgcg ttgccgttct tgttcgcacc ggtaacgaca acaccgtcga
      481 cgtcgccgta tcgggccgcc tccgtcaccg cgatcacgga tcccagcgaa ttcccgaccg
      541 tcaccacctt cgcgaacggc cggaagcggt tcgtgccgag accgccgttt cgaagtccgg
      601 cgatcagttg atgcaagacg aacgcgttcg cgtcgacggt caccagggcg ctcggcggat
      661 ggctgcttcg accgatcccc aaacgatcga cgttcagggt cgcgatgccg gatccgttca
      721 gggcgcgaac gtacgagtac tgggccggcc ggtacgggaa gtcccaatgc gcggccccgt
      781 acgtcgctcc ggattgaagc acatggaccg tctgcgctcc ggccggccgg cacagcgcgg
      841 tccagatcgt ttgatcggcg ggtgctcccg cggtgagcga gaccggcacg ttgatctgca
      901 cacacgtcga aggccgggcg acggccgtcg acgtcgccgg cagaaccgaa accaacagca
      961 acagcaggcg tctcatgatc atgcccccct tgcggaccgg atcgtggtgc cgatagcggg
     1021 cgtatcccgt tcgagcggca tgacgccgtc gcggaacttc gcggcgtgga acccttcggc
     1081 gaacgtcgac caccagatct gaccgaggtc ggtgcggaac acgggcggag accaagccca
     1141 atccagcgtc ggatcgtcca ggctccgcgt tccggtgttg tagtaggcga tctcgattgg
     1201 cgcgtgcggg tcgcggaggt cgtacacgcg catccccgag cccatctggc cgcaccccag
     1261 gagcgtgggg tctcgtggac gatcgacgtt gcagtagtgg gagtcgtacc caccgaagaa
     1321 gacggcagag atcccgacat tgattgcgtc gcctcgatcc gcaccgcgcg tctgcggggc
     1381 ccaatcggcc gccacctgcg agcagttccg ggacagaacg acctcggctt ggatcttcgc
     1441 gacctcgacc ggatgggtct cgtcgccgac gtcgatgatt cgcgggtaac cgaagttcgg
     1501 ctggcccgga acacagactt tggacggata cttcgcctcg gtaaacagca acacgtgggg
     1561 agcaccgtcg aaggtgatcg cgatggccga ctgctggagt tcgctcggcg tcgagaaccg
     1621 tcccaggacg cgcatctgcg ctcccgggat caacgcctgc acttccgagg tgtccacgat
     1681 cagcatctgg ttgttggcgg catcggcgaa gtagccgcgc gtgccgtcgt ccgagatcga
     1741 gcaaccgtgg aataccgcgg gcaacgggtc cgcgggcagc cacgggacgg tcatctcgcg
     1801 cgggttcgcc ggatccgtga ggtcgatggg agtgatggtg ccggcggagc tgtaatagac
     1861 catgccgtcg ggctggaaac acccctcgtg cccggcttgc gtgggcaaga tgacgtcggc
     1921 gagcaagcgc gggtgggcac agtcctcgga cacgtcgtag acggcgagtg cgcgcatgat
     1981 ctccggcggc tcgatcccgc cgtcggccgt atacggcggg gggaacgcgt agtaattggc
     2041 gaccaggagc ccgcgctcgc ggttgacgcg cagcgactcg cccgggcttc gcatcgcgcg
     2101 ggcggtgagg tagtcggtct tcacgggatt gctcgggtcg gacacgtcga ggacgacgac
     2161 cccggcgccg atggcgacgc tgacgttgtc ggggtagtag acgcagtcgc cgtaggcgtc
     2221 gaaggtcgcg tggccgcga
//
LOCUS       DATLFO010000174        18687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_47084, whole genome shotgun sequence.
ACCESSION   DATLFO010000174 DATLFO010000000
VERSION     DATLFO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47084"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VM841_06950"
     CDS             <1..1298
                     /locus_tag="VM841_06950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVL89956.1"
                     /translation="PAATETRDDEAGVSTASPATAPASPVVAAPTRAPSPSPVQTVSS
                     ADDQAARALLDELGELLPGATLSGDTPMDRLAVAIEAVRVRLAEVERRLARWLAEEKL
                     SAERLAAAEQAVLDAGAAVAEAEEAVREQAGDFYMAPAWSEAIVFAAGNLEDAGRARG
                     YMTALLGDRVDVVHALRTAQVRLETERDAVHAASAALSKAGERIRAEHAVLNALTLKM
                     RALLDEVVRSYGAGSAEFARIIGEAQRGQSPAAVLERFVFAPVEGPVTSPFGPRIHPI
                     YGYRSLHTGTDIAATEGTALLAPRDGTVLAAGPAGPFGLSVVIDHGGRIATVLSHLSL
                     VSVAPGDVVRSGDVVGFAGCTGWCTGPHLHFEVWFDGRALDPADWMIERPLPGPLVVV
                     RGEDEGRLEPPEPPEPPEPAGAGTLTGLRNPFEDSAARS"
     gene            complement(1312..4713)
                     /locus_tag="VM841_06955"
     CDS             complement(1312..4713)
                     /locus_tag="VM841_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89957.1"
                     /translation="MAAVLVVSAFAVFPGGRQANLAADCPAGFTPVGAEERRLSQWGD
                     LLEVDLPQSLGCVNDKHPESLRELEALAAQRAAIQTAPNGITPAGAFQRAMNERRTML
                     ANGQSRANQFNWYPVGEGPLQAADAGYNSVNGLGNVELAARITTFDYIPAGDKFYPDT
                     LLASVSYGGVWMSDASARSWTSIGDSLPTQIVGAVAYTPANRGTILALTGDGSFGKDS
                     REGAGAYYTNDLGLTWKRATGVPDEAFGFKLAVNKADPRIVYAATGSGLYRSTDGGRS
                     YANVNLPTGECAGKSNRVRACLLANMVTDVVVQEPGGTTNANGHAVMAAVGWRGGMMK
                     NPDGTVQSPANGIYTSADGKPGSFTKSAATGFAPQERIGRLELGAAIGPNQNHNYVYA
                     MVQDAVLLRGGLPGIDAPGADRTVRVPTVFNGIYVTSDFGQTWRLVADQAALQHPATG
                     SALAVVAQGLSGYGPGVQAWYNMWLRPDPTRSIGGIPTRLVFGLEEVWQNELNVPITG
                     PTSFKVIGRYFSGETCMFLSGVPACPTNRGEALTHTTTTHPDQHDGLFIPDGNGGVTL
                     VAGNDGGAYAQHVGAGQEFSNNRWGVGNNRGFDTLLPYDAVRSADGTVWMGLQDNGTA
                     KIVDIKKNGRVIEKGRKIMTKGGDGFFVAVHPTNGNIAYGEYVGGAIAATTDGGKTWS
                     EMSPPITNAQFSNPFVMDPKDPDHLMIAGRQVVETGSGPGTSADDWAKVFDLGTAKKP
                     GDPTAVPAPDDPVNQMTAIDLNGSAAYVGFCGTCDVLNNRNPFKSGLATNVGGSKPPK
                     RFSSQGWHFAKAAGLPNRYITSIKIHPTNPKIVWVTLGGYARPWTPPGKLGAKNDGVG
                     YGHVFVSTDAGETFKDISANLPNVPVNWIEKRDREIIVATDIGVYISKPDVICEHYHW
                     RNCNKYEVLGNNLPLAPVYTIRLSHGDPNLLIAATYGRGVWSYRFGDPRQTAGSTGSK
                     PLPAPKFLGKTLAEFGFETGDEGWTAKSNSDTSSWRRGQPGHQSGFSFQNIPYGDAAS
                     VSLMSPKIALPERSTVKVSWYELRDTEPCCDFFTMDWSSDGYVWKPVRSIDGMNPEFP
                     EFGKTTVTFVAPKGELYLRFRVTSDQLVSFPPYSGVALDNVKIER"
     gene            complement(5039..5965)
                     /locus_tag="VM841_06960"
     CDS             complement(5039..5965)
                     /locus_tag="VM841_06960"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVL89958.1"
                     /translation="MSNRLLTHDALIYDSEPAFVETVVPFIEEGLAAGEGILVATSPG
                     NTALLREAMSDDGGRIVFADGSDVYQRPETAVAAYDSALRSLAAQGYRAVRAIGEVQH
                     GTGRLERWLPYEPVADRVFSDRPLWVVCPYDARTLPGWLIEHAKRTHSFIFGPQRERS
                     RTFVEPEQLMASLRPAPSDLVPNGPPLVQHAARGNLRELRSAIIANTKGAEPQVAERI
                     VTVVNELATNVIKHGGGTAYVSLWKVPAGYVADVVDPGGRLDDAFAGYRPPPNPATGG
                     VGLWLARQMSDEFTLLGGRGLTIRVGFESRPA"
     gene            complement(5967..6842)
                     /locus_tag="VM841_06965"
     CDS             complement(5967..6842)
                     /locus_tag="VM841_06965"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HVL89959.1"
                     /translation="MKRAMDHQDPDRIVDLREARLGDHICVGYDDDRFFRRTATEFFA
                     AGIEASERVGFFAPPPKLAELTAWLSASGIDVNRLASRGQIVLGDVSAAYLPDGAFDP
                     KRRIESFSASIDEALADGFTGFRVIGDVASIMDRQDVRAEWLSYELRADLLAARKPIA
                     ALCSFDHRVCPEQVALEAAAVHSYRYGDVAEPPPFRIVAGAGAGLRIEGEIDFGSATS
                     VAKLLLNASEDAAPAIDVSDLAFIDAAGMRALASGAVALLQRHPGVQIRGGSQIFKRM
                     WDLMGLDRFETIAVR"
     gene            6926..7669
                     /locus_tag="VM841_06970"
     CDS             6926..7669
                     /locus_tag="VM841_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89960.1"
                     /translation="MKLRIAGLSVGSLSVLAAAILFDARVALANHSPYHYALEWTSVV
                     QGFYRHDSAGPLVDAYFGWRGISGRIIAPETVPAMNDCRIDHENGAVVAYHARGTIRS
                     VLIAIGWYTGTLGDCSLECIRRGGSPSTDCWRVWLNGSVRASQCALQFPTTAAMLAGT
                     EILNRSKQENPQAPSINMPLSRYGSANLSETSSLKVLGVNGWEPWDTSLTAGFTSRYD
                     ESTGQENGIAPRYVISVDNAFYNFVTYRQ"
     gene            7680..8867
                     /locus_tag="VM841_06975"
     CDS             7680..8867
                     /locus_tag="VM841_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89961.1"
                     /translation="MMPSSAKAAARPAQWMLGLIVSMIVVTSSCGRSIERQSLTEARW
                     TPVSIEIHQGLRQAVYDPRRDSIWILTAPADPRLPALEEKLVELTEYRITTRETIQHR
                     SPLTSRTLGIGNAMAIDANGDLWMAWDQNLVRFRPSDGSFESWEIAPTVLADPEEGST
                     ARMAVALAIDQSSAVWVTVDGAPSAYRFVVDETRWEVISLGDVVTTTEKRITVTSSHG
                     IAVNGIAGGSSSAAPKLALLDSRDWKPRSGKVAARQIAPLNETAIAYTDDRDRVGAAN
                     STDGRSIYSRAIGKVPPRALTTHGERIWVWVMEEGRVVVHRMDPRNDQMDRVTFPHRE
                     GFGRGSFSDYRGDAPATQFSIADPDIQEMIVDKRGVAWLVTSHAGVTSDRGGYEPLYR
                     LMI"
     gene            complement(9057..9692)
                     /locus_tag="VM841_06980"
     CDS             complement(9057..9692)
                     /locus_tag="VM841_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89962.1"
                     /translation="MLNASTSPGEFENDAELTDRILVQELQLTWNATRALWLATERAI
                     AFGLTIIGAASAAGVRDHLTEVKLFLPVALALIYGYGLNMMHEMMGIASYRGRLERIA
                     RMRGDLDFAMWESGIAAKAIVFRAPQLAAFAIPALLYLAATVVSLVTAIRYSGSYTRW
                     VNWLVPILDAALLIILLIALVSVMGVGARVQAAIIGDEARIDSDSGEKDHE"
     gene            9749..10069
                     /locus_tag="VM841_06985"
     CDS             9749..10069
                     /locus_tag="VM841_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVL89963.1"
                     /translation="MARNSRVPPEVRQRACRLVLDSVEEHGSRWAATKSVAAKMGFHP
                     ETLRAWVKQFEIDNGKTPGLTTDERTRLKQLERENFELRRANEILKSASAFFAAELDR
                     PQKR"
     misc_feature    10027..10140
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            10066..10839
                     /locus_tag="VM841_06990"
     CDS             10066..10839
                     /locus_tag="VM841_06990"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HVL89964.1"
                     /translation="MIAYVDANKERFGVEPICAQLPIAPSTYYDAKNRAPSKRAVRDE
                     YLKVEISRVWRENNKGVYGAPKVWRQLNREGIRAANCTVRRLMRDLGISGVRRGRYCR
                     TTISDESLQRAADLVNRTFKAARPNQLWVADITYVATMENRFVYVAFVVDVFSRMIVG
                     WRASNSLRSDLALDALEMAIWQRQPALNALIHHSDRGSQGGINWSSQHLDSGGVGWQR
                     CGSGSGRRSCIEDRFRRRDVQRWRGVRTGLHSGQRSHAG"
     gene            10914..12113
                     /locus_tag="VM841_06995"
     CDS             10914..12113
                     /locus_tag="VM841_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HVL89965.1"
                     /translation="MNPCLPATVSGRYLSSSEREDIALLRAQRLCVREIARRLGRSPS
                     TISRELRRNASLRTWRMEYKASIAQWHAERRARRPKKAKLATNEQLRDYVQDRLSGLV
                     HRPDGHAVGPGVTRERKGRSKPHRADRRWVQGWSPEQISKRLRIDFPDDESMRISHEA
                     IYQALYVQGRGALKRELVACLRTGRALRVPRARARQKTWAHVTPEVMISERPAEVEDR
                     AVPGHWEGDLLIGLHRSAIGTLVERTTRFTMLIHLPREVGYGVVPRTKNGPALAGYGA
                     VTMKNALASVMTTLPEHLRRSLTWDRGKELSQHAAFKVETGIPVFFADPQSPWQRGTN
                     ENTNGLLRQYFPKGTDLSRWTADEIDAVADALNRRPRKTLGWKTPAEAFDEHVKSVEQ
                     GCVATTR"
     gene            12121..12465
                     /locus_tag="VM841_07000"
                     /pseudo
     CDS             12121..12465
                     /locus_tag="VM841_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(12491..12886)
                     /locus_tag="VM841_07005"
     CDS             complement(12491..12886)
                     /locus_tag="VM841_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89966.1"
                     /translation="MGVKQFRTLVSEFGDPCNLTFLFALARPSQLGCKGEEPGADLFD
                     PVGFELDRLVVVGNGAFDFVGQDVLRRAGFAAAAPEAEEVEVASLGVGEAESGSAPAA
                     EDGAFEVNRDGFIGDSILTIASLRDGSIQ"
     gene            complement(12914..13072)
                     /locus_tag="VM841_07010"
     CDS             complement(12914..13072)
                     /locus_tag="VM841_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89967.1"
                     /translation="MIGGFDVGAIAVSGVTECDVEVLLDPLEINGNIFENGFGFRDFA
                     VDPILLAL"
     gene            complement(13257..13333)
                     /locus_tag="VM841_07015"
     tRNA            complement(13257..13333)
                     /locus_tag="VM841_07015"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13297..13299),aa:Glu,seq:ttc)
     gene            13445..13849
                     /locus_tag="VM841_07020"
     CDS             13445..13849
                     /locus_tag="VM841_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007084407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HVL89968.1"
                     /translation="MSSHPSVVAKVRVGKSRGKGRGVFATERIAGGEVIERSPVIPIP
                     KRHWKHIEKTILGDYAFDWGRDLEDVAIVLGYGMIYNHSPKPNARFDFKLREQVYEFI
                     ALRDIEPGEEIFTNYNGDPKDLTPLWFEKRKA"
     gene            14083..15792
                     /locus_tag="VM841_07025"
     CDS             14083..15792
                     /locus_tag="VM841_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89969.1"
                     /translation="MRLSDRGRAAAGPAIALVLVASTLVTAAPASPSSSYLVVWAGDW
                     NAGDARAGAPDLAIEAGADLAAGPDFLAVVDADPSSASYGKVVRTLPVSLLENEPHHM
                     QYAWSPGDRIFAGGLFSDRTFVFDVDSIPDVRLEGVVEPLDTPCGSVPDAYWVLSDGT
                     AYGTYMGGPNVAGDPRCNGGISNGFAGTPGSIVRISPDAKVLSETSAAGAFNQPHPND
                     AGRPAQCVSNPPLARPSCANPHGIQVREDLHVMITSDYAEPRNVPIDPVSAPDPNVFR
                     DTVRVWDIRDRNRPVLHSVSVMPRGSMAFDPPPQVDARGVMGIMETTVTNVPQHRGAF
                     AQSMCGGQIYYSADITVKNPMWRQVFDVSVAANALDPDVGRVQGCTGGGWLQTSPDDT
                     TLFHTVIGRNARTLGPSDPGVPKMLIAIDIRSLVAIPPSQWTLDSTTICTIDTAAEVV
                     AGGSEPSCPSVAGVVELADATTGGPHWGAIDNFSLSSAPGRLTSVTRIAVSNYFVGRS
                     GLDGNHGLCMVDVAAGGSLSIDGGFRDEHTGDACVSFDRPIWPHGTYGRAKPHSMLFV
                     TPP"
     gene            15862..17301
                     /locus_tag="VM841_07030"
     CDS             15862..17301
                     /locus_tag="VM841_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89970.1"
                     /translation="MSALVRSLRRAPAIAIVVGLMMSITATGPASASRFGKAGPPQFP
                     SFPYEERLGSGGPPPFIQDADDPHKWVWTIDPSKYAATGTITFWDYSNAITREGFMSG
                     PGGASVNDFQVTSPVTGTKGFDFERPSFTQDVDSVEADFLTPDPPHDIHTDFDPTAYH
                     PDEFPMPTPFATWTGANIDGVANLYKFAYSVAPGTQFMNMQVDKAGNYYVARRDMQWE
                     WIDELVFKNSDGDIQREDTQFNIQPYPISDAYGWCGALVMRDPSSLSSMAGQLIKDIA
                     FDVFAADTSPASAIAPVAREVVPSFVMRSFGIYEVDVTMLSTGVRQQFTGIAKGVHIR
                     PVAVTIGSVTYPAGSRVEPGDPDFSHWLNRVSPFAAGVVPIGAWVINEGTPYMQVVPA
                     GTPGAVWHQNKFAGAAFLVRGDMERRVNYINTASGLAADQATVTGYNEVYHSLIQLGL
                     TNSKTGQSWKRADWADWEVGGTVPVANGY"
     gene            complement(17353..17847)
                     /locus_tag="VM841_07035"
     CDS             complement(17353..17847)
                     /locus_tag="VM841_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89971.1"
                     /translation="MSRGFRRVIAAHAIGVALIACGGDEPRPVASPGEVPNERVSTSN
                     ALPREFPAGFPLPPKRTVMYSAVSPIGAVVYFTSELRGEALTTMLRTQLPDKGWKLHS
                     CVRSEGGPEPLYTVVASRGRTVATAVVGYAPEAASRIQGRVYTFFVSVATDAAPPVSG
                     SDEC"
     gene            complement(17886..17957)
                     /locus_tag="VM841_07040"
     tRNA            complement(17886..17957)
                     /locus_tag="VM841_07040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17922..17924),aa:Lys,seq:ttt)
     gene            complement(18363..>18687)
                     /locus_tag="VM841_07045"
     CDS             complement(18363..>18687)
                     /locus_tag="VM841_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL89972.1"
                     /translation="INHEHYKRRIRSVAVWESLSNTARNHSCAMSRSDRLHHNPNLTR
                     QVRGWRIIGENVAVGWDLYEIHKAWMASPSHRDNILERRYRALGVGICRGPTGAYWVT
                     TIFYA"
BASE COUNT         3260 a         6088 c         6123 g         3216 t
ORIGIN      
        1 ctcctgcggc gacggagacc cgcgacgacg aggcgggcgt ttcgaccgca tcgccggcga
       61 cggccccggc ctcgcccgtg gtcgctgcgc cgacgcgagc gccctcgccg tctccggtgc
      121 agacggtttc ctctgccgac gatcaggccg cgcgcgcgtt gctcgacgag ctgggagagc
      181 tcttgcccgg cgcgactctc tcgggagaca ccccgatgga ccgcctcgcc gtggcgatcg
      241 aagcggtccg cgtccggctc gccgaggtcg agcggcggct cgcgcgatgg ctggcggaag
      301 agaagctctc cgccgagcgg ctcgccgccg ccgagcaggc cgtcctggac gcaggcgccg
      361 ctgtcgccga agccgaggaa gccgtgcgtg agcaggccgg ggacttctat atggcgcccg
      421 cgtggagcga agcgatcgtg ttcgctgcgg gcaacctcga ggacgccggc cgcgcccgcg
      481 gttacatgac ggcgctgttg ggtgaccgcg tcgacgtggt ccacgcgctg cgaaccgctc
      541 aggttcgtct agaaaccgag cgcgatgccg tccacgccgc gtcggcggcg ctgtcgaagg
      601 ccggtgagcg catccgcgcc gagcatgccg tcctgaacgc attgaccctg aagatgcgcg
      661 ccttgctcga cgaggtcgtc cgctcctacg gagccggttc ggcggagttc gcgcgcatca
      721 tcggcgaggc gcaacgcggg cagtcgccgg cggcggtcct ggagcggttc gtgttcgcgc
      781 cggtcgaggg gccggtcacc tcgccgttcg ggccgcgcat ccatccgatc tacggctacc
      841 ggtcgctcca caccggaacc gacatcgccg ctacggaagg cacggctctt ctcgcacccc
      901 gggacggtac cgtgctcgcc gccgggccgg ccgggccgtt cggtctgtcg gtcgtgatcg
      961 accacggcgg gcggatcgcg acggttcttt cgcatctttc gctcgtgtcc gtcgcccccg
     1021 gtgacgtggt tcgctcgggt gacgtcgtcg gcttcgccgg gtgcaccggc tggtgcaccg
     1081 gcccgcacct tcatttcgag gtctggttcg acggacgcgc gctggatccc gcggactgga
     1141 tgatcgagcg gccccttccc ggaccgctcg tcgtcgtccg cggcgaggac gaaggccggc
     1201 tcgagccgcc cgagccgccc gaaccgcccg aaccggcggg ggccggaacg ctgaccgggc
     1261 tgcgaaatcc gttcgaggac tccgcagccc ggtcgtagtg cgtttcggtg gctaccgttc
     1321 gatcttgacg ttgtcgagcg ccaccccgct gtagggcggg aacgacacga gctgatcgct
     1381 ggtcacccgg aaccgcaggt acagttcgcc cttcggtgcc acgaacgtga ccgtggtctt
     1441 gccgaactcg gggaactctg ggttcatccc gtcgatggac cgaacaggct tccagacgta
     1501 gccgtcgctc gaccagtcca tcgtgaagaa gtcgcagcac ggctcggtgt cgcggagctc
     1561 ataccagctg accttcacgg tgctgcgctc gggcaatgcg atcttcggcg acatcagcga
     1621 cacgctcgcg gcgtccccgt agggaatgtt ctggaagctg aagcccgact ggtggccggg
     1681 ctggccgcga cgccacgagc tcgtgtcgct gttcgacttc gccgtccatc cttcgtcgcc
     1741 ggtctcgaag ccgaactcgg cgagcgtctt gccgagaaac ttcggagccg gaagcggctt
     1801 gctccccgtg ctgccggccg tctgacgcgg gtcgccgaac cggtagctcc acacgccgcg
     1861 gccgtacgtg gccgcgatga gcaggttcgg gtcgccgtgc gaaagacgaa tcgtgtacac
     1921 gggcgcgagc ggcaagttgt tgccgagcac ctcgtacttg ttgcagttgc gccagtggta
     1981 gtgctcgcag attacgtcgg gcttcgagat gtagacaccg atgtcggtgg cgacgatgat
     2041 ctcgcggtcc cgcttctcga tccagttcac cggcacgttc ggcaggttcg ccgagatgtc
     2101 cttgaacgtc tcaccggcgt ccgtcgaaac gaacacgtgg ccgtagccca cgccgtcgtt
     2161 cttcgcgccg agcttgcccg gcggcgtcca cggacgcgcg tagccgccca gcgtgaccca
     2221 gacgatcttc gggttcgtcg ggtggatctt gatcgaggtg atgtagcggt tgggaaggcc
     2281 tgccgccttc gcgaagtgcc agccctgcga actgaagcgc ttcggcggct tcgatcctcc
     2341 gacattggtc gccaggccgc ttttgaacgg gttgcggttg ttgagcacgt cgcaggtgcc
     2401 gcagaagccg acgtaggccg ccgagccgtt caggtcgatc gcggtcatct ggttgaccgg
     2461 gtcgtccggc gcgggcaccg ccgtcggatc gcccggcttc ttggccgtcc ccaggtcgaa
     2521 gaccttcgcc cagtcgtcgg cggacgttcc cgggcccgag cccgtctcga cgacctgacg
     2581 cccggcgatc atcagatgat cgggatcctt ggggtccatc acgaacgggt tcgagaactg
     2641 cgcattcgtg atcggcggcg acatctcgct ccacgttttt ccgccgtcgg tcgtggccgc
     2701 gatcgcgccg ccgacgtact cgccgtaggc gatgttgccg ttcgtcgggt gcacggcgac
     2761 gaagaagccg tcgccgcctt tggtcatgat cttgcggccc ttctcgatga cgcggccgtt
     2821 cttcttgatg tccacgatct tcgcggtgcc gttgtcctgc aagcccatcc aaaccgtgcc
     2881 gtccgccgag cgcactgcgt cgtagggcag caacgtgtcg aagccgcggt tgttgccgac
     2941 gccccagcgg ttgttcgaga actcctggcc cgccccgaca tgctgcgcgt aggcgccgcc
     3001 gtcattgccg gccacgagcg tcacgccgcc gttgccgtcg gggatgaaaa ggccgtcgtg
     3061 ctggtcgggg tgcgtcgtcg tggtgtgggt gagcgcctcg ccgcggttgg tcgggcatgc
     3121 gggcacgccg gacaggaaca tgcacgtctc gcccgagaag tagcggccga tcaccttgaa
     3181 cgaggtcggc ccggtgatcg ggacgttgag ctcgttctgc cagacctctt cgagcccgaa
     3241 cacgagccgg gtcgggatgc ccccgatcga acgcgtcggg tcgggacgca gccacatgtt
     3301 gtaccaagcc tgcacgccgg ggccgtagcc ggacaggcct tgcgcgacga ccgcgagtgc
     3361 cgagcccgtc gccggatgct gcaaggccgc ctggtcggcc acgagccgcc aggtctgacc
     3421 gaagtcgctc gtgacgtaga tgccgttgaa gacggtgggc acgcgaaccg ttcgatccgc
     3481 gcccggcgcg tcgatgccgg gcagcccgcc ccgcagcagc acggcgtcct gcaccatcgc
     3541 gtagacgtag ttgtggttct gattcgggcc gatggccgcg ccgagctcga gccggccgat
     3601 gcgctcctgc ggcgcgaatc ccgttgcggc gctcttggtg aacgatcccg gcttgccgtc
     3661 ggccgaggtg tagatgccgt tcgccggcga ctggaccgta ccgtcggggt tcttcatcat
     3721 gccgccgcgc cagccgaccg ccgccatgac cgcgtgaccg ttggcgttgg tggtgccacc
     3781 cggttcctgc accacgacgt cggtcaccat gttggccagc aagcatgcgc ggacgcggtt
     3841 cgacttaccc gcgcactcgc cggtcggcag gttgacgttg gcgtaggaac ggccgccgtc
     3901 cgtggagcgg tacaggcccg agccggtcgc tgcgtagacg atgcgggggt cggccttgtt
     3961 gaccgcgagc ttgaagccga acgcctcgtc cggcacgccc gtggcgcgct tccaggtcag
     4021 accgagatcg ttcgtgtagt acgcgcctgc gccttcacgg ctgtccttcc cgaacgaccc
     4081 gtccccggtg agcgcgagga tcgtgccgcg gttcgccggc gtgtacgcga cggcaccgac
     4141 gatctgcgtc ggcagcgagt cgccgatcga ggtccacgac cgcgcggacg cgtcgctcat
     4201 ccacacgccg ccgtaggaaa ccgacgcgag cagcgtgtcc gggtagaact tgtcgccggc
     4261 cgggatgtag tcgaacgtgg tgatgcgcgc ggcgagctcg acgttgccaa gcccgttcac
     4321 cgagttatag ccggcatcgg ccgcctgcag cgggccttcg ccgaccggat accagttgaa
     4381 ctgattcgcg cggctctggc cgttcgccag catggtgcga cgctcgttca tggcgcgctg
     4441 aaaagcgcct gcgggcgtga tgccgttcgg cgcggtctgg atggcggcgc gctgggccgc
     4501 gagcgcctcg agctcgcgca gcgactcggg atgcttgtcg ttcacgcatc cgagcgactg
     4561 tggaagatca acctcgagca ggtcgcccca ttgcgacagg cggcgctcct ccgctccgac
     4621 cggcgtgaag cccgccggac agtccgccgc gaggttggct tgccgtcctc ctggaaagac
     4681 cgcgaatgca gagacgacga gcaccgctgc catgggaagc gacgctcgac gcaaaagaga
     4741 gaatttgctc acgattatcc ccaccgttca cccgggtcgc gtgcgaccgt tttcgaaatc
     4801 aacgaccgat ctcgtgattc ggtcgtgccg ggacgggatc ggccccgccg tttcgggaac
     4861 attcatcacg gttcctgtcc gatgaacggc cgtctggtcc tgcccgcaat cgaacacgtc
     4921 gtgccgttga gcggcctttc tagcattttc gcgatgcgcg aacaaacgac gggaatgcaa
     4981 gcagccgttg gctgtcggcc gtgaagggga ggaagcgaat gaatcgtgag cttcgcgact
     5041 atgcggggcg cgattcgaag ccgactcgaa tcgtgaggcc tcggccgccg agcaaggtga
     5101 actcgtccga catctgacgc gcgagccaca gacccacgcc gcctgtggcc gggttcggcg
     5161 ggggacggta gccggcaaat gcatcgtcca ggcggccgcc cggatccaca acgtcggcca
     5221 cgtatccggc cggaaccttc cacagcgata cgtacgccgt cccgccgccg tgcttgatca
     5281 cgttggttgc aagctcgttc acgacggtga cgatgcgctc ggcgacctgc ggctcggccc
     5341 ctttggtgtt cgcgatgatc gccgagcgca gctcgcgcag gttgccgcgg gctgcgtgct
     5401 gcaccagggg cgggccgttc ggcaccagat ccgacggcgc agggcgaagc gaggccatca
     5461 gctgctccgg ctcgacgaac gtccggcttc gctctcgctg tgggccgaag atgaaggagt
     5521 gggtacgttt tgcgtgctcg atgagccatc caggcagggt tcgtgcgtcg tacgggcaga
     5581 cgacccagag cggccggtcg gaaaagacgc ggtcggcgac cggctcgtag gggagccacc
     5641 gctcgagtcg gccggtgccg tgctgcacct cgccgatggc gcgcaccgcc cggtaaccct
     5701 gggcggcgag cgagcggagc gccgagtcgt aggccgcgac ggccgtctcc ggccgttgat
     5761 agacatctga gccgtcggca aagacgatcc ggccgccgtc gtcgctcatc gcctctcgta
     5821 gcaaggccgt gttgccggga gacgtggcga cgaggatgcc ctcgcctgcc gcgaggccct
     5881 cttcgatgaa cggaacgacc gtttcgacga acgccggctc cgagtcgtag atgagagcgt
     5941 cgtgggtgag cagacggttg ctcatgtcat ctcaccgcga tcgtctcgaa ccggtcgagc
     6001 cccatgaggt cccacattcg cttgaagatc tgcgatccac cgcggatctg gacgccggga
     6061 tgtcgctgaa gcaatgcgac ggccccgctc gcaagggcgc gcatgcccgc cgcgtcgatg
     6121 aacgccagat ccgacacgtc gatcgccgga gccgcgtcct cgctcgcatt gagcaatagc
     6181 ttggccacgg aggtggcgga gccgaaatcg atctcgcctt cgatgcgcag gccggccccg
     6241 gcgccggcga cgatgcggaa cggcggcggc tcggcgacgt cgccgtaccg atacgagtgc
     6301 acggcggccg cttcgagggc gacctgctcg ggacacacgc ggtgatcgaa ggaacacagc
     6361 gccgctatgg gcttgcgcgc ggcgagaaga tcggcccgca gctcgtagga gagccattcg
     6421 gcccgtacgt cctgccggtc catgatcgag gcgacgtcgc cgatgacgcg aaagccggtg
     6481 aacccatcgg ccagcgcttc gtcgatgctc gccgagaagc tttcgatccg gcgcttcggg
     6541 tcaaacgccc cgtccggcag ataagccgcc gagacgtcgc cgagaacgat ctgtccccgc
     6601 gaagcaagcc ggttcacgtc gatgccggag gccgaaagcc acgcggtgag ctcggccagc
     6661 ttcggcggag gagcgaagaa cccgactcgc tcgctggctt cgatcccagc agcgaagaac
     6721 tcggtggcgg tacggcggaa gaaacgatca tcgtcgtagc cgacgcagat gtgatcaccg
     6781 aggcgagcct cgcgaagatc gacgatcctg tccgggtctt gatggtccat ggctcgcttc
     6841 acacccctcg aagtattcgt atctaccccg ttgggagtgc cccaaacgct ttcgggccgc
     6901 gagcatgcgg actggggaga acacgatgaa gcttcgtatt gccggcctat ccgttgggtc
     6961 gctatctgtt cttgctgcgg ccattctttt tgacgcacgt gtcgctctgg ctaatcactc
     7021 gccttatcat tacgccttgg aatggacgtc agtagtgcaa ggtttctacc gccatgattc
     7081 ggctgggccg ctggtcgatg catactttgg gtggcgtgga atctccgggc gcatcatcgc
     7141 gccggagacg gtgccggcaa tgaacgactg ccgcatagac catgaaaacg gcgccgtggt
     7201 cgcataccac gcgcgaggca cgatccgctc ggtgctaatc gcgatcggct ggtacacagg
     7261 gacactcggt gattgctcgt tggagtgcat tcggcgcggc gggagtcctt cgactgactg
     7321 ctggcgagtc tggctaaacg gttcagtccg cgcgagtcag tgcgcacttc agtttccgac
     7381 aaccgctgcg atgctggcag gaaccgaaat tctcaatcgc tccaagcaag agaatcctca
     7441 ggctccttcc ataaacatgc cgctatcacg atatggctcc gcgaacttat cggagacaag
     7501 ctctctcaag gttctcggcg ttaacggctg ggagccctgg gatacgtcct tgacggccgg
     7561 cttcacgagc cgatacgacg aatcgaccgg tcaggaaaac gggattgcac ctcgatatgt
     7621 catatcggtc gataacgcgt tctacaattt cgtcacctac cgccagtagg gggtgctcca
     7681 tgatgccttc gagtgcgaaa gctgctgccc ggccggcgca atggatgctg ggactaattg
     7741 tgtcgatgat tgtggtaacc agctcctgcg ggcgcagtat cgagcgacaa tcactcaccg
     7801 aagcgcgatg gacccctgtc tccatcgaga ttcaccaggg cctccgccaa gccgtgtatg
     7861 accctcgtcg ggactcgatc tggattctca cagccccggc agatcctcgt cttccagcgc
     7921 ttgaggaaaa gctcgtcgag ctcaccgagt accggatcac cactcgagag acgatccagc
     7981 accggagccc gcttacgtcc cggacattgg gaattggcaa tgccatggcc atcgacgcaa
     8041 atggcgacct ctggatggca tgggaccaga acctggttcg atttcgaccg tccgacgggt
     8101 cgtttgagtc ctgggagatc gctccgacgg ttcttgccga tccggaggaa ggctcaacgg
     8161 cgaggatggc tgtcgcgctc gcaatcgatc aatcgagtgc ggtctgggtc actgtcgatg
     8221 gggctccttc ggcttaccgc ttcgtcgtgg atgagacgcg gtgggaagtg atttctctcg
     8281 gcgacgtcgt gaccaccacc gagaaacgca tcacagttac ctcctcccac ggaattgcgg
     8341 tcaacggcat cgctggcggc tcctcgtcgg cggcgcccaa gctcgcgctg cttgattcgc
     8401 gagactggaa gccacgatcc ggaaaggttg ccgctcggca aatcgctccg ctcaatgaga
     8461 ccgcaatagc ctataccgac gatcgagatc gagtcggagc agcgaactcc accgatggcc
     8521 ggtccatcta ctcgcgggcg atcggaaagg tgccgccccg cgcgctcacc acgcatgggg
     8581 aacggatctg ggtgtgggtt atggaagagg gacgtgtcgt cgttcatcgc atggacccgc
     8641 gaaatgacca aatggacaga gtgactttcc ctcaccggga aggtttcggc aggggctcgt
     8701 tctccgatta ccgcggagac gcaccagcca cgcaattctc gatcgccgac ccggatattc
     8761 aggagatgat tgtcgataag agaggggttg cctggctcgt tacatcccat gcgggcgtca
     8821 ccagcgatcg tggaggctac gaaccgctct accgtctgat gatttagtcc cacatcggcc
     8881 tgggcgcgcc ctggtcaaac ccaccgccac gacggcagcg atcgcgctgt ggccgcgctg
     8941 tcatcgctcg atgatatcca atcgacatct caaagactca cgctaaacat cggtgctcca
     9001 gcacccggcg agcctgcaaa aaaccaggcg aagcggactc tcaaagaggc ctcactctac
     9061 tcatgatctt tttcgccaga gtcagaatcg atccttgctt cgtcacctat gatcgccgct
     9121 tgaacacgag cgcccacgcc catgacgctc accagcgcga tcaagaggat gatcagcaat
     9181 gccgcatcta gtatcggcac cagccagttc acccacctcg tatagctccc gctgtagcga
     9241 atcgcggtaa cgagactgac aacggtcgcg gcgaggtaga gcagcgcggg aatggcgaag
     9301 gcggccagtt gtggagcacg aaagacaatc gccttggctg cgattccact ctcccacatc
     9361 gcgaagtcga ggtcgccgcg catccgtgcg atccgttcga gccttcctct gtacgaggca
     9421 attcccatca tctcgtgcat catgttcagc ccgtacccgt agatcagcgc gagcgcgaca
     9481 ggaaggaaca acttgacctc cgtcaaatgg tcacgaactc ctgccgcaga tgctgcgcca
     9541 atgatcgtaa ggccgaaggc gatagcacgc tcagtcgcca gccatagtgc cctggttgcg
     9601 ttccaagtaa gttgaagttc ctgtacgagg atgcgatccg tgagttcagc gtcgttctca
     9661 aattctccgg gacttgtgga tgcgttaagc acctgaactg aatcgcgacg ggtaatccgg
     9721 aggctccaat ccgtggaagg atggggccat ggccaggaac agcagggtac ctccggaggt
     9781 tcgtcaacgg gcctgcaggc tcgtgttgga cagtgtcgag gagcacggat cgagatgggc
     9841 ggcgaccaag tcggtcgcgg cgaagatggg ctttcaccct gagacgctgc gcgcatgggt
     9901 caagcagttc gagatcgaca acggcaagac gccgggtctg acgaccgacg agcgcacgcg
     9961 tttgaagcag ctcgagcgag agaacttcga gctgcgtcgc gcgaatgaga tcttgaagtc
    10021 ggcatcggct tttttcgcgg cggagctcga ccgcccacaa aagcgatgat cgcctacgtc
    10081 gatgcgaaca aggaacggtt cggggtcgag ccgatctgcg cgcaactgcc gatcgccccg
    10141 tccacttact acgatgccaa gaaccgggcg ccgtcgaaac gtgcggtccg cgatgagtat
    10201 ttgaaggtcg agatcagccg cgtctggcgt gagaacaaca agggcgtcta cggcgcgccg
    10261 aaggtctggc ggcagctcaa ccgggaaggg atccgcgcgg cgaactgcac cgtccgccgg
    10321 ctgatgcgtg acctggggat cagtggggtg cgccgcggcc ggtattgtcg cacgacgatc
    10381 agcgacgaat cgctgcaacg cgccgcagat ctggtcaacc gcaccttcaa agcggcgcgt
    10441 ccgaaccagc tctgggttgc cgacatcacc tacgtcgcga cgatggaaaa tcggttcgtc
    10501 tacgtggcct tcgtcgtcga tgtgttcagc cgcatgatcg tcgggtggcg cgcttcgaac
    10561 tcgcttcgca gcgatcttgc cctcgacgcg ctcgagatgg cgatctggca acgtcagcct
    10621 gctctgaacg cgttgattca tcactccgac aggggcagtc aaggcggaat caactggtcg
    10681 tcgcaacacc ttgatagtgg aggtgttgga tggcaacgat gcgggagcgg cagcgggcgg
    10741 cgcagctgta tcgaggacag attccgtcgc cgggacgtcc aacggtggcg tggcgtgagg
    10801 accgggttgc attctgggca gcgatcgcac gcgggctgaa gacggaggag gcggctgcgg
    10861 aggcgcgtgt ctcgcctccc gtcgcttttc gctggtttcg gcatgctggc ggcatgaatc
    10921 cgtgtctgcc tgcaaccgtc tcgggtcgtt atctatccag ttccgagcgt gaagatatcg
    10981 cgctgctgcg cgcgcaacgt ctttgcgttc gggaaatcgc tcgacgcctg ggtcggagtc
    11041 cgtcgacgat atctcgagaa ctgcgtcgca acgcttcgct gcgaacctgg cgcatggaat
    11101 acaaggcgtc gattgctcaa tggcatgccg agcgccgggc tcgccggcct aagaaggcaa
    11161 agctggccac caacgagcag ctgcgcgact atgtgcaaga ccggctgtcg ggcttggtgc
    11221 accgccccga cggtcacgcc gttggccccg gtgtgacgcg ggaacggaag ggtcgcagca
    11281 agccgcatcg cgctgaccgc cgatgggtgc aggggtggag cccggagcag atctccaaac
    11341 gcctccggat cgacttccct gatgatgagt ccatgcggat cagccacgaa gcgatctacc
    11401 aggcgcttta cgtgcaaggc cgtggtgcgc tgaagcgaga gctcgtcgcg tgtctgcgca
    11461 ccggacgagc cttgcgcgtt ccccgagcgc gcgctcggca gaagacgtgg gcgcatgtca
    11521 cgcctgaagt gatgatcagt gagcgtcccg ccgaagtcga agaccgagcg gttcccgggc
    11581 actgggaagg cgatctgctc atcggattgc atcgctccgc gatcggtacg ctcgtggaac
    11641 gcacaacccg gttcacgatg ctgatccatc tcccacgaga agttggatac ggcgtcgtgc
    11701 cgcgaaccaa gaacgggccg gcgctcgcag gctacggcgc ggtcacgatg aagaacgctc
    11761 tcgcgtcggt aatgacgacg ctgccagagc atttgcgccg atccctcaca tgggatcggg
    11821 gcaaggaact ctcccagcac gcagcgttca aggtcgagac cggaatccca gtcttcttcg
    11881 ctgatccgca gagcccgtgg cagcgcggca cgaacgaaaa caccaacgga ctgctgcgcc
    11941 aatattttcc gaaaggcact gacctctccc gttggaccgc cgacgagata gacgcagtcg
    12001 ccgatgcact caaccggcgg ccgcgaaaga cactcggatg gaagacgcca gcagaagcct
    12061 tcgatgaaca cgtaaagtcc gtagaacaag gctgtgttgc aacgacccgt tgaacctggg
    12121 caatatttgt cgatccgcta caccgaacgg ctcgccgacg ccggcatcga accctccgtc
    12181 ggctccgtcg gcgacagcta cgataatgcg ctcgctgaga cgatcatcgg cctctacaag
    12241 accgagctca tccaccacca gggtccgtgg cgcaacctcg attacgtcga attcgccaca
    12301 ctggaataca tcgactggtt caataatcgc cggctcctcg gctcgatcgg caatatccca
    12361 cccgacgaat atgaagccgc atggcgggcc gaaaacaaag aagcggcgcc gagcggcgcc
    12421 gcaccccagc aactgatcct gaccgacatg ctccccacac cgtaaactca ttcgacgaag
    12481 gaggctgaca tcactggatc gagccatcac gcaaggatgc gatcgtaaga atcgagtctc
    12541 cgataaaccc gtcgcgattc acctcaaagg caccgtcttc tgcggccggt gcggagcccg
    12601 actctgcctc accaacgcca agggacgcta cctctacttc ttctgcctcg ggcgcagccg
    12661 cggcgaaccc tgcccgacga agtacgtcct ggccgacaaa gtcgaacgcg ccgttgccga
    12721 ctactaccag acgatccagc tcgaacccga ccgggtcgaa aagatccgca ccgggctcct
    12781 cgcctttgca gccaagctgc gacggacgag cgagcgcgaa gagaaacgtc agattgcacg
    12841 gatcgccaaa ctcagagacc aacgtacgaa actgcttcac gcccactacg gcggcgctgt
    12901 agcgctcgat ctcttaaaga gcgagcagga tcggatcaac cgcgaagtcg cggaagccga
    12961 agccgttctc gaaaatattt ccgttgatct cgagcgggtc gaggagaacc tcgacatcgc
    13021 actcggttac gccggagact gctatcgcgc ctacatcgaa tcccccgatc acgtccggcg
    13081 acagttcaac cagtcgttct tcgagagggt gctctgggac gggctcaggg tcgtcggcgc
    13141 gcgactggca cagcccttcg cggaactact ctccgacgac ctcgtaaaaa tgctagagaa
    13201 ggaactgaag aaccccggcc ctgttctctt tgaccgcggt tcgaataaga accatttggt
    13261 gcccccagcg ggattcgaac ccgcgatctc cgccgtgaaa gggcggcatc ctaggccgct
    13321 agacgatggg ggcctgacgc ggattctacc cggcggcggc gcggggcgtc cgggcgcctg
    13381 ccctgtgcgg gcgccagcgc gtaccctatg gcctttcgaa accgtcgaag gagagtcgtc
    13441 accggtgagc agccacccca gcgtcgtcgc aaaggtccgg gtcgggaagt ctcgcggcaa
    13501 aggccgcggc gtgttcgcga ccgagcgaat cgcgggaggt gaggtcatcg agcggtcgcc
    13561 ggtgatcccg attccgaagc ggcactggaa gcacatcgag aagacgatcc tgggcgacta
    13621 tgcgttcgac tggggccggg acctcgaaga cgtggcgatc gtgctcggct acgggatgat
    13681 ctacaaccac tcgcccaagc ccaacgcccg attcgacttc aagcttcgcg agcaggtcta
    13741 cgagttcatc gccctgcgcg acatcgagcc cggcgaagag atcttcacga actacaacgg
    13801 cgaccccaag gacctcacac cgctctggtt cgagaagcgc aaggcgtagg ttgccccgat
    13861 ttccccgccc actcccccat aagagggagc ggtcttccct cgcaagagcg ggcatttccc
    13921 ccttgtggtc aatagtccgg aataggagta tccctagaag gagtgggact ccgttccctc
    13981 tccggagccg cggacgccgc actcttcgac gccatcgagc gactgccggg gggaccggac
    14041 cggaccggat ggggcgccga ggggcgccgg agggagggac cgatgcgtct atcggaccga
    14101 ggccgcgccg cggccggacc ggccattgcg ctggtccttg tcgcttcgac cctcgtgacg
    14161 gcggcgcccg cgtcgccgtc gtcgtcgtat ctggtggtct gggccgggga ctggaacgcg
    14221 ggagatgcgc gtgcgggcgc gccggatctg gcgatcgaag cgggagccga cctggcggcc
    14281 gggccggatt tcctcgccgt cgtggacgcg gatccgtcgt cggcctccta cgggaaggtc
    14341 gttcgcacgc tgccggtctc gttgctcgaa aacgagccgc accacatgca gtacgcgtgg
    14401 agcccgggtg accggatctt cgccggcggg ctgttcagcg accggacgtt cgtcttcgac
    14461 gtggactcga tcccggacgt gcggctcgaa ggcgtcgtcg agccgctgga caccccgtgc
    14521 ggatccgtgc cggacgccta ctgggtgctc tcggacggca ctgcgtacgg cacgtacatg
    14581 ggcggcccca acgtggccgg agacccgcgc tgcaacggcg ggatctcgaa cgggttcgcc
    14641 ggcacgcccg gatcgatcgt gcgtatctcg cccgacgcga aggtcctgtc cgagacgtcg
    14701 gccgcgggag cgttcaacca gccgcatccc aacgacgccg gccggcccgc gcagtgcgtg
    14761 tccaatcctc cgctcgcgcg gccgtcgtgc gcgaacccgc acggcattca ggtccgtgaa
    14821 gacttgcacg tgatgatcac ctccgactac gcggagccgc gcaacgtccc gatcgatccg
    14881 gtctcggcgc cggacccgaa cgtgttccgc gacaccgtcc gcgtgtggga cattcgcgac
    14941 cgcaaccggc cggtgctgca ctcggtgtcg gtgatgccgc gtggctcgat ggccttcgat
    15001 cctccgccgc aggtcgacgc gcgcggcgtg atggggatca tggagaccac ggtcacgaac
    15061 gtgccgcagc accgcggcgc gttcgcgcag agcatgtgcg gcgggcagat ctactactcg
    15121 gccgatatca cggtgaagaa cccgatgtgg cggcaggtct tcgacgtgag cgtcgcggcg
    15181 aacgcgctcg atccggacgt cggccgcgta cagggatgca ccggcggcgg ctggttgcag
    15241 accagccccg acgacacgac cctgttccac acggtgatcg gccgcaacgc gagaaccttg
    15301 gggccgtccg atccgggtgt gcccaagatg ctcatcgcga tcgacatccg ctcgttggtc
    15361 gcgatcccgc cgtcgcagtg gacgctcgac tcgaccacca tctgcacgat cgacacggcg
    15421 gccgaggtcg tcgccggcgg ctcggagccg tcgtgcccgt cggtggcggg tgtggtcgag
    15481 ctcgccgacg cgacgaccgg cggaccgcac tggggtgcga tcgacaactt ctctctgtcg
    15541 agcgcacccg gccgcctgac ctcggtgacg cgcatcgccg tgtcgaacta cttcgtcgga
    15601 cggtcgggtc tcgacggcaa ccacggcttg tgcatggtgg acgtcgccgc cggcggatcg
    15661 ctctcgatcg acggcggctt ccgtgacgag cacaccggtg acgcgtgcgt gtcgttcgac
    15721 cggccgatct ggccgcacgg aacctatggc cgggccaaac cgcattcaat gcttttcgtc
    15781 acaccgccgt agcgccggag ctgaggaggt gagcacgcac cgcagtcgaa ccgttaccag
    15841 atcaaccggg gggaatcagg catgagtgca ctcgttcgca gcctgcgccg cgcacccgcg
    15901 atcgcgatcg tcgtcggatt gatgatgtcg atcaccgcga ccgggcccgc gtcggcgtca
    15961 cgattcggga aggccgggcc accgcagttc ccgtccttcc cctacgaaga gcgtctggga
    16021 tccgggggtc cgcccccgtt catccaggac gccgacgatc cgcacaagtg ggtgtggacg
    16081 atcgacccga gcaagtacgc cgcaaccggg acgatcacgt tctgggacta ctcgaacgcc
    16141 atcacccgcg aggggttcat gagcggcccc ggcggagcga gcgtcaacga ctttcaggtg
    16201 acctcacccg tcacgggcac gaagggattc gatttcgagc ggccgtcgtt cacgcaggac
    16261 gtcgactccg tcgaggcgga cttcctgact ccggatccgc ctcacgacat ccacacggac
    16321 ttcgacccga cggcctacca tcccgacgag ttcccgatgc ccacgccgtt cgcgacgtgg
    16381 acgggtgcaa acatcgacgg cgtcgccaac ctctacaagt tcgcgtactc ggtggcgccg
    16441 ggaacacagt tcatgaacat gcaggtcgat aaggcgggca actattacgt cgcgcgccgc
    16501 gacatgcagt gggagtggat cgacgagctc gtgttcaaga attccgacgg tgacatccag
    16561 cgggaggaca cgcagttcaa catccagccc tatccgatca gcgatgcgta cggctggtgc
    16621 ggagcgctcg tgatgcgcga cccgagcagc ctgtcgtcga tggcggggca gctaatcaag
    16681 gacatcgcct tcgacgtgtt cgcggccgac acctccccgg cgtccgccat cgctcccgtc
    16741 gcgcgagagg tcgttccctc cttcgtgatg cgctcgttcg gcatctacga ggtggacgtc
    16801 acgatgctct cgacgggcgt gcgccagcag ttcaccggca tcgccaaggg cgtgcacatc
    16861 cgtccggtcg ccgtcaccat cggcagcgtc acctatccgg ccggatcgcg cgtcgagccc
    16921 ggcgacccgg atttctcgca ctggctcaat cgcgtctcgc cgttcgcggc cggcgtcgtg
    16981 ccgatcggcg catgggtcat caacgagggc actccgtaca tgcaggtcgt tcccgcggga
    17041 acgcccggtg cggtctggca ccagaacaag ttcgccggcg cggcgttcct ggtgcgcggc
    17101 gacatggagc ggcgggtcaa ctacatcaac accgcctccg gcttggccgc cgatcaggcg
    17161 accgtcaccg gctataacga ggtgtaccac agcctgattc agctcgggct gactaactcg
    17221 aagaccggcc agtcgtggaa gcgcgcggat tgggcggact gggaggtcgg cggcacggtg
    17281 cccgtcgcca acgggtactg agccgaagat cgtatcggcg gcggagcggg atgaccgctc
    17341 cgccgccgac gatcagcact cgtccgatcc cgagaccggc ggcgccgcat cggtcgcgac
    17401 ggacacgaag aaggtgtaga cgcggccttg gatgcgggat gcggcctccg gggcgtagcc
    17461 cacgaccgcg gtcgcgaccg ttcgtcctcg gctcgcgacg accgtgtaga gcggctcggg
    17521 accgccctcg ctgcggacgc acgaatgaag cttccatccc ttgtcgggaa gctgggtgcg
    17581 gagcatcgtg gtgagcgctt cccctcgaag ctcgctcgtg aagtagacga cggcaccgat
    17641 cggcgagacg gcgctgtaca tcacggtccg tttcggcggc aacgggaagc cggccgggaa
    17701 ctcgcgggga agagcgttgc tggtggagac gcgctcgttc gggacctcgc cggggctcgc
    17761 gaccggccgc ggctcgtcac cgccgcacgc gatcagcgcc acgccgatcg cgtgcgcggc
    17821 gatcacccgg cgaaaccccc gactcatggc tcgatggtag ccggatcact tcccggtgcg
    17881 aaacgtgggc ccccagaggc tcgaactctg tccgccggat taaaagtccg gtgctctacc
    17941 cgttgagcta ggggccctcg cgcggaactc tagcaacacc ggacggcaat cgagccggac
    18001 cgcctgcgat ggtccggctc gtcgcgcaca tccctgcgga gtgcgccccc gttggaaagg
    18061 cggtgcggag acggggcatc cgcagcgcct tgaagtctcg ttccccggga gacggccggt
    18121 gaggatcggc gcggaacctc gggacggggt ccgattcgcg cacccctcgc tgtctcgtgt
    18181 ccgtccctcg ggcctgcgcc ggcgggtctc cgcaccggcc gcttgttccc ggtgcccgca
    18241 tcctgcccgg cgccggccgc ggcgttggca gcgctcgcga cacagcgagt gtgtccggcg
    18301 acacacagca agaagacccg ggcaaggggg gacggtctgc ccgggtcttc tcgtggtgcg
    18361 agtcaggcgt agaagatggt ggtcacccag taagcacccg tcgggccgcg gcagatgccg
    18421 acgccgagcg cccgatagcg tcgctcaagg atgttgtccc ggtgcgaggg cgacgccatc
    18481 catgccttgt ggatctcgta caggtcccat ccgacggcga cgttctcgcc gatgatgcgc
    18541 cagccgcgga cctgccgcgt caggttcggg ttgtggtgca gccggtcgct tcgcgacatc
    18601 gcgcacgagt ggtttcgtgc ggtgttcgac agcgactccc agaccgcgac cgagcggatc
    18661 cgtcgcttgt agtgctcgtg gttgatg
//
LOCUS       DATLFO010000175         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_47102, whole genome shotgun sequence.
ACCESSION   DATLFO010000175 DATLFO010000000
VERSION     DATLFO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47102"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="VM841_07050"
     CDS             complement(<1..1962)
                     /locus_tag="VM841_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVL89973.1"
                     /translation="MLAAMLPARASAAQITIVRDRFGVPHVYGATAADVSYGAGYALA
                     QDRLWQMHVFRMVAKGRLSHLLGSLYASNDKVIRFWTYTAAERAERFATYPADIRENL
                     TAFVAGANAYIAEVRADPARKLPLEFAQFGEPLDDWTLDDSLAMADYLIYTFGSGGGS
                     EVNNLSILQQLKAKFGDEAGAAAFDDIVWTNDPDAPASIPEGFDWHANDSRARPEADA
                     KTLHEDARLGMIEIDKVAGSAAGVSGLTQDAIARIPVSPHVLRDIEALTALDDPFSEL
                     FIKFGSNAQIAGPDVTDTGNTMGTAGPQVGLFVPQALTDFGLHSADGKLDATGMTFAG
                     AGPAVLIGRGRGYHWTTTTGASDITDTFVEKLHPENQRLYLFNPDPSDPSQARWENMK
                     CRTEVYAIKGVQFDTQEICRTRHGPVLSWDVANGVAYAARYAWFNREGGTIEGFFRYN
                     EARSIEDFATFSNLLASNHNMFYTDDQGSIGYWHPGNFPVRAPGDLRLPFSGTGEQEW
                     RGLLKAQQTPHAVYVHGDGDPNDFSRQWLANWNNKPAVDWDRENGWGAFDRTKSLIEA
                     LDPATPVADPWGGTINGDGKISWQDLNANLRYGAYRDFNADFIQRFLPAAGANANEQR
                     ALDACKGYNGAIYDDNGDGFVDSNCYTILN"
BASE COUNT          322 a          688 c          782 g          381 t
ORIGIN      
        1 gttcaggatc gtgtagcagt ttgaatccac gaagccgtcg ccgttgtcgt cgtagatcgc
       61 gccgttgtaa cccttgcagg cgtcgagcgc gcgttgctcg ttcgcgttgg cgcctgcggc
      121 cggaaggaac cgctggatga agtcggcgtt gaagtcgcgg taggccccgt aacggaggtt
      181 cgcgttgagg tcctgccacg agatcttccc gtcgccgttg atcgtgccgc cccaggggtc
      241 ggcgacgggc gtcgccggat ccagagcctc gatcagcgac ttcgtgcggt cgaaggcgcc
      301 ccatccgttc tcgcggtccc agtcgacggc cggcttgttg ttccagttgg ccagccactg
      361 ccgcgagaag tcgttgggat cgccgtcgcc gtgcacgtac accgcgtgcg gcgtctgctg
      421 tgccttgagg agtccgcgcc actcttgctc gccggtgccg ctgaagggta gtcgcaggtc
      481 gccgggcgcg cgcacgggga agttccccgg gtgccagtag ccgatgctgc cctggtcgtc
      541 ggtgtagaac atgttgtggt tggacgcgag caggttcgag aaagtggcga agtcctcgat
      601 cgaccgggcc tcgttgtatc ggaagaagcc ttcgatcgtg ccgccttcgc ggttgaacca
      661 cgcgtagcgc gcggcgtacg cgacgccgtt ggcgacgtcc caggacagca ccggcccgtg
      721 tcgcgtgcgg cagatctctt gcgtgtcgaa ctgcacgccc ttgatcgcgt acacctcggt
      781 ccggcacttc atgttctccc atcgcgcctg ggacgggtcg gacgggtcgg ggttgaacag
      841 gtagaggcgc tgattctccg ggtggagctt ctcgacgaag gtgtcggtga tgtcgctcgc
      901 gccggtggtc gtggtccagt gatagccgcg tccccgcccg atcagcacgg cggggccggc
      961 gccggcgaag gtcatgccgg tcgcgtcgag cttgccgtcg gcggagtgca ggccgaagtc
     1021 ggtcagcgcc tgtggcacga acaggccgac ctgcggtcct gcggtgccca tcgtgttgcc
     1081 ggtgtcggtg acgtcgggcc cggcgatctg cgcgttcgat ccgaacttga tgaacagctc
     1141 ggagaacgga tcgtccagcg ccgtcagcgc ctcgatgtcg cgcagcacgt gcgggctgac
     1201 cgggatgcgc gcgatcgcgt cctgggtgag gccggaaact cccgccgcgg aaccggccac
     1261 cttgtcgatc tcgatcatgc cgaggcgagc gtcctcgtgc agcgtcttgg cgtctgcctc
     1321 agggcgcgcg cggctgtcgt tggcatgcca gtcgaaacct tcggggatcg acgcgggcgc
     1381 gtcggggtcg ttcgtccaga cgatgtcgtc gaaagcggca gcgccggcct cgtcgccgaa
     1441 cttcgccttg agctgctgca ggatggacag gttgttcacc tcggagccgc cgcccgagcc
     1501 gaacgtgtag atgaggtagt cggccatcgc cagcgagtca tcgagggtcc agtcgtccag
     1561 cggctcgccg aactgggcga actccagggg aagcttgcgc gcgggatctg cgcgcacctc
     1621 ggcgatgtag gcgttcgcgc ccgcgacgaa cgcagtgagg ttctcgcgga tgtcggcggg
     1681 ataggtcgcg aagcgctcgg cacgctcggc tgcagtatag gtccagaagc ggatgacctt
     1741 gtcgttggac gcgtacagcg agcccagcag atgggacagg cgccccttcg ccaccatacg
     1801 gaagacgtgc atctgccaca agcgatcctg ggccagcgca tagccggcgc cgtacgacac
     1861 gtccgcggcg gtggcaccgt agacgtgcgg cacgccgaag cggtcgcgca cgatcgtgat
     1921 ctgtgcggcc gaggcgcgcg cggggagcat cgcggcgagc atcgcggcac acgcaagtag
     1981 ggcagggacg gctcgggaac gaagacgcgc aggcatccgg acctcctcga gggaactcga
     2041 ggtaccgatt cgacgccttc gcgcccttcc ctgcctagaa gcggcggcga gggcgcttgc
     2101 cgagcaggcg gctcggtcca cggccgccct ggtacacgac ccgcggcggg tggaccttca
     2161 tgccggcttc gtg
//
LOCUS       DATLFO010000176         9638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_13325, whole genome shotgun sequence.
ACCESSION   DATLFO010000176 DATLFO010000000
VERSION     DATLFO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13325"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1340
                     /locus_tag="VM841_07055"
     CDS             84..1340
                     /locus_tag="VM841_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVL89974.1"
                     /translation="MIYLWGLVGVLVVAGSLLAMAETSISRLNRVRAMALREEGRKNA
                     DMLERIESDPARYLNSVYLAVMFAQNGSAILVATLVERRYSSVWVTVASAAFTLLYFV
                     IVEAMAKTYGILHSDRVALALSPFVALLARLLAAPTRALIGLANVLLPGKGLKQGPFV
                     SEEDIRSMADVGHEEGAIEEEEKELIHSIFEFGDTVVREVMVPRPDMVTIEVTMTPRQ
                     ALSLMLKNGFSRVPAHEGTDDNIVGLVYAKDIMRRLSGRKRDVKLAEILRPAIFVPDS
                     KRISELLREMQKSKTHMAIVSDEYGDVAGLVTIEDLLEEIVGEIADEYDREEPSVQPV
                     DESTIRVNGHLTIEDLNEVLDTELPNQEWDTVGGLMAGILGKIPSLGDEVTVDGITFR
                     ADRVQGRRIAKVLIHKPDGLPEAAGE"
     gene            1340..2233
                     /gene="era"
                     /locus_tag="VM841_07060"
     CDS             1340..2233
                     /gene="era"
                     /locus_tag="VM841_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVL89975.1"
                     /translation="MLASEGYRSGFISIVGRPNVGKSTLLNAFMGRKVAITSPKPQTT
                     RNAIRGVLTGEDWQMIFIDTPGLHKPKTLLGERLNDVVRGTLKEVDAIVFMLDATQPV
                     GTGDQFVAQAVLQAGTPAICVVNKMDIAGPDKLAPQLQSADALGDWHAIIPISAAQGT
                     KVDELQELLVGLLEEGPQFYPDDVVTDQPRDLLLGEIIREKAITLTHQEVPHSIAVAV
                     QEVVEREDGVIEVYATVFVERDSQKGIIIGKGGSMLKEIGTRARQDLEWVLGGHIFLK
                     LQVKVAKDWTRDPKHLQRLGY"
     gene            2373..3089
                     /gene="recO"
                     /locus_tag="VM841_07065"
     CDS             2373..3089
                     /gene="recO"
                     /locus_tag="VM841_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HVL89976.1"
                     /translation="MALYKEQGIVLRTMKLGEADRIVTVMTQGSGKIRAVAKGVRKTK
                     SRWGARLEPFTHVDLVLYRGRDLDIVTQAQILSPFTPIRSDYARFAAGEVIMEAVDKI
                     TQDREKSVRTFMLLLGALRALSLPDEDPLMVADSFLLRLAALAGFRPALTACAVCNRP
                     GPHGKFSVAQGGSVCDSCRAGAAVTVGEDAIPYLDSLLNADWSAETPEVVRRECSGLI
                     RAYVEYHFDRPIRSWTHVPR"
     gene            3112..3834
                     /gene="uppS"
                     /locus_tag="VM841_07070"
     CDS             3112..3834
                     /gene="uppS"
                     /locus_tag="VM841_07070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HVL89977.1"
                     /translation="MKTDGIDPRALPRHVAIVMDGNGRWAKQRGLPRTEGHMEGQRTV
                     VKIVDAALELGIPWLTLYAFSTENWRRPPGEVRYLLGDTERFVRDNRDGFHAKDVRMR
                     WVGRPNRVPKRILRQIEESIEMTRRNKAMTLTIAFNYGSRAEITDAVKAIAGQAASGA
                     LDADKITDKTVARAMYDPEMPDVDLFIRTSGEQRISNYLLWQSAYAELLFSPVLWPDF
                     SRDDLIEAVRDYQGRGRRFGGV"
     gene            3834..4376
                     /locus_tag="VM841_07075"
     CDS             3834..4376
                     /locus_tag="VM841_07075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVL89978.1"
                     /translation="MRIDGDAVFLRPLALDDAEEFRDLRVRNREFFRPYEPLMADSDF
                     TPAAIRRQLEISLDEDRSGMSYSFGIFDRAGGAMAGRIRLSNVFRGVWKNANVGYYVD
                     QDHGGRGFATEAVRLVCRFAFSDADLHRVQAGVMPENERSIRVLEKAGMRREGLALRY
                     LLINGAWRDHYLYAITAEEL"
     gene            complement(4427..5197)
                     /locus_tag="VM841_07080"
     CDS             complement(4427..5197)
                     /locus_tag="VM841_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HVL89979.1"
                     /translation="MSLRPSELLYRYYEARLARELRGGPMPGHVGIILDGNRRWAAAM
                     GYADAAEGHRRGAEKIAELLDWCEELRIGVVTIWLLSTENLRRSPEELRALLEIIEGT
                     VDSLATAGRWRIRTLGSLEMLPDSTVDVLREAEARDPGGDGGILNVAVGYGGRQEIAD
                     AIAQMMESYAADGLSLDEAAKKVSPDEISRFLYTAGLPDPDLVIRTSGEVRMSGFLLW
                     QSAHSEYYFCDPYWPAFRRIDFLRAVRAYQQRHRRFGR"
     gene            5391..7181
                     /locus_tag="VM841_07085"
     CDS             5391..7181
                     /locus_tag="VM841_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL89980.1"
                     /translation="MSEQPGTDRTRDGRAAFLAEICRVALDARDASTLFRDACALMSG
                     VFGAGAVVTVESAPVRAEGTTSVVIPRAAGAVVVVRCLEPFTRADIEFVETIAGLLAP
                     ALERIRFEEALRASEQRMLLAQHIAHIGSYDWDIATDTNAWSDELYRIYGTEPQSFNA
                     SYEKFLSFIHPDDRERIVALHKRAYETGESYQMEERIIRPDGEVRVLSSNGKVIRDAS
                     GIPVRMVGVCMDVTEQRLAEETSKRESERFRALIESAPDAVLVIDPDGAIVQTNQQAE
                     VLFGYSRDELSELRVDDLLAPESRARHAEHRASYLSDPKPRPMGTALDISARRKDGTD
                     VYLDVGLGVLRTGDGILVAAFARDATPRREAEIAKRRLGEIEQRRRQALEINDNVVQG
                     LVAALTSIEAGRFDLGAEAVERTLRSARGMMNDLLGDERAMQAGELVRERPAESHMDH
                     GDATPSLRVTPDVADRPPVRSVVIADDSDDLRFLLKMLLSRSEDFEVVDEACDGQAAI
                     ESVRAHRPDLILLDLAMPRMDGLEAVPAIRKVSPETVVVVVSGFDSARMSGAVSDAGA
                     DAYIEKDASLTDIAAMLMQICDRGPVARAI"
     gene            complement(7178..7564)
                     /locus_tag="VM841_07090"
     CDS             complement(7178..7564)
                     /locus_tag="VM841_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HVL89981.1"
                     /translation="MDIEAALRQIEANGLGTLGTIGTAGPPHLSVVFAAVVEGELWIS
                     STQDRRKTKNVRANPHASFLSGTGPWAAIEGTARVDDGEDIAERLRRYYRTASGEHPD
                     WDEYDAAMVAEKRLVIAITPVRAYGA"
     gene            7619..9004
                     /locus_tag="VM841_07095"
     CDS             7619..9004
                     /locus_tag="VM841_07095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HVL89982.1"
                     /translation="MPPTMDSIVNLCKRRGFIFPSSEIYGGLRSTWDYGPIGVELKRN
                     VKNAWWRWMVQLRDDVVGLDAAILMHPRTWEASGHVETFSDPLVECLSCHQRFRADHL
                     PGFQDASGHEDVEGETVDMGKVGAACPNCGNREFTDPRNFNLMFKTYMGPVEDATAVV
                     WLRPETAQGIFVNFAQVQTTTRKKLPFGIAQVGKSFRNEITPGNFVYRTREFEQMEME
                     FFCKPGTDEEWHQYWIDERLGWYKRYGIKDDNLMLREHAQDELSHYSKRTVDIEYRFP
                     FTDWGELEGIANRTDFDLKRHGEFSGQDLTFFDQEANEHYVPYVIEPAAGADRATLAF
                     LIDAYTEEETTSASGKTETRTVLKLHHELAPMKAAVLPLSRNEKLVPTAKEVAAQLRP
                     HFTIDYDDAGSIGRRYRRQDEIGTPFCVTVDFDSIDDKAVTIRDRDSMTQERIAIADL
                     AGYLGDKLKPV"
     gene            complement(9012..9407)
                     /locus_tag="VM841_07100"
     CDS             complement(9012..9407)
                     /locus_tag="VM841_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL89983.1"
                     /translation="MAQFPDIGHIALTVSDASASRVWYSKLFGAEPVTDEDTGPFRHV
                     VYKLGDTLFGLHQHTQPPAPNDSFNEFRIGLDHVSFHVGSRGELEKWRAKLDELGIAN
                     NAINDTPDGSALAFRDPDNNALELFAFPG"
BASE COUNT         1624 a         3226 c         3237 g         1551 t
ORIGIN      
        1 cttgcgggac gtgatcacgc caccccggcc gacgagatcg cgatggcagg cgaggagcgg
       61 agcatcgtgg ccgcgatgat cgcatgatct atctctgggg actcgtcggc gtcctcgtcg
      121 ttgcgggctc gctgctcgcg atggccgaga cgtcgatctc gcgcctgaac cgcgtgcgcg
      181 cgatggcgct tcgcgaggaa ggccgcaaga acgccgacat gctcgagcga atcgagtccg
      241 atccggcgcg gtatctgaac tcggtgtacc tcgccgtgat gttcgcgcag aacggctcgg
      301 cgatcttggt cgcgacgctc gtcgagcgtc gctactcgag cgtgtgggtc acggtcgcct
      361 cggccgcgtt cacgctgctg tacttcgtga tcgtcgaggc catggccaag acctacggga
      421 ttctgcattc ggatcgcgtc gcgctcgctt tgtcgccgtt cgtggcgctg ctcgcgcgac
      481 tgctcgcggc gccgacccgg gccctgatcg ggctggccaa cgtgctcctg cccggcaagg
      541 gcctgaagca gggtccgttc gtgtccgagg aggacatccg ctcgatggcc gacgtcggcc
      601 acgaggaagg tgcgatcgag gaggaagaga aagagctcat ccactcgatc ttcgagttcg
      661 gcgacaccgt cgtgcgcgag gtgatggtgc cgcgtcccga catggtcacc atcgaggtca
      721 ccatgacgcc gcgacaggcg ctgtcgctga tgctcaagaa cggattctcc cgggtgccgg
      781 cgcatgaggg caccgacgac aacatcgtcg ggctcgtgta tgcgaaggac attatgcggc
      841 gcctgtccgg ccgaaaaaga gacgtgaagc tggccgagat cctgcggccg gcgatcttcg
      901 ttcccgattc gaagcgaatc agcgagctgc tgcgcgagat gcaaaagtcc aagacccaca
      961 tggcgatcgt ctccgacgag tacggcgacg tcgccggcct ggtcacgatc gaagacctgc
     1021 tcgaggagat cgtcggcgaa atcgccgacg agtatgaccg cgaggagccg tcggtccagc
     1081 ccgtggacga atccacgatc cgcgtcaacg gacatctcac gatcgaggat ctcaacgagg
     1141 tgctcgacac cgagcttccc aaccaagagt gggatacggt ggggggcctg atggcaggaa
     1201 tcttgggcaa gatcccgtcg ctcggtgacg aggtgacggt ggacggcatc accttccgcg
     1261 cggaccgcgt gcagggccgc cgcatcgcca aggtcctgat ccacaagccg gacgggctgc
     1321 cggaagcagc gggcgaatag tgctcgcgtc cgaaggctac cggtcgggct tcatctcgat
     1381 cgtcggacgg cccaacgtcg gcaagtccac cttgttgaac gcgttcatgg gccgcaaggt
     1441 cgcgatcacc tcgccgaagc cgcagacgac gcgcaacgcg atccgcggcg tgctcaccgg
     1501 cgaggactgg cagatgatct tcatcgacac ccccggcctg cacaagccca agaccctgct
     1561 gggcgagcgg ctcaacgacg tggtccgcgg cacgctcaag gaagtcgatg cgatcgtgtt
     1621 catgctcgat gcgacgcagc cggtgggcac gggcgaccag ttcgtcgccc aggcagtgct
     1681 gcaggccggt acgcccgcga tctgcgtcgt caacaagatg gacatcgccg gcccggacaa
     1741 gctcgcgccg cagctgcagt ccgccgacgc gctcggcgac tggcacgcga tcatcccgat
     1801 cagcgccgcg cagggcacga aggtcgacga gctgcaggag cttctcgtcg gcctgctcga
     1861 ggaggggccg cagttctatc ccgacgacgt cgtcaccgac cagccgcggg acctgctgct
     1921 gggcgagatc atccgcgaga aggcgatcac gctcacgcac caagaggtgc cgcactcgat
     1981 cgccgtcgcc gtgcaagaag tcgtcgagcg cgaggacggc gtgatcgagg tctacgcgac
     2041 cgttttcgtc gagcgcgaca gccagaaggg catcatcatc ggcaagggcg gctcgatgct
     2101 caaagaaatc ggcacgcgcg cccgccagga cctggagtgg gttctcggcg ggcacatctt
     2161 cctgaagctt caggtcaagg tcgccaagga ctggacccgc gaccccaagc atctgcagcg
     2221 gctcggctac tgacgcacca ggtggacgat gaaaccggtg gccgaacggc cccgtcagct
     2281 tcctgcgatg cactcgtcaa ttttcttgac gagtgcatcg gatacgcatc aggcctgcgg
     2341 tgggcgtccg cccgccgtag gatgaggcgg tcatggcgct ctacaaggag cagggcatcg
     2401 tcctgcgcac gatgaagctc ggcgaggccg accggatcgt cacggtgatg acgcaaggct
     2461 cgggcaagat ccgcgccgtc gcgaaaggcg ttcgcaagac caagtcgcga tggggcgccc
     2521 ggctcgagcc gttcacgcac gtcgacctgg tgctctaccg cggacgcgac ttggacatcg
     2581 tgacgcaggc gcaaatcctg tcgccgttca ccccgatccg cagcgactat gcccgcttcg
     2641 ccgcgggcga ggtcatcatg gaagcggtgg acaagatcac ccaggatcgc gagaagtccg
     2701 tccgcacgtt catgcttctg ctcggcgcgc tgcgggcgtt gtcgctgccg gacgaggacc
     2761 cgctgatggt cgcagactcg ttcttgctgc gcctggccgc gctggccggt ttccggccgg
     2821 cgctgaccgc ctgcgcggtg tgcaaccgtc ccgggccgca cggcaagttc tcggtcgccc
     2881 agggcggctc ggtgtgcgac tcctgccggg cgggagcggc ggtcacggtc ggcgaggacg
     2941 cgatcccgta cctggattcg ctgctcaacg cggactggtc cgccgaaaca cccgaggtcg
     3001 tgcggcgcga atgctcgggc ctgatccggg cctacgtcga gtatcacttc gaccgcccga
     3061 tccggtcgtg gacgcacgtg ccccggtaga tcgcgagtag cattcgcgtc cgtgaagacg
     3121 gacggcatcg atcctcgcgc gctgccgcgc cacgtcgcca tcgtcatgga cggcaacggc
     3181 cgctgggcca agcagcgggg cttgccgcgc accgaggggc acatggaagg gcagcgcacg
     3241 gtggtcaaga tcgtggacgc cgcgctggag ctcggcatcc cctggctcac cttgtatgcg
     3301 ttctcgaccg aaaactggcg ccggccaccc ggcgaggtgc gttacctgct cggcgacacc
     3361 gagcgcttcg tgcgcgacaa ccgcgacggc tttcatgcga aggacgtgcg catgcgctgg
     3421 gtcgggcggc cgaaccgggt gcccaaacgc atcctgcgtc agatcgaaga gtcgatcgag
     3481 atgacgcgtc gcaacaaggc gatgacgctg acgatcgcgt tcaactacgg ctcgcgcgcc
     3541 gagatcacgg acgccgtcaa ggcgatcgcc ggacaggccg cgtcgggagc gctggacgcg
     3601 gacaagatca ccgacaagac ggttgcgcgg gcgatgtacg acccggagat gcccgatgtg
     3661 gacctgttca tccgaacctc gggcgagcag cgcatctcga actacctgct ctggcaatcg
     3721 gcctatgccg agctgctttt ctcaccggtg ctgtggccgg acttctcgcg tgacgatctg
     3781 atcgaggcgg tgcgtgacta ccagggacgc ggccgccgtt tcggaggcgt ctgatgcgca
     3841 tcgacggcga cgccgtgttc ctgcggcccc tcgcgctcga cgacgccgag gagtttcgcg
     3901 acctgcgcgt gcgcaaccgc gagttcttcc gcccctacga gccgctgatg gccgactcgg
     3961 acttcacgcc cgctgcgatc cgtcgccagc tcgagatctc gctcgacgag gaccgttcgg
     4021 ggatgtcgta ctcgttcggc atcttcgatc gcgccggcgg cgcgatggcg ggccggatcc
     4081 ggctcagcaa cgtcttccgc ggcgtgtgga agaacgcgaa cgtcggctat tacgtcgatc
     4141 aggatcacgg gggacgcgga ttcgccaccg aagcggtccg gctggtgtgc cggttcgcgt
     4201 tctccgacgc cgacctgcat cgggtccagg ccggcgtgat gcccgagaac gagcgctcca
     4261 tccgcgtgct cgagaaggcg ggcatgcggc gggaggggct cgcgcttcgc tacttgctga
     4321 tcaacggcgc gtggcgcgac cactacctgt acgccatcac cgccgaagag ctttagccgg
     4381 ccttctgctc ccgggcctcc cgtccggctt catctgtcgc gcgcggctac cggccgaaac
     4441 ggcggtgacg ctgctgatac gcgcgtacgg cgcgcaagaa gtcgatgcgc cggaaggccg
     4501 gccagtacgg atcgcagaag tagtactcgg agtgcgcgct ctgccagagc aggaagccgg
     4561 acatgcggac ctcgcccgag gtgcggatca ccagatccgg gtcgggcagg ccggccgtgt
     4621 acaggaagcg cgagatctcg tcggggctga ccttcttcgc ggcctcgtcg agcgacagtc
     4681 cgtcggccgc gtaggactcc atcatctgtg cgatcgcgtc cgcgatctcc tgacggccac
     4741 cgtagcccac agcgacgttg aggatcccgc cgtcaccccc cggatcgcga gcttccgctt
     4801 cccgcagcac gtccacggtc gagtcgggca gcatctcgag gctgccgagc gtgcggatgc
     4861 gccaccgtcc cgccgtggcc agcgagtcga ccgtcccttc gatgatctcg agcagggcgc
     4921 gcagctcctc ggggctacgg cgcaggttct cggtcgaaag cagccaaatc gtgacgacgc
     4981 cgatgcgcag ctcctcgcac cagtccagca gctcggcgat cttctcggcg ccgcggcgat
     5041 gtccttccgc tgcgtccgca tagcccatcg cggccgccca gcgccggttt ccgtcgagga
     5101 tgattccgac atgcccgggc attggacccc cgcgcagctc gcgcgcaaga cgggcctcgt
     5161 agtagcggta gagcagctcg ctcggtcgca gggacatcgc cccgcgagcg tagcacctcc
     5221 ggcctgtccg gcccgctccg gaccatgcgc ccggcccatc ggcgagtggt caacatgatc
     5281 cgtccgggcc atataccggt cccgaggaga gccgaataat ggggcgaccg gtttcgtcgc
     5341 gcgcccgccc cctggcgaaa cgttcttgct ttcgacgagg aaccgaccac atgagcgaac
     5401 agcccgggac cgaccgaacg cgcgatggcc gcgccgcgtt tcttgccgag atctgccgcg
     5461 tcgcgctgga cgcgcgcgac gcatccaccc tcttccgtga cgcatgcgct ttgatgtccg
     5521 gcgtgttcgg cgcgggggcc gtggtcacgg tggagagcgc tcccgtccgc gctgaaggaa
     5581 ccacgtcggt cgttatcccg cgcgccgcgg gggccgtcgt cgtcgtgcga tgcctcgagc
     5641 cattcacgcg tgccgacatc gaattcgtcg agacgatcgc cggccttctg gctccggcgc
     5701 tggagaggat ccgcttcgag gaggcgcttc gtgccagcga gcagcgcatg ctgctcgccc
     5761 agcacatcgc gcacatcggc tcctacgatt gggacatcgc aaccgacacc aacgcgtgga
     5821 gcgacgaact gtaccgtatc tacgggacgg agccgcaatc gttcaacgcg agctacgaga
     5881 agttcctttc gttcatccac ccggacgatc gagagcgcat cgtcgccttg cacaagcggg
     5941 cgtacgagac cggcgagtcg tatcagatgg aggaacgcat catccggccc gacggcgagg
     6001 tgcgcgtcct ttcgtcgaac ggcaaggtga tccgcgacgc ctccggcatc cccgtccgga
     6061 tggtcggcgt ctgcatggat gtgaccgagc agcggctcgc ggaggagacc tccaagcgag
     6121 agagcgagcg gttccgcgcg ctgatcgaat ccgcgcccga cgcggtgctc gtcatcgatc
     6181 ccgacggggc catcgtgcag acgaaccaac aagccgaggt gctcttcggg tattcgcgcg
     6241 acgagctttc cgagcttcgt gtcgacgatc tgctcgcgcc ggagtctcgg gcacggcacg
     6301 ccgagcaccg ggcgagctac ttgagcgatc cgaagccgcg cccgatggga accgccctcg
     6361 atatctcggc gcggcggaag gacggaaccg atgtctacct cgacgtcggg ctcggcgtgc
     6421 tgcggaccgg cgacggaatc ttggtcgcgg ccttcgctcg tgacgcgacg ccgcggcgtg
     6481 aggccgagat cgccaagcgc cggctcggcg agatcgagca gcgccgccgg caagcgctcg
     6541 agatcaacga caacgtggtc caaggcctgg tggccgcgct cacctcgatc gaggcaggac
     6601 ggttcgacct gggcgccgaa gccgtcgagc gaacgcttcg ctcggcgcgg gggatgatga
     6661 acgacctgct cggcgacgag cgggcgatgc aagccggaga gctggtccgg gaacggccgg
     6721 ccgaatccca catggatcac ggcgatgcca caccatcctt gcgcgtgacg ccggacgtcg
     6781 ccgaccggcc tcctgtgcgc agcgtggtga tcgcagacga ctcggacgat ctgcggttcc
     6841 ttctcaagat gctgttgtcg cggtcggagg acttcgaggt cgtggacgag gcctgtgacg
     6901 gccaggcggc gatcgagtcg gtccgggcac accggccgga tctgatcctg ctcgacctgg
     6961 ccatgccgcg catggacggt ctggaggccg tccccgcgat ccgcaaggtc tctcccgaga
     7021 cggtcgtggt cgtggtgtcg ggcttcgact cggctcgcat gagcggggcc gtgtccgacg
     7081 cgggcgccga cgcctacatc gagaaggacg cgtcgctgac cgacatcgcc gcgatgctca
     7141 tgcagatctg cgaccgcggc ccggtcgcgc gcgcgatcta ggccccatag gcgcgaaccg
     7201 gcgtgatcgc gatgacgagc cgcttttcgg cgaccatggc ggcgtcgtac tcgtcccagt
     7261 cgggatgctc gccgctcgcg gtgcggtaat agcgacggag ccgctcggcg atgtcctcgc
     7321 cgtcgtccac gcgcgccgtt ccttcgatcg ccgcccacgg acccgtaccc gacaggaagg
     7381 acgcgtgcgg gttcgcgcgc acgttcttgg ttttgcggcg atcctgcgtc gaggagatcc
     7441 atagctcgcc ttcaacgacg gcggcgaaca ccaccgacag gtgcggcggg ccggccgttc
     7501 cgatcgtgcc cagcgtcccg agaccgtttg cttcgatttg cctgagcgcc gcttcgatgt
     7561 ccatgcgtcc tccgctcgtg tatcgcgtcc ggcgtgcctt cgctatgatt gcgcgaccat
     7621 gcctcccacg atggactcta tcgtcaacct ctgtaagcgc aggggcttca tcttcccgtc
     7681 ctccgagatc tacggcgggc ttcgctcgac gtgggactac gggccgatcg gcgtcgagct
     7741 caagcgcaac gtcaagaacg cgtggtggcg ctggatggtg cagctgcgcg acgacgtcgt
     7801 tggcctggat gcggcgatcc tgatgcaccc gcgaacctgg gaggcgtcgg gccatgtcga
     7861 gaccttcagc gacccgctcg tcgagtgcct gagctgccac caacgctttc gcgccgatca
     7921 cctgccgggg ttccaggacg cctcgggcca cgaggacgtc gagggcgaga ccgtggacat
     7981 gggcaaggtg ggcgcggcct gccccaactg cggcaatcgc gagttcaccg acccgcgcaa
     8041 cttcaacttg atgttcaaga cctacatggg gccggtcgag gacgcgacgg ccgtcgtgtg
     8101 gctgcgcccc gagaccgccc agggcatctt cgtgaacttc gcccaggtcc agacgaccac
     8161 gcgcaagaag cttccgttcg gcatcgcgca ggtcggcaag tcgttccgca acgagatcac
     8221 gcccggcaac ttcgtctacc ggacccgcga gttcgagcag atggagatgg aattcttctg
     8281 caagcccggc accgacgaag agtggcatca gtactggatc gacgagcggc tcggctggta
     8341 caagcgctac ggcatcaagg acgacaacct gatgctgcgc gagcacgccc aggacgagct
     8401 gtcgcactac tccaagcgca ccgtggacat cgagtaccgg tttccgttca ccgactgggg
     8461 cgagctcgaa ggcatcgcga accgcaccga cttcgacctc aagcggcacg gcgagttctc
     8521 cggccaggac ctgacgttct tcgaccagga agccaacgag cactacgtgc cctacgtgat
     8581 cgagccggcc gccggcgccg accgtgccac gctcgctttc ctcatcgacg cctacaccga
     8641 ggaagagacc accagcgcct ccggcaagac cgagacgcgc acggtgctca agctgcatca
     8701 cgagctcgcc ccgatgaagg ccgccgtcct gcccttgtcg cgcaacgaga agctcgtgcc
     8761 gacggcgaag gaggtcgcgg cgcagctgcg gccccacttc accatcgact acgacgacgc
     8821 cgggtcgatc gggcgccgct accgccgcca ggacgagatc ggcacgccct tctgcgtgac
     8881 ggtggacttc gactccatcg acgacaaggc cgtgacgatc cgcgaccgcg acagcatgac
     8941 gcaagagcgc atcgcgatcg cggacctggc cggctacctc ggcgacaagc tcaagcccgt
     9001 ctgatccggc ctcagccggg gaaggcgaac agctcgagcg cgttgttgtc gggatcgcgg
     9061 aacgcgagcg ccgacccgtc cggggtgtcg ttgatcgcgt tgttggcgat gccgagctcg
     9121 tccagcttcg cgcgccactt ctccagctcg ccgcgtgagc caacgtggaa cgagacgtgg
     9181 tcgaggccga tgcgaaactc gttgaacgag tcgttcggcg ccggcggctg cgtgtgctgg
     9241 tgaaggccga agagcgtgtc cccgagcttg tagacgacgt ggcggaacgg gcccgtgtcc
     9301 tcgtcggtca ccggttcggc gccgaacagc ttcgagtacc agacgcgcga tgcggaagcg
     9361 tcgctgacgg tcagggcgat gtggccgatg tcgggaaact gggccatcgc ggttccccct
     9421 tccttgacgg ttgaggcgat gctatcctgc ggcgccgggc agggtccaga gagctacgtc
     9481 acgtctcgcg cacggaagac acccgtggcg agcgcggaaa cgcctgcggc gtaggctgcg
     9541 agcaacaagc ccgcggcggc cacggaccgt cccgcgatct cgcgtgtgcc gtcgccgccg
     9601 acccatacga tcgcgttgcc gaccagcgac cacggccg
//
LOCUS       DATLFO010000177         9977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_24343, whole genome shotgun sequence.
ACCESSION   DATLFO010000177 DATLFO010000000
VERSION     DATLFO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24343"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VM841_07105"
     CDS             <1..385
                     /locus_tag="VM841_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89984.1"
                     /translation="YTIAVWRGGLPDDPPSARVLVRRFSWLTAEVTVEDVTIDELALR
                     LVPGSVTTIRFQAELPVTGAFDILLASAGMRVRQWVATGPAVAFLGESAAETTYGPGQ
                     LAGGQTGLVTEALWGIRGHGLVRAR"
     gene            489..1157
                     /locus_tag="VM841_07110"
     CDS             489..1157
                     /locus_tag="VM841_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HVL89985.1"
                     /translation="MELTLEAEIRQQAGKGVARKLRAAGKVPGVVYGAGEAATAIAVD
                     RRQLTHVLATDAGLNVLIDLKVGSDTYLTLARELQRDALRGDIKHVDFVKIDRNTAIE
                     VDIPIHVEGDSIGVREGGVVEHHIWSIKVSCKPGNVPESITADITSVGLHEHFKAGDL
                     TMPAGVELLTDPEENIITIATPQVLKVEAELEQPGMEPAAAAGEAAPAESEEAPAAEG
                     GEES"
     gene            1203..1784
                     /gene="pth"
                     /locus_tag="VM841_07115"
     CDS             1203..1784
                     /gene="pth"
                     /locus_tag="VM841_07115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HVL89986.1"
                     /translation="MAGEVWLVAGLGNPGAEYERTRHNVGFRVLDRLASELGDARFKR
                     GKAPAMVAETRDGDVRVILVKPATYMNLSGQAVGPLATYYKAPAACVVAVHDEIDLPF
                     GRLQVKIGGGSAGHNGIRSLVSSLGTNEFVRLRIGVGRPGGRKEAADHVLDTFSKAEE
                     KELPVVVAEAADAVLSILRDGPERAQNAVNTRN"
     gene            1870..2586
                     /locus_tag="VM841_07120"
     CDS             1870..2586
                     /locus_tag="VM841_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89987.1"
                     /translation="MKDKSEIAAIAVIVLGVALIAGLVVNENRKGETDVLGTRFASPT
                     PTAVATGDPFVIPTIDSTIVPTPTPTPTVAPATPTPTATATTTARPATTATTIARPAT
                     VAAYLVEVRCGETVMYSDGTGVGGSGGSWRETAAAGKRTSYEFNYQREQSGRQVTSQR
                     KTCEIKTERTRAGSEPSLRVTLACDGRGTYETSPPPSPSTNVISRTWRLSELQSGFTH
                     EMRNKPGQVSEQTTCEVRVV"
     gene            2719..6099
                     /gene="mfd"
                     /locus_tag="VM841_07125"
     CDS             2719..6099
                     /gene="mfd"
                     /locus_tag="VM841_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HVL89988.1"
                     /translation="MDLSRVLSDVAVSPELAEAVDALALRQAVTVADQARTVLPAVWS
                     LDPARPPMLLIAPSSRDAERITAEINAWRSGGAVLFPAWETLPHERLSPRAETIGRRL
                     EALWRLRVPPEDGVAPKVIVAPVRAAVQRMVPGVESLSPIIVRTGEWLDVQDLVRRLA
                     GAQYERVDMVGRRGEFAVRGGLVDVFPATADHPVRLDFFGDEVTSMREFAVSSQRSIR
                     DVERIVAFSCRELIPTDEVRGRAIAALERAPHLRPELMRLADGITFEGMEALIEVLWD
                     TLPVLREILPDDTLTVVLEPKRTADRAAEMLQEVEAMREAMWAAAADGARPPADNTYA
                     ELDAAIGPEPRAALSPFRGDGPALDAKPWETRDIDALAAEVRRLLQGGSRVIVAAESH
                     GSADRIRELLAGHELFLPRYDGGELPPARGAVTVAPIDEGFVSESLGLAVVGEHDLFG
                     KRRPHREPKAKRRSIIALELEPGDLVVHAIHGIGRFTGMVTREVGTGKTDYLVLEYAA
                     GDTLYVPADTLDLVTKYVGGEPPKLHRLGGKDWDKQKARVKKAVRDMADELIKLYSQR
                     AAAHGHAFGQDTPWQRELEDTFPYIETKDQLAAIEDVKADMERPAPMDRLVCGDVGFG
                     KTEIAIRAAFKAVADGKQVAVLVPTTLLAQQHYATFSERYAPFPVRVAVLSRFVTPKE
                     QKRVIDEIAAGKIDVVIGTHRLLNKDVTFADLGLLIVDEEQRFGVAHKEAIKKLKTNV
                     DVLTMTATPIPRTLEMSMSGIRDLSVIDTPPEDRHPVLTFVGPFDEPTVAAAIRREML
                     REGQVFYVHNRVHDIERSTQTIRKLVPEARIGVAHGQMSERQLEKIMLDFWDREYDVL
                     VSTTIIESGLDIPTANTIIVDRADQLGLSQMYQLRGRVGRARERAYAYFFFPPERSLT
                     EQSHARLSALSQHTELGSGLQIALRDLEIRGAGNLLGGAQHGHITSVGFDLYVQMLAD
                     AVDEARGKLPEPKPDVKVDIAVDAHLPESWIPREGLRLEAYRRVAQAPSHEALADVRA
                     ELLDRYGELPPQAETLLTVASIKLDLGLRGVRTVSAGGGKARFEPFVLAESEQVRVRR
                     LFPGSVYKTNTYTLILPMPAAETPAAWLRRAVSDILAP"
     gene            6099..7133
                     /locus_tag="VM841_07130"
     CDS             6099..7133
                     /locus_tag="VM841_07130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVL89989.1"
                     /translation="MRIRLVLPLLALALAASACGRYLEASAAVVDGKPISMADLDAAV
                     LEALRGQDDVTQEQRLETERRVLGQIIQDRIIAGEAEIRKIKVDTAAVEERYQGIRSQ
                     FDSDEELAQALAAQGVDIATVRDRIRLRLIVEAIQKELADTVKITPAQIKEAFGDGSA
                     FQEVRARHIFFAVPDPSAEQTALKKAQDALARLRAGADFAALARQISEDPRTKASGGE
                     LGVLRPGAAQAGDLERAAFRLERGEISEPLRSQAGFHLIQVTGKGKKTLEQATPEIKE
                     ALQSQGAQEAFTEFITTRIRAARVEVNPRFGEFDPAQVTIVDRQFYTPAPVDPNGVPG
                     LQLPLDEVSG"
     gene            complement(7130..7642)
                     /locus_tag="VM841_07135"
     CDS             complement(7130..7642)
                     /locus_tag="VM841_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89990.1"
                     /translation="MTVRIRDARNVELDDVSALMVTAYGQYEAELPAHLWEDYRAEIA
                     DVRRRMPFSQLIVAEHENALCGAVTFYPDATKSEHLGWPEGFTEVRLLSVDPAARGLG
                     IGRLLTDECIRRAKDLGAHTIGLHTSYLMWVARDMYERMGFVRAPEFDFVPEPNELPD
                     LVAIAYRLEI"
     gene            complement(7639..8175)
                     /locus_tag="VM841_07140"
     CDS             complement(7639..8175)
                     /locus_tag="VM841_07140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL89991.1"
                     /translation="MVRSPIEMDVVVATDDRIDEIASLLRLAYAEFAPPPGDSDRPSW
                     DDYYDHEIPDVRGRWPFTSHLIVVEDDAVIGTACYIPDAADERMAEDRWPEGYAAMRL
                     LGVHPRARGRGIGRRLTEACLARAREQGAEWFGLHTTHLMSVARGMYERLGFERFPDN
                     DIPVSDTFVVIAYRTKLA"
     gene            8201..9202
                     /locus_tag="VM841_07145"
     CDS             8201..9202
                     /locus_tag="VM841_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVL89992.1"
                     /translation="MIGILGAGAMGAALAAHLAREGRDVVLCATEFDERAAHAVEHAL
                     PHPGLGIALGDGIRVVRSPQWPEAIASMRIITVAVSTDGLAAVIEAAASHAAPDALWA
                     IATKGWEESTLRSSATVACDTLGDAKRVVQLVGPSLAAEVAAGVPTGIVAAGVHLPAA
                     IAVAEAFASPLLHVRATTDVAGVEIGSALKNVIAIALGLCDGIAEHEGRPWTNTKAML
                     FSRGLSEMRELVRVSGGRGSTVLGLAGSGDLYVTALGGRNARFGRLVGLGTPPEKALE
                     EMNTTVEGYANAPAAAALAAKHGLDLAIVRAVNAVLFEGLPPAEAIRSLSGGVGVDE"
     gene            9252..>9977
                     /locus_tag="VM841_07150"
     CDS             9252..>9977
                     /locus_tag="VM841_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89993.1"
                     /translation="MNRGARGHENDSGETFDASDAAEVDDDAMIREPVHEDRADIEEP
                     ARDLHEDTPTAERLSRVAEGLSRVAEELGRGSEHLRAKPEPRKEPRKEPRKEPPKEPG
                     KEQAKEAVKRRSKEAAKEAAKEAAKEAAPAASSGAGAGNVAGGDSGAGDVESEDGMSA
                     PTIHPAPEAAPPAEETPTVELADMERSEEPGIVPPAAVDAPTVELTAEQDPAPSAPSA
                     PSAPPAPPAPPAPPAPPTPPTPSA"
BASE COUNT         1599 a         3451 c         3502 g         1425 t
ORIGIN      
        1 ctacacgatc gccgtctggc gcggcggcct tcccgacgat ccgccgagcg cccgcgtcct
       61 ggttcggcga ttcagctggc tcaccgccga ggtcacggtc gaagacgtca ccatcgacga
      121 gctcgcgctg cgtctcgttc ccggctcggt cacgaccatt cgcttccagg ccgagctgcc
      181 ggtcaccggc gcgttcgaca tcttgctcgc gtcggcgggc atgcgggtcc ggcagtgggt
      241 tgccaccggc cccgcagtcg cctttctggg cgagagcgcg gccgagacaa cctacggacc
      301 cggccagctc gccggcggcc agaccggcct ggtgaccgaa gccttgtggg gcattcgcgg
      361 ccacggcctg gttcgcgccc ggtagcgtca ggccgcaccg tttccgtttc gtcccccttt
      421 cgcgggcccg cggggctctt gctagaatct cccgccgcat tttttggttc tttttcggag
      481 gtgcagaagt ggagctcacg ctagaagccg agatccggca gcaggccggc aagggggtcg
      541 cccgcaagct gcgggccgcc ggcaaggttc cgggtgtcgt ctacggcgcc ggcgaggccg
      601 cgaccgcgat cgcggtcgac cgccgtcagc tgacccacgt cctcgccacc gatgccgggc
      661 tgaacgtgct gatcgacctc aaggtcggca gcgatacgta cctgacgctc gcccgcgagc
      721 tgcagcgcga cgcgctgcgc ggcgacatca agcacgtgga cttcgtcaag atcgaccgca
      781 acaccgcgat cgaggttgac attccgatcc acgttgaagg cgactcgatc ggcgtgcgtg
      841 aaggcggcgt ggtcgagcac cacatctggt cgatcaaggt ctcctgcaag cccggcaacg
      901 tgcccgagtc gatcacggcc gacatcacca gcgtcggcct gcacgagcac ttcaaggccg
      961 gcgatctgac catgcccgcg ggcgtcgagc ttctcaccga ccccgaggaa aacatcatca
     1021 cgatcgccac gccgcaggtg ctcaaggtcg aagccgagct cgagcagccc gggatggagc
     1081 cggccgccgc cgcaggcgaa gccgctccgg ccgaaagcga agaggctccc gccgccgagg
     1141 gcggcgaaga gtcctagaac gccgtgcccc tgtggggccg gcaacgccga cgacccgagg
     1201 agatggcggg cgaggtctgg ctcgtcgcgg gcttgggcaa ccccggcgcc gaatacgagc
     1261 gcacccggca caacgtcggc tttcgcgtgc tcgaccggct ggcgtccgag ctgggtgacg
     1321 cgcggttcaa gcggggcaag gcccccgcca tggtcgccga gacccgtgac ggcgacgtcc
     1381 gcgtgatcct ggtcaagccg gccacgtaca tgaacctgtc cggccaggcg gtcgggccgc
     1441 tcgccacgta ctacaaggcg cccgctgcgt gcgtggtggc cgtgcacgac gagatcgacc
     1501 tgccgttcgg gcggctccag gtcaagatcg gcgggggatc ggccggccac aacggaatca
     1561 gatcgctggt gtcctcgctg ggcaccaacg agttcgtgcg cctgcggatc ggggtcggcc
     1621 ggcccggagg ccgcaaggag gccgccgacc acgtcctgga cacgttcagc aaagcggagg
     1681 agaaggaact gcccgtcgtg gtggccgaag cggccgatgc cgtcctttcg atcttgaggg
     1741 acgggcccga gcgcgcccag aacgccgtga acacgaggaa ttagaaaaca tttctccttc
     1801 gcggtggacc gagcgccgaa tatggcacag actgtgtgaa ggcggatccg cttatccggg
     1861 gggataggga tgaaagacaa gtccgagatc gcggccatag ccgtgatcgt gctcggagtc
     1921 gcgctgatcg cggggctcgt ggtcaacgag aatcggaaag gcgagaccga cgtgctcggg
     1981 actcgcttcg cttcgccgac gccgaccgcg gtcgcgacgg gtgatccgtt cgtgatcccc
     2041 acgatcgact ccacgatcgt tccgacgccg actcccacgc cgaccgtggc gcccgcgacg
     2101 ccgacgccga cggcgacggc gacgacgacc gcgcggccgg cgaccacggc cacgacgatc
     2161 gcgcggccgg cgaccgtggc cgcatatttg gtcgaggttc gatgcggcga aacggtgatg
     2221 tactccgacg ggacgggtgt cggaggcagc ggcggctcct ggcgggagac cgcagccgcc
     2281 ggcaaacgga ccagctacga attcaactac cagcgtgagc agtcgggccg gcaggtgacc
     2341 tcgcagcgca agacctgcga aatcaagacc gagcgcacgc gcgccggcag cgagccgagc
     2401 cttcgcgtga cgcttgcctg cgacgggcgc ggcacctacg agacgtcgcc gccgccgtca
     2461 ccgagcacga acgtcatctc gcgcacgtgg cggctgtccg agctgcagtc cggcttcacg
     2521 cacgagatgc gaaacaagcc gggacaggtc agtgagcaga ccacctgcga ggttcgcgtc
     2581 gtgtagcgag cagtactttc gagcgagacc ggccttcgcg gccggtctcg tcgttttccg
     2641 gcttcgcgcc gaatccggcg gcgatagact gcggtccggc cccgggtcgc gtgatccggg
     2701 gctcgatgcg cgtgcctgat ggatctttcc cgcgtccttt ccgatgtcgc ggtctcgccg
     2761 gagctggccg aggccgtgga tgcgctcgcc ctccggcagg ccgtcaccgt cgcggatcag
     2821 gcccgcaccg tgctgccggc ggtctggtcg ctggaccccg cgcggccgcc gatgctgctg
     2881 atcgccccgt cgtcgcgcga tgccgaacgc atcacggccg agatcaacgc gtggcgctcc
     2941 ggcggcgcgg ttctgttccc ggcgtgggag acgctgccgc acgagcgcct gtcgccgcgc
     3001 gccgagacga tcggccgccg cctcgaggcg ctgtggcggc tgcgcgtgcc acccgaggac
     3061 ggcgtggcgc ccaaggtgat cgtggcgccg gtgcgcgccg cggtgcagcg catggttccc
     3121 ggcgtcgagt cgctgagccc gatcatcgtg cgcaccggcg aatggctcga cgtgcaggat
     3181 ctggttcgcc gtctcgccgg cgcgcagtac gagcgtgtgg acatggtcgg ccggcgcggc
     3241 gagttcgcgg tgcgcggcgg cctggtggat gtgtttcccg cgacggcgga tcatcccgtg
     3301 cggctcgact tcttcggcga cgaggtcact tcgatgcgcg agttcgcggt gtcgtcgcag
     3361 cggtcgatcc gtgacgtcga gcgcatcgtg gcgttctcgt gccgcgagct gatcccgacc
     3421 gacgaggtgc gcggccgcgc gattgccgcg ctcgagcgtg cgccgcacct gcgtcccgag
     3481 ctgatgcgcc tggccgacgg gatcaccttc gaaggcatgg aagcgctgat tgaggtcctg
     3541 tgggacacgc tgccggtcct gcgcgagatt ttgccggacg acacgctcac ggttgtgctc
     3601 gagcccaagc gcaccgccga ccgcgcggcc gagatgctgc aagaggtcga ggcgatgcgc
     3661 gaagcgatgt gggcggccgc cgccgacggc gcccgcccgc cggccgacaa cacctacgcc
     3721 gagctcgacg ccgcgatcgg accggagccg cgcgcggcgc tgtcgccgtt ccggggcgac
     3781 ggtcccgcgc tcgacgcgaa gccgtgggag acgcgcgaca tcgacgcgct cgccgccgag
     3841 gtgcggcggc tgctgcaggg cggatcgcgc gtgatcgtgg ccgcggagtc acacggctcc
     3901 gccgaccgga tccgcgagct gctcgcgggt cacgagctgt tcctgccccg ctacgacggc
     3961 ggtgagcttc cgcccgcccg cggcgcggtg accgttgcgc cgatcgacga aggattcgtg
     4021 tcggagtcgc tcgggcttgc cgtggtcggc gagcacgacc tgttcggaaa gcggcgtccg
     4081 catcgcgagc cgaaggcgaa gcggcgcagc atcatcgcgc tcgagctcga gccgggcgat
     4141 ctggtcgtgc acgcgattca cggcatcgga cggttcaccg gcatggtcac ccgcgaagtc
     4201 ggcaccggca agaccgacta tctggtactc gagtacgcgg cgggcgacac cctctacgtt
     4261 cccgcggaca cgctcgatct ggttaccaag tacgtcggcg gcgagccccc caagctgcat
     4321 cgcctcggcg gcaaggactg ggacaagcag aaggcccgtg tcaagaaagc cgtgcgcgac
     4381 atggcggacg agctcatcaa gctctacagc cagcgcgccg ccgcgcacgg gcacgcgttc
     4441 ggccaggaca cgccgtggca gcgcgagctc gaggacacgt tcccgtacat cgagaccaag
     4501 gatcagctcg ccgcgatcga ggacgtcaag gccgacatgg agcggcccgc gccgatggac
     4561 cggttggtgt gcggcgacgt cggcttcggc aagaccgaga tcgcgatccg cgcggcattc
     4621 aaggccgtcg ccgacggaaa gcaggtcgcc gtgctcgtgc cgaccacgct gctggcgcag
     4681 cagcactacg cgaccttcag cgaacgctat gcgccgtttc cggtgcgcgt cgcggtgctg
     4741 tcgcgcttcg tcacccccaa ggagcagaag agggtcatcg acgagatcgc cgcgggaaag
     4801 atcgacgtgg tcatcggcac ccatcgcctg ctgaacaagg acgtcacctt cgcggacctc
     4861 gggcttttga tcgtggacga ggagcagcgc ttcggcgtcg cgcacaagga ggcgatcaag
     4921 aagctcaaga ccaacgtcga cgtgctgacg atgaccgcga cgccgatccc gcgcacgctc
     4981 gagatgtcga tgtcggggat ccgggacctg tcggtgatcg acacgccgcc cgaagaccgg
     5041 catcccgtgc tcactttcgt cggcccgttc gacgagccca ccgtcgcggc cgcgatccgc
     5101 cgcgagatgc tccgcgaagg tcaggtcttc tacgtgcaca accgcgtgca cgacatcgag
     5161 cgctcgacgc agacgatccg caagctcgtg cccgaggcgc gcatcggcgt cgcgcacggc
     5221 cagatgagcg aacgccagct cgaaaagatc atgctggatt tctgggaccg cgaatacgac
     5281 gtgctcgtgt cgaccacgat catcgagtcc ggcctggaca tcccgacggc gaacacgatc
     5341 atcgtcgacc gcgcggatca gctcgggctg tcgcagatgt atcagcttcg cggccgcgtc
     5401 gggcgggccc gcgagcgcgc ctacgcctac ttcttcttcc cgcccgaacg gtccctgacc
     5461 gagcagtcgc acgcgcggct gagcgcgctg agccagcaca ccgagctcgg aagcgggctg
     5521 cagatcgcgc ttcgcgacct cgagatccgc ggcgccggca acttgctggg cggcgcgcag
     5581 cacggccaca tcaccagtgt cggcttcgac ctgtacgtgc agatgctcgc cgatgcggtg
     5641 gacgaggcgc gcggcaagct gcccgagccc aagccagacg tgaaggtgga catcgcggtg
     5701 gacgcgcacc tgcccgaatc ctggattccg cgcgaaggct tgcggctcga ggcctaccgg
     5761 cgcgtcgcgc aggcgccgag ccacgaggcg ctggccgacg tgcgcgccga gcttttggac
     5821 cgctacggcg agctgccccc gcaggccgag acgctcctga ccgttgcctc gatcaagctc
     5881 gacctcgggc tccgcggcgt tcgcaccgtc tccgccggcg gcggcaaggc ccgcttcgag
     5941 ccgttcgtgc tcgccgagtc cgagcaggtg cgcgtccgcc gcctgttccc cggctcggtg
     6001 tacaagacga acacctacac gctgatcctg ccgatgccgg ccgccgagac tccggctgcg
     6061 tggctgcggc gggccgtgtc cgatatcctc gcgccgtgat gcgaatccgg ctggtcctgc
     6121 ctctgctcgc gctcgcgttg gccgcgagcg catgcggccg ctacttggag gcgtccgcgg
     6181 ccgtggtgga cggcaagccc atctcgatgg ccgatctcga cgccgccgtt ctcgaggcgc
     6241 tccgcggcca ggacgacgtc acccaagaac agcggctcga gacggagcgc cgggttctcg
     6301 ggcagataat ccaggaccgg atcatcgccg gtgaggccga gatccgaaag atcaaggtcg
     6361 acaccgcggc cgtcgaggag cgctatcagg gcatccgcag ccagttcgac agcgacgagg
     6421 agctcgccca ggcgctggcc gcccaaggcg tggacatcgc gaccgtgcgg gaccgcatcc
     6481 gcctgcgact catcgtcgaa gcgatccaga aggagctcgc ggacacggtc aagatcacgc
     6541 ccgcgcagat caaggaagcc ttcggcgacg gctccgcctt ccaggaggtt cgggcccgtc
     6601 acatcttctt tgccgtgccg gatccgtccg ccgagcagac ggcgctcaag aaggcgcagg
     6661 acgcgctcgc acgtctgcgg gccggagcgg acttcgcggc gctcgcgcgg cagatctcgg
     6721 aggatccccg gaccaaggcg tcgggcggcg agctcggggt gcttcgtccc ggcgcggccc
     6781 aagcagggga tctggagcgg gcagcgttcc ggctcgagcg cggcgagatc agcgagccgc
     6841 ttcgctcgca ggccggcttc catctgatcc aggtaaccgg caagggaaag aagaccttgg
     6901 agcaggcgac gccggagatc aaggaggcgc tgcagtccca gggcgcgcag gaggcgttca
     6961 ccgaattcat caccacgcgc atccgcgcgg cccgggtcga ggtgaacccg cgattcggtg
     7021 agttcgatcc cgcccaggtc accatcgtgg atcgccagtt ctacaccccg gctccggtgg
     7081 atcccaacgg cgtgccgggc ctgcagctgc cgctcgacga ggtctccggc tagatctcga
     7141 gccggtaggc gatggccacg aggtcgggca gctcgttcgg ctcgggcacg aagtcgaact
     7201 cgggcgcgcg aacgaagccc atgcgctcgt acatgtcgcg cgccacccac atcagatagg
     7261 acgtgtgcag cccgatcgtg tgcgcgccca gatccttcgc gcggcgaatg cattcgtcgg
     7321 tcaggagccg gccgatgccc aggccccgcg ccgccggatc gaccgacagc agccggacct
     7381 cggtgaatcc ctcgggccag ccgagatgct cggacttggt cgcgtcggga tagaaggtca
     7441 ccgccccgca caacgcgttc tcgtgctcgg cgacgatcag ctgcgagaac ggcatgcggc
     7501 ggcgaacgtc ggcgatctcg gcgcggtagt cctcccacag gtgcgccggc agctcggcct
     7561 cgtattggcc gtaggccgtg accatcaacg ccgagacgtc gtcgagctcg acgttgcggg
     7621 catcgcggat gcgcacggtc atgcgagctt cgtgcggtag gcgatcacga cgaaggtgtc
     7681 ggagacgggg atgtcgttgt cgggaaaccg ttcgaagccc aagcgctcgt acatgccgcg
     7741 ggcgaccgac atcaggtgcg tggtgtgcag cccgaaccat tccgcgccct gctcgcgggc
     7801 gcgtgccagg cacgcctcgg tcaagcgccg tccgatcccg cggccgcgcg cccgtgggtg
     7861 gacgcccagc agccgcatcg ccgcgtagcc ctcgggccag cggtcctcgg ccatgcgctc
     7921 gtcggccgcg tccgggatgt agcacgcggt gccgatcacg gcatcgtcct cgacgacgat
     7981 cagatgcgac gtgaacggcc agcggccgcg tacgtcgggg atctcgtgat cgtagtagtc
     8041 gtcccacgac ggccggtcgg agtcgccggg cggcggagcg aactccgcgt acgccagtcg
     8101 aagcagcgac gcgatctcgt cgatgcgatc gtcggttgcg accaccacat ccatctcgat
     8161 cggcgagcgt accaccggca gccggtatgc tgccgccgaa atgatcggca ttctgggggc
     8221 cggagcgatg ggtgcggcgc tcgccgcgca tctcgcccgc gagggtcgcg acgtggtgct
     8281 gtgcgccacg gaattcgacg agcgcgccgc tcacgccgtc gagcacgcct tgccgcatcc
     8341 gggcctcggc atcgccttgg gcgacggcat ccgcgtcgtg cgttccccgc agtggcccga
     8401 ggccatcgcg tcgatgcgga tcatcaccgt cgcagtctcc accgacgggc tcgccgccgt
     8461 gatcgaagct gcggcctcac acgccgcccc cgacgcgctt tgggcgatcg cgaccaaggg
     8521 gtgggaggag tccacgctgc gcagcagcgc caccgtggcc tgcgacacgc tcggggacgc
     8581 caagcgcgtc gttcagctcg tgggaccgtc gctggccgcg gaggtcgcgg cgggagttcc
     8641 gaccggcatc gtcgcggccg gggtccactt gcctgcggcg atcgcggtcg cggaagcctt
     8701 cgcatccccg ctgctgcacg tgcgagcgac caccgatgtc gccggggtcg agatcggctc
     8761 ggccctcaag aacgtcatcg cgatcgccct gggactctgc gacggcatcg ccgagcacga
     8821 agggcggccg tggaccaaca caaaggccat gctgttctcg cgcgggctgt ccgagatgcg
     8881 cgagctggtg cgggtcagcg gcggacgggg gtccaccgtg ctcggcctgg ccggctcggg
     8941 cgatctgtat gtgacggccc tcggcggtcg caacgcccgc ttcggccgct tggtgggatt
     9001 gggtacgccg cccgaaaagg cgctcgagga gatgaacacc accgtcgagg ggtacgcgaa
     9061 cgcgcccgcg gccgcggcgc tggccgccaa gcacggcctg gacctggcga tcgtgcgcgc
     9121 cgtgaacgcc gtgcttttcg aagggctgcc gccggcggag gcgatccgct cactgtcggg
     9181 tggtgtcggc gtcgacgagt agcacatgcg tcgttgcagg tgacaagcct tcatcgtagg
     9241 ctcgcacgaa gatgaatcgc ggtgcgcgcg gccacgagaa cgattccggc gagacgttcg
     9301 acgcgagcga cgctgccgag gtggatgacg acgccatgat ccgcgagccg gttcacgagg
     9361 atcgcgccga catcgaggag ccggcccgcg atctccacga agacaccccc accgccgagc
     9421 ggctcagccg tgtcgccgaa gggctgtctc gcgtcgccga ggagctcggg cggggcagcg
     9481 agcatctgcg cgccaagccc gaaccgcgga aagagccgcg gaaagagccg cggaaagagc
     9541 cgccgaaaga gccgggaaag gagcaggcga aggaagcggt gaagcgccgg tcgaaggaag
     9601 cggcgaagga agcggccaag gaagcggcca aggaagcggc gccggccgcc tcgtccggcg
     9661 cgggtgccgg taacgtcgcc ggcggcgatt cgggagccgg ggacgtggaa agcgaagacg
     9721 gtatgagcgc acccacgatc catccagccc ccgaggccgc gccgccggcc gaggaaacgc
     9781 ctaccgtcga actcgccgac atggagcgaa gcgaggagcc cggcatcgtt ccacccgccg
     9841 ccgtcgatgc gccgacggtc gagttgacgg cggagcagga tccggcgcca tccgcgccat
     9901 ccgcgccatc cgcgccaccc gcgccacccg cgccacccgc gccacccgcg ccacccacgc
     9961 cacccacgcc atccgcg
//
LOCUS       DATLFO010000178         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_56453, whole genome shotgun sequence.
ACCESSION   DATLFO010000178 DATLFO010000000
VERSION     DATLFO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56453"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..888)
                     /locus_tag="VM841_07155"
     CDS             complement(55..888)
                     /locus_tag="VM841_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HVL89994.1"
                     /translation="MAEREEVNIDQATFDKAIAEGKPERVARALAKSAYVRKTKGPAG
                     PGVKKEAAAAAPAEGAPAAAAAGAPAASAAPAATAVAERLTPEQRAARVAAATGRTSG
                     GNGGGGRGLSGEQVHRLLAVVPPEGIQRTVKRQDDKVNVWPHLLSAEFLSLLLCTAFV
                     LVFSTFVHAPLRFLANPSLTPNPSKAPWYFLGLQELLRYFHPMVAGVTIPGLGLFGLM
                     ALPYADKNPSMQPSRRKLAIILFTMFMMFWTVLVVIGVAFRGPGYNWIWPWDSGLYFD
                     L"
     gene            complement(898..1578)
                     /gene="extP"
                     /locus_tag="VM841_07160"
     CDS             complement(898..1578)
                     /gene="extP"
                     /locus_tag="VM841_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HVL89995.1"
                     /translation="MRKGYKDTSRDRALATMNNVLYHLHPTKVRRHGLKLTYTYCLGG
                     LSFFLFVILTITGIYLMFFYRPAVDLTSAPVYQDMQDLSQAITFGQLVRNMHRWAAHL
                     MVLSVFLHMARVFYHGAYKPPREFNWVVGVNLLQITLLLSFTGYLLPWDQLAIWAVTV
                     GTNMMGYTPVFGRQVKFVLVGGLEVGPPTLIRWYVLHVLALPFVAVLLMAVHFWRIRK
                     DGGISGPL"
     gene            complement(1703..2056)
                     /locus_tag="VM841_07165"
     CDS             complement(1703..2056)
                     /locus_tag="VM841_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVL89996.1"
                     /translation="MSAISNKPEFYDEYDLKIVDPNWLQERVKPKRHIGLEADLCILC
                     RACEDVCPWDCIYMLSGEIVADADQAAIKDAAGNSAAVFVIDDTECTRCGICVERCPS
                     DALFYITVGKGKAQA"
     gene            complement(2053..>2414)
                     /locus_tag="VM841_07170"
     CDS             complement(2053..>2414)
                     /locus_tag="VM841_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89997.1"
                     /translation="EIIWFGAAAAMFLAAWKTKSDWVRASLAGFALSILAIRVLAVLP
                     SWWLYYAEGTLDWGGQGCAEIDLQCIKQTIKDSVVVVQNAVILGALVVGFIIWQKKFP
                     KQLAPGESKPEATGGYK"
BASE COUNT          448 a          778 c          771 g          417 t
ORIGIN      
        1 gatgatcgcg atcccgatcc actgcgctgt cgatactgcg agcatggtca acacctacag
       61 gtcgaagtag aggcccgagt cccacggcca gatccagttg tagcccggac cgcggaatgc
      121 gacgccgatg acgacgagga ccgtccagaa catcatgaac atcgtgaaca agatgatcgc
      181 gagcttgcga cggctcggct gcatgctcgg gttcttgtcc gcatacggca gcgccatcag
      241 cccgaacagg ccgagcccag gaatcgttac gcctgcaacc atcgggtgga agtaccgcaa
      301 cagctcctgc aaaccgagga agtaccacgg tgccttcgat gggttcggcg tcagcgacgg
      361 attcgcgagg aagcgcagcg gcgcgtggac gaacgtcgag aagacgagca cgaacgccgt
      421 gcacagcaga agcgacaaga actccgccga gagcaggtgc ggccacacgt tgaccttgtc
      481 gtcctgacgc ttgaccgtgc gctggatccc ttcgggcgga accacggcga gcaaccggtg
      541 gacctgctct ccgctgagac cacgcccgcc gccgccgttg cctccggagg tacggccggt
      601 cgccgctgcg acgcgcgcgg cgcgttgctc gggcgtcagg cgttcggcga ccgcggtggc
      661 tgccggcgcg gcgcttgccg ccggagcacc cgcggcagcg gctgcgggtg ctccctcggc
      721 gggcgctgcc gctgcggctt ccttcttcac gccggggccg gccggcccct tcgtcttgcg
      781 gacgtacgcc gacttggcca gcgcgcgcgc aacgcgttcg ggtttcccct cggcgatggc
      841 cttgtcgaac gttgcctgat cgatgtttac ttcttcgcgt tccgccatcg cagcctccta
      901 cagcggcccg ctgatgccgc cgtccttacg gatgcgccag aagtgcacgg ccatcagcaa
      961 gacggcgacg aacggtaagg ccaggacatg gagtacgtac catcggatca gtgtcggggg
     1021 tccgacctct aaccctccga cgagaacgaa tttcacctgc cgcccgaaga ccggcgtata
     1081 acccatcatg ttcgtgccga cggtgacggc ccagatcgcg agctgatccc aggggagcag
     1141 gtatccggtg aacgaaagca gcagcgtgat ctgcagcagg ttgacgccga cgacccagtt
     1201 gaactcgcgg ggcggcttgt atgcgccgtg gtagaacacc cgcgccatgt gcaggaacac
     1261 gctgagcacc atcaagtgcg ccgcccaccg gtgcatgttg cggacgagtt gcccgaaggt
     1321 gatggcctgc gagaggtcct gcatgtcttg gtagaccggc gccgaggtca gatccactgc
     1381 gggccggtag aagaacatca ggtagatgcc ggtgatcgtc aggatcacga aaaggaagaa
     1441 agaaagaccg ccgaggcagt aggtgtaggt cagcttcaac ccgtggcggc gcaccttcgt
     1501 ggggtgcagg tggtacaaga cgttgttcat cgtcgccagc gcgcgatcac gcgaggtgtc
     1561 cttgtatccc ttgcgcatga aggacccagg acggaagatc gacttccata cctgcgagtc
     1621 ctgcgtcttg tcccgcagga gctctaactt ctcctggagg agcggcttct ccgtcttcgc
     1681 cacgttcggt cctccgcccg gcctaggcct gggctttccc cttgccgaca gtgatgtaga
     1741 agagcgcgtc cgacgggcac cgctcgacgc agatcccgca gcgcgtgcat tcggtgtcgt
     1801 cgatcacgaa caccgcggcc gaattgccgg ccgcatcctt gatggcagct tgatcggcat
     1861 cggcgacaat ctcacccgag agcatgtaga tgcagtccca cgggcagacg tcctcgcacg
     1921 cgcggcacaa gatgcagagg tcggcctcga ggccgatgtg gcgcttcggc ttgacacgtt
     1981 cctgcagcca gttcgggtcg acgatcttga ggtcgtactc gtcgtagaac tcgggcttgt
     2041 tggagatcgc gctcacttgt acccacctgt cgcttcaggc ttggattcgc cgggcgccag
     2101 ctgcttgggg aacttcttct gccagatgat gaatccgacg acgagggccc cgaggatcac
     2161 ggcgttctgc acgacgacga ccgagtcctt gatcgtctgc ttgatgcact ggaggtcgat
     2221 ctccgcgcaa ccctgcccgc cccagtccag cgtgccttcg gcgtagtaca gccaccagct
     2281 cggaaggacc gccaggacgc ggatcgccaa gatcgaaagc gcgaagccgg cgagggaggc
     2341 gcgtacccag tcgctcttgg tcttccatgc cgccaggaac atcgccgcgg ccgccccgaa
     2401 ccagatgatc tcga
//
LOCUS       DATLFO010000179         4901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_141784, whole genome shotgun sequence.
ACCESSION   DATLFO010000179 DATLFO010000000
VERSION     DATLFO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141784"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..936)
                     /locus_tag="VM841_07175"
     CDS             complement(100..936)
                     /locus_tag="VM841_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL89998.1"
                     /translation="MHSIAARRPVALAGAAAAVVYVIAASVSGALSPLARLPLLDGVH
                     NTAPYSWVNTPPGEQPGDRDPVSINERLDLPAAGNVETIIATGDDQVSVKLPDAAIKP
                     AEGRRQAEVQVEPFDHTTYTAPAGYVIRGNVYRVGIREVPSGKGIPTIEGSGQITMTY
                     AAPTQELLNASHDLMFSSDGQDWRVLNAQDQHATLSVTAPFIDIGYFAAVAKIPEDDL
                     LEEEEGGGFPWLILVVVAVAAGAGFAVTLRLRKARAEARRREQIRRELARQSRSTKKR
                     RR"
     gene            complement(953..1543)
                     /locus_tag="VM841_07180"
     CDS             complement(953..1543)
                     /locus_tag="VM841_07180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVL89999.1"
                     /translation="MACRPMIAAAPAAPSRGVTLAIAIALGASLFVVRPLTVLDPRAA
                     PLLLTGIYAALWAVSVAPAVPSYAAATASRTMSWRAVTLIGLVAVVIVRFTADPVVRP
                     DRRLVAIALGLAAAVAEEAYFRRLLHPVLARAGAAVAIVAGALLFAAMHYQSYGWAAM
                     PLDFGAGLLFAWQRWASGGWSAPAATHGAANLLASI"
     gene            complement(1525..2007)
                     /locus_tag="VM841_07185"
     CDS             complement(1525..2007)
                     /locus_tag="VM841_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90000.1"
                     /translation="MEHVLIRGLEHLAGSKGAPRLGYAVEMRDRKGPAHKHGAFEDDG
                     VWVQIHGGLFVAKAKVKICWIGEYSRVEDVRRRTAGAPIHDDDAFWTGRPRFGYAAVA
                     ELKHETWVPPFWAGPRTYGYEWVVLDNDSKRASWLDRKDPPRGGESLAAAFREWLAAR
                     "
     gene            complement(2007..2687)
                     /locus_tag="VM841_07190"
     CDS             complement(2007..2687)
                     /locus_tag="VM841_07190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVL90001.1"
                     /translation="MDAPKTLEADGLPRRLDEVAPAGGGLVARIARTARFIKRNRLYK
                     LRYVMMALRFVWFKIRNPHILTDGFFFLERGAEVYARKGYGRLRIGRWVWVGTGNALR
                     CHEGNLTIGDKCVFGRKNTINAYMDVTIGADCIFADWIYITDFDHKFDDLSMPIRKQG
                     IVKSPVKIGEDCWFGEKTTVLRGVTVGRGCVIASHALVNRDLPPLSVAGGVPARVLKS
                     RAHGQKKA"
     gene            complement(2720..3634)
                     /locus_tag="VM841_07195"
     CDS             complement(2720..3634)
                     /locus_tag="VM841_07195"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90002.1"
                     /translation="MTNLDPAAMDAKTANVLYHDWEAQNYDDRWSISFDERCVSYARE
                     KFGRVAPIQKYGDVLEIGCGTGFFIINLWQAGLVERAYATDISPGMVEACRRNAERVG
                     LTDLEGKASDAESLPYEDNSFDLVVGHAVLHHIPDVPAALKECFRVLRPGGRLVIAGE
                     PTRIGYQIVLRARRGTAAVVKAIAKVKPDIRLPEPDHSGLAPEAALEAHVDLHEFHPH
                     MVRGWLRDAGFGTVNVRTEEFLSGLLGWSNRTIEAMVRPELLGKSWPFFAYHSYMAMY
                     RLDEWVVRRIVPKPLFYNVLLSATKPGD"
     gene            complement(3682..>4901)
                     /locus_tag="VM841_07200"
     CDS             complement(3682..>4901)
                     /locus_tag="VM841_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL90003.1"
                     /translation="AASAFDVPRDSGPLAAAVRASLWARVFVSTGGLVASAFLPAGRP
                     ARVWFSVLVAVVAIPYTLTVLIATHRGRMRGVSPVTFAGDVAVVALFQAAFVESRPTM
                     YTVYLVLVCFYTVVAGMGWGMSVAVFGAAADLILQVADPNPAGLNGFAFVSYLAAAAA
                     LGIIVSAATKHEREGMRRRVSTVSHELRGPVTSIEGFAATLSERWTEMEERQRIEYIR
                     RIRRNAASVHSIIETFLDAADVGGGRFRPAIAVVPLRSFVDDLCRLCAPLVLGREVRI
                     DVPAEIHVAADPTLLERILINLVMNAHGYSPAGEAILLEGRMAGERVEISVTDRGPGI
                     PQPVREQIFESFWRPAGQGRRGSGIGLSLARDLVKAMDGRIWVESAPGGGSRFAVALP
                     AAIPEPGSERRAV"
BASE COUNT          822 a         1698 c         1682 g          699 t
ORIGIN      
        1 agaggaggcc cttcgccgcg gcctcggcag cgacgaccgg ccgccgctgc gcgcgatgct
       61 cggccggtcg ccgctgctgg gcctggcgga cggcagcgcc tagcggcgcc gcttcttcgt
      121 cgatcgcgac tgacgggcga gctcgcggcg aatctgctcc cggcgccgcg cctccgcacg
      181 tgccttgcgc agacgaagcg tgacggcgaa cccggcgccg gccgcgacgg ccacgacgac
      241 gaggatgagc caggggaaac cgccgccttc ttcctcctct agaagatcgt cctcgggaat
      301 cttggccacc gccgcgaagt aaccgatgtc gatgaagggc gcggtgacgg acagcgtggc
      361 gtgctgatcc tgcgcgttga gcacgcgcca gtcttgaccg tcggacgaga acatcaagtc
      421 gtgcgaggcg ttgagcagct cttgcgtcgg cgccgcgtag gtcatggtga tctgcccgga
      481 gccttcgatg gtcgggattc ccttgccact ggggacctca cggatcccga cgcgatagac
      541 gttgccgcga atcacgtatc ccgcgggggc ggtatacgtg gtgtgatcga acggctcgac
      601 ctgaacctcg gcctgccgcc ggccttcggc cggcttgatc gcggcatccg gcagcttgac
      661 cgagacctgg tcgtcgccgg tcgcgatgat cgtctcgacg ttgccggcgg cgggaaggtc
      721 gagacgttcg ttgatcgaga ccgggtcgcg gtcgccgggc tgctccccgg gcggggtgtt
      781 gacccagcta tacggagcgg tgttgtgcac gccgtcgagc agcgggaggc gcgcgagcgg
      841 actgagcgct cccgagacgg aagcggcgat cacgtagacg accgcggcgg cggccccggc
      901 cagcgcgacg ggccggcgag cagctatgga atgcaacgcg tttcgctcct ttctagatcg
      961 atgcaagcag gttggcggcg ccgtgggtcg ccgccggggc ggaccatccg cccgacgccc
     1021 agcgctgcca cgcgaacagc agcccggcgc cgaagtcgag cggcatcgcg gcccatccgt
     1081 aggactggta gtgcatcgcg gcgaacagca gggctccggc cacgatcgcg acggccgcgc
     1141 cggctcgggc gagcaccgga tgcagcaggc ggcggaaata ggcctcctcg gcgaccgctg
     1201 cggcgaggcc gagagcgatc gccaccaacc gccggtccgg ccgcacgacc gggtcggcgg
     1261 taaaccgcac gatcacgacc gccacgagtc cgatgagggt cacggcccgc caagacatcg
     1321 tgcgcgaggc ggtcgcggcc gcgtaggacg ggaccgccgg ggcgaccgac accgcccaca
     1381 gcgcggcata gatgcccgta aggagcagcg gggccgcacg cggatccagg accgtgagcg
     1441 gccgcacgac gaacagcgac gcacccagcg cgatcgcgat cgccagtgtg accccgcgtg
     1501 aaggcgcggc gggcgcggcg gcgatcatcg ggcggcaagc cattcccgga atgcagcggc
     1561 cagggactcg cccccgcggg gcggatcctt gcggtccagc cacgacgccc gcttggagtc
     1621 gttgtcgagc acgacccatt catagccgta ggtgcgaggc cccgcccaga agggcggaac
     1681 ccaggtctcg tgcttgagct cggcgaccgc cgcgtaaccg aagcggggcc ggcccgtcca
     1741 gaaggcgtcg tcgtcgtgga tcggcgctcc tgccgtgcgt cgccgcacgt cctccacccg
     1801 cgagtattcg ccgatccagc agatcttgac cttggccttg gcgacgaata gcccgccgtg
     1861 aatctgaacc cagacgccgt cgtcttcgaa cgcgccgtgc ttgtgtgcgg ggcccttgcg
     1921 atcgcgcatc tcgacggcat agccgagccg tggcgcgcct ttgctgccgg ccaggtgctc
     1981 gagaccacga atcagaacgt gctccatcac gccttcttct gaccgtgggc ccgggacttg
     2041 agcacgcggg ccggaacgcc gcccgcgacc gacagcggcg gcaggtcccg gttgacgagc
     2101 gcgtggctcg cgatcacaca gccccggccg acggtgacgc cgcgcagcac cgtggtcttt
     2161 tcgccgaacc agcagtcctc gccgatcttc accggcgact tcacgatgcc ctgcttgcgg
     2221 atcggcatcg ataggtcgtc gaacttgtgg tcgaagtcgg tgatgtagat ccagtcggcg
     2281 aagatgcagt cggcgccgat ggtgacgtcc atgtaggcgt tgatcgtgtt cttgcggccg
     2341 aacacgcact tgtcgccgat ggtgaggttc ccttcatgac atcgcagcgc gttgcccgtt
     2401 cccacccaga cccagcgccc gatgcgcagc ctcccgtagc ctttgcgcgc atagacctcc
     2461 gcgccccgct ccaggaagaa gaagccgtcg gtcaggatgt gggggttgcg gatcttgaac
     2521 cacacgaacc gcagcgccat catcacgtag cgcagcttgt agagccggtt gcgcttgatg
     2581 aaccgggctg tgcgcgcgat gcgcgcaacg agtccgccgc cggccggggc gacctcgtcg
     2641 aggcggcggg gcagcccgtc cgcttccagc gtcttcggag cgtccatcgg gtcttccggt
     2701 cggcgcgacg agcgcctggc tagtcgcccg gcttcgtcgc gctcagaagc acgttgtaga
     2761 acagcggctt ggggacgatg cggcgcacga cccactcgtc gagccggtac atcgccatgt
     2821 acgagtggta ggcgaagaac ggccacgact tgcccagcag ctccggacgc accatcgcct
     2881 cgatggtccg gttgctccag ccgagcaggc cggacaagaa ctcctcggtg cgcacgttga
     2941 ccgtcccgaa gcccgcgtcg cgcagccagc cgcgcaccat gtgcggatgg aactcgtgca
     3001 agtccacgtg cgcctcgagc gctgcctcgg gagcaagacc cgaatgatcg ggctcgggaa
     3061 gacggatgtc cggcttcacc ttcgcgatcg ccttgaccac ggccgcggtg ccgcggcgcg
     3121 cgcgcagcac gatctgatag ccgatgcgcg tcggctcgcc cgcgatcacc aggcgcccac
     3181 ccgggcgcag gactcggaag cactctttca gcgcggccgg cacatccggg atgtggtgca
     3241 gcaccgcatg gccgacgacg agatcgaagg agttgtcctc gtagggcagc gactcggcat
     3301 cgctcgcctt gccttcaaga tcggtcaggc cgacccgctc ggcgttgcgc cggcacgctt
     3361 cgaccatgcc gggcgagata tcggtcgcgt aggcccgctc gacaaggccg gcctgccaca
     3421 ggttgatgat gaagaacccg gtgccgcagc cgatctcgag cacgtcaccg tacttctgga
     3481 tgggcgcgac acggccgaac ttctcgcggg cgtaggagac gcagcgctca tcgaaggaga
     3541 tgctccagcg atcgtcgtag ttctgcgctt cccaatcgtg atacaggacg ttcgcggtct
     3601 tggcgtccat ggctgccgga tccaggttcg tcatgagcgg caaggataca cgccgtcccc
     3661 gggcggttcc gaatcgcccg cctagaccgc gcgacgctcc gagccgggct cgggaattgc
     3721 ggcgggcagc gccaccgcga agcgggatcc gccgccgggc gcgctctcga cccagatccg
     3781 gccgtccatc gccttaacca ggtcccgggc cagcgacaga ccgatgccgc tgccgcggcg
     3841 gccctgcccg gcaggccgcc agaacgactc gaagatctgc tcgcgcacgg gctgcgggat
     3901 gcccggaccc cggtcggtca ccgagatctc gacccgttcg ccggccatcc ggccttccag
     3961 cagaatggcc tcgccggcgg gcgagtatcc gtgcgcgttc atgactagat tgatcaggat
     4021 ccgctccaac agcgtggggt cggccgcaac gtgaatctcg gcagggacgt cgatgcgaac
     4081 ctcccggccg agcacgagcg gcgcgcacaa gcggcacaga tcgtcgacga acgagcgtaa
     4141 cggcaccacg gcgatcgcgg gcctgaaccg gccgccaccg acgtcggccg catcgaggaa
     4201 cgtctcgatg atgctgtgca ccgaggcggc gttgcggcgg atgcggcgga tgtattcgat
     4261 tcgctgacgt tcctccatct cggtccaccg ctcgctcaag gtcgccgcaa agccctcgat
     4321 ggacgtcacg ggcccgcgaa gctcgtggga caccgtggag acgcgccggc gcatcccttc
     4381 gcgctcgtgc ttggtggccg ccgacacgat gatcccgagc gcggccgcgg ccgccagata
     4441 ggacacgaac gcgaaaccgt tcagcccggc aggattgggg tcggcgacct gaaggatcag
     4501 gtcggccgcc gcgccgaaga cagcgaccga catgccccag cccatgccgg cgaccaccgt
     4561 gtagaagcag acgagcacca gatagaccgt gtacatcgtc ggccgcgatt ccacgaacgc
     4621 cgcctggaac aacgcgacca cggcgacgtc gccggcgaag gtgaccggcg agacgccccg
     4681 catccgtccg cggtgggtgg cgatcaagac cgtcagcgtg tacgggatcg cgacgaccgc
     4741 aaccaggacc gagaaccaaa cgcgagccgg ccgtccggcc ggaaggaacg ccgaggccac
     4801 gagccccccc gtggagacga acacccgagc ccacagcgag gcgcgaaccg cggccgccaa
     4861 agggccgctg tcacgaggca cgtcgaatgc ggatgccgcc g
//
LOCUS       DATLFO010000180         5135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_3738, whole genome shotgun sequence.
ACCESSION   DATLFO010000180 DATLFO010000000
VERSION     DATLFO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3168
                     /locus_tag="VM841_07205"
     CDS             <1..3168
                     /locus_tag="VM841_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90004.1"
                     /translation="HIDFLLTSGPDLIVDNGYDGIVLRDTEATFAIAVRLTGLRRASV
                     STAAPYALTIEKKAGPFLVDLVQGARTTRVTVHNLPDALTLTLDPAGSASYSGSAPIA
                     HVSVRIADPAGVTGRATSVMLDLNELPAELDVSFGAGQTIAVDAHGGSIGLIDLLATS
                     GPALTLPSGYDGVLLDDLASHYAFAVRLSGLRLVSVSTAAPYALDIRKTAGPFLINLA
                     QGQRTTRLDVRDLPDSLQATFDPSGSLSYQASAPIASLTADLVDPAGVAGRATEFHLV
                     VTGLPEALSLSFASGASVSLDAGEASIGSIELVATSGPPIAVDAGYDGIVLNDQQDRY
                     GLAVRLTGLRSASVSTVAPYSLNLSKTAGPFLIRLTQQSRLTHVAVHDLPAIFGATLD
                     PTGRFSYTASAGIGSLTARITDSEPVLGRANRVELDVTDLPSSLTLDFDSSSAVGLDA
                     HGFAIGSISFLATSGPELVVDSGYEGVVMEDVPGHFAIAARLTGLRVARVSTGEAPYT
                     LTLSKNPGPFLARLIQGARRVDVKINDLPSSLTATLNPSGSLVYEASAAVGEITASIV
                     DPAGVAGRATRATALLRGLPASLDITWAGASGAVSADAKGATVGLIEVLLTSGPVPSL
                     PAGRDGFILEDVSSHYAVLGRITGLRKVFFAQGPPPSFNLETAGGRIFQVGVTTQKPE
                     GLATVSAVLDSLPSQVSIAFTSSTALQYRASGVVSRLTIDAVDPVSISGRAKELHGTL
                     LGLPTALDASWAADGTITVDARGGTTELVELQLTSGPDERLAASFDGILMNDLADRYV
                     VFARLTNLQKAIATQSPQPDITINTTGNRVLKVDVNQLKDGKVTYTRATLDRLPATVR
                     VRLLTSPQRVIYTASAATNSLVFQTNSGDRWGLDASLVPLPASVFFCSDGGGGCSNVG
                     RSDAGSFAFDASAYTTINLFDCKRPLNSSCVRGGGASEYVSVDNVNLKKMVFNAGASS
                     SGFEGSTYSDTDWNTVTGHIRLEQSNAIDIDFGVFRADNHRVEWTGWGIWPFTPERSG
                     PVSCNGTSVDIRIIGIWIGITGYVC"
     gene            complement(3225..3659)
                     /locus_tag="VM841_07210"
     CDS             complement(3225..3659)
                     /locus_tag="VM841_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HVL90005.1"
                     /translation="MIAVDTNVLVYAHRKEAIEHDAAFEVVRELSESSDPWAIPWPCV
                     YEFFSVVTNPKIWKNAASSPEQAWKQLEAWFAAPGLRLLAEPDDFAPVLSEFLVRPRV
                     RGAIVHDARVATICVAHGIEKLFTRDRDFSLFPELITENPFG"
     gene            complement(3660..3899)
                     /locus_tag="VM841_07215"
     CDS             complement(3660..3899)
                     /locus_tag="VM841_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HVL90006.1"
                     /translation="MKTTIDIADSLLARAKRHARKVGKPLRAVVEEGLRRVLDAPSEP
                     RGYKLPDMSVGRPGDPNPLEAMSWQDLRDEIYGGR"
     gene            4165..>5135
                     /locus_tag="VM841_07220"
     CDS             4165..>5135
                     /locus_tag="VM841_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL90007.1"
                     /translation="MTDMIRTAGLTKRFGDVTALEGLDLVVPKGTVLGLLGPNGAGKT
                     TAVRILTTLLDPDEGTVEIAGLDAMREPEKVREKIGLSGQSAAVDEHLSGFENLEMVG
                     RLYGLDRRRAKVRASQLMERFDLAFAGKRMSKTYSGGMKRRLDLAAALVAEPEVLFLD
                     EPTTGLDPRSRLALWDEIRTLVRGGSTLLLTTQYLEEADRLADDIVVIDRGRMIAHGT
                     ADELKAKVGGERIEMVLASPEDEGVVRRILEKLSDEEVQYDPDARRFTAAVPDGQEAL
                     RTALTALKRSKTEVRDVGFRRPTMDDVFLTLTGHHVDGDAPVTEQEAA"
BASE COUNT          860 a         1675 c         1690 g          910 t
ORIGIN      
        1 cacatcgatt tccttttgac gtcgggcccg gatctgatcg tcgacaacgg ctacgacggg
       61 atcgtgcttc gcgacacgga agctacgttt gccatcgcgg tacggctgac cggattgcgg
      121 cgcgcgagcg tttcgaccgc agcgccctac gcgctcacga tcgaaaagaa ggcgggaccg
      181 ttcctggtgg atctcgtgca gggtgcgcgc acgactcgag tgacggtcca caacttgccc
      241 gatgcgctga ccctgacgct cgatcccgcc ggcagcgcga gctacagcgg cagcgctccg
      301 atcgcgcacg tctcggtccg catcgccgat ccggcgggcg tgaccggccg ggcgacgtcg
      361 gtgatgctag acctgaacga gctgccggcg gagttggacg tgtcgttcgg cgccggtcag
      421 accatcgcgg tggacgcgca cggcgggagc atcggtttga tcgatctgct cgccacatcg
      481 ggcccggctt tgacgttgcc atccggatac gacggcgttc tgctggatga tctcgcgtcg
      541 cactatgcat tcgcggtccg gctgtcgggg cttcgactgg tcagcgtctc aaccgcggcg
      601 ccgtacgcgc tcgacatccg caagactgcc ggaccgttcc tgatcaacct ggcgcaaggt
      661 cagcgcacga cgcggctcga cgttcgcgat cttcccgact cccttcaggc gaccttcgat
      721 ccgagcggat cgctctcgta ccaggcctcc gcgccgatcg cttcgctgac ggccgacttg
      781 gtggacccgg cgggagtcgc cggacgcgcg accgaattcc acttggtcgt gaccggactg
      841 ccggaggcct tgtcgctgtc gttcgcatcc ggcgcgtcgg tgtcgctgga cgcaggcgaa
      901 gcatcgatcg gctcgatcga gctcgtcgcg acgagcggcc cgccgatcgc cgtcgacgcg
      961 ggctacgacg gcatcgtgct gaacgaccag caggaccgct acggcctggc ggtacggctc
     1021 accggattgc gatctgcgag cgtgtcgacc gtcgcgccgt attctctcaa cctgtccaag
     1081 acggccgggc cgttcctcat ccggctgacg cagcagtcgc gactgaccca cgtcgccgtt
     1141 cacgacctgc cggcgatctt cggcgcgacg cttgatccca ccggacgatt ttcctacacc
     1201 gcctcggccg gcatcgggtc cttgaccgcg aggatcaccg actccgagcc ggtgttgggc
     1261 cgcgccaacc gcgtggagct cgacgttacc gatctgccgt cgtcgctgac gctggacttc
     1321 gactcatcat cggctgtcgg gctcgacgcg catggctttg cgatcggatc gatctcgttc
     1381 ctcgcgacgt cggggccgga actcgtggtc gactccggct acgagggtgt cgtgatggag
     1441 gacgtgcccg ggcatttcgc gatcgccgcc cgtctgaccg gcctgcgggt cgcgcgcgtc
     1501 tcgaccgggg aggccccgta tacgttgacg ttgtccaaga atcccgggcc gttcctcgcg
     1561 cgtctgattc agggagcacg cagggtcgat gtgaagatca acgatcttcc gtcgtccttg
     1621 accgcgactc tgaacccgtc gggcagcctg gtctatgaag cttccgcggc ggtgggggag
     1681 atcaccgctt cgatcgtgga tccggccggg gttgcggggc gagcaacgcg ggcgacggcg
     1741 ctgcttcggg gcctgcccgc gtcgctcgac atcacctggg cgggagcgag cggcgccgtc
     1801 tcggccgatg caaagggtgc cacagttggc ctcatcgagg ttctcctcac cagcggtccg
     1861 gtcccgtcgc tgcccgccgg tcgcgacgga ttcatccttg aggacgtgag cagccactac
     1921 gcggttctcg gccgcatcac gggcttgaga aaggttttct ttgcgcaggg gccgccgccg
     1981 tcgttcaact tggagacggc gggcggtcgc atcttccagg tcggtgtgac gacccaaaag
     2041 cccgagggcc tcgcgacggt ctcggcagtt ctggactcgc tgccttcgca ggtttcgatt
     2101 gccttcacct cgtcgaccgc cttgcaatac cgggcgtcgg gggtcgtgtc gcgactgacg
     2161 atcgacgcgg tggatccggt gagcatctcc ggacgggcca aggagcttca cgggacgctc
     2221 ctgggcctgc cgacggcgct cgacgcgagc tgggcggcgg acggcaccat taccgtggac
     2281 gcgcgcggcg gaaccaccga gctggtggag cttcagctta cctccggacc cgacgagcgc
     2341 cttgccgcgt ccttcgacgg gatcctgatg aacgacctgg cggatcgata cgtcgtgttc
     2401 gcgcggctga cgaacctgca gaaggcgatc gccacgcaat cgccgcaacc cgatatcacg
     2461 atcaacacca cgggcaaccg ggtcttgaag gtggacgtga atcagctcaa ggacggaaaa
     2521 gtcacctaca cgcgcgccac gctggatcga ctgccggcga cggtgcgtgt gcgtttgctg
     2581 acctccccgc agcgcgtgat ctataccgcc tcggcggcca cgaacagtct tgtcttccaa
     2641 accaactcgg gggatcgctg gggcttggac gcctcgctgg tcccgttgcc ggccagcgtg
     2701 ttcttctgtt cggacggcgg cggcgggtgc agcaacgtcg gccgctccga cgcaggatcg
     2761 ttcgcgttcg acgcgagcgc ctacaccacg atcaacctct tcgactgcaa acggccgctg
     2821 aactcgtcgt gcgttcgcgg cggcggggcc agcgaatatg tgtcggtgga caacgtcaac
     2881 ctcaagaaga tggtgttcaa cgctggggcg tccagcagcg gctttgaggg atcgacctac
     2941 agcgataccg actggaacac cgtgacggga cacattcgcc tggagcaatc caacgcgatc
     3001 gacatcgact tcggcgtctt ccgcgccgac aaccatcgag tcgagtggac cggatggggg
     3061 atctggccgt tcacgccgga gcgatcggga ccggtgagct gcaatggaac cagcgtcgac
     3121 atccgcatca tcggaatctg gatcggaatc accggctacg tttgctgagc ggctccgcga
     3181 tcgccaatta cacaggaaat ctgacattcc ccgtaccgga tcgatcagcc gaatgggttc
     3241 tcggtgatga gctccggaaa cagagagaaa tcacggtctc gcgtgaacag cttttcgatt
     3301 ccgtgcgcga cgcagatcgt tgccacgcga gcatcgtgaa cgattgcgcc gcgaacacgc
     3361 gggcgtacca ggaactccga cagcaccggc gcaaaatcat cgggctcggc cagcagacgc
     3421 aggcccgggg cggcgaacca ggcctcgagt tgtttccagg cctgttccgg cgagcttgct
     3481 gcgttcttcc agatcttcgg attggtcacg acgctgaaga actcgtagac gcacggccat
     3541 gggatcgccc acggatcgct gctctccgac agctcgcgca cgacctcgaa tgccgcgtca
     3601 tgttcgatcg cctctttgcg atgcgcgtac acgaggacat tcgtgtcgac cgcaatcact
     3661 cagcgaccgc cgtagatttc gtcccgcagg tcttgccacg acatcgcctc gagcgggttc
     3721 gggtcgcccg ggcgcccgac gctcatgtcc ggaagcttgt atccgcgagg ttcagagggc
     3781 gcgtcgagca cgcggcgcag accctcctcg acgacagcgc gcaatggctt gcccaccttg
     3841 cgggcgtgcc gttttgccct ggcgagcagc gaatccgcga tgtcgatcgt cgtcttcatg
     3901 acgatacagc atacccatat ccatcagtat gggcaagccg tattcgctaa gtaggacgcc
     3961 tacccgctgg ccgccggcac atgcctcgat ctgggacttc aaaccggcga cggacattgt
     4021 cgcatggcgg gaattctggt gacccggcgt tgccgaaggc gcttgcgttc tggttcacgg
     4081 ctgcgtgatc ggtggcggcg catcgaaaac gaatcgtcac cgtcggttcg ctcggctagg
     4141 gttggcgggt tcacgggggt gccaatgacg gacatgattc gcacggccgg actgacgaaa
     4201 cggttcgggg acgtaacggc gctggagggg ctggatctgg tcgttccgaa aggaaccgtg
     4261 ctgggcttgc tggggccgaa cggcgcaggc aagaccaccg cggttcggat cctgaccacg
     4321 ttgctggacc ccgacgaagg aacggtcgag atcgcgggcc tggacgcgat gcgcgagccc
     4381 gagaaggtcc gcgaaaagat cggtctgtcg ggccagtccg cggccgtgga cgagcacctg
     4441 tccggcttcg agaacctcga aatggttggc cgtctatacg gcctggaccg ccggcgcgcc
     4501 aaggtccgcg cctcgcagct gatggagcgc ttcgatctgg cgttcgccgg caagcgtatg
     4561 tccaagacgt actcgggcgg catgaagcgg cgcttggact tggcggccgc actcgtcgcc
     4621 gaacccgaag tgctcttcct cgacgagccc acgaccggcc tggatccgcg cagccgcctg
     4681 gcgctgtggg acgagatccg taccctggtt cgcggcggct cgacgctgct gctgaccacg
     4741 cagtacctgg aagaggccga ccgtttggcc gacgacatcg tcgtcatcga ccgcggccgc
     4801 atgatcgcgc acggcaccgc cgacgagctg aaagcgaaag tcggcggcga acggatcgag
     4861 atggtgctcg cctcgcccga ggacgaaggc gtcgtccgcc gcatcctcga gaagctgtcc
     4921 gacgaggaag tccagtacga ccccgatgcg cggcgattca ccgccgcggt gcccgacgga
     4981 caagaagcgc ttcgcaccgc gttgacggcg ttgaagcgat ccaagaccga ggtgcgcgac
     5041 gtcggattcc ggcggcccac gatggacgat gtcttcctga ccctgaccgg gcatcacgtg
     5101 gacggcgacg cccccgtcac cgagcaggaa gcggc
//
LOCUS       DATLFO010000181         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_122641, whole genome shotgun sequence.
ACCESSION   DATLFO010000181 DATLFO010000000
VERSION     DATLFO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122641"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1386)
                     /locus_tag="VM841_07225"
     CDS             complement(130..1386)
                     /locus_tag="VM841_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90008.1"
                     /translation="MNDESTGRLLLALTRDVTGTLDLQQVLDRSLAALRRLVPFGGGA
                     IQLIHNGALVAAATDPPMAPQARTVRIPVGSGVSGRIAARGEPVYIPDITIDARVHPE
                     GRAKGVSGGVRSYFGLPLIAHGEIIGVVQVDAPRVDAFSQRARETITAFVPAIAAAVQ
                     NATIHAHEIATVEERIETERLKHDFIAIVSHELRTPLTTVVGFAESLERLSRELDPEM
                     VSYLAGRILAGGRRLGRHLDDLMRVAGLLDGDLAVAIATVDVAPVVRAACAESTDPHH
                     PLRLRIEPSLPQVATDPDRLHQIVGALVSNACKFSPPGAEVTVRAGKDPDGSVRVSVQ
                     DRGRGISAEHIEQVFDRFFQVSPADRRETGGMGIGLYLARLLCDRMNARIDVRSAVGE
                     GSTFTVALPILVAPRDPTSAAAAGAS"
     gene            complement(1469..2197)
                     /locus_tag="VM841_07230"
     CDS             complement(1469..2197)
                     /locus_tag="VM841_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HVL90009.1"
                     /translation="MLDSETLDYFAGEAYITRPIRPMKSGKEATVVLCEAHPRTGRDL
                     IAIKEYKPIEERGFRADLAYMAADVGTLKGRDRRAIAKKTAWGHQMQEGLWIHREWER
                     LNELHAAGCTVPEPIAQGPRSIAMEYIGSHEAAAPQLHRVRLGHRQARSAWDAIVADV
                     ERMLAANVIHGDLSPYNILYWAGRPVIIDFPQCVDPRTARDGHALLERDLTNVARHFE
                     RSGLQLDPQSVSHGLWIAWTFADL"
     gene            complement(2379..>3129)
                     /locus_tag="VM841_07235"
     CDS             complement(2379..>3129)
                     /locus_tag="VM841_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90010.1"
                     /translation="AATFAIDGLTFLFALAMIRRMNPSPPHEAAPAPGFKSILEGFSF
                     LRGRPTLTGSFIIDLNAMIFGMPKALFPAMAALMGGGPKTVGLLYAAPSAGSFLAMAA
                     SGWTGKVRRQGLAVTIAVLGWGLSIAAFGLTRSVPVAVLCLAAAGFSDAVSGIFRTAI
                     VQEVTPDHLRGRLSGMEIAVYAGGPTLGDVEAGVVAGLTSVRFSVVSGGLACLVGAGL
                     TALMLPQFIRYDRTRAAAESEKPVDEPLPAT"
BASE COUNT          500 a         1076 c         1068 g          485 t
ORIGIN      
        1 gccaaaaggg cgccgacgca cagcgccacc atgagcattc gcagtcgcac aggtcccccg
       61 caaaatcgtc cgactcgttc gctttcttcg cagtcgccgg atttccttcc cgctcgatgc
      121 gatttccaat caggaggcgc cggccgcggc ggcgctcgtc gggtcccggg gggccaccag
      181 gatcggcagc gcgacggtga acgtgctgcc ttcgcccacg gcgctgcgca cgtcgatgcg
      241 ggcattcatc cgatcgcaca gcaggcgcgc cagatacagg ccgatgccca tgcctcccgt
      301 ctcgcgccgg tcggcgggtg agacttgaaa gaagcgatcg aagacctgct cgatgtgctc
      361 ggcggagatt ccgcgtccgc ggtcttgcac ggagacccgg acgcttccgt cggggtcctt
      421 gcccgcgcgc acggtcacct cggcgccggg cggcgagaac ttgcacgcgt tcgagaccag
      481 cgcgccgacg atctgatgca gacggtccgg atcggtggcg acctgtggca ggctcggctc
      541 gatccgcagc cgcaaaggat ggtgaggatc ggttgattcg gcgcaggcgg cgcgcacgac
      601 gggtgcgacg tccacggttg cgatcgcaac ggccaggtcg ccgtccaaca acccggcaac
      661 gcgcatcagg tcgtccagat gccggcccag ccgtcgtcct cccgccagga tccggccggc
      721 caggtacgac accatttcgg gatccaactc gcggctgagg cgctccagcg actcggcgaa
      781 tccgacgacc gtcgtcagcg gcgtgcgaag ctcgtgggaa acgatggcga tgaaatcgtg
      841 tttcagccgt tccgtctcga tccgttcctc gacggtcgca atctcgtgtg cgtgaatggt
      901 cgcgttttgc accgccgccg cgattgccgg cacgaacgcc gtgatcgttt cacgggcgcg
      961 ctgcgagaac gcgtccacgc gaggcgcatc cacctgcacg accccgatga tctcgccgtg
     1021 tgcgatgagc ggcaggccga agtagcttcg gacgccgccg ctgacgcctt tggcgcggcc
     1081 ctcgggatgc acgcgcgcat cgatggttat gtccgggatg tagaccggtt cgccgcgtgc
     1141 cgcgattcgg cccgacacgc cgctgcccac gggaattcga accgtccgcg cttggggcgc
     1201 catcggcgga tccgtcgcgg ccgcgaccag cgcgccgttg tggatcagct gaatcgcgcc
     1261 gccgccgaag ggcaccaggc gccgcagggc ggcgaggctg cggtccagca cctgttgcag
     1321 gtcgagcgtg ccggtgacgt cgcgggtcag cgcgagcagc agccggccgg tcgattcgtc
     1381 attcacgcac tgtcaccatg ccgatctccg gcgggctcgt ccatgccccg gcggggctag
     1441 tcgccgcggg caaatcgccc gcggccgctc aaaggtcggc gaaggtccag gcgatccaca
     1501 gaccgtggga aaccgattgc ggatccagct gcaggcccga gcgttcgaag tggcgcgcaa
     1561 cgttggtcaa gtcccgttcc agcagagcgt gcccgtcgcg cgccgtgcgg ggatcgacgc
     1621 actgcggaaa gtcgatgatc accggacggc cggcccagta caggatgttg tagggcgaca
     1681 ggtccccgtg gatcacgttc gcggccagca tccgctcgac gtcggcgacg atcgcgtccc
     1741 acgcgctgcg cgcttgccga tggccgagcc ggacgcggtg cagctgcggg gcggccgcct
     1801 cgtgcgagcc gatgtattcc atcgcgatcg aacgcgggcc ttgcgcgatg ggctcgggca
     1861 cggtgcatcc cgccgcgtgc aactcgttga gccgctccca ctcgcggtgg atccacaggc
     1921 cttcctgcat ctgatggccc catgccgtct ttttcgcgat cgcgcggcgg tcgcggccct
     1981 tgagcgtgcc gacgtcggcg gccatgtagg cgagatccgc gcggaagccg cgctcctcga
     2041 tgggcttgta ctccttgatc gcgatcaggt cgcggcccgt gcggggatgc gcctcgcaca
     2101 gcacgaccgt cgcctctttg ccgcttttca tgggccggat cggacgcgtg atgtacgcct
     2161 caccggcgaa gtagtccaga gtctcggaat cgagcacgat cggttctcct tgtgagatcg
     2221 aagacgacga agaacagaag tgcgccttcg aaggggaacc gcgtgagggg agctagcggg
     2281 cggtgaccgg aaggcgcgcg gcaactgcgg caatggccgt cataggtggc gcctccttcc
     2341 ggttcgggcc ggcagggctg ccggcaactc cgtcgatcct acgtcgccgg caggggctcg
     2401 tccacaggtt tttcggattc ggccgccgcg cgggtgcggt cgtagcgaat gaactgcggc
     2461 agcatcaatg cggtcaggcc cgccccgacc aggcacgcca gcccacccga gaccacgctg
     2521 aagcgcaccg acgtcagtcc ggcgaccacg cccgcctcga cgtcgcccag cgtcggtccg
     2581 cccgcgtaga ccgcgatctc catgcccgac aggcgtccgc gcaagtgatc gggcgtgacc
     2641 tcttgcacga tcgcggtgcg gaagatgccg ctcacggcgt cgctgaaccc ggcggcggcg
     2701 agacacagca ccgcgaccgg aacgcttcgc gtcagtccga acgcggcgat cgacagaccc
     2761 catccgagga ccgcgatggt gacggccaag ccttgccggc gcactttgcc ggtccatccg
     2821 ctcgcggcca tcgccaggaa cgagccggca ctcggtgccg cgtacagcaa gccgaccgtc
     2881 ttgggcccac cgcccatcag cgccgccatc gcggggaaca gcgccttggg catgccgaaa
     2941 atcatcgcgt tcaggtcgat gatgaacgag cccgtcagcg tcggcctgcc gcgcaggaac
     3001 gagaaccctt ccaggatcga cttgaagccg ggtgcgggcg cggcctcgtg cgggggcgac
     3061 gggttcatgc ggcggatcat cgccagcgcg aacagaaagg tcagcccgtc gatcgcgaac
     3121 gtggcggcc
//
LOCUS       DATLFO010000182         5603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_106051, whole genome shotgun sequence.
ACCESSION   DATLFO010000182 DATLFO010000000
VERSION     DATLFO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106051"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VM841_07240"
     CDS             complement(<1..512)
                     /locus_tag="VM841_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90011.1"
                     /translation="MSVPIRPAPNPRWEPRPEEPSSLEIQDAVLEADDEDSYDEDVAK
                     ILTEIQSVTAVAELEARVAELEHELSRIRSGHLDEADMVTGELTIMKARIEDALGAVA
                     STVTELRAEAAKAAELPEPVAPVIDEAALRDRVRAEIRPARDELVATTREEVNTRVAQ
                     MRIDLDASINT"
     gene            complement(611..1783)
                     /gene="thiI"
                     /locus_tag="VM841_07245"
     CDS             complement(611..1783)
                     /gene="thiI"
                     /locus_tag="VM841_07245"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HVL90012.1"
                     /translation="MRDYAILHYHEIGLKGGNRSFFEKALARNVDRTLKGLAGNTKRM
                     PGRLLVPLTAGADTGEVIARLRRVYGIANFGLATGAEMTLEQIYEVAWDHMRRTDAWS
                     TFAVRARIAHSSLPYTVRELNEKIGAVLLERSGAKVNLSAPDLTVHVEIVGDLVLVYA
                     DKQQGAGGLPVGVSGRSVVLISAGIDSPVAAARMMRRGSKVSFVHFHSMPYTDGSSPR
                     QAEEIVTLLTRHQNDANLWMVPLAPSQQQIVAVAPQDLRTILYRRMMMRIAALVAGQE
                     GAKALVTGDSLGQVASQTLENLTTVEDASPLPVLRPLIALDKIEIIDEAHRLGTFEAS
                     SAPCQEACVLFEPKRPATKASVQEARRAEAKLDVDALAREAFANAELRQYRYPEPA"
     gene            complement(1997..3181)
                     /locus_tag="VM841_07250"
     CDS             complement(1997..3181)
                     /locus_tag="VM841_07250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HVL90013.1"
                     /translation="MNWVDLVILGALGLGIWTGYRRGAVMQIFSWGGFVLGLVLGFLL
                     APPLVGTFVKDPESASIPIYTIVALLGIAFIVEGIVAAFAMRLRKRITNATVNKTDKI
                     IGSAIAALLSLVGSWLLGSTLRSVPAKSVASAVRGSAIIRVLNDIAPPPLSITAEISR
                     LLRNSGFPEVFAALNPSLAPGVEPPPAALADDREVLAAAEITYKIESRGCNGLVDGSG
                     FPIGSDLLVTAAHVVAGTSDHKVIPATGRRTFQATVVYMDTDKDIAIMRVKLPGKVFF
                     VESDHASRNTDGAAIGYPGGGKRKISPARVRSRTQARGYDIYSQGTVTRTIYVLHAEV
                     RQGNSGGPFVDEDGKVRGMVFAASREDPNESYALAETEIFRAVDRAKGKTKQVDTGRC
                     AL"
     gene            complement(3192..3701)
                     /locus_tag="VM841_07255"
     CDS             complement(3192..3701)
                     /locus_tag="VM841_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVL90014.1"
                     /translation="MGEARDFGRFYEDFEVGDVYKHWPGKTITEYDDHLFCMITMNHH
                     PLHTNVHYAETSTHFKRNVVVGNLVYSLALGMSVPDVSGRAIANLEVESLKHTAPMFH
                     GDTLYAETKVLDKKESSSKPDRGIVAVETRGYNQDGVEVLYFRRKVMVPKKSHSPETQ
                     PGRPEPKAE"
     gene            complement(3759..4415)
                     /locus_tag="VM841_07260"
     CDS             complement(3759..4415)
                     /locus_tag="VM841_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HVL90015.1"
                     /translation="MNEHVLEALQAESDRAADFLESLTAGDLGSPTRCPPLSVLEVAA
                     HAFRGSRRIVEMIEAGPVEAEAEKSAITYFRYDADAEASAIVQRAQEVARTFEPAAWR
                     PGWDEALRQAEPHLKVDPVFGTVFGLMHLSEYLKTRVVEVVIHAMDMRHALNLSPDPT
                     GPGLTACCDVLRGLLGADVRPLGMDDVRFAETATGRATLDDAEREMLGPLADLFPVLA
                     "
     gene            complement(4412..5590)
                     /locus_tag="VM841_07265"
     CDS             complement(4412..5590)
                     /locus_tag="VM841_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90016.1"
                     /translation="MALLNLTEEQKLIVDTVREFVEKEVIPVADEMEHKDEFPEKIVE
                     QMKEMGLFGMMVPEEYGGLGMPLTVYALVIVELARGWMSLSGILNTHSMACYLIKTYG
                     TDEQKQRFLPQMVTGNPRASLLMSEPHAGSDVQAIKTRAVRDGDDYVINGQKMWATNG
                     LRSGICMTITKTADTDPPYKGMSMFIVEKEPGAERSGGVIVGKNIEKMGYKGVETTEV
                     AFDDHRVPAANLLGGEEGQGFIQNMGAIEVGRVNVGARAVGVAQRAFEEAIAYAQERT
                     TFGKPIAQHQAIQFYLAEMATKIEAARLLMLNAAIMKDEGLRTDLEAGMCKLYASEMA
                     MEVVRDAVRVHGGYGYSKEYTVERLYRDAPFFVIGEGTSEIQKLVIAKNLLDRYKVKK
                     "
BASE COUNT          828 a         1852 c         1917 g         1006 t
ORIGIN      
        1 gtgttgatcg aggcgtccag gtcgatgcgc atctgcgcga cgcgcgtgtt gacctcttcg
       61 cgcgtggtcg cgacgagctc gtcgcgcgcg ggacggatct cggcccggac ccggtcccgc
      121 agggcggcct catcgatgac gggggcgacc ggctcgggga gctcggctgc cttggcggcc
      181 tcggcgcgga gctcggtcac ggtcgaggcg accgcgccca gcgcgtcctc gatccgggcc
      241 ttcatgatcg tgagctcgcc ggtgaccatg tcggcctcat cgagatggcc actgcggatc
      301 cggctcagct cgtgctcgag ctcggccacg cgggcctcga gctcggcgac ggcggtgacg
      361 gactggatct cggtgaggat cttggcaacg tcctcgtcgt aggagtcctc gtcgtcggct
      421 tcgaggacgg cgtcctggat ctcgagcgac gaaggctcct ccggccgggg ctcccagcgg
      481 ggattcgggg caggcctgat cgggacgctc atgtctgatc ctccagggct gaccctagcc
      541 gtgccccacc cggagtcaag catgcccggc gactgcgcgg gctcgatcgg agggcggaac
      601 tggtcgtcgt tcatgcgggc tcaggataac gatattggcg aagctcggcg ttcgcgaaag
      661 cctcgcgggc gagggcgtcc acgtccagtt tcgcctcggc ccggcgggct tcctgcacgg
      721 aggccttggt cgcgggccgt ttgggctcga acagcacaca cgcttcctgg cagggagccg
      781 acgaggcctc aaaggtcccc agacggtggg cctcgtcaat gatctcgatc ttgtccaggg
      841 cgattagcgg ccgcagcacg ggaagcgggc ttgcgtcctc gacggtcgtg aggttctcca
      901 gcgtctgcga ggccacctgg ccgagcgagt cgccggtgac cagcgccttg gcgccttcct
      961 gcccggcgac cagcgcggcg atgcgcatca tcatgcgccg gtacaggatc gtgcgcaggt
     1021 cctgcggcgc gaccgcgacg atctgctgct ggctgggcgc cagcggcacc atccacagat
     1081 tcgcgtcgtt ctggtggcgg gtcagcagcg tgacgatctc ctccgcttgc cgggggctgg
     1141 agccgtcggt gtagggcatc gagtggaagt gcacgaagct gaccttgctc ccccgccgca
     1201 tcatgcgcgc ggccgcgacc ggcgagtcga tgccggcgct gatcagcacc acgctgcggc
     1261 cgctgacccc gacgggcagg ccgccggcgc cctgctgctt gtcggcgtag accaggacca
     1321 ggtcgccgac gatctcgacg tgcacggtca ggtcgggggc gctcaggttg accttggcgc
     1381 cgctgcgctc cagcagcacc gcgccgatct tttcgttgag ctcgcgcacg gtgtagggaa
     1441 ggctggagtg cgcgatgcgc gcgcggacgg cgaaggtgct ccaggcatcg gtgcggcgca
     1501 tgtggtccca ggcgacctcg tagatctgct cgagcgtcat ctcggcgccg gtcgcgaggc
     1561 cgaagttcgc gatgccgtag acgcggcgga gacgcgcgat cacctcgccg gtgtcggcgc
     1621 ccgcggtcag cggaacgagc aaacggccgg gcatccgctt ggtattcccg gccaagccct
     1681 tcagcgtccg gtcgacattg cgggccagag ccttttcgaa gaacgaccgg ttgccgcctt
     1741 tcagaccgat ctcgtggtag tggaggatcg cgtagtcacg cacgggctca tggtaccggc
     1801 cgggcacgtc cgagtttctc tggacactga agtacctatg cggttctcgg atgaccaaag
     1861 aaacgccgtc ccgcggatca ggccggctcg gcgagcatcg aacggatcgt gccgtggtca
     1921 ccggtggctg agtggggacg gcccggcgtt tcctcgacac gcgccccgca cccgacgcag
     1981 ccgatcactg cggctcctac agggcgcagc ggccggtgtc gacctgtttg gtcttgcctt
     2041 tggcgcggtc cacagcgcgg aagatctcgg tctcggcgag tgcgtaggac tcgttgggat
     2101 cctcacgcga ggccgcgaag accatgccgc gaaccttgcc gtcctcgtcc acgaacggcc
     2161 cgccggagtt gccttggcga acctcggcgt gcagcacgta gatggtgcgg gtgaccgttc
     2221 cttgcgagta gatgtcgtag cctcgcgcct gggtgcggct gcggactctt gcgggcgaga
     2281 tcttgcgctt gccgccgccg gggtagccga tcgcggcgcc gtcggtgttg cgcgaggcgt
     2341 ggtcgctctc gacgaagaac accttgccgg gcagcttcac ccgcatgatc gcgatgtcct
     2401 tgtcggtgtc catgtagacg accgtcgcct ggaaggtccg gcgtcccgtg gcgggaatca
     2461 ccttgtgatc ggaggtgccg gcgaccacgt gcgccgcggt gaccagcagg tccgacccga
     2521 tcgggaaccc cgagccgtcc accagcccgt tgcagccccg agactcgatc ttgtaggtga
     2581 tctcggcggc ggccaggacc tctcgatcgt cggccagcgc ggcgggcggc ggctcgacgc
     2641 ctggcgccag cgacgggttg agcgcggcga agacttcggg aaagccgctg ttgcgtagca
     2701 gccggctgat ctcggcagtg atcgacagcg gcggcggcgc gatgtcgttg agcacgcgga
     2761 tgatcgccga gccacgcacc gctgacgcga ccgacttggc cggcacgctg cgcagcgtcg
     2821 atccgagcag ccaggatccg accagggaca gcagcgccgc gatcgcagag ccgatgatct
     2881 tgtcggtctt gttgacggtc gcgttggtga tccgcttgcg gagccgcatc gcgaacgcgg
     2941 cgacgatgcc ctcgacgatg aacgcgatcc cgagcagcgc cacgatcgta tagatcggga
     3001 tcgaggcgct ctcgggatcc ttgacgaatg tgccgaccag cggcggggcc agcaagaagc
     3061 cgagcaccaa gccgagcacg aacccgcccc aggagaagat ctgcatgacg gcgccccgcc
     3121 ggtagccggt ccagatgccc aggccgagcg cgccgagaat gacgagatcg acccagttca
     3181 accagaccct cctattcggc cttcggctcg gggcgtccgg gctgcgtctc ggggctgtgg
     3241 gacttcttcg gcaccatgac cttgcggcgg aagtacagca cctcgacgcc gtcctggttg
     3301 tagccgcggg tctcgaccgc gacgatgccc cgatccggct tggaggagga ttccttcttg
     3361 tcgagcacct tcgtctcggc gtagagcgtg tcgccgtgga acatcggcgc ggtgtgcttg
     3421 agcgactcga cctcgaggtt ggcgatcgcg cggcccgaca catccggcac gctcatgccc
     3481 agcgccagcg agtacaccag gttcccgacg actacgttgc gcttgaagtg ggtcgaggtc
     3541 tcggcgtagt ggacgttggt gtgcagcggg tggtggttca tcgtgatcat gcagaacaga
     3601 tggtcgtcgt actcggtgat cgtcttgccc ggccagtgct tgtagacgtc gccgacctcg
     3661 aagtcctcgt agaagcgtcc gaagtcccgt gcctcaccca tcgtggcgtt cctcctcgca
     3721 gtgtcggaag tgtactttcg gatcggagtc ctcgctggct acgcgaggac gggaaacaga
     3781 tcggccagcg gtccgagcat ctcgcgctcg gcgtcgtcga gcgtcgcgcg gccggtcgcg
     3841 gtttcggcga agcgcacgtc gtccatgccg agcggccgca cgtccgcgcc cagcagcccg
     3901 cgcagcacgt cgcagcacgc ggtcagcccc ggcccggtcg ggtccggcga gaggttgagc
     3961 gcgtgccgca tgtccatggc atggatcacg acctcgacca cgcgagtctt gaggtactcg
     4021 gacaggtgca tcaggccgaa cacggtgccg aagaccggat cgactttcag gtgcggctcg
     4081 gcctgcctca gcgcctcgtc ccagccgggc cgccatgcgg cgggctcgaa cgtcctggcg
     4141 acctcttgcg cgcgctgcac gatcgccgac gcttcggcgt cggcgtcgta gcggaagtag
     4201 gtgatcgcgc tcttctcggc ctcggcctcg acggggccgg cctcgatcat ctcgacgatg
     4261 cggcgcgagc ctcggaaggc gtgcgcggcg acctcgagca ccgacagcgg cgggcaccgc
     4321 gtcggcgacc ccagatcacc tgccgtgagc gactccaaga agtcggcggc ccgatcgctc
     4381 tcggcctgga gggcttcgag cacgtgctcg ttcacttctt gaccttgtag cggtcgagca
     4441 gattcttggc gatgacgagc ttttggatct cgctggtgcc ttcgccgatg acgaagaacg
     4501 gcgcgtcgcg atacagccgc tcgacggtgt actccttcga gtagccgtag ccaccgtgca
     4561 cacgcaccgc gtcgcgcacg acctccatgg ccatctcgga ggcgtagagc ttgcacatgc
     4621 cggcctcgag gtcggttcgc agaccttcgt ccttcatgat cgccgcgttg agcatcagca
     4681 gccgcgcggc ctcgatcttg gtggccatct cggccaggta gaactggatc gcctggtgct
     4741 gcgcgatcgg cttgccgaag gtcgtgcgct cctgcgcgta ggcgatcgcc tcttcgaagg
     4801 cgcgctgcgc gacgccgacg gcgcgtgcgc cgacgttgac gcggccgact tcgatcgcgc
     4861 ccatgttctg gatgaagccc tggccttctt cgccgccgag caggttcgcg gccggcactc
     4921 ggtgatcgtc gaaggcgacc tcggtggtct caacgccctt gtagcccatc ttttcgatgt
     4981 tcttgcccac gatcacgccg ccgctgcgct cggcgccggg ctctttctcg acgatgaaca
     5041 tcgacatgcc cttgtagggc ggatcggtgt cggcggtctt ggtgatcgtc atgcagatgc
     5101 cggagcgcag gccgttggtc gcccacatct tctggccgtt gatcacgtag tcgtcgccgt
     5161 cgcgcaccgc gcgcgtcttg atcgcctgga cgtcgctgcc ggcgtgcggc tcgctcatca
     5221 gcagcgaggc gcgcgggttg ccggtgacca tctgcggcag gaagcgctgc ttctgctcgt
     5281 cggttccgta ggtcttgatc agatagcacg ccatcgagtg cgtgttgagg atccccgaca
     5341 gcgacatcca gccgcgcgcc agctcgacga tgacgagcgc gtagacggtc agcggcatgc
     5401 ccagcccgcc gtactcttcg ggaaccatca tcccgaacag gcccatctct ttcatctgct
     5461 cgacgatttt ttcggggaac tcgtccttgt gctccatctc gtcggcaacg gggatgactt
     5521 ccttctcgac gaactcccga acggtatcga cgatcagctt ctgttcttcg gtcagattca
     5581 gcagtgccat cgcgatcgct cct
//
LOCUS       DATLFO010000183        11730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_133102, whole genome shotgun sequence.
ACCESSION   DATLFO010000183 DATLFO010000000
VERSION     DATLFO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133102"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VM841_07270"
     CDS             <1..983
                     /locus_tag="VM841_07270"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVL90017.1"
                     /translation="MGILSGGSRFAVERGFGTERDLEYCEDGDAVGDADPSQVGERAR
                     QRGLMQVGSLGSGNHFLEIQYVERVDDAERARAFGLDEGRVAVMIHCGSRGLGHQVCT
                     DHVRSMQGAMARYGIEVPDPQLACVPVDSKEGRSYMGAMAAAANYGRANRHLLAEAAR
                     DAFAKATGVRALELLYDVSHNLAKIESHGGRTLCVHRKGATRALPPGHAELPAAFAET
                     GQPVLIPGSMGTASYVLAGVEGGDAFHSTCHGAGRAMSRHAAKRSIQGGALRRELERA
                     GIRVRVRSDQELAEEAPSAYKDVDAVVATCERAGLCRRVARLRPLGVIKG"
     gene            complement(980..1402)
                     /locus_tag="VM841_07275"
     CDS             complement(980..1402)
                     /locus_tag="VM841_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HVL90018.1"
                     /translation="MNRGEAGHRILPHTADLLIEAWGPSRARCFEEAVRALVSTFVDV
                     VEVPASEPVSISIDAGADNEMLAAVLDEILFLADVSGRVPIHLTLEESEDGGLAGVLD
                     VIAIDGLDVVGPLPKGASYGGEVAESGGLWHCRVVVDI"
     gene            complement(1483..2439)
                     /locus_tag="VM841_07280"
     CDS             complement(1483..2439)
                     /locus_tag="VM841_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009487372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HVL90019.1"
                     /translation="MKIGYALSSEEHAPSDLVADAQRAEAAGFDFLMISDHFHPWVDR
                     QGHSPFVWNVLGGIAATTERIPIGTGVTCPIIRIHPAIVAQAAATTAAMMPGRFWLGL
                     GTGENLNEHVLGDPWPPGDVRLEMLEEAVEVIRELWTGEAVSHRGRYYTVQQARIYTL
                     PDELPPILIAAKGERATRLAAGSDGLISTVPEDEVVQNFENAGGAGKPKIGMMHVCWA
                     RDEADVRKTAREWWPNTSVPGELTVELPLPRHFEQASQNIDEDDVASSITVGPALGGY
                     VEAVRKYTDAGYDHVYIHQIGPDQSGFIDFARAELLPAIRSS"
     gene            complement(2481..2717)
                     /locus_tag="VM841_07285"
     CDS             complement(2481..2717)
                     /locus_tag="VM841_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90020.1"
                     /translation="MKIRTLLMAAAAGAAVAYFMDSERGAQRRAVAKQRAEQFKAQAL
                     PKIQELRRYTAADMPGDEEASYASNGSSTSALPS"
     gene            complement(2789..3724)
                     /locus_tag="VM841_07290"
     CDS             complement(2789..3724)
                     /locus_tag="VM841_07290"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HVL90021.1"
                     /translation="MSVASAERVPGVVVLTPRQTRLHQFALLSFSIVPLIGVGLAIWS
                     LWGRGLSGTDAAIFGGMYASTGLGVSAGFHRLFAHKTYRAKAPARIVLAVAGSMSIEM
                     GVIKWAATHRRHHAFADRPGDPHSPHLDAAQGLKGIARGLWFAHMGWLLTSERTDPQR
                     WAPDLLKDPALRWIDRAFPLWVALSVLIPPALGLAITGSWAGAATAFLWGSLVRIFLL
                     HHVTFSINSICHFFGKRPYDTKEESRNVWPLAFISFGEAWHNNHHAFPASSSLGLRWW
                     QVDPGTILIRGLRLIGLADDLRRPSVREIRQARAG"
     gene            3960..6311
                     /locus_tag="VM841_07295"
     CDS             3960..6311
                     /locus_tag="VM841_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL90022.1"
                     /translation="MRSKRRTGLRTRAIALVLAGAAPALLALGIRAVQEHNAAGDAAR
                     RDALGVSRAIAAEQSTLIDAARVMLAGVAELPQIAAGGPQCASLLEALRKANPAYANL
                     GVAGPDGAVTCSAFAMPAGTRIEDRPYFRDAVATGGFSIGEYQIGRITGTASLNVGHP
                     IVAGGELRGVVFAAIRLTALNDIARRTVLPPGSVVTILDRNGTILARAPDPEGYVGKR
                     FEDSRLAAALARRSGSTLEAHGFDGVKRLYGHVRTGGSDGNEITVAVGIPSAAAFANA
                     NRARNAALAGLAIVTVLSVMAGMIGSRRLVRPVRRMAETARRIEAGDMQARSDLPATG
                     ELGELARSFDDMAVSLERRLSALRYLTHHDTLTELPNRRALEDAIRTGESTGPGALLL
                     ININRFRDVNHTLGPRSGDLVLREAADRMRSLMESGVMVARVGGDEFAILLEGVDRRE
                     ATIAAMGVVDQFETPCMLGDIEVDMRTRVGVAPFERPADPNEVIRRAEVAERTCRDRG
                     LICLVYSERQDPYTDQRLRLMGALRGAESRGQMHLVFQPMVDTVTSRPVRVEALLRWR
                     HPELGEVPPDLFIPLAEDFGLIRRLTTWVLGEAVREHKRWASEGIFLPVAVNISAISL
                     TDPELTATIEQLLRREGVPPEAIELEITETAAMAQPEEALRTLTHLSSRGIRIAIDDF
                     GTGHSSLSYLSRLPIDTVKIDRSFIAALDGDDDAGILVRSTIELAHKLRRSVVAEGVE
                     DETTHRILQAAGCDCIQGFLVGTPMTSDRLVEWARGVAPAMAG"
     gene            6411..6845
                     /locus_tag="VM841_07300"
     CDS             6411..6845
                     /locus_tag="VM841_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90023.1"
                     /translation="MTSPRAETTAQTPSLEHAVAALEALVGAKPHPDAVPKALRTVRQ
                     ALEDHCATMEEPQGGFDQILSHAPHLSHEVAGLRIEHAAARASLDNLVEMIDHAPSLH
                     HPAMLVAMIGRVRLLIAAIERHHRHTAHTLHEAFVAETGEID"
     gene            complement(6842..7903)
                     /locus_tag="VM841_07305"
     CDS             complement(6842..7903)
                     /locus_tag="VM841_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HVL90024.1"
                     /translation="MTPHIAPEDALAVDPAGIARYLAARLPQYGGEFGVTRLGEGQSC
                     LTFMLEGEGWRVVLRRPPRGDLPPSAFDVTREYRVMSALHSHDAPVPVPPPVALCTDT
                     GVIGAPFYLMEPVNGLVVRTELPEALSSMDDRARMARALVDTLVALQSIDYRAAGLEG
                     FGKPEGYLERQLRRMNQLWELARFRDLPDIEAVGKWLSENIPSQRRATIVHGDYKLDN
                     VIFAPESPARLVAVVDWEMSTIGDPLADLGWMLYWWRDPGDPSFGLTVSSVMDQEGFP
                     RRRELLQAYAGATGEDVSAIDWYAALGGWKIAIIMEGSYRRFQAGITDHPLFKELEWG
                     VPALAARSAAAMRGELGLR"
     gene            complement(7900..9123)
                     /locus_tag="VM841_07310"
     CDS             complement(7900..9123)
                     /locus_tag="VM841_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90025.1"
                     /translation="MDFSFGPKVEELRKNLLEFMDSRVYPAERIWHEQVASLPDPHHS
                     PPVMDDLKKEARARGLWNLFMPDPAYGPGLSVSEYAPLAEITGRVPHIAPEAINCAAP
                     DTGNMEIIAEFGTDEQKKTWLEPLLNGEIRSAFAMTEPDVASSDARNISARIERDGDT
                     YVINGRKWWTSGAADPRCKILIFMGVTNPDADSYARQSMVLVPIDTPGVHVIRTLPVF
                     GYDEGHGHCEIEFTDVRVPVANLLGEEGGGFRIAQSRLGPGRIHHCMRAIGMAERAFD
                     LMCRRALTREAFGKRLAQQGVVQNQIAESRIEIEQARLLVLKAAWLIDTHGKEAARVE
                     ISAIKVAVPRMALAVIDRAIQIHGGMGVSDDVPLAAMYAGARTLRIADGPDDVHLMVL
                     ARRELARYIPEEMKT"
     gene            complement(9163..10404)
                     /locus_tag="VM841_07315"
     CDS             complement(9163..10404)
                     /locus_tag="VM841_07315"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90026.1"
                     /translation="MKPGMHTRGPLIALLSSSGVSMAGNVIAGIAVPWFVLRTTGSAT
                     RAGFIAAMSFLPTVVASLFGGVLIDRLGFKRTSVLADVLSGVTVAAIPLLHATVGIEF
                     WQLAMLVFTGSLLDAPGVIAREALVPRLAEDAGMSIERGTASIQVVERSSRLAGAPLA
                     GLIIAAAGAPAALWINAASFLVSAVAVAVAVPGSSLRSDGRGRYWAEMREGLGFVWRD
                     PVIRWIGITVMVTNLLDAPLYAVVLPVLAERIYDSPVVLGLMLGAAGGGAVVGALLFG
                     WRGSTIPRRRVFVGGFATLAAFLWVLIAFPPVPVLVAVMAVKGVAAGPLNPVIMAIQF
                     ERVPERLRGRVFGATLALSWASIPAGTITAGWLIERAGIRTVMIAIAVAYLLWTAGMA
                     ANPVFRRLDESRRIPASEGSL"
     gene            10519..11004
                     /locus_tag="VM841_07320"
     CDS             10519..11004
                     /locus_tag="VM841_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HVL90027.1"
                     /translation="MSKVTEYLQQHHVPFTTLGHAAAASAVAEAYVLVLPPWKVAKTV
                     VLDTSKGHVIAVVPADRRVDLPRVRAALGDPHATLADEAEIARDYPEFELGAIPPIGR
                     LTGTTTLVDPEIAGLETIVFASGRQDESVRIATGDLLDDANVRVLPIVVAPTFEDDWL
                     Q"
     gene            complement(11057..11446)
                     /locus_tag="VM841_07325"
     CDS             complement(11057..11446)
                     /locus_tag="VM841_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL90028.1"
                     /translation="MKIKQITLEKAVTADVEESLADAADRMRFFDVGALPVTEGGRLV
                     GILTERDIVRAVANGARTELTPAGDYMTPSPATIGPEADLEHALGIMVALRVRHLPVV
                     DGGALTGIVSLRDLLGAERDLSGVHPV"
     gene            11610..>11730
                     /locus_tag="VM841_07330"
     CDS             11610..>11730
                     /locus_tag="VM841_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL90029.1"
                     /translation="MTDDVVTVRPDTSFREIVSLLMERRVSGVPVVDETGTVVG"
BASE COUNT         1968 a         4030 c         3928 g         1804 t
ORIGIN      
        1 tgatgggcat cctctcgggg ggctcgcgct tcgccgtgga gcgcggtttc gggaccgaac
       61 gcgacctcga gtactgcgag gacggcgacg cggtcgggga cgccgaccca tcgcaggtcg
      121 gcgagcgagc gcggcagcgc ggcctgatgc aggtcggcag cctcgggtcc ggcaatcatt
      181 ttcttgagat ccaatacgtc gagcgcgtgg atgacgcgga acgtgcgcga gcgttcggtc
      241 tcgacgaggg acgagtcgcg gtgatgatcc attgcggatc gcgaggcctc ggccatcaag
      301 tctgcaccga tcacgtacgc tctatgcaag gcgcgatggc tcgatacggc atcgaggtcc
      361 ccgatccaca gctggcctgc gttccggtgg actcgaagga gggccgctcg tatatgggtg
      421 cgatggccgc ggccgccaac tacggccggg cgaaccggca tctgctcgcc gaggctgctc
      481 gcgacgcatt cgcgaaggcg accggcgttc gcgcactgga actgctctac gacgtgtccc
      541 acaacctggc caagatcgag tcgcacggcg gccgcaccct ctgcgttcac cgcaaaggcg
      601 cgacgcgcgc gctcccgccg ggacacgctg agctgcctgc ggcattcgcc gagacgggac
      661 agccggtgct gatcccgggc tcgatgggga cggcgtccta cgtgctcgcc ggcgtcgagg
      721 gaggcgacgc gtttcattcg acgtgtcacg gagcgggacg cgccatgagc cgccatgccg
      781 cgaagcgcag catccaggga ggcgcgctgc ggcgtgaact cgaacgagca ggcatccggg
      841 ttcgcgtgcg atccgatcag gaacttgcgg aagaggctcc ctcggcgtac aaggacgtgg
      901 acgcggtggt ggcgacctgc gagcgcgccg ggctgtgccg gcgagtggcg cgactgcgtc
      961 cgctcggcgt gatcaagggc tagatgtcca ccacgacgcg gcaatgccac aacccgccgg
     1021 actccgccac ctctcccccg taggacgcgc ccttgggcaa cggtccgacg acatcgaggc
     1081 cgtcaatcgc tatgacgtcg agcacgccgg cgagcccgcc gtcctcggac tcctcgagcg
     1141 tcaggtgaat gggcactcgt ccggaaacat cggctaggaa caggatctcg tcgagcaccg
     1201 cggccagcat ctcgttgtcg gcacctgcgt cgatcgagat cgagaccggc tcgctcgcgg
     1261 gaacctcgac aacgtcgacg aaggtggaca cgagcgcgcg caccgcttcc tcgaagcagc
     1321 gagcccggct cggtccccag gcctcgatca gcagatccgc cgtgtgcggc aggattcggt
     1381 gtccggcttc gccacgattc acacgactac ggtactcctc cgcctggacc gatcccgcgc
     1441 ccaccgggcc cgtcgtggac ggcggagcgc tgaccaccta catcaggagc tgcgaatcgc
     1501 gggaagcaac tcggcgcggg cgaagtcgat gaacccgctc tgatccgggc cgatctggtg
     1561 aatgtagacg tggtcgtagc ccgcgtcggt gtacttccgc accgcttcga cgtagccgcc
     1621 gagggcggga ccgaccgtga tcgaggacgc cacgtcgtcc tcgtcgatgt tctgcgaggc
     1681 ctgctcgaag tggcggggca acggcagctc gaccgtcagc tcaccgggca ccgacgtgtt
     1741 cggccaccat tcccgggccg tcttgcgcac gtcggcctcg tcgcgggccc agcagacgtg
     1801 catcatgccg atcttgggct tgcccgcgcc gccggcgttt tcgaaattct gaacgacctc
     1861 gtcttccggg accgtgctga tcagtccgtc gctcccggcc gcaaggcgcg tcgctcgttc
     1921 tcccttcgcg gcgatcaaga tgggtggcag ctcgtcgggc agcgtgtaga tgcgcgcctg
     1981 ctggaccgtg taataccggc cgcgatgcga gacggcttcg ccggtccaga gctcgcggat
     2041 cacctcgacc gcttcctcga gcatctccag ccggacatcg cccggcggcc acgggtcccc
     2101 gagcacgtgc tcgttgaggt tctccccggt gccgaggcct aaccagaagc ggcccggcat
     2161 catggcggcc gtcgtcgccg ccgcttgcgc gacgatggcg ggatggatgc ggatgatcgg
     2221 gcacgtgaca ccggttccga tcgggatccg ctcggtcgtg gccgcgatgc cgcccaggac
     2281 attccacacg aacgggctgt gcccctggcg gtccacccat gggtggaaat gatccgagat
     2341 catcaaaaag tcgaacccgg ccgcctccgc gcgttgcgcg tctgcgacca ggtccgacgg
     2401 tgcgtgctct tcgctcgaaa gcgcgtatcc gatcttcatg ctcctgagat acccgcgcga
     2461 gcaggtgcgc aatccgcccg ctacgagggc agggccgacg tgctgctgcc attcgaggcg
     2521 tacgaggcct cctcgtcacc gggcatgtcg gcggccgtgt acctgcgcag ctcctggatt
     2581 ttgggcagcg cctgggcttt gaactgctcg gcccgctgct tcgccaccgc gcggcgctgg
     2641 gcgccccgct cggagtccat gaagtaggcg acagcggctc cggccgcggc cgccatgagc
     2701 aacgtcctga tcttcatcgt cgatgctcct ttcacgctcg ccgggtccgg ccaatgaata
     2761 cccaatcgga gccgaaaacc aaccggcgtc agcctgctct ggcctgcctg atctcgcgga
     2821 cggacggccg acgcaggtcg tccgcgagcc cgatcagccg caacccgcga atcaggatgg
     2881 ttcccggatc gacctgccac caccggagcc ccagcgacga ggacgcggga aacgcgtgat
     2941 ggttgttgtg ccaggcttct ccgaacgaga tgaacgccag cggccaaacg ttgcggctct
     3001 cttccttcgt gtcgtaggga cgcttgccga agaagtgaca gatcgagttg atgctgaagg
     3061 tcacgtggtg cagcaggaag atccgaacga ggcttcccca cagaaacgcc gtcgcagccc
     3121 ccgcccatga ccccgtgatc gccaagccga gcgcgggcgg gatcagcacc gaaagcgcga
     3181 cccacagcgg gaacgcacgg tcgatccagc ggagcgcggg atccttgagc aggtcgggcg
     3241 cccagcgctg cggatcggtg cgctccgacg tgagcagcca ccccatgtgc gcgaaccaca
     3301 ggcctcgcgc gatacctttg aggccctgcg cggcatcgag gtgaggggag tgcggatcgc
     3361 cgggccggtc ggcgaacgcg tgatggcgcc ggtgcgtcgc ggcccacttg atcacgccca
     3421 tttcgatcga catcgagcct gcgaccgcga gcacgatacg cgccggcgcc ttcgcgcggt
     3481 aggtcttgtg cgcgaacagg cgatggaagc cggcgctgac tcccaaaccg gtggaggcgt
     3541 acatgccgcc gaagatcgcg gcatcggttc cgctgaggcc gcggccccac agggaccaga
     3601 tcgccaagcc gacaccgatc aaaggaacga tgctgaacga gagaagggcg aactgatgca
     3661 ggcgggtctg gcgtggcgtg agcacgacga ccccgggcac tcgctcggcc gaagcgacac
     3721 tcatgaaccc tccctgttcc gaaactttcg ccaccctaac attcgatttc ggacctagac
     3781 ttccccgaaa tcgcgggccg aatcaaccaa tttggcgggg ttcgccccct tccttgggtg
     3841 cgatacccag aaatcacgta ttccctttgt ggaaacctct ggctacgctg cgtgacgggc
     3901 cgcctcgatc catgcttgtt tccaccccgc ccggtggccc tcgtcgaccg aggattcccg
     3961 tgcgctcgaa acgacggacc ggccttcgca cccgtgcgat cgccctcgtt cttgccggag
     4021 cggctccggc tttgctggcg ttggggatca gggccgtcca ggagcacaac gccgccgggg
     4081 acgccgcccg cagggatgcg ttgggcgtct cgcgggccat cgcggccgag cagagcacat
     4141 tgatcgacgc cgcccgcgtc atgctcgcag gggtggccga gcttccccag atcgctgccg
     4201 gcgggccgca atgtgcctcg ctgctcgaag cgcttcgcaa ggcgaacccc gcctacgcga
     4261 acctcggggt cgccggaccg gacggcgcgg tcacctgtag cgcattcgcg atgccggccg
     4321 gaacgaggat cgaggaccgc ccctatttcc gcgatgcggt tgcgaccggc ggattctcga
     4381 tcggcgaata tcagatcggc cgcatcaccg ggacggcgtc gctcaacgtg ggccatccga
     4441 tcgtggccgg aggcgaactg cgcggcgtgg tcttcgcagc cattcgcctc accgccttga
     4501 acgatatcgc ccgtcgcacg gtgcttccgc ccggttccgt cgtgacgatc ctcgatcgaa
     4561 acggaacgat ccttgcgcgc gcacccgatc ccgagggata cgtcggcaaa cgattcgagg
     4621 actcgcgcct cgctgccgcg ctcgcgcggc gctccgggtc cacgctcgag gcgcacgggt
     4681 tcgacggcgt gaagcggctc tacggtcacg ttcggacggg cggctcggat ggcaacgaga
     4741 tcaccgtcgc ggtcggcatt ccgtcggcgg cggcgttcgc gaacgccaac cgagcgcgca
     4801 acgccgcgct ggccggcctg gcgatcgtca ccgtcctttc cgtgatggcg gggatgatcg
     4861 gcagccgccg gctcgtgcgg ccggttcgcc ggatggccga aaccgctcgc cggatcgaag
     4921 ccggcgacat gcaggcccgc tccgacctgc cggcgaccgg cgagctgggc gagctggctc
     4981 gatcgttcga cgacatggcc gtatcgctcg aacgccggct ctcggcgctg cgttacctga
     5041 cgcatcacga cacgctcacc gagcttccga atcgtcgcgc gctcgaagac gccatccgaa
     5101 ccggcgaatc gacgggcccc ggggcgttgc tgttgatcaa catcaaccgc ttccgtgacg
     5161 tcaatcacac gctggggcca cgcagcggcg atctcgtcct gcgcgaggcc gccgaccgga
     5221 tgcgttcgct catggagagc ggcgtgatgg tcgcgcgcgt cggcggcgac gagttcgcca
     5281 tcttgctcga gggcgtcgac cggcgcgaag cgacgatcgc ggctatggga gtcgtcgatc
     5341 agttcgaaac tccgtgcatg ctcggcgaca tcgaggtcga catgagaacc agggtcggcg
     5401 tggccccctt cgaacggccg gccgacccga acgaggtgat ccgccgcgcc gaggtcgccg
     5461 agcgaacctg ccgcgatcgc gggttgatct gtctcgtcta cagcgagcgc caggatccct
     5521 acaccgatca gcggcttcgc ctgatgggcg cgctccgcgg ggcggagagt cgcggccaga
     5581 tgcatctggt gttccagccc atggtcgaca cggtcacgag ccgcccggtt cgggtcgaag
     5641 cactgcttcg gtggcggcat cccgagctcg gcgaggtccc gcccgacctc ttcattccgc
     5701 tcgccgagga tttcgggctg atccggcggc tcaccacgtg ggtgctcggc gaagccgtgc
     5761 gcgaacacaa acgctgggcc tcggaaggga tcttccttcc ggtcgcggtg aacatctctg
     5821 cgatcagtct gaccgatccc gagctcaccg caacgatcga acaattgctt cgccgcgaag
     5881 gcgtcccgcc ggaggcaatc gaactcgaga tcacagagac ggccgcgatg gcacagcccg
     5941 aggaggcgct gcgaacgctc acgcacctgt cgtcgcgcgg aatccgcatc gcgatcgatg
     6001 acttcggcac cggacattcg tcgttgtcgt atctgagccg cctcccgatc gacacggtca
     6061 agatcgaccg gtccttcatc gcagcgctcg acggtgacga cgacgcgggc atcctggtac
     6121 gctcgacgat cgagctggca cacaagctgc ggcgaagcgt cgtcgcggaa ggcgtcgaag
     6181 acgagacgac acatcggatc ctgcaggcgg ccggctgcga ctgcatccag ggcttcttgg
     6241 tcggcacgcc gatgacctcg gatcgcttgg tcgagtgggc gcgcggcgtc gcgcctgcga
     6301 tggccggata gcccgccacg cccctccgct caaggtcacg gtggcgatgg gccggtcggc
     6361 cctcccccct gagggggtgt ctcgcgtagc ctcgggacga ggaggcagcc atgaccagcc
     6421 cacgcgccga aaccaccgcg cagacaccgt ccctggagca cgctgtcgcc gcgctcgaag
     6481 cgctcgtggg cgcaaagccg catcccgacg ccgtcccgaa ggcgcttcgc acggtgcggc
     6541 aggctctcga ggatcactgc gcgacgatgg aggagccgca aggaggattc gaccagatcc
     6601 tgagccacgc cccccacctg agccatgagg tcgccgggct tcggatcgag cacgccgcgg
     6661 cgcgcgcgtc gctggacaat ctcgtcgaga tgatcgatca cgcgccgagc ctgcaccacc
     6721 cggcgatgct ggtggcgatg atcgggcggg tccggctgct gatcgccgcg atcgagcggc
     6781 atcaccgaca cacggcacac acgctgcacg aggcattcgt ggccgagacg ggcgagatcg
     6841 actagcgaag cccgagctcg ccgcgcatgg ctgcggcgct gcgcgccgcg agggcgggca
     6901 cgccccattc gagctctttg aagagcgggt ggtcggtaat gccggcctga aagcggcggt
     6961 aggagccctc catgatgatc gcgatcttcc agccgcccag cgccgcatac caatcgatcg
     7021 ccgacacgtc ctctccggtc gcgccggcgt acgcctgcag cagctcgcgt cgccgcggaa
     7081 acccttcttg gtccatcacg ctcgacacgg tcaagccgaa cgagggatca ccgggatcac
     7141 gccaccaata aagcatccac ccgaggtcgg cgagcggatc gccgatggtc gacatctccc
     7201 agtcgacgac cgcgacgagg cgcgccggcg actcgggcgc gaagatcacg ttgtcgagct
     7261 tgtagtcgcc gtggacgatg gtggcgcggc gctgcgacgg gatgttctcc gagagccact
     7321 tcccgaccgc ttcgatgtcg ggcaggtcgc gaaagcgggc gagttcccag agctggttca
     7381 tgcggcgcaa ctgccgctcg aggtatccct cgggcttgcc gaagccctcc aggccggcgg
     7441 cgcggtagtc gatgctttgc agagccacga gcgtgtcgac cagggcacgc gccattcgcg
     7501 cacggtcatc cattgacgac agcgcctccg gcaactcggt cctcacgacc aggccgttca
     7561 ccggttccat cagatagaac ggcgcgccga tcacgccggt gtcggtgcat agcgcaaccg
     7621 gtggcggaac cggaaccggc gcgtcgtgcg aatgcagggc gctcatgaca cgatattcgc
     7681 gtgtgacgtc aaaggcgctc ggtggcagat cgccgcgcgg cgggcggcgc aaaaccacgc
     7741 gccagccctc gccttcgagc atgaacgtca agcacgactg cccttcgccg aggcgcgtca
     7801 cgccaaactc tcccccgtac tgtgggagac gggccgcgag gtagcgggcg atgccggccg
     7861 gatcgacggc gagcgcgtcc tccggcgcga tgtgcggcgt cacgtcttca tctcctcggg
     7921 gatgtagcgt gcgagctccc gccgggcgag caccatgaga tgcacgtcgt cgggcccgtc
     7981 ggcgattcgc agagtccggg cgcccgcgta catggcggcg agcggcacgt cgtcgctcac
     8041 gcccatcccg ccgtggatct ggatcgcccg gtcgatcacc gcgagcgcca tgcggggcac
     8101 cgcaaccttg atcgccgaga tctcgacccg cgcggcttcc ttgccgtgcg tgtcgatcag
     8161 ccacgcggcc ttgagcacaa gcaagcgcgc ctgctcgatc tcgatgcggg actcggcgat
     8221 ctggttctgg accacgccct gctgggcgag tcgcttgcca aacgcctcgc gggtgagcgc
     8281 gcgccggcac atcaggtcga acgcacgctc ggccatgccg atggcgcgca tgcaatggtg
     8341 gatccggccg ggcccgagcc gcgactgcgc gatgcggaag ccgccaccct cttcaccgag
     8401 caggttcgcc accgggacgc gcacgtcggt gaactcaatc tcgcagtggc cgtggccttc
     8461 gtcgtagccg aacacgggaa gcgtgcggat gacgtgcacg ccgggggtgt cgatgggaac
     8521 cagcaccatg gactgacgcg cgtaggagtc ggcgtcgggg ttggtgacgc ccatgaagat
     8581 gagtatcttg cagcgggggt cggcggcacc ggacgtccac cacttccgcc cgttgatcac
     8641 ataggtgtca ccatcccgct cgattcgggc cgagatgttc cgcgcatccg acgaggcgac
     8701 gtccggctcg gtcatcgcga acgctgagcg gatctcaccg ttgaggagcg gctcgagcca
     8761 ggttttcttc tgctcgtcgg ttccgaactc ggcgatgatc tccatgttgc cggtatcggg
     8821 ggcagcgcag ttgatcgcct cgggcgcgat gtgcgggacg cggcccgtga tctcggccag
     8881 cggcgcatac tcgctcacgc tcagtccggg accgtaggcg ggatcgggca tgaacaggtt
     8941 ccacagaccg cgagcgcgcg cttccttctt gaggtcgtcc atcaccggcg gactgtggtg
     9001 cggatcgggc agcgaagcga cctgctcgtg ccagatccgc tcggccggat agacgcgcga
     9061 atccatgaac tcgagcaggt tcttgcgaag ctcttcgacc ttcgggccga acgagaaatc
     9121 cactccctcc accccccgcg atgcgcagcg acgatcggtc aactatagcg atccttcact
     9181 cgccggaatc cggcgtgatt cgtcgagacg gcggaacacg gggttggcgg ccatcccggc
     9241 ggtccaaagg aggtaggcca cggcgatcgc gatcatcacg gtgcgaatgc ccgcacgctc
     9301 gatcagccat cccgccgtga tcgtcccggc ggggatcgac gcccacgaca gcgccagcgt
     9361 ggcaccgaac acccggccgc gaagccgctc cggcacgcgc tcgaactgga tcgccatgat
     9421 cacgggattg agcggacccg cggccacacc ctttaccgcc atcaccgcga ccaataccgg
     9481 caccggcgga aacgcgatga ggacccacag gaaggccgcc agcgtggcga agccccccac
     9541 gaagacccgg cggcgaggga tcgtcgatcc gcgccagccg aacagcaacg caccgacgac
     9601 cgcgccgccc cccgccgcgc cgagcatcag gccgagcacg acggggctgt cgtagatgcg
     9661 ttccgcgagc acgggcagca caacggcgta cagcggcgca tcgagcaggt tggtcaccat
     9721 cacggtgatg ccgatccagc gaatgaccgg gtctcgccag acgaagccga gcccctcgcg
     9781 catctcggcc cagtagcggc cgcgcccgtc cgagcgcaac gaggatccgg gaacggcgac
     9841 cgcgacggcg accgcggaca cgagaaacga cgccgcattg atccacaacg cggcgggcgc
     9901 acccgctgcg gcgatgatca ggccggcgag cggcgccccg gccagccggg acgatcgctc
     9961 gacaacctga atggaagcgg ttccgcgctc gatcgacatt cctgcatcct cagcgagccg
    10021 ggggacgagc gcctcgcgcg cgatcacccc gggggcgtcg agcagcgacc cggtgaagac
    10081 gagcatggcc agctgccaga actcgatacc gaccgtggca tgcagcagcg ggatcgccgc
    10141 gaccgtgact ccgctgagca catccgcgag cacgctcgtg cgcttgaacc cgagacggtc
    10201 gatcagcacg ccgccgaaca gcgacgcgac cacggtcggg aggaatgaca tcgccgcgat
    10261 gaagccggcg cgtgtcgcgc tgcccgtcgt gcgaagcacg aaccacggca cggcgatccc
    10321 cgcgatcacg ttgcccgcca tcgagacgcc gctcgacgac agcaacgcga tcagcggtcc
    10381 tcgggtgtgc attcccggct tcatggacac accaggatag agcggatcgg gtagcaagac
    10441 ccttcgattg cgggccgaac cggcctgccg catttgcgga cgacgtccgt aggatccaac
    10501 aagccgaagg aggttcccat gtcgaaggtc accgagtatc tccagcaaca ccatgtgccc
    10561 ttcaccaccc tggggcatgc ggccgccgca tcggcggtcg ccgaggcgta cgtgctcgtg
    10621 ctgccaccgt ggaaggtcgc caagacggtc gtgctcgaca cgagcaaagg gcatgtcatc
    10681 gccgtggttc cggctgaccg gcgtgtcgat ctgcctcggg ttcgggcggc gctcggggat
    10741 ccgcacgcga cgcttgccga cgaggccgag atcgcgcgtg actatcccga gttcgagctc
    10801 ggcgcgatcc cgccgatcgg acgcctgacc gggaccacca cgctcgtcga tcccgagatc
    10861 gcgggcctgg agacgatcgt gttcgcgtcg ggccggcaag acgagtccgt ccgcatcgct
    10921 accggtgatc tgctcgacga cgcgaacgtg cgggtcctgc ccatcgtcgt ggcccccacg
    10981 ttcgaggacg actggctcca gtaacccgga agcatgcgcc gcgcccccgc aaggggcgcg
    11041 gccttgcgtt ccgggctcaa acgggatgaa cgccggacag atctcgctcg gcgccgagaa
    11101 gatcccggag cgacacgatc ccggtcaatg ccccgccgtc cacgaccggc aggtgccgga
    11161 cgcgcagcgc caccatgatg cccagcgcgt gctccagatc cgcctccggg ccgatggtcg
    11221 ccggcgaggg tgtcatgtag tcaccggccg gggtcaactc ggtccgcgct ccgttggcga
    11281 cggcgcgcac gatgtcgcgc tcggtcagga ttccgaccaa tcgccctcct tcggtgaccg
    11341 gcagcgcacc cacgtcgaaa aaccgcatgc ggtcggcggc atctgcgagc gactcctcga
    11401 cgtcggccgt gaccgctttc tccaaagtga tctgtttgat cttcatcacg gtctcctcgg
    11461 ttcgggcctt cgtccatggt gggctcgccg gccggaatgc gcgagggtcg ccggtccctg
    11521 agagcggggg ccgcccggcc ctgctccccc cgcggcttcc gctgcagtgt ggagccagga
    11581 ggtggacttc gatgaaggta cgtgacgtta tgacagacga cgtggtcacg gtgaggccgg
    11641 acacgtcgtt tcgcgagatt gtgtcgctgc tgatggagcg cagggtgagc ggcgtcccgg
    11701 tcgtggacga aaccggcacg gtcgtggggc
//
LOCUS       DATLFO010000184        17499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_106147, whole genome shotgun sequence.
ACCESSION   DATLFO010000184 DATLFO010000000
VERSION     DATLFO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17499
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106147"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VM841_07335"
     CDS             complement(<1..544)
                     /locus_tag="VM841_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90030.1"
                     /translation="MTVPHAALKVYEPLESFEPAERERWLAAAEAMSASRGPSTLVLS
                     RAHGLSGADPGERALVIRYGDRHYACPHRTRLRLLASLVAFRRTIPGEVAGAFMPDGE
                     AERAIRELEALRAQHPDWRSHILTSTWEVPTRWFVLFDDEERIFDPEGPSLVYRTTMS
                     AARLRTERALEAAKQAMIGPG"
     gene            644..1678
                     /locus_tag="VM841_07340"
     CDS             644..1678
                     /locus_tag="VM841_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVL90031.1"
                     /translation="MERKRRPYSTGDATLDARVQEMVEQMSLRDSDLVFEIIATAIRL
                     GRDEATRLDRKIVNSALKEMRYAYRVFAPYRDVRKVSMFGSARTPADEPEYQTSREFA
                     KRIVDRDWMVVSGAGPGIMEAANEGAGAAKSFGVNIRLPFEAKANPSIADDPKLINFK
                     YFFTRKLMFIKEGDAFVLLPGGFGTLDELFELLTLVQTGKSDPHPIVLLDKPGGTYWK
                     GWDSFVREHLISRGLISADDLNIYRLTDSIDEAVDELVTFYRNYHSIRFHGRTLILRV
                     QRVPDEESLAALSRDFSDILRDGQITPTEPLKEEIRDDDALTLKRLQVPFDQMSYGRL
                     RTLIDRLNEF"
     gene            1839..2900
                     /locus_tag="VM841_07345"
     CDS             1839..2900
                     /locus_tag="VM841_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HVL90032.1"
                     /translation="MDVFESLTGEYEQIVYGHNPDTGLRTIIAIHSTALGPGLGGTRF
                     YPFPSEEAALRDVLRLSKGMAYKNAAAGLDLGGGKAVIIGDPATDKTPDLLRAYGRVV
                     DRLGGAYVTTADVGTNSADMVFIAETTKHVTGTPDASGDPSPNTAYGVWSGMRAIAER
                     LWDEPSMKGKRVAVQGVGKVGAELARLLADEGAVVAVADIDAVAAGKLAAELGADVLD
                     AADVLAAECDVLAPCALGAVVNDASLPRFRCRAIAGAANNQLERPEHGEALRTAGILY
                     APDYVINAGGVISVAGEVMGYGADEARARAAKIADTLRDVFGEADAQGVSPALTADVL
                     AERRVRSAERTFGGPGGSR"
     gene            2967..3485
                     /locus_tag="VM841_07350"
     CDS             2967..3485
                     /locus_tag="VM841_07350"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HVL90033.1"
                     /translation="MPPTTAPSNDIEKKARRVLQQSGVKEVPVDLERVLAHLRLHHER
                     PWHLSTDVWDALVRKDRGRTRFKGQAMRATSRERWRLAHEIGHHVLHGAEPIRAHGQW
                     EKNELEHEADLFAAELLMPPPLISKAVKQAERAHQFDIEEMAHEFKVGRHEMEKRLRQ
                     LGLLTKEVLGRM"
     gene            3684..4778
                     /gene="pdhA"
                     /locus_tag="VM841_07355"
     CDS             3684..4778
                     /gene="pdhA"
                     /locus_tag="VM841_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HVL90034.1"
                     /translation="MRGVVDPASIPTDSVEILKPDGTLVPDHGYQLDLKEEDLREIYR
                     AMMLARRADQESTNLQRQGELGVYTPCLGQEGAQVGAGYALEQSDWVFPSYRELAIAM
                     MRKIDPAEILHMFRGTWHGGLWDPREHHYMYLSVPIATQMLHAVGFAYAARMKGEKTV
                     TLACCGDGATSEGDFHEACNYAGVWKTPTVFLVQNNQYAISVPLAKQTAAPTIAAKAL
                     GYGFPGVRVDGNDVLACYAVTKEAVRRAREGHGPTLIEAVTFRMGPHSTADDPSRYRS
                     KEEEAGWKARDPIDRFTKYAEAEGALTDEFKKQVEEEAKEIARHIRAEIVGAGPGPVD
                     ELFENVYSEMPQILKQQHEEFKRIQREREG"
     gene            4784..5758
                     /locus_tag="VM841_07360"
     CDS             4784..5758
                     /locus_tag="VM841_07360"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HVL90035.1"
                     /translation="MASVTLAQSLNEALRASLRDDDRVVVMGEDVGINGGVFRITDKL
                     QAEFGEERVIDTPLAESGIVGTAIGMAMYGLKPVCEMQFDGFTYPAFEQLVSHLAKFG
                     MRSKGKFRPSVVVRIPYGGGIGAVEHHSESPEAYFAHTAGLRVVCPSTPSDAYSLLIE
                     SIQSPDPVIFFEPKRRYWIKEEVDLPTTTQPMGNAVVRREGTDVTLISYGPMMRVALD
                     AAEEASSDGVGVEVIDLRTISPLDVGTVLESVRKTGRAVIVHEANVSFGVGAELAARI
                     AEADFTSLEAPVIRVGGWDMPYPPAKLEEEYLPDVDRVLDALDAVLNY"
     gene            5785..7185
                     /locus_tag="VM841_07365"
     CDS             5785..7185
                     /locus_tag="VM841_07365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HVL90036.1"
                     /translation="MAREFRLPDLGEGLTEAEIVSWLVKEGEEVALNQPLVEVETEKA
                     LVEIPSPFEGVVAKLHSEVGDKVEVGSVIVSFDADGADGEPEVAGDRKEGKKEGGRRE
                     VLVGYGPEEEGAGGRRRRRKIGKRGADEPAAEEPEEPDATEAEEEPEPEPEPAKAPAP
                     AAKKSGATVRALATPPVRKLAKDLGIDIDELGGTGPGGRVTREDVMAAAKGDGSPAKK
                     TEPAAAAAAKQTHPAGGEERIPVRAIRRSIAEKMVRSYSEIPHVTEWLEVDATELMRL
                     RKDLAGSPEATDGKMSPLPVIVKALVAALRKHPLINSAWDSETNEIIVKHYYNVGIAA
                     DTERGLLVPVIRNADQMNVFEISDEIRRLAVAARDKTIGPNELSGSTITITNIGSFGM
                     DGGTPIINYPESSILAPGAIRQKPWVVDGKIEVREVMTLGLSFDHRIVDGAEAGRFLR
                     YLADLIEKPARLFGVL"
     gene            complement(7188..7919)
                     /locus_tag="VM841_07370"
     CDS             complement(7188..7919)
                     /locus_tag="VM841_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90037.1"
                     /translation="MRPGLVSPSAVPLDVDRGENHVPDAVATIAHELKTPLASILGYA
                     RMLRAAGDTLDPRRRDEFFEVVEQQGRRLTHMIDDLLQFARTDAGPQKLSRTPVDIAG
                     VVDRAVSTVSGLSGDHIIHTSVPDGDLGLYGDAAALEHVLTNLLENAIKYTPDATVIE
                     VVVAGHRHEIRISVADKGPGIDPADLPYVFDRFRRGSGVVRGVGLGLHIVRTLVEAHD
                     GRVWAESPPGEGATFTVALPRRAVR"
     gene            complement(8049..9083)
                     /gene="purM"
                     /locus_tag="VM841_07375"
     CDS             complement(8049..9083)
                     /gene="purM"
                     /locus_tag="VM841_07375"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HVL90038.1"
                     /translation="MTTPPKTTTYAGAGVDIEAGDKAVEMIKDVVASTARPEVLGALG
                     GFSGAFVPDFSRFTTPVLLSSTDGVGTKLAIAQTLDKHDTVGIDLVAMVVDDLVCDGG
                     EPLFLLDYIACGRLVPERIAAIVEGIAEGCRQAGCALVGGETAEHPGIMDPDHYDLAA
                     FAVGVADRDDMPGPHRVGAGDVLIGLRSSGPHSNGYSLIRRLILEHDLDLSGDPANLG
                     TSLGEALLTPTAIYAPEILLAMRTGAVHAAAHITGGGLAGNLVRALPEGAKAVVHRDA
                     IPVPPLFEWLGRLGRIEDAEMRKTFNMGIGMVLVAAEHAVQAVLDATAMHHPAVIGEI
                     VPGARAVDLV"
     gene            complement(9080..10507)
                     /gene="purF"
                     /locus_tag="VM841_07380"
     CDS             complement(9080..10507)
                     /gene="purF"
                     /locus_tag="VM841_07380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HVL90039.1"
                     /translation="MGADSPKEECGLFGVWAPGEEVAKLTFFGLYALQHRGQESAGIA
                     VSDGNGIIVYKDMGLVAQVFSEATLATLSGPLAIGHTRYSTTGSTRWENAQPTFKPTG
                     SGGGLALGHNGNLTNTRALAAKVTRKTNGPKASTDSDIVAELLAERTGEMPLEDALMS
                     LMPELAGAFSFVVMDERTVYGVRDSYGIRPLSIGRLANGGWVLASETCALDIVGAAYV
                     RDVEPGEIVAVDDRGLRSWIYAKPTPKLCIFEYVYLARPDSLLYEHGVHEVRREFGRR
                     LADEAPVEADLVIGVPDTGPVAAAGFAEATGIPYGDGIVKNRYVGRTFIQPTQTIRQM
                     GIRLKLNPLRSAIKGKRIVVVDDSIVRGNTTRQLVALLREAGATEVHLRITSPPVRWP
                     CFYGIDMATKAELIASDLAVDEIRAYVGADSLAYLSLEGLVAATGNDKNRFCRACFDG
                     IYPVPIPQADDESHKFVLEEPSPQQ"
     gene            complement(10523..12718)
                     /gene="purL"
                     /locus_tag="VM841_07385"
     CDS             complement(10523..12718)
                     /gene="purL"
                     /locus_tag="VM841_07385"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HVL90040.1"
                     /translation="MSATLIERASALGLTEGELNKIVETLGRDPSPAELAMYAAMWSE
                     HCSYKSSKVHLKKLPTTGPRIVMGPGENAGVVDIGDGLVAVFKLESHNHPSAVEPVQG
                     AATGVGGIVRDILATGARPVALLDPLRFGDPAEWRQRHLLGGVVQGIGQYGNSIGIPT
                     VGGEIKFDPCYAGNPLVNVMCIGIMRKDLVARARAQGEGNVAILYGAKTGRDGIGGVS
                     VLASRPFDAEAEAKRPSVQVGDPFSEKVVIEATLELLEQGLIVGVQDLGGAGFCCATS
                     ETASRGEVGMEIDLDAVLLREPDMEPFEILTSESQERMFAIVKPEDVERAVAVCERWG
                     VDAMIVGKVTAGDRLVIKQLGEVVADIPARSLADDGPTYNRPMERPDWIDALHADDPA
                     GLPAPKNIGEALLTVLGAPNVCDKSWAWEQYDYLVQHNTLQGPGGDAAVVRVEGTPRA
                     LVISTDGNGRYGQLDPRLGGMHAVAESARNVACTGARLLAISNCLNFGNPERPDVMWQ
                     FAEATQGMSEACLALETPVTGGNVSFYNETDGTSIHPTPVVGMLGILEDATIHTGIAF
                     GGEGDAIVLLGETFDELGGSEYAWAVHRHLGGHPPKLDLTMEKALCDLLATLAEGRLL
                     VSAHDCSEGGLAVTLAESCMAGEIGARVSLESALAPHVLWFSESASRALVSCPASGLE
                     AVLVAAASAGVPATAIGTVGGESLACGDFDLPVSALRSAWKRTLPDLLA"
     gene            complement(12715..13386)
                     /gene="purQ"
                     /locus_tag="VM841_07390"
     CDS             complement(12715..13386)
                     /gene="purQ"
                     /locus_tag="VM841_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVL90041.1"
                     /translation="MKIGVIQFPGSLDDRDAVWAFEKLGAQAELIWHGDHDLRGADAI
                     IVPGGFSYGDYLRCGAIARFAPVMEQVAAFARDGGIVLGICNGFQVLCEAGLLPGALV
                     RNEGLKYRCVPVTLRVESNRSPHLSGTNAGDTLRIPIKHGEGRYVADAGTIARMEANG
                     QVALRYDGENPNGSVAGIAGVTNEAGNVLGMMPHPEHAVDDTVGIRSADGAIILGSIL
                     KAVAR"
     gene            complement(13396..14226)
                     /locus_tag="VM841_07395"
     CDS             complement(13396..14226)
                     /locus_tag="VM841_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90042.1"
                     /translation="MSTTTDEERSASEQDYRSYRRQRRQERRAARAAKKAAKPKGIDL
                     PFRKAMHPALLAAFAVVALTGIVLTVVMLINPPATEIAIAERRSPPSKTLSHDVGQIV
                     LAPIPEDLPVTRAPCRAVEGVVIEGGEPAHVRLGGVLQRLCPHAAGESDVARSIRALS
                     EARLRFAVFQRSGDFSTLSSADPVRILINIRFAQRDVSAALIGPLLVHEGFHLLHPDE
                     PLTAELEFRAREAELQACRLLIDIEAWDRGCTDAQRLVEFGRERAIQQLVAAGFPRGA
                     "
     gene            complement(14223..14480)
                     /gene="purS"
                     /locus_tag="VM841_07400"
     CDS             complement(14223..14480)
                     /gene="purS"
                     /locus_tag="VM841_07400"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009189438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HVL90043.1"
                     /translation="MKVRVLLDVMLKDGILDPQGQTVERNLPSLGFKGVSGVRIGKHI
                     VFDIEAESREAVDAQVRDMAEKLLSNPVIEDFTYRIDESGE"
     gene            complement(14477..15385)
                     /locus_tag="VM841_07405"
     CDS             complement(14477..15385)
                     /locus_tag="VM841_07405"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVL90044.1"
                     /translation="MTLAGLESIRKGKVRELYRTSEGILLVASDRISAYDVVLPTPIP
                     DKGSVLTGLSKFWFGRFDDIIGNHLIATAPEDFPPEAKQHADALRGRAMLVKETTVVP
                     IECVARGYLSGSGWKEYKANQTVCGIKLPAGLQESDKLPEPIFTPATKAEAGEHDENI
                     SFERACEIAGKETMEKLRDATLTIYSEAADYARSKGIIIADTKFEFGTLPSGELILID
                     EVLTPDSSRFWPADQYEPGKSPPSFDKQYVRDWLDASGWDHTPPGPELPDDVVASTRA
                     KYAEAFEVVSGASFDRYLATAELEPK"
     gene            complement(15382..16683)
                     /gene="purB"
                     /locus_tag="VM841_07410"
     CDS             complement(15382..16683)
                     /gene="purB"
                     /locus_tag="VM841_07410"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HVL90045.1"
                     /translation="MIPRYSLPEMAAVWEERSKYRRWLQVELLAVDAWAKLGVVPAED
                     ATTIRDNAAEVDPVRVEEIEATTNHDVIAFLTAMGEKIGPASRWIHYGMTSSDLLDTA
                     LALQMRDACDILLAKTDHLLEAVKRRAIEFKDAVCVGRSHGVHAEPTTFGLKMAVWAF
                     EIARDRERLRRARDIVSVGKIAGAVGTYASIDPFVQDYVCAELGLSSAEAATQVIQRD
                     RHAELMAACAITAGTLDKIATEIRHLQRTEVREAEEPFRKGQKGSSAMPHKRNPIICE
                     RVSGLARVVRAAVVPAIENMALWHERDISHSSVERVMMPDATIALDYILKLTTDVIEG
                     MTVDVERMRQNLDATGGLVYSGSMLLALVEAGMTREDAYAAVQTAAMRTWETGTPFAD
                     TLMDVPEVTAVASRDDLARIMDPSRYLTHVDVVFKRLEGIE"
     gene            complement(16680..17144)
                     /gene="purE"
                     /locus_tag="VM841_07415"
     CDS             complement(16680..17144)
                     /gene="purE"
                     /locus_tag="VM841_07415"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVL90046.1"
                     /translation="MSVQVGIVFGSPSDRDKMAPAGVVLEKFGIEFEMEAMSAHRQPA
                     RVHEYVTSAESRGLRVMIAGAGRAAHLPGVVASLTPLPVIGVPLSGGIMDGMDALLSV
                     VQMPSGVPVGTVAVDAASNAGLLAVQILAAGDDDLRKKLHEYKQQLAEGLRL"
     gene            complement(17141..>17499)
                     /locus_tag="VM841_07420"
     CDS             complement(17141..>17499)
                     /locus_tag="VM841_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HVL90047.1"
                     /translation="ACADGSLDRAAARLSDRTAVCVVLAGEGYPARSDTGTPIDGLDA
                     AAQVKDVTVYHAGTARDGGRVVTNGGRVLGVTGMGDDVASARDRAYEAASKISFNGMQ
                     YRRDIGSRAMGGERKL"
BASE COUNT         2881 a         5859 c         6002 g         2757 t
ORIGIN      
        1 cgccgggacc gatcatcgct tgcttggcgg cttcgagcgc ccgttcggtg cgcaagcggg
       61 ctgccgacat cgtggttcgg tacaccagag acggaccttc cgggtcgaag attcgctcct
      121 cgtcgtcgaa aagcacgaac caacgcgtcg ggacctccca cgtcgaggtc aggatgtggc
      181 tccgccagtc gggatgctgc gcgcgcagcg cctcgagctc gcggatcgct cgctcggcct
      241 cgccgtcggg catgaacgcg cccgcgacct cgccgggaat ggtccgccgg aacgccacca
      301 gcgaagccag cagccggagc cgcgttcgat gagggcaggc atagtgccgg tccccgtagc
      361 ggatcaccaa ggcgcgctcg ccgggatcgg caccggacag gccgtgcgcg cgtgagagca
      421 ccagcgtcga aggaccgcgc gaagcggaca tcgcttctgc ggctgcgagc catcgctccc
      481 gctcggccgg ctcgaacgac tcgagcggct cgtagacctt gagcgccgcg tgcggaacgg
      541 tcatcccgac cgatcctata ccccgcctgc gaagaaccaa cagaagcgtt ttccgcgcca
      601 agcgagcgac cgcgcgttcg cggacggata cgatgcagcc cttatggagc gcaaacgacg
      661 tccctattcg accggcgacg cgacgctgga cgcgcgcgtg caagagatgg tcgagcagat
      721 gagcctgcgc gactccgatc tggtcttcga gatcatcgcg accgcaatcc gcctgggccg
      781 cgacgaagcg acgcgcctgg accgcaagat cgtcaactcg gcgctcaaag agatgcgcta
      841 cgcatatcgc gtgttcgccc cgtatcgcga cgtgcgcaag gtcagcatgt tcggctcggc
      901 gcgcaccccc gccgacgagc ccgagtatca gacgtcccgc gagttcgcga agcgcatcgt
      961 ggaccgcgac tggatggtcg tgtcgggcgc gggcccgggc atcatggagg ccgccaacga
     1021 aggcgccggc gcggccaagt ccttcggcgt gaacatccgc cttccgttcg aggcgaaggc
     1081 gaacccgtcg atcgccgacg atccgaagct gatcaacttc aaatatttct tcacgcgcaa
     1141 gctgatgttc atcaaagaag gcgatgcctt cgtgctgctg cccggcggat tcggcacgct
     1201 cgacgagcta ttcgagctgc taacgctggt gcagaccggc aagagcgacc cgcatccgat
     1261 cgtgctgctg gacaagccgg gcggcacgta ctggaagggc tgggattcgt tcgtccgcga
     1321 gcatctgatc tcgcgcggct tgatctcggc cgacgatctg aacatctacc ggctgaccga
     1381 ctcgatcgac gaagccgtgg acgagctggt gacgttctac cgcaactatc actcgatccg
     1441 gttccacggc cgcacgctga ttctgcgcgt gcagcgcgtc cccgacgagg aatcgttggc
     1501 cgcgctttcc cgcgatttct ccgacatctt gcgcgacggc cagatcaccc cgacggagcc
     1561 gctcaaagaa gagatccgcg acgacgacgc cctgacgttg aagcgtctgc aagtcccgtt
     1621 cgaccaaatg agctacggcc gtctgcgcac cctgatcgac cgactcaacg aattctgacc
     1681 gatccgttcg ctgtctctcg gcatccgggt gctggccgga gacgccggcg cgccagaggg
     1741 gcctcagcga cgaaataccc gaacgaacga cggccgcgtg tccgcgccct tcaataacac
     1801 ggggggatgg gccggccggg gtctcgtatc atggcgctat ggacgtcttc gaatcgctta
     1861 ccggcgaata cgagcagatc gtctacggac acaatccgga caccggtctg cgcaccatca
     1921 tcgcgatcca ctcgaccgcg ctggggcccg gcctcggcgg gacccgtttc tatccgttcc
     1981 cgtccgaaga agcggcgctg cgcgacgtgc tgcgcctatc caaagggatg gcctacaaaa
     2041 acgcggccgc cggcctggac ctgggcggcg gcaaggccgt gatcatcggc gatccggcca
     2101 ccgacaagac tcccgacctg ctgcgcgcct acgggcgtgt ggtggaccgg ttgggcggcg
     2161 cctacgtgac gaccgccgat gtcgggacca attcggcgga tatggtcttc atcgccgaga
     2221 caaccaagca cgtgacggga acgccggacg cgtcgggtga cccgtcgccc aacaccgcct
     2281 acggcgtctg gtcgggcatg cgggcgatcg ccgagcggct ttgggacgag ccgtcgatga
     2341 agggcaagcg cgtggccgtg cagggcgtcg gcaaggtcgg cgcggaattg gcgcggctgc
     2401 tcgccgacga gggggccgtg gtcgccgtcg ccgacatcga cgcggtcgca gccggaaaac
     2461 tcgcggccga gctcggagcc gacgtgctgg acgccgccga cgtgctcgcc gccgaatgcg
     2521 acgtgctcgc gccgtgcgcg ctgggtgcgg tcgtcaacga cgcctcgctg ccgcgctttc
     2581 gatgccgtgc gatcgccggg gccgcgaaca accagctgga gcggcccgag cacggcgagg
     2641 cgctgcgaac ggccgggatc ctgtacgcgc ccgattacgt gatcaacgcc ggtggtgtga
     2701 tcagcgtcgc cggcgaggtc atgggctacg gcgcggacga ggctcgcgcc cgggcggcaa
     2761 agatcgccga cacgctccgc gacgtcttcg gcgaggcgga cgcgcagggc gtgtcgcccg
     2821 cgctgaccgc ggacgtactc gccgaacgcc gggtccgatc ggccgagcgc acgttcggag
     2881 gcccgggcgg gtcgcgctag cgctttgttg cacggcgagg gccgcgatag gttgacccgg
     2941 acgattcgac caaggagcct gtgaacatgc cgccgacgac cgccccctcg aacgacatcg
     3001 agaagaaagc ccgacgcgtc ctgcagcagt cgggtgtcaa agaggtgccg gtcgacctgg
     3061 agcgcgtgct ggcccacctg cggctccacc acgagcggcc ctggcacctg tccaccgacg
     3121 tctgggacgc tctggtccgc aaggaccgcg gccgcacccg gttcaagggc caggccatgc
     3181 gcgcgacctc gcgggagcgc tggcggctgg ctcacgagat cggccaccac gtcctgcacg
     3241 gggccgagcc gattcgcgcc cacggacagt gggaaaagaa cgagttggag cacgaggccg
     3301 acctattcgc cgcagagttg ctcatgccgc ctccgttgat ctcgaaggcg gtgaagcagg
     3361 cggagcgggc gcaccaattc gacatcgagg agatggccca tgaattcaag gtcgggcgtc
     3421 acgagatgga gaagcggctg cgccagctgg gcttgctcac caaggaagtt ctgggccgca
     3481 tgtaacagtt ccgcgacaac ggcgcgggga gtgccggaaa cgtcccttat gcctttgacg
     3541 gggacgagcc cggcctgcga ccatgagtcg agccacagcg gcgaccccgc taggcgccga
     3601 ctccaactgc atcgatcctg ctcggcgatc ccgcgcaggg acgctccggc gatccggccg
     3661 ggcagggtga gggaggaaac cgaatgagag gcgtggtcga ccccgcgtcc atccccacgg
     3721 actcggtcga gatcttgaag ccggacggga cactcgttcc cgatcacggc tatcagctcg
     3781 acctcaaaga agaagacttg cgcgagatct accgcgcgat gatgctggcg cgccgcgccg
     3841 accaggaatc gaccaacctg cagcgccagg gcgagctggg cgtctacacg ccgtgcctgg
     3901 gccaggaagg cgcgcaggtc ggggccggct acgcgctgga gcagagcgac tgggtgttcc
     3961 cgtcctaccg cgagcttgcg atcgcgatga tgcgcaagat cgacccggcc gagatcctcc
     4021 acatgttccg cgggacctgg cacggcggcc tgtgggaccc tcgcgagcac cactacatgt
     4081 acctatcggt tccgatcgcc acgcagatgc tgcacgcggt cggcttcgcc tacgccgccc
     4141 ggatgaaggg cgagaagacg gtcacgctcg cgtgctgcgg cgacggcgcg accagcgaag
     4201 gcgacttcca cgaggcgtgc aactacgcag gagtctggaa gaccccgacg gtgttcctcg
     4261 tgcagaacaa ccagtacgcg atcagcgtgc cgctggcgaa gcagacggcg gctccgacga
     4321 tcgcggcgaa ggcgctcggc tacgggttcc cgggcgtgcg cgtggacgga aacgacgtgc
     4381 tggcctgcta cgcggtcacg aaggaagcgg ttcgccgcgc gcgcgaagga cacggcccga
     4441 ccttgatcga ggccgtcacg ttccggatgg gaccgcactc gacggccgac gatccgtcgc
     4501 gctaccgctc gaaggaagag gaagccggct ggaaggcgcg ggatccgatc gaccgcttca
     4561 ccaagtacgc ggaagcggaa ggtgcgctca ccgacgagtt caaaaagcag gtcgaggaag
     4621 aggccaagga gatcgcgcgg cacatccgcg ccgagatcgt cggcgccggc cccggcccgg
     4681 tggacgagct gttcgaaaac gtctactcgg agatgccgca gatcctgaag cagcagcacg
     4741 aagaattcaa gcgcatccag cgcgagcggg agggctaggc gatatggcat cggtcacgct
     4801 tgcgcagtcg ctcaacgaag cattgcgcgc atcgctacgc gacgacgacc gcgtcgtcgt
     4861 catgggcgag gacgtcggca tcaacggcgg cgtcttccgc atcaccgaca agctgcaggc
     4921 cgagttcggc gaagagcgcg tgattgacac gccgctcgcc gagtcgggca tcgtcggtac
     4981 cgcgatcggc atggcgatgt acggcctgaa gccggtctgc gagatgcagt tcgacgggtt
     5041 cacctatccc gccttcgagc agctggtgtc gcatctggcg aagttcggca tgcggtcgaa
     5101 gggcaagttc cgtcctagcg tcgtcgtgcg catcccctac ggcggcggca tcggcgcggt
     5161 cgagcatcac agcgagtccc ccgaggcgta cttcgcgcac acggccgggc tccgcgtggt
     5221 ctgcccgtcc acgccgtcgg acgcgtactc actcctgatc gagtcgatcc agtcgccgga
     5281 cccggtgatc ttcttcgagc cgaagcgccg ctactggatc aaggaagagg tcgatcttcc
     5341 gaccacgacc cagccgatgg gcaatgcggt cgttcgccgc gaaggaaccg acgtaacgct
     5401 catctcgtac ggtccgatga tgcgggtcgc actcgacgcg gccgaggagg catcgtccga
     5461 cggggtcggc gtcgaggtga tcgatcttcg cacgatctcg cccctggacg tcggcaccgt
     5521 gctcgagtcg gtccgcaaga ccggccgtgc ggtgatcgtg cacgaggcga acgtgtcctt
     5581 cggcgtcggc gccgagctgg ccgcgcgcat cgcggaggcc gacttcacct cgctggaggc
     5641 gccggttatc cgcgtcgggg gttgggacat gccgtatccg ccggccaagc tcgaagagga
     5701 gtaccttcct gatgtggacc gcgtgctcga tgcgctcgac gcggtcctga actactagac
     5761 gacaaccgac ccgggggttc gacgatggcg cgtgagttcc ggcttcccga tcttggcgaa
     5821 ggcctgaccg aggccgagat cgtttcgtgg ctggtcaagg aaggcgaaga ggtcgcgctg
     5881 aaccagccgc tggtcgaggt cgaaaccgag aaggcccttg tcgagattcc ctcgccgttc
     5941 gaaggcgtgg tggccaagct tcacagcgag gtcggcgaca aggtcgaggt cggctcggtc
     6001 atcgtaagct tcgacgcgga cggcgccgac ggcgagcccg aggtcgcagg cgaccggaag
     6061 gaagggaaga aggaaggcgg ccgccgtgag gttctcgtcg gctacggccc cgaggaagaa
     6121 ggagcgggcg gccgtcgccg ccgccgcaag atcggcaagc gcggcgccga cgagcccgca
     6181 gctgaagagc ccgaggagcc cgacgccacg gaagccgaag aagaacccga gcccgagccc
     6241 gaacccgcga aagcgcctgc gccggctgcg aagaagagcg gcgcgacggt gcgtgcactg
     6301 gccaccccgc cggtccgaaa gctcgcgaag gatctgggca tcgacatcga cgagctcggt
     6361 ggcaccggcc cgggtggccg cgtgacgcgc gaagacgtga tggccgccgc caagggagac
     6421 ggctcgcccg caaagaagac agagccggcc gccgcggctg ccgcgaagca gacacacccg
     6481 gcgggcggcg aggagcgcat cccggtgcgc gcgatccggc gctcgatcgc cgagaagatg
     6541 gttcgctcct acagcgagat cccgcacgtc accgagtggc tcgaagtcga cgcgaccgag
     6601 ctgatgcgtc tgcgaaagga tctggcagga tcgcccgaag cgaccgacgg caagatgtcg
     6661 ccgctgccgg tcatcgtcaa agcgctcgtc gccgcgctgc gcaagcatcc gttgatcaac
     6721 tcagcatggg attccgaaac caacgagatc atcgtcaagc actactacaa cgtcggcatc
     6781 gccgccgaca ccgagcgcgg cctgctggtc ccggtgattc gcaacgccga tcagatgaac
     6841 gtttttgaga tctccgacga gatccggcgg ctggccgtcg cggcgcgcga caagaccatc
     6901 ggaccgaacg agctgagcgg gtcgacgatc acgatcacca acatcggatc gttcgggatg
     6961 gacggcggca cgccgatcat caactatccc gagtcgtcga tcttggcgcc cggcgcgatc
     7021 cggcagaagc cgtgggtcgt ggacggaaaa atcgaggtac gcgaggtcat gacgctcggc
     7081 ctgtcgttcg atcaccgcat cgtcgacggt gccgaggccg gacggttcct gcgctacctc
     7141 gccgacctga tcgaaaagcc cgcgcggctg ttcggcgtcc tgtagggcta gcgcaccgct
     7201 cgccgcggca gcgccaccgt gaacgtcgcg ccttccccgg gcggggactc tgcccagacg
     7261 cgtccgtcgt gcgcctctac gagcgtgcgc acgatatgca ggcccaggcc cacaccgcgt
     7321 acgacgccgc tcccacgccg gaaccgatcg aacacgtagg gaaggtcggc gggatcgatg
     7381 ccgggtcctt tatcggcgac cgagatgcgg atctcgtggc gatgcccggc cacgaccacc
     7441 tcgatcaccg tcgcgtccgg cgtgtacttg atcgcgttct ccagcaggtt ggtcaggacg
     7501 tgctcgaggg ccgcggcgtc gccgtacaaa cccaggtccc catcgggcac ggatgtgtgg
     7561 atgatgtggt cccccgaaag acccgagacg gtactgaccg cacggtccac gacgcccgcg
     7621 atgtcgaccg gggtgcggct cagcttctgc ggaccggcgt ccgtgcgggc gaactggagc
     7681 aggtcgtcga tcatgtgggt gagccggcgg ccctgctgct cgacgacctc gaagaattcg
     7741 tcgcgccgtc gcggatcgag cgtgtcgccg gcggcgcgaa gcatgcgcgc gtatcccagg
     7801 atcgatgcga gcggtgtctt gagctcgtgc gcgatggtgg cgaccgcgtc gggaacgtgg
     7861 ttctcgcccc gatcgacgtc gagcggaaca gcggatggcg atacgaggcc cggcctcacg
     7921 atggatcccc ccttgtgccc ttcagagggt cctccctttg aacccccttt agtgaatcgg
     7981 cctgggatcg gggtgaatga agggtcccgg cacattttcg ggagcctgcc cgaaacgtgc
     8041 cgtatgcgct acaccagatc gacggcccgg gcgcccggca cgatctcgcc gatcacggcc
     8101 gggtgatgca tcgcggtcgc gtcgagcacc gcctgaaccg cgtgctcggc ggcgacgagg
     8161 accatgccga tgcccatgtt gaaggtcttg cgcatctcgg cgtcctcgat gcgtcccagg
     8221 cggccgagcc attcgaacag cggcggaacc gggatcgcgt cgcggtgcac gaccgccttc
     8281 gcgccctcgg gcagcgcgcg cacgaggttg cccgccagtc cgcccccggt gatgtgcgct
     8341 gcggcgtgca cggcgccggt tcgcatcgcc agcaggatct cgggcgcata gatggccgtg
     8401 ggggtgagca gcgcctcgcc gagcgaggtc ccgaggttcg ccgggtcgcc ggacaggtcg
     8461 aggtcgtgct cgaggatgag ccggcgaatg agcgagtagc cgttcgagtg cggaccggac
     8521 gatcgcagtc cgatgagcac gtcgccggcc ccgacgcgat gcggtccggg catgtcgtcc
     8581 cggtcggcga ccccgacggc aaatgcggcc aggtcgtagt gatcgggatc catgatgccg
     8641 ggatgctcgg cggtctcgcc gccgacgagc gcacagccgg cctgacggca accctctgcg
     8701 atcccttcga cgatcgcggc gatgcgttcc ggcacgagac ggccgcaggc gatgtagtcg
     8761 agcaggaaca gtggctcacc accgtcgcag accagatcgt ccacgaccat cgcaaccagg
     8821 tcgatgccca cagtgtcgtg cttgtcgagc gtctgcgcga tcgcaagctt ggtgcccacg
     8881 ccgtcggtgg aggacagcag caccggcgtg gtgaaccgcg agaagtcggg gacgaatgcg
     8941 ccgctgaagc cgccgagcgc gccgagcacc tcgggccgcg cggtggacgc gacgacatcc
     9001 ttgatcatct cgacggcctt gtcaccggct tcgatgtcga cgccggcgcc ggcgtacgtg
     9061 gtcgtcttcg gcggcgtcgt cactgctgcg gcgaaggctc ttcgagcacg aacttgtgcg
     9121 actcgtcgtc ggcctgcgga atcggcaccg ggtagatgcc gtcgaagcag gcccggcaga
     9181 accggttctt gtcgttgccg gtcgcggcga cgagcccttc gagcgacagg taggcgagtg
     9241 agtcggcgcc gacgtaggcg cggatctcgt ccacggcgag gtcgctggcg atcagttcgg
     9301 ccttggtggc catgtcgatg ccgtagaagc aaggccagcg caccggcggc gaggtgatcc
     9361 gcagatgaac ctcggtcgcg ccggcctcgc gtagcagtgc gacgagctgg cgggtggtgt
     9421 tgccgcgcac gatcgaatcg tccacgacca cgatgcgctt gcctttgatc gcactgcgca
     9481 gcgggttgag cttgaggcgg atgcccatct ggcggatcgt ctgcgtgggc tggatgaacg
     9541 tgcggccaac gtagcggttc ttcacgatgc cgtcgccgta ggggatcccg gtcgcttccg
     9601 cgaagccggc cgcggcgacc gggccagtgt cgggcacccc gatcactaga tccgcttcga
     9661 ccggcgcttc gtcggcgagg cgccggccga actcgcgacg cacctcgtgc acgccgtgct
     9721 cgtagagcag gctgtccgga cgcgcgaggt agacatactc gaagatgcac agcttgggcg
     9781 tgggcttggc gtagatccag ctgcgcaggc cgcggtcgtc gacggcgacg atctcgccgg
     9841 gctcgacgtc gcgcacgtac gcggcgccga cgatgtcgag cgcgcaggtc tcggaggcga
     9901 gcacccaccc gccgttggcg agccggccga tcgacagcgg ccggatcccg tacgagtcgc
     9961 gcacgccgta cacggttcgt tcgtccatga ccacgaagct gaaggcgccg gcgagttcgg
    10021 gcatgagcga catcagcgcg tcctcgagcg gcatctcgcc ggtgcgctcc gcgagcagct
    10081 ccgcgacgat gtcggagtcg gtcgatgcct tcggcccgtt ggtcttgcga gtgaccttgg
    10141 ccgcgagcgc ccgggtgttg gtcaggttgc cgttgtggcc cagcgccagg ccgccgccgc
    10201 tcccggtcgg cttgaaggtg ggctgggcgt tctcccaccg ggtggagccc gtcgtggagt
    10261 agcgcgtgtg gccgatcgcg aggggaccgg acagggtcgc gagcgtcgcc tcggaaaaga
    10321 cctgggcaac caggcccatg tccttgtaga cgatgatgcc gttaccgtcg gaaacggcga
    10381 tgccggcgga ttcctggccg cgatgctgga gcgcgtagag gccgaagaag gtgagcttgg
    10441 ctacctcttc acccggcgcc cagacgccga agaggccaca ttcttccttt ggagagtcgg
    10501 cgcccattcg tcctccagtc tatcaggcga ggaggtccgg aagcgtgcgc ttccacgcac
    10561 ttcggagcgc cgaaaccggc agatcgaaat ctccgcaggc gagcgattcg ccgccgaccg
    10621 tgccgatggc cgtcgcgggc acgccggcgg aggcggccgc aaccaaaacg gcttccaggc
    10681 cgctcgcggg acacgagacc agcgcgcggc tcgccgactc gctgaaccac agcacgtggg
    10741 gcgccagagc cgattcgagg ctcacgcggg cgccgatctc gccggccatg cacgattccg
    10801 cgagcgtgac ggccaggccg ccttcgctgc agtcgtgcgc gctgaccagc aaccggcctt
    10861 cggcgagcgt cgccagcagg tcgcacagcg ccttttccat cgtcagatcc agcttcggcg
    10921 gatgcccgcc gagatgccgg tgcacggccc aggcgtactc gctgccgccg agctcgtcga
    10981 aggtttcgcc caacagcacg atcgcatcgc cttcgcctcc gaacgcgatg ccggtgtgga
    11041 tcgtggcgtc ttcgaggatg ccgagcatgc cgacgaccgg cgtcggatgg atcgaggtcc
    11101 cgtcggtctc gttgtagaag ctgacgttgc cgccggtgac gggcgtctcg agcgcgaggc
    11161 aggcttcact catgccctgc gtcgcctcgg cgaactgcca catcacgtcg gggcgctcgg
    11221 ggttgccgaa gttcaggcag ttgctgatcg cgagcagccg cgcgccggtg caggcgacgt
    11281 tgcgggcgga ttcggccacg gcgtgcatgc cgccgaggcg cgggtcgagc tgtccgtagc
    11341 ggccgttgcc gtcggtcgag atgaccagcg cgcgcggcgt gccttcgacg cgcacgacgg
    11401 ccgcatcgcc gccgggacct tgcagcgtgt tgtgctgcac gagatagtcg tactgctccc
    11461 atgcccacga cttgtcgcac acgttcggtg cgcccagcac ggtcagcagc gcttcaccga
    11521 tgtttttcgg cgcgggcagc ccggccgggt cgtccgcgtg cagcgcgtcg atccagtccg
    11581 gccgctccat cggacggttg taggtcgggc cgtcgtcggc gagcgagcgt gccgggatgt
    11641 cggcgacgac ctcgccgagc tgcttgatga cgagccggtc gccggcggtg accttgccga
    11701 cgatcatcgc gtccaccccc cagcgctcgc ataccgcaac ggcacgctcg acgtcctcgg
    11761 gcttgacgat ggcgaacatg cgctcctgcg attcggaggt gaggatctcg aacggctcca
    11821 tgtcgggctc gcgcagcagc acggcgtcga ggtcgatctc catgccgacc tcgccgcggc
    11881 tggcggtctc gctggtcgcg cagcaaaatc ccgcgccgcc gagatcctgc acgccgacga
    11941 tcaggccttg ctcgagcagt tcgagcgttg cctcgatgac gaccttctcg ctgaacgggt
    12001 cgccgacctg aacgctggga cgcttcgctt cggcttcggc gtcgaagggc cgcgatgcga
    12061 gcacgctgac gccaccgatt ccgtcgcggc cggtcttggc gccgtatagg atcgcgacgt
    12121 tgccttcgcc ttgcgcgcgg gcacgcgcga caaggtcctt gcgcatgatg ccgatgcaca
    12181 tcacgttcac cagcgggttg ccggcgtagc agggatcgaa cttgatctcg ccgccgacgg
    12241 tcgggatgcc gatggagttg ccgtactggc cgatgccttg cacgacgccg cccagcagat
    12301 gccgctgacg ccattcggcc gggtcaccga aacgcagcgg atccagcagc gcgaccggac
    12361 gcgcgccggt ggcgaggatg tcgcgcacga tgccgccgac gccggtcgcg gcgccctgca
    12421 ccggctcgac cgcgctggga tggttgtggc tctccagctt gaagaccgcc accagaccgt
    12481 cgccgatgtc cacgacgccg gcgttctcgc ccggtcccat cacgatgcgc ggaccggtcg
    12541 tgggcagttt cttcagatgc accttcgacg acttgtacga gcagtgctcg gaccacatcg
    12601 ccgcgtacat ggcgagctcg gccggcgacg ggtcgcggcc gagcgtctcg acgatcttgt
    12661 tcagctcgcc ctcggtcagg ccgagcgcgg acgcgcgttc gatcagcgtc gcgctcaacg
    12721 ggccaccgcc ttcaggatcg agccaagaat gatcgcaccg tcggcgcttc gaatcccgac
    12781 ggtgtcatcg accgcgtgct cgggatgagg catcatgccg agcacgttgc cggcctcgtt
    12841 cgtgacaccg gcgatgccgg ccaccgatcc gttcgggttc tcaccgtcgt atcgcagcgc
    12901 gacctgaccg ttggcctcca tgcgcgcgat cgtgccggcg tcggcgacgt agcggccttc
    12961 gccgtgcttg atcgggatgc gaagcgtatc gccggcgttc gtgccactca ggtgcggtga
    13021 ccggttcgat tcgacccgca gcgtcaccgg aacgcagcgg tacttcaggc cctcgttgcg
    13081 caccaacgcg ccgggcaaca gaccggcctc gcacagaacc tggaagccgt tgcagatgcc
    13141 gagcacgatg ccgccgtcac gcgcgaacgc cgcgacctgc tccatcaccg gcgcgaagcg
    13201 ggcgatcgcg ccgcagcgca ggtagtcgcc gtaggagaac ccgccgggca cgatgatcgc
    13261 gtcggcgccg cgcaggtcgt ggtcgccgtg ccagatcagc tcggcctgcg cgccgagctt
    13321 ctcgaacgcc cagaccgcgt cgcggtcgtc gagcgaaccg gggaactgga tgactccgat
    13381 cttcacacgg cctcctcagg cacctcgtgg aaatcctgcg gcgacgagct gctggatcgc
    13441 acgctcacga ccgaattcga ccagccgctg cgcgtccgtg caaccgcgat cccacgcctc
    13501 gatgtcgatc agcaagcggc atgcttgcag ctcggcctcg cgggcccgga actccagctc
    13561 ggccgtcaac ggctcgtccg ggtgcagcag atgaaatcct tcgtgcacca gcagtggacc
    13621 gatcagcgcg gccgacacat cccgctgcgc gaaccggatg ttgatcagga tgcggacggg
    13681 atcggcggac gacagcgtgg agaagtcgcc ggaccgctgg aacacggcga atcgcagccg
    13741 ggcctcggac agcgcccgga tcgaccgcgc cacgtcggac tcgcccgcgg catgcggaca
    13801 gagccgctgc agcacaccgc cgagccggac gtgcgcgggc tcgccgcctt cgatcacgac
    13861 gccctcgacc gctcggcacg gcgcacgagt gacggggaga tcctcgggaa tcggggccag
    13921 cacgatctgg ccgacgtcgt gcgagagcgt tttcgacggc gggctgcgcc gctcggcgat
    13981 cgcgatctcc gtcgccggtg ggttgatcag catgacgacg gtcagcacga tgccggtgag
    14041 cgcgaccacc gcgaaggcgg ccagcaaggc cgggtgcatc gctttccgaa acggaagatc
    14101 gatgcccttc ggcttggccg ccttcttggc cgcacgagcc gctcttcgct cctgccgtcg
    14161 ctgccggcgg taggagcgat agtcctgctc ggacgcggac cgttcctcgt cggtggtcgt
    14221 gctcattctc cggattcgtc gatccgatac gtgaagtcct cgatcacggg attcgacagg
    14281 agcttctcgg ccatgtcgcg gacctgggcg tcgactgcct cgcggctctc ggcttcgatg
    14341 tcgaacacga tgtgcttgcc gatccggacg ccgctgacgc ccttgaagcc gagcgacggc
    14401 aggtttcgct cgacggtctg gccctgcggg tccaggatgc cgtccttgag catcacgtcg
    14461 agcaggacgc gaaccttcac ttgggctcca gctccgcggt cgcgaggtag cggtcgaacg
    14521 acgcgccgct gacgacctcg aacgcctcgg cgtacttggc gcgcgtgctg gcgaccacgt
    14581 cgtccggaag ctccgggccg ggcggggtgt ggtcccagcc gctcgcgtcg agccagtcac
    14641 gcacgtactg cttgtcgaac gacggcgggc ttttgccggg ctcgtactgg tcggccggcc
    14701 agaagcggct ggagtcgggc gtgagcactt cgtcgatcag gatcagctcg ccgctcggga
    14761 gcgtgccgaa ctcgaacttg gtgtcggcga tgataatgcc cttcgagcgc gcgtagtcgg
    14821 cggcttcgct gtagatcgtc agcgtcgcgt cgcgcagctt ctccatcgtc tctttgccgg
    14881 cgatctcgca ggcgcgttcg aacgagatgt tctcgtcgtg ctcgccggcc tcggccttcg
    14941 tggcgggcgt aaagatcggc tcgggcagct tgtcggactc ttgcagaccg gcgggaagct
    15001 tgatgccgca cacggtctgg ttcgccttgt attccttcca gccgctaccc gacaggtagc
    15061 cgcgggcgac gcactcgatc ggcacaacgg tcgtttcctt gacgagcatc gcgcggccgc
    15121 gcaacgcgtc agcgtgctgc ttggcctccg gcgggaagtc ctcgggtgcg gttgcgatca
    15181 ggtggttgcc gatgatgtca tcgaagcgac cgaaccagaa ctttgacagc cccgtgagca
    15241 cgctgccctt atcggggatc ggcgtgggaa gcaccacgtc gtaggcgctg atgcggtcgg
    15301 aggcgaccaa gaggatgcct tccgaggtgc ggtacagctc acgaaccttg cccttcctga
    15361 tggattccag accggcgagc gtcattcgat tccctccagg cgcttgaaaa cgacatcgac
    15421 atgagtcaag taacgggatg gatccatgat tcgtgcgaga tcgtcgcgac tcgcgaccgc
    15481 ggtgacctcg ggtacgtcca tgagcgtgtc ggcgaacggc gtgccggtct cccaggtgcg
    15541 catcgccgcg gtctggaccg ccgcgtacgc gtcttcgcgg gtcatgccgg cctcgacgag
    15601 cgcgagcagc atcgagccgg agtaaacgag gccgccggtg gcgtccaggt tctgccgcat
    15661 gcgctcgacg tccacggtca tgccttcgat cacgtcggtg gtgagcttga ggatgtagtc
    15721 gagcgcgatc gtcgcgtcgg gcatcatcac gcgctcgacg ctcgagtggc tgatgtcgcg
    15781 ctcgtgccac agggccatgt tctcgatcgc gggcacgacc gcggcacgca ccacgcgagc
    15841 cagaccgctg acacgctcgc agatgatcgg attacgcttg tgcggcatcg ccgacgagcc
    15901 cttctgtccc ttgcggaacg gctcctcggc ttcgcggacc tcggttcgct gcaggtgacg
    15961 gatctcggtg gcgatcttgt cgagcgttcc ggccgtgatc gcgcaggcgg ccatcagctc
    16021 ggcgtgccgg tcgcgctgga tcacctgcgt cgcggcctct gcgctcgaca ggcccagctc
    16081 ggcgcagacg tagtcctgca cgaacggatc gatcgaggcg taggtgccga cggcgccggc
    16141 gatcttgccg accgacacga tgtcgcgggc acggcgaagc cgttcccggt cgcgtgcgat
    16201 ctcgaacgcc cacaccgcca tcttcagccc gaacgtcgtc ggctcggcgt gcacgccgtg
    16261 cgagcggccg acgcagacgg catccttgaa ctcgatcgcg cggcgcttga ccgcctcgag
    16321 caggtggtcg gtcttggcga gcaggatgtc gcaggcgtcg cgcatctgca gcgcgagcgc
    16381 cgtgtcgagc agatccgacg acgtcatgcc gtagtggatc cagcgcgatg cggggccgat
    16441 cttttcgccc atcgcggtca ggaacgcgat cacgtcgtgg ttggtcgtcg cctcgatctc
    16501 ctcgacgcgt acgggatcca cctcggcggc gttgtcccgg atcgtcgtgg cgtcctcggc
    16561 cgggacgacg cccagcttgg cccacgcgtc caccgcgagc agctcgacct gcagccagcg
    16621 gcggtacttg gagcgttcct cccagaccgc agccatctcg ggcaggctgt agcgagggat
    16681 cacaggcgca gtccctccgc gagttgctgc ttgtactcgt ggagcttctt gcgaaggtcg
    16741 tcgtcgccgg cagccaggat ctgcaccgcg agcaggcccg cgttcgacgc cgcgtccacc
    16801 gcgaccgtgc cgaccggcac gcccgagggc atctgcacga ccgaaagcag cgcgtccatg
    16861 ccgtccatga tcccgccaga gagcggaacg ccgatcacgg gaagcggcgt aagcgaggcg
    16921 accacgccgg gaaggtgcgc ggcacggccg gcaccggcga tcatcacgcg caggccgcgt
    16981 gactccgccg aggtgacgta ctcgtgcaca cgcgccggct ggcggtgcgc gctcatggct
    17041 tccatttcga actcgattcc gaatttctcg agcacgacgc cggccggagc catcttgtcg
    17101 cggtccgacg ggctgccgaa gacgattcct acttgcacgc tcatagcttt ctctctcccc
    17161 ccatcgcacg ggatccgata tcgcgacggt attgcattcc gttgaacgag atcttggatg
    17221 cggcttcgta ggcgcgatcg cgcgcggacg cgacgtcgtc gcccatgccg gtgacgccca
    17281 gcacacgccc accgttggtg acgacgcgtc caccgtcgcg ggccgtgccc gcgtggtaga
    17341 cggtgacgtc cttcacctgc gcggcggcgt cgagcccgtc gatcggcgtg cccgtgtcgc
    17401 tgcgcgccgg atagccttcg cccgccagca ctacgcagac cgcggtgcgg tccgacaggc
    17461 gggcggcggc gcggtcgagg gagccgtcgg cgcaggcga
//
LOCUS       DATLFO010000185         6293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_47653, whole genome shotgun sequence.
ACCESSION   DATLFO010000185 DATLFO010000000
VERSION     DATLFO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47653"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..261
                     /locus_tag="VM841_07425"
     CDS             88..261
                     /locus_tag="VM841_07425"
                     /inference="COORDINATES: protein motif:HMM:NF027645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HVL90048.1"
                     /translation="MRRKGRFSVEPMTPKEAALQVDASGEEFLPFVNAETDQISVVYR
                     MSDGGFGLLEPAE"
     gene            308..1009
                     /locus_tag="VM841_07430"
     CDS             308..1009
                     /locus_tag="VM841_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90049.1"
                     /translation="MADETTVTEQSEREDQIRVLIVDDHALFRRGLQMVLEQEEDISV
                     VGEAGDGTEAVDKAVETMPDVVLMDVRMPRRGGIEATAKIKESLPSTKILMLTISDEE
                     ADLYDAIKAGANGYLLKEISIDEVAAAIRSVQTGQSLISPSMASKLLGEFAQMVRKAE
                     EKPAVPQPRLTDREMEVLQLVAQGRNNRDIAKELFISENTVKNHIRNILEKLHLHSRM
                     EAVVYAVREKLLEIK"
     gene            complement(1016..2473)
                     /locus_tag="VM841_07435"
     CDS             complement(1016..2473)
                     /locus_tag="VM841_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90050.1"
                     /translation="MRLRAVALLLALAGLPALAPLAPASAAGITVGGLSEPGTSGAIR
                     VLSIAADKRGVYRPQTGDPNDRSQDPSRTVGDVPSIGDGGPGCAPVISTDCFRSLAVN
                     EASVNASATDGHAHYHAGDTSIVVTEVKHFRAGDTIMIGSPHCNYARLGLTCERYLPG
                     FGMVREQPTGEIVAIEGGDVGPSVHGPGVLRLSHPLAFDHRPGEQVVKLPTVYPGHDL
                     RIDVPNAALLGSHPPPLHVRATFIPPQGSRIPWDAGFRRPLARATSKTVFEQIVHGPD
                     GTTSFWMPREITYTTTAGRTFSIDTEDSWYTIEIVARDGGGRVVQDGVARFKTNPLAI
                     PFMTTDPQRVFPGNAVQITGWVRDGFSPATMSRRVEDIDVNVVVTKPRGSQSFKASSC
                     YDSEPGPDDICGGDPFGATPNQHGNFNVRMGGTRCNEENLGICMRGNIYDRMGDTQDR
                     GTYTVTATVRGVTPAVRAVTTFRVAWPDPLDGVVP"
     gene            complement(2470..3942)
                     /locus_tag="VM841_07440"
     CDS             complement(2470..3942)
                     /locus_tag="VM841_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90051.1"
                     /translation="MPGHDARPRRAAGRAVLVACALLASLALPASSPAFAERDLTPPG
                     EIGAVRIRPVAVRPDGSMGLIHGDPKFTFTDPNRTVGDVEPPWPAGAGVPCTDPSMPD
                     DCARTLASNEPDVNPSAGDGHTHTHTGDRWIIVNEIHGFARGDTIMIGSPSCDFGALG
                     FTCRKYRPGFGIQSAQPNGEIAYVTGGDAGKGLRGPGLLRLRDGLLHSHPEGTRIVKL
                     PTAYTGQQLHVVVPHEALLGSGLEPVTVTATLIPPAASRDPAGDRLRLPVDHSHLDRY
                     ERIVSSPAGDAIFYVPRSMTGVATIGRGFDIATEDTWYAVEIVSRDATGRPVAYGIAR
                     FKLNPVMMRDLRIDRTRIPPTYPVAIEGSLRDASSPEAANRPVEDATVEVMVTKPDGT
                     KKGFYANACTDNDPGPRTVCGGDEVGYQPRHYGAFKIHVGGMRGFQARGQFQFCRPCM
                     ERNLNSAMDTAQQGTYRAIVSVYHVSPFVGGQVQWEVRVL"
     gene            complement(4207..5790)
                     /locus_tag="VM841_07445"
     CDS             complement(4207..5790)
                     /locus_tag="VM841_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90052.1"
                     /translation="MKFRFASVKRPLLALTALAAMIAVTGGVVPAAAVTHNNLSFPGT
                     TGKIRVHSIFQDNLGATKQQVGDTKDPTTNPNRTVGDVPVNVSNQFNTACTPAATDCL
                     RTLAVNDPAVNISAADGHAHYHTGDTKIIVNQVAGFRRGDSVMIGSPYCNHAFLGLTC
                     KKFVPGFGIVDEQPTGEVVQVVGGDIAPGNHGVGVLELGGAGLTNDVCPTPAPLAPCA
                     HLEGTQVYKLPTLYQGQQMRVDIPHADLLDCDILLTTCNPNDVTVTADFLPPQGSHDP
                     AFPRRPLALNRDNPNGPFAAEVPATCGPTPCGAGYFSFFIPKEITGTATIGNTFNVNK
                     TDTWYTVEVKAIDSTDGHLVADGIVRFKLTPAALVELKAENPTRPGVTKFFPTESVEI
                     TGAVRDNSSAAALYRPVEDVIVDVAVTKPNGSTRRYQVTSCVNTAPAGATDSCGGNVF
                     RSQPNGHGKFLVTFGGPSGLHLGSNPTCNPSSITTCLSRVVNGMLDTSDTGTYDVLAT
                     LRGYRPTVSASTTFDVAIF"
BASE COUNT          980 a         2053 c         2226 g         1034 t
ORIGIN      
        1 atccgaaagc catcttcccg accgaaagga cggaccaccg agggcgatcg ggtagaatcg
       61 ccgaagaaca cgcgggtcgc cccgcgggtg cgccgaaagg gccggttctc ggtagagccg
      121 atgaccccga aggaggccgc cttgcaggtc gacgcgtcgg gagaggaatt cctcccgttc
      181 gtgaatgcgg agaccgatca aatcagtgtg gtctaccgga tgagcgacgg aggattcggg
      241 ctcctcgagc cggccgagta gtcaggaatg cggagccgga acggaacggc cgagtgaggt
      301 gacacgggtg gcggacgaga cgacggttac cgagcagtcc gagcgcgagg accagatccg
      361 ggttctcatc gtggatgacc atgcgctgtt ccggcgtggc cttcagatgg tgctggagca
      421 agaggaagac atctcggtcg tcggcgaggc cggcgacggc accgaagcgg tcgacaaggc
      481 cgtcgaaacc atgcccgacg tcgtgctcat ggacgtgcgc atgccgcgcc ggggcggcat
      541 cgaagcgacc gccaagatca aggagagcct cccctccacc aaaatcctga tgctcacgat
      601 ctcggacgag gaagccgacc tctacgatgc gatcaaggcc ggggcgaacg gctatctgct
      661 caaggagatc tccatcgacg aggtcgcggc cgcgatccgc agcgtgcaga ccggccagtc
      721 gctgatctcg ccgtcgatgg cctcgaagct gctcggcgag ttcgcccaga tggtccgtaa
      781 ggccgaagag aagcccgccg tcccgcagcc gcgcctcacc gaccgcgaga tggaggttct
      841 gcagctcgtc gcgcaaggcc gcaacaaccg cgacatcgcc aaagagctct tcatctccga
      901 aaacaccgtg aagaaccaca tccgcaacat cctcgagaag ctccacctgc attcgcgcat
      961 ggaagcggtc gtctacgccg tgcgcgagaa gctgctcgag atcaagtagc gccggctacg
     1021 gcacgacgcc gtcgagcgga tcgggccagg ccacccggaa cgtcgtcacc gcacgcaccg
     1081 ccggcgtcac gccgcgcacc gtcgccgtca cggtgtaggt gccccgatcc tgcgtgtcgc
     1141 ccatccggtc gtagatgttt ccccgcatgc agatcccgag attctcctcg ttgcagcgcg
     1201 tcccgcccat gcgcacgttg aagtttccgt gctggttggg ggtcgcgccg aacggatccc
     1261 cgccgcagat gtcgtccggg ccgggctcgg agtcgtagca cgacgacgcc ttgaagctct
     1321 gcgacccgcg tggcttggtg accacgacgt tgacgtcaat atcctcgacg cggcgcgaca
     1381 tcgtcgcggg gctgaacccg tcgcgaaccc agcccgtgat ctgcacggcg ttgccgggga
     1441 acacgcgttg cgggtcggtc gtcatgaacg gaatcgccag cgggttggtc ttgaaccggg
     1501 cgacgccgtc ctggacgacg cgaccgccgc cgtcccgggc gacgatctcg atcgtgtacc
     1561 aggagtcctc ggtgtcgatc gagaacgtgc ggcccgccgt cgtggtgtag gtgatctcac
     1621 gcggcatcca gaacgaggtg gtcccgtccg gcccgtggac gatctgctcg aagacggtct
     1681 tgctcgtggc gcgggccagc ggcctgcgga agcccgcgtc ccacgggatc cggcttccct
     1741 gcggcggaat gaacgtggcc cgcacgtgca gcggtggcgg gtgcgagccg agcagcgccg
     1801 cgttcggcac gtcgatccgc aggtcgtgtc ccgggtacac cgtgggaagc ttgacgacct
     1861 gctcgccggg ccggtgatcg aaggccagcg gatgcgacag ccgcaggacg ccgggaccgt
     1921 gaaccgacgg gccgacgtcg ccgccctcga tcgccacgat ctcgccggtc ggctgctcgc
     1981 gcaccatgcc gaacccgggc aggtagcgct cgcaggtcaa tcccagccgc gcgtagttgc
     2041 agtgcggcga gccgatcatg atggtgtctc ccgcccggaa gtgtttgacc tcggtcacca
     2101 cgatggatgt gtcgcccgcg tggtagtgcg cgtgcccgtc ggtggccgac gcgttgaccg
     2161 acgcttcgtt gacggcgagc gagcgaaagc aatccgtcga gatgaccggc gcgcatccgg
     2221 ggccgccgtc gccgatggac ggcacgtcgc cgacggtgcg agacgggtcc tgggaccgat
     2281 cgttcgggtc gccggtctgc ggccggtaga cgccgcgttt gtcggccgcg atcgaaagga
     2341 ccctgatcgc gccgctggtg ccgggctccg acaggccccc gaccgtgatc ccggcggcgg
     2401 acgccggtgc gagcggggcg agcgcgggca gtcccgcgag cgcgagcagc aaagccacgg
     2461 cgcggagcct cacagcactc gcacctccca ctggacctgg ccgccgacga acgggctcac
     2521 gtggtagacg gacacgatcg cgcggtacgt gccctgttgc gcggtgtcca tcgccgagtt
     2581 caggttgcgc tccatgcagg gccggcagaa ctgaaactgg ccccgggcct ggaatccgcg
     2641 catgccgccg acgtggatct tgaacgcgcc gtagtggcgc ggctgatagc cgacctcgtc
     2701 gccgccgcac acggtgcgcg gcccgggatc gttgtcggtg cacgcgttcg cgtagaagcc
     2761 cttcttcgtg ccgtcgggct tggtgaccat cacttcgacg gtcgcgtctt cgaccggccg
     2821 gttcgcggcc tcgggcgaag acgcgtcccg caacgatcct tcgatcgcga cgggataggt
     2881 cggcgggatc cgggttcgat cgatccgcag gtcccgcatc atcaccggat tcagcttgaa
     2941 tcgcgcgatg ccgtacgcaa ccggccgccc ggtcgcgtca cgtgagacga tctcgacggc
     3001 gtaccaggtg tcctcggtgg cgatgtcgaa gccgcggccg atggtcgcca cgcccgtcat
     3061 ggaccggggc acgtagaaga tcgcatcgcc cgccggcgag gacacgatcc gctcgtagcg
     3121 atcgagatgc gaatggtcca ccggcagacg gagccggtcg ccggccgggt cgcgcgaggc
     3181 cgccggcggg atgagggtag cggtcacggt gacgggctcg aggccgctgc cgagcaatgc
     3241 ctcgtgcggc acgaccacgt gcagctgctg gccggtgtag gccgtcggaa gcttcacgat
     3301 gcgtgtgcct tcgggatgcg agtgcagcag cccgtcacgc agacgcagaa ggccgggccc
     3361 gcgcagtccc ttgccggcgt cgcccccggt cacgtaggcg atctcgccgt tcggttgcgc
     3421 gctctggatg ccgaaacccg gccggtactt gcggcacgtg aagccgaggg ccccgaagtc
     3481 gcacgagggc gatccgatca tgatcgtgtc gccccgggcg aagccgtgga tctcgttgac
     3541 gatgatccac cggtcgcccg tgtgcgtgtg cgtgtgcccg tcgcccgcgg acgggttcac
     3601 gtccggctcg ttgctcgcga gcgtgcgagc gcagtcgtcg ggcatcgacg ggtcggtgca
     3661 cggcacgccg gcgccggccg gccacggcgg ctccacgtcg cccaccgtcc ggttgggatc
     3721 ggtgaaggtg aacttggggt cgccgtggat gagtcccatc gatccgtccg ggcggacggc
     3781 gaccggccgg atacgcaccg cgccgatctc gcccggcggc gtgaggtctc gctcggcgaa
     3841 ggccggcgag gaggcgggga gcgcgagcga ggcgagcagc gcgcacgcga ccaggacggc
     3901 cctgccggcc gcccgccgtg gtcgtgcgtc gtgtcctggc atcgttggtc gtggtcgcgc
     3961 ttctggccgt ggtcgcactt ctggttgcgt ccctgagccc ccgccccttc agttcgaggc
     4021 gagcgatggt cctccccgct gcgggatgcc cggtcgtgcc gcccgggccg cagtgccccg
     4081 gctcgtccga tccgtccccg atgcccgatg caccgggtgg ctgcggccgg tttgggacgt
     4141 caaaagacgc caaaaagagg agagaccccc gaaggggtct ctccgcggct cggagctatc
     4201 agtttgttag aagatggcga cgtcgaaggt ggtggaggcg ctgaccgtgg gacggtaccc
     4261 gcggagcgtc gccagcacgt cgtacgtgcc cgtgtcggac gtgtccagca tcccgttcac
     4321 cacgcggctg aggcaggtgg tgatgctgct cggattgcag gtcgggttgc tgcccaggtg
     4381 caggccgctc ggaccgccga aggtgaccag gaacttgccg tggccgttcg gctggctgcg
     4441 gaacacgttg ccgccgcagg agtccgtcgc gccggccggg gccgtgttca cgcacgaggt
     4501 cacctggtag cgacgcgtgc tgccgttcgg cttggtgacc gccacgtcca cgatgacgtc
     4561 ctcgaccggg cggtacaggg ccgcggccga cgagttgtcg cggacagcgc ccgtgatctc
     4621 caccgactcg gtcgggaaga acttggtgac gcccggacgg gtcgggttct cggccttcag
     4681 ctcgaccagc gcggccggcg tgagcttgaa gcggacgata ccgtcggcga cgaggtgacc
     4741 gtcggtcgag tcgatcgcct tgacctcaac cgtgtaccac gtgtcggtct tgttgacgtt
     4801 gaacgtgttg ccgatcgtcg cggtaccggt gatctccttc gggatgaaga aggagaagta
     4861 gccggcgccg cacggcgtcg ggccgcacgt tgcgggaacc tcggccgcga acgggccgtt
     4921 cgggttgtca cggttgagcg ccagtggacg acgcgggaac gccgggtcgt gcgaaccctg
     4981 cggcgggagg aagtccgccg tcacggtgac gtcgttcggg ttgcacgtcg tcagcaggat
     5041 gtcgcagtcg agcaggtcgg cgtggggaat gtccacgcgc atctgctggc cctggtagag
     5101 ggtcggcagc ttgtagacct gcgtgccttc caggtgtgcg cacggagcca gcggagccgg
     5161 cgttgggcac acgtcgttgg tcaggccggc gccgcccagc tcgagcacgc cgacgccgtg
     5221 gttgccgggg gcgatgtcgc cgccgacgac ctggacgacc tcaccggtgg gctgctcgtc
     5281 cacgatgccg aagccgggga cgaacttctt gcaggtgaga ccgaggaacg cgtggttgca
     5341 gtaaggcgag ccgatcatca cgctgtcgcc ccgacggaag ccggcgacct ggttgacgat
     5401 gatcttggtg tcacccgtgt ggtagtgcgc gtgaccgtcc gcggctgaaa tgttgacggc
     5461 cgggtcgttc acggcgagcg tccggaggca gtcggtcgca gccggggtgc acgccgtgtt
     5521 gaactggttg ctcacgttga ccgggacgtc gccgaccgtg cggttcgggt tggtcgtcgg
     5581 gtccttggtg tcaccgacct gctgcttggt ggcacccagg ttgtcctgga agatcgagtg
     5641 cacgcgaatc ttgcccgtcg ttccggggaa cgacaggttg ttgtgagtga cggccgcggc
     5701 cggcacaaca ccgccggtga cggcgatcat cgccgccaac gctgtgagcg ccaggagtgg
     5761 ccgcttcaca gacgcgaagc gaaacttcat ttgaatccct ccctttcctg gcgctccgag
     5821 ccttggttga ggaacgccat tggaacttct cccctgactc ccgcctgctg gcggaagcct
     5881 ttgccctgtg tcgcggatgt ttcgaccggc ctgccttctc ctcctgctct gttggcaggc
     5941 gagcatgtag ggctttactc attggtggag aatttggctt aattcttcag ggcaatccgg
     6001 gggagtctgt gaaaagcggg gtgagaatgt tccctttcgg ctagcaactt gaacaattcg
     6061 aaccttccgg taccccgccg gttcgctgcc cgacctcaca ccccccgatc cgcccgatcg
     6121 cgcccccggc gccggtccat ccctcggccc cggcgcgcat atctgcaacc cgcttgcacg
     6181 tctgcccgat cgcacgccga atggccggtg tcgatttcag atctgcaagc gggttgcaga
     6241 tatgcgcgcc ggggccgagg gatggaccgg cgccgggggc gcgatcgggc gga
//
LOCUS       DATLFO010000186         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_34636, whole genome shotgun sequence.
ACCESSION   DATLFO010000186 DATLFO010000000
VERSION     DATLFO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34636"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..378
                     /locus_tag="VM841_07450"
                     /pseudo
     CDS             91..378
                     /locus_tag="VM841_07450"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
     gene            complement(527..1072)
                     /locus_tag="VM841_07455"
     CDS             complement(527..1072)
                     /locus_tag="VM841_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90053.1"
                     /translation="MGRRFVRKLFLSVTSMALALTLVGGAYALAGDPTAEEETPTVEQ
                     TQTGDPQPLEETEAPEPLEQETEPLEEEDEDGVRGGSVERIHEGCEGVEGLEGNWTHG
                     DYVKAVGIQSGNEAKKAAAKSDCGKPNTGKRARGDDAMPDKAEKKAEKKAGTPGGTPG
                     GTPGSDSGGDDEDGSGEDEPD"
     gene            complement(1255..2583)
                     /locus_tag="VM841_07460"
     CDS             complement(1255..2583)
                     /locus_tag="VM841_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90054.1"
                     /translation="MKRVKRTFAVAVAMAALAASIFVPNSASAGGVTINDNPTANPPA
                     WDTALGGKSVNAAGTVSNCDTLNAVLSPNKNFFAYNNGPPVQNGAPNPRGCLGEADIQ
                     SFSLDSTTVPGKLTWKMTTRQLPRPGTPPSTLAAAGGGEFGYYVFFQNPRRQNARQII
                     GDAPDCTRFSHAGPYLTGNNEYFFLYWDVHLGPTGTYQVRNHGWGRWDPVGNILYQTR
                     TSADAPQSNVLSRFCSGTPATHQTVNFPGTGVNLSYSGNTMTMSVPFNYCWINLSNIV
                     NCYEIAAPGDQIVGVQAATHGGVFIGTPDLQSSGGPGPTGAGQNLLVDWAPYAAYDYG
                     TFTPQTERLPGPNCPNFATAAEYFTSNESPVTPAYVTIGKGPGGADNDYQVNPNYGLN
                     RYDASGETGNKNQSPALPTGPQNHHGATTCDIFIPTAARFIAGAGGSFTV"
     gene            complement(2614..3270)
                     /locus_tag="VM841_07465"
     CDS             complement(2614..3270)
                     /locus_tag="VM841_07465"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL90055.1"
                     /translation="MRRHSALVVLAGVLVGMLGMAPASASSSVDILSPTGGYVPGSFT
                     VLVAADDDDGVASVELLVDGISAGSVISEPFEFALTGSDDTTIQLVARMTDSLDNVTN
                     SAPVTVTVDAVAPSLSITTPQGSTLLNLPTVNEFIGGDGVEGTSSDDGSGVVTIAVNL
                     THTTLNLSESGHASIADDGSWEYVPAFALVPGEYLVDATPVDAADNVGATATVVVTVV
                     "
     gene            complement(3407..>3775)
                     /locus_tag="VM841_07470"
     CDS             complement(3407..>3775)
                     /locus_tag="VM841_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90056.1"
                     /translation="VPDFVKAEVPEDEAWGSACFVPRVGSACRVPFEGVAYLSTQDKA
                     QIALVAYENKSTTPAAIRLLPAEKGPNRLCFESGCPHGGAWLSYVPSPGTKTVTFFVE
                     LRATGGAVLAKSFPKAYRIH"
BASE COUNT          576 a         1246 c         1287 g          666 t
ORIGIN      
        1 cggccaccgg catgtccagc gtcggcgagc cctcgaacca gcgaaccgag ttgatcacga
       61 tcgagccgcc cgcatgacac gaccacttgc cgcgcgtgtc ggcgggccgg cacgcgcggc
      121 aagtggtcgt gtcatgcggg cggctcgatc gtgatcaact cggttcgctg gttcgagggc
      181 tcgccgacgc tggacatgcc ggtggccgac tatcgcgcgc tggtcatcaa ccacgagacc
      241 ggccacgcgc tcgggttcgg gcacagcggc tgcggcggcc gcggcacgct cgcccccgtg
      301 atgatgcagc agtccaaggg cttgtccggc tgtctgcaga atccgtggcc gaccgcgcgc
      361 gagctcgcgc ggctctagcg tctctgcagc agcttcagct gctgcggctg cgaacgccgt
      421 gacgctttcg tcgcgggcgc gcggggcctc gcgggccgac aacgccgaag ggacggcctc
      481 ttcgagcggc cgtcccttcg cggggaggaa actgcggagc tagtcactaa tccggctcgt
      541 cttcacccga gccgtcttca tcgtccccac cggagtcgct tcccggcgtg ccacccggcg
      601 tgccgcccgg cgtgccggcc ttcttctcgg ccttcttttc ggccttgtcg ggcatcgcgt
      661 cgtcgccacg cgcgcgcttg cctgtgttcg gcttgccgca atcgctcttg gcggcggcct
      721 tcttggcttc gttgccgctc tggatcccca cggccttcac gtagtcgccg tgcgtccagt
      781 tgccttcgag accttcgaca ccctcgcagc cttcgtggat gcgctcaacg gagccgccac
      841 gcactccgtc ctcgtcctct tcttcgaggg gctcggtctc ctgttcgagg ggctccggcg
      901 cttcggtctc ctcgaggggt tgaggatcgc cggtctgggt ctgctcgacc gtcggcgtct
      961 cttcctcggc ggtgggatct ccggcgagcg cgtacgcgcc accgaccagc gtcaacgcca
     1021 gcgccatcga cgtgacggac agaaacagct ttctgacgaa cctgcgaccc atcgttcatc
     1081 ctctcctcgt gcggcgttcg gacaaggaag cgacggacta gtccgtgcgg gtacggcgga
     1141 caatcgtgcc cgcacagcac gcaccgggcc gaagcggact ctgcggctcg aaaaaatcag
     1201 gcgccggagc gaagaagggg gcctggcggc ccccttcttc gacgatcccg gtggttagac
     1261 cgtgaacgaa ccgcccgcgc ccgcgatgaa acgcgcggcc gtcgggatga agatgtcaca
     1321 ggtcgtggcg ccgtgatggt tctgcggtcc ggtcggcagg gcgggacttt ggttcttgtt
     1381 tcctgtctcg ccggacgcgt cgtagcggtt caacccgtag ttcgggttga cctgatagtc
     1441 attgtccgcg ccgccgggtc ccttgccgat cgtgacatag gccggagtca cgggcgactc
     1501 gttgctggtg aagtactcgg ctgccgtcgc gaagttcgga cagttcgggc cgggcagacg
     1561 ctcggtctgc ggcgtgaacg ttccgtagtc gtaggccgcg tacggagccc agtcgaccag
     1621 caggttctgg cccgctcccg tcggcccggg gccaccggag ctttgcaggt ccggcgtgcc
     1681 gatgaagacc ccgccgtgcg tggctgcctg gacaccgacg atctggtcgc cgggcgccgc
     1741 gatctcgtag cagttgacga tgttggacag gttgatccag cagtagttga acggcaccga
     1801 catcgtcatc gtgttgcccg agtagctcag gttcacgccg gtccccggga agttgacggt
     1861 ctgatgcgtc gccggcgttc ccgagcagaa gcggctcagg acgttcgact gcggcgcgtc
     1921 ggccgaggtg cgggtctggt acaggatgtt gccgaccggg tcccagcggc cccacccgtg
     1981 gttacggacc tggtaggtac ccgtcgggcc gaggtggacg tcccagtaca agaagaagta
     2041 ctcgttgttg ccggtcaggt acgggccggc gtgcgagaag cgcgtgcagt ccggcgcgtc
     2101 cccgataatc tgccgcgcat tctgacggcg ggggttctgg aagaacacgt agtagccgaa
     2161 ctcaccgcca ccggcagccg caagcgtcga cggcggggtg cccggtcgcg gcagctgacg
     2221 cgtcgtcatc ttccaggtca gctttccggg cacagtcgtc gagtcgagcg agaacgactg
     2281 gatgtccgcc tcgcccaggc agccgcgcgg gttcggcgca ccgttttgca ccggcggacc
     2341 gttgttgtag gcgaagaagt tcttgttggg ggacagaacg gcgttcagcg tgtcgcagtt
     2401 cgaaaccgtt ccggccgcgt tgacgctctt gccgccgagg gcggtgtccc aggcgggcgg
     2461 gttcgcggtg ggattgtcgt tgatggtcac gccaccggcg ctcgcgctgt tcggcacgaa
     2521 gatcgacgcc gccagcgccg ccatcgcgac agcgacggcg aaagtccttt tcactcgctt
     2581 cacggtgagc ctcctcatcc tgacgggatc gatctagacg acggtgacga cgaccgtggc
     2641 cgtcgcgccg acgttgtctg cggcatcgac cggggtggcg tcgacgagat actcgccggg
     2701 caccaaggcg aatgccggaa cgtactccca cgacccgtcg tccgcgatgc tcgcgtggcc
     2761 ggactcgctg aggttgagcg tcgtgtgcgt gaggttgacg gcgatggtga cgacacccga
     2821 gccgtcgtcg gacgacgttc cttcgacccc gtcgccgccg atgaactcgt tgaccgtggg
     2881 aaggttcagc aacgtagagc cctgcggcgt ggtgatgctc agcgacggcg ccaccgcatc
     2941 cacggtgacc gtgaccggag cggagttcgt gacgttgtcg agcgagtcgg tcatgcgggc
     3001 gacgagctga atggtggtgt catccgagcc ggtcagcgcg aactcgaacg gctcgctgat
     3061 gacactgccg gccgagatgc cgtcgacgag tagctcgacg gatgcgactc cgtcgtcgtc
     3121 gtcggcagca accaggacgg tgaacgagcc gggaacgtat cccccggtcg gcgaaaggat
     3181 atcgacgctc gagcttgcag aagccggcgc catacccagc attccgacca agacgcccgc
     3241 gagaacgacg agcgcactat ggcggcgcat gtgatccccc cttcgatggc aagacggaat
     3301 tgtctccctc ggcggcccta ttcgccacca acccgaggga ttcctttcga aagggaacat
     3361 tgcgacgcga gcaagcaacc cggccggcgg ctcggcgtgc cggcgctcag tggatgcggt
     3421 aggccttggg gaagctcttg gccaggacgg cgccgccggt ggcgcgaagc tccacgaaga
     3481 acgtcaccgt cttggtcccc ggcgagggca cgtaggacag ccaggcgccg ccatgggggc
     3541 acccgctctc gaagcacagc cggttcgggc ccttctcggc cggcagcagc cggattgcgg
     3601 ccggggtggt tgacttgttc tcgtaggcga ccagcgcgat ctgggccttg tcctgggtcg
     3661 acaggtacgc gaccccctcg aacgggaccc ggcaggcgct tccgacacgc gggacgaagc
     3721 aagcacttcc ccacgcctcg tcctcgggaa cctccgcctt cacgaagtcc ggaac
//
LOCUS       DATLFO010000187         6310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_57066, whole genome shotgun sequence.
ACCESSION   DATLFO010000187 DATLFO010000000
VERSION     DATLFO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57066"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..626
                     /gene="atpF"
                     /locus_tag="VM841_07475"
     CDS             93..626
                     /gene="atpF"
                     /locus_tag="VM841_07475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVL90057.1"
                     /translation="MVLNILAAEETQFILPHADELIWGSIAFVLLFVALSVFAFPKLK
                     ETMAARTERIQGQLQSAEKAKTEADAVLEQYRAQLADARNEANRIIEEAKKTAESLRR
                     ELVAKAEQEAAEIVTRARAEVGSERDRAMSELRTSVASLTIDVAEKVIGRELQNEAAQ
                     KAFVDQTITELSRMGQN"
     gene            626..1159
                     /gene="atpH"
                     /locus_tag="VM841_07480"
     CDS             626..1159
                     /gene="atpH"
                     /locus_tag="VM841_07480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HVL90058.1"
                     /translation="MDPKLEGYAAALFEVARAEGVLDDVEDELFRFARTVEAESKLRE
                     ALQDQALPPELRMKVIGELLGSRANPHTVNLLQFIVGAGKARQLSQIVDALVARAASE
                     RSKSVAEVRSAIPLGDEQRSKLRDAIKAATGKDVDMKVIVDPKVLGGLHVRVGDQVFD
                     ATIRRRLELAQESLGKS"
     gene            1174..2805
                     /gene="atpA"
                     /locus_tag="VM841_07485"
     CDS             1174..2805
                     /gene="atpA"
                     /locus_tag="VM841_07485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVL90059.1"
                     /translation="MSELTIRPEDIAAVLKAQVESFRPATTMEQVGRVTETGDGIARI
                     SGLPGAMANEILEFEGGLLGLALNLEADQIGAVVLGDATQIEEGDPVKATGRILSVEV
                     GDAMLGRVVNAIGQPIDGKGPLDRSKIAGRRALELQAPTVVQRSPVNEPLQTGVKAVD
                     AMTPVGRGQRQLIIGDRQTGKTAVAIDAIIAQRAVWGTKKQVKCVYVAIGQKASTVAE
                     VVAALEANGAMEYTCVVVAGASDPAPFKFLAPYTGSAISQQWMYNGEHALVVYDDLSK
                     QAEAYRELSLLLRRPAGREAYPGDVFYLHSRLLERAAKLSPELGSGSATALPIVETKG
                     GDVSGYIPTNVISITDGQIYLVTDLFFAGVRPAINVGISVSRVGGNAQIKAMKKVAGR
                     LRLDLAQFRELEAFAAFGSELDKASQQQLDRGARVVEILKQPQFTPMTPEDQVISIYA
                     VTNGFMDDIPVPDCSRFEKELLEYMHSRKPQVPQTISETGAMDDATEKALREGIEDFK
                     RGFATTEIAPIVTEEPTGPNAGWDRMEAQFEEVTE"
     gene            2807..3709
                     /locus_tag="VM841_07490"
     CDS             2807..3709
                     /locus_tag="VM841_07490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HVL90060.1"
                     /translation="MAGQLRVLRRRIRSVQSTMKITRAMELIAASRIVKAQRRVDEAK
                     PYALRITEAIRELAGVSEVMATFPLLQEREIKRVGVVVITSDRGLAGGYNSAVIKVAE
                     QTLKKIAAEGKEYRLHLAGKKGISYFKFRNYDIATQVTGVSDTPTYDDAARIGRRLMS
                     EFESGEIDQVLVCYTDFRSATTQVAGKTQILPIQREEIAAKETGVPALYEFEPEPGEI
                     LSLLLPRYVITVVYAAMLESSAAEHAARRRAMKVATDNADELIGILQREANRQRQAEI
                     TTEILDIIGGAEALRKQKETAAAH"
     gene            3745..5169
                     /gene="atpD"
                     /locus_tag="VM841_07495"
     CDS             3745..5169
                     /gene="atpD"
                     /locus_tag="VM841_07495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HVL90061.1"
                     /translation="MTEPQKTALADGRVIQVIGPVVDVEFPPANLPEINFALQIERDV
                     EGVKDLITAEVAQHIGESTVRAICMLPTDGLRRGAKVHNTGEAITVPVGDVTLGHIWN
                     VLGRSLDQPDIKVTERWPIHRDAPPFDELEPKTEMFETGIKVIDLLEPYVKGGKIGMF
                     GGAGVGKTVVIMEMINRVATQHGGVSVFAGVGERTREGTDLMLEMKETGVIEKAALVY
                     GQMDEPPGVRLRVALAALTMAEYFRDVQRQDVLLFVDNIFRFTQAGSEVSTLLGRMPS
                     AVGYQPTLAGEMGELQERITSTKGRSITSLQAIYVPADDITDPAPHTTFAHLDATTVL
                     SRQIAELGIYPAVDPLDSTSRILDPRYVGQEHYDVARAVQTTLQRYKDLQDIIAILGI
                     DELAEEDKLIVGRARKIQKYLSQPFFVAEQFTGIPGTYVKTQDTIEAFKAITKGEMDS
                     VPEQAFYMCGGIDDVLAKQREMEK"
     gene            5172..5582
                     /gene="atpC"
                     /locus_tag="VM841_07500"
     CDS             5172..5582
                     /gene="atpC"
                     /locus_tag="VM841_07500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HVL90062.1"
                     /translation="MAATEATMTVQVVTPDRQVVAAEDATFVLAHGIAGDVGIMAGHA
                     PLLIALGVGPLRVDSGERKTWMVVDGGFLQVSRNQVIILAEYAIMPNEVETSWVASEI
                     REMEAALAKGENEEYRGRLNRAKAIEAVHGIAKM"
     gene            6188..>6310
                     /locus_tag="VM841_07505"
     CDS             6188..>6310
                     /locus_tag="VM841_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90063.1"
                     /translation="MDHEKLLVHGGVALNGRVAAGGAKNSALKLMAATLLAEGPY"
BASE COUNT         1151 a         2097 c         2117 g          945 t
ORIGIN      
        1 cctcgggttt gcgctcgtcg aggcgctcgc gctgttcggc ttcgtgctct tcttcatcgt
       61 cccgagcaac taacggccgt taggagcagg aaatggtttt gaacattctg gcggcggaag
      121 agacgcagtt catccttccg cacgcggacg agctgatctg gggctcgatc gcgttcgtgc
      181 tgctgttcgt cgcgctttcg gtcttcgcgt tcccgaagct gaaggagacg atggccgcgc
      241 gcaccgagcg gatccaggga cagctccaat cggcggagaa ggccaagacc gaggccgacg
      301 ccgtgctcga gcagtaccgt gctcagctcg ccgacgctcg caacgaggcc aaccgcatca
      361 tcgaagaggc gaagaagacg gccgagtcgc tgcgccgcga gctggtcgcc aaggccgagc
      421 aagaggccgc ggagatcgtc acacgcgccc gcgccgaggt cggctcggag cgagaccgcg
      481 cgatgagcga gcttcgcaca tcggtcgcct cgctcacgat cgacgtcgcc gagaaggtca
      541 tcggccgcga gcttcagaac gaggccgcgc agaaggcctt cgtggaccag acgatcaccg
      601 agctgtcgcg gatggggcag aactgatgga tccgaagctc gaagggtacg cggccgcgct
      661 gttcgaggtc gcccgggcgg aaggggtgct cgacgacgtc gaggacgagc tcttccggtt
      721 cgcgcgcacc gtcgaggccg agtcgaagct gcgcgaggcg ctgcaggacc aggcgcttcc
      781 gccggagctt cgcatgaagg tcatcggcga gctgctcggc agccgcgcga acccgcacac
      841 ggtcaacctg ctccagttca tcgtcggcgc cggcaaggcc cggcagctgt cgcagatcgt
      901 cgatgcgctc gtcgcccgcg cggcgtcgga gcgctcgaag tccgtcgcgg aagtgcgctc
      961 cgccatcccc ctcggcgatg agcagcgctc gaagctgcgg gacgcgatca aggcggcgac
     1021 cggcaaggac gtggacatga aggtgatcgt ggatccgaag gtgctcggcg gccttcacgt
     1081 ccgggtcggc gaccaggtct tcgacgccac gatccgccgg cgtctggaac tcgcacaaga
     1141 atctctaggc aagagctaag gaagaggaag ctgatgtccg agctgacgat ccgtccggaa
     1201 gatatcgccg cggtactcaa ggcacaggtc gagtcgttcc gtccggccac gacgatggag
     1261 caggtcggcc gcgtcaccga aaccggcgac ggcattgcgc gcatctccgg gctgccgggc
     1321 gccatggcca acgagattct cgagttcgag ggcgggctgc tcggtctggc cctcaacctc
     1381 gaagcggacc agatcggcgc cgtcgtgctc ggggacgcca cccagatcga agaaggcgac
     1441 ccggtcaagg cgacgggccg gattctttcg gtcgaggtcg gcgacgccat gctcggccgc
     1501 gtcgtgaacg cgatcggcca gccgatcgac ggcaagggcc cgctggaccg gtccaagatc
     1561 gccggccgcc gcgcgctcga actgcaggcg cccaccgttg tgcagcgctc gccggtcaac
     1621 gagccgcttc agaccggcgt gaaggccgtg gacgcgatga cgcccgtcgg ccgcggacag
     1681 cgtcagctca tcatcggcga ccgtcagacc ggcaagaccg ccgtcgcaat cgacgcgatc
     1741 atcgcgcaac gcgccgtgtg gggcaccaag aagcaggtca agtgcgtcta cgtcgccatc
     1801 ggccagaagg cctcgacggt ggccgaggtc gtggccgcgc tcgaggcgaa cggcgccatg
     1861 gagtacacct gcgtcgtggt cgccggcgcg tcggatcccg caccgttcaa gttcctcgcg
     1921 ccctacaccg ggtcggcgat cagccagcag tggatgtaca acggcgagca cgcgctggtc
     1981 gtctacgatg atctgtccaa gcaggccgag gcctaccgcg agctgtcgct gctgcttcgc
     2041 cgtcccgccg gccgcgaggc ctatccgggc gacgtcttct accttcactc gcgtctgctc
     2101 gagcgcgcgg cgaagctctc gcccgagctc ggatcgggat cggcgacggc gctgccgatc
     2161 gtcgagacca agggtggcga cgtgtcgggc tacatcccca cgaacgtcat ctcgatcacc
     2221 gatggtcaga tctatctggt gaccgacctg ttcttcgccg gtgtgcgtcc cgccatcaac
     2281 gtcggcatct cggtgtcgcg cgtcggcggc aacgcgcaga tcaaggccat gaagaaggtc
     2341 gccggccgcc tgcgtcttga cctcgcgcag ttccgcgagc tcgaggcgtt cgccgcgttc
     2401 ggctccgagc tggacaaggc gtcgcagcag cagctcgacc gtggtgcgcg cgtcgtcgaa
     2461 atcctcaagc agccacagtt cacgcccatg acgcccgaag accaggtcat ctcgatctac
     2521 gcggtgacca acggtttcat ggacgacatc ccggtgccgg actgctcgcg cttcgagaag
     2581 gagctcctcg agtacatgca ctcgcgcaag ccgcaggtcc cgcagacgat ctccgagacc
     2641 ggtgccatgg acgacgcgac cgagaaggcg ctgcgcgagg gcatcgagga cttcaagcgc
     2701 ggcttcgcaa ccaccgagat cgctccgatc gtcaccgaag agccgaccgg ccccaacgcg
     2761 ggctgggacc gcatggaggc gcagttcgag gaagtgaccg agtaacgtgg ccgggcagct
     2821 tcgcgttctt cgccggcgga ttcgctccgt ccaatcgacg atgaagatca cgcgtgcgat
     2881 ggagctcatc gccgcgtccc gaatcgtcaa ggcgcaacgg cgtgtcgatg aagccaagcc
     2941 gtacgcgctt cgcatcaccg aggcgattcg cgagctggcc ggcgtcagcg aggtcatggc
     3001 cacgttcccg ctgctgcagg agcgcgagat caagcgggtg ggcgtcgtcg tgatcacttc
     3061 ggaccgcggt ttggcgggcg gctacaactc ggccgtgatc aaggtggccg agcagacgct
     3121 caagaagatc gcggccgagg gcaaggagta ccgcctgcac ctggccggca agaagggcat
     3181 ctcgtacttc aagttccgca actacgacat cgccacccag gtgaccgggg tgtccgacac
     3241 cccgacctat gacgacgcgg cacggatcgg gcgccggctg atgtcggagt tcgagtcggg
     3301 cgagatcgac caggtcctcg tctgctacac cgacttccgc tccgccacca cgcaggtggc
     3361 cggcaagacg cagatcctgc cgatccagcg ggaagagatc gcggcgaagg aaaccggcgt
     3421 tccggcgctc tacgagttcg agccggagcc gggcgagatc ctgtcgctgc tgctgccgcg
     3481 ctacgtcatc acggtcgtct acgctgcgat gctcgagtcc agcgcggccg agcacgccgc
     3541 gcgtcgccgc gcgatgaagg tcgcgaccga caacgccgac gagctgatcg gcatcctgca
     3601 gcgcgaggcg aaccgccagc ggcaggccga gatcaccacc gagatcctgg acatcatcgg
     3661 cggcgcggaa gcgctgcgga agcagaaaga gaccgcggcg gcgcactagc gcccggcgat
     3721 acaaggagac ccacagacat gacgatgacc gaaccgcaga agacggcgct ggcggacggc
     3781 cgcgtgatcc aggtgatcgg gccggtcgtc gacgtggagt tcccgcccgc gaacctgccc
     3841 gagatcaact tcgcccttca gatcgagcgc gacgtcgaag gcgtcaagga cctgatcact
     3901 gccgaggtcg cacagcacat cggcgagagc accgtccgcg ccatctgcat gcttcccacc
     3961 gacgggcttc gccgcggggc caaggtgcac aacaccggcg aggccatcac ggtgccggtc
     4021 ggcgacgtca cgctcggcca catctggaat gtgctcggcc gctcgctcga ccagcccgac
     4081 atcaaggtca ccgagcgctg gccgatccac cgcgacgcgc cgcctttcga cgagctcgag
     4141 cccaagaccg agatgttcga gaccggcatc aaggtcatcg atctgctcga gccctacgtc
     4201 aagggtggaa agatcgggat gttcggcggt gcaggcgtcg gcaagaccgt cgtcatcatg
     4261 gagatgatca accgcgtcgc cacgcagcac ggtggtgtgt cggtgttcgc gggcgtcggc
     4321 gagcgcacgc gtgaaggcac cgacttgatg ctcgagatga aagaaaccgg ggtcatcgag
     4381 aaggcggcgc tggtgtatgg ccagatggac gagccgccgg gcgtgcgtct gcgcgtcgcg
     4441 ctggccgcgc tgaccatggc cgagtacttc cgcgacgtgc agcgtcagga cgtgctgctg
     4501 ttcgtggaca acattttccg cttcacgcag gccggctccg aggtgtccac gctgctgggc
     4561 cgcatgccgt cggccgtcgg ttaccagccg accctggccg gcgagatggg cgagctgcag
     4621 gagcgcatca cttcgacgaa gggccgctcg atcacctcgc tgcaggccat ctacgtgccc
     4681 gccgacgaca tcaccgaccc ggcgccgcac acgaccttcg cgcatctgga cgccacgacc
     4741 gtcctttcgc gtcagatcgc cgagctgggc atctacccgg ccgtggaccc gctcgactcg
     4801 acctcgcgca tcctggatcc gcgctacgtc ggtcaggagc actacgacgt cgcgcgtgcg
     4861 gtgcagacga cgctgcagcg ctacaaggac cttcaggaca tcatcgcgat tctgggtatc
     4921 gacgagctcg ccgaagagga caagctgatc gtcggccgcg cccgcaagat ccagaagtac
     4981 ctgtcgcagc cgttcttcgt cgccgagcag ttcaccggca tccccggcac ctacgtcaag
     5041 acccaagaca ccatcgaagc gttcaaggcc atcaccaagg gcgagatgga cagcgtgccg
     5101 gagcaggcct tctacatgtg cggcggcatc gacgacgtgc tggccaagca gcgcgagatg
     5161 gagaagtaag cgtggccgca accgaagcga cgatgaccgt ccaggtcgtc acgcccgacc
     5221 gtcaggtggt ggccgccgag gacgcgacgt tcgtgctggc gcacggcatc gccggggacg
     5281 tgggcatcat ggcgggccac gcgccgctgt tgatcgcgct cggcgtcggg ccgcttcgcg
     5341 tggattcggg cgagcgcaag acctggatgg tcgtggacgg cggcttcctt caggtcagcc
     5401 ggaaccaggt catcatcctg gccgagtacg cgatcatgcc caacgaggtc gaaacgtcgt
     5461 gggtcgcgag cgagatccgc gagatggaag ccgcgctggc caaaggcgag aacgaggagt
     5521 atcgcggccg cttgaaccgc gccaaggcaa tcgaagccgt gcacggcatc gccaagatgt
     5581 aagcgctcgc tacgcgcgca ccggcagatc gagccacaac gcgaacgcac catactgcgc
     5641 ctcgccggca gcctgccctt tcccgtgagc ggcagggtgg tccacgaatg agccgtcgac
     5701 caggtgccgc cgaccaccac tcccggccgg atcaggccgc ctgtgaacgc tcccatccgg
     5761 ggcgtctcgc ccggcgcgtc ggagcgccag atgtggtccg agaagaccgc tctgccccgg
     5821 atggtcgccg tgacttgacg ggaaggcgcg ggcaggccga cgaaagagaa cccggccgcc
     5881 gatgtgaccg acgatggtcc cgagatggtg accacgtagt cgtctccggc tttcatatag
     5941 ggcttccatg acgtggacgt gaagatcgat tcgcagtagc cccgattcgt ttgtcccggc
     6001 gggcatccca ccgcgccgcc cagcccgagg ccggtccacc cggccgcagg ggcagcagca
     6061 gcatcgcgag catggcgaca acgagtgttc tgagtctcat cactccataa cgggcccgcc
     6121 gtcgcccaca ccgacggcag cgggccccat ccctttggga agcaaccgac ttgggtaaga
     6181 tcgacccatg gatcacgaga agctcctcgt tcacggtggc gtcgccctga acgggcgtgt
     6241 cgcggccggg ggagcgaaga attcggcgct gaagctgatg gccgcgacct tgctggccga
     6301 aggcccgtat
//
LOCUS       DATLFO010000188         3071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_142073, whole genome shotgun sequence.
ACCESSION   DATLFO010000188 DATLFO010000000
VERSION     DATLFO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142073"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VM841_07510"
     CDS             complement(<1..2045)
                     /locus_tag="VM841_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90064.1"
                     /translation="MRDRAHLRTAVAALLALLGSAVPGRALREAPRASDLFVPGEVMI
                     QFSSPAAAAKAEGALRRAGATLAGRIDQLHTLLVRVPGDATAAATVMRSIPGVEAAEV
                     NLIGRVQGAPPPSRANDPLVKGIGPTFGDRQWYLNRINAPYSWMVHPSTYYSARTKVH
                     AFRDLEAVKIAVVDTWIDTDHNDWKNAGGVSTDADNGGQLALDDAIMLQPAKDLRGPI
                     DWHGTHVAGIIGASTDNELDIAGIAYSAQIMPVVVARGDGMTNAFAIAKGISMAVDRG
                     AKVVNVSLAIQNQKANQVSVLRSAVNRAVAKGAVVVAAAGNDRSGDDAEYPAGFAAEN
                     AGVIAVGASDFYDERAFCSKKGRHISVVAPGVLMWGLDPQNHRDSRDHTIRREVCGTS
                     YAAAVVSGIAGLVAGKYRGIKPVDIARRIAQGADDLGKPGRDDDTGAGRVNAERALWP
                     GGGAGSSWLAVPVAGEKGHVDVLAAAQSFRGVTQAEITVDGMQRGKGIAMRPTDGRWG
                     SRGERLIARLDTSKLAEGPHELHVRAKDASGRWGPASSSVMIVDRTPPTLAGVTCYPQ
                     AAVYPDDPSAAIRFSIADAWSPHMSVKIVLLDPSFTPAVEMVYPSVPTGTYGRPLWSE
                     IEAAAGGPITPGLYRLIVTVKDMAAHEVWSESPCLVLPTTRAVPSPNASNGAGGLPG"
     gene            2231..>3071
                     /locus_tag="VM841_07515"
     CDS             2231..>3071
                     /locus_tag="VM841_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HVL90065.1"
                     /translation="MVDVLFVCYGNICRSPMAEALLDARLNQLLGPEHDVVVGSAGVG
                     AADGGRATRESREAMAARGLDLSGHRSQRLTPALARRARLVFCMDEEQAAVIRSMAPD
                     ADVHVLAGGVEDPIGCGAWVYEAVAHKIEAAVEQIATGIAEARGVVPAAVRNATDAPQ
                     PAASPALEGESLMRVVLGSDHAGFRYKELYRDHLIKAGHDVIDLGVDTDEVPADYTQI
                     SAAVAHTVARGDAERGIIVAGSGNGEAIVANKIAGIRAAVCNDMYTAEMARRHNDANV
                     LCVG"
BASE COUNT          464 a         1085 c         1053 g          469 t
ORIGIN      
        1 atgcccggca ggccgcccgc accgttggac gcgttcggcg aaggcaccgc gcgtgtcgtc
       61 ggcagcacca ggcacgggga ttccgaccag acctcgtgcg cggccatgtc cttcaccgtc
      121 acgatcagcc ggtacaagcc cggcgtgatc ggcccgcctg ccgccgcctc gatctcggac
      181 cacagcggac ggccgtacgt gccggtcggc accgatggat agaccatctc gaccgcgggc
      241 gtgaacgacg ggtcgagcag aacgatcttg acgctcatgt gcggcgacca cgcgtcggcg
      301 atcgagaagc ggatcgcggc cgacggatcg tcgggataaa cggctgcctg cgggtagcag
      361 gtgacgcccg caagcgtcgg gggtgttcga tccacgatca tcacgctcga tgaggccggg
      421 ccccaccggc cgctcgcgtc cttggcccgc acgtgaagct cgtgtgggcc ttcggcgagt
      481 ttcgaggtat ccagacgcgc gatcagccgc tcgccccgcg agccccaccg cccgtccgtc
      541 ggccgcatcg cgatgccctt gccacgctgc atgccatcca cggtgatctc cgcctgcgtc
      601 acgccgcgga agctctgcgc ggcggcgagc acgtccacgt ggcccttctc gcccgcgacc
      661 gggaccgcca gccacgacga gcccgcgccg ccgcccggcc acagcgcccg ctcggcgttg
      721 acgcgacccg cgccggtgtc gtcgtcgcgg ccgggcttgc cgagatcgtc cgcgccttgc
      781 gcgatgcgtc gtgcgatgtc caccggtttt atcccgcggt acttgcccgc gaccaggccg
      841 gcgatgccag acacgaccgc ggcggcgtag gaggttccgc agacctcgcg gcgtatggtg
      901 tgatcacggc tgtcgcgatg gttctgagga tccaggcccc acatcagcac gccgggagcc
      961 acgaccgaga tgtgacgtcc cttcttcgag cagaacgcgc gctcgtcgta gaaatcagag
     1021 gcgccgaccg cgatcacacc ggcgttctcg gccgcgaaac cggccggata ctccgcgtcg
     1081 tcgccgctgc ggtcattgcc cgcggccgcc acgacgaccg cgcccttcgc gaccgcccgg
     1141 ttcacggccg agcgaagcac cgacacctga ttggcctttt gattctggat cgcgagcgag
     1201 acgttgacca ccttcgcgcc acggtccacc gccatcgaga tgcccttggc gattgcgaat
     1261 gcgttggtca tcccgtcgcc gcgcgcgacc acgaccggca tgatctgcgc cgagtacgcg
     1321 atcccggcga tatccagctc gttgtcggtc gaggcgccga tgattccggc gacgtgcgtg
     1381 ccgtgccagt cgatggggcc gcgaaggtcc ttcgccggct ggagcatgat cgcgtcgtcg
     1441 agcgcgagct ggccgccgtt atcggcgtcg gtcgagaccc cgcccgcgtt cttccagtcg
     1501 ttgtgatcgg tgtcgatcca cgtgtccacg accgcgatct tgaccgcctc gaggtcacgg
     1561 aacgcatgca ccttcgtgcg cgccgagtag tacgtgctcg ggtgcaccat ccacgaatac
     1621 ggcgcgttga tcctattgag gtaccattgc cggtccccga acgtgggacc gattcccttc
     1681 accagcggat cgttggctcg ggagggcggc ggtgcgcctt gcacgcggcc gatcaggttc
     1741 acctcggcgg cctcgacgcc aggaatggac cgcatcacgg tcgcggccgc ggtcgcgtcg
     1801 ccgggcacgc gaaccagaag cgtgtgcagc tggtcgatcc ggccggcgag cgtggcgccc
     1861 gcacggcgca gcgcgccttc cgccttcgct gccgcggcgg gtgaggagaa ttggatcatc
     1921 acctcgccag gaacgaacag atcggaggcg cgcggggcct cgcgcagcgc gcgtccgggc
     1981 acggcgctgc ccagcagcgc caacagcgcc gcgacggccg tgcgaaggtg cgcgcgatct
     2041 ctcatatgcg tctctcgtgg ggacactacg aagcggaggc ctccgggccc tgcccgcatg
     2101 ctcggatcgg agggttttgc ccgcctcgca taccgaaccg gacggaagaa cgcgccgaag
     2161 cgaccgcccg ttttcggcgt ggcgatgcgg cccggaggcg cgcggtcgct ctgttagact
     2221 cgccgcgttg atggtcgacg tcctcttcgt ttgctacggc aatatctgcc gttcgccgat
     2281 ggccgaagcg ctgctcgacg cgcgcctgaa tcagctgctc ggccccgagc acgacgtcgt
     2341 cgtggggtcc gccggagtgg gagcggccga cggaggacga gcgacccgcg aatcgcgcga
     2401 ggccatggcc gcacgcggcc tcgacctgtc cggccatcga tcgcagcggc tcacgcccgc
     2461 gctcgcgcgc cgggcgcgcc tcgtgttttg catggacgag gagcaggccg cggtcatccg
     2521 ctccatggcc ccggacgcgg acgtgcacgt gctcgccggc ggcgtcgagg atccgatcgg
     2581 ttgcggcgcc tgggtctacg aggcggtcgc gcacaagatc gaagccgcgg tcgagcagat
     2641 cgcaaccggg atcgccgagg cccgcggcgt tgtccctgcc gccgtgcgga acgcaacgga
     2701 tgcaccgcag ccggcggcgt cgccggcatt ggagggggag agcctgatgc gcgtcgtgct
     2761 gggaagcgac catgccggat tccgctataa ggagctgtac cgcgaccatc tgatcaaggc
     2821 cggccacgac gtgatcgacc tgggcgtcga taccgacgag gtgccggccg actacaccca
     2881 gatctccgcc gcggtcgcgc ataccgtcgc ccgcggcgat gcggagcgcg ggatcatcgt
     2941 cgccggatcc ggcaacggcg aggccatcgt ggcgaacaag attgccggga tccgcgcggc
     3001 cgtgtgcaac gacatgtaca ccgccgaaat ggcgcggcgt cacaacgacg caaacgtgct
     3061 ctgcgtggga c
//
LOCUS       DATLFO010000189         4875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_57083, whole genome shotgun sequence.
ACCESSION   DATLFO010000189 DATLFO010000000
VERSION     DATLFO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57083"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2143)
                     /locus_tag="VM841_07520"
     CDS             complement(<1..2143)
                     /locus_tag="VM841_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL90066.1"
                     /translation="MPDGRVSPGVVRSLAASRSFSSLPPEHLRAIASQARLVEVARGR
                     TLIKQGSDGGDVYVVLGGRLRVEVDGDREVLSHLSPGDVIGEIAFMTGAKRAATVRAE
                     IKSRVARFTPTRFAQLLHDYPELEERLAEEAHRRLLDKHLATDLAALFGPLDERTRAA
                     IGSMVEWVALPAGEKLYDAGAPSDDAFVVSFGRVRLTTPDGTSEERGHGQLAGAEALI
                     DHAPRAESAYAIRDSYLVRLPKLAFDVISRRHPGAVTGIARAIVRRHSGAPVRQGDDR
                     VTIAVVPASDSVDVRLVASRVAECLRVYGPTMHVWSARADDALNRAGIAQSGDDAAGL
                     RLTQWLLEAEDANRFVVYEPDRSPTSWTRRTVRQADVVLLVANGRDHAPSMLEGALAD
                     LLRGEQRPRTVLAILQPDGVDLPSGTSRWLEHRDVDDVTHIREGSPSDFERLARLASG
                     RPVALVLGGGGARGFAHLGVIRALRDLGIPIDAIGGASIGSIMAIGPASGVEADEMID
                     ITAKRFAKLIDYTLPFVSIIKGRRITRAMHEQLGDHHVEDLWIPYYCITTNLTRATEV
                     THTRGPIVPLIRSSAAIPGFLPPVRLGEDLHIDGGVLNNVPTDVMRRRFPGATIITVD
                     VSPPVGPRAKAEFGLWVSGSQSLRAKIRRRHGPPGLTETLIASTVVASMRKRDRNVAS
                     GLADLWMHLDTRGIGLLDFERVRPTAQKGYDE"
     gene            complement(2211..3146)
                     /locus_tag="VM841_07525"
     CDS             complement(2211..3146)
                     /locus_tag="VM841_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90067.1"
                     /translation="MKPVALVVCLLAALLPGSWARASATIDIDVRSNFFAPDVARPVQ
                     GDTVRWTVRTGAHDVVTYAGAMSFASDMMSPSSPPYSRAYTGGEVLYRCTPHSTVSSS
                     GICTGMCGVLTDRTAPPAAPVVAEPAPGATVPAGSVTFSGTAEPWVIVRLTGGGATLG
                     EVLAAQNGAWSIVRSMAGGTHTVTALALGADGSQGGSVTRTFTVGGAPDGTPPEVTIH
                     SGLVSGGAGRVFLFGDASDDVAVARIRIRSSDAIGRLLSEVVADCPECAAASVWWRVS
                     VSLPPGVATVHAVADDLAGNASAPAGPVYAVVFPG"
     gene            complement(3143..4654)
                     /locus_tag="VM841_07530"
     CDS             complement(3143..4654)
                     /locus_tag="VM841_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90068.1"
                     /translation="MRRILVAPALLAALLAACLPARSQVAAPYRVGAAVRDISPIGWG
                     TINTGGYGFGDGSSPVSQALGRGGRSSFQGNHIAARALVVEDAATGVAVAMVSVETQG
                     MFAAYQNDPESGLDAIAQRIHAAVPALPPANVTISNDHTHAGPDAIGVWGFIPSDYLE
                     HIASRTVDAVVAAYQARRGAVIVAGASDAPDLIYNQTCTEALNQSPDSNFPNTVCDPF
                     LESKDSWVRVLQARDAGNGSVIATAMSYAAHATLGGGAGVHGDWPQFMSEALTQRYGG
                     TGIAYQGTNGRTQPCRPRCSFTDKRKPGYEIADRVKSYTTMLMYHVEQAMTGAPEVQG
                     PVAGAQTMIRHNVENPIVGSLLFKGDAVGAPIQRSQQSPWLTGTTVGTIVSALRIGDL
                     LISGAPGEPYPNIAAGVAEATNVPPQRHWTLALADDQLGYLIAPVEAWPAVASQVAIN
                     DNVIFNVSPTIGDHVMCAQIRMARAVGFTFSRIAPDARCAAWDAYDAAEAAVS"
BASE COUNT          723 a         1713 c         1745 g          694 t
ORIGIN      
        1 cctcatcgta gcccttctgg gcggtcgggc ggacccgttc gaagtcgagc aggccgatgc
       61 cgcgcgtgtc caggtgcatc cacaggtcgg ccaggccgct cgcgacgttc cggtcgcgct
      121 ttcgcatcga cgcgacgacg gtggatgcga tcaaggtctc ggtcaggccg ggtgggccgt
      181 gccgccggcg gattttggcg cgcaggctct gcgatcccga gacccacagg ccgaactctg
      241 ccttggcccg ggggccgacc ggcggggaca cgtccacggt gatgatggtg gcgccgggga
      301 agcggcggcg catcacgtcg gtcggcacgt tgttgagcac gccgccgtcg atgtgcaggt
      361 cctcgccgag ccggaccggc ggcagaaagc ccgggatcgc ggccgacgag cggatcagcg
      421 gcacgatcgg tccgcgcgtg tgcgtcacct cggtggcgcg cgtcaagttc gtggtgatgc
      481 agtagtacgg aatccaaagg tcctcgacgt ggtggtcgcc gagctgctcg tgcatcgcac
      541 gggtgatcct gcgtcccttg atgatcgaca cgaacggcag tgtgtagtcg atcagcttcg
      601 cgaagcgctt ggcggtgatg tcgatcatct cgtcggcttc cacacccgac gcgggaccga
      661 tcgccatgat cgacccgatg ctcgccccgc cgatcgcgtc gatggggatg cccaggtcgc
      721 gaagcgcgcg gatgacaccc aggtgcgcga accctcgcgc cccgccgccg ccgaggacca
      781 gcgcgaccgg ccgccccgag gcgagccgcg cgagacgctc gaagtcggaa ggcgatcctt
      841 cgcgaatgtg cgtgacatcg tccacgtcgc ggtgctcgag ccagcggctc gtcccgctgg
      901 gaaggtccac gccgtcgggc tgaagaatcg cgagcacggt ccgcggccgc tgctcgccgc
      961 gcagcagatc ggccagcgct ccctcgagca tgctcggagc gtgatcgcgg ccgtttgcga
     1021 ccagcaagac cacgtcggcc tgccggacgg tgcggcgcgt ccacgaggtc ggcgaccggt
     1081 ccggctcgta gacgacgaag cggttcgcgt cctcggcctc gagcagccat tgggtcaggc
     1141 ggagtccggc cgcgtcgtcg ccgctctgag cgatgccggc acgattgagc gcatcgtcgg
     1201 cacgcgcgct ccacacgtgc atcgtggggc cgtagacgcg aaggcattcc gcgacgcgcg
     1261 aggcgacgag ccgcacatcc accgagtcgc tcgccggcac caccgcgatc gtcacgcggt
     1321 cgtcgccctg gcggaccggc gcgccgctgt gacggcgcac gatggcacgc gcgatcccgg
     1381 tgacggcccc ggggtgacgg cgggagatca cgtcgaaggc gagcttgggc agccggacca
     1441 ggtacgagtc gcggatcgcg tacgccgatt ccgcgcgagg cgcgtggtcg atgagcgcct
     1501 cggcgccggc gagctgcccg tggccgcgtt cctcgctcgt cccgtcgggc gtggtcaggc
     1561 gcacgcgtcc gaacgagacg acgaacgcgt cgtcggaggg cgcgcccgcg tcgtagagct
     1621 tctcgccggc cggcagcgcg acccactcga ccatgctgcc gatcgctgcg cgcgttcgct
     1681 cgtccagcgg gccgaagagc gcagccagat cggtcgcgag gtgcttgtcg agcagccgcc
     1741 ggtgcgcctc ctccgcgagc cgctcctcga gctcggggta gtcgtgcagc agctgcgcga
     1801 agcgggtcgg agtgaaccgc gcgacgcgcg atttgatctc ggcgcgcacg gtcgcggcgc
     1861 gcttcgcgcc ggtcatgaac gcgatctcgc cgatcacgtc gcccggcgac aggtgcgaca
     1921 gcacctcacg atcgccgtcg acctcgacgc gcaggcggcc gcccagcacc acgtagacgt
     1981 cgcccccgtc cgagccttgc ttgatcagcg tgcggccgcg cgcaacctcg accagacgcg
     2041 cttgcgaggc gatcgcgcgc agatgctccg gcggaaggct gctgaacgag cgtgaggcgg
     2101 ccagcgaccg caccacaccc ggcgaaactc gtccatccgg catacggggc tccttccgga
     2161 ctattcgggc cgaatcctag cgcccgggcg gctaggcaag gcaagcgggc tcagcccggg
     2221 aagaccacgg catagaccgg tcccgccggc gccgacgcat tgccggcgag gtcgtccgcg
     2281 acggcgtgaa ccgtggccac gcccggcggc agcgacacgc tcacccgcca ccacacgctc
     2341 gcggcggcgc actcgggaca gtcggcgacg acctcggaaa gaagccggcc gatcgcgtcg
     2401 gaggagcgaa tgcggattcg cgccaccgcc acgtcgtcgg acgcgtcgcc gaacaggaac
     2461 acgcggccgg cgccgccgct gacgagaccc gaatggatcg tgacctcggg cggtgtcccg
     2521 tccggcgctc cgccgacggt gaacgtgcgc gtcaccgacc ctccttgcga cccgtccgcg
     2581 cccaacgcga gcgccgtcac ggtgtgcgtt ccgcccgcca tggaacgcac gatcgaccag
     2641 gcgccattct gtgcggcgag cacctcgccc aacgtcgctc cgcctccggt caagcgaacg
     2701 atgacccagg gctcggccgt tcccgaaaat gtcacgctgc ccgccgggac ggtcgcgccg
     2761 ggtgcgggct ccgcgaccac cggtgccgcc ggcggcgccg tgcgatcggt aagcaccccg
     2821 cacatcccgg tgcagatccc gctcgagctg accgtggagt gcggcgtgca gcgatacagc
     2881 acctctccgc cggtgtacgc gcggctgtag ggcggactgg agggactcat catgtcggag
     2941 gcgaagctca ttgccccggc ataggtcacg acgtcgtgtg cgccggtgcg cacggtccag
     3001 cgcacggtgt ctccttgaac gggcctggcc acgtccggag cgaagaagtt cgatcgcaca
     3061 tcgatgtcga tcgtagcgct cgcccgcgcc caagagcccg gcagcagcgc ggccagcagg
     3121 cagacgacaa gcgcgacagg cttcacgaga cggccgcctc cgcggcgtcg taggcatccc
     3181 aggccgcgca gcgcgcgtcc ggcgcgatgc gcgagaacgt gaagccgacc gcgcgtgcca
     3241 tccggatctg cgcgcacatg acgtgatcgc cgatcgtggg cgagacgttg aagatcacgt
     3301 tgtcgttgat cgccacctgc gaggcgaccg cgggccaggc ctcgacgggc gcgatcagat
     3361 agccgagctg gtcgtcggcg agcgcgagcg tccagtggcg ctgcggcggc acgttggtgg
     3421 cctcggccac gccggcggcg atgttcggat acggctcgcc gggtgcgccg ctgatcagca
     3481 gatcgccgat gcgaagcgcc gacacgatgg tgccgaccgt cgttccggtt agccacgggc
     3541 tctgttgcga gcgctggatc ggcgcgccga ccgcgtcgcc cttgaacagc agcgagccca
     3601 cgatcgggtt ctcgacgttg tggcggatca tggtctgcgc gccggcgacc gggccctgca
     3661 cctcgggcgc gccggtcatg gcctgctcca cgtggtacat cagcatcgtc gtgtacgact
     3721 tcacgcggtc cgcgatctcg tagccgggct tgcgcttatc ggtgaacgag cagcgcggcc
     3781 ggcacggctg cgttcgcccg ttcgtgcctt gataggcgat gccggtgccg ccgtagcgct
     3841 gcgtcagcgc ctcggacatg aactgcggcc agtcgccgtg gaccccggcg ccgccgccga
     3901 gcgtcgcatg cgccgcgtac gacatcgccg tcgcgatcac cgatccgttg ccggcgtcgc
     3961 gcgcttgcag cacgcgaacc cacgagtcct tcgactcgag gaacggatcg cacaccgtgt
     4021 tggggaagtt cgagtccggg ctctggttga gcgcctcggt gcaggtctgg ttgtagatca
     4081 ggtcgggtgc atccgaggcg cccgcgacga tgaccgcccc gcgccgagcc tggtaggcgg
     4141 cgacgacggc gtcgaccgtc cgggacgcga tgtgctccag gtagtcgctc gggatgaagc
     4201 cccacacgcc gatcgcgtcg ggcccggcat gggtgtgatc gttggagatc gtcacgttcg
     4261 cgggcggaag cgccggaacg gcggcatgaa ttcgctgcgc gatcgcgtcc agcccggact
     4321 cagggtcgtt ctggtaggca gcgaacatgc cttgggtctc gacgctgacc atcgcgacgg
     4381 caacgcccgt ggcggcgtct tcgaccacca gtgcgcgcgc ggcgatgtgg ttgccttgga
     4441 acgagctccg gccgccgcgg cccagcgcct ggctgaccgg cgacgagccg tcgccgaagc
     4501 cgtagccgcc ggtgttgatg gttccccagc cgatcgggct gatgtcgcgc acggccgcgc
     4561 ccacgcgata cggcgcggcg acctgcgagc gggcgggcag gcacgcggcg agcagcgctg
     4621 cgagcagcgc gggtgcgacg aggatgcggc gcatggacga atctcccggg tcgagggtca
     4681 gaccgatcac ttcgccgcgg agccgtctcg gtccttccgc cgggatcgtc cggcatcaag
     4741 cggcaggaaa ggaaccggac ggggccgcgt cgaaagggcg atgacatgag tcggatcgcg
     4801 ctcgtccttg ccgccgccgc cttggccgcc gcctcgacga ccgcgcgcgc gtccgccccg
     4861 cacgtagtca ccgcc
//
LOCUS       DATLFO010000190        18246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_63258, whole genome shotgun sequence.
ACCESSION   DATLFO010000190 DATLFO010000000
VERSION     DATLFO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63258"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VM841_07535"
     CDS             complement(<1..589)
                     /locus_tag="VM841_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL90069.1"
                     /translation="MTWKYTGEHAGAFTGMRPRARPHASPAGWARSGPAVVVRAALRA
                     VLLFPIVRLFCRRRIVEGAHRIPGGPVIFVANHAGHADTPLILRSLPRVQRNRLAPAA
                     AADYFFRTRLRGALTTLIVGAFPFPRCGTAGLDRADRLMRDGWSVLLFPEGTRSPDGR
                     IHEFKRGAARLAARRGATIVPVGIAGSREVLGKGAV"
     gene            complement(586..1482)
                     /locus_tag="VM841_07540"
     CDS             complement(586..1482)
                     /locus_tag="VM841_07540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVL90070.1"
                     /translation="MSSLAIESAARMSLALGGALGLIAFAERRHLKEMTRRVLFVRWR
                     TWAITAPIFGGAVMGGRWAAVAFVEVLTIVGLREYARIVSLPRPYRVVLYAAGIASAP
                     VAALSLTVWRGMPPILLIAATIPPLLMQDITRGVRDLAYAALGFAYIPWLLAYFILIL
                     EHVDGGAAILLALGASVAASDVGAFTVGKLAGRHAMAPRLSPNKTWEGAIGNLAGACA
                     GFALMSFAIPGTLNPVVRATLPIAVAFASIWGDLLESLIKRQFGVKDAGSWLPGFGGL
                     LDRIDSLLVVLPVSYTVLVIWG"
     gene            complement(1475..2071)
                     /locus_tag="VM841_07545"
     CDS             complement(1475..2071)
                     /locus_tag="VM841_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVL90071.1"
                     /translation="MDGLYAIKPWFVRRLRGVEDTLVSLRVSADALTWAAVGAAAGAG
                     VAIVAGTGAPLLWLAVAPLALTRIALNALDGSVARRTGTARPSGALLNEASDRIADAL
                     FLVPLAWVAGPALALAALAATLLASLAGVLSQALTGTRDHGGPMGKADRMLVLGVASL
                     VAPMQPRAWTVAAIAVASGALITAAARVTRIRRRLADV"
     gene            complement(2298..3143)
                     /locus_tag="VM841_07550"
     CDS             complement(2298..3143)
                     /locus_tag="VM841_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90072.1"
                     /translation="MLAMLGAACGKADVRSPGSDGRSAPRGADELILRVFTEGGFVPV
                     EFNLRNLPEFTLTGDGRVFSIGAQIEIYPPPALPPLLVRPIESDAVAKIIAEAKAAGL
                     DGPDKEYRYDLVADAGTTVFTFVDDDGVTHTIRAYALGFEADGGDAPPGQTAEEREAR
                     AKLAKLREKLLDLSSWLPRESLGEDKVYEIEKLRVFAKPYETPNPDEGQQVPKAWPLG
                     SDLATFGEAGAAGYRCGVLEGADLEKALKEFQASNTRTPWTSNGKQYELILRPLLPDE
                     SGCPK"
     gene            complement(3250..3915)
                     /locus_tag="VM841_07555"
     CDS             complement(3250..3915)
                     /locus_tag="VM841_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90073.1"
                     /translation="MSDAIRVVVADDHPVWRDGVAADLAKAGTIEVVGQAGDGGEVID
                     VVRDTMPDVVLMDLNMPVTGGAEATRVIHDESPHVKVLILSASAEESDVLEAVAAGAS
                     GYLLKSATGDELRDAIERVARGEPVFTPSLAGLVLGEFRRMASGGKPAAADEPKLTPR
                     ETEVLTYVAKGYTYREIGEKLFISVKTVQNHVQNILSKLHLSRRYELMRYAIRKGLDK
                     APE"
     gene            complement(3912..5105)
                     /locus_tag="VM841_07560"
     CDS             complement(3912..5105)
                     /locus_tag="VM841_07560"
                     /inference="COORDINATES: protein motif:HMM:NF039922.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90074.1"
                     /translation="MERSEARLNEADRARATLPLGVLAFRWASLAWMGALAATSSDFR
                     RPGLAWLAFGAAVAWTAIVSARVLREPDPAALWFDLGLACTLIVLSGFVVPQGAVVEG
                     RPFFATAYPAAAVLAWAVAKGLGGGLAAGAAAGLALILSRPANGIALGELTTAQWQTL
                     ANGIVTYALAGGAVGLVSRLIDRARTELKAALAERMREAQRAARLAEREALGRQIHDS
                     VLQSLALVHKKGKELAREGPVDAGEVRSLAELAGAQERALRSLIVRADVEPPQGTASL
                     RDHLEALGLSYAGTIAVTVSSAGAAYLPRAHVDEIVAAAKQAIDNAVEHAGPKAKVTV
                     FVDERDDATEVTVRDDGRGFDIAAANGYGLRSMRGRIQDIGGRFAISSEPGGGTEIEM
                     TVPKT"
     gene            complement(5081..6256)
                     /gene="mazG"
                     /locus_tag="VM841_07565"
     CDS             complement(5081..6256)
                     /gene="mazG"
                     /locus_tag="VM841_07565"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HVL90075.1"
                     /translation="MARLLLVRPGAGAPDLCSLEVWQALTGRPVLCDPNDELGDRLAQ
                     EGFPVERIDLAQSIHAATEPAPAGDKPKPRLNLLGHQHGAADPRLSALAARLAGEAAA
                     RGEVAYILPADAAPVVRAVMERAMQGGLEVEVVVGRVPRGHLLLDLVKVMARLRSPGG
                     CPWDAEQTHQTLAKHLLDEAYELLHAIEEGTSEDIAEELGDVLLQVVFHAQMGHDAQT
                     FDIDDVADTLIKKLVNRHPHVFGDVTVEGADEVVANWDVIKEKEKGRSGVHDDIPESL
                     PALAWAAKLQRRAGKAGFDWDDAHGPMDKVNEELRELGAAATDAEREHELGDLLLAVV
                     ALSRHLDVDAETALRRAGRRFKARVARIEEAVKAQGKTLRDLSERELIDLWNEAKRG"
     gene            complement(6706..7143)
                     /locus_tag="VM841_07570"
     CDS             complement(6706..7143)
                     /locus_tag="VM841_07570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL90076.1"
                     /translation="MSSEIERAIRERIHASEFAGWFGMEFVSAADGECEMRLELKPHH
                     LNPGGIIHGGVIATMLDAAIGLALRSARPLGSPHVTLTMSIQYIGMASEGTIVARGRS
                     LHAGRRAGHGEGELVTADGRMLAKGAATFMTVEPENLPRSPFA"
     gene            complement(7345..8517)
                     /locus_tag="VM841_07575"
     CDS             complement(7345..8517)
                     /locus_tag="VM841_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90077.1"
                     /translation="MSSELGAILLELTGRVIPDRIRGLDASLVLESPDEVWTMYFRGG
                     RMTIGAGAPDRADTVVRADTATLVGIQSGAVSGAGAFLDGLISVRGNLNLALRAESLF
                     EPLVKRPITAPRDGIALAGDHEVSIVEAGKGDPVILLHGLGATKVSFIPTMLALAPSY
                     RVIVPDLLGHGDTAKPRVSYSPQTFATFVLDLMDSLQIERAHLVGNSLGGRISLEVAM
                     TDPSRVRSMSLLCPAVAFLKRRWLAPIMRRMRPELAFLPQPIPHRAVVAGIRSLFVAP
                     ERLRRSWYDAAADEFLRVYRDRQARYALFDALRHIVTDEPMGDAGFWKRLERIEAPSM
                     FLWGDADPLVPSFYSTHVEQALPHATVEVITDCGHVPQFEYPAATHRKIKRFIAAA"
     gene            complement(8634..9023)
                     /locus_tag="VM841_07580"
     CDS             complement(8634..9023)
                     /locus_tag="VM841_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL90078.1"
                     /translation="MTAIREIMRTDVLEVEMAATLQEAAQAMRERKISSLAVTEKDRL
                     VGIMTERDIVTAVADGADSEVAHVRDYMTNSPVSAGAGTSVEEASQIMLEHGFRHLPV
                     VDDDQRLIGIVSLRDLMRAGVKVPTRE"
     gene            9111..9290
                     /locus_tag="VM841_07585"
     CDS             9111..9290
                     /locus_tag="VM841_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90079.1"
                     /translation="MLAALWMIVGALFGVACGFAAIPRNRSAVVWWFLGVLFGPVTLA
                     VLLRQGERAGPKAIL"
     gene            9287..10009
                     /locus_tag="VM841_07590"
     CDS             9287..10009
                     /locus_tag="VM841_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HVL90080.1"
                     /translation="MTGRLVGLLGSGEYLPWAAEVDRWLLDRASVGDGSVALLATAAA
                     PEGDAMFDKWSNMGLEHYRTMGVPAKVVPLKTREDASREAILSGIDTASLVFFSGGNP
                     AYLASTLRETPFWKRLMERVDEGAAVAGCSAGACLFGEVAPDPTKMGSPGEAFAPAGL
                     AVFPGFAFGAHWDVLDSYYPDLRGLSIRSMPPGCRMVGIDENTAMAADDGTWTVFGAG
                     GVHVYRSDEAITYTAGQTFTLP"
     gene            10006..10449
                     /locus_tag="VM841_07595"
     CDS             10006..10449
                     /locus_tag="VM841_07595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90081.1"
                     /translation="MIEIRPAAPGDAKTLTALAIRSKAHWGYDKTFMQACRPSLAIHA
                     TCVQPTFVAEVDGATAGFYQLEGSEIGAFFVDPPFIGRGVGRALWEHLMQEAQRRGLA
                     AVEILSDPNAEGFYRTMGAERAGEAPSDVFGPERMLPLMRAAIRR"
     gene            complement(10459..12810)
                     /locus_tag="VM841_07600"
     CDS             complement(10459..12810)
                     /locus_tag="VM841_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90082.1"
                     /translation="MRPPRIAALILAVCSAVAATAVAVPEAVPKVPVLTPETKTGSIV
                     RHAAPNQPPTAKVVDGNASDWTGEITRLGGTAVYSRGEYVYQDYLGDAWGADDGYDAG
                     RKAALDTAKSVSSRTYRVDALQQAAGAQFGAGRPVGASLRYGDTVPPPGVREQADIEE
                     VRIAADPEHLYVMVRTLAMTGSSPATAVAIFLDIEAGGPYSYPPAAGGVTSMAEHVFV
                     ASGNQITGLYRRGVPLISGCQLPCVPAPWTVASNPAGFDNVVEIAIRRDALDAAMPSS
                     LRIGVATGIAGPGGIAAMKAGDAKSDLINVAFRSEPARIWMDERQTFALRDGSIDEFF
                     ASIDLGTLEAGATETFTMRPGYYEAVYVNGGSPVNLEQTVNGYFQGPYQHYGVYLPDT
                     YRPGNVHPAMFWMHYRGGHAHDAAAWEPGVLHDFGDGGDAIVVTPSARGESSWYTGRG
                     MVDYLDVWDDAHARWSIDEDRTYLGGHSMGGWASWLLGLLFPDRWAASNPQDGLLVPG
                     LWAGAGPPSSPQNGADVEAEFLYPLLENARNLPYAILHGTADQLVPVTGAIAAGQRLH
                     DLGYRYRTYLYHAYEHFSAPIWDDWREMQSSMWSHRRDRNPERVTYKVSPALNHAVST
                     ISVPTGVDLGFEFNGAYWVSGMKVRAPGRAPSNLGTIDVTTYGRGLESRLLLPEASAG
                     VFGPYTMHGQRWVSNGRGDAANVFDMTATNLSDVTLDLARMSLSTAAGISGRVATDGL
                     MTLRLGGTWPSQPAVTGATVVSYDGATLVLRIPAAGVHHISVV"
     gene            complement(12898..14076)
                     /locus_tag="VM841_07605"
     CDS             complement(12898..14076)
                     /locus_tag="VM841_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HVL90083.1"
                     /translation="MEIPGKSRRTHALKPGDLAAIALAPGHPWPDLIREVWDAGAAVL
                     PIDHRLPPAAVQRLLAAARPAAIFDGSWWPQFESRPAGDEAAVAVATGGTTGGTTGEP
                     KIAVLSHRAVEAAVRTSAARLGAGPDDSWLACLPFGHIGGLLVVLRAVILGAPVTIHP
                     AFDPAALAAANASYTSLVPAMLARLLDAGADLSRFDAILLGGQATPAPLRERAEAAGA
                     RIVETYGQTESCGGVVYDGVPLDGTEMRLADGDMIELRGPTVMRGYLGAGGQPFTVDG
                     WLRTGDAGVIEQGRLIRAERAGDVIVSGGEKIYPSQVEDVLIRHPEIADAVVAARPDP
                     AFGSKVVAFIVCPDAVLSLEEVRDFVAAHLPRYAAPREVIVVPEVPRTPGGKIRRHLL
                     "
     gene            14128..14940
                     /gene="menB"
                     /locus_tag="VM841_07610"
     CDS             14128..14940
                     /gene="menB"
                     /locus_tag="VM841_07610"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HVL90084.1"
                     /translation="MDWIRSGDYQDISYETLDGIAKITICRPEVRNAFRPQTLFELQR
                     AFEVARDDATIGAIILTGQGPDAFCSGGDQRIRGDDGYVDAKGIGRLNVLDLQVQIRR
                     LPKPVIAMVAGYAIGGGHVLHLVCDMTIAAENARFGQTGPKVGSFDGGYGAGLLARTI
                     GLKRAKEIWFLCEQYDAQTALQWGLVNRVVPLEQLEEETVKVCRRILEMSPLALRMLK
                     AGFNADTDGLAGIQQLAGDATLLFYMSEEAQEGRDAFVEKRKPDFSKFPKRP"
     gene            14937..15863
                     /locus_tag="VM841_07615"
     CDS             14937..15863
                     /locus_tag="VM841_07615"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HVL90085.1"
                     /translation="MNEAAGARANAPSGAGLWLAGARPRTLGASVAPVIAGVAAAPSI
                     SWLRAAGALVVAVALQVGVNYANDYSDGVRGVDAQRVGPVRLTASGLASPAAVRRAAL
                     LSFLVAGLTGLALAIAVDLRLLAVGAAALLAAAAYSGGPKPYAGLGLGEVFVFVFFGP
                     VATAGTAYVTWGAGGIGSLPSVVWWGGAAIGLLGVAILLANNIRDIPTDAAAGKRTLP
                     VRLGDAEARALYRGVVVAALATVVVAVVLDDVPPLAMTALAAAPFAVPPLRAVGSARG
                     PAMVPVLLGTAALNLAFGMFLAAGLTIHRVTS"
     gene            15860..17542
                     /gene="menD"
                     /locus_tag="VM841_07620"
     CDS             15860..17542
                     /gene="menD"
                     /locus_tag="VM841_07620"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HVL90086.1"
                     /translation="MSGHAEISLACASLLADELARGGVEHVCVSPGARSTPLALAVAR
                     HPRLRVHVILDERSAAFFGLGIAKATRRPVALVCTSGTAAANWHPAIVEARYSQTPLI
                     ALSADRPPELRDTGANQTIDQRHLFASAPLWFFEMPVPAIEHDTERVWRSAGARAVAT
                     ASGPPAGPVHLNLPLREPLVASGDVPDLGAGRDGGAPWERHSPAAMLPSPGEVDDLAR
                     VIDATDDGMIVCGALDADAQEIMDLSERAGWPLVAEPLSGLRHGIALSAPQLILDRIE
                     PSVVLYTGAPPTSRATRALLDRTPRVLVVDAARWNDPDHRSSWTVRADAALLASALSA
                     VVKPREDRTWMQRWHDADASARAAADALMDSWDEPFEGRIARDVAAAVPDGGVLAIGS
                     SLPVRDLDSYMRPREDLWVIGNRGASGIDGFISTVLGIAQGGDPTVGLMGDLTFLHDA
                     GSLAWLAPRAKPAVLVVEDNGGGAIFDLLPQGGVREADDLFVTPHAIPIEPLVRAAGC
                     GYERVTRADAVVPAVAKAGDAGVTTIVHVACDRAAGPQRRAAIAAAVADALR"
     gene            complement(17539..>18246)
                     /locus_tag="VM841_07625"
     CDS             complement(17539..>18246)
                     /locus_tag="VM841_07625"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HVL90087.1"
                     /translation="RTLMLCFDGAPDVRSLLASLRDADPHAYTFAFGEMIGATPEQLV
                     ERRGAVVRCGPLAGTAPRGATPGADRTAAATLLESSKDHAEHAFVVRAIRDALEPRCE
                     TLTVEDPPSLLETSRVWHLRTRIDGRLRAPAPDALALAAALHPTPAVGGTPAEAALQA
                     IRDLEPVERGFYAGLIGWTDAAGDGEWAIQLRCGHVRGATMRLYAGAGIVAGSDPDAE
                     LAETDAKLAVMRAALAR"
BASE COUNT         2605 a         6222 c         6770 g         2649 t
ORIGIN      
        1 tgaccgctcc tttgccgagc acctcacgcg atccggcgat cccgaccgga acgatggtcg
       61 ctccgcggcg agcggcgagg cgggccgcgc cgcgcttgaa ctcgtggatg cggccgtcgg
      121 gggagcgcgt tccttcgggg aacagaagga ccgaccatcc gtcgcgcatc aaccggtcgg
      181 cgcgatcgag cccggcggtg ccgcaacgcg ggaaggggaa cgcgcccacg atgagcgtgg
      241 tgagggctcc gcgaagacgg gtgcggaaaa agtagtcggc cgcggcggcc ggcgcgaggc
      301 ggtttcgctg cacgcgcggc agcgaccgca ggatcagcgg cgtgtcggca tgcccggcgt
      361 ggttggcgac gaagatcacc ggcccgccgg ggatgcggtg cgcgccttcg acgatgcggc
      421 gccggcagaa caggcgaacg atcgggaaca gcaatacggc gcggagcgcc gcccgcacca
      481 ccacggccgg gccgctgcgg gcccatccgg ccggcgatgc gtgcggtctc gcgcgcggcc
      541 gcatacccgt gaacgcgccc gcgtgctcgc cggtgtattt ccaggtcatc cccagatcac
      601 cagcacggtg taggagacgg gcaggaccac gagcagcgag tcgatgcggt ccagcagccc
      661 gccgaagccg ggcagccacg agcctgcgtc cttgacgccg aactgccgct tgatcagcga
      721 ctcgagcagg tcgccccaga tgctcgcgaa ggcgacggcg atcggaagcg tcgcgcgcac
      781 cacggggttg agcgtgccgg ggatggcgaa gctcatcagc gcgaagccgg cgcaggcacc
      841 ggcgaggttt ccgatcgcgc cctcccaggt cttgttcggg ctgagcctgg gtgccatcgc
      901 gtgacggccg gcgagctttc cgacggtgaa ggcccccacg tcgctcgcgg cgaccgacgc
      961 gccgagcgcg agcaggatgg ccgctccccc gtccacgtgc tccaggatca agatgaagta
     1021 cgcgagcagc cacgggatgt aggcgaagcc gagcgcggcg tacgcgaggt cgcgcacgcc
     1081 gcgcgtgatg tcctgcatca gcagcggcgg gatggtcgcg gcgatcagaa ggatcggcgg
     1141 catgccgcgc cagacggtga gcgagagcgc ggcgacgggc gcgctggcga tgccggccgc
     1201 gtagagcaca acgcggtacg gccggggaag cgacacgatg cgcgcgtact cgcgcagccc
     1261 gacgatggtg agcacctcga cgaaggcgac tgcggcccag cggccaccca tcaccgcgcc
     1321 gccgaagatc ggggcggtga tcgcccaggt gcgccagcgc acgaacagca cgcgacgcgt
     1381 catctccttg aggtggcgcc gctccgcgaa ggcaatcagg ccgagcgctc cgccgagcgc
     1441 gagcgacatg cgtgcggcgc tctcgatggc gaggctagac atcggcgagc ctccggcgga
     1501 tgcgtgtcac gcgagcggcg gcggtgatca gcgcgccgga cgcgacggcg atcgcggcga
     1561 cggtccacgc ccgcggctgc atcggggcga cgagcgaggc gacgccgagc accagcatgc
     1621 ggtccgcttt gcccatcggg ccgccgtgat cgcgcgttcc ggtcagcgcc tgcgacaaca
     1681 cgccggcaag cgaggcgagg agcgtcgcag cgagcgcggc cagcgcaagc gcggggccgg
     1741 cgacccacgc cagcggtacg aggaagagcg cgtcggcgat gcggtcggaa gcttcgttca
     1801 gcagcgcgcc ggacggccgc gcggttcccg tccggcgcgc gaccgatccg tccagggcgt
     1861 tgagcgcgat ccgggtgagc gccaagggtg cgacggcgag ccagagcagc ggcgcgccgg
     1921 taccggcgac gatcgccacg cccgcgccgg ccgccgcacc gaccgccgcc cacgtcagcg
     1981 cgtccgcgga gacgcgcaac gacacgagtg tgtcctcgac gccgcggagc cgccgcacga
     2041 accagggctt gatcgcatac agaccgtcca tgccttcgat gatcggacgc gcgcgccttc
     2101 acgcctagac ggaggcgaac tcattcgcga tgccggtccc gctcaatcgc ctctgggtag
     2161 ttccggcacg ggcggttccg agccggcgcg gccgtgccac ctcgccacgc cgcaaagacg
     2221 cgaaatccct ccggctcgtg gtcggccgga gggattcgcc aggcagcgcg ccggcgtccc
     2281 cggggggtgt gtcggcgtta cttcgggcag cccgactcgt ccgggagcag aggacgaagg
     2341 atcagttcgt actgcttgcc gttgctggtc cacggcgtcc tcgtgttcga agcctggaac
     2401 tctttgagtg ccttctcgag gtcggctccc tcgagcacgc cgcagcggta cccggcggcg
     2461 ccggcctcgc cgaaggtggc gagatcggag ccgagcggcc acgccttcgg aacctgctgg
     2521 ccctcgtcgg ggttgggagt ctcgtacggc ttggcgaaga cgcgcagctt ctcgatctcg
     2581 tagaccttgt cctcgccgag cgactcgcgc ggcagccagg acgaaaggtc gagcagcttc
     2641 tcgcgcagct tcgcgagctt ggcccgcgcc tcgcgctcct cggcggtctg cccgggaggg
     2701 gcgtctccgc cgtcggcctc gaagccgagc gcgtaggcgc ggatcgtgtg agtgacaccg
     2761 tcgtcgtcga cgaacgtgaa caccgtggtg ccggcgtccg cgacgaggtc gtagcgatac
     2821 tccttgtccg ggccgtccag gccggcggcc ttggcctcgg cgatgatctt ggcgaccgcg
     2881 tcggactcga tcggccggac cagcagcggc ggcagcgccg gcggcgggta gatctcgatc
     2941 tgcgctccga tcgagaagac ccgtccgtcg ccggtgagcg tgaactcggg caaattccgg
     3001 aggttgaatt ccaccgggac gaagccgcct tcggtgaaca ctcgaaggat gagctcgtcg
     3061 gcccctcggg gggccgagcg tccgtcggat ccgggtgatc tcacgtccgc cttcccgcag
     3121 gctgcgccca gcatcgcgag catgagcagt cctgctaccg cttgccaccg tcgcatggtc
     3181 gttctccccg ttctgtctga agcgatcgat gcaccttgga cggcgacgag gcgcgccggg
     3241 ttcccgcaat tattcagggg ccttgtcgag gccttttcgg atcgcgtacc gcatcagctc
     3301 gtagcggcgg gacagatgca gcttggacag gatgttctgg acgtggttct ggacggtctt
     3361 gacgctgatg aagagctttt cgccgatctc gcggtaggtg tagcccttgg cgacgtaggt
     3421 caggacctcg gtctcgcgcg gggtgagttt gggctcgtcg gccgcggccg gcttgccgcc
     3481 ggaggccatg cgccggaact cgccgagcac caggccggcc agcgaggggg tgaagacggg
     3541 ctcgccgcgc gcgacgcgct cgatcgcgtc gcggagctcg tcaccggtgg cgctcttgag
     3601 caggtagccg ctcgcgccgg cggcaaccgc ctcgagcacg tcggactcct cggcggaggc
     3661 gctcaggatc aagaccttca cgtgcgggga ttcgtcgtga atgacgcgcg tggcctcggc
     3721 tccgccggtg accggcatgt tgaggtccat cagcaccacg tcgggcatgg tgtcgcggac
     3781 cacgtcgatg acctcgccgc cgtcgccggc ctggccgacg acctcgatgg tgccggcctt
     3841 ggcaaggtcg gccgcgacgc cgtcacgcca gacgggatga tcgtcggcca cgacgactcg
     3901 gatggcatcg ctcatgtttt gggcaccgtc atctcgatct cggttccgcc gccgggctcg
     3961 ctgctgatcg cgaaccggcc gccgatgtct tggatgcgcc cccgcatgct gcgcagaccg
     4021 tatccgttgg ccgcggcgat gtcgaagccc cggccgtcgt cgcgcacggt cacctcggtc
     4081 gcgtcgtcgc gctcgtcgac gaagacggtg accttggcct tcgggccggc gtgctcgacc
     4141 gcgttgtcga tcgcctgctt ggccgcggcg acgatctcgt ccacgtgcgc gcggggcaga
     4201 taggcggcgc cggcgctgga gacggtgacg gcgatcgttc cggcatacga caggccgagc
     4261 gcttcgaggt ggtcgcgcag gctcgcggtg ccctgcggcg gctcgacgtc ggcgcgcacg
     4321 atcagcgatc gaagcgcgcg ctcctgcgcg ccggccagct cggcgagcga gcggacctca
     4381 ccggcgtcca ccggcccttc gcgggcgagc tcctttcctt tcttgtggac cagcgcgagc
     4441 gactgcagga cgctgtcgtg gatctggcgg ccgagcgcct cgcgctcggc gagccgcgcg
     4501 gcgcgctgtg cttcgcgcat gcgttcggca agcgcggcct tgagctcggt tcgggcacgg
     4561 tcgatcagcc gggagaccag cccgaccgcg ccgccggcga gcgcataggt gacgatgccg
     4621 ttcgcgagcg tctgccactg cgcggtggtg agctcgccga gcgcgatgcc gttggcgggg
     4681 cgcgacagga tcagcgccag cccggcggcg gcgccggcgg cgaggccgcc gccgagaccc
     4741 ttggccacgg cccaggcgag cacggccgcg gccggatagg cggtggcgaa gaacggacgg
     4801 ccttcgacga ccgcgccctg tggcacgacg aagccggaga gcacgatcag cgtgcacgcc
     4861 agaccgaggt cgaaccagag cgcggcggga tcgggctcgc gcagcacgcg cgcggagacg
     4921 atcgcggtcc aggcgaccgc ggcgccgaag gcgagccagg caaggccggg gcggcgaaag
     4981 tcgctgctgg tggcggcgag cgcgcccatc cacgcgagcg acgcccagcg gaacgcgaga
     5041 acgccgagcg ggagcgtggc gcgcgcccgg tcggcttcgt tcagccgcgc ttcgcttcgt
     5101 tccacaggtc gatgagctct cgctcggaca ggtcgcggag cgtcttgccc tgcgccttca
     5161 cggcttcctc gatgcgcgcg acgcgagcct tgaagcggcg gccggcgcgg cgcagcgcgg
     5221 tctcggcgtc cacgtcgagg tggcgcgaga gcgcgacgac ggcgagcagc aggtcgccga
     5281 gctcgtgctc gcgctccgcg tcggtcgcgg cggccccgag ctcgcggagc tcctcgttga
     5341 ccttgtccat cggaccgtgc gcgtcgtccc agtcgaagcc ggccttgccg gcgcggcgct
     5401 gcagcttcgc ggcccaggcg agcgcgggca acgattcggg gatgtcgtcg tggacgccgc
     5461 tgcggccttt ctctttctct ttgatgacgt cccagttggc gacgacctcg tcggcgcctt
     5521 ccacggtgac gtcgccgaag acgtgcgggt gccggttgac gagcttcttg atgagcgtgt
     5581 cggcgacgtc gtcgatgtcg aaggtctgcg cgtcgtggcc catctgcgcg tgaaacacga
     5641 cctgcagcag cacatcgccg agctcttcgg cgatgtcctc gctcgtgcct tcctcgatgg
     5701 cgtgcagcag ctcgtaggcc tcgtcgagca gatgtttggc gagcgtctga tgcgtctgct
     5761 cggcgtccca cgggcagccg ccggggctgc gcagccgtgc catcaccttg accagatcga
     5821 gcagcaggtg accgcgcggg acgcggccga cgacgacctc gacctcgagc ccgccctgca
     5881 tcgcgcgctc catgaccgcg cgcacgaccg gggcggcatc ggcgggaagg atgtaggcga
     5941 cctcgccgcg cgcggcggcc tcgccggcga gccgggccgc gagcgcgctc aggcgcggat
     6001 ccgccgcgcc gtgctgatgg ccgagcaggt tgagccgcgg cttgggcttg tcgccggccg
     6061 gcgcgggctc ggtcgcggcg tggatgctct gcgcgagatc gatccgctcg accggaaagc
     6121 cttcctgcgc gaggcggtcg cccagctcgt cgttcgggtc gcagagcacc ggccggcccg
     6181 tcagcgcttg ccagacctcg agcgagcaca ggtccggcgc gccggcgccg ggtcgaacga
     6241 gcagcaatcg agccatcggc gcaagcgtac cggccggccg cggccggggc ggagatgcgt
     6301 accggcctgg ggcggagatg cgtcccggcc gggggctgcg gggcgggacc cggacggcct
     6361 cctgcgcgac ctcgtagcgg gccgggaagg gcgcaaaagg aaaacttcgt atgcgggcgg
     6421 cgaaggcacg ccccaaagtg gatgacagag gacgccggcg gtggcacact gtgctcacgc
     6481 gagaaaggag gtggtcccaa cttgtcgtta ccgcatagga cccgcgaggt ggctgaacgc
     6541 taacgggtgc gattcgggaa ccgcagggca ctcgctagcg gaaacccgtc agtccactga
     6601 gccgcgggaa gtccggagtg gtccaccggg ccgcctcctt aacggaaaag tcgttcccgc
     6661 ggaggaattc agagaggaga gccattcggc tctcctctcc tcttttcagg cgaagggcga
     6721 ccgcggcagg ttctcgggct cgacggtcat gaaggtcgcc gcgcctttgg cgagcatccg
     6781 gccgtcggca gtgaccagct ccccctcgcc gtgcccggcc cggcggccgg cgtgcaggga
     6841 gcggccgcgc gcgacgatgg tgccctcgct ggccatcccg atgtactgga tgctcatggt
     6901 gagcgtgacg tgcggcgagc cgagcggtcg cgccgaccgg agcgcgaggc cgatggcggc
     6961 gtcgagcatg gtggcgatga cgccgccgtg gatgattcct cccggattga gatgatgcgg
     7021 cttgagctcg agccgcatct cgcactcccc gtccgctgcc gagacgaact ccatcccgaa
     7081 ccagcccgcg aactccgacg catgaatgcg ttcgcggatg gctctttcga tctcgctgct
     7141 catggtgccg ggagaatacc gggttcgcgt cacctttcgg acgccgccgc gttagacggg
     7201 cggagggtcc gcatcctccg acgggtgcgc aacaggtgtg acccaccacg agcgcagccg
     7261 ccgcaccttg cggcccagcc gcttgcgggg tgatggtttt cgaaatgacc cggaccacga
     7321 gtccgggtct tttcgttttc cgcctcaggc ggcggcgatg aaccgcttga tcttgcggtg
     7381 cgtcgcggcc gggtactcga actgggggac gtgcccgcag tcggtaatga cctcgacggt
     7441 cgcgtgcggc agcgcctgct cgacatgcgt ggagtagaag gacggcacga gcggatcggc
     7501 gtcgccccac aagaacatgc tcggcgcctc gatgcgctcg aggcgcttcc agaagcccgc
     7561 gtcgcccatg ggctcgtcgg tgacgatgtg gcgcagcgcg tcgaagagcg cgtatcgcgc
     7621 ctggcgatcg cggtagacgc gcaggaactc gtcggcggcg gcgtcgtacc aggatcggcg
     7681 cagccgctcg ggcgcgacga acagcgaacg gatgccggcc acgaccgcgc gatgcgggat
     7741 cggctgcggc aggaacgcga gctcgggccg catgcgccgc atgatcggcg cgagccagcg
     7801 gcgcttgagg aacgcgacgg ccgggcacag cagcgacatg ctgcggacgc ggctcgggtc
     7861 ggtcatcgcg acttccagcg agatacggcc gccgagcgag tttccgacca gatgcgcccg
     7921 ctcgatctgc agcgaatcca tcaggtccag cacgaacgtc gcaaaggtct gaggcgaata
     7981 gctcacgcgc ggcttggcgg tgtcgccgtg gccgagcaaa tcgggaacga tcacgcgata
     8041 cgacggcgcg agcgcgagca tcgtcgggat gaacgacacc ttggtcgcgc cgaggccgtg
     8101 cagcaagatc accggatcgc ctttgccggc ctccacgatc gagacctcgt ggtcgccggc
     8161 gagcgcgatg ccgtcgcgcg gcgcggtgat gggacgcttg acgagcggct cgaacagcga
     8221 ctcggcccgc agcgcgaggt tcaggttgcc gcgcacgctg atcaagccgt ccaagaacgc
     8281 gcctgcgccg ctgaccgcac ccgattggat gccgacgagc gtcgcggtgt cggcccggac
     8341 gacggtgtcg gcccggtcag gcgcgcccgc gccgatggtc atgcggcccc cccggaagta
     8401 catggtccag acttcgtcgg ggctttcgag cacgagcgag gcgtcgagcc cgcggatccg
     8461 gtccgggatc acgcggcccg tcagctcgag caggatggcg ccgagttcgc tgctcacttc
     8521 gctccttgag gccatgtccg cttccttcga cgaagcaagc ggacgtcctg cgacgagttt
     8581 cgattgttcc accttagaac aacgaaccca tggctcccgg gatgcgcgag cgcctactcg
     8641 cgggtgggga ccttgacgcc ggcgcgcatc aggtcgcgca gcgagacaat gccgatgagc
     8701 cgctggtcgt cgtcgacgac gggcagatgg cggaagccgt gttcgagcat gatctggctg
     8761 gcctcctcga ccgaggtgcc cgcaccggcg gagacgggac tgttggtcat gtagtcgcgc
     8821 acgtgcgcaa cctcggagtc ggcgccgtcg gcgaccgcgg tgacgatgtc tcgctcggtc
     8881 atgatcccga cgagccggtc cttctcggtc accgccaggg acgagatctt ccgctcgcgc
     8941 atggcttgcg cggcctcctg cagcgtggcg gccatttcga cctcgagcac gtcggtgcgc
     9001 atgatctcgc gaatcgccgt catcgtccct cccgtaagtt ttgagcctcc gataatggta
     9061 gacgacccgg gcccggcgca agccggtccc ttcgtatact tcgaacgacg atgcttgcag
     9121 cgctttggat gatcgtgggc gccctgttcg gcgtcgcatg cggctttgcg gccattcccc
     9181 ggaaccggtc cgccgtggtc tggtggtttc tcggcgttct gttcggtccc gtgaccttgg
     9241 cggtgttgct ccggcaaggc gagcgggccg gcccgaaggc gatcctatga ccgggcggct
     9301 ggtgggcctg ctgggctcgg gggagtatct gccctgggcc gccgaggtgg accgctggct
     9361 gctggaccgg gcctcggtcg gcgacggaag cgtggcgctg ctggccaccg cggccgcacc
     9421 cgagggcgac gcgatgttcg acaagtggtc gaacatgggg ctcgagcatt accggacgat
     9481 gggcgtgccg gcgaaggtgg ttccgctcaa gacgcgcgaa gacgcctcgc gcgaggcgat
     9541 cctgagcggc atcgacaccg cgtcgctcgt gttcttctcg ggcggcaacc ccgcctatct
     9601 cgcgagcacg ctgcgggaga cgccgttctg gaaacggctg atggaacgcg tggacgaagg
     9661 cgcggcggtc gccggatgca gcgccggcgc gtgcttgttc ggcgaggtcg ccccggaccc
     9721 caccaagatg ggctcgcccg gcgaggcctt cgcgccggcg gggctggcgg tgtttccggg
     9781 cttcgcgttc ggcgcgcact gggacgtgct cgacagctac tacccggacc ttcggggcct
     9841 gtcgattcgg tcgatgccgc ccggctgccg gatggtgggc atcgacgaaa acacggcgat
     9901 ggccgccgac gacggaactt ggacggtgtt cggcgccggc ggcgtgcacg tctaccgaag
     9961 cgacgaggcg atcacctaca ccgccggtca gacgttcacg ctgccatgat cgagatccgg
    10021 ccggccgcgc cgggcgacgc caagacgctg accgcgctcg cgatccgctc gaaggcgcac
    10081 tggggatacg acaagacgtt catgcaggcg tgccggccgt cgctggcgat ccacgccacg
    10141 tgcgtgcagc cgacgttcgt cgccgaggtg gacggcgcga ccgcggggtt ctatcagctc
    10201 gaaggctcgg agatcggcgc gttcttcgtg gatccgccgt tcatcggtcg cggcgtcggc
    10261 cgtgcgctct gggagcacct gatgcaggaa gcgcaacgac gagggctcgc cgcggtcgag
    10321 atcctgtcgg atccgaacgc ggaaggcttc taccggacga tgggggccga acgcgccgga
    10381 gaggctccct ccgacgtgtt cggccccgag cgaatgctgc cgctgatgcg cgccgctata
    10441 cgacgctgat gcgcgccgct atacgacgct gatgtggtgc acgcccgcgg ccgggatccg
    10501 cagcacgagc gtcgcgccgt cgtaggacac gacggttgcg cccgtcacgg ccggctgcga
    10561 gggccacgtt ccgccgagcc ggagcgtcat cagcccgtcg gtagcgacgc ggccggagat
    10621 cccggcggct gtggacaggc tcatgcgggc aagatccagc gtcacgtcgg aaaggttcgt
    10681 cgcggtcatg tcgaagacgt ttgcggcgtc cccgcgtccg ttggacaccc agcgctggcc
    10741 gtgcatcgtg tacgggccga acacgccggc gctcgcctcg ggaagcaaga ggcgggattc
    10801 gagcccgcgg ccgtaggtgg tgacgtcgat cgtgccgagg ttggacggag cgcgtcccgg
    10861 cgcgcggacc ttcatgccgc tcacccagta ggcgccgttg aactcgaagc cgagatccac
    10921 gccggtcggc acgctgatcg tgctcaccgc gtggttgagc gcgggcgaga ccttgtaggt
    10981 gacgcgctcg gggttgcggt cgcgccggtg cgaccacatg gaggactgca tctcgcgcca
    11041 gtcgtcccag atcggtgcgg agaagtgctc gtaggcgtga tacaggtagg tgcggtagcg
    11101 atagccgaga tcgtgcaggc gctggcccgc cgcgatcgcg ccggtgacgg gaacgagctg
    11161 atcggcggtt ccgtgcagga tcgcgtaggg aaggttacgc gcgttctcga gcagcggata
    11221 caggaactcg gcttcgacgt ccgcaccgtt ctgcggcgag gacggcggac cggcccccgc
    11281 ccagaggccg ggaaccagaa gaccgtcctg cgggttcgac gcggcccagc ggtcggggaa
    11341 cagaagaccg agcagccacg aggcccagcc gcccatggag tgcccgccga gataggtgcg
    11401 atcctcgtcg atcgaccagc gcgcgtgcgc gtcgtcccag acgtcgagat agtcgaccat
    11461 gccgcggccg gtgtaccagg acgactcgcc gcgagcgcta ggcgtgacca cgatcgcgtc
    11521 gccgccgtcg ccgaagtcgt gcaggacgcc cggctcccac gcggccgcgt cgtgcgcgtg
    11581 gccgccgcga tagtgcatcc agaacatcgc gggatggacg ttgccgggac gataggtgtc
    11641 cggcaggtac acgccgtaat gctgatacgg gccttggaag tagccgttaa cggtttgctc
    11701 gaggttcacc ggcgagccac cgttgacgta gacggcctcg tagtagccgg gacgcatcgt
    11761 gaaggtctcg gtcgcgccgg cctccagcgt gccgagatcg atggacgcga agaactcgtc
    11821 gatgctgccg tcgcggagcg cgaaggtttg gcgctcgtcc atccagatgc gcgcgggctc
    11881 gctgcggaac gcgacgttga tcagatccga tttcgcgtcg cccgccttca tcgccgcgat
    11941 cccgcccgga ccggcgattc ccgtcgcgac accgatgcga agcgacgacg gcatcgccgc
    12001 gtcgagcgcg tcacgccgga tggcgatctc gacgacgttg tcgaacccgg ccgggttcga
    12061 cgcgacggtc cacggagccg gaacgcacgg aagctggcag cccgagatca gcggcacgcc
    12121 ccggcgatac aggccggtga tctggttgcc ggacgcgacg aagacgtgct cggccatgga
    12181 ggtcacgccg ccggcggccg gcggatacga gtagggcccg cccgcctcga tgtcgaggaa
    12241 aatcgcgacg gccgtcgccg gcgaggaccc cgtcatcgcg agcgtgcgaa ccatgacgta
    12301 gaggtgctcg gggtcggcgg cgatgcggac ctcttcgatg tcggcctgct cgcgcacgcc
    12361 ggggggcggg accgtgtcgc cgtagcgcag cgacgcgccg acgggccggc cggcgccgaa
    12421 ctgcgcgccg gccgcttgct ggagcgcatc cacgcgatag gtacgcgacg agacgctctt
    12481 cgcggtgtcg agcgccgctt tgcggcccgc gtcgtagccg tcgtcggcgc cccacgcatc
    12541 gccgagatag tcctggtaga cgtactcgcc gcgcgaatag acggcggtgc cgccgaggcg
    12601 cgtgatctcg ccggtccagt cgctcgcgtt gccgtccacg accttggcgg tgggcggctg
    12661 gttcggcgcc gcgtggcgca cgatcgatcc ggtcttggtc tcgggggtca ggacgggaac
    12721 cttcgggacc gcctcgggaa cggcgacggc ggtagctgct acggccgagc agacggccag
    12781 gatcagggcg gcgatgcggg gtgggcgcac gaggggtcct ttccggcttg cggagtcgtc
    12841 gccggaaggt tcgcggttcg ttttgcggaa tcctgccggt ccgttgagcc ggtccgtcta
    12901 cagcaggtgc cggcggatct tgccgccggg ggtgcggggc acctcgggca cgacgatgac
    12961 ctcgcgcggc gccgcgtagc gcggcaggtg cgcagcgacg aagtcgcgaa cctcctcgag
    13021 cgacagcacg gcgtcgggac agacgatgaa cgcgaccacc ttggagccga aggcgggatc
    13081 cggccgcgcc gccacgaccg cgtctgcgat ctcgggatgg cggatcagca cgtcctcgac
    13141 ctggctcggg tagatcttct cgccgccgct gacgatcacg tcgccggcgc gctcggcgcg
    13201 gatcaggcgg ccctgctcga tcacgccggc gtcgccggtt cgcagccagc cgtccactgt
    13261 gaacggctga ccgcccgcgc cgaggtagcc gcgcatgacg gtcggtccgc gaagctcgat
    13321 catgtcgccg tcggcgagcc gcatctcggt tccgtcgagc ggcacgccgt cgtagacgac
    13381 gccgccgcat gactcggtct ggccgtaggt ctcgacgatg cgcgcgccgg ccgcctcagc
    13441 gcgttcgcgc agcggcgcgg gcgtcgcctg cccgccgagc aggatcgcgt cgaagcgcga
    13501 cagatcggcg ccggcgtcga gcagccgcgc gagcatcgcc ggcacgagcg aggtgtagga
    13561 ggcattcgcg gccgcgaggg cggccggatc gaaggccgga tggatcgtga ccggagcgcc
    13621 gaggatcacc gcgcgcagca ccaccagcag cccgccgatg tggccgaacg gaagacacgc
    13681 cagccaggaa tcgtcgggtc ccgcgccgag tcgcgcggcg ctcgtgcgca cggcggcctc
    13741 gacggcccgg tgggaaagga ccgcgatctt gggctcgccg gtggtgccgc cggtggtgcc
    13801 gccggtcgcg accgcgaccg cggcctcgtc gccggccgga cgcgactcga actgcggcca
    13861 ccaggatccg tcgaagatcg ccgcgggacg cgcggccgcg agcaggcgct gaaccgccgc
    13921 cggaggaagc cggtggtcga tgggcaggac cgcggccccg gcatcccaga cctcgcggat
    13981 cagatcgggc cacggatggc cgggcgcgag cgcgatcgcg gccaggtcgc ccggcttcag
    14041 cgcgtgtgtg cgcctggatt ttcccgggat ttccatgtcg gctaggctaa ccgaaggttt
    14101 gagtaaacgc tctaggagtg aatggtcatg gattggatcc gctcggggga ctaccaagac
    14161 atcagctacg agacgctgga cgggatcgcc aagatcacca tctgccgtcc cgaggtccgc
    14221 aacgcgttcc gtccgcagac gctgttcgag cttcagcgcg cgttcgaggt cgcgcgcgac
    14281 gacgccacca tcggcgcgat catcctgacc gggcaaggtc cggatgcgtt ctgttcgggc
    14341 ggcgatcagc ggatccgcgg tgacgacggc tacgtcgacg ccaagggcat cggccggctg
    14401 aacgtgctcg acctgcaggt gcagatccgg cgtttgccca agccggtgat cgcgatggtc
    14461 gccggctacg cgatcggtgg cggccacgtg ctgcacctgg tgtgcgacat gaccatcgcc
    14521 gccgagaacg cacgcttcgg ccagaccggc ccgaaggtcg gctcgttcga cggcggctac
    14581 ggcgcgggtc tgctcgcgcg caccatcggc ttgaagcgcg cgaaggagat ctggttcctg
    14641 tgcgagcagt acgacgcaca gacggcgctg cagtgggggc tggtcaaccg ggtcgtgccg
    14701 ctcgagcagc tcgaggagga gacggtgaag gtgtgccggc ggatccttga gatgtcgccg
    14761 ctggccctgc gcatgctcaa ggcggggttc aacgccgaca ccgacgggct cgccggcatc
    14821 cagcagctcg ccggcgacgc gacgctgctg ttctacatga gcgaagaggc gcaggaaggc
    14881 cgcgacgcgt tcgtcgagaa gcgcaagccc gacttctcga agttcccgaa gcgtccgtga
    14941 acgaggcggc cggcgcgcgc gcgaacgcac cgagcggtgc ggggctgtgg ctcgccggcg
    15001 cgcggccgcg cacgctcggc gcgtcggtcg cgccggtgat cgcgggggtc gcggcggcgc
    15061 cgtcgatctc gtggctgcgc gccgccggcg cattggtcgt ggcggtcgcg ctgcaggtcg
    15121 gcgtcaacta cgccaacgac tactccgacg gcgtgcgcgg cgtggacgcg cagcgcgtgg
    15181 ggccggtgcg gctgaccgcg tcgggactcg cctcgcccgc ggcggtgcgc cgcgcggcgc
    15241 tgctgtcgtt cctcgtcgcg ggcctgaccg gcctggcgct ggcgatcgcg gtggatctgc
    15301 ggctgctcgc ggtcggcgcc gcggcgctgc tggcggccgc agcgtattcg ggcggaccga
    15361 agccgtacgc gggcttgggg ctgggcgagg tgttcgtgtt cgttttcttc gggccggtcg
    15421 cgaccgcggg aaccgcatac gtcacgtggg gcgcgggcgg catcggatcg cttccgtcgg
    15481 tcgtgtggtg gggcggcgcg gccatcgggc tgctcggcgt cgcgatcctg ctcgccaaca
    15541 acatccgcga catcccgacc gacgccgccg ccggcaagcg cacgctgccg gtgcggctcg
    15601 gtgacgccga ggcgcgcgcc ctgtaccgcg gcgtggtggt cgccgcgctc gcgaccgtgg
    15661 tggttgccgt ggtcctcgac gacgtgccgc cgcttgcgat gaccgcgctg gcggccgcgc
    15721 cgttcgcggt gccgccgctg cgcgccgtgg gatcggcccg cgggccggcg atggtgccgg
    15781 tgctgctggg aaccgcggcg ctgaacctcg cgttcggcat gttcctcgcg gcggggctga
    15841 cgatccaccg ggtgacctcg tgagcggcca cgccgagata tcgctcgcct gcgcgtcgct
    15901 gctggccgac gagctcgcgc gcggcggcgt cgagcacgtg tgcgtctcgc ccggcgcgcg
    15961 gtccacgccg ctggcgctcg cggtcgcgcg gcatccgcgg ctgcgcgtgc acgtgatcct
    16021 ggacgagcgg tcggccgcat tcttcggcct cggcatcgcg aaggcgaccc gccggccggt
    16081 cgcgctcgtg tgcacctcgg gaacggccgc ggcgaactgg catcccgcga tcgtcgaggc
    16141 acgctattcg cagacgccgc tgatcgcgct gtcggccgac cggccgccgg agctgcgcga
    16201 caccggcgcc aaccagacga tcgatcagcg tcacctgttc gcgtcggcgc cgctgtggtt
    16261 cttcgaaatg cccgtgcccg cgatcgagca cgacaccgag cgggtgtggc gaagcgccgg
    16321 cgcgcgcgcc gtcgcgaccg cgtcgggacc gcccgccggt ccggtccacc tgaacctgcc
    16381 actgcgcgag ccgctcgtcg cgtccggcga cgtgccggac ctgggcgccg gccgcgacgg
    16441 cggcgccccg tgggagcgtc actcccccgc cgcgatgctg ccgtccccgg gcgaggtgga
    16501 cgatctggcg cgcgtgatcg acgcgaccga cgacggcatg atcgtgtgcg gcgccctgga
    16561 tgccgacgcc caagagatca tggacctgtc cgagcgcgcc ggctggccgc tggtggccga
    16621 gccgctgtcg ggtctgcggc acggcatcgc gctctcggcg ccgcagctga tcctggaccg
    16681 gatcgagccc tcggtggtgc tgtacacggg tgcgccgccg acctcgcgcg cgacgcgcgc
    16741 gctgctggat cgcacgccac gggtgctcgt ggtggatgcc gcgcgatgga acgatccgga
    16801 tcaccggtcg tcgtggaccg ttcgcgcgga cgcggcgctg ctggcatcgg cgctgagcgc
    16861 ggtggtcaag ccgcgcgagg accggacctg gatgcagcgc tggcacgatg ccgacgcgag
    16921 cgcacgggcc gcggccgacg cactcatgga ttcctgggac gagccgttcg aaggccggat
    16981 cgcccgcgac gtcgcggccg ccgtgccgga cgggggcgtg ctcgcgatcg gatcgtcgct
    17041 tcccgttcgc gacctcgact cgtacatgcg gccgcgcgag gatctgtggg tgatcggcaa
    17101 ccgcggcgcg agcggcatcg acggcttcat ctcgacggtg ctcggcatcg cgcagggcgg
    17161 cgacccgacg gtcgggctga tgggcgacct gaccttcctg cacgacgccg gctcgctcgc
    17221 ctggctcgcg ccgcgcgcga agcccgcggt gctggtcgtc gaggacaacg gcggcggcgc
    17281 gatcttcgac ctgcttccgc aaggcggcgt gcgcgaggcc gacgacctgt tcgtcacccc
    17341 gcacgcgatc ccgatcgagc cgctcgttcg cgcggccgga tgcggctacg agcgggtgac
    17401 gcgcgcggac gcggtcgtcc cggccgtggc caaggcggga gacgcgggcg tcaccacgat
    17461 cgtgcacgtc gcctgcgatc gcgcggccgg gccgcaacgc cgcgccgcga tcgccgccgc
    17521 cgtcgcggac gcgctgcgtt aacgcgcgag cgcagcgcgc atcacggcaa gcttcgcgtc
    17581 ggtctcggcg agctcggcgt cgggatccga gccggcgacg atgccggcac ccgcgtagag
    17641 gcgcatcgtc gcgccgcgca cgtggccaca gcgaagctgg atcgcccact cgccgtcccc
    17701 ggcggcgtcg gtccagccga tcaggcccgc gtagaagccg cgctcgacag gctcgaggtc
    17761 gcggatcgcc tgcagcgccg cctccgcggg cgtcccaccc acggcgggcg tgggatgcag
    17821 cgcggccgcg agcgcgagcg cgtccggcgc cggagcgcgc agccggccgt cgatgcgcgt
    17881 gcgcagatgc cagacgcggc tggtctcgag cagcgacggc ggatcctcga cggtgagcgt
    17941 ctcgcaccgc ggctcgagcg cgtcgcggat cgcacgcacg acgaaggcat gctcggcgtg
    18001 gtccttcgac gattccagga gcgtcgcggc cgccgtgcga tcggccccgg gcgtggcgcc
    18061 gcgcggcgcg gtgcccgcga gcgggccgca tcgcacgacg gcgccgcgcc gctcgacgag
    18121 ctgctcgggc gtggcgccga tcatctcgcc gaacgcgaag gtgtaggcgt gcgggtcggc
    18181 gtcacgcagc gaggccagca gcgagcgcac gtcgggcgcg ccgtcgaagc agagcatgag
    18241 cgtgcg
//
LOCUS       DATLFO010000191        27989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_93910, whole genome shotgun sequence.
ACCESSION   DATLFO010000191 DATLFO010000000
VERSION     DATLFO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93910"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..298
                     /locus_tag="VM841_07630"
     tRNA            227..298
                     /locus_tag="VM841_07630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:260..262,aa:Arg,seq:cct)
     gene            complement(324..965)
                     /locus_tag="VM841_07635"
     CDS             complement(324..965)
                     /locus_tag="VM841_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90088.1"
                     /translation="MKARLLAALVAAAFALPAPSQADPVEITETSSNRVELLACSTWI
                     EPAGDEYEWTDVCAGDDDYDGDGVIERVAFVQQSMCDHDGSAPGGWCWTSGPSFFAEL
                     AAGDSFIAADRSSAWLHADVDGCRIDLDWTGGQSERSYDSEGAISPVLFPTPMEEGIA
                     AGYRRSGTSAKLRSATEQGSLCPAAGVSGAAWGSVIGEVTETDRETLVTIRLG"
     gene            complement(1246..2382)
                     /locus_tag="VM841_07640"
     CDS             complement(1246..2382)
                     /locus_tag="VM841_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HVL90089.1"
                     /translation="MDRRAFLKSAGLALAFAACSRAPMSPMPTVSGPHRRYGPLQAPD
                     ANGIRLPDGFTSRVIATSNDRVPGSRYTWHRAPDGGATFASPDDGWIYVSNSEVSRRG
                     GVGAVRFDAQGEIADAYRILDGTTRNCAGGPTPWGSWLSCEEFAQGRVWECDPQGERE
                     AEARPALGVFKHEAAAVDDRGKRLYLTEDESDGRFYRFTPTLYPSLEQGRLEAMRASE
                     DGSVEWIEIEDPSARRTPTRLQAPASTRFRGGEGCWFDDRTRTVFFTTKGDDRVWAYR
                     PDDERIEVLYDGRRQRGALSGVDNVVVTPAGDVLVAEDGGNMELVLITTDGIVEPLIR
                     VEGHHDSELAGPAFDPSGTRLYVSSQRGGHGDGITYEISGPFVP"
     gene            2479..2847
                     /gene="trxA"
                     /locus_tag="VM841_07645"
     CDS             2479..2847
                     /gene="trxA"
                     /locus_tag="VM841_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVL90090.1"
                     /translation="MPVIELDAATFRRHVLESTTPVVVDFYADWCGPCKQVAPVVEAL
                     AAEWEDEVTFAKLDIDASPEVAAAFNIRSIPAIILFEDGAPKAWSLGAKPGYLLEREL
                     GLAKRKRDKARGEKRSWWRR"
     gene            complement(2920..3459)
                     /locus_tag="VM841_07650"
     CDS             complement(2920..3459)
                     /locus_tag="VM841_07650"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVL90091.1"
                     /translation="MFADASAFLGWFEGVHRRTLRDVVLLPEAAERWLPPAPDPESGV
                     AWGVPDLVRHLCEGRLYFTGAFLNRGWIWDTWESELATRAEWSEALESSFAAVRDALE
                     GLPPEAMTCRIELIAAPDRTISAWRALMMMAEHEIAHRAQIGAYAGLNGWPVAQIFDR
                     TNEWVRSQRGDEAARRSDA"
     gene            complement(3471..3722)
                     /locus_tag="VM841_07655"
     CDS             complement(3471..3722)
                     /locus_tag="VM841_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90092.1"
                     /translation="MEKSILENLEEGDPVSVALRGGAGWVHGSMVWRRDGMMLIESAD
                     DAVRKETPFVLILLDEVSAVAIPSKVDPPGSSRSPGFNR"
     gene            complement(3735..6053)
                     /locus_tag="VM841_07660"
     CDS             complement(3735..6053)
                     /locus_tag="VM841_07660"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVL90093.1"
                     /translation="MRYLVTGGAGFLGSHLIERLLGDGDEVICVDNLLTGRREHVEMF
                     AGNPRYSFLEHNISLPLYVDKPVDVVMHLASPASPIDYLEHPIPTLKVGALGTHHTLG
                     IAKLHRATYFLASTSEVYGDPQIHPQPEGYWGHVNPIGPRGVYDEAKRYAEAMTMAYH
                     REHELDTRIVRIFNSILADEQVLYDDGTQLRRETIGELAARVGGQIDLEGYSVPAFDQ
                     DGAIRSSEASALVGHPPTGPCFVVRTRYGRSIRVTGDHSVFVQGPEGLPMARPVSQLS
                     TSDKIAVAARIEVPERERSHISTIEAWEAGGRDPWDLMVVAPGLGAIAWEKRFDLFGA
                     MAATGKWSGATKWRSAVWGMISGMRERNSVPLAGLRALGVPVPDEAWVLPRTAGRSTP
                     LPAHIEITDELLWFLGLYVAEGCWQERPTKSAFITLSCEEETLDRAAKILQRDLALHV
                     VRTPHTPQRSASMVVHSTLLLRVLEHLGFGAGRKRIPGWILGLPLSRLKWFLEGYREG
                     DGVHSGKMAEAIRHEFSTTSDELKDDLIVALARFGLLPSVGRYESTDKPRSGEKRFPF
                     WRLTLAHVAPWDMLEWDRGVEQVLQAVRTGDIVWAQVQAIEQVPATDLVYDFCVPGRE
                     NFWAGTGIMAHNTYGPRMRPTDGRAVSNFIVQALRNEPITVHGDGSQTRSFCYVSDLI
                     EGFVRLVKSGEHQPVNIGNPEEYRVIDLARMIVEIAESKSEITFMQRPVDDPNVRRPD
                     ITRAREVLGWEPAVPVREGLRRTIEYFRTLDL"
     gene            6092..6631
                     /locus_tag="VM841_07665"
     CDS             6092..6631
                     /locus_tag="VM841_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90094.1"
                     /translation="MALIEDLMPAWDVRKRYERLVRAPAGRTWAALQRYDISRNSSAT
                     TKALLRLRGLRPEAGTGVGAFAGSRFSVVGETPGAELVVAIAGKFWAIRESNALEPVE
                     DAAQVSAYDAPGRAVAVWSFEVAPRNGRSVLATETRVRCTDDRARRIFKAYWTVIEPF
                     SGMIRRDMLRTVAEMAEGS"
     gene            complement(6689..10303)
                     /locus_tag="VM841_07670"
     CDS             complement(6689..10303)
                     /locus_tag="VM841_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL90095.1"
                     /translation="MIDKVLGAVAFARVPRRGLKAVLVFAVVAASALVSQASLAAGPT
                     PGIVKVDTKDLNGDGYLDAVVVTFDTLMDQSVAAPNGFRVAGFQPGSPASQWLSAVQL
                     QVNLVPNLGTGGDTALRPEVSYAAASQKAKAIGTSGSELPDTQIPANDTTIVDAAGPV
                     LMSARAKDFGTPNVFAEAPDELQFNFSEPVRIEGATAAARIANLELAMKFSSLGVCNG
                     DAQDTNKPAGSTNFPSLKTGQTVDPIVEPASGERSARVRVRLVDPAAHNASSIKTPAI
                     PGGCGVGVDQTGASHITDGVAPAPNNAVHQDFGAVNRYRREIGIANTDLFYAQNGEPG
                     LSTQDLTPASGDGFVDAIAVMFEHNLDDATVTDALASKVTVRNGNEIATVSSVTTGAT
                     GDSTLLVHVTGVQWGGGVRPVVSFDGSGCTLKAHVPTGASFRACAGSFGGAAGVQALD
                     KVPPAVLGSAALDNDANGLIDTVRVNVTEAVASTATQAGWSVAGQPATGMTVNGATLH
                     LAITESPSLGTGATPAVSYNQSAGNTTDTAGASMKTQTFNAADGAAPRVVRATSLSSG
                     NNATTDRVTIEFSEPVAAGSLDAAAFTVDGVAASGFGSSALDPTPAATDNLVTITVAK
                     DGTGHVGLAFTGSIADLAGNENTQGFTMAAPEVADGAKPFVVSITTTPASPFGTGTVS
                     VSATFSEPLDTNVAPVVKFGDKDITPVSDANHTNGFRNAAPHIWDGTVTLAAEDCNQQ
                     NGCESSITLTGAKDTVGNAQVAATPVAVVADTVAPSAATGLASAAQQIDGAPSIGQNK
                     IGSKTRNLSVTASITAGQLHMVGQTTGGTAELLLDGQPFATPVRIAGIDQNATSITVT
                     TAFADAAALQSAIAEGPHTLTINLCDSASNCTVSEGLEVVADYSPVAVTLAGPHTGTF
                     AGGDQVCIQWTGDETGNDFTAAELRYSTDGGATYPHSIASGLARSGSLGCGVEGGGYL
                     WTVPELNTQALKVRISTLDAFGNSAAAQSASNMTVESITETAITLSSSPNSLVYGAGT
                     YMVGFLTGNDAGVAGKTVTIQRRIWGTDVFESFVTATTGADGRFAYWFKPDRHAEYRA
                     IFNGDGPYLASTSAPQQVWVAVAVASRVNSTSLQRGQWFHINGWVLPAHPGEEVWFQA
                     YLGGKWVTLAKQKLGSRNYYQFWYRSNSAVNLLFRVAFPTQDRDHAWNISQRYMVTWR
                     "
     gene            complement(10622..11908)
                     /locus_tag="VM841_07675"
     CDS             complement(10622..11908)
                     /locus_tag="VM841_07675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVL90096.1"
                     /translation="MKVAIVGTGYVGLVTGSCMADMGHDVICVDVVEEKVAKLRSGQI
                     PIHEPGLPDIVERNLAAGRLSFTTSLAEAVRPAQFVFICVPTPPAEDGSADLSHVEKV
                     ATDVAPLISTYKIIVNKSTIPVGSSMRVERIVRERLAPGVETDVASNPEFLREGSAVF
                     DFMHPDRIVIGTRSERAAGLLTELYRSLGAPLIVTDPETAEMIKYASNAFLATKISFI
                     NAISNICDAVGADVKEVALGMGYDARIGFEFLKPGPGFGGSCFPKDIKALIRVAEQNG
                     YDFHLLRGVLDVNEQQHRIIANKVEKVTGGVSGKTIAAWGLAFKPNTDDVRDSPAIAV
                     LRELMDRGATIRGYDPVVAESVYDQLGIERAPGPMEAADGADAVLLLTEWNEFRWLDF
                     ERLRDSMRAPAIVDARNLLDPHTLRQLGFSYEGVGR"
     gene            complement(11925..12878)
                     /locus_tag="VM841_07680"
     CDS             complement(11925..12878)
                     /locus_tag="VM841_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005548569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVL90097.1"
                     /translation="MRVLITGVTGFVGSHLAEYCLEQGHEVAGTFRWRSRDENLGIVK
                     GKVALHDADLRDPVGVRKVIQAFQPERVFHLAAQSYVHASWASPAETLSTNIISQVNL
                     LEAIREAGLTDCRFQVAGSSEEYGMVHPDETPIKESNPLRPLSPYAVSKVAQDLMGYQ
                     YHESYGMYIVRTRAFNHEGPRRGEVFVTSNFAKQIAEIEKGKKEPVIHVGNLDAYRDY
                     TDVRDIIRAYMLALDKCEPGEVYNIGSGNAWQIRDMLSTLLDYSDVEVEIRTDPERMR
                     PSDVPRLECDATKFKKVTGWEPQIPFETTLKDTLDYWRERV"
     gene            12986..14266
                     /gene="wecB"
                     /locus_tag="VM841_07685"
     CDS             12986..14266
                     /gene="wecB"
                     /locus_tag="VM841_07685"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HVL90098.1"
                     /translation="MKIANIVGARPQFIKAAAVCAAARADGRIQDYLIHTGQHYDADM
                     SDIFFTQLGLPRPDVELGIGSGPHGQQTGQMMMALEGTLSDLSPDVTLVYGDTNTTLA
                     GALTAQRLGIPVAHVEAGLRSFNRTMPEEINRIVTDRVSAILFCPTKTSVANLEREGI
                     TKGVNLVGDVMIDVMRGSVTWARMGILGELGVRTGAYYLMTLHRAGNTDDPERLATIL
                     KAVAKLPYPVVFPAHPRTRAKMSAAGLEPEGNLRMIEPVGYMESLALQMHARAILTDS
                     GGVQKEAYWLGVPCVTLREDTEWVETIEVGWNILAGADPELIVAGVERFPPEDRPPLY
                     GDGSTARQIVERLAAGPPWDAPMPKPESKASRPRPAKPTPKRAVAAAKPAPKRKAASA
                     DNKAAAKAPAKKKTTTTKKKASVKKTAAKKKAAE"
     gene            14263..14817
                     /locus_tag="VM841_07690"
     CDS             14263..14817
                     /locus_tag="VM841_07690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVL90099.1"
                     /translation="MSARIHPTATVDDGAIIGEGTSVWHQAHVRAGAVIGAGCMIGKN
                     VYVGADVHIGDHCKIQNNALIYEGVSLGDGVFIGPGAIIANDRRPRAVNPDGTIKGAG
                     DWTLAEARIEHGASIGAGAILIPPLIVGAWAMVAAGAVVARDVEPQSLVAGNPARHAG
                     WVCVCGARTQPDDKCGDCGRILEA"
     gene            14818..16092
                     /locus_tag="VM841_07695"
     CDS             14818..16092
                     /locus_tag="VM841_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HVL90100.1"
                     /translation="MSTKVAVVGLGKIGLPLSVQFALNGCDVIGCDVSEARVAEVTTA
                     TNPFPDEAHLDERLRAAVKRGQLRATTDTTAAVRDADVAILIVPLYAGPDHTPDVSIM
                     ESAADAVADGLHDGMLIVVETTMPVGATRRLAQRMEQRSGQTPGSGFFMAFSPERVYS
                     GRIFADLLAYPKLVGGLDAESGKRAAAFYRSVLPHVEVRELANAETAEMAKLAETIYR
                     DVNIALANELARYADGRGVDIGQVIAASNSQPFSHIHEPGVGVGGHCIPHYPWFVIAD
                     DPEATLMPAARAINDGQPGWVLDRVASEIGGLEGKRVLVLGLSYRAGVKEPTSSPGID
                     LVRLLPERGAAAYGHDPLFDDDEIAAFGATPATPEDLGEFDAVIVQAAHPEYGRIDWS
                     GCKPGCAVVDGRNTIDRASVEAGGARYLGIGR"
     gene            16089..17270
                     /locus_tag="VM841_07700"
     CDS             16089..17270
                     /locus_tag="VM841_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL90101.1"
                     /translation="MRRRRRVLIVTHSYYLRDTRPRRHAEALIDAGFEVDVLCCRDEG
                     EPQNERHRGVNIRRLPFRRERGGKVRYLFEYASFILVALLTVAAMHLRRRYKVVWILS
                     IPNTMVFTGLIPRLLGTPVVLDVRDPMPEFFFSKYGMSPGSPLSRALLTEERLSARFA
                     SHLVTVHRPLLDALLRTGVPRSRTTVVMNAPDLRLLDVPDRSLRDPDDRTVLYAGTIA
                     ARYGVDVALEAVALLHSEIPRIRLRLVGDGDLVPALQARARTLGIADRISFDGPVSLP
                     EIPAIVGQSWVGVQAHRLDPLMRFSTSTKVLEWCALGLPVVVSRTDGVTHFFSDDDVT
                     FVEPGDAQDLAAKLLELHKDPALPAERARNARETALERFDWDNEKATLIALVKRLGGL
                     A"
     gene            17267..18247
                     /locus_tag="VM841_07705"
     CDS             17267..18247
                     /locus_tag="VM841_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HVL90102.1"
                     /translation="MSLALVLGDPRDFAPAAALVAGAKDRELRAHVVSAGVAPGPDAS
                     LIEPIHVQVLLGRGSPAVREGLALMGLERELMQIRPDAIVVFGPHEVGLAAALVAAKE
                     GIPLWYAGAGRWAFAPRNLEDIGPPGVDEDLIAVLAAGMLAPTEFAATALAGARYDVK
                     RIWVTGPLEAETLERRRDEIEAENACADHNVKPAGYVVSSVADRSVLPGQPLPVVDVR
                     DLAYVERMSLAADASVVITDDDRMQIEACLLGVPCLVLAPASPLRETRAVGAAKRCPA
                     DPASIALAVAEQAARTERDWDAPPMFDADVSARIIALVAPHAARASALLD"
     gene            complement(18244..19980)
                     /locus_tag="VM841_07710"
     CDS             complement(18244..19980)
                     /locus_tag="VM841_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL90103.1"
                     /translation="MRRSLSLVLATLACLAVLPVMSAAAAAAASVAVTPVVETIVTGA
                     AHAPVATVRDASGNVLSGVAVDWQVTDGGHAADDLDGNATTPPGYIGRCQTNASGFCS
                     VTWTGTRVTVDTVRALVDLNGDGTAGSGEPAATATADWRAAGNGPSALRIDMHGCDGD
                     TAAPIDAATWNVAGPATAAGGVATICAARFTSADALAAGPVTFTITGGPGVLTDATGA
                     ADHGTSLEAQAAGGVNTVFVRSTLAGTTTVRASFGSVIADGTAAWIAGAARTIALAGP
                     AAAVSGSTVQVTATVRDRFDNPVPGVAVVFSEDGPGGFTGGAASINATTGPGGAATAA
                     VATEPGQVGSQTVAASISPADTDCELAAADPAGAPAGVCRAEAVLGWIEAPPELSLLV
                     SPDVGVWGTDFAISGVLSAGSSGIAGKNIVVSSRPAGAGPAAWTQIAIATTDENGLFV
                     ITSKPSMHTEYRAVFPGGSGYDSAVSGTDRADVRVGLHMTPSARTLRAGRPVVLAGRI
                     LPEHGNHPVHLQQLTASGWRTIATTRTDASSSYRFRVVKRSPGGLLFRTVTPSDFHHA
                     WNASLNRRVDWR"
     gene            complement(20068..22962)
                     /locus_tag="VM841_07715"
     CDS             complement(20068..22962)
                     /locus_tag="VM841_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVL90104.1"
                     /translation="MALVAAAILAPVSPATASSTLPADGYVGTDGRGWGHGRGLGQYG
                     ARGLADQGKPWTEIVAHYYRNTTIETRGAETIRVLVEDSPDVLMTSKATFSVWWSGGA
                     KIADSTSAEPFLRAAISGSQVVVQKSSSRNGPWTTLATASGPAEFRPGSEPLGLVWAS
                     GAVTYYRGTMEARLPGGSNSMLAINDLGLEHYLYGVVPREMPATWAAEAVRAQSVAAR
                     TYAVHKKDAARAASKLYDICSTTACQVYSGYATGGTTSEEPRVLEHPSANAAVEATSG
                     KVITHAGKPILAEYSSTTGGYTASGSQPYQEAVPDPTDSISPYHTWSTRIAVPSIQTA
                     WPEIGSLTGIRITKRNGFGQWGGRVSEMIISGTGGSVTVSGGTFRSKMGLRSDWFNVR
                     TYMAQLINHSETVSAPAGSQIPVAVYLKNVGNHEWRVNSSERLAIPSGQDSRFAGPDW
                     ITPAKPAAVTKNVPRPTAATVGPGEVAEFRFLLHATNVAPGTYTEMFRPLVEGLTWMN
                     DLRITITVNITTGWREDIGNMLTNGSFEHGWSAWSGYGGITSGDGPATGAVRDGSRSM
                     HLAGGGRKGIVQKLSMAGGPGRRVVIGGWARTVATSAGGGPILINLALYNSDGSVTWN
                     PVEFSRGEHEWQYVERVVQAPKVFTRAELYGVFYNQTGRVYFDGFRIFDTSLANPSFD
                     LGLASWTGSGLASGDGPSSAVVVDGHQSVRFGGGTKSLRQRVPVNGAPGRMFRIAGWT
                     KAEDANASGFTAITVIFNNHDGSQSTYTTAATAGSHEWEFVENHVAALKSFRSIDVIA
                     SVSSQSGSIYFDGLRLRENRIPNGSFDNGQAPWEPTGTWVDAVDGVASATVRDATTAL
                     KLGPANRKGMRVSLDSGGSAGVQILVSGWNKTATDNPAGSVWLSIGFRNRDGTTTWRN
                     SYFPNPAHDWVHQEMLVSAPKPYDRIDVYAMAQNPTGDVWFDGITLWALY"
     gene            complement(23106..25292)
                     /locus_tag="VM841_07720"
     CDS             complement(23106..25292)
                     /locus_tag="VM841_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90105.1"
                     /translation="MRLLRPLLLALLFLPASLGSTPVVAKPLASLDSTTAMPIALIAP
                     LAGGGGSWAAAETAPQSSVPEVASLGQASVGSQSVKAGSAAEVSPANAAAAAGGGGDE
                     PLESFVVLLKAGNSATALSRPFADAGRRHRLYEGRFGGFAATMTAAQAAALRADHRVA
                     VVERDMPVRAFATQTSAPWGLDRIDQRALPLQGLYEYTSTGKDVHAYVVDTGIKISHS
                     DFGGRADAVYDAFGGSGDDCNGHGTHVAGTVGGSTYGVAKAALLHGVRVLNCQGSGTS
                     SGVIGGLNWVANNAKKPAVVNMSLGGGASSAMDSAVASLTAAGIPVVVAAGNDSTSAC
                     NSSPAREPSAITVGSTTSSDGRSWFSNFGSCLDLFAPGSSITSAWHTSSTATASLSGT
                     SMAAPHVAGAVARYLESNTAASVTAVASAVLTAATSGTVSNAGSGSPNLLLYVDPAGS
                     GGTTPPPPANAAPVASFTHSCSGLTCSFTDTSTDDTGITGWSWSFGDGSTATTKNPSK
                     TYATEGAKTISLTVTDAGGLTSTSSTTVTVTAPATTPPSSGILLSGSISTSGSSLYAN
                     LSWSGIQGRATDVYRNGTPVMQVWSKTSAANQLPGAGTYTFRVCEAWTTTCSNEITLT
                     AGEQPSPSPSPTPPPPSTNNPPKASFSVACSAFVCSFNDTSTDDGGVVSWSWSFGDGT
                     SSTAKNPSKTYQQGFYTVTLTVADAQGLTASASKSIWLFGSGFFSG"
     gene            complement(25466..>27989)
                     /locus_tag="VM841_07725"
     CDS             complement(25466..>27989)
                     /locus_tag="VM841_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90106.1"
                     /translation="SSKGGLTAELAAVADGGDDYHAATIACFYARQTTSGGPGLNNNW
                     NLLSCDSTPTMPDASTSVFLGQGPGLEVRPDIIAEDTQNFRAFSVDVNTAALPGGITS
                     AMDVFVQVCGDGNSSYGRDGAYDGPDRNLAFPIGGLPFANNCDGVGGTIGWDYVGDVR
                     INNTRPVIRAASFPNNGYHGDALPASTSTISWQTTPDATGVSVSVCTGVDPDLGIGAG
                     RGAESTIYGGGCIIDTVPANGGPTVWTLSFGTAGPGPDVILPLGATGRAVRVEGVVCS
                     TFGCAGGPSATDTPAAHYNMFMRDNSHFARNVKLVWDYAAGPGDRTCQGGASAATSRT
                     DAWAPNVTVPPAPARGVLDVYNTQAPVLKVCVTGDSGLSIPVAGVPVAYEVVSGPGDI
                     DSDSPTYRETGWVPCTPPFSAPCSGGYRNTGAGPIFNGYSRPGNVAEPPLIDSLGRLS
                     ANVCSLGSCQRGDITNGFRTPDPLANWGVDWAAVNSLQTGTTVYRACVDLNGNLSCDA
                     GEVSATATMEWTPSGRNHNHLWLAGTAGTDGHTGTATIAAPAGGTVNLTGIAHTEGHQ
                     GLANTPVIWRIGTNSPGHFVSTEQTTDANGTADATIGSSVLDAGRTTAVLFCGDGDSD
                     GVCDQTPAQFEVNWGGVVTSSSITSFSVEPTIGVFGTDFVISGTLLDGGSNPVSSASV
                     SIQRRTLGSNDWVEIRSVISNGTGAFSMTDVDLAYNSDYRAVFPGNVSYTGATSDTDR
                     AWVRVGIAMNVSDPTVFAGQSVRISGRVLPAHPGAVVVLQVLDNGRGWKNIAAARLSS
                     SSTYAFRVTHHSSKSLIYRVAYPTQGILNAWNVSRNTRVTWA"
BASE COUNT         4336 a         9623 c         9667 g         4363 t
ORIGIN      
        1 caggagcatc gcggctacga tggctacggt tcctatgcgc acccgcacgg tgccctccct
       61 tcgcgacttg ccttggttct acttcgatac cccaatcagg atccctctcc ggcgaacgaa
      121 aggagcgtgc cgcaccgccc cacgaccccc tccggtaaat tgaaccccgc atcaaggagg
      181 aagccacgac cgcaccaggt tttcgcccgg gttttggcca tcccccgccc ccgtagccca
      241 atggatagcg cacccgcctc ctaagcggga gatgcaggtt cgattcctgc cgggggcacc
      301 cagtccacca atctcgtgcg tcgtcagccg aggcggatcg tgaccagggt ctcccgatcg
      361 gtttcggtga cctccccgat caccgatccc cacgctgcgc ccgagacgcc ggccgccgga
      421 cacaggctcc cctgctcggt tgcggagcgg agctttgccg aggtgccgct cctgcggtag
      481 ccggccgcaa ttccctcctc cattggggtg gggaacagga cgggcgagat cgccccttcg
      541 gagtcgtagg accgttcgga ctggcctccg gtccaatcga ggtcgatgcg gcagccgtcg
      601 acgtcggcat gcagccacgc cgacgagcgg tccgcggcga tgaacgaatc gccggcggcc
      661 agctcggcga agaaggacgg gccgctggtc cagcaccagc cacccggggc gctcccgtcg
      721 tggtcgcaca tcgactgttg cacgaaggcg acccgctcga tgaccccgtc gccgtcgtag
      781 tcgtcatcgc cggcgcagac gtcggtccac tcgtactcgt cccccgcggg ctcgatccag
      841 gtcgagcacg cgagcagctc gacgcgattg ctgctggtct cggtgatctc gacggggtcg
      901 gcctgggatg gcgccggcag cgcgaaggcc gcggcgacga gcgccgccaa aagacgcgct
      961 ttcatgctcg ttcctttctc gatcggtgag gagaccgctc ctggccggtc cccgagcccg
     1021 agagctccgg ccgggcgatt gagccgcccg gccgagaaac ttgccccctt tgccccgatt
     1081 gagactacaa gactcctgcc aaacatgaca agtgaaacaa tttgcctaag cgagcgttaa
     1141 gccagaggct tggttccgcg ctaccagacg gcggtcgacc agcccccgac gacgccggtg
     1201 gcgtcgcagt ccggcgactt cgtcacgcgg aaccggggtc gccgctcagg gcacgaaggg
     1261 accggagatc tcgtaggtga tgccgtcccc gtgaccgccg cgctgcgagg acacgtagag
     1321 ccgggttccc gacgggtcga acgcgggacc ggccagctcc gagtcgtggt ggccctccac
     1381 gcgaatcagc ggctcgacga ttccatcggt cgtgatgagc acgagctcca tgttcccgcc
     1441 gtcctccgcg acgagcacgt cgcccgccgg cgtgaccacg acgttgtcca cgcccgacag
     1501 cgccccgcgt tgccgtcggc cgtcgtagag aacctcgatc cgctcgtcgt cgggccggta
     1561 ggcccagacg cggtcatcgc ctttcgtggt gaagaagacc gtgcgcgtgc ggtcgtcgaa
     1621 ccagcatcct tcgccgccgc ggaagcgcgt cgatgccggc gcctgcagcc gcgtgggggt
     1681 gcggcgcgcc gacggatcct cgatctcgat ccattcgacg ctgccgtcct cgctcgcgcg
     1741 catcgcttcc aggcgtccct gctcgagcga cgggtacagc gtcggcgtga agcgatagaa
     1801 gcggccgtcg gactcgtctt ccgtcagata gagccgcttg ccgcggtcgt ccaccgccgc
     1861 ggcctcgtgc ttgaacacgc cgagcgcggg ccgggcctcc gcttcccgct cgccctgggg
     1921 atcgcactcc caaacgcggc cttgggcgaa ctcctcgcac gacagccacg atccccacgg
     1981 cgtcgggccg ccggcgcagt tgcgggtcgt tccgtcgagg atgcggtacg cgtcggcgat
     2041 ctctccttgc gcgtcgaagc ggacggcacc cacgccgccc cgcctggaaa cctcgctgtt
     2101 ggacacgtag atccatccgt cgtcgggaga ggcaaacgtc gctccgccgt cgggcgcgcg
     2161 gtgccaggtg tagcgcgatc ccgggacccg gtcgttggat gtcgcgatca cgcggctggt
     2221 gaatccgtca ggcaggcgga tgccattggc gtcgggggcc tgaagcggcc cgtagcgccg
     2281 atgcggaccg gacaccgtcg gcatcggaga catcggcgcg cgcgagcacg cggcgaaagc
     2341 gagcgccaga ccggcggact tcagaaaggc gcggcgatcc attccgccga ttcttccaca
     2401 gccgcggaat ccgcgcgccc ccaatcgcgt ccaccgaaaa aggaggaaca ccgaccgccg
     2461 ctgccgaagg agccggccat gcccgtcatc gagctggatg ccgccacgtt ccgccgccac
     2521 gtgctcgaaa gcaccacgcc ggtggtcgtc gacttctacg cggactggtg cggaccgtgc
     2581 aagcaggtcg cgccggtggt cgaggcgctc gccgcggaat gggaggacga ggtgacgttc
     2641 gccaagctcg acatcgacgc ctcgcccgag gtcgccgccg ccttcaatat ccgctcgatc
     2701 ccggcgatca tcttgttcga ggacggcgcg ccgaaggcat ggagcctggg cgcgaagccg
     2761 gggtatctgc tcgagcgcga gctcggtctc gccaagcgca agcgcgacaa ggcccgcggc
     2821 gagaagcgct catggtggcg ccggtaggca cgccttcggc ggactcgccg tgcggctccc
     2881 ggggatcgtt gcggtccgcg ccgccggggt cgctgcgcgc taggcgtcgc tgcgtcgagc
     2941 ggcctcgtcg ccgcgctgcg agcgaaccca ctcgttggtg cggtcgaaga tctgggcaac
     3001 cggccacccg ttgagcccgg cgtaggcgcc gatctgcgcc cggtgcgcga tctcgtgctc
     3061 ggccatcatc atcagcgcgc gccacgccga gatcgtgcgg tccggcgctg caatcagctc
     3121 gatccggcag gtcatcgcct cgggcggcag accctcgagc gcgtcgcgta cggccgcgaa
     3181 cgacgactcc aaggcttcgc tccactcggc ccgcgtggcg agctcggact cccacgtgtc
     3241 ccagatccag ccgcggttga ggaacgcgcc cgtgaagtac agccgtcctt cgcacaggtg
     3301 gcgcaccaga tccggcaccc cccacgccac gccgctttcg ggatcgggcg ccggcggcag
     3361 ccaccgctcg gccgcttctg ggagcagcac cacgtcgcga agcgtgcggc gatgaacgcc
     3421 ttcgaaccag cccaggaatg cgcttgcgtc cgcgaacacc ggccgcgctc ctaccggttg
     3481 aagccgggcg agcggctgct gcccggcggg tcgaccttcg aggggatcgc gacggccgag
     3541 acctcgtcga gcaggatcag aacgaacggg gtctccttgc gcacggcgtc gtcggccgac
     3601 tcgatcagca tcatgccgtc acgccgccag accatcgaac cgtggaccca gcccgcgccg
     3661 ccgcgcaacg cgaccgagac ggggtcgcct tcctcgaggt tttccaagat cgacttttcc
     3721 acggctgact cctcctacag atccaaggtg cggaagtatt cgatggtccg gcgcaagccc
     3781 tcacggacgg gaaccgccgg ctcccagccc aagacttcgc gcgcgcgggt gatgtcgggc
     3841 cggcgcacgt tcggatcgtc caccggccgc tgcatgaatg tgatctcgga cttcgactcg
     3901 gcgatctcga cgatcatgcg ggccagatcg atgacgcgat actcctcggg attgccgatg
     3961 ttgaccggct gatgctcgcc cgacttcacc agccggacga agccttcgat cagatcgctg
     4021 acgtagcaga aggatcgggt ctgcgagccg tcgccgtgca ccgtgatcgg ctcgttgcgc
     4081 aaggcctgca cgatgaagtt cgacaccgcg cggccgtcgg tcggcctcat gcgaggtccg
     4141 taggtgttgt gcgccatgat ccccgtcccg gcccagaagt tttcgcggcc ggggacgcag
     4201 aaatcgtaga cgagatccgt tgccggaacc tgttcgatcg cttgaacctg cgcccatacg
     4261 atgtcgccgg tgcgcaccgc ctgcagcacc tgttcgactc cacgatccca ttcgagcatg
     4321 tcccaaggag ccacgtgcgc gagcgtgagt cgccagaacg ggaaccgctt ctcgccggag
     4381 cgcggcttgt ccgtcgattc atatcggccg accgaaggca gcagcccgaa gcgagccagc
     4441 gccacgatca gatcgtcctt caactcatcg gatgtggtcg aaaattcatg acggatcgcc
     4501 tcggccatct tccccgagtg aacaccgtca ccttcacggt acccttccag gaaccacttc
     4561 agccgcgaga gcggaaggcc aaggatccat cctgggattc gtttgcggcc cgctccgaat
     4621 cccaggtgct cgagcacgcg aagcagcagc gttgaatgca cgaccatcga agcgctgcgc
     4681 tgtggtgtgt gcggagttcg cacgacgtgc agggcaaggt cacgctgcag gatcttcgca
     4741 gcgcggtcga gtgtttcttc ttcgcatgac agggtgatga acgccgattt cgtcgggcgt
     4801 tcctgccagc atccttcggc gacatacagg ccgaggaacc acaacagctc gtcggtgatt
     4861 tcgatgtgcg cggggagcgg cgttgatcgg ccggctgtcc tcggaaggac ccatgcttcg
     4921 tccggcacgg gaacgcctag tgcacgaagc ccggcaagcg gcacggagtt tcgctcgcgc
     4981 attccactga tcatgcccca aaccgcgctg cgccacttcg tcgcgccact ccactttccg
     5041 gtggccgcca tcgcgccgaa aagatcgaag cgtttctccc aggcaatggc accgaggccc
     5101 ggcgcgacga ccatcagatc ccacggatcg cgtccgccgg cttcccaggc ctcgatcgtg
     5161 ctgatatgcg aacgctcacg ttcgggaacc tcgatccgag cagcgaccgc aattttgtcg
     5221 ctcgtcgaaa gctgcgacac ggggcgcgcc atgggcaggc cttcgggtcc ttgaacgaac
     5281 acactgtgat ccccggtcac gcgaatcgag cggccgtagc gtgtccgcac cacgaagcac
     5341 ggacccgtcg gaggatgacc gaccaaggcc gacgcctccg acgaccggat cgcgccatcc
     5401 tggtcgaacg cgggcaccga atagccttcg agatcgatct gccctccgac gcgagcggcc
     5461 agttcgccga tcgtctcgcg acggagctgc gtgccgtcgt cgtagaggac ctgttcgtcg
     5521 gccaggatcg agttgaagat gcgcacgatg cgcgtgtcga gttcgtgctc gcggtggtag
     5581 gccatcgtca tcgcctcggc gtagcgcttc gcctcgtcgt agacgccgcg cgggccgatc
     5641 gggttgacat ggccccaata gccttcgggc tgcgggtgga tctgcgggtc gccgtagacc
     5701 tcggaggtgc tcgccaggaa gtaggtcgcc cggtgaagct tggcgatgcc cagcgtgtgg
     5761 tgcgtgccga gcgccccgac cttgagcgtc ggaatcggat gctccaggta gtcgatcggc
     5821 gaggcgggcg aggccagatg catcacgacg tccaccggct tgtccacgta cagcggcagc
     5881 gagatgttgt gctccaggaa cgagtaccgc gggtttcctg cgaacatctc gacgtgctcg
     5941 cgccggcccg tcaaaaggtt gtcgacgcag atgacctcgt cgccgtcgcc gagcagacgc
     6001 tcgatgagat gagagcccag gaagccggcg ccgccggtga ccagatagcg catcggcgcg
     6061 gagtctaccg aatccggtgt agggtcgcgc cgtggccctg atcgaagatc tgatgcccgc
     6121 ctgggacgtc cgcaagcgct acgagcgcct ggtccgcgcc ccggccggtc gaacctgggc
     6181 ggcgctgcag cgctacgaca tctcacgcaa ctcgtcggcg accaccaaag cgctgctccg
     6241 gctgcgcggc ttgcgccccg aagccggcac cggggtcggc gccttcgccg gatcccgctt
     6301 ctcggtcgtc ggcgaaacgc ccggcgcgga actggtcgtc gcgatcgcgg gcaagttctg
     6361 ggccatccgg gaatcgaatg cgctggagcc ggtcgaagac gccgcacagg tctccgccta
     6421 cgacgcgccg ggacgcgctg tggcggtctg gtcgttcgag gtcgcccccc ggaacgggcg
     6481 cagcgtgctc gccaccgaga cccgcgtgcg ctgcaccgac gaccgcgccc gccgaatctt
     6541 caaggcctac tggaccgtga tcgagccgtt cagcggcatg attcggcgcg acatgcttcg
     6601 caccgtcgcg gaaatggccg agggctcgta gaaaggcgaa gacggggcgc ctcgcagggc
     6661 accccgcctt cggtcctggt cgtgccggtt agcgccaggt caccatgtag cgctggctga
     6721 tgttccaggc atgatcccgg tcctgcgtcg ggaaggcgac gcggaacagc aggttcacgg
     6781 ccgagttcga gcggtaccag aactggtagt agttgcgcga gcccagcttc tgcttggcga
     6841 gcgtgaccca cttgccgccg aggtaggcct ggaaccagac ctcctcgccg gggtgcgccg
     6901 gcagcaccca gccgttgatg tggaaccact gaccccgctg cagcgacgtc gagttgaccc
     6961 gcgacgcgac cgcgacggcg acccagacct gctgaggtgc gctggtggaa gccaggtacg
     7021 gaccgtcgcc gttgaagatc gcgcgatact cggcgtgacg gtcgggcttg aaccagtagg
     7081 cgaaccggcc gtcggcaccg gtcgtggcgg tgacgaagga ctcgaacacg tccgtgcccc
     7141 agatgcggcg ctggatcgtg acggtcttgc cggccacgcc cgcgtcgttg cccgtcagga
     7201 agcccaccat gtaggtgccg gcgccgtaga cgagcgagtt cggcgaggac gacagcgtga
     7261 tcgcggtctc ggtgatcgac tcgacggtca tgttcgaagc ggactgagcc gctgccgagt
     7321 tgccgaaggc gtcaagggtc gagatgcgaa ccttcagcgc ctgcgtgttg agctcgggaa
     7381 cggtccacag gtagccgccg ccctccacgc cgcagccgag cgagccgctg cgcgccaggc
     7441 cggaggcgat cgagtgcgga taagtcgctc cgccgtcggt ggagtagcga agctccgcgg
     7501 cggtgaagtc gttgccggtc tcgtcgccgg tccactggat gcagacctgg tcgccgccgg
     7561 cgaaggtgcc ggtgtgcgga ccggccagcg tcaccgcgac cggcgagtag tcggccacga
     7621 cctcgaggcc ctcgctgacg gtgcagttgc tcgccgagtc gcacaggttg atcgtgagcg
     7681 tgtgcgggcc ttccgcgatg gcgctctgca gcgccgccgc gtcggcgaag gcggtcgtca
     7741 cagtgatcga ggttgcgttc tggtcgatgc cggcgatacg caccggcgtc gcgaacggct
     7801 ggccgtcgag caaaagctcg gcggtaccgc cggtggtctg gcccaccatg tggagctgac
     7861 cggcggtgat cgatgcggtc accgacaggt tgcgtgtctt gcttccgatc ttgttctggc
     7921 cgatcgacgg agcgccgtcg atctgctgcg cggccgacgc caggccggtc gccgcggacg
     7981 gcgcgaccgt gtcggcgacg acggcgaccg gggtcgcagc cacctgcgca ttgccgacgg
     8041 tgtccttcgc gccggtcagc gtgatcgagg actcgcagcc gttctgctgg ttgcagtcct
     8101 cggcggccag cgtgaccgtg ccgtcccaga tgtgcggcgc ggcgttgcgg aaaccgttgg
     8161 tgtggttggc gtcgctcacc ggcgtgatgt ctttgtcgcc gaacttcacg accggtgcga
     8221 cgttggtgtc gagcggctcg ctgaaggtcg ccgagaccga gacggtgccg gtcccgaacg
     8281 ggctggccgg ggtcgtggtg atcgagacga cgaacggctt cgctccgtcg gcgacctcgg
     8341 gcgcggccat ggtgaagccc tgcgtgtttt cgttccccgc caggtcggcg atcgagccgg
     8401 tgaacgcgag gcccacgtgg ccggtgccgt ccttggcgac agtgatcgtg accaggttgt
     8461 cggtcgcggc cggggtcggg tcgagcgcgc tcgagccgaa gcccgacgcg gccacgccgt
     8521 ccacggtgaa tgccgcggcg tcgagggagc cggcggcgac cggctcgctg aactcgatgg
     8581 tgacgcgatc ggtcgtggcg ttgttgcccg acgacagaga cgtggcgcgc accacacgcg
     8641 gcgcggctcc gtcggcggcg ttgaacgtct gtgtcttcat cgacgcgccg gcggtgtcgg
     8701 tggtgttccc ggcgctctgg ttgtaggaga cggccggcgt ggcgccggtg cccagcgagg
     8761 gcgactcggt gatcgccaag tgcagggtcg cgccgttgac ggtcatcccc gtcgccggct
     8821 ggcccgcaac gctccagcct gcctgcgtcg cggtcgaagc gactgcctcg gtgacgttaa
     8881 cgcgaacggt gtcgatcagg ccgttggcgt cgttgtcgag cgcggccgat ccgaggacgg
     8941 ccggcggaac cttgtcgagc gcctggacgc ccgccgcccc gccgaacgag ccggcgcatg
     9001 cgcgaaagct cgcgccggtc ggcacgtgcg ccttgagcgt gcacccggat ccgtcgaagg
     9061 acacgaccgg gcggacgccg ccgccccact ggacgcccgt gacgtgcacg agcagcgtgg
     9121 agtcgccggt ggcgccggtg gtaaccgagc tgaccgtcgc gatctcattg ccgttgcgaa
     9181 cggtaacctt ggaggcgagc gcgtcggtca cggtcgcgtc gtcgaggttg tgctcgaaca
     9241 tcaccgcgat cgcgtcgacg aagccgtcgc cactcgcggg ggtgaggtcc tgcgtggaca
     9301 ggcccggctc gccgttctgc gcgtagaaca ggtcggtgtt ggcgatgccg atctcgcggc
     9361 gataccgatt cacggcgccg aagtcctggt ggaccgcgtt gttcggagcg ggagcgacgc
     9421 cgtcggtgat gtggctcgcg ccggtctgat cgacgccgac gccgcatccg ccggggatgg
     9481 ccggggtctt gatgctcgag gcgttgtggg ctgcgggatc cacgagacgg acgcgcacgc
     9541 gggcgctccg ttcgccgctg gccggctcga cgatgggatc gacggtctgg cccgtcttca
     9601 ggctcgggaa gttcgtgctt ccggccggct tgttggtgtc ctgcgcgtca ccgttgcaca
     9661 cgcccagcga cgagaacttc atcgcgagct caaggttagc gatgcgcgct gccgcggtcg
     9721 cgccttcgat cctgacgggc tcgctgaagt tgaactgaag ctcgtcgggc gcctcggcga
     9781 acacgttggg cgtcccgaag tccttcgccc gggcgctcat cagaaccgga cccgcggcat
     9841 cgacgatcgt cgtgtcgttg gcgggaatct gggtgtcggg cagctcgctg ccggacgtcc
     9901 cgatggcctt ggccttctgc gaagcggccg cgtagctcac ttccggacgc agcgccgtgt
     9961 ccccgcccgt gccgaggttc ggcacgaggt tgacctgaag ctgcacggcg gagagccact
    10021 gcgaagccgg cgagccgggc tggaacccgg ccacgcggaa tccgttcgga gcggcgaccg
    10081 actggtccat caacgtgtcg aacgtcacca cgacggcgtc gagatagccg tcgccgttga
    10141 gatccttggt gtcgaccttg acgatgcccg gagtgggccc ggcggccagc gacgcttgcg
    10201 agacgagcgc cgacgctgcg acgacggcaa ataccagtac ggccttcagc ccgcgtctcg
    10261 gaacgcgcgc gaaagcaact gcacccagga ccttatcgat cacgtcgatt cacccctatg
    10321 actttctcct gctcggaatt cggatcggtt cgacggtcca aacgagggat gactgaacca
    10381 gttgcagcgg caccgacgga caatccgata ccggccgagg ggacggaccg gacgtcacgt
    10441 ccggtttcga ggagatcgag cgaatcgaac cgggcggccg accggccgaa gcggcaccga
    10501 agcggacaca cccggcatcg gtgtttttac aaagggcgaa accgacgcat cgtcgccgca
    10561 ccgctcaaaa acggcagcgg ccggacacga ggtccggccg cggatgtcgc acgcacgacc
    10621 gctagcggcc gacgccttcg taggagaagc cgagctggcg cagcgtgtgc ggatcgagca
    10681 ggttgcgggc atccacgatc gccggcgcgc gcatcgagtc gcgcagccgc tcgaagtcga
    10741 gccagcggaa ctcgttccac tcggtaagca gcagaaccgc gtcggcgccg tccgcggcct
    10801 ccatcggccc cggcgcccgc tcgatgccga gctggtcgta gaccgactcc gccacgaccg
    10861 ggtcgtagcc gcggatggtc gcgccgcgat ccatcagctc gcgcaacacg gcgatcgccg
    10921 gcgaatcccg cacgtcgtcg gtgttgggct tgaacgccag gccccaagca gcgatggtct
    10981 tgccgctcac gccaccggtg accttttcga ccttgttcgc gatgattcga tgctgctgct
    11041 cgttgacgtc gagcacgccg cgaagcagat ggaagtcgta gccgttctgc tcggcgacac
    11101 ggatcagggc cttgatgtcc ttgggaaagc acgagccccc gaagcccggc cccggcttga
    11161 ggaactcgaa gccgatccgc gcgtcgtaac ccatgcccag cgcgacctcc ttgacgtcgg
    11221 cgccgaccgc gtcgcagata ttgctgatcg cgttgatgaa gctgatcttg gtcgccagga
    11281 acgcgttgga cgcgtacttg atcatctcgg cggtttcggg gtcggtcacg atcagcggcg
    11341 cgcccagcga gcgatacagc tcggtcaaaa gacccgcggc ccgctcgctg cgcgtgccga
    11401 tcacgatccg gtcgggatgc atgaagtcga acaccgccga gccttcccgc aagaactcgg
    11461 ggttggacgc gacgtcggtc tcgacccccg gcgccagccg ctcgcgcacg atccgctcga
    11521 cccgcatcga cgagccgacc gggatcgtcg acttgttcac gatgatcttg taggtcgaga
    11581 tcagcggcgc gacgtcggtg gcgaccttct cgacgtgcga caagtcggcg ctgccgtcct
    11641 ccgccggagg cgtgggcacg cagatgaaca cgaactgggc cggccgcacc gcctcggcca
    11701 aggacgtggt gaacgagagc cgtcccgcgg ccaagttgcg ctcgacgatg tcgggcaggc
    11761 cgggctcgtg gatcgggatc tgcccgctgc gaagcttggc gaccttctcc tcgaccacgt
    11821 ccacgcagat cacgtcgtgg cccatgtcgg ccatgcacga gccggtgacc agaccgacgt
    11881 agccggtccc gacgatcgct accttcatcg ctctcccctc gcggctagac gcgctcgcgc
    11941 caatagtcga gcgtgtcctt cagggtcgtc tcgaacggga tctgcggctc ccagccggtc
    12001 accttcttga acttggtcgc gtcgcactcc agacgcggca cgtccgacgg gcgcatgcgc
    12061 tccgggtcgg tgcggatctc gacctcgacg tcgctgtagt cgagcagcgt cgagagcatg
    12121 tcgcggatct gccaggcgtt gccggacccg atgttgtaga cctcgccggg ctcgcactta
    12181 tcgagcgcga gcatgtaggc gcggatgatg tcgcgcacgt cggtgtagtc gcgataagcg
    12241 tcgaggtttc cgacgtggat caccggctcc ttcttgcctt tctcgatctc ggcgatctgc
    12301 ttggcgaagt tcgaggtcac gaacacctcg ccgcgccgcg gcccttcgtg gttgaaggcg
    12361 cgggtgcgca cgatgtacat gccgtaggac tcgtggtact ggtagcccat caggtcctgg
    12421 gcgaccttgg acaccgcgta gggcgacagc ggccggagcg ggttggactc cttgatcggc
    12481 gtctcgtcgg gatgaaccat cccgtactcc tcgctcgaac ccgcgacctg gaaccggcag
    12541 tcggtcagcc ccgcctcgcg gatcgcttcc aacaggttca cctgcgagat gatgtttgtg
    12601 ctcagcgtct cggcggggct cgcccaggag gcgtgcacgt acgactgcgc cgcgagatgg
    12661 aagacccgct cgggctggaa agcctggatc accttacgga ccccgaccgg gtcgcgcagg
    12721 tccgcgtcgt gcagcgcgac cttgcccttg acgatgccca gattctcgtc ccgggaccgc
    12781 caccggaagg tgcccgcgac ctcgtgcccc tgctcgaggc aatactcggc cagatggctt
    12841 cccacgaagc cggtcacgcc ggtgatcagc actcgcacgc gctctcaact ccttgattcg
    12901 gaagggatcg acgggccggg cccgagcccg gttctccggc gccagcgaga ctaccagagc
    12961 ggcgcccggt accctctagg ccgacgtgaa gatcgccaac atcgtgggcg cccgccccca
    13021 gttcatcaag gccgcagccg tgtgcgcggc cgcgcgcgcc gacggccgga ttcaggacta
    13081 cctgatccac accggccagc actacgacgc ggacatgagc gacatcttct tcacgcagct
    13141 gggcctgcct cgccccgacg tcgagctcgg catcggatcg ggtccgcacg gacagcagac
    13201 cgggcagatg atgatggcgc tcgagggcac cctgtcggat ctgtcgcccg acgtcacgct
    13261 cgtctacggc gacaccaaca ccacgctcgc gggcgcgctg acggcgcagc ggctcggcat
    13321 cccggtcgct cacgtcgagg ccggcctgcg ctcgttcaac cggaccatgc ccgaggagat
    13381 caaccgcatc gtcaccgacc gcgtctcggc catcctgttc tgcccgacga agacctcggt
    13441 ggccaacctc gagcgcgagg gaatcaccaa gggcgtgaac ctggtcgggg acgtgatgat
    13501 cgacgtcatg cgcggctcgg tcacgtgggc gcgcatgggg atcctcggcg agctcggcgt
    13561 ccgcaccggc gcctactatt tgatgacgct ccaccgcgcc ggcaacaccg acgacccgga
    13621 gcggctcgcg acgatcctga aggccgtagc gaagctgccg tacccggtcg tgttcccggc
    13681 ccatccacgc acccgcgcca agatgagcgc ggccggcctc gagccggaag gaaacctgcg
    13741 catgatcgag ccggtcggct atatggagtc gctcgcgctc caaatgcacg cgcgcgcgat
    13801 cctgaccgac tcgggcgggg tgcagaagga ggcatactgg ctcggcgtgc cgtgcgtgac
    13861 gttgcgcgag gacaccgaat gggtcgagac gatcgaggtg ggatggaaca tcctggctgg
    13921 cgcagacccc gagctgatcg ttgccggtgt cgaacgcttc ccgcccgagg accgcccgcc
    13981 actctacggc gacggctcga ccgcgcgcca gatcgtcgag cggctcgccg cgggcccgcc
    14041 gtgggacgcg ccgatgccca agcccgaatc gaaggcgtcc cggccgcgcc ccgccaagcc
    14101 cacccccaag cgcgcggtcg cggccgcgaa gccggctccg aagaggaagg ccgcaagcgc
    14161 ggacaacaag gccgcggcta aggcccccgc gaagaaaaag acgacgacca ccaaaaagaa
    14221 ggcatccgtg aagaagaccg ccgcgaagaa gaaggccgcc gaatgagcgc tcgcatccat
    14281 ccgaccgcga ccgtggacga cggggcgatc atcggcgagg gaaccagcgt ctggcaccag
    14341 gcgcacgtgc gggccggcgc ggtgatcggc gcgggctgca tgatcggaaa gaacgtctac
    14401 gtcggtgcgg acgttcacat cggcgaccac tgcaagatcc agaacaacgc gctgatttac
    14461 gaaggcgtct cactcggcga cggggtgttc atcggccccg gcgcgatcat cgccaacgac
    14521 cgccggcccc gggccgtcaa cccggacggc accatcaaag gtgccggtga ctggacgctg
    14581 gccgaggcgc gcatcgagca cggcgcgtcc atcggcgcgg gcgcgatcct gattccgccg
    14641 ctgatcgtgg gcgcctgggc gatggtcgcg gcgggcgcgg tcgtcgcgcg cgatgtcgag
    14701 ccacaatcgc tggtcgccgg caacccggcg cgtcacgcgg gctgggtctg cgtgtgcggc
    14761 gcccgcacgc agcccgacga caaatgcggc gactgcggcc gcatcctgga ggcatagatg
    14821 agcacgaagg tcgccgtcgt cggactgggc aagatcggct tgccgctgtc ggtccagttc
    14881 gcgctcaacg gctgcgacgt gatcgggtgc gacgtcagcg aggcgcgtgt cgccgaggtg
    14941 accaccgcga ccaacccgtt ccccgacgag gcacatctgg acgagcggct gcgtgccgcg
    15001 gtcaagcgcg gccagctgcg cgcgacgacc gacaccacgg ccgcggtccg cgacgccgac
    15061 gtcgcgatcc tgatcgtgcc gctctacgcg ggcccggacc acacgcccga cgtgtcgatc
    15121 atggagtcgg ccgccgacgc ggtcgcggac ggccttcacg acggcatgct gatcgtggtc
    15181 gagaccacga tgccggtcgg cgcgacgcga cggcttgcgc agcgcatgga acagcggtcc
    15241 ggccagacgc ccggcagcgg cttcttcatg gccttctcgc ccgagcgtgt ctactcgggg
    15301 cggatcttcg cggacctgct cgcctacccg aagctcgtcg gcggcttgga cgccgagtcg
    15361 ggcaagcgtg ccgcggcctt ctaccgctcg gtgctgcccc acgtcgaggt ccgcgagctc
    15421 gccaacgccg agaccgccga gatggcaaag ctggccgaga cgatctaccg tgacgtcaac
    15481 atcgccctcg ccaacgagct cgcccgctac gccgacggcc gcggcgtgga catcggccag
    15541 gtgattgccg cgtcgaactc gcagccgttc agccacatcc acgagccggg cgtgggcgtg
    15601 ggcggccact gcattcctca ctatccgtgg ttcgtgatcg ccgacgatcc ggaggcgacg
    15661 ctgatgccgg ccgcgcgagc gatcaacgac ggccagcccg gctgggtgct cgaccgtgtc
    15721 gcgagcgaga tcggcggcct cgagggcaag cgcgtgctcg tgctcgggct ctcgtatcgt
    15781 gcgggcgtga aagagccgac ctcctcgccc ggcatcgacc tggtgcggct gctgccggag
    15841 cgcggcgccg ccgcctacgg ccacgatccg ctcttcgacg acgacgagat cgccgcgttc
    15901 ggcgccacac ccgcgacgcc ggaggatctc ggcgagttcg atgcggtcat cgtccaggcg
    15961 gctcatccgg agtacggccg catcgactgg tccggctgca agcccggctg cgccgtcgtg
    16021 gacggccgca acacgatcga tcgcgcttcg gtcgaggccg gcggcgcgcg ctatctgggg
    16081 atcgggcgat gagacggcgt cgccgcgtgc tgatcgtcac gcactcctac tatctgcgcg
    16141 acacgcggcc gcgccgccat gccgaagcgc tcatcgacgc gggcttcgag gtggacgtgc
    16201 tctgctgccg cgacgaaggc gagccgcaga acgagcggca ccgcggcgtc aacatccggc
    16261 gcttgccttt ccgccgggag cgcggcggca aggtccgcta cctgttcgag tacgcctcgt
    16321 tcatcctcgt ggcgctgctc accgtcgctg cgatgcacct gcgtcgccgc tacaaggtcg
    16381 tctggatcct gtcgatcccc aacacgatgg tcttcaccgg gctgattccg cggctgctcg
    16441 gcacgccggt cgtgctcgac gtgcgcgacc ctatgcccga gttcttcttc tccaaatacg
    16501 ggatgtcgcc gggctccccg ctctcccggg cgctgctgac cgaagagcgc ctgtcggcgc
    16561 gcttcgcgag ccatctggtc accgtgcacc ggccgttgct cgacgcgctg ctgcgcacgg
    16621 gggtgccgcg ctcgcgcacg acggtggtga tgaacgcgcc cgacctgcgg ctgctcgacg
    16681 tgccggaccg gtcgctgcgc gatcccgacg accgcaccgt cctgtacgcc ggcacgatcg
    16741 cggcgcgcta cggcgtggac gtggcgctcg aggccgtcgc gctgctgcac agcgagattc
    16801 cgcggatccg gctgcggctc gtgggcgacg gcgatctggt cccggcgctg caagcgcgcg
    16861 cgcgcacgct cggcatcgcc gaccggatct ccttcgacgg cccggtctcg ctacccgaga
    16921 tccccgcgat cgtcgggcaa agctgggtcg gggtgcaggc gcatcgcctc gatccgttga
    16981 tgcgcttctc gacctccacc aaggtgctgg aatggtgcgc gctgggcttg ccggtcgtgg
    17041 tgtcgcgcac cgacggcgtc acgcatttct tcagcgacga cgacgtcacc ttcgtcgagc
    17101 cgggtgacgc gcaggacctg gccgcgaagc tgctcgagct gcataaagac ccggccctgc
    17161 cggccgaacg cgcgcgcaac gcgcgcgaaa ccgcgctcga gcgcttcgac tgggacaacg
    17221 agaaggccac gctgatcgcg ctggtcaagc gcctgggagg cctggcgtga gcctggcgct
    17281 ggtgctgggg gatccgcgcg acttcgcgcc ggccgcggcg ctggtggcgg gcgcgaagga
    17341 ccgcgagctt cgcgcccacg tcgtctccgc cggcgtcgcc cccggcccgg acgcgtcgct
    17401 gatcgagccg atccacgtgc aggtgctgct cggccgcggc tcgcccgcgg tgcgcgaagg
    17461 cctcgcgctg atgggcttgg aacgcgagct gatgcagatc cgacccgatg cgatcgtggt
    17521 gttcggcccc cacgaggtcg ggctggccgc cgcgctggtc gccgcgaagg aaggcatccc
    17581 gctgtggtac gcgggagccg gccgctgggc gttcgcgccg cgcaacctcg aagacatcgg
    17641 cccgcccggc gtcgacgagg atctcatcgc cgtgctcgcc gccggcatgc tcgctccgac
    17701 cgagttcgcc gccaccgcgc tcgccggcgc gcgctacgac gtcaagcgca tctgggtcac
    17761 cggcccgctc gaggccgaga cgcttgagcg ccgccgcgac gagatcgaag ccgaaaacgc
    17821 ctgcgccgac cacaacgtca aacccgccgg ctacgtcgtc tcctcggtcg cggaccgttc
    17881 cgtgctgccc ggccagccgc tcccggtcgt ggacgtgcgc gaccttgcct acgtcgagcg
    17941 aatgtcgctc gccgccgacg cgagcgtcgt gatcaccgac gacgaccgga tgcagatcga
    18001 ggcgtgcctg ctcggcgtgc cgtgcctggt gctcgcgccc gcctcgccgc tgcgcgagac
    18061 gcgcgcggtc ggcgccgcga aacgctgccc ggccgatccg gcctcgatcg cgctggcggt
    18121 cgccgagcag gcggcacgca cggagcgcga ctgggacgca cccccgatgt tcgatgccga
    18181 cgtgtccgcc cgcatcatcg cgctcgtcgc gcctcacgcg gcgcgcgcgt cggcgctgct
    18241 ggactaacgc cagtccacgc ggcggttcag gcttgcgttc caggcgtggt ggaagtcgct
    18301 cggggtcacg gtccgaaaca gcagccctcc cggcgagcgc ttgacgaccc ggaagcgata
    18361 cgaggagctc gcgtccgttc gcgtcgtggc gatggtgcgc cagccacttg cggtgagctg
    18421 ctgcagatgg accggatggt tgccgtgctc gggaaggatc cgtccggcga gcacgaccgg
    18481 ccggcccgca cgcagcgttc gcgccgacgg ggtcatgtga aggccgacgc gcacgtcggc
    18541 ccgatcggtg ccgctgaccg cgctgtcgta gcccgagccg ccgggaaaca cggcccggta
    18601 ctcggtgtgc atgctcggct tggacgtgat cacgaaaagc ccgttctcgt cggtcgtggc
    18661 gatcgcaatc tgtgtccacg cggcgggtcc cgcgccggcc gggcgtgacg agaccacgat
    18721 gttcttgccg gcgatgccgc tgcttccggc cgagagcacg ccgctgatcg cgaagtccgt
    18781 tccccacacg ccgacgtcgg gcgagacgag cagcgacagt tccggcggcg cctcgatcca
    18841 gccgagcacc gcctcggcgc ggcacacacc cgcaggcgcg ccggccggat ccgcggccgc
    18901 gagctcgcag tcggtatcgg ccggcgagat cgaggccgcc accgtctggg agcccacctg
    18961 gccgggctcg gtcgcgaccg ccgcggtcgc ggctccgccg gggccggtcg tcgcgttgat
    19021 gctcgcggcg ccaccggtga agccgcccgg cccgtcttcg gagaacacca ccgcgacgcc
    19081 cggaaccggg ttgtcgaaac ggtcccgcac cgtagccgtc acctggacgg tgctccccga
    19141 cacggcagcc gccggcccgg cgagcgcgat cgtgcgcgcg gcgccggcga tccaggcggc
    19201 ggtgccgtcg gcgatgacgg agccgaacga tgcgcgcacg gtggtggttc cggcgagcgt
    19261 ggagcgaacg aagaccgtgt tcacgccgcc ggccgcctgg gcctcgagcg aggtgccgtg
    19321 gtcggccgcg cccgtcgcat cggtgagcac gcccggtccg ccggtgatcg tgaacgtcac
    19381 cggcccggcc gcgagtgcgt cggcggaggt aaaccgggca gcgcagatcg ttgccacgcc
    19441 gcccgccgcc gtcgccgggc cggccacgtt ccaggtcgcg gcgtcgatcg gcgcggcggt
    19501 gtcgccgtcg caaccgtgca tgtcgatgcg caacgccgac ggaccgttgc cggcggcgcg
    19561 ccagtcggcc gtcgcggtcg cggcgggctc gcccgatccg gcggtgccgt cgccgttgag
    19621 gtcgacgagc gcgcgaaccg tgtccaccgt cacccgcgtg cccgtccagg tgacggagca
    19681 gaagcccgac gcgttcgtct gacagcggcc gatgtatccg ggcggagtcg tcgcgttgcc
    19741 gtcgaggtcg tcggccgcgt ggccgccgtc cgtgacctgc cagtccacgg cgacgccgct
    19801 gagcacattt ccggaggcat cccgcaccgt cgcgacgggc gcgtgcgcgg cgccggtcac
    19861 gatcgtctcc accaccggcg tcaccgcaac cgatgcggcc gcggcggcgg ctgcggacat
    19921 gacgggcagc accgcaaggc aagcgagcgt cgcgagcacg agtgaaagcg agcgtcgcat
    19981 ggatgagatc acccccgagg aaagattgaa cgatccatgc gcagcgatcc tacgacgaag
    20041 cggccgccaa acccgtgaag gtgagcgtca atacagagcc cacagcgtga tgccgtcgaa
    20101 ccagacgtcg cccgtcgggt tctgcgccat cgcgtagacg tcgatgcggt cgtagggctt
    20161 gggcgcgctc acgagcatct cctgatgcac ccagtcgtgc gccgggttcg gaaaatacga
    20221 gttgcgccat gtggtggtgc cgtcgcgatt gcggaatccg atcgacagcc acaccgaacc
    20281 ggcggggttg tcggtcgcgg tcttgttcca tcccgacacg aggatctgca caccggccga
    20341 gccgcccgaa tccaatgaga ctctcatgcc tttccggttc gcggggccca gcttgagcgc
    20401 ggtcgtcgcg tcccgcacgg tcgcgctcgc gaccccgtct accgcgtcca cccatgtgcc
    20461 ggtcggctcc cacggcgcct gtccgttgtc gaacgatccg ttcgggatcc ggttctcgcg
    20521 cagccgcagg ccgtcgaaat agatgctgcc cgactgcgac gagaccgagg cgatcacgtc
    20581 gatggaccgg aagctcttga gcgcggcaac gtggttctcg acgaactccc actcgtgcga
    20641 gccggccgtc gccgcggtcg tataggtgct ctgactgccg tcgtggttgt tgaagatcac
    20701 ggtgatggcg gtgaagccgc tcgcgttggc gtcctcggcc ttcgtccagc ccgcgatgcg
    20761 gaacatccgg ccgggcgctc cgttcaccgg cacccgctgc ctgaggctct tggtgccgcc
    20821 gccgaaccgg accgactggt gcccgtccac cacgaccgcc gagctcggcc cgtcgccgga
    20881 cgccagcccc gagccggtcc acgacgccag gccgaggtcg aaggacggat tggccagcga
    20941 ggtatcgaag atccggaagc cgtcgaagta gacccggccg gtctgattgt agaacacgcc
    21001 gtagagctcc gcgcgcgtga acaccttggg ggcctgcacg acccgctcga cgtactgcca
    21061 ctcgtgctcg ccacgcgaga actcgaccgg attccaggtc accgatccgt cgctgttgta
    21121 gagcgccagg ttgatcagga tcgggccgcc acccgcgctg gttgcgaccg tgcgcgccca
    21181 gccgccgatc accacgcgcc ggcccggccc gcccgccatg gacagcttct gcacgattcc
    21241 cttgcgtccg ccgcccgcca gatgcatcga gcggctcccg tcgcgcaccg cgccggtcgc
    21301 cgggccgtcg ccgctcgtga tgccgccgta gccgctccat gcggaccagc cgtgctcgaa
    21361 cgatccgttc gtgagcatgt tgccgatgtc ttcgcgccag ccggtcgtaa tgttgaccgt
    21421 gatcgtgatg cgaagatcgt tcatccaggt cagcccttcg acgagcggcc ggaacatctc
    21481 ggtgtaggtg ccgggcgcca cgttggtggc atgcagcaag aatcggaact cggcaacctc
    21541 gcccggcccg acggtcgcgg ccgtcgggcg cggcacgttc ttggtgacgg cggcaggctt
    21601 cgccggggtg atccagtccg gtccggcgaa gcggctgtcc tgtcccgacg gaatcgcgag
    21661 ccgctcactg ctgttgacgc gccactcgtg gttgccgacg ttcttcagat agaccgccac
    21721 cgggatctgc gagcccgccg gcgccgagac cgtctcgctg tgattgatca gctgcgccat
    21781 atacgtgcgc acgttgaacc agtcgctgcg cagccccatc ttcgagcgga acgtgccgcc
    21841 cgagacggtc acgctcccgc cggtcccgct gatgatcatc tcgctcacgc ggccgcccca
    21901 ctggccgaac ccgttgcgct tggtgatgcg gatgccggtc agcgagccga tctcgggcca
    21961 cgccgtctgg atcgaaggga ccgcgatccg cgtggaccag gtgtgatacg gcgagatgct
    22021 gtcggtcgga tccgggacgg cctcctggta gggctgcgat ccgctcgcgg tatacccgcc
    22081 ggtcgtggac gagtactcgg ccaggatcgg cttgcccgca tgcgtgatca ccttgcccga
    22141 ggtcgcctcg accgccgcgt tcgcggacgg atgctccagc acacgcggct cctccgaggt
    22201 ggtgccgccg gtcgcgtagc cgctgtagac ctggcatgcc gttgtcgagc agatgtcgta
    22261 gagcttgctc gcggcgcggg ctgcgtcctt cttgtgcacc gcgtaggtgc gcgcggcgac
    22321 ggactgcgca cgcacggcct ccgccgccca cgtcgcgggc atctcgcgcg gcaccacgcc
    22381 gtacaggtag tgctcgaggc ccagatcgtt gattgcgagc atcgagttcg agccgccggg
    22441 caggcgggcc tccatcgtgc cccggtagta ggtgaccgct ccgctcgccc acaccagacc
    22501 gagcggctcg ctcccgggcc ggaactcggc cggtccgctc gcggtcgcca gcgtcgtcca
    22561 cggcccgttg cgcgacgatg acttctgcac cacgacctgc gagccggaga tcgccgcgcg
    22621 caggaacggc tcggcggagg tcgagtccgc gatcttggct ccgccgctcc accagacgct
    22681 gaacgtcgcc ttcgacgtca tcagcacgtc gggcgagtct tcgacgagca cccggatcgt
    22741 ctcggccccg cgcgtttcga tggtggtgtt gcggtagtag tgcgcgacga tctcggtcca
    22801 gggcttgcct tggtcggcga gccccctggc tccgtactgc ccgagcccgc gtccgtgtcc
    22861 ccagccgcgc ccgtcggtgc cgacgtagcc gtcggccggc agcgtggagg aggcggtggc
    22921 cggcgagacg ggcgcgagga tcgcggccgc cacgagcgcc accgcgagca ggctccgaag
    22981 ggtggacccc gagcgcgacg caggggatcg gcgcgccggg agcaagcgtc ccggccggac
    23041 ttcggctcga ctcatgaacc gtacgatgct agcagcggaa tcgcttcgaa caagcgaagg
    23101 ccgcgctagc cggagaagaa gccgctgccg aacagccaga tgctcttgct cgccgaggcc
    23161 gtcaggcctt gcgcgtccgc gaccgtcagc gtcaccgtgt agaacccttg ctggtaggtc
    23221 ttgctcgggt tcttggccgt cgagctcgtg ccgtcgccga acgaccagga ccacgacacg
    23281 acgccaccgt catcggtgct cgtgtcgttg aacgagcaca cgaacgctga gcacgcgacc
    23341 gagaacgagg ccttgggcgg gttgttcgtc gaaggcggcg gcggagtcgg ggacggggac
    23401 ggagatggct gctctcccgc ggtcagcgtg atctcgttcg agcacgtcgt cgtccatgcc
    23461 tcgcagacgc ggaacgtata agtcccggct ccgggaagct gattggcggc cgacgtcttg
    23521 ctccacacct gcatgaccgg ggttccgttt cggtagacgt cggtcgcgcg accctggatg
    23581 ccgctccagg acaggttcgc gtacagcgac gagcccgatg tgctgatcga cccgctgagc
    23641 aggatcccgc tcgacggcgg cgtcgtcgcc ggtgccgtta cggtgaccgt ggtgctgctg
    23701 gtcgaggtca gcccgcccgc gtcggtgacg gtgagcgaga tggtcttcgc tccttccgtt
    23761 gcgtacgtct tagaggggtt cttggtcgtc gcggtgcttc cgtcgccgaa cgaccacgac
    23821 cagcccgtga ttccggtgtc gtcggtgctg gtgtcggtga aggagcaggt caagcccgag
    23881 cacgagtgcg tgaacgacgc gaccggggcg gcgttggcgg gtggcggcgg tgtggtgccg
    23941 ccgctgccgg ccggatcaac atacagaagc aggttcggcg agcccgagcc ggcgttcgag
    24001 accgtgccgc tcgtggccgc ggtgagcacg gccgaggcga ccgcggtcac cgacgccgcg
    24061 gtgttgctct cgagataccg ggcgacggct cccgcgacgt gcggcgccgc catcgaggtg
    24121 cccgacagac tcgccgtggc ggtggacgag gtgtgccacg ccgacgtgat cgagcttccc
    24181 ggcgcgaaca agtcaaggca ggatccgaag ttcgagaacc acgagcgccc gtcggagctg
    24241 gtcgtcgagc cgacggtgat cgccgagggc tcacgcgcgg gcgaggagtt gcacgcgctg
    24301 gtgctgtcgt tgccggcggc caccacgacc ggaatgccgg ccgccgtgag cgacgccacc
    24361 gcggagtcca tcgcgctcga cgcgccgcct ccgaggctca tgttgaccac ggcgggcttc
    24421 ttggcgttgt tggccaccca gttcaagcca ccgatgacgc ccgacgaggt gcccgatccc
    24481 tggcagttca ggacccgcac gccgtgaagc aaggcagcct tcgccacgcc gtacgtcgaa
    24541 ccgccgaccg ttccggccac gtgcgtgccg tggccgttgc agtcgtcgcc gcttccgccg
    24601 aaggcgtcat agacggcgtc cgcgcggccg ccgaagtcac tgtgggagat cttgatcccg
    24661 gtgtcgacga cgtaggcatg cacgtctttg cccgtcgatg tgtactcgta gagtccttgg
    24721 agcggaaggg cgcgctggtc gatccggtcc agaccccacg gcgcgctggt ctgcgtcgcg
    24781 aaggcgcgca cgggcatgtc acgctcgacc accgcgacgc gatgatcggc ccggagcgcg
    24841 gcggcctggg ctgccgtcat cgtggccgcg aagccgccga agcgaccctc gtagagacgg
    24901 tggcggcggc cggcgtcggc gaaaggacgc gacagggccg tcgccgagtt cccggccttc
    24961 agcagaacga cgaagctttc gagcggctcg tcgccgccgc caccggccgc ggcagccgcg
    25021 ttggccggcg agacttcagc cgccgagccc gccttcacgg attgggaccc gacggatgct
    25081 tgtccgagcg atgcgacttc cggaaccgat gactgcggcg cggtctccgc cgcggcccat
    25141 gaaccaccgc cgccagcgag cggagcgatc agagcaatcg gcatggcggt cgtcgagtca
    25201 agcgatgcaa gcggtttcgc gaccaccggg gtcgagccga gcgaggccgg caggaacagc
    25261 aaggcgagaa ggagcgggcg caggagtctc attctgtctc taaccttcgg agggggacgt
    25321 cgtcggctaa agcaacgatc gaagccgaat cctgcgacag tccggaccat ttccccctga
    25381 ggcgccctcc ccccgagtgc gataaccccc gaaaagcctt gtggggcgcc tttcggcgcc
    25441 ccacaagcct actgcttcgg cttccttatg cccaggtgac ccgcgtgttg cggctcacgt
    25501 tccaggcgtt gaggatgccc tgcgtcgggt aagcgacgcg gtagatcagg gacttcgacg
    25561 agtggtgcgt gacgcggaag gcgtacgtgc tcgaggagct caggcgagca gccgcaatgt
    25621 tcttccagcc acgaccgttg tcgaggacct gcaggacgac gaccgcaccc gggtgggccg
    25681 gcaggacgcg accggagatc cggacgctct gaccggcgaa caccgtcggg tccgagacgt
    25741 tcatcgcgat accgacgcgg acccacgcac ggtcggtgtc ggacgtcgcg cccgtgtagg
    25801 acacgttgcc cgggaagacc gcacggtagt cgctgttgta cgcgaggtcg acgtcggtca
    25861 tcgagaacgc gccggtgccg ttgctgatga cgctgcggat ctcgacccag tcgttcgagc
    25921 cgagcgtacg gcgctgaatc gagacactgg ccgagctgac ggggttggag ccaccgtcga
    25981 gcagcgtgcc cgagatcacg aagtcggtgc cgaagacgcc gatcgtgggc tcgaccgaga
    26041 agctcgtgat cgagctgctg gtgacaacgc caccccagtt gacctcgaac tgagccgggg
    26101 tctggtcgca gacgccgtcc gagtcgccgt caccgcagaa caggaccgcg gtggtgcgcc
    26161 cggcgtcgag cacgctcgag ccgatcgtgg cgtcggccgt gccgttggcg tcggtggtct
    26221 gctcggtcga gacgaagtgt cccggcgagt tcgttccgat gcgccagatg accggcgtgt
    26281 tggccaggcc ctggtggccc tcggtgtgcg cgataccggt caggttgacc gtgccaccgg
    26341 ccggggccgc gatggtcgcg gtgcccgtgt ggccgtcggt gccggcggtg ccggccagcc
    26401 acaggtggtt gtggttacga ccgctcggcg tccactccat ggtggcggtg gcgctgacct
    26461 cacccgcgtc gcaggaaagg ttgccgttga ggtcgacgca cgcacggtag accgtggtcc
    26521 cggtctggag cgagttcacg gctgcccagt ccacacccca gttggcgagc gggtcgggcg
    26581 tacggaagcc gttggtgatg tcaccgcgct ggcacgagcc gagcgagcag acgttcgcgc
    26641 tgaggcggcc gaggctgtcg atcagcggcg gctcggcgac gttgccggga cggctgtagc
    26701 cgttgaagat cgggcccgca ccggtgttcc ggtagccacc cgagcacgga gccgagaacg
    26761 gcggggtgca gggaacccag ccggtctcgc ggtaggtcgg gctgtcggag tcgatgtcgc
    26821 ccggacccga aacaacctcg taggcgaccg gaacgcccgc gaccgggatg ctcagacccg
    26881 agtcacccgt gacgcagacc ttcaggaccg gagcctgggt gttgtagacg tccaggacgc
    26941 cgcgggcagg agccggcggg accgtcacgt tcggggccca cgcgtcggtg cggctcgtcg
    27001 ccgccgaagc gccaccctgg caggtacggt cgccggggcc agcagcgtag tcccagacga
    27061 gcttgacgtt gcgagcaaag tgcgagttgt cccgcatgaa catgttgtag tgcgcagcgg
    27121 gggtgtcggt ggccgagggg ccgccggcgc aaccgaaggt gctgcagacc acgccctcga
    27181 cgcgcacggc acggccggtg gcacccagcg gaaggatcac gtcgggaccc gggccggcgg
    27241 tgccgaagct caaggtccag acggtcggcc caccgttcgc gggaacggtg tcgatgatgc
    27301 agcctccacc gtagatggtg gattcggcgc cacgaccggc accaatgccg aggtcggggt
    27361 cgacgcccgt gcagacggag accgagacgc ccgtggcgtc cggggtggtc tgccaggaga
    27421 tcgtgctggt cgacgccgga agcgcgtcac cgtggtagcc gttgttcggg aacgaagcgg
    27481 cgcgaatgac ggggcgggtg ttattgatac ggacgtcacc cacgtagtcc cagccgatcg
    27541 tgccaccgac gccgtcgcag ttgttcgcga acggaagacc gccgatcggg aacgcaaggt
    27601 tgcggtccgg accgtcgtag gcaccgtcac ggccgtacga gctgttgccg tcgccgcaga
    27661 cctggacgaa tacgtccatg gccgaggtga ttccgcccgg aagggccgcc gtgttcacat
    27721 cgaccgagaa cgcgcggaag ttctgcgtgt cctcggcgat gatgtcggga cggacctcca
    27781 gacccggacc ctgaccgagg aagaccgagg tcgaggcatc cggcatcgtg ggcgtcgagt
    27841 cacagctcag caggttccag ttgttgttaa gaccgggtcc gccggaagtg gtctgacgcg
    27901 cgtagaagca ggcgatcgtc gccgcgtggt agtcatcgcc accgtcggca accgccgcca
    27961 gctcggccgt caggccaccc ttcgaggac
//
LOCUS       DATLFO010000192         5188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_24582, whole genome shotgun sequence.
ACCESSION   DATLFO010000192 DATLFO010000000
VERSION     DATLFO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24582"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VM841_07730"
     CDS             <1..623
                     /locus_tag="VM841_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HVL90107.1"
                     /translation="SRAAASPSPRLLLLDEPFAALDAETRATTRQAVREHLRTHNGPA
                     LLITHDPLDAFALAQRIAVLEDGRVAQQGTPAEIRERPASRFVAELVGVNWFRGLTEP
                     GALATGGASIAAAHELPAGHDAIAVISPRAVSLHAMRPEGSPRNVFPATVVDLIHARE
                     VVRVQMDGPVPLVAEITPPAAAALALQPGTAVFAAVKATEVTVFRV"
     gene            complement(675..1757)
                     /locus_tag="VM841_07735"
     CDS             complement(675..1757)
                     /locus_tag="VM841_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HVL90108.1"
                     /translation="MTYKVTFIPGDGVGPELSEATRRVLEATGVAFEWDVQEAGADVM
                     DTYGTPLPDHVLDSIRANKLAIKGPITTPVGGGFRSVNVAIRKELDLFACLRPCKFYE
                     GVPGAFPGTDVVVVRENTEDLYAGIEFEEGSDDALKVIDFLNSIQSKTIREDSGVSIK
                     PISIFGTQRVVRFAFEHARKYGRKKVTAVHKSNIMKFSDGLFLRVAGEVANEYPDIEF
                     EERIVDNLCMQLVKRPEEYDVLVLPNLYGDIVSDLCAGLIGGLGLAPGANVGESAAVF
                     EATHGSAPKYKGQNKVNPMAMMFSGVLMLRHLGEEKSADALEQALADVIREGTSVTYD
                     LKERRDDPTAVGTSQVADAVIDKLNA"
     gene            1945..3063
                     /locus_tag="VM841_07740"
     CDS             1945..3063
                     /locus_tag="VM841_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HVL90109.1"
                     /translation="MPGAGLEDVVAAATAISDIDGKLGRLFYVGYDIHDLAPNSTFEE
                     VVYLLHHLALPTQGELDKLTDNLISERDLNPWVAALMPTFASESSPMSMLRTTVSAMS
                     AHDPDGWDNSAEANYRKAMRLISRLPNAIGSYHRLRNGLEPLRPNPKLTHAGNLLWMI
                     TGEEPDEDAARVMDICLILHADHTMNASTFAARVAASTLSDIHSAMTAAIATLKGPLH
                     GGANEAVMKMLLEIGDPSKAPDYIRKALAEKKKIMGFGHRVYKTEDPRATHLRRLSKE
                     IGERIGDMRWFEISQAIEQTVMEEKGLYPNVDFYAASVYYCLGLPIDLFTPIFAASRV
                     AGWTAHAREQYADNRLIRPESEYIGPRNQSYRPIDQRG"
     gene            3070..3534
                     /locus_tag="VM841_07745"
     CDS             3070..3534
                     /locus_tag="VM841_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL90110.1"
                     /translation="MKISEIMTKAAVVDAADDTLADAAKKMRAAQTGSLLIMDGEHLN
                     GIVTERDVLRCVAEGQDPAAMPLKDVMTTDVITISPQTSLKEAAALMADKWIRHLPVV
                     EGTKVVGIISQRDLTGVLAAALNEPEALHRIVEGSQLISERRLKRIEAGDLD"
     gene            complement(3531..4487)
                     /locus_tag="VM841_07750"
     CDS             complement(3531..4487)
                     /locus_tag="VM841_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVL90111.1"
                     /translation="MTTERTPAQQRIFDELFGREERPVWDGDIRTRIRSELEDSIRAA
                     VDAMGAAIFVGKYDLDLIHRCEGRWAAKNAKEFAWTIQMIRGRVAHTAIEIAMMDPDA
                     GAPLDLIDRAVAALSDGSEQESVATFLKGLDPIDMARLKGEANNAVVGFMSDWPKVDR
                     RWYPRTEASVRVQLFRGRVKLGAKYDMAFGPPDGMRARVVIVDFKTGKRHSSHVDDLR
                     YYALIETLKTGVPPYRVASYYLHEADFHVETVTEELLQTAIRRTADGIRRIAALQSRQ
                     PVMNPGPHCLFCPARETCEPGMHWIAAERPSAAPEYEDEDPI"
     gene            complement(4528..>5188)
                     /locus_tag="VM841_07755"
     CDS             complement(4528..>5188)
                     /locus_tag="VM841_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HVL90112.1"
                     /translation="ADLPADTTMDRLLGTIRDLNSDASVTGIIVQLPLPRGLDAVVAQ
                     EAVDPLKDVDGLHPENQGLLMLGRPRFVPATPSGVVEILHRSGIEVDGAHVVVVGRSN
                     LVGRPLAVLMGQKADGLNATVTLVHTGTRDVASFTKQADIVVVAAGRAGSLTGEMIKP
                     GASVIDVGTNRGPDGKLIGDVDFASVARVAGAITPVPGGVGPMTIAMLLKNTALAATL
                     S"
BASE COUNT          875 a         1786 c         1689 g          838 t
ORIGIN      
        1 gatccagggc ggccgcatcg ccgtcgccgc ggctgctgct gctcgacgag ccgttcgcgg
       61 cgctcgacgc ggagacgaga gcgacgacgc ggcaggccgt tcgcgagcat ctgcgcacgc
      121 acaacggccc cgcgcttctg atcacccacg atccgctcga cgcgttcgcg ctcgcgcaac
      181 gcatcgcggt gctcgaagac gggcgcgtcg cgcagcaggg cacgccggcc gagatccggg
      241 aacgtcccgc ctcgcgcttc gtcgccgagc tggtcggcgt gaactggttc cgcggtctga
      301 ccgagcccgg cgcgctcgca accggcggcg cgagcatcgc cgccgcgcac gagctgcccg
      361 ccggccacga cgccatcgcg gtgatcagcc cgcgcgcggt gtcgctgcac gcgatgcgtc
      421 cggaaggctc gccgcgtaac gtctttcccg cgaccgtggt ggatctgatc cacgcacgcg
      481 aggtggtgcg cgtgcagatg gacgggccgg tcccgctcgt cgccgagatc accccgccgg
      541 ccgcggcagc gctcgccctg caaccgggga ccgccgtttt tgccgccgtc aaggcgaccg
      601 aggtgacggt cttccgcgtc tgagcgcgaa gaggccccgg acgcgatgcc ggggcctctt
      661 gatgcttcga cgggctaagc gttgagcttg tcgatcacgg cgtcggcgac ctggctggtc
      721 ccgaccgcgg tcggatcgtc gcgacgctcc ttcaggtcgt aggtcacgct ggtgccttcg
      781 cggatcacgt cggcgagcgc ctgctcgagc gcgtcggcgg acttctcttc gcccaggtga
      841 cgcagcatca gcacgcccga gaacatcatc gccatcgggt tgaccttgtt ctggcccttg
      901 tacttggggg cgctgccgtg cgtggcttcg aacactgccg ccgactcgcc cacattcgcg
      961 cccggcgcca gcccgaggcc gccgatcaga cccgcgcaca ggtccgacac gatgtcgccg
     1021 tacagattcg gcagcaccag cacgtcgtac tcctccggcc gcttcaccag ctgcatgcac
     1081 aagttgtcga cgatgcgctc ctcgaactcg atatcggggt actcgttggc gacctcgccg
     1141 gcgacgcgca ggaacagccc gtcggagaac ttcatgatgt tggacttgtg cacggcggtg
     1201 accttcttgc ggccgtactt gcgtgcatgc tcgaacgcga agcgcaccac gcgctgcgta
     1261 ccgaagatcg agatcggctt gatcgacacg cccgagtcct cgcggatcgt cttggactgg
     1321 atcgagttga ggaaatcgat caccttcagg gcgtcgtcgg atccttcctc gaactcaatg
     1381 ccggcgtaga ggtcctcggt gttttcgcgg acgacgacga cgtcggtgcc ggggaacgcg
     1441 ccgggaacgc cttcgtagaa cttgcagggt cgcaggcagg cgaacagatc cagctctttg
     1501 cggatcgcga cgttgacgct gcggaatccg ccgccgacgg gtgtcgtgat cgggcccttg
     1561 atcgcaagct tgttggcgcg aatcgagtcc agaacatgat caggcagcgg tgtgccgtac
     1621 gtatccatca cgtcggcgcc ggcttcctgg acgtcccatt cgaacgcgac tccggtcgct
     1681 tccaggacgc ggcgcgtcgc ctcggacagc tccgggccga cgccgtcgcc cggaatgaac
     1741 gtcaccttat aggtcatcgt ggttgctcct tttcgaacgg tccccggcat tatcgccgcc
     1801 cgcgtggccg ggcgaacaat cgggcgaaca ctcgggaccg cttggcctgc cgtcactccg
     1861 atgtggcatg atggacgtgc ccggaccccc acaagaggcc gggacgcccc catcgcccag
     1921 gccaacaagt caggaaggag cgggatgcca ggagccggcc tcgaagacgt cgtcgcggcg
     1981 gcgacagcga tctcggatat cgacggcaag ctcggtcgtc tgttctacgt cggctacgac
     2041 atccacgacc tcgcaccgaa ctcgacgttc gaagaagtcg tctatctgct gcatcatttg
     2101 gctctgccca cgcaaggcga gctggacaag ctcaccgaca acttgatcag cgagcgcgac
     2161 ctgaacccat gggtcgcggc gctgatgccg acgttcgcca gcgaatcatc ccccatgtcg
     2221 atgctgcgca ccacggtttc cgcgatgagc gcgcacgacc ccgacggctg ggacaactct
     2281 gccgaagcga actaccgcaa ggcgatgcgg ctgatctcgc gcctgccgaa cgcgatcggc
     2341 tcctaccacc ggctacgcaa cggcctcgag ccgctgcgcc ccaacccgaa gctgacccac
     2401 gccggcaatc tgctgtggat gatcaccggc gaggagccgg acgaggacgc cgcgcgggtg
     2461 atggacatct gcctgatcct gcacgccgac cacaccatga acgcctcgac gttcgcggcc
     2521 cgcgtggccg cttcgacgtt gtccgacatc cactcggcga tgaccgcggc gatcgcgacg
     2581 ctcaagggac cgctgcacgg cggcgcgaac gaggccgtga tgaagatgct gctcgagatc
     2641 ggcgatccgt ccaaagcccc ggactacatc agaaaggctt tggccgagaa gaagaaaatc
     2701 atgggcttcg gccaccgcgt ctacaagacc gaggatccgc gtgccacgca cctgcgccgc
     2761 ctttcgaagg agatcggcga gcgcatcggc gacatgcgct ggttcgagat cagccaggcg
     2821 atcgagcaga cggtcatgga agagaagggc ctctacccga acgtggactt ctacgctgcc
     2881 agcgtctact actgtctcgg gctgccgatc gatctgttca cgccgatctt cgccgcgtca
     2941 cgcgtggccg gctggacggc acatgcgcgc gagcagtacg ccgacaaccg actgatccgg
     3001 cccgagtccg agtacatcgg gccgcgaaac cagagctacc gcccgatcga ccagcgaggc
     3061 taagcgcaca tgaagatcag cgagatcatg accaaggcgg cggtcgtgga cgccgcggac
     3121 gacacactcg cggacgcggc gaagaagatg cgcgccgcgc agaccggatc gctgttgatc
     3181 atggacggcg agcatctgaa cggcatcgtg accgagcgcg acgtgctgcg ctgcgtggcg
     3241 gaaggccaag accctgctgc gatgccgctg aaggacgtca tgaccaccga cgtgatcacg
     3301 atcagcccgc agacctcgct gaaggaagcg gccgcgctga tggccgacaa gtggatccgc
     3361 cacctgccgg tcgtcgaagg cacgaaggtc gtcgggatca tctcgcagcg cgacctgacc
     3421 ggcgtgctgg ccgcggcgct caacgagccc gaggcccttc accgcatcgt cgagggctcg
     3481 cagctgatca gcgagcgccg cctgaagcgt atcgaagccg gagacctgga ctagatcggg
     3541 tcttcgtctt catactccgg agcggccgat ggccgctctg ccgctatcca gtgcatgccg
     3601 ggttcgcagg tttcgcgcgc cgggcagaac aggcagtgcg gcccggggtt catcaccggc
     3661 tgacggctct gcagcgccgc gatgcgccgg atcccgtccg cggttcgccg gatcgcggtt
     3721 tgcagcagct cttcggtgac ggtctcgacg tggaagtcgg cttcgtgcag gtagtagctg
     3781 gcgacgcggt acggcggcac gccggtcttg agcgtctcga tcagcgcgta gtagcgcaga
     3841 tcgtccacgt ggctggagtg ccgctttccg gtcttgaaat cgacgatcac gacccgcgca
     3901 cgcatcccgt cgggaggccc gaacgccatg tcgtacttcg cgcccagctt cacccgcccg
     3961 cggaacagct gcacgcgcac gctggcttcg gtgcgggggt accagcgacg gtccaccttc
     4021 ggccagtcgc tcatgaatcc gacgaccgcg ttgttggcct cgcctttcag ccgggccatg
     4081 tcgatcggat ccaggccctt caggaacgtc gcgaccgact cttgctcgga tccgtcgctg
     4141 agcgccgcaa ccgcgcggtc gatcagatcg agcggcgcgc ccgcatccgg atccatcatc
     4201 gcgatctcga tcgcggtatg cgcgacgcgc ccgcggatca tctgaatcgt ccacgcgaac
     4261 tctttcgcgt tcttcgccgc ccaacggcct tcgcagcgat gaatcagatc cagatcgtat
     4321 ttgccgacga agatggccgc gcccatcgca tccacggccg cgcggatcga gtcctcgagt
     4381 tccgagcgaa tgcgagtgcg gatgtcgcca tcccaaaccg gccgctcctc gcggccgaac
     4441 agctcgtcga agatccgctg ctgcgcaggc gtccgctcgg tggtcacggg gccatgctag
     4501 ccgcaggggc cgacagcgac cagggagcta cgacagggtt gcggcgagcg cggtgttttt
     4561 cagcagcatc gcgatggtca tgggaccgac acccccggga accggcgtga tcgcccccgc
     4621 aacccgagcc accgacgcga agtccacgtc gccgatcagc ttgccgtccg ggccgcggtt
     4681 ggtgccgacg tcgatcacgc tcgcgccggg cttgatcatc tcgccggtca gcgagcccgc
     4741 gcggcccgcg gcgacgacga cgatgtcggc ctgcttcgtg aacgacgcga cgtcccgggt
     4801 gcccgtgtgg actagcgtga ccgtcgcgtt gagcccatcg gccttctgtc ccatcagcac
     4861 ggcgagcggg cggcccacca ggttcgagcg gcccaccacg accacgtgcg cgccgtccac
     4921 ttcgatgccg ctgcggtgca ggatctcgac cacgcccgac ggcgtcgcgg gaacgaaccg
     4981 cggccgtccc agcatcagca gaccttgatt ttcgggatgg agcccgtcga cgtccttgag
     5041 cggatcgacg gcttcctgcg cgaccacggc atccaaaccg cgagggagcg gcagctgcac
     5101 gatgatgccg gtcacgctcg cgtcggagtt gaggtcgcgg atcgtgccga gcaaccggtc
     5161 catggtcgtg tcggccggca gatcggcg
//
LOCUS       DATLFO010000193        31084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_106286, whole genome shotgun sequence.
ACCESSION   DATLFO010000193 DATLFO010000000
VERSION     DATLFO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 31084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106286"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VM841_07760"
     CDS             complement(<1..1069)
                     /locus_tag="VM841_07760"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVL90113.1"
                     /translation="MSRLRYGELPIWAKLLVPFVILIFVWGFFGTLVLVRSRDGEARS
                     RATAQLATAIDTARAAFADEQASLVESVRLASQLPGVREAVGSRDTAALEALLVPVAA
                     NGRHRVLRVTDARGEVLVTVDASDGDLAVSRGGSLAEPAVLAAASGIADGYGDKHLGV
                     RDREMFVAGPVLAPGAAAGGPVSGVIVVATDASRVAHELARSTGAAVTLFSAQGSALA
                     ASGRRIPFEQPGAGIQASIGGGSPFEAFYAPLLHRGRVFGTLAVGYSAEYILAGVRGT
                     AALWAILIGFGVAAATGIGIVATRAVALPVGRLVETTEELERGDLSARAAVMHGDEIG
                     RLAESFNRMAAQLEAGHAELER"
     gene            complement(1066..1482)
                     /locus_tag="VM841_07765"
     CDS             complement(1066..1482)
                     /locus_tag="VM841_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90114.1"
                     /translation="MTLLHAGPVRWIAAGAAALTVTIYSALMLGLNVPSVRAVPLAPW
                     AEGGYGADRVEEPAAAGIADPVKNHSPSSRVAGVRIPSAADSEAPAAPASPAPAPAPA
                     PPPAVPAPRPAPLLPAVPAIPSLPGIDGVLAGVGGL"
     gene            complement(1479..2156)
                     /locus_tag="VM841_07770"
     CDS             complement(1479..2156)
                     /locus_tag="VM841_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90115.1"
                     /translation="MSSAHAAFDGSSGDAAIAAARPSVSPPPSRGGLVSLLAHDLRVP
                     LGPLALATSSLAADPTIGGVAREYAQIAFVQSARVGRMMSAALAASGRLPSLRLARLA
                     MVATVRAASDAFASLGGTMTVQGVEAPVTADATVLGECLLNMAELAAGGSMRTAATVD
                     PGERVVVSFTCDDAGRCAAAFAKDTPDDADSAFALAAVAVAGAFGGLVEVTDGEIALV
                     FPPAEDA"
     gene            complement(2323..4332)
                     /gene="uvrB"
                     /locus_tag="VM841_07775"
     CDS             complement(2323..4332)
                     /gene="uvrB"
                     /locus_tag="VM841_07775"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HVL90116.1"
                     /translation="MPAFQIVSEFAPAGDQPAAIEALARGLGQGHEFQTLLGVTGSGK
                     TATTAFLVEKIQRPTLVITPNKSLGAQLASEFKELFPNNAVEYFVSYYDYYQPEAYVP
                     SSDTYIEKDSSVNDEIDRLRHSATAALLQRRDVLIVASVSCIYGLGSPEQYEQHLLRL
                     IQGVDVPMEFIKKRLVDMQYDRNDYNLARGKFRVRGDTIEIYPAYEEHILRVEMFGDT
                     VEKMTVMNPVTGEIVGDVTDTSIFAASHYAASQERMQVAINGIEAELAERLARFEREN
                     KLLEAQRLRMRTTYDIEMMREMGFCSGIENYSRHIDGRAPGEAPYTLLDYFPDDFLVV
                     IDESHVTVPQLHAMYEGDLSRKQTLVDHGFRLPSAIDNRPLRFEEFLQKLNQVVFLSA
                     TPGKYEIEVSKQVVEQVVRPTGLVDPEVIIKPTKGQIDDLIEEIRVRTERGERTLVTT
                     LTKKMAEDLTDYLLEMGLRVRYLHSNIDTIQRIEILRDLRLGEFDTLIGINLLREGLD
                     LPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVGGQVIMYADQITDSMRRALEETA
                     RRRAKQIAYNAEHGIDPQTIRKKVSDILVAAGLAEAPTTPSKAAKKKRAMRRDLEGTP
                     REELPRLIQALEDEMHEAAKELRFEYAARLRDEINELKRELREVS"
     gene            4401..5147
                     /locus_tag="VM841_07780"
     CDS             4401..5147
                     /locus_tag="VM841_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL90117.1"
                     /translation="MSIDDAHRRLQQFWDADSRTYDATSSHSVADPVEAATWRALMAR
                     VLPEAPARILDVGAGTGAMSLLAASLGHRVTALDLSTGMLERLRAKAADRGYEIEIVE
                     APADRPPAGPFDVVMERHVLWTLPDPPKALAALRAVTPGGRLVSFEGMWGQSGLAHQA
                     RRRAGKLVRQALRVPGDHHRHYEPELLAQIPLASGTTAAGLLEAIEAAGWRNARLERM
                     RDVEWARSLQERPVLRPFEGVPLFVIVADA"
     gene            complement(5185..6153)
                     /locus_tag="VM841_07785"
     CDS             complement(5185..6153)
                     /locus_tag="VM841_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HVL90118.1"
                     /translation="MSQRRIVIGALSVALAMSGCTRAGEEVRPSPRPTPTPTPTLGEP
                     VPFAPASLQFIVLDRFGPPVACDPDTYPVGRADGERHAADQWWSELNVGSTHGRAIID
                     RLGWAFPPSAAEVDRVAAYREYKKLRTVQMTLVDRAYQFEINAAPPGGSGSWDGGPAT
                     RYVGFILPDGRIESVIEHPIDVNCPVCLDGETRIRVPGGERPVRLLRQGDPVLSVGRG
                     GETIVTTVRRWVRRPAHGPLLAFELADRRRLRMAGAHPLPDGRPAGMLAPGDELGGSR
                     ILRRDAVEPSGGATYDILPDGPTGHYWANGILVASTITASPRPERH"
     gene            complement(6230..6601)
                     /locus_tag="VM841_07790"
     CDS             complement(6230..6601)
                     /locus_tag="VM841_07790"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HVL90119.1"
                     /translation="MSQQRLFTVEEANALLPTVAPMVESLRDAQREMDDRHDQVTSVA
                     AGNGGGEPGTAFLEANERAAAMLAEIQALGIVVRDPSTGLIDFPAERDGEEIFLCWRL
                     GEDDVAWWHPIDTGIAGRQPL"
     gene            complement(6598..7299)
                     /locus_tag="VM841_07795"
     CDS             complement(6598..7299)
                     /locus_tag="VM841_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HVL90120.1"
                     /translation="MPPYEAVLFDAGETLVRPEPSFGGLLHAILHDLGYVHTEDEVER
                     CARPALMTALDRATAERRPWSVSPDSSIAFWSGVYTDMLGGLGIEDRDGALATRIYRE
                     FAQPHRYGLFPDALPALRELKVRGYRLGVVSNWEDWLASLFERLGVASLFDAVVVSGS
                     EGVEKPDPAIFAIALERMGVPPERSVYVGDSLTHDVAPALSLGMGAVLIDRHRRYDGV
                     HRPTIESLGILSAVL"
     gene            complement(7362..8519)
                     /locus_tag="VM841_07800"
     CDS             complement(7362..8519)
                     /locus_tag="VM841_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HVL90121.1"
                     /translation="MHIQDVESDGISEASLQERADVVAALLRGADALTAAATELLAGG
                     GIEAVAGRPVEHMLRTDAGSTRTDASMLVQAASMLDRMPALRESFDEGLVSWGQVRSI
                     IVALRSVPATEWWRIDGLVRDHAKLLAGMDSDRLIALVDDEAARLRPDRPVRREDRAI
                     QSSFLAIQGRFDGSASLYGEADAISAATIVDALDAVADLPVHPDAGITRAQQRFGALV
                     HICERTLAGESAELRPRPRVLARIDVAALDDAGRDDALRLLWSLRGRSPRLSSVARDE
                     LLCDATVVPVLFDGPRPIAVGDQSNVFSDKTRSAIVARDGRCRFCGDAPAAWCDVHHL
                     VPGHGNTADDGCLLCRRCHRTVHRYGWTISWCEDGALEFKRRNKSFVSHPP"
     gene            8537..9271
                     /locus_tag="VM841_07805"
     CDS             8537..9271
                     /locus_tag="VM841_07805"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVL90122.1"
                     /translation="MANYCEGALANCGPDTTRSSVGLPRRAIARLLDGLILSALIMLA
                     AAAYPFWPISGSAPFIVLVIVVVVLYEWGSTTRWGATPGKQLAGIVVTHRSGSALSAR
                     TSALRMAAGTTLIFLWPLGAIALVIAIMRPRTTPLHDKITRTTVRLVPRHQPTHAADI
                     LAGLPIGVAIVSSVVMAVAAISAIPFAYAGAVWWLWPIVPGTIAQALTLGGLIWSGRR
                     RPSLAFGAFTAVTIMSLGALLRATFY"
     gene            complement(9273..9869)
                     /gene="coaE"
                     /locus_tag="VM841_07810"
     CDS             complement(9273..9869)
                     /gene="coaE"
                     /locus_tag="VM841_07810"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HVL90123.1"
                     /translation="MLLVGLTGGIASGKSTVAAMLLERGAQLIDADEIAREIVEPGLP
                     AWGKIVEHFGEQVLQADRRLDREVLAEIVFGDPAKRRLLNEITHPRIMDEMAQRIAAL
                     AGTDAIVVCDIPLLTETGRSSMFQIVVVVEAPEEVRVERLMRTRGYTREQALERIRAQ
                     ASSDDRRAVADVVIENDGDLDSLQAQVETLWSRLQSAG"
     gene            complement(10116..11216)
                     /gene="rpsA"
                     /locus_tag="VM841_07815"
                     /pseudo
     CDS             complement(10116..11216)
                     /gene="rpsA"
                     /locus_tag="VM841_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852349.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            11481..12617
                     /locus_tag="VM841_07820"
     CDS             11481..12617
                     /locus_tag="VM841_07820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HVL90124.1"
                     /translation="MAKRVLVLGGGFGGVAAAVRLRQIAPGDEVVLADRGTHFKMGFR
                     KTMEITGHGTMAEGSRPLEALERHGIRFVRGEIASIDPAARAAVVDGRTVEADALLVA
                     LGAQGKPDAVPGFLDHGLDIYGSGVAEAAETMATIESGRVVIGILGNPYPCSPAPFEL
                     ALLLDEATRARGANVTLSVFSPMPSSLPVVGPSGCEAIEGRLAGRFIDFLPNTKAERV
                     EAGRVIAEGGATIPFDVLLGIPGHVVPPVLADAGLTGPGGWVKAGPATLETGFEGVWA
                     AGDCVGMQLADGKPMPKAGVIAEGEALAAAERIAAFLRGDPSEARWNGEGGCYLETGD
                     GEAMQVQGSFLADPPDVKLLPPSRDNLEAKQRYERERLAAWFGQ"
     gene            complement(12678..14537)
                     /locus_tag="VM841_07825"
     CDS             complement(12678..14537)
                     /locus_tag="VM841_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90125.1"
                     /translation="MRIRLLLFMLGAGAIMLLPSAGIAGTCNITPASPAAGALLRGAA
                     SVSFTWSGGCGHHAPANTVSVTVNRPAVAGGGTVLLGTVTYPAATISWPTANGSFGDG
                     AGYSLTLTIGGVSAGAEESILDLTVDNTAPVIGAGLRSPAPNANGWNASAVTVDWTCT
                     DALSGVNEAATTLTDTVSDEGANQSATATCVDNAGNVATAAEPGINIDVTAPTMAVSA
                     RTPVNDAGWNNTEVEVVWSCQDALSGPVNATESDTVATEGTGQSASATCTDLAGNRTG
                     DTVEDINIDTAAPSIVFASRTPANAGGWNDSAVTVAWDCSDDLSGPEAASVETTAAGE
                     GAGQSASGICTDLAGNAAGDTVEGINIDLTAPEITFVARTPANASGWNAGPVTAEWAC
                     TDALSGAEAATVDETVTTDGAGQSASGICADLAGNTASDTVEGVNIDTVAPSIVLASR
                     TPANADGWNNTAVDIEWACDDELSGSVAGSDAATLTAEGANQSANGECADLAGNTASD
                     TVEGVSIDMTAPAAAIAGSAFYLRVLDSTMTITGVDALSGVKSMSVTFANVLGGSQTL
                     EAECVAGCGTAAATFHVDLAALDGAGIFTAQAVATDYAGNTGAPSADMMAVIV"
     gene            complement(14681..16531)
                     /locus_tag="VM841_07830"
     CDS             complement(14681..16531)
                     /locus_tag="VM841_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL90126.1"
                     /translation="MAYADVMAVRERSRRKRLVTLTSILLMPTAFLWYRIADGRPFNP
                     FALPDWHIEPIMIAPLLLILALIVLILMPMGNSKSPHVMYRPEQIEVGLDDVKGLHAV
                     VDEVVRTLNVFLGYATFRDRLGGNPRRGVLFEGPPGTGKTHLAKAMAKHAGVPYLYVS
                     GPAFQSMWYGATAKKIRSFFKALKKTARKEGGAIGFIEEIDAFATTRGGMEGSSPTHI
                     VKSGLFGRRREPTIASGTGGTVNELLIQMQSFDTLPLSWRILDKFKEWINAFLPPDAA
                     LQKRKPPYHNVLVIAATNRGDSLDPALMRPGRFDRRLYFDPPSRAGRRELVDYFLGKK
                     RHGADMTTEAKREELAAITFGYTPVMIEHLLDESLIVALRKGRDELWWEDIMEAKMTT
                     EVGVKNPVQYTTEERRQIATHEAGHAVTAYLIGEGRNLEILSIIKRRDALGLLGHSDA
                     EERFTKTKSELEALLKISLGGMVAEEIFLGESGTGPAGDLAGATGVAAEMVGSLGMAG
                     SLISFRAMGESVLDPGLVGRVLNNPEARKSVDKLLSDNKKDVTKLLTGHTRLVIALRD
                     ALLDRDELIGEEITAVLAEAGGVEAQRLARIPQPKRTRGGRSEKAASSPR"
     gene            complement(16711..19371)
                     /gene="polA"
                     /locus_tag="VM841_07835"
     CDS             complement(16711..19371)
                     /gene="polA"
                     /locus_tag="VM841_07835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVL90127.1"
                     /translation="MPAKRRAASPSASPTGPRPDVLLLLDGHSLAYRAFFALPPEMAT
                     SSGQPTNAVYGFTSMLIKLLADYGTERVAVAFDLGKPTIRLAQYAEYKAGRRETPPDF
                     SSQLGLLREVLSSLKIPVFEVPDHEADDVLATIARRAEEAGVEAVIVTADRDAMQLVR
                     PGVTVLFNRRGVSDIVMYDPPAVQERWGITPVQYLDYAALRGDPSDNLPGVPGVGEKT
                     ASKLIAQYGSVEAVFEHLDELTPKLRASLEAGKADVLRNKTLTRLVDDLPLDLDAAAI
                     RMGEWNLAQIYELFNSLEFRSLYERLEGIGRAPTDVADTFVAVTGGDAATIAAAARAA
                     GSVAVALAAGGDGLALAVHEGESTWLATRSEAIALLADPNVAITAHDAKALVRALRAG
                     DAAPGSIAMDTMLAAYLLDPASGAYDLASVLARYLKRDLPSATPPEQDGQMSLDVGDT
                     DLIEETCARAAALPRLGQHLASELERLGMMDLLRDVELPLAAVLAEMERTGIGIDVPM
                     LEGMSRELGEEIAGIETRIYELAGGPFNIGSTPQLRDVLFGRLGLKPAKKTKTGFSTD
                     AATLETLRDEHPIVPELLRYRELTKLKSTYLDALPPLIDPADGRLHATFNQAAAATGR
                     ISSENPNVQNIPVRTELGRRIRAAFVAGFPGHTLVVADYSQIELRVLAHITGDEGLRQ
                     AFANDEDIHTATAAKVWGFEPADVPKDLRSRAKMINYGLSYGMSAFGLAQRLGIAADE
                     AAEFIEAYFKGFPGVRDFMDAVVKEAYKNGYTVTALGRRRYLPELNHSNPRIRSLGER
                     QALNAPIQGTAADIIKLAMLKTESGLREAGLSARMVLTVHDELIFEVPDAQLRDTTEA
                     VRSLMEQAYPLDVPLKVDIATGRSWADAKD"
     gene            complement(19372..20034)
                     /locus_tag="VM841_07840"
     CDS             complement(19372..20034)
                     /locus_tag="VM841_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HVL90128.1"
                     /translation="MATETHGAEGYLEAVYELNEEGVTVVQARIAERLGVSRAAVSEQ
                     VRRLTAAGMVEPGAGREIVLTRHGRRVAEDAVRRHRMAERFLVDFLKMPWHLVHQEAE
                     KLQEALTGALQEHVAALLDGPGTCPHGNPIPGTGAVFRTDLVQLREFSAGDEVVLERL
                     LEDVELRTDTLKYFEEHGLMPGNKIRVVAVAPDGTMTLEVGGVSCAIGADLTDNLWVA
                     PV"
     gene            complement(20090..20566)
                     /locus_tag="VM841_07845"
     CDS             complement(20090..20566)
                     /locus_tag="VM841_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90129.1"
                     /translation="MSEITVRRATAQDLPAAQRLWQDLQAVQHRFRILPPSRDPDEVF
                     RDEFLGSDGRDDALWLIAEAGGEVAGMAYLHAEKPSRVSDEEVLELSRVAVAEGLRGR
                     GVGRALVAAAEQVARERGSRYLAARIFSRNEAAVRFWDALGFDSFLDTRMRPVDRP"
     gene            complement(20833..21564)
                     /locus_tag="VM841_07850"
     CDS             complement(20833..21564)
                     /locus_tag="VM841_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVL90130.1"
                     /translation="MTTIIRLDGLRKSFGDLEVLKGVDFRAAQGEVVCVLGPSGSGKS
                     TMLRCINRLEEPTGGHIYVEGIDIGRPGVDINKVRRHVGMVFQSFNLFPHKTALGNVM
                     LALRRVLRLSESEARGRAMSELAHVGLEDKADSYPAQLSGGQQQRVAIARALAMRPKV
                     MLFDEVTSALDPELVTEVLDVMTRLASEGMTMIVVTHEMGFARGVATRVAFIDDGLIV
                     EEGPPAQIIDRPQHERTRAFMSKIL"
     gene            complement(21561..22592)
                     /locus_tag="VM841_07855"
     CDS             complement(21561..22592)
                     /locus_tag="VM841_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVL90131.1"
                     /translation="MSAPAPRTAPGQAADPGPFGVDEHVSALARAGLIVAVAAVALAL
                     VRPLGGDASVWNRVATGAGIGAVLLGIAGRYVAGARGIRGASIGQAAIAAGALAALYA
                     YFDGAVRDGTFDPGRFWQQYFDPDVLRTLPGDFARGMVNTLKLAFLAEVLAIGVGLLV
                     ATFVLSQKFYVRYPAVGYVDLVRGLPLVVLTFLIHFGLPNIGVTFSPFISAVVILTVN
                     ASAYVAEIFRAGIQALPRGQMEAARSLGMPHAIAMTSVIVPQAARAVIPPLLSEFIAL
                     IKDTAIVIALIGFTTASRDLFGAARTAAASTFSPTPYMAAGIVYLMMTVPLSRLVGVL
                     ERRLRTKTA"
     gene            complement(22600..23523)
                     /locus_tag="VM841_07860"
     CDS             complement(22600..23523)
                     /locus_tag="VM841_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVL90132.1"
                     /translation="MRRSRIIIVAVLALALTASACGTTEQQRTPQNDEPQAIPTVELG
                     ECQTAQGTQQPTAPKGVDFKTQLKEPGKLHVGSDNAFPPFEEIKAGASEPEGFDVDLY
                     KEIAKRLGLSAVSTTTEFDGLFTQSIPTGQFDVGISAITIKEERKQTVDFTIPYFEAD
                     LSLAVNTARTPGIKSIEDLAGTTLGAQKGTTGEDCANFLVQEGKAKEVRAYNDAQAAF
                     ADLVAGRVAAIVNDRPASEGFIAKNQDLKVVQVIKTDEQYGFAISKQKPDLRVKINET
                     LEEIMKDGTYATIYEKWFGTPPPFEVPLTSG"
     gene            complement(23534..24238)
                     /locus_tag="VM841_07865"
     CDS             complement(23534..24238)
                     /locus_tag="VM841_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90133.1"
                     /translation="MSMLVLEDIRVSYGNIEAVKGISLVVEPGEIVTLIGGNGAGKTT
                     TLKTISGVRRPSKGKISFLDERIDKLAAHEIVTRGLIQSPEGRRIFPGMSVRENLEMG
                     AYHRGEPDAPDFDRVFTLFPVLKERIKQAGGTLSGGEQQMLAMGRALMGRPKLLMLDE
                     PSMGLAPMFVERIFQIITEINQQGTSILLIEQNAQMALQTAHRGYVIETGEIRLSDRA
                     DKLLGNDEVRKAYLGE"
     gene            complement(24241..25014)
                     /locus_tag="VM841_07870"
     CDS             complement(24241..25014)
                     /locus_tag="VM841_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90134.1"
                     /translation="MSLLEVDAITMRFGGLTAIDSLSFVVNEGEIRGLIGPNGAGKTT
                     FFNVVTGVYKPTEGEVRFDGSSYRGKKPHQITKAGIARTFQNIRLFPNMSAIENVMVG
                     ADVHSHTTVGAAMFGLARRSEHMSIDAGYKMLERVGIVHLASHTAKNLSYGDQRRVEI
                     ARALATRPKLLLLDEPAAGMNPAEKASLMRLIGSIRDDGVTILLIEHDMKVVMGICEQ
                     VVVIDFGQKIAEGPPEQVQRDPKVIEAYLGRGAAEGHAG"
     gene            complement(25011..26171)
                     /locus_tag="VM841_07875"
     CDS             complement(25011..26171)
                     /locus_tag="VM841_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL90135.1"
                     /translation="MRPLLALASVIAIIFAWAYGDSKVGGRVREFYDKREKWMFPVVF
                     AAFFGVFALLTTFLDVAKLELGFLVGYNALLAMGLNVVVGYAGLLDLGYVAFFAVGAY
                     TMAILSNAGPIQLPWDLGFWQILPLGIVIAMITGVILGGPTLRLRGDYLAIVTLGFGE
                     IVRIVAQNLDGVTNGARGITGVENISMFGKSLGLDPKPYYLLTLFFIGVAAILIRSLN
                     NSRVGRAWAAIREDEVAAEAMGVPTLKYKLWAFAIGAAVASVGGMVFAAKVTFIDPVS
                     FHLIISIFILSAVVLGGLGSTAGVMVGAVLINVLPEMLRGLSKLGTIGDKLLDARFGI
                     FGLVLVIMMIFRPEGIVPSRRRAAEMKGGAEETKGPTGGGLPAEPAPAGGDE"
     gene            complement(26171..27055)
                     /locus_tag="VM841_07880"
     CDS             complement(26171..27055)
                     /locus_tag="VM841_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL90136.1"
                     /translation="MVGGLTTGSIYALIALGYTLVYGVLRLINFAHSEVFAAGTFGGL
                     ITLNLIAGDGGNAFHLIVAAIPAILASAAVAVALERGAYRPLRRHGASRLSFLISAIG
                     ASLFMQSIYLIWRGRSFEQFPETIPIRPLIEIGRVQISTVDIIVFIAMIVMVVGLDFL
                     VQRTRIGKGIRAVAQDAETASMMGVNIDRVVTWTFIIGGALAGVAALLWGLRFPTRFD
                     VGFLPGIKAFTAAVLGGIGNIRGALLGGLTLGLIENVGAGCFGDAWKDVIAFTVLVLV
                     LLFRPTGILGEDVVEGKA"
     gene            complement(27158..28351)
                     /locus_tag="VM841_07885"
     CDS             complement(27158..28351)
                     /locus_tag="VM841_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVL90137.1"
                     /translation="MRIAAALVALTLVAAACGGDDGGTGQGPDSSKKTVKIGFIGDLT
                     GANSALVVPGRNAAQLAFEQANQKGDLKVTIEFVPLDNKEADPSKAPPLAQQLIGDQA
                     VVGVIGPAFSGETSAVQPLFAQAGLTHVTQSATRADLTAGGKFPTFFRSVGGDTDQAE
                     AAARIAVKGAPKCDTVAVIDDKSAYGKGYADIVDAKVKEYGGRVVTRESVAPTTDYTS
                     LVDTLRSSSPKCVVYGGYQPQSSILIKQMRQKNVAAQFISGDGTKSTKYIPEAGASNA
                     EGTLMTCPCLDANVSDEAEAKQFVTDYKAKFNTDPDIYAPEAYDVAQIFIKAIGDCGT
                     DVTRACVLDKITNLKDFKGLTKTFNWTTDKEALHEVTDKGVNIYSVKEGKIVLDGNVN
                     DIVPA"
     gene            complement(28560..29138)
                     /locus_tag="VM841_07890"
     CDS             complement(28560..29138)
                     /locus_tag="VM841_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL90138.1"
                     /translation="MAERVLIAEDESIIRMDLREMLEEEGYEVVEAGDGEAAVRLARE
                     TGPGLVILDIKMPVMDGLEAARAISEERIAPCLILTAYSQRELVEQAAEAGAMGYLVK
                     PFTKQDLLPAIEIAKGRYQQLTALADEVGDLTERLEARKVIDRAKGALMDKFAMTEAD
                     AFRFIQKASMERRLGMREVADRILDGSLGADA"
     gene            complement(29185..29466)
                     /locus_tag="VM841_07895"
     CDS             complement(29185..29466)
                     /locus_tag="VM841_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90139.1"
                     /translation="MGIGERLTALSDEIGRLRTEIAILEEQHAFVSEAAEDARIRALV
                     SETPLADREYRTAREDAERTQASLSEAKERLERLRAEQDGLLERMFEQR"
     gene            complement(29540..>31084)
                     /locus_tag="VM841_07900"
     CDS             complement(29540..>31084)
                     /locus_tag="VM841_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90140.1"
                     /translation="GLIFDLRVRLFDHVQRMPLAFFTRAQTGALISRLNNDVVGAQQA
                     VTGTLGTIVQNTIAVVSILVVMLALEWRLTLLALIVLPAFLIPSKRLGRRLQVITREQ
                     MALNAQMNTVMTERFNVSGALVAKLFGRPDEEKTTFSERAGRVRDIGIRTALFMRIFF
                     VLLGLVAAVGTAVVYYVGGRMVLSDTITIGTLAAFAAYVAQLYMPLSQLTNSRVDLMT
                     AFVSFERVFEVLDLPHAIADRPGAVVLPRPAGRIDFDHVSFRYPAGREVGLASLEEGL
                     PAQDEDAIPELVLREMSFTVEPGRTVALVGPSGAGKTTTAMLVARIYDVTDGAVRIDG
                     RDVRDVTLESLHAAIGIVSQDPHLFHDSIGNNLRFARPGASDDEIVAASKAARIHDLI
                     ASLPDGYDTVVGERGYRLSGGEKQRLAIARLLLKDPAIVILDEATSHLDSESEVLIQQ
                     ALEVALEGRTSIVIAHRLSTVVGADEIVCIDGGRIRERGSHAKLLALGGLYSELYETQ
                     FARTEA"
BASE COUNT         4739 a        10541 c        10746 g         5058 t
ORIGIN      
        1 cgcgttccag ctcggcgtgc ccggcctcga gctgcgccgc catgcggttg aacgactccg
       61 cgagacggcc gatctcgtcg ccgtgcatca ccgcggcgcg cgccgacagg tcgccgcgct
      121 cgagttcttc ggtcgtttcg acgagccggc ccacgggcag agcgaccgca cgggtcgcga
      181 cgataccgat gccggtcgcg gcggcgaccc cgaagccgat caggatggcc cacagcgcgg
      241 ccgtgccgcg caccccggca aggatgtact cggccgagta gccgacggcg agggtgccga
      301 agacgcggcc gcgatgcagc agcggtgcgt agaacgcttc gaaggggctg ccaccgccga
      361 tcgacgcctg gattcccgcg cccggttgtt cgaacgggat gcgtctgccg ctggccgcga
      421 gcgccgatcc ctgcgcggag aacagcgtca ccgccgcgcc ggtcgatcgc gcgagctcgt
      481 gagcgacacg cgatgcatcg gttgccacca cgatcactcc cgaaaccggc ccgccggctg
      541 cggcgcccgg cgcgagcacc ggcccggcga cgaacatctc acgatcccgg acaccgaggt
      601 gcttgtcgcc gtagccgtcg gcgataccgg atgccgcggc gagcacggcg ggctcggcca
      661 gggacccgcc gcgcgagacg gcgagatcgc cgtccgacgc gtccacggtc acgagcacct
      721 cgccccgcgc atcggtgacg cgcagaaccc ggtgccggcc gttggccgcg acgggaacca
      781 gaagcgcttc caaggccgcg gtgtcgcggg acccgaccgc ctcgcgcaca ccgggaagct
      841 gagacgcaag ccggaccgac tcgaccaacg aggcctgctc gtcggcaaag gcggcgcggg
      901 ccgtgtcgat cgccgtcgcg agctgggcgg tcgcgcggct gcgggcctcg ccgtcacgcg
      961 agcgcaccag caccagcgtg ccgaaaaacc cccacacgaa gatcaagatc acgaacggaa
     1021 cgagcagctt cgcccagatg ggcagttcgc cgtaccggag ccggctcata ggccgcccac
     1081 cccggcgagc acgccgtcga tgccgggcag cgacggaatc gcaggcaccg cgggcagcag
     1141 cggcgcgggc cgcggagccg gcaccgcagg agggggagcg ggagcagggg cgggagcggg
     1201 agacgccggg gccgccggtg cttcgctgtc cgcggccgac gggattcgga ccccggcaac
     1261 ccgcgacgac ggcgaatggt tcttcacggg gtccgcgatg ccggccgcgg ctggctcctc
     1321 gactcgatcg gcgccgtagc cgccctcggc ccacggggcg agcggcacgg cacggacgga
     1381 cgggacgttc aggccgagca tcaacgcgct gtagatcgtc acggtcaagg cggccgctcc
     1441 tgccgcgatc cagcgaacgg gaccggcgtg cagcagggtc atgcgtcctc cgccggaggg
     1501 aacacgagcg cgatctcgcc gtcggtcacc tcgacgagcc cgccgaacgc gccggcgacg
     1561 gcgaccgccg cgagcgcgaa cgccgagtcg gcgtcgtcgg gggtgtcctt ggcgaaggcg
     1621 gcggcgcagc gcccggcgtc atcgcacgtg aaagagacga ccacacgctc gccgggatca
     1681 accgtagcgg cggttctcat cgacccgccc gccgcgagct cggccatgtt gagcaggcac
     1741 tcgccgagca cggtggcatc ggcggtgacc ggggcttcga cgccctgcac cgtcatcgtg
     1801 ccgccgagcg aggcgaaggc gtccgatgcg gcgcgcaccg tcgcaaccat cgccaggcgc
     1861 gcgagccgga gggacggaag gcggccgctg gccgcgagcg ccgcggacat catccgtccg
     1921 acgcgcgcgc tctggacaaa ggcgatctgc gcgtactcgc gggcgacgcc gccgatcgtc
     1981 ggatcggccg ccagactcga tgtggcaagc gcgagcggcc cgagggggac gcgcaggtcg
     2041 tgggcgagca acgagacgag gccaccgcgc gatggtggtg gcgacacgga cgggcgagcg
     2101 gccgcgatgg cggcatcgcc cgacgagccg tcgaaagcgg cgtgggcaga actcatcctt
     2161 catccccctc tgcgcacggt agaggccgcg agcaagaaac ttccccaatg ttctcggagt
     2221 ccgcatcgac caccttgagt ggtggagtcg ctcccaggag gtatgctcgc tcgttcgacg
     2281 agcggcggct tttgcccgga tttgcgaggc cttttcgcgc cgtcaggaaa cttcgcgcag
     2341 ctcccgcttc agctcgttga tctcgtcccg cagccgcgct gcgtactcga agcgaagctc
     2401 tttggccgcc tcgtgcatct cgtcctccag cgcttggatc agacgcggaa gctcctcacg
     2461 cggcgtgcct tcgaggtcgc ggcgcatcgc gcgcttcttc ttggccgcct tcgacggcgt
     2521 ggtgggcgcc tcggccaggc cggccgcgac caggatgtcg ctgaccttct tgcggatggt
     2581 ctgcggatcg atgccgtgct cggcgttgta ggcgatctgc ttggcccggc gccgcgcggt
     2641 ctcttccaac gcgcggcgca tcgagtcggt gatctggtcg gcgtacatga tcacctggcc
     2701 cccgacgttt cgcgcggcac gcccgatcgt ctggatcagc gaggtctcgg accgaaggaa
     2761 gccttccttg tccgcgtcca ggatcgcgac cagcgacacc tcgggcaggt cgagcccttc
     2821 gcggagcagg ttgatgccga tgagcgtgtc gaactcgccg aggcgaaggt cgcgcaggat
     2881 ctcgatccgc tggatcgtgt cgatgttcga gtgcaggtac cgcacccgca ggcccatctc
     2941 gagcaggtag tcggtcaggt cctcggccat cttcttggtc agcgtggtga cgagcgtgcg
     3001 ctcgccccgc tcggtgcgga cgcggatctc ctcgatcagg tcgtcgatct gacccttcgt
     3061 cggcttgatg atcacctcgg gatcgaccag gccggtcggc cgcacgacct gctcgaccac
     3121 ctgcttggaa acctcgatct cgtacttgcc gggcgtggcg gacaggaaca cgacctggtt
     3181 gagcttctgc aagaactcct cgaaccggag cggccggttg tcgatcgcac tcggcagccg
     3241 gaagccgtga tcgaccagtg tctgcttgcg cgacaggtcg ccttcgtaca tcgcgtgcag
     3301 ctgcggcacg gtcacgtgcg actcgtcgat gaccaccagg aagtcgtcgg gaaaatagtc
     3361 gagcagcgtg tagggagcct cgccgggcgc gcggccgtcg atgtggcgcg agtagttctc
     3421 gatgccgctg cagaagccca tttcgcgcat catctcgatg tcgtaggtcg tgcgcatgcg
     3481 caggcgctgc gcctcgagca gtttgttctc tcgttcgaac cgggcgagcc gctcggcaag
     3541 ctcggcttcg atgccgttga tcgccacctg catgcgctcc tggctggcgg cgtagtggct
     3601 ggccgcgaag atgctggtgt cggtgacgtc gccgacgatc tcgccggtca ccgggttcat
     3661 cacggtcatc ttctcgaccg tgtccccgaa catctcgacg cgcaagatgt gctcttcgta
     3721 ggcgggatag atctcgatgg tgtcgccgcg cacccggaat ttgccgcgcg cgaggttgta
     3781 gtcgttgcgg tcgtactgca tgtcgacgag ccgcttcttg atgaactcca tcggcacgtc
     3841 cacgccttgg atcagccgca gcagatgctg ctcgtactgc tcgggcgagc ccagcccgta
     3901 gatgcacgac acgctcgcca cgatcagcac gtcgcgtcgc tgcagcagcg ccgcggttgc
     3961 gctgtggcgc agccggtcga tctcgtcgtt gaccgaggag tccttttcga tgtacgtgtc
     4021 gctggagggc acgtacgcct cgggctggta gtagtcgtag tagctgacga agtactcgac
     4081 ggcgttgttg gggaacagct ccttgaactc ggacgcgagc tgcgccccga gcgacttgtt
     4141 gggtgtgatc accagcgtcg gccgctggat cttttcgacc aggaacgcgg tcgtcgcagt
     4201 cttgcccgag ccggtcaccc cgagcaaggt ctggaactcg tggccttggc ccaggccgcg
     4261 cgcgagcgct tcgatcgccg ccggctgatc cccggcaggg gcgaactcgg acacgatctg
     4321 aaaggccggc atcgaggcgt catcgtaccg ccgggggatg acgccgggcc gggcctttcc
     4381 gatcccggta gggtgaggcc atgagcattg acgacgcgca ccgacgcctg caacagttct
     4441 gggacgccga ttcacgcacc tacgacgcga catccagcca ctcggtcgcc gacccggtcg
     4501 aggccgcgac ctggcgcgcg ctgatggcgc gcgtgctgcc ggaggccccc gctcgcatcc
     4561 tcgacgtcgg cgccggaacg ggcgcgatga gcttgctcgc cgcctcgctc gggcaccggg
     4621 tcaccgcgct cgatctgtcg accggaatgc tcgagcggct tcgcgcgaag gcggccgacc
     4681 gtggctacga gatcgagatc gtcgaggcgc ccgccgaccg gcccccggcc gggccgtttg
     4741 acgtcgtgat ggagcggcac gtgctctgga cgctgcccga tccgccgaag gcgctcgcgg
     4801 cgctgcgcgc cgtaactccc ggcggaaggc tggtgtcgtt cgaaggcatg tggggacaga
     4861 gcggcctcgc gcatcaggcg cggcggcgcg caggaaagct ggtgcgccag gcgctgcggg
     4921 ttcccggcga ccaccatcgc cactacgagc cggagctgct cgcccagatt ccgctcgcga
     4981 gcggcacgac cgccgcgggt ctgctggaag cgatcgaggc ggccggctgg cgcaacgcgc
     5041 ggctcgagcg gatgcgcgac gtcgaatggg ctcggtcgct gcaggagcgt ccggtcctgc
     5101 ggccgttcga aggcgtgccg ctgttcgtga tcgtcgccga cgcgtagcgc gcgacgctcc
     5161 agcggctaac ggccggcccc gccgtcagtg acgttctggc cggggcgatg ccgtgatggt
     5221 ggacgccaca aggattccgt tcgcccaata gtgccccgtc ggaccatccg ggaggatgtc
     5281 gtaggttgcg ccgcccgagg gctcaactgc atctcgccgc aggattcgcg agccgccgag
     5341 ctcatccccg ggggcgagca tgccggcagg gcgtccgtcc ggcagcggat gcgcgccggc
     5401 catacgcagg cgtcgcctgt cggcgagctc gaacgcgagc agcggtccgt gcgcggggcg
     5461 ccgcacccac cgacggactg tggtcacgat cgtctctccg ccgcggccga cgctcaggac
     5521 ggggtcgcct tggcggagca gacgaaccgg ccgttcgccg ccggggaccc ggatccgcgt
     5581 ctcgccgtcg aggcacaccg gacagttcac atcgattgga tgttcgatga ccgactcgat
     5641 ccggccgtcg ggcaagatga agccgacgta tcttgtcgcg ggcccgccgt cccagcttcc
     5701 cgagcctccg ggaggcgcgg cgttgatctc gaactgatac gcccgatcga ccagtgtcat
     5761 ctgcaccgtt cgcagcttct tgtactcgcg gtaggcggcg acgcgatcca cctcggccgc
     5821 cgacggcggg aacgcccacc ccagccggtc gatgatcgcc cgcccgtgcg tcgagccaac
     5881 gttgagttcg ctccaccact ggtccgccgc gtgccgttcc ccgtcggctc ggccgacggg
     5941 gtaggtgtcg ggatcgcacg cgaccggcgg accgaaccgg tccaggacga tgaactggag
     6001 cgacgccggc gcaaacggga cgggctcccc gagcgtcggg gtcggcgtgg gggtcgggcg
     6061 cggcgacggc cgcacctctt cacccgcgcg cgtgcaaccg gacatcgcca gcgcgacgct
     6121 cagcgcaccg atcacgattc gccgctggct cattctcgat tggacgccgc cggacgcttg
     6181 cgggttccct ttcgtttcgc ggggccactc ctccggagcg gagtcccgct cacagcggct
     6241 gccggccggc gataccggtg tcgatcgggt gccaccaggc gacgtcgtct tcgccgagac
     6301 gccaacatag gaagatctcc tcgccgtcgc gctcggccgg gaaatcgatc aggccggtgg
     6361 atggatcgcg taccacgatg ccgagcgcct gaatctccgc gagcatcgcc gccgcccgct
     6421 cgttcgcttc gaggaaggct gtacccggct ccccacctcc gttcccggcc gcgaccgacg
     6481 tcacctggtc gtggcggtcg tccatctcgc gctgcgcatc gcggagcgac tcgaccatcg
     6541 gcgcaaccgt tggcagaagc gcattagcct cctccaccgt gaacaggcgc tgctggctca
     6601 taggaccgcc gacaagatgc cgagggattc gatcgtaggt cgatgcacgc cgtcgtagcg
     6661 ccggtgccgg tcgatcagga ccgcgcccat cccgagcgag agtgccggcg cgacatcgtg
     6721 cgtcaggctg tcgccgacgt agacgctgcg ctccggcggc acgcccattc gttcgagcgc
     6781 gattgcgaag atcgccgggt cgggcttctc gacgccttcg gagccactca cgacgacggc
     6841 gtcgaagagg ctcgcgacgc cgagcctctc gaacaaagat gcgagccaat cctcccagtt
     6901 gctgaccacg ccgaggcgat agccgcggac cttgagctcg cgcagcgccg ggagcgcgtc
     6961 ggggaaaagg ccgtagcggt gcggctgtgc gaactcgcga tagatccggg tcgcgagcgc
     7021 gccgtcccgg tcctcgatgc cgagcccgcc gagcatgtcc gtgtagacac cgctccagaa
     7081 cgcgatcgaa gagtcgggtg agaccgacca gggccgccgt tctgccgtcg ctcggtcgag
     7141 cgccgtcata agcgcggggc gggcgcatcg ttcaacctcg tcttcggtat gtacgtagcc
     7201 gaggtcgtgc aggatcgcat gcagcaaccc gccgaaggac ggctcgggtc gcacgagcgt
     7261 ctcaccggca tcgaacaaga ctgcttcata ggggggcatg gacgagattg tatcggcccg
     7321 cgggcagcga gctttccggc gccgggtgaa cggcccgtcg tttagggcgg gtggctgacg
     7381 aaggacttgt tgcgacgctt gaactcgagc gctccgtcct cgcaccatga gatggtccag
     7441 ccgtagcggt ggacggtgcg atggcaccgg cggcacagca agcacccgtc atcggcggtg
     7501 ttgccgtggc cgggcacgag atgatggacg tcgcaccacg ccgccggcgc gtccccacag
     7561 aagcgacagc ggccgtcgcg agcgacgatg gcgctgcgcg tcttgtcgga gaacacgttc
     7621 gattgatccc cgacggcgat cggcctgggg ccgtcgaaca agacggggac gacggttgcg
     7681 tcgcagagca gctcgtcacg cgcgacgctg ctgagtcgcg gcgagcggcc gcgcagcgac
     7741 cagagaagcc gcagtgcgtc gtcgcgaccc gcgtcgtcga gcgccgcgac gtcgatgcgc
     7801 gccagcacgc gcgggcgcgg acgcagctcg gctgactcgc cggcgagtgt cctttcgcag
     7861 atgtgcacga gcgcgccgaa gcgctgctgg gcgcgggtga ttccggcatc gggatgcacc
     7921 ggaaggtcgg cgacggcatc aagcgcatcg acgatggtcg cggcagagat cgcgtcggct
     7981 tcgccgtaga ggctcgcgct gccgtcgaag cggccttgga tcgcaaggaa gctggactgg
     8041 atggcgcggt cttcgcgacg caccggccgg tcgggacgca agcgcgccgc ctcgtcgtcc
     8101 acgagggcga tcagccgatc ggagtccata ccggccagga gcttggcgtg gtcgcgcacc
     8161 aacccatcga tccgccacca ctctgttgcc ggcacactgc gaagcgcgac gataatgctg
     8221 cgaacctgac cccatgagac gagtccttcg tcgaacgact cgcgtagcgc cggcatgcgg
     8281 tccagcatgg aggcggcctg aaccagcatc gaggcgtcgg tgcgtgtcga gccggcgtcg
     8341 gtgcggagca tgtgctcgac cggccggccg gcgaccgcct cgatgccgcc gccggccagc
     8401 agctcggtcg cggccgcagt caacgcatcg gccccacgaa gcagcgcggc caccacgtcg
     8461 gctcgctcct gcagcgacgc ctccgaaatc ccgtccgatt caacgtcttg gatgtgcatc
     8521 gttcgttgta tgcgtgatgg cgaactactg cgaaggggcg ctcgcgaact gcggaccaga
     8581 cacgacgagg tcatccgtgg ggcttccgcg acgggccatt gctcggcttc tagacgggct
     8641 catcctgtct gcattgatca tgctcgcggc ggcggcctat ccgttttggc cgataagtgg
     8701 cagcgcacct ttcatcgtgc tcgtgatcgt cgtcgtcgtc ctttacgagt gggggtcgac
     8761 gactcggtgg ggcgcgacac cgggcaagca gctggccggc atcgtcgtga cgcaccgatc
     8821 cggctccgcc ctgtcagccc gtacttccgc gcttcggatg gccgcgggca ccacactgat
     8881 ctttctctgg ccgctgggcg ccatcgctct cgtgatcgcc atcatgcgtc ctcgcacgac
     8941 accattgcac gacaagatca cccgaacgac ggtccgactc gtcccccgcc accaacccac
     9001 ccacgccgcc gacatccttg cgggattgcc gatcggcgtc gcgatcgtat cgtcggtggt
     9061 gatggccgtg gcagcgattt ctgcaatccc attcgcctat gcgggagctg tgtggtggct
     9121 gtggccgatc gtgccgggga cgatcgctca ggcgttgacg ctcggcgggc tgatttggtc
     9181 aggtcgccga aggccgagcc ttgccttcgg cgccttcaca gccgtcacga tcatgagttt
     9241 gggcgcgctt ctcagagcga cgttctactg atctaccctg cgctctgcaa ccggctccac
     9301 agcgtctcga cctgggcttg gagagagtcc agatccccgt cgttttcgat cacgacgtcg
     9361 gcgacggctc gccggtcgtc ggacgaggcc tgcgctcgaa tccgctcgag cgcttgttcg
     9421 cgcgtgtagc cgcgtgtgcg catcagtcgc tcgacgcgaa cctcctcggg agcttcaacg
     9481 acgacgacga tttggaacat cgaggaccgg ccggtctcgg tcagcagcgg aatgtcgcac
     9541 accacgatcg catcggtgcc ggcgagcgcg gcgatgcgct gggccatctc gtccatgatc
     9601 cgcggatgcg taatctcatt gagcagccgc cgcttcgccg gatcgccgaa gacgatttcg
     9661 gccagcacct cgcgatccag ccgccggtcg gcctgcaaga cctgctcacc gaagtgctcg
     9721 acgatcttgc cccacgcggg caagccgggc tcgacgatct cgcgtgcgat ctcgtcggcg
     9781 tcgatcagct gcgcgccacg ctcgaggagc atggcggcaa ccgtggactt acccgatgcg
     9841 atgccgccgg tcagaccgac gagcagcacg ggtgcttagc cttcggactc ggcggcctgc
     9901 cgcttcttga tgtcggcgat gatcgcctcc agcgactcct ccgacagcgg ctgcggcttg
     9961 tccggctgcg cctccgcggc cggggcctcg acctcgggag ccatcgcggc ctcgagcacc
    10021 tcggcaccgt ccggagcagc agcggcgggc tcggcggcga gcgcttcgtc gacgagctcc
    10081 atctcgggaa ccgcgaccgg ctcgggcgcg ccttccttgg atgcctgctt gaccgacagc
    10141 gagatgcgac ggcgctccga gtcgatctcg atgatcttga ccttgaccgg gtcaccgacc
    10201 ttgacgacct gctcggccgt ctcgacgtgg cgctcggcga gctcgctgat gtgcacaaga
    10261 ccttcgatgc cgtcggcgac gcgaacgaac gcgccgaacg gaaccagctt ggtgacggcg
    10321 ccgtcgatga tctggccggg ctcgtgggtg cgcgcgaacg cggtccacgg atcttcttgc
    10381 gtcttcttca gcgacaggct gatgcgctcg cggtccaggt ccacgtccag aacctcgacg
    10441 ttgacctcct ggccgacctg cacgacctcg ctcgggtgct cgacgtgctt ccacgaaagc
    10501 tcggacacgt gaacgagccc gtccacgccg ccgaggtcca cgaacgcacc gaagttcacg
    10561 atggacgaca cgacgccctt gcgaacctca cccttggcca ggtgctcgag gaacgacttg
    10621 cgtccttcgc tctgcgcctc ttcgagatac ttgcggcgcg agagcacgac gttgttgcgg
    10681 ttgcggtcga gctcgatgat cttggcttcg atctgcttgc cgacgtacgg cgtcaggtcg
    10741 cgcacgcggc gaagctcgac cagcgacgcg ggcaggaagc cgcgcaggcc gatgtcaacg
    10801 atcagaccgc ccttgacgac ctcgatgacc gttccggtca cgacgccttc gtcgttcatg
    10861 accttttcga tctggcccca ggcacgctcg tactgcgcct tcttcttgga gaggatgagc
    10921 cgcccctctt tgtcttcctt ctgcgtgacc agcgcttcga tctcgtcgcc gaccgacacc
    10981 acttcgcgcg gatcggtgtc gtgcttgatc gacagttcgc gcgcagggat cacgccctcg
    11041 ctcttgtagc cgatgtcgag caggacctcg tccttgtcga ccttgaccac cttgccgacg
    11101 acaaggtcgc cgtcgtcgaa gttcttgatg gactgctcgt aggcggcgag cagctcttcc
    11161 ttgccaccga tgtcgttctc gatcacgtca ctggtgacgg ggtagtcctc aggcacgaag
    11221 cctcattcct ctcctcgggc cggcccggtg ccggctccgt acacagtatt tacccgtcga
    11281 gtggggtcca gggatggttt ccgccccgaa accggaaccg gatggggcgc gggaactgcc
    11341 gctgacctgc ccaaacgggc tcgcagatag tatcggcgcg gcggaagggg tgtcaacgcc
    11401 gcctctgccg ctcgcttcgg ccccgccggt agggtatgcg gcgattcctc ccgtccactt
    11461 ctctcccgga ggtcaaaggc atggcgaaac gagtgctcgt tctgggtggc ggcttcggcg
    11521 gcgtcgccgc cgcggtgcgg ctgcggcaga tcgcgcccgg cgacgaggtg gtgctcgcgg
    11581 accgcggaac gcacttcaag atgggcttcc gcaagacgat ggagatcacc ggccacggaa
    11641 ccatggccga aggcagccgg ccgctcgaag cgctggagcg ccacgggatc cgcttcgtcc
    11701 gcggcgagat cgcctcgatc gaccccgccg cccgcgccgc cgtcgtggac ggacggaccg
    11761 tcgaggccga cgcgctgctg gtcgcactgg gggcgcaggg caaaccggat gcggtgccgg
    11821 gcttcctcga ccacgggctc gacatctacg gatcgggcgt ggccgaggca gccgagacga
    11881 tggccacgat cgaatcgggc cgggtcgtca tcgggatcct gggcaacccc tatccctgct
    11941 cgcccgcgcc gttcgagctg gccttgctgc tggacgaggc gacccgggcg cggggcgcga
    12001 acgtcaccct gtcggtgttc tcgccgatgc cgtcctcgct gccggtcgtc ggcccgagcg
    12061 gctgtgaggc gatcgaaggg cggctcgccg gccgcttcat cgacttcctg cccaacacca
    12121 aggctgagcg ggtcgaggcc ggccgcgtga tcgccgaagg cggcgcgacg atcccgttcg
    12181 acgtgctgct gggcatcccc ggccacgtag tccccccggt cctggccgac gccgggctga
    12241 ccgggcccgg cggatgggtc aaggccggcc ccgcgaccct cgagaccggc ttcgagggcg
    12301 tctgggccgc gggcgactgc gtcggcatgc agctcgccga cggcaagccc atgcccaagg
    12361 ccggcgtgat cgctgaaggc gaggccctgg ccgccgcgga acggatcgcc gcgttcctgc
    12421 gcggcgatcc gagcgaggcc cggtggaacg gcgaaggcgg ctgctatctg gagaccgggg
    12481 acggcgaggc catgcaggtg cagggcagtt tcctggcgga tccgccggac gtgaagctgc
    12541 tgcccccgtc ccgcgacaac ctcgaggcca agcagcgcta cgagcgcgag cggctcgccg
    12601 cctggttcgg acaatagcgt gtgcaccgtc gaaggagggg cggccaggcc gcccctcctt
    12661 cgcgtttcgg ttccgcgtta cacgatcacg gccatcatgt ccgccgacgg cgcgccggtg
    12721 ttgccggcgt agtcggtggc gacggcctgc gccgtgaaga tcccggcccc gtccagcgcg
    12781 gccaggtcga cgtggaacgt cgctgcggcc gtgccgcatc cggcaacgca ctccgcctcg
    12841 agcgtctgcg atccgccgag cacgttggcg aaggtcacgc tcattgactt cacgccggac
    12901 agcgcgtcca cgccggtgat cgtcatcgtg ctgtcgagca cccgcaggta gaaggccgag
    12961 ccggcgatcg cggccgcggg cgcggtcatg tcgatgctca cgccctcgac ggtgtcgctc
    13021 gccgtattgc ccgcgaggtc ggcgcattcg ccgttcgcgg actggttcgc accctcggcc
    13081 gtcagggtcg cggcatccga tccggcgacc gaccccgaca gctcgtcgtc gcacgcccat
    13141 tcgatgtcga cggcggtgtt gttccagccg tcggcgttgg cgggcgtgcg cgaggcgagc
    13201 acgatcgacg gggcgacggt gtcgatgttg acaccttcga ccgtgtcgct cgcggtgttg
    13261 cccgcgaggt cggcgcagat gccggaagcg ctctggccgg cgccgtcggt cgtcacggtc
    13321 tcgtcgacgg tcgcggcttc cgcgccggac agcgcgtcgg tgcacgccca ctcggccgtg
    13381 accgggcctg cattccagcc gctggcgttc gcgggcgtgc gagcgacgaa ggtgatctcg
    13441 ggcgcggtca aatcgatgtt gatgccctcc accgtgtcgc ccgcggcgtt cccggcgagg
    13501 tcggtgcaga tgccgctggc cgactggccg gcgccttcac ccgcggcggt ggtctcgacc
    13561 gacgcggctt ccggtccgga cagatcgtcc gagcagtccc aggcgacggt cacggccgag
    13621 tcgttccagc cgccggcgtt cgccggggtg cgcgacgcga agacgatcga cggggccgcg
    13681 gtgtcgatgt tgatgtcctc gacggtgtca cccgtgcggt ttccggcgag atcggtgcag
    13741 gtcgcgctcg cagactgccc ggtcccctcg gtggccacgg tgtcggactc ggtcgcgttg
    13801 accgggcccg agagggcgtc ctggcaggac cagacgacct cgacttcggt gttgttccag
    13861 cccgcgtcgt tcacgggtgt gcgggcggac acggccatgg taggagcggt gacgtcgatg
    13921 ttgatgccgg gttcggccgc ggtcgcgacg ttgccggcgt tgtccacgca cgtcgcggtc
    13981 gcgctctggt tcgccccctc atcggagacc gtgtcggtca aggttgtggc cgcctcgttc
    14041 acgccggaca gcgcgtcggt gcacgtccaa tccaccgtca cggccgaggc gttccagccg
    14101 ttcgcgttcg gcgcggggct gcgcaggccg gcgccgatca cgggtgcggt gttgtcgacc
    14161 gtcaggtcca ggatcgactc ttccgcaccg gcgctcacac cgccgatcgt aagcgtcagc
    14221 gagtagccgg cgccgtcgcc gaacgagccg ttcgcggtcg gccagctgat cgtcgccgcc
    14281 gggtaggtca ccgtgccgag cagaaccgtc ccgccgccgg cgacggccgg gcggttcacg
    14341 gtcacggaca cggtgttggc gggcgcatgg tgcccgcagc cgcccgacca ggtgaacgac
    14401 accgaggccg cgccacgcag cagcgcgccg gccgccggcg acgccggagt gatgttgcag
    14461 gttccggcga tgccggcgct cggcaggagc atgatggccc cggccccgag catgaaaaga
    14521 agcagacgaa tacgcacgag atctccttcg agatggaccg gcctcctccg aggccataga
    14581 acgatttggg acacatccga caatgtcctg ccatcgcggg agctcgttaa tgaaagaaaa
    14641 agggaagccc tcgggcttcc ctttggtgaa cctccgatcc ttaccgcggt gacgaggctg
    14701 ccttttcgct ccgaccgcca cgcgtccgct tcggctgcgg gatgcgagcg agccgctgcg
    14761 cctccacgcc gccggcctcg gcaagaacgg cggtgatctc ctcgccgatc agctcgtcgc
    14821 ggtcgagcag cgcgtcgcgg agcgcgatca ccagacgcgt gtgccccgtc agaagcttcg
    14881 tcacgtcctt cttgttgtcg ctgaggagct tgtccacgga tttgcgggcc tcggggttgt
    14941 tgagcacgcg tccgaccagg ccggggtcga gcacgctctc ccccatcgcc cggaacgaga
    15001 tcagcgatcc cgccatgccg agcgacccga ccatctcggc cgcgacgccg gtcgcgcccg
    15061 ccaggtcgcc cgcgggtccg gtgccgctct cgccgaggaa gatctcttcg gcgaccatgc
    15121 cgcccagcga gatcttgagc agcgcctcca gctcggactt ggtcttggtg aagcgctctt
    15181 cggcgtcgga gtggccgagc agacccagcg cgtcgcgccg tttgatgatc gacaggatct
    15241 ccaggttgcg tccctcgccg atcaggtatg cggtgaccgc gtggccggct tcgtgggtcg
    15301 cgatctggcg ccgctcctcg gtcgtgtact gcaccgggtt cttcacaccg acctcggtcg
    15361 tcatcttcgc ttccatgatg tcttcccacc acagctcgtc acggcccttg cggagcgcga
    15421 cgatcagcga ttcgtcgagc agatgctcga tcatgaccgg ggtgtagccg aacgtgatcg
    15481 cggccagctc ctcgcgcttg gcttcggtgg tcatgtcggc gccgtgacgc ttcttgccga
    15541 ggaagtagtc cacgagctcc cggcggcccg cacgcgacgg cgggtcgaag tagagccggc
    15601 ggtcgaagcg gccgggacgc atcaacgccg ggtcgagcga gtcgccgcgg ttggtcgccg
    15661 cgatcacgag cacgttgtgg tacggcggct tgcgcttctg cagagccgcg tccggcggaa
    15721 ggaaggcgtt gatccattcc ttgaatttgt ccaggatccg ccacgacagc ggcagcgtgt
    15781 cgaaggactg catctggatc agaagctcgt tgacggtgcc gccggttccc gaggcgatgg
    15841 tgggctcgcg gcggcgcccg aacaggccgc tcttgacgat atgcgtcggc gaggatccct
    15901 ccattccgcc acgcgtggtc gcgaaggcat cgatctcttc gatgaagccg atcgcgccgc
    15961 cttccttgcg ggcggttttc ttcagggcct tgaagaagct gcggatcttc ttggccgtcg
    16021 cgccgtacca catggactgg aacgcgggac cggacacgta caggtaggga acgcccgcat
    16081 gcttggccat cgccttggcg aggtgcgtct tgccggtgcc cggcgggcct tcgaacagca
    16141 cgccgcggcg tgggttgccg cccaaacggt cgcggaaggt ggcgtagccc aaaaacacgt
    16201 tcagcgtgcg cacgacttcg tcgacgaccg cgtgcaggcc tttgacgtcg tcgagcccga
    16261 cttcgatctg ctcgggccgg tacatcacat gcggagactt gctgttgccc atgggcatca
    16321 ggatcagcac gatcagcgcc aggatcagaa gcagcggcgc gatcatgatc ggctcgatgt
    16381 gccagtccgg cagcgcgaac gggttaaagg ggcggccgtc ggcaatgcgg taccacagga
    16441 acgccgtcgg catcagaagg atcgacgtga gcgtgaccag acgcttgcgg cgggaacgct
    16501 cacgcaccgc catcacgtcg gcataggcca tggagagaac ttcgcggcgg ccgagcaagg
    16561 ccgcgacgct gccggcggag cccccactgc caccgggtgg attcgactgg ttcattgtgc
    16621 tcctacgcat tcgggctccc tccgaggcgt cggctctccc gacgtcctca tcctgaccgt
    16681 attcgctggg ttccgccagg ctccttctat ctagtccttt gcgtccgccc acgagcggcc
    16741 ggtcgcgata tcgaccttca gaggaacgtc cagcgggtac gcctgctcca tcaacgaacg
    16801 caccgcctcc gtggtatcgc gaagctgcgc gtcgggaacc tcgaagatga gttcatcgtg
    16861 gacggtcagg accattcggg cggacaaacc ggcttcgcgc aggcccgact cggtcttgag
    16921 catcgccagc ttgatgatgt cggcggcggt tccctggatc ggcgcgttca atgcctgccg
    16981 ttccccgagg gaacggatgc gtggattcga gtggttcagt tcgggcaggt agcggcgccg
    17041 tccgagcgcg gtgacggtgt acccgttctt gtaggcctct ttgaccacgg cgtccatgaa
    17101 gtcgcgaacg ccgggaaagc ctttgaaata cgcttcgatg aactcggcgg cctcgtcggc
    17161 cgcgatgccc agacgctgcg cgagcccgaa ggcgctcatg ccgtaggaca gcccgtagtt
    17221 gatcatcttc gcgcggctgc gcagatcttt cggcacgtcg gccggttcga agccccacac
    17281 tttcgcggca gtcgcggtat ggatgtcctc gtcgttggcg aacgcctggc gcagcccttc
    17341 gtcgccggtg atatgcgcga gcacgcgaag ctcgatctgc gagtagtcgg ccaccacgag
    17401 cgtatggccg gggaaaccgg cgacgaaggc ggcacggatg cgccggccga gctcggtgcg
    17461 cacggggatg ttctgcacgt tggggttttc gctggagatg cgtccggtcg cggcggccgc
    17521 ctggttgaag gtcgcgtgca gccgtccgtc ggcgggatcg atgagcggcg gcagcgcatc
    17581 gaggtacgtc gacttgagct tcgtgagctc gcggtagcgc agcagctccg gcacgatcgg
    17641 gtgctcgtcg cgcagggtct cgagcgtggc cgcgtcggtc gagaacccgg tcttggtctt
    17701 cttggcgggc ttgagcccga gacggccgaa cagcacgtcg cgaagctgcg gcgtcgagcc
    17761 gatgttgaac ggcccgcccg cgagctcgta gatgcgcgtc tcgatcccgg cgatctcctc
    17821 gccgagctcg cgcgacatcc cctcgagcat gggcacgtcg atcccgatcc cggtgcgctc
    17881 catctcggcg agcacggcgg caagcggaag ctcgacgtcg cggagcagat ccatcatgcc
    17941 gagccgctcg agctcggagg ccagatgctg cccgagccgc ggcagcgccg ccgcgcgcgc
    18001 gcaggtctcc tcgatcagat cggtgtcgcc gacgtccagg ctcatctgcc cgtcctgctc
    18061 gggcggcgtg gccgagggca ggtcgcgctt gaggtagcgt gcgagcaccg aggcaagatc
    18121 gtaggcgccg ctggccgggt cgagcaggta ggcggcgagc atcgtgtcca tcgcgatcga
    18181 gccgggcgcg gcgtccccgg cgcggagcgc ccgcacgagc gccttggcgt cgtgcgcggt
    18241 aatcgccaca ttcgggtcgg ccagcagcgc gatcgcctcg gagcgcgtcg ccagccaggt
    18301 ggactcgcct tcgtgcaccg cgagcgcgag cccgtcgccg ccggccgcga gtgcgaccgc
    18361 gaccgagccg gccgcgcgcg ccgccgccgc gatcgtcgcg gcgtcgccgc ccgtgacggc
    18421 gacgaacgta tcggccacgt cggtgggcgc gcggccgatg ccttcgagcc gctcgtagag
    18481 cgagcggaac tcgagcgagt tgaacagctc gtagatttga gcgaggttcc actcgcccat
    18541 ccggatcgcg gccgcgtcca ggtcgagcgg caggtcgtcc acgagccggg tcagcgtctt
    18601 gttgcgcagc acgtcggcct tgccggcctc cagcgatgcg cgcagcttcg gagtcagctc
    18661 gtcgagatgc tcgaacacgg cctcgacgga tccgtactgc gcgatcagct tcgaggcggt
    18721 tttctcgccc acgcccggca cgccgggaag gttgtcgctc ggatcgccgc gcagcgccgc
    18781 gtagtccaga tactgaaccg gcgtgattcc ccaccgctcc tgcaccgcgg gcgggtcgta
    18841 catcacgatg tcggagacgc cgcgccggtt gaagagcacg gtcacgcccg gccggaccag
    18901 ctgcatcgcg tcgcggtcgg cggtcacgat caccgcctcg acgccggcct cctcggcgcg
    18961 gcgcgcgatc gtggcgagca cgtcgtcggc ctcgtgatcg ggcacctcga acaccggaat
    19021 cttcagcgac gacagcacct cgcgcagcag gccgagctgc gagctgaagt cgggcggcgt
    19081 ctcgcgccgg ccggctttgt attcggcgta ctgggcgagg cggatggtcg gcttgccgag
    19141 gtcgaacgcg accgcgaccc gctcggtgcc gtagtccgcg agcagcttga tcagcatcga
    19201 ggtgaatccg tagaccgcgt tggtcggctg tcccgaagag gtcgccatct cggggggcag
    19261 cgcgaaaaac gcgcggtagg cgagcgagtg tccgtcgagc agaagcagca cgtcaggccg
    19321 cgggccggtc gggctggcgg agggggacgc ggcgcggcgc ttggcgggca tctagacggg
    19381 cgcgacccac aggttgtcgg tcaggtcggc gccgatcgcg catgacacgc cgccgacctc
    19441 gagcgtcatc gtgccgtcgg gcgcgaccgc gaccacgcga atcttgttgc cgggcatcag
    19501 gccgtgttcc tcgaagtatt tgagcgtgtc ggtccggagc tcgacgtcct cgagcagccg
    19561 ctcgagcacg acctcgtcac cggcggagaa ctcgcgcaac tgcaccaagt cggtgcgaaa
    19621 gaccgcgccg gtgcccggga tcgggttgcc gtgcgggcag gtgccgggcc cgtccagcag
    19681 cgccgcgacg tgctcctgca gtgcgcccgt gagcgcctcc tgcagcttct ccgcctcttg
    19741 gtggaccagg tgccagggca tcttgagaaa gtccaccagg aagcgctcgg ccatgcggtg
    19801 gcggcgcacc gcgtcctcgg cgacgcggcg gccgtgccgg gtcagcacga tctcgcggcc
    19861 cgcgccgggc tcgaccatgc ccgcggcggt gagacgtcgc acctgctcgc tgacggcggc
    19921 gcgcgagacg ccgagccgct cggcgatgcg cgcctggacg accgtaacgc cttcctcgtt
    19981 cagctcgtag acggcttcga gatagccttc ggcgccgtgc gtttcggtgg ccacgccccg
    20041 agtatattcg ccgcgccgcc gccctccggg ggcccgtccg gcggccgcgc tagggccggt
    20101 cgaccggacg catgcgggtg tcgaggaacg agtcaaaccc gagcgcgtcc cagaagcgga
    20161 ccgcggcctc gttgcgcgag aagatgcggg cggccaggta ccgggacccg cgctcgcgcg
    20221 cgacctgctc ggccgcggcg accagcgccc ggccgacgcc gcggccgcgc aggccttcgg
    20281 cgacggcgac acgcgacagc tcgagcacct cttcgtccga gacgcggctg ggcttctcgg
    20341 cgtggagata cgccatgccg gcgacctcgc cgcccgcctc ggcgatgagc caaagcgcgt
    20401 cgtcgcggcc gtcggagccg aggaactcgt cgcggaacac ctcgtcggga tcgcgggacg
    20461 gcggcaggat gcggaaccgg tgctgcaccg cctgcagatc ctgccagagc cgctgggcgg
    20521 cgggcaggtc ctgggcggtg gcccggcgga ccgtgatctc gctcacggcc gggcacaata
    20581 gccggatccc ggccacggcg aaccttgaag tggcggtcga ggcttggtgc tcgcgtcgag
    20641 ttgaccggac cgcggacgga ttgatagcgt cccgcccggc tcgtcgggaa accggcgagt
    20701 cagccccggg gtcattgcga gacgcttgcg tttggggaag gcgcgagcgt cgagccggtc
    20761 ccggggcttt ccatatcccg gccgcccgtc ccggttcttt catctccggc tctttccatc
    20821 tccgcgggcg gctcacagga tcttggacat gaacgcccgc gtccgctcgt gctgcggccg
    20881 gtcgatgatc tgtgcgggcg ggccctcctc gacgatgagc ccgtcgtcga tgaacgccac
    20941 acgcgtggcg acgccgcggg cgaaccccat ctcgtgcgtg accacgatca tcgtcatgcc
    21001 ttcgctcgca agacgcgtca tcacgtccag cacctcggtg accagctcgg ggtccagcgc
    21061 cgaggtgacc tcgtcgaaga gcatcacctt cggccgcatc gccagcgcac gcgcgatcgc
    21121 gacacgctgc tgctgcccgc ccgacagctg cgcggggtag gaatcggcct tgtcctcgag
    21181 cccgacgtgg gcgagctcgc tcatggcgcg gccgcgcgcc tccgactcgc tcaggcgcag
    21241 gaccctgcgc agcgccagca tcacgttgcc gagcgcggtc ttgtgcggga acaggttgaa
    21301 ggattgaaac accatgccga cgtgccggcg gaccttgttg atgtccaccc ccggccggcc
    21361 gatgtcgatg ccctcgacgt agatgtggcc accggtcggc tcctcgagcc ggttgatgca
    21421 gcgaagcatg gttgacttgc ccgagcccga gggcccgagc acgcagacga cctcgccctg
    21481 cgcggcgcgg aagtccacgc ctttgaggac ctcgaggtcg ccgaagctct tgcgcagccc
    21541 gtcgagccgg atgatggtcg tcatgcggtc ttggtgcgga gccggcgttc cagcacgccc
    21601 acgagccggg acagcggaac ggtcatcatc aggtacacga tccccgccgc catgtacggg
    21661 gtgggcgaga aggtcgaggc ggccgcggtg cgggccgccc cgaacaggtc ccggctggcg
    21721 gtggtgaagc cgatcagcgc gatcacgatc gcggtgtcct tgatcagcgc gatgaactcg
    21781 ctcagcagcg gcgggatgac cgcgcgggcg gcttgcggca cgatcacgct cgtcatcgcg
    21841 atggcgtgcg gcatgcccag gctgcgggcc gcctccatct ggccgcgtgg cagcgcctgg
    21901 atcccggcgc ggaagatctc ggcgacgtag gccgaggcgt tgacggtcag gatcaccacc
    21961 gccgatatga acgggctgaa ggtcacgccg atgttgggca ggccgaagtg gatcaggaac
    22021 gtgagcacga ccagcggcag gccgcgcacc aggtccacgt agccgaccgc cggatagcgc
    22081 acgtagaact tctgggacag cacgaacgtc gcgacgagca gccccacccc gatggcgagc
    22141 acctcggcca ggaaggcgag cttgagcgtg ttgaccatcc cgcgcgcgaa gtcgcccgga
    22201 agcgtgcgca gcacgtcggg gtcgaagtac tgctgccaga agcggcccgg atcgaacgtg
    22261 ccgtcgcgca ccgcgccgtc gaaataggcg tagagcgcgg caagcgctcc cgccgcgatc
    22321 gccgcctggc cgatcgaggc gccgcggatc ccgcgcgccc cggcgacgta gcggccggcg
    22381 atgccgagca ggaccgcgcc gatccccgcg ccggtcgcga cgcggttcca caccgaggcg
    22441 tcgccgccga gcggccgcac gagcgcgagc gcgacggcgg cgacggcgac gatcagaccg
    22501 gcgcgggcga gcgcgctgac gtgctcgtcg acgccgaacg gacccggatc cgccgcctgc
    22561 ccgggcgccg tgcggggggc gggagcgctc atggcgcggc tatccgctcg tgagcggaac
    22621 ttcgaagggc ggcggcgtcc cgaaccactt ctcgtagatc gtggcgtagg tgccgtcctt
    22681 catgatctcc tcgagcgtct cgttgatctt gactcgcaaa tcgggcttct gcttgctgat
    22741 cgcgaatccg tactgctcgt cggtcttgat cacctgcacg accttgagat cctggttctt
    22801 ggcgatgaag ccctccgatg ccggccggtc gttgacgatc gcggcgacgc ggccggcgac
    22861 gaggtcggcg aacgcggcct gcgcgtcgtt gtaggcgcgg acctccttgg ccttgccctc
    22921 ctggaccagg aagtttgcgc agtcctcgcc ggtggtgccc ttttgcgctc cgagcgtcgt
    22981 gccggccagg tcctcgatcg acttgatgcc gggcgtacgc gccgtgttga ccgcgagcga
    23041 caggtcggcc tcgaagtacg ggatcgtgaa gtcgacggtc tgcttgcgct cttccttgat
    23101 cgtgatcgcc gagatgccga cgtcgaactg gccggtcggg atcgactgcg tgaacaggcc
    23161 gtcgaactcg gtggtcgtgg acaccgcgga caggccgagg cgcttggcga tctccttgta
    23221 caggtccacg tcgaagcctt ccggctcgga ggccccggcc ttgatctcct cgaacggcgg
    23281 gaacgcgtta tccgagccga cgtgaagctt gccgggctcc ttgagctggg tcttgaagtc
    23341 cacgcccttc ggggcggtgg gctgctgcgt cccttgtgcc gtctggcatt cgcccagctc
    23401 gacggtcggg atcgcctgcg gctcgtcgtt ctgcggcgtc cgctgctgct cggtggtgcc
    23461 gcacgcgctc gcggtcaacg cgagcgcgag caccgcaacg atgatgatgc gggaccgtct
    23521 catgattcct cccctactcc cccaggtacg ccttgcggac ctcgtcgttg ccgaggagct
    23581 tgtccgcccg atcgctcaga cggatctcgc ccgtctcgat cacgtagccg cggtgcgcgg
    23641 tctgcaacgc catctgggcg ttctgctcga tgagcaggat cgaggttccc tgctggttga
    23701 tctcggtgat gatctgaaag atgcgttcga cgaacatcgg ggccaggccc atcgagggct
    23761 cgtcgagcat cagcagcttg gggcggccca tcagcgcgcg gcccatggcg agcatctgct
    23821 gctcaccgcc ggacagcgtg ccgccggcct gcttgatgcg ctctttcagc acggggaata
    23881 gggtgaacac gcgatcgaag tccggggcgt cgggttcgcc gcgatggtag gcgcccatct
    23941 ccaggttctc ccgcacgctc atccccggga aaatgcggcg cccttcgggc gactggatca
    24001 ggccgcgggt gacgatctcg tgcgcggcga gcttgtcgat acgctcgtcc agaaacgaga
    24061 tctttccttt ggacggccgc cgcacgcccg agatggtctt gagagtcgtg gtcttgccgg
    24121 cgccgttgcc gccgatgagc gtgacgatct cgccgggctc gacgaccagc gagattcctt
    24181 tgacggcctc gatgttgccg taggagaccc ggatgtcttc gagcacgagc atcgacatgg
    24241 ctatccagcg tgcccttctg ccgcgccgcg gcccaggtag gcctcgatga ccttggggtc
    24301 gcgctgaacc tgctcgggcg gtccctcggc gatcttctgg ccaaagtcga tcacaaccac
    24361 ctgctcgcag atgcccatga cgaccttcat gtcgtgctcg atcagaagga tcgtgacccc
    24421 gtcgtcgcgg atgctgccga tcagacgcat caacgaggcc ttctcggccg ggttcatgcc
    24481 ggcggccggt tcgtcgagca gcagcagctt gggccgggtc gcgagcgcgc gcgcgatctc
    24541 gacgcgtcgc tggtcgccgt aggacaggtt cttggcggtg tgcgaggcga gatgaacgat
    24601 gccgacgcgc tcgagcatct tgtagccggc atcgatgctc atgtgctcgc tgcggcgcgc
    24661 gagcccgaac atagcggcgc cgaccgtggt gtggctgtgc acgtccgcgc cgaccatcac
    24721 gttctcgatc gcgctcatgt tggggaacaa ccggatgttt tggaacgtgc gcgcgatgcc
    24781 ggccttggtg atctggtgcg gcttcttgcc gcgatacgag ctgccgtcga agcgcacctc
    24841 gccctcggtc ggcttgtaga cgccggtgac gacattgaag aacgtggtct tgcccgcgcc
    24901 gttcggaccg atcaggcccc ggatctcgcc ctcgttgacg acgaacgaca gcgagtcgat
    24961 ggccgtcagg ccgccgaagc gcatcgtgat ggcatcgacc tcgagcaggc tcattcgtcg
    25021 cctcccgcgg gagccggctc ggccggcaga ccgccgccgg tcgggccctt ggtctcttcc
    25081 gcgccgccct tcatctcggc cgcgcgacgc cggctgggca cgatgccctc gggccggaag
    25141 atcatcatga tcaccaagac cagcccgaag atgccgaacc gggcgtcgag cagcttgtcc
    25201 ccgatagtgc cgagcttgct cagcccgcgc agcatctccg gcagcacgtt gatcagcacg
    25261 gctcccacca taacgcccgc ggtcgatccg agcccgccga gcaccacggc cgacaggatg
    25321 aagatcgaga tgatcagatg aaacgacacg ggatcgataa acgtgacctt tgccgcgaac
    25381 accatgccgc ccacggatgc gacggcggcg ccgatcgcga acgcccagag cttgtacttc
    25441 agcgtcggaa cgcccatcgc ctcggcggcg acctcgtcct cgcggatggc ggcccacgcc
    25501 cggccgacgc gcgagttgtt gagggatcga atcaagatgg ccgcgacgcc gatgaaaaag
    25561 agcgtcaaaa ggtagtaagg cttgggatcg agccccagcg acttgccgaa catgctgatg
    25621 ttctcgacac cggtgatgcc gcgcgcgccg ttggtgaccc cgtcgaggtt ctgcgccacg
    25681 atgcggacga tctcgccgaa cccgagcgtg acgatggcga gatagtcgcc gcgcagccgc
    25741 aacgtcgggc cgccgaggat gacgccggtg atcatcgcga tgacgatgcc gagcggcagg
    25801 atctgccaaa acccgagatc ccacggaagc tggatcggac ccgcgttgga caagatcgcc
    25861 atggtgtagg cgccgacggc gaagaatgcc acgtagccca ggtcgagcag tccggcgtag
    25921 ccgaccacga cgttgagccc catcgcgagc agcgcgttgt agccgaccag gaagcccagc
    25981 tcgagcttgg cgacgtccag gaatgtggtc agcagcgcga agacgccgaa gaaggccgcg
    26041 aacacgaccg ggaacatcca cttctcgcgc ttgtcgtaga actcgcgaac gcggccgccg
    26101 accttggagt cgccgtaggc ccacgcgaag atgatcgcga tcacgctcgc caacgccagc
    26161 agcggccgca ttacgccttg ccctccacga cgtcctcacc caggatgccg gtcggacgga
    26221 acagcagcac cagcaccagc acggtaaagg cgatgacgtc cttccatgcg tcaccgaagc
    26281 aaccggcgcc gacgttttcg atcaggccca gcgtcaggcc gcccagcagg gcgccgcgaa
    26341 tgtttccgat gccgcccaga acggcggcgg tgaaggcctt gataccgggc aggaacccga
    26401 cgtcgaaccg ggtcgggaac cgcagccccc acaggagcgc ggcgacgccg gcgagcgcgc
    26461 cgccgatgat gaaggtccag gtgacgaccc ggtcgatgtt gacgcccatc atcgaggcgg
    26521 tctcggcgtc ctgtgcgacc gcgcggatgc ccttgccgat ccgcgtgcgc tgcaccagga
    26581 agtccagccc cacgaccatc acgatcatcg cgatgaacac gatgatgtcc accgtgctga
    26641 tctggacccg gccgatctcg atcagcgggc ggatggggat cgtctcggga aactgctcga
    26701 agctgcggcc tcgccagatc agatagatgc tttgcatgaa cagcgaggca ccgatcgcgc
    26761 tgatcaggaa cgacagccgg gaggcgccgt gtcggcgcag cggccggtac gcgcctcgtt
    26821 ccagcgcgac cgcgacggcg gcgctggcca ggatcgcggg gatggcggcc acgatcaggt
    26881 ggaacgcgtt gccgccgtcg ccggcgatga ggttcagggt gatgagcccg ccgaaggtgc
    26941 ccgcagcgaa gacctcgctg tgggcgaagt tgatcaggcg caggacgccg tagacgaggg
    27001 tgtagccgag tgcgatgagc gcatagatgg aaccggtcgt cagaccaccc accattcgca
    27061 gcgggaggtc tgaggtgatg cactcggagg cgatgcccat tcgggttcgg aggaggggcc
    27121 gcccaacggc ggcccctccg atgcgtcgtg tccggcttta cgccggaacg atgtcgttga
    27181 cgttgccgtc gaggacgatc ttgccttcct tgaccgagta gatgttgacg cccttgtccg
    27241 tgacctcgtg cagggcttcc ttgtcggtcg tccaattgaa ggtcttcgtc aggcccttga
    27301 agtccttcag gttggtgatc ttgtccagca cgcaggcccg cgtgacgtcg gtgccgcagt
    27361 ctccgatcgc cttgatgaag atctgcgcga cgtcgtaggc ctccggagcg tagatgtccg
    27421 ggtccgtgtt gaacttcgcc ttgtagtccg tcacgaactg cttcgcctcg gcctcgtcgg
    27481 atacgttggc gtccaggcac gggcacgtca tgagcgtgcc ttcggcgttg gaggcgcccg
    27541 cctcggggat gtacttggtg gacttggttc cgtcacccga gatgaactgc gcggcgacgt
    27601 tcttttgtcg catctgcttg atcaggatcg acgactgcgg ttggtagccg ccgtagacga
    27661 cgcacttcgg gctggacgag cggagcgtgt ccaccagcga ggtgtagtcg gtcgtcggcg
    27721 cgacgctctc gcgcgtgacg acgcggccgc cgtactcctt gaccttggcg tccacgatgt
    27781 cggcgtagcc cttgccgtag gcggacttgt cgtcgatcac ggcgacggtg tcgcacttgg
    27841 gcgcaccctt gacggcgatg cgcgccgcgg cctcggcctg gtcggtgtcg ccaccgaccg
    27901 aacggaagaa cgtcgggaac ttgccgccgg ccgtcaggtc ggcacgggtg gccgactgcg
    27961 tgacgtgggt cagaccggct tgcgcgaaca gcggctggac cgcggaggtc tcaccggaga
    28021 acgccggacc gatcacgccc acgacggcct gatcgccgat cagctgctgc gcgagcggcg
    28081 gggccttgga cgggtcggct tccttgttgt cgagcgggac gaactcgatg gtgaccttca
    28141 ggtcgccctt ctggttcgct tgctcgaatg cgagctgggc ggcgttacgt cccgggacga
    28201 cgagtgcgga gttcgcaccg gtcagatcgc cgatgaatcc gatcttgacg gtcttcttcg
    28261 acgagtcggg tccttgaccc gttccgccgt cgtcgccgcc acaggcggcg gcgaccagcg
    28321 tgagcgccac gagagccgcg gcgatcctca cgattcgctt cctcacttcg aatccccctt
    28381 ccggccgggg acgccagggg gtccccaatc cattggatac ggcacaatcg aaccttgcgg
    28441 gataataccc cgcttgcctc gtcgggccta ggctaacggc gaccgcctcc ttcggccgcg
    28501 aagacgggag cctcctccca caagggtcta cggggatgac tgccccggag gatggcgagc
    28561 tacgcgtccg cgcccagtga accgtcgagg atccggtccg cgacctcgcg catgcccagg
    28621 cgccgttcca tcgaggcttt ctggatgaac cggaacgcat cggcctcggt catggcgaac
    28681 ttgtccataa gcgcgccttt tgcgcggtcg atgaccttgc gggcctcgag ccgctcggtc
    28741 agatccccga cctcgtcggc cagggccgtc agctgctggt accgcccttt ggcgatctcg
    28801 atggccggga gcagatcctg cttcgtgaac ggcttcacca ggtagcccat cgcgccggcc
    28861 tcggcggcct gctcgaccag ctcccgctgc gagtaggcgg tcaggatcag gcagggggcg
    28921 atgcgctcct cgctgatcgc gcgcgcggcc tcgagcccgt ccatgaccgg catcttgatg
    28981 tcgaggatga ccagcccggg gccggtttcg cgcgccaggc gcaccgcggc ttcgccgtcg
    29041 ccggcttcga cgacctcgta gccttcttct tcgagcatct cgcgcagatc catgcggatg
    29101 atggactcgt cttcggcgat gagcacacgt tcggccatgg cgagcgcgag tgtaccccgg
    29161 acgggcgttt cgggccgaac cgcctcagcg ctgctcgaac atgcgctcga gcagcccgtc
    29221 ctgctcggcg cgcagccgct cgagccgctc cttggcctcg ctcagcgacg cctgggtgcg
    29281 ctccgcgtcc tcgcgggccg tgcgatattc gcggtcggcg agcggggtct cggatacgag
    29341 ggcgcggatg cgcgcgtcct cggccgcttc cgacacgaag gcatgctgct cctcgaggat
    29401 ggcgatctcg gtacggagcc ttccgatctc gtcgctcaga gccgtcagtc gctctccgat
    29461 gcccatgacc tgtgctttcg atttgggaag ggatggaagc gtaacgccgc cttcgcggcc
    29521 tggcaacgac gcgcggggct caggcctcgg tccgggcgaa ctgggtctcg tagagctcgg
    29581 agtacaggcc gcccagcgcg agcagcttgg cgtgggagcc gcgctcgcgg atgcgtccgc
    29641 cgtcgatgca cacgatctcg tcggcgccga ccacggtgga caggcgatgg gcgatgacga
    29701 tggaggtccg gccttccagc gcgacctcga gggcctgctg gatgagcacc tcggactccg
    29761 agtccagatg cgaggtcgcc tcgtccagga tcacgatggc cgggtccttc agcagcagcc
    29821 gcgcgatcgc gagccgctgc ttctcgccgc cggacagccg gtagcctcgc tcccccacga
    29881 ccgtgtcgta gccgtcgggc agcgaagcga tcaggtcgtg gatgcgggcc gccttggacg
    29941 cggcaacgat ctcgtcgtcg gaggcgccgg gccgcgcgaa gcgcaggttg ttgccgatcg
    30001 agtcgtggaa caggtgcggg tcctgcgaga cgatgccgat cgcggcgtgc agcgactcga
    30061 gcgtcacgtc gcgcacgtcg cggccgtcga tgcgcacggc gccgtcggtg acgtcgtaga
    30121 tgcgggcgac gagcatcgcg gtggtggtct tgcccgcgcc cgacgggccg acgagcgcca
    30181 cggtgcggcc cggctcgacc gtgaagctca tctcgcggag cacgagctcg gggatcgcgt
    30241 cttcgtcctg ggcgggcagg ccttcctcga gcgaggcgag cccgacctcg cgtccggccg
    30301 gatagcggaa cgagacgtgg tcgaagtcga tccggccggc gggccggggc agcacgaccg
    30361 cgccgggccg gtcggcgatg gcgtgcggca ggtcgagcac ctcgaagacg cgctcgaacg
    30421 acacgaaggc ggtcatcagg tccacgcgcg agttggtcag ctgcgacagc ggcatgtaca
    30481 gctgcgcgac gtaggcggcg aaggcggcca gcgtcccgat cgtgatggtg tcgctgagca
    30541 ccatccgacc gccgacgtag tacacgaccg cggtgccgac ggccgcgacc aggccgagca
    30601 gcacgaagaa gatgcgcatg aacagcgcgg tgcgaatgcc gatgtcacgg acgcggccgg
    30661 cgcgctcgga gaaggtggtc ttctcctcgt cggggcggcc gaagagcttg gccacgagcg
    30721 cgcccgagac gttgaaccgc tcggtcatca cggtgttcat ctgtgcgttg agcgccatct
    30781 gctcgcgtgt gatcacctgc agccgccggc cgaggcgctt ggacgggatc aagaacgcgg
    30841 gcagcacgat cagcgcgagc aacgtcagcc gccactcgag cgcgagcatg accaccagga
    30901 tcgacacgac cgcgatcgtg ttctgaacga tcgtcccgag cgtgccggtt accgcttgtt
    30961 gcgcgccgac gacgtcgttg ttgagccggc tgatcagcgc gccggtctgc gcgcgcgtga
    31021 agaacgcgag cggcatgcgc tggacgtgat cgaacagccg gacgcgaaga tcgaagatga
    31081 ggcc
//
LOCUS       DATLFO010000194        13516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_73772, whole genome shotgun sequence.
ACCESSION   DATLFO010000194 DATLFO010000000
VERSION     DATLFO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73772"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VM841_07905"
     CDS             complement(<1..866)
                     /locus_tag="VM841_07905"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HVL90141.1"
                     /translation="MRGLRVRLIGLAYLIMWRITRWMPERWAVGMFRWGFDRSWRKNE
                     TQRAIVTENLRPIVGDGPGLAPAVRAAFHSYGRYWAEAFRLQDMSDAELESRIDFVGQ
                     EILDEVMAAGGAVLGVPHLGNWDAAGRYVAKRWKLAAVVEVLRPKALFERFVAYRRAL
                     GMTIIPLHRGEEVTRRCVEHIKATGDLLALVCDRDLSKRGIPVRMFGRTTSMPAGPAV
                     VALRAGVPLIPGATWQAPDGRWRFRAFPPIATGTEPETPEEIRRIMQKLATVFEDILR
                     EEPEQWHAFVPY"
     gene            complement(866..1435)
                     /locus_tag="VM841_07910"
     CDS             complement(866..1435)
                     /locus_tag="VM841_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90142.1"
                     /translation="MNAENRHDPVTRNRLRASAARPSDRAPAGIWLGSGLAAMALAGV
                     FAARSQARPAAAALLAAALLLWLAVARPRQSPFGAYLTALVDQVGDAIVLAPLAWPLA
                     HGSVQEGAPAVSALGLVFVGAYAQVRSRALGYDTPAAPDGAPERSAVLAVGLLAPATF
                     LEPALWIVGALGAAAAARVTVVVWKARGT"
     gene            1560..3641
                     /gene="fusA"
                     /locus_tag="VM841_07915"
     CDS             1560..3641
                     /gene="fusA"
                     /locus_tag="VM841_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVL90143.1"
                     /translation="MKSYPTDKIRNVILVGHQGAGKTSLAEALLFASGTINRPGRVED
                     GNTVTDFDPDEVKRQISVSLGLAPFEWHDHKINLLDAPGYADFVGDLEAGMRAADAAI
                     IVVSAVDGVEVQAQAAWDIAERIGLPRAIFINKLDRERASFDRTLDELTELFGTKIAP
                     VHLPVGEEHDFHGVVDLLDEKAFFYDEGTRHEEPIPDELADREHKLHEALVESVVVAD
                     DSLMERYLEGDPIQRKEVAATLSKAIEECSSVPVLCGSATKGIGVDLLAEFFVEELPS
                     PADRPPRKATIKGGETIELAPTTESPLSAVVFKTLADPFVGKITFFRVFSGSLRPDAP
                     LYNATRDLEERIGQVFTMRGKTQENMSEIGCGDIGAIAKLNDTLTGDTISNKAQPLTL
                     KAWDIPDPLYSVAIAPKAQGDEDKMSSALHKLEVEDPTFRWRRDPETHQTVIQGMGET
                     HVDVMIERLHRHHVEVETLPLKIPYRETIRGSASATGQLKKQSGGRGQYAVASLDVSP
                     LPRGSGFEFEDAIVGGAIPNNFIPSVEKGCRKTLEDGVLAGYPVIDVKARLFDGKFHS
                     VDSSDIAFQIAGGQAFKDACLAAGVVLLEPVVDLIVTIPDDFTGDIMGDLSSRRARIE
                     GTESVGKGWNQIKAKVPQAEVVRYAIDLRSMTGGRGAFSVAPSHYDPCPPNVQEKVVA
                     EAAKMKEEASK"
     gene            3662..4444
                     /locus_tag="VM841_07920"
     CDS             3662..4444
                     /locus_tag="VM841_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90144.1"
                     /translation="MKRLVLAALCAAFLLPLLPAAPAFAACTPPPGRSSDEGKARIRS
                     YPIVVYGAIGGDVPGDAHGAYSLFFDVIRWYKGSDPQGRRRLTVTNYADLELHDGFED
                     PGNRESIAGSVEFLRRFAGQRAVLFLTPRDDALGRDTQGRYKDQYSTTSCTYNVVGNK
                     DVPALLRLLALVFREPVPVSPGATIGPGTPGPGDPSAIPTGSLEPRAAGETAEEEGRS
                     GPITRAAGLVVLVALALVLYRSNRSPLFEQWRARHSDRTKGS"
     gene            complement(4764..5249)
                     /locus_tag="VM841_07925"
     CDS             complement(4764..5249)
                     /locus_tag="VM841_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HVL90145.1"
                     /translation="MERLWTPWRMEYIKDPGREDVCIFCEIPKREDDDAGLILARGSL
                     AFVILNKYPYNTGHLMIVPFRHVGEWESMSADEHAEIAELTGRAMEALKASSAPHGFN
                     LGVNQGRVAGAGVVDHVHMHVVPRWGGDTNFTFTVGGVKVLPEALDDTYAKLKPHFAG
                     R"
     gene            complement(5249..7036)
                     /gene="thrS"
                     /locus_tag="VM841_07930"
     CDS             complement(5249..7036)
                     /gene="thrS"
                     /locus_tag="VM841_07930"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HVL90146.1"
                     /translation="MKLHDGTEIAADSSDGREILRHSTAHIMAQAVCELFPGARYAIG
                     PPIEDGFYYDFDIGRPFTPEDLAKIERKMRGIMKQSQPFEREELTKDEGLKLFADQPF
                     KREIIEGIGGDEDAAEGGGSDVVTVFRNKSWSDLCRGPHVASTGDIPAFKLLRIAGAY
                     WRGDEKRPMLQRIYGTAWESQDALDAHLHKLEEAARRDHRKLGRELDLFSFPEELGGG
                     LLVWHPKGGVLRKQLEDYAREVNLAAGYVPVYSPHVARSVLWETSGHLRQYRDNMYPG
                     MELDAHEYFVKPMNCPFHVLIFGSKTRSYRDLPIRMFELGTVYRYERSGVLHGLMRVR
                     GLTQDDSHIFCRPDQLVDEIVRVMDLTVEIHGTLGFGEPVIHLSTRPGKAIGDPEMWE
                     RATDALKGALDRWGKPYEIAEGEGAFYGPKIDIHFHDAIGRLWQLTTIQVDFALPEAF
                     DLSYAGDDNAKHRPVMIHRAIYGSVERVTGVLIEHYAGAFPAWLAPVQAVVVPIADRH
                     NDAAQALGDRLSAAGVRVEVDLSDGTMQNKVRNAEMQKTPYVLVLGDKEIEGGTLAVR
                     KRGAGRPQFGVPAEEFIAGLLSEIETRAH"
     gene            complement(7056..8306)
                     /locus_tag="VM841_07935"
     CDS             complement(7056..8306)
                     /locus_tag="VM841_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90147.1"
                     /translation="MTRRLRPVAVALAVVIGALGPAAPAQVPEATAAIQAMLDERARA
                     LRDGDLAAFDRTMAGGEPEFVARERAGFRRLRTLPLGEYRIEIGDEEFGDLARDRDRV
                     PGFEETRILQASERIAFRGFDTHPEKEDVFLTVVRKGSDWTVYDDEGVEDLGLKSVRH
                     LWDFGEIETRRSDGIVVVFHPSERAAADPVLEMSRSARARARSGWPMPWDDPIVVMIP
                     STVGELERILQTTFDLTEFVAFAASSVDRDGGYRLTGHRIYLHWPNFRRYQAGFQRQV
                     LAHEFVHLATRAIAGPYITAIFDEGIAQVYGEDGGETTRIARRVRANTLPGTLVPDWY
                     FTAGPRDDIFLAYEEAASFIGYLNGRFGRDAGARAYKALGTIDPMTHGTWRYHLDRVT
                     VSLFQESFPALERAWLDSVRKRFS"
     gene            8401..8709
                     /locus_tag="VM841_07940"
     CDS             8401..8709
                     /locus_tag="VM841_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL90148.1"
                     /translation="MKVLAKAAEIQGVMLLSIDQVAERAGVHPSTLRRWLKAGGHLPK
                     PLPVKALGGGPLWRESEIDRWWQSQVRGGEAFAGLPKAEVAHAVGESPAPVARLRIVR
                     "
     gene            complement(8731..8802)
                     /locus_tag="VM841_07945"
     tRNA            complement(8731..8802)
                     /locus_tag="VM841_07945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8768..8770),aa:Val,seq:cac)
     gene            complement(8815..12120)
                     /locus_tag="VM841_07950"
     CDS             complement(8815..12120)
                     /locus_tag="VM841_07950"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL90149.1"
                     /translation="MTLPGEGSGRAEDVQEPERRLVSVVFADLVGFTAASGERDPEEV
                     HALLWPVMQRLEQIVESFGGRVVKTMGDGLMSVFGVPAASPEDAERAVRAAVAMRDEV
                     HASAGAGSLVSDLSAGVHTGWVFVVPARGGFDVIGDTVNVASRLVGLAGAGSVLVGAS
                     TAQRTSSVIAYREREPAFVKGKPEPIVHFEVAEAGSFGSSGGLAPPAGPFVGRVAELA
                     ALRQEWDAARVERRVRVVSVLGPPGIGKTRLVSEFVRTVEGAPVLVGRAAPHAERIPW
                     FALADALRGADVAREALRLIEGRQPVSPLHRDDRNEMNSEIAAAVRTALSDLTRRDAA
                     VLILEDVQWADPQLGALLADVAAHPWDVPLLILCPGRADTAVHRPAAAAGRVIRLENL
                     DRSETQGLLRKLLPGATIGADVEEELIARAGGNPLFIEEIIRLFRETGVLHRDAGCWR
                     LSEGHRAELPESVQLVVAARIDVLPADERRLIRDAAVCGDTFRLGDLRALGWTDPGDR
                     LDDLVGRDLLVHAGGGYAFRHAVIRDVAYAALPRAERARRHLALAAAIQKVCGPDALP
                     VETLAHHYAQAAVATASSQSPADPSVVSAALEYLVRAGDHALTIRAFREAEAWYRQAL
                     EIHEPSAEAASTPHGRASVRASLKHSAALIELHRYEEATAEASRTLEIARAGGSRPWE
                     AEAVLQLGWLESEYGNLERARRLLDRAGEHFIALGDSHGRARVVWAAARTMRLTDVDE
                     MLSRFEQAAVLFAEAGDAIAESLLYEQLAYGCSARGGEQFRRWYERCSRAAALAGDPR
                     ARAALLRTDGFHRFYRGTWAEAQDPLRRALTIARETGDFFVECDALYLLARIAVGRGE
                     LDEAAAAASSLLAAAGDLAAHRQAEQAHCALARVAARRGDAAAAERHLEEAEAVLAGA
                     GNEREMQEVVLARADIALDGGRWDDAITEAARYRERAERFGDRLEIPVPMLIAGRALL
                     GAGRLEEAAAELDRALKAAEEAGNARVASLAAACGAEAAALRGLAAEAAALLDRARTL
                     GPDPSRMLPDEAAAEAEAAAMVVAAEDRPWTDLRAEAVRAWRTLGVTVWAARAASLQP
                     HPEPAVHGDVGTL"
     gene            complement(12117..12710)
                     /locus_tag="VM841_07955"
     CDS             complement(12117..12710)
                     /locus_tag="VM841_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90150.1"
                     /translation="MNLYAFVSTIPGTASFVAAELVRIGAERAVEVTGLYDVAARFVD
                     QTWESWAGIKNGASQIAGVTSIREGIVVDPGAVGVVSGRMPFSDPRWGPSGLVLLHVA
                     PADGEAVWERLRAAKRGGAVKAMVGLLGAHDIMIQVAGKDESAIAATTMEVLRDIPEI
                     RDATTCLILRGVPAANYPAPKASRAPRRTTRSAKAKR"
     gene            complement(12911..13147)
                     /locus_tag="VM841_07960"
     CDS             complement(12911..13147)
                     /locus_tag="VM841_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90151.1"
                     /translation="MATTQDPNATPGTLKAALVLWMALMAGLLGYSIATADPNAADDH
                     KKSESSESHGEEKPAGDAGHDEPAASPEGEASPH"
     gene            13232..>13516
                     /locus_tag="VM841_07965"
     CDS             13232..>13516
                     /locus_tag="VM841_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90152.1"
                     /translation="MRKAFALIVLVAAIFGNRAQASSLVVTQRITAPEADRPAHFFGG
                     SIAAHGRWAVTGSTQDREGGDRAGAVYVMRRTAGEWALTQKLLAPDAADEM"
BASE COUNT         2076 a         4784 c         4636 g         2020 t
ORIGIN      
        1 cagtagggaa cgaacgcgtg ccactgctca ggctcttccc gcaggatgtc ctcgaagacg
       61 gtggcaagct tttgcatgat gcgccggatc tcttcggggg tctcgggctc ggtgcccgtg
      121 gcgatcggcg ggaaggcgcg gaagcgccag cggccgtcgg gcgcctgcca ggtcgccccc
      181 gggatcagcg ggacgcccgc gcgaagcgcg accaccgccg ggcccgccgg catggatgtg
      241 gtccgtccga acatgcgaac cggaatgccg cgcttggaca ggtcccggtc gcacacgagc
      301 gccagcaggt ctccggtcgc cttgatgtgc tcgacgcatc gacgagtgac ctcctcgccg
      361 cgatgcagcg ggatgatggt catgccgagc gcacgccggt aggcgacgaa gcgctcgaac
      421 agcgccttgg gccggagcac ctcgacgacc gcggcgagct tccagcgctt ggcgacgtag
      481 cggccggccg cgtcccagtt gccgaggtgc ggcacgccaa ggaccgcgcc gcccgccgcc
      541 atgacctcgt cgaggatctc ctgcccgacg aagtcgatcc ggctctccag ctcggcgtcg
      601 ctcatgtcct gcagccgaaa cgcctcggcc caatagcggc cgtaggagtg gaatgccgcg
      661 cgaaccgccg gcgcaaggcc gggcccgtcg ccgacgatgg gccgcagatt ctcggtgacg
      721 atcgcgcgct gcgtttcgtt ctttcgccac gaccggtcga agccccaccg gaacatgccg
      781 accgcccagc gctcgggcat ccatcgcgtg atgcgccaca tgatcagata ggccaaaccg
      841 atcaggcgca cgcgcagccc gcgcatcagg tgccccgcgc cttccagacc acgaccgtca
      901 cgcgtgcggc cgccgcggcg ccgagcgcac ccacgatcca caacgcgggc tccaggaacg
      961 tggccggggc cagcaggccc accgcgagca cggcgctgcg ctcgggcgcg ccgtccggag
     1021 cggcgggcgt gtcgtagccg agcgcccggc tgcgcacctg cgcgtaggcg ccgacgaaca
     1081 ccaggccgag cgccgaaacg gccggcgcgc cctcctgaac gctgccgtgc gccagaggcc
     1141 aggccagcgg ggcgagcacg atcgcgtcgc cgacctgatc gaccagcgcg gtgaggtagg
     1201 cgccgaacgg gctctggcgc gggcgggcca ccgcgagcca cagcagcagc gccgccgcga
     1261 gcagcgccgc cgccgcgggc cgcgcctggc tgcgcgccgc gaacacgccg gcaagggcca
     1321 tcgcggccag gccgctgccc agccagatcc ccgccggcgc gcgatccgag ggccgcgccg
     1381 cggacgcgcg tagccggttg cgggtgacgg gatcgtgtct gttctcggcg ttcacgcggg
     1441 ggaagcatac cgacggcgaa ccccgtcggc gccgggccgc gcgcagggga tgcagcccgc
     1501 ggccgcgaaa acccctgggg ctatactttg gcctctcgtc actatcggag gttgccgacg
     1561 tgaagagcta cccgaccgac aagattcgca acgtcatcct cgtcggacat cagggcgcag
     1621 gaaagacgtc gctcgccgag gcgctgctct tcgcctccgg caccatcaac cgtcccggcc
     1681 gcgtcgagga cggaaacacc gtcaccgact tcgaccccga cgaagtcaag cgccagatct
     1741 ccgtctcgct gggcctcgcg ccgttcgaat ggcacgacca caagattaac ctgctggatg
     1801 cgcccggcta tgccgacttc gtcggcgatc tcgaggccgg catgcgggcc gcggacgcgg
     1861 cgatcatcgt cgtctcagcg gtggacggcg tggaggtcca ggcccaggcg gcctgggaca
     1921 tcgccgagcg gatcggcctg ccccgcgcga tcttcatcaa caagctcgac cgcgaacgcg
     1981 cctcgttcga ccgcacactc gacgagctca ccgagctgtt cggcaccaag atcgctcccg
     2041 ttcaccttcc ggtgggtgag gagcacgact tccacggcgt ggtggatctg ctggacgaga
     2101 aggccttctt ctacgacgaa ggcacccgcc acgaagagcc gatccccgac gagctcgccg
     2161 accgcgagca caagctgcac gaagcgctcg tcgagtccgt ggtggtcgcc gacgactcgc
     2221 tgatggagcg ttacctggag ggtgacccca tccaacgcaa ggaggtcgcc gcgaccctgt
     2281 cgaaggcgat cgaggagtgc tcgagcgttc cggtgctgtg cggatcggcg accaagggca
     2341 tcggcgtgga ccttctcgcc gagttcttcg tcgaggagct tccctccccc gccgaccggc
     2401 cgccgcgcaa ggccaccatc aagggcggcg agacgatcga gctcgcgccc acgacggagt
     2461 cgccgctcag cgccgtggtg ttcaaaacgc tggcggatcc gttcgtcggc aagatcacct
     2521 tcttccgcgt cttctcagga tcgctgcgac ccgacgcgcc cctctacaac gcgacacgcg
     2581 acctggagga gcgcatcggc caggtcttca cgatgcgcgg caagacgcag gagaacatga
     2641 gcgagatcgg ctgcggcgac atcggcgcga tcgccaagct caacgacacg ctcacgggcg
     2701 acaccatctc gaacaaggcg cagccgctga cgctgaaggc ctgggacatc ccggatccgc
     2761 tctactccgt cgcgatcgcg ccgaaggcgc agggcgacga ggacaagatg tcgtcggcgc
     2821 tgcacaagct cgaagtcgag gatcccacct tccggtggcg tcgcgatccc gagacgcacc
     2881 agaccgtcat ccagggtatg ggcgagacgc acgtggacgt gatgatcgag cgtctgcacc
     2941 gccatcacgt cgaggtcgag acgctgccgc tcaagatccc gtaccgcgag acgatccgcg
     3001 gttcggcttc ggcgacgggc cagctcaaga agcagtcggg cgggcggggc cagtacgcgg
     3061 tcgcctcgct cgatgtctcg ccgttgccgc gcggctccgg tttcgagttc gaggacgcaa
     3121 tcgtcggcgg cgcgatcccg aacaacttca tcccgtcggt cgagaaagga tgccgaaaga
     3181 cgctcgagga cggggtgctc gccggctacc cggtcatcga cgtgaaggcg cgcctgttcg
     3241 acggcaagtt ccactcggtg gactcgtccg acatcgcctt ccagatcgcc ggcggccagg
     3301 cgttcaagga cgcgtgcctg gcggccgggg tcgtgctgct cgagccggtc gtggatctga
     3361 tcgtgaccat cccggacgac ttcacgggcg atatcatggg cgatctgtcg tcgcggcgcg
     3421 ctcgcatcga aggaaccgaa tcggtcggca agggctggaa ccagatcaag gcgaaggttc
     3481 cgcaggccga ggtggtccgg tatgcgatcg acctgcggtc gatgaccggg ggacgaggcg
     3541 cgttctcggt tgcgccgtcg cactacgatc cgtgtccgcc caacgtccag gagaaggtcg
     3601 tggccgaagc agccaagatg aaggaagagg cgagcaagta gcggacgcgt ccgcgcgacc
     3661 tatgaagcgt ctcgtccttg ctgcgctctg cgccgctttc ctgttgccgc tgctgcccgc
     3721 cgcgccggcc ttcgcggcct gcacgccgcc gccgggtcgc tcctccgacg agggcaaggc
     3781 ccgcatccga tcgtatccga tcgtggtgta cggcgcgatc ggcggcgacg tgccgggcga
     3841 tgcgcacggc gcgtactcgc tcttcttcga cgtgatccgc tggtacaaag gctccgaccc
     3901 gcagggccgg cggcgcctca ccgtgacgaa ctacgccgac ctcgagctgc acgacggatt
     3961 cgaggacccg ggcaaccgcg agtcgatcgc cggcagcgtc gaattcctgc gtcgcttcgc
     4021 cggccagcgg gcggtgctgt tcctgacgcc gcgcgacgac gcgctcggcc gcgacacgca
     4081 aggacgctac aaggaccagt actcgacgac ctcgtgcacc tacaacgtgg tcggcaacaa
     4141 ggacgtgccg gcgctgctgc ggctcttggc cctggtcttc cgcgagcccg ttcccgtgtc
     4201 accgggcgcc accatcggtc cgggcacacc cggtccgggc gatccgagcg cgattccgac
     4261 cggatcgctc gagccccgcg ccgcgggcga gacggccgag gaggagggcc gctccggccc
     4321 gatcacacgc gcggccgggc tggtcgtgct cgtcgcgctc gcgctggttc tgtatcggag
     4381 caatcgctct ccgctgttcg agcagtggcg cgcacggcat tcggaccgca cgaaaggttc
     4441 ctagacgcgg agaaagggct gacagaggtg gcaacccgtc aaccctttct cacttcggtt
     4501 gtggctccct ccccgcatcc gaagggaccg acatcgtcga tctcgccctc gggcggaggg
     4561 tcaccctagc cggtcggctc ggcgcggcag cccaccaggg gcacagtgcg gggcgctcgc
     4621 tcgttcggcc ccgccggaag ccacgcttgg aacttgtccg gcagtgcgat tctgcgccga
     4681 cgcgccgcgg atgtctaggg ggtggacgca actttttcgc aacaccagca acatccgcag
     4741 cggcgtgtcc caattgttca agatcagcga ccggcgaagt gcggcttgag cttggcgtag
     4801 gtgtcgtcga gcgcttccgg aagcaccttc acgcccccga cggtgaacgt gaagttggtg
     4861 tcgccgcccc atcgcgggac gacgtgcatg tgcacgtgat cgacgacacc cgcgccggcg
     4921 acgcggcctt ggttgacgcc gaggttgaat ccgtgcggcg cgctacttgc cttgagtgct
     4981 tccatcgccc ggcccgtcag ttccgcgatc tcggcgtgct cgtcggcgct catcgactcc
     5041 cattcgccga cgtggcggaa cggcacgatc atcagatggc cggtgttgta cgggtacttg
     5101 ttcaggatca cgaaggcgag cgagccgcga gcaagaatca gcccggcatc gtcgtcttcc
     5161 cgcttgggga tctcgcagaa gatgcagacg tcctcgcggc cgggatcctt gatgtattcc
     5221 atgcgccacg gagtccagag ccgttccatc agtgggcgcg ggtctcgatc tcggagagca
     5281 ggccggcgat gaactcctcg gccggcacac cgaactgcgg cctgccggcg cctcgcttgc
     5341 ggaccgccag cgtcccgccc tcgatctcct tgtcgccgag caccagcacg tacggcgtct
     5401 tctgcatctc ggcgttgcgg accttgttct gcatcgttcc gtccgaaagg tccacctcga
     5461 cacgcacgcc ggcggccgac aaccggtcgc cgagagcctg ggcggcgtcg ttgtgccggt
     5521 cggcgatggg aaccacgacc gcctgcacgg gagcgagcca cgcggggaac gcgcccgcgt
     5581 agtgctcgat cagcacgccg gtgactcgct cgaccgagcc gtagatcgcg cggtggatca
     5641 tcacgggccg gtgcttggcg ttgtcgtcgc ccgcgtagga caagtcgaac gcctcgggca
     5701 gcgcgaagtc cacctggatc gtggtgagct gccacagccg tccgatggcg tcgtggaagt
     5761 ggatgtcgat cttgggcccg tagaaggcgc cttcgccttc ggcgatctcg tagggcttgc
     5821 cccaccggtc cagcgcgccc ttgagcgcgt cggtcgcgcg ctcccacatc tcgggatcgc
     5881 cgatcgcttt gccgggccgg gtggacagat ggatgacggg ctcgccgaag ccgagcgttc
     5941 cgtggatctc gacggtgagg tccatcacac gcacgatctc gtccacgagc tggtcgggcc
     6001 ggcagaagat gtgcgaatcg tcctgggtga ggccgcgcac gcgcatcaag ccgtgcagca
     6061 cgccgctccg ctcgtagcgg tagacggtgc cgagctcgaa catccggatc ggcaggtcgc
     6121 gatacgagcg ggtcttggat ccgaagatca gcacgtggaa cgggcagttc atcggcttga
     6181 cgaaatactc gtgtgcgtcg agctccatcc cgggatacat gttgtcgcgg tactgccgca
     6241 ggtgaccgga ggtctcccag agcacactgc gcgcgacgtg cggcgagtac acgggcacgt
     6301 agccggcggc gaggttgacc tcgcgtgcgt agtcctcgag ctgcttgcgc agcacgccgc
     6361 ccttcggatg ccacacgagc agcccgccgc cgagctcctc cgggaacgag aacaggtcga
     6421 gctcgcggcc gagcttgcgg tggtcgcgcc gcgcggcctc ctcgagcttg tgcaggtgcg
     6481 cgtccagcgc gtcctgcgac tcccaggcgg tgccgtagat gcgctggagc atcggccgct
     6541 tctcgtcgcc gcgccagtac gcgccggcga tgcgcagcag cttgaacgcg gggatgtcgc
     6601 cggtcgatgc gacgtgcggg ccgcggcaca ggtcggacca gctcttgttg cggaacaccg
     6661 tcaccacatc ggagccgccg ccttcggcgg catcttcgtc gccaccgatg ccttcgatga
     6721 tctcgcgctt gaacggctgg tccgcgaaca gcttcaagcc ttcgtccttg gtcagctcct
     6781 cgcgctcgaa cggctgcgac tgcttcatga tgccgcgcat ttttcgctcg atcttggcga
     6841 gatcctcggg cgtgaacggc cggccgatgt cgaagtcgta gtagaagccg tcctcgatcg
     6901 gcggaccgat cgcgtagcgg gcgccgggga acagctcgca gacggcctgc gccatgatgt
     6961 gcgccgtcga gtgccgcagg atctcgcgtc cgtctgatga gtccgcggcg atctcggttc
     7021 cgtcgtgcag cttcacgtcg tcgctcccct ttgggtcagg aaaatcgctt gcggaccgaa
     7081 tcgagccaag cacgctccag cgcggggaac gactcctgga acagcgaaac ggtcacgcgg
     7141 tccagatgat agcgccaggt cccgtgcgtc atcggatcga tcgtgccgag cgccttgtag
     7201 gcacgcgccc cggcgtcccg tccgaagcgt ccgttcagat agccgatgaa ggacgcggcc
     7261 tcctcgtagg ccaggaagat gtcgtcgcgc ggcccggcgg tgaagtacca gtcgggaacg
     7321 agcgtgccgg gcagcgtgtt ggcgcgcacg cgccgcgcga tccgtgtcgt ctcgccgccg
     7381 tcctcgccgt agacctgcgc gatgccttcg tcgaagatcg ccgtgatgta gggccccgcg
     7441 attgcgcgtg tggcgagatg gacgaactcg tgcgcgagca cttgccgctg gaatccggcc
     7501 tggtagcggc ggaagttcgg ccagtgaagg tagatccggt gcccggtcag ccggtatccg
     7561 ccgtcgcggt ccacgctcga tgcggcgaag gcgacgaatt cggtcaggtc gaaggtcgtc
     7621 tgcagaatgc gctcgagctc gccgaccgtg gacgggatca tcaccacgat cgggtcgtcc
     7681 cacggcatgg gccagcccga gcgagcccgc gcgcgtgcgc tcctcgacat ctcgagcacc
     7741 ggatccgccg cggcacgctc ggacggatgg aacaccacca cgatcccgtc ggaccggcgg
     7801 gtctcgatct cgccgaagtc ccacaggtgc cggacgctct tgagcccgag atcctcgacg
     7861 ccttcgtcgt cgtagaccgt ccagtccgac cccttgcgca ccaccgtcag gaagacgtcc
     7921 tccttttcgg gatgggtgtc gaagccacgg aaggcgattc gctcggacgc ctggaggatg
     7981 cgggtttcct caaagcccgg cacccggtcg cggtcgcgcg cgaggtcccc gaactcctcg
     8041 tcgccgatct caatgcggta ctccccgagc ggaagggtcc gcagccggcg gaacccggcg
     8101 cgctcacggg cgacgaactc gggctcgccc cccgccatcg tgcgatcgaa ggccgcaagg
     8161 tcgccgtcgc gcagggcacg tgcgcgctcg tccagcatgg cttggatcgc ggccgtcgcc
     8221 tcgggcacct gggcgggtgc cgcaggcccg agcgcgccga tcacgacggc gagcgcgacc
     8281 gcgacaggtc ggaggcggcg tgtcatcgac gcggattcta cggtcggccg ggcgtcgcgt
     8341 ccggatgtgc cggatttaga ccgagacggg ttttcatgcc gcatcacggg ggaatggatt
     8401 gtgaaggtcc tggcgaaggc ggccgagatt cagggagtca tgctgctatc gatcgatcaa
     8461 gtcgccgagc gcgccggggt tcacccgtcg acgctccgcc gctggctcaa ggccggcggc
     8521 caccttccca agcccttgcc cgtgaaagcc ctcggcggcg gcccgctgtg gcgggaatcc
     8581 gagatcgacc gctggtggca gagccaggtt cgcggcggcg aggcgttcgc gggcctgccc
     8641 aaggctgagg tcgcccacgc cgttggggaa tcgcccgcac ccgtggctcg cttgcgcatc
     8701 gtgcgctgaa gctgttcgga tcgctttcgg tgggcgcgga gggttttgaa cccccgacct
     8761 ctgccgtgtg aaggcagcgc tctcccgctg agctacgcgc ccgagagacc cggattatag
     8821 cgtcccgacg tccccgtgga ccgccggctc cgggtgcggc tgcagcgatg ccgcgcgagc
     8881 ggcccacacg gtgacgccca gggtgcgcca cgcccgcacg gcttcggccc gaagatccgt
     8941 ccacggccgg tcctcggccg ccacgaccat cgccgccgct tcggcttcgg ccgccgcctc
     9001 gtcgggcagc atgcgcgacg gatcgggtcc caacgtccgg gcgcgatcca gaagcgcggc
     9061 ggcctccgcc gcgaggccgc gcaacgccgc ggcttcggcg ccgcacgcgg ccgcgagcga
     9121 ggcgacgcgg gcattgcccg cttcctccgc ggccttcagc gcccggtcga gctccgccgc
     9181 cgcctcctcc agccgcccgg cccccagcaa tgcgcggccg gcgatcagca tcggcacggg
     9241 gatctcgagc cggtcgccga agcgctcggc ccgctcccgg taacgcgcgg cctcggtgat
     9301 cgcatcgtcc cagcggccgc cgtcgagcgc gatgtcggct cgcgccagga cgacctcttg
     9361 catctcgcgt tcgtttcccg ctccggcgag cacggcctcg gcctcttcga ggtgccgttc
     9421 cgcggccgcg gcatcacccc ggcgggccgc cacgcgcgcg agcgcgcaat gggcctgctc
     9481 ggcctggcgg tgcgcggcga gatccccggc cgccgccagc agcgacgatg ccgccgccgc
     9541 ggcctcgtcc agctcgccgc ggccgaccgc gatcctggcc agcaggtaga gcgcgtcgca
     9601 ttcgacgaag aagtcgccgg tctcacgcgc gatcgtcagc gcgcggcgaa gcgggtcttg
     9661 ggcctcggcc cacgttccgc ggtagaagcg gtggaacccg tcggtgcgga gcagcgctgc
     9721 gcgggcgcgg ggatcgccgg cgagcgccgc cgcgcggctg catcgctcgt accatcggcg
     9781 gaactgctcg cccccgcgcg cggagcagcc gtacgcgagc tgctcgtaga gcaggctctc
     9841 ggcgatcgca tccccggcct cggcgaacag caccgctgcc tgttcgaacc gcgacagcat
     9901 ctcgtcgacg tcggtcaggc gcatcgtccg ggccgctgcc cacaccacgc gcgcgcggcc
     9961 gtgggaatcg ccgagcgcga tgaagtgctc tccggcccgg tcgagcagcc ggcgcgctcg
    10021 ctcgaggttg ccgtactccg attccagcca gccgagttgg agcacggcct cggcctccca
    10081 cggccggctg ccgccggcac gtgcgatctc gagcgtgcgc gaggcttcgg cggtggcctc
    10141 ttcgtatcgg tgcagctcga tcaacgccgc ggagtgcttc aacgacgcac gcaccgatgc
    10201 gcggccgtgc ggggtggaag ccgcctcggc ggacggctcg tgaatctcga gcgcctgccg
    10261 gtaccaggct tcggcctcgc gaaacgcccg gatcgtcagc gcgtgatcgc cggcgcgaac
    10321 cagatactcg agcgcggccg acacgacgga aggatcggcg ggactttgcg acgacgcggt
    10381 cgcgaccgcc gcttgcgcgt agtgatgcgc gagcgtctcg accggaagcg cgtcgggccc
    10441 gcacaccttt tggatcgcgg cggcgagcgc cagatgccgc cgcgcgcgtt ccgcacgcgg
    10501 cagcgcggca taagcgacat cgcggatcac cgcgtgacgg aaggcgtagc cgccgccggc
    10561 atggacgagg agatctctgc cgacgagatc gtcgagccgg tcgccgggat cggtccatcc
    10621 caacgcgcga aggtcgccga gccggaaggt atcgccgcag acggcggcgt cacggatcag
    10681 ccggcgttcg tcggcgggca aaacgtcgat gcgcgcggcg acgacgagct gaacgctttc
    10741 gggcagctcg gcgcggtgac cctcggacag gcgccagcat ccggcgtcgc gatgcagcac
    10801 gccggtctcg cggaacaggc gaatgatctc ttcgatgaac agtggattgc cgccggcgcg
    10861 cgcgatgagc tcctcctcga cgtcggctcc gatcgtggcg cccggcagca gctttcggag
    10921 caggccttgc gtttctgagc gatcgagatt ctcgagccgg atcacccggc ccgccgccgc
    10981 tgccggccgg tgcacggccg tgtcggcacg gccggggcac aggatcagca acggcacgtc
    11041 ccacggatgc gcagcgacgt cggccagcag cgcgccgagc tgcgggtccg cccactgcac
    11101 gtcttcgagg atcagcaccg ccgcatcccg gcgggtcaga tcgctcagcg ccgtgcgcac
    11161 ggcggcagcg atctcggagt tcatctcgtt gcgatcgtcg cggtgcagcg gagagaccgg
    11221 ctgccgtcct tcgatcaggc gcagcgcctc ccgtgcgacg tcggctccgc gcagcgcgtc
    11281 ggcgagcgcg aaccagggga tgcgttcggc atgcggggcc gcccgcccga ccagcaccgg
    11341 tgcgccctcc acggtgcgca cgaactcaga gacgagacga gtcttgccga tgcccggcgg
    11401 gccgagcacg ctcaccacgc gcacgcgccg ctcgacccgt gccgcatccc actcctgccg
    11461 caatgcggcg agctcggcga cgcgtccgac gaacggtccg gccggcggag ccaggccgcc
    11521 ggacgatccg aacgatccgg cctcggccac ctcgaagtgc acgatgggct cgggcttgcc
    11581 ttttacgaag gcgggctcgc gctcacgata ggcgatcacc gacgacgtgc gctgcgcggt
    11641 ggacgcgccc acgagcaccg agcctgcccc ggcgagcccc acgaggcgcg aggcgacgtt
    11701 gaccgtgtcg ccgatcacgt cgaagccgcc gcgcgccgga acgacgaaga cccatccggt
    11761 atgaacgccg gccgacaggt ccgacacaag cgacccggcg ccggccgagg catgcacctc
    11821 gtctcgcatc gcaacggccg cccgaacggc gcgttcggcg tcctcggggg atgcggcggg
    11881 cacgccgaag accgacatca agccgtcgcc catcgtcttg accacgcggc caccgaagga
    11941 ctcgacgatc tgctcgagcc gctgcatcac cggccacaag agcgcgtgaa cctcctcagg
    12001 atcgcgctct cctgacgcgg cggtgaatcc caccagatcg gcaaacacga cgctgaccag
    12061 ccgccgctcc ggctcttgga cgtcctcggc gcggccgctg ccctcgccag gcagggtcat
    12121 cgcttcgcct tcgcgctcct cgtggtgcgg cgcggggccc gcgacgcttt cggcgcggga
    12181 taattggcgg ccggaacgcc gcgcaggatc aggcaggtcg tcgcgtcacg gatctcgggg
    12241 atgtcgcgaa gcacttccat ggtcgtggcg gcgatcgcgc tttcgtcctt gcccgccacc
    12301 tggatcatga tgtcgtgcgc gccgagcagg ccgaccatcg ccttgaccgc cccgccgcgc
    12361 ttcgccgccc ggaggcgttc ccagaccgcc tcgccgtcgg cgggcgcgac gtgcaacagc
    12421 accaggcccg acggccccca gcggggatcg gagaacggca tccggccgga gacgaccccg
    12481 accgcgcccg ggtcgacgac gatgccctcg cggatcgatg tcacgccggc gatctggctc
    12541 gcgccattct tgatgccggc ccacgactcc caggtctgat ccacgaacct cgccgcgaca
    12601 tcgtagagac cggtgacctc caccgcgcgt tccgcgccga tcctgaccag ctcggcggcc
    12661 acgaacgagg ccgtccccgg aatcgtgctc acgaacgcgt agaggttcat tcagctcccc
    12721 cttcgacgat gcaaagagtg cggctcgtgg atgccttgta ccgcacccgg gaccgggcgc
    12781 gcgaattcac cctgccatcg cgtgatcgcc gtatcgcccg cgtgccggca ggatccgcgg
    12841 gcgtcagagg gccgttctgc gaagcggacc cctcgccgtg cgaggggtcc gtcattcgaa
    12901 cgggcccctg ctagtgaggt gaggcttctc cctcgggcga agccgccggc tcgtcgtgac
    12961 cggcgtcccc ggcgggcttc tcctccccgt ggctctcgga ggactccgac ttcttgtgat
    13021 cgtcggcggc gttcgggtcg gcggtggcga tcgagtaccc gagcaagccg gccatgagcg
    13081 ccatccacag cacgagcgcg gccttgagcg ttcccggggt tgcgtttggg tcttgggtgg
    13141 ttgccatggg cggcatttca gcagagccgc ggctcggatt caaagcggac gcagggcagg
    13201 aaggcgcgca tgtccgcacg aactgaccgg catgcggaaa gcgtttgctc tcatcgtgct
    13261 cgtggccgcg atcttcggaa accgcgccca ggcctcgtcg ctcgtggtga cccagcgcat
    13321 cacggccccg gaagcggacc gtcccgctca cttcttcggt ggatccatcg ccgctcacgg
    13381 gcgatgggcg gtgaccggat cgacccaaga tcgcgagggc ggcgaccggg ccggcgccgt
    13441 ctatgtcatg cgccggacgg ccggcgagtg ggcgctcacg caaaagctgc tggcccccga
    13501 cgccgccgac gagatg
//
LOCUS       DATLFO010000195         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_57546, whole genome shotgun sequence.
ACCESSION   DATLFO010000195 DATLFO010000000
VERSION     DATLFO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57546"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VM841_07970"
     CDS             complement(<1..324)
                     /locus_tag="VM841_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90153.1"
                     /translation="MTAFASRWQHRGDAAPSRVRRIAIRHLVRRLPGAGIRDGFFRDS
                     CGDGDPRRRGGPVTRAFCIAFAVCIAFAVCIAFAVCVAFVLCVALNAGGTGLARLVVA
                     GLRRRR"
     gene            364..2022
                     /locus_tag="VM841_07975"
     CDS             364..2022
                     /locus_tag="VM841_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HVL90154.1"
                     /translation="MRRSACFAAERGTESRIRILFEGGMSLDIRELKGSGRAVRALIS
                     VGTLITWIVGAVAARIFLDMDWDVAILLGAILTVTGPTVIGPLLRHIRPTGLSASVLR
                     WEGILIDPIGAMLAVLVFEATLAGNARIAANIFTQGLLQTIGSGLGIGIGAAALMVVL
                     LRRYLVPDPLHGALALTLVVGAFVAANEIQEESGLLAVTVMGITLASQRLVPVKHILE
                     FEENLRTLLISVLFVTLAARLTRGDLQMLGLGALAFVVSLIVIARPLAAMASLARTKL
                     TWRDRAFVAAMAPRGIVAAAVSTLFALRLEEAGNVDVSLLVPATFAVIVGTIVVYGLS
                     GPWLARRLGLAESKPGGLLIIGAHAGARSLAQALQSKGVRVLLTDTEYGHVSAGRLAG
                     LPVVYGSVLSEDTLATMDFGGIGSMLALTPNDEVNALAAQTLAPVVGRRNVYQLAPKD
                     GTGRGRHALAAHLRGRTLAAPDLTFGAIAARLDDGAGFKVTKLSHEFDLDSFHREHGD
                     GAIPLGSIDSAGRLTLFAAGEKRPVKAGNLLLAIVPARVPERSA"
     gene            complement(2029..>2865)
                     /locus_tag="VM841_07980"
     CDS             complement(2029..>2865)
                     /locus_tag="VM841_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVL90155.1"
                     /translation="RSVTFVLARIVGDGLKLYAIRRSGVLPSSSIFINIARLIVFIVG
                     FLVLLQTFGVSITPMLTALGVGGLAIALALQDTLANLFAGLQLLAAKKVRLGDFIRLD
                     SGDEGEVIDINWRNTSLRQLPNNIVIVPNSRLATAIITNFHMPQTEMAVVFQMGVAYD
                     TDLEHVERVTIEVATETMREVPGAIRTFEPFVRYHTFGDSSINFSVILRCQSYTEQYL
                     LKHEFVKRLHKRYRDEGIEIPFPQRTLHFADPAAEETVRPRGKAQRSASKRPAAKRGS
                     RR"
BASE COUNT          465 a          939 c          992 g          469 t
ORIGIN      
        1 cctgcggcga cggagacccg cgacgacgag gcgggcgagg ccggtgccgc cggcgttgag
       61 ggcgacgcaa aggacgaagg cgacacagac ggcgaaggcg atgcagacgg caaaggcgat
      121 gcagacggcg aaggcgatgc agaaggcacg ggtgactggc ccgcctcgtc gtcgcgggtc
      181 tccgtcgccg caggagtcgc ggaagaagcc gtctcggatg cccgcgccgg ggaggcggcg
      241 aacgagatga cgaatagcga tgcgacgaac acgggaaggc gcggcatcac cgcgatgctg
      301 ccacctggaa gcaaacgcgg tcaacgcaga atgcttgcta aagcaagcaa gttttttctc
      361 cggatgcgcc gatcggcttg cttcgccgcg gagcgcggca ccgaatcgcg gatcaggatc
      421 ctgttcgagg gaggaatgag cctcgacatc cgggagctga agggaagcgg ccgtgccgtc
      481 cgcgcgctca tcagcgtggg cacgctcatc acctggatcg tgggcgcggt cgccgcccgg
      541 atcttcctcg acatggactg ggacgtcgcg atcctgctgg gcgcgatcct gacggtgacc
      601 gggccgaccg tgatcggtcc gctgctgcga catatcaggc caaccggatt gtccgcctcg
      661 gtccttcgat gggaaggcat cctgatcgac ccgatcggcg ccatgctggc ggtcctggtg
      721 ttcgaggcga ccctcgccgg aaatgcgcgg atcgcggcga acatcttcac gcagggcctg
      781 ctgcagacga tcggctccgg gctcgggatc ggaatcggcg ccgcggcgct gatggtcgtt
      841 ctgcttcggc gctacctcgt gcccgacccg ctgcacggcg cgctcgccct cactctggtc
      901 gtcggcgcat tcgttgcggc aaacgagatc caagaggagt cgggcctgct cgcggtgacc
      961 gtcatgggca tcacgctcgc ctcgcaacgg ctcgtgccgg tcaagcacat cctggagttc
     1021 gaggagaacc tgcgaacgct gctgatctcg gtgctgttcg tgacgctcgc ggcacgtctg
     1081 acgcgaggag acctgcagat gctcggcctg ggcgcgctcg cgttcgtggt gtcgctgatc
     1141 gtgatcgcgc gtccgcttgc ggcgatggct tccctggccc gtaccaagct gacctggcgt
     1201 gaccgggcgt ttgtggccgc gatggcgccg cgcggcatcg tggccgcggc cgtgtccacg
     1261 ctgttcgcgc tgcggttgga agaggccggc aacgtcgacg tgtccctgct cgtgccggcg
     1321 accttcgcgg tcatcgtcgg gacgatcgtg gtctacggcc tgagcggtcc gtggctcgcc
     1381 cggcggctcg gtctcgccga atcgaagccg ggcgggctgc tgatcatcgg cgcgcacgca
     1441 ggggcccgct cgctcgctca ggcgctgcaa tccaagggcg ttcgggtgct gctgaccgat
     1501 accgagtacg ggcacgtcag cgcggggcgt ctcgccgggc tgccggtcgt ctacggcagc
     1561 gtcctgtcgg aggacacctt ggcgacgatg gacttcggcg gcatcggatc gatgctggcg
     1621 ctcacaccca acgacgaagt caacgcactc gcggcccaaa cgctcgctcc cgtcgtcgga
     1681 cgccgaaatg tctatcagct cgcacccaag gacgggacag gccgcggacg ccacgcgctg
     1741 gcggcccatc ttcgcggccg cacgctcgcc gcgcccgatc tcaccttcgg tgccatcgcc
     1801 gcgcggctgg atgacggagc gggcttcaag gtcaccaagc tcagccacga attcgaccta
     1861 gactcgttcc accgcgagca cggcgatggc gcgatcccgc tcggatcgat cgattccgcc
     1921 ggccggctga cgctgttcgc ggcgggcgag aaacgccccg tcaaggccgg caaccttctg
     1981 ctggcgatcg ttcccgcgcg cgttcccgag cgcagcgcct agcagcgtct agcggcgcga
     2041 gccgcgcttg gccgcgggcc gcttcgacgc ggatcgctgg gccttgccgc gaggccgcac
     2101 cgtttcctcc gccgccgggt ccgcgaaatg aagcgtccgc tgcgggaagg ggatctcgat
     2161 gccttcgtcc cggtagcgct tgtgcagccg cttcacgaac tcgtgtttga gcaggtactg
     2221 ctccgtgtag gactggcagc gcaggatgac cgagaagttg atgctcgagt cgccgaaggt
     2281 gtggtagcgg acaaacggct cgaacgtgcg gatcgctccc gggacctcgc gcatggtttc
     2341 cgtcgcaacc tcgatcgtga cgcgctcgac gtgctcgagg tcggtgtcgt aggcgacccc
     2401 catctgaaag accaccgcca tctcggtctg cggcatgtgg aaattcgtga tgatagccgt
     2461 cgcgagccgc gagttcggca cgatgacgat gttgttagga agctggcgaa gcgaggtgtt
     2521 gcgccagttg atgtcgatga cctcgccttc gtcgcccgag tcgagcctga tgaagtcgcc
     2581 gagccggacc ttcttggccg cgagcagctg aagcccggcg aacaggttcg cgagcgtgtc
     2641 ctgcagagcg agcgcgatcg ccaggccccc gacgcccagc gccgtcagca tcggcgtgat
     2701 cgagacgccg aacgtctgca gcagcaccag gaacccgacg atgaacacga tcagtcgcgc
     2761 gatgttgatg aagatcgaac tcgacggcag aacgccggat cgccggatcg cgtacagctt
     2821 gagcccgtcg cccacgatgc gcgcgagcac gaacgtcacg ctcct
//
LOCUS       DATLFO010000196         9215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_34961, whole genome shotgun sequence.
ACCESSION   DATLFO010000196 DATLFO010000000
VERSION     DATLFO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34961"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VM841_07985"
     CDS             complement(<1..453)
                     /locus_tag="VM841_07985"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HVL90156.1"
                     /translation="MPGPELARATLAPWFARLLVVVAGAVVLVIETLAARLVAPYVGL
                     TLESHTAAIGVALLGIAAGASIGGRLADGAFPRVVIAAGFGIGGALVFLIRPIVHAIG
                     PGLPPGASSAVIGVALSTLAPVVALSMVPPAVVKMRLGDLHESERADRA"
     gene            complement(532..1770)
                     /locus_tag="VM841_07990"
     CDS             complement(532..1770)
                     /locus_tag="VM841_07990"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL90157.1"
                     /translation="MISKERVSRSIKRARRRRGRTGRNAVAILSVGAMLAGVGAIYLS
                     QGDPVLSDVRQVSVTKSPTTALGGWVNGTNAIGQDNVCATDSGGSNVLDLTGFGFSVP
                     TGSSINGITVSVKGARQSNNGGAMDLVILGGATSPSPTASTGTYPVTTACSSTIFVDN
                     GGATQLFGTTFTPAQVNATTFGVRINSGSLTGGGKLASVDHVTVTIHYTASAPPPVSG
                     TLGISPMVSGETSTKPTFSGTGAQHNGGNGRVRVYLDGSDHPATPHCEAVVAAGGTWS
                     CSNNGVTLSAGSHTVHLRQYTDGIATISGPSASTNFTVAAVPSTPVIVSPADAATVAP
                     NASFSGTCTAGSTVTVTLDGSGIGTTTCDGAGNWVFTPGAPMSDGTHSLVVKATNAVG
                     DSSADSPTRTFNVSSGGPPS"
     gene            2093..3922
                     /locus_tag="VM841_07995"
     CDS             2093..3922
                     /locus_tag="VM841_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HVL90158.1"
                     /translation="MAALAVLTVVVAAGVFSYARIASIRSGLRAAEANLSEARSLAAG
                     DRFEEAAAKVRLAVADADAVRRKTRGPTMRIMSAMPLLGPQARTVRAISEAAAQVSRA
                     ADLLIDAAARSPLLNGVANLGIRGLALDPLLAQIAPVIPTLREASAMLERASQSMSLA
                     PKRGVGAALWDVRRAVETQLAQARTQVDVALHLHEFGTTVANGNPLRILMLSQLTWEV
                     RPAGGFIGSYGILRLDRDGLALEEYDDAVAFPAPDPPYVAPEPIRSALDKPFGLSQTG
                     WWPDFPTAAKAAAEIFRHSGGGEIDGVLAVTQTFLEDIVRGLGEPVTVPGYPDVLTPE
                     NISERTLHHIELKRPLDRPRKKFLIELNDVIFDRLRDVPAERSGAVARALADALRSRH
                     AQIWFADPAHHRPFTLAGWDGRLGPTGTDLLSVIDANMTGSKSNRWVRKKIVYRVYRF
                     GSELRGTVTVRTEHTGPDIPTNHLFNSYLRVYTMNGSAFLPANDREKHSTIVKEDNGL
                     ISLGTWHALRIGESDERTFNWRIGDEAVRDCTYRLLVRPQASASADDYEVRVDLGGET
                     KVFRFKGDAGDQTIEVPIEVPPAGTRLGQLVDGLRRLFRPRSC"
     gene            4176..4604
                     /locus_tag="VM841_08000"
     CDS             4176..4604
                     /locus_tag="VM841_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90159.1"
                     /translation="MGLKRIFGSTLVMAALAAAALVPATSSAHVGPAHMIYGFGTAGA
                     NSVVVNGANQALDSGTAFVGSTVVTLSCVEVIASGPTTHLAAMYGTGGGLRWTILVRQ
                     SAAGDFLVVNNVPTTSAPADANLCGIGNPGATGAGTFWII"
     gene            complement(4733..5704)
                     /locus_tag="VM841_08005"
     CDS             complement(4733..5704)
                     /locus_tag="VM841_08005"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HVL90160.1"
                     /translation="MRIEEAPRVRLAHLPTPVQRAGRLSAALGGAEIWIKRDDCAGGA
                     AGGNKVRKLEFLLGRMLAEEKRVAVTFGGMQSNHARETAAACARLGVACELILVRVVP
                     RSTDAYEQSGNVLLDEVLGAQIHVVDDEQAAAECFMDLYEQHGEAMFGIGPGGSDATG
                     SLGYVQAAFEIAEQMPEPPGRIFVAAGSSGTAAGLLAGLGMTGIDAALHAVAVYHDAE
                     RTREDIERIARELGVATEDLGRLRVHGEWLGEGYGLPTPETVAAIDLFARTEGLVLDP
                     VYTGKAAAAMVAMCRNGGIAAGDRVMFVHTGGLPGLFAYPELFARVP"
     gene            complement(5918..7441)
                     /locus_tag="VM841_08010"
     CDS             complement(5918..7441)
                     /locus_tag="VM841_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90161.1"
                     /translation="MIRRSFCILATLCVAVAGLGLPAAHALPCYSPGLHVFLVTDAKV
                     TIVLDPAFGGLTDTVQLSGPTLVQLSGGGTIGGKEYIDTEILNMDLQGVSTVLGPIQL
                     TESPLLASTGRITQLYSGCFPAESTFDVYVEAHLPMLAHNANGDPVPMRSHINSIPPS
                     GAAYHPPINAPLAIDLYAGASGTTDSDRVGKIIHAMHVPLPEWPFFSVQPNNRSGLDP
                     ASLYYALQAFAPPVPVVPAAMLGLQAFADDVDAISTGIDPMTGGKHEGQAGGEALRFS
                     VAEGSIGAPGSGVESESAPAKIPQEASMDEFEWSSFNNFSNHLFMPGTALGLDTSAPP
                     LPSDDLDALMSDLNSVVKCDGGTPGPAREPIIPVNCMVHPENIPSMFPIYFSLAAGSP
                     TLAAIGATPADILRVDVPFGTPVIDFPAAAMGLGPQDDLDALCNYPNTDANNPFQTAP
                     VFSLRDGSPSLTGDGGDVFAVVAGGGSSLLVRSSFELGLKRPDDLNALKCNLPAYQL"
     gene            7785..8459
                     /locus_tag="VM841_08015"
     CDS             7785..8459
                     /locus_tag="VM841_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90162.1"
                     /translation="MALVYTGAGAQHQSYTGFGGHYTLGLGYMAPPGSPTLPSNATGI
                     EEPVFGLIVCENKDRQSPTGMPGGGAGGQCPSVTVGTGDKVQVFVQDIKNGSGEVSFA
                     ACVDTDYDGNCRPEDPDDYLHFYMCSGLPNPDGRGHSRLSFTHNGPPGPLAFFMMQQV
                     DQTGMCGAVEGYLEGFVLTATGQPPQCSDGRDNDGNGIADWPNDPGCSDPNDDFEWVP
                     PPGYTH"
     gene            complement(8561..>9215)
                     /locus_tag="VM841_08020"
     CDS             complement(8561..>9215)
                     /locus_tag="VM841_08020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HVL90163.1"
                     /translation="QAPAEPKWGGVRPWVLSTGSQVRPAAPPACDSDEYLAAARDVYQ
                     ASLTLSPRQKEIAQYWAGGPGTETPPGMNLRTAMEETHQRGLSTMREARVLAYAGAVI
                     ADAAIAAWDAKFTYWWDRPVTTIRRLWDPNWTSYIGTPPFPGYVSGHSTFSGGTMALL
                     AGFFPDKAVAFKAMATDAAMSRFYGGIHARYDNETGLDMGYAIASLALARAATDGAT"
BASE COUNT         1552 a         3152 c         3050 g         1461 t
ORIGIN      
        1 ggcacgatcg gcgcgctcgg actcgtgcag gtcgccgagc cgcatcttga ccaccgcggg
       61 cgggaccata gacaacgcaa cgacgggagc aagcgtcgac agcgccacgc cgatgacggc
      121 ggaggacgca ccgggcggaa gacccggacc gatggcgtgc acgataggcc ggatcaaaaa
      181 gacgagcgct ccgccgatcc cgaagccggc cgcgatgacc acgcggggga aggccccgtc
      241 ggcgagccgg ccgccgatcg aggcgccggc cgcgatcccg agcagcgcca cgccgatcgc
      301 ggcggtgtgc gactccagcg tcaagccgac gtacggcgcg accagccgcg cggcgagcgt
      361 ctcgatgacg agaaccacgg cgccggcgac gacgaccagc agacgcgcga accagggtgc
      421 cagcgtggcg cgcgcgagtt cgggaccggg catccgctca ggctagcgtg tccgggaacg
      481 caacgacgcc gccccgagat gggacggcgt cgcggatgct tttcggatcg atcagctggg
      541 cgggccgccc gagctgacgt tgaacgtgcg ggtcggcgag tccgcgctgg agtcgcccac
      601 ggcgttcgtg gccttcacca cgaggctgtg cgtcccgtcg gacatcggcg cgccgggcgt
      661 gaacacccag ttgcccgcac catcgcacgt cgtcgtcccg atgccggagc cgtcgagcgt
      721 gacggtgacg gtcgaaccgg ccgtgcaggt gccgctgaac gaggcgttcg gcgcgaccgt
      781 ggccgcgtcc gcggggctca cgatgaccgg tgtggagggc accgccgcga cggtgaagtt
      841 cgtcgaggcg ctcggaccgc tgatcgtggc gatcccgtcg gtgtactgac gaaggtgaac
      901 ggtgtgggag cccgcgctca gcgtgacgcc gttgttcgag cacgaccagg tgccgccggc
      961 ggcgaccacg gcctcacagt gcggtgtcgc cggatgatcg gagccgtcca ggtagacccg
     1021 aacgcggccg ttgccaccgt tgtgctgcgc gcccgtcccc gagaatgtcg gcttcgtgct
     1081 cgtctcgccg ctcaccatcg ggctgatgcc cagcgtgccg ctgacgggcg gcggcgcgga
     1141 tgcggtgtag tggatcgtga ccgtgacatg atcgacgctc gcgagcttgc ccccgccggt
     1201 caagcttccg ctgttgatac gaaccccgaa cgtggtcgcg ttgacctgtg cgggggtgaa
     1261 ggtggttccg aacaactggg tggcgccacc gttatcgacg aagatcgtcg acgagcacgc
     1321 cgtggtaacc gggtacgttc cggtcgatgc cgtcggggac gggctcgtgg cgcctccgag
     1381 gatgaccagg tccatggcgc ccccgttgtt ggattgcctg gcgcccttga ccgagaccgt
     1441 gatgccgttg atcgacgatc ccgtcggaac ggagaacccg aaccccgtca ggtcgagcac
     1501 gttcgatccg ccgctgtcgg tcgcgcagac attgtcctgg ccgatcgcat tcgttccgtt
     1561 gacccagcct ccgagcgcgg tagtcgggct cttggtgacc gagacctgac ggacgtcgct
     1621 caggacgggg tctccttgag agaggtagat cgctccgact ccggccagca tggcgcccac
     1681 cgacagaatc gcgacggcat tgcggccggt ccggccgcgc cggcgacggg cgcgcttgat
     1741 cgagcgcgac acacgttctt tcgagatcac aggttccccc ttaaccccgc cccccggagc
     1801 ggaggacaaa atggttgcaa ctatcctagt tcgaccgggc gaaatcaaac cctgcgggcc
     1861 ttggcttaaa gctgactgaa gttcgcgccg cggcagaatc aggcgagccg gcctcgtatc
     1921 ctgtccgcag ccccgcagcc ggcacccgtg cggttcgtta agacatttcg ttaagacaat
     1981 gatgaccata acccatccgg cgaccggctc tcgacgccgg aactccggct tttcccctct
     2041 tcgccgcatc cgacggcgca ttcgccgttg ggccaagcac caccgcccgg cgatggccgc
     2101 gctcgcggtg ctcacagtcg tggtcgcggc cggggtcttt tcgtacgcgc gcatcgcctc
     2161 gatccggtcg ggccttcgcg ccgccgaggc gaacctatcc gaggcccgat ccctcgcggc
     2221 cggcgaccgg ttcgaggagg ccgccgccaa agtccggctg gccgtggcgg acgccgacgc
     2281 cgtgcggcgc aagacccgcg gacccacgat gcggatcatg tccgcgatgc cgctcctggg
     2341 gccgcaagcc cgaaccgtgc gtgcgatcag cgaggcggcc gcgcaggtct ctcgcgcggc
     2401 cgacctgctc atcgacgccg ccgcccgaag ccctctgctc aacggcgtgg caaacctggg
     2461 catccgcggc ctcgccctcg atccgctgct ggcccagatc gctccggtga tcccgacgct
     2521 tcgtgaggcg agcgccatgc tcgagcgggc gagccagagc atgagcctgg ccccgaagcg
     2581 aggcgtcggt gcggcgttgt gggacgtgcg ccgcgcggtc gagacccagc tcgcacaagc
     2641 tcgcacacag gtggacgtcg cgctgcacct gcacgagttc ggcaccaccg tcgccaacgg
     2701 caacccgctt cgcatcctga tgctctcgca gctgacctgg gaggtccgcc ccgccggcgg
     2761 gttcatcggc tcgtacggga tcctgcgcct cgatcgggac ggccttgcgc tcgaggaata
     2821 cgacgacgcc gtcgcgtttc ccgctcccga cccgccctac gtcgcgcccg agccgatccg
     2881 ctcggcgctc gacaagccgt tcggcctgag ccagaccggc tggtggccgg acttccccac
     2941 cgccgcgaaa gcggccgccg agatcttccg ccactccggc ggcggcgaga tcgacggcgt
     3001 gctcgcggtg acgcagacgt tcctcgagga catcgtgcgt ggcctcggcg agccggtcac
     3061 cgtgcccggc taccccgacg tgctcacacc cgagaacatc agcgagcgga ccctgcatca
     3121 catcgagctc aaacggccgc tcgaccggcc gcgaaagaag ttcctgatcg agctcaacga
     3181 tgtgatcttc gatcgcctgc gggatgtgcc ggcggagcgg tccggtgcgg tcgcgcgcgc
     3241 gctcgccgac gcgctgcgca gccggcacgc gcagatctgg ttcgccgatc ccgcgcacca
     3301 ccgtccgttc acgctcgcgg gctgggacgg ccggctcggt ccgaccggca ccgacctgct
     3361 gtcggtgatc gacgcgaaca tgaccggcag caagtccaac cggtgggttc gcaaaaagat
     3421 cgtctatcgc gtctaccgct tcggcagcga gctgcgcggc accgtcacgg ttcgcaccga
     3481 gcacaccggc ccggacatcc cgaccaacca tcttttcaac agctacctgc gcgtctacac
     3541 catgaacggg tcggccttcc tgccggcaaa cgaccgcgag aagcactcga ccatcgtcaa
     3601 ggaagacaac ggcctcatct cgctcgggac gtggcacgcg ctccggatcg gcgagagcga
     3661 cgagcgaacc ttcaactggc gaatcggcga tgaggccgtg cgcgactgca cgtatcgtct
     3721 gctcgtgcgt ccgcaggcga gcgcgtccgc cgacgactac gaggttcgcg tggatctcgg
     3781 cggcgagacg aaggtgtttc gcttcaaagg tgacgcgggt gaccagacga tcgaggtgcc
     3841 catcgaggtg ccgcccgccg gcacccgcct cgggcagctg gtcgacggcc tgcgccgcct
     3901 gttccgcccc cgctcctgct aacgacgggc gactgcctta cacgcgcctc tcgagcgccc
     3961 tcgcgccaaa ggcacgatcg gctgatcagc gggcacctcg tagggcgtac ggcagagggg
     4021 ctcctcgagc gacgtcggtg ccgagcgccg gtccggcggg ggcatgcaga aatacacaga
     4081 tcgagcggtc tccggacacg gaccggcacc ccccaggtgc gagacacttc ccgcagccgc
     4141 cgggcgcagg gcccggcacg atggggaggc tacagatggg actcaagcga atcttcggca
     4201 gcacgctggt gatggccgcg ctcgcggcgg ccgctttggt tccggcgacg tcgtcggcgc
     4261 acgtcgggcc ggcgcacatg atctacggct tcggcaccgc cggcgcgaac agcgtggtcg
     4321 tcaacggagc caaccaggca ctcgactccg gcacggcctt cgtgggctcg accgttgtga
     4381 cactgtcgtg cgtcgaggtg atcgcctccg gaccgaccac gcacctggcc gccatgtacg
     4441 gcaccggcgg cggcctccga tggacgatcc ttgtccggca gtcggcggcc ggcgacttct
     4501 tggtcgtcaa caacgttccc acgacgtcgg ctccggcgga cgcgaatctg tgcggcatcg
     4561 gcaacccggg cgcgaccgga gccggcacgt tctggatcat ctaagcggaa cgggtttctc
     4621 gaatgcgcgg cggcttcggc cgccgcgcat tatgcgtctt tgttgccggc ggtgctcgag
     4681 actcgctggc accgcgcgcg acaatcaatc gagtcgtcgg ggattcccta gtctagggga
     4741 cccgggcgaa caactccggg tacgcgaaca gcccgggcag gccaccggtg tgcacgaaca
     4801 tgacacgatc gccggccgcg atcccgccgt tccggcacat cgcgaccatg gccgcggccg
     4861 cctttccggt atagacggga tcgagcacga gcccctcggt gcgagcgaac agatcgatgg
     4921 ccgcgaccgt ttcgggcgtc gggagaccgt agccttcgcc gagccactcg ccgtgcacgc
     4981 gaagccggcc caggtcctcg gtcgccacgc cgagctcgcg cgcgatgcgc tcgatgtcct
     5041 cgcgagtgcg ttcggcgtcg tggtagacgg cgaccgcgtg cagggccgcg tcgatccccg
     5101 tcatgcccag gccggcgagc agaccggccg ccgtcccgct cgagccggcc gcgacgaaga
     5161 tccggcccgg cggctcgggc atctgctcgg cgatttcgaa ggcggcctgc acgtacccga
     5221 gcgatccggt cgcgtccgag ccgcccggac cgatgccgaa catcgcttcg ccgtgctgct
     5281 cgtagaggtc catgaagcac tccgccgctg cctgctcgtc gtccaccacg tgaatctgcg
     5341 cgccgagcac ctcgtcgagc aagacattgc cgctctgctc gtacgcatcc gtgctgcgcg
     5401 gcacgacgcg cacgaggatc agctcgcacg cgaccccgag ccgcgcgcat gcggcagcgg
     5461 tctcgcgcgc gtgattcgat tgcatgccgc cgaatgtgac ggcgacgcgc ttttcttccg
     5521 cgagcatgcg gccgagaagg aactcgagct ttcggacttt gttgcctccg gccgctccgc
     5581 cggcgcagtc gtcgcgcttg atccagatct ccgcgccgcc cagcgcggcc gagagccggc
     5641 ctgcccgttg gacgggcgtc ggcagatgag cgagccgaac ccgcggggcc tcctcgatgc
     5701 gcatgctcag aacctagcag ctcgatttgc cttcgatcct ccgacatgca ccatttccgc
     5761 tgcgggccat gactgcttgt ttccccgcgc gccgctccga gcaaaacgac gtcttcgcaa
     5821 cgcacatcat gatggcgggg tcgatcggta tggagcgcac agatcacgaa agcggcctgc
     5881 tcgattgtcc gagcaggccg cttcttatca agtgaaatca gagttgatac gcaggaaggt
     5941 tgcacttcaa agcgttgagg tcgtccggcc ttttgaggcc gagctcgaag gaggagcgaa
     6001 ccagcagcga agaaccgccg ccggcgacca ccgcgaacac atcgcctccg tcgcccgtca
     6061 acgacggtga gccgtcgcgc aacgaaaaca ccggtgcggt ttggaacggg ttgttggcat
     6121 cggtgttcgg atagttgcac agggcatcga gatcgtcctg cggcccgaga cccatcgcgg
     6181 ctgccggaaa atcgatcacc ggcgtgccga acggcacatc cacccgcaag atgtctgccg
     6241 gcgtggcccc gattgctgca agcgtcggcg aaccggctgc gagagagaag tagatcggga
     6301 acatgctcgg aatgttttcc ggatgcacca tgcagttcac cgggatgatc ggctcccggg
     6361 cagggccagg cgtgcctccg tcgcacttca cgaccgagtt caagtccgac atcagcgcgt
     6421 ctaagtcgtc actgggcaac gggggcgccg acgtgtccag accgagcgcc gtacccggca
     6481 tgaacagatg gttgctgaag ttgttgaagc tcgaccactc gaactcgtcc atggatgctt
     6541 cttgcgggat ctttgccggc gcgctttcac tttcgacacc cgaaccgggg gcgccaatcg
     6601 acccctcagc gaccgagaac cgcaatgcct ctccgccggc ctgtccctcg tgctttccgc
     6661 cggtcatcgg atcgattccg gttgagatcg cgtccacatc atcggcaaaa gcttgaagcc
     6721 cgagcatggc cgccggcacc accgggaccg gcggggcgaa tgcctggagt gcgtaataga
     6781 gcgatgcggg atcgagtccc gaacggttat tcggctgcac cgaaaagaac ggccattcgg
     6841 gaagcggaac gtgcatcgcg tgaatgattt tgccgacgcg atcactgtcg gtcgttcctg
     6901 atgctcccgc atacaggtca atcgccaacg gagcgttgat gggcgggtga tacgccgcgc
     6961 ccgacggcgg gatcgagttg atgtgcgagc gcatcggaac cgggtcgccg ttggcgttgt
     7021 gcgcgagcat cggcaggtgc gcctcgacat agacatcgaa cgtgctctcg gccgggaaac
     7081 agcccgaata gagctgcgtg atgcgacccg tggatgcgag caacgggctt tccgtcagtt
     7141 gaatcggtcc gagcacggtg ctgacgcctt gcagatccat gttgaggatc tcggtatcga
     7201 tgtactcctt cccaccgatg gtcccgccgc cggatagttg cacgagcgtg ggaccactga
     7261 gttggaccgt gtccgtgagc ccgccgaagg cggggtcgag cacgatcgtc acctttgcgt
     7321 cggtgacgag gaagacgtgg agtcccggcg aatagcacgg aagggcgtga gccgccggaa
     7381 gccccaggcc cgcgacggca acgcaaagcg tcgcgagaat gcaaaacgaa cgtctgatca
     7441 cgtggatcac tcccccttgg gatccttctc ccgtggtccc ggaccgccgg ccacctccct
     7501 cgcccccctg ccacagccgc cggttttctc ttttcgttgg attcgtcacc aatctcccat
     7561 tccctgcttg cttgccggaa gcggttttat gcgtcgaccg cggaatcccg cgcacaggcc
     7621 cgataagggc tcatttttca caaagagaag gaatcgggac aagcgccgcc gaaccaagcc
     7681 ttagggggaa tcagaaatcg ccccatcggt ccggcgtagg gacgccggag aaagggggtc
     7741 cgatgaggac tcgtttcgcg gttgccgcgg ttgcggcagt agcaatggcg cttgtgtaca
     7801 ccggtgccgg cgcgcaacac caaagctaca ccggcttcgg aggccactac acgctcgggc
     7861 tcgggtatat ggctcctccc gggtcgccga cgctgccgtc gaatgccacc ggcatcgagg
     7921 agcccgtgtt cgggctgatc gtgtgtgaga acaaagatcg ccaaagcccc accggaatgc
     7981 ccggaggcgg cgcaggcggc cagtgcccgt cggtgaccgt cggcacgggc gacaaggtcc
     8041 aggtgttcgt gcaggacatc aagaacggat ccggtgaagt ttcgtttgcg gcctgcgtcg
     8101 ataccgacta cgacggcaac tgccgccccg aagacccgga cgactacctg cacttctaca
     8161 tgtgcagcgg tctgccgaat ccggatggtc gtggccactc acggttgtcg ttcacgcaca
     8221 acgggccgcc gggaccgctc gcgttcttca tgatgcagca ggtggaccag accggcatgt
     8281 gcggcgccgt tgagggctac ctcgagggct tcgtcctgac ggcgaccgga cagccgccgc
     8341 aatgctcgga cggtcgtgac aacgatggca acggcatagc cgactggccg aacgacccgg
     8401 gctgcagcga ccccaacgat gatttcgagt gggtgccgcc gccgggatac acgcactagc
     8461 gaacacacgt gatcgttacg gcccgggcat gacatgcccg ggccgtaacg cttcccatca
     8521 gacgcgggta ttcggacagt cgatccgtcg gacggccccg ctacgttgcg ccgtccgtgg
     8581 ccgcgcgcgc gagggcgagc gatgcgatcg catagcccat gtcgagcccc gtctcgttgt
     8641 cgtagcgcgc gtggatgccg ccgtagaacc gtgacatggc ggcgtccgtc gccattgcct
     8701 tgaacgcaac ggccttgtcg gggaagaacc ccgccagcag cgccatcgtc ccgcccgaga
     8761 acgtcgagtg acccgatacg tagcccggga acggaggcgt gccgatgtac gacgtccagt
     8821 tcggatccca cagccggcga atcgtggtca cgggccggtc ccaccagtag gtgaactttg
     8881 cgtcccaagc ggcgatcgcg gcatcggcga tcaccgcacc cgcataggca agcacccgag
     8941 cctcgcgcat cgtagacaac ccgcgctgat gcgtctcttc catcgccgtc cgcaaattca
     9001 tgccgggcgg cgtttcggtg ccgggcccgc ccgcccagta ctgggcgatc tctttctgtc
     9061 gcgggctcag cgtgagcgaa gcctgataca cgtcgcgcgc agcggcgagg tattcgtcag
     9121 agtcgcaagc aggcggcgcg gccggacgca cctgactccc ggtgctcagc acccacggcc
     9181 ggacgccacc ccacttgggc tcggccggcg cctgc
//
LOCUS       DATLFO010000197        12858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_63439, whole genome shotgun sequence.
ACCESSION   DATLFO010000197 DATLFO010000000
VERSION     DATLFO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63439"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1158
                     /locus_tag="VM841_08025"
     CDS             367..1158
                     /locus_tag="VM841_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90164.1"
                     /translation="MSSLSRNNVRVFGNPAGKPMIFAHGFGCDQNMWRYVTPAFEDEY
                     RVVLFDHVGSGGSDLAAYDFVKYSSLQGYADDVVEICRELELVDVVFVGHSVSSMIGA
                     LAATAAPDLFESLVMVGPSPRYINDDGYVGGFGREDIDDLLVSLESNYLGWSSQMAPV
                     IMGNGDRPELGAELTESFCRTDPEIAAHFARVTFLSDNRADLAGVKTRTLVLQCSSDV
                     IAPEEVGRYVTENLPAAELVVMKATGHCPNLSAPEETIAAIKAFL"
     gene            1164..2360
                     /locus_tag="VM841_08030"
     CDS             1164..2360
                     /locus_tag="VM841_08030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVL90165.1"
                     /translation="MAGGDERIVADPVVLDVIEESAEDLYENAPCGYLSTLGDGTIVK
                     VNETFLRWTGHRREDLVRRKRFHELLTAGGRIYYETHYAPLLRMQGTVREIAVDLVCS
                     DGRRLPALINAMLIRDDRGEPLAIRTTILDATERRGYEQELLRERKKARESETEARLL
                     ARTLQESLIPPEPARIPGLEVAAAYRPAGSGEAIGGDFYDVFETANNNWVAVIGDVSG
                     KGADAARVTAAARYTLRAAAMRSTKPRLVLSALNDALLHHGFGRFCTVAYSHIAFRDG
                     TVRVTVACAGHPLPVIVSPDATIRTEGRPGTMVGAFEEVVLEDTTFELKPGEAMILYT
                     DGIPEGRRRDGAFYREDRLHAVVAANAVRSAAELCAAITDDVVSFQGGHPRDDIALLV
                     VRAPGR"
     gene            2384..2713
                     /locus_tag="VM841_08035"
     CDS             2384..2713
                     /locus_tag="VM841_08035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90166.1"
                     /translation="MSDPVVRNQPERERFVILVEDELAGWSDYESVPGLRSFMHTAVN
                     DRFQGRGLAGELIRAALDATRSEGLLVEPYCPYVRGFIEKHPEYLDLVPADRLDEFGL
                     SAAAPGS"
     gene            complement(2821..3447)
                     /locus_tag="VM841_08040"
     CDS             complement(2821..3447)
                     /locus_tag="VM841_08040"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HVL90167.1"
                     /translation="MTHEIRHPDPPTMSRTFAAVTQVLDDFAVPYLVIGSIAADVCGS
                     EGPWPREGSDIDLFLTESDARRAHAILGENGFHIEDTDEDWLLKAYREGVLIDLIFRA
                     ASTITLDEEMLERARTKTVEGRRVRIIAPEDYIVIQSRTFSRETPSHWFSACAVLEKS
                     SIDWDYLLRRAEDRRDRAMALLMFARSNGITVPGNVLDRLYEPALARH"
     gene            3493..4230
                     /locus_tag="VM841_08045"
     CDS             3493..4230
                     /locus_tag="VM841_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HVL90168.1"
                     /translation="MNLACLVLAAGLGTRLRPLTDLRPKPLCPVNNVPLVDANLARGA
                     RHAESVAVNVHHHRDRMVAHLEGRAHLSVEPEPLGTAGALGNLREWIDGRDVLVINAD
                     AWHADSLDDLVRGWDRERPRLLVVEDRSRPDFHGRYRYTGACVLPARVAFRLPSEFSG
                     LYAEVFAREPIDLCPSAEPFFDCGTIRDYHAANLHASGGRTVAGEGARILGRAERCVL
                     WPGVTVGPDEYLTDAIRAADDVTVSAR"
     gene            4586..5461
                     /locus_tag="VM841_08050"
     CDS             4586..5461
                     /locus_tag="VM841_08050"
                     /inference="COORDINATES: protein motif:HMM:NF017808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90169.1"
                     /translation="MGARRPVVVTVLAVAALFVSATPAYARTDNRSKPVVYVHGWSSS
                     SATDCNMWNNMDNQLRAWGLTGTKVTVKYYSADTNCTDSLLSYGSHTAHYGSSHTSHN
                     NGTDIRHLSYHLAQMINAKFGANPVDIVAHSMGGLITRYMVGEVARRNADFPATLNVE
                     DIVTMGTPHNGTGWANWCGGTQCVQMRPGSSFINYVNANAQNPKAGDTDWTLMGSDSD
                     GIVSASSATSMSANHKVIYLSTNSIGHSDFYNLTTDARTADVDYNDYGGTWYRWYDAP
                     WPVRWSDFALYVGTW"
     gene            complement(5513..6370)
                     /locus_tag="VM841_08055"
     CDS             complement(5513..6370)
                     /locus_tag="VM841_08055"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL90170.1"
                     /translation="MILVTGATGNVGSAVVAELSARGIPCRAFVRDPGQAAERLGPDV
                     ALAVGDFNDAASLARAMEGADAIFLSSADHPGKVGHETAVIDVAVAAGVRKIVKASTV
                     AAEIGSPRPPFDWNARIEKHLRESAVESVVLQSYFYMTNLLASAETVAKRGMLIAPLG
                     GAKIAMIDPRDTGATGAVALTTGDYDGSTLTLSGPEAVTYDEVAKELASAIGGTVEFV
                     PVPDEAARQSFEQMGLPAWLVTHFAHLFPFLRDGAQAQPTDTVRAVTGREPRGVATWV
                     RDHAALFRA"
     gene            complement(6384..7238)
                     /locus_tag="VM841_08060"
     CDS             complement(6384..7238)
                     /locus_tag="VM841_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL90171.1"
                     /translation="MGSGSAIANVDQARGWDGPEGDNWTDNEHWYNDAVRSLAPHLSR
                     AAAIRASDQVLDIGCGCGQSTREAARAAREGSALGIDLSSRMIARARQRATEEGLANV
                     CFEHGDAQVFSFEPASVDIAVSRFGCMFFSDPVAAFGNVARALRPGGRIALMVWREMG
                     RNEWITSVRDALAAGRDLPEPPPDAPSPVSLADPDRVKRILTEAGFEELDLESVDEPM
                     RFGADAERGFEGATRLSIVTGLLADLDDATRRDALHRLRQALAGRQTPDGVLFPASAW
                     LVRAAKSR"
     gene            7289..7927
                     /locus_tag="VM841_08065"
     CDS             7289..7927
                     /locus_tag="VM841_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVL90172.1"
                     /translation="MPSRGNSRPPAGLFGVLGLLAVRPMSTYELATHFDRSLGRMWPR
                     TRSKLFEMPKRLVEMGFARAAKGATGRRAKTVYTITPKGRRALAAWLAEPGGGPVIEF
                     EALMKVFYGDQGGRDAMIANLEAAKDWAREQIDEHIEVARTYLEGRGAFQDRAAINQI
                     TGRFHVELALTVHRWATRSLATVRTWPADPREAEMSRAALEDVLRELEVARE"
     gene            complement(7969..8184)
                     /locus_tag="VM841_08070"
     CDS             complement(7969..8184)
                     /locus_tag="VM841_08070"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HVL90173.1"
                     /translation="MPGIGIGVVLIALGAILRFAVTADAEGFNIGTIGVILMIVGGVA
                     LLLSALFWSSWFGTRETRVSDGHAHGH"
     gene            8379..9854
                     /locus_tag="VM841_08075"
     CDS             8379..9854
                     /locus_tag="VM841_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90174.1"
                     /translation="MLKRLFVSAVAVAMLVPFGGAQAAPIDTWPVTHGIDCLNDPFNP
                     TRSDGTKDPAGDPLPGTAAWIERDRQRLECDNQRDHDRRYHPHNSLNSGRYGEDWYRN
                     PHRFDGRRWRYDYIAADAQGLYGIPGVPAAEIFRPCKPLTCLNKPAELERFAPPYPVV
                     VIHHGFIAQMTHHRFNAQVYAEAGYMAIVVNGIHPVTGVPNVQDSANGAQVLDWLASP
                     ASGEIGRQADLSRVAMVGHSQGAAAALSNQGDPRVDAIVAWDGGDSISDLNCKDGNPC
                     APVMYQRTDGAFSAPQATARAGYPATRERGVPTYLEHKARDMNVYHITMRASNHIDWN
                     GNGVGSLAGSRLAEPWINYYTLAWLDLHLRGQLVRDGNGNIVTQHGRSAWDERWFRQG
                     IVNRAFNMLTSKRFLGGTIDKHNISQGFYDPVQMATSGDPLFGGNVPYQIEDLWVVDR
                     LSPEFRNLCSLTVPNYVTGGTLATADTGVAGDIRLTGCPVL"
     gene            complement(10211..10957)
                     /locus_tag="VM841_08080"
     CDS             complement(10211..10957)
                     /locus_tag="VM841_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HVL90175.1"
                     /translation="MTPSERNDAPKDRPRDFSDLRAVFISCTLKKSPQVSHTEGLARI
                     AVEIMQRNGVDVEMIRSVDHVIPPGVYPDMTEHGWDRDDWPGIFAKVQAADILVLCSP
                     IWLGEKSSECTKVIERLYGQSGELNDKGQYLYYGRVGGCLITGNEDGVKHCSMNILYS
                     LQHLGYAIPPQADAGWIGEAGPGPSYLDPGSGGPENDFANRNTSFMSWNLMHLARMIK
                     DAGGIPAHGNQRSEWDAGCRPDFPNPEHRR"
     gene            11306..>12858
                     /locus_tag="VM841_08085"
     CDS             11306..>12858
                     /locus_tag="VM841_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90176.1"
                     /translation="MRSTRKIALLAVAVLAALTVRVAPADPVKGLVEVTGTVIPNQYI
                     VVFHDTLVSDVGVAASTMARENDARIQFVYQHALKGFSAEMSPAAAAKMALDRRVAWI
                     EPDQEMELIATQSNPPWGLDRIDQRDRPLNQSYVYNATGSGVKSYIIDTGIRRTHADF
                     GGRAIVGYDAITSGGSANDCNGHGTHVAGSAGGTTYGVAKSTTLVAVRVLDCNGSGSN
                     SGVIAGVDWVTADHLAGQPAVANMSLGGGASSALDTAVNNSINDGVTYVVAAGNGDFF
                     GNPQNACNYSPARVAAAITVGATDSNDARASFSNFGTCLDIFAPGVSILSAWHSSDTA
                     TNTISGTSMASPHVAGAAAMYLQGNTSASPATVSNALTSNASSGKVSNPGTGSPNLLL
                     YTAFIGGGGGNQAPTANFTYSCSGLTCNFTDTSTDSDGTISSRSWNFGDGTTSTATNP
                     SKTYSAAGTYTVTLTVTDNGGATGSTSKSVTVSSTSSPCGADPNTSIPNLTNGVFTSG
                     TAASTTVRW"
BASE COUNT         2141 a         4371 c         4209 g         2137 t
ORIGIN      
        1 cagttcctgc ttcatcttcg cgagcaggtc ttcccgggtc ttgacgcccc gttcggtcgt
       61 ggcgtcgatg tcctccatga tcttcttcat ggtcttgtgg tcttgtttca acagtgagat
      121 ggcatccatg aggcgtcctc ccgggtccgt tgtcgtcgga gatcgaatac ccaatcgagc
      181 aagcagtcac acggaaggtt cgcggtactc gacccgcggt agccgcccgg gcaggttgct
      241 cgccggggaa gcagatgtgt gtgctctcca aggattgagc ggctttgccg gacacagcgg
      301 ggcgttcggt gcgctcgggg cgtttcacgg cgacgacggt ctatgagggc ttctatactc
      361 gctgcaatga gctcactttc ccggaacaac gtgcgcgtct tcggaaatcc cgccggcaag
      421 cccatgatct tcgcccacgg gttcggctgc gaccagaaca tgtggcgtta cgtcaccccc
      481 gctttcgagg acgagtaccg cgtcgtcctg ttcgatcacg tcggctcggg cggctccgat
      541 ctggccgcct acgatttcgt caagtattcg tcgctgcagg gctatgccga cgacgtcgtc
      601 gagatctgcc gcgagctcga gctcgtcgat gtggtcttcg tcggacactc ggtgtcgtcg
      661 atgatcggtg cgcttgccgc gacggccgct ccggacctgt tcgaaagtct cgtgatggtc
      721 gggccgtccc cgcgctacat caacgacgac ggatacgtcg gcggattcgg ccgcgaggac
      781 atcgacgatc ttctggtctc gctcgagagt aactatctcg gatggtcgtc gcagatggcg
      841 ccggtgatca tgggcaacgg cgatcgcccc gagctgggtg ccgagctgac ggagagcttc
      901 tgccgcaccg atcccgagat cgccgcccac ttcgcccggg tgacgttcct gtcggacaat
      961 cgcgcagacc ttgccggggt caagacgcga accctggtgc tgcagtgctc ctccgacgtc
     1021 atcgcaccgg aggaggtcgg tcggtacgtg accgagaacc tgccggcggc cgagctggtg
     1081 gtcatgaagg cgaccggtca ttgtcccaac ctcagcgcgc ccgaagagac catcgccgcg
     1141 atcaaggcgt tcctataaag ccgatggccg gcggggatga gcggatcgtg gccgacccgg
     1201 tcgttctcga cgtgatcgag gagagcgccg aggatctcta cgagaacgct ccctgcgggt
     1261 atttgtccac cctcggcgac ggcaccatcg tcaaggtcaa cgaaaccttc ctgcgttgga
     1321 ccgggcaccg gcgcgaggac ctcgtccggc gcaagcgatt ccacgagctg ctgaccgccg
     1381 gtgggcggat ctactacgaa acccattacg cgccgctcct ccgcatgcag ggaaccgtgc
     1441 gcgagatcgc ggtggacctc gtctgctcgg acggccgccg gctccctgcg ttgatcaacg
     1501 cgatgctgat ccgcgacgac cggggagagc cgctggccat ccgcacgacg atcttggacg
     1561 cgaccgagcg gcgaggctac gagcaagagc tgctacgcga gcgaaagaag gcacgcgagt
     1621 cggagacgga ggcgaggctt ctcgcgcgga cgctccaaga gagcctgatc ccgccggagc
     1681 cggcacgcat acccggcctc gaggtcgcgg ccgcctaccg gcccgccggc agcggcgagg
     1741 ccatcggcgg cgacttctac gacgtgttcg agaccgccaa caacaactgg gttgccgtca
     1801 tcggcgacgt gagcggaaaa ggcgcggatg cggcgcgggt cacggcagcg gcccgctata
     1861 cgctccgcgc cgcggccatg cgcagcacca agcctcgtct cgtgctctcc gccttgaacg
     1921 acgctctact ccatcacggg ttcggccggt tctgcaccgt cgcgtattcg cacatcgcgt
     1981 tccgcgacgg caccgtccgc gtcaccgtcg cgtgcgcggg tcatccgctt ccggtcatcg
     2041 tctcgcccga cgcaaccatc cgcaccgaag gccggccggg cacgatggtc ggcgcgttcg
     2101 aagaggttgt cctcgaggac acgaccttcg agctcaagcc cggcgaggcg atgattctct
     2161 acaccgacgg cattcccgag ggccgtcgcc gcgacggagc gttctaccgc gaggatcggc
     2221 tccacgcggt cgtcgccgca aacgccgtcc ggtccgcagc cgaactgtgc gctgccatca
     2281 ccgacgacgt cgtgtcgttc cagggcgggc atcctcgcga tgacatcgcc ctgctcgtcg
     2341 tgcgagcgcc cggccggtag gagttcgcgt tagcatggcg cccatgtccg atcccgtcgt
     2401 gcggaaccag cccgagcgcg agcgtttcgt gatcctggtc gaggacgaac tcgccgggtg
     2461 gagcgactac gagtcggtgc ccggcctgcg atcgttcatg cacaccgccg tcaacgaccg
     2521 gttccaaggc cggggacttg ccggggaact gattcgcgcg gcgctcgacg caacacgctc
     2581 cgagggcctg ctcgttgagc cctactgccc ctacgtccga gggttcatcg agaagcaccc
     2641 cgagtatctc gacctggtcc cggccgaccg cctcgacgag ttcggtcttt cggccgccgc
     2701 gccgggatcc tgacggcgcc ggctaccgca cgcggaacga gagatcgatc gacgtgccgt
     2761 cgtcgagcgc gatgcggagg cggtgtcggc ccggcgagcg gcgcgtgcgc ggagccgcgt
     2821 ttagtggcgg gcgagggccg gctcgtagag ccggtcgagc acgttgccgg gcaccgtgat
     2881 gccgttgctg cgcgcgaaca tcagcagcgc catcgcccgg tcccggcggt cttccgctcg
     2941 gcgaagcagg tagtcccaat caatcgagga cttctccaga acggcgcagg cgctgaacca
     3001 atgcgacgga gtctcgcggc tgaacgtgcg ggactggatc acgatgtagt cctcgggggc
     3061 gatgatgcgg acgcgccggc cttcgaccgt cttggttctg gcgcgttcga gcatctcctc
     3121 gtccagggtg atcgtgctcg ccgcacggaa gatcaagtcg atcaacaccc cttcccggta
     3181 ggccttgagc aaccagtcct cgtcggtgtc ctcgatgtgg aagccgttct cgccaaggat
     3241 cgcgtgagcg cggcgagcgt cgctctcggt caggaacagg tcgatgtcgg agccttcccg
     3301 gggccacggc ccttcgctgc cgcagacgtc ggcagcgatg ctgccgatca ccagataggg
     3361 aacggcgaag tcgtcgagca cctgcgtgac ggccgcgaag gtccgcgaca tcgtcggcgg
     3421 gtcgggatgt ctgatctcgt gcgtcatcgc tggaaggctt cccctgctca gggtcgtggt
     3481 aaaccgctcc ctgtgaatct cgcgtgcctc gtcttggccg ccggcctcgg gacgagactg
     3541 cggccgctga ccgacctgcg gcccaagccg ctgtgtccgg tgaacaacgt tccgctcgtg
     3601 gatgcgaacc tcgcccgggg ggcccggcat gccgaatcgg tggccgtcaa cgtccatcat
     3661 catcgggatc gcatggtggc ccatctcgag ggccgcgctc acctgtcggt ggaaccggag
     3721 ccgctcggga ctgcgggcgc gctcgggaat cttcgggagt ggatcgacgg ccgtgacgtg
     3781 ctcgtgatca acgccgacgc atggcatgcg gattccctcg acgatctggt ccgcggatgg
     3841 gatcgtgagc ggcctcgcct gctggtcgtc gaggaccggt cgcgacccga cttccatggc
     3901 cggtatcggt acacgggtgc gtgcgtgctc cccgcgcggg tcgccttccg gctgccctcc
     3961 gagttctccg gcctgtacgc cgaggtcttc gcccgcgagc cgatcgatct gtgtccctcc
     4021 gccgagccgt tcttcgactg cggaaccatc cgcgactatc acgccgcgaa cttgcacgcc
     4081 tcgggcgggc gcaccgttgc cggggaaggc gcgcggatcc tcgggcgcgc ggaacgctgc
     4141 gtgctgtggc cgggtgttac ggtcggtccc gatgagtatc tcaccgacgc gatccgcgcc
     4201 gccgacgacg tgaccgtctc ggcacgctag tcgcacaggt tcccggccca ccgccgactt
     4261 cttcatcgag aactacggac ccacgctcaa ggcattcgaa gccgccggcg agaaacgcga
     4321 cgcgcttcgt gcggatttga tcgcgctcgc cgaggccaac aatcgcagcg ccaccggaac
     4381 cttcgtcagc gatcgggagt acgtggcggt cgtcgcgaag aaagcctgat cgcatcgttg
     4441 cgggccggcg gccgccaaga ccgtcggccc gcgatccgcc tccgggggcc gtgcagagca
     4501 agcgaccttg acaaccaagc cccacctcat tacaaagcgg aaattccggt gaaatccatc
     4561 ggatcggttc aacaggggag ggagcatggg tgcacgacgt cccgtcgtcg ttacggtctt
     4621 ggccgtcgcg gccttgttcg tgtcggcaac gcccgcgtac gcacggaccg acaaccggtc
     4681 caagccggtc gtctatgtcc acggctggag ctcgtcgtcg gccacggact gcaacatgtg
     4741 gaacaacatg gacaaccagt tgcgcgcgtg gggtctaacc ggcaccaagg tgacggtcaa
     4801 gtactactcg gccgacacca actgcaccga ctcgctgctc agctacggtt cgcacaccgc
     4861 gcactacggc agctcgcaca ccagccacaa caacggcacg gacatccggc atctgtccta
     4921 ccacctggcc cagatgatca acgccaagtt cggcgccaac ccggtggaca tcgtcgcgca
     4981 ctcgatgggc gggctgatca cgcgctacat ggtcggtgag gtcgcccgac gaaacgccga
     5041 cttcccggcg acgctcaacg tcgaagacat cgtgaccatg ggaacgccgc acaacggaac
     5101 cggctgggcc aactggtgcg gcgggacgca gtgcgtgcag atgcgacccg gctcgtcctt
     5161 catcaactac gtcaacgcca acgcgcagaa cccgaaggcc ggcgacaccg actggacgct
     5221 gatgggctcc gacagcgacg gcatcgtcag cgcgtcgtcg gccacgtcga tgagcgcgaa
     5281 tcacaaggtg atctacctga gcacgaatag catcggtcac agcgacttct acaacctcac
     5341 gaccgacgcg cgaacggccg acgtggacta caacgactac ggcggcacct ggtaccgctg
     5401 gtacgacgcc ccgtggccgg tccgttggag cgatttcgct ttgtacgtcg gcacctggta
     5461 agcgaacgac gagaagggcc cgccgctggt cggcgggccc ttttgcatgc gtctaggcgc
     5521 gaaacagcgc cgcgtggtcg cggacccacg ttgcgacgcc gcggggctcg cggccggtga
     5581 ccgctcggac ggtgtcggtg ggttgcgcct gggccccgtc gcgcaggaac gggaacagat
     5641 gggcgaagtg cgtgacaagc caggccggca ggcccatctg ctcgaaggac tgccgcgcgg
     5701 cctcgtcggg gaccgggacg aactccacgg tcccgccgat cgcagaggcg agctctttgg
     5761 caacctcatc gtaggttacg gcctcggggc cgctaagcgt gagcgtgctg ccgtcgtagt
     5821 cgccggtcgt cagcgcgacg gcgccggtcg cgcccgtatc ccgcgggtcg atcatcgcga
     5881 tcttggctcc gcccagcggt gcgatcagca tgcctctctt cgcgaccgtc tcggccgagg
     5941 cgagcagatt ggtcatgtag aagtaggact gcagcacgac cgattcgacg gcggactcgc
     6001 gcagatgctt ctcgatgcgt gcgttccagt cgaacggcgg ccgcggcgag ccgatctcgg
     6061 cggcgaccgt cgaggccttc acgatcttgc gcactcccgc cgcgacggcc acgtcgatca
     6121 cggccgtctc gtgacccact ttgccgggat gatcggccga ggacaggaat atcgcgtcgg
     6181 ctccctccat cgcgcgtgcg agcgaggccg cgtcgttgaa gtctccaacg gcaagcgcga
     6241 cgtccggccc gagccgctcc gcggcctgtc cgggatcgcg cacgaacgcg cggcacggga
     6301 ttccgcgcgc cgacagctcg gcgacgacgg cgcttccgac gttgccggtc gctccggtta
     6361 ccaggatcat gatcatccac tcctcagcga gatttcgcag cgcgcaccag ccacgcgctc
     6421 gcagggaaca gcactccgtc cggggtctgg cgaccggcga gcgcctgccg gaggcggtgc
     6481 agcgcgtcgc ggcgggtcgc atcgtcgagg tcggcgagca gcccggtcac gatgctcaaa
     6541 cgcgtggcgc cttcgaagcc gcgttcggcg tcggcgccga accgcatcgg ctcgtcgacg
     6601 gactcgagat cgagctcctc gaatccggcc tcggtcagga tgcgtttgac gcgatccgga
     6661 tcggcaagcg agaccggcga tggcgcgtcc ggtggcggtt cgggcagatc gcgtccggcg
     6721 gccagcgcgt cgcgcacgct cgtgatccat tcgttgcggc ccatctcgcg ccagaccatc
     6781 aacgcaatcc gcccacccgg ccgcagagcc cgcgcgacgt tgccgaacgc ggccaccgga
     6841 tccgagaaga acatgcaccc gaaccggctg acggcgatgt cgaccgaggc cggctcgaac
     6901 gaaaacactt gagcgtcgcc gtgctcgaag cagacgttcg cgagtccctc ctcggtcgcg
     6961 cgctgccgtg cacgcgcgat catgcgtgac gacaggtcga tgccgagcgc cgacccctcg
     7021 cgcgccgctc gcgcggcttc gcgggtggac tgtccgcagc cgcagccgat gtcgagcacc
     7081 tgatcggaag cccggatggc cgcggcccgg ctcaggtgag gagcgaggct gcggaccgcg
     7141 tcgttgtacc agtgctcgtt gtcggtccag ttgtcgccct cgggtccgtc ccagccgcgc
     7201 gcctggtcga cgttcgcgat cgcgctcccc gatcccatcc cgcctcctca tcgagcaagc
     7261 tcgatgctat agtcccctag ggactatcat gccgtcaaga gggaattcgc ggccgcccgc
     7321 cggcttattc ggcgtgctgg gcctgctcgc ggtccgaccg atgagcacct acgagctcgc
     7381 aacgcacttc gaccggtcgc tcggccggat gtggccgcga acccgcagca agctgttcga
     7441 aatgccgaag cggctggtcg agatgggctt cgctcgtgcc gccaaaggtg cgaccggacg
     7501 gcgcgccaag accgtctaca cgatcacgcc gaagggccgt cgggcgctcg cggcgtggct
     7561 cgccgagccg ggcggcgggc cggtcatcga attcgaggcc ctgatgaagg tcttctacgg
     7621 cgaccagggc ggtcgcgacg cgatgatcgc caatctcgag gcggccaagg actgggcgcg
     7681 agagcagatc gacgagcaca tcgaggtggc gcggacctac ctcgagggtc gcggtgcgtt
     7741 ccaggatcgc gcggccatca accagattac cggccggttc cacgtcgagc ttgcgctcac
     7801 cgttcatcga tgggccaccc gctccttggc gaccgttcgc acgtggccgg cggatccgcg
     7861 tgaagccgag atgagccgcg cggcgctcga ggacgtgctc cgcgagctgg aagtggcacg
     7921 ggaatgagaa agcggggccc gatcgggccc cgctttcgtt caggtgtctt agtgtccgtg
     7981 cgcgtgtccg tcggaaacgc gagtctctcg cgtgccgaac caggatgacc agaacagcgc
     8041 cgacagcagc agcgccacgc cacccacgat catcaagatc actccgatgg ttccgatgtt
     8101 gaatccttcg gcgtctgcgg tgaccgcgaa gcgaaggatc gcacccaacg cgatgagcac
     8161 aactccgatc ccgataccgg gcatcgatat ctcctttctt cgggtcgtct cgtgcttggt
     8221 gtagatcccc actcccggcc tcgccgaaac ccgctccgcg gatgcgagga ttcgtcggat
     8281 cgatcaatgc gggcctcgaa aattgtcgaa tcgctcgcag gagccgcctt ccctttgagc
     8341 gaagttgatc ggaaatgaga caaaaccggg agggaagcat gctcaagcga ttgttcgtct
     8401 ccgccgtcgc ggtggcgatg ctggtgccgt tcggcggcgc gcaggccgca ccgatcgaca
     8461 cgtggccagt cacccacggc atcgactgtc tgaacgatcc gttcaacccg acccgctcgg
     8521 acggcaccaa ggacccggcc ggcgatccgc tgcccggcac ggccgcctgg atcgagcgcg
     8581 accggcaacg gctggaatgc gacaaccaac gtgatcacga ccgccgttac cacccccaca
     8641 acagcttgaa ctcgggccgg tacggcgagg actggtaccg caacccgcat cgcttcgacg
     8701 gccgtcgctg gcgctacgac tacattgccg ccgacgcgca gggtctgtac ggcatcccgg
     8761 gtgttcccgc ggccgagatc tttcgcccgt gcaagccgct cacgtgtctg aacaagccgg
     8821 ccgagctcga gcgcttcgcg ccgccgtacc cggtcgtggt gatccatcac ggattcatcg
     8881 cgcagatgac gcatcaccgc ttcaacgcgc aggtctacgc cgaggccggc tatatggcga
     8941 tcgtcgtcaa cggcatccac ccggtgaccg gtgtgccgaa cgtgcaggac tccgcgaacg
     9001 gcgcgcaggt cctggactgg ctcgcatcgc ccgccagcgg cgagatcggc cgccaggcgg
     9061 acctgagccg cgtggcgatg gtcggtcact cgcagggcgc tgccgcggcg ctgagcaacc
     9121 agggcgatcc gcgcgtggat gcgatcgtcg cgtgggacgg cggagattcg atctcggatc
     9181 tgaactgcaa ggacgggaac ccgtgtgccc cggtcatgta tcagcgcacc gacggggcgt
     9241 tcagcgcgcc gcaggcgacc gcgcgcgccg ggtaccccgc gacgcgtgag cgtggcgtgc
     9301 ccacctacct cgagcataag gcgcgcgaca tgaacgttta ccacatcacg atgcgcgcct
     9361 cgaaccacat cgactggaac ggcaacggtg tcggctcgct ggccggcagc cgtctcgccg
     9421 agccgtggat caactactac acgctcgcct ggctcgacct gcatctgcgg gggcagctcg
     9481 ttcgtgacgg gaacggaaac atcgtcacgc aacacggccg ctcggcctgg gacgagcgct
     9541 ggttccgtca ggggatcgtc aaccgggcgt tcaacatgct cacgtcgaag cggtttctcg
     9601 gcgggacgat cgacaagcac aacatcagcc agggtttcta cgacccggtg cagatggcga
     9661 cgtcgggtga cccgctgttc ggcggaaacg ttccatacca gatcgaggac ctgtgggtgg
     9721 tggatcgcct ctcgcccgag ttccgcaacc tgtgctcgct cactgtgccg aactacgtca
     9781 cgggtggaac cctggccacg gccgacaccg gcgttgccgg cgacattcgc ttgaccggct
     9841 gccccgtgct gtagccgcac cctgcggcgg caggagccga gttgccggac gaaacctttc
     9901 ggcaactcgg ctctctgtct gcccgggtcc tgagccctcg acctgggcgg gggtgggggg
     9961 tgcctctcgg acaccctgtg caaccaagtt gcaagggcgt tgatgccgtt atccggtaga
    10021 tgttccgctc cggcacgctt ggggtggccg cgaagcggaa tgcgatcttt gcaacttcgt
    10081 tgcacagggt cgtggcaact caaggctttg ggggtatgga gccctgcgcc ggttcgggcg
    10141 cggcggtctc tgcctggcgg tctctgcctg acggtcggcc cggacggtcg gcccggtgtc
    10201 tggctgacgg ctagcggcgg tgctcggggt tggggaagtc ggggcggcag ccggcatccc
    10261 attcgctgcg ctggttgccg tgcgcgggga tgccgccggc gtctttgatc atgcgcgcga
    10321 ggtgcatcag gttccaggac atgaacgacg tgttgcggtt ggcgaagtcg ttctcgggac
    10381 cgcccgatcc gggatccaga taggacgggc ccggtccggc ctcgccgatc cagcccgcgt
    10441 cggcctgcgg cgggatggcg tagccgagat gctgcagcga atagaggatg ttcatcgagc
    10501 aatgcttgac gccatcctcg ttgccggtga tcaggcagcc gcccacccgt ccgtagtaca
    10561 ggtactgacc cttgtcgttg agctcacccg attgcccgta gaggcgctcg atgaccttgg
    10621 tgcattccga cgacttctcg ccgagccaga tcggtgagca cagcacaaga atgtccgcgg
    10681 cctggacctt cgcgaagatg ccgggccagt cgtcccggtc ccagccgtgc tcggtcatgt
    10741 cggggtagac gcccggcgga atgacgtgat cgaccgaacg gatcatctcg acgtccacgc
    10801 cgttgcgttg catgatctcg accgctatcc gtgcgaggcc ctcggtatgc gagacctggg
    10861 gcgacttctt gagcgtgcag ctgataaaca ccgcacgcag atcggagaag tcgcgcgggc
    10921 ggtctttggg cgcatcgttg cgttcgctgg gcgtcacgtt ccctcctcgg ccggccgctt
    10981 ctgtagtcct ctcggccgag atatcgttac acgtcccccg cgcgcgctcc ggcccgacac
    11041 cagcagcgtc ttcaggacgc gcacccacgt tcccggatga ccgaaaaatg agggtcgtac
    11101 cccttgaccg gcttttgccg cttcgagtag gttgcttgcc ttagtcagcc tcgaagtgtc
    11161 ccgatcacat ttgagagcgc gagacccccc ccgtcccggg cggctcctag cagagggagc
    11221 gccgtcctca tccaggacgc cccctcaccg tcttgccatt ccccgtcaag cagtccaccc
    11281 gtatctgctc aggcaggagg aacgaatgag gagcactcgc aagatcgcac tgctcgccgt
    11341 ggcggttctc gccgcgctca ccgtccgagt agcgcccgcc gacccggtca aggggctcgt
    11401 cgaagtcacc ggcacggtca tccccaacca gtacatcgtg gtgttccacg acacgctcgt
    11461 ctccgacgtc ggcgtggccg cgagcacgat ggcccgcgag aacgacgcgc gcatccagtt
    11521 cgtctatcag cacgcactca aaggtttcag cgccgagatg agcccggccg ccgccgcgaa
    11581 gatggcgctc gaccgccgcg tcgcatggat cgaacccgac caggaaatgg aactgatcgc
    11641 cacgcagtcc aacccgccgt ggggcttgga ccgcatcgac cagcgtgacc ggccgctcaa
    11701 ccagtcctac gtctacaacg cgaccggctc gggcgtaaag tcctacatca tcgacaccgg
    11761 aatccgccgc acgcatgcgg acttcggcgg ccgggcgatc gtcggctatg acgccatcac
    11821 gagcggcgga tccgcgaacg attgcaacgg tcacggtacc cacgtcgccg gatcggccgg
    11881 tgggaccacg tacggcgtgg ccaagtccac cacgctcgtc gcggtgcgtg tgctcgactg
    11941 caacggctca ggctcgaact cgggcgtgat cgcgggcgtc gattgggtca cggcggatca
    12001 cctggcgggt cagccggccg tggcgaacat gagcctcggc ggcggcgcct cctcggcgct
    12061 ggacacggcg gtgaacaact ccatcaacga cggcgtcacc tacgtggtcg cggccggaaa
    12121 cggcgacttc ttcggcaacc cgcagaacgc ctgcaactac tcgccggcgc gtgtcgccgc
    12181 cgcgatcacg gtcggcgcga ccgactccaa cgacgcccgg gcttcgttca gcaacttcgg
    12241 aacctgcctg gacatcttcg cccccggcgt gagcatcctg tctgcctggc acagcagcga
    12301 cacggcgacc aataccatca gcggtacctc gatggcgtcg ccgcacgtcg ccggagccgc
    12361 ggcgatgtac ctgcagggca acacgagcgc cagccctgcg accgtgagca acgcgctgac
    12421 cagcaacgca tcgagcggca aggtctcgaa cccggggacc ggctcgccga acctgctgct
    12481 ctataccgcg ttcatcggag gcggcggcgg caaccaagcg ccgacggcga acttcacgta
    12541 ttcgtgttcc ggcctgacgt gcaacttcac cgacacctcg accgactctg acggcacgat
    12601 ctcgtcgcgg tcgtggaact tcggtgacgg caccacctcc acggccacca acccctccaa
    12661 gacgtactcg gcggcgggca cctacacggt gacgctgacc gtgacggata acggcggcgc
    12721 gaccggctcg accagcaaat ccgtgacggt ctcgtccacc tcgagcccgt gcggagcgga
    12781 cccgaacacg tcgatcccga atctcacgaa cggcgtgttc acctcgggaa cggccgcgtc
    12841 tacgaccgtg cgttggag
//
LOCUS       DATLFO010000198        12742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_133480, whole genome shotgun sequence.
ACCESSION   DATLFO010000198 DATLFO010000000
VERSION     DATLFO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133480"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VM841_08090"
     CDS             complement(<1..1574)
                     /locus_tag="VM841_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVL90177.1"
                     /translation="MGIVAVLASVRRPNAKMRSLAVALGLLGTLPTLSLLSAAAPSQW
                     SAVIARASAVVFLSSGFALLVFRHRLIPLDRRVLWALGAASVAAAGAAIGTGLDAAGA
                     RGPAQMAAALSLMAVWVACVGEPTYRFWRTARLRPIIQRARLRALAAGYFGVIVVAVI
                     SRPSNDNHAIFLASQIVVAFVIPLLWIALHPPSWLRRSWQSRERISRAALDAMVRHIG
                     DERSLAARSLTWARTIVGGEGAALVDADGSVLGLDGIDEDTVRALRAAAPPGEAAMID
                     YNGGAVVAPVPLEAGSGSLIVTSGPLSPLFDAHETDEVAAFASTVAIALERVRTLARH
                     RDQAEHYAGVVQAMGDLGEGFLVTDGRRLLFANDAYCRLTGYSLEELMAMPSLEALAP
                     PDELERLQRRRTERLRGAAVVDSYDSALIRKDGERVELEVSMKILGTEDKPSIVCIVR
                     DITDRKRAEDRLRAALEREHDAVRRLESLDEMKNAFLSAVSHELRTPLTGVLGFARTL
                     QSLGDTMDQDDRTNVV"
     gene            1771..3012
                     /locus_tag="VM841_08095"
     CDS             1771..3012
                     /locus_tag="VM841_08095"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HVL90178.1"
                     /translation="MSDAWTTALSQLDEAAAHMRLDPNLHEVLRRPKRILTVSIPVRM
                     DDGRVEVFTGYRVHHDLSRGPAKGGIRYHPDVSLDEVKALAMWMTWKTAIVGIPYGGA
                     KGGVVVDPKRLSDGELERLTRRYASEILPFIGPERDVPAPDLNTDERVMAWIMDTYSM
                     NTGYSVPGVVTGKPVSVGGSLGRAGATSRGVMFAALMAIKQQGLSVDGLRVAVQGFGK
                     VGGFAAQLFHDVGCTVVAISDYKGGVYNARGLNPTAMLRYRDENGTVHGYPNGDAITN
                     EELIELDCDLLVPAAIEDQITQHNADRVRAGIVVEGANGPTTREADHILDDRGVLVVP
                     DVLANAGGVTVSYFEWVQDIAAYFWTEDEVNTRLRGLMERSYEQARAYAEEHGIRLRL
                     AALALGIGRVAEAHRVRGLFP"
     gene            3138..3428
                     /locus_tag="VM841_08100"
     CDS             3138..3428
                     /locus_tag="VM841_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HVL90179.1"
                     /translation="MRDDINVDGVRAPGELTEREREILAFEREWFKTPGSKERVVRER
                     FGVSATRYYQILGALIDSPEAVAHDPMLVKRLQRMREARKRRRAAQRLGVRE"
     gene            3439..3999
                     /locus_tag="VM841_08105"
     CDS             3439..3999
                     /locus_tag="VM841_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HVL90180.1"
                     /translation="MPGRHAPASSKSFLISLGTAVGGALVALAVVVGIAVVVLNAGSD
                     AEEPGAINTPPTTAPTTPPPATTAPTPTASPTPSPTETAEVTPVSETTLRILNGTRRA
                     GLAASMADRAKAEGYPAPKVGNSAPAAKSTIYYRADSQDEAEELRRRFPDLTEIAPAP
                     SSFQTDVMLTVVLGDDYPSPAPSPTA"
     gene            complement(3989..5056)
                     /locus_tag="VM841_08110"
     CDS             complement(3989..5056)
                     /locus_tag="VM841_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90181.1"
                     /translation="MKTGWGKTAAVRTGFVLVLSVGAAACASPAPVLSPELPHGELRV
                     YRMTTEAVTTSSIPGLGGKDVTVLAADLRIEVGRINGKRGALLAVVPISFVRNGAKGE
                     VPPRQDAAVVFDDDGAVESVVGPKGKPAAFGGSFDDIGTIFGSTIAKGPVKVAERWAS
                     RVGSGPQATTRRSRVAALRVVKGYRVAIVESAVRRPVDRTRTVGGNQLRLVGSEVSNV
                     TTAFAFDFGLPVEIVSAATTEMQIAGAPQRGTVTIMTTTKIGLIGRSDGTPRQLPSVP
                     TPAPSTSPAAGPTASPKPSATPSAPASPKRSPSKSPTPTKKPTSTPKTGSGGGSGAEP
                     ISPDPPPQDPTPGAVGPVARP"
     gene            5110..5889
                     /gene="otsB"
                     /locus_tag="VM841_08115"
     CDS             5110..5889
                     /gene="otsB"
                     /locus_tag="VM841_08115"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVL90182.1"
                     /translation="MQPRDHPSLEAFRAVPPGRAGIFTDFDGTLSPIVPRPEDARPQR
                     GAGPVLARLARRYAVVAIVTGRAASDVGRRLRAPGVRIVGVHGLEEKFGRRVRVMPDA
                     DAARRTIEDAAVSLERRLASFDGIVVERKGLGLAVHMRNAPDPDAAERAVTEIVAGVA
                     REAGLAAHGGRRVVEIRPLLPTDKGTAIVRLAREAGIEAALFCGDDIGDLVAMHAVRS
                     LPVSSVVGVASPESPAELREAADVIVDGPADVVRILRRLTD"
     gene            complement(5886..7322)
                     /locus_tag="VM841_08120"
     CDS             complement(5886..7322)
                     /locus_tag="VM841_08120"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVL90183.1"
                     /translation="MSERSIEQASLVFVSNRGPIQFDDDGVPHRGAGGLVTVLSSALI
                     ERQSAWVAAAMSKGDRLRTGLLSPEQTGLDDVWVRMLDIPAKDYDPYYNRLSNRVLWF
                     VNHYLFDPPRTPIFDEDDMASWDAYVAVNRRFAEAVADTAPEGASVFVQDYHLALAPA
                     TIRDLRPDLKIALFWHIPFAQPDYLRMLPDEWSRALLDGMLSADLAGFQTSRWADAFR
                     WGCKDLLGARTGEKWVKHAGNRTRIGVYPVGINGKHLLAAAASPDVGAERKALADWAG
                     GRRFILRVDRTELSKNILRGMLAFEALLDRRPDLRGRVAHMALMAPSRGNVPEYAEYT
                     EQCVAAADRINKRYGADTVRVDIENAIDRAYAAYGLYDVLVVNPVFDGMNLVAFEGPI
                     LNRRDGQLVLSQNAGAYTRLAPAAYGVNPFSIQATAAAIGAAIDAPADERRERAKRLR
                     RLARSHRPARWLLAQIRDVQRAAEERQG"
     gene            complement(7319..7645)
                     /locus_tag="VM841_08125"
     CDS             complement(7319..7645)
                     /locus_tag="VM841_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90184.1"
                     /translation="MGNIEINAFQRSANVIVQKSLGEGFGLTVAEARWKSKPVGGSCG
                     DLRAQDRRTGYLVNVEECAGRAVTLLHDSTATAEMGAAGREVVRERLLSPRDIDDFSE
                     MLEVLS"
     gene            complement(8028..8873)
                     /locus_tag="VM841_08130"
     CDS             complement(8028..8873)
                     /locus_tag="VM841_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVL90185.1"
                     /translation="MFPIFEPPPGVAVVYSDLDGTMLGAAGSFVHDHDGVHTLEPARA
                     LLGALAAGIEIVPCSGRAMPGIAGDARILGMQTAIGEMGAVVSYQGGREVVEHLGAYP
                     GGAKNPFELMHICGAVDLIVERFRMEPHGPWARNREYTALMRGLADTDEVNAALDEAG
                     MGWCALVDNGVLHAGYLGLPPRTAHVYHLLPRGVSKGAAVAADRARREIPRERSVALG
                     DSLADLAMASEVAVVAITADALADDPNLAAAAAAVPNATVTARPGNLGWTDAINAIVA
                     SATGS"
     gene            8959..10188
                     /gene="thrC"
                     /locus_tag="VM841_08135"
     CDS             8959..10188
                     /gene="thrC"
                     /locus_tag="VM841_08135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVL90186.1"
                     /translation="MGAIEGLRCRECGRDYPAAALHVCEFCFAPLEVVYDYDAIRARL
                     SRERIKQGPRSMWRYTDLLPGSDEHRVDIGAGCTPLRPAPRLAAELGLRELWIKNDTA
                     NPTHSFKDRVVSVALTVAREFGYTVVACASTGNLANAVAAHAANAGLEAYIFTPYDLE
                     PAKIIGTAIYGPKLVAVKGTYDDVNRLCSEIAGEYPWAFVNVNIRPYYAEGSKTLAFE
                     VAEQLGWRAPDHVVAPMASGSLLTKIKRGLDELVTVGLLDGHATRISGAQPAGCSPIS
                     SAFKAGVDHIRPVRPDTIAKSLAIGNPADGYYAIKAVRETGGGMDDVSDAEVVEGISL
                     LARTEGIWAETAGGVVIGTLKKLCEAGIVGPEERTVALITGDGLKTVDAVADVARPAM
                     TIAPSLDDFSTAANLDG"
     gene            10208..10483
                     /locus_tag="VM841_08140"
     CDS             10208..10483
                     /locus_tag="VM841_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HVL90187.1"
                     /translation="MSVKVRIPTPLRTMTGGASEVGVEAASVREMIAALEAAHPGFAA
                     RICEDTGEIRRFVNVFVGDEDIRFLQGLDTPLQQGASVSIIPAVAGG"
     gene            10557..11030
                     /locus_tag="VM841_08145"
     CDS             10557..11030
                     /locus_tag="VM841_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HVL90188.1"
                     /translation="MLRFSLRTEYGLRALMELASRRGEGPVPAREIATHQRIPLRFLE
                     HQLASLHKAGIVASTRGAGGGCELARDPSEVRVSEVVEVLEGPLSAMFCLDPHDESCP
                     QTHHCGLQELWTKVEAAVRDVFERTTIAELVERHVQMQPLLWPVMMPAPSASRAN"
     gene            11169..11474
                     /locus_tag="VM841_08150"
     CDS             11169..11474
                     /locus_tag="VM841_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HVL90189.1"
                     /translation="MPAKKRAAKKRTAAKKTTAKKTAARKPAKKRTTAKKTAARKPAA
                     KKTAARKPAKKRTAAKKRTAAKKTTARKTTAAARKTTAKKTTARKTTRRKSAAKKKR"
     gene            11739..>12742
                     /gene="groEL"
                     /locus_tag="VM841_08155"
     CDS             11739..>12742
                     /gene="groEL"
                     /locus_tag="VM841_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HVL90190.1"
                     /translation="MAAKLIAFDTEARRALEKGMDTLADAVRITLGPKGRNVVIEKKW
                     GAPTITNDGVSIAKEIELEDPIERIGAELVKEVAKKTNDVAGDGTTTATVLARAMVRE
                     GLKNVAAGANPMSLKRGIEKAVDRVVEHIKAAAKEIEDKDEIAHVASISANNDPEIGS
                     MIAESMDKVGKDGVITVEESQTFGLELELVEGMRFDKGYISPYFVTDPERMEAALEDP
                     YILIANSKISAVKDLLPILEKVMQTGKALVIIAEDVEGEALATLVVNKLRGTLQAAAV
                     KAPGFGDRRKAMLEDIAILTGGRAITEDLGIKLENIKLEDLGKAKKITIDKDNTTIVE
                     G"
BASE COUNT         2075 a         4343 c         4453 g         1871 t
ORIGIN      
        1 tccactacgt tcgtgcggtc gtcctgatcc atcgtgtccc cgagcgactg caacgttcgc
       61 gcgaatccga gcacgccggt gagcggcgtg cgaagctcgt gcgagacggc ggacagaaac
      121 gcgttcttca tctcgtccag cgattcgagc cggcggacgg cgtcatgctc tcgctcgagc
      181 gccgcacgca gccggtcctc ggcccgtttg cgatccgtga tgtcgcgcac gatgcagacg
      241 atcgacggct tgtcctcggt tccgaggatc ttcatcgaga cttcgagctc gacacgctcg
      301 ccgtccttgc ggatcagcgc ggagtcgtag gagtccacca ccgccgcgcc gcgcagtcgc
      361 tcagtgcggc ggcgttgcag ccgctccaac tcgtccggcg gcgcgagcgc ctccaacgag
      421 ggcatggcca tgagctcttc gagcgagtag ccggtcagcc ggcagtaggc gtcgttcgcg
      481 aagagcaggc ggcgaccgtc ggtaacgagg aagccttcgc ccaagtcgcc catcgcttgc
      541 acgacccccg cgtagtgttc ggcctgatcc cggtggcgcg cgagcgtccg gacacgctcg
      601 agggcgatcg cgaccgtgga cgcgaatgcc gcgacctcgt ccgtttcgtg cgcgtcgaac
      661 agaggggaaa gcggacccga cgtcacgatg aggctcccgc tccccgcttc cagcggaacc
      721 ggagcgacca cggcgccccc attgtagtcg atcattgcgg cttcgcccgg gggagcggcg
      781 gcacgcagag ctcggacggt gtcctcgtcg attccgtcga gcccgagcac cgatccgtcg
      841 gcgtcgacga gcgccgctcc ctcgccgccg acgatggtgc gcgcccacgt cagcgagcga
      901 gcggcgagcg agcgttcgtc gccgatgtgc cggaccatgg cgtcgagtgc cgctcgcgag
      961 atgcgttctc gcgactgcca ggaccggcgc agccatgatg gtggatgcaa tgcgatccac
     1021 agcagcggga tgacgaacgc gacgacgatc tgggacgcga gaaagatggc gtggttgtcg
     1081 ttggacggcc gcgagatgac ggcgacgacg atcacgccga agtacccggc ggccaaggcg
     1141 cggagccggg ctcgttgaat gatcgggcgc agccgcgcgg tgcgccagaa tcgataggtc
     1201 ggctcgccca cgcatgcgac ccacacggcc atcaacgaca aggcggcggc catctgcgcg
     1261 ggtcctcggg cgccggcggc gtcgagcccg gttccgatcg cggcgccggc cgcggcgacg
     1321 gatgccgcgc cgagcgccca cagaacccgg cgatccagag ggatgagccg gtgccggaac
     1381 accaggaggg cgaagcccga cgacagaaac acgacggcgc tcgcccgggc gatcacggcg
     1441 gaccattgcg atggagccgc ggcggacagc agcgacagcg tcgggagcgt ccctaggagg
     1501 ccgagcgcga cggcgagcga gcgcatcttt gcgttgggac ggcggaccga cgccagcacg
     1561 gcgacgatcc ccactcctgc gaatcctgcg gcgacgatgg cctgcaggac tctggccccc
     1621 tgttgcatac cctttctata tcggtccgga ccgggtgcgg ttaatagtca ttcacggctc
     1681 gcccttgctc cgggcatccg gcccgaaggg gacggagcac atggcccacc gggggccgga
     1741 gcagtgcgag gccgggatac actacgcagc atgagcgacg catggacgac cgccttgtcc
     1801 cagctggacg aggctgcggc gcacatgcgg ctcgatccca acctgcatga ggttctgcgc
     1861 cgcccgaagc gaatcctgac ggtgtccatc ccggtgagga tggatgacgg ccgcgtcgag
     1921 gtcttcaccg gctaccgggt ccatcacgat ctatcgcgcg gtccggcgaa gggcggcatc
     1981 cggtatcacc ccgatgtctc gctcgacgag gtgaaggccc tcgccatgtg gatgacctgg
     2041 aagaccgcca tcgtcgggat tccctacggg ggcgcgaagg gcggcgtggt ggtcgatccc
     2101 aagcggctct ccgacggcga gctcgagcgc ctcacgcgcc ggtacgcgag cgagatcctc
     2161 ccgttcatcg gccccgagcg cgacgttccc gcgcccgacc tcaacaccga cgagcgcgtc
     2221 atggcgtgga ttatggacac gtattcgatg aacaccgggt actcggtccc cggcgtggtg
     2281 accggcaagc cggtctcggt cggcggatcg ctcggacgag cgggcgcgac cagccgcggc
     2341 gtgatgttcg cggccttgat ggcgatcaag cagcagggcc tgagcgtgga cgggctgcgc
     2401 gtggccgtac aagggttcgg caaggtcggc ggattcgcgg cgcaactgtt tcacgacgtc
     2461 ggatgcaccg tcgtggcgat cagcgactac aagggcgggg tatacaacgc gcggggactc
     2521 aatcccaccg cgatgcttcg gtaccgggac gagaacggga ccgtccacgg ctatcccaac
     2581 ggcgatgcga tcaccaacga ggagctgatc gagctcgact gcgatctcct cgtgccggcc
     2641 gcgatcgagg accagatcac gcagcacaat gccgaccggg tccgggcggg gatcgtggtc
     2701 gagggcgcca acggtcccac cacgcgcgag gccgaccaca tcctcgatga ccggggcgtc
     2761 ctggtggttc cggacgtgct cgccaacgcc ggcggcgtga ccgtgtccta cttcgaatgg
     2821 gtgcaggaca tcgccgcgta cttttggacc gaggacgagg tcaacaccag gctgcgcgga
     2881 ttgatggagc gaagctacga gcaggcccgc gcctacgccg aagagcacgg catccggctg
     2941 cggctcgcgg ctctcgccct gggcatcgga cgggtggccg aggcgcatcg cgttcgaggg
     3001 ctgttcccgt gatgcccccg ggcgtgcggg aaaagtccac tccggccgcg ccgggtgaga
     3061 aagcaagccg agccgtcacg gagcgcgttc ccggttcccc ccgcgggggc gccgagtgtg
     3121 gcagaatcgg gcccgtcatg cgggatgaca tcaacgtgga cggggtccgc gcgccgggcg
     3181 agctcaccga gcgtgagcgc gagatcctcg cgttcgaacg cgagtggttc aagactcccg
     3241 gatccaaaga gcgcgtggtg cgtgagcgct tcggcgtctc ggccacccgc tattaccaaa
     3301 tcctcggcgc gctcatcgat tcgcccgagg ccgttgccca cgacccgatg ctcgtgaagc
     3361 gactgcagcg catgcgcgaa gcgcgcaaac gccgccgcgc cgcgcagcgc ctgggcgtcc
     3421 gggagtagaa ggagcctggt gcccggccgg cacgcgcccg cgtcgtcgaa gtcgttcctg
     3481 atctccctcg gaacggcggt cggtggtgcg ctcgtcgcgc tcgcggtcgt cgtcgggatc
     3541 gccgtcgtcg tcctcaacgc cggaagcgac gccgaagagc ccggcgcgat caacacgcct
     3601 cccacgacgg ctccgaccac gccgccgccg gccacgacgg cccccacgcc gaccgcctcg
     3661 cccacgccca gcccaaccga gacggccgag gtcacgccgg tgtccgaaac gaccctgcgg
     3721 atcctcaacg gcacgcggcg cgccgggctg gcggcctcga tggccgatcg cgcgaaggcc
     3781 gagggctatc cggctcccaa ggtcggcaac tcggcgccgg ccgccaaatc cactatctac
     3841 tatcgcgccg actcgcagga cgaggccgag gagcttcgac gccgcttccc cgacctcacc
     3901 gagatcgcgc ccgccccgtc gtcgttccag accgacgtga tgctcacggt cgtgctcggc
     3961 gacgactatc cgtcgcccgc tccgtcgcct acggcctagc gaccggcccg acggcgcccg
     4021 gcgtcgggtc ctggggcggc gggtccggcg atatcggctc ggcccccgaa ccgccaccgc
     4081 ttccggtctt gggcgtcgac gtcggcttct tcgtcggcgt cgggctcttc gacggagatc
     4141 gtttagggct cgccggcgcc gacggcgtgg cgctcggctt cggcgaggcg gtgggtccgg
     4201 cggccggcga ggtgctcggc gccggcgtcg ggaccgacgg cagctgacga ggagttccgt
     4261 ccgagcggcc gatgagcccg atcttggtgg tggtcatgat cgtgacggtg ccgcgctgcg
     4321 gtgcgcccgc gatctgcatc tcggtcgtcg cggccgacac gatctccacg gggaggccga
     4381 agtcgaacgc gaacgcggtg gtgacgttgg agacctcgct tccgacaagg cgaagctggt
     4441 tgccgccgac ggtccgggtg cggtccaccg gtcggcgaac cgccgactcc acgatcgcga
     4501 cccggtatcc cttgacgacg cgaagcgccg ccaccctgga ccgccgggtc gtcgcttgcg
     4561 gaccgcttcc gacgcgtgag gcccatcgct ccgccacctt gaccgggcct ttggcgatcg
     4621 tggatccgaa gatggtcccg atgtcgtcga aggacccgcc gaaggccgcg ggcttcccct
     4681 tgggccccac gacggactcg accgctccgt cgtcgtcgaa gaccacggct gcgtcctggc
     4741 gaggcggcac ctcgcccttc gcgccgttcc ggacgaacga gatcggtacg acggccagca
     4801 gcgccccccg cttgccgttg atgcggccga cctcgatgcg gaggtccgcc gccagcaccg
     4861 tcacgtcctt cccgccgagc ccggggatgc tgctggtggt cacggcctcg gtcgtcatgc
     4921 gatagacgcg aagctcgccg tgcggaagct ccggagagag cacgggcgcg ggcgacgcgc
     4981 atgcagcggc gccgacggac agcacgagca cgaacccggt ccgtacggcc gcagtctttc
     5041 cccaccctgt tttcatcgcc gagcagtcta gaacgagcaa gcgcgagcgc tggtagcgtg
     5101 gcgcagtcta tgcaaccgcg cgaccacccg tccttggaag ccttccgggc cgtgccgccc
     5161 ggccgggccg gcatcttcac cgatttcgac ggcacgctgt ccccgatcgt tccgcgaccg
     5221 gaggacgcgc gtccgcagcg gggagcagga ccggtactgg cccgcctggc ccggcggtac
     5281 gcggtcgtgg cgatcgtgac gggccgcgcc gcctccgacg tgggccgccg gctccgggct
     5341 cccggggttc gcattgtcgg cgtgcacggg ctggaggaga agttcggccg gcgcgttcgg
     5401 gtgatgcccg atgccgacgc cgcgcggcga accatcgagg acgcggcggt gagcctggag
     5461 cggcgcctcg cgagctttga cggcatcgtg gtggagcgaa agggcctggg gctggccgtt
     5521 cacatgcgca acgctcccga ccccgacgcc gcggagcgcg cggtgaccga gatcgtggcc
     5581 ggcgtcgcgc gcgaggccgg cctggccgcg cacggcgggc ggcgcgtcgt cgagatccgc
     5641 ccgctgctgc cgaccgacaa gggaacggcg atcgtgcgct tggcccgcga ggccgggatc
     5701 gaggccgcct tgttctgtgg cgacgacatc ggcgatctgg tggccatgca cgcggtgcgg
     5761 tcgctcccgg tgtcgagtgt cgtcggcgtc gcgagccccg agtcccccgc cgagcttcgg
     5821 gaagcggccg acgtgatcgt ggacgggccc gccgacgtcg tacggatcct gcggcggctg
     5881 acggactagc cctggcgttc ctcggccgcg cgctgcacgt cgcggatctg cgcgagcagc
     5941 cagcgggccg gccggtgaga ccgcgccagc cggcgcagcc gcttcgcgcg ctcgcgccgt
     6001 tcgtcggcgg gcgcgtcgat cgcggccccg atcgccgcgg ccgtcgcctg gatgctgaag
     6061 ggattcacgc cgtaggccgc cggcgcgagc cgcgtgtagg cgcccgcgtt ctgcgacagc
     6121 accagctggc cgtcgcggcg gttcaggatc ggtccttcga aggcgacgag gttcatgccg
     6181 tcgaagaccg gattgacgac cagcacgtcg tagagaccgt aggccgcata cgcgcggtcg
     6241 atggcattct cgatgtccac gcgcacggtg tcggcgccgt agcgtttgtt gatccggtcg
     6301 gccgcggcga cgcattgctc ggtgtactcg gcgtactcgg gtacgttgcc gcgcgacggg
     6361 gccatcagcg ccatgtgcgc gacgcggccg cgcaggtccg gccgccggtc cagcagcgcc
     6421 tcgaacgcga gcatgcctcg caggatgttc ttcgaaagct cggtgcggtc cacgcgaagg
     6481 atgaagcgcc ggcccccggc ccagtcggcc agcgccttgc gctctgctcc cacgtcgggt
     6541 gatgccgcgg ccgccagcag gtgcttgccg ttgatgccga ccggatacac cccgatgcgg
     6601 gtgcgattgc cggcgtgctt gacccacttt tcgccggtgc gcgcgccgag cagatccttg
     6661 cagccccatc ggaaggcgtc ggcccaccgg ctggtttgaa agcctgccaa gtccgccgag
     6721 agcatgccgt cgagcagcgc gcgcgaccat tcgtccggca gcatgcgcag atagtcgggc
     6781 tgtgcgaacg ggatgtgcca gaagagcgcg atcttcaggt cgggtcgcag atcgcggatc
     6841 gtcgcgggag ccagcgccag gtggtagtcc tgcacgaaca ccgatgcgcc ctcgggggcg
     6901 gtgtcggcga cggcttcggc aaagcgccgg ttgacggcga cgtaggcatc ccacgacgcc
     6961 atgtcgtcct cgtcgaagat cggcgtgcgc ggcggatcga acaggtagtg gttgacgaac
     7021 cacagcaccc ggttcgacag gcggttgtag tacgggtcgt agtccttggc gggaatgtcg
     7081 agcatccgga cccagacgtc gtcgagaccc gtctgctcgg gggaaagcaa gccggtgcgc
     7141 aaacggtcgc ccttgctcat cgcggcggcg acccatgcgc tctgccgctc gatcagcgcg
     7201 ctcgacagga ccgtgaccag tccgcccgct ccgcggtgcg gcacgccgtc gtcgtcgaac
     7261 tgaatgggtc cgcggttcga tacgaagacc aggctggcct gctcgatcga tcgctcgctc
     7321 acgacagcac ctcgagcatc tccgaaaaat catcgatatc gcgcggggac aagaggcgct
     7381 cgcgcacgac ctcccggccg gctgcgccca tctccgcagt cgccgtggaa tcgtgaagca
     7441 gcgtcacggc tcgtccggcg cactcttcta cgttcacgag gtatccggtt cgccgatcct
     7501 gggcgcgcag gtccccgcag cttcccccga cgggcttgga cttccacctc gcttccgcca
     7561 cggtcagacc gaatccttcc ccgagggact tctggacaat cacgttcgcg gatcgttgga
     7621 acgcgttgat ctcgatgtta cccacccgat ccacgctcga tagaaggtga acgtccgggt
     7681 cgcccgcggc gtgcccggcg cttgggggaa gcgggaccgc agtcagcatc gggtcaatgc
     7741 tagtcccgcg ggcgcgggag ggcgattccc cgctcgggtt gcatcggatg cccctagcgg
     7801 gcggtgagcg cgcgcctcgg ggccggccgg cacccgcgcc gaagccgcga gcgatgaggg
     7861 ttgcgggcga tctcgccccg tgccacggcg cgcgcgaagt cgcagacgac cggctgcttt
     7921 cgggccttgc gtcgtgtcgg gctttgcatc tcgtgctcag tatcggaacg gcggaccggt
     7981 cccttgaatg acgggatgtc gcaaatgtct atcgggggtg gacggagcta tgagccggtg
     8041 gccgaggcga cgatcgcgtt gatggcgtcg gtccagccca ggttgcccgg ccgagcggtc
     8101 acggtcgcgt tcggcaccgc ggccgccgcc gccgccaggt tcgggtcgtc ggccagcgcg
     8161 tccgcggtga tcgccaccac agcgacctcg gatgccatcg ccaggtcggc gagcgagtcg
     8221 ccgagcgcca cgctgcgctc acgaggaatc tcgcggcggg cgcggtcggc ggcgacggcc
     8281 gcgcctttcg atacgccgcg gggaagcaga tggtagacgt gcgcggtccg cggcggcagg
     8341 ccgaggtagc cggcatgcag cacgccgttg tccaccagcg cgcaccatcc catgccggcc
     8401 tcgtcgagcg ccgcgttcac ctcgtcggtg tcggcgagcc cgcgcatgag cgcggtgtac
     8461 tcgcggtttc gggcccacgg gccgtgcggc tccatgcgga agcgctcgac gatcagatcg
     8521 accgcgccgc agatgtgcat cagctcgaag gggttcttgg ctcccccggg atacgcgccg
     8581 agatgctcga cgacctcgcg gcccccctga tacgacacca ccgcccccat ctcgccgatc
     8641 gctgtctgca tcccgaggat tcgcgcgtcg ccggcgatgc ccggcatcgc ccggcccgag
     8701 cacggaacga tctcgattcc ggccgcgagc gcgcccagga gcgcacgcgc cggctcgagc
     8761 gtgtggacgc cgtcgtggtc gtgcacgaac gatccggcgg cgccgagcat ggtgccgtcg
     8821 aggtccgagt agaccacggc gacgccgggc ggcggctcga agatcggaaa cacgggcgaa
     8881 tcatacggat cggaggcggt gaaggccgga tcgcctatcg aaatgcgccg ggcgctcgaa
     8941 ttccctattc ttgcgtgcat gggcgcgatc gaggggctgc gctgccgcga atgcggccgg
     9001 gattatccgg ccgccgcgct gcacgtgtgc gagttttgct tcgcccctct cgaggtcgtc
     9061 tacgactacg acgcaattcg ggcgcgtctg tcccgcgaac ggatcaagca aggaccgcgt
     9121 tcgatgtggc gctataccga cctgctgccg ggatcggacg agcaccgggt ggacatcggt
     9181 gcgggctgta cgccgcttcg tccggcgccg cggctggccg ccgagctggg actgcgcgaa
     9241 ctgtggatca agaacgacac ggccaacccg acgcactcgt tcaaggatcg ggtcgtctcg
     9301 gtcgcgctga cggtggcgcg cgagttcggc tacaccgtcg tcgcctgcgc ctcgacgggc
     9361 aacctcgcca acgcggtcgc ggcccacgcc gcgaacgccg ggctcgaggc ctacatcttc
     9421 acgccctacg acttggagcc ggccaagatc atcggcaccg cgatctacgg gcccaagctc
     9481 gtggcggtca aaggaaccta cgacgacgtc aatcggctct gcagcgagat cgcgggggag
     9541 tatccctggg cgttcgtcaa cgtcaacatc cgtccctact acgcggaagg ctcgaagacg
     9601 ctcgccttcg aggtcgccga gcagctcggc tggcgggctc ccgaccacgt cgtggcgccc
     9661 atggcgagcg gatcgctgct gaccaagatc aagcgcgggc tggacgagct cgtcaccgtc
     9721 gggctcttgg acggccacgc gacgcgaatc tccggggctc agccggccgg ttgctcgccg
     9781 atctcgtcgg cgttcaaagc cggcgtggac cacatccgtc cggtccgtcc cgacacgatc
     9841 gcgaagtcgc tcgcgatcgg aaacccggcc gacggctact acgcgatcaa ggccgtgcgc
     9901 gagaccggcg gcggcatgga cgacgtcagc gacgccgaag tcgtcgaagg catcagcctg
     9961 ctggcccgga ccgaaggcat ctgggccgag accgcgggcg gtgtcgtgat cggaactttg
    10021 aaaaaacttt gcgaggccgg gatcgtaggt ccggaggagc gaacggtggc gctgatcacc
    10081 ggcgacgggc tcaagacggt cgacgccgtc gccgacgtcg cgcgacccgc tatgacgatc
    10141 gcgccgtcgc tggacgactt ctccacggca gcgaacctgg acggctgagc acggaggtgg
    10201 atgcgacgtg agcgtgaagg tgcgaatccc gaccccgctt cgaacgatga ccggcggcgc
    10261 gtcggaggtg ggggtcgagg ccgcgagcgt ccgggagatg atcgcggcgc tggaggcggc
    10321 gcacccgggc ttcgccgcgc gcatctgcga ggacacgggt gagatccgtc gcttcgtgaa
    10381 cgtcttcgtc ggcgacgagg acatccgctt cctgcagggg ctcgacactc cgttgcagca
    10441 gggggccagc gtgtccatca tcccggccgt ggcgggcggc tgatgccgcc ctccgttgct
    10501 ctccggcccg gggcgcgata gcatttttcc taggtaaaac aaaggatttt cggcacatgc
    10561 ttcgattctc gctccgcacc gagtacgggc tccgcgcgct gatggagctc gcctcgcgcc
    10621 gcggtgaagg cccggttccg gctcgcgaga tcgccaccca ccagcggatc ccgcttcggt
    10681 tcctcgagca ccaattggcg tcgctccaca aggccggcat cgtcgcttcg acgcgcggcg
    10741 cgggtggcgg ctgcgaactc gcgcgcgatc cgtccgaggt tcgcgtctcg gaggtcgtcg
    10801 aggttctcga agggccgctg tcggcgatgt tctgtctcga tccgcacgac gagtcgtgtc
    10861 cgcagacgca tcactgcggt ctgcaagagc tgtggacgaa ggtcgaagcg gcggtgcgcg
    10921 atgtgttcga gcgcaccacg atcgcagagc tcgtggagcg tcatgtgcag atgcagccgc
    10981 tgctgtggcc cgtcatgatg ccggcgcctt cggcgtcgcg cgccaactag aaacgcccgt
    11041 tcgccgctgc tgcgcgcgcg agaaaacctt ccgaaaacct tgaacatccc gaatttgctg
    11101 gttagagttc ccgcggttgc gtttctaagg tgaaacgcac ttctcccgaa cggaggaggt
    11161 ggcaccgagt gccggcgaag aagagagccg cgaagaagcg gacggccgcg aagaagacga
    11221 cggccaagaa gaccgcagcg cgcaagcccg cgaagaagcg gacgaccgcg aagaagactg
    11281 cggctcgcaa gcccgccgcg aagaagaccg cggcgcgcaa gcccgcgaag aagcggacgg
    11341 ccgcgaagaa gcggacggcc gcgaagaaga cgaccgctcg caagaccacg gcggccgcgc
    11401 gcaagacgac ggccaagaag acgaccgcgc gcaagaccac gcgtcggaag tctgctgcca
    11461 agaagaagag gtaacaggca tcactttccg ctaagagggc ctcgcacacg cgaggccctc
    11521 ttcgcgtccg cggaacggaa gctgcgggct ccggccgccg gccgaaaccc ggcaggactt
    11581 gcgaacgccc ccgccgtccc cctataaagt gcgttagcac tctcatatgg cgagtgctag
    11641 ccgggccggc cagctgtgag gcccggcccc ctggaaggaa ttggaaacga cccgaacggg
    11701 gcgatccccc gcggggcgga gtggaaaggg agggatggat ggcagccaag ctgattgcct
    11761 tcgatacgga ggcgcggcgg gcgctcgaaa agggtatgga cacccttgcc gacgcggttc
    11821 gcatcacgct cggccccaag ggccgcaacg tggtcatcga gaagaagtgg ggcgccccca
    11881 cgatcaccaa cgacggcgtc tccatcgcga aggagatcga gctcgaggac ccgatcgagc
    11941 ggatcggcgc cgagctcgtc aaggaagtcg ccaagaagac caacgacgtc gccggtgacg
    12001 gcaccaccac cgcgaccgtc ctggcccgcg cgatggtccg cgaaggcctc aagaacgtcg
    12061 cggccggcgc gaacccgatg agcctcaagc gcggcatcga gaaggccgtc gaccgcgtcg
    12121 tcgagcacat caaggccgcc gccaaggaga tcgaggacaa ggacgagatc gcccacgtcg
    12181 cctcgatctc ggccaacaac gacccggaga tcggctcgat gatcgccgag tcgatggaca
    12241 aggtcggcaa ggacggcgtc atcaccgtcg aggagtcgca gaccttcggc ctcgagctcg
    12301 agctcgtcga gggcatgcgc ttcgacaagg gctacatcag cccgtacttc gtcaccgacc
    12361 ccgagcgcat ggaagccgcg ctcgaggacc cgtacattct gatcgccaat tcgaagatct
    12421 cggccgtcaa ggacctgctg ccgatcctcg agaaggtcat gcagacgggc aaggcgctcg
    12481 tgatcatcgc cgaggacgtc gagggcgagg cgctcgccac gctcgtcgtc aacaagctgc
    12541 gcggtacgct gcaggccgcg gccgtgaagg cgcccgggtt cggcgaccgc cgcaaggcga
    12601 tgctcgagga cattgcgatc ctcaccggcg gccgcgcgat cactgaggac ctcggcatca
    12661 agctcgagaa catcaagctc gaggatctcg gcaaggccaa gaagatcacc atcgacaagg
    12721 acaacacgac gatcgtcgaa gg
//
LOCUS       DATLFO010000199        17305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_24728, whole genome shotgun sequence.
ACCESSION   DATLFO010000199 DATLFO010000000
VERSION     DATLFO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24728"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /gene="nuoL"
                     /locus_tag="VM841_08160"
     CDS             complement(<1..1945)
                     /gene="nuoL"
                     /locus_tag="VM841_08160"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVL90191.1"
                     /translation="MVGILGIVEQQHAEEAVNFTVNFFGEHAWVIPALPLLAAVLIMF
                     FGKKLPRKGVEIGIAAVVGSLVWAVGVFLNYAGKTAGPTIEKSLEWFPFGGGLSLELG
                     MLVTGITAVMFLLVTFVSLMVQIYSTAYMHDDKRYTWYFAVLNLFTASMLNLVIGNNL
                     IQMLIGWELVGICSYLLIGHWWEEHENSSAAIKAFVTTKTGDIPFVIGIFVLFAAGHT
                     FNIQELVHGAEGGEIATGTLMAGAILLFGGAMGKSAQFPLHVWLPDAMAGPTPVSALI
                     HAATMVTAGVFMIARLFPIFEASPATLGVVAVIAAITMLGAALLAMVQDDVKKVLAYS
                     TISQLAYMFAAHSVAAGNAAMFHLFTHGFFKALLFLGAGSLIHAVHSNNMSDMGGLKK
                     IMPTTYWTFLVGTLALSGIPPFAGFWSKDEILAEAFHNPHHLNEVFTYGIYGVGLLTA
                     FLTAFYMSRAFHLIFEGSYRGKGHPHESPPAMKYPLVALAVLSMVVGLIGIPGWQSGF
                     GAWTAVGGVTHVPDPDFLLIVVSVAVAVLGIGLGRKVYMPAPAREPLEKMGWFYKLLV
                     NKYYLDTFYWKAIVKPIRDSVSKAMYWLNQNVLDGAVNGAAYSAKAIGRGLYHGVDQK
                     VIDGAVNGAGVGMKKAAAVRKVIQ"
     gene            complement(1939..2244)
                     /gene="nuoK"
                     /locus_tag="VM841_08165"
     CDS             complement(1939..2244)
                     /gene="nuoK"
                     /locus_tag="VM841_08165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVL90192.1"
                     /translation="MNLALVLTLSALLFSIGVYGVLARRNAVLVLMSIELMLNSVNIN
                     LVAFGAHLQDALAAGQIFALFVIAIAAAEVGIGLAIVLLIFRNRETINVDEVDILKW"
     gene            complement(2241..2735)
                     /locus_tag="VM841_08170"
     CDS             complement(2241..2735)
                     /locus_tag="VM841_08170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVL90193.1"
                     /translation="MNGPEIIFTALAVIAAVAGILVVTTKNVVRGALYLVAVLGSIAA
                     IFMLLGAEFIGWTQVLIYVGAIVVLLLFGLMLTRAPIGAARSVDNQQRGLALVVSASV
                     FGVLTYLIWRGFGDKEIPLKQVVRTEDIGNSMFSNFILPFEAISILLLAALVGAIVLA
                     RRDK"
     gene            complement(2752..3522)
                     /locus_tag="VM841_08175"
     CDS             complement(2752..3522)
                     /locus_tag="VM841_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVL90194.1"
                     /translation="MTRRAVTQQYPHEVPDLPPRTRGVIALKEENCTVCMLCSRECPD
                     WCIYIDSHKEQVPPKTPGARPRTRNMLDRFAIDFSLCMYCGICVEVCPFDALFWSPHF
                     EYSETDINNLIHEMPTLAEWVNHVKPPPPLDEGAQPEPEADKEAKVFEAGGAVAAAPA
                     ASGAPAGTATTPAREAAPGPAAAGSAESTPAGEASAPAEAEAAAAKAEAPAEAKHDEG
                     PVEIDEETYQAELAAGKPERVARAMAKKAFVIKRRKQG"
     gene            complement(3678..4697)
                     /gene="nuoH"
                     /locus_tag="VM841_08180"
     CDS             complement(3678..4697)
                     /gene="nuoH"
                     /locus_tag="VM841_08180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVL90195.1"
                     /translation="MIPAIAESHNVFLGFDFSNPWVLLGLKLAAVMVFFLTAPLGVGY
                     AEHKILAHMQARLGPMEAGRFHGWAQLIADGVKFIQKEDIVPSAADKAVFVIAPAMVL
                     VPAIMLFVVLPLGPNGFWVENMDLGLFFALAISSISVIGMLAAGWASANKFSLIGAIR
                     GAAQLIAYELPLVLAGAAVAMQAGTLSLPGIVEAQQNYWFVFPQIFGFGLFVTASLAE
                     LSRPPFDMPIADSEIIFGHMTEYTGIKFAFFLLAEYAGIVALSILAVVLYLGGWQGLP
                     FINFIPGPLWTLAKVGALAFFIIWLRATYPRLREDQLQKMAWKYMIPIALVNILLTGI
                     FKVVF"
     gene            complement(4709..5878)
                     /locus_tag="VM841_08185"
     CDS             complement(4709..5878)
                     /locus_tag="VM841_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVL90196.1"
                     /translation="MSDPTMLPGGPGEGIALDVAGSSRFATEEMILNIGPQHPSTHGV
                     LRMVITLDGELIIDAEPVIGYMHRGFEKMAEVRDYRQIIALVNRHDWLSAFGNELGVV
                     LGVEALMEIEVPERAQWIRTLLAEWNRVLNHLMFMGSFGLELGAMTPMFYAFREREEI
                     QGLLEAATGGRMHFTYNRIGGLKEDLPRGFLKESSRLAKVVRHRLGDYEGLLLGNEIF
                     KKRTVGIGKLPAAVALEYGCTGPILQAAGVAEDSRKTEPYLKYSEVDFEVPVGENGDS
                     FDRFWVLYRRIEESLKIVEQCHDKIPAGPIMPGKMPKNIKPPEGWIYSRTENSLGETG
                     YYIVSRGGKEPWRMKMRTPSFSNVSMIPYLLRGVLVPDLISVLGSVFFVVGDVDR"
     gene            complement(5881..6369)
                     /locus_tag="VM841_08190"
     CDS             complement(5881..6369)
                     /locus_tag="VM841_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVL90197.1"
                     /translation="MSAKALIDHVKEATGAVEREEVTPQGQVALHVTPANWHVVAEHL
                     RRCPRCNFSMFNWLSAVDMEDDGFEVIAHLYSASSLQAVNLKTMVPREGGSVPTLTGL
                     WRGASFHERECWEMFGIDFAGHPHLVKLLLAEPFVGNPLRKEFVLMTREAKEWPGAKE
                     PE"
     gene            complement(6373..6912)
                     /locus_tag="VM841_08195"
     CDS             complement(6373..6912)
                     /locus_tag="VM841_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HVL90198.1"
                     /translation="MGVVDKVNLPQPVKFVFNWGRKYSLWVMNFGLACCAIELIAAST
                     SRHDFMRLGVIPVAHGPRQADLLVVAGTLTDKMAPALKRVWDQMPEPKYCISFGSCSN
                     CGGPFWDSYSVTKGVDQIVPVDVYVPGCPPRPEALLEGIIRLQTQIADESLKERFERQ
                     RREAIVVGGPVGPVDLSNR"
     gene            complement(6903..7319)
                     /gene="ndhC"
                     /locus_tag="VM841_08200"
     CDS             complement(6903..7319)
                     /gene="ndhC"
                     /locus_tag="VM841_08200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVL90199.1"
                     /translation="MTQYFWQYGTIGALAILGVLFVVIAFSANRLMRPRKPANDKYTT
                     YECGIDPVGEGWSQTQIRYYIYAFLFVIFDVESVFLFPWAVVFETFPRPGLLLVEMII
                     FILILAVGLLYAYKKKVLDWVEPSESRAIRESQPWA"
     gene            7595..9106
                     /gene="nrfD"
                     /locus_tag="VM841_08205"
     CDS             7595..9106
                     /gene="nrfD"
                     /locus_tag="VM841_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HVL90200.1"
                     /translation="MAKFGFAIDHRACIGCHACTVACKMENDVPLGVFRTWVKYIEKG
                     NFPEAKRYFSVLRCNHCEDAPCTNICPTSALFTRDDGIVDFDNSICIGCKACMQACPY
                     DALYLDPGTHTAAKCNFCAHRVEVNLEPACVIVCPTHAIIAGDLDDPISEISRFVARQ
                     ETQVRAPEQGTKPKVFYAGADEAAIDPTQTTVPDAYMWSEVNLLDGQLQPRIDEAEAR
                     ARTVYDVDHPMPWGWKVSAYLWTKSIGAGVALLAAAAMLRGYGQIPLLATFAPAIALV
                     MTVITGALLVADLKRPERFWMLILKGNPTSWLVRGAWVLGAYSALLVAWLGGRVLDLP
                     WLVDAVRWPAIPLSALAAAYTAWLFGQAEGRDLWQSPLLAPVLIAQATLVGAASLLLA
                     SLFAGGESTQVLMTRVLVASAVGWAALTVVEFSSHSTQQAAKAARNLIRGPYRVWFWT
                     GVALGMVLPAALGGLALATGSSGPMVAASIAALGGLVFYEHGFVKAGQSVPLS"
     gene            complement(9124..9816)
                     /locus_tag="VM841_08210"
     CDS             complement(9124..9816)
                     /locus_tag="VM841_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVL90201.1"
                     /translation="MRRFFPLMLAAVLGACSGAADQAAPPPAPASPTPAPSPSGPAFR
                     GAVEVIDADARAKMGTSWKPGCPVPLHDLRVLRMDHWTFEGTVARGEMIVHEDQAEAV
                     LGAFAEIFEARFPIANMVIANGYIGNDPARAQQNATSAFNCRPAVGSSTLSQHAYGLA
                     VDINPVQNPRVTSAGVTPPDGKAYVDRTQDRPGMIKPGDAVVRAFEKIGWFWGGNYSK
                     VKDYHHFSLTGA"
     gene            9981..12776
                     /locus_tag="VM841_08215"
     CDS             9981..12776
                     /locus_tag="VM841_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HVL90202.1"
                     /translation="MAQYPPPERWDDWVEYEGSAWPKKVERHYILVPTVCFNCESACG
                     QLAYVDRDTLEIRKIEGNPVHPGSRGRNCAKGPATLNQIQDPERILYPQRRAGERGSG
                     RWERVSWDTALDDIAERIADAIRNERRDEVVYHVGRPGEDGYADRVLRAWGVDGHNSH
                     TNICSSSARVGYALWMGHDRPSPDYANARFTLLISSHLETGHYFNPHAQRIIEGKLAG
                     AKIAVMDPRLSNTASRADYWMPTKPGTEAAVLLAMAKIILDEELYDAPFMRRWWNWDE
                     YMRAQRPQDEPTFENFLLALKQEYAQYTPEYAASLAGVSADMIVKVAREVGRAGSGFA
                     AHNWRAAAAGNLGGWQVARCLFFLNALTGSVGTKGGTSPNWWDKFVPQPFLAPPPQKV
                     WNEILWPREYPLAHHEMSILLPYFLKERRQKLSVYFTRVYNPVWTNPDGFSWIEALSD
                     ESMIGLHAALTPTWSETAWFADYVLPMGLGPERHDTHSYETHSGKWLGFRQPVLRVAL
                     ERMGKPVQYTYEANPGEVWEEDEFWIELSWRIDPDGSLGVRQWFESPYRPGEKVTIDE
                     YYQYMFEHNVPGLPEKAQAEDLTPLEYMRKYGVVEIAPTVYEVHENPVPESALEGTTV
                     DPVTGLATKPGEPPSGKPIPLIGPWNAPGVEIDGVVREGFLTPSRKLEFYSPTMAEWG
                     WPEQALPNYMESHVASTDPKGGTYILMPTFRLPTLIHTRSNNSKWLNEISHRHPLWIH
                     PEDAERVGVRDEDLVKVITRIGHFVIRAWVTEGIRPGVVAASHHMGRWRLDRGFGTDR
                     WSSALVDLTYENASTEGATWRIRQKEHVQPFESKDKDSSRVWWTDAGVHQNLTFPTQP
                     DPVSGMHCWHQVVRLEAGGPDDQYGDIVVDTSKSFEIYKEWLAMTRPAPGPGGLRRPL
                     WMFRPLKPDASTYKIPD"
     gene            complement(12791..13276)
                     /locus_tag="VM841_08220"
     CDS             complement(12791..13276)
                     /locus_tag="VM841_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HVL90203.1"
                     /translation="MAEDNNKLSIVAFSGELDKLWPTMILATTASASGMQVSIFFTFW
                     GLFALAKPGVRITGSNWMQKGFSLVNPPGMKRAKLSKLHFGGAGKKMMLKLAKDYKVA
                     APWELMEYAQDMGVKLMPCQMTMDMLGLKREDLIDGLEPPLGAASALLEMKESSVQFF
                     I"
     gene            13515..13850
                     /locus_tag="VM841_08225"
     CDS             13515..13850
                     /locus_tag="VM841_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL90204.1"
                     /translation="MDVSVRNEIYELHARLCKALADPKRLLIINALRDGQKSVGELTA
                     ELELSQSNVSQHLAVLRERGVVAATRDGNNVFYTLKDVRVVEALDLLRSVMASQLDRR
                     SELRPIATG"
     gene            complement(13855..14322)
                     /locus_tag="VM841_08230"
     CDS             complement(13855..14322)
                     /locus_tag="VM841_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HVL90205.1"
                     /translation="MLEPAPEEIHYGCVIPRDLLYDVEADVWIRLEGDEAVIGMTDPA
                     QTRCGKLVAVDFRRVGKTISRGRGLATIESAKWVGPVASPLSGEIVAVNTETFERDIL
                     IANRDPYGEGWLVRLRPNSLDEEKGTLQEWSDAFAAYKQRIDELEIRCFRCAD"
     gene            complement(14329..14766)
                     /gene="gcvH"
                     /locus_tag="VM841_08235"
     CDS             complement(14329..14766)
                     /gene="gcvH"
                     /locus_tag="VM841_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVL90206.1"
                     /translation="MTAVRNCNIPDDLLYLVKNHVWARRDGDLVVCGITDVAQNLAKS
                     FISFTPKKVGKSVVKGKSTATLESGKWVGPVPAPVAGEIVEINEAGQSDPTIFNRDPY
                     GDGWVAKIKPEDWDRDIADLLTGQAALDAYEAFMQEEGIECGG"
     gene            complement(14763..15080)
                     /locus_tag="VM841_08240"
     CDS             complement(14763..15080)
                     /locus_tag="VM841_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90207.1"
                     /translation="MSCPYCDFEGARSALHRHLADEHPEKVTTRVDDKSGRRFYEVLC
                     PVCDAPWMREIKPRLRDPRFIEKFEEEIKLVAFDMLLYHIQAEHLLAEAGLEAEPTGE
                     GTP"
     gene            complement(15061..15546)
                     /locus_tag="VM841_08245"
     CDS             complement(15061..15546)
                     /locus_tag="VM841_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVL90208.1"
                     /translation="MTDYDAGALADGLWKRLRDVILVAERVDTFVFGPGDTGELPKVI
                     LTEQEAATVATDFVQRALAAACDRTNFRILEAASADDGIAAADLAATLSLPVLVVSER
                     VGDLLQTGLVSKALDTGRVYATAAGMQTVALVRDVAGRLAKTALKARGENGSNHELPV
                     L"
     gene            complement(15543..>17305)
                     /locus_tag="VM841_08250"
     CDS             complement(15543..>17305)
                     /locus_tag="VM841_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVL90209.1"
                     /translation="YIAYRRRFVKPFALDYTRVDKDSADYSRKPYKRGDWLFWHFLFW
                     LGVGGFLLEGLRIHANNFPAYEVWSPVGWLTARAIRGLGMSAATATDAHLYTWWAHAA
                     MALIFIGYIPFSKAMHMLTDAANLTFAERQSTLTLPRVPAGASAGIKSISDFTWKELL
                     DFDACTKCGRCHEVCPARTAGAPLSPRDLILDLRQFAEQSNRIRPIMDWERRAANGYT
                     GDGPPPVAGGIIPAETLWACTTCMACVEVCPVGIEHVPTIVGMRRSLVEEGTMDPTLQ
                     DALQNIAKNGNSFGKSPKMRAKWTKELDFEIHDARKRFVQVLWFVGDYASYDERLQDL
                     SRTFARLLQRAGISFGILYDGERNAGNDVRRVGEEGLFEELVEHNVKVINGCDFNEIV
                     TTDPHSFNTLKNEYPEFGGDWRVLHYTHLLHRLLEQGRIVPKQGLSMRVTYHDPCYLG
                     RYNGVTNAPRKILEMLGIELVEMPRNKENTFCCGAGGGRIWMDDSNQKERPSENRIKE
                     AVALGVDYFIVACPKDVTMYRDAVKTTGNEDKLQVLDIVELVERAVVGAPEPALVGVV
                     EGAGTVDVIDVAEVPAGGAE"
BASE COUNT         3009 a         5693 c         5733 g         2870 t
ORIGIN      
        1 gctgaatcac cttgcgcacc gcggcggcct tcttcatgcc gacgccggcg ccgttgaccg
       61 ctccgtcgat caccttctga tcgacgccgt gatacaggcc gcggccgatg gccttggccg
      121 agtacgcggc gccgttgacc gctccgtcga gcacgttctg gttgagccag tacatggcct
      181 tcgaaaccga gtcgcggatc ggcttgacga tcgccttcca gtagaaagtg tccaggtagt
      241 acttgttgac cagcagcttg tagaaccagc ccatcttctc gagcggctcg cgggcaggcg
      301 ccggcatgta gaccttgcgg cccaatccga tgccgagcac agcgacggca accgacacca
      361 cgatcaaaag gaagtcggga tccggcacgt gcgtgacgcc gccgacggcg gtccaggcgc
      421 cgaagcccga ctgccagccc gggatgccga tgaggccgac gaccatcgac aggaccgcga
      481 gcgcgacgag cgggtacttc atcgccggcg ggctctcgtg cgggtgtccc ttgccgcggt
      541 aggagccctc gaagatcagg tggaaggccc gcgacatgta gaacgcggtg aggaatgcgg
      601 tcagcaggcc gacgccgtag atgccgtagg tgaagacctc gttgaggtga tgcgggttgt
      661 ggaacgcttc cgcgaggatc tcgtccttgg accagaaccc ggcgaacggg ggaatccccg
      721 aaagggcgag cgtgccgacc aggaaggtcc agtaggtcgt gggcatgatc ttcttcagac
      781 cgcccatgtc gctcatgttg ttggaatgca cggcatggat cagcgaaccg gcgccgagga
      841 acagcagcgc tttgaagaag ccgtgcgtga acaagtggaa catggcggcg ttgccggccg
      901 cgacgctgtg ggctgcgaac atgtacgcga gctgcgagat ggtcgagtac gccaggacct
      961 tcttcacgtc gtcctggacc atcgccagca aggccgcgcc cagcatcgtg atggccgcga
     1021 tcacggccac gacacccagg gtcgcaggcg aggcctcaaa gatcgggaac agtcgcgcga
     1081 tcatgaacac gcctgcggtg accatggttg ccgcgtggat cagcgccgag accggggtcg
     1141 ggcccgccat ggcgtcgggc agccacacgt gaagcgggaa ctgggcgctc ttgcccatcg
     1201 cgccgccgaa cagcaggatc gcgcctgcca tgagcgtccc ggtcgcgatc tcgcctccct
     1261 cggcgccgtg cacgagctct tggatgttga aggtgtgtcc ggccgcgaac agcacgaaga
     1321 tgccgatcac gaaggggatg tccccggtct tggtggtgac gaacgcctta atcgcggcgc
     1381 tggagttctc gtgctcctcc caccagtggc cgatcagcag gtacgagcag atgcccacca
     1441 gttcccagcc gatcagcatc tggatcaggt tgttgccgat gaccaggttg agcatcgagg
     1501 ccgtgaacag gttcagcacg gcgaagtacc aggtgtagcg cttgtcgtcg tgcatgtacg
     1561 ccgtggagta gatctgcacc atcagcgaca cgaacgtcac gagcaggaac atcaccgccg
     1621 tgattccggt gaccagcatg cccagctcga gcgagaggcc gccgccgaag gggaaccact
     1681 ccagcgactt ctcgatcgtc ggtccggcgg tcttgccggc gtagttcagg aagaccccga
     1741 cggcccagac cagcgagccc accacggcgg cgatgccgat ctcgacaccc ttgcgcggca
     1801 gcttcttgcc gaagaacatg atcagcacgg ccgccagcaa cggcagcgcc gggatgaccc
     1861 aggcgtgctc tccgaagaag ttgaccgtaa agttcacggc ctcttcagcg tgctgctgct
     1921 cgacgatgcc caggatgcct accacttgag gatgtccacc tcgtccacgt tgatggtctc
     1981 cctgttccgg aagatcagaa ggacgatcgc caggccgatg ccgacctcgg cagcggcgat
     2041 ggcgatcacg aacagcgcga agatctggcc ggccgcgagc gcgtcctgga ggtgcgcgcc
     2101 gaaggcaacc aggttgatgt tgaccgagtt cagcatcagc tcgatggaca tcagaaccag
     2161 cacggcgttc cggcgggcca agacgccgta gacgccgatc gagaacagca gcgccgaaag
     2221 cgtcagcacg agcgcgaggt tcacttgtcc ctcctcgcaa gaacgatcgc gccgacgagc
     2281 gcagccagca gcaggatgct gatcgcctcg aacggcagga tgaagttcga gaacatgctg
     2341 ttgccgatgt cctcggtgcg cacgacctgc ttgagcggaa tctccttgtc gccgaagccg
     2401 cgccagatca gataggtcag aacgccgaac accgacgcgg aaacgaccaa cgcgaggcca
     2461 cgctgctggt tgtccaccga gcgggcggcg ccgatcggcg ccctggtcag catcagaccg
     2521 aacagcagaa gcaccacgat cgcgccgacg tagatcagga cctgcgtcca gccgatgaac
     2581 tcggcgccga gcagcatgaa gatcgcggcg atcgagccga gcacggcgac gagatacagc
     2641 gcgccgcgga ccacgttctt ggtcgtgacg acgaggatgc cggcgaccgc ggcaatcacc
     2701 gcaagcgccg tgaagatgat ctccggaccg ttcatctccc cccgtccccg cctacccttg
     2761 cttccgccgc ttgatcacga atgccttctt ggccatcgcg cgtgcgacgc gctcgggctt
     2821 cccggccgcg agctcggcct ggtatgtctc ctcgtcgatc tcgaccggac cctcgtcgtg
     2881 cttggcctcg gccggcgctt ccgccttcgc ggcggccgcc tcggcttcgg ccggtgccga
     2941 cgcctcgccc gcgggcgtgg actcggccga tccggccgcg gcgggcccgg gggccgcctc
     3001 gcgtgccggc gtggtcgcgg ttccggcagg ggcccccgac gcggccgggg ccgcggcgac
     3061 ggcgccgccg gcctcgaaga ccttcgcctc cttgtccgcc tcgggctcgg gctgcgcgcc
     3121 ctcgtccagc ggcggcggag gcttcacgtg gttgacccat tcggcgagcg tcggcatctc
     3181 gtggatcagg ttgttgatgt cggtctcgga gtactcgaaa tgtggggacc agaacagcgc
     3241 gtcgaacggg cagacctcga cacagatgcc gcagtacatg cacagggaga agtcgatcgc
     3301 gaagcggtcc agcatgttgc gggttcgcgg acgggcgccc ggcgtcttgg gcggcacctg
     3361 ctccttgtgc gagtcgatgt agatgcacca gtccgggcac tcgcggctgc agagcatgca
     3421 gaccgtgcag ttctcctcct tcagcgcgat gacgccgcgc gtgcggggcg gcagatcggg
     3481 cacctcatgg gggtactgct gcgtgaccgc cctgcgtgtc atcgtcttga gggtgatgct
     3541 cagccccttg gccagcccgt aggcgggacc gatgaccttc ttctcggcga tcttcttctc
     3601 gatctccttg aaacgcgcga gcatctgctc cttgcgcgtg ggctgaaccg gctcgagctc
     3661 cttcttctcg atggccacta gaacaccacc ttgaagatgc cggtcagcag gatgttcacc
     3721 agcgcgatcg ggatcatgta cttccaagcc atcttctgca gctggtcctc gcgaagacgc
     3781 gggtacgtgg cgcgcagcca gatgatgaag aacgcgagcg caccgacctt cgcgagcgtc
     3841 cagagggggc cgggaatgaa gttgatgaac ggcaagccct gccatccgcc caagtacagc
     3901 acgaccgcga ggatcgagag cgcgacgatg ccggcgtact cggcgagcag gaagaaggcg
     3961 aacttgatgc cggtgtactc ggtcatgtga ccgaagatga tctcggagtc ggcgatcggc
     4021 atgtcgaacg gcggccgcga cagctcggcg agcgacgcgg tcacgaacaa gccgaagccg
     4081 aagatctgcg ggaagacgaa ccagtaattc tgctgagcct cgacgatgcc gggcagcgac
     4141 agcgttcccg cctgcatcgc gacggccgcg ccggccagca ccagcggcag ctcgtaggcg
     4201 atcagctggg cggcgccgcg gatcgcgccg atgagcgaga acttgttggc gctggcccac
     4261 ccggcggcga gcatgccgat cacgctgatc gaggagatcg cgagcgcgaa gaacaggccc
     4321 aagtccatgt tctcgaccca gaagccgttc gggccgagcg gcagcaccac gaagagcatg
     4381 atcgccggca ccagcaccat cgcgggcgcg atcacgaaca ccgccttgtc ggccgccgac
     4441 ggaacgatgt cttccttttg gatgaacttc acgccgtcgg cgatcagctg ggcccagccg
     4501 tggaaccggc cggcctccat gggaccgagc cgcgcctgca tgtgggcgag aatcttgtgc
     4561 tcggcgtagc ccacgcccag cggcgcggtc aggaagaaca ccatcacggc cgcgagcttg
     4621 agcccgagca gcacccaggg gttggagaag tcgaacccga ggaacacgtt gtgcgattcg
     4681 gcgatcgcgg ggatcatcga aaggtgggtc atcggtccac gtctccaacc acgaagaaca
     4741 ccgaacccag caccgagatc aggtccggca ccaacacgcc gcgcagaaga tacgggatca
     4801 tcgacacatt cgagaaagag ggggtgcgca tcttcatgcg ccacggctcc ttcccacccc
     4861 gcgagacgat gtagtagccg gtctcgccga gggagttctc ggtccgcgag tagatccatc
     4921 cctccggcgg cttgatgttc ttgggcatct tgcccggcat gatcgggccg gccggaatct
     4981 tgtcgtggca ctgctcgacg atcttgagcg actcctcgat gcgccggtag agcacccaga
     5041 agcggtcgaa cgagtcgccg ttctcgccga ccggcacctc gaagtcgacc tcgctgtact
     5101 tgaggtaggg ctcggtcttg cgcgagtcct cggcgacgcc ggcggcctgc aggatcgggc
     5161 cggtgcagcc gtactccagc gcgaccgcgg cgggaagctt gccgatgccg acggtacgtt
     5221 tcttgaagat ctcgttgccg agcagcaggc cttcgtagtc gcccagccgg tgccgcacga
     5281 ccttggccag acgcgacgac tccttcagga atccgcgcgg cagatcttcc ttcaagccgc
     5341 cgatccggtt gtaggtgaag tgcatccggc cgccggtcgc ggcctcgagc agcccctgga
     5401 tctcctcgcg ctcgcggaac gcgtagaaca tcggcgtcat cgcgccgagc tcgaggccga
     5461 acgatcccat gaacatcagg tggttgagca cccggttcca ttcggccagc agcgtgcgga
     5521 tccactgggc gcgctcggga acctcgatct ccatcagcgc ctcgacgccc agcacgaccc
     5581 ccagctcgtt gccgaaggcg ctcagccagt cgtggcggtt caccagcgcg atgatctggc
     5641 ggtagtcgcg cacctcggcc atcttctcga agccacgatg catgtagccg atgaccggct
     5701 ccgcgtcgat gatcagctcg ccgtcgagag taatgaccat ccggagcacc ccgtgagtgg
     5761 acgggtgctg cggcccgatg ttgagaatca tctcctccgt cgcgaaccgg ctcgatcccg
     5821 cgacgtcgag cgcgatgcct tcgccgggtc cgcccggcag catggtggga tcggacacgg
     5881 ctactccggc tccttcgcgc ccggccactc cttggcctca cgcgtcatca ggacgaactc
     5941 tttgcgcagc ggattcccga cgaagggctc cgccagcagc agctttacca agtgcggatg
     6001 accggcgaag tcgatgccga acatctccca gcactcacgc tcgtggaagg atgcgccgcg
     6061 ccacaggccg gtcagcgtcg gaacagaccc gccctcgcgc ggcaccatcg tcttgaggtt
     6121 cacggcctgc agcgacgagg ccgagtacag atgcgcgatg acttcgaagc cgtcgtcttc
     6181 catgtccacg gccgacagcc agttgaacat cgagaagttg cagcgcgggc accggcgaag
     6241 gtgctcggcg acgacgtgcc agtttgccgg cgtgacgtgg agcgccactt ggccttgcgg
     6301 cgtgacctcc tcgcgctcga cggcgccggt ggcttctttg acgtggtcga tcagtgcttt
     6361 cgcggacatc ggctaccggt tggaaaggtc gacgggtccg acggggccgc cgacgacgat
     6421 cgcttcgcga cgctggcgct cgaagcgttc cttgagcgac tcgtcggcga tctgcgtctg
     6481 cagtcggatg attccttcca gcagggcttc gggccgcggc gggcagccgg gcacgtagac
     6541 atcgacgggc acgatctggt ccacgccctt ggtgaccgag tacgagtccc agaacgggcc
     6601 gccgcagttc gagcacgagc cgaacgagat gcagtacttg ggctcgggca tctggtccca
     6661 cacacgcttg agcgcaggag ccatcttgtc ggtcagcgtg ccggcgacca ccagcaggtc
     6721 ggcctgacgc gggccgtgtg cgacgggaat gacgcccaga cgcatgaagt cgtggcggct
     6781 ggtggatgcg gcgatcagct cgatcgcgca gcaggccagg ccgaagttca tgacccagag
     6841 cgaatatttg cggccccaat tgaagacgaa cttgaccggc tggggcagat tgaccttgtc
     6901 gactacgccc atggctgcga ctcccggatg gcgcggctct cggagggctc gacccagtcg
     6961 agaaccttct tcttgtaggc gtagagcagg cctacggcga ggatcaggat gaagatgatc
     7021 atctcgacga gcagcaagcc cggccggggg aacgtctcga acacgacggc ccaggggaac
     7081 aggaacaccg actcgacgtc gaagatcacg aacaggaacg cgtagatgta gtagcggatc
     7141 tgcgtctggg accagccctc gccgacgggg tcgatgccgc actcgtacgt cgtgtacttg
     7201 tcgttggcgg gcttgcgggg tcgcatcagc cggttcgccg agaaggcgat gacgacgaag
     7261 agaacgccga gaatcgccag cgctccgatc gtgccgtact gccagaaata ctgcgtcatc
     7321 cgggcccccc gcccacggcc ttccacggcc cctgcgttgt catctatttc cgctggtcag
     7381 agccatcatc gagcgttcgt gaaaacgctc acgtgttcga gtccgcggga ttctagcgcg
     7441 gaaggccgcc gatccccaaa gcggaagggc gccctccgct gcggccgatc ccggctcgat
     7501 acgcaacgtt gacataactt tccatctcca cccttgcatt cgcatccgcg aatatcgtaa
     7561 taatctcccc acgggagtgc cggaaggggg gcgggtggcc aagttcgggt tcgcaatcga
     7621 tcaccgcgcg tgcattggct gccacgcgtg cacggtcgcc tgcaagatgg aaaacgacgt
     7681 tcccctgggg gtcttccgaa cctgggtcaa gtacatcgag aagggcaact tccccgaggc
     7741 gaagcggtac ttctcggtcc ttcgctgcaa ccactgcgag gacgcgccct gcaccaacat
     7801 ctgcccgacg tcggcgctgt tcacccgcga cgacggcatc gtggacttcg acaactcgat
     7861 ctgcatcggc tgcaaggcct gcatgcaggc ctgcccctac gacgcgttgt acctggatcc
     7921 cgggacgcac accgcggcaa agtgcaactt ctgcgcgcac cgagtcgagg tgaacctcga
     7981 gcccgcctgc gtgatcgtgt gcccgacgca cgccatcatc gccggcgacc tcgacgatcc
     8041 gatcagcgag atctcgcgct tcgtcgcccg gcaagagacc caggtccggg cacccgagca
     8101 gggcaccaag ccgaaggtgt tctacgcggg ggccgacgag gccgccatcg acccgaccca
     8161 gaccacggtc ccggacgcct acatgtggtc cgaggtcaac ctgctcgacg ggcagctgca
     8221 gccgcggatc gacgaggccg aggcgcgcgc gcgcacggtc tacgacgtgg accaccccat
     8281 gccgtggggc tggaaggtgt cggcatacct ctggaccaag tcgatcggcg ccggcgtggc
     8341 gctgctggcc gcggcggcga tgctgcgcgg ctacgggcag atcccgctgc tcgccacctt
     8401 cgctccggcg atcgcgctcg tgatgacggt gatcaccggc gcgctgctgg tcgccgacct
     8461 gaagcgcccg gagcgcttct ggatgctgat cctgaaggga aatcccactt cgtggctcgt
     8521 gcgcggcgca tgggtgctcg gagcgtactc cgcgctgctg gtggcctggc tcggcggccg
     8581 cgtcctcgat ctgccctggc tcgtcgacgc cgtgcgatgg cccgcgatcc cattgtcggc
     8641 cctcgccgcc gcctacaccg cgtggctgtt cggccaggcc gaaggccgcg acctgtggca
     8701 gagcccgctg ctcgcgccgg tgctgatcgc gcaggcgacg ctcgtcggcg cggcctcgct
     8761 gctgctggcc tcgctgttcg ccggcggcga aagcacgcaa gtgctgatga cgcgggtgct
     8821 ggtcgcgtcc gcggttggat gggcggcact cacggtcgtg gagttctcga gccattcgac
     8881 gcagcaggcc gcgaaggcgg cacgcaacct gatccgcggg ccgtaccgcg tgtggttctg
     8941 gaccggtgtc gcgctgggca tggtgctgcc tgccgcgctg ggcgggctcg cgctggcgac
     9001 cggaagcagc gggccgatgg tggcggcctc gatcgcggcg ctcggcggcc tggttttcta
     9061 cgagcacggg ttcgtcaagg cgggtcagtc ggtgccgctg tcctgacgcg cgaaccccgg
     9121 ccgctaggcg ccggtcagcg agaagtgatg gtagtccttc accttggagt agttgccgcc
     9181 ccagaaccag ccgatctttt cgaaggcccg aaccactgcg tcccccggct tgatcatgcc
     9241 gggacggtcc tgggtgcgat cgacgtaggc cttgccgtcc ggtggggtca cgccggcgct
     9301 cgtcacccga gggttctgca cggggttgat gtccacggcc aggccgtagg cgtgctgaga
     9361 aagggtcgag gatccgaccg cgggacggca attgaacgcg ctggtggcgt tctgctgcgc
     9421 gcgagccggg tcattgccga tgtagccgtt ggcgatcacc atgttcgcga tcggaaaccg
     9481 cgcctcgaag atctcggcga atgcacccag gaccgcctcg gcctggtcct cgtgcacgat
     9541 catctcaccg cgcgcgacgg tgccttcgaa cgtccagtga tccatccgga gcacgcggag
     9601 gtcgtgcagc gggaccgggc agccgggctt ccacgacgtg cccatctttg cgcgcgcgtc
     9661 cgcgtcgatc acctcgacgg ctcctcggaa ggccgggccc gacggcgaag gcgccggcgt
     9721 gggcgaggcg ggcgccggcg gcggggctgc ctggtcggcg gcgccggagc acgcgccgag
     9781 gacggcggcc agcatcagag ggaagaagcg acgcatggaa tcgcggccga gcctaccgac
     9841 cggggctcgg ttccggaaac cgggcccgga aacccttgca ttcacatact cgaatatcgt
     9901 aataatcctg gcaggagcgt ccgagcggga gggggacgag ggtgacggag ccgaccaagc
     9961 tcgaaggcgc cggcgggcgg atggctcaat atccgccgcc ggagcggtgg gacgattggg
    10021 tcgagtacga gggctccgcc tggccgaaga aggtcgaacg ccactacatc ctggtcccga
    10081 ccgtctgctt caactgcgag tcggcctgcg gccagctcgc ctacgtggac cgcgacacgc
    10141 tcgagatccg aaagatcgag ggcaacccgg tccatcccgg ctcgcgagga cgcaactgcg
    10201 ccaaggggcc ggcgacgctc aaccagatcc aggatcccga gcgcatcctc tatccgcagc
    10261 ggcgagccgg tgagcgcggc agcggccggt gggagcgtgt ctcgtgggac accgcgctcg
    10321 acgacatcgc cgagcgcatc gccgacgcca tccgcaacga gcggcgcgac gaggtcgtct
    10381 accacgtcgg ccgccccggc gaggacggct atgccgaccg cgtgctgcgc gcctggggcg
    10441 tggacggtca caacagccac accaacattt gctcgtcgtc tgcgcgcgtc gggtacgcgc
    10501 tgtggatggg ccacgaccgg ccctcgcccg actacgcgaa cgcccgcttc acactgctga
    10561 tctcgtcgca tctggaaaca ggtcattact tcaacccgca cgcgcagcgg atcatcgagg
    10621 gcaagctcgc gggcgcgaag atcgcggtga tggatcctcg cctgtccaac acggcctcgc
    10681 gtgccgacta ctggatgccc accaaacccg gcaccgaggc cgcggtgctg ctggcgatgg
    10741 ccaagatcat cctcgacgaa gagctctacg acgcgccgtt catgcggcgt tggtggaact
    10801 gggacgagta catgcgggcg cagcggcctc aggacgagcc gacgttcgag aacttccttc
    10861 tcgcgctgaa gcaggaatac gcgcagtaca cgcccgagta cgccgcatcc ctcgcgggcg
    10921 tttccgcgga catgatcgtc aaggtcgcgc gcgaggtcgg ccgcgccggc tcgggcttcg
    10981 cggcgcacaa ctggcgcgcg gccgcggccg gcaacctcgg cggatggcag gttgcccgct
    11041 gcctgttctt cctcaacgcg ctgaccggct ccgtgggcac gaaaggcggc acctcgccga
    11101 actggtggga caagttcgtt ccgcagccgt tcctggcccc gccgccgcag aaggtctgga
    11161 acgagatcct gtggccgcgc gagtacccgc tcgcgcacca cgagatgtcg atcctgctcc
    11221 cgtacttctt gaaagagcgg cggcagaagc tgtcggtgta cttcacgcgc gtctacaacc
    11281 cggtctggac gaacccggac ggattctcgt ggatcgaggc gttgtcggac gagtcgatga
    11341 tcggccttca cgcggccctg acgccgacgt ggagcgagac ggcctggttc gccgactacg
    11401 tgcttccgat gggcctgggc cccgagcggc acgacacgca ttcgtacgag acccactcgg
    11461 gcaagtggct cggattccgg cagcccgtgc ttcgcgtagc cctggagcgc atgggcaagc
    11521 cggtccagta cacctacgag gcgaatccgg gtgaggtctg ggaagaagac gagttctgga
    11581 tcgagctttc gtggcgcatc gatcccgacg gctcgctcgg cgtccgccag tggttcgagt
    11641 cgccctaccg gcccggcgag aaggtcacga tcgacgagta ctaccagtac atgttcgagc
    11701 acaacgtgcc cggcttgccg gagaaggcgc aggccgaaga cctcacgccg ctcgaataca
    11761 tgcgcaagta cggcgtcgtc gagatcgcgc cgaccgttta cgaggtccac gagaatccgg
    11821 tgcccgagag cgcgctcgaa ggcacgaccg tcgatccggt caccggcctc gcgaccaagc
    11881 cgggtgagcc gccctccgga aagccgatcc cgctcatcgg gccgtggaac gcgccgggcg
    11941 tcgagatcga cggcgtggtc cgcgagggtt tcctcacccc gtcccgcaag ctcgagttct
    12001 acagcccgac catggcggag tggggatggc cggagcaggc gctgccgaac tacatggaaa
    12061 gccacgtcgc gagcaccgac ccgaagggag gcacctacat cctgatgccg accttccggc
    12121 tgccgacgct gatccacacg cgctcgaaca actccaagtg gctcaacgag atcagtcacc
    12181 ggcatccgct gtggatccat cccgaggacg ccgagcgggt cggcgtgcgc gacgaggacc
    12241 tggtgaaggt catcacacgc atcggtcatt tcgtgatccg cgcgtgggtg accgaaggaa
    12301 tccggcccgg cgtggtcgcc gcgtcccatc acatgggccg ctggcggctc gaccgcgggt
    12361 tcggcaccga ccgctggtcc tcggcgctgg tggatctcac ctacgaaaac gcctcgacgg
    12421 aaggcgcaac ctggcgcatc cgccaaaagg agcacgtgca gccgttcgag agcaaggaca
    12481 aggactcgtc gcgcgtgtgg tggaccgatg cgggcgtgca tcagaacctg acgttcccga
    12541 cgcagccgga tccggtgtcc gggatgcatt gctggcatca ggtggtacga ctggaagccg
    12601 gcggacccga cgatcagtac ggcgacatcg tcgtggacac gagcaagagc ttcgagatct
    12661 acaaggagtg gctcgcgatg actcgtcccg cgccggggcc gggaggacta cgccggccgc
    12721 tctggatgtt ccgtccgctc aagccggacg cgagcaccta caagatcccc gactgaggtt
    12781 cgagcaggaa ctagatgaag aactgaaccg acgactcttt catctcgagc agcgcgctgg
    12841 cggcaccgag cggcggctcg aggccgtcga tgaggtcttc gcgcttgagc ccgagcatgt
    12901 ccatcgtcat ctggcacggc atgagcttga cgcccatgtc ctgggcatac tccatgagct
    12961 cccacggcgc cgcgaccttg tagtccttgg cgagcttgag catcatcttc ttgccggctc
    13021 cgccgaagtg aagcttcgac agcttcgcgc gcttcatacc cggcgggttc accagagaga
    13081 agcccttctg catccagttc gacccggtga tccggacgcc gggcttcgcg agcgcgaaca
    13141 gtccccagaa cgtgaagaag atggagacct gcatgccgct cgccgaggcg gtcgtcgcga
    13201 ggatcatggt gggccagagc ttgtcgagct cgccggaaaa ggcaacgatc gaaagcttgt
    13261 tgttgtcctc ggccatcgga cacctccatg gacccggatg ccgccgggcc ccgctggtgg
    13321 acccctactg gggtcccggt tcggcccgct ccctgctggt ttcgggccga ctttcccccg
    13381 ttactcagcg ggattataat atgcacaagc gcgcatttac aggggtgggg gcgcgatttt
    13441 tcggcctatc ccggcgtgtg gttccctcgc ggaggggaga tactccctcc gatgggggag
    13501 gaggttgatg agcgatggac gtatccgtac ggaacgagat ctacgaactg cacgctcgcc
    13561 tgtgcaaggc gctggcggac ccgaagcggc tcctcattat caacgcactc cgcgacggac
    13621 agaaatcggt gggagagctg actgcggagc tggagctctc gcagtcgaac gtgtcgcagc
    13681 atctggccgt gcttcgcgag cgtggggtgg tcgcggcgac cagggacggc aacaacgtct
    13741 tctatacgct caaggacgtc cgggtcgtcg aagcgctgga tctgcttcgc tcggtcatgg
    13801 ccagccagct ggaccggcgc agcgagctgc ggccgatcgc aacgggctga gcggctagtc
    13861 cgcgcagcgg aagcagcgga tctcgagttc gtcgatccgc tgcttgtagg cggcgaacgc
    13921 gtcgctccat tcctgaagcg tgcccttttc ctcgtccagg ctgttcggcc ttaggcggac
    13981 gagccagccc tcaccgtagg ggtcgcggtt ggcgatgagg atgtcccgct cgaaggtctc
    14041 ggtgttgacg gccacgatct cgccgctgag cggcgatgcg accgggccca cccacttcgc
    14101 cgactcgatg gtcgccaagc cccgtccgcg cgagatggtc tttccgacgc ggcgaaagtc
    14161 cacggcgacg agcttgccgc accgtgtttg tgcggggtcg gtcatgccga tcaccgcctc
    14221 gtctccctcg agacgaatcc acacgtcggc ttcgacgtcg tagagaaggt cgcgcggaat
    14281 gacgcagccg tagtggatct cttcgggagc aggttcaagc acgcgacgtc accctccgca
    14341 ctcgatgcct tcctcttgca tgaacgcctc gtacgcgtcc agcgcggcct gtccggtcag
    14401 caggtccgcg atatcgcggt cccaatcctc gggcttgatc tttgcgaccc atccgtcgcc
    14461 gtagggatcg cgattgaaga tggtcggatc ggactgtccg gcctcgttga tctcgacgat
    14521 ctcgccggcg accggtgcag gaaccggccc gacccacttg ccgctctcga gcgtcgcggt
    14581 gctcttgccc ttgacgacgg acttgccgac cttcttgggc gtgaacgaga tgaacgactt
    14641 cgcgaggttc tgcgccacgt cggtgatccc gcagaccacg agatcgccgt cacggcgagc
    14701 ccacacgtgg ttcttcacga ggtacagcag gtcgtcggga atattgcagt tccgcactgc
    14761 ggtcacggtg tcccctcccc cgtgggctcg gcttcgagcc cggcttcggc aagcaagtgc
    14821 tccgcttgga tgtgatagag cagcatgtcg aacgcaacca gcttgatctc ttcctcgaac
    14881 ttctcgatga accgaggatc gcgaagccgc ggcttgatct cacgcatcca cggcgcgtca
    14941 cagaccgggc agagcacttc gtagaagcgg cggccggact tgtcgtccac gcgggtcgtg
    15001 accttctccg ggtgctcgtc ggccaggtgc cggtgaagcg cgctgcgcgc tccctcgaag
    15061 tcacagtacg ggcagctcat gattgctccc gttctcgccg cgcgccttca gcgcggtctt
    15121 cgccagacgc ccagcgacat cgcgcaccag cgccaccgtt tgcattcctg ccgcggtcgc
    15181 gtacacgcgt ccggtgtcca gggccttgga gaccagcccg gtttgaagca gatcaccgac
    15241 ccgctcggac acgaccagca ccggcagcga cagcgtcgcg gccagatcgg cggcggcgat
    15301 gccgtcgtcg gccgacgccg cctcgaggat ccggaagttc gtgcggtcac acgcggcggc
    15361 gagcgcgcgc tgcacgaagt cggtcgccac cgtggcggcc tcctgctcgg tgaggatgac
    15421 cttgggaagc tcgccggtgt cgcccggacc gaagacgaag gtgtccacgc gctcggcgac
    15481 caggatcacg tcccgcagcc gtttccagag gccgtcggcg agcgcgcccg cgtcgtagtc
    15541 ggtcattcgg cacctcccgc cggaacctcc gcgacgtcga tgacgtcgac ggttccggcg
    15601 ccttccacca cgccgaccaa tgcgggctcc ggcgcgccga cgacggcacg ctccaccagc
    15661 tcgacgatgt cgagcacctg aagcttgtcc tcgttccccg tcgtcttgac cgcgtcgcgg
    15721 tacatcgtga cgtccttcgg gcacgcgacg atgaagtagt ccacgcccag cgccacggcc
    15781 tccttgatgc ggttctcgct cggccgttcc ttctggttgg agtcgtccat ccagatgcgg
    15841 ccgccgcccg cgccgcagca gaaggtgttc tccttgttgc gcggcatctc gaccagttcg
    15901 attccgagca tctcgaggat cttgcgggga gcgttcgtca cgccgttgta gcggcccagg
    15961 tagcacgggt cgtgataggt cacgcgcatg ctcagaccct gcttcggcac gatccggccc
    16021 tgctcgagca gccggtggag aagatgcgtg tagtgcagga cgcgccagtc gccgccgaac
    16081 tcggggtatt cgttcttgag cgtattgaac gagtgcggat cggtcgtgac gatctcgttg
    16141 aagtcgcagc cgttaatgac cttgacgttg tgctcgacga gctcttcgaa caggccttcc
    16201 tcgccgacgc gccggacgtc gttgccggcg ttgcgctcgc cgtcatagag gatgccgaac
    16261 gagatgccgg cgcgctgaag gagccgggca aacgtccgcg acaggtcctg gagccgttcg
    16321 tcgtatgagg cgtagtcgcc gacgaaccac agcacctgga cgaagcgctt gcgtgcgtcg
    16381 tggatctcga agtcgagctc cttggtccat ttcgcgcgca tcttcggcga cttgccgaag
    16441 gagttgccgt tcttggcgat gttctgcagc gcgtcctgga gcgtcgggtc catcgtgcct
    16501 tcctcgacca aggagcggcg catgccgacg atggtgggga cgtgctcgat gccgacggga
    16561 cagacctcga cgcaggccat gcacgtcgtg caggcccaga gcgtctcggc cgggatgatt
    16621 ccaccggcga cgggcggcgg accgtcgccg gtgtatccgt tcgcggcacg tcgttcccag
    16681 tccatgatcg gccggatccg gttcgactgc tcggcgaact gacgaagatc gaggatcaag
    16741 tctcgcggag agagcggcgc gccggccgtg cgggccgggc agacctcgtg gcagcgaccg
    16801 cacttggtgc aggcgtcgaa gtcgagcaac tccttccagg tgaagtcgct gatcgacttg
    16861 atcccggcag acgcgcccgc agggacgcgc ggcagtgtca gcgtcgactg ccgctccgcg
    16921 aaggtgaggt tggctgcgtc ggtcagcatg tgcatggcct tcgagaacgg gatgtagccg
    16981 atgaagatca gcgccatcgc ggcgtgcgcc caccaggtgt acaggtgcgc gtccgtggcc
    17041 gtggcggccg acatgcccag gccgcgaatc gcgcgcgccg tcaaccatcc gaccggcgac
    17101 cagacctcgt aggccgggaa gttgttggca tggatgcgaa ggccctccag caagaagccg
    17161 ccgacgccca gccagaacaa gaagtgccag aacagccagt caccccgctt gtacggcttg
    17221 cgcgagtagt cggccgagtc cttgtccacg cgcgtgtagt cgagtgcgaa cggcttgacg
    17281 aagcggcggc gatacgcgat gtaca
//
LOCUS       DATLFO010000200        19498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_74014, whole genome shotgun sequence.
ACCESSION   DATLFO010000200 DATLFO010000000
VERSION     DATLFO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74014"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VM841_08255"
     CDS             <1..762
                     /locus_tag="VM841_08255"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVL90210.1"
                     /translation="SLGSRTAALSKAYADEAGHTGFLYRDAKALGDFFEAATRAGLQA
                     GVHCIGDAGCEAAVAGLERAAERAGVEAVRAMRHRLEHFEMATPAMIARAAELGAVFS
                     VQPAFDTFWGGAEGMYAARLGVKRAAPMNNIKAMLAAGTTVGFGSDCPVTPFDPLAAI
                     RAATEPSNPAHAISQEHAFHAATLGAASMAGESIPAGMIAPGHRADFVVWEADPVRSR
                     RPAIRATVARGSAVYGMLGDRKSERGAKPRTSLAP"
     gene            complement(867..1028)
                     /locus_tag="VM841_08260"
     CDS             complement(867..1028)
                     /locus_tag="VM841_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90211.1"
                     /translation="MIVRSTLDEVWEVAFLREDGIGLQLVHSKDLAGRLAQTFEALDA
                     VVLAVTRIA"
     gene            complement(1310..1765)
                     /locus_tag="VM841_08265"
     CDS             complement(1310..1765)
                     /locus_tag="VM841_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HVL90212.1"
                     /translation="MAYWLMKTEPGTFGIEDLARKKREHWDGVRNYQARNNMMAMKAG
                     DGVLFYHSNAKPSGVAGLAEVVREAYPDHTGQDPSSKYFDPKASPENPIWMMVDVGYV
                     ETFPRVVSLAELKQTPGLEAMAVLRKGSRLSVTPVTQEEFEIICDLGRP"
     gene            1877..3679
                     /locus_tag="VM841_08270"
     CDS             1877..3679
                     /locus_tag="VM841_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL90213.1"
                     /translation="MFRRARRVLPLVLLAAAMLVPVQGRAQIPGLEYILVSDGTPIAV
                     NLQFPSGYQIGQRLPILIQIDGYGGASSPMNPNSATFGNGEYVTAHMSLRGTGCSGGQ
                     FDLFDRRSSYDGYQVIENLAKRDWSNGKVAIWGHSYSGLTGWLTAATQPPSLKAISVS
                     GLIDDLYRGIVYMGGVSNMGFPLLWTGIYRPSSEYQNGVIPGVRSGDRTCIANQATRY
                     PPDVLDNPILNGAAGVEDSQWWASHSTITYLDGINVPTHIVQSYQDEQTGPRGSNLLW
                     QRLDERKPELPKRLLLTNGVHSTNTAPPEIRDDRIAFLDCHVRGVCSADILDPSQRVK
                     VLWEMHNDGTRLRSTGVTADSDWPLRQTQWTRYYLQPNGGLSTSMPAAADTAPYVSGS
                     KRAGAWSYLAGANQGEQLTTANLPDEARFTTVPFTTATAFAGPINMTLFATSTAPDTE
                     FYVELNDVDAAGNVTRLQRGMLKASHRDLDPLRSDYNGDGEIIRPYHPHTNTTTKVLV
                     PGSVYQYEIEVFPVGHVFRPGHALLIRVTQPPANDSLATYVPTTPPGVNTVLIGGDFA
                     SSILLPLVPAATVGGPDLGCGKQVGLERCSKPVL"
     gene            complement(3894..4088)
                     /locus_tag="VM841_08275"
     CDS             complement(3894..4088)
                     /locus_tag="VM841_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90214.1"
                     /translation="MTEGEQALCDWCEEPRQPAIDRMDVSNGADDVILHLDLCAEHAS
                     HAESRLIRAERLQRIQASLS"
     gene            4385..6079
                     /locus_tag="VM841_08280"
     CDS             4385..6079
                     /locus_tag="VM841_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90215.1"
                     /translation="MSDSAQKPVGRVIGTDPATPLEFWVAVGEGEFLQLDDVIACERD
                     VPGPGPVSIYGIVSQVRARHEGAKFDSDVFLIEDGFLPAEIAEAAQITTTRFEPEVFV
                     PPLPGAEVRRASGKERDQALFFDGMDKRLAAGMSREGEPIFANLEFLDGTRGAHVNIS
                     GISGVATKTTYATFLLYSLFNSKALGAEAINTKALVFNVKGEDLLFLDHPNAMLDGEQ
                     SDRYRRLDLPVGPFPSVGVFAPPRRGSAAAVPDVASRIEGVTSFFWTLAEFCAEELLP
                     FLFADAEDDRQQYTLVVQNVAQRLKHDAAPLPEGGIEIEGVFIRSFRELVDFLIDRVQ
                     DEDHMPYWAGRAIGAGTVGAFVRRLQASVRHIEHLVRGDVPYADKHKVALDKQVTVVD
                     LHNLNDRAKRFVVGVVVRRAFEAKERTGAAKPLLFLVLDELNKYAPREGSSPIKEMLL
                     DVAERGRSLGIILIGAQQTASEVERRIVANSAVRVVGRLDSAEAGRDEYGFLPKAMRQ
                     RATILKPGSMIISQPQIPIPLLLEFPFPAWATRSSEAGANPAGSDHPDDPLDGLPL"
     gene            6076..7233
                     /locus_tag="VM841_08285"
     CDS             6076..7233
                     /locus_tag="VM841_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HVL90216.1"
                     /translation="MKILHTSDWHVGKTLRGRSRIDEHEAVLAEVASIAEREQADLVL
                     VCGDLFDSAAPAPDAERAVYRALLALTDTGATVAVLAGNHDSDRRLHAVKPLLELGRI
                     VVGMPSENPVTLVQGRGGDTASLVMLPWVGKQHVVKVEELMKMDGAETQQHYAERVKR
                     IVHHLCAPMSADTVNVLAAHMTIAGAETGEEVRSAHVFDYAVPGTVFPASLHYIGLGH
                     FHTPQKVAAPSLAWYSGSPLRLGFGSDAEERADKGVVIVEATPGAPAVARLEPLTSGR
                     RLRTIRGTLDQLENMARNDMTGDDYLRVFVREQAKAGIAQQVREWFEHAVDVQIDPQD
                     EGETAASESTRAGRSPGELFRDYLAHKQANDERVIALFDQILEEMHAADTA"
     gene            7214..9595
                     /locus_tag="VM841_08290"
     CDS             7214..9595
                     /locus_tag="VM841_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HVL90217.1"
                     /translation="MRPTRLELEGFTAFKQPTVVDFDGCDFFALCGQTGAGKSSIIDA
                     ISFALYGSIPRLDRNDVRSVISQGLLECKVRLDFTIGETAYTAVRVVARTKIGATTRE
                     ARLQRGAETLAGDAKAVSAAVEGLIGLTFEQFTKCVVLPQGEFARFLHDKPADRQDLL
                     VKLLGYELYERMGRRANELARRAGDQAEWLAKRLEDEFIFATPESRTEAAERLATLDA
                     VRVRVEDATPGIEALDADAKEAKAEAAAARARLDRLAKVSVPDGAGALAGAVARARDA
                     REAAEAEAERTDAARRAAEAALEALPQRSVLEIARQAHADRAKCETQRTQVMAALEAL
                     GRVAGEAAVAFDAAVAAMEAAERALEAARIQHRAHDLARSLVAGDPCPVCKQTVSTLP
                     GDDVPADLHDAERALEQARAAREDADRNRRKAADDKTRAEVTADELTATLDELTRRVA
                     DHPDPDQLDRALTELESAEREAADARRAGDAARRAATDAQRRLDGAMRAEQDARKGFD
                     QARDALADLSPPPASRDDLAADWEELAAWAATQHAAQAAAADAADRRVEEILEQRRAR
                     AGELLAAARDAGIEVPHGVKLSEACMQAVVSQRAVLERIEAALIQMEDVAAQRKTAEE
                     EQAVASTLGRHLSASNFEKWLLDEAMQRLVESASRVLRELSAGGYSLAVDGKGNFAVI
                     DHRNADEQRSAKTLSGGETFLASLSLALALSEDLAGMAAEGARLDAIFLDEGFGTLDP
                     ETLGVVQSAIESLASQGRMVGLVTHVREIADYVPVRFEVRKHPDTSRVEKVDA"
     gene            9592..10551
                     /locus_tag="VM841_08295"
     CDS             9592..10551
                     /locus_tag="VM841_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90218.1"
                     /translation="MRFTVEPWSPEYAAPFDSDAAPEPGAPPAAEVDERVEAEPAEWT
                     PKPPPHGAVPAKRVMFVDGVQRIDAWVWVAAEDGTVRPGICASYAAGVVCCDGRADVP
                     DIEVRRGLFSAAPVEPINARRVVYRPYGATGESVDQLRHAVQQRMGELEADLARRCGP
                     ADLIVLDGPLRGRQNVPDAIGFVKSHQQRYLSDPAHAVVGRLGAGERTPLFLMTTSWT
                     RYSWYLRLPGPAAQPWSSVVRCEASADLSAAEATRVADRAAATLPMFASAAHKDPRAP
                     QNLYPIGGLERALRHRLGDPALLYRELRQAASRPRSAAAAPAG"
     gene            10710..10967
                     /locus_tag="VM841_08300"
     CDS             10710..10967
                     /locus_tag="VM841_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HVL90219.1"
                     /translation="MVDDEEETEAPDASLDEILAKRAEEKGEDEEEESVIELTREERA
                     ESLAIKVLPQQADEFTCRNCFLVKHRSQLADRKKIICKDCA"
     gene            11089..12798
                     /gene="lnt"
                     /locus_tag="VM841_08305"
     CDS             11089..12798
                     /gene="lnt"
                     /locus_tag="VM841_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVL90220.1"
                     /translation="MASGVVATFAFPPAQLRFVAFAALVPFLLSLRQATVRDGAVAGA
                     AFGLAFMGVLIGWISIVGWIAWAALVFAQMLFYAGFGALAALLGRAGPVARVLGWPLV
                     FTGFELARSRFPLGGFEWGVLGSSQEAVSTLTPLARAGGVFAISIALVAINALLSEAI
                     AARRKRNAVLAVALAAAGAIAPAMMPLGAVANSGTIDVAMVQGNVPRGYFAGLRRGRV
                     GPEDEIIIANHLRLTDTLAANPPELVIWPENALDRDPFTDQEARRAIVDATAATGAPL
                     LVGAIIDGPRPGTFFNANLLFDEGGRLLGRYDKMKLVPFGEYVPWQWTRSVVPALSAI
                     GSDGIPGDEPVVLNANGTQIGAVICFESSRAELVRRFVERGAEMIVVSTNNATFERTP
                     LARQHLALSRMRAVETGRPVLHAAISGISAIIQPDGSIVQQAGLFRPAILRQRIQTTS
                     GLTPYVAYGATIELAYGIGAAVAAIVALLFIRRRPSTVAEDDEPDFWLAPVTAAHDEP
                     HPLLAHVDDADHSSAPPPGPSPAPSSSPASVATKDPAPPARDEPEAEAEPPRGDRGAE
                     AAQ"
     gene            12795..13544
                     /locus_tag="VM841_08310"
     CDS             12795..13544
                     /locus_tag="VM841_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HVL90221.1"
                     /translation="MNALLVLPTYNEAANIREVVERALAATPDVDILVVDDNSPDGTG
                     RIADEIAAAQPRVRVMHRPGKGGLGPAYIAGFTDGLARGYEAFIEMDSDLSHDPADLA
                     RFVAAVKDADLVIGSRYIPGGGVTNWSKNRVRLSQAGNLYARTLLGFGLTDSTSGYRC
                     YRRGLIETLPLRQIGSEGYTFQIEMAWRSWTLGFIVTEIPIVFRERTEGSSKMSRRIV
                     FEALWRVLVFALRFKRAPRRAHPKSVATSPG"
     gene            complement(13614..15752)
                     /locus_tag="VM841_08315"
     CDS             complement(13614..15752)
                     /locus_tag="VM841_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90222.1"
                     /translation="MLLTTRTISRWLIAPLALAAALATGAPTSSTLDQILTATSGRVD
                     LSATPAAMPAALAPLAGGQKVSAAQVNAAQSAASLSGSGTRGRLVVPIGDANGDNRPD
                     LIDFRATNGTLSYRLLSSHNASTIWQAAPIVRPSTIHVAVYPAQINGGAMDVYLLEQH
                     EADRELRWTLHAVDGATGNDIWATGGSLPTDTPDTTPPTVPPSSPPSIPSVSPSAIPS
                     VIPSGVPAVPSIPPSGLPSVLPSDVPTTPPSGLPPLPGLPSEVPSELPSEVPSELPSG
                     IPSEIPSDLPTTVPSGLPTPTISPPTESARQRAASQDSPVPFAAYVTGSAPADVDGDG
                     SIDFALTQTVAFHHAGTTAVVSTLTTVAAASGAPLASVTAVGRDAVPAVAVGGDFLGT
                     GSRGLLAVDEIATGNGNLVTYRAFTASGVPLFAASDSVAPGYVGVFDARHDLNGDGLA
                     DILINRYPSVPGTGPTQVAVRTAPLGQSLWSRTLDEAAIAFVGGSFDAAAGLDVQTQG
                     LHVSAPGRIPSKVVYRALGGANGTSLYSRDVPVANGGGIGDWSAKLSPSAGRIDADVH
                     PDMVHAVNAVPSDGLATQWSQAAVSAQTGALLWSRTSTLDPGAPVGGNVVGDAAADIV
                     DVQESVTRTSDSLVIKTASGANGAPAWTSRVLVGGGTERVRDVHTQSMNPNGTGDIVI
                     TVLQRSGTRERTLIVGVRGATGQVLWIRTT"
     gene            15947..16504
                     /locus_tag="VM841_08320"
     CDS             15947..16504
                     /locus_tag="VM841_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90223.1"
                     /translation="MGGFRASRLGLVLLAAGTGTAAHVAGYFAVFHDAHGRAEALHRS
                     GHDYLAFLAVPLVLAAAGGLALSLRRGLHRTARPVAQAEAFRFLLGVQVAGYLGSEIL
                     ERGLTGELLAPETLVVWAAGAALQVVFAAAGALLYVAAERVGVAVASARRVRGRDSHV
                     RSRPRSSRPRRIAPLIRLAPRAPPA"
     gene            16751..16969
                     /locus_tag="VM841_08325"
     CDS             16751..16969
                     /locus_tag="VM841_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90224.1"
                     /translation="MKKLSAVMIALVVSLAGSAALAGHGGKTYCEENNKRNGMLTKDV
                     HEMHDRVKPISPELAEIIHDEVEPRTCE"
     gene            17023..>19498
                     /locus_tag="VM841_08330"
     CDS             17023..>19498
                     /locus_tag="VM841_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90225.1"
                     /translation="MLVVASIAATPGFADSGCAAVHRYPVSGAEWIVRDVPEFPHGDQ
                     ALRAHAVDPERPDRIWGTNGHSVMRTVDGGCEWREVLRAGPGSEIVRLAASADGHTLL
                     AMQEVGASEVAIAAASGVSIAVSRDGGERWSDFTALAGNGRGGPVAIASSHPGIAYAA
                     VGSVLWRWGEGDWQPAGPVAEPPKTPDLEPVIRRIEIDPFDPNRLYVKGSYSLFVSHD
                     AGESWRRFDRSGDPAGSGLFGPLLARPSASMPASMLLMRGAYPTSPIQEYFESRDGGE
                     TFARYDTSRFGGFIGGVPVAGAARAPDDVLVATDQPGGGAAHGVWRLDPATRRFVDVD
                     RYGFGPFNQVHALAGSGSGYSLSSPSTIATYRDRGRPPSFVARELPPLVHIPPFDPPP
                     PPPVPPATLDGIPSRVALEAAATASFGVGVFVPPAPTPIDVFFLVDTSGSQTPSVRGL
                     ATGIDDIVRALAEAGIDAQFGLGEFRDVKTLRYRRRADIGPPDRRFQYQLERLHTAGG
                     VEEPHLTALHQVATGSGLPAVGDSLAVPAGQDATWRRGSLRTVILISDEPFGTDPNGP
                     SRDKTIAALRADGARVIGVEIDPPAEAAVFDAAGRTFASTLRRQMEDVARHTGAFAPA
                     GGVDCTGNGTFEVEEGAPMVCSIPVDEAAGVARLADPLVRILSQLADMQTVRLAAMPP
                     DGVTATVDPATGIAGVDVKSASRHAFTVTVGCHPGRSVAAPLRIEAVVAERVRATQHT
                     LVECGAAPPAPVLPGPGPAAALVPPAAAQVQPASPNTPNYMANPGAYGQIQVNPAPRD
                     EHEVQENRQHEFTAVRRSRDPASGLYA"
BASE COUNT         2875 a         6947 c         6756 g         2920 t
ORIGIN      
        1 tcgctgggct cccgcaccgc ggcgctgtcc aaggcctatg ccgacgaggc cggccacacg
       61 ggattcctgt atcgcgacgc aaaggcgctc ggcgatttct tcgaggccgc gacgcgcgcg
      121 ggtctccagg cgggcgtgca ctgcatcggc gacgccggat gtgaggccgc ggtcgccggg
      181 ctcgaacgcg ccgccgagcg cgcgggcgtc gaggccgtgc gcgcgatgcg ccaccggctc
      241 gagcacttcg agatggccac gcccgcgatg atcgcccgcg ccgccgagct gggcgcggtc
      301 tttagcgtgc agcccgcctt cgacacgttt tggggcggcg ccgaaggcat gtatgcggcg
      361 cgtctcggcg tgaagcgtgc cgcgcccatg aacaacatca aggccatgct cgcggccggc
      421 accaccgtgg gcttcggctc cgactgtccg gtgacgccgt tcgatccgct cgcggccata
      481 cgcgccgcga ccgagccgtc caaccccgcc cacgcgatct cccaggagca cgcgtttcac
      541 gcggccacgc tcggcgccgc gtcgatggcc ggcgaatcga tccctgcggg catgatcgcc
      601 cccggccacc gggccgactt cgtggtgtgg gaggccgacc cggtccgcag ccggaggccg
      661 gcgattcgtg cgaccgtggc acggggatcg gcggtctacg gcatgctcgg cgatcgcaag
      721 agcgaacgcg gtgccaagcc ccgcacttct ttggcgccct gataaccgca gaggttgccg
      781 gatgaccaaa gaatgccggc ggccaccccg gctgcagggc gttgagagtc ggagccggtc
      841 cgccgcgagc aggattcggc gagcggctag gcgatgcggg taacggccag gacgaccgcg
      901 tccagcgcct cgaacgtctg cgccaggcga cccgcgagat ctttgctgtg aacgagctgg
      961 agtccgatcc cgtcctcacg caggaacgcg acttcccaga cctcgtccag tgtcgagcgg
     1021 acgatcatgg ccgctgcctc cgatgcccac atgcttccgg tccggccgcc gtacccctac
     1081 gagcggcctc accggcttta acgattcgac ggacccggaa gttcccgagt tccccgaatc
     1141 tgtggataac ttaccgcggg gggccgactt tgaacacggg ggaggagggt tccgtgctct
     1201 cgggactcgt caactttctt gacgcgcgtc aactttcttg acgagtgcct ccgaaggatc
     1261 gagcgagacc tgcgcgtgcg ccgtggaaga gggtggaccg cggcccggcc tatgggcggc
     1321 ccaaatcgca gatgatctcg aattcttcct gcgtaacggg tgtgaccgac aggcgggagc
     1381 ccttccggag cacggccatg gcctccagcc ccggcgtctg cttcagctcg gccagggaca
     1441 cgacccgcgg aaacgtctcg acgtagccca cgtccaccat catccagatc gggttttcgg
     1501 gcgaagcctt cgggtcgaag tacttcgacg aggggtcttg gcccgtgtga tccggatagg
     1561 cctcgcgcac cacctcggcc agcccggcca cgcccgatgg cttcgcgttc gagtggtaga
     1621 agagcacgcc gtccccggcc ttcatggcca tcatgttgtt gcgggcctgg tagttgcgga
     1681 cgccgtccca atgctcgcgc ttcttgcgcg cgaggtcctc gatcccgaac gtgccgggct
     1741 cggtcttcat cagccagtac gccatgacag cggcgcagca taccgggccc ggccgggcag
     1801 gagaccgccg ccgttgcggc gaaaccgtcc catatccggt caatgcggga ccgtgcaggt
     1861 gagggaggag tctttcatgt tcagacgtgc tcgccgggtg ctgccgctcg ttctgctggc
     1921 ggccgcgatg ctggtgccgg tccagggccg cgcccagatc cccggcctcg aatacattct
     1981 ggtctccgac ggaacaccga tcgcggtgaa cctgcagttt ccgtcgggct accagatcgg
     2041 gcagcgcttg ccgatcctga tccagatcga cggctacggc ggcgcctcca gccccatgaa
     2101 ccccaacagc gccacctttg gcaacggcga gtacgtgacg gcgcacatga gcctgcgtgg
     2161 caccggttgc tcgggcggac agttcgacct gttcgaccgg cgaagctcct acgacggcta
     2221 ccaggtcatc gagaacctcg ccaagcgcga ctggtccaac ggcaaggtgg cgatatgggg
     2281 tcactcctat tcgggcttga ccggatggct aaccgccgcg acccagccgc cgtcgctgaa
     2341 ggcgatcagc gtctcgggcc tcatcgacga cctataccgc ggcatcgtct acatgggcgg
     2401 cgtgtcgaac atgggcttcc cgctgctgtg gaccggcatc taccgtccgt cgtcggagta
     2461 tcagaacggc gtgatccccg gtgtcaggtc gggcgaccgg acctgcatcg ccaaccaggc
     2521 gacccgctac ccgccggacg tgctcgacaa cccgatcctc aacggagccg ccggcgtcga
     2581 ggacagccag tggtgggcgt cgcactcgac catcacgtac ctcgacggca tcaacgtgcc
     2641 gacgcacatc gtgcagagct atcaggacga gcagaccggt ccccgcgggt ccaaccttct
     2701 gtggcagcgg ctcgacgagc gcaagccgga gcttcccaag cgcctgctgc tcaccaacgg
     2761 cgtgcactcg accaacaccg cgccgcccga gatccgcgac gatcgcatcg cgttcctcga
     2821 ctgccacgtg cgaggcgtgt gcagcgcgga cattctggat ccgtcgcagc gcgtcaaggt
     2881 gctgtgggag atgcacaacg acggcacccg cttgcgttcg accggcgtga cggccgactc
     2941 cgactggccg ctgcgccaga cgcagtggac gcgctactac ctccagccga acggcggcct
     3001 gagcacgtcg atgcccgccg ccgcggacac cgcgccctac gtctcgggct ctaagcgcgc
     3061 cggagcgtgg agctatctag ccggcgcgaa ccaaggcgag cagctgacca ccgccaacct
     3121 gcccgacgag gcgcgcttca ccacggttcc gttcaccacc gccaccgcgt tcgccgggcc
     3181 gattaacatg acgctcttcg cgacctcgac cgccccggat accgagttct acgtcgaact
     3241 caacgacgtg gacgcggccg gaaacgtgac gcgcttgcag cgcggcatgc tgaaggcatc
     3301 tcaccgcgac ctcgacccgt tgcgcagcga ctacaacggc gacggcgaga tcatccgtcc
     3361 gtaccacccg cacaccaaca ccacgaccaa ggtgctcgtg cccggatcgg tctaccagta
     3421 cgagatcgag gtgttcccgg tcgggcacgt gttccgtccc gggcacgcgc tgctcatccg
     3481 ggtgacgcag ccgccggcca acgattcgct ggcgacgtac gtgccgacca cgccaccggg
     3541 cgtgaacacg gtgctgatcg gcggcgactt cgcctcgtcg atcctgctgc cgctcgtgcc
     3601 ggccgcgacc gtcggcggcc cggatctcgg atgcggaaag caggtcggat tggagcgctg
     3661 ctccaagccg gttctgtagg aggttgttcg ggggagggcc ggcacgcgaa agcgccggcc
     3721 ctctttcatt cgccgcggca cggctggcat gatcttgcgt gagcccacac cgacgcgatc
     3781 acgaagccgc accaacggcc agtcaagatc cgcttctgga gcgctgccgc gccctataga
     3841 cgaaggcggc gcctgcatga ggcgccgcct tcgatacggt cgtgtccctt acgtcacgac
     3901 aagctggcct ggattctttg gagtcgttcg gctcggatga gccggctttc cgcgtgagat
     3961 gcatgctcgg cgcacagatc cagatgaagg atcacgtcgt cggcgccgtt ggagacgtcc
     4021 attcggtcga tggccggctg gcgcggctct tcgcaccagt cgcacagtgc ctgttcgcct
     4081 tccgtcacgc tacgccctcc ttgatcgggg accgtgccga ctgacagaaa gactaccccc
     4141 tgtggggggc gattcggaag ggcgtggagc agaaaaaatt ccccgtctag aaaccgggcg
     4201 tccgcttgcg gataatgcgt tccgtcttcc cccgaaattg tcggagccct gggttagggt
     4261 gcttcgacgt gagagcgaat tgggggatca tgggtcgtgc gtttcagctg aaacgcaaag
     4321 caagcatccg gggctggcag cctctggcag ggcaggcgca aaaccccctg gtcaggggtc
     4381 cgcgatgagc gactcggcgc agaagcccgt gggacgggtc atcggcaccg atccggccac
     4441 cccgctggag ttttgggtcg cggtggggga gggcgagttc ctccagctcg acgacgtgat
     4501 cgcctgcgag cgcgacgtgc cggggcccgg tccggtctcg atctatggaa tcgtttccca
     4561 ggttcgggcg cgccacgagg gcgccaagtt cgactcggat gtcttcttga tcgaggacgg
     4621 cttcctgccc gccgagatcg ccgaggccgc ccagatcacc accacccgct tcgagcccga
     4681 ggtcttcgtg ccgccgctgc cgggcgccga ggtccgccgc gcctccggca aggagcgcga
     4741 ccaggcgctg ttcttcgacg gcatggacaa acgcctcgcg gccggcatga gccgcgaagg
     4801 cgagccgatc ttcgccaacc tcgagttcct cgacggcacg cgcggggcgc acgtcaatat
     4861 cagcggcatc tcgggcgtgg cgaccaagac cacctacgcg accttcctgc tctacagcct
     4921 gttcaactcg aaggcgctcg gggcggaggc gatcaacacc aaggcgctcg tgttcaacgt
     4981 caagggcgag gacctgctgt tcctggatca ccccaacgcg atgctcgacg gcgagcaatc
     5041 ggaccgctac cgccggctcg acctgccggt cgggccgttc ccctcggtcg gggtgttcgc
     5101 gccgccgcgc cgcggcagcg ccgcagccgt gcccgacgtc gcctcgcgta tcgaaggcgt
     5161 cacgtccttc ttctggacgc tcgccgagtt ctgcgccgaa gagctcttgc cgttcctgtt
     5221 cgccgacgcc gaggacgacc ggcagcagta cacgctcgtc gtgcagaacg tcgcgcagcg
     5281 cctcaagcac gacgccgcgc cgctgcccga aggcggcatc gagatcgagg gcgtgttcat
     5341 ccgctcgttc cgcgagctgg tggacttcct gatcgaccgc gtgcaggacg aggaccacat
     5401 gccctactgg gcgggacgcg cgatcggcgc gggcacggtc ggcgcgttcg tgcggcggct
     5461 gcaggcatcg gtgcgccaca tcgagcatct ggttcgcggc gacgtcccgt acgccgataa
     5521 gcacaaggtc gcgctggaca agcaggtgac ggtggttgac ctgcacaacc tcaacgatcg
     5581 cgccaagcgc ttcgtcgtcg gcgtcgtggt gcgtcgcgcc ttcgaggcca aggagcggac
     5641 cggcgcggca aagccgctgc tgttcctggt gctcgacgag ctcaacaaat atgcgccgcg
     5701 cgagggctcc agcccgatca aagagatgct gctcgacgtc gccgagcgcg gccgctcgct
     5761 tgggatcatc ctgatcggcg cgcagcagac cgccagcgag gtcgagcgcc gcatcgtggc
     5821 gaactcggcg gtgcgggtcg tcggccggct cgacagcgcc gaggcgggtc gcgacgagta
     5881 cggcttcctg cccaaggcga tgcgccagcg cgcgaccatc ctcaagcccg gctcgatgat
     5941 catctcgcag ccgcagatcc cgatcccgct gctgctggag ttcccgttcc cggcgtgggc
     6001 gacccgctcg agcgaggccg gcgcgaaccc ggcgggcagc gatcatcccg acgatccgct
     6061 ggacgggctg ccgctgtgaa gatcctgcac acctcggact ggcacgtcgg caagactttg
     6121 cgcggccgca gccgcatcga cgagcacgaa gccgtgctcg ccgaggtcgc atcgatcgcg
     6181 gagcgagagc aggccgatct ggtgctggtg tgcggcgacc tgttcgactc ggccgcgccc
     6241 gcgcccgacg ccgaacgcgc ggtctaccgc gcgctgctcg cgctcaccga caccggcgcg
     6301 accgtcgcag tgctggccgg caaccacgac agcgatcgcc ggctgcacgc ggtcaagccg
     6361 ctgctcgagc tcggccggat cgtggtcggc atgccgtcgg agaacccggt cacgctggtg
     6421 cagggccgcg gcggcgacac cgcgagcctg gtgatgctgc cgtgggtcgg caagcagcac
     6481 gtggtcaagg tcgaagagct gatgaagatg gacggggccg agacccagca gcactacgcc
     6541 gagcgcgtca agcgcatcgt gcatcacctg tgcgcaccga tgagcgccga caccgtcaac
     6601 gtgctcgccg cgcacatgac tatcgccggc gccgagaccg gcgaagaggt ccgcagcgcg
     6661 cacgtgttcg actacgcggt tcccggcacc gtcttcccgg cctcgctgca ctacatcggc
     6721 ctcgggcact tccacacgcc gcagaaggtc gcagctccct cgctcgcctg gtattccggc
     6781 tccccgctgc ggctgggctt cggcagcgac gccgaggagc gtgccgacaa aggcgtggtc
     6841 atcgtcgagg cgacgccggg cgcgcccgcg gtcgcgcgtc tcgagccgct cacctcgggc
     6901 cggcggctgc gcacgattcg cggcacgctc gaccagctcg agaacatggc ccgaaacgac
     6961 atgaccggcg acgactacct gcgcgtgttc gtgcgcgagc aggccaaggc cggcatcgcg
     7021 cagcaggtcc gcgaatggtt cgagcacgca gtcgacgtcc agatcgaccc gcaggacgaa
     7081 ggcgagacgg ccgcgtccga atccacgcgc gcgggccgct cgcccggcga gctgttccgc
     7141 gactatctcg cccacaagca ggcgaacgac gagcgcgtga tcgcgctgtt cgaccagatc
     7201 ctcgaggaga tgcatgcggc cgacacggct tgagctcgaa ggattcaccg ccttcaaaca
     7261 gcccacggtc gtcgacttcg acggttgcga cttcttcgcg ctctgcggcc agaccggcgc
     7321 gggcaagtcc agcatcatcg acgcgatctc gttcgcgctc tacggttcga tcccgcggct
     7381 ggaccgcaac gacgtgcgca gcgtgatctc acaggggctg ctcgaatgca aggtgcggct
     7441 cgacttcacc atcggcgaga ccgcctacac ggcggttcgc gtcgtggcgc gcaccaagat
     7501 cggcgccacc acacgggagg cgcgcctgca gcgcggggcc gagacgctcg cgggcgacgc
     7561 gaaggcggtc agcgccgccg tcgaaggact catcgggcta actttcgagc agttcacgaa
     7621 atgcgtcgtg ctgccgcagg gcgagttcgc ccgcttcctg cacgacaagc cggccgaccg
     7681 gcaggatctc ctcgtcaagc tgctcggcta cgagctctac gagcgcatgg gccgccgcgc
     7741 caacgagctc gcgcgccggg ccggcgacca ggccgagtgg ctcgccaaac ggctcgagga
     7801 cgagttcatc ttcgctaccc cggagtcccg gaccgaagcc gccgagcggc tcgcgacgct
     7861 cgacgcggtg cgcgtgcgcg tcgaggatgc cacgccggga atcgaggcgc tcgacgccga
     7921 cgcgaaggaa gccaaggccg aagcggccgc ggcgcgggcc cggctggacc ggctcgccaa
     7981 agtctcggtt cccgacggcg ccggcgcgct cgccggtgcc gtcgcgcggg cccgcgacgc
     8041 gcgcgaggcc gccgaggccg aggccgaacg caccgacgcc gcccgccgcg cggccgaggc
     8101 cgcgctggag gcgctgccgc agcgctcggt gctcgagatc gcgcggcaag cgcacgcgga
     8161 ccgcgcgaaa tgcgagacgc agcgaacgca ggtgatggcg gccttggagg cgctcgggcg
     8221 ggtcgcgggg gaagcggccg tcgcgttcga tgcggccgtg gcggcgatgg aagcggccga
     8281 gcgagcgctc gaggcggcgc gcatccagca ccgcgcgcac gatctggcgc gttccctcgt
     8341 cgccggcgat ccctgcccgg tctgcaagca gaccgtgtcg acgctgcccg gcgacgacgt
     8401 tcccgccgac ctgcacgacg ccgagcgggc cctcgagcag gcacgggccg cgcgcgagga
     8461 cgccgaccgc aaccgccgca aggccgccga cgacaagacc cgcgccgagg tcaccgccga
     8521 cgagctcacc gccacgctcg acgagctcac ccgccgggtg gccgaccatc ccgacccgga
     8581 ccagctcgac cgggcgctca ccgagctgga gtcagccgag cgtgaagccg ccgacgcgcg
     8641 ccgcgccggc gacgccgccc gccgggccgc caccgacgcc cagcgccgcc tcgacggcgc
     8701 catgcgcgcc gagcaggacg cacgcaaagg attcgaccag gcccgcgacg cgctcgccga
     8761 cctgtcgccc ccgcccgcgt cccgcgacga tctcgccgcc gactgggagg agctggcggc
     8821 ctgggcggcg acgcagcatg ccgcacaggc cgctgccgcc gacgccgccg accgccgcgt
     8881 cgaggagatc ctcgagcagc gccgcgcgcg cgcgggcgag ctgctcgccg ccgcccggga
     8941 cgccgggatc gaggtgccgc acggcgtgaa gctctccgag gcgtgcatgc aggccgtcgt
     9001 gagccagcgc gccgtgctcg agcgcatcga agccgcgctg atccaaatgg aggacgtcgc
     9061 cgcgcagcgc aagaccgccg aggaagagca ggccgtcgcc tcgacgctgg gccgccacct
     9121 gagcgcgagc aacttcgaga agtggctgct cgacgaagcg atgcagcggc tggtcgaatc
     9181 ggcgagccgc gtgctgcgcg agctgtcggc gggcgggtat tcgctcgcgg tcgacgggaa
     9241 aggcaacttc gccgtgatcg accaccgcaa cgccgacgag cagcgaagcg ccaagacgct
     9301 gtcgggcggg gagacgttcc tggcgtcgct gtcgctggcg ctcgcgctca gcgaggacct
     9361 ggccggcatg gccgccgaag gcgcccggct cgacgcgatc ttcctcgacg aaggattcgg
     9421 tacgctcgac cccgagacgc tcggtgtggt gcagtccgcc atcgagagcc tcgcgagcca
     9481 aggccgcatg gtcgggctcg tcacgcacgt gcgcgagatc gccgactacg ttccggtccg
     9541 gttcgaggta cgcaagcatc ccgacacctc acgcgtcgag aaggtcgacg cgtgaggttc
     9601 accgtcgagc cttggtcgcc cgagtacgcg gcgcctttcg acagcgacgc cgcacccgag
     9661 ccgggcgcgc cgcccgccgc agaggtcgac gagcgcgtcg aagccgagcc cgcggagtgg
     9721 acgcccaagc cgccgccgca cggcgccgtc ccggcgaagc gggtcatgtt cgtcgacggc
     9781 gtgcagcgca tcgacgcctg ggtctgggtc gcggccgagg acgggacggt gcgtccgggc
     9841 atctgcgcct cctacgcggc cggcgtggtg tgctgcgacg gccgcgccga cgtgcccgac
     9901 atcgaggtgc gccgcggcct gttttcggcc gcgcccgtcg agccgatcaa cgcccgccgc
     9961 gtcgtctacc gcccctacgg cgcgacgggg gagagcgtcg atcagcttcg tcacgccgtc
    10021 cagcagcgca tgggcgagct cgaggccgac ctcgcccgcc gctgtggccc ggcggatctg
    10081 atcgtgctcg acggaccgtt gcgcggccgg cagaacgttc ccgacgcgat cggcttcgtc
    10141 aagagccacc agcagcgcta cctgtccgac cccgcgcacg ccgtggtcgg acggctcggg
    10201 gcaggggagc gcacgccgct gtttctgatg acgacctcat ggacgcgata ctcgtggtat
    10261 ctacgcctgc ccggcccggc cgcgcagccc tggtcctcgg tggtgcgctg cgaggcgtcg
    10321 gcggacctgt ccgcggccga ggcgacccgg gtcgccgatc gtgccgccgc gacgctgccg
    10381 atgttcgcct cggcggccca caaggatccg cgcgcgcccc agaatctgta tccgatcggc
    10441 ggtctcgagc gtgccttgcg acatcgcctg ggcgaccctg ctcttctgta ccgcgagctg
    10501 cgccaggcgg cgtcgcggcc tcgctctgcg gccgccgctc ccgctgggta gcgccggccc
    10561 cggccgcgtc cggggccgcg ccggcggacg ccggccggac gccggccggg agccggggga
    10621 gccgggaatc aatcgcctcc gatggagcgt tgcgtggggt gctttggtgg tgtataactc
    10681 gcggccctaa gggcaggatg gtggaaatag tggtcgacga cgaagaagaa acagaagctc
    10741 cggatgcgtc tttggacgag atcctcgcga agcgcgccga agaaaagggc gaagacgagg
    10801 aagaagaatc cgtcatcgag ctgacacgcg aagagcgtgc agagtcgctt gccatcaaag
    10861 tcctgccgca gcaggccgac gaattcacct gccgtaactg cttcctggtc aagcaccgca
    10921 gccagctcgc ggaccgcaag aagattatct gcaaggactg cgcctgaccc gtccggcctt
    10981 cgggcgaggc cgcccttgcg gtagtacctt ttaagcgtgt ccaccccccg gcctcctgcc
    11041 ttcctcgcct tttccggctg gccgctccgg ctgcgagttc cgctcgcgat ggcctccggt
    11101 gtcgtcgcca cgttcgcgtt tccgcctgct cagctgcggt tcgtcgcgtt cgccgcgctc
    11161 gtcccgtttc tcctgtcctt gcgccaggcg acggttcgtg acggcgccgt cgcgggcgcc
    11221 gcgttcgggc tggcgttcat gggcgtgctc atcggctgga tctcgatcgt cggatggatc
    11281 gcctgggccg ctctcgtctt cgcgcagatg ctgttctacg cgggcttcgg cgcactcgcc
    11341 gcactgctgg gccgtgcggg accggtcgcg cgggtgctcg gctggccgct ggtgttcacc
    11401 ggcttcgagc tcgcgcgctc gcgctttccg ctgggcgggt tcgagtgggg cgtgctcggc
    11461 tcctcgcagg aggcggtctc caccctcacg ccgctggcgc gcgccggcgg tgtgttcgcg
    11521 atctcgatcg cgctcgtcgc gatcaacgct ttgctgtccg aggcgatcgc cgcccgccgc
    11581 aagcgcaacg ccgtgctggc cgtcgcgctc gccgcggccg gcgcgatcgc tccggccatg
    11641 atgccgctcg gcgccgttgc gaactccggc accatcgacg tcgcgatggt ccagggcaac
    11701 gtgccgcgtg gatacttcgc cgggcttcgc cgcggacgcg tcggccccga ggacgagatc
    11761 atcatcgcca accatctgcg tttgaccgac acgctcgccg cgaacccgcc cgagctcgtg
    11821 atctggcccg agaacgcgct cgaccgcgat ccgttcaccg atcaggaggc gcggcgcgcg
    11881 atcgtggacg cgaccgccgc gacgggagcg ccgctgctcg tcggagcgat catcgacgga
    11941 ccccgtcccg gcacgttctt caacgccaac ctgctgttcg acgaaggcgg ccggctcctc
    12001 ggccgctacg acaagatgaa gctggttccg ttcggcgagt acgtgccgtg gcaatggacg
    12061 cgcagcgtcg tgccggcgct ctcggcgatc ggcagcgacg ggattcccgg cgacgagccg
    12121 gtggttctca acgccaacgg cacgcagatc ggcgcggtga tctgcttcga gtcttcgcgc
    12181 gccgagctgg tgcggcgctt cgttgagcgc ggcgcggaga tgatcgtggt ctcgaccaac
    12241 aacgcgacgt tcgagcgcac gccgctcgcg cgccagcatc tggcgctgtc gcgcatgcgc
    12301 gccgtcgaga ccggccgtcc ggtgctgcac gcggcgattt cgggcatctc ggcgatcatc
    12361 cagccggacg gctcgatcgt gcagcaggcg ggtctgttcc gtcccgcgat ccttcggcag
    12421 cgaatccaga cgacgagcgg cttgaccccc tacgtcgcct acggcgccac gatcgagctc
    12481 gcctacggca tcggcgccgc ggtcgccgcg atcgtggcgc tgctgttcat ccgccggcgt
    12541 ccctcgacgg tggccgagga cgacgagccc gacttctggc tcgcgccggt gacggcggcg
    12601 cacgacgagc cgcatccgct gctcgcccac gtcgacgacg cggaccattc gtccgcgccc
    12661 ccgcccgggc cttctcccgc gccctcttcc tctccggcct cggtggcgac caaggacccg
    12721 gccccgccgg cgcgggacga gccggaggcc gaggccgagc cgccgcgcgg tgacagggga
    12781 gcggaggccg cgcagtgaac gcgcttctgg tcctgccgac ctacaacgaa gccgccaaca
    12841 tccgagaggt cgtcgagcgc gcactcgcgg ccacgcccga tgtcgacatc ctcgtcgtcg
    12901 acgacaactc gcccgatggc accggccgta tcgccgacga gatcgccgcc gcgcagccgc
    12961 gcgtccgcgt gatgcaccgg ccgggcaagg gcggtctggg accggcctac atcgcgggat
    13021 tcaccgacgg gctcgcccgc ggctacgagg ccttcatcga gatggactcg gacctgtcgc
    13081 acgacccggc cgatctggcg cggttcgttg cggccgtcaa ggacgcggat ctggtgatcg
    13141 gctcgcgcta catccccggc gggggcgtca ccaactggag caagaatcgg gtccggctgt
    13201 cgcaggccgg gaacctgtac gcgcggacgc tgctcggctt cggattgacc gactccacga
    13261 gcggctaccg ctgctaccgc cgcggcttga tcgagacgct gccgctgcgg cagatcggca
    13321 gcgagggcta cacgttccag atcgagatgg cgtggcgctc ctggacgctc ggcttcatcg
    13381 tcaccgagat ccccatcgtg ttccgcgagc gcaccgaagg ctcgtcgaag atgtcgcggc
    13441 ggatcgtgtt cgaggcgctg tggcgcgtgc tggtcttcgc gctgcgcttc aagcgtgcgc
    13501 cgcggcgcgc gcatcccaag agcgtcgcga catcgccggg ataggaaggc ccggcaacgg
    13561 cagcggccgc acccccgatt cgagtgcggc cgcagccgga gaggcatccg cccttaggtc
    13621 gtgcgaatcc acagaacctg accggtcgcg ccgcgcacgc ccacgattag cgtgcgctca
    13681 cgcgtgccgc tgcgctgcag cacggtgatg acgatgtcgc cggttccgtt cgggttcatc
    13741 gactgcgtgt gcacgtcgcg cacgcgctcg gtaccgccgc cgaccagaac ccgggaggtc
    13801 cacgccgggg cgccgttcgc gccggaggcc gtcttgatca ccaggctgtc cgaggtgcgc
    13861 gtcacggact cttgcacgtc cacaatgtcc gccgcggcgt cgccgacgac gttgccgccg
    13921 acgggcgcgc ccgggtcgag cgtcgaggtg cggctccaca gcagcgcgcc ggtctgcgcc
    13981 gacaccgccg cctggctcca ctgcgtcgcg agcccgtcgg acgggaccgc attgaccgcg
    14041 tggaccatgt cggggtggac gtcggcgtcg atccgaccgg cggacgggct cagcttcgcc
    14101 gaccagtcgc cgatgccgcc gccgttcgcg acgggaacat ctcgcgagta caggctggtg
    14161 ccgttcgctc cgccgagcgc ccgatagacg accttcgacg ggatccggcc cggcgcggac
    14221 acgtgcaggc cttgcgtctg cacgtccagg ccggccgcgg cgtcgaacga gccgccgacg
    14281 aacgcgatcg cggcttcgtc gagcgtccgg gaccacaagc tctgccccag cggcgcggtg
    14341 cgcaccgcga cctgcgtcgg gccggtgccc ggcaccgacg ggtagcggtt gatcaggatg
    14401 tcggcgaggc cgtcaccgtt gaggtcgtgc cgcgcgtcga acacgcccac gtagcccggc
    14461 gccaccgagt cggacgcggc gaacaggggg acgccgctcg cggtaaacgc gcggtaggtg
    14521 accaggttgc cgttgccggt cgcaatctcg tcgacggcga gcagcccgcg ggatccggtg
    14581 ccgaggaagt cgccgccgac ggcgacggcc gggacggcgt cacggccgac cgcggtgacc
    14641 gaggcgagcg gcgcgccgct cgcggccgcc accgtcgtca gggtcgatac gacggcggtc
    14701 gttccggcgt gatggaacgc gaccgtctgg gtcagtgcga agtcgatgct gccgtcaccg
    14761 tcgacgtcgg ccggcgccga gccggtcacg taggcggcga acggaaccgg gctgtcctgc
    14821 gacgccgccc gctgacgggc cgattcggtc ggcggcgaga tcgtcggcgt cggcaggccg
    14881 ctgggaaccg ttgtcggcag atcggacggg atctcggagg ggatcccgct cggaagctcg
    14941 ctgggaacct cgctcggaag ctcgctggga acctcgctcg gcaggccggg cagcggcggc
    15001 aggccggacg gcggcgtcgt ggggacgtcg ctcggcagga ccgacggcag gcccgatggc
    15061 ggaatgctcg gcacggccgg cacgccggac gggatgacgc tcgggatcgc gctcgggctg
    15121 accgagggga tcgacggcgg gctcgacggc ggcacggtgg gaggcgtggt gtcgggcgtg
    15181 tcggtcggca gcgagccgcc ggtcgcccag atgtcgttgc cggtcgcgcc gtccaccgcg
    15241 tgcagcgtcc agcgcagctc gcggtcggct tcgtgctgct cgagcaggta gacgtccatg
    15301 gcgccgccgt tgatctgcgc cgggtacacg gcgacgtgga tcgtggacgg acgcacgatg
    15361 ggggccgcct gccagatcgt cgacgcgttg tggctcgaca gcaggcgata gctcagcgtt
    15421 ccgttggtcg cgcggaagtc gatcaagtcg gggcggttgt cgccgttggc gtcgccgatg
    15481 ggaacgacca ggcgcccccg cgtcccgctg ccggacagcg acgccgcgct ctgggcggcg
    15541 ttcacctgcg cggccgagac cttctggccg ccggcgagcg gcgcaagcgc cgcgggcatc
    15601 gcggccgggg ttgcggatag gtcgacgcgt cccgaggtgg cggtgaggat ctggtcgagg
    15661 gtcgaggacg tcggggcgcc ggtcgcgagc gcggccgcga gggccagcgg ggcgatcagc
    15721 caccgggaaa tcgtgcgggt cgtcagcaac actatgggcc tccggtcggc gcaggcttct
    15781 gctctcggcc tgacaacgac gcggccacgc aagacgtgac gcactgtgcc gcaggatccc
    15841 gattcgtccc tgaaggattt ccgggccgcg agccgaccgg ccagatcggt ttccgcgcga
    15901 aggcaaggaa accggacggc ccgggccgaa tatgatcttc aacgctatgg gaggctttcg
    15961 ggcatcacgg ctcgggctgg tgctgcttgc cgcgggaacc ggaaccgcgg cgcacgtcgc
    16021 cggctacttc gccgtcttcc acgacgccca cggccgcgcg gaggcgctcc atcgctcggg
    16081 ccacgattac ctcgcgttcc tggcagttcc gctcgttctc gccgccgcgg gcgggcttgc
    16141 cttgtcgctt cgtcgaggtc tccaccgcac cgcacgaccc gtcgcccaag ccgaggcctt
    16201 ccggttcttg ctcggcgtcc aggtggcggg ctatctcgga agtgagatcc tcgaacgtgg
    16261 gctcacgggt gagcttcttg ctcctgagac gctggtcgta tgggcggccg gcgccgcctt
    16321 gcaagtcgtc ttcgccgcgg cgggcgccct gctctacgtg gcggccgagc gggtcggagt
    16381 cgccgtcgcg tcggcgcgcc gcgtccgtgg tcgagactcc cacgtccgtt cgcggccgcg
    16441 gagcagccgg ccgcgacgca tcgcgcctct gatccggctc gcgccacgcg ccccgcccgc
    16501 ctgattcctc tcgcgacgcc tccgaacgcc cggacctgag cctctcacgt ccgcgttcgg
    16561 tcgcactcgc catcgatcga tcgtgcgcgc gccccgcgcg ccgcacgatg tggatgcgtc
    16621 tcctcccgga tcggtcgccg ctcgtggcgg cctcgggcgg cgtgcatcca cccgatgccg
    16681 cggcgtcatc gccgtgtcaa gcccctacgc acgggacggt cccgtgcgtg cgtccggaaa
    16741 ggaaatgcag atgaagaagc tgtcggccgt catgattgcc ctcgtggtgt cgctcgccgg
    16801 atccgccgcc ctcgccgggc acggcggcaa aacctactgc gaggaaaaca acaagcgcaa
    16861 cgggatgctg accaaggatg tgcacgagat gcacgaccgc gtgaagccga tcagtcccga
    16921 actcgccgaa atcatccacg acgaggtcga gcctcgtacg tgcgagtaac agccgctcgc
    16981 cgcggacgcg ccgtccccac aaggacggcg cgtctcgcgg ctgtgctggt cgtcgcttcg
    17041 attgcggcga ccccgggctt cgcggactcc ggatgcgcgg ccgtgcaccg ctatccggtg
    17101 tccggagccg agtggatcgt tcgcgacgtt cccgagtttc cgcacggcga tcaggcgctt
    17161 cgcgcgcacg ccgtcgatcc tgaacggcct gaccggatct ggggaaccaa cgggcattcg
    17221 gtgatgcgca ccgtcgacgg cggctgcgag tggcgcgagg tgctgcgcgc cgggcccggc
    17281 tccgagatcg tgcgtctcgc agcgtccgcg gacggccaca cgctgctcgc gatgcaggag
    17341 gtaggtgcgt ccgaggtggc gatcgcagcg gcgtcgggcg tctcgatcgc cgtgtcgcgt
    17401 gacggcggcg aacgatggag cgatttcacc gctctggccg gcaacggccg cggtggtccc
    17461 gtcgcgatcg cgtcgtctca tcccggaatc gcctacgcgg ccgtgggcag cgtcctgtgg
    17521 cgttgggggg aaggggactg gcagccggcc ggacccgtcg cggagccgcc caagacgccg
    17581 gatctcgagc cggtcatccg ccggattgag atcgatccgt tcgacccgaa tcgtctctac
    17641 gtcaaaggct cgtactcgct gttcgtctcg catgacgcgg gggagtcctg gcggcggttc
    17701 gaccggagcg gcgatcctgc cggctccggt ctgttcgggc cgttgctcgc gcgcccgtcc
    17761 gcatcgatgc cggcctccat gctgctgatg cgcggcgcgt accccacgtc gccgatccag
    17821 gagtacttcg agtcgcgaga cggaggtgaa acattcgccc gatacgacac gagccgcttc
    17881 ggcggattca tcggcggcgt tccggtcgca ggcgccgcgc gcgctccgga cgacgtgctc
    17941 gtggcgaccg atcagccggg cggtggcgcg gcgcacggcg tctggcgcct ggatccggcc
    18001 acgcgacgct tcgtcgacgt cgatcgctac ggcttcgggc cgttcaacca ggtccacgcg
    18061 ctcgccggat ccggaagcgg ctattcgctt tcctcgccat cgacgattgc cacgtatcgc
    18121 gaccgcggac gtcctccgtc attcgtcgcc cgcgagcttc cgccgcttgt gcacatcccg
    18181 ccgttcgatc cgccgccgcc tcccccggtc ccgccggcga cgctcgacgg cattccttcg
    18241 cgcgttgcgc tcgaggccgc cgcgacggcg tccttcggtg tcggcgtgtt cgtgccgccc
    18301 gcgccgacgc cgatcgacgt gttctttctg gtcgatacct cggggagtca gacgccgtcg
    18361 gttcgcgggc tcgcgaccgg gatcgacgat atcgtgcggg cgctcgccga agccggcatc
    18421 gacgcgcagt tcggtctcgg tgagttccgc gacgtcaaga cgctccggta ccggcgccgg
    18481 gctgatatcg gcccgcccga tcgccggttc caataccagc tcgaacgcct ccataccgcc
    18541 ggcggcgttg aagagccgca cctgaccgcg ttgcatcagg ttgcgaccgg cagcgggctg
    18601 cctgccgtcg gcgactcgct ggccgtgccc gccggccagg acgcgacttg gcgccgcggc
    18661 tcgcttcgta ccgtgatcct gatcagcgac gaacccttcg ggaccgatcc caatggtccg
    18721 tcgcgcgata agaccatcgc ggcgctgcgt gccgacggag cccgggtgat cggcgtcgag
    18781 atcgacccgc cagccgaagc ggctgtgttc gacgccgccg gacgtacctt tgcatccacg
    18841 ctccgacggc agatggaaga tgtcgcacgc cacaccggcg cgttcgctcc tgccggcggc
    18901 gtggactgca cgggcaacgg aaccttcgag gtcgaagagg gcgcgccgat ggtatgcagc
    18961 attccggtcg acgaggccgc aggtgtcgcc cggctggccg acccgctcgt ccgcatcctg
    19021 tcgcagctcg ccgacatgca gacggtccgg cttgccgcga tgccgccgga cggcgtgacc
    19081 gcaaccgtcg atcccgcgac cgggattgcc ggcgtggacg tcaagtctgc gtcgcggcac
    19141 gcgttcacgg tcacggtcgg ctgccacccg gggcgcagcg tcgccgcgcc actcaggatt
    19201 gaggccgtcg tcgccgagcg ggtgcgggcc acgcagcaca ccctggtcga gtgcggcgcc
    19261 gcccctccag cgcccgtcct gccggggccc ggcccggcgg ccgccctggt gccacccgcc
    19321 gcggcccagg ttcaacccgc atcaccgaac acgccgaact acatggccaa tcccggcgcc
    19381 tacggtcaga tccaggtcaa tccggcccca cgcgacgagc acgaggtcca ggaaaaccgc
    19441 cagcacgagt tcacggccgt ccgtcgctcc cgagatccgg cttccggcct ctacgcgg
//
LOCUS       DATLFO010000201         4940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_57909, whole genome shotgun sequence.
ACCESSION   DATLFO010000201 DATLFO010000000
VERSION     DATLFO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57909"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..800
                     /locus_tag="VM841_08335"
                     /pseudo
     CDS             1..800
                     /locus_tag="VM841_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1108..1914)
                     /locus_tag="VM841_08340"
     CDS             complement(1108..1914)
                     /locus_tag="VM841_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HVL90226.1"
                     /translation="MHRRLLALGLVFSVTQVSAQTPAFEAASVKPNASGAAAQSGRMA
                     NGSVTMTNMRVRALIVTAYGSRPERIAGAPSWIDEARYDITAKAPAGTPDNQLPLMLR
                     TLLTDRFKLVARTEMREQPVYALVMVRPGGTPGPSLRPSTECESGGAGSGIGGPPLPA
                     GKRACGVISGSDGKAAYIIGGARSMELLARALQNLGLIERPVIDRTGLTGTYDFDLRF
                     SAAPLASSAADVPLPSIFTAVQEQLGLRLEPARGPVEFLVIDSIERPTAD"
     gene            complement(2464..3066)
                     /locus_tag="VM841_08345"
     CDS             complement(2464..3066)
                     /locus_tag="VM841_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVL90227.1"
                     /translation="MSRTASFVLALLLAAAPALAQTWQIDPAHSRAMFTVRHLTIADI
                     RGDFGAVTGTVEYDGKDLTKAKVTATIDVNSITTRVAARDEHLKTDDFFDVKNHPTMK
                     FVSTSITPKGNGRYDLTGDLTIRGVTRRVALEMTAPAGPVTVRGDTRLGASASGTINR
                     KDFGIKYHELLDNGGLAVADNVLIQLDVEVIQQKAATSSN"
     gene            3729..4106
                     /locus_tag="VM841_08350"
     CDS             3729..4106
                     /locus_tag="VM841_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90228.1"
                     /translation="MMRVQLTGLVLLALASAPAAQEQGSAPPAAPAQPEAPAQPAEKS
                     PFDLFKRQQPLIQPNDLRVFPPNAVRAYQTDERPSPPPAHCFTMRIVPADPSIDPKFV
                     RPIPETDIKYSGRVIPLPGPACR"
     gene            4455..>4940
                     /locus_tag="VM841_08355"
     CDS             4455..>4940
                     /locus_tag="VM841_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90229.1"
                     /translation="MRRLLTIAALVLVIAVVHAVRTHAQGNTGCDRACLEGFVDRYLD
                     ALVARDPARLPLAANVRFTENGVRLNVGDAQWKTVIGKGSYRLFVTDPEAGQVTFIGT
                     VVEEARTPGGSPTAIALRLKVENRQIAEIETLMIRSTAAQNTGRGAGGAPASAFTGAA
                     VN"
BASE COUNT          850 a         1619 c         1611 g          860 t
ORIGIN      
        1 cctactttct gcggaccgcg cagcagcatg cgccggagac acgctcggcg cgggcgcggc
       61 gggatgacga actgcgcgtc gccattcggc gcgtgtggga tgaacaccag caggtgtatg
      121 ggccgcggaa agtgtggcgc cagctgcgac gcgagggcgc gcgggtggcg cggtgcacgg
      181 tcgagcgtct gatgcgcgag atgggcttgc gcggtgcggt gcgcggccgg gcctggaaga
      241 tcaccaccca cgtggatccc gccgcggccc ggcccaccga tctcgtcgat cgtcagttca
      301 ctgcgactcg cccgaatcag ctctgggtgg ctgacttcac ctatgtcgcg acctggcgcg
      361 gctttgtcta tgtcgcgttt gtcgtcgatg ttttcgcccg ccggatcgtc ggctggcgcg
      421 tgtcggcctc gctgcgcacc gatttcgtcc tcgatgcgct cgagcaggcg atttacgacc
      481 gctgcggccc cggcgtcgat gatctcgtcc atcacagcga tcgcggcaca cagtacctat
      541 cgatgcgcta tacggaacgg ctcgccgagg ccgacatcgc gccgtcggtc ggcagccgcg
      601 gcgactcgta cgacaacgcg ctcgccgaat cgatcatcgg gctgttcaag acggaagtca
      661 ttcagcggaa gggaccatgg cggcacctcg aggccgtcga attcgcgacg ctcacctggg
      721 tcgactggtt caacaaccgc cgtttgctcg aaccgattgg ctacgtgcca cccgcggagt
      781 acgaagcgca gtactacagg gatgctgatg cacagggtgg cgctcgccgg catccagata
      841 ccctcaacga gattgacgcg atcgccgccg agcgcccgtt ggcctttcta cgaccactgc
      901 agaatgaggt actgtcacta ggcccgacga cgctgaaccc taactcacac taaccgctct
      961 ccgataaacc cggtgcactt catttgcaaa ccgtacgaag tgatttgcaa acgccgaaga
     1021 acatttgcaa caccgcgaca gtgatgtgca acacgccgca gacaatttgc aaaccgggat
     1081 caccgtcttg caaccgcctg gcggcgccta atctgccgtc gggcgctcga tggagtcgat
     1141 gacgaggaac tccacgggac cgcgcgcggg ttccagccgc agcccgagct gctcctgcac
     1201 ggcggtgaag atcgatggca gcggaacatc ggccgctgag gaggcgagcg gggcggcaga
     1261 aaaccgaaga tcgaagtcat aagtgcccgt caggccggtc cgatcgatga cgggccgctc
     1321 gatcaggccc aggttctgca gcgcgcgggc cagcagctcc atcgaacgcg cacctccgat
     1381 gatgtaggcc gccttgccgt cgctgcccga gatgacgccg cacgcgcgct tcccggccgg
     1441 caacggcggg ccaccgatgc cagacccggc gcccccggac tcgcattcgg tggacggtct
     1501 gaggctcggt cccggggttc cgcccgggcg aaccatgacg agggcataca ccggctgctc
     1561 acgcatttcc gttcgcgcca cgagcttgaa ccgatcggtc agcagcgtcc gaagcatcag
     1621 cgggagctgg ttgtcgggtg tccccgccgg cgcctttgcc gtgatgtcgt accgcgcctc
     1681 gtcgatccac gacggcgcgc cggcgatgcg ctcgggcctg ctgccgtagg cggtgacgat
     1741 gagggcccgg actcgcatgt tggtcatcgt gacggagccg ttcgccatcc ggcccgactg
     1801 cgcggccgcg ccactcgcgt tcggctttac cgacgcggcc tcgaacgcgg gtgtctgggc
     1861 agagacctgc gtgacactga acaccaggcc aagggcgagc aacctgcggt gcatgaggcg
     1921 tcctcgtgac tcgtttgacg gcgcgcgtga gcggcgggtt ggctgggggc cggcgccgct
     1981 ctcgccttca cgacgttcgc aaatcatgca gcgccaggaa gtttcacggc ccgagcgctt
     2041 cctgggtgcc agtgtgaaac cggcggacca ccgcgcttac acccgccgtc cgtagaggcg
     2101 aatctccaga cccgcgtcct tcaagactgc ctgacccgaa gccgcccact cgggcggtca
     2161 gctccgcgcc ggggtgcttg gatgtcggct cattcgacac gcaccatctt caggatgatc
     2221 tttcgaccgc gagacgtcgg ggccacttga ccggcaccga gagccgtcga gagcccggcc
     2281 accgcgacta tcactccaca cccgagaccg gctacaaacg accgcatgtc cagcatcatt
     2341 ttgcccctcg acaaacgcat acacaggaat cggcggccgt gattcccaag cacaacagga
     2401 ggctggggct cggctatcgg catcacggct gaaaccgggc gcccgtcggt tggagccgcc
     2461 cgttcagttc gacgacgtgg cggccttctg ctgaatgacc tcgacgtcga gctggatgag
     2521 cacgttgtcg gccaccgcga ggccgccgtt gtcgagcagc tcgtggtact tgatgccgaa
     2581 gtccttgcga ttgatcgtcc cgctggccga cgcgccgagc cgggtatcgc cgcggacggt
     2641 caccggcccg gcgggggcgg tcatctccag cgcgacgcgc ctggtgacgc cacgaatcgt
     2701 caggtcgccg gtcaggtcgt agcggccgtt ccccttgggg gtgatcgacg tcgacacgaa
     2761 cttcatcgtc gggtggttct tcacgtcgaa gaagtcgtcg gtcttgaggt gctcgtcgcg
     2821 tgcagccacg cgtgtggtga tcgagttgac gtcgatcgtg gcggtgacct tcgccttcgt
     2881 caggtccttg ccatcgtatt cgacggtccc cgtgaccgcg ccgaagtcgc cgcggatgtc
     2941 cgcgatcgtg aggtgccgga cggtgaacat cgcgcgcgag tgcgcaggat cgatctgcca
     3001 ggtctgcgca agggccggtg ccgccgccag gaggagcgcg agaacgaaag aagcggttcg
     3061 tgacatgcga atgattgtag ggcatgaatg tcgccgctcc gcctggccca gacaaatcga
     3121 caaggggccg cagccgccac cagtacgcag cggatctgca agagacgatc ttccacaggg
     3181 ccaaccaatt cgatacaaag gagatattgg ggggaggggc cttcaccgaa tcttcgaggt
     3241 gctggaagga gaactcacgc ttcgagtcgg aagacgtacg acacggctga cagtcgggga
     3301 acaacccgac gtgtgagcgt tcgacgccga agagccgctg ggcgcacagc tacccaatcg
     3361 tagaggcgga ccttcaggtc cgcctccttc gagactgcct gacccgacgc gatccgcttg
     3421 catcgtgagt acactgcgac cgatgtcgag gtcggagaga tccgatcacg tcttccccgt
     3481 cgtcccggtg cggcagtggg tgttgagcct gccataccgg ctgcgatacg tgctcgcctg
     3541 ggaccacgcg ctctgccgtg cggtcacggt gttttcgtgc gcgccgtgct cggatcttgg
     3601 ccagatcgga tgtcgaacag cgtggccatg acgggataca gtgatcgtaa tccgggggtc
     3661 accccggccg tgatccccac ggcgagtaag ccgtaagcag cctgcggccg tctaagatga
     3721 cggaggtcat gatgcgcgtg cagctcaccg ggctcgtcct gctcgctctc gcgagcgcac
     3781 cggcggcaca ggagcagggt tctgcaccac cggcggcgcc ggctcagccc gaggctcctg
     3841 cgcaacccgc tgagaagtcg ccgttcgatt tgttcaagcg ccaacagccc ctgatccagc
     3901 cgaatgacct tcgggttttc ccgccgaacg cggttcgggc ctatcaaacc gacgagcgtc
     3961 catccccgcc tccggcccac tgcttcacga tgagaatcgt tccggccgat ccgagcatcg
     4021 atccgaagtt cgttcgtccg attcctgaaa ccgacatcaa gtactccggt cgtgtgatac
     4081 cgctgccggg ccccgcctgc cgttagctgc tgccgtgctc ggggccatcg gcgctgtgca
     4141 gggtagggct ggcggcgggt cgcgtaaggg gccttcggta aggttccccg gctaatcgct
     4201 cctctagaca aggcgggcac ttcggccagc caccggccgt cgtcttcccg ctctatttca
     4261 atcgtgaacg tcacgacggc aatgtgccat caccgccgga cctcaaccac acatcgggtg
     4321 cggtgttcga cctggctcaa tgccggcggc ccggaggaga gcaccggtct ttgctcaggc
     4381 agtgccctct tctgtctgaa ggtggcgacg tccattgctt gacatcgccg cgaggggctg
     4441 caagcattcc cgccatgcga cgtctcctga ccatcgctgc actcgttctt gttattgcgg
     4501 tcgtccatgc cgttcgcacg catgcgcagg gcaatacggg gtgcgatcgt gcgtgcctgg
     4561 agggcttcgt cgatcggtac ctggacgcgc tcgttgcgcg cgacccggcg aggctgccgc
     4621 ttgccgccaa cgtgcggttc acggaaaacg gcgttcggct caacgtcggc gacgcgcaat
     4681 ggaaaacggt gatcgggaag ggcagctacc ggctgttcgt gaccgatcct gaagcggggc
     4741 aggtgacctt catcggcacg gtggtcgaag aggcgcgtac accgggcggg tcgccgacgg
     4801 ccattgcgct gcgcctgaag gtcgagaacc ggcagattgc cgagatcgaa acgctcatga
     4861 tccgcagcac cgccgcgcag aataccggac gcggcgcggg cggcgctccc gcttccgcgt
     4921 tcaccggagc cgcggtcaac
//
LOCUS       DATLFO010000202         5343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_106788, whole genome shotgun sequence.
ACCESSION   DATLFO010000202 DATLFO010000000
VERSION     DATLFO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106788"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1035
                     /locus_tag="VM841_08360"
     CDS             193..1035
                     /locus_tag="VM841_08360"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HVL90230.1"
                     /translation="MSIELPDILTRGSASDRGRRAVEILARWGLAAIGVSYLLVAGIS
                     LKLAISGSGQASDREGALARIAQTGWGVPALIVIAIGFAGYAAWRLVLAITGENVEND
                     DDKHPLKRVGYAARGLLYAVLTFVTLEMAFGGSGGSTGGGSGEEKQQASTVLGWPGGR
                     WIIAGIGLAFVAGAIFNAYRAVSRSYKDDLKTFEMPDDKERVVTAVTAFGLFARMTVF
                     GIIGWFLLRAAVEHNPDKAVGLDGALQALAGEAYGRVLLAVVALGLVAYAAFRFIEAR
                     YRTV"
     gene            1134..1232
                     /locus_tag="VM841_08365"
     CDS             1134..1232
                     /locus_tag="VM841_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HVL90231.1"
                     /translation="MGSIIKKRRKKMRKHKHKKLLKKTRWQRRQKS"
     gene            1334..2170
                     /locus_tag="VM841_08370"
     CDS             1334..2170
                     /locus_tag="VM841_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HVL90232.1"
                     /translation="MALAFRFITFLTDYGLEDEFVGVCHGVIKRFAPDVEIIDISHGI
                     PPQDVTAGANVLGQAVPYMPPAVHLAVVDPGVGTHRRCVVIGTKDGPPLVGPDNGLLW
                     IAAQRLGGATQAHEITNAELFLRTPSNTFHGRDVFSPVAARLALGMPPEEVGPRLDLE
                     QVVKLEIPQSKVDDDHVHGLVVQADHFGNLQLNVHRQDLEGAGIMLGDDLELRVGGRA
                     YTIPYRQAFAEVPQGKLALLEDSYRHITIIVNKGHAARFLEARRGDPVILARHPKAAS
                     RP"
     gene            2244..3293
                     /locus_tag="VM841_08375"
     CDS             2244..3293
                     /locus_tag="VM841_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL90233.1"
                     /translation="MKQRVLVTGVSRFLGGRLAQRLEQDPNIEEIIGVDIDEPHVDLT
                     RTEYVRADIRNPLIVKVLQATGVDTVVHTSVIATPGRVGGRTNMKEINVIGTMQLFAA
                     CQKAEKLRKVVMKSSTAIYGSEPSDPGVFSEEMSPRSHPRTGYAKDSVEVENYARSFG
                     RRRTDVSLTILRFANFIGPQIDTPLTRYFGLPLVPTALGFDPRLQFLHEDDAIEILYR
                     ATVEDHPGTFNAAGDGVLYLSQAIRLGGKVPVPVPLPFINMVAAAVKRSGIVDFSQEQ
                     VRLLLYGRVGDVSRLKNVFGYEPQYSTREAFADFVARRKLAKLFTPEQAAQWERELYR
                     FLARRKAGAAWQTRA"
     gene            3275..4282
                     /locus_tag="VM841_08380"
     CDS             3275..4282
                     /locus_tag="VM841_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL90234.1"
                     /translation="MADARVIPIHLAESPRCAAQTADGRPCRNRAGESGYCARHEPEA
                     AARDRHPTGASAEPNALQQWLEEVLAFVRRRMTGDYPVDDMGFDRDLTEHALLPLLRP
                     LYRSWWRIDQIGVENVPAEGPVLLVSNHSGTLPFDALMMKLGIFEETGRHVRLLAADL
                     ALQLPVVGELARKSGNTLACEDDALRLLRAGEVLGVFPEGFKGIGKPFRERYRLQRFG
                     RGGFVEVALRAGVPIVPVAIVGAEEIYPIIANVKPLARLFNFPYFPVTPTFPLLGPLG
                     TLPLPSKWIIEYGEPIHTEGFGTEAANDPMFVFNLTDQVREVIQQTLFKNLIQRKNAF
                     F"
     gene            complement(4329..4964)
                     /locus_tag="VM841_08385"
     CDS             complement(4329..4964)
                     /locus_tag="VM841_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90235.1"
                     /translation="MNRASRKLAVAGVMTALAAVAFAPAARAGTPISLTSVSPTAGVW
                     GTTFTMNGTTAANRQVRVQRMYVGANQWQDVTTTTSDDDGKFSTADQPLAHAWYRAFL
                     TSDLSTSNWVKAQVRPGVHLGVGRSGEQAVFSGRVLPGHPGSRVLLQMFDGINKEWVT
                     VKTARLNSSSFYRINYKATDNGRRLFRVAWPTQDNNHLWNLSAWVWVTWTR"
     gene            complement(5118..>5343)
                     /locus_tag="VM841_08390"
     CDS             complement(5118..>5343)
                     /locus_tag="VM841_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90236.1"
                     /translation="SLHLGLAPKLLSGAPLFAVSIDGPASVADVRGGNVLGVKEVRIP
                     TTGVSDTAMLLVGTLMVGLAIGARKFALSK"
BASE COUNT          848 a         1810 c         1813 g          872 t
ORIGIN      
        1 ctgttcgtct cggactgagc gtcttgcgag gcccggcgaa cgccggacgg cccggcgctg
       61 ccccggcccc ggcccgtgcg gtccgagcgt gtcgccgcgt cgcgcacgac atcgggaggc
      121 tccgcgcaaa gtggcgtgtg ccgcccctcc ccgatggttc gcattcccgc acagcagggt
      181 aatcacgatg cgatgagcat cgagctgccc gacatcctga ctcgcgggag cgcctccgac
      241 cgggggcgtc gcgccgtcga gatcctggct cgctggggtc ttgccgcgat cggggtctcg
      301 tatctgctcg tggccggcat ctcgctcaag ctcgcgatct cgggcagcgg ccaggcgtcc
      361 gaccgcgaag gggcgctggc gcgcatcgcg cagaccggat ggggcgtgcc cgcgctgatc
      421 gtgatcgcaa tcgggttcgc cggctatgcc gcgtggcggc tcgtgctggc gatcaccggc
      481 gagaacgtcg agaacgacga cgacaagcat cccctgaagc gggtcgggta cgcggcccga
      541 ggattgttgt acgcggtctt gacgttcgtc accctcgaga tggccttcgg tggatcggga
      601 ggctccaccg gagggggatc aggcgaggag aagcaacagg catcgaccgt gctcgggtgg
      661 ccgggcggcc ggtggatcat tgccgggatc gggctcgcgt tcgtcgcggg ggcgatcttc
      721 aacgcctacc gggccgtatc gcgatcgtac aaggacgacc tcaagacgtt cgagatgccc
      781 gacgacaagg agcgggtagt cacggcagtc accgcattcg gcttgttcgc ccgcatgacc
      841 gtgttcggca tcatcggatg gttcctgctg cgggccgccg tcgagcacaa tcccgacaag
      901 gccgtcggcc tcgacggagc ccttcaagcg ctggccggag aggcctacgg ccgcgttctg
      961 ctggcggtcg tcgccttggg cctcgtcgcg tacgcggcct tccggttcat tgaggcgcga
     1021 tatcgaacgg tctgagccga ggattgagcg tcccgcaacg ggcctgtaca attccgtgcg
     1081 ctcgtgaaac ggacggccgg gagtccctgg ccccggctac cgaagggaac agcatgggtt
     1141 cgatcatcaa gaagcgccgc aagaagatgc gcaagcacaa gcacaagaag cttctgaaga
     1201 aaacgcgctg gcagcgccgt cagaagagct gaccggccgc gccgaaacga aaaggggact
     1261 ctgcggagtc cccttttcgt ttgcctttgc cgcctcttca cccgcggcgg aggcggcggt
     1321 atccttgcgg accgtggcgc tcgcgtttcg gttcatcacg ttcctgaccg actacgggct
     1381 cgaggacgag ttcgtcggtg tctgtcacgg tgtcatcaaa cggttcgcgc ccgacgtcga
     1441 gatcatcgac atctcgcacg gcatcccgcc gcaggacgtc accgccggcg cgaacgtgct
     1501 cggccaggcg gtgccgtaca tgccgccggc ggtccatctg gccgtcgtgg atccgggcgt
     1561 gggaacgcac cgccgctgcg tggtgatcgg gaccaaggat ggtccgcccc tggtcggtcc
     1621 cgacaacggg ctgctttgga tcgcggcgca gcggctcggc ggcgccacgc aggcccacga
     1681 gatcaccaac gccgagctgt tcctgcgcac gccgtcgaac acctttcacg gccgggacgt
     1741 cttctcgccg gtcgcggcgc gtctagcgct tgggatgccg ccggaggagg tcgggccgag
     1801 gctcgacctc gagcaggtcg ttaagctcga gattccccag agcaaggtcg acgacgacca
     1861 tgtgcacggg ctggtggtgc aggccgacca cttcggaaac ttgcagctga acgttcaccg
     1921 gcaagacctc gaaggcgccg gcatcatgct cggcgacgac ttggagctcc gcgtcggcgg
     1981 ccgcgcctac accatcccgt accgccaggc gttcgccgag gtgccgcaag gaaagctggc
     2041 gctgctcgag gactcctacc gtcacatcac gatcatcgtg aacaaggggc atgccgcacg
     2101 attcctcgag gcgcgtcgcg gcgacccggt gatcctggcc cgccacccga aggccgcttc
     2161 gcggccgtag gcgcgctcgc cggcgcctcg ggccgcaggt gcgctcgctg gcgcctcgca
     2221 gcccgcgcgg ttagtctcct gccgtgaagc agcgcgttct cgtcaccggc gtctcccgct
     2281 tcttgggggg ccggctcgcg cagcgcctcg agcaagaccc gaacatcgaa gagatcatcg
     2341 gggtcgacat cgacgagccg cacgtggacc tgacccggac cgagtacgtg cgcgccgata
     2401 tccgcaatcc cctgatcgtg aaggtgctgc aggccaccgg cgtggacacc gtggtgcaca
     2461 cgagcgtgat cgccacaccg ggccgcgtcg gcgggcgcac caacatgaag gagatcaacg
     2521 tcatcggcac gatgcagctg ttcgccgcgt gccaaaaggc cgagaagctt cgcaaagtcg
     2581 tgatgaagtc ctcgaccgcg atctacggca gcgagccctc ggacccgggc gtgttcagcg
     2641 aggagatgtc accgcgctcg catccgcgga ccggctacgc gaaggactcg gtcgaagtcg
     2701 agaactacgc ccggtcgttc ggccggcgcc gcaccgacgt gtcgctcacg atccttcgct
     2761 tcgccaattt catcggaccg cagatcgaca cgccgctgac ccgctacttc ggtcttccgc
     2821 tcgtgccgac cgcgctgggg ttcgatcctc gcttgcagtt cctccacgaa gacgacgcga
     2881 tcgagatcct gtatcgcgcg accgtggagg accaccccgg aaccttcaac gcggccgggg
     2941 acggggtgct gtacctgtcc caggccatcc ggctcggggg aaaggttccc gtgcccgtgc
     3001 cgcttccctt catcaacatg gtggccgccg cggtgaagcg atccggcatc gtcgacttct
     3061 cgcaggagca ggtgcggcta ttgctctacg gccgcgtggg tgacgtgtcg cggctcaaga
     3121 acgtgttcgg ctacgagccg cagtactcaa cccgcgaggc gttcgccgac ttcgtcgccc
     3181 ggcgcaagct cgcgaagctt ttcacgcccg agcaggccgc acagtgggag cgcgagctct
     3241 atcgcttcct ggcgcgcaga aaggccggtg ccgcatggca gacgcgcgcg tgatcccgat
     3301 ccatctggcc gagtcgccgc gctgcgcggc gcagaccgcg gacggccggc cgtgccgcaa
     3361 ccgggccggg gaaagcggct attgcgcgcg ccacgagccc gaagccgcgg cacgcgaccg
     3421 gcacccgacc ggcgcctcgg cggagccgaa cgcgctccag cagtggctgg aggaggtgct
     3481 cgcattcgtg cggcgtcgaa tgaccggcga ctaccccgtc gacgacatgg ggttcgatcg
     3541 cgatctgacc gagcacgctc tgctgccgct gctgcggccg ctctaccgtt cgtggtggcg
     3601 gatcgatcag atcggcgtcg agaacgtgcc ggccgaaggc cccgtgctgc tcgtgtccaa
     3661 ccattcgggg acgcttccgt tcgacgcgct gatgatgaag ctcgggatct tcgaggagac
     3721 gggccggcac gtgcggctgc tcgccgcgga cctggccttg cagctgccgg tcgtgggtga
     3781 gctcgcccga aagagcggaa acacgctcgc ctgcgaggac gacgcgttgc ggcttttgcg
     3841 tgcgggcgag gtcctgggcg tcttccccga aggattcaag ggcatcggca agcccttccg
     3901 cgagcgctac cgcctgcagc gcttcggtcg cggcggcttc gtggaggtgg ctttgcgggc
     3961 cggcgtgccg atcgttcccg tggcgatcgt cggcgccgag gagatctatc cgatcatcgc
     4021 caacgtcaag ccgctcgcgc ggctgttcaa cttcccgtac ttcccggtga cgcccacgtt
     4081 cccgctgctg ggtccgctcg gcacgcttcc gctgccgtcg aaatggatca tcgagtacgg
     4141 cgagccgatc cacaccgagg gattcggcac cgaggcggcc aacgatccga tgttcgtgtt
     4201 caacctcacc gatcaggttc gcgaggtcat tcagcagacg ctgttcaaga acctgattca
     4261 gcgcaagaac gccttcttct aaacgcagcg cgcccccgcc ggggcgaggg cgcgcgagtg
     4321 cgagccgatc agcgggtcca ggtcacccag acccacgccg acaggttcca gaggtgattg
     4381 ttgtcctgcg tcggccatgc gacacggaac aggcggcggc cgttgtcggt cgccttgtag
     4441 ttgatccggt agaacgacga cgagttcagc cgtgcggtct tgaccgtgac ccactccttg
     4501 ttgatgccgt cgaacatctg cagcagcacg cgggacccgg ggtgacccgg cagcacccgg
     4561 ccgctgaaga ccgcctgctc gccgctgcgc ccgacgccca gatgcacgcc cggccggacc
     4621 tgtgccttga cccagttgct ggtcgacagg tccgaggtga gaaacgcgcg gtaccacgcg
     4681 tgcgcgagcg gctggtcggc ggtgctgaac ttgccgtcgt cgtcgctggt ggtggtggtg
     4741 acgtcctgcc actggttggc cccgacgtac atccgctgca cgcggacctg ccggtttgcc
     4801 gccgtcgtgc cgttcatcgt gaaggtggtt ccccacacgc ccgccgtcgg gctgaccgag
     4861 gtcagcgaga tcggcgtgcc cgctcgcgcg gccggcgcga acgcgacggc cgcgagcgcc
     4921 gtcatgacgc cggcgacggc gagcttcctg cttgctcggt tcatccttgt ccgccctcct
     4981 ggtgatacga catttcgggc gcgagcctac cacggggccg ggctcgctcc cacctcacgg
     5041 gggccaggag ccagaggccg gaagagagaa gaagggcggt ccattcggac cgcccttccg
     5101 atgaagcgtg tagccggtta cttgctgagc gcgaacttgc gagcgccgat cgcgagaccg
     5161 accatcagcg tgccgaccag cagcatcgcg gtgtcgctca cacccgtggt ggggatgcga
     5221 acttccttga caccgaggac gttgccgccg cggacgtccg cgaccgaggc cggaccgtcg
     5281 atcgacaccg cgaacagcgg cgcgcccgac agcagcttcg gagccagacc caggtgcagc
     5341 gac
//
LOCUS       DATLFO010000203         6359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_113897, whole genome shotgun sequence.
ACCESSION   DATLFO010000203 DATLFO010000000
VERSION     DATLFO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113897"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VM841_08395"
     CDS             complement(<1..1717)
                     /locus_tag="VM841_08395"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HVL90237.1"
                     /translation="MRRRLSGALILATLASIMAAPQSVPALGTRTTEAVSVWQHRNSG
                     STTGWDIWWSALGRAGTGPLTWHTNGTPIATALSTLAGDEKNPHVASREAGSIAVWQR
                     STGTGSSATGWDIYFSTLSNSSGTWTAPAAVSTVAGDDYDPNVAIDPAGNAIAVWVHR
                     NTNGTRQIYWSVKPVSGSWTTAAALPSTGGKASLPEITLTAIQSVSGSTIGRYAVATW
                     SDDIGGGVRRQFASRFTGTAWSTPVQIESAATGIGVSIHTVGFAEYLGTDSDPFGAFG
                     RNGVTADAVGNAYIVWSGGPTILNNYSPAVVGAIWNMNSGAWSPMLAPGGSREVGTGG
                     CENPDAAMTSGAGDFVSVYSFNGYIEDTRRVGGSFTYEQYSYGSSLDDQRPSVAAISD
                     TQVVSVNWGAPQGSGSQIAWSVGTFTGGGNITWTSAATLALSGEDMFPEVASGFIPPL
                     YTGDAYTLDLRVEGTPQNLGIHTLAGARTMDGTAEGLLPKVVLPLGLGEISGAHNTAS
                     AGGGSASATSVIERVSLLNGAIQAEAIRTQANASLGPPPSTNSTGTRIGRLVVGGQTI
                     DVPATG"
     gene            complement(1803..3152)
                     /locus_tag="VM841_08400"
     CDS             complement(1803..3152)
                     /locus_tag="VM841_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVL90238.1"
                     /translation="MTYTEEPGAGRWRTWIVEPQDIAVAPPPALGGVPAAVELADVKL
                     AVATRGAATLEAIRRWGAGAATAPWTALFLDLTKRYSAYPERNPPRISRQIAMLHAAM
                     FDALVATWNAKYCHLRPPPSKVDPTIAVHGPAADAPSYPSEHAAVAGAAAVLLASFFP
                     EEPEGSFDALQWEAGMSRVSAGANYRSDVTAGIVLGRSVAKLVLDARATDGWNASWDG
                     SPGLGGTCGWSPVSPVAPVEPMWGKVRPWLMSTGSQLRPGAPPACDSPAYLAAARDVY
                     EASLTLTQRQRDIATSWAGGPGTETPPGMNARIALETASAAGLSTMRHARVMAHVGAA
                     LADAAIAAWDAKFTYGWDRPIQTIRRLWDPQWTPLLPTPPFPGYVSGHATFSGATARV
                     LGFFFPSQSGVFRALATEAALSRFYGGIHARYDNDAGLTVGEAVAELAVARAGQDRA"
     gene            3451..4011
                     /locus_tag="VM841_08405"
     CDS             3451..4011
                     /locus_tag="VM841_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90239.1"
                     /translation="MYVPKRVVLLWILLFALVRPVGEAGAAGGVFIGYGQHTEGPTKV
                     VDLPGRKLVTGPLTATFSVGPDHAVCAVGEAGGEAGAFNMLVYATEITSSRLQPNGKT
                     WRFAGTARSVTMVGSVVVEDALTAFTAEAHDAGPGVAGDFFHITINTSLFPHTTFGKD
                     LDGNALPLFSGDVAIWTELANGQNYS"
     gene            4134..>6359
                     /locus_tag="VM841_08410"
     CDS             4134..>6359
                     /locus_tag="VM841_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL90240.1"
                     /translation="MTEIESGSLHGRATEERRIASTLEEAGAGRRRGAVLLGGPGMGK
                     SVALRRAAVLAAEQGFFTVSVRVPGGIPLPPHVPVPEIVAQLAESGSASGELAGDGGA
                     SFAALTGALRERSEGHRLAILIDDFHWAPAEGANLLLSALRAIDDPIAIFLAMRPATR
                     VHGETLPIPESTGDLPFEVIELLPLESDAVESIAKDMLGGDVVPSLVRALEQETQGNP
                     LHIVEAIRDGRAEGWIEHTAGFWAAREGFHPRTMLDSIRAQLERLPRPAFRVAGFLAV
                     LGRPSRLEEIAHAMDGGSPRGPEEALDALDVLLSTGIATEDAPRTYRLSHPLHASALI
                     DLIGPARRAAEHQRALAILRGQPCPASELAYHATRAATTPDDLDRLVAAAGRESEALG
                     APAAAAGWYRVLVDMLAPGNPQRATALESLARTTAQYDPASSIERYTDALREATGHER
                     ARLLTSRARAMQRTGRFDEALADLREALPGAAEADVFAIEAGMGVLELLTGDRAAAEA
                     RLAALVERSRQTRNHPRALYHLAAARLFAGDVASFSALSREAMEATEDVHLARSALSN
                     VAWGLILQGRFADAEALILPAIEEIVAAGDRGALFPLMTNASLLMSWKGEIGAAADFA
                     LRSRSLADAIGNPADRAAALMCMMLPLLESGTPALARPLAEEIDRVLEQISELREVGL
                     TYLLVGDAYLALGDLDAAHARCRRAERFLDLDSLIWAEGVARLDAEIAIASGEPSRAA
                     EI"
BASE COUNT          948 a         2277 c         2155 g          979 t
ORIGIN      
        1 tcccggtcgc gggaacgtcg atggtctggc cgccgaccac aagccgtccg attcgggttc
       61 cggtcgaatt ggtcgacggt ggcggtccga gcgaggcgtt ggcttgcgta cggatggcct
      121 cggcctgaat cgcgccgttg agcagcgaca cgcgttcgat caccgacgtg gccgaggcgc
      181 tgccaccgcc cgcgctcgca gtgttgtgcg ctccggagat ctctcccagg ccgagcggca
      241 agaccacctt cggcagcaga ccctccgcgg ttccgtccat cgtccgcgcg ccggcgagcg
      301 tgtgaatgcc gagattctgc ggcgttccct ccacccgcaa gtcgagcgtg tacgcgtcgc
      361 cggtgtagag cggcggaatg aatccggatg cgacctcggg gaacatgtcc tcacccgaga
      421 gcgcaagcgt cgcggcggac gtccaggtga tgtttccccc gccggtgaac gtgccgaccg
      481 accaagcgat ctggctgccc gatccctgcg gggctcccca gttgaccgac acgacctgcg
      541 tgtcggagat cgcagccacc gatggccgct ggtcgtcgag cgaggaaccg taggaatact
      601 gctcataggt gaaggatccg ccgacgcgac gggtgtcctc gatgtatccg ttgaagctgt
      661 agacgctgac gaaatcgccg gccccgctgg tcattgccgc gtcgggattc tcgcatccgc
      721 ccgtgccgac ttctctcgaa ccgcccggcg cgagcatcgg gctccacgca ccggagttca
      781 tgttccagat ggcaccgacc accgccgggg agtagttgtt gaggatcgtc ggacctcccg
      841 accagacgat gtacgcgttg ccgacggcgt cggccgtgac cccgttgcgt ccgaaggcgc
      901 cgaacgggtc cgaatccgtt cccaggtact cggcgaagcc gaccgtgtgg atcgagacgc
      961 cgattcccgt cgccgcggac tcgatctgaa cgggcgtgct ccacgccgtt ccggtgaaac
     1021 gtgatgcgaa ctgccgccgc actcccccgc cgatgtcgtc ggaccaggtc gcgaccgcgt
     1081 accgtccgat cgtgctgccg gacaccgact ggatggccgt caaggtgatc tcgggcagcg
     1141 acgccttgcc gccggtcgag ggaagcgccg ccgcggtcgt ccacgagccg ctcaccggct
     1201 tgaccgacca atagatctga cgcgtgccgt tggtgttgcg atgcacccag acggcgatcg
     1261 cgttgccggc gggatcgatg gcgacgttcg gatcgtagtc gtcgcccgcg acggtcgaaa
     1321 ccgcggcggg cgccgtccag gtgccggagc tgttgctgag cgtcgagaag tagatgtccc
     1381 acccggtcgc ggaggatccc gtgccggtcg agcgctgcca cacggcgatg cttcccgcct
     1441 cgcggctcgc gacgtgcggg ttcttctcgt ccccggccag cgtggaaagg gccgttgcga
     1501 tcggggttcc gttcgtgtgc caggtcagcg gaccggtgcc ggcgcggccc agggccgacc
     1561 accagatgtc ccagcccgtc gtggacccgc tgttgcgatg ctgccagacc gagacggcct
     1621 cggtcgtgcg cgtgccgagc gccggaacgc tctgcggtgc tgccatgatg ctcgcgagcg
     1681 tggccaagat cagcgcaccg ctaagacggc gtcgcatagt tgcctccccc caaatgcatc
     1741 ccgaacgatg cttttcctaa aatatgccta atccgacagg tcagataatt ctgacatatt
     1801 gattatgctc gatcttgccc tgctcgcgct acggccagct ccgcgacggc ctcccccacg
     1861 gtgagcccgg cgtcgttgtc gtaccgggcg tgaatgcccc cgtagaagcg cgacagcgcc
     1921 gcctcggtcg ccagcgcgcg gaataccccg gactgcgacg ggaagaaaaa gccgagcacc
     1981 cgtgcggtcg cgcccgagaa cgtcgcgtga cccgacacgt agcccggaaa cgggggtgtc
     2041 ggcagaagcg gcgtccactg cggatcccac agacgccgga tggtctggat cggccggtcc
     2101 cacccgtagg tgaacttggc gtcccacgcg gcgatcgcgg cgtccgcgag cgccgccccg
     2161 acgtgcgcca tcactcgcgc atgccgcatc gtgctcagcc cggcggcgct cgccgtctcc
     2221 agcgcgattc gcgcgttcat gccgggcggg gtctcggtcc ccgggccgcc ggcccagctg
     2281 gttgcgatgt cccgctgccg ctgcgtcagc gtcaacgacg cttcgtagac gtcccgggcg
     2341 gccgccagat acgccggcga atcgcacgcc ggaggcgccc ccggccgcag ctgggatccg
     2401 gtgctcatca gccatgggcg aaccttgccc cacatcggct ccaccggagc gaccggcgag
     2461 accggcgacc agccacacgt tcctcccagg cctggcgaac cgtcccagga cgcgttccat
     2521 ccgtcggtgg cccgagcgtc cagcaccagc ttggcgaccg aacggccgag cacgatcccg
     2581 gccgtgacgt cggaccggta gttcgcgccc gccgacacgc gggacatgcc cgcctcccac
     2641 tgcaaggcgt cgaagctgcc ttcgggctcc tccggaaaga aggaagccag aagcaccgcg
     2701 gccgcgccgg ccacggccgc gtgctcgctc ggatacgacg gagcgtcggc cgccgggccg
     2761 tgcaccgcga tggtgggatc gaccttggac ggcggcggac gaagatggca gtacttggcg
     2821 ttccaggtcg ccacgagcgc gtcgaacatg gccgcgtgaa gcatcgcgat ctgacgcgag
     2881 atccgcggcg ggttgcgctc gggataggcg gagtatctct tcgtgaggtc caggaatagc
     2941 gcggtccagg gcgccgtcgc cgcaccggcg ccccagcgcc ggatcgcctc gagcgtcgcg
     3001 gcgccccgcg tcgcgacggc gagcttgacg tcggccagct cgaccgccgc gggaacgccg
     3061 ccgagcgccg gcggcggagc aacggcgatg tcttggggct ccacgatcca ggttcgccag
     3121 cggccggccc cgggctcctc ggtgtaggtc acggcgcggt gctggctcgc cgcgaccgca
     3181 acggcgtcgc gcggctcgca gagcgccctc gcaccggcgg gagccgttcc cgacaacgcc
     3241 gaaaccgccg cagccaaagc ggcggcgaga gcgatagccg gatgtctcct cacgtgtccc
     3301 cccgggcgac aattcggcga ccgttgtgcc tgctcctgcg gagcggtgga aaatcctgat
     3361 tgatcccact agcccggcag gaattcaatt gcggaacccg aacctgacgt gacgggctca
     3421 atcggggctc gaatcggggt ggaggccgtc atgtacgttc ccaagcgagt cgtcctgctg
     3481 tggatcctgc ttttcgcatt ggtccggccg gtcggcgagg cgggcgccgc gggaggcgtc
     3541 ttcatcgggt acggccagca caccgaaggt ccgaccaagg tcgtggacct ccccgggcgc
     3601 aagctcgtga ccggaccgct gacggccacc ttctcggtcg gacccgacca tgccgtctgc
     3661 gccgtcggcg aggcgggcgg cgaggccggc gcgttcaaca tgctcgtcta cgcgaccgag
     3721 atcacctcga gccggctcca gcccaacggc aaaacgtggc ggttcgcggg aaccgcgcgc
     3781 tcggtgacca tggtcggatc ggtcgtggtc gaagacgcgc tcaccgcctt caccgcggaa
     3841 gcgcacgatg ccggtcccgg cgtggcggga gacttcttcc acatcacgat caacacgtcc
     3901 ctgtttccgc acaccacgtt cggcaaagat ctcgacggca acgctctgcc gctcttctcg
     3961 ggcgacgtgg ccatctggac cgagctcgcc aacggccaga actacagctg atcgcggcgc
     4021 cggccgttcc cgcagtcttc gcacacgggt gaaatggccg gccccgccgc gcgtctgttc
     4081 atgcgacagt ggctagactc gcacggctcg gatcggtgga tccctgcatg ctcatgaccg
     4141 agatcgaatc cggctctctt cacggtcgcg cgaccgaaga gcgacgcata gcctcgaccc
     4201 tcgaagaggc cggggccggg cgccgccgcg gcgccgtcct cctcggcggg ccgggcatgg
     4261 gcaaatccgt ggcgctgcgc cgcgctgccg tgctcgcggc cgagcaaggc ttcttcaccg
     4321 tgtcggtccg cgtccccggg ggcattccgc tgccgccgca cgttccggtc cccgagatcg
     4381 tcgcccagct cgccgaatcg gggagcgctt ccggcgagct cgcaggcgac gggggcgcgt
     4441 cgttcgcggc cctcacgggc gccttgcggg agcgttccga aggtcatcgc cttgccatcc
     4501 tcatcgacga ctttcactgg gcacctgccg aaggcgcgaa cctgctgctg tcggcgctgc
     4561 gggcgatcga cgatccgatc gcgatcttcc ttgcgatgcg tcccgcgacg cgcgtgcatg
     4621 gggagacgct gccgatcccc gagtcaaccg gcgatctgcc gttcgaggtg atcgagctgc
     4681 tccccctgga atcggatgcc gtcgaatcga tcgccaagga catgctcggc ggcgacgtgg
     4741 tcccctcgct cgtgcgcgcc ctcgagcaag agacgcaagg caatccgctt cacatcgtcg
     4801 aggccatccg cgacggccgg gccgagggat ggatcgagca cacggccggc ttctgggccg
     4861 cgcgcgaagg gtttcatccg cggaccatgc tcgactcgat tcgcgcgcag ctcgaacggc
     4921 tcccccgacc ggcgttccgg gtggccggct tcctcgcggt cctcggacgc ccgtcccgtc
     4981 tcgaagagat cgcgcacgcc atggacggtg gatcgccccg cggtcctgaa gaggcgctcg
     5041 atgcgctcga cgttcttctg tccaccggca tcgcgaccga ggacgcgccg cgaacctacc
     5101 ggctcagcca cccgctccac gcaagcgcgt tgatcgacct gatcgggcct gcgcggcgag
     5161 cggctgagca ccaaagagcc ctcgccatcc ttcgcggcca gccatgcccg gcatccgagc
     5221 tggcctatca cgcgacgcgc gcggcgacga ctcccgacga cctcgatcgc ctcgtcgccg
     5281 ccgccgggcg cgagtccgaa gccctaggag cacccgccgc cgcggccggc tggtaccggg
     5341 tgctggtcga catgctcgct cccggcaacc cgcaacgcgc gacggcactg gagtcgctcg
     5401 cccggacgac ggcgcagtac gacccggcga gctcgatcga gcgctatacc gacgccctgc
     5461 gcgaagcgac cggccacgaa cgcgctcgtc tgctgaccag tcgcgcacgc gcgatgcagc
     5521 gcaccggccg cttcgacgag gccctcgccg acttgcgcga ggccctgccc ggcgccgccg
     5581 aggccgacgt gttcgcgatc gaggccggga tgggggtgct ggagctgctc acgggcgacc
     5641 gcgccgcggc cgaggcgcgg ctcgccgctc tcgtcgagcg gtcgcgacaa acgcgcaatc
     5701 atccgcgtgc gctgtatcac ctggcagcgg cccggctgtt cgccggcgac gtcgcgtcgt
     5761 tctcggcgct gtcgcgcgag gcgatggagg cgaccgagga cgttcatctg gcccgctcgg
     5821 cgctgagcaa cgtcgcgtgg ggactgatcc tgcaaggacg gttcgccgac gccgaggcat
     5881 tgattcttcc cgcgatcgag gagatcgtgg ccgccggcga ccgcggcgcg ctgtttcccc
     5941 tgatgaccaa cgcatccttg ctgatgtcgt ggaagggcga gatcggcgcg gccgcggact
     6001 tcgcgctgcg aagccgctcg ctcgccgacg ccatcggaaa cccggccgac cgcgcggccg
     6061 cgctcatgtg catgatgctt ccgctcctgg agtctggcac gcccgcgctg gcccgaccgc
     6121 tggccgagga gatcgaccgg gtgctcgagc agatcagcga gctccgcgag gtcggcctga
     6181 cctatctgct cgtgggcgac gcgtacctcg cgctcggaga cctcgatgcc gcccacgccc
     6241 gctgtcgccg ggccgagcgt tttctcgatc tggattcgct gatctgggcc gagggcgtgg
     6301 cgcgcctgga cgccgagatc gcgatcgcct ccggcgagcc gtcccgcgcc gcggagatc
//
LOCUS       DATLFO010000204         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_106828, whole genome shotgun sequence.
ACCESSION   DATLFO010000204 DATLFO010000000
VERSION     DATLFO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106828"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VM841_08415"
     CDS             complement(<1..1078)
                     /locus_tag="VM841_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HVL90241.1"
                     /translation="MTGHAATSDRETPAAERERRHRSRRRRRRRTRIRAAVRAAVVAI
                     AGGLAALGAGFAWETYSAYASLQRAEPMLASARAAARDAKPADAGRALREAHPHVRAA
                     VSRASGPFTGIVAAVPVAGRSARGVRAGAEAAELAVRAGLGATAAAEAFPTRDGGIAS
                     FGFVNGRIPVEAWRDAAAPLEAADELARRALARVHAADRSLILPPLARALSRLDEDLT
                     ELRKATGQAASLATVIPALAGGEGRKRYLLAFQNPAELRATGGLLGGVALLDANAGVI
                     EITDTLPDNDLRPLEQAPVKMPDWYVTRYERLRGMTIWQNVNMEPHFPVSAPLIAGFY
                     RATTGTEVDGVIALDPFALSAMLEA"
     gene            complement(1417..2328)
                     /locus_tag="VM841_08420"
     CDS             complement(1417..2328)
                     /locus_tag="VM841_08420"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVL90242.1"
                     /translation="MRGLRRTWLAASLALASLALLTAAPTHAGSAHDRARQIDGQPNS
                     FYWAVLVGIDDYQGNTPDLTGSVNDVRVMRDHLLSLGWRNDHIYVVTDKAATRDGILR
                     AIRWLASKTNNRSAAIFHFSGHEWPMRTTADGDDEARDVAIRTHENKFIIDGDLGREM
                     DRVVAHRFWIDMSLCRAGGFTDRGMIKPGRVLTFSSPESQLSYEDPSVRHSVFSYYAI
                     AQGMRSGHADKNGDGQVSVEEAFWYSRMPVINRTDGRQHPEIIDKLAGDFSITPPAPK
                     ASQEPRPTPSPTPSRTCVAGICLPAKV"
     gene            complement(2366..3385)
                     /locus_tag="VM841_08425"
     CDS             complement(2366..3385)
                     /locus_tag="VM841_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HVL90243.1"
                     /translation="MTLRLALELPYPEAQNRQDMIELARAAEANGFDSIWASELYSYD
                     AFTTLTHLACHTSTIKLGTNIANIYARTPALLAGTAASLDQLSDGRFILGIGVSGPQV
                     VEGWHGVPYDTPLKRTSETIDILRTVMAGGRLNYEGEVFNVTMGLKLINKGQRPDIPI
                     YVAAIGPKNIELTAEKADGLLPTFFAASHADEIFKPHVDAGLRKAGKDPEDFAVMPLV
                     NAFVGDVKDGINACKMLAGFYIGGMGSKKKNFYNDLMKRYGYEELAEKIRDTFLGGDK
                     QTAIEMVPDDLVDDMSLIGDEARLRDRMKAFEAAGATGVVVVPWAADAQGRTTVLETL
                     ARANS"
     gene            complement(3435..3902)
                     /locus_tag="VM841_08430"
     CDS             complement(3435..3902)
                     /locus_tag="VM841_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HVL90244.1"
                     /translation="MSEPREIICVCYANICRSPMAEAILQQALDLRFGAGTFTVTSAG
                     IQARPGLPADPGSVAALGKRGLDASRHQSSLLTKERAERAWRIYAMEEYQAQWIRSLI
                     GSAGTIHVWGEEIPDPIGGSRRRFEGAAALIERSLPSILDDIAAALAAEGSRA"
BASE COUNT          561 a         1341 c         1442 g          665 t
ORIGIN      
        1 tcgcctcgag catggccgac agcgcgaacg ggtcgagcgc gatgacaccg tccacttcgg
       61 ttccggtcgt ggcgcggtag aagccggcga tcagcggcgc gctcaccggg aaatgcggct
      121 ccatgttgac gttctgccag atcgtcatgc cgcgaagccg ctcgtatcgc gtgacgtacc
      181 agtcgggcat cttcaccggc gcctgctcga gcgggcgaag gtcgttgtcg ggcagcgtgt
      241 cggtgatctc gatcacgccg gcgttcgcgt ccaggagcgc gacgccgccg agcagcccgc
      301 cggtcgcgcg cagctccgcc ggattctgga acgccagcag atagcgcttg cggccttcgc
      361 cgcccgcgag cgcgggaatg acggtcgcga gcgaggcggc ctgtccggtc gccttgcgca
      421 gctcggtcag gtcctcgtcg agacgcgaca gtgcccgtgc gagcggcggc aggatcagcg
      481 aacgatcggc cgcgtggacg cgagccagcg cgcggcgcgc gagctcgtcg gcggcttcga
      541 gcggggccgc cgcgtcgcgc cacgcctcga ccgggatgcg gccgttgacg aatccgaacg
      601 aggcgatgcc gccgtcacgc gtcgggaacg cctcggccgc cgcggtcgcg cccaggcccg
      661 cgcgcaccgc aagctccgcc gcctcggcgc cggcccgcac gccacgcgcg ctgcgtcccg
      721 cgaccggcac ggccgccacg atgccggtga acggtcccga ggcgcgcgag acggcggcgc
      781 gcacgtgcgg gtgcgcctcg cgcagcgcgc ggccggcgtc ggccggtttg gcgtcgcgcg
      841 cggccgcgcg tgccgaagcc agcatcggct cggcccgctg cagcgacgcg tacgccgagt
      901 aggtctccca cgcgaagccc gcgccgagcg ccgcgaggcc tcccgcgatg gcgacgaccg
      961 cggcgcggac cgcggcgcgg atgcgcgtgc ggcgacgccg gcgacgccgg gaacggtggc
     1021 gccgctcgcg ctccgccgcg ggcgtctcgc gatccgaggt cgccgcatgg ccggtcatgg
     1081 cctcgatcgt accgttcacc tccttagtgc ccgaatgtgg gggtttgctc gatttctgca
     1141 ggtcagcgcg gtaaagccct cttcagcgga acgctaaagt ttcccgattt ctgtgccgat
     1201 gttgccaatg aggggaggaa acgggtcggc gccccgcccc cgccgggcac cccaaccgtc
     1261 ggcccgttcc tcctaccgaa gattgcagaa tccctcttct gcgcgcaggc cgccattccc
     1321 caccgggcgg cctgcacctt ttccggcgcc ttcgccggtc agcccttggc aggcaggccg
     1381 atgccggtca gctctgcgca ggcaggccga tgccggtcag accttcgcag gcaagcagat
     1441 gccggcgacg caggtgcgcg acggtgtcgg gctgggcgtc gggcgcggct cttgcgacgc
     1501 cttgggcgcg ggcggcgtga tcgagaagtc gcccgcgagc ttgtcgatga tctcgggatg
     1561 ctggcggccg tcggtgcggt tgatcaccgg catgcgcgaa taccagaagg cttcctcgac
     1621 gctgacctgg ccgtcgccgt tcttgtcggc gtggccgctg cgcatgcctt gcgcgatcgc
     1681 gtagtagctg aagacgctgt ggcgcacgct cgggtcctcg tagctgagct ggctttccgg
     1741 gctcgagaag gtgagcacgc gcccgggctt gatcatgccg cggtcggtga agccgcccgc
     1801 gcggcacagc gacatgtcga tccagaagcg gtgcgcgacg acgcggtcca tctcgcggcc
     1861 gagatcgccg tcgatgatga acttgttctc gtgcgtgcgg atggcgacgt cgcgcgcctc
     1921 gtcgtcgccg tcggcggtgg tgcgcatcgg ccactcatgg ccgctgaaat ggaagatcgc
     1981 ggccgagcgg ttgttggtct tggaggccag ccatcggatc gcgcgcagga tgccgtcgcg
     2041 ggtggcggcc ttgtcggtga cgacgtagat atggtcgttg cgccatccga gcgagagcag
     2101 atggtcgcgc atcacgcgca cgtcgttgac cgagcccgtc aggtccgggg tgttgccctg
     2161 gtagtcgtcg atgcccacca gcacggccca gtagaaggag ttgggctgtc cgtcgatctg
     2221 gcgcgcccgg tcgtgggccg agcccgcatg ggtgggggcg gcggtcagca aagcgagcga
     2281 ggcaagcgcg agcgaagccg cgagccaggt ccgacgcaat cctctcacca tgaggggttt
     2341 tcgcggcttg cccgaccgac tccttctaag agttggcgcg agcgagcgtt tccaacacgg
     2401 ttgtcctgcc ttgggcgtct gcggcccagg ggacgaccac gaccccggtc gcgccggcgg
     2461 cctcgaaggc cttcatccgg tcgcgcagcc tcgcctcgtc gccgatcaag ctcatgtcgt
     2521 ccaccagatc gtcgggcacc atctcgatgg ccgtctgctt gtcgccgccg aggaaggtgt
     2581 cgcggatctt ctcggccagc tcctcgtagc cgtagcgctt catcaggtcg ttgtagaagt
     2641 tctttttctt gctgcccatg ccgccgatgt agaaaccggc gagcatcttg catgcgttga
     2701 tcccgtcctt gacgtcgccg acgaaggcgt tgacgagcgg catgaccgcg aagtcctcgg
     2761 gatccttgcc cgccttgcgc aggcccgcgt cgacgtgggg cttgaagatc tcatcggcgt
     2821 gcgaggcggc gaagaaggtg ggcagcagcc cgtcggcttt ctcggcggtc agctcgatgt
     2881 tcttcgggcc gatcgccgcg acgtagatcg ggatgtcggg ccgctggcct ttgttgatca
     2941 gcttcaggcc catcgtgacg ttgaagacct cgccctcgta gttgagccgg ccgcccgcca
     3001 tcacggtgcg caggatgtcg atcgtctcgc tcgtgcgctt gagcggcgtg tcgtagggca
     3061 cgccgtgcca gccttcgacc acctgcgggc ccgacacgcc gatgcccagg atgaaacggc
     3121 cgtcgctgag ctgatccagg ctcgcggccg tgccggccag cagcgcaggc gtacgcgcat
     3181 agatgttggc gatgttggtg ccgagcttga tcgtcgaggt gtggcacgcc aggtgcgtga
     3241 gcgtcgtgaa agcgtcgtag gagtagagct cggacgccca gatcgagtcg aagccgttcg
     3301 cttcggcggc gcgcgccagc tcgatcatgt cctgccggtt ctgcgcctcc ggataaggaa
     3361 gctcgagtgc aagtcgaagt gtcatcgtgg gccctcccca ggggtcgaag cgagcctatc
     3421 gcaggagcgt cgcctcaggc gcgcgagcct tcggccgcga gcgccgccgc gatgtcgtcg
     3481 aggatcgagg gcagcgaacg ctcgatcagc gcggccgcgc cttcgaagcg ccgccgcgag
     3541 ccgccgatcg ggtcggggat ctcctcgccc cagacgtgga tcgtgccggc cgagccgatc
     3601 aaggagcgga tccactgggc ctggtactcc tccatcgcgt agatgcgcca ggcccgctcg
     3661 gcccgttcct tggtgagcag gctggattgg tgccgtgagg cgtccaggcc gcgcttgccg
     3721 agggcggcga cgctgcccgg gtcggccggc agccccggcc gggcctggat gcccgccgag
     3781 gtgaccgtga acgtgccggc cccgaagcga aggtcgaggg cctgctgcag gatcgcctcg
     3841 gccatggggg agcggcagat gttcgcgtag cagacgcaga tgatctcgcg tggttcgctc
     3901 atgagatagt cgagtctaga gccgatcgcg ccggtgcgcg gccgttcggt ccggaaatac
     3961 agaaatgggg cccgtcgtcg ggccccattt cgaaaagtgc gtgtgtgtt
//
LOCUS       DATLFO010000205         8039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_133600, whole genome shotgun sequence.
ACCESSION   DATLFO010000205 DATLFO010000000
VERSION     DATLFO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133600"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VM841_08435"
     CDS             <1..856
                     /locus_tag="VM841_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90245.1"
                     /translation="LESAPWRPATTGELLPNLRVTRLWEFGFAAPANPGNGLFPPDDL
                     NPPLSVAGFEPVSCSVDEQLGFDNPEQPAQRCLRYSFGLANAGEGNFDVRYTNDRSGN
                     TTAMTQCVQQADGSSPRARPAGTGAFHQTHGHFHYSNVIFHDVWRITDPSIWAREAIG
                     AGKKIGYSPADQGIADWFDFTQMAPGTSGSAGNCAPGTNSRLGMSVGWGDAYRYQRPG
                     NFVEFGNAGDGLYVVRTIADPENVVEESDESDNTAYALIRIVGTEINVLETGQGNGPW
                     DSGKVIYE"
     gene            853..1242
                     /locus_tag="VM841_08440"
     CDS             853..1242
                     /locus_tag="VM841_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90246.1"
                     /translation="MTNARITAVAALMLALAPMSSRAATEIVAVPGSFVSTYATPVVV
                     WTAGMPAPKFVNTDIQAHDVVSDATRAPGSAPWCPAGGPRCPMFFAPLLGLGGMTDVI
                     GLPHTPPGVYPFACSPHPWMTGTLVVV"
     gene            complement(1309..3654)
                     /locus_tag="VM841_08445"
     CDS             complement(1309..3654)
                     /locus_tag="VM841_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL90247.1"
                     /translation="MSNGLLIKRGSMVRAALVLALLLGSLTGKSASAFPAGAHVAGSV
                     EQVYATGLPGGASVALIAPDGSVVETRTVTAQGGVLFRTVTPGDGYRVRLGSGDSDPV
                     TVRDATAAPWDPSIYHQSMPSSGYGYLTTRDGTKLAYTVHPPTSPAGQAGLPHGSPVP
                     ATGTDYLPPYPTLIEYSGYSNAKPDGPQSGIAVLANLMGFAVVNVNMRGMGCSGGAFD
                     FFETMQSLDGYDIVEIVARQPWVKHNKVGMMGISYGGISQLFTAQTQPPSLAAITPLS
                     VIDSTATTLYPGGNRNTGFAVEWALARQRDARPASATTGQRWAWDRIQAGDTICAANQ
                     ALHGEAADLLAAIEANTHYDAAVADPLSPNTFVHKINVPTFLVCQWQDEQTGGHCPEL
                     VNNFTGTDKKWFTFTNGPHIDSLAPEIYTRWFDFLMLYVAKEAPILKQALVRASAPVV
                     FNAAMGIPPQDSALLPVDPVQLMPTYELALAEFEKAPMVRVLFDNGAGASPEGRTIAG
                     NPYAAFERSFSSYPAPGTVAKSWYFGANGTLRDSLPSATNVDRYTSDPNALPLTNYAG
                     GTGGGGLWGNASQWKWNWKPNPAGTAVSYVTAPLANDTTVLGSGAVYVWVRSAVPDVD
                     LQATVSEVRPDGNETFVQNGYMRGSMRKLSTDSNNMFKRPGTLLAPVPTFLAADVSPM
                     PSDEFVRVAVPMYHQGHVYRAGSRIRVTIAGPNGAQPVWSFDNPVPGGVIDISSSPAQ
                     PSSLVLPVVPGMNAGAPLPPCPSLRNQPCRPYVALVNQTIS"
     gene            3874..7524
                     /locus_tag="VM841_08450"
     CDS             3874..7524
                     /locus_tag="VM841_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90248.1"
                     /translation="MRRVRVKLSTLLALVVGLSPLALFAADEIVASSDLARVVIELSD
                     RPLATYEDAIPRLRGVLGARTAGGHLDVAAAASRGYLRYLDERQDAFERQLAAASRGA
                     EIHWRYRIAFNGITALLRRDTIPAVRALPGVVAVTETYELEPELDQSRDKLDLPAMWS
                     GFKDSPLGPGDGLRVALLDSGVNPHHPFFSPAGYTAPPGYPKSERVSGGTRTPLPAPL
                     YTSNKVIVGNVYTPPGGTATPWGPGSTHGTHVAGIMAGVEGTYPYTLGGKTWDLTFSG
                     VLPGAYIMSYLPAGDSAEFLAAIEDIVADEADALNISLGHSRWLTGDPVHDPLRKALD
                     AAVAAGTVVAASAGNAGSNGDSTVTGSWKLSDKVITVASNSHGRMFANKLSVTGSATP
                     PARLRDLIAVPAGGTTPPFAVEVSGQYVVAPGGNGPDAGLACSELPPEEVAGKIVLVG
                     RGVCTFEAKKDNVRNAGGIAMIVHNNGPDLPTAMSFTTPAIPAAMISQADGHAMITWA
                     KKDGAPTLAVAPGTERVTTAPADVVSSFSSRGPAPIMRIKPDISAPGFSVLSSVVEGT
                     TGAVGAPLFEMLSGTSMSTPHVTALAAATKGLHPTWTPMQIKSALMNTAHTSMYLDAT
                     RSTPASVRDRGAGRVQPARLLNPALTFDPPSVSLGMVRAGETAATTVTATDMRGRGPS
                     KSWAVSVRAVKGHDRVVVTPTPSPATAPGGAASIAIRVSTAGAPAGDYEGFVEIRSGS
                     ERYTLPYWVRVQPAAAKDVLLIDWDRNLPEDLSGPYKDALRKLGLTFDVFDGGTSAAA
                     NAAPGPTLLQLQAYRTVILFTGNNSASWSNAHIGGSFALQGYLLGGGRLIMAGQDLQS
                     QIVNNQNQGSDSMLTSMGGWISGMTAVETPAACQVVISDSNFYRDATPIEDLTTVLEL
                     FGGTVDVSSTASGGPGAQTRPDAGRAVTADDDVNTCLRVYNADAIGPHARVLGSYVTT
                     RRNGIDVPRAENAAATGVAPDTTLERRSRLVPWTAAVLHVGIEGVNEGQGRLSRAATL
                     GRLYDFANDTVTVEVSQTARDGASVDLVAKASSTRDAKIVKYRWDFGDGSGIKETTTN
                     RVRQTYAKGVYTVRVEAVTELTRSGVGVATIDTRTSVLNNRTPRKPAPAKDLPATEYD
                     GPIPETDARRGIEQPRSVLAAPKPVLETIRGPVDAQPDHTSWILLAFTIWAATAAPLT
                     ARFAGAGRRRTG"
BASE COUNT         1231 a         2850 c         2754 g         1204 t
ORIGIN      
        1 gctggagagt gccccctggc gacccgcgac caccggcgag ctgctgccga accttcgcgt
       61 cacgcgcctg tgggagttcg gcttcgccgc tccggcgaac ccgggcaacg gcctgttccc
      121 gcccgacgac ctgaacccgc cgctgtcggt cgccggcttc gagccggtgt cgtgcagcgt
      181 cgacgagcag ctcggcttcg acaaccccga gcagcctgcg cagcgatgcc tgcgctactc
      241 gttcgggctc gccaacgcgg gtgagggcaa cttcgacgta cgctacacca acgaccgctc
      301 gggcaacacc actgcgatga cgcagtgcgt gcagcaggcc gacggaagct cgccgcgcgc
      361 acgccccgcc gggaccggcg cgttccatca aacccacggg cacttccact acagcaacgt
      421 catcttccac gacgtgtggc ggatcaccga cccctccatc tgggcgcgcg aggcgatcgg
      481 cgccggtaag aagatcggct atagcccggc cgaccaaggc atcgccgact ggttcgactt
      541 cacgcagatg gcgcccggca ccagcggcag cgccggcaac tgcgcgcccg gcacgaacag
      601 ccgcctgggc atgagcgtgg gctggggcga cgcgtaccgc tatcagcgtc ccggcaactt
      661 cgtcgagttc ggcaacgcgg gcgatggcct gtacgtggtc cgaacgatcg ccgatcccga
      721 gaacgtggtc gaagagtccg acgagtccga caacaccgcc tacgcactca tccgcatcgt
      781 cggaaccgag atcaacgtgc tcgagaccgg ccaaggcaac ggcccgtggg attccggaaa
      841 ggtgatctac gaatgacgaa tgctcgcatc actgccgtcg ccgccctgat gctcgccctg
      901 gcaccgatgt cctcgcgcgc cgcgaccgag atcgtcgcgg tcccgggctc gttcgtctcg
      961 acctacgcca cgcccgtggt ggtgtggacc gcgggcatgc cggcgccgaa gttcgtcaac
     1021 accgacatcc aggcccacga cgtggtgtcg gacgcgacgc gcgcgccggg cagcgcgccg
     1081 tggtgtccgg ccggcggtcc gcgctgcccg atgttcttcg caccgctgct cggcctgggc
     1141 ggaatgacgg acgtgatcgg gctgccgcac acaccgcccg gggtttatcc gttcgcgtgc
     1201 agcccgcatc cgtggatgac gggaacgctg gtcgtggtgt gaggcggatc aggccggcgg
     1261 gttgacggtg aatccgccgg cgcccgcgcc caacaagcag cggccggact acgagatggt
     1321 ctggttcacc aacgcgacgt acgggcggca gggctggttg cgaaggctcg ggcacggcgg
     1381 cagcggcgct ccggcgttca tgcccggcac gaccggcagc accagcgatg agggctgcgc
     1441 cggcgacgac gagatgtcga tcacgccgcc gggcaccggg ttgtcgaacg accagaccgg
     1501 ctgcgcgccg ttgggtcccg cgatcgtcac gcggattcgc gagccggcac ggtacacgtg
     1561 tccctggtgg tacatcggga ccgcgacccg cacgaactcg tcgctcggca tcggcgagac
     1621 gtcggcggcc aggaacgtgg gcaccggcgc gagcagcgtg cccgggcgct tgaacatgtt
     1681 gttcgagtcc gtcgagagct tgcgcatgct gccgcgcatg tagccgttct gcacgaacgt
     1741 ctcgttgccg tcgggacgga cctcgctgac ggtggcctgc aggtccacgt ccggcacggc
     1801 cgagcgcacc cacacgtaca ccgcgccgga gccgagcacg gtggtgtcgt tggcgagcgg
     1861 cgcggtcacg taggacacgg ccgttcccgc cggattgggc ttccagttcc acttccactg
     1921 tgaggcattc ccccacagcc cgccaccgcc ggtgccaccc gcgtagttcg tcagcggcag
     1981 cgcgttcggg tccgatgtgt agcggtcgac gttggttgcg gacggcaggc tgtcacgcag
     2041 cgtgccgttc gcgccgaagt accacgactt ggcgaccgtg cccggcgccg gatacgacga
     2101 gaacgagcgc tcgaacgccg cgtacgggtt gccggcgatc gtgcggccct ccggcgaggc
     2161 gcccgcgccg ttatcgaaca gcacgcgcac catcggcgcc ttttcgaact cggcgagcgc
     2221 gagctcgtag gtcggcatca gctgcaccgg atccaccggc agcagggcgg agtcttgcgg
     2281 cgggatcccc attgctgcat tgaagaccac cggcgccgag gcccgcacga gcgcctgctt
     2341 caggatcggc gcttccttgg ccacgtagag catgagaaag tcgaaccagc gagtgtagat
     2401 ctcgggcgcg agcgagtcga tgtgcggccc gttggtgaag gtgaaccact ttttgtcggt
     2461 gccggtgaag ttgttcacca gctcggggca gtggccgccc gtctgctcgt cctgccactg
     2521 gcacaccagg aacgtgggca cgttgatctt gtgcacgaac gtgttcgggc tcagcggatc
     2581 cgccaccgca gcgtcgtagt gcgtgttcgc ctcgatcgcg gcgagcaggt ccgcggcctc
     2641 gccgtgcagc gcctggttcg cggcgcagat cgtgtcgccc gcctggatgc ggtcccacgc
     2701 ccagcgctgc ccggttgtcg cgctcgcggg ccgcgcgtca cgctggcggg ccagcgccca
     2761 ctcgaccgcg aaaccggtgt tgcggtttcc gcccggatac agggtcgtgg cggtggagtc
     2821 gatgaccgac agcggcgtga tcgcagccag gctcggcggc tgcgtctgcg cggtgaacag
     2881 ctggctgatg ccgccgtagg agatacccat catgccgacc ttgttgtgct tgacccacgg
     2941 ctggcgcgcg acgatctcga cgatgtcgta tccgtcgagg ctttgcatgg tctcgaagaa
     3001 gtcgaacgct ccgccggagc agcccatgcc gcgcatgttg acgttgacca ccgcgaagcc
     3061 catcaggttc gccagcaccg cgatgccgct ctgcgggccg tcgggcttgg cgttggagta
     3121 gcccgagtac tcgatcagcg tcgggtacgg gggcaggtag tcggtgcccg tggcaggaac
     3181 cgggctgccg tgcggcagcc ccgcctggcc ggccgggctc gtcggcggat gcaccgtgta
     3241 ggcgagcttc gtgccgtcgc gcgtggtcag gtacccgtag ccgctcgacg gcatcgactg
     3301 gtggtagatg ctcggatccc acggggccgc ggtcgcgtcg cggacggtca ccgggtcgga
     3361 gtcgccgctg ccgaggcgga cgcggtagcc gtcacccggc gtgaccgtgc ggaacagcac
     3421 gccgccctgg gccgtgacgg tgcgcgtctc gacgacggag ccgtcgggcg cgatcagcgc
     3481 caccgacgcg ccgccgggaa ggccggtcgc gtagacctgc tcgacgctgc cggcgacgtg
     3541 ggcgccggcg gggaaggcgg atgcgctctt gcccgtgagc gagccgagca gcagcgccag
     3601 aacgagcgcc gcccgaacca tcgagcccct cttgatgagt agtccgttgg acatcttcct
     3661 ccgccttccg tcgtctcgcg cagagcgcgc gcatgccccg gccccggccg gtccgtcggc
     3721 ctccacttcg acggggcgcc ctgtttttcc tctttgcgga ccccgcaggg atccgtcccg
     3781 gccgttttgg aggaaggacc cgcttgcctt gaggtatcgc gcggaaatat cgtgcgtatc
     3841 ggccgaaacc cagacacccc gagtgaggat tcgatgcggc gagtgcgcgt gaagctgtcc
     3901 accctgttgg cgttggtcgt cgggctctcc ccgctggccc tcttcgccgc cgacgagatc
     3961 gtcgcctcca gcgaccttgc ccgggtcgtg atcgaattgt ccgaccggcc gctggccacc
     4021 tacgaagacg cgatcccgcg ccttcgcggc gtgctcggcg cccggaccgc cggcgggcat
     4081 ctcgacgtcg ccgcggccgc gagccgcggc tacctgcgct acctggacga gcggcaggac
     4141 gcgttcgagc gccagctcgc cgccgccagc cgcggcgccg agatccactg gcgctaccgc
     4201 atcgccttca acggcatcac cgcgctgctg cggcgcgaca cgatcccggc cgtgcgcgcg
     4261 ctcccaggcg tcgtcgccgt taccgagacc tacgagctcg agcccgagct cgaccagagc
     4321 cgcgacaagc tcgaccttcc ggcgatgtgg tccggcttca aggacagccc gctcggcccg
     4381 ggcgacgggc tgcgcgtcgc gctgctcgac agcggcgtca acccgcatca tccgttcttc
     4441 tcacccgccg gctacaccgc gccgcccggc tatcccaagt ccgagcgcgt ctcgggcggc
     4501 acgcgcaccc cgctgccggc gccgctttac acgagcaaca aggtcatcgt cggcaacgtc
     4561 tacacgccgc cgggcgggac cgcgacaccg tggggtcccg gctcgacgca cggaacacac
     4621 gtcgccggca tcatggccgg ggtcgaaggc acgtacccgt acacgctcgg cggcaagacc
     4681 tgggacctga ccttctcggg cgtgctgccg ggcgcctaca tcatgagcta cctgccggcc
     4741 ggcgactcgg ccgagttctt ggccgcgatc gaggacatcg tggccgacga ggccgacgcg
     4801 ctcaacatca gcctgggcca ctcgcgctgg ctgaccgggg atccggtgca cgacccgctg
     4861 cgaaaggcgc tcgatgccgc ggttgcggcg ggaaccgtcg tggcggcgtc cgcgggcaat
     4921 gcgggatcca acggcgactc gaccgtaacc ggctcctgga agctcagcga caaggtcatc
     4981 accgtcgcca gcaactcgca cgggcgcatg ttcgccaaca agctcagcgt gaccggctcg
     5041 gccacgccgc ccgcgcggct gcgcgacctg atcgccgtgc cggccggcgg aacgacgcca
     5101 ccgttcgcgg tcgaggtctc cggccaatac gtcgtcgcgc ccggcgggaa cggtcccgac
     5161 gccgggctcg cgtgctcgga gctgccgccc gaagaggtgg ccggcaagat cgtgctcgtc
     5221 ggccgaggcg tgtgcacctt cgaggcgaag aaggacaacg tccgcaacgc gggcggcatc
     5281 gcgatgatcg tgcacaacaa cgggcccgac ctcccgacgg ccatgtcgtt caccacgccc
     5341 gcgattcccg ccgcgatgat cagccaggcc gacggccacg cgatgatcac ctgggccaag
     5401 aaggacggcg cgccgaccct ggcggtcgcg cccggcaccg agcgggtcac gaccgccccg
     5461 gccgacgtgg tctcgtcgtt cagcagtcgc ggccccgcgc cgatcatgcg tatcaagccc
     5521 gacatctccg cgcccggctt cagcgtgctg tcctcggtcg tggagggcac caccggcgcg
     5581 gtcggcgctc cgctgttcga gatgctgtcg ggcacctcga tgtccacccc gcacgtgacc
     5641 gccctcgccg cggcgaccaa gggcctgcac ccgacctgga cgccgatgca gatcaagagc
     5701 gcgctgatga acaccgcaca cacctcgatg tatctggacg cgacgcgctc gacgcccgca
     5761 tcggtgcggg atcgcggcgc gggccgcgtg cagccggcgc ggttgctcaa cccggcgctg
     5821 acgttcgacc cgccgagcgt gagcttgggc atggtgcgcg ccggcgagac cgcggcgacc
     5881 acggtcaccg cgaccgacat gcgcggacgc ggtccgtcga agtcgtgggc cgtgtcggtc
     5941 cgcgcggtga agggccacga ccgggtcgtg gtcacgccca cgccgtcacc ggccaccgcg
     6001 cccggcgggg cggcatcgat tgcgattcgc gtctccaccg cgggcgcgcc ggccggcgac
     6061 tacgaaggct tcgtcgagat ccgctccggc agcgagcgct acacgcttcc ctactgggtc
     6121 cgtgtgcagc cggccgcggc caaggacgtg ctgctgatcg actgggaccg caacctgccc
     6181 gaggacctgt cgggcccgta caaggacgcg ctccgcaagc tcggcctgac cttcgacgtg
     6241 ttcgacgggg gaacctcggc cgcggcgaat gccgcccccg gcccgacgtt gctgcaattg
     6301 caggcgtacc ggacggtgat tctgttcacc ggcaacaatt ctgcgagctg gtccaacgcc
     6361 catatcggag ggtccttcgc gctgcagggg tatctgctgg gcggcggccg gttgatcatg
     6421 gcgggacaag acctgcagtc gcagatcgtg aacaatcaga accaaggttc tgattccatg
     6481 ctgacctcga tgggcggctg gattagcgga atgaccgccg tcgagacgcc ggccgcgtgc
     6541 caggtcgtca tcagcgacag caacttctat cgagacgcca ccccgatcga ggacctcacg
     6601 accgtgctcg agctcttcgg cggcaccgtg gacgtcagct caaccgcctc gggcggcccg
     6661 ggcgcgcaga cgcgtcccga cgccggccgc gcggtgaccg ccgacgacga cgtgaatacg
     6721 tgcctgcgcg tctataacgc ggacgcgatc gggccgcacg cgcgcgtgct gggcagctac
     6781 gtcacgacgc gtcgcaacgg catcgacgtc ccgcgcgccg agaacgcggc tgccacgggc
     6841 gtcgcgcccg acaccacgct cgagcgtcgc tcgcgcctgg tgccgtggac ggccgcggtg
     6901 ctgcacgttg gcatcgaagg cgtcaacgag ggccagggcc ggctctcacg tgccgccacc
     6961 ttggggcgtc tctacgactt cgccaacgac accgtgaccg tcgaggtctc gcagacggct
     7021 cgcgacggcg cctccgtcga tctggtcgca aaggcctcct cgacgcgcga cgccaagatc
     7081 gtcaagtacc gctgggactt cggcgacggg tcgggcatca aggaaacgac cacgaaccgg
     7141 gttcgccaga cgtacgcgaa gggcgtctac accgtgcggg tcgaggccgt gaccgagctg
     7201 acccggtccg gggtcggggt agccacgatc gacacgcgca ccagcgtgct caacaaccgc
     7261 acgccgcgaa agcccgctcc cgcgaaggat ctccccgcaa ccgagtacga cggcccgatc
     7321 cccgagacgg acgcgcggcg cggcatcgag cagccgcgtt cggtcctggc ggcgccgaag
     7381 ccggtcctgg aaacgattcg cgggccggtg gatgcgcagc ccgaccacac ctcgtggatc
     7441 ctgctcgcgt tcacgatctg ggccgcgacc gccgcgccgc tgacggcccg gttcgccgga
     7501 gcggggcgcc gccggaccgg gtagccccta tcagcgccga gtgcatccgg tcgtccaaga
     7561 cggtccggtg aaggacgcac ggtggttgta cccaatcctc tccccaattt tttcttgggc
     7621 tggttttgag tagggaattt tggcggaggt gagggtagcg attcttggtt tccgctcggg
     7681 ccgagccatg gaattcatct gcaacgaaag gagcggtctg tgccgacgag ccagatccag
     7741 gtcctgcccg gcggaaatcc tcgggcggcg gcgagctcgt gatccacacc gaagacggca
     7801 cgattcgggc caaggacacg atcggtgccc acgaccgccg agacatccca ggctagcgtt
     7861 catgcacacg gagcggcctc tgcctccgaa agcgaggtcg agccggcatg ggtggcatcg
     7921 ctgcgcgagc acggtcgcgc gggcctgctc tacgcccggc ttcatggggt catggcggct
     7981 ctcgggatca tctttctcgt ggtgttcgta tccgaacgcg tcgcaccgcc cggaagccc
//
LOCUS       DATLFO010000206         3678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_24834, whole genome shotgun sequence.
ACCESSION   DATLFO010000206 DATLFO010000000
VERSION     DATLFO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24834"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VM841_08455"
     CDS             complement(<1..1488)
                     /locus_tag="VM841_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HVL90249.1"
                     /translation="MSSDLSSLPQGSQKRKPRPATGGDTVGGGGPRRPGGFRWKRFGK
                     VAAIVTLIVGVLAAGTGVAVWKYVNDKFAETHDKSIATDPLPPAKPITEPLNILVLGS
                     DRRDVVEGQGELRQFKKDGLEGQRSDTIMLIHIRRGGAGAVGLSFPRDLRVQIPGKKG
                     FHRINAAYAGGPNMVMDTIRELTGLQIHHYVEVNYASFQRIVNAVGGVNLCVTKSYAD
                     AQSGLYISKPGCYDFDGNKALAFVRMRKSDPEGDFGRIKRQQLFIRELMQKVKSVGFL
                     ADVPRVLRLANAVSDGIVTDKNLSLGLVRSIANKLAGYSQSNVDFRTVPSDNKVIGGT
                     AYVVMREEEAKELFAAIAADAELPEFGKTDSSLPGPSDVLLDIRNASNVAGAGRAWAD
                     KLKEAGYRIRLIENADKPRTRTQIAYVPGAELKAKLLAEVFPEAELVRSVNLGNVVDC
                     VVLIGQDVAAEALASPSPALRRPHPDRSAPRSPRRCPGSPTGPARP"
     gene            complement(1507..2526)
                     /locus_tag="VM841_08460"
     CDS             complement(1507..2526)
                     /locus_tag="VM841_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVL90250.1"
                     /translation="MTVRAGLIGFGYWGPMFVRNFGEIDGVETAWVCDRDPDRRAAAA
                     ERYPDVPVYADLDEALAAGADAAVVATPPRTHEPIARAVLEAGLHVLVEKPIAMTGDA
                     ALALGREARQRNLTLMVGHILRYSPALQTIKAMIEGGDLGDLYYLHSTRVNLGRHHRD
                     VNVMWDLGPHDLSAILPLVGGRPEAVSAWGRPFIQQGLEDVVFMYLRFPGGQIAHVHL
                     SWLDPLKVRQIVLVGSKRMLVYDDVNATITLHDKSVDVIPHAETLEEFHLAYRYGESH
                     EVPVAPAEPLAMEITHFLDCIRHGKEPVSGANEGFEVVRVLEAAQQSLVSGGAEVPIE
                     WPTDR"
     gene            complement(2523..>3678)
                     /locus_tag="VM841_08465"
     CDS             complement(2523..>3678)
                     /locus_tag="VM841_08465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVL90251.1"
                     /translation="GEPARFGRYPWLTLMDAPRITIPVIDIAASFATIRAEVDEAIKR
                     VLDAGSFVLGPELAALEDEFAKALNVRRAIGVNSGTDALHLALRAAGVGRGDLVAVPA
                     LTFFATAEAVVHAGATPLIVDVDERTATMSPEALEAVADRVKAVIPVHLYGHPADMDA
                     ILSIAGSRGVAVIEDACQAIGARYKGVAAGAVGDAAAFSFYPTKNIGGAGDGGMVTTK
                     HPHFAATVKSLRHHAQSERKDFHDDIGYNSRLDDLQAAILRVKLRRLDEITKARRQIA
                     AWYHDLLAGLPLELPHEADWAEHVYHLFVVRCDERERVAAGLHERGVGAAIHYPIPLT
                     QQPAINDLGCEQPDAPVAEAIARRQLTIPMYPEMTRPQVEAVAAALKEAL"
BASE COUNT          565 a         1162 c         1328 g          623 t
ORIGIN      
        1 aggccgagct ggtccggtcg gtgaacctgg gcaacgtcgt ggactgcgtg gtgctgatcg
       61 gtcaggatgt ggccgccgaa gcgccggcga ggggctcgcc agcgcttcgg cggccacatc
      121 ctgaccgatc agcaccacgc agtccacgac gttgcccagg ttcaccgacc ggaccagctc
      181 ggcctcgggg aagacctcgg cgagcagctt cgccttgagc tcggcgccgg gcacgtacgc
      241 gatctgggtt cgcgtgcgcg gcttgtcggc gttctcgatc aggcggatcc ggtagccggc
      301 ctctttcagc ttgtcggccc acgcccggcc ggcgccggca acgttgctcg cgttccggat
      361 gtcgagcagc acgtccgagg ggccgggcag cgaagagtcg gtcttgccga actcgggaag
      421 ctcggcgtcg gcggcgatgg cggcgaagag ctccttggcc tcctcttcgc gcatcaccac
      481 gtaggcggtg ccgccgatca ccttgttgtc gctcggcacg gtccggaagt cgacgttgga
      541 ttggctgtag cccgcgagct tgttggcgat ggagcgcacc aggccgagcg agagattctt
      601 gtcggtgacg atgccatcgc tgacggcgtt ggcgagccga agcacacgcg ggacgtcggc
      661 gaggaagccg acgctcttga ccttctgcat cagttcgcgg atgaacagtt gctggcgctt
      721 gatccggccg aagtcgcctt cgggatcgga cttgcgcatt cgcacgaacg cgagcgcctt
      781 gttgccgtcg aagtcgtaac agcccggctt gctgatgtac aggcccgact gcgcgtcggc
      841 gtaggacttg gtcacgcaca ggttcacgcc gccgaccgcg ttgacgatgc gctggaacga
      901 ggcatagttg acctcgacgt agtgatggat ttgaagcccc gtgagctcgc ggatggtatc
      961 catcaccatg ttcgggccac ccgcgtaggc ggcgttgatc cggtgaaagc ccttcttgcc
     1021 ggggatctgg acgcgaaggt cgcgcgggaa cgacaggccg accgcgccgg cgccgccgcg
     1081 gcggatgtgg atgagcatga tcgtgtccga gcgctggccc tccaggccgt ccttcttgaa
     1141 ctgccgaagc tcgccctggc cttcaaccac gtcacgccga tccgagccga gcacgaggat
     1201 gttcagcggc tcggtgatgg gcttggcggg cggaagcgga tcggtcgcga tcgacttgtc
     1261 gtgcgtctcg gcgaacttgt cgttgacgta cttccacacg gccacgccgg tgccggccgc
     1321 gagcacgccg acgatcaggg tcacgatcgc ggcgaccttg ccgaagcgct tccaacggaa
     1381 gccgccgggg cggcgcgggc cgccaccgcc gacggtgtca ccgccggtcg ccgggcgcgg
     1441 ctttctcttc tgcgatcctt gcggaaggga actgagatct gagctcatga ttggtctcgg
     1501 gaaatcttag cggtcggttg gccattcgat ggggacttcg gcgcccccgg atacgagtga
     1561 ctgctgcgcg gcttccagaa cgcgaacgac ctcgaatccc tcgttcgcgc ccgagacggg
     1621 ctctttgccg tgccggatgc agtccaaaaa gtgcgtgatc tccatcgcga gcggctcggc
     1681 cggtgcgacg ggaacctcgt gcgactcgcc gtaccggtag gcgaggtgga actcttcgag
     1741 cgtctcggcg tgggggatca cgtcgacgga cttgtcgtgc agtgtgatgg tcgcgttgac
     1801 gtcgtcgtag accagcattc gtttgctgcc gacgagcacg atctgccgga ccttcagcgg
     1861 atcgagccac gaaagatgaa cgtgggcgat ctggccgccg gggaagcgca ggtacatgaa
     1921 gacgacgtcc tcgaggccct gctggatgaa cgggcggccc caggcgctca cggcttcggg
     1981 gcggccgccg accaggggca ggatggcgga caggtcgtgg ggaccgaggt cccacatcac
     2041 gttgacgtcg cggtgatggc gccccaggtt cacgcgcgtg gagtgaaggt agtacaggtc
     2101 gccgaggtcg ccgccttcga tcatggcctt gatcgtctgc agcgccgggc tgtagcgcag
     2161 gatgtggccg accatcaggg tcaggttgcg ctgccgggct tcccggccga gggcgagcgc
     2221 cgcgtcgccg gtcatcgcga tcggcttctc gacgagcacg tgcaggccgg cttcgagcac
     2281 ggcgcgtgcg atgggctcgt gggtgcgcgg gggggtggcg acgacggcgg cgtccgcgcc
     2341 ggcggcaagg gcttcgtcga ggtccgcgta cacgggcacg tccgggtacc gctcggcggc
     2401 ggcggcgcgg cggtcgggat cgcggtcgca gacccatgcc gtttcgacac cgtcgatctc
     2461 accgaagttc cggacgaaca tcgggcccca gtatccgaag ccgatcaggc cggcgcggac
     2521 ggtcacagcg cttccttcag cgcggctgcg acggcttcga cctgcggccg ggtcatctcg
     2581 ggatacatcg ggatggtgag ctgtcgccgc gcaatcgctt cggcaacggg cgcgtcgggc
     2641 tgctcgcagc cgagatcgtt gatcgcgggc tgctgcgtga gcgggatggg atagtggatc
     2701 gccgcgccga cgccgcgttc gtggagaccg gcggcgacgc gttcgcgctc gtcgcagcgg
     2761 acgacgaaca ggtgatagac gtgctcggcc caatcggcct cgtggggaag ctcgagcggc
     2821 aggccggcga gcaggtcgtg ataccacgcg gcgatctgcc ggcgagcttt ggtgatctcg
     2881 tcgagacgcc gcagcttgac gcgaaggatc gcggcctgaa ggtcgtcgag acggctgttg
     2941 tagccgatgt cgtcgtggaa gtccttgcgc tccgactgcg cgtgatggcg caggctcttg
     3001 accgtcgcgg cgaaatgcgg atgcttcgtg gtgaccatgc cgccgtcgcc ggcaccgccg
     3061 atgttcttcg ttggatagaa cgagaacgcg gcggcgtcgc cgaccgcgcc ggcggcgacg
     3121 cccttgtagc gcgcaccgat cgcttggcat gcatcctcga tcaccgcgac gccgcgggat
     3181 ccggcgatcg acaggatcgc gtccatgtcg gccggatgcc cgtagagatg gaccgggatc
     3241 acggccttga cccggtcggc cacggcttcg agcgcttccg ggctcatcgt cgccgtgcgc
     3301 tcgtccacat cgacgatcag cggcgtcgcg ccggcgtgta cgacggcttc cgcggtcgcg
     3361 aagaacgtga gcgccgggac ggcgaccaga tcgccgcggc cgacgccggc cgcgcgcagc
     3421 gccagatgca gagcgtcggt gccgctgttg acgccgatcg cgcgccgcac attcagcgct
     3481 ttggcgaact catcctcgag cgcggcgagc tcgggaccga ggacgaagga tccggcatcg
     3541 aggactcgtt tgatcgcctc gtccacctcg gcgcggatgg tcgcgaaaga ggcggcgatg
     3601 tcgatgacgg gaatcgtgat cctcggggca tccatgagag tgagccaagg ataccggccg
     3661 aagcgagcag gctcaccc
//
LOCUS       DATLFO010000207        12227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_35134, whole genome shotgun sequence.
ACCESSION   DATLFO010000207 DATLFO010000000
VERSION     DATLFO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35134"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VM841_08470"
     CDS             complement(<1..855)
                     /locus_tag="VM841_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVL90252.1"
                     /translation="MATLYTDARILGPRLELLEADAFVVDRDVFTWAGRLGDAPKVAR
                     CVSLDGRTVTPGFVDGHAHLTMTGLMLGGLDLHGVKSAQALVRRLAGHAEARADVFLY
                     GTGWDDSEWATSPTMTMIDRVAGDRHVYLSRIDAHSALVSRTLLAAARCARMDGAEVG
                     NDGRPTGIVRRDAHHAVRRYFFDRMPMSKIREAHRLAAEAAVAKGITTVHEMSGPLHA
                     AGERDLDQLLKGTLSINVVCYYADEDITVATRRGLKQIGGDLNADGSLGSRTAARRDR
                     DVLVGVVAD"
     gene            complement(855..2009)
                     /locus_tag="VM841_08475"
     CDS             complement(855..2009)
                     /locus_tag="VM841_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90253.1"
                     /translation="MTGHLGLNDEQRALIGLVREVAAREISPYADRWEREGIFPRDAF
                     AALGRAGLLGIPYPEEHGGGAQPSWVYLLCLEELAAALLSVGLGTSVQVLTMFPAAQF
                     GTDEQRKAWLPPACAGEWIGSYCLSESESGSDAAALTTKAVRDGDSYVIDGRKAWVTH
                     GGESDYYVVMARTGEAGPKGISTIFVPASTPGVARAKTEDKMGMKSSPTAQITFDGAR
                     VPAENRIGEEGEGFRIALAALDGGRLGIAACSVGLARAAFDAAVAFAKDRKQFGRPVI
                     ENQGLSWMLADMLTGIEAARTLTLDAAKRRDAGAPFGTHAAMAKLFASDTAMRVTTDA
                     VQIHGGYGYVDEFPVERYMREAKVLQIVEGTNQIQRLVIGRAIAKSSASR"
     gene            complement(2006..3115)
                     /locus_tag="VM841_08480"
     CDS             complement(2006..3115)
                     /locus_tag="VM841_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVL90254.1"
                     /translation="MARIIGRDMSWSRIDRLRAALREAGVAALMAGPSADLRYLTGYD
                     ALPLERMTLLVVRPDDEPFLVVPGLERLRAEEFTAGIELVPFGETDDPVALVASRLGA
                     LDAPVAVADRMWAMFVLRLQAAFDRAVFVPASTIMRTLRIRKDDAELDALARSAAAAD
                     RVAALIAGASVAGKREREGSRWISDALIAEGLEQVNFAIVAAGPNAASPHHEPGERII
                     EAGDALVCDFGGTLDGYCSDITRTFFVGDPPSEFVALYDVLQEAQEAAVNAVKPGVTA
                     ESVDAAAREIIAAAGYGEHFIHRTGHGIGLEEHEDPYIVAGNTEPLEPGMCFSVEPGI
                     YIPGRFGARIEDIVVCTDAGARRLNNASRDLKVIA"
     gene            3114..4757
                     /locus_tag="VM841_08485"
     CDS             3114..4757
                     /locus_tag="VM841_08485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HVL90255.1"
                     /translation="MPPIGPDTRAEMLDRLASEQFDVLIVGGGVTGAGCALDAASRGL
                     KTALVEKRDFASGTSSKSSKLVHGGLRYLAQYEFGLTWEAAHERDLLARLCPHLVRPL
                     QFLYPVFRKGKEARFAAYGLTIYDALSARRGVPRHTRANARRIESLAPSIDTGRVVSA
                     WTYWDASTDDARLVFELIRSATRFGAATANYAAVDGFVHDAGGRLAAARVTDTRTGRG
                     FEVRARVIVNATGIWAGEVGVMEDADTPIALRPAKGIHLMIPRERIALGAACIVPSGA
                     RDRRSLFLQPWADRIMLGTTDTEYDGPLDAPSVTEADVDYVLHGVNKWMNAGLTREDL
                     VGAWAGLRPLRAAATTERTADLSRRHAIQVSPGGLISVTGGKLTTFRRMARDAVDAAC
                     ETLGVKRRSVTHRIRIGLTKPAVEVRGDVADLLAAAGADPDQARRLVEAFGDLAVHVA
                     ATAAEDPSLASPVAAGLPVIGAEVRWAATREMAVTLEDVLARRTRLSLADRDGGLAAT
                     GLVAAARGWGEDETAAERARYVAGLAAERGPGIAAPAMA"
     gene            4959..7043
                     /locus_tag="VM841_08490"
     CDS             4959..7043
                     /locus_tag="VM841_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90256.1"
                     /translation="MWRRLAAGVLATLALSAVLGQSPPATTRAVGPQTCGPTTLHGTI
                     VYKDGKLVCGPGQPLKLREDLGTKAKPGRRGTPILSFAALTDFQLPDEESPLRGEFFD
                     KCTTHELDAAFRWNDTLLPHLLNSHIQAVNRMQRGPVTGRPFDFAVQLGDAAENQQYN
                     EVRAFIDLLDGGTVVDPDSGRDGYDGNQGSDPYASPVKGRSLRDLANEPFWATGLRRP
                     DGSPLPWYTVMGNHDAKVRGVMPNSEAWKTFAKAWVTGGLMVNDLPPDMQQRVCKDPA
                     VMLDPAFWATLAAHPGSVRLVEADPERRLLDREDWLREHDTSRGLPAGHRGTMCPVKD
                     VAGRQNRACYTVDVPPAIPGEPPIHLIVLDTAADEGLDSGNIDQAQWDWLNKDLKKHS
                     SCFYATDNALTCDETGNPTSLIVVMSHHSSAKMSNTTPRTDGGTAKTGADLQKLLLLF
                     PNVIMHAAGHTHQNKIWQHMRANGTGGYFEVNTSAIADWPHQSRTIEIVDNHDGTMSI
                     YAITFDGAAPVDPATMRWSDDPTPETDARFGKQKAAINEEYLAAVARWVGANDPQHGH
                     DAIENLATDMDKNVELILTHPTGGAKRPGLLDFKPPVYPRPRFPFPWPFPQPQFPIAA
                     PTGPFPFPSTPVTPPQFPSAGIPAQPTSRGIGSPADGPALQLREYVMTVLAAAGVMWL
                     MRSRIRRNQIGL"
     gene            7065..7934
                     /locus_tag="VM841_08495"
     CDS             7065..7934
                     /locus_tag="VM841_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90257.1"
                     /translation="MSGLPRSVERWRDAGRFIDAGPHRVFMTGDGERGPDVLILHGFP
                     ASSYQWRNVIPHLAGKARVTAFDFLGYGLSDKPADPRVTLFDQAGLAEHVAAGRGIER
                     CAIVAHDMGDTVAAELLHRQNAGELPFEIDRVILTNGSIYIDMAQLSAGQQLLLQLPD
                     EPLAESLPLDGFKPGITETFSREHPPGDAEVEALLALIAHNDGDRLLPRLIRYIEERR
                     QNQQRWTDALNEFTGLLWAVWGEQDPIAVVAMAHELKAQRPMTEIEIWPDVGHWPEIE
                     VPERLAGAVMERL"
     gene            8099..9010
                     /locus_tag="VM841_08500"
     CDS             8099..9010
                     /locus_tag="VM841_08500"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HVL90258.1"
                     /translation="MSSSHARMHLPVALRRTVALALAAAGILGSLAVPAEAAYPQGQF
                     IIQCLGPVRSAPDDPIVSPGAADSSHLHEFYGNTAIHPSSTYESLIVAATGCEHSKAG
                     DEPGDTASYWHPTVSVNGARVAAPKSTFYYSNRARKKPGTMKAWPAGLKAIAGNGKAT
                     SPQSTSIVYWGCGDGSSVSKVNMPPQCKAGDAGLTVHVIYPDCWDGVRLDSPDHKSHL
                     AYSNKSSVCPSTHPVSLPWLMQRFQWTNFYPAPSSITLSSGPAYTFHSDFWNTWNQAR
                     LEQLVAFCINAGKKCVYADLDNLKPPA"
     gene            complement(9011..9979)
                     /locus_tag="VM841_08505"
     CDS             complement(9011..9979)
                     /locus_tag="VM841_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVL90259.1"
                     /translation="MKSPFGPMRVIINPRAGRGAVERGWPEAKAVLDAAGIDYRVTVT
                     EEAGHATRAAREALTGGEKFVVAVGGDGTVNEVINGLMGSGGVADPDVVLGVVAAGSG
                     CDFVKTFGLSQDPAVAAKNLLGGNLWGEIDVSRVRFTAGDGSQQTRWFGNIAEAGLGA
                     DVVAAAAKMPKWLGGKVYKLAAVKEIARHEPFQARVSMHGRKARGLPVDAPLSEVAWD
                     GEMSLLVVANCQFFGGGMRVAPRAIPGDGMLDVQIATGTKSDAMKVMQKIAKGRHIPN
                     KGILEYLATSVTLDGPKPILIEADGEVLGTTPATFDVIPAALKMKV"
     gene            complement(9976..11370)
                     /locus_tag="VM841_08510"
     CDS             complement(9976..11370)
                     /locus_tag="VM841_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL90260.1"
                     /translation="MSLDELASLLPGGTISTDDADLAAHARDWWPGALLRERSGEAPV
                     RPAAVVRPSSAQEVASVLAWADRTRTPVVPFGGGSGVCGAAQAVEGALALDTRRLDSI
                     EIDDETLMVTAGAGVMGHALEDALSRRGLTAGHFPQSIDISTVGGWCATRGAGQKSGG
                     YGRIDDMVLGLEVALPGGRLVRFASHPGTSAGPDLKRMFLGSEGTLGVITEVTLAAYP
                     QPASVAHETFAFVSFGDGLRALRRLARDGVLPAVARLYDETDAGIAFREDAPGGSVLI
                     VRHEDSGAGGASRRESVNAIAGDAGGRALGPGLSEAWWTRRNHAVETFGKIMHEGLLG
                     PTGAVDTMEIAGAWPADALYAAVCAALSSHADVVACHASHVYPQGVCLYFTFVLLGSA
                     DDTITRRRYDAAWSAGMEAAIAGGATITHHHGVGLLRAPWIERELGEAMSVWRAVKAA
                     LDPNGIMNPGKAGL"
     gene            complement(11422..>12227)
                     /locus_tag="VM841_08515"
     CDS             complement(11422..>12227)
                     /locus_tag="VM841_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90261.1"
                     /translation="FVAAPTSVTHAPVAVANYRTSIPIRIDANCGAPCTATLFFRRTG
                     LLLPTFLSGGDPGPNPADAADVQWASVSMKLDAVNNAFQGQGVGLYTFSAQIPSAYVD
                     TRGVDYAIRVTDGKTKTYWPGSVYNGYAAPSDGTRYGWQHVHVLNPAMAWHTQTQYST
                     ARNTSLTLQMQALCWTEACSAQIRYHSFGGPIVTANMTQTAVLFFGQARMITYSYTVP
                     ASSMTTLTLSYAFKFTDGFTTSYSPGTVYNGYYEKRDGVQAGEYQVVLL"
BASE COUNT         1892 a         4339 c         4177 g         1819 t
ORIGIN      
        1 gtctgctact acgccgacga ggacatcacg gtcgcgacgc gccgcggtgc gggagcccag
       61 cgagccgtcg gcgttcagat ccccgccgat ctgcttgagg ccgcggcgcg tcgcgaccgt
      121 gatgtcctcg tcggcgtagt agcagaccac gttgatcgaa agcgtgcctt tcagaagctg
      181 atccaggtcg cgctccccgg ccgcgtgcag cgggccgctc atctcgtgca cggtcgtgat
      241 gcctttcgcg acggcggcct cggcggcgag gcggtgcgcc tcgcggatct tcgacatcgg
      301 catgcggtcg aagaaatagc gccgcaccgc atggtgcgcg tcgcgccgca cgatgccggt
      361 cggccggccg tcgttgccga cctcggcccc gtccatccgc gcacaccgcg cggccgccag
      421 cagcgtgcgc gagaccaagg cggaatgggc gtcgatgcgc gacaagtaga cgtgccggtc
      481 cccggcgacg cggtcgatca tcgtcatcgt cggggacgtc gcccactcgc tgtcgtccca
      541 gccggtcccg taaagaaaca cgtcggcacg ggcctccgcg tgaccggcga gtcggcggac
      601 cagcgcctgc gcgctcttga ccccgtgcag gtcgagcccg ccgagcatca ggccggtcat
      661 cgtcaggtgc gcgtgaccgt ccacgaaacc gggcgtgacc gtccggccgt ccagcgagac
      721 gcaccgcgcc actttcgggg catcgccgag acgtccggcc caggtgaaca catcgcgatc
      781 caccacgaac gcatcggcct cgagcagctc cagtcgcggc cccaggatcc gcgcgtccgt
      841 gtagagcgtt gccatcagcg ggacgccgag ctcttggcga tggcgcggcc gatgacgaga
      901 cgttggatct ggttggtccc ttcgacgatc tgcagcacct tggcttcgcg catgtagcgc
      961 tcgacgggga actcgtccac gtagccgtag ccgccgtgga tctgtaccgc gtcggtggtg
     1021 acgcgcatcg cggtgtcgga ggcgaacagc tttgccatcg cggcgtgggt gccgaacggc
     1081 gcgcctgcgt cgcggcgctt ggcggcgtcg agcgtgagcg tgcgcgcggc ctcgatgccg
     1141 gtcagcatgt cggcgagcat ccacgacaag ccttggttct cgatgaccgg ccggccgaac
     1201 tgcttgcgat ccttggcgaa ggccacggcg gcgtcgaagg cggcgcgggc cagcccgacg
     1261 ctgcacgcgg cgatgccgag ccgtccgccg tcgagcgcgg cgagcgcgat ccggaagccc
     1321 tcgccctcct cgccgatgcg gttctcggcg gggacgcgcg cgccgtcgaa ggtgatttgc
     1381 gcggtcggcg aggacttcat ccccatcttg tcctcggtct tagcgcgcgc gacgcccggc
     1441 gtggatgcgg gcacgaagat ggtcgagatg cctttcgggc cggcctcacc ggtccgggcc
     1501 atgaccacgt agtagtcgga ctcgccgccg tgcgtcaccc acgccttacg gccgtcgatg
     1561 acgtaggagt cgccgtcgcg aaccgccttg gtggtcagcg ccgcggcgtc cgatccgctt
     1621 tcggactcgc tcaggcagta cgagccgatc cactcgcccg cgcacgcggg cggcagccac
     1681 gccttgcgct gctcgtcggt tccgaactgc gcggccggga acatcgtgag cacctgcaca
     1741 ctggttccca ggccgaccga cagcaacgcg gccgcgagct cttcgaggca cagcaggtac
     1801 acccagctcg gctgcgcgcc gccgccgtgc tcctccgggt acgggatgcc gagcagcccc
     1861 gcgcggccga gcgcggcgaa cgcgtcgcgc ggaaagattc cttcgcgctc ccagcggtct
     1921 gcgtacggcg agatttcacg cgccgcgacc tcgcgcacca gcccgatcag tgcgcgctgc
     1981 tcgtcgttca agccgagatg gccggtcatg cgatcacttt caggtcgcgc gaggcgttgt
     2041 tgaggcggcg agcgcccgcg tcggtgcaca caacgatgtc ttcgatgcgc gcgccgaagc
     2101 ggccggggat gtagatgccg ggctcgacgc tgaagcacat gccgggctcc agcggctcgg
     2161 tgttgccggc gacgatgtag ggatcttcgt gctcttccag gccgatgccg tgcccggttc
     2221 ggtggatgaa gtgctcgccg tagccggcgg cggcgatgat ctcgcgcgcg gccgcgtcga
     2281 ccgactcggc agtgacgccg ggcttgaccg cgttgacggc ggcctcctgc gcttcctgca
     2341 gcacgtcgta cagagcgacg aactcggacg gcggatcgcc gacgaaaaag gtgcgcgtga
     2401 tgtcgctgca gtagccgtcg agcgtaccgc cgaagtcgca cacgagcgca tcacccgctt
     2461 cgatgatgcg ctcgccgggc tcgtggtgcg gggaggcagc gttgggtccg gcggcgacga
     2521 tcgcgaagtt gacctgctcg agcccttcgg cgatgagcgc gtcgctgatc cagcgcgacc
     2581 cctcgcgctc ccgcttgccc gcgacggacg cgccggcgat gagcgcggca acgcggtccg
     2641 cggcggcggc actgcgcgcg agcgcgtcga gctcggcgtc atcctttcgg atccgcagcg
     2701 tgcgcatgat cgtcgaggcc ggaacgaaca cggcccgatc gaaggcggcc tgcaggcgca
     2761 gcacgaacat cgcccacatg cgatccgcga ccgcgacagg cgcgtcgagg gcgcccagcc
     2821 gcgatgcgac gagcgcgacc ggatcgtcgg tctcgccgaa ggggaccagc tcgatcccgg
     2881 cggtaaactc ctcggcgcgc agccgctcga gccccggcac gaccaggaac ggttcgtcgt
     2941 cggggcgcac gaccagcagc gtcatgcgct cgagcggcag cgcgtcgtag ccggtcaggt
     3001 atcgcaggtc ggcggacggg ccggccatga gcgcggcgac gccggcctcg cgcagggcgg
     3061 cgcgcaagcg atcgatccgg gaccaggaca tgtcgcggcc tatgatacgg gccatgccgc
     3121 cgatcggacc cgacacccgc gccgagatgc tcgaccggct cgcctcggaa cagttcgacg
     3181 tgctgatcgt cggcggcggc gtcaccggcg ccggctgcgc gctcgacgcc gcctcgcgcg
     3241 gactcaagac cgcgctggtc gagaagcgag acttcgcgtc gggcacatcg agcaaatcct
     3301 cgaagctcgt gcacggcggg ctccgctacc tcgcccaata cgagttcggc ctgacctggg
     3361 aagccgcaca cgagcgcgat ctgctcgcac gcctgtgccc gcacctggtg cggccgctgc
     3421 agttcctgta tccggtgttt cgcaagggca aggaggcgcg cttcgcggcc tacgggctca
     3481 cgatctacga cgcgctcagc gcccggcgcg gcgtgccgcg gcacacgcgc gccaacgcgc
     3541 gccgcatcga gtccctcgcc ccctcgatcg acaccggccg ggtcgtatcc gcatggacct
     3601 actgggacgc gtcgaccgac gacgcgcggc tggtgttcga gctgatccgg tccgcgacgc
     3661 gcttcggggc cgcgaccgcg aactacgcgg ccgtggacgg cttcgtgcac gacgcgggcg
     3721 ggcggctggc cgcggcccgc gtcaccgaca cgcgcaccgg ccgcggcttc gaagtccggg
     3781 cgagggtgat cgtgaacgcg accggcatct gggcgggcga ggtcggcgtg atggaggatg
     3841 cggacacgcc gatcgccctg cggccggcga agggcatcca tctgatgatc ccgcgcgagc
     3901 ggatcgcgct cggtgcggcg tgcatcgtgc cgtcgggcgc ccgcgaccgg cgctcgctgt
     3961 tcctgcagcc gtgggcggac cggatcatgc tcggcacgac cgacaccgaa tacgacggcc
     4021 cgctcgacgc gccgtcggtc accgaagccg acgtggacta cgtgctccac ggcgtcaaca
     4081 aatggatgaa cgccggcctg acgcgtgagg acctggtcgg cgcgtgggcc ggcctgcggc
     4141 cgctacgcgc cgcggcgacc acggaacgga ccgcggacct gtcgcggagg cacgcaatcc
     4201 aggtctcgcc cggcggcttg atctcggtga cgggaggaaa gctgacgacg ttccgccgca
     4261 tggcccgcga cgccgtggat gccgcctgcg agacgctcgg cgtgaaacgg cgcagcgtca
     4321 cgcatcggat caggatcgga ttgaccaagc cggccgtcga ggtacggggc gacgtggccg
     4381 acctgctcgc cgcggccggg gccgacccgg atcaggcccg gcgtctggtc gaggcgttcg
     4441 gcgacctggc cgtgcacgtc gcggcgacgg cggccgagga tccctcgctg gcttcgcccg
     4501 tcgcggccgg gctgccggtc atcggcgcgg aggtgcgctg ggccgcgacc cgcgagatgg
     4561 ccgtcacgct cgaggacgtt ctggcgcggc gaacacggct gtcgctggcc gaccgggacg
     4621 gcggcctggc ggcgaccggc ctggtcgccg cggcgagggg ctggggcgag gacgagaccg
     4681 cggcggaacg cgcgcgctat gtcgcggggc tcgccgccga gcgcggcccc ggcatcgcgg
     4741 ctcccgcgat ggcctgatcg gccgactctg ccgcacgatc ccgcttcgct cgcatccggc
     4801 cgaaaggacc cggttccgat tgcgccgaat tcggtcagca gggacgctca gccgactctc
     4861 atggaggctg gtagcctcga atcgggacat cgcggaccat aaccggctcg gtgggacggt
     4921 cgttgactgg tcggaggtat acgacactgg acgctccatt gtggcgacgc ctggcggcgg
     4981 gagtcctggc gactctcgcg ctgtcggccg tgctcggtca gtccccgccc gccaccacgc
     5041 gcgcggtggg cccgcagaca tgcggcccga cgaccctgca cggcacgatc gtctacaagg
     5101 acggcaagct cgtgtgcggc cccggccagc cgctgaagct gcgagaggat ctgggcacca
     5161 aggccaagcc cggccgtcgt ggcacgccga tcctctcgtt cgcggcgctc accgacttcc
     5221 agcttcccga cgaggagtcg cccttgcgcg gcgagttctt cgacaagtgc accacgcacg
     5281 agctcgacgc ggcgttccga tggaacgaca cgctcctgcc tcaccttttg aactcgcaca
     5341 tccaggccgt caaccggatg cagcggggcc cggtcaccgg ccgtccgttc gacttcgcgg
     5401 tgcagctcgg ggacgcggcc gagaaccagc agtacaacga ggtgcgtgcg ttcatagacc
     5461 tgctcgacgg cggaaccgtg gtcgatccgg actcgggccg tgacggctac gacggcaacc
     5521 aaggatccga tccgtacgcc agcccggtga aggggcgctc gctgcgcgat ctggccaacg
     5581 agccgttctg ggcgaccgga cttcgccggc ccgacggctc gccgctcccc tggtacacgg
     5641 tgatgggcaa ccacgacgcg aaggtgcgcg gcgtgatgcc aaacagcgag gcgtggaaga
     5701 cgttcgccaa ggcgtgggtg accggcggtc tgatggtcaa cgatctcccg ccggacatgc
     5761 agcagcgcgt ctgcaaagac cctgcggtca tgctcgaccc ggccttctgg gcaacgctgg
     5821 ccgcccatcc cggaagcgtg cgtctggtcg aggccgaccc ggagcggcgg ctgctcgacc
     5881 gcgaggactg gctgcgcgag cacgatacga gccgcggcct gcccgccggc catcgcggca
     5941 cgatgtgccc ggtcaaggac gtggcgggcc ggcagaaccg cgcctgctac accgtggacg
     6001 tgccgccggc gatcccgggt gagccgccga ttcatctgat cgtgctcgac accgccgccg
     6061 acgagggcct ggactcgggc aacatcgacc aggcccaatg ggactggctg aacaaggatc
     6121 tcaagaagca ttcgagctgt ttctacgcga ccgacaacgc gctcacgtgc gacgagaccg
     6181 gcaacccgac gtcgctgatc gtagtgatga gccatcactc gtcggcgaag atgagcaaca
     6241 ccacgccacg caccgacgga ggcaccgcca agacgggcgc cgaccttcag aagctgctgc
     6301 tgctgttccc gaacgtgatc atgcacgcgg ccggccatac gcatcagaac aagatctggc
     6361 agcacatgcg cgccaacggc accggcggct acttcgaggt gaacacgagc gcgatcgcgg
     6421 actggcctca tcagtcccgc acgatcgaga tcgtggacaa ccacgacggc acgatgtcga
     6481 tctacgcgat caccttcgac ggcgccgcgc ccgtggaccc ggcgaccatg cgctggagcg
     6541 acgatcccac gcccgagacc gacgcgcggt tcggcaagca gaaggccgcg atcaacgagg
     6601 agtatctcgc tgccgtggcc cggtgggtgg gcgcgaacga tccgcagcac ggccacgacg
     6661 cgatcgagaa cctggccacc gacatggaca agaacgtcga gctgatcctg acgcatccga
     6721 ccggcggcgc gaaacgcccc ggcctgctcg acttcaaacc gcccgtctat ccgcggccgc
     6781 gcttcccgtt cccctggccg ttcccgcagc cccagttccc gatcgcggcg ccgaccggac
     6841 cgttcccgtt cccgagcacg ccggtgacgc cgccgcagtt cccgtccgcg ggaattccgg
     6901 cccagcccac ctcgcgcggg atcggctcgc ccgccgacgg accggcgttg cagcttcgcg
     6961 agtacgtgat gaccgtgctg gcggccgctg gcgtcatgtg gctgatgcgc agccgcatcc
     7021 ggcgcaacca gatcggcctg taggctcgcg tatgctggcc cgcaatgagc gggctcccac
     7081 gttccgtcga acgctggcgt gatgccggac gcttcatcga cgccggtccg caccgcgtgt
     7141 tcatgaccgg cgacggagag cgcggccccg acgtgctgat cctgcacggc ttccccgcct
     7201 cgtcctacca atggcgcaac gtcatcccgc atctggccgg caaagcgcgc gtcaccgcgt
     7261 tcgacttcct cggttacggc ctgagcgaca agcccgccga tccgcgtgtc acgctcttcg
     7321 atcaagccgg cctggccgag cacgtcgctg ccggccgcgg catcgagcgc tgcgcgatcg
     7381 tcgcgcacga catgggcgac accgtcgcgg ccgagcttct tcaccgtcag aacgcaggcg
     7441 agctgccgtt cgagatcgac cgcgtgatcc tgaccaacgg ctcgatctac atcgacatgg
     7501 cgcagctgtc ggccggccag cagcttctgc tgcagcttcc cgacgagccg ctggccgagt
     7561 cgctgccgct cgacggcttc aaacccggca tcaccgagac gttttcgagg gagcatccgc
     7621 cgggcgacgc cgaagtcgaa gcgctgctcg cgctgatcgc ccacaacgac ggcgaccgtc
     7681 tgctgccgcg cctgatccgc tacatcgaag agcgccgcca aaaccagcaa cgctggaccg
     7741 acgcgctcaa cgaattcacc ggcctgctct gggccgtctg gggtgagcag gatcccatcg
     7801 cggtggtcgc gatggcccac gagctgaagg cccagcgtcc catgaccgag atcgagatct
     7861 ggccggacgt tggccactgg cccgagatcg aggtgccgga gcggctggcc ggcgcggtca
     7921 tggaacgcct ctagccggtc ccggagggcc cgcacccacg cggaacgcac atcgttgcgt
     7981 ccgcagagcg cggacgcact gctgagtcat tcgactctgt cgccggcacg cggacgaccc
     8041 gatatcgctg aagaagctcg ctcgagcttc cgctcgctca cctcaaggag accggccgat
     8101 gtcgtcctcg cacgcccgca tgcacctgcc cgttgccctc cggcgcacgg tcgcgctcgc
     8161 gctcgccgcg gcgggcatcc tcggcagcct tgccgttccg gccgaagccg cctatccgca
     8221 gggacagttc atcatccaat gcctcggacc ggtccggtcg gcgcccgacg acccgatcgt
     8281 gtctcccggt gcggccgact cgtcgcatct tcacgagttc tacggcaaca cggccatcca
     8341 cccgtcgagc acgtacgagt cactgatcgt cgcggcgacc ggatgcgagc attcgaaggc
     8401 gggagacgag ccgggcgaca cggcctcgta ttggcatccg acggtctccg tgaacggcgc
     8461 gcgggtcgcc gctccgaagt cgacgttcta ctactcgaat cgtgcgcgca aaaagccggg
     8521 cacgatgaag gcgtggccgg cggggctgaa ggcgatcgcg ggcaacggca aggccacgag
     8581 cccgcagagc acgagcatcg tctactgggg atgcggcgac ggctcgtcgg tgtccaaggt
     8641 caacatgccg ccgcagtgca aggccggcga cgcgggcctg acggttcacg tgatctaccc
     8701 ggactgttgg gacggcgtgc gtttggactc gcccgaccac aagagccatc ttgcctactc
     8761 gaacaaaagc tcggtgtgtc cgtccacgca tccggtgtcg ctgccgtggc tgatgcagcg
     8821 cttccaatgg acgaacttct atccggcgcc ctcgtcgatc accctgtcga gcggtccggc
     8881 gtacaccttc cactcggact tctggaacac ctggaatcag gcgcgtctgg agcaactggt
     8941 cgcgttctgc atcaatgccg gcaagaagtg cgtctatgcg gatctggaca acttgaagcc
     9001 gccggcgtag ctagaccttc atcttcaatg cggcggggat gacgtcgaac gtggcggggg
     9061 tggtgcccag cacctccccg tccgcttcga tcaggatggg tttgggacca tccagcgtca
     9121 ccgaagtggc gagatactcg agaattccct tgttcgggat gtgccggcct ttggcgatct
     9181 tctgcatgac cttcatcgcg tcggacttcg tgccggtcgc gatctgcacg tcgagcatgc
     9241 cgtcgccggg gatcgcgcgc ggcgcgacgc gcatgccccc accgaagaac tgacagttcg
     9301 ccacgaccag cagcgacatc tcgccgtccc acgcgacttc gctcagcggc gcgtccacgg
     9361 gcaagccgcg cgctttgcgg ccgtgcatgg agacgcgggc ttggaacggc tcgtggcggg
     9421 cgatctcttt gacggcggcg agcttgtaga cctttccgcc gagccacttg ggcatcttcg
     9481 ccgcggcggc gaccacgtcg gcgcccaagc cggcttcggc gatgttgccg aaccatcggg
     9541 tctgctgcga accgtcccca gccgtgaagc gaacgcgcga gacgtcgatc tcgccccaca
     9601 ggttgccgcc cagcagattc tttgcggcga cggcgggatc ttgagacagc ccgaaggtct
     9661 tgacgaagtc gcagccgctt ccggcggcga ccacgccgag caccacgtcg gggtcggcga
     9721 cgccgccgga tcccatcagg ccgttgatga cctcgttgac ggtcccgtcg ccgccgaccg
     9781 cgacgacgaa cttctctccg ccggtgagcg cttcgcgcgc ggcccgggtg gcgtggccgg
     9841 cttcttcggt gacggtgacg cgatagtcga tgcctgctgc gtcgagcacg gctttcgcct
     9901 cgggccagcc gcgctcgacc gctccacggc ccgcgcgcgg gttgatgatc acccgcatcg
     9961 gtccgaaggg actcttcata ggcccgcctt tccgggattc atgatcccgt tcgggtcgag
    10021 cgctgccttc accgctcgcc acacgctcat cgcctccccc agctcgcgtt cgatccacgg
    10081 cgcgcgcagc aggccgacgc cgtgatggtg cgtgatcgtg gcaccgcccg cgatcgccgc
    10141 ctccatgccc gcgctccacg ccgcgtcgta gcgccgccgc gtgatcgtat cgtcggccga
    10201 gcccagcagc acgaaggtga agtacaggca cacgccctgc ggatacacgt gcgaggcatg
    10261 gcatgcgacg acgtcggcat gggacgacag cgccgcgcac acggccgcgt agagcgcatc
    10321 ggccggccag gctccggcga tctccatcgt gtccaccgcg ccggtcggcc cgagcaggcc
    10381 ttcgtgcatg atctttccga aggtctcgac cgcgtggttg cggcgcgtcc accatgcttc
    10441 cgagagcccc ggccccaggg cgcggccgcc cgcgtcgccc gcgatcgcat tcacgctctc
    10501 gcggcgcgac gcgccgcccg cgccgctgtc ttcgtggcga acgatcagca cgctgccgcc
    10561 gggcgcgtcc tcgcggaagg cgattcccgc gtcggtttcg tcgtagaggc gggcgaccgc
    10621 gggcagcacg ccgtcacgcg cgaggcgccg caatgcgcgc aggccgtccc cgaacgagac
    10681 gaacgcgaag gtctcgtgcg caacggaggc cggctgcgga tacgcagcga gcgtcacctc
    10741 ggtgatcacg ccgagcgtcc cttcgctgcc gaggaacatg cgcttgaggt cgggacctgc
    10801 gctggtgccg ggatgggagg cgaagcgcac gagccggccc ccgggaagcg caacctcaag
    10861 accaagcacc atgtcgtcga tccggccgta gccgccggac ttctgacccg cgccgcgtgt
    10921 cgcgcaccag ccgccgacgg tcgagatgtc gatcgattgc ggaaagtggc ccgcggtcag
    10981 accgcggcgg ctgagcgcat cctccagcgc gtgacccatc acaccggcac ccgcggtcac
    11041 catgagcgtc tcgtcgtcga tctcgatcga gtccagccgc cgcgtgtcga gcgcgagcgc
    11101 gccctccacg gcctgcgcgg cgccgcacac ccccgagccg ccgccgaagg gcaccaccgg
    11161 cgtgcgggtc cggtcggccc aggccagcac cgacgcaacc tcctgcgcgg acgacggacg
    11221 caccaccgcc gccggccgca ccggcgcctc gccggatcgc tcgcgcagca gggcgccggg
    11281 ccaccagtcg cgcgcgtgcg cggccagatc ggcatcgtcg gtggagatcg tgccgccggg
    11341 caggagtgag gcgagctcgt cgaggctcat cgcgcctcgg atttcaccgc gccgggcgcg
    11401 gcaacgcaac agcggctccg gctacagaag cacgacctgg tactcgccgg cctggacgcc
    11461 gtcccgtttc tcgtagtagc cgttgtagac ggtgccgggc gagtacgagg tggtaaagcc
    11521 atcggtgaac ttgaacgcgt acgaaagcgt cagggtggtc atcgacgagg ccgggaccgt
    11581 gtacgagtac gtgatcatcc tcgcctgccc gaagaacagc acggccgttt gcgtcatgtt
    11641 cgccgtcacg atcggtccgc cgaagctgtg atagcggatc tgcgccgagc atgcctcggt
    11701 ccagcaaagt gcctgcatct gcagggtcag ggacgtgttg cgcgccgtcg agtactgcgt
    11761 ctgcgtgtgc caggccatcg cggggttgag cacgtgcacg tgctgccagc cgtagcgcgt
    11821 gccgtccgac ggcgcggcat agccgttgta gacgcttccc ggccagtagg tcttggtctt
    11881 gccgtcggtc acccggatcg cgtagtccac cccgcgcgtg tccacgtacg cggacgggat
    11941 ctgcgccgag aacgtgtaca gcccgacgcc ctggccctgg aacgcgttgt tgaccgcatc
    12001 gagcttcatc gacaccgatg cccactgaac gtcggcggcg tcagccgggt tcgggcccgg
    12061 atcgcctccg ctcaggaacg tcggcagcag cagccccgtt cgccggaaga acagcgtcgc
    12121 cgtgcacggt gcgccgcagt tcgcgtcgat gcgaatcgga atcgaggttc ggtagttcgc
    12181 caccgcgacg ggtgcgtgcg tcaccgacgt cggcgcggcg acgaaga
//
LOCUS       DATLFO010000208         2341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_74121, whole genome shotgun sequence.
ACCESSION   DATLFO010000208 DATLFO010000000
VERSION     DATLFO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74121"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VM841_08520"
     CDS             complement(<1..278)
                     /locus_tag="VM841_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HVL90262.1"
                     /translation="MRMRTGALMYRAVRKSLKVVARVVVGAVVFGILVARTDLGELGR
                     QLRQAEVVPIGFAALLFLVGLGVSALRWREYLGALEYPMPYATLYRLY"
     gene            265..1380
                     /locus_tag="VM841_08525"
     CDS             265..1380
                     /locus_tag="VM841_08525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL90263.1"
                     /translation="MRILIVNYEYPPLGGGGGVLARALVRELGRDHDVTVLTSRGPGL
                     RPESWEDGARIVRVSVVRRTERERASMASLVSFGPAARRTGAKLIQQRRFDLVHTFFA
                     VPSGPAGVALARRGGVPHLLTVCGADIYDPTRPLSPERFAPVRATVKRVVGASERVIA
                     ISKDLADRTKKLTGRQDVDIVGIGVEPAVLPPRSRPADGVTRFVTVARLVQRKALGAV
                     VRSVPDGCHLVVVGEGPERATLERAATPGRVTFAGALDDAAKAQALADADAFVLASLH
                     EAFGIVYLEAMQAGLPVIAGSIGGQTDFLTHDENALLVPAGDPVRLGEAMKRMAADPA
                     KRARMGLAAKETAQRYTPQWMAKEYLDRYERALATEA"
     gene            complement(1367..>2341)
                     /locus_tag="VM841_08530"
     CDS             complement(1367..>2341)
                     /locus_tag="VM841_08530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL90264.1"
                     /translation="VYNERDSLAPLLSELVAARRAIPPSEVVFVDDGSSDGSTQVLED
                     AHQKDPTIRVVRFTRNFGQTAALAAGFDYAHGRVIVTMDADLQNDPADIPKMLAKLDE
                     GYDVVSGWRVDRKDTLWTRRLPSKVANTMISRLSRVKLHDYGCTLKAYRRDVVEGLEL
                     YGDAHRFLPALASRVGDRVTEIPVAHRPRVHGESKYGLSRVGKVFLDMLALPFMLRFF
                     NRPIRFFGTAGMLMGLAGSGLLVWMGVERFGLGRPIGGRPALLIGMLLVLAGVQLVTL
                     GLLGEAMTRAYYGGRGRHPYTVRRALVSCTPGHHTCSTPSSPSEITLP"
BASE COUNT          362 a          847 c          819 g          313 t
ORIGIN      
        1 aaatagagcc ggtacagcgt cgcgtacggc atcggatact cgagcgcccc taggtactcg
       61 cgccagcgca gcgcggacac cccgagcccg acgaggaaca gaagcgccgc gaacccgatc
      121 gggacgacct cggcctggcg aagctggcgg cccagctcgc ccaggtcggt acgggccacc
      181 aggatgccga agacgaccgc gcccaccacg acgcgcgcca cgaccttcag cgacttccgc
      241 acggcgcgat acattagcgc cccggtgcgc atcctcatcg tcaactacga atatccgccg
      301 ctcggcggcg gcggcggcgt tctcgcccga gcgctggttc gcgagctcgg ccgcgaccac
      361 gacgtgaccg tgctcaccag ccgcggcccc ggcctgcggc ccgagtcgtg ggaggacggc
      421 gcacggatcg tccgggtcag cgtcgtgcgc cgcaccgagc gcgagcgcgc ctcgatggct
      481 tcgctggtct ccttcggccc ggccgcgcgc cgcaccggcg cgaaactgat ccagcagcgc
      541 cgcttcgacc tggtgcacac cttcttcgcg gtcccctcgg gcccggcggg cgtcgcgctg
      601 gcccgccgcg gcggcgtgcc gcacctgctg accgtctgcg gcgccgacat ctacgacccc
      661 acccggccac tgtcgcccga acggttcgct ccggttcggg cgaccgtcaa gcgggtcgtg
      721 ggcgcatcgg agcgtgtgat cgcgatcagc aaggacctgg cggaccggac caagaagctg
      781 accggccgcc aggacgtcga catcgtcggc atcggggtcg agcccgcggt cctgccgccc
      841 cgctcgcggc cggccgacgg cgtgacccgc ttcgtgacgg tcgcgcgact ggttcagcgc
      901 aaggccctcg gcgcggtggt tcgctcggtg ccggacggat gccatctggt cgtggtcggg
      961 gagggccccg agcgggcgac gttggagcgc gcggcgacgc ccggacgggt gacctttgcc
     1021 ggcgcgctcg acgacgccgc gaaggcgcag gcgctcgccg acgcggacgc gttcgtgctg
     1081 gcctcgctgc acgaggcctt cggcatcgtc tacctcgagg cgatgcaggc cggcttgccg
     1141 gtgatcgcgg gcagcatcgg cggccagacc gacttcctga cgcacgatga gaacgcgctg
     1201 ctggttcccg ccggcgaccc cgtgcggctc ggtgaggcga tgaagcgaat ggccgccgat
     1261 ccggcgaagc gcgcgcgcat gggcctggcg gcgaaggaga ccgcacagcg gtacaccccg
     1321 cagtggatgg cgaaagagta cctggaccgc tacgagcggg cgctcgctac ggaagcgtga
     1381 tctcgctcgg cgacgacggc gtcgagcacg tgtggtgacc gggcgtgcac gacacgagcg
     1441 cgcggcgcac cgtgtaggga tgccggccgc ggccgccgta gtaggcgcgc gtcatcgcct
     1501 cgccgagcaa tccgagcgtt accaactgaa cgcccgccag caccagcagc atgccgatca
     1561 gcagcgccgg ccgcccgccg atcggccgtc cgagcccgaa tcgctcgact cccatccaca
     1621 ccagcagccc cgagccggcc aagcccatca gcatgcccgc agttccgaag aatcggatcg
     1681 gccgattgaa gaagcgaagc atgaacggca gcgcgagcat gtcgaggaac accttgccga
     1741 cccggctgag cccgtacttg gactcgccgt gcacccgcgg ccggtgcgca accgggatct
     1801 cggtcacgcg atcgccgacg cgcgaggcga gcgccggcaa gaaccggtgc gcgtcgccgt
     1861 agagctcgag cccctccacg acgtcgcggc ggtaggcctt gagcgtgcag ccgtagtcgt
     1921 gcagcttcac gcgcgacagc cgcgagatca tcgtgttggc gaccttgctc ggcagccgcc
     1981 gcgtccagag cgtgtccttt cgatcgacgc gccagcccga gaccacgtcg tagccttcgt
     2041 ccagcttcgc gagcatcttc gggatgtcgg ccgggtcgtt ctgcaggtcc gcgtccatcg
     2101 tgacaatgac gcggccgtgc gcgtaatcga agcccgcggc cagcgccgcg gtctgaccga
     2161 agttgcgcgt gaagcgcacc acgcggatcg tcggatcctt ctgatgcgcg tcctcgagca
     2221 cctgggtgga tccgtcggag gagccgtcgt cgacgaacac gacctcgctg ggcgggatcg
     2281 cacgccgcgc ggcgacgagc tcggagagca gcggcgccag cgagtcgcgc tcgttgtaga
     2341 c
//
LOCUS       DATLFO010000209         3071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_94344, whole genome shotgun sequence.
ACCESSION   DATLFO010000209 DATLFO010000000
VERSION     DATLFO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94344"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="VM841_08535"
     CDS             <1..1929
                     /locus_tag="VM841_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVL90265.1"
                     /translation="KIGFPVALIWGGVAGMLAAVVLGVVALRVRGLYLAVATLLFSWM
                     AEAFLFRFEPFTRHSTIEPQSTGIANSFPYFDWSDRRVYFYMAWAAVVVAVFLMANLR
                     DSKTGRAFFSVRGSEVAAASLGVDVVKTKLVAFAISGVLAGIAGGLMMAELGTISPDA
                     FKVDRSLFFLGIAVVGGLGSLGGAIAAGTVFAMLDEAFLRVKFLSGYLEVVAAALLAG
                     TLLLYRGGLAEVPRSLAPVLRPVSKALRPLNRALDAVWLASARSLRKMFASPPRPAAE
                     GASPAKPGLLARLTKGKAGGPPAVKAQEPLDLFTVGRIANGNGTHVHLEDEPLRTGMM
                     SVETSERTDRVVDGDLEIDEISAGSSRDWAALTLPTPPLPADRAVRTPLIEASGITVQ
                     FGGLTAVNNASLKVCEGEIVGLIGPNGAGKTTLFNSIAGLNVPTKGQVKLFGNDVTTW
                     PVHKRAQLGVGRTFQAIQMFGQLSVFDNLMVATDRHNPTGLFAHLTVSKASIGAEKAA
                     RAQVERALELLDLTSVADRTARDLPFGVLRMVELARALVTGAHLIMLDEPASGLDNKE
                     TDRLADILRFVRSLGVTLLLIEHDVRMVTSVSDYMYVLDRGNMLAEGTPDQVQRDERV
                     IAAYLGQQDEHETEEIQA"
     gene            1952..2845
                     /locus_tag="VM841_08540"
     CDS             1952..2845
                     /locus_tag="VM841_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90266.1"
                     /translation="MSLLEVETIDVYYGSVQALRGLSMRVEEGEMVALVGANGAGKTT
                     TLRTVSGLIAPRSGSIKFEGQEIGGIPAHKLVSRGIAHLPEGRDLFPTLSVEENLRYG
                     YWSRRKQKEKYSARLEQMFAYFPKLKERRKQASGTLSGGEQQMLGVARALMSSPKLFL
                     VDELSLGLAPIIVTQLFEILKDANREGTTIVLVEQFVHMALGNTQRAYVLAKGEVVME
                     NSSQALLDDPMLVASYLGEADLEAAYEESHPEAVPGQQDGSDDRPAPRKTSSRASSAA
                     TKTKKPVAKARKTVTKKSQGR"
     gene            2853..>3071
                     /locus_tag="VM841_08545"
     CDS             2853..>3071
                     /locus_tag="VM841_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90267.1"
                     /translation="MKRLRILPLALVIALAAAGCSSGSRSTSAGEREPGPGVSSTPSH
                     GDSEPSANPTGTAATGSPKPSSHGSDDDD"
BASE COUNT          503 a         1043 c         1011 g          514 t
ORIGIN      
        1 aagatcggct tcccggtggc gctgatctgg ggtggggtcg ccggcatgct cgccgccgtc
       61 gttctcggtg tcgtcgcact gcgtgttcgt ggcttgtacc tggccgtcgc gaccctgctg
      121 ttctcgtgga tggccgaggc gttcttattc cgattcgagc cgttcacccg ccactcgacc
      181 atcgagcctc agtccaccgg gatcgcgaac tcattcccgt acttcgactg gtccgaccgg
      241 cgcgtgtatt tctacatggc atgggccgcc gttgtggttg ccgtattctt gatggccaac
      301 ctgcgcgatt ccaaaaccgg ccgggcgttc ttctccgttc gcgggtccga ggtcgcggcc
      361 gcatcgctcg gcgtggacgt cgtcaagacg aagctggtgg cgttcgccat ctccggcgtg
      421 ctcgccggaa tcgccggtgg cctgatgatg gcggagctcg gcacgatcag tccggacgcg
      481 ttcaaggtgg accgctccct gttcttcctc gggatcgccg tcgtcggcgg cctcggcagc
      541 ctcggaggcg ccatcgcggc cggcacggtt ttcgccatgc tcgacgaggc ctttcttcgc
      601 gtgaagtttc tcagcggcta cctcgaggtc gtggcggccg cgctgctcgc cggcacgctg
      661 cttctgtacc gcggtggcct ggccgaggtc ccgcgctccc tcgcgccggt gctgcgcccg
      721 gtctccaagg ccctgcggcc gctgaaccgc gcgctcgacg cggtgtggct tgcctccgcc
      781 cgcagcctgc gcaagatgtt cgcttccccg ccccgtcccg ccgcagaagg tgcgtcgccg
      841 gcgaagcccg gactgctcgc gcgcctgacc aaggggaagg cgggggggcc gcctgcggtc
      901 aaggctcagg aaccgctcga cctgttcacg gtgggacgca tcgcgaacgg caacggcaca
      961 cacgtccacc tcgaagacga accgctgcgg accggcatga tgagcgtcga gacatccgag
     1021 cggaccgatc gtgtcgtcga cggcgacctc gagatcgacg agatctcggc cggaagcagc
     1081 cgggattggg cggcgctcac cttgcccact cccccgctgc ccgccgaccg cgccgtccgc
     1141 acgccgctca tcgaggccag cggcatcacc gttcagttcg gcgggctgac cgccgtcaac
     1201 aacgcgtcgc tcaaggtgtg cgaaggcgag atcgtcgggc tcatcggtcc caacggagcg
     1261 ggaaagacca cgctgttcaa ctccatcgcc ggtctgaacg tcccgaccaa gggccaggtg
     1321 aagctgttcg gcaacgacgt caccacctgg ccggtgcaca agcgcgcgca gctcggagtc
     1381 ggccgaacct tccaggccat ccagatgttc gggcagctct cggtgttcga caaccttatg
     1441 gtggcgaccg accggcacaa tccgaccggt ctattcgctc acctgaccgt ctccaaggca
     1501 tccatcggtg ccgagaaggc cgctcgcgcg caggtagaac gcgcgctcga actgctcgac
     1561 ctgacgtcgg tcgccgaccg cacggcgcgc gaccttccct tcggcgtgct ccgcatggtc
     1621 gagctcgccc gggcgctcgt gaccggcgcg catctgatca tgctcgacga gccggcgtcg
     1681 gggctcgaca acaaggagac cgaccggctg gccgacatcc tgcgcttcgt ccggtctctg
     1741 ggcgtcaccc tgcttctcat cgagcacgac gtgcgcatgg tgacgagcgt gtccgattac
     1801 atgtacgtgc tcgatcgcgg caacatgctc gcggaaggaa cgcccgacca ggtgcagcgc
     1861 gacgagcgtg tcatcgccgc gtacctgggt cagcaggacg agcacgagac cgaggagatc
     1921 caggcgtgat ccccaaccga ggtgaggtcc gatgagcctt ctggaagtgg agacgatcga
     1981 cgtctactac gggtcggtac aggcgctgcg cggcctgtcc atgcgcgtcg aggaaggcga
     2041 gatggttgcg ctggtcggcg cgaacggtgc cggcaagacc accacgctgc gaaccgtgtc
     2101 tggcctcatc gcgccccgtt cgggcagcat caagttcgaa ggtcaggaga tcggcgggat
     2161 tcctgcgcac aagctcgtct cacgcggcat cgcgcacctc ccggagggcc gcgatctttt
     2221 cccgacgctc agcgtcgagg agaacctccg gtacgggtac tggtcgcgtc gcaagcagaa
     2281 ggagaagtac tcggcccgcc tcgagcagat gttcgcgtac ttcccgaagc tcaaggagcg
     2341 ccgcaagcag gcctccggaa cgctctccgg cggcgagcag cagatgctgg gggtcgcacg
     2401 ggctctgatg tcgtcgccga agctgttcct ggtggacgag ctttccctcg gcttggctcc
     2461 gatcatcgtc acgcagctgt tcgagattct gaaggacgca aatcgtgagg gcacgacgat
     2521 cgtgctggtc gagcagttcg tccacatggc gctcgggaac acccagcgag cctatgtgct
     2581 cgccaaaggc gaggtcgtga tggagaacag ctcccaagcc ctgctcgacg atccgatgct
     2641 cgtcgcgtcc tacctcggag aggccgacct cgaggccgcg tacgaggaaa gccaccccga
     2701 ggcggtgccg ggtcaacagg atggctccga cgacaggccg gcgccgcgga agacttcgtc
     2761 gcgcgcctcg agcgccgcga cgaaaacgaa gaaaccggtc gcaaaagcac ggaagaccgt
     2821 gaccaaaaaa agccagggac gttgagatga tcgtgaagcg tcttcgcatc cttccattgg
     2881 cgctcgtcat cgcgctcgcg gcggcgggct gctcctccgg cagccggtca acgtcggccg
     2941 gcgagcgcga gccgggcccg ggcgtgtctt cgacgccgtc ccacggagat tccgaaccgt
     3001 cggcgaatcc gacagggacc gcagcgaccg gcagcccgaa gccgagctcg cacggctccg
     3061 acgacgacga c
//
LOCUS       DATLFO010000210         7410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_107021, whole genome shotgun sequence.
ACCESSION   DATLFO010000210 DATLFO010000000
VERSION     DATLFO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107021"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VM841_08550"
     CDS             complement(<1..269)
                     /locus_tag="VM841_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90268.1"
                     /translation="MHARRLRSLIALSAMTASLWVASPSSAAPSPIHVVEGSVAMMMR
                     FPADPTYTSWVKPARDVYNITGQTGQAGYAFDIQRRTWGGRFVLEL"
     gene            complement(352..1350)
                     /locus_tag="VM841_08555"
     CDS             complement(352..1350)
                     /locus_tag="VM841_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVL90269.1"
                     /translation="MNESRLARFLSTAVVLVLSASVVTAGIRSSARLSIQPPESSAAT
                     LSLKFIEPDPNAMPFPITDDVAPAPAPPPPAAKKGAVKAPIAMTAAHRAARQIDGDRA
                     SFHWALLIGINDYAGSTRDNIGSYQDAVALRELLLAQGWKPDHILLIGNRAATMAGVR
                     EGLAWLASKTDARSRVIFHYSGHEKPMRGDPDRDGEARDVALWLSDNSLMSDGELGVA
                     MGKVSASTMWINMAVCRAGGFDDPGMAAPGRVITYSSPESQLSYEDPEVNYSVFGYNM
                     LVRGMKMGLGDLNGDGVITVQEAFEYGRPLVLDRTGNRQRPFIKDMLGSGFDLRIV"
     gene            1676..2842
                     /gene="moeB"
                     /locus_tag="VM841_08560"
     CDS             1676..2842
                     /gene="moeB"
                     /locus_tag="VM841_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007444460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HVL90270.1"
                     /translation="MRPPPLVEPADSLTNEEIRRYSRHLIIPDVAMTGQKRMKNAKVL
                     CIGAGGLGSPVAMYLAAAGVGTIGLVDFDVVDETNLQRQIMHGTSDVGRPKLDSAEDR
                     IREINPNVKVVKHQTFVTSDNALDLFRDYDLIVDGSDNFPTRYLVNDACVLLGKPYVW
                     GSIYRFDGQLSVFWAEYGPCYRCLFPEPPPPGMVPSCAEGGVLGMLCGTIGSFQTTEA
                     VKLITGAGEPMIGRMLIYDALELSVRELKVRKDPECPVCGKNPTVTELIDYDAFCGVT
                     DDVAQFAAQLSITPKELEDLRAKEDVYLLDVRDPHEYEIVHLPDSHLIPMNEVLDRLS
                     EIPQSGNVIVMCKVGARSAQVLRYLHEVGLKNVKHLHGGLEAYAAQVDPGMPRY"
     gene            2854..3123
                     /locus_tag="VM841_08565"
     CDS             2854..3123
                     /locus_tag="VM841_08565"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVL90271.1"
                     /translation="MTVRVRVVVSGRVQGVFFRASTAEYARRAGVGGFVRNLADGRVE
                     AAFEGDEEAVEECLAWAGVGPSGAVVDSIEVVSEPPAGEVEFRIR"
     gene            3348..3530
                     /locus_tag="VM841_08570"
     CDS             3348..3530
                     /locus_tag="VM841_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90272.1"
                     /translation="MSKALFGYLGEPRAYVLLKQIAELKARVAELEKSLQSAEAEVMA
                     LRAIADAKMDEVKAMA"
     gene            3651..7034
                     /gene="smc"
                     /locus_tag="VM841_08575"
     CDS             3651..7034
                     /gene="smc"
                     /locus_tag="VM841_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HVL90273.1"
                     /translation="MFLKSLSLQGFKSFADKTTLEFTPGVAVIVGPNGSGKSNLSDAI
                     AWVLGEQGPKTLRGGKMEDVIFAGTPKRNGAGRAEVSLTIDNSAGVLPIEFTEVTITR
                     TIFRGGDSEYAINGTPCRLLDVQELLSDSGIGRELHTIVGQGQLDGVLQARPEERRAY
                     IEEAAGILKYRRRKERAVRKLERTDADIERLGDVIGELRRQIRPLEQQAEMAKRSAEI
                     QADLAEVTLRLWVHDYAAIAEEGDREAEARLTAEAEQLAARLQELAAAIEALENESAS
                     SSAAAEAALSTEYRLSSARERVAGLARLADERARHLDELAVRAPEGQAPPPELIAETE
                     AELAAIRVERERAEQQAREAEQEWRALTAAQEAAVKAREEIVHLQGERAALRAAVQAA
                     DEERQRLAERRAFLARRRQAREDEIKATADELRVLDDREGKFEAALDEARAALGEAQA
                     AANESAQRLRDLERSADALEARRAVLAEEAARGTPKDLIGRIPGLLGVLREIVRPVNG
                     WERAAQSALEPVAGAIVAARPEDAIQAIRTLKSAGGRGVFVSEGAGAAVPEGIRSAEE
                     AVEQAPDAVRALLRGIALADDLDGAWEIARANPGITVVTRDGDRIAGGVLSGGDPAGL
                     GRDVAAELAAVEAGLRENGERLASARMESERAAAAADEAASRVGELVRQGNEVDAELT
                     GVADHLAELEREDHAAQREDAMMAGRVQEMEERLVSDSEKLSAVESRLSRAVAEERPV
                     IDATQMAAVERRAAERSLHLGGLAERERTVARRVEDLKSRARRIAQEREEWERSREAR
                     IAASRKAAAIAAAAVMTRDRLEAWIEEARRARFGVEERRTRCAAELAEARRVRRETEQ
                     RAEEVREMAHRTDLARAERSHRLAAMIEKLRAEHDLSPEEAIGRAGTSHDDNVDELRR
                     RASVLERKLNMIGRVNPIAMEQYQGLVDRHAFLTEQVTDLRKSRKDLVSVVEEVDAKI
                     VEMFSAAFEDVKREFEQVFVRLFPGGEGKLALTDPSDLLESGVDVEARPGGKRVKRIS
                     LLSGGERSLVAIALLSSIFRARPSPFYLLDEVEAALDDVNLHRFLELVKEFKSASQIL
                     IVTHQKRTMEVGDILYGISMGNDGVTRVLAQRFDEPATL"
     gene            7179..>7410
                     /locus_tag="VM841_08580"
     CDS             7179..>7410
                     /locus_tag="VM841_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90274.1"
                     /translation="MDGPAYLFLYVGIAVVILIAVIVGFIRPGRRKPAAPSKAAPTEA
                     PAKPEAPPEPEAKPAPPKPTEPKAAPPAPPAAA"
BASE COUNT         1245 a         2413 c         2616 g         1136 t
ORIGIN      
        1 agctcgagca cgaagcggcc gccccaggtc cgcctttgaa tgtcgaaggc gtagccggcc
       61 tggccagtct ggccggtgat gttgtagacg tcgcgggccg gcttgaccca gctggtgtag
      121 gtcgggtcgg cggggaagcg catcatcatc gcgacggatc cttcgacgac gtggatcggc
      181 gagggcgcgg cagacgatgg cgatgcgacc cacagggacg cggtcatcgc gctcagagcg
      241 atgagcgagc gaagacgtcg tgcatgcatt ccgttgctct ccttctcgtc ccccctcggc
      301 ctgtggacgg gacgtcccgg ccgggctgcc tgcgatcgaa ctcgtcgagc gttaaacgat
      361 ccggaggtcg aatccgctcc cgagcatgtc ctttatgaac gggcgctggc ggttccctgt
      421 gcggtcgagc acgagcggcc ggccgtactc gaacgcctct tgaacggtga tgacgccgtc
      481 cccgttgagg tcgcccaggc ccatcttcat cccgcgaacc agcatgttgt agccgaacac
      541 ggagtagttg acctcggggt cttcgtagga tagttgcgac tcgggcgacg agtacgtgat
      601 cactcgcccc ggcgcggcca tgcctgggtc gtcgaagccg ccggcgcggc agacggccat
      661 gttgatccac atcgtcgagg ccgacacctt gcccatggcg acgccgagct ccccgtcgga
      721 catcaggctg ttgtccgaaa gccacagcgc cacgtcacgc gcctcgccgt cccggtccgg
      781 gtcgcccctc atcggcttct cgtgcccgga gtaatggaag atcaccctgg atcgcgcgtc
      841 ggtcttggat gcgagccacg ccagcccttc gcgcaccccg gccatcgtgg ccgcgcggtt
      901 gccgatcagc aggatgtgat cgggcttcca cccttgcgcg agcagcagct cgcgcagcgc
      961 cacagcatcc tgataggagc cgatgttgtc ccgtgtgctg ccggcatagt cgttgatccc
     1021 gatcaggagc gcccagtgga agctcgcccg gtcgccgtcg atctgacgcg cggcgcggtg
     1081 ggcggcggtc atcgcgatgg gcgccttgac cgcgcccttc ttggcggccg gcggaggagg
     1141 ggcgggcgcg ggagccacgt cgtcggtgat cgggaacggc atcgcgttgg ggtccggctc
     1201 gatgaacttc agcgagagcg tcgcggccga gctttccggc ggctggatcg acaggcgggc
     1261 cgagctccgg atccccgccg tcacgacgga ggcggacagg accaagacca cggccgtgga
     1321 cagaaatcgt gcgaggcggc tctcgttcat cgtcgagttc gacagttcgt cggggcttgg
     1381 gcgggctcct gccgcaacgt ccgcggcatg cgggaacgtc ccggttgcgg ccagtttacg
     1441 cttccccggc ctgccgggcc ggcgggggca ggcagggaat cggttctttg gatacccccg
     1501 cgggaatggg gctcgcttcc ggcgaattcg caagcacgat cgggcccggg ctcggtcgaa
     1561 gcggtggaag gaattccagg tattccgatg gcgttggtat gattccaccg ttcgcacgag
     1621 ggagacagtg gcaatgcccg accagaccca gcctcagatg cttcagaacg gcggcctgcg
     1681 gccgccgccg ctcgtcgagc cggccgactc gctgaccaac gaagagattc gccggtactc
     1741 gcgccatctc atcattcccg acgtcgcgat gacgggccag aagcgcatga agaacgccaa
     1801 ggtcctgtgc atcggcgcgg gcgggctcgg ctcgccggtt gcgatgtatc tggccgcggc
     1861 gggcgtgggc acgatcgggc tggtcgactt cgacgtcgtg gacgaaacca atcttcagcg
     1921 ccagatcatg cacggcacct ccgacgtcgg ccgtccgaag ctggacagcg ccgaagaccg
     1981 gatccgcgag atcaacccga acgtgaaggt ggtcaagcac cagacgttcg tcacgagcga
     2041 caacgcgctg gatctgttcc gcgactacga cttgatcgtc gacggctccg acaacttccc
     2101 gacgcgctat ctggtcaacg acgcgtgcgt gctgctcggc aagccgtacg tgtggggctc
     2161 gatctatcgt ttcgacggac agctgtcggt cttctgggcc gagtacggcc cctgctatcg
     2221 ctgcttgttc cccgagccgc cgccgccggg aatggtcccg tcgtgcgcag aagggggcgt
     2281 gctgggcatg ctgtgcggga ccatcggatc gttccagacc accgaggccg tgaagctgat
     2341 caccggcgcg ggcgagccga tgatcggacg gatgctgatc tacgatgcac tcgagctgag
     2401 cgtgcgggag ctgaaggttc gcaaggaccc ggagtgcccg gtgtgcggca aaaacccgac
     2461 cgtcaccgag ctgatcgact acgacgcgtt ctgcggtgtc accgacgacg ttgcgcagtt
     2521 cgccgcgcag ctgtcgatca caccgaaaga gcttgaagac cttcgcgcga aagaggacgt
     2581 ctacctgttg gacgttcgcg acccgcacga gtacgagatc gtgcacttgc cggactcgca
     2641 cctgatcccg atgaacgagg ttctggatcg gctgtcggag atcccgcaga gcggcaacgt
     2701 gatcgtgatg tgcaaggtgg gcgcgcgctc ggcgcaggtc cttcgatact tgcacgaggt
     2761 cggcctcaag aacgtcaagc atctgcacgg ggggctcgag gcctacgccg cccaggtcga
     2821 tcccggcatg ccgcgctact gattgggcgg gccgtgaccg ttcgcgttcg ggttgttgtc
     2881 tccggccgcg tgcaaggcgt gttcttccgc gcatccaccg ccgagtacgc acggcgcgcc
     2941 ggcgtcggtg ggttcgttcg caacctggcc gacgggcggg tcgaggccgc gttcgaaggc
     3001 gatgaggaag cagtcgagga gtgcctcgcg tgggcagggg tcggcccttc cggggccgtc
     3061 gtggactcga tcgaggtagt ctccgagcct ccggcggggg aggtcgagtt ccggatccgg
     3121 tgatcaagtg tggattcttc ttccatgaag aatcagcaca aaatcggggc gaaatggatg
     3181 aatcctcgct gacttgccaa atctttccat agaaaagttg gcgtcaggac ttctcacggt
     3241 ccgggggctg agttatagtc ctcttgtgaa cccgggtggc gatcctgcct gggcgcgcac
     3301 gaggaagaaa acaagcggcc aaccgaaccc ttaaaggggg tcgagacatg tcgaaggcgc
     3361 tcttcggata cctcggagag cctcgcgcgt acgtgttgct gaagcagatc gcagagctga
     3421 aggcacgtgt cgccgagctg gagaagtcgt tgcagtccgc cgaagccgaa gtgatggcgc
     3481 tccgcgcgat cgcagacgcg aagatggacg aagtcaaggc aatggcgtag aggaagcggg
     3541 ggtcatcccg aagaccccga gataaacgcc cctcgcaggg gcgtttttct tttgcgttta
     3601 tccatagcat cgatccgcgg acacgcgcgg acgccgtgct agcatcggcc gtgtttctca
     3661 aatccctgtc ccttcaaggg ttcaagtcgt tcgccgacaa gaccacgctc gagttcacac
     3721 cgggcgtcgc cgtcatcgtc ggccccaacg gatccggcaa gtcgaacctg tctgatgcga
     3781 tcgcatgggt gctcggagag cagggaccca agacgctgcg cggcggcaag atggaagacg
     3841 tcatcttcgc cggaacgccc aagcggaacg gcgccggccg cgcggaagtc tcgctgacga
     3901 ttgacaactc ggccggggtg ctgccgatcg agttcaccga ggtcacgatc acccgaacca
     3961 tcttccgcgg cggcgatagc gaatacgcga tcaacggaac accgtgccgc ctgctggacg
     4021 tgcaggagct gctgtccgac agcggcatcg gccgtgagct tcacaccatc gtcgggcaag
     4081 gccagctcga cggggtgctg caggcgcgtc ccgaagagcg gcgagcctac atcgaagagg
     4141 ctgcgggaat tctgaagtac cggcgccgca aggaacgcgc cgtccgcaag ctcgagcgca
     4201 ccgacgcgga catcgagcgc ctcggcgacg tgatcggcga gctgcgccgc cagatccgtc
     4261 cgctcgagca gcaggccgag atggccaagc gaagcgccga gatccaggcc gatctcgccg
     4321 aggtcacgct gcggctgtgg gtgcatgact acgccgcgat cgccgaggaa ggcgaccgcg
     4381 aggcggaggc acgtctgacg gccgaggccg agcagctcgc cgcccggctc caggagctcg
     4441 ccgccgcgat cgaagcgctc gagaacgagt ccgcgtcgtc gtcggcggcg gccgaggcgg
     4501 ccttgtcgac cgagtatcgc ctgtcgtcgg cacgcgaacg ggtcgccggc ctggcgcgtc
     4561 tcgccgacga gcgcgcgcgg cacctcgacg agctcgccgt gcgcgctccc gaagggcagg
     4621 ctccgccgcc ggagctgatc gccgagaccg aagccgagct cgccgcgatc cgcgtcgagc
     4681 gcgagcgcgc cgagcagcag gcgcgcgaag ccgagcagga gtggcgcgcg ctcaccgcgg
     4741 cacaggaagc ggccgtgaag gcacgcgaag agatcgtgca tctgcaaggg gagcgagccg
     4801 cactgcgcgc tgcggtgcag gccgccgacg aggagcggca gcggctcgcg gagcgtcgcg
     4861 cgttcctggc acgccggcgc caagcacgcg aagacgagat caaggccacc gcggacgagc
     4921 ttcgcgtgct cgacgatcgc gaaggcaagt tcgaggccgc gctcgacgaa gcgcgcgccg
     4981 cgctcggcga ggcgcaggcc gcggccaacg aaagcgcgca gcggcttcgc gatctggagc
     5041 gcagcgccga tgcgctggag gcgcgccgcg ccgtgctcgc cgaggaagcc gcgcgaggaa
     5101 ccccgaagga cttgatcggc cggatccccg gcctgctggg cgtgctgcgc gagatcgtgc
     5161 ggccggtaaa cggatgggag cgggcggcgc agtcggccct cgagcccgtg gccggcgcca
     5221 tcgtcgccgc acggcccgag gacgcgatcc aggccatccg gacgctcaag agcgccggcg
     5281 gacgaggcgt gttcgtctcg gagggcgccg gcgcggccgt gcccgaagga attcgcagcg
     5341 ccgaggaagc cgtcgagcag gctccggacg cggtgcgcgc gctcttgcgc ggcatcgcct
     5401 tggccgacga tctggacggt gcgtgggaga tcgcccgggc caatcccggc atcaccgtcg
     5461 tcacgcgcga cggcgaccgg atcgcgggcg gggtgctgtc cggcggcgat ccggccggtt
     5521 tgggccgtga cgttgcagcc gaactcgcgg ccgtcgaggc cgggcttcgt gagaacggcg
     5581 agcggctcgc gagcgcccgc atggaatcgg agcgcgccgc cgcagcggcc gacgaggccg
     5641 cgtcccgcgt cggcgagctg gtgcggcaag gcaacgaggt ggacgcggag ctgaccgggg
     5701 tcgccgatca tctcgccgag ctcgagcgcg aggatcacgc cgcgcagcgc gaggacgcga
     5761 tgatggcggg ccgcgtgcaa gagatggagg agcggctcgt gtccgacagc gaaaagctct
     5821 ccgccgtgga gtcgcgcctt tcccgggccg tcgccgaaga acggccggtg atcgacgcca
     5881 cgcagatggc cgcggtcgag cgccgggccg cggagcgatc ccttcacctc ggcggtctcg
     5941 ccgaacgtga acgcaccgtc gcgcgccgcg tcgaggatct gaagtcccgt gcgcgtcgca
     6001 tcgcgcagga gcgcgaggag tgggagcgct cgcgtgaggc ccggatcgcg gcatcgcgca
     6061 aggctgcggc gatcgccgcg gccgcggtga tgacgcgcga ccgcctggaa gcctggatcg
     6121 aggaggcgcg ccgtgcgcgc ttcggggtcg aggagcgccg cacgcgctgc gccgccgagc
     6181 tcgccgaagc gcgacgggtc cgacgcgaga ccgagcagcg cgccgaagag gtgcgtgaga
     6241 tggcgcaccg gaccgatctc gcgcgtgccg agcgctcgca ccgcctcgcc gccatgatcg
     6301 aaaagctccg tgccgagcac gacctttcac cggaggaagc gatcggcagg gccggcacct
     6361 cccacgacga caatgtcgac gagcttcggc ggcgtgcttc ggtgctcgag cgcaagctca
     6421 acatgatcgg ccgcgtgaac ccgatcgcga tggagcagta tcaggggctg gtggaccgcc
     6481 acgcgttcct caccgagcag gtgacggacc ttcggaagtc ccgcaaggac ttggtgtccg
     6541 tcgtcgaaga ggtggacgcc aagatcgtcg agatgttcag cgcggccttc gaagacgtca
     6601 agcgcgagtt cgagcaggtc ttcgtgcgcc tgttccccgg gggcgaagga aagctggcgc
     6661 tgaccgatcc gtcggacctg ctcgagtcgg gtgtggatgt ggaggcccgg ccgggcggca
     6721 aacgcgtcaa gcgcatctcg cttctgtcgg gcggtgagcg gtcgctggtc gcgatcgcgc
     6781 tgctgtcgtc gatcttccgc gcccggccct cgccgttcta tctgctcgat gaggtcgagg
     6841 ccgcgctcga cgacgtgaac ctgcatcgtt ttcttgagct ggtgaaggag ttcaagagcg
     6901 cttcgcagat cctgatcgtg acgcatcaga agcgcacgat ggaggtcggc gacatcctct
     6961 acggcatctc catgggcaac gacggcgtga cccgtgtcct cgcccaacgc ttcgacgagc
     7021 ccgccacgct atagcgatcc ggtggcatcg cccgccgggg cccctctgcc ggcggtcgat
     7081 gcatgcgcac aaggtttgct ggtcatatga ctccgctatt cggagtcata tgaccaacaa
     7141 acatccgtga gcctcgtcgg gtaaggtacc cgctcgtcat ggacggaccc gcataccttt
     7201 tcctgtacgt cggcatcgcc gtcgtcatcc tgatcgcggt catcgtcgga ttcatccggc
     7261 cgggccgacg caagcccgcg gcgccgagca aggcagcccc caccgaggcc ccggcgaagc
     7321 ccgaagcgcc gcccgagccg gaagcgaagc ctgcgccgcc caagcccacg gagccgaagg
     7381 ccgctccgcc ggcgccgccc gcggccgctc
//
LOCUS       DATLFO010000211        10888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_86444, whole genome shotgun sequence.
ACCESSION   DATLFO010000211 DATLFO010000000
VERSION     DATLFO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86444"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VM841_08585"
     CDS             <1..775
                     /locus_tag="VM841_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90275.1"
                     /translation="FEQKSLIEVSDGSARVVAVGEADLNAWRRHISDGTTVRMRALED
                     GTTAELSAGRLTPAMAVARAHMEVAGLAAFCCRKLYDAGRTRDFTRLMKLLKDTYFGS
                     SMRIQVKGTPELNEYLRPEEGRPLGTGELIKAMDDFRASGDEPGTRCQMTYGLLSEFA
                     HPAMRASSTFAEVLSEHETGWYIQYREEDRLGEQSARMALEILLDNMRIGHGSAALLN
                     LAGVVQKGATFLLRTPSPDEVGDVYEHLLQQPPDGSTPA"
     gene            complement(1160..2074)
                     /locus_tag="VM841_08590"
     CDS             complement(1160..2074)
                     /locus_tag="VM841_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL90276.1"
                     /translation="MDPALWIGVTDTDWFTLLRAIPDLDEANFWQPSPTPVRSLQPGA
                     PFLFKLHRDNRIVGGGFFAKWTPLRVSMAWDAFGIKNGVASLSEMRARIERYRRTPSA
                     PHEDYEIGCILLEELFFLPDSERVVIPDWQPNIVRGKGYPLAQEPGRSIWAQTQAVLT
                     GTRVGHVAEPAERYGVPQLVRPRLGQGSFRVMVTDAYARRCAVTGEKVLPVLQAAHIR
                     PYADGGEHRVDNGLLLRSDLHTLFDRGYLTVTPEHRVEVSRRLETEFNNGREYLSLHG
                     TQVALPQDHDDIPAKEFLEWHNENRFVA"
     gene            complement(2111..2356)
                     /locus_tag="VM841_08595"
     CDS             complement(2111..2356)
                     /locus_tag="VM841_08595"
                     /inference="COORDINATES: protein motif:HMM:NF022671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HVL90277.1"
                     /translation="METRGRSITIFEHRPPWRADFGPDWTKRSIAQFRYDPGTMTWSL
                     WWSDRNGKWLRVPDAPPAGDVDPLLKIVDENRTGAFE"
     gene            complement(2489..3571)
                     /locus_tag="VM841_08600"
     CDS             complement(2489..3571)
                     /locus_tag="VM841_08600"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HVL90278.1"
                     /translation="MQQLEEARRALNLFAGQAAEPGDAPKYAALARIAAEDDGIAELL
                     AAAPATQRHATLFFAALHYTMLRNPQDPLAEWFSVNEDSADPEVLLRGFCGRHHDALR
                     DLIMFRKTQTNEVQRSSHLVLMLSQVAQQASRPIALVEPGAAAGLNLLLDHYFYDWGT
                     AGTMGDAGAFVKLSADVLGSLPSIATTLPVVRTRIGIDLAPVNLEDDDAVLWLRACVW
                     AEHRRRGKVLEQAIDFARSRPPAVIAGDAVELLPDVLDQLSSADVAVCVMHSAFLPYL
                     SEAQRQAFVERLVDASHRYPVHWIVSEGWGLVVPMFQLEEPPRPPGADYSLLGVICTL
                     DKGRVVTRLVLRAAPHGRWLQWLADD"
     gene            complement(3962..4384)
                     /gene="vsr"
                     /locus_tag="VM841_08605"
     CDS             complement(3962..4384)
                     /gene="vsr"
                     /locus_tag="VM841_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HVL90279.1"
                     /translation="MSDWLTPEQRQRNMSAIRSKGTRPELALAELLQELFPRRKIIQH
                     AELPGRPDFYLPGVRAAIFVDGCFWHSCPTHGRAPATNTDYWSRKLDRNSQRDRDVDR
                     ELRKSGVKPIRIWEHDLRGRGVIAGRTKVRRRLTRLAR"
     gene            4544..5584
                     /locus_tag="VM841_08610"
     CDS             4544..5584
                     /locus_tag="VM841_08610"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HVL90280.1"
                     /translation="MGMLTSLEICTGAGGQALGLEQAAFHHLALVEIEEPACRTLKLN
                     RPEWPVVRADVRDFHARGLRGQVDLLAAGVPCPPFSVAGKQLGADDERDLFPEVIRLA
                     KETNPRAVFVENVPGLLESRFDGYRTQVLEQLDSLGYEGEWRKFNASDFEVPQLRPRA
                     LLVAAKRPTWKRFTWPEPSKKQPLTVGQALLGEMKRNGWLGAEEWAAGANSVAPTLVG
                     GSKKHGGPDLGPSRARARWAELGVNGKVLAQEPPEQNFVGLPHLTVKMAALLQGFPQD
                     WVFFGPKTVAYRQVGNAFPPPVAKAIGIALAKALTTQASEDRVEYGQLIELPRPKTHS
                     LPDEDTAARDVR"
     gene            complement(5605..6303)
                     /locus_tag="VM841_08615"
     CDS             complement(5605..6303)
                     /locus_tag="VM841_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HVL90281.1"
                     /translation="MPTFHWKDPNAGAAKRVAHYLATEVGEGNVFKKSVLREIIPNTE
                     QVDRRMRDLRKVHWKIRTYRDRPGLRPEELLLEKIGHRVWEDGYKWPQDGLSTAVRRK
                     VLERDGPRCMVCGIDFGAEYIDHPGITARPTIGHIVAKERGGTNELDNLRPECHLCNE
                     QSRNLTASPIDIGLLQRRILQLGRAEKRKLLGWMRAERRSFTDTELLWAQVCRLPRPE
                     REAIQRDLESAVEL"
     gene            complement(6454..7200)
                     /locus_tag="VM841_08620"
     CDS             complement(6454..7200)
                     /locus_tag="VM841_08620"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HVL90282.1"
                     /translation="MSEVHRITERQMRRQPLVSQYLENLSSDVFELYQDVIKRYARGR
                     HGIYALYRNDRLYYVGLAQNMPSRLRTHLRDRHQGLWNRFSIYLTINDGHMRELEALL
                     LRITPTPGNRMKGKFAKAENLRTNLAKEIRSHQQAELDELLGRKPQRRRNTRTPRQNG
                     SIPIRAQYKGVKYRARLRADGTIRYEGEIFSSPSAAGKAVIGRAVNGPAFWKYQRAPG
                     DWVPLKQREASQSARRRRRRTTRPRSRSAA"
     gene            7749..8093
                     /locus_tag="VM841_08625"
     CDS             7749..8093
                     /locus_tag="VM841_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90283.1"
                     /translation="MTFFRDVLAMTDEQIETIRAQPSWPTRIAAAHTIRRESQAERTT
                     RLDARMLGRITVPVLLLVGEDSDEFFVIDVAAVAAALPDARISTIPGQTHMADVFAPE
                     LVAERVLPFVLA"
     gene            complement(8090..8500)
                     /locus_tag="VM841_08630"
     CDS             complement(8090..8500)
                     /locus_tag="VM841_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL90284.1"
                     /translation="MANERQQANEQRIRDLFAVFMGGDVEGASHFFAEDAVFWYEGPG
                     PIHGDWQGRDGVKAFWDAQSRGSAGGLRPELVDLVASERNAFLLVRFPRTDGGGSWHR
                     VVVYEFDGDLIVNARVFESDPDAAVAYFSQGSGG"
     gene            complement(8713..9993)
                     /locus_tag="VM841_08635"
     CDS             complement(8713..9993)
                     /locus_tag="VM841_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL90285.1"
                     /translation="MQVTVRPYRPEDLGSVLQLWEAVEGKLGAGLLSMDQLVDLIGSG
                     SAAAFVGERDGRLLGAAVGAVSGAIGWLYRVVLDDAAAADDALAASLIARTEASLLEA
                     GARKVVAPVVPGRGMHPHLARAGYAELDDRRLVEKRLAAALAAPSTLAELGGRMIDPG
                     LWDRLRGMEQAKQIIERRVILPLAEPELAERHGVSPPKAIVLFGPPGTGKTTFAKGIA
                     SRLGWPFIELEPGELAGGGADRQAKALADTFLRIRELPSAVIFVDEVEDLASIRHEER
                     RTNPSVTNEFMKQIPRLRQVPHHLLVCATNWVGRLDPAFLRPGRFDFILPVGPPDAAA
                     RRSIWERYVSEITDEVVALDTLVEASDLFTAADIEFAARKAAQGAFEREHFDGIARRA
                     GTADFLSAIADTRPTLNAEMIEHFAQDTERFARD"
     gene            complement(10276..>10888)
                     /locus_tag="VM841_08640"
     CDS             complement(10276..>10888)
                     /locus_tag="VM841_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90286.1"
                     /translation="LAALLIAQRAATLRDQQRRQDDLVRVFAEGQQQLEEIAAIRQEV
                     SGLLVSLRRQLDAEQMAALGGTMPYGAWAERWLAYIQAPTSRSNMIAMVAWQLAEGTS
                     ARWNPLATTWKMPGSTQFNGHRVQNYVSLEQGMEATRKTLSRPRLGYEPILANLHAGA
                     AAMDTAKAINASRWCRGCADGQYVIELIPIVERYYDKYAKRRG"
BASE COUNT         1975 a         3565 c         3392 g         1956 t
ORIGIN      
        1 cttcgagcag aagtctctca ttgaggtcag cgacggatca gcaagggtcg tcgcggttgg
       61 cgaagcagat ctcaacgcat ggcgccgtca catatcagat ggaactaccg tccgcatgcg
      121 cgcgctcgaa gacgggacga cagcggagct ctccgccggg cggctaacgc ccgcaatggc
      181 cgtagcccgg gctcacatgg aggtcgccgg ccttgcggcc ttctgttgcc gcaagctcta
      241 cgacgcgggc aggaccagag atttcactcg tctgatgaag ctgctcaagg acacatactt
      301 cggttcgagt atgcgcatcc aggtgaaagg cacaccggag ctgaatgaat accttcggcc
      361 agaggaaggg cggccgctgg gaacgggtga actcatcaag gcgatggatg atttccgcgc
      421 ttcaggcgac gagcccggta caaggtgtca gatgacctat gggttgttga gcgagttcgc
      481 ccacccggcc atgcgcgcga gctccacgtt cgccgaagtc ctttccgagc atgagacggg
      541 ttggtacatc caataccgcg aagaggatcg cctgggtgag cagagcgccc gcatggcgct
      601 tgagattctc ctcgacaaca tgcgaatcgg gcacggctca gccgccctcc tgaatctcgc
      661 aggcgttgtg caaaagggag cgacgttcct gttgagaact ccctcgcccg acgaggtcgg
      721 ggatgtgtac gaacatctgc ttcaacagcc gccggacgga tcgacgccgg cctgatccct
      781 ggcgactaaa tggcgactac acgtggaccc gaaggggtct cggcgggatc accggggaat
      841 ggcgccacgg ccaacgacgt gcaggaacgc gaattgccgc tggtaggatg atcgtcgaaa
      901 ggagagggga ctcgcgttca aatcccccct tcccgactga aaaacaagtc tctgactagg
      961 gaaaacgcgg atttcacatg atcacgtgtg catccgtgtg cattttccga acgggaaatc
     1021 gcggggtatt tcgccgattg ggtgtccagt ccgctcgagg atgcccttcg ccgggtcttt
     1081 ctcgcaccct tcccctacgc tgatttcagg cgttgaagtc ggttcttggc cttccgtgat
     1141 gagcgggctc gaccaaccct caggcgacga accggttctc gttgtgccac tccaggaatt
     1201 ccttcgccgg gatgtcgtcg tgatcctgtg ggagtgcgac ctgcgttccg tgcagggata
     1261 gatactcgcg accattgttg aactcagtct cgagccgtct gctcacttcg actcggtgct
     1321 caggcgtaac cgtcagatac ccgcgatcga acagcgtgtg cagatcgctt cgcagcaata
     1381 gcccgttgtc gacgcggtgc tctcctccgt cggcgtaggg ccggatgtgc gcggcttgca
     1441 gcacaggcaa cactttctcg ccggtaaccg cgcaacgacg ggcgtaggca tcggtaacca
     1501 tcacgcggaa tgaaccctga cccagacgtg gccggacgag ttgtggcacc ccgtaacgtt
     1561 cagcgggttc cgccacgtgc cccacgcggg tcccagtgag cacggcttgc gtctgcgccc
     1621 agatcgagcg gcccggctct tgggcgagcg gatagccctt cccgcgaacg atgttcggct
     1681 gccagtctgg aatcacgacg cgttcggagt ccggcaggaa gaagagttct tcgagaagga
     1741 tgcagccgat ctcgtaatct tcgtgaggcg ccgaaggcgt tcgccggtac cgctcgatcc
     1801 gcgcgcgcat ctcgctgaga gacgcgacgc cgttcttgat accgaacgca tcccaagcca
     1861 tcgatacgcg gagcggcgtc cacttggcga agaaaccacc accgacgatc cggttgtcgc
     1921 gatgcagctt gaacaggaat ggcgcgccgg gttggagtga ccggaccggc gtcgggctgg
     1981 gctgccagaa gttcgcctca tccaggtcgg ggatggcgcg caacagagtg aaccaatcgg
     2041 tatcggtcac tccgatccac aacgccggat ccatggcggc gattgtagat tcacgcggcc
     2101 gtgagagcct ctactcgaac gcgcctgtcc ggttctcgtc gacgatcttg agcagcgggt
     2161 caacgtcgcc ggcgggcggc gcgtcgggaa cacgcagcca cttgccgttg cggtcggacc
     2221 accacagcga ccacgtcatc gttcccgggt cgtagcggaa ctgcgcgatg ctgcgcttcg
     2281 tccaatccgg gccaaagtcc gcgcgccaag gcgggcggtg ctcgaagatc gtgatcgacc
     2341 ggccgcgggt ttccaccttc cacacaagct ggtcgcggac gcgctcgggg atcttccgac
     2401 tggggaaaga cccggtccgc aggtcaccac agaacaccag ttcgcttccg tgggacgcct
     2461 gcctcgtgtg ggagcttgtc cccctccttc agtcatctgc cagccactgg agccaacgac
     2521 cgtgtggagc cgcacgcaag accagccgcg tgaccacccg ccctttgtcc agcgtgcaga
     2581 ttactccgag cagcgaatag tcggcacccg gcggtcgtgg gggctcctcg agctgaaaca
     2641 tggggacgac aaggccccag ccttcactca cgatccagtg aaccgggtat cggtgcgaag
     2701 catcgacgag ccgttccacg aacgcctggc gttgcgcctc cgagagatag ggcaggaatg
     2761 ccgaatgcat gacgcagacg gcgacgtcgg cgctcgaaag ctgatcgaga acgtccggca
     2821 aaagctccac cgcatccccg gcgatgacgg ccggaggcct cgatcgcgcg aaatcgattg
     2881 cctgttcgag aacctttcct cgccgccgat gttctgccca tacgcacgcg cgaagccaaa
     2941 gtaccgcatc gtcatcctca agattcacgg gcgcaaggtc gatcccgatc cgcgtccgca
     3001 ccacgggaag cgttgtcgca atcgagggaa gagatccgag cacgtcggcg gaaagcttca
     3061 cgaatgctcc cgcgtcaccc atggtgcctg cagttcccca gtcgtagaag tagtggtcga
     3121 ggagcaggtt caaccctgct gcggcaccgg gctctacgag tgcgatcggg cggctcgcct
     3181 gctgtgccac ctgggaaagc atgaggacaa ggtgcgagct tcgctgcact tcattcgtct
     3241 gggtcttgcg aaacataatg agatcgcgaa gcgcatcatg atgccggcca cagaatcccc
     3301 gcagcagaac ttccggatcg gcactgtcct cgttcacgct gaaccactcg gcaagcggat
     3361 cctgaggatt ccgcagcatg gtgtagtgaa gcgcagcgaa gaacagcgtc gcatgtcgct
     3421 gcgtcgccgg tgcggcggcg agcaattcgg cgataccgtc gtcttcggcc gcgatccggg
     3481 ccagcgcggc gtatttcggt gcatctccgg gctcggccgc ttgacctgca aaaagattca
     3541 gtgcccgacg agcctcttcg agttgctgca cccggcctcg aaccatcctc gaacaatagg
     3601 cgcggtcggg cgacgcggct aattcggcca agtcctcggg tgccgacggc ttgtcgcggc
     3661 cgggatgtgc gagtcgagtg tctcagcacc ggaggcccgg ctggccacga tcggcccttc
     3721 tttttgccgg agagcgccgc catcggtccc tgcacggcct gacgggctac gctgacgtcg
     3781 ccgtgcctga cgaacgccgc ggcgccctgt acggcgtggc cgcctatctc atgtggggac
     3841 tcgttcccct ctactggcat ggctaggcgg ctaacgcggt gctggtggcg ccgaagtgtc
     3901 caccatctct gatggagttg ccagcgtctg gcgagcggga cggcgcgaag gccccacagc
     3961 atcatcgagc caaacgtgtc aggcggcgtc gaaccttcgt ccgtccagcg atgactccgc
     4021 gtcctcgcag gtcatgttcc cagatgcgta tcggcttgac cccactcttt ctcagttccc
     4081 gatcgacgtc tcggtcgcgc tgactattcc ggtcgagctt tctgctccag tagtcggtgt
     4141 tcgtggccgg cgcgcgccca tgggtcgggc atgagtgcca gaaacaaccg tcaacgaaga
     4201 tggccgcccg cacgccgggc aggtagaagt ccggtctgcc cggcagctca gcgtgctgga
     4261 tgattttgcg cctggggaag agctcctgga ggagttccgc taaggcaagc tcgggtcgcg
     4321 tccccttaga acgaatagcg ctcatgttgc gctgacgctg ctcaggggtc agccaatcac
     4381 tcatacgttt ccgtccttac cgcaccgggg cggacgccgc tgcgctgtct atcccctgtg
     4441 gctcgacgtc gagatcgttt ctgtctcacc tctttagtat cgtccacgct gccttcccgc
     4501 cgcccggggc ctccgggact tcggttcaag gacgtggtac acaatgggga tgctgacctc
     4561 cctcgagatc tgcacgggcg ccggagggca ggctctgggc cttgagcagg cggccttcca
     4621 tcatcttgct ctcgttgaga tcgaggaacc ggcgtgccga acgcttaagc tgaacaggcc
     4681 ggaatggccg gtagtgcggg ccgacgtccg cgacttccat gcccgcgggc tgcgcgggca
     4741 ggtcgacctg ctcgccgctg gagtgccctg cccgccgttc tctgttgctg gaaagcaact
     4801 cggcgccgac gatgaacgcg acctgttccc cgaggtcatc cggctcgcca aggaaacgaa
     4861 tccccgtgcc gtcttcgttg aaaacgtacc tggcttgctt gagtcgaggt tcgatggcta
     4921 caggactcag gtcctggagc agctggattc actcggatac gaaggcgagt ggcggaagtt
     4981 caatgcgagt gacttcgagg tgccacagct tcggcctagg gcacttctgg tcgctgccaa
     5041 acgcccgacg tggaaacgct tcacctggcc cgagccttcg aagaagcaac ccctaaccgt
     5101 cggacaagct ttgctcggcg agatgaaacg aaatggctgg ctgggcgctg aggagtgggc
     5161 agccggcgca aacagtgtcg cgccaaccct cgttggtggt tcgaagaagc acgggggtcc
     5221 tgacctaggc ccgtcacgcg ccagagcacg gtgggccgag ctcggcgtca acggcaaagt
     5281 cctcgcgcag gaaccgccag agcagaactt cgtcgggctc ccacatctga cggtgaaaat
     5341 ggcagccttg ctccagggct tcccacagga ttgggtgttc ttcgggccga aaaccgtagc
     5401 ctaccgacag gtggggaatg cattcccgcc accagtggcg aaagcgatcg gaatcgctct
     5461 tgccaaagcg ctcaccaccc aggcgtcaga agacagagtc gaatacggcc aactcattga
     5521 gcttcctcgg ccgaagacac actccctgcc cgatgaagac accgcagccc gcgacgtgcg
     5581 ttagctaggg ccgtcggggc cggctcacag ttcgactgcg ctttctagat cccgctgaat
     5641 ggcctcacgt tcggggcggg gaaggcggca gacctgcgcc cacaagagtt ccgtgtcggt
     5701 gaacgagcgg cgctccgcac gcatccagcc gagcagtttg cgcttctcag cacggcccag
     5761 ctgcaggatt ctccgctgga ggagaccaat gtcgatagga gacgctgtca ggtttcgcga
     5821 ctgctcattg cagagatgac actccggccg taagttgtcg agctcgttgg ttccgcctcg
     5881 ttctttggcc acgatgtgcc caatcgttgg ccgggctgta atgcctgggt gatcgatata
     5941 ctccgcgccg aaatcgatgc cgcacaccat gcatcgcggt ccgtcgcgct cgagcacctt
     6001 cctgcgaacc gcagtgctta gcccgtcctg cggccacttg tatccgtctt cccagacacg
     6061 gtggccgatc ttctccagca gaagttcctc cggacgcaag ccaggccggt cgcggtaggt
     6121 ccgaatcttc cagtgcacct tccgcaagtc acgcatgcgc cggtcgacct gctccgtgtt
     6181 tggaatgatc tcgcggagga ccgacttctt gaagacattg ccttcgccta cttcggtggc
     6241 caagtagtga gcaacacgct tggccgcacc ggcgttcgga tccttccagt gaaacgtcgg
     6301 cattcaatgc tcctaacttg acccctcgga gttctcccaa gaatagcgtc aggctgcccg
     6361 gcgggacgat gccgaaccag agagtcaacg gcacccggca tgggcagccg atctgtgcgt
     6421 agcacggtgg cgatccagac tttttgttgt gccttaggcg gcgcttcggg atctcggtcg
     6481 cgtggttcgg cgacggcgac gccttgcaga ttgggatgcc tctcgttgct tcaacggcac
     6541 ccagtcgccc ggggcacgct gatacttcca aaaggccggg ccgtttaccg cgcggccaat
     6601 aactgccttg ccggccgcag aaggcgacga gaagatctct ccctcgtatc tgatcgtccc
     6661 gtcggcacgc aggcgtgccc ggtacttgac acccttgtac tgggctcgga tcgggatcga
     6721 tccgttctgg cgaggagtgc gagtgttccg ccggcgctgg ggtttgcgcc cgagcagctc
     6781 gtccaattcg gcttgctggt gggagcgaat ttccttggca agattagtcc gcaggttctc
     6841 cgccttggca aacttgccct tcatccgatt ccccggggtg ggcgtgatcc gcaacagcag
     6901 ggcctcaagt tcacgcatat gaccatcgtt gatggtgagg tagatgctga agcgattcca
     6961 caggccctga tgacggtctc tgaggtgagt ccgaagccgg gacggcatgt tctgagccag
     7021 acccacgtag tacaagcggt cgttcctgta aagcgcatag atgccgtgcc tgcctcgcgc
     7081 gtatctcttg atgacgtcct ggtagagctc gaagacatca ctcgaaagat tctcaagata
     7141 ctgagacaca agcggttggc ggcgcatctg acgctccgtt atccggtgga cttcactcac
     7201 caagttgtca ctcatcgcaa gatcggcccc ttctacggcc ccctttaggc taggaagtcc
     7261 agaattcgct cccgccagga gccgcccacg cagatgcggg ggcccggccg tcattacgtt
     7321 gaccccaggg ggttgagcgt ggtgctcgaa gctcgcaaag ccctgcgatc tactccccag
     7381 ccgccgcctt caacacacgt cacccgaccg gagggggccg gcgatggctg agcaacacgg
     7441 cgtcgtgcgt tcgcgcgacg gaacgcagat cgcttacttc acgacgggaa ccggcccgcc
     7501 cgttgtgctc gtcgcaggga cgaccggcga ccacaaccgg tgggtaccgc tctttcgcca
     7561 tctcgagcag cacctcacaa tccacgcgat ggaccgccgc ggccgcggag ccagcggcga
     7621 cagccccgac taccacatcg agcgcgaata cgaagacgtc gccgcggtca tcgaggacgt
     7681 tggcggcaag agcggatcaa cggttgcgct ctacggacac tcgttcggcg gattgtgcgc
     7741 gctcggcggt gacgttcttc cgcgacgtgc tggcgatgac cgacgaacag atcgagacga
     7801 ttcgtgcgca gccgtcgtgg ccaacgcgca tcgccgccgc gcatacgatc cggcgcgaga
     7861 gccaagccga acgcacgacc cgcctcgacg cccggatgct cggtcgcatc acggtgccgg
     7921 tgctgctgct cgtcggcgaa gatagcgacg aattcttcgt catcgacgtc gcagccgtcg
     7981 cggcagcgtt gcccgacgcg cggatctcga cgatccccgg tcagacccac atggccgacg
     8041 tgttcgcacc ggaactggtc gcggaacgcg tgctgccgtt cgtcctggcc tagccgccgc
     8101 tgccttgcga gaagtaggcg acggcggcgt ctggatcgga ttcgaacacc cgcgcgttca
     8161 caatcagatc tccgtcgaat tcgtagacga cgacgcgatg ccagcttcct ccaccatccg
     8221 tacgcgggaa ccgcacgagc aggaaagcat tgcgttcact cgcgacgaga tcgaccagct
     8281 cgggccgcaa tccgcccgcc gacccacggc tttgcgcatc ccagaacgcc ttgacgccgt
     8341 cgcgaccttg ccagtcgccg tggatcggcc cggggccctc gtaccagaac acggcatctt
     8401 cggcgaagaa atgtgaggcg ccctcgacat cgccgcccat gaacacggcg aacaagtcac
     8461 ggatacgctg ctcgttcgct tgctgccgtt cattcgccat tcgagactcc tttccaccct
     8521 tacccgatga cacaacccca cgcatcggtc cggtcagggc gcgttccgaa catcgacctc
     8581 catgaccatt cacccccgga acaactatcg cggccggcct cctatgcaac cgatatcgga
     8641 tccaccgacc tccgcggccc atcctccacg tgacggatcc tgctagagcc cgcacggacc
     8701 agaagccggc cctcagtcgc gggcgaatcg ttcggtgtct tgagcgaaat gttcgatcat
     8761 ctcggcgttc agcgtggggc gtgtgtcggc gatcgcgctg aggaaatcgg cagtcccggc
     8821 acggcgggcg atcccgtcga aatgctctcg ttcgaacgca ccttgggccg ctttgcgggc
     8881 tgcgaactcg atgtcggcgg cggtgaacag gtcgctggcc tcgacgagcg tgtccaacgc
     8941 aaccacctcg tcggtgatct cggagacgta ccgctcccag atcgagcggc gcgcggccgc
     9001 gtcgggcggg ccgacgggca ggatgaaatc gaaccgcccg ggcctcagga acgcgggatc
     9061 gagccggccc acccagttgg tcgcgcacac cagcaggtgg tgcggcactt gccgcaaacg
     9121 cgggatctgc ttcatgaact cgttggtgac gctcggattc gtgcggcgct cctcgtggcg
     9181 gatggacgcg agatcctcga cctcgtccac gaagatgacg gccgacggca gctcgcggat
     9241 gcgtaagaac gtgtcggcga gcgcttttgc ctgccggtcg gcgccgccgc cggcgagctc
     9301 accgggctcg agctcgatga acggccagcc gagccgcgac gcgatgcctt tcgcgaacgt
     9361 cgtcttgccg gtgcccggcg gcccgaacag cacgatcgcc ttcggcgggc tgaccccgtg
     9421 ccgttcggca agctccggct cggcgagcgg caagatcacg cgccgctcga tgatctgctt
     9481 cgcctgttcc atgccgcgga gccgatccca aaggcccgga tcgatcatgc ggccacccag
     9541 ctcggcgagc gtgctgggcg cggccaacgc ggccgcaagc cgcttctcca ccagacggcg
     9601 atcatccaac tcggcataac cggcccgcgc cagatgcgga tgcataccgc gacccggcac
     9661 gaccggcgcg acaaccttgc gtgcgcccgc ctcgagcaga ctcgcctcgg tgcgcgcaat
     9721 cagcgacgcg gcaagcgcat catcggcagc ggccgcatcg tcgaggacca cgcgatacag
     9781 ccatccgatc gcacccgaga ccgcaccgac cgcggcaccc agcagccgcc cgtcgcgttc
     9841 gcccacgaac gccgccgccg aacccgaccc gatcagatcc accagctgat ccatcgacag
     9901 caggccggcg ccgagcttgc cctcaaccgc ctcccacagc tgaagcaccg aacctagatc
     9961 ctcgggacga tacggccgca cggtcacctg catgacgctc ctcgctcggt caccccacca
    10021 taccgaggcc gctctccctc gtagcgggga aatgcggggt gaacgtggca tcaaccgtga
    10081 acaggtcgtc tcatcgccag tgatgatggc ttcgcgcaga gcgatcaccg ggtgaaccga
    10141 tggtccccgg ccgcttgaac cttgatcacg cgggcgtttg gaaccgtggc ggcccatggg
    10201 cacgtgtccg gcagtcgagc gggcagcacc cgcgtgccaa ccgagccagc aaggagtcga
    10261 cttcgttcac gatggctacc cgcggcgttt cgcgtacttg tcgtagtagc gctcgacgat
    10321 cgggatgagc tcgataacgt actggccgtc ggcgcatccg cgacaccagc ggctggcgtt
    10381 gatggctttc gcggtgtcca tcgcagcagc accggcatgc aagttcgcga ggatcggctc
    10441 gtagccgagc cgcggccggc tcagcgtctt gcgcgttgcc tccatgcctt gctcgagcga
    10501 cacatagttc tggacgcggt gcccgttgaa ctgtgtcgag ccgggcatct tccaggtcgt
    10561 tgcgagcgga ttccagcggg cgctcgttcc ttcggcgagt tgccaggcca ccatcgcgat
    10621 catgttgctg cggctcgtcg gagcctggat gtaggcgagc cagcgttccg cccacgcgcc
    10681 gtagggcatc gtgccgccga gcgccgccat ctgctcggcg tcgagctgcc ggcgcagcga
    10741 cacgagcagg ccggacacct cttgacgaat cgcggcgatc tcctcgagct gctgctggcc
    10801 ttcggcgaag acgcgcacga gatcgtcctg gcggcgctgc tgatcgcgca gcgtcgcggc
    10861 gcgctgcgcg atcagcagcg ccgccagg
//
LOCUS       DATLFO010000212         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_58264, whole genome shotgun sequence.
ACCESSION   DATLFO010000212 DATLFO010000000
VERSION     DATLFO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58264"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VM841_08645"
     CDS             <1..1852
                     /locus_tag="VM841_08645"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HVL90287.1"
                     /translation="HYTAAAFVVGALFSAVAGYIGMMTSVNANVRTAEAARGGIDPAL
                     KVAFRGGSVTGMAVVGLALLGVAGFTWLYQDALGLSLRDTVVALVGFGFGASLISLFA
                     RVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMGADLFETY
                     AVTAIAAMLLGLLLFDGDSTFVLYPLALGAVAIAASIVGAFFVSAKDGRIMVGLYKGV
                     AATAVLALGAFAAVTWAMFGDVDATFDRFYGDPGTLTPARLFGAALAGMVVMVGMIVI
                     TEYYTSTSFRPVRKIANASVTGAGTNLISGLAVGLESTFVPVLLIATAMLVSYELAGL
                     YGIAVAAVAMLSTTGIIVSLDSYGPITDNAGGIAEMAGLPKEVRKHTDALDAVGNTTK
                     AVTKGYAIGSAALAALALFADYNERLVEAGEEIVFELVDPYVLVGLFIGAVLPFLFSA
                     FLMEAVGKAAFSIVSEVRRQFREIKGIMEGTAKPDYAACVDIVTKAALREMVVPGIMA
                     VAAPLLVGFLLGPAALGGLLVGVIASGLLVALFMANAGAAWDNAKKYIESGHHGGKGS
                     DAHKAAVVGDTVGDPTKDTAGPAINALIKVINTVSLIFAALIVGYHWLPA"
     gene            2019..>2437
                     /locus_tag="VM841_08650"
     CDS             2019..>2437
                     /locus_tag="VM841_08650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006333.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HVL90288.1"
                     /translation="MVYRIVEMQDTIRIPPDMLGDDLEHVMEELVHKSFEGKLSRLNG
                     ITVLTQNVKPLGEGLMIHGDGDIYQKVKFEALVFKPEIQEVVDGIVAEVAEFGAFVHI
                     GPLDALVHMSQVMNDYVSVDTNNERLVGKESKKEVGVG"
BASE COUNT          365 a          835 c          840 g          397 t
ORIGIN      
        1 gcactacacc gcggccgcct tcgtcgtcgg cgccctgttc agcgccgtcg cgggctacat
       61 cggcatgatg acgtccgtga acgcgaacgt gcgcacggcg gaagcggcgc gtggcggcat
      121 cgacccggcg ctgaaggtcg cgttccgcgg cggaagcgtc accggcatgg ccgtcgtcgg
      181 cttggcgctg ctcggcgtcg ccggctttac gtggctctac caggacgcgc tcgggctctc
      241 gctgcgcgac acggtcgtcg cgctcgtcgg gttcggcttc ggcgcgtcgc tcatctccct
      301 cttcgcgcgt gtcggtggcg gcatctacac gaaggccgcg gatgtggggg cggacctcgt
      361 cggcaaggtc gaggcgggca tcccggagga cgacccgcgc aaccccgccg tcatcgcgga
      421 caacgtgggc gacaacgtcg gcgactgcgc cggcatgggc gccgacctgt tcgagacgta
      481 cgcggtcacc gcgatcgcgg cgatgctgct cggcctgctc ctgttcgacg gggacagcac
      541 gttcgtgctc tacccgctcg cgttgggcgc ggtggccatc gcggcgagca tcgtgggcgc
      601 gttcttcgtc agcgcgaagg acgggcgcat catggtgggc ctctacaagg gcgtcgcggc
      661 caccgccgtg ctcgccctcg gcgcgttcgc cgccgtgacg tgggcgatgt tcggcgacgt
      721 cgacgcgacg ttcgaccgct tctacggcga ccccggcacg ctcacgcccg cccgcctctt
      781 cggcgcggcc ctcgccggca tggtggtgat ggtcgggatg atcgtcatca ccgagtacta
      841 cacgagcacg agcttccgcc ccgtgcggaa gatcgcgaac gcctcggtca ccggtgcggg
      901 caccaacctc atctccggcc tcgcggtcgg cctcgagagc acgttcgtgc cggtcttgct
      961 catcgccacg gcgatgctgg tcagctacga gctcgcgggc ctctacggca tcgcggtcgc
     1021 ggcggtcgcg atgctgagca cgacgggcat catcgtgagc ttggacagct acggccccat
     1081 cacggacaac gccggcggca tcgcggagat ggcgggcctg ccgaaggagg tgcgcaagca
     1141 cacggacgcc ctcgacgccg tcggcaacac gacgaaggcg gtcacgaagg ggtacgcgat
     1201 cggctccgcg gccctcgccg cgctcgcgct cttcgcggac tacaacgagc gcctggtcga
     1261 ggcgggcgag gagatcgtgt tcgagctcgt cgacccgtac gtgctggtcg gcctgttcat
     1321 cggcgcggtg ctgccgttcc tgttcagcgc gttcctgatg gaggccgtgg gcaaggccgc
     1381 gttcagcatc gtcagcgagg tgcgccgcca gttccgcgag atcaagggca tcatggaggg
     1441 caccgcgaag cccgactacg ccgcctgcgt cgacatcgtc acgaaggcgg cgctgaggga
     1501 gatggtcgtg cccggcatca tggcggtcgc cgccccgctg ctggtcgggt tcctgctcgg
     1561 ccccgccgcg ttgggcggac tgctcgtggg cgtcatcgcg tccggcctcc tggtcgcgct
     1621 gttcatggcg aacgccggcg cggcctggga caacgcgaag aagtacatcg agagcggcca
     1681 ccacggcggc aagggcagcg acgcgcacaa ggcggccgtg gtcggcgaca ccgtcggcga
     1741 cccgacgaag gacaccgcgg gccccgccat caacgcgctc atcaaggtca tcaacaccgt
     1801 gtcgctgatc ttcgcggcgc tcatcgtggg ctaccactgg ctgccggcct gacgcggtca
     1861 cgttcctctt cccatcctct ccttccggcg cccgaatttc cgcgcggccg ccgaaaagct
     1921 ttataaggaa ccccctgtct cgccgcccgt cctggacccg ccctagcttt tctcatctcg
     1981 tgccgcggtg aggaaggccc gtccgccctg gtgtcatcat ggtctaccgt atcgtcgaaa
     2041 tgcaggacac catccgcatc ccgcccgaca tgctcgggga cgacctcgag cacgtgatgg
     2101 aggagcttgt ccacaagagc ttcgagggca agctgagccg cctgaacggc atcaccgtcc
     2161 tgacgcagaa cgtgaagccg ctcggcgagg ggctcatgat ccacggcgac ggcgacatct
     2221 accagaaggt gaagttcgag gcgctcgtct tcaagccgga gatccaggag gtcgtcgacg
     2281 gcatcgtggc ggaggtcgcc gagttcggcg ccttcgtgca catcgggccc ctggacgcgc
     2341 tcgtgcacat gagccaggtc atgaacgact acgtgtcggt cgacacgaac aacgagcggc
     2401 tcgtgggcaa ggagtcgaag aaggaggtcg gcgtcgg
//
LOCUS       DATLFO010000213         7964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_48776, whole genome shotgun sequence.
ACCESSION   DATLFO010000213 DATLFO010000000
VERSION     DATLFO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48776"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="proC"
                     /locus_tag="VM841_08655"
     CDS             <1..732
                     /gene="proC"
                     /locus_tag="VM841_08655"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HVL90289.1"
                     /translation="PQEVIVTVRRQERADEMQRIYGIVATFDNAQAVRDAEIALIAVK
                     PQDMEVLLEQIRPASTPDHLLISVAAGITTAFIERRLPDGVPVVRTMSNVPAVVDEAM
                     TAVSAGAHASEKHLAVAEEIWSHVGRVVRLAEKHLDAVTATSGSGPAYFALLAEAMID
                     ACILLGLSRDVATDLIVQTMLGSAKMLREGKMHPVELRELVTSPGGTTISAVRELENA
                     GVRAAFLNAIHAACRRATELASGHE"
     gene            713..1270
                     /locus_tag="VM841_08660"
     CDS             713..1270
                     /locus_tag="VM841_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90290.1"
                     /translation="MRADTSSKAPRRAFTTRDGRGGLVRPARRSDAAACVAIVDEATR
                     MQPRTIMTMTDEVWSVREWRRRMLGQQDRGITLVAEVDGRVAGLLGAVRGDRPVTAHG
                     AEFGITVAEEYRGAGVGRAMLEALEDWARARGVWRLVLGVFENNTNARALYESLGYEV
                     EGVERQAVAFPDVTVNVLRMSKFLR"
     gene            1267..1956
                     /locus_tag="VM841_08665"
     CDS             1267..1956
                     /locus_tag="VM841_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90291.1"
                     /translation="MTSLIIPGRFNGPPGSANGGYCCGAVAGALGLGDGEAATVTLRS
                     PPPLDTPMRVEPDGDGIRVVSGSTLVAEAHRSELDLTVPEAVSFAEASRAQILPAEHP
                     FPGCFVCGPDHESGLRIFVGRVPGRKMHAGPWIPDPDLADNGAIARPVVWASLDCPGG
                     WVRGRDRALLGRLEAVVRSAPAPGDRCVAMAWLIDEPSERRWIAGTALYGENGVLHGA
                     ARATWVVPRPA"
     gene            2050..3537
                     /locus_tag="VM841_08670"
     CDS             2050..3537
                     /locus_tag="VM841_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL90292.1"
                     /translation="MSGDTNRLFIDGEWVPASSGDTFENRNPADSDDLIGIYAAGGAA
                     DIDAAAAAAKAAYPAWMKTPAPARADYVLRVGLMLEERKEELAVLMSREMGKTLRESR
                     ADVQEAIDFAYYMVGEGRRLFGVTTRSELPNKFSMTVRHPIGVAGLITPWNFPIAIPT
                     WKIFPALIAGNTVVFKPAEDTPASALELVKMFEQAGLPKGVLNLVTGMGETAGAALVE
                     HPDVRCISFTGSLEVGRMINARCGELMKRCSLELGSKNCLIVMPDADIDLAVEAGAWG
                     AFATSGQRCTATSRMAVHGDVLDRFADQLVARVSEYKVGPGTDPSTDLAAVINRKQRD
                     RILEYIGVGQSEGAKLVAGGQALEEGAYGKGNFVAPTIFREMTWDMRIAQEEIFGPVT
                     GLVEIGSLDEAIEVANSTEYGLSCSIYTRDITNTFEAIDQLEFGVVYINAPTIGAEVQ
                     LPFGGMKSTGNGHREAGPVALDEFTEWKSVSVDYSGKLQKSQGID"
     gene            3542..4699
                     /locus_tag="VM841_08675"
     CDS             3542..4699
                     /locus_tag="VM841_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HVL90293.1"
                     /translation="MSERCAVVWDDEVIAYDFGPQHPLKPVRVKLTVELSRAYGILDA
                     PNVALHRPVHATRDELELVHAPEYIDAVEQISLVADRAFGNYGWGIGIGDNPAFRGMH
                     DASALVAGGSLLAARLVHDGNVEHAFNPAGGLHHAMRDRASGFCIYDDPAIAIAWLLR
                     GGHERIVYVDVDVHHGDGVQAAFYDDPRVMTISLHESGEYLFPGTGFPGETGAECTSV
                     NVALPPMTGDDAYREAFDDIVPPLVEAFRPSILVTQLGCDTHATDPLAHLALTTRSYR
                     HLASSFHDLAHRVCDGRWLAVGGGGYQIYTVVPRAWTLYAAELAEVELDPSIPLSWID
                     TARSLGAQSPPAVLDDPEVTVFSGRLDEAREGARTAVAATKRAVFGHYGLT"
     gene            complement(4807..5712)
                     /locus_tag="VM841_08680"
     CDS             complement(4807..5712)
                     /locus_tag="VM841_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL90294.1"
                     /translation="MRDRESLVGGGLIALASLQFGIVVVVGTQLLRGGLSVTAMLAIR
                     FAVAGAVLLMIQAGRRKPLLPAAGERLWLALLGLAGYAVEAALFFAAIRYGTATAVTL
                     LFFTYPVLVALISMAIGRGTPGRLVAGAMAAAIAGAAIVVAAGGAVAIRPAGIALALA
                     SAVMFAAYLTGVDHVMRRTPPLTASAWVAIWASIGLFASLTIGSGRHAPGPAEEWIRV
                     AIMGLATGGAFVCLMAGLRMLGPVRTSVVAALEPLSSAILATIVLDEPLSAATAAGGL
                     LILGGATTASLARREPVEVTESEIP"
     gene            5777..6580
                     /locus_tag="VM841_08685"
     CDS             5777..6580
                     /locus_tag="VM841_08685"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HVL90295.1"
                     /translation="MTPSERKALRESLLAEMIAGDTLTPRESVVGNAEKLAAGDRDKF
                     FGFDFGVLDAQSVIDDVAAVCGCSNDLSERTGPGVIDVERTLDALHAVADRLRAARSR
                     GERVMFATGHPTGLMPVYMEIARTLAANGCKLVTPRSGEKLRQAFHGSVSSAWEMARR
                     RGNRVRYLDGVGALSDGAHLYHTHESWPMEMLLDGIDPPDLVIADHGLAGAAIVRGVE
                     TLAFADVNDPALSVAKRRGMTEVVVPLDDNRDPERYAAMTAFLAAGAGA"
     gene            6782..6982
                     /locus_tag="VM841_08690"
     CDS             6782..6982
                     /locus_tag="VM841_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL90296.1"
                     /translation="MASPVSPSGPRFLTALEVAERMRVSKMTVYRLIRSGKLPAVRVG
                     KSFRVREEDVDKYLDSSYVRAV"
     gene            7137..>7964
                     /locus_tag="VM841_08695"
     CDS             7137..>7964
                     /locus_tag="VM841_08695"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HVL90297.1"
                     /translation="MAVALGGMDSGKTTFCKMAAEVAVRVGRKVAYLDTDVGQTTIGP
                     PATIGMKYLEHEDDLKPEALAHPDAMYFVGSVTPQGHLLPIVVGASKMAEQARAHGAD
                     IILVDTTGLIGGTLGQILKYHKIEVLRPDIVVGFQRGEELEPILGAIRRAFPLTVETL
                     PVPDEVKPTNADDRITHRQERLRAAFEPGVHHWKVRMGIFIPNVPPDLDPALLDGMLV
                     GLEDGTGQCMGLGILEHRDGSLLLRSTADEGAKALRIGSTRVSPEYMTSRVDLRDLFV
                     "
BASE COUNT         1284 a         2730 c         2747 g         1203 t
ORIGIN      
        1 ccgcaagagg tcatcgtgac ggtgcgccgg caagagcgcg ccgacgagat gcagcggatc
       61 tacggcatcg tggccacctt cgacaacgcg caggcggttc gcgatgccga gatcgcgctg
      121 atcgccgtca agcctcagga catggaggtc ttgctcgagc agatccgtcc cgcatcgacc
      181 ccggatcatc tcctcatcag cgtcgcggcc ggtatcacca cggcgttcat cgagcggcgg
      241 ctgcccgacg gggttccggt ggtgcgcacg atgtccaacg tgcccgccgt cgtcgacgag
      301 gcgatgacgg ccgtctccgc cggagcgcac gcgagcgaaa agcatctcgc ggtcgccgag
      361 gagatctggt cgcacgtcgg ccgggtcgtg cgcctcgccg agaagcatct cgacgccgtg
      421 accgccacgt ccggctccgg tcccgcctac ttcgcgctgc tcgccgaggc gatgatcgac
      481 gcgtgcattt tgctgggctt gtcgcgcgac gtggcgaccg acttgatcgt gcagacgatg
      541 ctcggcagcg ccaagatgct tcgcgagggc aagatgcacc cggtcgagct gcgagagctc
      601 gtgaccagcc cggggggaac gacgatcagc gcggtccgcg agctcgagaa cgcgggggtg
      661 cgtgccgcgt tcctcaatgc catccacgcg gcctgccgga gagcgaccga gcttgcgagc
      721 ggacacgagt agcaaggcgc cgcgacgtgc cttcaccacg cgcgacggcc gcggcggtct
      781 cgtccggccc gcgcgccgca gcgatgcggc cgcctgcgtg gcgatcgtcg acgaggcaac
      841 caggatgcag ccgcgcacca tcatgaccat gaccgacgag gtgtggagcg tgcgggagtg
      901 gcggcgacgg atgctcggcc aacaggaccg ggggatcacg ctcgtcgccg aggtcgatgg
      961 aagggtggcc gggctgctgg gtgccgttcg cggcgaccgg ccggtaaccg cgcacggcgc
     1021 agagttcggc atcaccgtgg ccgaggagta tcgcggcgcc ggcgtcggcc gggcgatgct
     1081 cgaagcgctc gaggactggg cgcgcgcccg cggcgtgtgg cgtctcgtgc tcggagtctt
     1141 cgagaacaac acgaacgcgc gcgcgctcta cgagtcgctc ggctacgagg tcgaaggggt
     1201 cgagcggcaa gcggtggcgt ttcccgacgt gaccgtgaac gtgcttcgga tgtcaaagtt
     1261 ccttcgatga cgtccttgat catccccggc cgcttcaacg gcccgcccgg ctccgcgaac
     1321 ggaggatatt gctgtggggc cgttgccgga gcgctcgggc tcggcgacgg cgaggccgcc
     1381 accgtcaccc ttcgctcgcc gccgccgctg gacacgccga tgcgcgtgga gcccgacggc
     1441 gacggaatac gcgttgtgtc gggctcgacc ctggtggccg aagcgcatcg gtccgagctc
     1501 gacctcaccg tgccggaggc cgtttcgttc gcggaggcgt cgcgcgcgca gatcctgccc
     1561 gccgagcatc ccttccctgg ctgcttcgtg tgcggccccg accacgaatc cggcttgcgg
     1621 atcttcgtcg ggcgagtccc cggccggaag atgcacgccg ggccgtggat cccggacccg
     1681 gatctggccg acaacggcgc gatcgcccgt ccggtggtgt gggcgtcgct cgattgcccc
     1741 ggcggctggg tacgcggccg cgaccgggcc ctgctggggc ggctcgaagc cgtggtgcgg
     1801 agcgctcccg ctcccggcga ccggtgcgtg gcgatggcgt ggctgatcga cgagccttcg
     1861 gagcgtcgtt ggatcgccgg cacggcgctc tacggtgaga acggtgtctt gcacggcgcg
     1921 gcgcgcgcga cctgggtcgt gccgcgtccg gcctagggcg tgccgcgtcc ggcccgcgca
     1981 tccgtggcgc ccgtccgggg aagtaggatc aggccagttc ccgtgaccac gaaagaggtg
     2041 acctgaagca tgagcggcga cacgaatcgt ctgttcatcg acggcgaatg ggttcccgca
     2101 agcagcggcg acacgttcga gaatcgaaac cccgcggatt ccgatgacct gatcggaatc
     2161 tatgcggccg gcggcgccgc cgacatcgac gcagccgcgg ccgcggccaa ggcggcgtac
     2221 ccggcgtgga tgaagacgcc ggcgcccgcg cgcgccgact acgtcctgcg cgtcggcttg
     2281 atgctcgagg agcgcaagga agagctcgcc gtcctgatgt cgcgcgagat gggcaagacg
     2341 cttcgcgagt cgcgcgctga cgtccaggaa gccatcgact tcgcgtatta catggtcggc
     2401 gaaggccgcc gcctgttcgg ggtgacgaca cgaagcgagc tgcccaacaa gttttcgatg
     2461 acggtgcggc atccgatcgg cgtggcggga ctgatcacgc cgtggaactt tccgatcgcg
     2521 atcccgacgt ggaagatctt tcccgcgctc atcgccggca acacggtcgt gttcaagccg
     2581 gcagaggaca cgcccgcatc ggcgctcgag ctcgtcaaga tgttcgagca ggcgggtctg
     2641 cccaagggcg tgctgaatct ggtcaccggc atgggcgaga cggccggcgc tgcgctcgtc
     2701 gagcatccgg acgtgcgctg catctccttc accggctcgc tcgaggtcgg ccgaatgatc
     2761 aacgcgcgct gcggcgagct gatgaagcgc tgctcgctgg agctcggatc gaagaactgt
     2821 ctgattgtca tgccggacgc cgacatcgat ctcgccgttg aggccggcgc gtggggtgcg
     2881 ttcgccacca gcggccagcg gtgcaccgcg accagccgca tggccgtgca cggcgacgtg
     2941 ctggaccggt tcgccgacca actcgtcgcg cgcgtttccg agtacaaggt gggcccgggc
     3001 accgacccat ccaccgatct tgccgcggtc atcaaccgga aacagcgcga ccggatcctg
     3061 gaatacatcg gcgtcggcca gagcgagggc gccaagctcg tcgcgggcgg ccaggcgctc
     3121 gaagagggcg cgtacggcaa gggtaacttc gtcgctccga ccatcttccg cgagatgacg
     3181 tgggacatgc gcatcgcgca ggaagagatc ttcggtccgg tgaccggcct ggtcgagatc
     3241 ggatcgctcg acgaggcgat cgaggtcgcg aactcgaccg agtacgggct gtcgtgctcg
     3301 atctacacgc gcgacatcac caacacgttc gaggccatcg accagctgga gttcggtgtc
     3361 gtctacatca acgcgcccac catcggcgcc gaggtgcagc tgccgttcgg cggcatgaag
     3421 tccaccggca acggccatcg cgaagcgggc ccggtcgctc tcgacgagtt caccgagtgg
     3481 aagtccgtca gcgtggatta cagcggcaag ctgcagaagt cccaggggat cgactagcgc
     3541 cgtgagcgag cgctgcgccg tcgtatggga cgacgaggtc atcgcctatg acttcggccc
     3601 tcagcatccg ctcaagccag tccgggtcaa gctgaccgtc gagctgtcgc gtgcgtacgg
     3661 gattctggat gcgcccaacg tggcccttca ccggcccgtg cacgcgacgc gcgacgagct
     3721 cgagctggtt cacgcgcccg aatacatcga cgcggtcgag cagatcagcc tcgtggccga
     3781 tcgcgcgttc ggcaattacg gctggggcat cggcatcggc gacaacccgg cgtttcgagg
     3841 catgcacgac gccagcgcgc tcgtcgcggg aggatcgctc ctcgcggccc ggctcgtgca
     3901 cgatggcaac gtcgagcacg cgttcaatcc cgcgggcggc cttcatcatg cgatgcgtga
     3961 ccgggccagc ggattctgca tctacgacga tcccgcgatc gcgatcgcgt ggcttcttcg
     4021 cggcgggcat gagcgcatcg tctacgtgga cgtggacgtg catcacgggg acggcgtcca
     4081 ggccgccttc tacgacgacc cccgcgtcat gacgatctcg ctccacgagt cgggcgagta
     4141 tctgttcccg ggaacgggct tcccaggcga gaccggtgcc gagtgcacca gcgtcaacgt
     4201 cgcgctcccg ccgatgaccg gcgacgacgc ctaccgcgag gccttcgacg acatcgttcc
     4261 cccgctcgtg gaggcgttcc ggccctcgat cctggtcaca cagctcggct gcgacacgca
     4321 tgccaccgat ccgctcgcgc atctggcgct caccacgcgc tcctatcgtc atctcgcatc
     4381 ctcgttccac gatctggcgc atcgcgtctg cgacggccgc tggctcgcgg tgggcggcgg
     4441 cggctaccag atctacacgg tggtgccgcg cgcatggact ctctacgccg ccgagctcgc
     4501 cgaggtcgag cttgatccgt cgatcccgct gtcgtggatc gacaccgcac gatcgctcgg
     4561 cgcgcagtcc cccccggcgg tgctcgacga ccctgaggtg accgtcttct ccggccggct
     4621 ggatgaggcg cgggagggag cgcgcacggc cgttgccgcc acgaagcgcg cggtgttcgg
     4681 tcattacggc cttacctgac tggcgcgttt cgagccccag gcccgcggga atcatgccgc
     4741 aacaccacgg gcgcttcgct tcacgccggg tgcgcgcacc gcgtccgata ccggtgagtg
     4801 ccgcttctag gggatctcgg actccgttac ctcgaccggc tcgcgcctgg ccaggctcgc
     4861 ggtcgtcgcc ccgcccagga tcagaagccc gcccgctgcc gtggccgcgg aaagcggctc
     4921 gtcgagcacg atcgtcgcca ggatcgccga cgacaacggt tccagtgccg ccacgaccga
     4981 cgtccggacg ggccccagca tccgcaggcc ggccatgagg cagacgaacg ctcctcccgt
     5041 cgcaaggccc atgatcgcga cccggatcca ttcctcagcg gggccggggg cgtgccggcc
     5101 cgacccgatc gtcagcgatg cgaacagccc gatcgaggcc cagatggcga cccaggcgga
     5161 ggccgtcagc ggcggggtgc gacgcatcac gtgatccacg ccggtcaggt aggcggcgaa
     5221 catcaccgcg ctggccagcg cgagcgcgat ccccgccggg cggatggcaa ccgcgccgcc
     5281 cgccgccacc acgatcgcgg cgcccgcgat cgccgcggcc atcgcgccgg ccacgagccg
     5341 ccccggcgtt cccctcccga tcgccatcga tatgagcgcc acgaggacgg gataggtgaa
     5401 gaacagaagc gtgaccgcgg tcgcggtccc gtagcggatc gcggcgaaga acagcgcggc
     5461 ctcgaccgcg tagcccgcga gcccgagcag cgcgagccaa agccgctcgc ccgcggccgg
     5521 caacagcggc ttccgccgcc cggcttggat catgagcagc acggctccgg cgacggcgaa
     5581 tcggatcgcc agcatcgccg tgacgctcag cccgccgcgg agcagctggg tcccgacgac
     5641 gaccacgatg ccaaactgca gcgacgccag cgcgatcaag cccccgccga cgagcgattc
     5701 ccggtctcgc acgggggcca tcttgccagc gaagggggcg ttcggcccgg gaaaatcgcg
     5761 ggtagactcc ctgaacgtga ccccatcgga gcggaaagcg ctgcgcgaga gcttgctggc
     5821 ggagatgatc gccggcgata cgctcacgcc ccgcgagtcg gtcgtcggca acgccgagaa
     5881 gctcgccgcc ggcgaccggg acaagttctt cgggttcgac ttcggcgtgc tggacgcgca
     5941 atcggtgatc gacgatgtcg cggccgtgtg cggatgctcg aacgatctga gcgagcggac
     6001 gggccccggg gtcatcgacg tcgagcgaac cttggatgcg cttcacgcgg tcgccgaccg
     6061 tctccgggcc gcacgcagcc gcggcgagcg cgtgatgttc gctaccggtc atccgaccgg
     6121 gctcatgccc gtctacatgg agatcgcccg cacgctcgcg gcgaacggct gcaagctggt
     6181 cacgccgaga tcgggcgaga agctgcggca ggccttccac ggctcggtct ccagcgcctg
     6241 ggagatggcc cgccgtcgcg gcaaccgcgt gcgctacctc gacggggtcg gcgcgctgtc
     6301 ggacggcgcg cacctgtatc acacccacga atcctggccg atggagatgc tcctggacgg
     6361 catcgaccca ccggacctgg tgatcgccga tcacgggctg gccggggctg cgatcgtgcg
     6421 cggcgtcgag acactggcct tcgccgacgt caacgacccg gcgctgtcgg tggcgaagcg
     6481 tcgcgggatg accgaggtcg tcgtcccgct cgacgacaac cgcgatcccg agcgctacgc
     6541 ggccatgacc gcgttcctgg cggccggcgc cggcgcatga cgctcgctat caacccgggc
     6601 ccggaactgc cgataaacgg gaaaacggcc ggaacgcggg cttccgaaac gggaaaccgg
     6661 cgggcgggac ggaaggtgca ttggcgcgag ggtgcccgga tcacggacaa tagcgccgta
     6721 gccggttcct tccctgaacc aagccggtct ataatgggtc tactcagcgg aaggagaacg
     6781 gttggcgagt ccagtctcgc ccagcggccc ccgtttcctg actgcgttgg aggtcgctga
     6841 gcgcatgcgt gtttcgaaga tgaccgtgta ccggctcatc cggagcggca agcttcccgc
     6901 cgtccgggtg gggaaatcgt tccgtgtccg ggaagaggac gtcgacaagt atctcgactc
     6961 gagctacgtg cgagccgtct gatctcgtgg accacctcac ggaatcctgc ctgcgctgcc
     7021 gcgtgccttc cttcccccga gcaggtatac gaagcagcgc gtagtccgag atcggcgggg
     7081 agatcgtcct ggacgcatac gaaagcgtca tcgcccggat cgtgggcgcc ggcggcatgg
     7141 ccgtggcgct cggcggaatg gattccggca agaccacctt ctgcaaaatg gcggccgagg
     7201 tcgccgtgcg cgtcggccgc aaagtcgcgt acctggacac cgacgtcggc caaaccacca
     7261 tcggcccgcc ggccaccatc ggtatgaagt acctcgagca cgaggacgat ctgaagccgg
     7321 aagcgctggc ccacccggat gcgatgtatt tcgtcggctc cgtgaccccg caggggcatc
     7381 tgctgcccat cgtggtgggg gcatcgaaga tggccgagca ggcgcgggcc catggcgcgg
     7441 acatcatctt ggtggacacc accggcctga tcggtggcac cctcgggcag atcctgaagt
     7501 accacaagat cgaggtgctc cgtcccgaca tcgtcgtcgg attccagcgc ggtgaagaac
     7561 tcgagccgat cctgggcgcg atccggcgcg catttccgct caccgtcgag acgctcccgg
     7621 tgccggatga ggtgaagccg accaacgccg acgatcgcat cacgcaccgc caggagcgcc
     7681 ttcgcgccgc cttcgagccc ggcgtgcatc actggaaggt cagaatggga atcttcatcc
     7741 cgaacgtgcc gcccgatctc gacccggcgc tgctcgacgg gatgctcgtg ggactcgagg
     7801 acggcaccgg tcagtgcatg gggctcggga tcctcgagca tcgcgacgga tcgctgctgc
     7861 tccgctccac cgcggacgag ggggccaaag ctcttcgaat cggctcgacg cgcgtgtcgc
     7921 cggaatacat gacgagccgc gtcgacctcc gcgacctgtt cgtc
//
LOCUS       DATLFO010000214        15990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_86559, whole genome shotgun sequence.
ACCESSION   DATLFO010000214 DATLFO010000000
VERSION     DATLFO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86559"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VM841_08700"
     CDS             <1..598
                     /locus_tag="VM841_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVL90298.1"
                     /translation="FTLVCGAGTAEVTLPVPGEHMVWNALAAAAASLQLGATPAVIAA
                     ALASVETSPHRMRVVDAPGGWRVVDDAYNASPQSMSAALKTLARLGRGRRTWAVLGPM
                     AELGDLSAEEHDRAGRLAVRLGIARLVTVGREAQALHEAARLEGMNPDEAVLVDDAEA
                     AAAHVRAEIEPGDVVLVKASRVAGLERVVDALTGGGAA"
     gene            595..1638
                     /gene="mraY"
                     /locus_tag="VM841_08705"
     CDS             595..1638
                     /gene="mraY"
                     /locus_tag="VM841_08705"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HVL90299.1"
                     /translation="MRSVLYAAMVSLFVSLGGTPFLIRIFRLRRFGQLLREDWSDEGN
                     YHEQKRGTPTAGGIIIIVAIVAGYLAAHVPRAVPIKATGLAVLGTIIAMGAVGFTDDY
                     IKIRHQRSLGLNKTAKVLAQLAVAIGLAVVVIHFTGQSRFVTHLSFVRRTRLDLGEYL
                     FIAWVFVVLSATTNGVNLTDGLDGLAAGASAIVFTAYVFIAFWQFRHPIYEIHDALEL
                     AIVAAAGLGACAGFLWWNAAPARIFMGDTGSLALGGGIAALAIFTSTHLLLVVIGGLF
                     VLETTSVIMQVVAFRGFKRRVFKMAPVHHHFELGGWPEFTVIVRFWIIAGLSAALGIG
                     LFYADFLARGGLR"
     gene            1665..3014
                     /gene="murD"
                     /locus_tag="VM841_08710"
     CDS             1665..3014
                     /gene="murD"
                     /locus_tag="VM841_08710"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVL90300.1"
                     /translation="MLPGSGDRVLVGGLGISGLAAAAALIELGATVIVTDDGAGAEVP
                     GAARAVFGGMDPALIEDTDLLVVAPGVPPRAPLTAAARDAGVPVWSEIELGYRMTDSP
                     VIGVTGTNGKTTTTEMIAAALAGDGAIAAGNIGTTLTEVALQGAGVIVCELSSFQLEH
                     VVSFRPRIAVLLNIAEDHIDWHGSFDAYAAAKARIFANQTAGDTAIVHDDPACLPLAA
                     GPMRVVPFAATRLPEGGAGVEDGIVVASERLRFPVSSLQVEGTGMIADAVAAAAAADA
                     FGADPAAVARALAAFLPARHRTAIVATIGGVVYVNDSKATNPHATLAALEGRSGVVLI
                     AGGRNKGMDLSVLARASGLAGVVAIGESAGEVEAAFADSSIPVARAASMREAVGRATA
                     MAPAGGTVILSPACASFDWYGGYAERGDDFERIVLATAESGATGVTIGESEDAKGDA"
     gene            3011..4216
                     /gene="ftsW"
                     /locus_tag="VM841_08715"
     CDS             3011..4216
                     /gene="ftsW"
                     /locus_tag="VM841_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HVL90301.1"
                     /translation="MTVVQLARTRLGLRRKSRARARAKDDIVVLNSSGWMVAGISATL
                     LVFGLVMILSSSSVSAAENYGSPFYFVKRQLLWATVGLIAAVAVARSDYRRWRPLSWL
                     LLGVSLIGLVAVLLPGVGIRVSGSSRWLGFGWVRIQPSEFAKLALLLVAADVVARKQH
                     RLATMMEIFKPLGVITLLIIALVMKQPDLGTTLVIGLIVFVILFVGSVPMRLIAGMAA
                     AGVGGTLALAVSAPYRKERLMNFLDPFADALGSGYQAVQGLIAVGSGGMFGVGLGESR
                     QKWLYVPNAHTDFIFAIIGEELGLLGTLAVLALFLGLAYAGIRIARRAPDTFGRLVAA
                     GITVWIVGQAVINMGAVTGLLPITGVPLPLMSFGGSALVFTMAAIGMLVAVARREQWP
                     PPKEGDSKA"
     gene            4256..5359
                     /gene="murG"
                     /locus_tag="VM841_08720"
     CDS             4256..5359
                     /gene="murG"
                     /locus_tag="VM841_08720"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HVL90302.1"
                     /translation="MPTSIVIAGGGTGGHVYPGLALAEALRARRPDAAISFIGTERGL
                     EMRAVPAAGFELDTIAVLPWAKTLGARRYLAPATALRATVQAMRILRRRRAQVVVSMG
                     GYASLPAALAARLNGVPIVVHEQNAVPGRANRITARLTRRVAATFAESAAAFPRPSQV
                     RVTGNPIRESLARLDRDALRPEAFLHFGLDPGKTTVVVTGGSQGAKRLSEAARSLAAS
                     WAGDHAMQILLIAGRGNASDAAPGPIREIEFTDRMDLVYAAADLVVCRSGAGIMEVAA
                     AGVPAIMVPYPYARDDHQRANAAALERARAGIIIDETEASPERLAALIREIAGDPARR
                     GAMAAAARAYARPDAAAALAAMVLDAAEKGPSR"
     gene            5356..6765
                     /gene="murC"
                     /locus_tag="VM841_08725"
     CDS             5356..6765
                     /gene="murC"
                     /locus_tag="VM841_08725"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HVL90303.1"
                     /translation="MSEHARRIDDLSTVHLVGVGGAGMSALARVLVARGATVSGSDIK
                     ESRGLAALRALGVRAEVGHRAENLEGAGALVVSSAIPPHNPELQAARAAGIPVLQRAQ
                     VLAMLMRERRGVAVAGTHGKTTTTSMIAMAMRHAGLDPTFLIGGDLNETGTNAHHGTG
                     EWLVAEADESDGSFLWLAPDVAVVTNIEADHLDYYRDEDEIRQAFLSFVANVRPGGTV
                     VLCDDDPGSAGIADRIPVTVVRYGIGEGDGWRARVIERGPGGIRAAIARDGEHLGEAS
                     LSVAGDHNLRNALAAFAVADLAGVPFETAAEELAVFGGVARRFEFRGLANDITIVDDY
                     AHHPTEIAATLASARERGFDRIVAIFQPHRYSRTEAMGAALGAALAGADLAVVTDVYG
                     AGEQPVPGVTGKRVVDGLLDAVPRARVAYLPLGSDLAGFAARIGRPGDLILTLGAGDI
                     TMLADEILDRLRDRSPAVS"
     gene            6762..7700
                     /gene="murB"
                     /locus_tag="VM841_08730"
     CDS             6762..7700
                     /gene="murB"
                     /locus_tag="VM841_08730"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HVL90304.1"
                     /translation="MTRAETAERAAALIRARAHGRCETFMPLGSLTSYQLGGPADVYI
                     EAESEEDLAALADALDETGLPLLVIGRGSNMLVSDEGFPGIAVRLGGGFRWARVEGTA
                     VAAGGAMPVPALASLAAGASLAGLEFAAAIPASFGGAIAMNAGAHHRSISEVLIEATV
                     FDLSARSSRTIDAADLGFVYRASALPAGSIVTAGLAALGPGDPDEIAAGMREAREWRR
                     ATQPVGLPNAGSVFKNPPEEAAGSLVERICGKGTTHGGARISEVHANFIVAEPGATAA
                     DVHTLIRRIQRQVLENAGVRLETEVKLIGRFEESGE"
     gene            7781..8446
                     /locus_tag="VM841_08735"
     CDS             7781..8446
                     /locus_tag="VM841_08735"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HVL90305.1"
                     /translation="MVLTVTVLGGLVGGGVALTRSSLFALDGIEIAGVKMLSRAEVLE
                     ASGLRIGQNVLSVDAGRVESRVEDLPIVANASVERLYPSKVRIRVRERTPAAAAVVGG
                     ATWLIEASGRLIAPVAVPPAGMPRVTVRAEPGSPALEAALRLWSSLPDWARDKTTDLE
                     APEPEAIAARIGGTRIIFGDAESVLPKMQAVVAIFERAKTDGKRVKQIDVRAPHRPAA
                     VFA"
     gene            8936..9883
                     /gene="ftsZ"
                     /locus_tag="VM841_08740"
                     /pseudo
     CDS             8936..9883
                     /gene="ftsZ"
                     /locus_tag="VM841_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            10137..10820
                     /locus_tag="VM841_08745"
     CDS             10137..10820
                     /locus_tag="VM841_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HVL90306.1"
                     /translation="MFTTRHGGHGERGSLNLSLVAGDEPGRVLKNRARALTAVGASGG
                     DWTGGRQVHGTTIGRVTAAECGRGATDVATVLPDTDALWTDEPGAALVVLVADCVPIL
                     MADPRARRIAVVHAGWRGLVAGIVAKAAAVFDDPSRVIALAGPSIGPCCYEVGDEVAG
                     PATGRFGPGIMNGRHLDLWAGTAAAFRDAGIRDATLALACTRCESHRFFSHRAGDAAR
                     QGVIARLEP"
     gene            10821..11501
                     /locus_tag="VM841_08750"
     CDS             10821..11501
                     /locus_tag="VM841_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HVL90307.1"
                     /translation="MPPLPPPGLGAPAVLERIAAAAARAGRDPSAVTLVAAAKTVEPD
                     RIVAAGVADVGENRVQELAAKQEALAEYAIRWHFIGRLQRNKVKAVAGRVALVHSVDS
                     VELAHEIGRRAAEAGAVQPILIQVNVSGEASKGGVPVAEAPAVVDAVRAVEGIEVRGL
                     MTIPAPGDEAASRAAFRTLEAAAAALGLSELSMGMSGDFEVAVEEGATIVRVGTAIYG
                     PRPPAGGG"
     gene            11598..12074
                     /gene="sepF"
                     /locus_tag="VM841_08755"
     CDS             11598..12074
                     /gene="sepF"
                     /locus_tag="VM841_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HVL90308.1"
                     /translation="MAGLWKKTLVYLGLMEEDEELEEITPQAPERREEPTRMVRTLGR
                     DEQPSNHPAGRGVVRQLTTSASPRQVHVVEPRVYDDAQEFGDRFKAGVPVIINLRSTE
                     PRHAPKILHFASGLVYGLNGRMQKVADSVFLITPVNVEVSADEKRRLAEHGFFGEA"
     gene            12087..12344
                     /locus_tag="VM841_08760"
     CDS             12087..12344
                     /locus_tag="VM841_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVL90309.1"
                     /translation="MAIVCLLLRIYTFVIFARLILSWFPQIPESIRPIATALYAVTEP
                     VLRLARPLIPPVRIGMGAMDLSPILVFVVLGIISQAVCAAA"
     gene            12379..13362
                     /locus_tag="VM841_08765"
     CDS             12379..13362
                     /locus_tag="VM841_08765"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HVL90310.1"
                     /translation="MTITPHDIQHKQFREAFRGYNEEDVDTFLDEVAEEYGRVYAENQ
                     RLRIQLASVQQEAAYRGATGSTETPAAAPAGTASASPAVRAEERAEAREEMKRALVAT
                     QRAAEAALEEAKRRAAEIVARAEQRAKEIDEMTARRAKDVDSGAAGALTTIQARVEEL
                     RRQEIELRQRLRRMLAEHVRMLQQLEGEAVGATALTSAPSSAAVNGPADTPSPAAHMP
                     VAGDAAAASSSPDASVKRPTRWDSPRAKPVPDGPRPAGIGLRRAAGAEPPAPPPRPMG
                     VRPEGPATSTPSVAEPDTRAAVVARMTRELGDAANPPTEPAPADAREFWRR"
     gene            13482..14063
                     /locus_tag="VM841_08770"
     CDS             13482..14063
                     /locus_tag="VM841_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90311.1"
                     /translation="MIAASAVLVVVAFVTLIIGIFQTGLELIWVSIASSVLAAIFLLL
                     GVVKGRPPRVATAGAPYGEPAWGEAQSGAPTITEAPPAATEAPPAIILPDEEEDLVAD
                     EAEEEVLPPRRTTAAAGSASRTPAKKTAAKKTAAKKTAARTPAAQQQVVVIPDRDKFH
                     KTTCRYAKGAGTMKLTKSEARREGYKACGVCKP"
     gene            complement(14095..15306)
                     /locus_tag="VM841_08775"
     CDS             complement(14095..15306)
                     /locus_tag="VM841_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL90312.1"
                     /translation="MIAVESSRASDARRHLSATIDALQSAVSDSPELAIDALRAADRL
                     IASAVAAIARGVIDSAAGLPTPLVLQMDARAVGYEARAMTRTASVLSSMPRTLAAFRC
                     GLLSWSQTRGIVAAIKPCRVEDRIEIDRLIGSQVPSLIDADPDRIVEIVDDEVARRRR
                     DLTERREERAVERSFIAFQPRLGGRTMFYGEGDTASVATLVEALDRAAGAPANQEDDD
                     APTRAQQRYDALIALAGGSAAPSARPLVLATVDVNDLTAGETAARVLWNLAGRAPRLS
                     PIGTETMLCDSAVLPVVFDNGQPIAVGNTTQPIPRRIRRAVTTRDQGCRFPGCDRPVA
                     WTDAHHIRPRSHGGASTPANLISLCRRCHRRIHKRGWRIELEPDGVALFTHRARRYRS
                     DPATGPPRAAP"
     gene            complement(15495..>15990)
                     /locus_tag="VM841_08780"
     CDS             complement(15495..>15990)
                     /locus_tag="VM841_08780"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HVL90313.1"
                     /translation="RVVSLGLQAREQAKAKVRQPLARVWVEGADVPASLHVMVLEELN
                     VKSIEHGSPGDAQVASASEGGVTAHLDTEITSELRAEGNARELVRAVQNLRKNSGLAV
                     ADRISLTLDVSEDLWTALEPHREWIAAEVLATSFERGSSGSPLGKADVKLDGNKIPFA
                     IERV"
BASE COUNT         2313 a         5672 c         5629 g         2376 t
ORIGIN      
        1 cttcacgctg gtgtgcggtg ccggcacggc cgaggtgacg ctaccggttc cgggcgagca
       61 catggtgtgg aatgcgctcg cggccgcggc ggcctcgttg cagctcggcg cgacgccggc
      121 ggtgatcgcc gcggcgctcg cctccgtgga gacctcgccg caccggatgc gcgtcgtgga
      181 cgcgcccggc ggctggcgcg tcgtcgacga tgcctataac gcctcgccgc agtcgatgag
      241 cgcggcgctc aagacgctcg cgcgcctcgg ccgtggccgc cggacctggg ccgtgctcgg
      301 accgatggcc gagctcggcg acctgtcggc cgaggagcac gaccgcgccg gacggctcgc
      361 cgttcggctc ggcatcgcgc gactggtcac cgtcggccgc gaagcgcagg cgctgcacga
      421 ggccgcgcgg ctcgaaggca tgaatcccga cgaggccgtt ctggtcgacg acgcggaggc
      481 cgccgccgcg cacgtgcggg ccgaaatcga gcccggcgac gtggtgctgg tgaaagcatc
      541 ccgcgtggcg ggcctggaac gtgtcgtcga tgccctgacc gggggaggag ccgcatgagg
      601 tcggtcctgt acgcggcgat ggtcagcttg ttcgtctcgc tcggcggcac gccgttcctg
      661 atccgcatct tccgcctgcg ccggttcggg cagctgctgc gcgaggactg gagcgacgaa
      721 ggcaactatc acgagcagaa gcgcggcacg cccaccgccg gcgggatcat catcatcgtg
      781 gcgatcgtcg ccggctacct cgcggcccac gtgccgcgcg cggttccgat caaagccacc
      841 ggccttgcgg tcttgggcac gatcatcgcc atgggagcgg tcggcttcac cgacgactac
      901 atcaagatcc gccatcagcg aagcctcggg ctcaacaaga cggccaaggt gctcgcgcag
      961 ctcgcggtcg cgatcgggct cgcggtcgtg gtgatccact tcaccggaca gtcccggttc
     1021 gtgacgcatc tttcgttcgt gcgccgcacc cggctcgacc tgggggagta cctgttcatc
     1081 gcttgggtct tcgtcgtgct gtcggcgacc accaacggcg tcaacctcac cgacggcctc
     1141 gacgggctcg ccgccggcgc gagcgcgatc gtgttcaccg cctacgtctt catcgcgttc
     1201 tggcagttcc gtcatcccat ctacgagatc cacgacgcgc tcgagcttgc gatcgtcgcc
     1261 gccgccggcc tgggcgcatg cgcggggttc ttatggtgga acgccgcgcc cgcgcgaata
     1321 ttcatggggg acaccggctc gctcgccctc gggggcggca tcgcggctct ggccatcttc
     1381 acgtccacgc atctgctgct ggtggtcatc ggcggcttgt tcgtcctcga gaccacctcg
     1441 gtgatcatgc aggtggtcgc gttccgcggc ttcaagcggc gcgtgttcaa gatggcgccc
     1501 gtccatcatc acttcgagct cggtggctgg cccgagttca cggtgatcgt ccgcttctgg
     1561 atcatcgccg ggctgtccgc cgcgctcggc atcggcctgt tctacgccga cttcctcgcg
     1621 cgcgggggcc tgcgctgagc cggttcggcg accctgcgac gcgcgtgctt cccggcagcg
     1681 gcgaccgggt cctggtcggc gggctcggca tcagcggcct cgccgcggcc gccgcgttga
     1741 tcgagctcgg cgccaccgtc atcgtcaccg acgacggcgc gggcgccgag gtgcccggtg
     1801 ccgcccgcgc ggtcttcggc ggcatggacc cggcgctgat cgaggacacc gacctgctgg
     1861 tcgtcgcgcc cggcgtgccg ccgcgcgcgc cgctgaccgc cgcggcccgc gacgcgggcg
     1921 tgccggtctg gagcgagatc gagctcggct atcggatgac ggattcgccg gtcatcggcg
     1981 tgaccggcac caacggaaag accaccacga ccgagatgat cgccgcggcg ctcgccggcg
     2041 acggcgcgat cgcggccggc aacatcggca ccaccctcac cgaggtcgcc ttgcaaggcg
     2101 cgggcgtgat cgtatgcgag ctgtcctcgt tccagctcga gcacgtcgta tcgttccggc
     2161 cgcggatcgc ggtactgctc aacatcgccg aggaccatat cgactggcac ggcagcttcg
     2221 acgcctacgc cgcggccaag gcgaggatct tcgccaacca gaccgccggg gacaccgcga
     2281 tcgtgcacga cgacccggcc tgcttgccgc tggcggcggg cccgatgcgc gtggtcccgt
     2341 tcgcggcgac gcggctgccc gagggcggcg ccggcgtcga ggacgggatc gtggtcgcct
     2401 cggagcggct ccggttcccc gtgtcctcgc tgcaggtcga aggcaccggc atgatcgccg
     2461 acgccgtcgc cgccgccgcc gcggccgacg cgttcggcgc cgatccggcg gcggtcgcgc
     2521 gggcgctggc ggcgttcctg cccgcgcgtc accgcaccgc gatcgttgcg accatcggcg
     2581 gcgtggtcta cgtcaacgac tccaaggcca ccaacccgca cgcgacgctc gcggcgctgg
     2641 aagggcgaag cggcgtcgtg ctgatcgccg gcggacggaa caaaggcatg gatctgtcgg
     2701 tcctcgcccg ggcgagcggg ctcgccggcg tggtcgcgat cggggagtcg gcgggtgagg
     2761 tcgaggccgc gttcgctgac tcttcgatcc cggtcgcgcg ggccgcgtcc atgcgcgagg
     2821 cggtcgggcg cgcaaccgcg atggcgcccg cgggcggcac cgtcatcctc tcgcccgcct
     2881 gcgcgtcctt cgattggtac ggcggctacg ccgagcgcgg cgacgatttc gaacgcatcg
     2941 ttcttgccac cgcggaatcg ggcgcaaccg gggtgacaat aggggagtcc gaagacgcga
     3001 aaggagacgc gtgaccgtcg ttcaactggc ccggacccgg ctcggcctgc gtcggaaaag
     3061 cagggctcgt gcgcgcgcca aggacgacat cgtcgtgctc aatagctccg gctggatggt
     3121 cgcgggcatc tcggccacgc tgctggtgtt cggtctcgtc atgatcctgt cgtcgtcgtc
     3181 ggtctcggcc gccgagaact acggatcccc gttctacttc gtgaagcgtc agctgttgtg
     3241 ggccaccgtc ggtctgatcg ctgccgtcgc cgtcgcccgc agcgactacc gccgctggcg
     3301 gccgctgtcg tggctgctgc tgggcgtgtc gctcatcggt ctggtcgcgg tgctcctgcc
     3361 cggcgtcggc atccgcgtct cgggctcgtc gcgctggctc ggcttcggct gggtgcgcat
     3421 ccagccgtcg gagttcgcca agctcgcgct gctgctggtc gcggccgacg tcgtggcgcg
     3481 caagcagcat cgtctggcga cgatgatgga gatcttcaag ccgctcggtg tgatcacgct
     3541 gctgatcatc gcgctggtga tgaagcagcc ggatctgggc acgacgctgg tgatcgggct
     3601 gatcgtgttc gtgatcctgt tcgtcggcag cgtcccgatg cggctgattg ccggcatggc
     3661 cgccgcgggc gtcggcggaa cgctcgcgct cgcggtctcg gcgccctacc gcaaggaacg
     3721 gctgatgaac ttcctcgacc cgttcgccga cgcgctcggg tccggctatc aggccgtgca
     3781 agggctgatc gcggtcggct ccggcggcat gttcggcgtc ggcctcggcg agagccgcca
     3841 gaaatggctc tacgttccca acgcacacac cgacttcatc ttcgcgatca tcggtgaaga
     3901 gctcgggctg ctcggcacgc tcgcggtcct ggcgctgttc ctgggcctgg cctacgcggg
     3961 catccgcatc gcgcgccgcg cccccgacac cttcggccgc ctcgtcgcgg ccggcatcac
     4021 ggtttggatc gtcgggcaag cggtaatcaa catgggcgcc gtgaccggct tgctgccgat
     4081 caccggcgtg ccgttgcctc tgatgtcgtt cggcgggtcg gcgctggtgt tcacgatggc
     4141 cgcgatcggc atgctggtcg ccgtcgcgcg tcgcgagcag tggcccccgc ccaaggaagg
     4201 agactcgaag gcgtagcgcg cgtccccgcg cgggacgagc cctgcgccgg cgcacatgcc
     4261 aacgtccatc gtgatcgccg gcggcggaac cggcggacac gtctatcccg gcctggctct
     4321 cgccgaggcc ctgcgcgccc gtcgcccgga cgccgcaatc tccttcatcg gcaccgagcg
     4381 cggcctcgag atgcgggcgg tcccggcggc cggcttcgag ctcgacacga tcgcggtgct
     4441 gccgtgggcc aagacgctcg gcgcacgacg ttacctcgcg cccgcgaccg cgctgcgcgc
     4501 gaccgtgcag gcgatgcgca tccttcgccg ccgccgcgcg caggtggtgg tgagcatggg
     4561 cggatacgcg agcctgcccg cggcgctcgc cgcgcgtttg aacggcgtgc cgatcgtggt
     4621 ccacgagcag aacgccgtgc cgggacgcgc gaaccggatc accgcgcgcc tgacgcgccg
     4681 tgtcgcggcg accttcgcag agtccgccgc cgcatttccc cgtccctcgc aggtacgtgt
     4741 caccggaaat ccgattcgcg aatccctcgc acggctcgat cgcgacgcgc tccggccgga
     4801 agcgttcctg cacttcggcc tcgaccccgg caagaccacc gtcgtggtca ccggcggcag
     4861 ccagggcgcg aagcggctca gcgaagccgc gcgatccctg gcggcctcct gggcgggcga
     4921 ccacgcgatg cagatcctgc tcatcgcggg ccgcggcaac gcgagcgacg ccgcgcccgg
     4981 accgatccgt gagatcgagt tcaccgaccg gatggatctt gtctacgcgg ccgcggatct
     5041 ggtcgtgtgc cgctcgggtg ccgggatcat ggaggtggcc gccgccggcg tcccggccat
     5101 catggtgccc tatccctacg cgcgcgacga ccaccagcgc gccaacgctg cggcgctcga
     5161 gcgcgcccgc gcgggcatca tcatcgacga gaccgaggcc agtcccgagc gcctggccgc
     5221 cctgatccgc gagatcgccg gggatcccgc gcgccgcggc gcgatggcgg cggccgcacg
     5281 cgcgtatgct cggcccgacg ccgccgccgc gctggcggcg atggtactgg acgcggcgga
     5341 gaaaggaccg agcaggtgag cgagcacgcc cggcgcatcg acgacctttc caccgtgcac
     5401 ctggtcgggg tgggcggggc cggcatgagc gcgctcgcac gcgtgctggt cgcccgcggc
     5461 gccaccgtca gcggatccga catcaaggag tcccgcggcc tcgccgcgct gcgggcgctc
     5521 ggcgtgcgcg ccgaggtcgg gcaccgcgcc gagaacctcg aaggagcggg cgcgctcgtc
     5581 gtatccagtg cgatcccgcc gcacaacccc gagctgcagg ccgcgcgcgc cgccggcatc
     5641 ccggtgctac agcgcgcgca ggtgctcgcc atgctgatgc gcgagcggcg cggcgtcgcc
     5701 gtcgccggca cgcacggaaa gaccaccacc acctcgatga tcgcgatggc gatgcgccac
     5761 gcgggcctcg atcccacgtt cctgatcggc ggcgacctca acgagaccgg aaccaacgcg
     5821 caccacggca ccggcgaatg gctcgtcgcc gaggccgacg agtccgacgg ctcgttcctg
     5881 tggctcgcgc ccgacgtggc cgtcgtcacc aacatcgagg ccgaccatct tgattactac
     5941 cgggacgagg acgagatccg ccaggcgttc ctgagcttcg tggccaacgt ccgtccgggc
     6001 ggcaccgtcg tgctctgcga cgacgacccg gggtcggccg gcatcgcgga ccggattccc
     6061 gtaaccgtgg tgcgctacgg catcggcgaa ggcgacggct ggcgggcccg ggtcatcgag
     6121 cgcgggcccg gcgggattcg cgccgccatc gcccgagacg gcgagcatct gggcgaggcg
     6181 agcctgtcgg tcgccggcga ccacaacctt cgcaacgcgc tcgccgcctt cgccgtcgcg
     6241 gacctggcgg gggtcccgtt cgagaccgcg gccgaggagc tcgccgtctt cggcggcgtg
     6301 gcccggcgat tcgagttccg cggcctcgcc aacgacatca ccatcgtgga cgactacgcc
     6361 caccatccca cggagatcgc cgcgacgctc gcctcggcgc gcgagcgcgg cttcgacagg
     6421 atcgtggcca tcttccagcc ccaccgctac tcgcgcaccg aggcgatggg cgcggcgctg
     6481 ggcgcggccc tggccggtgc ggacctggcc gtcgtgaccg acgtctacgg agccggcgag
     6541 cagccggtcc ccggcgtgac cggcaagcgg gtggtggacg gcctgctcga cgcggtacct
     6601 cgcgcgcggg tggcgtacct gcccctcggc agcgacctgg ccggcttcgc cgcccggatc
     6661 ggccgccccg gcgacctgat cctgacactc ggggcggggg acatcacgat gctcgccgac
     6721 gagatcctgg accggctgcg ggaccgcagc cccgccgtgt cgtgacccga gccgagaccg
     6781 ccgagcgggc ggccgccctg atccgcgccc gcgcccacgg ccgctgcgag accttcatgc
     6841 cgctgggctc gctgacctcc tatcagctcg gcggccccgc cgacgtctat atagaggccg
     6901 agagcgagga ggacctcgcc gcgctcgccg acgcgctcga cgagaccggg ctgccgcttc
     6961 tggtgatcgg ccgcggatcg aacatgctcg tctccgacga aggcttcccc gggatcgcgg
     7021 tccggctcgg cggcgggttc cgctgggcgc gcgttgaagg gaccgccgtg gccgcgggcg
     7081 gggccatgcc cgtgccggca ctcgcttcgc tggccgccgg ggcgagcctg gccggcctcg
     7141 agttcgccgc ggccatcccg gcaagcttcg gcggcgcgat cgcgatgaac gcgggcgcgc
     7201 accatcgaag catctccgag gtgctcatcg aggccacggt tttcgacctc agcgcacgct
     7261 cctcccgcac aatcgacgcc gccgatctcg gcttcgtcta ccgggcgagc gcgcttccgg
     7321 ccggctcgat cgtgaccgcc gggctcgccg cgctcggccc gggcgatccc gacgagatcg
     7381 ccgccggcat gcgcgaggcc cgcgaatggc gccgggcgac ccagcccgtc ggcctgccga
     7441 acgccggcag cgtcttcaag aacccgcccg aggaggccgc cggcagcctg gtcgagcgga
     7501 tctgcggcaa gggaaccacg cacggcggcg cacggatcag cgaggtccac gccaacttca
     7561 tcgtcgccga gcccggcgcc accgccgccg acgtccatac cttgatccgc cggatccagc
     7621 gccaggtgtt ggagaatgcg ggcgtgcgac tcgagaccga agtgaagctc atcggccggt
     7681 tcgaggaatc gggcgagtga aaaagcgcac gccccggacc gctcgggctc ccgccggcac
     7741 gcgccgcgcc cgccgggcca ggctcagaaa ggtccgccgg atggtgttga ccgtcaccgt
     7801 gctcggcggc ctcgtcggag gcggcgtcgc gctcacccgc tcgtcgctgt tcgccctcga
     7861 cggcatcgag atcgccggcg tcaagatgct cagccgcgcc gaggtgctgg aagcgagcgg
     7921 tctgcggatc ggccagaacg ttctgtcggt ggacgccggc cgggtcgagt cgcgcgtcga
     7981 ggatctgccc atcgtggcga acgcgagcgt cgagcgtctg tacccctcga aggtccggat
     8041 ccgggtccgg gaacgcaccc ccgccgcggc cgccgtcgtc gggggagcga cctggctcat
     8101 cgaggcctcc ggacggctga tcgcaccggt ggccgtgccg ccggccggca tgccgcgggt
     8161 caccgtccgc gccgagcccg gctcgcccgc cttggaggcg gccttgcggc tgtggagcag
     8221 cttgcccgat tgggcgcggg ataagacgac cgacctggaa gcccccgagc cggaggcgat
     8281 cgccgcgcgc atcggcggaa ctcgcatcat cttcggggac gccgaatcgg tcctgccgaa
     8341 gatgcaagcg gtcgtggcga tcttcgagcg agcgaagacc gacggcaagc gggtcaagca
     8401 gatcgacgtc cgcgcgcccc atcgccccgc tgccgtcttc gcatagcgcg gatcaaccgg
     8461 gccgcttcgt ccgttcgccg ctccatcggc gatgctcagt aggagccagc cccatccctc
     8521 gaaaacacgt gttattgccc gaaaaattgt tctgattccg gcttgactag aacatgaggg
     8581 ctcgtttact gtctcgaccg tcagatgagg tttacataac actcagccta aagttgagcg
     8641 ttaacaccgg atctgaccag cgcaaataac ggatctgcct tcacggcgtg ccttccccaa
     8701 cgcttgtggc tcaagtaacg ctcgagggga tcggaacctt caactaaggg ggtacccggt
     8761 ctgaaacggt gagccaccgg cgacacgcca gccgaatttt cggcgggtgg gccctaagcg
     8821 ccgcttcggc ttcccctgaa gcggacgccg ggttattgaa cggactcccg ggcatcgggg
     8881 gtccggccgt aggggagtag agagtggtcg tggccaccga aggaggcggt ccgtaatggc
     8941 aacaaccccg cagaactatc tcgcggtcat caaggtcgtg ggcatcggcg gcggcggcgt
     9001 aaacgccgtc aaccgcatga tcgaggaagg cctcaagggc gtcgagttca tcgccatcaa
     9061 cacggacgcg caggcgcttc tgatgtcgga tgcggacctc aagctcgaca tcgggcgtga
     9121 gatcacccgc ggcctcggag ccggctccga cccggagcag ggtcgccgcg ccgtcgaaga
     9181 cgcgcgcgac accatcgagg aggttctcaa gggagccgac atggtgttcg tcaccgccgg
     9241 cgaaggcggc ggcaccggca ccggcggcgc gccgatcgtg gccgaggtcg ccaagtcgct
     9301 cggcgccctg acgatcggtg tcgtcacccg tccgttcagc ttcgagcgca aggtccgcgg
     9361 cgagcaggcc gagcgcggca tccaagagct aaagaagcgg gtcgacacgc tcatcgtcat
     9421 cccgaacgac cgtttgctcg acgtcgccga cgagaacacc acgatgctgg acgccttcaa
     9481 gatggcggac caggtcctgt tccaaggcgt ccagggcatc accgacctga tcaccacgcc
     9541 gggcgtcatc aacctggact tcgcggacgt ccgcgcgatc ctgaccgatg ccggttcggc
     9601 ccttctgggt gtaggccacg cccgcgccga gaaccgcgcc gtcgaggccg ctcgcgcggc
     9661 gatctccagc ccgctgctgg agtcctcgat cgacggagcc cgcggcgtgc tgctgtcgat
     9721 caccggcggc agcgacctca cgctctatga gatcaacgag gccgcccgga tcatcagcga
     9781 ccacgcgcac ccggacgcca acgtcatctt cggtacggcc atcgacgact cgctgggcga
     9841 cgaggtccgc gtgaccgtca ttgccgccgg gttcgagcgg tgggcgactc gcgaggtcgg
     9901 cgccgacctg acgctcgacg aggacgagga ggacgagttc acgtccgagc cggccaagcc
     9961 gcgccggacg ctcgtgttcg actccgacga cgaattggaa gacatcccct ccttcttgag
    10021 gcggtaacga gaaggggaca ggcctgttcg cctggggagc atttccgggg cgggcgggag
    10081 cggagcccgg cgcgtatctc gggtcccccg ctctcgcccg ctccggcatt tccgcgatct
    10141 tcaccacacg ccacggcggc cacggcgagc gcggctcgct gaacctgtcg ctggtcgccg
    10201 gggacgagcc cggccgcgtc ctgaaaaacc gggcccgggc cctgaccgcg gtcggggcga
    10261 gcggcggcga ctggaccggc ggccgtcagg tgcacggcac cacgatcggc cgcgtcaccg
    10321 cagcggaatg cgggcgcggg gcgaccgacg tcgcgacggt cctgcccgac accgacgcgc
    10381 tctggaccga cgagcccggt gccgcgctgg tcgtcctggt cgccgactgc gtcccgatcc
    10441 tgatggccga cccgagggca cgtcgcatcg cggtggtgca cgcgggctgg cgcggtctcg
    10501 tcgcgggcat cgtcgccaag gccgccgccg tcttcgacga tccctcccgc gtgatcgcgc
    10561 tcgccggacc gtcgatcggg ccgtgctgct acgaagtcgg cgacgaggtt gccggcccgg
    10621 ccacggggcg gttcggtccc gggatcatga acgggcgcca cctcgacctg tgggccggaa
    10681 cggcggccgc gttccgcgat gcgggcattc gcgacgcgac cctcgcgctt gcgtgcaccc
    10741 gctgcgagtc gcatcgcttc ttctcgcacc gcgccggcga cgcggcccgg cagggcgtca
    10801 tcgcccggct ggagccgtag atgccgccgc tgccgccgcc cggactcggc gcgcccgccg
    10861 tgctcgagcg catcgccgcc gccgccgcgc gcgccgggcg cgatccgtcc gcggtgacgt
    10921 tggtcgccgc ggccaagacc gtcgagcccg accggatcgt ggccgccgga gtcgccgacg
    10981 tcggcgagaa ccgggtccag gagctggccg ccaagcagga ggcgctcgcc gagtatgcga
    11041 tccgctggca tttcatcggc cggttgcagc gcaacaaggt caaggcggtg gccggccggg
    11101 tcgcgctggt ccattcggtg gactcggtcg agctggcaca cgagatcggc cggcgggccg
    11161 ccgaggccgg cgcggtccag ccgatcctga tccaggtcaa cgtcagcggc gaagcgagca
    11221 aaggcggcgt gcccgtcgcc gaggcgccgg cagtggtcga tgcggtccgg gccgtcgagg
    11281 gcatcgaggt tcggggattg atgacgattc ccgcacccgg ggacgaggcc gcgagccggg
    11341 ccgcgttccg caccctggag gccgcggccg cggcgctcgg gctgagcgag ctctccatgg
    11401 gaatgagcgg cgacttcgag gtcgccgtcg aggagggagc cacgatcgtg cgggtcggaa
    11461 ccgcgatcta cggaccccgt ccgccggcag gcggcgggta gaatcggcat gggtcgggcg
    11521 cgcgcttcca tcttcgacgc cgatccgtgt ctcctggcat aacatgccga catcgcacag
    11581 gaggcggaga gaacacgatg gccggactgt ggaagaaaac actcgtctac ctcgggctca
    11641 tggaagagga cgaggagctc gaagagatca cgccgcaagc gccggagcgg cgcgaagagc
    11701 cgacccgcat ggttcggacg ctgggccgtg acgagcagcc gtccaaccat ccggccggac
    11761 gcggggtcgt tcgtcagctg acgacgtcgg cgtcgccgcg ccaggtccac gtggtcgagc
    11821 cccgcgtcta cgacgacgcg caggagttcg gcgaccggtt caaggccggc gtcccggtga
    11881 tcatcaacct gcgctcgacc gagccgcggc acgcgcccaa gatcctgcac ttcgcatcgg
    11941 gactggtcta cgggctcaac ggccggatgc agaaggtcgc cgactcggtc tttttgatca
    12001 cgccggtcaa cgtcgaggtc tccgccgatg agaagcggcg cctcgccgag cacggcttct
    12061 tcggggaagc gtaaggaccg ctcgtcgtgg cgatcgtctg tctgctgctg cgcatctaca
    12121 cgttcgtgat cttcgcgcgg ctgatcttgt cgtggtttcc gcagatcccc gagtccatcc
    12181 gcccgatcgc caccgcgctc tatgcggtga ccgagccggt tctgcgtctc gcgcgcccgc
    12241 tcatcccgcc ggtgaggatc ggcatgggcg ctatggatct ctcgcccatt ttggtcttcg
    12301 ttgtgctggg aatcatctcc caggcagtgt gcgcggcggc gtgactttcg gcgtcggcat
    12361 ggtagtttcg gtggcgcgat gaccatcacc ccgcacgaca ttcagcacaa gcagttccgc
    12421 gaggcgttcc gcggctacaa cgaagaggac gtagacacct tcctcgacga ggtggccgag
    12481 gagtacgggc gcgtctacgc cgagaaccag cgtctccgaa tccagcttgc ttccgtccag
    12541 caagaagccg cgtaccgcgg cgcgacgggc tcgaccgaga cgcccgccgc tgccccggcc
    12601 ggtaccgcgt ccgcgtcgcc ggcggtgcgc gccgaggagc gcgccgaggc tcgcgaggag
    12661 atgaagcgag cgctcgtggc gacgcagcgc gcggccgagg ccgcgctcga ggaggccaag
    12721 cggcgcgccg ccgagatcgt cgcgcgcgcc gagcagcgcg ccaaggagat cgacgagatg
    12781 accgcgcgac gcgccaagga tgtggactcc ggcgcggcgg gcgcgctgac caccatccag
    12841 gcgcgcgtcg aggagctgcg ccggcaggag atcgagcttc gtcagcggct gcgccgcatg
    12901 ctcgccgagc acgtgaggat gctccagcag ctcgaggggg aggccgtcgg cgccacggcg
    12961 ctcacgtctg ccccgtcatc cgctgccgtc aacggacccg cggatacgcc gtcgccagcg
    13021 gcgcacatgc cggtggccgg cgatgccgct gccgcatcgt cgtctccgga cgcatccgtc
    13081 aagcgtccca cgcgatggga tagcccgcgg gccaagcccg tgccggacgg accgaggccc
    13141 gcggggatcg gcttgcgccg cgccgccggg gccgagccgc ccgcgccgcc gccccggccg
    13201 atgggcgtgc gacccgaggg tccggccacc tcgaccccgt cggtcgccga gccggacacg
    13261 cgtgccgccg tcgtggcgcg aatgacccgc gagctcggcg atgccgccaa tcctcccacg
    13321 gagccggccc cggccgacgc ccgcgagttc tggcgccgct gacgcttcga ggttcgcatt
    13381 ccctcgcttt ggcccgatag gctggttcct ggcgtgcttt ggggctaagc gttagactcg
    13441 ggcgccgttc ggaccgacga acatacggaa ggagctaggt tttgatcgca gcgagcgccg
    13501 tcctggtcgt cgtcgctttc gtgacgctga tcatcggcat ctttcagacc ggcctcgagc
    13561 tgatctgggt gtcgatcgca tcgagcgtcc tcgccgcgat cttcctgctg ctcggcgtcg
    13621 tcaagggccg gccgccccgt gtcgcaaccg ccggagcgcc ctacggcgag cccgcatggg
    13681 gcgaggcgca aagcggagcg cccaccatca ccgaagcgcc gcccgccgcg accgaagcgc
    13741 cgcccgcgat cattctcccc gacgaggaag aggatctcgt ggccgacgag gccgaggaag
    13801 aggtcctgcc tccgcgccgc accaccgccg cagccgggtc cgcatcgcgt acgcccgcga
    13861 agaagaccgc cgcgaagaag accgcggcca agaagaccgc cgcccgcacg ccggccgcgc
    13921 agcagcaggt cgtggtcatt cccgaccgcg acaagttcca caagacgacc tgccgctacg
    13981 ccaagggagc cggcacgatg aagctcacca agagcgaagc ccgccgcgaa ggctacaaag
    14041 cctgcggcgt ctgcaagccg taagcagttt ccttcactgg cgtaagcggg cacgtcacgg
    14101 ggcggcgcga ggcggacccg tcgccggatc gctccggtag cggcgggctc ggtgcgtgaa
    14161 cagcgcgact ccatccggtt ctagctctat tcgccatcct cgtttgtgaa tgcggcgatg
    14221 gcagcgccgg cacaggctga tcagattcgc cggcgtggaa gcgcctccat gtgagcgcgg
    14281 ccggatgtga tgagcatccg tccatgcgac gggccggtcg cagccgggga accggcatcc
    14341 ctgatcgcgg gtcgtgacgg cacgccggat ccggcggggg atgggctgcg tcgtgttgcc
    14401 gacggcgatc ggctgtccgt tgtcgaacac gaccggaagc acggccgagt cgcagagcat
    14461 ggtttcggtt ccgatcgggg acagtcgcgg tgcgcggccc gccagattcc aaaggacgcg
    14521 cgcggcggtc tcgccggctg tcaggtcatt gacatcgacg gtcgccagca cgagcggccg
    14581 cgccgatggc gcggcagacc cgccggcgag cgcgatcaac gcgtcgtagc gctgctgcgc
    14641 gcgcgtcggc gcgtcgtcgt cttcttgatt cgccggagcg ccggcggcgc ggtccaaggc
    14701 ttcgacgagc gtcgcgaccg aagcggtgtc gccttcgccg tagaacatgg tgcggccgcc
    14761 gagccgcggc tggaacgcga tgaagcttcg ttcgacggcg cgttcctcgc gccgttcggt
    14821 caggtcgcgg cggcggcggg cgacctcatc gtcgacgatc tcgacgatac ggtccggatc
    14881 ggcatcgatc aacgaaggaa cctgggagcc tatgagccgg tcgatttcga tgcggtcttc
    14941 cacgcggcac ggcttgatcg cggccacgat gccgcgcgtc tgcgaccacg acagcagacc
    15001 gcagcgaaac gccgcgagcg tgcgcggcat cgacgaaagc actgacgcgg tgcgagtcat
    15061 cgcgcgggcc tcgtagccca cggcccgcgc gtccatttgc agcacgagcg gagtggggag
    15121 cccggcggcc gaatcgatca cgccgcgtgc gattgcggcg acggcagaag cgatcagacg
    15181 gtcggccgcg cgaagcgcgt cgatcgcaag ctccggcgag tccgaaactg cggactgcaa
    15241 tgcgtcgatg gtcgcagaca ggtgacgacg agcgtcggac gcgcgactgg attcgactgc
    15301 gatcatgcca ccaatctaga acaagggtat gacagcaacg atcggtctta ggcggagtgc
    15361 gatcgcctgc cgggaattcg ttactgggcg aggaattcgc gactgcccgg catgtgggcg
    15421 ctcggtcaag aagggtctga cggtttgtcg atcatgcggc cacgatttcg ctgcggcggc
    15481 ggctcacgcg tgagctacac gcgttcgatg gcgaagggga tcttgttgcc gtccagcttc
    15541 acgtcggctt tgccgagcgg cgagccggac gagccgcgct cgaacgaggt ggcgagcacc
    15601 tcggcggcga tccattcgcg gtgcggctcc aaagccgtcc acagatcctc ggacacgtcc
    15661 agcgtgagcg agatccggtc ggcgacggcg aggccgctgt tctttcgcag gttctgaact
    15721 gcccgcacga gctcgcgcgc gttgccttcc gcgcgcagct cggaggtaat ctcggtgtcc
    15781 aggtgcgcgg tcacgccgcc ttccgacgcg gacgcgacct gagcatcccc gggagagccg
    15841 tgctcgatcg acttcacgtt cagctcttcc agcaccatga cgtgcagcga cgccggcacg
    15901 tccgcgcctt cgacccacac gcgcgccagc ggctggcgca ccttcgcctt cgcctgctcg
    15961 cgcgcctgta accccagcga cacgacgcgg
//
LOCUS       DATLFO010000215         7658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_123147, whole genome shotgun sequence.
ACCESSION   DATLFO010000215 DATLFO010000000
VERSION     DATLFO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123147"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VM841_08785"
     CDS             <1..699
                     /locus_tag="VM841_08785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90314.1"
                     /translation="PFYDEMVAGLGVDDPATVPTLAEYAGAGRALELGVGTGRVAIPL
                     AGRGIEVHGIDVSEAMIAKLKEKPGGGRITCVIGDFEDVPVEGAFSLIYCVFNTFFAL
                     TSAEEQIDCFANVAAHLDPGGFFLIEAFVPDMTRFDGAQRVQIQSLDADRVRLGVSIH
                     DSVNQRIHGQHIEMTEKGIEMVPIELRYAWPSELDLMARLSGLRLRERWGGWRREPFT
                     VASTQHVSLYEKPR"
     gene            846..1787
                     /locus_tag="VM841_08790"
     CDS             846..1787
                     /locus_tag="VM841_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HVL90315.1"
                     /translation="MRAIRTMVAAAALAAALAGPGGATATSNPWLGGPVLNIAHQGGE
                     IEAPSNTLYAFKTAVAKGADVLELDVHATADGEIVVIHDATVNRTTNGAGRVDSMALA
                     QIKTLDAAHWFVPGIGTTHSRPAGEYVLRGMATGARDPLPGFDAADFGIPTLRAVLEA
                     FPDIRINIEIKASAPDTTPYEQALAALLDEYGRADDVIVVSFLDHAVELFKVWAPEVH
                     TATATAETAAFWAAARGPGAPNPRYVAMQVPITFNGIPVIDDAGEFVAKAHANGLAVH
                     VWTINDAATMCRLIAMGVDGIMTDRPTLLEELLVNGC"
     gene            1829..3646
                     /gene="selB"
                     /locus_tag="VM841_08795"
     CDS             1829..3646
                     /gene="selB"
                     /locus_tag="VM841_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HVL90316.1"
                     /translation="MFVLGTAGHVDHGKSTLITRLTGIDPDRLAEEKARGLTIDLGFA
                     WTTLPSGREIGIVDVPGHERFVHNMLAGAGQVDAVVFVVDANEGWRPQSEEHLAILDL
                     LGARGGVVALTKVDTAPDAAAAAESVRARIAGTVLNDAEVVPVSAVTGEGIDTLVTAI
                     EALLDRTPEPFDRGRPRLFVDRSFSIKGAGTVVTGTLTGGCLREGEEVEILPPGTRAR
                     IRSLQTHRTARTEAVPTSRVALNLAGLDRHEIRRGDAVVAPGAWRVTTTIDAELHGVR
                     SLDHAVTGKGAFLLYAGTAETPARIRLLDAERIEAGTRAFARITTTEPVVAAPGDRIV
                     LRDTGRRQTVAGGTVLDAHPPHGRTGDDRVAQLNARAAAATEPAVLATLTVSERGVVA
                     RADRAWLTAADPGDAVGLPSYWVSRAWFDAAAKRLRDGLRRYHAHTPLEKGIPRDTLR
                     RDAGIGDAKLFDEMLDAMKDEIDQDAAIVRLAGHRVTLSPEQDAVRAKLIAEMDASGF
                     SPPPLSGLASRYGAALIKALTDAGALIKINTDIAYTAERFDAAKAAIADAVNAEGPLT
                     AGRIKEILQTSRKYAIPLLERLDAEGFTRRDGDVRIVRS"
     gene            complement(3606..3890)
                     /locus_tag="VM841_08800"
     CDS             complement(3606..3890)
                     /locus_tag="VM841_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90317.1"
                     /translation="MLMVTFWCSWCGATTRADSAAMRVSCRRCAVELWKLLGMRFGTD
                     MVPLDPGQQPPEPAAARKQFRFVDPAGNEVGRTEVDPLRSERSVRRRRGA"
     gene            complement(4020..4481)
                     /locus_tag="VM841_08805"
     CDS             complement(4020..4481)
                     /locus_tag="VM841_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVL90318.1"
                     /translation="MSAEERLSSLGLVLPEPPKAVAAYVPTAVSGATVYVSGQVPFRD
                     GALTRTGLVGRDVTLEDAVDDARVCAVNVLAQLRDAAGGSLDNVARIVKVTVFVASSE
                     GFHAQPTVANGASELFVEVFGDAGRHARAAVGVAALPMGATVEVEAIAELS"
     gene            complement(4478..4636)
                     /locus_tag="VM841_08810"
     CDS             complement(4478..4636)
                     /locus_tag="VM841_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HVL90319.1"
                     /translation="MPQWEYASVPLIPHAIQEILNNWGDEGWELVQVYNPTDVPGTVA
                     LLKRPKQS"
     gene            complement(4781..5062)
                     /locus_tag="VM841_08815"
     CDS             complement(4781..5062)
                     /locus_tag="VM841_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVL90320.1"
                     /translation="MRTDTVRNLYETDDGWQSRAACRGRDATLFFSPATGELKEEKLA
                     REARAKTICRECPVRTQCLEFALDTREPYGIWGGLNELERRRLMAKRAG"
     gene            complement(5221..6540)
                     /locus_tag="VM841_08820"
     CDS             complement(5221..6540)
                     /locus_tag="VM841_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HVL90321.1"
                     /translation="MRFRAAALAAFLLSLPLLFPALARAETGAAVPHAQKDDAPVVDI
                     IEISGLIDPPMAEYIADEIREANKEKAELLVISISSDGGLNTRTESLLRLIEASRVPV
                     AVYVGPQRAVAGGSAAVLTAAAHIAAIGPSARLGPAHPTNLAVKADSDRGRALRTATL
                     EQLNELAAARGRAPAIYLDEAIPASVALDRRQVDYTVVSVAELLQRANGREVTTAAGS
                     RTLNLDKDAVDIRFHKPGPWPRVLHTLANASLVYILLVAGALLIAFELFQPGFGVAGV
                     TGALLLVGGVYGLTVLPSSLWAVIALTGGTILMTLDVKLDGLGVPTAVGAVAFSAGSF
                     GLFPGPAAPLRIAWWLALLGVVSALVFFVPVMTLVRRARQPVRKNADASLVGQAGQVR
                     SILNPEGYVWVADALWRARADEGERIRVGEDVLVTGLEGTLLRVRRA"
     gene            6617..7612
                     /locus_tag="VM841_08825"
     CDS             6617..7612
                     /locus_tag="VM841_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HVL90322.1"
                     /translation="MTAQAAKLATASLTESRLIVVSGKGGVGKTTVAAAIATAAAAAG
                     KKVLLAEVEQRDGLAPLFGRPSMSYAEVSLAQNITGIAVVPDEALTEYLQLFYGIGRI
                     ARPLLTGKAVEFATSTAPGLRDILLIGKIKEAENRRTGGMYTFDLIVLDAPPTGRLPR
                     FLDAPRAVVELVQSGAIRQQAQGVLDMLTDPRRTRVVLVTLPEEMPVRETIEGAGTMR
                     EMGIALGPVIVNGLSPQPFKTGQQRALAKDGAAALLAEAGAAGVDLSEEAALGVASVA
                     NAHARRAANQRRALKELTDELDLSQIRLPALFTPQIGPAERRRLADELVAQGAIA"
BASE COUNT         1259 a         2690 c         2621 g         1088 t
ORIGIN      
        1 cccttctacg acgagatggt cgcgggcctg ggcgtggacg atcccgcgac ggtaccgacg
       61 ctggccgagt acgccggcgc cggccgcgcg ctggagctgg gggtcgggac cggccgcgtg
      121 gcgattccgc tcgcgggccg cggcatcgag gtgcacggca tcgacgtctc ggaggcgatg
      181 atcgccaagc tgaaagaaaa gcccggcggc ggccgcatca cctgcgtgat cggcgacttc
      241 gaagatgtgc cggtggaggg cgccttctcg ctgatctact gcgtgttcaa caccttcttc
      301 gcgctcacgt cggccgagga gcagatcgac tgcttcgcca acgtcgccgc gcatctggat
      361 cccggcggct tcttcctgat cgaagcgttc gtgccggaca tgacgcgctt cgacggcgcg
      421 cagcgcgtcc agatccagtc attggatgcc gaccgcgtcc gcctgggcgt gtcgatccac
      481 gactcggtga accagcggat ccacggccag cacatcgaga tgaccgagaa gggcatcgag
      541 atggtcccga tcgagctgcg ctacgcgtgg ccgagcgagc tggacctgat ggctcgtctg
      601 tccggccttc ggctgcgcga gcgatggggc ggctggcgcc gcgagccctt caccgtcgcc
      661 agcacccagc acgtgtccct gtacgaaaag ccgcgctaga cccccgccgt cggcagccgc
      721 tcgccccgcg ccgctcacgc aagccggctc ccgtcgcacg ggcagcgaac cgctgccgca
      781 tccaggcagg aggagtcccc gtcatggcga atctttggga acacacatcc gggaggaacg
      841 agaagatgcg agcgattcga acgatggtgg cggctgcggc gctggcggcc gcgctggcag
      901 gaccgggcgg cgcgacggcg acgtcgaacc cctggctcgg cgggcccgtg ctgaacatcg
      961 cgcaccaggg cggcgagatc gaagccccct ccaacacgct gtatgcgttc aagaccgcgg
     1021 tggccaaggg cgcggacgtg ctcgagctgg atgtgcacgc gaccgccgac ggcgagatcg
     1081 tggtcatcca cgacgcgacg gtcaaccgga ccacgaacgg cgcgggccgc gtggactcga
     1141 tggccctggc gcagatcaag acgctggatg ctgcgcactg gttcgtcccg ggcatcggca
     1201 caacgcactc gcgtccggcc ggcgagtacg tcctgcgggg catggcaacc ggcgcccgcg
     1261 atccgttgcc cggcttcgat gcggccgact tcggcatccc gaccctgcgc gcggtcctgg
     1321 aggcgttccc cgacattcgg atcaacatcg agatcaaggc cagcgcgccc gacacgactc
     1381 cctacgagca ggccctggcc gcgctgctcg acgagtacgg ccgcgcggat gacgtgatcg
     1441 tcgtatcgtt cctcgaccac gccgtcgagc tgttcaaggt gtgggcaccc gaggtgcaca
     1501 ccgcgacggc aaccgccgag accgccgcat tctgggcggc cgctcgcgga ccgggcgcgc
     1561 cgaacccgcg ctacgtggcg atgcaggtgc cgatcacgtt caacggcatc ccggtcatcg
     1621 acgacgccgg cgaattcgtc gccaaggcgc atgcgaacgg cctggccgtg cacgtatgga
     1681 cgatcaacga tgccgcaacg atgtgcaggc tgatcgcgat gggcgtggac ggaatcatga
     1741 ccgaccgtcc gacgctgctc gaggagttgc tcgtcaacgg gtgctgacga ccacgcacga
     1801 ccacggccct gcgcgatagc gtgaggccat gttcgttctc ggcaccgccg gccacgtcga
     1861 tcacggcaag tccacgctga tcacgcgcct gaccggcatc gatccggacc ggctcgccga
     1921 agagaaggcg cgcggcctga ccatcgatct gggctttgcg tggacgacac tgccctcggg
     1981 ccgcgagatc ggcatcgtgg acgtgcccgg acacgaacgc ttcgtgcaca acatgctcgc
     2041 cggcgccggc caagtagatg cggtcgtgtt cgtcgtggac gccaacgaag gctggcgccc
     2101 gcaatccgag gagcatctgg cgatcctgga tctgctcggc gcgcgcggcg gtgtcgtcgc
     2161 gctgaccaag gtcgacaccg cacccgacgc tgccgccgcg gccgagtccg ttcgcgcgcg
     2221 catcgccggc accgtgctaa acgatgccga ggtcgtcccg gtcagcgccg tcaccgggga
     2281 gggaatcgac acgctggtca ccgcgatcga ggccctgctg gaccgcacgc ccgaaccctt
     2341 cgaccgcggg cggccgcgac tgttcgtgga ccgctcgttt tcgataaaag gcgccggcac
     2401 ggtcgtcacc ggcacgctga cgggcggatg cctgcgcgaa ggcgaggagg tcgagatcct
     2461 gccgccgggt acgcgagcac gcatccgttc gttgcaaacg caccgcaccg cgcgaaccga
     2521 agccgtaccg accagccgtg tcgccctgaa cctggcgggg ctggaccggc acgaaatccg
     2581 ccgcggcgac gcggtcgtcg cgcccggcgc ctggcgcgtc accaccacga tcgacgccga
     2641 gctgcacggg gtgcgttcgc tggaccatgc cgtcaccggc aaaggcgcgt tccttctgta
     2701 tgcgggcacc gcggagacgc ctgcgcgcat ccgtcttctc gacgccgagc gaatcgaggc
     2761 cggcacgcga gccttcgcgc gcatcaccac caccgaaccg gtcgtggcgg caccgggcga
     2821 ccggatcgtg ttgcgcgata ccggccgccg gcagaccgtt gcgggcggaa ccgtgctcga
     2881 cgcgcacccg ccgcacggac gcaccggcga cgatcgcgtc gcgcagctga acgcgcgcgc
     2941 ggccgccgca accgagccgg cggtgctggc gacgctcacc gtcagcgaac gcggcgtcgt
     3001 cgcccgtgcc gatcgagcgt ggctgaccgc cgccgacccc ggcgacgccg tggggcttcc
     3061 gtcctattgg gtctctcgcg catggttcga cgcggccgcc aagcggcttc gcgacggact
     3121 gcggcgctac cacgcgcaca cgccgctgga gaaaggcatc ccgcgcgaca cgctccgccg
     3181 tgatgccggc atcggcgacg cgaagctgtt cgacgagatg ctcgacgcga tgaaagacga
     3241 gatcgaccag gacgctgcca tcgtgcgcct agccgggcac cgcgtcacgc tttcgcccga
     3301 gcaggacgcc gtgcgcgcga agctgatcgc cgagatggac gcgtcggggt tcagcccgcc
     3361 gccgctgtcc ggcctcgcct cgcgctacgg cgcggcgctg atcaaggcgc tcaccgacgc
     3421 cggcgcgctg atcaagatca acaccgacat cgcctatacg gccgagcggt tcgacgccgc
     3481 caaagcggca atcgccgatg ccgtgaacgc ggaaggtcct ctgacggcag gccggatcaa
     3541 agagatcctt cagacctcac gcaaatacgc gattccgctg ctcgagcggc tggatgcgga
     3601 gggctttacg cgccgcgacg gcgacgtacg gatcgttcgc tcctgagcgg atcgacctcg
     3661 gtccggccga cctcgttgcc tgccggatcc acgaaacgga actgcttgcg ggccgcggcc
     3721 ggctcgggcg gctgctgtcc gggatcgagc ggaaccatgt cggttccgaa tcgcatgccg
     3781 agcagcttcc acagctcgac cgcgcaccgg cggcacgata cccgcatcgc ggcggaatcc
     3841 gcgcgggtcg tcgccccgca ccagctgcac cagaacgtca ccatgagcac aacgcctctc
     3901 gcctcgtgtg ctccgtgaaa gcccctcacc ccggtgtgct tacaattgca agcatacccg
     3961 ggttcgtgtt gcctcacgca cacatcggcg tgacgttccg gtgaactttc cggccgttcc
     4021 taggacaatt cggcaatcgc ctcgacttcg acggtcgcgc ccatcgggag cgccgcgacc
     4081 ccgaccgccg cgcgcgcatg ccggcccgcg tcaccgaaca cctcgacgaa cagctcgctg
     4141 gccccgttgg cgacggtcgg ttgggcatga aagccctcgg atgaggccac gaacacggtg
     4201 accttgacga tgcgcgcaac gttgtcgagc gatccaccgg cggcgtcccg cagctgtgcg
     4261 agcacgttga ccgcgcacac gcgggcatca tcgacggcat cctccagcgt cacgtcgcgg
     4321 ccgaccaggc ccgtccgcgt cagcgcaccg tccctgaacg gcacctggcc cgacacgtag
     4381 acggtcgcgc ccgatacggc ggtgggcacg taggccgcta ccgccttcgg cggctcggga
     4441 aggaccagcc cgagcgagct cagcctctcc tcggcgctca tgactgcttc gggcgcttca
     4501 gcagcgcgac ggttccggga acgtcggtcg ggttgtagac ctgcaccagc tcccagcctt
     4561 cgtcgcccca gttgttgaga atctcttgaa tcgcgtgcgg gatcaacggc accgacgcgt
     4621 actcccattg cggcacggcg cacctccacg tcgaacggac gaatcgtcgg ggcagcctac
     4681 ccgacggctt ccgggaaaaa gaagcggccg gggtctcgtc agcagacccc ggccgcgtgg
     4741 atgacgccgc gttcgatgcc gggcgggagg ggggaagggc ctacccggct cgtttcgcca
     4801 tcaacctgcg gcgctccaat tcgttgaggc cgccccagat cccgtagggc tcgcgggtat
     4861 cgagggcgaa ctccaggcac tgcgtccgca cggggcactc gcggcaaatc gttttcgcgc
     4921 gtgcctcacg ggcaagcttc tcctctttca gctcgccggt cgcaggcgag aagaacagcg
     4981 tggcatcacg accgcgacag gcggcccggg actgccaccc gtcgtccgtc tcgtagaggt
     5041 tccgcaccgt gtccgttcgc atatcaacct ttcctcgctg ttatacgacg ccagacgagg
     5101 atgagatgtt cctgaaaagt gcccactgcg aatgtggcga aaaccttgcg attcgcttgg
     5161 atgatgccgg gtggcacgct gaccagggga tttaccgtga gaaatgatcc gaatggttgc
     5221 tcaggcgcgg cggacgcgaa gcaacgttcc ctcgaggccg gtcacgagca cgtcctcgcc
     5281 gacgcggatg cgctcgcctt cgtcggcgcg cgcgcgccac agggcatcgg cgacccagac
     5341 atagccttcg gggttgagga tgcttcgcac ctgaccggct tggccgacga gcgatgcgtc
     5401 ggcgttcttg cgaacgggct gccgcgcgcg gcgaacaagc gtcatgacgg gcacgaagaa
     5461 caccaaggcg gacaccacac ccaacagcgc gagccaccac gcaattcgaa gcggtgcggc
     5521 cgggccgggg aacagaccga acgagccggc ggagaacgcg accgcgccga cggcggtcgg
     5581 cacgcccagc ccgtcgagct tgacgtcgag cgtcatcaga atcgttccgc cggtgagcgc
     5641 gatcaccgcc cacagcgacg acggcaggac cgtcaggccg tagacgccgc cgaccagcag
     5701 cagcgcgccc gtgacgccgg cgacgccgaa gccgggctgg aacagctcga aggcgatcag
     5761 cagcgcgccg gcgacgagca ggatgtagac gagcgaggca ttggcgagcg tgtggagcac
     5821 gcgcggccag gggccgggct tgtggaagcg gatgtccacc gcatccttgt ccaagttgag
     5881 cgtgcgcgag ccggcggcgg tcgtgacctc gcggccgttc gcgcgctgaa gcagctcggc
     5941 gaccgacacg accgtgtagt ccacctgcct gcggtcgagc gcgaccgagg ccgggatggc
     6001 ctcgtccagg tagatcgccg gtgcgcgtcc gcgcgccgcg gcgagctcgt tgagctgctc
     6061 gagcgtcgcg gtgcgcaggg cacggccacg gtcgctgtcg gccttgaccg cgagattcgt
     6121 cgggtgggcg ggaccgagcc gtgccgacgg gccgatcgcc gcgatgtgcg cggcagcggt
     6181 cagaaccgcc gcggaccctc ccgcgacggc acgctgcggc ccgacgtaaa ccgcaaccgg
     6241 cacgcgcgag gcctcgatca ggcgaagcag cgactcggtg cgcgtgttga gcccgccgtc
     6301 ggaggagatc gagatcacca gcagctcggc cttctccttg ttggcctcgc ggatctcgtc
     6361 ggcgatgtac tcggccatcg gcggatcgat cagcccggag atctcgatga tgtccaccac
     6421 cggcgcatcg tctttctggg catgcggaac ggccgccccc gtttcggcgc gcgccagcgc
     6481 cgggaacagc aagggcagcg agagaaggaa ggccgcgagc gcggccgctc tgaaccgcat
     6541 tccggggcgg gattatagga cgcgtccgcg gcggccgctt cgcgcgggcc tggcgggggt
     6601 ctaggatgaa tcccacatga ccgcacaagc cgccaaactc gccaccgcct cgctgaccga
     6661 gtcgcggctg atcgtggtct cgggcaaggg cggcgtcggc aagaccaccg tcgccgccgc
     6721 gatcgcgacc gccgccgcgg ccgccggaaa gaaagtgctg ctggccgagg tcgagcagcg
     6781 tgacgggctg gcgccgctgt tcggacggcc ctcgatgagc tacgccgagg tttcgctggc
     6841 acagaacatc accgggatcg cggtcgtgcc ggacgaggcc ctcaccgagt acctccagct
     6901 cttctacggc atcggccgga tcgcccggcc cctgctgacc ggcaaggcgg tcgagttcgc
     6961 gaccagcacc gcgccgggtc tgcgcgacat cctgctgatc ggaaagatca aagaggcgga
     7021 gaaccgccgc accggcggca tgtacacctt tgatctgatc gtgctcgacg cgccgccaac
     7081 cggccgcctt ccccggttcc tggacgcgcc tcgcgcggtc gtggagctgg ttcagtcggg
     7141 cgcgatccgg cagcaggcgc aaggggtgct cgacatgctg accgatccgc gacgcacccg
     7201 ggtcgtgttg gtgacgctgc ccgaggagat gccggtgcgc gagaccatcg aaggcgcggg
     7261 cacgatgcgg gagatgggca tcgcgctcgg cccggtgatc gtcaacggcc tgtcgccgca
     7321 gccgttcaag accggccagc agcgcgcgct cgccaaggac ggcgccgcgg cgctactcgc
     7381 cgaggccggc gcggccggcg tggacctgtc ggaggaagcc gcgctcggcg tggcctcggt
     7441 cgccaacgcg cacgctcgcc gcgccgcgaa tcagcgtcgc gcgctcaaag agctcaccga
     7501 cgagctcgac ctgtcccaga tccggctgcc ggcgctgttt acgccgcaga tcggccccgc
     7561 cgagcggcgg cgtctggccg acgagctcgt cgcgcaggga gcgatcgcat gaacgacctc
     7621 gccgagatcc tgcgcgagca gcacgtgatc gtgtgcac
//
LOCUS       DATLFO010000216         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_94470, whole genome shotgun sequence.
ACCESSION   DATLFO010000216 DATLFO010000000
VERSION     DATLFO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94470"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1489
                     /gene="gcvPB"
                     /locus_tag="VM841_08830"
     CDS             20..1489
                     /gene="gcvPB"
                     /locus_tag="VM841_08830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HVL90323.1"
                     /translation="MRGAIGTQDEALVFEQSRPGRQASSFEPLDVPGAPADAIPEGLR
                     RAKPPVLPEVAERDLVRHYTRLSHRNYGIDLGFYPLGSCTMKYNPKVCDEIAAMPGFR
                     RLHPLQPESQTQGTLEILVELEEALCEVTGMSGVTFQPAAGAHGELTGLLLMRAWHAH
                     HGNDKRRIIIPDSAHGTNPASVTFAGYEVATVPSNRNGLVDIDALEAMVGDDVAGLMI
                     TNPNTLGLFERNIGRIAELIHGVGGLLYYDGANLNAILGRARPGDMGFDIVHMNLHKT
                     FATPHGGGGPGAGPVAVTEPLVPFLPGPRPVRDGDTYRWDTLDARSIGRMHTFHGNAA
                     VLVRAAAYIKAHGREGLHRISERAVLNANYLAKKLEGVYDLAFPAEPPMHEFVLTARK
                     QKAKGAKAMDVAKRLIDYGYHPPTVYFPLIVDEAMMCEPTETETPETLDGFAQAMIEV
                     DREITENPDVFPAAPTTTPVSRPDEAKAARDLKPRWYPG"
     gene            1489..2211
                     /locus_tag="VM841_08835"
     CDS             1489..2211
                     /locus_tag="VM841_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL90324.1"
                     /translation="MDSHDRRQVEFYERTAGRYDRSVWSLGCRDNRNHETKIRAVAEA
                     ISADGGGRVLEVGAGTGLHGRWLLEHTPVEHTGLDLSEPMLRIAAGRLARFAGRSRLT
                     IGDALDLPFPDAAFDAAFCVGTLHHLPSAEAGMRELARVVKPGGRVAALEPNWKFPSV
                     LVYSAITPEERKTFAINDRRLVAWARRAGLTEVRLQRLLYTPPKPVRWHRTFDRIDQA
                     LARTPGLRQLSINLLVAGRRPA"
     gene            complement(2265..>2611)
                     /locus_tag="VM841_08840"
     CDS             complement(2265..>2611)
                     /locus_tag="VM841_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL90325.1"
                     /translation="REVFAAMCGVRVATVIDIEKGRQMPGGGTLMRLAASLKCRVEDL
                     MPPGYQVTFGPLMVPRERPVESGKQTTRRRKAKESGFAAVAQMVAVSASMLAFLALPG
                     VTKAVTPIIHLM"
BASE COUNT          437 a          927 c          866 g          381 t
ORIGIN      
        1 cgtgtaaggc ggtggcgtga tgcgaggcgc gatcggaacg caggacgagg cgctcgtctt
       61 cgagcagtcg cggccgggac ggcaggcatc gtccttcgag ccgctcgacg tgccgggggc
      121 acccgcggac gcgatccccg aaggattgcg ccgcgcgaag ccgccggtgc tgcccgaggt
      181 cgccgagcgc gacctggtgc gtcactacac gcgcctgtca catcgaaatt acggcatcga
      241 tctcggcttc tatccgctgg gctcgtgcac gatgaagtac aacccgaagg tgtgcgacga
      301 gatcgccgcg atgccgggct tccggcgcct gcatccgctg cagccggaat cccagacgca
      361 gggcacgctc gagatcctgg tcgagctgga agaggcgctg tgcgaggtca ccggcatgag
      421 cggcgtaacc ttccagcccg cggccggcgc gcacggcgag ctgaccggct tgctgctgat
      481 gcgcgcatgg cacgcgcacc acggcaacga caagcggcgc atcatcatcc cggactcggc
      541 ccacggcacc aaccccgcgt cggtcacctt cgccggctac gaggtcgcga ccgtgccgtc
      601 gaacaggaac ggcctggtgg acatcgatgc gctcgaggcg atggtcggcg atgacgtcgc
      661 aggcttgatg atcaccaacc cgaacacgct gggactgttc gagcgcaata tcggccggat
      721 cgccgagctg atccacggcg tcggcgggct gctctactac gacggcgcga acctcaacgc
      781 gatcctgggc cgcgcgcggc cgggcgacat gggcttcgac atcgtgcaca tgaacttgca
      841 caagaccttc gccacaccgc acggcggcgg aggacccggc gccggcccgg tcgcggtgac
      901 cgagccgctc gtgccgttcc tgcccggtcc gcggccggtg cgcgacggcg acacgtatcg
      961 ctgggacacg ctcgacgctc gctccatcgg acgcatgcac accttccatg gcaacgcggc
     1021 cgtgctcgtc cgcgccgccg cctacatcaa ggcgcacggc cgcgaagggc tgcaccgcat
     1081 cagcgagcgc gccgtactga acgccaacta cctcgccaag aagctcgaag gcgtctacga
     1141 cctcgcgttc cccgccgagc cgccgatgca cgagttcgtg ctgaccgcgc gcaagcagaa
     1201 ggccaaaggc gcgaaggcga tggacgtggc gaagcggctc atcgactacg gctaccatcc
     1261 gccgaccgtc tacttcccgt tgatcgtgga cgaagcgatg atgtgcgagc cgaccgaaac
     1321 cgagacgccg gagacgctcg acggcttcgc gcaggcgatg atcgaggtcg atcgcgaaat
     1381 caccgagaac cccgacgtgt ttccggccgc gccgaccacc acgcccgtgt cgcgtcccga
     1441 cgaggccaag gccgctcgcg atctgaagcc gcgctggtat cccggctagt ggactcccac
     1501 gaccgccggc aggtcgagtt ctacgagcga acggccggcc gctacgaccg gtcggtgtgg
     1561 tcgttgggct gccgcgacaa ccgcaaccac gagaccaaga tccgcgcggt cgccgaagcg
     1621 atcagcgccg acgggggagg gcgggtgctc gaggtcggtg ccggcaccgg cttgcacggt
     1681 cgctggctgc tcgagcacac gccggtcgag cacaccggct tggacctctc cgagccgatg
     1741 ttgcggatcg cggccgggcg gctcgcccgg ttcgcgggac ggtcgcggct cacgatcggg
     1801 gatgcgctgg atctgccgtt cccggacgcg gccttcgacg cggccttctg cgtcggcacg
     1861 ctccatcatc tgccgagcgc cgaggccggc atgcgcgagc tcgcgcgcgt ggtgaagccc
     1921 ggcggccggg tcgcggccct cgaaccgaac tggaagttcc cgagcgtgct ggtgtactcg
     1981 gccatcacgc cggaggaacg caagacattc gccatcaatg accgccgcct cgtcgcctgg
     2041 gcccgccgag cgggcctgac cgaggtccgg ctgcagcggc tgctctacac gccgccgaag
     2101 ccggttcgct ggcaccgaac gttcgaccgc atcgatcagg ccctcgcccg aacgcccggc
     2161 ctgcgccagc tctcgatcaa cctgctcgtc gcgggacgac ggccggccta gaagcccgag
     2221 caccacgagc cgagtgcggc acctcctggc cgtctctatg aagtttacat aagatggatt
     2281 atcggcgtga ccgctttcgt cactcccggc agggcgagaa atgccagcat agaggcactg
     2341 acggcgacca tctgcgcgac cgccgcgaaa cccgactcct tcgctttccg tcgccttgta
     2401 gtttgcttgc cggactcgac tggccgttcg cgtggaacca tgagcggccc gaacgtgacc
     2461 tgatagcccg gcggcatcag gtcttcgacg cggcatttga gcgacgcggc gagccgcatg
     2521 agcgtcccgc cgcccggcat ctgccgaccc ttctcgatgt cgatgaccgt cgcgacccgc
     2581 acaccgcaca tggccgcgaa cacttcccgg t
//
LOCUS       DATLFO010000217         6804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_4378, whole genome shotgun sequence.
ACCESSION   DATLFO010000217 DATLFO010000000
VERSION     DATLFO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VM841_08845"
     CDS             <1..187
                     /locus_tag="VM841_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90326.1"
                     /translation="PHTGSSAVGDMPAVDEIVRAVESRAITLLPGRVRLALGREPSEE
                     HARRRQRVRGAALVRYR"
     gene            215..367
                     /locus_tag="VM841_08850"
     CDS             215..367
                     /locus_tag="VM841_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90327.1"
                     /translation="MAIALLEISFIVLLVGMLGLAGVIGMVVVVRLVEPRGTKAFLQR
                     LRSTRR"
     gene            376..1536
                     /locus_tag="VM841_08855"
     CDS             376..1536
                     /locus_tag="VM841_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90328.1"
                     /translation="MALNFEFTEEQLAFKKSVKEFAQKVVAPGADQRDKDGHFDREVW
                     NAVGAFGLCGLPIPEEYGGSGADIVTTNLAFEALEEGGRDSGLLLSLGAHVCIGAVPI
                     WLHGTEEQKRKYLPKLCSGEWIGAFAITEPDAGSDAAGIKTTARREGDGWVLNGSKTF
                     ITNGPVADVISVVAVTDPDAPSGNRMSCFVIEPPTDGFEVGKELDKMGNRSSPTSELH
                     FRDCFVPAGRMLGPEGSALWKVAFECFDWERTVMVASAIGGMEAQLAACIEYARERKA
                     FGKPISQFEMIQDKLARMRVSINASRWLLYHAAWLKQEGKPHMQEASIAKYFVAEAAV
                     QNALEAVQIFGGYGYIKEYPVERSIRDAKLATIGGGTSEIQKLIIARTLLGE"
     gene            complement(1544..2326)
                     /locus_tag="VM841_08860"
     CDS             complement(1544..2326)
                     /locus_tag="VM841_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90329.1"
                     /translation="MPSVRVGDADIYYETHGEGDPVVWIMGLGLDSRMMMMFTAAFPQ
                     YRNVVIDNRGTGRSSVPPGPYSMDQMSADVLTVMDDLQIEQARVIGMSLGGAIAQHVA
                     VQAPQRVRSLTLCCTWAGPNEWIRRLNELALLEAEAVGYEAILKHTLLLLFSPKFIIE
                     RPEMVQMFEDMGKQMVAPMEPFFSQIAAAMSHDVREKVKTLSVPTMIMAGRRDTFVPP
                     ELSDELHALIPGSTLKVYEGGHAFMLEDAQSFNADLTAWLAQ"
     gene            2411..3265
                     /locus_tag="VM841_08865"
     CDS             2411..3265
                     /locus_tag="VM841_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90330.1"
                     /translation="MSEAVFVDLGARPVWRVAGPAGMKFLDNVLTADLDDVTAGRGAI
                     AALLTVKGRIRTIVRVLATAGGAALVECEPAAAGGVEEGLVRIAPLGGTEMERSDRHL
                     LRFIGEVPPDLSPGGAEHSHAERDGAILIRTVWGGAGVDILAEPERAAEWIEVLSRHA
                     RAATAGDLEPIRIRDAFPRFGVDIDDSTHVLETTLPGHGAVADAKGCYPGQESVAKIR
                     NLGHVRRRMVALEATGAAPSPGDPVCSEAGDEVGRVTSVAGSMALAIVKVDAGALTVG
                     GAPASIVS"
     gene            complement(3262..3735)
                     /locus_tag="VM841_08870"
     CDS             complement(3262..3735)
                     /locus_tag="VM841_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90331.1"
                     /translation="MRFVAIVAAAGLVAGSAFGVLSRQAVARNPTAAESAARPDIGTG
                     ERLLLVIAGEYATRAQADAAAAGIVLGDLQGFVVAATSDFEGMTPGRYLVMSAFRTAQ
                     GAAEFEELVTMAGYGPMRRAVAVYRGSSWIGLGQEASPDGSGPLRGPLAPPEPNP"
     gene            complement(3744..4319)
                     /locus_tag="VM841_08875"
     CDS             complement(3744..4319)
                     /locus_tag="VM841_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVL90332.1"
                     /translation="MIRRALIAAVTAAAAFAGGAVAAAPLGYLGKVESDSAWQDRHEG
                     YPAVPVNEATLRAVFGPPCSADTHANRYWLVAQDDGKPYRVNFHKKLGGTASSNLDND
                     IPGHMHRSGLDAKLKRGIWGYACRMKRGSMTQYSVHAWGVAVDVNSAHERPGSACRTV
                     PDALGAIWTSHKWKWGKAWNDCMHFQYATGY"
     gene            4553..5875
                     /locus_tag="VM841_08880"
     CDS             4553..5875
                     /locus_tag="VM841_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90333.1"
                     /translation="MTGKAAASATASSPAATSGAGAPDPGVVSSIFRPEHEALRASVR
                     AFVDKELAPHADQWEADGHFPDWVFEKLGAAGFLGLHYPVEYGGQGGDYLSGLVLKEE
                     MARVGSGGVGMAVAVQTDMATPPIHKFGTEAQKQEYLVPAIKGTKIACLGITEPDAGS
                     DVANIQTVARREPSGDWIINGRKIFITNGVRAHFCTLVARTPGDATAAKPGVKGERDY
                     RGFSLFLVPTDVPGWSVARRLDKLGMHSSDTAEIAIDDVRVPADALLGVEGEGFPQIM
                     WELQGERLVGVAGTIAGSQLLLERCISYAKERTAFGKPIGAFQAVSHKIATMGTEIEA
                     AKQLIYEAAYKFGRGEYPVKEISMAKLYGGIVASRVANEAMQIFGGAGYTMDLPVQRA
                     WRDTRLIRIGGGTDEIMREIIAKLSGYSSNARAFADPGATTSTQEGNQ"
     gene            5872..>6804
                     /locus_tag="VM841_08885"
     CDS             5872..>6804
                     /locus_tag="VM841_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90334.1"
                     /translation="MSEDARWVRPLFLSLAALVGLVVMVWGLINGAMGAFHIYEPRYG
                     RVEADQGDIANAVSSIYGPIFELGTVQGTTPPGSGEALARAARQAEQQALDRAKDRGY
                     NRAVTGALAFVLGGFILLVGSSGAKRSASRVARSMPASDRGPAPVPAAPVPRQTPPQQ
                     PPPRQQVPAQRPAPPPPPGRAVPVPPRPAGGTPSPVGRLQEAPAEAAPKRSTRKSSTP
                     KRAAKKKAVPKRKDTDTQPIQPAAQEPAAPQPPPAAPQQPPPAAPQQPPAAPGARPAA
                     PPPPPPRPMPVAPPPQTAPARPVTPPPAAPPPASP"
BASE COUNT         1039 a         2379 c         2360 g         1026 t
ORIGIN      
        1 accgcacaca ggctcgtccg ccgtgggtga catgccggcg gtggacgaga tcgtgcgcgc
       61 ggtggaatcg cgcgccatca cgctcctgcc ggggcgggtg cggctcgcgc tgggacgtga
      121 gccgagcgaa gagcacgctc gtcgtcggca gcgtgtgcga ggtgccgcgc tggtacgcta
      181 tcgctgaccg actcgaccgg aaggaccgac gaccgtggcc atcgcactgc tggagatctc
      241 cttcatcgtc ctgctcgtcg gcatgctcgg cctcgccggc gtgatcggca tggtcgtggt
      301 ggtcaggctc gtcgagccgc gcggaaccaa ggccttcctg cagcgactcc gctcgacacg
      361 ccgctaggcc ggctcatggc cctcaacttc gagttcaccg aagagcagct cgctttcaag
      421 aagtccgtca aggagttcgc gcagaaggtc gtcgcgccgg gcgcggatca gcgcgacaag
      481 gacggccact tcgaccggga ggtctggaac gccgtcggtg cgttcggtct gtgcggcctg
      541 ccgatcccgg aagagtacgg cggcagcggc gccgacatcg tcaccacgaa cctcgcgttc
      601 gaggcgctcg aggagggcgg ccgtgattcg gggctgctgc tgtcgctcgg cgcgcacgtg
      661 tgcatcggcg cggtgccgat ctggctgcac ggcaccgagg agcagaagcg caagtatctg
      721 cccaagctgt gcagcggcga gtggatcggc gcgttcgcga tcaccgagcc cgacgcgggc
      781 tctgacgccg ccgggatcaa gaccaccgcg cggcgcgaag gcgacggctg ggtgctcaac
      841 ggatccaaga ccttcatcac caacgggccg gtcgccgacg tgatcagcgt cgtggccgtc
      901 accgatccgg acgcgcccag cggcaaccgc atgtcgtgct tcgtcatcga gccgcccacc
      961 gacggcttcg aggtcggcaa ggagctcgac aagatgggca accgctcctc gcccacctcc
     1021 gagcttcact tccgcgactg cttcgtgccc gccggccgga tgctcggccc ggaaggctcc
     1081 gcgctctgga aggtcgcgtt cgaatgcttc gactgggagc gcaccgtgat ggtcgcctcc
     1141 gcgatcggcg ggatggaggc gcagctcgcc gcatgcatcg agtatgcgcg cgagcgcaaa
     1201 gcgttcggca agccgatctc gcagttcgag atgatccagg acaagctcgc gcgcatgcgc
     1261 gtcagcatca acgcgtcccg ctggctgctc taccacgcgg cctggctcaa gcaagaagga
     1321 aagccgcaca tgcaagaagc ctcgatcgcc aagtacttcg tcgccgaggc ggccgtgcag
     1381 aacgcgctcg aggcggtgca gatcttcggc ggctacggat acatcaagga gtatccggtc
     1441 gagcgctcga tccgcgacgc gaagctcgcc accatcggcg gcgggacgag cgagatccag
     1501 aagctgatca tcgcccgcac tctgctcggc gaatagcccc gcgttactgc gcgagccagg
     1561 ccgtcaggtc cgcgttgaag ctctgtgcgt cttcgagcat gaacgcgtgg ccgccttcgt
     1621 agaccttgag tgtcgatccg gggatcagcg cgtgcagctc gtcgctgagc tcgggcggaa
     1681 cgaacgtgtc gcggcgaccg gccatgatca tcgtcggaac cgagagcgtc ttgacctttt
     1741 cacgcacgtc atggctcatc gccgcggcga tctggctgaa aaacggctcc atcggcgcta
     1801 ccatctgctt gcccatgtcc tcgaacatct ggaccatctc cggccgctcg atgatgaact
     1861 tcggggagaa cagcagcagc agggtgtgct tgaggatcgc ctcgtagccg acggcctcgg
     1921 cctccaagag cgcgagctcg ttgaggcggc ggatccattc gttcggcccg gcccaggtgc
     1981 agcacagcgt cagcgaccga acccgttgcg gcgcctgcac cgccacgtgc tgcgcgatcg
     2041 cgccgccgag cgacatgccg atcacacgcg cctgctcgat ctggagatcg tccatgacgg
     2101 tcaggacgtc ggccgacatc tgatccatcg agtagggccc cggcggcacc gacgagcggc
     2161 ccgtgccccg gttgtcgatc accacgttcc ggtactgagg gaacgcggcc gtgaacatca
     2221 tcatcatccg ggagtccagg cccagaccca tgatccatac gaccggatcg ccctcgccat
     2281 gggtctcgta gtagatgtcg gcgtcgccga cccggactga gggcatgaaa tcctcctggt
     2341 ttcgcgttcc tctcaccgta tccgatggag cccggttgcg tcggccccgc cggcgaggga
     2401 aggtagggcc atgagcgaag cggtcttcgt cgatctgggc gcgcggccgg tgtggcgggt
     2461 cgcggggccg gccggcatga agttcctcga caacgtgctg accgccgatc tcgacgacgt
     2521 caccgcaggc cgcggggcaa tcgcggcctt gctgaccgtc aaaggccgca tccgcacgat
     2581 cgtgcgcgtg ctggcaaccg ccggcggcgc cgcgctcgtc gagtgtgagc ccgccgccgc
     2641 cggcggcgtc gaggagggcc tggtccgcat cgcgccgctc ggcggaaccg agatggagcg
     2701 atccgaccgt cacctgcttc gtttcatcgg ggaggttccg ccggaccttt ccccgggcgg
     2761 cgccgagcat tcgcatgcgg agcgggacgg cgcgatcctg atccgaaccg tttggggcgg
     2821 cgccggtgtc gacattctgg ccgagccgga gcgcgccgcc gagtggatcg aggtcctgtc
     2881 gcggcatgcc cgcgcggcga ccgccggcga tctcgagccg attcgcatcc gggacgcgtt
     2941 cccgcgcttc ggcgtggaca tcgacgactc cacgcatgtg ctcgagacga ccctgccggg
     3001 gcacggcgcc gtcgccgacg ccaagggctg ctatcccggg caggagtccg tcgccaagat
     3061 ccgcaacctc ggccacgttc gcaggcgcat ggttgcgctc gaggccaccg gcgcggcgcc
     3121 ttcgcccggc gatccggtgt gcagcgaggc cggcgacgag gtcggtcgcg tcaccagcgt
     3181 cgccggatcg atggcgctgg cgatcgtgaa ggtggatgcc ggcgcgctca ccgtcggcgg
     3241 cgcgccggcc tccatcgtct cctaaggatt gggctccggc ggcgcgagcg gcccgcgcag
     3301 cggtcccgat ccgtccgggc tggcctcctg gcccagtcca atccagctcg agccccggta
     3361 gacggcgacc gctcgccgca tcggcccgta gccggccatg gtgacgagct cttcgaactc
     3421 ggccgcgcct tgtgcggttc ggaaggcgct catcacgagg tagcgtcccg gcgtcatccc
     3481 ctcgaagtcg ctcgtcgcgg cgacgacgaa cccctgcagg tcgccgagca cgatcccggc
     3541 cgcggccgcg tcggcctgcg cgcgggtcgc gtactccccg gcgatcacga gcaggagccg
     3601 ctcgcccgtg ccgatgtcgg ggcgtgccgc gctctcggcc gcggtcgggt tgcgcgcaac
     3661 ggcctgccgg ctcaggaccc cgaatgccga gccggccacc aggccggctg cggcgacgat
     3721 cgctacgaat ctcatagctc ctcctagtag ccggtcgcgt attggaagtg catgcagtcg
     3781 ttccatgcct tgccccactt ccacttgtgc gacgtccaga tcgcgccgag cgcgtcgggc
     3841 acggtccggc aggcggatcc gggccgctcg tgcgcggagt tcacgtccac ggccacgccc
     3901 cacgcgtgca cgctgtactg ggtcatcgag ccgcgcttca tgcggcacgc gtatccccag
     3961 atgccgcgct tgagctttgc gtccaggccc gagcggtgca tgtgtccggg gatgtcgttg
     4021 tccaggttcg acgaggccgt gccgcccagc ttcttgtgaa agttgacgcg gtacggcttg
     4081 ccgtcgtcct gggcgacgag ccagtagcgg ttggcgtggg tgtcggcact gcagggaggg
     4141 ccgaacaccg cccgcagcgt cgcttcgttc accggaaccg ccgggtatcc ctcgtggcga
     4201 tcctgccagg cggaatccga ctcgaccttg ccgaggtagc cgagcggggc cgccgccacc
     4261 gcgccgcccg cgaaggccgc agcggccgtg accgccgcga tcagagcacg tcgaatcatg
     4321 gtcatctcct ttcgggaagg cgaagggggc gggaagggga acggatggcg cgctgcgcaa
     4381 cgcatcggcg acgctacggc cctgcggacc gggggtccac aagcggcaaa ccgtgcatat
     4441 cggcgcgatg gtatcgatcg atccgctcgg gctgattcgc ggcttcgagt gcgccgcccg
     4501 atccgggcgc gtcctcgcat cgggcctgtt cgcggcatat gatccgggcg ccatgaccgg
     4561 gaaagctgcg gcgtcagcca ccgcgagttc gcccgccgcg accagcggcg ccggcgcgcc
     4621 cgaccctggc gtggtctcgt ccatcttcag gccggagcac gaggcgcttc gtgcctcggt
     4681 ccgggcgttc gtcgacaagg agctcgcccc ccacgccgac cagtgggagg ccgacgggca
     4741 tttccccgac tgggtcttcg aaaagctcgg ggcggccggc ttcctgggtc tgcactatcc
     4801 ggtcgagtac ggcggccagg gcggcgacta tctgtccggg ctcgttctga aggaagagat
     4861 ggcccgggtc ggctcgggcg gcgtgggcat ggcggtggcg gtccagacgg acatggccac
     4921 cccgccgatc cacaagttcg gcaccgaggc gcagaagcag gagtaccttg ttcccgcgat
     4981 caagggaacc aagatcgcct gcctcggcat caccgagccc gacgccggca gcgacgtggc
     5041 gaacatccag accgtcgcgc gccgcgagcc gtccggcgac tggatcatca acggacgcaa
     5101 gatcttcatc accaacggcg tgcgcgctca cttctgcacg ctggtcgcgc gcacgcccgg
     5161 agacgccacc gccgcgaagc cgggcgtgaa aggcgagcgc gactatcgag gcttctcgtt
     5221 gttcctggtg ccgaccgatg tgccgggctg gagcgtcgcg cggcggctcg acaagctcgg
     5281 gatgcactcg tcggacaccg ccgagatcgc gatcgacgac gttcgcgttc cggccgacgc
     5341 gctgctcggc gtcgaaggcg aaggcttccc gcagattatg tgggagctgc agggtgagcg
     5401 gctcgtgggg gtcgcaggaa cgatcgccgg ctcgcagcta ctgctcgagc gctgcatttc
     5461 gtacgcgaaa gaacgcacgg cgttcggcaa gccgatcggc gcgttccagg ccgtgtcaca
     5521 caagatcgca acgatgggaa ccgagatcga agcggcgaag cagctcatct acgaagccgc
     5581 ctacaagttc ggccgcggcg agtatccggt caaggagatc tcgatggcca agctctacgg
     5641 cggcatcgtg gcctcgcgcg tggcgaatga agcgatgcag atcttcggcg gcgccggcta
     5701 cacgatggac cttccggtcc agcgtgcgtg gcgtgatacg cggctgattc gcatcggtgg
     5761 cggtaccgac gagatcatgc gtgagatcat cgccaagctt tccgggtatt cgagcaacgc
     5821 gcgcgcgttc gccgatcccg gcgcgacgac ttcgacacag gaggggaatc aatgagtgaa
     5881 gacgcccgct gggtgcgacc gctgtttctg tcgctcgcgg cgctggtcgg cttggtcgtg
     5941 atggtgtggg gcctgatcaa tggcgccatg ggcgcgttcc acatctacga gccacgttac
     6001 ggccgtgtcg aagccgatca gggagacatc gcgaacgcgg tgagcagcat ctacggaccg
     6061 atcttcgagc tcggcaccgt gcaggggacg acgccgccgg gatccggcga ggcgctcgcg
     6121 cgcgcggcgc gtcaagccga gcagcaagcg ctcgatcgtg ccaaggaccg cggctacaac
     6181 cgcgcggtga ccggtgcgct cgccttcgtg ctcggcggat tcatcttgct ggtcggctcg
     6241 tccggcgcca agcgctcggc gagccgggtc gcgcggtcca tgcccgcgtc cgaccggggg
     6301 cccgcgccgg tcccggccgc gcccgtgccg cggcagacac cgccgcagca gccgccgccg
     6361 cgtcagcagg ttcccgcgca gcggcccgca ccgccgccgc cacccggccg tgcggtgccc
     6421 gtgccgccgc gcccggccgg cggaacgccc tcgcccgtcg gccggcttca ggaggcgccg
     6481 gccgaagcgg ctccgaagcg gtccacccga aagtcctcca cgccgaagcg cgccgcgaag
     6541 aagaaggccg ttcccaagcg gaaggacacc gacacgcagc cgatccagcc ggccgcgcaa
     6601 gagccggcgg cgccgcagcc gccgcccgcc gcgccgcagc agccgccacc cgccgcgccg
     6661 cagcagccgc cggccgctcc gggcgcgcgc cctgcggccc cgccgccgcc gcctccgcgt
     6721 ccgatgccgg tcgcgcctcc gccgcagacg gctccggccc gtccggtgac tccaccgccg
     6781 gccgcgccgc cgccggcgtc gccg
//
LOCUS       DATLFO010000218         8486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_35296, whole genome shotgun sequence.
ACCESSION   DATLFO010000218 DATLFO010000000
VERSION     DATLFO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35296"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VM841_08890"
     CDS             <1..1019
                     /locus_tag="VM841_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVL90335.1"
                     /translation="ILLFMLGGVSQIGRDAEGPKQEYLVALAGPLASLMIAGVLSMLA
                     VASGGSFTDAASGTGVWATLAAVNLLLAMFNLIPGFPLDGGRVLRATIWAVTNDRIRA
                     TKMAANGGRIVAGGFVAGGVAYLLWASQGVGDFFPGVLYIALGWFLYSHASFAGNEDV
                     HREMLREQARREAMATAPPQPPARDIVPGSDAPLPPRIPARVTPKAPAATLDGPAAGP
                     AAPKRPAARAPAKKPAKKPAKKPAAAAKKAAGKAAERAAPKPPAGQAPTKKPAARTLT
                     APAKKAKKTDEGDAAKSEARARRGGRAKRPAASAEAGPEPRDGARDSRRRPPHARRGA
                     RGER"
     gene            1036..1995
                     /locus_tag="VM841_08895"
     CDS             1036..1995
                     /locus_tag="VM841_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90336.1"
                     /translation="MISGGQRTVAKCKRCGERARVEVARANAAFCNDCFVRYVDGQVQ
                     RAVADYEMFSETDRLLVCVSGGKDSLALWDILNRMGYDTTGYYISLGTGKEYTEESRE
                     MCERFAASRGLKLIVTDLAADEGFTIEEISKDSRRVPCSACGLAKRYLTNVTAAENGF
                     DVLVTGHNLDDEAATLMGNTLHWNTEYIARQRPSLPASADGFVRKVKPLVRVSERESA
                     AYCVIKGIDYVLEECPLVAGNTQLRYKDALNDLERRSPGTKQQFLFGFLDKAAPLFDA
                     AERPPLGRCRDCRQPTTGDLCAFCRLRGQVARKRAKTRAGDDA"
     gene            1992..2771
                     /locus_tag="VM841_08900"
     CDS             1992..2771
                     /locus_tag="VM841_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HVL90337.1"
                     /translation="MSAPLEAGERILLVDRKGRRYLLALKAGGEFHAHHGVVAHDEII
                     GKPEGIKVTSTGGATMIAYRPGIADFILKMPRGAQVIYPKDIGPILIEADVYPGATVL
                     EAGTGSGALTLALVRAVGEQGRVVSYDLREDHSAKARTNLESFLGKLPDSLELRIGDV
                     ISYEGEPADRVVLDLPEPWRAVAVAQRLLRRGGIMCCYVPSITQIALTVESMREAGFE
                     EIRPFETMVRPWHVEGASVRPEHRMVGHTGFLVTGRLLGLA"
     gene            3033..4766
                     /gene="arc"
                     /locus_tag="VM841_08905"
     CDS             3033..4766
                     /gene="arc"
                     /locus_tag="VM841_08905"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HVL90338.1"
                     /translation="MAGAEREHEREVSTLRSQLKFLEEETALLRRRLSDSPGQVKLLE
                     EKLNEARTQLSQALAQNERMTSALKEARDQIVALREEVEKLTAPPSGFGVFLGINDDG
                     TIDISSSGRKLRVAVHPDIDPKSLQRGQELMLNEAMNVIGVRTFEQQGEVVTLKEIID
                     ATRALVVGHTDDEKVVLLSDPLSSQHLRAGDSLLVDPRSGYAYERLPKPEVEELVLEE
                     VPDVSYDEIGGLKQQIEAIYDAVELPFLHAELFNEHELKPPKGVLLYGPPGCGKTLIA
                     KAVANSLAKKVADKYGRPEARSFFLNIKGPELLNKYVGETERQIRLIFQRAKEKSEEG
                     FPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVETLSNVIVIGASNREDMI
                     DPAILRPGRLDVKIKIERPDEHSAAEIFRIYLTSRLPIHPDEISQHGSAGVAVENMIQ
                     RTVQAMYADTDDNRFLEVTYANGDKEILYFKDFNSGAMIENVVARAKKEAIKRFLESG
                     VKGIKMDDLYESIRNEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLLGQDEGPGR
                     HIENLQPGQYL"
     gene            4854..6350
                     /gene="dop"
                     /locus_tag="VM841_08910"
     CDS             4854..6350
                     /gene="dop"
                     /locus_tag="VM841_08910"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HVL90339.1"
                     /translation="MSIPKVMGIETEFGITVPDAPEFNPVLASSILINSYAASASRRV
                     RWDYEEESPLRDARGFEAAVEMAPNIEPANDEELGLANVILTNGARYYVDHAHPEYST
                     PECTNPRELVVHDRAGERVLAESMSRAQSALSTGQRILVYKNNTDGKGASYGTHENFL
                     VDRACPFSRIVRDFTPFLVTRQIFAGAGMLGGGASNLPVPYQLAQRSDFFEVEVGLET
                     TLKRPIINTRDEPHADPQKYRRLHVIIGDANMSEVATYLKTGTASIVLRMIEDEAVST
                     EFLLENPVQAVRAISHDISCTKTVGLKDGRQVRAVDVQWGYFEMAQKYFARHEPDDWT
                     AAVMARWERVLSAIDDDPMQLDKEVDWVAKYKLIDSYRNRHGLPWDDQKLKMLDLQYH
                     DVRPDKGLAQVLYRSGKLERLTTDDEIERAITMPPRDTRAYFRGRCLAQFPGKISAAS
                     WDSVIFDTGGETLQRVPMMEPLRGTEAHTKALLDACRTAEELVDRLQG"
     gene            6394..6606
                     /locus_tag="VM841_08915"
     CDS             6394..6606
                     /locus_tag="VM841_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018723032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HVL90340.1"
                     /translation="MAKPSGGSEQKKKGTQKQDGGDDAGQASAEVIAKGEELKEDIDE
                     LLDEIDEVLETNAEEFIRSYVQKGGE"
     gene            6809..7630
                     /gene="prcB"
                     /locus_tag="VM841_08920"
     CDS             6809..7630
                     /gene="prcB"
                     /locus_tag="VM841_08920"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HVL90341.1"
                     /translation="MSELPIDWTKAAFSSDANPSFNDFVQRTAPVLLPQVTPGAGGMP
                     EQPHGTTILALTYADGVIIAGDRRATSGYSIADRKIEKVFPADDYSAVAIAGAAGPAI
                     DMVKLFRTELEHYEKVEGERLSLEGKANKLASMIKMNLPFAMQGFLVVPLFAGYDERR
                     QAGRIFRYDGIGGRYEESDYHADGSGGMHARGSLKGRWRRGLNREDAVLTAVEALFDA
                     AEEDVATGGPDLIRNIYPVVATVTAEGYRRVDDDEVGRAVDGAIADRREREERNR"
     gene            7627..8322
                     /gene="prcA"
                     /locus_tag="VM841_08925"
     CDS             7627..8322
                     /gene="prcA"
                     /locus_tag="VM841_08925"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HVL90342.1"
                     /translation="MSPMPYYVSPEQLMKDRADYARKGIARGKSIIAVECDEGILIVA
                     ENPSATLHKVSEIYDRIAFAAVGKYNEFENFRIAGVRLADLRGYAYDREDVSGRAIAN
                     AYAQTLGAIFTEQMKPYEVELLVAQVGSSQETDEMYRVMYDGSVSDEQGFMAMGGHAE
                     ALANDLQQGYRQQIGIGDAFTLAAKALAAADSGRTLTPANLEVAALERHRPRRTFRRY
                     SDEEVGELLAQGS"
BASE COUNT         1530 a         2825 c         2875 g         1256 t
ORIGIN      
        1 gtatcctgct gttcatgctc ggcggcgtct cgcagatcgg ccgtgacgcg gaagggccga
       61 agcaggagta tctcgtggcg ctcgccgggc cgctcgccag cctgatgatc gccggcgtgc
      121 tttcgatgct cgcggtcgcg agcggcggct cgttcaccga tgcggcgtcc ggcaccgggg
      181 tctgggcgac gctggcggcg gtgaacctgc tgctggccat gttcaacctg attcccgggt
      241 ttccgctcga cggcggccgc gtgctgcgcg ccaccatctg ggccgtgacc aacgaccgga
      301 tccgcgccac caagatggcc gcgaacggcg ggcgcatcgt cgcgggcggg ttcgtcgccg
      361 gaggcgtggc ctatctgctg tgggcctcgc aaggcgtggg cgatttcttc cccggcgtgc
      421 tctacatcgc gctgggatgg ttcctgtatt cgcacgcctc gttcgccggc aacgaggatg
      481 tgcatcgcga gatgctgcgc gagcaagccc gccgtgaggc gatggccacc gcgccgccgc
      541 agccgcccgc gcgcgacatc gttcccggat ccgatgcgcc tctgccgccc cggatcccgg
      601 ctcgggtgac accgaaggct ccggcggcta cgctggacgg gccggccgcc ggaccggcgg
      661 ctccgaaacg gccggcggct cgcgcaccgg cgaagaagcc ggcgaagaag ccggcgaaga
      721 agccggccgc cgccgccaag aaggccgccg gaaaagccgc ggagcgggcc gctccgaagc
      781 cgccggccgg gcaggccccg acgaagaagc cggccgcccg cacactcacc gcccctgcga
      841 agaaagccaa gaagaccgat gaaggtgacg ctgcgaaatc cgaagcgcgt gctcgacgtg
      901 gagggcgtgc gaagcgtccg gcagcttctg cagaagctgg acctgaaccc cgagacggtg
      961 ctcgtgattc gcggcgccga cctcctcacg cgcgaagagg agctcgcggc gagcgatgag
     1021 atcgagatcc ggccggtgat ctcgggcggg cagcgcacgg tcgccaagtg caagcgatgc
     1081 ggcgagcgtg cccgcgtcga ggtcgcccgc gcgaacgcgg ccttctgcaa cgactgcttc
     1141 gtgcgctacg tggacggcca ggttcagcgg gccgtggccg actacgagat gttctccgag
     1201 accgaccggc ttctggtgtg cgtgtccggc ggcaaggact ccctcgcgct gtgggacatt
     1261 ctcaaccgca tgggctacga cacgacgggc tactacatct cgctggggac cggcaaggaa
     1321 tacaccgaag agtcccgcga gatgtgcgag cgcttcgccg cgtcgcgcgg cctcaagctg
     1381 atcgtcaccg acctcgccgc cgatgaaggc ttcaccatcg aggagatctc caaggactcg
     1441 cgtcgcgtgc cgtgctcggc gtgcgggctc gcgaagcggt acctgaccaa cgtcaccgcg
     1501 gccgagaacg gattcgacgt gctcgtcacc ggacacaatc tcgacgacga ggccgcgacg
     1561 ctgatgggaa acacgctcca ctggaacacc gagtacatcg cccggcagcg gccgtcgctg
     1621 cccgcctcgg ccgacggttt cgtgcgcaag gtcaagccgc tcgtgcgtgt ctccgagcgc
     1681 gagtccgccg cttactgcgt gatcaagggc atcgactacg tgctcgaaga atgcccgctc
     1741 gtggcgggca acacccagct tcgctacaag gacgcgctca acgatctcga gcgccgttcg
     1801 cccggcacca agcagcagtt cctgttcggc ttcctcgaca aggctgcgcc gctgttcgac
     1861 gcggccgagc gcccgccgct gggacggtgc cgggactgcc gccagcccac taccggcgac
     1921 ctgtgcgcct tctgccggct gcgcgggcag gttgcgcgca agcgggccaa gacccgcgcc
     1981 ggggacgacg cgtgagcgcg ccgctggagg ccggcgagcg gatcctgctc gtcgatcgaa
     2041 aaggacgccg ctacctgctg gcgctcaagg ccggcggcga gttccacgcc caccacggcg
     2101 tcgtcgccca cgacgagatc atcggcaagc ccgaagggat caaggtcacc tccaccggcg
     2161 gcgcgacgat gatcgcctac cggccgggca tcgccgactt catcctgaag atgccccgcg
     2221 gcgcccaggt gatctaccca aaggacatcg gtccgatcct gatcgaggcc gacgtctatc
     2281 ccggggcgac ggtgctcgag gccggcaccg ggtcgggagc gctcacgctc gcgctggtac
     2341 gtgccgtggg ggagcagggc cgcgtcgtct cctacgatct gcgtgaggac cacagcgcca
     2401 aggcccgaac gaacctcgag tcgttcctgg gcaagctgcc ggactcgctc gagttgcgca
     2461 tcggcgacgt gatctcctac gaaggcgagc ccgccgaccg cgttgtgctc gatctgcccg
     2521 agccctggcg ggccgtggcg gtcgcgcaga gactgctgcg ccggggcggc atcatgtgct
     2581 gctacgtgcc ctcgatcacc cagatcgccc tcacggtcga gtcgatgcgt gaggccggct
     2641 tcgaagagat tcgccccttc gagacgatgg tgcgtccttg gcacgtggag ggcgcatccg
     2701 tgcggccgga gcaccgaatg gtcggccaca ccggctttct ggtcaccggc cggctcctcg
     2761 ggcttgcttg agcactcctg ttaacggctg ttaattactg aggtgacagc ctcaagacgt
     2821 ttccgcatgc cggcgaaaca ggaacgctcg gaagtagtta tcgaaatcat ccgaagtcgt
     2881 catgtccatg gctcgtccga ggcgttgatg taaatgaggg ccgttccacg aaccgataat
     2941 ccgagtgagg cgttgcacga gtggtgataa tgagcgtctg atacgagcga acggcggatt
     3001 cgccggacgc atcgacaacg gaggtttacg acatggccgg tgccgagcgg gaacacgaga
     3061 gggaggtctc caccctccga tcgcagctga agttcctcga agaggaaacg gcgctgctgc
     3121 ggcgacggct ctccgattcg ccgggtcagg tcaagctgct cgaggagaag ctgaacgagg
     3181 cccgcacgca gctgtcgcag gccctcgcgc agaacgagcg catgacctcc gcgctcaagg
     3241 aagcgcggga tcagatcgtg gcgctccggg aagaggtcga aaagctgacg gcgccgccgt
     3301 cggggttcgg cgtgttcctg gggatcaacg acgacggcac gatcgacatc tcctccagcg
     3361 gccgcaagct gcgcgtcgcg gttcacccgg acatcgaccc caagagcttg cagcgcggcc
     3421 aggagctgat gctcaacgaa gccatgaacg tcatcggcgt gcggaccttc gagcagcagg
     3481 gtgaggtcgt cacgctgaaa gagatcatcg acgcgacgcg tgcactggtg gtcggccaca
     3541 ccgacgacga aaaggtcgtc ctgctgtcgg acccgctgtc gtcgcagcat ctgcgcgccg
     3601 gcgactcgct gctggtagac ccgcgctcgg gctacgccta cgaacgcctg cccaagcccg
     3661 aggtcgaaga gctggtgctg gaagaggtgc cggacgtctc ctacgacgag atcggcggcc
     3721 tcaagcagca gatcgaggcg atctacgacg cggtcgagct gccgttcctg cacgccgagc
     3781 ttttcaacga gcacgagctc aagccgccca agggcgtgct gctgtacggc ccgcccggct
     3841 gcggcaagac gctgatcgcc aaggcggtcg ccaactcgct ggccaagaag gtcgccgaca
     3901 agtacggccg tcccgaggcg cgcagcttct ttctcaacat caaaggtccg gaactgctca
     3961 acaagtacgt cggcgagacc gagcggcaga tccgcttgat cttccagcgt gccaaggaga
     4021 agtccgagga aggcttcccg gtgatcgtgt tcttcgacga gatggactcg atcttccgca
     4081 cgcgcggatc gggcgtgtcc agcgacgtcg agaacaccat cgtgccgcag ctgctcagcg
     4141 agatcgacgg cgtcgagacg ctgtcgaacg tgatcgtgat cggcgcgtcc aaccgcgagg
     4201 acatgatcga cccggcgatc cttcgcccgg gccggctcga cgtcaagatc aagatcgagc
     4261 gccccgacga gcactcggcc gccgagatct tcaggatcta cctgacgtca cggctgccga
     4321 tccacccgga cgagatctcc cagcacggct cggccggcgt cgccgtcgag aacatgatcc
     4381 agcggaccgt gcaggcgatg tacgccgaca ccgacgacaa ccgtttcctc gaggtcacct
     4441 acgccaacgg cgacaaggag atcttgtact tcaaggactt caactctggc gccatgatcg
     4501 agaacgtcgt ggcgcgcgcc aagaaggaag ccatcaagcg gttcctcgag agcggcgtca
     4561 agggcatcaa gatggacgac ctgtacgagt cgatccgcaa cgagttcaag gagaacgagg
     4621 accttccgaa caccacgaac ccggacgact gggcgcggat ctcgggcaag aagggcgagc
     4681 gcatcgtcta cgtccgcacc ttgctcggtc aggacgaagg cccgggccgg cacatcgaaa
     4741 acttgcagcc ggggcagtat ctgtaggacg acagagcgta ggatgtccgg gccggtcgga
     4801 taggccggcc cggattcgtt gaggcggcga gtccgcctcg aaagggggcc atcgtgtcga
     4861 tcccgaaggt tatggggatc gagaccgagt tcggcatcac ggtgccggac gcgccggagt
     4921 tcaatcccgt gctggcctcg tccatcctga tcaactccta cgccgcctcg gcctcgcgcc
     4981 gcgtccgctg ggactatgag gaagagtccc cgctgcgcga cgcgcgcggg ttcgaggccg
     5041 cggtcgagat ggcgccgaac atcgagcccg ccaacgacga ggagctcggt ctcgccaacg
     5101 tcatcctgac caacggcgcg cgctactacg tcgatcacgc gcaccccgaa tactccacgc
     5161 ccgagtgcac caacccgcgc gagctcgtgg tgcacgaccg ggcaggcgag cgcgtgctcg
     5221 ccgagtcgat gagccgtgcg cagtcggcgc tgtccaccgg acagcgcatc ctggtctaca
     5281 agaacaacac cgacggcaag ggcgcgtcct acggcaccca cgagaacttt ctcgtggacc
     5341 gcgcctgccc gttctcgcgg atcgtccggg acttcacgcc gttcctggtg acccgccaga
     5401 tcttcgcggg cgcgggcatg ttgggcggcg gggcctcgaa tcttccggtg ccctaccagc
     5461 tcgcgcagcg gtcggacttc ttcgaggtcg aggtcggcct cgagacgacg ctgaagcgcc
     5521 cgatcatcaa cacgcgggac gagccgcacg ccgaccctca aaagtaccga cggctgcacg
     5581 tgatcatcgg cgacgcgaac atgtccgagg tcgcgaccta cctcaagacc ggcaccgcct
     5641 cgatcgtgct gcgcatgatc gaagacgagg cggtctcgac ggagttcttg ctcgagaacc
     5701 cggttcaggc cgtgcgcgcg atcagccacg acatctcgtg caccaagacc gtcggactca
     5761 aggacggccg ccaggtgcgg gcggtggacg tgcagtgggg ctacttcgag atggcgcaga
     5821 agtacttcgc gcgtcacgag cccgacgact ggaccgccgc ggtcatggcc cgctgggagc
     5881 gggtgctgag cgcgatcgac gacgacccga tgcagctcga caaggaagtg gactgggtcg
     5941 ccaagtacaa gctcatcgac tcgtaccgga accggcacgg gctcccatgg gacgaccaga
     6001 agctcaagat gctcgacctt cagtatcacg acgtgcgtcc cgacaagggc ctcgcgcagg
     6061 tgctgtaccg gtcgggcaag ctcgagcgtc tgaccaccga cgacgagatc gagcgagcga
     6121 tcacgatgcc gccgcgcgac acacgcgcgt acttccgagg gcgttgcctg gcgcagttcc
     6181 cgggcaagat ctcggcggcc tcgtgggact cggtgatctt cgacaccggc ggcgagacgc
     6241 tgcagcgggt gcccatgatg gagccgctgc gaggcaccga ggctcacacg aaggcgttgc
     6301 tggacgcatg ccggacggcg gaagagctcg tcgaccggct gcagggatag cgtcgcccgg
     6361 acgggatagg gacagggagg aggtgaaagc gagatggcga agccgagcgg cggatccgaa
     6421 cagaagaaga agggcacgca gaagcaggac ggcggcgacg acgccggaca ggcgtcggcc
     6481 gaggtgatcg caaagggcga agagctcaag gaagacatcg acgagctctt ggacgagatc
     6541 gatgaggtcc tcgaaaccaa cgccgaagag ttcatccggt cgtacgtgca aaagggcggc
     6601 gagtaatttg tcctgtttgc ggactccccg ttgagcgggt ccggttcagg tgccggtgcg
     6661 gacggtggtg gccggtgcgg gcggtggtgc gggcttcgga ctcgtcaaca aagttgacga
     6721 gtggctgacg acggacgggg gcacaggtcc acggaaccct gcgaaccacc cggcgtcgct
     6781 gcgcgccctg cgatagcctt cggttcctat gagcgagctt ccgatcgact ggaccaaggc
     6841 ggcgttctcg tccgacgcga acccgtcctt caacgacttc gtgcagcgca ccgcgccggt
     6901 cctgctgccg caggtcacgc cgggcgcggg cgggatgccc gagcagccgc acggcaccac
     6961 catcctggcc ctcacctacg ccgacggcgt catcatcgcc ggcgaccggc gcgcgacctc
     7021 gggctattcc atcgccgacc gcaagatcga gaaggtcttc ccggcagacg actattcggc
     7081 cgtggcgatc gccggggctg ccggccccgc gatcgacatg gtcaagctgt tccgcaccga
     7141 gctcgagcac tacgaaaagg tcgagggcga gcgtctgtcg ctggagggca aggccaacaa
     7201 gctggcgtcg atgatcaaga tgaacttgcc gttcgcgatg cagggctttc tggttgtgcc
     7261 gctcttcgcc ggctacgacg agcgccgcca ggccggccgc atcttccgct acgacggcat
     7321 cggcggccgc tacgaggaga gcgactacca cgccgacgga tccggcggca tgcacgcgcg
     7381 cggctcgctg aagggccggt ggcgccgcgg cttgaaccgc gaagacgcgg tgctcaccgc
     7441 ggtggaggcg ctcttcgacg ccgcggagga ggacgtcgcg accggcgggc cggacctgat
     7501 ccgcaacatc tatccggtcg tggccaccgt gaccgccgag ggataccgtc gcgtcgacga
     7561 cgacgaggtg ggccgggccg tggacggcgc gatcgcagac cgccgcgaac gcgaggagcg
     7621 caaccgatga gcccgatgcc gtactacgtc tcgcccgagc agctcatgaa ggaccgggcc
     7681 gactacgccc gcaaaggcat cgcgcgcggc aagtccatca tcgcggtcga gtgcgatgaa
     7741 ggcatcttga tcgtcgccga gaaccccagc gccacgctgc acaaggtcag cgagatctac
     7801 gaccggatcg ccttcgccgc cgtcggcaag tacaacgagt tcgaaaactt ccgaatcgcc
     7861 ggtgtgcgtc tggccgacct gcgcggctac gcctacgacc gcgaggacgt cagcggccgc
     7921 gcgatcgcca acgcctacgc gcagacgctc ggcgcgatct tcaccgagca gatgaagccc
     7981 tacgaggtcg agcttctcgt ggcgcaggtc ggctcctcgc aggagaccga cgagatgtac
     8041 cgggtgatgt acgacggcag cgtgagcgac gagcaaggct tcatggcgat gggcgggcac
     8101 gccgaggcgc tggcgaacga ccttcagcag ggttaccggc aacagatcgg catcggggac
     8161 gcgttcacgc tcgctgctaa ggcgctcgcc gccgccgatt cgggccgaac cctgacgcct
     8221 gcgaacctgg aggtggccgc gctcgagcgg caccggccgc gccgcacgtt ccgccgttac
     8281 tccgacgagg aagtgggcga gctcctggcg cagggttcgt agtgccggcc gggcaggctc
     8341 catcgcatcc gccaccgccg gcgtttcccc ccccgccgct cccggagccc gaccggaacc
     8401 atcccgcccc gttcgccatc ctgctggtgc tgctcgccgt gtcgatcatg gcgagcgtca
     8461 cgctggccgt gcgcgtcgcc gacgcc
//
LOCUS       DATLFO010000219         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_86679, whole genome shotgun sequence.
ACCESSION   DATLFO010000219 DATLFO010000000
VERSION     DATLFO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86679"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VM841_08930"
     CDS             complement(<1..444)
                     /locus_tag="VM841_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HVL90343.1"
                     /translation="MARILLFAAAREAAGRREDRIDAATVGEALKVAGERYGQGFERL
                     AAICSVLRNGEVVARDQWWSAGVGDDDEIAILPPVSGGSDFAMIDVADKDETVREAVA
                     VCTVSGDAGVVARLLDGTVKKGDAVGAAKVAATLAAKRTPELIPLC"
     gene            complement(482..1699)
                     /gene="glp"
                     /locus_tag="VM841_08935"
     CDS             complement(482..1699)
                     /gene="glp"
                     /locus_tag="VM841_08935"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVL90344.1"
                     /translation="MKRLVTPEELRRPLVPYEEAVATILASFAALEPQRTPVAAALGS
                     VAAETVVADLAVPGFDNSAMDGYAIRAADTAGAGDKPVTLRLIDDLPAGSDPTIPIEP
                     GTAATIMTGAAVPPGCDAVVPWEDTEPGDGVVIVKRELDPGRNIRPKGEDLEEGREIV
                     TAGGVLRPVHLGVLASIGRTHVLVHPRPRVAVLSTGDELVPPGGKLMGGQLFESNRTM
                     IAALCEHDGARVVRDGLLPDDPEAIGEWMREAAGGADLIITSGGASVGEHDWIRDILE
                     REGSLSMWRVAIKPGKPVALGRLAGTPVLALPGNPGSAFVGMHVFGLPALRIMAGRDP
                     RPPRVEATLAAPVKGSPARTQFTRVRLDGDAAHPLPLQSSVVLSNLLGADGFAVVPPG
                     GLPEGAAVTIELL"
     gene            complement(1817..2068)
                     /locus_tag="VM841_08940"
     CDS             complement(1817..2068)
                     /locus_tag="VM841_08940"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HVL90345.1"
                     /translation="MNDWMQDVIDSLAQRTGLDPATLTLSSDEQRTILDVARVAAHSS
                     GARINAPLLCYVLGVAAAQGAPLSATASIVHERDTEQAQ"
     gene            2051..>2254
                     /locus_tag="VM841_08945"
     CDS             2051..>2254
                     /locus_tag="VM841_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HVL90346.1"
                     /translation="MHPIVHAGLLLTGGASTRMGRDKATIVAGGASLAERSAAALRAV
                     AAPVLAVGQEASTGCAVVDDPRQG"
BASE COUNT          306 a          826 c          825 g          297 t
ORIGIN      
        1 gcacagcggg atgagctcgg gcgtgcgctt ggccgcgagc gtcgcggcga ccttggccgc
       61 gccgaccgcg tcgcccttct tcaccgtgcc gtcgagcagc cgggccacaa cgccggcatc
      121 gccgctcacc gtgcagaccg cgacggcctc gcggacggtc tcgtccttgt ccgcgacgtc
      181 gatcatcgcg aagtccgatc cgccggagac cggcggcagg atcgcgatct cgtcgtcgtc
      241 gccgacgccc gccgaccacc attggtcgcg cgccacgacc tcgccgtttc ggagcacgga
      301 gcagatcgcg gcgaggcgtt cgaatccctg cccgtagcgc tcgcccgcca ccttgagcgc
      361 ctcgcccacg gtggccgcgt cgatgcggtc ctcgcggcgg ccggccgcct cgcgggcggc
      421 ggcgaacaga aggatgcgtg ccatgacgac aaagcctagc ccgaatccgg gaacggcgcg
      481 gttacagaag ctcgatcgtg actgcggcgc cttccggcag gccgccaggt ggcacgacgg
      541 cgaagccgtc cgcgccgagc aggttggaca gcaccacgct cgactgcagc ggcagcggat
      601 gcgccgcgtc gccgtcgagc cgcacgcgcg tgaactgcgt ccgggccggc gagcctttca
      661 ccggcgcggc gagcgttgcc tccacgcgcg gcgggcgtgg gtcgcggccg gccatgatgc
      721 gaagggccgg caggccgaac acgtgcatgc cgacgaacgc ggatccgggg ttgccgggca
      781 gggccagcac cggcgttccg gcgagccggc cgagcgccac cggcttgccg ggcttgatcg
      841 cgacccgcca catcgacagc gagccttccc gctcgaggat gtcgcggatc cagtcgtgct
      901 ctccgaccga cgcacccccg gaggtgatga tcagatcggc tccgccggcc gcctcgcgca
      961 tccactcccc gatcgcttcg gggtcgtcgg gcaggaggcc atcgcgcacg acgcgcgcgc
     1021 cgtcgtgctc gcacagcgcg gcgatcatgg tccgattcga ctcgaagagc tggccgccca
     1081 tcaacttgcc gccgggcggc acgagctcgt cgccggtcga gagcaccgcc acgcgcggcc
     1141 gcgggtgcac cagcacgtgc gtgcggccga ttgatgccag cacgccgagg tgcacgggcc
     1201 ggagcacccc gcccgcggtc acgatctcgc ggccctcttc caggtcctcg cccttgggcc
     1261 ggatgttgcg tccgggatcg agctcacgct tgacgatcac gaccccgtcg cccggctcgg
     1321 tgtcctccca cggcacgacc gcgtcgcagc ccggtggaac ggccgcgccg gtcatgatcg
     1381 tggcggcggt gccgggctcg atcgggatgg tgggatccga tccggcggga agatcgtcga
     1441 tgaggcggag cgtgacgggc ttgtcgccgg cgccggcggt gtcggcggcg cggatcgcgt
     1501 agccgtccat cgccgagttg tcgaagcccg ggacggccag gtccgccacg accgtctcgg
     1561 ccgcgaccga cccgagcgcc gccgccaccg gcgtgcgctg cggctccaac gcggcgaacg
     1621 acgccaggat cgtcgcgacc gcctcttcgt agggaaccag gggccgccgg agctcttcgg
     1681 gcgtgaccaa tcgcttcatc atcgcaagcc taccgggtcc gctcgagcgc ggtgtccgtc
     1741 cgagcggcgg gcccgcccga gcgccgggcc cgccgcgctc ggggccagag cgcgaggccc
     1801 gagcgcgaag tccgtcctac tgcgcctgct cggtgtcgcg ttcatgcacg atcgacgcgg
     1861 ttgccgacag cggcgcgccc tgggccgcgg ccacaccgag gacgtagcat aggagcggcg
     1921 cgttgatacg cgccccggac gagtgggcgg cgacccgggc gacatcgagg atcgtgcgct
     1981 gctcgtcgga cgacagcgtc agcgtcgccg ggtcgaggcc ggtccgctgc gcgagggagt
     2041 cgatgacgtc ttgcatccaa tcgttcatgc gggcctcctg ctgacgggcg gcgcatccac
     2101 tcggatgggc cgcgacaagg caacgatagt cgcgggcggg gcatcgctgg cggagcgatc
     2161 ggccgcggcg ctgcgcgcag ttgccgcgcc ggtgctcgcc gtcgggcagg aagcgagcac
     2221 cggatgcgcc gtcgtggacg acccccggca gggg
//
LOCUS       DATLFO010000220         5581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_107364, whole genome shotgun sequence.
ACCESSION   DATLFO010000220 DATLFO010000000
VERSION     DATLFO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107364"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /gene="ftsH"
                     /locus_tag="VM841_08950"
     CDS             <1..1888
                     /gene="ftsH"
                     /locus_tag="VM841_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVL90347.1"
                     /translation="LSLALVLIGVWFNMIDDLKPIAQGRQVPISRVFDLAAEKQIKAI
                     ELKDEDATVLVALNSRQGYTFYSRYPSSDSATSQLIEAVQQGGGEIVVDPQDDKGSKR
                     FLVQFLLPLLILAALFSFFFLLITGQSGGANEFFAFSKSLAKITKRKKGTKGEVTFDD
                     VAGNDEGLIELREAVDYLTNPERFASLGAKAPKGILLAGPPGTGKTLLAKATAGEAAV
                     PFFSMAGSEFVEALVGVGAARVRDLFRKARANAPCLIFIDELDAAGRQRGAGVGQGND
                     EREQTLNQMLAEMDGFDVSAGVVVMGATNRPDILDPALLRPGRFDRQVIIDVPDVTGR
                     FEILKIHARNRPMAPDADLMVIAKQCPGFTGAELANIMNEAALLAVRNDHQGITNEDL
                     DEAVERTLAGPARKGHLLSAQEKLLVAYHEAGHAIVARAVGQQVGIMKLSIVARGRQL
                     GHATVYQQADKLVVLKSEIEAELTVIMGGLAAENIVFGEISTGNTGDLEKATDLARKM
                     VATWGMTHEVGRVTIQKAGAQYLGRDAASLMSVSQNVLESVDREIQNFIEEAEHRAEK
                     IVRANKAVMDEITEVLLERETLSHEDVEPYLSRVKPVQVGEGKGMRTSVRASSTAQRR
                     GRSR"
     gene            1991..3107
                     /gene="prfB"
                     /locus_tag="VM841_08955"
     CDS             join(1991..2059,2061..3107)
                     /gene="prfB"
                     /locus_tag="VM841_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759338.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HVL90348.1"
                     /translation="MPTDFSAELEALNDAVAGARAYLDVDGKRSKAESLREQTAAPDL
                     WDDQDRARALMGELARLDDDLALYDELSGAVADLQVLNELAQAEGDESAAGEVAEGVA
                     TLQRRIKELDERTLLGREYDDNDAIVAVHAGAGGTESQDWAEMLMRMYLRWAERRKIA
                     VEILEVSAGDEAGIKSATFTAKGRFAFGLLSAEGGVHRLVRISPFDTNKRRHTSFASV
                     DVVPMIDEDVQVDINPDDLRVDVYRSSGAGGQHVNTTDSAVRITHLPTGIVVSCQNER
                     SQLQNRATAMRVLQARLAELMRQEHVAKIDELRGERREINFGSQIRSYVLHPYQMVKD
                     HRTSFETSNTTGVLDGEIDEIIRSTLEWRRAGGETAS"
     gene            complement(3110..3451)
                     /locus_tag="VM841_08960"
     CDS             complement(3110..3451)
                     /locus_tag="VM841_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90349.1"
                     /translation="MTFDRRAFEIAARHAVATERPPLDRLTGIGEIRRMLPLLEEAAI
                     RDARAQRITWATIAFWMGVSHQAVIKRWRTRLYGDAYDPMSSRIRPDRLPPRGPAPTS
                     PMKFWTVPAGE"
     gene            3930..4766
                     /locus_tag="VM841_08965"
     CDS             3930..4766
                     /locus_tag="VM841_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVL90350.1"
                     /translation="MPEQTRDAGRLEEIARDLRVDIIRMLVEAKSGHPGGSLSEIDIL
                     TCLFFGGILHYKPDQPDWPDRDRFLLSKGHCTPGLYTTMARAGYFPAEELMTFRKLGS
                     RLQGHPDRTALPGIEVSAGSLGQGLSVAVGMALAARMDGHAWRTFCMMGDGEQDAGQV
                     WEAAMAAGDYGLGNLVGIVDCNQVQQTGLTKEIMTLEPIVDRYRSFGWHVIEFDGHDI
                     PAILGAFDDALSVEDKPAAMIARTVKGKGVSWMELNYNWHGKAPSVEEGERAIAEILG
                     GN"
     gene            4769..>5581
                     /locus_tag="VM841_08970"
     CDS             4769..>5581
                     /locus_tag="VM841_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVL90351.1"
                     /translation="MASMATRQAFGDAIVEIGSDPRVVVIDGDLKNSTYTEDFLAKYP
                     ERFVECGIAESNMVGIAAGLALSGKVPWACSFSAFITGRSEVIRVSVCYNRANVRLVG
                     THVGIGIGDDGATQMALQDIAQMRALSNMTVIQPCDRIETIQAVHALLEHDGPVFLRL
                     MRQATEDVHGDDYRFTLGKADVLRDGSDVALIATGALVQESLKAADALGEDGIAARVI
                     NIHTIKPLDAEAIARAAADCGAIVTAEDHAIEGGLGGAVAEAALDRVVLSGDD"
BASE COUNT          933 a         1876 c         1873 g          899 t
ORIGIN      
        1 gcttagcttg gcgcttgttc tgatcggcgt ctggttcaac atgatcgacg acctcaagcc
       61 gatcgcccag ggccgccagg ttccgatctc ccgggtgttc gatctagccg ccgagaagca
      121 gatcaaggcc atcgagctca aggacgagga cgccaccgtc ctcgtcgcgc tcaactctcg
      181 ccagggctac accttctact cgcgctaccc ctcgtcggac tcggccacct cgcagctgat
      241 cgaggccgtg cagcagggcg gcggcgagat cgtcgtcgac ccgcaggacg acaagggctc
      301 caagcgcttc ctggtgcagt tcctcctgcc gctgctgatc ctggccgcgc tgttctcgtt
      361 cttcttcctg ctcatcacgg gacagtcggg cggcgcgaac gagttcttcg ccttctcgaa
      421 gtcgctcgcc aagatcacca agcgcaagaa gggcaccaag ggtgaagtta ccttcgacga
      481 cgtcgccgga aacgacgaag gcctgatcga gcttcgcgaa gccgtcgact atctgacgaa
      541 ccccgagcgg ttcgcctcgc tgggcgccaa ggcgccgaag ggcatcctgc tcgccggccc
      601 gccgggaacc ggcaagacgc tgctcgccaa ggccacggcc ggcgaagcgg ccgtgccgtt
      661 cttttcgatg gccggttccg agttcgtcga ggcgctggtc ggtgtcggtg ccgctcgtgt
      721 gcgtgacctg ttccgcaagg cccgcgcgaa cgcgccctgc ctgatcttca tcgacgagct
      781 cgacgccgcc ggccgtcagc gtggtgccgg ggtcggtcaa ggaaatgacg agcgcgagca
      841 gacgctgaac cagatgctcg ccgagatgga cggtttcgac gtgtcggccg gtgtcgtggt
      901 catgggcgcc accaaccgtc ccgacattct cgaccccgcg cttctgcgtc cgggccgttt
      961 cgaccgtcag gtcatcatcg acgtgccgga cgtcaccggc cgcttcgaga tcctcaagat
     1021 tcacgcgcgt aaccgtccca tggcgcccga cgcggacctg atggtcatcg ccaagcagtg
     1081 cccgggcttc accggcgccg agctggccaa catcatgaac gaggccgcgc tgctggccgt
     1141 gcgtaacgac caccagggca tcaccaacga ggacctcgac gaagcggtcg agcggacgct
     1201 cgccggtccg gctcgtaagg ggcatctgct ctccgcgcag gagaagcttt tggttgccta
     1261 ccacgaagcg gggcacgcga tcgtggcgcg cgccgtcggc cagcaggtcg gcatcatgaa
     1321 gctgtcgatc gtcgcccgtg gccgtcagct cggtcacgcg acggtctacc agcaggccga
     1381 caagctggtc gtgctcaaga gcgagatcga agccgagctc accgtgatca tgggtggtct
     1441 ggccgccgaa aacatcgtct tcggcgagat ctcgaccggc aacaccggcg acttggagaa
     1501 ggccacggac ttggcgcgaa agatggtcgc cacgtggggt atgacccacg aggtcggccg
     1561 cgtcacgatc cagaaggccg gtgcccagta tctgggccgc gacgccgcct cgctgatgag
     1621 cgtgtcgcag aacgtgctcg agtcggtgga ccgcgagatc cagaacttca tcgaagaggc
     1681 cgagcaccgt gccgagaaga tcgtgcgcgc caacaaggcc gtgatggacg agatcaccga
     1741 ggttctgctc gagcgcgaga cgctcagcca cgaagacgtc gagccctacc tttcccgcgt
     1801 caagccggtc caggtcggcg aaggcaaggg catgcgcacg tcggtgcggg cctcgtccac
     1861 ggctcagcgc cgcggccgca gccgctagcg gcgatcgcat cgcttcgaac gcgccgtccc
     1921 gtgtgggcgg cgcgttcgcg ttcggcggcg cgttcgcgtt cggcggcgcg ttcgctatta
     1981 tctgcgcgtc atgcccaccg atttctctgc ggagctcgag gccctgaacg acgccgtcgc
     2041 cggcgccagg gcctatcttt gacgtagacg ggaaacgctc caaggccgaa tcgctccgcg
     2101 agcaaaccgc cgcgcccgac ctgtgggacg accaggatcg ggcccgtgcc ctgatgggtg
     2161 agctggctcg cttggacgac gatctggcgc tctacgacga gctgagcggc gcggtggcgg
     2221 acctgcaagt gctcaacgag ctggcccaag cggagggcga tgaatcggcc gcgggtgagg
     2281 tcgccgaagg cgtggcgacg ctgcaacgcc gaatcaaaga gctggacgag cgcacgctcc
     2341 tgggccgcga gtacgacgac aacgatgcga tcgtggccgt gcacgccggc gccggcggca
     2401 ccgaaagcca ggactgggcc gagatgctga tgcgcatgta cctgcgctgg gcggagcggc
     2461 gaaagatcgc ggtggagatc ctcgaggtgt cggccggcga tgaggccggg atcaagagcg
     2521 cgacgttcac ggcgaagggg cggttcgcgt tcgggctgct gtcggccgaa ggcggcgtgc
     2581 accggctggt gcgcatctcg ccgttcgaca cgaacaagcg gcggcacacc tcgttcgcgt
     2641 cggtggacgt ggtgccgatg atcgacgagg acgtgcaggt cgacataaac cccgatgatc
     2701 tgcgcgtgga cgtgtatcgg tcgtcgggcg cgggtgggca gcacgtcaac accaccgaca
     2761 gcgccgtgcg gatcacgcac ctgccgaccg gcatcgttgt gtcgtgtcag aacgagcgct
     2821 cgcagcttca gaaccgcgcg accgcgatgc gtgtgttgca ggcgcgtctg gccgagctga
     2881 tgcgccaaga gcacgtcgcc aagatcgacg agctgcgcgg cgagcggcgc gagatcaact
     2941 tcggatccca gatccggtcc tacgtccttc acccgtatca gatggtgaag gaccatagga
     3001 cgagcttcga gacgtccaac accactggtg tcctcgacgg agagatcgac gagatcatcc
     3061 gctcgacgct ggagtggcgc cgcgccggcg gcgagaccgc cagctagccc tattctcccg
     3121 cgggcaccgt ccagaacttc atgggtgacg tgggtgccgg tccccgcggc ggcagccggt
     3181 cgggccggat tcgcgaggac atcggatcgt acgcgtcgcc gtacaggcgc gtgcgccatc
     3241 gcttgatcac cgcctgatgg ctcactccca tccagaaggc gatcgtcgcc caggtgatcc
     3301 gctgcgcccg agcgtcccgt atcgcggctt cctccagcag cggcagcatg cgccggatct
     3361 cgccgatgcc cgtgagccga tccagcggcg gccgttctgt cgctaccgca tgtcgggccg
     3421 cgatctcgaa tgctcgccgg tcgaatgtca tgagcaagga tcttgagcgg tcatcacctt
     3481 cgtttccagc gatcgcgccc gggtcgcgcc cgatcggaac attgtccggg gccgccacga
     3541 gcttctccag ccccaagggt tccccgaacc ctgtgcaacc aagttgcaac gagagccacg
     3601 gtcgcgcgct gccgccggct ctcggctctc ggctcccggc gcccggctct cggctctcgg
     3661 ctctcggcgc ccggcgcccg gcgcccggct ccgggccggg tcccaccctt ccggcgtggc
     3721 ttgcaacttg gttgcacagg gtgccggcgc ccttccgccc ggcccgcaac ttggttgcac
     3781 agggtgctca gaggtatccc ggtcctgggt ctgtgggtgg ggtccgtcgc ggcggcgcgg
     3841 atgggcggtt cgcggccgac ccggcgggga acgtagaatt caacgcggcc ttcccgcagc
     3901 cgatacgacc gatccgaagg agttttgtcg tgcccgagca gacgcgcgac gccggccgcc
     3961 tggaagagat cgcacgcgac cttcgcgtcg acatcatccg tatgctggtg gaagccaagt
     4021 ccggccatcc cggcggctcg ctgtccgaga tcgacatctt gacctgcttg tttttcggcg
     4081 ggatcctcca ctacaaaccc gatcagccgg actggccgga tcgcgaccgc ttcctgctct
     4141 cgaaggggca ctgcaccccg gggctgtaca cgaccatggc ccgcgccggc tactttccag
     4201 ccgaagagct catgaccttc cgcaaactcg gctcgcggct gcagggccac ccggaccgca
     4261 cggcgctgcc gggcatcgag gtcagtgccg gctcgctcgg tcagggtctg agcgtcgcgg
     4321 tcggcatggc gctggcggcc cgcatggacg gacacgcctg gcgaaccttc tgcatgatgg
     4381 gcgacggcga gcaggacgcc ggccaggtct gggaagccgc gatggctgcg ggtgactacg
     4441 ggctcggcaa tctcgtcggc atcgttgact gcaaccaggt gcagcagacc ggcttgacga
     4501 aagagatcat gacgctcgag ccgatcgtgg accgctatcg atcgttcggc tggcacgtga
     4561 tcgagttcga cggccacgac atccccgcca tcctcggcgc cttcgacgac gctctctcgg
     4621 tcgaggacaa gcccgccgcc atgatcgccc gcacggtcaa gggcaagggc gtgtcctgga
     4681 tggagctgaa ctacaactgg cacggcaagg cgcccagcgt cgaagagggc gagcgcgcga
     4741 tcgccgagat cctcggaggg aactgatcat ggcatccatg gcgactcggc aagcattcgg
     4801 cgatgcaatc gtcgagatcg gctccgatcc ccgcgtcgtc gtcatcgacg gcgacctcaa
     4861 gaactccacc tacaccgaag actttctcgc gaagtatccg gagcgcttcg tcgagtgcgg
     4921 catcgcggaa tcgaacatgg tcggcatcgc ggccgggctc gcgttgtcgg gcaaggttcc
     4981 gtgggcgtgc tcgttcagcg cgttcatcac cggccgctcc gaagtcatcc gcgtcagcgt
     5041 ctgctacaac cgcgccaacg tccgcctcgt gggtacgcac gtcggcatcg gcatcggcga
     5101 cgacggcgcg acgcagatgg cgctgcagga catcgcacag atgcgcgcgc tgtcgaacat
     5161 gaccgtgatc cagccgtgcg accgcatcga gacgatccag gccgtgcacg cgctgctcga
     5221 gcacgacggt ccggtgttcc tgcggctcat gcgtcaggcg accgaggacg tgcacggcga
     5281 cgactatcgg ttcacgctcg gcaaggccga tgtgctgcgc gacggaagcg acgtcgcgct
     5341 gatcgcgacc ggcgcgctgg tgcaggagtc cctcaaggcc gccgacgcgc tcggggagga
     5401 cggcatcgcc gcccgcgtga tcaacatcca caccatcaag ccgctcgacg ccgaagctat
     5461 cgcacgtgcg gcggcggact gcggcgcgat cgtcaccgct gaggaccacg cgatcgaggg
     5521 cggcctcggc ggagccgtgg ccgaggccgc cctcgatcgc gtggtcctca gcggtgacga
     5581 t
//
LOCUS       DATLFO010000221         4671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_94617, whole genome shotgun sequence.
ACCESSION   DATLFO010000221 DATLFO010000000
VERSION     DATLFO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94617"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VM841_08975"
     CDS             <1..871
                     /locus_tag="VM841_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90352.1"
                     /translation="VVSAGNLGPGAGTISKPADDPLVITVGAIDDRESPGTSDDRLPS
                     FSARGPAFPGTPDEHVKPDVVAPGGNVISLRVGGSFVEQHAPGGLPHQSYRRGSGTSM
                     AAGVTSGAIALMLQANPAWRGNPDRVKFALAATARKVAERFVYAIGAGLIDVYAATRS
                     APAGVANGWVTASSDGSGSLDGSRADVLVSRVCAPFEQSLNPTCDHVNGENTGQDRAF
                     DSEQFRGTWNEASWYRSQWVTGLTNPQWYGSSWYGSSWYGSSWYGSSWYGSTDGTFYG
                     VSVAGSSWYGAWN"
     gene            871..3621
                     /locus_tag="VM841_08980"
     CDS             871..3621
                     /locus_tag="VM841_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVL90353.1"
                     /translation="MGPGGGAAPRTRGRVAAFVVVLAAAAAVVTTWLAWTGADPPGRA
                     SWPALVMLGGVLIAAEFLFVRFRYGGEINALNLFEAALAPLVWAYPPAAVVGVVAGSQ
                     IIGNLLRRNAPIKSAFNVVQWSLAAALASATVHAIARDAGVTAAGLLAMVGALIVVTV
                     VNQSCFSVVLALANKERLGVVLRGLSGAIVPGWVVGVALNSAVGVLFVLAFEAHPASV
                     VLFPVPLIVLHLAYRGYAGARSDRMRLAGLHSAATVLAESFEPESAIEPFLRELARSF
                     EARGAAIVLRTNGSFTARRIGPGDGPMTVVAGAESTAAPESLLLGRPAGRVTVRDGGP
                     LAAALAAAGFRDCLSAPLQQDAGSIGALMVFDQVGLEGFEAGEVAVLEALARETAGVL
                     AKGRLLSAIVEERQRLDEIVSATSDGIFTMDEDGCVRKWNPAMERITGISAADATGRA
                     GVFAALVARSADGEPVDFGRWTRRDLPREFLISVAGSGPRRLSCSFSETGGEARARRL
                     VGVVRDATAEEEMQSLRREFDQLTRMQRQQRQVVEQLQRAVVPPRPRVDGVQLGLRYQ
                     ASETQSPTGGDLFDWQVLPDGEMHVVVVDVLGHGVAATNDAVSVIHVLRTLAVEGCPI
                     EEMIGRADALLRSLNPDLVATVLIARIDPVTGHTTLAGGGHPPALHVRATGEVRQIPA
                     PGGAIGWPHAGSIGVAETTLGPGDSLILYTDGLIEGTKDVLHGEESLMEHARVLAPLP
                     ADEIASGLIERSLEPVERRDDSLALVVRVSPPVERFSIDVEPGPIGAHRARIEAATWL
                     KAVGVSDGTLPDVELALGELLANAAVIARTRVRVGLVVEPATVAVEVEDDGPGDPELE
                     TKGLSPSSADVDAGRGLFIVRAVSSSLSFLSTSSGTVVRAVLGRDLSTSASSRTRESR
                     HA"
     gene            complement(3618..3959)
                     /locus_tag="VM841_08985"
     CDS             complement(3618..3959)
                     /locus_tag="VM841_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL90354.1"
                     /translation="MAIENELRIESLDAPRHLKVVGDLDLASAPLLESSLEPLLKEAG
                     DLTLDASGMTFIDSSGIRALIRASLRLGDARLIILKPSAQLKRLFTITGIQQGERIDL
                     RNDDCCSHRAR"
     gene            4095..>4671
                     /locus_tag="VM841_08990"
     CDS             4095..>4671
                     /locus_tag="VM841_08990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HVL90355.1"
                     /translation="MKFGITGVNAGRRTQASRAAMLARMAEEAGFESLWAVEHVVVPT
                     GYRSQYPYSEDGKMPGREDIAIADPLVWLSYVAAVTESIMLATGIVILPQRNPVLLAK
                     ECASLDVLSNGRLILGIGIGWLEEEFRALGVPFEDRAARTDEYAAAMRVLWEDDEPTF
                     DGRFASFEKALSYPKPARSIPLVVGGHSEAAA"
BASE COUNT          663 a         1552 c         1660 g          796 t
ORIGIN      
        1 ggtggtgtcg gcgggcaacc tcggtcctgg ggccggcacg atctccaaac ccgccgacga
       61 tccgctcgtg atcaccgtcg gcgcgatcga cgaccgcgag tctcccggga cgtccgacga
      121 ccggcttccg agcttctcgg cgcgcgggcc cgccttcccc ggaacgcccg acgagcacgt
      181 caagcctgac gtcgtcgcgc ccggcggcaa cgtgatctcg cttcgcgtcg gcggcagctt
      241 cgtcgagcag cacgcgcccg gtgggttgcc gcaccagtcc tatcgccgcg gcagcggaac
      301 ctcgatggcg gccggcgtca cctcgggcgc gatcgccctg atgctgcagg ccaacccggc
      361 ctggcgcggc aacccggatc gggtcaagtt cgcgctcgcg gccacggcgc gcaaggtcgc
      421 cgagcgcttc gtatatgcga tcggcgcggg ccttatcgac gtgtacgccg cgacgcgctc
      481 ggctccggcg ggggtcgcga acggctgggt gaccgcgtcg tcggacggat ccggctcgct
      541 cgacggcagc cgggccgacg ttctggtctc gcgcgtgtgc gcgccattcg agcagtcgct
      601 gaacccgacg tgcgatcacg tcaacgggga gaacaccggg caggatcgtg cgttcgactc
      661 tgagcagttc cgcggcacat ggaacgaggc aagctggtac cgcagccaat gggtgaccgg
      721 cctgacgaac ccgcagtggt acggatcctc gtggtacgga tcctcgtggt acggatcgtc
      781 gtggtacgga tcctcgtggt acggatccac cgacggcact ttctacggcg tgtccgttgc
      841 gggctcgtcc tggtacggcg cgtggaactg atgggacccg gtggcggcgc ggcgcctcgg
      901 actcgaggcc gcgtcgccgc gttcgtggtc gtcctcgcgg ccgcggccgc cgtagtcacc
      961 acctggctgg cctggacggg agcagatcct cccggccgcg cttcatggcc ggcgctcgtc
     1021 atgctcggcg gcgtattgat cgccgccgag tttctgtttg ttcggtttcg ctacggcggc
     1081 gagatcaacg cgctgaacct gttcgaggcc gcccttgcgc cgctcgtgtg ggcctacccg
     1141 cccgccgcgg tggtcggcgt cgtggcgggc tcccagatca tcggcaatct gcttcgccgg
     1201 aacgcgccga tcaagtccgc gttcaacgtc gttcagtgga gcctcgccgc cgccctggcc
     1261 tcggccaccg tgcacgcgat cgctcgcgat gcgggcgtca ccgccgccgg cctgctcgcg
     1321 atggtcggcg cgctgattgt ggtgaccgtg gtgaaccagt cctgcttctc ggtcgtgctg
     1381 gcgctcgcga acaaggaacg gctcggcgtg gttctccgcg ggctgtccgg cgcgatcgtg
     1441 cccggatggg tggtcggggt cgcgctcaac tccgcggtcg gcgtgctgtt cgtgctggcg
     1501 ttcgaggcgc atcccgcgtc ggtcgtgctg ttcccggttc cgctgatcgt tctgcacctc
     1561 gcctatcgcg gctacgcggg cgcgcgcagc gaccggatgc gtctggccgg tctgcactcg
     1621 gccgcaaccg tgctcgccga atcgttcgag ccggaaagcg cgatcgagcc gttcctgcgg
     1681 gagctcgccc gaagcttcga ggcgcgcggg gctgcgatcg tgctgcgcac caacggcagc
     1741 ttcacggcgc ggcgcatcgg gccgggagac ggcccgatga cggtcgtcgc cggagcggaa
     1801 tccacggccg cgccggagtc gctcttgctc gggcgcccgg cggggcgcgt cacggtgcgg
     1861 gacggcgggc cgctcgcggc tgcgctggcc gcggccggat tccgcgattg cctgtccgcg
     1921 cccctgcagc aggacgccgg gtcgatcggc gcgttgatgg tcttcgacca ggtcgggctg
     1981 gagggcttcg aagcgggcga ggtcgccgtg ctcgaggcgc tcgcgcgcga aaccgccggc
     2041 gtgctcgcca aagggcggct cctgtcggcg atcgttgagg agcgtcagcg actggacgaa
     2101 atcgtgagcg caacgtcgga cggcatcttc acgatggacg aggacggttg tgtccggaag
     2161 tggaaccccg caatggaacg catcaccggc atctcggccg cggacgcgac cgggcgggcg
     2221 ggcgtgttcg ccgccctcgt ggcccgctcc gccgacggtg agccggtgga tttcggccgc
     2281 tggacccggc gagaccttcc acgtgagttc ttgatcagcg tggcgggaag cgggccgcgt
     2341 cgtctctcct gctcgttcag cgaaaccggc ggcgaggccc gggcccgccg gctcgtgggc
     2401 gtggtccggg atgcgaccgc ggaggaagag atgcagtcgc tccgacgcga gttcgaccag
     2461 ctcacccgga tgcagcggca gcagcgtcag gtcgtcgagc agctgcaacg agccgtcgtc
     2521 cctccgcggc cccgcgtgga cggtgtgcag ctgggtcttc gctaccaggc gtcggaaacg
     2581 cagtcaccca ccggcgggga tctgttcgac tggcaggttc tgcccgacgg tgagatgcac
     2641 gtggtcgtcg tggacgttct cggtcacggt gttgccgcca ccaacgatgc ggtcagtgtc
     2701 attcacgtgc ttcgcacgct cgcggtcgaa ggctgcccga tcgaggagat gatcggccgc
     2761 gccgacgccc ttcttcgcag cctgaacccc gatctggtcg ccacggtgct catcgcgcgg
     2821 atcgaccccg tgacggggca tacgacgctg gccggaggag ggcatccgcc tgcattgcac
     2881 gttcgcgcga cgggcgaggt tcgccagatt ccggcacccg gtggcgcgat cggatggccg
     2941 catgccggca gcatcggtgt ggccgagacc accctgggtc ccggtgactc gctcatcctc
     3001 tacactgatg gccttatcga gggcacgaag gacgtcctgc acggcgagga atctttgatg
     3061 gagcacgctc gcgtgctggc cccccttccc gcggacgaga tcgcatccgg cctgatcgag
     3121 cgatcgctcg aaccggtgga gcgtcgcgac gactcgctcg cgctcgtcgt ccgcgtgtcc
     3181 ccgccggtcg agcgcttctc gatcgacgtt gagcccggac cgattggcgc ccaccgcgcg
     3241 cgcatcgagg ccgccacatg gctcaaggca gtcggcgtgt cggatgggac gctgccggat
     3301 gtggagctgg cgctcgggga gctgctcgca aacgcggccg tgatcgcacg gacacgcgtg
     3361 cgagtcgggc tggtggtcga gcccgccacc gttgcggtcg aggtcgagga cgacgggccc
     3421 ggcgatcccg agctcgagac gaagggtttg agcccgtcga gtgcggatgt cgatgccgga
     3481 cggggcctgt tcatcgttcg ggccgtgtcc tcatcgctgt cgtttctgtc cacctcgagt
     3541 ggcaccgtgg tgcgcgccgt gctgggacgt gacctaagca cgtcggcatc gagccgcact
     3601 cgggaatcgc ggcacgccta gcgtgcgcgg tggctgcagc aatcgtcgtt tctgaggtcg
     3661 atgcgctcgc cctgctggat gccggtgatc gtgaacaggc gcttgagctg ggccgacggt
     3721 ttcaggatga tcagacgcgc gtcacccaaa cgcaggctcg cgcggatcaa cgcgcggatc
     3781 ccggacgaat caatgaacgt catgccggag gcgtcgagcg taagatcgcc ggcctctttg
     3841 agcagcggct ccaacgagga ctcgagcaag ggagcggacg ccaagtcgag atctcccacc
     3901 accttcaggt gacggggagc gtccagcgat tcaattcgta actcgttctc gattgccaag
     3961 gaaaaggccc ccctccggac cggcgcgcaa tcttttcagg atgcggggtt cctggcaagc
     4021 gagccagacg cgcattgccc gtgagttgtc gcaattgccc ccatgtcgcc gaaggaagaa
     4081 ttagactctg cggcgtgaag ttcgggatca caggcgtgaa cgcgggccgg cggacgcagg
     4141 cgagccgcgc ggcgatgctt gcgcgcatgg ccgaggaagc cggcttcgag tcactctggg
     4201 cggtcgagca cgtcgtcgta ccgacaggat accgatcgca gtatccctac tccgaggacg
     4261 gcaagatgcc gggccgcgag gatattgcga tcgcggatcc tttggtttgg cttagctacg
     4321 tcgcggcggt tacggaaagc atcatgctcg cgaccggcat cgtgatcctg ccgcaacgga
     4381 atccggtcct tctcgcgaag gaatgcgcgt cgctcgacgt tctttcgaac ggccgactga
     4441 tcttggggat cggcatcggc tggctcgagg aagagtttcg cgcgctcggt gtcccgttcg
     4501 aggatcgcgc cgcccgcacc gacgagtatg ccgcggcaat gcgcgttctg tgggaagacg
     4561 acgagcccac cttcgacgga cgcttcgcct cgttcgagaa ggcgctcagc tatcccaagc
     4621 cggcgcgcag catcccgctc gtcgtcggcg gccactccga ggcggcggcg c
//
LOCUS       DATLFO010000222         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_133894, whole genome shotgun sequence.
ACCESSION   DATLFO010000222 DATLFO010000000
VERSION     DATLFO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133894"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="VM841_08995"
     CDS             <1..1927
                     /locus_tag="VM841_08995"
                     /inference="COORDINATES: protein motif:HMM:NF037006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVL90356.1"
                     /translation="PKAVVSSLVCDNVLDGNTDDPAEVFAERAPSYSSFPGGSRFPGN
                     GECVEAGLRFTPAGLTLTEGGPARTYLARLTLRPAANVTVMPSGGSQASVSPQTLTFT
                     PSNWYAGQIVTVAAVDDALVEGTTIHPVAHTASSSDAAYAGVVSEMPLTIKDNDAGTL
                     VLIESDGSTEVAEGGATDTFTVSLTAPPAAPATISFVPDPQITIVPTAVTFTAQNFAT
                     PQTITVTAVDDAVRENDHSGSIEGSSSDPLFGSVPSVLASIKDNDAPAAPAIAFPSEG
                     QILTATDVTVAGTGEPGATVEIFEGMASMGTALVDANGMWSAQIAFGADMTHVITAAQ
                     TGTDGLTGGASAARSFSIDMTAPAAPTITGPADGSSFASGNVTVTGTAEPNATVSVYG
                     NGGLKATTTASGSGAWSVETGFVAGSHAITATARDGAGHESGPSLPVNITVALLTPTI
                     VDPAQGSYAKSPVTVRGTADGSHAFVYLYDFGVLKAVVPVTSGVPGTWQTQVAFDSGV
                     RRLTAISRSAANTISPVSAVRTFTADNTAPTAVVHRPQFYTVFGIVTDGTIRGTAREG
                     EVWDSGVARVDLIYLDASGAQVAAAQATCANCPGSFVSWSHKPDLLPGFYTVQARATD
                     RVGNVSAAGTMAFLLVR"
     gene            complement(1990..>2241)
                     /locus_tag="VM841_09000"
     CDS             complement(1990..>2241)
                     /locus_tag="VM841_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HVL90357.1"
                     /translation="DVDESNESSLLVHDMHREDPALAFMLAHIARGPHEPTPIGIFRS
                     VERTVYGDAMTAQIGAAREKLGEGDMAKLLASGDTWTVN"
BASE COUNT          333 a          814 c          760 g          334 t
ORIGIN      
        1 gcccaaggcc gtagtctcca gcctggtctg cgacaacgtg ctggacggaa acaccgacga
       61 cccggcagag gttttcgccg agcgggcgcc gtcctacagc tcgttccccg gcggatcgcg
      121 cttcccgggc aacggcgaat gcgtcgaggc gggcctgcgc ttcacgccgg ccggtctcac
      181 gctcaccgaa ggcggaccgg ctcgcaccta cctggcccgc ctgacactgc gcccggccgc
      241 gaacgtaacg gtcatgccct cgggcggatc ccaggcgtcg gtcagcccgc agacgctcac
      301 cttcactccg tcgaactggt acgcggggca gatcgtgacc gtggcggccg tcgacgacgc
      361 gctggtcgaa gggaccacca tccatccggt ggcccacacg gcatcctcgt ccgacgcggc
      421 ctacgcgggc gtggtcagcg agatgccgct cacgatcaag gacaacgacg cgggcacgct
      481 ggtgctgatc gagtcggacg gctcgaccga ggtcgccgaa ggcggcgcga ccgacacctt
      541 caccgtgtcg ctgaccgctc ctcccgcggc gcccgcgacg atcagcttcg tgccggaccc
      601 gcagatcacg atcgtgccca ccgcggtcac cttcaccgcg cagaacttcg ccacgccgca
      661 gacgatcacg gtgaccgccg tggacgacgc ggtgcgcgag aacgaccaca gcggctcgat
      721 cgagggctcc tcctcggacc cgctcttcgg gtccgtcccg tcggtgctgg cctcgatcaa
      781 ggacaacgac gcgccggccg cgccggcgat cgccttcccg tcggaaggac agatcctgac
      841 cgcgaccgat gtgaccgtcg ccggcaccgg cgagcccggc gccacggtcg agatcttcga
      901 gggcatggcg tcgatgggca cggccctggt tgacgcaaac ggcatgtggt cggcgcagat
      961 cgccttcggc gccgacatga cccacgtcat caccgcagcg cagaccggaa ccgacggtct
     1021 caccggcggc gcgtcggcgg cgcgttcgtt ctcgatcgat atgaccgcgc cggccgcgcc
     1081 caccatcacc ggaccggccg acggatcgtc gttcgcgtcg ggcaacgtga ccgtgaccgg
     1141 aacggccgag ccgaacgcga ccgtgtccgt ctacggcaac ggcggtctca aggcgacgac
     1201 gactgcgtcg ggatccggtg cgtggtcggt ggagaccggc ttcgtcgccg gctcgcacgc
     1261 gatcaccgcg accgctcgtg acggcgccgg ccacgagtcc ggcccgagcc tgccggtgaa
     1321 catcaccgtg gcgctgctca cgcccacgat cgtcgatccg gcccagggct cctacgccaa
     1381 gtcgccggtg acggtccgcg gcacggccga cggctcgcac gccttcgtct acctctacga
     1441 cttcggggtt ctgaaggcgg tcgtgccggt cacgagcggc gtgccgggaa cctggcagac
     1501 gcaggtcgcc ttcgacagcg gcgtgcgccg cctcaccgcg atcagccgct ccgcggcgaa
     1561 tacgatcagc ccggtatcgg cggtccgcac gttcaccgcg gacaacaccg ccccgactgc
     1621 ggtcgtgcac cggccgcagt tctataccgt gttcggtatc gtgaccgacg gcacgatccg
     1681 cggcaccgcc cgcgagggcg aggtttggga ttcgggcgtg gcgcgcgtgg acctgatcta
     1741 tctcgacgcg agcggcgctc aggtcgcggc cgcgcaggcc acgtgcgcga actgtcccgg
     1801 ctcgttcgtg tcgtggtcgc acaagccgga cctgctgccg ggcttctaca cggtgcaggc
     1861 gcgcgcgacc gaccgggtcg gcaacgtctc ggccgccggc acgatggcgt tcctgctcgt
     1921 gcgatagccg caacggaaac gaacgagagg ccggcgtccg ccggcctctc gtctgttcgg
     1981 gatcgctggt tagttgacgg tccaggtgtc gcctgaggcg agcagcttgg ccatgtcgcc
     2041 ttcgccgagc ttttcgcgtg cggcgccgat ctgcgcggtc atcgcgtcgc cgtagacggt
     2101 acgctcgacc gagcggaaga tgccgatcgg cgtcggctcg tgcggcccgc gggcgatatg
     2161 ggcgagcatg aacgccagcg ccgggtcctc gcggtgcatg tcgtgcacca gcaggctcga
     2221 ctcgttcgac tcgtccacgt c
//
LOCUS       DATLFO010000223         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_58813, whole genome shotgun sequence.
ACCESSION   DATLFO010000223 DATLFO010000000
VERSION     DATLFO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58813"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /gene="rplB"
                     /locus_tag="VM841_09005"
     CDS             <1..541
                     /gene="rplB"
                     /locus_tag="VM841_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HVL90358.1"
                     /translation="YADGEKRYILQPAGVKVGDTVVAGDNVDILPGNCLPLRSIPLGT
                     TIHNVELRPGKGGQLARSAGSSVQLVAREGEYASVRLPSGEIRKINVECVATVGQVGN
                     LDHENVSIGKAGRSRWLGKRPHVRGVAMNPVDHPLGGGEGKTSGGRHPVTPWGQPTKG
                     FKTRRRKGTDRFIVQRRTK"
     gene            542..844
                     /gene="rpsS"
                     /locus_tag="VM841_09010"
     CDS             542..844
                     /gene="rpsS"
                     /locus_tag="VM841_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HVL90359.1"
                     /translation="MSRSLKKGPFVDTPLLEKLEAMNRANEKKVIKTWSRRSTVVPEM
                     VGHTMAVHNGKKFIPVYVTENMVGHKLGEFAPTRTFKGHTTKTEKAATAAPAGGKA"
     gene            854..1300
                     /gene="rplV"
                     /locus_tag="VM841_09015"
     CDS             854..1300
                     /gene="rplV"
                     /locus_tag="VM841_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HVL90360.1"
                     /translation="MVEAQATARGVRTSAQKAGLVCELIRGKNVNQALATLQFTRKTI
                     ARDLAKVLRSAVANLQQKDGFSGDIDRLYVTACYADQGPSMKRVRPAPMGRAYRVVKR
                     TAHLTVRVAERPLKIARVGTDGDAAAAAARPARAKKTTARTAAAKA"
     gene            1310..1999
                     /gene="rpsC"
                     /locus_tag="VM841_09020"
     CDS             1310..1999
                     /gene="rpsC"
                     /locus_tag="VM841_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HVL90361.1"
                     /translation="MGQKVHPYGFRLGFNKTWRSRWFSDRDYAKLLHEDLALRQNLKK
                     RFSHAGVSRIETERAANNLKIDIYTSRPGIIIGRKGTEVDKLKQEIQKRTSRAVFINI
                     QEIQKPELEAQLIAESVAVQLEKRVAFRRAMRKAVESALRFGARGIKVRVSGRLNGAE
                     IARSEWYLHGQLPLQTLRADIDYGFAEANTTYGQIGVKVWLYKGERLVPRAGREEEYR
                     DRAPRRPERRA"
     gene            2096..>2410
                     /gene="rplP"
                     /locus_tag="VM841_09025"
     CDS             2096..>2410
                     /gene="rplP"
                     /locus_tag="VM841_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HVL90362.1"
                     /translation="MLMPKKVKYRKQQRGRMAGKAWRGSDVSFGDYGLKAMEPAWITD
                     RQIEAARVAMTRFIKRGGKIWVRVFPDKPITKKPQETRMGKGKGAPEQWVAVVRPGRV
                     LFE"
BASE COUNT          461 a          738 c          903 g          308 t
ORIGIN      
        1 ctacgccgac ggcgagaagc gctacatcct gcagcccgcg ggggtgaagg tgggcgacac
       61 ggtggtggcc ggcgacaatg tcgacatcct gccgggcaac tgcctgccgc tgcggagcat
      121 tccgctcggc acgacgatcc acaacgtgga gctgcgtccg ggcaaggggg ggcagctcgc
      181 gcgcagcgcc ggctcctcgg tgcagctggt cgcgcgcgag ggcgaatacg cgtcggtgcg
      241 cctcccctcg ggcgagatcc ggaagatcaa cgtcgagtgc gtcgcgacgg tcggtcaggt
      301 cggcaacctc gatcacgaga acgtgtcgat cggcaaggcg ggccgcagcc gctggctcgg
      361 caagcggccg cacgtgcgcg gcgtggcgat gaacccggtc gatcacccgc tcggcggcgg
      421 tgaaggcaag acgtcgggcg gccgtcaccc ggtgacgccg tggggccagc cgaccaaggg
      481 gttcaagacg cgccgccgga agggcacgga tcgcttcatc gtgcagcgca ggacgaagta
      541 aatgagcaga tcactgaaga agggcccgtt tgtcgacacg ccgctcctcg agaagctcga
      601 ggcgatgaac cgggcgaacg agaagaaggt catcaagacg tggtcgcgcc gctcgacggt
      661 cgtgccggag atggtgggcc acacgatggc ggtgcacaac gggaagaagt tcatcccggt
      721 gtacgtgacc gagaacatgg tgggccacaa gctcggcgag ttcgcgccga cgcggacgtt
      781 caaggggcac accaccaaga cggaaaaggc cgcgacggcc gcgccggccg gcggcaaggc
      841 gtaaggactg gcgatggttg aagcacaggc aacggcgcgc ggcgttcgca catcggcgca
      901 gaaggcggga ctggtgtgcg agctgatccg cggcaagaac gtgaaccagg cgctggcgac
      961 gctgcagttc acgcgcaaga cgattgcgcg cgatctggcg aaggtgctgc gctcggcggt
     1021 cgcgaacctg cagcagaagg acggcttcag cggcgacatc gatcgcctct atgtgaccgc
     1081 ctgttacgcg gaccaggggc cgtcgatgaa gcgggtgcgt ccggcgccga tgggccgcgc
     1141 gtaccgcgtc gtgaagcgca cggcgcacct gacggtgcgc gtcgccgagc ggccgctgaa
     1201 gatcgcgcgg gtggggacgg acggcgatgc ggcggcggca gcagccaggc cggcgcgcgc
     1261 gaagaagacg acggcccgca cggccgccgc gaaggcataa ggagagatcg tgggccagaa
     1321 ggttcatccg tacggattcc ggctggggtt caacaagacc tggcgctcgc gctggttctc
     1381 ggaccgtgac tacgcgaagc tgctgcacga ggatctcgcc ctgcgccaga acctcaagaa
     1441 gcgcttctcg cacgcaggcg tttcgcgcat cgagaccgag cgcgcggcga acaacctgaa
     1501 gatcgacatc tacacctcgc gcccggggat catcatcggc cgcaagggga ccgaggtcga
     1561 caagctgaag caggagatcc agaagcggac cagccgcgcc gtcttcatca acatccagga
     1621 gatccagaag ccggagctcg aggcgcagct gatcgccgag tcggtggccg tgcagctgga
     1681 gaagcgcgtg gcgttccgcc gcgcgatgcg caaggcggtg gagtcggcgc tgcgcttcgg
     1741 cgcgcgcggc atcaaggtcc gcgtctccgg gcggctgaac ggcgccgaaa tcgcgcgatc
     1801 cgagtggtac ctgcacgggc agctgccgct gcagacgctg cgcgccgaca tcgactacgg
     1861 cttcgcggaa gcgaacacga cctacgggca gatcggcgtg aaggtgtggc tctacaaggg
     1921 cgagcgcctg gtgccgaggg cgggccgtga agaggaatac cgggatcgcg cgccgcgcag
     1981 accggaacgg cgggcgtaag gcgacaaaca ggcgttcggc tcttggcgtt cggctctggt
     2041 cccgagccga gagccccgag cccagagcca tcgcggatag acgagggtta ccggcatgtt
     2101 gatgccaaaa aaagtgaagt accggaagca gcaacgcggc cggatggcgg gcaaggcgtg
     2161 gcgcgggtcg gatgtgtcgt tcggcgacta cgggctgaag gcgatggagc cggcctggat
     2221 caccgaccgg cagatcgagg cggcgcgtgt cgcgatgacg cgcttcatca agcgcggcgg
     2281 caagatctgg gtgcgcgtgt tcccggacaa gccgatcacc aagaagccgc aggaaacgcg
     2341 gatgggcaag ggcaagggcg cccccgagca gtgggtcgcc gtcgtccgtc ccggccgcgt
     2401 cctgttcgag
//
LOCUS       DATLFO010000224         8270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_86993, whole genome shotgun sequence.
ACCESSION   DATLFO010000224 DATLFO010000000
VERSION     DATLFO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86993"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VM841_09030"
     CDS             <1..569
                     /locus_tag="VM841_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90363.1"
                     /translation="SRPVEPGEDEDEDAPPSSPLRPATRIREVKVAGLPPLREPDPLP
                     EANWSAAVPDQPLEKVSGGSAMALRRAEVAIRMITGKPGETGAILDSGLDLLYSVRTD
                     PAVVERVEQAYEETRNRRKLGPLALALFAVDSRTAPEALLDAFATASKVVAERIRRAI
                     LACDPADIRPHRSLIQALPQSRRDQIGY"
     gene            698..1861
                     /locus_tag="VM841_09035"
     CDS             698..1861
                     /locus_tag="VM841_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HVL90364.1"
                     /translation="MAEVEIGIGKTGRRAYGFDDISIVPSRRTRDRDDVDISWEIDAY
                     RFGLPMLASAMDGVVSPQTAIAIGKLGGLAVLNLEGLWTRYEDPTSIYEKIASLPDHE
                     ATPYMQQVYQEPIKDELVYKRVAEIKGAGVTAAASLTPQRVERYYKLAMDAELDILVI
                     QGTVVSAEHVSTRTEPLNLKEFIASLDIPIIVGGCASFETALHLMRTGAAGVLVGVGP
                     GAACTTRGVLGIGVPQATAIADAAGARMRHLQETGRYVHVIADGGMRTGGDVAKAIAC
                     GADAVMIGSPLTRAKEAPGQGFHWGMATFHPELPRGTRVQTKLVGTMEEILVGPAPVN
                     DGTFNLFGALRTSMADCGYQNIREFQRVEVCVAPSFLTEGKSYQRAQGVGMGK"
     gene            complement(1923..2651)
                     /locus_tag="VM841_09040"
     CDS             complement(1923..2651)
                     /locus_tag="VM841_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90365.1"
                     /translation="MKKTVFVLAFGAALGLLAAGLPAAASHSWGNYHWARTSNPFTLK
                     LGDNVTSEWDTYLVEASNDWNPSKVLDTTIVAGQAGNTKTCKTTAGRVEVCNAKYGNN
                     GWLGVAGISVSGGHITAAYVKLNDTYFTKPTYNKPEWRRLVMCQEIAHAFGLDHQDEN
                     FNNANLGTCMDYTNTPLGPPSNERPNAHDFDQIEAIYSHTDSTTTVGASAPGGPGNKG
                     GSSVRHEQVDANGNGAVTWTFYVR"
     gene            2791..3624
                     /locus_tag="VM841_09045"
     CDS             2791..3624
                     /locus_tag="VM841_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90366.1"
                     /translation="MRRLPILVVLVLFLPACGAVESIRDDVIGTPGGTQGGAGGSGGS
                     GSGGSGGSGGSGGAGYDDAGGLGTMVRTYLRASPASRLVVEVDYVARRAPSSRALSHL
                     KGVLEEVADKPGGIRVEAGNEIPGGRDSWSLAHIKALEKTHRSARSGGDTATMWIVYL
                     DGRYADQEGALGVAYSASAAAIFRDRIGDATTALIDATAIERAVLTHEAGHILALINI
                     GYKSTIDREDPDHPHHSKNRKSVMYYAVEDVSISNILTGGPPSTFDADDKADLAMLKS
                     G"
     gene            complement(3621..4445)
                     /locus_tag="VM841_09050"
     CDS             complement(3621..4445)
                     /locus_tag="VM841_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90367.1"
                     /translation="MNAPRRFVPLTAVAVVAAYFALASISFDKGLPIRPVYDGVHGPT
                     TYAWVNPPPGQEEGNEKPQPASGRIGLDDRGVKNGTIATPDGQFVITIQEGSIPAKPG
                     QNSIKVDITPLDANKVGKPPADLSYDGNAYRVTAVYLPSNEPATIDAPSCPPDEQPKR
                     CATLVVRYAFGSTALYRRTGSTWTTVEGAQAIPSSLQIFGDSPALGTFVPAGPFTHGE
                     ASGGGSRTYIVVLASTVVAAAAAGFTRRGTIKRWWQRRGKKPSRPAPMKQPTKRRR"
     gene            complement(4494..4910)
                     /locus_tag="VM841_09055"
     CDS             complement(4494..4910)
                     /locus_tag="VM841_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90368.1"
                     /translation="MKITSMIASLSLLALAACGGGDAGAGAKSPTPNPRPSSTATISI
                     LQPAPGQVVEGTTLMVKVKIDGATVLEQASRDVRPDTGHVHLALDGETVTLLAGLEFE
                     LKDLKPGQHLLEASFAAADHGPFNPPVVTTTTFTVT"
     gene            complement(4916..6895)
                     /locus_tag="VM841_09060"
     CDS             complement(4916..6895)
                     /locus_tag="VM841_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HVL90369.1"
                     /translation="MIRRAGAFAALFAAFALGAVAAAPSAAAHAALRSSEPAAGAQLP
                     AAPGEITITFTEQTEPALSSIRVLDSTGSAYERGKPAPDGGDALTLRVAVESLNEGVY
                     TVLWRVVSRVDGHATAGSFAFGVGVSPAGAPPPPAAAEPETVERSSLEMLGRFLMFAG
                     IVVVIGGAWFALFALREHEPRRDRRLLAYTAAGWVVAVAGVPLLAEAQRRAAGAGFGD
                     LAGTTIGRALVWRGVGVLAAGAGIAVAILARGTARRAALVVAAVAAAAATFVHVQAGH
                     ASATANPGAQVASQLVHVVAVSLWVGGFGALLAAIRGASSPDKTRAIKRYSATAGVTL
                     FIVLATGVARSLGELESLGDLTSTGYGRAALIKIAIFAAITALGAVNRFRNVAKAPES
                     LRPLRSVVKVEAALGAAALVAAAILASLAPPSPSSAAGTGRSERLEISASDFATTTRA
                     RVTIEPGYAGVNEVTVALADYDTGEPIVGADVSLRFAYLDDTTVPPSSVPLTAAGGKG
                     RYAAQGANLSLDGRWQLTILVQRNGGSTEIPLTVATRCRVTPTPFEGQPTLYDAALAG
                     GRSVQMYAGPAASGKTEIHITLFDAQGTEEFVRSAGVTAAKDGDDPRRLPHRRFTDGH
                     FIATGELGRGSWRFDVTIATPDGTFKACFEETIGG"
     gene            complement(6892..7542)
                     /locus_tag="VM841_09065"
     CDS             complement(6892..7542)
                     /locus_tag="VM841_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90370.1"
                     /translation="MRHLSLRAALIAALMTVGYAAPALAHESRAVGGITMVVGWGTEP
                     AYAGFMNNVHLGLTRAGQPMNDVRAGEVKVEVSFGEQKITLDLEPAFAVGRFGTPGTY
                     RSPLLPTRAGKYTFRFSGSHDGQKIEGTFECGEQTFDCVSDPEAVMFPEKDPSVAELS
                     QRVEREFPRMTSQVASIRDDVGSTADSARLFGIIGIVIGVIALVVALMTSRGRKAA"
     gene            complement(7595..8188)
                     /locus_tag="VM841_09070"
     CDS             complement(7595..8188)
                     /locus_tag="VM841_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90371.1"
                     /translation="MNRSAAILRPAILGAGALAGLVGAHAINYWLVASDPIRREALLH
                     ATGHSWLPGVESFAVAAALASVAGALAFGWREAPSGRVRGFRATTGRLALAQAAGFAA
                     LEIGERFFAGVPLHHLSPLLLALGIGLQVLVACGIAATLGLLARAGARIAQALRPRLA
                     RPSQAPRALPRPVRGIAFRHLILTAADPVRGPPAAAP"
BASE COUNT         1237 a         2828 c         2941 g         1264 t
ORIGIN      
        1 gttcgagacc cgtcgagccc ggcgaggatg aggatgagga tgcaccgccc tcttccccgc
       61 ttcggccggc gacgcgcatc cgtgaggtca aggtcgcggg cctgccgccg cttcgcgagc
      121 ccgatccgct gcccgaggcc aactggtcgg ccgcggtgcc ggaccagccg ctcgagaagg
      181 tgtcgggcgg gtcggccatg gcgcttcgcc gcgccgaggt cgccatccgc atgatcaccg
      241 gcaagcccgg tgagaccggc gcgatcctgg actccgggct ggacctgctc tactcggtgc
      301 ggaccgatcc cgcagtggtc gagcgggtcg agcaggccta cgaggagacg cgaaaccgcc
      361 gcaagctcgg gccgctggct ctggcgctgt tcgcggtgga cagccgcacg gctcccgagg
      421 ccctccttga tgccttcgcg accgcctcga aggtcgtcgc cgagcgtatt cgccgcgcga
      481 tcctggcgtg cgacccggcc gacatccgtc cgcatcggtc actgatccag gccttgccgc
      541 agtcccgccg cgaccagatc ggttactagc ccgcgaatac gggccaaatc aggccggttc
      601 gtgtcgcacc cgccctcgcc gtgggttacg ctttccgatg acctgcagct ccgaaacggg
      661 ggcgcgggaa cgggcgatga gcgaggtgga ggggtccatg gcggaagtag agatcgggat
      721 cggcaagacc gggcgacgag cgtacgggtt cgacgacatc tccatcgtgc cgtcgcgacg
      781 cacgcgcgac cgcgacgacg tggacatctc gtgggagatc gacgcgtaca ggttcgggct
      841 gccgatgctc gcgtccgcga tggacggcgt cgtgtcgccg cagacggcca ttgcgatcgg
      901 caagctcggc ggtctggccg tgctgaatct cgaaggcctc tggacccgtt acgaggaccc
      961 cacctcgatc tacgaaaaga tcgcctcgct gcccgatcac gaagccacgc cctacatgca
     1021 gcaggtctac caggagccca tcaaggacga gctcgtctat aagcgtgtcg ccgagatcaa
     1081 gggcgcgggc gtcaccgccg ccgcctcgct cacgccgcag cgcgtcgagc gctactacaa
     1141 gctcgcgatg gacgccgagc tcgacatcct ggtgatccaa ggaacggtcg tgtcggccga
     1201 gcacgtctcg acgcggaccg agccgctcaa cctcaaagag ttcatcgcct cgctcgacat
     1261 cccgatcatc gtcggtggct gcgcctcgtt cgagacggcg ttgcacttga tgcgcaccgg
     1321 cgcggccggc gtgctcgtgg gcgtcggccc gggcgcggcg tgcaccacgc gcggggtgct
     1381 cgggatcggc gtgccgcagg cgacggcaat cgccgacgcc gcgggcgcgc gcatgcggca
     1441 tctgcaagag accggacgct atgtgcatgt catcgccgac ggcgggatgc gcacgggcgg
     1501 cgacgtcgcc aaggccatcg cctgcggcgc cgacgcggtc atgatcggct cgccgctcac
     1561 gcgcgccaag gaagcgcccg gccagggctt ccactggggc atggcgacct tccacccgga
     1621 gctgccgcgc ggcacgcggg tgcagacgaa gctggtcggt acgatggaag agatcctggt
     1681 cggcccggcg ccggtcaacg acggcacctt caacctcttc ggcgcgctgc gcacatcgat
     1741 ggcagactgc ggctaccaga acatccgcga gttccagcgc gtcgaggtct gcgttgcgcc
     1801 ttcgttcctg accgagggca agagctacca gcgcgcccag ggcgttggca tgggcaagta
     1861 gagagcctcg aaaacgttcg agggggcgga tatccgcccc ctcgtttcgt ttctcgtccg
     1921 gctcagcgta cgtagaacgt ccaggtgacg gctccgttcc cgttcgcgtc cacctgctcg
     1981 tggcgaacgc tgctgccgcc cttgttgccc ggaccgcccg gcgccgacgc gcccacggtc
     2041 gtggtcgagt ccgtgtgcga gtagatcgcc tcgatctgat cgaagtcgtg cgcgttcggg
     2101 cgctcgttgc tcggcgggcc gagcggggtg ttcgtgtagt ccatgcacgt gccgaggttg
     2161 gcgttgttga agttctcgtc ctgatggtcc aggccgaacg cgtgcgcgat ctcctggcac
     2221 atcaccaagc gccgccactc cggcttgttg taggtgggct tggtgaagta agtgtcgttg
     2281 agcttcacgt acgcggcggt gatatgaccg cccgacaccg agatcccggc gacgccgagc
     2341 catccgttgt tgccgtattt cgcgttgcag acctcgacgc ggccggccgt ggtcttgcac
     2401 gtcttggtgt tgcctgcctg gccggcgacg atcgtcgtgt cgagcacctt cgacgggttc
     2461 cagtcgttgc tggcttcgac cagataggtg tcccactcgc tggtgacgtt gtcgcccagc
     2521 ttgagggtga acggattgct cgttcgggcc cagtgatagt tgccccagga atgggacgca
     2581 gcggccggca ggccggcggc gagcagtccg agcgccgccc cgaaggcgag cacgaaaacg
     2641 gttttcttca aaggatcccc ctgcgggcgg ttgcggaatt tccgccgtgc agagcaattg
     2701 tggggtcccg ttcgatgcaa agcaagggcc cggccggacc atttcctgat gccgattcgg
     2761 atcgcttgct gttgggtagc atgcctgggc atgagacgac tgccgattct tgtcgtgctg
     2821 gtcctgttcc tgccggcctg cggggccgtc gaatcgattc gcgacgacgt gatcggaacc
     2881 cctggaggga cccagggagg agcgggcgga tcgggtggct ccgggtccgg agggtccggc
     2941 ggctcgggcg gctcgggcgg ggccggctac gacgacgcgg gcgggctcgg cacgatggtg
     3001 cgcacctacc tgcgtgcttc gccggcgagc aggctcgtgg tcgaggttga ctatgtcgca
     3061 cggcgtgctc cgtcttcgcg ggccctgtcg catctgaagg gcgtgctcga ggaggttgcc
     3121 gacaagcccg gagggatccg tgtcgaggcc ggcaacgaga ttccgggcgg gcgcgactcg
     3181 tggtcgctcg cgcacatcaa ggcgctcgag aagacgcatc gctcggcgcg atccgggggc
     3241 gacaccgcaa cgatgtggat cgtgtacctc gacggccgct acgccgatca ggagggcgcg
     3301 ctcggcgtcg cctacagcgc ttcggcggcg gcgatcttcc gcgaccggat cggggacgcg
     3361 accaccgcgc tcatcgacgc gaccgcgatc gaacgggccg tgctcacgca tgaggccggt
     3421 catatccttg cgctgatcaa tatcggctac aagagcacga tcgaccgcga ggatccggac
     3481 catccgcacc attcgaagaa ccgaaagtcg gtgatgtact acgcggtcga ggacgtgtcg
     3541 atctcgaaca tcctcaccgg cggaccgccg tcgaccttcg acgcggacga caaggcggat
     3601 ctggcgatgt tgaagagcgg ctagcgacgc cgcttcgtgg gctgcttcat cggggccggc
     3661 ctcgacggct tcttgccgcg tcgctgccac caacgcttga tcgtgccgcg acgggtgaac
     3721 cccgccgcgg ccgccgccac gacggtcgag gcgagcacga cgatgtaggt ccggctgccg
     3781 cctccgctcg cttcgccgtg agtgaacggc ccggcgggaa cgaaggtccc cagcgcgggt
     3841 gagtcgccga agatctgcag cgacgacgga atcgcctgtg cgccctcgac cgtcgtccag
     3901 gtggagccgg tgcgccggta cagcgcggtc gagccgaacg cgtagcggac caccagcgtc
     3961 gcgcaccgct tcggctgctc gtcgggcggg caggacggag cgtcgatggt ggcgggctcg
     4021 ttcgacggca gatacaccgc ggtgacccgg taggcgttgc cgtcgtagga caggtccgcg
     4081 ggaggcttgc ctaccttgtt cgcgtccaat ggcgtgatgt cgaccttgat gctgttctgg
     4141 cccggcttgg cggggatgct gccttcctgg atggtgatca cgaactgccc gtcgggcgtg
     4201 gcgatcgtgc cgttcttgac gcccctgtcg tcgaggccga tgcgtcccga tgccggctgc
     4261 ggcttctcgt tgccttcctc ttggccgggc ggcgggttga cccaggcgta cgtcgtcggg
     4321 ccgtgcacgc cgtcgtagac cggccggatc ggcaagccct tgtcgaacga gatcgacgcg
     4381 agcgcgaagt aggccgcgac gaccgccacg gccgtgagcg ggacgaaccg gcgcggcgcg
     4441 ttcatgcgcg ctcctcgaag gcgttctgcg tcgatcggct caacgggatc ctcctacgtg
     4501 acggtgaacg tggtggtcgt aacgacgggc ggattgaacg ggccgtgatc cgcggccgcg
     4561 aacgacgcct ccagcagatg ctgtccgggc ttgagatcct tcagttcgaa ctcgaggccg
     4621 gccagcagcg taaccgtttc gccgtcgagg gcgaggtgca cgtggccggt gtccgggcgc
     4681 acgtcccgcg acgcctgctc gagcacggtc gcgccgtcga tcttgacctt gaccatgagc
     4741 gtcgttcctt cgacgacctg tccgggggcg ggctgcagaa tcgagatcgt cgccgtggac
     4801 gacggccgcg ggttcggcgt gggcgacttg gctcccgccc cggcatcgcc tcccccgcat
     4861 gcggcgagcg caagcagcga cagggaagcg atcatcgacg tgatcttcat aacggtcatc
     4921 ctccgatggt ctcttcgaaa catgccttga acgtgccgtc gggcgtggcg atggtgacgt
     4981 cgaagcgcca gcttccccgc ccgagctctc cggtcgcgat gaagtgcccg tcggtgaagc
     5041 ggcggtgcgg cagcctgcgg gggtcgtcac cgtctttcgc ggcggtcaca ccggccgatc
     5101 gcacgaactc ctcggtgcct tgcgcatcga acaaggtgat gtgaatctcg gtcttgccgc
     5161 tggcagccgg gccggcgtac atctggaccg agcgaccgcc ggcaagcgcg gcgtcgtaga
     5221 gcgtcggctg cccttcgaac ggggtcggcg tcacccggca gcgggtcgcc accgtcagcg
     5281 gaatctccgt cgagcctccg ttccgctgga ctaggatcgt gagctgccag cggccgtcga
     5341 gcgatagatt cgctccctgc gccgcgtacc gtcccttgcc gccggcggcc gtcaacggaa
     5401 cgctcgaggg tggaacggtg gtgtcgtcga ggtaggcgaa gcgcagcgag acgtcggcgc
     5461 cgacgatggg ctcgccggtg tcgtagtctg cgagcgcgac ggtcacttcg ttcacgcccg
     5521 cgtagccggg ctcgatcgtc acccgcgcgc gcgtcgtggt cgcgaagtcg gaggcgctga
     5581 tctcgagccg ctcgctgcgg cctgtgccgg cggcgctgct cggggagggc ggcgcgaggc
     5641 tcgccaggat cgcggcggcg acgagcgcgg ccgcgccgag ggccgcctcg accttcacca
     5701 ccgaccggag cggccgcagc gactcggggg ccttggcgac gttgcggaag cggttcacgg
     5761 ctccgagcgc ggtgatcgcc gcgaagatcg cgatcttgat cagcgcggcg cggccgtagc
     5821 cggtgctcgt cagatccccg agcgactcca gctcgcccag cgagcgcgcc acgccggttg
     5881 cgagcacgat gaaaagcgtc acaccggccg tggccgagta ccgcttgatc gcacgcgtct
     5941 tgtccggcga ggacgcgccg cggatcgcgg ccagcaacgc gccgaatccg cccacccaca
     6001 gcgataccgc cacgacatgc acgagctggc tcgcgacctg cgcgccgggg ttggccgtgg
     6061 ccgaggcatg gccggcctgt acgtgcacga acgtggcggc cgcggcggcc acggccgcca
     6121 cgaccagcgc ggcccggcgc gccgttccgc gcgccaggat cgcaaccgcg atccccgcgc
     6181 cggcggcgag cacccccacg cctcgccaga ccaaagcccg tccgatcgtc gtccccgcga
     6241 gatcgccgaa gccggcgccg gccgcgcggc gctgcgcctc ggccagcaac ggcacgcccg
     6301 cgacggccac cacccagcct gcggccgtgt aggcgagcag ccgccggtcg cgccgcggct
     6361 cgtgctcgcg cagcgcgaac agcgcgaacc acgcgccgcc gatcaccacc acgatgccgg
     6421 cgaacatcag gaaacgtccg agcatctcaa ggctcgaccg ttccacggtc tcgggctccg
     6481 cggcggcggg cggcggcgga gcacccgcgg gtgacacgcc cacgccgaac gcgaacgatc
     6541 ccgccgtcgc gtgtccgtcc acgcgcgaca ccacgcgcca gagcacggtg tagacgccct
     6601 cgttgagcga ctcgacggca acccgtagcg tcagggcgtc gccgccgtcc ggcgcgggct
     6661 tgccgcgttc gtaggccgag ccggtcgagt ccaggacccg gatcgacgac aacgcgggct
     6721 cggtctgctc ggtgaaggtg atcgtgatct cgcccggggc ggccggcagc tgcgcgcccg
     6781 ccgcgggctc ggacgagcgc agcgcggcgt gcgccgccgc gctcggagcg gcggcaaccg
     6841 ctccgagcgc gaacgccgcg aaaagcgccg cgaaggcgcc cgcgcgccgg atcatgcggc
     6901 ctttcgtccc cggctcgtca tgagcgccac gaccaaggcg atcacgccga tcacgatccc
     6961 gatgatgccg aacaggcgcg ccgaatccgc ggtcgagccg acgtcgtcac ggatcgaggc
     7021 gacctggctc gtcatccgcg ggaactcgcg ctcgacgcgc tggctcagct ccgcgaccga
     7081 gggatccttc tcggggaaca taacggcctc gggatcgctg acgcagtcga acgtctgctc
     7141 gccgcattcg aacgtgccct cgatcttctg gccgtcgtgc gagcccgaga atctgaacgt
     7201 gtacttgccg gcccgcgtcg gcagcagcgg ggagcggtag gtccccgggg tgccgaaccg
     7261 cccgaccgcg aacgccggct ccaggtcgag cgtgatcttc tgctcgccga aagagacctc
     7321 gaccttgacc tcgccggctc gcacgtcgtt catgggctgc ccggcgcgcg tcaggcccag
     7381 gtggacgttg ttcatgaagc ccgcataggc gggctcggtt ccccagccga cgaccatcgt
     7441 gatgccgccc acggcgcggc tctcgtgcgc gagcgcgggc gcggcgtatc caacggtcat
     7501 cagggccgcg atcaacgcgg cgcgaagcga tagatgcctc atctcggaat ctctcctgtt
     7561 ctcgggcgcc cctgggtcgg ggcgcgtcgt ccgcctacgg tgcggcggca ggagggccgc
     7621 ggaccgggtc cgcagcggtg agaatcagat gacggaacgc gatgccgcgt acgggccgtg
     7681 gcagcgcgcg gggcgcctgc gacggtcgag cgagccgcgg ccgcagcgcc tgcgcgatgc
     7741 gagcgccggc acgagccagc aatcccagcg ttgcggcgat gccgcacgca accaggacct
     7801 gcaagccgat cccgagcgcg agcagaagcg gtgagaggtg atgcagcggc acccccgcga
     7861 agaatcgctc cccgatctcg agcgccgcga agccggccgc ttgtgcgagc gcgagccttc
     7921 cggtggtggc ccggaagccg cggacccggc cggacggagc ctctcgccag ccgaacgcca
     7981 gggcaccggc gacggaggcc agcgcggccg cgacggcgaa ggactcgacg ccgggaagcc
     8041 agctgtggcc ggtcgcgtgc aggagagcct cgcggcgaat cgggtcggat gcgacgagcc
     8101 agtagttgat cgcgtgagcc ccgacgaggc ccgccagcgc tccggccccg aggatcgccg
     8161 ggcgcaggat tgccgcagac cgattcatgg cggcaagtct agcgtggcgg ccgattgtcg
     8221 tccgggggtg gccgatcgtc gtccgggggt ggccgatcgt cgtccggggg
//
LOCUS       DATLFO010000225         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87101, whole genome shotgun sequence.
ACCESSION   DATLFO010000225 DATLFO010000000
VERSION     DATLFO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87101"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VM841_09075"
     CDS             <1..1364
                     /locus_tag="VM841_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90372.1"
                     /translation="PARPAVVFTPEYRNAGDRVDEAFLDRDGTRIAANIRGKLWLGDV
                     SGRGWGVVGEYTALGWADQDRIAVLPSELYADGDPFAIEVGGGRGQRGEISLWRHPER
                     VRGNRSPGGAKIVYEFSDYSKPPSVPREGGAFESRKPSLTWPTETWIYDGTTATKTMV
                     AHRVDLTGGLERTAIPARPSPTSVWSPTGDLCLSVTYSPAETHSMVRILAGEGTTTSS
                     FRAVSPVWRAGWLDRERLWLAGPNGVSVRTLRGDAETAVTLPGMKRDAVISHDWTMSV
                     FTPANDIPVEDPDLVRAVGPAGKLRDVQTHYSDPKLGVAFTLPPSWSVREIRDGDPIG
                     PYPGVRLATNRFASDCCDGVFFTTTSDDVETVEEKLDRWAGFGEPDGPASVGGSPETV
                     GGRAFTYRRYDRSFESTLYVFLIGRVGEVTFIFRAPSSDLSGLTRAPDAYLVTASIQF
                     EAT"
     gene            1435..1800
                     /locus_tag="VM841_09080"
     CDS             1435..1800
                     /locus_tag="VM841_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90373.1"
                     /translation="MRHVDGAFELEPARCTRFGRGHTVHWIQANGSVRDPGVVEHGVV
                     TRVDFGMIEVRIGDELRRFRNHDTTTFHRLVRQLGRSVVIQDRWGLLKVPGPDGDRCF
                     SISAESEPWTPCTPRRTRS"
     gene            complement(1925..2698)
                     /locus_tag="VM841_09085"
     CDS             complement(1925..2698)
                     /locus_tag="VM841_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HVL90374.1"
                     /translation="MRAYGDIPELVLLGGLVPDLLCSSAPRSHQGTTDVDVQVDLEIH
                     GGSANAKRLEQALEAAGFKPDTERAWRWKDEVAAAMVVKIEFLADLDGVPNHHTFTFD
                     DCEMLGAQNLRGTGFAAKEWELRTITSNLDGESASVEFRVATLPAYLLAKTHAAHGRS
                     LSKDWYDIAYVLLHNDDGGPVAAARRVRDRFGDAIVGATATALHELLANFTDATSQGS
                     VAYATTMVGLHPDLDEAVLANDSVAALAAFMAELDLNRS"
     gene            complement(2742..3794)
                     /locus_tag="VM841_09090"
     CDS             complement(2742..3794)
                     /locus_tag="VM841_09090"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HVL90375.1"
                     /translation="MSEKGRLSDRVEAVTKAVLPMGLRVKVTKKGNGSIPLFDVAVGR
                     GAAVHRFVGCWVGEGWPGDVERLSGLTRHPDVVYAQQFSEGARAWLADHHLGWIDEAG
                     GAHVSLPSGLVVVRDQTRATVPAAVPQGWTSAMLTAAEAVLAGNPPVVMAIEQATALS
                     RGAATNALARLERVGLLSRPGALRGPASARQVVDIDAFIDAYAAAAAGFRAKQQRVLI
                     HRIWRDPLQALAREIAPSMAAARVKWAISGAAASTLLAPYLSDVTVIDLYVERSLIVA
                     QDHLAQLLHGRVVQKGHRIEVRELPTSMSANGPRIDGIQVALPGRVYADLLASGGRLA
                     EAAHHLREVHGAGPRS"
     gene            4003..>4255
                     /locus_tag="VM841_09095"
     CDS             4003..>4255
                     /locus_tag="VM841_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90376.1"
                     /translation="MMPKKRKDDGLDVLVHLFAQLPWWVPFSFAVAALVGVPPLGRAL
                     VNQPAYSLLWGLLGTLVAVALVGSGIFAQFEKVRRQRLDA"
BASE COUNT          763 a         1398 c         1369 g          725 t
ORIGIN      
        1 gcccggcgcg gccggccgtc gtcttcacgc ccgagtaccg caatgccggc gaccgcgttg
       61 acgaggcgtt cctcgaccgt gacggcacac gcatcgcggc gaatatccga gggaagctgt
      121 ggctcggcga cgtgtcggga cgcggctggg gtgtcgtcgg cgaatacacc gcgctcggat
      181 gggccgacca ggatcgcatc gcggttctgc cttcggagct ctatgccgac ggcgatccgt
      241 tcgcgatcga ggtcggcggc ggtcgcggcc agaggggcga gatctcgctg tggcgtcatc
      301 ccgaacgcgt tcgcggcaac cgctcgccgg gcggagcgaa gatcgtctac gagttcagcg
      361 actattcgaa gccaccaagc gtgcctcgcg aaggtggggc tttcgaatcg cgaaagccgt
      421 cgctgacgtg gccgaccgag acgtggatct acgacggcac aaccgcgacg aagacgatgg
      481 tcgctcatcg agtcgatctg acgggcggtt tggaaaggac ggcgattccg gcgagaccca
      541 gcccaacatc ggtgtggagt cccaccggcg acctttgtct atcggtgacg tactcgccgg
      601 ccgagacgca ctcgatggtt cgcatcctag ccggcgaggg cacgaccaca tcgtcgtttc
      661 gcgccgtgtc gccggtctgg cgagcaggct ggctcgaccg cgaacgcctg tggcttgccg
      721 ggccgaatgg cgtgtcggtt cgaacgctgc gtggcgacgc ggagaccgcc gtgacgcttc
      781 ccggcatgaa acgcgacgcc gtcatcagcc atgactggac catgagcgtc ttcacacccg
      841 ccaacgacat tcccgtcgag gatcccgatc tggttcgcgc cgtcggcccc gcggggaagc
      901 tacgcgacgt gcagacgcac tacagcgatc cgaagctcgg tgttgccttc acgcttccgc
      961 ccagctggag cgtgcgcgag atccgcgacg gagatccgat cggcccgtac ccgggcgtgc
     1021 gtctggcaac gaatcggttc gcgagcgact gctgcgacgg cgtgttcttt accacgacca
     1081 gcgacgatgt cgagacagtc gaggagaagc tggatcgttg ggccggcttc ggcgagccgg
     1141 acggaccggc cagcgtcggc ggcagcccgg aaacggtcgg cggccgcgca ttcacctatc
     1201 gccgctacga cagatcattc gaatcaacgc tctacgtgtt cctcatcggc cgggtaggag
     1261 aggtgacatt catcttccgc gccccatcgt ccgacctcag tggtctcacc cgagccccgg
     1321 acgcatacct cgtcaccgcc agcattcagt tcgaggcaac atgacgggcg acgggccatg
     1381 gcgttgtcac acccacctcg tacgatcagc tacgcgggag cgaggtgatc ggtgatgcga
     1441 catgtggatg gcgcgttcga gcttgagccg gcgcgctgca cgcggttcgg gcgcggccac
     1501 accgtccatt ggatccaagc caacggaagc gtccgagatc ccggagtcgt cgagcacggc
     1561 gtcgtcaccc gcgtggactt cggcatgatc gaggttcgga tcggcgacga gctgcgccgc
     1621 ttccgcaatc acgatacgac gaccttccat cggctcgtgc gacagctcgg ccgctcggtc
     1681 gtcatccaag accgatgggg gctcctcaag gtccccggcc ccgacggcga ccggtgcttc
     1741 agcatctcgg ccgagagcga accgtggaca ccctgcacgc cccgacgaac ccgcagttga
     1801 gcgccgcgtc gtcgcgacac aaagagaaac acgagactgc agggggatcc cgcgccgcct
     1861 ccacgccgcc tctcggctcc ccatttcctt ctttctacgt cttgggtccc gcgcgtgccc
     1921 atcatcaaga tcgattcaga tccaactcgg ccatgaaggc ggcaagggcc gcgaccgagt
     1981 cgttggcgag cacggcttcg tcgaggtcgg gatgcaaacc caccatcgtg gtcgcgtaag
     2041 caacactccc ctgagacgtc gcatccgtga agttggccag cagctcgtgg agcgccgttg
     2101 ccgtcgcgcc gacgatcgcg tctccgaacc gatcgcgaac gcgacgagcc gcggcgacgg
     2161 gtcctccgtc gtcgttgtga agcaggacgt acgcgatgtc gtaccaatcc ttcgaaagcg
     2221 agcgcccgtg agcagcgtgc gtcttcgcca acagataggc gggcagcgtc gcgacgcgaa
     2281 actcgacgct tgcggactca ccatccagat tcgacgtgat ggtccgaagc tcccactctt
     2341 tggccgcaaa acccgtaccg cgcagatttt gagcaccgag catctcgcag tcatcaaacg
     2401 tgaaggtgtg atggttgggg acgccgtcaa gatcggcgag aaactcgatc ttgacgacca
     2461 tcgccgccgc gacctcgtct ttccaacgcc atgctcgttc cgtatccggc ttgaatccgg
     2521 ccgcctccag agcttgttcc aaacgcttcg cgttggcgga gcctccgtga atctccagat
     2581 cgacctggac atcgacatcg gtcgtgccct ggtgagatcg cggtgcactg ctgcagagaa
     2641 ggtcgggcac gagtccaccc aagaggacca gttcagggat gtctccgtag gcgcgcacga
     2701 gccggacgag cgcgcgttcc gcgagtgctc tcgtttcgcg actacgaacg gggtccggca
     2761 ccgtgaacct ctcggagatg atgcgccgct tcggcgaggc gcccgccgct tgccagcagg
     2821 tcggcataca cgcgtcccgg cagcgcaact tgtatcccat cgatgcgggg accgttggcg
     2881 ctcatcgacg tcggcagctc gcgcacctcg attcggtggc ccttctgaac cacgcggcca
     2941 tggagaagtt gggcgaggtg gtcttgggcg acgatgaggg atcgttcaac gtagagatcg
     3001 atgaccgtga cgtcactcag gtacggcgca agcagcgttg acgcggcggc accgctgatc
     3061 gcccacttca cccgtgccgc cgccatcgaa ggagcgatct cgcgcgcaag cgcttgcaga
     3121 gggtcacgcc aaatccgatg gatcaacacg cgctgctgct tggctcgaaa gccggcggca
     3181 gcggcagcgt acgcgtcgat gaacgcgtcg atgtcaacga cctgccgcgc cgaggcgggg
     3241 ccgcgaaggg cccccggccg cgacagaagg ccgacgcgtt caagacgcgc gagtgcattg
     3301 gtcgcggcgc cgcgcgatag agcggtcgcc tgctcgatcg ccatgacgac cggggggttt
     3361 ccggcaagga ccgcctcggc cgcggtgagc atcgccgaag tccacccttg cggtacagcc
     3421 gcgggtacgg tggcacgagt ctgatcacgg acgacgacga gaccggaagg caagctgaca
     3481 tgtgcgcctc ccgcttcatc gatccagccc agatggtgat cggccagcca ggccctggct
     3541 ccctcggaga actgctgggc atagacgacg tcgggatggc gggtgaggcc ggacagacgc
     3601 tcgacgtccc cgggccaccc ttctccgacc cagcagccga cgaatcgatg gacggcggct
     3661 ccgcggccga cggccacgtc aaagagggga atagagccgt ttcccttttt ggtcaccttg
     3721 acccgaagac ccatgggaag cacggctttc gtgacagcct cgactcgatc cgacagcctg
     3781 cccttctcgc tcatacttca gagagtagca tacttcactc gccggtgaag tacatgtatt
     3841 cggtggagta tcggcgcctc aaagcggctc ccaacataag gccggcccca ccgggccagt
     3901 ctttcctgca cccccttgtg ctgtcttccc gcacgactgt caacgcgacc agttgtccta
     3961 aagccgcggt ggcgacattc cgatcaggaa ttgggggagt tgatgatgcc gaagaagaga
     4021 aaagacgacg gcctggacgt gctggtccat ctgttcgcgc aactgccgtg gtgggttccg
     4081 ttcagtttcg ccgtcgcggc attggtggga gttccgccgc tcggcagagc tctggtcaat
     4141 cagcccgcct acagcctcct gtggggactt ctcgggacgc tagttgccgt cgcactggtc
     4201 ggctcgggaa tcttcgccca gttcgagaaa gtccgccgtc aacggctcga cgcgc
//
LOCUS       DATLFO010000226         6993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_142889, whole genome shotgun sequence.
ACCESSION   DATLFO010000226 DATLFO010000000
VERSION     DATLFO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142889"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VM841_09100"
     CDS             complement(<1..977)
                     /locus_tag="VM841_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL90377.1"
                     /translation="MSIVPRRGLTGFALTLALLAGLLAFAPAQAVPFCNIDLTTSPNC
                     YVAVDDGTVIAMTVRVPADCRSGARECPTIFEMAGYENGTAEGLTTTGEFFGTGAPLA
                     RDSRQLTEIFDGDYATVHASVRGTGCSQGEFDLFSIRSAQDGAWLIDNWIAKQDWSDG
                     KVGLMGHSYSGITGTMIAQRQPKSLVAMTVSGLIDDLYRGITYLGGVFNALFPPLWTL
                     GVRPAYDVVGGLMQGVGRPIWREIPALNPYNKTFDRAQAVQCAGSVVAKNRTTINDPV
                     IQGLLSPTDGTWWQIRSLITFIDRIDVPIHIAGSYQDEQTGARGFTHLW"
     gene            complement(1091..1984)
                     /locus_tag="VM841_09105"
     CDS             complement(1091..1984)
                     /locus_tag="VM841_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HVL90378.1"
                     /translation="MRILRTPDDRFEDLPGFPFLPNYLETGGLRIHYVDEGPKDGLPV
                     LMLHGEPSWSYLYRKMIPVFTASGFRAVAPDLVGFGRSDKPAGRTDYTYERHVAWMLA
                     VIEQLDLRGITLVCQDWGGLIGLRLAAEHPDRFARIVATNTFLPTGDMPAGEAFLRWR
                     EYSQTAPDFDIGAIVAKGCATELRPEITAAYDAPFPSDEYKAGARQFPVLVPVTPDDP
                     AAAANRAAWEVLSRWEKPFLTAFGDQEKITRGADRFFQSAIPGAAGRPHTTIEGAMHF
                     IQEDKGEELARVTVEFIDATC"
     gene            2066..2653
                     /locus_tag="VM841_09110"
     CDS             2066..2653
                     /locus_tag="VM841_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HVL90379.1"
                     /translation="MNRFFVNQKAKLIELTNEYAILDEDGSVVGAIRQEGQSKARKIL
                     RAFTSVDQFLTHHLSVYDAAGTKILALTRPAKLVKSRVLVADGAGAPVGSIEQQNVIG
                     KIRFAFVGAQGEVLGAVNAENWRAWNFSIADAAGTEVGRITKKWSGIGKEIFTTADNY
                     MVELDASLSGPLRLLALAAAAGVDTALKQDARGLG"
     gene            complement(2650..4035)
                     /locus_tag="VM841_09115"
     CDS             complement(2650..4035)
                     /locus_tag="VM841_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017975585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL90380.1"
                     /translation="MKVEQFVLESLGHASYFLSSERTGEAIVVDPRRDVEPYLEAERR
                     YGARIVAVLDTHQHNDYASGIVELARRTGARMLASAEANVKYDSRAVADGEELTFGEM
                     RLRVIHTPGHTPEHVSLLAFDGEDEPAMLISGGALLVGDLARPDLLGGPEEKRESART
                     FCRTLQEKILTLPDHVLVYPTHVAGSLCGGNIGSRYSTTVGYERRTNPVLAAVAARDV
                     FVEECIRMDDLPAVPPYWRRMRGLNQEGPEPVGAFPPPPALQVGDFAERIEGGAVVLD
                     ARSPEAFAGTHIPGSLNVGLSTSFPTWAGTIVPEDRDILLVLDDPSDLAEVVWSLIRI
                     GYRVPSGWLAGGISSWRTSAREIGFVPHIEVRDLAGRLNDFTVLDVRQPGEWAGGHVE
                     GALFVTGAEVPERLDEIPEGKPVAVMCGSGYRSSVISSLLKHHGRADVMNILGGMSAW
                     HSARLPVTKES"
     gene            complement(4046..4405)
                     /locus_tag="VM841_09120"
     CDS             complement(4046..4405)
                     /locus_tag="VM841_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVL90381.1"
                     /translation="MGLFRSRASNDLDAQELSKISENGGEVIDVRTYHEWEAGHLPFA
                     RHIPIDELLARAGELDKERLNVFICHVGARSQAASDALGRNGFKTATVPGGMEAAKAV
                     GLPVIRSDGSPGRVISA"
     gene            4560..6473
                     /locus_tag="VM841_09125"
     CDS             4560..6473
                     /locus_tag="VM841_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HVL90382.1"
                     /translation="MIDHRDDPARSTHAVRGLSIALALIVAGIGRLLHAASDPAASVF
                     SHLGHVMADAIPALPLGLTAVLGGRWLSRRTGVSRPAAIAFVLAAGFVPAGAVHEALA
                     GASVAVHGHGAAAHRWSLAHGVRDAVPAFFAAFAAATLASFGSAFAAAAGRLRVASAR
                     VPRARIALALAALAATLPGRMPVAAASALPFSNPLVIPQERTGDDIAIEMREAEVQIL
                     PGAPTKMWTYDGTFPGPTIRRPSGVTTRVTFTNSLPEEAGSMTVHHHGNHSASTEDGQ
                     PASHLIPVGGSRTYTYDFVENDAPERAAMQWYHDHRMDVTGRNVWNGLAGMVILDDEV
                     DADLPLPEGAFDVPLMFVDRSFDANNQLSYRFNANGVRGTKMLVNGVVQPRFDVAGAK
                     YRLRLLNASNISEFTFAFADGTEMLQIATEGGLLPAPVPRTRIKLAPAERAEVVVDFT
                     GMNGRSLVLQNVEGSPLLRQVMRFDVGPNVPDASRVPEVLRPLEPAPEVSIENTRVWA
                     LTQSLAGDLVWTINGLEYDAERIDTAPLNPVLGSAERWIFVNTTPVNHVMHIHDVDWW
                     LIRRTSPDPYPADALLAEQGLKESFLVRPNEVVEIVSRFTDHLGTYVFHCHVLEHEDR
                     AMMAQFEVVLSRP"
     gene            6623..>6993
                     /locus_tag="VM841_09130"
     CDS             6623..>6993
                     /locus_tag="VM841_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90383.1"
                     /translation="MSKVRRRLVFASFAAVLLLAAALPATGRAQAHTVRVIVQASSAE
                     AAADAVRSAGGRVERPLSIVDGVAAVASSAAVEWIRRDPQVRAVTDNGPVRFFQESSK
                     NLSTYKHTFPLSTAASSLWSEG"
BASE COUNT         1071 a         2391 c         2399 g         1132 t
ORIGIN      
        1 tcccacaggt gcgtgaaccc gcgggcgccc gtctgctcgt cttggtacga gccggcgatg
       61 tggatcggca cgtcgatgcg atcgataaag gtgatcagcg agcggatctg ccaccaggtg
      121 ccgtcggtcg gggagagcag gccttggatg accggatcgt tgatcgtggt gcggttcttc
      181 gccacgacac tgccggcgca ctgaaccgcc tgcgcgcgat cgaaggtctt gttgtaggga
      241 ttcagcgccg ggatctcgcg ccagatcggc cggcccacac cctgcatcaa cccgccgacc
      301 acgtcgtagg cgggacgcac gccgagcgtc catagcggcg ggaacagtgc gttgaacacg
      361 ccacccaggt aggtgatgcc gcgatacagg tcgtcgatca gccccgacac ggtcatcgcg
      421 acgaggctct tgggctggcg ctgcgcgatc atcgtcccgg tgatgccgct gtaggagtgg
      481 cccatcaggc cgaccttgcc gtcggaccag tcctgcttcg cgatccagtt gtcgatcagc
      541 catgcgccgt cctgcgcgct gcggatgctg aacaggtcga actcgccctg cgagcagccg
      601 gtgccgcgca cgctcgcgtg cacggtcgcg tagtcgccgt cgaagatctc ggtgagctgc
      661 cgtgagtcgc gggcgagcgg cgcgccggtt ccgaagaact cgccggtcgt ggtcagccct
      721 tcggcggtgc cgttttcgta gccggccatc tcgaagatgg tcgggcactc gcgcgctccg
      781 ctgcggcagt cggccggaac ccggaccgtc atcgcgatga cggtgccgtc gtccaccgcg
      841 acgtagcagt tgggcgaggt ggtcagatcg atgttgcaga acgggacggc ctgcgccggc
      901 gcgaaggcca gcaggccggc cagcaaggcc aatgtcagcg cgaacccggt caggccgcgg
      961 cgcggaacga tcgacatgat gcggagctcc tccctcatcc ggccggccct cggcgagccc
     1021 ggcgggtctc ggggaagact tcgcgcaatc aagcccgaat cctgccgtga tgcgccgatt
     1081 ccgaatccgg tcagcaggtg gcgtcgatga actcgaccgt gacgcgggcg agctcctcgc
     1141 ccttgtcctc ctgaatgaag tgcattgcgc cttcgatcgt cgtgtggggg cggccggccg
     1201 caccgggaat cgccgactga aagaagcggt cggcgccgcg ggtgatcttc tcctggtcgc
     1261 cgaaggcggt caggaacggc ttttcccacc tggacaggac ctcccacgcg gctcggttgg
     1321 cggccgccgc gggatcgtcg ggcgttaccg ggaccagcac gggaaactgg cgagccccgg
     1381 ccttgtactc gtcggagggg aacggcgcgt cgtaggcggc ggtgatctcc gggcgaagct
     1441 cggtcgcgca tcccttggcg acgatcgcgc cgatgtcgaa gtccggcgcg gtctgcgagt
     1501 actcgcgcca gcgcagaaac gcctcgcccg ccggcatatc cccggtcggc aggaacgtgt
     1561 tcgtcgcgac gatgcgggcg aagcggtcgg gatgctcggc ggcgagccgc agcccgatca
     1621 ggcctcccca gtcctggcac accaaggtga tgccgcgcag gtcgagctgc tcgatcacgg
     1681 cgagcatcca ggcgacgtgc cgctcgtagg tgtagtcggt ccggccggcc ggcttgtcgc
     1741 tgcggccgaa acccaccaag tcgggagcca ccgcgcggaa gccggacgcg gtgaacacgg
     1801 gaatcatctt gcggtacagg taggaccacg acggctcgcc gtgcagcatc agaaccggca
     1861 gcccatcctt gggaccttcg tccacgtagt ggattcgcaa gccgccggtc tcgagatagt
     1921 tcggaaggaa cggaaagccc ggcaggtcct cgaagcggtc gtcgggcgtg cgcagaatcc
     1981 gcatggcgtc gtcctccgtc gcgaagtatg gtgcctggcc ggaactctat tcgagggagg
     2041 ctccacggtg ccgggcttgc tcaccatgaa tcggttcttc gtcaatcaga aggccaagct
     2101 catcgagctc accaatgagt acgccattct cgacgaggac ggaagcgtgg tgggtgcgat
     2161 ccgccaggag ggacagtcga aggcgcgaaa gatcctgcgc gccttcacct cggtcgatca
     2221 gttcttgacg catcacctgt cggtctacga tgcggcgggg acgaagatct tggcgctgac
     2281 gcgacctgcc aagctggtga agtcgcgggt gctcgtggcc gacggcgccg gcgcgccggt
     2341 cggctcgatc gagcagcaga acgtgatcgg aaagattcgc ttcgcgttcg tcggcgcgca
     2401 gggcgaggtg ctcggcgcgg tcaacgcgga gaactggcgg gcgtggaact tctcgatcgc
     2461 cgacgccgcg ggcaccgagg tcggacgcat caccaagaag tggagcggca tcggcaagga
     2521 gatcttcacc accgccgaca actacatggt cgagctcgat gcgagcctgt cgggaccgct
     2581 ccgcctgctc gcgctcgcgg ccgctgcggg cgtggacacc gcgctcaagc aggacgcacg
     2641 cgggctcggc tagctctcct tcgtcaccgg cagcctcgcg ctgtgccacg cgctcatccc
     2701 gccgaggatg ttcatcacat cggcgcgtcc gtggtgcttg agcaggctcg agatcacgct
     2761 ggaccggtag ccgctcccgc acatgacggc gaccggcttg ccctcgggga tctcgtcgag
     2821 ccgctcgggc acctcggcgc cggtgacgaa cagcgcgccc tcgacgtggc cgccggccca
     2881 ttcgcccggc tggcggacat cgagcacggt gaagtcattg agacggccgg cgagatcgcg
     2941 cacctcgatg tgcggcacga agccgatttc acgcgccgag gtccgccacg acgagatgcc
     3001 gcccgcgagc cagccgctcg gcacgcggta gccgatacga atcaggctcc atacgacctc
     3061 ggccaggtcc gatggatcgt ccaggaccag caggatgtcg cggtcctcgg gaacgatcgt
     3121 gccggcccag gtcggaaacg acgtggacag tccgacgttg agcgagccgg ggatgtgcgt
     3181 gccggcgaac gcctccggcg agcgtgcgtc gagcacgacc gcgccgccct cgatgcgctc
     3241 ggcgaaatcg ccgacctgca gcgccggcgg tggcgggaac gcgccgaccg gctcgggacc
     3301 ctcctggttc aggccccgca tccgccgcca gtagggcgga acggccggaa ggtcgtccat
     3361 ccggatgcat tcctcgacga agacgtcgcg tgccgcgacg gccgcgagca ccgggttcgt
     3421 tcggcgctcg tagccgacgg tcgtcgagta gcggctcccg atgttgcccc cgcacagcga
     3481 gccggccacg tgcgtcggat acaccagcac gtgatccggc agcgtgagga tcttctcctg
     3541 cagcgtgcgg cagaaggtgc gggcggactc gcgcttctcc tcggggccgc ccagcaggtc
     3601 ggggcgagca aggtcgccga cgagcagcgc gccgcccgag atgagcatcg cgggctcgtc
     3661 ctcgccgtcg aatgcgagca ggctcacgtg ctcgggcgta tggccgggtg tgtggatcac
     3721 gcgcagccgc atctcgccga aggtgagctc ctcgccgtcg gcgacggcac ggctgtcgta
     3781 ctttacgttc gcttccgccg aggcgagcat ccgcgcgccc gtccgtcgcg cgagctcgac
     3841 gatgcccgac gcgtagtcgt tgtgctgatg cgtgtcgagc acggcgacga tgcgagcccc
     3901 gtaccgacgc tcggcctcga ggtacggctc gacgtcgcgg cgcggatcga ccacgatcgc
     3961 ctcgccggtc cgctcgctcg aaaggaagta cgaggcgtgt cccaagcttt ccagcacgaa
     4021 ctgctcgacc ttcacggcac gctccttatg cgctgatgac ccggcccggc gacccgtcgc
     4081 tgcggatgac cggcaaaccg acggctttgg cggcctccat gccgcccgga accgtcgccg
     4141 tcttgaaccc gttgcgtccg agcgcgtcgc tcgccgcctg gcttcgcgcg cccacgtggc
     4201 agatgaagac gttcaggcgc tccttgtcga gctcgcccgc gcgcgcgagc aactcgtcga
     4261 tcgggatgtg ccgcgcgaac ggcagatgac cggcctccca ttcgtggtag gtgcgaacgt
     4321 cgatgacctc gccgccgttt tcggagatct tgctcagctc ctgcgcatcg aggtcgttgc
     4381 ttgcgcggct ccggaacaat cccatgggtc tcccttgctc gaaccgggtg gtcttcgttg
     4441 agcctaccca tcgccgtccc ctcgacgctc cctccgaagg agggaatcga agcgcgatat
     4501 gtccgattca gcccgcatgc gacaggggcc ggcgcccgcg gcgtcgaatt tgagcggcga
     4561 tgatcgatca ccgcgacgac cctgctcgct cgactcacgc cgtccgcggc ctgtcgatcg
     4621 ccctcgccct gatcgtcgcc ggcatcgggc ggctgttgca cgccgcgagc gatccggccg
     4681 cgtccgtgtt ctcgcacctc gggcacgtga tggcggacgc gatcccggcc ttgccgctcg
     4741 ggttgacggc cgtgttgggc gggcgatggc tgtcacggcg gaccggcgtg tcccgtcccg
     4801 ccgcgatcgc attcgtgctg gccgccggat tcgtgccggc gggcgcggtg cacgaggcgc
     4861 tcgccggcgc atcggtcgcg gtccacgggc acggcgccgc ggcacatcgc tggagtctcg
     4921 ctcacggggt tcgcgacgcg gttcccgctt tcttcgcggc cttcgcggcc gcaacgctgg
     4981 catcctttgg ctccgcgttc gccgcggccg cggggcggct gcgtgtcgcg tcggcccgcg
     5041 tgccacgcgc ccggatcgcg ctcgcgctcg ccgcgctggc cgcgacgctg ccggggcgga
     5101 tgccggtggc ggccgcgtcg gcgttgccgt tctccaaccc gctggtcatc ccgcaggagc
     5161 gcacgggaga cgacatcgcg atcgagatgc gcgaggcaga ggtgcagatc ctgcccggag
     5221 ccccgaccaa gatgtggacc tacgacggaa cgttcccggg tcccacgatc cggcggccca
     5281 gcggcgtcac cacgcgggtc accttcacca acagcctgcc cgaggaggca ggatcgatga
     5341 cggtgcacca tcacggaaac cactccgcct cgaccgagga cggccagccc gcctcgcacc
     5401 tgattcccgt cggaggatca cggacctaca cgtacgactt cgtcgagaac gacgcgcccg
     5461 agcgcgcggc gatgcagtgg taccacgacc accggatgga cgtgaccggc cggaacgtgt
     5521 ggaacgggct cgccgggatg gtgatcctcg acgacgaggt cgacgcggac cttccgctgc
     5581 ccgagggagc gttcgacgtg ccgttgatgt tcgtggaccg ttccttcgac gccaacaacc
     5641 agctgtccta ccggttcaac gcgaacgggg tgcgcggaac caagatgctc gtcaacggag
     5701 tcgtgcagcc gcgcttcgac gtcgcggggg cgaagtaccg gctgcgtctg ctcaacgcgt
     5761 ccaacatctc cgagttcacc ttcgcgttcg ccgacggaac cgagatgctc cagatcgcca
     5821 cggaaggcgg cctgctgccc gcgccggtgc cgcgaacgcg gatcaagctc gcgccggccg
     5881 agcgggccga ggtcgtcgtg gacttcaccg gcatgaacgg ccggagcctg gtgttgcaaa
     5941 acgtcgaggg ctcgccgctg ctgcggcagg tgatgcgctt cgacgtcggt ccgaatgtac
     6001 ccgacgcctc acgcgttccg gaggtgcttc gtccgctcga accggcaccg gaggtctcga
     6061 tcgagaacac gcgggtgtgg gcgctcacgc agtcgctcgc gggtgatctg gtctggacga
     6121 tcaacggcct cgagtacgat gcggaacgca tcgacaccgc gccgctcaac ccggtcctcg
     6181 gcagcgcgga gcggtggatc ttcgtgaaca ccaccccggt caaccacgtc atgcacatcc
     6241 acgacgtgga ctggtggctg atccggcgca cctccccgga tccgtatccc gcggacgccc
     6301 tgctcgccga gcaaggcttg aaggagtcgt tcctggtccg gcccaacgag gtcgtcgaga
     6361 tcgtctcccg gtttacggat catctgggca cctacgtgtt ccactgccac gtgctggagc
     6421 atgaggaccg ggccatgatg gcccagttcg aggtcgtgct ctcccggccg taggaggacc
     6481 gcgacgggga gggccgcgac ccgtcccacc tggggtttag cccctggttg gccggggtag
     6541 gacggttgct gggtcccgtg ggggaattcg gtgtccctgg ggtttcactc gcgtccgatc
     6601 gaacagccga ggtcgccaag ccatgagcaa ggtccgtcgc cgtctcgtat tcgccagctt
     6661 cgccgccgtt ttgttgcttg ccgccgccct tcccgccaca ggccgggcac aggcgcacac
     6721 cgtgcgggtc atcgtccagg catcgtccgc ggaggccgcc gcggacgcgg ttcgctcggc
     6781 cggaggccgc gtcgagcgtc cgctctcgat cgtcgacggt gtcgcggccg tcgcttcgag
     6841 cgccgccgtc gaatggatcc gcagggaccc gcaggtgcgc gccgtgaccg acaacgggcc
     6901 ggtccgtttc ttccaggaat cttcgaagaa cttatccacc tacaagcaca cctttccgct
     6961 gtccactgcg gcgtcctccc tatggtccga ggg
//
LOCUS       DATLFO010000227         2333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87204, whole genome shotgun sequence.
ACCESSION   DATLFO010000227 DATLFO010000000
VERSION     DATLFO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87204"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>2333
                     /locus_tag="VM841_09135"
     CDS             53..>2333
                     /locus_tag="VM841_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90384.1"
                     /translation="MAIGIIGIFAANAASLALPEIQRAFKLNLTSLLELAKWVGLLGT
                     LLAVPMGYLGDRARRVTMYAIGGIVNSIGTLFIGLAPTKAVLYAARSTSTVGDLGPEA
                     VGFSLIADYVPQEYRGQSFAFRAAGRSLGAIIAPITVGLLGQYYSWRLPFVIAGIAGA
                     IVSVWFFRMPEPIRGYHERKAWGAGEDVARRQQEPPSWGEAFRAAWGVRTLRRYAFAT
                     PFEEVGTLGFALFFPFFLARTHGVDILQRGIFGAILAFFGLMGLTVGGPLVDRIIGRN
                     PGRVVIISGVANAFNALSILGIIFAPNKWVVLLITAVFASLNSILAPARVAVISQVIP
                     PRIRNLGLATLAYWSLPVFFIFPVIGSVASSHGYRAGLLVFVPFQLIAAVLVMTAGPY
                     VEFDIRSATAAAMAAEEWRRAREEGRAKLLVLRSVDVHYDSVQVLFGVDLDIQEGELI
                     ALLGTNGAGKSTLLRAVSGTQEASGGAIVFDGRDITHVPPHEIAKHGIAQTPGGRGIF
                     PGLTVAENLQLAGWLYGEDTKHKDEVEQIFGYFPILRERLGQTAGSLSGGEQQMLTLA
                     QAFLARPKLLMIDELSLGLSPAIVEQLLGIVRAIHERGTTIIIVEQSVNVALTIAERA
                     IFMEKGEVRFDGPTSDLLGRPDIMRAVFLKGGRSLSVGAGPTETVKIREEERANILEV
                     RGLVKRYGGVAAVDGVGFVLREHETLGLIGPNGAGKTTVLEMISGFNPVDAGQVIYDG
                     RDVTMMTPDERANYGLIRRF"
BASE COUNT          336 a          812 c          781 g          404 t
ORIGIN      
        1 gcgtccgctc cgcctccgca gctcctatgg acgcacaccc cttgtgctca tcatggcgat
       61 cggcatcatc ggcatcttcg ctgcgaacgc agcgagcctg gccctgccgg agatccagcg
      121 cgcgttcaag ctgaacctga cgtcgctgct cgagctggcg aagtgggtcg gcctcctcgg
      181 gacactgctc gcggtcccga tgggatacct cggtgaccgc gcgcgccgcg tcacgatgta
      241 cgcgatcggc ggcatcgtga actccatcgg cacgctgttc atcgggctcg cgccgacgaa
      301 ggccgtgctg tacgccgccc gctcgacctc aacggtcggg gacctcgggc ccgaggccgt
      361 cggcttcagc ctcatcgccg actacgttcc ccaggagtac cgcgggcagt ccttcgcctt
      421 ccgcgcggcc gggcgctcgc tcggcgccat catcgccccg atcaccgtcg gactgctcgg
      481 ccagtactac tcctggcgcc ttccgttcgt catcgccggc atcgccggcg cgatcgtgtc
      541 ggtgtggttc ttccggatgc cggagccgat ccgcggctac cacgagcgca aggcatgggg
      601 cgccggcgag gacgtcgcgc gtcgccagca ggagccgccg tcgtggggcg aggcgttccg
      661 cgccgcatgg ggcgtacgca cgctgcgccg ctacgcgttc gcgaccccgt tcgaggaggt
      721 cggcacgctc ggattcgcgc tgttcttccc cttcttcctt gcgcgcacgc acggcgtgga
      781 catcctccag cgcggcatct tcggcgcgat cctggcgttc ttcggcctga tggggctcac
      841 cgtcggcggc cccctcgtgg accgcatcat cgggcgcaac cccggccgcg tcgtcatcat
      901 ctccggcgtc gcgaacgcgt tcaacgcact ttcgatcctt gggatcatct tcgcaccgaa
      961 caagtgggtg gtcctgctga tcaccgcggt cttcgcatcg ctgaactcga tcctcgcgcc
     1021 ggcgcgcgtg gccgtgatct cgcaggtgat cccgcctcgt atccgcaacc tcgggctggc
     1081 gacgctcgcc tactggagcc tcccggtctt cttcatcttc ccggtcatcg ggtcggtcgc
     1141 ctcgtcccac ggctaccggg cgggcctgct cgtcttcgtc cccttccagc tgatcgcggc
     1201 ggtgctggtc atgaccgccg gtccgtacgt ggagttcgac atccgctcgg ccaccgcggc
     1261 cgcgatggcc gccgaggagt ggcggcgcgc gcgagaggag ggccgggcga aactgctggt
     1321 cttgcgcagc gtggacgtcc actatgactc ggttcaggtc ctgttcgggg tcgacctgga
     1381 catccaagag ggcgagctga tcgcgctcct tgggaccaac ggagcaggga aatcgaccct
     1441 cctgcgagcc gtctccggca cgcaggaagc atccggcggc gcgatcgtgt tcgacggacg
     1501 cgatatcacg cacgtgcccc cgcacgagat cgccaagcac gggatcgcgc aaacccccgg
     1561 tgggcgcggc atcttccccg ggctcaccgt ggcggagaac ctgcagctgg ccggctggct
     1621 ctacggcgag gacaccaagc acaaggacga ggtcgagcag atcttcgggt acttcccgat
     1681 cctgcgggag cgcctgggcc agaccgcagg cagcctgtcc ggtggcgagc agcagatgct
     1741 cacgctggcg caggcattcc tcgcgcggcc caagctgctg atgatcgacg agctctcgct
     1801 cggactttcg ccggcgatcg tcgagcagct gctcggcatc gtgcgcgcga tccacgagcg
     1861 tgggacaacg atcatcatcg ttgagcagtc ggtcaacgtc gccctgacca tcgcggagcg
     1921 cgcgatcttc atggagaagg gcgaggtgcg cttcgacggt ccgacgtcgg acctgctcgg
     1981 acggcccgac atcatgcgtg cggtcttcct caagggcggg cgcagtctca gcgtcggcgc
     2041 cgggcctacc gagaccgtga agatccgcga ggaggagcgc gcgaacatcc tggaggtgcg
     2101 tggcctcgtc aagcgttacg gcggcgtcgc ggccgtggac ggggttgggt tcgtgctgcg
     2161 cgagcacgag acgctcggtc tgatcggccc caacggcgca ggcaagacga cggtgctcga
     2221 gatgatctcg ggcttcaacc ccgtggacgc aggtcaggtg atctacgacg ggcgtgacgt
     2281 gacgatgatg acgccggacg agcgcgcgaa ctacggcctg atccgtcgat tcc
//
LOCUS       DATLFO010000228         3541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_107902, whole genome shotgun sequence.
ACCESSION   DATLFO010000228 DATLFO010000000
VERSION     DATLFO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107902"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1521)
                     /gene="hflX"
                     /locus_tag="VM841_09140"
     CDS             complement(139..1521)
                     /gene="hflX"
                     /locus_tag="VM841_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HVL90385.1"
                     /translation="MPQRPTDDQEPKRRRRGGQLSDEFRAIELEREAVRLERSVLVGI
                     AYGRDTEEAEESLAELQRLAETAGSEVLDTFVQRRGTPDPATFLGKGKADEVRDSVKM
                     LGADTVIVDEELSPGQLRNLEKIVGVKVIDRTALILDIFAQHAHSVEGKAQVELAQLN
                     YLIPRLRGWGDSMSRLGGGIGTRGPGETKLEVDRRRINTRIAKLRRQIREMDKTRATK
                     RQQRERRGVPSATLVGYTNAGKSTLLNRLTQAGVLVQDKLFSTLDPTTRRLDLPDGRS
                     SVLSDTVGFVRRLPHMLVEAFKSTLEEASRADLIVHVVDGSSSDPEGQHAAVLEVLDE
                     IGAAEVPQLLVVNKTDALDEMALARLRRHWPDGVFVSAATGAGTDELLAAMAERLPRP
                     EIEVELLIPYDRGDVAAALHAAGAVVSERYADDGTIVTARLRADQLGRLDAYVLPGER
                     SRLAVPDEDL"
     gene            complement(1523..2674)
                     /locus_tag="VM841_09145"
     CDS             complement(1523..2674)
                     /locus_tag="VM841_09145"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HVL90386.1"
                     /translation="MKPARRIETLPPYLFAEIDRKVQAKRAEGVDVIGLGIGDPDKPT
                     PQHIIDAMNEAVADPATHQYPSYFGLPEFRRAVAAWYKRRFDVDLDPDTEVLPLLGSK
                     EGIAHLCTAYIDPGDVSLVPDPAYPVYDIGTRLSGGTPVPFTLSADNGFLPDYDSIQV
                     PKNTKLFWLNYPSNPMASVATLEDFAKAVDFCKANDLLFVHDNAYSEITYDGYVAPSA
                     LQAPGAKDCTIEFHSLSKTYNMTGWRIGFVVGNAAAIEALGRVKTNIDSGIFNAIQRA
                     GIAALEGPQDFLKDMIALYKTRRDRVVSTFNDAGWSLEAPKGAVYIWIPVPDGQDSAG
                     FATLLLDEAGVVVPPGRGYGEAGEGYIRISITTPDDRLDEALERIRKVL"
     gene            complement(2684..>3541)
                     /gene="dapF"
                     /locus_tag="VM841_09150"
     CDS             complement(2684..>3541)
                     /gene="dapF"
                     /locus_tag="VM841_09150"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HVL90387.1"
                     /translation="KMHGAGNDFVMVEDLQNRITPGPELVRLLCDRHFGVGADGLIRV
                     APADDADFFMDYYNADGEAAEMCGNGIRCLAKFVADRGLVGGDKLRVGTRAGIKDLEL
                     FRGPGGDVERVRVDMGPPILERKRIPLSGPGDDALRETIEVDDFTFVATALSMGNPHC
                     VLFVTDLDAVPFGYLGPTIEMLPLFPQRANIEFVQVLSRNEVRTRVWERGVGETLACG
                     TGACAIAVASALRGFTDRQVSVHLPGGTLEIDWAADGRVFMTGPAEEVFRGQLDPRFI
                     AQHLKTKTD"
BASE COUNT          556 a         1158 c         1219 g          608 t
ORIGIN      
        1 gatgatcgtt gcgggcatcg tcgccacacc caccacgtag acggcgacga tgcccgcaac
       61 gatcatccgc gagcgaatcg ttcgagcgca tccggccgct tcgctgatgc gctcgggtgt
      121 tgatccatga cgaccctgct acaggtcttc gtccggaacg gccaggcggc ttcgctctcc
      181 ggggagcacg taggcatcga gacggccgag ctggtcggcg cgcagccggg cggtcacgat
      241 ggttccgtcg tcggcgtagc gttcggatac gaccgcgccc gcggcgtgca gggctgcggc
      301 aacatcgcct cggtcgtagg gaatcagcag ctcgacctcg atctcggggc gcggcagccg
      361 ttccgccatc gccgcgagca gctcgtcggt tcccgcgccg gtcgcagccg agacgaacac
      421 gccgtcgggc caatgccgcc gcagacgcgc cagcgccatc tcgtcgagcg cgtcggtctt
      481 gttgaccacc agcagctgcg ggacctcggc cgcgccgatc tcgtccagca cctcgagcac
      541 cgcggcgtgc tggccttccg ggtcggagga ggagccgtcc acgacgtgca cgatcagatc
      601 cgcccggctg gcctcctcca aggtggactt gaacgcttcg accagcatgt gcggcagacg
      661 acgtacgaac ccgaccgtgt ccgacagcac gctcgagcgg ccgtcgggca gatccaggcg
      721 gcgagtggtc gggtcaagcg tcgagaacag cttgtcctgg accaacacgc ccgcctgggt
      781 cagccggttc agcagggtgg actttcccgc gttggtgtag ccgaccagcg tggccgacgg
      841 gacgccgcga cgctcgcgct gctggcgctt cgtcgcgcgg gtcttgtcca tctcgcggat
      901 ctggcggcga agcttcgcga tacgcgtgtt gatgcggcgg cgatccacct cgagcttggt
      961 ctcgcccgga ccgcgcgtgc cgatcccgcc gccgaggcgc gacatcgaat cgccccagcc
     1021 gcgaagccgc gggatcaggt agttgagctg tgccagctcg acctgcgctt tgccttcgac
     1081 cgagtgcgcg tgctgcgcga agatgtcgag gatcaacgcg gttcggtcga tcaccttgac
     1141 gccgacgatc ttctcgaggt ttcgaagctg gccgggtgag agctcctcgt ccacgatcac
     1201 ggtgtcggca ccgagcatct tgacgctgtc gcggacctcg tcggcctttc ctttgccgag
     1261 aaaggttgcg gggtcgggcg tgccgcggcg ctgcacgaac gtgtcgagga cttccgaccc
     1321 ggcggtctcg gcgaggcgtt gcagctcggc gagcgactcc tcggcttcct cggtgtcgcg
     1381 accgtaggcg atgccgacga gcaccgagcg ttccaggcga acggcttcgc gttcgagttc
     1441 gatggctcga aactcgtcgc tgagctgtcc gccgcggcgg cggcgtttcg gttcctggtc
     1501 gtcggtgggg cgttgcggca cgctacagaa cctttcggat gcgttcgagc gcttcgtcca
     1561 agcgatcgtc gggcgtggtg atcgagatac ggatataccc ttccccggct tctccgtatc
     1621 cgcggcccgg cggcacgacg acaccggcct cgtccagcag cagcgtcgcg aacccggccg
     1681 agtcctgccc gtcgggaacc gggatccaga tgtagacggc acccttggga gcctccagcg
     1741 accatcccgc gtcgttgaag gtggacacga cgcgatcgcg gcgggtcttg tagagcgcga
     1801 tcatgtcctt caagaaatcc tgcggccctt cgagcgccgc gatacccgcg cgctggatcg
     1861 cgttgaagat gccggagtcg atgttggtct tgacgcggcc gagcgcctcg atggccgcgg
     1921 cgttgccgac gacgaacccg atccgccagc cggtcatgtt gtaggtcttc gacagtgagt
     1981 ggaactcgat cgtgcagtcc ttggctccgg gagcctggag ggcactcggc gcgacgtagc
     2041 cgtcgtaggt gatctcggag taggcgttgt cgtggacgaa tagcagatcg ttcgccttgc
     2101 agaagtccac ggctttcgcg aagtcctcga gcgttgcgac cgaggccatc gggttcgacg
     2161 gatagttcag ccagaacagc ttcgtgttct tcggaacttg aatcgagtcg tagtcgggca
     2221 ggaagccgtt gtcggcgctg agcgtgaacg ggaccggcgt gccgcccgac aggcgcgtgc
     2281 cgatgtcgta gacgggatac gcggggtcgg gcaccagcga cacgtcgccc ggatcgatgt
     2341 aggcggtgca caaatgcgcg atgccttctt tcgatccgag gaggggaagc acctcggtgt
     2401 cgggatccag gtccacgtcg aaacggcgct tgtaccaggc cgcgaccgcg cggcgaaact
     2461 cgggcaggcc gaagtaggag ggatactggt gcgtggcggg atcggcgacc gcttcgttca
     2521 tcgcgtcgat gatgtgctga ggggtcggct tgtcgggatc gccgatgccc agcccgatca
     2581 cgtccacgcc ttcggcgcgc ttggcctgta ccttgcggtc gatctcggcg aacaggtacg
     2641 ggggcagcgt ctcgatacgg cgagcaggct tcacgcgatc ctcctagtcg gtcttcgtct
     2701 tgaggtgctg tgcgatgaac cgcggatcga gctgcccgcg gaagacttcc tcggccggac
     2761 cggtcatgaa tacgcggccg tccgcggccc aatcgatctc gagggtccca cccggcaggt
     2821 gcaccgatac ctggcggtcg gtgaagccgc ggagcgcgga ggccaccgcg atcgcgcagg
     2881 cgccggtccc gcaggcgagc gtctcgccga cgccgcgctc ccacacgcgc gtgcgcacct
     2941 cgttgcgcga aaggacctgg acgaactcga tgttggcgcg ctgcgggaac agcggaagca
     3001 tttcgatagt ggggccgaga tacccaaagg gaaccgcatc caggtcggtg acgaacagaa
     3061 cgcagtgcgg gttgcccatc gacagcgccg tcgcgacgaa ggtgaagtcg tccacctcga
     3121 tggtctcgcg cagcgcgtcg tcgccggggc ccgacagcgg gatgcgctta cgttccagga
     3181 tcggcgggcc catgtccacg cgaacgcgtt cgacgtcgcc gccgggtccc cggaacagct
     3241 cgaggtcctt gatgcccgcg cgcgttccca cgcgaagctt gtcgccgccg accaggccgc
     3301 ggtcggcgac gaacttcgcg agacagcgga tgccgttgcc gcacatctcg gcggcctcgc
     3361 cgtcggcgtt gtagtagtcc atgaagaaat ccgcgtcgtc ggcaggcgcg acccggatca
     3421 gcccgtccgc tccgacgccg aaatgccggt cgcacagcag gcgcaccagc tcggggccgg
     3481 gcgtgatccg gttctggaga tcctcgacca tcacgaaatc gttgccggcg ccgtgcatct
     3541 t
//
LOCUS       DATLFO010000229        16871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_4629, whole genome shotgun sequence.
ACCESSION   DATLFO010000229 DATLFO010000000
VERSION     DATLFO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16871
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4629"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VM841_09155"
     CDS             complement(<1..340)
                     /locus_tag="VM841_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90388.1"
                     /translation="MSTADVRPLRRRIAERYAGRRGRNAILAVGLMLVMMTGGLLLLR
                     GYQARPLLFNDDGVTVKQRALHCPAAWKAVGAKFNKADPRTPDDFRTCVETGRMKAFT
                     AFLISSFIGVA"
     gene            524..1387
                     /locus_tag="VM841_09160"
     CDS             524..1387
                     /locus_tag="VM841_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HVL90389.1"
                     /translation="MNKNIMKTFVFMAALGGLFVIVGGSLGGRSGMVIALAFAVLMNM
                     GAYWFSDTLVVKMTRSKPVSEKDAPWLYRIVQPLAQKAGMPMPKLYLMPSAQPNAFAT
                     GRNPDRAVVAVTEGILNVLSERELEGVLAHELAHVKNRDILIGAVAATVAAAITMLAR
                     LAMWASILGGGGRDRESNSPFGAVGMLLSFILAPIAAMMIQMAVSRSRESQADRTGAE
                     LLGDATPLANALIKIDAAAKKMPPMEVNPAVSHVFLQSPFRGQNMTKLFMSHPPLEER
                     IAQLREMGAQV"
     gene            1601..2758
                     /locus_tag="VM841_09165"
     CDS             1601..2758
                     /locus_tag="VM841_09165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVL90390.1"
                     /translation="MKTPSSLRARTVLAAATVLALLALVAPARADDPIPPILPTGSPS
                     PSPSPSGSAKPKPKPKPSPSSTPSSTPRPAPKGPAPDPYRGAIAEAVDYWRNLPKTPA
                     RTTTALLELLQRQLPPGHELDRSTLVKGVWRFPIAGYTWYQDDFAAPRYEPYFHMHEG
                     TDLFAQEGTLVVASADGVITRLLAGSIGGNGIWLTEADGTYYYYGHLKSYAPGLTQGM
                     RVKIGQVLGTVGRTGIAVETYPHVHFEVHPGGGKAVNPKPFLDRWLFEAEARAQEALR
                     RQAALTAAARIGAARWGTLFDLLARPAAEPAGWWASALDGATLGFADLALDRIAYEVD
                     WASVADGTGSHEHEEASPVTPALIDGGILAHGGRPGTFDDVYRSRAASASA"
     gene            complement(2725..3591)
                     /locus_tag="VM841_09170"
     CDS             complement(2725..3591)
                     /locus_tag="VM841_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90391.1"
                     /translation="MTETVGLVTCAEFPDLTPDDRLLIEPLRSHGLQARPISWDAPTE
                     WDSFDALVIRSTWDYFERPDEFAAWIDERAAAGPPLFNPPEVLRWNMHKHYLRELRAA
                     GVDTVETHWIERGSDADLAGVLHERRWEHAVVKPAVSAGAWKTSPIRRDDLAPHDAML
                     RDLIAGCDVMVQPFLNQIVDEGEWSVIFYGGEHASTLLKRPAGGDYRVQERHGGTTTY
                     FADPPPGLVEQAAAVLEHAPGDLLYARVDGVLDGNRFVLIELEVLEPYLFFGATPGGA
                     ERYADALAARLR"
     gene            3696..4010
                     /locus_tag="VM841_09175"
     CDS             3696..4010
                     /locus_tag="VM841_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90392.1"
                     /translation="MKGPSFASVMAAAATVAGASFVAVGATKLDNQTSKAILKERGLP
                     AQYELIEQARRFDICVERVDATTLRVTGPGGPEAAKARNPELYEQVAQAVDVINWYEI
                     RC"
     gene            4012..4854
                     /locus_tag="VM841_09180"
     CDS             4012..4854
                     /locus_tag="VM841_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL90393.1"
                     /translation="MSWFRRKKDKPARPVADFDWRSYDSVAEDYARIHGAHTAIVAAD
                     VLEGVGAGPGRRILDVGTGTGDALTVAEQADPGGVVVGIDSAPGMLRLAKAQHFVAAA
                     EVINLPFNDGTFDGVMANFALPYFTKLDTALFDVRRVLKPGGMLGVAVWAAGEDDLTK
                     TWRVLVEEAIGRDILKAGLKDEAPWAEKLADKHRLETVLRDAGFRPVTVEAKRYKFAT
                     TREEYIRGHEIEAAGRYTKVMLGERLWPNFQKRVRAAYEERFPASLVDFRDVLLAVAT
                     KPPR"
     gene            complement(4851..6227)
                     /locus_tag="VM841_09185"
     CDS             complement(4851..6227)
                     /locus_tag="VM841_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL90394.1"
                     /translation="MRPRRRLLVAVFVGFALALLAGTLIPMGLLSARSYRNSYLSQRV
                     RETDVVVDSLRGRSNEERARVIRDRYPAAAFTTGWLLDPNLASVAEPSQPNAFGLPPS
                     TAPEVRRALLGPDARRFGSSPIGERLYIAVPLIEDGALRNVLWVSTSLRPVGDLTQRS
                     WVLLGGIGFAALVLAVALGVALASRLSRRIEVLAAGAARFGEGRLNEPIVVAGRDEVA
                     ALATRLNEMAAGLAGSMDREKQFVTAASHQIRTPLTSIRLRLEAIAERLKRVDEDTRE
                     YVGEMIEEIDRLTDLSSKLLALGAGWSGLGEAGPVELDVAMRETVARLEPVARHRGVP
                     LDVRAASTPLSLRAVPGAFEEVVLNLLDNALKFSPSGEPVCVSWREHIGRARIEIADH
                     GPGISEAERSRVFEPFYSSRRREGGHGLGLTISAQLCEMSNATLRFEQNEGGGTLAVL
                     DWPLEPGS"
     gene            complement(6224..6901)
                     /locus_tag="VM841_09190"
     CDS             complement(6224..6901)
                     /locus_tag="VM841_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90395.1"
                     /translation="MRVLLVEDEPMISKALRRELERVGFEVNGCDSGEAALRLAESWS
                     PDVMLLDLMLPDLDGMEVARRVRDVSDTPIIMVTARGQEAERIAGLELGADDYIVKPF
                     SIPELIARMRAVRRRSTGVPKRETTLRHRDIEMDLDRHRVTKDGVELDLTNKEFEILR
                     MLLARPGGIVRRADIIHLIWGLNLDDGANTLDVHVSVLRKKIGDDARSPRYIETVRSV
                     GFRLVEA"
     gene            7047..8744
                     /locus_tag="VM841_09195"
     CDS             7047..8744
                     /locus_tag="VM841_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90396.1"
                     /translation="MSRRLFVPLVLALLAASFPAAADHDAAVSPPDPRHRLNAMVPKA
                     PGTVETIVLQYGPYVVHPGSDLTRVDAEPVLRDAFVIAARPAVRLADGTEPHDDVVHI
                     HHAHWIKPDPNQSSGYRWFFGTGEEKTRGGGWQYMRADVPRWNAGVRYGVPMERGEQM
                     VFLSMLHNKTAEPLTLWIEGRFEIVWGSRDQIRAVTAADPDHPLWDAGGGSPYAAAGI
                     DMRPLTPVLHGRTYQVPRTGGMHVWPRDAAGAPGTGDGVQPGLGDVWVAPADGTIVIG
                     AGHLHPGGRRVVVSNLGSPASPCGSSYGHDADGDGFAGRAILDISAYYQPDENGNEWF
                     PSEEFQMGITQSGWRAEVRAGDRIAINGIYDATDYAYPDAMSFFGFYMDTGAGRAQGS
                     PCTSWLADNPSAPQSAVLPSTPNRPWEEHTEVCDPCDDPASAVTELGPWTPVVQIAGF
                     AYLPGNLGSTEATGVPWVRRGETLTFVNEDWAAGLVRHSVTSCRWPCNGPYKANYPFH
                     DGVLDSGSLGFTPVDTYDTGSDIPEWRLDTSAIGPGTYSYFCRLHPSMRGSFAVVAA"
     gene            8944..9120
                     /locus_tag="VM841_09200"
     CDS             8944..9120
                     /locus_tag="VM841_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90397.1"
                     /translation="MPSTAGTVSAAAPDGTSYSGTMTDVLVASPTSGLWNFFRTGQSS
                     AVGLYLVTVPGHSW"
     gene            complement(9136..9630)
                     /locus_tag="VM841_09205"
     CDS             complement(9136..9630)
                     /locus_tag="VM841_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HVL90398.1"
                     /translation="MAKDSSFDVVSEVDRQEVDNALNQAGKELSQRYDFKGTGTSINW
                     QGDLEIRITSSAEQRVQAALDVFQEKCVKRGISLKALQLEPIKEAGGGTYKMDIKINQ
                     GIGEDKAKAIVKSLKQSGLKVQAAIQGEQVRVSGKTRDVLQEAIAHLKEADFGIPLQF
                     TNYR"
     gene            9703..9787
                     /locus_tag="VM841_09210"
     tRNA            9703..9787
                     /locus_tag="VM841_09210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9737..9739,aa:Tyr,seq:gta)
     gene            9895..9970
                     /locus_tag="VM841_09215"
     tRNA            9895..9970
                     /locus_tag="VM841_09215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9928..9930,aa:Thr,seq:ggt)
     gene            10005..10079
                     /locus_tag="VM841_09220"
     tRNA            10005..10079
                     /locus_tag="VM841_09220"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10037..10039,aa:Met,seq:cat)
     gene            10262..11746
                     /locus_tag="VM841_09225"
     CDS             10262..11746
                     /locus_tag="VM841_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HVL90399.1"
                     /translation="MLKRTLLIAAITAMLLSGAAVRADAAPAPWPGVKLLTTEDFVEV
                     VRWPDEPFIGDLGIVVAAGNRPFELHLSKSNYRAPIRVTQHDRTGRRSRFLAEATKAS
                     FAEGLKDFFIVTVTRPDGTRVLRRDAGFCTDAWDRMRLTISAPETSRYPNGCSDIPYA
                     KGTVLGIEAGWGMPLRTMPALNVPDGEYTVTISVRYGYRRLFSISEQDAVASVDVSLV
                     TVDECCVEPPPEMRARRRAAAAAPLRPVRPSGAPAAAPATDTRPDLVAIPAWNIRIGN
                     PDGHVDEPQPEPEGRDILRFSSTVWNAGPAPLVVDGFRNKGEPEMTGYQSFYKNGKRV
                     GWKQIGAFEFDDRDGHQHWHFKDFAQYRLLDAGKMDVLPAGKEAFCLAPTNAIDMTVP
                     GADWSVHRVEFPGSMCGQRSSLAIREVLQAGWADTYGTGTPGQEFDVTDLPNGTYFIE
                     VAANPLGRLIERVTSNNVALREIVLGGTPGNRTVVVRPYQGIAA"
     gene            11869..12033
                     /gene="rpmG"
                     /locus_tag="VM841_09230"
     CDS             11869..12033
                     /gene="rpmG"
                     /locus_tag="VM841_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007053013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HVL90400.1"
                     /translation="MAKSDKRPGITLACEQCKRRNYQTTKNKVNTRDRLQLKKYCAWC
                     GSHTDHRETR"
     gene            12208..13014
                     /locus_tag="VM841_09235"
     CDS             12208..13014
                     /locus_tag="VM841_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL90401.1"
                     /translation="MGRLDDRVAIITGGGRGIGRATALRFAAEGAAVVVNDVDADPAE
                     ETAEIIRKEGGKALVSLHNTVEAANAEAMVKAALDEFGKLDILVNNAGITRDKTFHNM
                     TPDIWDLGIKVNLYTAYHSTFYAVQHMRSAAKKELEEKGAVSYHRKITFTASSVALTG
                     NPGQANYTAAKGALISLTKTLARELGPFKINCNAVAPGFVETRLTAAKEEGSDLGIPD
                     QLRNMALMMIALGRAGLPEDLANVHLFLCSQDSDFVSGVTIPVTGAQLGA"
     gene            13029..13487
                     /locus_tag="VM841_09240"
     CDS             13029..13487
                     /locus_tag="VM841_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HVL90402.1"
                     /translation="MPLNTALVGKEYPKSPVYEVGREKIREFAMAIGDPNPLYTDESA
                     AKAAGYADIIAPPTFLTIIGFKFGPSIVLDPELGLDYTRVVHGEQEFELKRPVVPGDR
                     LTGTPKIVDISAKGKNEFLTYEAVITAESGEEVAIARSTIVSRGTAPQEG"
     gene            13493..13918
                     /locus_tag="VM841_09245"
     CDS             13493..13918
                     /locus_tag="VM841_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HVL90403.1"
                     /translation="MTAKVNYADVSEGEELKPWTYNVQRMNLVMYAGASGDFNIIHWN
                     EEIAKMVGLPNVISHGMFTMAKVGQYVTDWAGDAGAVVRFKTRFTKPVVVDNQAGNNV
                     TVTGTVKQKLDGNRVLIELSATTQEGDNCAAAEAEVVLA"
     gene            14118..14837
                     /locus_tag="VM841_09250"
     CDS             14118..14837
                     /locus_tag="VM841_09250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL90404.1"
                     /translation="MLDRPAACAISIHVLYEGFRNDLSRFVDSVARHGAGHDYEIVIT
                     ECASPEGASSDGEWIESLAGERVRVLHFADDPGWAAGRNAAIGVSRGALIAVADLSVE
                     LTGDLLGPLVKIFDDGRVGVAGPWGLRTRNLRDFEEVEGPEVAHAIQGYFLTTRRDIA
                     REHRFDRKFAWYRHADIDLSFAIRAAGYRAVATEPAATRHTHRAWMALDEDERAKRSR
                     RNFSRFLDRFKDRTDLIETGV"
     gene            14877..15440
                     /locus_tag="VM841_09255"
     CDS             14877..15440
                     /locus_tag="VM841_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL90405.1"
                     /translation="MEIPPDLVEACRRGDVKAFEALVRATERQVYTVVYRIVGNHDDA
                     ADVAQEVYLKVWKAIRQFRGDAAVTSWLYRVATNAAIASLRKRGRLADPVEPDRLAEI
                     EAPAVTPAVDTGDVEQALTRLPAAYRTVLVMRETYGMSIEEIAKQMGSSTGAAKVRLH
                     RARLRLADELERAGVVVPLQRRGKKTS"
     gene            15437..15838
                     /locus_tag="VM841_09260"
     CDS             15437..15838
                     /locus_tag="VM841_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVL90406.1"
                     /translation="MNCERVRPYLTAHAAGELPAHTASWVNPHVEDCPVCSSLSLRHA
                     TIRVALRTLPEATVDPPPGFAATVARRAARLKRARPIPIPPMIPPDAIRVLTDNRDAI
                     LGSAGVALGAAGVAWIAWRSMRSRKPGPRTA"
     gene            15936..16012
                     /locus_tag="VM841_09265"
     tRNA            15936..16012
                     /locus_tag="VM841_09265"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15970..15972,aa:Trp,seq:cca)
     gene            16104..16409
                     /gene="secE"
                     /locus_tag="VM841_09270"
     CDS             16104..16409
                     /gene="secE"
                     /locus_tag="VM841_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HVL90407.1"
                     /translation="MVNRATKRLLAKQQKKEQGGKPRTREEAVAERRSAIERKKRTPP
                     RQFLREVRGELKKVAWPSRREVSTYTVVVLVSVIFITGIVFALDYGFNQAIFQTFSK"
     gene            16436..>16871
                     /locus_tag="VM841_09275"
     CDS             16436..>16871
                     /locus_tag="VM841_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90408.1"
                     /translation="MDDSSVNGVRADRENDDEPQPPSGNGHGNGSGDDTAQDAPEAQA
                     GTAAIDIGDVPVDAADVADAMATSGEHLTSEADPATIEEQSGAFEQAEEPQAEEPAVE
                     SNGHAAPAEPAAAAEPEPKAEPEPKAEPEPAKPKGAKGKDDGG"
BASE COUNT         2806 a         5785 c         5697 g         2583 t
ORIGIN      
        1 cggcgacgcc gatgaacgat gagatcagga acgcggtgaa ggccttcatg cgtccggttt
       61 cgacgcatgt gcgaaagtcg tcgggcgtgc gtgggtcggc cttgttgaat ttcgccccga
      121 cggccttcca agccgccggg cagtgcagcg cacgctgctt gacggtcacg ccgtcgtcgt
      181 tgaacagcag cggccgtgcc tggtagccgc gcagcagcag caagccgccg gtcatcatga
      241 ccagcatcaa gccgaccgcc aggatcgcgt tgcggccacg gcggcccgcg taacgttccg
      301 cgattcgcct gcgcagcgga cgcacgtctg cggtgctcat gcgggccagc atgaaccacc
      361 gcggcgcatc gggcaaccga tatttcggac ggatttcccc gcaggcaggc cccggacctg
      421 ggcgttcgct cgctctcagc agtggcttag caggccggat accatttcga ggtgagtcac
      481 gccgtccgga ggctccggcg cggggctcag gaggttgggt tcgatgaaca aaaacatcat
      541 gaagacgttc gtgttcatgg ccgcgctcgg cggtctgttc gtgatcgtcg gcgggtcgct
      601 cggtggccgg agcggcatgg ttatcgcgct cgcattcgcg gtcctgatga acatgggcgc
      661 gtactggttc agcgacacgc tcgtggtgaa gatgacgcgc agcaagccgg tgagcgagaa
      721 ggatgcgccg tggctctacc ggatcgtgca gccgctcgcg cagaaggccg gcatgccgat
      781 gccgaagctg tacctgatgc cgtcggcgca gccgaatgcc ttcgccaccg gacgtaatcc
      841 cgaccgcgcg gtcgtcgcgg tcaccgaagg aatcttgaat gttctgtcgg aacgcgagct
      901 ggaaggcgtt ctggcgcacg agctggcgca cgtgaagaac cgcgacatcc tgatcggcgc
      961 cgtcgccgcg acggtcgccg cggcgatcac gatgctcgca cggctggcga tgtgggcctc
     1021 gatcctgggc ggcggcggac gcgaccgcga aagcaacagc ccgttcggcg cggtcggcat
     1081 gctgctgtcg ttcatcctgg ctccgatcgc ggcgatgatg attcagatgg ccgtgtcgcg
     1141 ctcgcgcgag tcgcaggcgg accggaccgg tgccgagctg ctcggcgacg ccacgccgct
     1201 ggcgaacgcg ctgatcaaga ttgacgcggc cgccaagaag atgccgccga tggaggtcaa
     1261 ccctgcggtg tcgcacgtgt tcctgcagag cccgttccgc ggccagaaca tgacgaaact
     1321 gttcatgagc cacccgccgc tcgaggagcg catcgcacag ctgcgcgaga tgggagcgca
     1381 ggtctaagcg agcaagcctt ccgtcaggaa cgaccggcct tcgggccggt tgttccgttt
     1441 ttgcggcggt ttaacgtatc ccttcggttg ggtcgctcgt tgtgctgggt agaccgcggc
     1501 aggattttcg ggcccgaagg aagaaaccac gaagcgtcca gggaaaacgc gaggccgtgt
     1561 ccggcgacga ccggacgccc cgtacggccg tccgacatag atgaagacac cgagctcgct
     1621 tcgcgcgcgg acggtccttg cagcagcgac cgtcttggcg ctgctcgccc tggtcgcgcc
     1681 cgctcgcgcg gacgacccca ttccgccgat cttgcccacc ggctccccgt ccccgtcgcc
     1741 gtcgccgtcg ggcagcgcca agcccaagcc caagccgaaa ccctccccct cctcgacgcc
     1801 gtcatcgacg ccgcgtccgg cgccaaaagg ccccgcgccc gatccgtacc gcggcgcgat
     1861 cgccgaggcg gtcgactatt ggcggaacct gcccaagacg cccgcgcgga ccacgacggc
     1921 gctgctcgag cttcttcagc gccagctccc gcccggacac gagctcgacc gctcgacgct
     1981 ggtcaaggga gtctggcgat tcccgatcgc cggctacacc tggtatcagg acgacttcgc
     2041 ggccccgcgt tacgagccgt acttccacat gcacgaggga acggatctgt tcgcccagga
     2101 aggcacgctc gtggtcgcat cggccgacgg cgtgatcacg cgcctgctcg ccgggtcgat
     2161 cggcggcaac ggcatctggc tcaccgaggc cgacggaacc tactactact acggccacct
     2221 caagagctac gcgcccggtc tgacccaagg catgcgcgtc aagatcggcc aggtgctcgg
     2281 aaccgtcggc cggaccggca tcgccgtcga gacctatccg cacgttcact tcgaggtgca
     2341 ccccggaggg ggcaaggccg tcaaccccaa gcccttcctg gaccgctggc tcttcgaagc
     2401 ggaggcgcgc gcgcaagagg cgctgcgccg ccaggcggcg ctgaccgccg cggcacggat
     2461 cggcgcggcc cgctggggca cgctcttcga tctgctcgcg cgcccggccg ccgagccggc
     2521 gggctggtgg gcctccgcgc tcgacggcgc gacgctcggg ttcgccgatc tcgccttgga
     2581 tcgcatcgca tacgaggtgg attgggcatc cgtcgcggat ggaaccggat cgcacgagca
     2641 cgaggaggca tcgcccgtca cgccggcgtt gatcgacggc gggatcctcg cgcacggcgg
     2701 ccggcccggg acgttcgacg acgtctatcg cagccgcgcc gcgagcgcgt cggcgtagcg
     2761 ctccgcacca ccgggtgtcg ctccgaagaa cagatacggc tcgagcacct cgagctcgat
     2821 gagcacgaaa cgattcccgt cgagcacgcc gtcgacgcgc gcgtacagca ggtcgcccgg
     2881 agcgtgctcg agtacggccg cggcctgctc gaccagtccc ggcggcgggt ccgcgaaata
     2941 ggtcgtcgtg ccgccgtggc gctcttggac tcggtagtcg ccgccggcgg gacgcttcag
     3001 cagcgtcgag gcatgctcgc cgccgtagaa gatcaccgac cattcgcctt cgtccacgat
     3061 ctggttcagg aacggctgga ccatcacgtc gcagccggcg atcaggtcgc gcagcatcgc
     3121 gtcgtgcggc gccagatcgt cgcggcggat gggtgaggtc ttccatgcgc cggccgagac
     3181 ggcaggcttg acgacggcgt gctcccaccg ccgctcgtgg agaacgccgg cgaggtcggc
     3241 gtcggagccg cgctcgatcc aatgggtctc gaccgtgtcc acccctgcgg ctcggagctc
     3301 gcgcaggtag tgcttgtgca tgttccaccg caggacctct ggcggattga acagcggcgg
     3361 tccggcggct gcgcgctcgt cgatccacgc ggcgaactcg tccggccgtt cgaagtaatc
     3421 ccacgtcgag cggatcacga gcgcgtcgaa ggagtcccat tccgtcgggg catcccagct
     3481 gatcggacgc gcctggaggc cgtgcgagcg cagcggctcg atcaggagcc ggtcgtcggg
     3541 agtgaggtcc ggaaactcgg cgcaggtgac cagtcccacc gtctccgtca tccgttcctc
     3601 cgatgtgccc gctccgctcg aagcgcgtag cctatcggcc acgcgggccg tgcgctccgc
     3661 cattccgtat cgagagaagg aaaggatcga cgtcgatgaa gggcccgagc ttcgcctccg
     3721 tcatggccgc cgccgcgacc gtcgccggtg cctcgttcgt cgcggtggga gcgacgaagc
     3781 tggacaacca gacctcgaag gcgatcctga aagaacgcgg cctgcccgcg cagtacgagc
     3841 tgatcgagca ggctcgccgg ttcgacatct gcgtcgagcg ggtcgatgcg acgacgctgc
     3901 gcgtcaccgg ccccggcgga ccggaggccg ccaaggcgcg caacccggag ctctacgaac
     3961 aggtcgcgca agcggtggac gtgatcaact ggtacgagat tcgctgctga catgtcctgg
     4021 ttccggcgaa agaaggacaa gcccgcccgc ccggtcgcgg atttcgactg gcggtcctac
     4081 gattcggtcg ccgaggatta cgcgcgcatc cacggcgcgc acaccgcgat cgtcgccgcc
     4141 gacgtgctcg agggcgtcgg tgcggggccg ggcaggcgca tcctcgacgt cggtaccgga
     4201 accggcgacg cgctgaccgt cgccgagcag gccgacccgg gcggcgtcgt ggtcgggatc
     4261 gactcggctc ccggcatgct gcgtctggcg aaggcgcagc acttcgtcgc cgccgccgag
     4321 gtcatcaacc ttccgttcaa cgacggcacc ttcgacggcg tcatggcgaa cttcgcgctg
     4381 ccgtacttca cgaagctcga cacggcgctg ttcgacgttc gccgcgtgct caagcccggc
     4441 gggatgctcg gcgtggccgt gtgggccgcc ggggaggacg acctgaccaa gacctggcgc
     4501 gtgctcgtcg aagaggcgat cggccgcgac atcttgaagg ccgggctcaa ggacgaagcg
     4561 ccctgggcgg agaagctcgc cgacaagcac cggctcgaga cggtactgcg cgacgcgggg
     4621 tttcggccgg tcaccgtcga ggcgaagcgc tacaagttcg cgacgacgcg cgaggagtac
     4681 atccgtggcc acgagatcga agccgcgggg cgatacacga aggtgatgct gggcgaacgt
     4741 ctgtggccga acttccagaa gcgcgtgcgc gccgcctatg aggagaggtt tcccgcctcg
     4801 ctcgtcgact tccgcgacgt tctgctcgcg gtcgccacca aaccgccgcg ttagctcccc
     4861 ggctcgagcg gccagtcgag cacggccagt gtgccgccgc cctcgttctg ttcgaaccgc
     4921 agcgtggcgt tggacatctc gcacagctgc gccgagatgg tcaggccgag cccatggccg
     4981 ccctcacgcc gtcgcgacga gtagaagggc tcgaagaccc gcgaccgttc cgcttcgctg
     5041 attccgggcc cgtgatcggc gatctcgatg cgggcgcggc cgatgtgctc gcgccaggac
     5101 acgcacaccg gctcgccgga gggggagaac ttgagcgcgt tgtcgagcag gttgagcacg
     5161 acctcctcga atgcgccggg aaccgcgcgc agggacaacg gcgtcgaggc ggcacgcacg
     5221 tccagcggca ccccccggtg gcgagcgacc ggctcgagcc gcgcgacggt ctcgcgcatc
     5281 gcgacgtcga gctcgacggg gccggcttcc cccaggccgc tccagccggc gccgagcgcc
     5341 agcagcttcg aggacagatc ggtgaggcgg tcgatctctt cgatcatctc tccgacgtac
     5401 tcgcgcgtgt cctcgtccac ccgcttgagg cgttcggcga tcgcctcgag ccgcaggcgg
     5461 atcgaggtga gcggcgtgcg aatctgatgc gaggcggcgg tcacgaactg cttctcgcga
     5521 tccatgcttc ccgcgaggcc ggcggccatc tcgttgaggc gggtggcgag cgcggcaacc
     5581 tcgtcgcgcc ctgcgacgac gatcggctcg ttgagccggc cttcgccgaa gcgggcggcg
     5641 ccggctgcga gcacctcgat ccggcgggaa agccgggacg cgagcgcgac gccgagcgca
     5701 acggcgagca cgagcgcggc gaagccgata ccgccgagca gcacccagct tcgctgggtc
     5761 agatcgccga ccggccgaag cgatgtcgag acccacagca cgttgcggag cgcgccgtcc
     5821 tcgatgagcg gcaccgcgat gtagaggcgc tcgccgatcg gggacgagcc gaagcggcgc
     5881 gcgtcgggac cgagcagcgc gcgtcgaacc tcgggagccg tgctcggcgg cagcccgaag
     5941 gcgttcggct gcgacggctc ggcgacgctc gccaggttcg gatccagcag ccacccggtc
     6001 gtgaaggccg cggcgggata tcgatcgcga atgacccggg cccgctcctc gttgctgcgc
     6061 ccgcggagag aatcgacgac gacgtcggtc tcgcgcacgc gctgtgacaa gtaggagttg
     6121 cgataggacc gggccgacag caggcccatc gggatcagcg tccccgccag cagcgccagc
     6181 gcgaatccca cgaagacggc gaccagcagt cgccggcggg gtctcatgcc tcgacgaggc
     6241 gaaaaccgac ggagcgaacc gtctcgatgt accggggtga ccgggcgtcg tccccgatct
     6301 tcttgcgcaa cacgctcacg tgcacgtcga gcgtgttcgc gccgtcgtcg aggttgagcc
     6361 cccagatcaa atggatgatg tccgcacgcc gcacgatgcc gccgggacgc gccagcagca
     6421 ttcggaggat ctcgaactcc ttgttcgtga ggtccagctc gacgccgtcc ttcgtgaccc
     6481 ggtgccggtc cagatccatc tcgatgtcgc ggtgccgcag cgtggtttca cgcttcggaa
     6541 cgcccgtcga gcgacggcgc accgcgcgca tccgcgcgat cagctcgggg atgctgaagg
     6601 gcttgacgat gtagtcgtcc gcgcccagct cgaggccggc gatgcgctcg gcttcctggc
     6661 cacgggccgt gaccatgatg atgggggtgt cggacacgtc gcggacccgc cgcgcgacct
     6721 ccatgccgtc caggtccggc agcatcaggt cgagcagcat cacgtcggga ctccagcttt
     6781 cggccagccg cagggccgcc tcgcctgagt cgcagccgtt aacctcgaag ccgacgcgct
     6841 ccagctcgcg ccgcagcgct ttggagatca tcggctcgtc ctcgaccagc aagactctca
     6901 tgatccgaca attgtgcctc caatcggccg cgaaagacag tcgggacggc cgcgtgagcg
     6961 gggagggacg agcccggcca ttgtcttttc ggcgcccaag gcgtacatat ggttcggatc
     7021 ggttcgtaac ggcccaagga ggaaccatgt cgcgccgcct cttcgttccg ctcgtgctcg
     7081 ctctgctggc ggcctcgttt cccgcggccg ccgaccacga cgccgccgtg agcccgcctg
     7141 atccgcgaca ccggctcaac gccatggtcc cgaaagctcc aggcaccgtc gagacgatcg
     7201 tgcttcagta cggcccctac gtcgtgcacc ccggctccga tctgacgagg gtcgacgccg
     7261 agcccgtcct ccgcgacgcg ttcgtgatcg cggcccgccc ggccgtgcgc ctggccgacg
     7321 gaaccgagcc ccacgacgac gtggtgcaca tccatcacgc ccactggatc aagccggacc
     7381 cgaaccagag cagcggttac cggtggttct tcggcaccgg cgaggagaag acccgcggcg
     7441 gcgggtggca gtacatgcgc gccgacgtgc cgcggtggaa cgccggcgtg cgctacggcg
     7501 tgccgatgga gcgcggcgag cagatggtct tcctttcgat gctgcacaac aagaccgcgg
     7561 agccgctgac gctatggatc gagggccgct tcgagatcgt gtggggatcg cgcgatcaga
     7621 tccgggccgt gaccgcggcc gatcccgatc atccgctctg ggacgcgggc ggcggaagcc
     7681 cctacgccgc tgccggcatc gacatgcgcc ccctcacccc cgtgcttcac ggacgcacct
     7741 atcaggtgcc ccgcaccggc ggcatgcacg tgtggccgcg cgacgcggcg ggcgcgccgg
     7801 gcacgggcga cggcgtccag cccggcctgg gcgacgtgtg ggtcgcgccg gcggacggca
     7861 ctatcgtgat cggtgccggt caccttcatc ccggaggccg ccgcgtggtc gtgtccaacc
     7921 tcggcagccc ggccagcccg tgtggctcgt cctacggcca cgatgccgac ggggacggct
     7981 tcgccggccg ggccatcctc gacatcagcg cctactacca gccggacgag aacggaaacg
     8041 aatggttccc gtcggaagag ttccagatgg gcatcaccca gagcggatgg cgcgccgagg
     8101 tccgcgcggg cgatcgcatc gcgatcaacg gcatctacga cgcgaccgac tacgcctatc
     8161 ccgacgccat gagcttcttc ggcttctaca tggacaccgg cgccggccgc gcgcagggct
     8221 ctccgtgcac ctcgtggctg gccgacaacc cctccgcgcc ccaaagcgcc gtgctgccga
     8281 gcacgccgaa ccgtccgtgg gaggagcaca ccgaggtgtg cgatccgtgc gacgacccgg
     8341 cgtcggcggt caccgagctc ggcccgtgga cgccggtggt tcagatcgcc ggcttcgcgt
     8401 acctgcccgg caacctcggc tcgaccgagg cgaccggcgt gccgtgggtc cgccgcggcg
     8461 agacgctcac gttcgtcaac gaagactggg ccgccggatt ggtgcgccat tcggtgacgt
     8521 cgtgccggtg gccgtgcaac ggaccgtaca aggcgaacta tccgttccac gacggggtgc
     8581 tcgactccgg gtcgctcggc ttcacgccgg tggataccta cgacacgggc tcggacatcc
     8641 ccgaatggcg tctggacacc tcggcgatcg gccccggcac ctacagctac ttctgccgct
     8701 tgcacccgtc gatgcggggc tcgttcgcgg tcgtcgccgc gtagccgctc agcgatcgtt
     8761 cgtcacctga cgatcatttt acgggcgttc tggcgatggt cttcgagcta tgacttcacg
     8821 ggaagcaatg ctcgatacgt cccggccacc cagttctccg gcgtgagcgc catggcggat
     8881 agcgagtccg tttcggccct agcgtcctat tcgcgctcga tctttggggc cctgtacgcc
     8941 tacatgccct ccaccgcggg gacggtctcg gcggccgcac cggacgggac gtcctatagc
     9001 ggcaccatga cggacgtgtt ggtggcgtcg ccgacgtcgg gcctatggaa cttctttcgg
     9061 accggccaga gcagtgcggt gggcttgtac ctcgtgaccg tgccgggcca ttcctggtga
     9121 agaccatgcg caaaatcagc gatagttcgt gaactgcagg gggatgccga agtcggcttc
     9181 cttcaggtga gcgatggcct cttggagcac gtcgcgggtc ttgccggaga cacgcacctg
     9241 ctcgccttgg atcgcggcct gcaccttgag gccgctctgc ttgagcgact tcacgatggc
     9301 cttggccttg tcctcgccga tcccttggtt gatcttgatg tccatcttgt aggtgccgcc
     9361 gccggcttcc ttgatcggtt cgagttgcag cgccttcagc gagatcccgc gcttgacgca
     9421 cttctcctgg aatacgtcca gcgccgcttg aacgcgctgc tccgcgctcg aggtgatccg
     9481 gatctcgagg tcgccctgcc agttgatcga ggtcccggtt cctttgaagt cgtagcgctg
     9541 cgacagctcc ttgcccgctt gattcagggc gttgtcgacc tcctggcggt cgacctcgga
     9601 gacgacgtcg aatgaactgt ctttcgccat gtgatacaac ctccggccgt acgggtctcg
     9661 attcgatcaa gtctcggacc cgggtacact aatacgctcc aagggcgggt acccaagtgg
     9721 ccaaagggga cggactgtaa attcgttggc tcagccttca gaggttcgaa tcctctcccg
     9781 cccaccatct cgagcccgct ccggcgggct cgacgcgttc ccggcccgac gggccatccc
     9841 gtcggacgcc gcccgcccgc ccggccggtg aggcgctaca atgccccctc gcacgctggc
     9901 gtagctcaga cggcagagca cggccatggt aaggccgggg tcaggggttc gattcccctc
     9961 gccagctcca actccttgcg gcagaccttc gggctgcggc gtgaggcggg gtagctcagt
    10021 ggtagagcat gcggctcata atcgcagtgt cggcggttcg atcccgcccc ccgctaccaa
    10081 cccatagcgt ctcctgaacc ggaccggccc tcacggccgg tcttcttcat ctccgcttcc
    10141 attgaagttc cgaaatggaa cttcgcgggc tcttcggccg tcgtatagtc cgcttgcttc
    10201 ggtgtctccg gtttggccgg gaggaggcac cggcggcccc acccggcccg aggagacacg
    10261 catgctcaag cgcaccttgt tgatcgccgc gatcaccgcg atgctgctgt cgggcgccgc
    10321 ggttcgcgcc gacgcggctc cggccccatg gcccggcgtc aagcttttga cgaccgagga
    10381 cttcgtcgag gtcgtccgat ggcccgatga gccgttcatc ggcgacctcg gcatcgtcgt
    10441 ggcggccggg aaccgtccgt tcgagcttca cctgagcaag tccaactacc gcgcgccgat
    10501 ccgcgtcacc cagcacgacc gtaccgggcg acggagccgc ttcctggccg aggccaccaa
    10561 ggcgtccttc gcggaagggc tgaaggactt cttcatcgtc accgtgaccc gtcccgacgg
    10621 tacgcgcgtg cttcggcgtg atgccggctt ctgcaccgat gcctgggatc gaatgcgcct
    10681 gacgatctcg gctcccgaga cgagccgcta cccgaacgga tgcagcgaca ttccctacgc
    10741 caagggaacc gtgctcggga tcgaagccgg gtggggcatg ccgttgcgga ccatgccggc
    10801 cctgaacgtg ccggacgggg agtacaccgt cacgatctcg gtccgctacg gataccgaag
    10861 gctgttttcg atctccgagc aggacgcggt cgcgtcggtg gacgtgtcgc tggtgaccgt
    10921 ggacgaatgc tgcgtggagc ctccgccgga gatgcgtgcc cgccgccgcg ccgcggccgc
    10981 cgcgccgctg cgtccggtcc gcccgtcggg tgcgccggcg gcggcgccgg cgaccgacac
    11041 ccgtcccgat ctggtcgcca tccctgcgtg gaacatccgg atcggcaacc cggacgggca
    11101 cgtcgacgag ccccagccgg agccggaagg ccgcgacatt ctgcggttct cctcgaccgt
    11161 atggaacgcc gggcccgcgc cgctcgtcgt ggacggcttc cgcaacaagg gcgagcccga
    11221 gatgaccggc taccaatcct tttacaagaa cggcaagcgg gtgggctgga agcagatcgg
    11281 cgcgttcgag ttcgacgacc gtgacggcca ccagcactgg cacttcaagg atttcgcgca
    11341 gtaccggctg ctggacgccg gcaaaatgga cgtcttgccc gcgggcaagg aggccttctg
    11401 cctggccccg accaacgcga tcgacatgac cgtgcccggc gccgactgga gcgtccaccg
    11461 cgtcgagttc cccggcagca tgtgcggcca gcgcagctcg ctggcgatcc gtgaggtcct
    11521 gcaggccggc tgggccgaca cctacggaac cggcacgccg ggccaggagt tcgacgtcac
    11581 cgacctgccc aacggcacct acttcatcga ggtcgccgcg aacccgctgg gccgcctgat
    11641 cgagcgcgtc acctcgaaca acgtcgccct tcgcgagatc gtgctgggcg gcaccccggg
    11701 caaccggacc gtggtggtcc ggccctacca gggaatcgcg gcgtagcggc cggcggaatc
    11761 gcggccatag cggccgggcc ggaaccgtcc gtgggggccg ggtatcgccc ccacggacgg
    11821 accggtatac ttcccgaccg ctgtttcgaa tgcgaaaggg aaacgacggt ggccaagagc
    11881 gacaagcgtc cggggatcac gctcgcctgc gagcagtgca aacgacgcaa ctaccaaacc
    11941 acgaagaaca aggtgaacac gcgggaccgc ctgcagctca agaagtactg cgcctggtgc
    12001 ggctcgcaca ccgaccaccg cgaaacccgc tagccagcgg ccggccatcc ctattctgtg
    12061 gaccagcgac tacccatacg ggtgttcaat gtccacagaa cgcatcgccc gaccaaggcg
    12121 attgccggtt tgcacgggga atggccccgc gaagatactg ggcccctgta aacctaccga
    12181 cgagtaacaa aggggagggg accgactgtg ggccgccttg acgatcgcgt cgccatcatc
    12241 accggaggag gccgcggcat cggccgcgcg accgcgctcc gcttcgccgc cgaaggcgcg
    12301 gccgtcgtcg tcaacgacgt cgatgccgac ccggcggaag agaccgccga gatcattcgc
    12361 aaggagggcg gcaaggcgtt ggtgtcgctg cacaacaccg tcgaggctgc caacgcagaa
    12421 gcgatggtca aggccgcgct cgatgagttc ggcaagctcg acattttggt caacaacgcc
    12481 gggatcacgc gcgacaagac cttccacaac atgacgcccg acatctggga tctcgggatc
    12541 aaggtcaatc tctacaccgc gtatcactcg accttctacg ccgtgcagca catgcgctcg
    12601 gccgccaaga aggagctcga ggagaagggc gccgtttcct atcaccgcaa gatcaccttc
    12661 accgcgtcgt cggtcgcgct gaccggcaac ccgggccagg cgaactacac ggccgcaaag
    12721 ggcgcgttga tctcgctgac caagacgctc gcgcgcgagc tgggtccgtt caagatcaac
    12781 tgcaacgccg tcgcgcccgg cttcgtcgaa acgcgcctga ccgccgcgaa ggaagaggga
    12841 agcgacctgg gcatcccgga ccagcttcgc aacatggcgc tgatgatgat cgcgctcggc
    12901 cgtgcggggc tgcccgagga tctcgccaac gtccatctgt tcctgtgctc gcaggacagc
    12961 gacttcgtgt ccggcgtgac cattccggtc accggcgcgc agctcggcgc ctaaaggaga
    13021 gtgcatcgat gccactgaac acagcgctgg tcggcaagga gtatccgaag tcgcccgtct
    13081 acgaggtggg ccgcgagaag attcgcgagt tcgcgatggc catcggcgat ccgaacccgc
    13141 tctacaccga cgagtcggcg gcgaaggcgg cgggctacgc cgacatcatc gcgccgccga
    13201 cattcctgac gatcatcggc ttcaagttcg gcccctcgat cgtgctcgat cccgagctcg
    13261 gcctggatta cacgcgcgta gtgcacggcg agcaggagtt cgagctgaag cggccggtcg
    13321 tgccgggcga ccgtctgacc ggcacgccga agatcgtaga catctcggca aagggcaaga
    13381 acgagttcct gacctacgag gccgtcatca ccgccgagag cggcgaagag gtcgcgatcg
    13441 ctcgttcgac catcgtctcg cgcggaaccg cgccgcagga gggctgagcg acatgacggc
    13501 caaggtcaat tatgcagacg tctccgaagg cgaagagctc aagccgtgga cgtacaacgt
    13561 ccagcggatg aacctcgtga tgtacgcggg cgcgtcgggg gacttcaaca tcatccattg
    13621 gaacgaagag atcgcgaaga tggtcgggct gcccaacgtg atcagccacg gcatgttcac
    13681 gatggccaag gtcggccagt acgtcaccga ttgggcgggc gacgcgggcg cggtcgtgcg
    13741 cttcaagacg cgcttcacca agccggtcgt ggtggacaac caggccggca acaacgtcac
    13801 ggtgaccggc acggtcaagc agaagctcga cggcaaccgc gtgctgatcg agctgagcgc
    13861 gaccacgcag gaaggcgaca actgcgccgc cgcggaggcg gaggtcgtgc tggcctagcg
    13921 tgaacgcgat tcccgagcag gtcgccgcgc tgctgcgcga acgcgatgag gcgcgagccg
    13981 cgaaggatta tgcgcgctcg gatgcgctgc gcgaccgcat cgacgcgctg gggttcaccg
    14041 tccgcgatac gccgcagggg ggcgtcgccg aggccaagcc gaagttcgag gtgctcgatg
    14101 cggccgatgt acccaacatg ctcgaccggc cggccgcgtg cgcgatcagc atccacgttc
    14161 tgtacgaggg atttcggaac gacctttctc gattcgttga ttcggtcgcg cgtcacggcg
    14221 cgggccacga ctacgagatc gtgatcaccg aatgcgcttc gcccgagggc gcctcgtccg
    14281 acggggaatg gatcgagtcg ctggccggcg agcgggtccg ggtcctccac ttcgcggacg
    14341 atccgggatg ggcggcgggg cgcaacgccg cgatcggcgt cagccgcggc gcgctgatcg
    14401 cggtcgcgga cctgtcggtc gagctgaccg gcgacctttt ggggccactc gtcaagattt
    14461 ttgacgatgg gcgggtgggc gtggccggcc cgtgggggtt gcggacgcgg aacctgcgcg
    14521 acttcgaaga ggtcgaagga cccgaagtcg cgcacgcgat ccaggggtac tttctgacga
    14581 cccggcgcga catcgcccgc gagcatcgct tcgaccgcaa gttcgcttgg taccggcacg
    14641 ccgatatcga cttgagcttc gccatccgcg cggccggtta ccgggccgtc gcgaccgagc
    14701 ccgccgccac ccgccatacc caccgggctt ggatggccct ggacgaagac gagcgcgcga
    14761 aacggtcgag acgcaacttc tcccggtttc tcgaccgatt caaggatcgc accgatctga
    14821 tcgagacggg ggtgtaacct cggactggga aagcgcgtcc gacctggcgg aacgtggtgg
    14881 agatccctcc ggacctcgtg gaagcctgcc gtcgcggtga cgtcaaggcc ttcgaagcgt
    14941 tggtccgggc gaccgaacgg caggtgtaca cggtcgtcta ccgcatcgtc ggcaatcacg
    15001 acgacgccgc cgacgtcgcg caagaggtct acctcaaggt ctggaaggcc atccgccagt
    15061 tccggggcga cgcggccgtc acctcgtggc tctatcgagt cgcgaccaac gcggcgatcg
    15121 catcgctgcg caagcgcgga cggctagccg acccggtcga gccggaccgg ctcgccgaga
    15181 tcgaggcgcc ggcggtcacg ccggccgtcg ataccggcga cgtcgagcag gccctgaccc
    15241 ggctgccggc cgcgtaccga accgtcctgg tgatgagaga gacgtacggc atgagcatcg
    15301 aggagatcgc caagcagatg ggaagttcga ccggcgcagc caaggtccgg ctgcaccgcg
    15361 cccgtctgcg tctggccgac gagctggaac gtgccggcgt ggtcgtaccg ctgcagcgaa
    15421 gggggaagaa gacgtcgtga actgcgaacg ggtccggccg tacctgacgg cccatgccgc
    15481 cggcgaactg ccggcccata ccgcgtcctg ggtcaacccg cacgtcgagg actgcccggt
    15541 ctgctcgtcg ctgtcgctgc gtcacgccac gatccgcgtc gcccttcgca cgctgccgga
    15601 ggccaccgtc gatccgccgc ccggcttcgc cgcgaccgtc gcgcgccgcg cggcccgcct
    15661 gaagcgcgcc cggccgatcc cgatcccgcc gatgatcccg cccgatgcga tccgggttct
    15721 gaccgacaat cgggacgcca tcctcggctc cgccggggtc gcgctggggg cggccggggt
    15781 cgcttggatc gcctggcgct cgatgcgctc ccgcaagccg gggccgcgga ccgcgtaaac
    15841 cgcctgctcg ccaggggggg tgtcgcttta cacccttcaa gcgcggcccg tatactggcc
    15901 gcttgttggc gacaaggggg taccccagcc gacggagggc cgtagctcaa tttggcagag
    15961 cgctggtctc caaaaccaga ggttgggggt tcgattccct ccggccctgc cagcacgaat
    16021 aacccgcagg aagcgttgcc gcggacccgg gtccgcggcg tcgtcgtgaa cgggccgcag
    16081 aacgcggccg aagggataag accgtggtca accgagcaac gaagcgactg ctcgccaagc
    16141 agcagaagaa agagcagggt ggcaagccgc gtacgcgcga agaggcggtc gccgagcgcc
    16201 gcagcgcgat cgagcgcaag aagcgcacgc ctcctcgtca gttcctgcgc gaggtgcgcg
    16261 gcgagctgaa gaaggtcgcg tggccgtcgc gccgcgaagt ctcgacctat acggtcgtgg
    16321 tgctcgtttc ggtgatcttc atcacgggca tcgtcttcgc gctcgactac ggcttcaacc
    16381 aggcaatctt ccagacgttc tccaaatagg tctttcgacc gggaacccaa attcgatgga
    16441 cgactcctca gtcaacggcg tgcgcgccga ccgcgaaaac gacgacgagc cccagccgcc
    16501 ttccggcaac ggacacggca acgggtccgg cgacgacacc gcgcaagacg cccccgaggc
    16561 gcaggccgga acagccgcga tcgacatcgg cgatgtgccg gtggatgccg ccgacgtcgc
    16621 ggacgccatg gcgacgtccg gcgagcacct cacgtccgag gcggaccctg ccacgatcga
    16681 ggagcagtcc ggcgcgttcg agcaggccga ggagccgcag gctgaggagc ccgccgtgga
    16741 gtcgaacggc cacgcggcgc cggccgagcc ggccgcggcc gccgagcccg agccgaaagc
    16801 cgagcccgag ccgaaagccg agcccgagcc ggcgaagccg aagggcgcca agggcaagga
    16861 cgacggcggg a
//
LOCUS       DATLFO010000230         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87288, whole genome shotgun sequence.
ACCESSION   DATLFO010000230 DATLFO010000000
VERSION     DATLFO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87288"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1030
                     /locus_tag="VM841_09280"
     CDS             14..1030
                     /locus_tag="VM841_09280"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HVL90409.1"
                     /translation="MNADLRSTAKALVAPGRGILAADESSGTIKKRFDSINVESTEEN
                     RRAYRQMLFTAKGANEFISGVILFDETLRQSADDGKTFVETLNAQGIIPGIKVDKGAH
                     PLPFSPDEKVTEGLDGLRQRCEEYVQLGAKFAKWRAVIDIKGDEIPSQYCIDVNAHAL
                     ARYAAICQETGLVPIVEPEVLMDGDHTIERCDEVTRATLKTTFAALFDQRVDFESMLL
                     KPNMVVAGKKCPAQPDNATVAEMTIACLRATVPAAVPGIVFLSGGMSDEDATDRLNEM
                     NKRGPNPWELSFSYGRALQAPALKAWKGEAANLEAGQKAFSHRAHMNGLARSGEYSAD
                     KESA"
     gene            1175..1378
                     /locus_tag="VM841_09285"
     CDS             1175..1378
                     /locus_tag="VM841_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVL90410.1"
                     /translation="MAVIKSIDLVGVSTESWRDAAQAALAEASKTLRGIEGMQVLETS
                     AVVDGDKIKEYHTHVRISFRLDR"
     gene            1654..2946
                     /locus_tag="VM841_09290"
     CDS             1654..2946
                     /locus_tag="VM841_09290"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVL90411.1"
                     /translation="MPATVLVGAQWGDEGKGKITDILGPETDVIVRYQGGNNAGHTVI
                     AGDVTLKLHLVPSGILSPGVVPMIGDGVVVDPAVLIEEMDALAAQGISTEHLLVSVNA
                     HLIMPYHRLLDAVIERHLGRSQLGTTKRGIGPAYADKAARIGLRVQDLLDMKIFEQKL
                     EVVLKERNAVIAKIYNRLPLKTAEIMEQYGEFAKRLAPHIADTSLEIWSALRAGKRVL
                     FEGAQGTLLDVDHGTYPFVTSSNPVAGGACTGAGVGPREISRVIGIAKAYISRVGAGP
                     FPTEDLGPDGDRMIDIGQEYGTTTGRRRRCGWMDAVLLRYAARLNSLSDVFMTKLDVL
                     SGFDEVKICTAYRHDGTTYDEFPPHQSIFHNAEPVYETMPGWDGDISHARTFADLPAE
                     ARDYVQRIEQVAGVPITLVSVGPERDQTVRLDPVGVPA"
     gene            2946..>3871
                     /gene="purD"
                     /locus_tag="VM841_09295"
     CDS             2946..>3871
                     /gene="purD"
                     /locus_tag="VM841_09295"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HVL90412.1"
                     /translation="MRVLVVGSGGREHALALGLARSASVEKVFCAPGNAGIADVAACE
                     ALDPNDPSAIGDFAEAQGTDLIVIGPEAPLVDGLADILRARDLRVFGPGAEAAQIEGS
                     KAYAKDVMRRAGIPTAFAETFTDSERAINFLREFGAPCVVKADGLAAGKGVRVCEDLI
                     AAADAVDDMLRQRAFGEAGATIVIEECLTGEEISVFALSDGRRVVPLASAQDHKRIGE
                     GDTGPNTGGMGAYSPVPHLDVVDRVMAETFEPLVARMAADGTPFTGVIFGGFMVSEAG
                     PKVLEFNVRFGDPEAQTIVPRLESDLGELLFA"
BASE COUNT          651 a         1312 c         1320 g          588 t
ORIGIN      
        1 gcaaggagga accgtgaacg cagaccttag aagcaccgcg aaggcgctcg tcgcgcccgg
       61 ccgaggcatc ctcgccgccg acgagagcag cggcacgatc aagaagcgct tcgactcgat
      121 caacgtcgaa agcaccgagg agaaccgccg cgcctaccgc cagatgctgt tcaccgccaa
      181 gggcgccaac gagttcatca gcggggtgat cctgttcgac gagacgctgc gtcagtcggc
      241 ggacgacgga aagaccttcg tcgagacact gaacgcgcaa gggatcatcc ccggcatcaa
      301 ggtcgacaag ggtgcgcatc ccctgccgtt ctcgcccgac gagaaggtca ccgaaggcct
      361 cgacggtctg cggcagcgct gcgaggagta cgtgcagctc ggagcaaagt tcgccaagtg
      421 gcgcgccgtg atcgacatca agggtgacga gattccgtcg cagtactgca tcgacgtcaa
      481 cgctcacgcg cttgcccgtt acgccgcgat ctgccaggag accggcctgg tgccgattgt
      541 cgagcctgag gtcctcatgg acggggacca cacgatcgag cgctgcgacg aggtcacgcg
      601 agccaccctc aagacgacgt tcgcggcgct gttcgatcag cgtgtcgact tcgaaagcat
      661 gctgctgaag ccgaacatgg tcgtcgccgg aaagaagtgt cccgctcagc cggacaacgc
      721 caccgtcgcc gagatgacga tcgcctgcct gcgcgcgacc gttccggccg cggtgcccgg
      781 catcgtgttc ctgtcgggtg gcatgagcga cgaggacgcg accgaccgcc tcaacgagat
      841 gaacaagcgc ggtccgaatc cgtgggagct ttcgttctcg tacggccgcg cgctccaggc
      901 ccctgcgctc aaggcgtgga agggcgaggc cgcgaacctg gaggccggcc aaaaggcctt
      961 ctcccaccgc gcgcacatga acgggctcgc gcggtccggc gaatactccg ccgacaagga
     1021 aagcgcgtag caccgcgatc gaacgggccg gcgatccgcc ggcccgttcg ctatctgcgg
     1081 cccgcgcccg ggcggcgtca aggttgcatg gccggtttga cgctgcggcg gcccgctccc
     1141 ctagaatcgc cgcgacatcg cgacgggaga cgttatggcg gtaatcaagt cgatcgatct
     1201 agtgggtgtc tcgaccgagt cgtggcgcga cgccgcgcag gcggcgctgg ccgaggcgag
     1261 caagaccctg cgtggcatcg aggggatgca ggtcctggag acctctgccg tcgtggacgg
     1321 tgacaagatc aaggagtacc acacgcacgt ccggatcagc ttccggctgg atcgataggc
     1381 cggggtgacc ggccgattcg atcggcggga gcccgggcgg aaagcccctg aaaaggccac
     1441 ggagcggggc gagcgccccg cttcttccgt gcccggccgc ccgaggggtt cgccccgctc
     1501 cgttgctata atgacgccgg tcccgcgacg cagcgcgacc tcctgggaac ctctttgaga
     1561 tctcgcttgc catggccccg ctgcctgggc cgatacgcct gggccttcgc cccgtacccg
     1621 cgcccacgtg ccttcgatcg gagagagccc tagatgcctg cgaccgtgct cgtcggtgcc
     1681 caatggggcg acgaaggcaa aggaaagatc accgacatcc tcggtcccga gaccgatgtg
     1741 atcgtgcggt atcagggcgg caacaacgcc ggccacaccg tgatcgccgg ggacgtcacc
     1801 ctcaagctcc acctcgtccc gagcggcatc ctgtcgcccg gcgtcgtgcc gatgatcggc
     1861 gacggcgtcg tggtcgatcc cgccgtgctg atcgaggaga tggacgcgct cgccgcgcag
     1921 ggcatctcga ccgagcattt gctcgtgtcg gtcaacgcgc atctgatcat gccgtaccac
     1981 cgcctgctgg acgcggtcat cgagcggcac ctcggccgct cgcagctcgg gacgacgaag
     2041 cgcggaatcg gtccggccta cgccgacaag gcggcccgca tcggactgcg tgtccaagat
     2101 ctgctggata tgaagatctt cgagcagaag ctcgaggtcg tgctgaaaga gcgcaacgcc
     2161 gtcatcgcca agatctacaa ccggcttccg ctcaagacgg ccgagatcat ggagcagtac
     2221 ggcgagttcg ccaagcgcct cgcgccgcac atcgccgaca cctcgctcga aatctggagc
     2281 gcgctgcgtg cgggcaagcg tgtgctgttc gaaggcgcgc agggcacgct gctggacgtg
     2341 gaccacggca cctatccgtt cgtgacctcg tcgaatccgg tcgcgggcgg cgcgtgcacc
     2401 ggggccggcg tcgggccgcg cgagatctcg cgcgtgatcg gcatcgccaa ggcctacatc
     2461 agccgcgtcg gcgccgggcc gttcccgacc gaggacctcg gtcccgacgg cgaccgcatg
     2521 atcgacatcg gccaggagta cggcaccacc accgggcggc gccgccgctg cggttggatg
     2581 gacgcggtgc tgctccgcta cgcggcgcgg ctgaactcgc tcagcgacgt cttcatgacc
     2641 aagctcgacg tgctgtcggg tttcgacgag gtcaagatct gcaccgctta ccggcacgac
     2701 ggaacgacct acgacgagtt tccgccgcac cagtcgatct tccacaacgc cgagccggtc
     2761 tacgagacca tgcccggctg ggacggcgac atctcccacg cccgcacctt cgcggatctg
     2821 ccggctgagg cacgtgatta cgtgcagcgc atcgagcagg tcgcgggcgt gccgatcacg
     2881 ctggtgtcgg tcggtcccga gcgcgaccag accgtacgcc tcgacccggt aggagtcccc
     2941 gcctgatgcg cgttctggtg gtcggcagcg gaggccgcga gcatgcgctc gccctggggc
     3001 tggcgcgcag cgcctcggtc gagaaggtct tctgcgcccc cggcaacgcg ggtatcgccg
     3061 atgtcgccgc ctgcgaggcg ctcgacccga acgatccctc ggcgattggc gacttcgccg
     3121 aggcgcaggg caccgatttg atcgtgatcg gacctgaggc gccgctcgtg gacggcctgg
     3181 ccgacatcct tcgcgcccgc gaccttcggg tcttcggacc cggcgccgag gccgcgcaga
     3241 tcgagggctc caaggcgtat gcgaaggacg tcatgcgccg cgccgggatc ccgaccgcgt
     3301 tcgccgagac cttcaccgac agcgagcggg ccatcaactt cctgcgcgag ttcggtgcgc
     3361 cgtgcgtcgt caaggccgac gggctcgcgg ccggcaaggg cgtgcgcgtg tgcgaggatc
     3421 tgatcgccgc cgccgacgcg gtcgacgaca tgctgcggca gcgcgcgttc ggcgaggcgg
     3481 gcgcgacgat cgtgatcgag gaatgtctga ccggcgagga gatctcggtg ttcgccctct
     3541 cggacggccg gcgcgtcgtg ccgctcgcgt ccgcgcagga ccacaagcgc atcggcgaag
     3601 gcgacaccgg tcccaacacc ggtggcatgg gcgcgtactc gccggttccg cacctggacg
     3661 tggtggaccg ggtcatggcc gagacgttcg agccgctggt cgcgcggatg gccgccgacg
     3721 gcacgccgtt caccggcgtg atattcggtg gcttcatggt gagtgaggcc ggcccgaagg
     3781 tgctcgagtt caacgtgcgc ttcggcgatc ccgaggcgca gacgatcgtg ccgcggctcg
     3841 agtcggatct tggcgagctg ctcttcgcct g
//
LOCUS       DATLFO010000231        13779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_123403, whole genome shotgun sequence.
ACCESSION   DATLFO010000231 DATLFO010000000
VERSION     DATLFO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123403"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VM841_09300"
     CDS             complement(<1..270)
                     /locus_tag="VM841_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL90413.1"
                     /translation="MTDHAQESRTRRLSERVTMVEAPNPSPMTLEGTNTYVVGGSNHV
                     IVVDPGPNVPEHLDNIVAAAGDAEIVGVFLTHWHADHSAGVRAFAD"
     gene            complement(279..1109)
                     /locus_tag="VM841_09305"
     CDS             complement(279..1109)
                     /locus_tag="VM841_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL90414.1"
                     /translation="MTEPDAKRHTFLLQDAPEQSVEPRDSATIILARDGAAGLEVFML
                     ERVLKADFAGGAYVFPGGTFDEGDLDPTLAGLTDGVTPDQAAEAIDAPLDRALAFYLC
                     AIRETFEESGALMARRDGTLIGHDPALQGARPLLNDGTLKLKEFAETHALRFAADLLH
                     PWSRWVTPEASPKRYDTRFFVAAFPDGGEALAHDEIESQSSTWIRPADALARHAEGRF
                     SVIFPTRVTLTQLSRFDRVADLIAASPGRDLAPVQPEIVRVGEAIKIMVPGDPEPYDP
                     "
     gene            complement(1164..2666)
                     /locus_tag="VM841_09310"
     CDS             complement(1164..2666)
                     /locus_tag="VM841_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90415.1"
                     /translation="MSIEDRLRDQLAAAAQRAPYPGELPAAVQRRARRRRAGSTVLAT
                     VVAALVAAGSVVVAGSIGGVEPVDASQCPPVDPPLRGSVRIERGRNATSDIAAGTGSV
                     WAIQGGALTRYRPGGKPAAVALPHPGTEGTASVSVVAASCREIWAFGSVLKVVPFESH
                     YPWLLPTNGPVPYGGMRPVIESTGTFAWRIDPDGKADEPIRLASGDAPADAVVTEHGV
                     LYIKGSDVLRIASGSRVVETVLSVPDRSLAAIAADAREVWLLGAARERPCARGASCPV
                     PPGEAIKVDATTMRTLGRYPLTIAAAPSGGSIALHETGAWFASATPGRIVRIDRVTGA
                     ESAVTFGARFAAHAIAATDSYIWVVTFDKDAGGADGYLVRVLASAAGVPDFRRLGFAF
                     PLTMTVAGETAWILAEELYWEPLMSEIPAVGRPVEGTAARVGGTRFSPLHEGQILARV
                     PRDAQGRCDRDAAIDESTHPYRGVRFGFTADCLVYVKQIWDSEPVTVLDG"
     gene            complement(2663..3160)
                     /locus_tag="VM841_09315"
     CDS             complement(2663..3160)
                     /locus_tag="VM841_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HVL90416.1"
                     /translation="MAEAESHEKARSRLAALYERHAPGALRFAYLLTGDTTEAEDLVH
                     DAIVRMAARFTDLRSEDAFPAYLKRAIVNQAASIGRRHRIEREWRERNTRPPVVMPAE
                     PGPGDELWRAMTRLPARQRAALVLRFYEDLSERQTADILGCRPGTVKSLVSRGLASLR
                     GVIEP"
     gene            3276..4448
                     /locus_tag="VM841_09320"
     CDS             3276..4448
                     /locus_tag="VM841_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90417.1"
                     /translation="MDFSVAPELEEVRSSYRSFLEREVRPVENRFAQQLQGDEWTPEM
                     REAGMELRRKSAEIGYYGAHLPEDVGGWGLSTLGMTLLVEETGRSGLRFATYVLGPPN
                     PEAPTPLLLEGTPEQQKKYLVPLIKAEKTMCFALTEPDAGSDAQSIRTRAVKDGDHYV
                     INGVKHFITNGERADFAVVFAVTDPDKRAQGGITAFIVEKGTPGYTVGKKQRTMAGDS
                     NQSELIFEDCRVPAANILGPEGYGFFSAMKFLNAGRAYIGAMCLGISDYLIRICTEYA
                     NERKAFGKPIGKFQAIQWMLADSAVEVEAARWLTYRLAWMCDQGEQPMRESSMVKLYN
                     TEMVGRVADRAVQIYGGMGYLTEGPVERIFRWVRMLRIVEGTSEIQRLVIARTLGL"
     gene            4445..5200
                     /locus_tag="VM841_09325"
     CDS             4445..5200
                     /locus_tag="VM841_09325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90418.1"
                     /translation="MSDHIARNRATWAGFAPDYVHNGRVSWARDEPKWGVFGIDDDRI
                     LPAGIEGKDVVELGCGTAYVSSWFARRGARSVIGLDLTGEQLDTANALRAEHGLPVAL
                     VQGNAECTPFPDASFDIAFSEYGASLWCDPAAWIPEAARILRPGGELTFLTNGIFIEV
                     CIPDTDGEGPATDRMLRPYFGLGRTEWPEEPGVEFHLTHGDWIRLLRASGFEILDLIE
                     IRVPADATTSSPWVSTDWATKWPVEEVWKARKR"
     gene            5211..6539
                     /locus_tag="VM841_09330"
     CDS             5211..6539
                     /locus_tag="VM841_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL90419.1"
                     /translation="MLFDINYALVGETAVAYTVIGDGPVDLLIAPPVNSHLDIQSELP
                     EWVEFHQRLGQFARVFVFDKRGTGLSDPLPGTGVSTLEERVDDMLAIMEHAGSERVAV
                     MGYGEGGAAAALFAATHPDRTSHLILLNTTACLRRRDDYPWGWDPVDTPMANWLANFG
                     KEEPLLKARLGSALSDPRIRMWATRWVRSSSSPLLYRALQKVAMDIDVRKVLPAISAP
                     TLVMHCTDNHILDPQNGRFLAAHIAGARFVELPGDEHYPFLGDNAALIAEIEEHLTGS
                     RTVEEAERVLATVLFTDIVDSTQTAARMGDREWKTLLTRHHEAVRDEIARHRGREIAT
                     AGDGFLATFDGPARAVRCAWNVHKAVEPYGIRLRAGLHTGEIELHGDTIAGIGVHIAA
                     RVASIAGAGEVLVSRTVRDLVAGSGISFVDRGRHTLKGVPDEWEIYAAQH"
     gene            complement(6536..7651)
                     /locus_tag="VM841_09335"
     CDS             complement(6536..7651)
                     /locus_tag="VM841_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90420.1"
                     /translation="MDTLLRMTVEQASAVACRGLAKRFGNVVALDEVTLTIAAGEIVA
                     LLGPSGCGKTTFLRVIAGFERADAGVVELEGRVVDGPDAFVAPERRRAPLVFQDYALF
                     PHMTVAANVGYGVARDERGRVEEVMRLVGLEGLGDRYPHELSGGQQQRVALARALAPR
                     PAIVLLDEPFSNLDADLRARVRGEVRTIIRAAGATAVFVTHDQSEAFDIADRIAVMHA
                     GRIEQLGRPEEIYHVPETRFVADFVGRAEFIPATVTADRIDSELGSFAFAPGEVRPGE
                     AELMLRPEDIVLANGNGGATAEVIERRFQGPVNVSVVRLPSGRVVSSVHSSQNLFRPG
                     DRVHVSFAPDHLVVFRGDRRIAWMAVREHTGTDVPDA"
     gene            complement(7651..9288)
                     /locus_tag="VM841_09340"
     CDS             complement(7651..9288)
                     /locus_tag="VM841_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVL90421.1"
                     /translation="MTRAAPAIRRRPERRASRRRIPRWLGAAGFAVAAGVALPAVYLV
                     VRSWSLGPDGIAGTVTDARTVGLLVRTTLLAIAVTGTSVAIAVPLAWLTTRTDLPGRR
                     IWTVLTALPLVIPSYVGGYVFIAALGPRGLVQGWLEPLGVQRLPSIYGFGGAWLVLTL
                     FTYPYVLLIVRAAIRGLDPALEEASYGLGRGRRFTLRRVVLPQLRPAIVAGSLLVALY
                     TLHDFGAVSLLRFDSFTRAIYVSYQASFDRSRAAVLGLMLVALTLIVLVLEARARGRA
                     AFYRRHPGGRRPAPVMPLGRWRWPAVAACALVAGFALLLPVAVIVLWLVRAAAEGEPV
                     RVAVTAAWNSLRVAGLGAVVALIAAWPVAVLGVRFPGRGARALERTTYVGYALPGIVV
                     ALALVFFGARYVPVLYQTIAMLAFAYAVLFLPQAGGALRTGLLQIDPHVEEAAQSLGS
                     SRRRTALRIVMPLVRPGVLAGLALVFLTAMKELPATLLLAPTGFSSLATEVWGATNAA
                     SFGRAALPALLLIVLASGPLAALLLSGPGERRLRERP"
     gene            complement(9309..10334)
                     /locus_tag="VM841_09345"
     CDS             complement(9309..10334)
                     /locus_tag="VM841_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HVL90422.1"
                     /translation="MRYGSKTALAAVLLALVAAACGASEPGGTGGDGGQTTLTVYSGR
                     EKDLVQPLFEKFEAANPSIKLAVRYGDSAELAAQMLEEGGNSPADVFFAQDAGALGAV
                     ANGLANLDQEILSSVPEAFRSDAGKWVGISGRARVVVHNTDSVKPADLPASIEGFTDP
                     KWKGKIGWAPTNGSFQAFVTGLRVLKGDEAARTWLRGIKANDPVVYPKNTPIVEAAAA
                     GEIQVGFVNHYYLLRLKKEQPAMKAANHFFKGGDPGALVNVAGAGILSTSKSKAAAST
                     FVKYLLSAEAQQFFATETFEYPLATGVEASADLPKLSALEPPDLDLSKLSDLEATLTM
                     LRDEGIL"
     gene            10470..11813
                     /gene="selA"
                     /locus_tag="VM841_09350"
     CDS             10470..11813
                     /gene="selA"
                     /locus_tag="VM841_09350"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HVL90423.1"
                     /translation="MAETDLLRKIPQVDAVLRDDAVRGVAALLGEAAAARLVRDAIAA
                     ARAAAQKGTEPPSAAAMATEIAARATALHGSRLRRAVNATGVILHTNLGRAPLSREAI
                     AAVADAAGPAPLEFDIAAGERGVRAPLAAFLAATLTGAEAAHVVNNNAAALLLMLAAI
                     ARDREVIVARGELIEIGGEFRLPSIMEASGAMLREVGTTNRTHPRDYRGAIGERTAAI
                     LLVHPSNYRVVGFTTQPSLPDIGAIAREAGVPLLYDIGSGLLAPDEHLPDEPDARGAL
                     DGGADLICFSGDKLLGGPQAGVLAGRKDLVAACKRHPIARAVRADKMTLAAMEATLLQ
                     IARGAPTPVASMLSADTETLRSRCESLASGLEGASVIEGQSVAGGGSVPGHGIASPVV
                     AIACDKPERVTAALRANDPPVIARIDDGRLIVDPRTVDPSDDAALRAALERAIRS"
     gene            complement(11800..12501)
                     /gene="phoU"
                     /locus_tag="VM841_09355"
     CDS             complement(11800..12501)
                     /gene="phoU"
                     /locus_tag="VM841_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HVL90424.1"
                     /translation="MTEPRRHFHDELRSLETNLVLEGERALALLNKSVRAILARDGAL
                     ADQVIDEDDEVDRLYMSIEKGTLELLALQTPVASDLRLLSAMIHINLHLERIGDMAVN
                     IAKAVKLTDELPTRASILDSIGEMATIAERMVSAALTAFQNRDQALAMQLPQMDDPID
                     RINRGMVAEVAACASDPGLLEWGLRIMIVSRQLERVGDHAVDIGEQVAFLLTGEFREF
                     TDASHPELERKVKSE"
     gene            complement(12523..13230)
                     /locus_tag="VM841_09360"
     CDS             complement(12523..13230)
                     /locus_tag="VM841_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90425.1"
                     /translation="MKILLVEDEETLREAVRYGLEREGFRVSLARDGKEALEKFRGGG
                     ADVILLDLMLPEVSGLDVCKAIRARSPVPIIILTAKDSEADKVLGLELGADDYVTKPF
                     SMRELIARVRAVSRRGSVPVSPGAVALKIGGVTLDPDAHEVRVDGAGLELPPKEFELL
                     HSLMSRAGRLVTRQTLIDEVWGPEYVGDTRTLDVHVKRLRQKIESDPRSPRYIKTVRG
                     LGYKFEDPKTGSGRDEA"
     gene            complement(13227..>13779)
                     /locus_tag="VM841_09365"
     CDS             complement(13227..>13779)
                     /locus_tag="VM841_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90426.1"
                     /translation="RIQTETDRLARLVADLLDLSRIESEMPLAIADTPASVIVEDAIE
                     RVSAFAEAKGTAISLEQPDRALVRADAGAAATALANLLDNAVRYAPAGTPVLLAVAST
                     GRVVSFTVADEGPGIPSEELGRIFERFYRVDKARARATGGTGLGLAIVKHVAERHGGR
                     VEASSEFGRGSRFVLVLPAGGAA"
BASE COUNT         2212 a         4880 c         4650 g         2037 t
ORIGIN      
        1 gtcggcgaac gcgcggacgc ctgcgctgtg gtctgcatgc cagtgcgtca ggaagacgcc
       61 gacgatctcg gcgtcaccgg cggcggcgac gatgttgtcg agatgctcgg gcacgttcgg
      121 gccgggatcg acgacgatga cgtgattcga tcccccgacg acataggtgt tggtcccttc
      181 cagcgtcatc ggcgacgggt tgggcgcttc gaccatcgtg acccgctccg acagccggcg
      241 ggtgcgggac tcctgcgcgt gatcggtcat gacggggcct atggatcgta gggctcggga
      301 tctccgggca ccatgatctt gatcgcctcg ccgacgcgaa cgatctcggg ctggaccgga
      361 gcgagatcgc ggccgggcga ggccgcgatc aggtccgcga cccgatcgaa tcgcgacagc
      421 tgcgtcagcg taacccgcgt cgggaagatc acgctgaagc ggccctcggc gtgccgggcc
      481 agtgcatccg ccggccggat ccacgtgctg gactgcgatt cgatttcgtc gtgggccaga
      541 gcttcgcctc cgtcggggaa ggccgcgacg aagaagcgtg tgtcgtagcg cttcggcgag
      601 gcttccggcg ttacccagcg cgaccacgga tgcagcaggt cggccgcgaa gcgaagcgcg
      661 tgcgtctcgg cgaactcttt caatttcaac gtgccgtcgt tgagcagcgg ccgcgcgccc
      721 tgcagcgccg ggtcgtgccc gatcagcgtg ccgtcgcggc gcgccatcaa cgcaccggac
      781 tcttcgaagg tctcgcggat cgcgcacagg tagaaggcaa gcgcccggtc gagcggcgca
      841 tcgatcgcct cggccgcctg atcgggcgtt acgccgtcgg tcagtccggc cagcgtcggg
      901 tccaaatcgc cttcgtcgaa ggtgccaccc gggaacacgt acgcgccgcc cgcgaagtcg
      961 gccttcagga cccgctccag catgaacact tccaagccgg cagccccgtc gcgcgccagg
     1021 atgatcgttg ccgagtcacg cggctcgacg gactgctcgg gcgcgtcctg cagcaagaac
     1081 gtgtggcgct tcgcatcggg ctcggtcatc gcccgtcatc gtattcgccg cgctccgcgg
     1141 gcgcgattcg aaaagcacgg cgatcagccg tccaggaccg ttaccggctc cgagtcccag
     1201 atctgcttga cgtagaccag gcagtctgcc gtaaacccga agcggacccc tcggtacggg
     1261 tgcgtgctct cgtcgatggc cgcgtcgcga tcgcagcgac cctgcgcatc acgtgggacc
     1321 cgcgcaagga tctgcccttc gtgaagcggg gagaatcgcg tgccgccgac gcgcgccgcg
     1381 gttccttcga cgggccggcc gaccgcaggg atctcgctca tcagcggctc ccagtacagc
     1441 tcctcggcca gaatccacgc ggtctcgccg gccaccgtca tcgtgagcgg gaacgcaaag
     1501 cccaggcggc ggaaatccgg cacccccgcc gcgctcgcaa ggacgcgaac gaggtagccg
     1561 tcggctccgc cggcatcctt atcaaatgtg acaacccaga tgtagctgtc ggttgctgcg
     1621 atcgcgtgcg cggcgaagcg ggcaccgaag gtgacggccg actcggcacc ggtcacgcgg
     1681 tcgatccgaa cgatacggcc cggtgtcgcc gacgcgaacc acgcgccggt ctcgtgcaag
     1741 gcgatcgatc cgccggacgg ggccgccgcg atcgtcagcg ggtagcggcc gagggtgcgc
     1801 atcgtcgtcg catcaacctt gatggcctcg cccgggggca ccggacagga tgctccccgc
     1861 gcgcacggcc gctcgcgcgc cgctcccagc agccacacct cgcgcgcatc ggcggcgatg
     1921 gcagcgagac tgcgatccgg caccgaaaga accgtctcca ccacgcgcga gccggatgcg
     1981 atccggagca cgtccgatcc ctttatgtag agcactccat gctccgtcac gaccgcgtcg
     2041 gccggagcgt cgccggacgc gaggcgaatc ggctcgtccg ccttgccgtc tggatcgatc
     2101 cgccaggcga acgttcccgt gctctcgatc accgggcgca ttccgccgta gggcaccggc
     2161 ccgttcgtcg ggagcagcca gggatagtgc gactcgaacg ggaccacctt caacacgctg
     2221 ccgaacgccc agatctcgcg gcatgatgcc gcgacgacgc tcacactcgc cgttccttcg
     2281 gtgccgggat ggggaagcgc cacggcagcg ggtttgcctc cggggcggta ccgcgtgagc
     2341 gcgccgcctt gaatcgccca caccgatccg gttccggccg cgatgtccga tgtcgcgttg
     2401 cgtccacgct caatgcgcac cgagccgcgc agcggcggat cgacgggcgg acactgcgac
     2461 gcgtccaccg gctcgacgcc gccgatcgaa ccggcaacca cgaccgaccc ggccgcgacg
     2521 agcgccgcga ccacggtcgc cagcacggtc gacccggcgc ggcgtcgcct cgcgcggcgc
     2581 tgtaccgcgg ccggaagctc gccggggtac ggcgcgcgct gcgcggcggc tgcaagctga
     2641 tcgcgcaggc gatcttcgat gctcatggct cgatcactcc tcgcagcgag gctaggcctc
     2701 gcgataccaa cgacttcacc gtgccgggac ggcagcccag gatgtcggcc gtctgacgct
     2761 cggacaggtc ctcgtagaac cgcagcacca gcgcggcgcg ctgccgcgcc ggcagccggg
     2821 tcatcgcgcg ccagagctcg tcgccgggcc ccggctcggc cggcatgacc accggaggtc
     2881 gcgtgtttcg ctcgcgccac tcacgctcga tccggtgccg gcggccgatg gacgcggcct
     2941 ggttgacgat cgcccgcttc aggtaggcgg gaaaggcgtc ctcgctgcgc agatcggtga
     3001 accgtgcggc catccgcacg atcgcgtcat ggaccagatc ctcggcctcc gtcgtgtcgc
     3061 cggtcagcag ataggcaaaa cgcagcgcgc cgggagcgtg acgctcgtag agcgccgcca
     3121 gccggctgcg cgccttctcg tgggactccg cctctgccac ctcggcccga atcatcacgc
     3181 atccaagacg gtttgcccca gcgttcggtt gcagtcgatt ccgcgctccc ctattcgtcc
     3241 gatacccttc gcttcagacc gcgaggagga acgcaatgga tttcagcgtc gcacccgagt
     3301 tggaagaggt ccggtcgtcg tatcgctcgt tcctcgagcg cgaggtgcgg cccgtcgaga
     3361 accggttcgc gcagcagctg cagggagacg agtggacgcc cgagatgcgc gaggccggga
     3421 tggagctgcg ccgcaagtcg gccgagatcg gctactacgg cgcgcacctg cccgaggacg
     3481 tgggcggctg gggcctgtcc acgctcggca tgactttgct ggtggaagag accggacgca
     3541 gcggcctgcg gttcgcgacc tacgtgctcg gcccgccgaa tcccgaggca cccacaccgc
     3601 tgctgctgga aggcaccccc gagcagcaga agaagtatct ggtgccgctg atcaaggccg
     3661 agaagaccat gtgcttcgcg ctgaccgagc ccgatgcggg cagcgatgcg cagtcgatcc
     3721 gcacgcgcgc ggtcaaggac ggcgatcact acgtcatcaa cggcgtgaag catttcatca
     3781 ccaacggcga gcgcgccgac ttcgcggtcg tgttcgcggt caccgatccc gacaagcgcg
     3841 cgcagggcgg catcaccgca ttcatcgtcg agaaaggcac gcccggctac acggtcggca
     3901 agaagcagcg caccatggcg ggcgactcca accagtcgga gctgatcttc gaggactgcc
     3961 gtgtgccggc cgcgaacatc ttggggccgg aaggctacgg cttcttcagc gcgatgaagt
     4021 ttctcaacgc cggccgcgcc tacatcggcg cgatgtgcct gggcatctcc gactacctga
     4081 ttcgcatctg caccgagtac gcgaacgagc gcaaggcgtt cggcaagccg atcggcaagt
     4141 ttcaggcgat ccagtggatg ctggccgatt cggctgtcga agttgaggcc gcgcgctggc
     4201 tgacctaccg gctcgcatgg atgtgtgatc aaggcgagca gccgatgcgc gaatcgtcga
     4261 tggtcaagct ctacaacacc gagatggtgg gccgcgtcgc cgaccgcgcc gtccagatct
     4321 acggcggcat gggctatctg accgaaggtc cggtcgagcg aatcttccgc tgggtgcgca
     4381 tgcttcgcat cgtcgaaggc accagcgaga tccagcgtct ggtcatcgcg cgaacgctgg
     4441 gcctgtgagc gaccacatcg cacgcaaccg cgcgacttgg gccgggttcg cccccgacta
     4501 cgtgcacaac gggcgcgtgt cctgggcgcg cgacgaaccc aagtggggcg tgttcggcat
     4561 cgacgacgac cgcatcctcc cggccggcat cgaaggcaag gacgtcgtcg agctgggctg
     4621 cggcaccgcg tacgtgtcgt cgtggttcgc gcgccgcggg gcgcgcagcg tcatcggcct
     4681 ggacctgacc ggcgagcagc tggacacggc gaatgcgctt cgcgcagagc acgggctgcc
     4741 ggtcgcgctg gtgcagggca acgccgaatg cacgccgttt cccgacgcat cgttcgacat
     4801 cgccttcagc gagtacggcg cgagcctgtg gtgcgacccg gccgcgtgga ttcccgaagc
     4861 agcccgcatc cttcggcccg gcggcgagct gacgtttctg accaacggca tcttcatcga
     4921 ggtctgcatt cccgacaccg acggcgaagg gccggcgacg gaccgaatgc tgcgaccgta
     4981 cttcggcctg ggccggaccg agtggcccga agagccgggc gtcgagttcc acctgaccca
     5041 cggcgactgg attcgcctgc tgcgcgcgag cggcttcgag atcctggatc tgatcgagat
     5101 ccgcgtcccg gccgatgcca cgacgtcgtc gccctgggtc agcaccgatt gggcaaccaa
     5161 atggcccgtc gaagaggtct ggaaagcacg aaagcgataa ccttctgctc gtgctgttcg
     5221 atatcaacta tgcgctggtg ggggaaacgg ccgtcgcata cacggtcatc ggcgacggtc
     5281 ccgtcgatct gctgatcgca ccgccggtca actcccacct ggacatccaa tccgagctgc
     5341 ccgaatgggt tgagttccat cagcggctcg gccagttcgc gcgcgtgttc gtcttcgaca
     5401 agcgcgggac cggcctgtcc gatccgctgc cgggcaccgg cgtttcgacg ctcgaagagc
     5461 gcgtggacga catgctcgcg atcatggagc atgccgggtc ggagcgggtt gcggtcatgg
     5521 gctacggcga aggcggcgcg gccgcggcgc tgttcgccgc gacgcatccg gaccgaacct
     5581 ctcacctgat cctgctgaac accaccgcgt gcctgcgacg ccgcgacgac tatccgtggg
     5641 gatgggaccc ggtcgatacg ccgatggcga actggctggc gaacttcggc aaagaggagc
     5701 cgctgctgaa ggcgcggctc ggctcggcgt tgagcgatcc ccggattcgc atgtgggcga
     5761 cccgctgggt tcgctccagt tcgtcgccgt tgctgtaccg cgcgctgcag aaggtcgcga
     5821 tggatatcga cgtgcgcaaa gtgcttcccg ccatcagcgc cccgacgctg gtcatgcact
     5881 gcaccgacaa ccacattttg gatccgcaga acgggcgctt cttggccgcg cacatagccg
     5941 gcgcccgctt cgtcgagctt cccggcgacg agcactaccc gttcctcggg gacaacgccg
     6001 cgctgatcgc agagatcgaa gagcatctga cgggttcgcg caccgtcgaa gaagccgagc
     6061 gcgtcctggc aacggttctg tttaccgaca tcgtggattc gacgcagacc gccgcgcgca
     6121 tgggcgatcg cgaatggaag acgctgctga cccgccacca cgaggccgtg cgggacgaga
     6181 tcgcacgcca tcgcggccgc gagatcgcca ccgccggcga cggatttctg gccaccttcg
     6241 acggtccggc ccgcgccgtc cggtgcgcct ggaacgtgca caaggccgtc gagccctacg
     6301 gcatccggct gcgcgccggc ctgcacaccg gcgagatcga gctgcacggc gacacgatcg
     6361 ccgggatcgg cgtgcacatc gcggcgcgtg tcgcatcgat cgccggcgcc ggcgaagtgc
     6421 tggtgtcacg caccgtcagg gatctggtcg cgggctcggg catcagcttc gtcgaccgcg
     6481 gacggcacac gctcaaaggc gtgccggacg agtgggagat ctatgccgcc cagcattagg
     6541 catccgggac gtcggtgccg gtgtgctctc gaaccgccat ccatgcgatc cgccggtcac
     6601 cgcggaacac gaccaggtga tccggcgcga acgatacgtg cacgcggtcg ccggggcgga
     6661 acaggttctg cgaggaatgc accgacgaca cgacgcggcc cgacggcaga cgcacgaccg
     6721 agacgttgac cggcccctga aagcggcgct cgatgacctc ggcggtcgcg ccgccgtttc
     6781 cgttcgcgag cacgatgtct tcggggcgca gcatcagctc ggcttcgccg ggccggacct
     6841 cgccgggcgc gaacgcgaac gatcccagtt ccgaatcgat ccggtcggcc gtcaccgtgg
     6901 cggggatgaa ctcggcccgg ccgacgaagt cggcgacgaa gcgcgtttcg ggaacgtgat
     6961 agatctcttc gggccggccg agctgctcga tgcggccggc gtgcatgacg gcgatccggt
     7021 cggcgatgtc gaatgcttcg ctctgatcgt gcgtgacgaa cacggcggtc gcgccggcgg
     7081 cgcggatgat cgtgcgaacc tcgccgcgta cgcgcgcccg caggtccgca tccaggttcg
     7141 agaacggctc gtcgagcagc acgatcgccg ggcgcggcgc cagcgcacgc gccagcgcga
     7201 cgcgctgctg ctgccctccg gacagctcgt gcggataccg gtcgccgagc ccctccagcc
     7261 cgaccagtcg catgacctcc tcgacccggc cgcgttcatc gcgcgccacg ccgtagccga
     7321 cgttggccgc gacggtcatg tggggaaaca gcgcgtagtc ctgaaacacc agcggcgcgc
     7381 gacgccgctc gggcgccacg aacgcatccg gtccgtccac gacccggccc tccagctcga
     7441 ccaccccggc gtcggcgcgt tcgaagccgg cgatcacgcg caggaaggtc gtcttgccgc
     7501 agccgctggg acccagcaac gccacgatct cgccggcggc gatcgtcagc gtcacctcgt
     7561 ccaacgcgac gacgtttccg aagcgcttcg cgagcccgcg gcacgcaacc gcagacgcct
     7621 gttcgacggt catgcgaagc agcgtatcca tcacggccgc tcccgcagtc ggcgctcgcc
     7681 cggtccgctc aacagcaaag cggccagcgg cccggatgcg agcacgatca gcagcagcgc
     7741 cggcagcgcc gcccggccga acgatgcggc gttcgtcgcg ccccagacct cggtcgccag
     7801 cgacgaaaag ccggtcgggg ccaggagcag cgtcgcgggg agctctttca tcgcggtcag
     7861 gaacaccagc gccaggccgg ccagcacgcc gggacgcacc agcggcatca cgatgcgcag
     7921 cgcggtgcgg cgccggctgc tgcccagcga ctgcgccgct tcttcgacgt gcggatcgat
     7981 ctgaagcagc ccggttcgga gcgcgccgcc ggcctgcggc aggaacagca ccgcgtaagc
     8041 gaacgccagc atcgcgatcg tctggtagag cacgggaacg tagcgtgcgc cgaagaacac
     8101 cagcgccagc gcgaccacga tgcccggcag cgcgtacccg acgtaggtcg tacgctccaa
     8161 cgcgcgcgcg ccgcgtcccg gaaagcgcac gcccagcacc gcgaccggcc atgccgcgat
     8221 cagcgcgacg accgcgccca atcccgcgac gcgaaggctg ttccaagccg cggtcaccgc
     8281 gacgcgcacc ggctcgcctt ctgcggcagc ccgcacgagc cacagcacga tcaccgcgac
     8341 gggcagcagc agcgcgaagc ccgcgaccag cgcgcaggcg gcgacggccg gccaacgcca
     8401 gcggccaagc ggcatcaccg gagcaggccg ccgcccgccc ggatgccggc gatagaacgc
     8461 cgcgcgtccg cgtgcccgcg cttccagcac cagcacgatc agcgtgagcg cgaccagcat
     8521 caagcccagc actgcggcac gtgaccggtc gaacgacgcc tgatagctga cgtagatcgc
     8581 gcgcgtgaac gaatcgaacc gcagcagcga caccgcgccg aagtcgtgca gcgtatacag
     8641 cgcgaccagc agcgagcccg cgacgatcgc gggccgaagc tgcggcagca cgacgcgtcg
     8701 cagcgtaaaa cgccggccgc gacccaagcc gtagctggct tcctcgagcg ccggatccag
     8761 cccgcggatc gcggcccgca cgatcagcag cacgtacgga taggtgaaca gcgtcagcac
     8821 cagccacgcg ccgccgaacc cgtagatcga cggcaggcgc tgcacgccca gcggctcgag
     8881 ccagccctgc accagcccgc gcggccccag tgccgcgatg aagacgtagc cgcccacgta
     8941 gctggggatc accagcggca gcgccgtcag caccgtccag atccgccggc ccggcagatc
     9001 cgtgcgggtg gtcagccacg ccagcggaac cgcgatcgcg accgaggtcc ccgtgaccgc
     9061 gatcgccagc agcgtcgtgc gcaccagcag cccgaccgtg cgtgcatcgg tcacggtccc
     9121 ggcgataccg tccggcccca ggctccacga gcgcacgacc agatacaccg cgggaagcgc
     9181 gacgccggcg gcaaccgcga acccggccgc gccgagccat cgcggaatcc gccggcgcga
     9241 ggcgcgacgc tccggccggc gacggatggc cggtgctgcg cgcgtcatcg tcgccgtcga
     9301 acgtcccctt acaggatgcc ttcgtcgcgc agcatcgtca gcgtcgcctc gagatccgac
     9361 agcttcgaca gatcgaggtc gggcggctcg agcgccgaaa gcttcggcag atccgcagac
     9421 gcttcgacgc ccgtcgccag cggatactcg aacgtctcgg ttgcgaagaa ctgctgcgcc
     9481 tcggcggaca gcagatactt cacgaacgtg gacgcggccg ccttgctctt cgaggtgctc
     9541 agaattccgg cgcccgcgac gttgaccagc gcgcccgggt cgccgccttt gaagaagtga
     9601 ttcgcggcct tcatggccgg ctgctctttc ttcagccgaa gcaggtaata gtggttgacg
     9661 aagccgacct ggatctcgcc cgcggcggcc gcttccacga tcggcgtgtt cttgggatac
     9721 acgaccggat cgttggcctt gatgccgcgc agccatgtgc gcgcggcctc gtcgcccttg
     9781 agcacacgca ggccggtgac gaacgcttgg aaggatccgt tcgtcggcgc ccagccgatc
     9841 ttgcccttcc acttcgggtc ggtgaagcct tcgatcgatg ccggaaggtc ggcgggtttc
     9901 accgaatcgg tgttgtggac gaccacgcgt gcgcggcccg agatcccgac ccactttcct
     9961 gcatccgatc ggaaggcctc cggcaccgag ctcaagatct cttggtcgag gtttgcgagc
    10021 ccgttcgcga ccgcacccag cgcgccggcg tcctgcgcga agaacacgtc ggccggcgag
    10081 ttgccaccct cttcgagcat ctgcgccgca agctcggccg aatcgccgta ccgcacggcc
    10141 agcttgatcg acgggttggc ggcctcgaac ttttcgaaca gcggctggac taggtccttt
    10201 tcgcggcccg aatagacggt cagcgtggtc tgtccgccgt cgccgccggt ccccccgggc
    10261 tcggaggctc cgcaggcggc ggcgaccagc gcaagcagga cggcggccag ggccgtcttg
    10321 gatccgtaac gcattcgttc tccccttttg cggcggcgcc accgctggcg gcagcgcctt
    10381 gacatcactt gtaaaggcat ccttacagac caattcctac tcgctggatc gggttttcgc
    10441 aacccgtggt cgcgttaggc tcggtcgcga tggccgaaac cgatctgctg cgaaagatcc
    10501 cccaggttga cgcggtcctg cgcgacgacg ccgtccgcgg ggtggctgcc ctgctgggcg
    10561 aggccgccgc cgcccgcttg gtccgcgatg cgatcgccgc cgcccgggcc gcggcgcaga
    10621 agggcaccga gccgccctct gccgccgcaa tggccaccga gatcgcggca cgggccacgg
    10681 cgctgcacgg ctcgcgcctt cgccgggcgg tcaacgcgac cggcgtgatc cttcacacca
    10741 acctcggccg cgcgccgctg tcgcgcgagg cgatcgcagc ggtcgcggat gcggccgggc
    10801 cggcgccgct ggagttcgac atcgccgccg gcgagcgcgg agtccgcgcg ccgctggccg
    10861 cgttcctggc cgcgacgctg accggtgccg aggcggcgca cgtggtcaac aacaacgcgg
    10921 ccgcgctgct gctgatgctg gccgcgatcg cgcgcgaccg cgaggtgatc gtcgcacgcg
    10981 gcgagctgat cgagatcggc ggcgagttcc ggctgccctc gatcatggaa gcgtccggcg
    11041 ccatgctgcg cgaggtcggc acgaccaacc gcacgcatcc gcgcgactat cgcggcgcga
    11101 tcggcgaacg caccgccgcg atcctgctgg tgcatccgtc gaactatcgc gtcgtcggct
    11161 tcaccactca gccgtcgctg cccgacatcg gcgcgatcgc acgcgaagcg ggcgtgccgc
    11221 tgctctacga catcggcagc ggcctgctcg cgcccgacga gcaccttccc gacgagcccg
    11281 atgcgcgcgg cgcgctggac ggcggcgcgg atctgatctg cttttcgggc gacaagctgc
    11341 tcggcggacc gcaggccggc gttctggcgg gccgaaagga tttggtggcc gcctgcaaac
    11401 ggcatccgat cgcccgcgcc gtgcgcgcgg acaagatgac gctggccgcg atggaggcaa
    11461 ccttgcttca gatcgcgcgc ggcgcgccga cgcccgtcgc ctcgatgctg tcggccgaca
    11521 cggaaacgct ccggagccgc tgcgaatcgc tggcgtccgg gctggaaggc gcgagcgtga
    11581 tcgaaggcca gagcgtcgcc ggtggcggca gcgttcccgg ccacggcatc gcgtcaccgg
    11641 tcgtcgcgat cgcctgcgac aaacccgagc gtgtcacggc cgcgcttcgc gcgaacgatc
    11701 cgccggtgat cgctcgcatc gacgacggac ggttgatcgt ggatccccgg accgtcgatc
    11761 cgtccgacga cgccgcgctt cgcgcggcgc tggagcgcgc tattcgctct tgaccttgcg
    11821 ctccagctcg gggtgcgagg cgtcggtgaa ctcgcggaac tcgccggtca gaagaaacgc
    11881 gacctgctcg ccgatgtcca ccgcgtgatc gccgacgcgc tcgagctgac gcgacacgat
    11941 catgatccgc aggccccact cgagcaggcc cgggtcgctg gcgcaggccg cgacctcggc
    12001 gaccatgccg cggttaatgc ggtcgatggg atcatccatc tgcggaagct gcatcgccag
    12061 cgcctggtcg cggttctgga aggcggtcag cgcggccgag accatgcgct cggcgatcgt
    12121 ggccatctcg ccgatgctgt ccaggatcga ggcgcgggtg ggcagctcgt cggtcagctt
    12181 gacggccttg gcgatgttga cggccatgtc accgatgcgc tccaggtgga ggttgatgtg
    12241 gatcatcgcc gacagcaggc gaaggtcgga tgcgaccggg gtctgcagcg ccagcagctc
    12301 cagcgtgcct ttctcgatcg acatgtagag gcggtcgacc tcgtcgtcct cgtcgatgac
    12361 ctgatcggca agggccccgt cgcgcgccag aatggcgcga acagacttgt tcagcagcgc
    12421 cagggcgcgc tcgccttcca ggacgaggtt cgtctcgagc gagcgaagtt catcgtggaa
    12481 gtgcctgcgc ggttcggtca ccgggcctcc ttttcggcca ttctacgcct cgtcccggcc
    12541 ggagccggtt ttgggatctt cgaacttgta tccgaggccg cgaaccgtct tgatgtagcg
    12601 aggcgagcgc gggtcggact cgatcttctg acgcagccgc ttgacgtgca catccagcgt
    12661 tctcgtgtcc ccgacgtact cgggccccca cacctcgtcg atgagcgtct gtcgcgtcac
    12721 cagacgcccg gccctggaca tcagggagtg cagtagctcg aattctttcg gcggaagctc
    12781 gaggcccgcc ccgtccacgc ggacctcgtg ggcgtcgggg tccagcgtga ctccgccgat
    12841 cttgagcgcg accgcccccg gcgagaccgg gacggatccg cgtcgcgaga ccgcgcggac
    12901 ccgcgcgatc agctcacgca tcgagaacgg cttggtgacg tagtcgtcgg cgccgagctc
    12961 cagacccagc accttgtcgg cttcggagtc cttggccgtg aggatgatga tgggaaccgg
    13021 ggatcgggcg cggatcgcct tgcacacgtc cagccccgaa acctcgggca gcatcaggtc
    13081 cagcaggatc acgtcggcac cgccgccgcg aaacttctcg agcgcttcct tgccatcccg
    13141 cgcgaggctg acgcggaagc cttcccgctc gagcccgtag cggacggctt cccgcagggt
    13201 ctcttcatcc tcgaccagca ggatcttcat gccgcgcctc cggccggcag cacgagcacg
    13261 aaccgcgatc cgcggccgaa ctcgctggac gcttcgacgc ggccgccgtg tcgctcggcg
    13321 acgtgcttga cgatggccag ccccagaccg gtcccgccgg tggcgcgtgc gcgggccttg
    13381 tccacgcggt aaaaccgctc gaagatgcgg ccgagctcct cggacgggat gcccggccct
    13441 tcgtcggcga ccgtgaacga caccacgcgg ccggtcgacg ccacggccag cagcaccggc
    13501 gtgcctgcgg gggcatagcg gaccgcgttg tcgagcaggt tcgccagcgc ggtcgccgcc
    13561 gcgcccgcat cggcccgcac gagcgcccgg tccggctgct ccaggctgat cgccgtgcct
    13621 ttcgcctcgg cgaaggccga gactcgctcg atggcgtcct cgacgatcac gctcgccggc
    13681 gtgtcggcga tcgcaagcgg catctcgctc tcgatgcgcg acaggtcgag cagatcggcg
    13741 acgaggcgag cgagccggtc cgtctcggtt tggatgcgc
//
LOCUS       DATLFO010000232        13660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87339, whole genome shotgun sequence.
ACCESSION   DATLFO010000232 DATLFO010000000
VERSION     DATLFO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87339"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="ftsY"
                     /locus_tag="VM841_09370"
     CDS             <1..1141
                     /gene="ftsY"
                     /locus_tag="VM841_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HVL90427.1"
                     /translation="AWAAPPAAAPPKPEAPPAEPKAEAPPAVEAPASPPPPAPVSPAP
                     PAPAPPKPEAPGAPTPAPPLEAPPAAEVPLVEEVEEVVEPKKRLRERLFKSRSGFGQA
                     LVGVFKRSQLTEEDWEDIEAILLQADVGVNATMKIVEDLRDNAKKVAPDELVGKLREN
                     LLGIIGDGERRMIRKSDGLTVWIVTGVNGTGKTTTIGKLALRLHREGKKVVLAAADTF
                     RAAADEQLQRWAERSGAELVRHQHGADPAAVAFDGVKAAKSRGADVLIVDTAGRLHTK
                     TNLMEELSKIRRVVEREAGAPDEVLLVLDATTGQNGIVQARAFLEAVGVTSVALTKLD
                     GTAKGGIVIAVQQELGLPVKLVGVGETPEDLQDFDPAAFVDALIS"
     gene            1165..2580
                     /gene="ffh"
                     /locus_tag="VM841_09375"
     CDS             1165..2580
                     /gene="ffh"
                     /locus_tag="VM841_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HVL90428.1"
                     /translation="MFDSLTDRLEAAFRRLRGSGRLSEDDVDEVLKEVRMALLEADVH
                     LQTVRHFVSRVRERAVGEEVLKTLTPAQQVVKIVNEELVGILGGESEPLRFSQRPPTI
                     IMMTGLQGSGKTTASGKLAMQIKKGGRRPLLVACDLRRPAAIQQLQQLGEQIGVPVVA
                     DESATDPVALAKSSLEVARQQGRDVIIVDTAGRLHVDEEMMHEAAAIRDTINPTETLL
                     VVDAMTGQDAVNVATSFLEKVDYSGIILSKLDGDARGGAALSIAHVSGRPVKFASVGE
                     KLDEFEPFHPDRMASRILGMGDVLTLIEKAEEAYEEEEAAALEEKMRKGKFDLQDFLD
                     QMKQLKKMGSLGGLLKMIPGMRQHMKGLGEIDDDALKHTQAVILSMTPAERAEPGVID
                     ASRKKRIAKGCGMTVNDVNALLRQFDQAKQMMKAMVGGGVPGVPAMTGGGGAGKSGSG
                     KAKRPHRPKTHHKNKKKKRKK"
     gene            complement(2661..3041)
                     /locus_tag="VM841_09380"
     CDS             complement(2661..3041)
                     /locus_tag="VM841_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90429.1"
                     /translation="MRRIALAALALALAGQGSLSAKPGDPPESCQGVVAQRGMCLLIA
                     PGEGPVTYSVSGTAYGATEARIQIRLEDALTGKALVECSADRTGSPVVDVTCQRSATV
                     EDPPLVSRCVAEGAIAGRFFCDAI"
     gene            3145..3429
                     /gene="rpsP"
                     /locus_tag="VM841_09385"
     CDS             3145..3429
                     /gene="rpsP"
                     /locus_tag="VM841_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVL90430.1"
                     /translation="MSVRIRLTRKGKKGQPFYRVVVADQRSPRDGRFVEEIGTYNPLT
                     DPSTININEEKALAWLAKGAQPSEQVGQLLRRVGIWQKFTATKKKTTKAS"
     gene            3381..3680
                     /locus_tag="VM841_09390"
     CDS             3381..3680
                     /locus_tag="VM841_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVL90431.1"
                     /translation="MAEVHGHEEEDDEGVVAATLEYVARALVDHPDSVSVTRHPGEPR
                     PVVRLDVHPDDIGHVIGRGGRMARSIRAVAKAAAALDDLSVHVQIGEPRDRGDSR"
     gene            3683..4198
                     /gene="rimM"
                     /locus_tag="VM841_09395"
     CDS             3683..4198
                     /gene="rimM"
                     /locus_tag="VM841_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005187313.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HVL90432.1"
                     /translation="MAGARRLVVGRIVTAHGIRGECVVDVLSDAPDRFDPGRTVQIDE
                     PDGRRMLTVEAARPHKARLLVLFSEVPDRTAAEALRGRWLTIPAEDAAPLPEGRFYPH
                     QIEGFDVRDEAGAPLGVLESVLEYPAHDIWVVRTPDRREVMVPAVAEFVRGVDPEAKS
                     IVLAPIEGMFE"
     gene            4195..4878
                     /gene="trmD"
                     /locus_tag="VM841_09400"
     CDS             4195..4878
                     /gene="trmD"
                     /locus_tag="VM841_09400"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVL90433.1"
                     /translation="MRIDVITIFPEFFGSPLRTSLLGKAVERGTVAVNVHDLRDHATD
                     KHRTVDDEPFGGGAGMVMKPGPWFAAVEAIDGWEQARRILLTPAGRRLDHGLAVSLAA
                     TPHLILMCGRYEGIDERVAMLATDEVSIGDFVLAGGESAALIVIEAATRLVPGVVKEQ
                     ASVEEDSFATGLLDYPHYTRPASFRGMAVPDVLLSGDHGRIERWRRDEALRRTKARRP
                     DLLPPGEDG"
     gene            5049..5399
                     /gene="rplS"
                     /locus_tag="VM841_09405"
                     /pseudo
     CDS             5049..5399
                     /gene="rplS"
                     /locus_tag="VM841_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            5867..6658
                     /gene="lepB"
                     /locus_tag="VM841_09410"
     CDS             5867..6658
                     /gene="lepB"
                     /locus_tag="VM841_09410"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVL90434.1"
                     /translation="MQAPPVAPSPASGPVPGDPDASHAGPESAPEDEKPTGKKQGSFF
                     KELPILILIAFGLALLIKSFLIQAFYIPSESMVPTLNVGDRVLVNKVVYKMREPRRGE
                     VIVFVGERFQENRSFLQRVRDALTSGFGGKQGPERDFIKRIIGLPGETVEGVGGRVIV
                     TPAGGGASFALDEPYLAQPMTQSFSAVTVPKGSYFVMGDNRGNSSDSRGALGSIKKGE
                     IVGKAFVRVWPLSRFRRLTRPGYAEGAAIALPALWMHHRRRRDAA"
     gene            6662..7462
                     /gene="lepB"
                     /locus_tag="VM841_09415"
     CDS             6662..7462
                     /gene="lepB"
                     /locus_tag="VM841_09415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVL90435.1"
                     /translation="MPTAPPLEQETFTSAWPQAPAGPPPPPRQRFGLMRELPVLIVVA
                     LTVALLIKTFLIQAFYIPSASMVPTLLENDRILVNKLAYRFREPERGEVVVFITGNLL
                     RERRSLVQAAWKNVCEALGLCPPGDTDFIKRIIALPGETIEVKDDPNAGHRAVYITTP
                     NGRTFILPEPYIESYGELQAFGPFTVPAGSYFLMGDNRGNSSDSRINNFGICPSAPCA
                     VPKERLVGKAFVRIYPFKRLHVFQVPVYGLTVMGVAGGVVRRRRRRAD"
     gene            7509..8132
                     /locus_tag="VM841_09420"
     CDS             7509..8132
                     /locus_tag="VM841_09420"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVL90436.1"
                     /translation="MEPDGTLERSLSTAGFRLVAGVDEVGRGALAGPLYACAVILPDD
                     LDVDGIKDSKLMTAKARERVAAEVRERALAVSIVRVMPSSIDRRGLHPSNLWALRQAI
                     VRLAPAPDYVLSDGFRPKRLPMPALSIKKGDAVARSVAAASVLAKVARDAAMRRLARR
                     YPGYGFERNVGYGTAEHWDALRRLGPTPVHRRSFAGVAEPTPEEMAT"
     gene            8129..8443
                     /locus_tag="VM841_09425"
     CDS             8129..8443
                     /locus_tag="VM841_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HVL90437.1"
                     /translation="MSLDDLEKYEADLELALYREYRDVVGMFNYVVETERRFYLSNKV
                     EVTPRSNGEDSYFEILLTDAWVWDIYRPARFVASVRVISYEDINVEELRNDIPDSPEG
                     LD"
     gene            8633..9001
                     /locus_tag="VM841_09430"
     CDS             8633..9001
                     /locus_tag="VM841_09430"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HVL90438.1"
                     /translation="MNCTDRNALGRAGEDAAASYLAYMGWTIVARNVRRREGEVDIVA
                     RRAGTLAFVEVKTRRSAAFGTPAEAVTIRKQRRIRTLAAVLLAEGIGHARLVRFDVID
                     VRPCRCSPGCFTVDHIEDAF"
     gene            8998..10512
                     /locus_tag="VM841_09435"
     CDS             8998..10512
                     /locus_tag="VM841_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HVL90439.1"
                     /translation="MSFARATSVAVVGMDGHAVEVEVHVARGLPAFTVVGLPDAAIQE
                     SRERVRAAVESSGESWPKQRLTVNLSPAHLPKQGSGFDLAIALAILAACGRIPQQRLE
                     QVCAIGELSLDGGVRRVRGALASVASCAARGVPRVLVPQGNAAEASLIDGVEVIPLSA
                     LRDAVGLLRGERRIDAAVPPPTVTGGPGEVDLAEVRGQAHAKRALEVAAAGGHNMIMT
                     GTPGGGKTMLARRLPTILPPLDRTEAMAVTRLYSVAGLLPPDAGLIVARPFRAPHHSI
                     SVAGLVGGGSGVPLPGEVSLAHRGVLFLDEATEFRRDALQALRQPLEDGTVRLSRARY
                     SVTYPSRVQLVLAANPCPCGHAGDVARACECPPGRIVAYRERLSGPLLDRIDIGVVVE
                     RVSKSQIFSRGRSEPSTAVRARVEAARAVQGARLAPIGIATNAEIPPRALDDACALTR
                     DARTAVSRAVETYGLSMRGAHRLMRVARTIADLEDSAEVGAGHVTEAAGLRLRR"
     gene            10512..11453
                     /locus_tag="VM841_09440"
     CDS             10512..11453
                     /locus_tag="VM841_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVL90440.1"
                     /translation="MRSRPRHRVPDGLFERASGGGVRARVPLRELTPDDPEFPEGVRT
                     MPRAPSRLWVAGRDLRTLGSMVAVIGARRATRYGLSMAAGLAGDLAAGGVCVVSGLAR
                     GCDSAAHDGALERGGTTVAVLGSGADVCYPAGSRVLYSEILAAGAVVSARPPGYRPFK
                     SDFVYRNSIMVAMSEAVVVVQGTTDSAAVRTGEDAAGAGHGSVFAVPGSVEWPESAGV
                     HKLLKLGATVCTSASDIVEPLQDRLRWTPPELQPVPRHLPEPQRRILEALAGGAAGRE
                     EVVAASALEAAAASRAIAELEMAGLIVEEGFFLRRTR"
     gene            11547..12092
                     /locus_tag="VM841_09445"
     CDS             11547..12092
                     /locus_tag="VM841_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90441.1"
                     /translation="MRKLGFLATAGAGAILMLAVAPGMASAHHLNPFGDIVVEADRDA
                     EVSDRVFVPVGGGHGDVSVLRQGGNVVFGLEAFGDVVIGREAPGHGVFVARGGGHGDV
                     LVDEILSNETLPEAAAARRATGTANSCYQGACADVTFVEAGIDAGETFGGGAHSYGPQ
                     QAIRTGALDTTGVVIAGSFIF"
     gene            12199..13185
                     /locus_tag="VM841_09450"
     CDS             12199..13185
                     /locus_tag="VM841_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HVL90442.1"
                     /translation="MSRAWESEARDAVEAFLRHSALERNLSPHSVAALRADLEQFLGF
                     CGHTGGPRASGPLGAKPHHLQRFLASLNERLDPDELAVVSVWRSRAEPYARTSLARKA
                     STLRSFYAFAQRRGLREDNPAVLLVSPKRHQRLPKVLRRPQAAALIDTPPGDDPVGLR
                     DHAVLELLYGAGLRVGELCSLDLEAVDVTRRRVRVMGKGAKERIVPFGEQAAAAIASY
                     VAEGRPHLVRDRTPAGALFLNRRGNRMGQRDVRSLVTRYAAEAAPGSMPSPHTLRHSY
                     ATHLLEGGADLRSVQELLGHSDLRTTQIYTHVSRERLRRVYEQAHPRAGEDG"
     gene            13185..>13660
                     /locus_tag="VM841_09455"
     CDS             13185..>13660
                     /locus_tag="VM841_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL90443.1"
                     /translation="MAQQRQKAAVDPEEASAIQQLWDRFKASGDEDARERLIIHYSPL
                     VKYVAGRVSVGLPNTIEQADLTSYGIFGLIDAIEKFDPSRNIKFETYAINRIRGAIID
                     ELRAVDWIPRSVRSKAREVERALAVGGRTVQLALELGERPLDLARLGAHAARNPVHG"
BASE COUNT         2079 a         4742 c         4796 g         2043 t
ORIGIN      
        1 ggcttgggcg gcgccgcccg cggccgctcc gcccaagccg gaggcgccgc ccgccgagcc
       61 gaaggccgag gcgcccccgg cggtcgaggc gccggcttct cccccgcctc ccgcgcccgt
      121 ttcgccggcg cctcccgcgc ccgctccgcc caagccggag gcgcctggcg cgcccacgcc
      181 cgctccgcct ctcgaggctc ctccggccgc ggaagtcccc ctcgtcgagg aagtcgaaga
      241 ggtcgtcgag ccgaagaagc ggcttcgcga gcggctcttc aagtcacgct cgggcttcgg
      301 tcaggcgctc gtcggcgtct tcaagcggtc gcagctgacc gaagaggact gggaggacat
      361 cgaagcgatc ctgctccagg ccgacgtggg tgtgaacgcc acgatgaaga tcgtcgaaga
      421 cttgcgtgac aacgccaaga aggtcgcgcc cgacgagctc gtcggcaagc tgcgcgagaa
      481 cctgctcggc atcatcggcg acggcgagcg gcgcatgatc cgcaagagcg acgggctgac
      541 cgtgtggatc gtcaccggcg tcaacggaac gggcaagacc accacgatcg gaaagctcgc
      601 gctgcgcctg caccgggaag gcaagaaggt cgtgctggcc gcggcagaca cgttccgggc
      661 cgccgccgac gagcagcttc agcgctgggc ggagcggagc ggcgccgagc tggttcgcca
      721 tcagcacggc gcggacccgg ccgcggtcgc cttcgacggc gtcaaggccg ccaaatcgcg
      781 cggtgccgat gtgctgatcg tggacacggc cgggaggctg cacaccaaga ccaacctgat
      841 ggaagagctc agcaagatcc ggcgcgtcgt cgaacgcgag gccggcgcac ccgacgaggt
      901 gctgctcgtc ctcgacgcca ccaccggaca gaacgggatc gttcaggcgc gcgcatttct
      961 tgaggccgtc ggcgtcacgt cggtcgcgct caccaagctc gacggcaccg ccaagggagg
     1021 catcgtgatc gcggtgcagc aagagctcgg cctgccggtg aagctcgtcg gcgtcggcga
     1081 aacgcccgaa gaccttcaag atttcgatcc tgccgccttc gtggacgcgc tgatctcgta
     1141 gcgccccggt agaatccctc gcctatgttc gattccctta ccgatcgcct ggaagcagcg
     1201 ttccgccgcc tgcgtggcag cggccgtctc agcgaggatg atgtcgatga ggtcctcaaa
     1261 gaggtgcgca tggcgctcct cgaggccgac gtgcacctgc agaccgtccg gcacttcgtg
     1321 tcgcgggtgc gtgaacgcgc ggtcggcgaa gaggttctca agacgctcac gcccgcgcag
     1381 caggtcgtca agatcgtcaa cgaagagctc gtcgggatcc tgggcggcga gtccgagccg
     1441 ctgaggttct cgcagcggcc gccgacgatc atcatgatga ccggcctcca gggctcggga
     1501 aagaccaccg cttcgggcaa gctggcgatg cagatcaaga agggcggccg ccgcccgctg
     1561 ctggtcgcgt gcgatcttcg ccgccccgcc gcgattcagc agttgcagca gctcggcgag
     1621 cagatcggcg taccggtcgt tgccgacgag tccgcgaccg atccggtcgc gctggcgaag
     1681 tcgtcgctgg aggtcgcgcg gcagcaggga cgcgacgtga tcatcgtgga caccgccggc
     1741 cgtctgcacg tcgacgaaga gatgatgcac gaggccgccg cgatccgcga cacgatcaac
     1801 ccgaccgaga cgctcctggt ggtggacgcg atgaccggtc aggacgcggt gaacgtcgcg
     1861 accagcttcc tcgagaaggt ggactacagc ggcatcatcc tgtcgaagct cgacggcgac
     1921 gcgcgcggtg gcgccgcgct ctcgatcgcg cacgtgtccg gccgtcccgt gaagttcgcc
     1981 tccgtgggcg agaaactcga cgagttcgag ccgttccacc ccgaccgcat ggcgtcccgg
     2041 atcctgggca tgggcgacgt gctgacgctc atcgagaaag ccgaagaggc ctacgaggaa
     2101 gaagaggccg cggcgctcga agagaagatg cgcaagggga agttcgacct gcaggacttc
     2161 ctcgatcaga tgaagcagct caagaagatg ggttcgctcg gcggtctgct caagatgatt
     2221 cccggcatgc gccagcacat gaaggggctg ggcgagatcg acgacgacgc gctcaagcac
     2281 acgcaggccg tcatcctttc gatgacaccg gccgagcgcg ccgagccggg cgtgatcgac
     2341 gcctcacgca agaagcgcat cgccaagggt tgcggcatga ccgtcaacga cgtcaacgcg
     2401 ctgctgcgcc agttcgatca ggcgaagcag atgatgaagg cgatggtcgg cgggggagtg
     2461 cccggcgtgc ccgcgatgac gggcggaggc ggagcgggca agagcggcag cggcaaggcc
     2521 aaacgtcccc accggcccaa gacgcatcac aagaataaga agaagaagcg caagaagtag
     2581 gccgggctcc ggatcgcgcc ggcggccccc ctcgtcaggt ttccagacgc acgccgcgtc
     2641 aggtccctgg acgcaccgcg tcagatcgcg tcgcagaaga agcggcccgc gatcgcgcct
     2701 tcggccacgc agcggctcac gagcggcgga tcctcgaccg tggcgcttcg ctgacaggtc
     2761 acgtccacga cgggtgagcc cgtccggtcg gccgagcatt cgacgagcgc cttgcccgtc
     2821 agcgcatcct cgagccggat ctggatccgc gcttcggtcg cgccgtaggc cgtcccggat
     2881 accgagtacg tgaccgggcc ctcgccgggc gcgatcagca ggcacatgcc tcgctgggcc
     2941 acgacgccct ggcagctttc cggagggtcg ccgggcttgg ccgacaggct cccctgcccc
     3001 gccagggcca gggcgagcgc ggcgagcgcg attcgtctca tgacggctct ttcgcgccgc
     3061 cccgcggacg gtcctgcctt cgaatccgac tttccgggcg gctgtgtggc ggagtgagcg
     3121 gacctccggt agcctgtgcg gcctatgtca gttcgcattc gtctcacgcg caagggcaag
     3181 aagggccagc ccttctatcg cgtcgtcgtc gccgaccagc gttcgcctcg ggacggccgt
     3241 ttcgtcgagg agatcgggac ctataacccg ctcaccgacc cttcgaccat caacatcaac
     3301 gaagagaagg cgcttgcctg gctcgccaag ggcgctcagc cctccgagca ggtcggccag
     3361 cttcttcgcc gggtggggat atggcagaag ttcacggcca cgaagaagaa gacgacgaag
     3421 gcgtcgtagc agcgacgctg gaatacgtcg ctcgcgcgct cgtcgatcac cccgactccg
     3481 tttcggtcac ccgtcatccg ggtgagcctc gccctgtcgt gcgcctggac gtgcaccccg
     3541 acgacatcgg ccacgtcatc ggtcgcggcg ggcgcatggc ccgttcgatc cgcgccgtcg
     3601 cgaaggccgc cgccgcgctc gacgacctga gcgtgcacgt tcagatcggc gagccgcggg
     3661 accgcggcga ctcgcgctag cgatggccgg cgctcggcgc ctcgtcgtcg gccgcatcgt
     3721 caccgctcac gggatccgcg gtgaatgtgt cgtcgacgtc ctgagcgacg cccccgatcg
     3781 cttcgacccc ggccggaccg tccaaatcga cgagcccgac ggccggagga tgctcacggt
     3841 cgaggcggcg cgtccgcaca aggcccgcct gctggtgctg ttctccgaag tcccggaccg
     3901 caccgccgcc gaggcgctgc gcggccggtg gctgaccatc cccgccgagg acgccgcccc
     3961 gcttcccgaa ggccggttct acccgcatca gatcgaaggc ttcgacgttc gcgacgaggc
     4021 cggcgcgccg ctgggggtgc tcgagagcgt gctcgagtat ccggcccacg acatctgggt
     4081 cgtgcgcacg ccggatcgcc gggaggtcat ggtccccgcg gtcgccgagt tcgtgcgggg
     4141 cgtcgatccc gaagccaagt cgatcgtgct cgcgcccatc gaggggatgt tcgaatgagg
     4201 atcgacgtca tcacgatctt cccggagttc ttcggctcgc cgctgcggac ctcgctgctg
     4261 ggcaaggcgg tcgagcgcgg gaccgtcgcg gtgaatgtgc acgacctgcg cgatcacgcc
     4321 accgacaagc atcgcacggt ggacgacgaa ccgttcggcg gcggggccgg catggtgatg
     4381 aagcccgggc cctggttcgc ggcggtcgag gcgatcgacg gatgggagca ggcccgccga
     4441 atccttctga cgcccgcggg ccgccggctc gatcacggct tggcggtgtc gctcgccgcc
     4501 accccgcacc tgatcctgat gtgtggccgc tacgagggca tcgacgagcg cgtcgccatg
     4561 ctcgcgaccg acgaggtctc catcggagat ttcgtcctgg ccgggggcga gtccgcggcg
     4621 ctgatcgtga tcgaggcggc cacgcgcctg gtgccgggcg tggtcaagga gcaggcctcc
     4681 gttgaagagg actccttcgc caccggcctg ctcgattatc cgcattacac gcgaccggcc
     4741 tcctttcggg gcatggcggt cccggatgtc ctgctcagcg gcgaccacgg gcgaatcgag
     4801 cgctggcggc gggacgaggc cctccgtcgg accaaggccc gccggccgga cctgctgccg
     4861 cccggcgagg acgggtagat cccggatcga tcccggggcc ggaagcgacg gtgactgccg
     4921 agaccggacg gggccgaagc gagcgggatc gtccgggaga ggcgggttgc tacggcggaa
     4981 agccctggta acatggtccg tcgcgtctgc cgaacgatct ccggcgacgc tcgtcgtaag
     5041 gagaccctgt gaacaagatc gacctggttg agcagccacg cctgcgcacc gacatcccgg
     5101 cgttccgtcc cggggacacc gtcaaggtcc acgtcaaagt cagcgaaggc gggcgcgagc
     5161 gcatccaggt cttccagggc gtcgtcatcg cccgtcgcgg tggcgggatt cgtgagagct
     5221 tcacggtccg caaaatctcc ttcggcgtcg gagtcgagcg cgtcttcccg ctgcatacgc
     5281 cgactatcgc caagatcgag atcgtccagc acggcaaggt ccgccgcgcc aagctctact
     5341 acctgcgcga gcgcaccggc cgtcacgcca agatccgcga gcgccgcatc atggctccgg
     5401 tggaaggcac cgccgtcgct cccgaggaca tcgtgcccga gatcgcggat gaggcgcccg
     5461 ccgagatcgc ggctccggaa gcggcagccg agcccgtggc tccggaagcg gccgccgggg
     5521 acgcgcccgc agccgaggca gccgccgagc ccgcccccga ggcgaccgag ccggcccctg
     5581 cagcgagcga gccgggtgcc gaatccggtg cagagtcggg cgacggccaa gaggccgcgc
     5641 ccgaggcgca gccggaagag cccaaggcct aggtaacatc ccctccgccg tgagcagcga
     5701 ctccgacggc ggaacgatcc cgacaggatc gcagccgccg actcctccgc cgcaaccttc
     5761 cgaggttggc ggatccgcat acccacccga cgggccgcag catcccgcgg agccccgtca
     5821 tcccgacggg ccgcagcatc ccgcggagca cgcgcatccc gcggggatgc aagcacctcc
     5881 cgtcgcgccc tcgcctgcct ccgggccggt cccgggggat cctgacgcga gccatgcggg
     5941 gcccgagtcc gcgcccgagg acgaaaaacc cacgggcaag aagcagggct cgtttttcaa
     6001 ggagctgccg atcctgatcc tgatcgcctt cggattggcg ctgctgatca agtcgttctt
     6061 gatccaagcc ttctacatcc cgtccgagtc gatggtcccg accctcaacg tcggggatcg
     6121 cgtcctggtc aacaaggtcg tctacaagat gcgcgagccg cggcgaggcg aggtcatcgt
     6181 cttcgtgggc gagcggttcc aggagaaccg ttcgttcctg cagcgcgtcc gcgacgcgct
     6241 cacgagcggg ttcggcggca agcagggtcc cgagcgcgac ttcatcaagc gcatcatcgg
     6301 gctgcccggc gagaccgtcg agggcgtcgg cggccgcgtg atcgtcacgc cggcgggcgg
     6361 cggggcgtcc ttcgcgctcg acgagcccta cctcgcgcag ccgatgaccc agtcgttctc
     6421 ggccgtgacc gtcccgaagg gctcctactt cgtgatgggc gacaaccgag gcaactcgtc
     6481 cgactcgcgc ggtgcgctcg gctcgatcaa gaagggtgag atcgtcggca aggcgttcgt
     6541 gcgggtctgg cccctgagcc ggttccgtcg cttgactcgc cccggctatg ccgaaggagc
     6601 cgcgatcgcg ttgccggcgc tgtggatgca tcaccgccgc cggagggacg cggcgtaggc
     6661 catgccgacc gcaccgccgc tcgagcagga gacattcacc tccgcgtggc ctcaggcgcc
     6721 cgccggtccg ccgcccccgc cccggcagcg cttcggcctg atgcgcgagc tgcccgtcct
     6781 gatcgtggtg gcgctcacgg tcgccctgct gatcaagacg tttctgatcc aggccttcta
     6841 catcccgtcc gcgtcgatgg ttccgaccct gttggagaac gaccgcatct tggtcaacaa
     6901 gctcgcctac cggttccggg agccggagcg gggcgaggtc gtggtgttca tcaccgggaa
     6961 tcttctgcgt gagcgcagga gcctggtcca ggccgcctgg aagaacgtgt gcgaggcgct
     7021 gggactgtgc ccgccgggtg acaccgactt catcaagcgc atcatcgcgc ttcccggaga
     7081 gacgatcgag gtgaaggacg acccgaacgc ggggcatcgc gccgtctaca tcaccacgcc
     7141 gaacggcagg acgttcatcc tgcccgagcc ctacatcgag tcctacggcg agctgcaagc
     7201 ctttggcccg ttcaccgttc ccgcgggcag ctacttcctc atgggagaca atcggggcaa
     7261 ctcctccgac agcagaatca acaacttcgg gatctgcccg tcggcgccgt gcgcggttcc
     7321 gaaagagcgc ctggtcggca aggccttcgt acgcatctat ccgttcaagc gcctgcacgt
     7381 gttccaggtg cccgtgtacg gcctgacggt catgggcgtg gccggcggcg tggtccgccg
     7441 acgccgccgg cgcgcggact gaccgccgga tgcggccatt cggccggggg cggcggtatt
     7501 ctcgcagtgt ggaacccgac ggcaccctgg agcgctcgct ttcgacggca ggcttccgtc
     7561 tcgtcgccgg cgtggacgag gtcggacgcg gcgcgctggc cggcccgctc tacgcctgcg
     7621 ccgtcattct gcccgacgat ctggacgtcg acggcatcaa ggactccaag ctgatgaccg
     7681 ccaaggcgcg cgagcgcgtc gccgccgaag tgcgtgagcg cgccctggcc gtgtcgatcg
     7741 tgcgcgttat gccctcctcg atcgaccgac gcggcctgca cccgtcgaac ctgtgggcgc
     7801 tgcggcaagc gatcgtgcgc ctggcgccgg cgccggacta cgtcctgtcg gacgggttcc
     7861 ggcccaagcg gctgccgatg ccggcgctgt caatcaagaa gggcgacgcg gtcgcgcgca
     7921 gcgtcgccgc ggcgtccgtg ctcgcgaagg tggcccgaga cgcggcgatg cgccgcctgg
     7981 ctcgccgcta tccgggatat ggcttcgagc gcaacgtcgg atacggaacc gccgagcatt
     8041 gggacgcgct gcggcgtctc ggtcctaccc cggtgcaccg gcgctcgttc gccggcgtcg
     8101 ccgagccaac gcccgaggag atggccacat gagcctcgac gatctcgaaa agtacgaagc
     8161 cgatctcgag ctcgccctct accgcgagta ccgcgacgtc gtgggcatgt tcaactacgt
     8221 ggtcgagacc gagcgacgct tctacctctc gaacaaggtc gaggtcacgc cccgcagcaa
     8281 cggcgaggac agctatttcg agatcctgct gactgatgcg tgggtctggg acatctaccg
     8341 gccggcgcga ttcgtggcca gcgtgcgggt gatctcctac gaggacatca acgtcgagga
     8401 gctgcgcaac gacatccccg actcgcccga agggctcgac tgacgtggga tacaacccgc
     8461 ttcgcaagta ccgcggcggg aaggctgccg aggtcgcgat cctcgtggtg ttcacggcgg
     8521 tgctcatcgg cctgatcgtg tgggccgtgc gctaatccgt cggccaagcc gtccgcttct
     8581 gctcgatctt cccgcacggg acgtgcctcc gcgcagcccc tagcgtgcgt tcatgaactg
     8641 caccgaccgc aacgcgctcg gccgcgcggg tgaggacgcc gcggcgtcgt acctggcgta
     8701 catgggctgg acgatcgtcg cgcgcaacgt gcggcgacgc gaaggcgagg tcgacatcgt
     8761 cgcccgccgc gccggaaccc tggcgttcgt cgaggtcaaa actcgccggt cggctgcgtt
     8821 cggaacgccg gccgaggcgg tgaccatccg caagcagcgc cgcatccgaa cgctcgcggc
     8881 cgtgttgctg gccgaaggga tcggtcacgc gcggctggta cggttcgacg tgatcgacgt
     8941 ccgcccgtgc cggtgttcgc ccgggtgctt caccgtcgat cacatcgagg acgcgttttg
     9001 agcttcgcgc gcgcaacctc ggtcgccgtc gtcggcatgg acggccacgc cgtcgaggtc
     9061 gaggttcacg tcgcgcgcgg gctgccggcc ttcacggtcg ttggcctgcc ggacgccgcc
     9121 attcaagagt ccagggagcg ggtacgggcc gcggtcgagt ccagcgggga gtcctggccg
     9181 aagcagcgtc tgacggtgaa cctttcgccc gcgcacctgc cgaagcaggg cagcggcttc
     9241 gatcttgcga tcgcgctggc gatcctcgcg gcgtgcggcc gtattccgca gcagcggctc
     9301 gagcaggtct gtgcgatcgg cgagctttcg ctggacggcg gggtgcgccg ggttcggggc
     9361 gcgctcgctt cggtcgcgtc gtgcgcggct cgcggcgttc ctcgcgtgct ggtgccgcag
     9421 ggcaacgcgg cggaggcgtc gctcatcgac ggggtcgagg tgatcccgct gtcggcgctg
     9481 cgcgacgcgg tgggtctttt gcgcggcgag cggcgcatcg acgcggcggt gccgccgccg
     9541 accgtgacgg gcgggccggg cgaggtcgat ctggccgagg tccggggcca ggcgcacgcg
     9601 aagcgcgcgc tggaggtggc cgccgccggc gggcacaaca tgatcatgac cggaaccccg
     9661 gggggaggaa agacgatgct ggctcgccgg ctccccacga tccttccccc gctcgaccgg
     9721 accgaggcga tggcggtcac gcggctgtat tcggtcgccg gcctcctccc gcccgacgcc
     9781 gggctgatcg tcgcacggcc ctttcgggcc ccgcaccatt ccatctccgt cgcggggctg
     9841 gtcggcggtg gcagcggagt gccgttgccc ggcgaggtgt ccctggccca ccgcggcgtg
     9901 ctattcctcg acgaggccac cgagtttcgc cgtgacgcgc ttcaagcgct ccggcagccg
     9961 ctcgaggacg gcacggtccg gctctcccgg gcccggtact cggtgacgta cccgtcccgg
    10021 gtgcagctcg tcctggctgc caacccgtgc ccgtgtggcc acgcggggga tgtcgctcgc
    10081 gcctgcgaat gtccgccggg acgcatcgtc gcctaccgcg agcggctgtc ggggcccctg
    10141 ctggaccgca tcgacatcgg cgtggtcgtc gagcgcgtga gcaagagcca gatcttctcg
    10201 cgcggccgct ccgagccctc gacggcagtg cgggcgcgcg tggaggcggc gcgcgccgtg
    10261 caaggcgcac ggctcgcgcc catcgggatc gccaccaacg ccgagatccc tccgcgagca
    10321 ttggacgacg cctgcgcgct gacgcgggac gcccggacgg cggtgagccg ggccgtcgag
    10381 acctacgggc tgtcgatgcg cggcgcgcac cggctgatgc gcgtggcgag gacgatcgcg
    10441 gatctggaag actcggccga ggtcggagcc ggacacgtga ccgaagcggc cggcctccga
    10501 cttcgacggt gatgcgcagc cggcctcgtc accgcgttcc cgacggtctg ttcgagcgcg
    10561 cctcgggcgg cggggttcgg gcccgcgtcc cgctccgcga gctcacgccc gacgacccgg
    10621 agtttcccga aggggtgcga acgatgccgc gcgcgccgtc gcggctgtgg gtcgcgggcc
    10681 gggatctgcg aacgctcgga tcgatggtgg ccgtgatcgg agcgcggcgc gcgacccgat
    10741 acggactctc catggcggcc gggctcgcgg gcgacctcgc cgccggcggg gtgtgcgtgg
    10801 tcagcggcct ggcccgcgga tgcgactcgg ccgcgcacga cggggcgctc gagcgcggag
    10861 gcaccaccgt cgcggtcctg ggttcgggtg ccgacgtgtg ctatccggcg ggcagccgcg
    10921 tcttgtactc ggagatcctc gccgccgggg ccgtggtctc ggccaggcct cccggctacc
    10981 ggccgttcaa atccgacttc gtctaccgga actcgatcat ggttgcgatg tcggaggccg
    11041 tcgtggtcgt gcaggggacc accgacagcg ccgcggtcag aacgggcgag gacgccgcgg
    11101 gcgccgggca cgggtccgtc ttcgccgtcc cgggatccgt cgagtggccc gagagcgccg
    11161 gcgtccacaa gcttctcaag ctcggagcca cggtctgcac ctccgcatcc gacatcgtcg
    11221 agccgctcca ggaccggctt cgctggacgc cgcccgagct ccagccggtc ccacgccatc
    11281 tgcccgagcc gcagcgccgg atcttggagg ccctggccgg cggggccgcc ggcagggagg
    11341 aggtggtggc ggcgagcgcc ctggaagccg cagccgcctc ccgggcgatc gccgagcttg
    11401 agatggccgg cctgatcgtc gaagagggct ttttcctccg ccgaacccgg tgattcttgc
    11461 cacccggcga agtcggccat acgatccaat ctccgaaatt caccgataag ccccatagcc
    11521 tccgatcggg ggcgagagga ggacggatgc gaaagcttgg ttttctcgcg accgcaggag
    11581 caggagcgat cctgatgctg gcggtcgcgc ccgggatggc atcggcccat cacctgaacc
    11641 cgttcggcga catcgtcgtc gaggccgatc gggatgccga ggtgtccgac cgggtcttcg
    11701 tgccggtcgg cggcgggcac ggcgatgtga gcgtgctccg gcagggcggg aacgtcgtgt
    11761 tcgggctgga ggcgttcggt gacgtcgtta tcggacgcga ggcgccgggt cacggtgttt
    11821 tcgtggcgcg gggcggcgga cacggcgacg tgctcgtgga cgagatcctg tcgaacgaga
    11881 ctcttccgga agccgcagcc gcgcgtcggg cgacgggcac ggccaacagc tgctaccagg
    11941 gcgcatgcgc cgacgtcact ttcgtcgagg ccgggatcga cgccggcgag accttcggcg
    12001 gcggagccca ctcgtacgga ccgcagcagg cgatccgcac gggcgcgctg gacacgaccg
    12061 gcgtcgtcat cgccggcagc ttcatcttct aggcacatct cgagacgctc ggagcccggc
    12121 ggtcgccggg ctccgcgctt tccacgctcg gttcgttgac cgcgccgctg cccggcacga
    12181 tagtgcctcg tgacacgaat gagccgggcg tgggagagcg aggcaaggga cgcggtcgaa
    12241 gcgttccttc gccacagtgc gctcgaacgc aacctgtcgc cgcacagcgt ggccgccttg
    12301 cgcgcggacc tggagcagtt cctcggcttc tgcggccata ccggggggcc gcgagccagc
    12361 ggcccgctcg gggccaagcc gcaccatctg cagcgcttcc tcgcctcgct caacgagcgt
    12421 ctggatcccg acgagctcgc cgtggtttcg gtctggcgct cccgcgccga gccctacgcg
    12481 cgcacgagcc tggcgcgaaa ggcatcgacg ctgcggtcct tctacgcgtt cgcgcagcgg
    12541 cgggggctgc gcgaggacaa ccccgcggtg ctgctggtct cgcccaagag acatcagcgt
    12601 cttccgaagg tgctgcgtcg cccgcaggcg gccgccctga tcgacacgcc cccgggcgac
    12661 gacccggtgg gccttcgcga ccacgccgtc ctcgagctgc tctacggcgc cgggcttcgc
    12721 gtgggcgagc tgtgctcgct ggacctggag gcggtggacg tgacccggcg gcgcgtgcgc
    12781 gtgatgggaa agggagccaa ggagcgaatc gttccgttcg gtgagcaggc agccgccgcg
    12841 atcgcctcat acgtggcgga aggccgcccc catctggtcc gggaccgcac cccggccggg
    12901 gccctgttcc tgaatcgccg tggaaaccgg atgggtcagc gggacgttcg ctcgctcgtg
    12961 acccggtatg ctgcggaggc ggctccgggc tcgatgccgt cgccgcatac gttgaggcac
    13021 agctatgcga cgcatctgct tgaaggaggc gcggacctga gatccgttca ggagcttctc
    13081 ggccattccg atctgcgcac cacgcagatc tacacgcacg tgtcccgtga gcggcttcgc
    13141 cgcgtctacg agcaggccca cccgcgcgcc ggggaggacg gctgatggcc cagcagcgcc
    13201 agaaggctgc cgttgatccc gaggaagcgt cggcgatcca gcagctgtgg gaccggttca
    13261 aggcatccgg cgacgaggac gcacgcgagc gcctcatcat ccactactcg ccgctggtca
    13321 agtacgtcgc cggccgtgtc tccgtcggct tgccaaacac catcgagcag gcggacctga
    13381 ccagctacgg catcttcggg ctcatcgacg cgatcgagaa gttcgacccg agccgcaaca
    13441 tcaagttcga gacgtatgcg atcaaccgga tccgcggcgc gatcatcgac gagcttcgcg
    13501 ccgtggactg gattccgcgc agcgtgcgct ccaaggcgcg cgaggtcgag cgggcgctcg
    13561 ccgtcggtgg gcggacggtg cagctcgctc tggagctcgg cgagcgcccg ctcgacctcg
    13621 cgcgccttgg agcgcacgct gcgcggaatc cagtccacgg
//
LOCUS       DATLFO010000233         7163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_123427, whole genome shotgun sequence.
ACCESSION   DATLFO010000233 DATLFO010000000
VERSION     DATLFO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123427"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VM841_09460"
     CDS             complement(<1..501)
                     /locus_tag="VM841_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HVL90444.1"
                     /translation="MRGVRVRRDERGAAAIVVALSLVVVMAAAMLTIDAGMLWNQRRT
                     IATATDAAALAEARRAALTPGAATACGSQWVTMLSRNTRSQADALSCTVLPGDRGASF
                     EVRARLASPVGFGAALGVRDAEAFASSVARFDPVSLVLEKTRPIAVCTQNTHMQEWFA
                     YLDGTIP"
     gene            complement(489..977)
                     /locus_tag="VM841_09465"
     CDS             complement(489..977)
                     /locus_tag="VM841_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HVL90445.1"
                     /translation="MLSRRPRRGDDGSAAVEFAIVGSLLFLILLGIVQFGIAFNRSQG
                     LQAAAREGARIASLPASTSAAIQQRIRNTVSIVDGTNLSPACPANPSSLPADRGCIRI
                     QRRSSGGALITVNGAAARPCENQTGATVLITVFYRSEITVPLWKSQKVTLSGEGEFAC
                     EA"
     gene            complement(971..1162)
                     /locus_tag="VM841_09470"
     CDS             complement(971..1162)
                     /locus_tag="VM841_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL90446.1"
                     /translation="MKGSRPRRSVFERREDGATSVEYALLLFLITAVIIVAAFTFGDN
                     ASELYMCTQETVTTKVPSC"
     gene            complement(1258..1488)
                     /locus_tag="VM841_09475"
     CDS             complement(1258..1488)
                     /locus_tag="VM841_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL90447.1"
                     /translation="MTIFRQIRTFAQFVTARLDLKSEKGATAVEYGLMIALIAAVIIA
                     AVVVLGNNAKDQFQCTADSIAARADNCAPAAP"
     gene            complement(1654..2160)
                     /locus_tag="VM841_09480"
     CDS             complement(1654..2160)
                     /locus_tag="VM841_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL90448.1"
                     /translation="MSRLSSRRILVVEDEPHVRMLTSMVLEDAGFSIDQAADGSEALD
                     ALRARSYAAIVLDIMMPGVDGYQVLEEMQQMRNRRGTPVVVVTARHDPSGVLRELSGG
                     AFDHLAKPYTHEDLIAAVHRAIEGDDVEERRGALWRSAQAWDTVGVLRNAVTENDGAP
                     APPRRRRR"
     gene            complement(2157..2804)
                     /locus_tag="VM841_09485"
     CDS             complement(2157..2804)
                     /locus_tag="VM841_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90449.1"
                     /translation="MNTTTVLMLDDHTLVRQSLVKTVSAEEGFDVVADSGDGDEAVAL
                     AARHRPHVAVLDVSLGGASGLDIAVRMKDKAPEMRLIFLSMHDDDATIHRALRIGADG
                     YVVKTASTDELLIALRSVSAGGSYLSPHVARRVMEFASGPTTGRLTDRELEILGLMAS
                     GERISEVASTLFVSTKTVKNHLTSIYAKLGVSSGGQAIAEAYRRGLVPTPIGGSS"
     gene            complement(2801..4324)
                     /locus_tag="VM841_09490"
     CDS             complement(2801..4324)
                     /locus_tag="VM841_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVL90450.1"
                     /translation="MRRARPDRLVVFVAGCDEYTTLLVARHLPQARIATAESRRCPRL
                     AVVEAADAAAVAAVERCRAETIVAIADRQDPRLPFEALTVVRPFAPGVLFDAIDDQLQ
                     QPARDRYRPTLLAAIRLALITLGAGVRGFGDGAAPDLAFVSCLVLYGVARMVGTRFEH
                     GARAIDVGVACALVAATEGAHSGFVPFAALTVVAAGLAMGWRAGAVAGGLVSLAAGTP
                     EVIEAIVRRTPLTDALALLALFPLAGMTGGFAQRIHGAASSREKLAETNRLLTRLHGI
                     AREIPGAMDAQTVASAVVDELREMGAPAAAVLDDGTELSVRAGYGVAHEPIAAATSLR
                     ACGEIRILDVDAEPRPVREALAEHRHWIVAPAVRNGRFHGILLAAVGEAGAEATWRGA
                     LTRLAGDAAVAFENAELLRRVGALAADEERRRIALRVHDGLAQTLTHLRLELDFIVRH
                     DDDGDRIRREIQRLSHVLDRAIGDVHGLMHDLKTDRTDEEPPAIISAPDSGKERMPA"
     gene            4628..5131
                     /locus_tag="VM841_09495"
     CDS             4628..5131
                     /locus_tag="VM841_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90451.1"
                     /translation="MRRLAAVLVLIVAAGCSSNESGGPGAVSPTPTATATTTEPAQGN
                     VKGGSAKADVSGQETFSVEVPLSTGDSQFNAPPAGFAFVFKDDKDNLITIGGESFDGS
                     RPTAGTLALGVVFNREGRVIAHTDTSGGCEVTMKREGGAVSGTFQCGQIEDGRYSVKG
                     TFSAELV"
     gene            5509..6234
                     /locus_tag="VM841_09500"
     CDS             5509..6234
                     /locus_tag="VM841_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HVL90452.1"
                     /translation="MEPTRETVRPLLELQRIDSSVDRLNGRAAALPEQQEMDRLTEEL
                     AAARKTLAERELGFGGLSRDTAKLEAEVEALSDKIKHENDRLYSGSVGSPKELTAIQA
                     EIDGLRKRKNHVEDQLLELMEKKEAAEKDFNESKEQVSGLESQVAAATVARDTATTDI
                     AGEIEKLQDERRALLPSIPEEAVEVYEQVRPKKEGVAVAAYEHGVCRGCMVELSPVAR
                     DEIKRSDDPFPRCENCRRILIVL"
     gene            6234..6707
                     /locus_tag="VM841_09505"
     CDS             6234..6707
                     /locus_tag="VM841_09505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HVL90453.1"
                     /translation="MSSYAEIVAHTDGGARGNPGPAGIGVHLASSSGKTIDEIAEAIG
                     VATNNVAEYKAVIRALERSLELGAKRVTVRADSLLAVEQLRGVYKVKNAGLKPLHAAA
                     RALVAKFDSVTFEHVRREKNKKADALVNVAIDQWVDAYGDAPPIPEPDGPPTLFG"
     gene            complement(6696..6842)
                     /locus_tag="VM841_09510"
     CDS             complement(6696..6842)
                     /locus_tag="VM841_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90454.1"
                     /translation="MSERIIDADDLRFLVRDEGAGDAVLLLHGIPLDEPDELDALPLD
                     FLAE"
     gene            6871..>7163
                     /locus_tag="VM841_09515"
     CDS             6871..>7163
                     /locus_tag="VM841_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HVL90455.1"
                     /translation="MAVRSAALRQSPLAFLGLRPVLTAVLGACAIAFSGIFVRLAAES
                     PVTASVFRTAYALPALGLLAWIERRRFGPRARRDHRLALVAGVAFAADLILWH"
BASE COUNT         1102 a         2427 c         2561 g         1073 t
ORIGIN      
        1 cgggatggtc ccgtccaggt aggcgaacca ttcctgcatg tgcgtgttct gggtgcagac
       61 cgcgatcggg cgcgtctttt cgagcacgag cgaaaccggg tcgaaccgcg ccaccgacga
      121 ggcgaaggcc tcggcgtcgc gtacgccgag ggcggcgccg aagccgacgg gggaggcgag
      181 ccgcgcgcga acctcgaacg aggcgccgcg gtcgccggga agcacggtgc acgagagcgc
      241 gtcggcctgc gaccgggtgt tgcgcgagag catcgtcacc cattgcgagc cgcaggcggt
      301 cgcggcgcct ggcgtgagcg cggcgcgccg agcctcggcg agcgcggcgg cgtcggtcgc
      361 cgtcgcgatc gtacgccgct ggttccacaa catcccggcg tcgatggtca gcatcgccgc
      421 ggccatgacc acgacgagcg agagtgccac cacgatggcc gcggcccctc gctcgtcgcg
      481 cctcaccctt acgcctcgca tgcgaactca ccttctccgg acaaggtgac cttctgagac
      541 ttccacagcg gcaccgtgat ctcgctgcga tagaagacgg tgatgagcac ggttgcgccg
      601 gtctggttct cgcacggacg tgcggcggcg ccgttgacgg tgatgagcgc gcctcccgag
      661 ctccggcgct ggatgcggat gcaccctcgg tccgccggca gcgacgacgg gttggcgggg
      721 cacgccggtg acaggttcgt cccgtccacg atcgagaccg tgttccggat ccgctgctgg
      781 atggccgcgc tcgtgctcgc cggaagcgag gcgatcctcg cgccctcgcg cgcggcggcc
      841 tgcagaccct gcgaccgatt gaacgcgatg ccgaactgca cgatgcccag caggatcaga
      901 aacagcagcg agcccacgat cgcgaactcg acggcggcac tcccgtcgtc gccgcgtcga
      961 ggccgccggc tcagcacgac ggcaccttcg tcgttacggt ctcctgggtg cacatataca
     1021 gctcgctcgc gttgtctccg aacgtgaagg cggcgacgat gatcaccgcc gtgatgagaa
     1081 acagaaggag ggcatactcg accgaggtcg ctccgtcttc gcgtcgttcg aagacggagc
     1141 gtcttgggcg agatcccttc acgtctccct ccttcggcgg gttctgccgt cgtagcggga
     1201 gggctccggg cgggggcccg cccggagccc tcgtgtcata tcgatgcgag gccggcctta
     1261 cggcgccgcc ggggcgcagt tgtcggcgcg ggcggcgatc gagtcggcgg tgcactggaa
     1321 ctggtctttc gcgttgttcc cgagcaccac gaccgctgcg atgatcacgg cggcgatgag
     1381 cgcgatcatg aggccgtatt cgaccgcggt cgcgcccttc tcgctcttga gatccaggcg
     1441 tgccgttacg aactgtgcga acgtccggat ctgacggaag atcgtcatcg gcttggttca
     1501 cctcccttgg tcatccagcg gccggtggcc gcgtcgtgtt ccgttacgac gatgatcggc
     1561 gccgggaggt ggcttgccat gaggcagagg gcggcagtgc acgggtgcat ccgtcccggc
     1621 tcgccgggac gaaggtcccg gcgagccggc gtgtcagcgg cggcggcggc gcggcggcgc
     1681 gggcgcgccg tcattctcgg tgacggcgtt gcgcaggacg ccgaccgtgt cccacgcctg
     1741 tgcggagcgc cacagcgcgc cgcggcgctc ttcaacgtcg tcgccttcga tggcgcgatg
     1801 caccgcggcg atgaggtctt cgtgcgtgta tggcttggcg aggtgatcga acgcgccgcc
     1861 ggacagctcc cgcagcacgc ccgacgggtc gtgacgcgcg gtgacgacga cgacgggcgt
     1921 gccccgccgg ttccgcatct gctgcatctc ctcgagcacc tgatagccgt ccacgcccgg
     1981 catcatgatg tcgagcacga tggcggcgta ggagcgcgcg cgaagcgcgt cgagcgcctc
     2041 ggatccgtcg gcggcctgat cgatggaaaa gccggcgtcc tcgagcacca tcgaggtgag
     2101 catccggacg tgcggctcgt cttcgacgac gaggatgcgg cgcgacgaca atcgactcat
     2161 gaggagcctc cgatcggggt gggcacgaga ccgcgacggt atgcctcggc aatggcttgt
     2221 ccaccggagg aaacaccgag cttggcgtag atgctggtga ggtggttctt gaccgttttg
     2281 gtggacacga acagtgtcga cgcgacttcg ctgatccgct cgcccgaagc catcaacccg
     2341 aggatctcga gctcacggtc ggtcaggcgt ccggtcgtcg gaccggaggc gaactccatg
     2401 acccgtcgcg ccacgtgcgg gctcaggtag gagccacctg ccgacaccga tcgcagcgcg
     2461 atgagcagct cgtcggtgga cgcggtcttg accacgtagc cgtcggcgcc gatccgcagc
     2521 gcacggtgga tggttgcgtc gtcgtcgtgc atcgacaaaa agatcaggcg catctcgggc
     2581 gccttgtcct tcatgcgaac ggcgatgtcg aggccgctgg cgccgcccag cgatacgtcg
     2641 agcacggcga cgtgcggccg gtggcgtgcg gcgagcgcaa cggcttcgtc tccgtcgccg
     2701 ctgtcggcga cgacatcgaa tccctcctcg gccgagacgg tcttgacgag gctctgccgg
     2761 acgagcgtgt gatcgtcgag catcaggacg gtagtcgtgt tcatgcgggc atccgctcct
     2821 ttcccgagtc gggagccgaa atgattgcgg gcggctcctc gtcggttcga tcggtcttga
     2881 ggtcgtgcat caggccatgg acgtcgccga tggcccggtc cagcacatgg gacagtcgtt
     2941 ggatttcgcg gcgaatccgg tcgccgtcgt cgtcgtggcg cacgatgaag tcgagctcga
     3001 ggcgaagatg cgtcaacgtc tgcgcgaggc cgtcgtggac gcgcagcgcg atgcggcggc
     3061 gttcttcgtc ggccgcaagc gcgccgacgc gacgaagcag ctcggcgttt tcgaaggcaa
     3121 cggccgcgtc gccggcgagg cgcgtcagcg ctccgcgcca ggtggcctcg gccccggcct
     3181 cgcccaccgc ggccagcagg atcccgtgga agcgtccgtt gcgcacggcc ggggccacga
     3241 tccaatgccg gtgctcggcg agcgcctcgc gcaccggacg cggctcggca tccacgtcca
     3301 agatccgaat ctcgccgcag gcgcgaaggg aggtggcggc cgcgatcggc tcgtgggcaa
     3361 cgccgtagcc ggcgcgaacc gacagctcgg tgccgtcgtc gagcacggcg gcagccggcg
     3421 cacccatctc gcgcagctcg tcgacgacgg cgctcgcgac ggtctgcgcg tccatcgcgc
     3481 cgggaatctc gcgcgcgatc ccgtgcagac gcgttaggag ccggttcgtc tcggcgagct
     3541 tctcgcggga cgacgccgcc ccgtggatcc gctgcgcgaa gccgccggtc atgccggcga
     3601 gcggaaacag cgccagcaga gcgagcgcat ccgtgagcgg ggtgcggcgc acgatcgcct
     3661 cgatcacctc gggcgtaccg gccgcgagcg atacgagtcc gcccgccacg gcgccggccc
     3721 gccatcccat cgcgagcccg gcggctacca cggtcagcgc ggcgaacggc acgaagccgc
     3781 tgtgcgcgcc ttccgtcgcg gccacgagcg cgcacgccac gccgacgtcg atcgcacgcg
     3841 ctccgtgctc gaagcgcgtc ccgaccatgc gggcgacgcc gtagagcacg aggcaggaca
     3901 cgaaggcgag gtcgggtgcg gccccgtcac cgaagccgcg cacgccggcc ccgagcgtga
     3961 tcagcgcgag gcggatcgcg gcgagcaggg tcgggcggta ccggtcgcgg gccggctgct
     4021 gcagctgatc gtcgatggcg tcgaagagca cgccgggcgc gaagggccgc acaaccgtga
     4081 gcgcctcgaa cggcagccgt gggtcctgcc ggtccgcgat cgccacgatg gtctcggccc
     4141 ggcagcgctc gaccgccgcg acggcggctg cgtcggcggc ttccacgacg gcaaggcgcg
     4201 ggcaccgccg gctttcggcc gtggcgatgc gcgcctgcgg cagatgccgg gcgacgagaa
     4261 gcgtcgtgta ttcgtcgcac ccggcgacga acacaaccaa gcggtcggga cgagcccttc
     4321 gcatgatgtc cgatctcccc ggcccaccgg cgccggagcg ccttggggcc ttcgcggttc
     4381 ggaggtcccc aatggtgtcg gcatcctagg acgctggtca tgacaccaag agatggtttc
     4441 atcggcccct aaatgcaggg gctcggggct cgatcgcccg aggaatcgcg ggtgggtggc
     4501 cggctgatcg ccggatggtc gccggtggat cgccggtcgg tcacttgctc cggccgcctc
     4561 cgtcgcggtt gcgcgggccc cccgcatggg gtgaactggc cgggccgttc gatcgaaagg
     4621 aatccgcatg cgccgtctcg ccgcagtcct cgtcttgatc gtcgccgccg gttgcagcag
     4681 caacgaatca ggcgggccgg gagcggtgtc gccgacgccg acggccaccg caaccacgac
     4741 cgagcccgcc cagggcaacg tcaagggcgg ctccgccaag gcggacgtga gtggtcagga
     4801 gaccttctcg gtagaggttc ccctcagcac gggcgactcg cagttcaacg ctccgccggc
     4861 aggctttgcc ttcgtcttca aggacgacaa ggacaacctg atcacgatcg gtggcgagtc
     4921 gttcgacgga tcccgcccga cggcgggcac gctcgcgctc ggggtggtct tcaaccggga
     4981 gggccgcgtg atcgcgcaca ccgacaccag cggcggctgc gaggtgacca tgaagcgtga
     5041 gggcggcgcg gtgtccggca cgttccagtg cggccagatc gaggacggcc gctattcggt
     5101 caagggaacg ttttctgcgg agctggtgta gcgcgggccg aacggccggt tgcggtgaga
     5161 gccaccagac ggccgcggac agggcagggg agggcggacc ggcgggcccg gagtcttcag
     5221 cggtcttatc gccggtggct gtcggcgtag agcgccagcg cgctcaggcc gcccagcatc
     5281 acgacgatcg ttgcccagat catggccgct ccgttctcat ccgtccgtag atgtggcatc
     5341 ggcaccagtt tcatctgtct tgaacgaccg agccaccgaa tttgatgcac gccgggccca
     5401 aaaggttccc cgggtcctgg gggacgaaac cgtcccgggc gcgcgcggct atactgcggc
     5461 tcgcgccggc cgcccggccc gcgcccgacg ttgacaagaa ggagcctggt ggagccgacg
     5521 cgcgagaccg tgcgcccgct gctggaactt cagcggatcg actcctccgt tgaccggctc
     5581 aacggccgcg ccgccgcgct gcccgagcag caagagatgg accggctcac cgaggagctc
     5641 gccgccgcgc gcaagacgct ggccgagcgc gagctcggct tcggggggct cagccgcgac
     5701 accgccaagc tcgaggccga ggtcgaggcc ctgtcggaca agatcaagca cgagaacgac
     5761 cggctctaca gcggctcggt cggcagcccg aaggagctca ccgcgattca ggccgagatc
     5821 gacgggctgc gcaagcgcaa gaaccacgtc gaggaccagc tgctcgagct gatggagaag
     5881 aaggaagcgg ccgagaagga cttcaacgag tccaaggagc aggtctccgg gctcgagtcc
     5941 caggttgcgg ccgcgaccgt cgcgcgcgac accgcgacca ccgacatcgc cggcgagatc
     6001 gaaaagctcc aggacgagcg gcgcgcgctg ctgccctcga tccccgagga ggccgtcgag
     6061 gtctacgagc aggtccgccc caagaaagag ggggtcgcgg tcgcggccta cgagcacggc
     6121 gtctgccgcg gatgcatggt cgagctgtcg ccggtcgcgc gcgacgagat caagcgcagc
     6181 gacgatccgt tcccgcgctg cgagaactgc cgccggatcc tgatcgttct ctgatgtcct
     6241 cctacgcgga gatcgtcgcc cacaccgacg gcggcgcgcg cggcaacccg ggtcctgccg
     6301 gcatcggcgt gcatctggcg tcctcgtccg gcaagaccat cgatgagatc gccgaggcga
     6361 tcggggtcgc caccaacaac gtcgccgaat acaaggcggt gatccgtgcg ctggaacgtt
     6421 cgctggagct gggcgcgaaa cgcgtgacgg tgcgggccga ctcgttgctg gcggtcgagc
     6481 aactgcgggg ggtctacaag gtcaaaaacg cggggttgaa gccgctgcac gccgcggccc
     6541 gcgccttggt ggcgaagttc gactcggtca ccttcgagca cgttcgccgc gagaagaaca
     6601 agaaggccga cgcgctcgtc aacgtcgcga tcgaccagtg ggtggacgca tacggcgacg
     6661 ctccgccgat ccccgagccc gacgggccgc cgacgctatt cggctaggaa atccagcggc
     6721 agtgcgtcga gctcgtccgg ctcgtcgagc gggatcccgt gcagcaggag aaccgcatcg
     6781 ccggcgcctt cgtcccgcac caggaagcgc aggtcgtcgg cgtcgattat ccgctcgctc
     6841 atgccgcgac gataccgtac gctccgcggt atggccgtcc gttccgcagc gctgcgccag
     6901 tcgccgctgg cattcttggg gctgcggccg gtgctgacgg cggtgctggg cgcgtgcgcg
     6961 atcgcgtttt cggggatctt cgttcggctg gcggccgaat cgccggtgac cgcgtcggtg
     7021 ttccgcaccg cgtatgcatt gccggccttg ggtctgctcg cctggatcga gcgccgccgc
     7081 ttcggcccgc gcgcgcgccg cgaccatcgg cttgcgcttg tcgcgggcgt cgccttcgcg
     7141 gcggacctga tcttgtggca tca
//
LOCUS       DATLFO010000234         8606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_74620, whole genome shotgun sequence.
ACCESSION   DATLFO010000234 DATLFO010000000
VERSION     DATLFO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74620"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VM841_09520"
     CDS             <1..884
                     /locus_tag="VM841_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HVL90456.1"
                     /translation="STPIETRIGTAPGLIVEHGGGVVYAIPGVPAEMREMMSRAVLPD
                     LARRAGTTAVTRTRVVRVSGMAESSIAEALSPLWRSLDDAPVKMSYLAGGGEVRVRIT
                     AKALDAAAADALLDPVERSIRETLGTAVVGTGDATLESVVGDLLKEVRATLAVAESVT
                     GGGVAERITRIPGSSAYFAGGVVAYDPAVKTALLGVDPALIEMDGVVSERVALAMARG
                     VRERTGATVGLALTGAAGPDAHGGMPPGSVWIAVDDASASAARLVRYASDRAAVRGFA
                     TTAALNLLRLHLSEARR"
     gene            890..1429
                     /gene="thpR"
                     /locus_tag="VM841_09525"
     CDS             890..1429
                     /gene="thpR"
                     /locus_tag="VM841_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HVL90457.1"
                     /translation="MFVAVGLSDEARAEAARAIDDLRPVVAAARWVPPENLHATVAFI
                     GETPDADAVIEAVTRAASPFPPVRLVLEGLGAFPSWRRARVLWAGLTGEVAVLSAAAR
                     AVGEALAPIGYEPEDRPYTAHVTLARLRSPGPVAAASAAVGPVSFLAEGLGVYRSLLG
                     RPAARYERLALAPFSASRA"
     gene            1638..2684
                     /gene="recA"
                     /locus_tag="VM841_09530"
     CDS             1638..2684
                     /gene="recA"
                     /locus_tag="VM841_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVL90458.1"
                     /translation="MAEQDKALEAALGQIEKQFGKGSIMRMGENEKMRIGVIPTGSIA
                     IDQALGIGGMPRGRIVEIYGAESSGKTTLALHVVAEAQRAGGIAAYIDAEHALDPVYT
                     ANLGVNLDELLISQPDTGEQGLEIADTLIRSGAVDVVVIDSVAALVPRAEIEGEMGDT
                     HVGLQARLMSQALRKLSGNLSRSNTCAIFINQLREKIGVMFGSPETTPGGRALKFYSS
                     VRVDVRRIENLKDGAEVVGSRVRAKIVKNKTAPPFRQAEFDIIYGKGISKEGSLIDVG
                     VDLGLVKKSGAWFTYDGTQIGQGRENARQFLLDNVDVAYDLEKRIRDALGLVPQEGGE
                     PVVVPVEAPAEDEK"
     gene            2719..3309
                     /locus_tag="VM841_09535"
     CDS             2719..3309
                     /locus_tag="VM841_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HVL90459.1"
                     /translation="MSTKPGDGEASASPSSSFRGSRRRAGTWPSEDASTPQQRRRNAT
                     EVVLRMLTARERSEAEVRRALSKRGYDPDTIAVVVQEFISKNLLSDARFAEAFAEEAA
                     QRRGYGAAAIRFKLRGKGVSPELAARAAAVSPEDEEERARELAAKKVRAMTALSPDVR
                     YRRLAGALARRGYPADIVSRVAADLSRHEPRAPGDE"
     gene            3647..5230
                     /gene="rny"
                     /locus_tag="VM841_09540"
     CDS             3647..5230
                     /gene="rny"
                     /locus_tag="VM841_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HVL90460.1"
                     /translation="MALTIGVLIGLVIGGAIGYFIRHRAGLATGSAAEAKARSVLEEA
                     EKEAERARRDAVVAAKDEALRIREEADSEIKGRRAELDRRESRFEQRESHLQAREEDL
                     DRQAISLADRGRLLEEREEALRREREELEGRSRELTARAGDLEHQIERVAAMTSAEAK
                     SVLTKRIEDDAKREAMATVRELETRAREEAEARARRIVTLAIQRLASEQTAESTVAVV
                     SLPSDDMKGRIIGREGRNIRAFESVTGVNIIIDDTPEAVLLSCFDPVRREVGRITLEK
                     LVADGRIHPSRIEEIFDRASGEVEEEIRKAGEAAVLDVGITDMHPDLIRVLGRLQYRT
                     SYGQNVLKHLIETAHIAGVMAAELGLPPDQVRMVKRCGLLHDIGKAVTHEVEGSHALI
                     GAELARRLKEPEPVVHAIESHHGEVEQRTLEAVLAQTADQISGGRPGARRETLENYVK
                     RLEKLEEICTSYPGVEKTFAMQAGREVRVMVKPESLDDLACQVLARDIAKRIEEELQY
                     PGQIKITVVRETRSTEFAK"
     gene            5410..5667
                     /locus_tag="VM841_09545"
                     /pseudo
     CDS             5410..5667
                     /locus_tag="VM841_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
     gene            5821..6912
                     /gene="hppD"
                     /locus_tag="VM841_09550"
     CDS             5821..6912
                     /gene="hppD"
                     /locus_tag="VM841_09550"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HVL90461.1"
                     /translation="MSDDAFPVLGIDHVEFWTGNAKQSAYFYTRAMGFREIAFSGLET
                     GVRDRTTRVLQQGKIRFAISGALGPSSPVAAHVARHGDGVRDVALRVPDAEEAYRVAL
                     ERGAIDVHPPQVFEDEHGKVVRAAIGTYGETIHSLIQRDDYSGPFLPGFAAVADDRSE
                     GGLKIIDHIVGNVEIGTMNVWADYYAQVMGFQNLVHFRDDQISTEYTALMSKVMWDGS
                     GRVKFPINEPAEGKKRSQIDEYLDFYAGPGVQHLALLTNDIVATVTELRDRGVEFLRV
                     PDAYYEDVAERFSDLSEVDVEALKRLAILADRDEEGYLLQIFSKPVMDRPTVFFEVIE
                     RHGSRGFGLGNFKALFEAIEREQALRGTL"
     gene            6921..8030
                     /locus_tag="VM841_09555"
     CDS             6921..8030
                     /locus_tag="VM841_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HVL90462.1"
                     /translation="MFPLSKGRITKQAHVGLPDGTYEEEHGREAFEGRASHLYRTHPP
                     TAWVAIEGPLRPRAIDVAAIKPADMSDAGGTWARIAHNEDCSLYVSRRTEPMPYFLRD
                     ADGDLCYFVHRGRGTMETDYGPLTFSPGDYLIVPKGTTHRFVPDEELFLFVIEGAGEY
                     HIPDRGMLGRHAQFDPGVIATPEPDPHDETGRFEVRVKRGNAYTALTYAYHPLDVVGW
                     KGDLCPMRLNVKDFRPIVSPRYHLPPSVHCTWANQGFAVCTFAPRPTETGDPDALRVP
                     FFHSNIDNDEVIFYHDGQFFSRAGITPGMMTIHPQGLHHGPQAPALEAAKTKEFADEI
                     AVMVEAVKPLTVDEDALAAVIDGYETSWAKGYGFE"
     gene            8223..8603
                     /locus_tag="VM841_09560"
     CDS             8223..8603
                     /locus_tag="VM841_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90463.1"
                     /translation="MRSRILRAAVLASLLLGLVPALGSDSVLAGHSGGGAQIGGFEID
                     GDFSYPHDPPIAGATTDWATAPTPVSRGDDAEGSSIDDVFGGGAKEQSPDTWTFDDGP
                     KPPGKDDLTRFYGSASVTASDALP"
BASE COUNT         1453 a         2860 c         3007 g         1286 t
ORIGIN      
        1 gatcgacgcc gatcgagacc cgcatcggca cggctcccgg actcatcgtc gagcatggcg
       61 gcggcgtcgt ctacgcgatc cccggcgtcc cggccgagat gcgcgagatg atgagccgcg
      121 cggtcctgcc cgacctcgcc cgccgcgccg gcacgaccgc cgtcaccagg acgcgggtgg
      181 tgcgcgtgtc gggcatggcc gagtcctcga tcgccgaagc gctgtcgccg ctgtggcgct
      241 ccctcgacga cgcgccggtg aagatgtcgt atctcgccgg cgggggagag gtgcgtgtgc
      301 gcatcaccgc gaaggcactc gatgccgcgg cggccgacgc cctgctggat ccggtggagc
      361 gatccattcg cgaaacgctc ggcacggccg tggtcggaac cggggacgcg acgctggaat
      421 cggtcgtcgg agaccttctc aaagaagtcc gggcgacgct cgccgtcgcg gagtcggtca
      481 ccggaggcgg tgtcgccgag cgcatcacgc gcatccccgg atcctccgct tacttcgccg
      541 gcggcgtcgt ggcctacgac cccgccgtca agacggccct gctcggcgtg gatccggcgc
      601 tgatcgaaat ggacggcgtc gtctcggaac gcgtcgcgct cgcgatggcg cgcggcgtgc
      661 gcgagcgcac cggcgcgacc gtggggctcg cgctcaccgg cgctgccggg ccggacgcgc
      721 acggcggcat gccgcccggc agcgtgtgga tcgcggtgga cgacgcgtcg gcgtcggcgg
      781 cgcgtctggt gcgctacgcg agcgaccggg cggccgtgcg cgggttcgcg accactgccg
      841 cgttgaacct gctgcgtctg catctgtcgg aggcccggcg atagcgcgcc tgttcgtcgc
      901 ggtcgggctc tccgatgagg cgagagccga ggccgcacgg gccatcgacg atcttcgccc
      961 ggtcgtggcc gccgcccggt gggtcccgcc cgagaatctt cacgccaccg tcgcgttcat
     1021 cggcgagacc ccggacgcgg acgcggtgat cgaggcggtc acccgcgcgg cgtcgccgtt
     1081 tcccccggtc cggctggtgc tcgaagggct cggagccttc ccgtcgtggc gccgggccag
     1141 ggtcttgtgg gcgggcctga ccggcgaggt ggcggtcctt tcggccgcgg cccgggcggt
     1201 cggggaagcc ctcgcgccga tcggatacga gcccgaggac cggccgtaca cggcccacgt
     1261 cacgctggcg cgcctgcgca gcccggggcc ggtcgccgcg gcgtcggccg cggtcggacc
     1321 cgtttcgttc ctcgcggagg gactgggcgt ctaccgatcc cttttgggcc gcccggccgc
     1381 ccgatacgag cggctggccc tggccccgtt ctccgcctcg cgggcgtgac cggcgagcgc
     1441 cgttccgctc gttctgcccg cacgggattg cgccggcgcc gcgggctacc gtggcctccg
     1501 gcgtcgttga cgtcgaacag gtgttcgcct aaagtcgccg gcgaacgaca cgcctgtgat
     1561 tttgtcggcg ggccggtcta gcatcggttc gacgaaacga gacgaacaga aaagacgggc
     1621 agggaaggga gagccagatg gcggaacagg acaaggccct cgaggccgcg ctgggacaga
     1681 tcgagaagca gttcggcaag ggctcgatca tgcgcatggg cgagaacgag aagatgcgca
     1741 tcggagtcat cccgaccgga tcgatcgcga tcgaccaggc cctgggaatc ggcggcatgc
     1801 ctcgcggccg catcgtcgag atctacggtg cggagtccag cggcaagacc acgctcgccc
     1861 tgcacgtcgt ggcggaggcg cagcgcgccg gcggcatcgc ggcctacatc gacgcggagc
     1921 acgccttgga tcccgtctac acggccaatc tcggcgtgaa tctcgacgag ctcctgatct
     1981 ctcagcccga caccggcgag cagggcctcg agatcgccga cacgctcatc cggtccggtg
     2041 cggtggacgt cgtggtgatc gactcggtcg cggcgctcgt gccccgggcg gagatcgaag
     2101 gtgagatggg ggacactcac gtcggccttc aggcgcggct gatgtcccag gcgctgcgca
     2161 agctgtcggg caacctgagc cgctcgaaca cgtgcgcgat cttcatcaac caacttcgcg
     2221 agaagatcgg cgtgatgttc ggctcgcccg aaaccacgcc cggcggccgg gccctgaagt
     2281 tctactcgtc ggtccgcgtg gacgtgcgcc gaatcgagaa cctcaaggac ggagccgagg
     2341 tcgtcggttc gcgagtgcgc gccaagatcg tcaagaacaa gaccgctccg ccgttccgtc
     2401 aggccgagtt cgacatcatc tacggcaagg gcatctcgaa ggaaggctcg ctgatcgacg
     2461 tcggtgtgga cctgggcttg gtcaagaagt cgggcgcgtg gttcacctac gacggtacgc
     2521 agatcggcca gggccgggag aacgcccgcc agttcctgct ggacaacgtc gacgtcgcct
     2581 acgacctgga gaagcgcatt cgcgatgcgc tgggcctcgt gccgcaggag ggcggcgagc
     2641 cggtcgtggt gcccgtcgag gcgccggccg aggacgagaa gtagtccggc ggcggctcac
     2701 gctcctgggg aaggcggcat gagcacgaag ccgggcgacg gggaggcatc agcctccccg
     2761 tcctcttcct tccgcggttc ccgcaggcgt gcgggaacct ggccctccga ggatgcctcc
     2821 accccgcagc agcggcgccg gaacgccacc gaggtggtcc tgcggatgct caccgcgcgc
     2881 gagcgatccg aggccgaggt ccggcgggct ctgtccaagc gcggatatga cccggatacc
     2941 atcgccgtgg tggtccagga gttcatatcc aagaaccttc tcagcgacgc ccggttcgcc
     3001 gaggcgttcg ccgaggaagc ggcgcagcgg cgcggttacg gcgcggcggc catccggttc
     3061 aagctccgcg gcaagggggt ctcgcccgaa ctggccgccc gggccgccgc tgtctcgccc
     3121 gaggacgagg aggagcgggc ccgcgagctg gccgccaaga aggtgcgggc catgaccgcc
     3181 ctctcacccg atgtgcgcta ccgaaggctc gccggagcct tggcccgccg gggatatccc
     3241 gccgacatcg tctcgcgcgt cgcggctgac ttatcccggc acgagcctcg ggcgcccgga
     3301 gacgaatagc cacgccggac acccggtctc cttgagtggg aaaacctgcc ggtgctagga
     3361 tcggatttgc tcggttgtga atgcgattcc cgatgacgaa ttcgaccatc tgacgaaccg
     3421 gaccggcccg cgaatgacga tgacgaggac agaaacctcc taggacctgc gaaaacgcag
     3481 tgttccttcg tcgcgagccc gaaaagggac tcgtgcggcg ctccccggcg ggaccgcatc
     3541 ccccaacgcg agcatgccgt gaagcgcggc gccctcctgt cggggagcgc cgacgacgaa
     3601 ccggccgggc tcgcagttcc ggccgcgatg aggagagtga aacgacatgg ccctcaccat
     3661 cggagtcctg atcgggctcg tgatcggggg cgccatcggg tacttcatcc gtcacagagc
     3721 cggactggcg accggatccg cggccgaggc caaggcacgc agcgtgctgg aggaggccga
     3781 gaaagaggcc gagcgggccc gccgcgacgc cgtggtcgcc gccaaagacg aggcccttcg
     3841 cattcgggag gaggccgact ccgagatcaa agggcgccgg gccgagctgg atcgccggga
     3901 atcccgattc gagcaacgcg agagccacct gcaggctcgc gaggaggatc tggaccgcca
     3961 ggcgatctcg ttggccgacc ggggccggct gctggaagaa cgcgaggagg cgctccgccg
     4021 ggagcgagaa gaactcgagg ggcggagccg ggagctcacc gcccgagccg gcgatctgga
     4081 gcaccagatc gagcgggtcg ccgcgatgac ctcggccgag gccaagagcg tcctgaccaa
     4141 acgaatcgaa gacgatgcca agcgcgaagc gatggcgact gtgcgagagc tcgagacgcg
     4201 ggcgcgcgaa gaggccgaag cgcgggcccg ccggatcgtc acgctggcga tccagcgcct
     4261 ggccagcgaa cagaccgccg agtccacggt ggcggtcgtg tcgctcccga gcgacgacat
     4321 gaaaggccgg atcatcgggc gcgagggccg caacatccgc gcgttcgaat ccgtgaccgg
     4381 cgtgaacatc atcatcgacg acacgcccga ggcggtgttg ctctcgtgct tcgatcccgt
     4441 ccggcgcgag gtcggccgga tcacactcga aaagctcgtc gccgacggcc gcattcatcc
     4501 ctcgcgaatc gaggagatct tcgatcgcgc atcgggcgag gtcgaagagg agatccgcaa
     4561 ggccggcgag gcagcggttc tggacgtcgg gatcaccgac atgcatccgg atctgatcag
     4621 ggtgctgggc cggttgcagt accggacctc gtacgggcag aacgtgctca agcatctgat
     4681 cgagacggcg cacatcgccg gcgtgatggc ggccgagctg gggctccctc ccgaccaggt
     4741 tcgcatggtg aagcgttgcg gcctgctcca cgacatcggc aaggccgtca cccacgaggt
     4801 cgaaggatcg catgcgctga tcggggccga gctggcacgg cgcttgaagg agccggagcc
     4861 ggtggtccat gcgatcgagt cgcaccacgg cgaggtcgag cagcgcacgc tggaggccgt
     4921 cctcgcgcag accgccgatc agatctccgg gggccggccg ggtgcccggc gcgagacgct
     4981 cgagaactac gtcaagcggc tcgagaagct cgaggagatc tgcacgtcgt atccgggcgt
     5041 cgagaagacc tttgccatgc aggcgggccg ggaggttcgc gtgatggtca agcccgaatc
     5101 tctcgacgat ctggcctgtc aggttctcgc gcgcgacatc gcgaagcgca tcgaggaaga
     5161 gctgcaatat cccggacaga tcaagatcac cgtggttcgg gagacacgct cgaccgagtt
     5221 cgcgaaatag gagctcgatt caaggccggc cggagggctc ttcggccggc cttcagctgc
     5281 cccccaattg cgggatatcg agccggggaa aaaatattcg gacaacggtg gacaatacga
     5341 cgtaggcaat ggaaactata gcgggacgtc tgaccaaaac aggacgtcgg aacagagagg
     5401 tccattcctg tggaactgct caaggtctcg gcaaaatcga gccccaattc cgtcgccggt
     5461 gcgatcgcgg gcatcctccg cgagcgaggc caggtggacg tgcaggtcat cggcgccgga
     5521 gcgctcaacc aggccgtcaa ggcgatcgcg atcgcgcggt ctttcgtggc ggaggaggga
     5581 atcgacccgg tctgcattcc cgccttcaag gacatcgaca tcggcgggga aggccgcacg
     5641 gcgatctgcc tgaccgtgct cgatcgcaac cgcgcgtccg ccgcagcccc cgctgccgcg
     5701 acgccggtcg ccgcggcgca gcagcactaa gcggccgccg cccgtcgtcg cctttcggcg
     5761 gcgcatgccg gtgatacgct tcgaatcacg cccaaaaggc agcaacggag gtgacccgtc
     5821 atgagcgacg acgcgtttcc cgttctcggg atcgatcacg tcgaattttg gaccggcaac
     5881 gccaagcagt cggcctactt ctacacacgc gcaatggggt tccgggagat cgccttctcc
     5941 gggctcgaaa cgggcgttcg cgaccggacc acccgcgtcc ttcagcaagg gaagatccgc
     6001 ttcgcgatca gcggcgcgct cggcccctcg tcgccggtcg ccgcgcacgt ggcccggcac
     6061 ggggacgggg tgcgcgacgt cgcgttgcgg gtgcccgacg ccgaggaggc gtaccgggtg
     6121 gcgctcgagc gcggcgccat cgacgtgcat ccgccgcagg tcttcgaaga cgagcacggc
     6181 aaggtcgtgc gcgccgccat cggcacctac ggcgagacca tccacagcct gattcagcgc
     6241 gacgattatt ccggcccgtt cctgcccggc ttcgcggccg tcgccgacga ccggtccgaa
     6301 ggcgggctca agatcatcga tcacatcgtg ggcaacgtcg agatcggcac gatgaacgtc
     6361 tgggccgact actacgcgca ggtgatgggt ttccagaacc tcgtgcactt ccgtgacgac
     6421 cagatctcga ccgagtacac ggcgctgatg tcgaaggtga tgtgggacgg atcagggcgg
     6481 gtgaagtttc cgatcaacga gccagcggaa ggcaagaagc gctcgcagat cgacgagtat
     6541 ctggacttct acgccggccc gggggtgcag catctcgccc tgctcaccaa cgacatcgtg
     6601 gcgacggtga cggagcttcg cgatcgcggc gtcgagtttc tgcgcgtccc ggacgcctac
     6661 tatgaggacg tcgccgagcg gttctcggat ctgagcgagg tggacgtcga ggcattgaag
     6721 cggctggcga ttctcgcgga ccgcgacgaa gaagggtact tgctgcagat cttcagcaag
     6781 ccggtcatgg accgtccgac cgtgttcttc gaggtcatcg agcggcacgg gtcgcgcggg
     6841 ttcgggctcg gcaacttcaa ggcgctgttc gaagcgatcg agcgcgagca ggcgcttcgc
     6901 ggaacgcttt agggggtcgc atgttcccgc tctccaaagg acgcatcacc aagcaggcgc
     6961 acgtcggtct tccggacgga acctatgagg aagagcacgg gcgcgaggcc ttcgagggcc
     7021 gcgccagcca cctgtaccga acccacccgc cgacggcctg ggtcgcgatc gaagggccgc
     7081 tgcgcccgcg cgccatcgac gtcgccgcga tcaagccggc cgacatgagc gatgccggag
     7141 gaacctgggc gcggatcgcc cacaacgagg actgctcgct ctacgtgtcc cggcgcaccg
     7201 aaccgatgcc gtacttcctg cgcgacgcgg acggggacct gtgctatttc gtgcatcgcg
     7261 gacgaggcac gatggagacc gactacggcc cgctgacgtt ttcgcccggt gactacctga
     7321 tcgtgccgaa ggggacgacg catcgcttcg tgcccgacga ggagctgttc ctgttcgtga
     7381 tcgaaggcgc cggcgagtac cacatcccgg atcgcggcat gctgggacgg cacgcccagt
     7441 tcgaccccgg cgtcatcgcc acgccggagc ccgacccgca cgacgagacc ggccggttcg
     7501 aggttcgggt caagcgcggc aacgcctaca ccgcgctgac ctacgcgtac catccgctcg
     7561 acgtcgtggg ctggaagggc gacttgtgtc cgatgcggct caacgtcaag gacttccgtc
     7621 cgatcgtgtc tccgcgctac cacctgccgc cgagcgtgca ttgcacctgg gccaaccagg
     7681 gattcgccgt gtgcaccttt gcgcctcgcc cgaccgagac cggcgacccc gacgcgctgc
     7741 gcgtgccgtt ctttcactcc aacatcgaca acgacgaggt gatcttctac cacgacggcc
     7801 agttcttctc gcgcgcagga atcacgcccg gcatgatgac gatccacccg cagggactgc
     7861 atcacggtcc gcaggcgccc gcgctcgagg cggccaagac gaaggagttt gcggacgaga
     7921 tcgccgtgat ggtcgaggcg gtgaagccgc tcaccgtcga tgaggacgcg ctcgccgcgg
     7981 tcatcgacgg ctacgagacc tcgtgggcga aaggctacgg tttcgagtag cgcattccgt
     8041 ccggttgcgt gaagagtcct gccacggcag gactcttcgt ttttctccgg cgggactagt
     8101 ccttccgtcg ccaggacttt gggccgaact ctctattcct tcggagccat gtccgcgaga
     8161 gcatgacatc gggaacggtt cgtccgcacc atgcggaccc cgtgggagag aggtgacgag
     8221 tcgtgcgttc acgcatcctt cgtgcagcgg tgcttgcaag cctgcttctc ggtctggttc
     8281 cggccctcgg gtcggactcg gtgctcgccg ggcattcggg aggcggcgcc cagatcgggg
     8341 gcttcgagat cgacggggac ttctcgtatc cgcacgaccc gccgatcgcg ggcgcgacga
     8401 cggactgggc gacggcgccg acgcccgtgt cccgcggaga cgatgccgag ggctcgtcaa
     8461 tcgacgacgt gttcggcggc ggcgccaagg agcagagtcc cgacacctgg acgttcgatg
     8521 acggaccgaa gccgccgggc aaggacgacc tcacgcgctt ctacggcagc gcgtccgtca
     8581 ccgcatccga cgcgctgccg tagaag
//
LOCUS       DATLFO010000235        14912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_4713, whole genome shotgun sequence.
ACCESSION   DATLFO010000235 DATLFO010000000
VERSION     DATLFO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14912
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4713"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VM841_09565"
     CDS             complement(<1..616)
                     /locus_tag="VM841_09565"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90464.1"
                     /translation="MAKWGNGITGVSGGLSLPANPDRHTGEYAEPSWIDDEFDLSEFA
                     GADELLLLFSYATDAGLAKRGWFIDDIAVTAGQQTLYKSDFEKDYERTRLSPLGLAGW
                     VRVKAGAPNEVDHAYYIEMRDRVGWDFDSKNQSDRGPPTWEPGVSIIYTDEQHGYGNT
                     GVSNPPAQTPVDSVPTPLSDNPNLDDAAFIAAPGRDLFDGCVHED"
     gene            complement(728..2545)
                     /locus_tag="VM841_09570"
     CDS             complement(728..2545)
                     /locus_tag="VM841_09570"
                     /inference="COORDINATES: protein motif:HMM:NF017368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HVL90465.1"
                     /translation="MIRKRIGLLVAVMAILAAPAVPQALSLAAPPEGISVEASYVSST
                     GWVKPGEVYPFKVIVRGGKTAASNVRINVALPPSASFVSSTPAPAIAAPRAASFTIPT
                     VAPNTTTRIVIQARAASLTEDPEIIWKDLSATATMMIGPQEAGTSVTHGPRVTTLESA
                     RYGDRPFPILMVEYQDVKHCTGEGEPYAECTGNHTAEKFEQAVNSKTSGESVWHLFQD
                     ISLGQLHPKGTVPSAGKGTVPFEGVGGRKWSTPAPSGTCMGVTLAGADGTPAYPNRIE
                     DGWYVLPGQQAYYGSDKVGHGLGRSLTGVEALGGIDAGCGPTGKLVYDSALAADPDLD
                     YNDFDTDRDGVVDFTMVIFAGDGGNGSTTPTGLNNIWPHSSDLRSYFTSPDGQSGYIS
                     QDQLRDTLERPLWFTDATKTKTTTKNMGDALKAFVRVGPYNVNPEPSAEKISVIAHEY
                     GHSLGLPDFYSTGGRSTFGSWELMAGDHAQYSTVFARQDLGWVVPREATDGEYKLRES
                     KIDINEIHWKRPDGTPYVLKGPNIHNADVLRVGIPTDPLLDKAPSGKHVWHSGAGNDF
                     DCPLNGGRGLLVRVPEAASVSEGSSMTLSMKSWYEIEWD"
     gene            complement(2699..3148)
                     /locus_tag="VM841_09575"
     CDS             complement(2699..3148)
                     /locus_tag="VM841_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL90466.1"
                     /translation="MIADGWNVLVFPEGTRSKDGWMGEFRMGAARLCLENGIPAVPIA
                     LRGTYQSMPRGRGWPKPGRLPVNVRFGEPVRPEDGELPPDFAARMRAALELLLEEDRT
                     TWYEALKRDATGDLTPATGPKAAAWRRIWDAGEPVADPVHARRAWPR"
     gene            complement(3145..3579)
                     /locus_tag="VM841_09580"
     CDS             complement(3145..3579)
                     /locus_tag="VM841_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HVL90467.1"
                     /translation="MSGIVKDLRTLQRGFRWGRRPLAPRNVPLPDLAPRDFPTAWART
                     PAAKAARAAIHEAVMKPLIESQIRPRVHNAHVLSTLAPPVIFYANHSSHLDAPTLLLS
                     LPPSWRDRTAVGAAADYFFDVWWRAAATALVINAFPVERPSR"
     gene            complement(3576..5153)
                     /locus_tag="VM841_09585"
     CDS             complement(3576..5153)
                     /locus_tag="VM841_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HVL90468.1"
                     /translation="MSRPGFVLEVDERTPPILFHHGENFRLEKLPLGSRVVYGPDPLP
                     GIGDVDAAIEHALLNPLGMEPLPSLLRPGMRLTIAFDDISLPLPQMRTPDIRGRIIEH
                     VLRHAARAGVDDVRLYVANSLHRRMTAAEIKRAVGERVFRSFWPDALQNHDAEDRDNL
                     AYLGKTEHGEDVEINKHAAESDLLVYVNINLVSMDGGHKSVAVGLGSYRSVKPHHNVE
                     TMLQSKSYMDPRHSALHRSCDRMGKIVEKTVKIFHIETTLNNRAFGAPFEFLQKREWE
                     WTLKDQAAYFAAKRANDRTPVKTRRAMFHKIAAPYGLTGIAAGDVDEVHTVTLERVHR
                     QQIVEVDGQADVLVAGLPYIGPYNVNSIMNPILAVCEGLGYFFNMYRGKPLVRQGGVM
                     IMFHPVRNEFHSLHHPSYIDFFEQVLSVSTDPETIQAKFEQQFAEDEWYRHLYRTGYA
                     YHGVHPFYMWYWASHAMAHLGDVIYVGGDRSTVARLGFRSATTLADALDMASETAGRS
                     PRITAMRTPPLWLAEVR"
     gene            complement(5150..6352)
                     /locus_tag="VM841_09590"
     CDS             complement(5150..6352)
                     /locus_tag="VM841_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVL90469.1"
                     /translation="MTLSLEIYRSVPRYLAARAVGGKLPGMLAGPVAALRLVHRSEPV
                     IDRPGWARITPRLSGVCGSDLTTIAGRSSFYFTALVSMPFVPGHEVVGDLLEACEDLA
                     AGTRVVLDPVLACAARGVEPACEPCARGERGLCERVTGGHLGAGLQTGYCDDTGGGWS
                     QMLTAHRSQLHTIPDGMSDEDAVMVEPLACAIHAVRRANVPRDADVLVAGAGTVGLLT
                     TLALKAETEAARVIVVAKHKHQADLAREFGADEIVSPDQALGSIRRSTGAFRLHPERS
                     GPFLLGGVDVAFDAAGSKSTLDLALRSTKARGTVVLAGMPAKADLTPAWFRELTLTGA
                     YSGAGCFADAIEMAAGNNVGRLVGAAYPLERWREAIDHALEAGRLGTVKVVFKPEGPR
                     ALKSGGVQ"
     gene            complement(6349..8622)
                     /locus_tag="VM841_09595"
     CDS             complement(6349..8622)
                     /locus_tag="VM841_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HVL90470.1"
                     /translation="MIREGLAGRSVALTGVTGFLGQGLLERLVSDIPVERIHVFIRGD
                     APSRLNAITSGAVFGPLRAAIGEDALRERIERLVVAHSADLVADEIAMPADVDTVIHS
                     AATVSFDPPLDEAFDTNLRASLKMLESARGKRYLHVSTAYVAGITRGTQAEEPIRRDV
                     DWRAEAEYALRVRTLAEDASRRPEMLRKLRERARHQVGRAGPQSVAARAEELRREWVR
                     ARLVHHGRERANSLGWQDVYTFTKGLSEIAIAELRGDTPVQIVRPSIIESALRHPYPG
                     WIEGFRVAEPVILAYGRNVIPEFPGIPEGVMDIIPVDFVVNCILAVAAREPEDLAFYH
                     CSTGARNPLSFRGIYENCREYYQAHPLPERGRGTAPVPVWKFPGKRAVSKRMRNAEQA
                     VSLLERGVERLPRGSMARNAARTVDRLRGKLDFVKRYATLYGAYTEVEVIYTDDRARA
                     LYESMPDADRQDFLFDPTVFTWEEYLQQIHNPSITTWLRLPKPPRAEPTVAVQPRSGT
                     APVIAAFDVEGTIVASNVLEAYLWLRMSDAPRDEWAKIIADLTRRLPSLLGAERRDRG
                     EFLRLFYRRYAGAREDELRALAREHMGEMILRRLAPAATRRIREHRAAGHTVVLVTGA
                     ADFVVEPLRPLVDEIVAAELKVRGGRFTGDLVMPPLVGEARASWLRRYAQQIGADLNA
                     SYAYADSMSDLPMLEVAGNPVAVNPDVTLTRVARTRRWPIEEWPAAGGTPKIALPETD
                     ALDAVSVRAALPAGAAR"
     gene            8812..10557
                     /locus_tag="VM841_09600"
     CDS             8812..10557
                     /locus_tag="VM841_09600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HVL90471.1"
                     /translation="MIRVTAVAAVVAVAAIAIPAAAATAPARSSPDREARYLTVFTDG
                     TDRTVVEHHDLRTGQSSVHEIRRDRTRHDEHAQHAAGSGEPAGPSSVRCTNGGTYGYI
                     NWSSGRVPRPWRVVEALRFNPAGAPMTWDWKSQIIEGRTVWEESRNDCGTPDRIAFRF
                     ETGADSTASVAPAPGSSCPPSDGENTMGWTPQPPNVLARACLRSSSLSGYIHEADLLF
                     TTEMPWCNGTTVACTAGRWDLQSVAAHEWGHFLGLGHVCGDPGLSPCDDTSETAAVMH
                     PFIVEGSIQNRTLSAGDINGAASLYPAEYAMNVAGVSLSNPNGGPLIPGETYDVTVDT
                     INTGFEAWPVSPATTLATSPAGRCSAFAAASWSSCTSAASLSQDLTNASHPSIPNDAS
                     VLVNGETGRFTTQVTIPWSAEGTTPAELFVPEIAAPGGPRRPGGTASLPLSVGSFGAE
                     ILSVAGPATLGLADTVARALPSDEVEVRVRNTGTIAWPLGGSMVTLDTALPAGRCSEY
                     AFADWHGCARASHLDDNEDEPGSVLPGQVGVFRFRMMGPFLAEPRESVTEWFALTARD
                     IRTLASPLGITFRSL"
     gene            10599..11189
                     /locus_tag="VM841_09605"
     CDS             10599..11189
                     /locus_tag="VM841_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90472.1"
                     /translation="MAPTLAVLCLLLAGCAAPAEPGSSAHISSSSDALPEHCSPRPNP
                     WPSPFMHRGNATHDDKLAEVGDRVPEFAGLTVDEPRKAAMVRVTDGKDETARTALEAY
                     RDVMGGGGLEGYKAVAVKVSYSWHQLKTWYDTANQAGAWQDGVTLGDIDELENRIHYG
                     ASDPDQVRECIAAVMRHYDIPDDAWHLDRQDPVELL"
     gene            11249..11857
                     /locus_tag="VM841_09610"
     CDS             11249..11857
                     /locus_tag="VM841_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL90473.1"
                     /translation="MNRIDHVIYGTHDVNAAGHRFWDEFGLASYEGGLHPAYGTANRI
                     VPLGDSYIEIMGISDPTAARMNPLGEFVLSQIDDGDRWIAWCLRPGDIEQTAERIGSV
                     AVPGHRVRPDGTSVTWLLAGLEIALSDPPLPFFIAWDDDAHMPGRETLEHRVLADGID
                     GIELVGDATRLREHAGDDVEASVTEGSPPRIVAVTVGGSRIA"
     gene            complement(11854..>14912)
                     /locus_tag="VM841_09615"
     CDS             complement(11854..>14912)
                     /locus_tag="VM841_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90474.1"
                     /translation="QVNRQRIVVALALLLSSLVSAQSTPADQSVTYAGVGEVDATWHV
                     GASAGQYAGDRPCDATVVLRLDPSRVAGSCAGPEAQSTIATFDQGNLSTRRVPSYGIQ
                     SRLSVRALVVDGPDPQDPSVRNRVAIVKNDLYIPQDLLWRRTAQILEERDVQRSLGLL
                     GGVETRIDRNNLTIAVSHNHSSPYYSSPSWGVWAFQDVFDVRFFEYYAQRMARAVEKA
                     AAALVPVRMGAAVTQFDETHRHSFGPAIADDGTPAGYPHSDANHDLMVARFDEIVPGG
                     GTKPLAILMNYALHPEFLDGNNLITGDFIAPLERMVDRETGATLFFTQGDVGTAEPER
                     NVYHSIHDRNDYSHKEYAQAEVGARKMADAVIDTWQDVAAGTPAEPARFVPFQEGFAV
                     KMFDRFFPGPTSHPYPSVSNCRTDRALRGNPQAPLAGLPDCQGPGTSFEGSPFDPGVD
                     TDTFEAAGIPIPENYPALGYTGLHEDLGVHLQAVKLGDLLVTVCSCEQWKDQGKNIQT
                     RTDRVQGNIYLGFEWAKGYSYPGDQTYAPCTQNPDTTWRCPDPRNANATLMLGNVKFR
                     RMQAQIRNDAKGWNDLDSVAWAESEPTDPGRIKGNFTHEELPASGPGSGYAMTMTVAM
                     ANDYNGYLASYREYQRGDHYRKALTGWGPHSQDYFATRLVEAGGWLNGGPAVADEPLD
                     AKETADIAHNEARARILGEIGAKYLTALEAALPADGGFPAIDEQPAARVERFGAAFLS
                     WTGGSNYTDDPFVKVQRFDVESGEWTDFADMSGEVVVTLKFPTLPNDLPAFLTGGLEY
                     RWTATWEVFGSRVADLGGGIGSTPVGTYRFVVDGLRRDASCGSVTGTCPYHLESNSFQ
                     VTRWTGITVPDIRAEPDGRVSFTVGPTTTIPVPRLGGGVPVEAVIGPIDYPDTYDARA
                     EFIRHVRTMYRDPSAPNDPSKFEWYCNECSFRPWLDTGTVSRAIVTVVRADSTIEEVV
                     AVFDSGLGRWVAPAGLQPGDTAYVAPAGITDAGGEINGLPSAVVTR"
BASE COUNT         2274 a         5099 c         5236 g         2303 t
ORIGIN      
        1 tgtcctcgtg cacgcagccg tcgaacaggt cgcgcccggg cgcggcgatg aacgccgcgt
       61 cgtcgaggtt cggattgtcg ctcagcggcg tgggcaccga gtccaccggc gtctgcgccg
      121 gcgggttcga cacgccggta ttgccgtagc cgtgctgctc gtcggtgtag atgatcgaca
      181 cgcccggctc ccaggtgggc gggccgcggt cggactggtt cttgctgtcg aagtcccagc
      241 ccacccggtc gcgcatctcg atgtagtacg cgtggtcgac ctcattgggt gcgccggcct
      301 tgacgcgcac ccagccggcc aggccgagcg gcgacaggcg cgtgcgctcg tagtccttct
      361 cgaagtcgct cttgtagagc gtctgctgac cggcggtgac ggcgatgtcg tcgatgaacc
      421 agccccgctt ggccaggccg gcgtcggtcg cgtacgagaa cagcagcagc agctcgtcgg
      481 cgccggcgaa ctcgctcagg tcgaactcgt cgtcgatcca gctcggctcg gcatattcgc
      541 cggtgtggcg atccgggttg gcgggcagcg acaggccgcc cgagacgccg gtgatgccgt
      601 tgccccactt ggccatgcaa gagttctcgt tggggttgta ggcgttggtg agggtggtgc
      661 ccttccggga ctccagcgac ttccacgtcg cgccgccgtc cccggaagcg agaacaaatc
      721 cgtagtctta gtcccactcg atctcgtacc aggacttcat cgacagcgtc atcgacgagc
      781 cctcggacac cgacgcggct tccggcacgc gaacgagcag gccgcgcccg ccgttgagcg
      841 gacaatcgaa gtcgttgccc gcgcccgagt gccacacgtg cttgccggac ggggccttgt
      901 cgagcagcgg atcggtcggg atgccgacgc gaagcacgtc ggcgttgtgg atgttcgggc
      961 ctttgagcac gtacggcgtg ccgtcgggac gcttccaatg gatctcgttg atgtcgatct
     1021 tggactcgcg cagcttgtat tcgccgtcgg tcgcctcgcg cgggacgacc caacccagat
     1081 cctggcgcgc gaacacggtc gagtattggg cgtggtcgcc ggccatgagc tcccacgagc
     1141 cgaacgtcga acggccaccc gtcgagtaaa agtccggaag acccagcgaa tggccgtact
     1201 cgtgggcgat gaccgagatc ttttcggccg acggctccgg gttgacgttg taggggccga
     1261 cgcgcacgaa cgccttcaac gcatcgccca tgttcttggt cgtggtcttg gtcttggtcg
     1321 cgtcggtgaa ccacagcggc cgctcgagcg tgtcgcgcag ctggtcctgc gagatgtagc
     1381 ccgattggcc gtcgggtgag gtgaagtagg accgcagatc cgacgagtgc ggccagatgt
     1441 tgttgagccc ggtcggcgtg gtcgagccgt ttccgccgtc accggcgaag atcaccatcg
     1501 tgaagtccac gacgccgtcg cggtcggtgt cgaagtcgtt gtagtcaagg tccggatcgg
     1561 cggcgagcgc cgaatcgtag acgagcttgc cggtcggacc gcagcccgca tcgatcccgc
     1621 cgagcgcctc gacgcccgtg agcgagcggc cgagcccgtg gccgaccttg tccgatccgt
     1681 agtaggcctg ctggccggga agcacgtacc agccatcctc gattcggttc gggtacgccg
     1741 gcgtgccgtc ggcgccggcc agcgtcacgc ccatgcaggt gcccgagggc gcgggcgtgg
     1801 accacttccg gccgcccacc ccttcgaacg gcacggtgcc cttgccggcc gacgggactg
     1861 ttcccttggg gtgaagctgg ccgagcgaga tgtcctggaa gaggtgccag acggactcgc
     1921 ccgaggtctt ggagttcacg gcctgctcga acttctcggc cgtgtggttg ccggtgcact
     1981 cggcgtaggg ctcgccctcg cccgtgcagt gcttcacgtc ctggtactcg accatcagga
     2041 tcgggaacgg acggtcgccg tagcgcgcgg actcgagcgt ggtgacgcgc ggaccgtgcg
     2101 tcacggacgt gcctgcctcc tgcggcccga tcatcatcgt cgcggtggcc gacaggtcct
     2161 tccagatgat ctccgggtct tcggtaagcg aggcggcgcg cgcttggatc acgatgcgcg
     2221 tcgtcgtgtt cggcgcgacc gtcgggatcg tgaaggaggc cgcacgcggt gccgcgatgg
     2281 ccggggcggg tgtcgaggag acgaacgacg ccgagggcgg cagcgccacg ttgattcgaa
     2341 cgttggacgc ggcagtcttg ccgccgcgaa cgatcacctt gaatggatag acctcacccg
     2401 gcttcaccca tccggtggac gacacgtagc tcgcctcgac cgagattccc tcgggtggag
     2461 ccgccagcga cagcgcctga ggcacggccg gcgcggccag gatggccatg acggcgacaa
     2521 gcaagccgat ccgtttgcga atcacgactc ctcccgcgca aggttcgaac gatcttcggt
     2581 attcattctt tgcggagcgc tgaattccct cccaccaacc ggacaaaacg tacaattatt
     2641 gcacacgcag ggcgggattt caccgcgatg cgtggcgacc ggggactgcg gcccggcgct
     2701 accgaggcca tgcgcgccgg gcgtgaacgg ggtcggcgac cggttcgccg gcatcccaaa
     2761 tgcgccgcca cgccgccgcc ttcggcccgg tggctggggt cagatcccct gtggcgtcgc
     2821 gcttgagcgc ctcgtaccag gtggtgcggt cctcctcgag cagcagctcg agcgcggcgc
     2881 gcatgcgcgc ggcgaagtcg ggtggcagct cgccgtcctc ggggcggacc ggctcgccga
     2941 agcgaacgtt gaccggcagg cggccgggct tgggccaccc gcggccgcgg ggcatcgact
     3001 ggtaggtgcc ccgcagcgcg atcggcacgg ccgggatgcc gttctccaga cacaggcgag
     3061 cggcgcccat ccggaactcg cccatccagc cgtccttgct gcgcgttccc tcgggaaaca
     3121 ccagcacgtt ccagccgtcg gcgatcatct gcttggccgc tcgaccggga aagcgttgat
     3181 caccagcgca gtcgccgccg cgcgccacca cacgtcgaag aagtagtcgg ccgccgcgcc
     3241 gaccgcggtg cggtcgcgcc acgacggcgg cagcgacagc agcagcgtcg gagcgtccag
     3301 gtgcgaggag tggttggcgt agaagatcac gggcggcgcg agcgtggata gcacatgcgc
     3361 gttgtggacg cgcggccgga tctgggactc gatcaagggc ttcatcacgg cttcgtgaat
     3421 cgcggcgcgc gcggccttgg cggccggagt gcgcgcccac gccgtgggga agtcgcgcgg
     3481 cgcgagatcg ggcagcggca cgttgcgcgg cgcgagcgga cgccgccccc agcggaagcc
     3541 ccgctgcagg gtccgcaggt ccttgacgat gccgctcatc gaacctccgc cagccacagc
     3601 gggggcgtgc gcatcgcggt gatgcgcggc gaccggccgg cggtttcgga cgccatgtcg
     3661 agcgcgtcgg cgagcgtggt ggccgaccgg aaacccagac gcgcgacggt ggatcggtcg
     3721 ccgccgacgt agatcacgtc gcccaggtgc gccatcgcgt gcgaggccca gtaccacatg
     3781 tagaacgggt gcacgccgtg atacgcgtag ccggtgcgat acagatggcg ataccactcg
     3841 tcctcggcga actgctgctc gaacttcgcc tggatcgtct ccgggtcggt gctgaccgac
     3901 agcacctgct cgaagaagtc gatgtaggac gggtggtgca ggctgtggaa ctcgttgcgg
     3961 accgggtgga acatgatcat cacgccgccc tgacgcacca gcggcttgcc gcgatacatg
     4021 ttgaagaagt agccgagccc ttcgcagacc gccaggatcg ggttcatgat cgagttcacg
     4081 ttgtagggcc cgatgtaggg caggcccgcg acgagcacgt cggcttggcc gtccacctcg
     4141 acgatctgct ggcggtgcac gcgctcgagc gtgaccgtgt ggacctcgtc cacgtcgccg
     4201 gccgcgatgc cggtcagccc gtagggcgcg gcgatcttgt ggaacatcgc gcggcgcgtc
     4261 ttgacgggcg tgcggtcgtt ggcgcgcttg gccgcgaagt aggccgcctg gtccttgagt
     4321 gtccattccc actcgcgctt ctgcaggaac tcgaacggcg cgccgaacgc gcgattgttg
     4381 agcgtggtct cgatgtggaa gatcttgacg gtcttctcga cgatcttgcc catccggtcg
     4441 caggagcggt gcagcgccga gtgccgcgga tccatgtagg acttggactg cagcatcgtc
     4501 tcgacgttgt ggtgtggctt gacgctgcgg tagctgccga gcccgaccgc gacggacttg
     4561 tggccgccgt ccatgctcac gaggttgatg ttgacgtaga cgagcaggtc cgactcggcg
     4621 gcgtgcttgt tgatctcgac gtcttccccg tgctcggtct tgcccagata cgcgaggttg
     4681 tcgcggtcct cggcgtcgtg gttctgcagc gcgtccggcc agaacgagcg gaagacgcgc
     4741 tccccgacgg cccgcttgat ctcggcggcg gtcatgcggc ggtgcagcga gttcgcgacg
     4801 tagaggcgaa cgtcgtccac gccggcgcgc gcggcgtggc gcagcacgtg ctcgatgatg
     4861 cgtccgcgga tgtcgggcgt tcgcatctga ggcagcggca gtgagatatc gtcgaaggcg
     4921 atcgtgagcc gcatcccggg acgcagcagc gacggcagcg gctccatacc gagcgggttg
     4981 agcagcgcgt gctcgatcgc cgcgtccacg tccccgatgc ccggcagcgg atcgggcccg
     5041 tagaccaccc gcgatcccaa cggcagcttc tccaaacgga agttctcgcc gtggtggaac
     5101 aagatcggcg gcgtgcgctc gtcgacctcc agcacgaatc ccggacggct cactgcaccc
     5161 ctcctgattt cagggcgcgc gggccctcgg gcttgaatac gaccttcacc gttcccaaac
     5221 ggccggcctc gagcgcatga tcgatcgcct cgcgccagcg ctccagcggg tacgccgcac
     5281 cgaccagacg gcccacgttg ttgccggccg ccatctcgat cgcgtcggcg aagcaccccg
     5341 cgccgctgta ggcgccggtc agcgtgagct cccggaacca ggcgggcgtc aggtcggcct
     5401 tggcgggcat gccggccagc acgaccgtgc cgcgcgcctt cgtggagcgc agcgccaggt
     5461 cgagcgtgct tttggatccg gccgcgtcga aggcgacgtc cacgccgccg agcaggaacg
     5521 ggccggaccg ctcgggatgc agccggaacg cacccgtgct tcggcggatg ctgccgagcg
     5581 cctggtcggg cgagacgatc tcgtcggcgc cgaactcgcg cgcgagatcc gcctgatgct
     5641 tgtgcttggc gaccacgatc acacgcgcgg cctcggtctc ggccttgagg gcgagcgtgg
     5701 tcagcagccc gacggttccc gcgccggcga cgaggacgtc ggcgtcgcgc ggcacgttcg
     5761 cgcgacgcac ggcgtggatc gcgcaggcga gcggctcgac catgaccgcg tcctcgtcgc
     5821 tcatgccgtc ggggatggtg tgcagctgcg agcgatgcgc cgtcagcatc tggctccagc
     5881 cgccgccggt gtcgtcgcag tagccggtct gcagtcccgc gccgaggtgt cccccggtga
     5941 cgcgctcgca caggccacgc tcgccgcgtg cgcacggctc gcatgcaggc tcgacaccgc
     6001 gcgccgcgca cgccagcacc ggatccagca ccacgcgcgt tccggccgca aggtcctcgc
     6061 aagcttcgag caggtcgccg acgacctcgt gtccggggac gaacggcatc gagacgagcg
     6121 cggtgaagta gaaggaggaa cggccggcga tggtcgtcag gtcggatccg cagacgcccg
     6181 acagccgcgg cgtgatccgt gcccagccgg gccgatcgat cacgggctcg cttcgatgca
     6241 ccagccggag cgcggcgacc ggtccggcaa gcatgcccgg aagctttccg ccgacggcgc
     6301 gcgccgccag gtagcgagga acgctgcggt agatctcgag cgacagcgtc atcgtgcggc
     6361 tccggcgggc agcgccgcac gcacggacac agcgtcgagc gcgtcggtct cgggcagcgc
     6421 gatcttgggc gtgccgcccg cggccggcca ttcctcgatc ggccagcggc gcgtgcgcgc
     6481 cacgcgcgtg agcgtcacgt cggggttcac cgcgaccggg ttgccggcca cctcgagcat
     6541 cggcaggtcg ctcatcgagt cggcgtacgc gtaggacgcg ttgaggtcgg caccgatctg
     6601 ttgcgcatac cggcgaagcc acgacgcgcg tgcctcgccg acgagcggcg gcatgaccaa
     6661 gtcgccggtg aagcggccgc cgcgcacttt cagctccgcg gcgacgatct cgtccaccaa
     6721 cggccgcagc ggctcgacga cgaagtcggc ggcgccggtg acgagcacga cggtgtgccc
     6781 ggcggcgcgg tgctcgcgga tgcggcgcgt ggcggcgggc gcgagacggc gaaggatcat
     6841 ctcgcccatg tgctcgcgcg ccagcgcgcg aagctcgtct tcgcgagcgc cggcgtagcg
     6901 gcggtagaac aggcgcaaga actcgccgcg atcgcggcgc tcggccccca gcagcgacgg
     6961 cagccggcgg gtgagatccg cgatgatctt cgcccactcg tcgcgcggcg cgtcgctcat
     7021 gcgaagccac agatacgcct cgagcacgtt ggaggcgacg atggttcctt ccacgtcgaa
     7081 ggcggcgatc accggcgcgg tgccgctgcg cggctggacc gcgaccgtcg gctcggcgcg
     7141 cggcggcttc ggcagccgca accacgtagt gatcgacggg ttgtggatct gctgcaggta
     7201 ctcctcccag gtgaagaccg tcgggtcgaa caggaagtcc tgccggtcgg cgtcgggcat
     7261 cgactcgtag agcgcccggg cgcgatcatc ggtgtagatg acctcgacct cggtgtaggc
     7321 gccgtacaag gttgcgtacc gcttgacgaa gtcgagcttg ccgcgcagac ggtccaccgt
     7381 ccgggcggcg ttgcgggcca tggatccgcg cggcagccgc tcgacgccgc gctcgagcag
     7441 cgacacggcc tgctcggcgt tgcgcatgcg cttggagacc gcgcgcttgc cgggaaactt
     7501 ccagaccggc acgggtgcgg tgccgcggcc gcgctccggc agcggatgcg cctggtagta
     7561 ctcgcggcag ttctcgtaga tgccgcggaa cgacagcggg ttccgggccc cggtcgagca
     7621 gtggtagaag gccaggtcct cgggctcgcg cgccgcgacc gccaggatgc agttcacgac
     7681 gaagtccacc ggaatgatgt ccatgacacc ttcggggatc ccggggaact cgggaatcac
     7741 gttacggccg taggccagga tcaccggttc ggcgacgcgg aacccttcga tccaaccggg
     7801 ataggggtgg cgcagcgccg actcgatgat cgacggacgc acgatctgaa cgggcgtgtc
     7861 gccgcgaagc tcggcgatcg cgatttcgga cagccccttc gtgaacgtat agacgtcctg
     7921 ccacccgagg ctgttggcac gttcgcggcc gtgatggacc agacgagcgc gcacccattc
     7981 gcggcgaagc tcttccgcgc gggccgcgac cgactgcggt ccggcgcgac cgacctgatg
     8041 ccgcgcgcgc tcgcgcagct tgcggagcat ttcgggccgg cgcgaggcgt cctcggcgag
     8101 cgtgcgtacc cgcagcgcgt actcggcctc ggcacgccag tccacgtcgc gccgaatcgg
     8161 ctcctcggcc tgggtgccgc gcgtgatccc ggcgacgtag gcggtggaca cgtgcagata
     8221 gcgtttgccg cgcgcggact cgagcatctt gagcgaggcg cgcaggttgg tgtcgaaagc
     8281 ctcgtcaagg ggcgggtcga acgatacggt cgcggccgag tggatcacgg tgtccacgtc
     8341 ggccggcatg gcgatctcgt cggcgacgag gtcggcgctg tgggcaacca cgagccgttc
     8401 gatccgctcg cgaagggcgt cctcgccgat cgccgcacgc agcggcccga agaccgcgcc
     8461 gcttgtgatc gcgttgagac gggacggcgc gtcgccgcgg atgaagacgt ggatgcgctc
     8521 gacggggatg tcgctgacga gccgctccag caagccttgt ccgaggaagc cggtcacgcc
     8581 ggtcagcgcg accgaccggc cggccagtcc ttctcggatc aacgggcacc ccccaagcgg
     8641 ctctctaccg cgcggtagat tattcctccc gtcccggcac ggcaaaaccg cgaagtccaa
     8701 tccgggaaaa gcgcgaaagg cccggcgagg gcccaaaccg gccggacaat ccggaaggag
     8761 ggcggcagcc ccaccgcgaa ttccgggata ccgacacatg gggggcatca cttgattcga
     8821 gtcacggccg tggcggccgt cgtcgcggtc gcggcgatcg cgatcccggc cgcggccgcc
     8881 acagcaccgg cacggtcctc gccggatcgc gaggcgcggt acctgaccgt cttcaccgac
     8941 ggaaccgatc gcaccgtcgt cgagcaccac gatcttcgaa ccgggcagtc ctcggtgcac
     9001 gagatccgcc gcgaccgcac ccgccacgac gagcacgcgc agcacgcggc cggctccggc
     9061 gagccggcgg gcccgtcgtc ggtccgctgc accaacggcg gcacctacgg atacatcaac
     9121 tggagctccg gccgggtccc gcggccctgg cgcgtggtcg aagcccttcg attcaacccg
     9181 gccggcgcgc cgatgacgtg ggactggaag tcgcagatca tcgaaggccg cacggtgtgg
     9241 gaggagtccc gcaacgactg cgggactccc gaccggatcg catttcgctt cgagacgggc
     9301 gcggactcga cggcctcggt cgcgccggcg cccggctcct cgtgcccgcc gtcggacggc
     9361 gaaaacacga tgggctggac gccgcagccc ccgaacgtcc tcgcccgtgc gtgcctgcga
     9421 tcgagcagct tgagcggata catacacgaa gccgacctgc tgttcacgac cgagatgccg
     9481 tggtgcaacg gaaccaccgt ggcgtgcacc gccgggcgat gggacctgca gtccgtcgcg
     9541 gcccacgagt gggggcattt cctgggcctg ggccacgtgt gcggcgaccc cggcctgtca
     9601 ccgtgcgacg acacgtccga gacggccgcg gtgatgcatc cgttcatcgt tgagggctcg
     9661 attcagaacc ggacgctgtc ggcgggcgac atcaacggag cggcctcgct gtatcccgcc
     9721 gagtatgcga tgaacgtggc cggggtgtcg ctgtcgaatc cgaacggcgg cccgctgatc
     9781 ccgggcgaga cctacgacgt caccgtggat acgatcaaca ccggcttcga agcctggccg
     9841 gtctcgccgg cgacgacgct ggcgaccagc ccggcgggac gatgcagcgc cttcgccgcc
     9901 gcctcatgga gctcgtgcac gtcggcggct tcgctgtcgc aggacctgac gaacgcgtcg
     9961 cacccgagca tccccaacga cgcgtccgtc ctggtgaacg gagagaccgg ccggttcacc
    10021 acccaggtca ccatcccctg gtccgcggaa gggaccacgc cggccgagct gttcgtcccc
    10081 gagatcgccg cccccggcgg gccgcgccgg ccgggcggca cggcttcgct gccgctttcc
    10141 gtgggctcgt tcggcgccga gatcctttcc gtcgccggac cggcgacgct gggcctcgcc
    10201 gatacggtcg cccgtgccct cccctccgac gaggtcgagg tgcgggtgcg caacaccggg
    10261 acgatcgcgt ggccgctggg cgggtcgatg gtgacgctgg acacggccct gccggcggga
    10321 cgctgcagcg agtacgcctt cgcggactgg cacggctgcg ctcgcgcgag ccacctcgac
    10381 gacaacgagg acgagccggg ctcggtcctt cccggccagg tcggcgtctt ccggttccgg
    10441 atgatgggac cgttcctcgc cgagcctcgc gagtccgtca cggagtggtt cgccctgacc
    10501 gcccgcgaca tccgcaccct ggcatcgccg ctcgggatta cgttccggtc gctctaacag
    10561 aaaggaaccc gcacccgttg cacggcgtca aagcaacgat ggcgccaacc cttgccgtgc
    10621 tgtgcctgct gctggcgggc tgcgccgcgc cggccgagcc cgggtcctcg gcgcatatct
    10681 cgtcctcgtc cgacgcgctg ccggagcact gctccccccg gccgaacccg tggccctcgc
    10741 cctttatgca tcgcggcaac gcgacgcacg acgacaagct cgccgaggtc ggcgaccgcg
    10801 ttcccgagtt cgccggcctg accgtggacg agccccgaaa ggcggccatg gtgcgcgtaa
    10861 ccgacggcaa ggacgagacg gcccgcacgg ccctcgaggc ctaccgcgac gtgatgggcg
    10921 ggggcgggct cgaaggctac aaggcggtcg cggtgaaggt ctcgtattcg tggcatcagc
    10981 tcaagacctg gtacgacacc gcgaaccaag ccggcgcgtg gcaggacggc gtcaccctcg
    11041 gcgacatcga cgagctcgaa aaccgcatcc actacggagc gagcgacccc gatcaggtcc
    11101 gcgagtgcat cgccgcggtc atgaggcact acgacatccc cgacgacgcc tggcacctcg
    11161 accggcaaga ccccgtcgag ctcctgtaga cgctcccgga cagcccgcgg tcccgaaccg
    11221 cgggaccgcg gcgcgctagg ctgcctcggt gaaccgcatc gaccacgtca tctacggcac
    11281 ccacgatgtg aacgccgccg gtcaccgatt ctgggacgag ttcgggctcg cgtcctacga
    11341 gggcggcctg catcccgcgt acggcaccgc gaaccggatc gtgccgctcg gggactccta
    11401 catcgagatc atgggcatca gcgatccgac cgccgcgcgc atgaatccgc tcggtgagtt
    11461 cgtgctctcc cagatcgacg acggcgaccg gtggatcgcc tggtgcctgc ggcccggcga
    11521 catcgagcag accgccgagc gcatcggatc ggtcgcggtt cccggccatc gcgtgcggcc
    11581 cgacggcaca tcggtgacgt ggctgctcgc cgggctcgag atcgcgctgt cggatcctcc
    11641 cttgccgttc ttcatcgcct gggacgacga tgcgcacatg ccgggccgcg agacgctcga
    11701 gcaccgcgtt ctcgccgacg gcatcgacgg catcgagttg gtcggcgacg ccacacggct
    11761 gcgcgagcac gcgggcgatg atgtcgaggc ttccgtcacc gagggaagcc cgccgcgcat
    11821 cgtcgcggtg acggtcgggg gatctcgaat cgcctagcgc gtcaccacgg ccgacggcaa
    11881 tccgttgatc tcgccgcccg cgtcggtgat gccggccggc gcgacgtagg cggtgtcgcc
    11941 cggctgcagc ccggcgggcg cgacccaacg gccgaggccg gagtcgaaga ccgcgacgac
    12001 ctcctcgatg gtcgagtcgg cccgcacgac ggtcacaatg gcgcgcgata ccgtgccggt
    12061 gtccagccaa ggccggaacg agcactcgtt gcagtaccac tcgaacttcg acgggtcgtt
    12121 cggcgcggac ggatcgcgat acatcgtgcg gacgtgccgg atgaactccg cacgcgcgtc
    12181 gtaggtgtcg ggatagtcga tcgggccgat caccgcctct acgggcacgc cgccgccgag
    12241 gcgcggcacc gggatcgtgg tggtcggacc gaccgtgaac gatacgcgcc cgtccggctc
    12301 ggcgcggatg tcgggcacgg tgatgccggt ccagcgagtc acctggaacg agttagactc
    12361 caagtggtag gggcaggttc cggtcactga tccgcacgac gcgtcgcgcc gcaggccgtc
    12421 gacgacgaac cggtacgtgc ccaccggcgt gctgccgatg ccgccgccca ggtccgccac
    12481 ccgcgagccg aacacctccc acgtcgccgt ccagcgatac tcgagcccgc cggtcaagaa
    12541 ggccggcagg tcgttcggca gcgtcggaaa cttcagcgtc accacgacct cgccgctcat
    12601 gtcggcgaag tcggtccatt caccggactc gacgtcgaag cgctgcacct tgacgaacgg
    12661 atcgtcggtg tagttcgagc cgcccgtcca cgacaggaac gcagcgccga agcgctcgac
    12721 gcgcgcggcg ggctgctcat cgatcgcggg gaagccgccg tcggcgggaa gcgcggcctc
    12781 gagcgcggtc aggtacttcg cgccgatctc acccaggatc cgcgcgcgcg cctcgttgtg
    12841 agcgatgtcg gcggtctctt tcgcgtccag cggctcatcg gcgacggcgg gtccgccgtt
    12901 gagccatccg cccgcctcca cgagccgcgt cgcgaagtag tcttgcgaat gcggccccca
    12961 gccggtgagc gccttgcggt agtgatcgcc gcgttgatac tcgcgatacg aggccagata
    13021 gccgttgtag tcgttggcca tggcgaccgt catcgtcatc gcgtagccgc tgcccgggcc
    13081 gctcgcggga agctcctcgt gggtgaagtt gcccttgatc ctgccggggt cggtgggctc
    13141 ggactcggcc caggcgacgg agtcgaggtc gttccatccc tttgcgtcgt ttcggatctg
    13201 ggcctgcatg cggcggaact tgacgttgcc gagcatgagc gtcgcgttcg cgttgcgcgg
    13261 gtcggggcag cgccaggtcg tatccgggtt ctgcgtgcac ggcgcgtagg tctgatcccc
    13321 cggatacgaa tagcccttcg cccactcgaa cccgagatag atgtttccct gcacgcgatc
    13381 ggtgcgcgtc tggatgttct tgccctgatc cttccattgc tcgcaggagc acacggtcac
    13441 gagcagatcg ccgagcttga cggcttgcag atgcacgccc aggtcttcat ggaggccggt
    13501 atagccgagc gccggatagt tctcggggat cgggatgccg gcggcctcga acgtgtcggt
    13561 gtccacgccc gggtcgaacg gcgagccctc gaacgaggtg ccgggccctt ggcagtcggg
    13621 cagcccggcc agcggcgcct gtgggttgcc gcgcagcgcg cggtccgtcc ggcagttcga
    13681 cacgctcggg tacgggtgcg aggtcgggcc ggggaagaag cggtcgaaca tcttgacggc
    13741 gaagccctcc tggaacggca cgaagcgcgc gggttcggcg ggcgtgccgg ccgcgacgtc
    13801 ctgccaggtg tcgatcacgg catcggccat cttgcgtgcg cccacctcgg cctgcgcgta
    13861 ctccttgtgc gaatagtcgt tgcggtcgtg gatcgagtgg tagacgttgc gctcgggctc
    13921 agccgttccc acgtcccctt gcgtgaagaa cagcgtcgcg cccgtctcgc ggtctaccat
    13981 gcgctcgagc ggcgcgatga agtcgccggt gatcaggttg ttgccgtcga ggaactcggg
    14041 gtgcagcgcg tagttcatca agatcgccag cggcttggtc ccgcctccgg ggacgatctc
    14101 gtcgaagcgc gcgaccatca ggtcgtggtt ggcgtccgag tgcggatagc ccgcgggggt
    14161 gccgtcgtcg gcgatggccg gcccgaacga gtggcggtgg gtctcgtcga actgtgtcac
    14221 cgcggcgccc atgcgcacgg gcaccagcgc ggccgcggcc ttctcgaccg cgcgcgccat
    14281 gcgctgcgcg tagtactcga agaaccggac gtcgaatacg tcctggaacg cccacacgcc
    14341 ccacgacggc gacgagtagt acggactcga atggttgtgc gagactgcga tggtcaggtt
    14401 gttgcggtcg atgcgagttt cgaccccgcc gagcagcccc agcgaccgct gcacatcgcg
    14461 ctcctccagg atctgcgcgg tccgtcgcca caaaaggtcc tgcgggatgt acagatcgtt
    14521 cttgacgatc gcgacccggt tgcgcaccga cggatcctgc gggtccggcc cgtccacgac
    14581 gagcgcgcgg accgacaagc gcgactggat gccgtaggag ggaacgcgac gggtggacag
    14641 gttgccttgg tcgaacgtgg cgatcgtcga ctgcgcttcg ggacccgcgc agctgccggc
    14701 gacccgcgac gggtccaggc gcagcaccac ggtggcatcg cacggccggt ccccagcgta
    14761 ctgccccgcc gaggcaccca cgtgccaggt ggcgtccacc tcgcccaccc cggcgtaggt
    14821 gacggactga tcggcgggcg tggactgggc cgagacgagc gaggacagca agagcgcaag
    14881 cgcaacgacg atccgctgcc gattcacctg at
//
LOCUS       DATLFO010000236         6230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_49517, whole genome shotgun sequence.
ACCESSION   DATLFO010000236 DATLFO010000000
VERSION     DATLFO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49517"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VM841_09620"
     CDS             <1..372
                     /locus_tag="VM841_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVL90475.1"
                     /translation="LPQAIERRRELASLYDKALEDIAMVSRPPAPTTEGPYFDVYQNY
                     VIQAERRDDLAAFLTESGVETLISLPIPNHKQPALGLDHFHLPVTEEISRTQISLPLT
                     TELEDGQIEYVADTIRRFYGA"
     gene            362..1021
                     /locus_tag="VM841_09625"
     CDS             362..1021
                     /locus_tag="VM841_09625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HVL90476.1"
                     /translation="MARRPIYAAGKRMLDLVVGVLALAVAGPVMAVVAMIVKRDGGPA
                     LYRGRRVGRDGAVFRMLKFRTMVVDAEKAGPSSTADDDPRITRIGKRLRAHKLDELPQ
                     LLNVIGGSMSLVGPRPQVEHDVAMYTAEERALLSVRPGITDYASIRYRNEGEILKGSA
                     DPAEAYNRLIRPGKIALGLEYVRSCSFGTDLKILWLTMLAMVDHDAAIRRLPKVETSD
                     T"
     gene            1018..1791
                     /locus_tag="VM841_09630"
     CDS             1018..1791
                     /locus_tag="VM841_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90477.1"
                     /translation="MSRTLPAYTDITERIGSPMSGSQIAMANTRYRFAEDHSKGLDVL
                     EIGCGTGFGLPYLVRTARTLIGGDIDWDNVSAAGATGVPVIRFDAHRLPFASASADTV
                     LLFESIYYLRDPALALQEVHRVLRPGGEILICLPNVERPGFHPSPFSVSYPTAAQLGG
                     LLRHAGYEASIYGAFPVGDGGPAQRLYLAAATIAVRLRIIPRTLRGRAKLKRVLFGRL
                     PAFAGVDGITGVQPLDPLDPSQPVRRYTNLYAVGRKQSR"
     gene            1812..2588
                     /locus_tag="VM841_09635"
     CDS             1812..2588
                     /locus_tag="VM841_09635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HVL90478.1"
                     /translation="MAAGKPSRPTPGKKAGNLRTKAEQEVGPAIERIFERSSDSVEQR
                     LEAFPKYVRRQHLTRFIALYELFKQVVDVKGSVVECGVWRGFGLMTWAKLSAILEPNN
                     LTRRVYGFDSFSGFPAVHDKDANAINPAVPGDLASDSYDELQALIEQFDQDRFLGHIP
                     KVELIDGDATETIPKFVAENPHLVISLLFLDFDLYEPTRAAIEHLVPRMPRGAILAFD
                     ELDNPIWPGETLALVETLGVGSLRIRRLPFDPYIGYAVIE"
     gene            2585..3397
                     /locus_tag="VM841_09640"
     CDS             2585..3397
                     /locus_tag="VM841_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVL90479.1"
                     /translation="MTTDSRDLARRLRRDVVRMTHLGKASHVGSCLSIADVLAVLYSD
                     VLRVDPSGPADPNRDRLIMSKGHAAAVLYAVLAERGFFDREILDTYYQDGSPLAGHVT
                     ANMPGVDHSTGSLGHGLSVAAGIALGAKRDGQDLRAYAILSDGECDEGSIWEPILFAA
                     HHRLDNLIAIVDYNKIQSLEAVDKTLALEPFAGKWRAFGWHTIEIDGHDHDAIRAALE
                     RLPNEPGKPSCVIAHTVKGKGVSFMEQSVLWHYRPPDAGEYEAALAELGGAP"
     gene            3394..4314
                     /locus_tag="VM841_09645"
     CDS             3394..4314
                     /locus_tag="VM841_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVL90480.1"
                     /translation="MRTAFVKELTRLAEQNDRIFFITGDLGFGVMEDYAERFPAQYLN
                     AGVAEQNMTGVAAGLAHTGRIVFTYSIANFPTIRALEQVRNDVCYHNLDVKIVSVGGG
                     LAYGSLGYSHHAVEDVAIMRSLPNMCVIAPGDPIEAAAATRAAVARPGPVYLRLGKAG
                     EPAVHAGEIDFEIGRAILMRDGRDATIISTGGMLHASAAAAERLHVEGMAVRHLSMHT
                     VKPLDIDAVEAAARETGRIVVVEEHSATGGLFAAVAETLLRAGLAARVEQVSLPPGIV
                     REVGSQDYLRGLYGLTADGIRDAVRRIVCA"
     gene            4324..4719
                     /locus_tag="VM841_09650"
     CDS             4324..4719
                     /locus_tag="VM841_09650"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HVL90481.1"
                     /translation="MPRRVKAWLPAALVAGLIFVLSHQPSFGIVLYGWVSELVSNIVH
                     FIEYAMLAAAIAFGLRTEGMDAEDAGKLAILVAVLFGVTDEIHQRFVPGRSSSPLDLI
                     PDLAGAWTGVRLSAEWKKRRGKRRSGARP"
     gene            complement(4709..5242)
                     /locus_tag="VM841_09655"
     CDS             complement(4709..5242)
                     /locus_tag="VM841_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HVL90482.1"
                     /translation="MSRFGRAAAIALSVLAVSTTAWAQYPPQNFITVTPPTVVRGQLA
                     IATACCFQGSVTWTAFSHPRHVGTSTAGSGGLTSITWTVPLDMELGDHTLTASGSDRR
                     GRPLTLATRFTVVSETVVAGTRFNRGGVVGSTGGSAGTRLQTTGSNTIPWMLIGIGSV
                     LAGGTMLGVSRRRKVKA"
     gene            complement(5329..>6230)
                     /locus_tag="VM841_09660"
     CDS             complement(5329..>6230)
                     /locus_tag="VM841_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90483.1"
                     /translation="GYTFEAATIAKFVMSDVYQEFDQSRSRERKMAMLDAAGLIVDKI
                     KKGVDPVKLASALASSAHRKRIQVWMRDEAPQRAVAALGVSGSFRPAASGTTMIGVVT
                     QNAGANKLDYYLRREIDIQIRLREDGSASAAVKVTLRNSAPASGLTREVMGPANVPDG
                     EVLYRAGRNVSFLNVYLPAAAARAEWSEAGKPVGTEIQAAPDALIVSRFIDVAPGGSA
                     ETTLTFELPAGSWDEGRVRLLVPRQPAAAADALTVRIFPPEGLTAGGSDQFEVSKMMN
                     RDILVETHIGRPWTDRLKQLVWR"
BASE COUNT         1041 a         2080 c         2044 g         1065 t
ORIGIN      
        1 ctgccccagg cgatcgaacg gcgacgggaa ctggcgagcc tctacgacaa ggcgctcgag
       61 gacattgcca tggtcagccg acccccggct cccacgaccg aaggtccgta cttcgacgtc
      121 taccagaact acgtgatcca ggcggagcgt cgcgacgacc tcgctgcgtt tctcacggaa
      181 agcggggtcg agacgctcat ctctcttccg attccgaatc acaaacagcc ggcgctcgga
      241 ctggaccatt tccatcttcc cgtcactgaa gagatcagcc ggacgcagat ctcgcttccg
      301 ctgaccaccg agctcgagga tggccaaatc gaatacgtgg ccgacacgat ccgtcgcttc
      361 tatggcgcgt aggccgatct acgcagccgg caagcggatg ctggatctcg tggtcggtgt
      421 cctcgctctc gccgtggccg ggccggtgat ggccgttgtg gcgatgatcg tcaagcgcga
      481 cggcgggccg gcgctttatc gcgggcgccg agtcggccgc gacggagccg tcttccggat
      541 gctgaagttc cgaaccatgg tggtcgatgc cgaaaaggcc gggccgagca gcacggccga
      601 cgacgatcct cgaatcacgc ggatcggcaa gcgccttcgg gcgcacaagc tcgacgagct
      661 gccccagctc ctgaacgtca tcggcggctc gatgtcgctg gtcgggccgc gacctcaggt
      721 cgagcacgac gtcgccatgt acacggcgga ggaacgagcg ctgctgtccg tccggcctgg
      781 gatcacggac tacgcttcga tcagatatcg caacgaggga gagatcttga aagggagcgc
      841 cgatccggcc gaggcgtata accgcctgat tcggccgggc aagatcgcgc tcggcctcga
      901 gtatgtgagg tcgtgttcct tcggcaccga tctcaagatc ctatggctga cgatgctcgc
      961 catggtcgac cacgacgcgg cgattcggcg gcttcccaag gtggaaacca gcgacacatg
     1021 agtcgcacgc tgccggcgta taccgacatc accgagcgga tcggctcgcc catgtcgggg
     1081 tcgcagatcg cgatggcgaa cacgcgatat cgcttcgcgg aggaccattc gaaagggctc
     1141 gacgtccttg agatcgggtg cggcacgggc ttcggtctcc catatcttgt ccgcactgcc
     1201 cggacgttga tcggcgggga catcgactgg gacaacgtct cggccgcggg cgcgacgggc
     1261 gttccggtga tcaggttcga cgctcatcgg cttccgttcg cctcggcgtc ggcggacacg
     1321 gtgctgctct tcgaaagcat ctactatctg cgcgatccgg ccctggcgct gcaggaggtc
     1381 catcgagtgc tccggccagg cggtgagatt ctcatctgcc tgcccaatgt cgaacggccc
     1441 gggtttcacc cgtcaccgtt cagcgtttcg tatcccaccg ccgcgcaact gggtggcttg
     1501 ctgcggcacg ccggctatga ggcctcgatc tatggggcgt ttcccgtcgg ggacggcggc
     1561 cctgcccagc gcctctacct ggctgccgcg acgatcgcgg tccggcttcg tatcattcct
     1621 aggacactgc gaggacgggc caagctgaag cgtgtgctgt tcggacgcct ccctgccttc
     1681 gccggtgtcg acggcatcac gggcgtgcag cccctggacc cgctcgatcc gtcgcagccg
     1741 gtccgtcgct acacgaacct gtacgccgtg gggcgcaagc aaagtcgctg aacgaagaga
     1801 gggggagctc gatggccgcc gggaagccgt ccaggccgac gccgggcaag aaggccggca
     1861 acctgcgtac caaggccgag caggaagtcg gcccggcgat cgagcgcatc ttcgagcgca
     1921 gcagtgattc ggtcgagcaa cgcctcgaag cgttccccaa gtacgtccgg cgtcagcacc
     1981 tgactcgatt catcgcactg tacgagctgt tcaagcaggt cgtggatgtc aaaggttccg
     2041 tcgtcgaatg cggcgtatgg cgggggttcg gtctgatgac atgggcgaag ctcagcgcga
     2101 tcctcgagcc gaacaacttg acccggcgcg tctacggctt cgattcgttc agcggcttcc
     2161 cggccgtcca cgacaaggac gcgaacgcga tcaacccggc cgtgcccggc gacctggcat
     2221 ccgactccta cgacgagctt caagcgctca ttgagcagtt cgaccaggac cgcttcctgg
     2281 ggcatatccc gaaggttgag ctgatcgacg gtgacgcgac cgagacgatc ccgaagttcg
     2341 ttgccgaaaa cccgcacctc gtgatcagcc tgctgttcct cgacttcgac ctctacgagc
     2401 cgacgcgggc cgcgatcgag catctcgtcc cgcggatgcc tcgcggggcg atccttgcct
     2461 tcgacgagct cgacaacccc atctggcccg gcgaaacact cgcgctcgtc gaaacgctcg
     2521 gcgtcggaag cctccgaatt cgccgccttc ccttcgatcc ctacatcggc tacgcggtga
     2581 tcgaatgacg acggattcac gcgacctcgc gcggcggctt cggcgcgacg tcgttcgcat
     2641 gacccatctc ggcaaggcgt cgcacgtcgg ctcttgcttg tcgatcgcgg acgtgctggc
     2701 cgtgctctac agcgacgttc ttcgcgtgga tccgtccggc ccggccgacc cgaaccggga
     2761 ccggctgatc atgtccaaag gtcacgcggc ggctgtgctg tacgcggtgc tggccgagcg
     2821 aggattcttc gaccgcgaga tcctcgacac ctactatcag gacggctccc cattggccgg
     2881 ccacgtgacg gcgaacatgc cgggggtgga tcactcgacg ggttcgctgg ggcacggctt
     2941 gtcggtcgct gcgggcatcg cgctgggcgc gaagcgagac ggtcaggacc tgcgcgcgta
     3001 cgcgatcctg tctgacggcg aatgcgacga aggctcgatc tgggagccga tcctgttcgc
     3061 cgcccaccac cggctcgaca acctgatcgc gatcgtggac tacaacaaga tccaatcact
     3121 ggaagcggtc gacaagacgc tcgcgctcga gcccttcgcc ggcaagtggc gcgcgttcgg
     3181 ctggcacacg atcgagatcg acggccatga tcacgatgcg atccgcgcgg cccttgagcg
     3241 gctgccgaac gagccgggta agccgtcctg cgtgatcgca cacaccgtca aaggaaaagg
     3301 cgtgtcgttc atggagcagt cggtgctgtg gcactaccgg ccccccgacg ccggcgaata
     3361 cgaagcggcg ctggccgagt tgggaggcgc gccgtgagaa ccgcattcgt caaagagttg
     3421 acgcgcctgg ccgaacagaa cgaccgcatc ttcttcatca ccggcgacct cgggttcggc
     3481 gtcatggaag actacgcgga acgctttccc gcgcagtatc tgaacgcggg cgtcgccgag
     3541 cagaacatga ccggcgtcgc ggccggtctt gcgcacaccg gccggatcgt cttcacctat
     3601 tcgatcgcca acttcccgac gatccgcgcg ctcgagcagg tgcgcaacga cgtctgctac
     3661 cacaacctcg acgtgaagat cgtctcggtc ggcgggggac tggcctacgg atcgctcggc
     3721 tacagccatc acgcggtcga ggacgtggcg atcatgcggt cgctgccgaa catgtgcgtg
     3781 atcgcgcccg gcgatccgat cgaggccgcg gccgcgaccc gtgccgccgt cgcgcggccc
     3841 gggccggtct acctccggct cggcaaggcc ggcgagccgg ccgtgcacgc cggcgagatc
     3901 gacttcgaga tcggccgcgc catcctgatg cgcgacggcc gggacgcgac catcatctcg
     3961 accggcggca tgctccatgc gagcgccgct gcggccgaaa ggctccacgt cgaaggaatg
     4021 gccgtccgtc acctgagcat gcacaccgtc aagccgctcg acatcgacgc cgtcgaagcg
     4081 gccgcgcgag agacgggccg gatcgtcgtc gtcgaggaac actccgcaac cggtggcctg
     4141 ttcgccgcgg ttgcggaaac attgctccgc gcggggctgg ccgcgcgtgt cgagcaggtc
     4201 tcgctcccgc ccgggatcgt gcgcgaggtc gggagccaag actatctccg cggcctctac
     4261 ggcctgaccg ccgacgggat ccgcgacgcc gttcgccgca tcgtgtgcgc atagactcgt
     4321 tgcatgcccc gccgcgtcaa agcttggttg ccggccgcct tggtcgcggg gctgatcttt
     4381 gtcctgtcgc atcagccttc gttcggcatc gtgctctacg gctgggtatc cgagctggtg
     4441 tcgaacatcg tgcacttcat cgagtacgcg atgctcgcgg cggcgatcgc gttcgggctg
     4501 cgcaccgagg gaatggatgc cgaggacgcg ggcaagctcg cgatcctggt ggccgtgctc
     4561 ttcggcgtca ccgacgagat ccatcagcgg ttcgtgcccg gacgctcgtc gagcccgctc
     4621 gacttgattc ccgaccttgc cggggcatgg acgggcgtgc ggctctcggc cgaatggaag
     4681 aagcggcgcg gaaagaggcg gagcggcgct aggccttgac cttgcggcgg cgggatacgc
     4741 cgagcatcgt tccgccggcg agcacgctgc cgatgccgat cagcatccaa gggatggtgt
     4801 tgctgcccgt ggtctgcagc cgggttccgg ccgatcctcc cgtgctcccc acgaccccgc
     4861 cgcggttgaa tcgcgtcccg gcgacgacgg tctcggatac gacggtgaag cgggtggcca
     4921 gcgtgagcgg ccggccgcga cgatcgcttc cggaggcggt cagcgtgtga tccccgagct
     4981 ccatgtcgag cggcaccgtc caggtgatcg aggtcagccc gccggagccg gcggtgctcg
     5041 tccccacgtg ccgcggatgc gagaacgccg tccaggtcac gcttccttgg aagcagcaag
     5101 cagtcgcgat cgcgagctgt ccgcgaacga cggtcggggg ggtcacggtg atgaaattct
     5161 gcggcggata ctgtgcccat gcggtcgtcg agacggcgag aaccgacagc gcgatggcgg
     5221 ccgctcgacc gaaacgactc atgcttgcgg gacccccaaa gagaagatgc ttatcagact
     5281 cgaaaatatc cgttacgacc ataaatgtct actgtggtgt ttcttaattc accgccaaac
     5341 aagctgtttc aggcgatcgg tccaaggccg cccgatatgc gtttccacca gaatgtcccg
     5401 attcatcatc ttcgagactt cgaattggtc gctgccgccg gccgtcaggc cttcgggcgg
     5461 aaagatccga accgtgagcg cgtcggccgc cgctgccggc tgacgcggca cgagcagacg
     5521 gacgcggccc tcgtcccacg agccggcggg tagctcgaag gtcagcgtcg tctcggccga
     5581 gccgcccggc gccacgtcga tgaagcgcga cacgatcagc gcgtcggggg cggcttggat
     5641 ctcggtgccg acgggcttgc cggcctcgct ccactcggcg cgcgccgccg cggccggcag
     5701 atagacgttg agaaacgaca cgttgcgtcc cgcgcgatac agcacctcgc cgtcgggcac
     5761 gttcgccgga cccatcacct cgcgcgtcaa tccggatgcg ggcgcgctgt ttcgcagcgt
     5821 caccttgacc gccgcgctcg cgctgccgtc ctcgcgcagc cggatctgga tgtcgatctc
     5881 gcgccgcagg tagtagtcga gcttgttcgc gccggcgttt tgtgtcacca cgccgatcat
     5941 cgtcgtgccg gacgcggcgg gccggaacga gcccgagacc ccgagcgccg cgacggcgcg
     6001 ctggggggcc tcgtcgcgca tccagacctg gatgcgcttg cgatgcgccg aagacgcgag
     6061 cgccgaggcg agcttgaccg gatccacgcc cttcttgatc ttgtccacga tcagcccggc
     6121 cgcgtcgagc atcgccatct ttcgctcgcg cgagcggctc tgatcgaact cttgatacac
     6181 gtcgctcatg acgaacttgg cgatggtggc ggcctcgaag gtgtatccgg
//
LOCUS       DATLFO010000237         4257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_108252, whole genome shotgun sequence.
ACCESSION   DATLFO010000237 DATLFO010000000
VERSION     DATLFO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108252"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="VM841_09665"
     CDS             complement(<1..1683)
                     /locus_tag="VM841_09665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL90484.1"
                     /translation="MRTIRFRLATSLLIAVALTAGAWIGLGLGIFNGQQLRLADALFA
                     GGQPIDSRIVVVAIDDQSLEKRGRWPWDRTVHAALIDELTAAGVQRVGYDILFTETTS
                     PASDEALRASLARAGNVVLASAGTFGRRPGDVLKTTAYQRPVEALAGAALAVAHVNVF
                     PDKDGVVRSLPVVLETGDAELVPALSAALIEPAASTAPITIRPDGIVAGTRFIRTGAA
                     HLLEVNYADKDAFEIVSAADVMDGVVPRDRLNGKIVLVGVTAVGLGDQKLTPIDKSDG
                     LAGVLVHANAANTIATGTYLVPESRVETLFAVFVLALLTSLAVAFLRIWLSPPAAVAL
                     GAAFTWITFMRFDDSRVGNLVYPPITIVLAYIAALAVRYLTEFRERNRVTQVFARYVA
                     KDVVDEVLASPEGAVATLAGASRPMSVLFADLRGFTSASENAKPEEVVAALNVYLDAM
                     TQAVVDEQGTVDKFMGDCVMAFWGAPRPEPQHAERAARAAVRMLDNIDRAIKERPETA
                     LLRVKGCGVGIATGDVVVGNIGSHQRLDYTVIGDTVNTASRLCGVADAGTVII"
     gene            complement(1688..>4257)
                     /locus_tag="VM841_09670"
     CDS             complement(1688..>4257)
                     /locus_tag="VM841_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90485.1"
                     /translation="IVAFEGAICRRVAGQQITFAAQGFDQFGQSVGFVTSSTDFTISP
                     DGACSGNVCVATIAGAHRVTATNGSATDLALFNVIPGPATRLQIAPSNATISPGDSQA
                     YSATASDQFGNSLGDITGTTSFDITPDGDCTGAVCTATVAGPHTVTGTRGTLSDTASL
                     NVMVGTVHSIAITPSSSSIPAGGSQTYFTEGFDEEGNSLGDVTGLTTFTIAPAGGEVT
                     LASSPCAGNVCSATVAGPHTVTAASGEAIVTASLLVEPGEAATVVIDPASAAVPAGTP
                     QSFTVVAFDGHGNEIGDVTEDTFFSIEQAGVTAMEAAGASCDGSTCTAYGAGEWLVRA
                     AYGDTDAIATITVEAGPLASIELAPPEPTIQAGDTQEFTAFGYDEHGNPIEYVTEDTT
                     FTIEPAEFAVEAEPTYGCADNVCGASQAGFFIVTGTIGEIWDTSYLTVEAGEPEYVVI
                     SSDASETTAGQPVEFWLEAFDGYGNSLDDVTEDANFTISRFDEIEAETTGCDDNACSA
                     TTAGTYIVTGEWNGLSDTTSLTVVEGPIDSLVLTPATSTVAPGQPQLYEATASDEYGN
                     SWDATAATNFAIATGTCTNNSCSSSTLGTHTVTGTISGVSGTASLTIESPEGKLTISP
                     ANNTIDLGQSQTYTASFKPKDGTAFDVTDATIFEFSGDDAGKGSCTGNICTPNTTGKN
                     LVIHGRYLDKQDNAKLTVNEPKKTSTAAVTTADAPAPVSPGDNTPAAPGTPAWTQPRL
                     SPQVQQPAAVAPVMPVAPRQVEPVAPPAATLPQSVLRPSPRPQTLPAVTVPAEPQRRT
                     PAPAPEETPVTPSRSSGRTPPVLPEDETDVEATAPTAPPAGRRGRATRNEDDETGGI"
BASE COUNT          625 a         1431 c         1509 g          692 t
ORIGIN      
        1 gatgatcacc gtgccggcgt cggccacgcc gcacagccgg ctcgccgtat tcacggtgtc
       61 gccgatcacg gtgtagtcca atcgctggtg cgagccgatg ttgccgacga cgacgtctcc
      121 ggtcgcgatc cccacgccgc agcccttgac ccgaagcagc gcggtctcgg gccgctcctt
      181 gatcgcccgg tcgatgttgt cgagcatgcg aacggccgcg cgggcggcgc gctcggcatg
      241 ctgcggctcc ggccgcggtg caccccagaa ggccatcacg cagtcgccca tgaacttgtc
      301 cacggttcct tgctcgtcga cgaccgcctg cgtcatcgcg tccaagtaca cgttgagcgc
      361 ggcgacgacc tcctccggct tcgcgttctc cgacgcagag gtgaagccgc gaagatcggc
      421 gaacagcacc gacatcgggc gcgacgcgcc ggccaaggtc gcgaccgcgc cttccggcga
      481 ggccaagacc tcgtccacga catcctttgc cacgtagcgg gcgaagacct gcgtgacgcg
      541 gttgcgctca cggaactcgg tcaggtaacg caccgccagc gccgcgatgt acgccagcac
      601 gatcgtgatc ggcggataaa ccagatttcc tacccgcgag tcgtcgaagc gcatgaacgt
      661 gatccaggtg aaggccgcgc ccagcgcgac cgccgccggc ggcgacagcc agatccgcag
      721 gaacgcgact gcgagcgagg tgagcaacgc caggacgaag accgcgaaga gcgtctcgac
      781 ccgggactcc ggcaccagat aggtgcccgt cgcgatggtg ttggccgcgt tcgcgtgcac
      841 gagaaccccg gcgagcccat cgctcttgtc gattggggtg agcttctggt cgcccagacc
      901 caccgcggtc acgccgacga gaacgatctt gccgttgagc ctgtctcgcg gcacgacgcc
      961 gtccatcacg tcggctgccg agacgatctc gaaggcgtcc ttgtcggcgt agttcacctc
     1021 gagcaggtga gccgcgccgg tacgaatgaa gcgcgtgccg gcgacgatgc cgtccggacg
     1081 gatcgtgatc ggcgccgtgg acgccgccgg ctcgatcaac gcggccgaca aggcgggcac
     1141 gagctcggcg tcgccggtct cgagcacgac cggcagcgag cgcaccacgc cgtccttgtc
     1201 ggggaagacg ttgacgtgcg cgacggccag cgcggcaccg gcgagggctt cgacgggccg
     1261 ctgatacgcg gtcgtcttca gcacgtcacc cggacgccga ccgaacgtcc cggcggaggc
     1321 gagcacgacg tttccggccc gggcgagaga ggcccgcagc gcctcatccg acgcgggcga
     1381 ggtcgtttcg gtgaacaaga tgtcgtagcc gacccgctgc acgccggcgg cggtgagctc
     1441 gtcgatcagg gccgcgtgga ctgtgcggtc ccaaggccag cggccgcgct tctccaggct
     1501 ctgatcgtcg atcgccacga ccacgatgcg agagtcgatg ggttgcccgc ccgcgaagag
     1561 cgcatccgcc agccgcagct gctggccgtt gaagatgccc aggcccagcc cgatccatgc
     1621 tcctgcggtg agcgcgaccg cgatcagcag cgaggtcgcg agacggaacc gaatggtgcg
     1681 cattgcgcta gatgccgccg gtctcgtcgt cctcgttgcg cgtcgcgcgc ccacgccggc
     1741 cggccggcgg cgcggtggga gcggtcgctt cgacgtcggt ctcgtcctcc ggcaggaccg
     1801 gtggcgtgcg gccgctgctg cggctcggcg tcaccggagt ctcttccggg gcgggcgccg
     1861 gcgtccggcg ctgcggctcg gccgggacgg tcacggccgg aagcgtctga ggcctcgggc
     1921 tgggacgcaa cacgctctgc ggcagggtcg cggccggcgg cgcgaccggc tcgacctggc
     1981 gcggggccac cggcatgacc ggggcgaccg cggcgggctg ctgaacctgc ggcgagagcc
     2041 tcggctgggt ccacgccggc gtcccgggcg ccgcaggcgt gttgtcgccc ggcgaaaccg
     2101 gagcgggcgc atcggccgtc gtgaccgcgg ccgtcgacgt cttcttcggc tcgttcacgg
     2161 tcagcttcgc gttgtcctgc ttgtcgaggt agcgaccgtg gatgacgagg ttctttccgg
     2221 tcgtgttcgg agtgcagatg ttgcccgtgc acgacccctt gccggcatca tcgccggaga
     2281 actcgaagat cgtggcgtcg gtcacgtcga acgccgttcc gtccttcggc ttgaacgatg
     2341 ccgtatacgt ctgcgattgc ccgaggtcga tggtgttgtt cgcgggcgag atggtcagct
     2401 ttccttccgg cgactcgatg gtgaggctgg cggtgcccga gactccgctg atcgttccgg
     2461 tcaccgtgtg cgtcccgagg gtggaggaag agcacgagtt gttcgtgcac gtccccgtcg
     2521 cgatggcgaa gtttgtcgcg gccgtcgcgt cccaggaatt gccgtactcg tcggaggcgg
     2581 tcgcctcgta gagctggggc tgacccgggg cgacggtgct ggtcgccggg gtcaggacca
     2641 aggaatcgat cggtccttcc acgacggtta gcgacgtcgt gtcgctcaag ccgttccatt
     2701 cgcccgtgac gatgtaggtg ccggccgtgg tggccgagca ggcgttgtcg tcgcagccgg
     2761 tcgtctcggc ctcgatctcg tcgaaccgag agatcgtgaa gttcgcatcc tcggtcacgt
     2821 cgtcgagcga gttgccgtag ccgtcgaagg cctcgagcca gaactcgacg ggctgccccg
     2881 cggtggtctc cgaggcgtcc gaggagatga cgacgtactc gggctcgccg gcctcgacgg
     2941 tgagatagga cgtgtcccag atctcaccga tcgtgccggt cacgatgaag aagccggcct
     3001 gtgacgcgcc gcacacgttg tcggcgcagc cgtaggtggg ttcggcctcg accgcgaact
     3061 cggcaggctc gatggtgaac gtcgtgtcct cggtgacgta ctcgatcggg ttgccgtgct
     3121 cgtcgtagcc gaacgccgtg aactcctggg tgtcaccggc ctgaatcgtc ggctccggcg
     3181 gggcgagctc gatcgatgca agcggaccgg cctccacggt gatggtcgcg atcgcgtcgg
     3241 tgtcgccgta ggcggcgcga acgagccatt cgccggcccc gtaggcggtg caggtcgaac
     3301 cgtcgcagga cgccccggcc gcttccatcg cggtcacgcc ggcctgctcg atcgagaaga
     3361 acgtgtcttc cgtgacgtcg ccgatctcgt tgccgtgtcc gtcgaaggcc accacggtga
     3421 acgattgcgg cgttcccgcc gggaccgccg cgctggccgg gtcgatgacg accgtggcgg
     3481 cctctcccgg ctcgacgagc aggctcgcgg tgacgatcgc ctcgccggag gccgccgtca
     3541 ccgtgtgagg gcccgccacg gtcgcgctgc acacgttccc cgcgcagggc gacgacgcca
     3601 acgtgacctc gccgccggcg ggcgcgatcg tgaacgtcgt cagcccggtc acgtcgccga
     3661 gggagttccc ttcctcgtcg aagccttcag tgaagtatgt ctgcgatccg ccggccggga
     3721 tcgacgagga cgacggcgtg atcgcgatgg agtgcacggt tccgaccatg acgttcaggg
     3781 acgcggtgtc gctcaaggtc cctcgggttc cggtgaccgt gtgagggccg gcgaccgtcg
     3841 ccgtgcagac ggcgccggtg cagtccccgt ccggggtgat atcgaaggac gtggtgccgg
     3901 tgatgtcacc cagcgagttg ccgaactgat cggatgccgt cgccgagtac gcctggctgt
     3961 cgccgggcga gatcgtcgcg ttcgaaggcg cgatctgcag gcgcgtggcc gggcccggga
     4021 tcacgttgaa cagcgccagg tccgtggcgg agccgttcgt cgccgtcacg cgatgggcgc
     4081 cggcgatcgt cgcgacgcag acgttgcccg aacaggctcc gtccggcgag atcgtgaagt
     4141 ccgtgctcga agtgacgaag cctacggatt gaccgaactg gtcgaaccct tgcgccgcga
     4201 acgtgatctg ctgcccggcc acgcgcctgc agatcgcgcc ttcgaacgcg acgatct
//
LOCUS       DATLFO010000238        10904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_143115, whole genome shotgun sequence.
ACCESSION   DATLFO010000238 DATLFO010000000
VERSION     DATLFO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143115"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..972)
                     /locus_tag="VM841_09675"
     CDS             complement(40..972)
                     /locus_tag="VM841_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL90486.1"
                     /translation="MIIGTTVAAAVFFAGCALAFVTLESVVVERARVNRNLRRLKNID
                     VKATELRRKELAKPFATRVAWPVLRLVGRFAKRFTPAGVVDRLSKDLTYAGSPAGWDA
                     ERILAIKLVASGGGFLVPSLGGPLLGAAPIRGVVAGVLFAILGYYAPEWALRSKAGAR
                     QAAIQRALPDALDLLSITVEAGLGFDAAVKRVATQARGPLGDELHRVLQEMQIGKPRA
                     QALRSLADRTSVPELQSFVLAMVQADSFGIAIGQVLHVQAREMRLKRRQRAEERAQKI
                     PVKILMPLLGCVFPSLFVVILGPAAIQIYRSLLS"
     gene            complement(985..1962)
                     /locus_tag="VM841_09680"
     CDS             complement(985..1962)
                     /locus_tag="VM841_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL90487.1"
                     /translation="MTPLLSASLISFAALAIVFGIVFTGRSDREAVKKIERRIKLYSI
                     GARGKRTRTKGVLADSPVARRAVDLVDKLPRNPKRDKALAAQLERAGWPLRPAEMIAI
                     RLAAAAGAGLALAVLLGNPIAGPIAAGVAYLIPKAALDRAVAKRTNAFLMQLPDTLQL
                     LAGSLKAGYGLVQAVDTLVKETPAPTSTEFSRVLAETRLGMPLEESLTQMAERIGSED
                     FRWVVMAIAIQRQVGGNLADLLTTVAATLRDREAVRRQVKVLSAEGRLSAYILIGLPF
                     GIAGYVAMVNPEFLESLTGERVGQIMIAASLVLMGIGAAWMRKIIKIEV"
     gene            complement(1959..3338)
                     /locus_tag="VM841_09685"
     CDS             complement(1959..3338)
                     /locus_tag="VM841_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HVL90488.1"
                     /translation="MSLYERLSKARDVAPAGGGGGSVAELRIADPFAELKRGIHQALV
                     AALGPKLYDKTMTAEQLEAHVRERLGDVLAGTETPLSQADRQRLSREIADDILGHGPI
                     EPYLQDPSVTEVMVNGPYTVYIEREGKIVRTPAQFLDEAHLRHTIDKIVGRVGRRVDE
                     ASPYVDARLPDGSRVNAAIPPIMLDGPVLTIRKFSRDPFKAGDLVSFGTMSAKLAAFL
                     EACVKGKINIIISGGTGAGKTTTLNVVSSFIPEDERIITIEDSAELRLNQPHVVRAES
                     RPPNIEGRGQVSIRDLVRNSLRMRPDRIVVGEVRGGEALDMLQAMNTGHDGSISTAHA
                     NTPRDALSRLETMCLMAGMDLPVRAIREQIASAVQLIVHQSRLKDGTRRITHVTEVVG
                     MEGDVITLQDLFLFDFGMGLDDAGKFRGHVKPTGIRPKFAEHLKDLGVPLPADMFEPE
                     PFAREEAWA"
     gene            complement(3335..4528)
                     /locus_tag="VM841_09690"
     CDS             complement(3335..4528)
                     /locus_tag="VM841_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HVL90489.1"
                     /translation="MIALIDSDPNFQARVSNELGPRTDPIQVLGLPDAEALASGEPGL
                     PDVLILGPSVGAPDALAVAERLRAAAPEVSVVLVTDALTSDLLQAALRAGVRDVLGSS
                     FTAEQLRDAIERAEQVSHGVRDRGGAHHEDHDPSTPCRVVTVFSAKGGVGKSFIAANL
                     AVLLAQRHARVALIDLDLQFGDQAIMMQLFPGRTMSEAAANIERIDAESLGGYLTPHK
                     SGLQLLAAPLEPGLAETIQPAATQRILRLARQSFDVVVIDTPALFTEHVLAALDESDE
                     CIFMTSLDVPSIKNTKLALQTLEMLGVGKDRIRLLLNRADAQVGLRVQEVERMLGARV
                     ATQIPSSREVPLSINQGTPLAFSDPRSGVTAALRELADSVGRGTPVHAPDAKGGRFRL
                     KRKVS"
     gene            complement(4534..5292)
                     /gene="cpaB"
                     /locus_tag="VM841_09695"
     CDS             complement(4534..5292)
                     /gene="cpaB"
                     /locus_tag="VM841_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVL90490.1"
                     /translation="MSIGTVSRRGVSLIVALVLAAFATAAWVSYVRSIEKRAIDGNAT
                     VQVYVAGDVIPAGTTAETADASKLLVKTGVPKRLVAEGAVTELPQLRGTVASVTIQKG
                     EQILRSRFTSPEQAARLLQIPPGLHAMSVEVSAPPGVGGFVQPHDRVSVIAKVQRENT
                     DPLVRFLLQDVAVLAVGSRTSVAQPASGSREAARESAEPSSTDRMLLTLAVSPYQAEQ
                     LAFAVMEGQVYFTLLPPGSEAIGTPGRTAGNVFG"
     gene            complement(5330..6499)
                     /locus_tag="VM841_09700"
     CDS             complement(5330..6499)
                     /locus_tag="VM841_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HVL90491.1"
                     /translation="MMRRDQRGATAVVVAVSLSAVLGAAVLSIDSGSVWRTRRNLVTD
                     TDAAALAAARSLDAQGAKACDATATANARSEAAGLIAANDPDSVMEGFTVKPTGGNCS
                     AESGQVRVDGRLVAQLSFAGVFGIGSVDVASSSIAQWGPLAAATGLRPIGICDKSPHF
                     QEWADHLAGNDAGWDTPPQTHPNYPGGAVVHRIHLQRGSSGCGSASGNWDWLDFNGNH
                     EPNGNSALREWFYNGFPDQVSLGDAATGRVKDCNPNETGAQDGCDPKTGAGGGSFIDS
                     LEYLRDSKITFPIMVYDKVVDRRDPAGCSAPPPWNGSGSNARFCHVAFLLVRIHGWDQ
                     ITGNLGDNSYLDLEFVDEWWVGSIGQEPSGGRPTVHGVALCGGGYGTKIDNTCDV"
     gene            complement(6496..6927)
                     /locus_tag="VM841_09705"
     CDS             complement(6496..6927)
                     /locus_tag="VM841_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HVL90492.1"
                     /translation="MLNRRRREERGASAVEFALVMTVLFMVLFGVVQFGMAYNRAQGL
                     EAASREGARAASVGATYDEILARVRAAQSMFRPADVQITTTPATNGTQRPCSIAGVGN
                     SVRVEAFVPQMSAYAIAIPLWGKRQIRYTATGVFRCERGAP"
     gene            complement(6921..7103)
                     /locus_tag="VM841_09710"
     CDS             complement(6921..7103)
                     /locus_tag="VM841_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL90493.1"
                     /translation="MGKTRRMRRRDQRGATAVEYSLVAALVAAVVIVSVALLGTSSNE
                     LYRCTGDSMASQVTKC"
     gene            complement(7236..7403)
                     /locus_tag="VM841_09715"
     CDS             complement(7236..7403)
                     /locus_tag="VM841_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVL90494.1"
                     /translation="MNVRSERGATAVEYGLMVALIAAVIIAAVALLGSNTSDTFQCTA
                     DSVAARADNCP"
     gene            complement(7620..8279)
                     /locus_tag="VM841_09720"
     CDS             complement(7620..8279)
                     /locus_tag="VM841_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90495.1"
                     /translation="MSGAAVETETLRVVLIDDHTLVRQSVGLMVGAAGGFRVVAEAGG
                     ADAGIAAVSLHRPDLVVLDIGLPGRSGLEVAAALKEAQPGIRIMFLTMHEDDATIASA
                     VGLGADAYVLKSASTDELLMALRAVATGGSFLSPSVARSVMHRSHTRPGALELTSREL
                     EIVRLLADGARPAQIAASLTLSLRTVRNHLANVYAKLGVQSAAQAVAEAYRRGLVARS
                     G"
     gene            complement(8276..9280)
                     /locus_tag="VM841_09725"
     CDS             complement(8276..9280)
                     /locus_tag="VM841_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVL90496.1"
                     /translation="MSNRIMSAARLRPYRRQASPPAVRSHRYAAATARIAMAGVMSLA
                     IATTGGTASPLVPVGVLVAAVLGLQAGIISGAVGGAVIVAASVPALAAETGEGLQALN
                     QAATWLLLFPLAGTATGLVHALRTRPDASRERRRLAADLHDGVAQTLAHLRLELDMLS
                     HPELGRVSDPERLARLARVADRTLTDVRALINDLAAPVPAGGLVAALREHVAGLEGAS
                     GPVVTLDAPASVRTPEGVDGQLLRIAQEALSNAMRHSGGTAVHVRLAAAGGGTVRLSV
                     HDDGRGIAAEATTGGGLGLDTMRERARAIGATIEIGPRPGGGTRVEVTVPAADLTRSA
                     "
     gene            9470..10276
                     /locus_tag="VM841_09730"
     CDS             9470..10276
                     /locus_tag="VM841_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL90497.1"
                     /translation="MGSPLYADPDGPLRLAVIADSTAFTGPAGPLLPDEPGLYPNVAA
                     RAVEDAIGRRVAVTVVAHPGTGVRGTYYDVTKNRHVMFEVLMRADAVILGLGSFDHAP
                     AGVPPVLEAMVQHVRPRSLRKRYRALLRAVHPLGVRLTLGRFTRVPAGEFERLLDRIL
                     HEVRSLTWGAPGVMLGPTSHRSGYYGNVHPQHRARVDLQRRLAERHRYAFVESWPLVE
                     PWIEKLNVDGIHWPFETHEAVGAAIAGALVPQITGDAPKPGIPGYEDTGL"
     gene            10317..>10904
                     /locus_tag="VM841_09735"
     CDS             10317..>10904
                     /locus_tag="VM841_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVL90498.1"
                     /translation="MTDPAAEVVDLLQGLIRNACVNDGTIESGQETKSIDLIESVIAG
                     DGIDIERFEPVAGRANLVARLSGSDPSAPSLALMAHADVVPVTPDRWRHDPFGGELID
                     GEVWGRGAVDMLNLTASMAIAFKRLARRGFRPRGDLLYIAVADEETGGEAGAKWLVEH
                     EPDAIRTDFLLTEFGGMRVPTAPPGAPAFPVAVAEK"
BASE COUNT         1597 a         3823 c         3836 g         1648 t
ORIGIN      
        1 cgagcgaggc atcggtggtg aacgcgagga ttctcatggc tagctcaaca ggcttcggta
       61 gatctggatg gccgcgggcc ccagaatgac gacgaacagc gacgggaaga cgcatcccag
      121 cagcggcatc aggattttca ccgggatctt ctgcgcgcgt tcctcggcgc gctgccgccg
      181 cttcaggcgc atctcgcggg cctgcacgtg cagcacctgg ccgatggcga tgccgaacga
      241 gtcggcctgc accatcgcga gcacgaacga ctgcagctcg ggcaccgagg tgcgatcggc
      301 cagtgaccgc agtgcctggg cgcgcggctt gccgatctgc atctcctgca gcacgcggtg
      361 cagctcgtcg ccgagcgggc cgcgcgcctg tgtcgcgacg cgcttgaccg ccgcgtcgaa
      421 tccgagcccc gcctcgaccg tgatcgacag caggtcgagc gcgtcgggca gcgcccgctg
      481 gatcgcggcc tgccgcgcgc cggccttcga gcgcagtgcc cactcgggcg cgtagtagcc
      541 gaggatcgca aacagcacgc ccgcgacgac gccgcgaatc ggagccgcgc cgagcagcgg
      601 tccgcccagc gacgggacca ggaacccgcc gcccgaggcg accagcttga tggccaggat
      661 ccgctcggcg tcccagcccg ccgggcttcc cgcgtacgtc aggtccttcg agagccggtc
      721 caccacgccc gcgggcgtga atcgcttcgc gaagcggccg accaagcgca gcaccggcca
      781 cgcgacccgc gtcgcgaacg gcttggcgag ctccttgcgc cgcagctcgg tcgccttcac
      841 gtcgatgttc ttcaggcgcc gcaggttccg gttcacacgc gcccgttcga ccacgacgct
      901 ctccagcgtc acgaacgcga gcgcgcagcc ggcgaagaag acggcggcgg caacggtcgt
      961 tccgatgatc atcgcctctc ctccctagac ttcgatcttg atgatcttgc gcatccaggc
     1021 ggctccgatt cccatcagca ccagggatgc ggcgatcatg atctggccga cgcgttcgcc
     1081 ggtcagcgat tcgaggaact ccgggttcac catcgcgacg tagccggcga tgccgaacgg
     1141 caacccgatc aggatgtagg ccgacaggcg gccttccgcc gacagcacct tcacttggcg
     1201 gcgcaccgcc tcgcggtcgc gcagcgtcgc cgcgaccgtg gtgagcaggt ccgcgaggtt
     1261 gccgccgacc tgacgctgaa tggcgatcgc catcacgacc cagcggaagt cctcgctgcc
     1321 gatccgttcg gccatctgcg tgagcgactc ctcgagcggc atgcccaggc gcgtctcggc
     1381 cagcacgcgc gagaactcgg tcgaggtcgg ggccggcgtc tccttcacca gcgtgtccac
     1441 cgcctgcacg agcccgtagc cggccttgag cgatcccgcc agaagctgga gcgtgtcggg
     1501 aagctgcatc aggaacgcgt tggtgcgctt ggcgacggcc cggtccagcg ccgccttcgg
     1561 gatcaggtac gcgacgcccg ccgcgatcgg cccggcgatc gggttgccca gcaaaaccgc
     1621 gagcgcgagc cccgccccgg ccgccgctgc cagacggatc gcgatcatct cggcgggacg
     1681 cagcggccat ccggcgcgtt cgagctgcgc ggcgagcgcc ttgtcccgct tcgggtttcg
     1741 tggcagcttg tccaccagat cgacggcgcg tcgcgcgacc ggcgaatcgg cgagcacgcc
     1801 tttggtgcgg gtgcgcttgc cccgggcgcc gatcgagtac agcttgatgc ggcgctcgat
     1861 cttcttgacg gcctcgcggt cgctgcggcc ggtgaagacg atgccgaaca cgatcgccag
     1921 cgccgcgaac gagatcagcg atgcggaaag aagcggcgtc atgcccacgc ctcctctcgc
     1981 gcgaacggct cgggctcgaa catgtcggca ggcaacggca cgccgaggtc cttgagatgc
     2041 tcggcgaact tcggccggat cccggtcggc ttgacgtggc cgcggaactt gccggcgtcg
     2101 tcgagcccca taccgaagtc gaacaggaac aagtcctgca gcgtgatcac gtcgccttcc
     2161 atcccgacca cctcggtaac gtgcgtgatc cgccgggtcc catccttgag gcgcgactgg
     2221 tgcacgatca gctggaccgc cgacgcgatc tgctcgcgga tcgcgcgaac cggcaggtcc
     2281 attccggcca tcaggcacat cgtctcgagg cgcgacagcg cgtcgcgcgg cgtgttggcg
     2341 tgcgcggtcg agatcgatcc gtcgtggccg gtgttcatcg cctgcagcat gtccagcgcc
     2401 tcgccgccgc ggacctcgcc gaccacgatc cggtcgggtc gcatgcgaag ggagttgcgg
     2461 accaggtcgc ggatcgagac ctgcccgcgg ccttcgatgt tcggcggccg cgactcggcg
     2521 cgcacgacgt gcggctggtt caggcggagc tcggcggagt cttcgatcgt gatgatgcgt
     2581 tcgtcctcgg ggatgaacga cgatacgacg ttgagcgtgg tcgtctttcc ggcgccggtg
     2641 ccgcccgaga tgatgatgtt gatcttgccc ttgacgcatg cctccaggaa cgcggccagc
     2701 ttcgcgctca tcgttccgaa cgagacgagg tcgccggcct tgaacgggtc gcggctgaac
     2761 tttcggatgg tcagcacggg accgtccagc atgatcggcg gaatggcggc gttgacgcgg
     2821 ctgccgtcgg gcaggcgggc gtcgacgtag ggggaggctt cgtcgacgcg ccgcccgacc
     2881 cgtccgacga tcttgtcgat cgtgtggcga aggtgcgcct cgtcgagaaa ctgcgccggc
     2941 gtgcgcacga tctttccttc gcgctcgata tagacggtgt aggggccgtt gaccatgacc
     3001 tcggtgacgg acgggtcctg caggtagggc tcgatcggtc cgtgcccgag gatgtcgtcg
     3061 gcgatctcgc gcgacaggcg ctgccggtcc gcctgggaca gcggcgtctc ggtcccggcc
     3121 aggacgtcgc cgagccgctc gcgcacgtgc gcctcgagct gctcggccgt catcgtcttg
     3181 tcgtagagct tggggcccag cgcggcgacg agcgcctgat ggatgccgcg cttgagctcg
     3241 gcgaacggat cggcgatgcg aagctcggcg acgctgccgc ctccgccgcc cgccggcgcg
     3301 acgtcgcgtg ccttggagag tcgctcgtac aggctcatga caccttcctc ttcaagcgga
     3361 accggccgcc cttggcatcg ggcgcgtgga cgggtgtgcc gcggccgacc gagtcggcga
     3421 gctcgcgcag tgcggcggtg acgccggagc gcggatccga gaaggccagc ggcgttcctt
     3481 ggttgatcga cagcggcacc tcgcgtgacg acgggatctg cgtggccacg cgggcgccga
     3541 gcatgcgctc gacctcttgc acgcggagcc cgacctgcgc gtcggcgcgg ttgagcagca
     3601 gccggatgcg gtccttgccg acgccgagca tctccagcgt ctgcagcgcc agcttcgtgt
     3661 tcttgatgct gggcacgtcc aggctggtca taaagatgca ctcgtccgac tcgtcgagcg
     3721 cggcgagcac gtgctcggtg aacagcgccg gggtgtcgat gaccaccacg tcgaacgact
     3781 ggcgtgccag ccgcaggatc cgctgcgtcg cggccggctg gatcgtctcg gccaggcccg
     3841 gctcgagcgg cgccgccagc agctgcagcc ccgacttgtg cggggtcagg taaccgccga
     3901 gcgactcggc gtcgatccgt tcgatgttcg ccgcggcctc cgacatcgtg cggccgggga
     3961 acagttgcat catgattgcc tggtcgccga actgcaggtc caagtcgatc aaggcgacgc
     4021 gcgcgtggcg ctgcgcgagc agcaccgcaa ggttcgcggc gatgaaggac ttgcccacgc
     4081 cgcccttggc ggagaagacc gtcaccacgc ggcacggcgt ggacggatcg tggtcctcgt
     4141 gatgcgcgcc gcctcggtct cgcacgccgt gcgagacctg ctcggcccgc tcgatcgcgt
     4201 cccgcagctg ttcggccgtg aacgacgagc cgagcacgtc gcgcacgccg gctcgcaacg
     4261 cggcctgcag caggtcgctg gtgagcgcgt cggtgaccag cacgacgctg acctcgggcg
     4321 cggccgcgcg cagccgctcg gcgaccgcga gtgcgtccgg cgctccgacc gacggtccga
     4381 ggatcagcac gtccggcagt cccggctcgc ccgacgccag tgcttcggcg tcgggcaagc
     4441 ccagaacctg tatcggatcg gtccgcgggc ccagctcgtt cgacacgcga gcctggaagt
     4501 tcggatcgga atcgatgagc gcgatcatgc gaatcacccg aacacgtttc cggccgtgcg
     4561 gcccggtgtg ccgatcgcct cggagcccgg cggcagcagg gtgaagtaga cctggccttc
     4621 catcaccgcg aaggcgagct gctcggcctg gtacggcgag accgcgagcg tgagcagcat
     4681 ccggtcggtc gaggacggct cggcgctctc gcgagccgcc tcacgcgagc ccgatgcggg
     4741 ctgagcgacg gaggtgcgcg agccgacggc gagcaccgcc acgtcctgca gcaggaacct
     4801 caccagcgga tcggtgtttt cgcgctgcac cttggcgatc accgagacgc ggtcgtgcgg
     4861 ctgcacgaag ccgccgacgc ccggcggcgc cgacacctcg acgctcatcg cgtgaaggcc
     4921 gggtgggatc tgcagcagcc gcgcggcctg ctcgggcgag gtgaatcgcg agcgaaggat
     4981 ctgctcgccc ttttggatcg tgaccgaggc gacggtgccc cggagttgcg gcagctccgt
     5041 caccgcgcct tcggccacga gtcgcttcgg cacgccggtc ttgaccagca gcttgctcgc
     5101 gtccgccgtc tcggcggtcg tgccggccgg gatcacgtcc ccggcgacgt agacctgcac
     5161 ggtcgcattg ccgtcgatcg cgcgcttctc gatcgagcgc acgtaggaca cccaggccgc
     5221 cgtcgcgaag gcggccagca ccagggcgac gatgaggctc acccctcgcc gcgacaccgt
     5281 tccgatactc atggtcactc cctcccgtga agcggttggt cactcgtcgt cacacgtcgc
     5341 aggtgttgtc gatcttggtt ccgtagccgc cgccgcacag cgcgacgccg tgcacggtcg
     5401 gccggccgcc gctgggttcc tggccgatgg atccgaccca ccactcgtcc acgaactcca
     5461 ggtccagata cgagttgtcg ccgaggttgc cggtgatctg gtcccatccg tggatgcgaa
     5521 ccagcagaaa cgccacgtgg cagaagcgcg cgttcgatcc cgagccgttc cagggcggcg
     5581 gcgcgctgca gccggccggg tcgcggcggt ccacgacctt gtcgtagacc atgatcggga
     5641 acgtgatctt gctgtcgcgc agatactcga gcgaatcgat gaaggacccg ccgccggcgc
     5701 cggtcttcgg gtcgcatccg tcctgcgcac ccgtctcgtt cgggttgcag tccttgacgc
     5761 ggccggttgc ggcgtcgccg agcgagacct ggtccgggaa accgttgtag aaccactcgc
     5821 gcagcgccga gttgccgttc ggctcgtggt tgccgttgaa gtcgagccag tcccagttgc
     5881 cggacgcgct tccgcagccc gacgacccgc gctgcagatg gatgcgatgc acgaccgccc
     5941 cgcccgggta gttcggatgc gtctgcggcg gcgtgtccca gccggcatcg ttgccggcga
     6001 gatggtcggc ccactcctga aagtgcgggc tcttgtcgca gattccgatc ggacggaggc
     6061 cggtcgcggc cgcgagcgga ccccactgcg cgatcgagga cgacgcgacg tcgaccgatc
     6121 cgatgccgaa cacgcccgcg aagctgagct gggcgaccag ccggccgtcc acccgcacct
     6181 ggccggactc ggccgagcag ttgccgccgg tcggcttgac cgtgaatcct tccatcaccg
     6241 agtcgggatc gttggccgcg atcagccccg ccgcctcgga ccgcgcgttc gcggttgccg
     6301 tggcgtcgca tgccttcgcg ccctgtgcgt ccagggagcg cgcggcggcc agcgcagccg
     6361 cgtcggtgtc ggtgaccagg ttgcgtctgg tccgccagac gctgcccgag tcgatggata
     6421 gcacagccgc gccgagcacg gccgacaagg agacggcgac gaccaccgcg gtcgcgcctc
     6481 gttggtcacg tcgcatcatg gtgccccccg ttcgcagcgg aacactccgg tggcggtgta
     6541 gcggatctgg cgtttgcccc acagagggat cgcgatcgcg tacgcgctca tctgcggcac
     6601 gaacgcctcc acgcgaacgc tgttgccgac gcccgcgatg ctgcacggcc gttgcgtgcc
     6661 gttcgtcgcg ggggtcgtgg tgatctgcac gtccgcgggc ctgaacatcg actgggcggc
     6721 gcggacgcgt gcaaggatct cgtcgtaggt ggcaccgacg gacgcggcgc gcgcaccctc
     6781 gcgggacgcg gcttccaggc cttgcgcgcg gttgtatgcc atgccgaact gcaccacccc
     6841 gaacaggacc atgaacagca cggtcatcac gagcgcgaac tcgacggcgc tcgcgccgcg
     6901 ctcctcgcgc cgccggcggt tcagcatttc gtcacctgcg atgccatgga gtcgccggtg
     6961 caccggtaca gctcgttgga ggatgtgccc agcagggcga cgctcacgat gacgacggcg
     7021 gcgacgaggg ccgcgaccaa gctgtactcg acggcggtgg cgccgcgctg gtcgcgccgg
     7081 cgcatacggc gggttttccc cacgatcctc tcctcccaga ggtgctgctc gactgccgaa
     7141 cccggtgcgg tgacgcgaat ctcggcccgg acgcccggga cgggagtgac ccgtcccgag
     7201 cgacctgcgg ccgttggtgg ggtggtgcgg tgccgttacg ggcagttgtc cgcgcgcgcg
     7261 gcgaccgagt cggccgtgca ctggaacgtg tccgaggtgt tgctcccgag cagcgcgacg
     7321 gcggcgatga tcacggcggc gatcagcgcg accatcaggc cgtactcgac tgcggtcgca
     7381 ccacgctcgc tgcgaacgtt catgcgtgcg acgcagtact tcgcgaaggt gcgggtgaaa
     7441 ccgacgagac ccgacatgcg atacctcctc ctcccgcttc cggcatctcc cctccggagc
     7501 gcctcgaaag cgtgttccca ccgaagagat cggagcagga cggaatcgcg atcagagaca
     7561 ggcgacgtga cttgcgatcg cgtttcgatc taggggagtg agtcgaatga cctaggaggt
     7621 cagccggaac gtgcgaccag cccgcggcgg taggcctccg cgacggcctg ggcggcgctt
     7681 tgcacgccga gcttggcgta gacgttcgcg agatggttgc gcaccgtgcg cagcgacagc
     7741 gtcagcgacg ccgcgatctg cgccggccgg gcgccgtcgg cgagcagccg gacgatctcg
     7801 agctcgcggc tcgtcagctc gagcgcgccg ggacgggtgt gcgagcgatg catgacgctg
     7861 cgcgccaccg aggggcttag gaacgatccg cccgtggcga ccgcgcgaag cgccatcagc
     7921 agctcgtcgg tcgaggccga cttgagcacg taggcgtcgg cgccgagccc gaccgcgctc
     7981 gcgatcgtcg cgtcgtcctc gtgcatggtc aggaacatga tccggattcc cggctgcgct
     8041 tccttcagcg cggccgcgac ctccaggccg cttcgcccgg gcagaccgat gtcgagcacg
     8101 accaggtcgg ggcgatgcag cgagaccgcg gcgatgcccg cgtcggcccc cccggcctcg
     8161 gccacgacgc ggaagccgcc cgcggcgccg accatcagcc cgacgctttg ccggacgagc
     8221 gtgtggtcgt cgatcaagac aacccgaagc gtttcggttt cgaccgccgc gccgctcatg
     8281 cggacctcgt caggtcggcc gccggcacgg tgacctcgac gcgcgtgccg ccacccggcc
     8341 gggggccgat ctcgatcgtc gcgccgatcg cccgtgcacg ctcgcgcatc gtgtcgagcc
     8401 cgaggccgcc gcccgtggtc gcttcggcgg cgatcccgcg tccgtcgtcg tgcaccgaca
     8461 gccggaccgt accgccgccg gcggcggcga gccgcacgtg cactgcggtt ccgcccgagt
     8521 ggcgcatcgc gttcgagagc gcttcctgcg cgatgcgcaa cagctgtccg tccacgccct
     8581 caggtgtgcg gaccgacgcc ggggcgtcga gcgtcaccac cgggccggaa gcgccctcga
     8641 gccctgcgac gtgctcgcgg agcgcggcga ccaggccccc ggccggcacg ggggcggcga
     8701 gatcgttgat cagcgcgcgc acgtcggtga gtgttcggtc cgcgacccgc gccaggcgcg
     8761 cgagccgctc cggatcggag acgcgcccca gctcggggtg ggacagcatg tcgagctcga
     8821 gccgcaagtg cgcgagcgtc tgcgcgacgc cgtcgtgcag atcggctgcg aggcggcggc
     8881 gctcacggga cgcgtcggga cgagtccgca gcgcatgcac caggcccgtc gccgtgccgg
     8941 ccaaggggaa gagcagcagc cacgtcgcgg cctggttgag cgcctgcagc ccttctcccg
     9001 tctcggccgc gagcgccgga accgacgcgg ccacgatcac ggccccgccg accgctcccg
     9061 agatgatgcc cgcctgcagg ccgagcacgg ccgccaccag caccccgacg ggaacgaggg
     9121 gagaggcggt gccgccggtc gtggcgatgg cgagggacat cacgccggcc atggcgatgc
     9181 gcgccgttgc ggccgcgtag cggtgcgacc ggacggcagg cggcgacgcc tgtcgccgat
     9241 acggacgaag gcgcgctgcg gacatgatgc ggttcgacat caaagcgctc ccgagcgtcg
     9301 gtccaccccc acgactggta tctcggcacc cggccggaaa tcgttgacct tcgcgtatgt
     9361 cgcgcccggc ggcgaagcgg gcgaaacggc ggcgcgggag cacacttttt tcctaggcct
     9421 ctaggcgaaa cggcctcgat ggtgcgagcc gggtaccatc ggcggcgaca tgggctctcc
     9481 gctttacgcc gatccggacg gtccgttgcg tcttgcggtg atcgcggact cgaccgcctt
     9541 caccgggccg gcggggccgc tgctgcccga tgagccgggc ttgtacccga acgtggcggc
     9601 acgcgcggtc gaggacgcca tcgggcggcg cgtggccgtc acggtggtcg ctcatcccgg
     9661 caccggcgtg cggggcacgt actacgacgt gaccaagaac cgtcacgtga tgttcgaggt
     9721 gctaatgcgt gccgacgcgg tcatcctcgg cctcggctcc ttcgaccacg cgccggcggg
     9781 ggtgcctccg gtccttgaag cgatggttca gcatgtgcgg ccccggtcgc tgcgcaagcg
     9841 ctaccgggcg ctgctccggg cggtgcatcc gctcggggtt cgcctgacgc tgggacggtt
     9901 cacgcgcgtt ccggccgggg agttcgagcg gctcctcgat cgcatcctgc acgaggttcg
     9961 ctcgctgact tggggcgcgc ccggcgtgat gctggggccg accagccatc ggagcggcta
    10021 ctacggcaac gtgcatccac agcatcgtgc gcgcgtggat ctgcagcgac ggctggcgga
    10081 gcggcaccgg tatgcgttcg tcgaatcctg gccgctggtc gagccgtgga tcgagaagct
    10141 caacgtcgac ggcatccact ggccgttcga gacgcacgag gcggtgggcg cggcgatcgc
    10201 cggcgcgctc gtgccgcaga tcaccggcga cgcgccgaag ccgggcatcc ccggctacga
    10261 ggacaccggc ctgtgacgct tgccgtggcc cggccgcgac ggctatcgtc ccgctcgtga
    10321 ccgatcctgc cgccgaagtc gtcgatctgc tgcaaggctt gatccgcaac gcgtgcgtca
    10381 acgacggcac gatcgaatcc ggtcaagaaa cgaagagcat cgacctgatc gagtccgtga
    10441 tcgccggcga cggcatcgac atcgagcgat tcgagccggt ggccggccgc gcgaatctcg
    10501 tcgcgcgcct gagcggttcc gatccgagcg cgccgtcgct cgcgctgatg gcgcacgccg
    10561 acgtcgtgcc ggtcacgccg gatcgatggc gccacgaccc gttcggcggc gagctgatcg
    10621 acggcgaggt ctggggccgc ggcgcggtgg acatgctcaa cctgaccgcc tcgatggcga
    10681 tcgcgttcaa gcggctcgcg cgacgcggat tccgtccgcg cggcgatctg ctctacatcg
    10741 cggtggccga cgaggagacc gggggcgagg ccggcgcaaa atggctcgtc gagcacgagc
    10801 ccgacgcgat ccgaaccgac ttcctgctga ccgagttcgg cggcatgcgc gtgccgaccg
    10861 caccgcccgg cgcgcccgcg ttccccgtcg ccgtggcgga gaag
//
LOCUS       DATLFO010000239         6774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_64267, whole genome shotgun sequence.
ACCESSION   DATLFO010000239 DATLFO010000000
VERSION     DATLFO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64267"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1669
                     /locus_tag="VM841_09740"
     CDS             170..1669
                     /locus_tag="VM841_09740"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HVL90499.1"
                     /translation="MVLDDLVSTAANVAATSSRNEKIDLLAQALRQTSPGEIEAAVGF
                     LVGWPRQGRIGVGGATAYGLDVAPAPEPTLTVRDVDDAIERVRAITGTGSVQARAEIL
                     TDLLARATSDEQVFIRRIFTGEMRHGALEGVVAAAVAKAAGVPATVVRRATMLSGDLC
                     ATARIALTEGRDGLERIGLTVLRPVLPMLASTAETVQDALTFDGPASVEWKLDGIRIQ
                     AHRDGDEVRVFSRNGNDLTDRLPGVVTVVHGLPVRAVVLDGEAIGLDESERPYAFQDT
                     MSGTGRRTEAFGAVPFFFDVLHLDGEDLLDLPLVERLDALERVAGHRRIRSTVTEDAA
                     EAQRFLDSALAAGHEGIMVKDLSSPYQAGRRGKAWRKVKPAKTFDLVVLAAEWGHGRR
                     RGWLSNLHLGALDDNGTPVMVGKTFKGMTDALLAWQTEKLQSIKEREEGITVWVRPDL
                     VVEIELEGVQTSTRYPGGIALRFARLLRYRDDKTAADADTLESLRALQP"
     gene            1850..2299
                     /locus_tag="VM841_09745"
     CDS             1850..2299
                     /locus_tag="VM841_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90500.1"
                     /translation="MDREGSATGTSAAIVGSAVGAYAGFFVFGAIGFGVASLIMAGAP
                     ECTEKLCGLGQALAAVVWGIVIAAVGAITMMLLGCRYALVKKGFDRVGPTLVWLAFLA
                     PAILIAGAAILPDALSAAWVFATPLMAAPAARRVATKRRGKPAQLAG"
     gene            complement(2444..2848)
                     /locus_tag="VM841_09750"
     CDS             complement(2444..2848)
                     /locus_tag="VM841_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90501.1"
                     /translation="MKGFARTAAALMLAASMAVPAPAGAAVISWCNGSWTNAGACNFV
                     FAGGAFYAGGSATSSTTGARVTVSIVEPVTGTVLHSCAASTSDGRSVSCGAASTRLPS
                     DPQAELTVGAVLRCVVTGMQSGSFLCTSYPVV"
     gene            3061..3630
                     /locus_tag="VM841_09755"
     CDS             3061..3630
                     /locus_tag="VM841_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90502.1"
                     /translation="MADITDEDGEVAEEAIRWESDLNGFLGTGEDVAISGLKEGTHTI
                     RVTADDGRGGMAADEITVRIVRDPTALPATPSRVTASPSMLQCDPSEQLMQHDVVVED
                     ENYMLPAKVFRATASEPWVKLMPVPRGQDVRVSAPYGAMSVAGLTPFSLRVTCEGVGN
                     VSQGIHSAVVTIDLVPSGRLTVPVTLRTT"
     gene            complement(3725..5815)
                     /locus_tag="VM841_09760"
     CDS             complement(3725..5815)
                     /locus_tag="VM841_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90503.1"
                     /translation="MSRRLQLGWIFAAVAALASLPAGAQVGADLAPTGHAVAADSALI
                     ASPVSSTAAWTGTPTMIAGTSRYDAGEWIYTDFVYDDYGADTTPAGQPNIVSLASTSG
                     DFRYPRTPAYAGNAADVVEVRTRVAPNGSDLEVRVLLQTIVDPDVVALWVSANDVETI
                     VTTDNAVVNAAANTVTFTLAGAAADDAVVLNIGAGLNNGSGELRAPVPGSANATPSEF
                     TSGAPTNARLFDLAFNTHAIESRGGAWNEDVQSDAIHAGDLGPFVQTIDVTALKAAAT
                     TPVPDVRTYHVRLFESRQQLGANEGIGTAFPQYLGRFQPYAIWVPESYVPGTPVPLFL
                     NMHSLSVHHNQYRGGAAPSATYRSLYEQYEAKTGAIIVTPLGRGPDGWYEDEALVDTL
                     EVWKDALDLFTIDRDRVYIGGYSMGGYGTYRLGTLIPDAFASAAAVVAPPANGIWAFP
                     LPPTGGESNPDHTFPQLENTRHIPYWLTNGMLDELVPVSGVTRQAMRLGELGHTYRYA
                     LHPAADHFAFAFADEWTREASWLAAHPTRAQNPDRVTLKVRPASWVTSTKPNAAALLA
                     DVNALVSEVGARLDGAYWVGNVQVASGADVTGIVDLRSGGVIMQQTGVSPVATVGADG
                     PSPYLLTGQDRTVAAVAATATLSGSLSKVTSVTVDVGRAGLSDTPVIDVTSDTPAVIT
                     LVRNGVVVGTVAVG"
     gene            complement(5946..6134)
                     /locus_tag="VM841_09765"
     CDS             complement(5946..6134)
                     /locus_tag="VM841_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90504.1"
                     /translation="MRPIDATRLGAHLGLGIHVPDEKAAGRLGAGTFDQARWLVRNVP
                     IKSVGEAMIRMVVQHPYR"
BASE COUNT         1106 a         2248 c         2298 g         1122 t
ORIGIN      
        1 aattctatcg gcgtgaggcg tccgaggcgt cgttggcggc ggcggcggtg gtaggttcgt
       61 tcgatccaga cgacgatcgc gaggcgcagc tcttcgcgcg tctgccaccg gcgcccgatg
      121 cgtggaggcg atcgggagcg acacatcatc gccgggtaga gtttgcccga tggtactcga
      181 cgacttggtt tcaacggcgg cgaatgtcgc ggcaacgtcg tcgcgcaacg aaaagatcga
      241 tctgttggcc caggcgctgc gtcagacatc gcccggcgaa atcgaagcag cagtcgggtt
      301 cctcgtcggc tggccgcggc agggtcgcat cggcgtcgga ggggcaaccg cgtacggcct
      361 cgacgtcgcg ccggcaccgg aacccacctt gacggtacgc gacgtcgacg acgccatcga
      421 gcgcgtgcgg gcgatcaccg ggaccggctc ggtccaggca cgcgccgaga tcttgaccga
      481 tctgctggca cgcgcgacgt cggacgagca agtcttcatt cggcgcatct ttaccggaga
      541 gatgcgccac ggcgcgctcg aaggcgtcgt tgccgccgcc gtcgccaaag cagccggcgt
      601 ccctgccacc gtcgttcgtc gcgcaacgat gctgtcgggt gatctgtgcg cgaccgcgcg
      661 aatcgcactg acggaaggac gcgacggact cgaacggatc ggattgacgg tgcttcgccc
      721 ggtgctgccg atgctcgcgt cgacggcaga gacggtccag gacgcgctga cgttcgacgg
      781 acctgccagc gtcgaatgga agctcgacgg catccgtatt caagcgcatc gcgacggcga
      841 tgaggtccgg gtgttctcac gcaacggaaa cgatttgacc gaccggcttc cgggcgtcgt
      901 caccgttgtg cacggcttgc cggtgcgagc cgtcgtgctc gacggcgaag cgatcggcct
      961 ggacgagtcg gagcgcccgt acgcgttcca ggacacgatg agcggcacgg gccgccgcac
     1021 ggaagcattc ggcgcggtcc cgttcttctt cgacgtgctg cacctggacg gcgaagacct
     1081 gctcgacctg ccgttagtcg agcggctcga cgccctcgaa cgagtcgccg gccaccgtcg
     1141 tattcgctcc accgtcaccg aggacgcggc cgaggcgcaa cgcttcctcg acagcgcgct
     1201 cgccgcgggc catgaaggca tcatggtcaa ggatttgtcg tcgccgtatc aggccggccg
     1261 ccgcggaaag gcgtggcgga aggtcaagcc cgccaaaacc ttcgaccttg tcgtgctcgc
     1321 cgccgagtgg ggccacgggc gacggcgcgg atggctgtcg aaccttcatc tcggcgcgct
     1381 cgatgacaac gggacacccg tgatggtcgg caagacgttc aaagggatga ccgatgcgct
     1441 gcttgcgtgg cagaccgaga agctgcagtc gatcaaggag cgtgaggagg gcatcaccgt
     1501 ttgggtccgg cccgatctgg tcgtcgagat cgagctcgaa ggcgtgcaga catcgacccg
     1561 ctatcccggc ggcatcgcgt tgcgcttcgc acgactgctg cgctaccgcg acgacaagac
     1621 cgccgccgac gccgacaccc tcgaatccct acgagcgctt cagccatagc cgggcgatct
     1681 tcacgttcct acctctggtc ccctgcctca cgtcgccgcc cccgtatcgg cccggcacga
     1741 gccctccccg gtggttgtgg cgaaataatt gcggaggctt tcggccgtaa ccttcggcgt
     1801 gcggcctaga tcgaaccaat gtctaatagt cggtgccaag ggggaggaca tggaccgcga
     1861 aggatccgcg acgggcacgt cggccgcaat cgtcggtagt gccgtagggg cgtatgcggg
     1921 ctttttcgtg ttcggggcca ttggattcgg cgtcgcgagc ctgattatgg ccggcgcgcc
     1981 cgagtgcaca gaaaagctct gcggcctcgg ccaggccttg gccgctgtcg tatggggaat
     2041 cgtcatcgcc gccgtgggcg cgatcaccat gatgctgctc gggtgccgct atgcgctcgt
     2101 caagaagggc ttcgaccgtg tcggcccgac ccttgtgtgg ctggcgttcc tcgcgccggc
     2161 catcttgatc gccggcgcgg caattcttcc cgatgcctta agcgcagcct gggtcttcgc
     2221 gacaccattg atggctgctc ccgccgcccg ccgcgtcgca acgaagcggc gcggaaagcc
     2281 tgcgcagctg gccggctgat cgatccatcc cctgaccctg agcgaactcg ttcgagcgga
     2341 ccgtcacgac ggaaacattt gtccggcccg cgacgagcgg tcctgccgac gccgacacgc
     2401 tggagtctac gggcgctgcg aacgaaccga gagctgtggt gcgttagacc accgggtagc
     2461 tggtgcaaag gaagctaccg ctttgcatgc cggtgacgac gcaccgaagc acggccccga
     2521 ccgtcagctc ggcctgcggg tcactgggca atctcgtgct cgctgcccca cacgacaccg
     2581 aacggccgtc ggacgtagaa gcggcgcacg agtgcaggac ggtgccggtc accggctcca
     2641 cgatgctcac ggtcactcgc gccccggtcg tagaggatgt cgccgacccg cccgcgtaga
     2701 aggcgccccc tgcgaaaacg aagttgcacg cgccggcgtt cgtccaggag ccattgcacc
     2761 aggagatcac cgcggccccg gccggggccg ggacggccat cgaagcggcc agcatcaaag
     2821 cggcggcggt tcgggcaaaa cccttcatga tggtctcctt ccgtcaagcg gatcgggcgc
     2881 tcaataacgc aacgattcga taccgagagc gcttgtcctg cccccggcag aaacgaaccc
     2941 acgggctttc gaagtccgac acatccccct tgaccgcatc catcggtttg tgagtagatg
     3001 tctgtgatgc ggtcgcctgc gaaagggcac acggcgacct tcgagcagac gctttcgttg
     3061 atggccgaca tcaccgacga ggacggcgag gtcgccgaag aggcgatccg ctgggagtcg
     3121 gacctcaacg gctttctcgg aaccggcgaa gacgtagcca tcagcgggct caaggaaggc
     3181 acgcacacca ttcgcgtcac tgccgacgac ggccgtggag ggatggcggc agacgaaatc
     3241 acggtgagga ttgtcagaga ccccactgcg ctgcctgcca cacccagtcg cgtgacggcc
     3301 tccccgtcga tgctccaatg cgatccgtcc gagcagctca tgcagcatga cgtcgtcgtc
     3361 gaagacgaga actacatgct tccggccaaa gtgttccggg ccacggcgtc ggagccatgg
     3421 gtcaagctca tgcctgttcc ccgggggcag gacgtcaggg tctcggctcc gtacggggcc
     3481 atgtccgttg ccggactgac gcctttctcg ttgcgggtga catgcgaagg tgtcgggaac
     3541 gtgtcgcaag ggattcactc cgcggttgtg acgatcgatc tggtcccgag cggccggctt
     3601 accgtgcctg tcacattgcg gacgacgtag cagccaacac gtcgggatgc ccgcggggct
     3661 tcttcagccc gcgggcatcc cggccgcctg agaacgaacc acggccgtcg gatcaagatg
     3721 cgggtcaacc cacagcgacg gtgccgacaa cgacaccgtt tcgcaccaac gtgatgacgg
     3781 ccggtgtgtc gctcgtcacg tcgatcaccg gcgtatctga caggccggct cgaccgacgt
     3841 ccaccgtgac cgacgtcacc ttcgacaacg aaccgctcag cgtggcggtc gcggcaaccg
     3901 ccgcaaccgt gcggtcctga ccggtgagca gatagggaga cggcccgtcg gcgcccacgg
     3961 tcgcgaccgg gctcacgccg gtctgctgca tgatgacgcc gccactacgc agatccacga
     4021 tgccggtgac atcggcgccg ctcgcgacct ggacattgcc gacccagtag gcgccgtcga
     4081 gacgcgcgcc gacctcgctc acgagcgcgt tgacgtcggc gagcagcgcc gcagcgtttg
     4141 gcttcgtgct ggttacccag ctcgccgggc gtaccttgag cgtgacgcga tccgggttct
     4201 gcgcgcgcgt ggggtgcgcg gcgagccagg aagcctcgcg cgtccattcg tcggcaaagg
     4261 cgaatgcgaa gtgatcggcg gcagggtgca gcgcgtagcg gtaggtgtgg ccgagctctc
     4321 cgaggcgcat ggcctgacgc gtcacgccag agaccggcac gagctcgtcg agcatgccgt
     4381 tggtgagcca gtacgggata tggcgcgtgt tctcgagctg cgggaaggtg tggtcggggt
     4441 tggactcgcc gccggtgggc ggcagcggaa acgcccagat gccgttggcc ggcggtgcca
     4501 ccacggccgc ggcggacgcg aacgcgtcgg gtatgagggt gccgagccgg tacgtgccgt
     4561 agccccccat cgagtatccg ccgatgtaga cgcgatcgcg gtcgatcgta aatagatcga
     4621 gcgcgtcctt ccatacctcg agcgtgtcga cgagcgcctc gtcttcatac cagccgtcgg
     4681 gtccccggcc gagcggcgtg acgatgatcg cgccggtctt cgcctcgtac tgctcgtaca
     4741 ggctgcggta cgtcgccgac ggggccgcgc cgccccggta ttggttgtgg tgcaccgaca
     4801 agctgtgcat gttgaggaac agcggcaccg gggttccggg cacgtaggac tccgggaccc
     4861 agatcgcata gggctggaag cggcccagat actgcgggaa ggccgtaccg atgccttcgt
     4921 tcgcaccgag ctgctggcgc gactcgaaca ggcgtacgtg gtaggtgcgc acgtcgggga
     4981 ccggcgtcgt cgccgcagcc ttcagcgcgg tcacgtcgat cgtctgcacg aacggcccga
     5041 ggtcgcccgc atggatcgcg tcggactgga cgtcctcatt ccaggcgccg ccgcggctct
     5101 caatcgcgtg cgtgttgaac gcaaggtcga acagtcgcgc gttcgtcggg gcgccgctgg
     5161 tgaactcgct gggcgttgcg tttgcagagc ccggaaccgg cgcgcgcagc tcgcccgatc
     5221 cgttgttcag gcccgcgccg atgttgagaa cgaccgcgtc gtcggccgcg gcgccggcga
     5281 gcgtgaacgt caccgtgttc gccgccgcgt tcacgactgc gttgtccgtc gtgacgatcg
     5341 tctcgacgtc gttcgcagac acccacagcg ccaccacatc cggatcgacg atcgtctgca
     5401 acagcacgcg aacctctagg tcggagccgt tcggcgcgac gcgcgtgcgc acctcgacga
     5461 catcggcggc gttgccggcg tacgccggcg tgcggggata gcgaaagtcg ccgctcgtcg
     5521 acgcgaggct cacgatgttc ggctgaccgg ccggagtcgt gtccgcaccg tagtcgtcgt
     5581 agacgaagtc ggtgtagatc cattcgcccg cgtcgtagcg cgacgtgccg gcgatcatcg
     5641 tcggcgtccc ggtccaggct gcggtactcg agaccgggct cgcgatcaac gcggaatcgg
     5701 ctgcgaccgc gtggccggtc ggggcgaggt cggcaccgac ctgcgcaccg gcgggaaggg
     5761 aggcgagtgc ggcgaccgcc gcgaagatcc atccgagctg aagacgtcga gacattgccg
     5821 ggacccccgt ttcgttttgc gaatttccct gatgattcca cgacgagccc tcgatccctg
     5881 ctcgcgggcc gagctcgaac attttcgtgc ggctcgggcg gggggcgagc gccgggagcg
     5941 gccgctcagc ggtacgggtg ctggaccacc atacgaatca tggcctcgcc cacgctcttg
     6001 atcggaacgt tccgcaccag ccagcgcgct tggtcgaacg tgcccgcacc gagccggccg
     6061 gctgctttct cgtccggcac atggatgccg agaccgagat gcgcgcctaa ccgggtggcg
     6121 tcgattggcc gcatcgattg cccgcaaggt ttcgcgtctg gttccggtcc tgctcgccgc
     6181 aggtttatgg gcgtctccag tcgcaatggc ctcgccggtc gatggtggcc gtgcgcggcg
     6241 catcttccgc gtccgggcgc gcgccggacg cagcggtctg ttcattcgag cacgagccgg
     6301 tggtcgcacg ttccgcttcg gccggcccgc ggacggggtc gtgcgcgcgc gaatccgggc
     6361 cgatcgcacg tgctcttcgc gcatcgcctg catcgcacgc acgcggttcg ggggcgccgt
     6421 cgacggatcc ggcgtggcaa agggaacgcg cgttcgcgcg aacggcgtca tccgagccgg
     6481 catccgcgca ggctcggcca cgcatcccgc gccgacgccc gtgtatcccg tcgcgagtgg
     6541 ccgaacgacg atccgcgcac gtgcggcggg ccagcgagtc gagcggaccc tatcgatccc
     6601 tccgcagatc aaagtgcctg cagccgaaag gcttcccgta gatctgcgga ccggacagcc
     6661 gctctagctc cggacgcgtg ctcgggtcag gtatctcggt cgacgtaacc attcagtggt
     6721 caccgcgatc tagctccgtc gatcccttcg aaaaactgtg gcgctccctt ccgc
//
LOCUS       DATLFO010000240         7929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_123628, whole genome shotgun sequence.
ACCESSION   DATLFO010000240 DATLFO010000000
VERSION     DATLFO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123628"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..786)
                     /locus_tag="VM841_09770"
     CDS             complement(280..786)
                     /locus_tag="VM841_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVL90505.1"
                     /translation="MAQSDVLLEELTLLSRDFFRLISDCLTRHADLAEISPTQFRALA
                     FLVQRGPHNASDLAETLAVGRPAATKLVDRLVRSGMIRREPHPTDRRQVILEATGEGF
                     EVVQAVQRCRTRRLAAVLGAIEPASRKALAKALPALTDALAAAAEDSAEAAESVAKRP
                     AKGGKRRS"
     gene            887..2248
                     /locus_tag="VM841_09775"
     CDS             887..2248
                     /locus_tag="VM841_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90506.1"
                     /translation="MESAPHRESGPAGGRVLTGREPETVLEPDPIPSAPAPAPAAGIE
                     APAVPRLLRRGPLSPLRHRDFALFWSGAFASNAGTWLQNVALAWLVLEITDSPFWVSM
                     VTFTQFFPMMLLGLVGGLVADRFERRRILLWSQGGMMAVAVALAAITFGGYASVATVL
                     PVVALGGIVLAFNAPAFQAIIADLVPEDALLDAVSLNTAQFSAARVIGPLLGGITLAT
                     VGAGWAFAANAASFSFVIAALLLIRPRRHAPPASRGARALFGGFRAMRDFPVIKTIVA
                     LVSVLSLFAAPVLSLLPVIAKNVLGLGATGYGGLFAAFSAGAVAGALLTGRVVRRLGV
                     RQAVGAGVGVLGASTVILALSGNVALSAVSLVAIGWAYTSTVSTTNTGLQIAIPATKR
                     GRVMSLYMMAWAGLYPVGSLLGGIAATHVGVRSTLVVAALPLLAAAAWARLRGGGLRN
                     LRV"
     gene            complement(2245..2733)
                     /locus_tag="VM841_09780"
     CDS             complement(2245..2733)
                     /locus_tag="VM841_09780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90507.1"
                     /translation="MPAHPVPRLTIATTPRHFEIFTELAREYVASLDFPLTFQDFERE
                     IAGAAAEYGPPGGRAWIAFAGDEPAGIVGLRAFAADTAEIKRMFVRPAHRGIGAGRLL
                     AAAAVGGARVLGYRRIVLDSIRAMTAALAIYRSLGFVEIEPYRYNPRPDAVYMALDLS
                     EA"
     gene            3045..4355
                     /locus_tag="VM841_09785"
     CDS             3045..4355
                     /locus_tag="VM841_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL90508.1"
                     /translation="MSLASQDAASPGIGSLGIRLRAARRSKGLTQADLAGPDYSVAYV
                     SRIESGERTPSPEALATFARRLGVSLDSLLSPIDDAAMLEIERARIAAARGIDEADWD
                     AARAAATELLQLAERHALPRFASIANRVLGELEERVGELDRALARYTEAERTVSDDVE
                     DRVDMTVCLARVHRRFGDLGYSADLLTRAIAALEPEAELHPALMARLNLHLSATCSER
                     GDYARARRAGLEAIEWARKAGDTRTLANALWAAAPPMAVANPARALSLIRRAGTLFAE
                     LGLTHELGRLQAMLGETALTAGDEPTARVALERASELIGPSAPPSVLSTILVSKAELE
                     RRDGNVDEAVRLARQVVDIAEGVEPLEQARARRVLGESLVTQDPEAAERELRAAIVLF
                     TSAGAQMDAARCFRSLGDLLLALGRTDEAVAVFRDGLGAVEGAA"
     gene            4403..5215
                     /locus_tag="VM841_09790"
     CDS             4403..5215
                     /locus_tag="VM841_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90509.1"
                     /translation="MEQVAELSSATLAYEIVGSGPPLVWCHGLASCREGDRDVIDAFA
                     RHFTVLGYDARGHGRSAPARIEEDFTYPQLSRDLRELLEHVGWDRAILTGASMGAATA
                     VRVAMEDPKRAAALVMVRPGSDGGPAPQHLQMLFRLGASAIRSGGLDAAIDFLMTIPE
                     ARAAIEKDPKRLAQLREDWGRHDPLSIAAALEGIPASPPLSGEIDPRDILAPALVIPG
                     NDPIHSLEAGMAVAALIPTARAAEPLEAVSREEETERLLEIVLAFLREHLLD"
     gene            complement(5212..6057)
                     /locus_tag="VM841_09795"
     CDS             complement(5212..6057)
                     /locus_tag="VM841_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HVL90510.1"
                     /translation="MKASYVLVDVFSDRPLAGNPLAVFPDGHDIPEELFAPVARELNL
                     SETVFVTAIDESGYDARIFTPGAELPFAGHPTLGTAWVLSHLGRIEGPWLVQRTAAGD
                     TPVSLAFDTVWFERTGTVGRDIEDTSFIAPALGLAESDIGFNAGVLGYSRRWLAPAFA
                     DAGVEQLMVPVADRETLARMRPRPDVVDLSGQGVYCFTALAPTKLKARFFAAGIGIAE
                     DPATGSAAANVGLYLADRLGDLAFDIKQGKETGRPSTLSVDTSRGRVRVGGQVVLVGE
                     GELLL"
     gene            6160..6966
                     /locus_tag="VM841_09800"
     CDS             6160..6966
                     /locus_tag="VM841_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HVL90511.1"
                     /translation="MQPSKQEVIGAVAAQRARTIALVEPLTAAQWDAPALPGWRVREV
                     AGHLVASDQGALTGRMLRVGMRPQPDGGLEAVEAWNEAEVHRWSDRPAAEIVEGLRTW
                     GRRSVALFKATPATILRRTVPLPFGKVPLLFLGQIRVFDEWVHEQDIRRALQMDPGYD
                     LPSLTAAARALLAVMPLQTPVRMPADTKGTLSVRFDGIDVPPLFVDLLTKEFGFDGGN
                     VDATVTGTAPALIFSAANRDAWRDHERGGTIRIDGDRAVGEAFLDAMRAG"
     gene            6966..>7929
                     /locus_tag="VM841_09805"
     CDS             6966..>7929
                     /locus_tag="VM841_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVL90512.1"
                     /translation="MGELADLAARIASGETSSLAEVGRTVETIEHAQHAINLFTVIAR
                     EHAIAAAEEADASTPRGPLHGVPVAVKDLFDVAGWPTTACSKLYANRVATADAPVVAA
                     LRAAGAIIVGKTNMHELALGATGDASSIGPADNPWNPSHMTGGSSSGSAAAVAAGLVP
                     LTLGSDTGGSVRIPSSLCGVSGLKTTNGLLSVQGAVPLSPTLDTAGPIAGDAEDLALA
                     MNVLGGMSVAWIVDCAGLTVGLAQNRSFDTIDPLVDAGVRAAVAVLEAAGATVVDIEL
                     PDAERARDTWTDIVLPEFLRAHPGMDEALLSDELRFLAGAARTIG"
BASE COUNT         1111 a         2815 c         2802 g         1201 t
ORIGIN      
        1 gtcccggacg atcccgcggg ccgcagcggc ggcccgcacg ggcgacgaca gcgccgcccc
       61 gcggccgggc cggaaatgag aatcgcgccg gcccggctgt ctaacccccg tcagtcagac
      121 gaaccataac cggcgcgata ccgactccgt atccatggaa gaacggctcg gagacgccgc
      181 gattctaata ccggcaggag cgtccggaaa gccgagcggt gcgggcaaag cggacaataa
      241 gccctttaac ttcactaaag aattcgtgag ccggaaaggt cagcttcgcc gcttgcctcc
      301 cttggccggc cgtttcgcga cgctttccgc ggcctcggcc gaatcctccg ccgcagcggc
      361 cagcgcgtcg gtgagcgcgg gcagcgcctt ggcaagcgcc ttgcgcgacg ccggctcgat
      421 cgcgcccagc acggccgcca gccgccgcgt gcggcaccgc tggaccgcct gaacgacctc
      481 gaagccctcg ccggtcgcct ccaggatcac ctgcctgcgg tcggtgggat gcggctcgcg
      541 ccggatcatg ccgctgcgca ccaagcggtc caccagcttg gtggccgccg ggcggccgac
      601 cgcgagcgtc tcggcgaggt cgctcgcgtt gtgcggccct cgctgcacta ggaacgccag
      661 cgcgcggaac tgggtagggg agatctcggc aaggtcggcg tgccgggtca ggcagtcgga
      721 gatcagccgg aagaagtcgc gcgagagcag cgtcagctct tccagcagca catcggactg
      781 cgccacacga gttcctttcg cttcggcggg aacaacgcgt tcgccgcccg gcgttgccaa
      841 tggtacggtt tccaacggaa acaatcgagg agggacggac agcacggtgg agtcggcgcc
      901 acaccgggaa agcggccccg caggaggccg cgtcctgacc ggccgggagc cggagacggt
      961 tctcgagccc gatccgatcc cgtccgctcc cgcgcctgct cccgccgccg ggatcgaagc
     1021 gcccgccgtc ccgcgcctgc tgcgccgcgg cccgctgagc cctttgcgtc accgggactt
     1081 cgctttgttt tggagcggcg cgttcgcgtc caacgccggc acgtggctgc agaacgtggc
     1141 gctcgcgtgg ctcgtgctcg agatcacgga ctcgccgttc tgggtctcga tggtgacctt
     1201 cacccagttc ttcccgatga tgctgctcgg cctggtcggc gggctggtcg ccgaccgctt
     1261 cgagcggcgc cgaatcctgc tgtggtcgca gggcggcatg atggcggtcg ccgtcgcgct
     1321 cgcggcgatc accttcggcg gctacgcgag cgtcgcgacc gtgctgccgg tggtggcgct
     1381 cggcggcatc gtgctcgcct ttaacgcgcc tgctttccag gcgatcatcg ccgacctggt
     1441 tcccgaggac gcgctcctgg acgccgtctc gctcaacacc gcccagttct ccgcggcccg
     1501 ggtgatcggg ccgctgctcg gcggcatcac gctcgcgacc gtcggagccg gctgggcgtt
     1561 cgcggccaac gccgcctcgt tcagcttcgt catcgccgcg ctgctgctca tccgcccgcg
     1621 ccgccacgcg cctcccgcct cgcgcggcgc gcgtgcgctg ttcggcggct tccgcgcgat
     1681 gcgcgacttt cccgtcatca agacgatcgt cgccttggtg tcggtgctca gcctgtttgc
     1741 cgcgccggtt ctgtcgctgc ttccggtgat cgccaagaac gtgctcggcc tgggtgcgac
     1801 cggctacggc gggctgttcg cggcgttttc ggccggcgcg gtcgccggag ccctgctcac
     1861 cggccgggtc gtgcggcggc tgggcgtccg gcaggcggtc ggagccggcg tcggcgtgct
     1921 cggcgcgtcc acggtgatcc tcgcgctgtc gggaaacgtc gcgctgtcgg ccgtgtcgct
     1981 cgtcgcgatc ggctgggcct acacgtccac ggtctccacg accaacaccg gcctgcagat
     2041 cgcgatcccg gcaaccaaac gaggccgggt catgagcctg tacatgatgg cctgggcggg
     2101 cctgtatccg gtggggtcgc tgctcggcgg gatcgcggcc acgcacgtgg gcgtacgcag
     2161 cacgctggtg gtggcggccc ttccgctgct cgccgccgcc gcctgggcgc ggctgcgcgg
     2221 cggcggcctg cgaaatctgc gtgtctaggc ctcgcttaga tccagcgcca tgtagacggc
     2281 gtccggccgc gggttgtagc ggtacggctc gatctcgacg aatccgagcg agcggtagat
     2341 cgccagggcc gcggtcatcg cgcggatcga gtcgagcacg atgcgccggt agcccaggac
     2401 ccgcgccccg ccgacggccg cggccgcaag caggcggccc gcgccgatgc cgcgatgagc
     2461 cggccgcacg aacatgcgct tgatctcggc ggtgtccgcc gcgaaggctc gcagcccgac
     2521 gatgccggcg ggctcgtcgc cggcgaaggc gatccacgcg cggccgcccg gcggtccgta
     2581 ctccgccgcc gccccggcga tctcccgttc gaagtcctgg aacgtcaagg gaaagtcgag
     2641 cgaggcgacg tactcacggg cgagctcggt gaagatctcg aaatgccgcg gcgtggtggc
     2701 gatcgtcagg cgcgggacgg gatgggcagg cacgacggaa ccggggaggc ggagcctcca
     2761 tccgctagga gcgcgggatc agtagggcca ctgaccgttg ccggtgccct tggccgccaa
     2821 aagggcggca gctatcaggg tcagggtcgg agccaggcgg cgaatcgttg caagcagacg
     2881 ggacttgcgc atcgttcccc ccacggagcg gacttcggtc gatcttacga cagcccgacc
     2941 ctttcggaaa ggtccggcgc gatccgggcg ggcggttcgc cggatcgcgc ttcggtgaag
     3001 ccagtattga caccggcctg tttctctgtc aaatttttca gtcaatgagc ttggcttcgc
     3061 aggacgcggc ttcccccggc atcggatccc tcgggatccg gcttcgtgcc gcacgcaggt
     3121 cgaagggttt gactcaggcg gatctcgccg gccccgacta cagcgtcgcc tacgtcagca
     3181 ggatcgagtc gggggagcgg accccgtcgc ccgaggcgct cgccacgttc gcgaggcgcc
     3241 tcggtgtttc cctcgactcg cttctttctc cgatcgacga cgccgcgatg ctcgagatcg
     3301 agcgtgcgcg catcgccgcc gcccgcggaa tcgacgaggc cgactgggac gccgcccgcg
     3361 ccgccgcaac cgaactgctg cagctggccg agcgtcacgc gctcccgcgc ttcgcatcga
     3421 tcgccaaccg cgtgctcggc gagctcgaag agcgtgtcgg cgaactcgac cgtgccctgg
     3481 cccgatacac cgaagccgag cggaccgtct ccgacgacgt cgaggaccgg gtcgacatga
     3541 ccgtgtgtct ggcgcgcgtc caccgccgct tcggcgacct cggctactcg gccgatctgc
     3601 tcacgcgagc catcgccgcg ctcgagcccg aagccgagtt gcatcccgcg ctgatggcgc
     3661 gtctgaacct tcatctttcc gcgacgtgca gcgagcgagg cgactacgct cgcgcgcgcc
     3721 gcgccggact cgaagcgatc gagtgggccc gcaaggccgg ggacactcgc acgcttgcca
     3781 atgccctctg ggctgccgca ccccccatgg cggtggccaa ccctgcccgc gcgctctccc
     3841 tcatccggcg cgcgggcacc ctttttgccg agctcggcct gacccacgag ctcggacgcc
     3901 tccaggcgat gctgggcgag accgcgctga ccgccggcga cgagccgacg gcgcgggtcg
     3961 cgctcgaacg cgcctcggag ctgatcggcc cgtcggcgcc gccgtcggtg ctgtccacca
     4021 tcctggtgtc caaggccgag ctcgagcgtc gcgacggcaa cgtcgacgaa gccgtacggc
     4081 tcgcccgtca ggtcgtcgac atcgccgaag gcgtcgagcc gctcgagcag gcgcgagccc
     4141 gtcgcgtgct cggcgagtcg ctcgttacgc aggaccccga agccgccgag cgggagctcc
     4201 gtgccgccat cgtgctcttc acctctgccg gagcccagat ggatgcagcg cggtgcttcc
     4261 gctcgctcgg ggatctgctg ctcgccctcg gccgcaccga tgaagcggtc gcggtcttcc
     4321 gcgacggcct cggcgccgtc gaaggcgccg cgtagccggc ttgttcgccc ctccctccgc
     4381 gcggcggtat cgtttcgcaa cgatggagca ggttgcggag ctgtcgtcgg ccacgcttgc
     4441 ctacgagatc gtcgggtccg gcccgccgct ggtgtggtgc cacgggctgg cctcctgccg
     4501 cgagggcgac cgggacgtca tcgacgcctt cgcgcggcat ttcacggtgc tcggctacga
     4561 cgcgcgcggc cacggccgca gcgctccggc ccgaatcgaa gaagacttca cctacccgca
     4621 gctgtcgcgc gatcttcgcg aactgctcga gcacgtcggc tgggaccgcg cgatcctgac
     4681 gggcgcctcg atgggcgcgg cgaccgccgt gcgcgtcgcg atggaggatc ccaagcgtgc
     4741 ggccgcgctc gtgatggtgc ggccgggctc ggacggcgga ccggcgccgc agcatctgca
     4801 gatgctgttc aggctcggcg cctccgcgat ccgaagcggc ggcctggatg ccgctatcga
     4861 cttcctgatg acgatccccg aggcgcgcgc ggcgatcgag aaggatccca agcgtctggc
     4921 gcagctgcgc gaggactggg gccgccacga cccgttgtcg atcgcggccg cgcttgaagg
     4981 gatcccggcc tcgcctcccc tgtcgggcga gatcgatcct cgcgacatcc ttgcgccggc
     5041 gctggtgatc cccggcaacg atccgatcca ctcgctcgag gcgggcatgg cggtcgccgc
     5101 cctgatcccg acggcacgag cggccgagcc gctcgaagcg gtctctcgcg aggaagagac
     5161 cgagcggctg ctggagatcg tgctggcgtt cctgcgcgag cacctgctcg actagagcag
     5221 cagctcgcct tcgccgacga gcacgacttg accgcccacg cgcacccggc cgcgcgaggt
     5281 gtccacgctg agcgtgctcg gacgcccggt ctcttttcct tgcttgatgt cgaaggcgag
     5341 atcgccgagc cgatcggcca ggtacagccc gacgttggcg gccgccgagc cggtggcggg
     5401 atcctcggcg atgccgatgc ccgcggcgaa gaagcgcgcc ttgagcttgg tgggggcgag
     5461 cgcggtgaag cagtacacgc cctggcccga cagatcgacc acgtcgggac gcggccgcat
     5521 ccgcgcgagc gtctcgcgat cggcgaccgg gaccattagt tgctcgacgc ccgcgtcggc
     5581 gaaggcgggc gcgagccagc ggcggctgta gccgagcacc cccgcgttaa acccgatgtc
     5641 ggactcggcg agcccgaggg cgggggcgat gaaggacgtg tcctcgatat cgcggccgac
     5701 cgtgccggtg cgttcgaacc agaccgtgtc gaaggcgagc gagaccggcg tatcgcccgc
     5761 ggccgtgcgc tggaccagcc acggcccttc aatgcggccg aggtggctga gcacccaggc
     5821 cgtgcccagc gtcggatggc ccgcgaacgg aagctcggcc cccggcgtga agatgcgcgc
     5881 gtcgtagccg ctctcgtcga tcgccgtgac gaagaccgtc tcggacaggt tcagctcgcg
     5941 cgcgacgggc gcaaagagct cttccgggat gtcgtggccg tcgggaaaga cggccagcgg
     6001 attgccggcg agcgggcggt cggagaagac gtcgacgagg acgtagctgg ctttcatcgg
     6061 cacgccgaca ttaaggagcg cgtgtcctca ttgcaagcgc gagcgatagg ctggtcccat
     6121 ccggcctcgc caatggccgg cggaaggatc gggggaggca tgcaaccgtc caagcaggag
     6181 gtcatcggcg ccgtcgcggc gcagcgcgcc cgcacgatcg cgctggtcga gccgctgacg
     6241 gccgcgcagt gggacgcgcc cgcgctgccg ggctggcgcg tgcgcgaggt cgccggccac
     6301 ctggtcgcca gcgatcaggg ggccctgacg ggccggatgc tgcgtgtcgg catgcgtccg
     6361 cagccggacg gaggactcga ggccgtcgaa gcctggaacg aggccgaggt tcatcgctgg
     6421 tcggaccggc cggccgccga gatcgtcgaa ggcttgagaa cttggggacg ccgaagcgtc
     6481 gcgctgttca aggcaacgcc ggccacgatc cttcgccgca cggtgccgct gccgttcggc
     6541 aaggtgccgc tgctgttcct cggccagatt cgcgtcttcg acgagtgggt gcacgagcag
     6601 gacatccgcc gcgcgctcca gatggatccg ggatacgacc tgccctcgct gacggcggcc
     6661 gcgcgcgcct tgctcgcggt gatgccgctg cagaccccgg tgcgaatgcc cgccgacacc
     6721 aagggtacgc tctcggtccg cttcgacggc atcgacgtcc cgccgctgtt cgtcgatctg
     6781 ctcaccaagg agttcgggtt cgacggcggc aacgtggatg cgaccgtgac cggcaccgct
     6841 cccgcgttga tcttctcggc ggcgaaccgg gacgcctggc gcgaccacga acgcggcgga
     6901 acgattcgca tcgacggtga tcgcgccgtg ggggaggcgt tcctcgacgc catgcgcgcc
     6961 ggctgatggg cgaactggcc gatctcgccg cgcgcatcgc ctcgggcgag acctcgagcc
     7021 tcgccgaggt cggacgcacg gtcgagacga tcgagcacgc gcagcatgcg atcaacctgt
     7081 ttacggtaat cgcacgcgag catgcgatcg cggcggccga ggaggccgac gcatccacgc
     7141 cccgcgggcc gttgcacggc gtcccggtcg cggtcaaaga tctgttcgac gtcgccggct
     7201 ggccgaccac cgcgtgctcg aagctgtatg cgaatcgcgt cgcgaccgcg gacgcgcccg
     7261 tcgtcgcggc gttgcgcgcc gcgggcgcga tcatcgtcgg taagaccaac atgcacgagc
     7321 tcgcgctcgg tgcgaccggg gacgcctcgt cgatcggtcc ggccgacaat ccgtggaatc
     7381 cgtcgcacat gaccggcggc tcctccagcg gcagtgcggc ggcggttgcg gccgggctgg
     7441 tgccgctcac gctcggctcg gacaccggcg gcagcgtgcg gatcccgtcg tcgctgtgcg
     7501 gcgtcagcgg cctcaagacc accaacggat tgctgtccgt ccagggcgcg gtgccgttgt
     7561 caccgacgct ggacaccgcc gggccgatcg cgggagacgc tgaagatctg gcgctcgcga
     7621 tgaacgtgct cggcggcatg agcgtcgcat ggatcgtcga ctgcgcgggc ttgaccgtcg
     7681 ggctggcgca gaaccgatcg ttcgacacga tcgatccgct ggtcgatgcg ggcgtgcgcg
     7741 ccgccgtcgc cgtgctcgaa gccgccggcg caacggtggt ggacatcgag ctgcccgacg
     7801 ccgaacgcgc gcgcgacacc tggaccgaca tcgtgctgcc cgagtttctg cgcgcccatc
     7861 ccggcatgga cgaggcgttg ctctccgacg agctgcggtt cctcgccggc gccgcccgca
     7921 ccatcggcg
//
LOCUS       DATLFO010000241         6765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_4886, whole genome shotgun sequence.
ACCESSION   DATLFO010000241 DATLFO010000000
VERSION     DATLFO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4886"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="VM841_09810"
     CDS             complement(<1..1800)
                     /locus_tag="VM841_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HVL90513.1"
                     /translation="MSSEEAGAGQDEAFEALLHFLKQTRGFDFTGYKRSSLIRRVRRR
                     MDAVGVFEFAAYTDFLQVHPEEFMHLFNTILINVTSFFRDAQAWDYVREHIAPALTES
                     DRPIRVWSAACASGEEAYTVAMVLAERLGESGFLERVKIYATDADEEALNQARLGSYS
                     PRDVEGVPEELREKYFQRTDHAFVFRKDLRRAIIFGRHDLVQDAPISRIDLLICRHTL
                     MYFNADAQAKILTNLHYALNPEGYLFLGRPEMLLTHTELFQPLELKKRIFRPVPRGHA
                     RERMDIPRREFVPPLLPVGEDLRQAAFEASRSAEIVVDNDGHLRLVNAHARAWFGIPP
                     SDVGRPLRDLEFSYRPVELRSLIDRAVSEDRPVTARDVLWVGPAGDQRHLDVDVIPLL
                     DAHGESAGVMVSFTDMTRYRRLQDELQTSKRDLETAYEELQSTVEELETTNEELHSTN
                     EELETTNEELQSTNEELETMNEELQSTIEEMETINQELEDRTTELNQSNASLESILAG
                     IRLGVIVADRDLRILAWNRRAEDLWGLREAEVRGEHLLNLDIGLPLEQLRKPIRTVLS
                     HAEQQDLVLDAVTRRGKSIQCVVKCAPRSGAHLT"
     gene            complement(1800..2774)
                     /locus_tag="VM841_09815"
     CDS             complement(1800..2774)
                     /locus_tag="VM841_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HVL90514.1"
                     /translation="MRGLVVVGASAGGVDALARLASGLPDDLDAAVLMVLHVSHGVRS
                     AMPAILTRAGRLPAEHPRDGEAIRPGRIYVAPPDRHLMVSGERLLVTPGPKENGHRPA
                     IDPALRSAAEWWGPNVMGVILSGTLDDGATGLAEVRRAGGVTVVQSDALFPHMVESAR
                     HLVTPDYDVPLAEIPVVIARWAREPRTGEGATGMTGEADPRPVPVAHTCPECGGTLWE
                     TEAGNGFRCRVGHVFSDKALLAANGEQVEAALWTALRSMEERRDLFRRIAVRNEERRP
                     WLAKRYRERSEEIDDQRLVLQSAVADAIAVSGVSTEDSPEPATQPEGD"
     gene            complement(2902..4275)
                     /locus_tag="VM841_09820"
     CDS             complement(2902..4275)
                     /locus_tag="VM841_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVL90515.1"
                     /translation="MSDLAEALQQILPAEHVLTGDEITEDYTHDETLSAEAAAPAVVV
                     RPGTAGEVAAICALANERGTPVVGRGSGTGLSGAAIPPSDAILISFERMNAIVEIDTE
                     NHVAVVQPGVTLAQLDEALAPFGLVYPVHPGETSGSLGGNAATNAGGMRAVKYGVTRN
                     QIVGLQCVLADGRIIRTGGKLAKSSTGYDLTQLIIGSEGTLALVTEAILKLHPRLPHR
                     ATVLAPFRTLEEVTRAVPRVVAGGAGPMILEYIDAFTMAAITAHVGLDLGIPEDVKQS
                     ATAYLVVVLESAKPDRLDEDVAEVGELVTGLDAMDVYILSPLAGQQLIEAREKAFWTA
                     KAANAADLVDIVVPRASIAEFFTHVVTIAEKHETFLAGCGHAGDGNVHLSVFQPDPGK
                     RDRVMRALFEAGMSVGGAISGEHGIGRSKKKYFLELEDPAKIELMRGIKKVFDPNDIL
                     NPGVLLA"
     gene            complement(4339..6549)
                     /locus_tag="VM841_09825"
     CDS             complement(4339..6549)
                     /locus_tag="VM841_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90516.1"
                     /translation="MTLRLLLAVSLLAGVAAVAPAGAVSPPALRAPCDALDRAHCLLP
                     FPNDRFTKADPSTDTGRRVDFQVAEMPRGAGVKPIDPTEWNRNDGFSPGSPVLTFVRG
                     LDLHKTWGTAGISTGGPNDPADHIADIARYQAPDAPILIIDATTGERWPFWSELDTNE
                     FTQDNERLLILRPAKNFLEGRRYLVALRNMRNEDGLPIERGEEFTAIMERSRPVDVEQ
                     DRADHIRGVVAEVAAAEWARGNHAFEPTSLFLAWDFTVISQRSMTERVLHIRDDAFAQ
                     LGDTNLADGIVQGAAPVYSITSVTGDSGTRQRRIEGTITVPYYIDRPPFHVGPERINT
                     MVAGNVPNQAIPTGRFLYGPDGLPMQNPVIPTIEAPFVCTIPAASTAANPAHPMLYGH
                     GLLGSRNESAGSSSDRMRERNFMPCAVNWFGFAEYDVANALVTLTDPTNMASMMDRVQ
                     QGFVNFLYLGRALVHADGLTADPAFKDASGNRLAEPGKLVYDGNSQGGIMGGALTALA
                     VDHTLATLGVVGMNYSTLLNRSVDWEGPLVDPGNPGLPSYSSFLYTMFPDKKEQQVVM
                     AVLQMLWDRGEANGYAQHMTDDPLPNTPAHRVLLHVALGDFQVAPVSAEVEARTIGAA
                     FLGSQLMPGRHRSVDPGFGMPSMPAVHEGSALIIWDSGNYLEPNGNVPPILDGGDPHE
                     DPRRDPRSGDQRAHFFRTGQIIDVFNGQPYLTCRPDRTTQIPRVPSLFGFDWCV"
BASE COUNT         1048 a         2263 c         2341 g         1113 t
ORIGIN      
        1 ggtcaagtgc gcgccgctgc gaggcgcgca cttgaccacg cattgaatcg attttccgcg
       61 gcgggttacc gcgtcgagga cgaggtcctg ctgctccgcg tgcgagagaa cggtgcgaat
      121 cggctttcgg agctgctcga ggggaagtcc gatgtcgagg ttgagcagat gctcgccgcg
      181 gacctccgcc tctcgcaagc cccagaggtc ttcggcacgg cggttccagg caaggatccg
      241 aaggtcgcga tccgcgacga tcacgccgag ccggatgccc gcaaggatcg actcgagcga
      301 ggcattgctc tgattcagct cggtggtgcg atcctcgagt tcctgattga tcgtctccat
      361 ctcttcgatc gtggactgaa gctcctcgtt catcgtctcg agctcttcgt tggtggattg
      421 cagctcctcg ttcgtggttt cgagctcctc gttcgtcgag tgcagctcct cgttggtcgt
      481 ctcgagctcc tcgacggtgg actgcagctc ctcgtaggcg gtctccaggt cccgcttcga
      541 ggtctgaagc tcgtcctgga gccggcgata ccgggtcatg tcggtgaagg agaccatcac
      601 gccggcgctc tcgccgtgcg catccagcag cgggatgacg tcgacgtcga gatggcgctg
      661 gtcaccggcc gggccgaccc acagcacgtc gcgggccgta acgggccggt cttcggaaac
      721 cgctcgatcg atgagcgagc gcagctcgac gggacgatag gagaactcga ggtcgcgcaa
      781 cggacggccg acgtcggacg gcgggatccc gaaccacgca cgcgcgtggg cgttgacgag
      841 ccggaggtgg ccgtcgttgt ccaccacgat ctcggccgag cgggaggctt cgaacgccgc
      901 ttgccgcagg tcctccccga ccgggagcag cggcggcacg aactcgcgcc gcgggatgtc
      961 catgcgctcg cgggcgtggc cgcgcggcac gggacgaaag atgcgcttct tcaactcgag
     1021 cggctggaac agctcggtgt gggtcaggag catctcgggc cgtcccagga agaggtagcc
     1081 ctccgggttg agcgcgtaat ggagattcgt gaggatcttg gcctgcgcgt cggcgttgaa
     1141 gtacatcagc gtatgccggc agatcagaag gtcgatccgg gagatcggcg catcctgcac
     1201 gagatcatgg cgcccgaaga tgatggcacg gcgcagatcc ttgcgaaaga cgaacgcgtg
     1261 atccgttcgc tgaaagtact tctcgcgcag ctcttcggga acgccctcga cgtcgcgggg
     1321 cgagtagctc ccgaggcgcg cctgattgag cgcctcctcg tccgcgtcgg tcgcgtagat
     1381 cttcacccgc tcgagaaagc cggattcgcc gagtcgctcg gcaagaacca tcgcgacggt
     1441 gtaggcctct tcaccggacg cgcaggctgc gctccacacg cgaatcggcc ggtcgctctc
     1501 ggtcagcgcg ggagcgatgt gctcgcgaac gtagtcccac gcctgcgcat cgcggaagaa
     1561 cgacgtgacg ttgatcaaga tcgtgttgaa caggtgcata aactcttccg ggtgaacctg
     1621 aaggaagtcg gtgtaggcgg cgaactcaaa aacgccgacc gcgtccatgc ggcgtcgcac
     1681 ccggcggatc agactgctgc gcttgtaccc ggtgaagtcg aatcctcgcg tctgcttcag
     1741 gaagtgcagc agggcctcga atgcttcgtc ctggcctgca ccggcctcct cgctactcat
     1801 cagtcacctt ccggttgcgt tgcgggttcc ggcgagtcct ctgttgagac cccggagacg
     1861 gcgatcgcat cggcgactgc ggattgcagg accagccgct ggtcatcgat ttcctccgag
     1921 cgctcacgat accgcttcgc gagccacggc cgccgctcct cgttccgcac cgcgatccgc
     1981 cggaacaggt cgcgacgctc ctccatagag cggagcgcgg tccacagggc cgcctcgacc
     2041 tgctcgccgt tcgcggcaag cagggcttta tccgaaaaga catggcccac gcggcatcga
     2101 aagccgtttc ccgcctcggt ctcccagagc gtgccgccgc attccgggca cgtatgagcg
     2161 accggtaccg gccggggatc cgcctcgccg gtcataccgg tggccccctc cccggtgcgc
     2221 ggctcgcggg cccagcgggc gatcacgacg gggatctcgg cgagcggcac gtcgtagtcg
     2281 ggcgtgacga ggtgacgcgc gctttccacc atgtgcggaa agagcgcgtc cgactgcacg
     2341 accgtgacgc cgccggcccg gcgcacctcc gcgagtccgg tggcgccgtc gtcgagcgtt
     2401 ccggacagga tcacgcccat cacgttcggg ccccaccatt ccgcggccga gcgaagcgcc
     2461 ggatcgatcg cgggacgatg cccgttctcc ttggggccgg gcgtaaccaa aaggcgttcg
     2521 cccgagacca tcagatgccg gtccggcgga gcgacgtaga tgcggcccgg ccggatcgcc
     2581 tcgccgtccc gcggatgctc ggcgggaagc cggcccgccc gcgtgaggat cgcgggcatc
     2641 gcgcttcgga ccccgtgcga gacgtgcagc accatcagaa cggccgcgtc gagatcgtcc
     2701 ggcagaccgc tcgcgagccg tgcgagcgcg tccacaccgc ccgcggaggc gccgacgacc
     2761 acgaggccgc gcatacgatc gatcatacgt cctgcgactc ccaattgttt cggcgcgagc
     2821 gtgccgggcc cagtgtttcc ctctatgtca acaggctcct ccacggctcg gcccgagggc
     2881 cgcttccccg gctcacgggg cctacgccag caagacgccg ggattgagga tgtcgttggg
     2941 atcgaagacc ttcttgatgc cgcgcatcag ctcgatcttc gccgggtctt cgagctcgag
     3001 gaagtacttc ttcttggacc ggccgatccc gtgctcaccg ctgatcgcgc cgccgacgct
     3061 catgccggcc tcgaacagcg cgcgcatcac gcggtcacgc ttgccggggt cgggctggaa
     3121 caccgacaga tgcacgttgc cgtcgccggc gtggccgcag ccggcgagga acgtctcgtg
     3181 cttctcggcg atcgtgacga cgtgcgtgaa gaactcggcg atggacgcgc gcggcacgac
     3241 gatgtccacc agatccgcgg cgttggcggc cttggccgtc caaaacgcct tctcgcgcgc
     3301 ctcgatcagc tgctggcccg ccagcgggga aaggatgtag acatccatcg cgtccagccc
     3361 ggtgacgagc tcgccgacct cggcgacgtc ctcgtccagc cgatccggct tcgcggactc
     3421 gagcaccacc acaaggtagg cggtggcgga ttgcttgacg tcctccggga tgccgaggtc
     3481 caggcctacg tgcgcggtga tcgccgccat cgtgaaggcg tcgatgtatt cgaggatcat
     3541 cggcccggcg ccaccggcca cgacgcgcgg caccgcgcgc gtgacctcct cgagcgtgcg
     3601 gaagggcgcg agcacggtgg cgcggtgcgg caggcgcgga tgcagcttca ggatcgcttc
     3661 cgtcaccagc gcgagcgtgc cttcggagcc gatgatgagc tgggtcaggt cgtagccggt
     3721 cgaggacttc gcgagcttgc cgcccgtgcg gatgatgcgt ccgtcggcga gcacgcattg
     3781 caggccgacg atctggttgc gcgtcacgcc gtacttcacc gcgcgcatcc cgcccgcgtt
     3841 cgtcgccgcg ttgccgccga gcgagccgct cgtctcgccc ggatgcaccg ggtagaccag
     3901 cccgaacggg gcgagcgcct cgtcgagctg cgcgagcgtc acgccgggct gcacgacggc
     3961 gacgtggttc tcggtgtcga tctcgacgat cgcgttcatg cgctcgaatg agatcaggat
     4021 cgcatcggac ggcgggatcg cggcgcccga cagcccggtg ccgctgccac gcccgacgac
     4081 gggcgtgccg cgctcgttgg cgagcgcgca gatcgcggcg acctccccgg cggtaccggg
     4141 ccggacgacg accgcgggcg cggccgcctc ggccgagagc gtctcgtcgt gggtgtagtc
     4201 ctcggtgatc tcgtcaccgg tcaggacgtg ctctgcgggc aggatctgct gcagcgcttc
     4261 ggcgagatcg ctcatgaccc ggtcaagcct aaacgatgag ggcgggcacg cggcccgccc
     4321 tcatgcgtcg tggtggcgct acacgcacca gtcgaagccg aacaggctcg gcacgcgcgg
     4381 gatctgcgtt gtgcggtccg gccggcaggt taggtacggc tggccgttga acacgtcgat
     4441 gatctggccg gtgcggaaga agtgcgcccg ctgatcgccg ctgcgcggat cgcgtcgcgg
     4501 atcctcgtgc ggatcgcccc cgtcgagaat cgggggcacg tttccgttgg gctcgaggta
     4561 gttgccgctg tcccagatga tcagcgcact tccctcgtgc acggccggca tcgacggcat
     4621 gccgaacccg ggatcgacgc tgcggtgacg gcccggcatc agctgcgagc cgaggaacgc
     4681 ggcgccgatc gtacgcgcct cgacctccgc cgagacgggc gcgacctgga agtcgcccag
     4741 cgcgacgtgc agcagcacgc ggtgcgcggg cgtgttcggc agcggatcgt cggtcatgtg
     4801 ctgcgcatag ccgttcgcct cgccgcggtc ccagagcatc tgcagcacgg ccatcacgac
     4861 ctgctgctcc ttcttgtcgg ggaacatcgt gtacaggaac gacgagtacg agggcagacc
     4921 cggattgccc gggtcgacga gcggcccctc ccaatccacg gaacggttca gcagcgtcga
     4981 gtagttcatg ccgaccacgc cgagcgtggc gagggtgtga tccaccgcga gcgcggtgag
     5041 cgcgccgccc atgatgccgc cctggctgtt gccgtcgtag acgagcttgc cgggctcggc
     5101 gagccggttg ccggacgcgt ccttgaacgc ggggtcggcg gtcaggccgt ccgcgtggac
     5161 gagcgcacgg cccaggtaca ggaagttcac gaagccttgc tgcacccggt ccatcatcga
     5221 ggccatgttg gtcggatcgg tcagcgtcac gagcgcgttg gcgacgtcgt actccgcgaa
     5281 gccgaaccaa ttgaccgcgc acggcatgaa gttgcgctcg cgcatccgat ccgacgacga
     5341 gccggccgac tcgttgcggc tgccgagcag accatggccg tagagcatcg gatgcgcggg
     5401 attcgcggcc gtcgatgcgg ccgggatcgt gcacacgaac ggcgcctcga tcgttgggat
     5461 caccgggttc tgcatcggca gcccgtccgg gccgtacagg aaccgtccgg tggggatcgc
     5521 ctggttggga acgttgccgg caaccatggt gttgatgcgc tcggggccaa cgtgaaacgg
     5581 cgggcggtcg atgtagtacg gcaccgtgat cgttccctcg atgcgccgct ggcgcgttcc
     5641 cgagtcgccc gtgacactgg tgatcgagta cacaggtgcc gcgccctgaa cgatgccgtc
     5701 cgccaggttc gtgtcgccga gctgcgcgaa ggcgtcgtca cggatgtgca gcacccgctc
     5761 ggtcatcgac cgctgcgaga tgaccgtgaa gtcccacgca aggaacaggc tggtgggctc
     5821 gaacgcgtgg ttgccgcgcg cccactcggc agcggcgacc tcggcgacca cgccgcggat
     5881 gtgatcggca cggtcctgct cgacatcgac aggacgcgat cgctccatga tcgctgtgaa
     5941 ctcctcgccc cgttcgatgg gcaggccgtc ctcgttgcgc atgtttcgca acgcgaccag
     6001 atagcggcgg ccttcgagga agttcttcgc cggccgcagg atcagcagcc gctcgttgtc
     6061 ctgggtgaac tcgttggtgt cgagctcgga ccagaacggc cagcgctcgc cggtggtcgc
     6121 gtcgatgatc aggatcggcg cgtcgggcgc ctgatagcgg gcgatgtcgg cgatgtggtc
     6181 ggccggatcg ttcggtccgc cggtgctgat gccggcagtg ccccacgtct tgtgcaggtc
     6241 gaggccccgc acgaaggtca gaaccggcga acccggcgag aagccgtcgt tgcggttcca
     6301 ttcggtcggg tcgatcggct tcacgcccgc cccgcggggc atctcggcta cctggaagtc
     6361 cacgcgccgg cccgtgtcgg ttgacggatc ggccttggtg aagcggtcgt tcgggaacgg
     6421 cagcaggcag tgcgcccggt cgagcgcgtc acacggcgcg cgaagcgcgg ggggcgagac
     6481 ggcccccgcg ggcgcgacgg cggccacgcc ggccagcagc gacacggcca gcagaagtcg
     6541 aagggtcaag cgacgattcc cgggcacgac gacacctcgt ttcgatatag gcgggatatc
     6601 gggctatttc gccgcggcgg cccgatcctc ctgcccggcc gttgcgccgg ctgcccggag
     6661 cccggcccag cctccgaccg gctcaaccgg accgccgggg ctgctttttc cggaagttcg
     6721 cctcgagccg gtcgcaatcg cttgggacgg ggaacgccgg atggt
//
LOCUS       DATLFO010000242         4877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_114518, whole genome shotgun sequence.
ACCESSION   DATLFO010000242 DATLFO010000000
VERSION     DATLFO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114518"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VM841_09830"
     CDS             complement(<1..443)
                     /locus_tag="VM841_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVL90517.1"
                     /translation="MNDLRRPTFADVLTAARTIRPYLSKTAFHESVSLGKITGCRLFI
                     KYENHQPVNSFKIRGALNRMMATAPQERIRGFITSSMGNHGLGVCYAAKIIEAKATVC
                     VPEKANPDKVEAMRNLGAEVVAFGSDFEAASKSLPNATTSAPRLR"
     gene            complement(440..1462)
                     /locus_tag="VM841_09835"
     CDS             complement(440..1462)
                     /locus_tag="VM841_09835"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVL90518.1"
                     /translation="MSKRGHLAVVGAGSWGTAFATVLARNHLPTLLWARRAELARDIN
                     RSHRNEPYLPGIDLPTTLRATGDLEEAVGRASTIVIAVPSHAFRDKLKEMGPLIPADA
                     SVVHLTKGVEPETLLRMSEVIVEAGRVEPGRVGVVSGPNLAKEVARDLPGASVVACAE
                     PERARRLQRMFHCRTFRVYTNDDVCGVEIGGTTKNVIAIAAGIADGMGYGDNSKSALV
                     TRGLFEITRLGVRLGAQPMTFMGLAGIGDLVATCMSRQSRNRHVGEQLGLGRSLDEIV
                     AEMNMVAEGVKSCRAIMGLAERAGVDMPIVSRVARILFEGADPRAMVDDLIERAPEPE
                     FQGIGS"
     gene            complement(1459..2160)
                     /locus_tag="VM841_09840"
     CDS             complement(1459..2160)
                     /locus_tag="VM841_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVL90519.1"
                     /translation="MELAYGLVVWTIRPLFRVLFRWRMTGMEFIPQRGPAIFACNHIS
                     YLDPLAQGYVIVRRRIDRLRFFAKAELWKNPFLRLILKSAKQIPVERGSGEAGPVEGA
                     LNALRRGDAVVIYPEATITTTPDLSPMRGKTGVARVAVASGAPVIPVAIWGSHWVSPK
                     NRVRTRRLFRTIFVAAGEPMSFASLRGREDDPTTLRDVTDRIMGELERLVRDLQKDYP
                     GGAAVPPPLPGREAA"
     gene            2211..2843
                     /gene="cofC"
                     /locus_tag="VM841_09845"
     CDS             2211..2843
                     /gene="cofC"
                     /locus_tag="VM841_09845"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HVL90520.1"
                     /translation="MALVVVPAKPLSRAKLRLAPVLSPEERRFLCLAMLADVCAAAAE
                     AECGRVMVIASDTDAEAVARVFGATVVKDPTPEAGLNTSLDAAIPADPGGVLIVASDL
                     PACSAAELRSVAGFEGVRIATDAGGTGTNALWRRPPGVIAPAFGDDSARRHRKAAAEA
                     GAAFEAVAPAGLSFDVDLPTDLFLAWDAPVGPNTRSALRELGFPDRIITT"
     gene            2972..3178
                     /locus_tag="VM841_09850"
     CDS             2972..3178
                     /locus_tag="VM841_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HVL90521.1"
                     /translation="MAQGTIKSYDHETRSGVILDDDKNELFFDLDSFRGSGLRELRLG
                     QRVKYNVLGEAPRQKVRELQIVTM"
     gene            3296..4087
                     /locus_tag="VM841_09855"
     CDS             3296..4087
                     /locus_tag="VM841_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90522.1"
                     /translation="MAVFDRGGRGIAYRDEGDGPAVVLLHGLLMDGSLWDPQVEALRD
                     SYRVVTIEAPGHGDSDPAVDGYTFDDGAEGVWALLDGIGVERAVVGGQSMGGWTALRC
                     ALSRPHATRGLILIDTSAGPEDPEKLAQYEAFLDVALTDGVSDDLAAILLMLLFSGAF
                     AQTPAAEPWRKKLISSDPGRFAALARAVFSREPVEQRLGEIAAPALVIHGIEDISIPM
                     ERAEQLSTALRAPLHRIEGAGHAASHEKPAQVTPILRAFLDGLPG"
     gene            complement(4148..4663)
                     /locus_tag="VM841_09860"
     CDS             complement(4148..4663)
                     /locus_tag="VM841_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVL90523.1"
                     /translation="MRAIRRKQRFSRLAAQESIGSLDITGGGSLNRAQLLRTVAEKTE
                     MSKKDVEAVYENIIRSIQDAVKKGERVGLTGFGNFTQRVRKGRKAGMARNPFTGEMVK
                     VAARPAQKLPKFTPAKLFKEYVSGTVKSLPAVGGSKAAPKKAAAKKTTAAKKKTAAKK
                     PAKKTAAKKRR"
BASE COUNT          740 a         1699 c         1647 g          791 t
ORIGIN      
        1 atgcgcaacc tcggcgcgga ggtcgtcgcg ttcggcagcg atttcgaggc ggcctcgaaa
       61 tcgctgccga acgcgacgac ctccgcgccg aggttgcgca tcgcctcgac cttgtcgggg
      121 ttggccttct ccggcacgca gaccgtggcc ttcgcctcga tgatcttggc cgcatagcac
      181 acaccgagcc cgtggtttcc catcgacgag gttatgaacc cgcggatgcg ctcctgcggc
      241 gcggtcgcca tcatgcggtt gagcgcgccg cggatcttga aggagttcac cggctgatgg
      301 ttctcgtact tgatgaagag ccggcatccg gtgatcttgc cgagcgacac cgactcgtgg
      361 aacgcggtct tggaaaggta cggccggatc gtgcgagccg ccgtcagcac gtcggcgaag
      421 gtcgggcgcc ggagatcgtt cacgaaccga tcccttggaa ctcgggctcg ggtgcgcgtt
      481 cgatcagatc gtccaccatc gcacgaggat cggctccttc gaacaggatg cgcgcgaccc
      541 gcgacacgat cggcatatcc acgccggcgc gctcggccag acccatgatc gcgcggcacg
      601 acttgacccc ttcggcgacc atgttcatct cggccacgat ctcgtccaga gatcgtccga
      661 ggccgagttg ctctccgacg tgccggttgc ggctctgccg gctcatgcac gtcgccacca
      721 gatcgccgat cccggcgagt cccatgaacg tcatcggttg cgcgccgagg cggacaccga
      781 gccgggtgat ctcgaacagg ccgcgcgtga cgagcgcgct cttggagttg tccccgtagc
      841 ccatcccgtc agcgatgccc gcggcgatcg cgatcacgtt cttggtggtt cccccgatct
      901 cgaccccgca cacgtcgtcg ttggtataga cccggaaggt ccggcaatgg aacatgcgct
      961 gcagccgccg cgcgcgttcg ggctcggcgc acgccaccac gctcgcgccc ggaaggtccc
     1021 gcgccacctc cttggccagg ttcgggcccg acacgacgcc gacccggccg ggctcgaccc
     1081 ggccggcctc gacgatgacc tcgctcatgc gcagaagcgt ctcgggctcg acgcccttgg
     1141 tgagatgcac cacgctcgcg tccgccggga tcagcggccc catctccttg agcttgtcgc
     1201 ggaaggcgtg gctgggcacg gcgatcacga tggtcgaggc gcgtccgacc gcctcctcga
     1261 ggtcgccggt cgcgcgcagg gtcgtgggaa ggtcgatgcc cggcagatag ggctcgttgc
     1321 gatggctgcg attgatgtcg cgtgccagct ccgcccgccg ggcccacagg agcgtcggca
     1381 gatggttgcg cgccaggacg gtcgcaaagg ccgttcccca ggagcccgcg ccgaccacgg
     1441 cgagatggcc gcgcttgctc atgcggcctc ccgtcccggc agcggcggtg gaaccgctgc
     1501 cccgccggga tagtccttct gcagatcccg caccagccgc tcgagctcgc ccatgatccg
     1561 gtcggtgacg tcgcgcagcg tcgtgggatc gtcttcgcgg ccgcgcaggc tcgcgaacga
     1621 catcggctcg ccggctgcga cgaagatggt gcggaacagc cggcgcgtgc ggacgcggtt
     1681 cttcggcgac acccagtgcg atccccatat ggcgaccggg atcacgggcg cgccggatgc
     1741 gacggccaca cgcgccacgc cggtcttgcc gcgcatcggg gacaggtccg gggtcgtggt
     1801 gatggtcgcc tcggggtaga tcacaacggc atcgccccgg cgcagcgcgt tcagcgcgcc
     1861 ctcgaccggg ccggcctcgc cggagccgcg ctcgacgggg atctgcttcg cgctcttgag
     1921 gatcagccgc aggaacgggt tcttccacag ctcggccttg gcgaagaagc ggagacgatc
     1981 gatgcgacgg cgcacgatga cgtagccctg ggcgagcgga tccaggtacg agatgtggtt
     2041 gcacgcgaag atcgccggcc cgcgctgcgg aatgaactcc atccccgtca tgcgccagcg
     2101 aaagagcacg cgaaacagcg gacgaatcgt ccagaccacc agcccgtacg cgagctccaa
     2161 actcgattac ctccggatcg tcgtccccgc ctattctagc cgcgaccctc atggccctcg
     2221 tcgtggtgcc cgccaagccg ctctcccgag cgaagctccg gctcgcgccg gtgctgtcgc
     2281 ccgaggagcg gcggttcctg tgcctggcga tgctcgccga cgtctgcgcc gcggcggccg
     2341 aggccgagtg cggccgggtc atggtgatcg cgagcgacac cgacgccgag gccgtggcgc
     2401 gcgtgttcgg ggcgacggta gtgaaggatc cgacgcccga agccggtctg aacacctcgc
     2461 tggacgccgc gatcccggcg gatccgggcg gggtgctgat cgtcgccagc gacctgcccg
     2521 cctgctcggc cgcggagctt cgctcggtcg ccggattcga gggcgtgcgg atcgcaaccg
     2581 acgcgggcgg caccggcacc aacgcgctgt ggcgccggcc gcccggcgtg atcgcgcccg
     2641 ccttcggcga cgacagcgcc cggcgccacc gaaaagccgc cgccgaggcg ggcgccgcgt
     2701 tcgaagccgt cgctcctgcc gggctgtcct tcgacgtgga cctgcccacc gaccttttct
     2761 tggcatggga tgccccggtc ggtccgaaca cccgctcggc gcttcgcgag ctggggttcc
     2821 ccgatcggat catcaccacc tgaccgccgc ctgaccgccg cctgaccgcc gcctgaccgg
     2881 cacgccggtt gtcgccctcc acggcccgtc gggtatcgtt tgcggcgcac cgatgcggca
     2941 acgcgaaggc aggcgaaagg aaactcgggc aatggcgcaa ggaaccatca agtcctacga
     3001 ccacgagacc cgctcgggcg tgatcttgga cgacgacaag aacgagctgt tcttcgacct
     3061 ggactcgttt cgcggcagcg gcctgcgcga gcttcgcctg ggccagcggg tgaagtacaa
     3121 cgtcctcggc gaggcacctc gccagaaggt tcgcgagctc cagatcgtca ccatgtagcc
     3181 gaggggtagc ccggctggca gtccgcgggc gtccacggga aagtccacga gaagtccacg
     3241 ggcagccggc agggagccgg ggcgggcgga cggtccgccg agggaggatg agggcatggc
     3301 ggtcttcgac cggggcggac gaggcatcgc gtatcgcgac gagggcgatg gacccgcggt
     3361 cgtgctgctg cacggcctgc tgatggacgg atccctctgg gatcctcagg tcgaggcgct
     3421 tcgcgactcc tatcgcgtcg tcacgatcga ggcgcccggc cacggcgaca gcgatccggc
     3481 cgtcgacgga tacaccttcg acgacggcgc ggaaggcgtg tgggcgctgc tcgacggcat
     3541 cggtgtcgag cgcgcggtgg tcggcggaca gtcgatgggc ggctggaccg cgcttcgctg
     3601 tgcgctgtcc cggccgcacg cgacccgggg cctgatcttg atcgacacct ccgccggtcc
     3661 cgaggacccg gagaagctcg cacagtacga ggcgttcctg gacgtcgcgc tgaccgacgg
     3721 cgtctcggac gatctcgcgg ccatcctgct gatgctgctg ttctcgggcg cgttcgcaca
     3781 gacgccggcc gcagagccgt ggcgaaagaa gctgatctcg tccgacccgg gccggttcgc
     3841 ggccctggcc cgggcggtgt tctcccgaga gccggtcgag cagcgcctgg gcgagatcgc
     3901 cgcacctgcc ctcgtcatcc acggcatcga agacatctcc atccccatgg agcgcgcgga
     3961 gcagctctcg acggcgttgc gcgcgccgct gcatcggatc gagggcgccg gacatgcggc
     4021 ctcacacgag aagcctgcac aggtcacccc cattcttcgc gcgttcttgg acggcctgcc
     4081 cgggtgaggg cggttgaaac gcgaatgagg gcgggatttc ccgccctcat tcgtttccgg
     4141 cttcttctta gcgccgcttc ttggccgccg tcttcttggc aggcttcttg gcggcggtct
     4201 tcttcttggc ggcggtggtc ttcttggccg cggccttctt cggggcggcc ttcgagccac
     4261 ccacggccgg cagcgacttg accgtaccgc tcacgtactc cttgaacagc ttcgccggcg
     4321 tgaacttcgg gagcttctga gccgggcgcg cagcgacctt caccatctca ccggtgaacg
     4381 ggttgcgggc catgccggcc ttgcgtccct tgcggacacg ctgcgtgaag ttgccgaatc
     4441 cggtgagccc gacgcgctcg cccttcttca cggcgtcctg gatcgaccga atgatgttct
     4501 catagacggc ctcgacgtcc ttcttggaca tctccgtttt ctcggcgacc gtccggagca
     4561 actgcgccct attcaacgaa ccacctcccg tgatgtcgag actcccgatg ctttcttggg
     4621 ccgcgagcct cgaaaaccgc tgtttcctgc gaatcgcgcg caacgataga ggacgagcgg
     4681 tcgagagtca aagaagatgg gagcaaaaaa tccccgaaat cttaggggtt ttcgacccct
     4741 cgcagaccta gaagaagtcc ggcgtccagg gcgcggggcc cgcaaagatc tccgccaggc
     4801 gctcgagcga gcccgccgcg ccgtccagca agcccgaccg gcgcgcatcg tgcggcgtga
     4861 tcaggccgct atagatg
//
LOCUS       DATLFO010000243        14039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_14704, whole genome shotgun sequence.
ACCESSION   DATLFO010000243 DATLFO010000000
VERSION     DATLFO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14704"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VM841_09865"
     CDS             <1..707
                     /locus_tag="VM841_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90524.1"
                     /translation="GSSVDVRYALTGPAGDTMTTYAERRAGAGEDVLRGLVAGVASGL
                     IDNSGRQFGIFGVRYVFVRGDADPALRDAFERQSDLRFVQRFNRTAVFVNDLWIPVGV
                     GIGSQNWTVAANAERAPEAIAGTTGSPEPGDGFVRLRPDVFTGTVREGAKRVLLATAF
                     DDRWRLVLLDADRGVFAEHAWGWANGFPAPAAGRAALVWHGQARYRLLLLGQLVILIA
                     AAALWSRRVAAERGER"
     gene            704..2128
                     /locus_tag="VM841_09870"
     CDS             704..2128
                     /locus_tag="VM841_09870"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HVL90525.1"
                     /translation="MKVPSRLAVGLAFALLLGGAIAADRMEPPSPIAAPPVVPLTPGG
                     VLACPFLFNGNGSSWLHLVNAGSDTSKVTVTTVRENADPLFRTFTLDAGKTTTMTLDP
                     AVRQASGAIVQFSGGEVAASRASMFGAGAGGRAGAAASCARAGDRTIVVPSGATLGAD
                     TTVLLLNPSTSDAVADVSLLTGGEELRPEGLKGIVVEARSLRRIRVGDFAFDERFVST
                     VIRVRTGVLAADGLISAPSGISLVPGGPPRRDLAGVATLSAGQGLIDVVGVGEADTVA
                     DAALLSPDGLTAFTALSEAIVPSTPQTVSLLPEGERIAATGAVLGVREGSGLSAALRW
                     VVRLPNGRGDVSASEMLPPARRLMAVTGAPASAAQSRILLGNPGNAPAIVNLDLLTEQ
                     GRQSPAPFQGIRIEPGQALSLEIGTFPGSFAVVAVADRPVVMAISSTALSPVLASFTV
                     TGHAAPAPQAVPVEPDPRVGVPAG"
     gene            complement(2125..2475)
                     /locus_tag="VM841_09875"
     CDS             complement(2125..2475)
                     /locus_tag="VM841_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HVL90526.1"
                     /translation="MRKQVDARERFERYVQDALDSLPEELASQMDNVAIVVEDGTADG
                     PLLGLYHGIDQTARDSWYAGVLPDVITIYRLPLEARARSEADLAEQVRVTVWHEIAHH
                     FGISDERLHELGWD"
     gene            2906..3199
                     /locus_tag="VM841_09880"
     CDS             2906..3199
                     /locus_tag="VM841_09880"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HVL90527.1"
                     /translation="MARGGGTEVRRCSRNGCRWPASASLTFAYAQRVVWIEDLTGLPH
                     PAVYDLCAAHAERLAVPIGWTKEDLRQVPPPVTPIRPDVDVWGERRDASASGA"
     gene            3405..3704
                     /locus_tag="VM841_09885"
     CDS             3405..3704
                     /locus_tag="VM841_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90528.1"
                     /translation="MPIDRDLIRRIRGLDEYDLRRLMIFVRGLLIGSEGGPAFEPPGR
                     AVEPKVTYRQQSVRCGKNGCTRCPHGPYWYAYWREEGRLRSRYIGRTLPEALGQD"
     gene            3855..4055
                     /locus_tag="VM841_09890"
     CDS             3855..4055
                     /locus_tag="VM841_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90529.1"
                     /translation="MSGLMCPTCASRDVININLTLERGDRVAFYSCHRCDKKWWGKDG
                     EAVSLGNVLEMARRVPQARKVG"
     gene            4182..4901
                     /locus_tag="VM841_09895"
     CDS             4182..4901
                     /locus_tag="VM841_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90530.1"
                     /translation="MPSIERAVTLAFAGKLREAERIAGESSSPEASWLRAYLHNCFGR
                     FDQALELAMDLAQGSADSDLAARSLITAASSLRQTGRHLPAHPLDESAMRLATTDATR
                     AHALIGLAADAAGLGRESSEPIEQAAALAPPGDWRVRVRLAWVRCEQALVAGRARRAA
                     TNARDALDRSRAAGARRHEAKSLLFLAFSLREAGDHDWVADMKLAKSIAARIGAAPVA
                     AVAAEALDAPRDGASPASQGR"
     gene            4919..6247
                     /locus_tag="VM841_09900"
     CDS             4919..6247
                     /locus_tag="VM841_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HVL90531.1"
                     /translation="MDRIFKAYDVRGVYPDEIDQEVAHRTGRAFVDFLGARKVIVGRD
                     ARASSEPLSTAFIAGAVAQGADVVDIGLASTDMLYFASGVLDAPGAMFTASHNPPKYN
                     GIKMCRAAAAPIGENSGLLDIKEMVRANEAAPVRRTGRIEQRDMLDDYVKHVLSFVDV
                     SKLRPLVVVADAANGMGGLTVPSVFAQLPFELIPLYLEIDGTFPNHPADPLQPENLRD
                     LQDAVLRNGAAVGLAFDGDADRCFIVDEKAQPVSGSLTAALVARSILRKNPGETVIHN
                     IICSRVVPETIREHGGTPVRTRVGHSFVKKVMAETGAIFGGEHSGHYYFRDFWRADSG
                     LVAALHVLEAISEENAPLSEVLAALQRYWNSGEVNIEVSDQQGAMERVAASFADATPD
                     WSDGLTVEFADWWFNLRPSNTEPLLRLNVEARDAAGGETRRDEILSIVNG"
     gene            6265..6429
                     /locus_tag="VM841_09905"
     CDS             6265..6429
                     /locus_tag="VM841_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HVL90532.1"
                     /translation="MDQVLLDILVCPNCKGQIEYVEAEEKIYCRGDCKYVYPVRDGIP
                     IMLIDEAEKP"
     gene            6432..7478
                     /locus_tag="VM841_09910"
     CDS             6432..7478
                     /locus_tag="VM841_09910"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HVL90533.1"
                     /translation="MLDDDALYERVDPSGMLAAVEGWPDQWEAACRATERIASIEGRF
                     ESAVVCGMGGSGIAGDVVRGLLDPALPIPIRLVRGHEIPGFVGERTFVAALSYSGNTS
                     ETLACFDAAVERGATIVAITAGGALAERARAAGAVLYSDLPAGIMPRAALPSLVVPLA
                     VAIGRAGVADTSGLLEMGGQVVRRSVEAWRREIPAERNLAKRIATAIGDRFPIVWGSE
                     GTQTVAAMRWKCQLAENAKLPSIMSVLPELGHNDIVGVHAGHDALRDAILLALRVEGG
                     HPAQEARLTAALDIAGRSVGAVEVVTIESSSPAERLIESLILADFVSVYNAVLRGVDP
                     SPIEAIDLLKAAQA"
     gene            7548..8804
                     /gene="ahcY"
                     /locus_tag="VM841_09915"
     CDS             7548..8804
                     /gene="ahcY"
                     /locus_tag="VM841_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVL90534.1"
                     /translation="MNGHIADPGLAEKGRIRIDWADQQMPVLREIRARFAKEKPLAGL
                     KIAACLHVTTETANLMRALVAGGADAVLCASNPLSTQDDVAAALDADGIPTYAIKGED
                     NETYYSHIESVIAFGPQITMDDGADVVSILHSKHPDKAAAVLAGTEETTTGVIRLRQM
                     AAENVLMYPIVAVNDTPTKHMFDNRYGTGQSTLDGITRATNILWASRTVVVCGYGFGG
                     KGLSMRARGLGAQVIVTEVDPVKALEAVMDGYRVMPIADAAREGDVFITVTGDRDVLR
                     GEHFEMMKDGAILANTGHFDVEIDKNALASLATDEPKKIRDFVEEFRMSDGRRLYLLA
                     QGRLVNLAAAEGHPATVMDMSFADQALTAEWLAKGQVPLEKRVYDVPREIDEEVARLK
                     LQTLGLRIDELTPEQVKYLSSWREGT"
     gene            9027..9527
                     /locus_tag="VM841_09920"
     CDS             9027..9527
                     /locus_tag="VM841_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90535.1"
                     /translation="MSRKTTRRTARRLLGVAVLAGLSILTSTTARAANASATSLAASK
                     VKGDSAQFPVFGVTLTQPAEVDSLTDVVVRFQSMVNFSTGDLAPDFSAFGCTNGDATS
                     CDGVPCASSRWERAVRPHVARASPPTGTRWASEASSRKQAAAPRQGLGAAARVPAPGR
                     DGPVRA"
     gene            9591..10922
                     /locus_tag="VM841_09925"
     CDS             9591..10922
                     /locus_tag="VM841_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90536.1"
                     /translation="MRRASLRAFALALAAVTAAALAASPAATAAAFGSRAGGKLIAPG
                     RTAPAAVTDGAGGVIVSSRDATDGVAVVNRYDALGSPVWDQGIEFPDASGIQLAADGS
                     GGAIVAVHHPDSAVMTVGRILHSGATTWQARFPSGALVAGGDGGAWIVSQSGEIFADG
                     VDAAGALRPRLQVTTSPDAESLPVAIGDGAGGAYLAWRAALSETQFALRLQHLTAAGA
                     LWAAPAEVAGSSDSSITPRLAPDGRGGVVVSWDAAGLVWVHDYSSDGIPVWDQVVTIP
                     ATEGTVSAVGASGGEVFVAWTRRLRTLPSPTDVLNVSFVASTGAMHWTRHVAVVDNGV
                     RGLTIDAAVGAAVVAWEHAAIPFAGAALDPDLSIYRVTSGGEETYPAHRRPLADSFGP
                     ESLGALVAAGGGSVVAVFTQTPCFRGDVSGVAMQRIAAGGERSFRADGACP"
     gene            10976..11500
                     /locus_tag="VM841_09930"
     CDS             10976..11500
                     /locus_tag="VM841_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90537.1"
                     /translation="MPDEPASSYPGVPAPSQNGHRRPSRAPVRDERPLRGVVAGTLAG
                     AIAAAIAIPRVLLLNLALFNPWPRPVIAAALAGLVTGVMRRGGRRRGAMIAGAAAASL
                     GLLVAYAGVRFSVRVLWVDRNPAAVIAADVFRLTAYAIVPGALAGAAGWLLRGAGRRA
                     RNRRAIAKPPIVRL"
     gene            11521..12597
                     /gene="mtnA"
                     /locus_tag="VM841_09935"
     CDS             11521..12597
                     /gene="mtnA"
                     /locus_tag="VM841_09935"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HVL90538.1"
                     /translation="MTEPAASPMADALRYDEGAIYAIDQTALPAKTVVRKITTTGQLA
                     EAILKLRIRGAPMLGIAGAWGVAQAARRAGSKRGATRNAVMAAAQRAGERLAATRPTA
                     VNLGWAIERSLGRARLEAAAGAPPATIVKALEEEAAAIASEDAAACMAMAETGQRFVR
                     GGARIMTHCNTGSLCTGGYGTALGVIRLAHESGKDIHVLAGETRPLLQGARLTAWELG
                     RLQIPHAIVPDGAGPALIARGEVDLVLVGADRIAANGDVANKIGTYSLALAATESQVP
                     FIVVAPMSTIDPSTPDGAAVEVEERDGREVVSVLGRRVAPPGSGARNPAFDVTPSRLI
                     SAIVTERGVAMRPYRTSLKRLLAQ"
     gene            12664..13320
                     /locus_tag="VM841_09940"
     CDS             12664..13320
                     /locus_tag="VM841_09940"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HVL90539.1"
                     /translation="MPLLDLLYPRVCAACDAHGRAPLCAACDAGAPWIGDACLRCALP
                     LCRGCAGRGLAFDRAASAAIYAGAARDALMRFKAAGEHRVAAALAARMTGAARRAGIG
                     AAGDTVCTWVPSGRAALRARGRNPAELLARPLARSLAVAARPLLVKTRETPDLAGLAR
                     AARRRALEGVFAIAAPVPPRVLLVDDVLTTGTTASACAGALKAGGASRVDVVTFARAL
                     "
     gene            13704..>14039
                     /gene="raiA"
                     /locus_tag="VM841_09945"
     CDS             13704..>14039
                     /gene="raiA"
                     /locus_tag="VM841_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVL90540.1"
                     /translation="MQVIVKARHTRVPAALKKMATEKVEKVRRFFDRIDKIEIEFSEE
                     HNPRVANKHVVEVTLSAKNHVLRAVGKGADPASAVDQVVDKIERQVRRMKDKMVKRGR
                     ASAKTETIRK"
BASE COUNT         1991 a         4946 c         4976 g         2126 t
ORIGIN      
        1 gcggaagcag cgtcgacgtg cgctacgcgc tcaccggccc ggccggagac accatgacga
       61 cctacgccga gcgccgtgcc ggggccggcg aggacgtgct ccgcgggctg gtcgcgggcg
      121 tggcctccgg gctcatcgac aacagcggcc gtcagttcgg gatcttcggc gtgcgatacg
      181 tgttcgtgcg cggtgacgca gatccggcgc tgcgcgacgc cttcgagcgg cagagcgatc
      241 tgcgcttcgt gcagcgcttc aaccggaccg ccgtcttcgt caacgacctg tggatcccgg
      301 tcggcgtcgg catcggatcg cagaactgga ccgtggcggc caacgccgag cgcgcacccg
      361 aggcgatcgc cggaaccacc ggcagcccgg agccgggaga cgggttcgtc cggctgcggc
      421 ccgacgtgtt cacgggcacg gtgcgcgagg gagccaaacg cgtcctgctg gccaccgcct
      481 tcgacgatcg gtggcgcctg gtcctgctgg acgcggaccg cggggtcttc gccgagcatg
      541 cctggggatg ggcgaacggg tttccggccc cggcggccgg ccgggcggcg ctggtgtggc
      601 acggccaggc gcgctaccgg ttgctgctgc tcggccagct cgtcatcttg atcgccgcgg
      661 cggccctgtg gtcgcgccgc gtcgccgccg agcgaggtga acgatgaagg tgccctcgcg
      721 gctcgcggtg gggctggcgt tcgcgctgct gctcggcggc gccatcgccg ccgaccggat
      781 ggagccgccc tcgccgatcg ccgcgccgcc ggtggtgccg ctcacgcccg gaggcgtgct
      841 ggcgtgcccg ttcctgttca acggcaacgg aagctcctgg ctccatctcg tcaacgccgg
      901 cagcgatacc tccaaggtca ccgtcacgac cgttcgcgag aacgccgatc cgctgttccg
      961 aaccttcacg ctcgacgccg gaaagaccac cacgatgacc ctggatcccg ccgtccggca
     1021 ggcgtccggc gcgatcgttc agttctccgg cggcgaggtg gccgcgtccc gcgcgtcgat
     1081 gttcggcgcc ggagccggcg ggcgggccgg cgccgccgca tcctgcgccc gcgcgggcga
     1141 ccgcaccatc gtggttccca gcggagcgac gctcggcgcc gacaccaccg tgctgctgct
     1201 caacccgtcc acgtccgacg ccgtcgccga cgtgagcctg ctcaccggcg gcgaggagct
     1261 gcggccggag ggcctgaaag gcatcgtcgt cgaggcccgg agcttgcggc ggattcgcgt
     1321 cggcgacttc gcgttcgacg agcgcttcgt ctcgacggtg atccgggtac gcacgggcgt
     1381 gctcgccgcc gacggcttga tctcggctcc ctcgggcatc tcgctcgtcc cgggcggtcc
     1441 gccccgccgg gaccttgcgg gcgtggcgac cctgagcgcc gggcaagggc tgatcgacgt
     1501 ggtcggcgtg ggcgaggccg ataccgtcgc cgatgcggcg ctgctgtcac ccgacgggct
     1561 gaccgcgttc accgcgctgt cggaggcgat cgtgccgagc acgccccaga cggtctcgct
     1621 gctgcccgag ggcgagcgga tcgccgcgac cggtgcggtg ctcggcgtgc gcgaagggtc
     1681 gggtctgtcg gcggccttgc gctgggtcgt gcgccttccc aacggccggg gcgatgtgtc
     1741 cgcctcggag atgctcccgc cggcccgccg cttgatggcc gtgaccggtg cccccgcctc
     1801 ggccgcgcag tcgcgcatcc tgctcggcaa tcccggcaac gcgcccgcga tcgtgaacct
     1861 ggacctgctc accgagcaag gacggcagtc gccggctccg ttccagggca tccggatcga
     1921 gccggggcag gcgctctcgc tcgagatcgg cacgttcccg ggatccttcg ccgtggtcgc
     1981 ggtcgccgac cggcccgtcg tgatggcgat ctcctccacg gcgctttcgc cggtcctggc
     2041 ctcgttcacc gttaccggcc acgcggcgcc tgcgccgcag gccgtgccgg tcgagcccga
     2101 cccgcgggtc ggcgtgccgg ccggctagtc ccagccgagc tcgtggagcc gttcgtcgga
     2161 gattccgaag tggtgggcga tctcgtgcca caccgtcaca cgcacctgct cggccagatc
     2221 cgcctcgctg cgggcgcggg cctcgagcgg caggcggtag atggtgatga cgtcggggag
     2281 cacgccggcg taccaggagt cgcgcgcggt ctgatcgatg ccgtggtaga ggccgagcaa
     2341 cggaccgtcc gcggtgccgt cttcgacgac gatcgcgacg ttgtccatct gcgaggcaag
     2401 ctcttcggga agcgagtcga gcgcgtcctg cacgtagcgc tcgaaccgtt cgcgggcatc
     2461 cacctgcttg cgcacgcggt cgccccagga atctccggtc gatcggcgag aaattttcgg
     2521 cttccccctt gaaagtgtcc gtagggtgtg ataggttagc cgagtactcc gcagaagtca
     2581 gtaggggcaa cccgagagct actgcggggt acaactttta ttttcaagac cgtcggcggg
     2641 ccttccaggg cccgctttgt gcataccgga aaggccgcct gacctgcggc ctttttctat
     2701 tggggccgga gggaccgccg ggcggcggac cgggccggcg ggtctccggc agccgagcga
     2761 aaaatagcgg gcgctcggca aacgccaaaa gtatcttatg tagcggagaa agaaggtaag
     2821 aagcactcat gcccgcgacg ccgggaccat cgaaacgccc tgcaaccttg aaaaagacac
     2881 gggtgtaatt tactgttcgc cttcgatggc acggggcgga ggaactgaag tcaggcgctg
     2941 ttcgcgcaac ggctgccgct ggcctgcctc ggcctcgctc acattcgcct acgcccagcg
     3001 cgtggtgtgg atcgaagatc tcaccggact gccgcatccg gccgtctacg acctgtgcgc
     3061 cgcgcacgcc gaacgccttg cggtgccgat cggctggacg aaggaagacc tgcggcaggt
     3121 gccgccgccg gtgacgccca tccgtcccga cgtggacgtt tggggcgagc ggcgcgacgc
     3181 atccgcgtcg ggcgcctagc ggctccggcg gcgccgctcc tcgcagctgc cccaccccgc
     3241 tccgcactcg atcccgcggc tctcggccgg cccgcccgac cccgatgcgc cgccgatgaa
     3301 tctttttttc gggccgccgg cgactctgcg gaaagtcttt gcttctcaac gcttgactgt
     3361 tacgatcggc ctgctcttag gtacgacacg aggtaagact tttcatgccg atcgatcgcg
     3421 atctgatccg acgcatcagg ggcctggacg agtacgacct tcggcggctg atgatcttcg
     3481 tccgcggcct gctgatcggg tcggagggcg ggccggcgtt cgagcccccg ggccgggcgg
     3541 tcgaaccgaa ggtgacctac cgccagcaga gcgtgcgctg cggcaagaac ggatgcaccc
     3601 gctgtcccca cggcccctac tggtacgcct actggcgcga agaggggcgt ctgcgctctc
     3661 gctatatcgg acgcacgctg cccgaggcgc tcggtcagga ctgaacgaac ccctataaac
     3721 caggacgaaa tccacccgca aagggtgcga cgacgaaaaa aaccgctccg acgggctatg
     3781 gagatcgtcc tgatccgccg agatacttct cgccggaacc tacggtgata gcggcctcgg
     3841 ttatgggggg tttcatgagc ggcctgatgt gtcctacgtg cgcttcgaga gacgtgatca
     3901 acatcaacct cactctcgaa cgcggcgatc gtgttgcgtt ctactcgtgc caccgctgcg
     3961 acaagaagtg gtggggcaag gatggggaag cggtcagcct cggcaacgtc ctcgagatgg
     4021 cccgacgcgt tccgcaggct cgcaaggtcg gttagcgagc actgacttcc ccggtgcccg
     4081 gcccgcaaag gccgggcacg cgcatgtccg gaccgtcccg cgcatgtccg gaccgtcccg
     4141 cgcgtgcgcc gcgatcaggt cgggcggatt attctcggcc cgtgccctcc atcgagcgtg
     4201 ccgtcaccct cgcctttgcg ggaaagctgc gcgaggccga gcgcatcgcc ggcgagtcct
     4261 cctccccgga ggcgtcgtgg ctccgggcgt acctgcacaa ctgcttcggg cggttcgatc
     4321 aggcgctcga gctggcgatg gacctggccc aaggcagcgc cgactccgac ctggcggcgc
     4381 gctcgctcat caccgccgcc tcgtcgctgc gtcagacggg ccggcatctt cccgcgcatc
     4441 cgctcgacga gtcggcgatg cgccttgcca cgaccgacgc cacgcgcgca cacgccctga
     4501 tcgggctcgc cgcggacgcg gcggggctcg gccgcgagtc ctcggaaccg atcgagcagg
     4561 ccgcggcgct cgcgccgccg ggcgactggc gcgtgcgcgt ccgcctcgcg tgggttcgtt
     4621 gcgagcaggc gctggtcgcc ggccgagccc gcagggcggc tacgaacgcg cgcgacgcac
     4681 tggaccgctc ccgcgccgcc ggcgctcgcc gccacgaggc caagtcgctg ctgtttctgg
     4741 ccttctcgct gcgcgaggcc ggcgaccacg attgggtcgc cgacatgaag ctcgccaagt
     4801 ccatcgccgc ccggatcggg gccgcgccgg tggcggccgt cgccgccgag gcgctcgatg
     4861 cgcctcgaga cggcgcatcg cctgcctcgc aaggccgata ggattcccgc tcgtggacat
     4921 cgaccggatc ttcaaggcat acgacgtccg gggcgtgtac ccggacgaga tcgaccagga
     4981 ggtcgcgcac cggaccggcc gcgcctttgt ggacttcctg ggcgcgcgca aggtgatcgt
     5041 cgggcgggac gcgcgcgcct cgtccgagcc gctctcgacc gcgttcatcg ccggcgcggt
     5101 cgcgcaaggc gccgacgtcg tggacatcgg tctggcctcg accgacatgc tctacttcgc
     5161 ctccggtgtg ctcgacgcgc cgggtgcgat gttcaccgcc tcgcacaacc cgcccaagta
     5221 caacggcatc aagatgtgcc gcgcggcggc cgcgccgatc ggcgagaact ccggcctgct
     5281 cgacatcaag gagatggtgc gcgccaacga ggcggcgccc gtgcgccgga ccggccgcat
     5341 cgagcagcgc gacatgctcg acgactacgt caagcacgtg ctgtcgttcg tggacgtgtc
     5401 caagctgcgg ccgctcgtcg tcgtggccga cgcggcgaac ggcatgggcg gcctgaccgt
     5461 gccgagcgtg ttcgcgcagc tgcccttcga gctgatcccg ctctacctcg agatcgacgg
     5521 cacctttccc aaccatccgg ccgatccgct gcagcccgaa aacctgcgcg acctgcagga
     5581 cgccgtgctg cgcaacggcg ccgccgtcgg gctcgcattc gacggcgacg ccgatcgctg
     5641 cttcatcgtc gacgagaagg cgcagccggt ctcgggatcg ctcaccgcgg cgctggtcgc
     5701 gcgctcgatc cttcgcaaga accccggcga gaccgtgatc cacaacatca tctgctcgcg
     5761 cgtggtgccc gagacgatcc gtgagcacgg cggcaccccg gtccgcacac gcgtgggcca
     5821 ctcgttcgtc aagaaggtca tggccgagac cggcgcgatc ttcggcggcg agcactccgg
     5881 ccactactac ttccgcgact tctggcgggc cgactccggg ttggtcgccg cgctgcacgt
     5941 gctcgaggcg atcagcgagg agaacgcgcc gctgtcggag gtgctcgccg cgctgcagcg
     6001 ctactggaac tcgggcgagg tcaacatcga ggtgagcgac cagcagggcg cgatggagcg
     6061 ggtcgccgcc tcctttgcgg atgcgacgcc ggactggtcc gacgggctga ccgtggagtt
     6121 cgccgattgg tggttcaacc tccggccgtc caacaccgag ccgctgctgc ggctcaacgt
     6181 tgaagcgcgc gatgcggccg ggggcgagac gcggcgcgac gagattcttt caatcgtgaa
     6241 cggataggag gaacggcgat gccggtggac caggtgctgc tcgacatctt ggtctgtccc
     6301 aactgcaagg ggcagatcga atacgtcgag gcggaagaga agatctactg tcgcggggac
     6361 tgcaagtacg tgtatccggt gcgcgacggc attccgatca tgctcatcga cgaagcggag
     6421 aagccctgag cgtgctcgac gacgatgcgc tctacgagcg cgtcgacccc tccggcatgc
     6481 tcgccgcggt cgagggctgg ccggaccaat gggaggcggc ctgccgcgcg accgagcgca
     6541 tcgcctcgat cgaaggacgc ttcgagtcgg ccgtggtgtg cggcatgggg ggcagcggca
     6601 tcgccggtga cgtggtgcgc gggctgctcg acccggcgct gccgatcccg atccgtctcg
     6661 tgcgtggcca cgagatcccc ggcttcgtcg gcgagcgcac cttcgtggcg gccttgtcct
     6721 acagcggcaa cacctccgag acgctcgcgt gcttcgacgc cgcggtggag cggggcgcga
     6781 ccatcgtggc gatcaccgcc ggcggcgcgc tggccgagcg agcgcgcgcg gccggcgcgg
     6841 tgctgtactc ggacctgccc gccgggatca tgccgcgcgc cgcgctgccc tcgctcgtgg
     6901 tgccgctcgc ggtcgcgatc ggccgcgccg gcgtcgccga caccagcggg ttgctcgaga
     6961 tgggcgggca ggtggtccgc cggagcgtcg aagcgtggcg acgcgagatc ccggcggagc
     7021 gcaacctcgc caagcggatc gccacggcga tcggcgaccg ctttccgatc gtgtggggga
     7081 gcgagggcac acagacggtc gcggcgatgc gatggaagtg ccagctggcc gagaacgcca
     7141 agttgccttc gatcatgtcg gtgctgccag agctcggcca caacgacatc gtcggtgtgc
     7201 acgcgggcca cgacgcgctt cgcgacgcga tcctgctcgc gttgcgcgtc gaaggcggcc
     7261 atcccgcgca ggaggcccgc ttgacggcgg ccctcgacat cgccggtcgc agcgtcggcg
     7321 cggtcgaggt cgtcaccatc gagtcgtcct cgccggccga gcggctgatc gagtcgctga
     7381 tcctcgccga cttcgtcagc gtgtacaacg ccgtgcttcg cggcgtcgac cccagcccca
     7441 tcgaagccat cgacctgctc aaggccgcgc aggcctgacc tgcccggatc tgcgcatgcg
     7501 gcggcgggcc actaggattc atctcgaatt ccgatgaaag gacgacgatg aacggccata
     7561 ttgccgaccc aggtctcgcc gagaagggac ggatccgcat cgactgggcc gaccagcaga
     7621 tgccggttct gcgcgagatt cgcgcccgct tcgccaagga gaagccgctc gccggcctca
     7681 agatcgcggc gtgccttcac gtcacgaccg aaaccgcgaa cctgatgcgc gcgctcgtgg
     7741 ccggcggagc cgacgccgtt ctgtgcgcct cgaacccgct gtccacccag gacgacgtcg
     7801 ccgccgcgct cgacgcggac ggcatcccga cctacgcgat caagggcgag gacaacgaaa
     7861 cctactattc gcacatcgag tcggtgatcg ccttcgggcc gcagatcacg atggacgacg
     7921 gcgccgacgt cgtctcgatc cttcactcca agcatccgga caaggccgcc gcggtgctcg
     7981 cgggcaccga ggagaccacg acaggcgtga tccgcctgcg tcagatggcc gccgagaacg
     8041 tgctgatgta tccgatcgtc gccgtcaacg acacgcccac caagcacatg ttcgacaacc
     8101 gttacgggac cggccagtcc acgctcgacg gcatcacgcg cgcgacgaac atcctgtggg
     8161 cgagccgcac ggtcgtggtg tgcggctacg gcttcggcgg caagggcctg tcgatgcgcg
     8221 cgcgcgggct cggcgcgcag gtcatcgtca ccgaggtgga tccggtcaag gcgctcgagg
     8281 ccgtgatgga cggctaccgg gtgatgccga tcgccgacgc cgcgcgcgag ggtgacgtgt
     8341 tcatcaccgt caccggcgac cgggacgtgc tgcgcggcga gcacttcgag atgatgaagg
     8401 acggcgcaat cctggccaac accgggcatt tcgacgtcga gatcgacaag aacgcgctgg
     8461 cgtcgctcgc caccgacgag ccgaagaaga tccgcgactt cgtcgaggag tttcgcatga
     8521 gcgacggccg gcgcctctac ctgctcgcgc agggacggct ggtcaacctg gccgccgccg
     8581 aagggcatcc ggctacggtg atggacatgt ccttcgccga tcaggcgctg accgccgagt
     8641 ggctcgcgaa gggccaggtt ccgctcgaga agcgcgtcta cgacgtgccg cgcgagatcg
     8701 acgaggaggt cgcgcggctg aagctgcaga cgctcggcct gcgtatcgac gagctcaccc
     8761 cggagcaggt gaagtacctg tcgtcgtggc gcgaaggcac gtgaccgccc cacgctgatc
     8821 gcgacattgg gtcccgcgag ccccatatcg ggcacgcggg acctcttttt tcctcctcat
     8881 cccttaaagc aggtgggacc ggctccgagg aacagggcga gcaccgaatg cccggccggg
     8941 gccggccggg ttcgtcgtcc gggctcagga ggaacgccga agccgtcatc gctccgatct
     9001 cccgagcctg aaggaggagt cgaagcatgt cccggaaaac cactcgacgc acggcgcggc
     9061 ggctcttggg tgtcgccgtc cttgccgggc tgtccattct cacgagcacc accgcgcgag
     9121 ccgccaacgc gtccgcgacg tcgctcgcgg cgtcgaaggt caagggcgac agcgcgcagt
     9181 tcccggtgtt cggcgtcacg ctcacgcagc ccgccgaggt ggactcgctc accgacgtcg
     9241 tcgtgcgttt ccagagcatg gtgaacttct caacgggaga tctggccccg gatttctcgg
     9301 ctttcggctg caccaacggc gatgcgacct cctgcgacgg cgtaccgtgc gcatcgtcgc
     9361 gctgggaacg cgcagtgcgg cctcacgtgg cacgagcgtc gccaccgacc ggtacgcggt
     9421 gggctagcga ggcgagcagc cggaagcagg cggcggcgcc gaggcagggc ctgggcgccg
     9481 ccgctcgcgt tcccgccccg ggccgggacg gaccggtgcg ggcctagtca tttcgggcgg
     9541 tattccggaa gaagtgaaac cgtggcgggc cggcctcgtt ctcgcttgcg atgcgccggg
     9601 cctcccttcg cgcgttcgcg ctcgctctcg ccgcggtcac ggccgcggcc ttggcggctt
     9661 cgcctgccgc gaccgcggcc gccttcgggt ccagggccgg cggcaagctg atcgctcccg
     9721 ggcgcaccgc gcctgcggcc gtcaccgacg gggccggcgg cgtgatcgtg tcctcgcgcg
     9781 acgcgaccga cggcgtcgcg gtggtcaacc gctacgacgc gctcggcagc cctgtgtggg
     9841 accaaggaat cgagtttccc gacgcgtccg gaatccagct cgccgccgac ggcagcgggg
     9901 gagcgatcgt cgcggtccac catccggact ccgcggtgat gaccgtgggc cgcatccttc
     9961 actccggcgc caccacctgg caggcgcgct tcccgtcggg tgcgctggtc gcgggcggcg
    10021 acggcggcgc gtggatcgtg tcgcagtcgg gagagatctt cgccgacggc gtcgacgcgg
    10081 ccggcgcgct tcgcccgcgc cttcaggtca ccacctcgcc ggatgccgag tcccttcccg
    10141 tcgcgatcgg cgacggcgcc ggcggcgcgt acctcgcgtg gcgcgcggcg ctgagcgaga
    10201 cgcagttcgc cctgcggctg cagcacctga cggccgccgg agcgctctgg gcggcgccgg
    10261 ccgaggtcgc cggctcatcc gactcttcga tcacgccgcg gctcgcgccg gacgggcgcg
    10321 gcggcgtcgt ggtgtcgtgg gacgcggccg gcctggtgtg ggtgcacgac tattcctccg
    10381 acggcatccc ggtctgggat caggtcgtga cgatcccggc gaccgagggg accgtcagcg
    10441 cggtcggcgc atccgggggc gaggttttcg tcgcctggac gcggcgtctt cggacgcttc
    10501 cgtcgccgac cgacgtgctc aacgtgtcgt tcgtggcctc gacgggcgcg atgcactgga
    10561 cccgtcacgt cgccgtcgtc gacaacggcg tgcgcggcct gacgatcgac gcggccgtcg
    10621 gcgccgccgt cgtcgcctgg gagcatgccg ccatcccgtt cgcgggcgcc gcgctcgacc
    10681 ccgacctgtc gatctaccgc gttacctccg gcggggagga gacctatccg gcgcaccgtc
    10741 gtccgctcgc ggactcgttc ggccctgagt cgctcggcgc gctcgtcgcc gcgggcggag
    10801 gatccgtcgt cgcggtgttc acgcagacgc cctgcttccg cggtgatgtc tcgggtgtgg
    10861 cgatgcaacg catcgccgcc ggcggcgagc gctcgttccg cgccgacggc gcctgcccct
    10921 aagccccgcc cggcggtaga gtcggaccgt ccgatccaga ccggaaggca gccgcgtgcc
    10981 cgacgaaccc gcatcgagct atcccggtgt ccccgctccc tctcagaacg gccatcgccg
    11041 gccgtcccgc gcgccggtgc gcgacgagcg gccgctgcgc ggcgtcgtgg cgggcacgct
    11101 cgccggcgcg atcgcggccg cgatcgcgat cccccgggtg ctgctgctga acctggcctt
    11161 gttcaacccg tggccgcggc ccgtcatcgc ggccgcgctc gcggggctgg tgacgggtgt
    11221 gatgcggcgc ggcgggcgcc ggcgcggcgc gatgatcgcg ggtgcggccg ccgcatcgct
    11281 gggcttgctc gtggcttatg cgggtgttcg gttctcggtc cgggttctgt gggtcgaccg
    11341 caacccggcg gcggtgatcg cggcggacgt gttccggctg accgcctacg cgatcgtgcc
    11401 gggcgcgctc gcgggagcgg cgggatggtt gcttcggggg gccggccgcc gtgcacgaaa
    11461 ccgtcgcgcg atcgcgaaac ccccgatcgt gcgcctctga acaccggtac actcgctgcc
    11521 gtgaccgagc ccgctgcatc gccgatggcc gatgcgcttc gctacgacga gggcgcgatc
    11581 tatgcgatcg accagaccgc ccttcccgcc aagaccgtcg tgcgcaagat caccacgacc
    11641 ggccagctcg ccgaggcgat cctcaagctg cggatccgcg gcgcgccgat gctcggcatc
    11701 gcgggcgctt ggggcgtggc gcaggccgct cgccgggcgg gctccaagcg cggcgcgacg
    11761 cgcaacgccg tgatggcggc ggcgcaacgc gccggcgagc gcctcgccgc gacccgcccg
    11821 acggcggtca acctcggatg ggcgatcgag cgaagcctgg gacgcgcgcg gctcgaagcg
    11881 gcggccggcg cgccgcccgc gacgattgtg aaggcgctcg aagaggaagc ggccgcgatc
    11941 gcatccgagg acgccgccgc atgcatggcc atggccgaga cgggccagcg gttcgttcgc
    12001 ggcggagcgc ggatcatgac gcactgcaac accggttcgc tgtgtaccgg cggctacggg
    12061 accgcgctcg gcgtgatccg gctcgcgcac gagtccggta aggacatcca cgtgctcgcc
    12121 ggcgagaccc ggccgctgct gcaaggcgcg cggctgaccg cgtgggagct cggccgcctg
    12181 cagatcccgc acgcgatcgt gcccgacggc gcgggcccgg cgctgatcgc gcgcggcgaa
    12241 gtcgatctcg ttctcgtcgg tgcggaccgc atcgccgcca acggcgacgt ggcgaacaag
    12301 atcggcacgt actcgctcgc ccttgccgca accgagtcgc aggtcccgtt catcgtcgtg
    12361 gccccgatgt ccacgatcga tccgtccacg cccgacggcg cggccgtcga ggtcgaggag
    12421 cgcgacgggc gcgaggtggt ttccgtgctc ggccggcgcg tcgcgccgcc cgggtcgggc
    12481 gcccgcaacc cggctttcga cgtcaccccc tcgcggttga tcagcgcgat cgtcaccgaa
    12541 cgcggggttg cgatgcggcc gtatcgaacc agcctcaagc ggctgctcgc ccaatagccg
    12601 cgcacccctc gatccggccg ccgcgtcctt gaacgcatcc ggcggggcgc gcatgctcgc
    12661 gtcatgccgc tgctggatct tttgtatccg cgcgtgtgcg ccgcgtgcga cgcgcacggc
    12721 cgggcgccgc tgtgcgcggc gtgcgacgcc ggcgcaccct ggattgggga tgcgtgcctt
    12781 cgatgcgcgc tgccgttgtg ccgcgggtgc gccggccggg ggcttgcctt cgaccgggct
    12841 gcgtcggccg cgatctatgc gggtgcggcg cgcgatgcgt tgatgcgatt caaggccgcg
    12901 ggcgagcatc gcgtcgcggc cgcgctcgcg gcccggatga ccggcgccgc gcggcgcgcg
    12961 ggcatcggcg ccgccggcga caccgtctgc acatgggttc cttcgggtcg cgccgcgttg
    13021 cgcgcgcgcg gacgcaaccc cgccgagctg ctcgcgcgtc cgctggcccg ctcgctcgcg
    13081 gtggcggccc ggcctctgct ggtcaaaacg cgcgagacac cggatctggc cggcctcgcc
    13141 cgcgccgccc ggcggcgcgc cctcgaggga gtcttcgcga tcgccgctcc cgtgccgccg
    13201 cgggtgttgc tggttgacga cgtcttgacc accggaacca ccgcgtcggc ttgcgccggg
    13261 gcgctcaagg ccggcggcgc gtcgcgtgtc gatgtggtga cgtttgctcg cgcgctgtag
    13321 acgcccccgg ccgtcgctat cgtagaatcg cagtccttga ggttccgttc gggtccgtgg
    13381 ttgcggccgg cgaaagccgg cttagtcgat gccagtggca ggcgaaacga cccacgtaag
    13441 gtggccggtg gccgccgagc atggtgccgc ttaggagtaa gccctgccgc ggcgggctcg
    13501 ccgagtccgt cgcgagagga gttccagtgg gtgcggcccg gatccgtcga ggatccgggg
    13561 gtgcggcgtc atccgcatcg ggccgcgcgt agcgacgctc ccggcaggcg agtgtggggg
    13621 caaagaccag gtcggccgga cggaacctcg agcaccgtcc acgaccgcga agccggaccg
    13681 acgccgacga tggaggccac gcgatgcagg tgatcgtcaa agcgcgacac acgcgagtcc
    13741 ccgcagccct caagaagatg gcgaccgaaa aggtcgagaa ggttcgtcgc ttcttcgacc
    13801 ggatcgacaa gatcgagatc gagttctccg aggagcacaa ccctcgcgta gccaataagc
    13861 acgtggtcga agtgaccctg tccgcaaaga accacgtcct tcgggccgtc ggaaaaggag
    13921 cagacccggc gagcgcggtg gatcaagtcg tggacaagat cgagcgtcag gtgcggcgca
    13981 tgaaggacaa gatggtcaaa cggggccgcg cctcggccaa aaccgagact atccgaaag
//
LOCUS       DATLFO010000244         7337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_74971, whole genome shotgun sequence.
ACCESSION   DATLFO010000244 DATLFO010000000
VERSION     DATLFO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74971"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VM841_09950"
     CDS             complement(<1..112)
                     /locus_tag="VM841_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL90541.1"
                     /translation="MFDRVDHVGIAVSDLEAAIAFYKRAFGVEPSHRETVE"
     gene            complement(146..1015)
                     /locus_tag="VM841_09955"
     CDS             complement(146..1015)
                     /locus_tag="VM841_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90542.1"
                     /translation="MAILASVLPRGATAASSPWAPVSMTSYSAKLVCQQAGQGPVRLA
                     GPWRVPDRRTLLWGAEFPYEFHGGLRGDNCALLLGPAAAVARSTMIDVPAHSGATPFK
                     AFVECWHSVPRRSEENPMGTYNLFRRYPVPAAGVIVARFQIGQGDLCRISAGPDAAWP
                     WYMFPGPMYGSYGGVGSPPSDFVARDVFKCMPDGSASHCLFRIRTTPHTRARFLWDSS
                     FTGRLRMSERSGPWTRVTECDVLNGFEVHCAETVSPQRRPTGGITIVAGEAVARAWGT
                     WTVGADSRPVWPA"
     gene            1163..2338
                     /locus_tag="VM841_09960"
     CDS             1163..2338
                     /locus_tag="VM841_09960"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVL90543.1"
                     /translation="MASVIVSGARTPIGKFLGAFSGFTAMDLGGFAIAEALKRAGMSG
                     EQIDYVVMGQVLQGGQGQITARQAATKGGVPMNTPAITINKVCLSGLNAVHLADTLIR
                     AGEAEIVVAGGMESMTNAPYVMPKARQGARMGDAKLVDSMIHDGLWCAFDNKHMGDSS
                     DDVNKKLGIGRAEQDEWAARSHERAADATKAGRLAEEIVPVAVPQRKGDPILVEEDEG
                     IRPGTTVESLAKLAPAFQKDGTITAGSASQISDGGAAMIVMSEEKAASLGLTPLATIV
                     AHGQVAGPDASLHMQPSNAIKAAASKAGLDPAGLDLYEMNEAFSAVALASGNDLGVSD
                     DKVNVNGGAVALGHPIGMSGARLILTLAFELKRRGGGTGAAALCGGGGQGDALIIKV"
     gene            2339..3439
                     /locus_tag="VM841_09965"
     CDS             2339..3439
                     /locus_tag="VM841_09965"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HVL90544.1"
                     /translation="MDEQIVALTRWWSWRRQRLDRSCRGIDDCLRSVVGIPGVHPSAP
                     LSLLARVPRLMQGMYEGAIEGRVALRLPAMRRQPHILHGETAHLVYHACRLGSPRKNG
                     TADPAMRLIRGDVLRAAREPRTAEELRRGVKGAPERFVPLLRQMTLDGQLLRMRAESV
                     WSNDFRYVATRGWLGHTMPPAEPDDALIWLAGEYITTYGPATVHDFAWWTGLDIELAD
                     EALGAHDLADVGYGMKMWRKDARALDLARPVAGRVNLLPAMDPYTMGYIGESRRRFAD
                     DERVLAAMYDKNGNSGNVVLIEGEVGGLWDLQQTKAGAVEMRIGLFDDPGPKMWAAIQ
                     SEAALMSGFLDARDFKVVRAKVRLPGAKKSRS"
     gene            3581..5488
                     /locus_tag="VM841_09970"
     CDS             3581..5488
                     /locus_tag="VM841_09970"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVL90545.1"
                     /translation="MKHAERQWSARPRLALALQITIVAVPIAASVATGSAISRLIPMP
                     PGAGAVLWWIGLFSASTVVVYATERIARRALPLVALYRLSLVFPGKAPSRSRVARRSA
                     SSKEDIEAEVVRAWREGLDADPQRAAETILTLVTALSTHDRRTRGHSERVRMFADLIA
                     AEMDLGADDRNLLRWAALLHDIGKLRVRTETLNKPDRLDEREWAEIKRHPIDGVRLLA
                     PLDGFFGPWMGAIMDHHEKYDGTGYPNNVAGDEISLGARIVAVADAYDTMTASRPYKK
                     PVSPAEGRRELAACAGTHFDPAVVRAFMNISIRRLRWAIGPVSWITQIPFVSRTMEAV
                     TAKTAGAAVTVGSLGGVAGFGIVAPSDLARPAPKTSLAAEQSAAQPASSAGSPKQPRT
                     PGGQAEPPGRTRKGPDPNPKSDESAKPEGPPGHDEPAELVEPQSSPTPTTTPRPSPSP
                     SPQEKKLLLSSSGPGDVAPSSSLPLVSASPPSQPLYNYDVGRDSLPGLKLERAEAVPT
                     EADPATYQIWHAPGGFQISGSTTMWIWAAMDRFEAAEFASVDATLMHCGTYPSCITIA
                     TKNVAGSFWSNDGSWRKLTFDFGTITAAVPADRSIVVHLRVRNVSDDAMMFAYWTATY
                     PATLKTTVRPL"
     gene            complement(5485..6729)
                     /gene="ccrA"
                     /locus_tag="VM841_09975"
     CDS             complement(5485..6729)
                     /gene="ccrA"
                     /locus_tag="VM841_09975"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HVL90546.1"
                     /translation="MTTDIYEIGDIPPLGEVPRRMYAQLIRPERFGEPEKAFAVEELD
                     IPDVGPGETLVLVMAAGINYNNVWAARGVPIDVLKVHGKFGEPTDFHIGGSDASGIVY
                     KVGEGVTNVKVGDRVVIHCGMWDADDPVATSGGDPMFGNSFRIWGYETNWGSFAQFTK
                     VQAHQCMPKAPHLTWEAAAAPTLVGATAYRMLHGWPPNVVREGDPILIWGGAGGLGSM
                     AIQIAAAAGARPVAVVSSEEKGEFCKRLGAVGYINRKDFDHWGMLPHWTDTEAYAKWM
                     AGAKAFGKKWWEVLGSKDAPKIVFEHPGEDTVPTGIFLCDTGGMVVVCAGTTGYNAVV
                     DLRYLWMRQKRFQGSHFANDEQSYAFNQLVLDGKIDPCLSKTFSFEEIGTSHQLMFEN
                     KHPEGNMAALVSAKTTGMRDLD"
     gene            complement(6881..>7337)
                     /locus_tag="VM841_09980"
     CDS             complement(6881..>7337)
                     /locus_tag="VM841_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HVL90547.1"
                     /translation="MDPSYDYTTFNEVVRSVADAYEQAGVTDPRAEIAMAEVHDCFTP
                     TELVLMEDLGFAERGFGWKEVLAGTFDLDGELPVNPDGGLKAFGHPIGASGLRMLFEC
                     WLQLRGEAPPERTIASITAGRKLALTHNLGGGPGECVSFVGVFGSEPSA"
BASE COUNT         1236 a         2550 c         2433 g         1118 t
ORIGIN      
        1 cttctaccgt ctctcggtgc gacggctcga cgccgaacgc ccgcttgtag aacgcgatcg
       61 cggcctccag gtcggacacg gcgatcccca catgatcaac tcgatcgaac atggcgccaa
      121 ttctatccgg aggcgcggcc ggcgatcagg ccggccagac gggccgggaa tcggccccga
      181 ccgtccacgt tccccacgca cgcgcgacgg cctcgcccgc gacgatcgtg atgccacccg
      241 tgggacgtcg ctgtggcgaa accgtctcag cgcagtgcac ttcgaatcca ttcaacacgt
      301 cgcattccgt gaccctcgtc catgggccgc tccgctcgct cattcgcaac cgtcccgtga
      361 aagacgagtc ccacaaaaac cgcgctcgcg tatgcggtgt cgttcggata cggaacaggc
      421 aatgcgaagc cgatccgtcg ggcatgcact tgaagacgtc tcgggcaacg aagtccgacg
      481 gcggtgaacc gacgcctcca tagcttccgt acatcggtcc ggggaacatg taccacggcc
      541 acgctgcatc gggtccggca ctgatgcggc acagatcgcc ctgaccgatc tggaaccggg
      601 cgacgatcac ccccgcggcc ggcacgggat agcgacggaa caagttgtag gtgcccatgg
      661 gattctcttc ggaccggcgg ggcacactgt gccagcactc gacgaatgct ttgaacggcg
      721 tggcccccga gtgagcaggc acatcgatca tcgtcgaccg cgcgaccgcg gccgccgggc
      781 ccagcaacag cgcgcaattg tccccgcgga gtccaccgtg aaactcgtag ggaaactcgg
      841 cgccccacag cagggtccgt cgatcgggga ctcgccacgg cccggccagg cggaccggtc
      901 cctgaccggc ctgctgacag accagcttgg ccgaatagga ggtcatcgac acaggcgccc
      961 acggcgagga cgcggccgtt gctccgcgag gaagcaccga ggcgagaatc gccacggcga
     1021 cgatcattcg tttcatcacc ctgctcaacg agcgaaccgg aaggccgcgt cgcaccgtcc
     1081 gagatcaatg cccgcaacgc ggcatgattt cgcaaagcgg acgcggatac aatggatgga
     1141 tcgtctttca aggaggattt ccatggccag cgtgatcgtt tccggtgccc gcactccgat
     1201 cggcaagttc ctgggtgcct tctccggctt cacggcgatg gacctcggcg gctttgccat
     1261 cgccgaggcg ctcaaacgcg cgggcatgag cggcgagcag atcgactacg tcgtcatggg
     1321 ccaggtcctg caaggcggcc agggtcagat caccgcccgc caagcggcca ccaagggcgg
     1381 cgtgccgatg aacaccccgg cgatcacgat caacaaggtc tgcctgtcgg ggctgaacgc
     1441 ggtgcacctg gccgacacgc tgattcgcgc cggcgaagcc gagatcgtcg tggccggcgg
     1501 catggagtcg atgaccaacg cgccctacgt gatgcccaaa gcccgccagg gcgcccgcat
     1561 gggcgacgcg aagctggtgg actcgatgat tcacgacggc ctgtggtgcg ctttcgacaa
     1621 caagcacatg ggcgactcgt ccgacgacgt caacaagaag ctgggcatcg gccgcgccga
     1681 gcaggacgag tgggccgcgc gcagccacga gcgcgccgcc gacgcgacca aggcgggtcg
     1741 cctggccgaa gagatcgtcc cggtcgcggt gccgcaacgc aagggcgacc cgatcctggt
     1801 cgaagaagac gaaggcatcc gtcccggcac caccgtcgag tcgctcgcga agcttgcgcc
     1861 cgcgttccag aaagacggca ccatcaccgc cggaagtgcc tcgcagatca gcgacggcgg
     1921 cgccgcgatg atcgtgatga gcgaagagaa agccgcatcg ctgggcctga cgccgctggc
     1981 gacgatcgtc gcgcacggac aggtcgcggg cccggacgcg tcgctgcaca tgcagccgtc
     2041 gaacgcgatc aaggccgcgg cctccaaagc cggcctggac cccgcgggcc tggacttgta
     2101 cgagatgaac gaagcgttct cggccgtcgc gctcgcctcg ggcaacgatt tgggcgtgag
     2161 cgacgacaag gtcaacgtca acggcggcgc cgtcgcactc ggacatccga tcggcatgag
     2221 cggcgcgcgc ctgatcctga cgctggcctt cgagctcaag cgtcgcggcg gcggcaccgg
     2281 cgcggctgcg ctgtgcggcg gcggcggtca gggcgacgcg ctgatcatca aggtctgagt
     2341 ggacgagcag atcgtcgcgc tcacacgctg gtggtcgtgg cggcgccagc ggctggatcg
     2401 gtcctgccgc ggcatcgacg actgcctccg gtcggtcgtc ggcattcccg gcgtgcatcc
     2461 gtcggcgccg ctgtcgcttc tcgcgcgggt gccgcgcctg atgcaaggca tgtacgaggg
     2521 cgcgatcgag gggcgggtgg cgctgcggct gccggcgatg cgccgccagc cgcacatctt
     2581 gcacggcgag accgcgcatc tggtctatca cgcgtgccgg ctcggctcgc cccgcaagaa
     2641 cggcacggcc gatccggcga tgcgcctgat ccggggcgac gtgcttcgcg cggcgcgcga
     2701 acccaggacc gccgaggagc ttcgccgcgg ggtcaagggc gcacccgagc gcttcgtgcc
     2761 gctgctgcgg cagatgaccc tcgacgggca gctgctgcgg atgcgggccg agtccgtctg
     2821 gagcaacgac ttccgctacg tcgcgacgcg cggctggctc ggtcatacga tgccgcccgc
     2881 cgagccggat gacgcgctca tctggctcgc cggcgagtac atcacgacct acggccccgc
     2941 gaccgtccac gatttcgcct ggtggaccgg cctcgacatc gaactcgccg acgaggcgct
     3001 cggtgcgcac gatctggccg acgtcggcta cgggatgaag atgtggcgca aggacgcgcg
     3061 tgcgctggat ctggcacggc cggtcgccgg acgcgtgaac ctgctgccgg cgatggatcc
     3121 gtacaccatg ggctacatcg gcgagtcgcg ccggcgattc gccgacgacg agcgcgtgct
     3181 cgccgcgatg tacgacaaga acggaaactc cggcaacgtc gtgctgatcg aaggcgaagt
     3241 cggcggcttg tgggatcttc agcagacgaa agcgggcgcg gtcgagatgc gcatcggcct
     3301 gttcgacgat cccggcccga agatgtgggc cgcgatccag tcggaggccg cgctgatgtc
     3361 gggcttcctc gacgcgcgcg acttcaaggt ggtgcgcgcg aaggttcgcc tgccgggcgc
     3421 gaagaagtcc cgctcgtgaa ggagccgcgt cgccgccggc gaaggtccca tgcgggacga
     3481 aggtcccggt ccgggtcgag tagcccggga tccgattcac cgaaacgttt agcgtcggat
     3541 gcctatatgg ctcacccggc gcggctccga tcctggagac atgaagcatg ccgagaggca
     3601 gtggtcggcc cgtccgaggc tcgcgctggc gctgcagatc acgatcgtcg ccgtcccgat
     3661 cgcggcgtcg gtggcgaccg gctccgcgat cagccgtcta atcccgatgc cgccgggcgc
     3721 cggcgccgtg ctgtggtgga tcgggctctt ctccgcctcg accgtcgtcg tctatgcgac
     3781 cgagcgcatc gcccgtcgcg cactgccgct ggtcgccctc taccggctct cgctggtctt
     3841 tcccggcaag gcaccgtctc gctcgcgggt cgcacgacga tcggcgtcgt cgaaggagga
     3901 catcgaagcc gaggtcgttc gcgcgtggag ggaagggctg gacgccgatc cgcagcgtgc
     3961 ggccgagacg atcctgacgc tcgtcaccgc cctgtccacc cacgaccgga gaacccgcgg
     4021 ccactccgaa cgcgtgcgca tgttcgccga cctgatcgcg gccgagatgg atctgggagc
     4081 cgacgatcgc aacctgctgc ggtgggcggc gttactccac gacattggaa agctgcgcgt
     4141 acgcaccgag acgctcaaca agccggaccg gctcgacgag cgggaatggg cggagatcaa
     4201 gcgccatccg atcgacgggg tccgactgct cgctccgctg gacggattct tcggcccgtg
     4261 gatgggtgcc atcatggacc atcacgagaa gtacgacggc acgggctatc cgaacaacgt
     4321 cgccggcgat gagatctcgc tcggcgcgcg gatcgtcgcc gtcgcggacg cgtacgacac
     4381 gatgaccgcg tcccgtccct acaagaagcc cgtgagcccg gccgaaggtc gccgcgagct
     4441 ggcggcatgc gcgggaaccc acttcgaccc cgccgtcgtt cgcgcgttca tgaacatctc
     4501 gatccggcgg ctccgctggg cgatcgggcc ggtgtcgtgg atcacgcaaa tcccgttcgt
     4561 ctcgcgcacc atggaggcgg tcacggcaaa gacggccggc gccgcggtca cggtcggatc
     4621 gctcggcggc gtcgcaggct ttggcatcgt cgcgccctcc gacctggcga gacccgcccc
     4681 gaaaacctcg ctcgcggccg agcaatcggc cgcgcaaccg gcgtcgtccg ccggatcgcc
     4741 gaagcagccc cgcacgccgg ggggtcaagc cgagccgccg ggacggaccc gcaagggccc
     4801 ggacccgaac ccgaagagcg atgagagcgc caagcccgaa ggcccgcccg gtcacgatga
     4861 gccggcagaa ttggtcgagc cacaaagctc tcccacgccc acgacgactc ccaggccgag
     4921 cccctccccg tccccccagg agaagaaact gttgctgagc tcgtccggac caggcgacgt
     4981 ggcaccgtca tcgagcctcc cgctggtgtc cgcgtcgcca ccctcacagc cgctctacaa
     5041 ctacgacgtc ggccgggatt cgcttcccgg gttgaagctg gagcgcgccg aagcggtccc
     5101 gaccgaggcc gatcccgcca cctatcagat ttggcacgcc cccggcggat tccaaatcag
     5161 cgggtccacg accatgtgga tctgggccgc gatggaccgg ttcgaagccg cggagttcgc
     5221 gagcgtggac gcgacgctga tgcattgcgg cacctatccg tcgtgcatca ccatcgcgac
     5281 caagaacgtc gccggctcgt tctggagcaa cgacggctca tggaggaaac tgaccttcga
     5341 cttcggcacg atcactgccg cggtgcccgc cgaccgttcg atcgtcgtcc accttcgcgt
     5401 gcgcaacgtc tccgacgacg cgatgatgtt cgcctactgg acggccacct atccggccac
     5461 cctgaagacg acggtcaggc cgctttagtc gaggtcccgc atcccggtgg tcttcgccga
     5521 caccagcgcg gccatgttcc cttcgggatg cttgttctcg aacatcagct gatggctcgt
     5581 gccgatctcc tcgaacgaga acgtcttcga caggcacggg tcgatcttgc cgtcgagcac
     5641 gagctggttg aaggcgtagg actgctcgtc gttggcgaag tgcgagcctt ggaagcgctt
     5701 ctgccgcatc cacaggtagc gaaggtccac gaccgcgttg tagccggtcg ttcccgcgca
     5761 caccacgacc atgccgccgg tgtcgcacag gaagatgccg gtcgggacgg tgtcctcgcc
     5821 gggatgctcg aagacgatct tgggcgcatc cttcgagccg agcacctccc accacttctt
     5881 gccgaacgct ttcgcacccg ccatccactt cgcgtaggcc tcggtgtcgg tccagtgcgg
     5941 gagcatgccc cagtgatcga agtccttgcg gttgatgtag ccgaccgcgc cgagccgctt
     6001 gcagaactcg cccttctcct cgctggacac gaccgcaacc ggccgcgcgc ccgctgcggc
     6061 cgcgatctgg atcgccatcg agccgagccc gccggctccg ccccagatca ggatcgggtc
     6121 gccttcgcga accacgttcg gcggccagcc gtggagcatg cggtaggcgg tcgcgcccac
     6181 cagcgtcggt gccgccgccg cttcccacgt caggtgcggg gccttgggca tgcactgatg
     6241 cgcctgcacc ttggtgaact gcgcgaacga gccccagttg gtctcgtagc cccagatgcg
     6301 gaacgagttg ccgaacatcg gatcaccacc ggacgtggcg accggatcgt cggcgtccca
     6361 catgccgcag tggatgacga cgcgatcgcc gaccttgacg ttggtgacgc cctcgccgac
     6421 cttgtagacg atgccgctgg cgtccgagcc gccgatgtgg aagtcggtgg gctcgccgaa
     6481 cttgccgtgg accttcagca cgtcgatcgg cacgccgcgc gcggcccaga cgttgttgta
     6541 gttgatgccg gccgccatca cgagaaccag ggtctcaccg ggcccgacgt cggggatgtc
     6601 gagctcctcg accgcgaacg ccttctcggg ctcgccgaac cgctcggggc gaatcagctg
     6661 cgcgtacatg cggcgcggca cttcacccag cggcgggata tcgccgatct catagatgtc
     6721 ggtcgtcatc ggaagcgtcc tttcggctcg agccacccgg cccggccgag tatagaaagc
     6781 cgcactcggc ccgctcaaac cggacccgga ggaggtccct tcgaagttgc tgtgtgaatt
     6841 cggtgcccga tcctgtcacc ggattcacac agcaccggcg ctacgcgctc ggttcgctgc
     6901 cgaagacgcc gacgaagctg acgcattcgc cgggaccgcc gcccaggttg tgcgtcaagg
     6961 ccagcttgcg gccggccgtg atcgaggcga tggtgcgctc gggcggcgcc tcgccgcgaa
     7021 gctgcagcca gcactcgaac agcatccgca gcccgctcgc gccgatcgga tgcccgaacg
     7081 ccttcagccc gccgtcgggg ttgacgggca gctcaccgtc gaggtcgaag gtccccgcga
     7141 gcacctcttt ccagccgaac ccgcgctcgg cgaatcccag gtcctccatc agcaccagct
     7201 cggtcggcgt gaagcagtcg tgcacctcgg ccatggcgat ctcggcccgc gggtcggtga
     7261 cgccggcctg ctcgtaggcg tcggcgaccg accggacgac ctcgttgaag gtcgtgtagt
     7321 cgtacgacgg gtccatg
//
LOCUS       DATLFO010000245         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_95171, whole genome shotgun sequence.
ACCESSION   DATLFO010000245 DATLFO010000000
VERSION     DATLFO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95171"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VM841_09985"
     CDS             <1..1221
                     /locus_tag="VM841_09985"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVL90548.1"
                     /translation="HSCIVGPVCPTVIIARTEKGHGVKAVENLPGKHGKPLDDEAAAI
                     EELGGMRDIRVEVPRPEFAAEPHVFPTEPLNLPAYELGTKEATRKAFGDALVALGKAD
                     GRIVAIDGEVGNSTYTEDFGKALPDRFVQSYIAEQQMIAYAVGLQVRGWKPYAATFAA
                     FLARAYDFVRMAAISRADINVAGSHAGVSIGEDGASQMALEDLASYRAVHGSTVLYPS
                     DANQAAALVPQMAEREGIVFMRTTREKTPILYEPGEAFEIGGSRVVRSSDDDALTLIG
                     AGITLHEAIKAADLLADEGINARVVDLYSVKPIDVEAIRSAGRETGAIITVEDHWPEG
                     GIGDAVLSALAEEQPHPVVVKLAVAGMPGSGKPAEMLHAAGIDADCIAAAARELVTRT
                     GAQDTNQVGSGRRE"
     gene            1240..>2030
                     /gene="tal"
                     /locus_tag="VM841_09990"
     CDS             1240..>2030
                     /gene="tal"
                     /locus_tag="VM841_09990"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HVL90549.1"
                     /translation="MTGAERDRDEDKAVDQHREAEQVTRKADESSALHRLHAEQGQSP
                     WIDFIDRELIESGKLDEMIEDGIRGLTSNPTIFAKAVATGQYDELIRREIEAGDDAHE
                     IYEQIATSDVGDAADVLRRVYDDANGTDGFASIEVEPNLAEDTEATLARARELWAKLG
                     RPNVMIKIPATPAGIPAVQAAIADGINVNVTLMFSVDVYREVARAYIAGLRQRHERGE
                     DLSRVASVASFFVSRVDGKVDKALNEAGGRAALEARGRAAIANARL"
BASE COUNT          363 a          659 c          729 g          279 t
ORIGIN      
        1 cactcgtgca tcgtcgggcc ggtctgtccg acggtgatca tcgcccgcac ggagaagggc
       61 cacggcgtga aggccgtcga gaacctgccc ggaaagcacg gcaagccgct tgatgacgaa
      121 gccgccgcga tcgaggagct cggcgggatg cgcgacatcc gggtcgaggt ccccaggcca
      181 gagttcgcgg ctgagccgca cgtcttcccg accgagccgc tcaaccttcc ggcctacgag
      241 ctcgggacga aggaggcgac gcgcaaggcc tttggcgacg cgctcgtggc gctcggaaag
      301 gccgatgggc gcatcgtcgc catcgacggg gaggtcggca acagcaccta caccgaggac
      361 ttcggcaagg cgctccccga tcgcttcgtg cagtcgtaca tcgctgagca gcagatgatc
      421 gcctacgccg tgggcctgca ggtgcgggga tggaagccgt atgcggcgac cttcgcggcg
      481 ttcctcgcac gcgcctacga cttcgtgcgg atggcggcca tcagccgagc cgacatcaac
      541 gtggcggggt cgcatgccgg cgtcagcatc ggcgaggatg gggcctcgca gatggcgctc
      601 gaggaccttg ccagctatcg cgcggtgcac ggctcgacgg tgctgtatcc cagcgacgcc
      661 aaccaggccg cggccctggt gccgcagatg gcggagcgcg aaggcatcgt gttcatgcgc
      721 acgacgcgag agaagacgcc gatcctctac gagcccggcg aggccttcga gatcggcgga
      781 tcgcgggtcg tccgctcgtc cgatgacgac gcgctgaccc tgatcggggc cgggatcacg
      841 ctccacgaag ccatcaaggc ggcggatctg ctggccgatg agggcatcaa tgcgcgggtc
      901 gtcgacctgt acagcgtcaa gccgatcgac gtcgaggcga tccgcagcgc cggacgcgag
      961 accggcgcca tcatcacggt cgaggaccac tggccggagg gtggcatcgg ggatgcggtg
     1021 ctctccgccc tggccgagga gcagccccac ccggtggtgg tgaagctggc cgttgccggc
     1081 atgcctggat ccggcaagcc cgcagagatg ctccacgccg cggggatcga tgcggactgc
     1141 atcgcggcgg ctgcgcgcga actggtgacg cgcaccggcg ctcaggacac caaccaagtc
     1201 ggctccggtc ggcgcgagta gcttcgggag aaccttgaga tgacaggagc agagcgggac
     1261 cgggacgagg acaaggctgt ggatcagcac cgcgaggcgg aacaggttac tcgcaaggcg
     1321 gatgagagca gcgcgctgca tcggcttcac gcggagcagg gccagagccc gtggatcgac
     1381 ttcatcgatc gtgagctcat cgagagcggc aagctcgacg agatgatcga ggacggtatt
     1441 cgcggcctga ccagcaaccc gaccatcttc gccaaggcgg tcgcgaccgg gcagtacgac
     1501 gagctgatcc gccgcgagat cgaggccggc gacgatgccc acgagatcta cgagcagatc
     1561 gccacgtccg atgtgggtga cgccgcggat gtgcttcgtc gggtgtacga cgacgcgaac
     1621 ggaaccgatg gcttcgccag catcgaggtc gagcccaacc tggccgagga cacggaggcc
     1681 accctggccc gagcccgtga gctgtgggcg aagctcggcc gtcccaacgt catgatcaag
     1741 attcccgcga cgccggccgg catcccggcc gtccaggcgg cgatcgccga cggcatcaac
     1801 gtcaacgtca ccctgatgtt cagcgtcgac gtctatcgag aggtcgcgcg ggcgtacatc
     1861 gccgggctcc gtcagcgtca cgagcgcggc gaggacctct cgcgcgtcgc cagcgtggcg
     1921 agcttctttg tcagccgcgt ggatggcaag gtggacaagg cgctgaacga ggccggcggg
     1981 cgcgcggcgc tcgaggcacg cggccgcgcg gcgatcgcga acgccaggct
//
LOCUS       DATLFO010000246         6573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_143488, whole genome shotgun sequence.
ACCESSION   DATLFO010000246 DATLFO010000000
VERSION     DATLFO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143488"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VM841_09995"
     CDS             <1..684
                     /locus_tag="VM841_09995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90550.1"
                     /translation="LEGFTRLSLAGLPADSGWPEAIASAAKAANLFPAEEPEDVCPIV
                     AIGGSYDEYLASLTGKQRHEIRRKARRLADAAAVTFRSSTAESLDADLDAFFALHRSA
                     AGPKGKFMHQDMEAYFRRLAHTMQRAGRLRLTILEMDAAPSAMLYGFSDGTRWELYNS
                     AYDFGKRELAPGMVLVAETIRLAAEEGCETFDFLRGAEDYKYRFGAVDRRVVRLLADS
                     AERGSSSRE"
     gene            722..2020
                     /gene="mshA"
                     /locus_tag="VM841_10000"
     CDS             722..2020
                     /gene="mshA"
                     /locus_tag="VM841_10000"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HVL90551.1"
                     /translation="MRHTGEAAPRIERVAVISMHTCPLDQPGTGDSGGMNVAVRSMAS
                     RLAELGVQVDLFTRASGPEQHIDSVDPGVRVVHLEAGPVEPIEKERLPDYLWPFLCGL
                     LRFEAEEAARLGIDGPLYDLVHSHYWLSGWAGRLARDRLNVPLIHSFHTLGAVKNRAL
                     APGDAPEPPLRIRGEEKIVQTADCLIAPTEIEAADLISLYGACPERIRVVSPGVDTDR
                     FRPGVPDAAKASLGLAGRKVVLFVGRLQPLKRPDLAVRAVAELLRRREDLARDVTLVV
                     VGGPSGRGGVSAQSLRELAVSLGVGANVEVRDPVAHVMLPDYYRAADVVVMPSMTESF
                     GLVALEAQACGTPVVATDTGGLRAAVRNGTTGLLVPGSEPAAYARALERLLDDRPLRS
                     TMGAAAARRARGLDWRRAAAAMLDVYEDVLMPAEIASPAP"
     gene            2108..2629
                     /locus_tag="VM841_10005"
     CDS             2108..2629
                     /locus_tag="VM841_10005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVL90552.1"
                     /translation="MSDLPVMIRRVEPADYPLIQRWQNDPEIFSLMDYERPFSAADIV
                     ASEENAARKGIPFIIEAGGTPIGRIGLNGFRHRDRTASLYVFIGEKAAWGSGNGRAAI
                     ELALRYAFGTLNLRLVQLWTLAHNERAIHLYERIGFSEDARLRDRSWIEGRYVDHLVM
                     SITADEFASRSGT"
     gene            2626..3072
                     /locus_tag="VM841_10010"
     CDS             2626..3072
                     /locus_tag="VM841_10010"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HVL90553.1"
                     /translation="MTGDAGAVVEHFLTDRGIEFVRAGNQFSFVLSGENKHRIPVGLA
                     LRADGVRLESFFMRAPRENQSEAYRLLLSRNARSRAVWFALDADGDVFLLGQSAAVNE
                     AELDRLVGELLTTADAIFMPALSLGFAEYLAADLAWREKHAGTAGS"
     gene            3715..5457
                     /locus_tag="VM841_10015"
     CDS             3715..5457
                     /locus_tag="VM841_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90554.1"
                     /translation="MMRSAAVSVVATAMLVLAGIGPVGAAAGPSGIATAENTLASLSY
                     GTATAKAGTDRVTSAWGANPLAEAAMDFLVAGAVKLSGGSVTATGPTESGSETLGTGA
                     KTIEGLAGATVTGASVEAAVGAASASSSAVITMADLSLLNGLVSGTSARTATSSIVTA
                     QSSTVERTLELGDVRVLSLRALLDRLGVDPTSLTCAGVAAAGAELGVDTAAACPSQDD
                     VVAAVDALGAALSAARAQLVADLQTANATIAGLTIVTQPLIDLAARYGQDWSGLAGLL
                     LSGAARDALIAQIDTRLTAVDGGISTAGTGTCAAAQQAASDVSASFPSMAAVLAPLSL
                     AVADACAVLRSLLDDLMGVSLLGLDALTVGLNALARPDTPLAGGSGTIGAITVGNRSI
                     AVAGLTSLSAALQLAIETAKAGTAGALTALGIGAFPEPEIEILGVESTAGRDQNDIWF
                     AHVTVTGLHVALPPVPISLPSGTTLRAVLDAPVTPASAASRVRAAAPVATSPPIVVDV
                     AVFSGEARYRASGPIDPVDPAPVPLNPGPGSGNPAPATGGPLPKTGSGLHWLAVGMAI
                     MAAGAVVRFTLGEG"
     gene            5518..6423
                     /locus_tag="VM841_10020"
     CDS             5518..6423
                     /locus_tag="VM841_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HVL90555.1"
                     /translation="MLRNLILGVANQPLVRRVATGGPGRRVALRFVAGENLDQGFGVT
                     RALCRRGFSVALDHLGENVMSADAADAAAAVYREAIERIAAEGLDATVTLKPTQMGLD
                     IDEGLAHANVAKLCAAAASSGSSLALDMEDHRYTERTIAMCLRLDAEFPGRAGIALQS
                     ALYRTPADLDRVIGVPVRIVKGAYREPPEFAYPRKADVDRAFAQIVARLMREGAYPMI
                     ATHDERLIRYTLRRARQYGRSPDTFEFQMLYGIGRNLQQRLLDQGYRVRVYVPFGSDW
                     YPYLIRRLAERPANLAFFLAALARG"
BASE COUNT          960 a         2263 c         2304 g         1046 t
ORIGIN      
        1 ctcgagggct tcacgcggct gtcgctcgcc ggccttccgg cggactcggg ctggcccgag
       61 gcgatcgcat cggcggccaa ggccgcgaac ctctttccgg ccgaggagcc ggaggacgta
      121 tgtcccatcg tcgccatcgg cggctcctac gacgagtatc tcgcgtcgct caccggaaag
      181 cagcggcacg agatccggcg caaggcccgc cgtctcgcgg acgcggcggc ggtcacgttc
      241 cgttcctcga cggccgagtc actcgacgcg gatcttgacg cgttcttcgc gctccaccgt
      301 tcggccgccg ggcccaaggg caagttcatg caccaggaca tggaggcgta cttccggcgc
      361 ttggcgcata ccatgcagcg cgcaggccgc ttgcgcctga cgattctgga gatggacgca
      421 gcgccgtcgg cgatgctcta cggattctcc gacggaacgc gctgggagct gtacaactcg
      481 gcatacgact tcggcaagcg ggagctcgct cccggtatgg ttctcgtcgc cgagacgatt
      541 cgtttggcgg ccgaggaagg ctgcgagacc ttcgacttcc tgcggggtgc cgaggactac
      601 aagtaccgct tcggtgccgt tgaccgccgc gtcgtccggc ttcttgccga ttccgcggag
      661 cgcggatcgt cgtcgaggga atgatcgaac cggcccggac gtttccggcg aggaggtgag
      721 catgcgtcac actggtgaag ccgcgccgcg catcgagcgc gtcgccgtca tcagcatgca
      781 cacgtgtccc ctggatcagc ccggcacggg cgactccggc ggcatgaacg tcgccgttcg
      841 gtcgatggcc tcccgcttgg ccgagctcgg cgtgcaggtt gacctgttca cgcgggcgtc
      901 cggccccgag cagcacatcg acagcgtcga tcccggcgtg cgcgtcgttc acctcgaggc
      961 cggcccggtc gagccgatcg agaaggaacg gctgcccgac tacctgtggc cgttcctgtg
     1021 cggcttgctg cgcttcgagg cggaggaagc ggccaggctc ggtatcgacg ggccgctcta
     1081 cgacctggtt cacagccact actggctcag cggatgggcc gggcggctcg cgcgggaccg
     1141 gctcaacgtg ccgctcatcc actctttcca cacgctcggc gcggtgaaga accgcgcgct
     1201 cgctccgggc gacgctcccg agccgccgct gcggatccgg ggcgaggaga agatcgtgca
     1261 gacggccgac tgcctgatcg ctccgaccga gatcgaagcg gcggatctga tatcgctcta
     1321 cggcgcatgc cccgagcgga ttcgcgtcgt gtcgccgggc gtggacaccg accggttccg
     1381 gccgggcgtg cccgacgcgg ccaaggcatc cctcggcctc gccggccgca aagtggtgct
     1441 cttcgtcggc cgcctgcagc cgctcaagcg gccggatctc gccgttcgag cggtcgccga
     1501 gcttctgaga cggcgcgagg atctcgcccg cgacgtcacg ctggtcgtcg tcggcgggcc
     1561 gagcgggcgt ggaggcgtct ccgcgcagtc gctgcgcgag cttgcggtct ccctcggcgt
     1621 cggggcgaac gtggaggttc gcgatccggt ggcccacgtc atgcttcccg actactaccg
     1681 tgccgccgac gtggtcgtga tgccgtcgat gacggagtcg ttcgggctcg tcgcactgga
     1741 agcgcaggcc tgcggcacgc cggtcgtcgc gaccgacacg ggcgggctgc gtgcagccgt
     1801 tcgcaacgga accacgggtc tgctcgttcc cggctccgag ccggctgcct atgcacgggc
     1861 gctcgaacgg ctgctcgacg atcgcccgct ccgatccacc atgggggcgg cggcagccag
     1921 gcgggcacgc gggctggatt ggcgccgcgc ggcggcggcg atgctcgacg tctacgagga
     1981 cgtgctgatg ccggccgaga tcgccagccc ggctccctga acgccggccc ggctccctga
     2041 acgccggccc gatcacctga gcgatcgctc cacgccgctg ctggccgctc cgacctagaa
     2101 tgtcctcatg agcgatcttc ccgtcatgat ccgccgggtg gagcccgccg actacccgct
     2161 gatccagcgc tggcagaacg atcccgagat cttttcgctg atggactacg agcggccttt
     2221 ttccgccgcc gacatcgtcg cctccgagga gaacgcggcc cgcaagggca tcccgttcat
     2281 catcgaggcc ggcgggaccc cgatcggacg catcgggctg aacgggttcc gtcaccggga
     2341 ccggacggct tcgctgtatg tgttcatcgg agagaaggcc gcatggggat ccggcaacgg
     2401 ccgtgccgcg atcgagctcg cccttcgtta cgcgttcggc acgctcaacc tgaggctcgt
     2461 gcagctgtgg acgctggccc acaacgagcg tgcgattcat ctgtacgagc ggatcgggtt
     2521 cagcgaggac gcgaggctgc gcgatcggtc ttggatcgag ggccgctacg tggaccatct
     2581 ggtgatgagc atcaccgcgg acgagtttgc ttcgcggagc ggaacgtgac cggcgatgcc
     2641 ggggccgtcg tcgagcattt cctgacggac cgcggaatcg aattcgtgcg ggccgggaac
     2701 cagttctcgt ttgtgctgtc gggcgagaac aagcaccgca ttccggtcgg cctcgcgctc
     2761 cgcgcggacg gtgtccggct cgagtcgttc ttcatgagag cgccgcggga gaaccagtcc
     2821 gaggcgtacc ggctcctgct ttcgcggaac gcccgttcac gcgcggtgtg gttcgcgctc
     2881 gacgccgacg gggacgtctt cctgctcgga cagtcggccg ccgtcaacga ggccgagctc
     2941 gaccggctgg tcggcgagct gctgaccacc gccgacgcga tattcatgcc cgccctgtcg
     3001 ctcggcttcg ccgagtacct cgccgccgat ctcgcctggc gggagaagca tgccggcacg
     3061 gctggctcat aggcccgccg accaggcatc ttgccgggct gggcctcgac caattgatct
     3121 gccgagcaaa attttttctg attcgacaga tgccccaaga cccgtgacct gcggctttgc
     3181 agtgccaaca gggcttttcc cgcccttgaa tgctcgctgg cgcgtggtag gttctcgcat
     3241 gtcccgagcg gccggagcgg ccgaatcagg gaaccgaatg gggtaccggc gggcgggcga
     3301 gccgtaagga gcccaaccga gtgggggtac actggtcggc cggtagggaa agcgaacttg
     3361 ctggtcgggc gggtaaacta ccagaagggt aagtgaccca aagcagaacg tctgttggcg
     3421 ctaccacaag gacaccaaga cggtacgcgg agggttgctc gggacactta gggtccgagg
     3481 agtctgaggg ccgcgcgcgg gaaggtccgc aaggatcgga acgcacggca ggccggtagg
     3541 gttcctcggg ctcttctttt tctcgtccat tagcggacac tttcgccgtg tcaggcatct
     3601 ggcctccgcg gcgcccggcc gccccaccga tgactacggg agatcgcctg caagggctat
     3661 cggccggccc ggccccggcc gccgaaacga acagggatgc gggcaaattc ggggatgatg
     3721 cggagtgccg ccgtttcggt ggtcgcgacc gcgatgctgg tgctggccgg aatcggaccg
     3781 gtgggggctg cggccggacc ctcggggatc gcgacggcgg agaacacgct ggcgtcgctt
     3841 tcctacggga ccgccacggc caaggccggg acggaccggg ttacgagtgc gtggggcgca
     3901 aaccccctgg ctgaggccgc gatggatttt ctcgtcgccg gcgccgtcaa gctcagcgga
     3961 gggtccgtga ccgcgacggg gccgaccgaa agcggttccg agacgctggg aaccggggcc
     4021 aagaccatcg agggcctcgc gggcgccacg gtcaccggcg cgtcggttga agccgccgtc
     4081 ggagcggcca gcgcatcgtc gtcggccgtg atcaccatgg ccgacctgag ccttctgaac
     4141 ggcctggtca gcgggacctc ggcccgaacg gcgacgtcgt cgatcgtgac ggcgcaatcg
     4201 tctacggtcg agcgcacgct cgagctcggt gacgtgcgcg tgctgtcgct gcgcgcgctg
     4261 ctcgatcggc tcggcgtgga cccgacgtcc ctgacgtgcg ccggagtcgc ggccgcaggc
     4321 gccgagctcg gcgtggacac cgccgcggcc tgtccgtcgc aggacgatgt cgtggccgcg
     4381 gtcgacgcgc tcggagccgc gctcagcgca gcgcgagccc aactcgttgc cgacctgcag
     4441 accgcgaacg ccaccatcgc gggactcacg atcgtcacgc agcccctcat cgatctcgca
     4501 gcccgatacg gccaggattg gtccggccta gcgggcctgc tgctgagcgg agccgcacgc
     4561 gacgccttga tcgcgcagat cgacacgcgc ctgaccgctg tggacggcgg catcagcacg
     4621 gccggcaccg gcacgtgcgc ggcggcgcag caggcggcat cggacgtgtc ggcctcgttc
     4681 ccgtcgatgg ccgcggtgct cgcgccgctg tcgctcgccg tcgcggacgc atgcgcggtg
     4741 ctccgctcgc tgctcgacga cctgatgggc gtctcgctgc tcggcctgga cgccctgacc
     4801 gtcgggctga atgcgctggc ccggccggac acgccgctcg cgggtggctc cggcacgatc
     4861 ggagccatca ccgtgggcaa cagaagcatc gccgtggccg ggctcacctc gctgagcgcc
     4921 gcgcttcagc ttgcgatcga gaccgcgaaa gcaggaacag ccggtgcgct caccgcgctc
     4981 gggatcgggg ccttccccga gcccgagatc gagatcctcg gcgtcgaatc gacggccggg
     5041 cgggaccaga acgacatctg gttcgcgcac gtgacggtga cggggctgca cgttgcgctc
     5101 ccgcccgtgc cgatctccct gccgtcggga acgacgctgc gtgctgtgct cgacgcgccg
     5161 gtgaccccgg catccgcggc ctcccgcgtg cgcgccgcgg cgccggtcgc gacctcgccg
     5221 ccgatcgtgg tggacgttgc tgtcttcagc ggcgaagccc gctaccgcgc gtccggtccg
     5281 atcgacccgg tcgatcccgc gccggtcccg ctcaaccccg ggccgggcag cggaaatccg
     5341 gcgccggcca cgggcggtcc gctcccaaag accggctcgg ggcttcattg gctcgcggtg
     5401 ggaatggcga tcatggccgc cggagccgtg gtccggttca cgctcggcga gggctgaccc
     5461 cgaacgcgcc cgcgacggcg catacaatga gcgcgtgacg ggcaaggacg cagggacatg
     5521 ctccgcaacc tgatcctcgg cgtcgccaac cagccgctcg ttcgccgtgt ggcgaccggc
     5581 gggccgggaa ggcgggtcgc gctgcgcttc gttgcgggcg agaacctcga ccaaggtttt
     5641 ggggtgaccc gtgcgctgtg ccgccgaggg ttttcggtgg cgctggatca tctcggcgag
     5701 aacgtgatgt cggcggacgc cgcggacgcg gcggccgcgg tctaccgcga agccatcgag
     5761 cgcatcgcgg ccgaggggct cgacgcgacg gtcacgctca agcccacgca gatgggtctg
     5821 gacatcgacg agggcctcgc ccacgcgaac gtcgccaagc tctgcgcggc cgcggcgtcc
     5881 tccgggtcgt cgctcgcgct cgacatggag gatcatcgct acaccgagcg cacgatcgcg
     5941 atgtgcctcc gactcgacgc cgagttccct ggacgcgccg gcatcgccct gcagtcggcg
     6001 ctgtatcgca cgcccgccga cctggaccgc gtgatcggcg tgccggtgcg gatcgtgaaa
     6061 ggcgcctacc gcgagccgcc cgagttcgcc tatccacgca aagccgacgt ggatcgtgcg
     6121 ttcgcccaga tcgtcgcccg gttgatgcgc gagggcgcct acccgatgat cgcgacccat
     6181 gacgagcggc tgatccgcta cacgctgcgc cgcgcccgtc agtacggacg ctcgccggac
     6241 accttcgagt tccagatgct ctacgggatc ggccggaacc ttcagcagcg actgctcgat
     6301 caggggtatc gcgtccgcgt ctacgttccc ttcggctccg actggtatcc gtacctgatt
     6361 cgccgcctcg cggagcggcc cgcgaacctg gccttcttcc tcgctgcgct ggcccgcggc
     6421 tgaaattcgc gccgcgatcg ggatccggtc ctagaatcaa ggaacaggaa aggggacgcg
     6481 tggacggaac gctggcgatt ctcggtggcg gcaagatggc cgaagctctc ttgtccggcc
     6541 tgctgcgctc aggctggcgc acgccgcaag agg
//
LOCUS       DATLFO010000247        11821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87774, whole genome shotgun sequence.
ACCESSION   DATLFO010000247 DATLFO010000000
VERSION     DATLFO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87774"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1054)
                     /locus_tag="VM841_10025"
     CDS             complement(23..1054)
                     /locus_tag="VM841_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVL90556.1"
                     /translation="MRAARMTGVGKPVEIHEVPVPAPGPGEVLVRVAACGVCASDVHV
                     LDGSLPAMPPLPVTMGHEPSGTVEAAGEGVLWPAPGDRVVMHAGKRCGACRPCRAGHG
                     LEECLFVRTMGVDFDGAWADYVVVPASSCVALPKSIPFDQGAILADAVATPYAAVVET
                     GQVRPGDRVAIFGVGGLGTHAVQIARLCGASFVAAVDPRQSARSRATALGADIAVDPD
                     GAVGAIRDATGGEGVDAAFDFVGANAVVKACVSSLARGGRAVIVGVGGEKISLGPSIL
                     FALKHSQLRGAYGYRRTHIETLVSLVASGRLDLSASITANMPLEQAADAVEILATKRG
                     DPVRIILSP"
     gene            complement(1060..2037)
                     /locus_tag="VM841_10030"
     CDS             complement(1060..2037)
                     /locus_tag="VM841_10030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL90557.1"
                     /translation="MSLAAFISGGKSLGDAVSRVQLAESLGYEAVFSTHTTGRDGLMT
                     LAAYAAATKRIRLGTGVVPAFPRHPLAMAIEAATLDEMSDGRLILGVGPSHKVTMEGW
                     YDISMAKPLSQMREYVQILRSVFTTDGVTFKGEFYNTQFGFVGYGARKDLPIYLSALA
                     PKMLSFAGEACDGVVLWGCMPSYIASTVAPTVRAAAEKAGRPVPTIVAAVPTAVTTDK
                     QAAFDALRGDFFVYMTLPFYRRAIAAAGYQAEIDAFDKAQAAKDFPGALAAMSEAMLT
                     EFAAIGDAAAVRDKLDEYRAAGATLPAVGLFRGGDGYAGPEETLKAARG"
     gene            complement(2040..3215)
                     /locus_tag="VM841_10035"
     CDS             complement(2040..3215)
                     /locus_tag="VM841_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90558.1"
                     /translation="MSIIAIPQELLELRTSILDFIDREIRPVEAKYSQELQETGTIAS
                     AEEEKRAIRKRSAELGFYTLHMNEEVGGAGLSYLGQCLMHEAIAASGSFLAGRGGVMA
                     SVEGPTPVLHACTPEQRERWLDPLMRAEREMCFALTEPGAGSDATRIKTKAERKGDSY
                     VINGTKHFITHGEEADFALVFAVTDAEKRSRGGITAFLVDKDTPGFTVARRQHTASTV
                     DKPAELVFNDVEVTSENILGQEGYGFAAAMAWINGGRLSIASQAVGYAQLLTDKMLDY
                     AKSRVAFGSTIGSYQYVQGMIVDSLNELEQARLLVYKKADEIDNGADGRREAAQAKVV
                     ATEMVGRVADRAIQVYGGNGFMTEMGIERYWREVRAMRLYEGTSEILRSNIAKTLGL"
     gene            complement(3356..5662)
                     /locus_tag="VM841_10040"
     CDS             complement(3356..5662)
                     /locus_tag="VM841_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVL90559.1"
                     /translation="MPKSQRLGEVLLEAGLITQDVLDAALVHQRQGEDAAGRRVRLGT
                     VLLDMGVVSEEGLAHALSMQLGIPFVDLTSVAVDPDLLQLIPRRTADRLGVVPVDRTE
                     AGILIAMADPTDVVALDDVRVTARAGTIRAAVATQSAVRDAIDRFYSLDSATTDVVNR
                     LGEAADVEVVVATAPEEPVEDLASSGQAAPIVRLANAILADGVRARATDVHIEPQQSE
                     VTVRYRIDGLLREVMTLPKTVHAALVSRLKIMATMDIAERRRPQDGRARVVVSGSDVS
                     LRVSTIPTMRGEKVVLRLLRQGEETVSLEETGMTLDQLALVRDALSQPQGFVLFTGPT
                     GSGKTTSMYATLLEKKSPEVNIVTLEDPIEYQIRGISQMQIDDKTGLSFARGLRSVLR
                     QDPNVIMVGEIRDVETAHISLQAAMTGHLVFSTVHTNDAASAVTRLVDMGVESFLVAQ
                     ALSLIVAQRLVRVICPHCKQEAPPSEAALKRLGLAPDTLAGVTLYRGAGCETCGFTGF
                     LGRTGIFEVLPIRRQMREQINAQAAEGVLSAGARAAGVRSLLEAGLEKVRAGITTLDE
                     VQREVQIERQPDRPRCPSCRNEVDPAFVVCPYCQLNLGPASCGSCGREVAADWNICPY
                     CRAELDPAAPAAANPHTPGRARVLFVDDDPSLIQLAHAMFDDDFEVLDASSGEDAIRR
                     AAVERPDLILLDLHMPGIGGLEVAKRLRGSAATSMIPLIMLTGDDSGEIEGLRAGVDD
                     YIVKPFDEDRLRARIERALRLAARVATG"
     gene            complement(5764..5848)
                     /locus_tag="VM841_10045"
     tRNA            complement(5764..5848)
                     /locus_tag="VM841_10045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5811..5813),aa:Leu,seq:cag)
     gene            5991..6704
                     /locus_tag="VM841_10050"
     CDS             5991..6704
                     /locus_tag="VM841_10050"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HVL90560.1"
                     /translation="MGILKDFERRLEQAVEGFFARALPGGGLQPVELGKRMVRAMDDA
                     ASVGVAGDVIAPNAFVFRLAPPDHERLSGLGATLERELVAVARRAATAEGWVLLGPPV
                     IELVADPDMTPGTFMIATAVEESDDPAAGAGAHTQLIQIAVASDAELVVLGSKRRAYP
                     LSKDTLVIGRLDECDITLSDAGASRRHAEVRREGDEWYVVDLDSTNGTLLNGKSVRRA
                     RLNPKDVILIGNTQVEYRA"
     gene            6721..7194
                     /locus_tag="VM841_10055"
     CDS             6721..7194
                     /locus_tag="VM841_10055"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVL90561.1"
                     /translation="MPELVLTSLKFLFLALLYLFVARTVRVIWLDIAGPATGGRRQAV
                     AQAPRARPAARGGRGAVPRSIAVVEENKSPKVLPLDQDAITLGRAAECSVALDDTYVS
                     QLHTKIFRKDGAWFVMDLGSTNGTYLNRMKVSDPMPVSSGDEVRLGKTVVEVRAK"
     gene            7191..8402
                     /locus_tag="VM841_10060"
     CDS             7191..8402
                     /locus_tag="VM841_10060"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HVL90562.1"
                     /translation="MSRTAGLIRWAAATDVGRARQLNEDSWFGGERIYAVADGLGGHR
                     AGEVASRMAVEELARLDRKPPDGDIGAELVEAVRAAGRAVAQRSAGDPETAGMATTVT
                     AIAMDGGAAHLAHVGDSRCYLLRGEQMTLVSTDHTLVARMVDEGAITLEQAETHPQRS
                     VLTRALGTDPEVDVDHQVIPLAPGDRILLCSDGLSSVVPEPEIAGLLASVRDGDDLCR
                     ALVDEANRRGGPDNITVLIIDVPADAQLPVYDGTSSMAALPEEPVRRPVAPPPARRTR
                     RVPVRLIVWLSILVLAVAGGLGGLRIWSDRNWFVGFDGDQVAIYRGLPTDFWVSLHRV
                     HEPTALTRDQVEPRYVERLEEGLRASTLEEAREIASRIPRAPTPTPSPTPSPTRSPSV
                     LPSPSPLGSPT"
     gene            8399..9817
                     /locus_tag="VM841_10065"
     CDS             8399..9817
                     /locus_tag="VM841_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HVL90563.1"
                     /translation="MNRRSAEAGLIAMAVAISLGALLLVAFAQSAEVPPDFFGHAVFV
                     AAFAVGMHLIVRKAAPSADPVLLACAIALTGVGYAVIERLDPDLAVSQALWLAIGGAA
                     FAATLLLLRDHRTLEKFRYSLMLLGVALLLLPLAPVIGKTVRGARLWVAVGPMTFQPA
                     EAAKIVLAAFLAGYLASKREVMTVPTARIGPLKLPAPRHFGPLIIAWVLSLAIMVFQR
                     DLGSSVLFLALYIVTIYVATSRASYVVAGVGLLAGGIAFASMAFSHVASRFSVWLDPW
                     SDIDGKGFQIAQSLFALGTGGVAGEGLGRGRPDFIGKSSIVATDSIFAAIGEELGLIG
                     TVGILMLFAVMVARGFHIALRSRDSFGALLATGLTAIIGVQTFLIVGGVTRLIPLTGI
                     TLPFVSYGGSSIVANFVLIAILMRISNIEAGTHAEAAEARGGARAGGAAVATTDVQGR
                     DEAPLADLTSVHRIDVPPENRS"
     gene            9814..11295
                     /locus_tag="VM841_10070"
     CDS             9814..11295
                     /locus_tag="VM841_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVL90564.1"
                     /translation="MNRSIRRVALTLGVAFLALLVQLNYDQVVASKRFAEHPANRRLL
                     VKEYSTQRGDIVASGQIVARSVATDDKLKYLREYPLAALTAEISGYYSLVYGGAGIER
                     AYNSYLTGNKPARVESIVDQLLGRDRKGNSLMLTLDLELQKVAAEKLGKQRGAVAAID
                     PQTGAVLALYSSPTYDPNALSSHNTGAIRRSWEFYQNAEHNPLLSRSFQERYPPGSTF
                     KVLVAAAALENGIKPETAYPNPRAFPLPLTNRELGNFGGGACRGGSGGTITMAQGLRV
                     SCNTTFAQIGLQIGADKLVAQAEKLGFQQRPVFDLPAVPSCIRTAARGCADPDLDAPQ
                     TALSSIGQFDVRVTPLQMAKVVAAVANGGMVVRPHLVREVHDYTGAILETIAPPPDGQ
                     AFSAETAGALKAMMIDVVENGTGRPVRVPGIEIGGKTGTAETGAQGKPPHSWFVAFAP
                     GIAIAVVVENGGDLGDEATGGRVAGPIAKALIEQMARRGAQSG"
     gene            11307..>11821
                     /locus_tag="VM841_10075"
     CDS             11307..>11821
                     /locus_tag="VM841_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVL90565.1"
                     /translation="MAMTVLGGRYEISDRLGVGGMAEVYLGKDRVLGRTIAVKTLLVQ
                     FAHDPSFIARFRREATSAAALNHPNIVGVYDSGSDDGRHYIVMEYVEGRTLKDTIREE
                     GPLLPERAAEIAADVCSALQFAHAHGIIHRDVKPANIMMNTRGEVKVADFGIARAASG
                     ETVTQTATVLGT"
BASE COUNT         1750 a         4246 c         4033 g         1792 t
ORIGIN      
        1 ggggggtgcg ggggagtgcg ggttaggggc tcaggatgat gcgcacgggg tcgccgcgtt
       61 ttgttgccag gatctcgacg gcgtcggcgg cctgctcaag cggcatgttt gccgtgatcg
      121 aggccgacag gtccaaccgg ccggatgcga cgagggacac cagcgtctcg atatgggtgc
      181 ggcgatagcc gtatgcgccg cgcagctgcg agtgcttgag cgcgaacagg atgctcgggc
      241 cgagcgagat cttctcgccg ccgacaccga cgatcaccgc ccggccgcct cgcgccagcg
      301 acgacacgca ggctttcacg acggcgttgg cgccgacgaa gtcgaaggcc gcgtccacac
      361 cctcgccgcc ggtggcatcg cggatcgccc cgaccgcgcc gtccggatcg accgcgatgt
      421 ccgcgcccag cgccgtcgcg cgtgaccggg cgctctgacg cggatcgacg gccgcgacga
      481 acgacgcgcc gcacaaccgc gcgatctgca ccgcgtgcgt ccccagcccg ccgacgccga
      541 agatcgcgac gcggtcgccc ggccggacct gaccggtctc gaccacggcc gcgtacggcg
      601 tagcgaccgc atcggccagg atggcgccct gatcgaacgg gatcgacttc ggcaacgcga
      661 cgcacgagga ggccggcacc acgacgtagt cggcccacgc cccgtcgaag tccacgccca
      721 tcgtgcggac gaacaggcac tcctccaggc cgtggcccgc acggcacggc cggcacgcgc
      781 cgcatcgctt gcccgcgtgc atcacgacgc gatcgcccgg cgcgggccat agcacgccct
      841 caccggccgc ctcgacggtg cccgacggtt cgtggcccat cgtgaccggc agcggcggca
      901 tcgccggaag cgaaccgtcc agcacgtgca catccgacgc gcaaaccccg cacgcggcga
      961 cccgaacgag cacctcgccg ggcccgggag ccggaaccgg aacttcgtgg atctcgaccg
     1021 gcttgccgac gccggtcatg cgcgcggcgc gcatgccgct cacccccggg ccgccttgag
     1081 cgtctcctcc ggcccggcgt agccgtcgcc gccgcggaac agcccgaccg caggcagcgt
     1141 cgcgccggcc gcccggtact cgtcgagctt gtcgcggacc gcggccgcgt cgccgatcgc
     1201 cgcgaactcc gtcagcatcg cctcgctcat cgccgcgagc gcacccggga agtccttcgc
     1261 cgcctgcgcc ttgtcgaacg cgtcgatctc ggcctggtag cccgcggccg cgatcgcgcg
     1321 ccgatagaac ggcagcgtca tgtacacgaa aaagtcgccg cgcagcgcat cgaacgccgc
     1381 ctgcttgtcc gtggtgaccg ccgtcggaac ggcggcgacg atggtcggaa ccggacgccc
     1441 ggccttctcg gccgccgccc ggaccgtcgg cgccaccgtc gaggcgatgt aggacggcat
     1501 gcagccccac agcaccaccc cgtcgcacgc ctcgcccgcg aacgacagca tcttgggcgc
     1561 cagcgcgctc agatagatcg gcagatcctt gcgcgccccg tagccgacga agccgaactg
     1621 cgtgttgtag aactcgcctt tgaacgtcac gccgtcggtg gtgaagaccg agcgaaggat
     1681 ctgcacgtac tccctcatct gcgacagcgg cttggccatc gagatgtcgt accagccctc
     1741 catcgtcacc ttgtgcgacg gccccacgcc aaggatcaga cggccgtcgc tcatctcgtc
     1801 cagcgtcgcc gcttcgatcg ccatcgcgag cggatgccgc ggaaatgccg gcaccacccc
     1861 ggtcccgagc cggatccgct tcgtggcagc ggcatacgcg gccagcgtca tcagcccgtc
     1921 gcggccggtc gtgtgggtgg aaaacaccgc ttcgtagccg agcgactcgg cgagctgcac
     1981 acgcgagacc gcgtcgccga gcgacttgcc gccggagatg aacgccgcga gcgacaccac
     2041 tacagtccga gcgtcttcgc gatgttgctg cgcaggatct cgctggtgcc ctcgtagaga
     2101 cgcatcgcgc gcacctcccg ccagtagcgc tcgattccca tctcggtcat gaagccgttg
     2161 ccgccgtaga cctggatcgc gcggtcggcg acgcggccga ccatctcggt cgccaccacc
     2221 ttggcctgcg cggcctcgcg ccggccgtcg gcgccgttgt cgatctcgtc ggccttcttg
     2281 tagaccagca gacgagcctg ctcgagctcg ttgagcgagt ccacgatcat gccctgcacg
     2341 tactggtacg agccgatcgt ggagccgaac gccacgcgcg acttcgcgta gtcgagcatc
     2401 ttgtcggtca gcagctgcgc gtagcccacg gcctgcgagg cgatcgacag gcgtccgccg
     2461 ttgatccagg ccatcgcggc cgcgaagccg tagccctctt gacccaagat gttttcggac
     2521 gtgacctcga cgtcgttgaa gaccagctcg gccggcttat ccacggtgga cgcggtgtgc
     2581 tggcggcgtg cgacggtgaa gccgggcgtg tccttgtcca ccaggaacgc ggtgatcccg
     2641 ccacgcgaac gcttctcggc gtcggtgacc gcgaacacca gcgcgaagtc ggcttcctcg
     2701 ccgtgggtga tgaaatgctt cgtgccgttg atgacgtagc tgtcgccctt gcgctccgcc
     2761 ttggtcttga tgcgcgtggc gtccgagccg gcgcccggct cggtcagcgc gaagcacatc
     2821 tcgcgctcgg cgcgcatgag cgggtccagc caccgctcgc gctgctcggg cgtgcacgcg
     2881 tgcagcaccg gcgtcggccc ttcgacgctc gccatcacgc cgccgcggcc cgccaggaac
     2941 gatccggatg ccgcgatggc ctcgtgcatg aggcactggc ccagatacga cagcccggct
     3001 ccgccgacct cctcgttcat gtgcagcgtg tagaagccga gctcggccga gcgcttgcgg
     3061 atcgcgcgct tctcctcttc cgcgctggcg atcgtgcccg tctcctgcag ctcttgcgaa
     3121 tacttcgctt cgacggggcg gatctcgcgg tcgatgaagt ccaggatgga cgtgcggagc
     3181 tcgagaagct cttgaggaat ggcgatgatg ctcacggtga cctccttgaa ggaacgccgg
     3241 tgagaatcct cgcccccggc aaccgcgaat cgtaccgttg tgaatccgcg gacgcgaagc
     3301 ggcggcccgc cggccgggcg cgcggcggcc cgtcagccgg tggcgcggcg gcccgtcagc
     3361 cggtggcgac gcgggcggcc aggcgaagcg cgcgctcgat gcgagcccga aggcggtcct
     3421 cgtcgaacgg cttgacgatg tagtcgtcca cgccggcgcg cagcccctcg atctcacccg
     3481 agtcgtcgcc ggtcagcatg atcagcggga tcatgctcgt cgccgccgag ccgcgaagcc
     3541 gcttcgcgac ctccaggcct ccgatgccgg gcatgtgcag gtcgagcagg atcagatcgg
     3601 ggcgctccac ggccgctcgc cggatcgcgt cctcgccgga ggacgcgtcg agcacctcga
     3661 agtcgtcgtc gaacatcgcg tgcgcgagct ggatcaggct cgggtcatcg tcgacgaaca
     3721 gcacccgtgc gcgaccgggg gtgtgcgggt tcgcggcggc cggcgctgcc gggtccagct
     3781 cggcgcggca gtagggacag atgttccaat ccgcggcgac ctcccggccg cacgagccgc
     3841 acgacgcggg gccgaggttg agctggcagt acgggcagac cacgaacgcg ggatcgacct
     3901 cgttgcggca cgagggacat cgcgggcggt ccggctgacg ctcgatctgc acctcgcgct
     3961 ggacctcgtc gagggtggtt atcccggctc gcaccttctc gagcccggct tcgagcaggc
     4021 tgcggacacc ggccgcgcgc gcgcccgcgc tcagcacgcc ctccgccgcc tgcgcgttga
     4081 tctgctcgcg catctgccgt cgaatcggca acacctcgaa gatgccggtg cggcccaaga
     4141 agccggtgaa cccgcacgtc tcgcatccgg ctccgcgata gagcgtcacg cccgcgagcg
     4201 tgtccggggc gaggccgagg cgcttgagcg cggcctcgga tggcggtgcc tcctgcttgc
     4261 agtgcggaca gatcacgcgc acgaggcgct gcgcgacgat caggctgagc gcttgcgcga
     4321 ccaggaacga ctcgacgccc atgtccacca aacgcgtgac cgccgaggcg gcatcgttgg
     4381 tgtgcacggt cgaaaagacg aggtgaccgg tcatcgcggc ctgcagcgag atgtgcgcgg
     4441 tctcgacgtc gcgaatctcg ccgaccatga tcacgttggg atcctgccga agaaccgacc
     4501 ggaggccgcg cgcgaaggac agccccgtct tgtcgtcgat ctgcatctgg ctgatcccgc
     4561 ggatctggta ctcgatcgga tcctcgagcg tgacgatgtt cacctcgggg gacttcttct
     4621 cgagcagggt cgcgtacatc gaagtcgtct tgccggatcc ggttgggccc gtgaaaagca
     4681 cgaagccttg tggctgggac agcgcgtcac gcacgagcgc aagctgatcg agggtcatcc
     4741 cggtctcttc gagcgagacg gtctcctcgc cctggcggag caggcggagc acgaccttct
     4801 cgccgcgcat cgtcgggatc gtggagacgc gcagcgacac gtcgctgccg gagaccacga
     4861 cccgggcgcg gccgtcctgc gggcgccggc gctcggcgat gtccatcgtg gccatgatct
     4921 tgagccgcga gaccagcgcc gcgtgaaccg tcttgggcag cgtcatcacc tcgcgcagca
     4981 gcccgtcgat tcggtagcgg acggtcacct cgctctgctg cggttcgatg tgcacgtccg
     5041 tcgcgcgggc acgcacgccg tcggcgagga tggcgttggc cagccgcacg atcggcgcgg
     5101 cttgtcccga gctcgccagg tcttcgaccg gctcttcggg ggccgtggcc accacgacct
     5161 cgacgtcggc ggcctcgccg agacggttca ccacgtcggt cgtcgcggag tccagcgaat
     5221 agaagcggtc gatcgcgtcg cgcacggccg actgggtcgc gaccgccgcc cggatcgttc
     5281 ccgcccgggc cgtcacccgc acgtcgtcga gcgcgaccac gtcggtcggg tcggccatcg
     5341 cgatcaggat cccggcctcg gtccggtcca ccggcaccac gccgagccgg tccgcggtgc
     5401 gccgcgggat gagctggagc aggtcgggat cgaccgcgac cgaggtcaga tcgacgaacg
     5461 ggatgcccag ctgcatgctg agcgcgtgcg cgagcccctc ttcgctgacc acccccatgt
     5521 ccagcagcac cgtcccgagc cggacccggc gaccggcggc gtcttccccc tgccgctgat
     5581 ggaccaaggc cgcgtcgagg acgtcctgcg tgatgagtcc ggcttcgagg agtacctcac
     5641 cgaggcgttg ggatttgggc atcgacgctc atgctaccgg cgcgaatgcc ggatccgata
     5701 gggcgcggga gaaggcgagc cgtcctccgg ccgcgcgagc gcggcacgga ggtcggatcg
     5761 gagtgggcga ccggggattt gaaccccgac cctgttgcca ggacaggaac ctgaatcctg
     5821 cgcgtctacc aagttccgcc agtcgcccgt gcaggcccgg catggtagca aacggccgcc
     5881 ggtgggggcc ggagaatgac cgtgcccgtt tccggggata ttgcttgcgc aggtcggtcc
     5941 gcgaagcgcg cgtccggcga gcaagacact agactgaccg gggagggctc ttgggcattc
     6001 tcaaggactt cgaacgccgt ctcgagcagg ccgtcgaggg ctttttcgca cgcgccctgc
     6061 ccggcggagg gctccagccg gtcgagctcg gcaagcgcat ggtccgcgcg atggacgacg
     6121 ccgccagcgt cggcgtggcc ggcgacgtca tcgccccgaa cgctttcgtc ttccggctgg
     6181 ccccgcccga tcacgagcgg ctctccggcc tcggagccac gctggagcgc gagctcgtcg
     6241 ccgtcgcccg ccgcgccgcg accgccgaag gctgggtgct gctcggcccg ccggtgatcg
     6301 agctcgtcgc cgatcccgac atgacgcccg gaacgttcat gatcgctacg gccgtcgagg
     6361 aaagcgacga ccccgccgcc ggcgccggag cgcacacgca actgatccaa atcgccgtcg
     6421 cgtccgacgc cgagctggtc gtgctgggct ccaagcgccg tgcgtatccg ttgtcgaagg
     6481 atacgctcgt aatcggccgt ctcgacgagt gcgacatcac cctgtccgat gcgggcgcgt
     6541 cgcggcggca cgccgaggtg cggcgcgagg gagacgaatg gtacgtggta gacctggact
     6601 cgacgaacgg aacgttgctg aacggcaaga gcgtgcgccg tgcgcgcctg aacccgaagg
     6661 acgtcatcct gatcggcaac acgcaagtcg agtaccgcgc ctgaagccga aaggacgacc
     6721 gtgcccgaat tggttctgac ctcgctcaag tttctctttc tggcgctgct gtacctgttc
     6781 gtggcgcgta ccgtgcgggt gatctggctc gacatcgccg gccccgccac cggcggccgc
     6841 cggcaagccg tcgctcaggc gccgcgcgcg cggcccgcgg cacgcggcgg gcgcggggcg
     6901 gtgccgcggt cgatcgcagt ggtcgaggag aacaagagcc cgaaggtgct gccgctcgac
     6961 caggatgcga tcacgctcgg gcgcgccgcc gagtgctcgg tcgcgctgga cgacacctac
     7021 gtgtcgcagc tgcacaccaa gatctttcgc aaggacggcg cctggttcgt catggacctg
     7081 gggtccacca acggcaccta tctgaaccgg atgaaggtct cggatccgat gccggtctcc
     7141 tccggcgacg aggtgcgtct cggcaagacc gtcgttgagg tgcgcgccaa gtgagccgca
     7201 ccgcaggtct gatccggtgg gccgccgcga ccgacgtggg ccgcgcgcgt cagctcaacg
     7261 aggactcctg gttcggcggc gagcggatct acgcggtcgc cgacgggctc ggcggccacc
     7321 gggccggcga ggtcgcctcg cgcatggccg tcgaggagct cgcccggctc gaccgcaagc
     7381 cgccggacgg cgacatcggc gccgagctcg tcgaagcggt gcgcgccgcg ggccgtgcgg
     7441 tcgcgcagcg ctccgcggga gatcccgaga ccgccggcat ggccacgacc gtcaccgcga
     7501 tcgcgatgga cggcggagcc gcgcacctcg cgcacgtcgg cgactcgcgc tgctatctgc
     7561 tgcgcggcga gcagatgacg ctggtctcca ccgatcacac gctggtcgcc cgcatggtgg
     7621 acgagggcgc catcacgctc gagcaggccg agacccatcc gcagcgaagc gtcctgaccc
     7681 gcgcgctggg caccgacccc gaggtcgacg tcgaccatca ggtgatcccc ctcgcaccag
     7741 gcgaccggat tctgctgtgc tccgacgggc tctcctcggt cgtgcccgag cccgagatcg
     7801 ccggcctgct ggcgtcggtg cgggacggcg acgatctgtg ccgcgcgctc gtcgacgagg
     7861 cgaatcgccg cggcgggccg gacaacatca cggtgctgat catcgacgtg cccgccgacg
     7921 cgcagcttcc cgtctacgac gggacctcct cgatggcggc gctgcccgag gagccggtcc
     7981 gccggccggt tgcaccgccg cccgcgcgcc gtacgcgccg cgttccggtc cggctgatcg
     8041 tgtggctctc gatcctggtc ctggccgtgg cgggcggtct gggcggcctg cgcatctggt
     8101 cggaccggaa ctggttcgtg ggcttcgacg gcgaccaggt cgcgatctac cggggactgc
     8161 ccaccgactt ttgggtctcg ctccaccgcg tgcacgagcc gaccgcgctc acccgcgatc
     8221 aggtcgagcc gcgctacgtg gagcggctcg aggaaggcct tcgtgcgtcc acgctggagg
     8281 aggcacgcga gatcgcctcg cggatcccgc gtgcgcccac gcccacgccg tcgcccacgc
     8341 cgtcgcccac gcggtcgccc tccgtgctgc cctcgccgtc accgctcggg agcccgacgt
     8401 gaaccgccga agcgccgagg cgggcctgat cgcgatggcg gtggccatct cgctcggggc
     8461 cttgctgctg gtcgccttcg cccagtccgc cgaggtgccc ccggacttct tcgggcatgc
     8521 cgtcttcgtg gccgccttcg ccgtcggcat gcacctgatc gtgcgcaagg cggcgccgtc
     8581 cgccgacccg gtcctgctcg cctgcgcgat cgcgctgacg ggcgtgggct atgccgtgat
     8641 cgagcgcttg gacccggacc ttgcggtttc gcaggcgctg tggctcgcga tcggcggggc
     8701 cgcgttcgcc gcgacgcttc tgctgctgcg cgatcaccgc acgctcgaga agttccgcta
     8761 ttcgctgatg ctgctcggcg tggcgttgct gctgctgccg ctcgcgccgg tgatcggcaa
     8821 gaccgtccgc ggcgcgcgtc tgtgggtcgc ggtcgggccg atgaccttcc agcccgccga
     8881 ggccgccaag atcgtgctcg ccgcgttcct cgccggctat ctcgcctcca agcgggaggt
     8941 catgaccgtg ccgaccgcgc gcattgggcc gctcaagctt ccggcgccgc ggcatttcgg
     9001 gccgctcatc atcgcgtggg tgctgtcgct ggcgatcatg gtcttccagc gcgatctcgg
     9061 ctcgagcgtg ctgttcctcg cgctctacat cgtcacgatc tacgtcgcga cctcgcgcgc
     9121 gtcctacgtg gttgccggcg tcgggctgct cgcgggcggc atcgcgttcg catcgatggc
     9181 gttctcgcac gtggccagcc gcttcagcgt ctggctcgat ccgtggtccg acatcgacgg
     9241 gaagggcttt cagatcgccc agtcgctgtt cgcgctcggc accggcggcg tcgccggcga
     9301 gggcctcggc cgcggccggc ccgacttcat cggcaagtcc tcgatcgtgg cgaccgactc
     9361 gatcttcgcc gcgatcggcg aagagctcgg gctgatcggc accgtcggaa tcctgatgct
     9421 gttcgcggtc atggtcgcgc gtggcttcca catcgcgctt cgctcgcgcg actccttcgg
     9481 ggcgttgctc gcgaccggcc tcaccgcgat catcggtgtg cagacgttcc tgatcgtcgg
     9541 cggcgtcacg cgcttgatcc cgctgaccgg gatcacgctg ccgttcgtca gctacggcgg
     9601 gtccagcatc gtggccaact tcgtgctcat cgcgatcctg atgcggatca gcaacatcga
     9661 ggcgggcacc cacgccgaag cggccgaggc gcgtgggggt gcccgggcgg gcggcgcggc
     9721 cgtagccacg acggacgtgc agggccgcga cgaggcaccg ctcgcggacc tgacctcggt
     9781 gcaccgcatc gacgtacctc cggagaatcg ctcgtgaacc gctcgatccg tcgcgtcgcg
     9841 ctcacgctcg gagtcgcttt cctggccctg ctggtgcaac tcaactacga ccaggtcgtg
     9901 gcgtccaagc gcttcgccga gcatccggcg aatcggaggc tgctggtcaa ggagtactcg
     9961 acgcagcgcg gtgacatcgt cgcgtccggt cagatcgtgg cccgcagcgt cgcaaccgac
    10021 gacaagctca agtacctgcg cgagtacccg ctcgccgcgc tcactgccga gatcagcggc
    10081 tactactcgc tcgtctacgg cggcgcgggc atcgagcgcg cctacaactc gtacctgacg
    10141 ggcaacaagc cggcgcgggt cgagtcgatc gtggaccaac tgctcggccg ggaccgcaaa
    10201 ggaaactcgc tgatgctcac gctcgatctg gagctgcaga aggtcgcggc cgagaagctc
    10261 ggcaagcagc gcggtgcggt cgccgcgatc gatccgcaga cgggcgcggt gctggcgctg
    10321 tactccagcc ccacctacga cccgaacgcg ttgtcgagcc acaacaccgg cgcgatccgc
    10381 cgctcgtggg agttctacca aaacgccgag cacaacccgc tgctgtcccg ctcgttccag
    10441 gagcgctacc cgccgggctc gacgttcaag gtcctcgtgg cggccgccgc gctcgaaaac
    10501 ggcatcaaac ccgagaccgc ataccccaac ccgcgtgcct ttccgttgcc gctgacgaac
    10561 cgcgagctcg gcaacttcgg cggcggcgcc tgccgcgggg gctccggcgg cacgatcacc
    10621 atggcccagg gccttcgcgt atcgtgcaac accaccttcg cccagatcgg cctgcagatc
    10681 ggcgccgaca agctcgtcgc ccaagccgag aagctcggct tccagcaaag gcccgtcttc
    10741 gacctgcccg ccgtgccctc gtgcatccgc accgccgcac gcggctgcgc cgacccggac
    10801 ctcgacgcgc cccagaccgc gctctcctcg atcggacagt tcgacgttcg cgtcacgccc
    10861 ttgcagatgg ccaaggtcgt ggccgcggtc gccaacggcg gcatggtcgt gcggccgcat
    10921 ttggtgcgtg aggtgcatga ctacaccggc gcgatcctcg agacgatcgc gccgccgccg
    10981 gacggccagg ccttctcggc cgagaccgcc ggcgcgctca aagcgatgat gatcgacgtc
    11041 gtcgagaacg gcaccggccg gccggtgcgt gtgcccggca tcgagatcgg cggcaagacc
    11101 ggcacggccg agaccggcgc ccagggaaag ccgccgcact cgtggttcgt ggcttttgcc
    11161 ccggggatcg cgatcgcggt cgtggtcgag aacggcggag acctcggcga cgaagcgacc
    11221 ggtggccgcg tggccggccc gatcgcgaaa gcattgatcg aacagatggc ccggcgtggg
    11281 gcccaatccg gttaaggtag cgctcgatgg cgatgacggt gctcggcggg cgatacgaga
    11341 tcagcgaccg gctcggggtc ggcggcatgg ccgaggtata tctcggcaaa gaccgcgtcc
    11401 tcggccgcac catcgcggtc aagaccttgc tcgtgcagtt cgcgcacgac ccgtcgttca
    11461 tcgcgcggtt tcgccgcgaa gcgaccagcg ccgcggcgtt gaaccatccc aacatcgtcg
    11521 gcgtctacga ctccggcagc gacgacggcc gccactacat cgtgatggag tacgtcgagg
    11581 gccgcacgct caaagacacg atccgcgagg aaggtccgct gctgcccgag cgcgcggccg
    11641 agatcgccgc cgacgtgtgc agcgccctgc agttcgcgca cgcgcacggc atcattcacc
    11701 gcgacgtcaa gcccgccaac atcatgatga acacgcgagg cgaggtgaag gtcgccgact
    11761 tcggcatcgc acgggccgcc tccggcgaga ccgtcacgca gaccgcgacg gtactcggca
    11821 c
//
LOCUS       DATLFO010000248         7195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_114816, whole genome shotgun sequence.
ACCESSION   DATLFO010000248 DATLFO010000000
VERSION     DATLFO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114816"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..714
                     /locus_tag="VM841_10080"
     CDS             112..714
                     /locus_tag="VM841_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90566.1"
                     /translation="MVRRALLAGALALALTVSGHAAGAHVVDPCASTTCVFWQDHQGN
                     CHKAQKVEISDGTNGGGYTRTHAITQKAYSGPPGPNVQCHVSWYLLAGKHKWQHVVWK
                     QVYVPRYGEYVDAVCYDSGWRTNTVNDWKVIETHDWGKTNGGAPCGPGKYFLSFKASI
                     WIDWINQGKGAWTADQFVIYAPDPYPAANGTVQHNYHSWN"
     gene            723..1010
                     /locus_tag="VM841_10085"
     CDS             723..1010
                     /locus_tag="VM841_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90567.1"
                     /translation="MKTIRIAATAVATLLLIALIVGQPSAAPPTRPVYVGVQPGAVPP
                     VTHRVDGTGRIVTDASGQPAVFPNPDRRPVTELPVMGPPAYCPKNVDCRPQ"
     gene            complement(1011..1790)
                     /locus_tag="VM841_10090"
     CDS             complement(1011..1790)
                     /locus_tag="VM841_10090"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HVL90568.1"
                     /translation="MLLAVDVGNTQTVIGVFERDQLEQHWRISSQADRTPDELALIFQ
                     GLLVQAGLSFSKQVTGVVISSVVPRMTQALREMTERYFRFPPVVVGPGIKTGLAIMTD
                     NPKEVGADRVVNAVAAHEMFGGPCIVIDFGTATTVDAVSAKGEYLGGAIAPGVEISTN
                     ALVSRGAQLKRVEFTVPKHVAGKSTDEAIRSGVIYGFAGQVDAIVQRMKEEMGGDPTV
                     VATGGLAAVITPHMRVAVKNEPWLTLVGLRIVFDRNAHTHE"
     gene            complement(1802..2626)
                     /gene="nadC"
                     /locus_tag="VM841_10095"
     CDS             complement(1802..2626)
                     /gene="nadC"
                     /locus_tag="VM841_10095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HVL90569.1"
                     /translation="MKPDPGAVEEAVRCALAEDIGSGDVTTNSIVDPAGAGMGIVEAR
                     QDCVVAGVDAARETFRQIGGCDFPGDLADGDRLKEGDRVPLVIGSLRSILTGERVALN
                     FLQRLSGVATLARRFVDAAPGVQIRDTRKTTPGLRVLEKAAVAAGGGVNHRFGLFDAI
                     LIKDNHIAVAGSIAEAIRRARTAGMVVQVECDTIAQVREALAEGAEAILLDNMSPGML
                     REAVGIIDCAAFVEASGGITFDSVAAVAATGVDAISVGALTHSAPAIDLSLDVRPE"
     gene            complement(2632..3036)
                     /gene="panD"
                     /locus_tag="VM841_10100"
     CDS             complement(2632..3036)
                     /gene="panD"
                     /locus_tag="VM841_10100"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HVL90570.1"
                     /translation="MFRTMLKSKIHRATVTEANVNYVGSITVDEDLMKAADMLEYERV
                     QVVDVDNGARLETYVIPGPAGSGVICLNGAAARLVTPGDKVIIMSYAMLADAEARNMK
                     PTVVFVDEANRITHMGTSVTELGIDVSAFDLA"
     gene            complement(3041..3910)
                     /gene="panC"
                     /locus_tag="VM841_10105"
     CDS             complement(3041..3910)
                     /gene="panC"
                     /locus_tag="VM841_10105"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HVL90571.1"
                     /translation="MRLVETKAALRALLDADRRNGKRVGFVPTMGYLHEGHAGLIRAA
                     RAECDVVVVSIFVNPLQFGANEDLGAYPRDLDRDLAVCKAEGADYVLHPTVDEMYGKD
                     STLRITVGPIGDVLCGASRPGHFDGVATVVAKLFNIAGPCRAYFGEKDAQQLVVIRRL
                     ADTLDFPVEIVGCATVREPDGLAMSSRNTYLKEDERTAAAVLSRALFEARDAIERQGE
                     RDGAKVARLIAERISSEPLATLDYAACVNPHTLEDVSEITGRVLLAAAARIGNARLID
                     NTTATAPAAPSGG"
     gene            complement(3907..4794)
                     /locus_tag="VM841_10110"
     CDS             complement(3907..4794)
                     /locus_tag="VM841_10110"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HVL90572.1"
                     /translation="MTEFARPSRNDGGVSSSQAPGLTEVAIVGAGRVGCSMGRALARA
                     GHRIVAASTAAPASATRVRDHLGNVPVTDPHDAALAADLIVIAVPDDALSAVATDVAR
                     GLRPHATVIHTSGIHGLDVLAPCGERIAAIHPAQAIPRHDTDLTGVFFGVTASDAVKD
                     HAHAIVADLSGIAIDVSEDRRTLYHAALCMASNFAVTLAGDAADLLPDAGVLAPLLRG
                     TVDNIIAMGADAALTGPVVRGDAGTVRAHIGALPPHLLDSYVANVRRTLDRAVQSGRL
                     DPHKAAAVEAALREAKALR"
     gene            complement(5175..5672)
                     /gene="folK"
                     /locus_tag="VM841_10115"
     CDS             complement(5175..5672)
                     /gene="folK"
                     /locus_tag="VM841_10115"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HVL90573.1"
                     /translation="MRAYLALGANLGDRAATLASVPAALLGRSIFVTNASRVFETTPV
                     GGPDDQPDYLNQVLEIQTMCSPREVLDACRAVEFAHGRERDRRGEPKNEPRTLDIDFL
                     AIDGGETIDDHDLIVPHPRLHERAFVLVPLAEIAPDLEIPGHGTVTALLVGLNDTDGV
                     RPVQP"
     gene            complement(5675..6055)
                     /gene="folB"
                     /locus_tag="VM841_10120"
     CDS             complement(5675..6055)
                     /gene="folB"
                     /locus_tag="VM841_10120"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HVL90574.1"
                     /translation="MEGTDLIEVRGLKLKGRHGVTDEERADAQAIEVSLAIRLDTREA
                     ATFDDINATLDYAEAVTQVTKIVEGESFQLIETLADHIARQILSNKRALDVWVRVSKP
                     DVNLPVDEVAVEISRSREDMDDLR"
     gene            complement(6063..6902)
                     /gene="folP"
                     /locus_tag="VM841_10125"
     CDS             complement(6063..6902)
                     /gene="folP"
                     /locus_tag="VM841_10125"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVL90575.1"
                     /translation="MNDWQLMRGALLLSQRTHVMGIVNVTPDSFSDGGAFFDEQAAID
                     HGLRLADEGADILDVGGESTRPGADPVGEDEEIRRTVGVVEALAVKTGLPVSIDTTKA
                     AVARAALDAGAQIVNDVAAGRLDDALLPLAAERGAAVVLMHMQGSPRTMQQDPTYDDV
                     VTDVRDFLIQRAEAAIAAGVARDKIAIDPGVGFGKTLQHNLALLNRLDEICATGYPVL
                     VGTSRKSFIGAVLDGAPVTERMEGTAATVALAIARGSRLVRVHDVRAMVKVARVTDAI
                     VNG"
     gene            complement(6899..>7195)
                     /locus_tag="VM841_10130"
     CDS             complement(6899..>7195)
                     /locus_tag="VM841_10130"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HVL90576.1"
                     /translation="VAYLPGEDGRICGISKLTRLIDVLGKRLQVQERLVAQAADAIEK
                     ALGARAVLVYAEAEHLCMTMRGARAEGSRIVTTEARGLWAAGAAARTEILSLIR"
BASE COUNT         1110 a         2385 c         2541 g         1159 t
ORIGIN      
        1 catcgttgtc gggcacttcg tcggactgga ctctcggtgt cgccggtcct agcgtcgccg
       61 ccacgttgat gcgcagccgc gggcgctgcg caggcatggg aggtgatggg aatggttcga
      121 cgagcccttc tcgcgggcgc gcttgcgctc gcgctgactg tttccgggca cgctgccgga
      181 gcgcacgtgg tcgacccgtg cgcatcaacc acgtgcgtgt tctggcaaga ccatcaaggg
      241 aactgtcaca aggcgcagaa ggtcgagatc agcgacggga ccaacggtgg cggctacacc
      301 aggacgcacg cgatcacgca gaaggcatac agcggcccac ccggaccgaa cgtgcagtgc
      361 cacgtgtcgt ggtatctgct ggccggcaag cacaagtggc agcacgtggt ctggaagcag
      421 gtctacgtgc ctcggtacgg cgagtacgtg gacgcggtct gctacgactc gggctggcgt
      481 accaacaccg tcaacgactg gaaggtcatc gagacgcacg actggggcaa gacgaacgga
      541 ggcgctccat gcgggccggg caagtacttc ctttcgttca aagcctccat ctggatcgac
      601 tggatcaacc aaggaaaggg tgcttggacc gccgaccagt tcgtgatcta cgcgcccgat
      661 ccgtacccgg ccgcgaacgg cacggtgcag cacaactatc actcatggaa ttaggaggag
      721 ccatgaagac gattcgaatc gcggccacgg ccgtcgcgac gctcctgctg atcgcgttga
      781 tcgtcggaca gccgtccgcc gcgcctccca cgcggccggt ctacgtcggg gtgcagccgg
      841 gtgccgtgcc cccggtcacg catcgcgtgg acggcaccgg gagaatcgtt accgacgcat
      901 cggggcagcc cgcggtgttc ccgaacccgg atcggcggcc ggtcacggag ctgccggtca
      961 tgggcccacc cgcctactgc ccgaagaacg tggactgccg gccgcagtag ctactcgtgg
     1021 gtgtgcgcgt tgcgatcgaa gacgatgcgc aggccgacga gcgtgagcca cggctcgttc
     1081 ttcacggcga cgcgcatgtg cggggtgatg acggcggcga gcccaccggt cgcgacgacg
     1141 gtggggtcgc cgcccatctc ttccttcatg cgctgcacga tcgcatcgac ctgaccggca
     1201 aagccgtaga tgacaccgct ccggatcgcc tcgtcggtgg acttgcccgc gacgtgcttc
     1261 ggcacggtga actcgacgcg cttgagctgc gcgccgcggg acaccagcgc gttggtcgag
     1321 atctcgacgc ccggcgcgat cgcgccgccg agatactcgc ctttggcgct gaccgcgtcg
     1381 acggtggtgg cggtgccgaa gtcgatgacg atgcaggggc cgccgaacat ctcgtgtgcg
     1441 gcgacggcgt tgacgacacg gtcggcgccg acctctttcg gattgtcggt catgatcgcc
     1501 aggccggtct tgatgccggg accgacgacg acgggcggga agcggaagta gcgttcggtc
     1561 atctcgcgca gcgcctgcgt catgcgcggc accacgctgg agatcacgac accggtcacc
     1621 tgcttggaaa acgacaggcc cgcttggacc agcagccctt ggaagatcag cgccagctcg
     1681 tcgggcgtgc gatcggcttg cgaggagatg cgccaatgct gctcgagctg gtcgcgctcg
     1741 aacaccccga tcacggtttg cgtgtttccg acgtccacgg ccagcagcat ctgcgtctct
     1801 cctactcggg gcggacgtcc agggacaagt cgatcgccgg cgcggaatgg gtgagcgcgc
     1861 cgacgctgat cgcgtccacg ccggtcgcgg cgacggcggc gacgctgtcg aaggtgatgc
     1921 cgccggaggc ttcgacgaag gcggcacagt cgatgatgcc gacggcctcg cgcagcatgc
     1981 cggggctcat gttgtcgagc aggatggcct ccgcgccttc ggcaagagct tcgcgcacct
     2041 gcgcgatggt gtcgcactcg acctgcacga ccatgccggc ggtgcgcgcg cgacgaatcg
     2101 cctcggcaat cgagcccgcg acggcgatgt ggttgtcctt gatcaggatc gcgtcgaaca
     2161 ggccgaagcg atggttcacg ccgccgcccg cggcgacggc ggctttctcc agaacgcgga
     2221 ggccgggcgt ggtcttgcgg gtgtcgcgaa tctgcacgcc gggagccgcg tccacaaaac
     2281 ggcgcgcgag ggtcgcgacg cccgacaggc gctgaagaaa gttgagcgcg acacgctcgc
     2341 ccgtcaggat cgaacgcagc gagccgatca ccagcggcac gcgatcgcct tccttcagcc
     2401 ggtcgccgtc ggcgaggtcg ccgggaaagt cgcagccgcc gatctgccgg aaggtttcgc
     2461 gcgcggcatc cacaccggcg accacacaat cctgccgcgc ctcgacgatg cccatgccgg
     2521 ccccggccgg atcgacgatc gagttcgtgg tgacgtcgcc gctgccgatg tcttcggcca
     2581 gtgcgcaccg aaccgcctct tcgacggcgc caggatcggg cttcatcgca gctacgccag
     2641 gtcgaacgcc gacacgtcga tgccgagctc ggtgacggat gtgcccatat gggtgatgcg
     2701 gttggcctcg tcgacgaaga ccacggttgg cttcatgttg cgcgcctcgg cgtcggcgag
     2761 catcgcgtag ctcatgatga tgaccttgtc gccgggcgtg accagccgtg cggctgcgcc
     2821 gttcaagcag atgacgccgc tcccggccgg gccggggatc acgtacgtct cgagccgcgc
     2881 gccgttgtcg acgtccacga cctggacgcg ctcgtattcg agcatgtcgg cggctttcat
     2941 caggtcctcg tccaccgtga tcgagccgac gtagttgacg ttggcctcgg tgacggtggc
     3001 ccggtggatc ttggacttca gcatcgttcg gaacatgccc tcaccctccg ctcggcgccg
     3061 ccggagcggt cgccgtcgtg ttgtcgatca gtcgtgcgtt gccgatccgt gcggccgcgg
     3121 cgagcagcac ccggccggtg atctcgctta cgtcttcgag cgtgtgggga ttcacacatg
     3181 ccgcgtagtc cagcgtcgcg agcggctcgg acgatatgcg ctcggcgatg agccgggcaa
     3241 ctttggcgcc gtcgcgctcg ccctgcctct cgatcgcgtc gcgggcctcg aagagcgcgc
     3301 gcgacagcac ggcggccgcc gttcgctcgt cttccttcag gtacgtgttg cggctggaca
     3361 tcgccagacc gtcgggctcg cgcacggtcg cgcagccgac gatctcgacg ggaaagtcga
     3421 gggtgtcggc gagacggcgg atcacgacca gctgctgcgc gtccttttca ccgaagtagg
     3481 cgcggcacgg gccggcgata ttgaacagct tggcgacgac ggtggcgacg ccgtcgaagt
     3541 ggccgggccg gctggcgccg cacagcacgt cgccgatcgg gccgacggtg atgcggagcg
     3601 tcgagtcttt accgtacatc tcgtcgacgg tcgggtgcag cacgtagtcg gcgccttcgg
     3661 ccttgcagac cgcaaggtcg cgatccaggt cgcgcggata cgcgcccagg tcttcgttcg
     3721 caccgaactg cagcgggttg acgaagatgc tcacgacgac gacgtcgcat tcggcgcgcg
     3781 cggcgcggat caggccggcg tggccttcgt gcagatagcc catcgtaggc acgaagccga
     3841 cgcgtttgcc gtttcgccgg tcggcgtcca gcagcgcgcg caacgcggcc ttggtctcga
     3901 cgagcctcat cgcagcgcct tcgcttcccg cagcgcggcc tcgacggctg cggccttgtg
     3961 ggggtcgagc cggccgcttt ggaccgcgcg gtccagcgtg cgccgcacat tggcgacgta
     4021 ggagtcgagc agatgcggcg gcagcgcgcc gatgtgggcg cggaccgtgc cggcatcgcc
     4081 gcgtacgacg gggccggtca gtgcggcgtc ggcgcccatc gcgatgatgt tgtcgacggt
     4141 gccgcgcagc agcggtgcca gcacgccggc gtccggcagc agatcggcgg cgtcgccggc
     4201 gagcgtgacc gcgaagttgg atgccatgca tagcgccgcg tggtagagcg tgcggcggtc
     4261 ctcggaaacg tcgatcgcga tgccgctcag gtccgcgacg atcgcgtggg catgatcttt
     4321 gaccgcatcg gatgcggtga ctccgaagaa gacgccggtc agatcggtgt cgtggcgcgg
     4381 gatggcctgc gccggatgga tcgcggcgat ccgctcgccg cacggcgcca gcacgtcgag
     4441 gccgtggatg ccgctggtgt ggatgacagt tgcgtgcggc cgcaggccgc gcgcaacgtc
     4501 ggtggcgacg gccgaaagcg catcgtcggg gacggcgatc acgatcaggt cggcggccag
     4561 ggccgcgtcg tgcgggtcgg tgaccggcac attgcccagg tggtcacgca cgcgggttgc
     4621 ggacgcgggc gcggcggtcg aagcggcgac gatgcggtgg ccggcgcgag ccagggcgcg
     4681 gcccatcgag cagccgacac ggccggcgcc gacgatcgcg acctcggtga ggccgggcgc
     4741 ctgagaagaa gaaacccctc cgtcgtttcg ggaggggcga gcaaactcgg tcactttttc
     4801 cctccgttcc ggtcctcgtc atcgaggtac cgttcggcat cgttcactat agcgcaagcg
     4861 ggcccgccag acatcccgat cgcgcgcgcc gcagggcagg ggggaagggt tcgaacaccc
     4921 tgtgcaacca agttgcaccg gcactgcggc gggtgggcgg cgcgggggcg gctgcggcgg
     4981 cccggcgacg ggaccggccg agcggcacga cgcggtcgat cacgcctgca acttagttgc
     5041 acagggtccg ggggaaaccc tggtgtcctg gcgaggtgat gtcgagcgag cgggcgacga
     5101 gacgcggtcc ctggtcgctc gggccaggac gaagccgcgt tctgccaacg gttcggtttc
     5161 gcgacgcggc cagttcaagg ctgaacaggg cggacgccgt cggtgtcgtt caggccgacc
     5221 aacagagccg tgactgtgcc gtggccgggg atctccaggt ccggggcgat ctccgcgagc
     5281 ggcaccagga cgaaggctcg ttcgtgcaag cgggggtgcg ggacgatcag gtcgtgatcg
     5341 tcgatggtct cgccgccgtc gatggcgagg aaatcgatgt cgagcgtgcg gggctcgttc
     5401 ttcggctcgc cgcggcggtc gcgttcgcgg ccgtgggcga actcgaccgc gcggcaggcg
     5461 tcgagcacct cgcggggcga gcacatggtc tggatctcga gcacttggtt cagatagtcg
     5521 ggctgatcgt cgggtccgcc gacgggcgtg gtctcgaaca cgcgcgaggc gttggtcacg
     5581 aagatggagc ggccgagcag cgcggcaggc accgacgcga gcgtcgctgc gcgatcgccg
     5641 aggttggcgc cgagcgcgag ataggcccgc acgcctagcg gaggtcgtcc atgtcttcac
     5701 gcgaacgcga aatttcgacg gcgacctcgt ccaccggcaa gttcacgtcg ggcttggaca
     5761 cgcgcaccca cacgtcgagc gctcgcttgt tggacaggat ctgtcgcgcg atgtggtcgg
     5821 cgagcgtctc gatcaactgg aacgactcgc cttcgacgat cttggtgacc tgcgtgacgg
     5881 cttcggcata gtccaaggtc gcgttgatgt cgtcgaacgt cgctgcttcg cgcgtgtcca
     5941 gacggatggc gagcgagacc tcgatcgcct gcgcgtcggc gcgctcttcg tccgtgacgc
     6001 cgtgacggcc cttcagcttc agaccgcgaa cctcgatcag atcggtgcct tccatcgctc
     6061 ccctacccgt tgacaatcgc atcggtcacg cgtgcgactt tcaccatagc gcgaacatcg
     6121 tggactcgaa cgagcctgga gccccgcgcg atcgccagcg cgacggtggc ggccgtccct
     6181 tccatccgtt ccgtcaccgg cgccccgtcc aaaacggcgc cgatgaacga tttgcggctg
     6241 gttccgacca gcaccggata gccggtcgcg cagatctcgt ccagccggtt gagcagcgcc
     6301 agattgtgct gcagcgtctt gccgaatccg acgcccggat cgatcgcgat cttgtcacgc
     6361 gcgacgccgg ccgcaatcgc agcctccgcc cgctggatca ggaagtcgcg cacgtcggtg
     6421 acgacatcat cgtatgtcgg gtcctgctgc atggtgcgcg gcgagccttg catgtgcatc
     6481 aggaccactg cggcgccgcg ctcggccgcg agcggcagca gcgcgtcgtc gaggcggccg
     6541 gcggcgacgt cgttgacgat ctgcgcgccc gcatccagcg cggcgcgcgc aaccgcggcc
     6601 ttcgtcgtgt cgatgctgac cggcaggccg gtcttgacgg ccagcgcctc gacgacgccg
     6661 acggtgcggc ggatctcttc gtcttcgccg accggatcgg cgccaggccg cgtcgattcg
     6721 ccgccgacgt cgaggatgtc ggcgccctcg tcggccagac gcaggccgtg atcgatcgcg
     6781 gcctgctcgt cgaagaacgc gccgccgtcg ctgaacgaat ccggtgtgac gttgacgata
     6841 cccatcacgt gcgtgcgctg cgacagcagc agcgcgccgc gcatcagctg ccagtcgttc
     6901 atcggatcag cgacaggatc tcggtacgag ccgcggcgcc ggcggcccac aggccgcgcg
     6961 cctcggtcgt gacgatgcgc gatccttccg cgcgcgcgcc gcgcatcgtc atgcacagat
     7021 gctcggcttc ggcgtagacc aggacggcgc gcgcccccaa cgccttttcg atcgcgtcgg
     7081 cggcctgggc caccagacgc tcctgcacct gcagccgctt gccgagcacg tcgatcaggc
     7141 gcgtcagctt cgagatgccg cagatgcggc catcctcgcc gggcaggtac gcgac
//
LOCUS       DATLFO010000249         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_114904, whole genome shotgun sequence.
ACCESSION   DATLFO010000249 DATLFO010000000
VERSION     DATLFO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114904"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="VM841_10135"
     CDS             complement(<1..2134)
                     /locus_tag="VM841_10135"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HVL90577.1"
                     /translation="MASLGYYLLLAAFVTAAYAAAISVAGARRRSRALIESGIGAFHL
                     IAAIMAAASAVIIYSFVTGDYSIKYVQRYSDSALPMFYKLASYWGGLDGSIMFWVTLL
                     SFFGSAAVYVNRERHRELIPYVVATISVTQFFFLLLMVVHNNPFATFLTESPTDGTGL
                     NPLLQNFYMVIHPPTMYLGFVGLTIPFAFGMAALITGHLDDSWLRAVRRWTMIAWLFL
                     SIGLGLGMIWAYEELGWGGYWMWDPVENAGLLPWFTATAFLHSVMVQERRGMLRVWNV
                     SLVIVTFFLTIFGTFMTRSGVVQSVHAFGEDPELARMFTIFMVTTLVVAFGFVIYRLP
                     LLRARNELDSWVSREAAFLLNNWILLFSAFFVLFATMFPTLSEAVTGNRITVGPPFFN
                     QWMIPIGLTLLFLTGVGPLLAWRKSTLINLRDQFLIPVTCGLTVGVGVVALGVRVWSS
                     GICFALAGFVLGTIGQEMWRGARVRQQATGTDVFTAWVGLVGRNKRRYGGYIIHVAIV
                     LMFLGFAGEGFKESKIVLLMPGQEVTVRDYTVRLDAVRVTEDAQKQAQTAYITVLRDG
                     AEFTKMYPARWTFFKTPDQPTTEVAIRRGFAEDLYITVPQFDVANQAAHTEVVVTPLV
                     NWVWFGFGMLALGTLLALMPESAFAFATANVPAGAATASVLVLALALGGAVPLHAQHD
                     DTGQLSLQILTPEQRAITSKLACWCGGCP"
     gene            complement(2135..2545)
                     /locus_tag="VM841_10140"
     CDS             complement(2135..2545)
                     /locus_tag="VM841_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HVL90578.1"
                     /translation="MSNKAAKVGFTTLVLATAFGILLYTTLGESMQYYKFVDEVLAEP
                     QAWQDKNLQVHGYVVPKSIGRKRDSLEYQFDIERNGKVLRAYYTGIVPDTFKDESEVV
                     LTGRLADNGTFHATQMSAKCPSKYEEAPGSSLSK"
     gene            complement(2615..>3193)
                     /locus_tag="VM841_10145"
     CDS             complement(2615..>3193)
                     /locus_tag="VM841_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL90579.1"
                     /translation="LSDRAIPLQGNYCAAKHALKAFTDTLRMELEDEGAPISVTLVKP
                     ASIDTPFFDKAKTYLGVEPQPVPPVYAPEVVADVIIHAARRPIREIGAGGAAVMLSAA
                     RFAARAADLYMEKFTFQGQKTDKPASPDRPHNLYRAVARDGGERGSNWKGRTKESSLY
                     TRAMLHPVAAAGIFASVVGLGMWTLGKGASRH"
BASE COUNT          624 a         1058 c         1002 g          509 t
ORIGIN      
        1 gtggacatcc gccgcaccag caggccagct tgctcgtaat ggcccgctgc tccggcgtga
       61 gaatctgcag gctcaactgc cccgtatcgt cgtgctgcgc atgcagcggc accgcgcccc
      121 caagcgcaag tgcgagcacg agcaccgacg ccgtcgcagc accggccggg acattcgccg
      181 tcgcgaacgc aaacgcggat tccggcatca gcgcgagcag ggtgcccagc gcgagcatgc
      241 cgaagccgaa ccatacccag ttcacgagcg gtgtgacgac gacttcggta tgcgccgcct
      301 ggttcgcgac gtcgaactgc ggaaccgtga tgtagaggtc ctcggcgaat ccgcggcgga
      361 tcgccacttc tgtagtcggc tggtcgggcg tcttgaagaa cgtccagcgc gccgggtaca
      421 tcttcgtgaa ctcggcgcca tcgcgcagca ccgtgatgta cgcggtctgc gcctgcttct
      481 gcgcgtcttc cgtgacgcgc accgcatcga gccgcaccgt gtagtcgcgg accgtgacct
      541 cctgccccgg catcagcagg acgatcttgc tttccttgaa cccctcgccc gcgaagccga
      601 ggaacatcag aacgatcgcg acgtgaatga tgtagccgcc atagcgacgc ttgttgcggc
      661 cgaccaaccc gacccacgcg gtgaagacat cggtgccggt cgcctgctgg cgcacccgcg
      721 ccccgcgcca catctcctgc ccgatcgtgc cgagcacgaa cccggccagg gcgaaacaga
      781 tccccgagct ccacacgcgg acgccgagcg ccacgacacc aacgccaacc gtcagtccgc
      841 acgtcaccgg aatcaggaac tgatcgcgaa gattgatcaa tgtggacttc cgccacgcga
      901 gcagcggacc gacgccggtg aggaacagca gcgtgagacc gatcggaatc atccactggt
      961 tgaagaacgg cgggccgacg gtgatgcggt tcccggtcac cgcctcgctc agcgtcggga
     1021 acatcgtcgc aaacaggacg aagaacgcgc tgaacagcag aatccagttg ttgagcagga
     1081 acgcggcctc gcgcgagacc cacgagtcga gctcgttgcg cgcccgcagc agcggcaggc
     1141 ggtagatcac gaatccgaac gccaccacca gcgtggtcac catgaagatg gtgaacatcc
     1201 gcgccagctc gggatcctcg ccaaacgcgt gcaccgactg cacgacgccg gagcgggtca
     1261 tgaaggtgcc gaaaatggtc aggaaaaagg tgacgatgac gagcgagacg ttccagacgc
     1321 gcagcatgcc gcgccgctcc tggaccatca ccgagtgcag gaaggccgtg gccgtgaacc
     1381 acggcagcag ccccgcgttc tcgaccggat cccacatcca gtagccgccc cacccgagct
     1441 cttcgtaggc ccagatcatt ccgaggccca gcccgatcga gagaaacagc cacgcgatca
     1501 tcgtccagcg gcgcaccgcc cggagccagg agtcgtccag gtggccggtg atcagcgccg
     1561 ccatgccgaa cgcgaacggg atcgtcaggc cgacgaagcc cagatacatg gtcggcgggt
     1621 gaatgaccat gtagaagttc tgcagcagcg gattcagccc ggtgccgtcc gtcgggcttt
     1681 ccgtcaggaa cgtcgcgaac ggattgttgt ggacgaccat cagcagcagg aaaaagaact
     1741 gcgtgacgga gatcgtcgcg acgacgtacg gaatcagctc gcggtgccgc tcgcgattga
     1801 cgtagacggc cgcgctgccg aagaacgaca gcagcgtgac ccagaacatg atcgagccgt
     1861 cgagcccgcc ccagtacgac gccagcttgt agaacatcgg cagcgccgaa tccgagtagc
     1921 gctggacgta cttgatcgag taatcgccgg tgacgaagga gtagatgatg acggccgagg
     1981 ccgccgccat gatggccgcg atcaggtgga aggcgccgat accgctctcg atcagcgccc
     2041 gtgaccggcg gcgcgcgccg gcaaccgaga tggcagccgc atacgccgca gtcacgaagg
     2101 ctgcgagcag caggtaatag cctaatgacg ccatttattt actcagggag ctgcccggcg
     2161 cttcctcgta cttggacgga cacttcgccg acatctgggt ggcgtggaac gtgccgttgt
     2221 ccgcaagccg cccggtcagc acgacctcgg attcgtcctt gaacgtatcc ggcacgatcc
     2281 cggtgtagta cgcgcgcagc accttgccgt tgcgctcgat gtcgaattga tattcgagcg
     2341 agtcgcgctt gcggccgatc gacttcggca ccacgtaccc gtgcacctgg aggttcttgt
     2401 cctgccaggc ctgcggctcg gcgagcacct catccacgaa cttgtagtac tgcatgctct
     2461 cgccgagcgt cgtgtacagc aggatcccga aggctgtggc gaggacaagg gtcgtaaacc
     2521 cgactttggc ggctttgtta ctcatagaaa aggagttgta agctcaggat agcccctcga
     2581 cttcggtcag ggcaagtccg ctttgcgcaa ttcttcagtg ccgggacgcg ccctttccca
     2641 gcgtccacat ccctaacccc acgacagagg cgaaaattcc ggccgccgcg accggatgca
     2701 gcatcgccct ggtgtagagg ctcgactctt tcgtccggcc cttccagtta ctgccgcgct
     2761 cgccaccgtc gcgggcgacc gctcgataca agttgtgggg cctgtcaggc gatgcaggtt
     2821 tgtcggtctt ctgcccctgg aacgtgaact tctccatgta gaggtcggcg gcccgggcgg
     2881 caaagcgggc ggcgctcagc atcaccgcgg ccccgccggc accgatctcc cggatgggcc
     2941 gccgcgcagc gtggatgatc acatcggcga cgacctccgg cgcgtacacc ggcggcaccg
     3001 gctgaggctc gacgccaagg taggtctttg ctttgtcgaa gaacggcgta tcgatgctgg
     3061 ccggcttcac cagcgtgacg gagatggggg ccccctcgtc ctcgagctcc atgcgcagcg
     3121 tgtcggtaaa cgccttcagc gcgtgcttcg cggcgcagta gttgccctgc agcgggatcg
     3181 cgcggtccga cag
//
LOCUS       DATLFO010000250         4783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87913, whole genome shotgun sequence.
ACCESSION   DATLFO010000250 DATLFO010000000
VERSION     DATLFO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87913"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VM841_10150"
     CDS             complement(<1..1267)
                     /locus_tag="VM841_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVL90580.1"
                     /translation="MTTSRRDFLKGAAAAGAMLLTRPLGALSSPLGPSPAFATERASR
                     LFGGRWLAHADLHNHTLLSDGSGNASLAFDSMRAAGLDVAALTDHSTLSWGMPQSVCN
                     GQKDCQGLAGINEGSWELTRVLADQANVDGSFVAIRGFEWSSPTLGHINVWGSERWMD
                     PLHTGGAGTGEGAAQFAHDEADLPAEQMAALDAIVKASPAQGAGMALFYDWLLAQPAH
                     PGLGGGADGLAGFNHPGREPGRFTNFRLDPRMRGRMSSLEVFNRNEDYLFEGTDSIAI
                     SPINECLNAGWRVGLLGVTDEHGTNWGYPDGKGRAGLWVTSLTRHGVAEAIRARRFFS
                     TRLRGLRLDASANGHPMGAEFAHTSGPITFRLDIDRGAAWTGTALKVSVLRSGATMPA
                     IQHVADVVVPGPADPVIEFTVDVDRADGDW"
     gene            1437..2495
                     /locus_tag="VM841_10155"
     CDS             1437..2495
                     /locus_tag="VM841_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90581.1"
                     /translation="MTEKDRIEDLQRALEGEGSSSEETASLLALVRMLEDLPEPEIDP
                     AFAASLEDRIVGGTAESPPAPAAGAPAGGSLAAITRIEERRSRRVRRGLVAAIAATFV
                     MALPVAASAEAVPGSPGYGVRLAREEARLWMTCRAGAVKCGFAHLDRASARLTDLRSV
                     IRRGDVSQVPATSARLRSSVSEGAATVLAAHPPATTLRRLAARLDETAARLGELAASA
                     PPSMRNPIREALGEGVSITHEIKVALGEAPPVRIVPVRPPVAIAPPVVAEPEPDAAPA
                     EDDRSRERTRRPSRKPADKGNSFGPGGTPKVGEPAPGEEWHADDPMRDGCPLGVVAAP
                     GVDPGVTAVCGAAGGGSR"
     gene            2492..3193
                     /locus_tag="VM841_10160"
     CDS             2492..3193
                     /locus_tag="VM841_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90582.1"
                     /translation="MRRLLLSVVVLAVALAGGAVRAAPACPSARTCDAFKLSSQRWAA
                     TKGVVTIPYQVNWLGPGSMPGDVTRAIIAAADAWEAANPRIRFKFEGTSYDVPQLGNN
                     RNEIAFVPTLEPGVIAQANHRAKGSVRLESDTLLNLAKPWSFDPCAQQNGSCADAEGP
                     ALRLLDLQSVVAQQLGNWLGLHTLSSDATRELTMNGTITYGERRKATLGLGDVKGVRA
                     AYPCGGCSSPRIFTP"
     gene            3262..3645
                     /locus_tag="VM841_10165"
     CDS             3262..3645
                     /locus_tag="VM841_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90583.1"
                     /translation="MRFRKAFAVAGFAAGLLAALPAHAAGAAFGTGAVGAGCNGSLGL
                     AAANSSGNNWVFDVSVSCTGSAPQHVIIAGQWDPATGGPVVGFGNGSLNVGKVVCGAN
                     QSVGVQVAMVGSPLRTGTASITRFC"
     gene            3719..>4783
                     /locus_tag="VM841_10170"
     CDS             3719..>4783
                     /locus_tag="VM841_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90584.1"
                     /translation="MIALRALVATAALLALAVPAGAVAAPAIPIPQSSSSAAPHYLGA
                     PASADPLTSFPVPRHPFMAANGRSNLHNDAYQTDAYDGGGPLGVSPAIRSTFHVAECA
                     SVTFDAAGRIVTICVGFDGPRLVLMNPHSLDTLAAFPLPPRNPLTLASVFSSFSGGGY
                     FYLDHLDRAVIPTTDGRILIVGVAETAAGPAFVPAAAYDLAPHVPPGDGIVSVLPDWD
                     GLLWFVTDSGVVGTVDPATSDVRSVTLAGEAIANSFAVDETGGVFIVSDHALYRFDAA
                     PGGTPSVTWREVYDRGTRRKPGQVSQGSGTTPTLVGSDFVAITDNADPKMNVVVYRRG
                     AQVAGGRFVCQAGVFADGVSN"
BASE COUNT          642 a         1748 c         1689 g          704 t
ORIGIN      
        1 tccagtcgcc gtcggcgcga tcgacgtcca cggtgaactc gatcaccgga tccgccgggc
       61 ccggcacgac gacgtcggcc acgtgctgga tcgccggcat cgtcgccccc gaccggagca
      121 ctgagacctt cagcgccgtg ccggtccatg cggcgccgcg gtcgatgtcg agccggaacg
      181 tgatcgggcc gctcgtgtgc gcgaactcgg cgcccatcgg atggccgttc gccgacgcgt
      241 ccaggcgcag tccgcggagc cgggtcgaga agaaccggcg cgcgcggatc gcctcggcga
      301 cgccgtggcg cgtaagcgag gtcacccaca gcccggcgcg gcccttgccg tcggggtagc
      361 cccagttggt gccgtgctcg tcggtcacgc cgagcagccc gacccgccag ccggcgttga
      421 ggcattcgtt gatcggcgag atcgcgatcg agtcggttcc ttcgaacagg tagtcctcgt
      481 tgcggttgaa cacctcgagg ctcgacatgc gcccgcgcat ccgcggatcc agacggaagt
      541 tcgtgaagcg tcccggctcg cgtccgggat ggttgaaccc ggcgagcccg tcggcgcccc
      601 cgccgaggcc gggatgcgcg ggctgggcga gaagccagtc gtagaacagc gccatgcccg
      661 caccctgcgc gggcgaggcc ttcacgatcg cgtcgagcgc cgccatctgc tcggcgggaa
      721 gatcggcctc gtcgtgcgcg aactgcgcgg ccccttcccc ggttccggcg ccgccggtgt
      781 gcaggggatc catccagcgc tcgctgcccc agacgttgat atggccgagc gtcgggctcg
      841 accattcgaa gccgcggatc gccacgaacg atccgtcgac gttcgcctgg tcggcgagca
      901 cgcgggtgag ctcccacgag ccttcgttga taccggcgag cccctggcag tccttctggc
      961 cgttgcacac gctctgcggc atgccccacg acagcgtcga gtggtccgtg agcgccgcga
     1021 cgtcgagtcc ggcggcgcgc atcgagtcga acgccagcga agcgttgccg gatccgtcgg
     1081 acagcagggt gtggttgtgc agatcggcgt gcgcgagcca gcgtccgccg aacagccggg
     1141 aggcgcgctc ggtcgcgaag gcgggcgagg ggccgagcgg gctcgacagg gcaccgagcg
     1201 gccgcgtcag cagcatcgcg ccggccgccg ccgcgccctt caggaagtct cgtctggagg
     1261 tggtcatagt gcgcggatac ttccccgggc gggtaccgaa atccttccga tgtccgaagt
     1321 cgtcatgatt tgttcccgcc cgcgacgagg acatatccgg catcgtcgcg cgcgcggcag
     1381 gaagggcccc cggccgaggt gaacctttca taccgaggca taccctaggg cccggcatga
     1441 ccgaaaaaga ccgcatcgaa gacctgcagc gcgcgctgga aggcgagggc tcctcctccg
     1501 aggagaccgc atccctgctc gcgctcgtgc gcatgctcga ggacctgccc gaacccgaga
     1561 tcgatcccgc gttcgccgcc tcgctcgagg accggatcgt gggcggcacg gccgaatcgc
     1621 cgcccgcgcc cgccgcgggc gcgcccgccg gcggctcgct cgccgcgatc acccgtatcg
     1681 aggagcgccg ctcccgccgc gtccgccgcg gcctcgtcgc cgcgatcgcg gccaccttcg
     1741 tgatggcgct gccggtggcg gcctccgccg aggcagtgcc gggctcaccc ggctacggcg
     1801 tgcggctcgc ccgcgaggaa gcccgcctgt ggatgacctg ccgggccggt gcggtcaagt
     1861 gcggcttcgc tcatctggat cgcgcgagcg cgcggctgac cgaccttcgg agcgtgattc
     1921 gtcgcggcga cgtctcgcag gtccccgcga cgtcggcgcg ccttcgctcg tcggtgtcgg
     1981 agggcgccgc gacggtgctc gcggcacatc cgccggccac gacgctgcgg cggctcgccg
     2041 cccgtctgga cgagaccgcc gcgcggctcg gcgagctcgc cgcctcggca cccccgtcga
     2101 tgcgaaaccc gatccgggaa gcgctcggcg agggcgtgtc gatcacccat gagatcaagg
     2161 tcgcgctcgg ggaagcgccg ccggtgcgca tcgtgccggt gcgtccgccc gtggcgatcg
     2221 cgccgccggt cgtcgccgag cccgagcccg acgccgctcc cgcggaagac gaccgctcgc
     2281 gtgagcgtac gcgccgcccg tcgcgcaagc cggcggacaa gggcaactcg ttcggaccgg
     2341 gcggcacgcc caaggtcggc gagccggccc cgggcgagga gtggcacgcc gacgacccga
     2401 tgcgcgacgg atgcccgctc ggcgtcgtgg ccgcgcccgg cgtcgatccc ggcgtgacgg
     2461 cggtctgcgg cgccgcggga ggtggctcgc gatgaggcgg ctgctgcttt cggtcgtggt
     2521 gctggcggtc gcgctggcgg gcggcgccgt gcgcgcggcg cccgcgtgcc cgtcggcccg
     2581 cacctgcgac gccttcaagc tttcgtccca gcggtgggcc gcgacgaagg gcgtggtgac
     2641 catcccgtat caggtcaact ggctcggccc cggatcgatg ccgggcgacg tgacccgcgc
     2701 gatcatcgcg gccgccgacg cgtgggaggc ggcgaacccg cgcatccggt tcaagttcga
     2761 gggcacctcc tacgacgtgc cgcagctggg aaacaaccgc aacgagatcg cattcgtgcc
     2821 gacgctcgag ccgggcgtga tcgcgcaagc gaaccatcgc gcgaaaggct cggtgcggct
     2881 ggagtccgac acgttgctca acctggccaa gccgtggtcg ttcgacccgt gcgcgcagca
     2941 gaacggatcg tgcgccgacg ccgagggccc ggccctgcgg ctgctcgatc tgcagtcggt
     3001 ggtcgcccag cagctcggca actggctcgg gctgcacacg ctgtcctcgg acgcgacccg
     3061 cgagctcacc atgaacggca cgatcaccta cggcgagcgg cgcaaggcga cgctcggcct
     3121 cggtgacgtc aagggcgtgc gcgccgccta tccgtgcggc ggatgctcga gcccccgaat
     3181 cttcacgcca tgaccgtttc ggccgcgaaa tcggaccttc ggggaaggag tcgcgccgcg
     3241 gctccgcgaa ctcttccgcc gatgagattc cgcaaagcgt tcgccgtcgc cggctttgcc
     3301 gccggcctgc tcgccgccct gcccgcccac gccgcgggcg cggcgttcgg caccggcgcg
     3361 gtcggggccg gatgcaacgg ctcgctgggg ctcgcggccg cgaactcgtc gggcaacaac
     3421 tgggtcttcg atgtctcggt gtcgtgcacg ggatcggcgc ctcagcatgt gatcatcgcg
     3481 ggacagtggg acccggcgac cggcgggccg gtcgtggggt tcggcaacgg atcgcttaac
     3541 gtcggcaagg tcgtctgcgg cgccaaccag tccgtcggtg tgcaggtcgc gatggtgggc
     3601 agcccgctga ggaccgggac cgcctcgatc acccgtttct gctagcccgg gaaggatccc
     3661 cggcgcgccg gatcgaacgt ttctttcgaa acgatctccg ctttcgaaag ggatgcccat
     3721 gatcgcgctc cgtgctctgg tcgcaaccgc cgccttgctc gcgctcgccg tgcccgccgg
     3781 cgcggtcgcg gctcccgcga ttccgatccc gcagtcgtcc tccagcgccg cgccgcacta
     3841 cctcggcgcg cccgcatcgg ccgatccgct cacctcgttt ccggtgccgc ggcatccgtt
     3901 catggccgcg aacggacgca gcaacctgca caacgacgcc taccagaccg acgcctacga
     3961 cggcggcgga ccgcttggcg tctcccccgc gatccgatcc accttccacg tcgccgagtg
     4021 cgcctcggtc accttcgacg ctgcggggcg catcgtgacg atctgcgtcg gcttcgacgg
     4081 gcctcgcttg gtgctgatga acccgcactc gctcgacacg ctcgcggcgt tcccgttgcc
     4141 gccgcggaac ccgctcacgc tcgccagcgt gttctcgtcg ttcagcggcg gcggctactt
     4201 ctacctggac cacctcgacc gcgcggtgat cccgacgacg gacggccgaa tcctgatcgt
     4261 gggcgtggcc gaaacggcgg ccggacccgc gttcgttccg gccgcggcct acgacctggc
     4321 gccgcacgtg ccgcccggtg acggcatcgt gtcggtgctg ccggactggg acgggctgct
     4381 gtggttcgtc accgactccg gcgtggtcgg caccgtcgat ccggcaacgt cggacgtgcg
     4441 ctcggtcacg ctcgcgggcg aggcgatcgc gaactcgttc gcggtggacg agaccggcgg
     4501 cgtcttcatc gtgtcggacc acgcgctcta ccgcttcgac gcagcgcccg gcggcacgcc
     4561 gtcggtcacc tggcgcgagg tctacgatcg cggcacgcgc cgcaaacccg ggcaggtctc
     4621 gcaaggatcc ggcaccacgc cgacgctggt gggatcggac ttcgtcgcca tcaccgacaa
     4681 tgccgacccg aagatgaacg tcgtcgtcta ccgccggggc gcgcaagtcg cgggcggccg
     4741 cttcgtgtgc caggcgggtg tgttcgccga cggtgtgtcg aac
//
LOCUS       DATLFO010000251         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_114968, whole genome shotgun sequence.
ACCESSION   DATLFO010000251 DATLFO010000000
VERSION     DATLFO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114968"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VM841_10175"
     CDS             complement(<1..863)
                     /locus_tag="VM841_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HVL90585.1"
                     /translation="MKSAGIVGLPDAGASQIFTALTAIEDGGATGKAHRARVPVPDDR
                     LPVLGKLHDSKKLVHAQLEFVDTSSMVRRGSRGAGTLPAELLGYLRESDALLQVVRGF
                     PLGDPADPAGELEALDLELTYADLEVVTGTIDRGGKAARLGDAEAKKQIAALEKAKEI
                     LDRGATLRSESWDDEERAVLRDLSLLTIKPALIVVNTGEGGDVAMPEGAVPVAGALEA
                     EVAGMSDEEATELLASFGQTERGLDTVIRGIYRQLDLITFLTAGDTESRAWEVRRGAP
                     APEAAGAIHSD"
     gene            complement(860..2629)
                     /locus_tag="VM841_10180"
     CDS             complement(860..2629)
                     /locus_tag="VM841_10180"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HVL90586.1"
                     /translation="MFSKVLVANRGEIAIRVFRALREMGIGSVAVYSEADRDAMHTAY
                     ADEAILLGPAAAAESYLNIDKILDAAERTGAEAIHPGYGFLAENENFAQAIEDAGLVW
                     VGPPPSAIVTMGDKVSARQAAVKAGAPVVPGTIDPITDPAEIRAFAEEHGLPIAIKAS
                     FGGGGRGFKVVRTMEEMEDALGSAQREAAMSFGRDEVYLERYLEGAKHIEAQIVADKH
                     GNVLFFGERECSMQRRHQKLVEEAPSPVLTPEVRERLREACVAICKQVGYVNAGTVET
                     LLDADRERFYFMEMNTRLQVEHPVTEMVTGVDLAKLQILVAAGEKLPLTQDQIQMRGH
                     AIEVRVNAEDPSKKFMPSPGRIGRYRVPTGPGVRVDSGFGENTEISRFYDSLVAKLIV
                     WAEDRDAARMRMVRAIDEYVIEGIKTTLPFHRIAMTDPGFASGEYSTSYVETEMDLSG
                     LKKSEPAAKVAAPDAPDARTLTVEVNGKRFEVRVDGLGAAGTTAAAATPAGGKPSAPV
                     AESAAVHGGGGAMDVVAPMQGTIVKTVVEAGATVKAGDLLIVLEAMKMENHITAPRDG
                     TVASLSVKPGQNVEGGAVVAVIE"
BASE COUNT          367 a          928 c          920 g          443 t
ORIGIN      
        1 atgtcgctgt ggatcgcgcc ggcggcctcg ggtgcgggag cgccgcggcg gacctcccac
       61 gcgcgggact cggtgtcgcc tgccgtcagg aacgtgatca ggtcgagctg gcggtagatc
      121 ccgcggatca cggtgtccag cccgcgttcc gtctgcccga acgaggcgag aagctcggtg
      181 gcttcctcgt cgctcatgcc ggcgacctcg gcctcgagcg cgccggcgac cggcacggca
      241 ccttccggca tcgcgacgtc gccgccttcg ccggtgttga ccacgatcag ggccggcttg
      301 atcgtcagca gcgataggtc gcgaagcacg gcccgctctt cgtcgtccca ggattcgctg
      361 cgaagcgtcg cgccgcgatc caggatctct ttggccttct ccagcgcggc gatctgcttc
      421 ttggcttcgg cgtcgccgag gcgcgcggcc ttgccgccgc ggtcgatcgt gccggtcacg
      481 acctcgaggt cggcgtaggt cagctcgaga tcgagcgctt ccagctcgcc ggcaggatcg
      541 gccggatcgc cgagcggaaa gccccgaacg acctgcagca gcgcgtcgga ctcgcgaagg
      601 tagccgagca actcggcggg aagcgtgccc gcgccgcgcg agccgcggcg caccatgctg
      661 gaggtgtcca cgaactcgag ctgcgcgtgc acgagcttct tggagtcgtg cagcttgccg
      721 agcaccggca agcggtcgtc gggaacgggc acgcgcgcgc ggtgcgcctt gccggtcgcg
      781 ccgccatcct cgatcgcggt gagcgcggtg aagatctgcg aggcgcccgc gtcgggcagg
      841 ccgacgatgc cggcggactt cattcgatca cggccacgac cgcgccgcct tcgacgttct
      901 ggccgggctt gacgctgagc gaggcgaccg tgccgtcgcg cggcgcggtg atgtggttct
      961 ccatcttcat cgcctcgagc acgatcaaaa gatcgccggc cttcacggtc gcgccggctt
     1021 cgacgacggt cttgacgatc gttccttgca tcggcgcgac gacatccatg gcgccgccgc
     1081 ccccgtgcac ggccgcggac tcggcgaccg gcgcggaggg cttgccgccg gcgggcgtcg
     1141 cggccgctgc ggtcgtgccg gccgcgccca gaccgtccac gcgcacctcg aagcgtttgc
     1201 cgttcacctc aacggtcaag gtgcgcgcgt cgggcgcgtc gggcgcggcg accttggcgg
     1261 cgggctcgga cttcttcagg ccgctcagat ccatttcggt ctcgacgtag ctggtcgagt
     1321 actcgcccga ggcgaagccg ggatcggtca tcgcgatccg gtggaacggg agcgtggtct
     1381 tgatgccttc gatcacgtac tcgtcgatcg cgcggaccat gcgcatgcgt gccgcgtcgc
     1441 gatcctcggc ccacacgatc agcttcgcga ccagtgagtc gtagaagcgc gaaatctcgg
     1501 tgttctcgcc gaagcccgag tcgacgcgca cgccgggacc ggtcggcacg cggtagcggc
     1561 cgatgcggcc cggcgaaggc atgaacttct tcgagggatc ctcggcgttg acgcgcacct
     1621 cgatcgcgtg gccgcgcatc tggatctgat cctgcgtcag cggcagcttc tcgccggccg
     1681 cgaccaggat ctgcagcttc gccagatcca cgccggtgac catttcggtg acgggatgct
     1741 cgacctgcag gcgcgtgttc atctccatga agtagaagcg ttcgcggtcg gcgtccagca
     1801 gcgtctcgac ggtgccggcg ttgacgtagc cgacctgctt gcagatcgcg acgcaggctt
     1861 cgcggagccg ctcgcgcacc tcgggcgtca gcaccggcga cggcgcttcc tcgaccagct
     1921 tctggtggcg gcgctgcatg ctgcattcgc gctcgccaaa gaacaggacg ttgccgtgct
     1981 tgtcggccac gatctgcgcc tcgatgtgct tggcgccctc caggtagcgc tccaggtaga
     2041 cctcgtcgcg gccgaagctc atggcggcct cgcgttgcgc gctgccgagc gcgtcttcca
     2101 tctcttccat cgtgcgcacg accttgaagc cgcgaccccc gccgccgaat gaggccttga
     2161 tcgcgatcgg cagaccgtgc tcttcggcga aggcgcggat ctcggccggg tcggtgatcg
     2221 ggtcgatcgt gccgggcacg acgggcgcgc cggccttgac cgccgcctgc cgtgccgaga
     2281 ccttgtcgcc catcgtcacg atcgccgacg gcggcgggcc gacccacacc agccccgcgt
     2341 cctcgatcgc ctgggcgaag ttttcgttct cggccaggaa gccgtagccg ggatggatcg
     2401 cctcggcgcc ggtgcgctcg gcggcatcca agatcttgtc gatgttcagg tacgactcgg
     2461 ccgccgcagc cgggccgagc aggatggcct cgtccgcgta cgcggtgtgc atcgcatcgc
     2521 ggtcggcctc ggaatagacg gcgacgctgc cgattcccat ctcgcgcagg gcgcggaaaa
     2581 cgcggatcgc gatctcgcca cggttggcga ccaggacctt ggagaacatg acgcgcgaag
     2641 gctaccagag cgcccggc
//
LOCUS       DATLFO010000252         8184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_87956, whole genome shotgun sequence.
ACCESSION   DATLFO010000252 DATLFO010000000
VERSION     DATLFO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87956"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VM841_10185"
     CDS             <1..1507
                     /locus_tag="VM841_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVL90587.1"
                     /translation="FLYSSFLLGGGPDSGWFAYSPVTGPKFSPGHRMDFYALGLIITG
                     ISSTVSAVNLLVTIVNMRAPGMSLMRMPIFTWMSLVVQFLLVFALPIITVDLFYLFFD
                     RNLGTLFFDSAAGADPLLWQHLFWLFGHPEVYILILPAMGIVSEILPVFARKPLFGYP
                     FVVFAGAAIGFMGWGVWAHHMFATGMGPVANSAFAISTMFIAVPTGVKIFNWLATIWK
                     GQIRFTLPMLFSLAFVMQFVLGGLSGVSHAFVPHDYQQTDTYYIVAHFHYVLMGGAIF
                     GLFAGIYYWWPKITGRFLNERAGKVHFWLMTIGFNLTFAPFHILGLQGMPRRIYTYSD
                     GYGWNAWNMVATAGSLLIAVSVLAFIWTVWVSRKGKGAKASGDPWDARTLEWMTPSPP
                     PEYNFVEIPEVHALDDFWHRKYVENPEGRPMPVPAGGQAAASEHPQGTAEELGIHMPS
                     PSYMPILASLGFPMVGYAVIYKPWWAIPGAIVLIIGLFGWAMEPATEDSHG"
     gene            1500..2111
                     /locus_tag="VM841_10190"
     CDS             1500..2111
                     /locus_tag="VM841_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HVL90588.1"
                     /translation="MAEATTTAPAHEEHHTSTGLPHVKLAMWGFLASECLLFGALIST
                     YLLYRGKAESGPLPHEVYDIPFTSVSSFVLLMSSLTMVLALAAIQRGDHHRTRVWLVC
                     TGLLGAVFLAGQVYEFTVFVNEGFTVRSSVAGSAFFVLTSFHGAHVTLGIVMLLFLYG
                     LSVRGKLPTERADAVEYVGLYWHFVDVVWIVIFTLVYLIPPPN"
     gene            2124..2435
                     /locus_tag="VM841_10195"
     CDS             2124..2435
                     /locus_tag="VM841_10195"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HVL90589.1"
                     /translation="MSTTNVHKAATDHDHPGPWQYVQIATFLAVITAAEVALYYIKMP
                     DALLVFLLFVFSAIKFYLVVRYFMHLKFDAPIYRKLFLIGIILAFTVYTVVLLTFGLL
                     R"
     gene            2452..3312
                     /locus_tag="VM841_10200"
     CDS             2452..3312
                     /locus_tag="VM841_10200"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVL90590.1"
                     /translation="MVAASSPWGFHMHPDVWLLVVALTGGYWWMCRRERERNAGNALA
                     EPPATRTQKWLFGSGVFVLWLGADWPIHDVAEGSMYSVHMIQHMLFTLGAAPLLLLGT
                     PAWMFRRLVRPAPMFKAVRTMSRPFVALVFFNVVLIFTHWPAMVTASVGSELVHLSLH
                     VLIMASALAMWMPVASPVMEIPRMSYPGQMVYLFLQSLVPTIPASFLTFGSTPLYSVY
                     VDMPKLWGISPMTDQLTAGLIMKIVGGFILWGVIATLFFKWYKIEQSEGIDVLALSRL
                     DRELNRGATR"
     gene            3309..4298
                     /locus_tag="VM841_10205"
     CDS             3309..4298
                     /locus_tag="VM841_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVL90591.1"
                     /translation="MSDFRKRILEPLLLPLAAFAFVGALAFGLSRILLVTTANGASLV
                     ALVAALAVLLSASAVAAHGFGKPEKLMALFSFVAIVAGGSVFAATIGMRPVEHHTPEP
                     SFVFTAVNSVSFEQSEATVETADELVIRFTNKDPAAPHNWGVQAEPTFAASPSLVEPG
                     PGLDNGQSRDYILEHPDPGTYYYFCFIHPNMKGTLTITGEGGEAPPPPTPAESPTGSP
                     SPTAGTPQPTETAPAGPAATSVDITAIDIAFDKDSLTLKAGTEIAVTFRNNDAGVPHN
                     FAIYSVDLSRTIFDTKIFNGVETRTGTFTSPAPGTYVFRCDVHPQMKGDVVFE"
     gene            4295..4903
                     /locus_tag="VM841_10210"
     CDS             4295..4903
                     /locus_tag="VM841_10210"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HVL90592.1"
                     /translation="MRPQHPPARPVDRRAFLRAAGLAGLAIPLAACGDGGSKTAEPTK
                     GGSPAPSAPTSGTEAAGASGLSIGDEIPAIRSEAVEGPEIKLEQYRGHPFVLNFWAST
                     CEPCRREFPLLRGLIERHSDLPIVGVDVMERKEPAAEFARSQDATWPSIWDPDGSISA
                     KFRVRVLPVTYAIGRNFKLVDRHFGELTRERLDQMVDAMLKS"
     gene            complement(4900..6846)
                     /locus_tag="VM841_10215"
     CDS             complement(4900..6846)
                     /locus_tag="VM841_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL90593.1"
                     /translation="MTESLVEGRLAAGSGRWGEAFELLGTLDPSLLSAEDHEALAEAA
                     FWRGHMRRSIEARESAYRLYLSEGRMAAAAKCAIKLATAYGDLMERPFVGVWLKNAEN
                     ALAGEPEGPEHARLELERSFQSTVGNVAGDPVAALERGLEIAGRVGDKNVLALGLAMK
                     GMMTLHSGDVETGMRLLEEATIPAVSGELEPYVTGIIYCMVISGSSQIADWQTAGQWT
                     EAAKRWCERQSINGFPGVCRVHRAEVMRLRGSFALAEEEARTATEELLHFNIGAAGVA
                     FRELGDVRMRMGELDAAEAAFRQAHEIGVIPQPGLALLQAMRGKTANAIASLRRAIDD
                     EAVGPADRAKIFPVLAELALKSGDAATAESCLASLEAIASTYRSDALEAAVLTTRARL
                     LRSRGELAEAESAAKRALRLWRSVDLLYDAAHTQMLLGDIYLDQDDKDAADFEYTSAL
                     SMLERFGAVLDAETVRQRRTACLDEPARSDESVRMAFLFSDIVGSTSLVDAIGDDAWI
                     DLLKWHDEQLLSCFGTHHGTVVAHTGDGFFVAFGSARDAIGCAVDVQRRLAAHRRSHG
                     FAPQVRIGVHVTEARRSHGNYHGKGIHEAARIGALAGGGEVLVSVATVEEAGIPVKAG
                     DVREETLKGVAEPARVVSLDWRTI"
     gene            complement(7140..>8184)
                     /gene="groEL"
                     /locus_tag="VM841_10220"
     CDS             complement(7140..>8184)
                     /gene="groEL"
                     /locus_tag="VM841_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HVL90594.1"
                     /translation="FDKGYISPYFVTDQERMEAVLDDPYILLVSKKISSVQEMLPVLE
                     KVMQSGKALLIIAEDVEGEALATLVVNKIRGTFRSLAVKAPGFGDRRKAMLQDIAILT
                     GGQVISEEVGLKLENTTLDMLGRARKMVVTKDETTLVEGAGDAEQIKGRINQIKAEID
                     KTDSDYDREKLQERLAKLSGGVAVIKVGAATEVELKEKKHRIEDAVSATKAAVEEGIV
                     AGGGVTLLQAQSALEKLELEGDEKTGASIVFKALEYPVKQIAENAGFEGGVVVEKVRG
                     LDIGWGLNADNGEYVDMFKVGVIDPAKVTRSALQNAASIAALFLTTEAVVAEKPEKNP
                     APAAPGMGGHDEF"
BASE COUNT         1230 a         2847 c         2617 g         1490 t
ORIGIN      
        1 gttcctgtac tcgtcgttcc tgctgggcgg cgggcccgac tcgggctggt tcgcttactc
       61 gccggtgacc gggccgaagt tctcgcccgg ccatcggatg gacttctacg cgctcggtct
      121 gatcatcacc ggcatctcgt cgacggtctc ggcggtcaac ctgctggtca ccatcgtcaa
      181 catgcgcgcc cccggcatga gcctgatgcg catgccgatc ttcacctgga tgtcgctggt
      241 cgtgcagttc ctgctggtgt tcgcgctgcc gatcatcacc gtggacctgt tctacctctt
      301 cttcgatcga aatctcggga ccctgttctt cgactcggcc gccggagccg acccattact
      361 ttggcaacac ctgttctggc ttttcggcca tcccgaggtc tacatcctga tcctgcccgc
      421 catgggcatc gtgtccgaga tcctgccggt cttcgcccgc aagccgctgt tcggttaccc
      481 gttcgtggtc ttcgccggcg cggcgatcgg cttcatgggc tggggcgtgt gggcccacca
      541 catgttcgcc accggtatgg gcccggtcgc caactccgcg ttcgcgatct cgacgatgtt
      601 catcgcggtt ccgacgggcg tgaagatctt caactggctc gcaacgatct ggaagggcca
      661 gatccggttc acgctgccga tgctgttctc gctcgcgttc gtgatgcagt tcgtcctcgg
      721 cggcctttcg ggcgtgtcgc acgcgttcgt gccgcacgat taccagcaga ccgacaccta
      781 ctacatcgtg gcccacttcc actacgtgct gatgggcggc gcgatcttcg gcctgttcgc
      841 gggcatctat tactggtggc ccaagatcac cggacgcttc ctgaacgagc gggccggcaa
      901 agtgcacttc tggctgatga cgatcggatt caacctgacg ttcgcgccgt tccacatcct
      961 cgggcttcag ggcatgccgc ggcgcatcta cacgtactcg gacggctacg gctggaacgc
     1021 ctggaacatg gtcgccacgg cgggctcgct gctgatcgcc gtctcggtgc ttgcgttcat
     1081 ctggaccgtg tgggtgtcgc gaaagggcaa gggcgcgaag gcgagcggcg acccgtggga
     1141 cgcccgcacg ctcgaatgga tgacgccctc accgcccccc gagtacaact tcgtggagat
     1201 ccccgaggtg catgcgctcg acgacttctg gcaccgcaag tacgtcgaga accccgaggg
     1261 acggccgatg ccggtcccgg ccggcggcca ggccgccgca agcgagcacc cgcagggcac
     1321 ggccgaagag ctcggcatcc acatgccgtc gccctcttac atgccgatcc tggcctcgct
     1381 cgggttcccg atggtcggct acgcggtgat ctacaagccg tggtgggcga tcccgggcgc
     1441 catcgtgctc atcatcggat tgttcggctg ggccatggag cccgcgacgg aggactcgca
     1501 tggctgaggc aaccacgacc gccccggcgc acgaggagca ccacacctcg accggactgc
     1561 cgcacgtcaa gctcgcgatg tggggcttcc tggcttcgga gtgcctgctg ttcggcgccc
     1621 tgatctcgac ctacctgctc tatcgcggca aagccgagtc cggtccgctg ccgcacgagg
     1681 tctacgacat ccccttcacg tcggtgtcct cattcgtgct gctgatgtcg tcgctgacga
     1741 tggtgctcgc cctggcggcg atccagcggg gcgaccacca ccgcacccgc gtctggctgg
     1801 tgtgcaccgg gcttctgggc gcggtgttcc tcgcggggca ggtgtacgag ttcacggtgt
     1861 tcgtcaacga aggcttcacg gtccgctcca gcgtcgccgg atccgccttc ttcgtcctga
     1921 cctccttcca cggagcgcac gtcacgctcg gcatcgtcat gctgctgttt ctgtacggct
     1981 tgtcggtacg cggcaagctt ccgacggagc gggccgatgc ggtcgagtac gtcggcctat
     2041 actggcactt cgtggatgtg gtttggatcg tgatcttcac gctcgtgtat ctgatcccgc
     2101 caccgaacta aaggcgaaag acaatgtcga ccacgaacgt ccacaaggca gcgacggacc
     2161 acgatcatcc gggtccctgg cagtacgtcc agatcgcgac cttcctggcg gtcatcacgg
     2221 ccgcggaggt cgcgctctac tacatcaaga tgccggatgc ccttctggtc ttcttgctgt
     2281 tcgtgttctc ggcgatcaag ttctacctgg tggtgcgcta cttcatgcac ctgaagttcg
     2341 acgcgcccat ctatcgcaag ttgttcctga tcggcatcat cctggcgttc accgtctaca
     2401 cggtcgtgct gctgaccttc ggattgctgc ggtagggaga cgcgcgtgag catggtggcg
     2461 gcgtcctccc cttggggctt ccacatgcat cccgacgtgt ggctgctggt cgttgctctc
     2521 accggcggct actggtggat gtgccgccgc gagcgcgagc gcaatgcggg gaacgcgctg
     2581 gccgagccgc ccgcgacccg cacgcagaag tggctcttcg gctcgggcgt gttcgtgctg
     2641 tggctgggcg cggattggcc catccacgac gtcgccgagg gctcgatgta ctcggtgcac
     2701 atgattcagc acatgctctt caccctcgga gccgcgccgc tgctgctgct gggaaccccc
     2761 gcctggatgt tccgccgcct ggttcggccc gctccgatgt tcaaggccgt gcgcacgatg
     2821 tcgcggccgt tcgtggcgct cgtgttcttc aacgtggttc tgatcttcac ccactggccg
     2881 gcgatggtga ccgcgagcgt gggcagcgag ctggtccacc tgtcgttgca cgtgctgatc
     2941 atggcatcgg cgctggcgat gtggatgccg gtcgcctcgc cggtgatgga gatcccgagg
     3001 atgagctatc cgggacagat ggtctacctg ttcctgcagt ccttggtgcc gaccatcccg
     3061 gcctcgttcc tgacgttcgg ctccacgccg ctctacagcg tctacgtgga catgcccaag
     3121 ctctggggca tctcgccgat gaccgaccag ctcacggccg ggctgatcat gaagatcgtc
     3181 ggaggcttca ttctgtgggg ggtcatcgcg actcttttct tcaagtggta caagatcgag
     3241 caatcggagg ggatcgacgt gctggcgttg agcaggctgg accgtgagct gaaccgggga
     3301 gcgactcgat gagcgacttc cgcaagcgta tcctcgagcc gctgctgctt cccctcgcgg
     3361 cgttcgcatt cgtcggcgcc ctcgcgttcg ggctctcccg gattctgctg gtcaccaccg
     3421 ccaacggcgc gtcgctcgtg gcgctcgtcg cggccctggc cgtgctgctg tccgcgtcgg
     3481 ccgtcgccgc ccacgggttc ggcaagcccg agaagctgat ggccctgttc tcgttcgtgg
     3541 cgatcgtcgc cggcggcagc gtgttcgccg ccacgatcgg catgcgcccg gtcgagcatc
     3601 acacgcccga gccgtccttc gtgttcaccg cggtcaactc ggtctcgttc gagcagtccg
     3661 aagcgacggt ggagaccgcc gacgagctgg tgatccgttt caccaacaaa gatccggcag
     3721 cgccgcacaa ctggggcgtc caggccgagc ccacgttcgc ggcgtcaccg agcctggtcg
     3781 agccgggtcc tggcctggac aacggccaga gccgcgacta catcctcgag catccggatc
     3841 cgggcaccta ctactacttc tgcttcatcc acccgaacat gaagggcacg ttgacgatca
     3901 ccggcgaagg cggagaggcg ccgccgccgc cgacccctgc cgagtccccg accggcagcc
     3961 cgtcgcccac cgcaggcacg ccacagccga ccgaaaccgc gcccgccggc ccggccgcca
     4021 cgtcggtgga catcaccgcg atcgacatcg cgttcgacaa ggactcgctg accctcaagg
     4081 ccgggaccga gatcgcggtc accttccgca acaacgatgc gggcgtgccg cacaacttcg
     4141 cgatctacag cgtggacctg tcccgaacca tcttcgacac caagatcttc aacggggtcg
     4201 agacccggac cggcacgttc acctcgccgg cacccggcac gtacgtgttc cgctgcgacg
     4261 ttcatccgca gatgaagggc gacgtggtct tcgagtgagg ccgcagcatc cgccggcccg
     4321 gccggtcgac cgtcgcgcgt tcctgcgcgc tgcgggactg gccgggctcg cgatcccgct
     4381 cgccgcgtgc ggcgacggcg ggtcgaagac cgcagaaccc accaaaggcg gctcgcccgc
     4441 tccctcggcg ccgacgtcgg gcaccgaggc ggccggggcg tccgggctga gcatcggcga
     4501 cgagatcccg gcgatccgct ccgaagccgt cgaaggaccc gagatcaagc tcgagcagta
     4561 tcgcgggcat ccgttcgtcc tcaacttttg ggccagcacc tgcgagccct gccggcgaga
     4621 gttcccgctg ctgcggggcc tgatcgagcg gcactcggat cttccgatcg tgggcgtgga
     4681 tgtgatggag cgcaaggagc ccgccgcgga gttcgcgcgc tcgcaggacg ccacctggcc
     4741 cagcatctgg gatccggacg gctcgatctc ggccaagttc cgcgtgcgcg tgctgcccgt
     4801 cacctacgcg atcggccgca acttcaagct ggtggaccgg cacttcggcg agctcacccg
     4861 cgagcgcctc gaccagatgg tcgacgcgat gctgaagtct tagatcgtcc gccagtcgag
     4921 cgagaccacc cgcgcgggtt ccgcaacgcc cttcaacgtc tcttcgcgga cgtcgccggc
     4981 cttgaccggg attccggctt cctcgaccgt ggcgacgctg acgagcacct cgcccccgcc
     5041 ggccagcgcg ccgatgcgcg cggcctcgtg gatccccttg ccgtgatagt tgccgtgcga
     5101 acggcgtgcc tcggtcacgt gcacgccaat ccgaacctgc ggagcgaacc cgtgcgatcg
     5161 gcgatgcgcc gcgagccgtc gctggacgtc caccgcgcat ccgatggcat cgcgagcgga
     5221 gccgaacgcc acgaagaacc cgtctccggt gtgggccacc accgtgccgt ggtgcgtgcc
     5281 gaagcacgac agcagctgct cgtcgtgcca cttgagcaga tcgatccagg catcgtcccc
     5341 gatcgcgtcc acgaggctgg tcgagcccac gatgtcggag aacaggaacg ccatccgcac
     5401 gctctcgtcc gagcgggcgg gctcgtcaag gcacgcggtg cgccgctgcc ggaccgtctc
     5461 ggcatccagg accgccccga accgctcgag catcgacagg gcgctggtgt attcgaagtc
     5521 ggcggcgtcc ttgtcgtcct gatccaggta gatgtctccc agaagcatct gcgtgtgcgc
     5581 ggcgtcgtag agcagatcga cggagcgcca cagccgcagc gcgcgcttgg ccgcgctctc
     5641 ggcctcggcg agctcgccgc gggagcgcag cagacgcgca cgagtcgtca ggaccgccgc
     5701 ctccagcgcg tcggaccggt acgtcgaggc gatcgcttcg agcgaggcca ggcaactctc
     5761 ggcggttgcg gcatcgcccg acttcagcgc gagctcggcc aggaccggaa agatcttggc
     5821 ccggtcggcc ggcccgaccg cctcgtcgtc gatcgctcgc cggagcgagg cgatcgcgtt
     5881 ggcggtcttg ccccgcatcg cctgcaggag cgccagcccc ggctgcggga tcacgccgat
     5941 ctcgtgcgcc tgccggaacg ccgcctcggc ggcgtcgagc tcgcccatgc gcatgcgcac
     6001 gtcgccgagc tcacggaacg cgacgccggc ggcgccgatg ttgaagtgca gcagctcttc
     6061 ggtggccgtg cgggcttcct cctcggcgag cgcgaaggag ccgcgaagcc gcatcacctc
     6121 ggcccgatgg acgcggcaga cgccggggaa gccgttgatc gattgccgtt cgcaccagcg
     6181 cttggcggct tcggtccatt gacccgcggt ctgccagtcg gcgatctggg aggaccccga
     6241 gatcaccatg cagtagatga tcccggtcac gtagggctcg agctcgcccg agaccgcagg
     6301 aatggtggcc tcttcgagca gccgcatgcc ggtttcgacg tcgccggaat gcaacgtcat
     6361 catgcctttc atcgccaggc cgagcgccaa gacgttcttg tcgcccacgc gcccggcgat
     6421 ctccaagcct cgttccaacg ccgcgaccgg gtcaccggcg acgttgccca cggtggactg
     6481 gaacgaccgc tcgagctcga ggcgggcgtg ctcgggcccc tcgggctccc ccgcgagcgc
     6541 gttctcggcg ttcttgagcc acacgccgac gaacggccgt tccatcagat ccccgtaggc
     6601 cgtcgcaagc ttgatcgcgc acttggcggc cgccgccatg cgcccctcgg acagatagag
     6661 ccggtacgcg ctctcgcgcg cttcgatgct gcggcgcatg tgtccgcgcc agaacgcggc
     6721 ctcggccagc gcctcgtgat cttcggccga taacagcgac gggtcgagcg ttccgagcag
     6781 ctcgaacgcc tcgccccacc ggccgctccc cgcagcaagc cgtccttcga cgagggattc
     6841 ggtcatggtc caattctcgc acacacgcgc cgccgagaaa cggggcccac ccgaattcgg
     6901 gactccgccg tatgcagggt ccggagaaaa gggaagccgg cgcctttgtg gggcaccggc
     6961 aaggggagga agatcgggtg gagactcgag cgacgaaaga ggctgacgac gggcctttta
     7021 cctttcggtc gaagtcccca cccgatccga taacgacccg gatgcccaga ggtgacgcgg
     7081 tttcggagaa aaattggagg cggcccgaag gccgcctcct gtgaaactgc gacgggttct
     7141 tagaactcgt cgtggccgcc catgccgggc gcggccgggg ccgggttctt ctccggcttc
     7201 tccgcgacga ccgcctcggt cgtgaggaac agggccgcaa tcgaggccgc gttctgcagc
     7261 gccgaacggg tcaccttcgc cgggtcgatc acaccgacct tgaacatgtc cacgtactcg
     7321 ccgttgtcgg cgttgaggcc ccagccgatg tccaggccgc gaaccttctc gaccacgacg
     7381 ccgccttcga agccggcgtt ctcggcgatc tgcttcacgg ggtactcgag cgccttgaac
     7441 acgatcgagg cacccgtctt ctcgtcgcct tccagctcga gcttctcgag agcggactgc
     7501 gcctgcagca gcgtgacgcc acccccggcg acgatgccct cttccaccgc ggccttggtc
     7561 gccgagacgg cgtcctcgat gcggtgcttc ttctccttga gctcgacctc ggtggcggca
     7621 ccgaccttga tgaccgcaac gccgcccgac agcttcgcca gacgctcctg cagcttctca
     7681 cggtcgtagt ccgagtcggt cttgtcgatc tcggccttga tctggttgat ccggcccttg
     7741 atctgctcgg cgtcgccggc gccctcgacc agcgtcgtct cgtccttggt gacgaccatc
     7801 ttgcgggcgc ggcccagcat gtccagcgtc gtgttctcga gcttgaggcc gacctcttcg
     7861 ctgatgacct ggccgccggt caggatcgcg atgtcctgca gcatggcctt gcgacggtcg
     7921 ccgaagcccg gggccttgac ggccagcgag cggaacgtgc cgcggatctt gttgacgacg
     7981 agcgtggcga gcgcctcacc ctcgacatcc tcggcgatga tcaggagcgc cttgccggac
     8041 tgcatgacct tctcgaggac cggcagcatc tcctgcacgc tcgagatctt cttcgagacg
     8101 agcaggatgt acgggtcgtc gaggacggcc tccatgcgct cctggtcggt gacgaagtac
     8161 ggcgagatgt agcccttgtc gaac
//
LOCUS       DATLFO010000253        10083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_36360, whole genome shotgun sequence.
ACCESSION   DATLFO010000253 DATLFO010000000
VERSION     DATLFO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36360"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1278
                     /locus_tag="VM841_10225"
     CDS             148..1278
                     /locus_tag="VM841_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90595.1"
                     /translation="MRIRGRLGIVGLLLAATIAAGPAPARAASPATLLDAIRSEADYI
                     LSLRTGDGVITTAPNRVVPYLANFAAMGLARAHLVLRDGRYATAAWRWLEWYRDHMHP
                     DGTMPDWVYSSSWVPTGDPDSTDAYAGTYLSAVLATFEATGDLLRLRALHGGVLKAVA
                     AIELTADVDGLHFAKPGWPFKYSMDEAEAYAGFRAAQRIGRALNDASLEARAKTNADR
                     LLSASIKLIDPATGLYLWALHQDGTRVVAPIEWIYPGASAQAWAVADGLATGSNARNL
                     MARVEAAQPFWDRPTATAQYANAQPNCNFATPCYAPVNYWPRFALAYLAIGNARRAMQ
                     GALSIHAAAKSLGRGFPYTPGDAGQIILTLADPQALAESLIP"
     gene            complement(1439..1972)
                     /locus_tag="VM841_10230"
     CDS             complement(1439..1972)
                     /locus_tag="VM841_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90596.1"
                     /translation="MAASLFVLGGLVPTAGHAASTPVECSGTVAANGTAECSVSFTIS
                     DFRYISNIGTYGNMIDLAATGTINMAWIDAKGQTVASWTCNGFGILGADVLTASGPSS
                     SLVGGTPASPCVQNGTTADNFAWRDAQGNLLPQTFRVSVASTTARAATFHGAMNLSQG
                     NCDATACYPDLIPIPPR"
     gene            2281..2643
                     /locus_tag="VM841_10235"
     CDS             2281..2643
                     /locus_tag="VM841_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HVL90597.1"
                     /translation="MFSFAHPGDVVRALERSRDCFDPSSTSLMIVAETKNPHRDPFGR
                     GFIAGFEERTELRKRLARLDERSRALVILWHAYGHPVAQIARRLGISRVHCYRLERRA
                     LDLMIDQPEGGCAEAVGA"
     gene            2828..3508
                     /locus_tag="VM841_10240"
     CDS             2828..3508
                     /locus_tag="VM841_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90598.1"
                     /translation="MTTVGYHLDNSFGIHVRGLRRNFGASSVLDGLTMDAPSGALTAV
                     SGANGAGKTTLLRILATLLRADGGDAWVAGAHVGREPERVRGRIGVALVNDRGLYWRL
                     SGSRNLEFFGRALGLSRADARTDAARVAAEMGIEGFAGRPVATYSAGQRQRLVLARAS
                     LADPAVLLIDEPMRGLDEDGIARARAMMLRHARRGAAVLVAAPTIAEFSDLCDTRIVL
                     RGGRAEAA"
     gene            3505..4356
                     /locus_tag="VM841_10245"
     CDS             3505..4356
                     /locus_tag="VM841_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL90599.1"
                     /translation="MSETLATAPAIAERRSPTVASAAGLFMGRELSVALSYKTAFVMN
                     AASVALSLVMFHFIAKLVTATAAGATLPGGYFSFVVVGLATAQILDGAVTQPSSLVRQ
                     EQVQGTLEVLATTPLRPAALAAGWISYPIAASLVNSLIMLALAGPLGFRFEDPNWPVG
                     VLALVLSSLAFAGFGILVAAVVLVIQQGAQLTRWISAGMGLLSGVFFPIELFPRWLQL
                     IAQASPLSHAVEALRGSMLLRRSAADLAPDLIALGVMAALLIPLSLLALSAALARARA
                     RGTISSY"
     gene            4389..4673
                     /locus_tag="VM841_10250"
     CDS             4389..4673
                     /locus_tag="VM841_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis PqqD family
                     chaperone"
                     /protein_id="HVL90600.1"
                     /translation="MPDEASDVEVPKEVLSRELDGEAVLLDLRSGRYFGLNGTGAVVW
                     SMLKDGAEREQIAHQITEEFAVDLVRARADVDAFIESLVGRGLITYRDRA"
     gene            4642..5133
                     /locus_tag="VM841_10255"
     CDS             4642..5133
                     /locus_tag="VM841_10255"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HVL90601.1"
                     /translation="MASSPTGTAPKRGTAARLARAARDPAETARRVAVLGKIVAARAM
                     IATGGFRLVRGVHRYRTVEQAERPLGPEPALTPAQAREIQRAIGLLDRLEKRVSCLPR
                     SVVLERVCRKAGVPAEIVIGVDRSKGFAAHAWVEIEGYPLGATEAGRAHWNALGRFRR
                     DGR"
     gene            5130..6959
                     /locus_tag="VM841_10260"
     CDS             5130..6959
                     /locus_tag="VM841_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HVL90602.1"
                     /translation="MSGFYAATGAAAALPDGLAGRLAGRAAGAMRSWSDEQVTLLAFE
                     GRSGPGLAVADDLVVALDGRIDEPRGTAPAADRLLNEWRRDGAKALDRLAGDFAGAVY
                     DRRATTLTVLRDPCAGRPAILGQVPGGLVAASTVPMLLEDPRLDREIDPEWVAAYLTA
                     SAGFATATGYRAMTRLIPGAAAEARGRDWTIVRRHTWRPAAVRERTDQAYADGLRERL
                     VRAIEDRTAGASRIGISLSGGLDSTSIAALLRRARPDAQIVALAVPFTHPRGDERALQ
                     KMVAERIGAELRWAPLDGTSPYGASAAATLDRLGQPPIAPNHFFVERVAAEGRAAGVE
                     VAMDGIDGDAVVGGNWFYLADLLVTGRLGAFRRELAAAARIHGVARKRIVKDYVAATL
                     LPDRLRALPAVPDYWDPAFVRKHRLAERLRGRSRGRPGRAFRANELAAMAPDVAPAIL
                     EQIGLAWGPVEVSHPFLDRRVVEYCLGLPRKQKVSAGVTKIVLRNALRGLLPPEVAER
                     SEKAELGKAFFEGLKGPGAGETRAGLVFVTSNLSEWVDRDRASTIGAAAAEDPARFDL
                     YRAAFLGHWQDRLGRQRPDGPGRETLGEVLERGSEHRVRSSGA"
     gene            7099..7287
                     /locus_tag="VM841_10265"
     CDS             7099..7287
                     /locus_tag="VM841_10265"
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="HVL90603.1"
                     /translation="MANEIRQPYQAPTVFEFGGLEEITQGALSVNLDDFPVGAKVVCP
                     SGAVCPPPNGGVLPQNPF"
     gene            7361..8680
                     /locus_tag="VM841_10270"
     CDS             7361..8680
                     /locus_tag="VM841_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL90604.1"
                     /translation="MLVQNGSEAEEQTSAFWYRCAGLRIVSVVPLDELARPASVGNPD
                     ISIEEGLPGKIDAIAEESVHVGVVGEVDAPWLEVRRGNGLFLARWPEQLDVVIPDDGR
                     RLIVLQRGPIQDSLARLLLCQGISFALPAHGREALHASAVEIDGRAVVIAGQSGRGKS
                     TLATALCKAGGRLLSDDMACIRIDDLGTPVVEPTSTRAWLADELAEQLTGAGSWQVMH
                     RLHKVSVGDEHIDLAENAVPLAGIFILGYRAGGVEISRPLELKAAMSETLGALFNFAI
                     RTASRMTTQFDVIMPVIEKVPVRTLRWDPSPRNAEPVLDAIRKALDEPAPARPARVPV
                     AAAAAPAPPKSLGTTPTPAAAALRQQVIATLRSAGLDESFDGDDEPLLRTSQVAALLR
                     SSDRTIRTWADAGKLQYIKTLGGRRLFPASAVMAVLQQMRAGYLAEE"
     gene            8683..>10083
                     /locus_tag="VM841_10275"
     CDS             8683..>10083
                     /locus_tag="VM841_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVL90605.1"
                     /translation="MDLQNHPSLDDGINLRETVAVLFRRKLLILFTTFAFFAAAAGMS
                     MQQQKVYSSSANIVIRPVIPPAALQSYFSATGPLGIDFTADTEAQIARSQAVSDLVAK
                     ELQVADPTQLPGGISVKVVTDSLLSFTGSSTDPRTAAVLAQAYATNYLQYRRNQAQAA
                     LETIAIQAKQQVDALSTQIAEMSDQILELSVKAAQETSAAARQAAIAEYNQLNSTRNQ
                     LQFQLASYQSRHSDIQGSMTAASTGGGTIVQQAAIPTVPSSPKPLRDGILGLILGMIV
                     GVGLAFLRQHFDDRIHNLGTASKAVGTLVLGAIPRSTKWRNARHTRLETLEDPSSPAA
                     EAYRSLRQALSTMGVGKELHTVLVTSATPGEGKSTSTANLGVAFARSGLRTIVVSADL
                     QKPRLHAFFGVENRDGLSEVLAGKIWLDNIAPTPVPNLWILPSGAVPSNRGELMDGDM
                     LGHVLAALESVVDVVLI"
BASE COUNT         1502 a         3560 c         3487 g         1534 t
ORIGIN      
        1 ccgcttcccc gaccggttcc cgcttccccg actctgcccg cttccccgga ctccccctat
       61 tgactgattc accccggtaa ccgaatatgt gagaacttcg ggcaaagcat tacaaaaccg
      121 gcacctttcg gggaaaacgg aaccgggatg aggatcagag gacggctcgg catcgtcggt
      181 ctgctgctcg cggcaacgat cgcggccggc ccggcgcccg cgcgtgcggc ctcgcccgcc
      241 accctgctcg acgcgatccg gtccgaggcc gactacatcc tgagcttgcg gaccggcgac
      301 ggcgtcatca ccacggctcc caacagggtg gtcccgtacc tggcgaactt cgccgcgatg
      361 gggctcgcgc gcgcacacct cgttctgcgc gacggccgct acgcgaccgc ggcgtggcgc
      421 tggctcgagt ggtaccgcga ccacatgcac ccggacggca ccatgcccga ctgggtctac
      481 tcgtcgtcgt gggtgccgac cggcgatccc gactcgaccg acgcctacgc gggcacgtat
      541 ttgtcggccg tgctcgccac cttcgaagcc accggcgacc tgctgcggct gcgcgccctg
      601 cacggcggcg tcctcaaagc ggtggccgcg atcgagctga ccgcggacgt ggacggactc
      661 cacttcgcca agccaggctg gccgttcaag tactcgatgg acgaggccga agcatacgcg
      721 ggcttccgcg cggcccagcg gatcgggcgc gcgctcaacg acgcgagcct cgaagcgcgc
      781 gcgaagacga acgcggaccg gctgctgtcg gcttcgatca agctcatcga tccggcgact
      841 gggctgtatc tgtgggcgct gcatcaggac ggcacgcgcg tggtcgcgcc gatcgagtgg
      901 atctatcccg gcgcgagcgc gcaggcgtgg gcggtcgccg acggtctcgc caccggctcc
      961 aacgcgcgga acttgatggc gcgcgtcgag gcggcgcagc cgttctggga ccggccgacg
     1021 gcaaccgcgc agtacgccaa cgcgcagccg aactgcaact tcgccacgcc gtgctacgcg
     1081 cccgtcaact attggccgcg cttcgcgctc gcgtaccttg cgatcggaaa cgcgcggcgc
     1141 gcgatgcagg gcgcgctgag catccacgcc gccgccaaga gcctcggccg cggcttcccc
     1201 tacacgcccg gcgacgcggg ccagatcatc ttgacgctcg ccgacccgca ggcgctcgcc
     1261 gagtcgttga tcccctgaac gccccggcct cgccggcagc ggcacgtcgt gttcgccggc
     1321 cgaggcccgc accagcgcaa gggcgtgccc ggggcccggc caccgctgcc ggtcctgaag
     1381 gcaggggccg aaaagcgaac gaccgcccga aggcggtcgt cgcgtgttct cgaacggctt
     1441 agcgcggcgg gatcggaatg aggtccgggt agcacgccgt cgcgtcgcag ttgccctgcg
     1501 acaggttcat cgcaccgtgg aaggtggcag cgcgggcggt cgtgctcgcg accgacaccc
     1561 ggaacgtctg cgggagcagg ttgccctgcg cgtccctcca agcgaagttg tcggcggtgg
     1621 ttccgttctg cacgcacggg ctcgccggcg ttccccccac aaggctgctg ctggggccgg
     1681 aagccgttag cacgtcggca ccgagaatcc cgaagccgtt gcacgtccac gaggcgacgg
     1741 tctgtccctt cgcgtcgatc caagccatgt tgatcgttcc ggtcgcggcg agatcgatca
     1801 tgtttccgta cgtgccgatg ttcgagatgt agcggaagtc actgatcgtg aacgagaccg
     1861 aacactcggc cgtgccgttc gcagccaccg tgccgctgca ctcaaccggc gtcgatgcgg
     1921 cgtgacccgc cgtcggtacc aaacccccga gcacgaatag gctcgcagcc aatgccatcc
     1981 ttctcacgtc gaacccccaa gactcgagta cctattagcg gtcgccaagc ttctcctctg
     2041 tttttcggcg gcgggggcgt ctttccttcc tgttcaagtc cggaaatggc cccataaacc
     2101 ctcacggagc gggcgggacg agccggattt cgcccccgag agcggtcatt tcggaaagaa
     2161 cggaatattt tccacaagat ttacggaagg agcggaagga acccgtcatc cggacggaga
     2221 accacaaccc cgtccggcgc accatcggcc ggcgggggga aacgctgggg ggagttgggg
     2281 atgttttcgt tcgctcatcc gggcgacgtg gttcgcgcgc tcgagcggag ccgcgactgt
     2341 ttcgatccga gcagcacctc gctcatgatc gtggccgaga ccaagaaccc gcaccgcgac
     2401 ccgttcggcc gtggcttcat cgccggcttc gaggagcgca ccgagctccg caagaggctc
     2461 gcgcgcctgg acgagcgctc gcgcgcgctg gtcatccttt ggcacgccta cgggcacccg
     2521 gtcgcccaga tcgcacgccg cctcggcatc tcccgcgtcc actgctaccg gctcgagcgc
     2581 cgggccctgg acctgatgat cgaccagccc gagggcggct gcgccgaggc cgtcggcgct
     2641 tgaaatagga ttttcccgac ggtcgtcttg acatgtaaca ctttcgtgct ctagcctcta
     2701 aactgataga cgatttggct cttcggatcc gtccggccgt caaatcgagc ggtcctcgcg
     2761 aaccctcgcg ggggcctttt ctttagggat tcttaagcgg ccggcggcca cggcgctgcg
     2821 ccacagaatg acgacagtgg gatatcactt ggacaattca ttcggtatcc acgttcgtgg
     2881 ccttcggcgc aattttggtg ccagttcggt cttggacgga ttgaccatgg acgcgcccag
     2941 cggcgcgctg accgcggtga gtggggcgaa cggagcgggc aagacgacgc tccttcggat
     3001 tctggcgacc ctgctccgcg cggacggcgg cgacgcctgg gtcgccggcg cgcacgtcgg
     3061 ccgcgaaccc gagcgggtcc gcggccgcat cggggtggcg ctggtcaacg accggggcct
     3121 ttattggcgc ctgtcgggat cgcggaacct ggagtttttc ggccgggcgc tcgggctttc
     3181 gcgcgccgac gcccgcaccg acgccgcccg cgtcgccgcc gagatgggca tcgaaggctt
     3241 cgccggccgg ccggtcgcga cctactcggc cgggcagcgc cagcgcctgg tcctggcgcg
     3301 cgcctcgctc gcggacccgg ccgtccttct gatcgacgag ccgatgcgcg gcctggacga
     3361 ggacggcatc gcccgtgccc gcgccatgat gctccgccac gcccgccggg gcgccgccgt
     3421 gctggtcgcg gccccgacga tcgccgagtt ctccgacctg tgcgacaccc gcatcgtgct
     3481 gcgcggcggc cgcgcggagg ccgcatgagc gagaccctgg cgaccgcccc ggcgatcgcc
     3541 gagcgtcgct cccccaccgt ggcctcggcc gccggcctgt tcatgggccg cgagctctcg
     3601 gtcgcgctgt cctacaagac ggcgttcgtg atgaacgccg cctcggtcgc gctgtcgctg
     3661 gtgatgttcc atttcatcgc caagctcgtc accgccacgg ccgcgggcgc caccctgccg
     3721 ggcggctact tctcgttcgt ggtggtcggc cttgcgaccg cgcagatcct cgacggcgcg
     3781 gtcacgcagc cgtcgtcgct ggtccggcaa gagcaggtcc aaggcacgct cgaggttctg
     3841 gccaccacgc cgctgcggcc tgcggcgctg gcggccggat ggatctcgta cccgatcgcg
     3901 gcgtcgctgg tgaactctct gatcatgctg gcgctggccg gcccgctggg cttccggttc
     3961 gaagatccca actggccggt gggcgtgctc gcgctggtgc tgtcgtcgct ggccttcgcc
     4021 ggcttcggaa tcctggtcgc cgcggtcgtg ctggtcatcc agcagggcgc gcagctcacc
     4081 cgctggatct cggccggcat gggactgttg tcgggggtgt tcttcccgat cgagctgttc
     4141 ccccgctggc tccagctcat cgcgcaggcc tcaccgctgt cgcacgccgt ggaggcgctg
     4201 cgcggcagca tgctgcttcg ccgcagcgcc gccgacctag cgccggacct catcgcgctg
     4261 ggcgtgatgg ccgcgctgct cattcctttg tccttgctcg cgttgtccgc cgcgctcgcg
     4321 cgggctcgcg ctcggggaac gatctcgtcc tattaaccgg gagcaaacga atgcgaacac
     4381 ccgctcacat gcccgacgaa gcatccgacg tcgaggttcc caaagaggtt ctgtcccgcg
     4441 agctcgacgg cgaagccgtg ctgctcgacc tgcgatccgg acgctacttc ggtctcaacg
     4501 gcaccggcgc ggtcgtgtgg agcatgctca aggacggagc cgagcgggag cagatcgcgc
     4561 accagatcac cgaagagttc gccgtggacc tggtgcgcgc gcgagccgac gtggacgcgt
     4621 tcatcgagtc gctggttggc cgtggcctca tcacctaccg ggaccgcgcc taaacggggc
     4681 acggccgcgc ggctggctcg cgcggcccgc gacccggccg aaacggcgcg gcgcgtcgcg
     4741 gtgctgggca agatcgtggc ggcgcgcgcg atgatcgcca ccggcgggtt ccggctcgtc
     4801 cgcggcgtgc accgctaccg caccgtcgag caggccgagc ggccgctcgg ccccgagccg
     4861 gccctcacgc ccgcgcaggc gcgcgagatc cagcgggcta tcggattgct ggaccggctc
     4921 gagaagcggg tctcgtgcct tccccgctcg gtggtgctgg agcgggtctg ccgcaaggcg
     4981 ggcgtgccgg ccgagatcgt gatcggcgtg gaccgctcga agggcttcgc cgctcacgcc
     5041 tgggtcgaga tcgagggcta tccgctcggc gcgaccgagg cgggacgcgc ccactggaac
     5101 gcgctcggac ggttccgccg ggacggccga tgagcggctt ttacgccgcg accggcgccg
     5161 ccgccgcgct gccggacggc ctggccggcc ggctggcggg acgcgcggcc ggcgcgatgc
     5221 ggtcctggag cgacgagcag gtcacgctgc tcgccttcga gggccgcagc ggcccgggcc
     5281 tggccgtggc cgacgatctg gtagtggcgc tcgacggccg catcgacgag ccgcgcggca
     5341 ccgcgccggc ggccgacaga cttttgaacg agtggcgccg cgacggcgcg aaggcgctgg
     5401 accggctcgc cggcgacttc gccggcgcgg tctacgaccg gcgcgccacg acactcacgg
     5461 tgctgcgcga cccgtgcgcg ggacggcccg cgatcctcgg gcaggtcccc ggcgggctgg
     5521 tcgcggcgag caccgtgccg atgctgctgg aggatccgcg cctcgatcgc gagatcgatc
     5581 cggaatgggt cgcggcctac ctgaccgcga gcgcaggctt cgcgaccgcg accggatacc
     5641 gggcgatgac ccggctgatc ccgggcgccg cggccgaggc gcgcggacgc gactggacga
     5701 tcgtgcgccg tcacacctgg cggcctgcgg ccgtgcggga acgaaccgat caggcctatg
     5761 ccgacgggct gcgcgagcgg ctcgtccgcg cgatcgagga ccggaccgcc ggcgcgagcc
     5821 ggatcgggat ctcgctgtcg ggcgggctgg actcgacgag catcgcggcg ctgctgcgcc
     5881 gggcacggcc cgacgcgcag atcgtggcgc tcgcagtgcc gttcacgcat ccgcggggcg
     5941 acgagcgggc cttgcagaag atggtcgccg agcgaatcgg cgccgagctt cgctgggcgc
     6001 cgctggacgg gaccagcccc tacggggcgt ccgcagccgc cacgctcgac cggctcgggc
     6061 agccgccgat cgcgccgaac cacttcttcg tcgagcgggt cgccgccgaa ggacgcgccg
     6121 ccggggtcga ggtcgcgatg gacggcatcg acggcgacgc ggtcgtcggc ggcaactggt
     6181 tctacctcgc ggacctgctg gtgaccggcc ggctgggggc gttccggcgc gagctcgcgg
     6241 ccgcggcccg gatccacggg gtggcccgca agcggatcgt caaggactat gtggcggcga
     6301 cgctgctgcc ggaccggctg cgcgccctgc ccgcggtccc cgactactgg gacccggcct
     6361 tcgtgcgcaa gcaccggctg gccgagcggc tccgcggccg gagccggggg cgtccgggca
     6421 gggcgttccg ggccaacgag ctggccgcga tggcgccgga cgtggcgccc gcgatcctgg
     6481 agcagatcgg cctggcctgg ggcccggtcg aggtgtccca cccgttcctg gaccgccggg
     6541 tcgtcgagta ctgcctgggc ctccccagaa agcagaaggt ctcggcagga gtgaccaaga
     6601 tcgtgctccg aaacgccctc cgtggcctgc tcccgccgga agtagcggag cgttcggaga
     6661 aagcggaact cggaaaagca ttctttgagg gtctaaaagg gcccggagca ggcgaaaccc
     6721 gtgcaggact cgtttttgtg acgtcgaatc tgtctgagtg ggtagaccgt gatcgtgcat
     6781 cgaccatcgg tgcggcggct gcggaagacc cggcgaggtt cgacctgtac cgcgcggcgt
     6841 tcctcggaca ctggcaggac cggctgggcc ggcagcgacc ggacgggccg ggaagggaaa
     6901 cgttggggga ggtacttgag cgtggatccg agcaccgcgt acgaagctcc ggcgcttgaa
     6961 gatttcgggg agcctcgtga gatctcgctc agcagggccg aggaagcgtc gaggccggta
     7021 atcgacgcgc tcgtcgcgga acgagtggca acgaacgtgg taggaccggg ccgacgaacg
     7081 aacacggggg cataacttat ggcgaacgag attaggcagc cgtaccaggc gccgacggtt
     7141 ttcgaattcg ggggtctcga agagatcacg cagggtgccc tctcggtcaa cctcgacgac
     7201 tttcccgtcg gcgcgaaggt cgtgtgtccg agcggtgccg tgtgcccgcc accgaacggt
     7261 ggcgtgctcc cgcagaaccc gttctagacc acacagacaa cgacttttca agagaagacc
     7321 cgcctgaggg cgggtcttcc gctatctgga ggaagaatcc gtgctggtac agaacggctc
     7381 cgaagccgaa gagcagacat cggccttttg gtatcgatgc gccggcctgc ggatcgtgtc
     7441 ggtcgtcccg ctcgacgagc tggcccggcc ggcgtcggtc ggcaaccccg acatctcgat
     7501 cgaggagggc ctgcccggca agatcgacgc gatcgccgag gagagcgtgc acgtcggcgt
     7561 ggtcggcgag gtcgacgctc cctggctcga ggtccggcgc ggcaacggcc tcttcctggc
     7621 gcgctggccc gagcagctcg acgtggtgat ccccgacgac gggcgccgcc tgatcgtgct
     7681 gcagcgcgga cccatccagg actcgctcgc gcggctgctg ctgtgccaag ggatctcgtt
     7741 cgcgctgccg gcccacggcc gggaggcgct gcacgcctcg gccgtcgaga tcgacggccg
     7801 cgcggtcgtg atcgccggcc agtcgggccg cggcaagtcc acgctcgcga cggcgctgtg
     7861 caaggccggc gggcggctgc tgtccgacga catggcctgc atccgcatcg acgatctcgg
     7921 gaccccggtg gtcgagccga ccagcacccg cgcctggctc gccgacgagc tcgccgagca
     7981 gctcaccggc gccggctcct ggcaggtcat gcaccggctg cacaaggtgt cggtcggcga
     8041 cgagcacatc gacctcgccg aaaacgccgt gccgctcgcc gggatcttca tcctgggcta
     8101 ccgcgcgggc ggcgtcgaga tatcgcggcc cctcgagctg aaggccgcga tgagcgagac
     8161 gctcggcgcg ttgttcaact tcgcgatccg caccgcttcg cgcatgacca cgcagttcga
     8221 cgtgatcatg cccgttatcg agaaggtacc ggtccggacg ctgcgctggg atccgtcgcc
     8281 gcgcaacgcc gagccggtgc tcgacgcgat ccgcaaggcg ctcgacgagc ccgcgccggc
     8341 caggcccgcg cgcgtgcccg ttgctgcggc cgcggctccc gccccgccca agagcctcgg
     8401 cacgacgccg accccggccg ccgcggcgct tcgacagcag gtcatcgcga cgctgcgcag
     8461 cgccgggctc gacgagtcct tcgacggcga cgacgagccg ctgctgcgaa cctcacaggt
     8521 cgcggcgctg ctgcgctcga gcgatcgcac catccgcacg tgggcggatg caggaaagct
     8581 tcagtacatc aagaccctcg gcgggcgccg gctgttcccg gcctctgccg tcatggccgt
     8641 tctccagcag atgcgcgctg gctacctcgc agaggagtaa cgatggacct tcagaaccac
     8701 ccgtccctcg acgacggcat caaccttcgt gagaccgtcg ccgttctgtt cagacgaaag
     8761 ctgctgatct tgttcaccac gttcgcgttc ttcgcggccg cggcgggcat gtcgatgcag
     8821 cagcagaagg tgtactcgtc gtcggcgaac atcgtgatcc gtccggtgat ccctccggcg
     8881 gcgttgcagt cgtacttctc ggccaccggc ccgctcggca tcgacttcac cgccgacacc
     8941 gaggcacaaa tcgcgcgcag ccaggccgtg tcggatctgg tcgccaagga gctgcaggtc
     9001 gcggatccga cgcagcttcc cggcgggatc agcgtcaagg tcgtgaccga cagcctgctg
     9061 agcttcacgg gctcatccac cgacccgcgc acggcggccg tgctcgcgca ggcgtatgcg
     9121 acgaactacc tgcagtaccg ccgcaaccag gcgcaggccg cgctcgagac gatcgcgatc
     9181 caggccaagc agcaggttga cgcgctgagc acgcagatcg cggagatgag cgaccagatc
     9241 ctggagctgt cggtcaaggc ggcacaggaa accagcgccg ccgctcgtca ggccgcgatc
     9301 gccgagtaca accagctcaa cagcacccgc aaccagctgc agttccagct cgccagctac
     9361 cagtcgcgcc actcagacat ccagggcagc atgaccgccg catcgaccgg cggtggcacc
     9421 atcgtgcagc aggcggcgat ccccaccgtt ccgtcgtcgc cgaagccgct gcgtgacggg
     9481 atcctgggcc tgatcctcgg catgatcgtc ggggtcggcc tcgcgttcct tcgccagcac
     9541 ttcgacgacc gcatccacaa cctcggcacc gcgtccaagg ccgtcggcac gctggtgctg
     9601 ggagcgattc cgcgcagcac caagtggcgc aacgcgcggc acacgcgcct ggagacgctc
     9661 gaggatccga gcagcccggc cgcggaggcg taccggtcgc tgcggcaggc gctctcgacg
     9721 atgggcgtcg gcaaggagct ccacaccgtg ctcgtgacct cggcgacgcc cggcgagggc
     9781 aagtccacct cgaccgcgaa cctcggcgtc gccttcgcac gaagcgggct tcgcaccatc
     9841 gtcgtctcgg ccgatcttca gaagccgcgt ctgcacgcat tcttcggtgt cgagaaccgc
     9901 gacggtctca gcgaggtgct cgccggcaag atctggctcg acaacatcgc gcccacgccg
     9961 gtgccgaacc tgtggatcct gccgtcgggc gcggtgccgt ccaaccgcgg cgagctgatg
    10021 gacggcgaca tgctcggcca cgtgctcgcc gcgctggaga gcgtcgtaga cgtcgtgctg
    10081 atc
//
LOCUS       DATLFO010000254        27972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88031, whole genome shotgun sequence.
ACCESSION   DATLFO010000254 DATLFO010000000
VERSION     DATLFO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88031"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="rapZ"
                     /locus_tag="VM841_10280"
     CDS             <1..645
                     /gene="rapZ"
                     /locus_tag="VM841_10280"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HVL90606.1"
                     /translation="EAALKDLGARGLDYRIVFLTADDEAIVRRFEATRRRHPLADRVV
                     DGIKMERAQLESLRAQADLVIDSSNLSPHDLRDRIAGVFSSKPREARLRITVVSFGYK
                     HGVPLDADIVMDVRFLPNPHWVDELRPLTGVDAPVRDYVMSREATQAFLSRFEDLLGV
                     LIPGYLAEGKHYLTLGIGCTGGRHRSVVVAEEVAELLRDRGLQVAVEHRDSHRA"
     gene            638..1522
                     /gene="yvcK"
                     /locus_tag="VM841_10285"
     CDS             638..1522
                     /gene="yvcK"
                     /locus_tag="VM841_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HVL90607.1"
                     /translation="MPDDARIVCIGGGHGLSAALSAAKQLTPDVTAVVTGADDGGSSG
                     VLRRWLGIPAPGDLRMAIAALAGEDQAALMQYRFSEGDLAGHPLGNLLIAALADVRGD
                     FVRAVAEAARIAGVTGAVYPASPDPLTLHATIGGNAVEGQVAIALGPDAVERLWLDPA
                     EPPATPEAVEAIERADLVVLGPGSLFTSILAALIVPGIGRAVRNARRVAFVLNLSEQL
                     GETKGMSAAAHIDAMTGHVRRLRLDVVLVHEGPSGGMRRPVHLDEQSIRVPIVKADLV
                     SDGVHDPLKLAATFKTLL"
     gene            1519..2442
                     /gene="whiA"
                     /locus_tag="VM841_10290"
     CDS             1519..2442
                     /gene="whiA"
                     /locus_tag="VM841_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HVL90608.1"
                     /translation="MKSPRAASFSRRVKEELAHIRAPRECCRRSEMVGMVRGGGTLHI
                     LPGGRYALEVEAPDAAVARAVYSALSVLGGLPEVRQYSPGRAHPHERYVVRIESGEAS
                     LFSRAGALAADGLPATGVPRGVFERRCCAGAYLRAAFIVHGTVSPPRSATHLEIRAPD
                     AGTAEGLTELGHMVGARLRVRDHRGHAVYTKDVSSVGALLAAMGAHQGCLEWEAGGVW
                     ATVRNEANRLANCDQANVRRTVRASLQQRDAIRRLDAEGRLDGLPSGLRDAARVRLEH
                     PDASLDELAKELRISKSAMADRLRRLTALAG"
     gene            2513..3517
                     /gene="gap"
                     /locus_tag="VM841_10295"
     CDS             2513..3517
                     /gene="gap"
                     /locus_tag="VM841_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVL90609.1"
                     /translation="MAIKVGINGFGRIGRNFYRAVAKQGADIEIVGVNDITDPATLAH
                     LLKYDSVHGRLDAEVKPTGDGISVSGKEFRVTAERDPANLPWKELGAEIVIESTGLFT
                     DRDKAAKHMDAGAKKVIISAPAKGEDGTFVIGVNAHEYDPAKHHIISNASCTTNCVVP
                     MAKVLRDAFGIEKGLMTTVHAYTNDQNLLDLAHKDLRRARASAVNIVPSSTGAAKAAS
                     LAIPELKGKLDGFAMRVPVPDGSVTDLVVILEQEVTAADINAAFQAAASSGPLAGVLE
                     YTEDPIVSSDIVGNPHSCIFDSKLTMANGSLVKVVGWYDNEWGYSNRLVDLVQLVASK
                     "
     gene            3526..4674
                     /locus_tag="VM841_10300"
     CDS             3526..4674
                     /locus_tag="VM841_10300"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVL90610.1"
                     /translation="MRTIDDLDVAGKRVLVRADFNVPLNGGAIADDFRIRAALPTLIE
                     LRERGAAQLVLCSHLGRPKGAPDPKYSLAPVAARLSELLGAGVALAPNPDGPVPAGAP
                     VVLLENLRFHPGETTNDAAFAKALAAMADVYVNDAFGAAHRAHASVAHVATELPAAAG
                     RLLEKEIAVLSGLLESPERTFVAVLGGAKVSDKLKVIENLLGVVDRLLIGGAMCFTFY
                     AAQGLEIGKSLFEPDQVETVKRLMAEAGDKLMIPADVVVAREPKAGVTATEVKADAIP
                     ADQAGYDIGPEACRAYVDAIRGAATVFWNGPMGIFEVPPFDKGTRAVAAAIAKGGAYS
                     VVGGGDSALALDRFGYADGIDHVSTGGGASLELLEGRDLPGLVPLWVR"
     gene            4764..5210
                     /locus_tag="VM841_10305"
     CDS             4764..5210
                     /locus_tag="VM841_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90611.1"
                     /translation="MPVRRSILAVLLVLGVAPAWSSPGEVDIRASTSEAGSGSVGECG
                     GFAPSSPSCSFTGNLPAASTVHLSGQLEFTGRIRIVLKTSTGEYMASCDFLGVFDPVC
                     RSNTSGVLQIGQSFTLTATASAYNPHDRRDLGDAAVGRWRVSMTAN"
     gene            5297..6067
                     /gene="tpiA"
                     /locus_tag="VM841_10310"
     CDS             5297..6067
                     /gene="tpiA"
                     /locus_tag="VM841_10310"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVL90612.1"
                     /translation="MTRKPLLAGNWKMHKTHLEAIAIVQKVHYLLRPQDLDAADALVC
                     PPFTALRAVQTIIESDDMRIALGGQNCHWEKEGAFTGEVSAPMLARLKCSYVIAGHSE
                     RRAYFGESDEIVNKKAKAIFAAGMTPIVCVGEQLDDREAGRAQDVVGAQLKGSLKGLG
                     AEQLGAMVIAYEPVWAIGTGKNATPQDAQDMCAFIRGEVAKLGGQPAAGTVRIQYGGS
                     VKAGNIASIMEGPDVDGALVGGASLDPEEFALIVRYQG"
     gene            6064..7356
                     /locus_tag="VM841_10315"
     CDS             6064..7356
                     /locus_tag="VM841_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVL90613.1"
                     /translation="MKKVLYVVLDGLGDDPLPELDNRTPLEAAETPWMDRLARDGANG
                     LVYTVGKGIAPESDIAVFAVLGYDPREHHSGRGPMEALGSGMDVRDGDLVYRINFATV
                     EPGDGPNWTILDRRVGRDLTSEEAHALAAEVNANVKLPGATAELLATVEHRGALLIRR
                     EGKALSAESTNTDPAYGREGALGVAKLTFDNHVQACEPLPGHEGDEAAVAGADLTNEF
                     LRQAHAILEASEVNAKRRNAGKLAGNIILTRDGGDHLPHPPPFKERYGAQWGCFVEMP
                     VERGISMVLGMGQVEVPMKGSGTHGEQYATWAETALEAIQEYDGLYIHIKGPDVPAHD
                     GRGQDKRDVIAEIDESFFATIVPELGKDVIVAVTADHATSCAKKSHTDDPVPLLVWGP
                     GVSEDAVQTFSETAAAKGSLGLLQGVDIMPRLMELARE"
     gene            7551..9173
                     /locus_tag="VM841_10320"
     CDS             7551..9173
                     /locus_tag="VM841_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90614.1"
                     /translation="MQKKRMFQLFAGALVLSLVAAACGGDGNGGGSGSGDGKAGGSAI
                     AELGEPDGLAPHLCGTTSCSEPANRIFDSLLEYDWKTAKVVTTGAATSYEVSPDGTKI
                     TYQLRKGATFHNGEPVDAASFIRSLTLTASKDAASDVAYHLGGIKGFKDLNEGTAKDF
                     PGVKQGKDEYELILELDQPNAEFFVRTGHMVFAPIPKVALAADGTFNKDYNEAPIGNG
                     PYMVDGTWQHNVSLKLKKYADYNGPVKGFLDTIEFKIFEKLETAFLEFQGGTLDTTPV
                     TPDQFDAAEAQYGDAFIEQATAVLTYLLANNAKAPTNNADFRRALSLAIDREEIVKAV
                     FSNRRIPAKSIVPPASPGHRPDACKYCTFDLDKAKEFLQKAGGPKNINLSFNSGAGHE
                     EWTAAVASQLEKNLGVKAQLIGKTPFGDYIKYLDTDEWGGGLGRLGWAQDYPTPDNWL
                     FPLLHSQSGDNHSKYKNPEFDKTIEESQRTLDAKQRLALQQKAEDIALEDMGFIPMFY
                     GKSARVFNKDKFASFPLELQSGNPAWEEVSIK"
     gene            9252..10184
                     /locus_tag="VM841_10325"
     CDS             9252..10184
                     /locus_tag="VM841_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016934609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL90615.1"
                     /translation="MNRYIARRLLQFIPVLLGTLTLLFVALFILPGDPVQALVGERPV
                     SEGFRQAIIDKYGLDDPLHVQYGRYLKRLVQLDFGESAASRRDVWDIIKERVPVTARL
                     AVAATFYLLILGIGSGVISAVRRYGISDAAVTVGTIVLVSIPVFVLGLLLQIFALKTK
                     DVLGLPISGLNAGLKSYILPGFALASVSSAYISRLQRTALLETLGTDYVRTARSKGLR
                     ERRVVMNHAWRNALIPVVTYLGLDIGTLLTGAVLTETVFNIPGLGRALAQGVTQQDNQ
                     VVLGVSVFIVLVYLVANLIVDILYAVLDPRIRYQ"
     gene            10206..11141
                     /locus_tag="VM841_10330"
     CDS             10206..11141
                     /locus_tag="VM841_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL90616.1"
                     /translation="MTTTKTDPDQPREPLSSDVPDPTAVPDGLLEVTSLRGEFWRRLR
                     KNKLAVAGMIILGLIIFAAVFANVIARQPTAGFFPSEVREGPSWRHWFGTDLLGRDIF
                     ARTVHGARFSLIVGASVVSVSLLIGLTIGGLAGYFGGWIDTLLSRLIDVWLGFPYLVG
                     ALIIVVSLGGGKFALVAALSTFGWVTIARLFRGSVIAIRDSEYVEAARALGAGNGRIL
                     LRHVLPNAVTPTLVYAFALVGTTIISEAALSFLGFGVQEPETSWGLMIARSQSQMLAG
                     HAHMVLFPSLALTMTVLGFILLGDGLRDSLDPRLR"
     gene            11144..12265
                     /locus_tag="VM841_10335"
     CDS             11144..12265
                     /locus_tag="VM841_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90617.1"
                     /translation="MSDVRVETTPTTPPGEHLLEVEDLAVSFFTDTGVVTAVDGVSFS
                     LDAGRTLAIVGESGSGKTVSTQAMMGLIPKPPGKIMRGTAKFRGRDLLTISDEEMRKI
                     RGREIAMIFQEPLTALNPVHRVGSQIVEMIRSHEEVSKKEARQRAIELLKTVGIPQPD
                     KRVDNYPHEFSGGMRQRAMIAMALALNPAILIADEPTTALDVTVQAQILELLKGLQEE
                     FGMGLIMITHDLGVVAQISDEILVMYAGRMVERGDPEDVFYKPQHPYTWGLLGSIPRV
                     LEEELTDERLVNIPGMPPSLQRVPPGCPFHPRCTFRFEPCDTERPELLQRGHHEDACF
                     LEQDEKEAGRADILHLGTKPTPKPKSKPPSVARKAGTRS"
     gene            12271..13548
                     /locus_tag="VM841_10340"
     CDS             12271..13548
                     /locus_tag="VM841_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HVL90618.1"
                     /translation="MATTPQAAPSRSGTPLVEVRNLKKYFPILEGIVFKKEVGRVHAV
                     DDVSFDIYEGETLGLVGETGCGKTTTGRLILRLLEPTSGTIKFEGEDISEFDTNALRP
                     LRRKMQIIFQDPFASLDPRMTVGSIIGEPLDIHRLADGDQKLRRTQELMEMVGLNPDR
                     HNRYPHEFSGGMRQRIGVARALAVEPKLIVCDEPVSALDVSIQAQILNLLDDLQKELG
                     LTYLFIAHDLSVVRHVSDRVAVMYLGKIVEIATGRELYTDTRHPYSGALLSAIPKADP
                     VVARSTKRILLTGDVPSPVNPPSGCRFHPRCPVANERPEERLPVCSTQEPPLASMDGD
                     HVAACHFPLPPKTGADLSGMNLAGGAPRDDEVPVKPKTARKTSPRKTTGKTTARKAAS
                     GARTASRAGKKTAAKPAGKTTSSRARKSPPGAR"
     gene            13560..15347
                     /locus_tag="VM841_10345"
     CDS             13560..15347
                     /locus_tag="VM841_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90619.1"
                     /translation="MAFTTAHPRRGLRVLALACVLALAAGACTQGADGESRPGETPEP
                     GGTLRIAAGGDVDSLDPGRTYTTFGFMLFRVMVRTLVSYAPKPGEAGNKLVPDLAEDT
                     PTVSPDGLTYTFVLRPGVRYQPEIAAGREVRSEDFRYAIERGFYASVSNPYADLYFGD
                     VFAGDEKFIAAPSHKTHIDGIDVSDPRKLVLRLKRPVGDLLWRLALPLAAPVPPEYAI
                     PLDGGTESSYGDAFASSGPYQIERQNATVTGYVPGKAIRMVRNPNWDARTDPIRKAYP
                     DRIEVTGGFADLAAATGKILAGEMDYNGDFPIPPEQQKDILSSASTKDLIHYNATSCM
                     RYVSMNATIPPFDNPLVRKAVNYALDKRGMRAVRGGERTGSIATHVLLPGLPGFKEVG
                     QKDYDPYSSRDFAGDVEKARDLMREAGYPTGLYTGPSVVMAGEASEIERRTGDVIVSS
                     LEKIGIKVRREEHKRPALLARMGVQKSGIAIGASAAWCWDYPDAYTVIAPLFDGRRIR
                     EKGNTNYSQINSPSLNELIDKASGASGDDRAAAWAAADKFVMDLAPVVPWLWDSSPHL
                     VSPRVVGYQFALSSVSMDLAVAAVRQDQE"
     gene            15373..17154
                     /locus_tag="VM841_10350"
     CDS             15373..17154
                     /locus_tag="VM841_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90620.1"
                     /translation="MRTRFGGARAGAAVLVLALVGAACGADAPAEEGPARGGTFKIAA
                     AGDIDSFDPGKTTGTMGSLLMRVLVRTLVAYPAVDGETGIEPVADIASSEPAISPDGK
                     RVVISLRPDVKYQPEVAGGRAVQSRDIRYAIERGFYPSVANPYARTYFAGIFVGDEAF
                     LAAPGPDKNIAGIDVSDPARIVFDLKAPVGDLLNRLALPMAAPVPEEYAKQFDVKPVS
                     DYGANVAATGPYQLERSGSTVTGHKPGESITLVRNESWTASSDPIRKAYPDKIEVTEG
                     LDEITRTVDRVLSGEFDYAADIGLPADRAAEILNDDTAKTQLFFNAGNCMRFISFNTT
                     VKPFDQLKVRQAIATLINKGDLLEARGGKRTGDVAGHVLMPGMPGFLEAGGRTYDPFA
                     RAVEGSTLVRAQEMMKEAGYTTGQYDPEKKERPVLVVGIDEDFHRRITDIVVKALTDL
                     GIRVDRQDYKASDVSGRFASVPRSNVAVLPNAAWCWLYPDAATVIPPLFDGRKIAPAG
                     NTNFSLLNDPEVNALIDQALAARGKERADLWSRADRLIMDRVPVLPWLWERVPTLISP
                     RVVNFQYLLPTTSLDLAVVAVKQAPAA"
     gene            17151..18848
                     /locus_tag="VM841_10355"
     CDS             17151..18848
                     /locus_tag="VM841_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90621.1"
                     /translation="MTGRRAIAAACICAAAFAACTGSPGPGPGDGDRPPGATRGGTLT
                     FALADDAGVLDPQLASQPGAFALIRATQRGLMAFPSSEDPAEAAVPVPDLAADEPDVS
                     ADGRTVTFTLRRGPSFGPPASRPVEAADVKAGIERLFVTRSPLATYFRVIVGAADFEA
                     RRAPSIAGIETPDARTIRFSLTAPANDLTWMLAHTAASAVPAGTPAAVGPKDLASSGP
                     YRVERVAAGREIRLVRNDAWAAGSDPVRGAYVDEIVVRVGSAATADLTGDEVPVPAGA
                     EGRGVDAGCLLYLFVRPQGPFAERNARAAISLAIDRRALVEEVARATGTFSATATVSL
                     LPPMLAGATERPLPPGDAAEARRLLAGASPQVAFGREASGPVAAQDKAAADVISRMLA
                     AAGIRTVPLAVRSPGSIYEVYGRGAAPMGLARWCPDWPGRGARTMIAALAGTGAVANY
                     SGTSDATLDALVTAAVAETDPGRIAAQTAAAADRMLALVTIVPLAFLSDRVAVSPRVR
                     GFVPHPFFVRGDPTNVWLEELAPSPAPSPTAPVPAGGASSIAVRYTSRVPTTNGGVS"
     gene            18845..19072
                     /gene="secG"
                     /locus_tag="VM841_10360"
     CDS             18845..19072
                     /gene="secG"
                     /locus_tag="VM841_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVL90622.1"
                     /translation="MTPVILGFQILSACLLILFILLHAGRGGGLSDMFGGGMHAAGGS
                     TVVEKNLDRITVITAIVFTISTVALHFRIRP"
     gene            19083..20675
                     /locus_tag="VM841_10365"
     CDS             19083..20675
                     /locus_tag="VM841_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HVL90623.1"
                     /translation="MSVLPGRMRRTAAAALALAALLVSCSEDAPSREPENPVAGGTLR
                     VSVRELNSLDPAKARGQGAALVLEQVFDSLTGVDDAGNAVPAAAEKWTTSPDGLTWTF
                     TLGRSVFHNGDPVTAYDFKFAFDRIAGKTLASEAAFQLEPVRGFKAVRIDGTTQALSG
                     VEVVNASTLRITLDRPFYELPVYLSHAALGPVSQKLLNRDPAAFAKHPIGNGAFEVAA
                     PMSVAGVRLTRFDGHAGATAYLDALDVVIHEGADDGYRAYLRDEVEIAEVPNSAIESS
                     RGRIGPSGFTPLWAAVYYGPNMRLDKFKNPEFRRAISLAINRATIASSVYGGTKMPAT
                     GVIPLGIPGFPPDRCRDCRFNPGSARDLIKAAFPSGPPEVVLDHLAASPSREVAAAIA
                     ANLREVGLTVSLRAHESGAYLRLLESGQQELAELGWLAEVPSSDGFLAQQLRSGSPNN
                     QVGFSDSAFDTAIDKARATKGIAERRAQYQSAETRALAEMALIPVVFFRNHLAVARSV
                     RGFDLNGAGLFDASRVWLSTEP"
     gene            20654..22252
                     /locus_tag="VM841_10370"
     CDS             20654..22252
                     /locus_tag="VM841_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90624.1"
                     /translation="MALDRAVTAAARRRIRVAGALAAAALLLTACPAGEPKRPAGSPT
                     ATPLPTTPPEGEIRLAWPHEPATLNPFVRGGESPSARALLRPLWPPLFAAGPGGEATP
                     WLVDRVLSEEPLSVRVALRADAVWSDGEPIKASDVVFTWKTIVDPASKAIAPAGYDAI
                     ASVTAESQTIVAITFKHAVPAWTDLFSAGLGVLPEHALAGKGIGEIGAWPVSGGPFVL
                     ERYTRGLEMVLRATPQPWRVRARVRTIRVMFVPDSTTAIQLLRAGRVDVLGPYSSPDF
                     SRRLTAEDGVAVSTDTGAVWTGLVLNTASSILSDARVRQAAAFAVDRAAIVEGLVRGE
                     GGGLGRPFVYPEAGSEGVPYDPQRAAQLLAEAGFAERRGGTRAKGGTPLEISIALSGG
                     DDLAQRVVRAVFAHLNAAGFAVNLVSLEDEALWRDWLPSSRFQAALVTFADPPGGALR
                     DRYHSAEVMPGGLNVPRVGDPALDAGIEAGDAARVAAELERLAPVVPLWRASVPAAAR
                     GGVQGVRAVASADGMLVLAHDWAI"
     gene            22320..22403
                     /locus_tag="VM841_10375"
     tRNA            22320..22403
                     /locus_tag="VM841_10375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22354..22356,aa:Leu,seq:gag)
     gene            22733..23479
                     /locus_tag="VM841_10380"
     CDS             22733..23479
                     /locus_tag="VM841_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL90625.1"
                     /translation="MTVRRAFLVGIPVGLAGVIALLAIEVRLARRAERIEPFDRSVLD
                     GSIGGESASPLRIAWLGDSTGAGVGASSPDNALPRVVARGLRRPVDLTVLAESGARVR
                     DVLDDQIPLLQSLRPDWVIVGIGGNDVTHLTSRASFRSDLADVLRRSAAVGAERVVLV
                     GIGEFAATPLLAQPLRAIAGMRADRLAADQKRVARERGALFVDVIEATGAAFVRDPKR
                     YHAHDGFHPSDDGYALWADAVLSTIEATRG"
     gene            23645..24652
                     /locus_tag="VM841_10385"
     CDS             23645..24652
                     /locus_tag="VM841_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVL90626.1"
                     /translation="MKLRTVAAALALAVLPASGLAPAAAATDCTDPSATMTALPAAAG
                     AGTGGYVRPAAAPTALVVFAHGYTHESASWEQHMQDAAAHGALAVTPDYSYSIFKRGW
                     HVSAGAQDMIAAANYFLDLCGATIRETILFGVSMGANTSGLALAEKPMRGGAPLFDVW
                     FDVEGAVNVIETYAGASALAGVNAFAANAKADIEAETGGTFSQVPEEYAKRAVVNRVG
                     DIRDSGVKGVVLVHGVEDGLVPYNQSREIVALLRAHGVPADMTTVVRKGSGENGTTLS
                     GYAAQGSPFAGHGSESSATQRVIRIAHDRLHAMLDEIAANNEPAVEDRERIVDDADTI
                     E"
     gene            complement(24653..26011)
                     /locus_tag="VM841_10390"
     CDS             complement(24653..26011)
                     /locus_tag="VM841_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL90627.1"
                     /translation="MPASTPAAALQAAAQAKTLAMERSVSILRAAVVGVNVVVYLLFM
                     QGNTPRPALAWTIIAIAVPYAIWSLVFRPYERYPLLRFGAATIVSDSILIALWILGTG
                     GAASDFWVVYVVSAVSVGMRYDLATSLLVGAAESALYLAMMTMDGGLPGAAAILRPGY
                     IVIAAGAAGLVARQERTTREERVLFERLASEHAEMLSRERQTVEALREVDQMKTTFIS
                     AISHEVRTPLTAVLGFAKTLMSRDHVLTVEEKAEILHRIVASGERLERILMDLLDLNA
                     YTNGVAQLHRGPTRVADVVQRVLDEVAVGDHPIEVDVPGGLTAAVDAPKVERIVSNLV
                     RNAVKYTPSGLPITVRACRMNGGTLIIVDDRGEGIPDDHKAAIFQVFRQGPQRVEHAP
                     GTGIGLALVARLSELHGGRAWVEDRADGGASFRVMLPDGAPGARTGPGMSGAALSDAA
                     SL"
     gene            26302..26760
                     /locus_tag="VM841_10395"
     CDS             26302..26760
                     /locus_tag="VM841_10395"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HVL90628.1"
                     /translation="MYERPTKVCLVWGAAEGDSELTAFDNALLKAGIGHLNLMAVTSI
                     FPEGAALSTLPEIPTGTLTPTVYAKMVSETPGEVISSCIGVGLTDRGGVLMEAHGPWT
                     AQQIEERVTKMVAEGMAVRGFGDYELHFATAEHRVDRIGSAVAAAVLWRG"
     gene            26767..27111
                     /gene="speD"
                     /locus_tag="VM841_10400"
     CDS             26767..27111
                     /gene="speD"
                     /locus_tag="VM841_10400"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HVL90629.1"
                     /translation="MNALGRHFLVELWEATNLDQPAVIEEALMEAVRVSGGTLLDTKI
                     VPFPNGACSGIAIIAESHLSIHTWPEFGYAAVDMFTCNHDMDADAGFDVLRKYFSPGR
                     IQVVEVKRGLSA"
     gene            27108..27965
                     /gene="speE"
                     /locus_tag="VM841_10405"
     CDS             27108..27965
                     /gene="speE"
                     /locus_tag="VM841_10405"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HVL90630.1"
                     /translation="MTRFHEEPAPGLEQAYQGTLLHAEESAFQKIEVYEHPTFGRMLV
                     LDGLVQTTTRDEFCYHEMLVHVPMIAVAEPRRVLIIGGGDGGTLRRVLEHATVTEAVM
                     VEIDETVTTVSREFFPEIAGDAFDDPRATVLFDDGAAYVRDGSEPFDAIVIDSSDPVG
                     PGVVLFSEEFYGQCRVRLNPGGVLSAQIGSPFYFPDEIAMALGNAAKAFPQVKPYVGA
                     VPTYPGTLWGYMLAGERIDTDADTAGKRAAERGLQPRYWTPEIQAGAFAVPAFVAEVA
                     SGSPVFPKP"
BASE COUNT         4373 a         9830 c         9485 g         4284 t
ORIGIN      
        1 gaggccgcgc tcaaggatct cggcgcgcgc gggctcgact atcgcatcgt gttcctcacc
       61 gccgacgacg aggcgatcgt gcgccgcttc gaggcgaccc gccgccgtca cccgctcgcc
      121 gaccgcgtcg tggacggcat caagatggag cgcgcccagc tcgagtcgct ccgcgcccaa
      181 gccgatctgg tcatcgactc cagcaatctg tcgccgcacg acctgcgcga ccggatcgcc
      241 ggggtgttct ccagcaagcc acgcgaggcc cggctgcgca tcacggtcgt gtctttcggc
      301 tacaagcacg gcgtgccgct cgacgccgac atcgtgatgg acgtgcgctt cctgcccaac
      361 ccccactggg tggacgagct gcggccgctg accggcgtgg acgcgcccgt ccgcgactac
      421 gtgatgagcc gcgaggcgac gcaggccttc ctgagccgat tcgaggacct gctcggcgtg
      481 ttgatcccgg gctacctcgc cgaaggcaag cattatctga cgctgggcat cggatgcacc
      541 ggcggccgcc atcgcagcgt ggtcgtcgcc gaggaggtcg cagagctgct ccgcgaccgc
      601 ggccttcagg tcgcggtcga gcatcgggat tcgcatcgtg cctgacgacg cgcgcatcgt
      661 gtgcatcggc ggcggccacg gcctgtctgc cgcgctcagc gccgccaagc agctcactcc
      721 cgacgtcacc gccgtagtca ccggcgccga cgacggcggc tcgtccggcg tgctgcgccg
      781 ctggctcggc atccccgcgc ccggcgatct gcgcatggcg atcgcggcgc tggcgggcga
      841 ggatcaggcc gcgctgatgc agtaccgctt cagcgaaggc gacctcgccg gacatccgct
      901 cggcaacctg ctcatcgccg cgctggccga cgtacgcggc gacttcgtgc gcgcggtcgc
      961 ggaggccgca cggatcgccg gcgtgaccgg cgccgtctat ccggcgagcc ctgacccgct
     1021 cacgctccac gcgaccatcg gcggcaacgc cgtcgaaggc caggtcgcga tcgcgctcgg
     1081 cccggatgcg gtcgagcggc tctggctcga tcccgccgag ccaccggcga cgcccgaggc
     1141 cgtcgaggcg atcgagcgcg ccgacctggt cgtgctcgga ccgggcagcc tgttcaccag
     1201 catcctggcc gcgctcatcg ttccgggcat cggccgcgcg gtgcgcaacg cgcgccgcgt
     1261 cgccttcgtg ctcaatctgt ccgagcagct cggcgagacc aaaggcatga gcgccgccgc
     1321 gcacatcgac gcgatgaccg ggcacgtgcg gcggctgcgt ctcgacgtgg tcctcgtgca
     1381 cgagggaccc tcaggcggga tgcgtcgtcc cgtgcatctc gacgagcagt ccattcgcgt
     1441 tccgatcgtg aaggccgatc tggtcagcga cggcgtccac gatccgctca agctcgccgc
     1501 gaccttcaag acgctgctgt gaagtcccca cgggcggcgt cgttctcgcg ccgcgtcaaa
     1561 gaagaactgg cgcacatccg cgcgccgcgc gaatgctgcc gccgcagcga gatggtcggc
     1621 atggtccgcg gcgggggcac gctccacatt ctgcccggcg gccgctatgc gctcgaggtc
     1681 gaggcgcctg acgcggccgt ggcccgggcc gtctattccg cgctgtcggt cctcggcggc
     1741 ttgcccgagg tgcggcagta ctcgccggga cgcgcgcatc cgcacgagcg ctacgtcgtg
     1801 cggatcgagt cgggagaggc gagcctgttc tcgcgcgcgg gagcgctcgc cgcggacggc
     1861 ctgcccgcga ccggcgtgcc gcgcggcgtc ttcgagcggc gctgctgcgc cggcgcgtac
     1921 ctgcgcgcgg cgttcatcgt ccacggcacc gtctcgccgc cccgcagcgc gacgcacctg
     1981 gagatccggg ctcccgacgc cggcaccgcc gaaggcttga ccgagctcgg tcacatggtc
     2041 ggcgcgcggc tgcgggtgcg cgaccatcgc ggccacgccg tctacaccaa ggacgtctcc
     2101 agcgtaggcg cgctgctcgc tgcgatgggc gctcatcaag gctgcctcga atgggaggcc
     2161 ggcggtgtct gggcgacggt gcgcaacgag gccaaccggc tcgccaactg tgaccaggcg
     2221 aacgtccggc gcacggtgag ggccagcctg cagcagcgcg acgcgatccg ccgcctggat
     2281 gccgagggcc ggctggacgg gcttccttcc ggccttcggg acgctgcccg ggttcggctc
     2341 gagcatcccg acgccagcct cgacgagctc gccaaagagc ttcgcatctc caaaagcgcg
     2401 atggcggacc ggctgcggcg cctgacggct ctggcgggat aagggtggat tcgagccccg
     2461 ccgggcacgg cactagactg agagaaaacg aacgctcaca cgggaggacc cagtggccat
     2521 caaggtcggc atcaacggct tcggccggat cggacgcaac ttctatcgcg cggtcgccaa
     2581 gcagggcgcg gacatcgaga tcgtcggcgt gaacgacatc accgatcccg cgacgctggc
     2641 gcacctgctg aagtacgact cggtgcacgg ccgtctcgac gccgaggtca agccgaccgg
     2701 tgacggcatc agcgtcagcg gcaaggagtt ccgcgtcacc gccgagcgcg acccggcgaa
     2761 ccttccttgg aaggagctcg gcgccgagat cgtcatcgag tcaaccggcc tgttcaccga
     2821 ccgcgacaag gccgccaagc acatggacgc cggcgcgaag aaggtcatca tctcggcccc
     2881 ggccaagggc gaggacggca cgttcgtcat cggcgtcaac gcgcacgagt acgacccggc
     2941 caagcaccac atcatctcca acgcctcctg cacgaccaac tgtgtcgtgc cgatggccaa
     3001 ggtcctgcgg gatgcgttcg gcatcgagaa gggcctgatg acgaccgtgc acgcctacac
     3061 caacgaccag aacctgctcg acctcgcgca caaggacctg cgccgcgccc gggcgagcgc
     3121 cgtcaacatc gtgccctcgt cgaccggcgc cgcgaaggcc gcgagcctgg cgatccccga
     3181 gctcaagggc aagctcgacg gcttcgcgat gcgcgtgccg gtgccggacg gcagcgtcac
     3241 cgatctcgtc gtgatcctcg agcaagaggt caccgccgcc gacatcaacg ccgcgttcca
     3301 ggcggccgcg tcgtcgggcc cgctcgcggg cgtgctcgag tacaccgagg acccgatcgt
     3361 cagcagcgac atcgtcggca acccgcactc gtgcatcttc gactcgaagc tcacgatggc
     3421 caacggctcg ctcgtcaagg tcgtcggctg gtacgacaac gagtggggct actcgaaccg
     3481 gctcgtcgat ctggtccagc tcgtcgcctc gaagtagccg tgaagctgcg cacgatcgac
     3541 gacctggacg tcgccggtaa gcgcgtgctg gttcgtgcgg acttcaacgt tccgctgaac
     3601 ggcggcgcca tcgccgacga cttccggatc cgcgccgcgc tcccgacgct catcgagctt
     3661 cgcgagcgcg gtgccgcgca gctcgtgctg tgctcgcacc tcggccgtcc caagggagcc
     3721 ccggatccga agtactcgct cgcgccggtc gcggcgcgtc tgtccgagct gctcggagcc
     3781 ggcgtggcgc tcgcgccgaa cccggacggg ccggtcccgg cgggcgcgcc ggtggtgctg
     3841 ctcgagaacc tgcgcttcca tccgggcgag accaccaacg acgcggcgtt cgccaaagcg
     3901 ctcgcggcga tggccgacgt ctacgtcaac gacgcgttcg gtgcggcgca ccgcgcgcac
     3961 gcgtcggtcg cgcacgtcgc gaccgagctt cccgccgccg ccggccgtct gctcgagaag
     4021 gagatcgcgg tcctgagcgg actgctggag agccccgagc ggacgttcgt cgcggtgctc
     4081 ggcggcgcga aggtcagcga caagctcaag gtgatcgaga acctgctcgg cgtggtggac
     4141 cggctgctga tcggcggcgc gatgtgcttc acgttctacg ccgcgcaggg gctcgagatc
     4201 ggcaagagcc tgttcgagcc cgatcaggtc gagaccgtga agcggctgat ggccgaggcc
     4261 ggcgacaagc tgatgatccc cgccgacgtc gtcgtggcgc gcgagccgaa agcgggcgtc
     4321 accgccaccg aggtcaaggc ggatgccatc cccgccgacc aggccggcta cgacatcggc
     4381 ccggaggcgt gccgcgcgta cgtggacgcg atccgcggcg ccgcaacggt cttttggaac
     4441 ggcccgatgg gcatcttcga ggttccgccg ttcgacaagg gaacgcgcgc cgtggccgcg
     4501 gccatcgcca agggcggcgc gtactcggtc gtgggcggcg gggactcggc cctcgcactg
     4561 gaccgcttcg gctacgccga cgggatcgat cacgtcagca ccggcggggg agcgagcctg
     4621 gagctgctgg aaggccgcga tctgccgggg ctcgtgccgc tgtgggtccg atagcccggc
     4681 ttcgccggga ggaaacgggg aatccggcag cgaaccttcc gcccacaccg gccacgagcg
     4741 ccggggcacg agggggagac gccatgcccg ttcgccgaag catccttgcc gttctgctcg
     4801 tcctcggggt cgccccggcg tggtcctcgc ccggcgaggt cgacatccgg gcctcgacga
     4861 gcgaagcagg cagcgggtcc gtcggggaat gcggcggctt cgcgccttcg tcaccgtcgt
     4921 gctcgttcac cgggaacctg cccgcggcga gcaccgtgca cctgtcgggc cagctggagt
     4981 tcaccggccg gatccggatc gtgctcaaga catcgaccgg cgagtacatg gcgtcgtgcg
     5041 acttcctcgg ggtcttcgac ccggtctgcc ggtcgaacac ctccggcgtc ctgcagatcg
     5101 gccagtcctt cacgttgacc gcgaccgcat cggcgtacaa cccgcacgac cgaagggatc
     5161 tcggcgacgc cgccgtgggc cggtggcgcg tctccatgac cgcgaactga tcccgcgccc
     5221 tcgtccgccc ggctcgccgc tcgattcgcg ggcggatatg aaggcccgcc ggggcctcga
     5281 ataggatgcg cgcactatga cgcgcaaacc gctcctcgcc gggaactgga agatgcacaa
     5341 gacgcacctc gaggcgatcg cgatcgtcca gaaggtgcac tacctgcttc gcccgcaaga
     5401 tctcgatgcc gccgacgccc tggtctgtcc gccgttcacc gcgctgcgcg ccgtgcagac
     5461 gatcatcgag tccgacgaca tgcgaatcgc tctcggcggt cagaactgcc actgggagaa
     5521 ggaaggggcc ttcaccggcg aggtcagcgc gccgatgctc gcgcggctga agtgctccta
     5581 cgtgatcgcg ggtcatagcg aacgccgcgc gtacttcggc gagagcgacg agatcgtcaa
     5641 caagaaggcc aaagcgatct tcgccgccgg gatgaccccg atcgtgtgcg tgggcgagca
     5701 gctcgacgac cgcgaggccg gccgcgcgca ggacgtcgtc ggcgcgcagc tgaaggggag
     5761 cctcaaaggc ctcggcgccg agcagctcgg cgcgatggtg atcgcctacg agccggtctg
     5821 ggcgatcggc accggcaaga acgctacgcc ccaggacgcg caagacatgt gcgcgttcat
     5881 ccgcggcgag gtcgcaaagc tgggcgggca gccggccgcc ggcaccgtgc gcatccagta
     5941 cggcggaagc gtgaaggccg gcaacatcgc ctcgatcatg gaaggccccg acgtcgacgg
     6001 cgcgctcgtc ggcggcgcga gcctggatcc cgaggagttc gcattgatcg tgaggtatca
     6061 gggatgaaga aggttctcta cgtcgtgctc gacggcctgg gcgacgatcc gctgcccgag
     6121 ctggacaacc gaacgccgct cgaggccgcg gaaaccccgt ggatggaccg gctcgcgcgc
     6181 gacggcgcga acgggctcgt gtatacggtc ggcaagggca tcgcgccgga gtccgacatc
     6241 gcggtgttcg ccgtgctcgg ctacgacccg cgcgagcatc attcgggccg cggccccatg
     6301 gaggcgctcg gaagcggcat ggacgttcgc gacggcgacc tcgtctaccg catcaacttc
     6361 gcgacggtcg agccgggcga cggccccaac tggacgatcc tggatcgtcg cgtcggccgc
     6421 gacctgacca gcgaggaggc gcacgcgctg gccgccgagg tcaacgcgaa cgtgaagctt
     6481 ccgggcgcga ccgccgagct gctggccacc gtcgagcacc gcggggccct gctgatccgg
     6541 cgggaaggta aggcgctcag cgcggagtcc acgaacaccg atcccgccta cggacgcgaa
     6601 ggagcgctcg gcgtcgcgaa gctgaccttc gacaatcacg tccaggcgtg cgagccgctg
     6661 cccggccacg agggcgacga ggccgcggtc gccggcgccg acctgaccaa cgagttcctg
     6721 cggcaggcgc acgccatcct cgaggccagc gaggtcaacg ccaagcgccg caatgcgggc
     6781 aagctcgccg gcaacatcat cctgacccgg gacggcggag accatctgcc gcacccgccg
     6841 cccttcaagg agcgctacgg cgcgcagtgg ggttgcttcg tcgagatgcc ggtggagcgt
     6901 ggcatcagca tggtgctcgg catggggcag gtcgaggtcc cgatgaaagg gagcggcacg
     6961 cacggcgagc agtacgcgac gtgggccgag accgcgctcg aggccatcca ggagtacgac
     7021 gggctctaca ttcacatcaa gggtccggac gtgcccgccc acgacggcag gggccaggac
     7081 aagcgagacg tcatcgccga gatcgacgaa tcctttttcg ccaccatcgt gccggagctg
     7141 ggcaaggacg tgatcgtggc ggtgaccgcc gatcacgcga cctcgtgcgc caagaagtcc
     7201 cacaccgatg atccggtgcc gctgctggtc tgggggccgg gcgtatccga ggacgccgtg
     7261 cagaccttca gcgagaccgc ggccgcgaag ggatcgctcg gtttgcttca gggcgtcgac
     7321 atcatgcccc ggctgatgga gcttgctcgc gagtaggaat ttcctggcag gcgctgccag
     7381 attttggcta atttcgggcc ggtgcaggat gaggcaaccc tttttgcggt agggctcgta
     7441 gtacatcggg aacgcctgcg accccatcgc agggggccgc gcttgtttgg agcagggaaa
     7501 ctcgtcggat atagtcccgc cgtctacgga gcggggaggg agagttgcgt atgcaaaaga
     7561 aacgcatgtt ccagcttttt gccggagccc tcgtgctgtc gctggttgcg gcagcatgtg
     7621 gaggcgacgg gaacggtggt ggcagcggca gcggtgacgg caaggccggc ggctcggcca
     7681 tcgcggagct cggcgagccc gacggcttgg cgccgcacct gtgcggcacg acgtcgtgct
     7741 ccgagccggc gaaccggatc ttcgattcgc tgctcgagta cgactggaag acggccaagg
     7801 tggtcaccac cggcgcggcg acctcctacg aggtgagccc cgacggaacg aagatcacct
     7861 accagctgcg caagggtgcg acgttccaca acggcgagcc ggtcgacgcg gcttccttca
     7921 tccgttcgct cacgctgacg gcatcgaagg acgcggcttc cgacgtcgcg tatcacctcg
     7981 gcggcatcaa gggattcaag gacctcaacg aaggtacggc caaggacttc ccgggcgtca
     8041 agcaggggaa ggacgagtac gagctgatcc tcgagctcga ccagccgaac gccgagttct
     8101 tcgtccgcac gggccacatg gtgttcgcgc cgatcccgaa ggtcgcgctc gccgccgacg
     8161 gcacgttcaa caaggactac aacgaggcgc cgatcggcaa cggcccgtac atggttgacg
     8221 gcacctggca gcacaacgtc agcctgaagc tcaagaagta cgccgattac aacggcccgg
     8281 tgaagggttt cctcgacacg atcgagttca agatcttcga gaagctcgag accgccttcc
     8341 tggagttcca gggtggcacg ctcgacacca cgccggtcac accggaccag ttcgacgccg
     8401 ccgaggcgca gtacggcgac gcgttcatcg agcaggccac ggccgttctg acctacctgc
     8461 tcgcgaacaa cgcgaaggcc ccgacgaaca acgccgattt ccgtcgcgcg ctgtcgctgg
     8521 ccatcgaccg cgaggagatc gtcaaggccg tcttctcgaa ccgtcgcatc ccggcgaaga
     8581 gcatcgtccc gccggcgtcg cccggtcacc gtccggacgc gtgcaagtac tgcacgttcg
     8641 acctggacaa ggccaaggag ttcctccaga aggcaggcgg tccgaagaac atcaacctgt
     8701 ccttcaacag cggtgcgggc cacgaagagt ggacggcggc cgttgcgtcg cagctcgaga
     8761 agaacctcgg cgtcaaggcg cagctgatcg gcaagacgcc gttcggcgac tacatcaagt
     8821 acctggacac cgacgagtgg ggcggcggcc tgggtcgtct cggctgggcg caggactacc
     8881 ccacgccgga caactggctg ttcccgctgc tgcactcgca gtcgggcgac aaccactcca
     8941 agtacaagaa ccccgagttc gacaagacga tcgaagagtc ccagcggacg ctcgacgcga
     9001 agcagcgtct ggcgctccag cagaaggccg aagacatcgc tctcgaggac atgggcttca
     9061 tcccgatgtt ctacggaaag tcggcgcgcg tgttcaacaa ggacaagttc gcgtcgttcc
     9121 cgttggagct ccagtccggt aacccggcct gggaggaagt gtccatcaag tagcgaataa
     9181 gagagcacga atcggagcgg ggacttgccg ctcgggcaag tccccgctca cggttcgcag
     9241 gagaacagca cgtgaatcgc tacatcgccc gccgcttgct tcagttcatc ccggtcttgc
     9301 tcgggacgct gacgctattg ttcgtagcgc ttttcatcct tccgggcgat ccggtacagg
     9361 cgctggtggg cgagcgcccc gtctctgaag gcttccgtca ggccatcatc gacaagtacg
     9421 gcctcgacga tccgcttcac gtccagtacg gccgctatct caagcgcctc gtgcagctgg
     9481 acttcggcga atccgccgcg agccgccgcg acgtgtggga catcatcaag gagcgcgtcc
     9541 ccgtcaccgc acgccttgcg gtcgccgcaa cgttctatct gttgatcctc gggatcgggt
     9601 cgggcgtgat atccgccgtc cgaagatacg gcatcagcga tgccgccgtg accgtcggaa
     9661 ccatcgtcct ggtgtcgatt ccggtgttcg tgctcggact gctgctgcag atcttcgcgc
     9721 tgaagacgaa agacgttctg ggattaccga tctcagggct gaatgccggg ctgaagtcat
     9781 acatccttcc gggattcgcg ctggcgtcgg tctcctccgc gtacatctcg cgattgcagc
     9841 gaaccgcgct gctggagacg ctcggaaccg actacgtgcg aaccgctcgc tcgaagggcc
     9901 tgcgggagcg ccgcgtcgtg atgaaccacg cctggcgcaa cgccctgatc cccgtcgtga
     9961 cctatctggg tctcgacatc ggcacgctgc tgaccggcgc cgtgctcacc gagacggtct
    10021 tcaacatccc gggcctcggc cgcgcgctcg cccaaggggt gacgcagcag gacaaccagg
    10081 tcgtgctcgg cgtctcggtc ttcatcgtgc tcgtgtatct tgttgccaac ctgatcgtgg
    10141 acatcctgta cgcggtcctg gacccacgaa tccgctacca gtagcgattc ttcgagtgtg
    10201 acgacgtgac gacgaccaag accgatcccg accagcctcg cgagccgctg tcctcggacg
    10261 tgcccgaccc gacggccgtc ccggacggcc tgctcgaggt caccagcttg cgcggcgagt
    10321 tctggcgccg gctgcgcaag aacaagcttg ccgtcgccgg gatgatcatc ctcgggttga
    10381 tcatcttcgc ggcggtcttc gccaacgtca tcgcccgcca gcccacggcc gggttcttcc
    10441 cgtcagaggt tcgtgagggg ccgagctggc ggcactggtt cggcaccgat ctgctcggcc
    10501 gcgacatctt cgcgcgcacc gtccacggcg cacgcttctc gctgatcgtc ggagccagcg
    10561 tggtgtccgt cagcctcttg atcggcctga cgatcggcgg gctcgccggc tacttcggcg
    10621 gctggatcga cacgctcctg tcgcgcctga tcgacgtgtg gctcgggttc ccgtacctgg
    10681 tcggcgcgct gatcatcgtc gtatcgctcg gcggaggaaa gttcgcgctc gtcgcggcgc
    10741 tctcgacctt cggatgggtc acgatcgctc gtctgttccg cggcagcgtg atcgcgatca
    10801 gggattccga gtacgtcgag gccgctcgcg ccctcggcgc cggaaacggc cgcatcctgc
    10861 ttcgccacgt gctgccgaac gccgtcacgc cgacgctcgt ctacgccttc gcgctcgtgg
    10921 gaaccacgat catctccgag gccgcgctgt cgttcctcgg gttcggtgtg caggagccgg
    10981 agacgtcctg gggcctgatg atcgcgcgct cccagtcgca gatgctcgcc ggccacgcgc
    11041 acatggttct gttcccgtcc ctcgcgctga cgatgaccgt gctcggtttc atcctgctag
    11101 gtgacggttt gcgcgactcg ctcgacccga ggctgcgata gccgtgtccg acgtccgcgt
    11161 cgaaacgact ccgaccaccc cgcccggcga gcacttgctc gaggtcgagg acctcgccgt
    11221 gtccttcttc accgacacgg gcgtggtcac cgccgtggac ggtgtgtcgt tctcgctcga
    11281 cgccggccgc acgctggcga tcgttggcga atcgggctcg ggcaagaccg tcagcacgca
    11341 ggcgatgatg ggtctgatcc ccaagccgcc cggaaagatc atgcgcggta ccgcgaagtt
    11401 ccgcggccgc gacctgctga cgatctccga cgaggagatg cgcaagattc gcggacgcga
    11461 gatcgcgatg atcttccagg agccgctgac cgcgctcaac cccgtccacc gggtcggaag
    11521 tcagatcgtc gagatgatcc gctcccacga agaggtctcc aagaaggaag cgcggcagcg
    11581 tgcgatcgag ctgctcaaga ccgtcggcat cccgcagccg gacaagcgcg ttgacaacta
    11641 cccgcacgag ttctcgggcg gcatgcgcca gcgcgcgatg atcgcgatgg ccctcgcgct
    11701 gaacccggcc atcctgatcg ccgacgagcc cacgaccgcg ctggacgtca ccgtgcaggc
    11761 gcagatcctc gagcttctga aggggttgca ggaggagttc gggatgggcc tgattatgat
    11821 cacccacgac ctgggtgtgg tcgcgcagat ctcggacgag atcctggtga tgtacgcggg
    11881 ccgcatggtc gagcgcggcg atcccgaaga cgtgttctac aagccgcagc atccctacac
    11941 ctggggtctg ctgggaagca ttccgcgcgt gctggaagag gagttgaccg acgagcgtct
    12001 ggtcaacatc cccggtatgc cgccgtcgct gcagcgagtg ccgccgggat gcccgttcca
    12061 tccgcgatgc accttccggt tcgagccttg cgacacggag cgccccgagc tgctccagcg
    12121 tggtcaccac gaggacgcgt gcttcctcga gcaagacgag aaggaagccg gccgcgccga
    12181 tatcctccat ctggggacca agcccacgcc caagccgaag tccaagccgc cgagcgtggc
    12241 caggaaggca gggacccggt cgtgaccgat atcgccacca cgccgcaggc cgcgccgtct
    12301 cgctcgggca cgccgctcgt cgaggttcgc aacctcaaga agtacttccc gatcctcgaa
    12361 ggcatcgtgt tcaaaaagga ggtcggccgc gtccacgccg tcgacgacgt ctccttcgac
    12421 atctacgaag gcgagacgct cggcctggtc ggcgaaaccg gctgcggcaa gaccacgacg
    12481 ggccggctga tcctgcgttt gctcgagccg acgtcgggca cgatcaagtt cgagggcgag
    12541 gacatctccg agttcgacac caatgcgctg cgcccgctgc gccgcaagat gcagatcatc
    12601 ttccaggacc cgttcgccag ccttgacccg cgcatgacgg tcggctcgat catcggtgag
    12661 ccgctcgaca tccaccggct cgccgacggc gaccagaagc tgcgccgcac ccaggagctc
    12721 atggagatgg tcggcctcaa ccccgaccgc cacaaccgct atccgcacga gttctccggc
    12781 gggatgcgcc agcgcatcgg cgtcgcgcgc gcgctcgccg tcgagccgaa gctgatcgtg
    12841 tgcgacgagc ccgtctcggc gctcgacgtg tcgatccagg cacagatcct caacctgctc
    12901 gacgaccttc agaaagagct cggcctgacg tatctgttca tcgcccacga tctgtcggtc
    12961 gtgcgtcacg tcagcgaccg cgtcgcggtg atgtacctcg gaaagatcgt cgagatcgcc
    13021 accggccgcg agctctacac cgacacgcgt cacccctaca gcggcgcgct gctgtcggcg
    13081 atccccaagg ccgacccggt cgtggcgcga agcacgaagc ggatcttgct gaccggcgac
    13141 gtgccgtccc cggtgaaccc gccgagcggc tgccggttcc atccgcgctg tccggtcgcc
    13201 aacgagcgtc ccgaagagcg gctgccggtc tgctcgacgc aggagccgcc gctcgcctcg
    13261 atggacggcg atcacgtggc cgcgtgccac ttcccgctgc cgccgaagac cggcgcggac
    13321 ctgagcggga tgaatctcgc cggcggagcg ccccgcgacg acgaggtgcc ggtgaagccg
    13381 aagacggccc gcaagacctc gccccgcaag accaccggca agacgaccgc gcgcaaggcc
    13441 gcgtccggcg cgcgaaccgc ctcgcgcgcc ggcaaaaaga ctgcggcgaa gcctgccggc
    13501 aagaccactt cgtcacgcgc ccgaaagtcg ccgccgggcg cccggtagcg gtgaccccgg
    13561 tggcgttcac gacggcgcac cccaggcgcg gtctccgagt cctcgcgctc gcgtgtgtgc
    13621 tcgcgctcgc cgccggggca tgcacgcaag gggccgacgg cgagtcgcgg ccgggcgaga
    13681 cgcccgagcc cggcggcacg ctgcgcatcg ccgccggggg cgacgtggac tcgctggatc
    13741 ccggccgcac ctacaccacg ttcggcttca tgctctttcg cgtgatggtt cgaacgctcg
    13801 tctcctacgc ccccaagccg ggtgaggccg gcaacaagct cgtgccggat ctcgcggagg
    13861 acacgcccac ggtctccccg gacgggctca cctacacgtt cgtgctgcgg ccgggcgtgc
    13921 gctaccagcc cgagatcgcc gcgggccgcg aggtccgctc cgaggacttc cgctacgcga
    13981 tcgagcgcgg cttctacgca tcggtgtcca acccgtacgc cgacctgtac ttcggcgacg
    14041 tctttgccgg cgacgagaag ttcatcgcgg cgccgagcca caaaacccat atcgacggta
    14101 tcgacgtcag cgatccccgc aagctggtac tgcgcctgaa gcggccggtg ggggatctgc
    14161 tctggcggct cgcgttgccg ctcgcggctc cggtcccgcc cgagtacgcg atcccgctcg
    14221 acggcggcac cgagtcctcc tacggcgacg ccttcgcctc gagcgggccg tatcagatcg
    14281 aacgtcagaa cgcgacggtc accggctacg tgccgggcaa ggcgatccgc atggtgcgca
    14341 accccaactg ggacgcccgg accgatccga ttcgcaaggc gtacccggac cggatcgagg
    14401 tcaccggcgg ctttgccgac ctcgcggccg cgaccggcaa gatccttgcg ggcgagatgg
    14461 actacaacgg ggacttcccg atcccgcccg aacagcagaa ggacatcctt tcgagcgcgt
    14521 ccacgaagga tctcattcac tacaacgcga ccagctgcat gcgctacgtg tcgatgaacg
    14581 ccacgattcc gccgttcgac aatccgctcg tacgcaaggc cgtgaactac gccctggaca
    14641 agcgcggcat gcgcgcggtc cgcggcggcg agcgcaccgg cagcatcgcg acccacgtgc
    14701 tgctgcccgg gcttccgggc ttcaaggagg tcgggcagaa ggactacgac ccgtacagca
    14761 gccgcgattt cgcgggcgac gtcgagaagg cccgcgacct gatgcgcgaa gccggctatc
    14821 cgaccggtct gtacacggga ccgtcggtcg tcatggccgg cgaggcaagc gagatcgagc
    14881 ggcgtaccgg cgacgtgatc gtctcgtcgc tcgaaaagat cggcatcaag gtgcgccgcg
    14941 aagagcacaa acggccggcc ctgcttgcgc gcatgggcgt gcagaagtcg ggcatcgcca
    15001 tcggtgcgag cgccgcatgg tgctgggatt acccggatgc gtatacggtg atcgcgccgc
    15061 tgttcgacgg gcgtcgcatc cgggagaagg ggaataccaa ctactcgcag atcaactccc
    15121 cgtcgctaaa cgagctgatc gacaaagcga gcggcgcgtc cggggatgat cgcgcagccg
    15181 catgggccgc cgcggacaag ttcgtgatgg acttggctcc ggtggtgccg tggctatggg
    15241 attcctcgcc gcatctggtg tcgccgcgcg tcgtcggcta ccagttcgca ctatcctcgg
    15301 tctcgatgga tctagccgtc gcggccgtcc gtcaggacca ggaatgattc gaaggacaat
    15361 ggagggaacg tgatgaggac acgattcggg ggagcgcgcg cgggggccgc ggtgctcgtg
    15421 cttgcgctgg ttggcgccgc atgcggcgcc gatgcgccgg ccgaggaagg cccggcgcga
    15481 ggcggcacct tcaagatcgc cgcggcgggc gacatcgact cgttcgatcc cggcaagacc
    15541 accgggacga tgggctcgct gctgatgcgc gtgctcgtcc gcacgctcgt cgcgtacccg
    15601 gccgtggacg gcgagaccgg aatcgagccg gtcgccgaca tcgcctcgtc cgagccggcc
    15661 atctcgcccg acggcaagcg cgtcgtgatc tccctccgtc ccgacgtgaa gtaccagccc
    15721 gaggtcgccg gcggccgcgc ggtccagtcg cgcgacatcc gctacgcgat cgagcgcggc
    15781 ttctacccgt cggtcgccaa cccgtacgcg cggacctatt tcgccggcat cttcgtcggc
    15841 gacgaggcgt tcctcgcggc gccggggccg gacaagaaca tcgcgggcat cgacgtgtcc
    15901 gatcccgcgc gcatcgtgtt cgacctgaag gcgcccgtcg gcgatctgct caaccggctc
    15961 gcgctgccga tggccgcgcc cgtgcccgag gagtacgcga agcagttcga cgtcaagccg
    16021 gtctccgact acggcgcgaa cgtcgccgcc accggcccct atcagctcga gcggagcggc
    16081 agcaccgtca ccggccacaa gccgggcgag tcgatcacgc tcgtgcgcaa cgagagctgg
    16141 accgcgagca gcgacccgat ccgcaaggcg tatccggaca agatcgaggt caccgaaggg
    16201 ctcgacgaga tcacgcgcac ggtcgaccgg gtgctgtcgg gcgagttcga ctacgccgcc
    16261 gacatcggcc tgcccgcgga ccgcgccgcc gagatcctca acgacgacac tgccaagacg
    16321 cagctgttct tcaacgcggg caactgcatg cgtttcatct cgttcaacac caccgtcaag
    16381 ccgttcgatc agctgaaggt gcgccaggcc atcgcgaccc tgatcaacaa gggcgatctg
    16441 ctcgaggcgc gcggcggcaa gcggaccggc gacgtcgccg gtcacgtgct catgccgggc
    16501 atgcccggct tcctcgaggc cggcggccgt acctacgatc cgttcgcccg cgcggtcgag
    16561 ggcagcacgc tcgtgcgcgc gcaggagatg atgaaggagg ccggctacac gacgggccag
    16621 tacgaccccg agaagaagga gcggcccgtt ctggtggtgg gtatcgacga ggacttccac
    16681 cgccggatca ccgacatcgt cgtcaaggcg ctcaccgacc tcggcatccg cgtggaccgc
    16741 caggattaca aggcgagcga cgtctccggc cgcttcgcga gcgtgccgcg gtcgaacgtg
    16801 gccgtgctgc cgaacgcggc ctggtgctgg ctgtatccgg acgccgcgac cgtgatcccg
    16861 ccgctgttcg acggacgcaa gatcgcgccc gcgggcaaca ccaacttctc gctcctcaac
    16921 gaccccgagg tgaatgcgct catcgaccag gccctggccg ctcgcggcaa ggagcgcgcc
    16981 gacctgtggt cgcgtgccga ccggctgatc atggaccggg tgccggttct gccgtggcta
    17041 tgggagcgcg tacccacgct catctcgccg cgcgtggtga acttccagta tctgcttccc
    17101 accacgtcgc tggacctggc cgtcgtggcg gtgaagcagg ctcccgcggc gtgaccggtc
    17161 gcagggcgat cgcggccgcg tgcatctgcg cggccgcgtt cgctgcctgc accggctcgc
    17221 cgggccccgg tcccggcgac ggcgaccggc ccccgggcgc gacgcgaggc ggcacgctca
    17281 ctttcgcgct cgccgacgac gccggcgtgc tcgatcccca gctcgcgtcg cagccgggcg
    17341 cgttcgcgtt gatccgcgcg acccagcggg ggctgatggc gtttccctcg tccgaggacc
    17401 cggccgaggc tgccgtgccc gtgcccgacc tggccgccga cgagccggac gtctcggcgg
    17461 acgggcggac cgtgaccttc acgctccggc gcgggccgtc gttcggtccg cccgcgtcgc
    17521 gtccggttga ggcagccgac gtcaaggccg gcatcgagcg gctgttcgtc acccgctcgc
    17581 cgctcgcgac ctacttccgc gtgatcgtgg gcgccgccga cttcgaagcc cgccgcgcgc
    17641 cctcgatcgc cgggatcgag acgcccgacg cgcgcacgat ccggttcagc ctgaccgccc
    17701 cggccaacga cctgacctgg atgctcgccc acaccgccgc gagcgccgtg cccgccggaa
    17761 cgccggcggc ggtcggtccc aaggacctcg cgtcctccgg cccctatcgg gtggagcggg
    17821 tcgcggccgg acgcgagatc cgtctcgtcc gcaacgacgc ctgggccgcc ggctcggacc
    17881 cggtccgcgg ggcctacgtg gacgagatcg tggttcgggt cgggtccgcg gccaccgccg
    17941 acctgaccgg cgacgaagtt cccgtcccgg ccggggccga gggacgcggc gtggatgccg
    18001 gatgcctgct gtatctgttc gtccggccgc aggggccttt cgccgagcga aacgcccgag
    18061 ccgcgatctc gctggcgatc gaccgccgcg ccctggtcga ggaggtcgcc cgggccacgg
    18121 gcaccttctc ggcgacggcg acggtgtcgt tgctgccgcc gatgctggcc ggggcgaccg
    18181 aacggccttt gccgccgggc gacgcggccg aggcgcgccg cttgctggcg ggcgcctcgc
    18241 cccaagtggc gttcggccgc gaagcatccg ggccggtcgc cgcgcaggac aaggccgcgg
    18301 ccgacgtgat ctcgcgcatg ctggccgccg ccgggatccg caccgtcccg ctcgccgtgc
    18361 gctcgcccgg ctcgatctac gaggtctacg ggcggggcgc ggctccgatg ggcctggcgc
    18421 gctggtgccc ggactggccg ggacgcggcg cacgcacgat gatcgccgcg ctcgccggca
    18481 ccggcgcggt ggccaactac tcgggcacct ccgacgccac cctcgacgcg ctcgtcaccg
    18541 cggccgtcgc cgagaccgac ccgggtcgca tcgccgcgca gaccgccgcg gccgccgacc
    18601 gcatgctcgc cctcgtcacg atcgtgccgc ttgccttcct gagcgaccgg gtcgcggtct
    18661 cgccccgggt tcggggcttc gtccctcatc cgttcttcgt ccggggagac cccaccaacg
    18721 tctggctcga agagctcgct ccctcgcccg ccccgtcccc cacggcgccg gtcccggcag
    18781 ggggtgcatc gagcatcgcg gttcggtaca cttctcgggt tcccacgacg aatggaggag
    18841 tgtcttgacg cccgttatcc tcgggttcca gatcctgtcc gcctgcctgc tgatcctgtt
    18901 catcctgctg catgcgggtc gaggcggcgg tctgtcggac atgttcggcg gcggcatgca
    18961 cgcggccggc ggctctaccg tcgtcgagaa gaacctggac cggatcaccg tgatcacggc
    19021 gatcgtgttc acgatttcca cggttgcgct gcatttccgc atccggccgt aaactctcct
    19081 gcatgtccgt cctgcccggc cgcatgcggc gaacggctgc ggctgccctc gcgctcgccg
    19141 cgctgctcgt ctcctgcagc gaagacgctc cgtcccgcga accggagaac ccggtcgccg
    19201 gcggcacgct ccgcgtctcg gtccgcgagc tgaactccct ggatcccgcg aaggcgcgcg
    19261 ggcagggcgc ggcgctggtg ctggagcagg tcttcgattc gctcaccggc gtcgacgacg
    19321 cgggcaatgc cgtcccggcc gccgccgaga aatggaccac ctcacccgac ggcctcacct
    19381 ggaccttcac cctcggccgc tccgtcttcc acaacggcga tcccgtcacc gcctacgact
    19441 tcaagttcgc cttcgaccgg atcgccggaa agacgctcgc ctccgaggcg gccttccagc
    19501 tcgagccggt ccgcggattc aaggcggttc gcatcgacgg cacgacgcag gcgctgtcgg
    19561 gcgtcgaggt cgtcaacgcg tccacgcttc gcatcacgct cgatcggccg ttctacgagc
    19621 tgcccgtgta cctgtcgcac gccgcgctcg ggccggtctc gcagaagttg ctcaaccgcg
    19681 acccggcggc gttcgccaag catccgatcg gcaacggcgc cttcgaagtg gccgcgccga
    19741 tgagcgtcgc cggcgtgcgg ctcacccgat tcgacgggca cgcgggcgcg acggcctacc
    19801 tggatgcgct cgacgtcgtg atccacgaag gagccgacga cggctaccgc gcatatctgc
    19861 gtgacgaggt cgagatcgcc gaggttccga acagcgcgat cgagtcctcg cggggccgca
    19921 tcggaccgtc cgggttcacg cccctgtggg cggccgtcta ctacggaccg aacatgcggc
    19981 tcgacaagtt caagaatccg gagtttcgcc gggccatctc gctggcgatc aaccgcgcca
    20041 cgatcgcgag ctccgtctac ggcggaacca aaatgccagc gaccggcgtg atcccgctcg
    20101 gcatccccgg gttcccgccc gaccgctgcc gcgactgccg cttcaacccc ggttccgcgc
    20161 gcgatctgat caaggcggcg ttcccgtcgg gcccccccga ggtcgtgctc gaccatctgg
    20221 ccgcctcgcc ctcacgcgag gttgcggcgg cgatcgcggc gaacctgcgc gaggtcgggc
    20281 tgaccgtgag cctgcgcgcg cacgagtcgg gcgcctacct gaggctgctc gagtccggtc
    20341 agcaagagct cgccgagctc ggctggctcg ccgaggtgcc cagctccgac gggttcctcg
    20401 cccagcagct tcgctccggc tcgcccaaca atcaggtcgg gttctcggac tccgcgttcg
    20461 acaccgccat cgacaaggcg cgtgccacga aaggcatcgc cgagcggcgg gcgcaatatc
    20521 agtcggccga gacccgcgcg ctcgccgaga tggcgctgat cccggtggtg ttcttccgca
    20581 accatctcgc ggtcgcgcgc agcgtgcgcg gattcgacct caacggcgcg ggcctgttcg
    20641 acgcgtcgcg cgtgtggctc tcgaccgagc cgtgaccgcg gccgcgcggc ggcgcatccg
    20701 cgttgccgga gcgctcgcgg ccgccgcact gctcctgacc gcctgccccg ccggcgagcc
    20761 gaagcggccg gcgggcagtc ccaccgccac gccgctgccc accacgccgc cggaaggcga
    20821 gatccgtctc gcctggcccc acgagcccgc gaccctgaac ccgttcgtgc gcggcggtga
    20881 gtcgccgagc gctcgcgcgc tcctgcggcc gctgtggccg ccgctgttcg cggccggtcc
    20941 cggcggcgag gccacgccct ggctcgtcga tcgcgtcctg tccgaggagc cgctgtcggt
    21001 gcgcgtggcg ctgcgcgccg acgccgtctg gtccgacggc gagccgatca aggcatccga
    21061 cgtggtcttc acctggaaga cgatcgtcga tccggcgtcg aaggcgatcg cgcccgccgg
    21121 ctacgacgcg atcgcgtcgg tcacggccga gtcgcagacc atcgtcgcga tcaccttcaa
    21181 gcacgcggtg cccgcctgga ccgacctgtt ctcggccggc ctgggcgtgc tgcccgagca
    21241 cgcgctggcc ggaaagggca tcggcgagat cggggcatgg cccgtgtcgg gcggaccgtt
    21301 cgttctcgag cgctacacgc gagggctgga gatggttctt cgggcgacgc cccagccctg
    21361 gcgtgttcgc gcgcgcgtgc gcaccatccg ggtgatgttc gtgccggact ccacgaccgc
    21421 gatccagttg ctgcgagccg ggcgcgtgga cgtgctcggg ccgtactcct cgcccgactt
    21481 ttcccgccgg ctcacggccg aggacggcgt cgccgtctcg accgacaccg gcgccgtctg
    21541 gaccgggctc gtgctcaaca cggcatcgtc gatcctgtcc gatgcgcgtg tgcggcaagc
    21601 ggccgcgttc gccgtcgacc gtgccgcgat cgtcgaaggg ctcgttcgcg gtgaaggggg
    21661 cgggctcggc cggccgttcg tgtatccgga ggccggcagc gaaggcgtgc cctacgatcc
    21721 gcagcgcgcc gcgcagctgc tggccgaggc cggcttcgcc gagcggcggg gcggaacgcg
    21781 cgcgaaaggc ggcaccccgc tcgagatctc gatcgcgctc agcgggggcg acgatctggc
    21841 gcagcgcgtc gtgcgcgccg tgttcgcgca cctgaacgcg gccgggttcg ccgtgaacct
    21901 ggtctcgctc gaggacgagg cgctgtggcg cgactggctg ccgtcgtccc ggttccaggc
    21961 ggccctggtg accttcgccg atccgcccgg cggcgcgctg cgcgaccgct accactccgc
    22021 cgaggtcatg cccggcgggc tcaacgtgcc gcgagtcggg gatccggcgt tggatgccgg
    22081 cattgaagcc ggcgacgccg cccgcgtcgc cgccgagctc gagcggctcg ctccggtcgt
    22141 gccgctgtgg cgagcgagcg tcccggccgc ggcccggggc ggcgtccagg gcgtccgggc
    22201 ggtcgcctcg gccgacggca tgctcgtcct cgcccacgat tgggcgatct gagcgctccg
    22261 cccgagagcc ggggacggcc ccggccgcga gtgctaccat gcccgtctgc tgcacctatg
    22321 cgggtgtgct ggaataggca gacaggcatg gttgagggcc atgtgtccgc aaggacgtgg
    22381 aggttcaagt cctctcaccc gcactcggtt tgattggggg ctcttgccac tcgcttcgct
    22441 cgtggcctcc gcccccaaac ccccgcgaga aagagccccg gagccgaagg atttcatgac
    22501 gaatatgatg ggcatgctcc cgcgcaacgc cgtatgagca tggggatctg accgagaggg
    22561 cgagtactcc ggcgaccggg ccttactcgg caatgggccg cgaggagccg agtcacttcc
    22621 gaacgagcgt ttgaaagctg ctctttgaga agcactcttc cgatcagcgt gaatagaagc
    22681 cccatccgaa cccgccggct tttcattgct tcgatgtggg aacgagagga gcatgacggt
    22741 gcggagagcg tttctggtgg gcattcctgt cgggctggcc ggggtgatcg cgctcttggc
    22801 gatcgaggtg cgcctggcac ggcgggccga acgcatcgag ccgttcgatc gcagcgtgct
    22861 cgacggctcg atcgggggcg agagcgcgtc gccgctgcgc atcgcgtggc tcggcgactc
    22921 gaccggggcc ggggtcggtg cgtcctctcc tgacaatgcg ctgcctcgcg tcgtcgcgcg
    22981 ggggctgaga cggcccgtcg atctcaccgt gctcgccgaa tcgggtgcgc gcgttcgcga
    23041 cgtgctcgac gatcagatcc cgctgctcca gtcgctgcgg ccggattggg tcatcgtcgg
    23101 gatcggcggg aacgacgtca cccacctcac ctcgcgggcc tcgtttcgaa gcgacctcgc
    23161 ggatgtcctg cggcggagcg cggcagtggg tgccgagcga gtcgtgctcg tcgggatcgg
    23221 agagttcgcg gcgacaccgc ttttggcaca gccgctccgt gcgatcgccg gcatgcgtgc
    23281 cgaccggctc gccgccgatc agaagcgcgt cgcgcgtgag cgcggcgcgc tgttcgtcga
    23341 cgtcatcgag gcgacgggcg cggccttcgt gcgggacccg aagcgctacc acgcccacga
    23401 cgggttccat ccgagcgacg acggctacgc gctgtgggcg gatgccgtgc tgtccacgat
    23461 agaagcgaca cgtggctgat cacgcttcag ttcgcgctgg cctctcagga cgaggaaact
    23521 aggcgaatgt cgatctcgag agggctgatc acatatcggt cgcgcccggc ttttcctgat
    23581 tcggcaggga ggcgacccct cggcggagaa cagtaagcgc aagccgtcca gaaagggttt
    23641 gtcgatgaag cttcgcaccg ttgccgccgc gctcgcgctc gccgtccttc ccgcgtccgg
    23701 gctcgcgccc gccgccgccg cgaccgattg caccgatccg tcggccacga tgaccgcgct
    23761 gccggcagcc gccggcgcgg gtaccggagg ctacgtccgc cccgcggccg ctccgaccgc
    23821 gctggtcgtg ttcgctcacg ggtacacgca cgagtcggcg agctgggagc agcacatgca
    23881 agacgcggct gcccacggcg cgcttgccgt cacgcccgac tactcgtact cgatattcaa
    23941 gcgtggctgg cacgtctcgg ccggcgccca ggacatgatc gcggccgcga attacttcct
    24001 ggacttgtgc ggcgccacga tccgcgagac gatcctgttc ggcgtgtcga tgggcgcgaa
    24061 cacgagcggg ctggcgctgg ccgagaagcc gatgcgcggg ggcgcgccgc tgttcgacgt
    24121 ctggttcgac gtcgaaggcg ccgtcaacgt gatcgagacc tatgcgggcg cgagcgcgct
    24181 cgccggcgtg aacgccttcg ccgccaacgc caaggccgac atcgaggccg agaccggcgg
    24241 aaccttctcg caggtcccgg aagagtacgc gaagcgcgcc gtggtgaacc gcgtcggcga
    24301 catccgcgac tccggcgtca agggcgtcgt gctcgtccac ggcgtcgagg acgggctcgt
    24361 gccctacaac cagtcgcgcg agatcgtcgc gctgctgcgc gcccatggcg tgcccgcgga
    24421 catgaccacg gtggtacgca agggcagcgg cgagaacggc accacgctca gcggctacgc
    24481 ggcgcagggc agcccgttcg ccggccacgg cagcgaatcg agcgcgaccc agcgggtcat
    24541 ccgcatcgcc cacgaccgtc tgcacgcgat gctcgacgag atcgccgcga acaacgagcc
    24601 cgccgtcgag gaccgcgagc gcatcgtcga cgacgccgac acgatcgagt agctacagcg
    24661 acgctgcgtc ggacagcgcg gccccggaca ttcccgggcc ggttcgcgcg cccggcgcgc
    24721 cgtcgggcag catcacgcgg aacgacgcgc cgccgtccgc ccggtcctcg acccatgcgc
    24781 ggccgccgtg aagttcggac aggcgcgcca ccagcgccag gccgatgccg gtgcccggcg
    24841 cgtgctcgac gcgctgcggc ccttgacgga agacctggaa aatcgcggcc ttatggtcgt
    24901 cggggattcc ctcgccacgg tcgtccacga tgatcagcgt gccgccgttc atgcggcagg
    24961 cccggaccgt gatcgggagg ccggacggcg tgtacttcac ggcgttgcgc accagattcg
    25021 acacgatccg ttcgaccttg ggtgcgtcca cggcggcggt taggccgccg ggcacgtcca
    25081 cctcgatggg atggtcgccg acggcgacct catcgagcac gcgctgaacg acgtcggcca
    25141 cccgggtcgg tccgcggtgc aactgcgcga cgccgttggt gtacgcgttc aggtccagca
    25201 ggtccatcag aattcgctcc agacgctcgc cgctggccac gatccgatgc aaaatctccg
    25261 ccttctcctc gacggtcagg acgtggtcgc gggacatcag cgtcttggcg aagcccagca
    25321 ccgctgtcag cggcgtgcgg acctcgtgcg atatggccga gatgaacgtc gtcttcatct
    25381 ggtccacctc acgaagcgcc tcgaccgtct gccgctcgcg cgagagcatc tctgcatgct
    25441 ccgaggccag tcgctcgaac agcactcgct cctcgcgggt ggtgcgttcc tggcgggcca
    25501 ccaagccggc cgctccggcc gcgatcacga tgtagccggg acgaaggatc gcggcggccc
    25561 cgggcaggcc gccgtccatg gtcatcatgg ccagatagag cgccgactcc gcggcaccga
    25621 ccaaaagcga cgtggccaag tcgtagcgca tgccgaccga caccgccgac acgacataga
    25681 caacccagaa gtcgctcgcg gccccgcccg tgccgaggat ccacagcgcg ataaggatcg
    25741 agtcgctcac gatcgtcgcg gccccgaagc gcagcagggg atagcgctcg taggggcgaa
    25801 agaccaagga ccagatcgca taggggaccg cgatcgcgat gatggtccag gccagcgccg
    25861 gccgtggggt gttcccctgc atgaacagca gatagacgac gacgttgact cccacgacgg
    25921 cggcacgcag gatcgagacg cttcgctcca tggcgagcgt cttggcttgc gcggctgctt
    25981 ggagggccgc cgcgggcgtg gaagcgggca aaggatcctt tccgtgaagt tcgctcgcgc
    26041 atggtctcgg atcggaacgc gatagtcccg aatagtctcc gcgagccatt tatcggcgcg
    26101 gcgagcccgg cgcccgatcc tctcccgcga acagggggga ttcttcgtca cattccggac
    26161 attggcctca gcagtggatc ctagttgcgc agggggggtc cgtaccgtat aaaggctcct
    26221 tgcagcgcgg cgcacgtcgg ggccgcgcta ctactctgtc agggcccggt tcagcggggt
    26281 gagtgctaag gggaaggtgg attgtacgag cgccctacca aggtttgtct tgtctgggga
    26341 gccgccgagg gcgactccga gctaacggcg ttcgacaacg cgcttctcaa ggccggcatc
    26401 ggccacctca acctgatggc agtcacgagc atcttccccg aaggtgccgc actctccacg
    26461 cttcccgaga tccccacggg gaccctcaca cccacggtct acgccaagat ggtctccgag
    26521 acccccggcg aggtcatctc gtcgtgcatc ggcgtcggcc tgaccgatcg cggcggcgtc
    26581 ctgatggagg cccacggccc ctggaccgcc cagcagatcg aagagcgcgt aaccaagatg
    26641 gtcgccgaag gcatggccgt tcgcggcttc ggcgactacg agctccactt cgcgacggcc
    26701 gagcatcgag tcgatcgtat cggtagcgcg gttgcggccg cggttctttg gagggggtga
    26761 atggcgatga acgctctcgg gcggcacttt ctcgtcgagt tgtgggaagc gacgaatctg
    26821 gaccagccgg cagtgatcga agaggcactg atggaagcgg tacgcgtctc cggaggcacg
    26881 cttctggata cgaagatcgt cccgtttccg aacggggctt gctccgggat cgcgatcatc
    26941 gccgagtccc acctgtcgat ccacacgtgg ccggagttcg gctacgcggc cgtcgacatg
    27001 ttcacgtgca accacgacat ggacgccgac gcgggcttcg acgtgctccg caagtacttc
    27061 tcgcccggcc gaatccaggt tgtcgaagtc aagcgcgggc tgagcgcgtg acccggttcc
    27121 acgaagagcc tgctcccggt ctggagcagg cctaccaggg tacgcttttg cacgccgagg
    27181 aatcggcgtt ccagaaaatc gaggtctacg agcatccgac gttcggtcgg atgctcgtcc
    27241 tcgacgggct cgttcagacc acgacgcggg acgagttctg ctaccacgag atgctcgtgc
    27301 acgtgccgat gatcgccgtg gccgagccgc ggcgtgtgct gatcatcggc ggcggggacg
    27361 gcggcacgct tcgccgggtg ctcgagcacg ccaccgtcac cgaggccgtg atggtcgaga
    27421 tcgacgagac ggtcaccacg gtcagccgcg agttcttccc cgagatcgcc ggggacgcct
    27481 tcgacgaccc gcgcgcgacc gtgctcttcg acgacggcgc cgcctatgtg cgcgacggaa
    27541 gcgagccttt cgacgcgatc gtgatcgact cctccgaccc ggtcggcccg ggcgtggtgt
    27601 tgttctccga ggagttctac gggcagtgcc gggtgcggct gaaccccggc ggggtgctgt
    27661 cggcccagat cggctcgccc ttttacttcc cggacgagat cgcgatggcg ctcggcaacg
    27721 ccgccaaggc gtttccgcag gtcaagccat acgtcggcgc ggtgcccacc tatccgggca
    27781 cgctctgggg ctacatgctg gcgggggagc gcatcgacac cgacgcggac acggccggca
    27841 agcgcgcggc cgagcgaggc ctgcagcccc gctactggac gcccgagatc caagccggcg
    27901 cgttcgcggt cccggcgttc gtcgccgagg ttgcttcggg ctcgccggtg ttcccgaagc
    27961 cgtaggctgg tg
//
LOCUS       DATLFO010000255         5024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88054, whole genome shotgun sequence.
ACCESSION   DATLFO010000255 DATLFO010000000
VERSION     DATLFO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88054"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..671)
                     /locus_tag="VM841_10410"
     CDS             complement(144..671)
                     /locus_tag="VM841_10410"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL90631.1"
                     /translation="MSESEPRDGNVTGIMSKIRRGRREAAATASIPAVPTDVFRVITD
                     VENLPRWWPRVKKAMLIDGDELGREQVLTLDWGRQEGTIRQKVVEWEPGRRYAWTVVS
                     ESADGRELPPLVKTRVTVTIERDGMSGSRVEIRAHFDPVGGRGVLALRQISRLARASY
                     RKALRDLRGVVAPGA"
     gene            764..1036
                     /locus_tag="VM841_10415"
     CDS             764..1036
                     /locus_tag="VM841_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HVL90632.1"
                     /translation="MKQISATDAARGFADLINRVRYQGETYEVVRNGETVARIAPTVS
                     ARALTAAEMDDLLRNLPPLDAAFGKDVAVARDASRSDRERIGEWER"
     gene            1024..1428
                     /locus_tag="VM841_10420"
     CDS             1024..1428
                     /locus_tag="VM841_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90633.1"
                     /translation="MGTLIDVDTLIAAEAGFVALRDVLGSSEPVGIAAPTATELLETV
                     ERTDDPALRAHRASFVERLLERVDVVPFDGPAARTRARLEVVRPAGVDDRAMDVAAIA
                     VSRGWAIATGNGRYDRIPAVTVRKIEASETSA"
     gene            1443..2516
                     /locus_tag="VM841_10425"
     CDS             1443..2516
                     /locus_tag="VM841_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL90634.1"
                     /translation="MARHDDDDDLPRGSKVDDDIELDDAELLAQAAEDSEEDSSEDDG
                     KVVVRPEDADRLGDLDALGTYLREIGRYPLLNAAQEVELAKRIEAGAKAAKRLAKGDG
                     LTPAKKRELERVRRDGEAAKHDMVQANLRLVVSIARRYRATGIPLLDLVQEGNIGLMR
                     AVDKFDWRKGFKFSTYATWWIRQAIQRGIADRGRAIRLPVHVHELLVRVRRARAELEA
                     QYGREATDDEVAKSARLPVARVRELRGLAANLLSLETPVGEAGEATLGEFVPDEAAAG
                     QFDEVLSGIGREELEKVLSTLTERERTILALRFGLTGEEPLTLEEVGKRFGLTRERIR
                     QLEAKALAKLRHPSRSAALRTET"
     gene            complement(2576..4918)
                     /locus_tag="VM841_10430"
     CDS             complement(2576..4918)
                     /locus_tag="VM841_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVL90635.1"
                     /translation="MRSLRIVTALACLLTLAQAGPAAAEDGVPSADTHVAVTVAPEWG
                     TVGYPLEYRIAVVNDGPDEAAGAVLRFTIPWQTRMIGHDAPGSCSGAAPVVCGLGSLP
                     SGQSLTVTVRVRAATPGPAHFDAQINTDTADPATANNLTRTTVSISAPEYGVWRKPAD
                     SPTVGWGAAGASDPNTRHAVLFGGEINDDGDLSADTWRWNGSGWSLASVAAAPPARAM
                     SAAAFSGAGGNTVLFGGASDTAFLNDTWTWDGTAWAPAAVSGPSSRAGHALAARDGKV
                     VLFGGAGGTGLLNDTWEWDGSSWTKKTPAVSPPARMGHSLIEDRANGTVVLFGGCCAG
                     ESLHADTWIWDGATWKKAAPSASPPARADAAATFDPATNTATLAGGWDDDGETIGDVW
                     SWKDRRWVRRGHGPARDGAVLLHAADDGPGKLMMAGGADPDGRLSAGIPAATFAPDWR
                     VVSSTGPIAFGGVLAGATVTRSIVLRNLGTKDVSVPYAGVAGSAADGISADRSACSTL
                     APDGTCSIDVSFHAGLQTYMAAPRSATVTVWVNDSDETLSFPVTGTRQMVNLSTHALA
                     STVSVNAAFPVSWSAPGAAGVVYDVEYSERVYRSYAWREAEWKRWRTGTTSTSGWFGG
                     AGQPPSGQGKTMLFRVRARRGSEVSQWTPVRSTLVPIDDRNWTMKFSSGWSSVSASGR
                     FYGTLRQTSSAGKRFSFLFETNRLMIIGDKCPSCGAFDVYVDRVKVARVDSYAKGTLA
                     RQVLFDKYFSNGIKRHQLEIVTAGTRGRPWVKIDGVAAAR"
BASE COUNT          822 a         1784 c         1689 g          729 t
ORIGIN      
        1 ccgtgtgtcc acgccgtgtg tccacatggc cgccggccga ctcgtcaatt ttcttgacga
       61 gtcggccggc ggccatgtgg acacacggcg tggacacacg gcgtggacac acggcgtgga
      121 cacacggcgt ggacacacgg ccttcatgcg ccgggcgcaa cgacgccgcg cagatcccgc
      181 agggccttgc gataggacgc tcgggccagc cgcgatatct ggcgaagggc gagcactccg
      241 cggccgccga cgggatcgaa gtgcgcgcga atctccacgc gcgagccgga catgccgtcg
      301 cgctcgatgg tgacggtcac gcgcgtcttg accagcggcg gaagctcgcg gccgtcggcg
      361 gattcgctca cgaccgtcca ggcgtagcgg cgaccgggct cccattcgac taccttctgt
      421 cggatcgttc cctcctggcg gccccagtcg agcgtgagca cctgctcgcg ccccagctcg
      481 tcgccgtcga tgagcatcgc cttcttcacg cgcggccacc atcgcggcag gttctcgaca
      541 tcggtgatca cacggaacac gtccgtaggg acggccggga tcgaggcggt cgccgccgcc
      601 tcccgccggc cgcggcggat cttggacatg atcccggtca cgttgccgtc gcggggctcg
      661 gattcgctca tggggcctta tcgttccata cggaccgcgg ggcgctcgac tcgcgaacgc
      721 gtaatccgtc aatctgattt tctgattctg gtatgcttca tgggtgaagc agatcagcgc
      781 caccgatgcc gcccgcggct tcgccgacct catcaatcgc gtccgctatc agggcgaaac
      841 ctatgaggtc gtgcgcaacg gcgaaacggt tgcgcgcatc gccccgaccg tgtccgcccg
      901 cgcgctgacc gcagccgaga tggacgacct gcttcgcaac cttcctccgc tggacgccgc
      961 gttcggcaag gacgtggccg tagcccggga tgcttcccgc tcggaccggg aaaggatcgg
     1021 cgaatgggaa cgttgatcga cgtggacaca ctgatcgccg ccgaggccgg tttcgtggcg
     1081 cttcgtgacg ttttggggtc ttcggagcct gtggggatcg ccgcgccgac cgcgaccgag
     1141 ctgctcgaga ccgtcgagcg caccgacgat ccggcgctgc gcgcccatcg ggcctcgttc
     1201 gtggagcgcc tgctcgagcg agtggacgtg gtaccgttcg acggcccggc ggcgcgaacg
     1261 agagcgcggc ttgaggtcgt tcgcccggcc ggggtggacg atcgggcgat ggacgtggcc
     1321 gcgatcgccg tctcgcgcgg atgggcgatc gcgacgggaa acggccgcta cgacaggatc
     1381 ccggcagtga cggtacgaaa gatagaagct tcggaaacga gcgcatgagc ccaagggaga
     1441 gcatggccag acacgacgac gacgacgatc tgccgcgcgg cagcaaggtc gacgacgata
     1501 tcgagctcga cgacgcagag ctgctcgcgc aggccgccga ggactccgag gaagactcct
     1561 cggaggacga cggcaaggtc gtcgtccggc ccgaggacgc cgaccggctc ggcgacctcg
     1621 acgcgctcgg cacctacctg cgcgagatcg gccgctatcc cctgctcaac gccgcccagg
     1681 aggtcgagct ggccaagcgc atcgaggccg gcgccaaggc cgccaagcgg ctggccaaag
     1741 gcgacgggct gacgccggcg aagaagcgtg agctggaacg agtccgccgc gacggcgagg
     1801 ccgccaagca cgacatggtc caggctaacc tgcgcctggt cgtgtcgatc gcgcgccgct
     1861 accgcgcgac cggcatcccg ctgctggacc tggtccagga aggcaacatc ggcctgatgc
     1921 gcgccgtcga caagttcgac tggcgcaagg gcttcaagtt ctcgacgtat gcgacctggt
     1981 ggatccgtca ggcgatccag cgcggcatcg ccgatcgcgg ccgcgcgatc cgcctgcccg
     2041 tccacgtcca cgagcttttg gtccgcgtcc gccgcgcccg ggccgaattg gaagcgcagt
     2101 acggacgcga ggcgaccgac gacgaggtgg ccaagagcgc gcgcctgccg gtcgcacgcg
     2161 tacgcgagct gcgcggcctg gccgcgaacc tgctctcgct cgagaccccc gtcggcgagg
     2221 ccggcgaggc cacgctcgga gagttcgtgc ccgacgaggc cgccgccggg cagttcgacg
     2281 aggtcctgtc gggcatcggc cgcgaagagc tcgagaaggt gctcagcacg ctgaccgagc
     2341 gcgagcgcac gatcctggcg ctgcgcttcg gcctgaccgg cgaagagccg ctcacgctcg
     2401 aagaggtcgg caagcgcttc ggcctgacgc gcgagcggat ccgtcagttg gaagccaagg
     2461 cgctcgcgaa gctgcgccac cccagccgca gcgccgccct gcgaaccgag acctaatcac
     2521 ccgcaaagcg aacgggccgg tggaaatcca ccggcccgcc gtttttcttg tccgcctatc
     2581 gcgccgcggc gacgccgtcg atcttgaccc acggacgtcc acgcgttccg gccgtgacga
     2641 tctcgagctg atgccgcttg atgccgttgg agaagtactt gtcgaacagc acctgccgcg
     2701 ccagcgtgcc cttggcatac gaatccacgc gcgcgacctt gacccggtcc acgtagacgt
     2761 cgaacgcgcc gcacgagggg cacttgtccc cgatgatcat cagccggttc gtttcgaaca
     2821 ggaacgagaa acgcttgccg gccgacgagg tttgccgcag cgtgccgtag aagcggccgc
     2881 tcgcgctcac gctcgaccag ccggacgaga acttcatcgt ccagttgcgg tcgtcgatcg
     2941 gaaccagcgt cgagcgcacc ggcgtccact ggctgacctc gctgccgcga cgggcgcgca
     3001 cgcggaacag catcgtcttg ccctggccgc tgggcggctg gccggcgcca ccgaaccagc
     3061 ccgaggtcga tgtggtcccg gtccgccacc gcttccactc ggcctcgcgc caggcatagc
     3121 tgcggtagac gcgctcgctg tattcgacgt catagaccac gcctgccgcc cccggcgccg
     3181 accacgacac cgggaacgcc gcgttgaccg acacggtgct ggccagcgca tgcgtgctca
     3241 gattgaccat ctgccgcgtg cccgtcaccg gaaacgaaag ggtttcgtcg ctgtcgttga
     3301 cccaaacggt caccgtcgcc gaccgtggcg ccgccatgta cgtctgcaga ccggcgtgaa
     3361 agctgacatc gatcgagcag gtaccgtcgg gggccagcgt ggagcatgcg gagcggtcgg
     3421 ccgaaatgcc gtccgccgcc gaacccgcga cacccgcata cggcaccgaa acatccttcg
     3481 tcccgaggtt gcgcagcacg atcgaacgcg tcaccgtcgc acccgccagc accccgccga
     3541 acgcgatcgg accggtgctc gacaccacac gccaatcggg tgcgaacgtc gccgccggga
     3601 tgccggcgga caggcgccca tccgggtccg ctccgccggc catcatcagc ttgcccggcc
     3661 cgtcgtcggc cgcgtggagc agcaccgcgc cgtcacgagc gggtccgtga ccgcgccgaa
     3721 cccaccggcg atccttccac gaccaaacgt cgccgatcgt ctcgccgtcg tcgtcccagc
     3781 cacccgcgag cgtcgcggtg ttggtcgccg ggtcgaacgt tgcggcggca tcggcgcgcg
     3841 ccggcggcga cgcggacggc gccgccttct tccaggttgc gccgtcccag atccacgtgt
     3901 cggcgtgcaa tgactcgccg gcgcagcagc cgccgaacag caccacggtc ccgttcgcgc
     3961 ggtcttcgat cagcgagtga cccatgcgcg ccggcggcga cacggccggc gtcttcttcg
     4021 tccacgacga gccgtcccac tcccaggtgt cgttcagcag accggtcccg ccggcaccac
     4081 cgaacagcac gaccttgccg tcgcgggccg cgagcgcgtg acctgcacgc gacgacggac
     4141 cgctcaccgc ggccggcgcc catgcggttc cgtcccacgt ccaggtgtcg ttgagaaacg
     4201 cggtatcgga ggcgccgccg aacagcacgg tgttcccgcc ggctccgctg aaggcggccg
     4261 cgctcatcgc gcgcgccggc ggggcggcgg caacggaggc caggctccag ccggatccat
     4321 tccatcgcca ggtgtcggcc gacaggtcgc cgtcgtcgtt gatctcgccg ccgaacagca
     4381 ccgcgtgacg cgtattcgga tccgacgcac ccgctgcgcc ccatcccacc gtcggagaat
     4441 cggccggctt ccgccagacg ccgtactcgg gcgccgagat gctgaccgtc gtgcgggtca
     4501 ggttgtttgc cgtcgccggg tcggccgtgt cggtgttgat ctgcgcatcg aaatgcgccg
     4561 gtcccggcgt ggcggcacgg acccgaaccg tgaccgtcag gctttgaccc gacggcagag
     4621 agccaagacc gcagacaacc ggcgcggcgc ccgagcagga tccaggagcg tcatggccga
     4681 tcatccgtgt ctgccacggg atcgtgaaac gaagcacggc cccggcggcc tcgtccggcc
     4741 cgtcgttgac cacggcgatc cgatactcga gcgggtatcc gacggtgccc cattcgggcg
     4801 caaccgtgac cgcgacgtgg gtgtcggcgg acgggacccc gtcttcggcg gctgccggtc
     4861 cggcctgggc aagcgtgagc aggcacgcca aagcggtgac gatacgaagg gaacgcacgg
     4921 gaatcctttc ttcggggaag aatgcgtccg gattatcggc agtccgcgca ccgcggaccc
     4981 ataaataccg gataaggttt ccttagcagc aagcagaatt gtgg
//
LOCUS       DATLFO010000256        25841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_64798, whole genome shotgun sequence.
ACCESSION   DATLFO010000256 DATLFO010000000
VERSION     DATLFO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64798"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VM841_10435"
     CDS             <1..1857
                     /locus_tag="VM841_10435"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVL90636.1"
                     /translation="VYQETVNPPTAGTANSPVTFRSLGGTVVTGGTRGFLIRRNWIVV
                     EGFEIASTSSHGIYATGAAFVTIRGNYVHHVGQQTKTDTAKGIYLKDVTDSTIEGNTV
                     DQATDAGIAVFGASARVIVRGNTSNRNARGYVRAAVGIDIRNASVVTVENNRLSENED
                     SGINVWDSADVIVRGNVASRNGDHGIDNKASINTAIVANTVYGGVDSGIEVVGTATGV
                     TVMNNISVDNGINSPRTTGNLRVDGTALGAVQLDFNLVWLSQAGDMIVWGNDRYSTLA
                     SFVSATGQMANGKETDPKFANAGTGDFRLLPGSAAIDAANANAPGQAPVDSTGQSRVD
                     DPATPNTGAGVRAFDDIGAFEYVPSGPADQPPVAVSDSATVQEGSAATVIDVLANDTD
                     PDGGPKSVGSVTQPVNGTVTIGQDGAHVTYAPKAGYCNTGSGPDTFGYTLTPGGSSAT
                     VSMTVTCAQQTPVLLILLPAGDATIRQARPTSANGSGTVLSVDGDPVKDVLIKFAVSG
                     VGTKTIKRAAIKLWCVDSSGTGGTFHTVSSSSWDENTVTWATAPSFDPTPFRTMGPVE
                     SGNFYEVDVTDKIIGDGDYSFRVDLISIYDAGYSSKEEPDDGRRPRLIIEFQ"
     gene            complement(1861..2787)
                     /locus_tag="VM841_10440"
     CDS             complement(1861..2787)
                     /locus_tag="VM841_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90637.1"
                     /translation="MCDLSRAVSLTGEHQDAIKYDHADPGTVFDARNAIITAYPHRAV
                     YPFSVGGAAAPERICVVGGIVAGDAPRGLTWAEMKDLFDGDGLRIAGNGWYGVNGLRV
                     DNVSDGIAPRGTKDLYPADGDGFEFRNLYFTYIRDDCVENDEIAAGAIYDSLFDGCYT
                     GISERPSSDSPQWRYPPPPGETLLLDGVLLRLEAMPGPRGAGGTATGGHGQLFKWSEV
                     ANRLTIKRSIFLVEQEPNSTSFFPFPPGTTTEDVTIVWLGDSTFTWKVPSGTIVTTDR
                     AVWDEARADWLRRHGCSSFLECSRLYDPAPPG"
     gene            complement(2974..3912)
                     /locus_tag="VM841_10445"
     CDS             complement(2974..3912)
                     /locus_tag="VM841_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90638.1"
                     /translation="MNRARQFIMVAAAGLSLVAASCSTAGTGTAPASAKLPVAAASEP
                     AAPHPVNFLRESFGPDSATITNPWWPLKPGTQWIWEGTAIEGEDEIERRVVFTVTDLT
                     KTVAGIETIVGWDRDFTDGVLAESELIFLAQDRAGNVWHFGQQVERYDETGELEGGRT
                     WLVGYLADAKAGIKMKANPTPGDPVYSQGFAPPPFYWDDVARAVEIEDRMCVPVGCYD
                     NVLIIEEFEPTKPGAKQLKYYARGIGNIAVGWGGEAELEQEEMELTRMLTLSPAGMAE
                     VREAARSIETRGNVYGLTSPLRQRPAAATASPSPSP"
     gene            complement(3944..7072)
                     /locus_tag="VM841_10450"
     CDS             complement(3944..7072)
                     /locus_tag="VM841_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL90639.1"
                     /translation="MLRWIVGTSLRFRFLIVAGAAALMVFGVGQLKEMPIDVFPEFAP
                     PRVEIQTAALGLSPAEVEALVTVPLEEALQGLPSLDAIRSKSVEQLSSIELIFNPGTD
                     LLHARQVVGERLASVTPTLPTWSAPPFMMQPLSATSRIMKIGMSSVDHSLIDMSLLAY
                     WKMRARLLRVPGVANVTIWGERVSQLHVEVDPGKLGAEKVSLQRVMEVTAEALDAGIL
                     QFSDGHVIGGGGFVDTPNQRIGVRHILPILTPDQLAKIPIASRDGRTLRLADIANLTE
                     GHQPLIGDAVINDGPGLMLIVEKLPWGNTLEVSRGVEAAIEEMKPGLPGIEIDTTIFR
                     PATFVEHAVENLGRSLLLGVLLMVIVLGAFLYDWRSALISAVTIPLSLTAAGLVLWAR
                     GATINTMVLAGFVIALGAVVDDAIVDVENIVRRLRIHRAAGGDRSLPTTARVIYHASL
                     EVRGAVVYASIIEALALLPVFFLTGLTGSFFRPLAMSYALAVVISTIVALVSTPALSL
                     LLLRKADLEKRESPVVRWVHGVYERALAPIVRSPRPAYAAVGGLVALGLGVTPFLGQD
                     LLPSFKERDFLMHWVTAPGTSLPEEMRVTVAASKELRDIPGVRNFGAHIGQAFLADEV
                     VAVDFGENWVSVDPKADYDKTLAAIQSVVDGYPGIRRDVQTYLKERIREVLTGAGEAI
                     VIRIAGPDLDELQRIGKEIETLIGEVPGVVEEHVELQKRIPQIAVTVDLDAAGRVGLK
                     PGDVRRAAAIYMASEEVGDILKDGKAYDVHLYSTPETRTDLTAVRALLMDTPSGGQVR
                     LDDVADVSLRSTPNSIHREGVTRVIDVEANVKGRDLGSVVAGIEQKLRGYEFPQEYHA
                     EILGEFAERQEAQSRLFGWAIVAAIGIVALLVTSFASMRLAILTFLTMPIAMVGGILA
                     ARMSSGIISLGSLVGFFTVLGIVARNGIMMISHFRHLEIQEGVPFGPGLVLRGARERV
                     APILMTALTTGLALVPLLITGNIPGQEIEYPMAIVILGGLVASTSLNLFVVPSLYLRF
                     ARTEKLRARTAG"
     gene            complement(7091..7513)
                     /locus_tag="VM841_10455"
     CDS             complement(7091..7513)
                     /locus_tag="VM841_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90640.1"
                     /translation="MNAKRLIAAPVVVLLALTACRSVPEQTGGRSPANVEAIEGSEVS
                     RITLTGRAAERLGIETNPVRPSSGSRTVVPYSAIYYDAEGDTWVFVESDGGYARAPVV
                     VEAIEGERAILTAGPVPGTPVVVVGVAELHGIEAGIGQ"
     gene            complement(7523..10786)
                     /locus_tag="VM841_10460"
     CDS             complement(7523..10786)
                     /locus_tag="VM841_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVL90641.1"
                     /translation="MFRWMVGSSLRARGLVIAIAAGVMFLGAQQLRSMPADALPEFTQ
                     PTVHIQSEALGLSAREVEDLITAPFEQDLLNGVPFLTAIRSESMPGLSSIELVFEPGT
                     DIFRARQVVQERLAEAVVALPGASRPPQMLQPVSSTGRAMIIGLSSEQLSLIELSVLA
                     RWTVKPRLLGVPGVANVSVWGNRDQQLQVHVDPERLRAHEVTLQQIIETTGNALWVSP
                     LSFLEASTPGTGGFIDTPNQRLAIQHLLPVRTPEDLAQIAVDDTGPQPLRLGDVATVV
                     EDHQPLIGNALLAGGPGLLLVIEKFPEADILEVTRHLDEALETLRPALKGVTVDASLF
                     RPAASIERATSTVGRALLIGFVLMIVMLFLVFMGWREALIVAITVPLSLLATAAIMGL
                     TGLTLNAMTGAGLVMGLALVIDDAITGVDAVVRRTRRGEARPGGLGVRAVHDTVLAVR
                     SPLAYGTAIVVLAVMPLFLLDGVAGAFLPSLAWTFALAAITSMIVALTVAPALALALL
                     GRASAKPDTAAARGTRGLVARMLPRFLRSRTPLIVALVVTVAGFAVLPFLRHSSDPGI
                     QERHFAIDLEAAPGTSLPEMTRITQRMAREIRGVDGVDSVAIQVGRAITSDRVVNVNA
                     GELWVSVSPSAPYRQTIDRIRSIVAGYPGFDQDIGTYTKERVTDELSGVEHPVTVRLF
                     GSDLDVLREKADEVRSAIAGVEGVEDAAVSAVPTEPTVEVEVDLTAAETLGIKPGDVR
                     RAAATLLAGIHVGSLFEEQKVFDVVVWSSPETRYNISSLQDLLIETPEGRPVRLGDVA
                     SVHLAPNPTVVRHEAVSRTMDVTAGVRGRGLGAVLADIERRIDGIAFPLEHHAEIMGD
                     FDTQQAGKREFIVFAIAAAIGALLIAQASVRSWVLALLAILLAGASLAGGAVAVLLTD
                     GIVSLGSIAGFLLLFVLALRHLLGLLDRYHRLRYDGVAFDRRLAVRGAIDRAVPIAAS
                     TLAIAVVTLPLTLVTPVEGFEIIRPMAIVLLGGVFVAAALTLFVMPGLYLLAGRALEP
                     DTGTYFVGEGGGEHRVIVLDEPAAESARVPVQPPPAAEGTVMTKHEPDGDGHHTREP"
     gene            complement(10807..11661)
                     /locus_tag="VM841_10465"
     CDS             complement(10807..11661)
                     /locus_tag="VM841_10465"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVL90642.1"
                     /translation="MTLPNFVVVGAMKTGTTSLYHYLRAHPEVFMPRVKELDFFLAEA
                     NWERGLRWYERRFAAVPPRAKAVGEASTNYSKFPQFTGVPERMAAVIPHARIVYVVRD
                     PVDRILSHYRHRVAVGSERRPINVAIRSDPTYINASRYAMQAHRFLRHYPRERMLIMA
                     SEALKNDRSTVIEAVYRFLGVDPSYRAPVHTQEFYRTDARTIHSPIAWHLRTTIKRRF
                     PAAKEAKELVDAMRGAIRHNAHRPAARDRETAMPDALRRELTGRLIEDTECLRALAGD
                     IVGSWDLT"
     gene            complement(11658..12434)
                     /locus_tag="VM841_10470"
     CDS             complement(11658..12434)
                     /locus_tag="VM841_10470"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HVL90643.1"
                     /translation="MAAARSHSDVRPDIGRGIGPSVARRVFDVIVATVALALLLAPML
                     LVALAIRSTSRGPALYGQMRIGQGGRPFRVLKFRTMRRDSSGSALTVPGDTRVTRLGR
                     VLRATCIDELPQLLNVLMGQMTLVGPRPQTPGFAAEYPPDLRVIFRYRPGVTGPGVIT
                     FNDEDTLPPATATTDEIEAFYLENVVPRRVAVDLEFCRNATVGRTVRVLWQTVLTARH
                     RFCARKPEQQRVITLPDSRYEPADFTGTAAEVAEQRSQAG"
     gene            complement(12347..13477)
                     /locus_tag="VM841_10475"
     CDS             complement(12347..13477)
                     /locus_tag="VM841_10475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HVL90644.1"
                     /translation="MIDAIVDWLASGVPQVPRVRGGIVAGMYRTAPKVTLMLFDEAGD
                     PAVVVKVARDVAAEPRLRAEHEALLRFGPDGPHGAFAPRPHAIGRVAGRLALAEEAFT
                     GRPMTTTYYEPGHVSSRRRVEADFACAGAWLDSFAWATADRAARLGDREIERFVEEPI
                     ARYRAEIGWSPEEAALFSYARAAAERMRGERLPLSGVHGDFWMGNLLVTGGRISGVVD
                     WELAREAVPPFRDIYKFPSSYAFYLDRGMGVRGRVPEHHRYLNGARIASPYTHWANAA
                     GFAYAFMSGGWFASLARRWINDRLAMHAIDASFNASFFPVFLAEQAVTLDDITIRAGY
                     RSLVRLLGSGRRASWLLPDHTPTSGRTSAAASDRASHAASST"
     gene            complement(13474..14085)
                     /locus_tag="VM841_10480"
     CDS             complement(13474..14085)
                     /locus_tag="VM841_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90645.1"
                     /translation="MSAIRDVRRMNWWYVLPPATGPVLLVGDAAHMDAFSDPIIASGL
                     DESLRAVAATRYGGIAVPDLDAVFHAGPSEFDGLRTLMAAVEPGGWFYGGFSNRPLRR
                     KGLSLGRVRTLLHLAGMRDHRVFLAAPDHVTPAYLIPAARRAEMDFFLRMFFFPYSRA
                     SGTRAAVRRMALRFLRVGTSVSPAWLRARVWPSVAVVARKEHP"
     gene            complement(14082..15554)
                     /locus_tag="VM841_10485"
     CDS             complement(14082..15554)
                     /locus_tag="VM841_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HVL90646.1"
                     /translation="MTFPQPDIVPRGLGLRRIAGNSAWQVVSFTFRGLAGLGVVVALA
                     RVEGPGRLGVFQFALTLTSLLPYYWGYPALLSREVARRPGHVRGWLEGGMAMTFVFGI
                     AFSIALTVGARTFGASPESQMTITLATTAMIFDGIARIQFAAFVAWERMRLETIATAL
                     QETMFLVGAVIAVGLGGGPVGAMIAFLASRFVGALIAWITASLRCRTLLLPAWHGGFA
                     VRSAVSATPFAAADTLKLVQMRGDAVLLGLMKGPAAVGLYQACTSLVMHWNVLARSLN
                     HAVYPRMSRAWPAHPTRFARYRDASNRSLAMIGLPVAIGGALLAADILHALYGPKFAR
                     ATLAFQILVLVVPIRMMSNTLSLSLAACDRQRQRTKAIALAASANLTLNLALIPIWSY
                     LGSAAATLATETGLLVGYALQLRPAIGSSRMMQAIALPATACVPLAAAVMATRSAPLV
                     VPMLAGALAYGGTLTLAAMIRFRHESRSPVAALIGLVRPT"
     gene            complement(15551..16846)
                     /locus_tag="VM841_10490"
     CDS             complement(15551..16846)
                     /locus_tag="VM841_10490"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL90647.1"
                     /translation="MSLPDSVWASEPEPATPAASLPPGPAAPRIKVLHVITKFTTGAG
                     GNTLHSATGMDPARYEVWVAARPGGECFEPARRAGIRTVEIPRLAETIRPLDDWLAYR
                     ALVRLIKRERFSIVHTHTAKAGLIGRMAARRCHVPVVIHTFHAFPFHDHMSRLRRRVY
                     LAIDRTARRSTHAFLAVAPQVARDAVENRLAPPGTVRVAPSSVELADIPRGRDRAFRD
                     EIGIGEDVPLIGTVGRITFQKAPKDFVRAAAAVAAVRPDARFVMVGDGPMEAEARAEA
                     ARLGIDLLFTGFRDDAHRIAAAFDVFVMPSLYEGLGRALTEALASGRPVVASAVNGVA
                     DLVRPGATGLLSRPGDPAHVAECILWMLEHPSEARRMGIHGRNVVRSLFSQQRMCEIL
                     DRTYRSLLGMPTEELRARPVRLSDAHGNGARPAQRVIAT"
     gene            complement(16843..17790)
                     /locus_tag="VM841_10495"
     CDS             complement(16843..17790)
                     /locus_tag="VM841_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL90648.1"
                     /translation="MNALVTGAAGFVGSHLVEHLLESGDLVRGVDCFTGYYDRAAKES
                     NVRDASAHPRFTFVEADVAATRLTDLLTGIDAVYHLAGQPGVRPSWGRDFDHYTRQNI
                     TTTQRLLEAARSTPLRSFVYASSSSVYGDADAYPTAESALPRPLSPYGVTKLAGEHLC
                     ELYRSAFGISTASLRLFTVYGPRQRPDMAFARLMSAARTAGTFELSGDGTQTRDFTDV
                     SDVVFAMRRVAVTEWRGVANIGGGAQHSMNEVVAAIETITGRDISVVRRRSARGDVKH
                     TGADVTRAREGFGYRPRVALTDGLAAMWAWAREQEAAVA"
     gene            complement(17787..19070)
                     /locus_tag="VM841_10500"
     CDS             complement(17787..19070)
                     /locus_tag="VM841_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL90649.1"
                     /translation="MNPAAPTVYRVPRMPWQRTGEPPAALLRIVMFAIAVIPLVKPRL
                     PGNSGPADFFMVTATIAVFVWIAAEGRAIFAPFAGATAVLMTAGAISGLVADRAGPTL
                     LVMLQEAHLLLFTAAIATVARTPRAIEEIRSAWVAAGVFWGALVVAAVASGFAPLINA
                     TTDRGTRAHLFFQNPNITGNYFLIAIFVAVASRPKSWPRRSAAVAVMLAAMLLTGSNA
                     AILGLAAGAVVAVFVYAWRRIDPIVAVVVLSAILVAGAGSYVAMGESFRRAPESENLL
                     IRYSIGRLERSAANRQDLFSEQMALYRERGILGIGPFATRDELGRSGESGVRSSHNDY
                     LATLVERGPIGVAGLLLLAGAIGYRIARGHAGVVKPEWRKAAGTGAAMTGVAVALALS
                     ALTHEILHYRHLWALLGLIAAVHRWSHQDAKGGAP"
     gene            complement(19067..20476)
                     /locus_tag="VM841_10505"
     CDS             complement(19067..20476)
                     /locus_tag="VM841_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVL90650.1"
                     /translation="MTLARPDTSIPRIFAGDESPPDAAALAALMRGVRDADLAAPPVV
                     LPDFLHKPRMEMPSSIAVATRGTIRPAFTASSLNCGMTLLALDGGTAGPEDVVRFLSE
                     TRAAHPWPPGLNRTLGTGDVIAAATLGTEFAVDRYGLPATARARTEEGGRLDLDRFGG
                     PAALRREIPWLTRVLSGMRFAAVGPSNHFIELQRVEAVLDPPAARALGLSEGGLTIQF
                     HGGGGVLPGQIGRLYARRRKAPTTVRMTMALQKPGHHLCSARSIEQVRRRFALYFREG
                     AAIPLDGVEGRRVMLAHAAAMNFGFAFRGAVAASLAALARRTLGAPAVRPVVDSPHNS
                     IYEEDGAIVHRHNACRAWPAQRFEPGSPFAHTGQALLLPGTARTSSYVCVAGSGADAS
                     LYSACHGSGGIVADFISRGLSRPDPRPRATLRMRYDGRGPQAAGHYDDRGVDEALSIL
                     TRHDIARPVARLRPLGVLH"
     gene            complement(20473..21486)
                     /locus_tag="VM841_10510"
     CDS             complement(20473..21486)
                     /locus_tag="VM841_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90651.1"
                     /translation="MPGTIETAGERIRFDVAIGWVADLVEESTGSPLAPGSGDETLRV
                     VVERDRKPFPGDWDEAGRALARRDREVVIRNAAQSGFDLLVHAWHSPPELIFRWRPGI
                     ELRAFRAFPARWRLRLGAMLTQYPALWRASTAARPPLHAACVRIDQGTALVVGMPGAG
                     TSTVLAAEMAAGALPITDNLCVADARTVWGVAEPFRIEPWAVPDDVIALPTTRGRMSV
                     VPETRLPSARPDVVVLLERGRETRVREADPAELARLMVTSTYACGELRRFWPLAATLA
                     MTTGYGPALPPVLPVARALVDRLPCVGVALARPGIRLSDALGGYSARRSVPDARDRVA
                     ITR"
     gene            complement(21501..22826)
                     /locus_tag="VM841_10515"
     CDS             complement(21501..22826)
                     /locus_tag="VM841_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90652.1"
                     /translation="MRHARILIVLTIIGLLGALAIGATQEPRYEATSRVSLGGWDPND
                     GARAKTLADSARAIATSDLVVRRAISTIDQERDVGEVADSIVLTAAGSSSVVDITASD
                     KDPHAAASIANGIALALIDVRSNMLRGGVQTLLDDLDRRMAEADERNVTLEAAARAAA
                     PGTQAAQVALQQYTAAVRQRAELDVDRRQIVQIGAAQPRAAVLDSANVPASPSGTPHA
                     LRLMLGLVAGLLGGLAIAAASEVLRPTVMGSEAIAAAAGAPWLGTVPAGDPEDLDERE
                     ATVTRAIRLAATRADVRSVLVVPVGTEAGAADDLASSLKPEGEPESEPAGGPVPGSRI
                     AIVDRSAPSDGGIVAVAPEALHRKSLAVLHHVAAASGWPLLGFVLVQKKRGRRRHPQE
                     VVFKAVPRLEAEPASDDGFRGRHVPAFERASEAFMSGGKPKAGNGDGAS"
     gene            23037..23969
                     /locus_tag="VM841_10520"
     CDS             23037..23969
                     /locus_tag="VM841_10520"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVL90653.1"
                     /translation="MSTQPVPFVVGAARSGTTLLRAMLDAHPDLAVPGESHFIVRMLA
                     TRHVYEQPWGVDVHGYRRALARERYFAAWGLPAPDLRDALVRANPRSLEDAMRATFRA
                     YARRQGKQRWGDKTPSYVLHVRAIAGSFPEARILHVIRDGRDVARSMVDLGWAASHED
                     AVLRWAHRVRRGRKEGARLGERYVEVRYEDLVGDPIGTLTRLCVSLDLPFDPAMTQPH
                     RRADEILATLANPENHQRIRRPPSRVRDWRTEMDSESIRKIESVAGDLLTELGYELGG
                     PPATLPLRVAARSASLRSLPRRMGSRWRRTLLIR"
     gene            24039..24716
                     /locus_tag="VM841_10525"
     CDS             24039..24716
                     /locus_tag="VM841_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVL90654.1"
                     /translation="MLAFALATLAACTDTAAESLLDRVAPAASPGAPATEPPAAEPPA
                     TGPGPVAASPTPAAIPADPGVVIRVVDGDTAHIRLASGTIEKVRFIGVNTPESTTRHE
                     PYGKEASRFTTTRLDGRTVYLEYDVERRDRYGRLLAYIWMRPPSDGSPSDVRAHMFNA
                     TLLIEGYAQTATYPPNVRYADVFRDLQREAREQGRGLWALPSEDQGASRFPASSASSP
                     RSGCDPS"
     gene            complement(24748..25689)
                     /locus_tag="VM841_10530"
     CDS             complement(24748..25689)
                     /locus_tag="VM841_10530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVL90655.1"
                     /translation="MIAVSLLPSLPAGAVSRSELESRVATLKKTLDAIAVSLSAAEME
                     LDAAQKAVDVHTRALATAGKKLATLRTAHSRRSAEMYIAGSMGLIDTIVASQDVDVFV
                     ERLGYLERIRSAEEGTLESIVALQRRAKTESVELRQARDAAAAALRTLQKRRSGLDAK
                     LREWQSLLDLSKAMSSNTRASRSSIRGFRCPVSGPHGLSNDFGDRRRGGPHQGIDMPA
                     NSGTPLVAVLTGRVSDVVRGGWMGRGVILRDTAGNEWWYAHLSSTYVSRGESVTAGQI
                     LGRVGCTGNCTGPHLHFEYHPAGGAARNPYKILRAAC"
BASE COUNT         4116 a         8893 c         9137 g         3695 t
ORIGIN      
        1 gtctatcaag agaccgtaaa cccgcccacc gcggggacgg caaacagccc ggtgacgttc
       61 cgatcactcg ggggaacggt ggtgaccggc gggacacgcg gattcctcat tcgccgcaac
      121 tggatcgtcg tcgaaggctt cgagatcgcg agcacctcga gccacggcat ctacgccacc
      181 ggagccgcct tcgtgacgat ccgcggcaac tacgtgcacc acgtcggtca gcagaccaag
      241 accgacaccg cgaaagggat ctatctcaag gacgtcaccg attccaccat cgagggcaac
      301 acggtggatc aggcgaccga cgccgggatt gcggtgttcg gagcctccgc ccgggtgatc
      361 gtgcgcggga acacttcgaa ccggaacgcg cgcggctatg tgcgtgccgc ggtcgggatc
      421 gacatccgga acgcctcggt cgtgaccgtc gagaacaacc gtctgagcga gaacgaagac
      481 tccggcatca acgtctggga ttcggccgac gtgatcgttc gaggcaacgt cgcctcgcgg
      541 aacggcgatc acggcatcga caacaaggcc tccatcaata cggcgatcgt cgccaacacc
      601 gtctacggcg gggtggactc gggcatcgag gtcgtcggga ccgccaccgg cgtgaccgtg
      661 atgaacaaca tcagcgtcga caacggcatc aacagccctc ggaccaccgg gaacctgcgc
      721 gttgacggca cggcgctcgg agccgtgcag ctcgatttca acctcgtgtg gctctcgcag
      781 gccggcgaca tgatcgtgtg gggcaacgac cgctactcga cgctggcaag cttcgtgagc
      841 gccaccggcc agatggccaa cggcaaggag acagacccga agttcgcgaa tgcagggacg
      901 ggcgatttcc ggctgcttcc gggctctgcg gcgatcgacg cggcgaacgc gaacgcgccg
      961 ggccaggcac cggtggactc gaccgggcaa agccgggtgg acgaccccgc gacgccgaac
     1021 acgggggccg gcgtccgggc cttcgacgac atcggcgcct tcgaatacgt gccgtccggc
     1081 cccgcggatc agccaccggt cgcggtgagc gacagcgcga cggtgcaaga aggttctgcg
     1141 gcgaccgtga tcgacgtgct ggccaacgac accgaccccg acggcgggcc gaagagcgtg
     1201 gggtcggtga cgcagccggt caacgggacg gtcacgattg gccaagacgg cgcgcacgtc
     1261 acctatgcgc cgaaggccgg ctactgcaac accggctccg ggcccgacac tttcggctac
     1321 acgctcacgc ccggtggctc gtcggcgacg gtctcgatga cggtgacgtg cgcgcagcag
     1381 acgcccgtgc tgctgatcct gctccctgca ggcgacgcga ccattcgcca ggcacggccc
     1441 acctcggcga acggctccgg aacggtcctg tcggtcgacg gcgatcccgt caaggacgtc
     1501 ctgatcaagt tcgcggttag cggcgtggga accaagacga tcaagcgggc cgccatcaag
     1561 ctctggtgcg tcgattcgtc gggcacgggc ggcacgttcc acacggtgtc gagctcgagc
     1621 tgggacgaga acaccgtgac gtgggcgacg gcgccgtcgt tcgacccgac gcccttccgg
     1681 acgatgggtc cggtggaatc cggaaacttc tacgaggtcg acgtcaccga caagatcatc
     1741 ggcgacggcg actactcgtt ccgggtggac ctcatctcga tctacgacgc cggctactcg
     1801 tcgaaggaag agcccgacga cggccgccgg ccccgcctga tcatcgagtt ccagtgacgg
     1861 tcatccgggc ggagccggat cgtagagacg cgagcattcc aagaacgacg agcagccgtg
     1921 gcggcgcagc caatcggcgc gcgcctcgtc ccagacggca cggtcggtgg tcacgatcgt
     1981 gccggagggc accttccacg tgaaggtcga gtcgccgagc cacacgatcg tgacgtcctc
     2041 ggtggtggtg ccgggcggga acggaaagaa gctggtggag ttgggctcct gctcgaccag
     2101 gaagatcgag cgcttgatcg tgagccggtt cgccacctcg gaccatttga acagctgacc
     2161 gtgaccgccg gtcgccgtcc cgcccgcgcc gcgcggcccg ggcatggcct ccaagcggag
     2221 cagcacgccg tcgagcaaca gcgtctcgcc gggcgggggc gggtagcgcc actgcgggct
     2281 atcgctgctc gggcgctccg agatgccggt gtagcagccg tcgaagaggc tgtcgtagat
     2341 cgcgcccgcc gcgatctcgt cgttttcgac gcagtcgtcg cgaatgtagg tgaagtagag
     2401 attacggaac tcgaagccgt cgccgtcggc cgggtagaga tccttcgtgc cgcgtggcgc
     2461 gatgccgtcg gacacgttgt ccacgcgcag cccgttgacc ccgtaccagc cgtttccggc
     2521 gatgcgaagc ccgtctccgt cgaacagatc cttcatctcg gcccaggtga ggccgcgcgg
     2581 tgcgtcgccg gccacgatgc caccgaccac gcagatgcgc tcgggcgcgg ccgcgccgcc
     2641 gacgctgaag gggtagaccg cacgatgcgg atacgcggtg atgatcgcgt tccgcgcgtc
     2701 gaagaccgtg ccgggatcag cgtggtcgta cttgatcgcg tcctggtgct cgcccgtcag
     2761 cgagacggcg cggcttagat cgcacatcgg ctttgcgccc gggggatcgt cgggcggcgg
     2821 cgaaggcgtg gccgccggca gagagccggg gggtcgggcc gagggggaga tcgatggtgg
     2881 cccgccccgc ggggcttctt cggcgcccgt acaggccgcc gcggccagcg cgagcgtggc
     2941 cagtcgcgcg agtccccggc gccccgtcat cgttcacggc gacggcgacg gcgaggccgt
     3001 ggctgctgcc ggccgctgcc ggagcgggga cgtcaacccg tagacgttgc cgcgtgtctc
     3061 gatgctcctg gcggcttcgc gcacctcggc catgccggcc gggcttaggg tcagcatccg
     3121 cgtgagctcc atctcctcct gttcgagctc ggcctctcct ccccatccga cggcgatgtt
     3181 gccgatgcct cgcgcgtagt acttgagctg cttggcgccc ggcttcgtcg gctcgaactc
     3241 ctcgatgatg agcacgttgt cgtagcagcc gaccggcacg cacatgcggt cctcgatctc
     3301 gacggcccgc gcgacgtcat cccaatagaa gggtggcggc gcgaagcctt gcgagtagac
     3361 cgggtcgccc ggcgtcgggt tcgccttcat cttgatcccg gccttcgcgt cggcgagata
     3421 gcccacgagc caggtccggc ctccttccag ctcgccggtc tcgtcgtagc gctccacctg
     3481 ctggccgaag tgccagacgt ttccggcgcg gtcctgggcg aggaagatga gctcggactc
     3541 ggcgagcacg ccgtcggtga agtcgcgatc ccagcccacg atcgtctcga tgccggcgac
     3601 cgtcttggtg agatcggtga cggtgaacac gacccggcgt tcgatctcgt cctcgccttc
     3661 gatcgcggtg ccctcccaga tccactgggt gccgggcttc agcggccacc acgggttggt
     3721 gatggtggcc gagtccggcc cgaagctctc tcgaaggaag ttcaccggat ggggagccgc
     3781 cggctcggag gccgccgcga ccggcagttt tgcggatgcc ggcgcggtgc cggttcctgc
     3841 ggtcgagcag ctcgccgcga ccagactcaa gccggccgcc gccaccatga tgaactggcg
     3901 cgcgcgattc atctgtgtcc ctcctcggcc ttcttcgagc ccgtcatccc gccgtcctcg
     3961 cccgcagctt ctcggtccgt gcgaagcgca gatacaggga tgggacgacg aacagattca
     4021 gcgacgtgga tgcgaccagg ccgccaagga tcacgatcgc catcgggtac tcgatctcct
     4081 gtcctgggat gttgcccgtg atcagcagag ggacaagggc caggccggtg gtcagcgccg
     4141 tcatcaagat gggcgcgacg cgttcgcggg cgccgcgcag gacgaggccg ggtccgaacg
     4201 gcacgccctc ttggatctcg agatggcgga agtggctgat catcatgatg ccattgcgcg
     4261 ccacgatgcc gagcaccgtg aagaagccca cgagcgagcc gagcgagatg atgccgctac
     4321 tcatcctcgc cgcgaggatc ccgcccacca tcgcgatcgg catggtgagg aaggtcagga
     4381 tggccaaacg catgctcgcg aacgaggtga cgagcagcgc gacgatgccg atcgccgcga
     4441 cgatcgccca gccgaacagc cggctctgcg cctcctggcg ctcggcgaac tcgccgagga
     4501 tctcggcgtg atattcctgt gggaattcgt agccgcgcag cttctgttcg atgccggcga
     4561 cgaccgagcc caggtcgcgg cctttcacgt tggcttcgac gtcgatgacg cgcgtgacgc
     4621 cctcccggtg gatgctgttc ggcgtcgaac gaagcgagac gtcggcgacg tcgtcgagcc
     4681 ggacctggcc gcccgacggc gtgtccatga gcagcgcccg gacggcggtc aggtcggtgc
     4741 gcgtctccgg cgtgctgtac aggtgcacgt cgtaggcctt gccgtccttg aggatgtccc
     4801 cgacctcctc gctggccatg tagatggcgg cggcccggcg tacgtcgccg ggcttgaggc
     4861 cgacccgccc ggccgcgtcg aggtccaccg tcacggcgat ctgcgggatg cgcttttgca
     4921 gctcgacgtg ctcctcgacc acaccgggaa cctcgccgat gagcgtttcg atctccttgc
     4981 cgatgcgttg cagctcgtcg aggtccgggc cggcgatccg aatgacgatc gcctcgccgg
     5041 caccggtgag cacctcgcgg atgcgctcct tcagataggt ctggacgtcg cgccggatgc
     5101 cgggatagcc gtcgaccacc gactggatcg ctgccaaggt cttgtcgtag tcggccttcg
     5161 gatcgacgct gacccagttc tcgccgaagt ccaccgcgac cacctcgtcg gccaggaacg
     5221 cctgtccgat gtgcgcgccg aagttgcgca cgccggggat gtcgcgcaac tccttgctcg
     5281 ccgcgaccgt gacccgcatc tcttcgggca gggaggtgcc cggcgccgtg acccagtgca
     5341 tcaggaaatc tcgctccttg aacgacggca gcaggtcctg cccgaggaac ggcgtgacgc
     5401 ccaggcccag cgcgaccagg ccgccgacgg cggcataggc gggcctgggg gagcggacga
     5461 tgggggcgag agcgcgctca taaacgccgt gcacccaccg cacgaccggg gactcgcgct
     5521 tctccaggtc ggctttgcgg agcagcagca ggctcaacgc cggcgtggac accagcgcca
     5581 cgatcgtcga gatcaccacc gcaagcgcat acgacatggc gagcggccgg aagaacgagc
     5641 cggtgagccc ggtcaggaag aagaccggca gcagcgcgag cgcctcgatg atcgacgcgt
     5701 agacgacggc gccgcgaacc tcgagcgacg cgtggtagat gacgcgcgcg gtcgtgggca
     5761 gcgagcggtc gccgccggcg gcgcgatgaa tgcgcaatcg tcgcacgatg ttctcgacgt
     5821 cgacgatggc atcgtccacc accgccccca aggcgatcac gaagccggcc agcaccatcg
     5881 tgttgatcgt cgctccgcgc gcccacagga ccagtccggc ggcggtcagc gataggggga
     5941 tcgtcaccgc gctgatcagc gcgctgcgcc agtcgtagag gaacgcgccg agcacgatca
     6001 ccattagcag gacgccgagc agcagcgaac ggcccaggtt ctccaccgcg tgctcgacga
     6061 aggtggccgg acggaagatg gtcgtgtcga tctcgatgcc gggcagaccg ggcttcatct
     6121 cctcgatcgc ggcttcgacg ccgcgcgaga cctcgagcgt gttgccccac ggcagcttct
     6181 cgacgatcaa catcaacccc ggaccgtcgt tgatgacggc gtctccgatg agaggctggt
     6241 gtccttcggt gaggttagcg atgtccgcca ggcgcagggt tcgcccgtcg cgggatgcga
     6301 tcgggatctt cgccagctgg tccggcgtga ggatcggcag gatgtgacgc actccgatcc
     6361 gctggttggg ggtatcgacg aagccgcccc cgccgatgac gtggccgtcc gagaactgga
     6421 ggatgcccgc gtcgagcgcc tcggccgtca cctccatcac gcgctgcagc gaaacctttt
     6481 cggcccccag cttgccggga tcgacctcga cgtggagctg gctgacgcgc tcgccccaga
     6541 tcgtgacgtt cgccacgccg gggacgcgaa ggagccgcgc gcgcatcttc cagtacgcga
     6601 gcagcgacat gtcgatgagc gaatgatcga ccgatgacat gccgatcttc atgatccggc
     6661 tcgttgccga cagcggctgc atcatgaagg ggggagccga ccatgtcggg agcgtcgggg
     6721 tgacggaggc gagccgctct ccgaccacct gcctggcgtg aagcaggtcg gttcccgggt
     6781 tgaagatcag ctcgatcgag gacagctgct cgaccgactt cgaccggatc gcgtcgaggc
     6841 tcggcagccc ttgcagcgct tcctcgagcg ggacggtgac cagcgcctca acctcggcag
     6901 gggacaggcc gagcgcggcc gtttggatct cgacccgtgg cggcgcgaac tccggaaaca
     6961 cgtcgatcgg catctccttc agctgaccga ctccgaagac catcagggcc gctgccccgg
     7021 cgacgatcaa gaagcggaac cggaggctcg ttccgacgat ccaacgaagc atggcgcgct
     7081 cctttttcgt ctactggccg attccggctt cgatcccgtg cagctcggcg acgcccacca
     7141 ctacgaccgg cgttcccggc acgggaccgg cggtcaggat ggcgcgctca ccttcgatcg
     7201 cctccacgac cacgggtgcg cgggcgtagc cgccgtcgct ttcgacgaat acccatgtgt
     7261 cgccctcggc gtcgtagtag atggcgctgt agggaacgac ggtgcgtgag ccgctcgacg
     7321 gacgaacggg attggtctcg atgccgaggc gctcggcggc ccggccggtc agggtgatcc
     7381 gtgagacctc ggatccttcg atcgcctcga cgttcgccgg ggaccgcccg ccggtctgct
     7441 ccggcacgct ccggcaagcc gtgagcgcga gcagcaccac gacgggagct gcgatcagtc
     7501 gcttggcgtt catcctgtcc tcctacggct cgcgggtgtg gtgtccgtcg ccatcgggct
     7561 cgtgcttcgt catcaccgtt ccttcggccg ccggcggtgg ctgaacaggc acccgggcgg
     7621 attcagcggc gggctcgtcg agcacgatga cccgatgctc gccgccgcct tcgccgacga
     7681 agtaggtgcc ggtgtcgggc tcgagggccc ggccggccag caggtacagc cccggcatga
     7741 cgaacaaagt gagggcggcc gccacgaaca cgccgcccag caaaacgatc gccatcggcc
     7801 ggatgatctc aaagccttcg accggcgtga cgagcgtcag cggaagcgtg acgaccgcga
     7861 tcgccagcgt ggaggccgcg atgggaacgg cccggtcgat cgcgccccgc accgcgaggc
     7921 ggcggtcgaa cgcgacgccg tcgtagcgca atcggtgata gcgatcgagc aggccgagga
     7981 ggtggcgcag ggcgaggacg aacagcagca ggaaccccgc gatcgacccg agcgacacga
     8041 tgccgtcggt cagcaagacg gcaacggctc cgccggcgag cgatgcgccc gcgagcagga
     8101 tcgcaagaag ggccaggacc cagctgcgga ccgacgcttg ggcgatgagc agcgcgccga
     8161 tcgccgcggc gatggcgaag acgatgaact ctcgcttgcc cgcctgctgc gtgtcgaagt
     8221 cgcccatgat ctcggcgtga tgctcgagcg ggaacgcgat gccgtcgatg cggcgctcga
     8281 tgtcggcgag caccgctccg agccctcgtc cgcgaacgcc agccgtcacg tccatcgtgc
     8341 gcgacacggc ctcgtgccgg acgaccgtgg ggttgggtgc caggtgcacc gaggcgacgt
     8401 cgccgagccg gacggggcgt ccttcgggag tttcgatcag cagatcctgc aagctcgaga
     8461 tgttgtatcg agtctcgggc gagctccaca cgaccacgtc gaagaccttc tgctcctcga
     8521 acaggctccc gacgtggatt ccggccagga gcgtagcggc ggcgcgccgc acgtctcccg
     8581 gcttgatgcc gagcgtttcg gccgccgtca ggtcgacctc gacctcgacg gtcggctcgg
     8641 taggtaccgc cgacaccgcg gcatcttcga cgccctcgac gccggcgatg gcgctgcgga
     8701 cctcgtcggc tttctcgcgc aggacatcca ggtcggatcc gaagagccgc acggtgaccg
     8761 gatgctcgac accgctcagc tcgtcggtga cacgctcctt ggtataggtc ccgatgtcct
     8821 gatcgaagcc ggggtagccc gcgacgatcg aacggatgcg atcgatggtt tgccggtagg
     8881 gggccgaggg cgagaccgat acccacagtt cgccggcgtt gacgttgacg acgcggtccg
     8941 aggtgatggc gcggccgacc tggatcgcga ccgagtccac gccgtcgacg ccccggatct
     9001 cgcgagccat gcgctgggtg atgcgcgtca tctcgggcag cgaagtgccc ggggcggcct
     9061 cgaggtcgat cgcgaagtgc cgctcctgga tgccggggtc ggagctgtgc cgaaggaacg
     9121 gcaggacggc gaatccggca accgtcacta ccagcgcgac gatcagcggc gtgcgcgagc
     9181 ggaggaatcg cggcagcatc ctcgcgacca ggccgcgcgt gccgcgcgcc gcagccgtgt
     9241 cgggtttggc gctcgcgcgg ccgagcagcg ccagggcgag tgcgggcgcc accgtcaggg
     9301 ccacgatcat ggatgtgatc gcggcgagcg cgaacgtcca ggccaggctc ggaaggaacg
     9361 cgccggcgac gccgtcgaga aggaacagcg gcatcacggc gagcacgacg atcgcggttc
     9421 cgtaggcgag cgggctgcgg accgccagca cggtatcgtg gaccgctctc acacccagcc
     9481 cgccgggacg cgcctcgccc cggcgggtgc gccggacgac ggcgtcgacg ccggtgatgg
     9541 cgtcatcgat cacgagcgcg agccccatca ccagtcccgc accggtcatg gcgttgagcg
     9601 tcagcccggt caggcccatg atcgccgcgg tcgcgagcag cgacagcggc accgtgatcg
     9661 cgacgatgag cgcctctcgc catcccatga agaccaggaa cagcatcacg atcatcagga
     9721 cgaagccgat cagaagcgcg cggcccaccg tgctcgtcgc tcgttcgatc gaagcggcgg
     9781 ggcggaatag ggacgcatcg acggtgacgc ccttgagcgc gggccgcagc gtctcgaggg
     9841 cttcgtccag gtgacgggtg acttcgagga tgtccgcctc ggggaacttc tcgatcacga
     9901 ggagcaggcc agggccgccg gccagaagcg cgttgccgat gagcggctgg tgatcctcga
     9961 ccacggtcgc gacgtcaccg agccggaggg gctgcgggcc ggtgtcgtcg acggcgatct
    10021 gcgcgagatc ctcgggggtg cggaccggca gcagatgctg gatggcgagg cgctggttcg
    10081 gcgtgtcgat gaacccgccg gtgcccggcg tggatgcttc gaggaaggac aacggcgaga
    10141 cccacagcgc gttgcccgtc gtttcgatga tctgctgcag tgtcacctcg tgcgcgcgca
    10201 gccgttcggg atcgacgtgc acctgcagct gctggtcccg gttgccccac accgacacgt
    10261 tcgcgacgcc gggaacgccc agaagccgcg gcttgacggt ccaccgcgcg agcaccgaca
    10321 gctcgatcag gctcagctgc tcggacgaga ggccgatgat catcgcccgg ccggtggacg
    10381 agacgggttg gagcatctgc ggcggccttg atgcgccggg cagcgcgacc accgcctcgg
    10441 cgagacgttc ctgaacaacc tgccgtgcgc ggaagatgtc ggtgccgggc tcgaagacga
    10501 gctcgatcga ggacaggccc ggcatcgact ccgagcggat ggccgtgagg aacggcactc
    10561 cgttgagcag atcctgctcg aagggcgcgg tgatcagatc ctcgacctcg cgagccgaca
    10621 ggccgagcgc ctcggactgg atatgcacgg tcggctgcgt gaactcggga agggcgtccg
    10681 cgggcatcga gcgcagctgc tgcgcgccga ggaacatgac ccccgcggcg atcgcgatca
    10741 cgagcccgcg ggctctgagg ctcgacccta ccatccaccg aaacatggcc cctccctcgt
    10801 tttggcctac gtcagatccc acgagccgac gatgtctccg gccagtgccc gaaggcactc
    10861 ggtgtcttcg atcaagcggc cggtgagttc gcggcgcagc gcgtccggca tcgccgtctc
    10921 gcggtcccgc gccgccgggc gatgcgcgtt gtgccggatt gccccgcgca tcgcgtccac
    10981 gagctccttg gcttccttcg cggcagggaa ccgccgcttg atcgtcgtgc gtagatgcca
    11041 ggcgatcggc gagtggatcg tgcgtgcatc cgtgcggtag aactcctggg tgtgcaccgg
    11101 cgcgcgatag gatggatcga cgccgaggaa tcggtacacg gcttcgatca cggtgctgcg
    11161 gtcgttcttg agcgcctccg atgccatgat gagcatgcgt tcgcgcgggt agtgacgcag
    11221 gaagcgatgc gcctgcatgg cgtagcggct cgcgttgatg taggtcgggt cgctccggat
    11281 cgccacgttg atggggcggc gctcggaccc caccgcaacc cggtgacggt agtgcgacaa
    11341 gatccggtcc accgggtcgc gcacgacgta gacgatccgt gcgtgcggga tgaccgcggc
    11401 catgcgctcc ggcacgcccg tgaactgcgg gaacttcgaa tagttcgtcg aggcctcgcc
    11461 gaccgctttg gcgcgcgggg gaaccgcggc gaagcggcgc tcgtaccagc gaagcccccg
    11521 ttcccagttc gcctcggcga ggaagaagtc gagctctttc acccgcggca tgaagacctc
    11581 ggggtgcgcg cgcaagtagt ggtacaggct ggtggtcccg gtcttcatgg ccccgacgac
    11641 gacgaaattc ggcagcgtca tccggcttgg ctcctttgct cggccacctc ggccgctgtc
    11701 ccggtgaagt cggcgggctc gtagcgggag tcgggcaggg tgatgacacg ctgttgctcg
    11761 ggcttgcgcg cgcaaaagcg atgacgggcc gtgaggacgg tctgccacag cacgcgcacg
    11821 gtccggccga ccgtcgcgtt gcggcagaac tccaggtcga cggcgacccg gcgcgggacg
    11881 acgttctcca gatagaacgc ctcgatctcg tcggtggtgg ccgttgccgg cgggagcgtg
    11941 tcctcgtcgt tgaaagtgat tacgccggga ccggtcacgc ccggccggta tcggaagata
    12001 acgcgcagat ccggcgggta ttccgccgcg aagcccggcg tctgcgggcg aggtccgacc
    12061 agcgtcattt gtcccatcag cacgttgagc agttgcggaa gctcgtcgat gcaggtggca
    12121 cgaaggacgc gcccgagccg ggtgacacgg gtgtcaccgg gaacggtcag cgcgctcccg
    12181 gaggaatcgc gccgcatcgt gcggaacttc aggacgcgaa acggacggcc gccttgtccg
    12241 atacgcattt gtccgtacag tgccgggcca cggctcgtcg agcggatcgc gagcgcgacg
    12301 agcagcatcg gcgccagcag aagcgcgagc gcgacggtcg ccacgatcac gtcgaagacg
    12361 cggcgtgcga cgctcggtcc gatgccgcgg ccgatgtccg gccggacgtc ggagtgtgat
    12421 cgggcagcag ccatgaagcc ctccttcccg agcccagaag ccgcaccagc gatcggtagc
    12481 cggcgcgaat ggtgatgtcg tcgagcgtga ccgcctgctc ggccaggaac acggggaaga
    12541 acgaggcgtt gaacgacgcg tcgatcgcgt gcatggccaa acggtcgttg atccagcgcc
    12601 gcgcgagcga cgcgaaccag ccgccgctca taaacgcgta cgcgaagccg gcggcgttcg
    12661 cccaatgcgt gtacggcgag gcgatacgcg cgccgttgag gtagcgatga tgctccggca
    12721 cccggccgcg cacgcccatg ccgcgatcca ggtagaacgc gtaggagctc gggaacttgt
    12781 agatgtcgcg gaacgggggc actgcctcgc gcgcgagctc ccaatccacc acacccgaga
    12841 tccggccgcc ggtgacgagc aggttgccca tccagaagtc gccgtgcacg cccgagaggg
    12901 gcagccgctc gccccgcatg cgctccgcgg cggcgcgcgc gtaggagaac agcgccgctt
    12961 cctcagggct ccatccgatc tccgcgcggt aacgggcgat gggctcctcc acgaagcgct
    13021 cgatctcgcg gtcacccagg cgcgccgcgc gatccgcggt cgcccacgcg aacgagtcca
    13081 gccacgcacc ggcgcatgcg aagtccgctt cgacgcgccg ccgggacgac acgtggcccg
    13141 gctcgtagta cgtggtcgtc atgggccgcc cggtgaatgc ttcctcggcg agcgcgagcc
    13201 gtcctgcgac gcggccgatc gcatgcggtc gcggagcgaa ggcgccgtgc ggcccgtccg
    13261 gaccgaagcg cagcagcgcc tcgtgctcgg cccgcagccg cggctcggcg gcgacgtcgc
    13321 gcgcgacctt gacgacaacc gccggatcgc cggcctcgtc gaagagcatg agcgtcacct
    13381 tcggggccgt ccggtacatg ccggccacga tcccgcctcg gacgcgaggc acctgaggga
    13441 cgccggaagc gagccagtcc acgatcgcat cgatcatggg tgctcctttc gtgcgaccac
    13501 cgccaccgaa ggccacacgc gggcacgaag ccatgccggc gagacggagg ttcccacgcg
    13561 caggaagcgc aacgccatcc ggcgaaccgc ggcgcgcgtg cccgacgccc gggagtaggg
    13621 gaagaagaac atccgaagga agaagtccat ctcggcgcgg cgagctgcgg gaatcaggta
    13681 tgccggagtc acatgatcgg gcgcggcgag gaacacgcgg tgatcgcgca tgccggcgag
    13741 gtgcagcagc gtccggaccc ggccgagcga caagcccttg cgccgcagcg gacggttgga
    13801 gaacccgccg tagaaccagc ctcccggctc gaccgccgcc atcagcgtcc gcagcccatc
    13861 gaactccgac gggcccgcgt gaaataccgc atcaaggtcc ggcacggcga tgccgccgta
    13921 gcgcgttgcc gcgaccgcac ggagcgactc gtcgagcccc gatgcgatga tcggatccga
    13981 gaaggcatcc atgtgcgccg catcgccgac cagcagaacc ggccccgttg cgggcggaag
    14041 aacgtaccac cagttcatac ggcgcacgtc gcggatcgcg ctcatgtcgg cctcaccaga
    14101 ccgatcaggg cggcgacggg cgaccgtgac tcgtgccgga accgaatcat cgccgcaagg
    14161 gtcagcgtcc ctccgtatgc cagcgccccc gcgagcatcg gcacgacgag gggcgccgaa
    14221 cgcgttgcca tgaccgccgc ggcgagcgga acgcaggcgg tggccgggag cgcgattgcc
    14281 tgcatcatcc gcgaggagcc gatcgccggc cgaagctgca acgcgtagcc gacgagcaac
    14341 ccggtctcgg tggcgagcgt ggccgccgcg gacccgaggt aggaccagat cggaatgagc
    14401 gcgagattga gcgtgaggtt ggcgctcgcg gcgagcgcga tggccttggt tcgctgccgc
    14461 tgccggtcgc aggccgccag cgacaggctc agcgtgttgg acatcatgcg gatcgggacg
    14521 acgaggacca ggatctgaaa cgcgagcgtc gcgcgagcga acttcggtcc gtagagcgcg
    14581 tgcaggatgt cggccgccag gagcgcaccc ccgattgcga cgggaagccc gatcatcgcg
    14641 agcgaacggt tcgacgcgtc gcgataacgg gcgaagcgcg tgggatgcgc cggccaggcg
    14701 cggctcatcc ggggatacac cgcatggttg agcgatcggg ccagcacatt ccagtgcatc
    14761 accaggctgg tacacgcctg gtagagcccc accgctgccg gccccttcat cagaccgagc
    14821 agcacggcgt ccccgcgcat ctgcacgagc ttgagggtgt cggcggcggc gaacggtgtc
    14881 gccgacactg ccgaacgaac cgcgaagccg ccgtgccacg cgggcagcag gagcgtccgg
    14941 cagcggagcg acgccgtgat ccaggcgatc aacgccccga cgaatcgcga tgcgaggaag
    15001 gcgatcatgg ctccgacggg accgcctccg agtccgactg cgataacggc tccgacgagg
    15061 aacatggttt cctgcagcgc cgtggcgatc gtctcgagac gcatgcgttc ccacgcgacg
    15121 aacgcggcga actggatgcg tgcgatcccg tcgaagatca ttgcggtggt ggcgagcgtg
    15181 atggtcatct gcgattccgg cgaagcgccg aaggtgcgcg ctcccacggt gagggcgatc
    15241 gagaaagcga tcccgaagac gaaggtcatc gccatcccgc cttcgagcca cccgcgcacg
    15301 tgccccggcc gccgggcgac ttcgcgggac agcagcgcgg ggtagcccca gtaatacggc
    15361 agcagcgacg tcagcgtgag cgcgaactga aagacgccga gccgccccgg cccttcgacg
    15421 cgggcgagcg cgacgaccac gccgagcccg gcgaggccgc ggaaggtgaa gctcacgacc
    15481 tgccatgccg agtttccggc gatgcggcgc agacccagcc cccgtgggac gatgtccggc
    15541 tgcgggaagg tcacgtcgcg atcacccgct gggcgggccg cgcgccgttg ccgtgcgcgt
    15601 cggacagacg caccggccgc gcacgcagct cctcggtggg catgcccagc aggctccggt
    15661 aagtccgatc gaggatctcg cacatccgct gctgcgagaa cagtgaccgc acgacgttgc
    15721 ggccgtgaat ccccatgcgc cgcgcctcgg acggatgctc cagcatccac aggatgcatt
    15781 cggcgacgtg tgcgggatcg cccggacgcg acagcagccc ggtcgcaccg ggccggacca
    15841 gatcggcaac gccgttgacg gcggaggcca cgaccggccg gccgctcgcg agcgcctcgg
    15901 tcagcgcacg tccgagacct tcgtagagcg aaggcatcac gaacacgtcg aaggccgccg
    15961 cgatgcgatg tgcgtcgtcg cggaatccgg tgaacagcag gtcgatgccg agccgtgcgg
    16021 cctcggcgcg tgcttcggct tccatcgggc cgtccccgac catgacgaag cgggcgtccg
    16081 gccgcaccgc cgcgaccgcg gccgcggccc gcacgaagtc cttcggcgcc ttctggaacg
    16141 tgatccggcc gaccgtgccg atcagcggca cgtcctcgcc gatgccgatc tcgtcgcgaa
    16201 aggcgcggtc acgtccgcga gggatgtcgg ccagctcgac cgaggacggc gccacccgca
    16261 ccgtcccggg cggggcgagc cggttctcga ccgcgtcgcg tgcgacttgc ggcgccacgg
    16321 cgaggaaggc gtgcgtcgag cgccgcgcgg tccggtcgat cgcgaggtac acgcggcggc
    16381 gcagccggct catatgatcg tggaacggga acgcgtggaa ggtatggatc accaccggga
    16441 cgtggcaccg ccgcgcggcc atacgtccga tcaggccggc cttggcggta tgggtatgca
    16501 cgatcgagaa gcgctcgcgt ttgatcagac ggacgagcgc gcgataggcg agccagtcgt
    16561 cgagcggccg gatcgtctcg gccagccgcg gaatctcgac cgtgcgtatc cctgcgcggc
    16621 gcgccggctc gaagcattcg ccgccgggcc gggctgcgac ccagacctca tagcgggccg
    16681 gatccatgcc cgtcgccgag tgcagcgtgt tgccacccgc gcccgtggtg aacttggtga
    16741 tgacgtgcag caccttgatg cgtggggcgg ccgggcccgg cggcagggag gccgccggtg
    16801 tggcgggttc cggctcggag gcccagacgg agtccggcag gctcatgcga ccgccgcctc
    16861 ctgctcgcgc gcccaggccc acatcgcggc gaggccgtcg gtgagcgcga cgcgcggccg
    16921 gtatccgaat ccctcgcgag cgcgggtgac gtccgcgccg gtgtgcttga cgtcgccgcg
    16981 ggcggagcgt ctccggacga cggagatgtc gcggccggtg atcgtctcga tcgccgcgac
    17041 gacctcgttc atcgaatgtt gcgccccgcc accgatgttc gccaccccgc gccactcggt
    17101 gacggcgacg cgccgcatcg cgaatacgac gtccgatacg tcggtgaagt cgcgggtctg
    17161 ggtcccgtca ccggagagct cgaacgtccc cgcggttcgt gcggcgctca tcaggcgagc
    17221 gaacgccatg tcgggacgct ggcgcggacc gtagacggtg aacaggcgca gcgacgccgt
    17281 cgagatgccg aacgcgctgc ggtacagctc gcacaggtgc tcgcccgcca gcttggtcac
    17341 gccgtagggg gacagcggcc gggggagcgc ggactcggcg gtcgggtagg cgtcggcgtc
    17401 gccgtagacc gacgagctcg acgcgtagac gaacgagcgc agcggtgtcg agcgcgcggc
    17461 ctcgagcagc cgttgcgtgg tagtgatgtt ctgccgggtg tagtgatcga agtcccggcc
    17521 ccacgacgga cgcacgcccg gctggccggc gaggtgatag acggcatcga ttccggtcag
    17581 caggtcggtc agccgcgtgg cggcgacgtc ggcttccacg aaggtgaacc gcggatgagc
    17641 ggaggcgtct cgcacgttcg actccttggc ggcccggtcg taatagccgg tgaagcagtc
    17701 cacgccgcgt accaggtcgc ccgactcgag cagatgctcg accaggtgcg aaccgacgaa
    17761 gccggccgcg ccggttacca gcgcgttcac ggcgcgcctc ctttcgcgtc ctggtgggac
    17821 catcggtgga cggccgcgat caggcccaac agggcccaca gatgtcggta gtgcaggatc
    17881 tcgtgcgtga gcgccgacag cgcgagcgct acggcgacgc ccgtcatcgc cgcgccggtg
    17941 ccggcggcct ttcgccactc cggcttgacc acgcccgcat ggccgcgggc gatgcggtag
    18001 ccgatcgcgc ccgcgagcag cagcaacccg gcgaccccga tcggaccccg ctcgacgagc
    18061 gtggcgaggt agtcgttgtg cgaggagcgc acgccgctct cgccgctgcg gcctagctcg
    18121 tcccgtgtcg cgaacgggcc gatgccgagg atgccccgct cgcgatacag ggccatctgt
    18181 tccgagaaca ggtcctggcg attcgccgcg ctgcgttcga gccggccgat cgagtagcgg
    18241 atgaggaggt tctcggactc gggcgcgcgg cggaacgact cacccatcgc cacgtacgag
    18301 ccggcgccgg cgacgagaat cgcggaaagc accaccacgg ccacgatcgg gtcgattcgc
    18361 cgccaggcat acacgaacac cgcgacgacc gcaccggccg cgagccccag gatcgccgcg
    18421 ttcgatcccg tgagcagcat cgcggccagc atcacggcga cggccgcact gcggcgcggc
    18481 cacgacttcg gacgcgatgc gaccgcgacg aagatcgcga tcaggaagta gttgccggtg
    18541 atgttgggat tctggaagaa caggtgcgcg cgcgtgccgc gatcggtggt ggcgttgatg
    18601 agcggcgcga agccggaggc gacggccgcc acgaccagcg ctccccaaaa caccccagcc
    18661 gcgacccagg cggagcggat ctcttcgatc gcgcggggcg ttcgcgccac cgtcgcgatg
    18721 gccgcggtga acagcagcag atgggcctct tgcagcatga ccagcagcgt cggccccgcc
    18781 cggtcggcga ccaggccgct gatcgcgccg gccgtcatca gcacggccgt cgccccggcg
    18841 aacggcgcga agatcgcccg tccttcggcg gcgatccata cgaagaccgc gatcgtggcg
    18901 gtgaccatga aaaagtccgc cggcccggag tttcccggca ggcgcggctt gaccaggggg
    18961 atcacggcga tggcgaacat cacgatgcgc agcagggcgg cgggtggctc gccggttcgc
    19021 tgccagggca tgcgcggcac gcgatagacg gtcggcgcgg ccgggttcaa tgcagaaccc
    19081 ccaggggacg caggcgcgcg accgggcgcg cgatgtcgtg gcgggtcagg atcgacagcg
    19141 cttcgtccac gccccggtcg tcgtagtgac ccgccgcctg cgggccgcgg ccgtcatagc
    19201 gcatccgcag cgtggctctg ggccgcgggt cgggtcgcga caggccccgg ctgatgaagt
    19261 ccgcgacgat cccacccgag ccgtggcacg cggagtacag gctggcgtcc gcgccggacc
    19321 cggcgacgca cacgtaggac gaggtgcgcg cggtgccggg aagcagaagg gcctggccgg
    19381 tatgggcgaa cggacttccg ggctcgaatc gctgagccgg ccaggcccgg catgcattgt
    19441 ggcggtgcac gatcgcaccg tcctcttcgt agatcgagtt atgcggcgag tccacgacgg
    19501 ggcggactgc cggcgcgccg agcgtgcggc gcgcgagcgc ggccagcgat gccgcgaccg
    19561 cgccgcggaa cgcgaagccg aagttcatcg cggcggcgtg cgcgagcatc acgcgacggc
    19621 cttcgacgcc gtcgagcggg atcgcggcac cttcccggaa gtacagcgcg aaccgccggc
    19681 gcacctgttc gatcgagcgg gccgagcaca gatggtggcc gggcttttgc aacgccatcg
    19741 tcatgcgcac ggtcgtgggc gccttgcggc gccgcgcgta cagccggccg atctggccgg
    19801 ggagcacgcc cccgccgccg tggaactgga tcgtcagtcc gccttccgac aggcccagcg
    19861 cccgcgccgc gggcgggtcc aggacggctt cgacgcgctg gagctcgatg aaatggttgg
    19921 acgggcctac ggcggcgaag cgcatgcccg acagcacgcg cgtcagccag gggatctccc
    19981 tgcgcagcgc ggcggggccg ccgaagcggt ccaggtcgag tcggcccccc tcctcggtcc
    20041 gggcgcgcgc cgtcgcgggc agcccgtagc gatcaaccgc gaattcggtc cccagcgtcg
    20101 cggccgcgat cacgtccccc gtgccgagtg tgcggttcag cccgggcggc cacggatgag
    20161 cggctcgcgt ctcgctcaga aatcgaacca cgtcctcggg tcctgccgtg ccgccgtcga
    20221 gcgcgagcag cgtcatgccg cagttcagcg acgatgcggt gaacgccgga cggatcgtgc
    20281 ctcgggtggc gacggcgatg ctcgacggca tctccatgcg gggcttgtga agaaaatcgg
    20341 gaagcacgac cggcggcgcg gccaggtccg cgtcgcgcac gccccgcatg agcgcggcga
    20401 gcgcggcggc gtccgggggg ctttcgtctc cggcgaagat gcgcgggatg gatgtgtcgg
    20461 gccgggcgag ggtcatcgtg tgatcgccac ccggtcgcgc gcatcgggga cgctgcgccg
    20521 cgccgagtat ccgccgagcg catccgaaag gcggattccc ggacgcgcaa gggcgacccc
    20581 gacgcacggc agtctgtcga cgagtgcgcg cgcgaccggc aggaccggcg gaagcgccgg
    20641 cccgtatccg gtcgtcatcg cgagcgtcgc cgcaagcggc cagaagcggc gcagctcgcc
    20701 gcacgcgtag gtcgacgtca ccatcaggcg cgcaagctcg gccgggtcgg cctcacgcac
    20761 gcgtgtctcg cgtcctcgct cgagcagcac cacgacatcg ggccgcgccg acggcagccg
    20821 ggtctcgggc acgaccgaca tgcgccctcg cgtcgtcggc agcgcgatca cgtcgtcggg
    20881 gaccgcccac ggctcgatgc gaaacggctc ggcgacgccc cagacggtgc gggcgtcggc
    20941 gacgcacagg ttgtcggtga tcggaagcgc tccggcggcc atctcggcgg cgagcaccgt
    21001 ggaggtacct gcgcccggca tcccgacgac cagcgccgtg ccttgatcga tccggacgca
    21061 tgcggcgtga agaggagggc gggctgcggt cgatgcgcgc cacagtgccg gatactgggt
    21121 cagcatcgca ccgagccgga gccgccatcg ggccgggaag gcccggaaag cccgcagctc
    21181 gattcccggc cgccaccgga agatcagttc cggcggggaa tgccatgcgt ggacgagcag
    21241 gtcgaacccg gattgcgcgg cgttgcggat cacgacctcg cggtcccgcc gggccaacgc
    21301 gcggccggcc tcgtcccagt cgcccgggaa cggctttcga tcccgctcga ccacgacgcg
    21361 cagggtctcg tcgccggatc cgggggcgag cggcgatccg gtcgattcct cgacgaggtc
    21421 cgcgacccag ccgatcgcca cgtcgaaccg gatgcgctcg cccgccgtct cgatcgtccc
    21481 tggcattgcg ttccccgcgc tcagctcgcg ccgtcgccgt tgccggcttt gggcttgccg
    21541 ccggacatga atgcctcgga cgctctttcg aaggccggga cgtgccggcc gcgaaacccg
    21601 tcatcggatg ccggttcggc ctcgagtctc gggacggcct tgaacacgac ctcctgcggg
    21661 tggcggcgcc tgccgcgctt cttctgcacc agcacgaagc ccaggagcgg ccagccactc
    21721 gcggcggcga cgtgatggag cacggccagg gacttccggt gcagagcctc cggcgcgacg
    21781 gcgacgatgc cgccgtcgga gggggcggag cgatcgacga tcgcgatgcg cgatccgggc
    21841 accggaccgc ccgccggctc gctctccggc tcgccctccg gcttcaggga cgaggcgaga
    21901 tcgtcggcgg cgccggcctc cgtgccgacc ggaacgacca gcaccgaccg cacgtcggcg
    21961 cgtgtcgccg cgagccgtat ggccctggtt accgtcgctt cgcgctcgtc gaggtcttcg
    22021 ggatcaccgg ccggaacggt cccgagccac ggcgcgcccg ccgcggccgc gatggcctcc
    22081 gagcccatga ccgtcgggcg cagcacctcc gacgccgccg cgattgcgag cccgccgagc
    22141 aggcccgcga cgagcccgag catcaggcgc agcgcgtggg gcgtgccgct gggcgacgcc
    22201 ggcacgttcg ccgagtcgag caccgccgcg cgcggctgcg ccgcgccgat ctgcacgatc
    22261 tgccggcggt ccacgtccag ctcggcccgc tgacgcacgg ccgccgtgta ctgctgcaac
    22321 gcaacctggg ccgcctgcgt gcccggcgcc gcggcgcgcg ccgcggcctc gagcgtgacg
    22381 ttgcgctcgt cggcttcggc catgcggcgg tcgaggtcgt ccaggagcgt ttgcaccccg
    22441 ccacggagca tgttggatcg aacgtcgatc agcgcgagcg cgatcccgtt ggcgatcgag
    22501 gcggcggcat gcggatcctt gtccgacgcg gtgatgtcca cgacggagga cgagccggcc
    22561 gccgtcagca cgatcgaatc ggcgacctcg ccgacgtcac gctcctgatc gatcgtcgag
    22621 atcgcgcggc gtacgacgag gtcgctcgtc gcgatcgccc gcgccgaatc ggcgagcgtc
    22681 ttggccctgg ccccgtcgtt cggatcccat ccgccgagcg acacgcgcga cgtcgcctcg
    22741 tagcgcggct cctgcgttgc gccgatggcc aacgcgccga gcaatccgat gatcgtcaag
    22801 acgatcagga tcctcgcgtg gcgcacgaag atcctccgaa gagcttcatt ggtgtccatg
    22861 gatcgtcctc tcccggctct tgccgacgaa aagacgctag ggatgttgcc tttcgcgctc
    22921 cagagacgat tcgcgtaccg cctctgcgat gcgcgacgta gaacgcccgc acgcccaatc
    22981 ggggatacgt cgtcggcacc ttgcggccgg acgcgatgcg cggaacgatc gcatcgatga
    23041 gcacgcagcc ggttccgttc gtcgtcggcg cggcgcgttc gggaacgacg ctgctgcggg
    23101 cgatgctcga tgcccacccg gatctggccg tgccggggga gtcgcacttc atcgtgcgca
    23161 tgctcgcgac gcggcatgtg tacgagcagc cgtggggagt agatgtgcac ggctatcggc
    23221 gcgcgctcgc gcgcgaacgg tacttcgcgg catgggggct tccggcgccg gacctgcgcg
    23281 acgcgctggt gcgggccaac cctcgctcgc tcgaggatgc gatgcgggcc acgttccgag
    23341 cgtacgcgcg gcgtcagggc aagcagcgct ggggagacaa gacgccgtcc tatgtgctgc
    23401 acgtgcgcgc gatcgcgggc tcgtttccgg aggcgcgcat cctccacgtg atccgggacg
    23461 gccgggacgt ggcccgctcg atggtggacc tgggctgggc ggcctcgcac gaggacgcgg
    23521 tgctgcgctg ggcccatcgc gtgcgtcgcg gccgcaagga gggcgcgcgg ctgggcgagc
    23581 gatacgtcga ggttcgctac gaggacctcg tcggcgatcc cattggaacc ttgacccgcc
    23641 tgtgcgtctc gctcgatctg cctttcgacc ccgcgatgac gcagccgcac cgccgcgccg
    23701 acgagatctt ggcgacgctg gccaatcccg agaaccacca gcggatcagg cgcccgccct
    23761 cgcgcgtgcg cgactggcgc accgagatgg actccgagtc gatccgcaag atcgagtccg
    23821 tcgccgggga tcttctcacc gagctcgggt acgagctggg cgggccgccg gcgaccttgc
    23881 cgctccgggt ggccgcgcga agcgcctcgc ttcggagcct gccgcggcgg atgggcagcc
    23941 gctggcggcg aaccttgctg attcgctgaa ttgcccgcgg gctgccggcg ggtgccggta
    24001 gcatcgtcgc cgtgacgcct tccccgcttt gcgccgcgat gctcgccttc gccctggcga
    24061 cgctcgccgc ttgcacggat accgcggccg agtctttgct cgaccgggtc gccccggccg
    24121 catcgcccgg cgcacccgcc accgagccgc ccgcggccga gccgcctgcg accgggcccg
    24181 gccccgtcgc cgcctcaccg acgccggccg cgatcccggc cgaccccggc gtggtcatcc
    24241 gcgtcgtcga cggcgacacc gcgcacattc gcctggcgtc ggggaccatc gaaaaggtcc
    24301 gcttcatcgg cgtcaacacg cccgagagca cgacccgcca cgagccctac ggcaaggagg
    24361 cgtcgcgatt caccactacg cggctcgacg gccggaccgt ctatctggaa tacgacgtcg
    24421 agcggcgcga ccgctacggc cgcctgctgg cctacatatg gatgcgcccg ccgtcggacg
    24481 gctcgccctc cgacgtgcgc gcgcacatgt tcaacgccac cttgctcatc gagggctacg
    24541 cgcagacggc gacctatccg ccgaacgtgc gctacgccga cgtcttccgg gatctgcagc
    24601 gcgaggcgcg cgagcagggc cggggcttgt gggcgctccc ctccgaagat cagggggcgt
    24661 cgcgtttccc ggcatcgagc gcgtcctcgc cccgctcggg atgcgatccg tcgtaaggcc
    24721 gcagcggcgg gggagcggcg cccggctcta gcaggcggcg cgcaggatct tgtaggggtt
    24781 gcgggcggct ccgcccgccg gatggtactc gaagtgcaga tgcggtccgg tacagttgcc
    24841 ggtgcatccg acgcggccca ggatctggcc ggccgtgacc gactcgccgc ggctgacgta
    24901 ggtcgaggac aggtgggcgt accaccattc gttgccggcg gtgtcccgca ggatcacgcc
    24961 ccggcccatc cagccgcccc gcaccacgtc ggagacgcgt ccggtcagga ccgcgaccag
    25021 cggcgttccg gagttggccg gcatgtcgat gccttgatgc gggccgccgc gacggcggtc
    25081 accgaagtcg ttggacagcc cgtgcgggcc cgagaccggg caccggaagc cgcggatgct
    25141 gctgcgcgag gcgcgcgtat tgctcgacat cgccttggac aggtcgagca gggactgcca
    25201 ttcgcggagc ttggcgtcca gaccggaccg gcgcttctgc agcgtccgca gagccgccgc
    25261 ggccgcgtcg cgggcctggc gaagctcgac gctttcggtc ttggcgcggc gctggagcgc
    25321 gacgatgctc tcgagcgtgc cctcttcggc ggagcggatc cgctcgaggt agccgaggcg
    25381 ttcgacgaaa acgtccacat cctgagaggc gacgatcgtg tcgatgagcc ccatgctgcc
    25441 ggcgatgtac atctcggcgc tgcggcgaga gtgggcggtg cgaagggtcg cgagcttctt
    25501 gcccgcggtc gccagcgcac gcgtgtgcac gtcaacggcc ttctgggccg cgtccagctc
    25561 catctcggcg gccgacagcg acacggcgat cgcgtccaac gtcttcttga gcgtcgcgac
    25621 ccggctctcg agctcgctgc ggctgacggc cccggccggc agcgaaggca gcagcgagac
    25681 cgcgatcaag gcggcaagaa ctacggcgat gcgcttcgag atagctcccc tcccaatcct
    25741 tcgggcgact cttcgtgtgc cccggttccc cggtccggat acccgcccgg ttcccccggt
    25801 cccgttaccc gcccgagtac ccgaaggtag gcttcatcgg c
//
LOCUS       DATLFO010000257         5137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_5479, whole genome shotgun sequence.
ACCESSION   DATLFO010000257 DATLFO010000000
VERSION     DATLFO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5479"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2343
                     /locus_tag="VM841_10535"
     CDS             <1..2343
                     /locus_tag="VM841_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HVL90656.1"
                     /translation="DGMSARMVALAAAFAFGAVASSAIAPGAPASVAAAIVAVACLSA
                     GAVAARRAHALAWSAAILAAFALAFGLAGLRLASLERAALAAGGRDRADATLQGQVLG
                     RPAASGGSLRFAFGVAHAEIDGRPMRVRERVLVTIRPPPARWPRAGDLLTVDAGLAPV
                     RRPPTSPRRAEPEAAAALRLRNEGIAARAYARDVRVTGRSRNPLIAVAAAGQHAAEAA
                     IAALPPREGGLLLGVTVGDTVRLDPDTEANFRATGLSHLLAVSGGNLAMFLGAILAIL
                     GVVRARIGTRIAVLSVATLAFMAITRFEPSVLRAGAMTAIGIGGLAAGARARSLHTLA
                     AGCLGLMVWDPLLVHSGGFQLSALATFGLLVIAPRLRRAMGGGTIAAAAAVSLGAQLA
                     VGPLIALRFHETSLVALPANMLAVPAVGPATVIGFVASALATVAEPAGRVVALAAYPF
                     LWWMESVAATLARIPGASIRTPDGPAALIVVGASIGLIAGAMRMRRLRAAPLLIGVLI
                     VASFTVWSRAVAPVLPSGLRVIALDVGQGDATLVRAPNGATMLVDGGPDEALLIKRLR
                     AHGVRRIDVVVLTHPHTDHLAGLFAAIERLPIGRAIDAGEEATQDIAARWDEYPAALA
                     ARGVPRVIVRAPARFALGEATVDVLGPREMMRGSDADVNNNSIVLRVRYGSACALLGG
                     EVQEEGQDALLRDEPALRCGAFKVPHHGSARALREFYAATRARIALVSVGANRYGHPA
                     IAPMRWAAELGMRILRTDIHGDVTVTLGREGGFHVVTNPA"
     gene            2374..3426
                     /locus_tag="VM841_10540"
     CDS             2374..3426
                     /locus_tag="VM841_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HVL90657.1"
                     /translation="MKRIRVRSFFLGIMLARPRILWPLLILAAALVAPAAVRAADPCP
                     APACITISGTVRGPVFEAVSGYRVLVQRADGYNVTVVTDASGAYSATVPLPSSSSQCY
                     QIVGQADAFYANAATGAKRCSTGRVDLSPKVRIQGTLGEQKMYLPDASAPVALPVEVS
                     ALSRTYPAAFEGAPLPWVFKHSDPYQEASGGGYEHGHDGERGVFDAPSVRKIADEVWQ
                     YRWTRTIHLDHGAGFYEMDWGRGASMFDPMMECKMIWFGYGIDSLAPSSAVPGQTVTI
                     MGRKFGSTPGALVLAGSGQVTTISGSSIVSWSKTEITFVVPPLAKTGWISVLPPTNLP
                     TNAAYLNLDPVRIRLP"
     gene            complement(3451..3801)
                     /gene="mnhG"
                     /locus_tag="VM841_10545"
     CDS             complement(3451..3801)
                     /gene="mnhG"
                     /locus_tag="VM841_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HVL90658.1"
                     /translation="MKVAASIFVLTGASLMVIAGVGLLRLPDVFSRMHAQTKAATLGL
                     ACILTGTALSLPGPAVAKVVLALVFQLITAPVAAHMIGRAAYRAGVTMWEGTLYDELG
                     SHGRAAEEAPGPRE"
     gene            complement(3798..4055)
                     /locus_tag="VM841_10550"
     CDS             complement(3798..4055)
                     /locus_tag="VM841_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HVL90659.1"
                     /translation="MIAVDVGLVALGVSFLAGVTRAAIGPSTADRAVAADLCLFSVVG
                     ALALLAVRLDAVAFLDTVLVATIVGFIATVSLAYLIERRIK"
     gene            complement(4052..4393)
                     /locus_tag="VM841_10555"
     CDS             complement(4052..4393)
                     /locus_tag="VM841_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HVL90660.1"
                     /translation="MIVWRVFLRIIRVIRFVGFFLWELIAANAVVAWEVVTPSTFMRP
                     GIIAVPVRCRTEMEVTLLANLISLTPGTLTLEVSEDRSTLYVHGLHVKSPEHLRERVG
                     LLENHLLEVLR"
     gene            complement(4390..>5137)
                     /locus_tag="VM841_10560"
     CDS             complement(4390..>5137)
                     /locus_tag="VM841_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL90661.1"
                     /translation="LTKVGVYALYRVYSVVFDGDPAIRTLLLVIAATTMIVGVLGAIG
                     RERVTEILSFHIVSQIGYMIMGLALFDSMGLAGGIFYILHHIVVKTSLFLSAGALRFF
                     DDTDSLRDLGGVARERPVQSAAFMVAALSLAGLPPLSGFFAKLILLRAAFATGEYWIA
                     GVAIAVSFLTLASMVKIWNGVYWGERPGDAPERPLRRSRVAAVAAPAATLALISITIG
                     VAAEGMFTLSETAGGGLIDPTPYVEAVIGP"
BASE COUNT          717 a         1802 c         1853 g          765 t
ORIGIN      
        1 gacggtatga gcgcgcgcat ggtggcgctg gcggccgcgt tcgcgttcgg cgcggttgcc
       61 tcgagcgcga tcgcgccggg tgcgccggct tcggtcgccg cggcgatcgt cgcggtggcc
      121 tgcttgtcgg ccggggcggt cgcggcgcgg cgcgcgcatg cgcttgcatg gtcggccgcg
      181 atcctggccg ccttcgcgct ggcgttcggc ctggcgggtt tgcgcctggc ctcgctggag
      241 cgggccgccc tggccgcggg aggacgcgac cgggccgacg cgacgctgca aggacaggtg
      301 ctggggcggc ctgcggcctc gggcggatcg ctgcgcttcg ccttcggggt cgcccatgcc
      361 gagatcgacg ggcggccgat gcgggtgcga gagcgcgtgc tggtcacgat ccggccgccg
      421 ccggcgcggt ggccgcgcgc cggcgacctg ctgacggtgg acgcggggct cgcccccgtg
      481 cgccggcccc ctacctcgcc gcgacgcgcc gaaccggaag ccgccgccgc gctgcggctg
      541 cgcaacgaag gcatcgccgc ccgtgcctac gcgcgcgacg ttcgggtcac gggccgttcg
      601 cgcaacccgc tgatcgcggt cgccgccgcc ggccagcacg ccgcggaggc cgcgatcgcg
      661 gcgctgccgc cacgcgaagg cgggctgctg ctgggcgtga ccgtcggcga caccgtccgg
      721 ctcgatcccg acaccgaggc gaacttccgc gcgaccggcc tgtcgcatct gctggccgtc
      781 agcggcggca acctggcgat gttcctcggc gcgatcctgg cgatcctggg cgtggtgcgc
      841 gcccggatcg gcacgcgcat cgcggtgctg tcggtggcga cgctcgcgtt catggcgatc
      901 acgcgcttcg agcccagcgt gctgcgcgcc ggcgcgatga cggcgatcgg catcggcggg
      961 ctggccgccg gcgcgcgagc ccggtcgctg cacacgctcg cggccggctg cctgggactg
     1021 atggtctggg atcctctgct ggtgcacagc ggcggcttcc agctctcggc gctggcgacg
     1081 ttcgggctgt tggtgatcgc cccgcggctg cgccgcgcga tgggcggcgg cacgatcgcc
     1141 gccgccgcgg cggtctcgct cggcgcgcag ctcgccgtcg ggccgctcat cgcgctgcgc
     1201 ttccacgaaa cgtcgctcgt cgcgctcccg gcgaacatgc tcgccgtgcc ggccgtgggg
     1261 ccggcgacgg tgatcggctt cgtggcgtcg gcgctcgcga cggtcgccga gcccgcgggc
     1321 cgcgtcgtcg cgctcgcggc ctatccattc ctgtggtgga tggagtcggt cgcggcgacg
     1381 ctcgcgcgga ttccgggcgc ctccatccgc acgcccgacg gcccggcggc gctgatcgtg
     1441 gtgggagcgt cgatcgggct gatcgccggc gcgatgcgga tgcgccggct gcgtgcggct
     1501 ccgctgctga tcggcgtgct gatcgtcgcc tcgttcaccg tctggtcgcg cgccgtcgcg
     1561 ccggtcctgc cctcggggct tcgcgtgatc gcgctcgacg tcggtcaggg cgatgcgacg
     1621 ctggtgcgcg caccgaacgg cgccacgatg ctggtcgatg gcggtcccga cgaggcgctg
     1681 ctgatcaagc gcctgcgggc ccacggggtg cgccgcatcg acgtcgtggt gctgacccat
     1741 ccgcacaccg atcatctcgc cggcctgttc gccgcgatcg agcgtcttcc catcggccgg
     1801 gcgatcgacg ccggcgagga agcgactcag gacatcgccg cgcggtggga cgagtatccg
     1861 gcggcactcg ccgcccgcgg cgtgccgcgc gtgatcgtgc gggcacccgc gcggttcgcg
     1921 ctgggcgagg cgacggtgga cgtgctcggt ccgcgcgaga tgatgcgcgg cagcgacgcc
     1981 gacgtgaaca acaactcgat cgtgctgcgt gtccgctacg gtagcgcgtg cgcgttgctg
     2041 ggcggcgagg tgcaagagga aggacaagat gccctgctgc gcgacgagcc cgcgcttcgg
     2101 tgcggcgcgt tcaaggttcc ccatcacggc tcggctcgcg cgctgcgcga gttctacgcc
     2161 gccacgcgcg cgcgaattgc gctcgtcagc gtcggcgcca accgctacgg ccatcccgcg
     2221 atcgcgccga tgcgctgggc cgccgagctg ggaatgcgca tcctgcgaac cgacatccac
     2281 ggcgacgtga ccgtcacgct cggacgcgaa ggaggcttcc acgtcgtgac gaatcctgcg
     2341 tgaccgggcc gaatcggacg acttttggcg cgggtgaaac ggattcgcgt tcgctcgttt
     2401 ttcttgggca tcatgcttgc ccgcccgcga atcctttggc cgcttctcat cctcgccgcg
     2461 gcgcttgtcg cgccggcggc cgtccgcgcc gccgacccgt gtcccgcgcc tgcctgcatc
     2521 accatctcgg gaaccgtgcg cggtccggtg ttcgaggcgg tgtccggcta tcgcgtcctg
     2581 gtgcagcgcg ccgacggcta caacgtcacc gtcgtgaccg acgcgagcgg cgcctatagc
     2641 gcgaccgtgc cgctgccgtc ctcgtcgtcg cagtgctatc agatcgtcgg gcaggccgac
     2701 gcgttctacg cgaacgcggc gaccggcgcc aagaggtgct cgaccgggcg tgtggacctg
     2761 tcgccgaagg tgcgcatcca gggcacgctc ggcgaacaga agatgtatct gcccgacgcg
     2821 tcggcgccgg ttgcgctgcc cgtcgaggtc tcggcgctgt cgcgcacgta tccggcggcg
     2881 ttcgagggcg cgcccctgcc ctgggtcttc aagcacagcg acccttacca ggaggcttcc
     2941 ggcggcgggt acgagcacgg ccatgacggg gagcgcggcg tcttcgacgc gccgtcggtc
     3001 cgcaagatcg ccgacgaggt ctggcagtac cggtggacgc gcacgatcca cctcgatcac
     3061 ggtgccggct tctacgagat ggattgggga cgcggcgcga gcatgttcga cccgatgatg
     3121 gaatgcaaga tgatctggtt cggctacggg atcgactcgc tcgcaccgtc gagcgccgtt
     3181 cccgggcaga cggtcacgat catggggcgc aagttcggat ccacgccggg cgcgctggtg
     3241 ctcgccggca gcgggcaggt caccacgatc agcggctcgt cgatcgtgtc gtggtccaag
     3301 accgagatca ccttcgtcgt tccgccgctg gccaaaaccg gctggatcag cgtcctgccg
     3361 ccgacgaacc ttccgacgaa cgcggcgtac ctgaacctcg atcccgttcg catccgcctt
     3421 ccgtaattcg gttcccggcc cgcgcgacgc tcattcccgg gggccgggag cctcctcggc
     3481 ggcccgtccg tgggagccga gctcgtcgta gagcgttcct tcccacatcg tcactccggc
     3541 gcggtaggcg gcgcggccga tcatgtgtgc ggcgacgggc gcggtgatga gttggaacac
     3601 gagcgcgagc acgaccttgg cgaccgcggg gccgggcagc gacagcgcgg ttccggtcag
     3661 gatgcacgcc agccccagcg tggccgcctt ggtctgcgca tgcatccgcg agaacacgtc
     3721 gggcagccgg agaagcccga ccccggcgat caccatgagc gaggcgccgg tcagcacgaa
     3781 gatcgaggca gccaccttca cttgattctc cgttcgatca ggtacgccag cgacaccgtt
     3841 gcgatgaagc ccacgatggt ggcgaccagc acggtgtcca ggaacgcgac ggcgtcgagc
     3901 cgcacggcga gcagcgcgag cgcgccgacg acgctgaaca ggcacaggtc ggcggcgacg
     3961 gctcggtccg cggtcgatgg cccgatggcc gcccgcgtga cgccggcgag aaacgacacg
     4021 cccagggcga ccagcccgac atcgacggcg atcatcggag cacctccagc aaatggtttt
     4081 cgagcaggcc cacacgctcg cgcaagtgtt cgggtgactt cacgtgcagc ccgtgcacgt
     4141 acagcgtcga acgatcttcc gacacctcca gcgtcagcgt tcccggggtg agcgagatca
     4201 ggttcgcgag cagggtgacc tccatctcgg tgcggcaccg tacgggcacg gcgatgatgc
     4261 cgggccgcat gaatgtgctg ggagtgacga cctcccacgc gacgaccgcg ttggcggcga
     4321 tgagctccca caggaagaag ccaacgaaac ggatcacgcg aatgatccgc aggaagacgc
     4381 gccacacgat cacggcccga tcaccgcctc cacgtagggg gtcggatcga tcaggccgcc
     4441 gccggccgtt tcggacaagg tgaacatccc ttcggccgcg acgccgatgg tgatcgagat
     4501 gagcgcgagc gtggcggccg gcgccgcgac ggctgcgacg cggcttctgc ggagcggccg
     4561 ctccggcgcg tcgcccggcc gctcgcccca gtacacgccg ttccagatct tgaccatcga
     4621 tgcgagcgtc aagaagctca cggcgatcgc gacgccggcg atccagtact cgccggtggc
     4681 gaacgcggcg cgcagcagga tcagcttggc gaagaagccc gacagcggcg gcagcccggc
     4741 gagtgacagc gccgccacca tgaatgcggc ggattgcacg ggccgctccc gcgccacccc
     4801 gcccaggtcg cggagcgaat cggtgtcgtc gaagaatcgc agcgccccgg cggacaggaa
     4861 cagtgacgtc ttcacaacga tgtgatgcag gatgtagaag atgccaccgg ccaggcccat
     4921 cgagtcgaac agcgcgagcc ccatgatcat gtagccgatc tggctcacga tatggaacga
     4981 caggatctcg gtgacgcgct cccgtccgat cgcgccgagc acgccgacga tcatggtggt
     5041 cgcggcgatc acgagcagca gcgtgcgaat cgccggatcg ccgtcgaaga cgaccgagta
     5101 cacccgatag agcgcgtaga cgccgacctt ggtgagc
//
LOCUS       DATLFO010000258         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_26280, whole genome shotgun sequence.
ACCESSION   DATLFO010000258 DATLFO010000000
VERSION     DATLFO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26280"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VM841_10565"
     CDS             complement(<1..744)
                     /locus_tag="VM841_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVL90662.1"
                     /translation="MLSAFRNAFKIPDLRNKILFTLVVIGIYRLGSHLVLPGVDFEQV
                     QALNDPDRAADGIASLINLFSGGALTNFAVFALGIMPYITSSIIMQLLAVVIPRLEQL
                     QKEGEIGRKKITQYTRYMTVVLSLLQSLGLVVTANSGSLFGGGAGQPLGEFFPERTLT
                     TTMLVVLTLTAGTALIMWLGELITQRGIGNGMSILIFASIISTLPAEATSLWVTAGVA
                     KGLVFLSLGIAIVVAVVLVEQAQRRIPVQY"
     gene            complement(756..1196)
                     /gene="rplO"
                     /locus_tag="VM841_10570"
     CDS             complement(756..1196)
                     /gene="rplO"
                     /locus_tag="VM841_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVL90663.1"
                     /translation="MKLHHLRPAPGARTRKHRKGRGEAAGKGKTSGRGQKGTGARGTV
                     PIWFEGGQMPLHRRLPKLGGFSNAKFKTEYIPVNIESLNKFEAGTTVDPTLLRDAGIV
                     SKSRGKVKILGRGEITVALTVKAHAFSESAKAKIASAGGTAEVL"
     gene            complement(1193..1375)
                     /gene="rpmD"
                     /locus_tag="VM841_10575"
     CDS             complement(1193..1375)
                     /gene="rpmD"
                     /locus_tag="VM841_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HVL90664.1"
                     /translation="MKLKITLIRGLAGCTKRQRETVATLGLRRIRDVVERDDSPTVRG
                     ALDKVAHLVTVEEVSA"
     gene            complement(1372..2073)
                     /gene="rpsE"
                     /locus_tag="VM841_10580"
     CDS             complement(1372..2073)
                     /gene="rpsE"
                     /locus_tag="VM841_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HVL90665.1"
                     /translation="MVQGAAGGQGGGGQGGGGGRGQGRGGPGGGRGQGRGGPGGGRGG
                     PGGGRRDRDRDLDPGLPQLEQRVLPINRVSKVVKGGRRFSFAAMVVVGDRKGKVGIGY
                     GKAKEVPMAIQKAEEIARRSMFSVPMVGSSIPHEVLGQDGAGKVLLKPAAPGTGIIAG
                     GAVRAVMECAGIQDVLSKSLGSGNRLNIVHATIAGLRALRSPDDVAAARGRSLSEVTP
                     AAMLETYKNSKVKLS"
BASE COUNT          327 a          770 c          687 g          327 t
ORIGIN      
        1 gtattgcaca gggatccggc gttgggcctg ttcgacgagc accaccgcca cgacgatcgc
       61 gatcccgagg ctgaggaaca ccagcccctt cgccacaccc gcggtgaccc acaggctcgt
      121 cgcctctgcc gggagcgtgg agatgatcga cgcgaagatc aggatggaca tcccgttgcc
      181 gatgccgcgc tgcgtgatga gctcgccgag ccacatgatg agcgcggtcc ctgcggtgag
      241 cgtcagcacc acgagcatgg tggtcgtgag cgtgcgctcg gggaagaact cgccgagcgg
      301 ctggccggcg ccgccgccga acagcgagcc ggagttcgcg gtcaccacga ggccgagcga
      361 ctgcaggagc gagagcacga cggtcatgta ccgcgtgtac tgcgtgatct tcttgcgccc
      421 gatctcccct tccttctgga gctgttcgag gcgcgggatg accacggcca ggagctgcat
      481 gatgatcgag gaggtgatgt acggcatgat gccgagcgcg aacacggcga agttcgtgag
      541 ggcgccgccg ctgaacaggt tgatcagcga cgcgatgccg tccgctgcgc ggtccggatc
      601 gttgagtgcc tggacctgct cgaaatccac gcccggcagg accaggtggg accccagccg
      661 gtagatcccg atcacgacga gcgtgaacag gatcttgttg cgcagatcgg ggatcttgaa
      721 cgcgttgcgg aaggcgctga gcactccgac ccctcctaca ggacctcggc ggtccccccg
      781 gcggatgcga tcttggcttt cgcgctctcg ctgaaggcgt gcgccttcac cgtgagggcg
      841 accgtgatct caccccggcc gaggatcttc accttgcccc ggctcttcga cacgatcccg
      901 gcgtcccgca gcagcgtggg gtcgacggtg gtcccggctt cgaacttgtt cagcgactcg
      961 atgttcaccg ggatgtactc cgtcttgaac ttcgcgttcg agaacccacc gagcttcggg
     1021 agccgacggt gcagcggcat ctgaccgccc tcgaaccata tcggaacggt cccgcgagcg
     1081 ccggtcccct tctgtccgcg acccgacgtc ttgcccttgc cggccgcttc gccacgaccc
     1141 ttccggtgct tgcgcgtgcg cgcgccgggc gccgggcgga ggtgatgcag cttcatgcgg
     1201 acacctcctc gacggtgacc aggtgggcca ccttgtccag cgcgccgcgc accgtcggcg
     1261 agtcgtcgcg ctcgacaacg tcgcggatac gacggagtcc gagcgtcgcg accgtctcgc
     1321 gctggcgctt cgtgcacccg gcgagaccgc ggatcaacgt gatcttcaac ttcacgacag
     1381 cttcaccttg ctgttcttgt acgtctcgag catggccgcc ggcgtgacct cgctgagcga
     1441 acgaccccgc gccgcggcga catcgtcggg cgagcgcagc gcgcgcaagc cagcgatggt
     1501 cgcgtgcacg atgttcaggc ggttgcccga ccccagcgac ttactgagca cgtcctggat
     1561 gcccgcgcac tccatcaccg cgcgcaccgc acccccggcg atgatcccgg tcccgggcgc
     1621 ggccggcttg agcaggacct tgcccgctcc gtcctgaccg agcacctcgt gcgggatcga
     1681 cgagccgacc atgggaaccg agaacatcga acggcgcgcg atctcctcgg ccttctggat
     1741 cgccatcggg acctctttgg ccttcccgta gccgatcccg accttgccct tgcggtcccc
     1801 gaccacgacc atcgccgcga acgagaacct gcgaccgccc ttcacgacct tggacacgcg
     1861 gttgatcggc aggacgcgct gctcgagctg cgggagcccc gggtcgaggt cccgatcgcg
     1921 gtcgcgccgc ccgccgccgg ggccaccacg gccgccgccg gggccgccac gaccctgacc
     1981 acggccgccg cccggaccgc cacgaccctg accacggccg ccgccgccgc cctggccgcc
     2041 gccgccctgg ccgcccgccg cgccctgcac catcacgacg gcgacgacca cgatggccac
     2101 gacggggagg g
//
LOCUS       DATLFO010000259        11945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_144041, whole genome shotgun sequence.
ACCESSION   DATLFO010000259 DATLFO010000000
VERSION     DATLFO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144041"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="VM841_10585"
     CDS             <1..1903
                     /locus_tag="VM841_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90666.1"
                     /translation="GATTGSEQNHDIVYAIVQDAALLNGGLPSIDAPEGVAPGVRNTT
                     LNGIYVSGDFGSTWTLMADGSEIANNPASGSGLAGLGQALLSGPGVQAWYNEWILPDP
                     TRQDANGVPTRLLFGLEEVWQNELTSQPQNGRSSFKVIGRYYAGNTCMLLNTGLPFCP
                     TGRPLASETTTHPDQHDAIYVPDGKGGVTLIVGNDGGAYKQHVAEAEEFDQTKWGIGI
                     NRGFNTLLPYHAQAAKDGRVWYGLQDNGSGYIDGTTREQFMTFGADGGVVAVDPDNSD
                     YAWTESQFAGMRVTTDGGRTWTSAPPGITNYQFINPFVMDPTDSNHVMTGGRQVVETV
                     DGPRTCTAVQAAGESETIECSWVKVYDLGTHAKPGDASASATGSDPANSTTSVALQGA
                     NAYVGFCGPCDIINTTVPYKRGLATNVGGDAPAAKASPDGWHIASAKGLPNRTITSIA
                     IDPKSPKTIYVTLGGYSGRTWRGPGTFADKNKNRGTGSVFVSTDAGETFKNISGNLPN
                     APANAIVIRGSQLIVGTDIGVFMSKDLSGSAWAALANGLPVVPVVSVQVMPGETDRIM
                     IATFGRGVYTYKIPSALSPPPPPPAQVKGVKKELAATGVDDWFAAGVILAAMAAFLTT
                     RLRRGRPQA"
     gene            1964..2260
                     /gene="gatC"
                     /locus_tag="VM841_10590"
     CDS             1964..2260
                     /gene="gatC"
                     /locus_tag="VM841_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVL90667.1"
                     /translation="MAISDEQVLHVARLARLTLTDEEVGKFARQLSSILEHAERVMAL
                     AAEDVPPTSTAIPLVNVLRPDVAGECLPQDKALSTAPESEQGRFKVPRIMEEPQ"
     gene            2257..3720
                     /gene="gatA"
                     /locus_tag="VM841_10595"
     CDS             2257..3720
                     /gene="gatA"
                     /locus_tag="VM841_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HVL90668.1"
                     /translation="MSDLWRLDGATLAAMIAGGEVSAAEVADAHLARIEATDPDVHAM
                     LTVTAEHARAQAERVDDARAAGEPLGPLAGVPLALKDVLCTDGIRTTAGSKILGEYVP
                     PYDATVVQRMNDAGIVTVGKANMDEFAMGSSTENSAFGPSRNPWDLARVPGGSSGGSA
                     AAVAAGQAPLGIGSDTGGSIRQPAALCGIVGVKPTYGLVSRYGLIAFASSLDQVGPFA
                     RSVRDAALLLQSIAGHDPRDSTSVPGDAPDMVSGLDDGVNGLRIGLWTESMGEGTQQG
                     VRDRVREAADRLAGLGASVDEISLPSFEYALSAYYLIAPAECSSNLARYDGVRYGMRA
                     GGRDVAEMNSRTRAEGFGDEVKRRVMLGTYALSAGYYDAYYGKAQKVRTLVIREFEKA
                     YERFDLILSPTSPTTAFKIGERADDPISMYLADLFTIPSNLAGACGISVPCGLAEDGL
                     PVGLQLMGPPMAEPLMFRAANAFEADRGPLPDPPLWA"
     gene            3711..4103
                     /locus_tag="VM841_10600"
     CDS             3711..4103
                     /locus_tag="VM841_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90669.1"
                     /translation="MGVTAVDAQSNGAPQWSGRVPKPAAILMLQIVGGFFLIAGGLAF
                     WLGSALDWEGLAKAYAIIGGYLALTGPFALAAGWSRPPGLVAGMVALALMLAAIPIGP
                     ILVAVIGFLGIRNREHVRDYYRQRIPKR"
     gene            4100..5542
                     /gene="gatB"
                     /locus_tag="VM841_10605"
     CDS             4100..5542
                     /gene="gatB"
                     /locus_tag="VM841_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVL90670.1"
                     /translation="MSVSDYEPVIGLEVHCELSTNSKMFCSCPAHFGAEPNTNVCPVC
                     LGEPGSLPVVNERAVEDAIKIGLALNCSVAPRTIFHRKNYFYPDMPKNYQISQYDNPI
                     CTGGYLDVDGGRVGITRVHMEEDTGKSLHVGSSGRISEATHSLVDYNRAGVPLMEIVS
                     EPDVRSAEQAMTYLTELRSILATLGVSDVRMEEGSLRCDANISIHKPGTPWGTRVEVK
                     NLNSIRSLGRAVAFEIERQAKLLDAGEPIVQETRHFDESTGVTKTLRSKEEAFDYRYF
                     PEPDLVPIEPDEAWVAGLRAALPELPAARRVRLETEHGLATEHAAFVGASPASMSYFE
                     AVIAGGTPARDAAVWMAGELARWLGDRGADIADSPVSPKALAGLIKLVGEGAINLNTG
                     KRVLQLSFESGKDPATIVAEQGLEQVSDTGAIDAIIAEVLTANAAEVERFRGGEEKVF
                     GFLVGQSMKALKGQGNPKLVNERLRAALEG"
     gene            5546..6241
                     /locus_tag="VM841_10610"
     CDS             5546..6241
                     /locus_tag="VM841_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90671.1"
                     /translation="MASNAEQLLAQSVRNRPDTDYRFAWGTYIGWTILTMGIYSHYAT
                     YRLVQRRAEHARRRLAFSSYLWQVLAERADAADKRDLVQPGLDNLSRVHAEIESFERR
                     NKREPGLWTILRVVFTVVGAYINHFLNKDFRFLETWEVALAENAEWVMQRLEIGGPIA
                     KPAHRTPDRSTWLYVLLTVLTAGLFSIWWRSVIMKDGNEHFDAEERSEDAILAALGIA
                     PQTMTPAPPQPPL"
     gene            complement(6243..6581)
                     /locus_tag="VM841_10615"
     CDS             complement(6243..6581)
                     /locus_tag="VM841_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HVL90672.1"
                     /translation="MRAACPRCGAPADPDATACAACGYVRAPIAQARRPMPVPVEDRA
                     PAAMVLAVLGLAIPFVCSIAAIVLGLRSKRALSRSPDRGGRRLAAIGIWLGAFGLASW
                     TAIATLIALG"
     gene            6649..7473
                     /locus_tag="VM841_10620"
     CDS             6649..7473
                     /locus_tag="VM841_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90673.1"
                     /translation="MSMSPEQFLLAEIGRRDATDYRFRWGTYFGWAVLTGGLYSIYGT
                     LKLVERRVDHVRRRLAVSTSLWHALAAKADSLRVRGDVQEGLDNLSRIHAQIETYERK
                     NRRNAQTTAAVRAVLLFATFAGIAMFSALYDPDLGVAGQPDLVAVGSLIFDAGVIGLL
                     VHGAITNAALHRDFNFLDTWETSWAENVEWVMYRLGMAPSFPARPPQPTRSTATYVLL
                     TVFSLGVFSILWRASLMGDGNRHFDHDDEVEDAILAAMDLRGSPAPQTGLPVLPPS"
     gene            7515..9185
                     /gene="ilvD"
                     /locus_tag="VM841_10625"
     CDS             7515..9185
                     /gene="ilvD"
                     /locus_tag="VM841_10625"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVL90674.1"
                     /translation="MSDPQFDPRHRSRDVTEGPQRAPARAMLRAVGFTEEDFGKPQVG
                     VASSWNEVTPCNVHLNTLAGYAKEGVRAAGGVPVEYTTIAVSDGIAMGHEGMRASLIS
                     REVIADSVELVAHAERFDGLVTIAGCDKSLPGMVMGAARLDIPSVFLYGGSIMPGHYA
                     GRDVTIQDVFEAVGTHSAGNMTDDELLALERAACPGAGSCGGMFTANTMAAAIEAMGL
                     SLPGSASPPALDARRDEFARASGEALLPMLEQGLTPRRIITRASLENAITVVVACGGS
                     TNAVLHLLAIAREAETELELDDFDRIARRTPIIVDAKPWGRFVMLDIDKAGGIPAVMR
                     ELLDADLLNGDAITVTGKSIEENLRDTALSSDRSVVYPVAQPISPYGGFAILRGDLAP
                     DGCVAKVAGGHGSFSGPARVFDREEDAMAAVIQGRINAGDVIVIRYEGPRGGPGMREM
                     LAVTAAVNGAGLGEHVCLITDGRFSGATHGNMIAHVSPEAAVGGPIALVAEGDAITVD
                     VPNRRIDVAADLEARRGAWRPPAPRYTKGVFGKYATRVSSAAEGAVWR"
     gene            9239..9652
                     /locus_tag="VM841_10630"
     CDS             9239..9652
                     /locus_tag="VM841_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90675.1"
                     /translation="MKARAIAAAIVTASLVAPAAATDHLPYPAYSSCTERFIEGPISS
                     SCPFEYGGRRVTVFGAGTAWGEPMSITLEIVLREGPDEQVLFVCSAPATGAGPLSAPA
                     AQCVRRSPVLQVAPGTMLRCRHRGPGTGVFGCSSP"
     gene            complement(9656..10360)
                     /locus_tag="VM841_10635"
     CDS             complement(9656..10360)
                     /locus_tag="VM841_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HVL90676.1"
                     /translation="MVGSGEFTPAMTGIDRELLRSIGPSPRVVVLPTAAGGEDPGDWA
                     YRGVEHFTRLGAQSVALMVLDRIHASDPAHVAEIERADLVYFSGGKPARLMAAIEASP
                     LFDGMLRARANGAWIVGASAGAMVLGDWTLVHTEADPHGTPTIWTIGLGMLQGIATVP
                     HYDIWLEGPQLAAEISPQCTVFGVDEDSALLLDETEALVRGRGGVVVWSESGARRYAD
                     GARFSLTDAVRSLRRS"
     gene            complement(10442..10828)
                     /locus_tag="VM841_10640"
     CDS             complement(10442..10828)
                     /locus_tag="VM841_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90677.1"
                     /translation="MIARAIMAAALVAAALTGGAIAQEMPRGEIRCIVSEANPACGFF
                     AAGPAAFDSARVGPWVVRIYRDSELVWSYGGSAPSTWTIPSQPGDLVEAAIVTSDPLC
                     PPADGVPECHGAGVIVLTEKPAPETE"
     gene            11176..11823
                     /locus_tag="VM841_10645"
     CDS             11176..11823
                     /locus_tag="VM841_10645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVL90678.1"
                     /translation="MNRTALIIIDMQNDFAHPDGRAFLPDAAHKVPVMASVLDAFRQA
                     KAPVIHVVRTYRRDTWDVERFRVPYFQNQPGFLVEGTWGAQIVDRLTPEPGEPVVMKS
                     RFSAFAFTNLDLLLRRAGVNKIVVVGENLPNCPRTTMFDAIGLDYDVVAVPDALATTG
                     DETHHYNLQDLAAVGVRVAPSQDVIAEILREGDRYADATHGAMAARRDEERSEGR"
BASE COUNT         1895 a         4162 c         4119 g         1769 t
ORIGIN      
        1 gggcgccacg acgggctccg agcagaacca cgacatcgtg tatgcgatcg tgcaggacgc
       61 tgcgctcctc aacggcggcc tgcccagcat cgacgctccc gagggcgtcg cgccgggcgt
      121 gcgaaacacc acgctcaacg gcatctacgt ctcgggcgac ttcggcagca cctggacgct
      181 gatggccgac ggctccgaga tcgccaacaa cccggcgtcg ggatcggggc tggcgggcct
      241 cggacaggcg ctgctgtccg gccccggcgt gcaggcctgg tacaacgaat ggatcctgcc
      301 ggacccgacg cgtcaggacg ccaacggcgt tccgacgcgt ctgctcttcg ggctcgagga
      361 ggtctggcag aacgagctca ccagccagcc gcagaacggc cggtcctcat tcaaggtgat
      421 cggacgctac tacgccggaa acacctgcat gctgctcaac accggcttgc cgttctgccc
      481 gacggggcgg ccgctggcat ccgagaccac cacgcatccc gatcagcacg acgcgatcta
      541 cgtgcccgac ggcaagggag gcgtgacgct gatcgtgggc aacgacggcg gcgcctataa
      601 gcagcacgtc gccgaggccg aggagttcga ccagaccaag tgggggatcg gcatcaaccg
      661 aggcttcaac accttgctgc cctatcacgc gcaggccgcc aaggacggtc gggtctggta
      721 cgggctccag gacaacggct cgggctacat cgacggcacg acgcgggagc agttcatgac
      781 cttcggagcc gacggcggcg tcgtcgccgt cgatccggac aacagcgact acgcctggac
      841 ggagtctcag ttcgccggca tgcgcgtgac caccgacggc gggcggactt ggacctccgc
      901 accgccgggg atcacgaact atcagttcat caacccgttc gtgatggacc cgaccgatag
      961 caatcacgtc atgaccggcg ggcgccaggt cgtggagacc gtggacggcc ctcggacctg
     1021 cacggccgtg caagcggccg gagagtccga aaccatcgag tgcagctggg tgaaggtcta
     1081 cgacctggga acccacgcca agccgggaga cgcgtcggcg tcggcgaccg ggagcgatcc
     1141 ggcgaactcg acgacctcgg tcgcgcttca gggcgcgaac gcctacgtcg gtttctgcgg
     1201 accctgcgac atcatcaaca cgaccgttcc gtacaagcgg ggcctcgcga cgaacgtggg
     1261 cggtgacgcg ccggccgcga aggcatcgcc ggacggctgg cacatcgcct cggccaaggg
     1321 tctgccgaac cggacgatca cgagcatcgc gatcgacccg aagagcccga agacgatcta
     1381 cgtcaccctc ggcgggtact cgggccggac gtggcgcggt ccgggcacct tcgccgacaa
     1441 gaacaagaac cggggcacgg ggagcgtgtt cgtcagcacc gacgcgggcg agacgttcaa
     1501 gaacatctcg ggcaacctgc cgaacgcgcc cgcgaacgca atcgtgatcc gcggcagcca
     1561 gctgatcgtg ggaacggaca tcggcgtgtt catgtccaag gatctcagcg gcagcgcttg
     1621 ggcggcgctg gccaacgggc tgccggtcgt gccggtcgtg tcggtccagg tcatgccggg
     1681 cgagaccgac cggatcatga tcgcgacctt cggtcgcggc gtctacacct acaagattcc
     1741 ctccgcgctg tccccgcctc cgccgccgcc cgcgcaggtg aagggcgtca agaaggagct
     1801 cgcggcgacg ggtgtggacg actggttcgc agccggcgtg atcctggctg cgatggccgc
     1861 gttcctgacg acccgcctcc ggcggggccg tccccaggcc tagcaggccc ggcggaacta
     1921 cccggcgcgc ggccgtgcgc gccgggtaga ctctgccacc gttatggcga tctccgacga
     1981 gcaagtcctc cacgtcgcac gcctggcgcg cttgaccctg accgacgaag aggtcggcaa
     2041 gttcgcgcgc cagctctcct cgatcctcga gcatgccgag cgcgtcatgg cgctcgccgc
     2101 cgaggacgtg ccgcccacct cgaccgcgat cccgctggtg aacgtgctgc ggcccgacgt
     2161 ggcgggcgag tgcctgccgc aggacaaggc gctgtcgacc gcgcccgaat ccgagcaggg
     2221 ccgcttcaag gtcccgcgca tcatggagga gccgcaatga gcgatctgtg gcgcctggac
     2281 ggcgcgacgc tcgcggcgat gatcgccggc ggtgaggtga gcgcggccga ggtcgccgac
     2341 gcgcacctgg cgcgcatcga ggccaccgat ccggatgtgc acgcgatgct caccgtgacg
     2401 gccgagcacg cgcgcgcgca ggccgagcgc gtggacgacg cgcgcgcggc gggtgagccg
     2461 ctcggcccgc tcgcgggcgt gccgctcgcg ttgaaagacg tgctgtgcac cgacgggatc
     2521 cgcaccaccg ccgggtcgaa gatcctgggc gagtacgtgc cgccctacga cgccaccgtc
     2581 gtgcagcgca tgaacgacgc cggcatcgtc acggtcggca aggccaacat ggacgagttc
     2641 gcgatgggct catcgaccga gaacagcgca ttcggcccgt cgcgcaaccc ctgggatctc
     2701 gcgcgcgtgc cgggcggctc cagcggcggg agcgccgccg cggtcgccgc gggccaggcg
     2761 ccgcttggca tcggcagcga cacgggcgga tcgatccgcc agcccgccgc gctgtgcggc
     2821 atcgtcggcg tcaagcccac ctacgggctg gtctcgcgct acgggctgat cgcgttcgcg
     2881 tcctcgctgg atcaggtcgg tccgttcgcg cgctccgtgc gcgacgccgc gctgctactg
     2941 cagtcgatcg ccggccacga cccgcgtgac tcgaccagcg tgccgggcga cgcgcccgac
     3001 atggtctcgg ggctcgacga cggggtgaac gggctgcgca tcgggctatg gaccgagtcg
     3061 atgggtgagg gaacgcagca gggggtgcgc gaccgcgtgc gcgaggcggc cgaccggctc
     3121 gccgggctcg gcgcctcggt cgacgagatt tcgctgccgt cgttcgagta cgcgctgtcg
     3181 gcctactacc tgatcgcgcc cgccgagtgc tcctcgaacc tcgcgcgcta cgacggcgtt
     3241 cgatacggca tgcgcgccgg cggccgcgac gtggccgaga tgaacagccg cacgcgcgcc
     3301 gaaggcttcg gcgacgaggt caagcgacgc gtgatgctcg gcacctacgc actcagcgcc
     3361 ggctactacg acgcgtacta cggcaaggcg cagaaggtcc gcacgctcgt gatccgcgag
     3421 ttcgagaagg cctacgagcg cttcgacctg atcctgtcgc cgacctcgcc gacgaccgcg
     3481 ttcaagatcg gcgagcgggc cgacgacccg atctcgatgt accttgcgga cctgttcacc
     3541 atcccgtcca accttgccgg tgcctgcggg atcagcgtgc cgtgcggcct tgccgaggac
     3601 ggcctgcccg tcggcctgca gctgatgggc ccgccgatgg ccgagccgct gatgttccgg
     3661 gccgccaacg ccttcgaagc cgaccgcggc ccgctgcccg atccgccgct atgggcgtga
     3721 ccgccgtgga cgcgcagtcg aatggcgccc cgcagtggag cggccgggtg cccaagcccg
     3781 ccgcgatcct gatgctccaa atcgtcggtg gcttcttcct gatcgcgggc ggcctggcat
     3841 tctggctcgg atcggcgctg gactgggagg ggttggccaa ggcgtacgcg atcatcggcg
     3901 ggtacctggc cctgaccgga ccgttcgcgc tcgccgcggg ctggtcgcgc ccgcccgggc
     3961 tggtggccgg catggtggcc ctcgctttga tgctggcggc catcccgatc ggcccgatcc
     4021 tggtcgcggt gatcggcttc ctcggcatcc gcaaccgcga gcacgtccgc gactactacc
     4081 ggcaaaggat ccccaagcga tgagcgtgtc agattacgaa ccggtcatcg ggctcgaggt
     4141 gcactgcgag ctgagcacga actcgaagat gttctgctcg tgcccggcgc acttcggggc
     4201 cgagccgaac acgaatgtgt gtcccgtgtg cctcggcgaa cccggctcgc tgcccgtggt
     4261 caacgagcgg gcggtcgaag acgcgatcaa gatcggtctg gcgctgaact gcagcgtcgc
     4321 gccgcgcacg atcttccacc gcaagaacta cttctatccg gacatgccga agaactatca
     4381 gatctcgcag tacgacaacc cgatctgcac cggcggctat ctggacgtgg acggcggccg
     4441 ggtcggcatc acgcgcgtgc acatggagga ggacaccggg aagtcgctgc acgtggggtc
     4501 gagcggccgc atctcggagg cgacgcactc gctggtggac tacaaccgcg cgggcgtgcc
     4561 actgatggag atcgtctccg aacccgacgt gcgcagcgcc gagcaggcaa tgacctacct
     4621 gaccgagctt cgctcgatcc tggcgacgct gggcgtatcg gacgtgcgca tggaggaagg
     4681 ctccctgcgc tgcgacgcga acatctcgat ccacaagccg ggcacgcctt ggggcacgcg
     4741 cgtcgaggtc aagaacctga actcgattcg ctcgctcggc cgcgccgtcg ccttcgagat
     4801 cgagcgtcag gccaagttgc tggacgcggg tgagccgatc gtgcaggaga cccgccactt
     4861 cgacgagtcc accggcgtga ccaagacgct gcgctccaag gaagaggcgt tcgactaccg
     4921 ctacttcccc gagccggatc tggtgccgat cgagccggac gaggcatggg tcgccgggct
     4981 ccgcgccgcc ctgcccgagc ttcccgccgc gcgacgcgtg cggctcgaga ccgagcacgg
     5041 gctggccaca gagcacgccg cgttcgtcgg cgcgtcgccg gcaagcatga gctacttcga
     5101 agcggtcatc gccggaggca cacccgcgcg tgacgcggcc gtatggatgg cgggcgagct
     5161 ggcgcgctgg ctcggcgacc gcggtgccga catcgcggac agtcccgtct cgccgaaggc
     5221 gctcgccggg ctgatcaagc tggtcgggga gggcgcgatc aacctcaaca cgggcaagcg
     5281 cgtccttcag ctttcgttcg agtccggcaa ggatccggcg accatcgtgg ccgagcaagg
     5341 cttggagcag gtcagcgaca ccggcgcgat cgacgcgatc atcgccgagg tcctgaccgc
     5401 caacgcggcc gaggtcgagc ggttccgtgg cggcgaggag aaggtcttcg gattcctggt
     5461 cgggcagagc atgaaggcgc tcaaaggtca ggggaacccc aagctggtta acgaacgtct
     5521 tcgtgcggcc ctggagggat agtcgatggc ttcgaatgcc gagcagctgc tggcccaatc
     5581 ggttcgcaac cggccggaca ccgactaccg cttcgcatgg ggcacctaca tcggatggac
     5641 catcctcacg atgggcatct acagccacta cgcgacgtat cgcctggtgc agcgacgcgc
     5701 cgagcacgcc cggcgccggc tcgccttctc gtcgtacctg tggcaagttc ttgccgagcg
     5761 cgccgacgcg gccgacaagc gggatctggt gcagcccggg ctcgacaacc tgtcccgcgt
     5821 gcacgccgag atcgagtcct tcgagcgccg caacaagcgt gagccgggcc tctggaccat
     5881 cctgcgcgtg gtgttcacgg tggttggtgc ctacatcaac catttcctga acaaggactt
     5941 ccggttcctc gagacctggg aggtggcgct ggccgagaat gccgagtggg tcatgcagcg
     6001 cctcgagatc ggcggcccga tcgcgaagcc ggcgcatcgc acacccgacc gcagcacctg
     6061 gctctacgtg ctcctgacgg tcttgaccgc gggcctgttc tcgatctggt ggcgctccgt
     6121 gatcatgaag gacggcaacg agcacttcga cgccgaggag cgaagcgagg acgcgatcct
     6181 tgccgcgctc ggcatcgcgc cgcagacgat gacgccggcc cctccgcagc cgccgctcta
     6241 gctcatccga gcgcgatcag ggtcgcgatc gcggtccagg acgcgagccc gaaggccccg
     6301 agccagatcc cgatcgcagc gagcctgcgt ccgccccggt ccggtgaccg cgacagcgct
     6361 cgcttcgagc gcagccccag cacgatcgcc gcgatgctgc acacgaacgg gatcgcgagc
     6421 ccgagcactg caagtaccat cgcagcgggg gcgcggtcct cgaccgggac cggcatcggc
     6481 cgccgcgcct gcgcgatcgg ggcgcgaacg tagccgcagg ccgcgcaggc cgtggcatcc
     6541 ggatcggccg gcgcgccgca tcgtggacag gctgctcgca tcggattgcc gagcctgccc
     6601 gacccgcctg cggcggtcaa cttcgccggt gcggcagaat ggacccgcat gtccatgagc
     6661 cccgagcagt tcttgctggc cgagatcggc cgccgcgacg cgaccgacta ccgattccgc
     6721 tggggaacct acttcggctg ggcggtgctg accggcggtc tgtactcgat ctacggcacg
     6781 ctcaagctgg tcgagcggcg cgtcgatcac gttcgccggc ggctcgcggt ctcgacgtcg
     6841 ctgtggcacg cgctggcggc gaaggcggat tcgctgcgcg tgcgaggcga tgtccaggag
     6901 ggcctcgaca acctgtcgcg catccatgcc cagatcgaga cctacgagcg caagaaccgc
     6961 cgcaacgccc agaccaccgc cgcggtccgc gcggtcctgc tgttcgccac cttcgccgga
     7021 atcgcgatgt tctccgcgct ctacgatccg gatctcggcg tggccggtca gcccgatctg
     7081 gtggcggtcg gctcgctgat cttcgacgcg ggtgtgatcg ggctgctcgt gcacggagcg
     7141 atcacgaacg cggcgctgca tcgcgacttc aacttcctcg acacctggga gacctcgtgg
     7201 gccgagaacg tcgagtgggt catgtaccgg ctcggcatgg cgccgtcgtt tccggcccgg
     7261 ccgccccagc ctacgcgatc cacggcgacc tacgtgctgc tgacggtgtt ttcgctcggc
     7321 gtcttctcga tcttgtggcg tgcctcgttg atgggcgacg gcaaccgcca cttcgaccac
     7381 gacgacgagg tcgaggacgc gatcctggcg gcgatggatc tgcgcggatc cccggcgccc
     7441 cagaccggcc tgccggtgct gccgccgtcg taggcggtcc gggccgctgg cgggcctgcg
     7501 atatcctgct cgccatgagc gatccccagt tcgatccgcg gcaccgcagc cgcgacgtca
     7561 ccgaagggcc gcagcgcgcg cccgcccgcg ccatgctgcg cgccgtcggc ttcaccgaag
     7621 aggatttcgg caagccccag gtcggcgtcg cttcctcgtg gaacgaggtc acgccgtgca
     7681 acgtgcacct gaacacgctc gccggctacg cgaaagaggg ggtgcgcgcc gcaggcggcg
     7741 tcccggtcga gtacacgacc atcgccgtgt cggacggaat cgcgatgggt cacgaaggca
     7801 tgcgcgcttc gctgatcagc cgcgaggtca tcgcggactc ggtcgagctg gtcgcgcacg
     7861 cggagcgctt cgacggtctg gtcacgatcg cgggctgcga caaatcgctg cccggcatgg
     7921 tcatgggtgc ggcccgcttg gacatcccga gcgtgttcct ctacggcgga tcgatcatgc
     7981 ccggccacta cgccggccgc gacgtcacga tccaggacgt gttcgaggcc gtgggcaccc
     8041 actcggccgg aaacatgacc gacgacgagt tgctcgccct cgagcgggcc gcctgtccgg
     8101 gtgcgggctc gtgcggcggc atgttcaccg ccaacacgat ggcggcggcg atcgaggcga
     8161 tgggcctgtc gctgcccggc agcgcgtccc cgcccgcgct ggacgcccgg cgcgacgaat
     8221 tcgcacgcgc cagcggcgag gcgctgctgc ccatgctcga gcagggactg acgccgcggc
     8281 gcatcatcac ccgcgcctca ctcgagaacg ccatcacggt ggtcgtcgcg tgcggcggct
     8341 cgaccaacgc ggtgctgcat ctgctcgcca tcgcgcgcga agccgagacc gagctcgagc
     8401 tcgacgattt cgatcggatc gcccggcgaa ccccgatcat cgtggacgcc aagccgtggg
     8461 gtcgcttcgt catgctggac atcgacaagg cgggcggcat ccccgccgtg atgcgcgagc
     8521 tgctcgacgc ggatctgctc aacggcgacg caatcaccgt cacgggcaag agcatcgagg
     8581 agaacctgcg ggacacggcg ctgtcgagcg accgcagcgt ggtctacccg gtggcgcagc
     8641 cgatctcgcc gtacggcggc ttcgcgatcc tgcgcggcga tctggctccg gacggctgcg
     8701 tggccaaggt cgcgggcggt cacggctcct tctcggggcc cgcccgtgtg ttcgaccgcg
     8761 aggaggatgc gatggctgcg gtcatccagg gacggatcaa cgcgggcgac gtgatcgtga
     8821 tccgctacga ggggccgcgc ggcgggccgg gcatgcgcga gatgcttgcg gtcacggcgg
     8881 ccgtgaacgg ggcgggcctg ggcgagcacg tgtgcctgat caccgatggc cgcttcagcg
     8941 gcgccacgca tgggaacatg atcgcccacg tctcgcccga ggcagccgtc ggcggaccga
     9001 tcgcgctcgt cgccgaaggt gacgcgatca ccgtggacgt accgaaccgg cgcatcgacg
     9061 tagcggcgga cctcgaagca cgccgcggcg cctggcggcc gcccgccccg aggtacacaa
     9121 aaggggtttt cgggaagtat gctacccgcg tgagctcggc cgccgaaggg gctgtctggc
     9181 ggtaggcgga ccctcctttc cgcggccggg cggagccgcc gaccagggga gagctgccgt
     9241 gaaagcacgc gccatcgccg ccgcgatcgt gaccgcctcg ctcgttgctc ccgcggcggc
     9301 gaccgatcac ctgccgtatc ccgcgtactc gtcgtgcacg gagcgtttca tcgaaggacc
     9361 gatctcgtcc tcgtgcccgt tcgagtacgg cggccgccgg gtcacggtct tcggcgccgg
     9421 gaccgcctgg ggcgagccga tgtcgatcac gctcgagatc gtccttcgtg aagggcccga
     9481 cgagcaggtc ctgttcgtgt gcagcgcccc cgccaccggg gcgggtccgc tcagcgcgcc
     9541 ggccgcacaa tgcgtgcggc gctctcccgt gctgcaggtg gctccgggta cgatgctgcg
     9601 ctgccggcac cgcggccccg gaaccggcgt gttcggctgc tcctcgccgt gacgcttacg
     9661 agcgccgcag cgaccgaacc gcgtcggtga gcgagaaccg cgcgccgtcg gcataacgcc
     9721 gcgcgccgct ttcgctccac acgaccacgc cgccgcgtcc gcgcaccagc gcctcggtct
     9781 cgtccagcag cagcgccgag tcctcgtcca cgccgaagac cgtgcattgc ggcgagatct
     9841 cggcggcgag ctgcgggcct tcgagccaaa tgtcgtagtg gggcacggtg gcgatgccct
     9901 gcagcatgcc caggccgatc gtccagatcg tcggcgtgcc gtgcggatcc gcctcggtgt
     9961 ggacgagcgt ccagtcgccg agcaccatcg cgcccgccga cgcgcccacg atccacgcgc
    10021 cgttcgcccg cgcccgcagc atgccgtcga ataggggaga ggcttcgatc gcggccatga
    10081 gccgcgccgg cttgccgccc gagaagtaca cgagatcggc gcgttcgatc tcggcgacgt
    10141 gcgcgggatc ggaggcgtgg atgcggtcca gcaccatcag cgcgacgctt tgtgcgccga
    10201 gccgggtgaa atgctcgacg ccccgatagg cccagtcccc gggatcctcg ccgcccgcgg
    10261 ccgtcggcag gacgacgaca cgcggcgagg gcccgatcga ccgcagcagc tcgcgatcga
    10321 tcccggtcat cgccggtgtg aactcgcccg atccgaccaa tccgatgcgg gcgtgcgaca
    10381 gcggattttc gatggcctcc tcctcgcgcg ccgccgcggc aggcggcgcc ggcgcggaag
    10441 attactcggt ctcgggggcc ggcttctcgg tcaggacgat cacgcccgcg ccgtggcatt
    10501 cgggcacgcc gtctgccggc gggcacaggg ggtcgctcgt gacgatcgcg gcttcgacca
    10561 gatccccggg ttgggacggg atcgtccaag tgctcggcgc gctgccgccg taggaccaga
    10621 cgagctcgga atcccgatag atgcgcacga cccacggccc gacgcgggcg gagtcgaagg
    10681 cggccggtcc cgcggcaaag aatccgcagg cagggttggc ctcggagacg atgcatcgga
    10741 tttcgccccg tggcatctct tgggcgatgg ctccgccggt aagcgcagcc gcgaccagcg
    10801 cggcggccat aatggctcgt gcgatcatga tttctccccc tcgcaggtgg cccttgcaat
    10861 ggcttcgccg tccctttcgg cctccgcgcg gccgcttccc ccgtcgggac gcacccacaa
    10921 atcggtctat ttccgtccga agcgatgctg cttgacactc cggcagggcg gcggacataa
    10981 tccggcaacc atgaagatca ccggaaaggg ccgcgagacg acgttcgtcg tcgtagttct
    11041 ttcctgagcg cgttgcgcct gcgggcgcgc gcgccgacct ctcgaactcg agaggttttt
    11101 tgtttccggg gaaaatgcag ttcagagaac cgagcagcgg gccgaaaacg ggatgaaagc
    11161 ggaaggtgac acggaatgaa ccgcacggcg ttgatcatca tcgacatgca gaacgacttc
    11221 gctcatccgg acgggcgggc cttcttgccc gacgccgccc acaaggtccc ggtcatggcg
    11281 tcggtgctcg acgcgttccg ccaggccaag gccccggtca tccacgtcgt gcgcacctac
    11341 cgccgagaca cgtgggacgt ggagcgattc cgggtgccgt atttccagaa ccagccgggc
    11401 ttcctcgtcg agggaacctg gggcgcgcag atcgtggacc ggctgacccc ggagcccggc
    11461 gagccggtgg tgatgaagtc gcgcttcagt gcgttcgcct tcaccaacct ggatctgctg
    11521 ctgcgccgcg ccggcgtcaa caagatcgtc gtcgtcggcg agaacctgcc caactgcccg
    11581 cgcacgacca tgttcgacgc gatcgggctc gactacgacg tggtcgcggt tccggacgcg
    11641 ctcgccacca ccggtgacga gacccaccac tacaacttgc aggacctcgc agcggtcggg
    11701 gtccgggtcg cgccctcgca ggacgtgatc gccgagattc tccgcgaagg agaccgctac
    11761 gcggatgcca cgcacggcgc aatggctgcc cgccgcgacg aagaacggag tgaaggccga
    11821 tgaagctgac cggagcgcaa gcagtcatca agtcgctcga gcaagagggc ggcgaggtga
    11881 tcttcgggat tcccggcggc gccatcctgc ctgcctacga ccccctgctc gtgacggcac
    11941 aggat
//
LOCUS       DATLFO010000260        11745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88230, whole genome shotgun sequence.
ACCESSION   DATLFO010000260 DATLFO010000000
VERSION     DATLFO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88230"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VM841_10650"
     CDS             complement(<1..721)
                     /locus_tag="VM841_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90679.1"
                     /translation="MTLYDRIGRSYARHRAAEPAIEERIKAAFGDAVNVVNVGAGTGS
                     YEPRDRICVAVEPSPVMIEQRPPNAAPVVAAFAEALPFADDTFDVAMAVLTVHHWNDP
                     EAGLREMRRVASRVVIFGFDLVALHERFWLIRDYLPELLTLESGSPTVERAAEITGAA
                     SIESVPLPATCRDGFMPAYWARPEAYLDPSVRSAISVFHRLDPAIVRRMVMKLSEDLG
                     SGRWDKRYGSLRDTKELELGFR"
     gene            complement(741..1811)
                     /locus_tag="VM841_10655"
     CDS             complement(741..1811)
                     /locus_tag="VM841_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVL90680.1"
                     /translation="MNIEELLRTAVERRASDLHLRAGGPPYMRVDGDLFPVDAPALPA
                     TETERLAFDLMSDKQARQFQSTNECDFAFTVPGLSRFRVNVFRQRGSVGLAIRRVLPG
                     GSSFEALNLPPAVRRLAAELRGLVLVTGATGSGKTTTTAAMLHHINMSRRCHVVTIED
                     PIEILHSDRMAIIDQREVGIDTEDFHSALKYVMRQDPDVIFIGEMRDVETVRAALAAA
                     ETGHLVISTLHTLDATETVNRIIDFFPPHQQQQGRLALAGTLKGIVSQRLLPRADGKG
                     RVPAVEVLVANGRIFEMITNSEHTHLIHDVIAEGDFYGMQTFDQNLMTLYQEGVVTLQ
                     DAMAAATNAHDFTVALRKVGLA"
     gene            complement(1974..2690)
                     /locus_tag="VM841_10660"
     CDS             complement(1974..2690)
                     /locus_tag="VM841_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVL90681.1"
                     /translation="MIERIVEWVTPVFAEWGLLLVFLATFLETSAFLGLIVPGETVVI
                     LGGILANPTFRGEFTEAGSLEITIQQVIVVAIVGVIAGDQAGYMIGRVGGRKLAHRWK
                     RLLRPERLAAAEAYYRQHGGKTVFLGRFTPFLRSLGAMVAGMSRMRYWRFTLWDLAGA
                     ILWGIGIPMVGYIFGRYGTASIKAIDRFLKAPGFALLALLTIGMFIYWRRRKKERELA
                     GDSWRREEAAAGKSRKPNVG"
     gene            2850..3212
                     /locus_tag="VM841_10665"
     CDS             2850..3212
                     /locus_tag="VM841_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90682.1"
                     /translation="MNEERNVMEDLRSRFDQRTEPEAGAETAPEAARGAAMEYPRSEA
                     ASRSDERDHRIEGDAPRRKMTRAEAGRKGGLRTKERHGQDYFGRIGRMGGKKGGEATK
                     QRYGVEFYRSIGRKGGSR"
     gene            3298..3723
                     /locus_tag="VM841_10670"
     CDS             3298..3723
                     /locus_tag="VM841_10670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL90683.1"
                     /translation="MKTSTATSAGGVVFDGDERVVLLGHTTPNGKQRWSLPKGAVEAG
                     ETPEQAAVREVREETGLDAEIVAKAQTIDYWFVWAPDETRYHKYVHYFAMRMTGGDFS
                     RRDDEAERVEWFSVEDALKACSYPNERATIRKALASIRA"
     gene            3716..5809
                     /locus_tag="VM841_10675"
     CDS             3716..5809
                     /locus_tag="VM841_10675"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HVL90684.1"
                     /translation="MRRLRAAALLAALCAIAVAGPAAGQSASPEPAASVRITSFTPYN
                     TLETPFRLSLRLRNTGTVPIEDARIRLTIYQRVLSRSALNASLQGRPQTVVAGETTEW
                     LSETVTPDTTVVLPLERDLARVSSVFAARRSSRPGVYPVRIRAEGSDRELWTGYSAIV
                     FLPTPPEERLNVTWVLPVHHKALFDGDGTYPGGTVDEALGPEGVLSDRLAAAADASSL
                     RVTYAPSGLLADQLADLTDGFARRNGRQILQSVAGDAIPRAAGAAVGRIKAAMAKSSV
                     EIAATPYSRADLVSLIHAEMADDVGKQVSMGLQTVSEVFGRTPNVSVFVPAGLRIDSR
                     AAAAVAGYGVRTVVMDPSILPPHPGPFGHDRPVRVSGRGGVRLNVLPADEAIRAHLEG
                     ASAPNGGDPVLLAQSALAETASAWLERPTLADGRAIVIATATTPPAASIRALSDALSA
                     APWVSMRSASELVEVVPPEEDPEPLPLGNIGPRAEMQTLRTARRAIDILSRVVVQPPD
                     PAADLDRLVLAAASADWAGTGAGTDMARSAKQTADRIVSQIGAAARRVTLTSRTGQLP
                     VTILNETGYAVAVQVRLLSSKVDFPDGERLTVQVPGRSETVAVPAKALAAGTFGVKIE
                     LRTPDGTYPIGDDELVVRSTAVSAVALIAVGGGAAVLIAAWFRRGGKRRRGTPAAEKA
                     PRKTNPATGERPQPA"
     gene            5824..7521
                     /gene="murJ"
                     /locus_tag="VM841_10680"
     CDS             5824..7521
                     /gene="murJ"
                     /locus_tag="VM841_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HVL90685.1"
                     /translation="MATDGPAEDAPAPASSAPSLARSTAVMSVGTAASRITGFVRLSV
                     MAWAIGGAESKLPDTYNLANSLPNIVYQLVLGEILATVFVPVFVEYIKTRARDEAWKL
                     ASTILNAAFLIAGLFAIVTVIAAPWIIKIYTFRVPDPVVRAEMEATGAFFLRIFMPQM
                     IFYAMGAVLTGLLNAHRKFAAPMFAPVLNNLIVIATFIVFRSMHGGGVPNLATLTGAE
                     KWVLAGGTTLGVIVFTMVLWPFVRKLPGGYQPRAWEWRHPAVRHVGTLAKYSFGYVII
                     NQLGLWVVYMLANRTEGGISAFQAAFILYQLPYGIFAVSVMTALVPSLAEHHVNGDLR
                     RFREDLSLGLRTTAFVVLPAAAGFVALGAPIIRLLLEHGVFSAESTKLYADTFVLMAA
                     GLYAYAAFQQIMRAFYARQDTKTPWIVNGVAVLINIAVDVPLYRSMGVPGLALGHTIS
                     YVFALAYGGYLLRRELKGIDGRRLLSSHLRIGLASVVTAAASWAVARLVERTVGVETL
                     GGQLLQVGGAVTAGLILYASLSIALRLDEFRPLVRMVTGRFGRRASTRNGSQEGSGS"
     gene            7518..7913
                     /locus_tag="VM841_10685"
     CDS             7518..7913
                     /locus_tag="VM841_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90686.1"
                     /translation="MSTAAIEPRQRGGIITGWLVRLAVSLLLLGLAFFEAGAVIVARV
                     GVDGTAQTAAREAALVYGNSRDPQAAEAEAAKAARQGGARLVEFTISTEGKQVTVTLE
                     RTAKTFVIHKIGPLEKFATTRASDTADVR"
     gene            7917..8987
                     /locus_tag="VM841_10690"
     CDS             7917..8987
                     /locus_tag="VM841_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HVL90687.1"
                     /translation="MDVRKAGPSERDAFNGFVSAMPSGDLLQSWEWGEIKRASGWAPA
                     RYLVMDGGRIVAAASVLRTRPLKGAPPLLYAPRGPVFRPEAAGAVRALAEQIRRDAGS
                     AFVLKCDPAIEAGSAEAGALREAGFRPAGGAGFGGVQPKAVMVLDIGRDEDAILEGFK
                     SKWRYNIRLAERKGVTVEQAGREDLPAFYDLLLITAKRDGFFVRGRAYFETLFDTLEP
                     RDMLSLWLARYEAAPVAGAICFAFGNRVTYVYGASSNEHRKVMPNHLMQWTMIQWARK
                     RGATLYDFRGVSPVRDGKPVEEHIAGLNRFKEGFGARYVEYTGDLDLPLRPLVWRAWV
                     AGAPAAMKLYKKIKGGAGAAAD"
     gene            8987..10105
                     /gene="alr"
                     /locus_tag="VM841_10695"
     CDS             8987..10105
                     /gene="alr"
                     /locus_tag="VM841_10695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVL90688.1"
                     /translation="MRPEADATTWVAVSAGALERNYRAVANYAGTTVCAVVKANAYGH
                     GLAEAARTFESAGARWLAVTRVEEARTLRGAGIESHILILTPPPTAGLAAEAVSLDAA
                     LTASDAADVDRYAAAARDAGRPARVHLKIDTGMGRLGVPVSEAAAVAERIAAEEQVSL
                     DGVFTHFAGAAGPSGAAQLSRFRAVREALGKHAARAIVHASNSAALLKLPGARFDMVR
                     IGTLLYGQNPPGATAPFSLEETFQWYARVVAVRTVPAGTKVGYGGEWTAKKPVRLATI
                     PIGWGDGFTAEPHARSETLAAVATGAAKAIAVAAGARPTPRVVLFGSQKAKVVGRAGM
                     QAITVSLEGLPGVAVGDVARIPARRLLVAGHIDRIYGA"
     gene            10116..10718
                     /locus_tag="VM841_10700"
     CDS             10116..10718
                     /locus_tag="VM841_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL90689.1"
                     /translation="MIEERTDHELIAAHLGGDRHAFDRIVERYEKRVWAVCLRMCGDP
                     DDARDAAQDTFVTAFRSIGSFRGDAQLSTWLHRIAVNASLDVLRKRGRNKSQPVEDVP
                     EIASDEAGPEEQAVRASRAAAVHAALTRLSEEHRTVIVLHDLQGLQYPEVAEALDVPV
                     GTVKSRLHRARTEMARLLGHLKDDGTEGPASPSNVSRNRR"
     gene            10715..11479
                     /locus_tag="VM841_10705"
     CDS             10715..11479
                     /locus_tag="VM841_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVL90690.1"
                     /translation="MTTHPEHEQLSAYADGELGATERVAVEAHLAACGKAREMLAAIR
                     ATLADFAALDEVEMDPQVGFAIRSALARERKLSSRTRRYAWAGGAAAAALVGILSFAI
                     LGGGPNPARDASGAGLGGGPQLMAMEDLDEDSALRVLETYVQENYAFPGARGDMGGDD
                     AAAETAVLTHAPPAPEIQAYSAADATTFADCIRTIERESDSARLVRSIAATYKGEPVY
                     VLIFEIPAERPDRVEMWITARDSCETRYFSQANVNR"
     gene            11578..>11745
                     /locus_tag="VM841_10710"
     CDS             11578..>11745
                     /locus_tag="VM841_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL90691.1"
                     /translation="MTTPENVIIIGSGPAGLTAALYAARAQLDPLVIEGAAPLGQLML
                     TTEVENYPGFPE"
BASE COUNT         1844 a         4128 c         4050 g         1723 t
ORIGIN      
        1 ggcgaaagcc cagctccagc tctttggtat cgcgcagcga gccgtagcgc ttgtcccatc
       61 ggcccgagcc gagatcctcc gacagcttca tcaccatgcg gcgcacgatc gccggatcca
      121 ggcggtgaaa caccgagatc gccgaccgca ccgacggatc caaatacgcc tcgggacgcg
      181 cccaatacgc gggcatgaac ccgtcgcggc acgtagccgg caacggaacc gattcgatcg
      241 aggcggcgcc ggtgatctcg gcggcgcgct cgacggtggg cgacccggac tccagcgtga
      301 gcagctcggg cagatagtcg cggatcagcc agaagcgctc gtgcagcgcg accagatcga
      361 atccgaagat caccacccgc gacgcgacgc ggcgcatctc gcgcaggccc gcctcgggat
      421 cgttccagtg atgcacggtc agcaccgcca tcgcgacatc gaaggtgtcg tcggcgaacg
      481 gcaacgcttc ggcgaacgcg gccacgaccg gcgcggcatt cggcggacgc tgctcgatca
      541 tgaccggaga cggctcgacc gccacgcaga tgcgatcccg cggttcgtag gagccggtcc
      601 cggctcctac gttgaccacg ttcaccgcat ctccgaacgc cgctttgatg cgctcctcga
      661 tggccggctc ggccgcgcga tggcgcgcat aggaacgccc gatgcgatcg tagagagtca
      721 cgccgctagg tctatcagac ctaggccagg ccgaccttgc gcagcgccac cgtaaagtcg
      781 tgcgcgttgg tcgccgcggc catcgcatcc tgcagcgtga ccacgccctc ctggtagagc
      841 gtcatcaggt tctggtcgaa ggtctgcatg ccgtagaagt cgccttccgc gatcacgtcg
      901 tggatcaggt gcgtgtgctc ggagttggtg atcatctcga agatgcgccc gttggcgacg
      961 agaacctcga cggcgggcac gcggcctttg ccgtcggcgc gcggcagaag ccgctgcgac
     1021 acgatgccct tcaaggtgcc ggccagcgcc agacgcccct gctgctgctg atgcggcggg
     1081 aagaagtcga tgatgcggtt gacggtctcg gtcgcatcca gcgtgtgcag cgtcgagatc
     1141 accaagtggc cggtctcggc cgcggccagc gcggcacgga ccgtctcgac gtcgcgcatc
     1201 tcgccgatga agatcacgtc ggggtcttgg cgcatgacgt atttcaaggc ggagtgaaag
     1261 tcttcggtgt cgatgccgac ctcacgttga tcgatgatcg ccatccggtc gctatgcagg
     1321 atctcgatcg gatcttcgat cgtgacgaca tggcatcggc gcgacatgtt gatgtgatgc
     1381 agcatcgccg cggtcgtggt ggtcttgccc gagccggtcg cgccggtcac cagaaccagg
     1441 ccgcgcagct cggccgccag ccgccgcacc gccggcggaa ggttcaacgc ctcgaacgag
     1501 gagccgcccg gcagcacgcg ccggatcgcc agacccaccg agcctcgctg acggaagacg
     1561 ttgacgcgga atcgcgacag gcccggcacc gtgaaggcga agtcgcattc gttggtggac
     1621 tgaaactggc gagcttgctt gtcgctcatc agatcgaacg caagccgttc ggtttcggtg
     1681 gccggcaggg ccggcgcgtc cacggggaac aggtctccgt ccacgcgcat gtacggaggg
     1741 cctccggccc gaaggtgcag gtccgatgcg cgacgttcga ccgcggttct gagaagctct
     1801 tcgatattca cggggcctcc gggcaggcgt cctgacggcc tccggtgcgg gccgacgcta
     1861 ttggaatcgg cgcagccggc ggcgcccttg acctgcgaag cgagcggcgg agccggatcc
     1921 ttcactttcg ggggtccaga cggcgtggat gtgacgggta cgggcggacc gccctatccg
     1981 acattgggct tgcgcgactt gcccgcggcg gcctcttctc gccgccagga gtcccccgcg
     2041 agctcgcgct ccttcttgcg gcgccgccag tagatgaaca tgccgatcgt caggagagca
     2101 agcaaggcga acccgggagc cttcaggaag cggtcgatcg ccttgatgct ggccgtgccg
     2161 tagcggccga agatgtagcc gaccatcggg atgccgatcc cccacaggat cgcgcccgcc
     2221 agatcccaca gcgtgaagcg ccagtagcgc atgcgggaca tgcccgcgac catcgcgccc
     2281 agcgagcgca ggaacggcgt gaagcggccg aggaacaccg tttttccgcc gtgctggcga
     2341 tagtaggctt ccgcggccgc aagccgctcg ggccgcagca gccgcttcca tcgatgggcg
     2401 agctttctgc cgccgacgcg gccgatcatg tatccggctt ggtcgcccgc gatcacccct
     2461 acgatcgcca cgacgatcac ctgctggatg gtgatctcga gcgagccggc ctcggtgaac
     2521 tcgccgcgga aggtggggtt ggcaaggatc ccgcccagga tgacgacggt ctcccccggg
     2581 acgatcaagc ccagaaaggc ggacgtttcg agaaaagtcg caagaaatac gagcaaaagg
     2641 ccccactcgg caaagaccgg ggtcacccac tcgacgatgc gctcgatcac cgccgcaatc
     2701 atacgggccg cttgcgaccg gaccgttcct tgatccgccc taccccctct gacctgggca
     2761 cgtgcaaggc aggcgaaaag cgggttaacc tgtggtcgaa ccttaaaaaa ctcctatgtt
     2821 ggagtgcccg aagctggagg tggatatcgg tgaatgagga gcgcaacgtc atggaagatc
     2881 tgcggagccg cttcgatcag cggaccgagc cggaagccgg ggccgaaacc gcgccggaag
     2941 ccgcccgggg ggccgcgatg gagtaccccc gcagcgaggc cgcgagccgc agcgacgagc
     3001 gggaccatcg catcgaggga gacgcgcccc gccgcaagat gaccagggcc gaggccggcc
     3061 gcaagggcgg gcttcgcacc aaggagcgcc acggccagga ttacttcggc cgcatcggcc
     3121 gcatgggtgg caagaagggc ggcgaggcca ccaagcaacg ctacggcgtc gagttctatc
     3181 gcagcatcgg ccgcaagggc ggctctcgat agccgagcgc tgcctctgag agacgcaggc
     3241 acgacgaacc gaacgacgca cccgcgccgc cgcgctgcgc gccgtatcct cgttggcgtg
     3301 aaaacgtcca cggcgaccag cgcgggcggc gtcgtcttcg acggcgacga acgcgtcgtc
     3361 ctgctcggcc acaccactcc caacggcaag cagcgctgga gcctgcccaa gggggcggtc
     3421 gaggccggcg agactcccga gcaggccgcc gtgcgcgagg tgcgcgagga aaccggtctc
     3481 gacgccgaga tcgtcgccaa ggcccagacg atcgactact ggttcgtgtg ggcgccggac
     3541 gagacgcgtt accacaagta cgtgcactac ttcgccatgc gcatgaccgg cggcgacttc
     3601 tctcgccgcg acgacgaagc ggagcgagtc gaatggttca gcgtggagga tgcgctgaag
     3661 gcatgctcgt atccgaacga gcgcgccacc atccgcaagg cgctcgcatc gatccgtgcg
     3721 taggctccgc gccgcggcgc tgctcgccgc cctctgcgcg atcgcggtcg cgggaccggc
     3781 ggccggccaa tcggcgtccc ccgagccggc cgcgagcgtg cggatcacct cgttcacgcc
     3841 ctacaacacg ctcgaaacgc ccttccggct ttcgctgcgt ctgcgcaaca ccgggaccgt
     3901 cccgatcgaa gacgcccgca tccgtctgac catctaccag cgggtcctgt cccgctccgc
     3961 gctcaacgca tcgttgcaag ggcggccaca aaccgtggtc gccggcgaga ccaccgagtg
     4021 gctatcggag accgtgacgc cggacaccac cgtcgtcctt ccgctcgagc gcgacctggc
     4081 ccgggtcagc agcgtcttcg ccgcccgccg gtcgtcgcgg cccggcgtct accccgtgcg
     4141 aatccgcgcc gaaggctccg accgcgagct ttggaccggc tacagcgcga tcgtgttcct
     4201 tcccacgcca ccggaagagc gcctcaacgt cacctgggtt ttgcccgtcc accacaaggc
     4261 gctcttcgac ggcgacggaa cctaccccgg cgggaccgtc gacgaggcgc tcggacccga
     4321 aggcgtgctg tcggaccgcc ttgccgcggc ggccgacgcg tcgtcgctgc gcgtgaccta
     4381 cgcgcccagc ggcctgctgg ccgaccagct cgccgacctg accgacggct tcgcgcgccg
     4441 caacggccgg cagatcctgc agtcggtcgc cggcgacgcg atcccgcggg cggccggcgc
     4501 ggcggtcggc cggatcaagg cggcgatggc gaagtcctcg gtggagatcg ccgcgacgcc
     4561 ctactcgcgg gccgatctgg tctcgctcat ccacgccgag atggccgacg acgtcggaaa
     4621 gcaggtctcg atggggctgc agaccgtgtc ggaggtgttc ggccgcactc cgaacgtgtc
     4681 ggtgttcgtg ccggccgggc ttcggatcga ctcgcgcgcc gccgcggcgg tcgcgggcta
     4741 cggcgtgcgc acggtcgtga tggatccgag catcctgccg cctcacccgg gaccgttcgg
     4801 ccacgaccgg cccgtgcgcg tctcgggccg cggcggcgtc cggctcaacg tgcttcccgc
     4861 cgacgaggcg atccgcgcgc atctggaagg agcctccgcc cccaacggcg gcgacccggt
     4921 gctgctcgcg cagagcgcgc tggccgagac ggcttcggcc tggctcgagc gccccaccct
     4981 ggccgacggg cgcgcgatcg tgatcgcgac cgcgacgacg ccgccggccg cgtcgatccg
     5041 ggcgctctcc gacgcgctgt cggccgctcc ctgggtctcg atgcgaagcg ccagcgaact
     5101 ggtcgaggtc gtgccgcccg aggaggaccc ggagcccctg ccgctgggca acatcgggcc
     5161 gcgcgccgag atgcagacgc ttcgcaccgc ccggcgcgcg atcgacatcc tgtcccgcgt
     5221 cgtggtgcag cccccggatc cggccgccga cctcgaccgg ctcgtgctcg ccgcggcgag
     5281 cgccgactgg gccggcaccg gcgcgggaac cgacatggcg cgcagcgcca agcagaccgc
     5341 cgaccggatc gtgagccaga tcggggccgc ggcacggcgg gtcacgctga cctcccgaac
     5401 ggggcagctt ccggtgacga tcctcaacga gaccggctac gccgtggccg tccaggtgcg
     5461 gctgctgtcc tcgaaggtgg actttcccga cggcgagcgg ctcaccgtcc aagtgccggg
     5521 ccgatccgaa acggtcgcgg tgccggccaa agcgctcgct gcgggcacct tcggcgtcaa
     5581 aatcgagctg cgcacacccg acggcaccta tccgatcggc gacgacgagc tcgtggtgcg
     5641 ttccacggcc gtgagcgcgg tcgcgctcat cgcggtcggg ggcggcgcgg cggtgctgat
     5701 cgcggcctgg ttccgccggg gcggcaagcg gcggcgcggc acgcccgccg ccgaaaaagc
     5761 tccacgaaaa acgaaccccg ccacgggcga gcggccgcaa cctgcctgat acgatcgccc
     5821 gcgatggcga ccgacggtcc cgcggaagac gccccggccc ccgcatcctc cgccccttcg
     5881 ctggcgcgct cgaccgccgt gatgagcgtc gggacggccg cctcgcgcat caccgggttc
     5941 gtgcgcctgt cggtgatggc ctgggccatc ggaggcgcgg agtcgaagct tcccgacacc
     6001 tacaacctcg cgaactcgct gccgaacatc gtctaccagc tcgtgctcgg cgagattctc
     6061 gccaccgtct tcgtacccgt cttcgtcgag tacatcaaga cgcgcgcccg cgacgaggcc
     6121 tggaagctcg cctcgacgat cctgaatgca gcgtttttga tcgcgggcct gttcgcgatc
     6181 gtcaccgtga tcgccgcgcc gtggatcatc aagatctaca ccttccgcgt gcccgacccg
     6241 gtcgtgcggg ccgagatgga ggcgaccggc gcgttcttcc ttcgcatctt catgccccag
     6301 atgatcttct acgcgatggg agcagtcctg accgggctgc tcaacgcgca ccgcaagttc
     6361 gccgcgccca tgttcgcgcc ggtgctcaac aacctcatcg tcatcgccac gttcatcgtg
     6421 ttccgctcga tgcacggcgg gggcgtgccg aacctggcga cgctgaccgg cgccgagaag
     6481 tgggtgcttg cgggcgggac gacgctgggc gtgatcgtgt tcacgatggt gctgtggccg
     6541 ttcgtgcgca agcttcccgg cggctaccag ccgcgcgcgt gggagtggcg gcacccggcc
     6601 gtgcgccacg tcggcacgct cgccaaatac tcgttcggct acgtgatcat caaccagctc
     6661 ggactctggg tcgtctacat gctcgccaac cgcaccgagg gcggcatcag cgccttccag
     6721 gccgccttca tcctgtatca gcttccctac gggatcttcg cggtatcggt gatgacggct
     6781 ctggttccct cgctcgcgga gcatcacgtc aacggcgatc tgagacggtt tcgggaagac
     6841 ctgtcgctcg ggctgcgcac gaccgccttc gtggtgctgc ccgcggccgc gggattcgtc
     6901 gcgctcggcg ctccgatcat ccggcttctg ctcgagcacg gcgtcttctc ggccgagtcc
     6961 accaagctct atgccgacac gttcgtactg atggccgccg gcctgtacgc ctatgccgcg
     7021 ttccagcaga tcatgcgcgc gttctacgcc cggcaggaca ccaagacacc ctggatcgtc
     7081 aacggcgtgg ccgtgctgat caacatcgcg gtcgacgtgc cgctgtaccg gtcgatgggc
     7141 gtgcccggcc ttgccctcgg ccacacgatc tcctacgtct tcgcgctcgc ctacggcggc
     7201 tatctattgc gaagggagct gaagggcatc gacggacggc ggctgctgtc gagccacctg
     7261 cgcatcgggc tggcctcggt cgtgacggcg gccgcgtcgt gggcggtggc gcggctggta
     7321 gagcgcaccg taggcgtcga gacgctgggt ggccagcttc tgcaggtcgg gggggccgtg
     7381 accgcgggac ttatactcta cgcctcgctg tcgatcgcct tgcgcctgga cgagttccgt
     7441 ccgctggtgc gcatggtcac cgggcgattc ggccggcggg cgtccacgcg caacgggtcg
     7501 caggaagggt cgggatcatg agcaccgcgg cgatcgaacc gcgccagcgc ggcggcatca
     7561 tcaccggatg gctcgtccgg ctggcggtct ctttgctgct gctcggcctg gccttcttcg
     7621 aagccggcgc cgtgatcgtg gcccgcgtcg gagtggacgg caccgctcag accgcggccc
     7681 gcgaggcggc gctcgtctac ggcaacagcc gcgacccgca ggccgccgag gccgaggccg
     7741 ccaaagccgc gcggcagggc ggcgcgaggc tcgtcgagtt cacgatctca acggaaggga
     7801 aacaagtaac cgtgaccctg gaaaggacgg ccaagacctt cgtgatccac aagatcggac
     7861 cgctcgagaa gttcgcgacg acccgcgcct ccgacaccgc cgacgtcagg tagcgcatgg
     7921 acgtgcgcaa ggcaggccca tccgagcgtg acgccttcaa cggcttcgtg tcggcgatgc
     7981 cgtcgggcga tctgctccag tcgtgggagt ggggcgagat caaacgggcc agcggctggg
     8041 cgccggcccg ctatctggtg atggacggcg gccgcatcgt ggcggccgcg agcgtgctgc
     8101 gcacccggcc gctcaagggc gcgccgccgc tgctctacgc cccgcgcggc ccggtgttcc
     8161 ggcccgaggc cgccggcgcg gtccgcgccc tggccgagca gatccgccgc gacgccggca
     8221 gcgccttcgt gctcaagtgc gaccccgcga tcgaggccgg ctcggcggag gcgggcgcgc
     8281 tgcgcgaggc cggcttccgg cccgcgggcg gcgcaggctt cggcggtgtc cagcccaagg
     8341 cggtcatggt gctcgacatc ggccgcgacg aggacgcgat cctggaaggc ttcaagtcga
     8401 agtggcgcta caacatccgg ctggccgagc ggaagggcgt cacggtcgag caggccggac
     8461 gggaagacct tcccgccttc tacgaccttc tgctgatcac cgccaaacgc gacggattct
     8521 tcgtgcgcgg ccgggcctac ttcgagaccc tcttcgacac gctcgagccg cgcgacatgc
     8581 tttcgctgtg gctggcccgc tacgaagcag cgccggtcgc gggtgcgatc tgcttcgcgt
     8641 tcggcaaccg cgtcacctac gtctacggcg cgtcgtcgaa cgagcatcgc aaggtgatgc
     8701 ccaaccatct catgcaatgg acgatgatcc agtgggcgcg caagcgcggc gccacgctct
     8761 acgacttccg cggcgtctcg cccgtccgcg acggcaagcc ggtcgaggag cacatcgccg
     8821 gtctgaaccg ctttaaggaa ggcttcggcg cccgctacgt tgagtacacc ggcgatctgg
     8881 acctgccgct gcgcccgctc gtgtggcgcg cgtgggtcgc gggcgcgccg gccgcgatga
     8941 agctgtacaa gaagatcaag ggaggcgccg gagcggcggc ggattgatgc gcccggaggc
     9001 ggacgcaacg acctgggtcg ccgtgtcggc gggagccctc gaacggaact atcgcgccgt
     9061 cgcgaactac gcgggcacca ccgtgtgcgc ggtcgtgaag gccaacgcct acgggcacgg
     9121 gctcgccgag gcggcccgca ccttcgagag cgccggcgcg cgatggctcg cggtcacgcg
     9181 ggtcgaggaa gcacgcacgc tgcggggggc ggggatcgag tcccacatcc tgatcctgac
     9241 gccgccgccg acggcggggc tcgccgccga ggccgtctcg ctcgacgccg cgctgaccgc
     9301 gagcgacgcc gcggacgtcg accggtacgc ggcagccgcg cgggacgcgg gccggccggc
     9361 gcgcgtgcat ctgaagatcg acacgggcat gggccggctc ggcgttccgg tcagcgaggc
     9421 ggccgcagtc gcggaacgca tcgcggccga ggagcaggtc tcgctcgacg gggtcttcac
     9481 gcacttcgcc ggcgccgccg gtccctccgg cgccgcccag ctctcccgct tccgggccgt
     9541 ccgcgaggcg ctcggcaaac acgcggccag agcgatcgtg cacgcctcca acagcgccgc
     9601 gctgctcaag cttccgggcg cccgcttcga catggtccgg atcggcacgc tgctgtacgg
     9661 ccagaacccg ccgggcgcga cggcgccctt ttcactcgag gagaccttcc agtggtacgc
     9721 ccgtgtcgtc gcggtccgca cggttccggc cggcacgaag gtcggctacg gcggcgagtg
     9781 gacggccaag aagccggtcc ggctcgccac gatcccgatc ggctgggggg acgggttcac
     9841 ggccgagccg catgcgcgca gcgagacgct tgccgccgtc gcgaccgggg ccgcgaaagc
     9901 gatcgcggtc gcggcgggtg cgcgaccgac cccgcgcgtc gtattgttcg gctcgcagaa
     9961 ggcgaaggtg gtggggcggg ccggaatgca ggcgatcacg gtgtcgctcg aagggctgcc
    10021 cggcgtcgcg gtgggggacg tcgcgcgcat tccggcgcgg cggctgctcg tcgcgggcca
    10081 tatcgatcga atctacggag cttgagcgac cacgcatgat cgaggagcgc accgaccacg
    10141 agctcatcgc ggcgcacctg ggcggggacc ggcacgcgtt cgaccggatc gtcgagcgct
    10201 acgagaaacg ggtctgggcc gtctgccttc gcatgtgcgg cgatcccgac gacgcgcgcg
    10261 acgccgccca ggacacgttc gtgaccgcgt tccgctcgat cggatcgttt cgcggagacg
    10321 cgcaactgtc gacctggctg caccggatcg cggtcaacgc ctcgctggac gtgctgcgca
    10381 agcgcggccg caacaagtcc cagccggtcg aggacgtccc cgagatcgcc tccgacgagg
    10441 ccggccccga ggagcaggcg gttcgcgcga gccgcgcggc cgcggtccac gccgcgctga
    10501 ccaggctcag cgaggagcat cgcaccgtga tcgtgctcca cgacctgcaa ggtctgcagt
    10561 atcccgaggt cgcggaggcc ctggacgtac cggtcggaac cgtgaaaagc cggctgcatc
    10621 gggcccgcac cgagatggct cgcttgctcg gtcatctgaa agacgatgga accgaagggc
    10681 ccgcatcgcc ctctaacgtg tcgaggaacc ggcgatgacg acccaccccg aacacgagca
    10741 gctatcggcg tacgccgacg gcgagctcgg cgcgaccgaa cgcgtcgcgg tcgaggcgca
    10801 cctggcggcg tgcggcaagg cgcgcgagat gctggccgcg atccgcgcga ccctggcgga
    10861 cttcgccgcc ttggacgagg tcgagatgga cccgcaggtc ggcttcgcga tccgcagcgc
    10921 gctggcgcgc gagcgcaagc tgtcctcgcg gacgcgacgc tacgcctggg cgggcggcgc
    10981 cgccgctgcg gcgctggtgg gcatcctcag cttcgcgatc ctgggtggcg ggccgaaccc
    11041 ggctcgggac gcgagcgggg cgggattggg tggcggccct cagctcatgg ccatggagga
    11101 cctcgacgag gacagtgccc tgcgggtcct cgagacctat gttcaggaga actacgcctt
    11161 cccgggggct cggggcgaca tgggcggaga cgacgcggcc gccgagaccg ccgtgctgac
    11221 ccacgcgccc ccggcgcctg agatacaggc ctattcggct gccgacgcga cgaccttcgc
    11281 cgattgcatc cgcacgatcg agcgcgagtc cgactcggct cgcctggttc gctcgatcgc
    11341 ggcgacctat aagggcgagc ccgtctatgt gctgatcttc gagattcccg cggagcggcc
    11401 cgaccgggtc gagatgtgga tcacggcccg cgactcctgc gaaacccgct acttcagcca
    11461 ggccaacgtg aaccgctagc gaacctgcat ctttccggac tcgtccgtcc ccggaaattc
    11521 gcaacccggt ccgcggcgtt agaatcggcg tccgaaatct ccgtggaagg acccttcatg
    11581 acgacgcccg aaaacgtgat catcatcgga tccggcccgg ccggcctgac ggcggcgctg
    11641 tacgcggccc gcgcgcagct cgacccgttg gtgatcgaag gcgccgcccc gctcggccag
    11701 ctgatgctca ccaccgaggt cgagaactac ccgggctttc ccgag
//
LOCUS       DATLFO010000261         6432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_15300, whole genome shotgun sequence.
ACCESSION   DATLFO010000261 DATLFO010000000
VERSION     DATLFO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15300"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..991
                     /locus_tag="VM841_10715"
     CDS             116..991
                     /locus_tag="VM841_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90692.1"
                     /translation="MPGIGARDAFHLTTSALRVAQTGETPGDALPQNESFPPAGIGSR
                     PENSPVAKGAGMKRIYWLAGIVALVAAGTQAGPSAAATGAWHCFTKIGPNDILWCQWD
                     PADTTTPEGHFELALPGSPPATSDSGTAIAGGSRYWRLPIPEWMKSLPQTPGIRFTPS
                     GGEWVGPFDVRCTEHSLRGVYVKTVCEVSDGSGGYSYSARDEAPDPDQHAIRLGTLEE
                     TPERRARAERAAALGVGTWEVHSAATAVRSPSGVGRVAGMTVFASIVIFAGATFIRRW
                     GRAKRNRPAELEKAA"
     gene            1334..1456
                     /locus_tag="VM841_10720"
     CDS             1334..1456
                     /locus_tag="VM841_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVL90693.1"
                     /translation="MAAASLTPLARDSTVSRLHAVVERIGAEWSIRDLGSQNGR"
     gene            complement(1470..2222)
                     /locus_tag="VM841_10725"
     CDS             complement(1470..2222)
                     /locus_tag="VM841_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVL90694.1"
                     /translation="MIDSHAHLDMLGDADAAMARARDAGVEAVVAIGVDMTSSRWAAA
                     YAAGHDDVWATVGSHPHDAKDWSVEFADLVRELAAQPRVVGISEAGLDYYYDNSPRDR
                     QQQVFREHIRLAHEAGKTLVIHTRDAWDDTFAILTDEGVPERLVFHCWTGGPAEAERA
                     VEIGAVLSISGIVTFNNAQPIRDAVAITPVERLIVETDSPFLTPVPHRGTKNEPAYVT
                     HVAEAIAQIKGMTMEEFEAATVANTRRVFATD"
     gene            complement(2219..3754)
                     /gene="metG"
                     /locus_tag="VM841_10730"
     CDS             complement(2219..3754)
                     /gene="metG"
                     /locus_tag="VM841_10730"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HVL90695.1"
                     /translation="MGETFYITTPIYYVNDVPHIGHAYTTVAADVAARYHRLKGEDTY
                     FLTGTDEHGGKVAATAAEKGMEPQAWTDQISQRFREVWPRLDISNDDFIRTTEERHIK
                     PVQAFLQHLYDKGEIYEGEYEGPYCQSCEAFYKESELVDGGCPVHKRPVERLKERNYF
                     FRLSAHAGWLLNEYYAQNPCPVQPDSRLNECVSFIKSGLQDVSISRPSLEWGIPLPWN
                     PEHVIYVWIEALQNYITALGYPDGDKFKRYWPAVHLVGKEIVRFHAVIWPAMLHAAGV
                     EPPKMIFGHGWLLVGGEKMSKTNLTGIHPFELVDAFGVDAYRYYFLREVAFGQDGNFS
                     WESMEARYTSELANGIGNLASRVVAMIGAYFDAAVPAPSAPETDQDATLREVCEASVR
                     DYTTAMDALAFHQALEAVDKIVRRANGYIVETAPWVIAKDPTQRDRLARVLFSSAETL
                     RILAVLLSPFMPGACERLWGMLGSSSPLSHQVLPAAGEWGGLRPGSATHKGESLFPRL
                     EQK"
     gene            3829..4071
                     /locus_tag="VM841_10735"
     CDS             3829..4071
                     /locus_tag="VM841_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HVL90696.1"
                     /translation="MRTTGVSRKVDDLGRIVLPAELRKSFDIREGDHVEISVDDDKII
                     LSKRRNTCTFCGQLDSLRSFREHQVCSSCLAELKRF"
     gene            complement(4068..4907)
                     /gene="rsmI"
                     /locus_tag="VM841_10740"
     CDS             complement(4068..4907)
                     /gene="rsmI"
                     /locus_tag="VM841_10740"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HVL90697.1"
                     /translation="MTGSLIIVATPIGNLDDLSPRAAKALAEADLIACEDTRVTRKLL
                     TRVAGSTPMMSYRSANEAQKAAELVARIEAGETVALVTDAGTPGVSDPGHLLVDACAQ
                     RGIPINVVPGPSAAVAALVASGLPTARFVFEGFLPHTRTARRKRLEALAREERTLVLF
                     EAPHRLKTTLADISDVLGDRRIAMAREMTKIHEEFFRSTVSGLQARVGERGVKGEVTL
                     VIEGAPDHGEEKQQFDDADLVEMVRARIGAGASRRDAIDFVADETGLPRRRVYQATID
                     AKV"
     gene            4979..>6432
                     /locus_tag="VM841_10745"
     CDS             4979..>6432
                     /locus_tag="VM841_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HVL90698.1"
                     /translation="METDRSPGVAPRIRGLGFLLGAAVTLFAGIIRFVRLNVPKSLIP
                     LDEFYYPVNSLGLLRHGSDFQLAEGAMLPGVCSQIALEPAFAVHPPVGKWMIGAGMRL
                     FGCNAFGWRVAAALIGTLTVLLVYLIGRRLFRSPWIAAFGALLLAVDGLFFVQSRIAM
                     LDVFLAFFIVAAVWTLIEDRARVRPGHSGWRPWRLGSGVMIGLGIATKWSAAFVVPFL
                     IVLALVWELDRRRRARAIGEEMMAASAGIIPAEGGSGQAPPAPSPEPAYPGEGAIDEP
                     AGTPDAADAPPGDQGAPLPPWDTTASVPGPAYGPLPDPVIAPARGSSVWMTIVRLAGA
                     FLLIPLFVYVLSYIPWLAGEDTRYKPPRCAEQDLIEFDGTISPVNSAIWNWDVWPNQW
                     QEYVCYQREIWDFHKNLKTIDKDGKPTHPYLSRAWSWPWMGRPTSHYFDSEGFDQPCE
                     QPPASAAARYKAAQVTQTAGTATTDPTTPTPSPSP"
BASE COUNT          964 a         2211 c         2179 g         1078 t
ORIGIN      
        1 gcatcaaccg caaccattcc gagttcgtcg ccggccaggt cgtggcctgg cgtgacttcg
       61 tcaacggagc gccggggcgt cgtgcgagcg agccgtggcg agcggatccc gtgccgtgcc
      121 cggcatcggc gcacgcgacg ccttccatct gacgacatcc gccctgcggg tcgcgcagac
      181 cggagagacc ccgggcgacg ccctccccca gaatgagagc ttcccgccag cagggattgg
      241 ctcgcgtccc gagaattccc ccgtagcgaa aggggcgggg atgaaacgaa tctactggct
      301 ggccggaatc gtggctctgg tcgccgccgg cacacaggcc ggaccatcgg cggcagcgac
      361 cggagcgtgg cactgcttca cgaagatcgg cccgaatgac atcctgtggt gccagtggga
      421 cccggccgac accaccaccc cggaaggcca tttcgaactc gccctgcccg gctcgccgcc
      481 ggcaaccagc gacagcggaa ctgccatcgc cggcggatcg aggtactggc ggcttcccat
      541 ccccgaatgg atgaagtcat tgccccagac cccgggcatc cgcttcacac catccggtgg
      601 tgaatgggtc ggcccattcg acgtgcgatg caccgagcac tcgctgcgcg gcgtctacgt
      661 gaagacggtc tgtgaggtga gcgacgggtc gggcggatac tcatactcgg cgcgagacga
      721 ggctccggac cccgatcagc atgcgatccg attggggacc ctcgaggaaa cacccgagcg
      781 ccgtgctcgc gccgagcggg ccgctgccct cggcgtcggc acgtgggaag tgcattcggc
      841 cgcgacggcc gtgcggtctc cgagcggggt gggccgagtc gcaggcatga ccgttttcgc
      901 gagcattgtc atattcgccg gagcgacatt cattcgccgg tgggggcggg ccaagcgcaa
      961 ccggcccgcc gaactcgaga aggccgctta ggcatgcgac ggcgcggcag gatccgcatc
     1021 atcgtcgcgg cagtcgtgac cggtgccctg ctcgtcggat gccggtccca agcaggttca
     1081 tcgctggggc ccgtcacagg cgtcgcttcc cccggctcgc ccgagctctc ctctaccggg
     1141 ccggggccga ccccgtctct tgcacctggg ccgcgatcgc tctccgcgga gaaggtgggg
     1201 tattcggtgt ccgtcaccgt caccgatgcc gacggtacgg tcgccgctcg attcgctatg
     1261 aagaagacga agaggcgctc gcgagcggaa acctctggca ggagacacct cccggggtag
     1321 ccgatcgacc cggatggccg ccgcgtcgct tacaccgctc gccagggact cgaccgtgtc
     1381 tcgtctgcac gccgtggtcg agcgaatcgg cgcggagtgg tcgatccgcg atctcggcag
     1441 ccagaacgga cggtgacggt cgctccggcc taatccgtcg cgaacacgcg gcgggtgttc
     1501 gcgacggtcg cggcttcgaa ctcttccatc gtcatgcctt tgatctgtgc gatcgcttcg
     1561 gcgacgtgcg tgacgtaggc aggttcgttt ttcgtgcccc gatgcggcac gggggtcagg
     1621 aaaggcgagt cggtctcgac gatgagccgt tccacgggtg tgatcgcgac cgcgtcgcgg
     1681 atcggctgcg cgttgttgaa ggtaacgatg ccgctgatcg acagcacggc tccgatctcg
     1741 acggcacgct cggcctcggc ggggccgccg gtccagcagt gaaacaccaa tcgctccggc
     1801 acgccttcgt cggtcaggat cgcaaaggtg tcgtcccagg cgtcgcgggt gtggatcacg
     1861 agcgttttgc cggcctcgtg cgccagccgg atgtgttcgc ggaagacctg ctgctgccgg
     1921 tcgcgcggcg agttgtcgta gtagtagtcc agtcccgctt cgctgatccc gaccacacgc
     1981 ggctgcgcgg cgagctcacg caccagatcg gcgaactcga cgctccaatc cttggcgtca
     2041 tgcgggtgcg agccgacggt cgcccacacg tcgtcgtgcc ccgcggcata ggcggcggcc
     2101 cagcggctcg acgtcatgtc cacgccgatg gcgaccaccg cctcgacacc cgcgtcgcgg
     2161 gcgcgcgcca tcgccgcgtc ggcgtcgccg agcatgtcca ggtgcgcgtg gctgtcgatc
     2221 acttctgttc gagccgcggg aacagcgatt cgcccttgtg cgtggccgat cccggccgca
     2281 gcccgcccca ttcgccggcc gcgggcagta cctgatgcga caacggcgac gatgaaccca
     2341 gcatgcccca cagccgctcg cacgcgccgg gcatgaacgg cgacagcagc accgccagga
     2401 tccgcaacgt ctcggccgag ctgaacagca cgcgcgcgag ccggtcgcgc tgcgtgggat
     2461 ctttcgcgat cacccacggc gcggtctcga cgatgtagcc gttcgcgcgg cgcacgatct
     2521 tgtccaccgc ctcgagcgcc tggtggaacg ccagcgcgtc catcgcggtc gtgtagtcgc
     2581 gaaccgacgc ttcgcacacc tcgcgcagcg tcgcgtcctg atcggtctcc ggggccgacg
     2641 gcgcaggcac ggcggcgtcg aagtacgcgc cgatcatcgc caccacgcgc gaggcgaggt
     2701 tgccgatccc gttggcgagc tcgctcgtgt agcgcgcctc catcgattcc cagctgaagt
     2761 tcccgtcctg cccgaaggca acctcacgca ggaagtaata gcggtaggcg tccacgccga
     2821 acgcgtccac gagctcgaac gggtggatgc cggtcaagtt ggtcttgctc atcttctcgc
     2881 cgccgaccag cagccagccg tgaccgaaga tcatcttggg aggctcgacg cccgcggcgt
     2941 gcagcatcgc gggccagatg acggcgtgga agcgaacgat ctctttgccg accaggtgca
     3001 cggcgggcca gtaacgcttg aacttgtcgc cgtcgggata gccgagcgcg gtgatgtagt
     3061 tctgcagcgc ttcgatccac acgtagatga catgctcggg attccacggc agcgggatgc
     3121 cccactccag cgagggccgc gagatcgaca cgtcctgcag gccggacttg atgaacgaca
     3181 cgcactcgtt gagccgcgag tcgggctgca cggggcaggg gttttgcgcg tagtactcgt
     3241 tgagcagcca gccggcgtgc gcggacagcc ggaagaagta gttgcgctcc ttgagccgct
     3301 cgacggggcg cttgtgcacc gggcagccgc cgtcgacaag ctcggattcc ttgtagaacg
     3361 cctcgcacga ctggcagtag gggccttcgt actcgccctc gtagatctcg cccttgtcat
     3421 agaggtgttg caggaacgcc tgcaccggct tgatgtggcg ctcctcggtc gtgcggatga
     3481 aatcgtcgtt gctgatgtcc aggcgcggcc agacctcccg gaagcgctgc gagatctggt
     3541 cggtccaggc ctggggctcc atgccctttt cggctgcggt cgcggccacc ttgcccccgt
     3601 gctcgtcggt tccggtcagg aaataggtgt cctcgccctt gagccggtga tagcgagcgg
     3661 cgacgtcggc cgcgaccgtc gtataggcat gcccgatatg cggcacgtcg ttgacgtagt
     3721 agatcggcgt cgtgatgtag aaggtttcgc ccacgaaagc tcccgacttt tgacctgcgc
     3781 ctgcctgcga gtccaagatt gcagggctga atcatagtgg agggccggat gcgaacgacg
     3841 ggagtgtccc gcaaggttga cgacctcggc cgcatcgtgc tgcccgccga gctgcgcaag
     3901 tccttcgata tccgtgaagg cgaccatgtc gagatcagcg tcgacgacga caagatcatc
     3961 ctgtcgaagc gccggaacac gtgcaccttc tgcggccagc tcgacagcct gcggtcgttc
     4021 cgggagcacc aggtctgcag ctcctgcctg gcggagctga aacgcttcta gaccttcgcg
     4081 tcgatcgtcg cttggtagac gcggcgccgc ggcaggccgg tctcgtcggc gacgaagtcg
     4141 atcgcgtcgc ggcggctcgc gcccgcaccg atgcgcgccc gcaccatctc gaccagatcc
     4201 gcgtcgtcga actgctgctt ctcctcgccg tggtcgggcg caccctcgat caccaacgtg
     4261 acctcgcctt tgacgccccg ctccccgacc cgggcctgca ggccgctgac ggtcgagcgg
     4321 aagaactcct cgtggatctt ggtcatctcc cgcgccatcg cgatgcgccg gtccccgagc
     4381 acatcggaaa tgtcggccag ggtcgtcttg agccggtgcg gggcctcgaa cagcaccagc
     4441 gtccgctctt cgcgcgccag cgcctccagc cgcttgcgcc gcgcggtgcg cgtgtgcggc
     4501 aggaaccctt cgaacacgaa ccgggccgtc ggcagcccgg acgcgaccag cgccgccaca
     4561 gcagcggacg gtcccggcac gacgttgatc gggatccccc gctgcgcgca cgcgtccacc
     4621 agcagatgcc cgggatccga cacgccgggc gtacccgcgt cggtgaccag cgccacggtc
     4681 tcgccggctt cgatgcgggc caccagctcg gcggccttct gcgcttcgtt ggcgctgcgg
     4741 tacgacatca tcggcgtcga gccggccacg cgcgtgagca gcttgcgggt gacgcgcgtg
     4801 tcctcgcagg cgatcagatc ggcctccgcc agggccttgg ccgcgcgggg cgaaaggtcg
     4861 tcgagattgc cgatcggggt tgcgacgatg atgagcgagc cggtcatggc ctttctatac
     4921 acgcccccgg cccgcggcac ccggttcggg ccggggtccg ttcgtatact gctgccgcgt
     4981 ggagaccgac cgcagccccg gcgttgcgcc ccgaatccgg gggctcggct ttcttttggg
     5041 cgcggcggtc acgctgttcg cgggcatcat tcgcttcgtc cggctcaacg ttccgaagtc
     5101 gctgatcccg ctcgacgagt tctactatcc ggtcaactcg ctcggcctgc tgcggcacgg
     5161 ctcggacttc cagctcgccg aaggcgcgat gctgccgggc gtgtgctcgc agatcgcgct
     5221 ggagccggcg ttcgcggtac atccgccggt cggcaagtgg atgatcggcg cgggcatgcg
     5281 tctattcggc tgcaacgcat tcggctggcg cgtcgcggcg gcgctgatcg gcacgctgac
     5341 ggtgctgctg gtctatctga tcggacggcg tctgttccgc tcgccgtgga tcgcagcgtt
     5401 cggcgcgctg ctgctggcgg tggacggcct gttcttcgtg cagtcgcgca tcgcgatgct
     5461 cgacgtgttc ctggcgttct tcatcgtggc ggcggtctgg acgctgatcg aggaccgcgc
     5521 gcgggtccgt ccgggccatt cgggatggcg gccgtggcgt ctcggttcgg gggtgatgat
     5581 cgggctcggc atcgcgacga agtggtcggc cgcgttcgtc gttccgttcc tgatcgtgct
     5641 cgcgttggtg tgggagctcg accgccgcag gcgcgcccgc gcgatcggcg aggagatgat
     5701 ggccgcctcg gcgggcatca tcccggcgga aggcggctcc ggccaggcgc cgccggcgcc
     5761 ttcgccggag ccggcctatc cgggcgaggg cgcgatcgac gaaccggcgg gcacgccgga
     5821 cgccgcggat gcgccgcccg gcgaccaggg cgctcccctg cccccctggg acacgaccgc
     5881 ttcggttccc ggtccggcct acggtccgct gcccgacccg gtgatcgcgc ccgctcgcgg
     5941 ctcctcggtg tggatgacga tcgtgcgtct ggcgggcgcg ttcctgctga tcccgctgtt
     6001 cgtctacgtg ctgagctaca tcccgtggct ggccggcgag gacactcgct acaagccgcc
     6061 gcgctgcgcc gaacaggact tgatcgagtt cgacggcacg atcagcccgg tgaactccgc
     6121 gatctggaac tgggacgtgt ggccgaacca gtggcaggag tacgtctgct atcagcgcga
     6181 gatctgggac ttccacaaga acctcaagac gatcgacaag gacggcaagc cgacccaccc
     6241 gtacctgtcc cgcgcatggt cgtggccgtg gatgggccgg ccgacctcgc actacttcga
     6301 cagcgaaggt tttgatcagc cgtgcgagca gccaccggcg agcgcggccg ctcgctacaa
     6361 ggccgcgcag gtcacgcaaa cggcgggaac ggcgaccacg gaccccacca ccccgacgcc
     6421 ctccccctca cc
//
LOCUS       DATLFO010000262        10084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88291, whole genome shotgun sequence.
ACCESSION   DATLFO010000262 DATLFO010000000
VERSION     DATLFO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88291"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(494..1339)
                     /locus_tag="VM841_10750"
     CDS             complement(494..1339)
                     /locus_tag="VM841_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL90699.1"
                     /translation="MSTEISGLDSLEARRVEPRRPVRAAWTATWPKLAAVAVVVGVWQ
                     SVAWLRIRPSYVLPDPATVLGALGELFSEPDFLQALGVTVQRGAAGFGVAIVIGTLLG
                     IAVSRSSILRRTFGSLITGLQSMPSIAWFPFAILIFKLSEGAIMFVVVLGAAPAIANG
                     LITGVDHIPPLLLRAGRVLGARGLSAYRHVILPAAFPEYLAGMKQGWAFAWRSLMAGE
                     LLVIIESRPSIGAKLQFARDLSDAPGLLALMIIVLLIGMVVDGLFFGTLERSLRNRRG
                     LNDAR"
     gene            complement(1350..2162)
                     /locus_tag="VM841_10755"
     CDS             complement(1350..2162)
                     /locus_tag="VM841_10755"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL90700.1"
                     /translation="MRLHPPRIGAGPAATRAPGGTAGPAIAIRDVTKTYADGRSATKA
                     LEGLTLDVAPGEFVCILGASGCGKSTLLNLIAGIYRTSAGTIDVAGRRIALMFQEPAL
                     FPWLTVQHNVEMGMRFRRSVTKERRREEARRLLRLMHLEEWASRRPHELSGGMRQRVA
                     LARALAQEAEILLMDEPFGSLDAITRDLLHEEIERIWLENRLTIVFVTHNVREAVRLG
                     DRVVLLSSRPGRLATQFPVGLGRPRRLEDAALGGIAAEISARLHEEVRRHGR"
     gene            complement(2159..3184)
                     /locus_tag="VM841_10760"
     CDS             complement(2159..3184)
                     /locus_tag="VM841_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90701.1"
                     /translation="MPGGRRLIAAITLLALPACAPASGSGPRPPKVRVGYSPNLTHAP
                     AIAGIERGLFAKEIGERGRLDTRRFNAGPDAVQALFSKALDIAYMGPNPAINAYAQSD
                     GKAIRIIAGAVSGGAGLVVRPGIRSADDLRGATLSSPQLGNTQDVALRTWLESKGLRV
                     TRSGPGDASIRPMPNAQIFEAFRDRQIDGAWVPEPWLARMLVEGGGTLLVDEADLWPN
                     GRFVTTHIVVRTAFLRNHPGLVAAFLRGHLAAIDYLRTGGEPARAIVEAAIKRSTGAV
                     LPAEVMHAAWGTITLTPDPLPHSLRQDAADAHEVGLLDRVRLEGIYDLTILNRLLKER
                     GQEAIAA"
     gene            complement(3484..4191)
                     /locus_tag="VM841_10765"
     CDS             complement(3484..4191)
                     /locus_tag="VM841_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HVL90702.1"
                     /translation="MAGFDWVRSGEFSAALAAVLRGARRDAGMTLRTVQRLSGGYFRP
                     SAVNGYERGHRQISVDRLCHIAELYGTTGDELLRRTLASLGRTEPEEKPPEAGSRSAT
                     LRVFYQRIVEDGDLSAIDELCTGTFTDHEIPGRLQLPHNANGFRTWMRLFHRTFENVT
                     IDVEDMMVVGDQAVVRIHLSLTPRQGGARQGQATRISVAISEWMRFSGERLSERWGID
                     GTWLLDQAGISAGSERA"
     gene            4454..5143
                     /locus_tag="VM841_10770"
     CDS             4454..5143
                     /locus_tag="VM841_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90703.1"
                     /translation="MAESSLVRETIDLEDHAAVRVRVLVVSRRLLFAETVQWALDAAG
                     LEVVAAPDVDAAVAFARSVHPDVVVMDVNSTDGDAVAAERALARADAPCTVLVIGDPP
                     AGSAHTGNGVREILPGTIGLDGLAARIRAAASGEPVVVRTGDRRVCLSPEERHAQMVA
                     DSLTGRERQILQLMVSGASGRVIADHLGLSPNTVRTHAQNVLTKLQVGSRLAAVAFAV
                     EHGIVSIPADD"
     gene            5304..5984
                     /locus_tag="VM841_10775"
     CDS             5304..5984
                     /locus_tag="VM841_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90704.1"
                     /translation="MRILLGEPPGLFRDSIRALLREVGSVDGSSPQIIEASTVEEAAR
                     AAHQRPEVAILAASLPPEGGLRAAAAISRVVPGCRIVVLSDGHDRASLIRAVQAGATG
                     FVARVGPLSDLIAAFVAATRGEAYVPAPMLEGILRKAAGHLPSADDPAARIERLTERE
                     REVLAGLALGDGSAALGERLGISPETARTHVQNVLSKLGVHSRLEAVALARRTGLLEL
                     WQVGDQPS"
     gene            5981..6208
                     /locus_tag="VM841_10780"
     CDS             5981..6208
                     /locus_tag="VM841_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL90705.1"
                     /translation="MIVTPVTPRPRLLTPRERQVLVMTADGLTAKEIAARLAISRKTV
                     EQHRGRILLKLGVPNQAAAVRLMERAGGPVT"
     gene            6205..7989
                     /locus_tag="VM841_10785"
     CDS             6205..7989
                     /locus_tag="VM841_10785"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HVL90706.1"
                     /translation="MTLYDLERRAAHGGPLKPLLAGEERIRELRLASRDWSSWNLTAR
                     QLCDVELMLAGAFSPLDGFMTYEEYEGVLESMRLPSGVVWPIPVVLDVDEDTMRAVTR
                     HRALALRDPEGTMVAALHVEDVWQADIGDEAAMVYGTRDPSHPGVAALLNRPLFRIGG
                     RVEGIALPPHYDFRDLRATPLELRERFRTLGWDAVVAFQVRNPMHLAHYELTKRAAEE
                     ADGGLLIHPVVGMTKPGDLDYYTRVRCYRALMPRYRRGSAALSLLPLAMRMAGPREAL
                     WHAIIRRNYGCTHFIVGRDHAGPGVDAAGRPFYDPYAAQELVARYEPEIGIRMVPFRE
                     IVYVEELGRYLAEDEVPDGAARLSISGTQLRRMLANGEDIPEWFSPPEVVRELRRTHP
                     PRARSGLTLFLTGLSGAGKSTIAAALAARLLEDGSRRVTVLDGDLVRKNLSSELGFSH
                     EHRDRNIRRIGFVAAEIAKHGGIAICAPIAPYDETRKQVREECSAAGSFVLVYVRAPL
                     SVCEARDRKGLYAKARAGIIQSFTGITDAYEEPADADLIIDTEHASVEESVDRIMELL
                     RREGYLPSSDATKRDALVARPASPPSGS"
     gene            complement(7986..9635)
                     /locus_tag="VM841_10790"
     CDS             complement(7986..9635)
                     /locus_tag="VM841_10790"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HVL90707.1"
                     /translation="MLYPNARCGGPGTAGAPRARRFARHTISVIVLAALMLSGLALTS
                     AGTAAAGPGASAAECLARSGVTKLGGTYSSQYKVASPGADRTFDARTATFTAYPFSSK
                     YPFTLGSNNPGVRMCVVGGTVIGQQSRSLTWDQMKNQHDGDGLRMAGNDWYVADGLRV
                     DNVEDGFAPRGTAGKYPMDGDGFVLRNAYMKYIRDDCVENDDIAGGLIEDSLFDGCNT
                     GISERPTQGTPAWNHPAPPGETLTVDRVLLRLQAMPGPRGTNDPSVLGHGQLFKWSSV
                     ANKLVVRDSIFLVEKKPNGGDASFPSGTVAKNVTVVWLGGGSYPGKIPSGVKITTDRS
                     VWDKARDHWLARHGCTSFTSCSKLHNPDPYGEPSPAPTTTSSPASSADPISSPAPVAS
                     PAVQKSFVATADATIRKGYPKKNYGKSVALALDSSPEGDSLLKFTVGDLGGKKVSAAW
                     IVLWCTDGGPDGGGTFSMTGSSWSESTVTWANAPSMGAMVGTLGPVVAGKAYLVDVTS
                     VVAGAGTYSFRASLESPKGARYASKESTTASQRPVLVVELV"
     gene            9842..>10084
                     /locus_tag="VM841_10795"
     CDS             9842..>10084
                     /locus_tag="VM841_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90708.1"
                     /translation="MAKRINNQMGRIERVVAAVAVLAVLTGLAALSPALAATTYYVDK
                     ANASCTDSGSGSVAQPFCSIGRGAEVAQAGDSVLVGT"
BASE COUNT         1558 a         3378 c         3567 g         1581 t
ORIGIN      
        1 ctgctcgcac tcatgatcat cgtgctgctg atcggcatgg tggtggacgg gctgttcttc
       61 ggcacactcg agcgttcgct ccgcaatcgc cgcggccgtg agattcggct cgggtgaggg
      121 cgtcgcgtta tccgcggctc cgacggcgag aggctcttca gcggcggcag ggtccgcgga
      181 cggtgccggc gacgcgacga cctcgcccgt cgttgcggtg ccgcgctcgc cggcgacggc
      241 gggaacgcct ccggctgcgg cgatcgcggc ggagatcccc aggatgacga gcacgcggcg
      301 aacctgccgc gtggagcggc gaacgagcga gcgtggattc gatcctgcca tgtcttcacc
      361 tcgtggacgg atcgatcttc accgaccgtc cgcgagcagt gaatagcctc ttcacgtcat
      421 gatttgcggc ctctcgcact agttgccgca atccgccgta ggcgcgttgg ttcgaacgtc
      481 gtagcggcac gggctatcgg gcgtcgttga ggccgcggcg attgcggagc gaacgctcga
      541 gtgtgccgaa gaacagcccg tccaccacca tgccgatcag cagcacgatg atcatgagtg
      601 cgagcaggcc gggcgcgtcg gagaggtcgc gcgcgaactg gagcttcgct ccgatggagg
      661 gacgcgattc gatgatgacg aggagctctc ccgccatcag cgatcgccac gcgaacgccc
      721 agccctgctt catgcccgcg agatactcgg gaaacgccgc cggaaggatg acgtgacggt
      781 acgccgagag tccgcgcgcc ccgagcacgc ggccggcccg cagcagcagc ggcggaatgt
      841 gatcgacgcc ggtaatcagg ccgttcgcga tcgcgggcgc ggccccgagc acgacgacga
      901 acatgatcgc gccctcgctc agcttgaaga tcaggatcgc gaacggaaac catgcgatcg
      961 acggcatcga ctgcagcccg gtgatcagcg acccgaacgt gcgacgcagg atggagcttc
     1021 gcgaaaccgc gatgccgagc agcgttccga tcacgatcgc gacaccgaag ccggccgcgc
     1081 cccgctgcac ggtgacgccg agcgcttgca ggaagtccgg ctcggaaaac agctcgccca
     1141 gcgcgccgag aacggtcgcc ggatcgggga gcacgtagga cggccggatc cgaagccacg
     1201 cgacgctctg ccacacgccg acgacgacgg cgacggcggc gagtttgggc catgtggccg
     1261 tccacgccgc ccgaacggga cggcgcggct cgacgcggcg ggcctcgagc gagtccaggc
     1321 cgctgatctc ggtactcaag atgtccgatt cagcggccat ggcgacggac ctcttcgtgc
     1381 aggcgcgcgg agatctcggc ggcgatgcct ccgagggcgg cgtcctcgag ccgccgggga
     1441 cgtcccagcc cgacggggaa ctgagtggcc aggcgtccgg gccggctcga cagcagcacg
     1501 acccggtcgc cgaggcgcac ggcctcgcgc acgttgtgcg taacgaacac gatcgtgagc
     1561 ctattctcga gccaaatccg ctcgatctct tcgtgcagca ggtcccgcgt gatggcatcg
     1621 agcgagccga acggctcgtc catcagcaag atctccgcct cttgcgcgag cgcccgcgcg
     1681 agcgcgaccc gctggcgcat gcctccggac agctcgtgcg ggcggcgaga ggcccactcc
     1741 tcgagatgca tcagccgcag cagcctgcgt gcttcctcgc gccgtcgctc cttcgtgacg
     1801 gaacggcgaa agcgcatgcc catctcgacg ttgtgctgca cggtgagcca ggggaacagc
     1861 gcgggctcct ggaacatcag cgcgatccgc cggcccgcga cgtcgatcgt gccggcggag
     1921 gtgcggtaga tgccggcgat caggttcagc agggtgctct tgccgcatcc ggaggccccg
     1981 aggatgcaga cgaactcgcc gggcgcgacg tcgagggtca gcccttcgag cgctttggtg
     2041 gcgctgcggc cgtcggcata ggtcttggtg acgtcgcgga tcgcgatcgc gggaccggcc
     2101 gtgccgccgg gcgcccgggt cgcggccggg cctgcgccga tgcgcggagg atggagtctc
     2161 atgcggcgat cgcctcctgg ccgcgttctt tgaggagccg gttgaggatg gtcaggtcgt
     2221 agatgccctc gaggcgcacc cggtcgagca ggcccacctc gtgcgcgtcg gcagcgtctt
     2281 gcctgagcga atgagggagc ggatccgggg tgagggtgat ggtgccccac gcggcgtgca
     2341 taacctcggc gggcaatacc gcgcccgtcg aacgcttgat cgctgcttcc acgattgcgc
     2401 gcgccggctc gcctccggtg cgcagatagt cgatcgccgc gaggtggccg cgaaggaacg
     2461 ccgcgaccag gccgggatga ttccggagaa acgcggttcg aaccacgatg tgcgtggtga
     2521 cgaaccggcc gttcggccac aggtcggctt cgtccaccag caacgtgccg cccccctcga
     2581 ccagcatgcg cgccagccac ggctccggga cccaggcccc gtcgatctgg cggtcgcgga
     2641 acgcctcgaa gatttgcgcg ttgggcatcg gacggattga tgcgtcgccc ggtccgcttc
     2701 gcgtgacgcg caggcccttg ctttcgagcc aggtgcggag cgcgacgtcc tgggtgttgc
     2761 cgagttgcgg ggacgacaag gtcgcgccgc gcaggtcgtc ggcggatcgg atgccgggcc
     2821 gcacgacgag cccggcgccg cccgagactg cccccgcgat gatgcgaatg gctttgccgt
     2881 cggattgcgc gtaggcgttg atggccgggt tcggtcccat gtatgcgatg tcgagcgctt
     2941 tggagaacag cgcctggacg gcgtcgggtc cggcgttgaa ccgccgtgtg tcgagccggc
     3001 cgcgttctcc gatctccttc gcgaacaggc cgcgctcgat gccggcgatc gcgggggcgt
     3061 gcgtcaggtt cggcgagtag ccgacgcgca ccttcggcgg ccgcggcccg gacccggacg
     3121 cgggggcgca cgccggcagc gcgaggagcg tgatcgctgc gatcagtcgt ctcccgcccg
     3181 gcacgattcc tccctcgcgt cgcgggcccg cggggccgtc ttcgtgccgg tcaaaggaat
     3241 cggggccccc ggcggcatca cggaacgaag cgcgacctcc tcccacaccc tgtggtcgaa
     3301 gaaggtccga accgctcgta cccagggacg agcggttcgg cgcatccggt ttgctctcat
     3361 cggtcctcgg aaggtcgcgg atcttcgatc ctcggtttcg cctgcgaggc taggggccac
     3421 gaaggaacgg tctccagacg cgaacggcgt accggccgtg cgcaaggcac cgtagcgaag
     3481 ggcctaggcc cgctcggacc ccgcgctgat gcccgcctga tcgagcagcc aggttccatc
     3541 gatgccccac cgttccgaaa ggcgctcgcc cgagaagcgc atccattccg agatcgcgac
     3601 gctgattcgc gtcgcttgtc cctgccgcgc gcccccctga cgaggagtca gcgacaggtg
     3661 aatgcgaacg acggcttggt caccgacgac catcatgtcc tcgacgtcga tcgtgacgtt
     3721 ttcgaaggta cgatgaaaca ggcgcatcca cgtccggaag ccgttcgcgt tgtgcgggag
     3781 ctgaagccgc ccgggaatct cgtggtcggt gaaggtgccg gtgcacaact cgtcgatcgc
     3841 cgagaggtct ccgtcttcga cgattcgctg atagaagacg cgcagggtcg ccgagcggct
     3901 gccggcctcc ggaggcttct cttccggctc ggtgcggccg agcgaggcga gcgtgcgacg
     3961 cagcagctcg tcgccggtgg tgccgtaaag ctcggcgatg tggcagagcc ggtcgaccga
     4021 gatctggcgg tgaccgcgtt cgtagccgtt cacggccgac gggcgaaagt atccgccgct
     4081 caatcgctgg accgtcctga gcgtcattcc tgcgtcacgg cgcgcaccgc gaagcacggc
     4141 cgcgagcgca gccgagaact cgccggaacg cacccaatca aaaccggcca ttgatccttt
     4201 tgacctccgg ggacgacgct attgcgtttt ccgctcgggc ggaaccgacg attgaagtac
     4261 ggaagggccg tcgcgcgtcg gggggccagt cgccgaatcg gcctagccgc catcggggtg
     4321 tccgcgaagc gcagggatac gacccgcacg aaccatgtcc gaccgcccat caaaggccgt
     4381 tgacgttcgc gcagcgcggc cgacacaatt gcgctctgct ccttctccga cgattcccag
     4441 agggaggcgc atcatggccg agtcgtccct tgttcgggag acgatcgatc tcgaagacca
     4501 cgccgctgtt cgggttcgcg tgctggtcgt aagccgccga ttgttgttcg ccgagaccgt
     4561 ccaatgggcg ctggatgccg ccgggctcga ggtcgtcgcc gcccccgacg tggacgcggc
     4621 ggtcgcgttc gcccgtagcg tgcatcccga cgtggtggtg atggacgtga acagcacgga
     4681 cggggacgcg gtcgccgccg agcgcgccct cgcccgcgca gacgctccgt gcaccgtgct
     4741 ggtcatcggc gatccgccgg ccggctctgc ccacacaggg aacggcgtgc gtgagatcct
     4801 tcccggcacg atcgggctgg acggcctggc ggcgcggatt cgtgccgcgg cttccggcga
     4861 gcctgtggtc gttcgaaccg gcgaccggcg cgtgtgcttg tcgcccgaag agcgtcacgc
     4921 gcagatggtg gccgactccc tgactggacg cgagcggcag atccttcagc tgatggtgtc
     4981 cggtgcgagc ggacgggtca tcgccgatca tctcggcttg agccccaaca cggtccggac
     5041 gcacgcacag aacgttttga ccaagcttca ggtcgggtcc cggctcgcgg cggtcgcctt
     5101 cgccgtcgag cacgggatcg tgtcgattcc ggccgacgac tgatccgcag gggagagggg
     5161 agcgaatgga cgacggcgat gccggcgcgc gtgccacacc agccggatcg acgttgccgg
     5221 cccccggcta cggcgttcgc ggcatgcgca ctacgacgca cggctctcca cggcccgcgg
     5281 ccgtctccgt accattcgcg gcgatgagga tcctgctggg agagcccccc ggattgttcc
     5341 gggactcgat tcgggcgctt ttgcgtgagg tcgggtccgt ggacggcagc agcccgcaga
     5401 tcatcgaggc atcgaccgtg gaagaagccg cccgtgccgc gcaccaacgg ccggaggttg
     5461 cgatcctcgc cgcttcgctg ccgcccgagg gcggcttgcg ggcagccgcg gcgatctccc
     5521 gggtcgtgcc cggttgccgg atcgtggtgc tgtcggacgg ccacgaccgt gcgtcgctca
     5581 tccgcgcggt ccaggcgggc gcgacggggt tcgtggcgcg ggtcgggccg ctgtcggacc
     5641 tgatcgccgc cttcgtcgcg gcgacgcggg gtgaggcgta cgttcccgct ccgatgctcg
     5701 aagggatcct gcgcaaggcg gccggacatc tcccgtcggc ggacgatccg gcagcgcgca
     5761 tcgaacggct gacggagcgg gagcgagagg tgctcgccgg tctcgcgctc ggcgacggaa
     5821 gcgcggcgct cggcgaacgg ctcggcatca gccccgaaac ggcccggacg cacgtgcaga
     5881 acgtgttgtc caagctcggc gtgcactcgc ggctggaagc agtcgcgctg gcgcgcagaa
     5941 cgggattgct cgagttgtgg caggtgggag accagccctc gtgatcgtca cgccggtaac
     6001 gccccggccc cggcttctga ccccgcggga gcgacaggtg ctcgtgatga ccgccgacgg
     6061 gctgaccgcg aaagagatcg cagcgcgtct ggccatcagc cgcaagaccg tcgagcagca
     6121 tcgcgggcgg attctgctca agctcggcgt tcccaatcag gcggccgcgg tgcggctgat
     6181 ggagcgggcg ggcgggccgg tgacgtgacg ctctacgacc tggaacgccg cgccgcccac
     6241 ggcggtccgc tcaagccgct gctggccggc gaggaacgga tccgtgagct tcgcctggcg
     6301 tcgcgcgact ggtcgtcgtg gaacctcacg gcgcgacagc tgtgcgacgt cgagctgatg
     6361 ctcgccggcg cgttctctcc gctcgacggc ttcatgacct acgaagagta cgaaggtgtg
     6421 ctcgaatcga tgcggcttcc atcgggcgtg gtgtggccga tccccgtcgt gctggacgtc
     6481 gacgaggaca cgatgcgagc cgtcacgcgt catcgggcgc tcgcgttgcg ggaccccgaa
     6541 ggcacgatgg tggccgcgct gcacgtcgag gacgtgtggc aagccgacat cggcgatgag
     6601 gcggcgatgg tctacggaac gcgcgacccg tcgcatcccg gcgtcgccgc gctgttgaac
     6661 cggccgctgt ttcgcatcgg cggacgggtt gaaggaatcg cgttgccgcc gcactacgac
     6721 ttccgggacc tgcgcgcgac tccgctggag cttcgcgagc gcttccggac gctcgggtgg
     6781 gacgcggtcg tcgcattcca ggtgcgcaac ccgatgcacc ttgcgcacta cgagctgacc
     6841 aaacgggccg cggaggaggc ggacggggga ctgctgatcc atccggtggt cggtatgacc
     6901 aagcccggcg acctcgacta ctacacgcgc gttcgctgct accgggcctt gatgccgcgc
     6961 taccggcgcg gcagcgccgc tctgtcgctg ctgccgctcg cgatgcggat ggcggggccg
     7021 cgagaggcgc tctggcacgc gatcatccgg cggaactacg gctgcacgca cttcatcgtc
     7081 gggcgcgatc acgccgggcc cggcgtcgac gccgcgggtc gtcccttcta cgacccgtac
     7141 gccgcgcaag agctcgtcgc gcgctacgag ccggagatcg gcatccgcat ggtgcccttc
     7201 cgggagatcg tctacgtcga ggagctcggc cggtacttgg ccgaagatga ggtgcccgac
     7261 ggggccgcac ggctctcgat cagcggaacc caattgcggc gcatgctcgc gaacggcgag
     7321 gacattccgg agtggttctc gcccccggag gtcgtgcgag agctgcgccg gacccacccg
     7381 ccgcgtgcgc gcagcgggct gacgctgttc ctgaccgggc tgtccggcgc gggcaagtcc
     7441 acgatcgccg cagcgcttgc cgcgcgcctg ctcgaggacg gatcgcggcg ggtgacggtg
     7501 ctggacggag atctggtgcg caagaacttg tcgtccgagc tcggcttctc gcacgagcac
     7561 cgcgaccgca acatccgtcg aatcggcttc gtcgcggccg agatcgccaa gcacggcggc
     7621 atcgcgatct gcgccccgat cgcgccctac gacgagacgc gcaagcaggt acgcgaggag
     7681 tgctcggccg ccggcagctt cgtgctcgtc tacgtgcggg cgccgctgtc ggtgtgcgag
     7741 gcgcgtgacc gaaagggcct gtacgcgaag gcgcgcgcgg gaatcatcca gtcgttcacg
     7801 ggcatcaccg acgcctacga ggagcctgcc gacgcggatc tgatcatcga caccgagcac
     7861 gcgagcgtgg aggagtcggt cgaccgaatc atggagctcc tccggcggga aggctacctt
     7921 ccgtcgtccg acgcgacgaa gcgggacgcg ctcgtcgcgc gccccgcctc gccgccttca
     7981 gggtcctaga ccagctccac gaccaggacg ggccgctgcg aagcagtcgt gctttcctta
     8041 gaggcgtagc gcgcgccctt gggtgactcg agcgatgcgc ggaaggaata cgttccggct
     8101 cccgcgacca ccgaggtgac gtctacgaga taggccttgc ccgcgacgac cgggccgagc
     8161 gtgccgacca tcgctcccat cgacggggcg ttcgcccacg tgaccgtgct ctcgctccag
     8221 ctcgagccgg tcatggagaa ggtgccaccc ccatcgggtc ccccgtccgt gcaccacagc
     8281 acgatccatg cggcggacac cttcttgccg cccaggtcgc cgacggtgaa cttcagcaag
     8341 gaatcgcctt cggggctgga gtccagcgcc agagcgaccg actttccgta gttcttcttc
     8401 gggtagcctt tgcggatcgt cgcgtcggcg gtcgcgacga aggacttttg cacggcgggc
     8461 gaagccaccg gcgccggcga ggagatggga tccgcggacg aggccggcga cgaagtcgtc
     8521 gtcggggccg ggctcggctc gccgtaggga tccgggttgt gcagcttcga gcaggacgtg
     8581 aaggaggtgc acccgtgacg cgccagccag tgatcgcgcg ccttgtccca gaccgagcga
     8641 tcggtcgtga tcttcactcc ggacggaatc ttgccgggat aggagccgcc gccgagccat
     8701 acgacggtca cgttcttcgc aaccgtgccc gaggggaagc tcgcgtcgcc gccgttcggc
     8761 ttcttctcca ccagaaagat cgagtctcgc acgacgagct tgttcgccac cgacgaccac
     8821 ttgaagagct gaccgtggcc gagcacgctc gggtcgttgg tgccgcgcgg gccgggcatc
     8881 gcctgcaggc gaagcagcac gcggtccacg gtcagggtct cgcccggcgg cgccggatgg
     8941 ttccaggccg gcgttccctg ggtgggacgc tcagagatcc cggtgttgca gccgtcgaag
     9001 aggctgtcct cgatgaggcc gcccgcgatg tcgtcgtttt cgacgcagtc gtcgcggatg
     9061 tacttcatgt atgcgttacg cagcacgaag ccgtcgccgt ccatcggata cttgcccgcc
     9121 gtgccgcgcg gggcgaatcc gtcctcgacg ttgtccacgc gcaggccgtc ggccacgtac
     9181 cagtcgttgc cggccatgcg cagcccgtcg ccgtcgtgct ggttcttcat ctgatcccag
     9241 gtcagcgagc gcgactgctg gccgatcacg gtgccgccga cgacgcacat ccgaacgccg
     9301 ggattgttcg atccgagcgt gaacggatac ttcgaggaga acgggtacgc cgtgaacgtc
     9361 gcggtgcgcg cgtcgaaggt cctgtcggct cccggtgagg cgaccttgta ttgcgacgag
     9421 taggttccgc cgagcttggt cacgcccgag cgtgcaagac attccgcggc gctcgcgccg
     9481 gggccggctg cggccgtgcc ggccgatgtg agcgccagac cggacagcat caatgcggcg
     9541 agcacgatca cggagatcgt gtgacgagcg aagcggcggg cgcgtggcgc gccggccgtg
     9601 ccgggccctc cgcaacgggc attcgggtag agcatgtgat cctccaaggt gactaggccg
     9661 aacgaacatc ccttctctcg gccccctgtg ctggcccctt caggctcgcc cgtttggatg
     9721 tcgaacgcgt tttgcggaaa ccaccggccc tggatggcta ttcgacagga atgcgaggga
     9781 cgcaccctcc attcgaggga cggaaaagca cggatcgacg gggcagcagc agcggcgtag
     9841 cgtggccaaa aggatcaaca accagatggg gaggatcgag cgggttgtag ccgccgtcgc
     9901 cgtgctcgcg gttctgacgg gcctggcggc gctttcccct gccctcgccg ccaccaccta
     9961 ttacgtggac aaggcgaacg cgtcatgcac ggattcggga tcgggttccg tcgctcagcc
    10021 gttctgctcg atcgggcggg gcgcggaggt cgcgcaggcg ggcgacagcg tgctcgtggg
    10081 aacc
//
LOCUS       DATLFO010000263        10998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_95731, whole genome shotgun sequence.
ACCESSION   DATLFO010000263 DATLFO010000000
VERSION     DATLFO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95731"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..748)
                     /locus_tag="VM841_10800"
     CDS             complement(161..748)
                     /locus_tag="VM841_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HVL90709.1"
                     /translation="MTCPAHPDRDAVSACAVCGRPVCDLCVGSIDEVDFCPVHFEVEE
                     QRIQARAYGLPPLPTPVTIPELLIGVWAVIGAFAPWMSWYRTIVITEGPARLERMIDE
                     TGWEAGGVAALASLSLLAAGVLLGFMVGVRIIRPRMIPAEPVPGAALAVGATTLVLLL
                     IRVATRHVNIYVGLYLAITSAVLLVALGYRLRRRG"
     gene            complement(790..1830)
                     /locus_tag="VM841_10805"
     CDS             complement(790..1830)
                     /locus_tag="VM841_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90710.1"
                     /translation="MGNSKGGTCTDMGNSERSSVKRRARTARGGYDDGREGPGRVMDA
                     YDLKALFAFREHIASLPREAFLSILTPMPPVTRVALLPGSFNPPTSAHLLLAERALGE
                     RFDAVVFVLARETAGKRRTGLLLEDRLLSLRSIAPRGTAVAVTSASLYCDQAEAAAAA
                     LPGAEISILVGSDKLEQIFDAAWYVDRDAALGRLFGAARLVVAPRGDGADAVTRLLEE
                     RGNGRWAGKVDVLPLHPAVGDLSSTRVRGLLTSGADPSGLMPAPVAELIAELGAFSPA
                     DPDTGADRYALRAGLLDALWQARDWAVASADLAALVRIAADPGGEGGRLREMLRTGAA
                     GARELLAAQRTP"
     gene            1858..2646
                     /locus_tag="VM841_10810"
     CDS             1858..2646
                     /locus_tag="VM841_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90711.1"
                     /translation="MDPWVTVRRVIADKRSGASALALRTAGALPFLSGDRDVIRAARH
                     ILRAHGAMGSLWRVFDAALAGRTALARLQGKIEGEAAAVASAAAGWALPRRNARVLTH
                     SSSSIVLATLEHAGPSRIASVECTASLPGGEGRALAARLRRMGFAARAIHDAALARAC
                     AEANVVLVGADAITPASLINKTGTMPLALTARETGVPCYALSGSTKMVPAWIWNPQRA
                     PAYDATPLEIFDAVVTERGPMRTAGLRRAAARITLDERLEAIAR"
     gene            2643..3455
                     /locus_tag="VM841_10815"
     CDS             2643..3455
                     /locus_tag="VM841_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HVL90712.1"
                     /translation="MTDVEIFSIGDELLRGLVMDTNSHWMAKRVAARGASLRRVAVLP
                     DDPPVVAAELRAAIARRPALILTQGGLGPTDDDRTRECVALATDCELVRNEEAERIVR
                     VRYAALADEGRVADGDMTPSRLRMADLPRGALPLDNQIGGAPGVLLELDGVAIACLPG
                     VPQELWWIWENSLSPHLDRILGPGGFFEITVTLSERDESRIAGVMRGVDERHPLVYVK
                     SRATSFDQPDARVRATLTASGPDGEEARALARRAFDDLRDALAAEGVTIERD"
     gene            complement(3452..3895)
                     /locus_tag="VM841_10820"
     CDS             complement(3452..3895)
                     /locus_tag="VM841_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90713.1"
                     /translation="MARTAGRRSANAFLRQVADELPNWLPPAHRGYASEHFGRCFKVW
                     YDAKPVHFEVQFLRNGRLEIAFHMESDLKTNTEINALLERKQHEIRLALGDEPEFFAH
                     GPKWRALREVWSGGNLRSEEAAVEASARLADYVKAIRPLIPPPGA"
     gene            complement(3897..4367)
                     /locus_tag="VM841_10825"
     CDS             complement(3897..4367)
                     /locus_tag="VM841_10825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL90714.1"
                     /translation="MTREDAGLEGPTGGRMADAVRSQDVIFKDTFPGMLGVKITAVTD
                     AATTGEMQVGPNVRHPGGSAHGGAIAGFGDTLAAWATMARLRDGEGFTTIEFKSNFVS
                     GVTDGLLTGEATVAHRGKRTIVVEVRISAAGKLIAIMIVTQAVLGGRGEISPDA"
     gene            complement(4370..6034)
                     /locus_tag="VM841_10830"
     CDS             complement(4370..6034)
                     /locus_tag="VM841_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVL90715.1"
                     /translation="MTTAREAFFEVARSEGARYLFTNPGTTELPIFDELVDDTGIELI
                     LCLQEGVAMGAADGYAAATGRPSLVNLHIAPGLANGLCGMYNAMWGRSPVVVTAGQQD
                     TRQLVLDPMLAGDLVGMAAPFSKWSYECRRPEEVGVAMRRAFQFAAAPPQGPTFVSIP
                     WDVFDQDADFEVPARSEIDHASPPSKSAIEAAARLLAAAERPVIVAGDAVARAGAVPD
                     LVRLAETCGARVAAEPIAGRLVFPTDHPLWAGTMLPNHAGIRAVLEQADVALLAGVVA
                     FSPLMPAPVSPVPPGVTLIQIDEDPYEIAKTYPVEVGMRGDLRAAAAMIADTVAASLS
                     PAQQGAVGARVARYTAEREAAIAGLQSAIDAARDAKPIKPIVACAEIAGAIEPGTCVV
                     DESVTATLALRGTLTLGEPGSYFFTRGGGLGFGLPASIGVKLARPDQPVVAVVGDGTT
                     LYTPQALWTMAHHDVPVVAVVLNNASYLILKAGMITMAGKAAKAGEFPGMDLTDPPVD
                     FPALAASFGVAAERVEVAADIAPAIRAAVASGKPALIEVVVDGSLG"
     gene            6362..7000
                     /locus_tag="VM841_10835"
     CDS             6362..7000
                     /locus_tag="VM841_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVL90716.1"
                     /translation="MEWSLLERLPPEDARRVLASARRRTFSRNEVIFHEGDPADSILL
                     ISKGRVMVRTTTPLGHTAALSVLGPGTFVGELALLAIPALRSATATAIEPVEAMAIHR
                     DAFAELRARHPAITDTLIQLFADTIRRLNQQLLEALYVPADTRVRRRVIELMGTYGGD
                     TIPLTQEDLAGLAGTTRATVNRVLRDEEKRGTVTLSRGKVIVVDADGLKKRT"
     gene            7080..8414
                     /gene="dacB"
                     /locus_tag="VM841_10840"
     CDS             7080..8414
                     /gene="dacB"
                     /locus_tag="VM841_10840"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HVL90717.1"
                     /translation="MRTRLGVLVALVVALAAAAAAPAHARVPNQTPKRDLENAVSRSL
                     SAPESRGAVAGVLVTRGAATLLDLNGDRALVPASLTKLPTTLAALLRFGPDHRFETIF
                     AGADLRGGTVRGDVALIGGGDPAFSTAAYAVKRYEPDPDDDPPVPVYTTRPATVDVLA
                     EAIVKAGVRRITGSLIVDESRFDAQRTQRGWLARYQTRNSIEVGNLSALAIDEGLGDL
                     EGKTLAPDPAMNAGRALAAALARRGVTVAGGIRHGVAPKQLAPIASVKSPPLREIADY
                     TNKYSVNYPAEMLLKNLGASFGGAGTSAAGIRVIHETLAKIGIPMDGFSITDGSGLSV
                     LNTITPRTIAAVIRYALEDDSEAAKAMRDSLPVAGGPGTLFKRLRLEPVQGNLRGKTG
                     LIRGVRGMAGWVRGLDGSTLVYVTLFNEAASAGRMTTPIDVIAIALARFPYG"
     gene            8436..9554
                     /locus_tag="VM841_10845"
     CDS             8436..9554
                     /locus_tag="VM841_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90718.1"
                     /translation="MITAPARTRERTGHAPRVAVALVSAVLAALAPLPSAADETPDPR
                     VRLTILRTTPAEGITVTATQPAIAHLPPLLTLINDTTALVTIHDPDGIPFLRVGPGGV
                     EANPRSRHLGPAVRPNDPPPPSPGGPEPLWQKHESGNRLRWYEPRAAYDGHGPEDRTR
                     RTTLKLFTIPGTAGETALAIEGAVMWDLPLGSVRASITGVEPPVDGVTAQVHHGLLPA
                     IELRNTSGRTLEISGRDGKPFARIGPGGVELNERSPTYADTRRMPEPPPGEPSWIPHD
                     TDVLIWLEKRAAFDGEVPESIQQRGKVTELGTWTVPARIEGEAIEIRGVVRWIPNPAP
                     LAKRDEAGGGLPWWVAAAAAALVLGLGGVVALGRRRGE"
     gene            9557..10300
                     /locus_tag="VM841_10850"
     CDS             9557..10300
                     /locus_tag="VM841_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90719.1"
                     /translation="MRALLVESDGMSLSAGIVVGPQPRAAVVLCHGIPGSARRDPRDE
                     GYGGLARVVAGRGYAAAWLDFRGVRDSPGDFSAAGWIRDLEAMLDALSRDREAAGLPV
                     VAAGSSAGGAIAIAAAARRPDVAAVATLATPASLRLLADDPATAIARLRNLGIIRDPA
                     FPRDPAAWWDEIDELSSDRHVAAIAPRPLLIAHGDADDVVPYAHAEKLYAAAGYPKEL
                     ARLPGAGHQLRKDERGIACLLDWLDRLKL"
     gene            10445..10663
                     /locus_tag="VM841_10855"
     CDS             10445..10663
                     /locus_tag="VM841_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90720.1"
                     /translation="MDEVRTPCSHRGGPEVAVSTVPSGKDETVQFCICTQCRRFWLER
                     DGWLLSRREAVAVLRSWPMDKAVDVLAR"
BASE COUNT         1590 a         3982 c         3918 g         1508 t
ORIGIN      
        1 gcgttgctcc ggctgatcac cgagacgagc ttcaccaagt tcggcatcta cctggccgtc
       61 atcctgggcg cggtgctcgc gtatgcgggc tggatgaaga acaaggccgc cgcctgatcg
      121 tcacgcggat aacgaaaaag ccccggcggg ggctttttcg ttatccgcgc cggcgcagcc
      181 ggtacccgag cgccacgagc agcaccgcgc tggtgatggc cagatacagg ccgacgtaga
      241 tgttgacgtg gcgggttgcc acgcggatca gcagcagcac cagcgtggtg gcgcccacgg
      301 ccagggccgc gccgggcacc ggctcggccg ggatcatgcg cgggcgaatg atgcgcaccc
      361 cgaccatgaa gcccagcagc acgcccgcgg ccagcagtga caggctcgcc agcgcggcta
      421 cgccgcccgc ttcccagccg gtctcgtcga tcatgcgctc gagccgggcc ggtccttcgg
      481 tgatcacgat cgtgcggtac cacgacatcc acggcgcgaa ggcgccgatc acggcccata
      541 cgccgatgag cagctccggg atcgtgaccg gggtcggcag cggcggcagc ccgtaggcgc
      601 gcgcctggat gcgctgctcc tcgacctcga agtgcacggg gcagaagtcg acctcgtcga
      661 tcgagccgac gcacagatcg cagacgggac gcccgcagac cgcgcacgcg gagacggcgt
      721 cacgatcagg atgcgcggga caggtcatcg aaggcaagcc taacggcggg gactgccgcc
      781 gcggcgaagc tacggcgttc gctgcgcggc cagcagctcg cgcgcgccgg cggcaccggt
      841 gcgaagcatc tcgcgcagcc gcccgccctc gccgccgggg tccgcggcga tacgcacgag
      901 cgccgcgaga tcggccgagg cgacggccca gtcgcgcgcc tgccacagcg cgtcgagcag
      961 cccggcccgg agcgcgtacc ggtccgcccc ggtgtcggga tcggcgggtg agaatgcgcc
     1021 gagctcggcg atgagctccg cgaccggcgc gggcatcaga ccgctcggat ccgcgccgga
     1081 ggtcaaaagg ccgcgaaccc gcgtcgagga caggtctccg acggccggat ggagcgggag
     1141 cacgtcgacc ttgccggccc accggccgtt gccgcgctcc tcgagcagcc gcgtcaccgc
     1201 gtcggctccg tcgccgcgcg gcgccaccac cagccgggcc gcgccgaaca ggcggcccag
     1261 ggccgcgtcg cggtcgacgt accacgcggc gtcgaagatc tgctcgagct tgtcggatcc
     1321 gaccaggatc gagatctcgg cgccgggcag cgcggcagcg gccgcctcgg cctgatcgca
     1381 gtacagcgac gcggacgtga cggccacggc ggtgccgcgc ggcgcgatgg atcgcaggga
     1441 gagcagccgg tcctccagca gcagccccgt gcgccgcttg cccgcggtct cgcgagccag
     1501 cacgaacacc accgcgtcga aacgctcgcc gagcgcgcgc tccgccagca gcagatgcgc
     1561 cgaagtcggc gggttgaacg agcccggcag cagcgcgacg cgcgtcaccg gaggcatcgg
     1621 tgtcaggatc gacaaaaaag cttcacgcgg cagcgaggcg atgtgctcgc gaaaggcgaa
     1681 tagggccttc aggtcgtacg catccatgac tcttcccggc ccctcccggc cgtcatcgta
     1741 gccgccgcgg gccgtccgcg cccggcgttt gaccgacgat cgctcgctgt tgcccatgtc
     1801 ggtgcaagta ccgcccttgc tgttgcccat gtcggtgcaa gcaccgacaa tcgcggcgtg
     1861 gatccctggg ttacggtccg gcgcgtgatc gcggacaagc gatcgggcgc gtccgcgctt
     1921 gctttgcgca cggcgggcgc gctgcccttt ctttccggcg atcgcgatgt gatacgtgcg
     1981 gcgcgacaca tccttcgcgc gcacggagcg atgggctcgc tgtggcgggt cttcgatgcg
     2041 gcgctggcgg gccgcaccgc gctcgcgcgg cttcagggga agatcgaagg cgaagccgct
     2101 gcggtggcgt cggccgcagc cggatgggcg ctgccgcgtc gcaatgcgcg cgtgctgacg
     2161 cactcctcgt cctcgatcgt gctcgccacg ctcgagcacg ccggcccgtc gcgcatcgcg
     2221 agcgtcgagt gcaccgcgtc gctgcccggc ggcgaaggcc gcgcgctcgc cgcgcggctg
     2281 cggcggatgg gcttcgcggc gcgcgcgatc catgacgccg ccctggcacg cgcatgcgcg
     2341 gaagccaacg tggtgctggt cggcgccgac gcgatcacgc ccgcgtcgct gatcaacaag
     2401 accggcacga tgccgctggc gctcaccgcg cgcgagaccg gcgtgccctg ctacgccctg
     2461 tccggctcga cgaagatggt gcccgcctgg atttggaatc cgcagcgcgc acccgcctac
     2521 gatgccacgc cgctcgagat cttcgacgcg gtcgtgaccg agcgcgggcc gatgcgaacc
     2581 gcgggcctgc gccgcgccgc ggcacggatc acgctggacg agcggctgga ggcgatcgcg
     2641 cgatgaccga tgtcgagatc ttctcgatcg gcgacgagct gctgcgcggg ctggtgatgg
     2701 acaccaactc gcattggatg gccaagcgag tcgcggcgcg cggcgcgtcg ctgcgccgcg
     2761 tggcggtcct gcccgacgat cccccggtgg tcgccgccga gcttcgcgct gcgatcgcac
     2821 gccgcccggc gctgatcctg acccaaggcg gcctcggccc gaccgacgat gaccgcaccc
     2881 gcgagtgcgt cgcgctggcc acggactgcg agctggttcg caacgaggag gccgagcgga
     2941 tcgtgcgggt gcgctacgcc gcgctcgccg acgagggccg cgtcgccgac ggcgacatga
     3001 cgccgtcgcg gctgcggatg gccgacctgc cgcgcggcgc gctcccactc gacaaccaga
     3061 tcggcggcgc gccgggcgtg ctgctcgagc tcgacggcgt ggcgatcgcc tgcctgcccg
     3121 gcgtgccgca agagctgtgg tggatctggg agaactcgct gtcgccgcat ctggaccgga
     3181 tcctgggtcc cggcggattc ttcgagatca ccgttacgct ctccgagcgc gacgagtcgc
     3241 gcatcgccgg cgtgatgcgc ggcgtggacg agcgtcatcc tctcgtctac gtgaagtccc
     3301 gcgcgacctc gttcgaccag cccgacgcgc gggtccgggc gacgctgacc gcctccgggc
     3361 cggacggcga ggaagcgcgc gcgctcgccc ggcgcgcctt cgacgatctg cgcgacgcgc
     3421 tcgcggccga aggcgtcacg atcgagcgcg attaggcgcc gggcggcggg atcagcgggc
     3481 ggatcgcctt gacgtagtcg gccaggcgtg cgctcgcctc gacggcggcc tcctcgctgc
     3541 gcaggttgcc gccgctccag acctcgcgca gcgcgcgcca cttcgggccg tgcgcgaaaa
     3601 actcgggctc gtcaccgagc gcgagccgga tctcgtgctg cttgcgctcc agcagcgcgt
     3661 tgatctcggt gttggtcttg aggtcggact ccatgtgaaa ggcgatctcg agccggccgt
     3721 tgcgaaggaa ctgaacctcg aagtggacgg gcttggcgtc gtaccagacc ttgaagcacc
     3781 ggccgaaatg ctcgctcgcg tagccgcggt gcgcgggcgg cagccagttg gggagctcgt
     3841 cggcgacctg gcgaaggaac gcgtttgcgc tgcgacggcc ggcggtgcgc gccatgctag
     3901 gcgtcggggc tgatctcgcc gcgaccgccc aggaccgcct gcgtcacgat catgatcgcg
     3961 atcagcttgc cggcggccga gatgcggacc tcgaccacga tggtgcgctt cccgcgatgc
     4021 gcgacggtcg cctcgccggt cagcagcccg tcggtcacgc ccgagacgaa gttcgacttg
     4081 aactcgatcg tggtgaagcc ctcgccgtcc cgcaggcgcg ccatcgtcgc ccacgccgcg
     4141 agcgtgtcgc cgaacccggc gatcgcaccg ccgtgcgcgc tgccgccggg atggcgcacg
     4201 ttcggcccca cctgcatctc gccggtcgtc gccgcatcgg tcaccgcggt gatcttcacg
     4261 ccgagcatgc cggggaaggt gtccttgaag atgacgtcct gcgagcggac cgcgtcggcc
     4321 atgcggccgc cggtcggtcc ctcgagccct gcgtcctcgc gtgtcatcgc tagccgagcg
     4381 atccgtccac gacgacctcg atcagcgcgg gcttgccgga cgcgaccgcc gcgcggatcg
     4441 cgggcgcgat gtcggcggcg acctcgacgc gctcggcggc gacgccgaac gaggccgcga
     4501 gcgccgggaa gtccaccggc gggtcggtca ggtccatgcc cggaaactcg ccggccttcg
     4561 cggccttgcc ggccatcgtg atcatgccgg ccttcaggat cagatacgag gcgttgttga
     4621 gcaccaccgc cacgaccggc acgtcgtggt gcgccatcgt ccacagcgcc tgcggcgtgt
     4681 agagcgtggt gccgtcgccg acgaccgcga cgaccggctg gtcgggccgc gcgagcttga
     4741 cgccgatcga ggcgggcaag ccgaacccga gcccgccgcc gcgcgtgaag aagtacgagc
     4801 cgggctcgcc cagcgtgagc gtgccgcgca gcgcgagcgt cgcggtcacc gactcgtcca
     4861 ccacgcacgt gccgggctcg atcgcgccgg cgatctcggc gcacgccacg atcggcttga
     4921 tgggcttggc atcgcgggcc gcgtcgatcg cggactgcag gccggcgatg gcggcttcgc
     4981 gctcggccgt gtagcgcgcg acgcgcgcgc cgaccgcgcc ctgctgcgcg ggagagagcg
     5041 aggccgcgac cgtgtctgcg atcatcgcgg cggccgcgcg caggtcgccg cgcatgccga
     5101 cctcgaccgg ataggttttg gcgatctcgt agggatcctc gtcgatctgg atgagcgtca
     5161 cgcccggcgg caccggcgag accggcgccg gcatcagcgg cgagaacgcg acgacgccgg
     5221 ccagcaacgc cacgtcggcc tgctcgagca ccgcgcggat gccggcgtgg ttcggcagca
     5281 tcgtgccggc ccacagcgga tgatcggtcg gaaagaccag ccgtcccgcg atcggctcgg
     5341 ccgccacgcg cgcgccgcag gtctcggcca gacgcaccag gtcgggcacg gcccccgccc
     5401 gcgcgaccgc gtcgccggcg acgatcaccg gccgctcggc ggcggccagc agccgcgcgg
     5461 cggcctcgat cgcgctcttg gacggcggcg aggcgtgatc gatctcgctg cgcgcgggaa
     5521 cctcgaagtc ggcgtcctgg tcgaacacgt cccacgggat cgacacgaac gtcgggcctt
     5581 gcggcggcgc ggcggcgaac tggaacgcgc ggcgcatcgc gacgccgacc tcctcgggcc
     5641 ggcggcattc gtaggaccac ttcgaaaacg gcgcggccat gccgaccaga tcgccggcga
     5701 gcatggggtc gagtacgagc tggcgcgtgt cctgctggcc ggccgtcacc accaccggcg
     5761 agcgtcccca catcgcgttg tacatgccgc acagaccgtt cgcgaggccg ggcgcgatgt
     5821 gcaggttcac cagcgacggc cgcccggtcg cggccgcgta gccgtcggcc gcacccatcg
     5881 cgacgccttc ttgcaggcac aggatcaact cgatgccggt gtcgtcgacc agctcgtcga
     5941 agatcggcag ctccgtggtt cccgggttgg tgaacaggta gcgcgcgcct tcgctgcgtg
     6001 cgacctcgaa gaaagcctcg cgtgcggtcg tcatggcccg acgctagtgg cggccgcgcg
     6061 cgagcgtcaa aacgaagaac ggagcgcccg agaaaggaga gggccgggcc gcgagcggga
     6121 tgagggagcc gcccgagacc cggccggagc acctgcgcgt ccaaaccccc cggcgggacg
     6181 cacagttggg aggtgcttct tcaccgatca cgctaccggt ggcccggccg ggcatccgcc
     6241 gcctgcacga cgcgccgagt gtgacgaagg acacacttcg tgcggtaggc tcgacccggc
     6301 gcggcccccg caccgacgct cccgcaccga cgctcccgca ccgacgagga ggaactgctc
     6361 agtggaatgg tcgctgctcg aaaggcttcc ccccgaggat gcgcgacgag tgctcgccag
     6421 cgcgcgccgg cggacgttct cgcgcaacga ggtgatcttc cacgagggcg acccggcgga
     6481 ctcgatcctt ctgatctcga agggacgcgt gatggtccgc accaccacgc cgctcgggca
     6541 caccgcggcg ctgagcgtgc tgggcccggg tacgttcgtc ggcgagctcg cgctgctggc
     6601 gattcccgcg ctgcggtcgg cgacggcgac cgcgatcgag ccggtcgagg cgatggcgat
     6661 ccaccgcgac gcgttcgccg agctgcgggc gcggcatccg gcgatcaccg acacgctcat
     6721 ccagcttttc gccgacacga tccgccggct caaccagcag ttgctcgagg ccttgtacgt
     6781 cccggccgac acgcgcgtac ggcgccgcgt gatcgagctg atgggcacct acggcggcga
     6841 cacgattccg ctgacccaag aggacctcgc cggcctcgcg ggcacgacgc gggccaccgt
     6901 caaccgcgtg ctgcgcgacg aggagaagcg cggaaccgtc acgctctcgc gcggcaaggt
     6961 aatcgtcgtg gacgccgacg ggctcaagaa acggacctga tccccggttg ctttgcgggc
     7021 gatatccgcc gatcgtcccg cttgccaggc gcgccacagc ctttaccatt gacgcaatca
     7081 tgcgaacccg gttgggggtc ctcgtcgcgc tcgtcgttgc cctggcggcg gcggctgctg
     7141 cgcccgcgca tgcgcgcgtc ccgaaccaaa cacccaagcg cgacctcgag aacgcggtgt
     7201 cgcgctcgct gtcggcgccg gagtcgcgcg gggcggtggc cggcgtactg gtgacccgcg
     7261 gcgccgcgac gcttttggac ctcaacggcg accgggcgct ggtgccggca tcgctgacca
     7321 agcttcccac cacgctcgcc gcgctgctgc gcttcggacc ggatcatcga ttcgagacga
     7381 tcttcgcggg cgcggacctg cgcggcggaa cggttcgcgg tgacgtcgcg ttgatcggcg
     7441 gcggcgatcc cgcattcagc acggccgcct acgccgtgaa gcgctacgag cccgaccccg
     7501 acgacgatcc gcccgtgccc gtctacacga cccggcccgc gacggtggac gtgctcgccg
     7561 aggcgatcgt caaggccggg gtgcgccgca tcaccggctc gctgatcgtg gacgagtcgc
     7621 gattcgacgc gcagcgcacg caacgcggat ggctcgcgcg ctatcagacc cgcaacagca
     7681 tcgaggtcgg caacctgtcg gcgctggcca tcgacgaagg gctcggcgat ctggaaggca
     7741 agacgctcgc acccgatccg gcgatgaacg cgggccgcgc tctcgccgcg gcgctcgcgc
     7801 ggcgcggggt gaccgtcgcg ggcgggatcc ggcacggcgt cgcgccgaag cagctcgcgc
     7861 cgatcgcttc ggtgaaatcg ccgccgcttc gcgagatcgc cgactacacc aacaagtaca
     7921 gcgtgaacta tccggccgag atgctgctca agaacctggg cgcctcgttc ggcggcgcgg
     7981 gcacgagcgc ggccgggatt cgcgtgatcc acgagacgct cgcaaagatc ggcatcccca
     8041 tggacggctt ctcgatcacc gacggatcgg ggctgtcggt gctgaacacg atcacgccgc
     8101 gcacgatcgc cgcggtgatc cgctacgcgc tcgaggacga ctcggaggcc gcgaaggcga
     8161 tgcgcgactc gcttccggtc gccggcggtc ccggcacgct gttcaagcgg cttcgtttgg
     8221 agcccgtgca aggcaacctt cgcggaaaga ccggcctgat ccgcggcgtg cggggcatgg
     8281 ccgggtgggt gcgcgggctc gacggctcga cgctcgtcta cgtgacgctg ttcaacgagg
     8341 cggcgagcgc cggccgcatg accacgccga tcgacgtgat cgcgatcgcg ctggcccgct
     8401 tcccctacgg gtagcgcata tcctggcgcc gagccatgat cacagccccc gcgcggacgc
     8461 gcgaacggac cggtcacgcc ccgcgcgtcg cggtggcgct cgtgtcggcg gtcctggccg
     8521 cgctcgcgcc cctgccctcg gccgccgacg agacgccgga cccccgggtt cgcctcacga
     8581 tccttcgcac cacacccgcc gaagggatca ccgtgacggc cacgcagccc gcgatcgcgc
     8641 acctgccgcc tttgctgacc ctgatcaacg acaccacggc gttggtgacc atccacgatc
     8701 cggacgggat cccgttcctt cgcgtcgggc cgggcggcgt cgaggccaac ccgcgatccc
     8761 ggcacctcgg tcccgcggtc cggcccaacg atcctccgcc accgtctccc ggcggacccg
     8821 agccgttgtg gcagaagcat gagagcggca accggctgcg ctggtacgag ccgcgcgccg
     8881 cctacgacgg ccacgggccc gaggaccgga ccaggcgcac gacgctgaag ctgttcacca
     8941 tcccgggcac cgcgggcgag accgcgctcg cgatcgaggg cgcggtcatg tgggacctgc
     9001 cgctcggctc ggtccgcgcc tcgatcaccg gcgtcgagcc acccgtggac ggcgtcaccg
     9061 cgcaggtgca tcacgggctg ctgccggcga tcgagcttcg caacacctcg ggacggacgc
     9121 tggagatcag cggccgcgac ggaaagccgt tcgcgcgcat cggtcccggc ggcgtcgagc
     9181 tcaacgaacg gtcaccgacc tacgcggaca cgcgacggat gcccgagccc ccgcccggcg
     9241 agccgtcctg gattccccac gacaccgacg tgctgatctg gctcgagaag cgcgccgcct
     9301 tcgacggcga ggttcccgag tcgatccagc agcgcggcaa ggtcaccgag ctcggcacct
     9361 ggacggtgcc cgcccgcatc gagggcgagg cgatcgagat ccgcggggtc gtgcgctgga
     9421 tcccgaaccc ggctccgctc gcgaagcgag acgaggccgg cggcggcttg ccgtggtggg
     9481 tcgccgcggc ggccgccgcg ctggtgctcg ggctcggcgg cgtggtcgcg ctcggacggc
     9541 ggcgcggcga atgaggatgc gcgcgctcct cgtcgaatcc gacggcatga gcctgtcggc
     9601 cgggatcgtg gtggggccgc agccgcgtgc cgccgtggtg ctgtgtcacg gcattcccgg
     9661 gtccgcgcgc cgcgacccgc gcgacgaagg ctacggcggc cttgcgcgcg tggtggccgg
     9721 acgcggctac gcggcggcat ggctcgactt ccgcggcgtg cgcgactcgc ccggcgactt
     9781 ctcggccgcg ggctggatcc gggatctgga ggcgatgctc gacgcgctct cgcgcgatcg
     9841 cgaggcggcg ggtcttccgg tggtggcggc cggctcgtcg gcgggcggcg cgatcgcaat
     9901 cgccgcggcg gcgcggcggc ccgacgtcgc ggcggtcgcc acgctcgcta cgcccgcgtc
     9961 gctgcgtctg ctggccgacg atccggcgac ggccatcgcg cggctgcgca acctcggcat
    10021 catccgcgac ccggctttcc cgcgcgatcc cgccgcctgg tgggacgaga tcgacgagct
    10081 gtcgtcagac cgccacgtcg cggcgatcgc gccgcggccg ctgctgatcg cgcacgggga
    10141 cgccgacgac gtggtgccct acgctcacgc cgaaaagctc tacgcggcgg ccggctatcc
    10201 caaggagctc gcccgcctgc cgggcgccgg ccaccagctt cgcaaggacg agcgtggcat
    10261 cgcctgcctg ctcgactggc tcgaccggtt gaagctatag ccaatcggcc cgtatcggac
    10321 gggcaaaagg cccgccccgc cctgatcctg ccggggtgac cacttcacgc gctcaagcgt
    10381 tcacgtatcg cgtccgatac tcccttcgaa ttcccatcgc ctatcggagt caggagagga
    10441 ccgcgtggac gaggtcagaa cgccgtgctc gcatcgcggt ggaccagagg ttgcggtgag
    10501 caccgtgccg tcgggcaagg acgaaacggt tcagttctgc atctgcacgc agtgtcgccg
    10561 cttctggctc gagcgggacg gctggctgct gagccgccgc gaggcggtgg ccgtgctgcg
    10621 gtcctggccg atggacaagg ccgtggacgt gctggcccgc tagagacgtt cgctcggggg
    10681 agagtcacag cccgacgcca accccgtcgc acacacgaga cgccggagcc gcaaccggcg
    10741 tctcgtccat tttcttgggg ctgttctccg gcggttcggg ggtcgaaatt tcatcgtcga
    10801 ccggcagggc tcgaagccct ccggggcgaa gcctttccca aggctcgtcg tgagcctcgc
    10861 ctcgagggga gctctcatgc gaaagctggc catggctctg gcgatcatga cgcttgcgag
    10921 cggaaccgcc gcgcgagccg acgccttcac gagcgcctcg tatttcgaca cctgcaccga
    10981 cgccgtcgtc gaaggcaa
//
LOCUS       DATLFO010000264         4367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_5771, whole genome shotgun sequence.
ACCESSION   DATLFO010000264 DATLFO010000000
VERSION     DATLFO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5771"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1481)
                     /locus_tag="VM841_10860"
     CDS             complement(306..1481)
                     /locus_tag="VM841_10860"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HVL90721.1"
                     /translation="MRSFRGVVAGALALVFALPAGPAVAAGYGPVPNVNGYTITSRQD
                     LSPTLVYVQYRSSNPRRVINVAFLRRGGGQVLRRVLSNDRVAGPNPRVETVSSMCKRV
                     SCRIAVNGDFFSTTTGQPAGGMAIEGEPIRTPPDARHHFFADWSGYVGVHRISMPSKL
                     YATYPNGQKPIYVHSINTARDANRAVLYSRRWGYSTETTASGFELVLRVTNAPLKMDK
                     TMNVTIVGGAARTNTSIPADGLVLSGSGGHATALATLWNDVRRGAVPAAATLMLDSVP
                     DLRTYVGGTPALLQGGKQAFTSDGSSFMTKRDPKTMVGRRWNGDAILAVVDGRQPSWS
                     VGMTMTEAMQFMRSLGATDALNLDGGGSSEFVKNGVVINKPSDGRERPIAAALTIVG"
     gene            complement(1580..2779)
                     /locus_tag="VM841_10865"
     CDS             complement(1580..2779)
                     /locus_tag="VM841_10865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL90722.1"
                     /translation="MQKSFADLGVSKSVSSALAKRNINAPFACQILAIPDAIEGRDVL
                     VKSQTGSGKTLAFSIPIVERLDPADKRPSALVLVPTRELASQVAVELRDVATARGLKV
                     AVAYGGVSIKEQAMKAAKAHILVATPGRLEDLAQRRLISLASIRILVLDEADRMLDMG
                     FQPQVDRLVDRIPGERQTMFFSATLDGEVGRLARSYTNEAILHETESEKQTASDVEHR
                     FVPVAGVEKADRLADLLKEEGGIPTLVFVRTKRGVDRLVDRLGSRGVDAAGMHGDMSQ
                     AARERSLKRFASGRVSALIATDVAARGLDLDSIARVVNYDPPEDDKSYVHRVGRTGRA
                     GRSGIGITFVAPDQQGDVGRMAARLELSDEYTQENLVIPPPRVVYSSRKRGRRSLLGG
                     GQRRRRI"
     gene            3163..3528
                     /locus_tag="VM841_10870"
     CDS             3163..3528
                     /locus_tag="VM841_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90723.1"
                     /translation="MSGRNGRRRLPEGSLIVDFVCDDDSVGVICYGEFDLTTASILDD
                     AISDGAGRAAGRTLVLDLSGLHFCSVAILQRLRRAALLARASATTLEVVEGRVLARLL
                     TVIDPDRRCYEIGERDGRS"
     gene            3551..3970
                     /locus_tag="VM841_10875"
     CDS             3551..3970
                     /locus_tag="VM841_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90724.1"
                     /translation="MARYYVYIDLSPSEGYVLGATLREPWDDGAFRETSLAAAIAGQE
                     ARIYTDEEMWDDPSRRAALAQWFAERDDSVDDIGVARPSLRLLTAPLDTRTIAERSRA
                     AIAEARRAVREASKRREEARQVRERRTPRPTQPERSA"
     gene            3967..4296
                     /locus_tag="VM841_10880"
     CDS             3967..4296
                     /locus_tag="VM841_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL90725.1"
                     /translation="MSFGAQLRDARTAAGMSQADLEQATGIPKSRLSRYENDHILPSV
                     PSLRRLAEALGVTESHLVTPKTDPWTALLTRLRASGLQLRSVKEAESIADSLIATIAE
                     RRRREIY"
BASE COUNT          724 a         1479 c         1478 g          686 t
ORIGIN      
        1 cggccgagca tctcgaccgc ggtcgcgatc agcgcgtcgg cctggcgaag caagtcacac
       61 gccgattcgg catcggacgt ccgcttcaat tgcggttggt cgagcggcca agattcatga
      121 gtgagcatgc gatcaaacta acaaaggggt acgacaacaa cgtctggcat acatgagaag
      181 ttcgtcgggc cgtccggaga ccacgtcaac acgatccaaa catcctcgac cggcacgccc
      241 gccgacagcc aggtatgcag gttcttgggt gtgaggttcc ccaaatccgc ggggccgctg
      301 agatatcagc cgacgatcgt cagggctgcc gcgatgggac gctcgcggcc gtcgctgggc
      361 ttgttgatga ccacaccgtt cttgacgaac tcgctcgaac ccccaccgtc caggttcagc
      421 gcgtcggtcg caccgagcga acgcatgaac tgcatcgcct cggtcatggt catcccgacc
      481 gaccaggacg gctgccggcc gtccacgacg gcaaggatcg catccccgtt ccaccggcgt
      541 cccaccatgg tcttggggtc gcgcttggtc atgaacgacg agccgtcgct ggtaaacgcc
      601 tgcttgccgc cttgcagcag cgcgggcgtg ccgccgacgt aggtgcgcag gtcgggcacc
      661 gagtccagca tcagcgtggc cgcggccggc acggcgcctc ggcgaacgtc gttccacaat
      721 gtcgccagcg cggtcgcgtg acctccgctg cccgacagca ccagaccgtc ggcggggatg
      781 gacgtgttcg tgcgcgctgc gcccccgacg atggtgacgt tcatggtctt gtccatcttc
      841 agcggcgcgt tggtcacgcg cagcaccagc tcgaagccgg acgccgtggt ctcggtcgaa
      901 tagccccagc gacgcgagta cagcacggcc cggttggcgt cccgcgcggt gttgatcgaa
      961 tgcacgtaga tcggcttctg accgttcggg taggtcgcgt acagcttcga cggcatcgag
     1021 atccggtgca cgccgacgta gccgctccag tcggcaaaga agtgatgtcg cgcgtcgggc
     1081 ggcgtgcgga tcggctcgcc ttcgatcgcc atgccgcccg ccggctggcc ggtcgtggtc
     1141 gaaaagaagt ccccgttgac cgcgatccgg cacgagaccc gcttgcacat tgacgaaacc
     1201 gtttcgaccc tcgggttggg cccggcaacc cgatcgttgg acagcaccct acgcaacacc
     1261 tgtcccccgc cgcggcgcag gaaggcgacg ttgatcacgc ggcgtgggtt cgaagagcgg
     1321 tactgcacgt acaccagcgt cggggacaga tcctgccggc tcgtgatcgt gtagccgttg
     1381 acgttcggca ccggaccata gccggccgca accgccggcc ccgcgggcag cgcgaacacc
     1441 agcgcgagcg cgccggcaac caccccacga aagcttcgca tcatcgagat ggattcggcg
     1501 atccgccgaa cccgcttgaa caccgggaca aaacgaagac ggcgcggaca tgttcccgcg
     1561 ccgtccgcat tcaagcgatt tagatgcgcc ggcgacgctg gccgccgccc agcagcgagc
     1621 gacgtccccg cttgcgcgac gagtacacga cgcgcggcgg cgggatgacc aggttctcct
     1681 gcgtgtactc gtcgctcagc tcgagccgtg cggccatgcg gccgacgtcg ccctgctggt
     1741 cgggagccac gaaggtgatg ccgatgccgc tccgtccggc acggccggtg cggccgacgc
     1801 ggtgcacgta gctcttgtcg tcctccggcg ggtcgtagtt cacgacccgg gcgatcgaat
     1861 ccaggtcgag accgcgagcc gccacgtcgg tggcgatcag cgccgacacc cggccggacg
     1921 cgaaccgctt cagcgagcgc tcgcgcgccg cctggctcat gtcgccgtgc atgccggccg
     1981 cgtccacgcc ccggctgccg agccggtcca cgagccggtc gacgccgcgc ttggtgcgaa
     2041 cgaagaccag cgtcgggatg ccgccctctt ctttgagcag gtcggcgagg cggtcggcct
     2101 tctcgacacc ggcgaccggg acgaagcggt gctcgacgtc ggatgccgtc tgcttctcgg
     2161 actcggtctc gtgaaggatc gcttcgttgg tgtaggagcg ggccagacgg ccgacctcac
     2221 cgtcgagcgt cgccgagaag aacatggtct ggcgctcgcc ggggatgcgg tccacgagcc
     2281 ggtcgacctg cggctggaag cccatgtcga gcatgcggtc ggcctcgtcc aggacgagga
     2341 tccggatgct ggccagcgag atcaggcgcc gctgcgcgag gtcttccaaa cggccgggcg
     2401 tggcgaccag aatgtgcgcc ttcgcggcct tcatggcctg ttccttgatc gagacgccgc
     2461 cgtaggcaac cgcgaccttc aggccccggg cggtcgcgac atcgcgaagt tctacggcga
     2521 cctgcgaggc gagctcacgc gtcgggacca gcacgagcgc cgaaggccgc ttgtcggccg
     2581 ggtcgagccg ctcgacgatc ggaatggaga atgcgagcgt tttgcccgat ccggtctgcg
     2641 acttcaccag cacgtcgcgg ccctcgatcg cgtccgggat cgcaaggatc tgacaggcga
     2701 acggggcgtt gatgttgcgc ttggccagcg cgctcgagac ggacttggat acgccgagat
     2761 cggcgaaaga cttctgcatg tagatagctc aactcctggc ggcaagccgt gccgcttctc
     2821 gggaggttga tcgcctgccc gataccagaa ggagcgggag aagctcgacc tcacagggcg
     2881 gccctatgcc gcaccttgcg aacatcatac cccataaggc cggggggttg ccatggggga
     2941 gttcccgctc tataaccatg agtgggttta ctatttgtgc aacagcgctc ggatggcgga
     3001 gcagaagacg atcttctgag ccttggctat cccgatggat acccgtagcc gcagagccgg
     3061 gaccacgctc gcccaggcgt gccggacgcg gatcttgcca ccccggggcc cctccctcga
     3121 tcgagtgccc gcgatcgttc actgtgtgga ggagagcttt tgatgtccgg ccggaacggg
     3181 cgtcgccgct tgcccgaagg ctccctgatc gtcgatttcg tctgcgacga cgactcggtg
     3241 ggggtgatct gctacggcga gttcgacctc accaccgcat cgatcctcga cgatgcgatc
     3301 tcggacggcg cgggcagggc cgccggcagg accttggttc tggacctcag cggcctgcac
     3361 ttctgcagcg tggccatcct gcaaagactg cgccgggccg ccttgcttgc gcgagcctcg
     3421 gcgacgacgc tggaggtcgt cgaaggacgc gttctcgcga ggctcttgac ggtcatcgac
     3481 cccgaccgcc ggtgctacga gatcggggaa cgcgacggca gatcttgacg aaggtgctac
     3541 cgtcggcggt atggcgcggt attacgtcta catcgaccta agcccgtccg agggctacgt
     3601 cctgggggcc accctccggg agccgtggga cgacggcgcg ttccgggaga ccagcctggc
     3661 ggcggcgatc gccgggcagg aagcccggat ctacaccgac gaagagatgt gggacgaccc
     3721 ctcgcgacgc gcggcgctgg cgcaatggtt cgcggagcgg gacgactcgg tggacgacat
     3781 cggggtcgca cggccgagcc ttcggctgct caccgctccg ctcgacaccc gcacgattgc
     3841 cgaacgcagc cgcgcggcga tcgccgaggc gcggcgggcg gtgcgcgaag ccagcaagcg
     3901 ccgggaagag gcgcggcagg tgcgcgagcg acggaccccg cgccctacgc agccggaaag
     3961 gtccgcatga gcttcggcgc gcagcttcgg gacgcccgga ccgccgcggg gatgtcccag
     4021 gccgacctcg agcaggccac cggcatcccg aagtcgcgtc tgtcccgcta cgagaacgat
     4081 cacatcctgc cgtcggtgcc gagccttcgc cgtctcgccg aagcgctcgg cgtgaccgag
     4141 tcgcatctgg tcactcccaa gacggatccg tggacggcgc tgctgacgcg gctgcgcgca
     4201 tccggcctgc aactccggtc ggtcaaggaa gcggagtcga tcgccgactc gctcatcgcg
     4261 acgatcgcgg agcggcggcg ccgggaaatc tactgagcta aggcgagccg gcctcttcgg
     4321 ttagcaccac gaccccgcgt acgtcgccgg ccgcgcctcg cagcggc
//
LOCUS       DATLFO010000265        11690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_135119, whole genome shotgun sequence.
ACCESSION   DATLFO010000265 DATLFO010000000
VERSION     DATLFO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135119"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VM841_10885"
     CDS             <1..273
                     /locus_tag="VM841_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HVL90726.1"
                     /translation="TMCIVAPPSMLAVIADATGASSDIGEAVSVLPVEEAKGLEFDEV
                     VVAEPAALVKESPQGLRALYVALTRATRRVRLVHEQPLPEPLSQAP"
     gene            complement(334..975)
                     /locus_tag="VM841_10890"
     CDS             complement(334..975)
                     /locus_tag="VM841_10890"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVL90727.1"
                     /translation="MGLASFLDPDYLIATFGLIGIVLIVFAESGLFFGFFLPGDSLLF
                     TAGVLAAADKLNFALLLPLVFIAAVAGDQVGYLFGSRAGAALYRRPDSRFFKREHLEK
                     AHDFYVRRGASTIVLARFIPFVRTFAPIVAGAAKMPYRTFITFNVVGGFLWAVGVTTA
                     GFALGNAIPDIDKYLLPVIGVIVFLSILPLLIEARKHRRAKRQKAAEAATEQI"
     gene            complement(1056..1391)
                     /locus_tag="VM841_10895"
     CDS             complement(1056..1391)
                     /locus_tag="VM841_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90728.1"
                     /translation="MKRAVRSDEPAAGAAPARRLRPHPPATRSKRRPAAPQIHRPHDF
                     DALSPPPVRRRRRTRTKLLRLIRPSLLTIPGLPLIAVSALALVLVWVAVSLVVVTFTG
                     LLVTMILMG"
     gene            complement(1519..4344)
                     /locus_tag="VM841_10900"
     CDS             complement(1519..4344)
                     /locus_tag="VM841_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL90729.1"
                     /translation="MSVFVGRDHETATLERAVRGAVRQGRPHVVVLEGDPGSGKTRLL
                     AETLPAGIVANRVGIAAFEPERDLPLATGRSLLRSLAVAEPGLQPLDSFLDVRPGEGS
                     SEWAGIFEAAHRAASSLQPLLLSIDDVQWADGASIALLHYLVRGAEADRDPLILVVAG
                     RPSPAVTELSVALERLLGERHRRVVVGPLEHDAAIALVRAANPALDAAAAGQIAGRAG
                     GSPFWCRALARASDGKADIAAYVGSRLRGLTTDAAAAVTIPVLLGRPVPARDLAGILR
                     WPDDRVRAAIEPAAARGLLVEAGTAVRPAHDLIRSAIDASLDDADRLAAHRLIATWLD
                     ETAGDDPARLLAAARHRRAAGLSIDETLAAIVRSPRRGVLGRDGFEALAALAAEIDPD
                     PAALEIERGLAEIAGDLGRHRTAMRRWERLGERLPDASGRARAWLAAGEAAQRLEDAD
                     AAQACIARGRSERVDDPVLALEIDAAESATMRWLLHRPGDASRLAESVLTRVRAMVAE
                     ASGVDRLPEGARRAYVRALVQASVGAMQRSAVEELLTLADELAVAAAGVDVPAAVQAR
                     IRSGAALMLSGRLAEAERHLGEAWSHARRALLAEPSLDSGAWLAWTRSLMGRLADAEE
                     VAEECVALAERIGEHSRAASIAMLWRARIQVHRGDRDDAIATLRRLAGAESDGHHRIV
                     IQESIAIWLARLEGAAAAAEIRSRVGDGHADADAAGCARCRTEFLLAAAEALARIGDD
                     RAAAVLLREGSEAAPDGDLQRWNLLRAEASVAVAARAPDTAERVRDTVRAADRTGLGI
                     EAIWARIDLGRLLPDEAAVEALREAHHAAADAGARTEERLVEQLLRGRGVRTRGRSAS
                     APATLTPREREIAALIAEGAANLDIARVLFLSRKTVERHVSSILAKLGVKNRAELAAK
                     IASGEIARQERRIALISNSEPARPAVE"
     gene            4468..4782
                     /locus_tag="VM841_10905"
     CDS             4468..4782
                     /locus_tag="VM841_10905"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HVL90730.1"
                     /translation="MYVQVVTFRLGGIDETQYHEACKAETEPFATLPGLLAKIWLRDP
                     AGGIYGGLYLWRDREAHDAYVAGPIFDSIVKAPAFADVASKGFDVFDDLTKATQPGLS
                     LV"
     gene            complement(4880..5686)
                     /locus_tag="VM841_10910"
     CDS             complement(4880..5686)
                     /locus_tag="VM841_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVL90731.1"
                     /translation="MSERPFRFMTFNIRFATPADDAAGHGWTDRRDPVVSTIRACAPD
                     VLGVQEALRGQLDDLVAGLPDHQVIGKPREVGDKGEYVPLFVHRRFEIEQSGDFWLSS
                     TPEVEGSLGWDAPDPRHCTWARLRDAATGVRFAVFNTHLDRWGLIARVEAVRLIAARV
                     ALAPELAAVVMGDFNEPEDSEALDLLRASGLRDTFRVLHPAETNVQTVHQYTELPGTY
                     KIDYVMCDARWTVHAAEIVRRPAGGRLPSDHYPVVATLSLPEAGGAVAEP"
     gene            complement(5803..7242)
                     /locus_tag="VM841_10915"
     CDS             complement(5803..7242)
                     /locus_tag="VM841_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90732.1"
                     /translation="MNLPRRSVLALICAVATIAGNALAATGGTASSAVPRAICGPGSD
                     PETGMQGRVTAEDIDGGRAGRGFTCNTEIIGRHGSTLLTGGAGGYKVFRFVDAAGREC
                     GYYDSTLLLPMNVARAASDLPGVVVLDMADPSSPRRTANLVTPAMLSPHESMSINVER
                     GLLAAVAGNPLFKPGFVDIYDISQDCRKPVLMSSTPTGILGHEGAFSPDGNTFWVTST
                     NLGFITAIDVTNPRRPSIVWTTRGIGIHGFSISDDGTRFYGANAGRPGLTILDVSQVQ
                     ARAADPVVAEISHITWPSVSIPQHTIPVTIGGRPYLIEIDEFARGTVGDPNWNVGAAR
                     IIDISNERSPVIVSDIRLEVHDPGNGPAIKDDPGAQWGLGGYTGHYCGVPQRHEPGIV
                     ACSFILSGLRVFDIRDPRAPREIAYFNAPISAGPGAPGGSNGSVTPHAAYAMSAPAFV
                     PERAEIWYTDGTYGFFGLRVTNGVWPFTG"
     gene            7380..7643
                     /locus_tag="VM841_10920"
     CDS             7380..7643
                     /locus_tag="VM841_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90733.1"
                     /translation="MNPFNLMPSTRAGARSRRRFPVALWIVALLWIGAGFARSQEITL
                     RMYPAPGTSTASPTTQITMRGAGIDAIGKISVLGTARSTGSTP"
     gene            7640..8269
                     /locus_tag="VM841_10925"
     CDS             7640..8269
                     /locus_tag="VM841_10925"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HVL90734.1"
                     /translation="MTGEVIWRIGGTNSDFAVGPDTTFEFQHDARWLDSGILSLFDNG
                     AGPREVKEKSRGLHLKLDVESMTASLVREFPHPDGSLAGSQGNMQNLPNGTVVVGWGS
                     RRNVTRRERHGAVPRDDVADELLPRVAYAVARPADAATQFRGASIGIVGQGLRELERR
                     HQRHPMGAVGRTLGTIVAGRGSVQAPKLRDRALRPYVGDRLRRARPGLA"
     gene            8463..9116
                     /locus_tag="VM841_10930"
     CDS             8463..9116
                     /locus_tag="VM841_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVL90735.1"
                     /translation="MGSTATPSPKAEAHQALLDAAERLLVESGAAGVTTRRVAQEAGV
                     NHGLVHYYFGSIENLVAEVLERFTDKLLLRQRAMYAADAPFVDKWRAAMSYMDQDHES
                     GYHKIWLELHAMAWNRPELRERVARVTAEWRSVLAGALETAAREYGLRPAEAPVAAIT
                     ALIETATIGTMMERHIGVTTGHAELLAEIERRLERLERKKGSSDAGTASGRGRVRRP"
     gene            9073..11169
                     /locus_tag="VM841_10935"
     CDS             9073..11169
                     /locus_tag="VM841_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90736.1"
                     /translation="MRARLPDADGYVDRNGVKLFYEVFGSGDRTVLFMPTWAIVGSRY
                     WKMQIPSFARYARTITYDPRGHVHSDSPRGPESYSPEQYAADAIAVMDALGVERASVV
                     TLSIGARAGLVLAANHPDRVERIVFNCPAVPFGEAPDTRQSFPFEAELDTDEGWAKFN
                     RHYWMRDYEGFLRFFFEKNFTEPHSSKALEDALGWGAETTPEVLADTTLGGLVGFDEE
                     RARELASRIRCPVLVIQGSDDGITGPGRGIALAEATGGHLAMIEGGGHGPHARDPVRF
                     NLLVRDFFGLRPLARSHTRWNRRRKRALYVSSPIGLGHARRDAAIADELRKKHPDLEI
                     VWLAQSPVTDVLAQRGETIHPASRLLASEAAHIDDESGEHDLHVFQAFRRMDEILLAN
                     FMVFQDLMEDEHFDLVVGDEAWELDHFLHENPELKRAPFVWMTDFVGWLPMPEGGEHE
                     KYLTADYNAEMIEHIDRYPRLRDQSIFVGSPDDIVADTFGANLPSIRAWTEQHFRFAG
                     YTTGFDPAETADREALRARFGFAPDETVCLVTVGGSGVGAPLLRRVIDSYPIAKQKIP
                     GLKMLVVAGPRINPVSLPAHDGMEVRAFVPDLYRQMAACDAAIVQGGLTTCMELTAER
                     RPFLYFPLRNHFEQNFHVPHRLQRYNAGRRMDYAASPPDAIADALVEELARPVDYKPV
                     EYQGAIDAAAMISELL"
     gene            11228..>11690
                     /locus_tag="VM841_10940"
     CDS             11228..>11690
                     /locus_tag="VM841_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HVL90737.1"
                     /translation="MHRIARLLAAVLLVATLPAIAPAAPKAPVHVGSLTMEAATFWDG
                     GHVGSDPTYSLPFTGVPFPDRCASVDPCFVYTFDVTATSPTARLRVGLDTPARDDGFE
                     MTLISPANVAASRQNSNSYSMELFANAPETGTWTVKVVPVSADHASFRMRAK"
BASE COUNT         1865 a         4046 c         4053 g         1726 t
ORIGIN      
        1 accatgtgca tcgtcgctcc gccgtcgatg ctggccgtga tcgccgacgc caccggcgcg
       61 tcgtccgaca tcggcgaggc cgtctcggtt ctgccggtcg aagaggccaa agggctggag
      121 ttcgacgagg tcgttgtggc ggagccggcg gcgctcgtca aagaatcccc tcaagggctt
      181 cgtgcgctct acgtcgcact gacccgcgcg acccgccgcg tccgtttggt gcacgagcag
      241 ccgcttcccg agccgctgtc gcaggcgcct tgaacgtgcg gaacagcgca ccggccggcc
      301 acgggagacc gccgtgggat ggggcgccgg ctgctagatc tgctcggtgg cggcctcggc
      361 ggccttctgc cgcttggccc ggcggtgctt cctggcctcg atcagcagcg gaaggatcga
      421 cagaaacacg atcaccccga tgaccggcag caggtacttg tcgatgtcgg ggatggcgtt
      481 gccgagcgcg aagcctgccg tcgtcacgcc gaccgcccag aggaagcccc cgacgacgtt
      541 gaaggtgatg aaggtccggt agggcatctt ggccgcgccg gccacgatcg gcgcgaaggt
      601 cctcacgaac gggatgaacc gggccagcac gatcgtggac gcgcctcgcc gcacgtagaa
      661 atcgtgtgcc ttctccagat gctcgcgctt gaagaatcgg gaatcgggcc gccggtacag
      721 cgccgcgccc gctcgcgagc cgaacaggta gcccacctgg tcgccggcca ccgcggcgat
      781 gaacaccaac ggaagcagca gcgcgaagtt caatttgtcg gcggcggcca gcaccccggc
      841 ggtgaacagc aagctatcgc ccggcaggaa gaagccgaag aaaagacccg actcggcgaa
      901 gacgatcagg acgatgccga tgagcccgaa ggtcgcgatg agatagtcgg ggtccagaaa
      961 gctcgccaat cccatgatgc cggaacgata ccgagttccg ggccgccgtg cttcacccga
     1021 agggggatgg ttcgctcgcg tgagcggtgg gcacgttagc ccatcaggat catggtgacc
     1081 agaaggcccg tgaacgtgac gaccacgagc gaaacggcca cccagacgag gaccagcgcg
     1141 agcgcggaga cggcgatgag cggcaatccg ggaatcgtca gaaggctcgg ccggatcaaa
     1201 cgaagcagtt tcgtgcgcgt ccggcgccgc cggcgcacgg gcggcggcga cagcgcgtcg
     1261 aagtcgtgcg ggcgatgaat ctgcggcgcg gccggccgcc tcttcgaacg cgtcgccggc
     1321 ggatgaggac gaagacgtcg tgccggtgcc gcgcctgcgg ccggctcgtc ggatcgaacg
     1381 gcgcgtttca cgattgtctc ctgtcgtact ctccggcgcg ccgcggggtc aaggggccgc
     1441 aaagtttggg gacggaggaa ttccctagtc cgttcgggcg attcgctcgc cggcgtgtgc
     1501 tcctggacgg agcggaagtc attcgacggc gggccgagca ggctcactgt tcgagatcag
     1561 cgcgatccgc cgctcctgcc gcgcgatctc cccgctcgcg atcttcgcgg caagctcggc
     1621 gcgattcttg acgccgagct tggccaggat gctcgagacg tgccgctcga cggtttttcg
     1681 ggacaggaac agcacccggg cgatgtcgag gtttgcagcg ccctcggcga tcagagcggc
     1741 gatctcgcgc tcgcggggcg tgagcgtggc cggggccgag gcacttcgtc cgcgcgtgcg
     1801 cacgccgcgg ccgcgaagaa gctgctccac gaggcgctct tccgtcctcg cgccggcgtc
     1861 tgccgccgcg tggtgtgctt cccgcaaggc ttcgacggcg gcttcatccg gaaggagccg
     1921 gcccaggtcg atccgcgccc agatcgcctc gatgccgagc ccggttcggt ccgcggcccg
     1981 gacggtatcg cgcacccgtt cggccgtgtc gggtgcgcga gcggcgacgg ccaccgacgc
     2041 ctcggccctc agcaggttcc agcgctgcag gtccccgtcc ggcgctgcct cgcttccctc
     2101 gcgcagcagc accgccgcgg cgcgatcgtc accgatgcgg gcgagcgctt cggcggcagc
     2161 gagcaggaac tcggtccggc agcgcgcgca gcctgcggca tcggcatcgg cgtgtccgtc
     2221 gccgacgcgc gagcggatct cggccgccgc cgcggctcct tcgagccgcg cgagccagat
     2281 ggcgatcgac tcctggatca cgatccggtg gtgcccgtcg gactcggcgc cggcgaggcg
     2341 acgcagcgtc gcgatcgcgt cgtcgcgatc gccgcgatgc acttggatgc gcgcgcgcca
     2401 caacatcgcg atgctcgcgg ctcgcgaatg ctcgccgatg cgttcagcga gcgcgacgca
     2461 ttcctcggcc acctcctcgg cgtcggccaa acggcccatc aaggagcgcg tccatgcgag
     2521 ccaggcgccc gagtcgagcg acggctcggc cagcagcgcg cgtcgcgcgt ggctccacgc
     2581 ttcgcccaga tgccgctcgg cctcggcgag ccggcccgag agcatgagcg cggcgccgct
     2641 gcgaatgcgg gcctggacgg cggccggtac atcgacgccg gcggccgcga cggcgagctc
     2701 gtcggcgagc gtgagcagct cctcgaccgc gcttcgctgc attgccccga cgctcgcttg
     2761 gacgagcgcg cgcacgtacg cgcggcgcgc accctcgggc agccggtcca cgcccgacgc
     2821 ctcggccacc atcgcgcgaa cgcgggtcag gacggattcg gccagccggg aggcgtcacc
     2881 gggccggtgc agcagccagc gcatcgtcgc cgactcggcg gcgtcgatct cgagcgcgag
     2941 cacgggatcg tccacgcgtt cggagcggcc gcgagcgatg caggcctgcg ccgcatccgc
     3001 gtcctcgagc cgttgggccg cctccccggc cgcgagccac gcacgcgcgc ggccgctcgc
     3061 gtcgggcaac cgctcgccga ggcgctccca acgccgcatc gccgtgcggt gccgcccgag
     3121 gtcgccggcg atctcggcaa gaccgcgttc gatctcgagc gccgcaggat ccggatcgat
     3181 ctcggccgcg agggccgcca atgcctcgaa gccgtcgcgg ccgagcacgc cccggcgcgg
     3241 cgaacgcacg atcgcggcca gcgtttcgtc aatcgaaagc ccggccgcac gccggtgccg
     3301 ggccgcagcg agcagccgcg ccggatcgtc accggcggtc tcgtcgagcc aggtcgcgat
     3361 cagccggtgc gcggcgagac ggtcggcgtc gtcgagcgag gcgtcgatcg cggaacggat
     3421 cagatcgtgc gcgggccgca cggccgtgcc cgcttcgacg agcaggccac gcgcggcggc
     3481 aggctcgatc gcggcgcgca cgcgatcgtc gggccatcgc aggatgccgg ccaggtcccg
     3541 ggccggcacc ggccggccga gcagcaccgg gatcgtgacc gccgccgccg cgtccgtcgt
     3601 caggccgcgc agccgcgatc cgacgtaggc ggcgatgtcg gctttgccgt cgctcgctcg
     3661 cgcgagcgcc cggcaccaga acggcgagcc ccccgcgcgg ccggcgatct ggccggccgc
     3721 ggccgcgtcg agcgccgggt tcgcggcgcg tacgagcgcg atcgccgcgt catgttcgag
     3781 cgggccgacg acgacgcggc gatgccgctc gccgagcagc cgttcgaggg cgaccgacaa
     3841 ctccgtgacc gccggagagg gccgcccggc gacgacgagg atcagcgggt cgcggtcggc
     3901 ctcggcaccg cgcaccagat agtgcagcag cgcgatcgac gcgccgtcgg cccactgcac
     3961 gtcgtcgatc gatagcagca gcggctgcag ggaggaggcc gcgcgatgcg cggcctcgaa
     4021 gatcccggcc cattccgaag acccttcgcc gggacgaaca tcaaggaatg aatccagcgg
     4081 ctgcaggccg ggctcggcaa cggcaagcga tcgcagcagc gaccggccgg tcgcgagcgg
     4141 aaggtcgcgc tcgggctcga acgctgcgat gccgacccgg ttcgcgacga ttccggccgg
     4201 gagcgtctcg gcgagcagac gcgtcttgcc cgaaccgggg tcgccttcga gcaccacgac
     4261 gtggggacgc ccctgccgca ccgcgcctcg caccgcgcgc tcaagcgtcg cggtctcgtg
     4321 atcccgtccg acgaagacgc tcatggtcga agggtagccc ccggaggccg cggacatcca
     4381 cgccgcatca tttgagggag atccctcatg atcgccgccg ctcgccttcg tatcgtcggg
     4441 atcggtcacc ggacgaagga ggagccaatg tacgtccaag tcgtcacgtt ccggctcggc
     4501 ggcatcgacg agacccaata ccacgaggcc tgcaaagccg agaccgagcc gttcgcgacc
     4561 ctgccgggat tgcttgccaa gatctggctc cgcgatccgg ctggcggaat ctacggcggc
     4621 ctgtacctgt ggcgcgaccg agaggcgcac gacgcctatg tcgccgggcc gatcttcgat
     4681 tcgatcgtca aggccccggc attcgccgat gtggcgtcca aaggcttcga cgtcttcgac
     4741 gatctgacga aggcaacaca gccgggcctg tcgctcgtgt agcgcacgcc tcacggaccg
     4801 ggccgaaccg ggccgggccg ggcggactcc gtgttgtccg gaccgacagg agaccggacc
     4861 gcgcgcggca atgtggtcgt cacggttcgg cgacggcgcc accggcctcg ggcaggctca
     4921 gcgtggcgac cacgggatag tgatccgagg gcagccgtcc gccggcgggg cggcgaacga
     4981 tctcggcagc gtgcacggtc catcgggcgt cgcacatcac gtagtcgatc ttgtacgttc
     5041 ccggcagctc ggtgtactgg tggaccgtct gcacgttcgt ctcggccgga tgcagcacgc
     5101 ggaacgtgtc gcgaagaccc gaggcgcgaa gcaggtccag tgcctcgctg tcctcgggct
     5161 cattgaagtc acccatgacg acggcggcaa gctcgggggc gagtgcgacc cgcgccgcga
     5221 tcagccggac cgcttcgacg cgcgcgatca ggccccagcg atccagatgt gtgttgaaca
     5281 cggcgaagcg cacgccggtc gccgcgtcgc gcagccgcgc ccacgtgcaa tgccgcggat
     5341 cgggagcgtc ccaacccaag gatccttcga cctcgggcgt ggacgagagc cagaaatcac
     5401 ccgactgctc gatctcgaag cggcggtgca cgaacagcgg aacgtactcg cccttgtcgc
     5461 ccacctctcg cggctttccg atcacctgat ggtcgggcag ccccgccacg aggtcgtcga
     5521 gctggccgcg aagtgcttcc tgcacgccga gcacgtccgg cgcgcacgcc cggatcgtgg
     5581 acacgaccgg gtcgcgccgg tccgtccagc cgtgaccggc cgcatcgtcc gccggggtcg
     5641 cgaaccggat gttgaacgtc atgaagcgaa acggtcgctc gctcatcgac cctcgttctt
     5701 cccagaagag gcgggtgtta cccgggcgtg gccgatgcgc cggcttcgga acggccggcg
     5761 gcgcgaccga gcgcttcgtg cagacgctcg cggatcgggt ccttatccgg tgaacggcca
     5821 cacgccgttg gtgacgcgca ggccgaagaa cccgtaggtc ccgtccgtgt accagatctc
     5881 tgcgcgctcg ggcacgaacg ccggggccga catcgcgtac gcggcgtgcg gcgtgaccga
     5941 gccgttgctg cctcctggcg cgccagggcc ggcggagatc ggcgcgttga agtaggcgat
     6001 ctcgcgcggt gcgcgcgggt cacggatgtc gaagacgcgc aggccggaca ggatgaagct
     6061 gcacgcgacg atgccgggct cgtgacgctg cggcacgccg cagtagtggc cggtgtagcc
     6121 gccgagcccc cactgcgcgc cgggatcgtc cttgatcgcc gggccgttcc ccggatcgtg
     6181 cacctcgagc cggatgtcgg acacgatcac ggggctgcgt tcgttcgaga tgtcgatgat
     6241 gcgtgcggca ccgacgttcc agttcgggtc gccgacggtt ccgcgcgcga actcgtcgat
     6301 ctcgatcagg tagggccggc cgccgatggt gaccgggatg gtgtgctgcg gaatgctgac
     6361 gctgggccag gtgatgtggc tgatttcggc cacgaccgga tcggcggcgc gcgcctggac
     6421 ctggctcacg tccaaaatcg tcaggccggg acggccggca ttggcgccgt agaagcgcgt
     6481 tccgtcgtcg ctgatgctga agccgtgaat cccgatgccg cgtgtggtcc acacgatgct
     6541 cggcctgcgc gggttcgtca cgtcgatcgc ggtgatgaac cccaggttcg tcgaggtgac
     6601 ccagaaggta ttgccatcgg gtgagaacgc gccttcgtgt ccgaggatgc ccgtgggggt
     6661 gctcgacatc aggacgggct tgcggcagtc ctggctgatg tcgtagatgt cgacgaagcc
     6721 gggcttgaac agcgggttgc cggcgacggc cgccagcagg ccgcgctcga cgttgatgct
     6781 catcgactcg tgcgggctca gcatcgcggg cgtgaccaga ttcgcggtcc gcctcggcga
     6841 cgacggatcg gccatgtcga gcacgacgac gccgggcagg tcgctggcgg cgcgagcgac
     6901 gttcatcggc agaagcagcg tcgagtcgta gtagccgcat tcgcggcccg cggcgtcgac
     6961 gaagcggaag accttgtagc cgcccgcgcc tccggtcagc agcgtgctgc cgtgacggcc
     7021 gatgatctcg gtgttgcagg tgaagccgcg cccggcgcgt ccgccgtcga tgtcctccgc
     7081 ggtaacgcgt ccctgcatgc ccgtctccgg gtccgagccg ggcccgcaga tcgcgcgcgg
     7141 cacggcggag gacgccgtgc cgcccgtggc ggcgagggcg ttgccggcga tggtagctac
     7201 ggcgcatatg agcgccagaa ccgacctgcg tggaagattc atcaagttcc tcttttccgg
     7261 gggcggcctt gccgctgcga ccgatacccc tcaatacgac gtccaaggga cgcatccctg
     7321 ctcccaaagc aacacaagcc acgtatccgt gagccttgcg acgataccgg gcacacggta
     7381 tgaatccctt caacctcatg ccttcgactc gcgccggcgc tcgatcccgc agaaggttcc
     7441 ctgtcgccct gtggatcgtc gcgctgctct ggatcggcgc cggattcgcg cgcagccaag
     7501 agatcacgct gcggatgtat ccggcaccgg gaacttcgac ggcctccccc acgacgcaga
     7561 tcacgatgcg cggcgcgggc atcgacgcga tcggcaagat cagcgttctg ggcacagcgc
     7621 gatctaccgg atcgacgccg tgacgggcga ggtgatctgg cgcatcggcg gaaccaacag
     7681 cgacttcgcg gtgggcccgg acacgacctt cgagttccag cacgacgcgc gctggctgga
     7741 cagcggcatc ctgtcgctgt tcgacaacgg ggccggtccc cgcgaggtca aggaaaaatc
     7801 gcgcgggctg catctaaagc tcgatgtcga atcgatgacg gcgtcgctgg tgcgcgagtt
     7861 cccgcatccg gacggatcct tggccgggag ccaaggcaac atgcagaacc ttccgaacgg
     7921 caccgtcgtg gtcggctggg gaagccgccg caacgtcacg cgacgcgaac ggcacggtgc
     7981 tgttccacgc gacgatgtcg ccgacgaact cctaccgcgc gtggcgtacg ccgtggcgcg
     8041 gccggccgac gcagccaccc agtttcgtgg tgcatcgatc gggatcgtcg gtcaaggcct
     8101 acgtgagctg gaacggcgac accagcgtca cccgatggga gctgttggcc ggacgctcgg
     8161 cacgatcgtt gcgggtcgtg gctcagtcca agcgccgaag cttcgagacc gcgctctacg
     8221 cccgtacgtc ggcgaccgtc ttcgccgtgc gcggcctgga ctcgcgtagc cgcgtgctca
     8281 ccacgacggc cgctcgctct gtctgatctc ccggtcacca cggctgcgca gccgctcgcg
     8341 ccgccgccgc cggcgatcag ggtcaccgcg ccggccgcgt ccatcgcatg tcgcttgacc
     8401 gccggggctc ccggtgttag tcatatgcct aagtcaatcg actaacccgt gagggaggac
     8461 aggtggggag caccgcgacg cccagtccca aggccgaggc gcatcaggcc ttgctggacg
     8521 cggccgaacg cctcctggtc gagtcgggag cagccggggt gaccacccgc cgggtggcac
     8581 aagaggccgg cgtcaaccac gggctggtgc actactactt cggctcgatc gagaacctgg
     8641 tcgcggaagt cctcgagcga ttcaccgaca agctgctgct gcggcagcgc gcgatgtacg
     8701 cggccgacgc gccgttcgtc gacaaatggc gagccgcgat gtcctacatg gaccaggacc
     8761 acgaatccgg ctaccacaag atctggctcg agcttcacgc catggcgtgg aaccggcccg
     8821 agcttcgcga gcgggtcgcc cgtgtcaccg ccgagtggcg ttcggttctg gccggcgcgc
     8881 tcgaaacggc agcccgcgaa tacgggctcc gacccgccga ggcgccggtc gccgcgatca
     8941 ccgctctgat cgaaaccgcg acgatcggga cgatgatgga gcggcacatc ggcgtgacga
     9001 ccggacacgc cgagctgctg gccgagatcg agcggcggct cgagcggctg gaacgaaaga
     9061 aaggatcgag cgatgcgggc acggcttccg gacgcggacg ggtacgtcga ccgtaacggc
     9121 gtcaaactgt tttacgaggt tttcggcagc ggggaccgca cggtgctgtt catgccgacc
     9181 tgggcgatcg tgggatcgcg ctactggaag atgcagatcc cctccttcgc gcgctacgcg
     9241 cgaacgatca cctacgaccc gcgtggacac gtccactcgg acagtccgcg cggcccggaa
     9301 agctactcgc ccgagcagta cgccgccgat gcgatcgcgg tgatggatgc actgggcgtc
     9361 gagcgcgcgt ccgtcgtcac gctgtcgatc ggtgcacgcg cgggcctggt tctggcggcg
     9421 aaccatcccg accgtgtgga gcgcatcgtg ttcaactgtc cggccgtgcc gttcggcgaa
     9481 gcgcccgata cccgtcagag ctttcccttc gaggccgagc tggacaccga cgaaggatgg
     9541 gcgaagttca accgccacta ttggatgcgc gactacgaag gcttcttgcg gttcttcttc
     9601 gaaaagaact tcaccgagcc gcattccagc aaggcgctgg aagacgcgct gggatggggc
     9661 gccgagacca cacccgaggt tctggccgac accacgctcg gcgggctcgt cggcttcgac
     9721 gaagaacgcg cccgcgagct cgcgtcgcgg atccggtgcc cggtgctggt gatccagggc
     9781 agtgacgacg gcatcaccgg cccggggcgc ggcatcgcgc tggccgaagc gaccggcggg
     9841 catctggcga tgatcgaagg cggcggccat ggcccgcacg cgcgcgaccc ggttcggttc
     9901 aacctgctgg tgcgggactt cttcggactg cgaccgctgg cgcgttcgca tacgcggtgg
     9961 aatcgacggc gcaagcgcgc actgtacgtg tcatcgccga tcggcctggg ccatgcgcgc
    10021 cgcgacgccg cgatcgccga cgagcttcgg aagaagcatc cggatctcga gatcgtgtgg
    10081 ctcgcccagt caccggtgac cgacgtgctg gcccaacgcg gcgaaaccat ccatccggcg
    10141 tcacggctgc tggccagcga agccgcccac atcgatgacg agtccggcga gcacgacctg
    10201 cacgtgttcc aagcattccg gcgcatggac gagatcctgc tcgccaactt catggtcttc
    10261 caggacctga tggaagacga gcatttcgat ctcgtcgtgg gcgacgaggc atgggagctt
    10321 gaccatttcc tgcacgaaaa ccccgagctg aaacgcgccc cgttcgtgtg gatgaccgac
    10381 ttcgtcggct ggctgccgat gcccgaaggc ggcgagcacg aaaagtatct gaccgccgac
    10441 tacaacgccg agatgatcga gcacatcgac cgctatcccc gcctgcgcga ccagtcgatc
    10501 ttcgtcggca gccccgacga catcgttgcg gacaccttcg gcgcgaacct gccgtcgatc
    10561 cgcgcatgga ccgagcagca tttccgcttc gccggttaca ccaccggatt cgatccggcc
    10621 gaaaccgccg accgcgaggc gctgcgcgcg cggttcggat tcgcgccgga cgagaccgtg
    10681 tgcctggtga ccgtcggcgg ctcgggtgtc ggcgcgccgc tgcttcgccg cgtgatcgac
    10741 tcgtatccga tcgcgaagca gaaaatcccc ggcttgaaga tgctcgtcgt cgcggggccg
    10801 cgcatcaacc ctgtatcgct gccggcgcac gacgggatgg aggtgcgcgc gttcgtaccc
    10861 gacctgtacc ggcagatggc cgcgtgcgac gccgcgatcg tgcagggcgg tctgacgacc
    10921 tgcatggagc tgaccgcgga acgccggccg ttcctgtact tcccgctgcg aaaccacttc
    10981 gagcagaact tccacgtgcc gcaccgcctc cagcgctaca acgccggccg ccgcatggac
    11041 tacgcggcct cgccacccga cgcgatcgcc gacgccctgg tcgaggagct ggccagaccg
    11101 gttgactaca agcccgtgga gtaccaaggc gcgatcgacg ccgccgcgat gatctccgag
    11161 ctgctgtagg gcttcggggg aggagtcacg gcccccgggg cgaagtatcc ctctcaagga
    11221 gggaatcatg catcgaatcg ctcgtctgct ggccgcggtt ctgctggtcg ccacgcttcc
    11281 tgcgatcgct ccggccgcgc cgaaggcacc cgtgcacgtc ggatcgctca ccatggaagc
    11341 cgcgacgttc tgggacggcg gccacgtcgg gtccgatccg acctacagcc tgccgttcac
    11401 cggagtgccg ttcccagacc gctgcgcctc ggtggatccg tgcttcgtct acaccttcga
    11461 cgtgaccgcg acctccccga cggcacggct gcgcgtcggg ctggacacac cggcccgtga
    11521 cgacggcttc gagatgacgc tgatctcgcc cgccaacgtc gccgcctcgc ggcagaactc
    11581 gaactcctac agcatggagc tgttcgcgaa cgcgccggag accggaacct ggacggtgaa
    11641 ggtcgtgccg gtcagcgccg accacgcctc gttccgcatg cgcgctaagc
//
LOCUS       DATLFO010000266         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_115432, whole genome shotgun sequence.
ACCESSION   DATLFO010000266 DATLFO010000000
VERSION     DATLFO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115432"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VM841_10945"
     CDS             complement(<1..414)
                     /locus_tag="VM841_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90738.1"
                     /translation="MSAGNATTTTNGELRMVRSALLVVSAATPPTAAIAWLLRGPEGA
                     ISVLLAVAIVLANAFVSAAVLAFAVKRSPLMGPSLQMPSYAFRMAGIVFVLAKLRNAS
                     FVDVPTFAICFAVAVVATLAVEARVYKRTPWVALTF"
     gene            complement(411..746)
                     /locus_tag="VM841_10950"
     CDS             complement(411..746)
                     /locus_tag="VM841_10950"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HVL90739.1"
                     /translation="MPRASDQDERRRLYAGASDGMSKAIDLVTATAVWGAIGWLIDRW
                     LGTWPIVFAIGTVIGNLTGIYILYRRSIEEGTRASSGTAAGVTRSMPRHTRPARGDAG
                     ARVDGGDDE"
     gene            complement(1017..2159)
                     /locus_tag="VM841_10955"
     CDS             complement(1017..2159)
                     /locus_tag="VM841_10955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HVL90740.1"
                     /translation="MTAHLIVMAAAAAVTFVVTPLYRRVAFRLGAVDVPGAEDRKVHR
                     DPTPTSGGVAIFAGVAAGFFAAKLMGAFDSMFSTSTEPFGILAASLLLVVIGIVDDVR
                     GMRAPAKLAAQAIAAGIIVLSGVQFLFFWLPGVGLVSLSIDLAALLTVVWTVAVINGV
                     NLIDGLDGLAAGVTAIAAGALFVYAFQAADGQPTVATLVPMLVFGACLGFLPHNFNPA
                     KIFMGDTGSMLLGLLLASSTVSGVGRTIEPRFSDVAGLIVPVLLPLFVLAIPLADIGF
                     AITRRAKAGKPVFHADKSHIHHWLLDMAHSHRRAVLVMWSWSAMLASAAMVLSIGYGT
                     GPRIVAGAIVATLIASIVFVPRALRRDRSEPAAARASDRAAPRRPR"
     gene            complement(2156..2293)
                     /locus_tag="VM841_10960"
     CDS             complement(2156..2293)
                     /locus_tag="VM841_10960"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HVL90741.1"
                     /translation="MGQRACGEPVALKILDIWIRTPFDGGRHAERIARIAEIEAGERG
                     A"
BASE COUNT          393 a          803 c          779 g          320 t
ORIGIN      
        1 gaaggtcagg gcaacccacg gcgtgcgctt gtagacccgc gcctcgaccg cgagcgtcgc
       61 gacgacggcc accgcgaagc agatggcgaa cgtcggcacg tccacgaacg aggcgttgcg
      121 cagctttgcg agcacgaaca cgatgccggc catgcggaac gcgtaggacg gcatctgcag
      181 cgacggcccc atcaggggcg agcgcttgac ggcaaaggcc aggaccgcgg ccgacacgaa
      241 tgcgttggcc agcacgatcg cgacggccag cagaacggag atcgcgccct cgggcccgcg
      301 gagaagccag gcgatcgcgg cggtgggcgg cgtcgcggcg gagacgacga gcagcgcgct
      361 gcgcaccatc cgcagctcgc cgttcgtcgt cgtcgtcgcg ttgcctgcgc tcattcgtcg
      421 tccccgccat cgacgcgagc tcccgcgtcg ccgcgcgccg gccgggtatg tctcggcatc
      481 gaccgggtca ctccggccgc cgtcccgctc gacgcgcgcg ttccttcctc gatcgaccgt
      541 cgatagagga tgtagatccc ggtgaggttc ccgatcaccg tcccgatcgc gaacacgatc
      601 ggccaggtgc ccagccagcg atcgatcagc catccgatcg ctccccaaac cgcggtggcc
      661 gtgaccaggt cgatcgcttt ggacattccg tcgctggcgc cggcgtagag cctgcgccgt
      721 tcgtcctggt ccgaggcccg tggcattaaa cccgttgccc cccgcgcatg tcaaaacgca
      781 gcaggaatcc gttttcgcag gtcaggggca tttttctggg ccggtcgctc cgagattgtg
      841 aatcgcttca caagaagtat gcccaggagg gtggacgggg tcaaaccggg cccgcgtctc
      901 cccgactctg ctgatgccgt cagtgccgcc gcatcggtcg tctgccgggc agccgcgctc
      961 gacgttccgg tcgcaccaca ctccggtcga caggtcgtgc cgccgcgttc ggcatctcat
     1021 ctcggtcttc ggggagccgc gcgatcggag gcgcgagccg cggcgggctc gctgcggtct
     1081 cggcgaagcg cgcggggaac gaacacgatg gatgcgatca gggtcgcgac gatcgcaccc
     1141 gcgacgatcc gcggcccggt gccgtagccg attgatagca ccatcgcggc gctcgcgagc
     1201 atcgcgctcc aggaccacat caccagcacg gcgcgacggt ggctgtgggc catatcgagc
     1261 agccagtggt ggatgtggct cttatcggcg tgaaagacgg gcttgccggc cttggcccgg
     1321 cgcgtgatcg cgaagccgat gtccgccagc gggatcgcca gcacgaacag cgggagcagc
     1381 accggaacga tcaagccggc cacatccgag aacctcggct cgatggtccg cccgacgccg
     1441 ctgacggtgc tggaggccag cagcagcccg agcagcatcg agccggtatc gcccatgaag
     1501 atcttggcgg ggttgaagtt atgcggcaga aagccgaggc aggcgccgaa caccagcatc
     1561 gggaccagcg tcgcgaccgt gggctgtccg tcggcggcct ggaacgcgta gacgaacagc
     1621 gcgccggccg cgatcgcagt gacgcccgcg gccagcccgt cgagtccgtc gatcaggttc
     1681 acgccgttga tcacggccac cgtccacacg accgtcagca acgcggccag gtcgatcgac
     1741 agcgacacca gtcccacgcc gggaagccag aagaacagga actgcacgcc cgacagcacg
     1801 atgattccgg cggcgatcgc ctgcgcggcc agcttggcgg gcgcgcgcat gccgcgcacg
     1861 tcgtcgacga ttccgatcac caccagcagc agcgacgccg ccaggatgcc gaacggctcc
     1921 gtggaggttg agaacatcga gtcgaacgcg cccatcagct tcgcggcgaa gaatccggcc
     1981 gcgacgccgg cgaagatcgc cacgcccccc gaggtggggg tcgggtcgcg atgcactttt
     2041 cgatcctcgg caccggggac gtccacggca ccgagacgga acgcgacccg tcggtacagc
     2101 ggcgtcacga cgaaggtgac ggccgcggcc gcggccatca cgatcaggtg cgcggtcacg
     2161 cgccccgctc tcccgcttcg atctcggcaa tgcgcgcgat gcgctccgcg tgccggccgc
     2221 cgtcgaaggg cgttcggatc cagatgtcga ggatcttgag cgccacgggc tcgccacatg
     2281 cgcgctgtcc cacgc
//
LOCUS       DATLFO010000267        10127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_144272, whole genome shotgun sequence.
ACCESSION   DATLFO010000267 DATLFO010000000
VERSION     DATLFO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144272"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VM841_10965"
     CDS             <1..770
                     /locus_tag="VM841_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HVL90742.1"
                     /translation="PGAAPPWPSDDGPPPLPDGATESEPAALPPVPWHWLESFAVFML
                     TFVTAGLLAVVVAAAFSGDLEKAMAVVVFEVALAGWALLWPKIRFGLGPRALGLRRDQ
                     MGGDIRYGLGIGVVGWVVSTLVVGNVVVRLVERITDGPVTEPDQLNYSDPSGLVLTIT
                     AIGVIVIAPIAEEIFFRGFVFRGFRRDVGFVIAAVASSILFMFAHLPFWIIFPSIFTL
                     GLFLSRALEKRASLIPCIVAHALFNSAGFAAYMITLN"
     gene            complement(789..1481)
                     /locus_tag="VM841_10970"
     CDS             complement(789..1481)
                     /locus_tag="VM841_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90743.1"
                     /translation="MATFAFVHGAFMRGAHFAVVAERLRGAGHRTVAPDLPATDPAAG
                     AAEYARLVAAAAGDTGDLVVVAHSMGGLTAPVVATLRPVRRIVYLAALVPEPGRSFDD
                     VSWHDPRIAAPYVPQTHSLASEDGSASVGPARAAEVYFHDASPARRQWAYSLMRPQQW
                     KILREPCPLDAMPSVDATVVACRQDRLVSPSWLSHVASERLGTEAITIDGGHCPMVSR
                     PGELVRLLIALA"
     gene            1574..1996
                     /locus_tag="VM841_10975"
     CDS             1574..1996
                     /locus_tag="VM841_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HVL90744.1"
                     /translation="MALFPSEDWMKLYVEKINSSKDYEEAASTWEGDIAYVFEAEPDK
                     GVPNDVWSWADLWHGKCRDSKWDVTPEEGEKAKFIIRAPYSRWKEVIRKELDPVKGMM
                     QGKLKLKGDLPTIVRYVKASNELVNLASQVPTEFVDEQ"
     gene            1996..2409
                     /locus_tag="VM841_10980"
     CDS             1996..2409
                     /locus_tag="VM841_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90745.1"
                     /translation="MEYVTTDPEIDGLGAMLADLVRANIAAHPERAKLLENVSGTINV
                     KAIDAGVAVGLEFAANRFNVFAKPFRRAGLEIITDSGTLMELTAVPLWKGQPDVRTPE
                     GRTVLRKMVRRDLRIKGAVAHPMLLNRLQRLLSAV"
     gene            2406..3077
                     /gene="npdG"
                     /locus_tag="VM841_10985"
     CDS             2406..3077
                     /gene="npdG"
                     /locus_tag="VM841_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HVL90746.1"
                     /translation="MSGARVVAFVGGTGPAGFGLAARFAKGGVPVVIGSRSVERAEEA
                     RTQVLERVPDADCRAMLNEDAIAAGDIVFLTIPFQATRHTVETLAGHLAGSIVVSMAN
                     PVWVHAGKVRADTPPAGSLAEEVQELVPSARVVGAFHEVNVRKFPLIDKPINSDTIVT
                     SNDPEAKRMVMDLVRHVDGMRAVDGGELLNTRYVEGFVCVLVTANFIYKASSALRLTG
                     LPEEQ"
     gene            3074..3682
                     /locus_tag="VM841_10990"
     CDS             3074..3682
                     /locus_tag="VM841_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90747.1"
                     /translation="MRRGLVVALVAATACTPAVSPESGPDPVPTISGSPAPATGLAAL
                     LFDEAPPGYVVVPEETRAVTLDEAVEGSDDPDEARARLSTHGFVSGFTRTWTKDDDAG
                     NVIYAFVYEFATEEGAQADMNAGLGEARRDGSETFDVPGIEGAIGVTKAAAAGAEGDD
                     STFHAVFFVRGKRLYLVAIGGPTEPSHEQAVELAKRMDVRAR"
     gene            complement(3679..4425)
                     /locus_tag="VM841_10995"
     CDS             complement(3679..4425)
                     /locus_tag="VM841_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90748.1"
                     /translation="MAEPLRKPDAPAFYALRTGGWRDYVTLLHPPYTAWHLSYVAIGW
                     SVAETVHVDRLVALLIAFFLAVGIGAHALDELNGRPLRTQIPSRALWVLAAVSIGLAC
                     GIGIAGAARTRWTLLALVAFGGFVVAAYNLEWFGGRFHSNRWFALAWGAFPALTASFA
                     SDLRPTLPAAGAAAACFALSLAQRTLSTPVRALRRRTRSVTGHVERSDGSVELIDADW
                     LRRPPEAALRALSFALPLLALALVVSRLVA"
     gene            complement(4406..5101)
                     /locus_tag="VM841_11000"
     CDS             complement(4406..5101)
                     /locus_tag="VM841_11000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90749.1"
                     /translation="MASERSAGARALFAGIAPAYDRPAAVLSFGEYGRWRRALVRALP
                     LADGDAVLDVACGTGLISRDLEQIAGARVTGLDQSLPMLTRGAGARVAGQAERLPFAG
                     ATFDGVVFSYLLRYVDDPAATIAEMARVLRPGGVMGSVEFGVPRARLPRLGWRIYSRG
                     VFPWAARIVSPGWGWTGQFLPGSIMELDASLPPEKQAVLWQDAGLTDVRIKRMTFGTG
                     VLMMGRKDGRAAP"
     gene            5202..6146
                     /gene="meaB"
                     /locus_tag="VM841_11005"
     CDS             5202..6146
                     /gene="meaB"
                     /locus_tag="VM841_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014176347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HVL90750.1"
                     /translation="MDVSALVEGLRSGDRRTVARMISLVEDASIETLGEVMRQIGGMT
                     SHAYTIGVTGSPGVGKSTLTGALVSELRKRDRTVSVLAIDPTSHITGGALLGDRLRMQ
                     EHATDPGVFIRSMATRGHLGGLAWAAPHALRILDAAGVDTILVETVGVGQDEVEISAQ
                     ADTTIVALAPGMGDSVQANKAGILEIADVYVVNKADREGAKQVVRDIQQMLELGAHGE
                     WLPPVLMTTATDGTGVDEVLNAVDRHREYMDSSGEARRRRLARAAGEIREIVIGRMRA
                     AIGTVKDSDLLAALAEQVVEHELDPYAAADKLLAEVDA"
     gene            6143..7801
                     /locus_tag="VM841_11010"
     CDS             6143..7801
                     /locus_tag="VM841_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVL90751.1"
                     /translation="MTEGAYEQWRAAYEAGLKREADFDTLSGEVLEPLYTPADVEDID
                     YETDIGLPGQFPYTRGVYPSMYRGRLWTIRQFSGFGNVHQTNERYKKLIDAGQTGLSV
                     AFDMPTLMGRDSDDPRSLGEVGHCGVAVDTIKDMEILFDGLPLGDITTSMTISGPAVV
                     LFAMYLVAAERQGVAWKDLDGTLQTDIMKEYIAQKEWVFPPEPHLRLIGDLMEFCASE
                     VPKYHPISISGYHIREAGSTAAQELAFTLADGFCYVELGKERGLDVNVFAPQLSFFFD
                     AHIDFFEEIAKFRASRRIWARWLRNRYGATEPKAQQLRFHTQTAGVSLTAQQPYNNVV
                     RTAIEALAAVLGGTQSLHTNALDEVLALPSEEAAAIALRTQQVIGYETGAANVIDPLG
                     GSWFVEKFTNTMEERAEDYFRRIEALGEGGMMDGVLRGIEQGWFQQEIADAAYRYQML
                     VEKKRKIVVGVNEFESQDEKELEILRISAEVERDQITTLQRVRSERDQAAVGKALDAL
                     RSASRGSDNLMPLIIDAARAEASEGEIIGAMRDVFGEFREAPRF"
     gene            7969..8613
                     /locus_tag="VM841_11015"
     CDS             7969..8613
                     /locus_tag="VM841_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90752.1"
                     /translation="MARHMRPKLITLETLRAVDREYRDLVMSLRALAALLRDPEPAAD
                     ESKAWEPVLAGILDAELNQLATLMGDVTTALRVEHAATGPRRTIDLTRALESAVRRTG
                     SRVLIRMCEPLRVAAHPEIVSQAICSAVSLALRTADGRVSAVCRRLPDEGGVAITIPM
                     GEETHWNRFDARLGLLRRIVGAEGGRVSLEHKRDAVVLSLWFPLTGKARKRESA"
     gene            complement(8629..9138)
                     /locus_tag="VM841_11020"
     CDS             complement(8629..9138)
                     /locus_tag="VM841_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90753.1"
                     /translation="MPIFGRGEARTRMRALERAGAHRQQGDAAAAVDELAPLLVEHPD
                     DPAANVEMARSLQLLGDLAGAEEHYRRSLRVTLSYPIVVELAGVIGSLGRFQEADETL
                     DAALLMCEKDPTLHPGEAHLMRAMLAAGSGRRDDAEAALSKLDDSKPSDELRVYARRV
                     RERLDATAG"
     gene            9225..10010
                     /locus_tag="VM841_11025"
     CDS             9225..10010
                     /locus_tag="VM841_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL90754.1"
                     /translation="MGEFVNLTVDAATGVGIIRLDRPPMNAINDAVVREFEIVAREVT
                     FEDKVRAVVLTGGDKIFAAGADIKEMADRTPAEMMNKMGALQAAFTAIEEIPKVVIAA
                     INGYALGGGCELALACDLRVMAENGHIGQPEILLGIIPGAGGTQRLPRLVGPARAKDL
                     IFSGRFLKAYEAQQIGLVDLVVPAEETIQAATEMAERYAKGPTVAIRAAKIAINRGLQ
                     TDMQNGLLIERELFVNLFATEDQKAGMQSFMEHGPGKATFSGR"
BASE COUNT         1669 a         3459 c         3512 g         1487 t
ORIGIN      
        1 ttcccggcgc cgcgccgccg tggccgtccg atgacggacc gccgccgctg ccggacggag
       61 cgaccgagtc cgaacccgcc gcgttgccgc cggtgccttg gcactggctg gagtccttcg
      121 cggtcttcat gctcaccttc gtgaccgcgg ggttgcttgc ggtcgtggtc gctgccgcgt
      181 tctcgggcga cctcgaaaag gccatggccg tggtggtgtt cgaggtcgcg ctcgccggct
      241 gggcgctgct gtggccgaag atccggttcg gcctcggccc ccgtgcgctg gggttacggc
      301 gcgaccagat gggcggcgac atccggtacg ggctcggcat cggcgtggtc ggctgggtcg
      361 tgtccacgct cgtcgtggga aatgtcgtcg tccgactcgt ggagcggatt acggacggcc
      421 cggtgaccga gccggatcag ctcaactact ccgaccccag cggcctggtc ctgaccatca
      481 ccgccatcgg ggtcatcgtg atcgccccca tcgcggaaga gatcttcttc cgcggcttcg
      541 tcttccgcgg gttccgccgc gacgtcggct tcgtgatcgc cgccgtcgcg tccagcatcc
      601 tgttcatgtt cgcgcacttg cctttctgga tcatcttccc gtccatcttc accctcgggc
      661 tcttcctgtc ccgcgcgctc gagaaacggg cgtcgctgat cccgtgcatc gtcgcgcacg
      721 cgttgttcaa ctcggcgggc ttcgccgcct acatgatcac gctgaattga ccctcgccgc
      781 gaccgccgtc acgccagtgc gatcagcaac cgcacgagct cgcccgggcg ggacaccatc
      841 gggcagtgcc cgccgtcgat cgtgatcgcc tcggtgccca gccgttcgga cgcgacgtgc
      901 gagagccatg acgggctgac gagccggtcc tggcggcagg cgacgaccgt ggcgtccacc
      961 gacggcatcg catccagcgg gcacggctcg cgcaggatct tccactgctg cggccgcatc
     1021 aaagaatatg cccactgccg ccgcgccggc gatgcgtcgt ggaagtagac ctcggccgcc
     1081 cgcgccgggc cgacgctcgc ggacccgtcc tcgctcgcca aggaatgggt ctgcggcacg
     1141 tacggcgcgg cgatccgggg atcatgccag gacacgtcgt cgaacgagcg gccgggctcg
     1201 ggcacgagag ccgcgaggta gacgatccgg cgcaccggcc gcagggtggc gacgaccggc
     1261 gcggtcaggc cgcccatcga atgggccacc acgaccagat cgcccgtatc gcccgcagcc
     1321 gcggcgacga gccgcgcgta ctccgcggct ccggcggcgg ggtcggtcgc gggcagatcc
     1381 ggggcgacgg tccggtgtcc cgccccgcga agacgctcgg caaccacggc gaaatgagcg
     1441 ccccgcatga acgctccgtg tacgaacgcg aaggtggcca tcggagccgc aactctagcg
     1501 agccggtgcg atgttgagcc ttgttcacgg cgtgtgacag aatcgcggcg accgcgagtg
     1561 gggaggagac cgcatggccc tttttccgag cgaagactgg atgaagctct acgtcgagaa
     1621 gatcaactcg tcgaaggact acgaagaggc cgcgtcgacg tgggaaggcg acatcgccta
     1681 cgtcttcgag gcggaaccgg acaagggcgt gccgaacgac gtgtggtcct gggccgacct
     1741 gtggcacgga aagtgccgcg actccaagtg ggacgtgacc cccgaagaag gagagaaggc
     1801 caagttcatc atccgcgccc catactcccg ctggaaagag gtcatccgca aggagctcga
     1861 tccggtcaag ggcatgatgc aggggaagct caagctcaag ggcgacctcc cgacgatcgt
     1921 gcgctacgtg aaggcgtcca acgagctggt gaacctcgcg tcccaggtcc cgaccgagtt
     1981 cgtcgacgag caatagtgga atacgtcacc acggatcccg agatcgacgg gctcggcgcg
     2041 atgctggccg atcttgttcg cgccaacatt gccgcgcacc cggagcgggc gaagctgctc
     2101 gagaacgtca gtggcacgat caacgtcaag gcgatcgacg cgggcgtcgc ggtcgggctg
     2161 gagttcgcgg cgaaccggtt caacgtgttc gccaagccgt tccggcgcgc gggcctggag
     2221 atcatcaccg actccgggac cctgatggag cttacggccg tgcctctgtg gaagggccag
     2281 ccggacgtgc gcaccccgga gggacggacc gtccttcgca agatggtgcg acgcgacctt
     2341 cgcatcaagg gcgcagtcgc ccacccgatg ctgctcaacc ggctccagcg attgctgtcg
     2401 gcggtatgag cggcgcgcgg gtcgtggcct tcgtcggcgg gaccggcccg gcgggcttcg
     2461 gccttgcggc ccgcttcgcc aagggcggcg tgccggtcgt gatcggctcg agaagcgttg
     2521 agcgcgccga agaggcccgc acgcaggtgc tggagcgggt cccggacgcg gactgccgcg
     2581 ccatgctcaa cgaggatgcg atcgcggcgg gtgacatcgt gtttctgacc atcccgtttc
     2641 aggcgacgcg tcacaccgtc gaaacgctcg ccggccacct cgccggaagc atcgtggtgt
     2701 cgatggccaa ccccgtgtgg gtgcacgcgg ggaaggtgcg tgccgacacg cctccggcgg
     2761 gctcgctggc cgaagaggtg caggagctgg tcccgtccgc gcgtgtcgtc ggcgccttcc
     2821 acgaggtgaa cgtgcgcaag ttcccgctga tcgacaagcc gatcaacagc gacaccatcg
     2881 tcacctccaa cgatcccgag gccaagcgca tggtgatgga tctggtccgc cacgtggacg
     2941 gcatgcgggc ggtcgacggg ggagagctgc tgaatacgcg ctacgtcgaa gggttcgtgt
     3001 gcgtgctggt cacggcgaac ttcatctaca aagcatcgtc cgcgctgcgg ctgaccggtc
     3061 tgccggagga gcagtgagac gcgggctcgt cgtcgcgctc gtggccgcga ccgcgtgcac
     3121 gccggcggtt tctcccgaga gcggtcccga cccggttccc accatcagcg gcagtcccgc
     3181 tccggcgacg ggcctggccg cgctgctgtt cgacgaggcg ccgcccgggt acgtggtcgt
     3241 gccggaagag acccgcgccg tcacgctcga cgaagccgtc gaaggctccg acgatcccga
     3301 cgaggcccgc gcccggctct ccacccacgg cttcgtctcc ggcttcaccc gaacctggac
     3361 caaggacgac gacgccggca acgtcatcta cgcgttcgtc tacgagttcg ccaccgagga
     3421 aggcgcgcag gccgatatga acgccggcct cggcgaagca cgccgcgacg gcagcgaaac
     3481 cttcgacgtg cccggcatcg aaggcgcgat cggcgtgacg aaggcggccg cggccggcgc
     3541 cgaaggcgac gacagcactt tccacgccgt tttcttcgtg cgcggcaagc ggctctatct
     3601 ggtcgcgatc ggcggtccca ccgaaccgtc ccatgagcag gccgtcgagc tcgcgaagag
     3661 gatggacgtg agggcgcgct aagcgacgag ccgcgaaacg acgagcgcga gcgcaagcag
     3721 gggcagagcg aagctcagcg cgcgcagtgc ggcctcgggc gggcggcgca gccagtcggc
     3781 gtcgatgagc tcgaccgacc cgtcgcttcg ctcgacgtgg ccggtcaccg agcgggtcct
     3841 gcggcgcagc gcgcgaaccg gggtggacag cgttcgctgc gcgagcgaca gcgcgaagca
     3901 cgcggcggcg gcgccggccg cgggcagggt cggtcgcagg tcgctcgcga acgaagcggt
     3961 cagggcgggg aaggcgcccc atgccagcgc gaaccagcga ttcgaatgga agcgtccgcc
     4021 gaaccactcc aggttgtagg cggccacgac gaatccgccg aaggccacga gcgccagcag
     4081 cgtccaccgg gttcgagccg cacccgcgat gccgatcccg catgccaggc cgatcgacac
     4141 cgcggccagc acccacagcg cgcgtgacgg gatctgcgtg cgaagcggcc gtccgttcag
     4201 ctcgtcgagc gcgtgcgcgc cgatgccgac cgccaggaag aatgcgatca gcagcgccac
     4261 cagccggtcc acgtgcacgg tctcggccac ggaccatccg atcgcgacgt agctcagatg
     4321 ccaggccgtg tacggcggat gcagcagcgt gacgtagtcg cgccacccgc cggttcgcag
     4381 cgcgtagaac gcgggcgcgt ccggcttacg gagcggctcg gccatctttg cgtcccatca
     4441 tcagcacgcc cgtcccgaac gtcatccgtt tgatgcgcac gtccgtcagc cctgcgtcct
     4501 gccacagcac cgcctgcttt tccggtggca gcgacgcatc cagttccatg atgctgccgg
     4561 gaaggaactg tcccgtccac ccccagcctg gggagacgat ccgagccgcc catgggaaca
     4621 cgccgcgcga atagattctc cagcccaggc gcggcagccg cgcgcgcggg acgccgaact
     4681 cgaccgatcc catgacgccg ccgggcctga gcacccgagc catctcggcg atcgtggccg
     4741 ccggatcgtc cacgtagcga agcagatacg agaagacgac cccgtcgaag gtcgcgccgg
     4801 cgaacgggag ccgctcggcc tgccccgcga cgcgcgcgcc cgctccgcgc gtcagcatcg
     4861 gcaggctctg gtccagcccg gtcacgcgtg cccccgcgat ctgctccaga tcgcgcgaga
     4921 tcaggccggt tccgcacgcg acgtccaaga ccgcatcgcc gtccgccagc ggcagagccc
     4981 ggaccagcgc ccgccgccat cgaccgtact cgccgaagga cagcacggcc gccggccggt
     5041 cgtaggcggg cgcgatcccc gcgaacaacg ctcgcgcccc ggccgaccgc tcgctggcca
     5101 catcttcggt tttacctcga cgggggtgac cgacgaaagt gcgccccgcc cggcgacggt
     5161 cgggcgcccc cgccgtgcgc ccacggtacg atgagccttc tatggacgta tccgccctgg
     5221 tcgagggtct ccgaagcggc gatcgccgga cggtcgcgcg catgatctcg ctggtcgagg
     5281 acgcctcgat cgagacgctc ggggaggtga tgcgccagat cggcggcatg acctcccacg
     5341 cctacacgat cggggtcacc ggctcgcccg gcgtgggcaa gtccacgctg accggcgcgc
     5401 tggtcagcga gcttcgtaaa cgtgaccgga ccgtcagcgt gctggcgatc gatccgacat
     5461 cgcacatcac gggtggcgcg ctgctggggg accggctgcg gatgcaggag cacgcgaccg
     5521 atccgggcgt gttcatccgc tcgatggcca cgcgcgggca tctgggcggc ctggcctggg
     5581 cggccccgca cgcgctgcgg atcctggacg cggccggggt ggacacgatc ctggtcgaga
     5641 ccgtcggcgt cgggcaggac gaggtcgaga tcagcgcgca ggccgatacg accatcgtcg
     5701 cgctcgcccc cggcatgggc gacagcgtgc aggccaacaa ggccggcatc ctcgagatcg
     5761 ccgacgtgta cgtggtcaac aaggcggacc gcgagggcgc caagcaggtc gtgcgcgaca
     5821 tccagcagat gctcgagctg ggcgctcacg gcgaatggct gccgccggtc ctgatgacga
     5881 ccgcgaccga cggcaccggc gtggacgagg tcctgaatgc ggtcgaccgg caccgggaat
     5941 acatggactc gtcgggcgag gcgcggcgcc gccgcctggc gcgcgccgcg ggcgagatcc
     6001 gcgagatcgt gatcggacgc atgcgggccg cgatcggcac cgtgaaagat tcggacctac
     6061 tggccgcgct tgccgagcag gtggtcgagc acgaactcga tccctacgcg gcggcggata
     6121 aactgctcgc agaggtggac gcatgaccga gggtgcatac gagcaatggc gcgcggccta
     6181 cgaggccggg ctcaaacgcg aggcagactt cgacacgctc tcgggcgagg tgctcgagcc
     6241 gctctacacg ccggcagacg tcgaagacat cgactacgaa accgacatcg gcctgcccgg
     6301 tcagttcccg tacacgcgcg gcgtctatcc gtcgatgtac cgcggacggc tgtggacgat
     6361 ccggcagttc agcggattcg gcaacgttca ccagaccaac gagcgctaca agaagctgat
     6421 cgacgcggga cagaccggct tgtccgtcgc gttcgacatg ccgacgctga tgggccgcga
     6481 cagcgacgat ccgcgttcgc tgggcgaggt cgggcattgc ggcgtcgcgg tggacacgat
     6541 caaggacatg gagatcctgt tcgacggact gccgcttggt gacatcacca cctcgatgac
     6601 gatcagcggc cccgccgtcg tgctgttcgc gatgtatctg gtcgcggccg agcggcaggg
     6661 cgtcgcctgg aaggatctcg acggcacgct gcagaccgac atcatgaagg aatacatcgc
     6721 gcagaaggaa tgggtgttcc cgcccgagcc gcacctgcgt ctgatcggcg acctgatgga
     6781 gttctgcgcg agcgaggttc ccaagtacca cccgatcagc atcagcggct accacatccg
     6841 cgaagccggc tcgaccgctg cgcaagaact ggcgttcacg ctggcggacg gcttctgcta
     6901 cgtcgagctg ggaaaggagc gcggcctgga cgtcaacgtc ttcgcgccgc agctgtcgtt
     6961 cttcttcgat gcccacatcg acttcttcga agagatcgcc aagttccgcg cgtcgcgtcg
     7021 catttgggcg cgctggctcc gcaaccgtta cggcgccacc gagccgaagg cccagcagct
     7081 tcgcttccat acacagaccg ccggcgtgtc gctgaccgcg cagcagccgt acaacaacgt
     7141 ggtgcgcacc gcgatcgagg cgctggccgc ggtgctgggt ggcacgcagt cgctgcacac
     7201 caacgcgctc gacgaggtgc tcgcgctccc cagtgaagag gccgccgcca tcgcgctccg
     7261 cacccagcag gtgatcggat acgaaaccgg cgccgccaac gtcatcgacc cgctcggcgg
     7321 atcctggttc gtcgagaagt tcaccaacac gatggaggaa cgcgccgagg actacttccg
     7381 ccgcatcgag gcgctcggcg agggcggcat gatggacggc gtgctgcgcg gcatcgagca
     7441 gggctggttc cagcaagaga tcgccgacgc cgcctatcgc tatcaaatgc tcgtcgagaa
     7501 gaagcgaaaa atcgtcgtcg gcgtcaacga gttcgagtcc caggacgaaa aagagctcga
     7561 gatcctgcgc atcagcgccg aggtcgagcg cgatcagatc acgacgctgc agcgcgtccg
     7621 gtccgaacgt gaccaggccg ccgtgggcaa ggccctcgac gcgctgcgct cggcatcgcg
     7681 cggcagcgac aacctgatgc cgctgatcat cgacgccgca cgcgccgagg ccagcgaagg
     7741 cgagatcatc ggcgcgatgc gggatgtttt cggggaattc cgggaggcac cccgcttcta
     7801 ggcgatcgac ccgattggtt gctccgttcc gctttgccgg gatgattccg tgccccgtcc
     7861 gctttgccca cgagtgcgct ctgttgccgg acggccacgc agggtaatac tggaggggcc
     7921 gcccggcccg tccggttccc gtctggagag caaggggtgt tgcaccgcat ggcccgccac
     7981 atgcgcccga agctgatcac gctcgagacc cttcgagcgg tagatcgcga gtaccgcgat
     8041 ctcgtcatgt ccctgcgcgc gctcgccgcg ctgctccgcg atcccgagcc cgccgcggac
     8101 gagagcaagg cctgggagcc cgtgctcgcc ggcatcctcg acgccgagct caaccagctc
     8161 gcgacgctga tgggagacgt caccaccgcg ctgcgcgtcg agcatgctgc gaccgggccc
     8221 cggcggacca tcgacctgac gcgcgccctg gagtcggcgg tgcggcgaac cggctcgcgt
     8281 gttctgatcc ggatgtgcga gccgttgcgc gtcgcggcgc atcctgagat cgtctcgcag
     8341 gcgatctgca gcgccgtctc gctcgcgctg cgcaccgccg acgggcgcgt gtccgcggtc
     8401 tgccgccggc tgccggacga agggggcgtg gcgatcacga tcccgatggg cgaggagacc
     8461 cactggaacc gattcgacgc ccggctgggg ctgctgcgcc gcatcgtcgg cgccgaaggc
     8521 ggccgggtct cgctcgagca caagcgggac gcggtcgtgc tgagcctgtg gtttccgctg
     8581 accggaaagg cgcggaaacg cgagagcgcc tagaccggtc gagtcccatt agccggccgt
     8641 cgcgtcgagc cgctcgcgca cgcgccgtgc gtacacgcgc agctcgtcgg agggcttgga
     8701 gtcgtcgagc ttcgacagcg cggcctccgc atcgtcgcgg cgtcccgagc cggccgcgag
     8761 catcgctcgc atcagatgcg cctcgccggg atgcagcgtc gggtccttct cgcacatcag
     8821 cagcgctgcg tcgagcgtct cgtccgcttc ctggaaccgg ccgagcgagc cgatcacgcc
     8881 ggcgagctcg accacgatcg gataggagag cgtgacccgc agtgatcgcc ggtaatgctc
     8941 ctccgcgccg gcgaggtcgc cgaggagctg caaggaacgg gccatttcga cattggcggc
     9001 cgggtcgtcc gggtgctcga cgagcagcgg cgcgagctcg tccaccgcgg cggcggcgtc
     9061 gccctgctgg cggtgggctc cggcgcgctc gagcgcacgc attcgcgtgc gcgcctcgcc
     9121 acggccgaag atgggcatcg cggggcatct tatagaatcg ccccgctgtc cgccgacgcc
     9181 ggccgtgcgt ggcgaacgac gaaacacgag gaaaggggac cgtggtgggc gagttcgtga
     9241 acctgaccgt ggacgccgcg accggcgtcg gaatcatccg gctggatcgt ccgcccatga
     9301 acgcgatcaa cgacgccgtc gtgcgcgagt tcgagatcgt tgctcgcgag gtcaccttcg
     9361 aggacaaggt tcgcgcggtg gtgctgacgg gcggcgacaa gatcttcgcg gccggcgccg
     9421 acatcaaaga gatggccgat cgcacgcccg ccgagatgat gaacaagatg ggagcgctgc
     9481 aggccgcgtt caccgcgatc gaggagatcc caaaggtcgt catcgccgcg atcaacggct
     9541 acgcgctcgg cggggggtgc gagctcgcgc ttgcctgcga cctgcgggtc atggcggaga
     9601 acggtcacat cggccagccc gagatcctgc tcgggatcat cccgggagcc ggcggaacgc
     9661 agcggctgcc gcggctcgtc gggccggcgc gcgcgaagga tctcatcttc tcggggcggt
     9721 tcctgaaagc ctacgaggcg cagcagatcg gcttggtgga tctggtcgtg ccggccgagg
     9781 agacgattca ggccgcaacc gagatggccg agcggtacgc caagggcccg actgttgcca
     9841 tccgcgcggc gaagatcgcc atcaaccgtg gtttgcagac ggacatgcag aacggtcttt
     9901 tgatcgagcg cgagctgttt gtgaacctgt tcgcgaccga ggatcagaag gcgggaatgc
     9961 aatccttcat ggagcacggg cccggcaagg cgactttctc cggccgctaa tagccgactg
    10021 acagacgggc atgatcgttc acccccaatt gggggccggt ccgtccgcgg cgcggcatgg
    10081 cccggcagga ggatggacac gggtgccgtt tcgccctact ctcgggc
//
LOCUS       DATLFO010000268         7871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_65181, whole genome shotgun sequence.
ACCESSION   DATLFO010000268 DATLFO010000000
VERSION     DATLFO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65181"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VM841_11030"
     CDS             <1..1524
                     /locus_tag="VM841_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVL90755.1"
                     /translation="TREQLGVALERQRETGQFLGKILLEMGLVDEGTLVEAVARQIGV
                     PFIDLTQRRPTADVLAMVPAEMARKHAVVPAALEGNTLILAMAEPTNVAAVREIAAMT
                     GLEVKPALAVRRHIHATLDGAAGAVAPSAPTLEEEFLRETSPAIPAAEEVKEIAMHDL
                     LERTLVEGASDLHLTAGVPPTIRVNGRLLALHEYPILHPVEIRRLAYSVLTQRQREKL
                     ENDLELDLAYTLPGRARFRVNVYFQRDAIGAAFRLIPTSIRTVRDLGLPTRVAEFARL
                     ARGLVLVTGPTGHGKSTTLAALIDLINTERNEHIMTIEDPIEYLHQHKASLVNQREVG
                     SDTKSFAGALRHVLRQDPDVILVGEMRDLETIQTALTAAETGHLVFATLHTQDAPQTI
                     DRIIDVFPPHQQQQVRVMLAASLQGIVTQQLLPTQNGSGRAVACEVLVATPAIRNLIR
                     EGKSHMIYSALQAGAKFGMQSMDQSLGELVRLGKISYQLGLERAHNAEDFNKLSGRG"
     gene            1533..2753
                     /locus_tag="VM841_11035"
     CDS             1533..2753
                     /locus_tag="VM841_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL90756.1"
                     /translation="MPAATYAYKVRDKQGHLVTGQLEADNQAAVAAKLRQMGYSPILI
                     EEQKTTGVKREVKLPWKNGVKGKDLAVFSRQFATMINSGLSLLRALNILADQTENQTL
                     AKVVGEVRSDVERGASLSASMAKHPKIFSRLYVSMIRAGEIGGVLDSVLLRLAENLEK
                     EVALKQKIKSAMTYPVVVFIMVIMIVSGMLLFVVPMFENLYKDLGGKLPLPTQLLINV
                     SNIMKKMWFLVIGAGIGAGIAFKRWIVTDKGRASWDAVKLKAPIFGDLVHKTALSRFS
                     RTLGVLIRSGVPILQSLDIVKETSGNAVVGKATQDVQRSVKDGESIAKPLERHAVFPA
                     MVVQMIAVGEETGALDTMLEKIADFYDQEVEATVDALTSLIEPLLIAVMGMVVGGMVV
                     ALYMPMFNIINLIQ"
     gene            2980..3477
                     /locus_tag="VM841_11040"
     CDS             2980..3477
                     /locus_tag="VM841_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVL90757.1"
                     /translation="MLIAIRKRLQDKEEGFTLIELMVVVLIIAILIAIAIPTFLGARK
                     RAQDRAAQSNIRNAFTAEKTIYTDAQAYAAHDDDLDGSGSADLAEIEPSLTFVNTDPP
                     TATGNTVSIVLVGSTVYVMGKSASGACFYLRDNATGATEYAKDTANSPCLASGGALTW
                     STTPW"
     gene            3622..4116
                     /locus_tag="VM841_11045"
     CDS             3622..4116
                     /locus_tag="VM841_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVL90758.1"
                     /translation="MACQFSGDDVLDLIAVRGPAPNERGFTVIELLVVSLIIGILIAI
                     AVPAFAGAQQRANDRSAQTLLRHGLAAQKIVYTEILRYADDSAGALTGIEPGLVYESG
                     MTPSTPGRVAVGLSGNDIVYLSGKSKTGTCFYLVDDALSGTGYAQDDLCGAADTQSYV
                     TNGW"
     gene            4238..5491
                     /locus_tag="VM841_11050"
     CDS             4238..5491
                     /locus_tag="VM841_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90759.1"
                     /translation="MFRRFRLRASRGQEGIALVELTGALTILAIGFLALAAGLGGGFR
                     QIALSRQRQVASEIGNGRVEHLRNVPYAYVAVSSDVTHNTDPSHPDHFVDDATNSFDY
                     SGTGTYEPLIEDPSIGQVEHFESPVVVGATTLTVYQYVTWVDDPAIDGTEDYRRVTVV
                     VQFNRPAAEGAARMVRVSSYFTSGNVTLGGTTPGVATGSPTPSGSASPSPTPSGSCSG
                     DTTAPSGSFEIFGGIASETGFTASRSVTLPFSGVSDPCTPIRWRFSNDDVTYGDDIVY
                     DASNPSVAWALAGADGTKQVWGRLRDGLGNERTVGPRSVVLDTTKPTVPGTLSRTVAC
                     AGANRTVNLSWGSSTDTNFRGYRIYKRIDGGAWFELRTVSSTSSSDTDKKSYDSLQYY
                     VVGYDKAGNQSNGTNIVSLAKNQCS"
     gene            5469..5957
                     /locus_tag="VM841_11055"
     CDS             5469..5957
                     /locus_tag="VM841_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HVL90760.1"
                     /translation="MRKTSAAERGFTIVELSVTVSIFAIVLTALLGVFISVQRSQAYV
                     TDRSESMDQLRIALDRVSKEVRQATTVTTKTPTSIAMSTYVAGAAEVVTWSVTGGVLR
                     RTNEWGSTTTLLREITNTDVFTYTPATGNPLVVTVRLEMRPRHSPNVRLELTSEIRLR
                     NG"
     gene            5954..7384
                     /locus_tag="VM841_11060"
     CDS             5954..7384
                     /locus_tag="VM841_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90761.1"
                     /translation="MINLRVLPVRRDDDGFVMIVSVLLLSVIASLGVVVMTSGNHSSQ
                     ATGRGRSWVQSLHVAEAGIQDAMARMDATGSPPATVTGSTHEGTYTVTVTPLGRSRYL
                     VESLGTVAAGSSMKAARRVQVTLAPPRSFDAALFSYTSVDTKNNDLVVGDIWANQNVI
                     VEANDIVQGSVTAATGFVQLKNGSRVTKNVQTGGYNPATTNGIFLDNNARIDGDATAS
                     VTAPTDPVTCGGESQNQYTIRMQTGSVIAGNAKTWGSRTGSGTVSGSVTEHLCTAAPA
                     ARPLPIFSYNAANYDAATLREWGTPSSPNPTARADFNTYLAANKNSMQGTFVVFQSGT
                     ITQQSRLDLTGVKIAGDFALITNLPIFTNGIEDLASVTDAIALFASFYQPPAGSVCDV
                     NHDNSDCSIHAKNNFRTSGATASLMYAPYGPVAIKNNQEMFGAVYADNIQIKNNQTLT
                     YDRRVDRLVGFGPVTLEPTDWMELAP"
     gene            7425..>7871
                     /locus_tag="VM841_11065"
     CDS             7425..>7871
                     /locus_tag="VM841_11065"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HVL90762.1"
                     /translation="MDAFFVAIAALFGLLFGSFLGLVVYRVPRRESILRPRSRCTNCG
                     YELKPSDNIPVLSWVMLRGRCRSCRTPISARYPAAEVLTALLWAIAVARLGVCDGCRP
                     MDAIAFLPFLWVLVALSLIDLEHKLIPSRILYPFLGAGIVLIAVAAA"
BASE COUNT         1420 a         2695 c         2473 g         1283 t
ORIGIN      
        1 acacgcgagc agctcggcgt cgcgctcgag cgacagcgag agaccggcca gttcctcggc
       61 aagatcctgc tcgagatggg cctggtcgac gaaggaaccc tggtcgaggc ggtcgcccgc
      121 cagatcggcg tgccgttcat cgatctgacc cagcgccgtc caacggccga cgtgctggcc
      181 atggttcccg ccgagatggc ccgcaagcac gcggtcgtgc cggcggcgct cgaaggcaac
      241 accctgatcc tggccatggc cgagccgacg aacgtcgcgg ccgtgcgcga aatcgcggcg
      301 atgaccgggc tcgaggtcaa gccggccctc gcggtgcgac gccacatcca cgccaccctc
      361 gacggagccg ccggcgcggt tgcgccgtcc gcgccgacgc tggaagagga gttcctccgc
      421 gagacgtctc cggcgattcc cgcagcggag gaggtcaagg agatcgcgat gcacgacctt
      481 ctcgagcgca cgctcgtgga aggcgcgtcg gaccttcact tgaccgccgg ggtgccgccg
      541 accatccgcg tcaacggccg gctgctggcg ctgcacgagt atccgatcct tcacccggtg
      601 gagatccgcc ggctcgccta ctcggtgctc acccagcggc aacgcgagaa gctcgagaac
      661 gacctcgagc tcgatctggc gtacacgctg ccgggacggg cgcgcttccg cgtcaacgtc
      721 tacttccagc gcgatgccat cggcgcggcg ttccggctga tccccaccag catccggacg
      781 gtcagggatc tcggccttcc cacgcgggtg gcggagtttg cgcggctcgc gcgcgggctg
      841 gtgctggtga ccggccccac cggacacgga aagtccacca cgctcgccgc gctcatcgac
      901 ctgatcaaca ccgagcgcaa cgagcacatc atgaccatcg aggatccgat cgagtacctc
      961 caccagcaca aggcgtcgct ggtgaatcag cgcgaggtcg gctccgacac gaagtcgttc
     1021 gcgggcgccc tgcggcacgt gctgcgtcag gatcccgacg tgatcttggt tggcgagatg
     1081 cgcgacctcg agacgatcca gacggctttg accgccgccg agaccggcca tctggtcttc
     1141 gcgacgctgc acacccaaga cgcgccccag accatcgacc gcatcatcga cgtgtttccg
     1201 ccccaccagc agcagcaggt ccgggtcatg ctcgcggcct cgctgcaggg gatcgtgacc
     1261 cagcagctgc tgcccacgca gaacggcagc ggccgcgcgg tcgcgtgcga ggtgctcgtc
     1321 gccacgccgg cgatccggaa cctcatccgc gagggcaaga gccacatgat ctactccgcg
     1381 ctgcaggcgg gcgcgaagtt cggcatgcag tcgatggatc agtcgctcgg cgagctggtt
     1441 cggctcggca agatctcgta tcagctcggc ctcgagcgcg cgcacaacgc cgaggacttc
     1501 aacaagctca gcggacgggg gtagagggac gcatgccggc cgcaacctac gcatacaagg
     1561 tccgcgacaa gcagggccat ctcgtcaccg ggcagctcga ggccgacaac caggctgccg
     1621 tcgccgcgaa actgcggcaa atgggctaca gcccgatcct gatcgaggag caaaagacca
     1681 ccggcgtcaa gcgcgaggtc aagcttccct ggaagaacgg ggtcaagggc aaggatctcg
     1741 ccgtgttctc acggcagttc gccaccatga tcaacagcgg cctgtcgctg cttcgggcgc
     1801 tgaacatcct ggcggatcag accgagaacc agacccttgc caaggtcgtc ggcgaggtgc
     1861 gctccgacgt ggagcgaggc gcgtccctgt cggcctcgat ggccaagcat cccaagatct
     1921 tctcgcggct gtacgtgtcg atgatccgcg ccggcgagat cggcggtgtg ctcgacagcg
     1981 tgctgctgcg gctcgccgag aacctcgaga aagaggtcgc cctcaagcaa aagatcaaga
     2041 gcgcgatgac ctacccggtc gtggtcttca tcatggtcat catgatcgtg tccggcatgc
     2101 tgctgttcgt cgtgccgatg ttcgagaacc tctacaagga tctcggcggc aagcttcccc
     2161 tgccgaccca gcttctgatc aacgtgtcaa acatcatgaa gaagatgtgg ttcctggtga
     2221 tcggagccgg gatcggcgct ggcatcgcgt tcaaacgatg gatcgtcacc gacaaaggac
     2281 gcgcgtcgtg ggacgcggtc aagctgaagg cgccgatctt cggggacctg gtccacaaga
     2341 ccgcgctgtc gcgcttctcg cgcacgctgg gcgtcctgat ccgctcgggc gtgcccattc
     2401 ttcagtcgct cgacatcgtt aaggaaacct cgggcaacgc ggtggtcggc aaggcgacgc
     2461 aggacgtgca gcgttcggtt aaggacggcg agtcaatcgc caagccgctc gagcggcatg
     2521 cggtgttccc ggcgatggtc gtgcagatga tcgccgtcgg cgaggagacc ggcgcgctcg
     2581 acacgatgct cgagaagatc gccgacttct acgatcaaga ggtcgaggcg acggtcgacg
     2641 cgctcacgtc cctgatcgag ccgctgctga tcgcggtgat gggcatggtc gtcggcggca
     2701 tggtcgtggc cctgtacatg ccgatgttca acatcatcaa cctgatccag tagcaagaag
     2761 gctcctgcgg cggcccccgg accggcggcc gccgccccac gttcggagcc cggccggcgt
     2821 gacgtatagc ccgcctctag ccacgcgtga cggttctgat ccggcaagcc cggcgaatcc
     2881 cgaaattccc ctcgtcatta cctcaagtct cttgctgctg cgcccgatat caggcgggag
     2941 aagcagcaca gcccctcagg ggcagaaagg agagcagtaa tgctcatcgc cattcgcaag
     3001 cgcctccagg acaaggagga gggcttcacg ctcatcgagc tcatggtggt cgttctgatc
     3061 attgccattc tgatcgcgat cgcgatcccg accttcctcg gagctcggaa gcgtgcgcag
     3121 gaccgtgcgg ctcagtcgaa catccgcaac gcgttcacgg ctgagaagac gatctacacg
     3181 gacgcgcagg catacgcggc ccacgacgac gacctcgacg gaagcggttc ggccgacctg
     3241 gccgagatcg agccgtccct gacgttcgtc aacaccgacc cgccgacggc gaccggcaac
     3301 acggtctcga tcgtgctcgt cggctccacg gtctacgtca tgggcaagtc cgcgtccggc
     3361 gcatgcttct acctgcggga caacgccacg ggagcaaccg agtacgcgaa ggacacggcc
     3421 aacagcccgt gccttgcgtc aggcggcgcg ctcacgtggt cgaccactcc ctggtagatc
     3481 gcgcagcagt tgggacgagg gcgacattgg tcgccctcgt ctcctgttct ccccccgcga
     3541 acggcggtcc gccgcgggcc acctcgtcca cttcggattc tcgaccaccc tgccgataat
     3601 cagaagaggc agccagccac cgtggcttgt cagttctcgg gagacgacgt tttggacctg
     3661 atcgcagttc gaggacccgc gccgaacgag cgcggtttca cggtgatcga gctcttggtc
     3721 gtctcgctga tcatcgggat cctgatcgcc atcgcggtcc cggcattcgc aggagcccag
     3781 cagcgagcta acgaccgaag cgcccagacc ctgctgcggc acggactagc ggcgcagaag
     3841 atcgtctaca ccgagatcct tcgttacgcc gacgattccg ccggggccct gaccgggatc
     3901 gagcccgggc tcgtctacga aagcggaatg accccgtcga cgccggggcg tgtcgccgtc
     3961 ggcttgtcgg gcaacgacat cgtctatctg tccggaaaga gcaagaccgg aacgtgtttc
     4021 tacctcgtgg acgacgcgct ctcgggaacc gggtatgcgc aggatgacct ctgcggagcg
     4081 gccgataccc aaagctatgt cacgaacggc tggtgacgta ggtaacacgg cgtaagttcc
     4141 ggcaaatccc caacctgggg gtcaagcgga cgcgagcgta ttccgatgct ccaaggggga
     4201 cgcatttctc gttcccggga aagggtcgaa ggtcggaatg ttcagacggt tccgtctccg
     4261 cgcgtcacgg ggccaagagg ggattgcgct ggtcgaactg accggagcgc tgaccatcct
     4321 cgccattggc ttcttggcgc tcgccgccgg cctcggaggt ggcttccgcc agatcgcgct
     4381 gtcccggcaa cgccaggtcg cgtccgagat cggaaacggc cgcgtggagc atctccgcaa
     4441 cgttccctac gcctacgtcg cggtgtcgtc ggacgtgaca cacaacaccg acccgagcca
     4501 tcccgaccac ttcgtcgacg acgccaccaa ctcgttcgac tacagcggca ccggaaccta
     4561 cgagccgctc atcgaggacc cgtccatcgg ccaggtggag cacttcgaaa gcccggtcgt
     4621 cgtgggcgcg acgaccctga ccgtctacca atacgtcacc tgggttgacg accccgccat
     4681 cgacggcacc gaggactacc ggcgcgtgac ggtcgtggtg cagttcaacc gcccggcggc
     4741 ggaaggagcc gcgcgcatgg tccgcgtttc gtcgtacttc acgtccggga acgtgaccct
     4801 cggcggcacc acgccgggcg tcgcgacggg cagcccgact ccgagcggct cagcttcgcc
     4861 gtcgccgacc ccgtccggct cgtgctcggg cgacaccacg gcaccgagcg gcagcttcga
     4921 gatcttcgga gggatcgctt ccgagaccgg cttcaccgcg agccgctccg tgacgcttcc
     4981 cttcagcggg gtctccgatc cgtgcacgcc gatccggtgg cgtttctcca acgacgacgt
     5041 gacctacggc gacgacatcg tctacgacgc tagcaatccc tccgtcgcgt gggccctcgc
     5101 gggcgcggac ggaacgaagc aggtctgggg caggcttcgc gacggcctcg gcaacgagcg
     5161 aacggtcgga ccgcgctcgg tggtgctcga cacgacgaag ccgaccgtgc cgggcacgct
     5221 cagccgcact gtggcgtgcg ccggtgcgaa ccggacggtc aacctgtcct ggggatcgtc
     5281 gaccgacacc aatttccgcg ggtaccgcat ctacaagcgc atcgacgggg gagcatggtt
     5341 cgagctcaga accgtctcct cgaccagcag cagcgacacg gacaagaaga gttacgactc
     5401 cctgcagtac tacgtggtcg ggtacgacaa ggccggcaat caatcgaacg gaacgaacat
     5461 tgtttccctt gcgaaaaacc agtgcagctg agcgtggctt caccatcgtc gagctttcgg
     5521 tgacggtgtc gatcttcgcg atcgttctga ccgcgctgct cggtgtattc atctccgtgc
     5581 agcgctcgca ggcgtacgtg accgaccgaa gcgagtcgat ggatcagctt cgcatcgcgc
     5641 tggaccgcgt ctccaaagag gtccggcaag ccaccaccgt aacgaccaag actccgacgt
     5701 ccatcgccat gagcacctac gtggccggag cggccgaggt cgtgacgtgg agcgtcaccg
     5761 gtggggttct gcggcgcacg aatgagtggg gctccacgac cacgctgctt cgcgagatca
     5821 ccaacaccga cgtcttcacc tacaccccgg ccaccggcaa cccgctggtc gtgacggtcc
     5881 ggctggagat gcgtccccgc cacagcccaa acgtcaggct cgagctgacg tccgagatca
     5941 gattgaggaa cggatgatca acctgcgcgt gcttcccgtc cgtcgcgatg acgacggctt
     6001 cgtcatgatc gtgtcggttc tgctgctgtc ggtcatcgcc tcgctcggag tggtcgtgat
     6061 gacgtcgggc aaccattcca gccaggccac agggcgcggc cggtcgtggg tgcagtccct
     6121 gcacgtggcc gaggccggga tccaggatgc tatggcgcgg atggacgcaa ccggcagccc
     6181 cccggcgacc gtgaccggat ccacgcacga gggcacctac accgtgaccg tcaccccact
     6241 cgggcgatct cgctatctcg tcgagtcgct cgggacggtc gcggccggca gctcgatgaa
     6301 ggccgcccgc cgggtccagg tcaccctcgc gccgccgcga agcttcgacg ccgcgctctt
     6361 ctcgtacacc agcgtcgaca ccaagaacaa cgatctggtc gtcggagaca tctgggcgaa
     6421 ccagaacgtc atcgtcgagg cgaacgacat cgtccaggga tcggtcacgg cagccaccgg
     6481 cttcgttcag ctcaagaacg gatcccgcgt caccaagaac gtccagacgg gcggatacaa
     6541 ccccgcgacg accaacggga tcttcctcga caacaacgcg cgcatcgacg gggatgccac
     6601 ggcttcggtg acggctccga ccgacccggt cacctgcggc ggcgagagcc agaaccagta
     6661 cacgatccgc atgcagaccg gctcagtcat cgcgggcaac gcaaagacct gggggtcacg
     6721 caccggctcc ggcaccgtct ccggctccgt gaccgagcat ctgtgcaccg cggccccggc
     6781 cgcccgtccg ctgccgatct tctcctacaa cgcggcgaac tacgatgcgg cgaccctgcg
     6841 ggagtggggc acgccctcct cgccgaaccc gaccgcccgc gccgacttca acacctacct
     6901 cgcagcgaac aagaactcga tgcaagggac cttcgtggtg ttccagtcgg gaacgatcac
     6961 gcagcagagc cgattggacc tgaccggcgt gaagatcgcg ggagacttcg cgctgatcac
     7021 caaccttccc atcttcacga acgggatcga agatcttgcg agcgtgaccg atgcgatcgc
     7081 gctcttcgcc tccttctatc agccgcccgc gggatcggtg tgcgacgtga accacgacaa
     7141 cagtgactgc tcgatccacg ccaagaacaa cttccggacc tccggcgcga cggcctcgct
     7201 gatgtacgcc ccgtacgggc ccgtcgccat caagaacaac caagagatgt tcggggccgt
     7261 ctacgccgac aacatccaga tcaagaacaa ccaaacgctc acctacgacc gccgcgtgga
     7321 ccgcctcgtc gggtttggcc cggtgacgct cgaacccacg gactggatgg agctggctcc
     7381 ttagcgtgcg agacccggca gcgccctgag gtagattccg ctccgtggac gcattcttcg
     7441 tcgccatcgc ggccctgttc ggactgctgt tcggttcgtt cctggggctg gtcgtgtacc
     7501 gggttccgcg ccgcgagtcc atcttgcggc cgaggtcgcg ctgcaccaac tgcggctacg
     7561 aactcaaacc ctccgacaac atcccggttc tgtcctgggt gatgctgcgc gggcgctgcc
     7621 gttcctgccg cacgccgatc tcggcccgct atccggccgc cgaggtcctc accgcgctgc
     7681 tgtgggctat cgcggtcgcc cggctgggtg tctgcgacgg atgtcgtccg atggatgcga
     7741 tcgcgttcct tccgttcctg tgggttctgg tcgcgctgag cctcatcgac ctcgagcaca
     7801 agctgatccc cagccgcatc ctgtatccgt tcctcggcgc cggcatcgtg ctcatcgccg
     7861 tggccgcggc c
//
LOCUS       DATLFO010000269         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88607, whole genome shotgun sequence.
ACCESSION   DATLFO010000269 DATLFO010000000
VERSION     DATLFO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88607"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VM841_11070"
     CDS             complement(<1..604)
                     /locus_tag="VM841_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVL90763.1"
                     /translation="MARQYRSAFRKALAIGAVIGAALVVPIVDALATSTYKLARMDQP
                     AAPIVEGEPMGKVRQPGKPVAVIIASNRATEVTDFLPPFQMLSASGAFNVYVVTPERA
                     PATMYTGMMRPAGLSVVPDYSFAEYDATVARAPDLIVIPYLPGFTPERDAPTLGWIRK
                     HAGPATRLLSVCAGAEILAATGLLDGHRATANLGGVSQYMN"
     gene            complement(633..1982)
                     /locus_tag="VM841_11075"
     CDS             complement(633..1982)
                     /locus_tag="VM841_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HVL90764.1"
                     /translation="MRGDTTAMTNHCTHESDKNAAADYTPLVTNPGREVVVALEARAA
                     GWDIGGGHKALDGWSYNGITPGPTIEARQGDTLVVRLTNRLDEPTTIHWHGLRLQPAM
                     DGTESVQQAVAPGEAFEYRFALPDAGTFWYHPHANETVQLERGLYGVLVVHGPDEPQV
                     DAERVLVFDDVRLDWRGRLAGPGGWLERHNGREGRVPLLNGRVRPELEMAAGQVERWR
                     LVNTASARYVRFSLGGAPFRIIGTDGGLIEAPVDATEVLLAPADRVDLLVGPFEESQR
                     LDIQSLEYNRGLGVKGTAVTFGTVRVGLSRRSVARISEKLRTIDPIAPPDAPVTREVR
                     LGGRMSLRRGVDFMVNDEPHHHGQPVRVGELQVWDVINETPLDHPFHLHGFFFQVLAV
                     NGEAPAYRSWEDTVNVPPKGRVKIAWMPDDRPGAWMAHCHILEHHAAGMMMHFEVVR"
     gene            complement(2016..>2326)
                     /locus_tag="VM841_11080"
     CDS             complement(2016..>2326)
                     /locus_tag="VM841_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90765.1"
                     /translation="GQELHVDAVVWTAGYHEETGWVDIPEAKDQHRGFIHHRGITPAR
                     GLYLLGRSWQWTRGSALLWGVGNDARFLVNHLSGQLGQPVYSSTSTAPFKRFGRLVQS
                     "
BASE COUNT          400 a          784 c          744 g          398 t
ORIGIN      
        1 agttcatgta ctggctcacg ccgccaaggt tcgcggtggc acgatggccg tcaagcaatc
       61 ccgtcgccgc cagaatctcg gcccccgcgc acacggacag cagccgcgta gccggtccgg
      121 cgtgtttcct gatccacccc agtgtgggcg cgtcgcgttc cggcgtgaaa cccggcaggt
      181 agggaatcac aatcaagtcg ggtgcgcgcg ccaccgtcgc atcgtattcg gcgaacgaat
      241 agtccggcac gacgctcaga cccgccggac gcatcatccc cgtgtacatc gtcgcgggcg
      301 cgcgctcggg cgtcacgaca tacacgttga acgcaccgct cgccgatagc atttgaaacg
      361 gcggtaggaa atcggtcact tcggtggcgc ggttgctcgc gataatcacc gcgacaggct
      421 tgccgggctg acggaccttg cccatcggct cgccctcgac aatcggtgct gcgggctgat
      481 ccatgcgcgc cagcttgtac gtcgaagttg cgagggcatc cacaatcggg acgacgagcg
      541 cggcaccgat cacggcgccg atcgcgagcg ccttccggaa ggccgagcga tactgccttg
      601 ccatggcaat cccctccctc atcggcattc gattaccgca cgacctcgaa gtgcatcatc
      661 atgcctgcgg cgtggtgctc gaggatgtgg cagtgcgcca tccaggcacc gggccgatcg
      721 tccggcatcc aggcgatctt gacccgcccc ttcggcggca cgttcacggt gtcctcccag
      781 gaccgatagg ccggcgcttc gccgttcacc gccagcacct ggaagaagaa gccatgcagg
      841 tggaagggat gatcgagcgg cgtctcgttg atgacatccc acacctggag ctcgccgacg
      901 cgcacgggct ggccgtgatg atgcggctcg tcgttcacca tgaaatcgac gccgcgccgg
      961 aggctcatgc ggccgcccag gcggacctcg cgcgtcacag gtgcatcggg tggggcgatt
     1021 ggatcaatcg tccgcagctt ctctgaaata cgcgcaacgg aacgtcgcga taaaccgacc
     1081 cgcacggtgc caaaggtgac cgccgttccc ttcaccccga ggccgcggtt gtactccaac
     1141 gactgaatgt cgagacgctg gctctcctcg aacggcccga cgaggaggtc gacgcgatcg
     1201 gcaggcgcga gcagcacctc ggtcgcgtcc acgggtgcct cgatgaggcc gccgtccgtg
     1261 ccaatgatcc ggaagggagc accgccgagc gagaaccgga cgtagcgcgc gctcgcggta
     1321 ttcacaagac gccagcgctc tacctgaccg gcagccatct cgagctcagg ccgcacgcgc
     1381 ccattgagca acgggacccg gccttcgcgg ccgttgtgcc gctcgagcca tccgccggga
     1441 ccggcaagtc ggccgcgcca gtcgaggcgc acgtcgtcaa acacgagcac gcgttcggca
     1501 tcgacctgcg gttcgtcggg gccgtgcacg acgagcacgc catatagccc tcgctcgagt
     1561 tgcacggttt cgttggcgtg cgggtgatac cagaacgtcc ccgcgtcggg cagggcgaag
     1621 cggtactcga acgcctcgcc cggcgcgacc gcctgctgga cactttctgt gccgtccatc
     1681 gcgggctgga ggcgcagacc gtgccaatgg atcgtcgttg gctcgtccaa acggttcgtg
     1741 agccggacga ccagcgtgtc gccctggcgc gcttcgatcg ttggccctgg cgtgatgccg
     1801 ttgtaagacc acccgtccag cgctttgtga ccgccgccga tatcccaccc ggcggctctt
     1861 gcctcgagcg cgacgaccac ttcgcggccc ggattcgtga ccaggggggt gtagtcggct
     1921 gcggcgttct tgtctgactc gtgcgtgcag tgattcgtca tggccgtcgt gtctcctctc
     1981 aagttcgaat gaaaggatag cgagcaggcc cacgctcagg attgaacgag gcggccgaaa
     2041 cgcttgaacg gggctgtcga agtacttgaa taaactggct gccccagttg cccgctcagg
     2101 tgattcacga ggaaccgggc atcgttgccg acgccccaca ggagcgcgga gccgcgcgtc
     2161 cattgccagc ttctcccgag taggtagagc ccccgagcgg gcgtgatgcc cctatggtgg
     2221 atgaagccgc ggtgctggtc cttcgcctca ggaatgtcta cccacccggt ctcttcgtgg
     2281 tagccggccg tccagacgac ggcatcgacg tgcagttcct ggccat
//
LOCUS       DATLFO010000270         5846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_26643, whole genome shotgun sequence.
ACCESSION   DATLFO010000270 DATLFO010000000
VERSION     DATLFO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26643"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2960
                     /locus_tag="VM841_11085"
     CDS             <1..2960
                     /locus_tag="VM841_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVL90766.1"
                     /translation="LCLIAMAVAGGGARAAGSFEGIFQYVHGDSPDFASPGQHAFMLT
                     TSDGSTHDLAPLPGAFDVGPDDLQGETVRVQGYRDPKGTIHASDVSPTDQGFSPADES
                     PITGSTPWAVLLCKYSNISAETNPLSYYTALMDNNFPRLDHYWRAQSYNAINLIGTQA
                     HGWFTLPQTESYYTNPANSNSTNLTNLAQDCTNAANPTVNFAPMTGIIVMVNGAVTGG
                     AGGWGGGRHLTLDGVTKVWPFAWMPSSGHWSGVVAHEMGHGYGLPHSSAQPYPSGGDY
                     QSDWDVMSSGFWNVGATYAPYSSAGTGTIAYHKDKLGWVSATRKFTPASGSATTITLW
                     PLGNAAPPGSQHLMAQIPIGATSRFYTVEARRKLPAPNYDGGVPADGVVIHEVDSTRS
                     ADALVVDLDGVYNQFPDPGEIWLPGETFVDSANGVSVEILTSNADGSFDVRIGLNVAA
                     VIAQHTGTDTTVTEDGATDTYTVRLNSQPTANVTVTAVHSGGQVSLSGPLTFTSANWA
                     TPQSVTVTAVDDTTAETSPHTALVTHDATSPDPLYNGTGLAPLTVNILDNEPVASVSP
                     ATIAFGNVEVGTQSPSQTITVTNAGQGTLAIGAVAITGTNAGDFQKTSDTCSGSSRAA
                     GQSCTIGVALTPSTTGLRSASVTIPHNAGGSSTVSLGGNGVQAAASLTPTSMSFGSAT
                     VGQQTGSQTATLTNTGTATLTLSTSFAGINPSDFLRVGGTCGSSLGAGGSCTILVAFK
                     PTAPGSRSASLLVTSNAPGSPHAVALSGTGVAAPTLSLNRTTVAFGNVEQGFTSPPQS
                     IIVTNTGGATLNIGTITISGAFASAFIKSSDTCSNAAVAPSTSCTIGVSFRPAALGGH
                     NATLTIPSNAAGSPHTVALSGLGVDTIKPLSAFTTTPGSIKVAGLDRVEGGTTDAGSG
                     VSRVMVYFTDLLGRTTQTLASLSCNAAKTSCTWTAPIALLLLPGIYSVNARATDVAGN
                     VETPGPTISIMVM"
     gene            3041..3478
                     /locus_tag="VM841_11090"
     CDS             3041..3478
                     /locus_tag="VM841_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HVL90767.1"
                     /translation="MAEPSSVETIRAAVDAILPGDGTLPSGGSLGIERHVVDLADGYL
                     TGFADMIAALLNAYATERGATAFADLDATGRIEVLRAMASEDVQDLRDAADAVFVFAY
                     GGMYSEWAGYDRAAGTLQPPQAWQRTGYHGPSDGHPSYREAAP"
     gene            3475..5073
                     /locus_tag="VM841_11095"
     CDS             3475..5073
                     /locus_tag="VM841_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVL90768.1"
                     /translation="MTDVLIVGAGGGGPVVAKELAERGLTVRMLEAGPWLDPSKDFSR
                     LEDDMNGIVHGRLRWGPSDRTRAPWRRRRDGAGLILQAAGVGGTTLHYNAIAPRAYPQ
                     SFASDWPFGYDEMIPWYERVEAFLPVVQVSDLATKDQVFADGCEAAGLKRSDERDCDT
                     AVWRPTFNAILPIATMKPGAPLRYPEVDGCTMCGHCLQGCPNPVGAPLERKAKRATNV
                     SYVPAAVATGNCTVVPDAFATAVLFERDQDGRARARGVRWRDTITGEIFEEDVSVVVL
                     AGGSVESPRLWLNSGLPNSNDAVGRYFTMHLQDLVTGFFDREIHPDVGQVTMARADFP
                     GIGTFWAQGYGPQAFAAALAGIGRGFWDDPVAAPFDLKGRFHGKEAMRLIAEYHRSLT
                     IVISTDDEAVSSNRVTLDPDWPADENGRVPLVTYHPTAESRRRQDWLAAKAAEVLRAA
                     GATRVHRTDPGVFITHLMSTMRIGSDPATSVFDPSGESHEVERLFIGDCAALPNSLGG
                     PNPTLTAQALAARTASRIADRYFS"
     gene            5070..5570
                     /locus_tag="VM841_11100"
     CDS             5070..5570
                     /locus_tag="VM841_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90769.1"
                     /translation="MKEVVIRPLRDGEIPAVTALLHRAYRRLAEQGLHFVAAHQDDDT
                     TRKRIRGGTCLVAEQNAVIVGTITYLPPGEKGGCEWYDRPDVAEFEQYAVEPSLQGSG
                     IGQRLLDEAERMARADGAAEMACDTAAPATGLIAMYLARGYRRVQPVDWRPFTNYESV
                     VLSKRL"
     gene            complement(5632..>5846)
                     /locus_tag="VM841_11105"
     CDS             complement(5632..>5846)
                     /locus_tag="VM841_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90770.1"
                     /translation="RQARGGIVDRLRTAAGKGVTLGELSSAMHHHERSLDWLVEILTQ
                     LESEGLAELSPAARRGSPRGTVRLPR"
BASE COUNT          964 a         2059 c         1942 g          881 t
ORIGIN      
        1 ttctgtgctt gatcgcaatg gccgtcgccg gcggcggtgc ccgcgcggcg ggctccttcg
       61 aaggaatctt ccagtacgtc cacggggaca gcccggattt cgcctcgccc ggccagcacg
      121 ccttcatgct caccacatcc gacggctcga cgcacgatct cgcgccgctg cccggtgcat
      181 tcgacgttgg ccccgacgac ttgcagggtg agaccgtccg cgtccagggc tatcgcgatc
      241 cgaagggcac catccacgcg tccgacgtct cgcccacgga tcagggattc tccccggccg
      301 atgagtcgcc gatcaccggc tcgacgcctt gggcggtgtt gctttgcaaa tactcgaaca
      361 tttctgccga gacgaacccg ctctcgtatt acacggcctt gatggacaac aacttcccgc
      421 ggttggacca ctactggcgc gcgcagtcct acaacgcgat caatctgatc ggaacgcagg
      481 cgcacggatg gttcaccctg ccgcaaacgg agagctacta caccaacccc gcgaactcca
      541 acagcaccaa ccttacgaac ctcgcgcagg actgcaccaa cgcggccaac ccgaccgtga
      601 acttcgcgcc gatgaccggc atcatcgtca tggtcaacgg cgcggtgacc ggaggggccg
      661 gcggctgggg cggtggacgc cacctgacgc tggacggagt caccaaagtc tggccgttcg
      721 cctggatgcc ctccagcgga cactggtcgg gcgtggtcgc acacgagatg ggtcacggct
      781 acgggttgcc gcactcgtcg gcccagccct atccctcagg aggcgactac cagtccgact
      841 gggacgtcat gtccagcggc ttctggaatg tcggagcgac ctacgctccg tacagctcgg
      901 ccggtaccgg cacgatcgcc tatcacaagg acaagctcgg ctgggtctcg gcgacgcgca
      961 agttcacgcc ggcatcgggt tcggcgacca cgatcacgtt gtggccgctg ggcaacgcgg
     1021 cgccgccggg ttcgcagcat ctgatggcac agatcccgat cggcgccacg tcgcgcttct
     1081 acacggtcga ggcgcggcgc aagcttcccg cccccaacta cgacggcggc gtgccagccg
     1141 acggcgtcgt catccatgag gtcgattcga cgcgctcggc ggacgccctg gtggtagacc
     1201 tcgacggcgt ctacaaccag tttcccgacc cgggggagat ctggctgccg ggcgagacgt
     1261 tcgtggactc cgccaacggc gtcagcgtcg agatcttgac ctccaacgca gacggctcgt
     1321 tcgatgtgcg catcggcttg aacgtggcgg ccgtcatcgc ccagcacacc ggaaccgaca
     1381 cgaccgtgac cgaggacggc gcgacggaca cctacacggt caggctgaac tcgcagccaa
     1441 ccgcgaacgt gaccgtgacg gcggtgcatt cggggggtca ggtgtccctg tccgggccgc
     1501 tgacgttcac cagcgcgaac tgggcgacgc cgcaatcggt gacggtcacc gcggtggacg
     1561 acaccacagc ggaaacctcg ccgcacacgg cactggtcac ccacgacgcc acgagccccg
     1621 atccgctcta caacggaacc ggcctggcgc cgctgacggt caacatcttg gacaacgagc
     1681 cggtcgcctc ggtcagcccc gcgacgatcg cgttcggcaa cgtcgaggtc ggcacccaga
     1741 gcccttcgca gacgatcacg gtgaccaacg ccggccaagg cacgctcgcc atcggagcgg
     1801 tcgcgatcac cggcaccaat gccggcgact tccagaagac gtcggacacg tgctcgggct
     1861 catcgcgagc cgcagggcaa tcgtgcacga tcggcgtcgc gctcacaccg tccaccactg
     1921 gcctgcgaag cgcgtcggtc acgatcccgc acaacgccgg gggctcatcg acggtgtcgc
     1981 tgggcggcaa cggcgtgcag gccgccgcgt cgctcacgcc gacgtcgatg tcgttcggct
     2041 ccgccacggt cggccagcag accggctcgc agaccgcgac actgaccaac accggcaccg
     2101 cgaccttgac gctgtccacc tcgttcgccg ggatcaaccc gagcgacttc ctgcgcgtcg
     2161 gcggcacctg cggctcctcg ctcggcgccg gaggctcctg cacgatcctg gtcgcgttca
     2221 agccgaccgc gccgggctcg cgctcggcct cgctgctggt gacatccaac gcgcccggct
     2281 cgccacacgc ggtcgcgctc agcggcaccg gcgtcgccgc gccgacgctg agcttgaacc
     2341 gcaccacggt ggcgttcggc aacgtcgaac agggtttcac ctcgccgccg cagagcatca
     2401 tcgtcaccaa caccggaggc gccacgctga acatcggcac gatcacgatc agcggggcct
     2461 tcgcgtcggc gttcatcaag tcgagcgaca cctgctcgaa cgccgcggtc gcgcccagca
     2521 catcgtgcac catcggtgtc tcgttccggc ctgcggcgct cggtggccac aatgcaacgt
     2581 tgacgatccc atcgaacgcg gccggcagcc cgcacacggt cgcgctgtcc ggcctgggag
     2641 tggacacgat caaacccctg tcggccttta cgaccacgcc ggggtcgatc aaggttgccg
     2701 gcctggaccg ggtcgagggt gggacgaccg acgctggatc gggcgtcagc cgtgtgatgg
     2761 tctacttcac cgatctgctg ggccgcacca cgcagacgct cgcctcgctg tcgtgcaacg
     2821 cggccaagac cagttgcacg tggactgcgc cgatcgcgct gctgctgctg cccggcatct
     2881 actcggtcaa cgcacgggcc accgatgtgg ccggcaacgt cgagactccc ggcccgacga
     2941 tctcgatcat ggtgatgtag cggaccaccg cagcatccaa cggccgccgg acccgaccgg
     3001 gtgcggcggc cgtttcgcgt ccggaccgta gaatgcggcc gtggccgagc cgtccagcgt
     3061 cgaaaccatc cgcgccgctg tcgacgcgat ccttccgggt gacggcaccc tgccgtcggg
     3121 ggggtcgctc ggcatcgagc ggcacgtggt cgatctggcc gacggctacc tgaccggctt
     3181 cgccgacatg atcgcggcgt tgctcaacgc ctatgcgacc gaacgagggg ccaccgcgtt
     3241 cgcggacctg gacgcgaccg ggcgtatcga ggtcctgcgc gcgatggcat ccgaagacgt
     3301 gcaggatctg cgcgacgccg cggatgcggt cttcgtgttc gcctacggcg gcatgtactc
     3361 ggagtgggcc ggctacgacc gcgcggccgg cacgctacaa ccgccgcaag cgtggcaacg
     3421 aaccggctat cacggcccga gcgacggcca tccgagctat cgggaggcgg ccccgtgacc
     3481 gacgtgctca tagtcggcgc aggcggcggt ggtccggtgg tcgccaagga gctggccgag
     3541 cgcggcctga ccgtgcggat gctcgaggcc gggccgtggc tggatccttc gaaagacttc
     3601 tcgcgtctgg aagacgacat gaacggaatc gtgcacgggc gcctgcgctg gggaccgtcg
     3661 gaccgcacgc gtgcaccgtg gaggcgccgc cgcgacgggg cgggcctgat cctgcaagcc
     3721 gccggggtcg gcggcaccac gctgcactac aacgccatcg cgccgcgcgc ctatccgcag
     3781 tcgttcgcgt cggattggcc gttcggatac gacgagatga tcccgtggta cgaacgcgtg
     3841 gaagcgttct tgccggtcgt gcaggtctcg gatctggcga ccaaagatca ggttttcgcc
     3901 gacggctgcg aggccgccgg gctgaagcgc agcgacgaac gcgactgcga tacggcggtg
     3961 tggcgaccca ccttcaacgc catcctgccg atcgcgacga tgaagccggg cgcgccgctg
     4021 cgctatccgg aggtggacgg ctgcaccatg tgcggccact gcctgcaggg ctgcccgaat
     4081 ccggtggggg cgccactcga acgcaaggcc aaacgcgcga ccaacgtctc ctacgtgccg
     4141 gcagccgtcg cgacgggcaa ctgcacggtc gtgcccgacg cgttcgccac cgcggtcctg
     4201 ttcgagcgcg accaggacgg gcgcgcgcga gcgcgcggcg tgcggtggcg cgacacgatc
     4261 accggcgaaa tcttcgagga ggacgtctca gtggtcgtgc tggcgggcgg ctccgtggaa
     4321 tcgccgcggc tgtggctcaa cagcgggctt ccgaactcca acgacgccgt cggccggtac
     4381 ttcacgatgc acctgcagga tctggtcacc ggcttcttcg atcgcgagat ccacccggat
     4441 gtcggacagg tgacgatggc gcgggcggac ttcccgggca tcgggacgtt ctgggcgcag
     4501 ggctatgggc cgcaagcatt cgcggccgcg ctggccggca tcggtcgcgg cttctgggac
     4561 gacccggtcg cggctccttt cgatctgaaa ggacgcttcc acggcaaaga ggcgatgcgg
     4621 ctgatcgcgg aataccaccg gtcgctcaca atcgtgatct caactgacga cgaggcggtc
     4681 tcgtcgaacc gggtgacgct cgatccggac tggccggccg acgaaaacgg ccgcgtgccg
     4741 ctggtcacgt accacccgac cgcggagtcg cggcgccgtc aggattggct ggccgcgaaa
     4801 gccgccgagg tgctgcgcgc cgccggcgcg acgcgtgtgc accgaaccga cccgggcgtg
     4861 ttcatcacgc atctgatgag cacgatgcgc atcggctcgg atccggcgac gagcgttttc
     4921 gatccgtccg gcgagtcgca cgaagtcgag cgcctgttca tcggcgactg cgcggcgctg
     4981 cccaacagcc tcggcggacc gaacccgacg ctgaccgcgc aggcgctggc cgcgcgcacg
     5041 gcgtcacgga tcgcggatcg ctacttctcg tgaaagaggt cgtcatccgt ccactgcgcg
     5101 acggcgagat cccggcggtt accgcgctgc tgcaccgcgc ctaccggcgg ttggccgagc
     5161 aaggattgca cttcgtggcg gcgcatcagg acgacgacac gacacgcaag cgcatccgag
     5221 gcgggacctg cctggtcgca gagcaaaacg ctgtcatcgt cggcacgatc acctatcttc
     5281 cgcccggcga gaagggcggc tgcgaatggt acgaccggcc cgatgtcgcc gaattcgaac
     5341 agtacgcggt cgagccctcg ctccaaggaa gcggcatcgg acagcgcttg ttggacgagg
     5401 ccgagcggat ggcgcgcgcc gacggcgccg ccgagatggc ctgcgacacg gccgctccgg
     5461 cgaccggcct gatcgcgatg tacctggccc ggggctaccg ccgggtgcag ccggtggact
     5521 ggcgtccctt cacgaactac gaaagcgtcg tcctctccaa gcgcctgtag ccggcgcttc
     5581 cttgtgcggt atgcatccac cgcatgttca gggtgtacaa ggacgcggtc gttagcgcgg
     5641 caggcggacg gttccacgcg gcgaaccgcg gcgtgcggcc ggactcagtt ccgccagccc
     5701 ttctgattcc agctgcgtga ggatctcgac cagccagtcg agcgaccgtt cgtggtggtg
     5761 catcgccgac gacagctcgc ccagcgtcac gcccttgcct gcggccgtcc gcagccggtc
     5821 gacgatgccg ccgcgcgcct gccggc
//
LOCUS       DATLFO010000271         2219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88740, whole genome shotgun sequence.
ACCESSION   DATLFO010000271 DATLFO010000000
VERSION     DATLFO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88740"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="aroC"
                     /locus_tag="VM841_11110"
     CDS             <1..1015
                     /gene="aroC"
                     /locus_tag="VM841_11110"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HVL90771.1"
                     /translation="MKFEEDRLEILGGVRHGRSIGDPIAMVIRNTEWPKWEQIMAPEE
                     EDAVLEDPALTRPRPGHADLAGLLKYDAPDARNILERASARETAARSAIGAVCKSFLG
                     GLGIQIVSHVTAIGSVQAAETEPPRSEDLERIDATALRCLDAAAEQEMIALIDAMRKK
                     GDTLGGVFEVVVWGLPPGIGSHVHWDRRLDASLAQAMMSIQAIKGVEIGRGFDVAAMP
                     GSQAHDEIEYEGGEYLRRTTRAGGTEGGISIGGPVIVRAAMKPLSSLARPLKTVDVNT
                     KGEAVAITQRSDVCAVPAAAVVGESMAAYAVSAAVLDKFGGDSLGETKRNLDAYVAAI
                     KAR"
     gene            1018..1533
                     /locus_tag="VM841_11115"
     CDS             1018..1533
                     /locus_tag="VM841_11115"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HVL90772.1"
                     /translation="MTSRNVILIGFSGSGKSTVASLLAARLGYERLDTDAMVAASAGR
                     SVHEIFQRDGETAFRAMESRAVEQAAAGNARVIACGGGAILALRNYTVLRDAGTIVYL
                     RTSPGEILARLGDAPDRPLLRGKAGRAVPALLAERSPAYQSAADLIVDTDGKSAGEVA
                     DEIHRLLKERP"
     gene            1530..>2219
                     /locus_tag="VM841_11120"
     CDS             1530..>2219
                     /locus_tag="VM841_11120"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HVL90773.1"
                     /translation="MTVRTLEVGGWAGPYRIHVGANTLSAIGELVTLVHERAALIHDE
                     NLPAAIVDRAAGAIEAGDVELIRIPVPSGEAAKSFAVAERVARAAAAAGLHRGDLIAG
                     LGGGATTDLSGFVAATYHRGVDHLALPTTLLGMVDASIGGKTAVNIPEGKNLIGVFHS
                     PVAVVADVETLLTLPDGEMQSGLAEVVKHGLIGDPGLLGDLRAHATPLTDRDPETLTD
                     LVARAAAVKVAV"
BASE COUNT          343 a          787 c          775 g          314 t
ORIGIN      
        1 catgaagttc gaggaggatc gccttgagat cctcggcggg gtgcgtcacg gccgctcgat
       61 cggggatccg atcgcgatgg tcatccgcaa caccgagtgg cccaagtggg agcagatcat
      121 ggctcccgag gaagaggacg ccgtcctcga agatcccgcg ctgacccgtc cgcgtcccgg
      181 gcacgccgat ctggcggggc tgctgaaata cgacgcgccg gacgcgcgca acatcctcga
      241 gcgcgcgagc gcccgggaga ccgccgcacg cagcgcgatc ggcgcagttt gcaagtcttt
      301 cctcggcggc ctcgggatcc agatcgtgtc ccacgtcacc gcgatcggct ccgtacaggc
      361 ggccgagacc gagccgccac gttccgagga tctcgagcgc atcgacgcga ccgcgctgcg
      421 ctgcctcgac gcggcggcgg agcaggagat gatcgcgctg atcgacgcga tgcgcaagaa
      481 gggcgatacg ctcggcggcg tcttcgaggt cgtcgtgtgg ggccttccgc ccggcatcgg
      541 gagccacgtg cactgggacc gccggctcga cgcgagcctc gcgcaggcga tgatgagcat
      601 ccaggcaatc aagggcgtgg agatcggccg cggcttcgac gtcgccgcga tgcccggctc
      661 gcaggcccac gacgagatcg aatacgaagg cggcgagtac ctgcgacgga ccacgcgcgc
      721 gggcggcacc gagggcggca tcagcatcgg cggtcccgtt atcgttcggg cggcgatgaa
      781 gccgctgtcg tcgctggcgc ggccgctcaa gaccgtggac gtcaacacca agggcgaggc
      841 cgtcgcgatc acgcagcgct ccgacgtgtg cgcggtgccc gccgccgcgg tcgtgggcga
      901 gtcgatggcc gcctacgcgg tctcggccgc cgtgctcgac aaattcgggg gcgactcgct
      961 cggcgagacc aaaagaaacc tcgacgccta cgtcgcagcg atcaaggcac gctgaccgtg
     1021 accagccgca acgtgatcct catcggcttt tccggctcgg gaaagagcac ggtcgcgtcg
     1081 ctgctcgccg cgcggctcgg atacgagcgg ctcgacaccg acgcgatggt cgcggcctcg
     1141 gccggccgat ccgttcacga gatcttccaa cgcgacggtg aaaccgcctt ccgcgcgatg
     1201 gagtcccgag cggtcgagca ggccgcggcc ggaaacgccc gggtgatcgc gtgcggcggc
     1261 ggcgcgatcc tcgcgcttcg caactacacc gtcctgcgcg atgccggaac catcgtctac
     1321 cttcgcacct cgcccgggga gatcctcgct cgcctcggtg acgcacccga ccgtccgctg
     1381 ctgcgcggca aggccggccg cgccgtgccg gcgttgctcg ccgaacgttc gcccgcctat
     1441 caatcggccg cggacctgat cgtggatacc gacggcaaga gcgccggcga ggtcgcggac
     1501 gagatccacc gcctgctgaa ggagcgtcca tgacggttcg cacccttgag gtcggcggct
     1561 gggccggtcc gtaccgcatc cacgtcggcg cgaacaccct gtcggcgatc ggcgagctgg
     1621 tgacgctcgt ccacgagcgc gcggctttga tccacgacga gaacctgccc gcggcgatcg
     1681 tggaccgggc cgccggagcg atcgaagcgg gagacgtcga gctgatccga atcccggttc
     1741 cctccggcga ggccgcgaag tccttcgcgg tcgccgagcg cgtcgcgcgc gccgccgccg
     1801 ccgccgggct gcatcgcggc gacctgatcg ccgggctcgg cggcggcgcg accacagacc
     1861 tgtcgggctt cgtcgccgcg acttatcacc gcggcgtgga tcacctcgcg ctgccgacga
     1921 cgctgctcgg catggtggat gcgtccattg gcggaaagac cgccgtgaac atcccggaag
     1981 gcaagaacct catcggcgtg tttcactcgc cggtcgcggt cgtcgccgac gttgagacgc
     2041 tcctgacgct gcccgacggc gagatgcagt ccggtctggc cgaggtggtc aagcacgggc
     2101 tgatcggcga ccccggcctc ctcggcgatc tgcgcgcgca cgcgacgccc ctcaccgatc
     2161 gcgatcccga gacgctgacc gatctggtcg cgcgcgccgc cgccgtcaag gtcgcggtc
//
LOCUS       DATLFO010000272         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_65386, whole genome shotgun sequence.
ACCESSION   DATLFO010000272 DATLFO010000000
VERSION     DATLFO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65386"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VM841_11125"
     CDS             <1..988
                     /locus_tag="VM841_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL90774.1"
                     /translation="ASAGVGRAAARLFATRGYDVGLIARDRSRLEATAAEVRAAGRRA
                     AIAECDLADDKAVMTAAADLASALGPPDVWVNNAMTSVFAEFTDIESDEFRRVTEVTY
                     LGAVYGTRAALDRMLPRDRGVIVQVGSALAHRAIPLQSAYCGAKHALDGFTESLRCEL
                     LHRRSNVKVTIVQMPALNTPQFDWVLSRLPNRSQPVPPIYQPEVAARAIVHAAEHPRR
                     EMWVGATTVATILAQKVMPGLLDRYLARTGYRGQQTVVPADPSRPSNLWRPVPGHYAA
                     RGRFSDRALSVSVQSMLNRHRVAAAAVAAAGTALGRVALTAMRRNGASPASE"
     gene            1201..1950
                     /locus_tag="VM841_11130"
     CDS             1201..1950
                     /locus_tag="VM841_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263013111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1408..1410,aa:Sec)
                     /transl_table=11
                     /product="deiodinase-like protein"
                     /protein_id="HVL90775.1"
                     /translation="MAGYWYEHFDDYVASGAEERDFDAFPGSEPHATGRAPDGTLTRL
                     SDGEPVQLSDLWRSQDVVLEFGSFTUPYCTAEGPLLDDLADEFTDIAWYFVYTAEAHP
                     GEHVPQHRTFDDKLAAARRLRDEAPIRREILLDDMNGTVHSAYGLMPNMTYVIGRRGI
                     VLSKSMWTAARSVREYLNRYREAMQMGAVLMYSEHAEFRYPDRELFMRLLERAGPSAV
                     TEFKEGMEFIAERDRTRARQRRQRQQQRPPH"
     gene            2125..2192
                     /locus_tag="VM841_11135"
     tRNA            2125..2192
                     /locus_tag="VM841_11135"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(2391..2740)
                     /gene="ssrA"
                     /locus_tag="VM841_11140"
     tmRNA           complement(2391..2740)
                     /gene="ssrA"
                     /locus_tag="VM841_11140"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2959..>3399
                     /locus_tag="VM841_11145"
     CDS             2959..>3399
                     /locus_tag="VM841_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL90776.1"
                     /translation="MTGGRLAELYREHAAGARRVAYLLTGDEQTAEDLVQDAFVRLAG
                     RFLAFSSAAAAGSYIRRTVASLTVSRFRRIASEDRRDTRHAAMATTVHHDPDVTERDR
                     MWRALQTLPERQRAAIVLRYYTDLSDDDIADTLRCRPGTVKSLLS"
BASE COUNT          540 a         1184 c         1121 g          554 t
ORIGIN      
        1 cgcgagcgcc ggcgtcggcc gggccgccgc gaggctgttc gcgacccgcg gctacgacgt
       61 cggcctgatc gcacgcgacc gctcgcgcct cgaggccacc gccgccgagg ttcgcgccgc
      121 cggccgccgt gcggcgatcg ccgaatgcga cctggccgac gacaaggccg tcatgacggc
      181 ggccgcggat ctggcgtcgg cgctgggccc cccggacgtg tgggttaaca acgcgatgac
      241 gtcggtgttc gccgaattca ccgacatcga aagcgacgag ttccggcgcg tgaccgaggt
      301 cacgtacctg ggcgcggtct acggcacgcg cgccgcgttg gaccggatgc tgccgcgcga
      361 ccgcggcgtg atcgtgcagg tgggatccgc gctcgcgcac cgcgcgatcc cgctccagtc
      421 cgcgtactgc ggcgccaagc acgccctcga cggtttcacc gaatcgcttc gctgcgagct
      481 gctgcatcgc cggtcgaacg tgaaggtgac gatcgtccag atgcccgcgc tgaacacgcc
      541 acagttcgac tgggtgctgt cgcgcctgcc gaaccggtcg caaccggtgc cccccatcta
      601 tcagcccgag gtcgcggcgc gagccatcgt gcacgccgcc gagcatcccc gccgggagat
      661 gtgggtggga gccacgacgg tcgcgacgat cctcgcccag aaggtcatgc ccggcctgct
      721 cgaccggtac ctcgcccgca ccggctatcg aggccagcaa accgtcgtcc cggccgatcc
      781 gtcgcgcccg tcgaacctat ggcgtccggt tcccggccac tacgccgcgc gcggacgctt
      841 cagcgaccgg gcgctgtccg tcagcgtcca gtcgatgctc aaccgccacc gcgtcgcagc
      901 ggcggccgtc gctgcggccg gcacggccct gggccgggtc gcgctcacgg cgatgcgccg
      961 taacggcgct tcccctgcga gcgagtgatg gtgccgcacg ccgcggagat ttgcgcctcg
     1021 tccggggact tgggcgcgat gatcgcgaac tcgcctcgag cagcgcgttc agcccgacct
     1081 cgacttcggt cgtggaccgg cccttctcga cgtcggcgag cggatgctcg ggtgccggat
     1141 gcacgagacg gtcggcacgc agctgcaggg tctttaaccg aacccggtag gctgtcgccc
     1201 atggccggct actggtacga gcacttcgac gactacgtcg cctccggcgc cgaggaacgc
     1261 gacttcgacg ccttcccggg ctcggagccg cacgcgaccg gccgcgcacc cgacgggacg
     1321 ctgacgcgtc tgtccgacgg cgagccggtg cagctgtcgg atctgtggcg ctcgcaggac
     1381 gtggtgctcg agttcggctc gttcacctga ccgtactgca cggcagaggg tcctctgctc
     1441 gacgatctgg cggacgagtt caccgacatc gcttggtatt tcgtctatac cgccgaggca
     1501 cacccgggcg agcacgtgcc gcagcaccgg acgttcgacg acaagctcgc cgccgcgcgc
     1561 cggctgcgtg acgaagcgcc gatccggcgt gagatcctgc tggatgacat gaacgggacc
     1621 gtgcacagcg cctacgggct gatgccgaac atgacgtatg tgatcggccg ccgcggcatc
     1681 gtgctgtcca agtcgatgtg gaccgcggcc cggtccgtgc gggagtattt gaaccgctac
     1741 cgcgaagcga tgcagatggg cgccgtgttg atgtacagcg aacacgccga gttccgctat
     1801 cccgaccgcg agctgttcat gaggctgctc gaacgagccg gcccgagcgc cgtcaccgag
     1861 ttcaaagaag gcatggagtt catcgccgaa cgcgaccgaa cccgcgcccg gcagcgccgc
     1921 cagcggcaac aacaacggcc gccgcactag cgacggccgc acaatcgtgg agctaacggg
     1981 ttgctattcg aaccttcgcg tcgtgctgga ggactgggta tttctgctcg ctcttcccgc
     2041 tcttcccgaa gatgaaatcg cttgcacggt ccgtctatcg tccacggagc cgaccgatcg
     2101 gctgcaaggg aggctgacga tggggccggc gcgatggttc ggacacgggg ctcgtcatcc
     2161 cggtgttggg agttcgattc tccttgccgg ctctcctccg cagcgagaaa gccccgaccg
     2221 aagtcggggc tcagacacga ggctcgtctc cagcaagtta gcgcccgcgg gccgcctccg
     2281 caagggaccc agaaagggcc tgcggtacgc gaagttcgaa tccccttcgc ggtcttacat
     2341 attccggcgc ctttcaccgg tgttgacctg cgtcgatccg gagcgaaact aagtggaggt
     2401 ggcgggaatc gaacccgcgt ccttcgacct cggacccaag gcatctccga gcgcagccgg
     2461 cgatttggtt ctcggtcctc ggacgcgcac cggctcgctt cctcggaccc agcccggttg
     2521 atgtccgctc ccctacccgg acgctcgggg agcgtgagcc cgctgaatga cgttcctgcc
     2581 cgggacgcgg gcgcgcccgg aggaacggct gcctaatcta gttaggcggc gagtgcgtga
     2641 ttgtccgcac ttatattttt tccggtttga tgacgaggcc ccggagaccc cggctcgctt
     2701 ccttggattc gaccgatcga agtcgaaacc atgcaccccc gatgctgttg tcaacctcct
     2761 ccagtgtacc ggccgccgcc gtcgtccgcc ccaccgattt cgtttttggt cggtccgaac
     2821 acgtaatgcg tgttccagtt gaccaagaat gagcgcctga cgggttcggc tgcaggaaaa
     2881 tgtcctcagg ggtgcaacct cgagcgcccc ggcaagcgtt caaagggcat ggaggggatc
     2941 gcggtcgata gtaagccgat gacgggcgga cgcctggccg agctgtaccg cgagcatgcg
     3001 gccggcgccc ggcgggtcgc ctatctactg accggcgacg agcagacggc ggaggatctg
     3061 gtccaggatg cattcgtgcg tctggccggg cgcttcctgg ccttctcttc ggccgcggcc
     3121 gccggctcct acatccggcg caccgtcgcg tcactgaccg tgtcacggtt ccgaaggatc
     3181 gcctccgaag accggcgcga cacacggcat gcggcaatgg caacgaccgt ccatcacgac
     3241 ccggacgtga ccgaacgcga ccgaatgtgg cgagcgctgc aaaccctgcc cgagcgacag
     3301 cgcgccgcga tcgtgctgcg ctactacacc gacctgagcg acgacgacat cgccgacacg
     3361 cttcggtgcc ggccaggcac cgtcaagtcc ctgctgtcg
//
LOCUS       DATLFO010000273         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_26785, whole genome shotgun sequence.
ACCESSION   DATLFO010000273 DATLFO010000000
VERSION     DATLFO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26785"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1129
                     /locus_tag="VM841_11150"
     CDS             152..1129
                     /locus_tag="VM841_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90777.1"
                     /translation="MLGPENGIIKAALEGWKDEVGKNLEDVFDADSKSSAIAKIDEVV
                     RTGVEKQLGDLRKMLHDPTDGPLGRMYGAITKAVKDQGETLGKEIAELAEALKIQAAK
                     AEVEKATSRKGFAYEDLLHAAVTNVVAANGDVAEKVAKTKGLAGTEKGDEIVTVNTDD
                     TLGTVVAYVIEAKDNGSYSVRTALKECETAMKNRGTKVGIIAFANAGQIAIDGDFHVF
                     GDKAIIVYDREDPDPRVLKIACAWGRAEARRKMAGTGPTIDLSRIESLVADARRALGR
                     GKTIKSCLTVGKKQMDSAGAELTALLDEVDTVLDDVCNELNKSGEKDAA"
     gene            complement(1352..1423)
                     /locus_tag="VM841_11155"
     tRNA            complement(1352..1423)
                     /locus_tag="VM841_11155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Val,seq:tac)
     gene            1587..>2733
                     /locus_tag="VM841_11160"
     CDS             1587..>2733
                     /locus_tag="VM841_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVL90778.1"
                     /translation="MGPLRGVTVLEFAGIGPGPFTAMMLADMGADVIRIDRPAAAGGS
                     RSVVTASARLDVLNRGRRSIALDLKHPEAANVALRLIDGADAIIEGFRPGVMERLGLG
                     PDVCLERNPRLVYGRMTGWGQDGPLAERAGHDIDYIAIAGALGSFRRTGEKPLPPVNL
                     VGDFGGGGLLLAFGIVAALFEASRSGQGQVVDAAMVDGAAILSTMIYGLRAQGVWNDT
                     PGTNILDTGAPFYEVYETADERHIAVGALEPQFYAEFIRLAGLEGIADPEGVRFDRSR
                     WPEMKQRYAEHFKTKTLAEWTEIFEGSDACVAPVLSMDEAVAHPHNDTRRTFVEAGGI
                     PQPAPAPRFSRTPPSIRRPPPAPGEQTDEVLAGRGFDPAEIARLKAAG"
BASE COUNT          520 a          875 c          930 g          408 t
ORIGIN      
        1 atgagcgtcg aagcgctcac caaagcacgc atgcacgtca tcgagagcga agccgaggag
       61 gtgaccgcgg aactccccgc cgcggtgtga atgagaccgg atgagacggt ggcttcatac
      121 accaccttga cggacgcggc ccgccgattc gttgctcggt cccgagaacg gcatcatcaa
      181 ggccgcgctt gaaggatgga aggacgaagt cggaaagaac ctcgaagatg tctttgacgc
      241 cgacagcaag agcagcgcca tcgccaagat cgacgaggtt gtgcgcaccg gcgtcgagaa
      301 gcagctcggc gaccttcgca agatgcttca cgacccgacc gacggaccgc tcgggcgcat
      361 gtacggcgca attaccaagg ccgtgaagga tcagggcgag acgcttggaa aggagatcgc
      421 cgaactcgcc gaggcgctga agattcaggc tgctaaggcc gaggtcgaga aggcgacgag
      481 ccgcaaggga ttcgcctacg aagaccttct ccacgcagcc gtgacaaatg tcgtcgcggc
      541 gaacggcgac gttgcggaga aggtcgcgaa gacgaagggc ttggccggca ccgaaaaggg
      601 tgacgagatc gtcacggtga acacggacga cacgctcggc accgtcgtgg cctacgtcat
      661 cgaggctaag gacaacggct cctacagcgt tcgtacggcg ctcaaggagt gcgagacggc
      721 gatgaagaac cgtggaacca aggtcggcat catcgccttc gcgaatgccg gtcagattgc
      781 gatcgacggc gacttccacg tcttcggcga caaggcaatc atcgtctacg acagggaaga
      841 tccggatccg cgcgtcctga agatcgcgtg cgcgtggggt cgggcagaag cacggcgcaa
      901 gatggcaggg acaggcccaa caatcgacct ctcccgtatc gagagtctcg tcgcggatgc
      961 gcgtcgcgct ctcggccgcg ggaagaccat caaatcgtgc ctcaccgtcg gtaagaagca
     1021 gatggactcc gcaggtgccg agctgaccgc cctcctcgat gaggtcgaca cggtgctcga
     1081 cgacgtgtgc aacgaattga acaagagcgg cgagaaggac gccgcataga atagacgatc
     1141 atgttaagtg cgttggcgcc cgagcaaagc ggctcgggcg cttcgcattt cattccgttc
     1201 caggaccccg gcaggacccg cgggacccgc gcaggacccg taattgcggc ctgcaagggt
     1261 ctctgtcgga ccctagcggg agaaaccgcg agtccatggg atagtggcct ctacctgcgt
     1321 agacggacgt tttcgcatca taaacaggga gtgggcgcgg agggtgtcga acccccgacc
     1381 ttctgcttgt aaggcagacg ctctaccgtt gagctacgcg cccggtggtc gcagtctacc
     1441 gccggccggt cgcggccccg cgggttctgc gtcgagacga tagcggagaa ccggcacgag
     1501 ccggcgagga tgctcggcgc tgcctgatca cacgcgaccg cataatgttg ggcccaacga
     1561 acacgctttc gtcgggagga aagctgatgg gtccgctgcg cggtgtgacg gtgctcgagt
     1621 tcgcgggaat cggtcccggt cctttcacgg ccatgatgct cgccgacatg ggagccgacg
     1681 tcattcgcat cgaccgtccg gccgccgcgg gcggctcgcg cagcgtcgtc accgcgagcg
     1741 cgcggctgga cgtgctcaac cgaggccgcc gctcgatcgc gctcgacctg aagcaccccg
     1801 aggcggcgaa cgtcgcgttg cggctgatcg acggcgccga cgcgatcatc gaagggttcc
     1861 gtcccggcgt catggaacgg ctcggcctcg gccccgacgt gtgcctcgag cgcaacccga
     1921 ggcttgtcta cggccgcatg accggctggg gccaggacgg gccgctcgcc gaacgcgcgg
     1981 gccacgacat cgactacatc gcgatcgcgg gcgcgttggg ctcattccgc cgcacgggcg
     2041 aaaagccgct gccgccggtg aatctggtcg gcgacttcgg cggcggcggg ctgttgctgg
     2101 cgttcggcat cgtcgccgcg ctgttcgagg cgtcgcgctc gggccaaggg caggtcgtcg
     2161 acgccgcgat ggtggacggc gccgcgatcc tgtccacgat gatctacggg ctgcgcgcgc
     2221 aaggcgtgtg gaacgacaca ccgggcacga acatcctcga caccggcgcg cccttctacg
     2281 aggtctatga gaccgccgac gagcggcaca tcgcggtcgg cgcgctcgag ccgcagttct
     2341 acgccgagtt catccggctc gccgggctgg aaggcatcgc cgatcccgaa ggcgtgcgct
     2401 tcgatcggtc gcgctggccc gagatgaagc aacgctacgc cgagcacttc aagaccaaga
     2461 cgctggcaga gtggaccgag atcttcgaag gctcggatgc gtgtgtcgcg ccggtgctgt
     2521 cgatggacga ggccgtcgcg catccgcaca acgacacgcg ccggacgttc gtcgaggccg
     2581 gcgggattcc ccagcccgcg ccggcgccgc gtttctcgcg cacaccgccc tcgatccgcc
     2641 ggccgcctcc cgcccccggc gaacagaccg acgaggtgct cgccggccgt ggcttcgatc
     2701 ccgcagagat cgcacggctc aaggctgcgg gcg
//
LOCUS       DATLFO010000274        22639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_36982, whole genome shotgun sequence.
ACCESSION   DATLFO010000274 DATLFO010000000
VERSION     DATLFO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36982"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VM841_11165"
     CDS             <1..196
                     /locus_tag="VM841_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL90779.1"
                     /translation="DAHHLVPGRGRQVTDLVLLCRRCHRRVHRFKWKVRIQADGAITF
                     TARGRTHTSHPRHRSPPALE"
     gene            complement(347..3136)
                     /locus_tag="VM841_11170"
     CDS             complement(347..3136)
                     /locus_tag="VM841_11170"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HVL90780.1"
                     /translation="MTKPVRIALASLMSGVLLISGAAPAFASPSITSFAASPLVFSPN
                     GDGTRDATVISASINAQGTPTWTLTITNASGAIVRTFSQSGASVAQSWNGLDEENNAL
                     PDGDYSVQLSATDANGTALATVTTSIDLTPPRISMISPRKGGNVAYTSLPILLQIIDA
                     PPPSAGVAPTTSGANTSTMTMKVVDLQNFAAFQTPGFVAESANTGDTTISIKSQPVTL
                     IPGREYEVQATVSDNAGNPTTMKGRFLVMGTSAVSRPNVSIPQQPATTRISYNTSLDL
                     YQWATPLVSAGSYTVQFASTKHDGIGATRITFNPQFAEVTYTTGGIVWSTNLRESSAT
                     FDTSFRINTSGVVTQTISNGSGRTQTLSALIPKNADAGSVRMRLNPTPATTAAFPTCS
                     DPSANSYACTPDPITTLSLHETERLAWERLMDVDPYDVPAEVAVLMGLPLTSNRCEGT
                     PPTLATALDHFGAAVGTDTSMRSGITLSSEVSAATARIVECFARYVPVIASVANASLA
                     EQQTAWLEASRDISWAIKSIPAGATWPAEAVSLDILDKFVVGGTGNDTYTNHAILIIE
                     PGGNDTYPNNAGGALGTNIMTALAAVVDLAGNDTYGRSTHSFRACDEQPGPSIGAGTW
                     NAVGILIDADGADSRTGYSCSQGMGKRGLGLLLDLGNGSDTYSSYFSATNQNLQGPNG
                     LMGVGTNGGIGILIDPAADQSPTTFDAKGMYNIGYGDGEGLGLVIGGAGNTTYRNVNT
                     DTNIYGYGWSIGVATSGSKAVVLDPGGTDSYRCTGTNKKDFCVAVAWGPDDRDTAASA
                     GTLGMVVDRGPGNDTYYGDGIGGMSHASGSGAAAILFDAGGVDSYNCASAWCFGASAY
                     DGEGIFIDVGASDVDGYTCATSPAMKVPAQVLPTMPTSGACKGRGNARVWIGTRSVGN
                     TADVFGFGLDQVHAP"
     gene            complement(3245..3775)
                     /locus_tag="VM841_11175"
     CDS             complement(3245..3775)
                     /locus_tag="VM841_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90781.1"
                     /translation="MDVRLRIACSAAASTLTAAMLVSCTAEPMPSACRAPSPGSITDL
                     AQEPDYSDGGSLKRWRTADGCPVRLDVVMTRTGPEHCGFQSVRDIVTTWPPGSVPDTP
                     GRGDVRIFLNDAERVIAGNTKKAERIDRLPDEAQFTRLVGPDAALWYVPTDDTAIYLV
                     SDDRIERWPAGSYGCA"
     gene            complement(3807..5504)
                     /locus_tag="VM841_11180"
     CDS             complement(3807..5504)
                     /locus_tag="VM841_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90782.1"
                     /translation="MRSIVPAQSDWSAVWQTTRRRPTGVLPGDGLWARASFIRSGDTS
                     LAIVSLDVLGLFYEDVVRIREQIRGALGAGTHVLVASTHTHSSIDTLGIYGPDQTTSG
                     INPAYQAFVLSQAAGAAIDAAAAAQDVTEVRFGSKVPGETVNEYDRSRHPGTFDDSVN
                     VIQLRGASGAIGTIVNWASHPELIDSKSTSDPMIPASMRGAVMSSDYAHTLRTTVEAA
                     GGGTTVYLNGPNGAVTGLAVAIVDPDTGTLFPKKSVKKAYHVGRRIGLTALDALAAPS
                     ALSVTAPTLAVRSEEMFLTVENAFILALKAAGVVDRPTYTAGVESPAGRDVKTEMLHA
                     RFGPAEFLTMPGELQPDLYTGVYQPMHERANPDVPPERAIEPQMTGDIHFVVGLGMDE
                     LGYFVSATDYVPPTLNPLYSNGVDRNGVSHYQETLSLGRDTARALSQQASRMLGHAPE
                     PDYFAYPGGFLTANGVAHYNDAGSPVHGIWGDTSDSGRYEAREDAQVFVPVPSAAGSA
                     YGYLDSQLRDAGKQLDARARGVWVDGDGDGGFDARRDPHLFFDTWMLGEGLLFKIPL"
     gene            5699..6562
                     /locus_tag="VM841_11185"
     CDS             5699..6562
                     /locus_tag="VM841_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HVL90783.1"
                     /translation="MSRTVGPVPDTSGLGAGDHVCWVYADDEDHRAGVTRYFADGIER
                     GERIVYVSDHYDEATVVAYLSAAGFAPKRMLEQGSLIVMPACESYPRDEHGYDLDATV
                     AQFQAFAQQAVADGYTGFRVAGESDFLLETVPQHGLLAYELTADLMVREAPMTGMCLY
                     DARHAPMERLGEVAAVHAAAVVASSLAADLPSFRLWASETETIGLAGEIDFATADTVR
                     QILMDGAVGRAGVLDLTTLGFADVAAIRAIARVAQAMAGQNDRPVIRGAASQVRFVLS
                     QFEVSSLVFDG"
     gene            6751..8484
                     /locus_tag="VM841_11190"
     CDS             6751..8484
                     /locus_tag="VM841_11190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HVL90784.1"
                     /translation="MGSKRLVALALALAAVAVAPAQGKPGVAKPAPGAAGTAAVAAAR
                     TGVLKIFSDYSNKAVVETVDAATGRLLSRTVDDESFEQPGEAHAGGVVAAAPSNAQRC
                     SNSGSWEEFYAGSPQPWRMSEQIRFNTGGVPSSVASSWRSMLRAAKGEWELTKNNCGV
                     GDLILWDMPEGSDTWTAPQCSSPDGENVVGFANLGGWSGGSLLLARTCVWFGGGYIYE
                     SDIAFNSHSAASWCNACTSTSSWDLQSVAAHEFGHMLGLGHTEHGATRETATAVMHAI
                     VYTGDSRNRTLSRGDINGAAALYPRLWGYEIQSTSLNNPAGAGVALEPNKTYTVTVDV
                     KNTGYLPWRVGDITGMRVATHPAGRCSGFVAADWASCSVASFIDEDLSNETDFEVNTS
                     AIVAQGEVARMRFTVPTTWAQEGVSSLERFMVRGGSVPVQDSSPFSFDVNVGTYAASV
                     VSTIKPGLPLLPGVIVRGTSSMGRITLRNDGSAPWWVENGRMRIVTSPEGRCSRFAGS
                     DWSSCSIASGIDRPETIVLPGETANFDMYFYANPNMQHGTSGIEVFDVEVVGRPVPRL
                     NRTASFRIEVW"
     gene            8525..8821
                     /locus_tag="VM841_11195"
     CDS             8525..8821
                     /locus_tag="VM841_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVL90785.1"
                     /translation="MRILLASSHIDDDRSLIGFLLVMALSGLVVGALGRLLVPGRQPM
                     GILATILAGVAGSVVGGFIARLIFGPEYVPGLIVGALGAALLIWAIYGTNRRHA"
     gene            8892..9548
                     /gene="nucS"
                     /locus_tag="VM841_11200"
     CDS             8892..9548
                     /gene="nucS"
                     /locus_tag="VM841_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HVL90786.1"
                     /translation="MRMIVAECSVDYRGRLSAHLPRARRLILIKADGTVVVHADKGHK
                     ALNWMSPPCSVTEADGIWTVTGAKGETLLIALHEIVSDVTVPLGDEPGLIKSGSELEL
                     QMLLAASPGVIEDGARLVQREYATDLGPVDLLLKDAGGGTIAVEVKRVGELPAVEQLS
                     RYLERLNRDASLAPVRGLLVAQTIKPQTRVLAAARGIGCVEVNIEVLAGRAEPDPTLF
                     "
     gene            9606..10004
                     /locus_tag="VM841_11205"
     CDS             9606..10004
                     /locus_tag="VM841_11205"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL90787.1"
                     /translation="MSSSSYVLTRAAAAAEAMHPEHVAPLVGKVLPHADADDAAEMFG
                     LLADPTRMRILHALGLAREICVYDLALLLGVGQSALSHQLRLLRDRNVVARRRDGRVV
                     YYSLADEHVRRMIRDAVGHSAEASPRRRRR"
     gene            10001..10669
                     /locus_tag="VM841_11210"
     CDS             10001..10669
                     /locus_tag="VM841_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HVL90788.1"
                     /translation="MTTITTAPSRPALLRTGLRLEYLTVGWNIAEGIVAVAAALASGS
                     VALLAFGVDSFVESVSGVVMIWRLRAERTHAPDPGSIERIERRARRLISVSLWLLATW
                     VVFDAAGTLIAADRPDVSGIGIAITSVSIGVMLWLARAKRAVAAALGSRAMQADAFQT
                     TACWWLSLTVLGGIALNAAFGWWWADPGAALVMTAFIGAEARAAWRGDDCCGDESPRP
                     GRAC"
     gene            10699..11343
                     /locus_tag="VM841_11215"
     CDS             10699..11343
                     /locus_tag="VM841_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL90789.1"
                     /translation="MPKISAETLEKHRAETIDKLVDSFGRLVMEQGYEDVSLADVAQD
                     VGLARTAIYNYFPDRESLLFAWTDREVRRAIEAMQRQIDEAPDSAAKLRVFVRLQLVD
                     FASRHLPPGQEVIQFLKPETYGRFMQHIEPIERLLRVVLAHGLETGEFADVEPDDVVP
                     LVIACIGSERGPLASGAHDVEEATDRVTSFLLRALHAPPEAAKKPGAPRKRSPL"
     gene            11428..12204
                     /locus_tag="VM841_11220"
     CDS             11428..12204
                     /locus_tag="VM841_11220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL90790.1"
                     /translation="MGALDGRVVVVTGASRGLGESLAIGYAQARAHVVLSARSKGDLD
                     RVAEACRDAGAASATVVPADVTDEAAVQSLVDATVAQHGTLDCFVANAGTSFAMLTDK
                     RYRELATWDAGIAEEILRVNVVGVLSCLKAAIPAMTAGGSFIAIGSETGRALRPGSGM
                     YAVSKAALDALATMAARETAETGIRVNVLSPGGMVDTQLFGPNGMPDFLKQMHPPLPP
                     DVIVPAAIWLASDDSDGVTGRFVSAKTFNEMGPAAAEAAR"
     gene            12204..13742
                     /locus_tag="VM841_11225"
     CDS             12204..13742
                     /locus_tag="VM841_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL90791.1"
                     /translation="MTVRPGGAALNISAGIREFAVATPGATAVVDGERALTYAALDDR
                     ARRFGNALLAAGLTPGDRVAVVLGNRLEYPEIAAGIARVGMQMVPVNPRLTAPEVTYI
                     VGHSEARATVVDESLSGTVAPALGEHDQRLVLSIDGTSLGPSYETALEKAPAADAGIE
                     VDEREPFCVAYTSGTTGRPKGVMISHRSRSLTFYGTALEWGLGPGRRTIAVAPMYHGA
                     GFAFGFGALHVGGELWMLRSFDAEPFLAMCAQSRAQSVFLVPTHAQMIRALGDDATMI
                     YDLTGLETLYFNAAPMPHPLKLWVADAFPHVALHELYGSTEAGIVSNCRPADVLRKPG
                     SVGPPWFMTEVRVVDEDGNPVAPGGTGELFSRSPFLMNGYLKDDEATLACTTPDGFLS
                     SGDVAREDEDGFLYIVDRTKDMIISGGTNVYPREVEDVLITHPEVRDVAVAGLPDETW
                     GEQVAAFVVVAPGSNAGDKELDAHCRASLAGFKIPRRYVFLDALPRNAAGKILKRELR
                     DRYA"
     gene            complement(13733..15094)
                     /locus_tag="VM841_11230"
     CDS             complement(13733..15094)
                     /locus_tag="VM841_11230"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HVL90792.1"
                     /translation="MRIAIAGAGGFVGFPLARRLAARSVDVVALGRTREALPSGPHID
                     KAVVDAADGDALQKALTGCDVAYYLIHSMTGEDFAARDRALASTFAQAAGAAGVPRIV
                     YLGALGRGELSEHLASRQETGRALGSTGIDVVELRAAVIIGAGSISFELMRYLVERLP
                     AMVCPRWVRTRIEPVSFDDVLAHLERAVSVPPGIYELGAGEVLTYQDLMQRYARIRGL
                     KPRAIIHVPLLSLNLSSYWVDVVTPVDKAVSHSLIASLAHEVVAHDRARTRETFGIDP
                     VPLDDAIIAALDEQRRRVAATLLDRPDGLADGVHTLSRRATIPPETTAAVRKDLTTIG
                     GSLRWYGIAAAWQARIWFGSTFGERLTLHKPARVERGADVDWWTIEVVSDDELVLRSR
                     EWAFGEAWLGWRVEDGTIRQTAAFRPRGVPGFVYWQVLRAIHHAAFDAMLRTRIRRAR
                     AQA"
     gene            15156..15740
                     /locus_tag="VM841_11235"
     CDS             15156..15740
                     /locus_tag="VM841_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HVL90793.1"
                     /translation="MRLGELLAPAPSRRWRRFGLPAPVAEAATRGEVAEILRGLPEGW
                     FVFNEIPAAASGSMIDHLVVGPGGAFAVTVADLGGQVRLAGRSLTVDGVASARLSKAV
                     LEARSVARALEAAGGRPVGVRPVVVVRCADLSIVSAPPDVTVLRPGSILRWLRTHPPV
                     LSRATVAAVARAAARPAAHAGSRSGSHARGASQQ"
     gene            complement(15765..17849)
                     /locus_tag="VM841_11240"
     CDS             complement(15765..17849)
                     /locus_tag="VM841_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90794.1"
                     /translation="MTAALSVRDLTVRIGETALLDGVSLEVGPGCIVAVVGPNGAGKT
                     TLLDAISGVAPGATGDVAIAGNDAASGTSGPRAGVGRVFQGSPLPETLTVGEVAAVKC
                     GGRAGAADLLARFGLMPHASSFVGELSTGMRRILDLAVATVGSPSVLLLDEPSSGLAQ
                     SEIEHLARLLRSWRDATGAAVLIVEHDASLVKAVADEVVVMDGGRLAARGPAAKVLKS
                     ARKGAMTRLHSPHEAHFKQALATVAAGASSAPPLVRRSLSTWTLLRLGLREFSAGLAS
                     VMILGVLNRVMKVELGISLAVTAAVLASYNFAAPFALMIGHRSDTKPLFGRKRTPYIV
                     GGALITGVCVIAAPHVAGQLQHGVTFGAVIASVALFVAMGTGMYGSGTVFFALLADIA
                     PPDERGHAASIVYLELMAGILAGVALTGAVVDDGAGNLGTLFALAGVLVVVFSTLAVW
                     GMEKHTRAPEPGEEPDEISVPFRRAVRGLAAIPQVRVFFAFMLLSTVFLFLQQAVLEP
                     FGGDVLGYSVRATSAFNAVMTIGIVVGMMLAGRPWAEQYGHTCIARWALMASVASFGG
                     LAAAAATSAAPPAWLSIMGIGLATGLFNVAVLALMMGMADKRRTALFMGTWTVAHAVA
                     DGTGVAGGGAIYELAQAVLNSVPGGYATVFALEAVGLALCLPLLRRVDPERFAQQARA
                     IDVDALAGDSGS"
     gene            complement(17846..19270)
                     /locus_tag="VM841_11245"
     CDS             complement(17846..19270)
                     /locus_tag="VM841_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL90795.1"
                     /translation="MRVAGSDTREAMATPTVRAAAFGAAFASAPGWAAAIVAPPAWDA
                     HLAMDERVAPWFAVALIISGVISATLAWRRGPETGAAALPGDAAQPYRIPPGFGALLM
                     LMFLSVVAIASADEVLVAMLLPLIPALSGWAAGQAARLAAGACVPSVLPAASGIVVAF
                     ALAGVFGGLHLARAGTGGGARDAGWWAIAYAAGAAINMIRARTSLDLDRKRIRARAAM
                     AAKQAEPVRHPGSGEPVLVMERMDLRFGANHVLREATMTVGAGELVALVGGNGAGKST
                     LLRVAAGLVTPGSGRILLDGDDVTTLRPEERSAAGLAFVSGARPVFPDLTVLENLRVA
                     AYRSHQTQKSFEQATDAILALAPTLQGRRRAKAGVLSGGEQRLLAVAQTLYRTPVALL
                     ADELTLGLDLDARLAVLDLLRVLADRGVAVVAVDHDLPSLLPRADRAALLTEGGVVLY
                     PEAAALLEQRQDLLPATFLAGAGL"
     gene            complement(19279..21177)
                     /locus_tag="VM841_11250"
     CDS             complement(19279..21177)
                     /locus_tag="VM841_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL90796.1"
                     /translation="MPPAGIIALGLVQGSLAGLNSLGLVLLWRTMRVINLAQATLGLV
                     GGVLVGLLVTQSQWSFWWAAPLGLAVGALLGLGSERIVLRRLQDAPRVVLLIATVGLA
                     SLFAAIQTALPFIFGGRLPTYTIDLGFSLELFPVKLLGPHLLALIVFPVALAATLHFV
                     HRSRIGLAALALGQDQERARTLGVPAGVVRGAVWAVAGALASVSGILSIPVLGFNIGG
                     GLGPTVLLLMLAPAVFAGLRSIAGTAAAALALGVGYQLILWYSPRAGVADLSLAAAML
                     VAVALQRRRLGRAEAAARASSWEAAAAPRPVPWRLASLPGVQLGGAFFAVAAIAVAAL
                     PPLVLSASSQVRFATTAAYAIAALAVAVAWMFAGEVVLGHWGVAGLGAAVAALTPGPW
                     VVRALVAAAAMGATGAGMALATRRQSSLSFAVLGLAAAASAPSVLLMVGDSTVPADVR
                     TVGMIGGGLTVALAAGLVRLRASVTGMRMVAARDDSQRAPWLGVNVMRSRVLALSISG
                     AMAGLAGSLYLAATPAGIEPGAFDPVWSLDLLAMAVIGGLGSPGGALLGALAIRAARI
                     FLPPEWAAVTSGAGVVLVVSFLPGGLGRGLQWLRDRAVRALAGPAGRDAPPGTPAPAE
                     APDPVNVP"
     gene            complement(21219..22529)
                     /locus_tag="VM841_11255"
     CDS             complement(21219..22529)
                     /locus_tag="VM841_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90797.1"
                     /translation="MSDNEILLAYYWKGDRTKTSPYLNQTGTGQEGNVDEGVAFMAMI
                     DWINKHATDGSEFLGYKFNLHGRKLKGFVKEAGQYPDDYAATGEFLARDLKLKGAAKP
                     GPFAAISSHGSLSAYICPRLAKAGIFNPSTYDLNWGLYQKTNGYCMSAGQSWSDQVEA
                     MIAYVTKQVNKTQYMTANVGQKRVYGLLYTEYPGLVDAAPKFERMIEKAGIDIALSLS
                     IAADLTTGQTMASTLANKFRDAGVNTLIIPDAGAPLNFTHAAQANGYSPDYLVWPCSG
                     QDNAAMVRLYNATQWTRAFGLTCYDEHFNPDLTNDGIAKQTEWWKMFKEVRPNTEPPS
                     PTAFVAQSLIPILAGITDAGRDLTLESFRVALSMFKPYRYDAIEGRTTNPRSMLITID
                     MSQGGQIGDATKLRWNPTKRNPGSNHAGAYDYPESKRYRRASDY"
BASE COUNT         3524 a         7807 c         7851 g         3457 t
ORIGIN      
        1 cgacgcgcat catctggtgc ccggccgcgg ccgccaggtc accgacctcg tgctcctttg
       61 ccgccgatgt caccgaaggg tccaccgctt caagtggaag gtccgtatcc aagccgacgg
      121 agcaatcacc ttcaccgccc gcggccgcac ccacaccagc cacccccgcc accgctcccc
      181 accagcgctc gaatagcagt cggtcgattc cgttggaggc ctttgccggt cgcccttctg
      241 ggcgccggca atccaccagg agcgcagagc tttcagaacg ctgtgaaaat gttgcgagcc
      301 tgtccaatgt cgcttgcagc aagccgctga gggcggagtg cgacctttac ggcgcgtgga
      361 cctggtccaa gccgaaaccg aagacgtcgg ccgtgttccc gaccgatctg gtcccgatcc
      421 acacgcgggc attcccgcgg cccttgcatg cgccggacgt cggcatcgtc ggcaacacct
      481 gagcgggcac cttcatcgcc ggggaggtcg cacacgtgta gccgtccacg tccgacgcgc
      541 cgacatctat gaatatccct tcaccgtcgt aggccgaggc tccgaaacac cacgcagaag
      601 cacagttgta ggaatccaca ccgccggcat cgaacaggat cgcggctgcg ccgctgcccg
      661 acgcatggct catcccgccg atcccatctc cgtagtaggt gtcgtttccg gggccgcggt
      721 cgacgaccat tccgagcgtg cccgcggacg cggcagtatc tctgtcgtcg ggaccccagg
      781 ccacggccac gcagaaatct ttcttattcg tgcctgtgca ccgataactg tcggttccgc
      841 ccggatccag cacaacggct ttcgagcctg acgtcgcgac cccgatcgac catccgtagc
      901 catagatgtt cgtgtcggta ttgacgttgc ggtaggtcgt attgcctgcc ccaccgatca
      961 cgagtccgag tccctctccg tcgccgtagc cgatgttgta catccctttg gcgtcgaacg
     1021 ttgttggtga ttgatcggct gcaggatcga taaggatgcc gatcccgcca ttggttccga
     1081 cacccatgag accattagga ccttgcaggt tttggttggt cgcactgaaa tacgaggaat
     1141 acgtgtcgct tccgttgccc aggtcgagca gcaggcccaa gcccctcttc cccatgcctt
     1201 gggaacacga atagcctgtt cgtgaatcag cgccgtcggc atcgatcagg atcccgaccg
     1261 cgttccacgt cccggcaccg atgctggggc ctggttgctc gtcgcacgca cggaagctat
     1321 gcgtcgagcg gccgtaagtg tcgttgccgg ccaggtcgac gactgccgcc agagcagtca
     1381 tgatgttcgt cccgagcgcc ccgcctgcgt tgttggggta cgtgtcgttg ccgcccggct
     1441 cgataatcag gatcgcatgg ttggtgtatg tgtcgtttcc ggttccaccc acaacgaact
     1501 tatccagaat gtccagactt accgcttccg ccggccacgt cgcgccggca gggatggatt
     1561 tgatcgccca agagatgtct cgcgatgcct cgagccatgc ggtctgttgc tctgcgagcg
     1621 aagcgttcgc gaccgatgcg atcaccggaa catatcgcgc gaagcattca acgatgcggg
     1681 ccgtcgctgc cgatacctca gacgatagcg tgataccgga tcgcattgag gtatcagttc
     1741 ccaccgccgc gccgaagtgg tcgagcgcgg tcgcaagggt cggcggggtc ccttcgcaac
     1801 ggttcgaggt caacggcagt cccatcaaca cagccacttc cgctgggacg tcgtatgggt
     1861 cgacgtccat caggcgctcc cacgcgaggc gctccgtttc gtgcaggctg agcgtggtga
     1921 tcgggtccgg cgtgcaagca tatgaattcg ccgaaggatc gctgcacgtt gggaaggcgg
     1981 cggttgtcgc cggcgtcggg ttgagccgca tccgcaccga gcccgcgtcg gcgttcttcg
     2041 ggatcaacgc ggaaagggtc tgtgtccgcc cacttccatt ggatatcgtt tgagtcacga
     2101 cgcccgacgt gttgatgcgg aacgacgtgt cgaaggtcgc cgagctctct cgaaggttcg
     2161 tgctccagac gatgccgccg gtcgtgtacg tcacctcagc aaactgcggg ttgaatgtga
     2221 tccgcgttgc accgatgccg tcgtgtttcg tgctggcgaa ttggaccgtg taggaccctg
     2281 cggaaaccaa cggtgttgcc cactgataca ggtcgagcga agtgttgtag ctgatcctcg
     2341 tcgtcgccgg ctgttggggg atgctcacgt tcggtctcga caccgcagat gtacccataa
     2401 ccaagaagcg tcccttcatg gtcgtggggt ttccggcgtt gtcggaaacg gtcgcctgga
     2461 cctcgtactc gcgcccagga atcagggtca caggctgcga cttgatgctg attgtggtgt
     2521 ctccggtatt cgccgattca gcgacgaatc ctggcgtttg gaacgcggcg aaattctgca
     2581 gatcgaccac tttcatcgtc atcgtcgagg tgttcgctcc cgaggtggtg ggagccacac
     2641 ccgccgacgg cggcggagca tcgatgatct gcagcaggat ggggagactc gtgtaggcga
     2701 cgttgcctcc cttgcgcgga ctgatcatgc tgatcctcgg tggggtcaaa tcgatagacg
     2761 tcgtcacggt cgcgagcgcc gtgccgttcg cgtcggtcgc gctcagctgt accgaatagt
     2821 ccccgtctgg aagggcgttg ttttcctcgt ccaggccatt ccatgactga gcgacggagg
     2881 ctccgctttg cgagaaggtc ctgacgatcg cgcccgacgc gttggtgatg gtcagggtcc
     2941 aagtcggcgt cccttgtgca ttgatggaag ccgagatgac cgtggcgtcg cgggtgccgt
     3001 caccgttcgg gctgaacacc aacggcgaag cggcgaagct cgtgatcgaa ggcgatgcga
     3061 atgccggcgc cgctccgctg atcagcagga cgcctgacat aaggcttgca agagcaatgc
     3121 ggacaggttt cgtcataccc gactcttccc ccttcgaccg attagggacg aattatgacc
     3181 ccgaagtcgg ccttaggaca agaggagccc ggctcgatat ggtcgtcgga tgaatccgga
     3241 cgtcctacgc gcagccgtag gagccggcgg gccagcgttc gatgcggtcg tcggagacca
     3301 gatagatcgc ggtatcgtcg gtcggcacgt accacagcgc ggcatccggg ccgacgagcc
     3361 tggtgaactg cgcctcgtcg gggagacgat cgatccgctc cgctttcttg gtgttccctg
     3421 caatcacgcg ctcggcatcg ttcagaaaga tgcgcacatc gcctcggccc ggcgtgtcgg
     3481 gcaccgagcc cggcggccag gtcgtcacga tgtctcgcac ggactgaaac ccgcaatgct
     3541 cgggtccggt ccttgtcatg acgacgtcga gcctgacggg gcacccatcg gccgtcctcc
     3601 accgctttag gctgccgccg tcggagtaat ccggttcctg tgccaggtcc gtgatcgagc
     3661 cgggcgacgg cgcacggcac gccgatggca tgggctcggc ggtgcacgaa accagcatcg
     3721 ctgccgtcag cgtcgacgcg gcggcggagc atgcgatccg aagccggacg tccatgatcg
     3781 aagcatatcc ggcaggcccg gtttcgctag agcggaatct tgaacagcag cccttcgccg
     3841 agcatccacg tgtcgaagaa cagatgcggg tcgcggcgtg catcgaagcc gccgtcgccg
     3901 tccccgtcca cccacacgcc gcgcgcacgc gcatccaact gcttgccggc gtcgcgaagc
     3961 tgcgagtcca ggtagccgta cgccgagccg gccgcgctcg ggaccggcac gaacacttgc
     4021 gcatcctcgc gggcctcgta ccggccgcta tccgaggtgt ccccccatat cccgtgcacc
     4081 ggcgagccgg cgtcgttgta gtgggcgacg ccgttcgccg tcaggaaccc gcccggatac
     4141 gcaaaatagt ccggctcggg agcgtggccg agcatgcgcg atgcctgctg cgagagcgcg
     4201 cgcgcggtgt cgcgtccgag cgagagcgtc tcttgatagt gagagacgcc gttgcggtcc
     4261 acgccgttcg aatacagggg attcaaggtc ggcggtacgt agtcggtggc cgacacgaag
     4321 tagccgagct catccatgcc gagcccgacg acgaagtgga tgtcgcccgt catctgcggc
     4381 tcgatcgcgc gctcgggcgg cacgtcgggg ttcgcgcgct cgtgcatcgg ctggtacacg
     4441 ccggtgtaga ggtcgggctg aagctcgccc ggcatcgtga ggaactcggc gggaccgaag
     4501 cgcgcgtgca gcatctcggt cttgacgtca cggcccgccg ggctctccac gcccgcggta
     4561 taggtcggcc ggtccaccac gcccgcggcc ttgagcgcaa ggatgaaggc gttctcgacg
     4621 gtcagaaaca tctcctccga ccggacggcc agcgtcggcg cggtgacgga cagagccgac
     4681 ggcgcggcca gcgcgtccaa ggcggtgagc ccgatccggc ggccgacgtg ataggccttc
     4741 ttgacggact tcttcgggaa gagcgtgccc gtgtcgggat cgacgatcgc aactgcgagc
     4801 ccggtgaccg ccccgttcgg cccgttcagg tacacggtcg tgccgccgcc cgcggcctcg
     4861 acggtggtgc gcagcgtgtg cgcgtagtcg ctggacatga ccgcgccgcg catcgatgcg
     4921 gggatcatcg ggtcgctggt ggacttggag tcgatcagct cgggatggct cgcccagttc
     4981 acgatggttc cgatcgcccc gctcgccccg cgaagctgga tcacgttgac gctgtcgtcg
     5041 aaagtgccgg gatggcggct gcggtcgtac tcgttgacgg tctcgccggg aaccttcgag
     5101 ccgaatcgga cctcggtcac gtcctgcgcc gcggctgccg cgtcgatcgc cgcgccggcg
     5161 gcctgcgaga gcacaaacgc ttgatacgcg ggattgatcc cggacgtggt ctggtccggg
     5221 ccgtagatgc cgagcgtgtc gatgctcgaa tgcgtgtgcg tggacgcgac cagcacgtgc
     5281 gtgccggccc cgagcgcgcc gcggatctgt tcgcggatcc gaaccacgtc ctcgtagaac
     5341 aggcccaaga cgtccagcga cacgatcgcg agcgacgtgt ccccgctgcg aatgaaggac
     5401 gcgcgcgccc acagcccgtc cccgggcagc acgccggtcg gccggcggcg cgtcgtctgc
     5461 cagacggcgc tccagtcgga ctgggccgga acgatggagc gcacggcgaa gcccgcacga
     5521 acgggcccga ccggcacgga ggacgcgaaa cccaaggccg ggacgaggag aagcaagggc
     5581 aaaaagaagc ggcaaaatcg catgtcgaac ccccccgcag acccctttcg ccacagacgc
     5641 gcacggatcc ttcatcgctt gcttgttgcg ttgatgtgca acgccctata gtcgaaccat
     5701 gagccgcacc gtcgggccgg tcccggacac gagcgggctc ggcgccggag atcacgtctg
     5761 ctgggtctac gcagacgacg aggaccaccg ggccggggtc acccgctact ttgccgacgg
     5821 gatcgaacgc ggtgagcgga tcgtctacgt ctcggaccac tacgacgaag ccaccgtcgt
     5881 ggcgtaccta tcggcggccg gtttcgcccc caagcgcatg ctcgagcaag gaagcctgat
     5941 cgtgatgcct gcgtgcgaga gctacccgcg cgacgagcac ggctacgacc tggacgccac
     6001 cgtggcgcag ttccaggcat tcgctcagca ggcggtcgcg gacgggtata ccgggtttcg
     6061 cgtcgccggt gaatccgact tcctgctcga gaccgtcccg cagcacggcc tcctggccta
     6121 cgagctcacc gccgacctga tggtccgcga ggctcccatg acgggcatgt gcctctacga
     6181 cgcgcggcac gctccgatgg aacgtctcgg cgaggtcgcg gcggtgcacg cggccgccgt
     6241 cgtggcgtcg tcgctcgcgg cggatctgcc gtcgtttcgg ctctgggcct ccgaaaccga
     6301 gacgatcggc ctcgccggcg agatcgactt cgccaccgcg gacaccgtgc ggcagatttt
     6361 gatggatggc gcggtgggcc gcgccggcgt gctggacctg acgacgctgg gctttgccga
     6421 cgtcgccgcg atccgcgcca tcgcccgggt ggcgcaagcg atggccggcc agaacgaccg
     6481 gccggtcatc cggggagctg cttcccaggt tcgcttcgtg ctctcgcagt tcgaggtcag
     6541 ctccctcgtt ttcgacggct gagcacgccg cattcggaca taccgggcgc ctccgccttt
     6601 ttctgcggtg aatcccggcc gcaccgaacc accaagtgat atcgaagggc tgcttgatgc
     6661 cgtctcggcc gaaacgcgcc acctccaatc ggcaggaacg agggcggggc aaacgaactg
     6721 aagtgctgac gggtctgggg ggacatcgtg gtgggatcca aacggttggt cgcgctggcg
     6781 ctcgcgctcg ccgcagtggc ggtcgcgccc gcgcaaggaa agccgggagt ggcgaagccc
     6841 gcccccgggg cagccgggac cgccgcggtc gctgctgcgc gcacgggcgt gctcaagatc
     6901 ttcagcgact acagcaacaa ggccgtcgtc gagacggtcg acgcggccac cggcaggctg
     6961 ctgtcgcgga ccgtggacga cgagtctttc gagcagccgg gcgaggcaca cgccggcggt
     7021 gtcgtggccg ccgcgccttc gaacgcccag cgctgctcca acagcggttc gtgggaagag
     7081 ttctacgccg gctcgccgca gccgtggcgc atgagcgagc agatccgatt caacaccggc
     7141 ggcgtgccgt cctcggttgc ctcgtcgtgg cgctcgatgt tgcgggccgc caagggcgaa
     7201 tgggagctga ccaagaataa ttgcggcgtc ggcgatctga tcctgtggga catgcccgag
     7261 ggcagtgaca cctggacggc gccgcagtgc tccagccccg acggtgagaa cgtcgtgggc
     7321 ttcgcgaacc tgggtggctg gagcggcgga agcctgctgc tggcgcgcac ctgcgtgtgg
     7381 ttcggcggag gctacatcta cgaatccgac atcgccttca acagccattc ggccgcgtcc
     7441 tggtgcaacg cgtgcacgag cacgtcgtcg tgggatctgc agtcggtggc cgcgcacgag
     7501 ttcggccaca tgctgggcct cggccacacc gagcacggcg cgacgcgcga gaccgccacc
     7561 gcggtgatgc atgcgatcgt ctacacgggg gactcgcgca accgaaccct gagccggggc
     7621 gacatcaacg gagcggccgc gctgtatccg cgtctgtggg gatacgagat ccagagcacg
     7681 tccctgaaca accctgccgg ggccggcgtc gcgctcgagc cgaacaagac ctacaccgtc
     7741 accgtggacg tgaagaacac gggctacctg ccgtggcgcg tgggtgacat caccggcatg
     7801 cgggtcgcga cgcatcccgc cggccgctgc tccggcttcg tcgcggccga ctgggcttcg
     7861 tgctcggtcg cgtccttcat cgacgaggat ctgtccaacg agaccgactt cgaggtcaac
     7921 acctccgcga tcgtcgcgca gggcgaggtc gcccggatgc gcttcaccgt gccgacgacc
     7981 tgggcgcagg agggcgtctc ctcgctcgag cgattcatgg tgcgcggcgg ctcggtgccc
     8041 gttcaggact cgtcgccgtt ctccttcgac gtcaacgtcg gtacgtacgc ggcctcggtc
     8101 gtgagcacga tcaagccagg ccttccgctg cttccgggcg tgatcgtgcg cggcacctcg
     8161 tcgatgggcc gcatcacgct ccgcaacgac ggaagcgcgc cctggtgggt ggagaacggc
     8221 cgcatgcgga tcgtgacctc tcccgagggc cggtgcagcc ggttcgcggg cagcgactgg
     8281 tcctcgtgca gcatcgcatc gggcatcgac cgtcccgaga cgatcgtgct gccgggcgag
     8341 acggcgaact tcgacatgta cttctacgcc aacccgaaca tgcagcacgg cacgtccggg
     8401 atcgaggtct tcgacgtcga ggtggtcggg cggccggtcc cgcggctgaa tcgaacggcc
     8461 agcttccgca tcgaagtctg gtagagccgg ttaggaaccg gagcccggcg ggtatgttca
     8521 tcgcatgcgg atcctgcttg cttcttcgca catcgacgac gaccggagcc tgatcgggtt
     8581 cctgctcgtc atggcgctca gcggcttggt cgtcggcgcg ctcgggcggt tgctggtgcc
     8641 cggccgccag ccgatgggca tcctcgcgac gattctggcg ggcgtcgcgg gatccgtcgt
     8701 cggcggcttc atcgcgcgac tgatcttcgg acccgagtac gttcccggcc tgatcgtggg
     8761 ggcgctcggc gcggcgcttt tgatctgggc gatctacggg acgaaccgga gacacgcctg
     8821 atccaatgcg ggccggtgtc cccgtgccgg cccgcaccca ggctttcgat cccggtaggc
     8881 tacgggccct catgcgcatg atcgtggccg agtgctccgt ggactatcgg ggccgcctct
     8941 ccgcccatct gccgcgcgcg cggcgcctga tcctgatcaa ggccgacggc accgtcgtcg
     9001 ttcacgccga caaggggcac aaggcgctga actggatgtc gccgccctgc agcgtgaccg
     9061 aggccgacgg catctggacc gtgaccggcg cgaagggaga gacgctcctg atcgcgctgc
     9121 acgagatcgt ctccgacgtg accgtgccgc tcggggacga gccggggctg atcaagagcg
     9181 gcagcgagct cgagctgcag atgctgctcg ccgcctcgcc cggcgtgatc gaggacggcg
     9241 cgcgcctggt gcaacgcgag tatgcgaccg acctcgggcc ggtggacctg cttctcaagg
     9301 atgcgggcgg tggaacgatt gcggtcgaag tcaagcgggt cggcgagctt cccgcggtcg
     9361 agcagctttc tcgctatctc gagcggctca accgggacgc gtcgctggcg ccggtgcgcg
     9421 ggctgctcgt ggcgcagacc atcaagcccc agacgcgcgt actggcggcc gcacggggga
     9481 tcggctgcgt cgaggtgaac atcgaggttc tggccggccg cgccgagccg gaccccaccc
     9541 tgttctgacc gccgggattc tccggccctt gaccgggctc gcttcccacc tgtttaattg
     9601 aacatatgag cagtagttca tatgttttga cccgtgcggc ggccgccgcg gaggcgatgc
     9661 atcccgagca cgtcgccccg ctcgtcggca aggttctccc gcacgcggac gccgacgacg
     9721 ccgccgagat gttcggcctt ctggccgacc cgacgcgcat gcgcatcctt cacgccctcg
     9781 gtctggcccg cgagatctgc gtctacgacc tcgccctgct gctgggcgtg gggcagtccg
     9841 cgctgagcca tcagctccgg ctgctgcggg accgcaacgt ggtagcccgc cggagggacg
     9901 ggcgcgtcgt gtactactcg ctcgccgacg agcacgtccg gcgcatgatc cgcgacgcgg
     9961 tcggccacag cgccgaggca tcgccccgga gacgccggcg ttgacgacga tcaccacggc
    10021 accgtcccgg ccggcgctgc tgcgcaccgg ccttcgactg gagtacttga cggtcgggtg
    10081 gaacatcgcg gaggggatcg tcgcggtcgc ggcggccttg gcctcgggca gcgtcgcctt
    10141 gctcgccttc ggcgtggaca gcttcgtcga gagcgtctcg ggcgtcgtga tgatctggcg
    10201 gctgcgagcc gagcgaacgc atgcgccgga tcccggcagc atcgagcgca tcgagcggcg
    10261 cgcccggcgc ctgatctcgg tgtcgctgtg gctgctggcg acctgggtgg tcttcgacgc
    10321 agcgggcacg ctcatcgccg cggaccgccc ggacgtgtcc gggatcggga tcgcgatcac
    10381 gagcgtgtcg atcggcgtga tgctgtggct cgcccgcgcc aagcgcgccg tcgccgccgc
    10441 gctggggagc cgagcgatgc aagcggatgc gtttcagacg acggcgtgct ggtggctctc
    10501 gctcaccgtg ctcggtggga tcgcgctcaa cgccgcgttc ggctggtggt gggcggaccc
    10561 gggtgcggcg ctggtcatga cggcgttcat cggcgcggag gcgcgggccg cgtggcgcgg
    10621 cgacgactgc tgcggggacg aatcgccacg gcccggacgc gcctgctgac gcccggtcgc
    10681 gccgatatac tcctgcccgt gccgaagatc tccgccgaga cgctcgaaaa gcaccgcgcc
    10741 gaaacgatcg acaagctcgt cgactcgttc ggacggctgg tgatggagca gggctacgaa
    10801 gacgtctcgc tcgccgacgt cgcgcaggac gtggggctcg cccgcaccgc gatctacaac
    10861 tacttcccgg accgcgaatc gctgcttttc gcctggaccg accgggaggt ccgccgcgcc
    10921 atcgaagcga tgcaacgcca aatcgacgag gcgcccgaca gcgccgcgaa gctgcgcgtg
    10981 ttcgtgcggc tgcagctcgt ggacttcgcc tcccggcacc tgccgcccgg gcaggaggtc
    11041 atccagttcc tcaagcccga gacctacggg cggttcatgc agcacatcga gccgatcgag
    11101 cgcctgctgc gcgtcgtgct ggcgcacggc ctggagaccg gcgagttcgc cgacgtcgag
    11161 cccgacgacg tggtcccgct cgtgatcgcg tgcatcggct ccgagcgcgg gccgctcgct
    11221 tcgggcgcgc acgacgtgga ggaagccacc gaccgggtga cctcgttcct tctgcgcgcg
    11281 ctgcatgccc ccccggaggc cgccaagaag cccggcgcgc cccgcaaacg ctcccccctg
    11341 tagccgttcg ccccgcggga tcgcgcgacc tctctgccat aatgtcgggc cgtcgccgga
    11401 acgatgtttg gtaagggagg aacggcaatg ggtgcgctgg acggaagggt cgtcgtcgtc
    11461 accggagcca gccgcgggct cggcgagtcg ctcgcgatcg ggtacgccca ggcgcgcgcc
    11521 cacgtggtgc tttcggcccg cagcaaaggc gatctggatc gggtcgccga ggcctgccgc
    11581 gatgccggcg cggcatccgc gacggtcgtg ccggccgacg tgaccgacga ggccgccgtg
    11641 caatcgctcg tggacgcgac cgtcgcgcag cacggcacgc tcgattgctt cgtcgccaac
    11701 gccggcacct cgttcgcgat gcttaccgac aagcgctacc gcgagctcgc gacctgggat
    11761 gccggcatcg ccgaggagat cctgcgcgtc aacgtcgtcg gcgtgctgtc gtgcctcaag
    11821 gctgccatcc ccgcgatgac ggcaggcggg tccttcatcg cgatcggatc cgagacgggc
    11881 cgcgcgcttc ggccggggtc cggcatgtat gcggtctcca aggcggcgct cgacgcgctg
    11941 gccacgatgg cggcgcgcga gaccgccgag acgggtatcc gcgtcaacgt gctctcgccc
    12001 ggcggcatgg tcgacacgca gctgttcggc ccgaacggga tgcccgactt cctcaaacag
    12061 atgcatccgc ccctgccccc cgacgtgatc gttcccgccg cgatctggct cgcctccgac
    12121 gactccgacg gcgtcacggg ccgcttcgtg tcggcaaaga cattcaacga gatgggcccg
    12181 gccgcggccg aagcggcacg ctgatgacgg tccggccggg cggagccgcg ctcaacatct
    12241 ccgccggcat ccgggagttc gccgtcgcca cgccgggagc gaccgcggtc gtggacggcg
    12301 agcgcgcgct cacgtatgcg gcgctggacg accgggcccg gcgcttcggc aacgcgctgc
    12361 tcgctgcggg gctcacgccg ggtgatcgcg tcgccgtcgt gctggggaac cgtctggagt
    12421 atcccgagat cgcggcgggc atcgcccggg tcggcatgca gatggtgccg gtgaacccgc
    12481 gcctgaccgc ccccgaggtg acctacatcg tcggccattc cgaggcgcgc gcgaccgtcg
    12541 tggacgagtc gctgtccggg accgtcgctc cggcgctcgg cgagcacgac cagcggctgg
    12601 tgctctcgat cgacggcacg tcgctcggac cgtcgtacga gacggcgctg gagaaggccc
    12661 cggccgccga cgccgggatc gaggtggacg agcgcgagcc gttctgcgtc gcctacacgt
    12721 ccggcacgac cggccggccg aagggcgtga tgatcagcca tcgatcgcga tcgctgacct
    12781 tctacggcac ggcgctcgaa tgggggctgg gcccgggccg acgcacgatc gcggtcgcgc
    12841 cgatgtatca cggcgccgga ttcgcattcg ggttcggtgc gctgcacgtc ggcggcgagc
    12901 tatggatgct gcgctcgttc gatgccgagc ccttcctcgc gatgtgcgcg cagtcccgcg
    12961 cgcaatcggt gttcttggtg ccgacccacg cgcagatgat ccgcgccctc ggcgacgacg
    13021 cgacgatgat ctacgacctg accgggctcg agacgctgta cttcaacgcc gcgccgatgc
    13081 cgcatccgct caagctctgg gtcgcggacg cgttcccgca cgtcgccctt cacgagctct
    13141 acggctcgac cgaggccggg atcgtgtcga actgccgccc ggcggacgtg ctgcgaaagc
    13201 ccggatcggt cggcccgccg tggttcatga ccgaggtgcg cgtcgtggac gaggacggca
    13261 accccgttgc gcccggcggc accggcgagc tgttcagccg ctcgccgttc ctgatgaacg
    13321 gctacctcaa agacgacgag gcgacgctgg cgtgcacgac gccggacggg ttcctgtcca
    13381 gcggtgatgt cgcgcgagag gacgaggacg gattcctcta catcgtggac cggaccaagg
    13441 acatgatcat cagcggcggt acgaacgtgt atccgcgtga ggtcgaggac gttctgatca
    13501 cccatcccga ggtgcgcgac gtggccgtcg ccggcctgcc cgacgagacc tggggcgagc
    13561 aggtcgccgc cttcgtcgtg gtcgcgcccg gctcgaacgc cggagacaaa gagctcgacg
    13621 cgcactgccg cgcgtccctc gccggcttta agatccccag acggtacgtg ttcctggacg
    13681 ccttgccgcg caatgcggcc ggcaagatcc tcaagcgcga gctgcgcgac cgctacgcct
    13741 gagcgcgcgc ccggcggatc cgggttcgca gcatcgcgtc gaacgcggcg tggtggatgg
    13801 cgcgaagcac ctgccagtac acgaatccgg ggacgccgcg cgggcggaat gcggccgttt
    13861 gccggatggt tccgtcctcg accctccatc cgagccacgc ctcgccgaac gcccactcgc
    13921 gcgagcggag cacgagctcg tcgtccgaga cgacctcgat cgtccaccag tccacgtcgg
    13981 cgccgcgctc gacacgagct ggcttgtgca gggtgagccg ctcgccgaag gtggagccga
    14041 accagatgcg agcctgccat gcggccgcga tcccgtacca gcgaagcgac ccgccgatcg
    14101 tcgtgaggtc tttgcgtacc gccgccgttg tttcgggcgg gatcgtcgcg cggcgcgaca
    14161 gggtgtgcac cccgtccgcg agtccgtcgg gccggtccag cagcgtggcc gcgacgcgcc
    14221 gccgttgctc gtcgagtgcg gcgatgatgg cgtcgtcgag cgggaccgga tcgatgccga
    14281 aggtctcgcg cgtgcgtgcc cggtcgtgtg cgacgacctc gtgggcgagg cttgcgatca
    14341 gcgaatggct caccgccttg tccaccggcg tgacgacgtc cacccagtag ctcgacaggt
    14401 tcagcgaaag cagcggcacg tggatgatcg cgcgcggctt cagaccgcgg atgcgcgcgt
    14461 accgttgcat gagatcctga taggtcaaaa cctcacccgc cccgagctcg tagatgcccg
    14521 gcgggacgga caccgcgcgt tccaggtgcg cgagcacgtc gtcgaacgag accggctcga
    14581 tgcgcgtccg cacccatcgc gggcacacca tcgcgggcag gcgctcgacg aggtagcgca
    14641 tcagctcgaa cgagatgctg cccgcgccga tgatcacggc cgcgcgaagc tcaactacgt
    14701 cgatgccggt cgatcccagc gcccggccgg tctcctggcg gctggccaga tgctccgaga
    14761 gctcgccgcg tccgagcgcg ccgagataca cgatgcgcgg cacgccggcc gcgcccgccg
    14821 cttgggcgaa cgtcgaggcg agcgcgcggt cgcgggcggc gaagtcctcg cccgtcatcg
    14881 agtggatcag gtagtaggcg acatcgcatc cggtgagtgc cttctgcagc gcgtcgccgt
    14941 cggccgcgtc cacgacggct ttgtcgatgt gcgggcccga cggaagcgcc tcgcgggtcc
    15001 ggccgagcgc caccacgtcc acgctccgcg cggcgagcct tcgggcgagc gggaaaccga
    15061 cgaatccgcc ggctcccgcg atcgcgatgc gcacggaggt agtgtagacg cggaaccgct
    15121 caagccgggc cccttccacg ccgataggtc cgggcatgag gttgggggag ttgctcgcgc
    15181 cggcgccgtc gcgccgatgg aggcgtttcg gcctgccggc accggtcgca gaggccgcga
    15241 cccggggcga ggtcgccgag atcctgcgag ggctgcccga aggctggttc gtcttcaacg
    15301 agattcccgc tgcggcctcc ggctcgatga tcgatcacct cgtggtcggg cccgggggtg
    15361 cgttcgcggt gaccgtggcc gacctcggcg ggcaggtgcg tcttgccggg cggtcgctca
    15421 cggtcgacgg ggtggcctcg gcacggctgt cgaaagccgt tctggaggcc cgcagcgtcg
    15481 cgcgagccct cgaggcggca ggcggtcgtc cggtcggggt tcgtcccgtc gtcgtcgttc
    15541 gatgcgctga cctgtcgatc gtttccgcgc cgcccgacgt cacggtgctg cggcccggaa
    15601 gcatccttcg gtggcttcgc acgcacccgc cggtcctttc tcgcgcgacc gtcgccgccg
    15661 tcgcgcgggc ggccgctcgc cccgctgccc acgcaggcag tcggtcagga tcccacgcac
    15721 ggggggcgtc ccaacagtga cccggtgagg gatatgtttt tgggctacga gccggaatcg
    15781 cccgcgagcg cgtcgacgtc gatggccctg gcctgctggg cgaagcgctc cgggtccaca
    15841 cggcggagca ggggcaggca caacgcgagg ccgaccgctt cgagggcgaa gaccgtcgcg
    15901 tatccgcccg gcacgctgtt gagcaccgcc tgcgccagct cgtagatcgc cccgccgccc
    15961 gctacgccgg tgccgtcggc gacggcgtgc gcgacggtcc acgttcccat gaatagggcg
    16021 gtgcgccgct tgtcggccat gcccatcatc agtgcgagca cggcgacgtt gaacaggccc
    16081 gtcgccaggc cgatgcccat gatcgacagc cacgcgggcg gcgccgccga cgtcgcggcc
    16141 gcggcggcca gcccgccgaa cgaggcgacc gatgccatca gcgcccagcg cgcgatgcac
    16201 gtgtgcccgt actgctcggc ccacggccgg ccggcgagca tcatgcccac gacgatgccg
    16261 atggtcataa ccgcgttgaa cgcgctcgtg gcacgaaccg aatagccgag cacgtcgccg
    16321 ccgaagggct cgagcacggc ctgctgcaag aacagaaaca ccgtggacag cagcatgaac
    16381 gcgaagaaga ctcgcacctg cgggatcgcg gcaaggccgc gcacggccct gcggaacggg
    16441 acagagatct cgtcgggctc ctcgccgggc tcgggcgcgc gcgtgtgctt ttccatgccc
    16501 cagacggcga gcgtcgagaa caccaccacg agcacgccgg cgagcgcgaa gagcgtgccg
    16561 aggttcccgg cgccgtcgtc gacgaccgcg ccggtcagcg ccacgccggc caggatgccg
    16621 gccatcagct ccagatagac gatcgacgcg gcgtggccgc gctcgtcggg cggcgcgatg
    16681 tcggcgagca gcgcgaagaa caccgtgccc gacccgtaca tacccgttcc catcgctacg
    16741 aacagcgcga ccgatgcgat cacggcgccg aacgtgacgc cgtgctgaag ctgtccggcg
    16801 acgtgcggag cggcgatcac gcagacaccg gtgattagcg ctccgccgac gatgtagggc
    16861 gtgcgttttc gtccgaagag cggcttggtg tcggagcggt gaccgatcat cagcgcgaac
    16921 ggcgcggcaa agttgtagct ggcgagcacc gcggccgtga cggccaggct gatgccgagc
    16981 tcgaccttca tgacgcggtt gagcacgccc aggatcatca cgctcgccag gcccgccgag
    17041 aactcgcgca gcccgaggcg aagcagcgtc caggtcgaca gggaacggcg caccagcggc
    17101 ggcgcggatg acgcgccggc cgcgacggtc gccagggcct gtttgaaatg cgcctcgtgc
    17161 ggcgagtgga gccgcgtcat cgcacctttg cgtgcgctct tcaagacctt cgcggccggc
    17221 ccgcgcgccg cgagccgtcc gccgtccatc acgaccacct cgtcggcgac ggccttcacc
    17281 agcgacgcgt cgtgctcaac gatcagcacg gccgcgccgg tcgcgtcacg ccaggatctc
    17341 agcagccgcg ccaggtgctc gatctcggac tgcgccaggc cgcttgaggg ctcgtcgagc
    17401 agcagcacgg acggcgatcc gacggtggcc acggcgagat ccaagatgcg ccgcattccg
    17461 gtggacagct cgccgacgaa cgaggaggcg tgcggcatca ggccgaagcg ggcgagcagg
    17521 tctgccgcgc ccgcgcggcc gccgcacttg accgccgcga cctcgccgac ggtgagcgtc
    17581 tcgggcagcg gcgagccttg gaaaacccgc ccgacaccgg cacgcgggcc gctggtgccg
    17641 gacgcggcgt cgttgcccgc gatcgccacg tcaccggtgg cgccgggcgc gacgcccgag
    17701 atcgcgtcga gcagcgtggt cttgcccgcg ccgttcgggc cgacgacggc cacgatgcat
    17761 ccggggccga cctcgaggct cacaccgtcg agcagcgcgg tctcgccgat gcgcacggtg
    17821 aggtcgcgga ccgacagggc cgccgtcaca ggcccgctcc cgcgagaaac gtggcgggca
    17881 gaagatcctg ccgctgctcg agcagcgccg cggcctccgg gtagagcacg acgccgccct
    17941 cggtcagcag cgccgcgcgg tccgcgcgcg gcagcagcga cggcaggtcg tgatcgaccg
    18001 cgacgaccgc gacgccccgg tcggcgagga cgcgcagcag atccagcacc gcgagccgcg
    18061 cgtcgaggtc gagcccgagc gtgagctcgt cggccaggag cgcgaccggc gtgcgataca
    18121 gcgtctgcgc gaccgcgagc aggcgttgct cgccgcccga cagcacgccg gccttcgcgc
    18181 ggcggcggcc ctgcagcgtc ggcgcgagcg cgaggatcgc gtcggtcgcc tgctcgaacg
    18241 acttctgcgt ctgatgcgac cggtaggccg cgacacgcag gttctcgagc acggtcaggt
    18301 cgggaaagac cggccgggcg cccgacacga atgcaaggcc tgccgccgac cgctcctccg
    18361 gccgcagcgt cgtcacgtcg tcgccgtcga gcagaatccg tccggagccg ggcgtgacca
    18421 ggccggccgc cacgcgcagc agcgtcgact tgccggcgcc gttgccgccg acgagggcga
    18481 ccagctcgcc cgcgccgacc gtcatggtgg cctcgcgcag cacgtggttg gctccgaacc
    18541 gcaggtccat ccgctccatc accagcaccg gctcgcccga gcccggatgg cgcacgggct
    18601 cggcctgctt ggcggccatg gccgcgcgag cccggatgcg cttgcgatcc agatccagcg
    18661 aggtgcgtgc gcgaatcatg ttgatcgccg cacccgccgc gtaggcgatc gcccaccatc
    18721 ccgcgtcgcg ggcgccgccg ccggtgccgg cgcgcgccag gtgcagcccg ccgaagacgc
    18781 cggcgagcgc gaacgcgacc acgatgccgc tcgcggccgg cagcaccgac gggacgcacg
    18841 ctccggccgc cagccgcgcg gcctgaccgg ccgcccagcc cgacagagcg gggatcagcg
    18901 gcagcagcat cgcgacgagc acctcgtcgg ccgaggcgat cgccacgacc gacaggaaca
    18961 tcagcatcag caacgcgccg aagccgggcg ggatgcggta gggctgggcg gcgtcgccgg
    19021 gcagcgcggc cgcccccgtc tcggggccgc gccgccatgc gagcgtggcc gagatcaccc
    19081 cggagatgat cagcgcgacc gcgaaccacg gcgcgacgcg ctcgtccatg gccaggtgcg
    19141 cgtcccaggc cggcggcgcg acgatcgccg cggcccagcc gggcgccgac gcgaacgccg
    19201 cgccgaaggc cgcggcacgc accgtcgggg tggccatcgc ctcgcgcgta tcggaaccgg
    19261 cgacgcgcat cggggggctc atgggacgtt caccggatcg ggcgcctcgg ccggtgcggg
    19321 cgtcccgggc ggcgcgtctc gtcccgccgg gccggccagg gcccgcaccg cacggtcgcg
    19381 cagccattga aggccgcggc ccagcccgcc gggcaggaag gacacgacca gcaccacgcc
    19441 cgcgccggac gtgaccgcgg cccactcggg cggaaggaag atgcgcgccg cgcgaatcgc
    19501 gagcgcgccg agcagcgcgc cgcccggcga gccgagcccg ccgatcaccg ccatggcgag
    19561 caggtcgagc gaccagaccg gatcgaacgc gccgggctcg atgccggcgg gcgtggccgc
    19621 cagatacagc gaaccggcca ggccggccat ggcgcccgag atcgacaggg cgagcacgcg
    19681 gcttcgcatc acgttcacgc ccagccaggg tgcgcgctgc gaatcgtcgc gcgcagcgac
    19741 catgcgcatg ccggtcacgc tcgcgcgcaa ccggaccagc ccggcggcga gcgcgaccgt
    19801 cagaccgccg ccgatcatgc cgacggtgcg cacgtcggcg ggaaccgtgc tgtcgccgac
    19861 catcagcagc acgctcggcg cggatgccgc ggccgcaaga ccgaggaccg cgaacgacag
    19921 gctcgactgt cgccgcgtgg ccagcgccat gccggcgccg gtcgcgccca tcgcggcggc
    19981 cgccacgagc gcgcggacaa cccacggtcc cggcgtgagc gcggcgacgg cggcgccgag
    20041 gccggcgacg ccccaatgac cgagcacgac ctcgcccgcg aacatccatg cgacggcgac
    20101 ggcgagcgcc gcgatggcgt aggcggcggt ggtcgcgaag cgaacctggc tgctggccga
    20161 cagcaccagc ggtggcagcg cggcgaccgc gatcgcggcg acggcgaaga acgcgccgcc
    20221 gagctgcacg cccggcagcg acgccaggcg ccacggcacc ggacgcggcg cggccgcggc
    20281 ctcccacgac gaggcgcgtg cggccgcttc ggcgcggccc agccggcggc gctgcaacgc
    20341 caccgcgacg agcatcgcgg ccgcgagcga cagatcggcc acgcccgcgc gcggcgagta
    20401 ccacaggatc agctgatagc ccacgcccag cgcgagcgcg gcggccgcgg ttcccgcgat
    20461 cgagcggagc ccggcgaaca ccgcgggcgc gagcatcagc agcagcacgg tcgggccgag
    20521 gccgccgccg atattgaagc cgagcacggg gatcgaaagg atgccactca cgctcgcgag
    20581 cgcgccggcg acggcccaga ccgcgccgcg cacgacgccc gccggcacgc cgagcgtgcg
    20641 cgcgcgctcc tggtcctgcc cgagcgcgag cgcggccagg ccgatccggc tgcggtgcac
    20701 gaagtgcagc gttgcggcga gcgcgaccgg aaagacgatc aacgccagca aatgcggccc
    20761 gagcagcttg accgggaaca gctcgagcga gaatccgagg tcgatcgtgt aggtggggag
    20821 ccgtccgccg aagatgaacg gcagcgcggt ctggatcgcg gcgaacagcg acgccaggcc
    20881 caccgttgcg atcagcagca ccacgcgcgg ggcgtcctgc agccgccgca gcacgattcg
    20941 ctcggagccc agacccagca gcgcgccgac ggccaggccg agcggcgccg cccaccaaaa
    21001 gctccactgc gactgcgtga ccaaaagccc gaccagcacg ccgcccacga gaccgagcgt
    21061 ggcttgagcg aggttgatca cgcgcatggt tcgccagagc aggaccagcc cgaggctgtt
    21121 gagtcccgcc agcgagcctt ggacgaggcc gagtgcgatg atgcccgcgg gtggcacgtt
    21181 cgaaaggttc ctctccgctc ggagagcgcg cgttccggct agtagtccga cgcccgccgg
    21241 tagcgtttgc tctcaggata gtcgtatgcc ccggcgtgat tggagcccgg gtttcgtttg
    21301 gtcgggttcc aacgcagctt ggtggcgtcg ccgatctggc cgccctggga catatcgatc
    21361 gtgatcagca tcgagcgggg attggtggtg cgtccttcga tggcgtcgta gcggtagggc
    21421 ttgaacatgc tgagcgccac ccggaacgac tcaagcgtga ggtctcgtcc ggcgtcggtg
    21481 atgccggcaa ggatcgggat gagcgactgc gcgacgaacg ccgtggggga gggcggctcg
    21541 gtgttcggcc ggacttcctt gaacatcttc caccactcgg tctgcttggc gatcccgtcg
    21601 ttggtcaggt ccgggttgaa gtgctcgtcg tagcaggtca gcccgaacgc gcgcgtccat
    21661 tgggttgcgt tgtagaggcg aaccatggcg gcgttgtcct gccccgagca aggccacacg
    21721 aggtagtcgg ggctgtagcc gttggcctgc gccgcgtggg tgaagttcag cggcgctccg
    21781 gcgtccggga tgatgagcgt gttgacgcct gcgtcccgga acttgttcgc aagcgtcgac
    21841 gccatggtct gtccggtggt cagatcggcc gcgatcgaca gcgacaacgc gatgtcgata
    21901 ccggccttct cgatcatccg ctcgaacttc ggggccgcgt ccaccagccc gggatactcg
    21961 gtgtagagca agccgtagac gcgcttctgt ccgacgttcg cggtcatgta ctgggtcttg
    22021 ttcacctgct tggtcacgta ggcgatcatc gcctcgacct gatcggacca cgactgccct
    22081 gccgacatgc agtaaccgtt cgtcttttgg tacagccccc agttcaggtc ataggtgctc
    22141 gggttgaaga tgcccgcctt ggcgagacgc ggacagatgt aggcgctgag cgagccgtgc
    22201 gaggagatcg cggcgaaggg ccccggcttg gccgcgccct tgagcttgag gtcgcgcgcg
    22261 agaaactcgc ccgtggccgc gtagtcgtcg ggatactgtc cggcctcttt cacgaagcct
    22321 ttcagcttgc ggccgtgcag gttgaacttg tagccgagaa actcgctgcc gtcggtggcg
    22381 tgcttgttga tccagtcgat catcgccata aaggcgacgc cttcgtcgac gttgccctct
    22441 tgcccggtac cggtctggtt caggtacggg ctggttttgg tccggtcgcc cttccagtag
    22501 taggcgagca agatctcgtt gtcgctcacg cccggcagcg ccgttcgcgt cacgccgggc
    22561 gtgagcgaca acgagatctt gctcgcctac tactggaagg gcgaccggac caaaaccagc
    22621 ccgtacctga accagaccg
//
LOCUS       DATLFO010000275         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_88920, whole genome shotgun sequence.
ACCESSION   DATLFO010000275 DATLFO010000000
VERSION     DATLFO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88920"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2364
                     /locus_tag="VM841_11260"
     CDS             <1..>2364
                     /locus_tag="VM841_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90798.1"
                     /translation="VQLTSATLLTDFNREIRANAGDQVEIDYFDARLATAGSQRTVTL
                     AMPVRAPSAGGHGPFDGFVGGAGVPQADFFGRQDYANLTITDNDRNVDPTAIDTLRAT
                     LEVFAISSAGQASFPLKTVPVTLFETGPDTDVFAARLKFGPYVGIPPKGLTVLDLGTA
                     AGTQYVVHGLYEDRARPACTADVLPGCMPTGAHQPSTFDSRIVMTARYRPATPGSLSV
                     TPTTLEGLGQILTVRVSDSDRDGRVLQERVGLGNGVGNHQVRTVNVPASGSQIVYVET
                     APGSKVFRAWRHVNAFTIGQALEEYRIDANGVITFGDCQIAGSACTVPTTGGKIPLAG
                     VEIRADYRQQNVRNDVYVRLESYLVDPDQPFDPARDARPMAVACGTSANPNCHDRLDL
                     RILELTTTAGQFDANVPLAIGAAALNDLALQLPPGDPTRVDRSVIVVTYFDYAYIENL
                     RPRANAGTFQQLDCSPNHPADVDNEPGAPFSQVMPGQPAVDGDCQFPFTVRDLVQATV
                     AFTESTTAQLAVHSGNQRLANGVLDAAKGPRAVFGTQPLGIRVADADLNRDPLKSELA
                     TVVLKTDSNPLGVEVSLAETSANSGVFAARVRLGPGGVIAANHGDRYTVSYTDQHRES
                     GGSGVVSLPAVPEDATDYRWFQASSGAVSLTRSVYIGTDMPASVSGYSVPRIRAVDMD
                     RNRNPAALDQVKVRYAHAALTGNVLTEAGARALYAVDNCRTPAASGNEATLTETGVST
                     GVFEGTFPYPAANKPLHGETLLFTYCDPHDEAGREGPARSGPLARIG"
BASE COUNT          351 a          888 c          806 g          319 t
ORIGIN      
        1 cgttcagttg acgtcggcga cgctgctgac cgacttcaac cgcgagatcc gcgcgaacgc
       61 cggcgaccag gtcgagatcg actacttcga cgcgcgcttg gcgacggccg gctcgcagcg
      121 cacggtgacg ctcgcgatgc cggtccgcgc cccctccgcg ggcggccacg gccccttcga
      181 cggtttcgtc ggcggcgccg gcgtgccgca ggccgacttc ttcggccggc aggactacgc
      241 gaacctgacg atcacggaca acgaccggaa cgtcgacccg acggcgatcg acacgctgcg
      301 ggcgacgctc gaggtgttcg ccatctcgtc ggccggccag gccagcttcc ccctgaagac
      361 ggtcccggtg acgctattcg agacggggcc cgacacggac gtgttcgcgg cccgcctcaa
      421 gttcggtccc tacgtcggca tcccgccgaa gggcctcacc gtgctggacc tcggcaccgc
      481 cgcgggcacg cagtacgtcg tgcacggcct ctacgaggac cgtgcgaggc ccgcttgcac
      541 cgcggacgtg ctgccgggct gcatgccgac cggcgcgcac cagccctcga cgttcgacag
      601 ccgcatcgtc atgacggccc gctaccggcc cgccacgccc ggttcgcttt cggtgacgcc
      661 gacgaccctg gagggtctcg gccagatcct cacggtccgt gtctccgact cggaccgcga
      721 cggccgcgtc ctgcaggagc gcgtcggcct gggcaacggc gtcggcaacc accaagttcg
      781 cacggtgaac gtgcccgcgt ccgggtcgca gatcgtctac gtggagacgg cccccggctc
      841 gaaggtgttc cgcgcgtggc gccacgtcaa cgccttcacg atcggccagg cgctggagga
      901 gtaccgcatc gacgcgaacg gcgtcatcac gttcggcgac tgccagattg ccggcagcgc
      961 gtgcacggtc ccgaccacgg gcggaaagat cccgctcgcg ggcgtggaga ttcgcgccga
     1021 ctaccggcag cagaacgtcc ggaacgacgt gtacgtccgc ctcgagtcct acctggtgga
     1081 cccggaccag ccgttcgacc cggcgcgcga cgcgcgcccg atggcggtgg cgtgcggtac
     1141 ctccgcgaac ccgaactgcc acgaccggct cgacctgcgc atcctcgagc tcacgacgac
     1201 cgcgggccag ttcgacgcga acgtgcctct cgcgatcggc gccgccgcgc tgaacgacct
     1261 ggccttgcag ctgccgccgg gcgacccgac gcgcgtggac cggtccgtca tcgtggtcac
     1321 gtacttcgac tacgcgtaca tcgagaacct gcggccgcgg gcgaacgcgg gcacgttcca
     1381 gcagctcgac tgctcgccga accaccccgc ggacgtggac aacgagccgg gcgcgccgtt
     1441 cagccaggtg atgccgggcc agccggcggt ggacggcgac tgccagttcc cgttcacggt
     1501 gcgcgacctc gtccaggcga cggtggcgtt caccgagtcg acgacggcgc agctggcggt
     1561 gcactcgggc aaccagcgtc tcgcgaacgg cgtcctggac gcggcgaagg gcccgcgcgc
     1621 ggtcttcggc acgcagccgc tcggcatccg cgtcgcggat gcggacctca accgcgatcc
     1681 gctcaagtcg gagctcgcca cggtcgtcct gaagaccgat tcgaacccgc tcggggtcga
     1741 ggtctcgctc gcggagacca gcgcgaacag cggcgtcttc gcggcgcgcg tgcgcctcgg
     1801 tccgggcggc gtcatcgccg cgaaccacgg cgaccgctac acggtcagct acacggacca
     1861 gcaccgcgaa tcgggcggtt ccggcgtcgt ctcgctgccg gcggtgccgg aggatgcgac
     1921 ggactaccgc tggttccagg ccagctcggg cgccgtctcc ctgacgcgct cggtctacat
     1981 cggcacggac atgccggcct cggtgagcgg atacagcgtc ccgcgcatcc gcgccgtgga
     2041 catggaccgc aaccgcaacc cggcggcgct cgaccaggtg aaggtgcgct acgcgcacgc
     2101 ggccttgacg ggcaacgtgc tcacggaagc gggcgcccgt gcgctctacg ccgtggacaa
     2161 ctgccgcacg ccggcggcct cggggaacga ggcaacgctc acggagacgg gcgtctcgac
     2221 cggcgtcttc gagggcacct tcccctaccc ggcggccaac aagccgctgc acggcgagac
     2281 cctgctcttc acctactgcg acccgcacga cgaggcgggc cgcgagggcc ccgcgcgctc
     2341 cgggccgctc gcacgcatcg gctt
//
LOCUS       DATLFO010000276        12469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_135529, whole genome shotgun sequence.
ACCESSION   DATLFO010000276 DATLFO010000000
VERSION     DATLFO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135529"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VM841_11265"
     CDS             complement(<1..615)
                     /locus_tag="VM841_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVL90799.1"
                     /translation="MAAPAIGIDVGGTKIAAAVVDETGGVIARDVVATDADAPNAIAT
                     GIVKIARELRAVAPSVGAIGIGAAGLVDTARGLIIAAPNLSYRNLAIRDYVQTRLSLP
                     VFVDNDANVAAWGEARFGAGRGRGDQVMITVGTGIGGGIILDGRVYRGAHGFGAEIGH
                     MIVQPGGPACPCGANGCYEQLASGNAIGRVASERIGEAPGSLVLE"
     gene            complement(622..1755)
                     /locus_tag="VM841_11270"
     CDS             complement(622..1755)
                     /locus_tag="VM841_11270"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HVL90800.1"
                     /translation="MADRHEPGGIPTEQSLRALASMDAGDAAVVSFYLDTDGRRSPRR
                     SDLERATADALRAVESQDLSRAARASVTADCARIREYVAKEFQRNSTRGLAVFASDAI
                     DLWNVCHLPAPVRTRAVADRHPHVLRLEALVSRAERFATVLISRDKARLFTTFLGHTE
                     ERSGVLDDVPGKHGQGGWSQANYSRHIDEIAHRHSKHAAEVLFELSRKEPFDRLILAG
                     PEEAAGTFEKDLHPWLAERVAGRISLPMTAGIARVAEAVREAEDRIEDERAAEAVARI
                     LEEFAAGRSAVVGLGKTLAALGEGRVETLAIADGEPRAGYRCDVCARLAATDGACGAC
                     GGAMLGVADLNEEIVDEALRKRCGVVAAQTRPLPDGIGALLRF"
     gene            complement(1800..2069)
                     /locus_tag="VM841_11275"
     CDS             complement(1800..2069)
                     /locus_tag="VM841_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90801.1"
                     /translation="MNEEPKGGSGGGLFGGIPQPNWGQTQDCMLCPFGLVAFAVRNTN
                     PEMMEHLVKAGMELMLAFKALADAAADKLGHTSRPEDGLQRIDIS"
     gene            complement(2066..3385)
                     /locus_tag="VM841_11280"
     CDS             complement(2066..3385)
                     /locus_tag="VM841_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HVL90802.1"
                     /translation="MRILLFTGKGGVGKTTVSAATAVHAARRGLRTLVMSTDPAHSLA
                     DAMAEPLDGEPREVAPNLWGQQIDAQKRLEEGWREIQEYVLTLLDWGGVDAIAAEELS
                     VIPGLDEIFALTDVRRAADSMAYDLLVVDCAPTAETLRLLSLPDALNWYFERIFPMER
                     RIAKVARPVVNRMVKAPIIASDTVMGAVERLHRTLESVRRLLIDTETSSVRLVVNAER
                     MVIAEARRTYTYLNLFGYRVDAVVCNRLLPETITDPYFEEWKALQKMHLGTIHESFAP
                     VPIMNVPLYDREMVGLDRLGVLAENLYGETDPAAMLHDVDPVVVRKTAEGATMSLHLP
                     FTEKADIDLLRKGDDLYVKVGPYKRAIMLPVSLSRLEVAGAAFEGEALVVRFKRPPGE
                     EPEPEPAEPAKRRRGRGAASGAHGEAAKKSAARPRPARAAEAEGSDR"
     gene            complement(3443..3886)
                     /locus_tag="VM841_11285"
     CDS             complement(3443..3886)
                     /locus_tag="VM841_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL90803.1"
                     /translation="MADQTQGSIVIEADPAVIMAEIADYDSYPEWSGEIKKVEIRETD
                     DQGRGKRVSFEVASGPLKADYVLDYRYKPGDAGVSWTFVEGTNLRNMEGEYTLAAQAP
                     GKTNVTYTLKVDTPIPMLGFMKRQVEKKIIDVALKGLKKRVESRS"
     gene            complement(3960..4661)
                     /locus_tag="VM841_11290"
     CDS             complement(3960..4661)
                     /locus_tag="VM841_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90804.1"
                     /translation="MSEPVRCPACGASNAAGASWCGQCLTRFDSAAAGAAEAGPAASA
                     AAPAAAAAGPAVPAPAPLRSVASEGVHRRGEDLVWTCPTCAAVNEMSHTACPVCATPI
                     AVLFGATKRGSGPARTGPMVIVLSAVLPGAGHGYAGRAADAIARGVLFVWTTAIGILL
                     LTRTTARGTGVFRAIGAVFVITAAGTWLITMLEAQRLARGDDDPLVPGKVLLWASAAL
                     TGLLFVGLAAGARTR"
     gene            complement(4658..5074)
                     /locus_tag="VM841_11295"
     CDS             complement(4658..5074)
                     /locus_tag="VM841_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL90805.1"
                     /translation="MTVNTTDEIFVDAEPADVYRALLTFGKDASWWPGAKSESSGKRI
                     TVTLPVGALTRVRFSATVDNVRPDEGLTWVFEDGELTGRGEWWLEPFKDGTIVHYYLI
                     EAGKAGGTRGLDALVDKHRVAVRRGMNALKDFMEGR"
     gene            5188..5655
                     /locus_tag="VM841_11300"
     CDS             5188..5655
                     /locus_tag="VM841_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HVL90806.1"
                     /translation="MAERDRDEQGRAKNARPRDALGRPLPREAAAEYVEEPDPQSEDE
                     ALDRGIEHFNNGRFFQAHEMWEFAWHPSPEPDRDFWQGITQVAVGFTHFQRGNVNGAI
                     TLLDRGAAKLEKYGKVHKTLPVAEIASAARAAQHEIERSGTEASPVPPEIRRV"
     gene            complement(5663..6199)
                     /locus_tag="VM841_11305"
     CDS             complement(5663..6199)
                     /locus_tag="VM841_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90807.1"
                     /translation="MKKRLMAFGIAGAAFLAPAGALAGHGTGCDYTASQEHSTTVGSV
                     VIYADTDGGGGTSGTADQAAGVCAGPGSFEAGHSATLGSDRSAWYGLGIHNGLYLIAD
                     GDNLENPDPVDGYIGISNYETGSTSCRNNTDCSGSNGGGEVGPGGGPYAPVYVPLACG
                     NTTGPSWDDSRRDGCEIP"
     gene            complement(6316..7857)
                     /locus_tag="VM841_11310"
     CDS             complement(6316..7857)
                     /locus_tag="VM841_11310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HVL90808.1"
                     /translation="MSRPPVPFDVSEAARGGIPERILRGVRSSEEAYEILRRPVTRRS
                     VLKGAGAAALAGPVLWTRPGRASEPPRGIHLAYGDDPGTSMSVSWFTGGPVAGAALEI
                     GDTPAFGTTIPAASTTYSPPPSIPQPISRQHHATATGLEPGTRYHYRVRHDGAVSPAF
                     TFETAPGAIVPFRFTSFGDHGTDPGTATSRVSWGTAFSPRAVRAVTALDPAFHVHNGD
                     LSYSCGGPQLAWDVWFEQIQSRAAVMPWMVALGNHEMELGFGPRGYDPFRSRFRLPAN
                     GIDSGPLRTSTFYAFQYSNVLVMMLDGNEAASEYGYHFNRGYLAGAQDAWIEQTLQAA
                     RTSPTIDWVVVSFHNCMFCTDVVHGSDGGCRTRWQSLFDAYRVDVVLNGHNHCYERTH
                     PIRGHGHTTLMPGEPVEPATMGVTYVCAGGGGQLAAPRSVSTYPASLLHNEAGEREIE
                     HALWRASRIEGYTVAVVDVDPGAPGGTTTLTLTAREFMTDLMAPVDRVVLTRPAKAVA
                     AVRGR"
     gene            complement(7901..8833)
                     /locus_tag="VM841_11315"
     CDS             complement(7901..8833)
                     /locus_tag="VM841_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HVL90809.1"
                     /translation="MANETGMKLFVRATRAKVLPVMLTPVTVGAAVGWERSGVWSWPR
                     FALTVAGAAAMHLAANVINDIYDEESGAEVRARADHRSVATGSGVLGAGLMSKRQLLG
                     LAGALFSVALACAGVLAALGRPHVLWLGVIGFVLAWEYVGPPLRYGFVGRGLGEVGIF
                     LAFGLLPVAGSAYVQAGRFDAAALWASVVPGLLAMLVLYHHHFLHWQSDRSVGKMTPV
                     AALKPDIALVVGRIALIATTIALAIQTLALSLFPPAAFLAALSFAPVAFAQQGAAEDP
                     GNEAYVRVLGASLGASVVTGAVLVLAAAVRVALR"
     gene            8941..10206
                     /locus_tag="VM841_11320"
     CDS             8941..10206
                     /locus_tag="VM841_11320"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90810.1"
                     /translation="MTIETPIPSGGLLRRNPGFARLYLSAIVSFAGDWFAVVALQGLV
                     LETTGKPALAGLMLATSTLPFAIVSPFAGVIADRVDRRKLMIGADLARAAVALGFLFA
                     RDASTIWIAFVCTALLSAFAPFFEPASSASLPNLVRRQDLARANVLMGSAWGTMLAVG
                     AALGGLVAATLGREAAFLMDSGSFLVSALLLRSIREPFQEKIPEPTQVTIVQDIAVAL
                     RFVREEARVRAILVAKGLFGSAGGTIALLSTMAVQVFERGDTGIGILMGARGVGALAG
                     PFLFRRFLLRNKDERLLVSIAFAFAVFSICYALFAGAPSLWLAAAAVCGAHIGGGANW
                     TISAYALQRFTPDHVRGRIFAFDYSLITMTMTTSLSLAGFAAGIFDVRAVAFGFAMIG
                     MVMMLGWTAWAWRLIGPRALRRAREATAT"
     gene            10252..12276
                     /locus_tag="VM841_11325"
     CDS             10252..12276
                     /locus_tag="VM841_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVL90811.1"
                     /translation="MPNRLASASSPYLLQHAGNPVDWYEWGPEALQRAKDEDKPIFLS
                     IGYAACHWCHVMERESFEDPEIAAFMNDHFVNVKVDREERPDLDQIYMNAVQAMTGHG
                     GWPMTMFLTPDGAPFYGGTYFPPEDRQGLPAFRRVLMGVAEAWRSQRDEARTQSINLL
                     AHITKMTEGLPAGGALDPSILRTAFDAMAANFDQQWGGFGGAPKFPQPMTLEFLLRAS
                     RLGFAGARDMLTLTLTRMARGGIFDQVGGGFHRYSVDGFWLVPHFEKMLYDNGQLLRL
                     YARAAVALDDPHRLFAGTAIATGEYLLREMRHPGGGFYSSGDADSEGEEGKFFVWTTD
                     EIRRIAGEDDEIVSAAWSLQHGGNFEGKTIPWMPLPLEEVAHATGAKVERIRAALDRV
                     KGEMFALREQRVRPAIDDKILASWNGLAIAGLCEAGRLLDRDDFIVAAMQAADFALAN
                     LRRDDGRLLRSWRDGRTSGPGYLDDYALLAGALLDVYETTFQTRYFTEARALADAMIE
                     LFAAEDGGFYDTGSDAEALVLRPRDVFDNAVPSGSSSAASLLLRLAALTGEASYEHRA
                     IASLQRVTGIAAQAPLGFGEALCAMDSATAGIKEIAITGTGPETRWLADVVWRRVALP
                     RVLAGGDEETVPLMKGRTERNGVATAYVCERFACKEPVTDRDALAAQLDG"
BASE COUNT         1815 a         4465 c         4353 g         1836 t
ORIGIN      
        1 ctcgagcacc agcgagccgg gcgcttcgcc gatgcgctcg ctcgcgacac ggccgatcgc
       61 gttgccggag gcgagctgct cgtagcatcc gttcgccccg cagggacagg ccggcccgcc
      121 gggttggacg atcatgtggc cgatctcggc gccgaagccg tgcgcgccgc ggtagacgcg
      181 cccgtccagg atgatgccgc cgccgatgcc ggtcccgacc gtgatcatga cttgatcgcc
      241 gcggccgcgg ccggccccga agcgcgcctc gccccacgcg gcgacgttcg cgtcgttgtc
      301 cacgaacacc ggcagcgaca gccgcgtctg cacgtagtcg cggatcgcaa ggttgcggta
      361 cgaaaggttc ggcgcggcga tgatcaggcc gcgggcggtg tcgaccaggc ctgcggcgcc
      421 gatgccgatc gcgccgaccg acggcgcgac cgcgcggagc tcgcgggcga ttttgacgat
      481 gccggtcgcg atggcgttgg gcgcgtcggc gtcggtcgcg acgacgtcgc gggcgatcac
      541 gccgccggtc tcgtccacca ccgccgcggc gatcttggtg ccgccgacat cgatgccgat
      601 cgcgggcgcg gccacggcgc cctagaagcg cagcagcgcg ccgatgccgt cgggcagcgg
      661 ccgggtctgg gcggcgacga cgccgcaacg cttgcgcaag gcttcgtcga cgatctcctc
      721 gttgaggtcc gccacgccga gcatcgcgcc gccacacgcg ccgcatgcgc cgtcggtagc
      781 ggcgaggcgc gcgcacacat cgcatcggta gccggctcgc ggctcgccgt cggcgatcgc
      841 cagcgtttcc acgcggccct cgcccagcgc cgccagggtc ttgcccaggc ctaccacggc
      901 ggagcgcccg gcggcgaact cctccagaat gcgcgccacc gcctcggccg cgcgttcgtc
      961 ctcgattcgg tcctcggcct cgcgaaccgc ctcggccacc cgggcgatgc ccgcggtcat
     1021 cggcagggag atccgtcccg cgacccgctc ggcgagccac ggatgcaagt ccttctcgaa
     1081 ggtgccggcg gcttcctcgg gcccggcgag gatcagccgg tcgaacggct ccttgcgcga
     1141 gagctcgaac aggacctcgg ccgcgtgctt ggagtgccgg tgcgcgatct cgtcgatgtg
     1201 ccgcgagtag ttcgcctgcg accagccgcc ctgcccgtgc ttgccgggca cgtcgtcgag
     1261 cacgccgctg cgttcctcgg tgtgaccgag gaacgtcgtg aaaagccggg ccttgtcccg
     1321 gctgatcagc acggtggcga accgctcggc gcgcgagacg agcgcctcga gccgcagcac
     1381 gtgcggatgg cggtcggcga cggcgcgtgt gcgcaccggc gccgggaggt ggcacacgtt
     1441 ccacagatcg atcgcatcgg aggcgaagac ggcgaggccg cgcgtcgagt tccgctgaaa
     1501 ctccttggcg acgtactcgc ggatgcgcgc gcaatcggcc gtgaccgagg cgcgcgcggc
     1561 gcgcgacagg tcttgggact cgacggcgcg cagcgcgtcg gcggtggcgc gttcgaggtc
     1621 ggatcgccgg ggagaccggc gcccgtcggt gtcgaggtag aacgacacga ccgccgcatc
     1681 gcccgcgtcc atggaagcga gcgcgcgcag ggactgttcg gtcgggatgc cgccgggttc
     1741 gtgccgatca gccatcgccg cgagtgtacc cgcccgcgcg cccgcgaatc cagaccgggt
     1801 caggagatgt cgatgcgctg caggccgtcc tcgggtcggc tcgtgtgccc gagcttgtcg
     1861 gccgccgcgt cggctagggc cttgaacgcg agcatcagct ccataccggc cttgaccaga
     1921 tgctccatca tctcgggatt ggtgtttcga accgcgaacg caaccaagcc gaacgggcac
     1981 agcatgcaat cctgcgtctg gccccaattc ggctgcggga tgccgccgaa gagacccccg
     2041 ccggatccgc ccttgggctc ctcgttcacc gatcgctccc ctcggcctcg gcggcgcgcg
     2101 ctggacgcgg ccgcgctgcg gacttcttcg cggcctcgcc gtgcgcgccg gacgcggcgc
     2161 cgcgcccccg gcgccgcttc gccggctcgg cgggctcggg ttccggctcc tcgccgggcg
     2221 gccgcttgaa tcgtacgacg agcgcctcgc cctcgaaggc cgcgccggcg acctcgaggc
     2281 gcgacagcga cacggggagc atgatggcgc gcttgtacgg cccgaccttg acgtagaggt
     2341 cgtcgccctt gcgcagcaga tcgatgtcgg ccttctcggt gaacggcagg tgcagcgaca
     2401 tcgtcgcgcc ctctgcggtc ttgcggacca cgaccggatc gacgtcgtgg agcatggccg
     2461 ccgggtcggt ctcgccgtag aggttctcgg cgagcacgcc gagccggtcc agcccgacca
     2521 tctcgcggtc gtagagcggc acgttcatga tcggcacggg tgcgaacgat tcgtggatgg
     2581 tcccgagatg catcttctgc agcgctttcc actcctcgaa atacggatcg gtgatcgtct
     2641 cggggagcag ccggttgcac acgaccgcgt cgacgcgata gccgaacagg ttcagatacg
     2701 tgtaggtgcg gcgcgcctcg gcgatgacca ttcgttcggc gttgacgacc agccgcaccg
     2761 acgaggtctc cgtgtcgatc aggagccggc gcacggactc gagcgtgcga tgcagccgtt
     2821 cgaccgctcc catcacggtg tccgaggcga tgatcggggc cttgaccatg cggttgacca
     2881 cggggcgcgc taccttggcg atgcggcgct ccatcgggaa gatgcgctcg aagtaccagt
     2941 tgagcgcgtc gggcaagctc agcaggcgaa gcgtctcggc cgtcggggcg caatcgacca
     3001 ccaacaggtc gtaggccatg ctgtcggcgg cacgccgcac gtcggtgagc gcgaagatct
     3061 cgtcgaggcc cgggatcacc gaaagctcct ccgcggcgat ggcatccacc ccgccccagt
     3121 ccagcagcgt cagcacgtac tcctggatct cgcgccagcc ttcctcgagc cgcttctgcg
     3181 catcgatctg ctggccccat aggttcgggg cgacctcgcg cggctcaccg tcgagcggct
     3241 cggccatcgc gtcggcgagc gagtgcgccg gatcggttga catcacgagc gtgcgcaggc
     3301 cgcgtcgcgc ggcgtgcacc gcggtcgcgg cggaaacggt cgtcttgccg accccgcctt
     3361 tgccggtaaa gaggaggatt cgcatggagc ggccagcata ccgggggcgc gggaccgccg
     3421 ctttcgaacg tgaaacgagc ttttaggagc gtgattcgac gcgctttttc agccctttga
     3481 gcgccacgtc gatgatcttt ttctcgacct gacgcttcat gaagccgagc atcgggatcg
     3541 gcgtgtcgac cttgagcgtg taggtgacgt tcgtcttccc gggcgcttgc gccgcaagcg
     3601 tgtactcgcc ttccatgttg cgaaggttcg tgccttcgac gaaggtccac gacacgcctg
     3661 cgtccccggg cttgtaccgg tagtccagca cgtagtcggc cttgagaggg ccgctggcga
     3721 cttcgaaaga aacccttttg ccgcggccct ggtcgtcggt ctcgcggatc tcgaccttct
     3781 tgatctcccc ggaccactcc ggataggaat cgtagtctgc gatctccgcc atgatcacgg
     3841 ccggatccgc ctcgatcacg atcgagcctt gcgtctggtc cgccatctgc cctcccgccg
     3901 ccggatgtgc ggaaatccta gcgtggcgcc tgtgcccgtg aaaggagcga gccgcgcctt
     3961 cagcgcgtgc gcgcgcccgc agcgagcccg acgaacagca ggccggtcag cgccgccgac
     4021 gcccacagca ggaccttgcc gggaacgagc ggatcgtcgt cgccgcgcgc gagccgctgc
     4081 gcttcgagca tcgtgatcag ccacgtgccc gccgcggtga tgacgaacac cgcgccgatc
     4141 gcacggaaca cgcccgtgcc gcgcgccgtc gtccgtgtca gcagcaagat cccgatcgcc
     4201 gtcgtccaca cgaacagcac gccgcgcgcg atcgcgtcgg cggcccgccc ggcatagcca
     4261 tggcccgcgc ccggaagcac agcggagagc acgatgacca tcggcccggt gcgcgcgggc
     4321 ccgctccccc gcttcgtcgc gccgaacagc accgcgatcg gcgtcgcgca gacgggacac
     4381 gccgtgtgcg acatctcgtt gacggccgcg caggtcgggc aggtccacac cagatcctcg
     4441 ccccggcggt gcacgccttc ggacgcgacg cttcgcagcg gagccggcgc gggtaccgcc
     4501 gggccggcag cggcagccgc cggggccgca gcgctcgcgg cgggtccggc ttcggccgcg
     4561 cccgcggcag ccgaatcgaa ccgggtcagg cattgtccgc accacgacgc gccggccgcg
     4621 ttgctcgccc cgcacgcggg gcagcgaacc ggttcgctca tcgcccctcc atgaagtcct
     4681 tcagcgcgtt catcccgcga cgcaccgcca cccgatgctt gtccacgagc gcgtcgagcc
     4741 ctcgcgtgcc gccggccttg ccggcctcga tcaggtagta gtggacgatg gtgccgtcct
     4801 tgaacggctc gagccaccac tcgccgcggc cggtcagctc cccgtcctcg aacacccagg
     4861 tgaggccctc gtccggacgc acgttgtcca ctgtggccga gaagcgcacg cgcgtgagcg
     4921 cgccgaccgg cagggtgacc gtgatgcgct tgccggacga ctcggacttg gcgcccggcc
     4981 accacgacgc gtccttgccg aacgtcagca atgcgcgata gacgtcggcg ggctcggcgt
     5041 cgacgaagat ctcgtccgtc gtgttgaccg tcacgcgccc gagtataggc gtggctcgtg
     5101 cggggttcgc gcagccacga tgggcccgaa accacgatcc ggccggcggt gcggcagccc
     5161 catcgtggtc gcggctagca tgtacccatg gcagagcgcg accgtgacga acagggacga
     5221 gcgaagaacg cgcgtccgcg cgacgccctc ggccgcccgc tgccgcgcga ggcggctgcc
     5281 gagtacgtcg aggagcccga tccgcagagc gaggacgagg cgctcgaccg cggcatcgag
     5341 catttcaaca acggccgctt cttccaggcg cacgagatgt gggagttcgc ctggcatccc
     5401 tcgcccgagc cggaccgcga cttttggcaa ggcatcacgc aggtggcggt cggcttcacg
     5461 cacttccaac gcggcaacgt caatggcgcg atcaccctgc tcgatcgcgg cgcggccaag
     5521 ctcgagaagt acggcaaggt ccacaagacg cttcccgtcg ccgagatcgc atccgccgcg
     5581 cgcgcggcgc agcacgagat cgaacgaagc gggacggagg ccagccccgt cccgcccgag
     5641 attcgccgcg tctagagagc ccctacggaa tctcgcagcc gtcgcgacgc gagtcgtccc
     5701 acgacggacc ggtcgtgttg ccgcacgcga gcggcacata caccggcgcg tagggaccgc
     5761 cgcccggacc gacctcgccg ccgccgttgc tgccgctgca gtcggtgttg ttccggcagc
     5821 tcgtgcttcc ggtctcgtag ttggagatcc cgatgtagcc gtccaccgga tccgggttct
     5881 ccaggttgtc gccgtcggcg atgaggtaga gcccgttgtg gatgccgagg ccgtaccagg
     5941 ccgagcggtc ggatccgagg gtcgcgctgt ggcccgcctc gaacgagccc gggccggcgc
     6001 acacgcccgc ggcctgatcg gcggtcccgc tggttccgcc cccgccgtcg gtgtcggcgt
     6061 agatcaccac gcttcccacg gtcgtcgagt gctcctgcga ggcggtgtag tcgcatccgg
     6121 tgccgtggcc cgcgagcgcg ccggccggcg cgaggaatgc ggcgccggcg atgccgaacg
     6181 ccatcagacg cttcttcatg gatcctcccg atcgattcgc cgccaggccg caccggcctc
     6241 gcgcccctcg ccccgttgcc cggttcgatg cctacttcga tgcgcgcccc gcgggctcct
     6301 tccgcgggac cccgctcagc ggccgcggac cgctgccacc gccttcgccg gccgggtcag
     6361 cacgacgcgg tccaccggcg ccatcaggtc ggtcatgaac tcccgcgcgg tcagcgtgag
     6421 cgtggtcgtg ccgcccggcg cgcccgggtc cacgtccacc accgcgaccg tgtagccctc
     6481 gatgcgcgag gcgcgccaca gcgcatgctc gatctcgcgc tccccagcct cgttgtgcag
     6541 caggctcgcc gggtaggtcg agaccgaccg cggcgcggcg agctggccgc cgccgcccgc
     6601 gcacacgtag gtgacgccca tcgtcgcggg ctcgaccggc tcgcccggca tcagcgtggt
     6661 gtggccgtga ccgcggatgg gatgggtgcg ctcgtagcag tggttgtgcc cgttgagcac
     6721 gacgtcgacg cgataggcat cgaacaggga ttgccagcga gttcggcatc cgccgtcgga
     6781 gccgtgcacg acgtcggtgc aaaacatgca gttgtgaaac gagaccacga cccagtcgat
     6841 cgtcggcgac gtccgcgcgg cctgcaaggt ctgctcgatc cacgcgtcct gcgcgccggc
     6901 gagatagccg cggttgaagt ggtagccgta ctcgctggcg gcctcgttgc cgtcgagcat
     6961 catcaccagg acgttggagt attggaaggc gtagaaggtg ctcgtccgca gcggccctga
     7021 atcgatgccg ttggccggaa gccggaagcg cgaccggaag gggtcgtagc cgcgcggacc
     7081 gaagccgagc tccatctcgt ggttgccgag cgcgaccatc cagggcatga ccgccgcgcg
     7141 cgactggatc tgctcgaacc acacgtccca agcgagctgg ggaccgccgc acgagtacga
     7201 caggtcgccg ttgtgcacgt ggaaggcggg atcgagcgcc gtgaccgcgc gcacggcccg
     7261 cggcgagaac gcggttcccc acgacacgcg gctcgtggcc gttcccggat ccgttccgtg
     7321 atcgccgaag gacgtgaagc gaaacggcac gatcgcgccg ggcgcggtct cgaaggtgaa
     7381 ggccgggctg accgccccgt cgtgccgcac gcgatagtgg taacgcgtgc ccggctcgag
     7441 cccggtcgcg gtcgcgtgat gctggcggct gatcggctgc gggatcgacg gcggcggcga
     7501 gtacgtagtc gatgccgccg ggatcgtggt gccgaacgcg ggggtgtccc cgatctcgag
     7561 cgcggcgccc gcgaccggcc cgcccgtgaa ccaggacacg ctcatcgagg tgcccgggtc
     7621 gtctccgtag gccaggtgga tgccgcgcgg cggctcgctc gcgcggcccg gacgcgtcca
     7681 caggaccggc cccgcgaggg ccgcggcccc cgcgcccttg agcacgctgc gacgggtcac
     7741 cggacgacga aggatctcgt aggcctcctc ggacgaacgc acgccgcgaa ggattcgctc
     7801 gggaatcccg ccccgggcgg cttcggagac atcgaaggga acgggtggac ggctcatcgt
     7861 ggtccgattc gccgcggccc ggccgggatc ctgcccggcg tcagcgcagc gcgacgcgaa
     7921 ccgcggccgc gagcaccagc acggcgccgg tcacgaccga cgcgcccagg ctcgcgccga
     7981 gcacccgcac gtaggcttcg ttgcccgggt cctcggccgc gccctgctgc gcgaaggcga
     8041 ccggagcgaa cgacagcgcc gcgaggaacg cggccggcgg gaacagcgac agcgccagcg
     8101 tctggatcgc gagcgcaatg gtggtggcga tcaaggcgat gcgtccgacg acgagcgcga
     8161 tatcgggctt gagggccgcg accggcgtca tctttccgac cgagcgatcc gactgccaat
     8221 gaaggaaatg atggtggtag agaacgagca tggccagcag cccgggaacg accgacgccc
     8281 acagggcggc cgcgtcgaag cggcccgcct gcacgtaggc ggagcccgcg acgggcagca
     8341 ggccgaaggc gaggaaaatc cccacctcgc cgaggccgcg gccgacgaac ccgtagcgca
     8401 gcggcgggcc cacgtactcc cacgcgagca cgaacccgat cacgccgagc cacagcacgt
     8461 gcggccggcc gagcgccgcc agcacccccg cgcacgcgag cgccacgctg aagagcgcgc
     8521 ccgcaagccc gagaagctgc cgcttcgaca tcagcccggc gccgagcacg cccgacccgg
     8581 tcgcgaccga gcggtggtcg gcacgggcgc gcacctcggc gcccgactcc tcgtcgtaga
     8641 tgtcgttgat cacgttggcg gccagatgca tcgccgccgc gccggcgacc gtgagcgcga
     8701 agcgcggcca cgaccacacg ccggatcgtt cccacccgac cgccgcgccg acggtcacgg
     8761 gcgtgagcat gaccggcagc accttcgcgc gggtggcccg cacgaacagc ttcatgcccg
     8821 tctcgtttgc catcagcccg gcctcccggc gtcgtcgcgc gcgacaggct acgggcggct
     8881 tgctcgcgcc gcaaagcgca cgcgccgggg gcctctcccc gcctacgatg gccggacacg
     8941 atgacgatcg agaccccgat cccctccggc ggcttgctcc gccgcaatcc cgggttcgct
     9001 cgcctgtacc tgtcggccat cgtgtccttc gcgggcgact ggttcgcggt cgtcgcgctc
     9061 caagggctgg tgctcgaaac cacgggcaag cccgcgctcg ccgggctgat gctggcgacc
     9121 tcgacgctgc cgttcgcgat cgtctcgccg ttcgccggcg tgatcgccga tcgcgtggac
     9181 cgccgcaagc tcatgatcgg tgccgacctc gcgcgcgcgg cggtcgcgct cggcttcctg
     9241 tttgcgcgcg acgcgtccac gatctggatc gccttcgtgt gcacggcctt gctcagcgcg
     9301 ttcgcgccgt tcttcgagcc ggcctcctcg gcgtcgttgc cgaacctggt ccggcgacag
     9361 gatctggcgc gcgcgaacgt gctgatgggt tcggcgtggg gaaccatgct cgcagtgggc
     9421 gccgcgctcg gcggcctggt cgccgccacg ctcggccgtg aagcggcctt cctgatggac
     9481 tccggatcgt tcctggtgtc ggcgctcctg ttgcgctcga tccgcgagcc gttccaggag
     9541 aagatccccg agccgaccca ggtcacgatc gtgcaggaca ttgcggtcgc cctgcggttc
     9601 gtgcgcgagg aagcgcgggt acgggccatc ttggtggcca aggggctgtt cggctcggcg
     9661 ggcggcacga tcgcgctgct gtccaccatg gcggtgcagg tcttcgagcg cggcgacacc
     9721 ggcatcggca tcctgatggg cgcgcgcggc gtcggcgcgc tcgccggacc gttcctgttc
     9781 cggcgcttcc tgctccgcaa caaggacgag cggctgctgg tgtcgatcgc cttcgcgttt
     9841 gcggtgttct cgatctgcta cgcgctgttc gccggcgcgc ccagcctgtg gctggccgcg
     9901 gccgccgtct gcggcgcgca catcggcggc ggcgccaact ggaccatcag cgcctacgcc
     9961 ctccagcgat tcacccccga ccacgttcgc ggccgcatct tcgccttcga ctactcgctg
    10021 atcacgatga cgatgacgac atcgctgtcg ctcgcgggct tcgccgccgg gatcttcgac
    10081 gtgcgcgcgg tcgcgttcgg cttcgcgatg atcggcatgg tgatgatgct gggctggacc
    10141 gcctgggcgt ggcgcctgat cggcccgcgc gccctgcgcc gggcgcgcga agcaaccgcg
    10201 acctgaccgc gctcgttcga caggctgcgc gctctgcgcg agactgccgt catgcccaat
    10261 cgactcgcca gcgcatccag cccctacctg ctgcagcacg ccggcaaccc ggtggactgg
    10321 tacgagtggg gccccgaggc gcttcagcgc gccaaggacg aggacaagcc gatctttctg
    10381 tccatcggct acgccgcctg ccactggtgc cacgtgatgg agcgcgagtc gttcgaggac
    10441 cccgagatcg ccgcgttcat gaacgaccac ttcgtcaacg tcaaggtcga tcgcgaggag
    10501 cggcccgacc tcgatcagat ctacatgaac gcggtccagg cgatgacggg gcacggcgga
    10561 tggccgatga cgatgttcct cacgcccgac ggggcgccgt tctacggcgg aacctacttt
    10621 ccgcccgagg accggcaggg tctgcccgcc ttccgccgcg tgctcatggg ggtcgccgag
    10681 gcctggcgct cccagcgcga cgaggcgcgc acgcaaagca tcaacctgct cgcgcacatc
    10741 acgaagatga ccgaagggct gcccgcgggt ggcgcgctgg acccgtcgat ccttcgcacg
    10801 gccttcgacg cgatggccgc gaacttcgac cagcagtggg gcgggttcgg cggggcgccg
    10861 aagttcccgc agccgatgac cctggagttc cttttgcgcg cgagccgtct cggcttcgcg
    10921 ggcgcccgcg acatgctgac tttgacgctg acccggatgg cgcgtggcgg catcttcgat
    10981 caggtcggcg gcggcttcca ccgctactcg gtggatggct tctggctcgt gccccacttc
    11041 gagaagatgc tctacgacaa cggccagctg ctgcgcctgt acgcgcgcgc ggccgtcgcg
    11101 ctcgacgatc cgcaccgcct gttcgcgggg acggcgatcg ccaccggcga gtacctgctg
    11161 cgcgagatgc gccatcccgg cggcggcttc tacagcagcg gcgacgcgga cagcgaaggc
    11221 gaggagggca agttcttcgt ctggaccacc gacgagatcc ggcgtatcgc gggcgaggac
    11281 gatgagatcg tgtccgcggc atggtcgctg cagcacggcg ggaacttcga aggcaagacc
    11341 atcccgtgga tgccgctgcc gctcgaggag gtcgcacacg cgaccggcgc gaaggtcgag
    11401 cgcatccgtg ccgccctgga ccgggtcaag ggcgagatgt tcgcgctccg cgagcaacgc
    11461 gtgcggcccg cgatcgacga caagatcctc gcgtcgtgga acggcctggc catcgccggc
    11521 ctgtgcgaag cgggacggct gctggaccgt gacgacttca tcgtcgccgc gatgcaggcc
    11581 gccgacttcg cgctcgcgaa cctgcgccgc gacgacggcc ggctgctgcg ctcgtggcgc
    11641 gacgggcgca cgagcggtcc gggctatctc gatgactacg cgctgctggc cggcgcgctg
    11701 ctcgacgtgt acgagaccac gttccagacg cgctacttca ccgaggcgcg tgcgctcgct
    11761 gatgcgatga tcgagctgtt cgcggccgag gacggcggct tctacgacac cggctcggat
    11821 gccgaggcgc tcgtgctgcg gccgcgcgac gtgttcgaca acgccgtgcc ctccggaagc
    11881 tcatcggcgg cctcgctgct gctgcgccta gccgcgctca ccggggaagc gtcctacgag
    11941 caccgtgcga tcgcatcgct gcagcgcgtc accggcatcg ccgcgcaggc gccgctcggc
    12001 ttcggcgagg cgctgtgcgc catggactcg gctacggcgg gcataaagga gatcgcgatc
    12061 accggcaccg ggccggagac gcgctggctc gccgacgtgg tctggcgccg ggtcgcgctg
    12121 ccgcgggtgc tcgccggcgg cgacgaggag acggtgccgc tgatgaaggg ccgcaccgag
    12181 cgcaacggcg tcgccaccgc ctacgtgtgc gagcgcttcg cctgcaagga gccggtcacc
    12241 gatcgtgacg cgctcgccgc gcagttggac ggctaggcgc gcggtggagt ccggtggtca
    12301 tgcaaccgac tcgtcaagaa aattgacgag tcggccggcg gccatgtgga cacacggcgt
    12361 ggacacacgg cgtggacaca cggcgtggac acacggcgtg gacacacggc gtggacacac
    12421 ggcgtggaca cacggcgtgg acacacggcg tggacacacg gcgtggaca
//
LOCUS       DATLFO010000277         5058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_115870, whole genome shotgun sequence.
ACCESSION   DATLFO010000277 DATLFO010000000
VERSION     DATLFO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115870"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VM841_11330"
     CDS             complement(<1..635)
                     /locus_tag="VM841_11330"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVL90812.1"
                     /translation="MSSLAAVVRGSRRKSDIKPLRRFLLGLAGFRIVIGLLAIPMAAF
                     LYREHFLVLVLMRPTKEVLLFGGFLARQHKIHLLQILIAAVPLAILGVWHAYALGRMY
                     SKEIHDAKLPGIAGRILPAERIKAMTKALKKHGAKVIVIGRVAVFPSSILGMAAGSSK
                     MKAKTFLPADALGGLLSIAEAAGAGYLLGAAYKNGKPWLTAIGLAALVGFG"
     gene            679..1431
                     /locus_tag="VM841_11335"
     CDS             679..1431
                     /locus_tag="VM841_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVL90813.1"
                     /translation="MTTIRAFIRRRLDPAQRFGLRATLLGIAVAAGGIGFGYLVSQVV
                     HNGPLVRVDTAAARNLHEWVSGKGALIVILKVITFLGTPLFFFLIVSIAAGYLLWRHR
                     LRLAVFLVSTSLGGGIVDSIVKIVVDRDRPNLENPLIPLHGKSFPSGHAMSSTFVYGA
                     LLLTFMPVIPKKRRPWVVAGAVVLVLAIGVTRLALGVHYITDIVGGYLLGFVWLTAMT
                     VAFSVWREERGRKPVEPMRGLEPEAKKDLARP"
     gene            complement(1491..1563)
                     /locus_tag="VM841_11340"
     tRNA            complement(1491..1563)
                     /locus_tag="VM841_11340"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1528..1530),aa:Ala,seq:tgc)
     gene            2018..3067
                     /gene="ligD"
                     /locus_tag="VM841_11345"
     CDS             2018..3067
                     /gene="ligD"
                     /locus_tag="VM841_11345"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVL90814.1"
                     /translation="MAAAEMITVEGREVRISNPDKMFFPEIGVTKMDLVRYWMAVAPG
                     ALAGCRDRPTLLKRHPNGVDGDFFYQKRAPTSRPEWIDTVQVTFPSMRTADFLVMRDA
                     AHLAWAINLGCLELHPWPVRASDTDHPDELRIDVDPTPEATWDHVRQTALLVREVLQE
                     HGLEGFPKTSGKRGIHVNVRIEPRWPFTEVRRAALALGREVERRDPMLVTTAWWKEER
                     HGVFIDYNQNARDRTVASAYSVRPTPDGRVSAPLRWDEVATVEPDDHTILTMPERFAG
                     IGDPGAGIDERAYVLEPLLELAARDEAGGLGDAPWPPNFPKAPGEPTRVAPSRAKKQT
                     AHAKKHASAKKKRAR"
     gene            complement(3060..4088)
                     /locus_tag="VM841_11350"
     CDS             complement(3060..4088)
                     /locus_tag="VM841_11350"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HVL90815.1"
                     /translation="MPWPIEPPLKPMLSKAASEIPRGEGWRYEPKWDGFRAIVFRDGD
                     RVKIDSRNARPLDRYFPELLSPLREALPDPCVADGEIVLQGERALDFGTLQMRLHPAA
                     SRINMLAERTPASFVAFDLLHDGERDLRGEPLSLRRARLTERVTVNDTVLLTPHTEDP
                     DEAADWFTRFEGAGLDGVIAKHETIRYVEGERVMVKIKHERTADVVVGGYRIGKDGTS
                     LGSLLLGLYDVHGTLHHVGFTSSFNAKQKREILELLQPLRGEGGFGDGRTPGEPSRWS
                     GGKDMEWVSLQPKLVCEVAFDKLEGDRFRHGARFLRWRPDKDPSDCTYDQLDPPHPFS
                     LEEIRAIT"
     gene            complement(4112..>5058)
                     /locus_tag="VM841_11355"
     CDS             complement(4112..>5058)
                     /locus_tag="VM841_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL90816.1"
                     /translation="QQRRTRGRANRARYAEAPAARRLSVPRPVRSSSGPALLPVIVAA
                     VLSAGIAWIVATRRRRRVERAAAEASLEASRVKDALTNVKHEIGTPVTSILLAARILS
                     SRKISAAERGKIVGKLTDSALRLERTVDLLVTYAALRGGARAVSKAHLDPASLAQASA
                     RRLGELHPGRRIVVRAGESVPAVAGDASLVRAALDQLVDNALKFSRSGEAVEIAVRAA
                     PSSPGSCEIAVRDRGIGIAPADQARIFDEYDQLDGSATREFGGVGLGLAFVREVAGLH
                     GGHVEVRSVPGAGSTFVLRFPPASSRPVVVRMRKRRVA"
BASE COUNT          833 a         1716 c         1728 g          781 t
ORIGIN      
        1 atcccgaacc cgaccagcgc cgccagaccg atcgcggtga gccacggctt gccgttcttg
       61 taggcagcgc ccagcagata accggcgccg gccgcctcgg cgatcgacag cagcccgccg
      121 agggcgtcgg ccggcaggaa cgtcttggct ttcatcttcg acgagccggc cgccatgccc
      181 aggatcgagg acggaaagac cgcgacacgg ccgatcacga tgaccttcgc gccgtgcttc
      241 ttgagtgcct tggtcatcgc tttgatccgt tccgcgggca ggatgcggcc cgcgatgccg
      301 gggagcttcg cgtcgtggat ctccttggaa tacatgcggc cgagcgcgta cgcgtgccac
      361 actcctagga tcgccagcgg cacggccgcg atcaggattt gcagaaggtg aatcttgtgc
      421 tggcgcgcca ggaatccgcc gaacagcagc acctctttcg tcggacgcat cagcacgagc
      481 accaggaaat gctcgcggta taggaacgcc gccatcggaa tggcgagaag gccgatcacg
      541 atgcgaaagc cggcgaggcc gagcagaaac cgccgaagcg gtttgatgtc ggacttcctg
      601 cggctcccgc gaacaaccgc cgcgagcgag gacatgccgc taggtttccc acgcgttggc
      661 cacgggcaaa ccaagcgtgt gaccacgatc cgtgcgttca ttcgacgacg cctcgatccg
      721 gcccagcgct tcgggcttcg cgccacgctg ctcggcattg cggtggccgc cggcggcatc
      781 gggttcggtt acctggtgag ccaggtcgtc cacaacggcc cgctcgttcg tgtggatact
      841 gccgcggccc gcaacctgca cgaatgggtc tcgggcaagg gcgcgttgat cgttatcctc
      901 aaggtcatca ccttcctcgg cacgccgctg ttctttttcc tcatcgtctc gatcgccgcg
      961 ggctatctgc tgtggcgcca ccggctccgg ctcgcggtct tcctggtctc gacatcgctc
     1021 ggcggcggca tcgtggactc gatcgtcaag atcgtggtgg accgggaccg gccgaacctc
     1081 gagaacccgc tgatcccgct gcacggcaag agctttccca gcggccatgc gatgtcgtcc
     1141 acgttcgtct acggtgcgct gctgctgacg ttcatgcccg tgatcccgaa gaagcgacgg
     1201 ccctgggtcg tcgcgggcgc cgtggtgctc gtgctcgcga tcggcgtgac acggctcgcg
     1261 ctcggcgtgc actacatcac cgacatcgtc ggcgggtatc tgctggggtt cgtgtggctg
     1321 accgcgatga cggtcgcatt cagcgtctgg cgcgaggagc gaggccgcaa gccggtcgaa
     1381 cccatgcgcg gcctcgagcc ggaagccaag aaggatctcg cccgccccta gaaagatccc
     1441 ggccaccagg cggcctgtcg gacggtctgt cgaggttcgg tgcccgcgtg tggagacgat
     1501 gggattcgaa cccacgaccc ccggcttgca aagccggtgc tctaccaact gagctacgtc
     1561 cccgatgtcg gcaccgccga ttgtaggcga accggcagga cccaggcgcg tgccgaatgt
     1621 ggtccacccg gtgaggaaag ccttgcttga gcggtttgcc ccttggttcg cgagggaaat
     1681 cgatgccgca atgaacgttc ggcgagtcca ccggaaacgc atccgccggc gcgagagagg
     1741 cagcgatctc gttgcggatc ttcacgcggt gatcgtcgcc aacatcggcg gccccgcggg
     1801 acaacgtgtc tcgatcgcca aacaggttcg cgccgtggtg gccgatcgcc ctcaccgaac
     1861 cggaccccgt cccgaaggac accgttgagc aacgagcagg agtatcgcaa gctcacccgc
     1921 gaagagctcg actgacaagc tcacccgcga agagctcgac tgagcggtgc agccgggcgg
     1981 gctcgccaat caccggcccg ggtatcctcg aatcgcgatg gccgccgccg agatgatcac
     2041 ggtcgaagga cgtgaggttc ggatcagcaa tccggacaag atgttctttc ccgagatcgg
     2101 cgtgacgaag atggacctgg tccgctactg gatggcggtc gcgccgggcg cactggccgg
     2161 ctgccgggac cggccgacgc tgctcaaacg gcacccgaac ggcgtggacg gcgacttctt
     2221 ctaccagaag cgcgcgccga cgagccggcc cgagtggatc gacaccgtcc aagtgacgtt
     2281 tcccagcatg cgcaccgccg acttcctggt catgcgcgac gccgcgcatc tcgcgtgggc
     2341 aatcaacctg ggctgcctcg agcttcatcc gtggcccgtg cgcgcctccg acaccgacca
     2401 tcccgacgag cttcgcatcg atgtcgatcc cacgccggag gcgacatggg atcacgtccg
     2461 gcagaccgcg ctgctggtgc gcgaggtgtt gcaggagcac gggctcgaag gattccccaa
     2521 gacgtccggc aaacgcggca tccacgtcaa cgttcgcatc gagccgcgct ggccgttcac
     2581 cgaggtgcgc cgagccgcgc tcgcgctcgg ccgcgaggtg gagcggcggg atccgatgct
     2641 ggtgacgacc gcgtggtgga aggaggagcg gcacggcgtg ttcatcgact acaaccagaa
     2701 cgcgcgtgac cgcacggtcg cctccgccta ctcggttcgg cccacgcccg acgggcgcgt
     2761 gtcggcaccg ctgcgctggg acgaggtcgc gaccgtcgag ccggacgacc acaccatcct
     2821 gacgatgccg gagcgcttcg ccggcatcgg cgatccgggg gcggggatcg acgagcgcgc
     2881 gtacgtgctg gagccgctgc tcgagctcgc ggcccgggac gaggcgggcg ggctcggcga
     2941 tgcgccgtgg ccgccgaact ttccgaaggc acccggtgag ccgacgcgcg tagcgccgtc
     3001 gcgcgccaag aaacagactg cgcatgcgaa gaaacatgcg tccgcgaaga agaagcgcgc
     3061 taggtgatcg cgcggatctc ctcgagcgag aacgggtgcg gcggatcgag ctgatcgtag
     3121 gtgcagtcgg acggatcttt gtcggggcgc cagcgcagga agcgcgcgcc gtgacggaac
     3181 cgatcacctt cgagtttgtc gaacgcgacc tcgcagacca gcttcggctg aagcgagacc
     3241 cactccatgt ctttgccgcc gctccagcgg ctcggctcgc ccggcgtgcg gccgtccccg
     3301 aagccgcctt cgccgcggag cggctgcagc agctcgagga tctcgcgctt ctgcttggca
     3361 ttgaacgacg aggtgaagcc gacgtgatgg agcgtcccgt gcacgtcgta gaggccgagc
     3421 agcagcgaac cgaggctcgt gccgtccttg ccgatccggt agccaccgac cacgacgtcg
     3481 gcagtgcgtt catgcttgat cttgaccatc acgcgttcgc cctcgacgta gcgaatcgtt
     3541 tcgtgtttgg cgatcacacc gtccaggccg gcgccttcga agcgcgtgaa ccagtcggcg
     3601 gcttcgtcgg gatcctcggt gtgcggggtg agcagcacgg tgtcgttgac cgtgacgcgc
     3661 tcggtcagac gcgctcgccg cagcgacagc ggctcgccgc gcaggtcccg ctcaccgtcg
     3721 tggagcagat cgaaggcgac gaacgatgcg ggcgtgcgct ccgcgagcat gttgatgcgt
     3781 gaggcggcgg gatgaagccg catctgcagc gtgccgaagt ccagcgccct ttcgccttgc
     3841 agcacgatct cgccgtcggc cacgcacgga tccggcagcg cctcgcggag cggggagagc
     3901 agttccggaa aatagcggtc gagcggccgc gcgttgcgtg aatcgatctt gacgcggtcg
     3961 ccgtcgcgga acacgatcgc acggaagccg tcccacttcg gctcgtagcg ccagccttcg
     4021 ccgcgcggaa tctcggacgc ggccttggaa agcatcggct tgaggggcgg ttcgatcggc
     4081 cagggcattg ccgcggcagc ctagcagcgc actacgcgac gcgacgcttg cgcatccgca
     4141 ccacgaccgg cctgctcgac gccggaggga agcgcaggac gaacgtgctg ccggccccgg
     4201 gcaccgagcg gacctctacg tgtccgccgt gcagccccgc gacctcgcgc acgaacgcaa
     4261 gacccagccc gacgccgccg aactcgcggg tcgccgagcc gtcgagctgg tcgtattcgt
     4321 cgaagatgcg cgcctggtcg gccggcgcga tgccgatgcc ccggtcgcgc acggcgatct
     4381 cgcaggatcc gggtgacgac ggggcggctc gcacggcgat ctcgacggct tcgccgctgc
     4441 gcgaaaactt cagcgcattg tccacgagct gatcgagcgc ggcgcgcacc aggctcgcgt
     4501 cccctgcgac cgcgggcacg gactcgccgg cccgcactac gattcgccgg ccaggatgca
     4561 gttcgcccag ccgcctggcc gacgcttgcg cgagcgaagc cggatcgaga tgcgccttcg
     4621 acacggcgcg cgcaccgccg cgcagcgccg cgtaggtcac gagcagatcg acggtgcgtt
     4681 cgaggcgcag tgccgagtcg gtgagcttgc cgacgatctt gccgcgctcg gcggccgaga
     4741 tcttgcgaga cgacaggatg cgcgccgcga gcaggatcga ggtgaccggc gtgccgatct
     4801 cgtgcttgac gttggtgagg gcatccttga cgcgcgaggc ttcgagcgag gcttctgcag
     4861 cggcgcgttc aacgcggcgg cgccggcgcg tcgcgacgat ccatgcgatc ccggccgaca
     4921 gcacggccgc cacgataacg ggcagcagcg caggacccga ggacgagcga accgggcgcg
     4981 gtacgctgag gcgccgggcg gccggcgcct cagcgtaccg cgcccggttc gctcgtcctc
     5041 gggtcctgcg ctgctgcc
//
LOCUS       DATLFO010000278         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_115928, whole genome shotgun sequence.
ACCESSION   DATLFO010000278 DATLFO010000000
VERSION     DATLFO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115928"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VM841_11360"
     CDS             <1..1382
                     /locus_tag="VM841_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVL90817.1"
                     /translation="AMLRRLLLIGVALALTAAPMSSNAFGNGTGFKTGSCELAALGVA
                     PGSSGRVLTVGQGQAYGTIQDAVDAANEGDHIVIHPGIYAEHVRIIDKPGLRVTGTDR
                     DTVIIDGGSSNSRRIGIEVKRSDRVLLENMTGRQHQLHSFYWESSTGYWGRYLTAYNQ
                     GDYGIFAYDSRCGQFDNTYSSGAQDGGIYIGECFPCDAVVTNIESTENGLGYSGTNAG
                     GNLILKDSWWHNNGLGIVPNSLDGEVAPPQRGTTIKNNLIENNSNLQAPGRGAAADFY
                     GVGIAIAGGNQNQIIGNTVRNHALAGIVMAPLATESSKPYLASSNIVWGNTVSGSGLA
                     DLAQGATAGPNNCWSHNTYGTSAPVALQTIWGCTNTDPESLPYYPGITPPGGDPRVEM
                     GFVEGIAGLNGRNASRSDWKTWPTPTCGTPGPNQRYAPNSCINLPDETGDGTYANDGA
                     PDIWLWALL"
     gene            1544..>2412
                     /locus_tag="VM841_11365"
     CDS             1544..>2412
                     /locus_tag="VM841_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HVL90818.1"
                     /translation="MASRPYHVQGERDACGIGFVADARGRSSRMIVEAALGALRRVKH
                     RGAVAADAKTGDGAGILLPIPGRFFARAAAGLGLAEPDPEWIGIAMVFAEPAVPELDV
                     SRIVEAACRAEAIDVIAWRTVPVEPGALGDFARATMPRIAQAMLLRPAGLSREEAERN
                     AHRARRRAEAAIRKAGLAVSFPSFGFSTVTYKGLVAADQLGELYPDLRDPDVEAHFAI
                     FHQRYSTNTLPTWERAQPFRLICHNGEINTIAGNVNRMRCREGRLGKWSLLEEEVLRP
                     VIDESGSDSAMLD"
BASE COUNT          398 a          858 c          815 g          341 t
ORIGIN      
        1 tcgcaatgct cagacggttg ttgctcatcg gcgtcgcgct ggccctgacg gccgcgccga
       61 tgtcgtcgaa cgcgttcggc aacggaaccg ggttcaagac cggatcctgc gagctcgccg
      121 cgctgggcgt cgcgcccggc agcagcggac gcgtgctcac cgtcggacag ggccaggcat
      181 acggcacgat ccaggacgcc gtcgacgcgg cgaacgaggg tgatcacatc gtcatccatc
      241 ccggcatcta cgccgagcac gtccgcatca tcgacaagcc gggcttgcgc gtgaccggca
      301 ccgatcgcga cacggtcatc atcgacggcg gatcgtccaa cagccgccgc atcgggatcg
      361 aggtcaagcg ctcagaccgg gtcctgctcg agaacatgac cggccgccag caccagcttc
      421 actccttcta ctgggagtcc tcgacgggct actggggccg ctacctgacc gcctacaatc
      481 agggcgacta cggcatcttc gcctacgact cccgctgcgg acagtttgac aacacgtact
      541 cgtcgggcgc gcaggacggc ggcatctaca tcggcgagtg cttcccctgc gacgcggtcg
      601 tgaccaacat cgagtccacc gagaacggcc tgggctactc gggcacgaac gcgggcggca
      661 acttgatcct gaaggactcg tggtggcaca acaacgggct cgggatcgtg ccgaactcgc
      721 tcgacggcga ggtcgccccg ccccagcgcg gcacgacgat caagaacaac ctgatcgaga
      781 acaactcgaa cctgcaggcg ccgggccgtg gcgcggccgc cgacttctac ggcgtcggca
      841 tcgccatcgc gggcggcaac cagaaccaga tcatcggcaa cacggtgcgc aaccacgcgc
      901 tggccggcat cgtgatggcg ccgctggcga ccgaatcgtc gaagccgtat ctggccagca
      961 gcaacatcgt gtggggcaac accgtgagcg gcagcgggct ggccgacctg gctcagggcg
     1021 ccaccgccgg tccgaacaat tgctggagtc acaacaccta cggcacgtcg gcgccggtgg
     1081 cgctgcagac gatctgggga tgcaccaaca ccgaccccga gtcgctgccg tactatccgg
     1141 gcatcacgcc gcccggcggg gacccgcgcg tcgagatggg cttcgtcgag ggcatcgccg
     1201 gcctgaacgg ccgcaacgcc tcgcgctcgg actggaagac gtggccgacg cccacctgcg
     1261 gcacgcccgg cccgaaccag cggtacgccc cgaactcgtg catcaacctg ccggacgaga
     1321 ccggcgacgg cacctatgcc aacgacggcg cccccgacat ctggctctgg gcgctgctgt
     1381 agattcgccc cgcagcgaag cgagcgaggc ccggcgaccc cgggcctcgc ttctttcgcg
     1441 ataacggttg cgaaacggtt ttcgcccggt agactcgatc gcaacgggca acggcgctca
     1501 cgacgggcgc cgtttttgtt ttgccgatct ccggaggggt ttcttggcga gcaggccgta
     1561 tcacgtgcag ggcgagcggg acgcatgcgg gatcggattc gtcgcggatg cgcgcggccg
     1621 ctcttcgcgg atgatcgtcg aggcggcgct cggcgcgctg cggcgggtca agcaccgcgg
     1681 tgccgtcgct gccgacgcca agaccggcga cggcgcgggc atcctgctgc cgatccccgg
     1741 ccgcttcttc gcgcgggccg ccgccggcct gggcctggcc gagcccgacc ccgaatggat
     1801 cgggatcgcg atggtcttcg ccgagcccgc cgtgcccgag ctcgacgtga gccgcatcgt
     1861 cgaggccgcc tgccgcgccg aggcgatcga cgtgatcgcg tggcggaccg tgccggtgga
     1921 gcccggcgcg ctcggtgatt tcgcgcgcgc gacgatgccg cgcatcgcgc aggcgatgct
     1981 gctgcgtccg gcgggcctgt cgcgcgagga ggccgagcgc aacgcgcatc gcgcccgccg
     2041 ccgcgccgag gccgcgatcc gcaaggccgg cctcgcggtg tcgttcccct cgttcggctt
     2101 ctccaccgtc acctacaagg ggctggtggc ggccgaccag ctcggcgagc tctaccccga
     2161 ccttcgcgac cccgacgtcg aagcgcactt cgcgatcttc catcagcggt actcgaccaa
     2221 cacgctgccg acgtgggagc gggcgcagcc gttccggctg atctgccaca acggcgagat
     2281 caacaccatc gcgggcaacg tcaaccggat gcgctgccgc gagggccggc tcggcaagtg
     2341 gtcgctgctc gaggaagagg tcctgcggcc ggtgatcgac gagagcggaa gcgacagcgc
     2401 gatgctcgac aa
//
LOCUS       DATLFO010000279         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_15944, whole genome shotgun sequence.
ACCESSION   DATLFO010000279 DATLFO010000000
VERSION     DATLFO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15944"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VM841_11370"
     CDS             complement(<1..654)
                     /locus_tag="VM841_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90819.1"
                     /translation="MSLITHVRAREVLDSRGNPTVEVDVVTDDGALGRAIVPSGASTG
                     AHADNTVDVQEFMIAPVGAAGFSEALRWGAEVYHALKSVLKKDGKSTAVGDEGGFAPD
                     LKSNEDAITALMTAIEKAGLQPGDEVAIALDVAATELFKDGSYHFEGEGTTRTAEAWA
                     ETLDDWLGRYPIVSIEDPFSEDDWDGWALATERLGGKTQLVGDDLFVTNPVRLRMGIE
                     "
     gene            complement(817..1647)
                     /locus_tag="VM841_11375"
     CDS             complement(817..1647)
                     /locus_tag="VM841_11375"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HVL90820.1"
                     /translation="MERISRGFRIVGASWQVLKADRELLVLPIISFLATALMAATFFF
                     PVAMTMPDDGTVQIQHYLLMGVFYFIATFITVFFNAAVVAAAMIRLNGGDPTLSDGLR
                     AAWSKVGKIAAWSLLTASVGLILRMLEERAGFLGRIVIAIVGAAWAAITFFVVPVLLF
                     EPVEVVPGVKRSASLFRERWGEQFTGNVSIGMAMFLLLLPVLALTFMLFSASALVGIV
                     FGVVAIGLLTSAGSAMSGIFNAALYKYATTGQVAAGFAPDDLSGAFKPKKDAGRGAWS
                     "
     gene            complement(1669..>2284)
                     /locus_tag="VM841_11380"
     CDS             complement(1669..>2284)
                     /locus_tag="VM841_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HVL90821.1"
                     /translation="RVAAARAAVLRAPRRSLHARVRGFAGTRPALLLAFGWGAAEALV
                     WPIVPDLLVAALALAAPRRAIPIAAAAVAGSVAGGLAAYGLGALGAAGVLDHVPLVTG
                     RMQAEAASRMTTDGAAGVLGQPWSGIPFKVFAYQAPAAGVDAGAFAVFAFLARAVRFL
                     QVAAMFAAGAILLRRWIDRLYAWTLGAVLVVFYYGLSRVVAAWS"
BASE COUNT          395 a          771 c          803 g          315 t
ORIGIN      
        1 ctcgatgccc atacggaggc gcacggggtt ggtgacgaac aggtcgtcac caacaagctg
       61 agtcttgccg ccgagccgtt cggtcgcgag cgcccagccg tcccagtcgt cctcgctgaa
      121 cggatcttcg atcgacacga tcggatagcg gccgagccag tcgtcgagcg tttcggccca
      181 ggcttcggcg gtgcgtgtcg tgccttcgcc ctcgaagtga taggagccgt ctttgaacag
      241 ctcggtcgcc gcgacgtcga gcgcgatggc gacctcgtcg ccgggctgca gaccggcctt
      301 ttcgattgcg gtcatcagcg cggtgatcgc gtcctcgttg gacttgaggt cgggcgcgaa
      361 tccgccttcg tcgccgacgg cggtggactt gccgtctttc ttgagcacgc tcttgagcgc
      421 gtggtagacc tccgcgcccc agcgcagcgc ctcactgaag ccggccgcgc cgacgggcgc
      481 gatcatgaac tcttgcacgt ccacggtgtt atcggcgtgc gcgccggtgg aggcgccgga
      541 cgggacgatg gcgcggccga gtgcgccgtc gtcggtgacg acatcgacct caacggttgg
      601 gtttccgcgg gagtccagga cctcgcgggc gcgaacgtgc gtgatcaggc tcatggcgcg
      661 attctaatga gcggtgccgt agcggggcga accggggtcg ccgtccgtcg ctcgtcgtgc
      721 agggaccgtc cgtcgttcgt gtgcaggagc cgtccgtcgt tcgttgtgca ggccgtcgct
      781 atccgcagcc ggctgcacaa cgaacgagac gaacccctag ctccaggcgc cgcgaccggc
      841 gtccttcttc ggcttgaacg cgccgctgag gtcgtcgggc gcgaatccgg ccgcgacctg
      901 gcccgtggtc gcgtacttat agagcgccgc gttgaagatc ccgctcatcg ccgagccggc
      961 cgacgtcagc agcccgatcg ccacgacgcc gaacacgatg cccacgagcg ccgacgccga
     1021 gaacagcatg aacgtcaaag ccaggaccgg cagcaacaga aggaacatcg ccatcccgat
     1081 cgagacgttg ccggtgaact gctcgcccca gcgctcccgg aacaggctcg ccgaacgctt
     1141 cacgccgggc acgacctcga ccggctcgaa cagcaggacc ggcacgacga agaaggtgat
     1201 cgcggcccag gcggcgccga cgatcgcgat gacgatccgg ccgaggaagc cggcgcgctc
     1261 ctcgagcatg cgaagaatca agccgacgct cgccgtcagc agcgaccagg ccgcgatctt
     1321 gccgaccttt gaccaggcgg cgcgcaagcc gtccgacagg gtcgggtccc cgccgttgag
     1381 ccggatcatg gcggcggcga cgaccgcggc gttgaagaag acggtgatga aggtcgcgat
     1441 gaagtagaag acacccatga gcaggtagtg ctggatctgc accgtgccgt cgtccggcat
     1501 ggtcatcgcg acgggaaaga agaacgtggc ggccatcaaa gcggtcgcga ggaacgagat
     1561 gatcggcagc accagcagct cgcggtcggc cttgaggacc tgccacgacg cgccgacgat
     1621 ccggaatccc cgggagatgc gttccatgtc cttcgctcct cgctcgaatt acgaccaggc
     1681 ggcgaccact cggctcagcc cgtagtagaa gacgacgaga accgccccga gggtccaagc
     1741 atagagccga tcgatccatc ggcgcagcag gattgcgccc gcagcgaaca tcgccgcgac
     1801 ttgaaggaat cgcacggcgc gcgccaggaa ggcgaacacg gcgaacgcac cggcgtccac
     1861 gccggcggcc ggggcctggt aggcgaagac cttgaacggg atgccgctcc agggctggcc
     1921 gagcacgccg gccgcgccat cggttgtcat gcggctcgcg gcttcggctt gcatgcgtcc
     1981 ggtcacgagc gggacgtggt cgagcacgcc ggcggcgccg agcgccccga gcccataggc
     2041 ggccaggccg ccggcaaccg atcccgcgac ggcggcggcg gcgatcggga tcgcgcgacg
     2101 cggcgccgca agcgcgagcg cggcgacgag gaggtccgga acgatcggcc agacgagcgc
     2161 ctcggccgcg ccccagccga aggcgagcag caacgcgggt cgtgttccgg cgaagccgcg
     2221 cacgcgggcg tgcaggcttc gccggggagc ccgaagcacg gcggcgcggg cggcggcgac
     2281 tcgc
//
LOCUS       DATLFO010000280         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_65676, whole genome shotgun sequence.
ACCESSION   DATLFO010000280 DATLFO010000000
VERSION     DATLFO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65676"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..150)
                     /gene="rpmJ"
                     /locus_tag="VM841_11385"
     CDS             complement(37..150)
                     /gene="rpmJ"
                     /locus_tag="VM841_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVL90822.1"
                     /translation="MKVRPSVRKMCAKCKVIRRNGRVMVICTDPRHKQRQG"
     gene            complement(311..1072)
                     /gene="map"
                     /locus_tag="VM841_11390"
     CDS             complement(311..1072)
                     /gene="map"
                     /locus_tag="VM841_11390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVL90823.1"
                     /translation="MIFRKTPDEIQKMRRAGAVVAETLAVLREAVRPGVSTRELDEIA
                     EREITGRGAIPSFKGYRGFPATICTSVNYEIVHGIPGDVVLRDGDLIKLDCGAIVEGY
                     HGDSAITLPVGEVEESARKLIEATERALWAGIQEARPGNRIGDIGAAVQTVAEGAGFS
                     VVREYVGHGIGRALHEDPPVPNYGTARKGLPLEEGLVIAIEPMVNVGSFETRLLPDGW
                     TVVTADGNLSAHFEHTICITAEGPEVLTALREVAA"
     gene            complement(1069..1704)
                     /locus_tag="VM841_11395"
     CDS             complement(1069..1704)
                     /locus_tag="VM841_11395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HVL90824.1"
                     /translation="MRIILLGPPGVGKGTQGVRLCERLGIPHIATGDLLRFAVKWQTE
                     IGNRAKRYMDAGELVPDEIVLELLRTRLSQPDALDGFVLDGFPRNPAQADALGEILDE
                     LGHKLNGVVSLEVPDDEIVERLSARASCPTCGRTFTIKGGEPEVCDVDGERLFQRDDD
                     RPEVIRKRLQVFHQQTSPLVEYYEKQGLLVRVNGVGDVDEIQARMLEAVPG"
     gene            complement(1710..>2553)
                     /gene="secY"
                     /locus_tag="VM841_11400"
     CDS             complement(1710..>2553)
                     /gene="secY"
                     /locus_tag="VM841_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVL90825.1"
                     /translation="FGTLTVGKTFLVVLTLTAGTALIMWLGELVTQRGIGNGMSILIF
                     ASIISSMPSESTSLWANAGAVKGSVFILLGIAIVLTVVLVEQAQRRIPVQYAKRVVGR
                     KTYGGSSTYIPMKVNQAGVIPIIFASSLLYLPVLASTIITNNAFKEFVDRHFASGSSI
                     WYVGFYFLLIIFFTYFYTAITFNPTDVADNMKKYGGFIPGIRPGKPTADYLSYTLTRI
                     TLPGAIFLGTIAVLPFIAQGIWNVTSFPFAGTAVLIMVGVGLETMKQIESQLMMRHYE
                     GFLK"
BASE COUNT          444 a          831 c          828 g          450 t
ORIGIN      
        1 aggtcaacgc ctgcaatacg agccattcga ttctccctac ccttgccgct gtttgtgtcg
       61 cggatctgtg caaatcacca tgactcgccc gttccggcgg atcactttgc acttcgcgca
      121 catcttgcgc acgctcggac gcaccttcat gacagcctcg attcgttcct tggggtgaaa
      181 ctcggctcgc cggtcagacc gcgaacacct gcgcccgtgt catccattcc gcagccggcg
      241 cagacagcct tgcctcggag ccgaggggtc atactaccag gggaaacggc ggggaccaac
      301 cggaagtcgc tcacgcggcc acctcccgca gggcggtgag gacctcaggg ccttcggccg
      361 tgatacagat cgtgtgttcg aagtgagcag acaggttgcc gtcggccgtc acgaccgtcc
      421 atccgtccgg cagcaggcgg gtctcgaagg agccgacgtt gaccatcggc tcgatggcga
      481 tgaccaggcc ctcctcgagt ggaagaccct tcctggccgt gccgtaattc ggaaccggcg
      541 gatcttcgtg cagcgcgcgc ccgatcccgt ggcccacgta ctcgcgcacc acggagaatc
      601 ccgcgccctc ggcgacggtt tggaccgccg cgccgatgtc gccgatgcgg ttgcccgggc
      661 gggcctcctg gatgccggcc cacagcgcgc gctcggtcgc ctcgatcagc ttgcgggcgg
      721 actcctccac ctcgccgacc ggcagagtga tcgccgagtc gccgtgatac ccctcgacga
      781 tggcgccgca gtccagcttg atgaggtcgc cgtcgcggag cacgacgtcg ccggggatgc
      841 cgtggacgat ctcatagttc accgacgtgc agatcgtggc cgggaagccg cggtacccct
      901 tgaacgaggg gatcgcgccg cggccggtga tctcgcgctc ggcgatctcg tcgagctcgc
      961 gcgtgctgac gccgggtcgg accgcctcgc ggaggaccgc gagggtctcc gcgacgacgg
     1021 cgcccgcgcg ccgcatcttc tggatctcgt ccggcgtctt ccggaagatc aacccggcac
     1081 cgcctcgagc atgcgcgcct ggatctcatc gacatcgccg acgccgttga cgcggacgag
     1141 gagaccctgc ttctcgtagt actcgaccag cggcgaggtc tgctggtgga agacctggag
     1201 tcgcttgcgg atgacttcgg gccggtcgtc gtcgcgctgg aagagccgct cgccgtcgac
     1261 gtcgcacacc tcgggctcgc cgcccttgat cgtgaacgtc cgcccgcagg tcgggcagga
     1321 cgcgcgcgcc gacaaccgct cgacgatctc gtcgtcgggc acctcgaggc tcacgacgcc
     1381 gttgagcttg tgtcccagct cgtcgaggat ctcgccgagc gcgtcggcct gcgcggggtt
     1441 tcgcgggaag ccgtcgagga cgaatccgtc gagggcgtcg ggctgcgaca agcgcgtgcg
     1501 caagagctcc agcacgatct cgtcgggcac gagctcgccg gcgtccatat agcgcttcgc
     1561 gcggttgccg atctcggttt gccacttcac cgcgaagcgc agcaagtcac ccgtcgcgat
     1621 atgtgggata cccagacgct cgcacaggcg gacgccttgg gttcctttcc cgacgcccgg
     1681 aggtcccaga agaatgatgc gcatgaggcc tacttcagga acccttcgta gtgacgcatc
     1741 atcagctgcg actcgatctg cttcatcgtc tcgagtccga cacccaccat gatcagcacc
     1801 gccgtcccgg caaacgggaa cgaggtgacg ttccagatgc cttgcgcgat gaacggaagg
     1861 acggcgatcg ttccgaggaa gatcgcgccc ggcagggtga tccgcgtcag cgtgtacgag
     1921 aggtagtcgg cggtcggctt acccggccgg atgcccggaa tgaatccgcc gtacttcttc
     1981 atgttgtctg cgacgtcggt cgggttgaac gtgatcgccg tgtagaagta cgtgaagaag
     2041 atgatgagca ggaagtagaa gccgacgtac cagatgctcg agccgctcgc gaagtgacga
     2101 tcgacgaact ccttgaaggc gttgttcgtg atgatcgtcg aagcgaggac cggcaagtac
     2161 agcagcgacg acgcaaagat gatcgggatg acgccggcct ggttgacctt catcgggatg
     2221 taggtcgagg agccgccgta ggtcttccgc ccaacaactc gtttcgcgta ttgcactggg
     2281 atccttcgct gcgcctgttc taccaagacc accgtgagca cgatcgcgat cccgaggagg
     2341 atgaataccg accccttcac cgcgccggcg ttggcccaca gggacgtcga ttcgctgggc
     2401 atggacgaga tgatcgaagc aaagatcagg atggacatcc cgttgccgat ccctcgttgg
     2461 gtgaccagtt cgccgagcca catgatgagg gcggttcctg cggtcagggt gagcacgacg
     2521 aggaacgtct tgccgacggt cagggtgccg aag
//
LOCUS       DATLFO010000281         9429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_96416, whole genome shotgun sequence.
ACCESSION   DATLFO010000281 DATLFO010000000
VERSION     DATLFO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96416"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1398)
                     /locus_tag="VM841_11405"
     CDS             complement(244..1398)
                     /locus_tag="VM841_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90826.1"
                     /translation="MFDIENAIQRARDVASTAPLAERAETVAALLRGADALLAEVAAL
                     IRRGGVDAVAGRPLEHVLRHDARVTAWDASALKQAADTLQHMPSLTDALQDGYVSWSQ
                     VRAIVSALRSVPVAERARIDGIVGEQAKRLAEADPERLVELVEDEAARSRPDLAVGRE
                     DRAIRSSFLAIQGCLDGTATIYGQGDAVSIATIVDALDAVADDPVHPDAGVTRAQQRY
                     DALVHLCEATLAGESAGSRPRPRVLATIDVRDADDGARILWSLAGRRPRVSCVTRDEL
                     LCDATIVPVLFDGREVLGVGDQSNVFSDKVRTAIVARDQRCRFCSRAAASWCDVHHLV
                     PGKGNAARDGCLLCRRCHRTVHRAKWTATWQDGGALKFERRGKHFISLPP"
     gene            complement(1475..1612)
                     /locus_tag="VM841_11410"
     CDS             complement(1475..1612)
                     /locus_tag="VM841_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90827.1"
                     /translation="MKALLIGLFIALPLALAVWTIPHLRRAFNQYRANPWTPKRWDDD
                     E"
     gene            complement(1725..2201)
                     /locus_tag="VM841_11415"
     CDS             complement(1725..2201)
                     /locus_tag="VM841_11415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HVL90828.1"
                     /translation="MTVESTTLRTVMGRFTTGVAVVSSTRDGEPVALTANALASVSLD
                     PPLVLFCPDKESKTLRGILDSGVFAVNVLHDGQEDLARRFAASGPKNFDGVPWRPGHT
                     GAPILKDCLAWLDCSIWAQYEGGDHLILVGLVKAAEINADRPPLVFYRSGYHTIAP"
     gene            complement(2212..4581)
                     /locus_tag="VM841_11420"
     CDS             complement(2212..4581)
                     /locus_tag="VM841_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVL90829.1"
                     /translation="MADPYRREGQGRDLTHPAIEALLAERDELRRQRLSGLDSVHGLS
                     RAMDVALSSLWSEASGDNGVALVALGGYGRGELSPRSDVDLVFLYRGRKKPEELTKRV
                     LYALWDAGFEVGHATRTVKDSLKLAAENHEAHTSFLSARHVAGDGDLFAEFAGAVDAA
                     TRKRADAFAREVAEAAAARHRSAGEVPFRLEPNIKDGAGGLRDLHLASWLARYFDTCD
                     PSVVAGNAEILHRIRNWLHDATGRRTDVFLLAHQDAAAADLGYEGERPGDALMRDYYT
                     AARAIWWHSQSCLAATAPTVANTHPHPDVAIFEAGAWTPDTRRAFVAALRSGSARALE
                     ELDQANVFARMLPEWDLVSSLPQRNIYHRYTVDVHCFETVAQAVALQDDDGLAGQVWR
                     DLADHDRMLLAALFHDAGKGAAEDHSERGERIARAWTRAIGFGDGAADEVAWLVRNHL
                     LLAETATRRDIGDQRLVESLAAQVGIAERLKMLYVLSVADARATGPEAWTPWKAALVA
                     ELFGRVLHVLERGEVVGADVDEIIKRRTAEVREALSDREPGEIDAHLLGMTRAYVLAF
                     PTPDLVRHFALTSPPPGEGDLRMHAAQAGEPGVWDVTMVARDRPGLFAILSGALAVSG
                     INIIGAQAFTRLDGVAIEMFQVVGAMDRVFDDERWAKVERTCREALAGTVDLETEIAE
                     RRRAYTRASKGKREPTRIEIDNLISDFATVIEVHATDRVGLLFDITRALADFGLDIQV
                     ARIATYGDDVVDAFYVRDLLGQRLDEPARIEQVRDGLLARIGGGARQRG"
     gene            complement(4539..4877)
                     /locus_tag="VM841_11425"
     CDS             complement(4539..4877)
                     /locus_tag="VM841_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVL90830.1"
                     /translation="MKLVTAVVKPFKLEEVKESLRPVGIQGMTVVESRGFGRQRGHTE
                     VYRGAEYQVDFVPKVRIEILCDEGQVEQVVAAILESGKTGSIGDGKIWVTPVENVWRI
                     RTGEKGKDAI"
     gene            complement(4888..6138)
                     /locus_tag="VM841_11430"
     CDS             complement(4888..6138)
                     /locus_tag="VM841_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVL90831.1"
                     /translation="MNGADTAWILVSSALVMFMTPGLAFFYGGLVRSKNVLATIMQSF
                     VALGVISIIWAVVGYSIGFADGNRFFGYLDFFGLSNVGADPAVIAGTELTIPGTTYMV
                     FQLMFAVITPALISGAFAERMKFSGYLLFISLWSIFVYSPVAHWVWGGGWLGLGEGNV
                     QALDFAGGTVVHINAGVAALACILYMGKRKGYGKEAMHPHNVPMVITGAAILWFGWFG
                     FNAGSALAANGLASAAFVNTHLGAAGALVGWLIAEWGRNKVPTSIGAATGAVAGLVAI
                     TPAAGFVEPWAAIVIGLISGAVCYAFVALKPKIGYDDSLDVVGVHFVGGIIGALLTGV
                     FASEGVGGFDGLLYGNAAQLGRQLIAVIATIVYSFIVTMVILKIVDATTGLRVKEDDE
                     RTGLDLSQHAESAYTLIDVGTHKS"
     gene            6325..7338
                     /locus_tag="VM841_11435"
     CDS             6325..7338
                     /locus_tag="VM841_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVL90832.1"
                     /translation="MKRGTVVRPVLESALLRGNPLGDPADRITPVYLPPSYEEQPGRR
                     YPLILAITGFMGNGRMHLNESFLDDSLDARLDRLIDSGAMGEAIVVMVDAMTRYGGSQ
                     FVDSSATGPYGRYVIEEVIPWADATFRTIPHRDARGIFGKSSGGYGSIRLAMDHPDVL
                     GAFACHSGDMAFEYCYLTEWPKTLRYLWHNDLTPKQFLDTFRERLDRGEGYFSTLNDL
                     AMASCYSPDPGSDLGFDLPFDLETGEVIDEVWQRWLAHDPVRIIDERVEALRSQRLVF
                     IDCGKKDEWFLDVGARWAVSKIHSLGIDVVHEEFDAGHMSIPYRYDRSLPLLTKALLP
                     PEG"
     gene            7344..7889
                     /locus_tag="VM841_11440"
     CDS             7344..7889
                     /locus_tag="VM841_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVL90833.1"
                     /translation="MNPISSIDVSALADAVRRRHRACGADAATLASLENHMYRVLALV
                     EDLSEGESLNTLEYSLLRTSAILHDAAAGKTASPDASAVLASEMLVAVGADAEFTAAV
                     AKAIRRHGGSRIGADSTMPQSHAEQLLHDVCLLARIQEARDGESESGLRGLASMLCTP
                     TAVRMAGHMIGERSGAAVPAL"
     gene            7979..8818
                     /locus_tag="VM841_11445"
     CDS             7979..8818
                     /locus_tag="VM841_11445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL90834.1"
                     /translation="MHQSEHEAIRWQTGVWDTISDIYERQIDRRFAPVVEHTLTRAGL
                     ASGHSVLDLGTGTGSAALKAAQLVAPGGSVLGVDLSAEMLEIAHGRAERAGLRNVSFR
                     EGRAEELPAEDDTFDAIVSSLCFMYVIDRAAGAAESARVLKPSGRFVAAVWGGPDECD
                     IVLFQSTAGSFAPPPPVTGVGPGALADPSSLVAHLRDAGIEARVESETLSFEFPDFET
                     AWDVLAGVTTAKLTDERRDEAKAAVRDVMWPDPNEPRVFVNTTQFVVGTRIESAARSR
                     SRA"
     gene            8974..>9429
                     /locus_tag="VM841_11450"
     CDS             8974..>9429
                     /locus_tag="VM841_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL90835.1"
                     /translation="MPASDLIAMLAAQAPPDLTSVALHPTGEAALHAAIAAAGPGAVA
                     TLSHPATPIPGALRAHSPYCFRCPLGLSFPSCEIACLDSLASIVDTGDVAAVVIEPAA
                     QVPGGMITAPPGHLRRVRDLCDTHGAALIADERFTGAGAGGFFWAFTRDG"
BASE COUNT         1499 a         3319 c         3114 g         1497 t
ORIGIN      
        1 cattttcatg atcgaagaca ccgacaatct gttccgcttc atcgactcgc tcgccaaggt
       61 cgtcagcggc cgcgtcttcg ccgtccagag cgacgacctc ggagtcgacg tgcttcggga
      121 ctacgtcaac caccgccgca cggcatagat cagcggcacc tcctcgctct caacctggtc
      181 agcctatagc cgaaggcatg ccgaaagggc caccccgggg gcggtggaga ggtctgggtg
      241 ttgttacggc gggaggctga tgaagtgctt gccgcggcgc tcgaatttga gggcgccgcc
      301 gtcctgccag gtggcggtcc atttggcgcg gtggacggtg cgatggcagc ggcgacacaa
      361 caggcaaccg tcgcgtgcgg cgtttccctt gccgggaacg agatggtgga cgtcgcacca
      421 cgacgcggca gcccggctgc agaaccggca tcgctggtcg cgcgcgacga tggcggtgcg
      481 gaccttgtcg gaaaagacgt tcgattggtc gccgacgccg agcacctcgc ggccatcgaa
      541 cagaaccggc acgatggtgg cgtcgcagag cagctcgtca cgggtaacgc aactcacgcg
      601 cgggcggcga ccggccagcg accacaagat gcgcgctccg tcatcggcgt cgcgcacgtc
      661 gatggttgca agaacgcggg gccgcgggcg agagccggcg gattcgccgg cgagggtcgc
      721 ctcgcagaga tgaacgagcg cgtcatatcg ctgctgcgcg cgcgtcacgc cggcgtcggg
      781 atgcacggga tcatcggcga ccgcatcgag cgcgtccacg atcgtggcga tcgagaccgc
      841 atcgccctgt ccgtagatgg tggccgtccc atcgaggcat ccttggatcg cgagaaagct
      901 cgaccggatc gcgcggtctt cgcgacccac agcgaggtcc gggcggctgc gcgcagcttc
      961 gtcctcgacg agctcgacga gccgctctgg atcggcctcg gcgagccgct tcgcctgctc
     1021 gccgacgatc ccatcgatac gcgcgcgctc ggcgacggga accgatcgta gcgccgacac
     1081 gatcgcccgc acctggctcc acgagacgta cccgtcttga agcgcgtcgg tcagggaagg
     1141 catgtgctgc agggtgtcgg cggcttgctt cagcgccgag gcatcccacg cggtcacgcg
     1201 cgcatcgtgg cggagcacgt gctcgagcgg gcggccggca accgcgtcga cgccgccccg
     1261 ccggatcaac gccgccacct cggccagcag cgcatcggcg ccgcgcagca acgccgccac
     1321 ggtctccgca cgctcggcca gcggcgcagt cgatgcaaca tcacgcgcgc gttggatggc
     1381 attctcgata tcgaacacat gttcgactat attagtgtat cagtatactg tcaagctatt
     1441 ttcgggatgg cccccgcctc cggcgaccgg ccgcctactc gtcgtcgtcc cagcgcttcg
     1501 gcgtccaggg attcgcgcgg tattgattga acgcgcgacg caggtgcggg atcgtccaaa
     1561 cggcgagggc gagcggcaag gcgatgaaca agccgatgag caaagctttc atggcgactg
     1621 tgttcccatc ctgcgtcgag acgaatcgac cgcgctcggc tttagcgcgg accggcagcc
     1681 cggcgatccc cgattgacgt cccgcgcccg cagcgactga ccatctatgg cgcgatcgtg
     1741 tggtagccgc tgcggtagaa gaccagcggc ggacggtcgg cgttgatctc ggcggccttg
     1801 accaggccga ccaggatcag atgatccccg ccctcgtatt gcgcccagat ggagcagtcg
     1861 agccacgcga ggcagtcctt caggatcggc gctcccgtgt ggccgggtcg ccacggcacg
     1921 ccgtcgaaat ttttcgggcc gctggcggcg aagcggcggg cgagatcttc ctggccgtcg
     1981 tgcagcacgt tgaccgcgaa cacgccgctg tcgaggatcc ctcgaagcgt cttggattcc
     2041 ttgtcggggc aaaacagcac cagcggcgga tccagcgaca cgcttgcgag cgcattggca
     2101 gtcagcgcga ccggctcgcc gtcgcgggtc gaggagacga ctgcgacgcc cgtggtgaac
     2161 cgtcccatca cggtgcgcag cgtcgtggat tcgacggtca tcgaatccac tctatccccg
     2221 ttggcgggcc cctcccccga tccgggcgag caaaccatcc ctgacctgct cgatccgggc
     2281 gggctcgtcc agccgctgtc cgagcagatc gcggacgtag aatgcatcca cgacgtcgtc
     2341 gccgtaggtg gcgatgcggg cgacctggat atccagcccg aaatctgcca gcgcgcgtgt
     2401 gatgtcgaac agcaacccga cgcggtcggt cgcgtggacc tcaatgacgg tagcgaagtc
     2461 gctgatcagg ttgtcgatct cgatgcgggt cggctcgcgc ttgcctttcg acgcgcgggt
     2521 gtaggcgcgg cgacgttcgg cgatctcggt ttccaggtcg accgtgccgg ccagcgcctc
     2581 gcggcatgtt cgctcgacct tcgcccagcg ctcgtcgtcg aagacgcgat ccatcgcgcc
     2641 cacgacctgg aacatctcga tcgcgacgcc gtccagacgc gtgaatgctt gcgcgccgat
     2701 gatgttgatg cccgacaccg ccagcgcgcc cgacaggatc gcgaacaggc cgggccggtc
     2761 gcgcgcgacc atggtgacat cccacacgcc gggctcaccc gcctgcgcgg cgtgcatgcg
     2821 aaggtcgccc tccccgggcg gcgggctggt gagcgcgaag tgccggacca gatccggcgt
     2881 cgggaacgcc agcacgtacg cgcgggtcat gcccagcaga tgcgcgtcga tctcgccggg
     2941 ctcgcggtcg ctcagcgcct cacggacctc ggcggtacgc cgcttgatga tctcgtccac
     3001 gtcggcgccg acgacttcgc cgcgctcgag cacgtgcagc acccggccga acagctcagc
     3061 caccagcgcc gccttccacg gcgtccacgc ctcgggtccg gtcgcgcgcg catcggcgac
     3121 ggacaacacg tagagcatct tgagccgttc ggcgatgccg acctgcgcgg cgagcgactc
     3181 gaccagacgc tgatcgccga tgtcgcgcct ggtcgcggtt tcggccagca gcagatggtt
     3241 gcgaacgagc caggcgacct cgtcggcggc gccgtcaccg aagccgatcg cgcgcgtcca
     3301 ggcgcgcgcg atccgctcgc cgcgctcgga gtgatcttcg gccgcgccct tgcccgcgtc
     3361 gtggaacaac gcggccagca gcattcggtc gtggtcggcc aggtcgcgcc atacctgccc
     3421 ggccagcccg tcgtcgtcct gcagcgcgac ggcctgcgct acggtctcga agcagtgcac
     3481 gtccacggtg tagcggtgat agatgttgcg ctgcgggagg ctgctgacca gatcccattc
     3541 gggcagcatg cgcgcgaaca cattcgcctg gtccagctct tccaatgcgc gggccgagcc
     3601 ggatcgcagc gccgcgacga acgcgcgccg cgtgtcgggc gtccacgcgc cggcctcgaa
     3661 gatcgcgacg tcggggtgcg ggtgcgtgtt cgcgacggtg ggcgcggtcg ccgccaggca
     3721 cgactgcgag tgccaccaga tcgcgcgcgc ggcggtgtag tagtcgcgca tcagcgcgtc
     3781 accgggccgc tcgccttcgt agccgaggtc cgccgctgcg gcgtcttgat gcgcaagcag
     3841 gaacacgtcg gtgcggcgtc cggttgcgtc gtggagccag ttgcggatgc gatgaaggat
     3901 ttcggcattg cccgcgacca cggacggatc gcacgtgtcg aagtaccggg ccagccacga
     3961 cgcgaggtgc agatctcgaa ggccacccgc gccgtccttg atgttcggct cgaggcggaa
     4021 cggcacctcg ccggcggagc ggtgccgcgc cgcggccgct tcggccacct cgcgcgcgaa
     4081 ggcatcggcc cgctttcggg tcgcggcatc gacggcgccg gcgaactcgg cgaacagatc
     4141 accgtcgccc gcgacgtgcc gcgccgacag gaacgaggtg tgggcctcgt gattctcggc
     4201 cgcgagtttg agcgagtcct tcacggtgcg cgtggcgtgc ccgacctcga agcccgcatc
     4261 ccagagcgcg tacagcacac gcttcgtcaa ctcctccggc ttcttgcgcc cgcggtagag
     4321 gaataccaga tcgacgtcgg acctcgggga cagctcaccg cggccgtagc cgccgagcgc
     4381 caccagcgcg accccgttgt cgccgctcgc ctcgctccac agcgacgaca gcgcgacatc
     4441 catcgcgcgc gagagcccgt gcacggagtc gagcccggaa agacgctgcc ggcggagctc
     4501 gtcccgctcc gccagcagcg cctcgatcgc gggatgggtc agatcgcgtc cttgcccttc
     4561 tcgccggtac ggatccgcca cacgttctcg accggcgtga cccagatctt gccgtcgccg
     4621 atcgagccgg tcttgcccga ctcgaggatc gcggcgacga cctgctcgac ctgtccttcg
     4681 tcgcacagga tctcgatgcg caccttcggc acgaagtcga cctggtactc ggcgccgcga
     4741 tacacctcgg tgtgcccgcg ttgccggccg aatccgcggg actcgacgac ggtcatgcct
     4801 tggatgccga ccggacgaag cgactccttc acctcttcca gcttgaaggg cttcaccact
     4861 gccgtgacca gcttcatccg actctcctta gctcttgtgc gtcccgacgt cgatcagcgt
     4921 gtaggcgctt tcggcgtgct gcgacaggtc caggccggtt cgctcgtcgt cttccttcac
     4981 gcgcaggccc gtcgtggcat ccacgatctt gaggataacc atcgtgacga tgaaggagta
     5041 cacgatcgtg gcgatcaccg cgatgagctg acggccgagc tgtgcggcgt ttccgtagag
     5101 cagaccgtcg aacccgccga ccccttccga cgcgaacaca ccggtgagca gcgcgccgat
     5161 gatgccgccg acgaagtgca caccgacgac gtcgagcgag tcgtcgtagc cgatcttcgg
     5221 cttgagcgcg acgaaggcgt agcacaccgc gcccgagatc aagccgatca cgatcgccgc
     5281 ccacggctcg acgaagcccg cggcgggcgt gatcgcgacc aggcccgcga ccgcgccggt
     5341 cgccgcgccg atcgaggtcg gcaccttgtt gcgtccccac tcggcgatca gccagccgac
     5401 cagcgcgccg gcggcgccca aatgcgtgtt gacgaacgcc gcggacgcga gcccgttggc
     5461 cgccagcgcc gagccggcgt tgaagccgaa ccatccgaac cacaggatcg ccgcgccggt
     5521 gatgaccatc ggcacgttgt gcggatgcat ggcctctttg ccgtagccct tgcgcttgcc
     5581 catgtacagg atgcacgcca gcgccgcaac gcccgcgttg atgtgcacga ccgtgccgcc
     5641 ggcgaagtcg agtgcctgca cgttgccctc gcccaggccc agccagccgc cgccccacac
     5701 ccaatgcgcc accggcgagt acacgaagat cgaccacagc gagatgaaca gcaggtagcc
     5761 gctgaacttc atccgctcgg cgaacgcgcc gctgatcagc gccggcgtga tcaccgcgaa
     5821 catcaactgg aagaccatgt aggtcgtgcc cgggatggtg agctcggtgc ccgcgatcac
     5881 cgccgggtcg gcgccgacgt tcgacaggcc gaagaagtcc agatagccga agaagcggtt
     5941 gccgtcggcg aagccgatcg agtagccgac caccgcccag atgatcgaga tcacgcccag
     6001 cgcgacgaac gactgcatga tcgtcgcgag cacgttcttg ctccgcacca ggccgccgta
     6061 aaagaaggcc aggccgggcg tcatgaacat cacgagggcg ctcgacacca aaatccatgc
     6121 ggtgtccgcg ccgttgatct ctgccggcat ccccacctcc tctgagtcgg cggacgcaca
     6181 gacggtccgc aacgccgaga ccggccgcct caagcgattg acgacgagcc tagccctcgt
     6241 gtgtttcacg gccttttcgc ctctgttacg gctcggtaaa cgatgtccgg ggatcgcgca
     6301 tgcctcgtgc taggctcggt ccctatgaag cggggaaccg tggtccggcc cgtcctggag
     6361 tccgcgctgc tacgcggcaa tccgctcgga gaccccgcag accgcataac cccggtctac
     6421 ctgcccccct cgtacgaaga gcagcccggc cgccggtatc ccctgatcct cgccatcacc
     6481 ggcttcatgg gaaacggccg gatgcacctg aacgagagct tcctcgacga ctctttggac
     6541 gcgcggctgg accggctcat cgattcgggc gcgatgggtg aggcgatcgt ggtgatggtg
     6601 gacgcgatga cgcgctacgg cggttcgcag ttcgtcgact cctccgcgac cggcccctac
     6661 ggccggtacg tgatcgagga ggtcatcccc tgggccgacg cgacgttccg caccatcccg
     6721 catcgcgacg cccggggcat cttcggcaag tcctccggcg gctacggctc gatccggctg
     6781 gcgatggacc atcccgacgt gctcggtgcc ttcgcgtgcc attcgggcga catggcgttc
     6841 gagtactgct acctgaccga gtggcccaag acgctccgtt acctgtggca caacgatctg
     6901 acgcccaagc agttcctcga cacgttccgc gagcggctcg accgcggaga gggatacttc
     6961 tccaccctca acgacctcgc gatggcctcg tgctactcgc ccgatcccgg ctccgatctc
     7021 ggattcgacc tgccgttcga cctggagacc ggcgaggtga tcgacgaggt gtggcagcgc
     7081 tggctcgccc acgatccggt ccggatcatc gatgagcgcg tcgaggcgct gcggtcgcag
     7141 cggctggtct tcatcgattg cggcaagaag gacgagtggt tcctcgacgt gggcgcacgc
     7201 tgggcggtgt cgaagattca ctcgctgggt atagacgtcg tacacgaaga gttcgacgca
     7261 ggacacatga gcattccgta tcgctacgac cggtcgctcc ccctgctgac gaaggctctt
     7321 ctgccgcccg aggggtgagg ttcgtgaatc cgatcagctc gatcgacgtc tctgcgctcg
     7381 ccgacgccgt gcgccgccgc caccgtgcct gcggggccga cgccgcaacg cttgcttcct
     7441 tggagaacca catgtatcgg gtcctggctc tcgtcgagga cctgtccgag ggcgagagcc
     7501 tgaacacgct cgagtactcg ctgcttcgca cgtccgcgat cctgcacgac gccgccgccg
     7561 gcaagaccgc ctcaccggac gcgtccgcgg tgcttgcttc ggagatgctg gtcgccgtcg
     7621 gcgccgacgc cgagttcacc gcggccgtcg ccaaggcgat tcgccgccac ggcggctccc
     7681 ggatcggcgc ggactcgacc atgccccagt ctcacgccga gcagctgctt cacgacgtgt
     7741 gcctgctggc ccggatccag gaagcccggg acggggagtc cgaaagcggc ctgcggggcc
     7801 tggcctcgat gctctgcact ccgacggcgg tccgcatggc cggacacatg atcggcgaac
     7861 gcagcggcgc agccgtccct gccctgtagg agcgccaccc gccgcacccg ccgggccccg
     7921 ccgggcttga attcgcgccc gcatcgccgc atgatcgcga gcggaaggag cgaccgcgat
     7981 gcaccagtcc gaacacgagg ccatccggtg gcagaccggg gtctgggaca caatctccga
     8041 catctacgaa cgacagatag accggcgttt tgcgcccgtc gtcgagcaca cgctcacgcg
     8101 ggccggtctc gcgtccggcc attcggtctt ggatctcgga accggcacgg gatccgcagc
     8161 gctgaaggcc gcgcagctcg tcgcgcccgg cggctccgtg ctcggagtcg atctgagcgc
     8221 cgagatgctc gaaatcgccc atggtcgcgc cgagcgagca ggcctgcgca acgtatcgtt
     8281 ccgcgaaggc cgcgccgaag agcttccggc cgaggacgac acgttcgacg cgatcgtgtc
     8341 cagcctgtgc ttcatgtacg tcatcgaccg tgccgccggc gccgccgaat ccgcacgcgt
     8401 gctcaagccg tccggccgtt tcgtggcggc cgtgtgggga ggacccgacg agtgcgacat
     8461 cgtgcttttc cagtccacgg cgggaagctt cgcgccacca ccgccggtga cgggggtcgg
     8521 gccgggcgcc ttggccgatc cgtcctcgct ggtcgcgcat ctccgcgatg ccggcatcga
     8581 ggcgcgcgtc gagtccgaga cgttgtcgtt cgagtttccc gacttcgaga ccgcgtggga
     8641 cgtgctcgcc ggcgtcacga ccgcaaagct caccgacgag cgaagagacg aagcgaaggc
     8701 ggccgtgcgc gatgtgatgt ggcccgaccc gaacgagcct cgggtcttcg tgaacacgac
     8761 gcaattcgtg gtcggaacgc gcatcgaaag cgcggcccgc agccgatccc gggcttgacc
     8821 gagatcagcc ttgaccgtgc cgggccggat acgcagcagc gccgcgatct cctcctcgct
     8881 tcgaccttcc ggagagcgca gcaccccgca ccatcgtcgt gcgcaaccat gccttcgcgc
     8941 tcccggcagg gttcgtccat atcattgtgg ctcatgcccg catccgatct gatcgccatg
     9001 ctcgccgcgc aagcgccgcc ggacctgacg tcggtcgcgc ttcacccgac cggggaggcg
     9061 gccttgcacg ccgcgatcgc cgccgcgggt ccgggcgcgg tcgcgaccct gtcgcatccc
     9121 gcgacgccga tcccgggcgc gcttcgcgcg cactcgccct actgctttcg ctgcccgctc
     9181 ggactgagct ttccgtcgtg cgagatcgcg tgcctggact cgctggcctc gatcgtggac
     9241 accggcgacg tcgcggccgt cgtgatcgag ccggccgcgc aggtgccggg cggcatgatc
     9301 accgctcctc cgggtcatct gcggcgtgtg cgcgacctgt gcgacacgca cggcgcggcg
     9361 ctgatcgccg acgagcgctt caccggcgcg ggtgcgggcg ggttcttctg ggcgttcacg
     9421 cgcgacggc
//
LOCUS       DATLFO010000282        16885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_135719, whole genome shotgun sequence.
ACCESSION   DATLFO010000282 DATLFO010000000
VERSION     DATLFO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135719"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VM841_11455"
     CDS             <1..1103
                     /locus_tag="VM841_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90836.1"
                     /translation="QGFGLEEAYAGALVSLGVPFAITGDHPSLDELQRYPAVIWVAGS
                     TQRKNSVDIRDRAKLSSYLNGGGRLLITGSRTISGIARVDAEWLPANLGLNHKNIWQY
                     GTGAAHGNGLKFHADLAPGRSLMDEYEVVFEDERPDGFGEVSEVYRLDPEVEWEAEGT
                     VGVRYAAGKFKTETHSFSLGQLRDGEVRQQILKSTLAYFGVPLGGRAADASPQIHHAP
                     WRVRLNGQATPVVATVSDADGLAEVTLRYRAGAAGAWTIVPMLESNAANVFEAMIPAS
                     AVTPAGFEYAIGAVDLTGVKAAGDIHAVASAIGPGFGAYPSIVSPRAPSQVKGVKKAA
                     PLPATGVGTAPMVALLMVLAAGTALWLRRRPA"
     gene            1497..2126
                     /locus_tag="VM841_11460"
     CDS             1497..2126
                     /locus_tag="VM841_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HVL90837.1"
                     /translation="MVRRRTQHRSRSQKLLLSAAILGCAASIAGLGTFASFTSTTSAS
                     QTVASGTVTIALGATGASTNRLTVNATGIAPGDTIQRSFTLANTGSQDLASVTVSTSA
                     TSSSLLDTDATDGLQMVIERCSVAWVEAGISPAFTYTCAGTTSTVLASRAVIGSNLSL
                     SNLTATTAGNTDHLRLTMTLPASAGNALQNQSSTIQYSFTGTQRAGTNK"
     gene            2137..2757
                     /locus_tag="VM841_11465"
     CDS             2137..2757
                     /locus_tag="VM841_11465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVL90838.1"
                     /translation="MRVVLPAQRSATKVALRFVSRWMGRVLLGATGAVLIAVAIGPRT
                     GAYQTLTVLTGSMRPSIAPGSVVVVRPVPADAILVGDVVTMNAPVPGSPVVTHRIVEI
                     VEPGAAPVIRTQGDANSHPDPWLARIEGGRVWKVAYVVPAVGRVLTALRSPLVRPLLV
                     YAVPAMLAAWWLVQLWGGSRPCRIRPRRGGTPRAGAALSRGCHAHP"
     gene            2744..3181
                     /locus_tag="VM841_11470"
     CDS             2744..3181
                     /locus_tag="VM841_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90839.1"
                     /translation="MRIRDLGLIAVVLAFTAPLPPASGAFDSKPPPPSMSVATATLAA
                     PSSSSATNGACVVATSRIVNVSWSQTTSSFADGYLVQRATSASGPFSNVATITSHATT
                     VWADSGVAASTTYHYRVLATKLAWRSAASPVSSVTTPSLLCVL"
     gene            complement(3228..3368)
                     /locus_tag="VM841_11475"
     CDS             complement(3228..3368)
                     /locus_tag="VM841_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90840.1"
                     /translation="MPGPGSVDQVCVPFPIIGCFLAVGLFVGDGVALFDGALPAGSPI
                     VR"
     gene            complement(3412..4722)
                     /locus_tag="VM841_11480"
     CDS             complement(3412..4722)
                     /locus_tag="VM841_11480"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL90841.1"
                     /translation="MTLHIETEHEKERARALRAAVVARVESRADELADAMLVAYQQEI
                     GAYGFLDDPAALAEVRAGTRMNLGVVLRAVANGSQLTDAELSALKEIGHRRAAQGFPL
                     HGLLRAYQVGTRLAWQRILDALEAEDTDGSAAARLIAEVSVAVMGIMAQISSAATEAY
                     LETERATAASEERLRRDLFNELLAGPLAASRPLRERAERVGYRLGDVNVVVVAAPEGG
                     TADDGGLRSLAAALRTVRLMSLEPVVDARHGVAIAIVPVPTGISELRIKEAVEKALAG
                     AFPRDVALCAAIGRPEGGASGVAASYRQALRTLDHARLIGRTDSVIGYLASIPSMLLR
                     EDVSAAMDLYRSTIEPLAKQDAEQGTQLVHTLAALLEERGNVLAAAKRLFVHRHTLSA
                     RIDRIEELTRRSLRSRDDLLLLELGLRARDVLPDASADQAPRDS"
     gene            4903..6045
                     /locus_tag="VM841_11485"
     CDS             4903..6045
                     /locus_tag="VM841_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVL90842.1"
                     /translation="MRRILFPLLVGLLVLGVVLGAFAGYLITSNPPPRQDSPGWLRVK
                     SMPGARGEFAAGVVEAQPGGPRLCPGTPCPERLVYAVGGLSGPLAKVLDRVQVFDPAN
                     AWRDGPPLPEARHHLAAAGLGDTLYVTGGTPDKRGLEPHANVWVLRPGRTSFERLADM
                     PEGRVAHAMVAVNKKLYVVAGRGPSSSVLIFDPAKGWSKGAAMPGPRDHVAAVAVQNK
                     IYVIGGRDSSVSRRVDIYDTATDSWSEGVPLPDAISAGAAAALDDGLIHLVGGEDPRM
                     LGGRVYDLHLAFDPIGSVWLEGPKPLLAVHGGAYADVTGKLLVIGGSRRQGTFSIFGW
                     TGLVQVFDPQQIGGGLSPTPSPNPTPSPVPTGPAPTGPTPTGGLPG"
     gene            complement(6062..7717)
                     /locus_tag="VM841_11490"
     CDS             complement(6062..7717)
                     /locus_tag="VM841_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVL90843.1"
                     /translation="MRSIRGLAAAALIVSTIAPPGAPPASASSCSWPMYGRTAGRSFA
                     QDCTSIDAGNVARLVPKWVVPVADSVTASPAVADGVLYVGAWDGTFYAIDAGDGTVLW
                     TFSIDDAHRTAFGRIVSSAAIDTVAVPGYGTVEVVLFGGGATLYALRHSRSGPEVLAR
                     VDVDPRTPALRAAQTADPPQIEIESSPVVGHFPDGDRIYVGMDVHNIANVGRTGLLSF
                     GLRKNESGPTPLRFELLYKFDPETGTVRHSLTDGSGEGFGCGGVWSSPMLASRDGGGV
                     IVFGTSSCKHPNEPDAAGSEAVFGIDAMTGAELWRMRPAHRDRIDDDFGATPNLLPGG
                     VAGAAGKDGVYYARDLATGAPVFTTHAGQAGHLVTDFSVGGMIGSPATGRVRMSSLPD
                     DRRDLIFATTALGTPAGEPLDSGSFPFDSSLAGDPGRLLSLHALDARTGEIVWRTPLS
                     GPSYGAPSLAGDIVFVPVTFQSRVAAFDAATGAPLWSLPTVGAPSSTPVVVGDSLYLG
                     TGTRETDAEFKAFGNEIESLLGSTLGAHPLSPASTISAFRLAP"
     gene            complement(7731..9617)
                     /locus_tag="VM841_11495"
     CDS             complement(7731..9617)
                     /locus_tag="VM841_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL90844.1"
                     /translation="MRKRVLRMLLATVMMAGGLAAADTPYPVIGEPVLRSDTQWIKTG
                     WGYQSKSLTIDTSRRWPVVFTMEGYAGATRPAPNAYLRMDDGELMALRITFRQGPQGD
                     YAMVQASTRGTECSGGSFNLYDRRHAWDGHHIIEWIGTSEWSNGNVGMFGSSFPGQTG
                     YWVASTKPRHLKAVSANLLHSDIYRDIFMPGGVQNYLFPIAWTYAVGPHRLPQDAAQR
                     GPLAEDEICQQNQATRYMAGDLPQAQNEPVWAGVRSVDDQWYSAHAARTYADSIKIPY
                     YQQVNWQDEQVGSRAVVLFNHIDPDPTLIRDKANNLRLIKPKKFVVSNGDHGHGNYAG
                     THRWAFFDIFLRGLPDTTGLMDSQISHYFEAENNGAATTVVHGNTWPHEGTQYVRAYA
                     HEGGSLDFSAPTGPETPDVYLSGASRHGFVQYSQQTGSEVTTAEGYPDAAEWTSAPLT
                     QTTVVGGPLSIDLYASLAGTDTDFFVSVSDVFPDGRVSYLTRGLLKASHRAIDPLRSY
                     YDTDQNGKQIMVQPYRPHSNPQPVMPGEIVKYNIEIFPLGYIFRPGHKIMIKVHTPPV
                     IDGIWGYTPTHHQPAPVTIHHDATHPTSVLLPVVNPAVPANTTTPCPLPGGFACTAPS
                     PLSR"
     gene            9855..11249
                     /locus_tag="VM841_11500"
     CDS             9855..11249
                     /locus_tag="VM841_11500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HVL90845.1"
                     /translation="MTIQRLSALDAQFLYLERPEVHMHVAGIAILDPSTRPDGRLSIE
                     HIVELIRARIHLVPRFRQKIVSPPLGVGRPVWVDDPNFDLSFHIRHAALPAPGGPKEL
                     AAQVQRVHSRPLDRSKPLWEMYFIEGLEDGHVAVLTKSHHAMIDGMSGMDIATVLFDF
                     TPEPREVAEQPYEPQPEPSSRDLFIQSVAEQVAHPVRSAIETIGSALRAPQAAAETVQ
                     RTLGGFAALFARGQAPASPFNVKVGPNRRFAMAEVPVTDAKLIKNELGGKVNDVVLAA
                     VAGALDRLLGGRGIDTRGLTLRTMVPVSTRDAAPQTQLGNKISSLFVDLPIGVEDPVE
                     RLRQIVAATKDVKRSHQAVAAGMLMNLGQWAPPTIHGMAARAVARQRFINLVVSNVPG
                     PQVPLYLAGAKMLVTYPVMPLGETVGLSVAVTSLSGVMGFGFTADWDAVPDIDELPKG
                     LLASIAELKKAASG"
     gene            complement(11252..12568)
                     /locus_tag="VM841_11505"
     CDS             complement(11252..12568)
                     /locus_tag="VM841_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90846.1"
                     /translation="MKHPSRSVVLLLLLAASLAPAAARPGEDPACRLADDAPAFVCTG
                     AGTFRAASVVTAGRGYVVYAFAFTMNGAMLPSAETVKTSLCLFIVTPRGSYTETCRIP
                     ADVSIDPLLDSASIKGRAIDRALGAISIDITVIAGEGLGVIPGAVLASDAGCADAVSR
                     SNTRTVGRRGETRGTITIGRTQTRPAGSTWLSQWAGSDTSTTLSAPCANERAAGDPEA
                     FAFLDCVLGVGPFGIADICNGSWSQRSVTVRARNAAARITMSFVESTSLHAEPPDPTW
                     IAPSEWGPHGDVYGCLRVAVKGRLDAMCDNGFEIEMDPQMGTTRVKGAFDAMDAWDGY
                     YLPQPFPYGRVRVDVTAAIDDKGLRPVGRTRSSSFLDCDGVFDFRRSLLAQGEGTATG
                     TVAFQRRLGTVRGTAARPLPSAMDWEQRSVTAMFGGGYGCAEHEDG"
     gene            complement(12668..13558)
                     /locus_tag="VM841_11510"
     CDS             complement(12668..13558)
                     /locus_tag="VM841_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90847.1"
                     /translation="MQLLEADIDGPVAYADFGGDGPPVVLVHGLGGNHANWMAAAPLF
                     TQVGRVLAPDLPGFGRTPPHGRTSSVHDNRRILHRFITEVAGGRAVLMGNSMGGLLSL
                     MQAAAAPETVASLVLVSPALPRPPRTIGDPLIVATFAAYSMPFAGERLVAARSRRLGP
                     EGLVRETLRVCTVDPARVPETVVAELIEIARWRFNQPWAPTAYLQAARSLVSVVARRA
                     KFRQMIESIEAPGLIVHGRHDRLVPLAASREIVAMRPGWVLEVFEDSGHIAMMEHGDR
                     FAEAALRWVRDQDLRHAPGA"
     gene            13849..15843
                     /locus_tag="VM841_11515"
     CDS             13849..15843
                     /locus_tag="VM841_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90848.1"
                     /translation="MLDRPDPVRHVHTDPPEALPAVPRPPRASAPGEVSAAASTSPAC
                     SSTHSKGYQMLYVYPSDATSRLSSLQSVFLADAAAAQDRIAAESASAPKRPRFFCGIT
                     AVKTNRPGALYTADPLPQILDDLALAGHNRSDRKYLLWWDGPNHPFACGEGTFADDDR
                     SSPSQNYNNTGPDWAVVYKPPGGGSNFCGWTTVLHEIGHTLGSVMHSAPNATGQWHCR
                     DENDVMCYVDNSGLPTFEACPGTYIRFDCNGDDYFNPSPAAGSYLATHWNTYNSSWLE
                     TAPAPSAPDTTITGGPSGSGNAASATFTFTSTIAGSTFTCSLDGAPGQSCTSPKSYSG
                     LADGSHTFTVYATANGVSDPTPASRTWSVGAGGGSSPVPDTSITVKPSSPTKETTALF
                     QFTSTVAGAAFTCTLDGGTPFACSSPQSFTGLAEGPHTFTVAATAGGQPDPSPASHAW
                     TVDTVAPVVTLTAPNVAIQGARSFRVEWAVQDDSEVPVQRVLVRSASTAQQFGWWSVW
                     REQSSPGATFIGGAGWTYCFVIEVFDAAGNRAVNVGQSCTAVPLDDRWTPARGFERRT
                     ENGFFDNSASIARSSGATMTLSNIRAREIDMRVQMCRACRYIDVYWNGQRIKQMSLWT
                     NDPAIVSVRLASFSSLQTGTLQLRIVSPVIVIDSIGVSQY"
     gene            complement(15901..>16885)
                     /locus_tag="VM841_11520"
     CDS             complement(15901..>16885)
                     /locus_tag="VM841_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVL90849.1"
                     /translation="LWEQVSGVPKRLMTFNGDHGTNQDTYVMNDRRAWMDHWMRGIDA
                     GYGTIAQRLTSVDVLQEYNNAPRSTISDTRFPLSTTAWTPYYFRAGGGLSASAPSAGE
                     GSSGYLSGSGRQSWSYQAGPAAGSPFTTADGSDELTFTSAEMTAPLSIAGPITADLYL
                     SSSAPDTALFAQLIDVSPAGARTYLQRGILKASHRAIDLGKSDCVDTSNVRTACDRPG
                     AHLYRPWRPHTNPQPVTPGTVERYLLEIFPVGHVFRPGHKIMVKIMAPPQVESYYSYV
                     AQVPAAMNTLWHDASRPSSITLPVIPGAPVAATGGACGQLEAVRCVARPNG"
BASE COUNT         2549 a         5818 c         5885 g         2633 t
ORIGIN      
        1 accagggctt cgggctcgag gaggcttatg caggcgcgct cgtcagcctc ggggttccgt
       61 tcgcaatcac cggcgaccat ccgagcctgg acgagcttca gcgctacccg gccgtgatct
      121 gggtcgcggg ctcgacgcag cggaagaatt cggtggacat tcgggaccgc gcgaagctgt
      181 cgtcgtatct gaacggtggc ggccgtctgc tgatcaccgg ctcgcgaacg atcagcggca
      241 tcgcgcgcgt ggatgccgaa tggctgccgg cgaacctcgg cctcaaccac aagaacatct
      301 ggcagtacgg caccggcgcc gcgcacggaa acggtctgaa gttccatgcc gacttggcac
      361 ccggccggtc gttgatggac gagtacgagg tcgttttcga agacgagcgt cccgacggct
      421 tcggcgaggt ctccgaggtc taccggctcg atcccgaggt cgaatgggag gccgaaggga
      481 ccgtcggcgt tcggtacgcg gccgggaagt tcaagaccga gacgcactcg ttttcgctcg
      541 gccagctgcg cgacggcgag gtgcgccagc agatcctgaa gtccacgctg gcgtacttcg
      601 gggtgccgct cggcggccgc gcggccgatg cctcgccgca gatccatcac gcgccgtggc
      661 gcgtgcgtct caacggacag gcgacgccgg tcgtggcgac cgtctctgac gccgacgggc
      721 tcgcggaggt cacgctccgc taccgcgccg gcgccgcggg cgcctggacg atcgtcccga
      781 tgctcgagtc gaacgcggcc aacgtgttcg aggcgatgat tcctgcctcg gccgtcacgc
      841 ccgccggctt cgagtatgcg atcggcgcgg tcgacctgac cggggtgaag gcggcgggcg
      901 acatccacgc cgtcgcctcg gcgatcggtc ccgggttcgg ggcctacccc agcatcgtgt
      961 cgccgcgcgc gccgtcccag gtcaaggggg tcaagaaggc cgcaccgctg ccggcgaccg
     1021 gcgtgggcac ggcgccgatg gtggccctgc tgatggtgct cgccgcgggg acggccctct
     1081 ggctgcgccg gcgccccgcc tgaccgaggg aaagacgtgc ggcccggcga gtgatcgccg
     1141 ggccgcaccg tttcgggcgc cgcgtggccg cacgcggcgc cgtggaggtt catgagcccg
     1201 gggggatacg ggcggccgcc ggccgcgccc gccgcagccg ccgtccgatt cccgcatccg
     1261 cccccggctc gaagctcggg tgcgaaagcc gcaccgattc ccattctcgg gcgatcctcc
     1321 cgcttgcttt aggcgacccg agcaattttt cggcgggact ctagtccttt cgttccacct
     1381 caatgatcca ttcgtgccct cgggttgggg gggacccggc cccgatgtga ttgctagcgc
     1441 cgagcaacgc ggccgcaggt ggcaggcgca acacgacgat ctcggaggga tgtctcatgg
     1501 ttcgacgccg gacgcaacac cgcagccgct cgcagaagct tctgctttcc gccgcgattc
     1561 tcggctgcgc cgcttcgatc gcgggactcg ggaccttcgc ttcgttcacc agcacgacgt
     1621 cggcatcgca gaccgtagcc agcggcaccg tcacgatcgc gctcggcgcg accggcgcgt
     1681 cgacgaatcg cctgaccgtg aacgccaccg gcatcgcgcc tggcgacacg atccagcggt
     1741 ccttcactct ggcgaacacg gggagccagg atctcgcctc ggtcacggtg tccacgtcgg
     1801 cgacgtcctc gtcgctgctg gacaccgacg ccaccgacgg tctgcagatg gtcatcgagc
     1861 gctgcagcgt cgcctgggtc gaggccggca tcagtcccgc cttcacctac acctgcgccg
     1921 gaacgacctc gaccgtgctg gcgtcgcggg ccgtgatcgg atccaacctg tcgctgtcga
     1981 acctgaccgc gacgaccgcc ggcaacaccg accacctgcg actgacgatg acgctgcccg
     2041 cgtcggccgg gaacgcgctg cagaaccagt cctcgacgat ccagtactcg ttcaccggca
     2101 cccagcgtgc gggaaccaac aagtaagaca cctcgcatgc gggttgtcct cccggctcag
     2161 cggagcgcca cgaaggtggc gctccgcttc gtatcccgct ggatgggccg ggttctgctg
     2221 ggcgcgaccg gcgcggtgct gatcgcggtg gcgatcggtc ctcggaccgg cgcctaccag
     2281 acgctcaccg ttctgaccgg cagcatgcgg ccgtcgatcg cgccggggtc ggtcgtggtc
     2341 gtgcggccgg tccccgccga tgcgatcctc gtcggcgacg tggtgacaat gaacgcgccc
     2401 gtgcccggca gcccggtcgt cacccaccga atcgtcgaga tcgtcgagcc cggcgccgcc
     2461 cctgtcatcc gcacgcaagg cgatgccaac tcgcaccccg atccttggct cgccaggatc
     2521 gaaggcggcc gcgtgtggaa ggtcgcctac gtcgtcccgg ccgtcggacg ggtcttgacg
     2581 gctttgcggt ctccgctcgt acggccgctg ctggtgtacg cggttccggc aatgctcgcc
     2641 gcctggtggc tcgtccagct ctggggcgga tctcgtccgt gccgcatccg gcctcgtcgc
     2701 gggggcacgc cgcgcgccgg ggccgctctg tcgcgagggt gccatgcgca tccgtgacct
     2761 gggcctgatc gcggtggtgc tggcgttcac ggcgccgctg ccccccgcgt ccggagcctt
     2821 cgactcgaag cctccgccgc cgtcgatgag cgttgcgacc gccacgctgg ccgcaccgtc
     2881 gtcgtcgtcg gcgacgaacg gcgcgtgcgt ggtggcgacc tcccgcatcg tcaacgtgtc
     2941 gtggtcgcag acgacgtcct cattcgcgga cggctatctc gtgcagcgtg cgacctcggc
     3001 gtccggaccg ttctcgaacg tcgcgacgat cacatcgcat gccaccaccg tgtgggccga
     3061 cagcggggtg gctgcctcga cgacgtatca ctatcgcgtg ctcgcgacga agctcgcatg
     3121 gcgcagcgcc gcgtccccgg tgtcatcggt gacgacaccg tcgctgctct gcgtgctcta
     3181 gaacgaccag gcccgctcac gccgggcagg catcgctcgc gatccgatca gcgcacgatg
     3241 ggcgagccgg ccggaagggc gccgtcgaag agcgccacgc cgtctccgac gaagagcccc
     3301 acggccagga agcatccgat gatcggaaac gggacgcaga cctgatccac cgagccgggg
     3361 ccgggcactg agtcgggata tcacgccggg ccggcgacaa tctgcgcggc gtcaggagtc
     3421 gcgcggcgcc tggtcggcgc tggcgtcggg aagcacgtcc cgggcgcgga ggccgagctc
     3481 gagcagcaac agatcgtcgc ggctgcggag gcttcggcgc gtgagctcct cgatgcggtc
     3541 gatccgggcc gagagcgtgt gccggtggac gaagagccgc ttggccgcgg cgagcacgtt
     3601 tccgcgctcc tccagcagcg ccgccagcgt gtgaacgagc tgggttccct gctccgcgtc
     3661 ctgcttggcg agcggctcga tggtcgagcg gtacaggtcc atcgcggcgg acacgtcctc
     3721 gcgcagcaac atcgacggga tcgacgcgag gtagccgatc accgagtcgg tccggccgat
     3781 cagccgcgcg tgatcgagcg ttcgcagcgc ctgccggtac gaagcggcca cgccgctcgc
     3841 gccaccctcg ggtcggccga tcgctgcgca caaggcgacg tcgcgcggga aggcgccggc
     3901 cagcgccttc tccacggcct ccttgatgcg cagctcggat atgcccgtcg gcaccggaac
     3961 gatcgcgatc gcgacgccgt ggcgcgcgtc cacgacgggc tcgaggctca tgagacgaac
     4021 ggtccgcagc gcggccgcga gtgagcggag acctccgtcg tccgcggtgc cgccctccgg
     4081 agccgcgacc accacgacgt tgacgtcgcc gagccggtag cccacgcgct cggctcgctc
     4141 gcgcagcggc cgcgatgcgg ccagcgggcc ggcgagcagc tcgttgaaca ggtcccgccg
     4201 cagccgttct tcgctcgcgg cggtggcgcg ctcggtctcg agatacgcct cggtggccgc
     4261 ggaggagatc tgtgccatga tgcccatgac ggccacgctc acctcggcga tgagcctcgc
     4321 cgccgcggat ccgtcggtgt cctcggcctc gagcgcgtcc aggatgcgct gccacgccag
     4381 ccgggtcccg acctggtagg cgcgcagcaa gccgtgcaac gggaagcctt gcgcggcgcg
     4441 gcggtggccg atctccttca aggccgacag ctcggcgtcg gtcagctgcg agccgttcgc
     4501 caccgcgcga agcaccacgc ccaggttcat ccgggttccg gcgcgcacct ccgccagggc
     4561 tgcggggtcg tcgaggaagc cgtaggcgcc gatctcttgc tggtaggcaa cgagcatcgc
     4621 gtcggcgagc tcgtccgccc gagactccac gcgggccacc acggcggccc gtaaggcccg
     4681 ggcccgctcc ttctcgtgct ccgtctcgat atgcagggtc aaccgaattc ccccttcgcg
     4741 cgtcccccct ggccgcgcat ccggtatccc tggatgtccg attcctagag actggttcga
     4801 caagaaggag cgcttaccct ctcttaaatc gacatgtggt ccagttccgg gtgaagcatc
     4861 ggccttcggc cgccgcaggt ccggtatcct ccccgcgacc cgatgagaag gatccttttc
     4921 cccctcctgg tgggcctgct cgtcctcggc gtggtgctgg gggcgttcgc gggatatctg
     4981 atcaccagca acccgccccc ccggcaggac tcaccgggat ggctgcgcgt gaagagcatg
     5041 cccggggccc ggggggagtt cgccgccgga gtcgtcgagg cccagccggg tggcccacgg
     5101 ctgtgtcccg ggacgccctg cccggagcgg ctcgtgtacg cggtcggcgg cctgtcgggt
     5161 ccactcgcca aggtgctgga ccgcgtccag gtcttcgacc cggccaacgc gtggcgcgac
     5221 ggtccgccac tgcccgaggc gcggcaccat ctcgcggccg ccggcctggg agacacgctc
     5281 tacgtgaccg gaggcacgcc ggacaagcgc ggcctggagc cgcacgcgaa cgtgtgggtg
     5341 ctgcggccgg gacggacctc gttcgagcgg ctcgccgaca tgccggaagg gcgggtcgcg
     5401 cacgcgatgg tcgcggtgaa caagaagctc tacgtcgtag ccggacgggg accgtcgtcg
     5461 agcgttctga tcttcgatcc cgcgaaggga tggagcaagg gcgccgcgat gccgggcccg
     5521 cgtgaccatg tcgcggcggt cgcggtgcag aacaagatct acgtcatcgg cggccgggac
     5581 tcgtcggtga gtcgccgcgt cgacatctac gacaccgcca ccgactcgtg gagcgaaggc
     5641 gttccgctgc cggacgcgat cagcgcggga gctgccgccg cgctcgacga cggcctgatc
     5701 catctcgtcg gcggcgagga tcccaggatg ctcggcggcc gtgtctacga cctgcacctc
     5761 gcattcgatc cgatcggctc ggtgtggctc gagggtccca agccgctgct tgcggtgcac
     5821 ggcggcgcgt acgcggacgt caccggcaag ctgctcgtca tcggcggatc gcgacgacag
     5881 ggaacgttct cgatcttcgg atggacgggc ttggtgcagg tgttcgatcc ccagcagatc
     5941 ggcggcgggc tctcgccgac gccctcgccc aacccgacgc cctcgcctgt gccgacgggc
     6001 ccggcgccga cgggtcccac gcccaccggc ggccttcccg gctgatcgat gaccgccggc
     6061 gctacggcgc cagacggaag gcgctgatcg tcgacgccgg cgacaatgga tgggcgccga
     6121 gcgtgcttcc gaggagcgac tcgatctcgt tgccgaacgc cttgaactcc gcgtcggtct
     6181 cgcgcgtgcc ggttcccagg tagagcgagt cgcccaccac gaccggtgtc gacgacggag
     6241 cgcccacggt cggcaggctc cacagcggcg cgccggtcgc ggcgtcgaag gccgcgacgc
     6301 ggctctggaa cgtgaccggc acgaagacga tgtccccggc aagcgacggc gccccgtacg
     6361 agggtccgga cagcggtgtg cgccacacga tctcgccggt gcgcgcgtcc agcgcgtgca
     6421 gcgaaagcag gcggccggga tcgccggcca aggaggaatc gaacgggaag gagcccgagt
     6481 cgagcggctc gccggccggt gtgcccaacg cggtggtggc gaagatcagg tcgcgccggt
     6541 catccgggag cgaggacatc cgcacccggc cggtcgcggg cgatccgatc atgccgccga
     6601 ccgagaagtc ggtcaccaga tggccggcct gcccggcgtg cgtcgtgaac acgggcgctc
     6661 cggtggcgag gtcgcgcgcg tagtagacgc cgtccttgcc cgcggcgccg gcgaccccgc
     6721 ccggcagcag gttgggtgtg gcgccgaagt cgtcgtcgat gcggtcccgg tgggccggac
     6781 gcatgcgcca cagctcggcg ccggtcatgg cgtcgatacc gaatacggcc tctgatccgg
     6841 ccgcatcggg ctcgttcggg tgcttgcagc tcgaggtgcc gaacacgatc acgccgccgc
     6901 cgtcccggga ggcgagcatc ggcgaggacc acacgccgcc gcatccgaat ccttcaccgg
     6961 atccgtcggt gagcgagtgg cgcaccgtgc ccgtctcggg atcgaacttg tagagcagct
     7021 cgaagcgaag cggagtgggg ccggactcgt ttttccggag accgaagctc agcagcccgg
     7081 tgcggcctac gttcgcgatg ttatgcacgt ccatgccgac gtagatccgg tcgccgtcgg
     7141 ggaagtgccc gacgacgggg ctggactcga tctcgatctg ggggggatcg gcggtctgcg
     7201 cggcacgcag cgccggtgtg cgcggatcga cgtcgacccg ggcgagtacc tcggggccgg
     7261 aacgcgaatg acgcagcgca tacagcgtcg cgcccccgcc gaagagcacc acctcgaccg
     7321 tcccgtagcc cggcacggcg acggtgtcga tggcggcgct ggagacgatg cgtccgaatg
     7381 ccgttcggtg ggcgtcgtcg atcgagaacg tccacagcac ggtcccgtcg ccggcgtcga
     7441 tcgcgtagaa ggttccgtcc cacgccccca cgtacaggac gccgtcggcg acggcgggcg
     7501 acgcggtcac cgagtcggcg accggcacga cccacttcgg cacgagccgg gcgacgttgc
     7561 cggcgtcgat cgaggtgcag tcctgcgcga aggaccggcc ggcggtccgg ccgtacatcg
     7621 gccaggagca tgacgacgcc gatgccgggg gcgcgcctgg cggtgcgatc gtcgagacga
     7681 tcagagccgc tgcggcgagc ccccggatcg aacgcatcat ttctcctcgt ctatcgcgac
     7741 agcggggacg gggcggtgca ggcgaagccg cccggtagcg ggcagggcgt cgtggtgttc
     7801 gccggaaccg ccgggttgac cacgggcagc agcaccgacg tcgggtgcgt cgcgtcgtgg
     7861 tggatcgtca ccggggccgg ttggtggtgc gtgggcgtgt agccccagat gccgtcgatg
     7921 accggcgggg tgtgcacctt gatcatgatc ttgtgacccg gccggaagat gtagccgagc
     7981 gggaagatct cgatgttgta cttgacgatc tcgccgggca tcacgggctg cgggttcgag
     8041 tgcggccggt acggctgcac catgatctgc tttccgttct ggtcggtgtc gtagtacgag
     8101 cgcagcggat cgatcgcacg gtgcgacgcc ttcagaagcc cgcgagtcag gtacgacacg
     8161 cgtccgtcgg ggaacacgtc cgagacgctc acgaagaaat cggtatccgt gcccgccagc
     8221 gacgcgtaca ggtcgatcga caaaggtccg ccgacgaccg tcgtctgcgt cagcggggcc
     8281 gaagtccact cggccgcgtc ggggtatccc tcggcggtgg tgacctccga gcccgtctgt
     8341 tgcgagtact gcacgaagcc gtgacgggat gcgcccgaca ggtagacgtc cggcgtctcc
     8401 ggccccgtcg gggcggaaaa gtcgagcgat ccgccttcgt gggcgtaggc acgcacgtac
     8461 tgggttcctt cgtgcggcca ggtgttgccg tgcacgacgg tcgtggcggc accgttgttc
     8521 tccgcctcga aatagtgcga gatctgcgag tccatcagcc cggtggtgtc cggcaggccc
     8581 cgaaggaaga tgtcgaagaa cgcccatcgg tgcgtcccgg cgtagttgcc gtgtccgtgg
     8641 tcgccgttgg aaacgacgaa cttcttcggc ttgatcagac ggaggttgtt ggccttgtca
     8701 cgaatgagcg tcgggtcggg atcgatgtga ttgaacagaa ccaccgcccg cgagccgacc
     8761 tgctcgtcct gccagttgac ctgctggtag tagggaatct tgatcgagtc cgcgtacgtc
     8821 cgcgccgcgt gcgcggagta ccactggtcg tccacgctgc gcacgccggc ccataccggt
     8881 tcgttctgcg cctgcggcag atcgcccgcc atgtagcgcg tcgcctggtt ctgctggcag
     8941 atctcgtcct cggccagcgg gcctcgctgc gccgcgtcct gcggaaggcg gtgcgggccg
     9001 accgcgtacg tccacgcgat cgggaacagg tagttctgca cgccgccggg catgaagatg
     9061 tcgcggtaga tgtcgctgtg cagaaggttc gcactgaccg ccttcagatg gcgaggcttg
     9121 gtcgaggcga cccagtagcc ggtctggccg ggaaacgagg agccgaacat gccgacgttg
     9181 ccgttggacc actccgaggt gccgatccac tcgatgatgt ggtggccgtc ccaggcgtgg
     9241 cggcggtcgt agaggttgaa cgagccgccc gagcactcgg tgccgcgcgt ggatgcctgc
     9301 accatcgcgt agtcgccctg cggaccctgt ctgaaggtga tgcgcagcgc catcagctcg
     9361 ccgtcgtcca tgcgcagata ggcgttcggc gcgggacgag tcgcgcccgc gtacccctcc
     9421 atcgtgaaga ccacgggcca gcggcgcgag gtgtcgatgg tcaggctctt ggactggtag
     9481 ccccacccgg ttttgatcca ctgggtgtcg ctcctgagaa ccggctcacc gatcaccgga
     9541 tagggcgtat ccgcagccgc cagcccgccg gccatcatca ccgtggccag cagcatgcga
     9601 agcactcgtt tcctcatctg tgatgtcctc cttcggtcga gggacggggt gtcgctgctc
     9661 ggggcgggag cggataccga tgcaggcaac gaacggacaa accttccgct caggaacaat
     9721 tcgccccggg gggccgggat ccttcttggg gggttcgtcc gcatcacgcg caagcgaccg
     9781 attctcgggt ttgaccgggt cccccgggat ggccgacgct gtgcgacacg gcggacgaaa
     9841 ggggcacttt tcagatgacc atccagcgcc tttccgcgct cgacgcgcag ttcctgtacc
     9901 tggaacggcc ggaggtccac atgcacgtcg cgggcatcgc gattctcgac ccttcgacga
     9961 ggcccgacgg cagactctcc atcgagcaca tcgtcgagct gatccgcgcc cgcattcacc
    10021 tcgttccgcg cttccgtcag aagatcgtct ctccgccctt gggtgtgggc cgcccggtct
    10081 gggtggacga tccgaacttc gatctgtcgt tccacatccg ccacgcggcg ctgcctgctc
    10141 ccggcgggcc gaaggagctc gccgcgcagg ttcagcgggt gcactcgcgg ccgctcgacc
    10201 ggtccaagcc gctctgggag atgtacttca tcgaaggcct cgaggacggc cacgtcgcgg
    10261 tgctgaccaa gtcgcatcac gcgatgatcg acggcatgag cggcatggac atcgccaccg
    10321 tcttgttcga cttcacgccc gagccgcgcg aggtcgcgga gcagccgtac gagccgcagc
    10381 ccgagccgag ctcgcgcgac ctgttcatcc agtccgtcgc ggagcaggtc gcgcatccgg
    10441 tgcggtcggc gatcgagacc atcggcagtg cgttgcgcgc accccaggcc gcggccgaga
    10501 ccgttcagcg cacgctcggc gggttcgccg cgttgttcgc gcgcggacag gcgcctgcat
    10561 ccccgttcaa cgtcaaggtg ggaccaaacc gccgcttcgc gatggccgag gttcccgtca
    10621 cggatgccaa gctcatcaag aacgagctcg ggggcaaggt caacgacgtc gtgctggcgg
    10681 ccgtcgccgg cgcgctcgat cgtctgctcg gaggtcgcgg catcgatacg cgtggcctga
    10741 cgctgcgcac gatggttccg gtttcgacgc gcgacgccgc gccgcagacc cagctcggca
    10801 acaagatctc gtcgctgttc gtggatcttc cgatcggcgt ggaggatccc gtcgagcggc
    10861 tgcggcagat cgtcgccgcg accaaggacg tcaagaggtc gcatcaggcc gtggcggcgg
    10921 gaatgctcat gaatctgggg caatgggctc cgcccacgat ccacggcatg gccgcgcgtg
    10981 cggtcgcgcg gcagcgcttc atcaacctgg tcgtctccaa cgtgccgggg ccgcaggtgc
    11041 cgctgtatct ggcgggcgcc aagatgctgg tgacctatcc ggtgatgccg ctcggcgaga
    11101 ccgtcgggct cagtgtcgcc gtcacctcgc tatcgggggt gatgggcttc ggcttcaccg
    11161 ccgactggga cgccgtgccc gacatcgacg agctccccaa gggcctgctc gcctcgatcg
    11221 cggagctcaa gaaagccgcc agcggctgac gctagccgtc ctcgtgctcc gcgcatccgt
    11281 acccaccccc gaacatggcg gtcaccgaac gctgctccca atccatcgcc gacgggagcg
    11341 gccgggcagc ggtcccgcgg acggtcccca gccgtcgctg aaaggccacc gttccggtcg
    11401 ccgtgccctc gccttgggcg agaagcgagc gtcgaaagtc gaacacgcca tcacaatcga
    11461 ggaacgacga tgaccgggtc cggccgacag ggcgcagccc tttgtcgtcg atcgccgcgg
    11521 tcacatccac ccggacgcgg ccgtagggaa acggctgggg aaggtaatac ccgtcccacg
    11581 catccatggc gtcgaaggcc cccttgacgc gagtggtgcc catctgcgga tccatctcga
    11641 tttcgaagcc gttgtcgcac atcgcgtcga gccggccttt cactgccacc cgaaggcagc
    11701 cgtagacgtc accgtgaggg ccccattcgg acggggcgat ccacgtcggg tcgggaggct
    11761 ccgcgtgaag cgacgtggat tcgacgaagg acatcgtgat tcgggcggcg gcgttcctgg
    11821 ctcggacggt cacggatcgt tgggaccagg atccgttgca gatgtccgcg atgccgaagg
    11881 gcccgacgcc gagcacgcag tccaggaacg cgaaagcctc cgggtcgccc gcggcccgct
    11941 cgttcgcgca cggcgccgaa agcgtggtcg aggtgtcgga tccggcccac tgggacagcc
    12001 aggtcgaacc ggccggccgc gtctgcgtcc tcccgatcgt gatcgttccc cgtgtctcgc
    12061 cgcggcggcc gacggttcgg gtgttcgagc ggctcacggc gtcggcgcat ccggcgtcgg
    12121 aggcgagaac ggctcccggt atgacgccga ggccctcgcc ggcgatcacg gtgatgtcga
    12181 tcgagatcgc cccgagcgcc cggtcgatgg cgcgtccctt gatcgaggcg ctgtcgagca
    12241 gcggatcgat gctgacatcg gccggaatgc ggcacgtctc ggtgtagctg ccgcggggag
    12301 tcacgatgaa caggcacagg ctcgtcttga cggtctcggc ggaaggcagc atggctccgt
    12361 tcatcgtgaa cgcgaacgca tacacgacgt atccgcggcc cgcggtcacc accgaggcgg
    12421 cgcggaacgt tcccgcgccc gtgcagacga acgcgggcgc gtcatcggcg aggcggcatg
    12481 cggggtcttc acccgggcgg gccgcggccg gggcgagaga ggccgccagg agcaagagca
    12541 gaaccacgga tcggctcggg tgcttcacgg gtgtccctcc tgttccgagg tctcgcacgc
    12601 gaccatccct gaagagtcaa ttctcgcgaa gctcgatgat tcctgctcat gccccaattg
    12661 gggctgatca ggcgcccggg gcgtgccgga ggtcttgatc ccgcacccag cgcagcgcgg
    12721 cttcggcgaa acgatccccg tgctccatca tcgcgatgtg gccggagtcc tcgaacactt
    12781 cgagcaccca tccgggacgc atcgcgacga tctcgcgtga tgcggcgagc gggacgagcc
    12841 ggtcgtgccg gccgtgcacg atcagcccgg gcgcttcgat ggactcgatc atctgccgaa
    12901 acttggcgcg gcgcgccacg accgagacca gcgatcgggc cgcctgcagg tacgcggtcg
    12961 gcgcccacgg ctggttgaag cgccaccggg cgatctcgat cagctccgcg acgaccgtct
    13021 cgggaacccg ggcgggatcg acggtgcaca cgcgtaaggt ctcgcggacc agcccttcgg
    13081 gacccaggcg ccgcgaccgg gcggcgacga ggcgctcacc cgcgaacggc atcgagtagg
    13141 ccgcgaacgt cgctacgatg agcggatcgc cgatcgttcg cggcggacgc ggcagcgcgg
    13201 gcgagaccag aaccagcgaa gcgacggtct cgggcgcggc ggccgcttgc atcagcgaga
    13261 gcaggccgcc catcgagttg cccatcagca cggcgcgacc gccggcgacc tcggtgatga
    13321 accggtgaag gatccgccgg ttgtcgtgca ccgagctggt gcggccgtgg ggcggggtcc
    13381 gtccgaatcc cggcaggtcg ggagcgagca cgcgcccgac ctgggtgaag aggggcgccg
    13441 cggccatcca gttcgcatgg ttgccgccga gaccgtggac cagcacgacc gggggtccgt
    13501 ccccgccgaa gtcggcgtag gcgacggggc cgtcgatgtc ggcttcgagg agttgcacgg
    13561 ccgaaacggt acacgctccg cgggcgattc gcgacgatga tgcaagcgat cgggcgaatc
    13621 gcatcgccat tggtcaaagc acgcgtcgaa ttccggctcg aaatgggaac attgggcgat
    13681 ccgtatcgtt caagataggg gggcagaccg ccgacgatgc aggcaggaag ccaggttctc
    13741 tacaggatga ggtctcgttc gacgatgcga aaattctcca ggggcggggt ggttctcgcc
    13801 gcacttctcg ttcagctcgg cttgttcccg accgggcccg ctcgagcggt gctggatcgt
    13861 ccggacccgg tgcgccacgt gcacaccgat ccgcccgagg cgttgccggc ggttccccgg
    13921 ccgcctcgcg cgtcggcccc gggcgaggtg agtgccgccg cgtccacttc gccggcgtgc
    13981 tcgagcacgc acagcaaggg ctaccagatg ctctacgtgt atccctcgga cgcgacgtcg
    14041 cgactgtcgt cgctgcagag cgtcttcctg gcggacgccg ccgccgcgca ggatcgcatc
    14101 gccgcggagt cggcatcggc gccgaagcgg ccacggttct tctgcgggat caccgcggtc
    14161 aagaccaacc gtccgggcgc gctctacacc gccgacccgc tgccgcagat cttggacgat
    14221 ctcgcgctcg cgggccacaa ccgcagcgac cgcaagtacc tgctgtggtg ggacggcccg
    14281 aaccacccgt tcgcctgcgg cgaaggaacc ttcgccgacg atgaccgctc gtcgccgtcg
    14341 cagaactaca acaacaccgg ccccgactgg gcggtggtgt acaagccacc gggcggcggc
    14401 tcgaacttct gcggatggac cacggtgctc cacgagatcg ggcacacgct cgggtcggtg
    14461 atgcattcgg cgccgaacgc gaccgggcag tggcactgcc gcgacgagaa cgatgtcatg
    14521 tgctacgtgg acaattccgg ccttccgacg ttcgaggcct gcccgggcac ctacatccgc
    14581 ttcgactgca acggcgacga ctacttcaac ccctcgcccg cggcgggctc gtacctggct
    14641 acgcattgga acacgtacaa ctcgtcgtgg ctcgagaccg cgcccgcgcc ctccgcgccc
    14701 gacaccacga tcaccggcgg cccgagcggc tccggcaacg cagcgtcggc gacgttcacg
    14761 ttcacctcga cgatcgcggg ctcgaccttc acctgctcgc tcgacggcgc accgggacag
    14821 tcgtgcacgt ccccgaagtc ctactccggg ctcgccgacg gctcgcacac cttcacggtg
    14881 tacgcaacgg ccaacggggt gtccgatccg actcccgcgt cccgcacctg gagcgtgggg
    14941 gcgggcggcg gttcgagccc cgttccggac acctcgatca ccgtcaagcc gtcgtcgccc
    15001 acgaaggaaa ccacggcgct gttccagttc acctcgaccg tggcgggtgc ggccttcacg
    15061 tgcacgctcg atggaggaac gccgtttgcg tgctcttccc cgcagtcgtt caccggcctg
    15121 gccgagggtc cgcacacgtt cacggttgcg gcgacggcgg gcgggcagcc cgacccgagc
    15181 cccgcctccc acgcctggac cgtcgacaca gtcgcgcccg tggtgacgct caccgcgccg
    15241 aacgtggcga tccagggcgc gcgatcgttc cgcgtcgagt gggccgtgca ggacgacagc
    15301 gaggtccccg tccagcgggt gctcgtgcga tcggcgtcca ccgcgcagca gtttggttgg
    15361 tggtctgtct ggcgcgagca gtcctcgccc ggcgcgacct tcatcggcgg agccggctgg
    15421 acctactgct tcgtgatcga ggtcttcgac gcggccggta accgtgccgt caacgtcggg
    15481 caaagctgca ccgcggtgcc gctcgacgac cgatggacgc cggcgcgcgg cttcgagcgc
    15541 cggacggaga acgggttctt cgacaactct gcgtcgatcg cccggtcgag cggcgcgacg
    15601 atgacgctgt cgaacattcg cgcgcgcgag atcgacatgc gcgtgcagat gtgccgcgcc
    15661 tgccgctaca tcgacgtcta ctggaacggg cagcgcatca agcagatgtc gctgtggacg
    15721 aacgatccgg cgatcgtgtc ggtccggctt gcgagcttct cctcgctgca gaccggcacc
    15781 ctgcagctgc gcatcgtgtc gccggtcatc gtgatcgact ccatcggcgt cagccaatat
    15841 tagaccgcga aacgacgaaa cccaccggat gacggtgggc ttcgtcgcgt tgctcgtggc
    15901 tcagccgttg ggacgcgcga cgcagcgcac ggcctcgagc tggccgcagg cgccgccggt
    15961 tgcggcgacc ggcgcgccgg gaattaccgg cagtgtgatc gaggacggac gcgacgcgtc
    16021 gtgccagagc gtgttcatcg ccgcgggaac ctgggccacg tacgagtagt acgactcgac
    16081 ctgcggtggg gccatgatct tgaccatgat cttgtgcccg ggccggaaca cgtgaccgac
    16141 ggggaagatc tcgagcaggt atcgctcgac ggtgccgggc gtgaccggct gcgggttggt
    16201 gtggggacgc cagggccggt acaggtgcgc gcccggccgg tcgcaggcgg tgcgcacgtt
    16261 cgaggtatcc acgcagtcgc tcttacccag gtcgatcgcg cggtggctcg ccttcaggat
    16321 gccgcgctgc aggtacgtgc gggcgccggc cggcgacacg tcgatgagct gggcgaacag
    16381 ggccgtatcg ggcgccgacg acgacaggta caggtcggcc gtgatcgggc cggcgatcga
    16441 cagcggcgcg gtcatctcgg ctgaggtgaa cgtcagctcg tcgcttccgt cggccgtggt
    16501 gaacggcgag ccggcggccg gccccgcctg atacgaccac gactgacggc cgctgcccga
    16561 cagatagccg ctcgatcctt cgccggccga cggcgcgctc gccgacagtc ccccgcccgc
    16621 tcggaagtag tacggcgtcc acgcggtggt gctcagcggg aagcgcgtgt cgctgatcgt
    16681 ggagcgcggc gcgttgttgt actcctgcag cacgtccacg gaagtcaggc gctgcgcgat
    16741 cgtgccgtat cccgcgtcga ttccccgcat ccagtggtcc atccacgcgc gccggtcgtt
    16801 catgacgtag gtgtcttgat tggtgccgtg gtcgccgttg aaggtcatca gccgcttcgg
    16861 aacgccagac acctgctccc acagg
//
LOCUS       DATLFO010000283         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_16113, whole genome shotgun sequence.
ACCESSION   DATLFO010000283 DATLFO010000000
VERSION     DATLFO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16113"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VM841_11525"
     CDS             <1..968
                     /locus_tag="VM841_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HVL90850.1"
                     /translation="AAARHWWAQQHGVDVDAAGLAGLPPVPVFSSGYIHMSARKALGM
                     LGLGRDAVRTFARDDAGRLDTAALRAALQSLRGAPAIIVGNAGEVNAGDFDPIEEMAD
                     LAEEFGAWLHVDGAFGLFARVTPRAHALTRGVERAHSVASDGHKWLNVPYDCGFVFVR
                     DPAALARPFAASSAAYLPAPDDPNPNYLFLGPDSSRRARSLAVWSTLLAYGRDGYRAM
                     IERHLGLARHLARRVEQEPSLELLAPAALNIVCFRFAPDGTSEDEREALNKRVGAAVL
                     EDGRVFVGTSTYQGKTVFRPAIVNWRTAEADVDALIDVLLELSKR"
     gene            complement(965..1882)
                     /locus_tag="VM841_11530"
     CDS             complement(965..1882)
                     /locus_tag="VM841_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90851.1"
                     /translation="MSEYPKLAARLGALRAAGRHAEAAALAEAQRHLHPQEAHSIDWW
                     IMSAAAAAGEHDTALRVLEESLDRGDWFAPDRMRETGDFDSLAGDERFEQALKRCGER
                     RDEALHHARPALTIQRPASPPPWPVLLALHGNGEPVASVLRHWGGTGRLLAAPVSGSV
                     SGPDRFVWAAATTGEVRRHVATLSEMGAMTSDLIIGGFDRGAYHALRLALTGAVAARG
                     AIVVSPAMNDIEEIEGSLPDAAARGLRVAFVYGTGDPRPAKAVPRVAAQLAGAGLETR
                     VESRDELVRDYAEDWAALLPDLLAFIAPA"
     gene            complement(1979..>2350)
                     /locus_tag="VM841_11535"
     CDS             complement(1979..>2350)
                     /locus_tag="VM841_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90852.1"
                     /translation="VTASGIPAVLNGIAAIDVQALAPFPLGIPAPAGHAVLTLQSTFE
                     PPKPRVVSVTLWCTALEPGPAGPVFYGSGPGSDGATWHVSVGQGEILWHVGWSTTASR
                     DAPCGGPRPVHPLIGAAAITP"
BASE COUNT          324 a          840 c          871 g          315 t
ORIGIN      
        1 tcgcggccgc gcgtcactgg tgggcgcagc agcacggggt cgacgtggac gcggccgggc
       61 tcgcgggcct gcccccggtg ccggtcttct ccagcggcta catccacatg agcgcccgca
      121 aagccctcgg catgctcggg ctggggcgag atgcggtccg aacattcgcg cgcgacgacg
      181 ccgggcggct cgacacggcc gcgttacgtg cggcgcttca atcgcttcgc ggcgcgcccg
      241 cgatcatcgt cggcaacgcg ggcgaggtga acgcgggcga tttcgatcct atcgaggaga
      301 tggccgatct tgccgaggag ttcggcgcgt ggctccacgt ggacggcgcc ttcggccttt
      361 tcgctcgcgt cacaccacgc gcgcacgcgc tgacccgcgg cgtcgagcgg gcacactcgg
      421 tcgccagcga cggacacaag tggctcaacg tgccctacga ctgtgggttc gtattcgtgc
      481 gcgacccggc cgccctggcg cggccgttcg cggccagcag cgccgcgtac ctgccggccc
      541 ccgacgatcc gaatcccaat tacctgttcc tcggcccgga ctcgtcgcgg cgcgcacggt
      601 cgctggcggt ctggtcgacg ctgctggcct acgggcgcga cggctatcgc gcgatgatcg
      661 agcgtcacct cggcctggcc cggcacctcg cgcggcgcgt ggagcaggag ccttcgctcg
      721 agctgctcgc cccggccgcg ctcaacatcg tgtgcttccg gttcgcgccg gacggcacga
      781 gcgaggacga gcgggaggcg ctcaacaagc gcgtcggcgc cgcggtgctc gaggacggac
      841 gcgtgttcgt cgggaccagc acctaccagg gcaagaccgt cttccggccg gcgatcgtca
      901 actggcgcac cgccgaggcg gacgtggatg cgctgatcga cgtgctgctc gagctgtcga
      961 agcgctaggc cggtgcgatg aaggccagga gatccggcag cagcgcggcc cagtcctcgg
     1021 cgtagtcgcg gacgagctcg tcccgggatt cgacgcgggt ctcgaggccc gcaccggcga
     1081 gctgcgcggc cacgcgcggc accgccttcg ccggacgagg gtcgccggtt ccgtagacga
     1141 acgcgacgcg caggccgcgc gcggcggcgt cgggcagcga tccctcgatc tcctcgatgt
     1201 cgttcatcgc gggcgagacc acgatcgcgc cgcgtgcggc gaccgccccg gtcaacgcga
     1261 gccggagcgc atgataggcg ccacgatcga atccgccgat gatcaggtca ctcgtcatcg
     1321 cgcccatctc ggaaagcgtc gcgacgtggc ggcgcacctc gcccgtggtc gccgccgccc
     1381 acacgaagcg gtcgggtccg gagacgcttc cggagaccgg cgccgcgagc aggcggccgg
     1441 tgccgcccca atggcgcagg acgctcgcga cgggctcgcc gttgccgtgc agggccagca
     1501 gcaccggcca cggcggcggc gacgccgggc gctggatcgt caacgcgggg cgagcgtggt
     1561 gcagcgcctc gtcgcggcgt tcgccgcagc gcttgagcgc ctgctcgaag cgctcgtcac
     1621 cggcgagcga atcgaagtcg ccggtctcgc gcatgcggtc cggcgcgaac cagtcgccgc
     1681 ggtccaagga ctcttcgagc acccgcagcg cggtgtcgtg ctcgccggcc gcggcggcgg
     1741 cggacatgat ccaccagtcg atcgaatgcg cttcctgggg atgcagatgc cgctgcgcct
     1801 cggccagcgc ggccgcttcg gcatgccgcc cggcggcgcg caaggcgccg agccgcgcgg
     1861 caagcttcgg atactcggac atggtctctc cggagaggcg aagggccggt gagtcgccgg
     1921 cccctcgcag catacgatgt gaaactcatc ggtggcggtg cccgcgcagc cacccccgtc
     1981 acggggtgat ggcagcggcg ccgatgagcg gatggaccgg gcggggtccg ccgcacgggg
     2041 cgtccctgga ggcggtcgtc gaccatccca cgtgccacag gatctcgccc tggccgacgc
     2101 tgacgtgcca ggtggcgccg tcgcttcccg ggccggatcc gtagaagacg ggcccggccg
     2161 gacccggctc gagcgcggtg caccacagcg tgaccgacac cacacgcggc ttcggtggct
     2221 cgaaggtgga ctgcaaggtc aggaccgcgt ggccggccgg cgcggggatc ccgagcggga
     2281 agggcgcgag cgcctgcacg tcgatcgcgg cgatgccgtt gagcaccgcg gggattccgg
     2341 aggcggtgac
//
LOCUS       DATLFO010000284        11412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_125049, whole genome shotgun sequence.
ACCESSION   DATLFO010000284 DATLFO010000000
VERSION     DATLFO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125049"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VM841_11540"
     CDS             <1..221
                     /locus_tag="VM841_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HVL90853.1"
                     /translation="EVRIDEPEKTALVVVPDYQLSLAIGKEGQNARLAARLTGWRVDI
                     KAESTPEAADDGGQAAAAPSATPQGGEQ"
     gene            529..2700
                     /gene="infB"
                     /locus_tag="VM841_11545"
     CDS             529..2700
                     /gene="infB"
                     /locus_tag="VM841_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HVL90854.1"
                     /translation="MSKRVYELAKEMGVTSRDVLQLLAALGVVAKSHASTVDDATATR
                     VKEAVARVMNRGAAAKPAATRPARPAARPAAQRPPAERPSAERAPRPGGNQPQQQRRP
                     GGPPQRGHQQPGRHAPAAAQQQPRAPQPARPDEPAVRKAMKIHRGATVQELAEKLSIK
                     AAEVVKKLFTMGEMATITKSLSDEALVMIGDLYNYDVDIVTPEEEEQALLGREDDDKP
                     EDLKGRPPVITVMGHVDHGKTKLLDQIRQTDVVAGEAGGITQHIGAYQVHKNGQAITF
                     IDTPGHEAFTQMRARGARITDIAVLVVAADDGVKPQTIEAISHARAAEVPIVVAVNKI
                     DKPEADPTRVRQQLTEHELVPEEWGGDSIFVDISAKEGLHIDKLLEMLLLVAEVQELK
                     ANPDARARGTVIEAHLDKGRGPVATVLVARGTLKIGDSIVCGPAWCRVRAMFDEHGRP
                     LKEVSPGSPAVVIGFSEVPIAGEDFRAVENDRTARQVAQDRVTKQRAADMVSAQKRVS
                     MQDLFGQAADGEISELNVILKGDVQGSVEAIDDALAKIKVGDIRVRVLHRAVGGITEN
                     DVSLAQASGAVVIGFNVRPSPAARDLAEREKVEIRTYTIIYKLVEEVEAALKGMLKPE
                     FAEHVLGRAEVRATFKVPKIGMIAGCYVAEGVASRNAKVRLLRDGVVLADTTVSSLKR
                     FKDDAKEVQTGYECGIGLEGYNDLKEGDHLEFYEVREVPRD"
     gene            2743..3036
                     /locus_tag="VM841_11550"
     CDS             2743..3036
                     /locus_tag="VM841_11550"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HVL90855.1"
                     /translation="MFVGSAEFSIRIPEAHSLKQKRAVLRSLTGLIKGKFDCAVAEVG
                     QQDVWQRATIGVAVVAETHFHAERLLREIERHVESHPGSDLIAAVLDVAKPGE"
     gene            3172..3990
                     /locus_tag="VM841_11555"
     CDS             3172..3990
                     /locus_tag="VM841_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVL90856.1"
                     /translation="MAGGKRAAAILTALLVIPALSGIGNAASIPSSMSALGDSITRAF
                     NAGGWYVDYPSRSWSTGTDTGVNSHYLRIRAKNSAINNKNYNDARSGAEMIDLNGQAQ
                     TAVSRGVQYVTILMGANDVCASSEAGMTSVTDFRNQFQTAMTTLTSGLPNASIFVGSV
                     PRIKRLWQVGKDSSSARAAWDGYNICQSMLANPLSTAQADVDRRNRVDQRNIDFNTQL
                     QQVCAQYANCKFDDNAIFQYKFLLSHMSTWDYFHPNKSGQAVLASVSYAAGFNW"
     gene            4079..4468
                     /gene="rbfA"
                     /locus_tag="VM841_11560"
     CDS             4079..4468
                     /gene="rbfA"
                     /locus_tag="VM841_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HVL90857.1"
                     /translation="MSNATYPRAERVRHAIQEIVAAELERMADPGLGLVTITDVTVTP
                     DLRHAKVFYTVYGSDVTEASTKDALKRATGHLRTVVAQQVRMRYVPQIEFVPDPVPQR
                     AAKIEGLLAGLRRKESDDATLADNDDD"
     gene            4481..5467
                     /locus_tag="VM841_11565"
     CDS             4481..5467
                     /locus_tag="VM841_11565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HVL90858.1"
                     /translation="MTITEAAWNEAVAAIDAAEHPVLACHLGPDGDALGSMVALGLGL
                     ASRGRKVTASWSEPFAVPRQYEWLPGLDLLAPPSDVPAEPDVMITFDAGSLERLGTLE
                     PQARAAKKLIVVDHHRSNDSFGHINLVDPDAAASAVLVHELLGRMGIPLDREIAQNLY
                     VGLVTDTGRFQYSNTTPRVMRIAADLISHDIDHPRIARIVYDTHPLGYLHLLSIALSR
                     IELIAGSSTESTMVWTWIDNDDLKTAGIGLDETEALIDVVRVAEEAEVACVLKETPDG
                     RFKVSMRAKGAIDIGAICEALGGGGHALAAGFTAEGTDPRAVALDIADRLKA"
     gene            5464..6324
                     /gene="truB"
                     /locus_tag="VM841_11570"
     CDS             5464..6324
                     /gene="truB"
                     /locus_tag="VM841_11570"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HVL90859.1"
                     /translation="MNGLLIADKPAGMTSHDAVAICRKRLAERKIGHAGTLDPDATGV
                     LVLGVGRATRLLQYLEAHDKTYTSTFVLGVETSTQDASGQVVASVDAAHVTRDDVEAV
                     LPRFRGEIDQVPPMVSAVKVGGEALYKKARRGETVERVARRVTVHELELTSFDPPAIR
                     VRCSKGTYIRTLVHDIGVALGVGAHVATLRRTAVGPYTEADATPVQDVAPESLRPMGD
                     AVAGYPRREVDAAAAVRLAHGKAIDAAGIDGPYAVFAEGALVAMARDAGTEARSLCVL
                     VHAADLAADR"
     gene            6321..7247
                     /locus_tag="VM841_11575"
     CDS             6321..7247
                     /locus_tag="VM841_11575"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HVL90860.1"
                     /translation="MNIYRSTDDIPPGIKPTAVTIGVFDGVHKGHQMLIRRVVELARE
                     TGATPGVVTFDRHPLEVVAPGKEPAMISTVEQRAEVLGGLGIEFVLVLTFDEQLRMLE
                     PHEFVRQILVDGLGVTHVVVGSNFRFGYHHAGTVATLHDLGAFEGFGVDIFSLLGGQD
                     AISSTLIRRQIAGGEVEVVAEELGRPFRLEGEVVRGAGRGKGLGVPTANLQISARMAL
                     PKLGVYAGWLTAAGRRRPAVVNVGLNPTFEDRSDPVVEVHVLDFDGDLYGKTVAIEFT
                     HRLRDELKFDGPDPLVEQIRADIAQARALLGV"
     gene            complement(7244..8491)
                     /locus_tag="VM841_11580"
     CDS             complement(7244..8491)
                     /locus_tag="VM841_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90861.1"
                     /translation="MTTKIKPSSRFARLALLAGALALFATACEYRPEKGLVKHESEGK
                     GGDHGAPEETQEPDPSSTENITDPGLGPTNPPPKPQGGYGLKLLAGSGARSYADGKGA
                     SAAFVQPIGIAVDADGNVVVADAGANRIRKVAKDGTVTTIAGSGEEGAANGAATDATF
                     RGPRGVAIGVDGTIYVADTGNHLIRTIGPNGTVATLAGSPGKKGLKNAPGAGALFNAP
                     IGIGIGPDGTLFVTEFDNHTIRVVSKDGKIVRQLAGTGVKGFKDEAALKAQFDSPVGI
                     AVDAKGVVYVADAANNRIRKIADGKVSTLAGSRAGFKDGPGKDALLNIPYGLALAEDG
                     NIYLTEFANNTVRRLDTKGAVTTIFASKDQQSVDSGTASPSPSPAKSKKEILQLPAGI
                     ASVGGKLYVANSGQSKIQIVAKS"
     gene            8637..8906
                     /gene="rpsO"
                     /locus_tag="VM841_11585"
     CDS             8637..8906
                     /gene="rpsO"
                     /locus_tag="VM841_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HVL90862.1"
                     /translation="MALQPAEKAPIIQDFRVHDRDTGSAEVQVALLTKRINDLTEHLK
                     TFKKDHHSRRGLLMLVGKRRRLLKYIASNDVAKYRALIERLGIRR"
     gene            9149..>11412
                     /locus_tag="VM841_11590"
     CDS             9149..>11412
                     /locus_tag="VM841_11590"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVL90863.1"
                     /translation="MNAPITLSREIGGRTFTLESGKLARLADGACLLTSGETTVLVTA
                     VASKKPKDHLDFFPLTVDVEERMYARGQIPGSFFRREGRPSETAILIARLTDRPLRPS
                     FPKGFRNEVHVVATILGADMENPPDVIAITGASMAVVLGGMPFEGPIAGLRLGFRQAQ
                     SIDGSMRDEWILNPTYSEIEDCIFDLVVAGSRNSEGGVDIIMVEAEATENAWTLIQEG
                     ASAPTEEIVAAGLEKAKEYIGELCDFQAEFAKMAAKPVREFPTFADYGDDMYAKVSQI
                     AEKELADAFTIADKASRDAATDEIKERVKSEILADESMLERESEIGAAFRSLTKTLVR
                     KRIVKEGLRIDGRKTDEIRPLSAEVKMLPRAHGTGLFQRGETQVLSIATLGMKRMEQM
                     IDTLDPEESKRYMHHYNFPPFSTGETGFMRGPKRREIGHGALAERALLPVVPSEEEFP
                     YTIRVVSEVLSSNGSTSMASVCGSTLSLMDAGVPIKAPVAGIAMGLIRDEGEYVTLTD
                     ILGAEDAFGDMDFKVAGTKDFITALQLDTKLTGIPSEVLTQALTQAKSARLAILDVIR
                     SAIAEPREEVAEHAPKIISVQIPVDKIGEVIGPKGKKINEIQAATGAEIDIEDDGMVF
                     IASTGGTAAAEAEKMIREIVWPVMPEIGMRFTGKVAGLTDFGAFVSIPGGKDGLVHVS
                     KLGFGRTRVAHPSDVLENGQEIEVQVMRIDERAGKISLAPIDPESGEPYEPPERERSD
                     RGDRGDRPRGGDRGR"
BASE COUNT         1934 a         3828 c         3919 g         1731 t
ORIGIN      
        1 aagaggtccg catcgacgag cccgagaaga cggccctggt ggtcgtgccc gactaccagc
       61 tctcgctcgc gatcggcaag gaagggcaga acgcccggct cgccgcgcgc ctgacgggct
      121 ggcgcgtgga catcaaagcg gagtccacgc cggaagccgc cgacgacggc gggcaggccg
      181 ccgcggcgcc ctcggcgacc ccgcagggcg gagagcagta agattggggt tcgtggccga
      241 acctgcctcg cctgccgtcg gatacgcggc aaggcgcagc tggtccggct cgttcgcggg
      301 tccgacggag cggcacgtgt cgatcggact ggcaccgcgt ccgggcgggg cgcgtatctg
      361 tgcccgtcgg acgagtgctt cgcgaacggc gtacgccggc tcgggggcgc gcttcgcggc
      421 gcgcgcgtgg accggctcac gctgaaagag gatttcgaga ccgcactcga agagcggcgg
      481 atcgagcacg acgaagcggc tggcgatgcc gcgctgggag aggattgagt gagcaagcga
      541 gtctacgagc tggcgaaaga gatgggggtc acgtcgcgtg acgtccttca gctgctcgcc
      601 gcgctcggcg tcgtggccaa gagtcacgcc tcgaccgtgg acgacgcgac cgccactcgc
      661 gtgaaagagg ccgtggcccg ggtgatgaac cggggcgccg ccgccaagcc ggccgcaacg
      721 cgtcccgcgc gtccggcggc gcgtccggct gcccagcgtc cgcccgccga gcgtccttcg
      781 gccgagcgcg cgccgcgtcc cggcgggaac cagccgcagc agcagcgccg ccccggcggg
      841 ccgccgcagc gcggccacca gcagcccggg cgtcacgcgc ccgcggccgc gcagcagcaa
      901 cctcgtgcgc cgcagcccgc gcgtcccgac gagcccgccg tgcgcaaggc gatgaagatt
      961 catcgcggcg cgacggtgca ggagctggcc gagaagctct cgatcaaagc ggccgaggtc
     1021 gtcaagaagc tattcacgat gggcgagatg gccacgatca ccaagtctct gtccgacgag
     1081 gccctggtga tgatcgggga tctctacaac tacgacgtgg acatcgtcac gcccgaggag
     1141 gaggagcagg cgctcctggg ccgcgaggac gacgacaagc ccgaggatct caaggggcgt
     1201 ccgccggtca tcaccgtgat gggccacgtc gaccacggaa agacgaagct gctcgaccag
     1261 atccgtcaga ccgacgtcgt ggccggcgag gcgggcggga tcacccagca catcggtgcg
     1321 tatcaggtcc acaagaacgg ccaggcgatc accttcatcg acacgccggg ccacgaagcc
     1381 ttcacgcaga tgcgagcgcg cggtgcgcgc atcaccgaca tcgcggtgct cgtggtcgcg
     1441 gccgacgacg gggtcaagcc gcagacgatc gaggcgatca gccacgcgcg cgcggccgag
     1501 gtgccgatcg tcgtcgcggt caacaagatc gacaagcccg aagccgaccc gacccgcgtg
     1561 cgccagcagc tcaccgagca cgagctggtg ccggaagagt ggggcggtga ctcgatcttc
     1621 gttgacatct cggccaagga aggcctgcac atcgacaagt tgctcgagat gctgctgctg
     1681 gtcgccgagg tccaagaact gaaagccaat cccgatgcgc gtgcgcgcgg aaccgtgatc
     1741 gaggcgcacc tggacaaagg tcgcggcccg gtcgctaccg tcttggtcgc gcgaggcacg
     1801 ctcaagatcg gcgactcgat cgtgtgcggt ccggcctggt gccgggtgcg cgcgatgttc
     1861 gacgagcatg gccgcccgct gaaagaggtg agcccgggat cacccgcggt cgtcatcggt
     1921 ttcagcgaag taccgatcgc cggggaggat ttccgcgccg tggagaacga ccgcaccgcg
     1981 cgccaggtcg cgcaggaccg cgtcaccaag cagcgggccg ccgacatggt ttcggcgcag
     2041 aagcgcgtgt ccatgcagga cctgttcggc caggccgccg acggcgagat cagcgagctg
     2101 aacgtgatcc tcaagggcga cgtgcagggt tcggtcgagg cgatcgacga cgcgctggcc
     2161 aagatcaagg tcggagacat tcgcgtgcgc gtgctgcatc gcgccgtcgg cggcatcacc
     2221 gaaaacgatg tttcgctcgc gcaggcctcg ggcgcggtcg tgatcggttt caacgtgcgt
     2281 ccgtctcccg cggcccgcga cctcgccgag cgcgagaagg tcgagatccg cacctacacg
     2341 atcatctaca agctcgtcga agaggtcgag gcggccctca agggcatgct caagccggag
     2401 ttcgccgagc acgtgctggg ccgcgccgag gttcgcgcga ccttcaaggt gcccaagatc
     2461 ggcatgatcg ccggttgcta cgtcgccgag ggcgtcgcca gccgcaacgc caaggtgcgg
     2521 ctgctgcgcg acggcgtggt gctggcggac accaccgtgt cgtcgctcaa gcggttcaag
     2581 gacgacgcca aggaagtgca aaccgggtac gagtgcggca tcggcctgga gggctacaac
     2641 gacctcaagg aaggcgatca cctcgagttc tacgaggtcc gcgaggtccc gcgggactag
     2701 gggagccggg ggccggcggc gcgtggcccc cgatcgaaca gaatgttcgt cggatccgcg
     2761 gagttctcga tccggattcc cgaggcccac tcgctcaagc agaagcgtgc ggtgttgcgg
     2821 tcgctgaccg gtctaatcaa gggcaagttc gactgcgcgg tggccgaggt cggccagcag
     2881 gatgtgtggc aacgcgccac gatcggcgtc gccgtcgtcg ccgagacgca ttttcacgcc
     2941 gagcggctcc tccgcgagat cgagcgccat gtcgaaagcc atcccggatc ggacctgatc
     3001 gccgcggtcc tggacgtggc caagcccggc gaatagtcac ggttgcttga tttgttcgga
     3061 acaccctgat taccatccgc ccgcccggaa cgtccgggag tggcggaccc cgtcccgctc
     3121 tctctgctcg atcggaacgg cccgtcgtat gcgaccccga ggaggcctcg catggcagga
     3181 gggaaacgcg cggcggcgat cctgaccgcg ttgctggtca ttcccgcgct cagcggcatc
     3241 ggcaacgcgg catcgatccc gtcttcgatg agcgcgctcg gcgattcgat cacgcgcgca
     3301 ttcaatgcgg gcggctggta cgtcgactac ccgtcgcgct cgtggagcac gggcaccgac
     3361 accggcgtca acagccacta ccttcggatc cgggcgaaga actcggcgat caacaacaag
     3421 aactacaacg acgcccgctc cggcgccgag atgatcgacc tcaacggcca ggcgcagacc
     3481 gcggtgagtc gcggcgtgca gtacgtgacg atcctgatgg gcgccaacga cgtgtgcgcc
     3541 tcgagcgagg ccgggatgac ctcggtgacc gacttccgaa accagttcca gacggccatg
     3601 accacgctga cgagcggcct gccgaacgcg agcatcttcg tgggctcggt cccgcggatc
     3661 aagcggctct ggcaggtcgg caaggactcg tcctcggccc gcgcggcatg ggatggctac
     3721 aacatctgcc agtcgatgct ggccaatcca ctgtcgaccg cgcaggccga cgtcgaccgg
     3781 cgcaaccgcg ttgatcagcg caacatcgac ttcaacaccc agttgcaaca ggtctgcgcc
     3841 cagtacgcga actgcaagtt cgacgacaac gccatctttc agtacaagtt cctgcttagc
     3901 cacatgtcca cctgggacta cttccatccg aacaagtccg gccaggcggt gctcgcctcc
     3961 gtgagctacg ccgccggttt caactggtag caggccggtg aggaaggggc ggtccgttcg
     4021 ccgccccttc ggccgttccg ggcaggccgc cccaagcgcg gccggatagc atggccgggt
     4081 gagcaacgcg acctatccca gggccgagcg ggtacgccat gcgatccaag agatcgtggc
     4141 ggccgagctc gagcgcatgg ccgaccccgg gctcgggctg gtgacgatca ccgacgtcac
     4201 cgtcactccc gacctgcggc acgcgaaggt cttctacacc gtctacgggt cggacgtgac
     4261 cgaggcatcc accaaggacg ccctgaagcg agcgaccggc catctgcgga ccgtcgtcgc
     4321 gcagcaggtt cgtatgcgct acgtgccgca gatagaattc gttccggatc cggttcccca
     4381 acgggccgcc aagatcgaag ggctgctcgc cggcttgcgc cgcaaggaat ccgacgacgc
     4441 gacccttgcc gacaacgacg acgactgaag gagccggtcc gtgacgatca ccgaggcagc
     4501 ctggaacgag gccgttgccg ccatcgacgc cgccgagcat cccgtgctcg cctgccatct
     4561 cggtcccgac ggggacgcgc tcggatcgat ggtcgccctc gggctcggcc tggcgagtcg
     4621 cggccgcaag gtgaccgcgt cctggagcga gccgttcgcc gtgccgcgac aatatgagtg
     4681 gctgcccggt ctggacctgc tcgccccgcc gtcggacgtt cccgccgagc cggacgtgat
     4741 gatcaccttt gacgccggat cgctcgagcg gctcggaacg ctcgagcccc aggcacgggc
     4801 ggccaagaag ctgatcgtgg tggaccatca ccgctccaac gacagcttcg gacacatcaa
     4861 cttggtcgac ccggacgcgg ccgccagcgc ggtgctcgtg cacgagctgc tcggtcggat
     4921 gggcatcccg ctcgaccgcg agatcgcgca gaacctctac gtcggcttgg tgaccgatac
     4981 cggacgcttc caatactcga acacgacccc gcgcgtgatg cgtatcgcgg cggacctgat
     5041 ctctcacgac atcgaccatc cgcgcatcgc gcgcatcgtc tacgacacgc atccgctcgg
     5101 ctaccttcac ctgctctcga tcgcgctgtc gcgcatcgag ctgatcgccg gatcgtccac
     5161 ggagtccacg atggtctgga cgtggatcga caacgacgac ctcaagaccg ccgggatcgg
     5221 cctcgacgag acggaggcgc tcatcgacgt ggtgcgcgtc gcagaggagg ccgaggtcgc
     5281 gtgcgtgctc aaagaaacgc ccgacggccg gttcaaggta tcgatgcgcg cgaagggagc
     5341 gatcgacatc ggcgcgatct gcgaggcgct cggcggcggc gggcatgcgc tcgcggccgg
     5401 cttcaccgcc gagggcaccg acccgcgtgc cgtcgcgctg gatatcgcag accgcctgaa
     5461 ggcgtgaacg gcctgctgat cgccgacaag ccggcgggca tgacatcaca cgacgccgtc
     5521 gcgatctgcc gcaagcggct tgcggagcga aagatcggtc atgccggaac cctcgatccc
     5581 gacgcgaccg gcgttctcgt gctcggtgtc ggccgcgcga cgcggctgct gcaatatctc
     5641 gaggcgcacg acaagaccta cacctcgacc ttcgtgctcg gcgtcgagac cagcacgcag
     5701 gatgcgagcg gccaggtcgt cgcgtccgtg gacgccgccc acgtgacccg cgacgacgtg
     5761 gaggcggtcc tgccgcgctt tcgtggcgag atcgaccagg ttccgccgat ggtgagcgcc
     5821 gtcaaggtcg ggggagaggc cctttacaag aaagcccggc gcggcgagac cgtcgagcgc
     5881 gtggcccggc gcgtgaccgt gcacgagctc gagctcacgt ccttcgatcc gccggcgatc
     5941 cgggtgcgat gcagcaaggg cacgtatatc cgaacgctcg tgcacgacat cggtgtggcg
     6001 ctcggcgtcg gcgcgcacgt cgcgacgttg cgccgcaccg cggtcgggcc gtacaccgaa
     6061 gccgacgcca ccccggttca ggacgtggcc cccgagtcgc tccggccgat gggcgacgcg
     6121 gtcgccggct atccgcgccg ggaggtggac gcggccgcgg ccgtgcggct ggcgcacgga
     6181 aaggccatcg acgccgccgg catcgacggc ccgtacgcgg tcttcgccga aggcgccttg
     6241 gtcgcgatgg cgcgcgacgc cggcaccgaa gcgcgtagcc tatgcgtcct cgtccatgcc
     6301 gccgacctcg ccgcagaccg atgaacatct accgctcgac cgacgacatc ccgcccggca
     6361 tcaagccaac cgccgtcact atcggcgtct tcgacggcgt ccacaaggga caccagatgc
     6421 tcatccggcg cgtcgtcgag ctggcacggg agaccggcgc gactcccggc gtggtcacct
     6481 tcgaccggca tccgctcgag gtcgtcgcgc ccggcaagga gcccgcgatg atctcgacgg
     6541 tcgagcagcg ggcggaggtg ctcggcggcc tgggcatcga gttcgtcctc gtgctcacct
     6601 tcgacgagca gcttcgcatg ctcgagccgc acgagttcgt ccggcagatc ctggtggacg
     6661 gactgggcgt gacccacgtc gtcgtgggca gcaacttccg cttcggctac caccacgccg
     6721 ggaccgtcgc aacccttcac gatctcggcg cgttcgaagg cttcggcgtg gacatcttct
     6781 cgctgctcgg cggacaggac gcgatttcgt ccaccttgat ccgccgccag atcgcgggcg
     6841 gagaggtcga ggtcgtcgcc gaagagctgg ggcggccctt ccgcctggaa ggagaagtcg
     6901 ttcggggagc gggtcgcggc aaggggctgg gcgttccgac tgcgaacctg cagatctcgg
     6961 cgcgcatggc cttgcccaag ctcggcgtct acgcgggctg gctcaccgcc gccggccggc
     7021 gccggccggc ggtggtcaac gtcgggctca atccgacctt cgaggaccga agcgatcccg
     7081 tggtcgaggt tcacgttctg gacttcgacg gcgacctgta cggaaagacg gtcgcgatcg
     7141 agttcaccca ccgtctgcgc gacgagctga agttcgacgg cccggacccg ctcgtcgagc
     7201 agatccgggc cgatatcgcg caggcgcgcg ccctgctggg cgtctagctc ttcgcgacga
     7261 tctgaatctt ggactggccg gagttggcca cgtacagctt tccgccgacg gaggcgatgc
     7321 ccgccggaag ctgcagaatc tctttcttgc tcttggcggg cgacggcgac ggcgacgcgg
     7381 tccccgagtc caccgactgc tgatccttgg aggcgaagat cgtggtcacc gcgcctttgg
     7441 tgtcgagccg gcggaccgtg ttgttggcga actccgtgag gtagatgttg ccgtcctcgg
     7501 cgagcgcgag cccgtaggga atgttgagca gcgcatcctt gccgggtccg tccttgaagc
     7561 cggcccggga gccggcgagc gtgctcacct tgccgtcggc gatcttgcgg atgcggttgt
     7621 tggccgcgtc ggccacgtag accacgccct tggcgtccac cgcgatgccg acgggcgagt
     7681 cgaactgcgc cttgagggcc gcctcgtcct tgaatccctt gacgccggtg ccggcgagct
     7741 ggcggacgat cttgccgtcc ttggacacga cccggatcgt gtgattgtcg aactcggtca
     7801 cgaacagggt gccgtccggg ccgatgccga ttccgatcgg ggcgttgaac agcgcgccgg
     7861 cgccgggcgc gttcttgagg cccttctttc cgggggatcc ggccagcgtg gccacggtgc
     7921 cgttcgggcc gatggtgcgg atgagatggt tgccggtgtc ggcgacgtag atcgtcccgt
     7981 cgactccgat cgccacgccg cggggaccgc ggaaggtggc gtcggtcgcg gcgccgttgg
     8041 ccgcgccttc ctctccgctg ccggcgatgg tggtcacggt gccgtccttg gcgaccttgc
     8101 ggatccgatt ggcgcccgcg tccgcgacga cgacgttgcc gtcggcgtcc accgcgatgc
     8161 cgatcggctg aacgaacgcg gccgaggctc ccttgccgtc agcgtaggag cgggctccgc
     8221 tgccggcgag cagcttgagc ccgtacccgc cttgcggctt cggcggaggg ttggtcgggc
     8281 cgagcccggg atcggtgatg ttttcggtcg aggacgggtc gggctcttgg gtctcctcgg
     8341 gagcgccgtg gtccccgccc ttgccctcgg attcatgctt gaccaggccc ttctcgggac
     8401 ggtactcgca tgcagtggcg aacagcgcca aggccccggc aagcagagcg aggcgcgcga
     8461 aacggctgga tggcttgatt tttgtcgtca tggcgtgggc agaatacccc tctatccggc
     8521 ggtccgctgg cggggccatg tcgtccgccg accgtgggcc ggcgaggcga gcaagcggcc
     8581 ggagcgactg ccgtgtggct tgcggctagt gcctctggta gcattcccgc cgacctatgg
     8641 cactccaacc cgccgagaag gcacccatca tccaggactt ccgcgtccac gaccgggaca
     8701 ccggctcggc ggaggtgcag gtcgcgctgc tcaccaagcg catcaacgac ctgacggagc
     8761 atctcaagac cttcaagaag gaccatcaca gccgccgcgg cctgctcatg ctggtcggaa
     8821 agaggcgccg ccttctgaag tacatcgcgt cgaacgacgt ggccaagtac cgagcgctta
     8881 tcgagcgtct cggaatccgc cggtaaagcc ctcgccaggg ggcgtcgaca gccggccacc
     8941 cccacggggt tgcccggctc gacaactgca taccggtata actccggaag caacgacggt
     9001 cccgccaggg tcggtcagtt ttcggtcgcg agtcccaagc tcatctcttg gggccctcaa
     9061 ctgagaactg gcccgcacct cctgacccgg ccgtcggcgc aaccggctcg aggaagcgaa
     9121 gcgcatgggg cgcttcgagg aggttcacat gaacgcaccg atcacgcttt cgcgtgagat
     9181 cggcgggcgt accttcacgc tggagagcgg caagctcgct cgtctggccg acggagcctg
     9241 tctgctcacc agcggcgaga cgaccgtcct ggtcaccgcc gtcgcatcca agaagcccaa
     9301 ggaccacctg gacttcttcc cgttgaccgt cgacgtcgaa gagcggatgt acgcgcgggg
     9361 tcagatcccc ggctcgttct tccgccgcga aggccgtccg tccgagaccg cgatcctgat
     9421 cgcgcgcctc accgaccggc cgcttcgccc gtcgttcccg aagggcttcc gcaacgaggt
     9481 ccacgtcgtc gccacgatcc tgggcgccga catggagaac ccgccggacg tcatcgccat
     9541 cacgggcgcc tcgatggcgg tcgtgctcgg cggcatgccg ttcgaagggc cgatcgccgg
     9601 cctgcggctg ggcttccgcc aggcgcagag catcgacggt tcgatgcgag acgagtggat
     9661 cctgaacccg acctacagcg agatcgaaga ctgcatcttc gacttggtcg tcgccggatc
     9721 gcgtaacagc gaaggcggcg tggacatcat catggtcgag gccgaggcga ccgagaacgc
     9781 gtggacgctc atccaggaag gcgcgtccgc accgaccgag gagatcgtcg ccgccgggct
     9841 cgagaaggcc aaggagtaca tcggcgagct gtgcgacttc caggccgagt tcgccaagat
     9901 ggcggccaag cctgtgcgcg agttcccgac cttcgccgat tacggcgacg acatgtacgc
     9961 caaggtctcg cagatcgccg agaaagagct cgccgacgca ttcacgatcg ccgacaaggc
    10021 gtcgcgtgac gcggccaccg acgagatcaa ggagcgcgtc aagtccgaga tcctcgcgga
    10081 cgagtccatg ctcgagcgcg aaagcgagat cggagcggca ttccgttcgc tgaccaagac
    10141 gctggtgcgc aagcgcatcg tcaaggaagg ccttcgcatc gacggccgca agaccgacga
    10201 gatccggccg ctatcggcgg aagtgaagat gcttccccgg gcgcacggca ccggattgtt
    10261 ccagcgtggc gagacgcagg ttctgtcgat cgcgacgctg ggcatgaagc gcatggagca
    10321 gatgatcgac acgctcgatc cggaagagtc caagcgctac atgcatcact acaacttccc
    10381 gcccttctcg acgggtgaga ccggcttcat gcgcggtccc aagcgtcgcg agatcggcca
    10441 cggcgcgctc gccgagcgtg ccctgctgcc ggtcgtgccg agcgaggaag agttcccgta
    10501 cacgattcgc gtggtgtccg aggttctgtc gtcgaacgga tcgacgtcga tggcgtcggt
    10561 gtgcggctcg acgctatcgc tgatggacgc gggcgtgccg atcaaggcgc cggtcgcggg
    10621 catcgcgatg ggtctgatcc gcgacgaagg cgagtatgtg acgctgaccg acatcctcgg
    10681 agcggaagac gcgttcggtg acatggactt caaggtcgcc ggcacgaagg acttcatcac
    10741 ggcgctgcag ttggacacga agctgaccgg catcccgtcc gaggttctga cgcaggcgct
    10801 tacgcaggcc aagtcggcgc gtcttgcgat cctcgacgtg atccgctcgg cgatcgccga
    10861 gccgcgtgaa gaggtcgccg agcacgcgcc gaagatcatc tcggtgcaga tcccggtgga
    10921 caagatcggc gaggtcatcg gcccgaaggg caagaagatc aacgagatcc aggccgccac
    10981 cggtgccgag atcgacatcg aggacgacgg catggtgttc atcgcctcga cgggcggcac
    11041 ggctgcggcc gaggccgaga agatgatccg cgagatcgtc tggccggtca tgcccgagat
    11101 cgggatgcgc tttaccggca aggtcgccgg gctcaccgac ttcggtgcgt tcgtgtcgat
    11161 ccccggcggc aaggacggcc tggtgcacgt gtcgaagctg ggcttcggtc gcacgcgcgt
    11221 cgcgcatccg tccgacgtgc tggaaaacgg gcaggagatc gaggtccagg tcatgcggat
    11281 cgacgagcgg gcgggcaaga tctcgctcgc gccgatcgat cccgaatcgg gcgagccgta
    11341 cgagcccccg gagcgggagc gcagcgaccg cggtgaccgc ggcgaccgcc cgcgtggcgg
    11401 cgaccgtgga cg
//
LOCUS       DATLFO010000285         6838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_76513, whole genome shotgun sequence.
ACCESSION   DATLFO010000285 DATLFO010000000
VERSION     DATLFO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76513"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VM841_11595"
     CDS             complement(<1..372)
                     /locus_tag="VM841_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90864.1"
                     /translation="MSVRPSGIVHWLARAVAPAIRDSLIAALSLWLALELRFDLDVPP
                     PFSDRWYWLVPLTAGLLVLVGAVRGIYRTVPEFAGIVELIRLGEAVAAASIGSLLAAK
                     YALPDSHVVPLSVPPIAAMIMF"
     gene            620..2152
                     /locus_tag="VM841_11600"
     CDS             620..2152
                     /locus_tag="VM841_11600"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HVL90865.1"
                     /translation="MELRDYIRIIRRRRWLIALVFAACSALALLYSSSRPPVYVANAK
                     VFIGPQTIDRTDAAAAMQALTFSRDFLASYAELIRGRAFAERVVEELQLRVTPEKLTE
                     RISANVLQDTRIIQVKVSDASRERSARIANTLTELFVEDIQADFAGGSGAVQASVIEP
                     AIPPSKRSAPAPVRDGILGAFLGLMIGVGGALLLEQLDTRLRTREDLERALEEVPVLA
                     TVPRMSGEMARERRFAVDSDPRGLVAEAFRILRTNVQFLAVDRPIRRVLVTGALAGDG
                     KTTVSANLAAALATAGYTTLLVETDLRRPTVHDYIAGSPSPGISDVLLGRVRLDQAIR
                     PSGVPGLSVLTSGALPPNPSELLGSQRMVEIVEEASERFDVLVLDTPPTLPVTDSVVL
                     APRTDGVILVVRAGESQRDAVRAAASQFERVGVRVLGTVFNAASAEASGYGYQHYYDE
                     AYGSEPRRASRGLRRAKESTPFETSVPPPSEWGSATPTATNGNATHPPLRVERSPSPP
                     DA"
     gene            2145..3134
                     /locus_tag="VM841_11605"
     CDS             2145..3134
                     /locus_tag="VM841_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HVL90866.1"
                     /translation="MPKKHSLRRILGALVLIAAGMSPFGAYIALKATEPDPTPAWPSN
                     VAVITAENGSLASIAMIHFLAPPTPIIVRFPIETSLNVPGAGSLRAVTAYGIGGAPML
                     ARGLQQTAGVATPFWALWSRGSAAGRPPDSSNLGELAADVVAIIANPSTPVTTAPGTI
                     EVRSGVAYYELDRERLDGELDGTAVAPPTTAPPVSPSPSAAPLSPAATSVEVLNQGGP
                     AGAASAAADKLKAAGYTDVRVGNSPRRDIDGTVVYYKTSREAGDAVARVLGSSVTQVT
                     RIPSSIDTIAEVLVLVGREPRRAVSSPRTAPRPNTPPPSPSPSPSPSDDVIVE"
     gene            3172..5118
                     /locus_tag="VM841_11610"
     CDS             3172..5118
                     /locus_tag="VM841_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HVL90867.1"
                     /translation="MEAQTRRERRRRIRRHRTIRRAVLLGLGALIAVAGGFAWEAMSV
                     RASLGRAEPLLSSAKSAAAAADLEAAERGLRAAHEHVQAAVRRARGPFTTLAGLVPVA
                     GRTPRAIRGAAEGAGLAVRAGLRATEAIGAFPTDEDGKATFGFFQGRIPVESWRAAAG
                     PVAAADVLAGRAAARVADTPRRWVLPPVRRALTRLERDLATLREATDHATALATVIPA
                     LAGGDAAKRYLVALQNPSELRGTGGLLGGVTVLEAREGAIELTGERPDNEFGQLEKAA
                     MEMPEWFVTRYDRFDGRTLWSNVNLEPDFPTTAPLVAAYYREITGETVDGVIALDPYA
                     LSALLEVTGPVTGPHGVTFEAATVAKYVMNDVYSIYETDSLLRKAVMLEAASVIFDRI
                     KRGASPAALAEALAKSARRKRIQVWMRDEAVQRAVASLGVDGSFRPPSKNATMIGVVT
                     QNAGANKIDFYLRRAMELDVHLRDGGGARVIVKVALTNTAPTFGLPRDVIGPAPVPNR
                     PGVYEVGENTTYLSLYLPVGATDVLWREEGRLQPAERNLAPHAVISSRYVEIPSKSTV
                     TNRVAFDLPPGVWKPGRGPALWLPRQAVLEGDRWTVRVHAPPGRRIDGTKTFAWSGSL
                     NRDFLLELDMRRTVWRRIKDLVWR"
     gene            5202..5684
                     /locus_tag="VM841_11615"
     CDS             5202..5684
                     /locus_tag="VM841_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90868.1"
                     /translation="MKKLLGTVASLALALALPASTAWAQQYPTSNFVSMDDTTVVINQ
                     LVNSSACCFQGPVTWTMRSHPRVVGTSFPDTSGVARLTWRVPADAELGSHTLSASGTD
                     INGIPLTVTTTFTVVRGTGRGGLTPTGSNTIPWLLIGLGSVAVGGTLVTVAKRRGIAA
                     "
     gene            complement(5744..6664)
                     /locus_tag="VM841_11620"
     CDS             complement(5744..6664)
                     /locus_tag="VM841_11620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL90869.1"
                     /translation="MKVLVTGVAGFIGSRLAGRLIADGHEVRGLDSFLTGFKENIPDG
                     CDFLEADLRDLDAMHAATRGVEVVFHQAARRSVAKSVDDPRLSHECNATGTLNLLIAA
                     HGAGVRKFVYASSSSVYGDPPEPLRTETQVPRPISPYAVSKLAGEHYGQAFTAIHGLP
                     VVSLRYFNVFGPGQHRESKYALLFPAFVEALVEKRAPEVDWDGHQSRDFTFIDDIVEA
                     NLKAANAGPEADGESFNIGAGHGRTVNEVLEAVSDAVGRWIDPVATPKRPGDVRHTLA
                     DIGKAKRLLGWEPRADWDASVRACVDWFAR"
BASE COUNT          994 a         2468 c         2418 g          958 t
ORIGIN      
        1 gaacatgatc atcgcggcga tcggcggcac cgacagcggc acgacatggg agtcgggcag
       61 cgcatacttc gcggcgagca gcgatccgat gctcgccgcg gcgacggcct ccccgagccg
      121 gatcagctcg acgatgccgg cgaactcggg aaccgtgcga tagatcccgc ggaccgcgcc
      181 gacgagcacc agcaggccgg cggtcagcgg cacgagccaa taccagcggt cgctgaaggg
      241 cgggggcacg tcgagatcga agcggagctc gagcgcgagc cagaggctta gggctgcgat
      301 cagcgagtcg cgaatcgcgg gggcgacggc gcgcgcgagc cagtggacga tgccagacgg
      361 gcggacgctc acccttacga ccactccccc tccggcgcga gccggaatcg aagaacaaaa
      421 acgggccgga cgacctgcga caacctcctc aggccccggc cccccacgta acgcgatcag
      481 ggtatgggcg acattccaac cggtcaagca aggacgaatc ggggcaccta cggcaccccg
      541 acccgctgct ttatactttc ggaaccgtcg gggctcggcc ctcgatgtga ctcatcactc
      601 tcatcactaa ggagtccctc tggagcttcg cgactacatc cgcatcatcc ggcggcggcg
      661 ctggctgatc gcgctcgtct tcgctgcttg ctcggcgctc gccttgctct actcgtcgtc
      721 gcggccgccg gtgtacgtgg cgaacgccaa ggtcttcatc gggccgcaga cgatcgaccg
      781 gaccgacgcg gccgcggcga tgcaggcgct gaccttctcc cgcgacttcc tggcgtccta
      841 cgccgagctg atccgcggcc gcgccttcgc cgagcgggtc gtcgaagagc tgcagctgcg
      901 tgtcacgccc gaaaagctca ccgaacgcat ctcggcgaac gtgctccagg acacgcgcat
      961 catccaggtg aaggtctcgg acgcgtcccg cgagcggtcc gctcgcatcg cgaacacgct
     1021 gacggagctg ttcgtcgagg acatccaggc ggacttcgcc ggcggatccg gtgcggttca
     1081 ggcctcggtc atcgagccgg cgatcccgcc gtcgaagcgc agcgcgcccg cccccgtccg
     1141 tgacggcatc ctcggcgcgt tcctcgggct gatgatcggc gtcggcggtg cgttgctgct
     1201 cgagcagctc gacacgcggc ttcgcacccg cgaggacctc gagcgggcgc tggaggaggt
     1261 tccggtgctc gcgacggtgc cgcgcatgtc cggcgagatg gcgcgcgagc ggcgcttcgc
     1321 ggtggactcg gacccgcggg gacttgtcgc cgaagccttc cggatcctgc ggaccaacgt
     1381 tcagttcctg gccgtggacc gtccgatccg ccgcgtgctg gtcacgggag cgctcgccgg
     1441 cgacggaaag acgacggtga gcgcgaacct ggccgcggcg ctggccacgg ccggctacac
     1501 gacgctgctg gtcgagaccg accttcgccg cccgacggtt cacgactaca tcgccggaag
     1561 cccgtcaccg ggcatcagcg acgtgctgct gggtcgcgtc cggctcgacc aggcgatccg
     1621 gccctcggga gttccgggtc tttccgtgct gacctcgggc gcgctgccgc cgaacccgtc
     1681 ggagctgctc ggcagccagc gcatggtcga gatcgtcgag gaggcgagcg agcggttcga
     1741 cgtgctcgtg ctcgacaccc ctccgacgct gccggtgacc gactcggtgg tgctcgctcc
     1801 gcgcaccgac ggcgtgatcc tggtcgtgcg cgccggcgag tcgcagcgcg acgcggtccg
     1861 ggccgccgcc tcgcagttcg agcgcgtcgg cgtgcgcgta ctcggcacgg tctttaacgc
     1921 cgcctccgcg gaagcgagtg gctacggcta ccagcactac tacgacgagg cttacggctc
     1981 agagccgcgc cgcgcgtcgc gcgggctgcg acgcgccaag gagagcacgc cgttcgagac
     2041 ctcggtcccg ccgccgagcg agtggggatc ggcgacgccg acggcgacca acggaaacgc
     2101 cacgcatccc ccgctgcgcg tcgaacggtc cccgtccccg cccgatgcct aagaagcatt
     2161 cgctgcgtcg catcctcggc gcgctcgtgt tgatcgcggc gggcatgtcc ccgttcggcg
     2221 cctacatcgc gctcaaagcg accgagccgg acccgacccc ggcctggcca tccaacgtgg
     2281 cggtgatcac cgccgagaac ggctcgctgg ccagcatcgc catgatccac ttcctcgcgc
     2341 cgccgacccc gatcatcgtg cgcttcccga tcgagacgtc gctgaacgtg ccgggcgcgg
     2401 gctcgctgcg cgccgtcacc gcctacggga tcggcggcgc gccgatgctg gcgcgcggcc
     2461 tgcagcagac ggccggcgtt gcgactccgt tctgggccct gtggtcgcgg ggctcggccg
     2521 cgggccggcc gccggactcg tcgaacctgg gcgagctcgc ggccgacgtg gtcgcgatca
     2581 tcgccaaccc gtccacgccg gtcacgaccg cgcccggaac gatcgaagtg cgcagcggcg
     2641 tcgcctacta cgagctggac cgcgagcggc tcgacgggga gctcgacggc acggccgtcg
     2701 cgccgcccac gaccgccccg ccggtgagcc cgtcgccgtc cgccgcgccg ctgagcccgg
     2761 ccgcgacctc ggtcgaggtg ctcaaccaag gcggccccgc gggcgccgcg agcgcggccg
     2821 ccgacaagct gaaagcggcc ggctacaccg acgtgcgcgt cggcaactcg ccgcggcgag
     2881 acatcgacgg cacggtcgtc tactacaaga cctcgcgcga ggccggcgat gccgtcgcgc
     2941 gcgtgctcgg ctcgtcggtc acgcaggtca cccggattcc gtcctcgatc gacacgatcg
     3001 ccgaggtgct cgtcctggtc ggccgcgagc ctcgccgcgc cgtctcttcg ccgcgcaccg
     3061 cgccgagacc gaacaccccg ccgccttccc cgagcccctc gccctcgccc agcgacgacg
     3121 tcatcgtcga gtagcctcgg ccgccgcagg tcgctgatag catcggcgcc ggtggaggcc
     3181 cagacgcggc gcgagcgacg gagaaggatc cggcggcacc gaacgatccg ccgcgccgtg
     3241 ctgctcggcc tcggggcgct gatcgccgtc gcgggcggct tcgcctggga ggccatgtcg
     3301 gtgcgcgcct cgctggggcg cgccgagccg ttgctgtcgt cggcgaagtc ggccgcggcc
     3361 gccgcggacc tggaggccgc cgagcgcggc ctgcgcgccg cccacgagca cgtgcaggcc
     3421 gcggtgcgcc gcgcgcgcgg accgttcacc acgctcgccg ggctcgtgcc ggtcgcgggc
     3481 cgcacgccgc gcgcgatccg cggcgcggcc gaaggcgccg gcctggcggt ccgcgccggc
     3541 ctgcgcgcga cagaggcgat cggcgccttc ccgaccgacg aggacggcaa ggcgacgttc
     3601 ggcttcttcc agggccgcat cccggtcgag tcctggcgcg cggccgcggg accggtcgcg
     3661 gcggccgacg tgctcgccgg ccgcgccgcc gcgcgcgtcg cggacacgcc gcggcgatgg
     3721 gtcctgccgc cggttcgccg cgcgctgacg cggctcgagc gcgacctggc gacgctgcgc
     3781 gaggcgaccg atcacgcgac cgcgctcgcg accgtgatcc cggcgctcgc cggcggcgac
     3841 gcggccaagc gctacctggt cgcgctgcag aacccgtccg agcttcgcgg caccggcggg
     3901 ctgctcggcg gcgtgaccgt gctggaggcg cgcgaaggcg cgatcgagct gaccggcgag
     3961 cggcccgaca acgagttcgg ccagctcgaa aaggcggcga tggagatgcc cgagtggttc
     4021 gtgacgcgct acgaccgctt cgacggccgc acgttgtggt cgaacgtcaa cctggagccg
     4081 gactttccga ccaccgcgcc gctcgtcgcc gcctactacc gcgagatcac cggcgagacc
     4141 gtggacggcg tgatcgcgct ggatccctac gcgctgtcgg cgctgctgga ggtcaccggg
     4201 ccggtcaccg gcccgcacgg cgtgaccttc gaggccgcga cggtcgccaa gtacgtgatg
     4261 aacgatgtct actcgatcta cgagaccgac tcgctgctgc gaaaggcggt gatgctggag
     4321 gccgcgtcgg tgatcttcga ccggatcaag cgcggtgcga gccccgcggc gctcgccgaa
     4381 gcgctcgcga agtcggcgag gcgaaagcgc atccaggtct ggatgcgcga cgaggcggtg
     4441 cagcgcgcgg tggcctcgct cggcgtggac ggatcgttcc ggccgccctc gaagaacgcg
     4501 accatgatcg gcgtggtgac gcagaacgcg ggcgcgaaca agatcgactt ctatctgcgg
     4561 cgcgcgatgg agctggacgt gcatctgcgc gacggcggcg gcgcgcgcgt gatcgtcaag
     4621 gtcgcgctca ccaacaccgc gcccaccttt gggctgccgc gcgacgtgat cgggccggcc
     4681 ccggtgccga accggcccgg cgtctacgag gtcggcgaga acaccacgta cctgagcctg
     4741 tacctgccgg tgggcgcgac cgatgtgctc tggcgcgagg aggggcggct gcagccggcc
     4801 gagcgcaacc tcgcgccgca cgcggtgatc agctcgcgct acgtcgagat cccgtccaag
     4861 tcaacggtca ccaaccgggt ggccttcgac ctgccgccgg gagtgtggaa gccggggcga
     4921 ggccccgcgc tgtggctgcc gcggcaggcg gtgctggagg gggaccgctg gacggtgcgg
     4981 gtgcacgcgc caccgggccg gcggatcgac ggcaccaaga cctttgcctg gagcggctcg
     5041 cttaatcgtg actttctgct cgaactggac atgcggcgga ccgtgtggcg gcgaattaag
     5101 gatctggtgt ggcgttgacg acatccacac cagggtagac aagaaggtgc gtaccggtat
     5161 cattctcaac actggtcact agctttacgg gggaaccgga catgaagaag ctgctcggaa
     5221 cggtcgcgag cttggcgctc gccttggctc tgcccgcatc gacggcttgg gcacagcagt
     5281 atcccacctc aaacttcgtc tcgatggacg acaccacggt cgtcatcaac cagctggtca
     5341 actcgagcgc gtgctgcttc cagggtccgg tcacctggac gatgcgctcg catccgcgcg
     5401 tcgtgggcac cagcttcccc gacacctcgg gcgtcgcgcg cctgacctgg cgcgtcccgg
     5461 ccgacgccga gctcggctcg cacacgctca gcgcgtccgg cacggatatc aacggcatcc
     5521 cgcttacggt gaccacgacc ttcacggtcg tgcgcggcac cggccgcggc gggctgacgc
     5581 cgaccggctc gaacacgatc ccgtggcttc tgatcggcct gggcagcgtc gcggtcggcg
     5641 gcacgctggt gaccgtcgcc aagcgccgcg gcatcgccgc gtagagcgcc tccgaaacgt
     5701 cagagccccg cggctgccgc ggggctcttt cttttcttct tgtctagcgg gcgaaccagt
     5761 ccacgcacgc gcgtacgctc gcgtcccagt cggcgcgcgg ctcccagccc agcagacgtt
     5821 tcgccttgcc gatgtcggcg agcgtgtggc gcacgtcgcc gggtcgcttc ggcgtggcga
     5881 cgggatcgat ccatcgcccg accgcgtcgc tgacggcctc gagcacctcg ttgacggtgc
     5941 ggccgtggcc cgcgccgatg ttgaaggatt cgccgtcggc ctcagggccg gcgttcgcgg
     6001 ctttgaggtt cgcctcgacg atgtcgtcga tgaaggtgaa gtcgcgggac tggtggccgt
     6061 cccagtccac ctcgggcgcg cgtttctcga cgagcgcctc gacgaaggcg gggaacaaca
     6121 gcgcgtactt cgactcgcga tgctgtcccg ggccgaacac gttgaagtag cgaaggctta
     6181 cgacgggaag gccgtggatc gcggtaaacg cctgcccgta gtgctcgcca gcgagcttgc
     6241 tgaccgcgta cgggctgatc ggccggggaa cctgggtctc ggtgcggagc ggctcgggcg
     6301 ggtcgccgta gacgctcgac gaggacgcgt agacgaactt gcggacgccg gcgccgtgcg
     6361 cggcgatcaa caggttgagc gtgccggtcg cgttgcactc gtgcgacagg cgcggatcgt
     6421 ccaccgactt ggcgacgctg cgccgcgcgg cctggtggaa cacgacttcg acgccgcgtg
     6481 tcgcggcgtg catcgcatcc aggtcccgca ggtccgcctc gaggaagtcg catccgtcgg
     6541 ggatgttctc cttgaagccc gtcaggaagc tgtcgaggcc gcgaacctcg tgcccgtccg
     6601 cgatcagacg gcccgccagc cgcgatccga tgaagcccgc cacgccggtc accagaacct
     6661 tcatgacctt caccctatcg cgagcccaac gggaataacc gccgcgagcg ccgcgagcgc
     6721 cgcgagcgcc gcgagcgccg cgagcgccgc gagcgccgcg agcgccgcga gcgccgggag
     6781 cgccgggagc gccgggagcg aagcgtggcc ccagcggcgc agatcgaccg gcaacccg
//
LOCUS       DATLFO010000286         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_37508, whole genome shotgun sequence.
ACCESSION   DATLFO010000286 DATLFO010000000
VERSION     DATLFO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37508"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1011
                     /locus_tag="VM841_11625"
     CDS             121..1011
                     /locus_tag="VM841_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVL90870.1"
                     /translation="MARAVTAATWSRGLRMLALPLIVAAVVLVAIPAAMTILFAFTDY
                     DAVSAPRWNGLDNFARMWRDPIFATSLWNSLIFVVIAVPLRLFAAVGTALLLSGRRRG
                     TATGRALVYLPTVVPDIAYALLWLWVFNPVYGPLSMVLRAVGLPGPELLVSAWGARFA
                     VAAMTAFQIGEGFIVALAVRNDIPHELYELAEIDGARPSWTLRRVTLPLMMPALLLLA
                     ARDVVLSFQANFVPALVLTGGGPEYATTFLPLYTYQNAFEFLRFGYASAMTLTMFVVT
                     AAMIGIQFLVVRRRREGWSA"
     gene            1008..1289
                     /locus_tag="VM841_11630"
     CDS             1008..1289
                     /locus_tag="VM841_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HVL90871.1"
                     /translation="MTSWERFKAVLNFIGRSSKRIAVTAVGLTVLAAGIAMLALPGPG
                     LLVIILGLAILASEYVWARRALDMAKRKANQAKKKAGGMFRRNNKKSSV"
     gene            complement(1286..1822)
                     /locus_tag="VM841_11635"
     CDS             complement(1286..1822)
                     /locus_tag="VM841_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90872.1"
                     /translation="MSSRARFAAIALLLVIGGAVAFAASRGGDGRPAAVPSTPATLTG
                     NCLQPQPEIARPGWYPDDLPMPEGSYAIDVPPAAGGLRRVVFSVSGDLTDFVRHALGV
                     WPKHGWSLGVGESEPGEAEDNFLKGERYGIFRARSIMCDQSRTWVLMVLNDPAIKDAP
                     TPGFNTNPGTQPSPLKTD"
     gene            2007..>2636
                     /locus_tag="VM841_11640"
     CDS             2007..>2636
                     /locus_tag="VM841_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90873.1"
                     /translation="MRERSERRPVIALAAAAAVLAVAGIMPAGAQEPLTAGGFAGAAD
                     GSFPARLAAEHPMAAEYPLTWAHPSGEPITVTQPDGRRIQVIGTDMNVGGVSETVDGY
                     SVMRDPASGWWVYALKGENGWEVPTERRVGIDSSGGIPKHAARTKSVWIDGRGKDARE
                     QIFEALRQNTIQLQATAAAAAAGGATVFKIPALMLETHGPFADESTPDFF"
BASE COUNT          377 a          903 c          922 g          434 t
ORIGIN      
        1 cgacgcgacg ctcgtcgcgc gcgtcgagcc atcgcttcgc cccgccccgg ggcaatcgct
       61 gcacgcgtgg ctcgatcccg cgcggctgca cctgttcgtg cctgagagcg gagtggggct
      121 gtggcacgcg ccgtaacggc cgcgacatgg tcgcgcggcc tccgcatgct cgcgctgccg
      181 ttgatcgtcg cggcggtggt gctggtcgca atcccggcgg cgatgacgat cctgttcgcg
      241 ttcaccgact acgacgcggt gtcggcgccg cgctggaacg gcctggacaa cttcgcccgg
      301 atgtggcggg atccgatctt tgcgaccagc ctgtggaact cgctgatctt cgtggtgatc
      361 gcggtgccgc tgcgcctgtt cgcggccgtc ggcacggcgc tgctcctgtc gggccgccgt
      421 cgcggaaccg cgaccgggcg ggcactggtg tacctgccga ccgtggtgcc cgacatcgcc
      481 tacgcgctgc tgtggctgtg ggtgttcaac ccggtctacg ggccgctgtc gatggtgctg
      541 cgagcggtcg ggttgcccgg tcccgaattg ctggtctcgg cctggggtgc gcggttcgcg
      601 gtcgcggcga tgacggcctt ccagatcggt gagggcttca tcgtcgccct ggccgttcgt
      661 aacgacatcc cccacgagct ctacgagctc gccgagatcg acggcgcccg gccgtcctgg
      721 acgcttcggc gcgtgaccct gccgctgatg atgcccgcgc tgctgctgct ggcggcccgc
      781 gacgtggtct tgtccttcca agcgaacttc gtcccggctt tggtgctcac cggcggcggc
      841 ccggagtacg ccacgacctt cctgccgctg tacacgtatc aaaacgcctt cgagtttctc
      901 cggttcgggt acgcttctgc catgacgttg accatgttcg tggtgacggc ggcgatgatc
      961 ggcatccagt tcctggtcgt tcgccgccgg cgggaagggt ggagcgcatg acctcgtggg
     1021 aacgcttcaa ggcggtcctg aacttcatcg gccgtagcag caaacgcatc gcggtcacgg
     1081 ccgtgggtct gacggtgctg gcggccggca tcgcgatgct cgcgcttccc ggtcccggcc
     1141 ttctggtcat catcctgggt ctggcgatcc tggccagcga gtacgtgtgg gcgcggcgcg
     1201 cgctcgacat ggcgaagcgc aaggccaacc aggccaagaa gaaggcgggc ggcatgttcc
     1261 gccgcaacaa caagaagtcg tcggtctagt cggtcttcag cgggctgggc tgcgttccgg
     1321 ggttggtgtt gaatcctggt gtcggcgcgt ccttgatcgc gggatcgttg agcaccatca
     1381 acacccacgt ccggctttgg tcgcacatga tcgagcgcgc gcggaagatg ccgtagcgct
     1441 cgcccttcaa gaagttgtcc tcggcctcgc ccggctcgga ctcgccgact ccgagcgacc
     1501 atccgtgctt cggccacacc ccgagggcgt gccgcacgaa gtcggtcaag tcccccgata
     1561 ccgagaagac cacgcgccgc aggccgcccg cggccggcgg cacgtcgatc gcgtacgagc
     1621 cctccggcat cggcaggtcg tcgggatacc atccaggccg ggcgatctcg ggctgcggct
     1681 gcaagcagtt gcccgtcagc gtcgcgggtg tggacggcac cgcggccggc cggccgtcgc
     1741 cgccgcgtga cgcagcgaat gcgacggcgc ccccgatcac gagcagaagg gcgatagcgg
     1801 cgaaacgcgc gcgggagctc atcgagcggt catatttgcc gccgcacggc gcgcgcgcaa
     1861 ggcaaccgcg ttaatttgtg gatttcgtcc gcttcgcgaa cgctccgaac cgccgcgggc
     1921 cggaggaacc cgccgagggc gccccgaatt cttaaggaga tccttcaggc aggtcaagct
     1981 cgagtaaaac cggaggggac tgttccatga gggaacgaag cgaacgcagg ccggtcatcg
     2041 cactcgccgc ggccgcggcg gtgctcgccg tcgcgggcat catgcccgcg ggcgcgcagg
     2101 agccgctcac ggccggcgga ttcgccggcg cggcggacgg gtcctttccc gcgcgtctgg
     2161 cggccgagca ccccatggcg gccgagtatc cgctgacctg ggcccacccc agcggcgagc
     2221 cgatcaccgt cacgcagccc gacggcaggc gcatccaggt catcggaacc gacatgaacg
     2281 tcggcggcgt cagcgagacc gtggacggct actcggtgat gcgcgacccc gcgagcggat
     2341 ggtgggtcta tgcgctcaag ggcgagaacg gctgggaggt tccgaccgag cgccgggtcg
     2401 gcatcgactc gtccggcggc attcccaagc acgccgcgcg aacgaagtcg gtctggatcg
     2461 acggacgcgg caaggacgct cgcgagcaga tcttcgaggc gctgcggcag aacacgatcc
     2521 agctgcaggc cacggccgcc gcggcggccg cgggcggcgc caccgtcttc aagatcccgg
     2581 cgctgatgct cgagacccac ggcccgttcg cggacgagtc cacccctgac ttcttc
//
LOCUS       DATLFO010000287         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_16328, whole genome shotgun sequence.
ACCESSION   DATLFO010000287 DATLFO010000000
VERSION     DATLFO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16328"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="nusA"
                     /locus_tag="VM841_11645"
     CDS             complement(<1..843)
                     /gene="nusA"
                     /locus_tag="VM841_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HVL90874.1"
                     /translation="MNYEFIEALRNLEREKGIPFESLLQGLEQALAAAYKRSMPEDKG
                     SRVEVHPENGEITVFQHDLDEEGSPIRDFDGNFANEVQITPGNFGRIEAQTAKQVILQ
                     KIREAERDMTFGEYAGRAGDIVSGIVQQSDSRFTLVDLGRGIEALLPQAEQIPGESYD
                     HGDRVKAYIVEVRRSAKGPSIYLSRTHPGLVKRLFELEVPEIADNIVEIKAIAREPGH
                     RTKLAVMTNDPAVDPVGACVGPKGSRVRNVVGELRGEKLDIIPWDDDPATFVGNALSP
                     AKVKE"
     gene            complement(878..1318)
                     /gene="rimP"
                     /locus_tag="VM841_11650"
     CDS             complement(878..1318)
                     /gene="rimP"
                     /locus_tag="VM841_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HVL90875.1"
                     /translation="MADSEALLTERVRSLAEPVLGRHDAELVELAVKRGPIYLVRLTV
                     DREDGIDLETCAKVSEELSRLMDADDPIPGRYTLEVTSPGADRPLRTARDFRRNEGRT
                     VRVVMASGNQVEGPIESIEEERVKVGGDWVALTDVAKAKIVLPW"
     gene            complement(1463..2893)
                     /gene="proS"
                     /locus_tag="VM841_11655"
     CDS             complement(1463..2893)
                     /gene="proS"
                     /locus_tag="VM841_11655"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HVL90876.1"
                     /translation="MALPKQSDDFPAWYQEVVKGADLAENSMVRGTMVIKPYGFAIWE
                     AIQRAFDDRIKATGHENLYFPLFIPYKLLEREAEHVEGFAPEVAVITHAGGEKLEEPF
                     VVRPTSETIIWETYARWVQSYRDLPLLYNQWCNVVRWEKRPRLFLRTTEFLWQEGHTA
                     HESAEDAIAEQRRILHEVYVDAARTVLGLPVVPGHKSASERFAGAVDTMTIEALMRDR
                     KALQAGTSHYLGQNFARAYGVQFQNRAGELEYAYATSWGVSTRLVGGVIMTHGDDKGL
                     RLPPRIAPVQVVIVPIYRADDERGRVLEAANAIKAELIAAGVRVKVDDRDEYRPGYKF
                     NEWELKGVPVRLEIGPRDLDGRQVTLARRDSGEKGPRPLDGLAASIPSVLDEIQQTLY
                     DQAEAFRDEHTFRPADYAEMGSLLADPGGFMTAGWCGEESCEQKVKGDYRATIRYLPL
                     EPEPATGSCLVCGNPAAHEATWAQAY"
BASE COUNT          472 a         1095 c         1088 g          570 t
ORIGIN      
        1 ctctttgacc ttggcgggcg agagcgcgtt gccgacgaac gtcgcgggat cgtcgtccca
       61 cgggatgatg tcgagctttt cgccgcgcag ctcgccgacg acgttgcgca cgcgggagcc
      121 cttggggccg acgcaggcac cgaccggatc cacggccggg tcgttggtca tcacggccag
      181 cttggtgcgg tgaccgggct cgcgggcgat cgccttgatt tcgacgatgt tgtccgcgat
      241 ctcgggaacc tcgagctcga acagccgctt gaccaggccg gggtgggtcc ggctcaagta
      301 gatgctcggg cccttcgcgc tgcggcgaac ctccacgata taggccttga cacggtcgcc
      361 gtggtcgtag gattcgcccg ggatctgctc ggcctgcggc agcagcgcct cgatgcctcg
      421 cccgaggtcc accagcgtga agcgcgagtc cgactgctgg acgatgccgg acacgatgtc
      481 gccggccctc ccggcgtatt cgccgaaggt catgtcccgt tcggcctcgc ggatcttctg
      541 caggatcacc tgcttggcgg tctgggcctc aatgcggccg aagttgcccg gcgtgatctg
      601 cacctcgttg gcgaagttgc cgtcgaagtc ccggatcggg ctgccttcct cgtccagatc
      661 gtgctggaag accgtgatct ccccgttctc gggatggacc tcgacgcggg agcccttgtc
      721 ttcgggcatg gagcgcttat aggcggcggc gagcgcctgc tcgagcccct gcagcaggga
      781 ctcgaacggg atgcccttct cccgttcgag gtttcgcagc gcttcgatga actcgtagtt
      841 catgtccgtt ccgtctccgc tctccgtgcg gccgcgttca ccacggcagc acgatcttgg
      901 ccttcgcaac gtccgtcagc gcgacccagt cgccgccgac cttcactcgt tcctcttcga
      961 tcgactcgat cgggccctcg acctggttac cggaggccat gaccacccgc accgttcgcc
     1021 cttcgttccg ccggaaatcg cgagccgtcc gaagcggccg gtccgcgccc ggcgacgtca
     1081 cctcgagcgt gtagcggccg gggatcggat cgtcggcgtc catcagacgc gacagctcct
     1141 cgctgacctt ggcgcaggtt tcgaggtcga tgccatcctc gcgatccacc gtcagccgga
     1201 ccaggtagat cggtccccgc ttgacggcga gctcgacgag ctccgcgtcg tggcggccga
     1261 gcaccggctc ggccagggac cggacgcgct ctgtcagcag cgcttccgaa tcggccatat
     1321 gaaacctccg gcaaacaaaa agtgggccga tgcccactcc gcgacgacgc ttcgtcttgg
     1381 gacaattata cacgcggccc cgtggcggct cttgctcaag ggaaagccgt gagccggaca
     1441 aacggcaacg attcgggcgt ggtcagtagg cttgggccca ggtggcctcg tgtgccgcag
     1501 ggttgccgca caccaggcag gagcccgtgg ccggctcggg ctcgagcggc aggtagcgga
     1561 tggtcgccct gtagtcgccc ttcaccttct gctcgcagga ctcctcgccg caccatcccg
     1621 cggtcatgaa cccaccggga tcggcgagca gggaacccat ctcggcgtag tccgcgggac
     1681 ggaaggtgtg ctcgtcgcgg aaggcctcgg cctggtcgta gagcgtctgc tggatctcgt
     1741 cgagcacaga ggggatcgac gccgcgagcc cgtccagcgg ccgcgggcct ttctcgccgc
     1801 tgtcccgacg cgccaaggtc acctgtctcc cgtcgaggtc gcgcgggccg atctcgagcc
     1861 ggacgggcac gcccttgagc tcccattcgt tgaacttgta gcccggccgg tactcgtcgc
     1921 gatcgtccac cttgacgcgc acgcccgcgg cgatcagctc ggccttgatc gcgttcgcgg
     1981 cctcgagcac ccggccccgt tcgtcgtcgg ctcgataaat ggggacgatg acgacctgaa
     2041 ccggggcgat cctgggcgga aggcgcaggc ccttgtcgtc gccgtgggtc atgatcacgc
     2101 cgccgaccag gcgggtggat acgccccacg aggtggcgta ggcgtattcg agctcgccgg
     2161 cacggttctg gaactgcacc ccgtaggccc tggcgaagtt ctgaccgaga tagtggctgg
     2221 ttcccgcctg cagtgccttt cggtcgcgca tcagcgcctc gatcgtcatg gtgtcgacgg
     2281 cgccggcgaa gcgttccgat gcggacttgt gcccgggcac gacgggaagg ccgagcacgg
     2341 tgcgggccgc gtccacgtac acctcgtgca ggatgcgccg ctgctcggcg atcgcgtcct
     2401 cggcggactc atgcgcggtg tgcccttcct gccacaggaa ctcggtggtg cgcaggaaca
     2461 ggcgcggccg cttctcccag cggaccacgt tgcaccactg gttgtagagc agcggcaggt
     2521 cgcgatagct ctgcacccag cgggcgtagg tctcccagat gatggtttcc gaggttgggc
     2581 gaacgacgaa cggctcttcc agcttctcgc cgcccgcgtg cgtgatcacc gcgacttcgg
     2641 gcgcgaatcc ctccacgtgc tcggcttcgc gctcgagcag cttgtagggg atgaacagcg
     2701 gaaagtagag gttctcatgg ccggtcgcct tgatccggtc gtcgaaggcg cgttggatgg
     2761 cctcccagat ggcgaagcca tacggtttga tgaccatcgt gccgcgaacc atcgagttct
     2821 cggcgaggtc ggcacccttc acgacctcct ggtaccaggc cggaaaatcg tctgactgct
     2881 tcggaagggc catgggcgcg aaggatacca gcccgaggtg gtcgccctta ccgcccccgc
     2941 ggggccggct gcccatgcgg gaccctgtgc aaccaagttg cagccgagcc gggcctgatg
     3001 ccccatgcgg gaccctgtgc aaccaagttg cagcgggggc gggccgggtg cccccgggac
     3061 ccccgcgtca ccattttgcc gggccgctcg aagacggcgg ccggctcggg cgcgggaagg
     3121 ctgagcgacc cccgtgacgg cgacgccggc ccatggtcgc tgggcacggg gccgcttcgc
     3181 gcggcagctc gtgggcgcgt gcaacttggt tgcacagggt gtcgg
//
LOCUS       DATLFO010000288         3643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_135958, whole genome shotgun sequence.
ACCESSION   DATLFO010000288 DATLFO010000000
VERSION     DATLFO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135958"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VM841_11660"
     CDS             complement(<1..369)
                     /locus_tag="VM841_11660"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HVL90877.1"
                     /translation="MLPRSGRYTKAAVLHDHLWRTPIDGVSFGDADGIFRRCMRELGV
                     PFLRRWLMWAAVRLNSLWKSRFGDRLGDLPHVLLVALFPGLFVLAGGLIVVVLLAGFY
                     LLELASSAVLALLRLIPGVRR"
     gene            complement(281..1084)
                     /locus_tag="VM841_11665"
     CDS             complement(281..1084)
                     /locus_tag="VM841_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4344 domain-containing metallopeptidase"
                     /protein_id="HVL90878.1"
                     /translation="MNMTFVSRSAAIVLAAVLALTACGSDAPEPSARGSQTPAETVED
                     KGDFTVAFATPKDSAFADYPDLLREFAEESAGAVNEILALPQDIPLTFDEIGEENAYY
                     DPEEEAITFGYEYVAALVDLAPKLRETEDEQDQFIYANVENVMLHELAHALVHILELP
                     VLGKEEDAADGLATLIQIEAFEEGTAYAIDVADGWGAVAENPGSLDDSAFADEHSLNA
                     QRFYATVCWVAGSSPKAMAQVASAVWPLHQGRGAARSSVAHADRRRQLR"
     gene            complement(1130..1747)
                     /locus_tag="VM841_11670"
     CDS             complement(1130..1747)
                     /locus_tag="VM841_11670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HVL90879.1"
                     /translation="MTIHPVAGHQRVVFLKPLITSPLIDVGDYTYYDDPDDAAAFEQR
                     NVLYHYGPERLTIGKYNALASGVRFIMNGGNHATDGPSTFPFPIFGGDWAAAGPPRGA
                     PSRGDTVVGSDVWIGNGATIMPGVTIGHGAIIATGAVVTRDVPPYAIAAGNPARIVRH
                     RFSEAEVATLLDIAWWDWPAQKVARHLDAIMRGTVEQLALAHTRP"
     gene            1912..2697
                     /locus_tag="VM841_11675"
     CDS             1912..2697
                     /locus_tag="VM841_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL90880.1"
                     /translation="MSEKVTTEDRGPVRILRINRPEVRNCVDGETAVGLGRAIEAFAA
                     DDAARVLVVTGEGDLSFCAGADLKNAQTLLGHEYVDKAAPMGFARLDPGKPTIAAING
                     YCFAGGFELAAWCDIRVAAANAEFGCLSRRWGVPYTDGGTQRFSRIIGMGNALYMLTT
                     GARIDVRRAYEMGFVQEIVARGEALNRAIALAEQVAAYPNQPGIRADRAAALQGFTMD
                     LDDALLLEARLVRPTLFGDEMAAGLERFAAGARDESPRPPSVG"
     gene            2775..3128
                     /locus_tag="VM841_11680"
     CDS             2775..3128
                     /locus_tag="VM841_11680"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HVL90881.1"
                     /translation="MSECLFIGRFQIKDGKQEDFGQVAKRIMAAVAKEPGVKRYDWYE
                     GKDGAVMAVEIYVSGEAVMEHMTGEAGALLPEIFEFADVVGIDLYGEPGATVREAMAA
                     FPITYYPEPTFSPGG"
     gene            3158..>3643
                     /locus_tag="VM841_11685"
     CDS             3158..>3643
                     /locus_tag="VM841_11685"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HVL90882.1"
                     /translation="MDTPRPRRRGRVVVPDGTGHTEDLELLERHGPQRGEESWRALRI
                     LSEFVDGFDALSAIPPAISVFGSARVREGDPAYDGARVVGRAIADAGFAVITGGGPGV
                     MEAANRGCQEAGGLSIGCNIELPFEQGMNAYVDLGIDFRYFFVRKTMFVKYAEGFVVF
                     PG"
BASE COUNT          618 a         1158 c         1257 g          610 t
ORIGIN      
        1 tctgcgcacg cccgggatca gccgcagcag cgcgagcact gccgacgatg ccagctccaa
       61 cagatagaaa ccggcgagca gcacgacgac gatcaggccg cccgccagga cgaacagccc
      121 cgggaacagc gcgacgagca ggacgtgtgg gagatcgccg agccggtcgc cgaaccgtga
      181 cttccaaagc gagttcagcc ggaccgcggc ccacatgagc cagcgacgca ggaacgggac
      241 gccgagctcg cgcatgcagc gccggaagat tccgtcggca tcaccgaagc tgacgccgtc
      301 gatcggcgtg cgccacagat gatcgtgcag caccgcggcc ttggtgtagc ggccagaccg
      361 cggaagcaac ctgtgccatc gccttcggac tgctgcccgc tacccagcac accgtcgcgt
      421 agaagcgctg cgcgttgagg ctgtgctcgt cggcgaacgc cgaatcgtcg agcgagccgg
      481 gattctcggc gacggcgccc cagccgtccg cgacgtcgat cgcgtaggcg gtgccctctt
      541 cgaaggcttc gatttggatc agcgtcgcca gcccatcggc agcgtcctct tccttgccga
      601 gcaccggcag ctcgaggata tgcaccaacg catgcgccag ctcgtgcagc atcacgttct
      661 cgacgttggc gtagatgaac tggtcttgct cgtcttcggt ctcgcgcagt ttcggcgcca
      721 ggtccacgag cgcggcgacg tactcgtagc cgaaggtgat cgcttcctct tccggatcgt
      781 agtaggcgtt ctcttcgccg atctcgtcga acgtcagtgg aatgtcctgt ggcagcgcca
      841 gaatctcgtt caccgcgccg gccgattctt cggcgaactc acgaagaagg tccggataat
      901 cggcgaaggc agagtccttc ggcgtcgcga aggccacggt gaagtcaccc ttgtcttcga
      961 cggtttcggc cggtgtttgg gatccccgcg cggacggttc cggcgcgtcc gacccgcacg
     1021 cggtcaaagc cagcacggcc gccaggacga ttgccgccga gcgagagaca aaggtcatgt
     1081 tcatgcgcgg cagggtagca gcgggcaccg gatgccgtgc gaagcggggt cagggccgtg
     1141 tgtgcgccag ggcaagctgt tcgaccgtgc cgcgcatgat cgcatccagg tgccgggcca
     1201 ctttctgtgc gggccaatcc caccacgcga tgtccaacag cgttgcgacc tccgcttccg
     1261 agaagcggtg acgaacgatg cgcgccggat tgcccgcggc gatcgcatac ggcggaacgt
     1321 ctcgcgtgac gacggcgccg gtcgcgatga tcgctccgtg cccgatggtc acgcccggca
     1381 tgatggttgc gccgtttccg atccacacgt cgctgcccac gacggtgtcg cctcgtgacg
     1441 gcgcgccccg cggcggaccc gctgcggccc agtcgccgcc gaagatcggg aacggaaacg
     1501 tggacgggcc gtcggtggcg tggttgccgc cgttcatgat gaagcgcacg ccgctggcca
     1561 gcgcgttgta cttgccgatg gtcaagcgtt cgggcccgta gtggtagagc acgttccgct
     1621 gctcgaacgc ggcagcgtcg tcaggatcgt cgtagtacgt gtagtcaccg acgtcgatca
     1681 acggcgaggt gatgagcggc ttgagaaaca cgacgcgctg gtggccggcg acgggatgga
     1741 tggtcatggg atcgggtatc acgggaacgc tcctgttcgc ggagatggcg gcacgaggac
     1801 gcgttcagat caccatgatg tctccagtgt gcgtctcatt gccggcgcgc gcaaccaatt
     1861 ttcgactcgc gcggctagca tgagcgccgg cacgcgaacg aggaggccgt ggtgagcgag
     1921 aaggtgacga cggaagaccg ggggccggtt cgtatcctgc gaatcaaccg gcccgaggtt
     1981 cgcaactgtg tggacggcga aaccgcagtg ggtctcggcc gtgcgatcga agcgtttgcg
     2041 gccgacgacg cggcacgggt gctggtcgtg accggtgagg gagatctgtc gttctgtgcg
     2101 ggcgccgacc tgaagaacgc acagacgctg ctcgggcacg agtatgtcga caaggccgcg
     2161 ccgatgggct tcgcgcggct ggatcccggc aagcccacga tcgccgcgat caatggttac
     2221 tgcttcgccg gcgggttcga gctggcggcc tggtgcgata tccgtgtcgc ggccgcgaac
     2281 gccgagttcg gatgcctgtc gcgccggtgg ggtgtgccct acaccgacgg aggaacgcag
     2341 cgtttctcgc gcattatagg catgggcaac gcgctgtaca tgctgacgac gggcgcgcga
     2401 atcgacgtgc ggcgcgccta cgagatgggt ttcgttcaag agatcgtggc ccgaggtgag
     2461 gcgctgaacc gcgccatcgc gcttgcggag caggtcgccg cctacccgaa tcagccgggg
     2521 attcgagccg accgagcggc cgcgcttcaa ggattcacca tggatctgga cgatgcgctg
     2581 ctgctcgaag ctcgtcttgt ccggccgact ctgttcggcg acgagatggc cgcgggcctc
     2641 gaacgtttcg cggccggtgc tcgggatgaa tcgccgcggc caccgtcggt ggggtgaatc
     2701 ggccgaccct tgcgttcccg ggaagcgctt gcttatcgtg atcggaccgg ttcaccgaaa
     2761 aggggggctt tcccgtgagc gagtgcctgt tcatcggccg tttccagatc aaggacggca
     2821 agcaagaaga cttcggccaa gttgcgaagc gaatcatggc cgccgtcgcc aaagagcccg
     2881 gcgtcaagcg ctacgactgg tacgaaggca aagatggtgc cgtgatggca gtcgagatct
     2941 acgtctccgg cgaggccgtc atggagcaca tgaccggcga agcaggagcg ctgcttcccg
     3001 agatcttcga gttcgccgat gtcgtgggaa tcgacctcta cggcgagccg ggtgcgaccg
     3061 ttcgtgaagc gatggccgcg ttcccgatca cgtactaccc cgagccaacc ttctcgccgg
     3121 ggggctaaga aacgctgccc tgcgataggg tcggggcatg gacacgccac gaccccgtcg
     3181 ccgcggccgc gtcgtcgtgc ccgacggcac cggccacacc gaggatctcg agcttctcga
     3241 acgtcacggc ccgcagcgcg gcgaggagtc ctggcgcgcg ctgcgcatcc tcagtgagtt
     3301 cgtggacggc ttcgacgcgc tgtcggcgat cccgcccgcg atcagcgtgt tcggatccgc
     3361 gcgggtgcga gagggtgacc ccgcctacga cggcgcgcgc gtcgtcggcc gtgccatcgc
     3421 cgacgcgggc ttcgccgtca tcaccggcgg cggccccggc gtgatggagg ccgcgaaccg
     3481 cgggtgccag gaagcgggcg ggctctcgat cggatgcaac atcgagcttc cgttcgagca
     3541 gggcatgaat gcctacgtgg acctcggcat cgactttcgg tacttcttcg tgcgcaagac
     3601 catgttcgtg aagtacgcgg aaggcttcgt cgtcttcccg ggc
//
LOCUS       DATLFO010000289         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_37555, whole genome shotgun sequence.
ACCESSION   DATLFO010000289 DATLFO010000000
VERSION     DATLFO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37555"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VM841_11690"
     CDS             <1..1254
                     /locus_tag="VM841_11690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HVL90883.1"
                     /translation="TPTTNGVAEGAGLTIVSIVGRRVGVIARNHFPDDGLEDLVRESE
                     AACEGKPEAEDYMPLLEGTAPAGDWSAPAATTDITVFDRFTPQLAGMFDRAGAADVNT
                     YGYAEHTASTVWLATSSGVRARDHTPSGRVEVTAKSTDFARSAWAGVGTADFTDVRLG
                     TLFDGLAQRLQWSERKVEVPAGKYEVVLQPAAVADMLIYMYWSSSARDADEGRTVFHK
                     TGGGNRLGEKMFAGGVNVYSDPAEPGLTGERFVRTAGSSSYSSVFDNGLPTRRVDWVH
                     DGALNALVTTRHWAARSDGEPAPLISNLVFPAEGRSLEEMVAETERGLLVTCFWYIRA
                     VDPQTLLLTGLTRDGVFLVEGGEVVGAANNFRYNMSPVAMLAQTTEIGRSAPTLPREF
                     EEFGLTKMPPIRVADFNMSSISEAT"
     gene            1295..1834
                     /locus_tag="VM841_11695"
     CDS             1295..1834
                     /locus_tag="VM841_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL90884.1"
                     /translation="MPKIAIVGSFSTGKTTLAEIVAERLGLSLLPEVAREVAAMGFKL
                     DKDATPEVETLIFLKQLRNEMTHEHFVGDRSLIDVMAYAGWVLDNQPPRPESALWEAC
                     VNIAEHQLRSQYSHVFYLPIEFPIVPDGLRPLDPDFQSEIDARMRALLDRHAISFETL
                     TGSVEDRVDALVDRVRTSV"
     gene            1836..>2956
                     /locus_tag="VM841_11700"
     CDS             1836..>2956
                     /locus_tag="VM841_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HVL90885.1"
                     /translation="MAPGAEFADVFERLRAALSDLRARHTSGAGGLAVARARAAAMDE
                     ALGQLWARVAPPAGSLALVAIGGYGRGELSPASDLDLMVLHAGEPSAREVSERLYYAL
                     WDNAGVPVGHATRTVKECLRLAKTNLEAETSFLTPRLLAGAEASAAEFTSATLAATRK
                     RRSGFVRDVREMMRARHVVSGSATSQVEPNLKEGIGGLRDLHVMGWFERVEGANLLSS
                     ADHAHLEEAADLLLRVRDDLHDLTGSASDVLLLQHQRPIALRLGYSDAARPAEDELMR
                     GLFTATRGVELIVSSVVADLVAGPPRRIRSAAGGCFAEQDGRVVVARTPDLAAEPERA
                     LEAFALGVPPGSGALHALEDALAQVGTLPWTDAVRRAFLG"
BASE COUNT          432 a          993 c         1069 g          462 t
ORIGIN      
        1 acgccgacaa cgaacggggt cgccgaaggg gcgggcctga cgatcgtgtc gatcgtcggc
       61 aggcgcgtcg gcgtgatcgc gcgcaatcat ttccccgacg acggactgga ggacctcgtg
      121 cgggagtccg aggcggcctg cgaggggaag ccggaggccg aagactacat gccgctactg
      181 gagggcacgg ctcccgccgg cgactggagc gctcccgcag cgacgacgga cattactgtg
      241 ttcgaccggt tcacgccgca actggcgggg atgttcgacc gcgcaggcgc cgcggacgtg
      301 aatacgtacg gctacgcgga gcacaccgcg tccacggtgt ggctggcgac gtcgtcgggt
      361 gtgcgtgcgc gcgaccacac gcccagcgga cgggtggagg tgaccgcgaa gtccaccgac
      421 ttcgcacgct cggcctgggc cggcgtcggc accgccgatt tcaccgacgt acgcttgggc
      481 acgctgttcg acggactcgc ccagcggctg cagtggtcgg agcgcaaggt cgaggttccg
      541 gcggggaagt acgaggtggt tctgcagcca gcggcggtcg ccgacatgtt gatctacatg
      601 tactggagtt cgtccgcgcg tgacgcggac gagggtcgca ccgtgttcca caagaccggc
      661 ggcggcaacc ggctcggcga gaagatgttc gccggcggcg tcaacgtgta ttccgatccc
      721 gccgagcccg gcctcaccgg agagcgcttc gtgcgcaccg cgggctcgag cagctactcg
      781 tcggtgttcg acaacggtct gccgacgcgc cgcgtcgact gggtgcacga cggggcgctg
      841 aacgcgctcg tcacgacgcg acactgggcg gctcgttcgg acggcgagcc tgcgcctctg
      901 atcagcaacc tggtgttccc ggccgagggg cgctcgctgg aggagatggt cgcggagacc
      961 gagcgcgggc tgctggtgac atgtttctgg tacatccgcg cggtcgatcc ccagacgctt
     1021 ctgttgaccg gtttgacgcg cgacggcgtg ttcctcgtcg agggcggcga ggtggtgggg
     1081 gcggcgaaca acttccgcta caacatgagc cccgtggcga tgctcgctca gacgacggag
     1141 atcggccgct cggccccgac attgccgcgc gagttcgagg agttcgggct caccaagatg
     1201 ccgccgatcc gcgtcgccga tttcaacatg agctccatca gcgaggcgac gtaaggccgc
     1261 ggccgtcgcg ctcgcgtgag ataggctgca gcgtatgccg aagatcgcca tcgtcgggtc
     1321 gttctcgacc gggaagacca cgctcgccga gatcgtcgcc gagcggctcg gcctctcgtt
     1381 gttgccggag gtcgcgcgcg aggttgccgc catgggcttc aagctcgaca aggacgcgac
     1441 gccggaggtc gagaccctga tcttcctgaa gcagctgcgc aacgagatga cgcatgagca
     1501 cttcgtcggc gaccggtcgt tgatcgacgt gatggcgtac gcgggctggg tgctggacaa
     1561 ccagccgccg cggcccgagt cggcgctctg ggaggcatgt gtcaacatcg ccgagcacca
     1621 gctgcgtagc cagtactcgc acgtgttcta ccttccgatc gagttcccga tcgtccccga
     1681 cggcctgcga ccgctcgatc cggacttcca gagcgagatc gacgcgcgga tgcgcgcgct
     1741 gctcgatcgt cacgcgatca gcttcgagac gctgaccggt tcggtcgagg atcgtgtcga
     1801 cgccctggtc gatcgggtgc gaacctcggt ctgacgtggc tcccggcgcc gagttcgcgg
     1861 acgtcttcga acggctccgc gcggccctga gcgatctgcg cgcgcgccac acctccggtg
     1921 cgggtgggct cgcggtcgca cgcgcgcgcg ccgccgcgat ggacgaggcg ctcggccagc
     1981 tgtgggcgcg cgtcgcgccg ccggccggtt cgctcgcgct ggtggcgatc ggcgggtacg
     2041 ggcgcggcga gctctctcct gcttccgacc ttgacctgat ggtgctgcac gccggcgagc
     2101 cgtccgcgcg tgaggtatcc gagcgcctct actacgcgct gtgggacaac gccggcgttc
     2161 ccgtcggcca cgccacgcga accgtgaagg agtgcctgcg cctggccaag accaacctcg
     2221 aggcggaaac ctcgttcctc acgccgcgcc tgcttgccgg ggcggaggcg tcggcggcgg
     2281 aattcacctc cgccacgctg gcggctacgc gcaagcgccg cagcgggttc gtacgtgatg
     2341 tgcgcgagat gatgcgcgcg cgccacgtgg tgagcggatc ggccacgtcg caagtggagc
     2401 ccaacctgaa ggaaggcatc ggcggtcttc gcgaccttca cgtcatgggg tggttcgagc
     2461 gcgtcgaggg agcgaacctg ctgagcagcg ccgaccacgc gcacctcgaa gaggcagccg
     2521 atctgttgct gcgcgtgcgc gacgatctgc acgacctcac cggatcggcc tcggatgtgc
     2581 tgctgctgca gcatcaacgg ccgatcgcgc tgcgcctcgg ctattccgac gcggcgcgcc
     2641 ctgccgaaga cgagttgatg cgcgggctgt tcaccgcgac gcgcggcgtc gagctcatcg
     2701 tgtcctccgt cgtcgccgac ctggtggccg gaccgccgcg ccgcatccgg tccgcggcag
     2761 ggggatgctt cgccgagcaa gacgggcggg tggtggtcgc gcgcacgccg gacctcgccg
     2821 cggaacccga acgcgcgctc gaggcgttcg cgctcggcgt accgcccggc agcggcgcgc
     2881 tgcacgcgct cgaggacgcg ctggcccagg tcggcacgct gccgtggacc gacgccgtgc
     2941 gccgcgcgtt cctcgg
//
LOCUS       DATLFO010000290        12398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_125262, whole genome shotgun sequence.
ACCESSION   DATLFO010000290 DATLFO010000000
VERSION     DATLFO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125262"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VM841_11705"
     CDS             <1..735
                     /locus_tag="VM841_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVL90886.1"
                     /translation="MVLHVSDTHFRSTLKAMPRFFERKLARLTPDLVVGTGDFLGDTR
                     SAQQCAEMLSAVPARLGRVFVLGSNDYYAPVPKNPLRYFGPTRRPYRHGAKNETWTDL
                     VSALEARGWQFLRNDRTRLDGIEISGLDDPHIGRADLTAAPPRNAVGFRLAVVHSPQV
                     ARKLAENGFDLILSGHTHGGQIRVPGYGALVTNTPGLPRRMARGAHRLGGAWLHVTAG
                     LGTSKFTPIRFACRPEACLLELVPRA"
     gene            867..1268
                     /locus_tag="VM841_11710"
     CDS             867..1268
                     /locus_tag="VM841_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVL90887.1"
                     /translation="MSSNAKIDLLKTVPLFAECSKHELEQIARLVDQVTFPAGKVAIH
                     EGEPLGLECIVVIDGRLEVSRGGEVIAELGPGDIAGEMSLLELVPRSASVTAATDVEA
                     LVMDPRSFNSLMDDVPRVAEAIAEVVRKRHA"
     gene            complement(1265..2293)
                     /locus_tag="VM841_11715"
     CDS             complement(1265..2293)
                     /locus_tag="VM841_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL90888.1"
                     /translation="MRIAQICPPWFSVPPAGYGGIEWVVALLADGLAERGHDVTLFAS
                     GSSTTKAKLVSEFDEPPGGTMLGQIWFEAVHAVSAYKHKDEFDIIHDHCGLVGPSIGA
                     NIGGPPVVHTLHGPFTEQAKRYYRLTSPPLHLVAISEAQRAACPDLNYAGTVHNGIDL
                     SAYRMRADKEDYLLFLGRINREKGPEIAVEVAARAGMKLKMAVKMTEPFEQQYWREVI
                     EPRLTGSEEVLGEISVEEKATLLAGARATVFPIQWPEPFGLVMTESMACGTPVLAFPR
                     GAAPEVIVDGVTGYLPETIDEMVACVDRLDRIDPAACRAHVEARFGAASMVEGYERIF
                     KALLAGSR"
     gene            complement(2450..4945)
                     /gene="treY"
                     /locus_tag="VM841_11720"
     CDS             complement(2450..4945)
                     /gene="treY"
                     /locus_tag="VM841_11720"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HVL90889.1"
                     /translation="MSRDTPVATYRLQLGPDLTFARVRAILPYLERLGVSHLYLSPIT
                     EARPGSTHGYDVTDPGAIRKALGGEEGFRKLAAAARKHGLRILLDIVPNHVAASPDNP
                     WWEDLLVNGIDAESAFRFDLDLERAADAVVLPVLKHPLERELRARTIKVTTAGGAVRV
                     RYGDLALPTAPRTWTILFERNGSGLRRRPGEDRLRAFLDAQPWRLASWRGGDRKIVYR
                     RFFDITDLAGVRVEDPRVFDDVLGLAIRLVRDGLVDGLRLDHIDGLRDPAAALRRLRG
                     ALGSDAWILIEKILEADEPIRAGWPVDGTTGYEFLNALNGVFVDRAGFEALRTDWMRR
                     TGDRTPFEQIVHDGKRNALETLFPAEAETLAGLLRSLDDSIDEESYAGAVVAVTVHLP
                     VYRTYVASTHPETEDRRLIERAVRHARGEADAAALRVLRAALLLEHGGSMRVQRDLVA
                     RWQQLSGPAMAKGLEDTALYRHAPLASLNEVGGRADVATDLAFFSRFNAARLHTHPLS
                     LAATSTHDTKRGEDVRARLNVLSEIPEEWTRLLDRWERRHRRFVGNAGEHESPDAAER
                     ILLYQTIVGAWPLRGSRQRLAARVADYARKAAREAKRHSSWRKPDHKHERALRRWVQA
                     VVADDVFARDVDRLLRIVGLHGAIGSLAQVLLKVVAPAVPDFYQGTELWSLTLVDPDN
                     RGRVDFRRRARLLERAERAATFDPRDETTKMAVIARALRVRRDDPDLFARGRWVPLVA
                     DGLRAGQAVAVAREHEGRWAVAIAPRFLAAVTGARLPGRATWRDTAVTLPRGAPRRWR
                     DALSGRTAETPGGRLALDRTLAALPLALLRPSD"
     gene            complement(4935..7037)
                     /gene="glgX"
                     /locus_tag="VM841_11725"
     CDS             complement(4935..7037)
                     /gene="glgX"
                     /locus_tag="VM841_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HVL90890.1"
                     /translation="MRVWPGSPYPLGATWDGEGVNFAIFSEHAEGVELCLFDAPDDPK
                     ETLRVPLRERTDLIWHCYLPDARPGQLYGYRIHGPYAPAEGHRFNPNKLLLDPYGKAI
                     SGTVKWDDTLFGYRIGEDDLVPDERDSAAALPKCVVINPAFPWGDDRRPLVPWNRTFI
                     YETHIRGMTMLHPEIPDELRGTYLGMAQEPVINHLLSLGVTTVELMPIHQFVVERELY
                     ERGLTNYWGYNSIAFLAPDVRYATGGLGEQVNEFKTMVKTFHRAGIEVILDVVYNHTG
                     EGNHLGPTLSLRGVDNKSYYRLTPEDERYYMDFTGCGNSLNMLHPRTIQLIMDSLRYW
                     IADMHVDGFRFDLAPALARELYEVNRLGTFFDIIQQDPTISQVKLIAEPWDVGPGGYQ
                     VGNFPVGWAEWNGKYRDAIRKFWRGDPGLIPEVASRLSGSSDLYQASGRRTYASINFV
                     TCHDGFTLHDLVSYEQKHNEDNGEGNRDGADDNNSHNWGAEGPADDTRILRMRERIKR
                     NFLATLMLSQGVPMLSHGDEVGRTQRGNNNGYCQDNDKTWIHWDLDQDDRELLEFTRT
                     LTELFHRHPVLRRRSFFTGRTISDQGFKDLTWLRPDGNEMEQHDWDDGSNHVIGMLIH
                     GRATDEVDEQGRPASGETVLLLLNGGPRSRHFTLPKVGAGQWIEVLNTARPGERTVKT
                     DAVNLAAHSLIFLRFDEP"
     gene            complement(7041..8846)
                     /gene="treZ"
                     /locus_tag="VM841_11730"
     CDS             complement(7041..8846)
                     /gene="treZ"
                     /locus_tag="VM841_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HVL90891.1"
                     /translation="MNGDEMIGALPHDRGVRFRVWAPRAEQVELRITGADSVSMTRDG
                     DYFEALAADARPGTRYRYSLDDGEPRPDPASRSQPDGVHGDSEVVDPSAFAWTDDGWR
                     GLPLKDYVISETHIGTFTPEGTFRAAADRLKELTDLGVTAIEIMPVSQFPGERNWGYD
                     GVFSFAPQNTYGGPEGLRALVDACHAAGIAVIMDVVYNHFGPEGAHWREFGPYHTDEY
                     HGPWGDAINVDGSGSDEVRAFFIASARMWFFEYHMDALRLDAVHAIKDFSAGPFLREL
                     AMHKREWEAELGRPLQLIAETDQNDPKMVHALELGGLGMDAQWSDDFHHAVHALLTGE
                     RDGYYADFGSPQDLAKAYTDVFVYDGRRSVFRGRRHGAPASELPRERFVVCIQNHDQT
                     GNRMLGDRLAALVDFEALKLAAGALLYSPFVPMLFMGEEYGETNPFLYFTSHGETKLI
                     KAVRDGRKREFASFGWQGEPPDPQSAETFERSKLNRSLADEPRGRALRDWYRDLIALR
                     RRMPAMATGVPAAATARGPLVSVLRRAHGNEVLAVLNTSPEQASERLAGEWTVELDSA
                     DARWAGPGSPTNATVRSGETITRPGHSFLLLSRKD"
     gene            complement(8833..10746)
                     /gene="glgB"
                     /locus_tag="VM841_11735"
     CDS             complement(8833..10746)
                     /gene="glgB"
                     /locus_tag="VM841_11735"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HVL90892.1"
                     /translation="MAAREARETTSPLTDDDIYLFNEGTHYKLGEKLGSHMTQGGTHF
                     GVWAPNAERVSLIGDFNGWDDTAHPMSARGGSGIWETFVEGIGQGSIYKYRIHSRHTG
                     YRVDKADPFAVHHETPPKTGSIVWDDDYTWNDGEWMAGRGPRGGLASPMSTYEVHLGS
                     WRRAPEDGNRSLSYRELAGPLADYVEEMGFTHVELMPVTEHPFYGSWGYQASGYFAPT
                     ARYGTPQDFKHLVDTLHQRGIGVILDWVPSHFPSDEHGLGYFDGTHLFEHADPRQGFH
                     PDWESLIFNYGRNEVRAFLMSSAIHWLDRFHIDGLRVDAVASMLYLDYSRKDGEWIPN
                     EFGGRENLEAIAFLRRFNEDVYTAHPDVQTIAEESTAWPMVSRPTYVGGLGFGLKWDM
                     GWMHDTLEYFKKDPVHRRFHQHMLTFRSLYAWQENFVLPLSHDEVVHGKGSLLGKMPG
                     DEWQQFANLRALYGLMYATPGKKLLFMGAEFGQRGEWNHEASLDWHVLDYELHAGVQR
                     LVRELNRMYRSEPALHEMDAEAAGFEWVDASDAENSVIASIRRGRSTDDLILVAANLT
                     PVPRHNYILGVPRGGQWREILNTDAREFGGSGQGNAGGKEASPVGAHGRPRALTLTLP
                     PLSVLMLKSDERR"
     gene            complement(10753..>12398)
                     /gene="malQ"
                     /locus_tag="VM841_11740"
     CDS             complement(10753..>12398)
                     /gene="malQ"
                     /locus_tag="VM841_11740"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVL90893.1"
                     /translation="PLPRNLPFGYHRLRVEADAAAEARLIVAPPAMHDGPRAAGVFAP
                     VYAVRGERDYGCGDLGSFAGLIDVVSELGGSLVATLPILSAFMDEPFDPSPYAPVSRL
                     HWNEIYLDIDAVPEFADSEQARALAKELAPKIRGLRRKQDVDYRGVSAVKRRVLEACA
                     ASLSGKRRREMDAWLRENPVTLEYARFRAETERSGLPWQAWPGTAKLRRGGDAEKYHV
                     YAQWLMNEQLARAGREADDKGVHLYFDLPLGVHPDGFDTWRYGEVFAFGVSGGAPPDS
                     FFTKGQNWRFPPLHPERIRETGYSYVIESLRALLRHARILRIDHVMSLHRLFWVPDGF
                     DATDGVYVRSNPDEWYAILALESHRAGAAIVGEDLGTVPGEVRTAMARNNLLRSYVLQ
                     EELPDSPKQVPNPIPARALAGLNTHDMPTFASFWNGKDIDDRVGLGLFTPAQAEKERT
                     RRAKTTKRLSASLRGRKLLKGRGADAALDASLALLAGGRARMRCVSIEDLWLEERQQN
                     VPGTTSDEQPNWRHKLRYTLEEIRGDPAVAARIRTLTRR"
BASE COUNT         1937 a         4187 c         4261 g         2013 t
ORIGIN      
        1 atggtcctgc acgtctccga cacgcacttc cgatccacgc tcaaggcgat gccacgcttc
       61 ttcgagcgga agctggctcg cctgacgccc gacctggtgg tgggcaccgg agacttcctc
      121 ggcgacaccc gttccgcgca acagtgcgcc gagatgctct cggcggttcc cgcccggctc
      181 ggccgcgtgt tcgtgctcgg ctcgaacgac tactacgcac cggtgccgaa gaatccgctg
      241 cggtacttcg gtccgacgag gcggccgtac cgccacggtg ccaagaacga gacgtggacg
      301 gatctggtct cggcgctgga ggcgcgcggc tggcagttcc tgcgcaacga ccgaacccgc
      361 ctggacggca tcgagatcag cgggctcgac gatccccaca taggccgcgc ggacctgacc
      421 gcagcgccgc cgcgcaacgc cgtcggcttt cggctcgccg tggttcattc gccgcaggtc
      481 gctcgcaagc ttgccgagaa cggcttcgac ctgatcctgt cgggacacac gcacggcggt
      541 cagattcgcg ttcccggcta cggcgcgttg gtgacgaaca cgccggggct gccgcgccgc
      601 atggcccgcg gcgcgcatcg cctgggtggg gcgtggctgc acgtgacggc cggcctgggc
      661 acgagcaaat tcacgccgat ccgcttcgcg tgccgtccag aagcgtgcct gctcgaactc
      721 gtgccgcgcg cctagggcgc cggtcgagcg ccaccgggac cggcgatgcc cgaacctcaa
      781 gccggagcgg cccccacccc ccggccgcgc gatttagggg ataatggatc cttgtccact
      841 ccgtcctggc agggaggtcg tcgccaatgt cgagcaacgc caagatcgat ctgctgaaga
      901 ccgttcctct cttcgccgaa tgctccaagc acgagctgga gcagatcgcg cgtctcgtcg
      961 accaggtgac cttcccggcg ggcaaggtcg cgatccacga gggcgagccg ctcggcctcg
     1021 aatgcatcgt cgtcatcgac ggcaggctgg aggtttcccg gggcggggag gtcatcgccg
     1081 agctgggccc gggggatatc gcgggcgaga tgtcgctgct ggagctggtg ccgcgatcgg
     1141 cgtctgtgac ggcggcgacg gacgtggaag cgctcgtgat ggatccgcga agcttcaaca
     1201 gcttgatgga cgacgtgccg cgggtggccg aggcgatcgc cgaggtcgtg cggaagcggc
     1261 acgcctagcg cgagccggcg agcagcgcct tgaagattcg ctcgtagccc tcgaccatgc
     1321 ttgccgcgcc gaagcgcgcc tcgacgtgcg cgcggcacgc cgcaggatcg atgcgatcga
     1381 ggcggtccac acaggcaacc atctcgtcga tcgtctcggg caggtagccg gtcacaccgt
     1441 cgacgatcac ctcgggcgcg gcgcccctcg ggaacgcgag caccggcgtg ccgcaggcca
     1501 tcgactcggt catcaccagc ccgaacggct cgggccactg gatcgggaac accgtggcgc
     1561 gcgcaccggc cagcagcgtg gccttctctt cgacgctgat ctcgccgaga acctcttcgc
     1621 ttccggtcag gcgcggttcg atcacctcgc gccagtactg ctgctcgaac ggctcggtca
     1681 tcttgacggc catcttcagc ttcatcccgg cccgggcggc gacctcgacg gcgatctccg
     1741 gccccttctc gcgattgatg cgaccgagga acagcagata atcctccttg tccgcgcgca
     1801 tgcggtaggc ggacaggtcg ataccgttgt gaaccgtgcc tgcatagttg agatcggggc
     1861 aggcggcgcg ctgcgcctcg ctgatcgcaa ccagatgcaa cggaggcgac gtcagccggt
     1921 aatagcgctt cgcctgctcg gtgaacgggc cgtgcagcgt atgcacgacg ggcggtccgc
     1981 cgatgttggc cccgatgctc gggcccacga ggccgcaatg gtcgtggatg atgtcgaact
     2041 cgtctttgtg cttgtaggcc gaaaccgcat gcacggcttc gaaccagatc tgtccgagca
     2101 tggtcccgcc cggcggctcg tcgaactcgg agacgagctt cgctttggtc gtcgaactcc
     2161 cgctggcgaa tagcgtgacg tcgtggccgc gctcggcgag cccgtcggcc aggagcgcca
     2221 cgacccactc gatgccgccg tacccggcgg gcggaaccga gaaccagggg gggcagatct
     2281 gggcgatacg catgatcgca gcataccgag ccgcgtcccg gccgaccgtg ctcacacgct
     2341 aggcgccgtc cgccggcgag caagatcatt ttgcgccgga cgtcggagtc gatcgagccg
     2401 aagacggttt cgaccggcag accgacggcg cgggcaaagc gagcgtggac tagtcggagg
     2461 gacgcagcag cgccagagga agcgcggcaa gcgttcggtc gagcgcgagg cgtccacccg
     2521 gcgtctcggc cgtccggccg gagagcgcat cccgccatcg gcgcggcgcg ccgcgcggca
     2581 gcgtcacggc cgtgtcccgc caggtcgcgc ggccgggaag gcgggctccc gtcacggctg
     2641 ccaggaatcg cggtgcgatg gcaacggccc atcgcccctc gtgctcgcga gcgacggcaa
     2701 cggcctggcc ggcccgcagc ccgtccgcga cgagcggcac ccaccggccg cgcgcgaaca
     2761 gatccggatc gtcgcggcgg acgcgcagcg cgcgcgcaat gacggccatt ttggtcgtct
     2821 cgtcgcgcgg gtcgaaggtc gcggcgcgct cggcgcgctc cagcagccgc gcgcgacgac
     2881 gaaagtcgac gcggccgcga ttgtcgggat cgacgagcgt gagcgaccac agctcggtgc
     2941 cttggtagaa gtcggggaca gccggagcga cgactttgag cagcacctgc gcgagcgagc
     3001 cgatcgcgcc gtgcaaaccg acgatgcgca gcaggcggtc gacgtcgcga gcgaagacgt
     3061 cgtcggcgac gacggcctgg acccagcgcc gcagcgcacg ctcgtgcttg tggtccggct
     3121 tgcgccacga agagtgccgc ttggcctcgc gcgccgcctt gcgcgcgtag tcggcgacgc
     3181 gcgccgcgag acgctgccgc gagccgcgca acggccacgc gccgacgata gtttggtaga
     3241 gcaggattcg ctcggcggca tccggggact cgtgctcgcc ggcgttcccg acgaaccggc
     3301 ggtgtcgccg ctcccatcgg tccagcagtc gcgtccattc ctcggggatc tccgacagca
     3361 cgttcagacg ggcgcgcacg tcctcgccgc gcttggtgtc gtgcgtggag gtcgcagcga
     3421 gcgacagcgg atgggtgtga agccgcgctg cgttgaagcg ggaaaagaac gcgagatcgg
     3481 tcgcgacgtc ggcgcggccg cccacctcgt tgagcgaggc gagcggggcg tgacggtaca
     3541 gcgccgtgtc ctcgaggccc ttggccatcg ccggcccgct gagctgttgc cagcgggcga
     3601 ccagatcgcg ctgcacccgc atcgagccgc cgtgctcgag cagcagcgcg gcgcgcagca
     3661 cgcgcagcgc tgcggcatcg gcttcgccgc gtgcatggcg aacggcccgt tcgatcagcc
     3721 gccggtcctc ggtttcggga tgggtggacg cgacgtaggt ccggtagacc gggagatgca
     3781 ccgtcaccgc gacgacggcc cccgcgtagg actcctcatc gatcgagtcg tcgaggctgc
     3841 ggagcaggcc ggcgagcgtc tcggcctcgg ccggaaacag cgtctcgagt gcgttgcgct
     3901 tgccgtcgtg cacgatctgc tcgaaggggg tgcggtcccc ggtgcggcgc atccagtcgg
     3961 tccgcagcgc ttcgaacccg gcgcgatcca cgaacacgcc gttgagcgcg ttgaggaact
     4021 cgtagccggt ggtgccgtcc accggccagc cggcgcggat gggctcatcg gcctcgagga
     4081 tcttctcgat caggatccag gcatcggagc cgagtgcgcc gcgcagccgg cgcagcgccg
     4141 cggccgggtc gcgcagtccg tcgatgtgat cgaggcgcag cccgtccacg agcccgtcgc
     4201 gcacgaggcg gatagccaaa ccgagaacgt cgtcgaacac gcgcgggtcc tcgacccgca
     4261 cgccggccag gtcggtgatg tcgaagaacc gccggtagac gatcttgcgg tcgccgcccc
     4321 gccaggaggc gagccgccac ggctgcgcgt ccaagaacgc acgcagccgg tcctcgccgg
     4381 gccgccgccg caatccgctg ccgtttcgct cgaacaggat cgtccacgtg cgcggcgcgg
     4441 tcggcagcgc caggtcgccg tagcggacgc ggaccgcgcc gccggcggtc gtcaccttga
     4501 tcgtgcgcgc acgcagctcg cgttccagcg gatgcttgag caccggcagc acgacggcat
     4561 cggcagcgcg ctccaggtcc aggtcgaagc ggaacgcgct ctcggcgtcg atgccgttga
     4621 cgagcaggtc ttcccaccac ggattgtccg gcgaggccgc gacgtggttg ggcacgatgt
     4681 cgagcaggat ccgcagcccg tgcttgcgcg cggcggcggc gagcttgcgg aagccctcct
     4741 cgccgccgag cgccttacga atcgcgcccg gatcggtgac gtcgtagccg tgcgtgctgc
     4801 ccggccgcgc ttccgtgatg ggtgacagat acagatgcga gacaccgagc cgctcgagat
     4861 acggcaggat cgcacgcacc cgcgcgaacg tcagatccgg accgagctgc aggcgatagg
     4921 tggcgaccgg cgtgtcacgg ctcatcgaag cgcaggaaga tcagcgagtg cgcggccagg
     4981 ttgaccgcat cggtcttgac ggtccgttca ccgggccgcg cggtgttgag cacctcgatc
     5041 cactgaccgg ccccgacctt gggcagggtg aagtgccgcg agcgcggtcc gccgttcagc
     5101 agcagcagca ccgtctcgcc cgaggcgggg cggccttgct cgtcgacctc gtcagtggcg
     5161 cggccgtgga tgagcatgcc gatcacgtgg ttggagccgt cgtcccagtc gtgctgctcc
     5221 atctcgtttc cgtcgggccg cagccaggtc agatccttga agccctggtc cgagatggtg
     5281 cggccggtga agaagcttcg ccgccgcagc acgggatgcc ggtgaaacag ctcggtcagc
     5341 gtgcgtgtga actcgagcag ctcgcgatcg tcctggtcga ggtcccagtg gatccaggtc
     5401 ttgtcgttgt cctggcagta gccgttgttg tttccccgct gtgtgcggcc gacctcgtcg
     5461 ccgtgcgaga gcatcggcac gccttgcgag agcatcagcg tcgccaggaa gttgcgcttt
     5521 atgcgctcgc gcatccgcag gatgcgtgtg tcgtcggccg gtccctccgc accccagttg
     5581 tggctgttgt tatcgtccgc gccgtcacga ttcccttcac cgttgtcctc gttgtgcttc
     5641 tgctcgtagc tgaccagatc gtgcaacgtg aagccgtcgt gacaggtgac gaagttgatc
     5701 gaagcgtagg tgcggcgccc gctcgcctga taaagatcgc tcgatcccga cagacgcgag
     5761 gcgacctccg ggatcaggcc cggatcgcca cgccagaact tgcgaatcgc atcgcgatac
     5821 tttccgttcc actcggccca gccgaccgga aagtttccga cctgatagcc accggggccg
     5881 acgtcccacg gctcggcgat cagcttcacc tgcgagatcg tcgggtcctg ctggatgatg
     5941 tcgaagaacg tgccgagacg gttcacctcg taaagctcgc gcgcgagggc cggcgccaga
     6001 tcgaagcgaa acccgtccac gtgcatgtcg gcgatccagt accgaagcga gtccatgatc
     6061 agctggatcg tgcgcggatg aagcatgttg agcgagtttc cacagccggt gaagtccatg
     6121 tagtagcgct catcctcggg cgtgagccgg tagtagctct tgttgtccac gccccggagg
     6181 gacagcgtcg gacccaggtg gttgccctcg ccggtgtggt tgtagacgac gtccaggatc
     6241 acctcgatcc ccgcgcggtg gaaggtcttg accatggtct tgaactcgtt gacctgctcg
     6301 ccgagcccgc cggtcgcgta gcgcacgtcg ggggcgagaa acgcgatcga gttgtagccc
     6361 cagtagttgg tcaatccccg ctcgtagagc tcgcgttcga cgacgaactg gtggatcggc
     6421 atcagttcga cggtggtgac gccgagcgag agcagatggt tgatcaccgg ctcctgcgcc
     6481 atccccagat acgtgccgcg cagctcgtcg ggaatttccg gatgcagcat ggtcatgccg
     6541 cggatgtgag tctcgtagat gaaggtacgg ttccacggaa ccagcggccg gcggtcgtcg
     6601 ccccagggga acgccgggtt gatgaccacg cacttcggca acgccgccgc gctgtcgcgc
     6661 tcgtcgggca ccagatcgtc ctcgccgatg cgatagccga acaaggtgtc gtcccacttg
     6721 acggtgccgc tgattgcctt tccgtaggga tccagcagca gcttgttggg attgaagcga
     6781 tgcccctccg cgggagcgta cggcccgtgg atgcggtagc cgtagagctg gcccggccgc
     6841 gcgtcgggca gatagcaatg ccagatcaga tccgttcgct cgcgcaacgg gacgcgcagt
     6901 gtttctttcg gatcatcggg cgcgtcgaat aggcatagct caaccccttc ggcatgctcg
     6961 ctgaagatcg cgaagttgac gccttctccg tcccaggtgg caccgagcgg atatggcgag
     7021 ccgggccaaa cgcgcatcga ctagtccttc ctcgacagca acagaaacga gtgcccgggc
     7081 cgggtgatcg tctcgccgga tcgcaccgtc gcgttcgtgg ggctgccggg gccggcccac
     7141 cgcgcgtcgg ccgaatccag ctcgacggtc cactcgccgg cgagccgctc gctcgcctgc
     7201 tccggtgacg tattcaatac cgcgagaact tcgtttccat gcgcgcgacg cagcacggac
     7261 acgagcgggc cgcgcgcggt cgcggcggcg ggaacacccg tcgccatcgc gggcatgcgg
     7321 cggcggagcg cgatcagatc tcggtaccag tcgcgcagcg cgcgtccgcg cggctcgtca
     7381 gccagcgagc ggttgagctt ggagcgctcg aaggtttccg ccgattgcgg gtcgggcggc
     7441 tctccttgcc agccgaatga cgcgaactcg cgttttcgtc cgtcacggac cgctttgatg
     7501 agcttcgtct cgccgtggct ggtgaagtac aggaacgggt tggtctcgcc gtactcctcg
     7561 cccatgaaca gcatcggcac gaacggcgag tacagcaacg cgccggcggc cagcttgagc
     7621 gcctcgaagt ccaccagggc cgcgagccgg tccccgagca tgcggttgcc ggtctggtcg
     7681 tgattctgga tgcacaccac gaagcgctcg cgcggaagct cgctggcggg cgcgccgtgg
     7741 cggcggccgc gaaacacgga ccggcggccg tcatagacga agacgtcggt gtaggccttg
     7801 gcgagatcct gcggcgatcc gaagtcggcg taatagccgt cgcgctcgcc ggtcagcaat
     7861 gcatgcacgg catggtggaa gtcatcgctc cactgcgcgt ccatgccgag ccccccgagt
     7921 tcgagcgcgt gcaccatctt cggatcgttc tggtcggtct cggcgatgag ctgaagcggg
     7981 cggcccagct cggcttccca ttcacgcttg tgcatcgcga gctcccgcag gaacggcccg
     8041 gccgagaagt ccttgatcgc gtggaccgcg tcgagccgga gcgcatccat gtggtactcg
     8101 aagaaccaca tgcgcgcgct ggcgatgaaa aaagcgcgga cctcgtcgct gccgctgccg
     8161 tccacgttga tcgcgtcgcc ccacggaccg tggtattcgt cggtgtggta gggcccgaac
     8221 tcgcgccagt gcgccccttc gggtccgaag tggttgtaga cgacgtccat gatcacggcg
     8281 atgcccgcgg catgacatgc gtccacgagc gcgcgcagcc cctcggggcc gccgtaggtg
     8341 ttttgcggcg cgaacgagaa gacgccgtcg tagccccagt ttcgctcgcc cgggaactgc
     8401 gagacgggca tgatctcgat cgcggtcacg cccaggtcgg tgagctcttt gagccggtcg
     8461 gcggcggcgc ggaaggtccc ttccggagtg aaggtgccga tgtgggtctc ggagatcacg
     8521 tagtccttca gcggcaggcc ccgccatccg tcatcggtcc acgcgaaagc ggagggatcg
     8581 acgacctcgc tgtcgccgtg cacgccatcg ggctgcgagc gcgaggccgg gtcgggccgc
     8641 ggctcgccgt cgtcgagcga gtaccggtag cgcgttcccg ggcgggcgtc cgcggcgagc
     8701 gcctcgaagt agtcgccgtc gcgcgtcatc gacacggagt ccgcgccggt gatgcgaagc
     8761 tcgacctgct cggcgcgggg cgcccagacg cggaatcgca cgccgcgatc atgcggcagc
     8821 gcgccgatca tctcatcgcc gttcatcgct cttgagcatc agcaccgaaa gcggggggag
     8881 cgtgagcgtc agcgcgcgag gccggccgtg cgcgccgacc ggcgaggcct ctttgccgcc
     8941 ggcgttgcct tgaccgctgc cgccgaactc gcgcgcgtcg gtgttgagga tctcacgcca
     9001 ctgcccgccg cgcggcacgc ccaggatgta attgtgccgc ggcaccggcg tcagattcgc
     9061 cgccaccagg atcagatcgt cggtggagcg gccgcgacgg atggaggcga tcacgctgtt
     9121 ctcggcgtcg gaggcgtcga cccactcgaa cccggccgcc tcggcgtcca tctcgtgcag
     9181 cgcgggttcg cttcgataca tccggttcag ctcgcgcacc agccgctgaa cgcccgcgtg
     9241 cagctcgtag tcgagcacgt gccagtcgag gctggcctcg tggttccact cgccgcgctg
     9301 gccgaactcg gcgcccatga acagcagctt cttgccgggc gtggcgtaca tcagcccgta
     9361 gagcgcgcgc aggttggcga actgctgcca ctcgtcgccg ggcatcttgc ccagcagcga
     9421 gcctttgccg tgcacaacct cgtcgtgcga cagcggcagc acgaagtttt cctgccaggc
     9481 gtagagcgac cggaacgtga gcatgtgctg gtggaagcgg cgatgcaccg ggtccttctt
     9541 gaagtactcg agcgtgtcgt gcatccatcc catgtcccac ttcaggccga atccgaggcc
     9601 gcccacatac gtcggccgcg acaccatcgg ccatgccgtg gactcctcgg cgatggtttg
     9661 cacgtccgga tgcgccgtgt agacgtcctc gttgaagcgg cggaggaagg cgatggcctc
     9721 caggttctcg cggccgccga actcgttggg gatccactcg ccgtccttgc gggagtagtc
     9781 gagatagagc atcgaggcga ccgcgtccac gcgaaggccg tcgatgtgga agcggtcgag
     9841 ccaatggatt gcgctcgaca tcaggaaggc gcgaacctca ttgcggccgt agttgaagat
     9901 gagcgactcc cagtcgggat ggaacccctg ccgcggatcg gcgtgctcga acagatgcgt
     9961 cccgtcgaaa tatcccaggc cgtgctcgtc gctcgggaag tgcgacggaa cccagtccag
    10021 gatcacgccg atgccgcgct gatgcagcgt gtccaccaag tgcttgaagt cctgcggcgt
    10081 gccgtatcgc gcggtgggcg cgaaatagcc gctcgcctga tagccccacg atccgtagaa
    10141 gggatgttcg gtgaccggca tcagctcgac gtgcgtgaag cccatctcct cgacgtagtc
    10201 ggcgagcggg cccgcgagtt cgcggtagct gaggctgcgg ttgccgtcct cgggcgcacg
    10261 ccgccacgag cccagatgca cctcgtaggt cgacatcggc gaggcgaggc cgccgcgcgg
    10321 tccgcggccc gccatccatt caccgtcgtt ccaggtgtag tcgtcgtccc aaacgatcga
    10381 gccggtcttg ggaggcgttt cgtgatgcac cgcgaacggg tcggccttgt ccacgcgata
    10441 gccggtatga cggctatgga tccggtactt gtagatcgag ccttgtccga tgccttcgac
    10501 gaaagtctcc cagatgccgg agccgccacg cgcgctcatc ggatgggccg tgtcgtccca
    10561 gccgttgaag tcgccgatga gcgagacccg ctcggcgttg ggcgcccaca cgccgaagtg
    10621 cgtgccgccc tgcgtcatgt gcgagccgag cttctctccc agcttgtagt gcgttccttc
    10681 gttgaacaga tagatgtcgt cgtcggtgag cggactggtc gtctctcgcg cctctcgtgc
    10741 ggccatctca tctcacctcc gtgtcagcgt ccggatgcgg gcggctacgg ccgggtcgcc
    10801 gcggatctcc tcgagcgtat agcgaagctt gtggcgccag ttcggctgct cgtcgctggt
    10861 ggtgcccgga acgttctgct ggcgttcctc gagccacaag tcctcgatcg acacgcagcg
    10921 catccgggcc cggccgcctg ccagcagcgc gagcgacgcg tcgagcgccg cgtccgcgcc
    10981 gcggcccttg agcagcttcc tgccgcgaag cgacgccgag agtcgtttcg tcgtcttcgc
    11041 cctgcgcgtt cgttcttttt cggcttgcgc gggcgtgaac aggccgaggc cgacgcgatc
    11101 atcgatgtcc ttgccgttcc agaacgaggc gaacgtcggc atgtcgtgcg tgttgaggcc
    11161 ggcgagcgcc cgcgcgggga tcgggttcgg aacctgcttc ggagaatccg gcagctcctc
    11221 ctgcagcacg tagctgcgca gcaggttgtt gcgtgccatc gcggtgcgca cctcgcccgg
    11281 cacggtcccc aagtcctcgc cgacgatggc cgcccccgcg cggtgcgact cgagcgcgag
    11341 gatcgcgtac cactcgtccg ggttcgagcg aacgtagacg ccgtcggtcg cgtcgaatcc
    11401 gtccggcacc caaaacagcc ggtgcaggct catgacgtga tcgatgcgaa ggatgcgcgc
    11461 gtgacgcaac agcgcgcgca gcgactcgat cacgtacgag tagccggtct cgcggatccg
    11521 ctcgggatgc agcggaggga atcgccagtt ctggcccttg gtgaagaacg agtccggcgg
    11581 cgcgccgccc gagaccccga aggcgaacac ctcgccgtag cgccacgtgt cgaacccgtc
    11641 gggatgcacg cccagcggaa ggtcgaagta cagatgcacg cccttgtcgt cggcctcgcg
    11701 gcccgcgcgc gcgagttgct cgttcatcaa ccattgcgcg tagacgtgat atttctcggc
    11761 atcgcctccg cgccgcagct tcgcggtgcc cggccaggcc tgccagggca ggccgctgcg
    11821 ctcggtctcg gcacggaacc gtgcgtattc gagcgtaacc gggttctcgc gcagccacgc
    11881 gtccatctcg cgccgccgct tgccgctcag cgacgcggca cacgcctcca ggacgcggcg
    11941 cttgacggcg gagaccccgc ggtaatccac gtcctgcttt cgccgcagtc cgcgaatctt
    12001 gggcgcgagc tccttggcga gcgcgcgcgc ttgctcggag tccgcgaact cgggaacggc
    12061 gtcgatgtcg aggtagatct cgttccaatg cagccggctc accggcgcat acgggctcgg
    12121 gtcgaacggc tcgtccatga aggccgacaa gatcggcagc gtggccacca gcgagccgcc
    12181 gagctcggaa acgacgtcga tcagcccggc gaacgacccg agatccccgc agccgtagtc
    12241 gcgctcgccg cgaaccgcat agacgggagc gaacacgccg gcggctcgcg gcccgtcgtg
    12301 catcgcgggc ggtgcgacga tcagccgcgc ctccgcggcg gcatcggcct cgacgcgcag
    12361 ccggtgatag ccgaacggaa ggttgcgcgg cagcggga
//
LOCUS       DATLFO010000291        17696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_96704, whole genome shotgun sequence.
ACCESSION   DATLFO010000291 DATLFO010000000
VERSION     DATLFO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96704"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VM841_11745"
     CDS             <1..1524
                     /locus_tag="VM841_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL90894.1"
                     /translation="TDNRGTLTTGIDGLSAGGGTALYDGVTLAASALAKEPGQKTIVV
                     FSDGRDTARATTIATAIDAAKKAGTSITAVALTTADTDRGALQALARDTRGSLVEVGA
                     SAGLAQAFSAVATELSNQYVFSYTRSGGDKQLELMVALGDARDAIVVANPRTAPSQPP
                     TPVPLPAAPPPPVAALAGTPGLLVGLVAAFGFVALLGSILFRKAAPTPGMQALTRGIK
                     ATERRIRPRREETEDEPAVSGALRELRRAADGVMRRVPRLQGFERSTQRMLERASWPL
                     RASEFLMLQAGAAAGTFVLARALLGRWAIAVVLAGAAGLVPRGLLSNRIAKRQAAFLE
                     QLPDTLTLLAGTLKAGYGFMQALDVLVQETPQPTAGEFSRVLTEARLGMPMDGALNAM
                     AERTGSEDFRWVVLAINIQRQAGGNLADLLTTVAQTLRDREAVRRQIKTLSAEGRLSA
                     IILGALPFVIAGYISVVNPGYLSGLFSSSTGKIMIVAALGLMGLGIAWIRKIIRIDV"
     gene            1537..2466
                     /locus_tag="VM841_11750"
     CDS             1537..2466
                     /locus_tag="VM841_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVL90895.1"
                     /translation="MDVGTIGLLSAFGAAFLGFVAAGDVFLERHQMYKRLRSVREIQL
                     SPTDVRHQELAKSPTERLLVPILKKVGNRMRRMAPASVGERLERELALAGSPPGWDAA
                     RILASKLLSLGIGVVAAFGLAVAGSLQPVQGMALAAIGAVVGYYLPEWLLRAKSGRRQ
                     DALQRALPDALDLLSITVEAGLAFDAALDRVAQHAGGPLGEEFNRVITEMRIGTSRAD
                     ALRGLGERTTVPELRSFVMAMVQADVFGISIARVLQVQASEMRAKRKQRAEERAQKVP
                     VKIVFPVLACIFPAMFLVILGPAVQQIYRSIIQ"
     gene            2841..4013
                     /locus_tag="VM841_11755"
     CDS             2841..4013
                     /locus_tag="VM841_11755"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL90896.1"
                     /translation="MARTAARPPLLSRSYVLVCAATLLFYLATQMQIPLYPPLLRERA
                     GASASVVGLFIGAYTVLAVLTRPMVGRELARRPRRLFMAAGGAVTVAATIGYWLAGSL
                     AALIAIRVLHGSAVALFYTAASTTVADLAPAERRGEALSLFSMFLYLGLAAGPAIGLA
                     LHRAAGFGAMFAVSIGFAAACAAIALAIREPRLHSPEAREIERHPLVHRAALLPGAVL
                     ACAAVGYAAGLNFTVDYAAEHRIGGSAFYFPVLALSVIATRLVAGRASDVLGRRRVAA
                     PGLALFAAAMATEALARGPAGLLASAAMFGIGFGMFFPTMMAFTTDRVSARERGSAMA
                     TFTASFDVGFGLGSPLLGAIHGAAGYPVMYAVAAGMAALGLLVLASPSMRSGARTG"
     gene            complement(4220..4915)
                     /locus_tag="VM841_11760"
     CDS             complement(4220..4915)
                     /locus_tag="VM841_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009409159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90897.1"
                     /translation="MRILVIEDDPALALPLRKSLEREGYEVRLCDEGGQGVDVARTWH
                     PSVVLLDVELPDGNGREVAQRIRTFSPVPIIMVTGRGQTQDKIRGLDAGANDYVVKPF
                     DIDELLARIRAATRPAVASATAADPTASTVALADLRLDMPARRAYRGEEDLGLTVKEF
                     EILRVFAFRPGEIIRRDEISRAVWGTSPSEGGNTIDVHMSWLRKKLGDDPASPRYIET
                     VRGRGFRFRAPAS"
     gene            complement(4949..6925)
                     /locus_tag="VM841_11765"
     CDS             complement(4949..6925)
                     /locus_tag="VM841_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HVL90898.1"
                     /translation="MRRPGRKSGIWAVTALFAAIGAALWLAGESGLGAYSSHLHVPWW
                     VLAAMFAFAEINVIHIQPRRETQTFSFSEIPIVLGLFFSSPAELIVGQVLGAGIALIV
                     HRRQPAVKWTFNLAQLWASVALAVVAFRVLARPDGVLGGAEIAAAFAATGVAIATGVL
                     AVMAALTVTEGVPGGRTFFRHAWFGVLGTLTNTSLALMAVTLLRQDARTGWLLIVPVV
                     AFYFAYRGYASHRMQHETLSTFSEATRTVQSSLDLEPAMRTVLERAREIFRADIAYAT
                     FLPQEPGEPAIQARIGFEGQEEVLATVEADPTEGIWARVLAEGRGVVLPGPIGNERLH
                     EHFAAQGIRDVVCAPLIGRDGAIGTMTIANRRGDVATFTEDDLRLFEAFVSHIGSALE
                     NVRLVARLQMSLGDATVRDQLKDEFLGTLSHELRGPITSMMANIAMLQAGDLTSEEAG
                     AGLETVANQAQHLRALVEDLLMDVRLSGNAVTPVPMTIRVSDLLDRVAAASRPWARNH
                     RIVCQEFGEIPPMMTDPDMVHRILTNLVGNAAKNSPDGSTVTISARRDADSVVFTVED
                     EGVGIPREIQERIFERFFHLDRTPGRGRTGMGLGLSICMRLAGVLGGRVWLARSEPGA
                     GSEFALRVPLRVVADAAVHVSGGAFPSAPSHTLN"
     gene            complement(6933..8696)
                     /locus_tag="VM841_11770"
     CDS             complement(6933..8696)
                     /locus_tag="VM841_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90899.1"
                     /translation="MISPVHRIARSLLSVVVLVAATAAVVAPAAAAIGVVVRAEPGAD
                     VHVARTLHRMGGEIGTRLPLIDGFAATIPASSVDRARAIPGVVAVTPDASLRLMHSVD
                     GFDGATDPGSSFNLAKTIRSHDLWKSGITGRGVDIAMIDSGVLPVNGLTYSGKIVNGP
                     DLSFESQASNLRFMDTYGHGTHLAGIIGGRDDAATSLQAMTGHDTFTGVAPEARIVNI
                     KVANAHGATDVSQVLAAIDWVVQNRNAHGLNIRVMNLAFGTDSTQDRRLDPLSYAVEV
                     AWRRGIAVVVAAGNDGRNTVGLANPARNPFVIAVGADDSKGTYEVEDDTIPAWSSRGD
                     GVRNPDLVAPGKSLVSLRAPGTYIDQQFPGGRASARFFRGSGTSQAAAVVSGAAALLI
                     QQRPSITPDQLKALLTSTATSVPLAPATAQGAGLLNLKSAAAAPTPNVTQTWTLSAGT
                     GSLEQARGSAHVVDENGNVLEGEQDIFGKTWDGTTWSAGAWNGTTWSGGEWLGTTWSG
                     NCLCVQTWSGMGWAGTTWSGTTWSGTTWSGTTWSGTTWSGTTWSGTTWSGTTWSGTTW
                     SGTTWSGTTWSGTTWSSAAWE"
     gene            9279..9950
                     /locus_tag="VM841_11775"
     CDS             9279..9950
                     /locus_tag="VM841_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVL90900.1"
                     /translation="MNVAVLGTGSVGRTVAGTIGSLGHEVRIGTRDVEALMARTDDAP
                     TFAEWITTVTSVQAMTFKDAATFGEIVFNATSGTASLEALRAAGAANLAGKVIVDIAN
                     PLDFSKGFPPTLSVCNETSLAEQIQAAFPDARVVKTLNTVNASVMVDPVSVGGGEHDM
                     FMAGDDDDAKRTVESILRDWFGWTRVTDLGDITNARGMEMYLPLWVRLYGAAGTGAFN
                     IAIVR"
     gene            complement(10043..12136)
                     /locus_tag="VM841_11780"
     CDS             complement(10043..12136)
                     /locus_tag="VM841_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HVL90901.1"
                     /translation="MRRSLILAVVTSLLIVLPVVSQAADDVSIRVLSNRADLISGGDA
                     LVEIVLAGGVDPAGVTVDVDGRDVTSSFAVRADGRFLGRVEGLALGVNNLNARAGLAG
                     ATIPITNHAIGGPVFAGTQIQPWLCTTQSNGLGVAQDAQCNAPTVHTYHYRSTNPART
                     GFLAYDPNNPPADVATTTTDQGEVVPYIVRRERGTADRGIYDIAVLFDPARGWEPWAR
                     QEGWNGKIHYVFGASCGTIHSQSSPANVLDDRALSRGFMVVNSSMNNLGNNCNTVVSA
                     EAAMMLRERVIERYGEIRFVIGSGCSGGSIGQHMVANGYPGLIDGIQPNCSYEDNWST
                     GMEVVDCHLLLNYYVRTSPHLWAAAQQQAFVNGHQSPTSCAAWEALFAPVADPKGGCG
                     LPPEQEYHPQSNPGGCRGSLADYYKPVLGTRAQDGFAPLPYDNVGVQYGWHAVNSGLI
                     TAEQFVDLNEKIGAVDIDFNFAPGRRTADPGSINTLERSGQINDGAQLDEVAIIDLRG
                     SSNEEIHTDFHTYSMRERLKKANGHADNHVIWSSHVPLVGDPVWATESFLLLDRWLTA
                     VEADTSALPRAEKIRRNKPADAVDSCWVAGQRVSDATLCRTVVPYYGAPRIAAGGPAT
                     HDIAKCALKPLDRRDYRVSFTDGQWSRLQAVFAGGVCDWSKPGIESATSTPWMTFAGG
                     PGGQPLPAPPVSQSL"
     gene            complement(12235..14382)
                     /locus_tag="VM841_11785"
     CDS             complement(12235..14382)
                     /locus_tag="VM841_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HVL90902.1"
                     /translation="MKATTGHEVPHEQHASGEHDGDAAGAYDPSAPHGGRAAPAGHGG
                     HEAPAGHGGQGGHAGHAGMSMAEMARDMRNRFLVAAVLAIPITLFSRIGRDVIGFSAA
                     APLGMSDEWAQMLLSIPVVFYSGWIFFSGAAAALRARTLDMMVLVAVAIGAGWIYSVG
                     VTLTGGGEVFFEAATVLAAFVLLGHWFEMRARGGANDAIRRLLDLSPPAATVIRDGLI
                     VDIPTAEVAAGDLLLVRPGSKLPVDGVVEEGESSVDESMVTGEAMPVGKRSGDEVIGA
                     TINRNGTLRVRATKVGADTALAQIVELVRAAQESKAPGQRLADRAAFWLVLVALIGGA
                     ATFLTWWGVGAPVTEAMLFAITVVVITCPDALGLATPTAIMVGAGLGAGRGILFKNAM
                     AIEASARIDTVVLDKTGTLTEGKPVVVAVEPRGIDENDLLRLVAAVEAESEHPLAEAI
                     VRHAIGHGIPAVRAARFEAVPGHGAVATVDGRRVAVGNDRLMKQVGAAAIEGSDQTEA
                     GRTVVGVAVDGTYSGSIAIADAPRPTARAAVRALRDAGIEVVMLTGDRQATATRIAAE
                     LGIDRVIADVLPGDKAAQIVALQREGKRVAMVGDGINDAPALAQAEVGIAIGAGTDVA
                     IETADVVLMRSDPLDVATALTIGRGTLRKMRQNLGWAIGYNALALPIAAGVFVPAFGL
                     MLRPEIAALSMSGSSVLVAVNALLLKRLDLPRA"
     gene            14647..>17696
                     /locus_tag="VM841_11790"
     CDS             14647..>17696
                     /locus_tag="VM841_11790"
                     /inference="COORDINATES: protein motif:HMM:NF017368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HVL90903.1"
                     /translation="MAQRRATVRDRALRIASALAVAAALFPGTAPARQATHARYDIDR
                     FREAAAAAVTRVGAPPAAAASYFDDHTFSFAHTGEDPVTLSQPDGHRFRVRQRPASDG
                     GGLETVEGELVVRGDDEIWRYARWGETTRLVATDAVVGRDPAPPVSHATRYSPAMKKA
                     LTEDAVRREEMRRYTATRARAVADAAAAAGEPVVLRIPVLLFKINNRDFLPGTTPASV
                     EALIDGHGTSMHGTLAELYEEQSHGRLILEFDVFGPYDVSVSNNPQNDCWYGTLGGPV
                     FGDVLGIGGLGAKGMAQEVVPQADPDVDFSRYDNDGDGYVDFTAIMHSGTAYEVTGSW
                     CDVWSHYWSGFGPPAGLPPDPTLTTDGVVVGSVLTVPEVAPFTGVFGHEIMHALGEPD
                     YYGTQGTSGTGDWDLGAGGGYLGQPSQSNPAHLNPVVKINMGWVEPEIVDATTYDVEI
                     RARATHPDAVAIPLTVEPAGTRADQLCGAQAIGGFPEQNRRFRQPNGDCIVEGLLIEH
                     VSQSAGRYETAPGGDCRFVPAHFDRQVYASGLAVWHFDFRSYTQLGNNNLGRPMLWLV
                     EFDRRDDTQEIRSGVTRGEPTDLYWGDPVGISTATGSRVGAIAPPPPGSPYTASAPLP
                     GAAQQTPDWTVPPEADSQTMTLTLTWDTQNLDDWDLHLYKEVESDGETYWEEVNAAAG
                     PGARGPEVVDHPVAAGEKYFALARNWASVSPEARIEVVYGARGSGSKASTLTNNGVGL
                     GWQITGMSSPSSSGTAHAAQRPGEPITLDIVRHDGTTVDVSPDFLTPAGGAVPVIAGK
                     PIEMSTRLYNHGGKAVDQATISLFGVDPSTPGATPLSTVTRSLSAFDRQDVTFTFKAA
                     AGTRTYYVRTVAAGDVHPSNDVVATEISADPASANVLVVDADRGFTFEEPVEAVLQGL
                     GVPYHVVRGIPTLTTLRAYPLVVWLTTSVSGPDGIFHYSSHEALWDYLAGGGRVWMQS
                     TRALATSWIFPEFFGIGSSWFAPDSQGRALPKSATTLPAIDLGTL"
BASE COUNT         2678 a         6223 c         6081 g         2714 t
ORIGIN      
        1 accgacaacc gcggcacgct cacgaccggg atcgacgggc tgtcggccgg gggcggcacc
       61 gcgctctacg acggcgtcac cctcgccgcg agcgcgctcg ccaaggagcc cggccagaag
      121 acgatcgtgg tcttcagcga cggccgcgac acggcgcggg ccacgacgat cgcgacggcc
      181 atcgacgccg ccaagaaggc gggcacttcg atcaccgcgg ttgcgctcac caccgcggac
      241 accgaccgcg gcgcgctgca agcgctggcg cgcgacaccc gcggatcgct cgtcgaggtc
      301 ggcgcctcgg ccggcctggc gcaggccttc tccgccgttg cgaccgagct gtcgaaccag
      361 tacgtgttct cctatacacg cagcggcggc gacaagcagc tcgagctgat ggtcgcgctc
      421 ggcgacgcac gcgacgcgat cgtggtcgcc aacccgcgca ccgcgccgtc gcagccgccc
      481 acgccggtcc cgttgccggc ggcgccaccg ccgccggtcg ccgcgctggc cggcacgccc
      541 ggcctgctgg tcgggctggt cgcggccttc gggttcgtgg cgctgctggg ctcgatcttg
      601 ttccgcaagg cggcgcccac acccggcatg caggcgctca cgcgcggcat caaggcgacc
      661 gagcgccgca tccgcccgcg tcgcgaagag accgaggacg agccggcggt ctcgggcgcg
      721 ctgcgcgagc ttcgccgcgc cgccgacggc gtgatgcgac gggtgccgcg gctgcagggc
      781 ttcgagcggt ccacgcagcg gatgctagag cgtgcgtcat ggccccttcg ggcgagcgag
      841 ttcctgatgc tgcaagccgg cgccgccgcc gggaccttcg tgctcgcacg cgcattgctc
      901 ggccgatggg cgatcgcggt cgtgctcgct ggcgccgccg gcctggtgcc acgcgggctg
      961 ctttcgaacc ggattgccaa gaggcaagcc gcgttcctcg aacagcttcc cgacacgctc
     1021 acgctgctgg ccggaacgct taaggccggc tacggcttca tgcaggcgct ggacgtgctc
     1081 gtgcaggaga cccctcagcc caccgccggc gagttctcgc gcgtgctcac cgaggcgcgt
     1141 ctaggcatgc ccatggacgg cgcgctcaac gcgatggccg agcggaccgg cagcgaggat
     1201 tttcgctggg tcgtgctcgc gatcaacatc cagcggcagg cgggcggcaa cctcgccgac
     1261 ctgctcacca cggtggcgca gacgctgcgc gaccgcgagg cggtgcggcg gcaaatcaag
     1321 acgctctcgg cggaaggccg tctgtccgcc atcattctcg gcgctttgcc gttcgtgatc
     1381 gccggctaca tctcggtcgt gaatcccgga tacctgtcgg gcctgttctc gtcgagcacc
     1441 ggcaagatca tgatcgtcgc cgcgctcggg ctgatgggct tgggcatcgc ctggatccgc
     1501 aagatcattc gaatcgacgt ctaggaggcg ttcgccatgg atgtgggaac gatcggtttg
     1561 ctctccgcct tcggagccgc gttcctgggc ttcgtcgccg ccggcgacgt gttcctcgaa
     1621 cgccaccaga tgtacaagcg gcttcgctcg gtgcgcgaga tccagctttc gccgaccgac
     1681 gtccggcacc aggagctcgc gaagtcaccc acggagcggc tcctcgttcc gatcctcaag
     1741 aaggtgggga accgcatgcg gcgaatggcg cccgcatcgg tcggcgagcg gctcgagcgt
     1801 gagctcgcgc tggcgggatc accgcccggc tgggacgccg cgcgcatcct ggcctcgaag
     1861 ttgctctcgc tcggcatcgg cgtggtcgcc gccttcggtc tggcggtcgc gggaagcctt
     1921 cagccggtgc aaggcatggc gctcgccgcg atcggtgccg tcgtcggcta ctaccttccg
     1981 gagtggctgt tgcgcgcgaa atccggccgg cggcaggacg cattgcagcg cgcgctgccc
     2041 gacgccctgg accttctttc gatcaccgtc gaggccggcc tggccttcga cgcggcgctg
     2101 gaccgcgtcg cgcagcacgc cggcggcccg ctcggcgagg agttcaaccg cgtcatcacc
     2161 gagatgcgca tcgggacctc gcgtgccgac gcgctgcgcg gcctcggcga gcgcaccacg
     2221 gttcccgagc tacgttcgtt cgtgatggcg atggtccagg ccgatgtgtt cggcatctcg
     2281 atcgcgcgcg tgctgcaggt gcaggcgagc gagatgcgtg cgaagcgaaa gcaacgcgcc
     2341 gaagaacgcg cgcagaaggt gccggtcaag atcgtgttcc cggtgctggc ctgcatcttc
     2401 cctgcgatgt tcctggtgat cctcggcccg gccgtccagc agatctaccg ctcgatcatc
     2461 cagtgagaga gcacggggag agaggacggc ggttgcggcg accgcgatcg tgcgatcttg
     2521 gcacacgcac gcgaccgcga cacgcgtgca cccgctggtc gccgcaccgc cacactcccc
     2581 ggatcttccg ggaccgcgac cctgagcacg tgcatgcctt cgacatgcgc gggcccgcct
     2641 cgctcacatt cgcgtcacgg atccatgcac gcgcatagca cgctcggttg aacacgcagc
     2701 tgcatggtgc agcgggcggc acgcgcaagc agaaggtggc tgccggccgc aagggcggaa
     2761 cggcctccgc cgagaagtcc tagcctctca ccgggatcgg ctcggccgga cgacgggggc
     2821 gctctggtag cgtgccgcgc atggcgcgca ccgccgcccg tcccccgctg ctgagccgtt
     2881 cctacgtctt ggtctgcgcc gccacgcttc tgttctatct cgcgacgcag atgcagattc
     2941 cgctctatcc gccgttgctc cgcgagcgag caggggcctc cgcgtcggtg gtcggtttgt
     3001 tcatcggcgc ctataccgtg ctcgccgtgc tgacccgccc gatggtcgga cgcgagctcg
     3061 ctcgccgtcc gcggcgtctg ttcatggccg cgggcggcgc ggtcaccgtc gcggcgacca
     3121 tcggctactg gctcgccggc tcgctggccg cgctgatcgc gatccgggtg ctgcacggat
     3181 cggccgtcgc gctgttttac acagccgcat ccacgaccgt ggccgacctc gcgccggccg
     3241 agcgccgtgg cgaggcctta tcgctgtttt cgatgttttt gtacctcggg ctggccgcgg
     3301 ggccggcgat cggcctcgcg ctgcaccgcg ccgccggctt cggcgccatg ttcgccgtct
     3361 cgatcggttt cgccgccgcg tgcgccgcga tcgcgctggc gatccgcgag ccacgcctcc
     3421 actcgccgga agcgcgcgag atcgagcggc atccgctcgt gcatcgcgcg gccctgctgc
     3481 ccggcgccgt gctcgcctgc gccgccgtcg gctacgcggc ggggttgaac ttcaccgtcg
     3541 actacgccgc cgagcaccgc atcggcggca gcgcgtttta cttccccgtg ctcgcgctca
     3601 gcgtgatcgc cacccggctg gtcgcgggcc gcgcctccga cgtgctcgga cgccgccgcg
     3661 tcgccgcccc cggtctcgcg ctgttcgccg ccgcgatggc gacggaggcg ctcgcgcgcg
     3721 ggccggccgg tttgctcgcg agcgccgcaa tgttcggcat cggcttcggc atgttcttcc
     3781 cgacgatgat ggccttcacc accgaccgcg tctcggcgcg cgagcgcggc agcgcaatgg
     3841 cgacgttcac cgcgagcttc gacgtcggct tcggcctggg atcgccgctg ctcggtgcga
     3901 tccacggcgc ggcgggctat ccggtgatgt atgcggtcgc cgccgggatg gccgcgctcg
     3961 ggcttctcgt cctggcctca ccgtcgatgc gatccggcgc ccggaccgga taggccccga
     4021 aacgacgaga agaccgcccc catggccggt ctccttgtcg tttgcggggt attttcgttg
     4081 ccccccggga tatgaagagg cggatcccgg cccccgtcag cacgggatcc accacctcgc
     4141 tacctacgat acggacggcg gaaatgatct caagttaaag cattattaat gccgagcacc
     4201 gaccggaaaa gccggtggct cagctcgcgg gggcccggaa ccggaagcca cggccgcgca
     4261 ccgtctcgat atagcggggg ctcgcagggt cgtcgccgag cttcttgcgg agccagctca
     4321 tgtgcacgtc gatggtgttg ccgccctccg acggcgaggt cccccaaacg gcacgcgaga
     4381 tctcgtcccg gcggatgatc tctcccggcc ggaacgcgaa gacccgcagg atctcgaact
     4441 ccttgacggt caagcccaga tcctcctcgc cccggtaggc gcggcgggcc ggcatgtcga
     4501 gacgcaggtc ggccagcgcg acggtgctcg cggtcggatc ggccgccgtg gcggacgcca
     4561 ccgcaggccg ggttgccgcc cgaatgcggg cgagcagctc gtcgatgtcg aagggcttga
     4621 ccacgtagtc gttggcgccc gcgtcgaggc cgcggatctt gtcctgcgtc tgcccacggc
     4681 cggtgaccat gatgatcggc accggcgaga aggttcggat gcgctgggcg acctcgcgac
     4741 cgttgccgtc gggaagctcg acgtcgagca ggaccacgct gggatgccag gtgcgtgcga
     4801 catcgacgcc ttgcccgccc tcgtcgcaca agcgaacctc atagccttcg cgctcgagcg
     4861 acttgcgaag cggcaaggcg agagcggggt cgtcttcgat tacgagaatg cgcacgggaa
     4921 gccctccgcc gcggattgta cgcgagggtt agttcaaggt gtggctgggc gcgctgggga
     4981 acgcgccgcc ggacacatgg acggcagcgt ccgccaccac ccgcagcggc acgcgaagcg
     5041 cgaactccga cccggccccg ggctcagatc gggcgagcca cacccggccg ccgagcacgc
     5101 ccgccaggcg catgcagatc gacaggccga ggcccatccc ggtgcgtccg cggccgggcg
     5161 tgcggtccag gtggaagaac cgctcgaaga tgcgctcctg gatctcgcgc gggatgccca
     5221 cgccctcgtc ctcgacggtg aagaccaccg agtccgcgtc gcggcgagca gagatggtca
     5281 cggtgctgcc gtcgggcgag ttcttcgccg cgttgcccac gaggttggtc aggatgcgat
     5341 gcaccatgtc gggatccgtc atcatcggcg gaatctctcc gaactcttga cacacgatcc
     5401 gatggttgcg cgcccacggt cgcgaggcgg ccgcgacccg atcgagcaag tccgatacgc
     5461 ggatcgtcat cgggaccggg gtgacggcgt tgcccgacag ccgcacgtcc atgagcagat
     5521 cctcgacgag cgcgcgaagg tgctgcgcct ggttcgcgac cgtctcgagg ccggcgccgg
     5581 cctcctcgga ggtgaggtcc ccggcctgca gcatggcgat gttggccatc atcgaggtga
     5641 tcgggccgcg aagctcgtgc gagagcgtcc cgaggaactc gtccttgagc tggtcgcgca
     5701 cggtcgcatc gccgagcgac atctgaagcc gcgcgacgag ccgcacgttc tcgagcgccg
     5761 agccgatgtg gctcacgaag gcctcgaaca gacggagatc gtcctcggtg aacgtggcga
     5821 catcgcctcg gcggttcgcg atcgtcatgg tgccgatcgc gccgtcgcgt ccgatcagcg
     5881 gagcgcagac cacgtcgcgg attccctggg ccgcgaaatg ctcgtgcaga cgctcgttgc
     5941 cgatcgggcc gggaagcacc acgccgcggc cttcggcaag aacgcgcgcc cagattccct
     6001 cggtcgggtc ggcctcgacg gtcgcgagca cttcctcttg gccttcgaag ccgatccgag
     6061 cctggatcgc gggctcgccg ggctcctgcg gaaggaacgt cgcgtaggcg atgtcggctc
     6121 ggaagatctc gcgtgcgcgc tcgagcaccg tgcgcatcgc cggctccagg tcgagcgagg
     6181 actggaccgt gcgcgtcgcc tcggagaagg tcgagagcgt ctcgtgctgc atccggtgcg
     6241 aggcgtatcc gcggtaggcg aaatagaagg ccaccaccgg cacgatcagc agccacccgg
     6301 tgcgcgcgtc ctggcgcagc agcgtcacgg ccatgagcgc caggctggtg ttggtcaagg
     6361 tgcccagcac gccgaaccag gcgtggcgga agaaggttcg tccgccgggc acgccctcgg
     6421 tgacggtcag ggcggccatg acggcgagga caccggtcgc gatcgcgacg ccggtcgcgg
     6481 cgaacgcggc cgcaatctcg gcgccgccga gcacgccgtc gggccgcgcg agcacgcgga
     6541 acgcgaccac cgcgagcgca accgacgccc acagctgcgc caagttgaag gtccacttca
     6601 ccgcgggctg gcgacggtgg acgatcaggg cgatccccgc gccgagaacc tgcccgacga
     6661 tgagctcggc cggcgaggag aagaacaggc ccagcacgat cgggatctcg ctgaaagaga
     6721 aggtctgggt ttcacggcgc ggctggatgt ggatgacgtt gatctcggcg aatgcgaaca
     6781 tcgccgcgag cacccaccac ggcacgtgaa ggtgggatga gtacgcgccg agaccggact
     6841 cccctgcgag ccacaacgcc gcgccgatgg cggcgaagag cgccgtgacc gcccagattc
     6901 cggacttcct gccgggacgc cgcatcacgg gatcactccc atgccgcact cgaccaggtc
     6961 gtaccggacc acgtcgtgcc ggaccacgtc gtgccggacc acgtcgtacc ggaccacgtc
     7021 gtacccgacc aggtcgtacc gctccacgtc gtacccgacc aggtcgtacc gctccacgtc
     7081 gtgccgctcc acgtcgtgcc gctccaggtc gtaccgctcc aggtcgtacc ggcccagccc
     7141 atgccggacc aggtctgtac gcacaggcag ttgccgctcc aggtcgtgcc cagccactcg
     7201 ccacccgacc aggtcgtgcc gttccacgca cccgccgacc aggtggtgcc gtcccaggtc
     7261 ttgccgaaga tgtcctgctc gccctcgagc acgttgccgt tctcgtccac cacgtgcgcg
     7321 gagccgcgtg cctgctcgag cgagcccgtg cccgccgaca gggtccacgt ctgcgtgacg
     7381 ttcggtgtcg gcgcggcggc cgcgctcttg aggttgagga gccccgcgcc ctgcgcggtg
     7441 gccggcgcca gcggaaccga ggtcgcggtc gaggtcagca aagccttgag ctggtcgggc
     7501 gtgatcgaag gccgctgctg gatcagcaag gccgccgcgc ccgagaccac cgccgcggcc
     7561 tgcgaggtgc cgctgccgcg gaagaagcga gcgctggccc ggccgcccgg gaattgctgg
     7621 tcgatgtagg tgcccggtgc gcgaaggctg acgagcgact tgcccggcgc gacgagatcg
     7681 gggttgcgca caccgtcgcc gcggctcgac catgcgggaa tcgtgtcgtc ctcgacctcg
     7741 taggtgccct tggagtcgtc agcgccgacc gcgatcacga acgggttgcg cgccggattc
     7801 gccaggccga ccgtgttgcg tccgtcgttg ccggccgcca cgaccacggc gatcccgcgc
     7861 cgccacgcaa cctcgacggc gtaggacagc gggtccaggc ggcgatcctg cgtcgagtcg
     7921 gtgccgaacg ccaggttcat cacgcggatg ttcagaccat gcgcgttgcg gttctgcacg
     7981 acccaatcga tcgcggcgag gacctgggac acgtcggtcg cgccgtgcgc gttggcgacc
     8041 ttgatgttga cgatgcgcgc ctcgggcgcg acaccggtga aggtgtcgtg cccggtcatc
     8101 gcctggagcg atgtcgccgc gtcgtcgcgg ccgccgatga tgccggccag atgcgtaccg
     8161 tgaccgtagg tgtccatgaa gcgaaggttc gaggcctggg actcgaacga cagatcggga
     8221 ccgttgacga tcttgcccga ataggtgagc ccgttgaccg gcagcacgcc cgagtcgatc
     8281 atcgcgatgt ccacgccgcg gccggtgatg cccgacttcc acaggtcgtg cgagcggatc
     8341 gtcttggcga ggttgaagct ggatcccggg tcggtcgccc cgtcgaatcc gtcgaccgaa
     8401 tgcatcagcc tcaacgacgc gtcgggcgtg accgcaacga cgccgggaat ggctcgcgcg
     8461 cgatcgaccg aggatgccgg gatcgtggcc gcgaagccgt cgatgagggg cagcctggtg
     8521 ccgatctcgc cgcccattcg atgaagcgtg cgcgcgacat ggacgtctgc gccgggctcg
     8581 gcgcggacca ccacgccgat ggctgcggcg gccggcgcga cgaccgcggc ggtggccgcg
     8641 accaacacga cgaccgacag gagcgaacga gcgatccgat ggacgggcga gatcaagttg
     8701 tgcgctcccc taagcaggac tgggttccca gaagaaagat gaccacttat tttgcggtct
     8761 gcggacagac cccgtcaatg ctgcttacca ggacttttgc tcctcctaag gtgatcggcg
     8821 ggcccccggg aaatctgaag cttctctcgg ccacgtctgg gcccgggtcc ggcttgtttc
     8881 gaacggtccc ggtgccgccg aaaaggagca agcaatatgg atggttcggt acggatatcg
     8941 agatgatccg cgaacgggcg cggcgccaca tcgaacaagg cccgatccac cggagaccgc
     9001 aagcgcgtca tcgacgtgct caacgggatc ctggcgacca gaccgtgggc accctgcgct
     9061 acaggcggca ccctgcgcta caagcggcac cctgcgctac aggcggcacc cttcaccgcc
     9121 cagggcatcc atgccggccc ggtcgcgacg agttcttcga gcacgccaac gacctgccga
     9181 gcctgatggc cgggatcgag cagcgctgac cttgcccatg ctcacggccc ggggcgatga
     9241 tccccgggcc gtgttcgttt ctcgagggga gcgcttgcat gaacgtcgcc gtgctgggaa
     9301 ccggaagcgt cggccgcacc gttgccggca cgatcggatc gctgggccac gaggtccgga
     9361 tcggaacgcg cgacgtggag gcgctgatgg cacgcaccga cgacgccccg acgtttgcgg
     9421 aatggatcac caccgtgacc agcgtgcagg ccatgacgtt caaagacgcg gccacgttcg
     9481 gcgagatcgt gttcaacgcg acctcgggca cggcgtcgct ggaggcgctg cgcgccgcgg
     9541 gcgccgccaa cctggccggc aaggtgatcg tggacatcgc gaacccgctg gacttctcga
     9601 agggctttcc gccgacgctg tcggtctgca acgaaacctc gctggccgag cagatccagg
     9661 ctgcgttccc cgacgcgcgc gtggtcaaga cgctcaacac cgtcaacgcg tcggtcatgg
     9721 tcgatccggt ctcggtcggc ggcggcgagc acgacatgtt catggccggc gacgacgacg
     9781 atgccaagcg cacggtcgag tcgatcctgc gcgactggtt cgggtggacg cgcgtcaccg
     9841 acctcggaga catcaccaac gcacgcggca tggagatgta cctgccgctg tgggtgcgcc
     9901 tgtacggcgc agccggaacc ggcgccttca acatcgcgat cgtgagataa gaagaggggc
     9961 ggccgcacgg ccacccctct tcggtgcgtt cttggtcaac ttcgggtcct ctatgtgcct
    10021 cgcgttgacc aagaacgatt cgctacagcg actgcgacac cggcggagcc gggagcggct
    10081 gtcccccggg accgcccgcg aaggtcatcc acggcgtcga ggtcgccgac tcgatgccgg
    10141 gcttggacca gtcgcagacg ccgcccgcga acacggcctg caagcgtgac cactgcccgt
    10201 cggtgaacga cactcgatag tcgcggcggt cgagcggctt gagcgcgcac ttggcgatgt
    10261 cgtgcgtcgc cggcccgccc gcggcgatgc gcggcgcgcc gtagtagggc acgaccgtgc
    10321 ggcacagcgt cgcgtcgctc acgcgctgcc ccgcgaccca gcacgagtcc accgcgtccg
    10381 ccggcttgtt ccggcggatc ttctcggcgc gaggcagcgc gctggtgtcg gcttcgacgg
    10441 cggtcagcca gcggtccagc agcaggaacg actcggtcgc ccagaccggg tcgccgacca
    10501 gcggaacgtg gctggaccaa atcacgtggt tgtccgcgtg accgttggcc ttcttgagcc
    10561 gctcgcgcat cgagtaggtg tggaagtcgg tatggatctc ttcgttgctg gatccgcgaa
    10621 ggtcgatgat cgcgacctcg tcgagctgcg cgccgtcgtt gatctggccg ctgcgctcga
    10681 gcgtgttgat cgagccggga tcggcggtgc ggcgcccggg cgcgaagttg aagtcgatgt
    10741 ccaccgcgcc gatcttttcg ttcaggtcca cgaactgctc ggcggtgatc aatccagagt
    10801 tcaccgcgtg ccatccgtac tgcacgccga cgttgtcgta gggcagcggc gcgaatccgt
    10861 cctgcgcgcg ggttcccagg accggcttgt agtagtcggc caggctgcct cggcaaccac
    10921 ccgggttgct ctgagggtgg tactcctgct cgggcgggag gccgcatccg cccttggggt
    10981 cggcgaccgg agcgaacagc gcttcccacg cggcgcagct ggtcgggctc tggtggccgt
    11041 tgacgaaggc ctgctgctgc gcggccgccc ataggtgcgg cgaggtgcgc acgtagtagt
    11101 tgagcagcag atggcagtct acgacctcca tgccggtgga ccagttgtcc tcgtagctgc
    11161 agttcggctg gatgccgtcg atcaggcccg ggtagccgtt ggcgaccatg tgctggccga
    11221 tcgagccgcc cgagcagccg ctgccgatca cgaagcggat ctcgccgtag cgctcgatga
    11281 ctcgctcgcg cagcatcatc gcggcctcgg cgctcacgac ggtgttgcag ttgttgccga
    11341 ggttgttcat cgaggagttc acgaccatga agccgcgcga gagcgcgcgg tcgtcgagca
    11401 cgttcgccgg cgaggactgc gaatggatcg tgccgcacga cgcgccgaat acgtaatgga
    11461 tcttgccgtt ccagccttcc tggcgcgccc acggctccca gccgcgcgcc gggtcgaaca
    11521 gcacggcgat gtcgtagatg ccgcggtccg cggtcccgcg ctcgcgccgg acgatgtagg
    11581 gaacgacttc gccctgatcg gtcgtggtgg tcgcgacgtc ggccggcggg ttgttcgggt
    11641 cgtaggcaag gaagccggtg cgggccgggt tcgtggagcg atagtggtac gtgtggacgg
    11701 tcggggcgtt gcactgcgcg tcctgcgcga caccgagacc gttgctttgc gtggtgcaca
    11761 gccacggctg gatctgcgtg cccgcgaaga ccgggccgcc gatcgcatgg ttggtgatcg
    11821 ggatcgtcgc gccggccagg ccggcgcgcg cgttgaggtt gttcacaccg agcgcgagcc
    11881 cctcgactcg gccgaggaac cggccgtcgg cgcggaccgc gaacgatgac gtcacgtcgc
    11941 gaccgtccac gtccacggtg acgcccgccg ggtcgacgcc gccggcgagc acgatttcga
    12001 cgagcgcgtc cccacccgag atcagatcgg ccctgttgga cagaacccgg atggacacgt
    12061 cgtccgcggc ttgcgatacg acggggagaa cgatgagcag cgaggtgacg acggccagaa
    12121 tcaggctccg tctcatgggg gtgcctcctc ccgataccgg gcccggcctc ggtgccgggc
    12181 ccctgcgggt tccctttcgc cgcttccgcg acgattcctc ccccacggtc ggaattaggc
    12241 ccgcggcagg tcgaggcgct tcaggagcag cgcgttgacc gcgacgagca cgctcgagcc
    12301 ggacatcgac aaggccgcga tctcggggcg cagcatcagc ccgaacgcgg ggacgaacac
    12361 gccggccgcg atgggaagcg cgagcgcgtt gtagccgatc gcccagccca ggttctgacg
    12421 catcttgcgc agcgtgccgc gcccgatggt cagagcggtt gcgacgtcga gcgggtcgga
    12481 gcgcatcagc actacgtcgg cggtctcgat cgcgacgtcg gttccggcgc cgatcgcgat
    12541 gccgacctcg gcctgcgcca gcgcgggggc gtcgttgatg ccgtcgccga ccatcgccac
    12601 gcgcttgcct tcccgctgca gcgccacgat ctgcgcggcc ttgtcgcccg ggagcacgtc
    12661 ggcgatcacc cgatcgatgc cgagctcggc ggcgatgcgc gtcgcggtgg cttggcggtc
    12721 gccggtgagc atcacgacct cgatgcccgc gtcccgcagc gcccgcacgg cggcgcgagc
    12781 agtcgggcgc ggcgcgtcgg cgatcgcgat gctgccggag taggtcccgt ccaccgcgac
    12841 gccgacgacg gtgcggcccg cttcggtctg atcgcttcct tcgatcgccg cggccccgac
    12901 ctgtttcatc agccggtcgt tgccgaccgc gacccgccgg ccgtccaccg tcgcgacggc
    12961 accgtggcct ggcacggcct cgaagcgggc cgcgcgtacg gcggggatgc cgtggccgat
    13021 ggcgtgccgc acgatcgcct cggccagcgg atgttcggat tcggcttcga cggccgcgac
    13081 cagacgcagc agatcatttt cgtcgatgcc gcgtggctcg accgcgacca cgaccggctt
    13141 tccttcggtc agcgttccgg tcttgtcgag cacgaccgtg tcgatgcgtg cggacgcctc
    13201 gatggccatc gcgttcttga acaggatgcc gcggccggcg cccaggcccg cgccgaccat
    13261 gatcgccgtg ggcgtggcca gaccgagcgc gtcggggcag gtgatgacca cgacggtgat
    13321 cgcgaacagc atcgcctcgg tgaccggcgc gccgacgccc caccaggtca ggaacgtggc
    13381 cgcgccaccg atgagcgcga ccagcaccag ccagaaggcg gcgcggtcgg ccagacgctg
    13441 gccgggggcc ttggattcct gcgcggcgcg caccagctcg acgatctgcg cgagcgcggt
    13501 gtcggcgccg accttggtgg cgcgcacgcg cagcgtgccg tttcgattga tcgttgcgcc
    13561 gatgacctcg tcgccggagc gcttgccgac cggcatcgcc tcgccggtca ccatcgactc
    13621 gtccacgctc gactcgccct cttcgaccac gccgtccacc ggcagcttcg aacccggacg
    13681 caccagcagc agatcgcctg cggcaacctc ggcggtcggg atgtcgacga tcagaccgtc
    13741 gcggatgacg gtcgcggccg gcggcgacag gtccagcagc cggcggatcg cgtcgttggc
    13801 accgccgcgt gcgcgcatct cgaaccagtg gccgagcagc acgaacgcgg ccagcacggt
    13861 cgcggcctcg aaaaagacct ctcccccgcc ggtcagcgtc acgccgaccg agtagatcca
    13921 gcccgcgccg atcgcgaccg cgaccagcac catcatgtcg agcgtgcgcg cccgcagagc
    13981 ggcggccgcg ccggagaaga agatccagcc cgagtaaaag accaccggga tgctcagcag
    14041 catctgggcc cactcgtcgc tcatgcccag cggagccgcc gcgctgaagc cgatcacgtc
    14101 gcgcccgatg cgcgagaaca acgtgatcgg gatggccagc acggccgcga ccaggaagcg
    14161 gttgcgcatg tcgcgggcca tctcggccat cgacatcccg gcgtggcccg cgtgaccgcc
    14221 ttgcccgccg tgcccggccg gagcctcgtg gccgccgtgg cccgccggag ctgcgcggcc
    14281 gccgtggggg gccgaaggat cgtaggcgcc ggcggcgtcg ccgtcgtgct cgccggacgc
    14341 gtgctgctcg tgggggacct cgtggcccgt cgtcgccttc atcgcttccc ctttaccctc
    14401 acaatacccc cagggggtat acccagggcc gcactatacc cccatggggt acatggatgt
    14461 caaggccgga cgacgccttc ctcccttgtc cggcacgagg ctccggcagc gatgcttgct
    14521 tgccgggcgg tcctttcgtg aatttcaagg gcctttttcc gattcttggg caggcacgaa
    14581 ccggcacatt gtcgaattgc cgttccaggc gccggcaccg gccggagcaa cgagttggag
    14641 gatgcaatgg ctcagcgccg cgccacggtc cgagaccgcg ctcttcgcat cgcctcggcc
    14701 cttgccgtcg cggccgcgct cttccccgga accgctcccg cgcgtcaggc cacgcatgcc
    14761 cgatacgaca tcgaccggtt ccgcgaggcg gccgcagcgg ccgtgacacg ggtcggcgcg
    14821 ccacccgccg ccgccgcgag ctacttcgac gaccacacct tcagcttcgc gcacaccggc
    14881 gaggatccgg tgacgctctc acagccggac ggccaccggt tccgcgtgcg gcagcgcccc
    14941 gcgagcgacg gcggcggcct ggagaccgtg gagggggagc tcgtcgtccg cggcgatgac
    15001 gagatctggc gctacgcccg ctggggcgag acgaccaggc tcgtcgcgac cgacgccgtc
    15061 gtcgggcgcg atccggctcc gccggtctct cacgccacgc ggtattcgcc ggcgatgaag
    15121 aaagcgctca ccgaagacgc ggtccgtcgc gaagagatgc gccgctacac cgcgacccgc
    15181 gcgcgggccg tcgccgacgc cgccgcagcc gcgggcgagc cggtcgttct gcgaatcccc
    15241 gtcctgctgt tcaagatcaa caaccgcgac ttccttcccg gcacgacgcc ggcctcggtg
    15301 gaggcgctca tcgacgggca cggcacgagc atgcacggca cgctcgccga gctctacgag
    15361 gagcagagcc acgggcgcct gatcctcgag ttcgacgtct tcggtcccta cgacgtcagc
    15421 gtctcgaaca atccgcagaa tgactgctgg tacggaacgc tcggcggccc ggtcttcggc
    15481 gacgtgctcg gcatcggcgg cctgggcgcg aaaggcatgg cgcaggaggt cgtgccgcag
    15541 gcggatcccg acgtggactt ctcgcgctac gacaacgacg gtgacggcta cgtggacttc
    15601 acggcgatca tgcactcggg aaccgcctac gaggtcaccg gctcctggtg cgatgtgtgg
    15661 tcgcactact ggagcggatt cggccccccg gccgggcttc cgccggaccc gacgctcacg
    15721 accgacggcg tggtggtggg atccgtcctg acggtgcccg aggtcgcacc gttcacgggc
    15781 gtgttcggcc acgagatcat gcacgcgctc ggcgagcccg actactacgg cacccaaggc
    15841 acgtcgggga cgggcgactg ggatttgggc gcgggcggcg gatacctcgg ccagccttcg
    15901 caatcgaatc ccgctcacct caacccggtc gtcaagatca acatggggtg ggtcgagccg
    15961 gagatcgtgg atgccacgac ctatgacgtc gagatccgcg cccgcgcgac ccatcccgac
    16021 gccgtcgcca tccccttgac cgtcgaaccg gccggcacca gagccgatca actctgcggg
    16081 gctcaggcga tcgggggctt ccccgaacag aaccgccgat tccggcagcc caacggcgac
    16141 tgcatcgtcg aggggctgct gatcgagcac gtttcgcaat cggccggccg ctatgagaca
    16201 gccccgggcg gcgactgccg tttcgttccc gcgcacttcg accggcaggt gtacgcgtcg
    16261 gggctcgcgg tctggcactt cgacttccgg tcgtacacgc agctcggcaa caacaatctc
    16321 ggacggccga tgctctggct ggtcgagttc gaccgccgcg acgacacgca ggagattcgc
    16381 tcgggggtca cgcgaggaga gccgacagat ctgtactggg gtgacccggt gggcatcagc
    16441 acggcgaccg gaagccgcgt cggcgccatc gcgccgccac cgccgggcag cccgtacacc
    16501 gcttccgcac cgttgcccgg cgccgcgcag caaacgcccg actggacggt tccccccgag
    16561 gccgactcgc agacgatgac cctgacgctg acgtgggaca cgcagaatct cgacgactgg
    16621 gacctccatc tctacaagga ggtggagtcg gacggagaga cgtactggga agaggtcaac
    16681 gccgcggccg gccccggcgc cagaggaccg gaggtcgtgg atcatccggt cgccgcagga
    16741 gaaaagtact tcgcgttggc gagaaattgg gcatccgtct cgccggaggc acgcatcgag
    16801 gtcgtctacg gagcccgggg atcgggttcc aaggcgagca ccctgaccaa caacggagtc
    16861 gggctcggct ggcagatcac cggcatgagc tcaccgtcgt cgagcgggac cgcgcatgcg
    16921 gcgcagcggc cgggcgagcc gatcaccctc gacatcgtcc ggcacgacgg aacgacggtt
    16981 gacgtctcac cggacttcct cacgccggca ggaggcgccg tgccggtcat cgccggcaag
    17041 ccgatcgaga tgtccacccg cctctacaac cacggcggca aggccgtgga ccaggcgacg
    17101 atctccctgt tcggcgtcga tccgtccacg cctggggcga cgccgctgtc gaccgtgacc
    17161 cggagcctgt cggcgttcga ccgccaggac gtcaccttta ccttcaaagc ggccgccgga
    17221 acgcgtacgt actacgtgcg cacggtcgcg gccggcgacg tccacccatc caacgacgtc
    17281 gtcgcgaccg agatctcggc cgacccggcc tcggccaacg tgctcgtcgt ggacgccgac
    17341 cgtggcttca ccttcgaaga gcccgtcgag gccgtcctgc agggcctcgg cgtgccgtat
    17401 cacgtcgtgc gcggcatccc gaccctgacg acgctgcgcg cctacccgct ggtcgtgtgg
    17461 ctcacaacga gcgtctccgg acccgacggc atctttcact actcgtcgca cgaagccctc
    17521 tgggattacc tcgctggcgg tggccgcgtc tggatgcaat ccacgcgagc cctcgccacc
    17581 tcgtggatct tcccggaatt cttcggtatc ggctcgagtt ggttcgcacc cgactcccag
    17641 ggacgcgcgc tgccgaagag cgccaccact ctgcccgcta tcgatctcgg tacgct
//
LOCUS       DATLFO010000292         5942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_96848, whole genome shotgun sequence.
ACCESSION   DATLFO010000292 DATLFO010000000
VERSION     DATLFO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96848"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VM841_11795"
     CDS             complement(<1..276)
                     /locus_tag="VM841_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90904.1"
                     /translation="MTLRRQLVIVFAVFAAALVTATIVPLGVASSRANLASFIEQRVA
                     HTIRVAESVGRAPDTLPDRYPATSGDAGWLLDGVTAGGRIAVGQRVGR"
     gene            complement(273..962)
                     /locus_tag="VM841_11800"
     CDS             complement(273..962)
                     /locus_tag="VM841_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL90905.1"
                     /translation="MGFAGRVLVVEDEKSIVGPVRRALKREGYEVEVSDNGTDALRVV
                     AEWDPTVVLLDLMLPDMDGHEVARRIRAASHVPIIMVTARGAESERVAGLELGADDYV
                     VKPFSLPELLARLRAVGRRVSAAPPPGNELSFKDIRLDLGAYRAYKGGSELHLSKKEF
                     ELLRMVMAKNGNLARRDDLITAIWHIAPNESGKTLDVHMSWLRKKIGDDPKRPRYIQT
                     VRGVGFRLVDS"
     gene            complement(1036..4407)
                     /locus_tag="VM841_11805"
     CDS             complement(1036..4407)
                     /locus_tag="VM841_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90906.1"
                     /translation="MNIKRILTVLALLAVIAPAGAASAPPPQGRALVADAGDSAFLAE
                     GMAHGLSGMAWGGRAPYRFSWSGPGGTARFTDAAAASTAFDTRGLKSGDYMLSLSVRD
                     AAGRSARDSVKIRVFETKPRTILEESDTPPFGLPDEMILGHGADSPDGQTREYPFSLP
                     AGLLRLDLNLEWDTYVGALGVYGVNDYDLYVEGPDASYADIVSGATGSHPEKISIDKP
                     KAGRYKAIVSAFLSVADEFRLTVGVVDQPGTSPLPEIQIPPVIRFATGAPQTLSAKAP
                     KGGAVSWDLNFDGVFETAGASVAASFPRGTHLATVKVVKNGFEIRRTVGVTVLRAGEI
                     AQNTTPFVIVGIGDSGINPYHDEFSAASYPDPEVLRITEGFSKHPSAYIPGYPAGTPA
                     LPVTLGKGYLPEEDKNLWTREAIQFQKLYWIPGTKIVGAIDWTDSSGSNANADTRPIL
                     DDDGHGTESSSVSVGNRYGNCPACLLVFAEGLQGDDYLVTQSWIDFVSMSVGSLGNVG
                     FGIFDAEPSRVAAERGQTVLFAAGNGFGNAFDAPMSTYTSATAGPDWHVIVGAARTDN
                     RRPINGDAAPVDISSWGDGTIPAACKEGTVSMCNFGGTSAATPLTAGVFGQVLREVRR
                     AIGDTGAGQRPGQVVAQGVAISKSPYLRDGKLTRAELWDIVLHTAEPFGEEGVPVPPY
                     THTWPGPRSTDFLMGGYGLATPESAEHAIDVALGRAPIPHRPVEDTFFAYDKQVRTAM
                     WGEWDSDEDSVANPGDLAGAVSVPQQAIDPDAISSLTGGAPAELPEGASIDYYLHHRG
                     GCPELAPPADPAGLFPSAGYTFMDRENSDGDDEPCPNTRLTTVAAYFRPVGIWAAAAP
                     VGRFLPAGSGVDASVFLTMDHPMALVASSQLLAGKRIVGSGASQVTPVVDVMAARCRA
                     GLPGCWTEVKIRFVTDRPIGAHERLTFQVGLRSSEGTYFGYEGDHASKVSITPAVGAS
                     ASDLVAVIDSPADAASLTSSTVTLRGTATFGDVESESLRKVEVSIDDPLFGAPIAATL
                     GEPREDGTVPWSAAVSLPGGAHTVFARALQDRRASERDSVRLTVGAQVLGIGKAPLPA
                     TGIGGPLAGILPVLLACAGALALRRRDNGGRISAP"
     gene            4890..>5942
                     /locus_tag="VM841_11810"
     CDS             4890..>5942
                     /locus_tag="VM841_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL90907.1"
                     /translation="MNIAFGVTWNDAKKGPKRAAAMALAAAMIATLIPRSDAAATAQS
                     LVSVIVRALPGAEQAAEDAVTSSGGRVGRSIGIIDGFVADVPAGELARLSRIGGVASV
                     SPNVPVTLTHAVDGYDASTDGHSMWTIATVTKATTMYSEGFTGRGVDVAVIDSGVVPV
                     NGLTASGKVLNGPDLSFESRSDSIRYLDTYGHGTHMAGIIAGRDNAVTYRGRWWTNHD
                     DFTGMAPDARIVNVKVANAQGTTDVSQILAAIDWVVQNRARNGLNIRVLNLSFGTDSV
                     QSWVLDPLSYAVDVAWRKGVFVVAAAGNNGNALGSLSNPAYNPRVMAVGAADANGTFS
                     IHDDTIPPWSSRGDGIR"
BASE COUNT          953 a         2032 c         2045 g          912 t
ORIGIN      
        1 gcgcccgaca cgctgcccga ccgctatcct gccaccagcg gtgacgccgt cgagcagcca
       61 tccggcgtca ccgctggtgg caggatagcg gtcgggcagc gtgtcgggcg cacggccgac
      121 ggactcggcg acacgaatcg tgtgcgcgac gcgttgctcg atgaaggatg cgagattggc
      181 gcgcgaggaa gcgactccga gcggcacgat cgtcgccgtc acgagcgccg cggcgaagac
      241 cgcgaagacg atgacgagct gccggcggag cgtcatgagt caaccagacg gaaccccacg
      301 ccgcgaacgg tctgaatgta tcgaggccgc ttgggatcgt ctccgatctt ctttcgcagc
      361 caggacatgt gcacgtcgag cgtcttgccg ctttcgttgg gggcgatgtg ccagatcgcc
      421 gtgatcaggt cgtcccggcg agcaaggttg ccgtttttgg ccatcaccat gcgcagcagc
      481 tcgaactcct tcttggacag atgcagctcg ctgccgcctt tgtacgcgcg atacgcgcct
      541 aggtccagac ggatgtcctt gaaggaaagc tcgttgcccg gcggcggggc cgcgctgacc
      601 cggcggccga cggcacgaag gcgcgcgagc agctcgggaa ggctgaacgg cttgacgacg
      661 tagtcgtcgg cgccgagctc cagtccggcc acgcgctcgg actcggcccc gcgcgcggtg
      721 accataatga tcggcacgtg ggaggcggcg cggattcgtc gcgcgacctc atggccgtcc
      781 atgtcgggca gcatcaaatc gagcagcacg acggtcgggt cccactcggc gacgacgcgg
      841 agcgcgtcgg tgccgttgtc ggacacctcg acctcgtatc cctcgcgttt gagggcacgc
      901 cggacgggcc ccacaatcga cttctcgtcc tcgacgacca gcacccggcc cgcgaaaccc
      961 aaattctctc ccccctcgcc gctgttgtca gacggaattt agaatgcggc gagttaagca
     1021 gaagttatgc ttcagttaag gggcggagat tcgtcctccg ttgtcacgcc ggcgcaacgc
     1081 cagcgcgccc gcgcaagcaa gcagcaccgg caggataccc gcgagcggac cgccgatgcc
     1141 cgtggcgggc agcggggcct tgccgatccc gagcacctgc gcacccacgg tcaagcgcac
     1201 cgagtcccgc tcgcttgccc tgcgatcctg aagagcccgg gcgaagacgg tatgcgcccc
     1261 gcccggcaac gacaccgcgg ccgaccacgg cacggtgccg tcctcgcggg gctcgcccag
     1321 cgtcgcggcg atcggcgcgc cgaacagcgg gtcgtcgatg gagacctcga ccttgcgcag
     1381 cgactcggac tccacgtccc cgaaggtcgc ggtgccgcga agggtcacgg tcgaggacgt
     1441 gagcgaagcg gcgtcggccg gcgagtcgat cacggccacc agatcgctcg cgctcgcccc
     1501 gaccgcaggc gtgatcgaga ccttcgaggc gtgatcgccc tcgtatccga agtaggtccc
     1561 ctcgctgctc cgcagaccga cctggaacgt gaggcgctcg tgcgcgccga tcggccggtc
     1621 ggtcacgaac cggatcttga cctcggtcca gcagccggga aggccggcgc ggcagcgggc
     1681 cgccattacg tccacgaccg gcgtgacctg cgacgcgccc gagcccacga tgcgctttcc
     1741 ggccagcagc tggctcgatg ccaccagcgc catcgggtga tccatcgtca ggaacaccga
     1801 ggcgtccacg cccgagcccg cgggcaggaa ccgcccgacc ggcgccgccg ccgcccagat
     1861 gccgaccggg cggaagtagg cggcgacggt ggtgagccgc gtgttcgggc acggctcgtc
     1921 gtcgccgtcg gagttctcgc ggtccatgaa cgtgtagccg gcgctcggga acagccccgc
     1981 aggatcggcc ggcggcgcca gctcgggaca gccgccacgg tggtgcaggt agtagtcgat
     2041 cgatgcgcct tcggggagct ccgcgggcgc gccgcccgtc agcgacgaga tcgcgtccgg
     2101 gtcgatcgcc tgctgcggca cactcaccgc gccggcgagg tcgcccgggt ttgcgactga
     2161 atcctcgtcg gagtcccatt cgccccacat cgcggtgcga acctgcttgt cgtaggcgaa
     2221 gaacgtgtcc tcgaccggac gatgcgggat cggggcgcgg ccgagcgcaa catcgatcgc
     2281 gtgctcggcg ctctcgggcg tcgccaagcc gtagccaccc atcaggaagt cggtcgagcg
     2341 cgggcccggc cacgtgtggg tgtacggagg caccggcaca ccttcttcgc cgaaaggctc
     2401 tgcggtgtgc agcacgatgt cccacagctc ggcgcgggtc agcttgccgt cgcgcagata
     2461 ggggctcttg gagatcgcga cgccctgcgc cacgacctgg ccgggccgct gcccggctcc
     2521 ggtgtcgccg atcgcgcggc ggacctctcg cagcacctgg ccgaacacgc cggcggtcaa
     2581 cggcgtggcc gccgaggttc cgccgaagtt gcacatgctc acggtgcctt ccttgcacgc
     2641 cgcggggatc gtgccgtcac cccacgacga gatgtccacc ggggcggcgt cgccgttgat
     2701 cgggcggcgg ttatccgttc gtgccgcgcc cacgatgacg tgccaatcgg gaccggccgt
     2761 cgccgaggtg taggtgctca tcggcgcgtc gaaggcgttg ccgaagccgt tcccggcggc
     2821 gaacagcacg gtctggccgc gctcggccgc cacgcgggac ggttcggcgt cgaagatgcc
     2881 gaatccgacg ttgccgaggc tgcccacgct catcgacacg aagtcgatcc aggactgcgt
     2941 gaccagatag tcgtcgccct gaagcccttc cgcgaacacg agcagacatg cgggacagtt
     3001 gccgtagcgg tttccgaccg agacgctcga ggactcggtg ccgtgaccgt cgtcgtcgag
     3061 gatcggccgc gtgtcggcgt tggcgttgct gcccgacgag tcggtccagt cgatcgcgcc
     3121 gacgatcttc gtgccgggga tccagtagag cttctggaac tggattgcct cgcgggtcca
     3181 aaggttcttg tcttcttcgg gcaggtagcc cttgccgagc gtcaccggca gcgcgggcgt
     3241 gccggccggg tagccgggaa tgtacgcgct cggatgcttc gagaagcctt cggtgatccg
     3301 cagcacctcc ggatcggggt aggacgccgc cgagaactcg tcgtggtacg ggttgatgcc
     3361 cgagtcgccg atgccgacga tgacgaacgg ggtggtgttt tgcgcgatct cgccggcccg
     3421 caggacggtt acgccgacgg tgcgccggat ctcgaagccg ttcttgacga ccttcaccgt
     3481 cgcgaggtgc gtgccgcgcg ggaacgaggc cgcgacgctc gcgccggcgg tttcgaacac
     3541 gccgtcgaag ttcaggtccc aggacaccgc accgcccttg ggtgctttgg ccgacagggt
     3601 ctgcggtgcg ccggtcgcga agcgaatcac cggcgggatc tgaatctcgg gcagcgggct
     3661 ggtgccgggc tggtccacca cgcccacggt caggcggaac tcgtcggcga cggacaggaa
     3721 tgccgacacg atcgccttgt accggccggc cttcggcttg tcgatcgaga tcttctcggg
     3781 atgggacccg gttgcgccgc tgacgatgtc ggcgtacgag gcgtcgggcc cttcgacgta
     3841 gaggtcgtag tcgttgaccc cgtacacgcc gagcgctccc acatacgtgt cccattcgag
     3901 gttcaggtcc agacgcagca ggccggcggg caggctaaag ggatactccc gcgtctgccc
     3961 gtccggcgag tcggctccgt ggccgagaat catctcgtcc ggcaggccga acggcggcgt
     4021 gtcggactcc tcgaggatgg tgcggggctt cgtttcgaac acgcggatct tcacggagtc
     4081 gcgcgcgctg cggccggcgg cgtcgcgcac cgacagcgac agcatgtagt cgccggactt
     4141 caggccgcgc gtgtcgaatg cagtcgaggc cgctgcggca tcggtgaacc gggccgtgcc
     4201 gccgggaccg ctccacgaga agcggtaggg cgcgcgtccg ccccacgcca tgccgctgag
     4261 cccgtgcgcc atcccttccg cgagaaaggc ggagtcgccc gcgtcggcga cgagcgcacg
     4321 accctgcggg ggcggcgcgg atgcggcgcc cgcgggggcg atgacggcaa gcagtgcgag
     4381 cacggtgagg atgcgtttga tgttcatgcg cgaatctcca acccggtgac ggcgcccaac
     4441 ccctgggcac cgcatttcac gtcccgttca gtatgaaggg tatggcctcg tcccgcgaca
     4501 gggcattcgc ccgcgttgcg gcacgaccac gcgatgcggc acgaatgcgc ttcggcctcg
     4561 ttacccgacc aatgccgcgg gcatccggga cccacgcacg ggaagcgata caacccgaca
     4621 tgtcggttcc ttgcccttcg tcccgtggtc ccgaaaccca tgccccggtt gcggtagcga
     4681 accccctctc accgggccgg tccggccggt gaccgcacgc gcggatgctg cggaaatgca
     4741 atccgccctg tgcgaaggcc ggcggcggac aggcatccgc atagtcattc ggcacgagaa
     4801 gttcccggcg gatccggact ttggtccctc gtgacgcgac agccggctgc ccgatacttc
     4861 aaggaccggc gagctcggaa ggaaccgcta tgaacattgc gttcggcgtc acctggaacg
     4921 acgcgaagaa gggaccgaag cgtgccgcgg ccatggcgct cgcagcggcc atgatcgcta
     4981 cgctgatacc ccggagcgat gcggccgcca cggcgcaatc gctggtatcg gtgatcgtgc
     5041 gagcgcttcc cggcgccgag caggcggccg aggatgccgt cacgtcctcc ggcggacgcg
     5101 tgggccgctc gatcgggatc atcgacggat tcgtggccga cgtgccggcg ggcgagctgg
     5161 ctcgcctgtc tcgcatcggc ggggtcgcct ccgtctcacc caacgtcccg gtcacgctca
     5221 cccacgccgt agacggctac gacgcatcca cggacgggca ttcgatgtgg acgatcgcca
     5281 cggtgacgaa ggcgacgacg atgtactccg agggcttcac cggacgaggc gtcgacgtcg
     5341 ccgtcatcga ctcgggcgtg gtcccggtca acggcctcac ggcaagcggc aaggtgctca
     5401 acgggcccga cctgtccttc gaatcgcggt cggactcgat ccgctatctg gatacctacg
     5461 gccacggcac gcacatggcc ggcatcatcg ccggacgcga caacgccgtg acgtaccgag
     5521 gaaggtggtg gaccaaccac gacgacttca ccggcatggc gcccgatgcg cgcatcgtca
     5581 acgtcaaagt cgccaacgcg cagggcacaa ccgacgtgtc gcagatcttg gccgcgatcg
     5641 actgggtggt gcaaaaccgc gccaggaacg gtctgaatat ccgcgtgctg aatctctcct
     5701 tcggcacgga ctcggtgcaa agctgggtgc tggatccgct gtcctacgcg gtggacgtcg
     5761 cgtggcgaaa gggcgtgttc gtggtcgcgg ccgccggcaa caacggcaac gcgctcggct
     5821 cgctgtcgaa ccccgcatac aaccctcgcg tcatggccgt cggtgccgcg gatgcgaacg
     5881 gaaccttctc gatccacgac gacaccattc ccccgtggtc gagccgcgga gacggcattc
     5941 gc
//
LOCUS       DATLFO010000293         4770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_96870, whole genome shotgun sequence.
ACCESSION   DATLFO010000293 DATLFO010000000
VERSION     DATLFO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96870"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VM841_11815"
     CDS             <1..219
                     /locus_tag="VM841_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90908.1"
                     /translation="GGGFTGGGEFPSTGPLLPGYENTPPVPVDKVTAAVEALGAGAPA
                     GVKALYGGMAFALAMLVAGRQVFRTLAR"
     gene            235..687
                     /locus_tag="VM841_11820"
     CDS             235..687
                     /locus_tag="VM841_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90909.1"
                     /translation="MDDRDDRERHQAAPEPEAGGRLLLGWILIVLGVVMVFAGWLGVS
                     GNPQVAVQLPYLISGGIGGLLAGIVGVGLLVSDDIRKDRARIGRLEAAVLEMREFAAA
                     QAEAMGLAGSRESREEGGSDDETTVRPYRAAGEQPAANGRIRAGARRR"
     gene            853..1500
                     /locus_tag="VM841_11825"
     CDS             853..1500
                     /locus_tag="VM841_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90910.1"
                     /translation="MRVRITKAVLTAALAAMAIIGTTPAGAVSAYHMNGQGTITMQGA
                     GLPSCLPVVLQGDCTHSDQTFSWGTLTVQGAGILQDAAVAGQYRCKSEGDVGGTAFVS
                     KIRGNTSNFELTFNFQCQNLSGVGPTFINGWFSNINGSAAAPYRSVAGHPHPTPVEKG
                     VFTFKGYFTKTNTHPYNINNGNGDAMTCVGGFAPSGFNGLKITTAAFAGACTAAE"
     gene            1581..4331
                     /locus_tag="VM841_11830"
     CDS             1581..4331
                     /locus_tag="VM841_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90911.1"
                     /translation="MTERKGRRLVRTAALTLALVFLAPAGLPVHAASDPITQAGALDT
                     SMIGARRHLDADPSARRVFEIGQPVDQGGMRVDAWDLDTLRPAGSVGIAEAQSGQGGM
                     LSTVDELRHRLYVLYPVKAADDTVIRGVLGPGISTGIYDEATRALFGVAAIDTLRMKV
                     IGSGTLALRVPPVPRDTSESNFVGIKAMSYFREGGRDLLYLASEVPTGQGNLVVSNHT
                     VAIHQIDAQRLIDPASTLTAVDWSMPLPQCALVMARGTPGVLFRSAREPVVALPCKQG
                     GAAGVAGQPAETPGIVRVRVTPGATQTDVSTFVTTFHAISGNLQYGVVAADPLRERIF
                     MQIDGSNTEAAIWVFDVPTATFIGIVALPTAFASGGTQALGVDPSLARLYAISTPSGE
                     ILRSTVLITSTDTTVVDQGLITTFRPQPAGSKSRPVVDPIRHRIVMSAVGGSIVLQDS
                     LPQRGGAGAAPDPDAGTVDAAEGAGTAVNFLGGGQAYGARIRWVGGVGGADRNLNTVL
                     DPPPSELSPQTGGITPPPPAPGTRDVHFARVLHTTLTNGEASAKATGAQRDHENTDAD
                     LGGMANYLRRWGGPDLADQRWPYPEVDCTDFGGAGDTRNKKSYGAEVACRQSDATVTA
                     LALDESLSQGGVMRIAQARARSVVARDPKRGVVSIVESEMLGVNLGGRAGIGRVLARA
                     ETWASGRPKSSNGVGAGGSFRRIFEDVWIVDDAGSRRTVCSDLCDPEEVRDSINRALG
                     LRARIDLPEPEPARMSGTPGGFEALIVRDPFERANEASVNEEADARRLEVPAMVVTVF
                     ADGRVPSRVVISLAGVSAESHYGIFLLAQGGTFRPPGSGASSGGSNGGGFVSAPPAGG
                     SGIPVIDPVQRFLKRTFEGIRFALASPGRAVRVFGMLGLFAAPLILWRRRGGIAPPGA
                     GP"
     gene            4328..>4770
                     /locus_tag="VM841_11835"
     CDS             4328..>4770
                     /locus_tag="VM841_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90912.1"
                     /translation="MRVRRRDIGFWETIGIVVALAVLSSIAVLPRFKTTVVAANDVPG
                     DTSQPGDTSQPGVGPTTGVPTSGAGPAKPGVPDASNLACAPGRNGGATDTGVTATSIK
                     LGATVVDTGIGAAFLSDVRYGMQAVINKVNADGGICGRRLDLVLK"
BASE COUNT          738 a         1631 c         1691 g          710 t
ORIGIN      
        1 ggtgggggct tcacgggtgg gggcgagttc ccctcgaccg gcccgctgct tccgggctac
       61 gaaaacacgc cgccggtccc ggtggacaag gttacggccg cggtcgaggc gctcggtgcg
      121 ggcgcaccgg ccggtgtcaa ggcgctctac ggcggcatgg cgttcgcgct cgcgatgctg
      181 gtcgccggac gacaagtgtt tcgcacgctg gcgagataga agggggatca accgttggac
      241 gaccgagacg accgagagcg acaccaagcc gcacccgagc cggaggccgg tggccggctt
      301 ctgctcggat ggattctgat cgttctcggc gtggtgatgg tgttcgccgg atggctcgga
      361 gtcagcggca accctcaggt cgccgtgcag cttccgtacc tgatctccgg cggcatcggc
      421 ggcctgctcg ccggcatcgt cggcgtcggg ctgctcgtgt ccgatgacat tcgaaaggat
      481 cgcgcgcgca tcggccgcct ggaagcggcc gtgctcgaga tgcgggagtt cgccgcggca
      541 caggccgagg cgatgggact agccggctct cgggagagcc gcgaggaggg gggatccgac
      601 gacgagacca ccgtgagacc gtaccgcgcg gccggcgagc agccggccgc caacggacgg
      661 atccgggcgg gagcgcgccg acgttgatgc cgctgcgacg cgaaggttcg atacgcggag
      721 caaaatcccc cggaaggggg aagggcatca ctcttcggcg gagaaggatc tttcagcacg
      781 ttttctcggc gcgtcgaagc gcgccactgg gagccaccgc gctgacgcgc gggagggaag
      841 ggggttgtac cgatgcgggt gaggatcacg aaggcggtgc tgaccgcggc gctcgcggcg
      901 atggcgatca tcggcaccac gccggcgggc gcggtgtccg cgtaccacat gaacggccag
      961 ggaacgatca cgatgcaagg tgccggtctg ccgtcctgct tgccggtcgt ccttcagggg
     1021 gactgcacac actcggatca gacattctcg tggggcaccc tgaccgtgca gggcgccggg
     1081 attctgcagg acgcggcggt cgccggacag taccgctgca agtcggaggg cgacgtgggc
     1141 ggcacggcgt tcgtcagcaa gatccgcggc aacacgtcga acttcgagct gacgttcaac
     1201 ttccagtgtc agaacctgtc cggcgtcggc ccgactttca tcaacggctg gttctccaac
     1261 atcaacggca gtgcggccgc tccgtatcgg agcgtcgccg gccatccgca cccgacaccg
     1321 gtcgagaagg gcgtgttcac cttcaaaggc tacttcacca agaccaacac ccacccgtac
     1381 aacatcaaca acggcaatgg cgacgcgatg acctgcgtgg gcggcttcgc cccgtcggga
     1441 ttcaacggcc tgaagatcac gacggccgcc ttcgcgggag cgtgcaccgc cgcggagtga
     1501 cgcagcgcga acgacacacg acggggggcg ttcgcgcccc ccgtcggcct tgaaaggacg
     1561 gcatgaccga gatgcgaaga atgactgagc ggaaagggcg gcggctggtt cggaccgccg
     1621 cgctgacgct cgcgctcgtg ttcctcgctc ctgcgggcct gccggttcac gccgcatcgg
     1681 atccgatcac gcaggcgggt gcgctcgaca catccatgat cggcgcacgc cgtcacctcg
     1741 acgcggaccc gtccgcgcgc cgggtcttcg agatcggtca gccggtcgat cagggtggca
     1801 tgcgagtcga cgcatgggac ctcgacacgc tccggccggc cggcagcgtc gggatcgccg
     1861 aggcgcaatc gggacagggc gggatgctca gcacggtcga cgagctgcgg caccggcttt
     1921 acgtgctcta ccccgtgaag gccgcggacg acaccgtcat ccggggcgtc ctcggacccg
     1981 gcatctccac cggtatctac gacgaagcca cgcgagcctt gttcggtgtc gcggccatcg
     2041 acacgttgcg catgaaggtg atcggctcgg gaacgctggc gttgcgcgtg ccgcccgtgc
     2101 cccgcgatac ctccgagtcc aacttcgtcg ggatcaaagc gatgtcgtac ttccgggaag
     2161 gcggccgcga tctgctctat ctcgcatccg aggtcccgac cggccaaggc aatctggtgg
     2221 tgtccaatca cacggttgcc attcatcaga tcgacgccca aaggctcatc gatcctgctt
     2281 cgaccctgac cgccgtcgac tggtcgatgc ccctgccgca gtgcgcgctc gtcatggccc
     2341 ggggaacgcc cggtgtgctc ttccggtcgg cgcgggagcc ggtcgtcgcg ctcccctgca
     2401 agcaaggagg cgccgccggt gtggcgggac aaccggcgga gacgccgggg atcgtgcgtg
     2461 tgcgtgtcac gccgggcgcg acccaaaccg atgtctcgac cttcgtcacg acgttccacg
     2521 cgatcagcgg caacctgcag tacggagtcg tcgcagccga tccgttgcgc gaacgcatct
     2581 tcatgcagat cgacggatcg aacaccgagg cggccatctg ggtcttcgac gttccgaccg
     2641 cgacctttat cgggatcgtg gcgctcccga ctgccttcgc gtcgggtggc acgcaggcgc
     2701 tcggcgtcga tccgtcgctc gcgcgcctgt acgcgatctc gactccgtcg ggcgagatct
     2761 tgcgcagcac cgtgctgatc acctccaccg acacgaccgt cgtcgatcaa gggctcatca
     2821 cgacgtttcg tccgcagccg gccggaagca agagcagacc ggttgtggac ccgatccgtc
     2881 accgcatcgt catgtcggcg gtcggggggt cgatcgtgct gcaggactcg ctgccgcagc
     2941 gaggcggcgc cggcgccgcg cccgatcccg acgcaggcac cgtcgatgcc gcagaggggg
     3001 cgggaaccgc ggtcaacttc ctcggcggcg gacaggccta cggggcgcgc atcagatggg
     3061 tcggtggcgt cggcggcgcc gaccgcaacc tcaacaccgt gctcgacccg cccccgagcg
     3121 agctgtcgcc ccagacgggc gggatcacgc cgccgccgcc cgctccgggc acacgcgacg
     3181 tgcacttcgc gcgcgtgctg cacacgacgc tgaccaacgg cgaagcgtcg gcgaaggcaa
     3241 ccggcgcgca acgagatcat gagaacaccg acgccgacct cggcggcatg gcgaactacc
     3301 ttcgccgctg gggcggcccc gatctcgccg accagcgctg gccgtacccg gaggtggact
     3361 gcaccgactt cggcggcgcc ggcgacacgc gaaacaagaa gagctacggc gccgaagtcg
     3421 cgtgccggca gagtgacgcg accgtgaccg cgctcgcgct cgacgagtcg ctctcgcagg
     3481 gcggcgtgat gcgcatcgcc caggcgcgcg cgcgaagcgt cgtggcacgc gacccgaagc
     3541 gcggcgtcgt gtcgatcgtg gagtcggaga tgctcggcgt gaacctcggc gggcgtgccg
     3601 gcatcggacg agtgctcgcg cgtgcggaga cgtgggcgag cgggcggccg aagtcgtcca
     3661 acggcgtcgg agcgggcgga tcgttccgtc gcatcttcga agacgtgtgg atcgtggacg
     3721 acgccggctc gcggcgcacc gtctgctcgg acctgtgcga ccccgaagag gtccgcgact
     3781 ccatcaatcg cgcgctcggc ctgcgcgcgc ggatcgactt gccggaaccg gagccggcgc
     3841 gcatgagcgg cacgcccggc ggcttcgaag cgctgatcgt gcgcgatccc ttcgagcgcg
     3901 cgaacgaagc atccgtcaac gaggaagccg acgcgcgccg gctcgaggtg cccgccatgg
     3961 tcgtgaccgt cttcgccgac ggccgcgtgc cgtcgcgcgt cgtgatctcg ctcgcgggcg
     4021 tctcggccga gtcgcattac gggatcttcc tgctggcgca gggaggcacg ttccggccgc
     4081 ccggctcggg cgcctcgtcc ggtggcagca acggcggcgg cttcgtctcc gctccgccgg
     4141 ccggcggctc gggcatcccg gtcatcgatc cggttcagcg attcctcaag cgcacgttcg
     4201 aaggaattcg gttcgcgctc gcgtccccgg gccgtgccgt gcgagtgttc ggcatgctgg
     4261 gtctgttcgc cgcgccgctg atcctgtggc ggcgtcgcgg cggcatcgcg cctccggggg
     4321 ccggcccgtg agagttcgca ggcgcgacat cgggttctgg gagacgatcg gcatcgtcgt
     4381 cgcgctcgcg gtgctgtcct cgatcgcggt gctcccgcgc ttcaagacca ccgtggtggc
     4441 tgccaacgac gtgccgggcg acacctcgca gccgggcgac acctcgcagc cgggtgtcgg
     4501 tccgacgacc ggcgtcccga cgtccggcgc cgggccggcg aagccgggcg tgccggacgc
     4561 gtcgaacctg gcatgcgcgc ccggccggaa cggcggcgcg accgacaccg gcgtcaccgc
     4621 cacgagcatc aagctcggcg ccaccgtcgt cgacaccggg atcggggcgg cgttcctgtc
     4681 cgacgtccga tacggcatgc aggccgtgat caacaaggtc aacgccgacg gcggcatctg
     4741 tggccgccgg ctcgacctgg tgctcaagga
//
LOCUS       DATLFO010000294        15444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_96899, whole genome shotgun sequence.
ACCESSION   DATLFO010000294 DATLFO010000000
VERSION     DATLFO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96899"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..806)
                     /locus_tag="VM841_11840"
     CDS             complement(3..806)
                     /locus_tag="VM841_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90913.1"
                     /translation="MKTRMLALASALIGAAVASSAAAGPVVPEVRCEPGTMAALLAPN
                     RSIEINRRGANVTARTGVLNPRTHSWSDAAEYSCPADQTERIVIDEDRIGGGPAEEPA
                     FRSVRVAPFTGGAAEAWGISEVEVEINAPSVLYAWMSPAAPRATVGRLDRTGVVPAAV
                     DINGDGDWDITGRIEVIAIIGTDGDDVIDASGARWGGPPPAPVHVLGGNGKDQIFGGS
                     WTSDIIDGGAGDDVIFSVDARSDVVRGGPGIDAARIDPRDSVSSVERRA"
     gene            complement(856..2634)
                     /gene="aspS"
                     /locus_tag="VM841_11845"
     CDS             complement(856..2634)
                     /gene="aspS"
                     /locus_tag="VM841_11845"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HVL90914.1"
                     /translation="MAEGEDDEMMRTHGAGELRASHAGQNVTLAGWVHHRRDHGKVIF
                     LDLRDASGTVQIVVHPDDAPDAYEAADALQREWCVLIDGAVAPRKPGTENPKLATGEI
                     ELKAKKVTVLSPAATPPFVIEDGVDVSEETRLKYRYLDLRRPEMQHAMRTRHAMIRGI
                     REFLDERDFVHIDTPNLTRSTPEGARDFLVPSRLHPGSFFALPQSPQLFKQLMMVAGL
                     ERYYQIARCFRDEDLRADRQLEFMQLDLEMSFCEEQDVQTLMEELMVRLWRDLLGVEL
                     TVPFPRMSYEEMMSRYGTDKADLRYGVELVDVGSVFASTQLGIFKRVLESGGIIKAIG
                     VPGGGEMSNADLRRLEQLAKERGAGGLAWVVYKDGGEIDSPLAKFLSEDEKTGLANAL
                     GIGAGDLALLAADKPSVVHTVLGAMRIHVAEMRNLVPDGQWAFCWMTDPPLFEWDEDE
                     KRYVSVHHPFTSPQGDDPIAAFADDPGTAKARAYDLVLNGVELGGGSIRIHRRDVQER
                     VLEALKRDPSEFDFLLEAFGYGAPPHGGIALGLDRMAMLIAGASSLRDVIAFPVTGDG
                     ADPLTGAPTPVATKQLDDLGLKIKPV"
     gene            complement(2607..3878)
                     /gene="hisS"
                     /locus_tag="VM841_11850"
     CDS             complement(2607..3878)
                     /gene="hisS"
                     /locus_tag="VM841_11850"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVL90915.1"
                     /translation="MSATPQPPRGMQDLLPPVAERFQMHEQRTGALFERAGYRRIITP
                     MFEDTALFLRGVGESSDIVNKEMYTFTDRSENSLTLRPEGTAPVMRAVLSNNLHSQGL
                     PIKVWYSAAMFRYDRPQKGRYRQHHQLGIEAIGSDDPAIDAEVIAIAQRMLDGAGVTG
                     TRLLLNSIGHPGCRADYMPKLTAFLEARRERLDEDCKRRMEQNPLRVFDCKNEDDQKI
                     LADAPTIEQELCADCRAHFDAVQAHLRGIGIEFVVAPRLVRGLDYYTRTAFEFQTDLL
                     EAAQSTVCGGGRYDLLSESIGGPPLRGIGFGSGIERILIAQEAAGNAPPAVPLECFVI
                     ALGEDERDDAWRLVWALRDAGRSADLPFAPKSFKAGLKHADRVGARYAALIGPSEREA
                     GACAMRDMSTGEQVQVPLDEAATWLKEKTTR"
     gene            complement(3875..4543)
                     /locus_tag="VM841_11855"
     CDS             complement(3875..4543)
                     /locus_tag="VM841_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL90916.1"
                     /translation="MIVEGFTTGVFQSNCYIAAAKDAESAVVIDPGQDAAGPLLERLA
                     ALGLRCEAVLLTHGHLDHIWNAKEVAEAAGVEAWIHPDDLWLLDDPGAALGRMGFGKL
                     SIDPPERVAHLSDGDVLRYGGLSLTARHTPGHTPGHCVFLTDGLVFSGDLIFAGSVGR
                     TDFPRGSMEALMDSIRRVVLPLADETTILSGHGPETTVGTERTSNPFVVADASGDLPR
                     LLGL"
     gene            complement(4641..5096)
                     /gene="dtd"
                     /locus_tag="VM841_11860"
     CDS             complement(4641..5096)
                     /gene="dtd"
                     /locus_tag="VM841_11860"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HVL90917.1"
                     /translation="MRAVVQRVRRACVRVAGQEISRIEEGLLVLLGVSTEDTGSDAAS
                     IAVKMAAKITRLRVFPDDAGVMNRSVVEHGGAVLAVSQFTLYGDVRKGNRPSYVKAAP
                     GEAARPVYEAFCDAMRAEGLTVRQGSFGADMDVELVNWGPVTIVLESGA"
     gene            complement(5097..7355)
                     /locus_tag="VM841_11865"
     CDS             complement(5097..7355)
                     /locus_tag="VM841_11865"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HVL90918.1"
                     /translation="MSDRLAHEDSSPPRRRGVLARLRPVEPTPEAGFEGVARIIRTHS
                     PKADLKLIERAYTFAAASHADQYRASGEPFIEHPAGVCAILAELGMDTTTLVSALLHD
                     VVEDTPLSLADIEREFGAEVRLIIDGLTKLDKIQFHSREQQQAENLRKMILAMARDIR
                     VILIKLADRLHNMRTIGHVPREKQEKKARETLDVFAPLAHRLGIYQVKWQLEDLAFAT
                     LHPKQFDEIVRMVEDRQPERDALLDRVIVALGDALKESRIKAEVTGRPKHYWSIYNKM
                     VLRGKEFGEIFDLVGVRVLVESVRDCYATLGVIHGRWKPVPGRVKDYIAMPKFNMYRS
                     LHTTVVGPEGKPLEIQIRSQEMHRMAEYGVAAHWKYKGPGKAGRTDQDFAWLRQMLDW
                     QREMSDPEEFMDSLRIDLYQDEVFVFTPKGDVMPFPRGATPIDFAYSIHTDVGHRTVG
                     AKVNGRLVPLDYRMESGDTVEIVTSKAPSAAPHQDWLKIVRTTRAKNKIRQWFSRERR
                     VDAMERGREELARALRKAGMPVKDTLNSSELGAIAAEFGLAGVDALFAHIGESHSSTQ
                     NVIERLRHELSEDDEPVVAAPRPMRMRPASQHGVMVKGIEDVLVRLARCCTPVPGDAI
                     IGFITRGRGVSVHRVDCPNGKSLQAEGERLVEVAWDTRTGAAFPVTLQVEALDRTKLL
                     RDVTIAISDLGVNITATTSNTNVKTRIALLRFTIELGDTSHLDHILAAVKRVDGVFDA
                     YRVVPGRSRARA"
     gene            complement(7495..8364)
                     /locus_tag="VM841_11870"
     CDS             complement(7495..8364)
                     /locus_tag="VM841_11870"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HVL90919.1"
                     /translation="MTARFRTVVVATLTAATLTALLFGSAARAEQICVLGPAGKPVCI
                     DVEGQFNPLCKEGRPTHGETGQEGGDPLCPNGQAECIAYGMYLRVDPTTGLPGGATSH
                     LARSHAQAQAGAWNGWGYGSAASDASTQRADVPGALGEGVVESSCRAFSYGHGQGFAQ
                     NYAAGTADAARLNLNLTSYGVPVTVSTSVLHEEGNASTGFVGYNTADIATVTIASPWF
                     PAINVPLAAAPNTAIPLPGGLGTLYLNEQFAGISPWGCATFAGDALRLVVNRPGIGGQ
                     ITLIMSWVANAAC"
     gene            complement(8511..9044)
                     /locus_tag="VM841_11875"
     CDS             complement(8511..9044)
                     /locus_tag="VM841_11875"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HVL90920.1"
                     /translation="MGDGRHLQILRDRIRDIPDFPEPGIVFKDITPLLADHVAFSTAI
                     DAIVVHFGRGTVDKVVGVEARGFIVASPVAYHFGAGFVPVRKAGKLPWQTQAESYALE
                     YGEATLEIHGDAIAPGERVAIVDDVLATGGTASACAKLVERLGGTVVGLAFLVELEFL
                     KGRAVLDGYNCLSLVTY"
     gene            complement(9049..10200)
                     /gene="secF"
                     /locus_tag="VM841_11880"
     CDS             complement(9049..10200)
                     /gene="secF"
                     /locus_tag="VM841_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVL90921.1"
                     /translation="MGLVRGYKALAKGESDFNIIGRWRTWGIVSLALVVVSLGGLLLR
                     DLNLSLAFEGGSAFSAPISEGKDPTVPEISAAMTDAGLTNPEVQIARSADGSRIIRVE
                     AKTVGVLEPVVDKLAGISGAERDAISVQEVGPTWGSQISSKALRALIVFLILVVIYIS
                     IRFEPKVALASIVALIHDLIFAVGIYALVGFEVSPATVVAYLTIMGYSLYDSVVVFDK
                     VRENQVLVTGTSRMTYSDMVNKSVNQTLMRSINTSLSSLLPVGALLFVGVYLFGADTL
                     KDLALALFLGIAVGSYSSVFVGPPLLALLKEREPRYKQLRARASRGMTPAAATAGPAS
                     SGGATGPAPDDEDDTEPPAPRSTRAQPGPGRQTTVAPRGRKKSRKKRRR"
     gene            complement(10200..11834)
                     /gene="secD"
                     /locus_tag="VM841_11885"
     CDS             complement(10200..11834)
                     /gene="secD"
                     /locus_tag="VM841_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVL90922.1"
                     /translation="MAKSLRRKYWTSILVMLVIVLGIWSAVGFGCTTKTVTVDGKDTV
                     VCEGPMEAQLGLDLQGGISVVLAPKGEASSDSLDKAVDIIRQRVDALGVAEPDISRQG
                     NNILVEIPGIKDRQAALDTIGRTAELRMRPVKSILAPGTQEWAASPPEDCTRPFASAT
                     QSASPAASPKPTASPSPTASPGSTPEAGEGEEDPTKDITLCARAKTPDGDDLPDDSWQ
                     KVVMGPARLVGSDIADSRAEIAGGGAAITAAGWQVTLNLTSEGAKKFEEITGELACNP
                     SGDPKRQFAIVLDGIVESHPQMGEDVQCEQGISGGTAVITGNFSEREAKNLALVLRFG
                     ALPIALEAQEINEISPTLGRDSLRGGLMAGLIGLAMTMIYVMFFYRVLGLVIWFGLLL
                     HAAFTVGVVILLGNTVGFALTLAGIAGLIVSLGIAADSFIVYFERLKDEVHTGKSVRA
                     SVDRAWSSAWRTILAADLVTALAAGALYVLAVGSVRGFALMLGLAVTLDLIISYMVMH
                     PVVWLLAQTKFFNESKRLGIKRVVGDATPSLAGGQR"
     gene            complement(11872..12189)
                     /gene="yajC"
                     /locus_tag="VM841_11890"
     CDS             complement(11872..12189)
                     /gene="yajC"
                     /locus_tag="VM841_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVL90923.1"
                     /translation="MINFFAQSGSESGQYTSLIFLALLFGAMYFLMIRPQQRRRKQYL
                     ELLGSLQVGDEIETVAGIFGTIRRLDDDHAYVEVADGLTLKMSRGAIRRKISADTGTD
                     AAV"
     gene            complement(12362..13372)
                     /gene="ruvB"
                     /locus_tag="VM841_11895"
     CDS             complement(12362..13372)
                     /gene="ruvB"
                     /locus_tag="VM841_11895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVL90924.1"
                     /translation="MSEILTGSVLPEEHDFDAALRPKRLEEFVGQPRVKEQLSLILDA
                     ALARGAAPDHLLFSGPPGLGKTSLAHIVASEIGSQIRTTSGPAIERAGDLAAIVTNLE
                     PGDVLFIDEIHRLRTQVEEILYAAMEDFTLDVVIGKGPAARSIRIDLPRFTLIGATTR
                     TGLLTGPLRDRFGFAARLDYYDAPDLGEILHRSGGLLEIPLDEEGATEIAGRARGTPR
                     VANRLLRRVRDFADVRADGRIPGDVARDALAVFEVDELGLDRLDIALLRAVVEKFGGG
                     PVGVATIAAAVHEEPETIEDVAEPYLLRLGFLKRTPRGRVATEAAYRHLGLNRPAAQT
                     LL"
     gene            complement(13369..13965)
                     /gene="ruvA"
                     /locus_tag="VM841_11900"
     CDS             complement(13369..13965)
                     /gene="ruvA"
                     /locus_tag="VM841_11900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HVL90925.1"
                     /translation="MIAMLSGVVAATGPTGVVLDVGGVGYRLHCPASSIAAIGRTGSK
                     ATLHTHLHVREDAMTLYGFPTSDERDVFETLIAVNGIGPKAALAILSAYSPDALRKAV
                     AAEDLDALTLVPGIGKKTAGRMILELKEKLGGEASFAGVSLPEDARPAFEEAREALLS
                     LGYSTTEAREALEAVAGSAASGSEELLRLALKTLGARR"
     gene            complement(13962..14489)
                     /gene="ruvC"
                     /locus_tag="VM841_11905"
     CDS             complement(13962..14489)
                     /gene="ruvC"
                     /locus_tag="VM841_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVL90926.1"
                     /translation="MFERGAPVRIIGFDPGLTRAGYGIVEERGGRLRSVEHGTLRAAG
                     ETPAEQLATLRIRLLEAMERTGPAAMAIERIFFNRNVRTAARTLQASGVAMCVAAERG
                     LPVYEYGPLEVKRAVVGVGDADKKQVMFMIRRILSLSADLTEPDAADALALAVAALHG
                     RPWLTAPAALRGAAR"
     gene            complement(14572..>15444)
                     /locus_tag="VM841_11910"
     CDS             complement(14572..>15444)
                     /locus_tag="VM841_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90927.1"
                     /translation="TVRSAAFDPAVVTAPGGDLIRWTVETGNHTIHAYAGATFAHSLV
                     QQGDVVSTTFSGGTALYRCEFHSRIDARSLDCQGMCGAITDRTSAPAPAAITAPADGA
                     TIPTRIVRITGTAEPQTHAEIRSAGAPIGRALAGNDGRWAIDLDFLRGTHTIVARATH
                     VNGRSAADSAPVTFHVTADTEPPAAFVGGNQLQAGLRKPGSVRGSATDDIALAWVDVR
                     IARPASMPFGLEAPPKIVRVPASGTFWQWQVDGEVPPGVWAVSARATDFAGNVGEWSE
                     AVVVVFVPAFPIRE"
BASE COUNT         2361 a         5267 c         5384 g         2432 t
ORIGIN      
        1 attcaggcgc ggcgctcgac cgaggagacc gagtcgcgag ggtcgatccg cgcggcgtcg
       61 atgccggggc ccccccggac gacgtcgctg cgggcgtcca ccgagaagat gacgtcgtcg
      121 ccggcacctc cgtcgatgat atcgctcgtc cacgagccac cgaagatctg gtcctttccg
      181 ttccccccga gcacgtggac cggggccgga ggaggaccac cccatcgagc accggatgca
      241 tcgatcacgt cgtcaccgtc ggtcccgatg atggcgatga cctcgatgcg gccggtgatg
      301 tcccagtcgc cgtcgccgtt gatgtcaacc gcggcaggca ccacgccggt tcggtccagc
      361 cggccgaccg tcgcgcgcgg ggcagccggg ctcatccatg cgtacagcac cgatggcgcg
      421 ttgatctcga cctcgacctc ggagatgccc cacgcttcgg cggcgccgcc ggtgaacgga
      481 gccacgcgaa ccgaacggaa cgccggctcc tccgccggtc ccccgccgat ccggtcctcg
      541 tcgatgacga tccgttcggt ctgatcggca ggacaggagt attccgcggc gtcgctccac
      601 gaatgggtac gaggattcaa cacgccggtc cgggcggtca cgttcgcgcc ccggcggttg
      661 atctcgatgc ttcgattcgg cgcgagcagg gctgccatgg ttccgggttc gcagcgaact
      721 tcgggaacga ccggcccggc agcggccgac gatgcgacgg cggcgccgat cagcgccgac
      781 gccaacgcaa gcatccgtgt cttcatgacc ggaccaacga ttcgtccatc ccgcccgttg
      841 cggtgcggtt ccgccttaga ccggtttgat cttcaggccg agatcgtcga gctgcttggt
      901 tgcgaccggc gtcggcgcgc cggtcagggg gtcggcgccg tcgccggtga ccgggaacgc
      961 gatcacgtcg cgcagcgacg acgcgccggc gatcagcatc gccatccggt ccaggcccag
     1021 cgcgatcccg ccgtgcggcg gcgcgccgta cccgaacgcc tccagcagga agtcgaactc
     1081 gctcgggtcg cgcttgagcg cctcgagcac gcgctcctgc acgtcgcggc gatggatacg
     1141 gatgctgccg cctcccagct cgacgccgtt gagcaccaaa tcgtacgcgc gcgccttcgc
     1201 agtgcccggg tcgtcggcga acgcggcgat cggatcgtcg ccctgcggcg aggtgaacgg
     1261 atggtgcacg ctgacgtatc gcttctcgtc ctcgtcccac tcgaacagcg gcgggtcggt
     1321 catccagcag aacgcccact gcccgtcggg gaccaggttg cgcatctcgg cgacgtgaat
     1381 gcgcatcgcg cccaggacgg tgtgcacgac cgaaggcttg tcggccgcga gcagcgccag
     1441 atcgcccgcg ccgatgccga gtgcgttcgc cagcccggtc ttttcatcct cggacaggaa
     1501 cttcgcaagc ggcgagtcga tctcgccgcc gtctttgtag acgacccagg ccagaccgcc
     1561 cgcgccgcgc tccttggcga gctgctccag gcggcggagg tcggcgttgc tcatctcgcc
     1621 gccgcccggc acgccgatcg ccttgatgat gccgccggat tccagcacgc gcttgaagat
     1681 cccgagctgc gtgctcgcga aaaccgaacc gacatcgacg agttcgacgc cgtatcgaag
     1741 gtcggcctta tcggtgccgt accggctcat catctcttcg tagctcatgc gcgggaacgg
     1801 cacggtcagt tcgacaccga gcagatcgcg ccacagtcgg accatgagct cctccatcag
     1861 cgtctgcacg tcctgctctt cgcaaaacga catctccagg tcgagctgca tgaactcaag
     1921 ctggcggtcg gcgcgaaggt cctcgtcgcg gaagcagcgc gcgatctggt agtagcgctc
     1981 gaggccggcg accatcatga gctgtttgaa cagctgcggc gactgcggca gcgcgaagaa
     2041 cgagcccgga tgcaggcggg acggaaccag gaagtcgcgc gcgccctcgg gcgtggagcg
     2101 cgtcaggttc ggcgtgtcga tgtgcacgaa gtcacgctcg tcgaggaatt cgcggatacc
     2161 gcggatcatc gcgtggcgcg tccgcatcgc gtgctgcatc tcgggacgcc gaagatccag
     2221 gtagcggtac ttgagccggg tctcctcgga cacgtccacg ccgtcctcga tcacgaacgg
     2281 cggtgtcgcc gcgggcgaca ggaccgtcac cttcttggct ttcagctcga tctcgccggt
     2341 ggcgagcttg gggttctcgg tgccgggctt acggggcgcg accgcgccgt cgatcagcac
     2401 gcaccattcg cgctggagcg cgtcggcggc ctcgtaggcg tcgggcgcgt cgtcggggtg
     2461 cacgacgatc tgcacggtgc cgcttgcgtc gcgaaggtcg aggaagatca ccttgccgtg
     2521 gtcgcggcgg tgatggaccc acccggccag cgtgacgttc tgcccggcgt gggaggctcg
     2581 cagctcgccg gctccgtgcg tgcgcatcat ctcgtcgtct tctccttcag ccacgtcgcg
     2641 gcttcgtcca agggaacctg aacctgctca ccggtgctca tgtcgcgcat cgcgcacgcg
     2701 ccggcctcgc gctcggaagg accgatcagc gcggcgtagc gcgcgccgac gcggtcggcg
     2761 tgcttgagcc cggccttgaa gctcttggga gcgaacggca ggtcggccga gcgccccgcg
     2821 tcgcgcagcg cccagaccag ccgccatgcg tcgtcgcgct cgtcctcgcc cagcgcgatc
     2881 acgaagcact ccaggggcac ggccggtggc gcgttgccgg ccgcctcctg cgcgatcagg
     2941 attcgctcga tgcccgaacc gaatccgatg ccccgcagcg gcggtccgcc gatggattcg
     3001 ctgagcaggt cgtagcggcc gccgccgcac acggtggatt gcgcggcttc gagcagatcg
     3061 gtctggaact cgaaagcggt ccgagtgtag tagtcgagcc cgcgcaccag ccgcggtgcg
     3121 acgacgaact cgatcccaat cccacgaagg tgggcctgaa cggcgtcgaa gtgcgcgcgg
     3181 cagtcggcgc acagctcctg ctcgatcgtg ggcgcatcgg cgaggatctt ctggtcgtcc
     3241 tcgttcttgc agtcgaacac gcgcagcggg ttctgctcca tgcggcgttt gcagtcctcg
     3301 tcgaggcgct cgcgccgggc ctcaaggaac gccgtcagct tcggcatgta gtcggcgcgg
     3361 cagccgggat ggccgatgct gttgagcagc agccgggtgc cggtcacgcc cgcaccgtcg
     3421 agcatgcgct gggcgatcgc gatcacctcg gcgtcgatgg ccggatcgtc gctgccgatc
     3481 gcctcgatgc cgagctggtg gtgctgccgg tagcgcccct tctgcggccg gtcgtagcgg
     3541 aacatcgcgg ccgagtacca gaccttgatc ggcaaaccct gcgaatgcag gttgttcgac
     3601 aacacggcgc gcatcaccgg cgcggtcccc tccggccgaa gcgtcagcga gttctcgctg
     3661 cgatcggtga aggtgtacat ctccttgttc acgatgtcgc tcgattcgcc gacgccgcga
     3721 aggaacagcg cggtgtcctc gaacatcggc gtgatgatgc gacggtagcc ggcgcgctcg
     3781 aacagggctc cggtgcgctg ctcgtgcatc tggaagcgct cggcgacggg cgggaggaga
     3841 tcctgcatcc cgcggggcgg ctgcggcgtg gcgctcatag gccgagcagg cgcggaaggt
     3901 cgccgctcgc gtcggcgacc acgaacgggt tcgacgtgcg ctcggtgccg accgtggtct
     3961 cggggccgtg gcccgacagg atcgtggttt cgtcggcgag cggaagcacg acccggcgga
     4021 tcgagtccat cagtgcctcc atcgaaccgc gcgggaagtc ggtgcggccg acggacccgg
     4081 cgaagatcag atcgccgctg aagacgagcc cgtcggtgag gaagacgcag tggccgggcg
     4141 tgtggccggg cgtgtgtcgc gcggtcagtg acaggccgcc gtaccggagc acgtcgccgt
     4201 cgctcaggtg cgcgacccgc tccggcggat cgatgctgag cttgccgaat cccatgcggc
     4261 cgagcgcggc accgggatcg tcgagaagcc acaggtcgtc gggatgaatc caggcttcga
     4321 ccccggcggc ctcggcgacc tccttcgcgt tccagatgtg atccagatgg ccgtgcgtga
     4381 gcagcaccgc ttcgcagcga agcccgagcg ctgcgaggcg ttcgagcagc gggccggccg
     4441 cgtcctggcc cgggtcgatc acgaccgcgg actcggcatc ctttgcggct gcgatgtagc
     4501 agttcgactg aaagacgccc gtggtgaagc cttcgacgat catcggcgca cagactagcc
     4561 gccgggccgg gccgatgcga agtcgctttt gcgaagtgag cgggttcggc ctcgggcgag
     4621 atcgcagggg ccggccccgg tcatgcgccc gactcgagca cgatcgtcac cggcccccag
     4681 ttgacgagct cgacgtccat gtcggcgccg aagcttccct gcctgaccgt gagcccctcg
     4741 gcccgcatcg cgtcgcagaa cgcttcgtag accggccgcg cggcctctcc gggggccgcc
     4801 ttgacatagg aggggcggtt tcccttgcgc acatcgccgt agagcgtgaa ctggctgacg
     4861 gcgagcaccg cgccgccgtg ctcgacgacc gaacggttca tgactccggc gtcgtcgggg
     4921 aagacccgca gccgggtgat cttggcggcc atcttcacgg cgatcgatgc ggcatccgat
     4981 ccggtgtcct cggtcgagac tcccagcaga accagcagcc cttcttcgat gcgggagatc
     5041 tcctggccgg cgacacggac gcaggcgcgg cgcacgcgct gcacgacggc gcgcatctag
     5101 gcgcgagcgc gcgagcggcc cggcacgacg cgataggcgt cgaagacgcc gtccacgcgc
     5161 ttgaccgcgg ccaggatgtg gtccaggtgc gaggtgtcac cgagctcgat cgtgaatcgc
     5221 agcagcgcga tgcgcgtctt gacgttggtg ttcgaggtgg tcgccgtgat gttcacgccg
     5281 aggtccgaga tcgcgatggt gacgtcgcga agcagcttgg tgcggtcgag ggcctcgacc
     5341 tggagcgtga ccgggaacgc ggcgccggtc cgcgtgtccc aggcaacctc gaccagccgc
     5401 tcgccctcgg cctgcaacga tttgccgttc gggcagtcga cgcggtgcac ggacacaccg
     5461 cggccgcgcg tgatgaaacc gatgatggcg tcccccggca ccggcgtgca gcagcgcgca
     5521 aggcggacca gaacgtcctc gatgcctttg accatgacgc cgtgctgcga cgccggacgc
     5581 atgcgcatcg ggcggggcgc ggcaacgacc ggctcgtcgt cctccgacag ttcgtggcgc
     5641 agccgctcga tcacgttctg ggtgctggag tggctctcgc cgatgtgggc gaacagcgcg
     5701 tcgacgcccg cgagcccgaa ctcggccgcg atcgcaccca gctccgagga gttgagcgtg
     5761 tccttgaccg gcatgccggc cttgcgcagc gcccgggcaa gctcctcgcg gccgcgctcc
     5821 atcgcgtcga cgcgccgctc gcggctgaac cactgccgga tcttgttctt ggcgcgggtg
     5881 gtgcgcacga tcttgagcca gtcttggtgc ggcgcggcgc tgggtgcttt cgaggtgacg
     5941 atctcgacgg tgtcgccgga ctccatccgg tagtcgagcg gaaccagccg gccgttcacc
     6001 ttcgccccaa cggtgcgatg cccgacgtcg gtgtggatcg agtaggcgaa gtcgatcggg
     6061 gtggcgccgc gcgggaacgg catcacgtcg cctttcggcg tgaagacgaa cacctcgtcc
     6121 tggtagaggt cgatccggag gctgtccatg aactcctccg gatccgacat ctcgcgttgc
     6181 cagtcgagca tctgccgcag ccaggcgaag tcctggtcgg tgcgaccggc cttgccgggg
     6241 cccttgtact tccagtgggc ggcgacgccg tactcggcca tgcggtgcat ctcttgtgag
     6301 cggatctgga tctcgagcgg cttgccctcg gggccgacga cggtggtgtg cagcgaccgg
     6361 tacatgttga acttcggcat cgcgatgtag tccttcacgc ggcccgggac cggcttccag
     6421 cggccgtgga tgactccgag ggtcgcgtag cagtcgcgaa ccgactcgac gagcacacgc
     6481 acgccgacca gatcgaagat ctcgccgaac tccttgccgc gcaggaccat cttgttgtag
     6541 atcgaccaat agtgcttcgg ccggccggtg acctcggctt tgatgcggga ctccttcagc
     6601 gcgtcaccga gcgcgacgat cacccggtcc agcagcgcgt cccgctcggg ctggcggtcc
     6661 tccaccatgc gaacgatctc gtcgaactgc ttcggatgca gcgtcgcgaa ggcaaggtcc
     6721 tcgagctgcc acttgacctg atagatgccc agccggtgcg ccaggggcgc gaacacgtcg
     6781 agggtctcac gcgccttctt ctcctgcttc tcgcgcggaa cgtggccgat ggtccgcatg
     6841 ttgtggagcc ggtcggcgag cttgatcagg atgacgcgga tgtcgcgcgc catcgccagg
     6901 atcatcttgc gcaggttctc ggcctgctgc tgctcccgcg agtgaaactg gatcttgtcg
     6961 agcttggtga gcccgtcgat gatcagccgc acctcggcgc cgaattcccg ctcgatgtcg
     7021 gccagcgaca gcggcgtgtc ctcgaccacg tcgtgcagca gggccgacac cagggtcgtg
     7081 gtgtccatgc cgagctccgc caggatcgcg cagacgccgg ccggatgctc gatgaagggc
     7141 tcacccgagg cccggtactg gtcggcgtgc gaggcggccg cgaacgtgta ggcccgctcg
     7201 atgagcttca ggtccgcttt cgggctgtgc gtccgaatga tgcgcgcgac gccctcaaat
     7261 cccgcttcgg gtgtcggctc gaccggccgg agccgcgcca ggaccccgcg tcgccgcggc
     7321 ggtgacgagt cttcgtgcgc gagccggtcc gacatggaga cattgtaggg gactgcccgc
     7381 gaggcgacac cgatccttcc gatcgcgttc aggcgtccca gcggcgccgg ctcttcttgc
     7441 gagccggcgc cgccgatggg aggctccgca ttcgtcatgc ggagtgggcc gttgctagca
     7501 ggccgcgttc gcgacccagg acatgatcag cgtgatctga cccccgatcc cgggacggtt
     7561 gaccaccagc cgcagcgcgt cgccggcgaa ggttgcgcag ccccacgggc tgatgccggc
     7621 gaactgctcg ttgaggtaga gcgttcccag gcccccgggc agcgggatcg cggtgttggg
     7681 cgcggcagcc agcgggacgt tgatcgccgg gaaccagggc gaggcgatcg tgaccgtcgc
     7741 gatgtcggcc gtgttgtagc cgacgaagcc cgtcgaagcg ttgccctctt cgtgcagcac
     7801 gctggtgctc accgtaaccg gcacgccgta ggaggtgagg ttgaggttca gacgggccgc
     7861 gtccgcggtc ccggctgcgt agttctgcgc gaagccctgg ccgtgtccgt aggagaaggc
     7921 acggcagctc gattcgacga cgccttcgcc gagcgctccg ggaacgtcgg cgcgctgcgt
     7981 ggacgcatcc gacgccgcgc tgccgtatcc ccaaccgttc cacgctccgg cttgggcctg
     8041 cgcgtggctg cgtgccaggt gcgaggtggc gccgccgggc agcccggtcg tcgggtcgac
     8101 ccgcaggtac atgccgtagg cgatgcactc ggcctgaccg ttcgggcaga gcgggtcgcc
     8161 tccctcctgt cctgtctcgc cgtgcgtcgg gcggccctcc ttgcagagcg ggttgaactg
     8221 gccttccacg tcgatgcaga cgggcttgcc ggccggcccc aggacgcaga tctgttcggc
     8281 gcgggcggcc gagccgaaca gcagtgccgt gagcgtggcg gcggtcagcg tcgcgacgac
     8341 gacggtgcgg aatcgagcgg tcatgttccc tccctcaggt ccggggcccc ccctttgggc
     8401 gccctcgcct tacccgcgaa cagcatcgcc gggacgcggg ggttgcccct aggccggaag
     8461 accggaggga atgtgtcgtt ttcacgttcg cgcgtccgcg cgaaacggac tcagtacgtg
     8521 accagggaga ggcagttgta gccgtccagg acggcgcggc ccttcaggaa ctccagctcg
     8581 accaggaatg cgaggcccac gacggtgccg ccgagccgct cgaccagctt cgcgcacgcc
     8641 gacgcggtgc cgcccgtggc gagcacgtcg tccacgatcg cgacgcgttc gccgggcgcg
     8701 atcgcatcac catggatttc gagcgtcgcc tcgccgtatt cgagcgcgta ggactcggcc
     8761 tgcgtctgcc acggcagctt gccggctttg cgaaccggca cgaagcccgc tccgaagtgg
     8821 taggcgaccg gagaggcgac gatgaacccg cgcgcctcga cacccacgac cttgtcgacc
     8881 gtgccgcggc cgaaatgcac cacgatcgcg tcgatcgccg tcgagaacgc gacgtgatcg
     8941 gcgagcagcg gcgtgatgtc cttgaagacg atcccgggct cgggaaagtc ggggatgtcg
     9001 cggatccggt cgcgcaggat ctgcaaatgc cgaccgtcgc ccatcgggtc agcgccgccg
     9061 cttcttgcgg ctcttctttc ggcctcgcgg cgcgacggtg gtctgccgtc cgggtccggg
     9121 ctgcgctcgc gtggatctcg gcgcgggggg ctccgtgtcg tcctcgtcgt cgggagccgg
     9181 cccggtcgcc cccccggagg aggccggacc ggccgtggcc gcggccggcg tcatgccgcg
     9241 tgaggcgcgc gcgcgaagct gcttgtaacg cggctcgcgc tccttgagaa gtgcgagcag
     9301 cggcggaccg acgaacaccg acgagtacga gccgacggcg atgccgagga acagcgcgag
     9361 cgccaggtcc ttgagcgtgt cggcaccgaa tagatagacg ccgacgaaca gcagcgcccc
     9421 gacgggcagc agcgacgaca gggacgtgtt gatcgagcgc atcagcgtct ggttgaccga
     9481 cttgttgacc atgtcgctgt aggtcatgcg cgaggtgccg gtgaccagca cctggttttc
     9541 acggaccttg tcgaagacca cgaccgagtc gtagagcgaa taccccatga tcgtcagata
     9601 ggccacaacg gtggcggggc tgacctcgaa gcccaccaga gcatagatgc cgacggcgaa
     9661 gatgaggtcg tggatcagcg ccacgatgga ggccagggcg accttgggct cgaaccggat
     9721 cgagatgtag atcacgacca ggatcaggaa cacgatcagg gcccgaagcg ccttgctgga
     9781 gatctggctt ccccacgtgg ggccgacctc ctggacgctg atcgcgtccc gttccgcgcc
     9841 cgagatgccg gcgagcttgt cgacgacggg ctcgagcacg ccgaccgtct tggcttccac
     9901 gcggatgatg cgggagccgt cggccgagcg ggcgatctgc acctccgggt tggtgagccc
     9961 ggcgtccgtc atcgctgcgg agatttcggg caccgtcgga tccttgcctt cgctgatcgg
    10021 cgccgagaag gcagacccgc cctcgaaggc gaggctgagg ttgaggtcgc gaaggagcag
    10081 cccgccgagg ctcacgacca caagggcgag cgaaacgatc ccccaggtgc gccagcggcc
    10141 gatgatgttg aagtcggact cgcccttggc cagggccttg tatccgcgta cgaggcccat
    10201 cagcgctgcc ctcccgcgag cgacggagtc gcgtcgccga cgacgcgctt gatgccgagc
    10261 cgcttggact cgttgaagaa cttggtctgg gccagaagcc acacgaccgg gtgcatgacc
    10321 atgtacgaga tgatcaggtc caaggtcacg gccaggccga gcatgagcgc gaagccgcgc
    10381 acgctaccga ccgccagcac gtacagggct ccggccgcga gcgcggtgac gaggtcggcc
    10441 gcgaggatcg tgcgccaagc gctggaccag gcccggtcca ccgaggcgcg aaccgacttg
    10501 ccggtgtgga cctcgtcctt gagccgttcg aagtagacga tgaaggagtc ggccgcgatc
    10561 cccagcgaca cgatcaggcc ggcgatgccg gcgagcgtga gcgcaaagcc caccgtgttg
    10621 ccgagcagga tcacgacgcc gacggtgaag gcggcgtgca gcagcagccc gaaccagatc
    10681 accaggccga gcacgcggta gaagaacatg acgtagatca tcgtcatcgc caaaccgatc
    10741 aggcccgcca tcaggccgcc gcgcagcgag tcgcggccca gcgtcggcga gatctcgttg
    10801 atctcctggg cttcgagcgc gatcggcagc gcaccgaagc gaagcaccag cgcgaggttc
    10861 ttggcctcgc gttcgctgaa gtttccggtg atgaccgcgg tgccaccgct gatgccctgc
    10921 tcgcattgga cgtcctcgcc catctgcggg tgcgactcga cgatgccgtc gagcacgatc
    10981 gcgaactggc gcttcgggtc gccgctcggg ttgcaggcga gctcgccggt gatctcctcg
    11041 aacttcttgg cgccctccga ggtgaggttg agcgtgacct gccagcccgc cgcggtgatc
    11101 gccgcgccgc caccggcgat ctcggcacgc gagtcggcga tgtcggagcc gactagccgg
    11161 gccggtccca tcacgacctt ctgccaggag tcatcgggca gatcgtcgcc gtcgggcgtc
    11221 ttcgcgcggg cgcagagcgt gatgtccttc gtcgggtctt cctcgccctc gcccgcctcc
    11281 ggcgtggagc ccgggcttgc ggttgggctg gggctcgcgg tgggcttggg gctcgcagcc
    11341 gggctcgcag actgcgtggc cgacgcgaac ggacgtgtgc agtcctcggg cggcgaagca
    11401 gcccattcct gcgtgccggg cgcaaggatg ctcttgaccg gccgcatgcg cagctcggcg
    11461 gtgcggccga tcgtgtcgag cgctgcctgc cggtccttga tgcccgggat ctccaccagg
    11521 atgttgttgc cctggcgcga aatgtcgggc tcggccacgc cgagcgcgtc gacgcgctga
    11581 cggatgatgt cgacggcctt gtcgagcgag tcgctgctcg cctcaccctt cggagcgagc
    11641 acaacggaga ttccgccctg aaggtcgaga ccgagctggg cctccatcgg gccctcgcac
    11701 acaaccgtgt ccttgccgtc aacggtgacg gtcttggtcg tgcagccaaa gccgacggcc
    11761 gaccagatcc cgaggacgat gaccagcatc acgaggatgg aggtccaata cttgcgacgc
    11821 aagctcttcg ccatcagcct gccgccttgc gaacgatcgc gacccgctcg ctcatacggc
    11881 ggcgtccgtt ccggtgtcgg cggagatctt gcggcggatc gcgccgcgac tcatcttgag
    11941 cgtgagcccg tcggcgacct cgacgtaggc gtggtcgtcg tcgaggcggc ggatggtgcc
    12001 gaagatcccg gcgaccgtct cgatctcgtc gcccacctgg agggagccga gaagctcgag
    12061 atactgcttc cggcgccgct gctgcggacg gatcatcagg aaatacatgg caccgaacag
    12121 cagagcgagg aagatcaagc tcgtgtactg cccggactcg ctgccgcttt gcgcgaagaa
    12181 attgatcacg acaaaccctt catgcacgaa gatcccaccc gggaaagtcc caggtgggcg
    12241 cgcgcaattc taggagcggc ccgtcggacc gtcaatgcgc ccgcgaccgg gcagtttacg
    12301 gggtcccgcg ccgtccggcg cgaagctcgt cccgtccggc cgggcagggg ccggggcggg
    12361 ctcagagcag cgtctgggcg gcagggcggt tcagcccgag atgccggtat gcggcttcgg
    12421 tggccacccg gccccggggc gtgcgtttga ggaatccgag ccggagcaga tagggctcgg
    12481 ccacgtcctc gatggtctcg ggctcctcgt ggaccgcagc cgcgatcgtc gcgaccccga
    12541 ccggccctcc gccgaacttc tcgaccacgg cccgcagcag cgcgatgtcc agacggtcga
    12601 gccccagctc gtcgacctcg aagaccgcca gcgcgtcgcg cgcgacgtcc ccggggatcc
    12661 gcccgtcggc gcgcacgtcc gcgaagtcgc ggacccgccg aagaagccgg ttggcgacgc
    12721 gaggcgttcc gcgcgcccgg ccggcgatct cggttgcgcc ttcttcgtcg agcgggatct
    12781 ccaggaggcc gccgctgcgg tgcaggatct cgccgagatc gggcgcgtcg tagtagtcca
    12841 gccgcgccgc gaatccgaag cggtcgcgca ggggcccggt gagcaggccg gtgcgcgtgg
    12901 tcgccccgat gagcgtgaag cgcggcaggt cgatgcggat ggaccgcgcc gccggaccct
    12961 tgccgatgac cacgtcgagc gtgaagtcct ccatcgcggc gtacaggatt tcctcgacct
    13021 gggttcggag ccggtggatc tcgtcgatga acagcacgtc gccgggctcg aggttggtca
    13081 cgatcgccgc caggtcgcct gcccgctcga tcgcgggacc gctggtcgtg cggatctgcg
    13141 agccgatctc cgaggcgaca atgtgggcga gcgaggtctt gccgagcccg ggcggcccgg
    13201 agaacagcag atgatccggc gcggctcctc gtgccagcgc cgcgtccagg atcaacgaca
    13261 gctgctcctt cacgcgcggc tggccgacaa actcctcgag ccgcttcggc cgcagcgccg
    13321 catcgaaatc gtgctcctcc ggcaggacgc tgccggtcag gatctcgctc atcgccgcgc
    13381 tcccagcgtc ttgagcgcca ggcgcagcag ctcctcgctt ccggacgcgg cggacccggc
    13441 gacggcctcg agcgcctcac gcgcctcggt ggtggagtag ccgagcgaca gcaaggcttc
    13501 ccgcgcttcc tcgaatgcgg gccgcgcatc ctccggcagc gaaacgcccg cgaaggacgc
    13561 ctctccgccg agcttttcct tcagctccag gatcatccgg ccggcagtct tcttgccgat
    13621 gcccggcacc agcgtcagcg cgtcgagatc ctcggcggcg acggcctttc gaagcgcgtc
    13681 tggcgagtag gccgacagga tcgcgagggc ggccttgggg ccgatgccgt tgacggcaat
    13741 cagcgtctcg aacacgtcgc gctcgtccga ggtgggaaaa ccgtagagcg tcatcgcgtc
    13801 ttcgcggacg tgcaggtgcg tgtgcaaggt cgccttcgac ccggtccgtc cgatcgccgc
    13861 gatcgacgag gccggacagt gcagccggta gcccaccccg cccacgtcta ggaccacgcc
    13921 ggtcgggccg gtcgcggcga ccacgccgct cagcatcgcg atcatcgcgc ggctccgcgc
    13981 agcgccgccg gagcggtcag ccacggacgt ccgtgcagcg ccgcgacggc aagcgcgagc
    14041 gcgtcggccg cgtcgggctc ggtgaggtcg gcggacagcg acaggatccg gcggatcatg
    14101 aacatgacct gcttcttgtc cgcgtccccg acgccgacga cggcgcgctt gacctccagc
    14161 ggcccgtact cgtagacggg caggccgcgt tcggcggcga cgcacatcgc cacgccgctc
    14221 gcctgcaagg tccgggcggc cgtgcgcacg ttgcggttga agaagatccg ctcgatcgcc
    14281 atcgcggccg ggccggtccg ctccatcgcc tcgagcagcc ggatgcgcaa ggtcgcgagc
    14341 tgctcggccg gggtttcgcc ggcggcgcgc agcgtgccgt gctcgaccga tcgaagacgg
    14401 ccgccgcgct cctcgacgat gccgtagccg gcacgcgtga gcccggggtc gaagccgatg
    14461 atccgcacag gcgctccccg ctcgaacata tgtacgaact ctaactggtg gcaaggacgg
    14521 gttcaaggac gcccggaacg cccgggtccc ggaaatcaca acgctgtgat tctactcgcg
    14581 gatcgggaac gccggcacga acaccacgac gacggcttcg ctccactccc ccacgttgcc
    14641 ggcgaagtcg gtagccctgg ccgaaaccgc ccacaccccc ggcggcacct ccccgtcgac
    14701 ctgccactgc cagaaggttc cggaggccgg cacgcgcacg atcttgggag gagcctccag
    14761 accgaacggc atcgaggccg gccgcgcgat gcgcacgtcc acccaggcga gcgcgatgtc
    14821 gtcggtcgcg cttccgcgga ccgagcctgg cttgcgcagc cccgcctgaa gctggttgcc
    14881 gcctacgaag gccgcaggag gctcggtgtc ggcggtgacg tggaaggtaa ccggcgccga
    14941 gtcggcggcg gaccgtccgt tcacatgagt cgcgcgggcg acgatcgtgt gcgtgccgcg
    15001 caggaagtcc agatcgatcg cccaccggcc gtcgtttccg gctagggcgc ggccgatcgg
    15061 cgcgccggcc gagcgaatct cggcgtgggt ctgcggctct gccgttccgg tgatccgcac
    15121 gatgcgcgtc gggatggtcg cgccgtcggc gggtgcggtg atcgccgccg gcgcgggagc
    15181 ggacgtgcgg tccgtgatcg ccccgcacat cccttggcag tcgagcgatc gtgcgtcgat
    15241 ccgggaatgg aactcgcagc gatacaaggc ggttccgccc gagaacgtgg tcgagaccac
    15301 gtcgccctgc tgcacgagcg agtgcgcgaa ggtggcgccg gcgtaagcgt ggatggtgtg
    15361 gttgcccgtc tcgacggtcc agcggatcaa gtccccgccg ggagcggtga cgacggcggg
    15421 atcgaacgcc gcgctgcgca cggt
//
LOCUS       DATLFO010000295         7971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_27978, whole genome shotgun sequence.
ACCESSION   DATLFO010000295 DATLFO010000000
VERSION     DATLFO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27978"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VM841_11915"
     CDS             complement(<1..480)
                     /locus_tag="VM841_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HVL90928.1"
                     /translation="MSQISPSAGYSLRLLVNVRSVPGTLGKLMTAIGRAGGDLGAVDL
                     IEHRGNVVVREITVKCRDDSHGRQIIKAAQRVQGVEIRGTSDRTFDIHAGGKIGVTPK
                     FPLRSRDDLSMAYTPGVGRVSSAIAENPERVYDYTIKANSVAVFTDGSAVLGLGNIGP
                     "
     gene            complement(510..1970)
                     /locus_tag="VM841_11920"
     CDS             complement(510..1970)
                     /locus_tag="VM841_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90929.1"
                     /translation="MKPTRTGPSRGRIMAAAALAVAAIVPLAPARALMPYQGWTPSSF
                     GMLPECGKYETSGYAVSGCLITRRMRFHPSRTGVAVAGTLRQGPWITTNGLTWTPLAP
                     HCLSTLYTVESAVGNGLTACGVEDIAFDPVDPQTIYVTAFNFSSPLLIGYNIEPAGVF
                     RSTDLGLTWQRISPPIRGAGLAVIRSGSRTTIMAGQIQHTDWDIGTTTSLIVSDDGGG
                     TWRKVTLPAARGCNRDVFLSSSYLVASIVPHPTNPNLIYAATNGGVYMSTNRGSSWSL
                     VLAKCISTPVDIGMIWGLTISPDGKTVFAGLWDGTIRMSSTTRTSTRTWKHVTTIDGT
                     MISHLMPDMRDPTGKTLYVAALNKAAGKTNTSAGVYRIKIGSPTTSTLLWDSWASGVN
                     HAMGSLPRGPYNLIKDSPAFWLAQNPVERDLIYLSQAAGGIFWRSEGPPSPSAQNPGL
                     PALPGVPAVPTSTPSAAIPTATGTPRVPYPTSTPTP"
     gene            2096..3115
                     /locus_tag="VM841_11925"
     CDS             2096..3115
                     /locus_tag="VM841_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HVL90930.1"
                     /translation="MSRPLLGHLDPDFLSLMDEVRTMLRQAFRTSNETTFAVSGTGSA
                     GMEAALVNLLEPGDVAVVGIAGVFGERMADVARRCGAQVREVRAEWGAPLEPAQVEPL
                     LEGAKVVSFVHAETSTGMRQPVEALSAMARDAGAMVVLDAVTSLAGLPVEVDAWGVDV
                     CYSGTQKCLSVPPGLAPVTFSPRAVEAIAARTTPVQSWYLDVSMLTRYWGSERVYHHT
                     APISMLFGLHEGLRLVLDEGLETRWARHGRLGAVLSKELDALGFEQFAREGFRLPQLQ
                     AVRLPEGRDADALRRALLDRFDVEVGGGLGPLAGNAWRIGLMGESCHEDAVEFLLEAI
                     REVLD"
     gene            complement(3112..5607)
                     /locus_tag="VM841_11930"
     CDS             complement(3112..5607)
                     /locus_tag="VM841_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90931.1"
                     /translation="MRLRFLVGALAAVLVIPGVRAAAETPPKALTVANDSTRPLSWDG
                     TAVLAPAGVRQQQLRDLSAGAGAAHQRFVAKIGPAREARVTIWIHWETPEDLSLWVTD
                     GKQAVYASSDEPAGTDEIASFDVVNGATYTVEVYAKAGAPTGVRPQYEATAWVRHADG
                     INATKRSKPALQFVKPNVCGDPNYRLVNGARVYQAPPPCYVKLKMPINIVFVGFDNNE
                     VQAHRPEVAGQFPDPDPRAQMVKPVVESESNTGGGLPLARNGGNQVRQAAMLQGSAMS
                     YLPWAYEFGDPNIVVTDEAWTRRLFAAAKAATTAGDYAHPWDRAYLESYNARAAALRR
                     DHPVVPGSPVDFIDAIKVEDWIAKNPPKGLKFGLNDSALGFTYFVIDTYRPSYAGQYF
                     NLDRYHHFKVMNSLTVDPDTGEQRGFDWGRVWGGRYRFLMLDVGAAPNSFEGSTPVNG
                     ATYRTAGGGDSALLDPPVWEYANCAAIDCRDLLPIMYQRIGENAVNALYLRFARGYAS
                     RPRGTDLFLLSSNTWTDANAVASGAPAYDAKLVAARVYDLMRYTRVVPFTRTKMLKAG
                     DPEQAALDAARLQSAARLPSGVGADPHPVMRMMLRDRAEYAPKVPGSISIPIVNVRLP
                     GATTWNGADADGSRLAHLGPDPWGLLHGINDATAAPAGRGFTASAIRNAGRVLGLYPA
                     TEGVAYTRDLPPDVNAPRPHPAKADEQYRAYYKTFDWTHATTATPMAHGWFYSRFEVL
                     DKDAIGLAHAFDWLDRSLDDVADAFAILDVRGHKTLPKAVEDQILKAGSFMGATVGYA
                     QFGQMASSVRSARRAKAETEKVVAFAIRAGARR"
     gene            5836..7065
                     /locus_tag="VM841_11935"
     CDS             5836..7065
                     /locus_tag="VM841_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVL90932.1"
                     /translation="MRSVAAVCALLLGLTACSSDSPAAGPTPGESPTPTPTSTPTSTP
                     TASPSPTRTEPAATLSNLSIRLRTVASGLDNPVGVTHAGGEPLYVIEQAGRIMRVATG
                     ASPQAYLDIRGRIASGGERGLLGLAFHPSFASNGRLFVNYTDTGGSTRISEFRGNPDG
                     ADAGSERVLLTIDQPFANHNGGAVVFDKTGMLLIATGDGGSGGDPRNNGQNLSTLLGK
                     ILRIDVDRGSPYGIPPDNPFRNRSGARAEILDYGLRNPWRISIDRETGDLWIGDVGQN
                     RLEEIDVHAAPLNSAKNFGWRIMEGRSCYNASSCNRSGLTQPVAQYDRSQGDRSVTGG
                     YVYRGSASPKLRGFYFYADAYSGFIRALSAADARDGSADSRIVSRTRKPIVSFGETAA
                     GEVLVVTHDGLIMQITS"
     gene            7163..7582
                     /locus_tag="VM841_11940"
     CDS             7163..7582
                     /locus_tag="VM841_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HVL90933.1"
                     /translation="MKPASDRARRRVAEGRVARLGTINPDGTPNLIPFCYVLDNDTIY
                     TAVDRKPKTTTSLRRIDNLLARPDCMVIVDHYEEAWENVWWVRIRGRGRILDGGDEFG
                     HADRLLRMKYPQLRDDPLPPAIIAIDAVAWSEWSYTP"
     gene            complement(7708..>7971)
                     /locus_tag="VM841_11945"
     CDS             complement(7708..>7971)
                     /locus_tag="VM841_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HVL90934.1"
                     /translation="VWCFVVLKPGTDATGELADTLRGMVADGLGKAFRPDAVRFVSAL
                     PKTRSAKILRRAVRAAALGEDAGDLSSLENPAALDEIKATRVE"
BASE COUNT         1216 a         2655 c         2840 g         1260 t
ORIGIN      
        1 gggtccgatg ttgcccaggc ccagcacggc gctgccgtcg gtgaagaccg cgaccgagtt
       61 cgccttgatg gtgtagtcgt agacgcgctc ggggttttcg gcgatggcgc tcgagacgcg
      121 tccgacgccg ggcgtgtagg ccatcgacag gtcgtcgcga ctgcgcagcg gaaacttcgg
      181 cgtgacgccg atcttgccgc cggcgtggat gtcgaaggtg cggtccgagg tgccgcgaat
      241 ctcgacgcct tgcacgcgct gcgcggcctt gatgatctgg cggccgtgcg agtcgtcgcg
      301 gcatttgacg gtgatctcgc gcacgacgac gttgccgcga tgctcgatca gatcgaccgc
      361 gccaaggtct cctcctgcgc ggccgatcgc ggtcatcagc tttccgagcg tgccgggcac
      421 gctgcgaacg ttgaccaaca gacgaagtga atacccggcg gacggcgaga tctggctcat
      481 atccgcgcga ttatagagcg cgcgtcatgc tacggcgtcg gcgtggacgt cgggtaagga
      541 acgcgcggcg tcccggtcgc ggtcggaatc gcagcgctcg gtgtggaggt gggcaccgcg
      601 gggacccccg gcagcgccgg aaggccaggg ttctgcgccg acggcgacgg cgggccctcg
      661 cttcgccaga agatgcctcc ggccgcctgc gacaggtaga tcaggtcgcg ctcgaccggg
      721 ttctgcgcga gccagaaggc cggcgagtcc ttgatcaggt tgtacgggcc gcgcggcagc
      781 gatcccatcg cgtggttgac cccgctcgcc catgaatccc acagcagcgt ggacgtggtc
      841 ggcgagccga tcttgatccg gtacacgccc gcggaggtgt tcgtcttgcc ggcggccttg
      901 ttgagcgcgg cgacgtagag cgtcttgccg gtcgggtcgc gcatgtccgg catcaggtgc
      961 gagatcatcg tcccgtcgat ggtggtcacg tgcttccagg ttcgcgtcga ggtccgggtg
     1021 gtcgaggaca tgcggatcgt tccgtcccac agacccgcga agacggtctt gccatccggc
     1081 gagatcgtga gaccccagat catgccgatg tccaccgggg tggagatgca cttggccagc
     1141 acgagcgacc acgacgagcc ccgattggtg ctcatgtaga cgccgccgtt ggtcgcggcg
     1201 tagatcaggt tcgggttcgt cggatgcggc acgatggatg cgaccagata cgacgaggac
     1261 agaaagacgt cccggttgca gccgcgagcg gcgggcaagg tgactttgcg ccaggtgccg
     1321 ccgccgtcgt cggagacgat caggctcgtg gtggtgccga tgtcccagtc cgtgtgctgg
     1381 atctgcccgg ccatgatcgt ggtgcgcgat ccggaccgga tcaccgcgag cccggcgccg
     1441 cgaatgggcg gcgagatgcg ctgccaggtc agcccgagat ccgtcgaccg gaacacgccg
     1501 gccggctcga tattgtagcc gatcagcagc ggcgaggaga agttgaacgc ggtcacgtag
     1561 atggtctgag gatcgaccgg gtcgaaggcg atgtcctcga ccccgcaggc ggtcaagccg
     1621 ttgccgacgg cgctttcgac agtgtagagc gtggacaggc agtgaggcgc gagcggcgtc
     1681 caggtcaatc cgttggtggt gatccacggc ccctgccgga gcgtgcctgc gaccgcgacc
     1741 ccggtcctcg acgggtgaaa gcgcatgcgc cgcgtgatca ggcatcccga cacggcgtag
     1801 ccgctcgtct cgtacttgcc gcattcgggc agcatgccga acgacgacgg cgtccagcct
     1861 tggtagggca tcagcgcgcg cgcgggagcg agcggaacga tcgcggcgac ggccagcgcg
     1921 gcggccgcca tgatccgtcc gcgcgacggc ccggtgcgag tcggcttcac gatctataaa
     1981 cgaccagcac ccgcagatgg gatcaaggtt atgcttccgg ctcgtgcgga actaccgaat
     2041 tcgacgtcta ttcggacccg gtccctcccc cgtggcgccc tcggtgctgc aagcgatgag
     2101 ccggccgctg ctcgggcatc tcgatccgga cttcctgtcc ctgatggacg aggtacgaac
     2161 gatgctgcgc caagcgttcc ggacctcgaa cgagaccacg ttcgcggtca gcggcaccgg
     2221 cagcgccggc atggaggccg cgctggtcaa cctgctcgag cccggcgacg tggcggtggt
     2281 ggggatcgcg ggtgtgttcg gcgagcgcat ggccgacgtg gcgcgtcgct gcggcgcgca
     2341 ggttcgcgag gttcgcgccg aatggggtgc gccgctggag ccggcgcagg tcgagccgct
     2401 cctggagggc gcgaaggtcg tgtcgttcgt gcacgcggaa acatcgaccg gcatgcggca
     2461 gccggtcgaa gcgctgtcgg cgatggcccg cgacgcgggc gcgatggtcg tgctcgatgc
     2521 ggtcacctcg ctggccggac tcccggtcga ggtggacgcc tggggcgtgg acgtgtgcta
     2581 ctcgggcacg caaaagtgcc tgtcggttcc gccgggcttg gccccggtca ccttctcgcc
     2641 gcgggccgtc gaggcgatcg cggcgcgcac gacgccggtg cagtcgtggt atctggacgt
     2701 gtcgatgctg acccggtact ggggctcgga gcgcgtctac caccacaccg ccccgatctc
     2761 gatgctgttc ggcctgcacg aaggccttcg gctggtgctc gacgaagggc tggagacgcg
     2821 ctgggcgcgg cacgggcgac tcggcgcggt cctttcgaag gagctcgatg cgctcggctt
     2881 cgagcagttc gcgcgcgagg gcttccgtct gccgcagctg caagcggtgc gcctgcccga
     2941 aggacgcgac gccgacgcgc tgcgacgcgc gctgctggac cgcttcgacg tcgaggtcgg
     3001 cggcggcctg gggccgctgg ccggaaacgc gtggcgcatc gggctcatgg gcgagtcctg
     3061 ccacgaggac gccgtcgagt tcctgctcga agcgatccgc gaagtgctgg attagcgccg
     3121 cgcgcccgcg cggatcgcga acgcgacgac cttctcggtc tcggccttcg cgcggcgggc
     3181 actgcgcacc gacgacgcca tctggccgaa ctgcgcgtag ccgacggtgg cgcccatgaa
     3241 cgagccggct ttcaggatct gatcctcgac ggccttgggc agcgtcttgt ggccgcgcac
     3301 gtccaggatc gcgaacgcgt cagcgacgtc gtccagcgac cggtcgagcc agtcgaaggc
     3361 gtgcgccaag ccgatcgcgt ccttgtcgag cacctcgaaa cgcgagtaga accagccgtg
     3421 cgccatcggc gtggcggtcg tggcgtgcgt ccagtcgaag gtcttgtagt aggcgcggta
     3481 ttgctcgtcg gccttggcgg gatgcgggcg cggcgcgttg acgtcgggcg gcaggtcccg
     3541 cgtgtaggcg acaccttcgg tcgcgggata cagcccgagc acgcggccgg cgtttcggat
     3601 cgccgaggcg gtgaagccgc gcccggccgg cgccgcggtc gcgtcattga tgccgtgcaa
     3661 cagcccccag gggtcgggac ccagatgcgc gagccgcgag ccgtccgcgt ccgcgccgtt
     3721 ccaggtggtt gcgccgggca aacgcacgtt gacgatcggg atcgagatcg agccgggaac
     3781 cttcggcgcg tactcggcgc gatcgcgcag catcatgcgc atcaccggat gcggatcggc
     3841 gccgacgccc gacggcagcc gcgccgccga ctgcagccgc gccgcgtcca gcgccgcctg
     3901 ctcgggatcg ccggccttga gcatcttggt gcgcgtgaac ggcacgacgc gcgtatagcg
     3961 catcaggtcg tagacgcgcg ccgcgaccag cttcgcgtcg taggcgggtg cgccggaggc
     4021 gacggcgttt gcatccgtcc aggtgttcga cgacagaagg aaaaggtcgg tgccgcgcgg
     4081 ccgcgaggcg tagccccgcg cgaagcgaag gtagagcgcg ttgacggcgt tctcgccgat
     4141 ccgctggtac atgatcggaa gcaggtcgcg gcagtcgata gcggcgcagt tcgcgtactc
     4201 ccacaccggc gggtccagga gcgccgagtc tccccctccc gcggtccggt aggtcgcgcc
     4261 gttgaccggg gtggagcctt cgaacgagtt gggcgccgca ccgacgtcga gcatcaggaa
     4321 ccggtagcgg ccgccccaga cgcggcccca gtcgaagccc cgctgctcgc cggtgtcggg
     4381 atccacggtc agcgagttca tcaccttgaa gtggtggtag cgatccaggt tgaagtattg
     4441 gccggcgtac gaaggccggt aggtgtcgat cacgaaatag gtgaagccca gcgccgaatc
     4501 gttcagaccg aacttcaggc ccttcggcgg gttcttggcg atccagtcct cgaccttgat
     4561 cgcgtcgatg aaatcgaccg gcgagccggg cacgaccgga tggtcgcgcc gaagcgcggc
     4621 ggcgcgcgcg ttgtaggact cgagatatgc gcggtcccag ggatgcgcgt agtcgcccgc
     4681 ggtcgtggcg gccttcgcgg ccgcgaacag gcggcgggtc caggcctcgt cggtgacgac
     4741 gatattggga tcgccgaact cgtaggccca gggcaggtag ctcatcgccg agccttgcag
     4801 catcgccgcc tggcggacct ggttgccgcc gttgcgtgcc agcggcaatc ccccgccggt
     4861 gttcgattcg ctttcgacga cgggcttgac catctgcgcg cgcggatcgg gatcggggaa
     4921 ctggccggcg acctcgggcc ggtgcgcctg gacctcgttg ttgtcgaagc cgacgaacac
     4981 gatgttgatc ggcatcttca gcttcacgta gcaaggcggc ggcgcctggt agacgcgggc
     5041 accgttgacg agccgatagt ttgggtcgcc gcacacgttc ggctttacga actggagcgc
     5101 gggcttggat cgcttggtcg cgttgatgcc gtcggcatgc cggacccagg cggtcgcctc
     5161 gtactgggga cgcacgccgg tgggggcgcc tgccttggcg tagacctcga cggtgtaggt
     5221 cgcgccgttg accacgtcga acgaagcgat ctcatccgtt ccggcgggct cgtcggacga
     5281 ggcgtagacg gcctgcttgc cgtcggtgac ccacagcgaa aggtcttcgg gggtttccca
     5341 gtggatccag atcgtgacgc gcgcttcgcg cgcggggccg atcttggcga cgaaacgctg
     5401 atgcgccgcg cccgcgccgg cggaaaggtc gcgcagctgc tgctgacgca cccccgccgg
     5461 agccagcacc gcggtgccgt cccacgacaa cggacgggtc gagtcgttgg cgaccgtcag
     5521 ggctttgggt ggggtttcgg cggcggcgcg gacacccggg atcacgagaa ccgcggcgag
     5581 cgcgccgacg aggaaccgca atcgcattcg ggtcatattt ccctaggaca ctatttcctt
     5641 agtaaaccat ctgtccggtt cgcccccccg cgggacggcc ggccggagcg tcccctcgat
     5701 cgctccggac ggcacgggtc ccgcgtgttc gtcttctcaa cgattcgggc gcgcgcttgg
     5761 acgttccttc gtaagcggag aaaccgatac atccgacccc gggcggcccg gtgaccgagc
     5821 cgctaggctc acgccatgcg gagtgttgcc gccgtctgcg cgttgctgct gggcctgacc
     5881 gcctgttcgt ccgattcgcc cgccgccggc cccacccccg gcgaatcacc gacgcccacg
     5941 cccacgtcga cgcccacgtc cacgcccacg gcgagcccgt ccccgacgcg cacggaaccg
     6001 gccgcgacgc tgtcgaattt gagcatccgg ctgcggaccg tcgcgagcgg tctggacaac
     6061 ccggtcggcg tcacgcacgc gggcggcgag ccgctgtacg tgatcgagca ggccggccgg
     6121 atcatgcgcg tcgcgacggg ggcgagcccg caggcgtatc tcgacatccg cggccgcatc
     6181 gcatcgggcg gcgagcgcgg cctgctcggc cttgccttcc acccgagctt cgcctccaac
     6241 ggccggctgt tcgtcaacta caccgacacg ggcggcagca cgcggatcag cgagttccgc
     6301 ggcaacccgg acggcgccga cgcgggatcc gaacgcgtcc tgctgacgat cgaccagccg
     6361 ttcgcgaatc acaacggcgg cgcggtcgtg ttcgacaaga ccggcatgct gctgatcgcg
     6421 accggcgacg gcggcagcgg cggcgacccg cgcaacaacg gccagaacct ctcgacgctg
     6481 ctgggcaaga tccttcgcat cgacgtcgat cgcggctcgc cctacggaat cccgcccgac
     6541 aatccgttcc gaaaccgctc gggcgcgcgc gccgagatcc tcgactacgg cctgcgcaac
     6601 ccgtggcgga tctcgatcga ccgcgaaacc ggcgatctgt ggatcggcga cgtcggacag
     6661 aaccggctcg aggaaatcga cgtccatgcc gcgccgctca acagcgcgaa gaacttcggg
     6721 tggcgcatca tggagggccg ctcgtgctac aacgcgtcgt cgtgcaaccg cagcggcctg
     6781 acccaaccgg tcgcgcaata cgatcgctcg caaggcgacc gctcggtgac cggcgggtac
     6841 gtatatcgcg ggagcgcgtc gccgaagctg cggggcttct acttctacgc cgacgcctac
     6901 agcggcttca tccgcgcgct gtcggcggcc gatgcgcgcg acggctcggc cgatagccgg
     6961 atcgtgtcgc ggacccgcaa gccgatcgtg tccttcggcg agaccgccgc cggcgaggtg
     7021 ctcgtggtca cgcacgacgg cctgatcatg cagatcacga gctgatggat cccggattcg
     7081 ggatggtact ggtcatcgcc gccgggctcg ccgcctcgct gctgtcgctg tggctgcggt
     7141 ccaagcccag aaagcccaag cgatgaagcc ggcgtcggat cgcgcacgcc gccgcgtcgc
     7201 cgaggggcgc gtcgcgcggc tgggcacgat caaccccgac gggacgccga acctgatccc
     7261 cttctgctac gtgctcgaca acgacacgat ctatacggcg gtcgatcgaa agcccaagac
     7321 cacgacgagc cttcgccgga tcgacaacct cctcgcccgg ccggactgca tggtgatcgt
     7381 ggaccactac gaagaggcat gggagaacgt gtggtgggtc cggatccgcg gacggggccg
     7441 catcctggac ggcggcgacg agttcggcca cgccgaccgg ctgctgcgca tgaagtaccc
     7501 acagttgcgc gacgatccgc tgccgcccgc gatcatcgcg atcgacgccg tcgcctggag
     7561 cgaatggtcc tacacgccgt aggcggctac accagctcga ggtcggtctt ggcgaagtcc
     7621 ttgcccgtgc atagcaggcg ccgatcgcgg cgggctctcg catcatcagg tcggccagct
     7681 cttcgaagcg cggctcgcgg ccgaagatta ttccaccctg gtagctttga tctcgtcgag
     7741 ggcggcgggg ttttcgaggc tggacaggtc tccggcgtct tcgcccagtg cggcggcacg
     7801 cacggcgcgg cgaaggatct tggcgctgcg cgtcttcggc agcgcgctca cgaaccgcac
     7861 ggcgtccggc cggaaggcct tgccgagccc gtcggcgacc atgccgcgaa gcgtgtcggc
     7921 cagctcgccg gtggcgtcgg tgccgggctt gagcaccacg aagcaccaga c
//
LOCUS       DATLFO010000296        27295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_76973, whole genome shotgun sequence.
ACCESSION   DATLFO010000296 DATLFO010000000
VERSION     DATLFO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27295
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76973"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VM841_11950"
     CDS             complement(<1..201)
                     /locus_tag="VM841_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL90935.1"
                     /translation="METCEFAVVGAGAMGAAAAWSLSRAGRSDVVVLDRFDLDHDRGS
                     SHGPMRIFRFAYDDPFSVRLAQR"
     gene            410..1600
                     /locus_tag="VM841_11955"
     CDS             410..1600
                     /locus_tag="VM841_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVL90936.1"
                     /translation="MDFRDTPEEASFRKEVRAWFQANLPEGWGSTSLYMESPDKRIEF
                     LRDFQRKLYEGGWAGLSWPKEYGGRGATLIEQVIFSQEAARAKAPQPLGVIGLGMAGP
                     TIIAHGTDEQKRRYLAKILSGEEIWCQGFSEPGSGSDLASLKATAVLDGDAYVVNGQK
                     VWTSYAHIADWCILLARTDNTGPKHSGITYFLMDMHAPGVEVRPLRQITGDAQFNELF
                     LTDVRIPKENILGEINDGWRIAMTTLLHERGTLGFALAVAGGVALDELVAFAKTAPRA
                     GGGVAYDDPVVRQRIAKLHEEVQANLLNNYRALATMMKTGIPGPEGSLSKLMFSETNQ
                     ELGKLAVEIAGGYGLLEAGERPDVARWQYAHLRARGNSIEAGTSEILRNIIAERVLGL
                     PKHK"
     gene            1616..2713
                     /locus_tag="VM841_11960"
     CDS             1616..2713
                     /locus_tag="VM841_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL90937.1"
                     /translation="MEFGLSEDQQTLKKSAREFLEGEAGIALARRMLEHESGFEPSLW
                     THMAELGWMGIAIPESEGGLGLGMLELSLLLEEMGRVALPSPYFSTVALAVPVVASLA
                     DAATRSEVLGAVAAGERKLTLAYAEASGAFDPAAIEAAAQGSGDLWTVTGTKMFVPDA
                     HLADQIVVLARTGVGVGAFLVQAADCEISQNESLDRTRRLGTVTLGGVAARPLGGEDA
                     WAEIERALDRAAVGLAAEATGVADAVMSMSVAYAKERQQFGRAIGSFQAVSHTCADML
                     VAAESARSTTYYAAWAIDEGAPDAPRAAATAKAAATTGAMRVCEDGIQVHGGIGFTWE
                     HDMHLWYRRAKLCELLLGPATVWRERIASLL"
     gene            2716..3405
                     /gene="nth"
                     /locus_tag="VM841_11965"
     CDS             2716..3405
                     /gene="nth"
                     /locus_tag="VM841_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVL90938.1"
                     /translation="MGKPKGEPRAAWVEDETPPEKKKRAAAIRTRLAKEYPDARCALN
                     FSNPLEILVATVLSAQCTDAKVNQVTETLFEKYRTAEDYLAAKPGELEKDIHATGFFN
                     QKAKSIRGLSRLIVEEHGGEVPRTMEEILRLPGVARKTGNVVLGNAYGVVEGIAVDTH
                     VHRLAWRLGLSDQDDPVKVERDLTALFPKRRWLTLTYLLIEHGRNVCFARKPRCDECV
                     VASRCPARRMP"
     gene            3452..4000
                     /locus_tag="VM841_11970"
     CDS             3452..4000
                     /locus_tag="VM841_11970"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVL90939.1"
                     /translation="MPRIYTKTGDDGTTGLLYGGRVSKDDIRTEAYGTSDEAVAALGV
                     ARAQTADVTLAETLVRLQRELFVVGAELATGEGAWDKLKPGESIVDEPMVEALEELID
                     EFVAKTRMPEEFVVPGNTSVSAAIDLARAIVRRCEREVTTMTNEGLLPDRECLRYLNR
                     LSDLLFVLARYEEAGFTPLHPA"
     gene            4031..4789
                     /locus_tag="VM841_11975"
     CDS             4031..4789
                     /locus_tag="VM841_11975"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HVL90940.1"
                     /translation="MNSSTMPKTVLMLTAAAVLLGACATKQTQPEGGGSGAGPGVAVQ
                     APESVKGNVVTLDLTAKNFEIVKADGDTSGKTGHFHIFIDREPVAAGEVIAREAGIVH
                     STDAKTKIMGLAPGEHTFIVVLGDGNHARVGDAQAKATVKVEGPSVDASAPATVKAGE
                     AVTVSMKIDGATLVGADGDTSGKTGHLHIFIDRDPILEGPIPVEDGIVHTKETSHTIE
                     GLAAGDHTIWVVLGDGTHTSFADPVMDKVTVTVA"
     gene            complement(4846..5634)
                     /gene="panB"
                     /locus_tag="VM841_11980"
     CDS             complement(4846..5634)
                     /gene="panB"
                     /locus_tag="VM841_11980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVL90941.1"
                     /translation="MSVTTHDLAAFKARGEKFAMLTAYDYPTAQILDQAGVPVLLVGD
                     SLANTILGYDTTLPVTMDEMIHHCKAVARGSRNALIVGDMPFMSYQASDEDGLRNAGR
                     FLKEAGAHAVKIEGPRIELAAKLAERGIPVMGHLGLTPQSVHLFGGYKVQGRTEEQAA
                     SILRWAKELEDAGCFAIVLEVVPAALGASVTKALSIPTVGIGAGPDTDAQVLVINDLA
                     GINDWAPKFVRKYADVRGVIGDAVTRWMSDVKDGRYPDDETSYT"
     gene            complement(6239..7594)
                     /locus_tag="VM841_11985"
     CDS             complement(6239..7594)
                     /locus_tag="VM841_11985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL90942.1"
                     /translation="MSDGVLRPGEMLGEPAALVRPRRLPRKRTVDALAVPRLQRRLVG
                     AIFATTVAGVIALKVFTLRGIEREIFWGSYAILVGVYLLSRFALAARYRPTRAVVVDD
                     ALPAVSIVVPAKNEEAAIEQTLRRALASAYPADRLEIIAVDDGSTDGTLPAMRAVQRD
                     HPDRLRIVSMENRGKRFAMAEGFVRSRGDVVVFVDSDSFLDPESVRRMMDYFADERVG
                     CVAGHADVANASQNVLTRMQSVRYYVAFKVVKASEALFGTVTCASGCFSGYRRAAVEP
                     VLHEWLNQTFADTASTYGDDRSLTNHVLINKWKTLYAPDATASTLVPETLSQFMRQQL
                     RWKKSWIRESLRAGRFMWKRNVLAAWAFYTGVLLTLMAPVVFLRYVVVRPAMNAGHPW
                     IYATGIVLMAVLFGLYHRMRRRSSDWLFGILYALFYSTVMIWQTPYALATLRDGKWGT
                     R"
     gene            complement(7591..8910)
                     /locus_tag="VM841_11990"
     CDS             complement(7591..8910)
                     /locus_tag="VM841_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HVL90943.1"
                     /translation="MRVESRMLFNAATHPAAAAHVDANIVAIVGLGYVGLPTALAYAG
                     RGRGVIGIDTSEPRLRRIRTGEVDLLDGDRSRLARALSDPSFRLTADPTLLRCAGTVI
                     VCVPTPVDEHMVPDLEPLKRACATVVRNASPGQTIVLTSTSYVGTTRELLIEPLANEG
                     LDAGRDVFLAFSPERVDPGNGRFPQDAVPRVVGGATAECAERAARMIGAIAPHIHIVS
                     SPEAAEMTKLYENTFRAINIAFANEMAGAASALGVRIDEVIDAAATKPYGFMRFTPGP
                     GVGGHCIPCDPRYLQWQMQARRTPMPLLDRTMASIAERPAAIVTRIRDVLSDRGTGLR
                     DARVLVAGVAYKPGVEDVRESPAGEIIASLRSRGAIVEYHDPMVPVFTDALGVKMRSV
                     AVLQPETYDLILAHTIHPGFDAALLRSAPVLLDATYRLPGWPNRVLP"
     gene            complement(9040..9408)
                     /locus_tag="VM841_11995"
     CDS             complement(9040..9408)
                     /locus_tag="VM841_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL90944.1"
                     /translation="MKVDELMSTDLCTVDPEATLAEASTLMGERGVGSAMVIDEGRLV
                     GIVTERDVVRAMSNAHDAPVRPVAEWMTRKPGTATPGTSVKEALQLMVDGGFRHLPIC
                     DGDQPVGMVSMRDIARALAG"
     gene            complement(9405..11147)
                     /locus_tag="VM841_12000"
     CDS             complement(9405..11147)
                     /locus_tag="VM841_12000"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HVL90945.1"
                     /translation="MRTLRLALAQINPTVGDLRGNALAVRSWLAKAEAAGADLVAFPE
                     LTLTGYPPEDLLLKRSFVDENLAVLARLAKDVGDAAAIVGFVDRDDGGLYNAAALLAR
                     GKVRGVYRKVRLPNYGVFDEKRYFVAGSAFPLFRVRGVQIGISICEDAWAGDGPVRSL
                     ARAGAEVVVNVNASPYHRGKGLERTILFRQRARDNGIFFAYLQTVGGQDELIFDGETL
                     VFDPNGHLLARGAQFAEDLVVCDLSLPAAPRKTASKAPVTDVSKPLARSRKRPAVPPR
                     SIPEPMGPEEEVYAALTLGVRDYLRKNGFAQAVIGLSGGIDSSLVAAIAADAIGAGNV
                     LGVSMPSRVTSTASIEDAKELASNLGIGFRVVPIDALVTGYEASLLDALGEPLPGAAL
                     DNVQARIRGTLLMGISNATGAIVLTTGNKSEMATGYATLYGDMAGGFAVIKDVPKTLV
                     YTLCRWRNERGPAAIPESVIVKPPTAELHEGQLDTDSLPPYDELDPILEAYVERDAGL
                     DEIAKAGFDEPTVGRVARMVDTAEYKRRQAPPGIKITARAFGRDRRLPITNGYREWAM
                     KIPGRKPGRGKDPR"
     gene            11210..12541
                     /locus_tag="VM841_12005"
     CDS             11210..12541
                     /locus_tag="VM841_12005"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVL90946.1"
                     /translation="MDAAREQIDYVLRTVEERDIRFIRLWFTDVLGFLKAFAITPAEL
                     EDAFEEGMSFDGSAIEGYARVQESDMLARPDPSTFQALPQAKGDLEARMFCDITTPDG
                     APFPGDPRAVLRRNLERASAKGFTFYVHPEMEFFLFKDSKTPVPLDQGSYFDLTPLDS
                     AQDFRRRAIQALEAMGISVEYSHHEVAPSQHEIDLRFADALTMADNIMTYRLVVKEVA
                     QQMGLHATFMPKPLQGEWGSGMHTHVSLFEGDRNAFFDARTAGHLSKVGRAFVAGVLR
                     HAREITAVTNQWVNSYKRLIPGYEAPAYVCWSRHLRGALVRVPGAKPTKASSARIEIR
                     SPDPACNPYLTLSVILAAGLRGLDEGYELADEVVDDVDTMSEEERAKRGIVPLPQDLN
                     EAVDELERSELVADALGEHVFEQFIRNKRAEWDTFKAYVSPYERERYLPLL"
     gene            12578..15511
                     /locus_tag="VM841_12010"
     CDS             12578..15511
                     /locus_tag="VM841_12010"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HVL90947.1"
                     /translation="MVEPMAEPTSLSEMHAALARAGFARPVDAARHLQSVALGPKGMD
                     RVVAAAADAPDPDLAIAQICRFIAMTGRVPSAAVLQRLAAVLGLSTSLGAFLARHPDA
                     ADMLREDALAAPRTVATLRARARKLVATDDDPLTALRLFKRRELLRIAARDLAGGAAV
                     EEIAAELAALAEATLQGALDVLVAQNTPPPGTRFTVIGMGKLGGSELNYASDIDVVFV
                     FDSPDEAEAGPWANRIAEGLIKNLAATTEEGQAFRVDANLRPEGRDGALARSLTAFKA
                     YWERWAKPWEFQALIKARPVAGDEVLGAEFCAAAADVVYPERLAPEAVREIRAMKARA
                     ERVIAQRGLGNREIKRAPGGIRDIEFAVQLLQLVHGRRDASLRGGGTLPGLAALSRGA
                     YVGDDDAGELASAYVFLRRIEHRLQLAQERQTHTVPEDETRRRTLARSMGFRDGGTET
                     ALEAFDAAWRDIQAVVRRIHEKLFYRPLLERFAQAPALAPDAAQERLEALGFRSPGRA
                     LKFLGDLTAGLSRRSQLMRTLLPVMLDWMSDSPDPDLAVSNFRDVALALGGNPAALAA
                     LRDSPPVVELLCRVLGTSRLLAEHLLHTPELVPALAEQKALARKGRDKLMREAAEHIE
                     WRGDPDARDAAVRRFKRREMLRIACRDLAGGVSPDDIGRELSHLAEAALEAALGSLHE
                     EHPPPAGARFAVIGMGKLGGEELNYASDIDVMFVYDAPDEKGAHKWATMLAEGLLQRL
                     AATTEEGQAFPVDAQLRPEGKDGPLARSLTAYRAYYERWAKTWEFQALVKARPVAGDP
                     MLGQQFVDLIRPFVYPERLAPEKVRDIRSMKARIERERLGPRQDPATQMKVGRGGTID
                     IEFTVQLLQLMHGAHHPSIRVQGTVPAISAAAERQLLTIEQAKWLLEAYRFVNRTRNT
                     LYLIRGRAMDALPAKPEEAEVLARALGYPGPGARVQFGEDYRRVTRRARQVCEDVFYG
                     KRPTR"
     gene            15542..16429
                     /locus_tag="VM841_12015"
     CDS             15542..16429
                     /locus_tag="VM841_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL90948.1"
                     /translation="MSDRHVDVEGRRIYIREHGNGAPLVLLHGFPQTGEAWKQVADKL
                     SGRNRVFVPDLPGFGRSDAAVASDMQTVSRTLVRALAAAGAEKFALVGHDWGGSIALR
                     IALDHPQAVEKLVVVNAPFRSLDLTRGWHFLAFNVPLIPEAAMVLFGKRLVPMLIKAG
                     SKNKQAFDAETMKGYGEAFSDLGHARSTLAYYRTITRSTIMRGVRGALDKLPVPMPGG
                     RGTHDNEAGPRRVQVPTLIVWGERDPVLPPSLIPTIERDVDDVTVVRLPDIGHFVPEE
                     APSELAHEIDRFLNPHRGE"
     gene            16505..16831
                     /locus_tag="VM841_12020"
     CDS             16505..16831
                     /locus_tag="VM841_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL90949.1"
                     /translation="MDLVRSARGRPDETRRRMLDPMFADDPGQRAWLEGTLLRPGQVA
                     LLLQVSRRSVAAWAREGRLPSITTPGGHRRFRADDVRRLVDSLTVTVPAGSTPDRLAK
                     NQTVSE"
     gene            16956..17153
                     /locus_tag="VM841_12025"
     CDS             16956..17153
                     /locus_tag="VM841_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90950.1"
                     /translation="MKKIFAAAVVAAGLFASLPASADVIDIAGTIYVDDSDATVWQES
                     NGVEGLQREADGETPADSQLL"
     gene            complement(17224..17886)
                     /locus_tag="VM841_12030"
     CDS             complement(17224..17886)
                     /locus_tag="VM841_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90951.1"
                     /translation="MTTDRTPGSGREPDWDRAAERARQKARVPHRTEHDPIDGVVAVF
                     RSAAEAERAAAAVTAAVPGVTVRVGDDASHVAALRAEMAEEDARSFAGPSVGLYTPEM
                     AKGAAWGTAVWAVVGAILAAPFSLIPMGDLVIAVKLMIVTIVGAVAGGAFGFVAGGGA
                     AAKSPAEPMAAEAGVSLGVAVAGPDERKQVVRVLSAAEPVRLDEVRRGRPEATVERRR
                     HA"
     gene            complement(17999..19360)
                     /gene="glnA"
                     /locus_tag="VM841_12035"
     CDS             complement(17999..19360)
                     /gene="glnA"
                     /locus_tag="VM841_12035"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HVL90952.1"
                     /translation="MVDLRFTDLPGVTQHFSVPISELDERSFENGFGFDGSSIRGFQA
                     IQESDMLLIPDPTTAKMDPFLAHPTLFMYCFIKDPITGESYSRDPRNIAKKAEDHLKA
                     SGIADVSYWGPEAEFYIFDEARFDQNQHEGYYHLDSTEGAWGSGNPGARGYRPRYKEG
                     YFPVPPMDQYQDLRTEMVLEMQKLGIHIEVQHHEVGTAGQAEIDMRYGELLQTADRVM
                     DYKYAVKNVAWRNGKTATFMPKPLFMDNGSGMHTHQSLWRNGENLFYDETGYAMLSDM
                     ARYYIGGLLQHAPALLAFTNPTTNSYRRLVPGYEAPIYLVYSSRNRSACVRIPTYSNA
                     PAARRIEFRTPDPSCNPYLAFAACLQAGLDGVRNKIEPPEPMDKDLYDLAPEDKAQVQ
                     SVPGSLEEVLDALEADHDFLLEGGVFTEDVIETWINYKRLHEVDAMRLRPHPHEFALY
                     FDI"
     gene            19609..19836
                     /locus_tag="VM841_12040"
     CDS             19609..19836
                     /locus_tag="VM841_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HVL90953.1"
                     /translation="MRTTIDLDATVLAELKRRAKRESKSLGQLASELLAAALKQQSPG
                     KRSAIRFHTAAMGARVDLEDKDALWRTLEDR"
     gene            19833..20273
                     /locus_tag="VM841_12045"
     CDS             19833..20273
                     /locus_tag="VM841_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HVL90954.1"
                     /translation="MSSAIDANLLLYASDRSSPFHERALETVTMLAAGPALLYLFWPV
                     AMAYLRIATHPGVFARPLPWRAASENIRGLLERDNVRSPGEDEGFWPLFADTADAAGV
                     RGSLVPDAHLVALMRRSGVTTLWTHDRDFRRFDGITLQDPFAGA"
     gene            complement(20270..21163)
                     /gene="lipA"
                     /locus_tag="VM841_12050"
     CDS             complement(20270..21163)
                     /gene="lipA"
                     /locus_tag="VM841_12050"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVL90955.1"
                     /translation="MEQVAEGRKPSWLKIRATMGANFVDLKKIVGGERLHTVCEEARC
                     PNIYECWEDREATFLIGGNLCTRRCAFCDVATGKPAGYDTDEPRRVAESVERMGLRYA
                     VVTGVARDDLPDGGAWLFAETIRQVRARIEGCGVEVLVPDFKGDRDAIAAVCEADPEV
                     FAHNVETVPRLFGRIRPAFTYEGSLDVLRVARAILRPERVVKSNLIVGMGETHDEVIA
                     AMRDLREAGCDLLTIGQYLRPSDLQMPVARYVPPEDFAAWKAAGEAMGYAWVEAGPLV
                     RSSYHAGKQHRAAAARRPAAR"
     gene            complement(21222..21899)
                     /gene="lipB"
                     /locus_tag="VM841_12055"
     CDS             complement(21222..21899)
                     /gene="lipB"
                     /locus_tag="VM841_12055"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HVL90956.1"
                     /translation="MNPILASWVGRVRYEPAWALQRLVAELRAEGKLGDTLLLLEHEP
                     VYTLGRRGSEAEILMDPDERQRRGIETVRSDRGGLVTYHGPGQLIGYPIFNLGVAADV
                     RDFVCRIEKTMIGVAARFDVAAERIDGLPGVWVGDAKLGAIGVHISRGITTHGFALNV
                     ATDLDAFNGIVPCGIVDKTVTSLDVETGREVLMDDVVSATVEAAAEAFARPVSLTEMD
                     FEAIAGG"
     gene            21983..22735
                     /locus_tag="VM841_12060"
     CDS             21983..22735
                     /locus_tag="VM841_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90957.1"
                     /translation="MQINIEELSVGDTLRFGAAVRGSCDGASSLEDGATRVARLLHAA
                     LRDRHGEGTVVLSRVYKTHAAGDLPADLTAKATELLGKPPKRRTPCLVLLGTAGDRAE
                     WNDRRQSRGHQVIPLPSEDAIARLPMIATLIRQLGVPTGAILDSEPELLLDLRERVFN
                     VFHVEDAAASPSIPAKDFVADHGVRSAFGFGGLLLGSDLFAAVVFTRCPISRETAELF
                     KTLALNVKLALLPLASGPLFTETSDGEATVMS"
     gene            22735..24327
                     /locus_tag="VM841_12065"
     CDS             22735..24327
                     /locus_tag="VM841_12065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVL90958.1"
                     /translation="MGAREIEQLKSSVAALTHLLETHENVVAEFSDRLRVERDRLKAV
                     FRDIPAAVAVTRGGDNRFEFVNDFFVQTIGGRDVVGRTVREAFPELEGQGYFELLDGV
                     FSSGEPYRAAEARVMLDRGGGQPDEAYFTYTYVPLLDEDGKAEGILTHAVEVTELVRA
                     RERERREAEALQRSLLPGGLPFVAELDIAVRYQPGGGARVGGDWYDVFRLPDGRIALA
                     LGDVVGHGTRAASVMAQIRTAVRAYAFEGHEPRGVLNHTDRLLHSMGADDMATLVYGV
                     FDPAAETLTIASAGHPPPVFRPAGASDAEILSVVIAPPLGIQMGSYEQRTLQIGEDDV
                     VVLYTDGLVERRRESLDTGLERLRLRVTEAGSAEAATLCDAVLAGMLTDDVEDDVALL
                     VVRRLPAAGRELKLRLEAEPGHLAPLRQQLRGWLSTVEAPADIADDVMIAVGEAAANA
                     VEHAYRMSGGPIEVEAIVDADGLAIVIRDFGAWQQPRRRDRGLGLRMMRAFMDEVVVR
                     PTAEGTEILLRRKLRGNGGTAA"
     gene            24354..24683
                     /locus_tag="VM841_12070"
     CDS             24354..24683
                     /locus_tag="VM841_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL90959.1"
                     /translation="MNGEIAVATVVGEFDIGNCDEMSRQIQASCADALAIVLDLSETQ
                     YLDSAALRTLFTIARMFQGRGAPVVAVIPATGLVRKVTVVSGIDAVMPVAETRDEGLR
                     LAGGPVA"
     gene            complement(24680..26056)
                     /gene="lpdA"
                     /locus_tag="VM841_12075"
     CDS             complement(24680..26056)
                     /gene="lpdA"
                     /locus_tag="VM841_12075"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVL90960.1"
                     /translation="MPETYDVVVLGAGTGGYATALRAASLDLKVAMVERDKIGGTCLN
                     VGCIPTKALLHSADVYDTMRDAETFGVTASNVTYDWAGVQKRKDAVVERMIKGLASTL
                     KARKVETITGTGTITAKDTVTVDGTALGTKNIVIATGSKPKMIPGLEIGDRIITSDEA
                     LSMDHVPTSAVILGAGAIGVEFASLWASFGCKVTVVEMLPSLVPLEDPDAGKELARAF
                     RKRGITSMVGAKLEEAKAGADGVTCRVSVDGKTETIEADVLLVAVGRGPVTDGAGLES
                     IGVATDRGFVVVDEYCRTNVENVYAVGDVIPTLQLAHVSFAEGMLAAEQIAGKQVAPL
                     DYDAMPRATYCDPEVSSVGLTEPQAKEQGFEIETKSVGFGHIGKAAILNKTTGFCKIV
                     AEKGSGRILGVHMVGPHVTDLLAEAMLAYGWEATAGDIAPFIHPHPTLSEVFGETALA
                     LAGRPLHA"
     gene            26135..27184
                     /gene="gcvT"
                     /locus_tag="VM841_12080"
     CDS             26135..27184
                     /gene="gcvT"
                     /locus_tag="VM841_12080"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HVL90961.1"
                     /translation="MTKTSPLDAEHRALKAHMGAFGGWEMPISYAGTVAEHTAVRADA
                     GVFDVSHLGKILVTGDGAAALLDRALSNRYTDLPEGRARYTLMLDEDGGIVDDMIVYR
                     LDDDYLVVPNASNVDEVERRLRATDGAEAAIIERRDDAILALQGPRSRAALDAVIGDV
                     PMLGYMHMVRIGSFGIARSGYTGEHGYEIFVPPADAPGLWRAILDQNVTPCGLAARDT
                     LRTEMGYPLHGNDIDRDTLPAEAGLRWAIGKDKDAFAGKAAIESKEPGKTLVGITMTD
                     RVIPRRGCAVTANGQTVGTCTSGTFSPTLKIGIALASVAPGSVKDGDAVSVEVRGKTG
                     QGMVKKPPFVDATPR"
BASE COUNT         4308 a         9343 c         9390 g         4254 t
ORIGIN      
        1 ccgctgcgcg agccggacgg agaacgggtc gtcgtaggcg aagcggaaga tccgcatcgg
       61 cccatgcgag gagccgcggt cgtggtcaag gtcgaaccgg tccagcacca ccacatcgga
      121 gcggccggcg cgcgacagcg accacgcggc ggccgcgccc atcgcgccgg cgccgacgac
      181 ggcgaactcg cacgtttcca tagacccgga atgctatgcc caacgcttgc tcgtcttcgt
      241 aaggaccggc cggccgggcc gtagataccg gtagacgcgc gcttgccggg cgcgtcgtgg
      301 tgcacgccgc cgctgcgcga aggagggaac ccccgtcccc tccttcgtcg catttccggg
      361 ccgtatagac tccacttcac ttcgaaccga ccgtcggcag ggggccacga tggactttcg
      421 cgatacgccc gaggaagcgt cgttccgcaa agaggtgcgt gcctggttcc aggcaaacct
      481 tcccgaggga tggggctcga cgagcctgta catggagtcg cccgataagc gtatcgagtt
      541 cctccgcgac ttccagcgca agctctacga aggcgggtgg gccggcctgt cgtggccgaa
      601 ggagtacggc ggccgcggcg cgactctgat cgagcaggtg atcttcagcc aggaagcggc
      661 gcgcgcgaag gcgccacagc cgctgggcgt catcgggctc ggcatggccg ggccgacgat
      721 catcgcgcac ggaaccgacg agcagaagcg ccggtacctg gccaagatcc tgtcgggcga
      781 ggagatctgg tgccaaggct tctccgagcc gggctcggga tcggaccttg cgtcgctgaa
      841 ggccaccgcg gtgctcgacg gcgacgcata cgtcgtcaac ggacagaagg tatggacctc
      901 gtacgcgcac atcgcggact ggtgcatctt gctcgcccgg accgacaaca ccggcccgaa
      961 gcattcgggc atcacctatt tcttgatgga catgcacgcg ccgggcgtcg aggtgcggcc
     1021 gctgcgccag atcaccggcg acgcccagtt caacgagctg ttcctgaccg acgtgcgcat
     1081 cccgaaggag aacatcctcg gcgagatcaa cgacggctgg cgcatcgcga tgacgacgct
     1141 gctgcacgag cgcggcacgc tcggcttcgc gctggcggtc gcgggcggcg tcgcgctcga
     1201 cgagctggtc gcgttcgcca agaccgcccc gcgcgcgggc ggcggcgtcg cctacgacga
     1261 tcccgtcgtg cgccagcgca tcgcgaaact gcacgaggag gttcaggcga acctgctcaa
     1321 caactatcgc gctctcgcca cgatgatgaa gaccggcatc cccggtccgg aagggtcgct
     1381 gtccaagctg atgttcagcg agaccaacca ggagctcggc aagctcgccg tcgagatcgc
     1441 cgggggctac gggctgctgg aggcggggga gcggccggac gtggcgcgat ggcagtacgc
     1501 gcatctgcgc gcgcgcggca actcgatcga ggcggggacg agcgagatcc tccgcaacat
     1561 catcgccgag cgcgtgctcg gcctgcccaa gcacaagtaa ccgggggatc gggtcatgga
     1621 attcggactg agcgaagacc agcagacatt gaagaagtcc gcccgcgagt tcctcgaggg
     1681 cgaagccggc atcgcgctcg cgcggcgcat gctcgagcac gagtcgggat tcgagccgtc
     1741 gctgtggacg cacatggccg agctcggctg gatgggcatc gcgatccccg aatccgaagg
     1801 cgggctcggg ctgggaatgc tcgagctgtc gctgctgctc gaggagatgg gccgggtcgc
     1861 gctcccgtcg ccctacttct cgaccgtcgc gctcgccgtg ccggtggtcg catcgctggc
     1921 ggatgcagcc acgcgcagcg aggtgctcgg cgccgttgcg gccggggagc gcaagctcac
     1981 gctcgcctat gccgaagcgt ccggcgcgtt cgatccggcc gcgattgagg ccgccgcgca
     2041 gggcagcggg gatttgtgga cggtcaccgg tacgaagatg ttcgttcccg acgcgcacct
     2101 ggccgaccag atcgtcgtgc tcgcccgcac cggggtgggc gtcggcgcgt tcctggtgca
     2161 ggcggccgat tgcgagatct cgcagaacga gtcgctcgac cggacccgcc ggctcggcac
     2221 tgtgacgctc ggcggcgtgg ccgcgcggcc gctcggcggc gaggacgcct gggccgagat
     2281 cgagcgggcc cttgatcgcg ccgcggtcgg gctcgccgcc gaagccaccg gcgtcgccga
     2341 cgcggtaatg tccatgagcg tcgcctacgc caaggagcgg cagcagttcg gccgcgcgat
     2401 cggctcgttc caggccgtga gccacacttg cgccgacatg ctcgtcgccg ccgagagcgc
     2461 gcgcagcacc acgtactacg ccgcgtgggc gatcgacgaa ggcgcgcccg acgcgccgcg
     2521 tgcggccgcg accgcgaagg ccgcggccac gaccggcgcg atgcgcgtgt gcgaggacgg
     2581 catccaggtg cacggcggca tcggcttcac ctgggagcac gacatgcacc tgtggtatcg
     2641 ccgcgcgaag ctgtgcgagc tgctgctggg ccccgcgacc gtgtggcgcg aacgcatcgc
     2701 gtcgctgctg taggcgtggg caagcccaag ggcgagccgc gcgccgcgtg ggtcgaggac
     2761 gagacgccgc cggagaagaa gaagcgcgcc gcggcgatcc gaacgcgtct ggcgaaggag
     2821 tacccggacg cgcgctgcgc gctcaacttc agcaacccgc tcgagattct cgtcgccacc
     2881 gtgctgtccg cccagtgcac cgacgcgaag gtcaaccagg tcaccgagac gctgttcgag
     2941 aagtaccgca cggccgagga ttacctggcc gccaagcccg gcgagctcga gaaggacatt
     3001 cacgcgaccg gcttcttcaa tcagaaggcc aagtcgatcc gggggctgtc gcgtttgatc
     3061 gtcgaggagc acggcggtga ggtgccccgg acgatggagg agatcctgcg gctgccgggc
     3121 gtggcgcgaa agacgggcaa cgtcgtgctc ggaaacgcgt acggcgtcgt ggaagggatc
     3181 gccgtcgaca cgcatgttca tcgcttggcc tggcggctgg gcctgagcga ccaggacgac
     3241 ccggtcaagg tcgaacgcga cctgacggcg ctgtttccga agcggcgctg gctgaccttg
     3301 acctacctgc tgatcgagca cggccgcaac gtctgcttcg cccgcaaacc gcgatgcgac
     3361 gagtgcgtcg tcgcatcgcg ctgccccgca cgccggatgc cctgaccggt cgtcccttcc
     3421 cgagcaagga tcgcgggtag acttcccgat catgccacgc atctacacca agaccggaga
     3481 cgacgggacc acgggcctgc tctacggcgg gcgggtctcc aaggacgaca tccgaacgga
     3541 ggcctacggc acctccgacg aggccgtcgc cgcgctcggc gtggcccggg cccagacggc
     3601 ggacgtgacc ctggccgaga ccctggtgcg actgcaacgc gaactgttcg tggtcggggc
     3661 cgagctcgca accggcgaag gcgcctggga caagctcaag ccgggtgagt cgatcgtgga
     3721 cgagcctatg gtcgaggcgc tcgaggagct gatcgacgag ttcgtggcca agacgagaat
     3781 gcccgaggag ttcgtcgtgc ccggcaacac gtcggtcagc gcggcgatcg accttgcccg
     3841 ggcgatcgtc cgtcgatgcg agcgtgaggt caccacgatg acgaacgaag ggcttctgcc
     3901 cgatcgcgag tgccttcgct acctcaaccg gctgtcggac ctgctcttcg ttctggcacg
     3961 ttatgaagag gccggcttca ctccgctcca tccggcctga cccgtcacat cgaccgagca
     4021 aggaggaacc gtgaactcga gcaccatgcc gaaaaccgtt ctgatgctca ccgcggccgc
     4081 ggtgctgctg ggagcctgcg cgacgaagca gactcagccc gaaggcggcg gcagcggcgc
     4141 cgggcccggg gtcgccgtcc aggcgcccga gtccgtcaag ggcaacgtgg tcacgctcga
     4201 cctcaccgcc aagaacttcg aaatcgtcaa ggccgacggc gacacgtcgg gcaagaccgg
     4261 tcacttccac atcttcatcg accgcgagcc cgtggccgcc ggcgaagtga tcgcgcgtga
     4321 agccggcatc gtgcactcga cggacgcgaa gaccaagatc atgggcctgg cgcccggcga
     4381 gcacacgttc atcgtggtgc tcggcgacgg caaccacgcc cgggtcggcg acgcgcaggc
     4441 gaaggcgacc gtcaaggtcg aggggccgag cgttgacgcg agcgcgcccg cgacggtcaa
     4501 ggccggcgag gccgtcaccg tctcaatgaa gatcgacggc gcgaccctgg tcggcgccga
     4561 cggcgacacc tccggtaaga ccgggcacct gcacatcttc atcgaccgcg acccgatcct
     4621 cgaaggaccc atccctgtgg aagacgggat cgtgcatacg aaggagacca gtcacacgat
     4681 cgaggggctt gccgcgggag accacaccat ctgggtcgtg ctcggcgacg gaacgcacac
     4741 ctcgttcgcc gatccggtga tggacaaggt caccgtcacg gtcgcgtagc cggccgggaa
     4801 cgcgaaagcg ccgcccgcga agaggcggcg ctttcgcttc cggcgctacg tgtacgaggt
     4861 ctcgtcgtcg gggtagcggc cgtccttgac gtcgctcatc catcgcgtca ccgcgtcgcc
     4921 gatcacgccc cgcacgtccg cgtacttgcg gacgaacttc ggggcccagt cgttgatgcc
     4981 ggcgaggtcg ttgatcacga gcacctgcgc gtcggtgtcg gggccggcgc cgatcccgac
     5041 cgtaggtatc gacagcgctt tggtcacgct cgccccgagc gccgcgggca cgacctcgag
     5101 cacgatcgcg aagcagcccg cgtcctcgag ctccttggcc caccgcagga tgctcgcggc
     5161 ctgctcctcg gtccgcccct gcaccttgta gccgccgaac aggtgcaccg actgcggggt
     5221 gaggccgaga tggcccatga ccgggatccc gcgctcggcc agcttggccg ccagctcgat
     5281 gcgcggccct tcgatcttga ccgcgtgcgc gccggcttcc ttgaggaacc ggcccgcgtt
     5341 gcgcagcccg tcctcgtccg aggcctgata cgacatgaag ggcatgtcgc cgacgatgag
     5401 cgcgttccgt gatccgcgcg cgaccgcctt gcagtggtgg atcatctcgt ccatcgtcac
     5461 cggcaaggtg gtgtcgtagc cgaggatcgt gttggcgagc gaatcgccga ccagcagcac
     5521 gggcacgccc gcctggtcga ggatctgcgc ggtgggatag tcgtaggcgg tcagcatcgc
     5581 gaacttctcg ccgcgcgctt tgaacgccgc cagatcgtgg gtcgttacgg acatgcgtgc
     5641 cacctcctca cctacaggcc ctcgcgcgtg acgcccgtcc gcggtcaccg ggtcccatgg
     5701 cttgcctcat tgtgacagtc ccgccgcgtt cgcgcacgcg gcggttctcc gcctttgacg
     5761 tgcacgtctc aggcagcgcc gagaggggac ggcatgagca gctcgagctc gtatctcgga
     5821 gcgaggcacg atgacgcgcc ggctcgcatc atcagccggc cttccggctc gccgggccag
     5881 gcggtgcagg caagcgggcg cccggcgtgc ccggtccgcg gcgtgatccc ctcggatgcg
     5941 acccctgcgg acggatccat ggccgaactc gttgccgggg cccgagtcgc cggcgattgc
     6001 gtcattgcgg ggcgggttga cgacacgagc agggccagcg gaacggcgac gtatgcgagc
     6061 gctgcaacgg tcagcgcagc ggtcaaggcg cggccgcgga gccggcctcg tcgcggcacg
     6121 gatgccgacg gaggcggaag gagcggcccc tccaccgccg gagcgggcat cacccgtccc
     6181 ggccgggacc ccgtggccgg cgtcgcggga ggccggatcg cgagcgcggc ttgcgggctc
     6241 atcgcgtccc ccactttccg tcccgcagag tcgcgagcgc gtacggtgtc tgccagatca
     6301 tcacggtgct gtagaacagg gcgtacagaa ttccgaagag ccagtcgctc gagcgacgtc
     6361 gcatccggtg atagagcccg aacagcacgg ccatcagaac gatgccggtc gcgtagatcc
     6421 acgggtggcc ggcgttcatg gccgggcgca ccacgacgta gcgcaggaag acgacgggcg
     6481 ccatcaaggt cagcagcacg ccggtgtaga aagcccatgc ggcaagcacg ttccgcttcc
     6541 acatgaatcg gccggcgcgg aggctttcgc ggatccagga ttttttccac cggagctgct
     6601 gccgcatgaa ttggctcaac gtctccggca cgagcgtgga tgcggtcgcg tcgggcgcgt
     6661 agagcgtttt ccacttgttg atcaaaacgt ggttggtcag gctgcggtcg tcgccgtagg
     6721 tgctcgccgt atcggcgaag gtttggttga gccactcgtg cagcacgggt tcgaccgccg
     6781 cgcgccggta tccggagaag catcccgagg cgcacgtgac cgttccgaac agcgcctcgg
     6841 aggccttcac gaccttgaac gcgacgtagt agcgcacgga ctgcatccgg gtaagcacgt
     6901 tctggctcgc gttcgctacg tcggcatgac ccgcgacgca tccgacccgc tcgtcggcga
     6961 aataatccat catgcgccgg accgattccg gatcgaggaa gctgtcggaa tcgacgaaca
     7021 cgaccacgtc gccccgggag cggacgaacc cttcggccat cgcgaaccgc ttgccgcggt
     7081 tctccatcga gacgatccgc aaccggtccg gatggtcgcg ctgcacggcg cgcatcgcgg
     7141 gaagcgtccc gtcggtcgag ccgtcgtcca ccgcgatgat ctccaggcga tccgccggat
     7201 acgcggaagc cagagcccgt cgaagcgtct gctcgatcgc ggcttcttcg ttcttcgcgg
     7261 gcacgacgat cgagacggca ggcaaggcgt cgtcgacaac gacagctctc gtcggccggt
     7321 accgcgccgc aagcgcgaac ctcgagagca gatagacgcc gacgaggatc gcgtacgagc
     7381 cccagaagat ctcgcgctcg atgccgcgca gagtgaaaac cttcagggcg atcacgccgg
     7441 caacggtcgt cgcgaagatc gcgccgacga ggcggcgctg aaggcgtgga accgcgagcg
     7501 catccacggt gcgtttgcgc ggaaggcggc gcggccggac cagcgcagca ggctcaccca
     7561 gcatctcgcc ggggcgaagg acaccgtcgc tcacggcagc acccgattcg gccagccggg
     7621 caaccggtag gtcgcgtcga gcagaacggg ggcgctacgc agcagcgccg cgtcgaatcc
     7681 cggatggatc gtatgggcga ggatcagatc gtaggtttcg ggctgcagca ccgcgaccga
     7741 acgcatcttc acgccgagcg cgtcggtgaa gaccggcacc atcgggtcgt gatactccac
     7801 gatggctccc cgtgatcgca gcgacgcgat gatctcgccg gccggggact ctcgcacatc
     7861 ttcgacgccc ggcttgtagg cgacaccggc gacgagcacg cgtgcgtcgc gaaggcccgt
     7921 cccgcgatcc gagagcacgt cccggatgcg ggtcacgatg gcggcgggcc gttcggcgat
     7981 cgaagccatg gtccggtcca gcagcggcat cggcgttctg cgcgcctgca tctgccactg
     8041 caggtaccga ggatcgcacg ggatgcagtg cccgccgacc ccagggccgg gcgtgaagcg
     8101 catgaatccg tagggcttcg tggctgccgc gtcgatgacc tcgtcgatgc gcacgcccag
     8161 cgccgacgcg gccccggcca tctcgttggc gaatgcgatg ttgatcgccc ggaacgtgtt
     8221 ctcgtagagc ttggtcatct cggcggcctc gggcgaggag acaatgtgga tgtggggtgc
     8281 gatcgcgccg atcatccggg cggcgcgctc ggcgcattcc gccgtcgcgc ctcccaccac
     8341 gcgcggaacc gcgtcctgcg gaaagcgtcc gttgccggga tcgacccgct cgggtgagaa
     8401 cgcgagaaag acgtcccggc ctgcgtccag cccttcgttc gccagcggct cgatgagcag
     8461 ctcgcgcgtg gtcccgacat acgaggtcga ggtcaggacg atcgtctgtc ccggcgaggc
     8521 gttgcgcacg accgtcgcgc acgcgcgctt gagcggctcc aggtccggaa ccatgtgttc
     8581 gtccacggga gtgggcacgc acacgatcac ggtgccggcg cagcgaagga gcgtggggtc
     8641 ggcggtcaga cgaaacgacg gatcggaaag cgcccgggcg agccgggacc ggtcgccgtc
     8701 gagcaggtcc acctcgccgg tccggatgcg gcggaggcga ggttcgctgg tgtcgatgcc
     8761 gatgacgcca cggccgcggc ctgcataggc gagcgcggtc ggcaaaccca cgtatccgag
     8821 gcccacgatc gccacgatgt tggcgtcgac gtgggcggcg gccgccggat gcgtggcggc
     8881 gttgaacagc attcgagact cgactcgcac gaagatcccc cctggttcag gtgtgtcagg
     8941 aacaccatcg gtcggagttc aggctgagtt aagagcctcg ccaaaaattc gaaaaggcgc
     9001 ccagggtgga aagccccagc agcgcgcccg aggtccgttt tatccggcga gcgcgcgcgc
     9061 gatgtcgcgc atcgagacca tgccgaccgg ctggtcgccg tcgcagatgg gaaggtgccg
     9121 gaagccgccg tcgaccatca gttgcagcgc ttccttgacc gaggtcccgg gcgtggcggt
     9181 gccgggcttc ctcgtcatcc attccgcgac cggacgcacc ggggcgtcgt gggcgttgga
     9241 catcgcgcgt acgacgtcgc gttcggtcac gatgccgacg agccgcccct cgtcgatcac
     9301 catcgcgctg ccgacgcccc gctcgcccat gagtgtgctc gcctcggcga gggtggcctc
     9361 cggatcgacc gtgcacaggt cggtgctcat cagctcgtcc accttcatcg agggtccttt
     9421 ccccgtcccg gcttgcgccc gggaatcttc atcgcccatt cgcgatagcc gttggtgatc
     9481 ggcaggcgcc ggtcccgtcc gaaggcgcgc gccgtgatct tgatgccggg aggagcttgg
     9541 cgccgcttgt actcggccgt atcgaccatg cgcgcaacgc gcccgacggt tggctcgtcg
     9601 aatccggctt tcgcgatctc gtccaagccc gcgtctcgct ccacgtaggc ttcgaggatc
     9661 gggtcgagct cgtcgtaggg aggcagcgaa tcggtgtcga gctgaccttc gtgcagctcg
     9721 gccgtcggcg gcttgacgat cacgctctcc gggatcgccg ccgggccgcg ctcgttgcgc
     9781 cagcggcaga gcgtgtaaac gagcgtcttg ggcacgtcct tgatcaccgc gaacccgccg
     9841 gccatgtcgc cgtacagggt cgcgtagccc gtcgccatct cgctcttgtt cccggtcgtg
     9901 aggacgatcg cgccggtggc attggagatt cccatcagca gcgtgccgcg aatacgcgcc
     9961 tgcacgttgt cgagcgccgc gcccgggagt ggctcgccga gcgcatcgag cagtgacgcc
    10021 tcgtagccgg tcaccagagc gtcgatggga acgacgcgga agccgattcc cagattcgac
    10081 gcgagctcct tggcatcttc gatcgaggcg gtggaggtga cgcgggacgg catcgacacg
    10141 ccgagcacgt tgcccgcgcc gatcgcgtcc gccgcgatcg ccgccaccag cgacgaatcg
    10201 atcccgcccg acaagccgat caccgcttgc gcgaacccgt tcttgcgcag gtagtcgcgt
    10261 accccgagcg tcagcgccgc gtagacctcc tcctcgggtc ccatcggttc ggggatcgaa
    10321 cggggtggca cggcagggcg cttgcggctc cgggcgagcg gcttggagac gtcggtgacc
    10381 ggcgccttcg acgccgtctt tcgcggcgcg gccggcaggg acagatcgca caccaccagg
    10441 tcctcggcga actgggcacc gcgcgcgagc agatgaccgt tcgggtcgaa cacgagtgtc
    10501 tcgccgtcga agatcagctc gtcctgaccg ccgacggttt gcagataggc gaagaagatc
    10561 ccgttgtcgc gggcccgctg gcggaacagg atggtgcgtt cgaggccctt gccgcggtgg
    10621 tacggagagg cgttgacgtt caccaccacc tcggcgccgg cacgggcgag cgagcgcacc
    10681 ggaccgtcgc cggcccaggc gtcctcgcaa atgctgatcc cgatctgcac gccgcgaacg
    10741 cgaaacagcg ggaacgccga gccggcaacg aagtagcgct tctcgtcgaa gacgccgtaa
    10801 ttcggcaggc ggaccttccg gtagacgccg cgaactttgc cgcgtgcgag cagcgctgcg
    10861 gcgttgtaga gaccgccgtc gtcgcggtcc acgaagccga cgatcgccgc cgcgtcgccc
    10921 acgtccttgg cgaggcgggc taggacggcg aggttctcgt ccacgaacga gcgcttcaaa
    10981 aggaggtctt ccggcggata gccggtcagc gtcagctcgg gaaaggcgac caagtccgcg
    11041 ccggcggcct cggccttggc aagccaggaa cggaccgcga gcgcgttgcc tcgcagatcc
    11101 ccgaccgtcg ggttgatctg cgcgagcgcg agccgcagtg ttcgcatgac gtcccggaaa
    11161 gcctagcatc cgctcctcgg cgttcggtag ggcgggtaga ctgctcgtca tggacgccgc
    11221 gcgcgagcag atcgactacg ttcttcgcac cgtcgaggaa cgcgacattc gcttcatccg
    11281 gctgtggttc accgacgtgc tgggctttct gaaggcgttc gcgatcacac ccgccgagct
    11341 ggaggacgcg ttcgaggagg gcatgtcctt cgacggcagc gcgatcgagg gctacgcgcg
    11401 cgtccaggag tcggacatgc tggcgcgccc ggatccgtcc acgttccagg cgctgccgca
    11461 ggccaagggc gacctcgagg cgcggatgtt ctgcgacatc accacgcccg acggcgcgcc
    11521 gttcccgggc gatccgcgcg ccgtgctgcg ccgcaacctc gagcgcgcga gcgcgaaggg
    11581 attcacgttc tacgtgcatc ccgagatgga gttcttcctg ttcaaggatt ccaagacacc
    11641 cgtgccgctc gaccagggct cctacttcga tctcacgccg ctggactccg cgcaggattt
    11701 tcggcgccgc gcgatccagg cgctcgaggc gatgggcatc agcgtcgagt actcgcacca
    11761 cgaggtcgcg ccgtcgcagc acgagatcga tctacgattc gccgacgcgc tcacgatggc
    11821 cgacaacatc atgacctacc ggctcgtggt caaagaggtc gcccagcaga tgggcctgca
    11881 cgcgaccttc atgcccaagc cgctgcaagg cgagtggggg agcggcatgc acacccacgt
    11941 gtcgctgttc gaaggcgatc gaaacgcgtt cttcgatgcg cgcaccgccg gtcatctgtc
    12001 gaaagtcggc cgcgcgttcg tcgccggggt tctgcgtcac gcccgcgaga tcacggccgt
    12061 gacgaaccag tgggtcaact cctacaagcg gctcatcccc ggctacgagg ctccggccta
    12121 cgtgtgctgg agccgccatc tgcgcggcgc gctcgtgcgg gtgccgggcg cgaagccgac
    12181 caaggcttcg agcgcccgca tcgagattcg ctcgcccgat ccggcgtgca acccgtacct
    12241 gaccctcagc gtgatcctgg ccgccgggct gcgcggcctc gacgaaggct acgagcttgc
    12301 cgatgaggtc gtggacgacg tggacacgat gagcgaggag gagcgagcca agcgcggcat
    12361 cgtgccgctg ccgcaagatt tgaacgaggc cgtcgacgag ctcgagcgca gcgagctcgt
    12421 cgcggacgcc ctcggcgagc acgtcttcga gcagttcatc cgaaacaagc gcgccgagtg
    12481 ggacacgttc aaagcctacg tcagccccta cgaacgcgaa cgctacctgc cgttgctgta
    12541 gccgctcggc gcgtcactcg ggccgcgggg taggctcatg gtcgaaccga tggccgagcc
    12601 cacgtccctc tccgagatgc acgccgcgct cgcgcgtgcc ggtttcgctc gtccggtaga
    12661 cgcggcccgc catctccagt cggtggcgct cggcccgaag ggcatggacc gggtcgtcgc
    12721 ggcggccgcc gacgcgcccg accccgactt ggcgatcgcg cagatctgtc gcttcatcgc
    12781 gatgaccggc cgcgtgcctt cggccgccgt gctccagcgg ctggccgccg ttttgggtct
    12841 gtccacgagc ctcggcgcct tcctcgcccg gcatcccgac gccgcggaca tgctccgcga
    12901 ggatgcgctc gccgcgccgc gcaccgtcgc cacgctccgg gcgcgggccc gcaagctcgt
    12961 ggccaccgac gacgatccgc tcacggcgct tcggttgttc aagcgccgcg agttgctgcg
    13021 catcgcggcc cgcgacctcg ccggcggcgc cgcggtcgag gagatcgcgg cggagctggc
    13081 cgcgctcgcc gaagcgacgc tgcaaggcgc gctcgacgtg ctcgtagcgc agaacacgcc
    13141 gccgccgggc acgcgcttca cggtgatcgg catgggcaag ctcggcggaa gcgagctcaa
    13201 ctacgcgagc gacatcgacg tcgtgttcgt cttcgactcg cccgacgagg ccgaggccgg
    13261 cccctgggcc aaccgcatcg ccgaagggct gatcaagaac ctcgccgcga cgaccgagga
    13321 ggggcaggcg ttccgtgtcg acgcgaacct ccggccggaa gggcgcgacg gcgcgctggc
    13381 gcgatcgttg accgcgttca aggcgtactg ggagcgctgg gccaagccgt gggagttcca
    13441 ggccctcatc aaagcgcgtc ccgtcgccgg cgacgaggtg ctcggcgccg agttctgtgc
    13501 cgccgccgcc gacgtcgtct atccggagcg gctcgcgccc gaagccgtgc gcgagatccg
    13561 cgcgatgaag gcccgcgccg agcgcgtgat cgcgcagcgg gggctgggca accgcgagat
    13621 caagcgcgcg cccggcggca tccgcgacat cgagttcgcc gtgcagctgc tgcagctcgt
    13681 gcacggccgc cgtgacgcgt cgctgcgcgg gggcggtacg ctgcccggcc tggccgcgct
    13741 ctcacgcggc gcgtacgtcg gcgacgacga cgcgggcgag ctcgcgtccg cgtacgtgtt
    13801 ccttcgccgc atcgagcacc ggctccagct cgcgcaggag cgtcagacgc ataccgttcc
    13861 cgaggacgag acgcgtcggc gaacgcttgc gcgctcgatg ggctttcggg acggcggcac
    13921 cgagacggcg ctggaggcgt tcgatgcggc gtggcgggac atccaggccg tggtgcgccg
    13981 catccacgag aagctgttct accggccgct gctggagcgg ttcgcgcagg cgccggcgct
    14041 cgcgccggac gcggcgcagg agcggctcga ggcgctcggc ttccgctcgc ccggccgggc
    14101 tctgaagttc ctcggcgacc tgacggccgg cttgtcgcgg cgctcgcagc tgatgcgcac
    14161 gcttctgccg gtgatgctcg actggatgag cgactcgccg gatccggacc tggcggtgtc
    14221 gaacttccgg gacgtcgcgc tcgcgctcgg cggcaacccg gcggcgctcg ccgcgctgcg
    14281 cgactcgccg cccgtcgtcg agctgctctg ccgcgtcctc ggcacctccc gtctgctggc
    14341 cgagcacctg ctgcacacgc ccgagctggt gcccgcgctc gccgagcaaa aggcgctcgc
    14401 gcgcaagggc cgcgacaagc tgatgcgcga ggcggccgag cacatcgaat ggcgcggcga
    14461 tcccgacgcc cgcgatgcgg ccgtgcgccg cttcaagcgc cgcgagatgc ttcgcatcgc
    14521 ctgccgcgac ctcgccggcg gcgtcagccc cgacgacatc ggccgcgagc tgtcgcacct
    14581 ggccgaggcg gcgctcgaag ccgcgctcgg gtcgctccac gaagagcatc cgccccccgc
    14641 cggggcgcgc ttcgcggtga tcggcatggg caagctgggc ggtgaggaac tcaactacgc
    14701 gagcgacatc gacgtcatgt tcgtctacga cgcgcccgac gagaagggcg cgcacaagtg
    14761 ggcgacgatg ctcgccgaag ggctgctcca gcggctcgcg gccacgaccg aggaggggca
    14821 ggcgttcccg gtggatgcgc agcttcgccc cgagggcaag gacggtccgc tcgcgcgctc
    14881 gctcaccgcc taccgcgcct actacgagcg ttgggccaag acctgggagt tccaggcgct
    14941 cgtcaaagcc cggccggttg ccggcgaccc gatgctcggc cagcagttcg tcgatctgat
    15001 ccgtccgttc gtgtatcccg agcggctcgc gcccgagaag gtgcgcgaca tccggtcgat
    15061 gaaggcccgc atcgagcgcg aacggctcgg cccgcgccaa gaccccgcga cgcagatgaa
    15121 ggtcgggcgg ggcggcacca tcgacatcga gttcaccgtg cagctgctcc agctcatgca
    15181 cggcgcgcat catccgagca tccgggtgca aggcaccgtg ccggcgatct ccgcggccgc
    15241 cgagcgccaa ctgctgacca tcgagcaggc gaagtggctt ctggaggcat atcggttcgt
    15301 caaccgcacc cgcaacaccc tctacctgat ccgcggacgc gcgatggacg cgctgccggc
    15361 caagccggag gaggccgagg tgctcgctcg cgcgctcggc taccccgggc cgggcgcccg
    15421 cgtgcagttc ggcgaggact accgccgggt cacgcggcgc gcgcgacagg tttgcgagga
    15481 cgtcttctac ggaaaaaggc caacccgatg actcgcagaa aaacgacctg aaggagagac
    15541 catgagcgat cgccacgtgg acgtcgaagg ccgtcggatc tacattcgcg agcacggcaa
    15601 cggcgcgccg ctggttctcc ttcacggttt tccccagacg ggggaggcgt ggaagcaggt
    15661 cgcggacaag ctgtcgggcc gcaaccgggt cttcgtgccc gatcttcccg gattcgggcg
    15721 cagcgacgca gccgtcgcct ccgacatgca gaccgtgtcc cgcacgctcg ttcgcgcgct
    15781 cgccgcggcg ggcgcggaga agttcgcgct cgtcgggcac gactggggcg gttcgatcgc
    15841 gctgcgcatc gcgctcgacc atccccaggc cgtggagaag ctcgtcgtgg tcaatgcgcc
    15901 gttccgctcg cttgatctca cgcgtggctg gcacttcctg gcgttcaacg ttccgctgat
    15961 cccagaggcg gccatggtgc tgttcggcaa gcgccttgtg ccgatgctga tcaaggccgg
    16021 atccaagaac aagcaggcct tcgacgccga gaccatgaaa ggctacggcg aggcgttctc
    16081 ggatctgggt cacgcccgca gcacgctcgc ctactaccgc accatcacgc gctcgacgat
    16141 catgcgcggc gtgcgcggcg cgttggacaa gctgccggtg ccgatgcccg gcggacgcgg
    16201 gacccacgat aacgaagccg ggccgcgtcg cgttcaggtc ccgaccttga tcgtgtgggg
    16261 cgagcgcgat ccggtgctgc cgccctcgct catcccgacg atcgaacgtg atgtggatga
    16321 cgtcacggtg gtgcggctgc ccgacatcgg gcatttcgtt cccgaggagg cgccgtccga
    16381 gcttgcgcac gagatcgacc gcttcctgaa cccgcatcga ggcgagtagg ccgcgcccgg
    16441 ctccgcgcgt tccggcccgt tcgtcgcact ggactggggc cggcggccga tcatgctgtc
    16501 gggcatggac ctcgtacgtt ccgcgcgcgg ccgtcccgac gagacacggc gccgcatgct
    16561 cgacccgatg ttcgccgacg atccgggcca gcgtgcctgg ctcgagggca cgttgctgcg
    16621 gcccggccag gtggcgctgc tgctccaggt gagccgccgc agcgtcgcgg cgtgggcgcg
    16681 ggagggccgg ctgccctcca ttaccacgcc cggcgggcac cgccgcttcc gggccgacga
    16741 cgtgcggcgc ctcgtcgatt cgctgacggt caccgttccg gccggatcga caccggaccg
    16801 actcgccaag aatcagacgg tttccgagtg acccagggcc gcttcagaag gtttgtcctg
    16861 attttggctc gtaagatctc cctatgagca ccgtattccc tatggaaacg ctagaaaccc
    16921 tgggagcgtc ccagggatcg agggggggga tcatgatgaa gaagatcttt gcggcggccg
    16981 tcgtggccgc gggtctgttc gcttcgctgc cggcgtcggc cgacgtcatc gacatcgcgg
    17041 gaacgatcta tgtcgacgac tcggacgcca ccgtctggca ggagagcaac ggcgtcgagg
    17101 gcctgcagcg cgaggccgac ggcgagaccc ctgccgactc gcagcttctg tagtccgcgc
    17161 atccccgcgc acgtgaaggg cccggcctgc cggccgggcc cttgcgcttt cgtcgcggcg
    17221 ccgtcaggcg tgccgacggc gttcgacggt tgcctcaggg cgacctcgcc gcacctcgtc
    17281 gaggcggacc ggctcggcgg cactcagcac tctgaccacc tgcttcctct cgtcgggccc
    17341 cgcaacggcg acgccgagcg acacgcccgc ctcggccgcc atcggttcgg cggggctttt
    17401 cgcggccgcg ccgcctccgg cgacgaagcc gaaggctccg ccggcaaccg ctccgacgat
    17461 ggtcacgatc atcagcttca ccgcgatgac caggtcgccc atggggatca gcgagaacgg
    17521 ggcggcgagg atcgcgccga ccaccgccca gacggccgtg ccccatgccg caccctttgc
    17581 catctccggc gtatacagcc cgacggatgg gccggcgaag gaacgtgcgt cttcttcggc
    17641 catctcggcg cgcagggcgg cgacgtgact tgcgtcgtcg ccgacgcgaa ccgtgacgcc
    17701 gggcaccgcg gccgttaccg ctgcggcggc gcgctcggct tcggccgccg agcggaagac
    17761 ggcgacgacg ccgtcgatag gatcgtgctc cgttcgatgg gggactcggg ctttctgccg
    17821 agcccgttcg gccgcgcggt cccagtcggg ctcgcggccg ctgccgggtg tgcggtcagt
    17881 tgtcatggtc gccttcgttc ccccgatggc aggcgatgaa cccgaagcaa gcggcctcct
    17941 caccggtccg gaacgccgga aggggcccga aggccccttc cgtttgcgat gcagcgactt
    18001 agatgtcgaa atacagcgcg aactcgtgcg gatgcgggcg gagccgcatc gcgtcgacct
    18061 cgtgcaaccg cttgtagttg atccaggttt cgatgacgtc ctcggtgaac acgccgcctt
    18121 ccagcaggaa gtcgtggtcc gcctcgagcg cgtcgagcac ctcttcgagc gagcccggca
    18181 ccgactgcac ctgcgccttg tcctcgggcg ccagatcgta gagatctttg tccatcggct
    18241 ccggcggctc gatcttgttg cgcacgccgt cgaggccggc ctgcaggcac gccgcgaacg
    18301 ccaggtacgg gttgcacgac gggtcgggcg tgcggaattc gatgcggcgc gccgccggcg
    18361 cgttggagta cgtcgggatg cgcacgcacg cggaccggtt gcgcgaggag tacacgaggt
    18421 agatcggcgc ctcgtagccc ggcaccagac ggcggtacga gttcgtcgtc gggttcgtga
    18481 acgcgagcag cgccggcgcg tgctgcagca ggccgccgat gtagtagcgg gccatgtccg
    18541 acagcatcgc gtagccggtc tcgtcgtaga acaggttctc gccgttgcgc cacagcgact
    18601 gatgggtgtg catgcccgag ccgttgtcca tgaacagcgg cttcggcatg aacgtcgccg
    18661 tcttgccgtt gcgccaggcg acgttcttga ccgcgtactt gtagtccatc acgcgatcgg
    18721 cggtctgcag cagctcgccg tagcgcatgt cgatctcggc ctggccggcg gtgccgacct
    18781 cgtggtgctg cacctcgatg tggatgccca gcttttgcat ctccaggacc atctcggtgc
    18841 gcaggtcctg atactggtcc atcggcggga ccgggaagta gccttccttg tagcgcggac
    18901 ggtatccgcg cgcgcccggg ttgcccgagc cccacgcgcc ctcggtcgag tcgaggtggt
    18961 agtagccctc gtgctggttc tggtcgaacc gcgcctcgtc gaagatgtag aactcggcct
    19021 cgggacccca gtaggacacg tccgcgatgc ccgacgcctt caggtgatcc tcggccttct
    19081 ttgcgatatt gcgcgggtcg cgcgagtagg actcgccggt gatcgggtcc ttgatgaagc
    19141 agtacatgaa cagggtcgga tgggcaagga acgggtccat cttcgccgtg gtcggatccg
    19201 ggatcaggag catgtccgac tcttggatcg cctggaaacc gcggatcgac gacccgtcga
    19261 agccgaagcc gttctcgaag gacctctcgt ccaattccga gatcggcacg gagaagtgct
    19321 gggtgacgcc cggcaggtcg gtgaagcgaa gatccaccat caccgcctcg gaatccttgg
    19381 ccagcttcag tgcgtctttc gcggtctcag gcacgatgcc cctcccttga acggttcctt
    19441 cggcgcgagc ctaacgctcg tccccacgtc gcggcaaagc aggaaactcg caggtcacgg
    19501 tagcaggagc cgtatttccg ggccgtttcc cgtctgtaaa ttccttgtta cgggcgcggt
    19561 ccggtcctag gcgagcatct gatgcgctcg atgataacat cacgcatcat gcgcacgacg
    19621 atcgaccttg acgcgacggt gctggccgag ctcaagcggc gcgcgaagcg cgagtcgaag
    19681 tccctcgggc agctcgccag cgagctgctg gcggcggccc tgaagcagca gtccccgggc
    19741 aagcgatccg cgatccgctt tcacaccgcc gcgatggggg ccagagtcga tctggaagac
    19801 aaagacgcgc tctggcggac gctcgaggac cgatgagcag cgcgatcgac gcgaatctgc
    19861 tcttgtacgc atcggatcga tcgagcccct tccacgagcg cgcgctcgag accgtcacga
    19921 tgctcgcggc aggaccggcg ctgctgtacc tgttctggcc ggtggcgatg gcctaccttc
    19981 ggatcgcgac gcacccgggg gtgttcgccc ggccgctgcc gtggcgcgcc gcgtccgaga
    20041 acatccgcgg gctgctcgaa cgcgacaacg tccgctcgcc cggggaggat gaaggattct
    20101 ggccgttgtt cgccgatacg gccgacgccg ccggcgtacg cggcagtctc gtccccgacg
    20161 cccacctggt cgccctgatg cgccgttccg gtgtgacaac cctgtggacc catgaccgcg
    20221 acttccgccg cttcgacggt atcacgctgc aggatccgtt cgccggcgcc tagcgggcag
    20281 ccgggcggcg cgcggctgcg gcgcggtgtt gcttgccggc gtggtagctg gagcggacga
    20341 gaggaccggc ctccacccag gcgtagccca tggcctcacc ggcggccttc cacgccgcga
    20401 agtcctcggg gggcacgtag cgggcgaccg gcatctgcag atcgctgggc cgcaggtatt
    20461 ggccgatggt gagcagatcg cagccggctt cgcgcagatc gcgcatcgcg gcgatgacct
    20521 cgtcgtgggt ctcgcccatg ccgacaatca ggttcgattt cacgacgcgt tcgggtcgca
    20581 gtatcgcgcg ggcgacgcgc agcacgtcga gcgatccttc ataggtgaac gcgggccgga
    20641 tgcggccgaa taggcgcggc acggtctcga cgttgtgcgc gaacacttcc gggtcggctt
    20701 cgcacacggc ggcgatcgcg tcgcggtcgc ctttgaagtc cggcacgagc acctcgacgc
    20761 cgcacccttc gatccgcgcg cgcacctgcc ggatcgtctc ggcgaaaagc cacgcaccgc
    20821 cgtcgggcag gtcgtcgcgc gcgacgccgg tcacgaccgc gtaccgcaat cccatccgct
    20881 cgaccgattc ggcgacgcgg cgcggctcgt cggtgtcgta gccggccggc ttgccggttg
    20941 cgacgtcgca gaacgcgcag cggcgcgtgc acaggttgcc gccgatcaga aacgtcgcct
    21001 cgcgatcctc ccagcattcg tagatgttgg ggcaccgcgc ctcttcgcac acggtgtgga
    21061 gccgctcgcc gccgacgatc ttcttgaggt ccacaaagtt cgcgcccatc gtcgcgcgga
    21121 tcttcagcca cgacggcttg cggccttcgg caacctgctc catcggcagg ttccgcgccg
    21181 gcccttgcac ggtcggcagc agcggcagat gcttgcgcgt gtcagccacc ggcgatcgcc
    21241 tcgaagtcca tctcggtgag cgagaccggc cgcgcgaatg cctcggcggc ggcctcgacg
    21301 gttgcggaaa ccacatcgtc catcaagacc tcgcggcccg tctccacgtc cagcgacgtc
    21361 acggtcttgt ccacgatgcc gcacggcacg atgccgttga acgcatccag atcggtagcc
    21421 acgttcagcg cgaaaccgtg cgtggtgatg ccgcgcgaga tgtgcacgcc gatcgccccc
    21481 agcttcgcgt cgcccaccca cacgccgggc aggccatcga tccgttcggc ggcgacgtcg
    21541 aatcgcgccg ccacgccgat catcgtcttt tcgatgcggc acacgaagtc gcgcacgtcg
    21601 gcggcgacgc ccaggttgaa gatcggatag ccgatcagct ggcccgggcc gtgataggtc
    21661 accaaaccgc ctcggtcgct gcgaaccgtc tcgatgccgc gccgctgccg ctcgtcgggg
    21721 tccatcagga tctcggcctc gctgccgcgc cggccgagcg tatagacggg ctcgtgctcg
    21781 agcagcagca gcgtgtcgcc cagtttgcct tcggcccgca gctcggcgac cagccgctgg
    21841 agagcccagg cgggctcata ccggacgcga cccacccagg acgctaggat cggattcacg
    21901 ccgaccactt tacgcggcac gagccgtccg ggcggatcgg gtttcgagct cggcaattcg
    21961 ggatataggg gaccagaagg tcatgcagat caatatcgaa gagctctcgg tgggggatac
    22021 gcttcgtttc ggagccgcgg tgcgcgggtc ctgcgacggg gcatcctcgt tggaggacgg
    22081 cgcgacccgg gtggcgcgac tgctccatgc cgcgctgcgc gaccgccacg gcgaaggcac
    22141 cgtcgtgctg tcccgcgtct acaaaacaca cgccgccggt gacctccccg ccgatctgac
    22201 cgccaaggcg accgagctgt tgggcaagcc accgaagcgg cgcaccccct gcctggttct
    22261 gctggggacc gccggcgacc gggccgagtg gaacgatcgg aggcaatccc gaggccatca
    22321 ggtgattccg ctgccgtcgg aggatgccat cgctcgtctt ccgatgattg cgacgttgat
    22381 ccggcagctc ggcgtgccga ccggcgccat tctggatagc gagccggagc tgctgctgga
    22441 tctgcgcgag cgggtcttca acgtcttcca tgtcgaagac gcggccgcaa gtccgagcat
    22501 ccccgccaaa gatttcgtcg ccgatcacgg cgtgcgctcc gcattcgggt tcgggggcct
    22561 tctgctgggc agcgaccttt tcgccgccgt cgtcttcact cgatgcccga tctctcgcga
    22621 aacagccgag ctgttcaaga cgcttgcgct gaacgtgaag ctggcattgc tgccgctggc
    22681 ctcgggcccg ctattcaccg agacttccga cggggaagcg acggtgatgt cctgatggga
    22741 gcccgcgaga tcgaacagct caagtccagc gtcgcagcgc tgacccatct gcttgagacg
    22801 cacgagaacg tcgtcgccga attcagcgac cgtcttcgtg tggagcggga tcgcctgaag
    22861 gccgtgttcc gtgatatccc ggctgccgtg gcggtcacgc gcggcggcga caaccggttc
    22921 gagttcgtca acgacttctt cgtgcagacc atcggcggcc gcgacgtggt gggccggacc
    22981 gttcgcgagg cgtttccgga gctggagggg caaggctatt tcgagcttct cgatggggtg
    23041 ttctcgagcg gggagccgta ccgagccgcg gaagcccgcg tgatgctcga tcgcggcggt
    23101 ggccagccgg acgaggcgta cttcacgtat acctatgtgc cactgctgga cgaggacggt
    23161 aaggccgagg gcatcctgac ccacgccgtc gaagtgaccg agctggttcg cgctcgcgaa
    23221 cgcgaacggc gcgaagccga ggcgttgcag cgaagccttc tgccgggcgg ccttccgttc
    23281 gtggccgagc tggacatcgc ggtgcgatac cagcccggtg gaggcgcccg tgtcggcggc
    23341 gactggtacg acgtgtttcg gctgccggac gggcggatcg ccctggcgct gggggacgtg
    23401 gtcgggcacg ggactcgcgc ggcgtcggtc atggcgcaga tccgcaccgc cgttcgcgcc
    23461 tacgcattcg aaggacacga gccgcgcggc gtgctcaacc acaccgaccg gctgctgcat
    23521 tcgatgggcg ccgacgacat ggcaacgttg gtctacggcg tgttcgatcc cgcggccgag
    23581 acgctcacga tcgcctcggc agggcatccg ccgccggtgt ttcgtcccgc aggcgcatcc
    23641 gatgccgaga tcctgtcggt cgtcatcgcg ccgccgctcg gcatccagat gggcagctac
    23701 gagcagcgca cgttgcagat cggcgaagat gatgtcgtcg tgctctacac cgacgggctg
    23761 gtcgagcgcc gccgcgagtc gctcgacacc ggcctggaac gcctgcggct gcgcgtaacg
    23821 gaggcgggtt ccgccgaggc ggccacgctc tgcgacgcgg ttctggccgg gatgctcacc
    23881 gacgacgtcg aagacgatgt cgccttgctg gtggtccgtc gcctgcccgc cgcagggcgc
    23941 gagctgaagt tgcgtctgga ggccgagccg ggccatcttg cgccgctccg tcagcagctt
    24001 cgcggctggc tctcgaccgt cgaagcgccc gccgacatcg ccgacgacgt gatgatcgcc
    24061 gtcggagaag ccgccgcaaa cgcggtcgag cacgcgtatc gcatgagcgg cggaccgatc
    24121 gaggtggaag cgatcgtgga cgccgacggg ctcgcgatcg tcatccgaga cttcggagcc
    24181 tggcagcagc cccgccggcg tgaccggggt ttgggattgc gcatgatgcg tgcgttcatg
    24241 gacgaggtcg tcgtgcggcc gacggccgaa gggaccgaga ttcttctccg gcggaagctt
    24301 cgaggaaatg gggggactgc agcgtgacga aactgtccga agtccggtgt gcgatgaacg
    24361 gcgaaatcgc cgtcgcaacc gtggtcggcg aattcgacat cggcaattgc gacgagatga
    24421 gccgccagat ccaagcctcg tgcgcggacg cgctggccat cgtgctcgac ctgagcgaaa
    24481 cccagtatct ggacagcgcc gcgctgcgca cgctgttcac cattgcgcgc atgttccaag
    24541 gccgcggcgc gcccgtcgtg gcggtcatcc ccgccacggg cctggtccgc aaggtcaccg
    24601 tcgtatcggg aatcgatgcc gtgatgccgg tcgccgagac ccgcgacgaa ggcctgcgcc
    24661 tggccggcgg ccccgtcgcc taggcgtgca gcggccggcc ggccaaggcc agcgcggtct
    24721 ctccgaacac ttccgacagc gtcgggtgcg ggtgaatgaa gggcgcgatg tctcccgcgg
    24781 tggcttccca cccgtaggcc agcatcgctt cggcgagcag gtcggtgacg tgcgggccga
    24841 ccatgtgcac gcctaggatc cggccggatc ctttctcggc gacaatcttg cagaagccgg
    24901 tggtcttgtt caggatcgcg gctttgccga tgtggccgaa gccgaccgac ttggtctcga
    24961 tctcgaagcc ttgttccttc gcctgcggtt cggtcaagcc gaccgaggag acctcgggat
    25021 cgcagtaggt ggcgcgcggc atcgcgtcgt agtcgagcgg ggcaacctgc ttgccggcga
    25081 tctgttcggc ggcgagcatg ccttcggcga acgacacgtg cgcaagctgc agcgtcggga
    25141 tcacgtcgcc gaccgcgtag acgttctcga cgttggtgcg gcagtactcg tcgaccacga
    25201 cgaagccccg atccgtcgca acgccgatcg actccagacc cgcgccgtcg gtcacgggtc
    25261 cgcggcccac cgcgaccagc agcacgtcgg cttcgatcgt ctcggttttg ccgtccacgc
    25321 tgacacggca cgtgacgccg tccgcgccgg ctttggcctc ttccagcttc gcgccgacca
    25381 tcgacgtgat gccgcgcttg cggaacgcgc gcgccagctc cttgccggcg tcggggtcct
    25441 ccagcggcac cagcgacggc agcatctcga cgacggtgac cttgcagccg aacgaggccc
    25501 atagcgaggc gaactcgacg ccgatcgcac cggcgcccaa gatcaccgcg ctggtcggca
    25561 cgtggtccat cgacagcgcc tcgtcgctgg tgatgatccg gtcaccgatc tccaggccgg
    25621 ggatcatctt cggcttcgag ccggtcgcga tcacgatgtt cttggtcccc agcgccgtgc
    25681 cgtcaaccgt cacggtgtct ttcgcggtga tcgttcccgt gccggtgatc gtttcgacct
    25741 tgcgggcctt cagcgtcgaa gccaggcctt tgatcatgcg ctcgaccacg gcgtccttgc
    25801 gtttctgcac gcccgcccag tcgtaggtga cgttcgaggc ggtcacgccg aacgtttcgg
    25861 cgtcgcgcat ggtgtcgtag acgtcggccg agtggagcag cgccttggtg gggatgcagc
    25921 cgacgttcag gcaggtcccg ccgatcttgt cgcgctcgac catcgcgacc ttcagatcca
    25981 gcgacgcggc gcgcagcgcc gtcgcgtacc cgccggttcc ggcgcccagc accaccacgt
    26041 cgtaggtctc gggcatttgc tcgctcctca tcgatcggac ggaccatcct catgctaccc
    26101 atacccgccg cggccggtat aggctcgcgc cgtcatgacc aagacttcgc cgctggatgc
    26161 cgagcaccgc gcgctgaagg cccatatggg cgccttcggc ggctgggaga tgccgatctc
    26221 ctatgcgggg acggtcgccg agcacaccgc cgtgcgcgcc gacgccggcg tgttcgacgt
    26281 ctcccacttg ggcaagatcc tggtcaccgg cgacggcgct gcggcgctgc tggaccgggc
    26341 cttgtccaat cgctatacgg atctgcccga gggccgagcg cgctacacgc tgatgctcga
    26401 cgaggacggc gggatcgttg acgacatgat cgtctaccgg ctggatgacg actatttggt
    26461 ggtgcccaac gcatccaacg tggacgaggt cgaacgccgg ctgcgcgcca ccgacggcgc
    26521 cgaggccgcg atcatcgaac ggcgcgacga cgcgattctg gcgctgcaag gtccccggtc
    26581 gcgcgcggcg ctggacgcgg tcatcggcga cgtcccgatg ctcggctaca tgcacatggt
    26641 gcgcatcggc tcgttcggga tcgcgcgcag cggctacacc ggcgagcacg gctacgagat
    26701 cttcgtgcct ccggcggatg cgcctgggct ctggcgcgcg atccttgacc agaacgtcac
    26761 gccctgcggc ctggcggccc gagacacgct gcgcaccgag atgggctatc cgctgcacgg
    26821 caacgacatc gaccgcgaca cgctgcccgc cgaggcgggg cttcgctggg ccatcggcaa
    26881 ggacaaggac gccttcgccg gcaaggccgc gatcgaatcg aaagagccgg gcaagacgct
    26941 ggtcgggatc accatgaccg accgggtgat cccgcgccgc gggtgcgccg tcaccgccaa
    27001 cggccagacg gtggggactt gcaccagcgg cacgttttcg cccacgctca agatcgggat
    27061 cgccctggcg tcggtcgcgc cgggcagtgt caaggacggc gacgcggtga gcgtcgaggt
    27121 gcgcggcaag accggccagg gcatggtcaa gaagccaccg ttcgtggacg ccacgccccg
    27181 ctagcgcatc agcgcggcag gggagtgccc gacagcagcc agcgtgccac gaagatcgcg
    27241 gccgacagca ccagcgccac ggccgtcacg accgcgatca tcaccagcgt ctggg
//
LOCUS       DATLFO010000297        10650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_16815, whole genome shotgun sequence.
ACCESSION   DATLFO010000297 DATLFO010000000
VERSION     DATLFO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16815"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VM841_12085"
     CDS             <1..958
                     /locus_tag="VM841_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HVL90962.1"
                     /translation="AAHVAAMAPGTAIGAATPVDLQGGDISDKIINDAAAYAESIAQA
                     KGRNVTFAGEAVRKGRSVTAEEAARIDVVDLLADDVPDLLNAIDGQTLTLASGRDVTL
                     RTAGATMVPYELRGFRALLQRIADPNIAFLFLSIGTLAILYELGTAGVGGAGIVGVIM
                     IVLAFFSLSVLPVNAVGALLMFLAAILLIAEVFTPGVGVFAAGGVAALVLGGIFLFRG
                     SVGVDAGIIVPVALSVGAASVFIGRFAWRTRNRPSISGEAGLIGERGVVRSASDGRGR
                     ALIAGAWWNIRSDSPLTAGRTVRVKAVEGLDLIVEEEEEDKP"
     gene            955..1755
                     /locus_tag="VM841_12090"
     CDS             955..1755
                     /locus_tag="VM841_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVL90963.1"
                     /translation="MSDATLLGIVIGAVLLLSLFASAIRIVKEYERGVIFRLGRVIGA
                     KGPGLFFIIPIIDRMVKVTLQTVTLDIPPQDIITRDNVTVRVNAVAYFNVVDPIKAVV
                     QIQNYFFATSQISQTTLRSVVGQAELDDLLIKRDEINQQLQQIIDELTNPWGVKVTVV
                     EVKDVELPETMRRAMARQAEAERDRRAKIIHAEGEYGAAERLSAAAAELQKNPAALQL
                     RILSTMTEVASERNSTLIFPLPIELLRLIDVVTENIAKPASKRAVSKR"
     gene            1844..4144
                     /locus_tag="VM841_12095"
     CDS             1844..4144
                     /locus_tag="VM841_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVL90964.1"
                     /translation="MDISLPFLNHGRRLDIKYLARRSWFVIAVFEVVVLAMLLSSQVG
                     GAVASVQLPELPEMQPLAEDSRILDVKGRQIALLHGEEHRIIVEREQISNAAIYTVLA
                     AEDHRFFDHGGVNGTAMLRAALANVKEGEIEQGGSTITQQFVRNAFAEVGKERTMARK
                     LNEAWLATRLEKQMAKDEILLGYMNTIYFGRGAYGIEAAAREYFGKPARHLDIPEAAF
                     LAGIIKSPETLSDDLEAAKFRRDYVIGRLLDVGWTTPATAAAAIKTPLKVRPRSEPPV
                     RAAHFVEYVRRTLITPADRGGFGLTDRDIYTGGLTIHTTLNLDTQSAAERAVMSVLPN
                     ASDPEAALVSMATSGEIRALVGGRNFNSIEKARGFNYATQIVPGAGRSAGSTFKPFTL
                     LAYLRAGLSPRDSYRAPSKIKVPSCGSKRDPWEPGNYGNNSYGTMDVVKATELSVNTV
                     YAQMVARVGPESAMKAAHDAGITSPLLGVCSIALGPFGVTPLEMARSYATFAGRGMRP
                     DPIAVTKIVDRGGTVIAERKPTAKRTIEEKHADMMNAILEGVVRNGTGRPARISLTSA
                     GKTGTTEKKRDAWYVGYTPAPGLVTAVWMGYPPGPDGKVPIMTRLHGRSVSGGGFPGR
                     IWARFMGAALTGAADKGFEGVQLPPAKRTVERKQSPQTEAVAIVREAPSAVTAYAVDP
                     SPAAPPPQQPKKKRRRKSPSPDPDPEPEPSPSGSTGSTGQPSSSSSEATPGKSPSDPT
                     PASSPSSSNPGSSSPDPGTSRSPGAA"
     gene            complement(4185..5018)
                     /locus_tag="VM841_12100"
     CDS             complement(4185..5018)
                     /locus_tag="VM841_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90965.1"
                     /translation="MRPSNRFVACLAAFAGLLAACGGGEPRSASTPRPSGPSLIIEGD
                     RLCAELIRREAEYLKGSEIDVAGYVAIGDNTLAFFRRLMPPAGDARWTEGLQVRWEAI
                     DDARRRDGDVVGALWQFIGAARVAGLWLCGPDQAAVLDAAVADAAFSSTIPPEEYATR
                     LDGVIAKYAPEHEEYHHDPDPEELKKRLEPGDEHSHGPGYLTIQSAMIDAMAALPGPA
                     GQERQAAIFLYFMRQSLAAETAGIRTGGVRGQALFEPSYAYTHQAIGVACLVWKLNLC
                     G"
     gene            complement(5038..5817)
                     /locus_tag="VM841_12105"
     CDS             complement(5038..5817)
                     /locus_tag="VM841_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90966.1"
                     /translation="MSPDGRFDAGMLVQDIVYAGLEALRSAVALDLAAYLHEAPDQGP
                     QLFLGAPTLAEIEPTAAFNLFAALRDGLGEERNGDDIVVPGFCAVAITTSGPVSRGVH
                     VLGRRDAALADYEREVLVRLAYTFAALAHSVEHAPPPDVSPVQAGAWFTPARVVVETV
                     PGGAEATVTVVAGDESRTQTAQASSTPRAVAVAAVAAVDPSLKVVEAGEEQIGDVRAV
                     LALVADATGRQTIGSAVVDEQNDSLRAAAAAALDAAVRLMV"
     gene            complement(5814..6674)
                     /locus_tag="VM841_12110"
     CDS             complement(5814..6674)
                     /locus_tag="VM841_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90967.1"
                     /translation="MGGRLERQIRLVPNVLAVSIGRERVVVLTDPATDAATLARVVTD
                     VCDAAGWAGRVEIVGGLRRARRPVRLPVAIPRRWTMVAGPLAAAMAMMVATLAGGVSS
                     PRMESPSEAPRTDPRPHAAAAPPAFAPAAEPPAAAAAGPALVPADAPPIAALPRLPKR
                     PGLARVKPLLSLGRPPAGPAVPSTPANPGPPPSQQPSQPGAAAPKPELDPKPQPRHPT
                     PSQAPPKPVASDRDDDRDDDGDDLGDDEHDDAAEGKNKKSGKTKTAGHSEDDNKPEHA
                     AEHGARDRDR"
     gene            6812..7642
                     /locus_tag="VM841_12115"
     CDS             6812..7642
                     /locus_tag="VM841_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90968.1"
                     /translation="MRVVRLLAAMVLAASVASPLSPASAHCPQHMQHCAPGIVPPGAN
                     ETTCESPGKPPVVLVHGTYGDMSGFWNVYIPALREHGYCVWVLDYGHRGTDDMETSVS
                     QITAFVTLTVLRYSSTGTVSLIGHSQGGLQIRHMLRHDPSIPVQDAISLSGSHFGTDT
                     PLAPGAAEHGACPACAQQIQGSEWMTQHMNTGDLTPGAASYTQIETIYDEIVTPYTNT
                     LLPEEPGVVTNVVLQDRCPHDAFEHVLLTHDPFALAWALNALGTDGPADPAFVPTGCL
                     "
     gene            complement(7639..8448)
                     /locus_tag="VM841_12120"
     CDS             complement(7639..8448)
                     /locus_tag="VM841_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL90969.1"
                     /translation="MLSRTSEYARFHVTYESDGLTISGVMNVPSGPGPFPVLILNHGY
                     IDPGVYVNGQGMRREQDYLARRGYVVVHIDYRNHAESDRDPNADLELRLGYVRDSINA
                     LDAMRSSDLPFADTGRAAMIGRSMGGSVTMRAAVTHPDLFDAYVLFAPTSSDAVDNFD
                     KWTRGRSDRRSLAARIIAAYGSPESNPRFWDDVSPVTFVDRVAAPILIHHGTRDDTCS
                     IEWSERTAAALEAAGKDVQLLRYDDGHAFGPAFPESMRRTVDFLDRHVKTG"
     gene            8914..>10650
                     /locus_tag="VM841_12125"
     CDS             8914..>10650
                     /locus_tag="VM841_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90970.1"
                     /translation="MNRHPEHEAEQAYIERAYQRLDDLRAMALERAAEHARETTRMFG
                     QGYDRDVSVGVALKRAAELDIGDESIIFGRIDAETGERYYIGRRNVADEDNTPLVIDW
                     RVPAAEPFYRATGQEPLGLTMRRHFLCQHRTLLALEDEHFGEGTDLGMAGTGALLAAL
                     ERPRTGTMRDIASTIRAEQDRIIRAPLEGILAIQGGPGTGKTAVALHRAAYLLFTHRR
                     RLGQNGILFVGPNRLFLRYVGRVLPALGESGALMVMPEDLVEGIRVRATDEPAAARVK
                     GDVRMIRVLEAAIADIQRRRVPDISFSFEGYDITLPGRDIVPVVKSLRRGRGTHNARH
                     ASLRRRLSARLYDAYRRVLQDREGRNASLILLPRKAGEQAIRADESFDAIADSIWPLL
                     TPAALVGTLLSSPDALRSAARGILSAGEIESILRHEPDAWTAGDIALLDEARSVLGAP
                     IRDRNPDAEDDLDEIETFAHVVADEVQDLTPMALRMLGRRARHASMTIVGDLAQSVGA
                     LPPRSWSEIIAHLDPRRGSAVEELTINYRTPGSIMDVAVRVLALAEPGIVPPTSVRDG
                     GEPVEILAGPVVA"
BASE COUNT         1632 a         3715 c         3784 g         1519 t
ORIGIN      
        1 ggcggcgcac gtcgcggcga tggcgcccgg aacggcgatc ggcgcggcga cgccggtcga
       61 tcttcagggc ggggatatca gcgacaagat catcaacgac gcggccgcct acgccgagtc
      121 gatcgcccag gccaagggcc gcaacgtcac cttcgcgggc gaggccgttc gcaaaggccg
      181 cagcgtgacc gccgaggagg ccgcgcgcat cgacgtggtg gacctgctcg ccgacgacgt
      241 gccggatctg ctcaacgcga tcgacggcca aacgctcacg ctggcctcgg gccgggacgt
      301 gaccctgcgc accgcgggag cgacgatggt cccgtacgag cttcgcggct ttcgcgcgct
      361 gctgcaacgg atcgcggacc cgaacatcgc gttcctgttc ctttccatcg gcacgctcgc
      421 gatcctctac gagttgggga ccgccggggt cggcggtgcc ggcatcgtgg gcgtgatcat
      481 gatcgtgctc gcgttcttca gcttgtcggt gctgccggtc aacgcggtcg gagcgctgct
      541 gatgttcctc gccgcgatcc tgctgatcgc cgaggtgttc acgccggggg tgggggtgtt
      601 cgctgccggc ggggtggcgg cgctcgtgct cggtggcatc ttcctgttcc gcggctcggt
      661 cggcgtggac gcggggatca tcgtgccggt ggcgctgtcg gtgggagccg cctccgtgtt
      721 catcggccgc ttcgcgtgga ggacccgcaa ccgtccctcg atcagcggcg aggcgggcct
      781 gatcggcgag cggggcgtcg tccgcagcgc ctcggacggc aggggccggg cgctcatcgc
      841 gggtgcttgg tggaacatcc gctccgattc gccgctcacg gcgggacgga ccgtgcgggt
      901 gaaggcggtc gaggggctcg acctgatcgt ggaagaggaa gaggaggaca agccatgagt
      961 gatgcgaccc tgctcggcat cgtgatcggc gcggtgctgc tgctgtcgct tttcgcgtcg
     1021 gcgatccgga tcgtcaaaga atacgagcgg ggcgtgatct tccggctcgg acgggtcatc
     1081 ggggccaagg gaccgggcct gttcttcatc atcccgatca tcgaccgcat ggtgaaggtc
     1141 actctgcaga cggtgaccct ggacatccct ccgcaggaca tcatcacgcg cgacaacgtg
     1201 acggtccggg tcaatgcggt cgcgtacttc aacgtcgtgg acccgatcaa ggccgtcgtc
     1261 cagatccaga actacttttt cgcgacctca cagatctcgc agacgacgct gcgaagcgtg
     1321 gtcgggcagg ccgagctgga cgacctgctg atcaagcggg acgagatcaa ccagcagctt
     1381 cagcagatca tcgacgagtt gaccaatccc tggggcgtga aggtcacggt cgtggaggtc
     1441 aaggacgtcg agcttcccga aacgatgcgc cgcgccatgg cacgtcaggc cgaagccgag
     1501 cgcgaccggc gggccaagat catccacgcc gaaggcgagt acggggcggc cgagcggctg
     1561 agcgcggcgg cggcggagct tcaaaagaac ccggcggcgc tgcagctccg gatcctgtcg
     1621 acgatgacgg aggttgcgtc ggagcggaac tcgacgctga tcttcccgtt accgatcgag
     1681 ctgctgcggc tgatcgacgt ggtgacggag aacatcgcga agccggcctc gaagagggcg
     1741 gtatccaaaa gataaccatc cggcctgact acgaaacatc gggcgttggc gggggcaggg
     1801 tgcacgctta acgtggactc cgtcggggaa tacctgttcc acgatggata tctcactccc
     1861 gttcctgaat cacggccgcc gtctggacat caagtacctc gcgcgccggt cgtggttcgt
     1921 catcgccgtc ttcgaagtcg tcgtcctggc gatgctgctt tccagccagg tcggtggcgc
     1981 cgtcgcgagc gtccagctgc ccgagctacc cgagatgcag ccgctggccg aggactcgcg
     2041 gatccttgac gtgaagggcc ggcagatcgc cctgcttcac ggcgaggagc accgaatcat
     2101 cgtcgagcgg gagcagatct ccaacgccgc gatctacacc gtcctggccg ccgaggatca
     2161 ccggttcttc gaccacgggg gcgtgaacgg cacggccatg ctccgggcgg cgctcgccaa
     2221 cgtgaaggag ggcgagatcg agcagggcgg ctcgaccatc acccagcagt tcgtccgcaa
     2281 cgccttcgcc gaggtcggca aggagcggac catggcccgc aagctcaacg aagcctggct
     2341 cgcgacccgc ctcgagaagc agatggccaa ggacgagatc ctgctcgggt acatgaacac
     2401 gatctacttc ggccgcggcg cctacggcat cgaggccgcg gcccgcgagt acttcggcaa
     2461 gccggccagg cacctcgaca tccccgaggc cgcgttcctc gccggcatca tcaagtcacc
     2521 cgagacgctc tcggacgatc tcgaggccgc caagttccgc cgcgattacg tcatcggccg
     2581 gctgctcgac gtgggctgga cgaccccggc gacggccgcg gccgcgatca agacgccgct
     2641 gaaggtccgt ccgcgctccg agccgcccgt ccgcgccgct cacttcgtcg agtacgtgcg
     2701 ccgcacgctg atcaccccgg ccgatcgcgg tggcttcggg ctcaccgacc gggacatcta
     2761 caccggcggg ctgaccatcc acaccacgct taacctcgac acgcagtcgg ccgcggagcg
     2821 cgccgtcatg tccgtgctgc cgaacgcaag cgaccccgag gccgccctcg tctcgatggc
     2881 gacctccggc gagatccggg cgctggtcgg cggccggaac ttcaacagca tcgagaaggc
     2941 gcgcggcttc aactacgcga cgcagatcgt gccgggcgcg ggacgaagcg cgggctcgac
     3001 gttcaagccg ttcaccctcc tggcctacct gcgcgccggc ctatcgccgc gcgacagcta
     3061 ccgggcgccg tccaagatca aggtcccctc gtgcggcagc aagcgcgacc cgtgggagcc
     3121 gggcaactac ggcaacaact cctacggcac gatggacgtg gtcaaggcga cggagctttc
     3181 ggtgaacacg gtctacgcgc agatggtcgc tcgcgtcggc cccgagtcgg cgatgaaggc
     3241 cgcgcacgac gccggcatca ccagcccgct cttgggcgtg tgctcgatcg cgctgggtcc
     3301 gttcggcgtc acgccgctcg agatggcccg ctcctacgcg accttcgccg gccgagggat
     3361 gcgccccgac ccgatcgcgg tgaccaagat cgtggatcgc ggcggcacgg tgatcgccga
     3421 gcgcaagccg accgccaagc gcaccatcga ggagaagcac gccgacatga tgaatgccat
     3481 cctcgagggc gtcgttcgca acggtaccgg gcggccggct cgcatctcgc tgacctcggc
     3541 cggcaagacc ggaacgaccg agaagaaacg cgacgcctgg tacgtcggct acacgccggc
     3601 gcccggcttg gtgacggccg tgtggatggg ttatccgccg ggaccggacg gcaaggtgcc
     3661 gatcatgacg aggctgcacg gccgcagcgt ctcgggcggc ggcttccccg gccggatctg
     3721 ggcgaggttc atgggcgcgg cactgaccgg agcagccgac aagggcttcg agggtgtgca
     3781 gctccctccg gccaagcgca cggtcgagcg caagcagtcc ccgcagaccg aagccgttgc
     3841 catcgtgcgc gaggccccca gcgccgtgac cgcctacgcg gtggacccga gcccggccgc
     3901 gccgccgccg cagcagccga agaagaagcg tcgccgcaag agcccgtccc cggatccgga
     3961 tcccgagccc gaaccttccc cgtcgggctc gaccggctcg accggccagc cgtcgtcgtc
     4021 gtcgagcgag gcgacgccgg ggaaatcgcc gagcgatccg acgcctgcgt cctcgccgag
     4081 ctcgtcgaac ccgggctcat cctcgccgga ccccggcacc tcgcggtcgc cgggcgctgc
     4141 ctagcgagcg cctcgccggt tcccgctggt catgcccgcc ggggtcagcc gcacaggttg
     4201 agcttccaca ccaggcaagc cacgccgatc gcttgatgcg tataggcgta gctgggctcg
     4261 aacagcgctt gcccgcgcac accgccggtg cggattcctg cggtctcggc ggccagtgac
     4321 tgccgcatga agtacaggaa gatcgcggcc tgacgctcct ggccggccgg gccgggaaga
     4381 gccgccatcg cgtcgatcat ggcggactgg atggtcaagt agcccggccc gtgggagtgc
     4441 tcgtcgcccg gctcgagccg cttcttcagc tcctcgggat cgggatcgtg gtgatactcc
     4501 tcgtgctccg gcgcgtactt cgcgatgacg ccgtcgagac gcgtggcgta ctcctcgggc
     4561 gggatcgtcg aggagaaggc cgcatcggcg acggcggcgt cgagcacggc ggcctgatcg
     4621 ggtccgcaca gccaaaggcc ggcaacgcgc gccgcgccga tgaactgcca gagcgcaccg
     4681 acgacgtcgc cgtcgcgccg gcgggcgtca tcgatggcct cccagcggac ctggaggccc
     4741 tcggtccacc gcgcgtcgcc ggcgggcggc atcagacggc gaaagaaggc aagcgtgttg
     4801 tcgccgatcg cgacgtagcc ggccacgtcg atctcgcttc ccttcagata ctctgcctcg
     4861 cggcggatca gctccgcgca caggcggtcg ccctcgatga tgagcgacgg gccgctaggc
     4921 cgcggcgtgg acgccgagcg cggctcgccg ccgccgcagg cggccagcag gccggcgaag
     4981 gcggccagac acgcgacgaa acgattcgat gggcgcacgg cgaccaaagg taccgcgtta
     5041 gaccataagc cgcacggcgg catcgagcgc agcggccgcc gccgcgcgaa gcgagtcgtt
     5101 ttgctcgtcc accacggccg agccgatcgt ctgacggccg gtcgcgtccg cgaccagagc
     5161 gagcacggca cggacgtcgc cgatctgctc ctcccccgcc tcgacgacct tcagggacgg
     5221 gtcgaccgcg gcgaccgccg cgacggcgac ggcacgcggc gtcgaggatg cctgcgcggt
     5281 ctgggtgcgg ctttcgtcgc cggcgacgac cgtgacggtt gcttcggcgc ccccggggac
     5341 cgtctcgacc acgacccgcg ctggcgtgaa ccacgcccct gcctgaacgg gcgagacatc
     5401 gggtggcggc gcatgctcga cggagtgcgc cagcgccgcg aacgtgtatg cgagccgcac
     5461 gagcacctca cgctcgtagt cggcgagcgc ggcgtcgcgc cggccgagca cgtgcacgcc
     5521 gcgcgagacc ggcccgctcg tggtgatcgc gacggcgcag aatcccggga cgacgatgtc
     5581 gtcgccgttg cgctcctccc caaggccgtc gcggagggcg gcgaagaggt tgaacgccgc
     5641 ggtgggctcg atctcggcga gcgtcggtgc gccgaggaac agctgcgggc cctgatcggg
     5701 agcctcgtgc aggtacgcgg ccaggtcgag ggcgaccgcg gaccgcagcg cctccaggcc
     5761 tgcgtagacg atgtcctgaa cgagcatgcc ggcgtcgaag cggccgtcgg ggctcaccgg
     5821 tcgcggtccc tggcgccgtg ctcggcggca tgctcgggct tgttgtcgtc ctcggagtgg
     5881 ccggccgtct tggtcttccc gcttttcttg ttcttgcctt cggccgcgtc gtcgtgctcg
     5941 tcgtcgccca agtcgtcacc gtcgtcgtcg cggtcgtcgt cgcggtcgga ggcgacgggc
     6001 ttgggcggag cctgagaggg agtgggatgc cggggctgcg gcttcgggtc gagctcgggc
     6061 ttcggcgccg cggcgccggg ctgcgacggc tgctgcgagg gcggcggccc ggggtttgcg
     6121 ggcgtggacg gaacggccgg gccagccggc ggacgaccca gcgagagcag gggcttgacc
     6181 cgcgcgaggc cgggacgctt cggaaggcgg ggcagcgccg cgatcggcgg cgcgtccgcc
     6241 gggacgagcg cggggcccgc cgcggccgct gcgggaggct cggcggcggg cgcgaaggcc
     6301 ggcggcgcag ccgcggcatg cggccgcgga tcggtgcgag gggcctccga cggcgactcc
     6361 atgcgcggcg acgacacccc gccggccagc gtcgcgacca tcatcgccat ggcggcggcg
     6421 agggggccgg cgaccatcgt ccagcggcgc gggatcgcga ccggcaggcg caccgggcgg
     6481 cgagcacggc gcaggcctcc gacgatctcg acgcgccccg cccacccggc cgcgtcgcac
     6541 acgtcggtca cgacgcgcgc cagcgtggcc gcgtcggtgg cgggatcggt cagcacgacg
     6601 acgcgctcgc ggccgatcga gaccgcaagc acgttcggca ccagccgaat ctgccgctcc
     6661 aggcgtcccc ccattgcggc cccgactgta tccaacgagc ccttccgaac cccggaacgc
     6721 gggacggcga cgcgatcgaa ccgcgcgcgc aaggagggat cggctcggcc cgcagcgaac
     6781 acagccccca caccgaacgg gaggaaatct tatgcgcgtg gtccgcttgc tcgcggcgat
     6841 ggtgctcgcg gcgtcggtcg ccagcccgct ttcgccggca tccgctcact gtccgcagca
     6901 catgcagcac tgcgcgcccg gcatcgtccc gccgggagcc aacgaaacga cctgcgagtc
     6961 gcccggcaag cccccggtcg tgctcgtgca cggcacctac ggcgacatga gcggattctg
     7021 gaacgtctac atccctgcgc tgcgcgagca cgggtactgc gtctgggttc tcgactacgg
     7081 acaccgcggg accgacgaca tggaaacgtc ggtgagccag atcaccgcgt tcgtgacgct
     7141 cacggtcctg cggtactcat cgacgggcac ggtctcgctc atcggccatt cgcagggagg
     7201 cctgcagatc cgccacatgc tccgtcacga tccgtcgatt cccgtccagg acgcgatctc
     7261 gctgtccggc tcgcacttcg gcaccgacac gccccttgcg cccggcgcgg ccgagcacgg
     7321 cgcctgcccc gcttgcgccc agcagatcca gggcagcgaa tggatgaccc agcacatgaa
     7381 caccggagac ctgacgccgg gggcggcctc ctacacccag atcgagacga tctacgacga
     7441 gatcgtcacg ccctatacca acacgctgct gcccgaggag ccgggcgtgg tgacgaatgt
     7501 cgtgctgcaa gatcgctgcc cgcacgacgc cttcgagcac gtgctgctca cccacgaccc
     7561 gttcgcgctc gcgtgggcgc tcaacgcgct gggcaccgac ggacccgccg acccggcctt
     7621 cgttccgacc ggctgccttt agcccgtctt gacgtgccgg tccaaaaagt ccacggtccg
     7681 gcgcatcgac tcgggaaagg cgggaccgaa ggcgtgcccg tcgtcgtagc gaagcagctg
     7741 cacgtctttg ccggcggctt cgagcgcggc ggcggtgcgc tcggaccatt cgatcgaaca
     7801 ggtgtcgtcg cgcgtgccgt gatggatcag gatcggcgcg gcgacccgat cgacgaaggt
     7861 gacgggtgag acgtcgtccc agaaccgggg attggactcg ggcgatccgt acgccgcgat
     7921 gatgcgcgcg gccagcgagc gccggtccga gcggccccgc gtccacttgt cgaagttgtc
     7981 caccgcgtcg gaggacgtgg gcgcgaacag cacgtaggca tcgaacaggt ccggatgggt
     8041 gacggctgct ctcatggtca cgctgccgcc catcgaccgg ccgatcatcg cggcgcggcc
     8101 ggtgtcggcg aagggcaggt ccgacgagcg catcgcgtcg agcgcgttga tcgaatcccg
     8161 aacgtatccg agccgcagtt cgagatccgc gttcggatcc cggtcggact ccgcgtggtt
     8221 gcgatagtcg atgtgcacca ccacgtagcc gcgccgcgcg agatagtcct gctcccgtcg
     8281 catgccctgg ccgttgacgt agacgccggg atcgatgtag ccgtggttga ggatgagcac
     8341 gggaaatggc cccggtccgc tcgggacgtt catcactccg gagatggtca agccgtcgct
     8401 ttcgtaggtg acgtggaagc gcgcgtactc cgaggtgcgc gacagcacgc ggccggtgcg
     8461 gaagtcgcgg ccggccggcg cgtcggcggc gagcgccggc agcgagaccg gatgcggttg
     8521 cggactcctc gcggcggagg gcgaggccgg cggcggcgcg ctcgtcgccg acggcgtggc
     8581 cgcgggcgcg ccggcggcgg cgcgctcgtc gccgacggcg tggccgcggg cgcggttgcg
     8641 tcgcgctcgg ggccggagtc gtccgcgagc gtgatcgccc cgacggcggc gccggcgatc
     8701 agcagcgcac cgagcgcggc ccggacggct ttgcgggcgt tcgcagccat cgcctcgaag
     8761 cctacgacgc ctccccggat cgtgccgggg gtgatcgcgg acgagcgcac ggcctgcctt
     8821 tcctcgaaga gccgggccat ctttggcaaa ccgcgcgcac tcgggcccgg aacctggaat
     8881 gatgaacggc tttcatcgat cggaaggaat cgagtgaacc gccaccccga gcacgaagcg
     8941 gagcaggcat atatcgagcg cgcctaccag cggctcgacg atctgcgtgc gatggcgctg
     9001 gagcgcgcgg ccgagcacgc ccgcgagacc acgcgcatgt tcgggcaggg ctacgaccgg
     9061 gacgtctcgg tgggcgtggc gctcaagcga gcggcggagc tcgacatcgg cgacgagtcg
     9121 atcatcttcg gccgcatcga cgccgagacc ggcgagcgct actacatcgg ccgccgcaac
     9181 gtggccgacg aggacaacac gccgctggtg atcgactggc gggttcccgc ggccgagccg
     9241 ttctaccggg cgaccgggca ggagccgctc gggctgacga tgcggcgtca cttcctgtgc
     9301 cagcaccgga cgctgctcgc cctggaggac gagcatttcg gggagggaac cgacctcggc
     9361 atggccggga cgggagcgct gctcgcggcg ctggagcggc cccgaaccgg aaccatgcgc
     9421 gacatcgcgt ccacgatccg ggccgagcag gaccggatca tccgcgcccc actggagggc
     9481 atcctggcga tccagggcgg accgggaacc ggcaagacag cggttgcgct ccaccgggct
     9541 gcttatctgc tgttcaccca ccgccgccgg ctcggccaga acggcatcct gttcgtcggt
     9601 cccaaccggc tgttcctgcg atacgtcggc cgcgtgcttc ctgcgctcgg cgagtcgggc
     9661 gcgctgatgg tgatgccgga ggatctcgtc gagggcatcc gcgtccgcgc gaccgacgag
     9721 cccgcggcgg cgcgcgtcaa gggggacgtg cgcatgatcc gcgtcctgga ggcggccatc
     9781 gccgacatcc agcgccgccg ggtgcccgac atctcgtttt cgttcgaggg atacgacatc
     9841 acgcttcccg gccgcgacat cgtgccggtg gtgaagtcgc tgcgccgcgg acgaggcacc
     9901 cacaacgcgc ggcacgcgag cttgcggcgc cggctctcgg cgcggctcta cgatgcgtat
     9961 cgccgcgtcc tgcaggaccg ggagggccgc aacgcgtcac tgatcctgct tccccgcaag
    10021 gcgggcgagc aggcgattcg cgccgacgaa tccttcgacg cgatcgccga ctcgatctgg
    10081 ccgctgctca cgcccgcggc gctggtggga acgctgctct cctctcccga tgcccttcga
    10141 tcagcggcac gcggcatcct gtcggccggc gagatcgagt cgatcctccg ccacgagccc
    10201 gatgcgtgga cggcgggcga catcgcactg ctcgacgagg cgcgctcggt gctcggcgcg
    10261 ccgatccgcg accggaaccc ggacgccgag gacgacctcg acgagatcga gaccttcgcg
    10321 cacgtagtcg ccgacgaggt gcaggacctg acgccgatgg cgctgcgcat gctcgggcgg
    10381 cgtgcccgcc acgcgtcgat gacgatcgtg ggcgatctgg cgcaatcggt cggcgcgctg
    10441 ccgccgcgct cctggagcga gatcatcgcg cacctggatc cgcggcgcgg atcggcggtc
    10501 gaggagctca cgatcaacta ccgcacaccc ggctcgatca tggacgtcgc ggtccgcgtg
    10561 ctcgcgctcg ccgagcccgg catagtgccg cccacgtcgg tgcgcgacgg cggcgagccg
    10621 gtcgagatcc ttgccgggcc ggtcgtggcc
//
LOCUS       DATLFO010000298         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_16890, whole genome shotgun sequence.
ACCESSION   DATLFO010000298 DATLFO010000000
VERSION     DATLFO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16890"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2532)
                     /locus_tag="VM841_12130"
     CDS             complement(<1..2532)
                     /locus_tag="VM841_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HVL90971.1"
                     /translation="MPAEEREKNTRAARINPRLTRSRWNAVSFAGPDVGKYVEAAVEE
                     FPTGAGPADYALCDGGDVLGVVEAKRVSLGPQNVLSQAERYSRGFDASPADYGDGFRV
                     PFLYSTNGEVIWFHDIRHALSRSRKVAQFHTPAALREMLGRDDSAVLDRLAGLPYHGL
                     LRDYQIEANEAVEDELRKRRRRMLLAMATGTGKTLTMVSEIHRLMKAGVARRVLFLVD
                     RRALAAQAVRTFASFEAEPGLKFDRIYEVYSQRFQRDDLDDLPFDPRILPASYLTDPQ
                     PGHAFVYVCTIQRMTMNLFGRDRALTPDDEPPDQDAEQVDIPIHAFDVVVADECHRGY
                     TARDISTWRNTLDYFDAVKIGLTATPAAHTVAYFDRPTYRYSYERAVREGHLVDYDAV
                     RVNSGVRVNGVFLHQGEHVGVIDPHTGTEDFDLLEDERQYVSEEVERTVTSPDSNRKI
                     LEEVRKYTDAHEVQFGRFPKTLIFAANDLPHISHADQLVDSARDVLGRGDAFVAKITG
                     KVDRPLQRIREFRNRPNPGVAVTVHLLSTGVDIPDLEFIVLLRQIRSRILFEQVLGRG
                     TRKGERFPDKSHFTVFDCFDGTLLEYFRNATGITAEPPEADPKIIAQMISDIWQNVDR
                     DHNLSRLVKRLRRIDKQMSGDARDQFTRFLPRGDLGAFAQALPTLLASAFTDTMDLLR
                     DEQFQDLLMNYPRPPRMFVVAYEVQDNVSSEWLIRGPSGQEFRPTDYLAAFEKFVADN
                     ADTIDAIAILLARPAGWGTDALHQLREALAGAAEQFTEANLQRAYEITHRKSLADIIS
                     MVKRAAGRMSDLLTADERVTQALERVVAGRQLSADQAKWLDYIRQH"
BASE COUNT          434 a          807 c          886 g          485 t
ORIGIN      
        1 gtgctggcga atgtaatcga gccacttcgc ctggtcggct gagagctgac ggccggcgac
       61 gactctttcc aacgcctggg tgacccgctc gtcggcggtg aggaggtccg acatcctccc
      121 ggcggcgcgc ttgaccatcg agatgatgtc agcgagtgac ttgcggtgcg tgatctcgta
      181 cgcgcgttgc aggtttgcct ccgtgaactg ctcggccgcc cccgccagcg cttcacggag
      241 ttggtgcagg gcgtcggtgc cccagccggc cggccgtgcg agcagaatgg cgatcgcgtc
      301 gatggtgtcg gcgttgtcgg ccacgaactt ctcgaacgcg gcaaggtagt cggtggggcg
      361 gaactcctgt ccggacgggc cacggatgag ccactcagag gacacgttgt cttgtacctc
      421 gtacgcgacc acgaacattc gcggcggcct cgggtagttc ataagcaggt cctggaactg
      481 ctcgtcgcgc aggaggtcca tcgtgtcggt gaacgcgctg gcgagcagcg tcggtagcgc
      541 ctgggcgaac gcgccaagat caccgcgagg taggaaccgc gtaaactgat cgcgggcatc
      601 gccggacatt tgcttgtcga ttcggcggag ccgtttaacg agacgactga ggttgtggtc
      661 gcgatcgacg ttctgccaga tgtcgctgat catctgggcg attatcttcg gatcagcttc
      721 gggtggttcg gcggtgatgc cggtcgcgtt gcggaagtac tcgaggagcg tgccgtcgaa
      781 gcagtcgaac acagtgaagt gcgacttgtc ggggaaccgc tcccctttgc gggttccgcg
      841 acccaagacc tgctcgaaca ggatgcgcga tctgatctgc cgcagcagca cgatgaactc
      901 aaggtcggga atgtccacgc cggtcgagag gaggtgaacg gtaacggcta cgccggggtt
      961 cggtcggtta cggaattcgc gtatccgctg gagcggccga tcgaccttgc ccgtgatctt
     1021 cgccacgaac gcatcaccac ggcctaacac gtcgcgagcg gagtcgacga gctggtcggc
     1081 atgcgaaatg tgcggcaggt cgttcgcggc gaagatcagg gtcttcggga accggccgaa
     1141 ctgcacctcg tgcgcatcgg tgtacttgcg gacctcttcg aggatcttcc ggttcgagtc
     1201 gggtgacgtg accgtgcgtt cgacctcctc ggagacgtat tggcgctcat cctccagtag
     1261 gtcgaagtcc tcggtgccgg tatgcgggtc gatcacccca acgtgttccc cctgatgcag
     1321 gaagacgccg ttgacccgga ccccagagtt gacccggacc gcgtcatagt caacgaggtg
     1381 cccttcgcgg accgcgcgtt cataggagta gcgatacgtg ggtcgatcga agtaggcgac
     1441 ggtgtgcgcg gccggcgtcg cggtcagccc gatcttgacc gcgtcgaaat agtcaagggt
     1501 gttgcgccag gtcgatatgt cgcgggccgt gtagccgcgg tgacactcgt ccgcgaccac
     1561 gacgtcaaag gcgtggatcg ggatgtcgac ttgctccgcg tcctggtccg gcggttcgtc
     1621 atctggagtg agcgcccggt cgcgaccgaa caggttcatc gtcatccgct ggatcgtgca
     1681 cacgtacacg aacgcgtggc cgggctgggg atcggtcagg taggaggcgg gcagaatgcg
     1741 ggggtcgaac ggcaggtcat cgaggtcgtc gcgctggaaa cgctggctgt agacctcgta
     1801 gatcctgtcg aacttgagac ccggctcggc ctcgaaggac gcgaacgtcc ggactgcctg
     1861 ggcggccagc gctcggcggt cgaccaggaa cagcacccgc cgggcaacac cggccttcat
     1921 caaccgatgg atctcgctga ccatcgtcag cgtcttgccg gtgccggtcg ccatagcgag
     1981 gagcatcctg cggcggcgct tccgcagctc atcctcgacc gcctcgttgg cctcgatctg
     2041 gtagtcgcgt agcagcccgt gatacggcaa tccagcaagc cggtcgagca cggcactgtc
     2101 gtcgcgcccg agcatttccc gcagcgcggc cggcgtgtgg aactgcgcga ctttccgcga
     2161 gcggcttagc gcgtggcgga tgtcgtggaa ccagatcacc tcgccgttgg tggagtacag
     2221 aaatggcact cggaacccgt ctccgtaatc ggcgggactg gcgtcgaagc cccgggagta
     2281 gcgctcggcc tggctcaaca cgttctgtgg acccaggctg accctcttgg cttcgacgac
     2341 gccgagcaca tcgccgccgt cacaaagggc gtagtcggcc ggcccggccc cagtggggaa
     2401 ctcctcgacc gccgcctcga catattttcc aacgtctggg ccggcgaatg agacggcgtt
     2461 ccaccggctg cgcgtcaaac gagggttgat ccgtgccgct cgggtgttct tctcgcgctc
     2521 ctccgcgggc atctgtaccg gtcctcgtgt cgccgtcgcc gagtgcgagg tcagccaccg
     2581 actgtaccgg cgccgacgcg ccgctgacgt cc
//
LOCUS       DATLFO010000299         7580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_38124, whole genome shotgun sequence.
ACCESSION   DATLFO010000299 DATLFO010000000
VERSION     DATLFO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38124"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VM841_12135"
     CDS             complement(<1..274)
                     /locus_tag="VM841_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90972.1"
                     /translation="MSSPNALIGRIIVLFRVRGIEIAFTPSWLATVVVLSLIFRQSDL
                     IPASAGRVGGAAMSVGLTLLFYSFVLMHEAAHTFVAHVFGLQPKRIL"
     gene            complement(316..723)
                     /locus_tag="VM841_12140"
     CDS             complement(316..723)
                     /locus_tag="VM841_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90973.1"
                     /translation="MRRPTRILIAAATLAAASAWPAAAAPDGFGQLAGNGTAAAAGCA
                     GPFTVTGISTTPGTWSFTVSFAGAGTAICAAGGAPVATGAWSPAAGGCVGGSTGLVCV
                     GKVLPTGTPVSVAVSFCIPAQACYSGTASVVRL"
     gene            complement(871..2679)
                     /locus_tag="VM841_12145"
     CDS             complement(871..2679)
                     /locus_tag="VM841_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HVL90974.1"
                     /translation="MPYTIAEEQVVLASALDDAALTPPGEIRTLVRLARSLRAVPDPE
                     IDPAFAARLEARLLTEGLDELSARRRPVLASAPKVAVSPVPHAAPVVALPRGRFVVRK
                     AVIGLIAAAMLFALPVVSAGSALPGSPLYGVKTAKERIELALARGPVDRGFVHLRHAK
                     QRLDEVEQLIVTRREGFVPSTLGRYDHAIDTGTHLILSATGDPAALARLAAEIRRGTG
                     SLSQLLDVASPVVAPAIIESIATGGKLSLRVRLALGDVGTNVPVTPAAALPDSAGRVT
                     AGSSPVVGGFGGAAPAPAARPEAPDAPIEEGPPGDDDEGPNSRGAFSSCLGMHVSDPT
                     GRAGSACGVAVSIRDLIGSGHDTRPGESAVSGCPAATTCGVYALDRHRWPTSAGIATL
                     SFVVNPVQPWVTPSEAVAAAVAAARAWSDANPMVRVDYRGTTTALPALGDGVNQIGWG
                     NPGATNAVAQANILRVGDRVIEADIVLTASLPWATSHSGAVKRYDIRSVLTHEFGHWL
                     GLDHLEIAAASEQSMFSRPRPGDARGATLALGDVRAIRVAYPCRGCAMPAISADDLGD
                     GDGDGDDAPPAPAAQEPSRIPAGDGRHARESAAGTT"
     gene            complement(2930..3754)
                     /locus_tag="VM841_12150"
     CDS             complement(2930..3754)
                     /locus_tag="VM841_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90975.1"
                     /translation="MLAAAVVAVCAMLPAYSNPDPFSDGVAKDEVSAASGCSAPLTGI
                     GGHGFNYCGDHWRLSKLPVRFRLNPSGTPSTVASNLALAADLAAAAWDSASPVSGGGP
                     RPNRCTAATRIICVEALDHSAVVNPTDGKNVIVWQNLGAAAAPAYADIRLSGHRIEDV
                     DIVLNGSLSWYWDDAALVATGVALGPVAPFCPRFACPLRYDLQAILTHEFGHALGLLH
                     VNPGSPAVWPSDLTDAPDYNLVMYPVYYPNNATWRVLGWGDLAGLNVAMRVSASDP"
     gene            complement(3798..3977)
                     /locus_tag="VM841_12155"
     CDS             complement(3798..3977)
                     /locus_tag="VM841_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90976.1"
                     /translation="MQPRKDNQKRITATHAMHDSLDATEAHHEIDLKMVEALQRIERV
                     RSKALAALGTLISSG"
     gene            complement(4135..4554)
                     /locus_tag="VM841_12160"
     CDS             complement(4135..4554)
                     /locus_tag="VM841_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90977.1"
                     /translation="MRKAPGLLLALALLLGVSPARAQTVQHAILLPDFGTPSLGRVLA
                     SEAGLNGVIGYLIAIQGTKTKFTLATVSAPTGVESFDIFFYDDSNGKPGTVIAAYNLC
                     PGGELRCDKSGSIPSRARWAVVTLVLGVNGTFRYVAS"
     gene            complement(4705..5781)
                     /locus_tag="VM841_12165"
     CDS             complement(4705..5781)
                     /locus_tag="VM841_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90978.1"
                     /translation="MKPTTDTTLALALALDGAAAPATDEIKRLSGIAGLVDALPDPEI
                     DSRFVARLEATLMAEYDAKFGTQAESSPAPLRIVKSEPQSKTEQKAEPKAAPNVVAFP
                     RRRMVVRRAVAGMVAAAMLSAMPVVAAASSLPGSPFFGIERWRQNHAISSAHGAEKAF
                     VSESIARKWIGYGSQMTVLDYDPSDIEAVLFRAGELQTQAADLIARHGTKEQIAEMAG
                     MFAQDATQLREILPKAAVSSRAAVEDAIATATGLTRTLVGKLRSLDATFAIPAAVEAT
                     ASKDGSAGSPKSTTPARGDAKSPDTGEEAPDKVADDTRREYAETAGDACEMIAREQAN
                     DLLAGPSKVACTVRDFDPDDWTKK"
     gene            complement(5778..6530)
                     /locus_tag="VM841_12170"
     CDS             complement(5778..6530)
                     /locus_tag="VM841_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL90979.1"
                     /translation="MKQAESQQSGDLSVSVSASSVTVQVTPAAPEAEQLVARAKEGDR
                     GAFAQLYRMYLPNVYKFLYYRVGSKAQAEDMTAEVFLRALRKIESFTWTGADFGAWLM
                     RIARNLVLDEAKSSRARLEVVSDEMPEERTGENKAAESAAMDNFTNVEVYKAIKKLRP
                     DQQEVITLRFLHGMGVGEVAKLMDKKEGTVRTLQFRGLKALEKILVARGVVDAPAALK
                     VRPEGVRAESNGRTGDRTEAEAARFGDKDGAR"
     gene            complement(6714..>7580)
                     /gene="speB"
                     /locus_tag="VM841_12175"
     CDS             complement(6714..>7580)
                     /gene="speB"
                     /locus_tag="VM841_12175"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HVL90980.1"
                     /translation="GIPDGARFLAADASYEEAVAVIQGLPWDGSVSWRAGARDAPSAV
                     RIASDSIETYSPVLRADLEDHAIFDAGDLALAGKAPEDAIDLIAAETERHLRAGKFLI
                     SVGGDHSVSIGTTRGARRVHPGLVHLVYDAHMDMRASYDGTDLSHACGTRHMAMAGTT
                     IALGIRSGAREEFTDADKMLAYWSTEVEIVPQVRSLLQGRPVFVSCDLDVLDPGQLPG
                     TGTPEPGGCSYKELRASLLSLQGLNVVGVDLVEVSPNWDPSGRSPVVAAQLSREIFLG
                     LGVVKILSDQGK"
BASE COUNT         1178 a         2607 c         2618 g         1177 t
ORIGIN      
        1 gcaggatacg cttgggctgc aggccgaaca cgtgcgcgac gaacgtgtgg gccgcctcgt
       61 gcatcagcac gaacgagtag aacagcaacg tcaggccgac cgacatcgcc gcgccgccga
      121 cccggccggc actcgccggg atcaggtccg actggcggaa gatcagcgac aggacgacca
      181 cggtcgcgag ccaggacggc gtgaaggcga tctcgatccc ccggacgcga aacagcacga
      241 tgatccggcc gatgagcgcg ttcggggagg acatgggccc caatgctacc ggcctcgacg
      301 acccggagcc gccggctaca gccggacgac ggaggcggtt cccgagtagc aggcctgagc
      361 cgggatgcag aaggagactg ccacgctgac gggcgtaccg gtcggaagga cctttccgac
      421 gcacaccaga ccggtcgagc cgcccacgca tccgccggcg gccgggctcc acgctcccgt
      481 cgccaccggc gcgccgccgg ccgcgcagat cgcggtgccg gcgccggcaa acgagaccgt
      541 gaacgaccac gtgcccggcg tggtcgagat ccccgtcacg gtgaacggtc cggcgcagcc
      601 ggccgcggcg gcggtgccgt tgccggcgag ctggccgaat ccgtccgggg cggcggccgc
      661 gggccaggcc gaagcagcgg ccagcgtcgc ggccgcgatc aaaatccgcg ttggtcgtct
      721 cacgtccgtg gttccctgat cccacggccg ggccgtctcg gcccgtcctc gccgctgggc
      781 atccccctca tcgttcggga cgccccgccg cgactccttc ttctgctgct gcttccttct
      841 tctgctgctg cttccgttct tctggtgctg ctacgtggtg cccgcagccg attcccgcgc
      901 atggcgtccg tcaccggcgg ggatccggga cggctcctga gcggccggcg ccggcggcgc
      961 atcgtcgccg tcgccgtcgc cgtcgcccag atcgtcggcc gagattgccg gcatcgcaca
     1021 gccacggcac gggtaggcaa cgcggatcgc tctgacgtcg cccagcgcga gggtggcgcc
     1081 ccgcgcatcc cccggacggg gccgcgagaa catcgactgc tcggacgcgg cggcgatctc
     1141 aagatggtcc agaccgagcc agtgtccgaa ctcgtgcgtg agcaccgatc ggatgtcgta
     1201 gcgcttgacc gcgccggagt gcgaggtcgc ccacggcagc gaggcagtga gcacgatgtc
     1261 tgcttcgatc acacgatcgc cgacccgcag gatgttcgct tgcgcgacgg cattggtcgc
     1321 acccgggttg ccccagccga tctggttcac gccatcgccg agcgcgggca gcgcggtggt
     1381 cgttccgcga tagtccacgc gcaccatcgg gttggcgtcc gaccaggcgc gggcggccgc
     1441 cacggccgcg gccacggcct ccgacggcgt cacccacggc tgcacgggat tgacgacgaa
     1501 cgacagcgtc gcgatgccgg cgctggtcgg ccagcgatgc cggtcgagcg cgtacacgcc
     1561 gcacgtcgtc gcggcggggc atccgctgac ggcggactcg ccgggccgcg tgtcgtgacc
     1621 ggatccgatc aggtcgcgga tcgagacggc gacgccgcag gcggagccgg cgcgtccggt
     1681 cggatcggac acgtgcatcc caaggcacga gctgaacgca ccacggctgt tcggcccttc
     1741 gtcgtcgtcg ccgggtggtc cctcctcgat cggcgcgtcc ggcgcctcgg gacgggcggc
     1801 gggcgccggc gcggcgccgc cgaacccgcc caccacgggg gacgacccgg ccgtgacgcg
     1861 gcccgcggag tcgggcagcg cggccgcggg cgtcaccggg acgttcgtgc cgacgtcgcc
     1921 gagcgcgaga cgcacgcgca gcgacagctt gccgccggtg gcgatcgact cgatgatcgc
     1981 cggcgccacc acgggcgatg ccacgtccag cagctgggaa agactgcccg ttccccggcg
     2041 aatctccgcg gcgagacggg cgagcgccgc aggatctccg gtggcggaca ggatcagatg
     2101 ggttccggtg tcgatcgcat ggtcgtagcg accgagcgtg gacggcacga aaccctcgcg
     2161 ccgggtcacg atgagctgct cgacctcgtc gagccgctgc ttcgcgtgac gcaggtgaac
     2221 gaagcctcgg tcgaccggcc cgcgcgcgag cgcgagctcg atccgttcct tcgccgtctt
     2281 gacgccgtag agcggcgagc cgggaagtgc gctgccggcc gacacgaccg gcagcgcgaa
     2341 cagcatcgcg gcggcgatca gtccgatcac cgccttgcga actacgaaac ggccgcgcgg
     2401 aagcgcgacc acgggtgctg cgtgtggaac cggcgatacc gcaaccttcg gcgccgaggc
     2461 caagaccggc cgcctgcgcg ccgaaagctc gtcgagccct tccgtcagca gtcgcgcctc
     2521 gagccgggcc gcgaatgccg gatcgatctc cgggtcgggc acggcgcgaa gggagcgtgc
     2581 gagacgaacg agggtgcgaa tctcgccggg cggggtgagg gcagcatcgt ccaacgcaga
     2641 cgcgagaacg acctgctctt cggcgatcgt atagggcatc tcctctcctc ctccgacaag
     2701 gaaaaacgag atgcctcgcc gcgtgttcta cttttttcga ccaggaaatg agttttcttc
     2761 gaataggcgc gtgacctgct gatatcagcc gatcggaaac gacgaagcgg accgcgatag
     2821 gtccgcttcg tcgttgttgc caccgattgc tcgaaggcgg gccgggcccg gtgggccgcg
     2881 ccaggtccgg tccttatcca cgggccgcgg cccgccctcg agcgtccggt cagggatcgg
     2941 atgccgacac gcgcatcgcg acgttgaggc cggcgagatc accccagccg agcacgcgcc
     3001 acgtagcgtt gttcgggtag tagaccggat acatcaccag gttgtaatcg ggcgcgtcgg
     3061 tcagatcgga cggccagacc gcaggcgagc cgggattgac gtggagcaag ccgagggcgt
     3121 gcccgaactc gtgggtgagg atcgcttgca gatcatagcg aagcgggcat gcgaagcgag
     3181 ggcagaacgg cgcgaccggc ccgagcgcga cgcccgtggc caccagcgcg gcgtcgtccc
     3241 agtaccagct cagcgacccg ttcagcacga tgtcgacgtc ctcgatgcga tgtcccgaca
     3301 ggcgtatgtc ggcgtaggcg ggcgccgcgg ccgctccgag gttctgccac acgatgacgt
     3361 tcttgccgtc cgtgggattc accaccgccg aatggtcgag cgcctcgacg cagatgatgc
     3421 gggtggccgc cgtgcagcgg ttcggtcgcg gcccgccgcc cgagacgggc gaggcgctat
     3481 cccaggccgc ggcggccaga tcggccgcga gcgccaggtt ggaagccacc gtcgaaggcg
     3541 tgccgctggg gttgaggcga aaacgcaccg gcagcttgct caaacgccag tgatcgccgc
     3601 agtagttgaa tccgtgaccg ccgatgccgg tcagcggggc gctgcatccc gacgcggcag
     3661 aaacctcgtc cttggcgacg ccgtcggaga acggatcggg attggagtac gcgggcagca
     3721 tcgcgcagac ggctacgacc gccgccgcga gcacgatccg tctgcttcgc gtgggactca
     3781 acccctccgc ccgtgtccta ccccgacgag atcagcgttc ccaaagccgc gagcgccttc
     3841 gagcgaaccc gttcgatccg ctgcagcgcc tcgaccatct tgagatcgat ctcgtgatgc
     3901 gcttccgttg cgtccaacga gtcatgcatc gcgtgcgttg cggtgatgcg cttctggttg
     3961 tccttcctcg gctgcatgat gctcgatcct ccctcgatgc gcccggcgcg gcgttcgcgg
     4021 cgatgtgctc cctctcgcct ggtaagaacg aactagtccc gacttcgttc cgggacgaaa
     4081 gccgcgatcc ggccggcggt cggggatgaa agggacggac gggcagggcg cccgtcagga
     4141 ggcgacatag cggaaggtgc cgttcacacc cagcaccagg gtcaccacgg cccagcgggc
     4201 ccgcgacggg atcgagccgc tcttgtcgca acgcagctcc ccgccggggc acaggttgta
     4261 ggcggcgatc accgtgccgg gcttgccgtt gctgtcgtcg tagaagaaga tgtcgaacga
     4321 ctcgacgccg gtgggcgcgg atacggtcgc gagcgtgaac ttcgtcttgg tgccttgaat
     4381 ggcgatgagg tagccgatga cgccgttcaa gcccgcctcg ctcgccagga cgcggccgag
     4441 cgacggcgtg ccgaagtcgg gcaacagaat cgcgtgctga accgtctgcg cgcgtgccgg
     4501 cgaaacgccc agcagcaatg cgagcgcgag gagcagcccc ggcgccttcc tcattcgatc
     4561 tccctcccga gccaacggga ttcctggtca gggaagattc gcccggtgat gctcgaaatc
     4621 ctgctatgcg cggggcgagc cggtcgaaga aaacgaaagc ggggacccgg agggtcccca
     4681 cttttcggtc gaaacgtccc tgcgttactt cttcgtccag tcgtccgggt cgaagtcgcg
     4741 caccgtgcaa gcgaccttgg acgggccggc cagcagatcg ttggcctgct cgcgagcgat
     4801 catctcgcac gcgtcgccgg cggtctccgc gtactcgcgg cgcgtgtcgt cggcgacctt
     4861 gtcgggtgcc tcctcgcccg tgtccgggct cttggcgtcg ccgcgcgcgg gcgtcgtgga
     4921 cttcggcgaa ccggccgagc cgtccttcga cgccgttgcc tcgacggccg ccgggatcgc
     4981 gaaggtcgcg tcgaggctgc gcagcttgcc caccagcgtg cgggtcaggc ccgtcgccgt
     5041 ggcgatcgcg tcctcgaccg ccgcccgcga cgacaccgcg gccttcggca gaatctcacg
     5101 aagctgcgtg gcgtcctgcg cgaacatgcc ggccatctcg gcgatctgct ccttcgtgcc
     5161 gtgacgggcg atcaggtcgg ccgcctgcgt ctgcagctcg ccggcccgga acagcaccgc
     5221 ctcgatgtcg ctcgggtcgt agtcgagtac cgtcatctgc gagccgtacc cgatccactt
     5281 gcgtgcgatc gactcggaca cgaacgcctt ctcggcgccg tgcgcggagc tgatcgcgtg
     5341 gttctgccgc cagcgttcga tcccgaagaa cgggctgccc ggcagcgacg aggccgccgc
     5401 aaccaccggc attgccgaga gcatcgccgc ggcgaccatg cccgcgacgg cgcggcgaac
     5461 caccatgcgg cggcgcggga aggccaccac gttcggcgcg gccttgggct ccgccttctg
     5521 ttcggtcttg ctctgcggct cggacttgac gatgcgcagc ggcgcgggcg acgactccgc
     5581 ctgcgtgccg aacttggcgt cgtactcggc catcagcgtt gcttcgaggc gcgcaacgaa
     5641 gcgcgagtcg atctcgggat cgggcagtgc gtcgaccaag ccggcgattc ccgagagacg
     5701 cttgatctcg tcggttgccg gcgcggccgc gccgtccagc gcgagcgcca gagcgagggt
     5761 cgtgtcggtc gtcggcttca tcgggctccg tccttgtctc cgaatcgagc ggcttcggct
     5821 tcggtccggt cgccggtgcg accgttggat tccgctcgga cgccttctgg gcgtaccttc
     5881 agagcggccg gcgcgtcgac gacgccccgg gccaccagga tcttctcgag ggccttgagt
     5941 ccgcggaact gcaacgtgcg gaccgtgcct tccttcttgt ccatcagctt ggccacctcg
     6001 ccgacgccca tgccgtgcag gaagcgaagg gtgatcactt cctgctggtc gggacgcagc
     6061 ttcttgatgg ccttgtagac ctcgacgttg gtgaagttgt ccatcgcggc ggactcggcg
     6121 gccttgtttt cgccggtgcg ctcttcgggc atctcgtcgc tgaccacttc caggcgggcc
     6181 cgcgaggact ttgcttcatc gaggacgagg ttgcgcgcga tgcgcatcag ccaggcaccg
     6241 aagtcggcgc cggtccaggt gaaggactcg atcttgcgca gcgcgcggag gaagacctct
     6301 gcggtcatgt cctcggcctg cgccttgctg cccacgcggt agtacaggaa cttgtacacg
     6361 ttgggcagat acatgcgata cagctgggcg aaggcgccgc ggtccccttc tttggcgcgt
     6421 gcgaccagct gttcggcttc cggcgcggcc ggcgtcacct gaacggtgac cgacgacgcg
     6481 gaaacgctga cggacagatc gcccgactgc tgcgactccg cctgcttcac ggttagagca
     6541 tcgttttgac tcatcctcgg ccccagccgt ctctccctca agagacagcc cctccgttga
     6601 ggaatcgttc gccgggtagg cccggctcac cttcaacaac gacggagttt caaacctgtt
     6661 gcggggggcc cgaaaaattt ctgttcgtga ggatagaaaa cgattcgtcc tgcctatttg
     6721 ccctggtccg agagtatttt cacgacgccg agtcccaaaa atatttctcg cgataattgg
     6781 gcggcgacca ccggcgagcg accggaggga tcccagttcg ggctgacctc cacaaggtcg
     6841 acgccgacca cgttcaagcc ttgcagggac aaaagcgacg cgcgcagctc cttatacgag
     6901 catccacccg gctcgggcgt gccggtgccg ggaagctgac cggggtcgag cacgtcgagg
     6961 tcgcacgaga cgaagaccgg cctgccctgg agaagcgagc gcacctgcgg cacgatctcg
     7021 acctcggtgg accaataggc gagcatcttg tccgcgtcgg tgaactcctc gcgcgcgccc
     7081 gatcggatgc cgagcgcaat cgtggtgccg gccatggcca tatgccgtgt gccgcacgcg
     7141 tgcgacaggt cggtgccgtc gtaggaggcg cgcatgtcca tgtgcgcgtc gtagacgagg
     7201 tgcaccaggc cgggatgcac gcgccgcgcg ccgcgggtgg ttccgatcga gacgctgtgg
     7261 tcgccgccga ccgagatcag gaacttgccc gcgcgcaggt gccgctcggt ctcggccgcg
     7321 atcaggtcga tcgcgtcctc gggggccttg ccggcaaggg caaggtcgcc cgcgtcgaag
     7381 atcgcgtgat cctcgagatc ggcgcgcagc accggcgaat aggtctcgat cgagtcggac
     7441 gcgattcgca cggcggacgg cgcatcgcgc gcgcccgcgc gccacgacac gctgccgtcc
     7501 cagggcagcc cttggatgac cgcgaccgcc tcctcgtagg acgcgtccgc ggcgaggaac
     7561 ctggctccgt cggggatgcc
//
LOCUS       DATLFO010000300        12550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_77132, whole genome shotgun sequence.
ACCESSION   DATLFO010000300 DATLFO010000000
VERSION     DATLFO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77132"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VM841_12180"
     CDS             complement(<1..635)
                     /locus_tag="VM841_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005480913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL90981.1"
                     /translation="MRFVIIGGGPGGYEAALVASELGADVTVVDRAGLGGACVLWDCV
                     PSKTLCTTAEAVSWLEAAPELGLADEDLVGHRNVDLPKIFARITWLAEAQSRDIEKKM
                     AAAGVRVVRGSGRLDGTRRVAVTMEDGSAEMLDADTILIATGSSPREMPSAQPDGVRV
                     LNARQVYDLEGVPEHLIVVGSGATGAEFAHAFNRLGAQVTLVCSRDHVLPT"
     gene            complement(632..2200)
                     /locus_tag="VM841_12185"
     CDS             complement(632..2200)
                     /locus_tag="VM841_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVL90982.1"
                     /translation="MSEPTTPSGIPIRPLYEPDEDARAIAERLGTPGSFPFARGVYPD
                     MYRGKPWTMRQYAGYATARESNERYRYLLERGQTGLSVAFDLPTQMGYDSDDPVAAGE
                     VGKVGVAIDSIEDMRMLFDGIPLDQVSTSMTINAPASLLLLLYELVGEEQGVDPAKLT
                     GTIQNDILKEYIARGTYIFPPEPSMRLITDTFAYCAERLPRFNTISISGYHMGEKGAT
                     AVQEVAFTLADAIAYVEAGQRAGLDVDRFAPRLSFFFVARSSLFEEVAKFRAARLLWA
                     EIMRDRFGATNPKSQMLRFHTQTAGVQLTAQQPENNIVRVAIQALAAVLGGTQSLHAN
                     SFDEALALPTEKAAKIALRTQQILAHETGIDATPDPLAGSWYVEDLTRQVYERAKEYI
                     ATIDDRGGAVASIQFMQDEIEQAAYEEQKAVDAQQRIVVGVNRYAEDEAERVEILRVD
                     EGLTREQSERLASLRARRDDAAAKEALEDVRAAARGTGNLLPPMREALRRSCTIGEIC
                     GVLREIFGVYRPAR"
     gene            2286..3503
                     /locus_tag="VM841_12190"
     CDS             2286..3503
                     /locus_tag="VM841_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVL90983.1"
                     /translation="MADAEPRRTSTPPAGASGVGAEAVARILDIARSQLGMQVAFLAE
                     TGPTTPDRPAPASIRFTDGPAASFPLLAGGPALDALCAHVLNAAVPAIVPDVGAEAGY
                     PEGGDPSVGAFAAVPVTLSDGTPFGVIGALSHEPDPLLRDADAGMVRVLSRAVARLIE
                     QHGLTAPAARQAAIARIRTILEGTGLSMVFQPIVDLDSRMTLGYEALARFGAQPERSP
                     DRWFAEAARVDLGADLELAAIRAALSHMELLPAGAFLSVNASTPTVASPAFLAAVRGV
                     PAGKLVVELTDHIHIEDDRALGKALAALGMAGVQIATDVSDAGFETLSHLLQLTPDII
                     KLDGLLVRGIDTDPVRRVLGETIVAFAGEIGASVVGESIQTDAELAMLKAIGVRSGQG
                     RLLAPPGPLVGRG"
     gene            complement(3500..3991)
                     /locus_tag="VM841_12195"
     CDS             complement(3500..3991)
                     /locus_tag="VM841_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL90984.1"
                     /translation="MNGAVSVRPARSSDWAAVAALLAELGRPAALGAPEEPALREVFE
                     AYRTRADAAALVAESGGRIIGFCDVEFRVRLNFVEPQAWVPDLIVTEAARGTGAGRAL
                     LAEAERLARERGCWGMTLESAAWRTAAHAFYRHTGWTDAGLAFTKPLSDRVWPPPAPS
                     GRS"
     gene            4108..4488
                     /gene="sdhC"
                     /locus_tag="VM841_12200"
     CDS             4108..4488
                     /gene="sdhC"
                     /locus_tag="VM841_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HVL90985.1"
                     /translation="MRLPGKQTVYRGGSGQWSWAFHRISGIAVFLFLLAHIMDTALVG
                     FGPEAYNRVVAVYHHPIIRLLEIGLGAMVLFHALNGLRIIAIDFFPRLADKQRILFNY
                     VLGLYMILMAPLLYFMGKGFIESF"
     gene            4488..4922
                     /locus_tag="VM841_12205"
     CDS             4488..4922
                     /locus_tag="VM841_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HVL90986.1"
                     /translation="MATTTPTKPRPARRAAARPANGSKMELWTWYFMRLSGIVLVFLV
                     LGHFTIVHLIDTGVDRVDFAFVSGRWSSPFWQTYDAAMLVLGLMHGANGMRVVVEDYV
                     RPSRAGFYKGLLYTSAFAMLVLGLLIIITFDPNRGVSTVLKG"
     gene            4927..6663
                     /gene="sdhA"
                     /locus_tag="VM841_12210"
     CDS             4927..6663
                     /gene="sdhA"
                     /locus_tag="VM841_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HVL90987.1"
                     /translation="MTTIHEFDAVIIGAGGAGMRAALEASQHVRTAVITKLYPTRSHT
                     GAAQGGMCAALANVEEDNWEWHVYDTVKGGDFLVDQDAAEIMCKEAIDAVLELERWGL
                     PFNRTPEGKIDQRRFGGHTAEHGKAPVKRACYAADRTGHMILQTLYQQCIKQDVQFFN
                     EFYALDLVMSGDRVAAVIAYELATGELHVFRCKTAMFATGGYGKVFKVTSNAHALTGD
                     GCGVVLRRGLPLQDMEFYQFHPTGIYGIGILLSEAARGEGGILRNSTGERFMERYAPT
                     IKDLAPRDMVSRAIYFEIKEGRGVGPRKDHVLLHVDHLDPKVVEEKLPDITEFARIYL
                     GVEPLSEGVPIQPTAHYAMGGIPTNVDGQVVSDESGTPVPGLYAAGECACVSVHGANR
                     LGTNSLLDIVVFGRRAGKALAEEARRLTLADLPAAPERDVATMLEDLMDKARVDSPGR
                     IRAEMQQTMDDNASVYRTEETLKAQEAILEELQDRYARRVGVMDKSRSYNTDLLEAVE
                     LGFLLDLAQTLVHAARARTESRGGHYRGDFPTRDDVNWLKHSLTWKTGDGLKLGSRPV
                     TITNYQPEERKY"
     gene            6677..7348
                     /locus_tag="VM841_12215"
     CDS             6677..7348
                     /locus_tag="VM841_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HVL90988.1"
                     /translation="MTWTTSLDTIVEQRHLLDHPFYTAWTRGELTVDALRGYAGQYYK
                     HVAAFPTYLSAIHSRTEDIRTRQYLLENLNDEEQGEENHPELWLRFAEGLGATREDVR
                     AADALPETAECDRVFREIVTTASPAAALAALYAYESMVPAVAQSKIDGLKQHYGIEAG
                     EAIKFFTVHLEADVWHAQVARDLLEDAPEEVRAEAEDVTRRALDALWTLLDGVVREHA
                     PEAAA"
     gene            7345..8073
                     /locus_tag="VM841_12220"
     CDS             7345..8073
                     /locus_tag="VM841_12220"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HVL90989.1"
                     /translation="MSLLKKKAPPAEVEVQVLRFNPETDPEPHWESYMVPVEPMDRVL
                     DALHYIKWNIDGSLTFRRSCAHGICGSDGMLINGQNALACKALVKDYKSPILVEPIRG
                     LKTLKDLVVDMEPFFEAYRSVMPYLINDEPEPERERLQSAEDRERYDDTTKCILCACC
                     TTSCPIFWADEEYVGPAAIVGAHRFIFDSRDQGAGKRLTVLQDKEGAFKCRTTYNCTD
                     ACPRGIHVTRAIQEVKKEIMTRRI"
     gene            complement(8331..8519)
                     /locus_tag="VM841_12225"
     CDS             complement(8331..8519)
                     /locus_tag="VM841_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90990.1"
                     /translation="MLKKIFGLMVAGLLVFGGSAAFADGAPACEGPVSTDCDNDGDGV
                     EDCDLFVSIEGIELCENL"
     gene            complement(8687..9679)
                     /gene="trpS"
                     /locus_tag="VM841_12230"
     CDS             complement(8687..9679)
                     /gene="trpS"
                     /locus_tag="VM841_12230"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVL90991.1"
                     /translation="MARILSGIQPTGDVHLGNYIGAVRHWVTEQATDDCFYCIVDLHA
                     ITVPQDPAELRRKTLELSAILLAAGIDPERATLFVQSQVPQHAELAWVLNCFAMFGEL
                     RRMTQFKDKAGRQKEASVSVGLFDYPVLQAADILLYQADRVPVGEDQRQHLELARDIA
                     GRFNGRFGDTFTLPQAAIPKVGAKIFDLQNPAAKMSKSIDSPQGTVLILDPPEAIAKK
                     IKVAVTDSEREIVARPDKPAISNLLTIFAVATGNSIEDAESHFAGKGYGDLKRELTEA
                     LVEFLRPVREQTAELLGDSQQLAATLATGASKAEAVASKTLAGVYERVGFLPRG"
     gene            complement(9743..10960)
                     /locus_tag="VM841_12235"
     CDS             complement(9743..10960)
                     /locus_tag="VM841_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL90992.1"
                     /translation="MKRLSAAIALALALVACRGEPQPAGPVPDVLIVVNAALGTDAGR
                     QIERGAQLAVDQLNDTGGVRAGARTHRLTLTTMDTKGSALTAADNARTAIAQGAAAIV
                     GDGDGVEAVQGEAARLGVPVGIVMDAGGTIEPVSRPAVFRIAPAPADVAVRMREVLVA
                     ARLKTAVLHDDGPYGTAGLAALASAFDRHRESLAGSIPVSADDDGRDAVRRAEAAGAE
                     ALLLWMPAAMTARVIESAREAGWMRPIYTGPSGADPSVRVALAGRPEWMDGVVFASGR
                     LAGGPNPDDYGTFRAAYEKRFGAGEIPVTYDGQRVVDPPRWAMYSYDFVRLAAAGMTK
                     AGTVTPGARLLAGLESAAVEGANGATRRFTPRSHDALERDEIFFARFAEFFWAPVKDD
                     PRSANLPDVPQSG"
     gene            complement(10970..11902)
                     /gene="mdh"
                     /locus_tag="VM841_12240"
     CDS             complement(10970..11902)
                     /gene="mdh"
                     /locus_tag="VM841_12240"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HVL90993.1"
                     /translation="MKITVVGAGKYGSTTVQRLAEKGIADEIVMTDIVEGLPQGLALD
                     MMQSRSIEGFETRITGTNTYDETAGSDVCVVTAGLPRKPGMSRMDLLDVNAKIIRGVA
                     GQFCPNSPNAVVIVVTNPLDEMTFLFREASGFPAERVIGQAGMLDTARFKHFVAEELG
                     TSPNAVEAMTLGSHGETMVPVPSQVTVNGKPLTEVADAATVERLVQRTRDGGAEIVAL
                     LKTGSAYYAPSSAAAAMVEAVVRDSNAQMPVCAWLTGQYGIEGVYLGVPAKLGTGGVT
                     EIVDVGLADDELAALRDAAEAVRAKCADLANLAK"
     gene            12047..12454
                     /locus_tag="VM841_12245"
     CDS             12047..12454
                     /locus_tag="VM841_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL90994.1"
                     /translation="MPVYRMGEVAALLGVSPDTARRWADGGALKTRRTSGGQRVVDGK
                     DLAAFLRAEAHIDEAEGPPSSARNRFTGIVTAVVRDKAAASVEIHTGTARIVSLMTRE
                     AADDLGLEPGMLATASVKATNVTIGLPRGRAEP"
BASE COUNT         1996 a         4344 c         4242 g         1968 t
ORIGIN      
        1 tcggtgggca gcacgtggtc gcgtgaacag accagcgtca cctgcgcgcc caggcggttg
       61 aaggcgtggg cgaactcggc gccggtcgcg cccgagccga ccacgatcag atgctcggga
      121 acgccctcca ggtcgtagac ctgacgcgcg ttgagcacgc gcacgccgtc gggctgagcg
      181 gacggcatct cgcgcggcga cgagccggta gcgatcagga tcgtgtcggc gtcgagcatc
      241 tcggccgatc cgtcctccat cgtgaccgcg acccgccgcg ttccgtccag acggccgctg
      301 ccgcgcacga cccgcacgcc ggcggcggcc atcttctttt cgatgtcgcg ggactgggct
      361 tcggcgagcc aggtgatgcg ggcgaagatc ttcggcaagt cgacgttgcg atgcccgacc
      421 agatcctcgt cggccaatcc cagctcgggt gcggcctcca gccacgacac ggcctcggcg
      481 gtggtgcaca aggtcttcga cggaacgcag tcccacagca cgcaggcgcc gcccaagccg
      541 gcgcgatcga cgacggtgac gtcggcgccc agctcggatg cgaccaacgc ggcttcgtag
      601 ccgcccggcc cgccgccgat gatgacgaac ctcatcgcgc cggccggtag acgccgaaga
      661 tctcgcgcag gacgccgcag atctcgccga tcgtgcacga acggcgaagc gcctcacgca
      721 tcggcggcaa caggttgccg gtcccgcgcg ccgcggcccg cacgtcctcg agcgcctctt
      781 tcgccgccgc gtcgtcacgc cgggcgcgca gggatgccag ccgctcggac tgctcgcgcg
      841 tgagcccttc gtccacgcgc aggatctcga cgcgttcggc ttcgtcttcg gcgtatcggt
      901 tcacgccgac gacgatgcgc tgctgcgcgt ccacggcttt ctgctcttcg taggcggcct
      961 gctcgatctc atcctgcatg aactggatcg aagcgaccgc gccaccgcga tcgtcgatcg
     1021 tcgcgatgta ttccttcgcg cgttcgtaca cctggcgcgt cagatcctcg acgtaccacg
     1081 agccggccag cggatccggc gtcgcgtcga tgccggtctc gtgcgccaag atctgctggg
     1141 tgcgcagcgc gatcttcgcg gccttctcgg tcggcagcgc caaggcttcg tcgaacgagt
     1201 tcgcgtgcag cgactgggtg ccgcccagca ccgccgccag cgcctggatt gcgacgcgca
     1261 cgatgttgtt ctcgggctgc tgcgcggtca gctgcacgcc ggcggtctgg gtgtggaagc
     1321 gaagcatctg cgacttcggg ttggtcgcac cgaagcggtc gcgcatgatc tcggcccaca
     1381 gcagccgggc cgcccggaac ttggcgacct cttcgaacag cgacgagcgc gccacgaaga
     1441 agaacgacag ccgcggcgcg aagcgatcga cgtccaggcc ggcgcgctgg cccgcttcga
     1501 cgtaggcgat cgcatccgcc agcgtgaacg cgacttcttg cacggccgtc gcgcccttct
     1561 cgcccatgtg gtagccgctg atcgagatcg tgttgaagcg cggcagccgc tcggcgcagt
     1621 aggcgaacgt gtcggtgatc agccgcatcg acggctcggg cgggaagatg taggtcccgc
     1681 gcgcgatgta ctccttcaag atgtcgtttt ggatcgtgcc ggtcagcttc gccggatcca
     1741 cgccctgctc ttcgccgacc agctcgtaga gcaacagcag cagggatgcg ggcgcgttga
     1801 tcgtcatcga ggtcgagacc tgatccagcg ggatcccgtc gaacagcatc cgcatgtctt
     1861 cgatcgagtc gatcgccacg ccgaccttgc cgacctcgcc ggccgcgacc gggtcgtcgc
     1921 tgtcatagcc catctgcgtg ggcagatcga aggcgaccga cagaccggtc tggccgcgct
     1981 ccagcagata gcggtagcgc tcgttcgact cgcgcgcggt cgcgtagccg gcgtactggc
     2041 gcatcgtcca cggcttgccg cgatacatgt cgggatagac gccgcgcgca aacgggaacg
     2101 agccgggcgt gccgagccgc tccgcgatcg cgcgtgcgtc ttcgtccggc tcgtacagcg
     2161 gccggatcgg aatgcctgaa ggagtggtgg gttcgctcat cggccccatt ctagtggctc
     2221 gaattgccca ttgacccttc cggtcagaac ggcctagcgt gaacgcggga ccggagagga
     2281 atcgtatggc cgacgccgaa ccacgccgca cgtccacgcc gcccgccggc gcaagcgggg
     2341 tcggtgcgga agcggttgcg cgcatccttg acatcgctcg ctcgcagctg ggcatgcagg
     2401 tcgcattcct ggccgaaacc ggcccgacga cccccgaccg gccggcgccg gccagcatac
     2461 gcttcacgga cggaccggcc gcatcgtttc cgctgttggc cggcgggccc gccctggacg
     2521 cattgtgcgc gcacgtgctg aacgctgccg ttcccgcgat cgtgcccgac gtcggcgccg
     2581 aggccggcta tccggaaggc ggcgacccgt cggtcggcgc gttcgcggcc gtcccggtga
     2641 cattgtccga cggaacgccg ttcggcgtga tcggcgcgct gtcgcacgag cccgacccgc
     2701 tgctgcgcga cgccgacgcc ggcatggtcc gcgtgctatc ccgcgccgtc gcgcggctga
     2761 tcgaacagca cggcctgacc gcccctgccg cgcggcaagc tgcgattgcg cgcatccgga
     2821 ccatcctgga gggaaccggc ctgtcgatgg tgttccagcc gatcgtcgat ctggattcgc
     2881 ggatgacgct gggctacgag gcgctggccc gattcggcgc gcagccggaa cgctcgcccg
     2941 atcgctggtt cgccgaggcc gcgcgcgtgg atctgggcgc cgatctggag ctggccgcga
     3001 tacgcgccgc gctgtcgcac atggagctgt tgcccgccgg cgcgttcctg tcggtcaatg
     3061 cctcgacgcc gacggtcgcg tcgccggcgt tcctggccgc cgtccgcggc gtgcccgccg
     3121 gcaagctggt cgtcgagctg accgatcaca tccacatcga ggacgaccgg gccctgggca
     3181 aggcgctggc ggcgctcggc atggcgggcg tgcagatcgc caccgacgtc tcggacgcgg
     3241 gcttcgagac cttgtcgcat ctgctgcagc tcacgcccga catcatcaag ctggacggcc
     3301 tgctggttcg cggcatcgac accgacccgg tgcgccgagt gctgggcgag acgatcgttg
     3361 cgttcgccgg cgagatcggg gcatcggtcg tgggcgagtc gatccagacc gacgccgagc
     3421 tggcgatgct caaggcgatc ggcgtgcgat ccggtcaggg ccggctgctc gccccgcccg
     3481 gaccgctggt cggtcgcggc taagaccggc ccgaaggcgc aggcggaggc cagacgcgat
     3541 ccgataacgg cttggtgaac gccagcccgg cgtcggtcca tccggtgtgg cggtagaagg
     3601 cgtgcgccgc ggtccgccac gcggccgact ccagcgtcat gccccagcag ccacgctcgc
     3661 gcgccagccg ctcggcctcg gccagcagcg cgcgtcccgc gccggtgccg cgcgccgcct
     3721 cggtcacgat gagatccggc acccacgctt gcggctcgac gaagttcaac cggacgcgga
     3781 actccacgtc gcagaagccg atgatcctgc cacctgattc ggcgacgagc gctgccgcat
     3841 ccgcccgcgt ccggtacgcc tcgaagacct cgcgcaacgc gggctcctcc ggcgcgccga
     3901 gcgcggcggg acgccccagc tcggcaagca gcgcggcaac cgcagcccag tccgaggacc
     3961 gggcagggcg gaccgaaacg gcaccgttca cgacgcgaat cctacgtccg ccctttccgg
     4021 tttgggagcc gatttaaggg gtgctaggat tcgccgcgcg gcgccttccg gcgccgaaaa
     4081 cgacctccga gggggaccgg aaccgacgtg cgactgcccg gaaaacagac cgtctatcgg
     4141 ggcggctcgg ggcaatggtc ctgggcgttt caccggatct ccggcatcgc ggtcttcctg
     4201 ttcctgctgg cccacatcat ggacacggcg ctggtcggct tcggccccga ggcctacaac
     4261 cgggtcgtgg cggtctacca ccatccgatc atccgcctgc tcgagatcgg cctgggtgcg
     4321 atggtgctgt tccacgcgct caacggcctg cgcatcatcg cgatcgactt cttcccgcgc
     4381 ctggccgaca agcagcgaat cctgttcaac tacgtcctcg gcctctacat gatcctgatg
     4441 gcccccctgc tgtacttcat gggcaaggga ttcatcgagt ccttctgatg gcgactacga
     4501 ccccgaccaa gccgcggccg gcgcgccgcg ccgccgcgcg tcccgcgaac ggctccaaga
     4561 tggagctatg gacctggtac ttcatgcgtc tgagcggcat cgtgctggtg ttcctggttc
     4621 tcggccactt cacgatcgtg cacctgatcg acacgggcgt ggaccgcgtg gacttcgcct
     4681 tcgtctcggg ccgctggtcc agcccgttct ggcagaccta cgatgcggcg atgctggttt
     4741 tgggcctgat gcacggcgcc aacgggatgc gcgtcgtcgt cgaggactac gtgcggccga
     4801 gccgcgcggg cttctacaag ggtcttctgt acacctcggc attcgcgatg ctggtgctcg
     4861 gccttctgat catcatcacc ttcgacccga accgcggggt ctccaccgtt ctcaagggat
     4921 gagccgatga ccacgatcca cgagttcgac gcagtcatca tcggcgcggg cggcgccggg
     4981 atgcgcgcgg ccctggaagc ctctcagcac gtgcgcaccg ccgtgatcac caagctgtat
     5041 ccgacgcgct cgcacacggg tgcggcgcag ggcggcatgt gcgctgcgct cgccaacgtc
     5101 gaagaggaca actgggagtg gcacgtctac gacacggtca agggcggcga cttcctggtg
     5161 gaccaggacg cggccgagat catgtgcaaa gaggcgatcg acgcggtctt ggagctggaa
     5221 cgctggggct tgccgttcaa ccgtacgccc gaaggcaaga tcgatcagcg gcgcttcggc
     5281 ggccacaccg ccgagcacgg caaggcgccg gtcaagcgcg cctgctatgc cgcggaccgc
     5341 accggccaca tgatcctgca gaccctgtac cagcagtgca tcaagcagga cgtgcagttt
     5401 ttcaacgagt tctacgcgct ggacctggtc atgtccggcg accgggtcgc ggccgtgatc
     5461 gcctacgagc tggcgaccgg cgagctgcac gtgttccgct gcaagaccgc gatgttcgcc
     5521 accggcggct acggcaaggt gttcaaggtc acctcgaacg cgcacgcgct gaccggcgac
     5581 ggctgcggcg tcgtgctgcg gcgcggcctt ccgctgcagg acatggagtt ctaccagttc
     5641 catcccacgg gcatctacgg gatcgggatc cttctgtccg aggccgcgcg cggcgaaggc
     5701 gggatcctgc gcaactcgac cggcgagcgg ttcatggagc gctacgcgcc caccatcaag
     5761 gatctggcgc cgcgcgacat ggtcagccgc gcgatctact tcgagatcaa agaaggccgc
     5821 ggcgtcgggc ccaggaaaga ccacgtgctg ctgcacgtgg atcacctcga tccgaaagtc
     5881 gtcgaggaga agcttcccga catcaccgag ttcgcgcgca tctacctggg cgtcgagccg
     5941 ctcagcgagg gcgtgccgat ccagccgacc gcgcactacg cgatgggcgg catcccgacc
     6001 aacgtggacg gccaggtcgt cagcgacgaa tcgggaacgc cggtgccggg tctgtacgcc
     6061 gccggcgagt gcgcgtgcgt gagcgtgcac ggcgcgaacc ggctgggcac gaactcgctg
     6121 ctggacatcg tggtgttcgg ccggcgcgcc ggcaaggcgc tggccgaaga ggcgcgccgc
     6181 ctgacgcttg cggacctgcc cgccgcgccc gagcgcgacg tcgccacgat gctggaggac
     6241 ctgatggaca aggcgcgtgt ggactcgccc ggccggatcc gcgccgagat gcagcagacg
     6301 atggacgaca acgccagcgt ctaccgcacc gaagagacgc tcaaggcgca ggaggcgatc
     6361 ctggaagagc tgcaggaccg ctacgcgcgc cgcgtcggcg tgatggataa gtcacgcagc
     6421 tacaacaccg acctgctgga ggcggtcgag ctgggcttcc tgctcgacct tgcgcagacg
     6481 ctggtgcatg cggcccgcgc gcgcaccgaa agccgcggcg gccactatcg cggcgacttc
     6541 ccgacccgag acgacgtgaa ctggcttaag cactcgctga cctggaagac gggcgacggc
     6601 ctgaagctgg gctcgcgtcc ggtgacgatc acgaactatc agcccgaaga gcgaaagtac
     6661 taagggggcg gccaccatga cctggacgac gagcctggac accatcgtcg agcagcggca
     6721 cctgctcgac catcccttct acacggcctg gacccgcggc gagctgaccg tcgacgcgtt
     6781 gcgcggctac gccggccagt actacaagca cgtcgcggcc ttcccgacgt acctgagcgc
     6841 gatccactcc cgcaccgagg acatccgcac gcgccagtac ctgctcgaaa acctcaacga
     6901 tgaagagcag ggcgaggaga accaccccga attgtggctg cgcttcgccg aaggcctcgg
     6961 cgcgacgcgc gaggatgttc gcgccgcgga cgccctgccc gagacggccg agtgcgaccg
     7021 cgtgtttcgc gagatcgtca cgacggcttc gccggccgcg gcgctggcgg cgctctacgc
     7081 ctacgagtcg atggtcccgg cggtcgcgca gtccaagatc gacggcctga agcagcacta
     7141 cggcatcgag gccggcgagg cgatcaagtt cttcacggtg catctggagg ccgacgtgtg
     7201 gcacgcgcag gtcgcccgcg atctgctcga ggacgcgccc gaggaggttc gcgccgaggc
     7261 cgaagacgtg acgcgccgcg cgctggacgc actgtggacg ctgctggacg gcgtggtccg
     7321 agagcacgcg ccggaggccg ctgcgtgagc cttttgaaga agaaggctcc accggccgag
     7381 gtcgaggttc aggtccttcg gttcaacccc gagaccgatc ccgagccgca ctgggagtcc
     7441 tacatggtcc cggtcgagcc gatggatcgg gtgctggacg cgcttcacta catcaagtgg
     7501 aacatcgacg gctcgctgac gtttcgccgc tcgtgcgcgc acggcatctg cggatccgac
     7561 ggcatgctga tcaacgggca gaacgcgctg gcgtgcaagg cgctggtcaa ggactacaag
     7621 agcccgattc tggtcgagcc gatccgcggc ctcaagacgc tcaaggatct ggtcgtggac
     7681 atggagccgt tcttcgaggc gtaccgcagc gtgatgccgt acctgatcaa cgacgagccc
     7741 gagcccgagc gcgaacggct gcagtcggcc gaggatcgcg agcgctacga cgatacgacc
     7801 aagtgcatcc tgtgcgcgtg ctgcacgacc tcgtgcccga tcttctgggc cgacgaggag
     7861 tacgtcgggc cggccgcgat cgtgggtgcg caccggttca tcttcgactc gcgcgaccag
     7921 ggcgccggaa agcggctgac cgtgctgcag gacaaggaag gcgcgttcaa gtgccgcacc
     7981 acctacaact gcaccgacgc ctgcccgcgc ggcatccacg tgacccgcgc gatccaagag
     8041 gtcaaaaaag agatcatgac ccgcaggatc tagggcagcg cgtcaaaccg aatacgcagc
     8101 gtaggtccgg gaaatgggag aggggacgac ccccatggtc atcccctcga agttcgacga
     8161 cttccggttt cgtttccgct ccgccgacgc gccgtcgttg cgcgtgtttt cgctggtcgc
     8221 cggcgctctt cctgtcgcgc tacggaaact gccggatcgt tcccccggcc aaaggtcgtg
     8281 aaaacgaacg aggagggttc aaatgaaccc tcctcgtttc aaagcacgac ttagaggttc
     8341 tcgcaaagct cgataccctc gatcgacacg aacaggtcgc agtcctcgac tccgtcgccg
     8401 tcgttgtcgc agtccgtcga caccgggccc tcgcacgccg gggccccgtc agcaaaagcc
     8461 gccgagccgc cgaacacgag aagaccggcc accatgagac cgaaaatctt cttcagcata
     8521 aagttccccc cccttctgtt cccgcgcatc cccacgcagg ttggtgatcc tagagccccc
     8581 tgatgctcct tttcggggcc tctgcgtaag attacggggc cgggtatgcc ctcttacagg
     8641 gggtgcgtgc ggaagtatcc atttgaacag aaattggggt gcccactcac ccgcgaggca
     8701 agaaaccgac acgttcgtag acgccggcga gcgtcttgga ggccacggcc tcggccttgc
     8761 tcgcgccggt ggccagggtc gctgcgagct gttgcgagtc ccccaggagc tccgccgttt
     8821 gctcgcggac gggtcgcagg aactcgacga gcgcttcggt cagttcgcgc ttgagatccc
     8881 cgtagccttt gccggcgaag tgcgactccg cgtcctcgat cgagttcccg gtcgcgaccg
     8941 cgaagatcgt caggaggttc gagatcgccg gcttgtcggg ccgggcgacg atctcacgct
     9001 cggaatcggt cacggcgacc ttgatcttct tggcgatcgc ctcgggggga tcgaggatca
     9061 gcacggtccc ttgcggcgag tcgatcgact tcgacatctt ggctgccggg ttctgcagat
     9121 cgaagatctt cgcgccgacc ttcgggatcg cggcctgcgg cagcgtgaac gtgtccccga
     9181 agcggccgtt gaaacgtccc gcgatgtcgc gcgccagctc gagatgctgg cgctggtcct
     9241 cgcccaccgg gacgcgatcg gcttgataca gcaggatgtc ggccgcctgc aggaccggat
     9301 agtcgaacaa tccgacggag acgctggctt ccttctggcg gccggccttg tccttgaact
     9361 gggtcatccg ccgaagctcg ccgaacatcg cgaagcagtt caggacccag gcgagctcgg
     9421 cgtgctgcgg gacctgcgac tggacgaaca gcgtggcccg ctcggggtcg atgccggcgg
     9481 ccagcagaat cgccgacagc tcgagcgtct tgcggcgaag ctccgccgga tcctgcggca
     9541 cggtgatcgc gtgtaaatcg acgatgcagt agaagcagtc gtcggtcgcc tgctcggtca
     9601 cccagtgacg gacggcgccg atgtagttgc ccaggtgcac gtcgccggtg ggctggatgc
     9661 ccgacagaat cctcgccacg atctcgcgtc cctcctgtgc cgtgcggttc gccgcacgat
     9721 aacaagacgg cgaatggtgc gcctatccgc tctgcggcac gtccgggagg ttggccgagc
     9781 gcggatcgtc cttcaccgga gcccagaaga actcggcgaa ccgggcgaag aagatctcgt
     9841 cccgctccaa ggcatcgtgc gagcggggcg tgaagcgccg cgtcgcgccg ttggcgcctt
     9901 cgacggcggc agactccaag ccggccagca acctcgcgcc cggcgtgacg gtgccggcct
     9961 tggtcatgcc cgccgccgcg aggcgcacga agtcgtagga gtacatcgcc catcgcggcg
    10021 gatccaccac gcgctgcccg tcgtacgtca ccgggatctc gccggctccg aagcgcttct
    10081 cgtaggccgc gcggaaggtc ccgtagtcat cgggattcgg cccgccggcc agccgtccgg
    10141 acgcgaagac gaccccgtcc atccattccg ggcgcccggc gagggcgaca cggaccgacg
    10201 ggtctgcgcc cgagggaccg gtgtagatcg gccgcatcca gccggcttcg cgcgcgcttt
    10261 cgatgacgcg ggcggtcatg gccgctggca tccacagcaa cagcgcctcc gcccccgcgg
    10321 cctcggcccg ccggactgcg tcgcgaccgt catcgtccgc ggagaccggg atcgatcccg
    10381 ccaagctctc gcgatgacga tcgaaagcgg atgcgagcgc ggcgagcccg gcggtgccgt
    10441 aggggccgtc gtcgtgaaga accgcggtct tgagtcgcgc ggcgaccagg acctcgcgca
    10501 tgcgcacggc aacatccgcc ggcgcgggcg cgatacggaa gacggcgggc cgcgaaacgg
    10561 gttcgatcgt gcctccggcg tccatcacga tgccgacggg cacgcccaaa cgcgcggctt
    10621 cgccctggac ggcctcgacg ccgtcgccgt ccccgacgat cgccgcggct ccctgggcga
    10681 tcgccgtgcg agcgttatcc gcggcggtca gcgccgagcc cttggtatcc attgtcgtga
    10741 gcgtgagccg gtgcgtccgc gcgccggctc gcacgccccc ggtatcgttt aactgatcca
    10801 cggccagctg cgcgccgcgc tcgatctgcc gtcccgcgtc ggtgccgagc gcagcgttga
    10861 ccacgatcag cacgtcggga accgggccgg cgggctgggg ctcgccgcgg cacgcgacga
    10921 gggcgagcgc cagcgcgatc gcagcggaaa ggcgcttcaa cagggccgct tacttggcga
    10981 ggttcgcgag gtccgcgcac ttggcgcgca cggcctcggc ggcgtcgcgg agcgcggcga
    11041 gctcgtcgtc ggccaggccg acgtcgacga tctcggtcac gccgcccgtg ccgagcttgg
    11101 cgggcacgcc caggtacacg ccctcgatcc cgtactgccc ggtcagccac gcgcacaccg
    11161 gcatctgcgc gttcgagtcg cgcacgaccg cctcgaccat cgcggccgcg gcgctcgagg
    11221 gcgcgtagta cgcggagccg gtcttgagca gcgccacgat ctcggcgcca ccgtcgcggg
    11281 tgcgctgcac gagccgctcg acggtggccg cgtcggcgac ctcggtcagc ggctttccgt
    11341 tgaccgtcac ctgcgacggg accggaacca tcgtctcgcc gtgtgagccg agcgtcatcg
    11401 cctcgaccgc gttcggggac gtaccgagct cttcggcgac gaagtgcttg aagcgcgcgg
    11461 tgtcgagcat gccggcctga ccgatcaccc gctcggccgg gaaacccgaa gcctcgcgga
    11521 acaagaaggt catctcgtcg agcgggttgg tgaccacgat cacgaccgcg ttcggcgagt
    11581 tggggcagaa ctgaccggcg acgccgcgga tgatcttggc gttgacgtcg agcaagtcca
    11641 tgcggctcat gccgggcttg cgcggcaagc cggcggtgac cacgcacacg tccgagccgg
    11701 cggtctcgtc gtaggtgttg gtgccggtga tgcgagtctc gaacccttcg atcgatcgcg
    11761 attgcatcat gtccagcgcc aagccctgcg gcaggccctc gacgatgtcg gtcatgacga
    11821 tctcgtcggc gatgcccttc tcggcgagcc gctggaccgt cgtggatccg tacttgcccg
    11881 cccctacgac cgtgatcttc atggttgtgc tctccctcga cgcgtgtgtg gctccaatcc
    11941 tgttcgcaaa ccgcgacgga cgcaaaacgc ggccgcaggc gctcgggcac gagatcgaca
    12001 ggatatttct tgcacatacc aatttctatt cggtaatctc cggcttatgc cggtttaccg
    12061 catgggagag gttgccgcgc tgctgggcgt gagccccgac accgcgcggc gttgggcgga
    12121 tggcggggcg ctcaagaccc ggcgcacgag cggcggccag cgcgtggtgg acggcaagga
    12181 cctggccgcg ttcctgcgcg ccgaggcgca catagacgag gccgaaggac cgccgtcgtc
    12241 ggcccgcaac cgcttcaccg ggatcgtgac cgccgtcgtg cgggacaagg cggccgcctc
    12301 ggtcgagatc cacaccggca cggcccggat cgtgtcgctg atgacgcggg aggcggccga
    12361 cgatctcggc ctcgagcccg gcatgctcgc gaccgcctcg gtcaaggcga ccaacgtcac
    12421 gatcggcctg ccgcgcggcc gcgccgagcc atgaagcgcc tcgctgcgct cgcgctgacc
    12481 ctgggcgcgg tgctgagcgg atgcgcgggc ggagccgacg cgacgctgac ggtcttcgcg
    12541 gcctcgtcgc
//
LOCUS       DATLFO010000301         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_77139, whole genome shotgun sequence.
ACCESSION   DATLFO010000301 DATLFO010000000
VERSION     DATLFO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77139"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VM841_12250"
     CDS             complement(<1..719)
                     /locus_tag="VM841_12250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL90995.1"
                     /translation="MINAPMPWLEPVRGGYPDPRVFGLPGLGQSEMFLKNGGPRPPMS
                     HLTGLMFREAGDGRAVFTLPATDWFLSSQDQISAGAITMLADAAVAAAIFHTLPSATP
                     MTTWDLTMTFLRPCPAGGELRATGKLLRSDRPLSLAEVWVEDDQGQQVAHGTSSCFIL
                     PSIDGVPEPEDLSIFGYDAPVYDTPDPYLRPAPGEVIPWDRWRALSGIEILQGQIAGD
                     LPAPPIHYLTGMTLTEAAEGSA"
     gene            914..>2016
                     /locus_tag="VM841_12255"
     CDS             914..>2016
                     /locus_tag="VM841_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90996.1"
                     /translation="MRRITLVLAMVASAITVIAPRPAPAAGPDYEWALPEWRAAFAAP
                     RLADNRGFVVQTTSDAVYVAGVAGAEGFGAGDAMVLRYGLDGRLVWSRAWGGLDLDQA
                     HDMAVDGDDIWIAGSYQDASGRTLGAVLNLTTDGGLQSARTYDWGVATLFQSVATTGS
                     GVVVGGFTHPTRDDRDAVIASIEAGGAVGWTAHIPGDGWSEFWDVAVAADGADVVAAG
                     YHGPATGTSQALIRRFGIDGTPLQSVSFGGDSNDEARAIQVTGDAIYVTGGSQAGRST
                     DVFVARFGRDLAQQWLRTTGGEVVGGGGYGIALGERGIYVAGGTYDFPAGGDSALMRF
                     GFDGTLEWTQVAGLPGFWDWNFDVAVRGGRLYTT"
BASE COUNT          301 a          685 c          722 g          308 t
ORIGIN      
        1 gcgctgcctt cggccgcctc ggtcagcgtc atccccgtca gatagtggat cggcggagcg
       61 ggcagatccc cggcgatctg accctgaagg atctcgatgc cgctgagcgc tcgccatcga
      121 tcccacggaa tcacctcgcc cggcgcgggc cgcaggtacg gatcgggcgt gtcgtagacc
      181 ggcgcgtcgt acccgaagat cgacaggtcc tcgggttccg gcacgccgtc gatcgagggc
      241 aggatgaagc agctggacgt tccgtgcgcg acctgctgtc cctgatcgtc ctcgacccag
      301 acttcggcca gcgacagggg ccggtccgag cgaagcagct ttccggtcgc acgaagctcg
      361 ccgccggccg ggcaaggacg caggaacgtc atcgtcagat cccacgtcgt catcggtgtc
      421 gcagacggca gcgtgtggaa gatcgccgcc gccaccgcag cgtccgccag catggtgatc
      481 gctccggccg agatctgatc ctgcgaggac aagaaccagt ccgttgccgg aagcgtgaac
      541 acggccctgc cgtcaccggc ttcccggaac atcagaccgg tcaggtgtga catcggcggc
      601 cggggcccgc cgttcttcag aaacatctcg ctctgcccga gccccggcag accgaagacg
      661 cgggggtcgg gatagccgcc gcgaacgggc tcgagccacg gcatcggtgc gttgatcatg
      721 gccgacagac tcgaccgtcc cggccgggaa cagcaaatcg gcacggcatt cctgcgggct
      781 ggatcctgtc gctcccgatc gtgggccgcc ggggccggtt gggcgacgga gcagttcccc
      841 gagagaggga ttgccggttc gtacgcagaa gtacgagcaa tcggacgttg cagccatcac
      901 cgaaggagga ctcgtgcgcc gcatcacgct cgtgcttgcc atggtcgcct ccgcgatcac
      961 cgtcatcgct ccgcggcctg cccctgcggc cggaccggat tacgagtggg cgcttcccga
     1021 gtggcgagct gccttcgcgg cgccacgcct ggcggataac cgcggcttcg tcgtgcagac
     1081 gaccagcgac gcggtctatg tcgcgggggt cgccggggcc gaaggattcg gcgccggcga
     1141 cgccatggtg ttgcgctacg gcctggacgg ccgcctcgtg tggtcgcggg cgtggggcgg
     1201 tctcgatctg gatcaggcgc acgacatggc ggtcgacggc gacgacatct ggatcgccgg
     1261 gtcgtatcag gacgcgtcgg ggcggacgct cggcgcggtg ctgaacttga cgaccgacgg
     1321 cggtcttcag tcggcgcgca cctacgactg gggcgtggcg acgctgttcc aaagcgtcgc
     1381 caccacggga tcgggtgtcg tcgtcggcgg cttcacgcat cccacgcgcg atgatcgcga
     1441 cgcggtgatc gcgtcgatcg aggccggcgg agccgtcggc tggaccgcgc acatcccggg
     1501 cgacggctgg tccgagttct gggacgtcgc cgttgccgcc gacggcgccg acgtggtcgc
     1561 ggcgggatac cacggccccg cgaccggcac ctcgcaggcg ctgattcgcc gcttcggcat
     1621 cgacgggacg ccgctgcaga gcgtttcctt cggcggcgac agcaacgacg aagctcgcgc
     1681 gatccaggtc accggcgacg cgatctatgt caccggcggc tcgcaggccg gtcgcagcac
     1741 cgatgtgttc gtcgcgcgct tcggacgcga cctcgcgcag caatggctcc gaacgaccgg
     1801 cggcgaggtc gtcggcggcg gcgggtacgg gatcgcgctc ggcgagcgcg gcatctacgt
     1861 cgccggcggc acctacgact tcccggccgg cggggattcg gccctgatgc gcttcggctt
     1921 cgacggcacg ctggagtgga cgcaggtcgc cggcctgccg ggcttctggg actggaactt
     1981 cgacgtcgcg gtacgaggcg gccggctcta cacgac
//
LOCUS       DATLFO010000302         7387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_97142, whole genome shotgun sequence.
ACCESSION   DATLFO010000302 DATLFO010000000
VERSION     DATLFO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97142"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1197)
                     /locus_tag="VM841_12260"
     CDS             complement(76..1197)
                     /locus_tag="VM841_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL90997.1"
                     /translation="MSASRRTILLVATAAMLLAVFAVLPAPELIFRRSPIVSDRIAGD
                     VRRIAMTVEPDGAMHAALEVWTDSDWDIEYWSSITNLTPVARIVTLDLIEQRDASIAM
                     AGDRLALAWIDVDAKAKRYALQVATKRRIDRSWAKPVVVAQGNRPLREAAVAIDAAGR
                     IVVAYVDGPDLRIASVDSGSATPPARLAAGARRPSIALEPGTGDAVVAYEVRDGSVSS
                     VRAGPLEAPRPKGAASPLARTGRAPFVAAAGATVAVAWAERDAVVVRRGGEAQQRVAA
                     PGLGDQRPSIAFTRGGPVAAWAARGRVVLLGADGIVRDLTPVRGCCPSVSATGSGMWV
                     AWLESRPGNAIHRLARLRYEGEPGRFTTGDRIFVTELPG"
     gene            1222..2691
                     /locus_tag="VM841_12265"
     CDS             1222..2691
                     /locus_tag="VM841_12265"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVL90998.1"
                     /translation="MATRPARLASPEPLVGAGLALAAIAGAMLLRQWTLLLVPFIWLA
                     IAALSRAALPLRFAWLAAATVLLPRYPLGFGLSVDDLLPSAAAIAGVVLLARWGFPRL
                     PRWMESAFAIWLVAAAASAVVNGQSLGEIARLAGPGVGRPVFWFVFTTTAAAIAGRLG
                     VRAILIPIAAVAIIEAAFSIAAYTVCGEIHLGAVSRPENESVWDARRALGVEQGAGTN
                     VGEQRIRCRATGTLGQSSNFLAAFLVTTLPVAAALTLAKRQRAGRLRWGAGTVALAAA
                     LVLTFTRASLIAVAIALLVTLLLAAPRRALPVVVIAGLVLGTASLAIPQVKRRLTDSA
                     NDRRALWWSGVAIFKDHPLMGVGFGNYREVQLSDRKYLDTPYGEPTSTAHNGFIAIAA
                     EGGVVQAGAVLVLAGGILVLGWRASRSARGTPDAALVAGALGGCAGFLVQNMTNTLLL
                     VPTVATYFWVMGGVLAGVAATGAPPGDERGREHAEITAE"
     gene            complement(2614..4104)
                     /locus_tag="VM841_12270"
     CDS             complement(2614..4104)
                     /locus_tag="VM841_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HVL90999.1"
                     /translation="MTGTTGKPMTETTPSHVRLARNTALSLVAIGLLGAGRLVYNIVV
                     GRRFGVAALGEATLAINAAMFATLLVSGGWQTSTAKYVAQAAGGGDRALAAGIYRRTL
                     LHNASGGVVVAALVAAVLAVVPEAGLRTRDAILAGAIAFAYNAYQHLKSAHYGFGMVE
                     SYLRLEALSSGAMLAALAVVAVAATRSAILWPVAGGYALFAILSAARLRGGGAAKPVP
                     APLWREIRGFTALAIAGTVASAGFLNLSPVFVGRYDSPRMLGVFSAALALVIPVYFVP
                     RALSLALFPSIAYRYGEERLDSVRRQLHETGRALWILLLAPVAAAAVTAPVILRWLFG
                     AGFDDGALILTIMMGATYLSVVQIPYVTTLSGTDRRYYKVPVLASIAGFGVGVGVWVT
                     LGARDGATGVAWGYLIGSVPQAAIPIAFAVRTFGVRIASVVVRAAMLWVIVAAIVWQV
                     RIDETGSAMIAVLGVLAVYAVLFGRDLGVLAASLVARRRAGRGDAG"
     gene            4170..5123
                     /locus_tag="VM841_12275"
     CDS             4170..5123
                     /locus_tag="VM841_12275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91000.1"
                     /translation="MERLLVVSNMYPSPKAPYFGVFVQRQVRALQAHGVDVALAVSDR
                     RGGSVAGAARKYAALGLDAHRVAKRSHPWAVLAHFVFPSGVIALSAGRTARAPVALVA
                     HGGDVDPNKPAWVARATREVLARVDLLLAVSRSIAGEAQAAGADPERIAIASMGYDAD
                     AFVPRPRPEARTRLGIPEGRQLAVMVGNLIPRKGVGVIVDAAAALAPAHPDLQWVLVG
                     GGDQARWRAQAERAGSAAAIRFTGPADPEDVPWWLSAADVAVVPSLREPYGVAAVEAL
                     ACGVPVVASRTGGLAEIMDGDHGLTVEPGDAASPSGAPPRR"
     gene            complement(5120..6481)
                     /locus_tag="VM841_12280"
     CDS             complement(5120..6481)
                     /locus_tag="VM841_12280"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HVL91001.1"
                     /translation="MLYLAGIALGLLAGFAMRGRLRWLAASVRFPWVWLLYVAVLAQV
                     GAGFVSASWLTRDVKFWIVIGSYGLLIVWLGVVAAAHRAWLRVAFLIVGAGTALNLVA
                     IAPNRGIPVSRAALRAANAPIPSGEENRFALKHVFASDTTVLNWLGDVIPVRPIRDVI
                     SIGDIVIGLGIAWVIAAGMRARPREDSPAGDEASGQGAGVMPPAPGMADILNLRSRRL
                     APGMAELLGLRSGRLAPDTTVADFLGRFARDYAVGHAYDPMSEQSRHPSESRVSLSQA
                     MSITDANLAGNVHGGVIMKLVDTAAGLSAIKHAHGRVVTVSMDEMSFIEPVFLTDVVT
                     LTAQVNDVGRTSMEVGVRVEAENTVTGERRHVSSAYLVFVALDEEGKPRPVPRLDPET
                     DEERRRQDEAKIRRRHRIASKEAVLARRQAQERGETPPPPPDPASAPGGIVPPGDIVP
                     PGA"
     gene            6552..>7387
                     /locus_tag="VM841_12285"
     CDS             6552..>7387
                     /locus_tag="VM841_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91002.1"
                     /translation="MRSRFRPAVVAAMLAAVVGGIAAPAPASSIDAGGRLVVAVELKA
                     MRGDAVTVCQDQPFGEGVETFADTVAAAFGLPLTGVNASPSSDCFALNLTGPLTTRGP
                     GGARILRFDAGALSSIASEAGFGEAFLVVCTPNVTDRIQVLTGDRPARASGCDAKGYA
                     WSLADPLEIEVAFRATGADLAEGLLAAAAWWIAFATLLGIAVVRRAQLGLLRRMPLLG
                     GAAGALVAGAAAYGWIFVASRSPLVDALAMLAGLGSAGEVALLASGSALALALTAVGA
                     RR"
BASE COUNT         1035 a         2725 c         2584 g         1043 t
ORIGIN      
        1 gcgcgcggat gacaggcggc cgcggaggtt gaacgttcgc ggtgcggtcc ggtgggaggc
       61 ggcaggtggg gtgggttagc cgggaagctc ggtgacgaag atgcggtcgc cggtggtgaa
      121 gcggccgggc tccccctcgt atcggaggcg cgcgaggcga tggatggcgt tgccgggccg
      181 ggactcgagc caggccaccc acattccgga gcccgtggcc gagaccgacg gacagcagcc
      241 gcgaacgggc gtgaggtcgc ggacaatacc gtcggcgccg agcaggacaa ctcgtccgcg
      301 tgcggcccag gcggcgaccg gcccgcctct ggtgaacgcg atggacggac gctgatcgcc
      361 cagacccggc gctgcgactc gctgctgcgc ctcgccccca cggcgcacca cgacggcatc
      421 ccgctcggcc cacgcgaccg cgaccgtcgc tcccgccgcc gcgacgaacg gcgcgcggcc
      481 cgttcgcgcc agcggcgatg ccgccccttt cggccgcggc gcctcgagtg gcccggcccg
      541 gacggacgag acactgccgt cgcggacctc gtaggcgacg acggcgtctc cggtccccgg
      601 ctcgagcgcg atcgaaggcc gccgcgcccc ggccgccagc cgcgccggcg gcgtcgcgct
      661 gcccgaatcc acggacgcga tccgaaggtc cggaccatcg acgtatgcga cgacgatccg
      721 cccggccgca tcgatcgcga cggcggcctc tcgaagcgga cgattcccct gcgccaccac
      781 gaccggcttc gcccagctgc ggtcgatccg gcgcttggtg gccacctgca gcgcgtagcg
      841 cttcgccttc gcatcaacat cgatccatgc gagcgcgagc cggtcaccgg ccatcgcgat
      901 cgaggcgtcg cgctgctcga tcaggtccag cgtgacgatg cgggcgaccg gcgtcaggtt
      961 cgtgatgctc gaccagtact cgatgtccca gtccgagtcg gtccacacct cgagcgccgc
     1021 gtgcatcgca ccatcgggct cgaccgtcat cgcgatgcgg cggacgtctc ccgcgatgcg
     1081 atcggacacg atcggcgaac ggcgaaaaat cagctcggga gccggcagga ccgcgaagac
     1141 ggcgagcaac atggcggccg tcgcgaccag cagaatggtg cggcgggacg cggacacggc
     1201 gcgaggatat catcggcgcc gatggccacc cggcccgcac gcctcgcctc ccccgaacct
     1261 ctcgtgggag ccggcctcgc gctcgcggcg atcgccggcg cgatgctgct gcgtcaatgg
     1321 acgctgctgc tggtcccctt tatctggctc gccatcgcgg cgctttcgcg cgccgccctc
     1381 cccttgcgtt tcgcatggct cgccgccgcg acggtgctgc tgccgcgcta tccgcttggc
     1441 ttcgggctct cagtcgacga cctgcttcca tcggccgccg cgatcgccgg cgtcgtgctg
     1501 ctggcacgct ggggcttccc acggctgccg cgctggatgg agtccgcgtt cgcgatctgg
     1561 ctcgtggcgg cggccgcttc cgccgtcgtc aacggccaaa gcctcgggga gatcgcgcgc
     1621 ctcgccggac cgggcgtggg acgtcccgtc ttctggttcg tcttcaccac aacggccgcg
     1681 gcgatcgcgg gccgtctcgg cgtgcgcgcg atcctgatcc ccatcgccgc cgtcgcgatc
     1741 atcgaggccg cgttctcgat cgccgcctac acggtctgcg gcgagattca cctcggcgcc
     1801 gtctcgcggc ccgagaacga gagcgtctgg gacgcgcgac gcgcgctcgg cgtcgagcag
     1861 ggcgccggca cgaacgtcgg cgagcaacgc atccgctgcc gcgccaccgg aacgctcggc
     1921 cagtcctcga acttcctcgc cgccttcctc gtgaccaccc ttcccgtcgc cgcggcgctc
     1981 acgctcgcca agcggcaacg cgcgggacgg cttcgctggg gggccggcac cgtcgcgctc
     2041 gcggctgcac tcgtgctcac cttcacgcgc gcatcgctca tcgccgtcgc gatcgccctg
     2101 ttggtgacgc tgctgctcgc cgcaccgcgc cgcgcgctcc cggtggtggt gatcgcgggc
     2161 ctggtgctcg gcaccgcatc gctggccatc ccgcaggtca agcgccgcct caccgactcc
     2221 gccaacgacc ggcgcgccct gtggtggtcg ggcgtggcga tcttcaagga tcatccgctg
     2281 atgggtgttg gcttcggcaa ctaccgcgag gtgcagcttt cggatcgcaa gtatctcgac
     2341 acgccctacg gcgagccgac ctcgaccgcg cacaacggct tcatcgcgat cgcggcggaa
     2401 ggaggcgtcg ttcaggcagg cgctgtgctc gtgctcgccg gcggcatcct ggtgctgggc
     2461 tggcgcgcaa gccgttcggc gcgaggaacg cccgacgccg cgctcgtcgc cggcgccctc
     2521 ggcggctgcg cgggattcct cgtccagaac atgaccaata cgctgctgct ggtccccacg
     2581 gtcgcgacct acttctgggt gatgggcggc gtcttagccg gcgtcgccgc gaccggcgcg
     2641 ccgccgggcg acgagcgagg ccgcgagcac gccgagatca cggccgaata gcaccgcgta
     2701 caccgccagg acgcccagca cggcgatcat cgccgagccc gtctcgtcga tgcggacctg
     2761 ccacacgatc gccgcaacga tcacccacag catcgcggcc ctgaccacca ccgatgcgat
     2821 ccgcacgcca aaggtgcgca ccgcgaacgc gatcgggatg gccgcttgcg gcaccgatcc
     2881 gatcagatag ccccacgcga caccggtcgc gccgtcccgg gcgcccagcg tcacccacac
     2941 tcccacgccg acgccgaagc cggcgatgct cgcgagcacc ggaaccttgt agtagcggcg
     3001 gtcggttccc gacagcgtcg tcacgtacgg gatctgcacg accgacaaat aggtcgcgcc
     3061 catcatgatc gtcagaatga gcgcgccgtc gtcgaaaccg gccccgaaga gccatcgcag
     3121 gatcaccggt gccgtgaccg cggcggccgc gaccggcgcg agcagcagga tccacagcgc
     3181 gcggcccgtc tcgtgcagtt gccggcgcac gctgtcgagc cgctcctcgc cgtagcggta
     3241 ggcgatcgag gggaagagcg cgagcgagag cgcgcgcgga acgaagtaga cggggattac
     3301 cagggcgagc gccgccgaga acacgcccag cattcgcggc gagtcgtagc gtccgacgaa
     3361 gacgggcgac aagttcaaaa agcccgcgct ggcgacggtc cccgcgatgg ccagtgccgt
     3421 gaagccccgg atctcgcgcc acagcggcgc agggaccggt ttcgcggctc ctcccccgcg
     3481 cagccgcgcc gcggacagga tcgcgaacag cgcgtagccg cccgcgacgg gccacaggat
     3541 cgcgcttcgc gtcgcggcca cggccaccac ggccagcgcg gccagcatcg cgcccgacga
     3601 cagggcctcc agacgcagat acgactccac catgccgaat ccgtaatgcg ccgacttcag
     3661 atgctgatag gcgttgtagg cgaaggcgat cgcaccggcc aggatcgcat cccgcgtcct
     3721 gagcccggcc tcaggaacga cggcgagcac ggcggcgacc aacgcggcaa ccaccacccc
     3781 gcccgaggcg ttgtgcagca gcgttcgccg gtagatcccg gccgcgagcg ctcggtcccc
     3841 cccaccggcc gcctgagcga cgtacttcgc cgtcgaggtc tgccatccgc cgctgaccag
     3901 cagggtcgcg aacatcgcgg cgttgatcgc cagcgtcgcc tcgccgagcg cagccacgcc
     3961 gaagcggcgg cccaccacga tgttgtagac gagccggccc gcacccagca gaccgatcgc
     4021 gaccagcgac agcgcggtgt tccgcgccag ccgtacgtgc gaaggagtgg tttcggtcat
     4081 gggcttgccg gtggttccgg tcatcggtct gccgctcggg ccgtatcata cggccgcacg
     4141 cccgccgatc cggaggccat cgaagagccg tggaacgact gctcgtcgtg tcgaacatgt
     4201 acccgtcgcc gaaggcgccg tacttcggcg tgttcgtcca gcgacaggtg cgcgcgctgc
     4261 aggctcacgg cgtcgacgtg gcgctcgcgg tctcggaccg gcgcggaggc tcggtagccg
     4321 gtgcggcacg caagtacgcg gccctcggcc tcgacgcgca tcgcgtcgcg aagcgctcgc
     4381 atccttgggc ggtgctcgcg catttcgtct ttccgtcggg tgtgatcgcg ctgtccgccg
     4441 gacggaccgc tcgcgctccc gtcgctctcg tcgcgcacgg tggcgacgta gatccgaaca
     4501 agcccgcgtg ggtggcccgc gcgacgcgcg aggtgctcgc gcgcgtcgac cttcttctcg
     4561 ccgtcagccg ctcgatcgcc ggcgaggcac aggccgccgg cgcggatccc gagcgcatcg
     4621 cgatcgcctc gatgggctac gacgccgacg cattcgtgcc gcgcccgcga ccggaagcgc
     4681 gcacgcgact cggcatcccc gaaggccggc agctcgcagt catggtcggc aacctcattc
     4741 cgcgcaaagg cgtgggtgtg atcgtggatg cggccgcggc gctggcgcct gcgcatccgg
     4801 atcttcaatg ggtgctcgtc ggcggcggtg atcaggcgcg gtggcgcgcg caggccgagc
     4861 gtgcgggcag cgcggccgcg atccgcttca ccggaccggc cgatcccgag gacgtcccgt
     4921 ggtggctcag cgccgccgac gtcgccgtcg tgccgtcgct tcgcgagccc tacggcgtcg
     4981 cggcggtcga ggcgctcgcg tgcggcgtgc ccgtcgtcgc ctcgcgcacc ggcggcctgg
     5041 ccgagatcat ggacggcgac cacgggttga ccgtcgagcc cggcgatgcc gcatcgccga
     5101 gtggggcgcc gccgcgacgc taagcgccgg gcggaacgat gtcgccgggc ggaacgatgc
     5161 cgccgggggc ggaggcgggg tccggcggcg gaggcggggt ctcgccgcgt tcctgcgcct
     5221 ggcggcgcgc cagcaccgcc tccttgctcg cgatgcgatg gcgtcggcgg atcttggcct
     5281 cgtcttgacg gcggcgctcc tcgtcggtct cgggatctag gcgcggcacg ggacgcggtt
     5341 ttccttcctc gtcgagcgcg acgaagacca gatacgcgct cgacacgtga cggcgctcgc
     5401 cggtgacggt gttctcggcc tcgacgcgaa cgccaacttc catcgacgtg cggccgacgt
     5461 cgttgacctg cgcggtgagc gtgaccacgt cggtgaggaa caccggctcg atgaagctca
     5521 tctcgtccat cgacacggtc acgacgcgcc catgcgcatg cttgatggcc gacaggcccg
     5581 cggcggtgtc cacgagcttc atgatcacgc cgccatggac gttgcccgcc agattggcgt
     5641 cggtgatgct catcgcctgc gacagcgaca cgcgcgattc gctggggtga cggctttgct
     5701 cgctcatcgg gtcgtaagcg tgtccgaccg cgtagtcgcg cgcaaatctg ccgaggaagt
     5761 cggccacggt cgtgtcgggg gcgagccggc cgctgcgaag ccctaaaagc tcggccatgc
     5821 cgggggcgag ccggcggctt cgaaggttca gaatgtcggc catgccgggg gcgggcggca
     5881 tcacgccggc gccctgaccg gacgcctcgt cgcccgccgg cgagtcctcc cgcggccgtg
     5941 ctcgcatccc cgccgcgatc acccaggcga tgccgaggcc gatcacgatg tcgccgatgc
     6001 tgatgacgtc gcggatcggc cgcacgggga tcacgtcgcc gagccagttc agcaccgttg
     6061 tgtcgctcgc gaagacgtgc ttgagggcga accggttttc ctcgcccgac gggatcgggg
     6121 cgttcgcggc ccgcagcgcc gcgcgcgaga cggggatgcc gcggttcggc gcgatcgcca
     6181 ccaggttcaa cgcggtgccc gcccccacga tcagaaacgc cacgcgcagc caggcgcgat
     6241 gcgcggccgc gaccacgccc agccacacga tgagcaagcc gtaggagccg atcacgatcc
     6301 agaacttcac gtcgcgcgtc agccatgacg ccgagacgaa tcccgcgccc acctgcgcca
     6361 ggaccgcgac gtagagcagc cacacccagg ggaagcgaac gctcgccgcg agccatcgca
     6421 gcctgccgcg catcgcgaag cctgcgagca ggcccagcgc gatcccggcg agatacagca
     6481 tcgtgcgtcg attctaggcg cgacggctca tcgcgccccg gcagccgggc acgcactaga
     6541 ctcggccccc gatgagaagc cgattccgcc cggccgtcgt cgcggccatg ctcgccgccg
     6601 tcgtcggtgg cattgccgca cccgcacccg catcgtcgat cgacgccggc ggcaggctcg
     6661 tggtcgcggt cgagctgaag gcgatgcgcg gcgacgccgt gaccgtctgc caggaccagc
     6721 cgttcggcga gggcgtggag acgttcgccg acacggtcgc ggccgcattc ggcttgccgc
     6781 tgaccggcgt caacgcctcg ccgtcttcgg attgcttcgc gctgaatctg acgggtccgc
     6841 tgaccacgcg cggccccggc ggcgcgcgca tccttcgctt cgatgccggc gcgctgtcct
     6901 cgatcgcgag cgaggcgggc ttcggcgagg cgttcctggt cgtgtgcacc ccgaacgtga
     6961 ccgaccggat ccaggtgctg accggcgacc gtccggcccg cgcgagcggc tgcgacgcga
     7021 agggctacgc gtggagcctg gccgatccac tcgagatcga ggttgccttc cgtgcgaccg
     7081 gagccgacct ggccgaaggg ctgctcgcgg ccgcagcgtg gtggatcgcg ttcgccacgc
     7141 tgctcggcat cgcagtcgtg cgtcgcgccc agctcgggct gctgcgccgg atgccactgc
     7201 tcggcggcgc cgccggcgcg ctcgtcgccg gcgcggccgc ctatggatgg atcttcgtcg
     7261 cttcgcgctc cccgctcgtg gacgcgctgg cgatgctcgc cggcctgggc tcggccggtg
     7321 aggttgcgct gctggcgagc ggctcggcgc tggcgctcgc gctcaccgcg gtcggggcgc
     7381 gccgcat
//
LOCUS       DATLFO010000303         5234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_77253, whole genome shotgun sequence.
ACCESSION   DATLFO010000303 DATLFO010000000
VERSION     DATLFO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77253"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VM841_12290"
     CDS             <1..1086
                     /locus_tag="VM841_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HVL91003.1"
                     /translation="ILDLQTQAITRALDDCGLTLEDVDGLLTTGVSRFSASQVAEYLG
                     IYPTWTDSTMAGGSSFEIFAAHAAEAIRTGRCEVAVISYGSNQRSARTRTLGGVIDPH
                     IPYAQFEEPYGPLNPISMYALAAQRYMHEFGTTSEQLAEVAVAARAWALKNPKAYRHG
                     DGPLTVDAVLASPTISSPLHALDCCLVVDGGGAVVMTTAERARDLRTRPVTVLGHGET
                     TTHVGITQMPDLTSTGATISAARAYEMAGLAPADVDVAQVYDSFTITVLLTLEALGFC
                     ARGEAGAFVEKGRIAPGGERALNTNGGGLSYCHPGMYGVLLLVEAVRQLRGECGERQV
                     PGAEVAICHGTGGILSTHATVILGAAR"
     gene            1083..1478
                     /locus_tag="VM841_12295"
     CDS             1083..1478
                     /locus_tag="VM841_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HVL91004.1"
                     /translation="MTKPVAPPDNVTQPWWDATREERLLVQRCEGCGHAQLYPRSSCT
                     ACHGTELSWAEASGRGTVYSHTTVHRAPSDAFTAPYVVLLVRLEEGPVVMANLAGQGE
                     VRCDQPVEAVWEDLPDGRKLLQFAAASRT"
     gene            complement(1479..2078)
                     /locus_tag="VM841_12300"
     CDS             complement(1479..2078)
                     /locus_tag="VM841_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91005.1"
                     /translation="MRSKGVRFLAAGAAVAALAAAPARAGEDATVWHELRSARAIASW
                     GAVSLDVAAYAGDGVHIVQGTPEAVSFATVCVKVRDRARRRFEHGCGSVPLTFDAITG
                     TASIRGSVPTNVLVLNQYKILALSSITVRAELGGAEAWRPGPVFVPALSDGRLMAPVS
                     GVWRPARATISFRSAVLGAYFSRTTAQSSIGRSAAVVAG"
     gene            2202..3365
                     /locus_tag="VM841_12305"
     CDS             2202..3365
                     /locus_tag="VM841_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL91006.1"
                     /translation="MDFELSEEQTMFRKVIRDFVGDHIKPVAREWEHAGRYPTEIVDK
                     MKEMGLFGVTVPEEFGGMALDMVSMAIVFEEISKGWMGVAGTIGSHSLSCRMIAAHGT
                     DEQKHRWLPRLATGELRTGIGLTEPGAGSDLQGIATRAMRDGDSYVVRGTKTWITNAR
                     HAGILPVLVKTDPDAQPRHTGMSMLVVDMSSPGVSVSRDIGKLGYKGTESCEVVFDDV
                     RVPEHDLLGGVEGVGLKQALSGLEAGRINIAARAVGIATAALEAALEYSQQRHAFGNP
                     ISDFQAIQLKLADMGTETTAARLLTWWAASRLDAGKRADAEAGMAKLFASEVAIKASL
                     ESMRIHGGYGYSTELDIERLYRDAPLMAIGEGTNDILRLVIAHRLLKDGMPLA"
     gene            3362..4156
                     /locus_tag="VM841_12310"
     CDS             3362..4156
                     /locus_tag="VM841_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVL91007.1"
                     /translation="MRSCLYVPGDKPEMLAKAASRGADVIMADLEDAVPPGRKLEARE
                     SVIEFLRSDARRGASIWVRINDEADAHAIAPLRPDGISVPKVACVADIERVDVDLPVI
                     ALIETADGVIDAREIARHPSVVRLALGEADLGADLGIDPSPDHHEWQAVRTMIVLASA
                     AAGIEAPVAPVTADFTDLVALRASTVALKRMGFGARAAIHPAQVPVINEVFTPTADEI
                     AAAQRLLQQFESAGGGVTTDDRGHMIDEAIVRSARRTLSRAPKHDQ"
     gene            4321..4821
                     /locus_tag="VM841_12315"
     CDS             4321..4821
                     /locus_tag="VM841_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91008.1"
                     /translation="MIKKLALAAVAASIALSGAQGAFAHRDTTPDTGNRCDTWSRPGH
                     GTGDSHHHTGSADINGGVVFVHQHGGHYVVRNDNAYVEVVGGQGYRGPSPTGQMNPGQ
                     GGYVQGEADLGIAGVPDADFHANVFSPDADHPPALTPQAVQTWANGYNYLACLNVANS
                     RIETKK"
     gene            4930..>5234
                     /locus_tag="VM841_12320"
     CDS             4930..>5234
                     /locus_tag="VM841_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91009.1"
                     /translation="MKRISGERIKATLNHQWRGLGAFIAVVLLVNVLVAVRDAAQTSQ
                     PAAAPGSEGTGEIPSDPGFDAGVPTVIPTTSPGSGPGSLPNGPGGIKSLPPGVKPGSA
                     "
BASE COUNT          865 a         1850 c         1791 g          728 t
ORIGIN      
        1 atcctggacc ttcagaccca ggcgatcaca cgagcgctcg acgattgcgg actgacgctc
       61 gaggacgtcg acgggctgct gaccaccggc gtgtcgcgct tcagcgcctc gcaggtcgcc
      121 gagtatctgg gcatctatcc gacgtggacg gactcgacga tggccggcgg ctcgtccttc
      181 gagatcttcg ccgcgcacgc ggccgaggcg atccggaccg gccgctgcga ggtcgcggtg
      241 atctcctacg gcagcaatca gcgctcggcc aggacgagaa cgctgggcgg ggtcatcgat
      301 ccgcacatcc cctacgcgca gttcgaggag ccctacgggc cgctcaaccc gatctcgatg
      361 tacgcgctcg ccgcgcagcg gtacatgcac gagttcggca cgacctccga gcagctcgcc
      421 gaggttgcgg tcgcggcccg ggcctgggcg ctgaagaacc cgaaggcgta tcgccacgga
      481 gacggtccgc tgaccgtgga cgcggtgctc gcctcgccga cgatctcctc gccgctgcac
      541 gcgctcgact gctgcctggt cgtcgacggc ggcggcgcgg tggtgatgac caccgccgag
      601 cgtgcccgcg atcttcgcac caggccggtg acggtgctcg gccacggcga gacgaccacg
      661 cacgtcggca tcacgcagat gcccgacctg acctcgaccg gcgcgacgat cagcgcggca
      721 cgcgcctacg agatggcggg cctggcgccc gccgacgtcg acgtcgcgca ggtctacgac
      781 tcgttcacga tcacggtgct gctcacgctc gaggcgctgg gcttctgcgc gcgcggcgag
      841 gcgggagcgt tcgtcgagaa gggccgcatc gcgccgggcg gcgagcgtgc gctcaacacc
      901 aacggcggcg gcctgtccta ctgtcacccc ggcatgtacg gcgtgctgct gctcgtcgag
      961 gcggtgcgcc agctccgcgg cgagtgcggc gagcgacagg tgcccggtgc cgaggtcgcg
     1021 atctgccacg gcaccggcgg aatcttgagc acccacgcga ccgtgatcct gggagcggcg
     1081 cgatgaccaa gccggtcgcg ccgcccgaca acgtcacgca gccgtggtgg gacgcgacgc
     1141 gcgaggaacg cctgctcgtt cagcgttgcg aggggtgcgg acacgcgcag ctgtatcccc
     1201 ggtcctcgtg caccgcctgt cacgggacgg agctgtcgtg ggccgaggcg agcgggcgcg
     1261 gcaccgtgta ctcgcatacg accgtgcatc gcgcgccgag cgacgccttc accgcgccct
     1321 acgtcgtgct gctggtccgc ctcgaggaag ggcccgtcgt catggccaat ctcgccggcc
     1381 agggcgaggt gcgctgcgat cagccggtcg aagcggtgtg ggaggatctg ccggacggac
     1441 gaaagctgct gcagttcgcg gccgcgagcc ggacctgatc agccggcgac gacggcggcg
     1501 ctgcggccga tcgagctttg ggccgtggtc cgcgagaagt aggccccgag caccgcggag
     1561 cggaacgaga tcgtggccct cgccgggcgc cacacacccg acaccggcgc catcagccgt
     1621 ccgtccgaca gcgcggggac gaagaccgga ccgggccgcc aggcctcggc gccgccgagc
     1681 tcggccctga ccgtgatcga agacagcgcg aggatcttgt actggttcag cacgagcacg
     1741 ttggtcggga cgctgccgcg gatcgacgcg gtgcccgtga tcgcgtcgaa ggtcagcggc
     1801 accgatccgc acccgtgctc gaagcggcgg cgcgcgcgat cccgcacctt tacgcagacc
     1861 gtggcgaacg aaaccgcctc cggcgtcccc tgcacgatgt gcaccccgtc cccggcgtag
     1921 gcggcgacat cgagcgacac cgcaccccac gaggcgatgg ccctcgccga gcgcagctcg
     1981 tgccagacgg tcgcgtcctc tcccgcacgg gcgggagcgg cggccagcgc ggccacggcc
     2041 gcaccggccg cgagaaagcg aacgcccttc gatctcatac atctcgaaac gaaccggcac
     2101 cgggaagggt tacgcatatt gtcccgcgtc gggcgttcct gccggcgaaa accccggtgg
     2161 tacgctggcc gcgcccgcac cggccgacgg agccttcgac catggatttc gagctcagcg
     2221 aagaacagac gatgttccgc aaggtgatcc gcgacttcgt cggcgatcac atcaagcccg
     2281 tcgcccgcga atgggagcac gccggccgct accccaccga gatcgtggac aagatgaagg
     2341 agatgggcct gttcggggtg accgtgcccg aggagttcgg cggcatggcg ctcgacatgg
     2401 tctcgatggc gatcgtgttc gaggagatct cgaaaggctg gatgggcgtc gccggtacga
     2461 tcggctcgca ttcgctgtcg tgccgcatga tcgccgccca cggcaccgac gagcagaagc
     2521 accgctggtt gccgaggctg gcgaccggcg agcttcgcac cggcatcggc ctgaccgagc
     2581 cgggtgcggg atcagacctg cagggcatcg cgactcgcgc gatgcgcgac ggcgattcct
     2641 acgtcgtgcg cggcaccaag acctggatca ccaacgcgcg gcacgccggc atccttccgg
     2701 ttctggtcaa gaccgacccc gacgcgcagc cgcggcacac gggcatgagc atgctggtgg
     2761 tggacatgtc ctcgccgggc gtgagcgtga gccgcgacat cggaaagctc ggctacaagg
     2821 gaaccgaatc ctgcgaggtc gtcttcgacg acgtgcgagt cccggaacac gatctgctcg
     2881 gcggcgtcga aggggtcggg ctcaagcagg cgctgtcggg cctggaagcc ggccgcatca
     2941 acatcgccgc gcgcgcggtc ggcatcgcga ccgccgcgct cgaggccgca ctcgagtact
     3001 cgcagcagcg tcatgcgttc ggcaatccga tcagcgactt ccaagccatc cagctcaagc
     3061 tcgccgacat gggcaccgag accaccgccg cgcggctgct cacctggtgg gcggcttcgc
     3121 gcctggacgc gggcaagcgc gccgatgccg aggcgggcat ggcgaagctg ttcgcatccg
     3181 aggtcgcgat caaggcgtcg ctcgagtcga tgcgcatcca cggcggctac ggctactcga
     3241 ccgagctcga catcgagcgg ctctatcgcg acgcgccact gatggcgatc ggcgagggta
     3301 ccaacgacat cctgcgcctg gtcatcgcgc accggctgct caaagacggg atgccgctcg
     3361 catgaggtcc tgcctgtacg tgcccggcga caagcccgag atgctcgcga aggcggcctc
     3421 gcgcggcgcc gacgtgatca tggccgatct cgaggatgcc gtgccgcccg gccgcaagct
     3481 cgaggcgcgc gagagtgtga tcgagttcct tcgcagcgac gcacgccgcg gcgcgagcat
     3541 ctgggtgcgt atcaacgacg aagccgacgc gcacgcgatc gcgccgctgc ggcccgacgg
     3601 gatctcggtg cccaaggtcg cctgcgtcgc cgacatcgag cgcgtggacg tggaccttcc
     3661 ggtcatcgcg ctgatcgaga ccgccgacgg cgtaatcgac gcgcgcgaga tcgcccggca
     3721 cccgtcggtg gttcgcctcg ccctcggcga ggccgacctc ggcgccgatc tcggcatcga
     3781 tccgtccccc gatcatcacg agtggcaggc ggtgcggacc atgatcgtgc ttgcctcggc
     3841 cgcggcgggg atcgaagcgc ctgtcgcgcc ggtcacggcc gacttcaccg acctcgtcgc
     3901 gctccgcgcc tcgaccgtcg cgctcaaacg catgggcttc ggtgcgcgag ccgcgatcca
     3961 tcccgcgcag gtgccggtga tcaacgaggt cttcacgccg accgccgacg agatcgccgc
     4021 cgcgcagcgg ctgctccagc agttcgagtc ggcgggcggc ggcgttacca ccgacgaccg
     4081 cggccacatg atcgacgagg ccatcgtccg gtcggcccgg cggacgctgt cccgcgcacc
     4141 caagcacgac cagtagcgcg gccggcaagc gctgcggggc gacgcgactc agacatcgaa
     4201 tcggacatca tggtacgttc gatctcgtag cggtgtttcg cgcagcgaaa cggcaggagt
     4261 ccggcttgct cgcggcgaac cggtgtatca ccggcccgtc cgcgaaaggg gaggcacacg
     4321 atgatcaaga agctcgcact cgcggcggtc gccgcatcga tcgcgctgag cggcgcccag
     4381 ggagcattcg cccaccgtga caccaccccc gacaccggca accgctgcga cacgtggtca
     4441 cgtcccggac acggaaccgg cgacagccac catcacaccg gaagcgcgga catcaacggc
     4501 ggcgtcgtct tcgtgcacca gcacggcggc cactacgtcg tgcgcaacga caacgcctac
     4561 gttgaggtcg tcggcggaca aggctatcgc ggcccgtcgc cgaccggaca gatgaacccc
     4621 ggccagggtg gctacgtcca aggtgaggcc gatctcggca tcgccggcgt tcccgacgcc
     4681 gacttccacg ccaacgtctt ttctcccgac gcggatcatc cgccggcgct gacacctcag
     4741 gccgtccaaa cctgggcgaa cggatacaac tacctcgcgt gcctgaacgt cgcgaactca
     4801 cgaatcgaga cgaagaagta ggcgacaaac tgtcgtgaaa cgggacgaga tcgcccggcg
     4861 agtggcgatc tcgtctcgca tttatctctc atatactgcg gagacgcagc acagagggag
     4921 gatgggccgg tgaagcgcat ctcgggcgag cggatcaagg ccacgctgaa ccatcagtgg
     4981 cgcggcctcg gcgccttcat cgccgtcgta cttctggtca acgtgctcgt cgccgtacgc
     5041 gacgccgcgc agacgtcgca gcccgccgcg gcccccggct ccgagggaac cggcgagatc
     5101 ccgagcgatc ccggcttcga tgcgggtgtc ccgaccgtca tcccgaccac atcgccgggg
     5161 agtggccccg gctcgctgcc gaacggcccc ggcggcatca agtcgcttcc gccaggcgtg
     5221 aagcccggca gcgc
//
LOCUS       DATLFO010000304         6964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_38328, whole genome shotgun sequence.
ACCESSION   DATLFO010000304 DATLFO010000000
VERSION     DATLFO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38328"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..839)
                     /locus_tag="VM841_12325"
     CDS             complement(18..839)
                     /locus_tag="VM841_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HVL91010.1"
                     /translation="MDASIVIVGDEILSGHVREANAHFIATRLAAHGHALRRIAIVPD
                     EPREIAASVAREVGDPAVDIVFVCGGLGPTHDDRTMEGVAIALDVGLESCAPIAKRIA
                     EIAGHVHAEAFDGDPLGIGGLEKMALAPAGSVALECASGVIPAVTTEAGDTRLFILPG
                     PPRELGAVFTECVEPTYLEGTGTIPVREEILHEFPESALAVVLAEVEASIPVKIGSYP
                     GTERVLVRIAGAAEPVAAAAKRIREAIDTLAASTDGQRLLALMRRRREERARERG"
     gene            complement(865..2541)
                     /locus_tag="VM841_12330"
     CDS             complement(865..2541)
                     /locus_tag="VM841_12330"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL91011.1"
                     /translation="MSPPVPARPRLGRLIRDRAAVASSATRQVMGHRSLRSLEASLLV
                     NWTAEHAYLVALGVYAYSVGGALAVGLLGLGRLLPAGLVALMASAAADRYPREVVLRL
                     VYAVRALAAIASAAAFFAGAPPTLVLVLGGSVTVLSAALRPAYWALLPMLATSPEQLA
                     ASNVLASTSEGAALLVGPLIGGLLVAGTDPGTVFVAAGQMFLISVFFSMLVVSDARPA
                     PVEERGLFDRTIEGMRAVAAHHDTRFLVVIGGAQTFVRGALTVLIVIAALDLLAMGEA
                     GVGYLNAAFGIGNLVGAVAALAFVGRRRLGGPLLGGLLMWGAPILLMGVFPSPIAAFA
                     LLLIPGWGNAVFDIAILTMLQRITPDEVLGRVFGILETAVFVAMAAGSIAASGLVAWL
                     GVRGALIVCGSLLPVLVIVTRARVRAIDDRSEVPEREIGLLRHLPLFAGLPAVALEHL
                     AAKLTPVAVGSGDVVFRQGDPGDRFYVVGDGQVIITKQARDVASLGRGDYFGEIALLR
                     GIPRTATVTASTPTELLALAPEDFLAAVSRYAQCARRADQEMARRLDDVV"
     gene            complement(2538..3248)
                     /gene="bshB1"
                     /locus_tag="VM841_12335"
     CDS             complement(2538..3248)
                     /gene="bshB1"
                     /locus_tag="VM841_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HVL91012.1"
                     /translation="MNAFDIVAFGAHPDDAEIGCGATLVMAAAAGRRVAIVDLTAGEQ
                     ATRGSVEEREKERRRAAETLGIDTRPCLCLPDAALGSDPVHRDRIVEIMRDLRPRIVL
                     APMAPDRHPDHAAAGRLVAEASYLSGLSTGSPPQRPARVYHYMIHHPITPSFVVDAAP
                     GWAQKMAALRCHESQFDDREPRTAISGGSFLEVIDARGVYFGSLIGAARGEPFWSAGP
                     VPQASLPDITADGYGAYP"
     gene            complement(3245..4270)
                     /locus_tag="VM841_12340"
     CDS             complement(3245..4270)
                     /locus_tag="VM841_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91013.1"
                     /translation="MTTVSLPPSVHADLTSFCAALRPPAAGGPVRVYCGHQIQADYPA
                     SVVAKLAALAGLAEAGPVALWLDTDRAGSQRAMTTITLPDGTRIPWVPHRVRDAESRF
                     IPLGIDRVAGIADALREWARVQAPGARSRLDALVAAAASAPQTLSSALRAWSMHLVRA
                     GLGLAAGDVMVSDLLDRPAVRARVQDLVDSIEDVVAVFNETVESLVAGGLDPAVRPVA
                     PDYLPLHYSCPVDGSRRRLSRRGRDAEAECGCGAVYRFSLGDLDALFATRRWSTDVTL
                     PLAIEPLYSGVVAGKTSWRYGLVLGAVGRRVWQSEPLPSFLPPALTGDGDAAGETTLL
                     LRYLGDR"
     gene            complement(4267..5385)
                     /locus_tag="VM841_12345"
     CDS             complement(4267..5385)
                     /locus_tag="VM841_12345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91014.1"
                     /translation="MRVGIVCHHGFGGSVRTAVHLACALARRGHGVDLLSRRVPLGSA
                     RLGPGVTVHALAVTGGPSAILQTEWPARELSDFEAMIAGADARGTDVIHFHYGLPFAE
                     IVSSLRPSLRAALVGTFHGTDVETAERDASVARRLRAAARDLDAVTTVSRAYAARVRR
                     VLGVDPIVIPNFVDLDVFRPALQRTRTRPVIVHVSNFRPVKDPESLVRVGARAMAAVD
                     AELWLVGDGPGLATVRTELARRGLRDRVRAFGLQADVAPLLERSDVMLLTSRAESFSI
                     AALEAAAAGLAVVAPRVGGIDEVVRDGATGILYEPRDETAAVAALTMLLSDEPVRARM
                     GRAARARAAAFRVDAIVPRYLNLYRAVLSAGVLREAAR"
     gene            complement(5388..>6964)
                     /locus_tag="VM841_12350"
     CDS             complement(5388..>6964)
                     /locus_tag="VM841_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVL91015.1"
                     /translation="AMRAVVEADVDGAGVSVVRPVTFPAAFPGGARALPPAIVPPIGL
                     RPAEHRRLLPSGERPRRIEMVVESEGFDDDAPAATLHCEVPGGWSVHPPSYEATCAAG
                     RTAAYRFEIVVPAGAAGREVVRFRAETGGRSYDGGLEPVRRTDSFLPADASTAVMEAF
                     MPVRAHVEVLVANATFDTTLRYGYVRGDEPSLARALSEFGLEIAELTDEQIALADLSA
                     FDAIVIGPRGYAVRPTLTAHAPRLTACVEAGGTVITLAQSYGYDSAGSAPLAIRYAHP
                     HDRVTAPDAPVTILEPAHAIFSAPNRITAADFGGWSGERGRYFAAEWDPRLVPLLACA
                     DDGEVPARGGLLAGGLGRGTFVYCGYSLARQIDAGVEGAIRLLANLLALPESRIREKM
                     EHLGRVPLFAGLTGQELHRVARLASQRWFAPGELLCRQGDRSEELYVVTGGSVDVLKE
                     SGRAIATAGVGSVIGELAVISEAPRSATLRARDGVDTLVLRGAHLRELLRELPDMSER
                     MMTILAAKLAADRPGP"
BASE COUNT         1122 a         2530 c         2489 g          823 t
ORIGIN      
        1 agcccccagc tctgccgcta gcccctttcg cgggcgcgtt cttcgcggcg gcggcgcatc
       61 agcgccagca gtcgctggcc gtcggtggat gcggcgagcg tgtcgattgc ctcgcggatg
      121 cgcttcgccg cggccgcgac cggctcggca gcaccggcaa tacgcacgag cacccgctcg
      181 gtgccgggat acgagccgat tttgacgggg atcgacgcct cgacctcggc caggacgacg
      241 gcgagcgcgg actccgggaa ctcgtgcagg atctcctcgc gaaccgggat cgtgccggtc
      301 ccttccagat aggtcggctc gacgcattcg gtgaagacgg cgccgagctc gcgcggcggg
      361 ccgggcagaa tgaacagccg ggtgtcaccc gcctcggtcg tcacggccgg gatcacgccg
      421 gacgcgcact ccagggccac gctgcccgcg ggagcgagcg ccatcttctc gagcccgccg
      481 atgccgagcg ggtcgccgtc gaaggcttcg gcgtgcacgt gtccggcgat ctcggcgatg
      541 cgcttcgcga tgggcgcgca cgactcgagg cccacgtcga gcgcgattgc gacgccctcc
      601 atggtccggt cgtcgtgcgt tggaccgagt ccgccgcaaa cgaagacgat gtccaccgcg
      661 ggatcgccga cctcgcgcgc gacgctcgcg gcgatctcgc gtggctcgtc tgggacgatc
      721 gcgatgcggc gcagcgcgtg gccgtgtgcg gcgagccgag tcgcgatgaa atgcgcgttg
      781 gcttcgcgga cgtgacccga gaggatctcg tcgcccacga tcacgatgga ggcgtccatg
      841 ctcgcgacag catacgaccg cggctcacac gacgtcatcg agccggcggg ccatctcctg
      901 gtccgcgcgc cgcgcgcact gcgcgtagcg gctgaccgcc gccagaaaat cctccggcgc
      961 gagcgcgagc agctcggtcg gcgtggaggc ggtcacggtc gcggtgcgcg ggatgccccg
     1021 cagcagcgcg atctcgccga aatagtcgcc gcgcccgagc gaggcgacgt cgcgcgcctg
     1081 cttggtgatg atcacctgcc cgtcccccac gacgtagaac cggtcgcccg gatcgccctg
     1141 ccggaatacg acgtcgccgg agcccacggc gaccggtgtc agctttgccg caaggtgctc
     1201 gagcgcgacg gccggcaagc cggcgaacag cggcagatgg cgcagaagcc cgatctcgcg
     1261 ttcgggcact tcggagcgat cgtcgattgc gcgcacgcga gcgcgggtca cgatcacgag
     1321 cacgggaagc agcgagccgc acacgatcag cgcgccgcgc acgccgagcc acgcaaccag
     1381 accggaggcg gcgatcgatc cggcggccat cgcgacgaag acggcggtct ctaggatgcc
     1441 gaacacgcgg ccgagcacct cgtcgggcgt gattcgctga agcatcgtca gaatggcgat
     1501 gtcgaagacc gcgtttcccc atccgggaat cagtagcaac gcgaacgccg cgatcgggct
     1561 ggggaacacg cccatcagca ggatcggcgc gccccacatc agcagccctc cgagcaacgg
     1621 tccgcccagg cggcggcgcc ccacgaatgc gagcgcggcg acggcaccca cgaggttgcc
     1681 gatgccgaac gcggcgttga gatatcccac gcctgcctcg cccatggcga gcagatcgag
     1741 cgcggcgatc acgatgagca cggtgagcgc gccgcgcacg aacgtctgcg cgccgccgat
     1801 cacaaccagg aagcgcgtgt cgtgatgcgc ggcgacggcc cgcatcccct cgatggtccg
     1861 gtcgaacagt ccgcgttcct ctaccggtgc gggccgggcg tcggacacga ccagcatcga
     1921 gaagaagacc gatatcaaga acatctgacc ggccgcgacg aagacggtcc ccggatcggt
     1981 gccggcgacg agaaggccgc cgatgagcgg accgaccagc aacgcggcgc cttcgctggt
     2041 gctcgcgagc acgttcgagg ccgcaagctg ctcgggcgag gtcgcgagca tcggcagcaa
     2101 ggcccagtac gcgggacgca gcgcggcgga cagcaccgtg accgagccgc ccaggacgag
     2161 cacgagcgtg ggcggggcac cggcgaagaa cgcggccgcc gacgcgatcg cggccagggc
     2221 ccgcaccgcg tacaccagcc ggagcacgac ctcgcgcgga tagcggtccg ccgccgccga
     2281 cgccatcaac gcgacgagcc ctgcgggcag cagccggccg aggccgagca gcccgacggc
     2341 gagcgcgccg ccgacggagt aggcgtagac gcccagggcg acgagatagg cgtgctcggc
     2401 ggtccagttg accagcagcg acgcctccag gcttcgcagc gagcgatgcc ccatgacttg
     2461 acgggtcgcc gacgacgcga cggcggcgcg atcacggatc aagcgtccga gccgcggccg
     2521 cgcgggcacg ggcgggctca cggatacgcc ccgtagccgt cggccgtgat gtccggcagg
     2581 gatgcctgcg ggaccggccc ggccgaccag aacggctcgc cccgcgcggc tccgatgagc
     2641 gagccgaagt acacgccgcg cgcgtcgatc acctcgagga acgagccccc cgagatcgcg
     2701 gtccgaggct cgcgatcgtc gaactgcgat tcgtggcaac gcagggcggc catcttctgc
     2761 gcccatccgg gcgcggcgtc caccacgaac gacggcgtga tcggatgatg gatcatgtag
     2821 tgatagacgc gcgccgggcg ctgcggcggc gagccggtgg aaagccccga cagatacgac
     2881 gcttcggcga cgagccggcc ggcggcggca tgatcgggat gacggtccgg cgccatcggg
     2941 gcgagcacga tgcgcggccg caggtcgcgc atgatctcaa cgatccggtc ccgatggacg
     3001 gggtcggagc cgagcgcggc gtccggcagg caaaggcacg gccgcgtgtc gatgccgagc
     3061 gtctcggccg cgcgccggcg ctccttctcg cgttcctcga ccgagccgcg cgtcgcctgc
     3121 tcccccgcgg tcagatccac gatcgcgacg cggcggccgg cggctgcggc catgaccagc
     3181 gtcgcgccgc acccgatctc ggcgtcgtcg ggatgcgcgc cgaacgcaac gatgtcgaat
     3241 gcgttcatcg atcacccagg taccgcaaaa gcaaggtcgt ctcacccgcc gcgtcaccgt
     3301 cgccggtcag cgccggcggc aggaacgacg gcagcggctc gctttgccaa acccgccggc
     3361 cgaccgcccc cagcaccagg ccgtatcgcc aggacgtctt gcccgcaacc acgcccgagt
     3421 agagcggctc gatcgccagc ggcagcgtga cgtcggtgct ccatcggcgc gtggcgaaca
     3481 gcgcgtcaag gtcgccgagc gagaaccggt acacggcgcc gcagccgcac tcggcctccg
     3541 catctcgccc gcggcggctc agccgccggc ggctgccgtc caccggacac gaatagtgca
     3601 gcggcagata gtccggcgcg accggccgca ccgccgggtc caatccgccg gccacgagcg
     3661 attcgacggt ctcgttgaac acggcaacga cgtcctcgat cgaatcgacg agatcttgca
     3721 cacgcgcgcg caccgccggc cggtcgagca gatccgacac catcacgtcg ccggccgcga
     3781 gcccgagccc ggcacggacc agatgcatcg accaggcgcg cagcgccgac gacagcgtct
     3841 gcggcgcgct cgccgcggcc gcgaccagcg cgtcgaggcg agaacgcgcg ccgggtgcct
     3901 ggacgcgagc ccactcgcgg agcgcatcag cgatcccggc gacccggtcg atgccgagcg
     3961 ggatgaagcg cgattcggcg tcgcgcacgc gatgcggcac ccacgggatg cgcgttccgt
     4021 cgggcagcgt gatcgtggtc atcgcgcgct gcgagcccgc ccggtcggtg tcgagccaca
     4081 gcgccaccgg acccgcctcg gcaaggcctg ccagcgccgc gagcttggcg acgaccgatg
     4141 ccgggtagtc ggcctgaatc tgatggccgc agtacacgcg gaccggaccg ccggcggcgg
     4201 gtggccggag cgcggcgcag aacgaggtga ggtcggcgtg gaccgacggt gggagcgaga
     4261 ccgtcgtcat ctcgcggcct cacgcaggac gccggcgctg agcaccgcgc gatataggtt
     4321 caggtatcgc ggcacgatcg catcgacccg gaacgcggcc gcacgcgctc gcgccgcgcg
     4381 gcccattcgc gcgcgaacag gctcgtccga cagaagcatc gtgagcgcgg caacggcggc
     4441 cgtctcgtcg cgcggctcgt agaggatccc ggtcgcaccg tcgcgaacga cctcgtcgat
     4501 accaccgacg cgaggggcca cgaccgcaag cccggccgcg gcggcttcga gcgcggcgat
     4561 cgagaagctt tcggcccggc tggtgagcaa catcacgtcg ctgcgctcga gcagcggcgc
     4621 gacatcggcc tgcaggccga aagcgcgcac ccggtctcgc aggccgcgcc gagccagctc
     4681 ggttcgcaca gttgcgagcc cagggccgtc cccgaccagc cacagctcgg catcgacggc
     4741 cgccatggca cgagcgccca cgcgcacgag cgactcggga tccttgacgg gccggaagtt
     4801 cgacacgtgc acgatgacgg gccgcgtccg cgttcgctgc aaggcgggac ggaagacgtc
     4861 gagatcgacg aagttcggga tcacgatcgg atcgacgccg aggacgcggc ggacgcgcgc
     4921 ggcgtaggcc cgggacacgg tcgtgaccgc gtcgagatcg cgcgccgccg cgcgcaggcg
     4981 ccgcgccacc gacgcgtccc gctcggcggt ctcgacgtcg gttccgtgga aggttccgac
     5041 gagcgcggcg cgcagcgacg gccgcagcga cgacacgatc tcggcgaacg gcagcccgta
     5101 gtgaaagtgg atcacgtccg ttccgcgagc gtccgcgccg gcgatcatcg cctcgaagtc
     5161 gctcagctcc cgagcgggcc actcggtttg aaggatcgcg ctcggtcccc cggtgaccgc
     5221 cagcgcatgg acggtcacgc cgggaccgag ccgcgccgag cccagcggca cccgccgcga
     5281 cagcaggtcc acgccgtggc cacgccgggc gagcgcgcac gccagatgca cggccgttcg
     5341 cacgctgccg ccgaatccgt gatggcacac gatgccgacg cgcatggcta cggtcccggc
     5401 cggtcggccg cgagcttggc ggcaagaatc gtcatcatgc gttcgctcat gtcgggaagc
     5461 tcgcgaagca gctcgcgcag atgcgcgccg cgcagcacga gcgtgtcgac gccgtcgcgc
     5521 gcgcgcagcg tcgccgaccg cggtgcctcg gatatcaccg cgagctcgcc gatcacgctg
     5581 ccgacacccg cggtggcgat cgcccggccg gactctttga gaacgtccac gcttccgccg
     5641 gtgaccacgt agagctcctc ggagcggtct ccttgccggc acagcagctc gcccggcgcg
     5701 aaccagcgct gcgaggcgag ccgcgcgacc cgatgcagct cctgcccggt cagcccggcg
     5761 aacagcggga cccggcctag gtgctccatc ttctcgcgga tacggctctc gggcagggcc
     5821 aaaaggttcg cgagcagacg gatggctcct tccacgccgg catcgatctg acgcgcgagc
     5881 gagtagccgc agtacacgaa ggtgccgcgc cccagcccgc cggcgagcag cccgccgcgc
     5941 gccggcacct cgccgtcgtc cgcgcacgcg agcagcggaa ccagccgcgg atcccactcg
     6001 gccgcgaagt agcgcccgcg ctctcccgac cagccgccga agtcggctgc ggtgatccgg
     6061 ttcggcgcgg agaagatcgc gtgtgcgggc tcgaggatcg tcacgggcgc atccggtgcg
     6121 gtgactcgat cgtggggatg cgcgtagcga atggccagcg gggccgagcc tgcgctgtcg
     6181 tacccgtagg actgcgcgag cgtgatcacc gttccgcccg cctcgacgca ggcggtgagc
     6241 cgcggcgcgt gtgcggtgag cgtgggccga accgcgtacc cgcgcggccc gatcacgatc
     6301 gcgtcgaagg cggacagatc ggcgagggcg atctgctcat cggtcagctc ggcgatctcg
     6361 agcccgaact cgctcaacgc gcgggcgagc gacggctcgt ccccccggac gtacccgtac
     6421 cggagcgtgg tgtcgaaggt cgcgttcgcg acgagaacct cgacgtgggc gcgcacgggc
     6481 atgaaggctt ccatcacggc cgtcgaggca tcggcgggaa ggaaagagtc cgttcggcgg
     6541 acgggttcga gcccgccgtc atagctgcgg ccgcccgtct cggcccggaa gcgcaccacc
     6601 tcgcggcccg cagcgccggc gggcacgacg atctcgaagc ggtacgcggc cgtgcgtccc
     6661 gcggcgcagg tcgcctcgta ggacggcgga tgaacgctcc agccgccggg aacctcgcag
     6721 tgcagcgtcg cggccggcgc gtcgtcgtcg aagccttcgc tctcgacgac catctcgatg
     6781 cggcgcggcc gctcgccgga cggcaggagc cgccggtgct ccgccggccg caaaccgatg
     6841 ggaggcacga tcgccggcgg aagcgcgcgg gcgccgccgg gaaaggccgc cgggaacgtt
     6901 acgggccgca cgaccgagac gccggcaccg tccacgtccg cctcgaccac ggcccgcatc
     6961 gccg
//
LOCUS       DATLFO010000305         5855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7312, whole genome shotgun sequence.
ACCESSION   DATLFO010000305 DATLFO010000000
VERSION     DATLFO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7312"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="rplC"
                     /locus_tag="VM841_12355"
     CDS             complement(<1..433)
                     /gene="rplC"
                     /locus_tag="VM841_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HVL91016.1"
                     /translation="MSVVKGVLGRKLGMTQIFTEDGRAVPVTVVEAGPCAITQIRTPD
                     KDGYKAVQLSFGEVKAKQLNKPELGHLAKSGAAPTRHLVELRTDDIESYELGQVLGPD
                     VFAAGDKIDVIGLTKGKGFAGAMKRWNFAGKPRTHGTERKHR"
     gene            complement(430..750)
                     /gene="rpsJ"
                     /locus_tag="VM841_12360"
     CDS             complement(430..750)
                     /gene="rpsJ"
                     /locus_tag="VM841_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HVL91017.1"
                     /translation="MAQQQKIRIRLKAYDHEVVDQSARKIVDTVTRTGAKVSGPVPLP
                     TERSIYCVIRSPHKDKDSREHFEMRTHKRLIDILDPTAKTVDSLMRLDLPAGVDIEIK
                     AGGA"
     gene            complement(848..2032)
                     /gene="tuf"
                     /locus_tag="VM841_12365"
     CDS             complement(848..2032)
                     /gene="tuf"
                     /locus_tag="VM841_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HVL91018.1"
                     /translation="MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLSKSGKADF
                     TPFDQIDKAPEERERGITIAIAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVGVPYIVVALNKVDMVDDPELLELVELEVRELLNS
                     YEFPGDDVPVVRVSALKALEGDEQYEQGIIDLMAACDSAIPEPERDIDKPFIMAIEDV
                     FSITGRGTVVPGRIERGKVVVGEEIEIVGLRDTKKTTVTGVEMFRKLLDEGLAGDNVG
                     CLLRGIGKEDVERGQVLSKPGTITPHTHFEAQVYVLSKDEGGRHTPFFNNYRPQFYFR
                     TTDVTGSIVLPDGVEMVMPGDNTTMTVELIAPIAMDEGLRFAIREGGRTVGAGRVTKI
                     IK"
     gene            complement(2094..4223)
                     /gene="fusA"
                     /locus_tag="VM841_12370"
     CDS             complement(2094..4223)
                     /gene="fusA"
                     /locus_tag="VM841_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVL91019.1"
                     /translation="MAETQAAKTANKSGLVLREYPLGRTRNIGIMAHIDAGKTTTTER
                     ILYYTGRTYKVGEVHEGTAVMDWMVQEQERGITITSAATTCMWKDHWINIIDTPGHVD
                     FTVEVERSLRVLDGAVAVFDAVAGVEPQTETVWRQANRYQVPRICFVNKMDRIGADFY
                     RTVDMIKERLETVPLILHLPWGAEDQFHGVVDVVQMVAHFWPSDQLGEEWEDRPIPDE
                     LKAEAEKYHHDLIEALADFDEGVMEAFIGGTEPTVEELRQAIRRATLTGHASPVLCGT
                     AFKNKGVQPLLDAIIQFLPAPNDIPPVAGHVPGDGTATREPSDEQSFAALAFKIATDP
                     YVGKLTYFRVYSGQVRAGSHILNATKDKKERVGRILQMHANHREDREAAFSGDIVACI
                     GLKNTTTGDTLCDPSDPIVLESMTFPAPVISVAVEPKTKADQDKLSKALMALAEEDPT
                     FQVRFDDETSQTIISGMGELHLDILVDRLTREFKVGANVGKPQVAYRETVREAVKDVE
                     IRYVRQSGGRGQFAVVAIDLEPTGPGGGFEFVSKIKGGVLPNEYIPAVDEGVQEAMDS
                     GVLAGYPLVDVRVTLVDGQFHDVDSSEMAFKIAGSMALKEAARKAKPVLLEPVMEFEV
                     LTPSDFMGDVMGDVTSRRGAIDKIDQRGMMQSIRVRVPLAELFGYATDLRSKTQGRAS
                     HTPMQLHSYQQVPQSIAEQIVTKIRGE"
     gene            complement(4257..4727)
                     /gene="rpsG"
                     /locus_tag="VM841_12375"
     CDS             complement(4257..4727)
                     /gene="rpsG"
                     /locus_tag="VM841_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HVL91020.1"
                     /translation="MPRKGPVSKREIMPDPVYGSVLVTQLINKILTAGKRGTAEHIVY
                     KALDTVKEKSNTDQVSALKKALDNIKPTLEVRSRRVGGATYQVPVEVSARRGTTLALR
                     HLVQYSRDRKEKSMAERLAGELLDALNNQGAAVKRREDMHKMAESNKAFAHYRW"
     gene            complement(4731..5102)
                     /gene="rpsL"
                     /locus_tag="VM841_12380"
     CDS             complement(4731..5102)
                     /gene="rpsL"
                     /locus_tag="VM841_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HVL91021.1"
                     /translation="MPTIQQLVRKGRSPKRYKVRTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTTGMEVTAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYKIVRAAL
                     DTAGVKDRKQARSKYGAKRSG"
     gene            5437..5676
                     /locus_tag="VM841_12385"
     CDS             5437..5676
                     /locus_tag="VM841_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HVL91022.1"
                     /translation="MRTTLDIEDSLLRDAKVFAAESGRTLRSIVEEGLRRILEARTAP
                     TDFEPLPTLGGGAVREGVDLTNNAQVLDILDGFDE"
     gene            5673..>5855
                     /locus_tag="VM841_12390"
     CDS             5673..>5855
                     /locus_tag="VM841_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HVL91023.1"
                     /translation="MIILDVNVLVGAYRSEISYHQAMKGWLTNALAGPEPIGLAGVAV
                     AGFARIVTSRRLFSLPA"
BASE COUNT          883 a         1921 c         1907 g         1144 t
ORIGIN      
        1 agcggtgctt gcgctcggtg ccgtgcgtgc gcggcttgcc tgcgaagttc caacgcttca
       61 tcgcaccggc gaaacccttg cccttggtca agccgatcac gtcgatcttg tcgccggccg
      121 cgaacacgtc cggtcccagc acctgcccga gctcgtagga ctcgatgtcg tcggtgcgca
      181 gctcgaccag atgacgcgtc ggcgcggccc ccgacttggc caggtgaccc agctccggct
      241 tgttgagctg cttcgccttg acctcgccga acgacagctg aacggccttg tagccgtcct
      301 tgtccggcgt gcggatctgc gtgatcgcgc acgggcccgc ctcgacgacg gtcaccggaa
      361 cggcgcggcc gtcctcggtg aagatctggg tcatgcccag cttccgtccc agcacaccct
      421 tcacgacgct catgcgccac cggccttgat ctcgatgtcc acaccggcgg gcaggtcgag
      481 gcgcatcaac gagtcgaccg tcttggccgt cggatcgagg atgtcgatca gccgcttgtg
      541 cgtgcgcatc tcgaagtgct cgcgcgagtc cttgtccttg tgcggcgagc ggatgacgca
      601 atagatgctc cgctcggtgg gcagcggaac cggacccgag accttcgcac ccgtgcgcgt
      661 caccgtgtcc acgatcttgc gcgcggactg gtcgacgacc tcgtggtcgt aggccttgag
      721 cctgatccga atcttctgct gctgcgccaa cgtgctttct cctgtcttat tggggggcta
      781 tccatgagcc ccccaaaccc ctcctgcccg tggtcctcgt tgccgaggcc gcggccaaga
      841 gaatcgatta cttgatgatc ttcgtgactc gaccggcgcc gacggtgcgt ccaccctcgc
      901 gaattgcgaa gcggaggcct tcgtccatgg cgatcggcgc gatgagctcg acggtcatcg
      961 tcgtgttgtc gcccggcatg accatctcga cgccgtcggg cagaacgatc gagcccgtga
     1021 cgtcggtcgt gcggaagtag aactgcgggc ggtagttgtt gaagaacggc gtgtgacggc
     1081 cgccctcgtc cttggacagg acgtagacct gcgcctcgaa gtgcgtgtgc ggcgtgatcg
     1141 tgcccggctt cgacagaacc tggccgcgct cgacgtcctc cttgccgata ccacgcagaa
     1201 ggcagccgac gttgtcgccc gcaaggcctt cgtcgagcag cttgcggaac atctcgacgc
     1261 cggtgacggt cgtcttcttg gtgtcgcgca gaccgacgat ctcgatttcc tcaccgacca
     1321 cgaccttgcc gcgctcgata cgacccggga cgacggtgcc acggccggtg atcgagaaca
     1381 cgtcttcgat cgccatgatg aacggcttgt cgatgtcgcg ctccggctcc gggatcgccg
     1441 agtcgcatgc ggccatcaga tcgatgatgc cctgctcgta ctgctcgtca ccctcgagcg
     1501 ccttcagcgc cgacacgcgc acgaccggca cgtcgtcgcc cgggaactcg tagctgttga
     1561 gcagctcgcg aacctcgagc tcgacgagct cgagcagctc ggggtcgtcg accatgtcga
     1621 ccttgttcag cgcgacgacg atgtagggga cgccgacctg gcgggcgaga agcacgtgct
     1681 cgcgcgtctg cggcatcggg ccgtcggcgg ccgacacgac caggatcgcg ccgtccatct
     1741 gcgcggcacc cgtgatcatg ttcttgacgt agtcggcgtg gccggggcag tcgacgtgcg
     1801 cgtagtggcg gttgtcggtc tcgtactcga cgtgggcgat ggcgatggtg atgccgcgct
     1861 cacgctcttc cggcgccttg tcgatctggt cgaacggcgt gaagtccgcc ttgcccgact
     1921 tcgacaggac cttcgtgatg gcagcagtca gcgtcgtctt gccatggtcg atgtgaccga
     1981 tggtgccgat gtttacgtgc ggcttcgtcc gctcgaactt cgccttggcc atgtctgtct
     2041 cctaccctcc tcgccgggtc tccggcgaac acccaaaccg ttgaaccgtt ctcttactct
     2101 ccgcgaatct tcgtgacgat ctgctccgcg atcgactgcg gaacctgctg gtacgaatgc
     2161 aactgcatcg gcgtgtgcga cgcgcgaccc tgcgtcttgg aacgcaggtc ggtcgcgtag
     2221 ccgaacagct cggccagcgg aacgcgtacg cgaatcgact gcatcattcc gcgctggtcg
     2281 atcttgtcga tagcgccgcg gcgcgacgtc acgtcgccca tcacgtcgcc catgaagtcg
     2341 ctcggcgtga gcacctcgaa ctccatgacc ggctcgagca gaaccggctt cgccttgcga
     2401 gccgcttcct tgagcgccat cgaaccggcg atcttgaacg ccatctcgga ggagtccacg
     2461 tcgtggaact gcccgtccac gagcgtcacg cgcacgtcca ccagcgggta gccggcgagc
     2521 acaccggaat ccatcgcttc ctggacgcct tcgtccacgg ccgggatgta ttcgttcggc
     2581 agcacgccgc ccttgatctt ggacacgaac tcgaagccgc cgccggggcc cgtgggctcg
     2641 aggtcgatcg ccacgacggc gaactgaccg cggccaccgg actgacgcac gtagcggatc
     2701 tcgacgtcct tgacggcctc gcgcacggtc tcgcggtagg cgacctgcgg cttgccgacg
     2761 ttcgcgccga ccttgaactc gcgcgtgaga cggtccacca ggatgtccag gtgaagctcg
     2821 cccatgcccg agatgatcgt ctgcgacgtc tcgtcgtcga agcggacctg gaacgtcggg
     2881 tcctcttcgg cgagcgccat gagcgccttc gacagcttgt cctggtcggc cttcgtcttg
     2941 ggctcgaccg cgaccgagat gaccggcgcg gggaacgtca tcgactcgag cacgatcggg
     3001 tccgacgggt cgcacagcgt gtcgcccgtc gtggtgttct tcagcccgat gcacgcgacg
     3061 atgtcgcccg agaacgccgc ttcgcggtcc tcgcggtggt tggcgtgcat ctgaaggatg
     3121 cggccgacgc gctctttctt gtccttggtc gcgttcagga tgtgcgatcc cgcgcggacc
     3181 tgacccgagt agacgcggaa gtaggtgagc ttgccgacgt aggggtcggt cgcgatcttg
     3241 aacgccagcg ccgcgaagga ctgctcgtcg ctcggctcgc gcgtcgcggt gccgtcgccc
     3301 ggaacgtgtc cagcgaccgg cggaatgtcg ttgggcgcgg gcaggaactg gatgatcgcg
     3361 tccagcagcg gctgcacgcc cttgttcttg aacgcggttc cgcacaggac cggcgaggcg
     3421 tgaccggtca gcgtcgctcg gcggatcgcc tggcgcagct cttccacggt cggctcggtt
     3481 ccgccgatga aggcctccat gacgccctcg tcgaagtcgg cgagcgcctc gatcaggtcg
     3541 tggtggtact tctcggcctc ggccttgagc tcgtcgggga tcgggcggtc ttcccattcc
     3601 tcgcccagct gatccgacgg ccagaagtgc gcgaccatct gcacgacgtc cacgacgccg
     3661 tggaactggt cctcggcgcc ccagggcaga tgcaggatca gcggcaccgt ctcgaggcgc
     3721 tccttgatca tgtccacggt gcggtagaag tccgcgccga tccggtccat cttgttgacg
     3781 aagcagatgc gcgggacctg atagcggttg gcctgacgcc agaccgtctc ggtctggggc
     3841 tcgacaccgg cgacggcatc gaagacggcg accgcgccgt cgagcacgcg cagcgaacgc
     3901 tcgacctcga ccgtaaagtc cacgtgtccg ggcgtgtcga tgatgttgat ccagtggtcc
     3961 ttccacatgc aggtcgtcgc ggcggaggtg atggtgatgc cgcgctcctg ctcctggacc
     4021 atccagtcca tgaccgccgt gccctcgtgc acctcgccga ccttgtaggt tcggccggtg
     4081 taataaagga tgcgctcggt cgtggtggtc ttgccggcgt cgatgtgcgc catgatgccg
     4141 atgttgcgcg tgcgaccgag cgggtactcg cgaagcacga gcccgctctt gttcgccgtc
     4201 tttgccgcct gtgtctcagc catgtgtgtt cgctgtcttc tggtcgggga agtgggttac
     4261 caacggtagt gcgcgaaggc cttgttggat tcggccatct tgtgcatgtc ttcgcgccgc
     4321 ttcaccgcgg cgccctggtt gttgagcgcg tccagcagtt cgccggccag gcgctcggcc
     4381 atcgactttt ccttgcggtc ccggctgtac tgcacgaggt ggcgcagcgc cagcgtggtg
     4441 ccgcggcggg cgctgacctc gaccggaacc tggtacgtgg caccaccgac gcggcgtgaa
     4501 cgcacttcca gggtcggctt gatgttgtcg agcgccttct tcagcgccga gacctggtcg
     4561 gtgttgctct tctccttcac cgtgtcgagc gccttgtaga cgatgtgctc ggcggtgccg
     4621 cgcttgccgg cggtcaggat cttgttgatc agctgcgtga cgagcacgct gccgtagacc
     4681 gggtccggca tgatctcgcg cttggaaact ggacctttgc ggggcatggg ttagccgctc
     4741 ctcttggcgc cgtacttgga acgagcctgc ttgcggtcct tcacgccggc ggtgtccagc
     4801 gccgcgcgca cgatcttgta gcggacaccc ggaaggtcct tcacacggcc gccacgcacg
     4861 agcacgatgg agtgctcctg caggttgtgg ccgacgccgg ggatgtaggc cgtgacctcc
     4921 atgccggtcg tcaggcggac acgcgcgacc ttacgaaggg ccgagttcgg cttcttcggg
     4981 gtcgtggtgt acacgcgcgt gcagacgccg cgccgctggg gcgaaccctt cagcgcaggc
     5041 gtgcgaacct tgtatcgctt gggcgaacgg cccttgcgca cgagctgctg gatcgtcggc
     5101 aaacctgtac tcctctatcc acgttcttcg ttccgggcac acgacaccta agggtctcgt
     5161 gggcttgtaa tggacacgcg cccgggcgta cagatacccc tccgcccttc agacgcgagg
     5221 gcgaagacta gcaacagagg gtgtggatcg tcaacgcgac accgccgatc tgaactcgat
     5281 ccggctgcct gccgatcagg ttccgcttcc tcgacgcggg agtcgctgcg atacgcccgg
     5341 acgatcgcat gctcaggttg tccgacgagt ggctcttccg tccccgcgtc caacgaaggc
     5401 tcgggcttcc cgcatatata tgctatggtt ttatgcatgc gtaccacgct ggacattgag
     5461 gattcgctgc tcagggatgc gaaggtgttt gcggccgaga gcggacgcac gctgaggtcg
     5521 attgtggagg aagggctccg gaggatcctg gaagcgcgca ccgcaccaac ggacttcgag
     5581 ccgctgccga ctctgggggg cggcgccgta cgcgagggcg tcgatctcac gaacaacgcc
     5641 caggttctgg acattctcga tgggttcgac gagtgatcat cctcgacgtc aacgttcttg
     5701 ttggcgcgta tcgaagcgag atctcctacc accaggcgat gaagggctgg ctcacgaacg
     5761 cgctggcggg ccccgagccg atcggcttgg cgggagtcgc ggtggcgggg ttcgcgagga
     5821 tcgtcaccag ccgccgattg ttttcgctgc ccgcc
//
LOCUS       DATLFO010000306         6404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_38361, whole genome shotgun sequence.
ACCESSION   DATLFO010000306 DATLFO010000000
VERSION     DATLFO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38361"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VM841_12395"
     CDS             complement(<1..594)
                     /locus_tag="VM841_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVL91024.1"
                     /translation="MPGTKSKVATRNAAQKRVLEWYDEHHRSFPWRETRDPYRTMVAE
                     VMLQQTQTGRVAPIYDRFLHRFPTLSRLAHAPSMEVIQAWRGLGYNRRAVDLHRAAQA
                     IEHDHAGVFPHEPAELRRLPGLGEYTAHAIACFAFDADVPVVDTNVRRVLSRVALGKN
                     AAEVEPSRSSALAAEWLPRGEAYRWNQALMDVGAMICR"
     gene            complement(684..2144)
                     /locus_tag="VM841_12400"
     CDS             complement(684..2144)
                     /locus_tag="VM841_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91025.1"
                     /translation="MGLMVFTFLGTATGVAGNATASAFGAQLKINTAVTSSSLVGPIP
                     QAAADRNKTLENASLVRIGETPAGGALIQEIGALIVESKVDLVAGTARASSDTAHVGL
                     LRTTPAGVAPGGVGGGLLGGLLGNNVVPGAAPYNRIDVDAIHGIVNLNCNTVTSLRNS
                     SEKHRLETLTKGSQLLGLSIPDLGVNVKVADTLNERLGPDHYAYTRVRIDLAALGLPA
                     LPPPLNVLNGNLVEVTIYEVKGTNLTTASLTNATVSTTVDMLRVRVLDRTTLGVEVDL
                     RVGHAEASLSNCGPPPTPPTTHTPTTDPPTISTFHVDASKIVESINGAAVVTQSGLAA
                     ARGHEVEYSLNVFNRSLPGACSITRVVDHLPANTTFSSSAGDLGVGVYDAVAHTVTWD
                     NLAIPGQIAKRQGLVLRINENAPGGTYVNRFTATGTCGTFTAVAPAINVAVPIQVLGT
                     QFRETPRTGVADTALMVLGGLMLVAAIGGTRLISRA"
     gene            complement(2485..4974)
                     /locus_tag="VM841_12405"
     CDS             complement(2485..4974)
                     /locus_tag="VM841_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HVL91026.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRQQVEEIIGQGQTPPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNKVRQQVIQLLSGYQGGKEPGAPQGAPGE
                     QAPPGSLVLDQFGRNLTQLARDGKLDPVIGREKEVERVMQVLSRRTKNNPVLIGEPGV
                     GKTAIVEGLAQQIVGGEVPETLKGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIRT
                     RGDIILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLEEYRKHLEKDA
                     ALERRFQPIQVAEPSLPHTIEILKGLKDRYEAHHRVTITDEAVVAAANLADRYISDRF
                     LPDKAIDLIDEAGSRMRIRRMTAPPDLREFDEKIADVRREKESAIDSQDFEKAASLRD
                     DEKNLMADRAVREKEWKAGEMDVTAILGEEEIAEVLAIWTGIPVYKLTEEETAKLIRM
                     EDELHKRIVNQSEAVKSVSQAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELAKTLAE
                     FLFGDESSLIQLDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRRPFSVVLFDE
                     IEKAHPDVFNTLLQILEDGRLTDAQGHTVDFKNTVIIMTSNLGTRDLDKARAVGFSTK
                     VDEGDTYQRMQEKVHEELKRSFRPEFINRIDEIIVFHPLTQQHVKEIVDLMMKRVHDQ
                     LKAKDIEIVLTEAAKDHLAVKGYDQALGARPLRRTIQRLIEDPLSEKLLWKEFSAAQT
                     VIVDEQDGELTFESAPSPPNTPPIELAERGS"
     gene            complement(5810..>6404)
                     /locus_tag="VM841_12410"
     CDS             complement(5810..>6404)
                     /locus_tag="VM841_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HVL91027.1"
                     /translation="QPRTTSSSREYGWLGTKQRPTDPNSGIILMGVRLYTPTAGRFLQ
                     IDPVKGGNANDYDYCTADPINCYDLDGRLGWLRKAANFVWKHKWDIALTAASFTGVGA
                     AAWTYRAYRVVRVARTTRGMAGGIRATRATSWLAGRMWTGIGSRSTAVGRVSSNRLRQ
                     YRNPAFKSRLGIAQSNFESRAATSGRWSNNYHVRIWP"
BASE COUNT         1023 a         2065 c         2095 g         1221 t
ORIGIN      
        1 gcggcagatc atcgcgccga cgtccatcag agcctggttc catcggtacg cctcgccacg
       61 cggcagccat tccgccgcca gagccgagga gcgggatggc tcgacttctg cggcattttt
      121 gccgagcgcc acccgcgaca acacccggcg cacgttggtg tccaccaccg gcacgtcggc
      181 atcgaaggcg aagcatgcga tcgcgtgcgc ggtgtattcg cctagtcccg gcaaacgtcg
      241 tagctcggcg ggctcgtggg gaaacacgcc ggcgtggtcg tgctcgatcg cctgcgcggc
      301 gcggtgcagg tccacggcgc ggcggttgta tcccaggccg cgccaggcct ggatgacctc
      361 catcgacggc gcgtgcgcaa gccgggacag ggtcgggaaa cgatgcagga agcggtcgta
      421 gatcggagcc acccggccgg tctgcgtctg ttgcagcatg acctcggcga ccatcgtgcg
      481 gtacgggtcg cgcgtttcgc gccacgggaa cgagcggtga tgctcgtcgt accactccag
      541 cacgcgcttc tgcgcggcgt tgcgcgtggc caccttgctc ttggttcccg gcatcgattt
      601 cagtctacga ctgatggccg attccggcgt tcggacgagg aagaggggca cccgtcgggt
      661 gcccctcgcc cttttcgata cggctatgcg cggctgatca ggcgcgtgcc gccgatggcg
      721 gcgacgagca tcaggccgcc gagcaccatc agcgcggtgt cggcgacgcc ggtgcgcggc
      781 gtttcacgga actgcgtgcc gagcacctgg atcggcactg cgacgttgat cgccggcgcg
      841 accgcggtga acgttccgca ggttccggtt gcggtgaagc ggttcacgta ggtgccgccc
      901 ggcgcgttct cgttgatacg caggaccaga ccctgacgtt tggcgatctg gccggggatc
      961 gcgaggttgt cccaggtgac cgtgtgcgcg acggcgtcgt agactccgac accgaggtca
     1021 ccggcgctcg acgagaacgt ggtgttcgcc gggagatggt ccacgacccg ggtgatcgag
     1081 caggcgcccg ggagcgaccg gttgaacacg ttcagcgagt actcgacctc gtgtccccgc
     1141 gcggcggcca ggccgctctg ggtcacgacc gcggcgccgt tgatcgactc gacgatcttg
     1201 gaggcgtcca cgtggaaggt cgagatcgtc ggaggatcgg tcgtcggcgt gtgcgtcgtc
     1261 ggaggagtcg gcggcggccc gcagttcgac agactcgcct cggcgtggcc gacgcgcagg
     1321 tccacctcga cgccgagcgt cgtgcggtcg agcacgcgga cgcggagcat gtccaccgtc
     1381 gtgctcaccg tcgcgttcgt cagcgacgcc gtggtcaggt tggtgccctt cacctcgtag
     1441 attgtcacct cgacgaggtt gccgttcagc acgttcagcg gcggaggcag cgcgggcaga
     1501 ccgagggccg ccaggtcgat gcgcacccgc gtgtacgcgt agtgatcagg accgagccgc
     1561 tcgttgagag tgtcggccac cttgacgttc acgcccaggt ccgggatgga aaggccgagc
     1621 agctgcgatc ccttggtgag cgtctcgaga cggtgcttct cgctcgagtt gcgcagcgag
     1681 gtgacggtgt tgcagttgag gttcacgatg ccgtgaatcg cgtccacgtc gatgcggttg
     1741 tacggagcag cgcccggcac cacgttgttt ccgaggagcc cgccgaggag cccgccgccc
     1801 actccgcccg gcgcgacgcc ggccggagtt gtgcgaagca gcccgacgtg cgcggtgtcc
     1861 gaggaggcgc gtgccgtgcc ggccaccaga tcgaccttgg actcgacgat gagcgcgccg
     1921 atctcctgga tgagcgcgcc gcccgccggc gtttcgccga tgcgaacgag cgaggcgttc
     1981 tccaacgtct tgttgcggtc ggccgcagcc tgcgggatcg gacccacgag cgaactcgag
     2041 gtcacggccg tattgatctt gagctgagcc ccgaacgcgg aggccgtcgc gttgcccgcg
     2101 acgcccgtgg ccgttccgag gaatgtgaaa accatcaacc ccaccgcggc caaagctatg
     2161 accgcgaggg agctcaaacc gaacgcttta cgcgccataa agccgcaccc ccacttccgt
     2221 cgtactcgtc gtcattcgtg gtgttcgttc ccattgtgta ccccgagtga gcaattgtat
     2281 atccccaact gtcccaaaat ggtacaaagc ggcagcgaaa cttgcactat ccgtccgcac
     2341 agagcggcga atcaccccgc aacctagcga aacaacccaa ggaatgacca atcgggctcg
     2401 acgggagaat ggatcgctgc ggttaaagcg tgcgaaagca agacccccgg ttgatcaccg
     2461 ggggtctcga tgcttcgggc cggatcagga gccgcgttcg gccagctcga tcggcggcgt
     2521 gttcggcggg gacggagccg actcgaaggt cagctcgccg tcctgctcgt ccacgatcac
     2581 cgtctgagcg gccgagaact ccttccacag cagcttctcg gacagcgggt cctcgatcag
     2641 acgctggatg gtgcggcgca acggccgggc gcccagcgcc tggtcgtagc ccttgaccgc
     2701 caagtggtcc ttcgcggcct cggtcagcac gatctcgatg tccttcgcct tgagctggtc
     2761 gtgcacgcgc ttcatcatca ggtccacgat ctccttgacg tgctgctgcg tcagcgggtg
     2821 gaagacgatg atctcgtcga tccggttgat gaactcgggc cggaacgagc gcttcagctc
     2881 ctcgtggacc ttctcctgca tgcgctgata cgtgtcgccc tcatcgacct tggtcgagaa
     2941 gccgaccgcg cgtgccttgt ccagatcccg cgtccccagg ttcgaggtca tgatgatcac
     3001 ggtgttcttg aagtccaccg tgtgaccctg cgcgtcggtc aggcgtccgt cttcgagaat
     3061 ctgaagcagc gtgttgaaca cgtcggggtg cgctttctcg atctcgtcga acagcaccac
     3121 gctgaacggc cggcggcgaa ccgcctcggt cagctggccg ccctcttcgt agccgacgta
     3181 gcccggcggc gaaccgatca ggcgcgacac cgtgtgcttc tccatgtatt cgctcatgtc
     3241 gagctggatc agcgaggact cgtcaccgaa caggaattcg gcgagcgtct tggccagctc
     3301 ggtctttccg acgcccgacg ggcccaagaa gatgaacgag ccgctgggac gcttcggatc
     3361 cttcaacccg gcgcgcgtcc ggcgaatcgc ctggctgacg gacttgacgg cctcggactg
     3421 gttgacgatg cgcttgtgca gctcgtcttc catgcggatc agcttggcgg tctcttcctc
     3481 ggtgagcttg tagaccggga tgcccgtcca gatggccagg acctccgcga tctcctcttc
     3541 gccgaggatc gcggtgacgt ccatctcgcc ggccttccat tccttttcgc ggacggcgcg
     3601 gtcggccatc aggttcttct cgtcgtcgcg caggcttgcg gccttctcga agtcctgact
     3661 gtcgatcgcg gactccttct cgcggcgaac gtcggcgatc ttctcgtcga actcgcgaag
     3721 atccggcggc gcggtcatgc gacggatgcg catgcgcgag ccggcctcgt cgatcaggtc
     3781 gatcgccttg tcgggcagga agcggtcgct gatgtagcgg tcggcaaggt tggcggccgc
     3841 gacgacggct tcgtcggtga tcgtgacgcg gtggtgcgcc tcgtagcggt ccttcaggcc
     3901 cttgaggatc tcgatggtgt gcggcagcga cggctcggcg acctggatcg gctggaagcg
     3961 ccgctccaat gctgcgtcct tctcgaggtg cttgcggtac tcctcgagcg tggtcgcgcc
     4021 gatcgtctgc agctcgccac gggccagcat cggcttcaag atgctcgcgg cgtcgatcgc
     4081 gccttcggcg gcacccgcgc cgacgagcgt gtgcagctcg tcgatgaaca ggatgatgtc
     4141 gccgcgcgtg cggatctctt tcagaacctt cttcagacgc tcttcgaagt cgccgcggta
     4201 acgagagccg gcaaccagcg cgcccagatc cagcgtgtag atctgcttgc ccttgagcgt
     4261 ctcggggacc tcgccgccga cgatctgctg ggcgaggcct tcgacgatcg ccgtcttgcc
     4321 gacgccgggc tcgccgatca gaaccgggtt gttcttggtg cggcgcgaca ggacctgcat
     4381 gacgcgctcg acttctttct cgcggccgat gaccgggtcc agcttgccgt cgcgggcgag
     4441 ctgggtcagg ttgcggccga actggtccag caccagcgaa ccgggcgggg cctgctcgcc
     4501 gggcgcgccc tgcggagcgc cgggctcttt gccgccctgg tagccggaca gaagctggat
     4561 gacctgctgg cggaccttgt tcaggtcggc gcccagcttg accaggacct gcgcggcgac
     4621 gccttcgcct tcgcggatca gaccgagcag gatgtgctcg gtgccgatgt agttgtggcc
     4681 cagctgcaac gcttcgcgca gcgacagctc cagaaccttc ttggcgcggg gcgtgaacgg
     4741 gatgtggccg gtcggaggcg tctgaccttg accgatgatc tcctcgacct gctggcggac
     4801 ggcctcgagc gagattccga gcgattcgag cgccttggcg gcgacccctt cgccctcgtg
     4861 aatcaagccg aggaggatgt gctcggtacc gatgtagttg tggttgagca tccgcgcttc
     4921 ttcctgcgcg aggacgacaa cccggcgggc tcgatccgta aaccgctcaa acacgatgtc
     4981 agccttcctc ttctttacgt caggcccgga ttatatctgc cggccctgcc tcttcccccg
     5041 gcttatccca cgccctcggg aagacgcaac gccgcctttc acgtcctgat tctcaccatc
     5101 tgtgatgcct aaacgacgga ccttcatatt tcatacgagt cttcggcgct cgcggaccgc
     5161 ttcctgagaa acaaaagccg gccgcggggc cggtcccgga tctctttcga tgagatgaac
     5221 agcgtcccgg tgaccgctcc tattcccact atcggcctca ggatgggaac gtcacaatga
     5281 ccagaacgga cggtttcatc cctagtccgc tcacgcagac aggtcgtcgg aaacgctcag
     5341 tgaccgccgg ctagaagcgg actggcctgt actgcctcgc cgagcagacc cagcatcgcg
     5401 gaaggtcgga gccggttcgc ttcagggagc tggttcccct agagatgctt aggctccgcc
     5461 gcgctcgcgt cctgaaacaa gttgcgccgc gacagtgatc ttcggatgcc gagataaagg
     5521 gcggcggggc cgacgaaaat caagtagcac actgaatcgc accgggattc ctggaggcat
     5581 tggtcttccc ccacttaagg tagccattca acgtccagcc ggttgatcca tgctcgcagt
     5641 tgcatatcgt catcagccca gatgtcccac ccgtccgacg acccgcgacg aggatccgag
     5701 cgtaggatcc aaatgtgcca gcctgtttct gaaggatccc gttggacctc gacgaagaag
     5761 cccttgtatt ctacggcccg tgcccgaccc gtgcgcccga tgtaatcgtt cacggccaga
     5821 ttctcacgtg gtagttgttg gaccaacgac cgcttgtggc cgcgcggctc tcgaagttcg
     5881 actgcgctat gccaagtctc gatttgaacg ccggattccg gtattgacga agcctgttgg
     5941 agctgacgcg gcctacagcc gtcgatcgtg acccgattcc ggtccacatc cggccggcca
     6001 gccaactcgt tgcgcgggta gcgcgtatcc ctccagccat tcctcgagtt gttctggcga
     6061 cacgcacgac gcgataagct ctgtaggtcc aggccgccgc tccaacgccg gtaaaagacg
     6121 cggccgtcag cgcgatgtcc cacttgtgtt tccagacgaa gtttgcggcc ttcctaagcc
     6181 atcccaggcg tccgtcgagg tcgtagcagt tgatggggtc ggcggtgcag tagtcgtagt
     6241 cgttggcgtt gccgcccttt actgggtcga tctggaggaa tcgtccggcg gtgggtgtgt
     6301 agaggcggac gcccatgagg atgatgccgc tgttggggtc ggtggggcgt tgtttggtgc
     6361 cgagccatcc gtattcgcgg ctgctgctgg tggtgcgggg ttgg
//
LOCUS       DATLFO010000307         4278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_126006, whole genome shotgun sequence.
ACCESSION   DATLFO010000307 DATLFO010000000
VERSION     DATLFO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126006"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /gene="mrdA"
                     /locus_tag="VM841_12415"
     CDS             complement(<1..1698)
                     /gene="mrdA"
                     /locus_tag="VM841_12415"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVL91028.1"
                     /translation="MNRAYGDDDRIRVRVSALGLIMLALISVLGSRLWFLQVLANDQY
                     AAAATANYVRLVSVPAPRGKILDRNGKVLVGTRSSLTVGVRRTDLRDKDAVLNRLADL
                     VGITREVLDKRLADKRVSPYKPVTVARDVPQETWLYLREHAAEFPGVETDVEPVRLYP
                     EGGVAAHVIGYVGEINEKELQERRDQGYRLGDEIGRAGVERSYEHILRGKPGLEKIEV
                     DSRGTPLRTLGSEPPVPGKDLQLTLDLEVQKVAEQSLFEGIQRARGRVFEETKQRFVA
                     PAGGVVVLDAKTGEVVAMASYPTFDLKKFSGGIDPEYWRFLNDPVNEQPLLNRVIQSA
                     YPPGSTFKPFVAAGALMTGQATPTARFACTTEFEYGGRIFRNWRPRNSTITLAQALIE
                     SCDTPFYRIGANWWAKERERERANNSVYEAMQDWAVKFGLGRPTNIDFGTETKGRMPG
                     RAYKRDQWRKNRDAWCERFRATKEQAWEDLCVRGYVWRGGDSVNMSIGQGDLAVTPLQ
                     LALAYGALANGGNVMRAYVGKQAVDPETKDSSGLIEPAVMQTAGLEARYHDYIRDALV
                     "
     gene            complement(1695..2207)
                     /gene="mreD"
                     /locus_tag="VM841_12420"
     CDS             complement(1695..2207)
                     /gene="mreD"
                     /locus_tag="VM841_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HVL91029.1"
                     /translation="MIWRRSLLLGMLMLTGLLLETTLLGGLTLGGTQPQLVLLIVIAL
                     GMAEGPATGAVAGFVGGVLTDMLLSLPTGLSALVFTSIGYSVGALREHYEGTTALLTI
                     VVAGLATATGVIGYGAVSVLFGRVGLVPADVLRHAGLAAVYSMLLAPFVLPGIRRVSE
                     RLRPRKVVRL"
     gene            complement(2204..3181)
                     /gene="mreC"
                     /locus_tag="VM841_12425"
     CDS             complement(2204..3181)
                     /gene="mreC"
                     /locus_tag="VM841_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HVL91030.1"
                     /translation="MLRLNERTRRVRLLLVVLLLASLTLVTIDFRSDGNGPLTRIGNA
                     VASVFGPLQDSLSSVTRPIGNFFSGFGQVGSLKAQVRSLQEELALLRQDQGRVLDVLA
                     ENERLRSLIEIKDRLGLKTKAATVIGRDSSNFQQSIVIDIGTKDGVKKDMPVVGGEGL
                     VGRVVQASASTATVLLIVDPSSDVASRVAETGEVGVLSGGGRRDMRLTLLNPNAIVTA
                     GDSVVTASYRPTKAGSSVFPDGIPIGTISRVDPPGGNVTRRAFVKPAVDFTTLDFVLV
                     VTGIGTPRPLPVPPVSPSPSPSPQGSVPASPSPHSSPRPSPHPSPGASR"
     gene            complement(3286..>4278)
                     /locus_tag="VM841_12430"
     CDS             complement(3286..>4278)
                     /locus_tag="VM841_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVL91031.1"
                     /translation="IDLGTANTLVYVRGRGIVLNEPSVVAINSKTGAILAVGAEAKRM
                     MGRTPSHITVVRPLKDGVIADFEVTEKMLRYFIQKVHRRRWLAKPRVVICVPSGITGV
                     EQRAVEEATVRAGARSAFIIEEPMAAAIGAGLPIHEPVGNVVVDIGGGTTEVAVVSLG
                     GFVTSASIRIGGDELDEAIVNGIKKEYSLLVGLQTAEEIKKTIGSAFPLADEPHAEIR
                     GRDLVTGLPKTIIVSAEEIRRAIEEPVNQIVDAVKNTLEKTPQDLADDIIDKGIVLTG
                     GGALLKGLDERLRHETGMPIHIAEGALQCVAIGSGKCLEEFEALKRVLISTSRH"
BASE COUNT          674 a         1482 c         1445 g          677 t
ORIGIN      
        1 caccaacgcg tcgcgaatgt agtcgtggta gcgggcctcc aggccggccg tctgcatgac
       61 ggcgggctcg atcagcccgc tggagtcctt ggtctcgggg tcgacggcct gcttgcccac
      121 gtaggcgcgc atcacgttcc caccgttggc gagcgcgccg taggcgagcg ccagctgcag
      181 cggcgtgacg gccaggtcgc cctgcccgat cgacatgttg accgagtcgc cgcctcgcca
      241 cacgtagccg cgcacgcaca ggtcctccca cgcctgctcc ttggtcgcgc ggaagcgctc
      301 gcaccaggcg tcgcgattct ttcgccactg atcccgcttg taggcgcggc cgggcatgcg
      361 gcccttcgtc tcggttccga agtcgatgtt cgtgggacgg ccgagaccga acttcaccgc
      421 ccagtcctgc atggcctcgt agacggagtt gttggcgcgc tctcgctcgc gctctttcgc
      481 ccaccagttc gccccgatgc ggtagaaggg cgtgtcgcag gactcgatga gcgcctgcgc
      541 gagcgtgatc gtggagttcc gcggccgcca gttgcggaag atgcggccgc cgtactcgaa
      601 ctcggtcgtg catgcgaagc gcgccgtggg cgtcgcctga ccggtcatga gcgcaccggc
      661 cgccacgaac ggcttgaacg tcgagccggg cggataggcc gactggatca cgcggttgag
      721 cagcggctgc tcgttgacgg gatcgttgag gaacctccaa tactcgggat cgatgccgcc
      781 ggagaacttc ttgaggtcga aggtgggata cgaagccatc gccacgacct cgccggtctt
      841 ggcgtcgagc accaccacgc cgccggccgg cgcgacgaag cgctgcttgg tctcttcgaa
      901 cacgcgcccg cgcgcacgct ggattccttc gaacagcgac tgctcggcga ccttctgcac
      961 ctccaggtcg agcgtgagct gaaggtcctt gccgggaacc ggcggctcgg agccgagcgt
     1021 gcgcagtggc gtgccgcgcg agtccacctc gatcttctcc aagcccggct tgccgcgcag
     1081 gatgtgctcg tagcttcgct cgacgccggc gcggccgatc tcgtcgccga ggcggtatcc
     1141 ctggtcgcgg cgctcctgaa gctccttttc gttgatctcg ccgacgtagc cgatcacgtg
     1201 cgcggcgacg ccaccttcgg gatacagccg gaccggctcg acgtcggtct cgacgccggg
     1261 gaactcggcg gcgtgctcgc ggaggtagag ccaggtctcc tgcggcacgt cgcgcgcgac
     1321 ggtgaccggc ttgtagggcg agacgcgctt gtccgccagc cgcttgtcga gcacctcgcg
     1381 cgtgatgccg accagatcgg cgaggcggtt gagcaccgcg tccttgtcgc gcaggtcggt
     1441 gcggcgcacg cccacggtca gcgacgagcg cgtaccgacc aggaccttgc cgttgcggtc
     1501 caggatcttg ccgcgcggcg ccggtaccga cacgagccgg acgtagttgg cggtcgcggc
     1561 cgccgcgtac tggtcgttgg cgagcacctg caggaaccac agccgcgagc ccaggaccga
     1621 gatcagcgcg agcatgatca acccgagcgc cgacacgcgc acgcggatcc ggtcgtcgtc
     1681 gccgtaggcc cggttcacag ccgcaccacc ttgcgcgggc gcaaccgttc gctgacccgg
     1741 cgaatcccgg gcaacacgaa cggggcgagc agcatgctgt agacggccgc aagtcccgca
     1801 tgcctgagca cgtcggcggg gaccaggcct acgcggccga acaggacgct gacggcgccg
     1861 tagccgatca cgccggtcgc ggtcgccagg ccggccacca cgatggtcag caaggccgtg
     1921 gttccctcgt agtgctcgcg aagggcgccg acggagtatc cgatcgaggt gaaaaccagc
     1981 gcgctcaatc ccgtgggaag gctcagcagc atgtcggtga ggaccccgcc gacgaacccg
     2041 gcgaccgcgc cggtggcggg cccctcggcc atgccgagcg cgatcacgat gagcaggacg
     2101 agctgcggct gggtcccgcc gagcgtgagc ccgcccagca aggtggtctc gagcagcagc
     2161 ccggtgagca tcagcatgcc gagcagcagc gagcggcgcc agatcatcgc gacgctcccg
     2221 gggagggatg gggggacgga cggggagacg aatgcggcga cggcgaggcc ggaaccgagc
     2281 cctgcgggga gggcgagggc gagggcgaca ccggcgggac cggcagggga cgcggcgtcc
     2341 cgatcccggt cacgaccaag acgaagtcca gcgtcgtgaa gtccacggcg ggcttgacga
     2401 acgcacggcg ggtgacgttg ccgcccggcg ggtcgacgcg cgagatcgtt ccgatcggga
     2461 tcccgtcggg aaagacgctg ctcccggcct tggtcggccg gtacgaggcg gtcacgaccg
     2521 agtcaccggc cgtgacgatg gcgttggggt tgagcaaggt cagccgcatg tcgcgacggc
     2581 cgccgcccga cagcacgccg acctcgccgg tctcggccac ccgggaagcc acgtccgagg
     2641 acggatcaac gatgagcagc acggtcgcgg tcgaggcgga cgcctggacc acccgtccga
     2701 ccaaaccctc gccgccgacg accggcatgt ccttcttgac gccgtccttg gttccgatgt
     2761 cgatgacgat cgactgctgg aagttcgagg aatcccgccc gatcacggtc gcggccttcg
     2821 tcttgagacc gagccggtcc ttgatttcga tcagcgaccg aagccgctca ttctcggcga
     2881 gcacgtcgag cacccggcct tgatcctgcc gcagcagcgc gagctcttcc tggagcgagc
     2941 ggacctgggc cttgagcgag ccgacctgac cgaagccgga gaagaagttc ccaatgggcc
     3001 gcgtcaccga cgagaggctg tcttgcagcg ggccgaacac ggatgcgacc gcattgccga
     3061 tccgggtcaa cggaccgttg ccgtcgctgc ggaagtcgat ggtgaccagc gtgagcgagg
     3121 ccagcagcaa aaccaccagc agcagccgca cccggcgggt gcgctcattg agcctgagca
     3181 tcacaccggg ccgcgccgcc gctcctcgag gcggcgcgag cccttcaatc ccctttcaac
     3241 cgggccgcgc cttcatcggc gcgatcccga acgcttattc gtccgtcagt gccgggacgt
     3301 ggagatcaag acgcgcttga gcgcctcgaa ctcctccaag cacttgcccg acccgatcgc
     3361 cacgcactgc agcgctcctt cggcgatgtg gatcggcatt ccggtctcgt ggcgcagccg
     3421 ctcgtccagt cccttcagca gcgcaccacc gccggtgagc acgattccct tgtcgatgat
     3481 gtcgtcggcc aagtcctgag gcgttttctc cagggtgttc ttgaccgcat ccacgatctg
     3541 gttgaccggt tcctcgatcg cgcggcgaat ctcctccgcc gacacgatga tcgtcttggg
     3601 cagtcccgtc accaagtcgc gcccacggat ctccgcgtgg ggctcgtcgg ccagcgggaa
     3661 cgccgatccg atggtcttct tgatctcttc cgccgtctgg aggccgacca gcagcgaata
     3721 ctccttcttg atgccgttga cgatcgcctc gtccagctcg tctccgccga tgcggatcga
     3781 ggcgctcgtc acgaatcctc ccagcgagac gaccgcgacc tcggtcgtgc cgccgccgat
     3841 gtccaccacc acgttgccga ccggctcgtg gatcgggagc cccgcgccga ttgccgccgc
     3901 catgggctcc tcgatgataa aggcgctccg ggctccggcg cggacggtgg cctcttcgac
     3961 cgctcgctgc tccacgccgg tgatgccgct cggcacgcag atcacgacgc ggggcttggc
     4021 cagccagcgc cggcggtgca ccttctggat gaagtaccga agcatcttct cggtcacttc
     4081 gaagtctgcg atgacgccgt ccttgagcgg ccggacgacg gtgatatggc tgggcgtacg
     4141 gcccatcatt cgcttggcct cggctcccac ggccaggatc gcaccggttt tggagttgat
     4201 cgcgaccacc gagggctcgt tgagcacgat cccccgtccg cggacgtaga cgagcgtgtt
     4261 cgcggtaccg aggtcgat
//
LOCUS       DATLFO010000308         4765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_77509, whole genome shotgun sequence.
ACCESSION   DATLFO010000308 DATLFO010000000
VERSION     DATLFO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77509"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VM841_12435"
     CDS             complement(<1..1053)
                     /locus_tag="VM841_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVL91032.1"
                     /translation="MTTGRLAAFPPGIKGEMEIREVEVPDPAPGEILIRVTRANVCGS
                     DLHFWRGDIDLSRFLKEPTVLGHEMCGRVAALGDGVATDSAGAPLAEDDRVVYRYFSP
                     CGRCSACLRGFTAACGRNAIFQIRVCTEAPYFVGGFGDYYMLPANQAVFKVPGDLDDD
                     LVSGVNCAVAEVMEGLTRGRVGPGDTVVIQGAGGLGLYATALARYMGATKVIVIDGVP
                     GRLSLAREFGADEVIDIGEHGDAGARSYRVRQLNDGNGADVAVEVAGFPDAFTEGIML
                     LGQGGRYVEIGNISMGMTTTFDPAQVTLGNKTVYGVAFYEAIALKRSLEFLAATKDRV
                     PYDRLISTPYPLEKIND"
     gene            complement(1050..1937)
                     /locus_tag="VM841_12440"
     CDS             complement(1050..1937)
                     /locus_tag="VM841_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91033.1"
                     /translation="MTVRRIRLLAATALLALLTAACQMTVDVTTAMGDDGRGTFRIVF
                     AFDKEFIEVVRSTEQGSATLDELAETGEQFGGTGWTVRQTRPQGGLRIDISRSFDDPG
                     ELERALREVERESGDRALSFPSVFRDFRIDHSSSFFRTSAEVTGSVDLRPERLVPGAD
                     AGEELRAALRQAARDVFRFRVSLDLPGRVGRYRGDPERVSGGSIVWTVPFGDRLEFAA
                     SSDGFKTSSLLIVLGPAAALLAGAAIVPVRRRRRERAIAVPGWDVPREETAPPADSAS
                     VPSAPRGDGDDDSTHGGSE"
     gene            complement(1965..2963)
                     /locus_tag="VM841_12445"
     CDS             complement(1965..2963)
                     /locus_tag="VM841_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL91034.1"
                     /translation="MKTGLALPHYDFSYPDGRSADLAATVEWACRAETLGYDSVWVSD
                     HFFLEITRYGGPPGRHGSLEPLAALGAIAAATERVRLGTLVLSEAFRPPPMLAKAAAT
                     LDVASGGRLEIGMGAGWYEDEYAAYGYPFGSAGERMQRLEEAVRIVGGLLDGGPVTFE
                     GEHYAVRDAINEPRPVQRPRPPIWIGSKGGPRAARIVAEAADGWNTVWKWTPEDYAAK
                     AATLDEACERAGRDPAGVRRSLGLYTLVGDDLPKLFEAVRDWAPGTMLEGTSLEEFAA
                     GALVGTAEQCAERMAAFAHLGADELIVAPAPLPFAIAVPEQVEAVAETLIPLLRDL"
     gene            3002..3787
                     /locus_tag="VM841_12450"
     CDS             3002..3787
                     /locus_tag="VM841_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HVL91035.1"
                     /translation="MDAPARFALAVNRPEPSVDTAEACFLIAANVYPELDVASELRRV
                     DALAVRCPPGFDGLRRYLFDELGFRGNTERYDDPRNSFLNDVLDRRVGLPITLSILAI
                     EVGRRVGVPLSGVGMPAHFVVRHDAEPGLFVDAFAGGETLDAAGCRDRFHEIVGQDVP
                     FDPDWLKPVSTTDILLRVLANLKNVYSQKTQWHHVEWVMRCRLSVPGCPAEERRDLAH
                     AIGQQGRFEEAARELDLLARITPDPDARHAITSEAITMRAQLN"
     gene            3904..4485
                     /locus_tag="VM841_12455"
     CDS             3904..4485
                     /locus_tag="VM841_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91036.1"
                     /translation="MRRRTFDTLMSLGGLAMTMVLLIAGSLLFVGYTYANNTVTSQLK
                     AQSIFFPAADNPQMQDPRIGPFVAPYAGEQVVTGRQAEVYANHFIGVHLEDVAEGKTY
                     SEVSTLSRANPDDATLAGQVQTLFRGETLRGVLLTAYAFWQLGQIALWGSIASFALAI
                     VMATLTVLGFWHRRRVAPEEEILAPPRGLAEAV"
     gene            complement(4544..>4765)
                     /locus_tag="VM841_12460"
     CDS             complement(4544..>4765)
                     /locus_tag="VM841_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HVL91037.1"
                     /translation="TDAYVGSMSEVRSSPETPTVLEDYVGLGDEVVLQPGTRISGNIT
                     IWPRLRIPAAAAIPPGVEIRDADDVMRYL"
BASE COUNT          714 a         1703 c         1653 g          695 t
ORIGIN      
        1 gtcgttgatc ttctccagcg gatacggcgt cgaaatcagc ctgtcgtagg gcacgcgatc
       61 tttggtcgcg gcgaggaact ccagcgagcg tttcagcgcg atcgcttcgt agaacgcgac
      121 cccgtagacg gtcttgttgc ccagcgtcac ctgcgccgga tcgaaggtgg tggtcatccc
      181 catcgagatg ttgccgatct cgacgtagcg gccgccttgg ccgagcagca tgatgccttc
      241 ggtgaacgcg tcggggaaac cggcgacttc gacggcgacg tcggcgccgt tgccgtcgtt
      301 gagctggcgc acgcggtagc tgcgcgcgcc cgcgtcgccg tgctcgccga tgtcgatgac
      361 ctcgtcggcg ccgaactcgc gagcgagcga cagccgcccg ggcacgccgt cgatcacgat
      421 gaccttggtc gcgcccatgt agcgcgccag cgccgtggcg tacaatccca gcccgcccgc
      481 gccttggatg accacggtgt cgccgggccc gacccggccg cgcgtcaggc cttccatcac
      541 ctcggcaacc gcgcagttca cgcccgagac cagatcgtcg tccaagtcgc ccggcacttt
      601 gaacaccgcc tgattcgcgg ggagcatgta gtagtcgccg aaaccaccga cgaaataggg
      661 cgcctcggta cagacgcgga tctggaagat cgcgttgcgg ccgcatgcgg cggtgaagcc
      721 gcgcaggcac gcgctgcacc ggccgcacgg gctgaagtag cgatacacga cccggtcgtc
      781 ttcggccagc ggcgcgcccg ccgagtcggt ggcaacgccg tcgcccagcg ccgcgacgcg
      841 gccgcacatc tcgtggccca gcaccgtcgg ctctttcagg aagcgcgaca ggtcgatgtc
      901 gccgcgccag aagtgaaggt ccgagccgca cacgttcgcg cgcgtgacgc gaatcaaaat
      961 ctcgcccggt gccggatcgg gcacctcgac ctcgcgaatc tccatctcgc ctttgatgcc
     1021 gggcgggaag gccgccagcc gtcctgtcgt cattcggatc ctccatgcgt cgagtcgtca
     1081 tcgccgtcgc cccgcggcgc cgacgggacc gatgcgctat cggccggcgg cgcggtctcc
     1141 tcgcgcggga cgtcccagcc ggggaccgcg atggcgcgct cgcgccggcg gcggcgcacc
     1201 ggaacgatcg cggcaccggc gagcagcgcg gccgcggggc cgagcacgat cagcagcgac
     1261 gaggtcttga acccatcgga ggaggccgcg aactcgagcc ggtcgccgaa cggaaccgtc
     1321 cacacgatcg agccgccgga cacgcgctcg ggatcgcccc gataccggcc gacgcggccg
     1381 ggcaggtcga ggctgactcg gaagcggaac acgtcgcgcg cggcctgacg cagcgcggcc
     1441 cgaagctcct cgcccgcatc ggcgcccggc accagtcgct cgggccgcag gtccaccgag
     1501 ccggtcactt ccgcggaggt gcgaaagaag ctcgacgagt gatcgatgcg gaagtcgcgg
     1561 aacacgctcg ggaacgacag cgcgcggtcg cccgattcgc gctcgacctc gcggagcgcg
     1621 cgctcgagct cgccgggatc gtcgaagctg cgcgagatgt cgatgcgcaa cccgccttgg
     1681 ggccgggtct ggcgcaccgt ccagccggtt ccgccgaact gctcgccggt ctcggcgagc
     1741 tcatcgagtg tggcggagcc ctgctcggtc gagcggacca cctcgatgaa ctccttgtcg
     1801 aaggcgaaga cgatccggaa ggtgccacgt ccgtcgtcgc ccatcgcggt cgtgacgtcc
     1861 acggtcatct gacacgcggc cgtcagcagc gcgagcagcg cggtagcggc gagcagacgg
     1921 atgcggcgga cggtcacggc gcggattcta gccgcgcggc cggttcacag gtcgcggagc
     1981 aacgggatca acgtctcggc gacggcctcg acctgctcgg gcaccgcgat cgcgaacggc
     2041 agcggcgccg gcgccacgat cagctcgtcg gcgcccaggt gcgcgaaggc cgccatgcgc
     2101 tcggcgcact gctcggccgt tcccaccagc gcgccggccg cgaactcctc gagcgaggtt
     2161 ccctcgagca tcgtgccggg cgcccagtcg cggaccgcct cgaagagctt gggcaggtcg
     2221 tcgccgacca gcgtgtagag gccgagcgat cgccgcacgc ccgccgggtc gcggccggcc
     2281 cgctcgcatg cctcgtcgag cgtggcggct ttggccgcgt agtcctcggg cgtccacttc
     2341 cagacggtgt tccagccgtc ggcggcctcg gcgacgatac gcgcggcgcg cgggccgcct
     2401 ttgctgccga tccagatcgg cggccgcgga cgctgcaccg gccgcggctc gttgatcgcg
     2461 tcgcgcaccg cgtagtgctc gccctcgaag gtgaccggac cgccgtccag gagccccccg
     2521 acgatgcgca cggcttcctc gagccgctgc atgcgctccc ccgccgatcc gaacggatag
     2581 ccgtaggcgg cgtactcgtc ttcgtaccag cccgcgccca tgccgatctc cagccgcccg
     2641 ccgctcgcga cgtcgagcgt cgccgcggct ttggccagca tcggcggcgg ccggaacgct
     2701 tcgctgagca cgagcgtgcc gagccggacc cgctcggtgg ccgcggcgat cgcgccgagc
     2761 gcggcgagcg gctcgagcga gccgtgccgg ccgggcggtc cgccgtatcg ggtgatctcg
     2821 agaaagaaat gatccgagac ccacaccgaa tcgtagccga gcgtctccgc gcggcaggcc
     2881 cattcgaccg tggcggcaag gtcggcggaa cggccgtcgg gatacgagaa gtcgtagtgc
     2941 ggcagcgcga gtcccgtctt catgtcgcca agcagcctag ccgacgtagg atgcgcatga
     3001 catggatgca cccgctcgct tcgcgctcgc cgtcaaccgg cccgagccgt cggtggacac
     3061 ggccgaggcc tgcttcctga tcgcggcgaa cgtctacccc gagctcgacg tcgcctccga
     3121 gctccggcgc gtggacgcgc tcgcggtccg ctgccctccg ggcttcgacg gcctgcggcg
     3181 ctacctgttc gatgagctcg gctttcgcgg caacaccgag cgctacgacg acccgcgaaa
     3241 ctcgttcctc aacgacgtgc tcgaccggcg ggtcgggctg ccgatcacgc tgtcgatcct
     3301 ggcgatcgag gtgggccggc gcgtgggcgt gccgctgtcg ggtgtcggca tgcccgcgca
     3361 cttcgtcgtg cgccacgatg ccgagccggg gttgttcgtt gatgccttcg ccggcggcga
     3421 gaccctcgac gcggccggct gccgcgaccg cttccacgag atcgtcggac aggacgtgcc
     3481 gttcgatccg gactggctca agccggtctc gacgaccgac atcctgctgc gcgtgctcgc
     3541 caacctcaag aacgtgtact cgcaaaagac gcaatggcac catgtcgagt gggtcatgcg
     3601 ctgccgcctt tcggtgcccg gctgcccggc cgaggagcgc cgcgatctcg cgcatgcgat
     3661 cggccagcag ggccggttcg aggaagcggc gcgcgagctc gaccttcttg cccggattac
     3721 ccccgatccg gatgcgcgcc acgcgatcac cagcgaggcg atcacgatgc gcgcacagct
     3781 gaactagtcc cggcgaatcc cggaacgtcc tgggccgttc ggcctgtcgc tgccgcgccc
     3841 gggtgcgcga gactgacggt gagccggccg gacgagagcc ggaccagccg agaggaggca
     3901 tcgatgcgcc gccggacctt cgacaccttg atgagcctgg gcgggctcgc catgaccatg
     3961 gtgctgctga tcgcaggatc gctgctgttc gtggggtaca cctacgcgaa caacaccgtc
     4021 acgagccagc tcaaggcgca gagcattttc ttccccgccg ccgacaaccc gcagatgcag
     4081 gacccccgga tcggtccgtt cgtcgctccc tacgccggag agcaggtcgt gaccggccgt
     4141 caggccgagg tctacgcgaa ccacttcatc ggcgtgcatc tggaagacgt cgccgaagga
     4201 aagacctaca gcgaggtcag cacgctcagc cgcgcgaacc ccgacgacgc gacgctggcc
     4261 ggacaggtgc agacgctgtt tcgcggtgag accctccgcg gcgtgctgct gaccgcctac
     4321 gcgttctggc agctgggaca gatcgcgctc tggggttcga tcgcatcctt cgcgctcgcg
     4381 atcgtgatgg cgaccctgac cgtcctcggg ttctggcacc gccgccgggt ggcgcccgag
     4441 gaggagatcc tcgcgccgcc gcgcggcctg gccgaagcgg tctgacgcgc gcatggatcg
     4501 agcccggcgc cgaggcggcg ccgggctcga tcacgcggat cttctacagg tagcgcatca
     4561 cgtcgtcggc gtcgcggatc tcgacgcccg gcgggatcgc ggcggcggcg gggatgcgaa
     4621 gccgcggcca gatggtgatg ttgccgctga tgcgtgtgcc gggctgcagc acgacctcgt
     4681 cgccgagccc gacgtagtcc tcgagcacgg tcggggtctc ggggctggag cgtacctccg
     4741 acatcgagcc gacgtaggcg tcggt
//
LOCUS       DATLFO010000309         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_97455, whole genome shotgun sequence.
ACCESSION   DATLFO010000309 DATLFO010000000
VERSION     DATLFO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97455"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VM841_12465"
     CDS             <1..1423
                     /locus_tag="VM841_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91038.1"
                     /translation="FSWDRTAQTPESAYGLEVMRAGIPGDVASSGRPGIECAAADPND
                     LEPPRTVAGRSHQWGRFADSGGRATRIPSGTYSWRVRDAASTQSSPVDRFVVAGVEGI
                     FDRYHDDVDEEIVYPVNRSPLKVAVAASSERGGQKPVPDGWLDTIVRGLDRWNGIEPS
                     FSFAYDGRCAIVSVPTADPADCNEEIPRPFVQVRSLGERGPLAVTQVTWDRALSGTHF
                     TTADGINLLQGAIVSINDSYAARLWLGANAPASGSYDLESIVAHELGHVAGLGHDYTF
                     PAATMHYRLQEGAMHRIPQQFDRASLRFLYGQSHGGALMPYVKPLQIAEQAALIVRGS
                     TSGTPTLRVASTGELYHEHRFVVAEYLKGTGPAEILVRTPLDSRGNPYEGAATLTTPV
                     EQVLALSTETLGAMDMSPGSAYYVTYWSAGAWWISSDGFAINQADSTFAEPLADLRDR
                     ISAVVAGGPDPHPLPGIPRYVIA"
     gene            1435..1878
                     /locus_tag="VM841_12470"
     CDS             1435..1878
                     /locus_tag="VM841_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91039.1"
                     /translation="MRRIVTVSAVLLLAATLDPALSHTASVRIEQRIRPLLVDGSTRL
                     AVSGSPCRSGRLIEPLPAIAGRYEVECFDPVGRFNLSLEVDDRGEMRAEGWFLIGGLC
                     CPESIGPFSPAVGGGRYRFSDPWDLRLRGLWAGPGSASMPSGGDR"
     gene            1932..3299
                     /locus_tag="VM841_12475"
     CDS             1932..3299
                     /locus_tag="VM841_12475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HVL91040.1"
                     /translation="MRRTFRWLLSMVGLVSMLVPVTPSAAIVAERTPMNVGGLVVSST
                     SVKELKGEGTIIRTYAMLRVTSADREALVGTVIPVVADGGRIGDERTWVEMMPALSKD
                     AEVSLVVIDGSLPYEVSTGSPPEPFQAPGYLTVGEMIESEYNLIYGYGWACPRYLPGS
                     YYHNLTGAPSGARAAVNAGYEAWENDASSWMEFNDAGTTSIRSQSNDGTNAVFWLTDN
                     AGTYLARTWVYANGSRITGFDVQFNTRYSYSTNPNTSQVDIQSVATHEAGHDVGLDHV
                     SNCTQIMGTQWIGGVEYGCLDYGEKHRTLGSDDLAGLRFLTPYNDIRGTFQAQSPSNS
                     TLIMIPGQRYPTDLNPAKFTARFRNTGKTPWGNWSAGSNEIRLNTALPANRTSPFCDS
                     TWISCARPDRATTLVCTGEEITMDWYMKAPLAVGTYDEAFGFVVGSTTVVSSSPRWSE
                     IIAQG"
     gene            complement(3343..>3602)
                     /locus_tag="VM841_12480"
     CDS             complement(3343..>3602)
                     /locus_tag="VM841_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91041.1"
                     /translation="MGSSDDPMLSGNGRVVAFQSDALNLAAGDTNLAVDVFAHAMNAF
                     SCADHPEEGPVSGVVHDTAEIGPAAAPMHSISCDTIARAGA"
BASE COUNT          609 a         1188 c         1188 g          617 t
ORIGIN      
        1 cttcagctgg gaccgaaccg cgcagacgcc ggaaagcgcc tacgggctcg aagtcatgcg
       61 cgcaggcatt cctggtgacg tggcgagcag cggccggccg ggcatcgagt gcgccgcggc
      121 cgatccgaac gacctcgagc cgccgcgaac cgttgcgggc cggtcgcatc agtggggccg
      181 gttcgccgac tcgggtggca gggcaacccg catcccttcc ggcacgtact cgtggcgcgt
      241 gcgtgacgcg gcctcgacgc agtcctcgcc ggtcgatcgc ttcgtcgtcg cgggcgtcga
      301 agggatcttc gaccggtatc acgacgacgt cgacgaggag atcgtctacc cggtgaaccg
      361 atcgcccttg aaggttgccg tggcggcctc gtccgaacgc ggtggccaga aaccggttcc
      421 ggacggctgg ctcgatacga tcgtgcgcgg cctcgaccga tggaacggca tcgagccgag
      481 cttctcgttc gcctacgacg gccggtgcgc gatcgtgagc gttcccacgg ccgatccggc
      541 cgactgcaac gaagagattc ctcgtccatt cgttcaggtg cgcagcctcg gggaacgcgg
      601 tcccctggcc gtgacgcagg tcacgtggga ccgggcgctg tccggaacgc atttcactac
      661 cgccgacggg atcaacttgc tgcagggcgc gatcgtgtcg atcaacgact cctatgccgc
      721 gaggctgtgg ctcggcgcca atgcgccggc gagcggcagc tacgacttgg agagcatcgt
      781 ggcccacgag ctcggccacg tcgccgggct cggtcacgac tacacctttc ccgcggcaac
      841 gatgcactac cggctccagg aaggagccat gcatcggatc cctcaacagt tcgaccgggc
      901 gtcgctgcgg ttcctgtacg ggcagtcgca cggtggcgcg ctgatgccgt acgtcaagcc
      961 gcttcaaatc gccgagcagg cagcgctcat cgtccggggc tcgacgagcg gaactccgac
     1021 gcttcgcgtc gcgtcgaccg gggagctgta tcacgagcac cgtttcgtcg tcgccgaata
     1081 cctcaaagga accggtcccg ccgagatact ggtccgaaca ccccttgatt cgcgcggcaa
     1141 cccgtacgag ggtgcggcga ccctgacgac gcccgtcgag caggttctgg cgctgagtac
     1201 cgagacgctc ggcgccatgg acatgagccc gggatcggcg tactacgtga cctattggtc
     1261 agcgggcgca tggtggatca gcagcgacgg gttcgcgatc aaccaggcag attcgacctt
     1321 cgccgagccg ctggcggacc tgcgcgaccg gatatccgcc gtcgtcgcgg gcggtcccga
     1381 tccacatccc ttgcccggaa tccctcggta cgttatcgcc taggaacgcg tgcgatgagg
     1441 cgcatcgtga ccgtctccgc cgtgttgctg ctcgccgcga ctctcgatcc cgccctgtct
     1501 cacacggcgt cggttcggat cgagcagcgg atccggccgc tgctggtcga cggatccacg
     1561 cggctcgccg tgtcgggcag cccgtgccgt tcgggccggc tcatcgagcc gctgccggcg
     1621 atcgccggtc gctacgaggt cgaatgcttc gatcccgtcg gccgattcaa cctgtcgttg
     1681 gaggtagacg accgcggcga gatgcgtgcc gagggatggt ttctgatcgg ggggctgtgc
     1741 tgtcccgaat cgattggtcc gttcagcccc gcggtcggcg gtggccggta tcgattctcc
     1801 gatccgtggg atttgcggct gcggggcttg tgggccggtc ccggatcggc gtcgatgcct
     1861 tcaggaggtg atcgatgacc gaaagatgag ttcgtattgt cccgcccgat gcgactcgaa
     1921 tggagaacaa gatgagaaga accttccgct ggcttctatc gatggtcggc ctcgtttcga
     1981 tgctcgtgcc ggtcacgccg tcggctgcga tcgtggccga aagaactcct atgaacgtcg
     2041 ggggtctcgt ggtgtcgtcc acgtccgtga aggaactcaa gggcgaagga acgatcattc
     2101 ggacgtacgc gatgcttcgc gtcacgtccg cggaccgaga agcgctcgtc ggcacggtca
     2161 tccccgtggt cgccgacggc ggccggatcg gagacgagcg gacctgggtc gagatgatgc
     2221 cggcgctttc caaggatgcc gaggtctcgc tcgtagtcat cgacgggagc cttccttatg
     2281 aagtctccac cggctctccg cccgagccct tccaggcccc gggctatctc accgtaggcg
     2341 agatgatcga atccgagtac aacttgatct acggatacgg ttgggcgtgc ccgaggtacc
     2401 ttcccggctc gtactaccac aacctcacgg gcgctccttc cggcgcgcgc gcggccgtga
     2461 acgcggggta cgaggcgtgg gagaacgatg cctcgagctg gatggagttc aacgacgccg
     2521 gaacgacgag catccgctcc cagtcgaacg acggcacgaa cgcggtcttt tggctgaccg
     2581 acaacgccgg cacgtacctg gcccgcacgt gggtctatgc caacgggtcc cggatcaccg
     2641 gcttcgacgt gcagttcaac acccggtact cgtactcgac gaacccgaac acgtcgcagg
     2701 tcgacatcca gagcgtggcg acgcacgagg caggccacga cgtcggcctg gaccacgtca
     2761 gcaactgcac gcagatcatg ggaacccaat ggatcggcgg cgtcgagtac ggatgcctcg
     2821 actacggtga gaagcaccgc acgctcggca gcgacgatct cgcgggactt cgtttcctta
     2881 ccccctacaa cgacatcagg ggcaccttcc aggcccagtc cccgtcgaac tccacgttga
     2941 tcatgatccc gggccagcgc tacccgaccg atctgaaccc ggccaagttc accgcgaggt
     3001 tccgtaacac cggcaagacc ccctggggca actggtcggc cggatcgaac gagatccgcc
     3061 tgaacacggc cttgcccgcg aatcgcacca gcccgttctg cgattccacc tggatatcgt
     3121 gcgcgcgtcc ggaccgggct acgacgctgg tgtgtaccgg cgaagagatc acgatggact
     3181 ggtacatgaa ggcgcccctc gccgtcggga cctacgacga agcattcggt tttgtcgtgg
     3241 gatcgacgac ggtggtgtca tcatcgccgc ggtggtccga gatcatcgcg caaggataga
     3301 gagcacgggc gttgacgcgg gccggggcgt cgtcccggcc cgctacgcgc ccgcgcgtgc
     3361 gatggtgtcg cacgaaatcg aatgcatcgg cgcggcggcc ggtccgatct cggccgtgtc
     3421 atggaccacg ccggacaccg gaccctcctc cgggtgatcg gcgcaggaga aggcgttcat
     3481 cgcatgggcg aacacatcga cggccaggtt ggtgtcgccg gcggccaagt tcagcgcgtc
     3541 ggactggaac gcgaccacgc gcccgttgcc cgacagcatc gggtcgtcgc tgcttcccat
     3601 cg
//
LOCUS       DATLFO010000310         2340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_38546, whole genome shotgun sequence.
ACCESSION   DATLFO010000310 DATLFO010000000
VERSION     DATLFO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38546"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VM841_12485"
     CDS             <1..558
                     /locus_tag="VM841_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91042.1"
                     /translation="HHPLYPGAGVVHRVMYTKEQPLACGGAPGNWGFVDFNGGNNSTG
                     EMRRWIRDGFNDNAIAVGDCNADNLTPAEWCPGNTGAASNSISNALRTIVGTTFPLPL
                     FDQVRGAGSNAEYRVAGFLGVILRGYRVTGPEAGRYLDLEFTSLIGSGRCCPVDGSAV
                     VDGGVRTVRLCSVDHDPLSVATRCA"
     gene            590..1363
                     /gene="cpaB"
                     /locus_tag="VM841_12490"
     CDS             590..1363
                     /gene="cpaB"
                     /locus_tag="VM841_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVL91043.1"
                     /translation="MNSIIGNRRMLAVLVALLLAALATASLLSYMRGVERRAKDGEVL
                     VSVVVAKGQIPAGTSGAAAGSNGLLGLENVPRRLVADGAMLDVADLQDKVAATTIRTG
                     EQIVTGRFVVPAELTGALPIPADRQAMSLEVAVPHGAGGFVQPGSRVSILAKFQLKMV
                     NGESVDVADYLIQNVPVLAVGSSVVSTTPQERPAGAPAEEEAAPSRQDRIVLTLALLP
                     TDTSRLALAMTDGVIFVTLLPPGQKPVTVPARTFANEFE"
     gene            1378..>2340
                     /locus_tag="VM841_12495"
     CDS             1378..>2340
                     /locus_tag="VM841_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HVL91044.1"
                     /translation="MILVYDPDPGFTAAIGAALRRTDLKPAETFDDFDRAVGSGKISA
                     VVVGPDAGEGTALSLAAHLQQHAPDIGLIMVEHEVTAEVLQAALRAGVRDVLPVAFKA
                     TSLSEAMQRAESRSRRPHAAARDPEAGDGAALGGRIITVFSPKGGVGKSCVATNLAVL
                     LAGAGHNVAAVDLDLQFGDLAIMMQLFPSRTIYDAATAAGEMDPETLIEYLTPHRSGL
                     GLLPAPLEPGLAENVSADAAQKVLVGLRTAGRTVVVDTPPWFNDHVLQALDVSDEMVL
                     VATMDVPSIKNLKVALQTLAMLGYPRERLRLVLNRADSKVGLSLP"
BASE COUNT          362 a          825 c          806 g          347 t
ORIGIN      
        1 catcacccgc tgtatccggg ggcgggcgtg gtgcaccgcg tgatgtacac gaaggagcag
       61 ccgcttgcct gcggcggtgc tcccggcaac tggggcttcg tcgacttcaa cgggggaaac
      121 aactcgaccg gcgagatgcg acgctggatt cgggacggct tcaacgacaa cgccatcgcc
      181 gtcggcgact gcaacgccga caacctgact cccgccgagt ggtgcccggg caacaccggt
      241 gccgcatcga acagcatctc caacgccctc cgcacgatcg tcggcacgac ctttccgttg
      301 ccgttgttcg atcaggtccg cggagcgggc agcaacgcgg agtaccgggt cgccggcttc
      361 ctcggcgtga tcctgcgcgg ctaccgggtc acgggcccgg aggcgggccg gtacctcgat
      421 ctcgaattca cgtcgctcat cggcagcggc cggtgctgcc ccgtcgacgg ctcggccgtc
      481 gtggacggcg gcgtccggac cgtgcgtctg tgctcggtcg atcacgatcc gctttctgtc
      541 gcgacccggt gcgcgtagac cgccgccggg tggaaaggag tcgcagctca tgaactcgat
      601 catcgggaac aggaggatgc tggccgtgct ggtcgcgctt ctgctcgccg cgctcgcgac
      661 ggcatcactg ctgtcgtaca tgcgtggggt ggagcgacgc gccaaggacg gggaagtgct
      721 ggtctcggtc gtcgtggcga agggccagat ccccgccggg acgagtggcg cggcggcggg
      781 cagcaacggg ctgctcgggc tcgagaacgt gccgcggcgg ctcgtcgccg acggcgccat
      841 gctcgacgtc gccgaccttc aggacaaggt cgccgccacc acgatccgca cgggcgagca
      901 gatcgtcacc ggccggttcg tcgtaccggc ggagctcacg ggcgcgctgc cgatccccgc
      961 cgatcggcag gcgatgtcac tcgaggtcgc ggtgccgcac ggcgccggcg gcttcgtcca
     1021 gcccggcagc cgcgtgtcga ttctggcgaa gtttcagctc aagatggtca acggcgaatc
     1081 ggtggacgtc gccgactacc tgatccagaa cgtgccggtc ctcgccgtcg gatcgtcggt
     1141 ggtctccacg acgccgcaag aacggcccgc cggcgcgccg gccgaagaag aagccgcccc
     1201 gagccggcag gaccgcatcg tgctaacgct cgcgctgctg cccacggaca cgtcccggct
     1261 tgcgctcgcg atgaccgacg gcgtgatctt cgtgacgctg ctgccgccgg gccagaagcc
     1321 cgtgacggtt cctgcccgca cgttcgcgaa cgaattcgag tgaaaggcgg gatgacgatg
     1381 attctcgtat acgacccgga tcccggcttc accgcggcga tcggcgcggc gctgcgccgg
     1441 accgatctaa agccggccga gacattcgac gacttcgacc gcgccgtcgg ctccggcaag
     1501 atcagcgccg tcgtcgtcgg tccggacgcc ggcgaaggaa ccgcgctgtc actcgccgcg
     1561 cacctgcagc agcacgcccc cgacatcggc ctgatcatgg tcgagcacga ggtcacggcc
     1621 gaggtcctgc aggccgcgct gcgagccggc gtgcgcgacg tgctccccgt ggcgttcaaa
     1681 gccacctcgc tgtcggaggc aatgcaacgc gccgaatccc ggtcgcggag gccacacgcc
     1741 gcggcccgcg atcccgaagc cggcgacgga gccgcgctcg gcggccggat catcacggtg
     1801 ttcagcccca aaggcggcgt cggaaagagc tgcgtggcga cgaacctggc ggtcctgctc
     1861 gccggcgccg gtcacaacgt cgccgcggtc gatctggacc ttcagttcgg cgatctcgcg
     1921 atcatgatgc agctgttccc gtcgcgcacg atctacgacg cagccacggc cgccggcgag
     1981 atggatcccg agacgctgat cgagtacctg acgccccacc ggtccggcct cgggctgctg
     2041 cccgccccgc tcgagccggg gctggccgag aacgtctcag ccgacgcggc gcagaaggtg
     2101 ctcgtcggcc tccggaccgc cggccgcacg gtcgtggtgg acacaccgcc gtggttcaac
     2161 gaccacgtgc tccaggcgct cgacgtgagt gacgagatgg tgctcgtcgc gacgatggac
     2221 gtgcccagca tcaagaacct caaggtcgcg ctgcagacgc tggcgatgct cggctatccg
     2281 cgcgagcggc tgcggctggt cctcaaccga gccgacagca aggtcggcct gtccctgccc
//
LOCUS       DATLFO010000311        11908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_77611, whole genome shotgun sequence.
ACCESSION   DATLFO010000311 DATLFO010000000
VERSION     DATLFO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77611"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..2517)
                     /gene="pheT"
                     /locus_tag="VM841_12500"
     CDS             complement(118..2517)
                     /gene="pheT"
                     /locus_tag="VM841_12500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HVL91045.1"
                     /translation="MKVPFSWLRELTPVDATTDDIAARLAMLGFAVDEIHSTGNEIRD
                     VVVGKVLDVRDHPSSSKPLVLVQADVGQGEPLHIVCGARNFSAGDLVPVAVPGARLPG
                     GFEITRRKLAGQESNGMLCSARELGIGDDHSGIMVLDEGAEVGDDVVATLGLADTIFD
                     IDVTPNRPDGLSVLGIAREIAAAYGLPVGEGAADVAEGGPAAETLASVAVEDAKGCPR
                     YVARVATGLTNGSSPWWMQRRLLACGMRPISAIVDITNYVLLERGHPLHAFDLATLEG
                     RAIVVRRARKGETMRTLDGVERTFVRDDVLICDTAKPVAVAGVMGGEDSEVSAGTTGI
                     LLESALFDATRVLRTARRLGLRTEASVRFERGVDPAMPRRAADRAMALVSQICGGAVA
                     PGAIDAGEAPKPRKPVRLSVATANARIGISATADEMTAALTALGCEVSGSSRTALRVT
                     PPSWRGDIAIEEDLIEEVARVHGYDNVPVTLPAGGGVGALTTEQRLRRTARRALLGAG
                     LSEAYTLSLISPAFADRIGLGAGHPWREVLPVTNPLSEDESVLRPSLLPGLLLAARHN
                     IARRQPSVMLFEIGMTFTPSREELPVETRQAAWVMAGAASADWHQPERPLDFYDAKGA
                     FESLAQALGVANAAALPGQGPEPFHPVRFADLFVGSRLAGVVGELHPRVIDALDLPAR
                     TAAGVVDLSMLIEAAVPARDLPLPRFPAVSRDLAVVAPNETTAAEIASALAEAAGPLL
                     ASIDPFDVYRGGQVAEGKRSVAFALQFRDPERTLTDDEVDAAMSRAIDAAAGRGWAIR
                     S"
     gene            complement(2514..3557)
                     /gene="pheS"
                     /locus_tag="VM841_12505"
     CDS             complement(2514..3557)
                     /gene="pheS"
                     /locus_tag="VM841_12505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HVL91046.1"
                     /translation="MSDPITVIEQQTAVALERIAGATTTAELREAEIAALGRKAPMSQ
                     VKASLAQATPEQRKEIGRALNEARAAIESALAEREGTFRAAEEAAALQRDRIDVTLPG
                     RRTPPGRPHPISMMTEWLVDIFVGMGFNVAEGPEIETDWYNFEALNMHEDHPARTMQD
                     TMFVQGAQSTLVARTHTSPVQIRSMLASEGPLYVVAPGRTYRRDPFDATHSPVFHQIE
                     GLAVDRDISLADLKGVLTEFARAVFGPKQKVRLRPSYFPFTEPSAEVDVVCPRCGGNG
                     CGPCSRSGWMEIMGAGMVHPNVLRAGEYDPAAWSGFAFGMGIERIAMTAWPVGDIRNL
                     FENDLRFLGRFAG"
     gene            complement(3554..4633)
                     /locus_tag="VM841_12510"
     CDS             complement(3554..4633)
                     /locus_tag="VM841_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL91047.1"
                     /translation="MSDTPVLAAIPDAVVVIEEGGALSYLNPAAERLSGWDAAEAGGR
                     HYGEVFPLRDAAGFLVHERYDPFASRLSTVTRSPEREYLLRRRDGSEVWVAVRSSFER
                     NERGRVVRVVAALRDIGRRRRLDRAKSDLISTVSHEIRSPLTSVKGFTSTLLHRWERF
                     TDEQKQLMLRTINADADRVTRLLTDLLDVSRLEAGRLELKRQKVDVAAIARNVVERLQ
                     IDHDDRPLSARFDAVPEVWADPGKVEQVIVNLVENALKYAPRGGVVVSGTTEDDGIVV
                     RVRDEGEGIDPRHVPHLFTKFYRRGHGERTGGTGLGLYICHGIVGAHGGRIWVERSDA
                     SGTVFAFTLPTGEGSWVGAGGGAST"
     gene            complement(4630..5376)
                     /locus_tag="VM841_12515"
     CDS             complement(4630..5376)
                     /locus_tag="VM841_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HVL91048.1"
                     /translation="MISSRANAAVKSTVRLRKRREREKRGVFLVEGHRAVSAAVAKGI
                     KLLDVFHTEPALRHREPLLRSISGATVRSVTPEIMAHLTGHDHPPDVLAIAPLRPSSI
                     KAAVKGDLIVILSGVRGPGVAGGILAAAAAADASGVIVLGSTTDPFGPACVRAAAGAH
                     FALAVAPCEDAAAAAAAAGQRTVVVLSEDGSPVCSENMRPPLALVVAEGPLPEAFSTA
                     RRVSVPAGSAGVAAGLVARAAIALHEARHP"
     gene            complement(5435..5839)
                     /gene="rplT"
                     /locus_tag="VM841_12520"
     CDS             complement(5435..5839)
                     /gene="rplT"
                     /locus_tag="VM841_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVL91049.1"
                     /translation="MARVKRSVGGLKKRRAVLEQAKGYRGSRGKHYRAAREQVLHSMR
                     YAFNDRRARKGDFRRLWIARINAATREQGLSYNRFINGLRLAEVEIDRRMLAEIAVSD
                     PSAFRQLVAVAKAGLEKAGPAGASQPASDAQA"
     gene            complement(5841..6035)
                     /gene="rpmI"
                     /locus_tag="VM841_12525"
     CDS             complement(5841..6035)
                     /gene="rpmI"
                     /locus_tag="VM841_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVL91050.1"
                     /translation="MPKMKTHRSTAKRVKRTGTGKLVRKRANRSHILEKKSSKRKRAL
                     GQKALVSDADAPRLNRLLGG"
     gene            complement(6068..6694)
                     /gene="infC"
                     /locus_tag="VM841_12530"
     CDS             complement(6068..6694)
                     /gene="infC"
                     /locus_tag="VM841_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVL91051.1"
                     /translation="MAVEPRVNERIRSPEVRLVGPDGQQIGIISLPDAMKIARELDLD
                     LVEVAPQARPPVCRLLDYGKYKYEQALKAKEAKKKQSQVVVKEMKMRPKIDPHDYATK
                     KGHIVRFLNQGHKVKLTIMFRGREMAHTELGERILRRLTADLQELANVETPSKLDGRN
                     MTMVFAPLKKKVQPKAEVPAGLVEEIAVDEIAVEQDAPETAAAEPASE"
     gene            complement(7016..7216)
                     /locus_tag="VM841_12535"
     CDS             complement(7016..7216)
                     /locus_tag="VM841_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVL91052.1"
                     /translation="MAEGTVKWFSNEKGYGFISPAEGDDVFVHHSAIQMDGYRSLAEG
                     QKVEFEIVDGPKGKQAANVRPA"
     gene            complement(7356..8234)
                     /gene="hisG"
                     /locus_tag="VM841_12540"
     CDS             complement(7356..8234)
                     /gene="hisG"
                     /locus_tag="VM841_12540"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HVL91053.1"
                     /translation="MLRLVIPKGSLEPQTLAVLEAADLTVRRGSDRAYHGTIDDPRIE
                     RVSILRPQEIPRYVQEGFFDIGITGLDWVAETDADVEVLGELAYSGRAGGGVVKIVLA
                     VPNDHAAQSLADLPPDSRISTEYPELTKRAFEAAGIPVRVYLSYGATEAKVPEIVDAV
                     VELTETGSTLKRNGLKIIDVLLESHTVVIANKTAAEDPEKRRAMEDLLTLIRGATSAR
                     GRVLVKMNVSEDNLEKVLEILPAMKAPTISKLTHEHDYAIETVVEKTRINTLIPELLQ
                     RGASDIIELPISKIVP"
     gene            complement(8267..8737)
                     /gene="ribH"
                     /locus_tag="VM841_12545"
     CDS             complement(8267..8737)
                     /gene="ribH"
                     /locus_tag="VM841_12545"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVL91054.1"
                     /translation="MRSADEPIDASEFTFAIVVSEFHHEITERLLAGAKRCLAEHRAP
                     EPDVYSVPGAFELPLAAQLAAETERYDAVICLGCVIRGETAHFEYVAGEAARGIGEAA
                     RETGIPIAFGVLTSDTQEQAEARATDGRDNKGWEAARTAVEMASFVGSLPEQPE"
     gene            complement(8724..9974)
                     /locus_tag="VM841_12550"
     CDS             complement(8724..9974)
                     /locus_tag="VM841_12550"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HVL91055.1"
                     /translation="MFATIDEALEELKAGRAVIVVDDADRENEGDFVMAAEKVTPDWI
                     NFMATHGRGLICMPVVGERLDALGIPPMVPDNADTNETAFAISIDLSDGGTGISAHDR
                     ASTVLKVLDPQAEPADFRKPGHIFPLRSQPGGVLKRAGHTEAAVDLSVLAGMYPAGVI
                     CEIMNPDGTMARLPELIEVAREHNLKLISIADLIAYRRTIERLVRREAEASLPTPFGT
                     FTVVGYESMVDDRHHIALTLGDIGDGQNVLTRMHSECLTGDVFGSLRCDCGTQLEDAM
                     KQVASEGRGVIVYLRGHEGRGIGLLHKLKAYRLQEDGQDTVEANVSLGFAPDPRDYGI
                     GAQILVDLGVRSMRLLTNNPTKRAGLEGYGLTIAERVPLQTKPTEHNIAYLRAKKAKL
                     GHLLDLGENGQSEGEAADRPDAQR"
     gene            complement(9995..11008)
                     /locus_tag="VM841_12555"
     CDS             complement(9995..11008)
                     /locus_tag="VM841_12555"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVL91056.1"
                     /translation="MFTGIVEGMGRVIRLLRRGGGARLEVRAPGMLDGLKIGDSVAVN
                     GACVTVTAIEGDAFACDLVPETLARTTLGMLQPHEEINIERSMRADARFDGHIVQGHI
                     DVVGVVRSRRKVGAQEMLEINVPFELTRYLAPKGSVAVDGVSLTVVDVNRDRFRVALI
                     PHTIGMTTLGKKIQGSTVNVEVDVLSKYVERHIAARSPRPQPSLAELVKMPLRGDETG
                     PVPAVSASAPPPAGPVPGMTAVTPGPAAATRPSPKVSSKPPAKPKAPVKPAKPAAKAK
                     PAAKAGAPAKPPVKARPALGKKAPAKAVSAARRPAPAKSSKTTRAASTKIKKPAATRS
                     GRR"
     gene            complement(11038..>11908)
                     /gene="ribD"
                     /locus_tag="VM841_12560"
     CDS             complement(11038..>11908)
                     /gene="ribD"
                     /locus_tag="VM841_12560"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HVL91057.1"
                     /translation="PCTVDGRTPPCAPAVAAAGIRRVVIGTADPNPRVNGAGIRFLHD
                     AGIDVEAGVLELDCRRMVEAFAKHVLTGRAFVTGKIAATLDGRAAAVDGSSKWITGPT
                     ARRDAHRLRGSVDAVLVGVGTVLKDDPRLTVRLRGFKGRQPLRVVLDSSGRTPLQSSV
                     LNAEAPTLVAVTDKAPEDAVAALRAHGAEVWRAPARDGRIDLAATLDFLGGRDVMEVM
                     IEGGPAVLGDAVEHGLVDRYVFYLAPKLLGSSGLPAIAGFVTPTIAEARELVVHSVRH
                     AGADLRVEAYPRP"
BASE COUNT         1693 a         4145 c         4193 g         1877 t
ORIGIN      
        1 gctatagccc gaggcaccca cgacggcggc acgataggac acgacgaccc ctccacggct
       61 tcaagtcgca tgagaataca ggtatgtgca tatctatgca accggccgga cggccggtca
      121 ggagcggatc gcccagccgc ggccggcggc cgcgtcgatg gcccggctca tcgcggcgtc
      181 gacctcgtcg tcggtcaggg tccgctccgg gtcacggaac tgcagcgcga aggcgacgct
      241 gcgcttgccc tcggccacct gtccgccccg gtagacgtcg aagggatcga tcgaggcgag
      301 cagcggcccc gccgcctccg ccagcgccga ggcgatctcg gccgcggtcg tctcgttggg
      361 cgcgaccacc gcgagatcgc ggctcacggc cgggaagcgc ggcagcggaa ggtcccgggc
      421 gggcacggcg gcctcgatca gcatcgacag gtccaccacc cctgcggccg tgcgcgcggg
      481 aaggtcgagc gcgtcgatca cgcgcggatg gagctccccc acgaccccgg caagccgcga
      541 gcccacgaac agatccgcga agcgcaccgg gtggaacggc tcgggcccct ggcccggcag
      601 cgcggcggcg ttcgcgacgc cgagcgcctg cgccagcgac tcgaacgctc ccttcgcgtc
      661 gtagaagtcg agcggccgct cgggctgatg ccagtccgcg gacgcggccc cggccatgac
      721 ccaggcggcc tggcgggtct cgaccgggag ctcctcccgc gacggcgtga aggtcatgcc
      781 gatctcgaag agcatcaccg aaggctggcg gcgcgcgatg ttgtgccggg cggccagcag
      841 caggccgggc agcagcgacg ggcgcagcac cgactcgtcc tcggagagcg ggttggtcac
      901 cgggagaacc tcgcgccacg gatgaccggc gcccaggccg atccggtcgg cgaacgcggg
      961 cgagatgagc gacagcgtgt aggcctcgga cagtccggcg ccgaggagcg cgcggcgcgc
     1021 ggtgcggcgc agccgctgct cggtcgtgag cgcgccgacg ccgccgccgg ccggcaacgt
     1081 caccggcacg ttgtcgtagc cgtggacgcg cgcgacctcc tcgatcaagt cttcctcgat
     1141 cgcgatgtcg ccgcgccagc tcggcggggt cacgcgaagc gccgtgcgcg acgagcccga
     1201 gacctcgcag ccgagcgccg tgagcgcggc agtcatctcg tcggccgtgg ccgagatgcc
     1261 gatgcgcgcg ttggccgtgg cgaccgacag acgcaccggc ttgcgcggct tcggcgcctc
     1321 gcccgcatcg atcgcgcccg gcgcgacggc gcccccgcaa atttgggaca cgagcgccat
     1381 ggcgcggtcc gcggcacgcc ggggcatcgc gggatcgacg ccgcgctcga atcgcacgga
     1441 cgcctcggtg cgcaggccca gacggcgcgc ggtccgcagg acacgcgtgg cgtcgaacag
     1501 cgcggactcg agcaggatcc cggtggtccc ggccgagacc tcgctgtcct cgccgcccat
     1561 cacgccggcc acggcgaccg gtttggccgt gtcgcagatc agcacgtcgt cccggacgaa
     1621 ggtccgctcg acgccatcga gcgtgcgcat ggtctcgccc ttgcgcgcgc ggcgtaccac
     1681 gatcgcccgg ccttccagcg tggcgaggtc gaaggcgtgc agcggatggc cgcgctccag
     1741 gagcacgtag ttggtgatgt ccacgatcgc cgagatcggc cgcatcccgc aggccagcag
     1801 ccgccgctgc atccaccacg gggaggagcc gttggtcagg ccggtggcga cgcgggcgac
     1861 gtatcggggg caacccttgg catcttcgac ggcgacgctc gcgagcgttt cggccgccgg
     1921 gcccccctcg gcgacgtcgg ccgcgccttc gccgaccggc aggccgtacg cggcggcgat
     1981 ctcgcgtgcg atgccgagca cagacagccc gtcgggccgg ttgggcgtga cgtcgatgtc
     2041 gaagatcgtg tcggcgagcc cgagcgtggc gacgacgtcg tcgccgacct cggccccctc
     2101 atccagcacc atgatcccgg agtggtcgtc gccgatgccg agctcgcgcg ccgagcacag
     2161 catgccgttg gactcctgac cggccagctt gcgacgtgtg atctcgaagc cgcccggcag
     2221 tcgcgcgccc gggacggcca cggggaccag gtcgcccgcg gagaagttgc gcgccccgca
     2281 cacgatgtgg agcggctcgc cctggccgac gtcggcctgc acgagcacca gcggcttgct
     2341 cgacgacgga tgatcgcgca cgtcgaggac cttgccgacc acgacgtcgc gaatctcgtt
     2401 gccggtcgaa tggatctcgt ccacggcgaa gccgagcatc gccagccgtg cggcgatgtc
     2461 gtcggtcgtg gcgtcgaccg gcgtgagctc gcggagccag gaaaatggaa ccttcatccg
     2521 gcaaaccgtc ccaggaagcg aaggtcgttt tcgaacaggt tgcggatgtc cccgaccggc
     2581 caggcggtca tcgcgatccg ctcgatcccc atgccgaacg cgaagcccga ccacgcggcc
     2641 gggtcgtact cgccggcgcg cagcacgttc ggatgcacca tgccggcgcc catgatctcc
     2701 atccagccgc ttcgcgagca gggtccgcat ccgttgccgc cgcagcgggg acagaccacg
     2761 tcgacctcgg ccgacggctc ggtgaacggg aagtacgagg ggcgcagccg caccttctgc
     2821 ttcggtccga agaccgcgcg ggcgaactcg gtcagcacgc ccttgaggtc ggccagcgag
     2881 atgtcgcggt ccacggcgag gccttcgatc tgatggaaga ccggcgaatg cgtcgcgtcg
     2941 aacgggtcgc ggcggtaggt gcggccgggc gccacgacgt agagcgggcc ctcgctggcg
     3001 agcatcgagc ggatctggac cggcgaggtg tgggtgcgcg cgaccagcgt cgactgcgcg
     3061 ccttgcacga acatcgtgtc ctgcatcgtt cgggccgggt gatcctcgtg catgttgagc
     3121 gcttcgaagt tgtaccagtc cgtttcgatc tcggggcctt cggcgacgtt gaagcccatg
     3181 ccgacgaaga tgtccacgag ccattcggtc atcatcgaga tcggatgcgg gcggccgggc
     3241 ggggtgcggc ggccgggaag tgtgacgtcg atccggtcgc gctgcagagc ggcagcctcc
     3301 tcggccgcgc ggaaggtacc ttcgcgctca gccagcgcgg actcgatggc ggcgcgggcc
     3361 tcgttgaggg cgcggccgat ctccttgcgc tgctcgggcg tggcctgtgc gagcgacgcc
     3421 ttcacctggg acatcggggc cttgcggccg agcgccgcga tctcggcctc gcgcaactcg
     3481 gcggtggtgg tcgcgccggc gatccgttcc agcgcaacgg cggtctgctg ctcgatgacg
     3541 gtgatcgggt cgctcacgtg ctcgctccac ctccggctcc gacccacgag ccttcgccgg
     3601 tcggaagcgt gaacgcgaac acggtaccgg atgcgtcgga tcgttcaacc cagattcgtc
     3661 cgccgtgggc gccgacgatg ccgtgacaga tgtagaggcc gagccccgtt cctccggttc
     3721 gctcgccgtg cccgcggcgg tagaacttcg tgaacagatg gggcacgtgc cggggatcga
     3781 tgccctcgcc ttcgtcgcga acccgcacga cgatgccgtc gtcctcggtc gttccgctca
     3841 ccaccacgcc accgcgcggc gcatacttga gcgcgttctc gaccaggttc acgatcacct
     3901 gctcgacctt gccgggatcg gcccacactt ccggcaccgc gtcgaacctg gcggacagcg
     3961 gccggtcgtc gtggtcgatc tgcagccgct cgacgacatt gcgcgcgatc gccgcgacgt
     4021 ccaccttctg ccgcttgagc tcgagccgtc ccgcctccag ccgcgacacg tcgagcaggt
     4081 cggtcaggag ccgggtgacg cggtccgcgt cggcgttgat cgttcgcagc atgagctgct
     4141 tctgctcgtc ggtgaaacgc tcccaccggt gcagcagcgt cgaggtgaag cctttgaccg
     4201 acgtcaacgg agatcggatc tcgtgcgaga ccgtcgagat caggtcggac ttggcacggt
     4261 cgagccggcg ccgccgtccg atgtcgcgga gcgcggcgac gacccgcacc acccggccgc
     4321 gttcgtttcg ctcgaacgac gagcgcaccg cgacccacac ctcggagccg tcccggcggc
     4381 gaagcaggta ctcgcgctcg gggctgcggg tgacggtcga caggcggctc gcgaacgggt
     4441 cgtagcgctc gtgaaccagg aaccccgccg cgtcgcgcag cgggaagacc tcgccgtagt
     4501 gccgtccgcc cgcctcggcc gcgtcccagc cggacagccg ctcggccgcc gggttgaggt
     4561 aggacagcgc gccgccttcc tcgataacga cgacggcatc ggggatcgcg gcgagcaccg
     4621 gcgtgtcgct cacggatgcc tcgcttcgtg aagcgcgatc gcggcgcgcg cgaccaggcc
     4681 ggcggcgaca cccgcgctgc cggcgggaac cgagacgcgc ctcgcggtcg agaaggcctc
     4741 gggaagcggg ccttcggcga cgacgagcgc gagcggtgga cgcatgtttt cggagcatac
     4801 cgggcttcca tcctccgaca gcaccaccac ggttcgctgc ccggccgcgg cggcggccgc
     4861 ggccgcgtcc tcgcacggtg caaccgcgag agcgaagtgc gcgcccgcgg cggcccgcac
     4921 gcaggccggg ccgaacggat cggtcgtgct accgagcacg atgacaccgg aagcgtcggc
     4981 ggcggcggcc gccgcaagga tgcccccggc cacgcccggc ccacgcacgc ccgacaggat
     5041 cactatcaga tcgcccttga ccgcggcctt gatcgaggag gggcgaagag gcgcgatcgc
     5101 gagcacgtcg ggcggatggt cgtggccggt caggtgcgcc atgatctcgg gtgtgaccga
     5161 ccgcaccgtc gcaccggaga tggatcgcag cagcggctcg cgatgacgca gggccggctc
     5221 ggtgtggaac acgtcgagca gcttgatccc cttcgcgacc gcggccgaga ccgcgcggtg
     5281 gccctcaacg aggaacacgc cgcgcttctc gcgctcccgg cgcttgcgca gccggacggt
     5341 gctcttgacc gcggcgttgg cccggctcga gatcacgcga tcctcctgaa acgggtcggg
     5401 ggcctcggac gaggcccccg aacgtcgatg cggtctacgc ctgggcgtcg gaggcgggct
     5461 gcgaggcgcc ggccggaccg gccttttcga gcccggcctt ggcgaccgcg acgagctggc
     5521 ggaacgcgga cgggtcgctc accgcgatct cggccagcat ccggcggtcg atctcgacct
     5581 cggcgaggcg aaggccgttg atgaagcggt tgtagctcag gccctgctcg cgggtcgcgg
     5641 cgttgatgcg tgcgatccac agccgccgga aatcgccctt gcgggcgcgc cggtcgttga
     5701 acgcgtaccg catcgagtgc agcacctgct cgcgagccgc gcgatagtgc ttgccgcgcg
     5761 agccccggta gcctttggcc tgctcgagga cggcgcggcg cttcttgagg ccgccgacgg
     5821 agcgtttgac tcgtgccatg tcagcctccc aggagccggt tcaggcgagg tgcgtccgcg
     5881 tccgacacga gcgccttctg ccccagcgcc cgcttcctct tgctcgactt cttctcgagg
     5941 atatggctgc ggttcgcgcg cttgcgaacc agcttgccgg tccccgtccg cttgaccctc
     6001 ttcgcagtcg agcggtgcgt cttcatcttc ggcatggtcg tgcgtccctt cgtgtgtgtg
     6061 ctgcgagcta ctccgaggcg ggctcggccg ctgccgtctc cggcgcgtcc tgctccaccg
     6121 cgatctcgtc caccgcgatc tcctcgacga ggccggcggg gacctctgcc ttgggctgta
     6181 ccttcttctt gagcggggcg aacaccatcg tcatgtttcg gccgtccagc ttcgacggcg
     6241 tctcgacgtt cgcgagctcc tgcaggtccg ccgtgaggcg ccgcaggatc cgctcgccta
     6301 gctcggtgtg cgccatctcg cgtccgcgga acatgatcgt gagcttgacc ttgtggccct
     6361 gattcaggaa ccgcacgatg tgacccttct tggttgcata gtcgtgcggg tcgatcttcg
     6421 gccgcatctt catttccttc acaaccacct gcgactgctt cttcttggcc tctttggcct
     6481 tgagcgcctg ctcgtacttg tacttgccgt agtcgaggag gcggcagacg ggcggccgtg
     6541 cttgcggcgc gacttccacc aggtccaagt ccagctcccg cgcgatcttc atcgcgtcgg
     6601 ggagcgagat gattccgatc tgctgaccgt ccggcccgac gaggcgtacc tcgggcgaac
     6661 ggatccgctc gttcacgcgc ggttcgacgg cgatggagcg atcacctttc ctttcgtctc
     6721 gtccgagccg cgtccgggcc ggcccgacct tccaatcctc tcgaaaaaga aaaaaccgga
     6781 cgcccaagca gggacgcccg gcagcatgcc gtgctccgca accgctcctg gctcccgtgc
     6841 ttcttcggcc ggagggtgag gggccgatga tgcgagaccc ctgcttgtgc ccggacagtg
     6901 taccgggacc cggccggggc tgtcgacgac cccgttgcgg gggccggccg ggcgggaaag
     6961 ccgacggccc cgcggtgttc cgcgaggccg tcgccctcta gggttttgct ccgagctagg
     7021 caggacgaac gttggccgcc tgctttccct tcgggccgtc cacgatctcg aactcgacct
     7081 tctggccctc ggcgaggctt cggtagccgt ccatctggat cgccgagtgg tggacgaaca
     7141 cgtcgtcgcc ctcggccggc gagatgaagc cgtagccctt ctcgttgctg aaccacttca
     7201 cggtgccttc tgccaacttc ctgccctcct cggggccgtc ccgcccaccg aggttcgatg
     7261 ggaaaacgta ccgaaacgac cctggaaacg agcgcagggt agcacggcca aaatcgggcg
     7321 agcaaggacc gggcgcgcgg tcgaaagggc cgaagctacg gcacgatctt ggagatcgga
     7381 agctcgatga tgtcgctggc tccgcgctgc agcagctccg ggatcaaggt gttgatccgg
     7441 gtcttttcga cgacggtttc gatggcgtag tcgtgctcat gggtaagctt cgagatggtc
     7501 ggagccttca ttgccggcag gatctccagc accttttcga ggttgtcctc ggagacgttc
     7561 atcttgacca ggacccggcc gcgagccgag gtggcgccgc ggatcagcgt gagcagatcc
     7621 tccatggcgc ggcgcttctc ggggtcctcg gcggcggtct tgttggcgat gaccacggtg
     7681 tgggactcga gcagcacgtc gatgatcttg aggccgttgc gcttgagcgt ggagccggtc
     7741 tcggtgagct cgacgaccgc gtcgacgatc tcggggacct tggcttcggt cgcgccgtag
     7801 gacaggtaca cgcgcaccgg gatgcccgcc gcctcgaagg cgcgcttggt cagctccgga
     7861 tactcggtcg agatgcggct gtccggcgga agatcggcca gcgactgcgc ggcatggtcg
     7921 ttggggacgg cgagcacgat cttgaccacc ccgcccccgg cgcggcccga gtaagcgagc
     7981 tcgccgagga cttcgacgtc ggcgtcggtc tcggcgaccc agtccagacc cgtgatgccg
     8041 atgtcgaaga atccttcctg cacgtagcgc gggatctcct gcggccgcag gatcgagacg
     8101 cgttcgatcc gcggatcgtc gatcgtgccg tgatacgcgc gatcggagcc gcgccgaacc
     8161 gtcaggtccg cggcctcgag caccgcgagc gtctgcggct cgagagatcc tttggggatg
     8221 accaagcgaa gcatggcgcc gatgctagca gagtcacacg agccgattat tccggctgtt
     8281 ccggaaggga gccaacgaac gaggccatct cgacggcggt ccgcgccgcc tcccatccct
     8341 tgttgtctcg cccgtcggtc gcgcgcgcct cggcctgctc ctgggtgtcc gaggtgagca
     8401 caccgaaggc gatcgggatg ccggtctcgc gcgccgcctc gccaatgccg cgcgccgctt
     8461 caccggccac gtactcgaag tgggccgtct cgccgcggat cacgcagccg agacagatca
     8521 ccgcgtcgta gcgctcggtc tcggcggcga gctgcgcggc cagcggcagc tcgaaggcac
     8581 ccggcacgga gtacacgtcg ggctcgggtg cgcgatgctc cgccagacag cgtttcgcgc
     8641 cggccaggag ccgttcggtg atctcgtgat ggaactcgga gacgacgatc gcgaaggtga
     8701 actccgacgc gtcgatcggc tcgtcagcgc tgcgcatccg gccggtcagc ggcttctcct
     8761 tccgattggc cgttctcccc gaggtcgagc aggtgcccga gcttcgcctt cttggcgcgg
     8821 aggtaggcga tgttgtgctc ggtgggcttg gtctgcagcg gcacgcgctc ggcgatggtc
     8881 agcccgtacc cttctagccc ggcgcgcttg gtggggttgt tggtcagcag gcgcatagag
     8941 cgtacgccga gatccaccag gatctgcgcg ccgatgccgt aatcgcgcgg gtcgggcgca
     9001 aagccgagcg agacgttggc ctcgacggtg tcctgcccgt cctcttgcag ccggtaggcc
     9061 ttgagcttgt gcagcaggcc gatgccgcgg ccttcgtggc cgcgcagata cacgatcacg
     9121 ccgcggcctt cagaggcgac ctgtttcatc gcatcttcga gctgcgtgcc gcagtcgcag
     9181 cgcagcgagc cgaacacgtc gccggtcagg cactcgctgt gcatgcgcgt gagcacgttc
     9241 tggccgtccc cgatgtcgcc gagcgtgagc gcgatgtggt gccggtcgtc gaccatcgac
     9301 tcgtagccga cgacggtgaa cgtgccgaac ggcgtcggca gcgacgcttc cgcctcgcgg
     9361 cgaaccagcc tttcgatcgt gcggcggtac gcgatcaggt cggcgatcga gatcagcttg
     9421 aggttgtgct cgcgcgcgac ctcgatcagc tccggcaggc gcgccatggt tccgtcgggg
     9481 ttcatgattt cgcagatgac acccgccgga tacatgccgg cgagcaccga cagatccacc
     9541 gccgcctcgg tgtggcccgc gcgcttgagc acgccgcccg gctgcgaccg cagcgggaag
     9601 atgtgacccg gcttgcggaa gtcggccggc tcggcctgcg gatcgagcac cttgagcacg
     9661 gtcgaggcgc ggtcgtgggc gctgatgccg gtgcctccat cggacagatc gatcgagatc
     9721 gcgaacgcgg tctcgttggt gtcggcgttg tcgggcacca tcggcgggat cccgagtgcg
     9781 tcgagccgct cccccaccac cggcatgcag atcaggccgc gtccgtgtgt ggccatgaag
     9841 ttgatccagt cgggcgtgac cttctcggcc gccatgacga agtcgccttc gttctcgcgg
     9901 tcggcgtcgt cgaccacgat caccgcgcgg ccggctttga gctcttcgag tgcctcgtcg
     9961 atcgttgcga acatggatgc tctctttcgg tccgtcagcg gcgtccgctg cgggtcgcgg
    10021 ccggcttctt gatcttggtg ctcgcggcac gggtggtctt cgaggatttg gccggtgccg
    10081 gcctgcgcgc ggcgctgacc gccttggcgg gagccttctt cccgagcgcg ggcctggcct
    10141 tgaccggggg cttcgccggg gcgccggcct tcgcggcggg cttggccttc gcggcgggct
    10201 tggccggctt gacgggcgcc ttcggtttgg cgggcggctt ggacgacacc ttcggcgagg
    10261 gccgggttgc ggccgccggg ccgggggtca ccgcggtcat gccgggcacc gggcccgcgg
    10321 gcggcggcgc ggacgcgctc acggcgggga ccggtccggt ctcgtctccg cgaagcggca
    10381 tcttcaccag ttccgcgagg gacggctgcg ggcgcggcga acgggcggcg atgtgacgct
    10441 ccacgtactt ggagagcacg tccacctcga cgttgaccgt ggagccctgg atcttcttgc
    10501 cgagcgtggt catgccgatc gtgtgcggga taagcgcgac gcggaaccgg tcgcggttga
    10561 cgtcgaccac cgtcaggctg accccgtcca ccgccaccga gcccttcggc gcgagatagc
    10621 gggtcagctc gaacggaacg ttgatctcga gcatctcctg cgcgcccacc ttgcggcgcg
    10681 accggaccac gccgaccacg tcgatatggc cttggacgat gtgcccgtcg aagcgtgcgt
    10741 cggcgcgcat ggaccgctcg atgttgatct cctcgtgtgg ctgcagcatg ccgagcgtgg
    10801 tgcgcgccag cgtctcggga accaggtcgc aggcgaacgc gtcgccttcg atggcggtca
    10861 cggtcacgca ggcgccgttg accgcgaccg agtcgccgat cttgaggccg tcgagcatgc
    10921 cgggagcgcg gacctcgagc cgcgcgcctc ccccgcggcg cagcagccgg atgacccggc
    10981 ccattccttc cacgatcccg gtgaacatag gtggcttccc cctggtgcga cgggcggcta
    11041 aggccgcgga tatgcctcga cgcgcagatc tgcgccggcg tgacggacgg aatgaacgac
    11101 cagctcgcgc gcttccgcga tggtcggcgt gacgaagcct gcgatcgccg gcagccccga
    11161 ggaccccagc agcttcggcg cgagatagaa cacgtaccgg tccacgagtc cgtgctcgac
    11221 cgcgtcgccg agcaccgccg gccctccttc gatcatgacc tccatgacgt cgcgtccgcc
    11281 caggaagtcg agcgtggccg ccaggtcgat gcgaccgtcg cgcgcggggg cgcgccagac
    11341 ctcggcgccg tgcgcgcgaa gggcggcgac cgcgtcctcg ggcgccttgt ccgtcacggc
    11401 cacgagcgtg ggcgcctccg cgttgagcac cgacgattgc agcggcgtcc ggcccgacga
    11461 gtccagaacc acccggagcg gctgtcggcc cttgaatccg cgcaggcgca ccgtaaggcg
    11521 ggggtcgtcc ttgagcacgg tgccgacgcc gaccaggacc gcgtccaccg agccgcgcag
    11581 gcggtgcgcg tcacgccggg ccgtgggtcc ggtgatccac ttcgagctgc cgtccaccgc
    11641 ggccgcgcgg ccgtcgagcg tggcggcgat cttgccggtg acgaaggcgc gtccggtgag
    11701 cacgtgcttg gcgaacgcct cgaccatgcg gcggcagtcc agctcgagca cgcccgcctc
    11761 gacgtcgatg cccgcgtcgt gaagaaagcg aatgcccgcg ccgttcacgc gcgggttggg
    11821 atcggcggtg ccgatcacca cgcgccggat tccggcggcg gcgacagcgg gcgcgcacgg
    11881 cggcgtgcgt ccgtccaccg tgcacggc
//
LOCUS       DATLFO010000312         7460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_136951, whole genome shotgun sequence.
ACCESSION   DATLFO010000312 DATLFO010000000
VERSION     DATLFO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136951"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VM841_12565"
     CDS             complement(<1..408)
                     /locus_tag="VM841_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVL91058.1"
                     /translation="MTPKAAPARRGSVEVSRAAQWYKDAIIYELHVRAFADSDGDGIG
                     DLRGLLGKLEYLSDLGVTAVWLLPLYPSPLRDDGYDIADYTDINPDYGTLDSFREFLA
                     AAHQRGLRVIGELVINHTSDQHRWFQRARRAPPG"
     gene            complement(405..2420)
                     /locus_tag="VM841_12570"
     CDS             complement(405..2420)
                     /locus_tag="VM841_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HVL91059.1"
                     /translation="MGAASPRTGAEPAAPAEADRRARVIIENVDPEIDCGRFPVKRTP
                     GEQVQVEADVFCDGHDVVAAVVRFGRGDDLNEVRMKPLINDRWRAVFSVGDELGAEHY
                     TIQAWVDRFATWQRDLRKRVDANEDVTVPLASGAALIRGAAERASGEDASRLRGIADR
                     VEAGDTRDALDEELAALMLRYDERPHAVAYVRTLPVLIEPERARFSTWYEVFPRSVTD
                     DPNRHGTLRDVTGLVPSIAESGFDVLYLPPIHPIAHTNRKGRNNNPVSEPGDVGSPWA
                     IGAEEGGHTAIHPDLGTFDDFDALVAACRDHGIDLAVDIAFQCSPEHPYVKEHPEWFK
                     WRPDGTIQYAENPPKKYQDIYPLDFECEAWRELWDELASVVRFWVDRGVRIFRVDNPH
                     TKPFRFWEWLIAEIKRDHPEVIFLAEAFTRPRVMERLAKLGFSQSYTYFAWRNTKWEL
                     EQYMNELTTTELRHYFRPNFWPNTPDILTEYLQYGGRGAFMARLVLAATLSSSYGIYG
                     PAFELMEGRAAAHGSEEYLDSEKYQIRIWDRDRPDGLRDLVSRLNRMRHQHPALQTND
                     RFKLHHVDNDNIIGYSKVSADGSNVVLCFVNVDPYHVQAGWTWLDLEELGIDGSRPFQ
                     VHDLITDAHYLWNGPRNYIELSPWIVPAHVFAVRTRVRTEQDFDYYL"
     gene            complement(2515..3108)
                     /locus_tag="VM841_12575"
     CDS             complement(2515..3108)
                     /locus_tag="VM841_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL91060.1"
                     /translation="MRILIITRDYPGKGIDAPSAGDACLALTQTGHEITVVTSEGHAP
                     ADAPVRTVSAGEYPPMLAGIDETAWLLQLNIAMFERAAVELERVPIDVVHAFGSSVAH
                     AAVSLRRVFGVPLVVSMTPGRGDPDAEAWMGRDADRVVVASGRGAGRIEAPPGRVVVV
                     RGGRGYATRLMSVYRDARHAGLSPVTRATPPRKRTNG"
     gene            3187..5316
                     /gene="glgP"
                     /locus_tag="VM841_12580"
     CDS             3187..5316
                     /gene="glgP"
                     /locus_tag="VM841_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HVL91061.1"
                     /translation="MSQIEDRNLRRTVENLASRLPIELRSLAEIAYNFEWSWLPGGRE
                     MFRDIAPLRWSATNENPVQFLIECSGETLDRAVRNGIGERADAIVERIRADCAAPLAH
                     ANADASRPVAFLCTEFAVHRSLPIYAGGLGVLAGDILKEASDMRLPLAGVGLLYRQGY
                     FRQRFDSTGWQHEYWSQLDPELVAATLIRGEDGKPVAITVPIRGRDVVVQIWRVQVGR
                     IPLYLLDAWHPANSAIDRWITSRLYVGDRRYRFAQYALLGIGGVRALRALGLDPLAFH
                     MNEGHAALAPLEIARAEIEAGEPFEAALKKAKSRMLFTTHTPVPAGNEAYTAHEVLEA
                     IGTFPAALGVDDKDLLSLGRANPDDDDEPFGLTPLGLKLSRHANGVSRRHGIVAREMW
                     HHMYPERGVEGVPIGHVTNGVHVATWLAAPMRDLLDKHLGEDWQRRIDDPATWEPVDD
                     IPDEELWDVRLALRANLIEYARERATQDRLSRGEPIEYAEAASTAMDPNALTIGFARR
                     AASYKRLYLLMLDQERALRFLQSNPVQLLIAGKPHPSDEEGKRIVQSVFGLKWAPLVG
                     QRVAYLQDYHLGMASLYVAGSDVWVNLPRPPLEASGTSGMKAAVNGCLNVSVLDGWWE
                     EGCDGTNGWGIASHGSGEAQDIQDATALFDVLENQVLPLFLDRDERGIPRGWVQRIKN
                     SLRTCGPRFSAARMMGEYASLYGLRDV"
     gene            5373..5702
                     /locus_tag="VM841_12585"
     CDS             5373..5702
                     /locus_tag="VM841_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91062.1"
                     /translation="MEIPQDWAEEMEKHDHDAIVHHHPHWHVTHNHNDRTGGFDHLFS
                     GHEHEHDHTALQHTHLPHRNFEEEHQGEAHDHDHGEPVKKRAPAKKAAVKTDAAATPA
                     KRAKKSS"
     gene            complement(5777..6109)
                     /locus_tag="VM841_12590"
     CDS             complement(5777..6109)
                     /locus_tag="VM841_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVL91063.1"
                     /translation="MSPTRRSPSSPNSQPRPNVSGDCGSTRGSWNAGGPPSHPGTVTE
                     YDLMPGGTIAHYMTDPEGQKYPGGMRILRVEPPTLLEFEGYFADDSGAPNPDLPVSRA
                     IALSEKNP"
     gene            complement(6128..6421)
                     /locus_tag="VM841_12595"
     CDS             complement(6128..6421)
                     /locus_tag="VM841_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVL91064.1"
                     /translation="MPRGFDAGGRVSCPAAATSALARNYEMSTTRSRSTSRCLEAAGL
                     VRKDRRGRETIVVPEPAALAAGRTALDRLEDLWRERLERFGRVLADLKQEDHR"
     gene            6420..6896
                     /locus_tag="VM841_12600"
     CDS             6420..6896
                     /locus_tag="VM841_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91065.1"
                     /translation="MRRLIVAVLALGVVLAGAGARGHDGCEISAGAGSHHLPAQPCLH
                     VPFADPACRVGPDRIACLSQIDGTHTQACLYTDAARLAAPWYRGAAAGCAWTAQGGMF
                     FFDPGEDIGAAPPGACVEILREDRQDPLVCLHTEGSRESCLVWTSFFRVCWVPDEE"
     gene            6986..7059
                     /locus_tag="VM841_12605"
     tRNA            6986..7059
                     /locus_tag="VM841_12605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7020..7022,aa:Pro,seq:cgg)
BASE COUNT         1211 a         2464 c         2589 g         1196 t
ORIGIN      
        1 gcccggcggc gcgcggcggg cgcgctggaa ccagcggtgc tgatccgagg tgtggttgat
       61 gaccagctcg ccgatcaccc gcagcccgcg ctggtgcgcc gcggccagga attcgcggaa
      121 tgaatcgagc gtgccgtagt cggggttgat gtcggtgtag tcggcgatgt cgtagccgtc
      181 gtcgcgcagc ggcgagggat acagcggcag cagccacacc gcggtgacgc cgaggtccga
      241 gaggtactcg agtttgccga gcagaccgcg cagatcgccg atcccgtcgc catcactgtc
      301 ggcaaaggcg cgcacgtgaa gctcgtagat gatcgcgtcc ttgtaccact gcgctgcccg
      361 ggacacctcg accgagcccc ggcgggcggg agcggccttc ggcgtcacag gtagtagtcg
      421 aagtcttgtt cggtccgcac gcgcgtgcga acggcgaaca cgtgggcagg cacgatccag
      481 gggctgagct cgatgtagtt gcgcggcccg ttccacaggt agtgcgcgtc ggtgatcaga
      541 tcgtgcacct ggaacggacg gctgccgtcg ataccgagct cctccaggtc cagccatgtc
      601 cagccggcct gcacgtgata cggatccacg ttcacgaagc acagcacgac gttgctgccg
      661 tcggccgaga ccttgctgta gccgatgatg ttgtcgttgt cgacgtggtg gagcttgaag
      721 cgatcgttgg tttgcagcgc cggatgctga tgccgcatcc ggttcaagcg cgagaccagg
      781 tcgcgcaggc cgtcgggccg gtcccggtcc cagatccgga tctggtattt ctccgagtcc
      841 agatattcct cgctgccgtg cgcggccgcg cggccctcca tcagctcgaa cgccggcccg
      901 tagatgccgt agctcgacga cagcgtcgcg gccagcacca gccgcgccat gaacgcgccg
      961 cggccgccgt actgcagata ctcggtgagg atgtcgggcg tgttcggcca gaagttcggc
     1021 cggaagtagt ggcgaagctc ggtggtggtc agctcgttca tgtactgctc gagctcccac
     1081 ttggtgttgc gccacgcgaa gtaggtgtag gactggctga agccgagctt ggcgagccgt
     1141 tccatcaccc gcggccgcgt gaacgcctcg gcgaggaaga tcacctcagg atggtcgcgc
     1201 ttgatctcgg cgatgagcca ctcccagaag cggaacggct tggtatgcgg gttgtccacg
     1261 cggaagatgc gaacgccgcg atcgacccag aagcgcacga cgctcgcgag ctcgtcccac
     1321 agctcgcgcc acgcctcgca ttcgaagtcg agcggataga tgtcctgata cttcttcggt
     1381 gggttctcgg cgtactggat ggtgccgtcc ggccgccact tgaaccattc cggatgctcc
     1441 ttgacatagg gatgctccgg cgagcactgg aacgcgatgt ccacggccag gtcgatgccg
     1501 tgatcgcggc acgcggccac gagcgcatcg aagtcgtcga aggtgccgag gtccggatga
     1561 atcgcggtgt ggccgccctc ctcggcgccg atcgcccagg gcgagccgac atcaccgggc
     1621 tcgctgaccg gattgttgtt gcggcccttg cggttggtgt gcgcgatcgg gtggatcggc
     1681 ggcaggtaga gcacatcgaa gccgctctcg gcgatcgagg gcaccaggcc ggtcacgtcc
     1741 cgcagcgtgc cgtgacggtt cgggtcgtcg gtcaccgagc gcgggaacac ctcgtaccag
     1801 gtgctgaaac gcgcgcgctc gggctcgatc aggacgggca gcgtgcgcac gtaggcgacc
     1861 gcgtgcggac gctcgtcgta gcgaagcatc agcgcggcga gctcctcgtc gagcgcgtcg
     1921 cgggtgtcgc cggcttccac gcgatccgcg atgccgcgga gccgcgaggc gtcctcgccg
     1981 ctcgcgcgtt cggcggcgcc gcggatcaga gccgcgccgc tggcgagcgg cacggtcacg
     2041 tcctcgttcg catccacgcg tttgcgaagg tcacgctgcc aggtcgcgaa gcggtccacc
     2101 cacgcctgga tcgtgtagtg ctcggcgccg agctcgtcgc cgacggagaa gaccgcgcgc
     2161 caccgatcgt tgatcaatgg cttcatgcgg acctcgttca ggtcgtcgcc gcgtccgaag
     2221 cgaaccacgg ccgcgaccac gtcgtggccg tcgcagaaga cgtcggcttc gacctgaacc
     2281 tgctcgccgg gcgtgcgctt gaccgggaat cgtccgcagt cgatctcggg atcgacattt
     2341 tcgatgatga cgcgggcacg ccggtctgcc tcggccggcg cggccggctc cgcaccggtt
     2401 cgcgggctcg ccgctcccac acgggccccg ggcccgccgt tcggtgtttt tcgcgaggaa
     2461 ggcatggaat ctggacccat cctaccccgt ctctcggcag gcgagtccgc cgactcaacc
     2521 attggttcgc ttgcggggcg gcgtcgcgcg cgtcaccggg ctcaagcctg cgtggcgagc
     2581 atcgcgatac acgctcataa gacgtgtcgc gtagccccgg ccgccgcgca cgacgaccac
     2641 acggccgggc ggagcctcga tccggccggc cccgcgaccg gaggcgacca cgactcgatc
     2701 ggcgtcgcgg cccatccacg cttccgcgtc cggatcgccg cggccggggg tcatcgatac
     2761 gaccagcggc acgccgaaca cgcgccgcag cgacaccgct gcgtgcgcca ccgacgagcc
     2821 gaaggcatgc acgacgtcga tcggcacccg ttccagctcg accgcagcgc gctcgaacat
     2881 cgcgatgttg agctgcagca gccatgccgt ctcgtcgatg cccgccagca tcggcgggta
     2941 ttcgccggcg gagacggtgc ggaccggcgc atcggcaggc gcatgccctt ccgaggtcac
     3001 gaccgtgatt tcgtgcccgg tttgtgtcag tgcgaggcat gcatctcccg ctgagggggc
     3061 atcgatgccc ttgccgggat aatctcgtgt gatgatcagg attcgcatag gcagttgggc
     3121 cggcgggcgc ggccacgcat tgtggcatgc gagcaccacg gcgagcaacg catgacggga
     3181 gggtcgatga gccagatcga agaccgcaat cttcgacgca cggtcgaaaa tctggcctca
     3241 cgtcttccga tcgagcttcg ctcgctcgcc gagatcgcct acaacttcga atggtcgtgg
     3301 ctgcccggcg gccgtgaaat gtttcgcgac atcgcgccac tgcgctggtc ggcgaccaac
     3361 gagaacccgg tgcagtttct gatcgaatgc tccggcgaga ccctcgaccg cgcggtgcgc
     3421 aacggcatcg gcgagcgggc cgacgcgatc gtggagcgca tccgcgccga ctgcgccgcc
     3481 cccctcgcac acgccaacgc cgacgcgtcg cgcccggtcg cgttcctgtg caccgagttc
     3541 gccgtgcacc gctcgctccc catctatgcg ggcggcctgg gcgtgctggc cggcgacatc
     3601 ctcaaagagg cttccgacat gcgcctgccg ctggccggag tgggcctgct gtaccggcaa
     3661 ggctatttcc ggcagcggtt cgactcgacc ggctggcagc acgagtattg gtcgcagctc
     3721 gatcccgagc tggtggccgc gacgctgatt cgcggcgagg acggcaaacc tgtcgcgatc
     3781 acggttccca tccgcggccg cgacgtcgtg gtgcagatct ggcgcgtgca ggtgggccgg
     3841 atcccgctgt atctgctgga cgcctggcat ccggcgaact cggcgatcga ccgctggatc
     3901 acctcgcggc tttacgtcgg tgaccgccgc taccgcttcg cgcagtacgc gctgctgggg
     3961 atcggcgggg tgcgggcgct gcgcgcgctc gggctcgatc cgctcgcgtt ccacatgaac
     4021 gaaggtcacg ccgcgctcgc gccgctcgag atcgcccgcg ccgagatcga ggccggcgag
     4081 ccgttcgagg ccgcgctgaa gaaggcgaag tcgcggatgc tgttcaccac gcacacgccc
     4141 gtgcccgccg gaaacgaggc ctacaccgcg cacgaagtgc tcgaggcgat cggcaccttc
     4201 cccgcggcgc tcggcgtgga cgacaaggat ctgctctcgc tcggccgcgc caaccccgac
     4261 gacgacgacg agccgttcgg cttgacgccg ctgggactga agctgagccg ccacgcgaac
     4321 ggcgtgagcc gccggcacgg gatcgtggcg cgcgagatgt ggcatcacat gtatcccgag
     4381 cgcggcgtcg aaggcgtgcc gatcggccat gtcaccaacg gcgtgcacgt ggcgacctgg
     4441 ctggctgcgc cgatgcgcga tctgctcgac aagcatctgg gcgaggactg gcagcgccgc
     4501 atcgacgatc ccgcgacctg ggagccggtc gacgacatcc ccgacgagga gctgtgggac
     4561 gtgcggctcg cgttgcgcgc gaacctgatc gagtacgcgc gtgagcgcgc cacccaggat
     4621 cgcctctcgc gcggcgagcc catcgagtac gcggaagcag cctcgaccgc gatggatccg
     4681 aacgcgctca ccatcggctt cgcgcgccgc gccgcgtcgt acaagcggct gtatctgctg
     4741 atgctcgacc aggagcgtgc gctgcgattc ctgcaatcca atcccgtgca gctgctcatc
     4801 gccggcaagc cacatccgtc ggatgaggaa ggcaagcgga tcgtgcagag cgtgttcggc
     4861 ctgaagtggg cgccgctcgt ggggcagcgc gtcgcgtacc tgcaggacta ccacctcggc
     4921 atggcgtcgc tgtacgtggc cggcagcgac gtgtgggtca acctgccgcg gccgccgctc
     4981 gaggcgagcg gcaccagtgg gatgaaggcc gccgtcaacg gatgcctgaa cgtgagcgtg
     5041 ctcgacggct ggtgggagga aggctgcgat gggaccaacg gctggggcat cgccagccac
     5101 ggcagcggcg aggcccaaga tatccaggac gccactgccc tgttcgacgt gctcgaaaac
     5161 caggttctgc cgctgttcct ggaccgcgac gagcggggca tcccccgagg gtgggtgcag
     5221 cgaatcaaga actcgctgag gacttgtggt ccgaggttca gtgctgcgag gatgatgggc
     5281 gagtatgcga gcctgtacgg cttgcgggac gtttgagcga tcgatccacc ggaaaagaag
     5341 agccgaatcc ggcttgaagg aggagcgacg caatggagat cccgcaggac tgggccgaag
     5401 agatggagaa gcacgatcac gatgcgatcg tgcaccatca cccgcactgg cacgtgacgc
     5461 ataaccacaa tgaccggacc ggcgggttcg atcatctgtt cagcggtcac gagcacgagc
     5521 acgaccacac ggccttgcag cacacccacc tgccgcaccg aaacttcgag gaagagcatc
     5581 agggcgaggc ccacgatcac gaccacggcg agcccgtgaa gaagcgcgca cccgccaaga
     5641 aggccgcggt caagaccgat gcggccgcga cgccggcgaa acgcgcgaag aagtcgtcgt
     5701 aagaagtcgt cgcagcagag gtcagcttcc gaagcgcttg tcgtactggt tcgcacgcgg
     5761 gcgtgatcgg cgggggtcag gggttctttt cggaaagcgc aatcgctctc gagaccggca
     5821 ggtcggggtt gggcgcgccg ctgtcgtcgg cgaagtagcc ctcgaactcc agcagcgtcg
     5881 gcggctcgac gcgaaggatg cgcataccgc ccggatactt ctgtccttcg gggtccgtca
     5941 tgtagtgggc gatggttccg ccgggcatga ggtcgtattc ggtgaccgtc cccggatgcg
     6001 acggcggccc accagcgttc caactgccgc gggtcgaccc acagtcgcca gagacgttcg
     6061 ggcggggctg cgaattgggt gatgacggtg agcgtcgcgt tggcgagatc cttctgaacg
     6121 gtcgcggtca tcggtgatcc tcttgtttca ggtcggcaag gacgcggccg aaacgctcca
     6181 gccgttcgcg ccacaggtct tcgaggcggt ccagagccgt gcggcccgcc gcgagcgccg
     6241 cgggctcggg caccacgatc gtctcgcgcc cccggcggtc cttgcgcacc agacccgcgg
     6301 cctccaagca ccgcgacgtg cttctggatc gcgtggtgct catctcgtag ttgcgagcaa
     6361 gcgccgaggt ggcggcagcg ggacaggaga ccctgccgcc ggcgtcgaac ccgcgtggca
     6421 tgaggcgtct gatagtcgcg gtcctcgccc tcggagtggt cctggccggg gccggagcgc
     6481 gtgggcacga tggctgcgag atctccgccg gagcagggtc ccaccatctg cccgcgcagc
     6541 cgtgtctgca cgtgcccttc gccgaccccg cgtgccgtgt cggcccggat cggatcgcgt
     6601 gcctgtcgca gatcgacgga acgcatacgc aagcgtgcct gtataccgac gccgctcgcc
     6661 tggccgcgcc ctggtatcgc ggggccgccg ccggatgcgc ctggaccgcg cagggcggca
     6721 tgttcttctt tgaccccggc gaggacatcg gtgccgcccc gcccggggca tgcgtcgaaa
     6781 tcctgcgcga ggaccgccag gatccgctcg tgtgcctcca cacggaaggg tcccgggagt
     6841 cgtgcctggt ctggaccagc ttctttcgag tctgctgggt cccggacgag gagtgaaggg
     6901 gcggcccggg tgcgggccga gaccggatgg gcccgtttgc aggacgcgac agcagcacgg
     6961 actcagccgt atactggccc gcctacggga agtagcgcag cttggcagcg cgccacgttc
     7021 gggacgtgga ggccgggggt tcaaatcccc ccttcccgac ttttttgcaa gcctctgacc
     7081 agggaaaacg cgatttttca caatcgccgt gtgcatccgt gtgcatttcc gaacgggaaa
     7141 tcgcgaggta tttcgccgat cgggtgtcca gtccgctgga cgacctgacg cggcatccgg
     7201 aaggaagacg tcgcccgcct tgtggagcgt cgttcgccgc cccgggctcc ccggatgatc
     7261 tccggccgcc tgtcggggag ggaagacgtc cgcgcggcgt gacctatcat gactcggagc
     7321 acgctgggag aacgaagaag gagcctcgtg gggaccaagc agcgggacga ggtcgggatc
     7381 gagatcgtct tcgaaccggc cataacgcgg tcaaagcagc gcgtggccga ccatggggag
     7441 gttttcaccc cggcgtggat
//
LOCUS       DATLFO010000313         9611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_126150, whole genome shotgun sequence.
ACCESSION   DATLFO010000313 DATLFO010000000
VERSION     DATLFO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126150"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VM841_12610"
     CDS             <1..1342
                     /locus_tag="VM841_12610"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVL91066.1"
                     /translation="PYGDGVVTGWGSIEGRRVFVFAQDFTVFGGALGEVFSEKICKVM
                     DLALSTGAPLIGLNDSGGARIQEGVVSLASYGYIFERNVRASGVIPQISVIMGPCAGG
                     AVYSPAMTDFIFMVKDTSHMFITGPDVIKTVTGEEVTFEQLGGAMTHASKSGIAHFAA
                     EDEKHCIQMVRYLMSFLPSNNLESPPFYPPTDPADRREERLVELMPDSSNQAYDMHEV
                     IKLVVDDGEFMEVFPHFARNIITGFARLGGHVVGIVANQPMFLAGVLDVTSSEKAARF
                     VRTCDAFNIPLISFVDVPGFLPGTNQEWEGIIRRGAKLLYAFAESTVPRIQVITRKAY
                     GGAYVVMNSKSIRCDIAYAWPTAEIAVMGAEGAVNIVNRKELEQAEDKATRRAELIEE
                     YTEKFANPYVAAERGYVDDVIDPRDTRPKLIEALEMLRTKRETLPPRKHGNIPL"
     gene            1339..1611
                     /locus_tag="VM841_12615"
     CDS             1339..1611
                     /locus_tag="VM841_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91067.1"
                     /translation="MSDAPRIEITGGAPAPDEEAAVREAILTIWRDDVAAARRGAGES
                     GWLVAARAAGARTRVHETASAHAWTLSLRFDAGPVSVRRTGRGDSK"
     gene            complement(1612..1899)
                     /locus_tag="VM841_12620"
     CDS             complement(1612..1899)
                     /locus_tag="VM841_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91068.1"
                     /translation="MPRKNVPDRVRRRRKAESEAAEERTLDPNVTLGGPPGWTVRTVQ
                     PARATKEYRCPGCNQEIRPGTGHVVAWKEGDDGGRRHWHRGCWDSHRKRLP"
     gene            1942..2586
                     /locus_tag="VM841_12625"
     CDS             1942..2586
                     /locus_tag="VM841_12625"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVL91069.1"
                     /translation="MGEPLIAIVVYPRFTALDAVGPYEVLSRVPGYRTLFVAQTLDLV
                     TTDRGLTIKPDGTFAGVSSPAVLLVPGGPGQIEQMENRALLGYLKRAAASAQMVVSVC
                     TGSLLLGKAGLLEGRRATTHWLALDALAELGAEPVAKRVVRDGNVVTGAGVSAGIDLA
                     LAIAAGLSDEKTAMSIQLGIEYDPKPPFDAGSPAKAPPEIVEAMRARARKYLEA"
     gene            complement(2758..3522)
                     /locus_tag="VM841_12630"
     CDS             complement(2758..3522)
                     /locus_tag="VM841_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL91070.1"
                     /translation="MGLEHFSLEGKTALVTGGSRGIGEAIAIAFAEAGADVAVAARSA
                     DALEAVAGKISATGRRGLPVVCDVTDESQVRACVERVVGELGKIDVLVNNAGGNRFLA
                     PVPDLRPDGFDKIVNQNLRSAFLFSHYVGQHMRERSSGSIINMSSVAGLEGAPGLSAY
                     SAAKFGLRGLTMSMAKELAAAGVRVNAISPGWIRTDLNVNFRGDEQAEKAFVQNVPMQ
                     RWGEAGEIAGAAIFLAGQAASYVTGTTIVVDGGLTA"
     gene            complement(3563..5332)
                     /locus_tag="VM841_12635"
     CDS             complement(3563..5332)
                     /locus_tag="VM841_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HVL91071.1"
                     /translation="MSFEIKTVGIVGCGTMGAGIAEICARNGYTVVFRELDDERVADG
                     IARIEQSIQRGVDKDKFSAGDATAALGRISGGTSWEHLKDADLVIEAVPEHLDLKQEA
                     FKQLDAVLAPEAIIATNTSSLPVIEMAVSTGRPSRVLGFHFFNPAPVMDLVELIKTVV
                     TDEEVVESAKAFAASLGKTPVVAADRAGFIANLLLFPYLNNAVRMLESGFASREDIDA
                     SMMLGTAHPMGPLALVDLIGLDTTYEILDAMYRQFRETLYAPAPLIKQLVTAGFLGRK
                     AGRGFYEYEESGSPRVKESGHHRREADPAAAAHLRKIGVLGTGTMATGIAEVAAKAGY
                     KVVLRGRTQQRVSEATAALDKSLARAVSKGKMTEEQMESTRALVKGTTEMNDFADCDI
                     VIEAIAEDLDVKVEHFKELDEILPANAILASTTSSLPVVALAAATKRPEQVVGMHFFN
                     PAAIMRLVEVVKTVRTSEETADAVLALGKKLGKHCVVCPDRAGFIVNALLFPYLNDAV
                     KMLESGYAKAEDIDAAMKLGCGHPMGPFALMDIVGLDVTYSIVQTLYREFREPGYAPA
                     PLLEHLVHAGYLGRKTKRGFYTY"
     gene            complement(5413..6837)
                     /locus_tag="VM841_12640"
     CDS             complement(5413..6837)
                     /locus_tag="VM841_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91072.1"
                     /translation="MLQWIEPGGSFLFVWMVVFGLGYGMNRAIRRLAPAAGTGAAIVG
                     GILYAINPFAYTRLVHGHVGLLAGMAVFPLVVIALHHVKQHPAKRTAVLLGASVAVAG
                     FVSIHVAGMLVILIPAVVLAIGWPGRRAAAVAGLTTLALSSWWLAQWILADPPPFTGS
                     DLNAFATVPQGFTSAGHTAALYGFWRVGEFALPRDGVWAWPLTFAPIAVAVLWGAVLW
                     FKARRRVAIAMSALAVLGILLAAGTSFPPTAGIFRWLYETVPGFTVFREPQKWVAVVV
                     FAYAILMAPAVDRLIRFASPEPRIAGRVPFERATVVVACLAVIGFYGRTMILNFDVLR
                     PVRFPADWHAVNDTMRESPSRALVFPWHLYISFTWTGNRIANPADTFFDVPVVSSDSI
                     EVGTIRTQSRNPESHAVERILFEEGKRDELPNLLASLCTRWVVLYKAADWESYAWLDS
                     SPALTRAFDGPTIRLYRASIPRCT"
     gene            7189..9264
                     /locus_tag="VM841_12645"
     CDS             7189..9264
                     /locus_tag="VM841_12645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91073.1"
                     /translation="MLRVARSVSETAALPAGERQESVRHVAFFVATVAGANASAYAFH
                     VLMIRLLGTSRYGALAAIVGILTVTAVPLAAVQAVVAKRVANPGGRSEAEVVGSALRF
                     GAAAAISIAVVVTLASPLLSSFLDIGLADTVLALAGLAVPSCLLPVGRGVLQGRRRFP
                     TLGLSIITAMVGRLAFGLAFVVAGLGLRGAILALVIGEFVALAVTFIPLRTDLVGSRS
                     MSEVVREMMLDSASAAGALTAFWTLVSIDVVLARAWLPADVAGVYATAAVVGKTVLYL
                     PNAVALLVFPRLAEQPRGLEARRTVVRSLAVVAGVGVLASVGIAAVPAAAAFVFGSGF
                     YRASGTAALLACSMTAFGAVHILIYYCLAIGKPPVKTLWAAVMVEAALMSAFHSRPAI
                     IAAVSLGVGTALVAVVGSLAARATRVITLPDGELWTPPLSDVELSVVTPVYNGGEQVG
                     PRLAALLATLDRENIRADVILVSDGSTDDTPVLARAVGLDRVRVVHYPENCGKGFALR
                     TGMAMATGRHVAFIDSDGDLNPADLVRFLRLMPMYGADLVVGSKRHPLSEVDYPLFRR
                     MMSWAYHVVVRVLFGVNVSDTQTGIKLVRREALAEVLPRMVEKRFAFDLELIVALRRA
                     GHKRILEAPIQLDFQFTSTISKRAVAGIIRDTLAIWYRRFIIRQYDSHAVPGVEKTGV
                     ADVAALSGE"
     gene            9332..>9611
                     /locus_tag="VM841_12650"
     CDS             9332..>9611
                     /locus_tag="VM841_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91074.1"
                     /translation="MHVALITRYPPRHALDAGRGTAGYSKRLARALVAGGCRVTVLAD
                     LLDGEQPEYEEDGVIVRRVWGSGRHAISPIVEAIGAIRPDIVHLQHELF"
BASE COUNT         1522 a         3189 c         3247 g         1653 t
ORIGIN      
        1 gccctacggc gacggtgtcg ttaccggctg gggctcgatc gaagggcggc gcgtgttcgt
       61 cttcgcacag gacttcaccg tcttcggcgg cgcgctcggc gaggtgttca gcgagaagat
      121 ctgcaaggtc atggacttgg cgctgtccac cggtgcgccg ctgatcgggc tcaacgactc
      181 gggcggcgcg cgcatccagg aaggtgtcgt gtcgctcgcc tcctacggct acatcttcga
      241 gcgcaacgtg cgcgcctcgg gcgtgatccc gcagatcagc gtgatcatgg gtccctgcgc
      301 gggcggcgcc gtgtactcgc cggcgatgac cgacttcatc ttcatggtga aggacacctc
      361 gcacatgttc atcaccgggc cggacgtgat caagaccgtc accggcgagg aggtcacctt
      421 cgagcagctc ggcggcgcga tgacgcacgc gtcgaagtcg ggcatcgcgc atttcgcggc
      481 cgaggacgag aagcactgca tccagatggt ccgctacctg atgtcgttcc tgccgtcgaa
      541 caacctggag tcgccgccgt tctatccgcc gaccgatccc gcggatcgcc gcgaggagcg
      601 gctcgtcgag cttatgcccg acagctcgaa ccaggcctac gacatgcacg aagtcatcaa
      661 gctcgtcgtc gacgacggcg agttcatgga ggtcttcccg cacttcgcgc gcaacatcat
      721 caccgggttc gcgcgcctcg gcgggcacgt cgtcgggatc gtggccaacc agccgatgtt
      781 tctcgccggc gtgctcgacg tgacctcgtc cgagaaggcc gcgcgcttcg tgcgcacctg
      841 cgacgcgttc aacattccgc tgatctcgtt cgtggacgtg ccgggtttcc tgccgggcac
      901 caaccaagag tgggaaggca tcatccgccg cggcgcgaag ctgctctacg ccttcgccga
      961 atccacggtg ccgcgcatcc aggtgatcac gcgcaaggcc tacggcggcg cctacgtcgt
     1021 catgaactcg aagtcgatcc gatgcgacat cgcctacgcc tggccgaccg ccgagatcgc
     1081 ggtgatgggc gccgagggcg ccgtcaacat cgtcaaccgc aaggagctcg agcaggccga
     1141 agacaaggcc acccgccgtg cggagctgat cgaggagtac accgaaaagt tcgcgaatcc
     1201 gtatgtggcg gccgagcgag gctacgtgga cgacgtgatc gaccctcggg acacgcggcc
     1261 gaagctcatc gaggcgctcg agatgctccg gaccaagcgc gagaccctgc cgccccgcaa
     1321 gcacgggaac atcccgctgt gagcgacgcg ccgcgcatcg agatcaccgg tggcgctccg
     1381 gcgcccgacg aggaggcggc cgttcgcgag gccatcctca cgatctggcg cgacgacgtc
     1441 gcggccgcgc ggcgcggcgc cggcgagagc ggctggctgg tcgcggcacg cgcggcgggt
     1501 gcgcgcaccc gcgttcacga gaccgcctcg gcccacgcct ggacgctgtc cctgcggttc
     1561 gacgcgggtc cggtcagcgt gcgccgaacc ggccgcggcg actcgaagta gtcagggcag
     1621 ccgcttgcgg tgggagtccc agcagccgcg gtgccagtgg cggcggcccc cgtcgtcgcc
     1681 ttctttccac gccacgacgt ggccggtgcc gggccggatc tcctggttgc agccggggca
     1741 gcggtattcc ttggtcgcgc gcgcgggctg cacggtccgg accgtccagc cgggcgggcc
     1801 gccgagcgtg acgttcggat cgagcgtgcg ctcctcggcc gcctcggact cggcctttcg
     1861 gcggcgccgc acgcggtcgg gaacgttctt gcggggcatg gaatcgcatc gtaccggcgc
     1921 gcccgttacc atgcgccggt catgggcgag cctctcatcg cgatcgtcgt ctatccgcgg
     1981 ttcacggctc tggatgccgt cggcccctac gaggttctgt cgcgcgtccc cggataccgg
     2041 acgctgttcg tcgcgcagac gctggatctg gtcaccaccg atcgcggcct gaccatcaag
     2101 ccggacggaa cgttcgccgg cgtgtcgtcg cccgcggtgc tgttggtgcc gggcggtccc
     2161 ggtcagatcg agcagatgga gaaccgtgcg ctgctcggct acctcaagcg cgcggccgcg
     2221 tccgcgcaga tggtcgtgag cgtatgcacg ggctcgctgc tgctgggcaa ggcgggactg
     2281 ctggaaggcc ggcgggccac gacgcattgg ctcgcgctcg atgcgctcgc cgagctgggc
     2341 gccgagccgg tggccaagcg cgtcgtgcgc gacggcaacg tcgtgaccgg ggcgggcgtg
     2401 agcgccggca tcgatctcgc gctcgcgatc gcggcgggcc tgagcgacga gaagaccgcg
     2461 atgagcatcc agctcggcat cgagtacgac ccgaagccgc ccttcgacgc cggctcgccc
     2521 gcgaaggcgc ctcccgagat cgtcgaagcg atgcgcgccc gcgcgcgcaa atacctcgag
     2581 gcgtagtccg gctccgtctc caaaccgttc gaagccacgt accggcagtt gatgtcgtga
     2641 atcgagacgg agatcatcga aagcgccgca tcggccgtcg ccgagccgtc gccgagccgt
     2701 gcggtcgcag gggcttcgac gccgtggctt ccgtttcctg ccgcgatttg cgggacgtca
     2761 ggccgtcagg ccgccgtcaa cgacgatggt ggtgccggtg acgtagctcg cggcctggcc
     2821 ggcgaggaag atcgcggcgc ccgcgatctc gcccgcctcg ccccagcgct gcatcggcac
     2881 gttctgaacg aaggccttct cggcctgctc gtcgccgcgg aagttgacgt tgaggtcggt
     2941 gcggatccag ccgggcgaga tcgcgttgac gcgcacgccg gccgcggcga gctccttggc
     3001 catcgacatg gtcaggccgc gcaggccgaa cttcgccgcg ctgtaggccg acaggccggg
     3061 agcgccttcg aggcccgcga cgctcgacat gttgatgatc gagccgctgc tccgctcgcg
     3121 catgtgctgt ccgacgtagt ggctgaacag gaacgcgctg cgaagattct ggttgacgat
     3181 cttatcgaag ccgtcgggcc gcaggtcggg gactggagcc aggaagcggt taccgcccgc
     3241 gttgttgacc agcacgtcga tctttccgag ctcgccgacg acgcgttcga cgcatgcacg
     3301 aacctgcgac tcgtccgtga cgtcgcagac gacgggcagt cctcgccggc cggtcgcact
     3361 gatcttgccc gcgacggcct cgagtgcgtc ggcgctgcgc gccgcgaccg cgacgtccgc
     3421 tccggcctcg gcgaacgcga tcgcgatcgc ctcgccgatc ccccgcgacc cgccggtcac
     3481 cagtgcggtt ttgccttcga gcgagaagtg ctccagtccc atgggtgccc ccttcggctg
     3541 tctcggaccc tcggcccggg acttagtacg tgtagaagcc tcgcttggtc ttgcggccga
     3601 ggtagccggc gtgcacgagg tgctcgagca gcggggcggg tgcgtagccg ggctcgcgga
     3661 actcgcgata cagcgtctgg acgatggagt aggtcacgtc gaggccgacg atgtccatga
     3721 gcgcgaacgg acccatcggg tggccgcagc cgagcttcat cgcggcatcg atgtcctccg
     3781 ccttcgcgta gccggactcg agcatcttga ccgcgtcgtt gaggtagggg aacagcagcg
     3841 cgttgacgat gaagccggcg cggtccgggc acacgacgca gtgctttccg agcttcttgc
     3901 cgagcgcgag caccgcgtct gcggtctctt cgctggtgcg cacggtcttg accacctcga
     3961 ccaggcgcat gatcgcggcc gggttgaaaa agtgcatgcc gacgacctgt tcgggccgct
     4021 tcgtcgcggc cgcgagcgca acgaccggaa gcgatgaggt cgtggacgcc aggatcgcgt
     4081 tggccggcag gatctcgtcg agctctttga agtgctcgac tttgacgtcg aggtcctcgg
     4141 caatcgcttc gatgacgatg tcgcagtccg cgaagtcgtt catctcggtc gtgcccttga
     4201 cgagcgcgcg cgtggactcc atctgctctt cggtcatctt gcccttcgac acggcgcggg
     4261 ccagcgactt gtccagcgcc gccgtggcct cgctgacgcg ctgttgtgtg cggccacgga
     4321 gcacgacctt gtagccggcc ttggcggcga cctcggcgat gccggtcgcc atcgtgccgg
     4381 tgcccagcac gccgatcttg cggaggtgcg cggccgcggc cgggtcggct tcgcggcggt
     4441 ggtggcccga ctccttcacg cgcggcgagc ccgactcttc gtattcgtag aagccacgtc
     4501 cggcctttcg gccgaggaag ccggcggtga cgagctgctt gatcagcggc gcgggcgcgt
     4561 agagcgtctc gcggaactgc cggtacatcg catcgaggat ctcgtaggtg gtgtccaggc
     4621 cgatcaggtc cacgagcgcg agcggaccca tcgggtgcgc ggtgcccagc atcatcgacg
     4681 cgtcgatgtc ctcacgcgag gcgaatcccg actcgagcat gcggaccgcg ttgttgaggt
     4741 acgggaagag gagaaggttc gcgataaagc ccgcgcggtc ggcggcgacc accggcgtct
     4801 tgcccaggct cgccgcgaat gccttcgccg actcgacgac ctcctcgtcg gtgacgacgg
     4861 tcttgatcag ctcgaccaaa tccatcaccg gcgcagggtt gaaaaagtga aagccgagca
     4921 cgcgcgaggg ccggccggtc gagaccgcca tctcgatcac gggaagcgac gaggtgttcg
     4981 tcgcgatgat cgcctcgggc gccaggaccg cgtcgagctg cttgaaggcc tcttgcttga
     5041 ggtccaggtg ctcgggaacg gcctcgatca ccagatcggc gtccttgaga tgctcccagg
     5101 aggtgccgcc cgagatgcgg ccgagcgcgg ccgtggcgtc gccggcgctg aacttgtcct
     5161 tgtccacgcc gcgctggatc gactgctcga tgcgcgcgat gccgtccgcg acgcgttcgt
     5221 cgtcgagctc gcgaaacacc acggtgtagc cgttgcgcgc gcagatctcg gcgatgccgg
     5281 cccccatcgt gccgcatccg acgattccga ccgtcttgat ctcgaagctc atcgtgcctg
     5341 gctccctcgt tggctggatt agccgctagt ttcgcacccg ttgcgcgccg cgaccaaacc
     5401 ggccgcgcgg gctcaggtgc agcgtgggat cgaagcccgg taaagccgga tcgtcggccc
     5461 gtcgaacgcc cgggtgagcg caggagagga gtccagccag gcgtaactct cccagtcggc
     5521 ggctttgtag agcaccaccc accgcgtgca aagcgaggca agaagattcg ggagctcgtc
     5581 gcgtttgcct tcctcgaaca ggatccgctc caccgcgtgc gattccgggt tgcgcgactg
     5641 ggttcggatc gtgccgacct cgatcgagtc gctggagacg accggaacgt cgaagaaggt
     5701 gtccgccgga ttcgcgatgc gattgcccgt ccaggtgaac gagatgtaga ggtgccacgg
     5761 gaagacgagt gcgcgtgagg gactctcgcg catcgtgtcg ttcaccgcat gccagtccgc
     5821 cgggaagcga accggcctca gcacgtcgaa gttcaagatc atcgtgcgcc cgtagaaccc
     5881 gatcaccgcc aggcacgcca cgacaaccgt ggcccgctcg aacgggaccc ggccggctat
     5941 gcgtggctcg ggcgatgcga accggatcag ccggtccacg gccggggcca tcaaaattgc
     6001 gtaggcgaac accaccaccg cgacccactt ctgaggctcc cgaaagacgg tgaagcccgg
     6061 cacggtctcg tagagccacc gaaagatgcc ggccgtcggc ggaaacgacg tgcctgccgc
     6121 gagcaggatg ccgaggaccg cgagggccga catcgcgatg gcgacccgac gccgggcctt
     6181 gaaccacagc acggctcccc aaagaaccgc cacggcaatc ggagcgaacg tcagcggcca
     6241 cgcccaaacc ccgtcgcggg ggagcgcaaa ttctcctacc cgccagaacc cgtagagggc
     6301 ggccgtgtgc ccggccgacg taaagccctg cggcacggtc gcgaacgcgt tcaggtcact
     6361 gcccgtgaag ggtggagggt ctgcaaggat ccactgtgcg agccaccatg aggacaacgc
     6421 gagcgtggta agtccggcga cggctgccgc ccggcgcccc ggccacccaa tcgccagaac
     6481 aacggcaggg atcaggatca cgagcatgcc ggccacgtgg atgctgacga acccggcgac
     6541 ggcaaccgac gctccgagca ggacggcagt tcgtttcgcg gggtgttgtt tcacatgatg
     6601 gagcgcaatc acgacgagcg ggaacaccgc catgccggcg agcaagccga cgtggccatg
     6661 cacgagcctc gtgtaagcaa acggattgat cgcgtagagg atccctccca cgattgccgc
     6721 gcccgttccc gccgcggggg cgagtcgacg aatcgcacgg ttcatcccgt agccgaggcc
     6781 gaacaccacc atccatacga acaggaagct cccgcccggc tctatccatt gcagcaacca
     6841 taggaaagct tgaaacgggg cttgattgac ggggccttgg atgtcggcgg cttgctgcca
     6901 gcggaccgtg acgttcgggc cgtagacgaa atccagaaga agcagatatc cgggccgtag
     6961 taacggtgcc gacacgagaa gagcggcggt ggcgtggacc gcaaccgagt ttctgtcgga
     7021 tcggaagcgg cgaacgatcc gagtcaccgc tgtgggtccg gcccctcgaa tctcgccgcc
     7081 ggatggcgcg aatacgtctg agggaacgcc ggaaactttt gacataacaa ggaggaagcc
     7141 gggagaatct ggcacaatgt accaaatccg gtccggtagg gggagttctt gctccgcgtt
     7201 gcgcgttcgg tgagcgaaac agccgcactg ccggcaggcg aacggcaaga gtcggtacgt
     7261 catgtcgcct tcttcgtggc gacggtagcc ggagccaacg cgtctgccta cgccttccat
     7321 gtgttgatga tccgactgtt gggcacgagc cgctacggtg cgctggccgc gatcgtcggg
     7381 atcttgaccg taacggccgt cccgctggcc gcggttcagg ccgtggtggc caagcgggtt
     7441 gcgaaccccg gcggccgctc ggaggccgaa gtggtggggt ccgcgcttcg gttcggagcg
     7501 gcggccgcga tctcgatcgc ggtcgtggtt accctcgcgt cgccgctgct ttcatcgttt
     7561 ctcgacatcg gtcttgcaga cacggtgctc gctctcgcag ggttggccgt gccgtcgtgt
     7621 cttctcccgg tcggacgtgg ggtcctgcaa ggacgccgcc ggttcccgac actcggcctg
     7681 tcgatcatca cggcaatggt cggccgcctt gccttcggac tcgctttcgt cgtcgccggg
     7741 ctcggactcc gcggggccat tctggccctc gtgatcggag aattcgtcgc ccttgccgtg
     7801 accttcattc cactccgcac ggacctggtc ggatctcgct ccatgtccga agtcgttcgg
     7861 gaaatgatgc tcgatagcgc atcggctgcc ggcgcgctca cggctttctg gacgctggta
     7921 tcgatcgacg tcgtccttgc ccgcgcgtgg ttgcctgcgg acgtcgccgg cgtctacgcc
     7981 actgcggcgg tcgtggggaa gacggtgctg tacctcccga acgcggtggc cttgctcgtt
     8041 ttcccccgcc ttgccgaaca gcctcgaggg ctcgaggcgc ggcgcacggt cgttcgctcc
     8101 ctcgcagtcg tggcgggcgt gggggtgctc gcctccgtcg ggatcgcggc cgtcccggcc
     8161 gcggccgcct tcgtcttcgg atccggcttc taccgtgcat cgggaacggc tgcgctactg
     8221 gcctgctcga tgaccgcatt cggcgcagtc cacatcctga tctactactg cttggccatc
     8281 ggcaaacctc cggtgaagac actttgggct gccgtcatgg tagaggccgc cctgatgtcg
     8341 gcattccact cgaggcccgc gatcatcgcc gcggtatcgc tgggtgtggg aacggcgctc
     8401 gtcgcggtcg tcggttcgct ggctgctcgt gcaacccgcg tcatcacctt gccggacggc
     8461 gagctttgga cacctccgtt gtcggacgtg gaactatccg tcgtgacacc cgtatacaac
     8521 gggggcgagc aggtcggtcc ccggcttgca gcactcctgg cgactctgga tcgcgagaac
     8581 atccgggccg acgtcattct cgtctccgac ggcagcacgg acgacacgcc cgtcctcgct
     8641 cgcgccgttg gcctcgatcg agtgcgcgtc gttcattatc ccgagaactg cgggaagggt
     8701 tttgctcttc gaaccgggat ggcgatggca accgggaggc atgtcgcttt catcgattcg
     8761 gacggcgacc tgaatcccgc cgacctcgtg cgcttcttgc gcctcatgcc catgtacggc
     8821 gccgatctcg tcgtggggtc gaagcggcac ccgttgtccg aggtcgacta ccccctgttc
     8881 cggcgaatga tgagctgggc ctatcacgtg gtcgtccggg ttctcttcgg agtgaacgtg
     8941 tccgatacgc aaacgggcat caagcttgtc cgtcgcgagg cgctcgcgga agttcttccc
     9001 cgaatggtcg agaagcggtt tgctttcgac ttggaactga tcgtggcctt gcgcagggcc
     9061 ggccacaagc gcatcctcga ggcgccgatt cagctcgact tccaattcac cagcacgatc
     9121 tccaaacgtg cggtcgctgg aatcatccgc gatactctcg ccatctggta tcgccggttc
     9181 atcatccgcc agtacgattc gcatgcggtg ccgggcgttg aaaagaccgg ggttgccgac
     9241 gtcgcggcgc tcagcggcga gtgaatctac tgcgctcgat cgcctgacgc cgtcctgttc
     9301 ggccacaccg gggagattca gtgaacaaac gatgcacgta gcgctgatta cgaggtaccc
     9361 accgaggcat gcgctcgatg ccgggagggg gacggctggt tactccaagc ggctggcgcg
     9421 tgctctggtc gccggcggct gccgcgtcac cgtcctcgct gatctcctgg acggcgagca
     9481 gcccgagtac gaagaggacg gggtcatcgt gcggcgtgtg tggggatcgg ggcgtcatgc
     9541 gatctcgccg atcgtcgagg ccattggggc catccgcccg gacatcgttc atcttcaaca
     9601 cgaactgttt c
//
LOCUS       DATLFO010000314        20858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7584, whole genome shotgun sequence.
ACCESSION   DATLFO010000314 DATLFO010000000
VERSION     DATLFO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7584"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..441)
                     /locus_tag="VM841_12655"
     CDS             complement(28..441)
                     /locus_tag="VM841_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91075.1"
                     /translation="MIRRVCIVLALSSLLAMLPAQAAEPPRFLIVAARFMYVPATDVY
                     LSPPTRTARVEMRLPPGVAVALLNTDGDEHDIVSGDPDEHYPYGDGFFESARTRRGEA
                     PVPVAGVENLAPGRYPFFCSVHFGVPSGVLEIVAP"
     gene            619..1986
                     /locus_tag="VM841_12660"
     CDS             619..1986
                     /locus_tag="VM841_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVL91076.1"
                     /translation="MKRRSGMFVALAAVIGSLLPAAASPPVVLPMIQPLHWDGSALRD
                     PAGREVTLRGLEVAAAESAPDADRIASWGFNLVRLGLTWREVEPARGRYDRTYLAAVK
                     SIVHTLASRGIYTLIHFRQDLYHERYGGEGAPDWAVGSPLPPVDTGGGPAGYLTPAVT
                     NEYDLFWGNASGVRDAYADAWMRVAAELSGEPGVLGYDLFGAPWPGSQWPSCTSSAGC
                     PAFDDVILDRFYRQVLGGIRTIDADAMIYVSPAIPASLGAGTWVGPVDDPSASTGLSF
                     RVTCPARPPDHGPAGSGGACGPIESRAMRVQSGRARAVGAHPMLSGVAANGDPASAER
                     LFDLAEVHGVYSFAYDAGGEASLAADHATLKVVARPYAQSVAGTLFEQDWMPDEMLRT
                     LRLVYRANGGDTVVVWPRAIQGEYGAGVVCNPPGHCSVTAAGDRIVVHAAAGAHVIFS
                     ASRLD"
     gene            complement(1990..2694)
                     /locus_tag="VM841_12665"
     CDS             complement(1990..2694)
                     /locus_tag="VM841_12665"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HVL91077.1"
                     /translation="MELGTILGVWAHPDDETYLSAGLMAAGRREGRRIVCVTATRGEG
                     GSWDEDRWPSSELGAIREKELDRCLAVLGVDEHSWLDYPDGGLHEIDDAEGIARVAAI
                     VEEVEPDTILTFGPDGMTGHTDHQAVSRWATAAFARSAKPGATLHYATTVQSWADRWL
                     PVFERFNVFFAGGPPIDKEEDLSINFVLPQDLLDLKLKAIEEHVSQVEGMLEAFGEDV
                     FSESMNAEYFRLAAFK"
     gene            complement(2721..3773)
                     /locus_tag="VM841_12670"
     CDS             complement(2721..3773)
                     /locus_tag="VM841_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91078.1"
                     /translation="MIADAVRARTLIDKASTGLTAAASLEDVLAEAGEALVYATASGD
                     SRLVLDALLLRAQALTAIGDATAEDWLAVERAARDLEDWLRVAFALRMQVMGLLDDHA
                     PDAAAPAARLGDVAAAQAMPRDIAWAEAVRAEIAMVAGRWDVAHTTAMRAVTNANRAE
                     DPTTMLRAWHVVIPIAAARRDEVRLSLAFYAYERRRLTASPYARLLDAAATAQFAAFG
                     LTSAPPLRFEDLPGAFDGAAAMPHWLAAVETIVGGWIEAGEFAAAERALEKMGGGRPT
                     GLGAGVEALLRARLHAAEGSPPQGVAAQAWHALEVFSRIGAPWWIAKAIGALGRNATP
                     TLKKQAKQIELVLRVR"
     gene            complement(3736..4611)
                     /locus_tag="VM841_12675"
     CDS             complement(3736..4611)
                     /locus_tag="VM841_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVL91079.1"
                     /translation="MSDLDLAKYLSASRKLDLEGIDFRRAADFPVSREEIRVLTYMMD
                     IETHTVVYLRDLLASQAAYDPDITAFLSLWVYEELWHGEALAAFLRAVGERHAPARIT
                     SIRCETDRGGAVARWLKTLAARRLPSFVAVHMAWGAINELSTLHGYKRLIATTRNPVL
                     RELLTRIVKDERRHYAFYRAKARVLLKDNPQAQRLVRRVLARFWAPVGTGVRPQAETD
                     FAVAHLFGDAEGTAVLAAMDDEIEQIDGLAGLQLLTRARAGALRRASVPSIEAPSPVS
                     SFKNDRRRRARTHAY"
     gene            complement(4675..5412)
                     /locus_tag="VM841_12680"
     CDS             complement(4675..5412)
                     /locus_tag="VM841_12680"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVL91080.1"
                     /translation="MTTKHHEIEETIAAYALDSMSETERAIILPDLLDHLSACDDCRG
                     VYHDFRATAGDLALGVPAEPVTPAFEAKVMGAIRGEARPVAAKPSRSRWAVRAMLAAS
                     LAAVAALGLFSYSVVGDLREERARIEQSERVLAVLSDPTLQRFSLTSPHGQGAVTLAV
                     ANDGRAVLTGSGLPSIPDDRVFELWLMQNGTPVAAGVFTASGGDAILLLDHDPRRFDA
                     AAITVEPGPAGTQAPTSDVIYSVKLRA"
     gene            complement(5409..6023)
                     /locus_tag="VM841_12685"
     CDS             complement(5409..6023)
                     /locus_tag="VM841_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91081.1"
                     /translation="MSTDRSASDEVGDLQQQRDLASIGKIARGDEHAFGDLFRRYAPG
                     ALGIATRVLVDRVLAEEVVQEVFVSLWKRAGQYDGSRGTVRSWLLAQAHHRAVDVVRR
                     EEAERRRTTTTYAPEPPLPSADDVIEDAWLADRRARVQAALPALPDEQRAVLEKTYFE
                     GLTQAQAAEALGVPLGTVKSRTVAAMRRLRDTLERRSHGGGEQS"
     gene            complement(6103..6498)
                     /locus_tag="VM841_12690"
     CDS             complement(6103..6498)
                     /locus_tag="VM841_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVL91082.1"
                     /translation="MKRPLLLSASAAALALVLAACGGTGNGAAGDPDAPAASASTQDR
                     VEIRMFTFSPGSITVKAGTSVTWVNQDEILHTVTAGSGPDDVTGDYDGQLGDAGSTFS
                     FTYGEAGTFEYFCTRHPGVPGMHGTVVVT"
     gene            complement(6495..8024)
                     /locus_tag="VM841_12695"
     CDS             complement(6495..8024)
                     /locus_tag="VM841_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91083.1"
                     /translation="MTQWFAAVTGVALAAAAGVHLAVLPGHAAESAVHGLFFAATALA
                     QAALAVAVVRRPGRGVLAAVALHSLAITGVWVVSRTIGLPFGLGVEPAGIADSLTAGF
                     QLIAAAGAFALLRRPQRLLVRRGRPSVAIAAVAAATLLSAGIATAAPIASGHRHAGAD
                     HAAAGHAAHGHHDGHLDEHVRHGLPSVEGAHLHGLRAPGDFDPGRDPTRSASSPIDGV
                     AIAVGRQPVDVAEGAGSLWIANRGDATVSRVDPRTRRTVATIPVARQPAGIAYGFGAV
                     WIASHGDDVVQRIDPATNVVTASVRVGTGPSAVVALPTGLWVATVTEGQVQRIDPATM
                     AASAPIATGYGPVALAGDEHSVWVSNALDRTIVRIDAATRRAGTPVPVPAGASGLTID
                     GGGVWVTGATAGTLTRVDTATGRAAPAISVDDRSLPGLGPVAVTAAAGRLWVANNHDK
                     TIVSVDPASGSTGRILFFDNALASHPGQVRLVEAAGALWVTDFERDMIVRVPIPKGTS
                     S"
     gene            complement(8128..9363)
                     /locus_tag="VM841_12700"
     CDS             complement(8128..9363)
                     /locus_tag="VM841_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91084.1"
                     /translation="MKRKSLVIAAGALTLTLAGAACGSTADTVAKPATSVLPGTATRD
                     AGPGSAASGLRATLTDLLVEHEYLAGITISTAVSTGLDSDATKGASEALDKNSVALSE
                     AIDSVYDGAGKSFLPLWRKHIGFFVDYTVGKVSKDEAKQKTAREGLDGYRSDFGAFLA
                     SANPNLTKEAVADALVPHVNSTFDAIDALVAGSPDAFAKLREGAAVMPGIAEVLAGAI
                     AKQFPDKIAGDPGSAGSGLRSALTTLLVEHEYLAGITISTAVANGLDAAPTKAASAAL
                     DANSVALSDAIDSVYEGAGKSFLPLWRKHIGFFVDYTVGKLSNDEAKQKTAREGLDGY
                     RNDFGAFLASANPNLTKDAVADALVPHVNTTFDAIDAMTAKSPTAFVKLQEGAQVMPG
                     IAEVLAGAIAAQFPDKFAK"
     gene            complement(9577..11568)
                     /locus_tag="VM841_12705"
     CDS             complement(9577..11568)
                     /locus_tag="VM841_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HVL91085.1"
                     /translation="MDRIFQGLNEQQAAAVAAVRGPVCILAGAGSGKTTTITRRIANQ
                     IATGTFDASEILAVTFTDKAAREMAARLQRLGVPAVRARTFHAQALALYKAFSSDAAE
                     IVPSKGQILSPLVKRLPPPHKFTAVRDIATEIEWAKNRMIGPDRYIEVATAESRVTPV
                     PVEVMGGLYKTYERRKEAAGLMDFEDLLARTIELLRTDERAMNAVRTKYRAFTVDEYQ
                     DVNLLQQSLLDVWVGERRDLCVVGDDYQSIFGFTGATPRYLLDFPERHDAQVFRLTVN
                     YRSTPQVLNVANRLVPKMGGSPKSLTARAGADGPEPVFREHATGSGEVEWIVRQCRAL
                     RNAGVPWEEMAVLYRINGRSEDLEEAFSADRLPYQVRDGSFLRRPAARSVIAKLKRAP
                     DTAVGPLMDQIARGLGWREEVPDDTDASEATRQADLGRLRALAHEFDDATKPDDAKPV
                     KTRAELFVEDLGKRFAVEREGRGVQILTYHRSKGLEFEAVFLPRMEEKELPFALSKSD
                     AEIAEERRLLYVGITRAKKYLFVSWAAQRPGDRRRSPKRSPFLAEMRPQAVPTSGRLT
                     GTPAGAAAASREAESRPKVAVGDEHLALFTALKDWRLQAARKNGVPAYVVFHDQTLAR
                     IAQERPANVIELGRISGIGPTKIQRYGSEVLDLIGTHAG"
     gene            11837..12619
                     /locus_tag="VM841_12710"
     CDS             11837..12619
                     /locus_tag="VM841_12710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVL91086.1"
                     /translation="MTKAAFFDLDKTLVPGSSLFLLARGMYQRDYYRVRDMAGFAWNQ
                     VMFRLVGSEREQGMEQSKKAALEFVKGKHRDEFAALGNEIVEEQILPRVYEDIARVIR
                     SHTDAGFETFLVTAAPIELARVVSESLGMTGALGTESEFDDAGRYTGRLVGDILHGPE
                     KAKAVQLLALERGIDLSESHAYSDSTNDLPLLETVGHPHAVNPDHGLRRIARTRGWPI
                     HELRARRRALLIGIPSAVAATGLFAGGVALGAWLANRRRTLS"
     gene            complement(12631..13110)
                     /locus_tag="VM841_12715"
     CDS             complement(12631..13110)
                     /locus_tag="VM841_12715"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HVL91087.1"
                     /translation="MPPQGASRIDSPSRYRVLYFAEDPAIAVGEAFASRPVWMEEMFE
                     FPVLPDARSSLITVQVDARVVDLDDAAALAERALRPSIVATPDRTVTQAWAARIFAEK
                     RWAGIRWWSARDARWGVVGLWSLESIRIQRVEPLARDHDAVRRATALLKKRWAKKST"
     gene            complement(13200..13598)
                     /locus_tag="VM841_12720"
     CDS             complement(13200..13598)
                     /locus_tag="VM841_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91088.1"
                     /translation="MGRPGRPKGSGPVRVGEVAPVYGGVGGALSRLEALIAMLGNNRV
                     ARLLDVSASQTSRWRSSKDRPSPDMILRIGDLDHVMFRLFGQMHATVALDWLDAHNEF
                     IGTTPAEAIEAGEFGLVHSALALEAQGGFL"
     gene            13679..14428
                     /locus_tag="VM841_12725"
     CDS             13679..14428
                     /locus_tag="VM841_12725"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HVL91089.1"
                     /translation="MAALNDVREWLREARAVTVLTGAGISTESGIPDFRGPNGLWTRD
                     PEAEKLSDIGWYTSSREIRVKAWKARLQHPAWTAKPNAGHHALVALERAGRMHTLVTQ
                     NVDGLHQTAGTGPERIVEIHGTVREVECLSCQDRAPMEVALERVRGGEDDPACKRCGG
                     ILKSATISFGQSLRPDDLARAERAARECDLFLAVGTSLTVYPVAALPEVAMAHGARLV
                     IVNAEPTPYDPMAHAVLREPIGTILPALVGT"
     gene            14425..15120
                     /locus_tag="VM841_12730"
     CDS             14425..15120
                     /locus_tag="VM841_12730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91090.1"
                     /translation="MSIQVRPLSSLDEIAAADAVFDQVWHAPDRERLPRELLRAMTAH
                     TNLLIGAFDGDTCVGVVTGWWGRDDDAAVWLWSARLGVLESHRRAGVATTLKREQARV
                     ALERGVNEIRWTFDPMRARNAAFNMRTLGARAIAYLDDVYGPRQDRFNAGERTDRLLV
                     SWTPHRPAPAGNPPLTRVRVPAGIDEYLSWPMDRRRDERDRVRRQMRSAFEAGLVVTG
                     FEPPGTYLFGGIA"
     gene            15117..16238
                     /gene="menC"
                     /locus_tag="VM841_12735"
     CDS             15117..16238
                     /gene="menC"
                     /locus_tag="VM841_12735"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HVL91091.1"
                     /translation="MITLRAAELVRADLPLVRPFRTSFGVQELREAVLVRVVCDVEGV
                     EAEGWGECVAGSDPFYSAEWGLGAWVLLREVLLPMLAEEPLTTIEDTPAAMSEVRGNR
                     MAKAAAEMAITDAALRARGVSLKAYLGGERDRVECGVSIGIMDSPDDLVAAVGTYLGD
                     GYTRIKCKIEPEADVAFIAAVRETFPDIALTVDANAAYTLADADLLARLDAFDLDYIE
                     QPLAEEELMEHAALARLIETPICLDETIVSAKSAEDAIALGACEVINIKQGRVGGLAE
                     ASWTHDVCVEHEIPVWCGGMLETGLGRAANLALASLPGFTMPGDTSPSSRYFHEDITA
                     PFEMAPDGTIAVPTGPGLGVAPLPGIMARVVAEREKVEL"
     gene            16235..17296
                     /locus_tag="VM841_12740"
     CDS             16235..17296
                     /locus_tag="VM841_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HVL91092.1"
                     /translation="MRDMLEALVSAESPSNDPALCAKAARVLFDIIGGAADETVSSGG
                     HEHYVWRWGGEPRVLLIGHLDTVWPAGTINRWPFTVDGERATGPGCFDMKAGLVIAGE
                     ALRALGSTEGVTLLVNTDEELGSGTSQSLIEAEAAGATATLVLEPGHDDAMKVARKGV
                     AMYVLRMRGRAAHAGLEPENGVNAIIEAAHQVRAIAALGDQSRGTTVTPTVLAAGSAR
                     NVVPALAVVEVDARFTDMAEAGRVDTAMRDLTASLPGAAIEVTGGVNRPPLERRMSER
                     LYALAREIGPSVGWVPGAAEVGGGSDGNYTAAMGIETIDGLGGRGGNAHAEGEWIDLS
                     SLPARARMLAALIERIRSA"
     gene            17293..17985
                     /locus_tag="VM841_12745"
     CDS             17293..17985
                     /locus_tag="VM841_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91093.1"
                     /translation="MTIKIRTLEGPAEMAALSDVFARIWGFTDTQVLPKEVFIGASIA
                     GQQVLGAFDGPRLIGGSWGFLTWDGSALGLHSHITGVLPPYRGGGGFALKMAQRDWCL
                     ARDITTVTWTFDPMIARNAAFNLRKLGATGVAFRPDFYGPMSDAFNAGEPTDRIEIVW
                     DLNAPPRPGPPAEAEAVEIPRDYLAFRETDRDAARQARAQATARMHELFEAGHRATWF
                     DGKTSRYLFTRR"
     gene            18198..18554
                     /locus_tag="VM841_12750"
     CDS             18198..18554
                     /locus_tag="VM841_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91094.1"
                     /translation="MRKLIALALTAVAALGATSTLANPGPNGSNNHGLCTAYFRGSET
                     GRENKRKAPPFAELERVSEEAYDAEFGEDNEATIDEKVAWWCGENAPHPGKGGGKSGG
                     NGGGNGGGGGKGKSGR"
     gene            complement(18736..19641)
                     /locus_tag="VM841_12755"
     CDS             complement(18736..19641)
                     /locus_tag="VM841_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HVL91095.1"
                     /translation="MTTNDIDGRVARLAARQFGVMSRAQVMATGMSPGMIKVRLRTER
                     WHEMYQGVYRVAGVPGSWRQRLMAATLACDGSGVSHEPAAALWALPSFPEQAIAVIVP
                     RGGRRSTQFVVHRATNLIAGDIVRLGPIPVTSAVRTFIDICATVSGARIEAALDDAIR
                     RGLLTAQRLVRRIDVLGRGRAGIRALRELALDRATLETVPQTVLESIMLSLVRAAGLP
                     DPCLQHSIGRWFADFAYPDARLVVEVDGFRWHGADRGRWQSDLARQNELIAAGWRVVR
                     FTWDDVTRRPAGVAATLRRMLQVNR"
     gene            complement(19771..20179)
                     /gene="rnpB"
                     /locus_tag="VM841_12760"
     ncRNA           complement(19771..20179)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VM841_12760"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(20200..>20858)
                     /locus_tag="VM841_12765"
     CDS             complement(20200..>20858)
                     /locus_tag="VM841_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL91096.1"
                     /translation="SIDAVGAGLATVLGNLQVVVVGVLAWLVLGEALDRRVVAAIPIV
                     VAGVVLVSGVVGAGAFGDDPAMGVIFGVGTSLAYAVFILVHREGARDLRRPAGPLFEA
                     SVVTAIVSVLWGLGDGSLDVVPSWPSHGWLLALALTAQVSGWLLLSISLPRLPAALSS
                     VLLLIQPVGSLVLGAIILGEDPSAVQFGGVALILAGVFLAARRRAPADAAAIPEPATP
                     "
BASE COUNT         3302 a         7338 c         7073 g         3145 t
ORIGIN      
        1 caacaaccgt ctgagcattg cgacctccta aggtgcgacg atctcgagca caccgctcgg
       61 gaccccgaag tggaccgaac agaagaacgg atagcggccg ggcgcgaggt tctcgacgcc
      121 ggcgaccggc accggggctt cgccgcggcg ggtccgcgcc gattcgaaaa agccgtcccc
      181 gtacgggtag tgctcgtcgg ggtcgccgga cacgatgtcg tgctcgtcgc cgtcggtgtt
      241 gaggagcgcg accgcgactc ccggcggaag acgcatctcg acccgcgccg tcctggtagg
      301 gggcgacagg tacacgtcgg tcgcgggcac gtacatgaag cgggccgcga cgatcaggaa
      361 tcggggcggc tcggccgctt gggcgggcag catcgccagc agcgacgaca gcgcgagaac
      421 gatgcagaca cgacgaatca ccgatggcct cccctcatcc ccctgtgatt tcggcacgaa
      481 cccagacaac cctttcgggc cggactattt tcgcagatcg ttgttccgtc ttgcccggct
      541 tggtccaggg atcgaccggc agggatcgtt ccaggaccgg gcgaactgct tgacagggac
      601 ttcggggagg aaaacggaat gaaacggcgc tcggggatgt tcgtcgcgct ggccgcggtc
      661 atcggctcgc tcctgcccgc cgccgcctcc cccccggtcg tgctgccgat gatccagccg
      721 ctccactggg acggctcggc gctgcgtgac cccgccggac gcgaggtgac gctgcgcggc
      781 ctcgaggtcg cggcagcgga gtccgcgccc gatgccgacc ggatcgcatc gtggggcttc
      841 aacctggtgc ggctcgggct gacctggcgt gaggtcgagc cggctcgcgg ccgctacgac
      901 cggacgtatc tcgcggcagt caagtcgatc gtgcacacgc tcgcgagccg cggcatctac
      961 acgctgatcc acttccggca agacctgtat cacgagcgct acggaggcga gggcgcaccc
     1021 gactgggcgg tcggctcgcc gctgccgccg gtcgataccg gaggcggccc ggccggctat
     1081 ctcacgcccg cggtcacgaa cgagtacgac ctgttctggg ggaacgcgag cggcgtgcgt
     1141 gacgcctacg ccgacgcgtg gatgcgtgtc gcggccgagc tgtcgggcga gccgggcgtg
     1201 ctcggctacg acctgttcgg cgcgccctgg cccggctcgc aatggccgtc gtgcacgagc
     1261 tccgctggct gccctgcctt cgacgatgtg atcctggacc gcttctaccg gcaggtgctc
     1321 ggcggcatcc gcacgatcga tgcggacgcg atgatctacg tctctcccgc gatccccgca
     1381 tcgctgggcg cgggcacatg ggtcgggccg gtcgacgacc cgtcggcgag caccggcctg
     1441 tcgtttcgcg tgacgtgccc ggcccggccg ccggaccacg gaccggccgg atccggcggc
     1501 gcgtgcggcc ccatcgagag ccgcgcgatg cgggtgcagt ccggccgggc gcgtgccgtc
     1561 ggcgcacatc cgatgctgag cggcgtcgcc gcgaacggcg acccggcctc cgctgaacgg
     1621 ctctttgatc tcgccgaagt gcacggcgtg tacagcttcg cctacgacgc gggtggcgag
     1681 gcctcgctcg cggccgatca cgccacgttg aaggtcgtcg cacgtcccta cgcgcagtcg
     1741 gttgcgggaa cgctgttcga gcaggactgg atgcccgacg agatgcttcg cacgctccgg
     1801 ctcgtttacc gcgcgaacgg cggcgacacc gtcgtggtgt ggccgcgcgc gatccaaggc
     1861 gagtacggcg ccggtgtcgt atgcaatccg ccgggccact gctcggtcac cgcggcaggc
     1921 gatcgcatcg tcgtgcacgc cgccgccggc gcgcacgtga tcttcagcgc ttcgcgcctg
     1981 gactgaccgc tacttgaagg ccgcgagccg gaagtactcg gcgttcatcg actcgctgaa
     2041 gacgtcctct ccgaacgcct cgagcatgcc ttcgacctgg ctcacgtgct cctcgatcgc
     2101 cttgagcttg agatcgagca agtcctgcgg cagcacgaag ttgatcgaca ggtcctcttc
     2161 cttatcgatg ggcgggccac cggcgaagaa gacgttgaag cgttcgaaca cgggcagcca
     2221 tcggtcggcc caggactgaa cggtcgtcgc gtagtggagc gtggcgcccg gcttcgccga
     2281 gcgggcgaac gccgccgtcg cccagcgcga gaccgcctga tgatcggtgt ggccggtcat
     2341 gccgtctggc ccgaacgtca ggatcgtgtc gggctcgacc tcctcgacga tcgccgcaac
     2401 gcgtgcgatg ccttccgcgt cgtcgatctc gtgcaggccg ccgtccggat agtcgagcca
     2461 cgaatgctcg tcgacgccga gcaccgccag gcaccggtcg agctccttct cgcgaatcgc
     2521 gccgagctcg gaggaaggcc agcggtcctc gtcccaggat ccgccttcgc cgcgggtcgc
     2581 ggttacgcac acgatgcgcc gcccttcgcg gcgcccggcc gccatgagcc cggcggacag
     2641 gtaggtctcg tcgtcgggat gcgcccacac tcccagaatg gttccgagct ccatccgcgt
     2701 cgctcccttc gcggctccgc ctagcggacc cgcaacacca gctcgatctg cttcgcctgc
     2761 ttcttcagcg tcggggttgc gttccgcccg agcgccccga tcgctttggc gatccaccag
     2821 ggcgcgccga tccgggagaa cacctccagc gcgtgccatg cctgcgcggc aaccccttgc
     2881 ggcggcgatc cttccgccgc gtgcaggcgc gcgcgcagca acgcctcgac gccggcgccc
     2941 agcccggtcg gccgcccgcc gcccatcttc tcgagcgccc gttcggccgc ggcgaactcg
     3001 ccggcctcga tccatcctcc cacgatcgtc tcgaccgccg ccagccagtg cggcatggcg
     3061 gccgcgccgt cgaacgcgcc gggcaggtcc tcgaagcgaa gcggcggtgc cgatgtcagg
     3121 ccgaaggccg cgaactgggc ggtcgcggcc gcgtccagca gccgtgcgta cggcgaggcg
     3181 gtgagcctgc gtcgctcgta ggcgtagaac gcgagcgaaa gccgcacctc gtcgcgccgt
     3241 gccgctgcga tcgggatcac cacgtgccag gcccgcagca tcgtcgtcgg atcctcggcg
     3301 cggttggcgt tggtgaccgc gcgcatcgcg gtggtgtgcg cgacgtccca gcggcccgcg
     3361 accattgcga tctcggcgcg aacggcctcg gcccaggcga tgtcccgcgg catggcctgc
     3421 gccgcggcca cgtctccgag gcgcgcggcg ggcgcggccg cgtccggcgc gtgatcgtcc
     3481 agcagcccca tcacctgcat gcgcaacgcg aacgccacgc gcagccagtc ctcgaggtcg
     3541 cgggcggcgc gctcgacggc gagccagtcc tcggcggtcg cgtcgccgat cgcggtcagc
     3601 gcctgcgcgc gaagcagcag cgcgtccagg acgagccggg aatccccgct cgcggtcgcg
     3661 tagaccagcg cttcgcccgc ttcggcgagc acgtcctcga gcgaggccgc ggcggtcagc
     3721 cccgtggagg ccttgtcaat aagcgtgcgt gcgcgcacgg cgtcggcgat cattcttgaa
     3781 agaacttacc ggagaagggg cctcgatact cggaacgctt gctcgacgaa gcgcgccggc
     3841 ccgagcacgg gtgagtagtt gcaggccggc caggccatcg atctgctcga tctcgtcgtc
     3901 catcgcggcg agcacggctg tcccctcggc atcgccgaag aggtgtgcca cggcgaaatc
     3961 cgtctcggcc tgcggacgaa cgccggttcc cacgggcgcc cagaaccggg caagcacccg
     4021 gcgaaccagc cgctgcgcct gcgggttgtc cttcagcagg acgcgggcct tggcgcgata
     4081 gaaggcatag tggcggcgct cgtccttgac gatgcgcgtg agcaactcac gcagcacagg
     4141 gttgcgcgtg gtcgcgatca aacgcttgta cccgtgcagc gtcgacagct cgttgatcgc
     4201 accccaggcc atatgcactg cgacgaagct cggcagcctc cgcgccgcga gcgtcttgag
     4261 ccaccgggcc accgctccgc cacgatcggt ttcacaccga atcgaggtga tccgcgccgg
     4321 agcatggcgc tctcccacgg cacgcaagaa cgcagccaga gcttcaccgt gccacagctc
     4381 ttcgtagacc cacagcgaga gaaacgcggt gatgtcggga tcgtacgccg cctggctcgc
     4441 gagcaggtcg cgcagataca cgaccgtgtg cgtttcgatg tccatcatgt aggttaggac
     4501 acgaatctct tcgcggctca cggggaagtc tgcggcgcgc cggaagtcaa taccttcgag
     4561 gtccaacttg cgcgacgcgc tcaggtactt ggccagatcg agatcgctca tcgtacgtcc
     4621 tccagagatc gcggggtcgc acgatgttcg tagccggctt gtgaacggtt cgggctaagc
     4681 gcgcagcttg accgaataga tcacgtccga tgtcggcgct tgcgtgccgg ccggaccggg
     4741 ctcgacggtg atcgcggccg cgtcgaatcg gcgtggatcg tggtcgagca gcaggatcgc
     4801 atcgcccccg ctcgcggtga acacgccggc ggcgaccggg gttccgttct gcatcagcca
     4861 cagttcgaag acacggtcgt cggggatgga cggcaatccc gatcccgtga gcacggcccg
     4921 tccgtcgttg gcgacggcca gcgtgaccgc gccctggccg tgcgggctcg tcagcgagaa
     4981 ccgctgcagc gtcggatccg acagcaccgc cagcacccgc tcgctctgct cgatgcgggc
     5041 ccgttcctcg cgcagatcac ccacgaccga atagctgaac aggcccagtg ccgcgaccgc
     5101 cgcgagcgag gcggccagca ttgcgcgcac cgcccagcga gagcgcgagg gctttgcggc
     5161 aacaggccgg gcctcaccgc ggatcgctcc catgaccttg gcttcgaacg cgggtgtgac
     5221 gggctcggcg ggcacaccca gggccaggtc gccggcggtg gcgcgaaagt cgtgatagac
     5281 gccgcggcag tcgtcgcaag ccgacaggtg gtcgagcagg tcgggcagga tgatcgcgcg
     5341 ttcggtctcg gacatcgagt ccagggcgta cgcggcaata gtctcttcga tctcgtgatg
     5401 cttggtcgtc acgactgctc acctcctcca tgcgatcggc gctctagggt atcccgcaat
     5461 cggcgcattg ccgcgacggt tcgtgacttg accgttccga gcggcacgcc gagcgcctcg
     5521 gcggcctgcg cctgcgtcaa tccttcgaag tacgtcttct ccagtacggc ccgctgctcg
     5581 tccggcaacg ccggcagcgc cgcctgcacc cgcgcgcgcc ggtcggcgag ccatgcgtcc
     5641 tcgatcacgt cgtcggcgct cggcagcggt ggctcgggcg cgtacgtggt cgtggtgcgc
     5701 cgccgctcgg cctcttcgcg ccggaccaca tcgaccgccc gatggtgcgc ttgcgcaagg
     5761 agccacgacc gcacggttcc gcgcgagccg tcatattgtc cggcccgctt ccatagcgac
     5821 acaaagacct cttgcacgac ctcctcggcc aagacacgat cgaccagcac tcgcgtcgcg
     5881 atgccgagcg ctcctggcgc gtagcggcga aacagatcgc cgaacgcgtg ctcgtctccg
     5941 cgtgcgatct tgccgatcga ggcgagatcg cgctgctgct gcagatcccc gacctcgtcc
     6001 gaggcggatc gatccgtgct catggcgtgc acgagtatcg cattccttcc tttacctgga
     6061 acattcacag gatgttcgga gcggatcgga cgccggttcg ggtcaggtca cgaccaccgt
     6121 tccgtgcatg ccggggacac cgggatgcct ggtacagaaa tactcgaagg ttccggcttc
     6181 gccgtaggtg aacgagaatg tcgatccggc atcgccgagc tgtccgtcat agtcgccggt
     6241 tacgtcgtcc ggtccggacc cggcggtcac ggtgtgcaga atctcatcct ggttgaccca
     6301 ggtcaccgag gtaccggcct tgaccgtgat cgagcccggg ctgaaggtga acatgcggat
     6361 ctcgacacgg tcttgcgtgg atgccgaggc cgcaggagcg tcgggatcac cggccgcgcc
     6421 gttgccggtc ccgccgcacg cggcgagcac cagcgccaag gccgcggcgg atgcgctcag
     6481 aagcagtggt cgtttcatga actggttcct ttcggaatcg gaacgcgcac gatcatgtcg
     6541 cgctcgaagt cggtgaccca aagggctccg gcggcctcga cgaggcggac ctgtccggga
     6601 tgcgatgcga gtgcgttgtc gaagaacagg atccgccccg tcgaaccgga cgccggatcg
     6661 acggacacga tggtcttgtc gtggttgttc gcgacccaga gccggcctgc cgccgcggtc
     6721 accgcgaccg ggcccagacc cggcaaggac cggtcgtcga cgctgatcgc gggtgcggcc
     6781 cggccggtgg cggtgtccac gcgcgtgagc gtcccggcgg tggcgccggt cacccagacg
     6841 ccgccgccgt cgatcgtgag cccggacgca cccgccggga ccgggaccgg cgtgccggcg
     6901 cgcctcgtcg cggcgtcgat gcgcacgatg gtccggtcga gcgcgttgct gacccacacg
     6961 gaatgctcgt cgccggcgag cgcgacagga ccgtagcccg tcgcgatcgg cgccgacgcg
     7021 gccatcgtgg ccgggtcgat tcgctgcacc tgcccctcgg tgacggtcgc aacccacaaa
     7081 ccggtcggca gcgcaacgac cgcgctcggg cccgttccga cgcgcaccga ggcggtgacc
     7141 acgttggtcg cgggatcgat gcgctgcacg acgtcatcgc cgtggctcgc gatccagacc
     7201 gcgccgaatc cataggcgat gccggcgggc tggcgggcaa cggggatcgt cgcgaccgtg
     7261 cgccgcgtcc gcggatcgac gcgggaaacg gttgcgtccc cgcggttggc gatccaaagc
     7321 gagccggcgc cctcggcgac gtcgaccggc tggcggccca cggcgatcgc gacgccgtcg
     7381 atcggcgagc tcgcactgcg cgtcgggtca cggccgggat cgaagtctcc gggtgcgcgc
     7441 agtccgtgaa ggtgcgcgcc ttcgacggac ggcagcccgt gccggacgtg ctcgtccaga
     7501 tgaccgtcgt ggtgcccgtg cgccgcgtga ccggcggccg catggtctgc gccggcgtga
     7561 cggtggccgg acgcaatcgg cgcggcggtt gcgatacccg cgctcagcag ggtcgccgcc
     7621 gctacggcgg cgatcgcgac actcggacgg ccacggcgga ccagcagccg ctgcggacgc
     7681 cgcagcagcg cgaacgcacc ggcggcggcg atcagctgga agccggccgt gagggagtcc
     7741 gcgatgccgg ccggttccac accgaggccg aagggaagtc cgatggttcg cgatacgacc
     7801 cacacccccg tgatagcaag gctgtgaagg gcgacggccg cgagcacgcc gcgtccggga
     7861 cgccgaacga ccgcgacggc caacgcggcc tgcgccagag cggtcgcggc gaagaacaga
     7921 ccgtggacag cggactcggc cgcgtgaccg ggcaataccg caaggtgcac gccggccgcg
     7981 gctgcgagcg caacgccggt caccgcggca aaccactggg tcatcgcttg tgccgtcctt
     8041 tcgaaagagt cggtgggaag accgggcgcg ccctcgcagg gacgcgcccg gcttccgcgg
     8101 ggagaagcgc gggggagacg cttctcgtta cttggcgaac ttgtcgggga actgagcggc
     8161 gatcgcgccc gccagcacct cggcgatgcc cggcatgacc tgcgcgcctt cttgcagctt
     8221 gacgaaagcc gttgggctct tggcggtcat cgcatcgatc gcgtcgaatg tggtgttgac
     8281 gtgcggcacc aacgcatcgg ctacggcatc cttcgtcagg ttcgggttcg cgctcgccaa
     8341 aaacgcgccg aagtcgttgc ggtacccgtc caggccttct cgcgccgtct tctgcttggc
     8401 ctcatcgttc gagagcttgc cgacggtgta gtcgacgaag aacccgatgt gcttgcgcca
     8461 cagcggcagg aacgacttgc cggcaccctc gtagaccgaa tcgatggcgt ccgacagcgc
     8521 gaccgagttg gcgtccagcg ccgcgctggc ggccttcgtc ggcgcggcat cgaggccgtt
     8581 cgcgaccgcg gtcgaaatcg tgatcccggc cagatactcg tgctcgacca gcaaggtcgt
     8641 cagagccgag cgaagacccg atccggccga tccagggtcg ccggcgatct tgtcggggaa
     8701 ctgcttggcg atcgcgccgg ccagcacctc ggcgatgccg ggcatgaccg ccgcaccttc
     8761 acgcagcttc gcgaacgcgt ccgggcttcc ggcgaccagc gcgtcgatcg catcgaaggt
     8821 cgagttcacg tgcggcacca acgcgtcggc cacggcttcc ttcgtcaggt tcgggttcgc
     8881 gctcgccagg aacgcgccga agtcgctgcg gtagccgtcc agaccctcgc gggcggtctt
     8941 ctgcttcgcc tcatcctttg agaccttgcc gaccgtgtag tcgacgaaga atccgatgtg
     9001 cttgcgccac aacggcagga acgatttgcc cgcgccgtcg tagacggaat cgatcgcctc
     9061 ggagagcgcc accgagttct tgtcgagcgc ttcgctcgct cccttggtgg cgtccgagtc
     9121 gaggccggtc gaaaccgcgg tcgagatcgt gatcccggcc aggtactcgt gctcgaccag
     9181 cagatcggtc agcgttgcgc gcagcccgga ggcggcgctg cccggccctg catcccgggt
     9241 cgcggtgccg ggcagcacgc tcgtggccgg ctttgcgacg gtatcggcgg ttgagccgca
     9301 ggcggcgccg gccagtgtca acgtcaacgc tcccgcggcg atcaccaaac ttttgcgctt
     9361 caaggtcaat ccactccctt cgtcctcgac gaggatgtcc cgtcaatcgg ctttcagcga
     9421 gtcttcggag acatcgcccg ttcggttcgt cggaagggga acttcttttg aaggcgaccc
     9481 ggttcggcgg acgaagacat cctgcccgca gcaagcggcc gcaagcggga ttcccttcaa
     9541 ttggggtgac gcgagtgccg tgaggtcgaa aggacgctag ccggcgtggg tgccgatcag
     9601 atcgagcact tccgagccgt aacgctggat cttggtcggg ccgattcccg agatgcggcc
     9661 gagctcgatc acgttggccg gccgctcttg cgcgatccgc gcgagcgtct gatcgtggaa
     9721 gaccacatag gcgggcacgc cgttcttgcg tgcggcttgc agccgccaat ctttcaaggc
     9781 cgtgaacagc gcgagatgct cgtcgccgac cgcgaccttg ggtcgcgact cggcctcgcg
     9841 cgacgctgcg gcagcccccg cgggcgttcc ggtcagacgg cccgaggtcg gaacggcctg
     9901 cggccgcatc tcggccagga acggcgagcg cttcggcgag cgccgccggt cccccggccg
     9961 ctgcgccgcc cacgacacga acaggtactt cttcgcgcgc gtgatcccga catacagcag
    10021 ccggcgctcc tcggcgatct cggcgtcgga cttcgacagc gcgaacggaa gctctttctc
    10081 ctccatgcgc ggcagaaaga cggcttcgaa ctcgagccct ttcgagcggt gataggtcag
    10141 gatctgcacg ccgcgtcctt cacgctcgac cgcgaagcgt ttgccgagat cttcgacgaa
    10201 cagctcggcg cgcgtcttga ccggcttcgc gtcgtcgggc tttgtggcgt cgtcgaactc
    10261 gtgcgcgagc gcccgcagcc ggcccaaatc cgcttgcctg gtcgcctcgc tcgcgtcggt
    10321 gtcgtcggga acctcttccc gccagccgag gccccgcgcg atctgatcca tcagcggccc
    10381 gacggccgtg tcgggagcgc gcttgagctt ggcgatcacc gatcgcgccg ccggccgccg
    10441 cagaaacgat ccgtcccgaa cctgataggg caggcggtcc gcactgaagg cctcctccag
    10501 atcttccgag cggccgttga tccggtacag cacggccatc tcttcccagg gcacgcccgc
    10561 gttgcgcagg gcccggcatt gccgcacgat ccactcgacc tcacccgatc cggtcgcgtg
    10621 ctcgcggaac accggttcgg gaccgtccgc gcccgctcgc gcggtcagcg acttcggcga
    10681 tccgcccatc ttcggcacca gacggttcgc gacgttcagg acctgcggcg tcgagcgata
    10741 gttgacggtg agccgaaaca cctgcgcgtc gtgccgttcg ggaaagtcga gcaggtaccg
    10801 cggcgtcgcg ccggtgaagc cgaagatcga ctggtagtcg tcgccgacca cgcacaggtc
    10861 gcgccgctcc cccacccaca cgtccagcag cgactgctgg agcaggttca cgtcctggta
    10921 ctcgtccacc gtgaacgcgc ggtacttggt gcggaccgcg ttcatcgcac gctcgtcggt
    10981 ccgcaaaagc tcgatcgtgc gggccagcag atcctcgaag tccatcaagc cggccgcttc
    11041 cttgcgacgc tcgtacgtct tatagaggcc gcccatcacc tcgaccggca ccggggtcac
    11101 ccgcgactcg gcggtcgcga cctcgatata acggtccggg ccgatcatcc ggttcttggc
    11161 ccactcgatc tcggtcgcga tgtcccgcac ggccgtgaac ttgtgcggtg gcggcagccg
    11221 cttgaccaat ggcgacagga tctggccctt cgacggcacg atctcggccg cgtccgacga
    11281 gaacgcctta tagagagcca gcgcctgcgc gtggaacgtc cgggcccgca ccgccggcac
    11341 gccgagtcgc tgcagccgcg cggccatctc gcgggcagcc ttgtcggtga aggtgaccgc
    11401 caggatctcg gacgcgtcga aggtcccggt cgcgatctga ttcgcgatcc gtcgcgtgat
    11461 cgtcgtggtt ttgcccgagc ccgccccggc caggatgcat accggcccgc gcacggccgc
    11521 cacggccgcc gcctgctgct cgttcagccc ctgaaagatt cggtccatcg ccgccgaggc
    11581 taacagcgcc cgccgacaac aacggtcagg gacgaaaact cgcaggcagc accctgtgga
    11641 taaccctatg tgtggataac cctatgggga atcggccaag ccctgagttg actgaataga
    11701 cggacgtccc cggtgtggtt cattcgccgc tcccccgccg tatctgggct agaaccaaaa
    11761 tagccacgac ggttgcgtac gctacgagga gtgacggcat ccactttcgc cgctcgcggc
    11821 gttatgcttg gccaccatga ccaaggcggc gttcttcgac ctcgacaaga cgctcgtgcc
    11881 cggctcgagc ttgttcttgc tggctcgcgg catgtatcag cgggactact accgtgtccg
    11941 cgacatggcc ggcttcgcct ggaaccaggt gatgttccgc ctggtcggct cggaacgcga
    12001 acaaggcatg gagcagtcga agaaggctgc gctcgagttc gtcaaaggca agcatcgcga
    12061 cgagttcgcg gcgctgggca acgagatcgt cgaagagcag atccttccgc gcgtctacga
    12121 agacatcgcg cgcgtcatcc gcagccatac cgacgccggc ttcgaaacgt tcctggtcac
    12181 cgccgcgccg atcgagctgg cgcgcgtcgt ttccgaaagc ctcggcatga ccggcgcgct
    12241 gggcaccgaa tccgagttcg acgacgccgg ccgctacacc ggccgtctgg tcggcgacat
    12301 cctgcacggc cccgagaaag ccaaagccgt gcaactgctc gcgttagagc gcggcatcga
    12361 cctgagcgag tcgcacgcgt atagcgactc caccaacgac ctgccgttgc tcgagacggt
    12421 cggccatccg catgccgtca atcccgacca cggcctgcgc cgcatcgccc gcacccgcgg
    12481 ctggcccatc cacgagctgc gtgctcgccg ccgtgccctg ctgatcggca ttcccagcgc
    12541 cgtcgccgcg accggcctgt tcgccggcgg tgtcgcactc ggcgcctggc tcgccaaccg
    12601 ccgccgcacg ctgtcatagc agccgagcct tcacgtgctt ttcttggccc accgcttctt
    12661 cagcaacgcc gtcgcgcgac gaacggcatc atggtcgcgg gcaaggggct cgacccgctg
    12721 aatgcggatc gattccagac tccagagtcc gacgacgccc catcgggcat cccgggcgct
    12781 ccaccacctg atcccggccc agcgcttttc ggcgaaaatc cgcgccgccc acgcctgggt
    12841 caccgtgcga tcgggcgtgg ccacaatgga gggacgaagc gcccgttcgg ccaacgcggc
    12901 ggcgtcgtcc agatcgacca cgcgcgcatc cacctgaacg gtgatcaggg acgacctggc
    12961 gtcgggcaga accggaaact cgaacatctc ctccatccag accggccggc tcgcgaacgc
    13021 ctcaccgacc gcgatcgcgg ggtcctcggc gaaatacagc acgcggtacc ggctcgggct
    13081 gtcgatgcgg gacgcgccct gcggcggcac gtgcagaggt tggccggggc cgccggcctc
    13141 cgccggctcg aggcccgcct ccggacgcca ctccagaaca ggagcgactc gatacaggat
    13201 cagaggaatc ctccctgcgc ctcgagcgcg agcgccgaat gcaccagccc gaactctccg
    13261 gcctcgatcg cctcggcggg ggtggttccg atgaactcgt tgtgcgcgtc gagccaatcc
    13321 agcgcgaccg ttgcgtgcat ctgtccgaac aagcgaaaca tcacatgatc gagatctccg
    13381 atccgcagaa tcatgtcggg ggacgggcgg tccttacttg atcgccaccg cgacgtttgc
    13441 gatgcgctga cgtcgagaag ccgggccacc cggttgtttc cgagcatcgc gatcagcgcc
    13501 tcgagccgcg agagcgctcc gcctacgccg ccgtagacgg gcgcgacctc gccgacccgg
    13561 acgggaccgg atcctttcgg tcgtcccggt cttcccatgc tcaattaata tcattgggca
    13621 ggaccccggg gccatcgatt tttccggccg agtcctcgtg cccactagca tggccggcat
    13681 ggccgccctc aacgatgttc gcgagtggct gcgcgaggcg cgcgcggtga cggttctgac
    13741 cggcgcgggg atctcgaccg agtcgggcat ccccgacttt cgcgggccga acgggttgtg
    13801 gacgcgcgac cccgaggccg agaagctctc cgacatcggc tggtacacct cgtcgcgaga
    13861 gatccgcgtc aaggcatgga aggcgcggct gcagcatccg gcgtggacgg cgaagccgaa
    13921 cgcgggccat cacgcgctgg tcgcgctcga gcgagccggc cgcatgcaca cgctggtgac
    13981 gcaaaacgtc gatgggctgc atcaaacggc cgggaccggc cccgagcgca tcgtcgagat
    14041 ccacggcacc gtccgcgagg tcgagtgcct gtcctgccaa gaccgcgctc cgatggaggt
    14101 cgcgctcgag cgcgtgcgcg gcggcgaaga cgatccggcg tgcaagcgct gcggcggaat
    14161 cttgaaatcc gcaacgatca gcttcggcca gtcgcttcgc cccgacgatc tggcgcgcgc
    14221 cgaacgcgcg gcgcgcgaat gcgacctgtt cctggccgtc ggaacctcgc tgacggtcta
    14281 tccggtcgcg gcgcttcccg aggtcgcgat ggcgcacggc gcacggctgg tgatcgtcaa
    14341 cgcggaaccg acgccctacg atccgatggc gcacgccgtg ctgcgagaac ccatcggcac
    14401 gatcctgccg gcgctggtcg gcacatgagc atccaggtgc ggccgctgtc ctcgctcgac
    14461 gagatcgcgg ccgccgacgc cgtcttcgat caagtctggc acgcacccga ccgcgagcgt
    14521 ctgccccgcg agctgctgcg cgccatgacc gcgcacacca atctgctgat cggtgcgttc
    14581 gacggcgaca cgtgcgtcgg cgtcgtgacc ggctggtggg gccgcgacga cgacgcggcc
    14641 gtctggctgt ggtcggcgcg gctgggcgtg ctggaatcgc atcgccgcgc aggcgtcgca
    14701 acgacgctca agcgcgagca ggcgcgcgtg gcgctcgagc gcggcgtgaa cgagattcgc
    14761 tggactttcg atccgatgcg cgcccgcaac gccgcgttca acatgcgcac gctcggcgcg
    14821 cgagcgatcg cctacctgga cgacgtctac gggccgcgtc aggaccggtt caatgcgggc
    14881 gagcggaccg accggctgct ggtgtcgtgg acgccgcacc ggcctgcgcc cgccggcaac
    14941 ccaccgttga cgcgggtccg cgtcccggcc ggcatcgacg agtatctttc gtggccgatg
    15001 gatcggcgcc gcgacgaacg cgaccgcgtc cgcaggcaga tgcggtcggc gttcgaggcg
    15061 ggactggtcg tcaccggatt cgaaccgccc ggcacctatc tgttcggagg gatcgcatga
    15121 tcacgcttcg cgccgccgag ctggtccgcg ccgacctgcc gctggtgcgc ccgttccgca
    15181 cctcgttcgg cgtgcaagag cttcgcgagg ccgtcctggt gcgcgtcgtg tgcgacgtcg
    15241 aaggcgtcga ggccgaaggg tggggcgaat gcgtcgccgg ctcggatccg ttctacagcg
    15301 ccgaatgggg cctgggcgcc tgggtgctgc tgcgcgaggt gctcctgccg atgctcgccg
    15361 aggagccctt gaccacgatc gaggacacac ctgccgcgat gagcgaggtg cgcggcaacc
    15421 gcatggccaa ggccgccgcc gagatggcga taaccgacgc ggcgttgcga gcgcgcggcg
    15481 tgtcgttgaa ggcgtatctg ggcggcgagc gggatcgcgt cgagtgcggc gtgagcatcg
    15541 gcatcatgga ctcgcccgac gatctggtcg ccgccgtcgg cacgtatctg ggcgatgggt
    15601 atacgcgcat caaatgcaag atcgagcccg aagccgacgt cgcgttcatc gcggccgttc
    15661 gcgaaacctt ccccgacatc gcgctgaccg tggacgcgaa cgcggcctac acgctcgcgg
    15721 acgcggatct gctggcccgg ctggacgcct tcgacctgga ctacatcgaa caaccgctcg
    15781 ccgaagaaga gctgatggag cacgccgcgc tggcgcgcct gatcgaaacg ccgatctgcc
    15841 tggatgagac gatcgtatcc gcgaagtcgg ccgaggacgc gatcgcgctg ggcgcgtgcg
    15901 aggtgatcaa catcaagcag ggccgcgtcg gtggcttggc cgaggcgtcg tggacgcacg
    15961 acgtgtgcgt cgagcacgag atccccgtct ggtgcggcgg catgctcgaa accggcctcg
    16021 gccgcgccgc gaacctggcc ttggcatcgc tgccgggctt caccatgccg ggcgacacca
    16081 gcccctcgtc gcgctacttc catgaagaca tcaccgcgcc gttcgagatg gcgcccgacg
    16141 gcacgatcgc cgttcccacc gggcccggcc tgggcgtcgc gccgctgccc gggatcatgg
    16201 ctcgcgtcgt cgccgaacga gagaaggtcg agctatgagg gacatgctcg aggcgttagt
    16261 ttcggccgag tcgccgtcga acgatccggc gctgtgcgcg aaggccgcgc gcgtgctgtt
    16321 cgacataatc gggggcgctg ccgatgaaac ggtctcatcc ggcggacacg agcattacgt
    16381 gtggcgctgg ggcggcgagc cgcgcgtgct gctgatcggc cacctggaca cggtctggcc
    16441 ggccggcacg atcaaccggt ggccgttcac ggtggacggc gagcgcgcca ccggtccggg
    16501 ctgcttcgac atgaaggccg ggctggtgat tgcgggcgag gcgctgcgcg cgctcggctc
    16561 gaccgagggc gtgacgctgc tggtcaacac cgacgaagag ctcggctcgg gaacgtcaca
    16621 gtcgctgatc gaggccgagg cggcgggcgc gaccgcgacc ctcgtgctgg agccgggaca
    16681 cgacgacgcg atgaaggttg cgcgtaaggg tgtggcgatg tacgtgctgc gcatgcgcgg
    16741 ccgcgccgcg catgccgggc tcgagcccga aaacggcgtc aacgcgatca tcgaggccgc
    16801 gcatcaggtg cgggcgatcg ccgcgctcgg cgaccagtcg cgcggcacga ccgtgacccc
    16861 gacggtcctg gccgccggct cggcgcgcaa cgtcgtgccc gctctggcgg tcgtcgaggt
    16921 cgatgcccgc ttcaccgaca tggccgaagc cggacgcgtc gataccgcga tgcgcgacct
    16981 gaccgcatcg ctgcccggcg cggcgatcga ggtaaccggc ggcgtgaacc gtccgccgct
    17041 ggagcgtcgc atgagcgagc ggctctacgc gctcgcgcgc gagatcggcc cgtccgtcgg
    17101 ctgggttccc ggcgccgccg aagtcggcgg cggcagcgac gggaactaca ccgccgcgat
    17161 gggaatcgag acgatagacg gcctcggcgg ccgcggcggc aacgcccacg ccgagggcga
    17221 atggatcgac ctgtcctcgc tgccagcccg cgcccgcatg ctcgccgccc tgatcgagcg
    17281 catccgttcg gcgtgacgat caagatccgg acgctcgagg gcccggccga gatggctgcg
    17341 ctctcggatg tgttcgcgcg gatctggggc ttcaccgata cgcaggtgct tcccaaagag
    17401 gtgttcatcg gcgcgtcgat cgcgggccag caggtcctgg gcgccttcga cgggcctcgt
    17461 ctgatcggcg gatcgtgggg gtttttgacc tgggacggca gcgcgctcgg cttgcactcg
    17521 cacatcaccg gcgttctccc gccgtaccgc ggcggcggcg ggttcgcgct caagatggcg
    17581 caacgcgact ggtgtctagc gcgtgacatc accacggtca cgtggacgtt cgatccgatg
    17641 atcgcgcgca acgcagcgtt caacctgaga aagctggggg cgaccggcgt cgcgttccgg
    17701 cccgacttct acggccccat gagcgacgcc ttcaacgccg gcgagccgac cgaccggatc
    17761 gagatcgtct gggatctgaa cgccccgccg cggccgggtc cccccgccga ggccgaggcc
    17821 gtcgagatcc cccgcgacta tctcgcgttt cgcgaaaccg accgagacgc tgcccgccaa
    17881 gcgcgcgcgc aggccacggc gcggatgcac gagctgttcg aggcgggcca tcgcgcgaca
    17941 tggttcgacg gcaagacgag ccgctacctg ttcacgcggc ggtagcggcg ctccccgtcg
    18001 agcaccgcga cggggacgag ggcctgaatc ggcgcatccc tcagagtcct ccctcgatcc
    18061 ggcagaaccg gcataactgg gtaaaggatc gaaacgcagc cgtggcgcgg ccatgaaaaa
    18121 ttcattaaag agacgcgaaa cgcatcgcga gggggcgtcg ttgagcaggt gcacacgacg
    18181 aactgaaggg agacgatgtg cgcaaattga tcgcgctcgc gctgacggcc gtagcggctc
    18241 tgggagcgac gagcaccctg gcgaatccgg gaccaaacgg ctcgaacaac cacgggttat
    18301 gcaccgcgta cttccggggt tccgagaccg gacgcgaaaa caagcggaag gcaccgccgt
    18361 tcgccgagct ggagcgcgtg tccgaggagg cctacgacgc cgagttcggc gaggacaacg
    18421 aggccaccat cgacgagaag gtcgcctggt ggtgcggcga aaacgcgccg caccccggca
    18481 agggcggcgg caagagcggc gggaacggtg gcggcaacgg tggcggcggg ggcaagggca
    18541 agagcggccg ctaagcccgc atctcgtcgc gaagcggagg ggcagccccc gggcccctct
    18601 gcttcgtcac ctccggcacc gacgtctcgc cccctggccc gagatcgaag tcgccgaccg
    18661 actgcgcggc gccatcatgg agcggtgggc gccgaccggc cgcatcgagg tcgtctccag
    18721 accctcaacg ccgagctacc tgttcacctg gagcatccgg cggagcgtgg cggccacacc
    18781 tgcgggacgc cgggtgacgt cgtcccatgt gaagcgcacc acacgccatc cggctgcgat
    18841 cagttcgttc tgtcgggcga gatcgctctg ccatcggccc cggtccgcac cgtgccagcg
    18901 gaagccgtcc acctccacga cgagccgggc atccggatag gcgaagtccg caaaccagcg
    18961 gccgatcgag tgttgcagac acggatccgg cagccctgcg gctcgaacga gcgagagcat
    19021 gatggactcc aggacggtct gcggcacggt ttccagcgtc gcacgatcaa gggcgagctc
    19081 gcgcagcgcg cggatccctg cgcggccgcg accgagcacg tcgatccgcc ggacgagccg
    19141 ctgcgcagtc agcagaccac gccggatcgc atcgtcgagc gcggcctcga tccgcgctcc
    19201 ggaaacggtc gcgcatatat cgatgaacgt acgcaccgcg gaggtcaccg ggatcggccc
    19261 aagacgcacg atgtctcccg cgatcaggtt cgtcgcccga tgcacgacga actgcgtcga
    19321 acgacgtccg ccgcgtggga cgatcaccgc gatcgcctgc tcgggaaacg acggcaaagc
    19381 ccacagtgct gccgcaggct cgtgggagac gccggatcca tcgcaggcca gcgttgcggc
    19441 catcaaccgt tgccgccacg aaccgggcac gcccgcaacg cgatacacgc cctgatacat
    19501 ctcgtgccac cgctcggttc gcagccggac cttgatcatc ccgggagaca ttcccgtggc
    19561 catcacctga gcgcgagaca tcacgccgaa ttggcgcgcc gcgaggcgcg cgacgcgccc
    19621 atcaatgtcg ttcgtggtca acttggggtc ctataggtgc ccgaggttga ccaagaaagc
    19681 acagccccaa ccccatgtga agcccacgcc ggcaccttcg aggagttcgg gggaaatgag
    19741 ggaggccccc gctcgcgcgg gggccccgag atcggaagcg ggcctgtaag cgggcttctg
    19801 tcccgagccg ccgccgaagc ggcggccggg ggcggccatc tctctgggac caccgttgcc
    19861 ggcggcctcg tgcggcctac ccggaagcgt tgcggacggg ccgcccgccc cgcgacccga
    19921 aagtcgcagg cgcttcctgc ttggccttgc tccggacggg gcttgccgag ccgcgacggt
    19981 cacccgccgc gctggtgagc tcttacctca ccgttgcacc cttaccggcc cggtcgaaac
    20041 cggcccggcg gtatcttttc tgtagcgctt tccccggctc gcaccgggtg ggcgttaccc
    20101 accgtcctgc cctgcggagg cccgactttc ctcgacgccg cacccgaagg caccacgccg
    20161 cggccgcccg gcctgcttcc gacgaccagt gtacgcccgc tagggcgttg cgggctcggg
    20221 aatcgcggcg gcgtcggccg gggcgcggcg gcgggcggcc agaaacaccc cggccaggat
    20281 cagcgcaacg ccaccgaact gaacggcgct ggggtcctcg cccaggatga tcgctcccag
    20341 caccagcgag ccgaccggct ggatcagcag cagcacgctg ctgagcgcgg ccggcaggcg
    20401 cggcagcgag atcgacagca gcagccatcc cgaaacctgc gcggtcaacg cgagcgcgag
    20461 cagccagccg tgcgacggcc acgacggcac cacgtccagg ctgccgtccc cgagccccca
    20521 cagcaccgac acgatcgcgg tcacgaccga cgcttcgaac agcggcccgg ccggccggcg
    20581 cagatcgcgc gcgccttcgc ggtgcacgag gatgaacacc gcgtacgcca gcgacgtgcc
    20641 gacgccgaag atcacaccca tcgcgggatc atccccgaac gctccggcac ccaccacgcc
    20701 cgacaccagc accacgcccg ccaccacgat cgggatcgcc gccacgacgc ggcggtcgag
    20761 cgcctcgccc agcacgagcc acgcgagcac gcccacgacc accacctgca agttgccgag
    20821 caccgtcgca agccctgccc ccaccgcgtc gatcgagt
//
LOCUS       DATLFO010000315        18077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_66943, whole genome shotgun sequence.
ACCESSION   DATLFO010000315 DATLFO010000000
VERSION     DATLFO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66943"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VM841_12770"
     CDS             complement(<1..760)
                     /locus_tag="VM841_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVL91097.1"
                     /translation="MSRLLVLPVAIPLLAAALTLLMRSKLAIQRALSVAASAAVLAFA
                     IALVARTAGGEALAARIGGFPGPFAISFAADTFSALMLTVAALMVLVCTAFAIASRDR
                     ERPLLHPLILVLEAGVSGSFLTADLFNLFVFFEVMLISSYVLLTLGGSKSQVRSGTIY
                     VATNLLGSTILLAGVGLTYAIAGTVNMASLASLEDPLRRLAVPGAMLLIAFAVKAALV
                     PVHGWLPSAYPSARPAVAALFSGLLTKVGVYALYR"
     gene            complement(757..1071)
                     /locus_tag="VM841_12775"
     CDS             complement(757..1071)
                     /locus_tag="VM841_12775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HVL91098.1"
                     /translation="MSVAAVIGILAAAGCYLLMQRGIVRIALGFVLLGHAANVLLVSS
                     GGVARRGVPIVGAAGTPADPLPQAFALTAIVIGFAVTAFLLALAFRYDEQTGNDDPED
                     ES"
     gene            complement(1068..1496)
                     /locus_tag="VM841_12780"
     CDS             complement(1068..1496)
                     /locus_tag="VM841_12780"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HVL91099.1"
                     /translation="MSTVILRTTARLVTPGIVGASLYLLLRGHNAPGGGFIGALVAGA
                     AIVLRYLVGGPVAVRRMLPVSAESLLGVGLLIALMTGIGSLIAGSEFLTSTIWSARIP
                     GIGKVKVASSLAFDIGVYLIVLGVIVGVVGFLGEDDQVMQ"
     gene            complement(1493..3787)
                     /gene="mbhE"
                     /locus_tag="VM841_12785"
     CDS             complement(1493..3787)
                     /gene="mbhE"
                     /locus_tag="VM841_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HVL91100.1"
                     /translation="MSATASGWVLAASFATAAVAPLLARWFGRNTGYALGMLYAAGGA
                     ILAAQAPAIVRGEPIVASMEWIPSLDVRLSISLDGLSLLFSLLILWIGAIVSVYSARY
                     FSPGGSVGRHYGLMSLFAASMLGLVLSDNVIAMFVFWELTSITSFFLIGGNGKTGSKQ
                     AMRAFLVTGLGGLALLGGLLLMSVVAGSTSLREIFAGGSAIVDHAAAPAIMALIILGA
                     FTKSAQMPFHFWLPGAMVAPTPVSTYLHSATMVKAGIYLLARMSPLFGGQRGWMIPIV
                     LTGLITAVAGAAIALKQHDLKALLAYSTVSQLGFITALVGVGSFYALAAAAAHTMAHA
                     LYKATLFMIVGVVDHQAGGRDIRELHGLGRMMPITATVAALAAMSMAGLPPFFGFVSK
                     EESFGALLAAPGAGWIAPVAAALAVLASMLTFAYSARLIEGAFGGQPTRPLAEPPRGL
                     LAPAAVTAVAGLVLTGALPRLISLLDRIAQDAVGTAGHVKFALWHGITPALGLSVITI
                     TGGLALYALRRRIDTGFDRVRLPASGTGSFDAVYDTIVAAGRAAGRPFISRAAAIHAS
                     WVLGAVIVAAGGAWLAYRTAQPPPVAATPGEDWLIVALIAVAAGAAIRARSRIAAVAL
                     IGATGFLIAVLYVLLGAPDLALTQLLVETLTVTLVVMVFRRLPRRFEPVRGARRLRAA
                     GIAIVTGTLAGAAAYAFAGRRPLSDVAGYYLEAAEPVTGGSNVVNTILVDFRALDTLG
                     EITVLAVAALGVYALVRLAKGGGA"
     gene            4508..5425
                     /gene="holA"
                     /locus_tag="VM841_12790"
     CDS             4508..5425
                     /gene="holA"
                     /locus_tag="VM841_12790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HVL91101.1"
                     /translation="MRPVTVILASDEFVASESLDRLRAEAAAEGLVAEEIDPGDPQAV
                     RYALDTPSLFGGGRFVLVRDAHTLDEDAQDAIEAWAKNPADGVVLALMASGTTAAKLS
                     KRFPAGAIIKAESVPPWKAAEWAVKRAKEKGRRMSGPAAAALVESVGSDLRDLAAAID
                     RLVETSKAATIELDQVQERFTGVESKVYQFVDSVFDRDLPLARKRLHGLLDQGENPIG
                     LLAALARQLRVIAYVSGNDGRQAAAIAKDLGLKGEGAVKRAIRQARGFNPARIAKAYR
                     VMADADLAMKSEGDDPLVLELVVDEIAAR"
     gene            complement(5435..5713)
                     /gene="rpsT"
                     /locus_tag="VM841_12795"
     CDS             complement(5435..5713)
                     /gene="rpsT"
                     /locus_tag="VM841_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVL91102.1"
                     /translation="MANIKSQIKRNRQNERARVRNLSTRSRLKTLTRRFREALAAGDL
                     DAAGAALKTVCRAYDKAASAGVLHANNAANHKSKLQKAFNTAKSATAA"
     gene            5858..6802
                     /locus_tag="VM841_12800"
     CDS             5858..6802
                     /locus_tag="VM841_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL91103.1"
                     /translation="MTEPPRPVLFEDVPYAARDGEILLARIYRVADAGAATAVVDVHP
                     GAWSEGDRTWGRVYGEALARRGFVVVALDFRQGPRFAHPAASADVATGVRWVRAASEP
                     LGIDPGRIALTGSSSGGHLAMLAACRPNAPEHAGAPLRWDRSGEVPGEIDASVRCVAA
                     LWPPVDPGARYEWLLERLRTEPPDQHDHYRSLLDGTNSYFGDRGVMRAVSVPRIVRDG
                     EATHLPPVWVCYPELDQNVPSFIVEDLEAAWRAAGGTISVSVYEGAEHAFGHWPGAAT
                     DRFVDELAAFLRRFTEPGEANTSSRTSDVRSSFRSEGR"
     gene            6815..8635
                     /gene="lepA"
                     /locus_tag="VM841_12805"
     CDS             6815..8635
                     /gene="lepA"
                     /locus_tag="VM841_12805"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HVL91104.1"
                     /translation="MPTDQHLQRDFCIVAHVDHGKSTLADRLLEVTHAVDDRQMRAQY
                     LDKMDLERERGITIKAAAARINYAAKDGKTYVLNLIDTPGHVDFSYEVSRALNACDGA
                     VLLVDASQGIEAQTLANLHLAIEADLEIIPVINKIDLPAAQPEKIANELKQVIGCSDD
                     EIIFVSGKTGEGVTELLEAIVEKVPPPSGDPLGPTRALIFDSMYDSYRGVITYIRVVD
                     GEIHKGGRVRLMATEAEHDVEEIGFLTPEPVPVDTLGAGEVGYLITGIKNVREAKVGD
                     TVTDARSRAPQPLPGYRDAKPMVFAGLYPAQGDDYPQLRDALDRLQLNDAALVFEPET
                     SKALGFGFRCGFLGLLHMEITQERLEREFDLELVVTAPNVAYRLFMTDGTEAGVRNPS
                     DMPDPSKIERIEEPMVRAMVLSPTEYVGTIMDLCMTRRGVLDGMHYLSTERVELKYRL
                     PLSEIIFDFFDQLKARTRGFASLDYEPIGYQAADLVRVDILLHGDTVDAFSAIVHREK
                     AQVYGRNMVERLRKLIPRQMFDVPVQAAIGSRIISRETIKAKRKDVLSKCYGGDITRK
                     RKLLEKQKKGKRRMKQIGSVEVPQEAFIAALQVGEIEEPD"
     gene            8635..9753
                     /gene="hemW"
                     /locus_tag="VM841_12810"
     CDS             8635..9753
                     /gene="hemW"
                     /locus_tag="VM841_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HVL91105.1"
                     /translation="MAHAALLAPRFGIYVHVPYCLSVCPYCDFNVYVGSAADADAYLD
                     AVLIEADTRRFELRGEAGTVFLGGGTPSLISPPALRRFLDSLRSILPVSPGAEITLEA
                     NPETVTVSSLEGYRTAGVNRLSLGVQSFAPHVLSALGRTHTPPDAGNAVAFARSAGFD
                     NVSVDLIFGTAGESLADWSSTLSSVVASGVDHVSCYGLTIEDGTAFGAAVTRGALSPP
                     DEDDLASKYEMACEMLPLRHYEISNWGEPSRHNLIYWTQGDYLGLGAGAHSHREGLRS
                     WNRRLPRAYMTDPAHPVAGSEELGDAGKAREWLLLRLRLVDGVDLAQAAIVLDVDVRS
                     LATPIVEAGLAVMDGDVMKLTPRGLLLENEIALRLGAA"
     gene            10102..11268
                     /locus_tag="VM841_12815"
     CDS             10102..11268
                     /locus_tag="VM841_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91106.1"
                     /translation="MRSSLDRITAEVRTLVDRVATVPSSDVAGVLDAFERLSRVVAAG
                     RLAALAAVERSRTWRGEGHASFAHWAAERTGTTVGHALGAVTAAKTLASLPATREALA
                     SGTLSVQQASEIAGAAAVDPASEPGLLGAAAESSASELRERCRMVKVAAAGNDAYERI
                     RRTRYLRFQSDPDDAVRVEGRLVPDAAAALMMWCRAEADRRAAIGRRDGTIEPHEALM
                     ADALCSLPGTVSSGSAPRAKDAIVRSTVYVHVDAAAWERGSASPGERCEIAGVGPTTV
                     AAARRLAAQPGGTLKIAIRDKGNVVGVVNTGRYVPARVGAALEVRDERCVVKGCTRRR
                     GLQRDHIVEYGRGGETSLANLQRVCGYHHDLKTHMGWKIVGEPPNCRIEPPAPP"
     gene            11383..12405
                     /gene="hrcA"
                     /locus_tag="VM841_12820"
     CDS             11383..12405
                     /gene="hrcA"
                     /locus_tag="VM841_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVL91107.1"
                     /translation="MGNPNEPLADRQGDILVAIVREYIRTADPVGSKHLVGKYNLSVS
                     AATVRNEMARLEELGYLHQPHTSAGRVPTDAGYRFFVDHQRPARLGSQHRAEMQSAVG
                     AEPADMDELMRRASEALSRATNLAAAALTPRLRTSRLKHLDLAWLGPRRLVVIVIADG
                     GRVEKRIVELESDVSEATLEAAGDELNRLLCGSTVDGAERAARERCEAEAAGAGDAAV
                     AGAVASALKAFLDEDQELVVGGAATLAGAFGGDQDAFPRVFEVIERRIDLQPLMSDAQ
                     PPASVRIGSEVPVEDLQMCSLIFAPYSHGAAVGVIGPTRMEYPQVIAAVSMMARLLNN
                     AVQDLS"
     gene            12417..13535
                     /gene="dnaJ"
                     /locus_tag="VM841_12825"
     CDS             12417..13535
                     /gene="dnaJ"
                     /locus_tag="VM841_12825"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVL91108.1"
                     /translation="MADPYAMLGVSRSASPEEIKKAYRRLARQYHPDTSAEPDAETRF
                     KQINAAYEVLSDPERRQRYDMFGDDGSRAASAGSGAGANAGFGDLGDLFESFFGGGGF
                     GGGGRRRGPRSVAERGADIETTVRLTIDEAVFGVKHELEVFAALACEVCHGAGVQEGT
                     GHVRCGTCEGTGEIRQMQRSIFGAMMTARACGRCRGTGQVPEAPCPSCAGEGRMMRSM
                     MVTVDIPAGVEHGTTLRVRGRGEAGVRGGPPGDLYVHIGVEPNETYHRKGDDLLCALR
                     VPLTQALLGATVSVTTLEGEEEPIELEPGTQPGAVYRIKGRGVPKLGGRGRGDLVIEI
                     DVEIPRKLSGEQRDLVRRLAELRGELVAEKPRKRGFLR"
     gene            13674..14390
                     /locus_tag="VM841_12830"
     CDS             13674..14390
                     /locus_tag="VM841_12830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HVL91109.1"
                     /translation="MSAHHFFLPSIEGVLLTIEGEDARHATRVLRIRPGEAITVSDGR
                     GGIATATVTDASRALSASVMDRRVVEPIRPALTVYQALPKAGKLERVVQALTQCGVAA
                     IVPLRAARSIAKWDAAKAASNVGRLRAVAREAAMQSRRAWLPTVRDPIGIDEVPAGTI
                     VLHEAASTRLSALLPLASGAPAAVSIAIGPEGGFDDAEIAALEARGCPAATLGPVVLR
                     TETASIVAAAVILARYGSLG"
     gene            14581..15072
                     /locus_tag="VM841_12835"
     CDS             14581..15072
                     /locus_tag="VM841_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVL91110.1"
                     /translation="MNSTYAKRVGERLRAIRLQKGYSLHDVEDVSSKEFKASVLGAYE
                     RGERSISVPRLQRLAQFYGVPVDQLLPKLEGETIVLDGRGPQEGGIRLDLTKLRETNG
                     AETDAVSRYVTMIQMARGDFNGRVLTVRTEDLRALAAALDLTPQALKSRLDELGVRIP
                     ALV"
     gene            15100..16074
                     /locus_tag="VM841_12840"
     CDS             15100..16074
                     /locus_tag="VM841_12840"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL91111.1"
                     /translation="MRALVTGATGFIGGRLVPRLVERGHRVTALVRSPDRAAGLREMG
                     VTLAGGDITEPATLEGPMRKADAVFHLAAWYEMGVGDRTKMYQVNVRGTEHVLAAARE
                     AGVGRIVHCSTVGALGAGPIGDIRSETSQHSGAFGSVYEETKWQAHQVARRAAADGAP
                     VVIVMPGAVYGPGDPSVLGAMIRLYAKGWLIALPFQDSGFSWVHVDDVAEGIVLAHEK
                     GRAGEEYVIGGANATVGDVFRQLAPLTGIRAPRFELPGWMLRASMPLSPVIARTLNAG
                     PRIVQDALSSMRGSFMAFSTKAEAELGYRHRSIEEGMPETVEWFKENR"
     gene            16134..16475
                     /locus_tag="VM841_12845"
     CDS             16134..16475
                     /locus_tag="VM841_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HVL91112.1"
                     /translation="MEDCLFCKFVSGEIAPDVVYQDDLVLAFRDINPQAPTHVLIVPK
                     QHTKDASTVEPSDGPALAAITNAARTIAAQEGLPGGYRLVANVGPDAGQSVFHLHVHM
                     LGGRSLAWPPG"
     gene            16672..17592
                     /locus_tag="VM841_12850"
     CDS             16672..17592
                     /locus_tag="VM841_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVL91113.1"
                     /translation="MVGLVGERDELLKVVEAAFEAQILVRGNEITISGEPGHVERVST
                     LFEELIELLERGTVLTADSLTRSIDMVKGIAGETARPSQVLSDAVLTHRGKAIKPKTL
                     GQKKYVDAIRKSTIVFGIGPAGTGKTYLAMAMAVKALQEKQVARLILTRPAVEAGEKL
                     GFLPGTLYEKIDPYMRPLYDALYDMMDPESLSRLTQRGTIEVSPLAFMRGRTLNDSFI
                     ILDEAQNTTPEQMKMFLTRLGFGSKVVVTGDVSQIDLPSGQSSGLNVVREILTEIDGL
                     EFVFLGSKDVVRHSIVQEIVEAYRRFDEAH"
     gene            17643..>18077
                     /gene="ybeY"
                     /locus_tag="VM841_12855"
     CDS             17643..>18077
                     /gene="ybeY"
                     /locus_tag="VM841_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVL91114.1"
                     /translation="MSDPDDPGGPGSLPGGPVTVALADEQTIPVDPDALVHAARRTMD
                     ILGMRGELSLALVDPARIAGLKRDYYGEAAATDVLSFPMDGAGGPMIGDVIICPAVAL
                     RQARGMHQRIGIDGNGLLVGEGHRNGAAGKTSRSAGIVGVAHG"
BASE COUNT         2933 a         6021 c         6502 g         2621 t
ORIGIN      
        1 cccgatagag cgcgtagacg ccgaccttgg tgagcaagcc cgagaacagc gcggcgacgg
       61 ccggccgcgc ggacgggtac gcgctgggca gccatccgtg aacgggcacg agcgcggcct
      121 tgacggcgaa ggcgatcagg agcatcgcgc ccggaacggc gagccggcgc agcgggtcct
      181 cgagcgatgc gagcgaagcc atgttgacgg tcccggcgat cgcataggtc aggccgacgc
      241 cggccagcag aatcgtcgag ccgagcaggt tcgtggcgac gtagatggtg ccggaccgca
      301 cctggctctt cgagccaccg agcgtgagca gcacgtacga ggagatcagc atcacttcga
      361 agaacacgaa caagttgaac aggtccgcgg tcaggaacga gcccgacacg cccgcttcca
      421 ggacgaggat caacggatgc agcagcggcc gctcgcggtc ccgggacgcg atcgcgaacg
      481 cggtgcagac cagcaccatc agcgccgcga cggtgagcat caaggccgag aatgtatcgg
      541 ccgcgaacga gatcgcgaac gggcccggga agccgccgat gcgcgcggcc agcgcctcgc
      601 cgccggcagt gcgcgcgacc agcgcgatcg cgaatgcgag caccgcggcg ctggccgcga
      661 cgctcagcgc gcgctggatc gcgagcttgc tgcgcatcag cagcgtcagc gctgcggcga
      721 gcagcgggat cgcgaccggc agcaccagca gccggctcac gattcgtcct cggggtcgtc
      781 gttgccggtc tgctcgtcgt agcggaacgc cagcgcgagc aggaatgcgg tgaccgcgaa
      841 gccgatcacg atcgccgtca gcgcgaaggc ctgcggcagc gggtcggcgg gggttcccgc
      901 ggcgccaacg atcggcacgc cgcgccgcgc cacgccgccc gaggacacca gcagcacgtt
      961 cgccgcgtga cccaggagca cgaaacccag cgcgatccgc acgatgccgc gctgcatgag
     1021 caggtagcag ccggccgcgg ccaggatccc gatcacggcg gcgacgctca ctgcatcacc
     1081 tggtcgtctt cgccgaggaa tccgaccacg ccgacgatga cgcccagcac gatcagatag
     1141 acgccgatgt cgaacgcgag cgaggatgcg actttgacct tgccgattcc cggaatccgg
     1201 gccgaccaga tcgtactggt caggaactcg ctgccggcga tcagcgatcc gatcccggtc
     1261 atcagcgcga tcagcaaccc gacgcccagc agcgactcgg ccgacaccgg cagcatgcgg
     1321 cgcacggcga ccggtccgcc gacgagatag cgaagcacga tcgccgcgcc ggcgaccagc
     1381 gcgccgatga acccgccgcc cggcgcgttg tgtccgcgca gcaacaggta caacgacgcc
     1441 ccgacgatgc ccggcgtcac gagccgcgct gtggtgcgca ggatcaccgt gctcatgcgc
     1501 cgcctccttt cgcgagccgc accagcgcgt acacgcccag cgcggcgacg gccagaacgg
     1561 tgatctcgcc cagcgtgtcg agcgcgcgga agtccaccag gatcgtgttc accacgttgc
     1621 ttccgccggt gaccggctcg gcggcctcga ggtagtagcc ggccacatcc gacagcggac
     1681 ggcgtccggc gaacgcatag gcggccgcgc cggccagcgt gcccgtgacg atcgcgatac
     1741 cggcggctcg cagccggcgt gctccgcgga ccggctcgaa gcggcgcggc aggcggcgga
     1801 acaccatcac gaccagcgtg acggttagcg tctcgacgag cagctgcgtc agcgcgaggt
     1861 cgggcgctcc gagaagaacg tagagcacgg cgatcaggaa gccggtcgcg ccgatcaacg
     1921 cgaccgcggc gatccgcgag cgcgcccgga tggccgcgcc ggccgccacc gcgatcagcg
     1981 cgacgatcag ccagtcctcg ccgggggtcg cggcgaccgg cgggggctgt gcggtgcgat
     2041 aggcgagcca tgcgccgccg gccgcgacga tcaccgcacc gagcacccag ctcgcgtgga
     2101 tggcggcggc tcgcgagatg aacggccgcc cggcggcgcg cccggccgcc acgatcgtgt
     2161 cgtagacggc gtcgaacgag cccgttccac tcgcaggcag ccgaacgcga tcgaacccgg
     2221 tgtcgatgcg ccgtctcagt gcatagagcg cgagcccgcc cgtgatcgtg atcaccgaca
     2281 accccagcgc gggcgtgatt ccgtgccaga gcgcgaactt cacgtgcccg gccgttccga
     2341 ccgcgtcctg cgcgatgcga tccagcagcg agatgagccg cggcagcgcg cccgtcagga
     2401 ccaggcccgc gacggcggtc accgcggcgg gggcgaggag gccacgcggc ggctccgcga
     2461 gcgggcgcgt cggctgaccg ccgaacgcgc cttcgatgag gcgcgcgctg taggcgaagg
     2521 tcagcatcga ggcgagcacc gcaagggccg ccgcgaccgg tgcgatccag ccggcgccgg
     2581 gcgcggccag gagcgcgccg aacgattctt ccttgctgac gaagccgaag aacggaggca
     2641 gcccggccat cgacatcgcg gcaagcgccg ccacggtcgc ggtgatcggc atcatcctgc
     2701 ccaagccgtg cagctcgcgg atgtcgcgac cgccggcctg gtggtcgacc acgccgacga
     2761 tcatgaagag cgtcgccttg tagagcgcgt gcgccatcgt atgcgcggcc gctgcggcga
     2821 gcgcgtagaa cgagccgacg ccgaccagcg cggtgatgaa gccgagctgg ctcacggtcg
     2881 agtacgccag caacgccttc agatcatgct gtttgagcgc gatcgccgcg ccggcgaccg
     2941 cggtgatcaa cccggtgagc acgatcggga tcatccagcc gcgctgcccg ccgaacaggg
     3001 gcgacatgcg cgcgagcaga tagatcccgg ccttgaccat cgtcgccgag tgcagatagg
     3061 tgctcaccgg ggtgggcgcg accatcgcgc cgggaagcca gaagtggaac ggcatctgcg
     3121 ccgacttggt gaaagcgccc aggatgatca gcgccatgat cgccggcgcg gcagcatgat
     3181 cgacgatggc gctgccgccg gcgaagattt cgcgcaggct cgtgctgccg gcgaccaccg
     3241 acatcagcaa caagccgccg agcagcgcaa gcccgcccag ccccgtcacc aggaacgcgc
     3301 gcatcgcctg cttcgagccg gtctttccgt tcccgccgat caggaagaac gacgtgatgc
     3361 tggtcagctc ccagaacacg aacatcgcga tcacgttgtc cgagagcacc agcccgagca
     3421 tgctcgccgc gaacagcgac atcagcccgt agtgccggcc gacggacccg ccgggcgaga
     3481 agtaacgcgc cgagtacacc gacacgatcg cgccgatcca cagaatcagc agcgaaaaca
     3541 gcagcgagag cccgtcgagc gagatcgaca gccgcacgtc cagcgacggg atccactcca
     3601 ttgaggcgac gatcggctcg ccgcgcacga tcgcgggagc ctgcgcggca aggatcgcgc
     3661 cgcccgccgc gtagagcatc cccagcgcgt agcccgtgtt gcggccgaac catcgcgcca
     3721 gcagcggcgc aaccgcggcg gtggcgaagg acgccgcgag cacccatccc gaggcagtgg
     3781 cgctcacgcg tgcgatctcc cggccgcggg gcaggacgga gccgcgatcc gcttcaacgg
     3841 gcgcgcgaac attcggtccg gacggtcatc gttcccgcga tgatagtcgg caatcgcgca
     3901 gcgctccctc cttgcgcggt ggacccaggg gggctccgac cctgtgcaac caagttgcac
     3961 gacggaccga ggtgggcccc gaccctgtgc aaccaagttg cacgacggac cgaggtaggc
     4021 cccgaccctg tgcaaccaag ttgcacggga gctgcgggag ctgcgggagc tgcgggagct
     4081 gcgggcgttg cgggagctgc gggagctgcg ggcgttgcgg gcgttgcggg cgtttgcgtt
     4141 gcggggagga cggcggtgat gccggcgccg actaggggtc agcgacgaca agcggtgatg
     4201 gcgtggttgt tggcggtgcg gcggcctccg gcgccaccag tgctctttgc aacttggttg
     4261 cacagggtgc tggagggggt tcggggtaga gcctccggtg cctaccccat cgaggtcacg
     4321 gggccccacg cgaacggcgc cccgcatggg ggcgccgctc gctcgacgcg ggtgtgagcc
     4381 cttcagccga cgggtagcac gaccaacgac ggccgcatct cgtcgtgata gagcgaagca
     4441 cgtcgccgcc cctgctcgcg gcctgcggca cgggactccg gcctgccgcc cgttagaatc
     4501 cgccgcgatg cgtcctgtga ccgtgatcct cgcctcggac gagttcgtcg cctccgagtc
     4561 gctcgaccgt ctgcgcgccg aagccgccgc ggaaggcctg gtggccgaag agatcgatcc
     4621 cggggacccg caagcggtgc gctacgcgct cgacacgccg tcgctgttcg gcggtggccg
     4681 cttcgtgctg gtccgcgacg cgcacacctt ggacgaggac gcgcaggacg cgatcgaagc
     4741 ctgggcgaag aacccggccg acggcgtcgt gctggcgctg atggcctcgg gcaccaccgc
     4801 cgcgaagctg tcgaaacgct tcccggccgg cgcgatcatc aaggccgagt cggtcccgcc
     4861 gtggaaggcc gccgaatggg cggtcaagcg cgcgaaggaa aagggtcgcc ggatgagcgg
     4921 cccggccgcc gccgcgctcg tcgagtccgt cggctcggac ctgcgcgacc tggcggccgc
     4981 gatcgaccgg ctcgtcgaaa ccagcaaggc cgccacgatc gagctcgatc aggtccagga
     5041 acgcttcacc ggggtcgagt cgaaggtcta ccagttcgtg gactcggtgt tcgaccgcga
     5101 cctgccgctc gcccgcaagc ggctgcacgg cctgctggac caaggggaga acccgatcgg
     5161 cctgctcgct gcgctggcgc ggcagttgcg cgtgatcgcc tacgtcagcg gcaacgacgg
     5221 gcgccaggcc gccgcgatcg cgaaggatct gggcctcaag ggcgaagggg cggtcaagcg
     5281 ggcgatacgc caggctcgcg gcttcaaccc cgcgcggatc gccaaggcct accgcgtcat
     5341 ggccgacgcc gacctcgcga tgaaaagcga aggcgacgat ccgctggtcc tggaactggt
     5401 ggtcgacgag atcgccgcgc gctaggcgga atgcctaggc ggcggtggcc gacttggcgg
     5461 tgttgaaggc cttctgcagc ttcgacttgt ggttcgcggc gttgttggcg tgcaggacgc
     5521 cggcgctcgc ggccttgtcg taggcacggc agacggtctt gagcgcggca ccagcggcat
     5581 cgaggtcgcc ggcggcgaga gcctcgcgga accggcgggt cagcgtcttg aggcgcgacc
     5641 gggtggacag gttgcgcacg cgggcccgct cgttttggcg gttccgcttg atctgagact
     5701 tgatgttagc catacgagcg ggggagtgta ccagaccgcc gggtccaggc caatccgtga
     5761 aaacgccctg accaggcacg atgggctggg cgatgcggcg cgcgccgagc gcgtccggca
     5821 tcattggagg tggaatgtcc gcctccgagg cgagcccgtg accgaacccc cgcggccggt
     5881 cctgttcgag gacgtcccct acgccgcgcg cgacggcgag atactgctcg ctcgcatcta
     5941 ccgggtcgcc gatgccgggg ccgcgacggc cgtggtcgac gtgcatcccg gcgcatggtc
     6001 ggagggggat cgcacgtggg gccgcgtcta cggcgaggcg ctcgcgcggc gcggcttcgt
     6061 cgtcgtggcc ctggacttcc ggcaagggcc gcgatttgcg catccggccg cgtcggcaga
     6121 cgtggccacc ggcgtgcgct gggttcgcgc ggcgagcgag cccctcggga tcgacccggg
     6181 ccggatcgcg ctgaccggct cgtcgagcgg cggtcatctc gcgatgctcg ccgcttgccg
     6241 gccgaacgcg cccgaacatg cgggcgcgcc gctgcgatgg gaccgatcgg gcgaggttcc
     6301 cggcgagatc gacgcgagcg ttcgctgcgt cgcggccctt tggccgcccg tcgatcccgg
     6361 cgcgcgttac gaatggctgc tcgaacgact gcgaaccgag ccgccggatc aacacgacca
     6421 ctatcgttcg ctcctcgacg gaacaaactc ctacttcggc gaccgcggag tgatgcgcgc
     6481 ggtgagcgtg ccgcgcatcg ttcgggacgg cgaggccacg cacctgccgc ccgtgtgggt
     6541 gtgctatccg gagctcgacc agaacgtgcc ctcgttcatc gtggaggacc tcgaggccgc
     6601 atggcgcgcg gccgggggca cgatctcggt cagcgtctac gaaggcgcgg aacatgcctt
     6661 cggccattgg cccggcgccg ccacggaccg gttcgtcgac gagctggcgg cctttttgcg
     6721 caggttcacc gaaccggggg aagcgaacac gagctcgcgg acgagcgacg ttcgctcgtc
     6781 gtttcgcagc gaggggcgat aggatcgcct cccgatgccc accgaccagc accttcagcg
     6841 cgacttttgc atcgtggccc acgtggacca cggcaagtcg acgcttgccg accgcttgct
     6901 cgaggtcacc catgcggtgg acgaccggca gatgcgcgcg caatacctgg acaagatgga
     6961 cctggagcgc gaacgcggca tcacgatcaa ggccgcggcc gcgcgcatca actacgcggc
     7021 caaggacggc aagacctacg tcctgaacct gattgacact cccgggcacg tcgacttctc
     7081 ctacgaagtc tcgcgcgcct tgaacgcgtg cgacggcgcg gtgctgctgg tggacgcctc
     7141 gcagggcatc gaggcgcaga cgcttgcgaa cctgcacctg gcgatcgagg cggacctgga
     7201 gatcatcccg gtgatcaaca agatcgacct gccggccgcg cagcccgaga agatcgccaa
     7261 cgagctcaag caggtgatcg gctgcagcga cgacgagatc atcttcgtgt cgggcaagac
     7321 cggcgaaggc gttaccgagc tgctcgaggc gatcgtggag aaggtcccgc cgccgtcggg
     7381 cgatccgctc gggccgacgc gcgcgctgat cttcgactcg atgtacgact cgtatcgcgg
     7441 cgtcatcacc tacatccgtg tcgtggacgg cgagatccac aagggcggcc gcgtccggct
     7501 catggcgacc gaggccgagc acgacgtgga agagatcgga ttccttacgc ccgagccggt
     7561 gccggtggac acgctcggcg cgggcgaggt cggctatctg atcaccggca tcaagaacgt
     7621 tcgcgaggcc aaggtcggcg acacggtcac cgacgcgcgg tcacgcgcgc cgcagccgct
     7681 gcccggctat cgcgacgcca agccgatggt cttcgccgga ctgtatcccg cgcaaggcga
     7741 cgactatccg cagctgcgcg acgcgctcga ccggttgcag ctcaacgacg cggcgctggt
     7801 gttcgagccc gagacgtcca aggcgctcgg cttcggcttc cggtgcgggt tcctgggcct
     7861 gctgcacatg gagatcacac aagagcgcct ggagcgcgag ttcgacctcg aactcgtcgt
     7921 gaccgcaccg aacgtcgcct accgcctgtt catgaccgac ggaaccgaag cgggcgtacg
     7981 caacccgagc gatatgcccg acccgtcaaa gatcgagcgg atcgaggagc cgatggttcg
     8041 cgcgatggtg ctgtcgccga ccgagtatgt cggcacgatc atggatctgt gcatgacgcg
     8101 ccgcggcgtg ctggacggca tgcactacct gtccaccgag cgcgtcgagc tgaagtaccg
     8161 tctgccgctg tcggagatca tcttcgactt cttcgaccag ctgaaggcgc gcacgcgtgg
     8221 gttcgcatcg ctggactacg agcctatcgg ctatcaggcc gccgatctgg tgcgcgtgga
     8281 catcctgctg cacggcgata ccgtcgatgc gttcagcgcg atcgtgcatc gcgagaaggc
     8341 ccaggtctac ggccgcaaca tggtcgagcg gctccgcaag ctcatcccgc ggcagatgtt
     8401 cgacgtgccg gtgcaggccg cgatcggcag ccgcatcatc tcgcgcgaga cgatcaaggc
     8461 caagcgcaag gacgttctgt ccaagtgcta cggcggcgac atcacgcgaa agcgcaagct
     8521 gctcgagaag cagaagaagg gcaagcggcg catgaagcag atcggcagcg tcgaggtgcc
     8581 gcaagaggcg ttcatcgccg cgctgcaggt gggggagatc gaggagcccg actgatggct
     8641 catgcggctt tgctggcgcc gcggttcggc atctacgtgc acgtcccgta ctgtctgtcg
     8701 gtctgcccgt attgcgattt caacgtctac gtcggctcgg ctgccgatgc ggatgcgtat
     8761 ctggatgcgg tgctgatcga ggcggacacg cggcgtttcg agttgcgggg ggaagccggg
     8821 accgtgtttc tgggcggcgg gacgccgtcg ctgatctcgc cgccggcttt gcgccggttc
     8881 ttggattcgc tgcggtcgat ccttccggtc tcgcccggcg ccgagatcac gctggaagcg
     8941 aatccggaaa ccgtcacggt ttcgtctctc gagggatatc gcacggccgg agtgaaccgt
     9001 ctgtcgctgg gggtgcagtc gttcgcgccg cacgtgctgt cggcgctcgg acgcacgcat
     9061 acgccgcccg atgcgggcaa cgcggttgcg ttcgcgcggt cggctggctt cgacaacgtg
     9121 tcggtggact tgattttcgg gacggcgggc gagtcgttgg cggattggtc gtccacgctg
     9181 tcgtccgtgg tcgcgtcggg cgtggatcac gtgtcctgct acgggctcac gatcgaggac
     9241 ggcaccgcgt tcggcgccgc ggtcacgcgc ggtgcgttgt cgccgccgga cgaggatgat
     9301 ctggcgtcga agtacgagat ggcgtgcgag atgctgccgc tacggcacta tgagatctcg
     9361 aactggggcg agcccagccg tcacaacctg atctattgga cccagggcga ttatctgggg
     9421 ctcggtgcgg gtgcgcactc gcatcgcgag gggctgcggt cgtggaaccg gcgcttgccg
     9481 cgcgcgtaca tgaccgatcc ggcgcatccg gttgcgggct cggaagagct gggcgatgcg
     9541 ggaaaggcgc gggagtggct gctgctgcgg ctgcggttgg tggacggcgt ggacctggct
     9601 caggcagcaa tcgtgctcga cgtcgatgtt cggtcgctgg ccacgccgat cgtcgaagcg
     9661 gggctcgcgg tgatggatgg cgacgtgatg aagctcacgc cgcgcgggct cctgctggaa
     9721 aacgaaatcg cattgcggct gggggccgcc taaatggccg aagaccgaac gacggatccg
     9781 gggggacgag gcctcggaac gctcgggttc gtgatcgtca acgcatgtgc tgcagcgggg
     9841 ttgttcctga tcggcctctg gaagcaatac ggacagtcac tggacccatg cgtcgaatcg
     9901 gatcgcgtgt gctcgccggg atcggccgga tggttcgtag ccgccggatt tctagccgcc
     9961 ctatcggtcg gtggctacct cgtggtgcgg cgacgccgct aggagtgacc cggggtttgc
    10021 cggggacctt gggttcctgt ccggttctgc tcgattttct cggaatagac agagccgaac
    10081 atgtgttcga gaatcgcggt catgcgaagc tcgttggatc ggatcacggc cgaggtgcgg
    10141 acgctcgtcg acagggtcgc caccgtgccc tcgtcggatg tggccggggt cttggatgcg
    10201 ttcgagcgat tgagccgggt ggtcgcggcc ggccggctcg cggcgcttgc ggcggtggag
    10261 cgatcgcgaa cgtggcgcgg cgaagggcat gcgtcgttcg cgcattgggc ggccgagcgg
    10321 accggcacga cggtggggca cgcgctcggc gcggtgaccg cggcgaaaac gctggcatcg
    10381 ttgccggcga cgcgggaggc gttggcatcc gggacgctgt cggtgcagca ggcaagcgag
    10441 attgcgggcg cggccgcggt ggatccggcg tccgagccgg ggctgcttgg cgcggcggcc
    10501 gaatcgtcgg cgtccgagct acgggaacga tgtcgcatgg tcaaggtcgc ggccgccggc
    10561 aacgacgcct atgagcggat ccgccgcacg cggtatctgc gattccaaag cgatcccgac
    10621 gacgcggtgc gtgtcgaggg gcggttggtg cccgatgcgg cggcggcgct gatgatgtgg
    10681 tgccgcgccg aggcggaccg gcgcgcggcg atcggtcgcc gcgacggcac gatcgagccg
    10741 cacgaagcgc tgatggccga cgcgttgtgc tcactgcccg gcacggtgtc atccggatcc
    10801 gcgccgcgag cgaaagacgc gattgtgcgc tcgaccgtct acgtgcacgt ggatgcggcc
    10861 gcgtgggaac ggggcagcgc gtcgccgggc gaacgctgcg agatcgccgg agtcgggccg
    10921 accaccgtgg cggccgcgcg gcgcttggcg gcgcagccgg gtgggacgct caagatcgcg
    10981 atccgcgaca agggcaacgt ggtcggagtg gtgaacaccg gccggtacgt acccgcacgc
    11041 gtgggtgcgg cgctggaagt gcgcgacgag cggtgcgtcg tcaagggatg cacccggcgc
    11101 cgcgggctgc agcgtgacca catcgtcgag tacggacgcg gcggggagac gtcgctggcc
    11161 aacttgcagc gggtctgcgg ctaccaccac gatctgaaga cgcacatggg ctggaagatc
    11221 gtcggggagc cgccgaattg ccggatcgag ccgcccgcgc cgccctgatg ccggtctgat
    11281 ccggcaccgc ccggatgccg gatcagaccg cgggcatcgc ccgggcgctt ggatgggcgc
    11341 ctagcactca cccgtgtcga gtgctaacct cgaaaatcac tcatgggcaa cccgaacgag
    11401 ccattggccg accgtcaggg agacatcctg gtggcgatcg tgcgcgagta catccgcacg
    11461 gctgatccgg tggggtccaa gcatctggtc gggaagtaca acttgtccgt ttcggcggcg
    11521 acggtacgca acgagatggc ccggctggaa gagctcgggt acctgcacca gccgcatacc
    11581 agcgcggggc gggtcccgac ggacgcgggc taccggttct tcgttgacca tcagcggccg
    11641 gcgcggctcg ggtcgcagca tcgggccgag atgcagtccg cggtcggcgc ggaaccggcg
    11701 gacatggacg agctgatgcg gcgagccagc gaggcgctgt cgcgcgcgac gaacctggcg
    11761 gcggcggcgc tgacgccgcg gctgcgcacc agccggctca agcacttgga tctggcatgg
    11821 ctcggaccgc ggcggctcgt ggtgatcgtg atcgccgatg gcggccgggt cgagaagcgc
    11881 atcgtcgaac tggagagcga cgtatcggaa gcgacgctgg aggccgcggg agacgagctg
    11941 aaccgactgc tctgcggctc gacggttgac ggggccgagc gcgcggcgcg tgagcgttgc
    12001 gaagctgagg ctgcgggcgc gggggatgcg gccgtggccg gcgcggtggc ctcggcgctg
    12061 aaggcgttct tggacgagga tcaagagctg gtcgtcggcg gcgcggcgac gctcgcgggc
    12121 gcgttcggcg gcgaccagga tgcattcccg cgcgtgttcg aggtgatcga acggcgcatc
    12181 gacctgcagc cgctgatgag cgacgcgcag ccgccggcga gcgtgcgcat cggcagcgag
    12241 gtcccggtcg aggatctcca gatgtgcagc ctgatcttcg ccccgtactc gcacggcgcc
    12301 gcggtcggcg tgatcggccc gacacgcatg gagtatccgc aggtgattgc ggccgtgtcg
    12361 atgatggcgc gactgctgaa caacgccgtc caggatctgt cgtagggttc tcgttgatgg
    12421 cggatccgta cgcgatgctc ggggtttcgc gcagcgcgag cccggaggag atcaagaagg
    12481 cgtaccggcg cctcgcgcgg caataccatc cggacacctc ggccgagccc gacgccgaaa
    12541 cgcgcttcaa gcagatcaac gccgcctacg aggtcttgtc cgatcccgag cgccgccagc
    12601 gctacgacat gttcggcgac gacggctcgc gcgccgcgag cgccggttcc ggcgccggcg
    12661 cgaacgccgg cttcggcgac ctcggcgacc tgttcgagtc gttcttcggc ggcggcgggt
    12721 tcggcggcgg cggacgccgc cgcgggccgc gcagcgtcgc cgaacgcggt gcggacatcg
    12781 agacgaccgt ccggctgacc atcgacgagg cggtcttcgg cgtcaagcac gagctcgagg
    12841 tgttcgcggc cctcgcgtgc gaggtctgcc acggcgccgg cgtgcaagag ggcaccggcc
    12901 acgtgcgctg cggcacgtgc gagggaaccg gcgagatccg gcagatgcag cgaagcatct
    12961 tcggcgcgat gatgaccgcg cgcgcctgcg ggcgctgccg cggcaccggc caagtaccgg
    13021 aagcgccgtg cccttcgtgc gcgggcgaag gccggatgat gcgttcgatg atggtcaccg
    13081 tcgacatccc cgccggcgtc gagcacggca ccacgctgcg cgtgcgcggc cgcggtgagg
    13141 ccggcgtgcg cggcggtccg cccggcgacc tgtacgtgca catcggcgtc gagccgaacg
    13201 agacctatca ccgcaagggc gacgatctgc tgtgcgcgct gcgcgttccg ctgacgcagg
    13261 cgttgctcgg cgcgacggtc tcggtcacca cgctcgaagg cgaagaggag ccgatcgagc
    13321 tcgagcccgg cacgcagccc ggagccgtgt atcgcatcaa aggacgcggt gtgcccaagc
    13381 tcggcggccg tggccgcggc gacctcgtca tcgagatcga cgtcgagatc ccgcgcaagc
    13441 tcagcggcga gcaacgcgac ctggtgcgcc gcctggccga gcttcgcggc gagctggtcg
    13501 ccgagaagcc gcgcaaacgc ggcttcctcc ggtgagcgag ctccgccgcg cccgcaccgg
    13561 gaatcggccg atgccttcgt gcttagggga gcgggcggtg aacgggggag cgggcggtga
    13621 acgggtgagc gggcgctgaa cgggtgagcg ggcggtgaac gggtgagcgg gcggtgagcg
    13681 cgcatcattt cttcttgccc tcgatcgagg gtgttctgct gaccatcgaa ggcgaggacg
    13741 cccggcacgc gactcgcgtc ctgcgcatcc gtcccggcga ggcgatcacg gtctccgacg
    13801 gccgcggcgg gatcgcgacc gcgaccgtga ccgacgcctc gcgggcattg agcgcgtcgg
    13861 tcatggaccg tcgcgtggtc gaaccgatcc ggcccgcgct taccgtgtat caggcgctgc
    13921 ccaaagccgg gaagctcgag cgggtggtgc aggcgctcac gcagtgcggc gtcgcggcga
    13981 tcgtgccgtt gcgcgccgct cgcagcatcg cgaagtggga cgcggccaag gcggcatcga
    14041 acgtcggtcg tctgcgcgcc gtcgcgcgcg aggccgcgat gcagtcgcgc cgcgcctggc
    14101 ttcccaccgt ccgcgacccc atcggcatcg acgaggtccc ggccggaacc atcgtcctgc
    14161 acgaagccgc gagcacgcgg ctgtcggcgc tcctgcccct cgcgtccggc gctcctgcgg
    14221 cggtctcaat cgcgatcggg cccgagggtg gattcgacga cgcggagatc gccgcgctcg
    14281 aggcccgcgg ctgccctgcc gcgaccctcg ggccggtcgt gctgcggacc gaaaccgctt
    14341 cgatcgtggc cgccgccgtc attctcgctc gctatggatc gctgggctaa ccgcgcccgg
    14401 tggctgccgg tttctccgct tttgtcgaga cattcgcggc ctgtgactag gactttttag
    14461 ggcaaaccga ttgtcagcgg ccacagcgcg gcagtaggct tcgcgggagc agcggtgacg
    14521 ctgcgtggca acgcacgtcc agggtgccgg cgcgtggagg gtccgatggc cgacgacggg
    14581 gtgaattcga cgtacgcaaa gcgagtcggt gagcgcctgc gcgccattcg tcttcagaag
    14641 ggctactcgc tccacgacgt cgaggacgtc tcgtccaagg agttcaaggc ctcggtgctc
    14701 ggcgcctacg agcgcggcga gcgcagcatc tcggtcccgc gcctgcagcg gctcgcacag
    14761 ttctacggcg tgcccgtcga tcagctcctg cccaagctcg aaggcgagac gatcgtgctc
    14821 gacggacgcg gcccgcagga aggcggcatc cggctcgatc tcacgaagct ccgcgagacc
    14881 aacggcgccg agaccgacgc ggtctcgcgc tacgtcacca tgatccagat ggcccgcggc
    14941 gacttcaacg gccgcgtgct caccgtgcgc actgaggacc ttcgggcgct cgccgccgcg
    15001 ctcgatctga cgccgcaagc gctcaagagc cggctcgacg aactcggcgt ccgtatcccc
    15061 gcgctcgtct aacgcgcctg ccgcgatagg ctcgcgccca tgcgtgcctt ggtgacgggc
    15121 gcaaccggct tcatcggcgg ccggctcgtc ccgcgtctcg tcgaacgtgg ccaccgcgtc
    15181 accgcgctcg ttcgatcacc cgatcgtgcg gccgggctgc gcgagatggg cgtgacgctc
    15241 gccggcggcg acatcaccga gcccgcgacg ctcgaaggac ctatgcgcaa ggccgacgcc
    15301 gttttccacc ttgcggcctg gtacgagatg ggcgtaggcg accgcacgaa gatgtatcag
    15361 gtgaacgtgc gcggcaccga gcacgtgctc gcggccgcgc gcgaagccgg cgtcgggcgc
    15421 atcgtgcact gctcgacggt gggcgcgctc ggcgccgggc cgatcggcga catccgcagc
    15481 gagacctcgc agcattcggg ggcattcggc tcggtctacg aagagacgaa atggcaggcg
    15541 catcaggttg cgcggcgcgc ggccgccgac ggcgcgccgg tcgtgatcgt gatgccgggc
    15601 gcggtctacg gacccggcga tccgagcgtg ctcggggcga tgatccgcct ctacgcgaag
    15661 ggctggctga tcgcgttgcc gttccaggac agcggctttt cgtgggtgca cgtggacgac
    15721 gtggccgaag gcatcgtgct cgcccatgag aagggccgcg ccggcgagga gtacgtgatc
    15781 ggcggcgcga acgcgaccgt cggcgacgtg ttccgacagc tcgcgccgct gaccggtatc
    15841 cgtgcgcctc gcttcgagct tcccggatgg atgctgcgcg cgtcgatgcc gctgtccccg
    15901 gtcatcgccc gcaccctcaa cgccggcccg cgtatcgtgc aggacgcgct gtcgtcgatg
    15961 cgcggctcgt tcatggcctt ctccaccaag gccgaagccg agctgggcta tcgccaccgg
    16021 agcatcgaag agggaatgcc cgagaccgtc gaatggttca aggagaaccg ctgagcgaag
    16081 acgttcagat gctcgacgtt cgggcatgag agcgaaccgc tgaggagggc gccatggagg
    16141 attgcttgtt ctgcaagttc gtctcgggcg agatcgcgcc cgacgtggtg taccaggacg
    16201 atctcgtgct cgcgttccgc gacatcaatc cgcaggcgcc gacgcacgtg ctgatcgtgc
    16261 ccaagcagca caccaaggac gcgtccaccg tcgagccgtc cgacgggccg gcgctcgccg
    16321 cgatcacgaa cgccgcccgc accatcgccg cgcaagaagg gctgccgggc ggctatcgcc
    16381 tggtcgccaa cgtcggcccc gacgccggcc agagcgtatt tcacctccac gtccacatgc
    16441 tcggcggccg ctccctcgcc tggccccccg gataatcgac cagacgggcg atcagacggg
    16501 cgatcagacg ggcgatcaga cggacgaatc gggcggatct gttgcggccg ggcggccgcc
    16561 gcgccgatat aattccggga cccgaccaac ggagacggat tgagcaggaa agacaccgag
    16621 gaacggcgca ccaccacgca ggtcaagatc ttggttccgg gccatcatgc gatggtcggc
    16681 ctggtgggcg agcgcgacga gttgctcaag gtcgtcgaag ccgcgttcga ggctcaaatc
    16741 ctggtccgcg gcaacgagat caccatttcc ggggagcccg gccacgtcga gcgcgtctcc
    16801 acgctgttcg aggagctgat cgagctgctc gagcgcggca ccgtgctgac cgccgacagc
    16861 ctgacccgtt cgatcgacat ggtcaaaggc atcgcgggcg agacggcgcg gcccagccag
    16921 gtgctgtcgg acgcggtgct cacgcatcgc ggaaaggcga tcaagcccaa gacgctcggg
    16981 caaaagaagt acgtggacgc gatccgaaag tccacgattg tgttcggcat cggccccgcc
    17041 ggaactggca agacctacct cgcgatggcg atggcggtga aggcgctgca agaaaagcag
    17101 gtcgcgcgcc tgatcctgac ccggcccgcg gtcgaggccg gcgaaaagct cggcttcctg
    17161 cccgggacgc tctacgagaa gatcgatccg tacatgcggc cgctctacga cgcgctctac
    17221 gacatgatgg accccgaatc gctgtcgcgg ctcacgcaac gcggaacgat cgaggtttcg
    17281 ccgctggcct tcatgcgcgg ccggacgctc aacgactcgt tcatcatcct ggacgaggcg
    17341 cagaacacca cgcccgagca gatgaagatg ttcctgaccc ggctcggctt cggctccaag
    17401 gtcgtggtga cgggcgacgt gtcccagatc gaccttccgt ccgggcagtc cagcggactg
    17461 aacgtcgttc gggagatcct caccgagatc gacgggctcg agttcgtctt cctgggcagc
    17521 aaggacgtgg tcaggcactc gatcgtccag gagatcgtcg aagcgtaccg gcgtttcgac
    17581 gaggcccact aggccgaaac tagacggagg ccccaggcct tgctaaaacc aagtcaaatc
    17641 ccatgagcga ccccgacgat cccggcggac cgggaagtct tcccggcggc cccgttacgg
    17701 tggccctcgc cgacgagcaa accattcccg tcgatcccga tgcgctggtg catgccgcgc
    17761 gccgcacgat ggacatcctc ggcatgcgcg gcgagctttc gctcgcgctc gtggatccgg
    17821 cccggatcgc ggggctcaag cgcgactact acggcgaggc cgccgcgacc gacgtgctgt
    17881 cgttcccgat ggacggcgcc ggcgggccga tgatcggcga cgtgatcatc tgtcccgcgg
    17941 tcgcactgcg ccaggcgcgc ggcatgcacc agcgcatcgg gatcgacggg aatggtttgc
    18001 tcgtcggcga gggccaccgt aacggggccg ccgggaagac ttcccggtcc gccgggatcg
    18061 tcggggtcgc tcatggg
//
LOCUS       DATLFO010000316         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7611, whole genome shotgun sequence.
ACCESSION   DATLFO010000316 DATLFO010000000
VERSION     DATLFO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7611"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VM841_12860"
     CDS             complement(<1..373)
                     /locus_tag="VM841_12860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HVL91115.1"
                     /translation="MRDPGFFGPDSVSWQVHRETTVLFGGARALLMQAAHPLVIAGAS
                     ATGFYERNPWARLMRTLHLTYAITFASKDEALAAAERINDVHRRVHGVDPITGKHYDA
                     LDPELLLWVHAVLVDSALEFER"
     gene            536..1546
                     /locus_tag="VM841_12865"
     CDS             536..1546
                     /locus_tag="VM841_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91116.1"
                     /translation="MAERMSRAAMIAAGIALLAATFGYLLFWRTVPFEAMTADFRPLM
                     TARSVAQIEADVQGLEAAAAEYRTALIPTITARTGSSVDALRARLARQYPAVTSGMDQ
                     IPRIAAGMRSLAATFESERVRFAAADAIPSKGITTTAIPWLLTIAAAIAIVAGLSAAG
                     SRGTAIAAVVAGVMLAAAPLLLSMPSKAAAADRLNDNLAPLFNTGRIVQTEQAVDILQ
                     AMAAELTTTMLPQISARLGLPPEQITPFFAQNFPALTAMVQTAPEALARAEAIAHVHA
                     GNQARYEDIRPVSFRVLTWTSLGAGLTLIVAGSVASGRAARIAAFEPGEGRRPGRRRV
                     AA"
     gene            1767..2324
                     /locus_tag="VM841_12870"
     CDS             1767..2324
                     /locus_tag="VM841_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91117.1"
                     /translation="MTTQTESRRVAVLIGGGAALAFLGLLLPWVSVQLSIGAESTSLS
                     QSGLATDDGKVILIIAVLLGLIAAPFFTGRIRRVLIAGIVALLLSTVVTLVGFADIND
                     IRSRGDETEAVSATVGFGLYVTTAGGLVALMGSFMAIRGRRTLGEAAAPGGDDDAIPP
                     APPAPPPAETPTLPSEQPPPPPPAP"
BASE COUNT          348 a          841 c          836 g          327 t
ORIGIN      
        1 gccgctcgaa ctcgagcgcc gagtccacga gcacggcgtg cacccacagc agaagctccg
       61 ggtcgagcgc atcgtagtgc ttgccggtga tcggatcgac gccgtgcacc cggcggtgta
      121 cgtcgttgat gcgctcggcc gcagcgagcg cctcgtcctt ggacgcgaag gtgatcgcgt
      181 aggtcagatg gagcgtgcgc atcaggcgcg cccaggggtt gcgctcgtag aagcccgtcg
      241 cgctcgctcc ggcgatcacg agggggtgcg cggcctgcat gagcagggcg cgggcaccgc
      301 cgaacagaac ggtcgtctcg cggtgcacct gccacgagac gctgtccggc ccgaagaacc
      361 ctggatcccg cacgcccgcc aagcgtagcc gggcggcgcc gcatcacgcg acgattcggc
      421 gcaaacgggc gggctagtcc cggcggaccc ccgatacgcc cgttcgggct atcgacgctg
      481 atccgatcgc acgacaggct tgagcggggc gggaggctcg aggagaggac gcgtcatggc
      541 cgagcggatg agccgcgccg cgatgatcgc cgccggcatc gcgctcctcg cggccacgtt
      601 cgggtatctg ctgttctggc gaaccgtgcc gttcgaggcg atgaccgcgg actttcggcc
      661 cttgatgacg gcgcgcagcg tcgctcagat cgaagccgac gttcaagggc tcgaggcggc
      721 ggcggccgag taccgcacgg cgctgatccc gacgatcacg gcgcgaaccg gctcgtccgt
      781 ggacgcgctt cgcgcccggc tcgcccggca atatcccgca gtgaccagcg ggatggacca
      841 gattccccgg atcgccgcgg gcatgcgctc gctggccgcc acgttcgagt ccgagcgcgt
      901 ccgtttcgcc gccgccgacg cgatcccgtc caagggaatc accacgaccg cgatcccatg
      961 gctgctcacg atcgcggccg cgatcgcgat cgtcgccgga ctgtcggccg ccggcagccg
     1021 cggcaccgcg atcgcggccg tggtcgccgg ggtgatgctc gccgcggcgc ccctcttatt
     1081 gtcgatgccg tcgaaggccg ccgccgcgga ccggctcaac gacaacctag cgccgctgtt
     1141 caacaccgga cgcatcgtgc aaaccgagca ggcggtcgac atcctgcaag ccatggccgc
     1201 ggagctcacc acgacgatgc tgccgcagat ctcggcgcgg ctcggcctgc cacccgagca
     1261 gatcacgccg ttcttcgcgc agaacttccc ggcgctgacg gcgatggtgc agaccgcgcc
     1321 cgaggccctg gcgcgcgccg aggcgatcgc ccacgtccac gccggcaatc aggcgcgcta
     1381 cgaggacatt cgcccggtgt cgttccgggt gctgacctgg acctcgctcg gcgcggggct
     1441 gacgctgatc gtagccggct cggtcgcctc cggccgcgcg gcgcgcatcg ccgcgttcga
     1501 gcccggtgaa gggcggcgcc ccggacggcg tcgcgtggcg gcgtaggcgc gggctcgaaa
     1561 taggagcgtt gcacatgatc gccggggccg agcgcggagt gcatcggatg gatgatcgcg
     1621 aggcggcggc agaagctcca ggaaacgcgg cagcccgaag cgccagagga acacgtcgtg
     1681 gccgggcgtg cgtccttgtg ccgggcatgc gcgaccgata cggtcaggcg cacgaccgag
     1741 gaagcgaacg caaagggggc ggaacgatga ccacgcagac cgaatcgcgg cgcgttgccg
     1801 tgctgatcgg cggcggcgcg gccctggcgt tcttggggct gctgcttccg tgggtgagcg
     1861 tccagctgtc gatcggcgcg gagtcgacct cgctcagcca gtcggggctc gcgaccgacg
     1921 acggcaaggt gatcctgatc atcgcggtcc tgctcggcct gatcgcggcg ccgttcttca
     1981 ccggccgcat ccggcgcgtc ctcatcgccg gcatcgtcgc gctgctgctg tccaccgtgg
     2041 tcacgctcgt gggcttcgcg gacatcaacg acatccgcag ccgtggcgac gaaaccgaag
     2101 cggtctcggc caccgtcggg ttcgggctct acgtcacgac cgcgggcgga ctcgtcgcgc
     2161 tgatgggatc gttcatggcg atccggggcc gccgcacgct cggcgaggcc gcagcacccg
     2221 gcggggacga cgacgcgatt ccgcccgcgc ctccggcccc gcccccggcc gagacgccga
     2281 cgctgccgtc ggagcagcca cctcctccgc cgccggctcc ctagcccagc cggtagaagc
     2341 gaaacatctc gg
//
LOCUS       DATLFO010000317         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_77784, whole genome shotgun sequence.
ACCESSION   DATLFO010000317 DATLFO010000000
VERSION     DATLFO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77784"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="artA"
                     /locus_tag="VM841_12875"
     CDS             <1..622
                     /gene="artA"
                     /locus_tag="VM841_12875"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HVL91118.1"
                     /translation="FVTCAVYFSVLRIKPIADWLILTVANQTVWLLQLFNQNVIIEGA
                     SICYAPGPCDGYNGPTVNIILACTAIQSMMIFVGALAVVPNASMKARTLGLLATVPPI
                     YALNLVRNASIIWMWAAEGWSFSFAHNVVGKGGSLLALVALAFITFQIVPQLYDAIVG
                     LLDLPKRRGPLEVYWNSRKGPPKQPAQTPAPTPSLAANEPTPHESK"
     gene            1560..2303
                     /locus_tag="VM841_12880"
     CDS             1560..2303
                     /locus_tag="VM841_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91119.1"
                     /translation="MKVRPGALLALLVMLLPAFTAATPQQGVIAPGIQIRSVEDGWTC
                     TGNFLFESRVNASLYLGIADHCLNKSDVGDPVLIGQDGATGRVAYSARRVMQARGEEL
                     VANDFGLIEIDKPFRGWATASVLYYGGPTSLAACSSLRDGDRILVYGSSPWHLRLDAL
                     QRMEGYAHLLAPPYNFDILMRTPAVWGDSGAPALTADGRAIGVVVALSATNLAVCLDR
                     VFEYARTAGGLDIQLLTAAQRNAGSLPDF"
     gene            complement(2347..>2578)
                     /locus_tag="VM841_12885"
     CDS             complement(2347..>2578)
                     /locus_tag="VM841_12885"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HVL91120.1"
                     /translation="WLLGWIPPLIPFTVIMLILAIIFGAIANGRANDDPAVGGKGLAI
                     AGLVLGILGLVTYLLVVLFMVLFFAAIFGSIF"
BASE COUNT          452 a          848 c          792 g          486 t
ORIGIN      
        1 cttcgtcacc tgcgccgtct acttctccgt cctgcgcatc aagccgatcg cggactggct
       61 cattctcacg gtcgcgaacc agaccgtgtg gctcctccag ctgttcaacc agaacgtcat
      121 catcgagggc gcgagcatct gctacgcccc cggcccgtgc gacggctaca acggccccac
      181 ggtgaacatc atcctggcgt gcaccgcgat ccagagcatg atgattttcg tcggcgccct
      241 cgccgtcgtg ccgaacgcgt cgatgaaggc gcgcaccctc ggcctcctcg ccacggtgcc
      301 gcccatctac gcgctgaacc tcgtgcgcaa cgcgagcatc atctggatgt gggccgcgga
      361 ggggtggagc ttcagcttcg cgcacaacgt cgtcggcaag ggcggcagcc tgctcgccct
      421 cgtcgcgctc gcgttcatca cgttccagat cgtgccgcag ctctacgacg cgatcgtcgg
      481 cctgctcgac ctgccgaagc gccgcgggcc gctcgaggtc tactggaact cgcggaaggg
      541 gccgccgaag cagccggcac aaacgcctgc gccgacgccc agcctcgccg cgaacgagcc
      601 gacgccgcac gagtcgaagt agtactcgcc gcgtcctcct caacggccgc gcggtgcggc
      661 cgccgcgagc gttcgttttt tttggtcaag ctttttcccg gaaggccgcg cgcagcgcgg
      721 ccactccgag aaaaaggttg cagcgagcga gccgacgctg cacgagtcga agtaaccgcg
      781 tttgtgactg gggaagtcat ttcgccccgg tgattgcgat cttcggctgt tccagaaaag
      841 atgcccctga gatctgaatc cggcaaattt ctcgaacata ttcgccttct tcctacttga
      901 ggtactcatt tatttcagaa acgcctgccg ggattgaagg tgcgcagcaa tgtccctcca
      961 agcctccctc gtggctttca gcttcatctt gattccgatt gccatgttgg cacattcggc
     1021 gacagcgttg ggccctgagt gcttggaagg acccaacgtg cctgaggatt gtgtccccga
     1081 ggatatcgac gacgtagtca ctgtggagcc catcggtgcg acagcttgca tctcgcctca
     1141 gacgtgcctg ggccctcaga ccttgcttcg gccggtcata tcaggcacca ttccgaaccc
     1201 ggtcccagag caggtcgtct atatcacccc gtgtcttgtc ccgggggacc ctatgccatc
     1261 ggtactcctg gttctggtga cccttgatgg catttgtccc atcggtgacc cacacccgga
     1321 gctcaaccca attccggcaa gcacatgcac catcggagaa gcatgcaacg atctgcccaa
     1381 cgctggcttc gagaacttcg tgaccccgga cccgcccaat ttctgtgcga cgccctcccg
     1441 cgtaaaggtc ttgggggtta cggtcttgag cagcattccc gtcgtgagcg tggatgggtg
     1501 ctagaacccg cttaagcccg cgccacaagg gctcggtgcc ggattctgtt ccatatcgat
     1561 tgaaggtgcg gccgggagcg ttgctggcgc tattggtgat gctgctgcca gccttcaccg
     1621 cggccacgcc gcagcagggc gtcatcgctc cgggcatcca gatccgttcg gtcgaagacg
     1681 gttggacctg caccgggaac tttctgttcg agtcgcgtgt caacgccagc ttgtatctgg
     1741 gcatcgccga ccactgcctt aacaagagcg acgtaggaga cccggtcctg attggacagg
     1801 acggggcgac gggccgcgtg gcgtattccg cccgacgcgt gatgcaggcg cgcggcgagg
     1861 agcttgtggc aaacgacttt ggcctcatcg agatcgacaa gcccttccgc ggttgggcaa
     1921 cggcctccgt gctctactac ggtggaccga cgtccctcgc ggcctgctcc agcttgaggg
     1981 acggcgaccg cattctcgtg tacggctcga gcccctggca cttgaggctc gacgccctgc
     2041 aacggatgga aggctacgcg cacctgctcg cccccccgta caacttcgac atcctgatgc
     2101 gcactccggc ggtgtggggc gacagcggcg cgccggccct gacggcggac gggagggcaa
     2161 tcggcgtggt ggtcgcactc tctgcaacga atctggccgt ctgcctcgac cgggtcttcg
     2221 agtacgccag aactgcgggg ggactggata tccagcttct gacggcggcg caacgaaacg
     2281 cgggctcgct tcccgacttt tagtggagga gccccttgcc cggaggattc cgcgcctgtt
     2341 ggggcctcaa aagatcgacc cgaagatggc ggcgaagaac agcaccatga acagcacgac
     2401 gagcaggtag gtcacgagcc cgaggatgcc cagcaccagt cccgcgatgg cgagtccctt
     2461 gccgccgacc gcggggtcgt cgttcgcgcg accgttcgcg atggcgccga agatgatcgc
     2521 gaggatcagc atgatgacgg tgaacgggat gagcgggggg atccacccga gcagccag
//
LOCUS       DATLFO010000318         6427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_137084, whole genome shotgun sequence.
ACCESSION   DATLFO010000318 DATLFO010000000
VERSION     DATLFO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137084"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="carB"
                     /locus_tag="VM841_12890"
     CDS             complement(<1..815)
                     /gene="carB"
                     /locus_tag="VM841_12890"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141767.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HVL91121.1"
                     /translation="MPRRDDIEKILIIGSGPIVIGQACEFDYSGTQACRVLREDGYQV
                     VLVNSNPATIMTDPEFADRTYIEPITPAFVERVIERERPDALLPTLGGQTGLNVAVEL
                     AESGVLEKYDVELIGARLDAIRRGEDRKLFRETMQSIGLDLPRSTHAYTVEEALATAN
                     EIGYPVILRAAYTLGGAGSGVVWNDHELEKLAREGLSASRIGEILVEESVLGWKEYEL
                     EVMRDLADNVVIICSIENVDPMGVHTGDSVTVAPAQTLSDREYQTMRDAAIRC"
     gene            complement(816..1175)
                     /locus_tag="VM841_12895"
     CDS             complement(816..1175)
                     /locus_tag="VM841_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91122.1"
                     /translation="MTERVAVKGLRTLGAPPKAKRSAAPKRNKTQQAGSDMAKKVLQA
                     EFRKALKLKEKLAGEQAALSDDKGRDVREAEKLRSIVASLEGMSRFAIVMGLLTPEEN
                     RALWANAMKKGLYEGWR"
     gene            complement(1172..2329)
                     /gene="carA"
                     /locus_tag="VM841_12900"
     CDS             complement(1172..2329)
                     /gene="carA"
                     /locus_tag="VM841_12900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HVL91123.1"
                     /translation="MSKAILVLEDGTTFPGRSFGAPGETFGEVVFNTSLSGYQEVLTD
                     PSYYGQIVCMTYPHIGSYGVTPEDVESGRIQVSGFAVRDAVREYSNWRATQSIHEYLE
                     EAGITAIEEIDTRKLTRHIRSLGAMRGAISTDDLDPASLRGRVLASPSMEGLNLVDPV
                     TTAQPYRTEDVLGIKPSPRQDGRIFRVAAYDYGIKFNILRLLAAHGCDVTVYPAAHPA
                     ADLLAAGYDGVFLSNGPGDPASVRVAIDNAKTLLGNVPVFGICLGNQILGLALGGGTY
                     KLKFGHRGINQPVKRLATGAVEITSHNHGFAVDADSFPRDGDDSLKTEFGRAEISHVN
                     LNDGCVEGLRCLDVPAFSVQYHPEAAPGPHDARYLFEEFATLMANPEEGQA"
     gene            complement(2439..3743)
                     /locus_tag="VM841_12905"
     CDS             complement(2439..3743)
                     /locus_tag="VM841_12905"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVL91124.1"
                     /translation="MAEPILIKGGRLVDPAAGRDVVLDVRIDGGHVAEIGPDLSADGG
                     LVVDAAGLVVAPGFVDLHTHLREPGREDAETIESGTRAAVLGGYTAVSAMANTDPIAD
                     SAGIVEQVWNLGRAAGWCDVHPVGAITAGLRGEMLAEIGEMAASTAKVSFFSDDGTCV
                     NDAALMRRALEYARAFDAVVANHAEEGALAEGWSMNEGEVSALLGMRGLPAEAEEIIV
                     ARDIMLARLTGGRLHVPHVSTAGTVELIRAAKARGIPVTAEVTPHHLSLTEDSVRSFD
                     PVYKVAPPLRTKADVEAVREALADGTIDCVATDHAPHTLESKDVEFDLAPCGMIGLET
                     ALSVVLTDTSLDLTAVVERMSVAPARIRRLKDQGGPIAEGSVANLVVFDPDATWTVNP
                     HGMASKSRNTPFAGRELKGKVLWTIYRGRVVVSEGRIDHGKR"
     gene            complement(3736..4677)
                     /locus_tag="VM841_12910"
     CDS             complement(3736..4677)
                     /locus_tag="VM841_12910"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVL91125.1"
                     /translation="MIKHLLDITDLDRDGIERILETAANLQPDGGAIRKYPALRNRTV
                     VNLFYESSTRTRTSFELAAKRLSADVVNFAVSGSAVEKGESLKDTAQTIMAMGVDAIV
                     IRHKASGAPHRLAQWVDAHVINAGDGMHQHPTQALLDLFTIRQHFPSFEGLRVAIVGD
                     VTHSRVARSDVQALATMGAEVTLVGPPTLIPPQAEHWGVTVTTDLDAVLPKADVVYLL
                     RMQLERQKAGYVPSLREYSRLWGLDSARASMLSDEALVMHPGPMNRGVEISAEVAELP
                     RSLIVDQVTNGIKVRMALLYLMLGSPDGSAEGAKANG"
     gene            complement(4674..5237)
                     /gene="pyrR"
                     /locus_tag="VM841_12915"
     CDS             complement(4674..5237)
                     /gene="pyrR"
                     /locus_tag="VM841_12915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HVL91126.1"
                     /translation="MGEASPLSGKPKAAPMDAGDLSRALARIAHEILERNKGAEHLVL
                     VGLLSRGEIIAQRLAERIADIEGVKVPTGSLDIGLYRDDIGLRAPAELKTDHIPPIDD
                     KIVVLVDDVLYTGRSVRAALDALMDVGRPKAVQLAVLVDRGHRELPIRADYVGKNIPT
                     AHGEEIVVRVAEADGEDAVLVAEREES"
     gene            complement(5443..>6427)
                     /locus_tag="VM841_12920"
     CDS             complement(5443..>6427)
                     /locus_tag="VM841_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91127.1"
                     /translation="GCHDVRFRGDRVYCAAVNTTLILDISGMVGADGRLTGTKLTDNN
                     GCPVIDAVRAPGVKITDCMAWTPEQFIAAKARPMDIRLVSWIQHDNTKPAEQDIEIAH
                     QAEVIGDGTTMLITDERGGGLANVQGCPGGGIWFYDIRDETKPKLMKRSDGSPGMFRT
                     ASSPPPPFGQSVSCTSHYGQEFADENLLAFAWYHAGTSVFRYTLDLTTSPATIGFEEV
                     ASTVPAGSMAIQAWAAGRNPDDPGEVIVYVSDAVRGLDVLGVRTPKVTRAKAFKQASA
                     EPAPKPAPKPAVVRGARTALPATGVGSAIPAGLVAVSGAAALASTLRRRKH"
BASE COUNT          937 a         2152 c         2233 g         1105 t
ORIGIN      
        1 atgcaacgga tcgcggcgtc gcgcatcgtc tgatactcgc ggtccgacag ggtctgcgcg
       61 ggcgcgaccg tcaccgagtc gccggtgtgc acacccatgg ggtcgacgtt ctcgatcgag
      121 cagatgatca caacgttgtc agccaggtcg cgcatgacct cgagctcgta ttccttccag
      181 ccgagcaccg actcctcgac caggatctcg ccgatgcgag aggcgctcag gccttcgcgc
      241 gcgagctttt cgagctcgtg atcgttccag accacgccgg aacccgcgcc accgagggtg
      301 taggcagcac gcaggatcac cggatagccg atctcgttcg cggtggcgag tgcctcctcg
      361 acggtgtagg cgtgcgtgct gcgcggcagg tcgaggccaa tcgactgcat ggtctcgcgg
      421 aacagcttgc ggtcctcgcc gcggcggatc gcgtccaggc gggcgccgat cagctcgacg
      481 tcgtactttt cgagcacgcc cgactcggcc agctccaccg cgacgttgag gccggtctgc
      541 ccgccgagcg tcggcagcag cgcgtcggga cgctctcgtt cgatcacgcg ctcgacgaag
      601 gcgggcgtga tcggctcgat ataggtccgg tcggcgaact cggggtcggt catgatcgtg
      661 gccgggttcg agttgaccag cacgacctga tagccgtcct cacgcagaac gcggcacgcc
      721 tgcgtgcccg agtaatcgaa ctcgcatgcc tgcccgatta cgatcgggcc ggacccgatg
      781 atcaggatct tttcgatatc gtcacgtctg ggcacttatc gccatccctc gtagaggccc
      841 ttcttcatcg cgttcgccca cagggcgcgg ttttcttcgg gcgtgagcaa tcccatcacg
      901 atcgcgaagc ggctcatgcc ttccagcgac gccacgatcg agcggagctt ttcggcttcg
      961 cgcacgtcgc ggcccttgtc gtcgctgagc gcggcctgct cgccggccag tttctccttt
     1021 agcttgagcg ccttgcggaa ctcggcctga agcaccttct tggccatgtc cgagccggct
     1081 tgttgcgtct tgttgcgctt gggcgcggcc gagcgtttcg ccttcggcgg cgcgccgagc
     1141 gtgcggagcc ctttgactgc gactcgctcc gtcatgcctg accctcctct gggttggcca
     1201 tcagcgtcgc gaactcttcg aacaggtacc gggcgtcgtg cggtcccggc gcggcctccg
     1261 ggtggtactg cacgctgaac gcgggcacgt ccaggcagcg aagcccttcg acgcagccgt
     1321 cgttcagatt gacgtgcgag atctccgcgc ggccgaactc ggtcttgagg gagtcgtcgc
     1381 cgtcgcgggg gaacgagtcg gcgtcgaccg cgaaaccgtg gttgtggctg gtgatctcga
     1441 ccgcgccggt cgccaggcgc ttgaccggct ggttgatgcc gcgatgcccg aacttgagct
     1501 tgtaggtgcc accgccgagc gcgaggccca ggatctggtt gccgagacag atcccgaaca
     1561 ccggcacgtt gccgagcagc gtcttggcgt tgtcgatcgc cacgcgcacc gaggccggat
     1621 cgcccgggcc gttcgacaga aacaccccgt cgtagcccgc ggccaggagg tcggccgccg
     1681 ggtgcgcggc gggatacacg gttacgtcgc agccgtgcgc ggccagcaac cgcaggatgt
     1741 tgaatttgat gccgtagtcg taggccgcga ccctgaagat gcggccgtcc tggcgcgggg
     1801 acggcttgat gcccagcacg tcctcggtgc gatacggctg cgcggtggtc accggatcga
     1861 cgaggttgag gccttccatc gacggcgagg cgagcacgcg cccgcgcagc gacgccggat
     1921 cgaggtcgtc ggtcgagatc gcgccgcgca tcgcgcccag cgaccggatg tggcgcgtca
     1981 gcttgcgcgt gtcgatctcc tcgatggccg tgatcccggc ctcctcgagg tactcgtgga
     2041 tcgactgggt cgcgcgccag ttcgagtatt cgcgcaccgc atcgcgcacc gcgaagcccg
     2101 acacctggat ccggccggac tcgacgtcct cgggcgtgac gccgtagctg ccgatgtgcg
     2161 gataggtcat gcacacgatc tggccgtaat acgaggggtc ggtcaacacc tcttggtagc
     2221 ccgacagcga cgtgttgaac acgacctcgc cgaacgtctc gccgggcgcg ccgaagctcc
     2281 ggccggggaa cgtcgtgccg tcctcgagaa ccaggatcgc tttgctcatc gtctagcgcg
     2341 agcctgtctt gcgggcctgt ccgcgctcga gcgcttgacg cttgaggcgt tccttcttcg
     2401 cgcgggtgcg gtccttcgcc cagcgcactc gcttgcggct accttttgcc atggtcgatc
     2461 ctcccttcgg agacgacgac gcgtccccgg tagatggtcc atagaacctt gcctttgagc
     2521 tcacggcccg cgaacggggt gttgcggctc ttggaggcca tcccgtgcgg gttgaccgtc
     2581 caggttgcgt ccgggtcgaa cacgaccagg ttcgccaccg atccttccgc gatcggaccg
     2641 ccctggtcct tcaagcgccg gatccgggcg ggcgcgacgc tcatgcgctc gacgaccgcc
     2701 gtcaggtcca gcgacgtgtc ggtcagcacg accgacagcg ccgtctccaa tccgatcatg
     2761 ccgcagggcg ccaggtcgaa ctcgacgtcc ttggactcga gcgtgtgcgg cgcatgatcg
     2821 gtggcgacgc agtcgatggt gccgtcggcg agcgcttcgc gaaccgcctc gacgtccgcc
     2881 ttggtgcgaa gcggcggcgc gaccttgtag acagggtcga acgagcgcac ggagtcctcg
     2941 gtgagtgaca gatgatgcgg cgtgacctcg gcggtgaccg ggatgccgcg ggccttcgcc
     3001 gcgcggatca gctcgacggt gccggcggtg gacacgtgcg gcacatgcag ccggccgccg
     3061 gtcagccgcg cgagcatgat gtcgcgcgcg acgatgatct cctcggcctc ggcgggaagc
     3121 ccgcgcatgc ccagcagcgc gctgacctct ccttcgttca tcgaccagcc ttcggccagc
     3181 gcgccttcct cggcgtgatt ggccacgacc gcgtcgaagg cccgcgcgta ctcgagcgcg
     3241 cgccgcatca gcgcggcgtc gttgacgcac gtgccgtcgt cggagaagaa gctcaccttc
     3301 gccgtcgatg ccgccatctc gccgatctcg gcgagcatct cgccccgaag gccggcggtg
     3361 atcgctccga ccggatgcac gtcgcaccag ccggcggcgc ggccgaggtt ccagacctgc
     3421 tcgacgatgc cggcggagtc ggcgatcggg tcggtgttgg ccatcgcgct gacggcggtg
     3481 tagccgccga gcacggcggc ccgcgtgccg gattcgatgg tctcggcgtc ctcacggccc
     3541 ggctcgcgaa ggtgggtgtg cagatccacg aagcccggag cgaccacgag cccggcggcg
     3601 tcgacgacga gtccgccgtc ggcggaaaga tcggggccga tctcggcgac gtggccgccg
     3661 tcgatccgca cgtccaggac gacgtcgcgt ccggcggccg gatcgacgag ccggccgccc
     3721 ttgatcagga tcggctcagc cattcgcctt cgctccttcc gcggatccat cgggggatcc
     3781 gagcatgaga tagagcagcg ccatccggac cttgatgccg ttggtgacct gatcgacgat
     3841 gagcgagcgg ggaagctcgg cgacctcggc gctaatctcg acgccgcggt tcatcggacc
     3901 gggatgcatg accagcgcct cgtcgctcag catggatgcg cgagcgctgt ccaggcccca
     3961 cagccgcgag tactcgcgca gtgagggcac gtacccggcc ttctgtcgct cgagctgcat
     4021 gcgaagcaga tacacgacat cggccttggg caggaccgcg tcgaggtcgg tggtgacggt
     4081 cacaccccaa tgctcggcct gcggcgggat cagcgtcggc gggccgacca gcgtcacctc
     4141 ggcgcccatc gtggccagtg cctgcacgtc gcttcgcgcg acgcggctgt gcgtgacgtc
     4201 gccgacgatc gcgacgcgaa ggccttcgaa ggacgggaag tgctggcgga tcgtgaacag
     4261 gtcgagcagc gcctgcgtcg gatgctggtg catgccgtcg ccggcgttga tcacgtgtgc
     4321 gtccacccac tgggccaggc ggtgcggcgc gcccgaggcc ttgtgacgga tcacgatcgc
     4381 gtccacgccc atcgccatga tggtttgcgc ggtgtccttg agcgactcgc ctttctcgac
     4441 ggccgagccg gagaccgcga agttgaccac gtcggcgctc agccgcttgg cggcgagctc
     4501 gaacgaggta cgcgtgcgcg tcgaggactc gtagaacagg ttcacgaccg tgcggttgcg
     4561 aagcgccgga tacttgcgga tcgcaccccc gtcgggctgg aggttcgcgg cggtctcgag
     4621 gatgcgctcg atgccgtcgc ggtccaggtc ggtgatgtcg agcagatgct tgatcatgat
     4681 tcctcccgct cggccaccag gacggcgtcc tcgccgtcgg cctcggcgac acgcaccacg
     4741 atctcctcgc cgtgcgcggt cgggatgttc ttgccgacgt agtcggcccg gatcggaagc
     4801 tcgcgatggc cgcggtccac caagaccgcg agctgaacgg ccttgggccg gcccacatcc
     4861 atgagcgcgt ccagcgctgc tcggacgctg cggccggtgt agagcacgtc gtcgaccagc
     4921 acgacgatct tgtcgtcgat cggcgggatg tggtcggtct tgagctcggc gggcgcgcgc
     4981 aggccgatgt catcgcggta caggccgatg tcgagggacc cggtcgggac cttgacgccc
     5041 tcgatgtcgg cgatccgctc ggcgaggcgc tgggcgatga tctcgccccg ggacagcagg
     5101 ccgaccagca ccagatgctc ggcgcccttg tttcgctcga ggatctcgtg ggcgatgcgc
     5161 gccagcgccc gggacaggtc cccggcgtcc atcggcgcgg ccttcggctt gccgctcagc
     5221 gggctcgcct cccccaccgc ggcgacccgg aggggggaag ggagatggaa aaaagaaaag
     5281 cccgcgccga tgcgcaggcc gacccgtgct gcttcaaggc gttctctctc ccttccgggc
     5341 ctcacaggac ccgcccttaa agggtgtgtg gggtcgagta tagcggcggc gcggccgggc
     5401 ttcaatgcga tggacgtgga accccggctg cgccgcgccg tctcaatgct ttcgtcgccg
     5461 aagcgtggat gcgagggcgg ccgctcccga caccgccaca agcccggccg ggatcgcgga
     5521 cccgacaccc gtggcgggca gcgcggtccg tgcgccgcgc acgacggcgg gtttcggagc
     5581 aggctttgga gcgggctcgg ccgaggcttg cttgaatgcc ttggcacgcg tgaccttcgg
     5641 tgtgcgcacc ccgagcacgt cgaggccgcg gaccgcgtcg ctcacgtaga cgatgacctc
     5701 gccgggatcg tcggggttgc gtccggcggc ccaagcctgg atcgccatcg agccggcggg
     5761 aacggtcgag gcaacctctt cgaagccgat cgtcgccggc gaggtggtca ggtcgagcgt
     5821 gtagcggaag acgctggttc cggcgtgata ccaggcgaag gccagcaggt tttcgtcggc
     5881 aaactcttgc ccgtaatgcg aggtgcacga cacggactgt ccgaacggcg ggggcgggct
     5941 tgaggcggtg cggaacatgc cggggctacc gtcgctgcgc ttcatcagct tcggcttggt
     6001 ctcgtcgcgg atgtcgtaga accagatccc gccgccggga cagccctgga cgttggccag
     6061 gccgccgccg cgttcgtcgg tgatcagcat ggtggttccg tccccgatga cctcggcttg
     6121 gtgggcaatc tcgatgtcct gctcggcggg cttggtgttg tcgtgctgga tccaggacac
     6181 cagccggatg tccatcggac gggccttggc cgcgatgaac tgttccggcg tccaggccat
     6241 gcagtcggtg attttcacgc cgggagcgcg cacggcgtcg atgaccggac agccgttgtt
     6301 gtcggtgagc ttggtgccgg tcagccggcc gtccgctccg accatcccgg agatgtccag
     6361 gatcagcgtg gtgttcacgg cggcgcagta gacgcggtcg ccgcggaacc gaacgtcgtg
     6421 gcagccg
//
LOCUS       DATLFO010000319        11653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_28777, whole genome shotgun sequence.
ACCESSION   DATLFO010000319 DATLFO010000000
VERSION     DATLFO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28777"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VM841_12925"
     CDS             complement(<1..1289)
                     /locus_tag="VM841_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HVL91128.1"
                     /translation="MRRLALVLHSHVPYVRRNGVWPAGEDMFHQVASESYLPLLGVLE
                     RLADRGMHSALTIGITPVLAHQMQDAHMLRELEAYLARIELRALRQVANYRLIHEREV
                     KDLAAVYARAGRDAAARLARCGDGMARAFAAFARDGLIEILGGPATHPLLPLLSSEIT
                     RRLQMTVGAAEHERVFGGRASGAWIPECAVAPGVETCLAAANVAYSVVDPAAAGGAAE
                     PVRAADTEVLLVPRDRVLTDLVWAMDGYPSGEWYRDFGHHDTVGGFKNWRVTAPGATL
                     GDKQPYEPAAARRAATEDARHFVAAVERRFDETGRSDLVVAFDTELFGHWWFEGPVWL
                     EEVLSALAVHPSVVPATLGEIAASHAGAARAAPAASSWGAGSDFRNWQSGRTDTLFER
                     ARDAEARAIPLLGRIGPVRDQMVRELLLLQSSDWPYQ"
     gene            complement(1297..2046)
                     /locus_tag="VM841_12930"
     CDS             complement(1297..2046)
                     /locus_tag="VM841_12930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL91129.1"
                     /translation="MPTEPEYVFDDAIPLTGERTAPGIPEENYWFQRHVIAYEWAAAR
                     AAGKRVLDAGCGEGYGTQILAAKAAEVVGVDLEAGVVERAAARYPSARFEPADLEALP
                     YPDASFDAVVSLQVIEHMRSPIEFCSEVARVLRPGGEFLCATPNRLTFSPEGIRNPFH
                     TVEFSPDELRGTLGRHFTVVEMLGTFHAGRIRAGEIATRQSFPERLIETPVPSWSPRL
                     RAAVARITTRDFRIRGENLDRSLDLIAVGRR"
     gene            2158..3591
                     /locus_tag="VM841_12935"
     CDS             2158..3591
                     /locus_tag="VM841_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91130.1"
                     /translation="MAKPWDQRSAAEQRRVQNGLVNATLVQMALAHVPFVRAKLQKAG
                     VDARLIRRVEDLKKVPLSMRRDVVDPVRNPEGPGAFVLKGTAEGVKRFSDRSVLWRVA
                     LSRLFGGEEVQALAIEAASRSVHLHLSPGPGGPIPVSYTRDDLDLLARAGSRLAAVAG
                     VDREDRLLNLVPFGSTLDFWGVFYMAHGVGMTALHTRREGADLAQALATGFASEPATA
                     VAVPADEAVSFPGAAAAAGVPLDSLGLMLAVGRSLTRSERERLGEEFAKAGAPNVRIA
                     AAYGPAEGRVLWGECAVPAGTVETFGFHTYPDLDIVEIAQPDTGDVLPDETPGEIVVT
                     PLGFRGGGAPRWRTGDLALGGVTQRPCPNCGRQVPRVGPSVRRNAWMRRIVLGGRRVR
                     FDFRDAASALAGAKDWQIELKAADKEHELFVYVVGSESDVGPVISLYEAMERWGVPPT
                     QIVLASEQEIAEKKKAIESSWPRFAEI"
     gene            3691..5868
                     /locus_tag="VM841_12940"
     CDS             3691..5868
                     /locus_tag="VM841_12940"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVL91131.1"
                     /translation="MRSPSPKRFALRAVTLALSGLLSSLAFVAAPVSAEPEHAATQAK
                     DVTIQSAATRFMAGTFALPIRANMIGLTYRGSGAEIEMRIHRPGQGWGPWEEIDSSDI
                     APDRWTGEGNDRSATEPVWAGTADSVMVRMKGETGNVRDVRLHAINTLGDAEPKTWWK
                     RLFAFLGSAPASLAPRTQAAEAVPAVPEIVSRAQWGADERWRRCCPRYAPTVEVMMIH
                     HTVNPNNYSWDEAPAMLRAIYRYHRFNLDYDDIAYNFIVDRFGRIFEGRFGGVTKPVV
                     GAHTEGMNTKTSGIALLGTFSNAYPSGPMMVSLMRFLAWKLDVHHIPGWGHVPMTSGG
                     SKKFARGQTVNMNRISGHRDAQVTDCPGTRMYYQLPRIRETIMPWGAPKFYMNSTPRE
                     LRADGDDKDEGPIDVKVWNSSELNWKIDFADAAGVAKASFSGKGTFAQATWDGKDQVS
                     GALARSGVGTITVDAWDDNNKHATKAEIPMRIVNKHPNGTVLRSGTTRVFIDDAGASR
                     SIPSDTVYASWFGPNEIVSTGPAEIPRYTAGLPLGFRDGTLAKTPDGAYHFINNGQRR
                     AFASPAVFQTLGFRDEAAIQVSHADVNALPSGPVIEDATRHPAGSVVEDADKVAWVVR
                     DGTKHRIPNSAVGRSWYRDAEVVPALPGDTALPEGEQIQFRSGTLLRTPSQHVWIVSN
                     GTRLVFFDEELFAFMGYSTKAIRTVTWGEIGVLPYGGTVSGNV"
     gene            5912..7057
                     /locus_tag="VM841_12945"
     CDS             5912..7057
                     /locus_tag="VM841_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HVL91132.1"
                     /translation="MQTPTSLYLDYAATTPLLDEAREAMMPWLAGGFANASGLYAGAR
                     EARKAVDEARETLAAAVGARPEEVVFTSGGTEADNLAVKGAAWHGRDQGRDGIVISSF
                     EHHAVLDPADWLTRFGFRVTRVPVSGDGVVDLDALRAAVDDKTAIVSVMWANNEAGTI
                     QPVAEAAAIAHERGAVFHSDAVQALPWIPVGIADADMISLAAHKCHGPKGVGALVLRR
                     GTRLQPLLHGGGQERGLRSSTYNTPGIAGFAAGVAAAMRDRDEAAVRVAALRDRLQDA
                     LTGRIPGAHVNAAGAPRLPNHLSVSFERIESETLLLLLDASGVAASAGSACQSGASEP
                     SYVLRAMGVPDALASGVLRLTLGRPTTQDEVDRAADAIVAAVERLRA"
     gene            7054..8121
                     /gene="mnmA"
                     /locus_tag="VM841_12950"
     CDS             7054..8121
                     /gene="mnmA"
                     /locus_tag="VM841_12950"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVL91133.1"
                     /translation="MSKVLVAMSGGVDSSVAAALLVEQGHEVTGVHLKLSDYAAPQQE
                     GRVHGCCSVEAADDARRVAQVLDMPFYVWNLVEPFRAAVVDDFVGEYAAGRTPNPCVR
                     CNERVKFGAVLERGLALGFDYVATGHYAVARRDGGRARLYRSADAAKDQSYVLSSIGR
                     DALERSLFPVGPMTKDRTREIARDLSLRTADKPESFDICFVPGGDAGAFVDAAIGTRP
                     GPIVTGDGAVVGEHGGTHRFTVGQRRGLGLATHERQFVVDIDAASNTIVVGSGELLSR
                     RTIEAERIRWFDAPPASGAAVAVQIRAHGRVIPATLAAAGDEAAEVRLDELERGVAAG
                     QTIAFYDGDEVLGGGIISSAR"
     gene            8187..8375
                     /locus_tag="VM841_12955"
     CDS             8187..8375
                     /locus_tag="VM841_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91134.1"
                     /translation="MDNVRLPDLETIRRFSIAVRTEHAPAEEVLEALRVDLERIERAI
                     GTRRPARSSSSSAARRRR"
     gene            8398..10437
                     /gene="ligA"
                     /locus_tag="VM841_12960"
     CDS             8398..10437
                     /gene="ligA"
                     /locus_tag="VM841_12960"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVL91135.1"
                     /translation="MRKDEARKRIEALRTEIERHDYLYHVLDAPEISDAAYDELFHEL
                     KALEEQFPELVTLDSPTQRVGGGFKGSQFEQVTHGRRMYSLDNAFDDDELAAWAERVA
                     KGVGDVRYVCELKIDGLAVALTYQDGRFVRGATRGDGIVGEDVTANLRTIRGIPTKLR
                     IAKPPALIEVRGEVYMPTKAFAEMNDSLRDAGKEGFANPRNAAAGALRQKDPAVTASR
                     PLTYWIHGSGAIEGVRFAAHREFLDWCKEAGMRVAPTTKIVEDLDGVRAFIAHWAEHR
                     HDLEHEIDGVVVKVDSIAQQDELGYTAKAPRWAIAYKYPPEERETILKGIRVHVGRTG
                     AVTPYAVLEPVFVGGVTITTATLHNQDEVARKDVRPGDTVIVRRAGDVIPEVAGPVLS
                     KRPKNTRRWKMPAACPYCGSEILREEGEAVAHCTGIDCPSQRVERIAHFAGRGALDID
                     GLGYRTIIELTDTGLVSDAGDLYSLTDEQILSLEGFASEKDKTTGETVPGKRARNLRA
                     AIEASKDRPLARLLTGLGIRHVGGTVAQTLARHFSSLDALEAASEEEITAVEGIGPVI
                     AHSVHEFFRQPRNVKVLEKLRAAGLRIADESPKAAADGPLSGKTVVLTGGLESMTREQ
                     ATEAIEAAGGKVASSVSKKTSYVVAGASPGSKYDKAVRLGIEIIDETGLSELLGH"
     gene            10530..>11653
                     /locus_tag="VM841_12965"
     CDS             10530..>11653
                     /locus_tag="VM841_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91136.1"
                     /translation="MRTRISRFVSVVAAVLAVTAIAAFSPVNAGPKSCPPGYTPIEDF
                     GVQAQDERGVLCISGKHPESLLEMEQRDSQRESVATAPLSPGETVGLYRASLEERRSV
                     LAQGANGVPGAAGTWVPYAKGPLRSDDPRFQNTNGFGFAELGGRIDSLDYDPVNKRLF
                     ASIGTGGVWMSENLGEKWVSIGDRLPSQIVGAVAWSPAKGGTLVVVSGEPIVGGYTRT
                     GIGAFFSRDLGKTWHQSKGVPDGALGFQVAVDPTKPEVVYAATGKGLYRSADAGATFV
                     NVKLPTGPCAGKTDNDKCLLANQVTDVIVQSPDKFGHKGGRVLAAVGYRSGPRPFPQD
                     KSVIESPGNGLYRSDTGLPGSFAKLDAPGFTPQHRIGRTEL"
BASE COUNT         1801 a         4109 c         4101 g         1642 t
ORIGIN      
        1 atctggtacg gccagtccga ggactgcagc aggagaagct cacggaccat ctggtcgcgc
       61 accggcccga ttcggccgag cagcgggatc gcacgggcct cggcgtcccg ggcgcgctcg
      121 aacagcgtgt cggtccggcc ggactgccag ttccggaagt cggagcccgc gccccacgac
      181 gacgccgcgg gcgccgcacg cgcggcgccc gcgtggctcg cggcgatctc accgagcgtc
      241 gccggcacga ccgacggatg gaccgcgagc gccgatagca cctcctcgag ccagaccggc
      301 ccttcgaacc accaatggcc gaacagctcg gtgtcgaacg caacgaccag atcgctgcgt
      361 ccggtctcgt cgaagcgccg ctcgacggcc gcgacgaagt ggcgcgcgtc ctccgtcgcg
      421 gcccgccgcg cggccgcggg ctcgtagggc tgcttgtcgc cgagcgtggc tccgggcgcc
      481 gtcacccgcc agttcttgaa tccgccgacg gtgtcgtggt ggccgaagtc gcggtaccac
      541 tcgccggacg gatagccgtc catcgcccag accagatcgg tcaacacacg gtcgcgcggc
      601 accagcagca cctcggtgtc tgccgcgcgc accggctcgg ccgcgccgcc ggccgccgcg
      661 ggatccacca ccgaataggc gacgttcgcg gcggcgaggc aggtctcaac acccggcgcg
      721 acggcgcact cggggatcca cgcccccgac gcccgtccgc cgaacacgcg ctcgtgctcg
      781 gcggccccga ccgtcatctg gagccgccgc gtgatctcgg aggaaagcag cggcagcaac
      841 ggatgcgtcg ccggcccgcc gaggatctcg atcagcccgt cgcgtgcgaa ggccgcgaac
      901 gcgcgcgcca tgccgtcacc gcagcgcgca aggcgtgccg ccgcgtcgcg gcccgcgcgg
      961 gcgtagacgg cggcgaggtc cttgacctcc cgctcgtgga tcagccggta gttggcgacc
     1021 tgccggagcg cccgaagctc gatccgggcg agataggcct cgagctcgcg cagcatgtgc
     1081 gcgtcctgca tctgatgcgc cagcaccggt gtgatgccga tcgtcagcgc gctgtgcatc
     1141 ccccggtcgg cgagccgttc cagcacgccc agcagcggaa ggtaggactc gctcgcgacc
     1201 tgatggaaca tgtcctcgcc cgcgggccac acgccgttgc gccgcacgta gggtacgtgc
     1261 gagtgaagga cgagggcgag ccggcgcacg gtcgcctcag cggcggccga cggcgatgag
     1321 gtcgaggctg cggtccaggt tctcgccgcg gatgcggaag tcccgcgtgg tgatgcgcgc
     1381 gacggcggca cggagccgcg gcgaccagct cggcaccggc gtctcgatca gccgctccgg
     1441 gaaggactgg cgcgtggcga tctcgccggc gcggatccgc ccggcgtgga aggtgccgag
     1501 catctcgacg accgtgaagt gacggccgag cgtgccgcga agctcgtccg gcgagaactc
     1561 aacggtgtgg aacgggttgc ggatgccctc gggcgagaag gtgagccggt tgggcgtggc
     1621 gcacaggaac tcgccgcccg gacgcagcac gcgcgcgacc tcgctgcaga actcgatggg
     1681 cgagcgcatg tgctcgatca cctgcagcga cacgaccgcg tcgaaggatg cgtcgggata
     1741 ggggagcgcc tccaggtcgg cgggctcgaa ccgagccgac ggatatcggg cggcggcgcg
     1801 ctcgacgacg ccggcctcca ggtccacccc gaccacctcg gcggccttcg cggccaggat
     1861 ctgcgttccg tatccctcgc cacagccggc gtccaggacg cgcttgccgg cagcccgcgc
     1921 ggccgcccat tcgtaggcga tgacgtggcg ctggaaccaa tagttctcct cggggatgcc
     1981 gggcgcggtg cgctcaccgg tcagggggat cgcgtcgtcg aagacgtatt cgggctcggt
     2041 cggcatcggg gcgcatcata cccgcccgga ccgccctgac cggcggcgcg aggcgcgtcc
     2101 ggccccgctt cctgcctgct gcgcgaagcc aaccgtccac ccgtacaatg tcccgccatg
     2161 gccaagcctt gggaccaacg cagcgccgcc gagcagcggc gtgttcagaa cggcctcgtc
     2221 aacgcgacgc tcgttcagat ggcgctcgcg cacgtgccgt tcgttcgcgc gaagcttcag
     2281 aaggccgggg tggacgcgcg gctgatccgc cgcgtggagg acctcaagaa ggtgcccctg
     2341 tcgatgcgcc gcgatgtggt cgatccggtc cgcaaccccg aagggcccgg cgcgttcgtg
     2401 ctcaaaggca ccgccgaagg ggtgaagcgc ttcagcgacc ggagcgtgct gtggcgtgtg
     2461 gcgctgtcac ggctgttcgg tggggaggaa gtccaggcgc tggccatcga ggcggcgagc
     2521 cgcagcgttc acctgcacct gtcgccgggg cccggcgggc cgatcccggt ctcctacacc
     2581 cgcgacgacc tcgatctgct ggcgcgggcc ggctcgcgcc tggccgccgt ggccggcgtg
     2641 gaccgcgagg accggctgct gaacctggtt ccgttcgggt cgacgctcga cttctggggc
     2701 gtgttctaca tggcccacgg cgtcggcatg accgcgctgc acacgcgccg cgaaggcgcc
     2761 gacctcgcgc aggcgctcgc gaccggcttc gcgtccgagc cggcaaccgc ggtcgcggtg
     2821 cccgccgacg aagccgtgtc gttccccggc gcggccgccg cggccggcgt gccgctcgac
     2881 tcgctcggct tgatgcttgc ggtcggccgc tcgctcacgc gctcggagcg cgagcgactc
     2941 ggcgaggagt tcgccaaggc gggcgcgccg aacgttcgca tcgcggcggc ctacggtccg
     3001 gccgaagggc gggtgctgtg gggtgagtgc gcggtcccgg ccgggaccgt cgagaccttc
     3061 ggcttccata cctatccgga tctggacatt gtcgagatcg cccagcccga caccggcgac
     3121 gtgcttcccg acgagacgcc cggcgagatc gtggtcacgc cgctcgggtt tcgcgggggc
     3181 ggcgcgccgc gctggcgcac cggcgatctc gcgctcggcg gcgtcacgca gcggccctgc
     3241 ccgaactgcg gccggcaggt gccccgggtc ggcccgagcg tccggcgcaa tgcgtggatg
     3301 cgacgaatcg tgctcggcgg gcggcgcgtg cgcttcgact tccgcgatgc ggcgagcgcg
     3361 ctcgcgggcg cgaaggactg gcagatcgag ctcaaggccg ccgacaagga gcacgagctg
     3421 ttcgtgtacg tggtgggcag cgagtccgac gtcggtccag tcatctccct gtacgaggcg
     3481 atggagcgat ggggcgttcc tccgacacag atcgtgcttg cgtccgagca agaaatagcc
     3541 gagaagaaga aggcgatcga atcttcttgg cctcgattcg ccgaaatcta gggacagaag
     3601 ccgaaatctg ggcatgcacc ctgcttgacc ccgcaacgcc cggctcgtag tctgcccgag
     3661 gttccgatcg agccgccaac caccatgcag atgagatcac cttccccgaa gcggttcgca
     3721 ttgcgcgccg ttaccctcgc tctgtccggg ctgctctctt cgcttgcttt cgtcgccgcg
     3781 ccggtttcgg ccgagcccga gcacgcagca acgcaagcca aggacgtgac gattcagtcc
     3841 gccgccactc gcttcatggc cggcacgttc gcgctgccga tccgcgcgaa catgatcggg
     3901 ctgacctacc gcggcagcgg cgccgagatc gagatgcgca ttcaccgtcc cggacaggga
     3961 tggggtccgt gggaggagat cgactccagc gacatcgcgc ccgaccgctg gaccggcgaa
     4021 ggaaacgacc gcagcgcgac cgagcccgtc tgggccggca ccgccgattc ggtgatggtc
     4081 cgcatgaagg gcgagaccgg caacgtgcgc gacgtgcgct tgcacgcgat caacacgctc
     4141 ggcgacgcgg agccgaagac atggtggaag cgcttgttcg ccttcctggg ctccgcgccc
     4201 gcgtcgctcg ctccgcgcac gcaagcggcc gaagcggttc cggccgtgcc cgagatcgtc
     4261 agccgcgcgc agtggggcgc cgacgagcgg tggcgccgct gttgcccgcg ctacgcgccg
     4321 accgtcgagg tgatgatgat tcaccacacg gtcaacccga acaactactc ctgggatgag
     4381 gcgccggcga tgctccgggc gatctatcgc taccaccgct tcaacctgga ctacgacgat
     4441 atcgcctaca acttcatcgt cgatcgcttc ggccggatct tcgaaggacg gttcggcggc
     4501 gtgaccaaac cggtcgtcgg cgcgcacacc gaaggcatga acaccaagac ctcgggcatc
     4561 gcgctgctcg gcacgttctc gaacgcatac ccgagcgggc cgatgatggt gtcgctgatg
     4621 cgcttcctgg cgtggaagct cgacgtgcat cacatccccg ggtggggtca tgtgccgatg
     4681 acctcgggcg ggagcaagaa gttcgcgcgc ggccaaaccg tgaacatgaa ccgcatctcg
     4741 ggccatcgcg acgcgcaagt aaccgactgc ccggggacga ggatgtacta ccagcttccc
     4801 cgcatccgtg agacgatcat gccgtggggc gcgccgaagt tctacatgaa ctccacgccg
     4861 cgcgagctgc gcgccgacgg cgacgacaag gacgaagggc ccatcgacgt caaggtctgg
     4921 aattcgtccg agctgaactg gaagatcgac ttcgcagacg cggccggcgt ggcgaaggcg
     4981 agcttcagcg gcaagggcac gtttgcgcag gcgacctggg acggcaagga ccaggtctct
     5041 ggtgcgctgg cgcgaagcgg ggtcggcacg atcactgtag acgcgtggga cgacaacaac
     5101 aagcacgcga ccaaggccga gatcccgatg cgcatcgtga acaagcatcc caacggcacc
     5161 gtgcttcggt cggggacgac ccgcgtcttc atcgacgacg cgggcgcgtc ccggtcgatc
     5221 ccgtcggaca cggtgtatgc gtcctggttc gggccgaacg agatcgtaag caccggcccg
     5281 gccgagatcc ctcggtacac ggccgggctt ccgctcggat tccgcgacgg cacgctcgcc
     5341 aagacgcccg acggcgcgta ccacttcatc aacaacggac agcggcgggc gttcgcgtcc
     5401 ccggccgtgt tccagacgct cggcttccgc gacgaggccg cgatccaggt ctcgcacgcc
     5461 gacgtcaacg cgctgccgtc ggggccggtc atcgaggacg cgacccggca tcccgcgggc
     5521 agcgtggtcg aggacgccga caaggtcgca tgggtcgtgc gcgacggcac caagcaccgg
     5581 atccccaaca gcgcggtcgg gcgctcttgg taccgcgacg ccgaggtcgt gcccgccctg
     5641 ccgggcgaca ccgctctgcc cgagggcgag cagattcagt ttcgttccgg cacgctgctg
     5701 cgcacgccga gccagcacgt gtggatcgtg tccaacggca cgcgtctggt gttcttcgac
     5761 gaggagctgt tcgcgttcat gggttactcg acgaaggcga tccgcacggt gacctggggc
     5821 gagatcggcg tgcttcccta cggcggaacc gtcagcggaa acgtctgaaa cgccgggaac
     5881 cgcacgcggt gcgggtgcta cccttgatcc gatgcagacc ccgacctccc tctacctcga
     5941 ctatgcggcg accacgccgc tgctggacga ggcccgcgag gcgatgatgc cgtggctcgc
     6001 cggcggcttc gccaacgcga gcggcctgta cgcgggagct cgcgaggcgc gcaaggcggt
     6061 cgacgaggcg cgtgagacgc tcgcggcggc cgtcggtgcg cggcccgagg aggtcgtgtt
     6121 cacctccggc gggaccgaag ccgacaacct cgccgtcaag ggcgcggcat ggcacggccg
     6181 cgatcaaggc cgcgacggca ttgtgatctc gtccttcgag caccacgccg tgctcgaccc
     6241 ggcggattgg ctgactcgct tcggctttcg cgtcacgcgt gttccggtgt ccggggacgg
     6301 cgtcgtcgat ctcgacgcgc tgcgtgccgc ggtggacgac aagaccgcga tcgtgtcggt
     6361 gatgtgggcc aacaacgagg ccggcacgat ccagcccgtc gccgaggccg cggcgatcgc
     6421 gcacgaacgc ggcgcggtct tccactccga cgcggtgcag gcgctgccgt ggatcccggt
     6481 cggcatcgcc gatgccgaca tgatctcgct tgccgcgcac aagtgccacg ggcccaaggg
     6541 cgtcggcgcg ctggtgctgc gtcgcggcac ccggctgcag ccgctgctgc acggcggcgg
     6601 ccaggagcgc ggccttcgat cctcgaccta caacacgccg ggcatcgccg gcttcgccgc
     6661 gggggtcgcc gccgcgatgc gcgatcgcga cgaggccgcc gtgcgcgtcg ccgcgctgcg
     6721 cgaccgtctg caggacgcac tgacgggccg catcccgggt gcgcacgtca acgcggccgg
     6781 cgcgccgcgc ctgcccaacc atctgtccgt cagcttcgag cgcatcgaaa gcgagacgct
     6841 gctgctgctg ctcgacgcct cgggggtggc ggcgtcggcg ggaagcgcgt gccagtcggg
     6901 cgcgtccgag ccgtcgtacg tgctccgcgc gatgggggtg cccgacgcgc tcgcgagcgg
     6961 cgtgctccgc ctgacactgg ggcggccgac gacgcaggat gaggtcgacc gggccgcgga
     7021 cgcgatcgtg gcggccgtgg agcggctgcg cgcatgagca aggtcctggt tgcgatgagc
     7081 ggcggcgtcg attcgtcggt cgccgccgcg ctgctcgtcg agcagggcca cgaggtcacc
     7141 ggcgtgcatc tgaagctgtc ggattacgcc gcgccccagc aggaaggccg cgtgcacggc
     7201 tgctgcagcg tcgaggcggc cgacgacgcg cgacgcgtcg cacaggtgct cgacatgccg
     7261 ttctacgtgt ggaacctggt ggagccgttc cgcgccgccg tcgtcgacga cttcgtcggc
     7321 gagtacgccg ccggccgcac gcccaacccg tgcgtgcgct gcaacgagcg tgtgaagttc
     7381 ggggcggtgc tcgaacgcgg tctcgcgttg ggcttcgact acgtcgccac gggccactac
     7441 gcggtcgcgc ggcgcgacgg cgggcgcgcg cggctgtatc gcagcgccga cgcggccaag
     7501 gatcagtcgt acgtgctgtc ctcgatcgga cgcgacgcgc tcgagcgctc gctgttcccg
     7561 gtcggcccga tgaccaagga ccgcacccgc gagatcgccc gcgacctgtc gctgcgcact
     7621 gccgacaagc ccgaatcgtt cgacatctgc ttcgtcccgg gcggcgacgc gggcgcattc
     7681 gtcgacgccg cgatcggcac gcggcccggc ccgatcgtga ccggcgacgg cgcggttgtg
     7741 ggtgagcacg gcgggacgca ccggttcacc gtgggtcagc gccgcggcct cggcctggcg
     7801 acccacgagc ggcagttcgt ggtggacatc gacgcggcga gcaacaccat cgtcgtcggc
     7861 tccggcgagc tgctgtcgcg ccgcacgatc gaagcggagc gcatccgctg gttcgacgcg
     7921 ccgccggctt cgggcgccgc ggtcgcggtg cagatccgcg cgcacggccg cgtgatcccg
     7981 gcgacgctcg cggccgccgg ggacgaggcc gccgaggtcc ggctcgacga gctcgagcgc
     8041 ggtgtcgcgg cgggccagac gattgccttc tacgatgggg acgaggtcct cggcggcggc
     8101 atcatctcct cggctcgcta gcatccgagc gcgccggcgt gcgctcgtat aatcacgcgg
     8161 cagaccccga cgcagtggag gacatcgtgg acaacgttcg cctgcccgac ctcgagacga
     8221 tccggcgctt ctcgatcgcc gtccgcaccg agcacgcacc ggccgaggag gtcctcgaag
     8281 cgctgcgcgt cgacctggag cggatcgagc gagccatcgg gacccgccgc ccggcgcgct
     8341 catcgtcgtc gtcggcggct cgccggcgcc gctagcgccc gcgagccgca ggccgcgatg
     8401 cgaaaggacg aggcacgaaa gcgcatcgag gcgctccgaa ccgagatcga gcgtcacgac
     8461 tacctctacc acgtcctcga cgcccccgag atcagcgacg cggcctacga cgagctgttc
     8521 cacgagctga aggcgctcga ggaacagttc ccggagctcg tcacgctcga ctcgccgacc
     8581 cagcgcgtcg gcggcggctt caagggatcg cagttcgagc aggtcaccca cggccggcgg
     8641 atgtattcgc tggacaacgc cttcgacgac gacgagctcg ccgcctgggc cgagcgcgtc
     8701 gccaaaggcg tcggcgacgt ccgctacgtg tgcgagctca agatcgacgg gctcgcggtc
     8761 gcgctcacct atcaggacgg ccggttcgtg cgtggcgcga cccgcggcga cggcatcgtc
     8821 ggcgaagacg tcaccgcgaa tttgcgcacc atccgcggca tccccaccaa gcttcgcatc
     8881 gcgaagccgc ccgcgctgat cgaggtgcgc ggcgaggtct acatgccgac gaaagccttc
     8941 gccgagatga acgactcgct ccgggatgcc ggcaaggagg ggttcgcgaa tccccgcaac
     9001 gccgccgccg gcgcgctgcg ccagaaggat cccgccgtca ccgcctcgcg gccgctgacc
     9061 tactggatcc acggtagcgg cgcgatcgag ggcgtgcgct tcgcggcgca ccgcgagttc
     9121 ctggactggt gcaaggaggc gggcatgcgc gtcgcgccca cgacgaagat cgtggaggac
     9181 ctcgacggcg tgcgcgcgtt catcgcgcac tgggccgagc accgccacga cctcgagcac
     9241 gagatcgacg gggtcgtggt caaggtggac tcgatcgcgc agcaggacga gctcggctac
     9301 accgccaagg caccgcgctg ggcgatcgcc tacaaatatc cgcccgagga gcgtgaaacc
     9361 atcctgaagg gtatccgcgt tcacgtcggg cgcaccggcg ccgtgacacc gtacgcggtg
     9421 ctcgagccgg tcttcgtcgg cggcgtcacg atcaccaccg cgaccctgca caaccaggac
     9481 gaggtcgcgc gcaaggacgt gcggcccggc gacaccgtga tcgtgcggcg tgcgggcgac
     9541 gtcatccccg aggtggccgg cccggtcctg tcgaagcgtc ccaagaacac ccgccgctgg
     9601 aagatgcccg cggcctgtcc ctattgcggc agcgagatcc ttcgcgagga aggcgaggcc
     9661 gtcgcgcact gcaccggcat cgactgcccg tcgcagcggg tcgagcggat cgcgcacttc
     9721 gccggccgcg gcgcgctcga catcgacggg ctgggctacc gtacgatcat cgagctgacc
     9781 gacacaggtc tggtgtcgga cgcgggggac ctgtactcgc tgaccgacga gcagatcctg
     9841 tcgctggagg gcttcgcgtc cgagaaggac aagacgaccg gcgagaccgt tcccggcaag
     9901 cgcgcccgca acctgcgcgc cgccatcgaa gcgtcgaagg accggccgct cgcgcggctg
     9961 ctcaccggcc tcggcatccg ccacgtgggc ggcaccgtcg cccagacgct ggcccgccac
    10021 ttctcgtccc tcgacgcgct cgaggcggcg agcgaagagg agatcaccgc cgtcgaaggc
    10081 atcggccccg tgatcgccca ctccgttcac gagttcttcc gccagccgcg caacgtcaaa
    10141 gttctcgaaa aattgcgcgc cgccggtctc cgaattgcgg acgaaagccc gaaggcggcc
    10201 gcggacggcc cgctgtcggg caagacggtc gtgctcaccg gtggcctgga gtccatgacc
    10261 cgcgagcagg cgaccgaggc gatcgaggcc gcgggcggca aggtcgcgag cagcgtctcc
    10321 aagaagacct cttacgtcgt ggccggagcc agtccgggct cgaagtacga caaggccgtc
    10381 cggttgggga tcgagatcat cgacgaaaca ggcctttcgg aactgctcgg ccactgaccg
    10441 tcttgcccgg tcacgcccgg tccgcgatac tttcatggaa cgggacaaac cagccgtgtc
    10501 acggcgatct cgcttcggag ggctttcgca tgcgcacccg gatctctcgc ttcgtatcgg
    10561 tcgtggcggc cgtgctcgcg gtcaccgcca tcgccgcctt ctcaccggtc aacgccggac
    10621 ccaagtcctg tccgccgggc tataccccga tcgaggactt cggcgtccag gcgcaggacg
    10681 agcggggcgt actgtgcatt tcgggcaagc atcccgagtc gctcctggag atggagcagc
    10741 gggacagtca gcgcgagtcg gtcgcgaccg cgccgctgtc gccgggcgag acggtcggtc
    10801 tgtaccgcgc atccctcgag gaacgccgct ccgtgctcgc gcagggcgcg aacggcgtgc
    10861 ccggcgcggc cggcacctgg gtgccctacg ccaagggtcc gctgcgctcg gacgatcccc
    10921 ggttccagaa cacgaacggc ttcggtttcg cggagctcgg cggccgcatc gacagcttgg
    10981 attacgaccc ggtcaacaag cgcctgttcg cgtcgatcgg gaccggcggc gtgtggatga
    11041 gcgagaacct cggcgagaag tgggtctcca tcggcgaccg gcttccctcg cagatcgtgg
    11101 gcgcggtcgc ctggtctccc gccaagggag gcaccctggt ggtcgtgagc ggcgagccga
    11161 tcgtaggcgg ctatacccgg acagggatcg gcgcgttctt ctcgcgcgac ctgggcaaga
    11221 cgtggcatca gtcgaagggc gtgcccgacg gcgcactcgg cttccaggtc gcggtggacc
    11281 cgaccaagcc cgaggtcgtc tacgccgcca ccggcaaagg cctgtatcgc tccgcggacg
    11341 cgggcgcgac cttcgtgaac gtgaagcttc ccacagggcc gtgcgccggc aagacggaca
    11401 acgacaagtg ccttctggcg aaccaggtca ccgacgtgat cgtgcagtcg cccgacaagt
    11461 tcggccacaa gggcggccgc gtgctcgcgg cggtcggcta ccgcagcgga ccacgcccct
    11521 tcccgcagga caagagcgtg atcgaatcgc ccggcaacgg cctgtaccgg tccgacaccg
    11581 gcctgccggg ctcgttcgcg aagctggacg cgcccggctt cacgccgcag caccgcatcg
    11641 gccgcaccga gct
//
LOCUS       DATLFO010000320         4870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_17422, whole genome shotgun sequence.
ACCESSION   DATLFO010000320 DATLFO010000000
VERSION     DATLFO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17422"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VM841_12970"
     CDS             <1..487
                     /locus_tag="VM841_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HVL91137.1"
                     /translation="ISPGKANMGIIPGEITGPGPIGLLSRSGTLTYQVMYEMFTRGVG
                     QSTCVGIGGDPIVGSNFIDILGRFQADDETQAIVLIGEIGGDEEEQAARFIADNVTKP
                     VVAYIAGHTAPPGKRMGHAGAIISGSKGTAQAKKEAFESLGIKVGINPTEVAELAVKS
                     L"
     gene            complement(680..2197)
                     /locus_tag="VM841_12975"
     CDS             complement(680..2197)
                     /locus_tag="VM841_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91138.1"
                     /translation="MTPPETVADAHHRYRGAVERAAARILRDREDASDIASETFLTML
                     ERGPRDRQAALAWLLTTARNRALNLVRDRQRAARRHLMLVEDFEEPAVSDERLASLVA
                     SAMSRLSDRDQAAVLLRFVDDCSPQEIAATLGTSVAASRVVVHRAVKRLRVETVRVLA
                     EHHGVDGACASRLAKAASDGIPAPHAGCSPCSSVADEITALAAHSLLPVGAAPVLHSI
                     AGRAREVIAAVKPRLGGAESHAAEAFAALLLATGLAAPSLPAAQTAAPIAAPPATTRI
                     APVRTIPSVSADRAQQPMAARQRPAVAAAPTLAEDAEGDVQPLVAPPPGVVVVGKTLR
                     LPDAFDRSADAGTDIRSFAAYTEADDAGEAETLVFLIRMAGTVARSASFEVTWHFEGT
                     ACTADAVHDTYGSRTENDRARLAVTCPAEALIRMNPKDNHTTWYVDLVPTTRGDIVEV
                     RVPLRGLPHGLDAILHPGRTLTGLIATTYAATGLVMSDVDRAPDKDGVRYTIGTR"
     gene            complement(2484..2891)
                     /locus_tag="VM841_12980"
     CDS             complement(2484..2891)
                     /locus_tag="VM841_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91139.1"
                     /translation="MGRGVDLSTITRAERLVLIAGVLLFVNALIPYWYRIQTPRETFV
                     HNGGLYGYGLAAALAGFASAVIVIVRHFRTPAMFRDNAIHVMTGLIAAGALTAHASRS
                     DPLWIGFWVEAGLAAGLIVAGALRVRERKRGWI"
     gene            2945..3556
                     /gene="purN"
                     /locus_tag="VM841_12985"
     CDS             2945..3556
                     /gene="purN"
                     /locus_tag="VM841_12985"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HVL91140.1"
                     /translation="MSRAAPLRVGVLASGSGTLLQAIIDEQDESYSVAVVVSDRPNVA
                     ALERAERAGIEAVIVDYAGFDSRDAFSAAIAAALRHRSVELVCSAGFMRILSPAYFAA
                     VGVPTLNSHPALLPAFPGGHGVRDALEWGVKVAGTTIHFVDEEVDHGPIVAQEAIPVL
                     PDDTVDSLHERIKEIERRLYPEVIRGFAAGRFKVEGRRVHVIG"
     gene            3569..>4870
                     /gene="purH"
                     /locus_tag="VM841_12990"
     CDS             3569..>4870
                     /gene="purH"
                     /locus_tag="VM841_12990"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVL91141.1"
                     /translation="MPDVIRIQTALVSVSDRTGLSEFCKGLAEAGVTIIATDSTGRHL
                     AESGIATTPVAEVTGFPEMLGGRVKTLHPKIHGGILADRDDPKHLAQLTEAGIAAIDL
                     VVVNLYQFREAVAQDLESAQIIEQIDIGGPALIRAAAKNHTGVAVIVDPASYDAVLEE
                     ISMTGGLEFETRRALAAEAFAHTAAYDAAIARWMMRDEAFPEHLSIPMERVQALRYGE
                     NPHQGGALYAEVGGEGGVARAKQLHGKDLSFNNILDVDAAWETAQEFEEPAVAIIKHG
                     IPCGVAIGEDIATAYDGALESDRVSAFGGVVAVNRPLSRDAARKIAEIFIECVAAPSF
                     EPGALEVLMEKKNLRILQVIEPGLEPAMRRISGGMLVQDPDVFDDERGEMRVVTKTQP
                     TDEQWQDLLFAWRACKHVRSNAIVFAREGATIGIGGGQTSRV"
BASE COUNT          747 a         1663 c         1748 g          712 t
ORIGIN      
        1 catctcgccc ggcaaggcga acatggggat catccccgga gagatcaccg gtcccggtcc
       61 gatcgggctg ctgtcacgct cgggcacgct gacgtatcag gtgatgtacg agatgttcac
      121 gcgcggcgtg gggcagtcca cctgcgtcgg catcggcggc gacccgatcg tcggctcgaa
      181 cttcatcgac atcctcggcc gcttccaggc cgacgacgag acgcaggcga tcgtgctgat
      241 cggcgagatc ggcggcgacg aggaagagca ggccgcgcgg ttcatcgccg acaacgtcac
      301 caagccggtc gtcgcctaca tcgccggcca caccgcgccc cccggcaagc gcatgggcca
      361 cgcgggcgcg atcatcagcg gcagcaaggg caccgcgcag gccaagaaag aagcgttcga
      421 gtcgctcggc atcaaggtcg gcatcaaccc gaccgaagtc gccgagctcg cggtcaagag
      481 cctgtagcga gtacgaaagc gcgaaagagc cgcccgaatg tcccgggcgg ctctttcgtt
      541 cctcgcttct cgacatccca gccacagctt ccaactgtgg aggtcgagcg gcgcacgaat
      601 ggatcagtgc tctgcgccca acagtgccca tcagctggcg gctcggagcc agtctctcat
      661 cagggccggt cgagtcccat tagcgcgtgc cgatcgtgta gcggacgccg tccttgtcgg
      721 gcgcgcggtc cacgtcgctc atgaccagcc cggtcgccgc gtaggtggtc gcgatcaggc
      781 ccgtgagggt gcggccggga tgcaggatcg catcgaggcc gtgcggcagc ccgcgcagcg
      841 gcacccgcac ctcgacgatg tcgccgcggg tcgtgggaac cagatcgacg taccaggtcg
      901 tgtggttgtc cttcgggttc atacggatga gagcctcggc cgggcaggtc accgccagcc
      961 gcgcgcgatc gttttcggtc cgggagccgt aggtgtcgtg gacggcgtcg gcggtgcatg
     1021 ccgtgccctc gaagtgccac gtcacctcga agctggcgct gcgtgccacg gtcccggcca
     1081 tgcgaatcag gaacacgagc gtctcggcct ccccggcatc gtcggcttcg gtgtaggcgg
     1141 cgaacgagcg aatgtcggtt cccgcgtcgg cgctgcggtc gaaggcgtcc ggcaggcgga
     1201 gcgtcttgcc caccaccacg acgcccggcg gaggcgcgac gagcggctgc acgtcgccct
     1261 cggcgtcctc ggccagcgtg ggcgcggccg cgacggccgg ccgctggcgg gccgccatag
     1321 gctgctgcgc tcggtccgcg gacacggacg gaatcgtgcg gaccggggcg atgcgcgtcg
     1381 tggccggcgg ggccgcgatg ggagcagcgg tctgcgcggc gggcagcgat ggcgcggcca
     1441 gcccggtcgc gagcagcagg gcggcgaagg cctcggcggc gtgcgactcg gctccaccga
     1501 gccgcggctt cacggccgcg atcacctcgc gggcgcggcc ggcgatggag tggagcaccg
     1561 gcgccgcccc gaccggcagg agcgaatggg cggccagggc cgtgatttcg tcggcgacgc
     1621 tcgagcaagg cgagcagccg gcgtgcggcg cgggaatgcc gtccgatgcg gccttggcga
     1681 gccgcgacgc gcacgcgccg tccacgccgt gatgctcggc caggacccgg acggtctcga
     1741 cgcggagccg cttgacggcc cgatgcacca ccacgcggga ggcggccacc gaggttccga
     1801 gcgtggcggc gatctcctgc ggcgagcaat catcgacgaa ccgcagcagc acggccgcct
     1861 ggtcgcggtc ggacagccgc gacatcgccg aggcgacgag cgaggcgagg cgctcgtcgg
     1921 agacggcggg ctcctcgaag tcctcgacca gcatcaggtg ccggcgcgcg gcgcgctggc
     1981 ggtcgcgcac caggttgagc gcgcggtttc gcgcggtggt gagcagccat gccagcgcgg
     2041 cctgccggtc gcgcggcccc cgctcgagca tggtcaggaa ggtctcggac gcgatgtcgc
     2101 tcgcgtcttc gcggtcgcgc aggattcggg ccgcggcccg ctcgacggct ccccggtagc
     2161 ggtgatgcgc gtctgcgacg gtctccggtg gcgtcatctg tctccccctg gttcggatct
     2221 cgatactccc cacgtgtgag gacaccgatg tccgggctgc gttacagctc gcttgctcgt
     2281 ccgccaattc gacccatgcg gagcccattc cttgcgccga acccgcgaga ccgctcggag
     2341 tgctgatttc gcgtccgcgg ccccgggtcc tttcggccgg tatcgttttc tccgccgaat
     2401 gccgattccg cgggacgagc cggaaggcgg cggcgccggg agggcaggtc gagcaagagc
     2461 ccggatgcgc ggccatccga acgctagatc cagccgcgct ttctctcgcg gacgcggagc
     2521 gccccggcga cgatgagccc cgctgcgagc ccggcctcaa cccagaaccc gatccacagc
     2581 ggatcggagc gggatgcatg ggccgtcaag gccccggccg cgatgaggcc ggtcatgacg
     2641 tgaatcgcgt tgtcgcggaa catcgcgggc gtgcggaagt ggcgcacgat cacgatcacg
     2701 gccgaggcga aacccgccag cgcggccgcc agtccgtagc cgtacagtcc gccgttgtgc
     2761 acgaacgtct cgcgcggggt ctggatccgg taccagtagg ggatcagcgc gttgacgaac
     2821 agcagaacgc ccgcgatcag aaccagccgt tccgcgcgag tgatcgtcga cagatccacg
     2881 ccccgtccca cgcgtccgat tctacggact ggcgcccctt cgagggcctc gctagggttc
     2941 ggccgtgagc cgagccgcgc cgcttcgcgt cggagtcctg gcgtccggat ccgggacgct
     3001 gctgcaggcc atcatcgacg agcaggatga gtcgtactcg gtggcggtcg tggtcagcga
     3061 ccggccgaac gtggcggcgc tcgagcgcgc cgagcgcgcg ggcatcgagg cggtcatcgt
     3121 ggactacgcc gggttcgact cccgggacgc cttcagcgcg gcgatcgcgg cggccctgcg
     3181 ccatcggtcg gtcgagctgg tctgcagcgc cggcttcatg cgcatccttt ctcccgcgta
     3241 cttcgccgcg gtcggcgtgc ccacgctcaa cagccatccg gcgctcctgc ccgcattccc
     3301 cggcgggcac ggcgtccgcg atgcgctgga gtggggggtc aaggtggccg ggaccaccat
     3361 ccatttcgtg gacgaagaag tcgatcacgg cccgatcgtg gcccaagaag cgatcccggt
     3421 cttgccggac gataccgtgg attcgctcca cgagcggatc aaggagattg agcgccggct
     3481 ctaccccgag gtcatccgtg ggttcgccgc cggacgcttc aaggtcgagg gccgccgggt
     3541 ccacgtgatc gggtaggatt ccgggccgat gccggacgtc attcgaattc aaaccgcgct
     3601 cgtttcggtc tcggaccgca cgggcctgtc ggagttttgc aagggcctgg ccgaggccgg
     3661 cgtgacgatc atcgcgaccg actcgaccgg ccggcacctg gccgagtcgg ggatcgcgac
     3721 cacgcccgtt gcggaggtca ccgggttccc cgagatgctc ggaggccgcg tcaagacgct
     3781 gcacccgaag atccacggcg ggatcctggc cgatcgcgac gaccccaagc atctggcgca
     3841 gttgaccgag gccgggatcg cggcgatcga cctggtcgtg gtcaacctgt accagttccg
     3901 cgaagcggtc gcgcaggacc tggagtcggc tcagatcatc gagcagatcg acattggcgg
     3961 gccggcgctg atacgtgccg cggccaagaa ccacaccggc gtcgcggtca tcgtcgatcc
     4021 ggcctcctac gacgcggtgc tcgaagagat cagcatgacc ggcgggctcg agttcgagac
     4081 tcgccgcgcg ctggccgccg aggcgttcgc gcacactgcc gcctacgacg ctgcgatcgc
     4141 gcgctggatg atgcgggacg aggcgttccc cgagcacctg tcgatcccga tggaacgcgt
     4201 ccaggcgttg cgctacggcg agaacccgca tcagggcggc gcgctgtacg ccgaggtcgg
     4261 cggcgaaggc ggcgtcgcgc gcgcgaagca gctgcacggc aaggaccttt cgttcaacaa
     4321 catcttggac gtggatgccg cctgggagac cgcgcaagag ttcgaggagc ccgcggtcgc
     4381 gatcatcaag cacggcatcc cgtgcggcgt cgcgatcggc gaggacatcg ccaccgccta
     4441 cgacggcgcg ctcgaatccg atcgcgtgtc ggcgttcggg ggcgtcgtgg cggtgaaccg
     4501 gccgctgtcg cgcgacgccg cgcgcaagat cgccgagatc tttatcgaat gcgtcgcggc
     4561 tccctcgttc gagccgggtg cgctggaagt gctgatggag aagaagaacc tgcgcatcct
     4621 ccaggtgatc gagccggggc tcgagccggc gatgcgccgc atcagcggcg gcatgctggt
     4681 tcaggatccc gacgtcttcg acgacgagcg cggcgagatg cgcgtggtca ccaagacgca
     4741 gccgaccgac gagcagtggc aggacctgct gttcgcctgg cgcgcctgca agcacgtgcg
     4801 ctccaacgcg atcgtcttcg cgcgcgaggg cgcgacgatc ggcatcggcg gcggccagac
     4861 cagccgcgtg
//
LOCUS       DATLFO010000321        28944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_137099, whole genome shotgun sequence.
ACCESSION   DATLFO010000321 DATLFO010000000
VERSION     DATLFO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 28944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28944
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137099"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VM841_12995"
     CDS             complement(<1..985)
                     /locus_tag="VM841_12995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL91142.1"
                     /translation="MGTTAQERTDRLNDKIFAAGLGATDLTILYLGEKLGLFRAIAAA
                     GSATPAELAKATGTAGRYCREWLEAAAATEFLEVDDPAAAPDERRFRIPAGGEALLAD
                     PADPSFQGYVPMLFTGCIVMLPAIVEAFRTGGGVPWSQYGPDVIDGQEAQNRNLYLSS
                     LATEWLPKIPDVHARLSEPGARVADVACGTGWSSIAIAEAYPGAEVHGFDLDEYSIAK
                     ARGYAEERGLAGRVRFEVRDAADPSLSGAFDLVLILEALHDMGDPVAVLRATRSLAGD
                     RGMVVVMDEKTEETFTPNAPDLERLFYGFSLLACLPAALADGPVGTGTVMRP"
     gene            complement(1066..1536)
                     /locus_tag="VM841_13000"
     CDS             complement(1066..1536)
                     /locus_tag="VM841_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91143.1"
                     /translation="MTIRSTVEGISPDDVLVFLTGLPEAFGYETVIKPLRYRSRPHLS
                     AYTEFDHKRITLQVPMPFFPFGEIVHYGAKRMPAKGMRFVWLSEGLTFREPREVLRFL
                     WCHEWYHWFLYEVLGRKSAAETACDRFALRNYLKPVVTLEDAHAALRRSPAETG"
     gene            complement(1569..2609)
                     /locus_tag="VM841_13005"
     CDS             complement(1569..2609)
                     /locus_tag="VM841_13005"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVL91144.1"
                     /translation="MRPFRLAAALLALCLVASACGRIEATAGRARPLGCVEPNKRMHL
                     YAEQLPDAADGSIRLGWGLSPGTATIPGPTIELTEGDCVAITVVNDIPVATLAALRDD
                     PRLGSGNPSQPLGVSLHVHGVKYTQDSDGTLETDSWVRPGQSRTYTWYAAPRVSVGKR
                     VASLGTAGYWWYHDHIIGTSHGTGGIGSGLVGGLVVRRPGDPEPDRPTNVVVMGPDQT
                     INFQRYPNTPRFVARRGERVEFLVIGVGDEFHTFHLHGHVWADTRTGFFATQEDETQL
                     IDVRTIGPSETFGFQVIAGESVGAGSWMLHCHVQVHSDRGMVTFFDVLEPNQPLPVAP
                     IAPANGGHHLHG"
     gene            complement(2698..4128)
                     /locus_tag="VM841_13010"
     CDS             complement(2698..4128)
                     /locus_tag="VM841_13010"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVL91145.1"
                     /translation="MNGERNGLSWHRRALTALLLACAAAASLALRVAPVAAAPQAHPV
                     EVRSNFFEPRVINIAPGDTVTWTAVEFGHTVEADNGRFNFYPQRYIHRGESVSFTFLT
                     EETVRYHCRVHGSSGGGGMSGTIIVGAGSPSPTPTPTPSVTEHRYVPTPEYKTIGAAL
                     NGIAPGGVVHLAPGEYRESVAVRTPGVTVQGDGAPSDVVIDGQGLRRTGVLLAAGGTA
                     VRSLTVRGHRAEGIAVRGVTVASVRNVASHGNGSYGVLVADASGVTVEDVAATGHRVA
                     GIAVQGCDECDTVVQDVTVMQNLYGVLLDNAGSVVVRRSTIAANATGIVARSLATGDG
                     TVQRGVHITGNDITGNTTAIPAASATEFAVQSGVWLAGVWFALVDDNRIDGHDYGVLV
                     TSFGVPSLDGRISGNVIGGSAVADLGWDGVGAGMCFSGNAAAGGGALATRPSFAETVY
                     VCGGTRLPGIPEPLVIADLARDAAGG"
     gene            complement(4210..6363)
                     /locus_tag="VM841_13015"
     CDS             complement(4210..6363)
                     /locus_tag="VM841_13015"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HVL91146.1"
                     /translation="MHRRRALVPLAAVLALLGTLIAGGSVPAAAASPSTMGQFLPAFS
                     ENGHFDAAPPSNVSESTRYPTAVSLAAMPNGKLVYWNGLENLESDRYPLATDANRISP
                     GSRSRQLAFDANGAPIFQVPTPEDAGEDPGDPDDQGDLFCADQRLLLDGTVAAIGGTV
                     WKPGPVDLVKLSNGAITEPQGTTELHGNNATRIFSSDGNGGTWTPAERMNYGRWYPAV
                     VTLPDGRLFVAGGVKQLIYNDSGLNVHQTEIFDPYDADGTYAPAQGEWTENGASGETS
                     LPLFPRLHVIHDGTVLYDGVGQMWGPFGQAVDEALWNVVKSYDPATNSWTDRGVGAYG
                     ALSGAVSVMLPFTPDANGTYAESKILHAGGVLGTSPGAFVATDLSEVMTIGADGSLTA
                     TPGPRLNNRRWYSQGVIGATGDPLIFSGADKDEVIAPGFEAPVRQAEMYDVATGQFVP
                     LASAARDRTYHNSAVLLADGRILIGGHAPINTLYGPGEQEGYTSNDMHNNTGLTANNL
                     KDSSFEIFEPPYLHRGPRPSITSNVSGIMEWGTERSITTPDAATVDKAILVKMPATTH
                     VTDPDARAIEVRITERLSGAVKIAVPDNAAVAIPGYYYLFLTRTNAGEAGPTVSNGIV
                     VRVGSEAVFGGASADIAPVQLPQQTLTGSTDAGVTSGPAAAIGSAVVEPASMRATGSR
                     EARTGGRGGLLAVAAAAMTAATGAWLRRRRVAVRT"
     gene            6664..7476
                     /locus_tag="VM841_13020"
     CDS             6664..7476
                     /locus_tag="VM841_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91147.1"
                     /translation="MAASFAASRRMPLAATAIVLGIALLTARAGSAGTVCEPGGGGTP
                     VPSASPAPTPTIPLGVLGGTAASREQAASTGTCDAAVGLDVAPAGGGVWGTTFGLSGS
                     LSCNGNLLGGKQVTLQKLSGGSWTAVSSGPTDDSGYTFTQKPSHSGTYRVAFAGDELC
                     SPAESPAKTVIVRPGVAFNAASGATSRRGGTAIFSGSVLPAHPGHVVTLQVLQSGAWR
                     NAVTVKLDSQSRYRLAYTRASGSGPLLFRVAYPTQHADHGWNVSRSIRVTWS"
     gene            complement(7661..8401)
                     /locus_tag="VM841_13025"
     CDS             complement(7661..8401)
                     /locus_tag="VM841_13025"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HVL91148.1"
                     /translation="MREPTIDLNADVAEGADDAALYSIVTSVNIACGAHAGDDETMRD
                     AVARAREHGVAIGAHPGYPDRDSMGRRALAIPPQEIARLMVEQIAALDRIVSRAGLRI
                     RHVKPHGALYNQAAADAAIARAIASAVAAFDPALTLVGLAGSEMLAAGRAAGLEVAGE
                     AFADRGYRADGTLLPRDEPGALIQDPVRAAAQAVALARGEPVPAGDGSVVVEADTVCL
                     HGDTPGAVRNAVSVRAALEAAGIKVAPL"
     gene            complement(8394..9293)
                     /locus_tag="VM841_13030"
     CDS             complement(8394..9293)
                     /locus_tag="VM841_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HVL91149.1"
                     /translation="MMRLTRAGFSSVQDLGRAAPGASGVPRGGAFDEWALRRGNRLCG
                     NEPDAAGIEFVLAGPEVVFEKAALVAITGAPFAATLDGAAVEGEIAVPVAAGATLSIG
                     KAARGARGYLCVAGGIATEPVLASRSASPRAGIGLMLAGGDRLPAGTASRGGPERPRP
                     NRPETPDATPDAIRVVAGPSDLAEDSLERLIEEEWTVTPDADRVGVRLDGPALAHGPG
                     GPEIDPEPAMPGVIQVPGDGRPIVLGPDGPATGGYPKIATVIRADLWVLAQARPGDRL
                     RFRLCDPAAARAALAEQAGRTDA"
     gene            complement(9290..9895)
                     /locus_tag="VM841_13035"
     CDS             complement(9290..9895)
                     /locus_tag="VM841_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HVL91150.1"
                     /translation="MWRWQGESAIVVRFDGPLIRANERARAAAAAAATREDVLAAVPG
                     ARTVLAHLHDGADPAAVDAALRAAPPVAAGEIREHRIEVVYDGEDLVALGAERGLAPR
                     EIVALHTGATYTVAFLGFMPGFAYLLGLPEPLYAARLATPRTRVPAGAVAIAGVWAGV
                     YPSASPGGWRLLGRTDASLFDASAVPPAMLAPGDVVRFAEA"
     gene            9912..10745
                     /locus_tag="VM841_13040"
     CDS             9912..10745
                     /locus_tag="VM841_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HVL91151.1"
                     /translation="MPELPEMRALAERLEAVVGGAALARIEPIHFSALKTVTPDPSSL
                     AGAPVEGIGSRGKYLLMRVPGARIAIHLSQGGRVDVEDPPKSTRPKGGVVRFRFERAP
                     SILIKEFGTERKAGWWVLAAGDDGPMASLGPEPFTDEMRSLIVGGEDKRRLHTMLRDQ
                     RTIAGIGRGYADDILHRAMLSPFASLSSLDEAKRTTLLDAVHEVLEQGLAAERRRTGG
                     LPPKLGGHWIVHGRFGEPCPRCGERIERVSYESHEVAYCPACQTGGKVLADRRMSRLL
                     K"
     gene            complement(10746..11099)
                     /locus_tag="VM841_13045"
     CDS             complement(10746..11099)
                     /locus_tag="VM841_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91152.1"
                     /translation="MRRVAVTALLALAACSNADAAPDQGTVRTCFNTIGVKEPDADTL
                     GSVQGWVNVAEQAVAEAKHEQVRTAGEALKAALSPQVQADGFDPEEVERYAMAVARMQ
                     KACKDSNAFKGNPRA"
     gene            complement(11096..11899)
                     /locus_tag="VM841_13050"
     CDS             complement(11096..11899)
                     /locus_tag="VM841_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL91153.1"
                     /translation="MTDRYAAYSSLRIEQAEPHILRLVMSAPGKLNAASADMHRELAG
                     VWLEIDRDPDTRVVIVTGEGKAFSAGGDLDLVKEMAQDFEVRARVLKEARDLVYNTIN
                     CSKLIVSAMRGPAVGAGLVVGLLADVSIASTTARIIDGHTKLGVAAGDHSAIVWPLLA
                     GMAKAKYYLLTCEPLSGEEAERIGLVSMCVPDDELEHTAMSVARRLAAGSQTALRWTK
                     LALNNWLRSAGPAFDASLALEFMGFAGPDVHEGIAAVREKRPPRFGEQP"
     gene            complement(11917..14712)
                     /locus_tag="VM841_13055"
     CDS             complement(11917..14712)
                     /locus_tag="VM841_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HVL91154.1"
                     /translation="MAAAAASLIVVAMAGVLAPLPTANAFPVAADDARYLAYGRVFPD
                     PHGCLRGLPGARPWAKGNVCAVQFLQWQDVLDGLGYLESRFEGMIELINLREKFVTDP
                     DFLEDELQSAGIPQEDLSRAKRDLHVIKVTDASSKVPEKDRKHFAYSLSIHGIERAGI
                     EGGIRAAEDLVTWAACEKSADASPACANEGVSPERPKRLLDAFDPPEGPTAGEVLNKA
                     VIYFVLSNPDGWSRGEVTKGNVFFQRYNGNGIDVNRDWPPQGYSQPEYTPGSEPETRG
                     YAKFLRKMRAKTSAGAFAGSIDLHGMVTSHAFSFTMLGSAQRDYRKNAITVQTAITTW
                     RDAEERLTWSPLVARNDSCPGPLPEPFFGGQHGPMCTDLWGTVWDMIRYQVTGSFGDW
                     MDSPLGLDAVGVSNEMAYSHLAPNTAFDPGIEQLHVDGNKGLIYSQIASLLDEKPVRY
                     APKGKVGYVFDPARIRNAGKAAVASPLANLKPQPPISLDMPNGSDEVTFDVKGPSDGI
                     FNGGMTVEATFSNVRSMSADGLLTEGFIIDYCGPPDHFDDPEGCREAGRYWNQSATYA
                     QAGARVDINNPKPGPYRIRPDDGRIGPSKVTVRFTPGAATPGGQMPYDGSRMDFFKDL
                     NRYVPAGKKLEPVTVEQILATPGMLHGFDSVGSAPGSRFDGAGRQRYSSALRAFAAGG
                     GALVLTDSALSGLPLLNPQIAPAKVGRGYFYAGYMDFDDGDGPTYERHPLAKDVNKPG
                     ASEGFDTLGGVEYDNRHQTCEPGPLGYYISPRGSSNDNCQIDRCDSPNWIVDEETWRE
                     AGGSVAARTFVHTQPDPASDQTPWRAGVSLGDLPLGKGVVRIAGAMLPDASEQHYRPF
                     GLSSYALTYTGYQVFENALAWSRTHKPAPATVLPAKKRAPLPATGVGATAPFVIALGG
                     LALAAGAWTRRGGATRG"
     gene            14804..15919
                     /locus_tag="VM841_13060"
     CDS             14804..15919
                     /locus_tag="VM841_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HVL91155.1"
                     /translation="MKFVLRPRVRTAARIVLLQRLLFAGAGLLIAWFLAPGLGRLTDG
                     VLDVWDRLDASRFARIAEHGYDNPDDPHAAAYFPLVPMVMAAARAMRIEPIAGAMIVN
                     TVAALVAFVYLYRLVEEEFDAETARRALWYLAFFPTAVFLVAPYSEPLFLAGAIPAFY
                     YARRQRWHLVGLPAAIACTARFAGVFLLIGLIAEFLRQRSWRPRRVATAAVALATGAL
                     PIAVFFLYLWRVTGSPARFFEAQRDGWGRIFVGPVRSFGTTWETWTGSHFFTNQLIAW
                     RVEIVAAALGVALVVWAALKREWGYAAYMGSMHAALVTSSWYYSIPRAMLTMFPAVIF
                     LAAHTVRKPKRHEPLLAVFASIAMFGVIVYTRGHWFF"
     gene            complement(15916..18354)
                     /locus_tag="VM841_13065"
     CDS             complement(15916..18354)
                     /locus_tag="VM841_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91156.1"
                     /translation="MAVLLALAAMMPGSGVRAAAGDPVVEDAEWILTLQVPSGPHAGL
                     IAMNEPRSLCIPYFANMAASGLASATLSSGDARYAEAAWRWLDWYAAHMDASGFVTDY
                     ELSGGSWVSTGDYDSTDAYAATFLSAVRAAYAATGDAQRLAAIWPAVRRATDAMLATA
                     APGYLTYAKPGWNLMYAMDNAEVYEGWIAVAELALVHGDAALRARADEWIGGMRVSLA
                     SFWNEAAGAYDVALGSGGVRHPADWSEFYPSVTAQVWMINTGLVPAARAASLAGRIDR
                     ENPSWDDPWSPGLFNPPEGPQVRAIGWWPSFADAFTEAGMQSRALAAFTRMRDAALAG
                     GRSWPYHAGSAGRMIDFVVARPDTAITGGPSAWTSEQAATLAFAGSPSAAAFECSLDG
                     EPWAPCVSPRSWTGLGDGPHVVRVRALDAAGHADPIPASRSWIVDTVAPSVRLLSAPP
                     DPGGIGSAVFAFDGADDRTAAGVLVHECSLDGAPYATCVSPKAYPAIATGSRSFRVRA
                     RDLAGNVSLAATHDWFADSVAPETVITSGPSGPVTEERATFAFSATDDAPGPLWFECR
                     LGAAAFSACASPVSYTFAHEGMFVFSVRAVDGAGNRDATPASRTWFVDRTPPETTLGA
                     GPAAFTASRDARFTFSGSDSVSPPHWLMYECSLGGAAFSRCTSPVNLTGLADGEHVFE
                     VRAVDASGLADPTPARLEWTIDTAPPRSVIVTPDQAVIAHLPPAMERFVRGTVTDDRS
                     GLREVAVTFVPVGAPGVTVVATISCEDASARSCTWAAAAPAGRGRYEVRVVATDRAGG
                     AESPGPKPIAVLVV"
     gene            18500..19309
                     /locus_tag="VM841_13070"
     CDS             18500..19309
                     /locus_tag="VM841_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HVL91157.1"
                     /translation="MTEQPKDPTHPHFGEEGRRLSYGTYLKVPELIDLQLPLARPEAH
                     DELLFIVVHQVYELWFRQLLFELDAIREHLFAGRAPQARHLLERVHEIERVLVAQVAV
                     LETMTPSDFMDFRRNLAPASGFQSVQFREIEFVSGLKDEGVIARLDPQAGEAARLKRR
                     LGEPTLWDAYCELLTSRGLPMPDDDADRRRDSLLRMTRERDRFVDEHAIGEALLTHDE
                     LFALWRHRHVLMVERQIGTKSGTGGSAGAPYLRSTLTRRFYPELWDLRSYL"
     gene            19485..22181
                     /gene="aceE"
                     /locus_tag="VM841_13075"
     CDS             19485..22181
                     /gene="aceE"
                     /locus_tag="VM841_13075"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HVL91158.1"
                     /translation="MIIDGFAHQVPDIDPGETEEWLAAFDGALDARGKARARYLLVKL
                     LERARERGVGFPATVSTHYLNTIPAHLEPEFPGDEHMERRIRAFIRWNAAIMVVRANL
                     INAGIGGHLATYASSAALYEVGFNHFFRGKDGGTYGDQVFIQGHASPGIYARAFLEGR
                     LTEEQLDHFRREVNGGGLSSYPHPRLMPDFWEFPTVSMGLGPLSAIYQARFNRYLLNR
                     GLVDTSASKVWCFVGDGETDEPETLGALSIAAREKLDNLIFVVNCNLQRLDGPVRGNG
                     KIIQELEAVFRGAGWNVIKVIWGRKWDELLAKDHDGVLVAKMDSTVDGEFQRYATETG
                     AYIREHFFGPDPRLRAMVEHLSDEDLRLLPRGGHDYRKLFAAYKHAVEHEGTPTVILA
                     KTIKGWTLGPDVEGRNVTHQVKKLTQEQLFELRERLFLHEEVPESALQQELPPYYRPE
                     PESDEYAYLMERRRALGGSLPKRVVRPEQAETPAPKGEVFDDLTTGSGTQEASTTMAF
                     GRLLRDMLRDKGFGRFVVPIIPDEARTFGLDALFRQVGIYAPHGQLYEPVDAHLLLSY
                     TESKQGQILEEGITEAGSMASFIAAGTSYATWGEPMIPFFIFYSMFGFQRIGDLVWQA
                     GDARARGFMLGATAGRTTLNGEGLQHEDGHSLVLASTVPNVMAYDPAFAYEVATIIRN
                     GIDRMTGKDAEDLIYYLTLYNENYPQPPMPEGAAEGVLRGLYKFAAAPNGGKRRATIL
                     FSGTAWKAAMEAQRILSKDWDVAADCYSATSYKCLRENALGAERWNRLHPTETPRVPY
                     VTQVLAEAASPTVAVSDFMKVVPDQIARWAPQPFTPLGTDGYGRSDTRETLRRHFETD
                     AAHVVVATLHTLAQAGDAKPQEVMDAIGRYDIDPDAIDPLHA"
     gene            22453..23427
                     /locus_tag="VM841_13080"
     CDS             22453..23427
                     /locus_tag="VM841_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HVL91159.1"
                     /translation="MHVEPWMWIAFTAFILAMLAIDLLMNRDAHVVSMREAGIWSGVW
                     VTLGLLFAGLLWIWQDGKVAGEYLAGYVIEKSLSVDNIFVFALLFSYFSVPAKYQHRV
                     LFWGVLGALVFRILFIAGGAALLDNFHWMIYVFGGFLVITGIRMAMHKETEVHPEKNP
                     VLKLMRRVIPISGSYDGKHFFTRVNGKRLATPLFAVLVVVETTDIIFAVDSIPAIFAI
                     TRDTFIVFTSNAFAILGLRAMYFLLAGMIRKFQYLSVGLSAVLVFVGIKMLISDVYHV
                     PIWASLSFIAVAITASVIYSLRHPAAPAEIPHGEGLQELREDAQEMPR"
     gene            complement(23409..24206)
                     /locus_tag="VM841_13085"
     CDS             complement(23409..24206)
                     /locus_tag="VM841_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVL91160.1"
                     /translation="MSSEPMTITRNRALLMLWDAWIVTKRNLIRYQRVPTLLVFSTIQ
                     PVMFTLLFRYVFGGAIDPGDGIAYVDYLMPGVFGQVVVFGSTTTGVALAQDLTYGMID
                     RFRSLPMARSAVLAGRTLSDAVRNLFVVMLLLLIGTLVGFRIHTGVAPAVAAILLAVL
                     FGHAFSWISAFIGLSVRDVESTQAASFVWVFPLVFASSAFVPVASMPGWLQAWASVNP
                     VSVTVDAMRGLLVAGPALVPAVKSLAWIAALLLVFVPASVRRYRGIS"
     gene            complement(24203..25210)
                     /locus_tag="VM841_13090"
     CDS             complement(24203..25210)
                     /locus_tag="VM841_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVL91161.1"
                     /translation="MEAAVVAEGLAKRFGEVEALKGVDLRVAAGSVLGLLGPNGAGKT
                     TTVRILTTLLVPDAGRATVGGYDVVRDADRLRHVFGLAGQYAAVDDTLTGRENIVMVG
                     RLYHLRRAEAERRAGELLERFSLSFAADRVVKTYSGGMRRRLDLAASLAGRPKILFLD
                     EPTTGLDPRSRIELWEMIDELVGEGMTLLLTTQYLDEADRLAHRIAVIDDGRVIAEGT
                     SDELKHRVGGDVLELHIEDAAKVEAAAKRIADLGSGEPLVDALRRRVTLPIAAKGPKI
                     ITEVVQRLGRSRIELADFAVRRPTLDDVFLRLTGHEATQAEEARVAAGGRRGRGRKRT
                     A"
     gene            complement(25279..>28944)
                     /locus_tag="VM841_13095"
     CDS             complement(25279..>28944)
                     /locus_tag="VM841_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HVL91162.1"
                     /translation="MLDNVLELLAREGQYPGESRDIRHAVAMLVPAAWEQNASMLREV
                     RDFYRWHASLMEPWDGPAGLIFTDGVRVGAALDRNGLRPLRTHVCEDGFVAVCSEAGA
                     IDVSGHGRVKRGKIGPGEMVFVDPADGGFQTDPIRRIAGRQPYGAWLSEYRTVQSPGD
                     AVIEVPEDLVRRQVAHGYTREETSLILRPVAANGKEPTFSMGDDTAMAAIASHRRTLY
                     GYFKQRFAQVTNPAIDHLRERAVMSLRTLIGPQDPLLWERPEACAHLEYDSFLLFKPP
                     GGAVIDATFDPNEGPGALRGALERIAAAAVRAASSGSGILVVSDAAAGPSRAPIPSLL
                     ATGAVNTALLRSGHRTRCSIVVQTDDARESHHFACLLGYGAEAIYPRLALATVASLAS
                     SGRARDVGSPDEAMRQFKIAIEEGVLKVLSKMGISTVDSYRGAAIFDALGVGAGVIEL
                     CFEGTVSPLGGIGFDEIQADVLERHAAAYGAHDKKPVLANPGFVKFSSKGEQHTNSPS
                     VVRALHRTVDPTGEILTSTQSGEDGDDAPGEDEIAAKTLQAAVRGEPNAELYERFAAM
                     VNARPPAAPRDLLDLVAAPESVPLEEVEPAEAILRRFSTGAISHGAISKEAHETLAEA
                     MHLIGGRANTGEGGEDPARYRTSKNSAIKQVASGRFGVTPEYLAFAEEIQIKIAQGSK
                     PGEGGQLPGHKATEEIARLRHTQPGVALISPPPHHDIYSIEDLAQLIFDLKQANPAAD
                     VSVKLVAEAGVGTIAAGVIKGLADIVHISGADGGTGASPLTSIKNAGMPWEIGLAEAQ
                     QALVENGLRTRARLRVDGGFKTGRDVIVAALLGADEYSFGTAVLLAEGCIMVRTCHLD
                     TCPVGIATQRPELRAKYAGTPQMVAAYLRFVAEETRRYLAVLGLRSLDEAIGRTDLLR
                     VRADAGERALLLDLTPLIGAAATAAATPRRFVEPVPTMRPRDALGHRIAGDAFDAIRA
                     GERIELRYAIGNGDRTVGATLGGMIGREFGAAGPPGRARIELTGQAGQSFGAFLAAGV
                     ELILYGEANDYVGKGMGGGLIAIVAPGDDAGDPYLAGNTVLYGATGGTLLVAGRAGER
                     FAVRNSGATAVVEGTGDHACEYMTGGTVVVLGPTGANVGAGMTGGEAFVYDPDASLPA
                     RVNPDLVDVRKPGDEALARVKALIERHMAVTGSARAAEILAGWERASAAVRRIAPRED
                     VAARSSKAEGTVKAGKA"
BASE COUNT         4244 a        10266 c        10096 g         4338 t
ORIGIN      
        1 ccggtcgcat caccgtcccg gttcccacgg gtccgtccgc cagcgccgcg ggcaggcacg
       61 cgagcaggct gaacccgtag aagagccgct cgaggtcggg ggcgttgggc gtgaaggtct
      121 cctcggtctt ttcgtccatc actacgacca tgccgcgatc accggcgagc gatcgcgtcg
      181 cgcgcagcac cgctaccggg tcgcccatgt cgtggagcgc ctccaggatc agcacaagat
      241 cgaacgcacc cgacagcgac ggatccgctg cgtcgcgcac ctcgaaccga acgcggccgg
      301 cgagcccgcg ttcttccgcg taaccgcgcg ccttcgcgat cgagtactca tcgaggtcga
      361 acccgtgcac ctcggcgccg ggatacgcct cggcgatcgc gatcgaggac cagccggttc
      421 cgcacgcgac gtcggcgacg cgcgcgcccg gctcgctcag ccgcgcgtgc acgtccggga
      481 tcttcggcag ccattcggtc gcaagcgagg agagatacag gttgcggttc tgcgcctcct
      541 gcccgtcgat cacgtccggc ccgtactgcg accacgggac gccgccgccg gtccgaaacg
      601 cctcgacgat cgcggggagc atcacgatgc agccggtgaa cagcatcggc acgtagcctt
      661 ggaaggacgg gtcagccggg tcggcgagga gcgcctcgcc gccggcgggg atgcggaagc
      721 gacgctcgtc gggcgcggcg gccggatcgt cgacctcgag gaactcggtc gcagccgcgg
      781 cctcgagcca ttcgcggcaa tatcgtccgg cggtgccggt cgccttcgcg agctcggccg
      841 gcgtggccga tcctgcggcc gcgatcgccc ggaaaaggcc gagcttctcg ccgagataca
      901 ggatcgtcag atccgtcgcc ccgagcccgg cggcgaagat cttgtcgttg agccggtcgg
      961 tcctctcctg tgcggtcgtc cccatcggtg gtctccctct gtcgatccgt cgatgtctct
     1021 cgccattcga ccgcagggcc gcccccggcg tccagcgcgc cccggctagc cggtttccgc
     1081 gggtgagcgg cgaagggcgg cgtgcgcgtc ctcgagcgtc acgaccggct tgaggtagtt
     1141 ccgcagcgcg aagcgatcgc aagccgtctc ggcggcgctc tttcggccga gcacctcgta
     1201 gaggaaccag tggtaccact cgtggcacca caggaagcgg agcacttcgc gcggctcgcg
     1261 gaacgtgagc ccttccgaaa gccacacgaa ccgcatcccc ttggccggca tgcgcttggc
     1321 cccgtaatgg acgatctctc cgaacggaaa gaacggcatc gggacctgca gggtgatccg
     1381 tttgtggtcg aactcggtgt aggccgacag gtgcgggcgg ctgcgatagc gcagcggttt
     1441 gatgacggtc tcgtaaccga atgcctccgg caggccggtc aggaaaacga gaacgtcgtc
     1501 gggagagatt ccctcaacgg tggagcggat cgtcatgacg ccgtcgagcc taccggcgtg
     1561 gtgatacgtc agccgtgaag atggtgaccg ccgttcgcgg gcgcgatcgg cgcgaccggc
     1621 agcggctggt tcggttcgag cacgtcgaag aacgtgacca tgccgcggtc ggagtggacc
     1681 tggacgtggc agtggagcat ccacgagccc gcgccgaccg actccccggc gatcacctgg
     1741 aagccgaacg tctcggacgg cccgatggtg cgcacgtcga tcagctgcgt ctcgtcctct
     1801 tgcgttgcga agaacccggt tcgcgtatcg gcccacacgt ggccgtgcaa gtggaaggta
     1861 tggaactcgt ccccgacgcc gatcaccaga aactcgacgc gctcgccgcg ccgggcgacg
     1921 aagcgcggcg tgttcgggta gcgctggaag ttgatcgtct gatcggggcc catcaccacg
     1981 acgttggtcg gccggtcggg ctcgggatcg ccgggccggc gcacgaccag cccgccgacc
     2041 aggccgctgc cgatgccgcc ggtgccgtgc gaggtgccga tgatgtgatc gtggtaccac
     2101 cagtagccgg cggtgccgag cgaggcaacg cgtttgccga cgctcacgcg cggcgcggcg
     2161 taccaggtgt aggtgcggga ctgtccgggg cgcacccacg agtcggtctc gagcgttccg
     2221 tcgctgtcct gcgtgtactt gacgccgtgc acgtgcagcg acacgccgag cggctgcgag
     2281 gggtttccgc tgccgagccg cggatcgtcg cgcaacgccg ccagcgtcgc gaccggaatg
     2341 tcgttgacga ccgtgatcgc cacgcagtcg ccctcggtca gctcgatggt cggcccgggg
     2401 atcgtcgcgg ttccgggcga caggccccac ccgagccgga tcgagccgtc ggcggcatcg
     2461 ggaagctgct cggcgtacag gtgcatgcgc ttgttgggct cgacgcagcc gagcggccgc
     2521 gcgcggcccg cggtcgcctc gatccggccg caggcggacg cgaccaagca cagcgcgagc
     2581 aacgcggcgg cgaggcgaaa cggtctcatt cccgtcgtgg ttcgcgccgg tgcggccgca
     2641 tccttcccgg gctcgctccc ccggtaccgc tgcatcccga tgcgtcgcat ttgcccctca
     2701 cccgcccgcc gcgtcgcgag cgagatccgc gatcacgagc ggctccggga tgccgggaag
     2761 ccgggtgccg ccgcacacat agaccgtctc ggcgaacgac ggccgggtcg cgagcgctcc
     2821 gccgccggcg gcggcgttgc ctgagaagca catgcccgcg cccacgccgt cccagccgag
     2881 atcggccacg gccgatccgc cgatgacgtt gccggagatc cggccgtcga ggctgggcac
     2941 gccgaacgag gtgaccagca cgccgtagtc gtggccgtcg atccggttgt cgtccaccaa
     3001 cgcgaaccaa acaccggcga gccatacgcc cgactgcacc gcgaactcgg tcgctgacgc
     3061 ggccgggatc gcggtggtgt ttccggtgat gtcgttgccg gtgatgtgca cgccgcgctg
     3121 caccgtgccg tcgccggtgg cgagcgagcg ggcgacgatt ccggttgcgt tcgcggcgat
     3181 cgtcgagcgg cgcaccacca cgctgcccgc gttgtccagc agcacgccgt acaagttctg
     3241 catcacggtc acgtcctgca cgacggtgtc gcactcgtcg cagccctgga cggcgatgcc
     3301 ggcgacgcga tggccggtcg cggccacgtc ctcgacggtc acgccgctcg cgtcggcgac
     3361 aagcacgccg tagcttccgt tgccgtgcga ggcgacgttc ctcacgctcg cgaccgtgac
     3421 gccgcgcacc gcgatcccct cggcgcgatg gccgcgaacg gtgagcgaac ggaccgccgt
     3481 cccgcccgcc gccagcagaa cgccggttcg tcgcaggcct tggccgtcga taaccacgtc
     3541 ggacggcgcc ccgtcgccct ggacggtcac gccgggagtg cggaccgcga ccgattcgcg
     3601 gtactcgccg ggcgccagat gaacgacgcc gcccggcgcg atcccgttca gcgccgcgcc
     3661 gatcgtcttg tactcgggcg tgggcacgta ccggtgctcg gtgacggacg gcgtcggagt
     3721 gggggtcggc gacggcgagc cggcgccgac gatgatggtc ccgctcatcc ctccgccgcc
     3781 ggagctgccg tgaacgcggc agtgatagcg gaccgtctct tcggtcagga aggtgaacga
     3841 gaccgactcg ccgcgatgta tgtaccgctg cgggtagaag ttgaagcgtc cgttgtccgc
     3901 ctcgacggtg tgcccgaatt cgaccgcggt ccaggtgacg gtgtcgcccg gcgcgatgtt
     3961 gatgacgcga ggttcgaaga agttcgagcg aacctcgacg gggtgcgcct ggggagccgc
     4021 ggcgaccggc gcgacgcgca gcgcgaggga cgcggcggcc gcgcatgcca gcagcagcgc
     4081 ggtcaacgcg cggcgatgcc agctcaatcc gttcctctcg ccattcatct cgtctgctcc
     4141 ctgatggtcg aaaggctcgc cctcgtgtga cacgggggcg agcccttcgc agctcgcgcg
     4201 cggctgccgc taggtgcgga ccgcgaccct ccggcgacgc agccacgcgc cggtcgcggc
     4261 ggtcatcgcc gccgcggcca ccgcgagcag gcctccgcgg ccacccgtgc gggcctcacg
     4321 cgacccggtg gcacgcatcg acgcgggctc gacgacggcc gagccgatcg ccgcggccgg
     4381 tccgctcgtg acgcccgcgt cggtggaacc cgtgagcgtc tgctgcggca gctgcaccgg
     4441 tgcgatgtcg gccgaggcgc ccccgaacac ggcctcgctg ccgacgcgaa cgacgatgcc
     4501 gttcgagacg gtgggaccgg cttcaccggc gttggtgcgc gtcaggaaca ggtagtagta
     4561 gccgggaatc gcgacggccg cgttatcggg caccgcgatc ttgacggctc cggacagacg
     4621 ctccgtgatc cgaacctcga tcgcgcgcgc gtcggggtcg gtcacgtgcg tggtcgccgg
     4681 catcttgacc aggatcgcct tgtcgaccgt ggcggcgtcg ggggtggtga tgctgcgctc
     4741 ggttccccac tccatgatgc cgctgacgtt cgaagtgatc gacggccgcg gaccacggtg
     4801 caggtagggc ggctcgaaga tctcgaagga cgagtccttg aggttgttgg cggtcaggcc
     4861 ggtattgttg tgcatgtcat tggaggtgta gccctcctgc tcgcccggac cgtagagcgt
     4921 gttgatcggg gcgtgaccgc cgatcaggat gcggccatcg gccagcagaa cggccgagtt
     4981 gtggtaggtg cggtcgcggg ccgccgacgc gagcggcacg aactgtcccg tcgcgacgtc
     5041 gtacatctcg gcctgacgaa ccggcgcctc gaagccggga gcgatgacct cgtccttgtc
     5101 ggcgccgctg aagatcagcg ggtcgccggt cgcgccgatg acgccttgcg aataccagcg
     5161 gcggttgttg agccgcgggc cgggcgtggc ggtcagcgag ccgtccgctc cgatcgtcat
     5221 gacctcggac aggtcggtcg ccacgaacgc gcccggcgag gtgccgagca cgccgccggc
     5281 gtgcaggatc ttggactcgg cgtacgttcc gttcgcgtcc ggcgtgaacg gcagcatcac
     5341 cgaaacggcg cccgacagcg cgccgtaggc gccgacgccg cggtcggtcc aggagttcgt
     5401 ggccgggtcg taggacttca ccacgttcca cagcgcctcg tcgaccgcct ggccgaacgg
     5461 accccacatc tgaccgacgc cgtcatagag cacggtgccg tcgtggatga cgtgcaggcg
     5521 cgggaacagc ggcagcgagg tctccccgct cgcgccgttc tcggtccact cgccctgcgc
     5581 cggggcgtag gtgccgtccg cgtcgtaggg gtcgaagatc tcggtttggt ggacgttgag
     5641 gccgctgtcg ttgtagatga gctgcttgac gccgccggcg acgaacaggc ggccgtcggg
     5701 aagggtcacg accgccgggt accagcgtcc gtagttcatg cgctccgccg gcgtccaggt
     5761 gccgccgttg ccgtcggagg agaagatgcg cgtggcattg ttgccgtgca gctctgtggt
     5821 gccttgcggc tcggtgatgg cgccgttgct cagcttgacc agatccaccg gacccggctt
     5881 ccagaccgtg ccgccgatcg cggcgacggt gccgtcgagc aggaggcgct ggtcggcgca
     5941 gaacaggtcg ccctggtcgt cggggtcgcc ggggtcctcg ccggcgtcct cgggcgtggg
     6001 cacctggaag atcggggcgc cgttggcgtc gaaggccagc tggcgggagc ggctgccggg
     6061 gctgatccgg ttcgcgtccg tggcgagcgg atagcggtcg gactcgaggt tctccaggcc
     6121 gttccagtag acgagcttgc cgttgggcat cgccgccagg ctcaccgcgg tcggatacct
     6181 ggtggactcg ctcacgttcg aaggcggagc ggcatcgaaa tggccgttct cgctgaacgc
     6241 gggcaggaac tgacccatcg tcgaagggct tgcggcggcg gccggaacgc tcccacctgc
     6301 gatgagcgtc cccagcagcg ccaggactgc tgccaggggc accagtgcgc gtctgcgatg
     6361 cattgatcct cctcccccgg gcatggcccg gccgatcggt cccggggccg gacccgggga
     6421 ccttccgttt tccttcttcg cggggacagg cccatttcct ctcagccgcc gcatttcgtc
     6481 tcatatggga gcgaaaaagg tcgccgggcg agcccgtccg ggccggaccc gatcccccct
     6541 tcggactatg cgccgggacg gcagtcccac cgaaagatcg gaagggccga aagaaaagga
     6601 acaccgaacc atcgggggcc gacacccgct tggcgggcga ccgagaggaa ggaacaccgg
     6661 atcatggctg cttcgtttgc tgcatcccgc cggatgccgc tcgccgcgac ggcgatcgtg
     6721 ctcggcatcg cgctgctgac ggctcgggcc ggctcggcgg gcaccgtctg cgagccggga
     6781 ggcggcggga ctcctgttcc gtcggcatcg ccggccccga cccccacgat cccgcttggc
     6841 gtgctcggag ggacagccgc ttcgcgagag caggcggcct cgaccgggac ctgcgacgcg
     6901 gcggtcggtt tggacgtcgc accggcgggc ggcggcgtct ggggcaccac cttcgggctg
     6961 tccggatcgc tgtcgtgcaa cggaaacctg ttgggaggca agcaggtcac cttgcagaag
     7021 ctctccggcg gatcgtggac ggccgtttcg agcggcccga cggacgactc cggctacaca
     7081 ttcacgcaga agccctcaca cagcggcacc taccgggtcg cgttcgcggg cgacgagctg
     7141 tgcagcccgg ccgagtcacc cgccaagacg gtgatcgtgc ggccgggggt ggcgttcaac
     7201 gccgcgtcgg gagccacgag ccggcgcggc ggcacggcga tcttcagcgg ctcggtgctg
     7261 ccggctcacc ccgggcacgt ggtgacgctg caggtgctgc agagcggggc gtggcgcaac
     7321 gcggtgacgg tcaagctgga ctcgcagtcg cgctatcgcc tcgcctacac gcgcgcatcg
     7381 ggatcggggc cgctgctgtt ccgggtcgcc taccccacgc agcatgcgga tcacggctgg
     7441 aacgtcagcc ggagcatcag ggtcacctgg agctagcccc aagggcgatt ccggccttga
     7501 acgatgaagg aggccaggcg cgcaacgaac gcacgcacca cgcctagggg accagagcac
     7561 tcgtcaagtt tcttgacgcc gtcaaatttc ttgacgagtc gattgccggc cctttgtccc
     7621 actcctacac ccggcgaaca tgggctacgg ggccgtgctg ctagagcggc gcgaccttga
     7681 tgccggcggc ctccaaggcc gcccgcacgc tcaccgcgtt gcgcaccgcg cccggcgtgt
     7741 cgccgtgcag gcagacggtg tcggcctcga ctaccaccga tccgtcgccg gccggcaccg
     7801 gctccccgcg cgccagcgcg accgcctgcg ccgccgcccg caccggatcc tggatcagcg
     7861 cgcccggctc gtcccgcggc agcagggtgc cgtcggcccg gtagccgcga tcggcgaacg
     7921 cctcgcccgc gacttcgagg cccgccgcgc gtcccgcggc gagcatctca ctgccggcca
     7981 gacccacgag cgtgagcgcc ggatcgaacg ccgcgaccgc ggacgcgatc gcccgcgcga
     8041 tcgccgcgtc ggcggcggcc tggttgtaga gcgcgccgtg cggcttgacg tgccggatgc
     8101 gcagccccgc ccgcgacacg atccggtcga gcgcggcgat ctgctcgacc atcaggcgcg
     8161 cgatctcctg cggcgggatc gcgagcgcgc ggcggcccat cgagtcacga tccggatagc
     8221 cgggatgcgc gccgatcgcc acgccgtgct cgcgggcccg cgcgacggca tcccgcatcg
     8281 tctcgtcgtc gcccgcgtgc gcgccgcagg cgatgttcac cgacgtcacg atcgaataca
     8341 gcgccgcgtc gtcggcgcct tcggcgacgt cggcgttgag gtcgatcgtc ggttcacgca
     8401 tcggtccggc ccgcttgctc cgccagcgcg gcccgcgcag cggcgggatc gcacaggcgg
     8461 aacctcagcc tatcgcccgg ccgcgcctgc gcgagcaccc acagatcggc acggatcacc
     8521 gtcgcgatct tcgggtagcc gcccgtcgcg ggcccgtcgg gcccgagcac gatcggccgg
     8581 ccgtcgcccg gcacctggat cacgcccggc atcgccggct ccggatcgat ctcgggcccg
     8641 ccggggccgt gcgcgagcgc cggaccgtcc agccgcaccc cgacgcgatc cgcgtccggc
     8701 gtcaccgtcc actcctcttc gatgagccgc tcgagcgagt cctcggccag atcgctgggc
     8761 ccggccacga cgcggatcgc atccggggtc gcatccgggg tctcgggccg gttcggtcgc
     8821 ggccgctcgg gcccgccgcg gctcgcggtc ccggcgggca gacggtcgcc gccggcgagc
     8881 atcagtccga taccggcccg gggcgaggcg gagcggcttg cgagcaccgg ctcggtcgcg
     8941 atcccaccgg ccacgcacag gtagccgcgc gcaccgcgcg ccgccttgcc gatcgacagc
     9001 gtcgcgcccg ccgccacggg gacggcgatc tcgccttcga cggcagcgcc gtcgagcgtg
     9061 gcggcgaacg gtgccccggt gatcgcgacg agcgcggcct tttcgaagac gacctcaggt
     9121 ccggcgagca cgaactcgat gccggccgca tccggctcgt tgccgcacaa ccggttgccg
     9181 cggcgcagcg cccactcgtc gaaggcgccg ccgcgcggca cgcccgacgc cccgggcgcg
     9241 gcccggccga ggtcttgcac cgaggagaac ccggcgcgcg tgaggcgcat catgcctcgg
     9301 cgaagcgcac gacatcaccg ggggcgagca tcgccggcgg cacggcggac gcgtcgaaca
     9361 gcgaggcgtc ggtgcggccg agcagccgcc acccgcccgg cgaggccgac gggtacacgc
     9421 ccgcccacac gccggcgatc gcgaccgcgc cggcgggaac gcgcgtgcgc ggcgtggcca
     9481 ggcgcgcagc gtacagcggc tcgggcaagc cgagcagata cgcgaagccg ggcatgaacc
     9541 cgaggaacgc aaccgtatag gtcgcgccgg tatgcagcgc gacgatctcg cgcggcgcga
     9601 gcccgcgctc ggcgccgagc gcgacaagat cctcgccgtc gtagacgacc tcgatgcgat
     9661 gctcgcggat ctcgccggcc gcgaccggcg gcgcggcccg cagcgccgcg tcgacggccg
     9721 cgggatcggc gccgtcgtgc aggtgcgcca acaccgttcg cgcgccgggc accgccgcga
     9781 gcacgtcctc gcgcgtggcg gcggcggccg cggcggcgcg ggcacgctcg ttcgcgcgga
     9841 tcaacggacc gtcgaagcgg acgacgatcg cggactcacc ctgccagcgc cacacgggta
     9901 cagtttgacc gatgcccgag cttcccgaga tgcgcgcgct cgccgagcgg ctcgaggccg
     9961 tggtcggcgg cgcggcgctg gcgcgcatcg agccgatcca cttctcggcg ctcaagaccg
    10021 tcaccccgga cccgtcctcg ctcgccggtg cgcccgtcga aggcatcggc agccgtggga
    10081 agtacctgct gatgcgcgtt cccggcgcgc gcatcgcgat ccacctgtcg caaggcggac
    10141 gcgtggatgt ggaggatccg ccgaagagca cccggcccaa aggcggcgtg gtgcgcttcc
    10201 ggttcgagcg cgccccgtcg attctgatca aagagttcgg caccgaacgc aaggcgggat
    10261 ggtgggtgct cgccgccggc gacgacggcc ccatggcctc gctcgggccc gagccgttca
    10321 ccgacgagat gcggtcgctg atcgtgggcg gcgaggacaa gcggcggctg catacgatgc
    10381 tgcgcgacca acggacgatc gccggaatcg gccgcggcta cgccgacgac atcctgcacc
    10441 gcgcgatgct ctcgccgttc gcgtcgctgt cgtccctgga cgaggccaag cgaacgacgc
    10501 tgctcgacgc ggtccacgag gtgctggagc aagggctcgc cgcggagcgg cgccgaaccg
    10561 gcgggctgcc gcccaagctc ggcggccatt ggatcgttca cggacgcttc ggcgagccct
    10621 gcccgcgatg cggcgagcgg atcgagcgcg tctcctacga gtcgcacgag gtcgcgtact
    10681 gcccggcgtg ccagacgggc ggcaaggtgc tcgccgaccg gcgcatgtcc cgcctgctca
    10741 agtagctatg cccgcgggtt tcccttgaac gcgttggagt ccttgcacgc cttctgcatg
    10801 cgcgcgaccg ccatcgcgta gcgctcgacc tcctcggggt cgaagccgtc ggcctgcacc
    10861 tgcggcgaca gcgcggcttt gagcgcctcg ccggccgtgc gaacctgctc gtgcttcgct
    10921 tcggcgacgg cctgctcggc gacgttgacc cagccttgca cgctgccgag cgtgtcggcg
    10981 tcgggctcct tgacaccgat ggtgttgaag caggtgcgaa cggtcccctg atcgggagcc
    11041 gcgtccgcgt tcgagcaggc ggcgagcgcg agcagcgccg tcacggcaac gcgcctcacg
    11101 gctgctcccc gaaccgcggc ggccgcttct cgcgcacggc cgcgatgcct tcatgcacgt
    11161 cgggtccggc aaagcccatg aactccagcg cgagggatgc gtcgaaggcg ggaccggcgc
    11221 tgcgcagcca gttgttgagc gccagcttgg tccagcgcag cgcggtctgc gagcccgccg
    11281 cgagccggcg cgcgaccgac atcgcggtgt gctcgagctc gtcgtcgggc acgcacatcg
    11341 acaccaggcc gatgcgctcg gcctcctcgc ccgacagcgg ctcgcaggtg agcaggtagt
    11401 acttggcctt ggccatgccg gccagcagcg gccacacgat cgcgctgtgg tcgcccgcgg
    11461 cgacgcccag tttggtgtgc ccgtcgatga tgcgcgcggt cgttgacgcg atcgacacgt
    11521 cggcgagcag tcccaccacc agcccggcgc cgacggcggg tccgcgcatc gcggacacaa
    11581 tcagcttcga gcagttgatc gtgttgtaga ccaggtcgcg cgcttccttg agcacgcgcg
    11641 cgcggacctc gaagtcctgc gccatctcct tgaccagatc caggtcgccg ccggcgctga
    11701 acgccttgcc ttcaccggtg acgattacca cgcgcgtgtc cggatcgcgg tcgatctcga
    11761 gccagacgcc ggcgagctcg cggtgcatat cggcgctcgc ggcgttgagc ttgccgggcg
    11821 cgctcatcac taaacgcagg atgtgcggct cggcctgctc gatcctcagg ctcgaatagg
    11881 cggcgtagcg gtcggtcatc gcgagcgcag gatacgtcag ccgcgggtcg cgccgccgcg
    11941 acgcgtccac gcaccggccg cgagcgcgag cccgccgagc gcgatcacga acggagcggt
    12001 cgcaccgaca ccggtcgccg gcagcggcgc ccgcttcttg gccggcagca ccgtggccgg
    12061 cgcgggcttg tgcgtgcgcg accacgcgag cgcgttctca aagacctggt atccggtgta
    12121 ggtcagcgcg tacgacgaca agccgaacgg gcggtagtgc tgctcgctcg cgtcggggag
    12181 catcgcgccg gcgatgcgca ccacgccctt gccgagcggc aagtcgccga gcgacacgcc
    12241 cgcccgccac ggcgtctggt cgcttgcagg atccggctgg gtgtgcacga acgtcctggc
    12301 tgcgaccgag ccgccggcct cgcgccacgt ctcctcgtcc acgatccagt tcggcgagtc
    12361 gcaccggtcg atctggcagt tgtcgttcga cgatccgcga ggcgagatgt agtagccgag
    12421 cggtcccggc tcgcaggtct gatgccggtt gtcgtattcg acgccgccga gcgtgtcgaa
    12481 gccctcggac gcgccgggct tgttgacgtc cttggcgagc ggatgccgct cataggtcgg
    12541 cccgtcgccg tcgtcgaagt ccatgtatcc ggcgtagaag tagccgcgtc ccaccttcgc
    12601 gggcgcgatc tgcgggttca gcagcggcaa gcccgacaat gccgagtcgg tcaaaacgag
    12661 cgcgcctccg ccggcggcga aggcccgcaa cgcggacgag tagcgctggc ggccggcgcc
    12721 gtcgaagcgc gagccgggcg cggatccaac cgagtcgaag ccgtgcagca tgccgggcgt
    12781 cgccagaatc tgctccaccg tcaccggctc gagcttcttg ccggcgggaa cgtagcggtt
    12841 gagatccttg aagaagtcca tgcgggatcc gtcgtagggc atctgcccgc ccggtgtggc
    12901 ggccccgggc gtgaaccgga ccgtgacctt cgacggcccg atgcggccgt cgtccggccg
    12961 gatccggtac gggcccggct tcgggttgtt gatgtccaca cgcgcgcccg cctgggcgta
    13021 ggtggccgac tggttccagt agcggcccgc ctcgcggcag ccttcgggat cgtcgaaatg
    13081 atcgggcggt ccgcagtagt cgatgatgaa gccctcggtg agcagcccgt cggccgacat
    13141 gctccgcacg ttcgagaacg tggcctcgac ggtcatgccg ccgttgaaga tgccgtcgga
    13201 cggacccttg acgtcgaagg tcacctcgtc cgagccgttc ggcatgtcga gggatatcgg
    13261 cggctgcggc ttcagattcg ccagcgggga ggcgaccgcg gccttgcccg cattgcggat
    13321 gcgcgccggg tcgaacacgt agccgacctt gcccttcggc gcgtagcgga ccggcttctc
    13381 atcgagcagc gacgcgatct gcgaatagat caagcccttg ttgccgtcca cgtgaagctg
    13441 ctcgatcccc gggtcgaagg ccgtgttcgg agccaggtgc gagtacgcca tctcgttgct
    13501 cacgccgacc gcgtccaggc cgagcggcga gtccatccag tcaccgaacg agccggtgac
    13561 ctgataccgg atcatgtccc agaccgtgcc ccacaggtcg gtgcacatcg ggccgtgttg
    13621 cccgccgaag aacggttcgg gcagcgggcc ggggcacgag tcgttgcgcg cgaccagcgg
    13681 cgaccaggtc aggcgctcct ccgcgtcgcg ccaggtcgtg atcgcggtct gcaccgtgat
    13741 cgcgttctta cggtaatcgc gctgggctga gccgagcatc gtgaacgaga acgcatggct
    13801 ggtcaccatg ccgtgcaggt cgatcgagcc ggcaaacgcg cccgccgagg tcttggcgcg
    13861 catcttgcga aggaacttcg cgtagccgcg cgtctcgggt tcgctgccgg gcgtgtactc
    13921 cggctggctg taaccctgcg gcggccagtc acggttcacg tcaatgccgt tgccgttgta
    13981 gcgctggaag aagacgtttc ccttggtgac ctctccgcgc gaccacccgt cggggttcga
    14041 cagcacgaag tagatcacgg ccttgttcag cacctcaccg gccgtgggtc cttccggcgg
    14101 atcgaacgcg tcgagcagcc gcttggggcg ctcgggcgag acgccttcgt tcgcgcaggc
    14161 gggcgaggca tccgcggact tctcgcatgc ggcccaggtc accagatcct cggccgcgcg
    14221 gatgcctccc tcgatgccgg cacgctcgat gccgtggatc gacagggagt aggcgaaatg
    14281 cttgcggtcc ttctcgggca ccttggacga ggcgtcggtc accttgatca cgtgcaggtc
    14341 ccgcttggcg cggctcagat cctcctgcgg aatccctgcg gactgcagct cgtcttcgag
    14401 gaagtcggga tcggtgacga acttctcgcg aaggttgatc agctcgatca tgccctcgaa
    14461 gcgggactcc agatagccga gcccgtcgag cacgtcctgc cactgcagga actgcacggc
    14521 acagacgttg cccttcgccc acggcctcgc gccgggcagg ccgcgcaggc agccgtgcgg
    14581 atccgggaac acgcggccgt acgcgagata gcgggcgtcg tcggccgcga ccggaaaggc
    14641 gttcgcggtc ggaagcgggg cgagcacgcc ggccatggcg accacgatca aggatgcggc
    14701 ggcggccgcc aaccgtttag gatcgctccc ctttcgacgt ggcgggccgg gctccctgcc
    14761 ggagcgcggc catcacggaa gatacgagat attctcccgc tgcgtgaagt tcgtcctgcg
    14821 tccgcgcgtg cgcaccgcgg cccgaatcgt cctgctgcag cgcttgctgt tcgccggggc
    14881 aggcttgctg atcgcctggt tcctcgcgcc cggcctcggc cggctcaccg acggcgtgct
    14941 cgacgtgtgg gatcgtctgg acgccagccg cttcgcgcgc atcgccgagc acggctacga
    15001 caaccccgac gacccccacg ccgcggcgta cttcccgctg gtgccgatgg tgatggccgc
    15061 cgcgcgcgcg atgcggatcg agcccatcgc cggcgcgatg atcgtcaaca ccgtggccgc
    15121 cctggtcgcc ttcgtttatc tgtaccgctt ggtcgaggaa gagttcgacg ccgagaccgc
    15181 gcgccgcgcg ctgtggtacc tggcgttctt cccgaccgcg gtgttcctgg tggcgcctta
    15241 ctcggagccg ctgttcctgg ccggcgccat cccggcgttc tactacgcgc gccggcagcg
    15301 atggcatctg gtcgggctgc cggccgcgat cgcctgcacg gcccgattcg ccggcgtgtt
    15361 ccttttgatc ggcctcatcg ccgagttcct tcgccagcga agctggcgtc cgcggcgcgt
    15421 ggcgaccgcg gcggtcgcgc tggcgaccgg cgcgcttccg atcgccgtat tcttcctgta
    15481 cctatggcgg gtgaccggca gcccggcccg cttcttcgag gcgcagcgcg acgggtgggg
    15541 gcgaatcttc gtcggcccgg tccggtcgtt cggcacgacc tgggaaacct ggaccggctc
    15601 gcacttcttc accaaccagc tcatcgcctg gcgcgtcgag attgtcgccg ccgcgctggg
    15661 cgtcgcgctg gtggtgtggg ccgccctcaa gcgcgagtgg ggctatgccg cctacatggg
    15721 ctcgatgcac gcggccttgg tcaccagttc ctggtactac agcatcccgc gtgcgatgct
    15781 cacgatgttt cccgcagtga tcttcctggc ggcacacacc gtgcgaaagc cgaagcgtca
    15841 cgagccgctg ctcgcggtgt tcgcatcgat cgcaatgttc ggcgtgatcg tctacacgcg
    15901 cggccactgg ttcttctaga cgaccaagac cgcgatcggc ttcgggccgg gggattcggc
    15961 gccgccggcg cggtcggtcg cgacgacgcg gacctcgtag cggccgcggc ccgcgggcgc
    16021 ggccgccgcc cacgtgcagg agcgcgcgct cgcgtcctcg cacgaaattg tcgcgaccac
    16081 ggtgacgccc ggcgccccga ccggcacgaa ggtcaccgcg acctcgcgca gaccggatcg
    16141 gtcgtcggtg acggtgccgc gaacgaaacg ctccatcgcc ggcggcagat gcgcgatcac
    16201 ggcctgatcg ggcgtgacga tgacgctgcg cggcggcgcc gtatcgatcg tccattcgag
    16261 ccgggccggc gtcgggtcgg cgagccccga cgcgtccacg gcgcggacct cgaacacgtg
    16321 ctcgccgtcg gcgaggccgg tcaggttcac cggcgacgtg cagcgcgaga acgcggcccc
    16381 gccgaggctg cactcgtaca tcagccagtg cggcggcgac acagagtccg agccggagaa
    16441 cgtgaagcgc gcgtcgcgcg aggcggtaaa cgcggcggga cccgcgccga gcgtggtctc
    16501 gggcggggtg cggtccacga accaggtgcg gctcgccggc gtcgcatccc ggtttccggc
    16561 gccgtccacc gcgcgtaccg agaacacgaa catgccctcg tgcgcgaacg tatagctcac
    16621 cgggctggcg cacgcgctga aggcggccgc gcccaggcgg cactcgaacc acagcggccc
    16681 gggcgcgtcg tcggtcgcgc tgaatgcgaa cgtggcacgc tcttcggtga cgggcccgga
    16741 cggcccggac gtgatcacgg tctccggagc gaccgagtcg gcgaaccagt cgtgggtcgc
    16801 ggcgagcgag acgttgcccg cgagatcccg cgcgcgcacg cggaaggacc ggctgccggt
    16861 ggcgatcgcc gggtaggcct tcggcgagac gcacgtggcg tagggagcgc cgtcgaggct
    16921 gcactcgtgg acgaggacgc cggccgcggt gcgatcgtcg gcgccgtcga aggcgaagac
    16981 cgccgagccg atcccgcccg gatccggcgg ggccgacagc aggcggaccg acggcgcgac
    17041 cgtgtccacg atccatgacc gtgatgccgg aatcggatcg gcatgaccgg ccgcatcgag
    17101 cgcgcgcacg cgcaccacgt gcggcccgtc cccgaggccg gtccagcttc gcgggctcac
    17161 gcacggagcc cacggctcgc cgtcgaggct gcattcgaac gccgccgcag acggcgaccc
    17221 tgcgaaggcc agtgtggcgg cctgctcgga cgtccacgcg gacggcccgc ccgtgatcgc
    17281 ggtgtcgggc cgcgcgacca cgaagtcgat catgcgcccg gcgctccccg catgatacgg
    17341 ccaagagcgg ccgccggcca gcgccgcgtc gcgcatgcgc gtgaaagcgg cgagcgctcg
    17401 cgactgcatg cccgcttcgg tgaacgcgtc cgcgaacgac ggccaccagc cgatcgcacg
    17461 aacctgcggc ccttccggcg ggttgaacag ccccggcgac cacgggtcgt cccagctcgg
    17521 gttctcgcgg tcgatgcgcc cggcgagcga tgccgcgcgg gcggccggga ccagaccggt
    17581 gttgatcatc cagacctggg cggtcaccga cgggtagaac tcgctccagt ccgccggatg
    17641 ccgcaccccg ccggatccga gcgcgacgtc gtaggcgccg gccgcctcgt tccagaagct
    17701 cgcgagcgag acccgcattc cgccgatcca ttcgtcggcg cgcgcgcgca gcgccgcgtc
    17761 gccgtgcacg agcgcgagct cggccaccgc gatccatccc tcgtagacct ccgcgttgtc
    17821 catcgcgtac atcaggttcc agccgggctt ggcgtacgtc aggtagccgg gcgccgcggt
    17881 tgcgagcatc gcgtcggtgg cgcggcgcac cgcgggccag atcgccgcga gccgctgcgc
    17941 gtcaccggtg gctgcgtatg ccgcgcgcac cgccgacagg aaggtcgccg cgtacgcgtc
    18001 ggtcgagtcg tagtccccgg tcgaaaccca gctcccgccg ctcagctcgt agtcggtgac
    18061 gaagccgctc gcgtccatgt gggcggcgta ccagtcgagc cagcgccacg ccgcctcggc
    18121 atagcgagca tcccccgagg acagcgtcgc cgacgcgagc cccgaggccg ccatgttggc
    18181 gaagtacggg atgcacaagc tgcggggctc gttcatcgcg atcagcccgg cgtgcgggcc
    18241 cgagggcacc tgaagcgtca ggatccactc cgcgtcctcg acgaccggat cccccgcggc
    18301 ggcgcgcacg ccgcttcccg gcatcatcgc ggcgagcgcg agcagcacgg ccatgccgcg
    18361 cctgagcgcg gcggacgacg gggagggcct tttcatcgtg cacggacaag ttcgcctcgc
    18421 acctcgccgc gtcccgtcgg cgcggtcggc acaaagcggg gaaaccggta gctgcggccg
    18481 actcgctatg cttcgcgagg tgaccgagca accgaaggat ccgacgcatc cgcacttcgg
    18541 cgaggagggg cgccgcctgt cctacggcac ctatctgaag gtccccgagc tcatcgacct
    18601 gcagcttccg ctcgcccgtc ccgaagccca cgacgagctt ctgttcatcg tggtgcatca
    18661 ggtctacgag ctgtggttcc gccagctgct gttcgagctc gacgcgatcc gcgagcatct
    18721 gttcgccggg cgggcgccgc aggcgcgcca tctgctcgag cgcgtccacg agatcgagcg
    18781 ggtcctggtc gcgcaggtcg ctgtcctgga gacgatgacg ccctcggact tcatggactt
    18841 tcgtcgcaat ctggcgcccg cgagcggatt ccagtccgtg cagttccgcg agatcgagtt
    18901 cgtgtcgggc ctgaaagacg agggcgtgat cgcgcgtttg gatccgcagg ccggcgaggc
    18961 cgcccgcttg aagcgccggc tcggcgagcc gacgctgtgg gacgcttact gcgaactgct
    19021 cacctcgcgc gggctgccga tgccggacga cgacgcggac cggcggcgcg actcgctgct
    19081 tcgcatgacc cgcgaacgcg accgattcgt ggacgagcac gcgatcggcg aggcgctgct
    19141 cactcacgac gagctgttcg cgctgtggcg tcaccggcac gtgctcatgg tcgaacggca
    19201 gatcggcacc aaaagcggca cgggcggatc ggccggcgcc ccgtacctgc ggagcacgct
    19261 cacgcggcgc ttctaccccg aattgtggga cctgcgcagc tacctgtagc ggcaggccgc
    19321 acggatgaat gggccgccga acagaccggc tggatccgtt tgccgccgag ctggcgctgc
    19381 gcaaccacgc gtgatgccgg attcgtccgc ataacggacg gtcttgcgac gtcggcgaac
    19441 tcctgtctca agacacgacg gacggggtac gatgcgaaga ccagatgatt atcgacggct
    19501 tcgcgcatca ggttcccgac atcgatcccg gcgagaccga ggaatggctc gccgcgttcg
    19561 acggagcgct tgacgcgcgc gggaaggccc gcgcgcggta tctgctcgtc aagctgcttg
    19621 agcgcgcgcg tgagcgcggc gtcggcttcc ccgcgaccgt ctccacgcac tacctcaaca
    19681 ccattcccgc gcatctcgag ccggagttcc ccggcgacga gcacatggag cgccgcatcc
    19741 gtgccttcat tcgctggaac gcggccatca tggttgttcg cgcgaacctg atcaacgccg
    19801 gcatcggcgg gcatctcgcc acctacgcat ccagcgccgc actctacgag gtcggcttca
    19861 accacttctt ccgcggcaag gatggcggca cctacggcga tcaggtcttc atccaaggtc
    19921 acgcatcgcc cggcatctat gcgcgcgcgt tcctggaagg acgcctcacc gaggagcagc
    19981 tcgaccactt ccgtcgcgag gtgaacggcg gcggcctgtc gtcctacccg cacccgcggc
    20041 tgatgcccga cttctgggag ttcccgacgg tgtcgatggg gctggggccg ctctcggcga
    20101 tctatcaggc gcgcttcaac cgctatctgc tcaaccgcgg gctcgtggac acgagcgctt
    20161 cgaaagtgtg gtgcttcgtt ggcgacggcg agaccgacga gcccgagacg ctcggcgcgc
    20221 tttcgatcgc ggcgcgcgag aagctcgaca acctgatctt cgtcgtcaac tgcaacctgc
    20281 aacggctcga cggcccggtg cgcggcaacg gcaagatcat ccaagagctc gaggcggtct
    20341 ttcgcggcgc aggctggaac gtcatcaagg tcatctgggg ccgcaagtgg gacgagctgc
    20401 tggccaagga ccatgacggc gtgctcgtcg ccaagatgga ctcgaccgtg gacggcgagt
    20461 tccagcgcta cgcgaccgag accggcgcct acatccgcga gcacttcttc ggtccggacc
    20521 cgcggctgcg cgccatggtc gagcacctgt cggacgagga cctgcgcctg cttccgcgtg
    20581 gcggccacga ctatcgcaag ctgttcgcgg cctacaagca tgcggtcgag cacgaaggaa
    20641 cgccgacggt tatcctcgcc aagacgatca aaggctggac gcttggcccg gacgtcgaag
    20701 gccgcaacgt cacgcatcag gtcaagaagc tcacgcaaga gcagctgttc gagctgcgcg
    20761 agcggctgtt cctgcacgag gaggttcccg agtcggcgct gcagcaggag cttccgccct
    20821 actaccgtcc cgagccggaa tccgacgagt acgcgtattt gatggagcgg cgccgtgcac
    20881 tgggcggctc gctgcccaag cgcgtggtgc gacccgagca ggccgagacg ccggccccca
    20941 agggcgaggt cttcgacgac ctgaccacgg gaagcggcac gcaagaggcc tcgaccacga
    21001 tggcgttcgg ccggctgctg cgcgacatgc tccgggacaa gggcttcggc cgcttcgtcg
    21061 tgccgatcat ccccgacgag gcccgcacgt tcgggctcga cgcgctgttc cgccaggtcg
    21121 gcatctacgc accgcacgga cagctctacg agccggtgga cgcgcatctg ctgctgtcct
    21181 acaccgagtc caagcagggt cagatcctcg aagagggcat caccgaggcg ggctcgatgg
    21241 cctcgttcat cgcggccggc acgtcctacg cgacgtgggg tgagccgatg atccccttct
    21301 tcatcttcta ttccatgttc ggcttccagc gcatcggcga tctggtctgg caggcgggcg
    21361 atgctcgcgc acgcggcttc atgctcggcg cgacggcggg tcgcaccacg ctcaacggcg
    21421 agggcctgca gcacgaggac gggcactcct tggtgctcgc ctccaccgtg cccaacgtca
    21481 tggcctacga cccggcgttc gcctacgagg tcgccacgat catccgcaac ggcatcgacc
    21541 ggatgaccgg taaggacgcc gaagacctca tctattacct cacgctctac aacgagaact
    21601 atccgcagcc gccgatgccc gagggtgcgg ccgagggcgt gctgcgcggc ctctacaagt
    21661 tcgcggccgc gccgaacggc ggcaagcgac gcgcgacgat cctgttctcc ggaaccgcat
    21721 ggaaggcggc gatggaggcg caacggatcc tgtcaaagga ttgggacgtc gccgccgact
    21781 gctacagcgc cacctcgtac aagtgcctgc gcgagaacgc gctgggcgcg gagcgctgga
    21841 accgtctgca cccgaccgag acacctcgcg ttccctacgt cacacaggtg ctcgccgagg
    21901 ccgcgagccc gaccgtggcg gtgagcgact tcatgaaggt cgtgcccgac cagatcgcac
    21961 ggtgggcgcc gcagccgttc acgccgctcg gcaccgacgg ctacggacgc tcggacacgc
    22021 gcgagacgct gcgccgccac ttcgagacgg acgcggcgca cgtcgtcgtc gccacgctgc
    22081 acaccttggc gcaggccggc gacgcgaagc cgcaggaagt catggacgcg atcgggcgct
    22141 acgacatcga tcccgacgcg atcgaccccc tgcacgccta gcaccaccca cccgtttccg
    22201 ggcggcccct gcgggtccgg gacgcgccgc ccgggattcg ccgagtacgt tcggacgacc
    22261 cgcgcattct tccccatgcc tcacgaacgc ggggcctgat atcctttgcg cgatccgaag
    22321 gggagtatcc tcacggcact gcccgtcatc acggagctcg caccacgcga gcgcccgggt
    22381 ttgccggccc gtcagggtgg agagaccttc ggctgaacag ccaaacgttc gagccgaagg
    22441 agcgagcagc acatgcacgt cgagccctgg atgtggatcg cctttactgc gttcatcctc
    22501 gcgatgctcg cgatcgacct attgatgaac cgggacgcgc acgtcgtgtc tatgcgcgaa
    22561 gcaggcatct ggagcggcgt gtgggtcacg ctgggcttgt tgttcgcagg gcttctgtgg
    22621 atctggcagg acggaaaggt cgcgggcgag tacctggccg ggtacgtgat cgaaaagagc
    22681 ctgtcggtcg acaacatctt cgtcttcgcg ttgctgttct cgtatttctc cgttccggcc
    22741 aaataccagc atcgcgtcct gttctggggc gtgctcggcg ctctggtctt ccgcatcctg
    22801 ttcatcgccg gcggcgccgc gctgctcgac aacttccact ggatgatcta cgtcttcggc
    22861 ggcttcctgg tgatcacggg catccggatg gcgatgcaca aggaaacgga ggtgcatccc
    22921 gagaagaacc cggtcttgaa gctgatgcgc cgcgtgatcc cgatctcggg ctcttacgac
    22981 ggcaagcact tcttcacccg cgtcaacggc aagcggctcg ccacgccgct gttcgccgtg
    23041 cttgtggtgg tcgagaccac cgacatcatc ttcgcggtcg actcgatccc ggccatcttc
    23101 gcgatcacgc gcgacacctt tatcgtcttc acgtcgaacg cgttcgcgat cctgggactg
    23161 cgggcgatgt acttcctgct cgccggcatg atccgcaagt tccagtatct gagcgtcggg
    23221 ctatcggcgg tcctggtctt cgtcggcatc aagatgctga tcagcgacgt gtatcacgtg
    23281 ccgatctggg cctcgctgtc gttcatcgcc gtggcgatca ccgcgtcggt gatctactcg
    23341 ctcaggcatc cggccgcgcc ggccgagatc ccgcatggtg aaggcctgca ggagctgcgc
    23401 gaggacgctc aggagatgcc gcggtagcgt cgcaccgacg ccgggacgaa taccagcagc
    23461 agcgccgcga tccacgcgag cgatttgacc gccggcacca gcgccggacc ggcgacgagc
    23521 agcccgcgca tcgcgtccac ggtgacgctg accgggttca cgctcgccca cgcctggagc
    23581 cagcccggca tcgaggccac cggcacgaac gcgctcgagg cgaacaccag cgggaagacc
    23641 cacacgaacg aggcggcctg cgtggactcg acgtcgcgca cgctcagccc gatgaatgcc
    23701 gagatccacg agaacgcgtg cccgaatagc accgcgagca ggatcgcggc cacggccggc
    23761 gcgacccccg tgtggatccg gaagccgacc agcgttccga tcagcagcag cagcatgacc
    23821 acgaacaggt tccggaccgc gtccgagagc gtgcggccgg cgagcaccgc gctgcgcgcc
    23881 atcggcaggg accggaagcg gtcgatcatg ccgtaggtca ggtcttgggc cagcgcgacg
    23941 ccggtcgtgg tcgagccgaa cacgacgacc tggccgaaga cgccgggcat caggtagtcc
    24001 acgtacgcga tgccgtcgcc gggatcgatc gcgccgccga acacgtagcg gaacagcagc
    24061 gtgaacatca ccggctggat cgtcgagaac accagcagcg tcggcacgcg ctgatagcga
    24121 atcaggttgc gcttggtcac gatccacgcg tcccacagca tcagcagcgc gcggttgcgc
    24181 gtgatggtca tcggctcgct gctcacgcgg tcctcttccg gccacgcccg cgccggccgc
    24241 ccgccgcgac gcgtgcctct tcggcctggg tcgcctcgtg tccggtcagc cgcaggaaca
    24301 cgtcgtcaag cgtcgggcgc cgcaccgcga agtcggcgag ctcgattcgc gagcggccaa
    24361 ggcgctgcac gacctcggtg atgatcttgg gtcccttcgc ggcgatcggc agcgtcacgc
    24421 ggcgccggag cgcgtccacg agcggctcgc ccgagccgag gtcggcgatg cgctttgcgg
    24481 cggcctcgac cttggcggca tcctcgatgt gcagctcgag cacgtcgccg ccgacgcggt
    24541 gcttgagctc gtcgctggtt ccttcggcga tcacgcggcc gtcgtcgatg acggcgatgc
    24601 ggtgcgcgag ccggtcggcc tcgtccaggt actgggtggt gagcagcagc gtcatgcctt
    24661 cgccgacgag ctcgtcgatc atctcccaca gctcgatgcg gctgcgcggg tccaggcccg
    24721 tcgtgggctc gtcgagaaac aggatcttcg ggcggcccgc caggctcgcc gcgaggtcga
    24781 gccggcggcg catgcctccc gagtaggtct tgacgacgcg gtcggcggcg aagctcagcg
    24841 agaagcgctc gagcagctcg cccgcgcggc gctccgcctc ggcgcggcgc aggtgataca
    24901 gccggccgac catgacgatg ttctcgcgtc cggtgagcgt gtcgtccacg gccgcgtact
    24961 ggcccgcaag gccgaagacg tggcggagcc ggtcggcgtc gcgcaccacg tcgtagccgc
    25021 cgaccgtggc gcggcccgcg tcggggacga gcagcgtcgt caggatccgc accgtggtgg
    25081 tcttgccggc gccgttcggc ccgagcaggc ccagcaccga tcccgcggcc acacgcagat
    25141 ccacgccctt gagcgcctcg acctcaccga accgcttcgc gagcccttcc gcgaccaccg
    25201 ccgcttccaa cgcatccctc ccgccggccg aacacgcggc gaagactaac gcaggcgctc
    25261 ccggccgggc gcgagagctt aggccttccc ggccttgacg gtcccttcgg ccttgctgct
    25321 gcgggccgcg acgtcctccc gcggcgcgat gcgccggact gcggccgagg cgcgctccca
    25381 gccggcgagg atctcggcgg cgcgcgcgct gccggtgacg gccatgtgcc gctcgatcag
    25441 cgccttgacc cgcgcgagcg cctcgtcgcc gggcttgcgc acgtccacca gatcgggatt
    25501 gacgcgcgcg ggcagcgacg cgtccgggtc gtagacgaac gcctcgccgc cggtcatgcc
    25561 cgcgccgaca ttcgcgccgg tcgggccgag cacgaccacg gtgccgccgg tcatgtactc
    25621 gcacgcgtga tcgcccgtgc cttcgacgac cgcggtcgcg ccggagttgc gcaccgcgaa
    25681 ccgctcgccg gcgcggccgg cgaccagcag cgtgcctccg gtcgcgccgt acaggacggt
    25741 gttgccggcc aggtacggat cgcccgcgtc gtcgccgggc gccacgatcg cgatcaggcc
    25801 cccgcccata cctttgccga cgtagtcgtt ggcctcgccg tacaggatca gctcgacgcc
    25861 ggcggcgagg aacgcgccga aggactgtcc ggcctggccc gtcagctcga tgcgcgcgcg
    25921 gcccggcggg ccggcggcgc cgaactcgcg tccgatcatg ccgccgagcg tggcgccgac
    25981 ggtgcggtcg ccgttgccga tcgcgtagcg aagctcgatg cgctcgccgg cgcggatcgc
    26041 gtcgaaggcg tccccggcga tgcgatggcc gagcgcgtcg cgcggccgca tcgtgggaac
    26101 cggctcgacg aagcgtcgcg gtgtggcggc ggcggtggcg gcggcgccga tcagcggcgt
    26161 caggtcgagc agcaacgccc gctcgcccgc atcggcgcgc acccgcagca ggtcggtgcg
    26221 tccgatcgcc tcgtcgagcg agcgcagccc gagcacagcc agataccggc gcgtttcctc
    26281 ggcgacgaag cgaaggtagg cggcgaccat ctgcggcgtg cccgcatact tcgcgcgcag
    26341 ctccggccgc tgcgtggcga tgccgaccgg acaggtgtcc agatggcagg tgcgcaccat
    26401 gatgcaccct tcggcgagca gcaccgcggt gccgaacgag tactcatcgg cgcccagcag
    26461 cgccgccacg atcacgtcgc ggccggtctt gaacccgccg tccacccgca gccgcgcgcg
    26521 tgtgcgcagg ccgttctcga cgagcgcctg ctgcgcctcg gccaggccga tctcccaggg
    26581 catgcccgcg ttcttgatcg aggtcagcgg cgaggccccg gtgccgccgt cggcgccgga
    26641 gatatgaacg atgtccgcga ggcctttgat cacacccgcg gcgatcgtgc ccacgcccgc
    26701 ctcggcgacg agcttgaccg acacgtcggc ggccgggttc gcctgcttca ggtcgaagat
    26761 gagctgcgcg agatcctcga tggaatagat gtcgtggtgc ggcggcggcg agatcagcgc
    26821 gacgccgggc tgcgtgtgcc gcagccgtgc gatctcctcg gtcgccttgt ggccgggaag
    26881 ctgtccgccc tcgcccggct tggagccttg cgcgatcttg atctggatct cctcggcgaa
    26941 ggcgagatac tcgggcgtca caccgaaccg gcccgaggca acctgcttga tcgcggagtt
    27001 cttgctcgtg cggtagcgcg ccgggtcctc gccgccctcg ccggtgttgg cgcggccgcc
    27061 gatcaggtgc atcgcctcgg cgagcgtctc gtgcgcctcc ttgctgatcg cgccgtggct
    27121 gatcgcgccg gtcgagaagc gccgcaggat cgcctcggcg ggctcgacct cctccagcgg
    27181 gacggactcg ggcgcggcga ccagatcgag caggtcgcgc ggcgcggcgg gcggacgtgc
    27241 gttgaccatc gcggcgaagc gttcgtagag ctccgcgttc ggctcgccgc gcaccgcggc
    27301 ctgcagggtc ttggccgcga tctcgtcctc gcccggcgcg tcgtcgccgt cctcgcccga
    27361 ttgcgtcgag gtcaggatct cgccggtcgg atcgaccgtg cggtgcagcg cgcgcacgac
    27421 cgacgggctg ttggtgtgct gctcgccttt gctcgagaac ttcacgaagc cggggttggc
    27481 gagcacgggc ttcttgtcgt gggcgccgta cgcggccgcg tgccgctcga gcacgtcggc
    27541 ctggatctcg tcgaagccga tgccacccag cggcgagacc gtgccctcga agcacagctc
    27601 gatcaccccg gcacccacgc cgagcgcgtc gaagatcgcg gcgccgcggt aggagtccac
    27661 ggtcgagatc cccatcttcg acaggacctt gagcacgccc tcctcgatcg cgatcttgaa
    27721 ctggcgcatc gcctcgtcgg gtgagccgac gtcgcgtgcc cgtcccgacg acgccaggct
    27781 cgcaaccgtc gccagcgcaa gcctcggata gatcgcctcg gcgccgtagc ccagcaggca
    27841 cgcgaagtga tgcgactcgc gcgcgtcgtc ggtctgcacc acgatcgagc agcgcgtgcg
    27901 atggccgctg cgcagcagcg cggtgttgac cgcgccggtg gcgagtagcg acgggatcgg
    27961 cgcgcgcgac ggcccggcgg ccgcgtcgct cacgaccagg atgccgctgc cgctggaggc
    28021 ggcgcgcacc gcggccgccg caatccgctc gagcgcgccg cgcagcgcgc ccggcccctc
    28081 gttgggatcg aacgtcgcgt cgatcacggc gccgcccggc ggcttgaaca gcaggaagga
    28141 gtcgtactcc agatgcgcgc acgcctcggg ccgctcccac agcagcggat cctgcgggcc
    28201 gatcagggtg cgcaggctca tgaccgcgcg ctcgcgcaga tggtcgatcg ccgggttggt
    28261 gacctgcgcg aagcgttgct tgaagtagcc gtagagcgtg cggcgatgcg acgcgatcgc
    28321 ggccatcgcc gtgtcgtcgc ccatcgagaa cgtcggctcc ttgccgttgg ccgccaccgg
    28381 acgaaggatc aggctcgtct cctcacgcgt gtagccgtgc gcgacctgcc ggcgcaccag
    28441 gtcctcggga acttcgatca cggcgtcgcc gggggactgc accgtccggt actcgctcag
    28501 ccacgcgccg tagggctgcc ggccggcgat gcgccggatc gggtcggtct ggaacccgcc
    28561 gtcggccgga tcgacgaaga ccatctcgcc gggtccgatc tttccgcgct tgacgcgtcc
    28621 gtggccgctc acgtcgatgg ccccggcctc gctgcagacc gcgacgaagc cgtcctcgca
    28681 cacgtgcgtg cgcaggggcc gcagcccgtt gcggtccagc gccgcgccca cgcgcacgcc
    28741 gtcggtgaag atcagcccgg ccggcccgtc ccacggctcc atcagcgagg cgtgccagcg
    28801 gtagaagtca cggacctccc ggagcatcga cgcgttctgc tcccatgcgg ccggcaccag
    28861 catcgcgacg gcgtggcgga tgtcgcggct ctcgccaggg tattgccctt cgcgggcgag
    28921 aagctcgagc acgttgtcga gcat
//
LOCUS       DATLFO010000322         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_97839, whole genome shotgun sequence.
ACCESSION   DATLFO010000322 DATLFO010000000
VERSION     DATLFO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97839"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VM841_13100"
     CDS             complement(<1..455)
                     /locus_tag="VM841_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVL91163.1"
                     /translation="MRRLLAETRLDASRFVLPLFVQDGLADPASIEALPGHRRWAPEQ
                     AATPAKEAAAAGIPAVLIFGIPDRKDAAASRAYAADGPAQRAVAAIKESAPDLAVFAD
                     TCLCSFTDTGHCGVLRDGEVDNDASVELIARAAAAQAAAGADVVAPSDM"
     gene            complement(488..1234)
                     /locus_tag="VM841_13105"
     CDS             complement(488..1234)
                     /locus_tag="VM841_13105"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HVL91164.1"
                     /translation="MNLSGRSVLVLRPRDQADALAGLLRERGAEPVVVPAIEIQAPEN
                     SVDIDAAIRDATTFEWAVFTSVNGVRIVMDRVHELDVPFDPAHIGAIGPGTARALHGS
                     GYGEVWTPPVYTTDALAEELPDARGEVLLVRAEAAGNELDRRLRDRGMRVRRIDAYRT
                     VETNHDAIRAALHGGVNAVLLTSASIAQSFASAAGSATVPPVCCIGPATAAAARHAGL
                     RVDAEAERHTVDGILHALESLPEQRHGRAS"
     gene            complement(1231..2115)
                     /gene="hemC"
                     /locus_tag="VM841_13110"
     CDS             complement(1231..2115)
                     /gene="hemC"
                     /locus_tag="VM841_13110"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVL91165.1"
                     /translation="MRTLIAGTRGSALARTQTGSVIARLRIAAPGTRIDERLIRTHGD
                     TSISAPAGDGLFVKELQEALRAGRIDIAVHSFKDVPTEPVDGLRVAAVPVRTDPREAL
                     VGSTLAALPAGARVGTSSPRRAAQLLAVRPDVIPTSIRGNVDTRVAKVRGGDYDAALL
                     AAAGLIRLGIEPDEIIDARDMLPAPAQGALAVEIRDDDHEVAALVAAIDDPATRRCAE
                     TERAALRELGGGCMLPVGILAEAGDDRVMRVRGGAVSADGRRVVRIDESVDDDDPVVA
                     GTKIAAALIAAGALEVIA"
BASE COUNT          289 a          805 c          784 g          313 t
ORIGIN      
        1 atcatgtcgc tgggcgcgac gacgtcggcg cccgcggcgg cctgcgcggc ggcggcgcga
       61 gcgatcagct cgacgctggc gtcgttgtcc acctcgccgt cgcgaagcac gccgcagtgg
      121 cccgtatcgg tgaagctgca caggcacgtg tcggcgaaca cggccaggtc gggcgcggat
      181 tccttgatcg cggcgaccgc gcgctgcgcc ggcccgtcag cggcgtacgc gcgtgacgcg
      241 gccgcgtcct tgcggtcggg aatgccgaag atcagcaccg ccgggatgcc ggcggccgcg
      301 gcctctttcg cgggcgttgc cgcctgctcg ggcgcccagc ggcgatgccc gggcaatgcc
      361 tcgatcgacg cgggatcggc gagtccgtcc tgaacgaaca gcggcagcac gaagcggctc
      421 gcatccaggc gcgtctcggc aagcagccgg cgcacggcat cggatccgcg gagccttcgc
      481 agccgggtca cgatgccctc ccgtgccgct gttcgggcag cgactccaac gcgtgcagga
      541 tgccatccac ggtgtgtcgc tcggcctcgg cgtccacgcg aagccccgcg tgccgcgcgg
      601 ccgccgccgt tgccggtccg atgcagcaga ccggcggaac ggtcgccgag cccgccgccg
      661 acgcgaacga ttgcgcgatc gaggcgctgg tcaacagcac cgcgttcacg ccgccgtgca
      721 atgccgcgcg gatcgcgtcg tggttcgtct cgacggtccg gtaggcgtcg atgcgccgga
      781 cgcgcatgcc gcggtctcgc agacgccggt cgagctcgtt gccggccgct tcggcgcgga
      841 ccaacagcac ttcgccgcgc gcatcgggaa gctcttccgc gagcgcgtcg gtggtgtaga
      901 cggggggcgt ccagacctcg ccgtagcctg agccgtgcag cgcacgggcg gttcccggcc
      961 cgatcgcgcc gatgtgcgcg ggatcgaacg gcacgtccag ctcgtgaacg cgatccatca
     1021 cgatgcgcac gccgttgacg ctggtgaaga ccgcccattc gaacgtggtg gcatcacgga
     1081 tggcggcgtc gatgtcgacg ctgttttcgg gtgcctggat ctcgatcgcg ggcaccacga
     1141 ccggctcggc gccgcgttcg cgcagcaggc cggcgagcgc atcggcctgg tcgcgaggac
     1201 gcagcaccag caccgatcgt ccggacaggt tcatgcgatc acctccagcg cgccggccgc
     1261 gatcagcgcg gcggcgatct tcgtgccggc cacgaccgga tcgtcgtcgt cgacggactc
     1321 gtcgatgcga accacccggc gtccgtcggc cgagacggcg ccgccgcgca cccgcatcac
     1381 gcgatcgtcg ccggcttcgg caaggatccc gaccggcagc atgcagccgc cgcccaactc
     1441 gcgcagcgcg gcgcgttcgg tctcggcgca tcgtcgcgtc gccgggtcgt cgatcgcggc
     1501 caccagcgcg gcgacctcgt gatcgtcgtc gcggatctcg acggcgagcg ccccttgcgc
     1561 gggcgcgggc agcatgtcgc gggcgtcgat gatctcgtcg ggctcgatgc ccaaacgaat
     1621 cagcccggcc gcggccagca aggccgcgtc gtagtcgccg ccacggacct tggccacgcg
     1681 cgtgtctacg ttgccgcgga tggaggttgg aatcacgtcg ggccgcaccg ccagaagctg
     1741 cgcggcgcgg cgcgggctgg acgtgcccac gcgcgcgccc gccggcaagg ccgccagggt
     1801 cgagcccacc agcgcctcac gcggatcggt gcggaccggc accgccgcga cgcgcagtcc
     1861 gtccaccggc tcggtcggaa cgtctttgaa cgagtgcacg gcgatgtcga tccggccggc
     1921 gcgcaacgcc tcctgaagtt ccttgacgaa caggccgtca ccggccggcg cgctgatgct
     1981 cgtgtccccg tgcgtccgga tcagccgctc gtcgatgcgt gtgccgggcg ccgcgatgcg
     2041 cagccgtgcg atgaccgagc cggtctgcgt tcgtgccagc gcgctgccgc gcgtgcccgc
     2101 gatcagcgtc ctcattcctg ctccaactcg aagatgcgcc gcaccgtgcg ctcggtggtt
     2161 tcgtcgccgt cgcgcatcgc cagggtcggt c
//
LOCUS       DATLFO010000323         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7867, whole genome shotgun sequence.
ACCESSION   DATLFO010000323 DATLFO010000000
VERSION     DATLFO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7867"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(729..1025)
                     /locus_tag="VM841_13115"
     CDS             complement(729..1025)
                     /locus_tag="VM841_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HVL91166.1"
                     /translation="MIRVVLPAHLRMLARIQGEVEVDVDGTPTPRSVLDAVEAQYPML
                     RGTIREHGTLRRRAYVRFFACNEDLSHDSPDEPLPEKVVRGEEPFMVVGAMSGG"
     gene            complement(1022..2137)
                     /locus_tag="VM841_13120"
     CDS             complement(1022..2137)
                     /locus_tag="VM841_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HVL91167.1"
                     /translation="MSGVRVLVGTRKGAFILTADGARQDWKVEGPLFGGWEMYHLKGS
                     PADPNRIYASQSTSWFGQLVQRSDDGGATWQPVGNEFAYEGDPGTHQWYDGTPHPWEF
                     ARVWHIEPSPSDPDLVYAGVEDAALFRSADGGTTWQEVAGLRAQDTVHQWQPGAGGLC
                     LHTIIIDPTDEERIRIAISAAGVFGTDDAGKTWRPMNRGLQSEGIPDPEAEVGHCVHH
                     MAMHPSRPATLFMQKHWDIMRSDDAGETWREVSGNLPTDFGFVIDVDANDPETIFVVP
                     IKSDSEHYPPEGKLRVYRSRSGGEEWEPLTKGLPQSNCYVNVLRDAMAVDAMDPCGVY
                     FGTTGGAVYVSPDGGDSWSSIVQDLPAVLSVEVQTLP"
     gene            complement(2199..2477)
                     /locus_tag="VM841_13125"
     CDS             complement(2199..2477)
                     /locus_tag="VM841_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVL91168.1"
                     /translation="MPGTAEIFLFRRAAREFGDAWPSGVPLQIHTMQDDEMGDVAEAR
                     ALAEAVDGAELFLYPGDWHLFTDRSIAAFDEAAAKLVMERVLRFLAAV"
     gene            complement(2540..2866)
                     /gene="erpA"
                     /locus_tag="VM841_13130"
     CDS             complement(2540..2866)
                     /gene="erpA"
                     /locus_tag="VM841_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HVL91169.1"
                     /translation="MTIKVSESAATKVRELLEREAPEQPMALRIAVQPGGCSGLRYAL
                     YFDDAVSDKDQTIEEHGVKVVVDKMSAPYLSGATIDYIDSLQQSGFTIDNPNAQSSCA
                     CGDSFH"
     gene            complement(2973..>3456)
                     /locus_tag="VM841_13135"
     CDS             complement(2973..>3456)
                     /locus_tag="VM841_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HVL91170.1"
                     /translation="DSCMTTHPKVQRVVEAAAAAGIAADVREFPDGTRTAGDAARAVG
                     SDVAAIVKTLVWIADGEPVIAMVSGANRVDPAKLAAAAGAAEARRATAEEAREATGFA
                     VGGTPPFGHSRSIRFFIDPTLLALPEVWVAAGTPTTVFAVEPNALQKATGAAAADVAE
                     "
BASE COUNT          595 a         1148 c         1126 g          587 t
ORIGIN      
        1 ggcgggatct attcctactt cttctgcctc ggacgccaga agggcaacgg ctgcgatcag
       61 ccctatctct cgatggacgt cgtcgaagag caggtcgccg agttttaccg gacgcacaaa
      121 cttgagcccg aactaggaga acggcttcgc cacgagtttc gagccgaact ccacgccggc
      181 cggaagcgcc ttgaggagga agctcaacgt caggcgattc ggctggagaa tttgaaggat
      241 cagcgcaaga agattctcta cctgcattac aacgacgcca tcgccatgga gctgttcgaa
      301 gaagaacaga cccgcatcac caatgagatc gccaaggcca aggagatcat cggcaaggcc
      361 gaagaagagt tcgcggtcgt cgaggaaggg tttgaccggg tattcgacat cctcgaagac
      421 ttcgaacgca tctaccgcct ttgcgatgac gccggcaagc gccgcctcaa ccgggcgttc
      481 tggtcgagga tctacgtcga tgacgaaaag aaggttgctg tccaggtagc ggacccggcg
      541 gacccgattg agctcgcgcc gttctacgcc gccgtcgagg aagatccttc gttgccgatt
      601 acagccgccg agcgaacggc tatcaaggct ctcagaaagg caaagaaccc ggccctgatt
      661 tcttcaggtc cgggttcgaa cgttgccatt ctggtggatg tgaacggatt cgaaccttcg
      721 cggcgaagtc agccgccgga catggctccg acaaccataa acggctcttc ccctcgcacg
      781 actttctccg gtagcggctc atcgggtgag tcgtgcgaga ggtcttcgtt gcaggcgaag
      841 aagcggacgt aggcgcggcg gcggagagtg ccgtgctcgc ggatggtgcc gcgcagcatt
      901 ggatactgcg cctccactgc atccagcacg gaccgcgggg tcggcgtgcc gtcgacgtcg
      961 acctcaacct ctccctggat gcgcgcgagc atccgcaggt gggccggcag cacgacgcgg
     1021 atcatgggag tgtctgcacc tcgaccgaga gcacggccgg caggtcctgc acgatcgatg
     1081 accacgaatc cccgccgtcg ggcgagacgt agaccgcccc gccggtcgtg ccgaaataca
     1141 cgccgcacgg atccatcgca tcgacggcca tcgcgtcgcg gagcacgttg acgtaacaat
     1201 tgctctgcgg caggcccttc gtgagcggct cccattcttc gccgccgctg cggctgcgat
     1261 acacgcgcag cttgccctcg ggcggatagt gctcggagtc gctcttgatc ggcacgacga
     1321 agatcgtctc gggatcgttg gcgtccacgt cgatgacgaa cccgaagtcg gtcggcaggt
     1381 tgccgctgac ctcgcgccag gtctcgcccg cgtcgtcgct gcgcatgatg tcccaatgct
     1441 tctgcatgaa caacgtcgcg ggacgcgacg gatgcatcgc catgtgatgc acgcagtgac
     1501 cgacctcggc ctccggatcg gggatgccct ctgactgcag tcccctgttc atcggtcgcc
     1561 aggtcttgcc ggcgtcgtcg gtgccgaaca cgccggcggc cgagatagcg atgcggatgc
     1621 gctcttcgtc ggtcgggtcg atgatgatcg tgtgcaggca cagcccgccg gcgcccggct
     1681 gccactggtg cacggtgtcc tgcgcgcgca ggccggcgac ctcttgccag gtcgtgccgc
     1741 cgtcggccga gcggaacagc gcggcgtcct cgacgccggc gtacaccagg tcgggatcgg
     1801 acggcgaggg ctcgatgtgc cagacgcgcg cgaactccca cggatgcggc gtgccgtcat
     1861 accactggtg cgtgcccgga tcgccttcgt aggcgaactc gttgcccacc ggctgccagg
     1921 tcgcgccgcc gtcgtcggac cgctggacca gctggccgaa ccagctcgtc gattgcgagg
     1981 catagatgcg gttcgggtcg gccggcgatc ccttgaggtg atacatctcc cagccgccga
     2041 acagcgggcc ctcgaccttc caatcctggc gagcaccgtc cgccgtcagg atgaacgcgc
     2101 ctttgcgcgt ccccaccaaa acccgcactc cgctcatcgc gccgcctttc gctttttgga
     2161 tgttcgcggg caccatatcg ccgggcgccg acggcgggct agacggcggc caggaagcgg
     2221 aggacccgtt ccatcaccag cttggcagca gcttcgtcga acgccgcgat gctgcgatcg
     2281 gtaaacagat gccagtctcc cggatacagg aacagctcgg cgccatcgac ggcctcggca
     2341 agcgcgcgcg cttcggccac gtcgcccatc tcgtcgtcct gcatcgtgtg gatctgcaac
     2401 ggcacgccgg acggccaggc gtcgccgaac tcgcgagcgg cacggcggaa cagaaagatc
     2461 tcggccgtgc cgggcaccct actcccgaaa cggtggaagg gcggccactc ggccgccctt
     2521 ccgtggtgaa ctgaaacgct tagtggaacg aatcgccgca ggcgcaggag ctctgcgcgt
     2581 tcgggttgtc gatggtgaac ccggactgct gcagcgagtc gatgtagtcg atggtggcgc
     2641 ccgacagata cggggcgctc atcttgtcga cgacgacctt cacaccgtgc tcttcgatcg
     2701 tttggtcctt gtcgctgacc gcgtcgtcga agtagagggc gtaccgaagg cccgagcatc
     2761 cgccgggctg caccgcgata cgcagcgcca tcggctgctc cggcgcctcg cgttcgagca
     2821 gctccctgac cttggtcgct gcggactcgg aaaccttgat cgtcatcgat cctcctaaag
     2881 ctggaaaccg gcttcttcag tatacggccg caaagggggc cgggaataga tcaacgccct
     2941 gaccaggccg aatgttccct cttccggggg acctattcgg cgacgtcggc ggccgcggcg
     3001 ccggtggcct tctgcagcgc gttcggctcg acggcgaaca ccgtcgtcgg tgtgccggcc
     3061 gccacccaga cctcgggcaa agcgagcaac gtcgggtcga tgaagaagcg aatcgagcgg
     3121 gaatgtccga acggcggcgt tccgcctacc gcgaacccgg tcgcctcgcg tgcttcctcg
     3181 gcggtggcgc ggcgagcctc ggcggctccg gccgcggccg cgagcttggc ggggtccacc
     3241 cggttcgcgc cgctgaccat cgcgatgacc ggctcgccgt cggcgatcca gaccaaggtc
     3301 ttcacgatcg cggcgacgtc gctgccgacc gcgcgcgcgg catcgccggc cgtgcgagtg
     3361 ccgtccggga actcgcggac gtcggcggcg atgccggccg cggctgcggc ttccacgacg
     3421 cgctggacct tcgggtgcgt ggtcatgcac gaatcc
//
LOCUS       DATLFO010000324         5298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7869, whole genome shotgun sequence.
ACCESSION   DATLFO010000324 DATLFO010000000
VERSION     DATLFO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7869"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..816)
                     /gene="tatC"
                     /locus_tag="VM841_13140"
     CDS             complement(103..816)
                     /gene="tatC"
                     /locus_tag="VM841_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVL91171.1"
                     /translation="MTVIEHLEEFRSRIVKAFLAFVVGSAIAWTLYSSLLELLMAPLD
                     EGGRIGGVTVEGLNVGGIVTGFLVRLKVSIFFGLIFALPVILWQLWRFIVPGLKGNER
                     KYGVFFVAGSLGLFGVGVFVAFLVLPQAIGFLLQFAQAPLKPLIFVDQYLSFVSFMVL
                     AFGISFEFPLLLMFLAGMGMISSKKLSKWRRQAIFASAIVAAVATPSQDPYSMLLMAL
                     PLYILYEVSILVIRFVMRK"
     gene            complement(886..1119)
                     /locus_tag="VM841_13145"
     CDS             complement(886..1119)
                     /locus_tag="VM841_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HVL91172.1"
                     /translation="MNVLAIFQQIGMPEILIILAVALFLFGAKRLPEIGKSLGKGIRE
                     FKTGVKGLGDDVREGMEDDAEPQRTKAKESSDG"
     gene            complement(1212..1475)
                     /locus_tag="VM841_13150"
     CDS             complement(1212..1475)
                     /locus_tag="VM841_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91173.1"
                     /translation="MAVIWGLPNWVWYVLAGFALAGFLIIARVLVMLFAAFRELFESL
                     ELSQRMLTAALESTRSEMEKMQESMARIGNKSVEGGDPAWKDW"
     gene            complement(1517..1885)
                     /locus_tag="VM841_13155"
     CDS             complement(1517..1885)
                     /locus_tag="VM841_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91174.1"
                     /translation="MPIHPAKNKGMEDAVTTIKATCPTCGEVELTPDDIELRVCNFAP
                     ASYYTFECPMCFVAVRKPADDRVIQLLISGGVHAVVWEYPAEVSERPEGPPLTHDDLL
                     DFHLLLDQPDWFERLASVAS"
     gene            complement(2125..2730)
                     /locus_tag="VM841_13160"
     CDS             complement(2125..2730)
                     /locus_tag="VM841_13160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVL91175.1"
                     /translation="MCTSVTLPVAPSHNQGPAARIAALIAGALVLAGIAVPIKSGRPP
                     APPAPKTAAADVRSADVEILSLERASKDPVYVGTDGERITAERIRAFLVRQRSPMADY
                     ARVIVSSGIRYGVDPRVIVAISGVESTYGRYSNGYNAWGWNSGKARWPSWSVAIERFT
                     KALGSAYRSLRHGRFAAASRTYCPPCGARWGRQALAIFNHI"
     gene            2946..4355
                     /locus_tag="VM841_13165"
     CDS             2946..4355
                     /locus_tag="VM841_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91176.1"
                     /translation="METRQRRETMIERARWHLWAVTFVIISALVAAVVYVSGNEAAEI
                     STLLPGGRLRLALGLLIIGFLAYVLDRERNLRRLQERLTEERVESTRLVTRLEYLTEL
                     QRERDANAALLEGAADGVAVVDTDLRLLRFNAAMQDLTGVAAAHAVGAYAPAILRFAS
                     PNGVALSDSAYPLRRGFDDGDAVAGMELRMQVSDGSERWVSGTFSPLRDGDGGRPALL
                     LAVLRDITEAKEMQALQRDFVSIVSHELRAPLTAIKGFAKTLLQRGEALPPETRANFL
                     TTVNAQADRLARLVDDLLQVSRIDARRLRMDLQPVCASEMVESLLEQFSGKWSRPVSV
                     EIPDRLPLVSADRPKLEEILINLIDNAIKYSPPDTPVRVAAREGEGEVEFAVEDRGFG
                     ITAEDAAKLFQKFQRLSTPATRDVGGTGLGLYIVKGLVDAMGGRVWVESAPGDGSTFA
                     FTVPVAGEAAGERIEKVGA"
     gene            4352..>5298
                     /locus_tag="VM841_13170"
     CDS             4352..>5298
                     /locus_tag="VM841_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVL91177.1"
                     /translation="MIRSKRNDDPSAPRTARSRSRSRDFEALLEASRAANRAAGNLER
                     ALTTILDLAIDLVGADEGSVMLVEEPAATMRIVAARGLPASVRRDTRIVIGRGIAGSV
                     AQAGRPTLLRTPLDVSRFEGYVEKARPIHSALSVPMRARERVIGVLNLNRMTPEGQLD
                     EEDLQIATLFAEHAALAVTAAASLASSQRTAGELESLRAATARLGRSLDLDTVADAAL
                     TEALAIAGTPSGLLVLGNDTRVDLARYRGMSRDGIRAALRTPGFAARLAATEPLVVRS
                     LPADPVFAPLAADLGAHALAVAPLIGPDGAAGGAVGSDQR"
BASE COUNT          878 a         1809 c         1809 g          802 t
ORIGIN      
        1 atcagcggct gcggcttgac ggccggcttc gggaccggcg ctttgggagc gccgcggccc
       61 gacctcgacc ggcggcggga cttcgacttg atcgccacgt cgctacttcc tcatcacgaa
      121 acgaatcacc aggatggaga cctcgtaaag gatatacagc ggcagggcca tcagaagcat
      181 cgagtacgga tcctgcgagg gcgtcgcgac cgccgcgacg atcgccgagg cgaagatcgc
      241 ctgccggcgc cacttcgaca gcttcttcga cgagatcatg cccatgccgg ccaggaacat
      301 cagcagcagc gggaactcga aggagatccc gaacgcaagc accatgaacg acacgaacga
      361 caggtactgg tcgacgaaga tcagcggctt gaggggcgcc tgcgcgaact gcagcaggaa
      421 gccgatcgct tgcggcagca cgaggaaggc gacgaacacg ccgacgccga acaggcccag
      481 cgagcccgcc acgaagaaga caccgtactt tcgctcgttg cccttgagcc ccgggacgat
      541 gaatcgccag agctgccaca ggatgacggg aagggcgaag atcagcccga agaagatcga
      601 gaccttcaag cggacgagga aaccggtgac gatgccgccg acgttgagcc cttcgacggt
      661 cacgccgccg atccggccgc cttcgtccaa gggcgccatg agcagctcga gcagcgacga
      721 gtacaacgtc cacgcgattg cggatccgac gacgaacgcg aggaacgctt tgacgatgcg
      781 ggagcggaac tcttcgagat gctcgatgac ggtcatccgc ccgtcgggat tcgtggggcg
      841 gcgacgcaaa cgcatcatcg gaggtgggac aagcgagcgg actgcctagc cgtccgagga
      901 ttccttcgcc ttggtccgct gcggttcggc gtcgtcctcc atgccttcgc ggacgtcgtc
      961 gccgagaccc ttgacgcccg tcttgaactc acggatgccc ttgcccagcg attttccgat
     1021 ctcgggcagc cgcttggcgc cgaacaggaa gagggcgacg gccaggatga tcaggatctc
     1081 gggcatccct atttgctgga agatcgcgag gacgttcacg gtgggcctcc ttcgccgcgc
     1141 gaagcgcacg actgttcaag tctaccatcc ggccttcgtc gctctgggac gcggaaacga
     1201 gccggtcgat ttcaccagtc tttccaggcg gggtccccgc cctcgacgga cttgttcccg
     1261 atgcgggcca tgctctcctg catcttttcc atctcggaac gtgtcgattc aagcgccgcg
     1321 gtcagcattc gctgggacag ctcgagcgac tcgaagagct cacggaaggc cgcgaacagc
     1381 atgacgagca cgcgagcgat gatcaggaag ccggcgagcg cgaagcccgc gagcacgtac
     1441 cagacccagt tgggaagccc ccagatgacc gccacaccgg cattctagtg ccgcgcgccg
     1501 ccgacgacgg cggagcctag gaagcgacgc tggcgagacg ctcgaaccag tcgggctggt
     1561 cgagcagcag gtggaagtcg agcaggtcgt cgtgggtgag cggcggccct tcaggacgct
     1621 cggacacctc ggccgggtac tcccacacga ccgcgtgcac gccgccggag atcagcagct
     1681 ggatgacccg gtcgtcggcc ggcttgcgca cggccacgaa gcacatcggg cactcgaagg
     1741 tgtagtagga cgcaggggcg aagttgcaga cgcgcagctc gatgtcgtcc ggcgtgagct
     1801 cgacttcacc gcaagtgggg caggtcgcct taatagttgt caccgcgtcc tccatgcctt
     1861 tgttcttcgc cgggtgtatc ggcatcgcga gcgccggacc ttgagatgtt ccttcgttcg
     1921 cggatgccgt tcggtttcat cactccaacg aaggggattc gcggcaggtt ccgggcgcgt
     1981 tatggaaata ccggactttg cgtcggtgat aaaacgccca gtgcggcgcg gccgggccaa
     2041 tgccgcgatc tcgtggtttc gctttgctcc gaaggccggg aaccgcaggt cagaccggct
     2101 gcgggacgca cgcagcccgc cggcctagat gtggttgaag atcgcgagcg cctgacggcc
     2161 ccagcgggcg ccgcacggcg ggcaatacgt ccgggatgcg gcggcgaagc gtccgtggcg
     2221 cagcgagcga tacgcggacc cgagcgcttt ggtgaagcgc tcgatcgcga cgctccacga
     2281 cggccaccgg gccttgccgg agttccagcc ccaggcgttg tagccgttcg agtaccttcc
     2341 ataggtcgat tcgacgccgg agatcgccac gatgacgcgc ggatcgaccc cgtagcggat
     2401 gccggacgag acgatcacgc gggcgtagtc ggccatcggc gagcgctggc ggacgaggaa
     2461 ggcccggatc cgctcggccg tgatgcgctc gccatcggtg ccgacgtaca ccggatcctt
     2521 cgaggcgcgt tccagggaca ggatctcgac gtcggcggag cggacgtcgg ccgccgcggt
     2581 cttgggagcc ggcggcgccg gcggccgccc cgacttgatc gggaccgcga ttccggcaag
     2641 cacgagcgcg ccggcgatca gcgcggcgat tctcgctgcc ggaccctggt tgtgcgacgg
     2701 cgcgacgggg agcgtgacgc ttgtgcacat atcggacacg aggcgccact ttggggggcg
     2761 gggggtgttt ctgtcaactt ggcctaatca cgaattcacg agcgctgaaa gagcgcccgc
     2821 cgacgtcaaa ttcgggcaga gttggcccgt ccgccgccga agacaccctc cgcacgtcaa
     2881 gacggccccc tccccgtgcc gatattcaag cggatcaggg acggccgacg acgtggagga
     2941 cggcgatgga gacgcggcag cgccgcgaga cgatgatcga gcgtgcgcgc tggcacctgt
     3001 gggccgtcac cttcgtgatc atcagcgcct tggtcgccgc ggtcgtgtac gtgagcggca
     3061 acgaggccgc cgagatctcg acgcttctgc ccggtggacg gcttcgactg gcgctgggcc
     3121 tgctcatcat cgggttcctc gcctacgtgc tggaccgcga gcgaaacctc cgccgcctcc
     3181 aagagcggct caccgaggag cgggtcgaat ccacccggct cgtgacgcgc ctcgaatatc
     3241 tcaccgagct gcagcgcgag cgtgatgcga acgcggcctt gctcgaaggc gccgccgacg
     3301 gcgtggccgt ggtcgatacc gacctgcggc tccttcgctt caacgccgcg atgcaggacc
     3361 tgaccggggt cgccgccgcc cacgccgtcg gtgcctacgc accggcgatc cttcgcttcg
     3421 cctctcccaa cggggtcgcg ctgagcgact ccgcctatcc gctgcgccgc ggcttcgacg
     3481 acggcgatgc cgtcgccggc atggaacttc ggatgcaggt ctcggacggg agcgagcggt
     3541 gggtcagcgg aaccttctct cccctccggg acggcgacgg cggccgtccg gcactgctgc
     3601 tggccgtcct tcgggacatc accgaggcca aggagatgca ggcgctgcag cgggatttcg
     3661 tctcgatcgt ctctcacgag ctgcgcgccc cgctgaccgc catcaaaggg ttcgccaaga
     3721 ccttgcttca gcgaggagag gctctcccgc ccgagacgcg ggccaacttc ctgaccacgg
     3781 tcaacgccca ggccgaccgg ctcgcccggc tcgtggacga tctgctgcag gtctcacgca
     3841 tcgacgcgcg ccgtctgcgg atggaccttc agccggtgtg tgcgtccgag atggtcgaga
     3901 gcctgctcga gcagttctcg ggcaaatggt cgcggccggt atccgtggag atcccggacc
     3961 ggctgccgct ggtgtcggcc gaccggccca agctcgagga gatcctgatc aacctgatcg
     4021 acaacgcgat caagtactcc ccgcccgaca cgccggtgcg cgtcgcggcc cgcgagggcg
     4081 agggcgaggt cgaattcgcg gtcgaggacc gcggcttcgg catcacggcc gaggacgccg
     4141 ccaagctgtt ccagaagttc cagcgcctgt ctacgccggc cacgcgcgac gtcggcggaa
     4201 ccggtctcgg gctctacatc gtcaagggcc tggtcgacgc catgggcggc cgggtctggg
     4261 tcgagtccgc ccccggagac ggctcgacgt tcgccttcac cgtgcccgtg gccggcgagg
     4321 ccgccgggga gcggatcgag aaggtaggcg catgatccgt tcgaagcgaa acgacgaccc
     4381 gtcggcgcct cgcacggcgc gatcgcggtc gcgcagccgc gacttcgagg cgctgctcga
     4441 ggcctcgcgc gcggccaacc gcgccgccgg aaacctggag cgcgcgctga ccacgatcct
     4501 tgacctggcc atcgatctgg tgggcgccga cgaaggctcg gtcatgctcg tcgaagagcc
     4561 ggccgcgacg atgcgcatcg tcgcggcacg gggcctgccg gccagcgtcc gacgcgacac
     4621 ccggatcgtg atcgggcgcg gcatcgcggg ctcggtcgcg caagcgggcc ggccgacgct
     4681 gcttcgcacg ccgctggacg tgtcccgctt cgagggctac gtcgagaaag cccgtccgat
     4741 ccattcggcg ttgtcggttc cgatgcgcgc ccgcgagcgc gtcatcggcg tgctcaacct
     4801 caatcgcatg acgcccgagg gacagctcga cgaagaggac cttcagatcg cgaccttgtt
     4861 cgccgagcat gccgcgctcg ccgtgaccgc cgcggcgtcg ctcgcctcgt cgcagcgaac
     4921 cgccggcgag ctggagtcgc tccgtgcggc caccgcccgg ctcgggcgca gcctcgatct
     4981 ggacaccgtg gccgacgccg cgctgaccga agcgctcgcg atcgccggaa cgccctccgg
     5041 cctgctcgtg ctcggcaacg acacgcgggt cgatttggcc cgctatcgcg gcatgtcccg
     5101 cgacggcatc cgggccgccc ttcgcacgcc cggcttcgcc gcacggctcg ccgccacgga
     5161 gccgctggtg gtgcgcagcc tcccggccga cccggtcttc gccccgctcg cggcggacct
     5221 gggcgcgcac gcgctggccg tcgcgccgct gatcggaccc gacggcgccg ccggcggcgc
     5281 cgtcgggtcc gatcagcg
//
LOCUS       DATLFO010000325         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7894, whole genome shotgun sequence.
ACCESSION   DATLFO010000325 DATLFO010000000
VERSION     DATLFO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7894"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VM841_13175"
     CDS             <1..558
                     /locus_tag="VM841_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HVL91178.1"
                     /translation="GVLCGLNAVLRVPGAIGGASLVFVLPIVAGAVFGPAFGFLLGAL
                     SFAASGLVTAGIGPWLPFQMFAAGWIGAGAGLLRPAVERVRPVVAVAILCAYGYAAGF
                     FFGVVTNLWFWPSLAAGDASIGWRPGMGAAEAAGAFRRFYLLTSLAWDAGRAVGNVVL
                     IALLGAPALRLLRRWRDRMWTPPTS"
     gene            604..1407
                     /gene="trxA"
                     /locus_tag="VM841_13180"
     CDS             604..1407
                     /gene="trxA"
                     /locus_tag="VM841_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVL91179.1"
                     /translation="MSVQDVTDAAFAADVLERSKTTPVVVDFWATWCGPCRQLSPTIE
                     RVADDFRDTVVALKMDVDANPQTAGMLGIQSIPTVVAFKDGKPVDAFLGAQPENMVRD
                     FFARLAPSEDDRVVEETAALPDDEAEPVLRGVLSRDPGHRGAVLRLAAIVGMRGETAE
                     AKALLARIPEDEDVRRVAAQVEMASVSSADLGSLAAAAASDVTARIEYGRALAAAGRH
                     AEALEQLMSAFHDGERDPAREAMLDVFALLGDADPLVVRYRRELASALF"
     gene            1470..3290
                     /gene="typA"
                     /locus_tag="VM841_13185"
     CDS             1470..3290
                     /gene="typA"
                     /locus_tag="VM841_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HVL91180.1"
                     /translation="MTEIRNVALVAHVDHGKTTLVDAMLRASGVLRANTTAPDRVMDS
                     NDLEREKGITIFAKAAQIPWKGITINLVDTPGHADFGGEVERALAMVDGIILIVDAAE
                     GPLPQTRYVLQKALHRGLPSIVLVNKIDRKDARAEEVLDEIYQLYFDLEAQDHHIDFP
                     VFSAIARDGKVVKDIAEPPEDADLSPLLDAIVERIPAPSGDSTAPLQALVTNLDASDY
                     LGRLAIGRVVQGTLRVDDSIALCRDDGSTERRKLTALERFEGLSRVRVDEIYAGDLFV
                     AAGLPDVEIGDTLADPDAPVALGRLIVDEPVLRMTFGVSTSPMSGREGKFLTSRHLKE
                     RLDREVLGNVSIRVTPTDSPEVIEVAGRGELQLAVLIETMRREGYEFQVSRPEVITRE
                     IEGVLHEPLERGIVDVPDEHVGTVSQNLAPRKGKVVDIRPGDAGRSIVTFEAPARGLI
                     GFRTLLLTATRGTALLHLHAAGYIPWVGDLPHRQGGAMVADRIGKSTAYALDSLQPRG
                     VLLIGPGEEVYEGMVVGEHSRPDELVVNVTREKQKTNIRTHASDEAIKLEPPRQLTVE
                     TAIEFIAGDELVEVTPQSLRVRKRHLDRRVRERAAKAQPH"
BASE COUNT          484 a         1151 c         1174 g          507 t
ORIGIN      
        1 ggcgtgctgt gcgggctcaa cgccgtgctc cgcgtgcccg gcgccatcgg cggcgcgtcg
       61 ctggtgttcg ttcttcccat cgtggcgggc gcggtgttcg gtcccgcgtt cggcttcctg
      121 ctcggcgcgt tgtcgttcgc ggcgtcgggt ctggtcacgg ccggcatcgg gccgtggcta
      181 ccgttccaga tgttcgcggc cggatggatc ggcgccggcg cgggcctgct gcggcccgcc
      241 gtcgagcgag tccgccccgt cgtcgcggtg gcgatcctgt gcgcgtacgg ctacgcggcc
      301 ggcttcttct tcggcgtggt gacgaacctg tggttctggc cctccctcgc ggcgggcgac
      361 gcctcgatcg gatggcgtcc gggaatgggc gccgccgagg cggccggcgc cttccgccgc
      421 ttctatctgc tgacctcgct cgcatgggac gcaggccggg ccgtcggcaa cgtggtcctg
      481 atcgcccttc tcggcgcgcc ggcgctgcgc ttgctccgcc gctggcgcga ccggatgtgg
      541 acgccaccca cgtcgtagcc gtcgtccgcg tggtagcggc cgccgttcga tacgctggaa
      601 cccatgagcg tccaagacgt aaccgacgcg gccttcgcgg ccgatgtgct cgagcgatcc
      661 aagaccacac cggtcgtggt ggatttctgg gccacctggt gcggcccgtg ccgtcagctt
      721 tcccccacca tcgagcgggt cgccgacgac ttccgcgaca cggtcgtcgc gttgaagatg
      781 gacgtggacg cgaacccgca gaccgcgggc atgctcggca tccagtcgat cccaaccgtg
      841 gttgcgttca aggacggtaa gccggtggat gcgtttctcg gcgcgcagcc cgagaacatg
      901 gtgcgcgact tcttcgcgcg gctcgctccg tccgaggacg accgcgtcgt cgaagaaaca
      961 gcggcgctgc cggacgacga agccgagccg gtccttcgcg gggtgctctc acgcgacccc
     1021 ggtcatcgcg gcgccgtgct ccggctcgcg gccatcgtcg ggatgcgcgg cgagaccgcc
     1081 gaggcgaagg ccttgcttgc ccgcatcccc gaggacgagg acgtgcgccg cgtcgccgct
     1141 caggtcgaga tggcctcggt gtcgagcgcc gacctcggct cgctcgcggc cgcggccgct
     1201 tcggacgtca ccgcgcggat cgagtacggc cgggcgctgg ctgccgccgg ccgtcacgcc
     1261 gaggcgctcg agcagctcat gagcgcgttc cacgacggcg agcgcgatcc ggcacgtgag
     1321 gccatgctcg acgtgttcgc cctgctcggc gacgccgacc cgctggtcgt gcgctaccgc
     1381 cgggagctgg cgagcgcttt gttctaaaag gcaacccccg caggatccgc gagcggcgcg
     1441 ggtcgcgcgg aggtactctt ggcgggctca tgaccgagat ccgcaacgtt gctttggtgg
     1501 cgcacgtcga ccacgggaag accaccctgg tcgatgcgat gctgcgcgcc tcgggcgtgt
     1561 tgcgcgcgaa cacgaccgcg ccggaccggg tcatggactc caacgacctc gagcgcgaga
     1621 agggcatcac gatcttcgcc aaagccgcac agatcccgtg gaagggcatc acgatcaatc
     1681 tggtggacac gcccggccac gccgacttcg gaggagaggt cgagcgagcc ctggcgatgg
     1741 tggacggcat catcctgatc gtggacgcgg ccgaagggcc gctgccgcag acccgctacg
     1801 tgctgcagaa ggcgctgcac cgcgggctgc cctcgatcgt gctcgtcaac aagatcgacc
     1861 gcaaggacgc gcgcgccgaa gaggtgctcg acgagatcta ccagctctac ttcgacctcg
     1921 aggcccaaga ccatcacatc gactttccgg tcttcagcgc gatcgcccgc gacggcaagg
     1981 tcgtcaagga catcgccgag ccgcccgagg acgccgacct gtcgccgctg ctcgacgcga
     2041 tcgtggaacg cattcccgcg ccgtcgggcg actcgaccgc gccgctgcag gcgctggtga
     2101 cgaacctgga cgcgagcgac tatctcggcc gcctcgcgat cggacgcgtt gtgcaaggga
     2161 cgctgcgcgt ggacgactcg atcgcgctgt gccgcgacga cgggtcgacc gagcgtcgca
     2221 agctgaccgc gctcgaacgg ttcgaaggct tgagccgcgt gcgcgtcgac gagatctacg
     2281 cgggcgacct gttcgtagcc gcgggcctgc ccgacgtcga gatcggcgac acgctcgccg
     2341 acccggacgc gccggtcgca ctcggacggc tgatcgtgga cgagcccgtg ctgcgcatga
     2401 ccttcggcgt gagcaccagc ccgatgtcgg gccgcgaagg caagttcctg acgagccggc
     2461 atctgaagga gcggctcgac cgcgaggtgc tcggcaacgt gagcattcgc gtcacgccga
     2521 ccgactcgcc cgaggtcatc gaggtcgccg gccgcggtga gctgcagctc gccgttctga
     2581 tcgagacgat gcggcgcgaa ggctacgagt tccaggtgtc gcgtcccgag gtgatcacgc
     2641 gcgagatcga aggagtgctg cacgagccgc tggagcgcgg gatcgtggac gtgcccgacg
     2701 agcacgtcgg cacggtgtcg cagaacctcg cgccacgtaa gggcaaggtc gttgatatcc
     2761 gtccgggcga tgcgggccgc tcgatcgtca ccttcgaggc gccggcgcgc gggttgatcg
     2821 gcttccgcac gctgctgctg acggcgaccc gcggcaccgc gctcctgcat ctgcacgccg
     2881 cgggctacat cccgtgggtg ggcgacctgc cgcaccggca gggtggcgcg atggtggcag
     2941 accggatcgg caagtccacc gcctatgcgc tcgactcgtt gcagccgcgc ggcgtgctgc
     3001 tgatcgggcc cggcgaagaa gtctacgaag gaatggtggt cggggagcat tcgcggccag
     3061 acgagctcgt cgtcaacgtc acgcgcgaga agcagaagac caacatccgc acgcacgctt
     3121 cggacgaggc gatcaagctg gagccgccgc gccagctgac cgtcgagacg gcgatcgagt
     3181 tcatcgccgg cgacgagctg gtcgaggtca cgccgcagtc gcttcgggtc cgcaaacgcc
     3241 atctggaccg ccgcgtgcgc gagcgggccg ccaaggccca gccccactaa ccgccgcgag
     3301 cgggccgcca aggccc
//
LOCUS       DATLFO010000326         5970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_117360, whole genome shotgun sequence.
ACCESSION   DATLFO010000326 DATLFO010000000
VERSION     DATLFO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117360"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VM841_13190"
     CDS             complement(<1..1052)
                     /locus_tag="VM841_13190"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HVL91181.1"
                     /translation="MTQKLEGPAYVLEARSFLQNPSQDDLMALALKMPNAKPTKYGNV
                     NVTTRVDSRSSKSTFIATDDPAAHEGHATISREEFSRIAGLQDDFIRDQDMVVIDGFI
                     GNDPEFKVPARLIIEQANANIAGMQDLLYFKEGVPADHQPAVTVIYTPNLPAAGYPND
                     RLIAVDLDNGLTRVLNSDYFGESKKGGLRMWNRIVVDRGGLPMHAGCKTVPTEEGDKT
                     FLIVGLSGTGKTTTTFTRQNDSKPVQDDFIALMPGGKVHGTENGCFAKTFALSKEYEP
                     TIYGAVTQPKSYLENVFQTEDGDLDFFNESFTQNGRAVFGMEALGWFQDAREAGTVDY
                     LLILNRNNNIIPAVAR"
     gene            complement(1154..1363)
                     /locus_tag="VM841_13195"
     CDS             complement(1154..1363)
                     /locus_tag="VM841_13195"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HVL91182.1"
                     /translation="MGVLNTIELEGVSDESWGAAAREALREAAKTIRHIVKMDVLETS
                     CSVREDSILEYRTSVRLYFEVEPSR"
     gene            1482..2483
                     /gene="thiL"
                     /locus_tag="VM841_13200"
     CDS             1482..2483
                     /gene="thiL"
                     /locus_tag="VM841_13200"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HVL91183.1"
                     /translation="MDGKAVSDLGEFGLIERLRRRLADAAAAGVEIGPGDDAAAVDTG
                     PSTLLAADMLVEGIHFDLAFSSLADAGYKAVAVNVSDIAAMGGMPRYLLVSLGLPEGV
                     SVTAVDALYDGMLEAAGETGCAIVGGDVTGAPQIVVSVSVAGEPGPAGVLRRSGAQEG
                     DVLCVTGALGVAGAGLALMRAAADPRAVALLDAYPALAAAHRRPLPRVAAGPAAAEAG
                     AHAMIDVSDGFARDAAHIAEESGLGLVVDGASLPVTAGVSDAAGFLGVEAAVLAASGG
                     DDYELAIAVPPDRVEAVRSAIAPLPLTVAGAFGGTDRVFVHARERIPLRELGWEHFA"
     gene            2465..3277
                     /gene="thiD"
                     /locus_tag="VM841_13205"
     CDS             2465..3277
                     /gene="thiD"
                     /locus_tag="VM841_13205"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HVL91184.1"
                     /translation="MGALRVTEVALSIAGSDSGGGAGIQQDLKVFEALGVWGATAVTA
                     ITVQDTHGVRDVHSVPWELVAAQIDAVAADLRPSAAKTGMLPDGACVRGVCDAIERHR
                     LGPLVVDPVLAASDGTGLMDPAALDLVRERLVPLAAVITPNLAEAAVLTGLAVDSPGS
                     QETAARALVELGAAAALVTGGHLEGDAIDILFDGETVTAFPGPRIPGGPVHGTGCVLS
                     AAITAGLARGRPLRSAVGEAKELVTGAIERARSIGGGARVADAAWRSRPPHH"
     gene            complement(3345..3536)
                     /gene="rpmB"
                     /locus_tag="VM841_13210"
     CDS             complement(3345..3536)
                     /gene="rpmB"
                     /locus_tag="VM841_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HVL91185.1"
                     /translation="MAAVCEICKRGPGFGMQVSHSHRRSPKRWNPNIQRVRAKVNGAT
                     KRLNVCTRCIKAGKIQRAG"
     gene            3688..5247
                     /locus_tag="VM841_13215"
     CDS             3688..5247
                     /locus_tag="VM841_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HVL91186.1"
                     /translation="MTFGPNEFKRAIVAALGSLRAHQASIDEANVFPVPDGDTGTNML
                     LTMSAAAEALGSPDPFAAAPRAALVGARGNSGVVLAQWLRGFCETAGPGDPAAVVRGL
                     RRAAELAYTAVLEPVEGTILTAARAAADAAGGPTAAEIFESAAAGARAAVDRTPDLLP
                     VLKQAGVVDAGALGLWIVLDAFGASLSGREPAAAPGLASSAAPRAMARESGSSRFGYE
                     VQYLLEAADEVIEPLRAELGRIGDSVVVSGGGGLWNVHVHTNDTGEAVEIGRAVGTVR
                     TASVTAFADRTGGPRSIPVARAAAPVSVVAVVAGEGFARLFAELGAGVVIDGGSTVNP
                     SVGEIADAIRRCPAADVIVLPNNDDAIPAAMQACELVTDRRAVVVGTHDLAHGVTTML
                     AYGDARDLETNAAQMRQAASVAASAKVIVAARDATTPAGTVRAGQAIAFCGADVAAIE
                     QDPVSAVVRVVTTMDDEEPIEVVTVFAGATAPAGERSALEKALRDTLPGVDVDVRDGG
                     QPVERYLIAVE"
     gene            5244..>5970
                     /locus_tag="VM841_13220"
     CDS             5244..>5970
                     /locus_tag="VM841_13220"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVL91187.1"
                     /translation="MSMPTADASAGPLDRPLSLARLSGLSGPATRKLAAKGIVTIRDL
                     IEQVPRRYVDLSRTKPIGDLKLGEEATIVGRIYRVDGRYIRGSKRHMLVAKIGDGTGF
                     VEAVWWNQPFRAKQILEGIDVAVAGRFERRQGRLQVTGAFFVERLGGPGESVHTGRII
                     PVHPATEGVSPAMLRRAIHDALAAFGPEIDDPLPEELLDRLDLASRNEAFRQIHFPDS
                     LAAKNAARRRLAFEELFVLSSGLA"
BASE COUNT          795 a         2064 c         2182 g          929 t
ORIGIN      
        1 aatcgcgcga ccgccgggat gatgttgttg ttgcggttca ggatcagcag gtagtccacc
       61 gtgccggcct cgcgcgcgtc ctggaaccag ccgagcgcct ccatgccgaa caccgcgcgg
      121 ccgttctgcg tgaacgactc gttgaagaag tccagatcgc cgtcctcagt ctggaacacg
      181 ttctccaggt aggacttcgg ctgcgtgacc gcgccgtaga tcgtgggctc gtactccttc
      241 gaaagcgcga acgtctttgc gaagcagccg ttctcggtgc cgtggacctt gccgcccggc
      301 atcagcgcga tgaagtcgtc ctgcacgggc ttggagtcgt tctgccgcgt gaaggtcgtc
      361 gtggtcttgc cggtgcccga cagacccacg atcaggaacg tcttgtcgcc ctcctcggtc
      421 ggaaccgtct tgcagcccgc gtgcatcggc aggccgccgc ggtccacgac gatgcggttc
      481 cacatgcgca ggccgccctt cttggactcg ccgaagtagt ccgagttgag cacgcgcgtc
      541 aggccgttgt ccaggtccac ggcgatcagg cgatcgtttg gatagcccgc ggcgggaagg
      601 ttcggcgtgt agatgacggt cacggccggc tgatggtcgg cgggaacgcc ctccttgaag
      661 tagagcaggt cttgcatgcc ggcgatgttg gcgttggcct gctcgatgat caggcgcgcg
      721 ggaaccttga actccgggtc gttgccgatg aagccgtcga tgacgaccat gtcctggtcg
      781 cgaatgaagt cgtcctgcag ccctgcgatg cgggagaact cttcgcggga gatcgtggcg
      841 tgaccctcgt gtgcggccgg gtcgtcggtg gcgatgaagg tggacttcga cgagcgagag
      901 tccacacggg tggtgacgtt gacgttgccg tacttcgtcg gcttggcgtt cggcatcttc
      961 aatgccagcg ccatcagatc gtcctgcgac gggttctgca ggaacgaccg cgcctccaga
     1021 acgtatgcag ggccttcgag cttctgggtc acgagaatgc gcctcccgga gagttcgtga
     1081 aagctttcac aaggatgata ctccccgaat cgggcgcggt aaaactgccc ggagcgcgga
     1141 cccggaatcg cgtttagcgg ctgggctcga cctcgaaata caggcgcacc gaggtgcggt
     1201 attcgaggat cgaatcctcg cggacggagc agctcgtctc gagcacgtcc atcttcacga
     1261 tgtggcggat cgtcttggcg gcctcgcgaa gcgcctcgcg tgcggccgcg ccccacgact
     1321 cgtccgagac gccttccagc tcgatcgtgt tcaggacgcc catcgactgc ctcccgtgcc
     1381 cccgccgtct tcgccaacgg gccgcattct tgcgcctgaa cggcccgggg gcaaggtgcc
     1441 gcggtgtttt tccggcttcc gagggccgta tgctgccgga aatggatggc aaggcagtct
     1501 ccgatctcgg ggagttcggc ctgatcgagc ggctgcgccg gcggctcgcc gacgccgcgg
     1561 ccgccggggt cgagatcggc ccgggggacg acgcggccgc agtggatacc ggcccgtcca
     1621 cgctgctggc ggccgacatg ctcgtcgagg gcatccactt tgatcttgcc ttctcctcac
     1681 tcgccgacgc cggatacaag gccgtcgccg tgaacgtctc ggatatcgcg gcgatgggcg
     1741 ggatgccgcg gtacctgctg gtgtccctcg gcctgcccga aggcgtctcg gtcaccgccg
     1801 tcgatgccct ctacgacggc atgctcgagg ccgcgggcga gaccggctgc gcgatcgtcg
     1861 gcggggacgt gaccggcgcg cctcagatcg tggtgtcggt ctcggtcgcg ggcgagccgg
     1921 gcccggccgg ggtgctgcgc cggtccgggg cgcaggaggg cgacgtgctc tgcgtgaccg
     1981 gggcgctggg cgtcgccggc gccggcctcg cgctgatgcg cgcggccgcc gatcccaggg
     2041 ccgtggcgct cctggacgcc taccccgcgc tcgccgcggc ccatcggcgg cccctgccgc
     2101 gcgtggcggc cggacccgcg gccgccgagg cgggcgcgca cgcgatgatc gacgtctcgg
     2161 acggcttcgc ccgtgacgcc gcgcacatcg cggaggagtc ggggctgggg ctcgtcgtcg
     2221 acggcgcgtc gctgccggtc acggccgggg tctccgacgc ggccgggttc ctgggcgtcg
     2281 aggccgcggt gctggcagca tcgggcggag acgactacga gctcgcgatc gcggtgccgc
     2341 cggaccgggt cgaagccgtg cgctccgcga tcgctccgct cccgctgacg gtggccggcg
     2401 cgttcggcgg aaccgaccgc gtcttcgtgc acgcccggga gcgcatcccg ctgcgcgagc
     2461 tcggatggga gcacttcgcg tgaccgaggt cgcgctgagc attgcgggct ccgattccgg
     2521 cgggggagcg gggatccagc aggacctgaa ggtcttcgag gcactcggcg tctggggggc
     2581 gaccgccgtg acggcgatca cggtgcagga cacccacggc gtccgcgacg ttcactccgt
     2641 gccgtgggag ctcgtggccg cgcagatcga cgcggtcgcc gcggatttgc ggccgtccgc
     2701 cgccaagacc ggcatgctgc ccgacggcgc ctgcgtccgg ggggtctgcg acgcgatcga
     2761 gcgtcaccgg ctcggcccgc tggtcgtgga tccggtcctg gcggcgagcg acggcaccgg
     2821 gctgatggat ccggccgctc tcgatctcgt gcgcgagcgg ctggtgccgc tcgccgcggt
     2881 gatcacgccg aacctggccg aggccgctgt gctcaccggc ctggccgtcg actcgcccgg
     2941 ctcgcaggaa accgccgccc gagcgctggt cgagctgggg gcggccgcgg cgctcgtgac
     3001 cggcggccac ctcgagggcg acgcgatcga catcctgttc gacggcgaga ccgtcacggc
     3061 gttcccgggg ccgcgaatcc ccggcgggcc cgtgcacggc accggctgcg tcctgtcggc
     3121 ggcgatcacc gcgggcctgg cccggggacg gccgctgcgg tccgccgtcg gcgaggccaa
     3181 ggagctcgtc accggcgcga tcgagcgagc ccgctcgatc ggcggcggag cgcgcgtggc
     3241 cgacgccgcc tggcgaagca ggcctcccca tcactgaaat gtccaaccac gtccgtgaaa
     3301 cgaaaaacgc cgcccggagg cggcgggtag ccacgaggat gaggtcagcc ggcgcgctgg
     3361 atcttgccgg ccttgatgca gcgcgtgcag acgttgagcc gcttggtcgc gccgttcacc
     3421 ttcgcgcgca cccgctggat gttcgggttc cagcgcttgg ggctgcggcg atgggaatgc
     3481 gaaacctgca tcccgaagcc gggaccacgc ttgcagatct cgcacacggc agccatagcc
     3541 cgcgcattgt agcgacggac ctccgggcgg acaacccgat gaatcggaat cgaccgggtg
     3601 cggccgcccc ggtccgttgc cggcgtcggg attgtccgca ttcccggccg tgagggccgg
     3661 tcggcccgcc ccgttaggat tccggccgtg accttcgggc cgaatgagtt caaacgagcg
     3721 atcgtcgcgg cgctcgggtc gcttcgtgcc catcaggcct cgatcgacga ggcgaacgtc
     3781 ttccccgtcc ccgacggcga caccggcacg aacatgctgc tcaccatgtc ggccgcggcc
     3841 gaagcgctcg gttcgccgga ccctttcgct gctgcgccgc gcgccgctct ggtcggggcc
     3901 cgcggcaact ccggcgtcgt actggcccag tggttgcgcg ggttctgcga gacggccggc
     3961 cccggcgacc cggccgcggt cgttcgcggg ctgcggcgag ccgccgagct tgcctacacc
     4021 gcagtgctcg agcccgtcga gggaacgatc ctgacggcgg cccgcgccgc ggcggatgcg
     4081 gccggcggac cgacggccgc cgagatcttc gagtcggccg cggccggcgc ccgcgcggcg
     4141 gtcgatcgca ccccggatct gcttccggtc ttgaagcagg ccggcgtggt ggacgccggc
     4201 gcactcggcc tctggatcgt gctcgatgcg ttcggcgcat cgctttccgg ccgcgaaccg
     4261 gccgcggcgc ccggcctcgc gagcagcgcg gcgccgcggg cgatggcccg cgaatccggc
     4321 tccagccggt tcggctacga ggtgcagtac ttgctcgagg cggccgacga ggtgatcgag
     4381 ccgctgcggg cggagctggg ccggatcggt gattcggtcg tggtgtccgg cgggggcggg
     4441 ctgtggaacg tgcacgtgca caccaacgac accggcgagg ccgtggagat cggccgcgcc
     4501 gtcggcacgg tacggaccgc gtcggtgacc gcgttcgccg atcggaccgg cgggccgcgc
     4561 tcgatcccgg tcgcacgcgc cgcggcgccg gtctcggtcg tggcggtcgt cgccggcgaa
     4621 ggcttcgcgc ggctgttcgc cgaactggga gccggcgtgg tgatcgacgg cggctcgacg
     4681 gtgaacccgt cggtcggtga gatcgccgac gcgatccggc gttgcccggc ggccgatgtg
     4741 atcgtgctgc cgaacaacga cgacgccatc ccggccgcga tgcaggcgtg cgagctcgtc
     4801 acggaccggc gcgccgtggt ggtcggcact cacgacctcg cgcacggcgt cacgacgatg
     4861 ctcgcctacg gcgatgcgcg ggacctcgag accaacgccg cgcagatgcg ccaggcggcc
     4921 tcggtggccg ccagcgcgaa ggtgatcgtc gcggcccggg atgccacgac gcccgcgggc
     4981 accgttcgtg ccggccaggc gatcgcgttc tgcggcgccg atgtcgcggc gatcgagcag
     5041 gatccggtct cggccgtggt gcgcgtggtg acgacgatgg acgacgagga gccgatcgag
     5101 gtcgtgacgg tcttcgcggg tgcgaccgct cccgccggcg agcgatccgc gctcgagaaa
     5161 gcgcttcgcg acacgctgcc cggcgtagac gtggacgtgc gcgacggcgg gcagcccgtc
     5221 gagcgttatc tgatcgcggt cgaatgagca tgccaacggc cgatgcgtcg gcgggtccgc
     5281 tggaccgacc gctctcgctc gcgcgcctga gcggcctgag cggtccggcg acgcgcaagc
     5341 tcgccgcgaa aggcatcgtg acgatccgcg acttgatcga gcaggttccg cgccgctacg
     5401 tggacctctc gcgcacgaag ccgatcgggg acctcaagct cggcgaggag gcgacgatcg
     5461 tcggacgcat ctatagggtc gacggccgct acatccgcgg gtcgaagcgc cacatgctcg
     5521 tggccaagat cggcgacggc accggcttcg tcgaagcggt gtggtggaac cagccgttcc
     5581 gggccaagca gatcctcgag ggcatcgacg tcgcggtggc gggccgcttc gagcgccgcc
     5641 aaggccgtct gcaggtgacc ggcgcgtttt tcgtcgagcg cctcggcggc cccggcgaga
     5701 gcgtgcacac cggacggatc atcccggtgc atcccgcgac cgaaggcgtc tcgccggcga
     5761 tgctgcgccg ggccatccac gacgccctcg ccgcgttcgg ccccgagatc gacgacccgc
     5821 tgccggagga gctgctcgac cggctcgacc ttgcctcccg caacgaggcc ttccggcaga
     5881 tccacttccc cgactcgctc gccgccaaga acgccgcgcg ccgccgtctg gcgttcgagg
     5941 agctgttcgt tctttcgtcc ggcctggcga
//
LOCUS       DATLFO010000327         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_97992, whole genome shotgun sequence.
ACCESSION   DATLFO010000327 DATLFO010000000
VERSION     DATLFO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97992"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VM841_13225"
     CDS             <1..290
                     /locus_tag="VM841_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVL91188.1"
                     /translation="VGAAGVVSVAGHLAGNRIAEMIAAHDKGQIDVAAQINRDLVPLV
                     DALFCTTSPIPLKAALAMVGLPGGPVRLPLVDATERETSIMRAALEKLGIL"
     gene            381..2702
                     /locus_tag="VM841_13230"
     CDS             381..2702
                     /locus_tag="VM841_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HVL91189.1"
                     /translation="MAKKKRGRSPGGKTRSSKSKGGGIGARLKAVSDALSRAFGPHLS
                     DAYGIGLALLAVLCVLGIWLHKAGPVGAFLELTARGLFGRGGFLAPVLLGYLAYAFFT
                     TRPGPDRPRVVVGLSLVALGALGLWHLAQGTPDVTAGAETLRDAGGLVGALVAGPLRR
                     LVAPPGAAITLLAVLGFGVLVLTRTPVRRIGETLAAVRANLAGRVRAKAAALAARRAE
                     ARPPRAAEPEPERGPEPELEPEPQPIVPAAPPLPTEPIIQVLLPDDDVQGDDVQGDGD
                     EHDGGEPAAMARRSGQYRRPSIDLLRKSAAPAPAGKSVNDTIRAIEQTLRQFDVDASV
                     ARHTRGPTVTRFEVELGEGVKVNRVVSLANDIAYALATPDVRIIAPIPGRSAIGIEVP
                     NLEREVVALRELLTCTKAKTDDHPLIVGLGKDISGEPIVINLAKMPHLLIAGATGSGK
                     STCINTMLVSILMRAKPEELRMILVDPKRVELTHYNGMPHLLSPVITHPKRAAEALNW
                     VVKEMENRYDDLAASGHRQVDSYNQAVREGRVKKHGLPVTETMPYILVVIDELADLMM
                     VAPRDVEDSICRIAQMARAVGIHLLVATQRPSVDVVTGLIKANIPSRIAFATSSQADS
                     RVILDQVGADKLIGFGDMLFLPAAMSKPMRVQGAFVSDPEVEAVATHCRRESDPDYVE
                     GVVSVTTSQASSEGEGDDDDLMTQAMELVVRSGLGSTSMLQRKLKVGFARAGRIMDLL
                     EERGVVGPSVGSKPREVLMSIEELEEQRAGATA"
     gene            2798..>3092
                     /locus_tag="VM841_13235"
     CDS             2798..>3092
                     /locus_tag="VM841_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91190.1"
                     /translation="MRIPAVILALLLAVAVPASAEEPYRQQALFEWDRLVIDVLVLPP
                     GHGQIANDGGVLPAGTADLHPLANSYSRAAERGIALWPAAIRAHGPAWLAEGLR"
BASE COUNT          462 a         1087 c         1087 g          456 t
ORIGIN      
        1 ccgtcggcgc cgcgggcgtg gtgagcgtcg cgggccacct ggccggcaac cggatcgctg
       61 agatgatcgc cgcgcacgac aagggacaga tcgacgtcgc cgcgcagatc aaccgggatc
      121 tcgtgccgct ggtcgacgcg ctgttctgca ccacgagccc gatcccgctg aaagcggcgc
      181 tcgcgatggt cggccttccc ggaggaccgg tgcgtctgcc gctcgtcgac gcgaccgagc
      241 gcgagacgtc catcatgcgg gctgcgttgg aaaaactcgg catactgtag ggaccggcgc
      301 gagccggtgg ttgcgggctg cttgcggtcc gctgatcgat ctccgcacat cgaggcagcg
      361 aaacgaagcc gggaggctcg gtggccaaga agaagcgggg gcgatcgcct gggggcaaga
      421 cccgctcttc gaagtcgaag ggcggcggga tcggagcgcg gctcaaggcc gtttccgatg
      481 cgttgtcgcg cgcgttcgga ccgcacctgt ccgacgcgta cggcatcggc ttggcgctgc
      541 tcgccgtcct gtgcgtgctc ggcatctggc ttcacaaagc cggtccggtc ggcgcgttcc
      601 tcgagctgac cgcgcgtggg ctgttcggtc gcggcggatt cctggcgccg gtccttctcg
      661 gctatctcgc ctacgcgttc ttcaccacaa ggcccggccc cgaccgtcct cgcgtcgtcg
      721 tcgggctgtc gctcgtggcc ttgggcgcgc tcggcttgtg gcatctcgcg caaggcacgc
      781 ccgacgtgac cgcaggcgcc gagacgctgc gcgacgccgg cggtctcgtc ggagcgctcg
      841 tcgccggccc gctgcgccgg ctcgtcgcgc cgccgggcgc ggcgatcacg ttgctcgccg
      901 tcctcgggtt cggggttttg gtgctcaccc ggacgccggt ccgccggatc ggcgagacgc
      961 tcgccgccgt gcgcgcgaac ctcgcagggc gcgtgcgagc caaggccgcg gcgctggccg
     1021 cgcgccgggc cgaggcccgt ccgccgcgag cggccgagcc cgaacccgaa cgcgggccgg
     1081 aacccgagct cgagccggag ccgcagccca tcgttccggc tgcgccgccg ctgccgaccg
     1141 agccgatcat ccaggtcctc ctgcccgacg acgacgtcca gggcgacgac gtccagggcg
     1201 acggcgacga gcacgacggg ggcgagccgg cggcgatggc ccgtcggagc ggacagtacc
     1261 ggcggccgtc gatcgatctg ctgcgaaagt ccgccgcgcc ggcgccggcc ggcaagtccg
     1321 tcaacgacac gatccgcgcg atcgagcaga cgcttcgcca gttcgacgtg gacgcctcgg
     1381 tcgctcgtca cacacgcggc ccgacggtta cccgcttcga ggtcgagctc ggcgaaggcg
     1441 tgaaggtcaa ccgggtcgtg agcctcgcca acgacatcgc ctacgcgctg gcgacgcccg
     1501 acgtgcgcat catcgccccg atccccggcc gcagcgcgat cgggatcgag gttcccaacc
     1561 tcgagcgcga ggtcgtcgcg ctgcgcgagc tgctcacgtg cacgaaggcc aagaccgacg
     1621 atcatccgct gatcgtgggc ctcggcaagg acatcagcgg cgagccgatc gtgatcaacc
     1681 tcgccaagat gccgcacctt ctgatcgccg gcgcgaccgg ctcgggcaag tccacgtgca
     1741 tcaacacgat gctggtgtcg atcctgatgc gggccaagcc ggaggagctg cgcatgatcc
     1801 tggtcgatcc caagcgcgtc gagctgaccc actacaacgg catgccgcat ctgctgtctc
     1861 cggtcatcac gcatcccaaa cgcgcggcgg aggcgctcaa ctgggtggtc aaggagatgg
     1921 agaaccgcta cgacgatctc gccgcgagcg gccaccggca ggtggactcc tacaaccagg
     1981 ccgtgcgcga agggcgcgtc aagaagcacg gcctgcccgt caccgagacg atgccctaca
     2041 tcctggtcgt catcgacgag ctcgcagacc tgatgatggt ggcgccgcgc gacgtcgagg
     2101 actcgatctg ccgcatcgcg cagatggccc gcgcggtcgg catccatctg ctggtcgcaa
     2161 cccagcgccc cagtgttgac gtggtcaccg gcctgatcaa ggccaacatt cccagccgga
     2221 tcgcgttcgc gacctcctcg caggccgata gccgcgtgat cctggatcag gtcggcgcgg
     2281 acaagctcat cggcttcggc gacatgctct tccttccggc cgcgatgtcc aagccgatgc
     2341 gcgtgcaggg cgcgttcgtg tcggatcccg aggtcgaggc cgtcgcgacg cactgtcgcc
     2401 gcgagtccga tcccgactac gtcgagggtg tcgtgtcggt gacgacgagt caggcctcgt
     2461 ccgagggtga aggcgacgac gacgacttga tgacgcaggc gatggagctg gtcgtgcgca
     2521 gcggcctcgg atccacgagc atgctgcagc gaaagctcaa ggtcgggttc gcgcgtgcgg
     2581 gccgcatcat ggatctgctc gaggagcgcg gcgtcgtcgg tccgtcggtc ggctcgaagc
     2641 cgcgcgaggt gctgatgtcg atcgaggagc tggaggagca gcgcgccggc gcgaccgcct
     2701 gagccgggcg gacaatcgcg cccctgagcg tcgaagcggc accaacgaac cttcgtccga
     2761 aagggagcgg gtcgggacgc atcgaaacgc tgtgtccatg cgcatacctg ccgtgatcct
     2821 ggccctgctc ctcgccgtcg ccgtgcccgc gagcgcggag gagccctacc gccagcaggc
     2881 gctgttcgag tgggaccggc tcgtgatcga cgtgctggtg ctgccgcccg gtcacggcca
     2941 gatcgccaac gacggcggcg tgctgccggc cggcaccgcg gaccttcacc cgctggcgaa
     3001 ctcctacagc cgcgcggccg agcgaggcat cgcgctgtgg cccgccgcga tccgggctca
     3061 cgggcccgcc tggctcgccg agggccttcg gc
//
LOCUS       DATLFO010000328         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_98008, whole genome shotgun sequence.
ACCESSION   DATLFO010000328 DATLFO010000000
VERSION     DATLFO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98008"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2961
                     /locus_tag="VM841_13240"
     CDS             <1..>2961
                     /locus_tag="VM841_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91191.1"
                     /translation="FDRSGRVTTYCADMYGERVSGVDMTRLCDQPFPTAPYHVASLSI
                     VPGGDVFAGGLGLFRFSRGGWYREPNANGYLISISMGSNNEGFVASSGRSLGAGGMIR
                     SSSTTIGHWTASPKRSAISRWAQPQNRLLRGMALAEDGSGRAIAVGAYGSGIIFSGAA
                     WDSIDSVTGQVLNAAAWPAGAEPWAFGSSGIILRLRNLRWQVTDSPTTSSLYSAAFRS
                     PAEGVAVGLGGTILVYQGGRWRDDSNDAIASDLYSVITTPSGYLAVGSDGAVVEGRPG
                     SWKRRAEARTLLKRQDSPVAPTLYTATRLGNRIVMGGQDSALIVREADGRIAPFEAPL
                     QGTVMSLSSAGSSLYASVTPGEEKWRGEAAAAQRGTVMRYAGGRWADIGLERRTTLLR
                     DEVDPSRFEDPIYDLRMASESAGWGAGGSPADFKDLVEGHFRSEETSAIYKIAVGGDP
                     RQPASSAPLDPPKPGVTFAAFGESWCGVGLCSSAVGTGTMADEVSLQIRDEINKAAAQ
                     PGGPKFVLFTGNMRRVGIPEELEQFRNYLKGFDVPVFVAPGNRDRFAGFEVVLPTGGR
                     TQNGSHDYWKNVFAKQPAPWGHAASPRNIAPVSIGNAEPIRDAARTHYAFDYMEGGKR
                     MMRVVVVDSSTRSYGTFEQQNPPEDQETWLPTVLAEAQDVLRIPTVVLTNQAPFIPSE
                     QQVPNWSNQGHQQHFEATVAATRVSAVITGGPRMNARDTIKGVVPLYVVGGGGAPLGR
                     DLDLQQGSVQLDPPSKEPSDGYYHAWHLFRIDPQDRNPITQQARVQSQAFPVVESLAM
                     HSFAGAQIPAGQTTTITAMARMIAGGFSDPDQAKAQTLEHGTLKFFCGYKGQGNGQCI
                     PDSTVLPRFRFYSEDPTIADFLERDELYPSAPKSMGAGFARDPDGEDGLLCVFKLGKV
                     GINAVAGMHRARIEIEGIPGNGPCVEKEVLGKKIIQPVFPRVPEQVPAAKQQGFSRTP
                     PHNGELVLRRGAAE"
BASE COUNT          464 a         1038 c         1015 g          444 t
ORIGIN      
        1 acttcgaccg cagcgggcgc gtcaccacct actgcgcgga catgtacggc gagcgggtgt
       61 cgggcgtcga catgacccga ctctgcgacc agccgttccc gaccgcgccg tatcacgtcg
      121 cgtcgctttc catcgtgccc ggcggcgacg tgttcgccgg cggtctgggt ctgttccggt
      181 tctcccgcgg cggctggtac cgggagccga acgcgaacgg atacctgatc tcgatctcga
      241 tgggctcgaa caacgaagga ttcgtcgctt cgtccggacg ttcgctgggc gcgggcggca
      301 tgatccgctc cagctcaacc acgatcggcc actggacggc atcgccgaag cggtccgcga
      361 tctcgcgctg ggcgcagccg cagaaccggc ttcttcgcgg catggccttg gccgaggacg
      421 gatccggacg tgcgatcgcg gtcggcgcct acggctccgg catcatcttt agcggggctg
      481 cgtgggacag catcgactcg gtgaccggcc aggtgctcaa tgccgccgcg tggccggccg
      541 gcgccgagcc ctgggccttc ggctcctccg gcatcatctt gcgtctgcgc aacctgcgct
      601 ggcaggtgac ggactcgccg accacgtcca gcctgtattc ggccgcgttc cgcagcccgg
      661 ccgaaggcgt cgccgtcggg ctcggcggca cgatcctggt ctatcagggc ggccgttggc
      721 gcgacgactc gaatgacgcc atcgcgagcg acctgtactc ggtgatcacc acgccgtccg
      781 gctacctcgc cgtcggatcc gacggcgcgg tggtcgaagg acggccggga agctggaagc
      841 ggcgcgccga ggcccggacg ctgctcaagc gtcaggacag cccggttgcg ccgacgctgt
      901 acaccgcgac gcggctcggc aaccggatcg tgatgggcgg acaggactcg gcgttgatcg
      961 tgcgcgaggc ggacggacgc atcgcgccgt tcgaggcgcc gctgcaggga accgtgatgt
     1021 cgctgtcgag cgcgggctcc tcgctgtacg ccagcgtgac gcccggcgag gagaagtggc
     1081 gcggcgaggc ggccgcggcc cagcgcggaa ccgtcatgcg ctacgccggc ggccgttggg
     1141 ccgacatcgg gctcgagcgc cgcacgacgc tgcttcgcga cgaggtggac ccgagccgtt
     1201 tcgaagaccc gatctacgat ctgaggatgg cgtccgagag cgccggctgg ggcgcgggcg
     1261 gctcgccggc cgatttcaag gatctggtcg aaggtcactt ccgcagcgag gagacctcgg
     1321 ccatctacaa gatcgccgtc ggcggcgacc ctcgccagcc tgcctcctcg gcgccgctcg
     1381 acccgcccaa gcccggcgtg acgttcgccg ccttcggcga gtcgtggtgc ggcgtcggcc
     1441 tgtgctcgtc cgcggttggg accggcacca tggccgacga ggtctcgctg cagatccgcg
     1501 acgagatcaa caaggccgcc gcgcagccgg gcggacccaa gttcgtgctg ttcaccggca
     1561 acatgcggcg cgtcggcatt cccgaggagc tcgagcagtt ccgcaactac ctcaagggat
     1621 tcgacgttcc ggtcttcgtg gcgccgggca atcgcgatcg gttcgccggg ttcgaggtcg
     1681 tgctgcccac gggcgggcgt acccagaacg gatcgcacga ctattggaag aacgtcttcg
     1741 ccaagcagcc ggcaccgtgg ggtcacgccg cgtcgccgcg aaacatcgcg ccggtctcga
     1801 tcggcaacgc cgagccgatc cgcgacgcgg cgcgcacgca ctacgccttc gactacatgg
     1861 aaggcgggaa gcggatgatg cgcgtggtcg tggtggactc gtccacgcgc tcctacggca
     1921 ccttcgagca gcagaacccg ccggaggatc aagagacctg gctgccgacg gtgctcgccg
     1981 aagcgcagga cgttctgcga atccccacgg tcgtgctcac caaccaggcg cccttcatcc
     2041 cgtccgagca gcaggtgccg aactggtcga accagggcca ccagcagcac ttcgaggcca
     2101 ccgtggccgc gacgcgcgtg agcgcggtca tcaccggcgg cccgcgcatg aacgcccggg
     2161 acacgatcaa gggcgtcgtg ccgctctacg tcgtcggcgg cggcggcgcg ccgctcggcc
     2221 gcgacctcga cctgcagcag ggctcggtcc agctggatcc gccgtcgaag gagccgagcg
     2281 acggctacta tcacgcctgg cacctgttcc gcatcgatcc gcaggatcgc aacccgatca
     2341 cgcaacaggc ccgtgtccag tcgcaggcgt tccccgtggt cgagtcgctc gccatgcact
     2401 cgttcgccgg cgcccagatc ccggccgggc agaccaccac catcaccgcg atggcgcgca
     2461 tgatcgccgg cggcttctcg gatccggatc aggccaaggc ccagacgctc gagcacggaa
     2521 cgttaaagtt cttctgcggc tacaagggcc agggcaacgg ccagtgcatc ccggactcga
     2581 cggtccttcc gcgcttccgc ttctacagcg aggatccgac gatcgccgac ttcctcgagc
     2641 gtgacgagct gtatccgagc gcgcccaagt cgatgggggc cgggttcgcg cgcgaccccg
     2701 acggcgagga cggcctgctg tgcgtgttca agctcggcaa ggtcggcatc aacgccgtgg
     2761 ccggcatgca tcgcgcgcgc atcgagatcg aaggcattcc cggcaacggg ccttgtgtcg
     2821 agaaggaggt gctcggaaag aaaatcatcc agccggtctt cccgcgcgtc cccgaacagg
     2881 tccccgccgc gaagcagcaa ggcttcagcc gcaccccgcc gcacaacggc gagctcgttc
     2941 tgcggcgggg tgcggctgaa g
//
LOCUS       DATLFO010000329         8353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_78052, whole genome shotgun sequence.
ACCESSION   DATLFO010000329 DATLFO010000000
VERSION     DATLFO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78052"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..315)
                     /locus_tag="VM841_13245"
     CDS             complement(31..315)
                     /locus_tag="VM841_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91192.1"
                     /translation="MIGGPQQPGTQPHDRKAVGLLAALIALALATAIVSMPKIDRDGD
                     RPPSAIPTPTVTPGSPEPSPTETGPETTEPSPTESPSPTVTATATATTGT"
     gene            662..961
                     /gene="rpsF"
                     /locus_tag="VM841_13250"
     CDS             662..961
                     /gene="rpsF"
                     /locus_tag="VM841_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVL91193.1"
                     /translation="MADRRRYELMLILDPRLEESAIQTTVDRYLGFVKDQGGEVAKVD
                     HWGRKRFAFEINHLDEGYYVLADLDTDPATVAELDRQMRLADELVRHKFVRPGKD"
     gene            995..1426
                     /gene="ssb"
                     /locus_tag="VM841_13255"
     CDS             995..1426
                     /gene="ssb"
                     /locus_tag="VM841_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVL91194.1"
                     /translation="MANGNTVTLVGNLTDDPELRYTPQGAAVANFRIAVNHRFKDQAG
                     NWQDGETSYFRINCWRQLAENIAESLTRGTRVVITGRLKMRQWETQEGEKRSVVEVEA
                     DEIGPSLRFATAKVERTSKGSGGGGGGDWDAGAPMDEEVPI"
     gene            1544..1831
                     /gene="rpsR"
                     /locus_tag="VM841_13260"
     CDS             1544..1831
                     /gene="rpsR"
                     /locus_tag="VM841_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVL91195.1"
                     /translation="MPRDRDRDRKGGRSRRDTEGKGGFGRRQKRKVCQFCREKSDWVD
                     YKDVQLLRKFVSDRGKIRARRVTGTCAQHQADVATAVKNAREMGLIPYAVR"
     gene            1828..2274
                     /gene="rplI"
                     /locus_tag="VM841_13265"
     CDS             1828..2274
                     /gene="rplI"
                     /locus_tag="VM841_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HVL91196.1"
                     /translation="MKVILTQEVRALGAPGDVIDVADGYGRNYLIPRGLARLATKGSL
                     KQVEMIRKTRELREVASREKAEDVRNHLQSLKVRVKAKSGDGGRLFGQVTPAAIAEAI
                     VKAGGPKVDKRRLHLDQPIKSLGMHTVHLRVHAEVEAAINVEVQRG"
     gene            complement(2334..2723)
                     /locus_tag="VM841_13270"
     CDS             complement(2334..2723)
                     /locus_tag="VM841_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVL91197.1"
                     /translation="MDTHAVLWWAAGSPLLSKRARSAIKSASRLYVSPLSCWEVARLV
                     NRGRVRLADDPSLWTRALFEQERVEPASTTPEAAVAAAGLPETFPGDPIDRLLYATAA
                     DLRLPLITRDRAIHRLAAQRRDIRTIW"
     gene            complement(2737..2976)
                     /locus_tag="VM841_13275"
     CDS             complement(2737..2976)
                     /locus_tag="VM841_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HVL91198.1"
                     /translation="MERARIVAATDFKQHCLALLDDVSRSGRAIVVTKHGRPVARLVP
                     VEDRSASAFFGSVTLVSDRPEDYYSAGDAWETEDH"
     gene            3077..3562
                     /locus_tag="VM841_13280"
     CDS             3077..3562
                     /locus_tag="VM841_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91199.1"
                     /translation="MTRRSGASTGFWAASVAAVIAVAILLALDVRITSTQSRWIAGAA
                     AITVASALRFLVSPRGAVAIDATAAAGLAAVAIATSAVGGLAFFIAVVFLVTAVLAGA
                     RSERSTHRPIVAKSVLVAALVLLPLALLSPGGAIFVVGPVSIPFIAWAVWSLRRMPAG
                     A"
     gene            complement(3614..4162)
                     /locus_tag="VM841_13285"
     CDS             complement(3614..4162)
                     /locus_tag="VM841_13285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL91200.1"
                     /translation="MSDDPKPWQVLASRYLHQEPPWLVVREDRVALPNGVQIERYWVN
                     EYEAWCNVIAVTPDDRIVMVRQYRHGIGQVHYELPGGVADHGTPEESARTELLQETGF
                     GGGAWSPFMRICANPSLASNWTHTFLAEGVERIAEPDHEPTEDLRIHFMPVRDIPQMI
                     DDGEIVQALHAAALLRYALQRA"
     gene            4411..6546
                     /gene="dnaB"
                     /locus_tag="VM841_13290"
     CDS             4411..6546
                     /gene="dnaB"
                     /locus_tag="VM841_13290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HVL91201.1"
                     /translation="MAIDLTRDREANQVSRVPPHSLDAEQSVLGAMMLSKTAISEVLE
                     ILQPDDFYRSAHQQIATAMRDLFASGEAVDAITVVEEMRRKGTLESAGGAPYIHTLLA
                     GVPTAANAAFYAQIIVEHSTLRRLIDASTRIAQECYDIPPDIDEAINRAGEAIYKVAA
                     RRIHSDFRPIRELLAESMEQLEMLASRDSDITGVSTGFRDIDDRLSGLQRQNLIIVAA
                     RPAMGKTSFVLNVAHNAAMKSNVPVVIFSLEMSQNEIVQRLISSESHVDSSRLRTGHL
                     SEADWTRVSNAVGKLDTAPLYIDDSASISMMEIRAKCQRLKQQQDLGLVIVDYIQLMQ
                     GSRRTESRVQEVSEISRGLKILAKEMDVPVVAVSQLSRQPEQGGGERRPQLSHLRECV
                     TGDTLVCLADGRRLPIEALVGTTPMVLAMDDRGRIVESASDMVWKVGRRPVFSVRLAS
                     GRSIRATAKHRLFGFDGWVRVGMLAAGDRIAISRTLTQPGKVEAWPAGRPAFNAVVSG
                     TSGQRIFRRRIGAFGPRVAQATALTEYLVDRPSNTNVDTIPAAVFDRVRMQMQQRGIS
                     QQAMALARGPAYGGTSHWRFGPSRLVLAENADILDDPVLAELASNDLFWDKVIAVEPA
                     GEEDVYDLTVPGPASWLADGIVSHNSGAIEQDADVVMFVYRDEYYNKESEAKGEAEII
                     VAKHRNGPVGTEKLAFLGQYTKFADLARE"
     gene            6589..6843
                     /locus_tag="VM841_13295"
     CDS             6589..6843
                     /locus_tag="VM841_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVL91202.1"
                     /translation="MNIIWWLIVGFIAGLLARALVPGDDSMGIVGTTVLGLVGSLVGG
                     FLGNLVSDSDGFSPSGLIGSIIGAIIALLIWRAMDRRAHA"
     gene            6874..7575
                     /gene="nth"
                     /locus_tag="VM841_13300"
     CDS             6874..7575
                     /gene="nth"
                     /locus_tag="VM841_13300"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVL91203.1"
                     /translation="MAQAASDRERAREIVRRLARAYRSGHPARPDTSVVGSVVKPPLD
                     ELIFTLLSQHTSDLNRDRAWVSLRERFPAWEQVASARTKSIADAIRIGGLADSKAPRI
                     KAVLREVREREGSYGLERLRRMSDDDVRHYLLSLPGIGPKTAACVLAFSLARPALPVD
                     THVHRLAIRLGFAGPKADAAATQQILESLVPGRSRLATHVRLIAHGRMICTARRPKCG
                     ECVLADLCPSAARLA"
     gene            7583..>8353
                     /locus_tag="VM841_13305"
     CDS             7583..>8353
                     /locus_tag="VM841_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL91204.1"
                     /translation="MRSRTQAGYLELVLLGVLWGSIGVIIEPIRVGAAGIALVRTATG
                     CLLALAYMLLRRRVARLRLRRSAWLLIADGVLLAVHWTLMFEAFKRLSVGTAILLVFI
                     GPVLVAVGAWPLLRERVETRTIGALAVSLGGMALIAIPAWEVQDPAGVVYALLSAVAF
                     AMVVLANKRLTAIYEPATILVWQLGVAALAVAPVALTGTLDGFGEALPRLLMLGALHT
                     AAAGFIYFAAVRVVKVQHVGVLTYLEPATAIAYAWIFLS"
BASE COUNT         1343 a         2748 c         2854 g         1408 t
ORIGIN      
        1 tgccgcactc cgggcaggtg aacgagtgcg tcaggtcccc gtcgttgcgg tggccgtggc
       61 ggtgacggtc ggggatgggc tctcggtcgg agacggttcc gtcgtctcgg gaccggtctc
      121 ggtgggtgac ggctccgggg agcccggcgt cacggtcggc gtggggatcg ccgacggtgg
      181 acggtcgccg tcccggtcga tcttgggcat cgacacgatc gccgtcgcga gcgcgagcgc
      241 gatgagcgcg gcgagcaagc ccacggcctt gcggtcgtgt ggctgcgtcc cgggctgctg
      301 aggcccgccg atcatgcgag gtccttcata ggggaagtcg gtccggcata ggggaagtcg
      361 gtccggccgg gcaatgctac ggcaccccgg cggcggggcc ccgaagggaa gaagacgaag
      421 ggatcgagcc ccgcgagtat ctatttatcc cgattgttcg cgagcctgtc gcgagcccga
      481 ggggagcgtc attccctcgt tgtatggtgc cttcgaaggg acgaattgac gattgtggag
      541 gtgtgctacc ctgagtcgct cgccggccgg ataaccggcg agtgcagtct cacccctgcc
      601 cgaaccgtat ccgttcaaaa gggacgaata cggagggccg cctagaccag aggaggtgag
      661 gatggcggac cggcgccgtt acgaactcat gctcattttg gatcctcgtc tcgaggaatc
      721 cgcgatccag accaccgtcg accgctatct cgggttcgtc aaggaccagg gtggcgaggt
      781 ggccaaggtg gatcactggg gccgcaagcg gttcgcgttc gagatcaacc atctcgacga
      841 gggctactac gtgctcgcgg atctcgacac ggatccggcc accgtcgccg agctcgaccg
      901 tcagatgcgt ctggccgacg agctcgtgcg ccacaaattc gttcgtcccg gaaaggacta
      961 gcccaagcac gtccttcccc gggaggtgaa gacaatggcc aacggaaaca cggtcacgct
     1021 ggtcggaaac ctgaccgacg accccgagct gcggtacacg ccgcagggag cggccgtcgc
     1081 caacttccgc atcgccgtga accaccgctt caaagaccag gccggcaact ggcaggacgg
     1141 cgagacgagc tacttccgca tcaactgctg gcggcagctc gccgagaaca tcgccgaatc
     1201 gctcacgcgc ggaacccgcg tcgtgatcac cggccgcctg aagatgcgcc agtgggagac
     1261 ccaggagggc gagaagcgct ccgtcgtcga ggtcgaagcc gacgagatcg gcccgtcgct
     1321 gcggttcgcg accgccaagg tcgagcggac ctcgaaggga agcggcggag gcggcggcgg
     1381 cgactgggac gcgggcgccc cgatggacga ggaagtgccg atctaacgat cggcccgcag
     1441 aagtgggtcg gaccgcgcac ctgctccggg tgcgcggacg cggcggccgc acggccgtcg
     1501 cgccgccgag tccggcccgg tcgaacagac ggaggacatc gaagtgccga gagatagaga
     1561 cagggaccgc aagggcggcc gcagccgccg cgatacggaa ggaaagggcg gcttcggacg
     1621 ccggcagaag cgcaaggtct gtcagttctg ccgcgagaag tccgactggg tggactacaa
     1681 ggacgtgcag cttctccgga agttcgtctc ggaccgcggc aagattcgcg cccgccgcgt
     1741 gacgggaacg tgcgcgcagc accaggcgga cgtcgccacc gccgtcaaga acgcccgtga
     1801 gatgggcctg atcccctacg cggtccgatg aaagtcatcc tgacgcagga ggtgcgcgcg
     1861 ctcggcgcac ccggcgatgt gatcgatgtc gccgacggct acgggcgcaa ctatctgatc
     1921 cctcgcgggc tggcgcggct tgcgacgaag ggatcgctca agcaggtcga gatgatccgc
     1981 aagacccgcg agcttcgcga ggtcgcaagc cgcgagaagg ccgaggacgt gcgcaaccac
     2041 ctgcagtcac tgaaggtgcg cgtcaaggcc aagtccggcg acggcggacg gctcttcggt
     2101 caggtcaccc cggccgcgat cgccgaggcg atcgtcaagg ccggcggccc gaaggtggac
     2161 aagcgccgcc ttcacctgga ccagccgatc aagtcgctcg gcatgcacac cgtgcatctg
     2221 cgtgtgcacg ccgaagtcga agccgccatc aacgtcgaag tccagcgcgg ctagaacgga
     2281 tcgtcgaagc gaagcgcccg tctcgttttc agaggcgggc gctttccttt cggctaccag
     2341 atcgtccgga tgtcgcgccg ctgggcggcg aggcgatgga tcgcgcggtc gcgggtgatc
     2401 agcggaaggc gaaggtctgc ggcggttgca tacagcagcc ggtcgatcgg atcgcccggg
     2461 aacgtctcgg ggagcccggc cgcggcgacc gccgcttccg gcgtggtgga tgccggctcg
     2521 acgcgttctt gctcgaacag ggcgcgcgtc cacagggatg gatcgtccgc gagccggacg
     2581 cggcctcggt tcacgaggcg tgcgacctcc cagcagctga gcgggctgac gtacagacgc
     2641 gaagcacttt tgatcgccga ccgggctcgt ttcgacagaa gagggctgcc tgccgcccac
     2701 cacaagaccg cgtgggtgtc caacagcagt cctgcgctag tgatcttcgg tctcccaggc
     2761 gtcgcccgcc gagtagtagt cctcagggcg gtccgacacc agcgtcaccg acccgaagaa
     2821 cgccgaggcg gagcgatcct cgaccggaac cagccgggcg accggccgcc cgtgcttggt
     2881 gaccacgatt gcccggcccg accgcgagac gtcgtcgagc agcgccaggc aatgctgctt
     2941 gaaatccgtc gccgccacga tgcgcgcccg ctccatgcgc ccagaataga acccgggcaa
     3001 gaatctgtcc ataaaaactg tccagtttgc cgcgctcgtc ttgctcgggg cttccgccgg
     3061 ccgtatcgtg ggtcgcgtga cgcgacgatc gggtgcctcg accggctttt gggcggcctc
     3121 ggtggccgcc gtcatcgccg tcgcgatcct gctggccctc gacgtgcgaa tcacgtccac
     3181 gcagtcgcgg tggatcgccg gggctgcggc aatcaccgtc gcctccgcgc tgcgtttcct
     3241 cgtgtcgcct cgcggtgcgg tggccatcga cgcgaccgcg gccgctggtc tggcggccgt
     3301 cgccatcgcg acctctgcgg tcggcggtct ggccttcttc atcgcggtgg tgtttctcgt
     3361 gaccgccgta ctggcgggtg ctcgctccga gcgatcgact caccggccga tcgtggcgaa
     3421 gagcgttctc gttgcggcgc tcgtcctgct tcctctggcc ttgctgtcgc cgggtggagc
     3481 gatcttcgtg gtgggcccgg tgagcattcc gttcatcgct tgggcggtct ggtcgcttcg
     3541 ccgcatgccg gccggcgcct aacgggaacg atcccggcat gaacgaactc atcgaaggct
     3601 tcggcgcgaa ggattaggcg cgctgcagcg cgtaacggag cagcgcggca gcgtgaagcg
     3661 cctgcacgat ctcgccgtcg tcgatcatct gcgggatgtc gcggaccggc atgaagtgga
     3721 tgcgcagatc ttcggtcggt tcgtgatcgg gctcggcgat gcgctcgacg ccttcggcca
     3781 gaaaggtgtg cgtccagttg ctggccagac tcgggttcgc gcagatgcgc atgaacggcg
     3841 accacgctcc tccgccgaag ccggtctctt gcagaagctc ggtacgcgcg gactcttccg
     3901 gcgtgccatg gtcggcgacg ccgccgggca gctcgtaatg aacttggccg atcccgtgtc
     3961 ggtattggcg caccatcacg atccggtcgt ccggcgtcac cgcgatcacg ttgcaccagg
     4021 cttcgtattc gttgacccaa tagcgctcga tctggacgcc gttgggcaac gcgacgcggt
     4081 cttcgcgtac gaccagccac ggcggctctt gatgcaggta gcgtgacgca agcacctgcc
     4141 acggcttcgg atcgtcgctc atcgcggggc gatgctaccg gatgtgcggg accggttttc
     4201 tctccttgac cggcccgggc ggttcgggtt taatcgcgcc tcgcaccaca tggccgtgcc
     4261 catcttcggg ccggccatga cgcttgttgg ggggcttgtg gacaaacctg gggacggtcg
     4321 ggggacgacg cctgtgaaca gcgagaacag ccctgaacag gctgtggacg gctcgttgtc
     4381 ggcggccgcg tctacggtga ggggacgacg atggccattg acctgacccg ggaccgcgag
     4441 gccaaccagg tttcccgcgt tccaccgcac agcctcgacg ccgagcagag cgtgctcggc
     4501 gcgatgatgc tgtccaagac cgcgatctcg gaagtgctcg agatcctcca gcccgacgac
     4561 ttctatcgaa gcgcccacca gcagatcgcg accgcgatgc gcgatctgtt cgcctccggc
     4621 gaagcggtcg atgcgatcac ggtcgtggaa gagatgcggc gcaaaggcac gctggagtcc
     4681 gcgggcggcg cgccctacat ccacacgctg ctcgccggcg tgccgacggc ggccaatgcc
     4741 gcgttctacg cgcagatcat cgtcgagcac tcgacgctgc gacggctcat cgacgcctcg
     4801 acgcgtatcg cgcaagagtg ctacgacatc ccgcccgaca tcgacgaggc aatcaaccgc
     4861 gccggcgagg cgatctacaa ggtcgccgcg cggcgcatcc actcggactt ccgtccgatt
     4921 cgcgagctgc tcgccgagtc gatggagcag ctcgagatgc tcgcctcgcg cgacagcgac
     4981 atcactggcg tatcgaccgg cttccgcgac atcgacgacc gtctgtcggg cctgcagcgg
     5041 cagaacctga tcatcgtcgc cgcgaggccc gccatgggaa agacctcgtt cgtgctcaac
     5101 gtcgcgcaca acgccgcgat gaagtcgaat gtgccggtcg tgatcttttc gctcgagatg
     5161 agccagaacg agatcgtgca gcgtctgatc tcgtccgagt cgcatgtcga ctcgagccgc
     5221 ctgcgcaccg ggcatctgtc cgaggccgac tggacacggg tgtccaacgc cgtcggcaag
     5281 ctcgacaccg ccccgctcta catcgacgac agcgcctcga tttcgatgat ggagatccgt
     5341 gcgaagtgtc agcggctgaa gcagcagcag gatctcgggc tcgtgatcgt ggactacatc
     5401 cagctgatgc aaggatcgcg ccgcaccgaa agccgcgtgc aagaggtcag cgagatctcg
     5461 cgcggcctca agatcctcgc caaggagatg gacgttcctg tcgtcgccgt ctcgcagttg
     5521 tcgcgccagc ccgagcaagg cggcggcgaa cgccgccccc agctgtcgca tctgagggaa
     5581 tgcgtgaccg gcgacacgct tgtctgccta gccgacggac gccgactgcc aatcgaagcg
     5641 cttgtcggca caacgccgat ggtgctcgcc atggacgatc gcggccggat cgtcgaatcc
     5701 gcctcggaca tggtttggaa agtcggccgg cgtccggtct tcagcgtccg gctcgccagc
     5761 ggccgctcca tccgcgccac ggcgaagcac cgcctgttcg gtttcgacgg gtgggtgcga
     5821 gtcgggatgc tggcagccgg agatcgcatc gcgatctctc gcacgctcac gcagccgggg
     5881 aaggtcgagg cgtggccggc ggggcgaccg gccttcaacg ccgtggtatc gggaacttcc
     5941 ggccagcgaa tcttccgacg gcgcatcggc gcgttcggtc cccgcgtcgc tcaggcgacg
     6001 gcgttgaccg agtacttggt ggatcggcct tccaatacga acgtcgacac gatcccggcg
     6061 gctgttttcg atcgcgtgcg gatgcagatg cagcagcgag gaatctccca gcaggcgatg
     6121 gcgctcgctc gaggtcccgc ctacggtgga acgtcgcatt ggcggttcgg tccatcgcgg
     6181 ctcgtcttgg ccgagaatgc cgacatcctc gacgacccag tcctagctga gttggcgtcg
     6241 aacgatctgt tttgggacaa agtcatcgcg gttgagccgg caggcgagga ggatgtatac
     6301 gacttgaccg ttcctgggcc ggcgtcgtgg cttgcagacg ggatcgtgag ccacaactcg
     6361 ggcgcgatcg agcaggacgc ggacgtggtg atgttcgtgt accgcgacga gtactacaac
     6421 aaagaatcgg aagccaaggg cgaagccgag atcatcgtcg caaagcaccg taacgggccc
     6481 gtcggcaccg agaaactggc cttcctgggt cagtacacga agttcgcgga tctggcgcgc
     6541 gagtagcgca ggagtttcgt gggtatctct cctcgtgagg agggacatat gaacatcatc
     6601 tggtggctca tcgtggggtt catcgccggt ctgctggccc gcgccctggt tcccggcgat
     6661 gacagcatgg gcatcgtggg aaccaccgtg ctcggcctcg tcggctcgct cgtgggtgga
     6721 ttcctcggca acctcgtctc cgacagcgac ggcttcagcc cttccgggct gatcggctcg
     6781 atcatcgggg cgatcatcgc actgctgatc tggcgcgcca tggaccggcg cgcgcatgcg
     6841 tagacggctt tgccggcgcg tacactcgcc ggcatggccc aagcagcatc cgaccgggag
     6901 cgcgcgcgtg agatcgtgcg acgactcgcg cgcgcgtacc ggtcgggaca ccctgcccgg
     6961 cccgacacct ctgttgtcgg gtccgtcgtc aagccgccgc tcgacgagct gatcttcacg
     7021 ctgctgtcgc agcacacctc cgacctgaat cgcgatcgcg cctgggtttc gctgcgcgaa
     7081 cggttcccgg cctgggagca ggtcgcgtcg gcccgcacga aatccatcgc ggacgcgatt
     7141 cgcatcggcg gactcgccga ttcgaaggcg cctcgcatca aggccgtctt gcgcgaggtt
     7201 cgcgagcgcg aagggtccta cggcctcgag cgtttgcggc gcatgagcga tgacgacgtg
     7261 cggcactact tgctgtcgct gccgggaatc gggcccaaga cggcggcctg tgtgcttgcc
     7321 ttctcgctcg cgcgtcctgc gcttcccgtt gacacccacg tccatcgtct ggcgatccgg
     7381 ctcggcttcg ccgggcccaa ggcggacgcg gcggccacgc agcagatcct cgagtcgctc
     7441 gttcccgggc gcagccggct cgcgacacac gtgcgtctga tcgcccacgg ccgcatgatc
     7501 tgcaccgcgc ggcggccgaa atgcggcgag tgcgtcttgg ccgatctgtg tccgagcgcg
     7561 gcccggctag catagcggca cgatgcgctc ccggacgcaa gccggatacc tcgagctcgt
     7621 tcttctcggc gtgctctggg gctccatcgg cgtgatcatc gaaccgatcc gcgtgggcgc
     7681 ggcggggatc gcgctcgtgc gaaccgcgac cggatgcctg ctcgccctcg cctacatgct
     7741 gctgcgccga cgagttgcgc gtctgcgcct tcggcgaagt gcctggctgc tgatcgccga
     7801 cggcgtgctg ctggccgttc actggacact gatgttcgag gcgttcaagc gtctgtcggt
     7861 cggcaccgcc atcctgctcg tgttcatcgg cccggtgctc gtcgccgtcg gggcatggcc
     7921 cctgctgcgc gagcgggtcg agacccggac gatcggggcg ctggcggtct cgctcggcgg
     7981 catggcgctg atcgcgattc ccgcgtggga ggtgcaggat cccgccggtg tcgtctatgc
     8041 gttgttgtcg gccgttgcct tcgcgatggt cgtgctcgcc aataagcggc tcaccgcgat
     8101 ctacgagccc gcgacgatcc tggtctggca gctcggcgtc gccgcgctcg cggtcgcccc
     8161 ggtggcgcta accggaacgc tcgacgggtt cggcgaggcg ctgccgcggc tgctgatgct
     8221 cggggcgctg cacaccgccg ccgccggctt catctacttc gccgcggtca gggtggtcaa
     8281 agtacagcac gtcggcgtcc tgacctacct cgagcccgcg accgcgatcg cctacgcgtg
     8341 gatcttcctg tcc
//
LOCUS       DATLFO010000330        11218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_7992, whole genome shotgun sequence.
ACCESSION   DATLFO010000330 DATLFO010000000
VERSION     DATLFO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7992"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="ligD"
                     /locus_tag="VM841_13310"
     CDS             <1..902
                     /gene="ligD"
                     /locus_tag="VM841_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVL91205.1"
                     /translation="PKWRFEPKMDGIRALATVTTDATKLISRNGRDLTVHYPELANLA
                     QYVNAINAIVDGEIVAADEQGRPSFERLQQRMGLTSPRDVDRMRRKIPVTFFAFDLLF
                     FDGRDLTREPLEARREMLAHIVTETERITLTLTLDEHGSRLFEAAKQLGFEGVVAKRL
                     GSVYQPGRRSKEWLKIKAVNRADCVIIGWTTGEGSRSKGFGSLLLGAYRDGEGIWIGQ
                     VGTGFTDAMVAEYRDRLEAIEIERPPFDDPELRGVKGARWVRPELVCDVEYLQMTEAG
                     KLRAPSFKGLRPDKTPEDCTIPD"
     gene            902..1669
                     /locus_tag="VM841_13315"
     CDS             902..1669
                     /locus_tag="VM841_13315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91206.1"
                     /translation="MRTTEMAARVRQGNLDAWRTLAKATGRELVDDRGMLTYFSLAPA
                     PNFNPTIVYEPLADPDASIAARAAAYEARGLAFGFEIPEGLDERCEAAARARGMTLRS
                     TQPAMVLHPVGALPDPDPRVRVVSIDDFDEHLQVAAAGFGDTPDALRVFSPLSMLEAC
                     LAFTAYDEDGTPAATALTCVTGRDAGVFGVATLPAYRRRGLGTAATLAAIRAAAAAGA
                     ELVWLHASEMGEPVYERLGFVTVDLQRIYAPVPSSAS"
     gene            complement(1639..3183)
                     /locus_tag="VM841_13320"
     CDS             complement(1639..3183)
                     /locus_tag="VM841_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91207.1"
                     /translation="MPPRSFEPHRSAPWFDDTPASFPEAAALPRRTGVAVVGAGITGL
                     TTAYLLASQGADVAVIEASAVASGTTGMTTGKVTAQHRLIYARMLAAHGPDRATAYAR
                     AQSAAMTRIEDIARDEGIDCDLHTASSYVYDDDGTRIADLDAELVAATELGLDAAIVE
                     GDIGLPWPVAGALRFDEQLLFHPRRYCFGLAGAVTARGGTIVEGVRATGVREDDDGCE
                     LVTEAGPVRAEAVVLATQIPFVERGLFFARTEPVRSYALAEPRDDAPEGMYISAADPV
                     RSIRPHRSDAGNLLIIGGGGHPTGRGDEGAHEEQLLAWASERFGMNPRWSWSAQDFVA
                     ADGVPFIGPVTGRSRRVFVATGYAKWGMTNGTVAAMLIADEIAGRDNPWRAAFDSTRI
                     DAARSARLVASQGLATMGGLVKDRIDAFAAPGVTRIEAGSGDVVRHDGRTVAVYRDDA
                     GALHGVSARCTHLGCIVRFNDAERTWDCPCHGSRFDPTGRVLDGPATRPLEPVEVQDA
                     EEGTGA"
     gene            complement(3213..4166)
                     /locus_tag="VM841_13325"
     CDS             complement(3213..4166)
                     /locus_tag="VM841_13325"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVL91208.1"
                     /translation="MDLTTNQSRPREDRFRHEALVYDGPDAFAGVVGPFVADALAADE
                     PVLVMTPPDRIDTLRRYLGDDAARVRFEDMTGVGRNPARIIPAWQAFAEEHSGRSVRG
                     VGEPAWAGRSPDELEECRLHESLINLAFRGSDGFWLACPYDGSSLDAPVLEDAHRTHP
                     LAGGTPLISYAGVEDAAGLFAEQLPVPPASAERLDYDNETARSVRRFATEIAQRAGLD
                     AVASANFALTAHELAINSVVHGGGGGTIDAWITPTALIGEVRDRGLIADPLTGRIRPS
                     VDVEGGRGIWLANQLCDLVQLRSSRAGTSVRFHVARSGNTD"
     gene            4265..4642
                     /locus_tag="VM841_13330"
     CDS             4265..4642
                     /locus_tag="VM841_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013128233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL91209.1"
                     /translation="MLAAVDSRDEGSLKGLGSFIRSQRRLANMSLRQLERLTQVSNPY
                     LSQIERGIYRPSAQVLKSIAEALQISAETLYARAGLMEQRPEPAAAGVEQAIAADERL
                     TDDQKEALLGVYRGFVAKTDHPR"
     gene            4736..5050
                     /locus_tag="VM841_13335"
     CDS             4736..5050
                     /locus_tag="VM841_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91210.1"
                     /translation="MAYTAGAEKAADRVLSGVKQAQDMTVNAVSTVTGLVGSFLPEMP
                     SLPFAAKLPRPEAVVKTGFGIAESVLKTNKQYALGLVQAFEPITGKVLPAKQAARKTA
                     SK"
     gene            5228..7729
                     /locus_tag="VM841_13340"
     CDS             5228..7729
                     /locus_tag="VM841_13340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVL91211.1"
                     /translation="MTLRARLSLFFVLIVVVPLAGAIAAVAIFVPRELDQRSVERLRG
                     ASAGAVTLRSERLERAGRDVARIARGLKTAPQEALGARIEAARLESGLDFLVLGSLSS
                     MRSPSFVSAGDPIARIKADVPIGLERASAIIPGRGVLYGGWFTDSSYAKRLAVQTGVS
                     VAFRHGDELLAASSSAAAAAAARAPATGAFDADGQRGVVLPVADSPASLIVMSPAAAG
                     IGEVTGVLGLIGAAGLLMALALGVMIAGAIARPLAELARGANAMAAGDLSVRVRGGGH
                     DEIARVGAAFNTMAASVQSYVNELRDSRDELRRTLERLGATLRSTRGMQEMMTALLEA
                     AVATLGAQGGALYRIAPDGELQLAASQGLEAPTATREPAWEATRSGRPVLLPSLGGDD
                     PLGRTVAAAPLHRGTEIVGAIALTSAGIGPFDERDLATLASFAAQASVAIDNVYLREE
                     AEAMARTDALTGIPNRRTLEAVLTSEVERGRRYRTITSVLMIDLDHFKRINDTCGHLD
                     GDRVLVEAVRRTAAVIRRDVDVLARYGGEEFVLILPETPPAGALVVAEKIREAIEVTP
                     FPVAGGVRVTASIGVAAPPSVAATPMLAVTRTPPRSAGRSTQTDCCTPRTWRSTEPRS
                     RAATESSSPEPLRKEPRHELDPVPRGTRNRHAERGNARDAAGAGRRGRACDRHDPLDG
                     SASGRAVAFGGRTVPRSSRDDQSRRRSPESGRCAGRGRVGRRARVGIRSLRLRSRRGC
                     ASAAAGRRKAGAGPTEAAPAEPRARAARGRRWLGWWRRCASLDDGAGQRLARKRNARW
                     WQSRQRATEPRLSPSGLARWRFAGWRFAGWRFAAS"
     gene            complement(7946..8788)
                     /locus_tag="VM841_13345"
     CDS             complement(7946..8788)
                     /locus_tag="VM841_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91212.1"
                     /translation="MAPRRVSLLLALAFAVSACSSDAVPGGSVAPSQGSGAPAATNPL
                     ASSPASDAPTRAPSVSVLPAPIPPPEDGPLPRPGTYLYAQQGSETFCSTAACSTPRPL
                     PSTMTVTLRYGGRIAGRPDAHRLDITMDIAQGRTVRVKAASSQTDVLVEHIEAEFSES
                     GITRSGSVRPSPAIPLLSFPLREGEEVSRSFRDGDTAGRFTRKVAGRETINAQGRQQP
                     ARKVESTLTTTGDRPASIAITMWIDERSRTFLRLAVEIDVPYEITRYKASFTDTLQSA
                     PASR"
     gene            complement(8992..10128)
                     /locus_tag="VM841_13350"
     CDS             complement(8992..10128)
                     /locus_tag="VM841_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVL91213.1"
                     /translation="MEDLRAAWTLDPAVAFLNHGSFGACPEPVLRAQAEWRARMEREP
                     VLFLDREIDGLLAGARARAARFLRADPDGLVFVPNTTTAVSTVLASIPFADGDEIVTT
                     DQVYGAVRGIIDRICERTGAQARVAPVHLPVSGASAVTGAIAGAITGATRLVIADHVT
                     SPGALVYPIAEIVRHCRSRGVPVLVDAAHGPGMTEVDVDALGADYWCGNFHKWVCAPK
                     GSAALWVTQEHRAGFRPLVTSHPHLETVHQRFDWTGTFDPTAWLAVPAAIDFFDGAGW
                     DRVRARNVEMAERGRAIVAHAIGAPRIAPPDMHAAMTVVPLPAGVAPTRAAASALTRR
                     LYDEHRIEVPVNVAAGQALLRLSAHLYNIESDYERLAAALPALL"
     gene            complement(10136..11071)
                     /locus_tag="VM841_13355"
     CDS             complement(10136..11071)
                     /locus_tag="VM841_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVL91214.1"
                     /translation="MSARDVTPTVAVVPAPIPLAIEAIERAGGMIAAPEEADAIVWTD
                     PAGATALATALPSWPARWIQLPFAGIESFFEAGVIDPSRIWTCAKGIYGPATAEHALA
                     LMLVAARRLHEHTRATEWTGDTDPPMRTLRGTRALVVGTGGIGAALATYLEPLGVEID
                     AISRSGRPAGWARRTATPGDLPALAAECDWLVVAAALTAETRGLIGSAVFDAIRPGAW
                     LTNVARGGLVDTAALVAALEHGRLGGAALDVTDPEPLPPDHPLWRMPDVIITSHTANT
                     RLMALPALAEMVERNVRAFAAGAPLDGLVDPELGY"
BASE COUNT         1685 a         3836 c         4084 g         1613 t
ORIGIN      
        1 acccgaagtg gcgcttcgag ccgaagatgg acggcatccg cgcgctcgcc acggtgacca
       61 cggacgcgac gaagctgatc agccgcaacg gccgcgacct gaccgtgcac tatcccgagc
      121 tcgcgaacct ggcgcagtac gtcaacgcga tcaacgcgat cgtggacggc gagatcgtcg
      181 ccgccgacga gcagggccgg ccgtccttcg agcgcctgca gcagcgcatg ggcctgacca
      241 gcccgcggga cgtggaccgc atgcgcagga agatccccgt caccttcttc gccttcgacc
      301 tgctgttctt cgacggccgg gatctcacgc gcgaaccgct cgaggcgcgc cgcgagatgc
      361 tcgcgcacat cgtgaccgag acggagcgga tcacgctcac gctcacgctc gacgagcacg
      421 gatcgcgctt gttcgaagcg gccaagcagc tcgggttcga gggcgtggtc gccaagcgtc
      481 tcgggtcggt gtatcagccc gggcggcgca gcaaggagtg gctcaagatc aaggcggtca
      541 accgcgccga ttgcgtgatc atcggctgga cgacgggcga aggatcgcgg tcgaaaggct
      601 tcggctcgct gctgctgggc gcctaccgcg acggtgaagg aatctggatc ggccaggtcg
      661 gcaccggctt caccgatgcg atggtcgccg agtaccgcga caggctcgag gcgatcgaaa
      721 tcgaacggcc gccgttcgac gacccggagc tgcgaggcgt caagggggcg cgctgggtcc
      781 gtcccgagct cgtctgcgac gtcgagtatc tgcagatgac cgaggcgggc aagctgcgcg
      841 cgccgtcatt caaaggattg cggcccgaca agacgccgga ggactgtacg attccggact
      901 gatgagaacg accgagatgg ccgcccgcgt gcggcaaggc aacctcgacg cgtggcgcac
      961 gctcgcgaaa gcgaccggcc gcgagttggt cgacgaccgc gggatgctca cgtacttcag
     1021 cctggcgccc gcgcccaact tcaaccccac catcgtctac gagccgctcg ccgatccgga
     1081 tgcgtcgatc gccgcgcgcg cggccgccta cgaggcgcgc ggcctggcat tcgggttcga
     1141 gatacccgag gggcttgacg agcgctgcga ggctgccgcc cgcgcgcgcg gcatgacgct
     1201 gcgatccacg cagccggcga tggtgctcca tccggtcggc gcgctgcccg acccggaccc
     1261 gcgcgtgcgt gtggtatcga tcgacgattt cgacgagcat cttcaggtcg ccgcggccgg
     1321 gttcggcgac accccggatg cgctccgggt cttctcgccg ctgtcgatgc tcgaggcctg
     1381 cctcgcgttc accgcctacg acgaggacgg cacgccggcc gcgaccgcgc tcacgtgcgt
     1441 caccggccgc gacgcgggcg tgttcggcgt ggccacgctg cccgcgtacc gtcggcgggg
     1501 tctgggcacg gccgcgacgc tcgccgcgat ccgcgcggcc gccgcggcgg gcgccgagct
     1561 ggtttggctg catgcgagcg agatgggcga gccggtctac gagcggctcg gcttcgtcac
     1621 ggtcgatctg cagcgaatct acgcgccggt gccctcttcg gcgtcctgaa cctcgaccgg
     1681 ttcgaggggg cgggtggccg gaccgtcgag cacgcggccg gtggggtcga agcgcgagcc
     1741 gtggcagggg cagtcccagg tgcgctcggc gtcgttgaac cggacgatac atcccaggtg
     1801 ggtgcagcgg gcgctcacgc cgtgcagcgc tcccgcgtcg tcgcggtaga cggccaccgt
     1861 ccggccgtcg tggcgaacga cgtcaccgct gcccgcctcg atccgtgtga cgccgggtgc
     1921 ggcgaaggcg tcgatgcggt ccttgacgag cccgcccatg gtggcgaggc cttgcgaggc
     1981 gaccagccgg gcggagcggg cggcgtcgat tcgggtcgag tcgaacgcgg cacgccacgg
     2041 gttgtcgcgg ccggcgatct cgtcggcgat gagcatcgcc gcgaccgtgc cgttggtcat
     2101 gccccacttc gcgtagccgg tcgcgacgaa cacgcgacgg gaccgtccgg tgaccgggcc
     2161 gatgaagggc acgccgtcgg cggccacgaa gtcctgcgcg gaccacgacc accgcgggtt
     2221 catgccgaag cgctcggacg cccacgcaag aagctgctcc tcgtgcgcgc cctcgtcgcc
     2281 gcggcccgtg gggtggccgc cgccaccgat gatgagcagg tttccggcgt cggagcgatg
     2341 gggccggatc gagcggacgg gatcggcggc gctgatgtac atgccctcgg gcgcgtcgtc
     2401 gcgcggctcg gcgagcgcgt aggagcgcac cggctcggta cgcgcgaaga acaggccccg
     2461 ctcgacgaag gggatctgcg ttgcgagcac cacggcttcc gcgcggaccg gccccgcctc
     2521 ggtcacgagt tcgcatccgt cgtcgtcctc gcgcacgccg gttgcgcgga cgccttctac
     2581 gatcgtgccg ccgcgcgccg tcacggcgcc ggccagcccg aagcagtagc ggcgcggatg
     2641 gaacaggagc tgctcgtcga agcgcagcgc cccggccacc ggccagggca gcccgatgtc
     2701 tccctcgacg attgccgcgt cgaggccgag ctcggtcgcg gccacgagct cggcgtcgag
     2761 atcggcgatg cgggtgccgt cgtcgtcgta cacgtacgac gacgcggtgt ggagatcgca
     2821 gtcgatgcct tcgtcgcgcg cgatgtcttc gatccgcgtc atcgccgcgc tctgcgctcg
     2881 ggcgtaggcg gtggcgcggt cggggccgtg cgccgcgagc atccgcgcat agatgaggcg
     2941 atgctgggcg gtgaccttgc cggtcgtcat gccggtcgtg ccggaggcga cggcggaggc
     3001 ttcgatcacc gcgacgtcgg ctccctgcga tgcgaggagg taagccgtgg tcaggccggt
     3061 gatgccggcg ccgaccaccg cgacgccggt gcgtcgcggc agcgcggccg cctcggggaa
     3121 ggatgcgggc gtatcgtcga accagggtgc ggatcggtgc ggctcgaaag accgtggggg
     3181 catatccggg agttaccccg ccgtgcaatc cgtcaatcgg tgttgccgga ccgcgcaacg
     3241 tggaagcgca ccgaggtgcc cgcccggctg ctgcggagtt gcacgagatc gcacagctgg
     3301 ttcgcgagcc agatgcctcg tccgccctcc acgtccacgg agggacggat gcggccggtc
     3361 aacgggtcgg cgatgagccc gcgatcgcgc acctcgccga tgagggccgt gggcgtgatc
     3421 caagcgtcga tcgttccgcc gccgccgccg tggacgacgc tgttgatcgc tagctcgtga
     3481 gcggtgagcg caaagttggc cgatgcgacg gcgtccagcc ccgctcgctg cgcgatttcg
     3541 gtcgcgaagc ggcggaccga tcgcgcggtc tcgttgtcgt agtcgagtcg ttccgccgat
     3601 gcgggcggga cgggaagctg ctctgcgaac aggccggcgg cgtcctcgac gcccgcgtac
     3661 gagatgagtg gcgttccgcc ggcgagcggg tgcgtgcgat gcgcgtcctc gagcacgggg
     3721 gcgtcgagcg aggacccgtc gtaggggcag gcgagccaga agccgtcgct gccgcgaaag
     3781 gcgagattga tcagcgactc gtgcagccgg cattcctcga gctcgtccgg gcttcgtccg
     3841 gcccaggcgg gctcgccgac gccccgtacg ctgcgaccgg agtgctcctc ggcgaaggcc
     3901 tgccacgccg ggatgatccg cgcggggttt cgcccgacac cggtcatgtc ctcgaagcga
     3961 acgcgtgccg catcgtcgcc gaggtagcgt cgaagcgtgt cgatgcggtc cgggggcgtc
     4021 atgacgagca ccggctcgtc ggcggccagc gcatcagcga cgaacggacc gacgacaccg
     4081 gcaaacgcat ccgggccgtc gtagacgagg gcctcgtgcc ggaatcgatc ctcgcgcggc
     4141 cgcgattggt tcgttgtcag gtccacggta gctccgccta acgttacgcc ttttccccca
     4201 ccaggttgtt gctcagcctc gcgggcctta aaccatcctg tgcttggtct cccaagcgag
     4261 tgctatgctc gcggccgtgg actcccggga cgaagggtcg ctcaaggggc tgggctcctt
     4321 catccgaagc cagcgccggc tcgccaacat gtcccttcgg cagctcgagc ggctcacgca
     4381 ggtcagcaac ccctatctgt cacagatcga acggggtatc tatcggccgt ccgcgcaggt
     4441 gctgaagtcg atcgcggagg cgctgcagat ttcggccgag acgctctatg cccgcgcagg
     4501 cctgatggaa cagcgtccgg agccggcggc cgcgggcgtc gagcaggcca tcgccgcgga
     4561 cgagcggctg accgacgatc agaaggaagc gctgctgggt gtctaccggg gcttcgtcgc
     4621 gaagaccgat cacccgcgct gacctgcggt tttccggccg ggcttgacag gaccaagcag
     4681 atgcttggta gacttagcac acttgatcgg acccgcccac aggaggatca cccagatggc
     4741 atacaccgca ggagcagaga aggcagccga ccgtgtcctg tccggcgtca agcaggccca
     4801 ggacatgacc gtgaacgcgg tctcgaccgt gaccggcctc gtcggtagct tccttcccga
     4861 gatgccctcg ctcccgttcg ccgcaaagct tccccggccc gaggccgtcg tgaagaccgg
     4921 cttcggcatc gcagagagcg tgctcaagac caacaagcag tacgccctcg gcctggtcca
     4981 agctttcgag ccgatcaccg gcaaggtgct tccggccaag caggccgccc ggaaaaccgc
     5041 ttccaagtag ttcaacatca tgggaagacc cccggcgtga ccgggggttt tcccattttc
     5101 accctcgaaa tccggcatgg gattccatga aacttccatt aagccgcggg tgaatcgtgc
     5161 cgatacctac caagcgaacc gcacggaacg gttggagccg gacggttggc gtgggaggga
     5221 tagaagcgtg acgcttcgcg ctcggctttc gcttttcttc gtcttgatcg tggtcgtccc
     5281 gcttgccggc gcgatcgcgg ccgtggcgat cttcgttccg cgtgagctcg atcagcgatc
     5341 ggtcgagcgg ctccggggag cgtcggccgg cgcggtaacg ctgcgcagcg aacgcctgga
     5401 gcgggccggc cgcgacgtgg ctcgcatcgc gcgcggcctc aagaccgctc ctcaggaggc
     5461 gctcggggcc cgaatcgaag cggcacggct cgagtcaggc ctcgacttcc tcgttctcgg
     5521 ctcgctttcg agcatgcgct caccgtcgtt cgtgtccgcc ggcgatccga tcgcgcgcat
     5581 caaggcagac gtgcccatcg ggctggagcg tgcctcggcg atcatcccgg gccgcggcgt
     5641 tctgtacggc ggctggttca ccgactcgtc ctatgccaag cgtctcgccg tgcagaccgg
     5701 cgtctcggta gccttccgcc acggggacga gctgctcgcg gcaagctcga gcgcagccgc
     5761 cgcagcggcg gcacgagcgc cggcgaccgg agcgttcgac gccgacggcc agcgcggcgt
     5821 cgtgctgccc gtcgccgatt ctccggcctc gctgatcgtg atgtcgcctg ccgccgcagg
     5881 catcggcgag gtgaccggcg tgctcggcct gatcggcgcg gcggggctgc tgatggcact
     5941 cgcgctcggg gtgatgatcg cgggcgcgat cgcccggccg ctggccgaac tcgcgcgcgg
     6001 cgcgaacgcg atggccgcgg gcgatctatc ggtccgggtc cgcggcggcg ggcacgacga
     6061 gatcgcgcgc gtcggcgccg cgttcaacac catggccgca tccgttcagt cctacgtcaa
     6121 cgagcttcgc gactctcgcg acgagctccg gcgaacgctc gagcgcctgg gcgcgacgct
     6181 tcgctcgacg cgcggcatgc aagagatgat gaccgcgctg ctcgaggcgg cggtcgccac
     6241 gctcggagca cagggaggtg cgctctaccg gatcgcaccc gacggtgagc ttcagctcgc
     6301 cgcatcgcaa gggctcgagg cgccgaccgc cacgcgcgag ccggcgtggg aggcgacgcg
     6361 gtcgggacgc ccggtgctgt tgccgagcct gggcggagac gacccgctcg gccgcaccgt
     6421 tgcggccgcc ccgctccatc gggggaccga gatcgtcggc gcgatcgcgc tcacgagcgc
     6481 cggcatcggt ccgttcgacg agcgcgatct tgcgacgctc gcctcgttcg cggcccaggc
     6541 cagcgtcgcc atcgacaatg tctatctgcg tgaggaagcg gaggcgatgg cgcgaaccga
     6601 cgcgctgacg gggatcccca accgccgcac cctcgaggcc gtgctcacgt ccgaggtcga
     6661 gcgcggccgg cggtaccgca cgatcacgtc cgtgctgatg atcgacctcg atcatttcaa
     6721 gcgcatcaac gacacatgcg ggcatctgga cggcgaccgc gtgctggtcg aggccgtccg
     6781 tcgcaccgcc gccgtgattc gccgcgacgt ggacgtgctc gcgcgctacg gcggagagga
     6841 gttcgttctg atcctgcctg agacgccgcc ggcgggcgcg ctcgtcgtcg cggagaagat
     6901 ccgcgaagca atcgaagtca cgccgttccc cgtcgccggc ggcgtccggg tgaccgcgag
     6961 catcggcgtc gccgccccgc cgagcgtggc ggcgacgccg atgctcgcgg tcacccggac
     7021 gccgccacgc tcggcggggc gctcgacgca gacggactgt tgcacgccgc ggacctggcg
     7081 ctctacggag ccaaggagca gggccgcaac cgagtcgtcg tctccggaac cgctacgcaa
     7141 ggagcctcgt catgagctcg accccgtccc ccgcggcacg cggaaccgtc acgctgagcg
     7201 tgggaacgct cgcgatgctg ctggcgctgg tcgccgtggg cgcgcgtgcg atcgacacga
     7261 ccccctggat ggctccgcat ctggacgggc cgtcgcgttc ggtggccgga ccgtccctcg
     7321 atcgtcccgg gacgatcaaa gccgtcgacg atctcctgaa tccggccgtt gcgcgggtcg
     7381 cgggcgtgtc ggtcggcggg cccgggtcgg gatccgctct cttcgacttc gctcccgtcg
     7441 cggttgcgcg tccgctgccg ccggccggcg gaaagccggc gccggcccaa ccgaagcagc
     7501 cccggccgaa ccccgagccc gtgcagcccg ggggcggcgg tggctcgggt ggtggcggag
     7561 gtgcgcctcc ctcgacgatg gtgccgggca gcggctcgcc aggaagcgga acgcccgatg
     7621 gtggcagtcc cggcagcgag ccaccgagcc gcggctctcc ccctcaggcc tcgcccggtg
     7681 gcggttcgcc gggtggcggt tcgccgggtg gcggttcgcc gcctcctgat gacgatgacg
     7741 atgacgacac gtccgacggc aaggagaaga agagcaagaa ggacgacggc gacaagaaga
     7801 cgggcggcga gaagaagacg ggcgacgaca agaagacggg cgacgacaag aagaagaagg
     7861 atgacggcga aaagaagggc gacggggacg ggagcggatc ggccaagagc ggctcaaagg
     7921 gcaagagcga cgacgatgac gacgactaac gcgacgcggg cgcgctttgg agcgtgtcgg
     7981 tgaacgacgc cttgtagcga gtgatctcgt agggcacatc gatctcgacg gccagccgca
     8041 ggaacgtgcg cgagcgctcg tcgatccaca tcgtgatcgc gatcgaggcg ggccggtcgc
     8101 ccgtcgtggt cagggtcgac tccaccttcc gggccggttg ctgccgccct tgcgcgttga
     8161 tcgtctcgcg gccggcgacc tttcgcgtga agcgccccgc tgtgtctccg tcgcggaacg
     8221 accgtgacac ctcctcgccc tcgcgcagcg ggaatgaaag cagcgggatc gccgggctcg
     8281 ggcgcaccga gccgcttcgg gtaattcccg actcgctgaa ctcggcctcg atgtgctcca
     8341 cgagcacgtc ggtctgtgag gaggcggcct tcacgcgcac cgttcggccc tgggcgatgt
     8401 ccatcgtgat gtcgagccgg tgcgcatccg gccgtcccgc gatccggccg ccgtagcgaa
     8461 gggtcacggt catcgtggac ggcagcggcc gcggggtgct gcatgcggcc gtcgagcaaa
     8521 acgtctcgga gccctgctgc gcgtacaggt aggtgccggg gcgggggagc ggcccatcct
     8581 ccggcggtgg aatcggggcc ggcaagacgg acacggacgg cgcgcgtgtg ggggcatcgc
     8641 tcgccggcga ggaagcgagc gggttcgtcg cggccggcgc gcccgatccc tgtgacggag
     8701 cgaccgagcc gccgggaacc gcatccgaag agcacgccga aacggcgaat gcgagcgcga
     8761 gcagcagcga aaccctccga ggggccatgc gcagatgata gggggtccgc ggccggcgcg
     8821 cgtcagaccg cggggccgga ctcgcgtacc tcttgcacgc tctcgagcgc ctcgcgaagc
     8881 tcgtcgagca ctccttctgc ttggcgccga ccagccgaac gcacctgcgg gcgatgcccg
     8941 cgtccacaag gcgtctccaa gaccttgcgt tccttcattg aacggggcgg gctacaggag
     9001 ggcgggaagc gccgccgcga gtcgctcgta gtcggattcg atgttgtaga ggtgagccga
     9061 caggcggagg agcgcctgcc cggcggcgac gttgacgggc acctcgatgc gatgctcgtc
     9121 atagaggcgg cgagtgaggg ccgatgcggc cgccctggtc ggtgcgacgc ccgcgggaag
     9181 cggcaccacg gtcatcgcgg cgtgcatgtc gggcggggcg atccgcgggg ccccgatcgc
     9241 gtgcgccacg atcgcgcggc cgcgctcggc catctcgacg ttgcgggcgc gaacacgatc
     9301 ccagccggcg ccgtcgaaga agtcgatcgc ggccgggacg gcgagccacg cggtcgggtc
     9361 gaacgtgccg gtccagtcga agcgctgatg cacggtctcc agatgtgggt gggatgtcac
     9421 gagcggacgg aagccggccc gatgctcctg ggtgacccac agagccgccg agcctttcgg
     9481 cgcgcacacc cacttgtgga agttgccgca ccagtagtcg gcgccgaggg catcgacatc
     9541 cacctcggtc atgccggggc cgtgtgcggc gtccaccagg accggcacgc cgcgcgaccg
     9601 gcaatgacgg acgatctcgg cgatcggata gacgagcgct ccgggcgagg tcacgtgatc
     9661 ggcgatcacc aggcgggtcg cgcccgtgat cgcgccggcg attgcaccgg tcacggcgga
     9721 ggcgccggaa acgggaaggt gcaccggcgc gacgcgtgcc tgcgcgccgg tacgctcgca
     9781 gatccggtcg atgattccgc gcacggcgcc gtagacctga tcggtcgtga cgatctcgtc
     9841 gccgtcagcg aacgggatcg acgcgagcac ggtgctcacg gcggtcgtgg tgttcggcac
     9901 gaacacgagc ccgtccgggt ccgcgcgaag gaagcgggcc gcgcgtgcgc gggcgccggc
     9961 cagcagcccg tcgatctcac gatccagaaa caggaccggc tcgcgctcca tgcgcgcgcg
    10021 ccactccgct tgcgcgcgca gtaccggctc ggggcaggcg ccgaaggatc cgtggttgag
    10081 gaacgcgacg gccgggtcca gcgtccaggc ggcgcgaagg tcttccatcg ccgcgctagt
    10141 agcccagctc cggatcgacc aggccgtcga gcggagcgcc ggccgcaaac gcgcgcacgt
    10201 tgcgctcgac catctccgcg agcgccggca gggccatgag gcgggtgttg gccgtgtgcg
    10261 aggtgatgat gacgtccggc atgcgccaaa gaggatgatc cggcggcagc ggctcggggt
    10321 ccgtgacgtc gagcgcggcg ccgccgagcc ggccgtgctc gagcgcggcc acgagcgcgg
    10381 ccgtatcgac gagcccgccg cgtgcgacgt tggtcagcca agcgcccggg cggatcgcat
    10441 cgaagaccgc ggaaccgatc aggccgcggg tttccgcggt gagcgccgcc gcgacgacga
    10501 gccagtcgca ctcggccgcg agcgcgggca gatcgccggg tgtcgcggtt cgccgcgccc
    10561 acccggcggg acggccgctg cgcgagatcg cgtcgatctc cacgccgagc ggctcgaggt
    10621 aggtggccag ggccgccccg atgccgccgg tacccacgac cagtgcgcgc gtgccgcgca
    10681 gggttcgcat cggcgggtcg gtgtcgccgg tccactcggt ggcccgcgtg tgctcgtgca
    10741 gacggcgtgc ggccacgagc atgagcgcga gcgcgtgctc ggcggttgcc gggccgtaga
    10801 tgcccttcgc gcaagtccag atgcggctcg gatcgataac gccggcctcg aagaacgatt
    10861 cgatgcccgc gaacggaagc tggatccatc gtgcgggcca cgacggcagc gccgtggcca
    10921 acgccgtcgc gccggccgga tccgtccata cgatcgcgtc ggcctcttcc ggcgcggcga
    10981 tcatgccacc tgcgcgttcg atcgcctcga tcgccagcgg gatcggtgcc gggacgacgg
    11041 cgaccgtggg agtgacatcg cgagcgctca tgaccgccga tcctatcggg ccggaaaagc
    11101 accgagcctc ccggggggag tgggaggctc ggtgcttttc cggcccgata ggatcggcgg
    11161 tcatgagcgc tcgcgatgtc actcccacgg tcgccgtcgt cccggcaccg atcccgct
//
LOCUS       DATLFO010000331        15426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_146123, whole genome shotgun sequence.
ACCESSION   DATLFO010000331 DATLFO010000000
VERSION     DATLFO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146123"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VM841_13360"
     CDS             <1..472
                     /locus_tag="VM841_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91215.1"
                     /translation="NEASGFGGSYGGYGSYGDPFAGIDDAYDLDDTDEPVRPRSRSPK
                     DPEGKPVKKQKGPPKKTVAARPSARERRLDAEAAAAPKAKARGTTPKPKPRGASGNAG
                     AKGRVTPAPAKRASATPSRPARSNAQKTGRGGSTGAKRKPASGTARPRRSDDLF"
     gene            472..1602
                     /locus_tag="VM841_13365"
     CDS             472..1602
                     /locus_tag="VM841_13365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL91216.1"
                     /translation="MRIVQLTRDFPPAASGIGDHVARTSAALAGGGDEVAVVCSAPAD
                     PWPGIDVRPVIEHWDAAGVAAIVAAVREARPDAIVWHYNPFQIGRRGLAPWAGKLAAA
                     LSRVAPLTVVAHELWYPWGRSGLRGLIWAAGQRRQFRGVAAAARALVATTEARRAHLA
                     KRYPATPVSVVAAGATVDPHPDAPGREAFRSSLGIPGSAFVVAHLGAIGEGRDLRPAL
                     AAIRRLRRDGTDARLLLAGRTGVAPPAGDGIHVTGPRNHALLSAALGSADCYLFAEPT
                     GPVARKTSLLSALAHGLAVVSYTGAAAEAFFRDGETVLLVEPSEERVAGALSRLIADP
                     SLRRRLGDAGAALHAQRFSWAAIAEGIRSAASTDVTHEPHAT"
     gene            1608..3128
                     /locus_tag="VM841_13370"
     CDS             1608..3128
                     /locus_tag="VM841_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91217.1"
                     /translation="MDTQLYPPPALELPERRTMPPWIWSVVMLLGALFLGNQVVSGGP
                     LLRIVAALSMVFIFAVPAVSNPKRAMAMMFGFLPFLGIMRRALVPATGFSALDPLLLV
                     SSAVIFVILLTLIIGGKANLGGTVLSRMVFWLLLIGLLQVFNPAQGNLFVGLTGVMFV
                     LVPMMCFFIGRSVADREMIAKMQRWMIFVGVVSGAYGLYQVLVGFPGFEQAYLARAGF
                     GGLYVGSAIRPFSTFVNPLEYATYLNFAVMTALAYLVYRKGIQRYWIYGALAFMVYAG
                     YLIGSRGFIVLAVIGAIVLIGMRARRAIVGLSLIGLMIGSILLFTSTRSGGEIEADET
                     STAAEQLQERNRVALLDPLDEDRSTLKHHYRGVKDGLIFAFTQQPFGLGTGATGRGSQ
                     KFGDGRSSSTELDLSDATIAFGVVGGTLYLMIIPLAMLQLIRIRVWLPGPMFPAILGM
                     GLVSFGQWLNGGNYAVAPLIWFMMGFADNFYMRARDARARGQNPEDVVDAEAMNPV"
     gene            3128..4252
                     /locus_tag="VM841_13375"
     CDS             3128..4252
                     /locus_tag="VM841_13375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL91218.1"
                     /translation="MRVLLSATDFPPTRGGIQTLLREFCLRTTAEVRVIAPADLGHEA
                     VDSALSAPVRRVRSAGGSGRRGYLPGIALATLRDARAFRPDVYFAGHVLAAPAGVWLR
                     KRHRIPMVIGTYGVELVAPWGRRVAAQVLPHADRTLAVSRFTMDKARELGARDGEVIP
                     VGAPEPREVSAERLAALRERFGLHGSRVLLTVARLEPHKGIDVVTRALASLPSDVRYL
                     VVGSGSSRAHFERVAREADVADRVVFAGGLSDEDLAAAFRIADAFVLASRSLDGGRGG
                     VEGCPVSLLEACAYGLPIVAGRTGGIADAIEDGVTGLLADPENNADIAAALNRALDPS
                     QAKALAEAAEKTAKAERNWSTVVERMQRLLEEAARIRAQR"
     gene            4249..5436
                     /locus_tag="VM841_13380"
     CDS             4249..5436
                     /locus_tag="VM841_13380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91219.1"
                     /translation="MKIAIVHDYLVNRGGAERVVAVLHRIFPDAPIFTGLYAPEATYP
                     EFAAADIRPSKLQRLLRDPEDFRRLAPLMPRTFRKMKIEGFDVVLSSTSGFAHGVKPK
                     NGCHIAYTYTPPRFLWDDGYERANAVSAPLRPLSGLAVRAMRRADRRAAERPHFTVAI
                     STVAAERVRAIYGREATIIHPPVETGMFGIAPNTKDEFLLVSRLLTYKNVDVAIRAFN
                     QLGRTLIIVGDGPARADLEAIAGPTIEFRGVVEDAELVRLYGRCRGTILAASEDFGLT
                     PLEANASGRPVVALRSGGAEETVIDGTTGVFFSPANPLALADAVNRAVHTVFDPVVLR
                     AHAEQFGVEAFEAKIRGFIEQSRRACMTCHRERRAGKVRHLRTVAPGTRDEGTGRAEA
                     KAP"
     gene            5433..6533
                     /locus_tag="VM841_13385"
     CDS             5433..6533
                     /locus_tag="VM841_13385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVL91220.1"
                     /translation="MIRVAFVAPSFGEAEGQGRVNLEIARRLIERGARLDVYTSRLPT
                     GGLPGARVRLAPRPFPIELLNQLLFVAWATLAVARRRYDVVHADGASLLSRADAVAAH
                     MLHSVWREIARGREPGLRGLYRSLSNAINVRLERRAYRRARLVLANSERTADDLVRAI
                     GVARDRIRVIPLGVDAARFHLPSAAERANARARLGAGPDELVAVLVGAVEPRKGVPQA
                     VEAFAGIEGAMLVVVGDTRGGAIKREAAARRARVRFEEWPADPLASYFAADVLLHPAA
                     YEPFGLSVLEGMACGLPAAVSPSAGVAPVAAEASIPVAPDPASIRRVIETLRDDPERR
                     ALMKKRAREIAAARTWDATAEAVLDAWTEVSG"
     gene            6533..9325
                     /locus_tag="VM841_13390"
     CDS             6533..9325
                     /locus_tag="VM841_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HVL91221.1"
                     /translation="MEGGSQIRVLYIDHVTHLSGAEQSLFDLVVGLAGGPIEPVVVLP
                     EDGPLATQLRGAGVLVRKVKMSKRLLETSRATLHTRPLVALIRLVSFVAVGFRLRAII
                     KETNPAVIHTNTLKAHLLAILPAKLSRTPLVWHMRDILPKGWLRRAFNGCARFADRIV
                     VPSRAVAAAFGRNKAVYRKLRLIPNGIRVDTFDDHEDEGLALREEIGAEDADVVIGIV
                     GRIAPWKGQDVFVHAAAMVAERHPEARFAIVGAPMFGDDDFQHELSEMVYQYGLDGQL
                     MFTGWRGAQEAMAALDVVVHASTEPEPFGRTIAEAMAAGLPVVAADGGATREILPPNA
                     GFLVPPGHPEILADALESLLEDDELREQLGENGKAAALRFFDVRRTIGAVAALYAEVV
                     RESVKRAARRRKRARTRKQGRAAARPMPQTRPMNPDRRPPMLRPETPDLSVPQRTARP
                     RRDADPYAAGPARPVRRTARIDDDDDDEAVPPSRAPRPQRAPAAPGRRPVPAMREHAG
                     RAMPRPGAVPHQSRPMPRPGAMPRPGDGRTAAAPPRRIHSPAAFEGGHVIRRPVVLED
                     PRFAADLPAVFESFDRPLEMPPSRRDRRRGPKRHEPAPAAPASFEEDDDDDLEIEGLD
                     AFAEDVTAAPAAPARPAGKTAGPAGQRKKTKESSRATRRRPAPDAPVSADATALVLDP
                     APEPEPVRSEPVPVVPVARAKAPSADKTVTPMSVTVRPKPVYEIVKRIVDVIVATLIL
                     VVGAPLWLLVTLAIKLESRGPALFKGTVWGKDGVPFTYYKFRSMRVEDADDGHRKFIE
                     EYVKRGIHGGVVSEEGKQVFKFVGDSRITRVGRLIRKLSIDEIPQLVNVLKGDMTLVG
                     PRPPLGYEYELYDEWAMQRLVVRPGMTGLQQTYARHSASFTEKVEMDLAYIRDRSLWL
                     DFTILLKTIPAAFKGE"
     gene            9361..10467
                     /locus_tag="VM841_13395"
     CDS             9361..10467
                     /locus_tag="VM841_13395"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVL91222.1"
                     /translation="MKVLVTGGAGFIGLATAEALRDRGDEVRIMDALTQPVHAPDAPG
                     PEGFDFVRGDVRVKDDWIRALDGVDAVCHFAAYQDYLKDFSTFFHVNAAGTAMLYEVI
                     VERSLPVRRVVAASSQAVYGEGAYRCDLDGLVTCEPRPAERLRRGEFEQRCPKCGGPV
                     ELVPTPEEHADPRNSYGISKLAQEKIALALGETYDVPTATMRYSIVHGPGQSPRNAYS
                     GLLRSAALNLLAGVAPVAFEDGKQQRDYVAIGDVVAANLIALDHPDAPGRAYNVGGDM
                     PWTVLEVADALREIAGVDVDTLTPGLYRVGDTRHIVSDVTRLRSLGWNVRADLKQVWR
                     DYWAWLGGLGVDPAIARRAHEDMLARGVLRKAGG"
     gene            10464..11162
                     /locus_tag="VM841_13400"
     CDS             10464..11162
                     /locus_tag="VM841_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVL91223.1"
                     /translation="MKAIVLCAGLGTRLRPLTDTVPKVMVEIGGEPLLFRQLRMLREV
                     GVTQVAMNLHWLGDVIREAVGDGSRFGIEARYGDEPELRGSAGALNGFPGFFDERFFV
                     VYGDVYYEMDLAALADFHAARGAAFTIATAVADDPTRCGVLRIAPDARVEAFVEKPPS
                     APPDAPTNTGVYVCEPRIAALIPPGMSDFGADVIPSLIASGENVYAFSASAIVQDIGT
                     PQGLERARRLASRA"
     gene            11275..12327
                     /locus_tag="VM841_13405"
     CDS             11275..12327
                     /locus_tag="VM841_13405"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91224.1"
                     /translation="MSIVVGRAPARVSLLGGGTDLPAFYERHGGAVVSMAVTLYAYGQ
                     ASPRASDLELVSLDHSTREIIAPQHFARRMRPPILAQEFLTYQKAVAWHFGIERGRLA
                     AASEVPAGVGLASSAAVCVALVMAASRFVGEDMDRRAIAETACRIEMKVLGRSCGKQD
                     QYAAAYGGINALEFFRDGSVEVTPLTLSPETLARLQGRVMLFANGARRNAQTVLAEQA
                     RRSSSDPETVAALHRLKEMAYTARDMLCAGNPDGVGDMLHEAWEAKRHLNSQVHTPEI
                     DRVYALAREAGAIGGKLAGAGLVGTLLLYCPEDRQDEVRRTLASQGWRERTIGIDWGG
                     ASLCANIGGPLVLTET"
     gene            12367..13206
                     /locus_tag="VM841_13410"
     CDS             12367..13206
                     /locus_tag="VM841_13410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL91225.1"
                     /translation="MGALTETKAACPVCGHASARALFVRDGYAIHRCRDCALRFAAPR
                     PSPADLDAVYAAGYFDSKGWVGDPTSDSAYMTRCWGEVAPLLHRRFPERGRLLDVGCA
                     TGAFIAAAARDGWSVAGLEYSDAAAARAREAGLDVRAGILEDEPFAGETFDVAGAWHV
                     VEHLIDPVADLRRIRALTRRGGLLVLETPNDRSIGAKIRGERWAQIRPPEHINFFDRR
                     ALARALGLSAWKIVSARTIYKRDTARRIAGRKALYPVARMAARASEAAGMGGNLRVIA
                     EAL"
     gene            13203..13778
                     /gene="gmhB"
                     /locus_tag="VM841_13415"
     CDS             13203..13778
                     /gene="gmhB"
                     /locus_tag="VM841_13415"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HVL91226.1"
                     /translation="MTSGNGARPAVFLDRDGVICENRDDYVKSWDEFVFVPGAARAVA
                     ELTRAGHRVFIVTNQSAIGRGVVPASEVDKIHDLMTEAIEAEGGRLEEILVCPHAPSD
                     GCGCRKPAPGMLTDAAARHGIDLAGSWLVGDAASDCEAAAAAGCGSVLVLTGRGRAQA
                     RDAKPGYVARDLPEAARWILARLPGEVRETR"
     gene            13784..14860
                     /locus_tag="VM841_13420"
     CDS             13784..14860
                     /locus_tag="VM841_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVL91227.1"
                     /translation="METRNLILKYLSSVEEILRGIDSPTLERVIDVIYDAWTNDRQVF
                     LVGNGGGAATAMHFACDLNKTAMVPGKKRMRARSLSDNVALLTAWANDTHYTNVFGEQ
                     LINFAQPGDLVIGFTASGMSPNIVNAIALANEMGCATIAFVGFDGGTASAIAHHVLHI
                     PSSSYQHIEDVHLLLCHAITSAVQDRARHDPDLGAGLSGDDLNDRLRGIYYVRKALSG
                     EKVLERRLKMIPELAARTIGFDAAVLYRVHRDGLVMMAGGHNIEEVPSFAVDDDVWEA
                     EAIRERRALTAEGGAGGMTSYAVAPVMSDEDGVVAVIAGGYTNGRRVSGSDLQLLQIF
                     AFNLRGALGEGEARLPGGGWWAAK"
     gene            14857..>15426
                     /locus_tag="VM841_13425"
     CDS             14857..>15426
                     /locus_tag="VM841_13425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91228.1"
                     /translation="MTPGRPVRVLLVNPSPIRGGAEEMLDAFLRGFDPARIDASVACL
                     APGDFPDALAAAGHRVHMIDAGRMRQVRRYGQTMRALTRIAREADVVCSWQVKGHYYG
                     TPAARLARTPAIWWDHGIRPKRGEQSAFAGGTIPKALPAGLVLTSSRAAAARHGRAIA
                     IHPGIDVDRFAAARQTARGAMRAHFGVAPD"
BASE COUNT         2164 a         5552 c         5337 g         2373 t
ORIGIN      
        1 caacgaggcc tccggattcg gtggctccta cggcggctac ggcagctacg gcgatccgtt
       61 cgccggcatc gacgacgcct acgacctcga cgacaccgac gagccggtgc ggccgcgctc
      121 acgctcgccg aaggatcccg aaggcaagcc ggtcaagaag cagaaaggtc caccgaagaa
      181 gacggtggcg gcgaggccgt cggcgcgcga acggcgcctc gacgccgaag cggccgccgc
      241 acccaaggcg aaggcacggg gaacgacgcc caagccgaag ccgcggggcg cttcgggcaa
      301 cgcgggcgca aaaggacgcg tcacccctgc gcccgccaag cgcgcgagcg caaccccttc
      361 gcgtccggct cgttccaacg cacagaagac cggacgaggc ggcagcaccg gagcgaagcg
      421 gaagcccgca tccggaaccg cacggcctcg caggagcgac gacctgttct gatgcgaatc
      481 gttcagctga cacgcgactt ccctcccgcc gcgagcggga tcggcgacca cgtcgcccgc
      541 acctcggccg cgctggccgg aggcggcgat gaggtcgccg tcgtgtgctc ggctccggcc
      601 gatccgtggc cgggcatcga cgttcggccc gtgatcgagc actgggacgc cgcgggcgtc
      661 gccgcgatcg tcgccgcggt gcgcgaggca cgtcccgacg cgatcgtgtg gcactacaac
      721 ccgtttcaga tcggccgccg cggcctcgcg ccctgggcgg gaaaactcgc cgcggcactg
      781 tcgcgcgtgg cgccgctcac ggtcgtcgcg cacgagcttt ggtatccgtg gggacggagc
      841 ggtcttcgcg gattgatctg ggcggccggc cagcgccggc agttccgcgg cgtcgccgcc
      901 gcggcgcgcg cgctcgtcgc caccaccgag gcccgccgcg cgcatctggc gaagcgctat
      961 ccggcgacgc cggtatccgt cgtcgcggcc ggagcgaccg tcgaccctca tcccgacgcg
     1021 cccggccgcg aagcattccg ttcctccctg ggcattcccg ggtccgcgtt cgtcgtggcg
     1081 cacctcggcg cgatcggcga aggccgcgat ctccggcccg cgctcgcggc gattcgccgt
     1141 cttcgtcgcg acggcaccga tgcgcggctt ctgctcgccg gccggaccgg cgtggctccg
     1201 cccgccgggg acggtatcca cgtcaccggt ccccgcaacc atgcgcttct ttcagcggcc
     1261 ctcggatcgg ccgactgcta tctgttcgcc gagccgaccg gtcccgtcgc gcgcaagacc
     1321 agcctgctgt ccgcgctcgc gcacggcctc gccgtggtct cctacaccgg cgcggccgcc
     1381 gaggccttct tccgcgacgg ggagacggtc ctgctcgttg aaccttccga ggagcgcgtc
     1441 gccggtgcgc tgtcccgcct gatcgccgac ccgtcgcttc gccggcggct cggggacgcg
     1501 ggcgcggcac ttcatgcgca gcgattctct tgggccgcga tcgcggaagg catccgctcc
     1561 gcggcatcga ccgacgtgac ccacgagcca cacgcgacct agacgatatg gacacgcagc
     1621 tgtatccgcc tccggcgctc gagcttcccg agcggcgaac gatgccgccc tggatctgga
     1681 gcgtcgtcat gctgctgggc gcgctgttcc tgggcaacca ggtcgtgtcc ggtggtccgc
     1741 tgcttcgtat cgtcgccgcc ctgtcgatgg tcttcatctt cgccgtgccg gcggtgtcga
     1801 accccaagcg cgcgatggcg atgatgttcg gcttcctgcc gttcctgggc atcatgcggc
     1861 gcgcgctcgt gccggccacc ggcttcagcg cgctcgaccc gttgctgctg gtctcctcgg
     1921 cggtcatctt cgtgatcctt ctgactttga tcatcggcgg caaggcgaac ctgggcggca
     1981 cggtgctgtc ccgcatggtc ttctggctgc tgctgatcgg gctgctgcag gtcttcaatc
     2041 ccgcgcaggg caacctgttc gtcggcctga ccggcgtcat gttcgtgctc gtgccgatga
     2101 tgtgcttctt catcggacgc agcgtcgccg atcgcgagat gatcgccaag atgcagcgct
     2161 ggatgatctt cgtcggcgtg gtgtcgggcg cctacgggct ctatcaggtg ctggtcggtt
     2221 tccccggctt cgagcaggcg tatcttgctc gcgccggctt cggtggcctc tacgttggct
     2281 cggcgatccg tccgttctcg accttcgtca acccgctcga gtacgccacg tacctgaact
     2341 tcgcggtgat gaccgcgctg gcctatctcg tgtaccgcaa gggtatccag cgctactgga
     2401 tctacggcgc gctggcgttc atggtctacg cgggctatct gatcggatcc cgtgggttca
     2461 tcgtgctcgc cgtcatcggc gcgatcgtgc tgatcggcat gcgcgcccgg cgcgcgatcg
     2521 tggggctgtc gctgatcggg ctgatgatcg gctcgattct gctcttcacc tctacgcgca
     2581 gcggtggcga gatcgaggcc gacgagacgt ccacggccgc cgagcagctg caagagcgaa
     2641 accgcgtcgc cctgctcgac ccgctcgacg aggatcgctc cacgctcaag catcactacc
     2701 gaggggtcaa ggacggcttg atcttcgcct tcacccagca gccgttcggg ctggggacgg
     2761 gcgccaccgg ccgtggctct cagaagttcg gcgacggccg ctcgtcgtcc accgagctgg
     2821 acctgtccga tgcgacgatc gcgttcggcg tcgtcggcgg cacgctgtat ctgatgatca
     2881 ttccgctcgc gatgctgcag ctgatccgga tccgggtctg gcttcccgga ccgatgttcc
     2941 ccgcgatcct gggcatgggg ctggtctcgt tcggacagtg gctcaacggc ggaaactacg
     3001 cggttgcgcc gctgatctgg ttcatgatgg gattcgccga caacttctac atgcgagctc
     3061 gcgatgcgcg cgcccgggga caaaatcccg aggacgtcgt cgacgcagag gcgatgaacc
     3121 cggtctgatg cgcgtccttc tgtccgccac cgactttccg cccacccgag gcggcatcca
     3181 gacgttgctg cgggagttct gcctgcgcac caccgccgag gtgcgcgtga tcgcacccgc
     3241 cgacctcggc cacgaggccg tggattcggc cctttctgct ccggtccgcc gcgtccgctc
     3301 cgcagggggg agcggacggc gcggatacct tccgggcatc gcgctcgcca cgctgcgcga
     3361 cgcgcgtgcc ttccgtcccg atgtgtattt cgcggggcac gtcctggcgg cgccggccgg
     3421 ggtgtggctg cgcaagcgtc accgcatccc gatggtgatc ggcacctacg gcgtcgagct
     3481 ggtcgccccc tggggacgcc gcgtcgccgc gcaggtgctc ccgcacgccg atcgcacgct
     3541 cgcggtcagc cgcttcacga tggacaaggc ccgcgagctc ggcgcccgcg acggcgaggt
     3601 gatcccggtg ggcgcgcccg agccgcgcga ggtttccgcc gagcggctcg ccgcgctccg
     3661 cgagcgcttc ggcctgcacg gatcgcgcgt ccttctgacc gtggcccgcc tggagccgca
     3721 caagggcatc gacgtcgtca cgcgcgcgct tgcgtccctt ccgtccgatg tgcgctatct
     3781 cgtggtggga tcgggctcgt cgcgcgcgca cttcgagcgc gtcgcccgcg aggcggacgt
     3841 ggcggaccgc gtcgttttcg ccggcgggct gtccgatgag gatctcgcgg ccgcattccg
     3901 catcgccgac gccttcgtgc tcgcctcacg ctcgctcgac ggcgggcgcg gcggcgtcga
     3961 aggctgtccg gtatcgctgc tcgaagcttg cgcctacggc ctgccgatcg tcgccggccg
     4021 cacgggcggc atcgccgacg cgatcgagga cggcgtcacc ggcttgctcg ccgatccgga
     4081 gaacaatgcg gacatcgcgg cggccctgaa tcgggcactc gacccatcgc aggcaaaggc
     4141 gctcgcggag gcggcggaaa agacggcaaa ggcggagcgg aattggtcca cggtcgttga
     4201 acgaatgcag cggcttctcg aggaagccgc ccgcattcgg gctcaacgat gaagatagcg
     4261 atcgttcacg attacctggt caaccgcggc ggcgccgaac gggtcgtcgc cgttctgcat
     4321 cgcatctttc ccgacgcgcc gatcttcacg ggcttgtacg cgcccgaggc gacctatccc
     4381 gagttcgccg ccgccgacat ccgcccgtcg aagctgcagc ggctcctgcg ggatcccgaa
     4441 gactttcgcc ggctcgcgcc gctgatgccg cgcaccttcc ggaagatgaa gatcgaaggc
     4501 ttcgacgtgg ttctgtcctc gacgtccggt ttcgcccacg gggtcaagcc gaagaacggt
     4561 tgtcacatcg cctacaccta tacaccgccg cgattcctgt gggacgacgg ctacgagcgc
     4621 gccaacgccg tgtccgcgcc gctgcgtccg ctcagcggcc ttgccgtgcg ggcgatgcgc
     4681 cgcgccgacc gccgcgccgc cgagcgtccg cacttcacgg tcgcgatcag cacggtcgcc
     4741 gccgagcggg tccgcgcgat ctacgggcgg gaggcaacga tcatccaccc gccggtcgag
     4801 accggcatgt tcggcatcgc gcccaacacc aaggacgagt tcctgctcgt gtcgcggctg
     4861 ctgacctata agaacgtgga cgtcgcgatc cgcgccttca accagctcgg ccgcacgctg
     4921 atcatcgtcg gtgacggacc ggcgcgcgcc gatctcgaag cgatcgccgg gcccaccatc
     4981 gagttccgcg gcgtggtcga ggacgccgag ctggtccggc tctacggacg ctgccgcggc
     5041 accatcctcg ccgcgtccga agacttcggc ctcaccccgc tcgaggcgaa tgccagcggc
     5101 cggccggtcg tcgcgctgcg ctccggcggc gccgaggaga ccgtgatcga cggcaccacc
     5161 ggcgtcttct tctcgccggc caacccgctc gcgctcgctg atgccgtcaa ccgcgccgta
     5221 cacacggtct tcgacccggt cgtgctgcgc gcgcatgccg agcagttcgg cgtggaggcc
     5281 ttcgaagcca agattcgcgg gttcatcgag cagtcgcgcc gcgcctgcat gacctgccac
     5341 cgcgagcgcc gcgccggaaa ggtccggcat ctgcgcaccg tcgctcccgg cacgcgcgac
     5401 gagggcaccg gccgggcgga ggccaaggcg ccgtgatccg ggtcgcgttc gtcgccccca
     5461 gcttcggcga agccgaaggg caagggcggg tgaacctgga gatcgcccgc cgcttgatcg
     5521 agcgcggcgc acgcctggac gtctacacga gccgcctgcc caccggcgga ctgcccggcg
     5581 cgcgcgtgcg cctggcgccg cggccgtttc cgatcgagct tctcaatcag cttctgttcg
     5641 tcgcctgggc gacgctggcc gtcgcgcgcc gccgctacga cgtggtgcat gccgacggcg
     5701 cctcgctgct gtcgcgcgcc gatgccgtcg ccgcgcacat gctgcattcg gtgtggcgcg
     5761 agatcgcccg cggacgcgag cccggcctgc gcggcctcta tcgctcgttg tcgaacgcga
     5821 tcaacgtccg cctggagcgc cgcgcctacc gccgcgcgcg tctcgtgctc gccaacagtg
     5881 agcgaaccgc cgacgatctg gtccgagcga tcggcgtggc gcgcgaccgg atccgcgtga
     5941 tccccctcgg cgtggacgcg gctcgcttcc accttccgtc cgctgccgag cgcgcgaacg
     6001 cccgggcgcg gctcggagcc ggacccgacg agctcgttgc ggtgctcgtc ggtgccgtcg
     6061 agccgcgcaa gggtgtgccg caggcggtcg aagcgttcgc cggcatcgaa ggcgccatgc
     6121 tggtcgtggt cggcgacact cgcggcggcg cgatcaagcg cgaggcggcg gcgcgccgcg
     6181 cgcgcgttcg cttcgaggaa tggcctgccg acccgctcgc gtcctatttc gccgcggacg
     6241 tgctgcttca tcctgctgcg tacgagccat tcggattgag cgttctggaa ggcatggcgt
     6301 gcgggctgcc ggccgccgtc tcgccgtccg cgggcgtcgc gcccgtcgcg gccgaggcgt
     6361 cgatcccggt ggcgcccgac cccgcgtcga tccgccgcgt gatcgagacg cttcgtgacg
     6421 atccggaacg tcgcgcgttg atgaagaagc gggcccgcga gatcgcggcc gcacgcacat
     6481 gggacgcgac tgccgaggcg gtcctggatg cttggacgga ggtgagcggc tgatggaggg
     6541 cggatcgcag atccgggtgc tctacatcga ccacgtcacc catctgtccg gtgccgagca
     6601 gagcctgttc gatctggtgg tcggcctcgc cggcggcccg atcgagccgg tggtggtgct
     6661 gcccgaggac gggccgcttg ccacgcagct tcgcggtgcg ggtgtcttgg ttcgcaaggt
     6721 gaagatgtcg aagcggctgc tcgagacctc gcgcgcgacg ctgcacaccc ggccgctggt
     6781 cgcgctgatc cggctggtct cgttcgtcgc ggtgggcttc cggctgcgcg cgatcatcaa
     6841 ggagacgaac ccggcggtca tccacaccaa cacgctcaaa gcgcatctgc tcgcgatcct
     6901 cccggccaag ctgtcgcgca cgccgctggt gtggcacatg cgcgacatcc ttcccaaggg
     6961 ctggctgcgc cgggcgttca acggctgcgc acgcttcgcc gaccgcatcg tcgtgccgtc
     7021 ccgggccgtc gcggcggcgt tcggccgcaa caaagccgtc taccgcaagc tccggctcat
     7081 cccgaacggc attcgcgtgg acaccttcga cgaccacgag gacgaagggc tcgcgctgcg
     7141 cgaggagatc ggcgccgagg acgccgacgt cgtcatcggc atcgtcggcc ggatcgcgcc
     7201 gtggaaaggc caggatgtgt tcgtgcacgc cgcggcgatg gtcgccgagc ggcatcccga
     7261 ggcccgcttc gcgatcgtgg gcgcgcccat gttcggcgac gacgacttcc agcacgagct
     7321 gtccgagatg gtctaccagt acggtctcga cggacagctg atgttcaccg gctggcgcgg
     7381 tgcgcaagag gcgatggcgg cgctcgacgt cgtcgtgcac gcctccaccg agccggagcc
     7441 gttcggccgc accatcgccg aggcgatggc cgccggcttg ccggtcgtgg ccgccgacgg
     7501 cggtgccacg cgcgagatcc ttccgccgaa cgccggattc ctggttccgc cgggccatcc
     7561 cgagatcctg gccgacgcgc tcgagagcct gctcgaggac gacgagctgc gcgagcagct
     7621 cggcgagaac ggcaaggcgg cggcgctgcg gttcttcgac gttcgccgca ccatcggcgc
     7681 ggtggcggcc ctctacgccg aggtcgtgcg cgagtccgtc aagcgtgccg cccgccgccg
     7741 caagcgtgcc cggacccgca agcagggccg cgccgcggcg cgtccgatgc cgcagacgcg
     7801 tccgatgaac ccggaccggc ggccgccgat gcttcgcccg gagacccccg atctttccgt
     7861 gccgcagcgc accgcccgcc cgcgtcgcga cgccgatccg tacgcggccg gtccagcgcg
     7921 tccggtgcgg cgtaccgcgc gcatcgacga cgacgatgac gacgaggccg tcccgccgtc
     7981 gcgcgcgccg cgtccgcagc gtgccccggc cgcacccggc cgccgtccgg ttcccgcgat
     8041 gcgcgagcac gccggccgtg cgatgccgcg tcccggcgcg gtgccgcatc agagccgtcc
     8101 gatgccgcgt cccggcgcga tgccgcgtcc cggcgacggc cggaccgctg ctgcaccgcc
     8161 gcggcgcatc cactcgcctg ccgcgttcga aggcggccac gtgattcgcc gtcctgtcgt
     8221 gctcgaagat cctcgcttcg ctgcagacct gcctgccgta ttcgagtcct tcgaccgtcc
     8281 gctcgagatg ccgccgtcgc gccgcgaccg ccgccgcggg cccaagcgtc acgagcccgc
     8341 gcccgccgcg cccgcctcct tcgaagaaga cgacgacgac gatctcgaga tcgaaggcct
     8401 ggacgcgttc gccgaagacg tcaccgccgc gcccgccgcg cctgcgcggc cggccggcaa
     8461 gacggccggg cccgccgggc aacggaagaa gacgaaggaa tcctcccgcg ccacgcggcg
     8521 tcgcccggcc cccgacgcac cggtctcggc ggatgcgacc gcgctcgtgc tcgatcccgc
     8581 gcccgaaccg gagcccgtcc gcagcgagcc cgtgccggtc gtcccggtgg cgcgcgccaa
     8641 ggctccctcg gccgacaaga ccgtcacgcc gatgtcggtt accgtccggc cgaagccggt
     8701 ctacgagatc gtcaagcgaa tcgtcgatgt gatcgtcgcg acgctgatcc tggtcgtggg
     8761 tgcgccgctg tggctgctgg tgacgctcgc gatcaagctg gagtcgcgcg gccccgcctt
     8821 gttcaaggga accgtgtggg gcaaagacgg cgtgccgttc acctactaca agttccgctc
     8881 gatgcgtgtc gaggacgccg acgacggtca ccgcaagttc atcgaggagt acgtcaagcg
     8941 cggcatccac ggcggcgtgg tctcggaaga ggggaagcag gtcttcaagt tcgtcggcga
     9001 ctcgcgcatc acgcgcgtcg gccgtctgat ccgcaagctc tcgatcgacg agatcccgca
     9061 gctcgtgaac gtcctcaagg gcgacatgac gctggtgggt ccgcgtccgc cgctcggcta
     9121 cgagtacgag ctctacgacg agtgggcgat gcagcggctg gtggtgcggc ccggcatgac
     9181 cggcctgcag cagacctacg cgcgccacag cgcctcgttc accgagaagg tcgagatgga
     9241 cctcgcctac attcgcgacc gctcgctctg gctcgacttc acgatccttc tcaagacgat
     9301 tcccgccgcc ttcaaaggcg agtaatctgc gagccggccg ggtcggcgca aggaggcggc
     9361 gtgaaggttc tggtgacggg cggtgcggga ttcatcgggc tggcgaccgc cgaggcgctg
     9421 cgcgaccgcg gcgacgaggt ccgcatcatg gacgcgctca cccagccggt tcacgcgccc
     9481 gacgcccccg gccctgaggg cttcgacttc gtgcgcggcg acgtgcgcgt caaggacgac
     9541 tggatccgtg cgctcgacgg cgtggacgcc gtctgccact tcgccgccta ccaggactac
     9601 ctgaaggact tctcgacctt cttccacgtc aacgcggccg gcaccgcgat gctctacgag
     9661 gtgatcgtcg agcgctcgct gcccgtgcgg cgcgtcgtcg ccgcctccag ccaggccgtc
     9721 tacggcgagg gtgcgtaccg ctgcgacctc gacggcctgg tgacgtgcga gccgaggccc
     9781 gcggagcgcc tgcggcgcgg cgagttcgag cagcgctgcc cgaagtgcgg cggcccggtc
     9841 gagctggttc ccacgcccga ggagcacgcg gatccgcgca actcctacgg catctccaag
     9901 ctcgcgcagg agaagatcgc gctcgcgctc ggcgagacct acgacgtgcc gacggccacg
     9961 atgcgctact cgatcgtgca cggtcccggg cagtcgccgc gcaacgcgta ctcggggctg
    10021 ctgcgctctg cggcgctcaa cctgctcgcc ggtgtcgcgc cggtcgcctt cgaggacggc
    10081 aagcagcagc gtgactacgt cgcgatcggt gacgtcgtgg ccgccaacct gatcgcgctc
    10141 gatcatccgg atgcgccggg ccgcgcctac aacgtcgggg gggacatgcc gtggacggtg
    10201 ctcgaggtgg ccgacgcctt gcgcgagatc gcgggcgtgg acgtcgacac gctcacgccc
    10261 gggctctatc gcgtcggcga cacccggcac atcgtcagcg acgtgacgcg gctgcgctcg
    10321 ctcggctgga acgtgcgcgc cgacctcaag caggtctggc gcgactactg ggcgtggctc
    10381 ggggggctgg gcgtcgaccc ggccatcgcg cgccgggccc acgaggacat gctcgctcgc
    10441 ggcgtgctgc gcaaggccgg cggatgaagg cgatcgtgct gtgcgccggg ctcggcaccc
    10501 ggctgcgtcc cctgaccgac accgtgccca aggtcatggt cgagatcggt ggcgagccgc
    10561 tgctgttccg ccagctccgc atgcttcgcg aggttggggt cacgcaggtc gcgatgaacc
    10621 tgcactggct cggcgacgtg atccgcgagg ccgtcggcga cggctcccgg ttcggcatcg
    10681 aggcccgcta cggcgacgag cccgagctgc gcggctctgc cggcgcgctc aacggcttcc
    10741 ccgggttctt cgacgaacgc ttcttcgtcg tctacggcga cgtgtactac gagatggacc
    10801 tggcggccct cgcggacttt cacgccgcgc gcggcgccgc cttcacgatc gcgaccgccg
    10861 tcgccgacga cccgactcgc tgcggcgtgc tgcgaatcgc cccggatgcc cgcgtcgagg
    10921 cgttcgtgga gaagcccccg tcggcgccgc ccgacgcccc gaccaacacc ggcgtctacg
    10981 tgtgcgagcc ccggatcgcg gccttgatac ctcccggaat gagcgatttc ggcgccgatg
    11041 tcatcccctc gctcattgcc tccggcgaaa acgtgtatgc cttttcggcc tccgcaatcg
    11101 ttcaagacat aggaacgccg caggggctcg agcgcgcacg ccggctcgcg tcccgcgcct
    11161 gacctgcgga ccggccctcc gtaatggagg ggattcggaa caaccgaacc ggaacggaag
    11221 ggcaggacgg tcgtttcggc gaaacgggac cgcgaggcca aaggagcatc cgcaatgtcg
    11281 atcgtcgtag ggcgcgcgcc ggcgcgcgtg tcgctgctgg gcgggggaac cgacctgccg
    11341 gccttctatg agcgccacgg cggcgccgtg gtcagcatgg cggtcacgct ctacgcctac
    11401 ggacaggcct cgccccgcgc gagcgacctc gagctggtct cgctcgatca ctccacgcgc
    11461 gagatcatcg cgccgcagca cttcgcgcgg cgcatgcgtc cgccgatcct cgcccaagag
    11521 ttcttgacct atcagaaagc cgtcgcatgg catttcggca tcgagcgtgg ccgtctcgcc
    11581 gctgccagcg aggttcccgc cggcgtcggg ctcgcttcct cggccgcggt ctgcgtcgcg
    11641 ctcgtgatgg ccgcgagccg cttcgtcggc gaggacatgg accgccgcgc gatcgccgag
    11701 accgcctgcc ggatcgagat gaaggtgctc ggccgctcat gcggcaagca ggaccagtat
    11761 gcggccgcct acggcggcat caatgcgctc gagttcttcc gcgacggctc ggtcgaggtc
    11821 acgccgctga ccttgtcgcc cgaaacgctc gcgcggctgc agggccgggt catgcttttc
    11881 gccaacggcg cgcggcgcaa cgcgcagacg gtgctcgccg agcaagcccg ccgctcgtcc
    11941 tcggatcccg agacggtggc ggcgctgcac cggctcaaag aaatggccta taccgcgcga
    12001 gacatgcttt gtgcgggcaa tccggacggg gtgggcgaca tgcttcacga ggcatgggaa
    12061 gccaagcgac acctcaactc gcaggtccac acacccgaaa ttgacagggt gtacgcgctc
    12121 gcgcgggaag cgggcgcgat cggaggcaag ctcgccggtg cgggcctggt gggcacgctc
    12181 cttctctatt gccccgagga ccgtcaggac gaggtccggc gcaccctcgc gagccaagga
    12241 tggcgcgagc gcacgatcgg gatcgactgg ggcggcgcaa gtttgtgcgc caatatcggc
    12301 ggtccgctcg ttctaacgga gacataggac cgaaaccgca acgcagcacg aagaaagaag
    12361 gacagagtgg gcgctctgac ggaaacgaaa gcggcatgtc ccgtctgcgg gcatgcatcc
    12421 gcgcgcgccc ttttcgtccg tgacggctac gcgatccatc gctgccgtga ctgcgcgctc
    12481 cgcttcgccg cgccgcgtcc ctcacccgcc gacctcgacg ccgtctacgc ggccggctac
    12541 ttcgactcca aaggctgggt cggcgatccg acgtcggact cggcctacat gacccgctgc
    12601 tggggcgagg tggcaccgct gctgcaccgg cgctttcccg agcgtggccg cctgctcgac
    12661 gtcggatgcg cgacgggcgc gttcatcgcc gccgccgctc gcgacggctg gagcgtggcg
    12721 gggctcgaat acagcgacgc ggccgccgcg cgcgcccgcg aggcgggcct ggacgtgcgg
    12781 gccggcatcc tcgaggacga gcccttcgcg ggcgagacct tcgacgtcgc cggcgcctgg
    12841 cacgtcgtcg agcatctgat cgacccggtc gccgaccttc gccgcatccg cgcgctcacg
    12901 cgccgcggcg ggctgctcgt gctcgagacg cccaacgacc gctcgatcgg cgccaagatc
    12961 cgcggcgagc gctgggcgca gatccggccc cccgagcaca tcaacttctt cgaccgtcgc
    13021 gcgctggcgc gcgcgctcgg cctgtccgca tggaagatcg tgtcggcccg caccatctac
    13081 aaacgcgaca ccgcccggcg cattgccgga cgcaaggcgc tctacccggt cgcccgcatg
    13141 gcggcccgcg cgagcgaggc ggccggcatg ggcggaaacc tgcgcgtgat cgccgaggcc
    13201 ttatgacgag cggcaacggc gcgcggcccg cggtcttcct ggaccgcgac ggtgttatct
    13261 gcgagaaccg cgacgactac gtcaagtcct gggacgagtt cgtattcgtg ccgggtgcgg
    13321 cgcgcgcggt cgccgagctg acccgtgccg ggcaccgcgt gttcatcgtg acgaaccagt
    13381 cggcgatcgg ccgcggcgtg gtaccggcct ccgaggtcga caagatccac gacctgatga
    13441 ccgaggcgat cgaagccgaa ggtggacgcc tcgaggagat tctggtctgc ccgcacgcgc
    13501 cgtcggacgg gtgcggctgc cgcaagccgg ctcccgggat gctcaccgac gccgccgccc
    13561 ggcacggaat cgacctggcc ggctcgtggc tcgtcggcga cgcggcatcg gactgcgaag
    13621 cggccgcggc ggccggatgc ggatcggtgc tggttttgac ggggcgcgga cgcgcccagg
    13681 cgcgcgacgc gaagccggga tacgtcgcgc gggacctgcc ggaggcggcg agatggattc
    13741 tcgcccgtct gccgggcgaa gttcgagaga cgaggtgagt agcgtggaga cgcgcaatct
    13801 gattctgaag tatctgtcgt cggtcgagga aatcttgcgc ggcatcgact cgccgaccct
    13861 cgagcgggtc atcgacgtca tctacgacgc ctggaccaac gaccgccagg tctttctggt
    13921 cggcaacggc ggcggcgccg cgaccgcgat gcacttcgcc tgcgacctga acaagaccgc
    13981 gatggttccc ggcaagaagc gcatgcgcgc gcgatcgctg tccgacaacg tcgcgctgct
    14041 caccgcgtgg gcgaacgaca cgcactatac gaacgtgttc ggcgagcagc tgatcaactt
    14101 cgcgcagccg ggcgatctcg tcatcggctt caccgcgtcc ggcatgagcc cgaacatcgt
    14161 caacgcgatc gcgctcgcca acgagatggg ctgcgccacc atcgccttcg tcggcttcga
    14221 cggcggcacc gccagcgcca tcgcgcatca cgtgctgcac attccgtcga gcagctacca
    14281 gcacatcgag gacgtccacc tgctgctgtg ccacgcgatc acctccgcgg tgcaggaccg
    14341 ggcccggcac gatcccgacc tcggcgcggg cctgtcgggc gacgatctca acgaccggct
    14401 gcgcggcatc tactacgtgc gcaaggcctt gtcgggcgag aaggtgctgg agcggcggct
    14461 caagatgatc ccggagctgg cggcccgaac catcggattt gacgcggcgg tgctgtatcg
    14521 cgtgcaccgg gacgggctcg tgatgatggc cggcggccac aacatcgagg aggttccgtc
    14581 gttcgcggtg gacgacgacg tgtgggaggc cgaggcgatc cgcgagcgcc gcgcgctcac
    14641 cgccgaaggc ggcgccggcg gcatgacctc gtacgcagtc gcgccggtga tgagcgacga
    14701 ggacggcgtc gtcgccgtga tcgccggcgg ctacaccaac ggacggcgcg tcagcggcag
    14761 tgacctgcag ctgcttcaga tcttcgcgtt caatctgcgc ggcgcgctcg gcgagggcga
    14821 agcccggctt ccgggcggcg ggtggtgggc ggccaagtga caccgggccg ccccgttcgc
    14881 gttctcctgg tcaacccgtc gccgatccgc ggcggtgccg aggagatgct cgatgcgttc
    14941 cttcgcgggt tcgatcccgc gcgcatcgac gcgagcgtgg cgtgcctcgc gccgggtgac
    15001 ttccccgacg cgctggcggc cgccgggcat cgcgtgcaca tgatcgacgc gggccggatg
    15061 cgccaggtgc gtcgctacgg gcagacgatg cgggcgctca cgcgaatcgc gcgtgaggcc
    15121 gacgtcgtgt gctcgtggca ggtcaaaggc cactactacg gcacgcccgc cgcgcgcttg
    15181 gcccgcacac cggccatctg gtgggatcac gggatccggc ccaagcgcgg cgagcagtcc
    15241 gcgttcgcgg gcgggacgat ccccaaggcg ctgccggccg gtctggtgct cacgtcctcg
    15301 cgcgcggccg cggcgcggca cgggcgcgcc atcgcgatcc atcccggcat cgacgtcgac
    15361 cggttcgcgg cggcgcgcca gacggcacgc ggcgcgatgc gcgcgcactt cggcgtcgcg
    15421 ccggac
//
LOCUS       DATLFO010000332         7103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_126397, whole genome shotgun sequence.
ACCESSION   DATLFO010000332 DATLFO010000000
VERSION     DATLFO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126397"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VM841_13430"
     CDS             <1..1615
                     /locus_tag="VM841_13430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HVL91229.1"
                     /translation="GATGIEDLDVTFYSDMEAPATSGAATKRSVGGETGIVPAGTTHA
                     IVTLFTGAEADFVYRAWAPVAARPGRTAPLPALPKPGIHASYGKSASPLATALKRKSH
                     VVIALVDTGINPYHSDFRRPELVVHPSQYIEGFPKTAPALGLRLGSKDYVAARNADDG
                     PVWSKVKERSLYWIPGTNVIGAYAHRDYYSTLPVLGSAAIPGVNNMPRPIIDDAGHGT
                     GVASVSAGAKYGSNPEALIVVVEGFGTEAVEWASKQPWIDFISGSYGDPAAVPYGDAA
                     PRIGAANTPAEDVNDEVGEIDSREYEHTGPFVLRDGRTACFSAGNGLSRTGLAYDRYS
                     SLRPTSGPSWIITVGAASPRNDQNYGWHSWPVDVSSYGMHWPAATHTSTDGTWDFSGT
                     SSATPVTCGVFSKALLEARKAMGDTREGIHVTRGTRVPAAGKRGRGLLADGVLTRAEL
                     HEAVLKTALPGAFDPVTFSYEPISTIPDTPVAYTQQGYGVANKASARRAVDVILGRAA
                     MPDRSDVDRWIAAMDVIRDAIWNPANHGG"
     gene            complement(1654..2487)
                     /gene="mutM"
                     /locus_tag="VM841_13435"
     CDS             complement(1654..2487)
                     /gene="mutM"
                     /locus_tag="VM841_13435"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HVL91230.1"
                     /translation="MPELPEVEIVRKDLERDVVGRKIKDVTVKLPRIIRRHKNRKEFT
                     DRLTGRKIAKIERRGKYILLGLDSEDVLVMHLGMSGHVERATGRKAPDKHTHVIIKFV
                     TGGELRFVDMRQFGELFVSARDDLDGVKELQHIAIDPLEDSFTWQYFSGLLVGRQVKL
                     KTLLMDQKFISGIGNIYSDEILWAAGLRHDRLSDSLTSQEVRRLYRSIQEVLQEGIRH
                     RGVTLEDETYKDLYGETGSMQDHLKAYGREGEPCRRCRTPIVRERWSNRSTYFCPQCQ
                     V"
     gene            complement(2488..3237)
                     /gene="rnc"
                     /locus_tag="VM841_13440"
     CDS             complement(2488..3237)
                     /gene="rnc"
                     /locus_tag="VM841_13440"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVL91231.1"
                     /translation="MRLFRRRTTKPGPTPLEQRLRVRFRNRDLMALALTHRSYAYEQG
                     GLPTNERLEFLGDAVLGIVVTDALYDNFPDLAEGDLAKMRAALVNMTVLADVAKEIEL
                     GTSVMLGRGEEMTGGRDKPSILSDTLEAVFGAIYLDMGIEAASRVVLDLFLPRILGRV
                     EGGTVQDYKTSLQEMAAARVGSLPEYRIVESGPDHAKRFKATVRLAGQEFGSGEGRSK
                     KEAEQAAARIAVERIKADDAGLPPGRTGARS"
     gene            complement(3237..3419)
                     /gene="rpmF"
                     /locus_tag="VM841_13445"
     CDS             complement(3237..3419)
                     /gene="rpmF"
                     /locus_tag="VM841_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HVL91232.1"
                     /translation="MPVPKRKKPKTRTRSRKSQWFGSASAPSYADCPQCKKPKLPHRA
                     CPNCGTYAGRQVLPVE"
     gene            complement(3425..3970)
                     /locus_tag="VM841_13450"
     CDS             complement(3425..3970)
                     /locus_tag="VM841_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HVL91233.1"
                     /translation="MTRSGVALLSENPLKIEVGDLLRRPGGSKSLDLAAHVPGLEVGL
                     GRVAGDGALGLSLLLEALAEGVLVTGQVTGTFVLECSRCLTEFEAGFTVDLHEVYTYP
                     DQPEIEEGFVVWDDEIDLVPAIRDAVLLAVPDNPLHDEACKGLCATCGQDLNVADCGH
                     AHQAVDVRWEPLRRLRDEIEE"
     gene            complement(4080..4619)
                     /locus_tag="VM841_13455"
     CDS             complement(4080..4619)
                     /locus_tag="VM841_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91234.1"
                     /translation="MAKGEYVTDEAIPISVGAVNVLEDLIAKIEDVVRTARSMPLSSS
                     AMVPRDELLDMISSLKAALPEEVGRARSLIRDAEAVLQRAREEADKIVERAKADRERA
                     IAKTEIVQAASREAEKLVSSAEAHAKRIRAEAEQYVEGKLANFEVALQKTLKAVERGR
                     ARIAGRLADAEEITADEDE"
     gene            complement(4606..5094)
                     /gene="coaD"
                     /locus_tag="VM841_13460"
     CDS             complement(4606..5094)
                     /gene="coaD"
                     /locus_tag="VM841_13460"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HVL91235.1"
                     /translation="MSDRIAVYPGTFDPITNGHLDIIERARAQFGSVVVAVSQNPSKN
                     PLFPLEERIAIAKETTAGMDGVNVDSFEGLLVDYVRARGASALVKGLRAVTDFDYELQ
                     MAQMNYRLAGVETMFLVANPEYSFLSSSLVKEVARFGGDVEGMVPPEVLRRLKDRFHG
                     ER"
     gene            complement(5091..5702)
                     /gene="rsmD"
                     /locus_tag="VM841_13465"
     CDS             complement(5091..5702)
                     /gene="rsmD"
                     /locus_tag="VM841_13465"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HVL91236.1"
                     /translation="MSLRVISGEAKGRRLKTPPGLETRPTSDRVRQTLFDILGDVSGD
                     HVLDLFAGSGALGIEALSRGASRAIFVESDPVAVRVVRENLAVCGFEGRAKVVRADAE
                     DYVQRRSGDSPCDLAFLDPPYERGLAFVARLMGSLAARDRIAAGGRVVAESAAGDVEL
                     PEAFRLLRVKVFGRTQLTIAVREPEMNVVREGTPEATEPDGQS"
     gene            complement(5699..>7103)
                     /locus_tag="VM841_13470"
     CDS             complement(5699..>7103)
                     /locus_tag="VM841_13470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVL91237.1"
                     /translation="PYDPTGAQRRAVAEILGDMARPVPMHRLLQGEVGSGKTVVAVAA
                     AVAAAANGKQTALMAPTEVLAEQHYLSITRMLEAIAALAPADDSPGQLTLEKPGFRVE
                     LLTGSVTPKERERSNEGARSGRAHLLIGTHALIQGDVEFAALGLAIIDEQHRFGVHQR
                     LALRAKGAGAAQPDQLIMTATPIPRTLAMTLYGDLDVSTLDEMPPGRTPVRTVVAGPE
                     DRRRAEARIRVEVEQGRQAFIVCPLVEKSASLEAKAAETEYERIRHEVFPDLRVGLIH
                     GRLKPADKEAVMQRMRAGEIDILVATTVIEVGVDIPNASVMMIEDADRFGLSQLHQLR
                     GRIGRGTHPSTCILLTSLEPGDEDREDARRRLDAMAGTNDGFRLAEVDMEIRGEGQIF
                     GRGKVDPGAGEGAPAQAGATDLRFASLLRDADLLADARSEAFDLVGSDPDLRRHPLLL
                     SEVRRRFADRLDWLFAG"
BASE COUNT         1127 a         2463 c         2377 g         1136 t
ORIGIN      
        1 gggcgcgacc gggatcgagg atctcgacgt caccttctat tccgacatgg aggcgcccgc
       61 caccagcggc gcggccacca agcgcagcgt cggcggcgag accggcatcg ttccggcggg
      121 cacgacccac gcgatcgtga cgctgttcac cggcgccgaa gccgacttcg tgtaccgggc
      181 ctgggcgccg gtcgcagccc ggccgggtcg caccgccccc cttccggctc tgccgaagcc
      241 cggcatccac gcctcgtacg gaaagagcgc ctcgccgctc gcgaccgcgc tcaagcgcaa
      301 gtcgcacgtg gtgatcgcgc tcgtggacac cggcatcaac ccctatcact ccgatttccg
      361 ccggcccgag ctcgtcgtgc acccgagcca gtacatcgaa ggattcccca agaccgcgcc
      421 cgcgctcggc ctgcggctcg gctcgaagga ctacgtggcc gcgcgcaacg ccgacgacgg
      481 gcctgtgtgg agcaaggtca aagaacgctc gctgtactgg atccccggaa ccaacgtcat
      541 cggcgcgtat gcgcaccgtg actactactc gaccttgccg gtgctgggca gcgcggccat
      601 tccgggggtg aacaacatgc cgcgtccgat catcgacgac gccggccacg gcaccggcgt
      661 ggccagcgtg tcggccggcg cgaaatacgg atccaacccc gaggcattga tcgtcgtggt
      721 cgaagggttc ggcaccgagg cggtcgaatg ggcatcgaaa cagccctgga tcgacttcat
      781 ctccggctcc tacggagatc cggccgcggt cccctacggg gacgccgctc cccggatcgg
      841 cgccgcgaac accccggccg aggatgtgaa cgacgaggtc ggcgagatcg actcgcgcga
      901 gtacgagcac acgggtccgt tcgtcctgcg cgacgggcgc accgcatgct tcagcgccgg
      961 caacggcctg agccgcaccg gcctcgccta cgaccggtac tcctcgctgc ggcccacatc
     1021 gggaccgtcg tggatcatca cggtcggagc cgcgagcccg cgcaacgacc agaactacgg
     1081 atggcattcc tggccggtgg acgtgtcctc ctacggcatg cattggccgg cagcgacgca
     1141 cacttcaacg gacggcacgt gggacttcag cggaacctcg agcgccacac cggtcacctg
     1201 cggcgtgttc tcgaaggccc tgctggaggc gcgcaaggcc atgggcgaca cgcgcgaggg
     1261 catccacgtg acgcgcggca cgcgcgtgcc ggccgcggga aagcgcggcc gggggcttct
     1321 cgccgacggc gtgctgaccc gcgcggagct tcacgaggcg gtgctcaaga ccgcactgcc
     1381 cggcgcgttc gatccggtga cgttctccta cgagccgatc tcgacgattc cggatacgcc
     1441 ggtcgcgtac acgcagcagg gctacggcgt ggccaacaag gcttcggcgc ggcgcgcggt
     1501 ggacgtgatc ctgggccgcg cggcgatgcc cgaccgaagc gacgtggacc gctggatcgc
     1561 ggcgatggac gtgatcaggg acgccatctg gaaccccgcc aaccacgggg ggtagggatt
     1621 gcaccaacct tgacggcgcg cttcggcgcg ccgtcagact tggcactgcg gacagaagta
     1681 cgtgctgcgg ttggaccagc gctcgcgcac gatcggcgtc cggcatcggc ggcacggctc
     1741 gccctcgcgc ccgtaggcct tgaggtgatc ctgcatcgag ccggtctcgc cgtacagatc
     1801 cttgtaggtc tcgtcctcga gcgtcacgcc gcgatgacgg atgccttcct gaaggacctc
     1861 ttggatcgac cggtacaggc ggcgcacctc ttgcgaggtc agcgagtccg agagccggtc
     1921 gtgccgaagg cccgccgccc acaggatctc gtcgctgtag atgttgccga tgccgctgat
     1981 gaacttctga tccatcagaa gcgtcttgag cttcacctgc cggccgacga gcaggcccga
     2041 gaagtactgc cacgtgaacg agtcctcgag cggatcgatc gcgatgtgct ggagctcttt
     2101 gacgccgtcg aggtcgtcgc gcgcgctgac gaacagctca ccgaactggc gcatgtccac
     2161 gaagcgaagc tcgccccctg tgacgaactt gatgatcacg tgtgtgtgct tgtcgggtgc
     2221 cttgcgtccg gtggcgcgct cgacatggcc gctcatgccc aggtgcatca cgagcacgtc
     2281 ctcggagtca agaccgagca agatgtactt gccgcggcgc tcgatcttgg cgatcttgcg
     2341 gccggtgagc cggtcggtga actccttgcg gttcttatga cgcctgatga tccgcggaag
     2401 cttgaccgtg acgtccttga tttttcggcc cacgacgtcg cgttcgagat ctttgcgaac
     2461 gatctcgact tcgggaagct cgggcatcta gcttcgcgct cccgtgcggc cgggcggcaa
     2521 acccgcgtcg tcggccttga tgcgctcgac ggcgatgcga gcggctgcct gctcggcttc
     2581 tttcttggac cggccctcgc cggatccgaa ttcctggccg gcgaggcgga ccgtagcctt
     2641 gaagcgcttc gcgtggtcgg gtcccgactc gacgatgcgg tattcgggca aggaccccac
     2701 gcgcgcggcg gccatctctt gcaagctcgt cttgtagtcc tgcacggtgc cgccttcgac
     2761 gcggccgagg atgcggggca ggaacaggtc cagcacgacg cgcgacgcgg cctcgatgcc
     2821 catgtccagg tagatcgctc cgaagacggc ctcgagggtg tccgaaagga tcgagggctt
     2881 gtcgcggccg ccggtcatct cctcgccgcg gcccagcatc acggaggttc ccaactcgat
     2941 ctccttggcg acgtcggcaa gcacggtcat gttgaccagc gccgcgcgca tcttggcgag
     3001 atctccctcg gccaggtccg ggaagttgtc gtagagcgcg tccgtgacca cgatgcccag
     3061 cacggcgtca ccgagaaact ccaggcgctc gttcgtcggc aatcctccct gctcgtaggc
     3121 ataagaacga tgcgtcaagg ccaaggccat caggtctcgg ttccgaaagc ggacacgcag
     3181 ccgctgctcc agcggcgtag ggccgggctt cgtcgtgcgc cggcgaaaca gtcgcatcac
     3241 tcgaccggca acacctgacg cccggcgtag gtcccgcaat tgggacacgc acggtgggga
     3301 agcttcggct ttttgcattg ggggcagtcg gcgtaggagg gcgccgacgc cgagccgaac
     3361 cactgcgact tgcgcgagcg ggtccgggtc ttcggcttct ttcgcttcgg tacgggcatt
     3421 gcagctactc ctctatctcg tcgcgtagcc gccggagcgg ctcccatcga acatcgaccg
     3481 cctgatgggc atgcccgcag tcggcaacgt tcaggtcctg tccgcacgtc gcgcacagcc
     3541 ccttgcacgc ctcgtcgtgc aacgggttat ccggcacggc gagcaggacc gcatcccgga
     3601 tcgccggaac caggtcgatc tcgtcgtccc aaacgacgaa accctcctcg atctccggct
     3661 ggtccgggta ggtgtagacc tcgtgcaggt ccaccgtgaa accggcctcg aactcggtga
     3721 ggcaccgaga gcattccagc acgaaggtgc cggtgacctg cccggtgacc aggacgcctt
     3781 ctgccagcgc ttccagcagc agcgacaggc cgagcgcccc gtcgccggcg acccgcccga
     3841 gcccgacctc caggccgggg acgtgcgccg ccaggtccag gctcttcgag ccacctggac
     3901 ggcggaggag gtcccctacc tcgatcttca gggggttttc ggacagcaag gcaacaccgg
     3961 acctggtcat gggcgccaca gaatagcaca gcgggtttac accggttccg acctaggttt
     4021 gcactgccca aatcgacaaa taccggcacg ccggaagacc gccggaacga ccggagaggt
     4081 cactcgtcct cgtcggcggt gatctcctcg gcgtcggcca gccggccggc gatgcgcgcg
     4141 cggccccgct cgacagcctt gagcgtcttt tgcagggcaa cctcgaagtt ggcgagcttc
     4201 ccctcgacgt actgctcggc ctcggctcga atgcgcttag cgtgggcttc ggccgacgag
     4261 accagcttct cggcctcgcg ggaggcggcc tgaacgatct cggtcttggc gatggcgcgc
     4321 tcacgatcgg ccttggcccg ctcgacgatc ttgtccgcct cctcgcgcgc ccgctgcagg
     4381 acggcctcgg catcgcgaat cagggagcgg gcccgcccga cctcctccgg cagggccgcc
     4441 ttgagcgagg agatcatgtc gaggagctcg tcgcgcggaa ccatggcgct cgacgacagc
     4501 ggcatcgacc gggcggtgcg gaccacgtcc tcgatcttgg cgatcaggtc ttccagaacg
     4561 ttgacggcac cgaccgagat cgggatcgct tcgtcggtca cgtactcacc tttcgccatg
     4621 gaaccgatcc ttcagccgac gcaggacctc gggaggaacc atgccctcga cgtcgccgcc
     4681 gaaacgggcg acctccttga ccaaggacga ggacaggaac gagtactcgg ggttcgcgac
     4741 gaggaacatc gtctcgacgc cggccagccg gtagttcatc tgcgccatct gcagctcgta
     4801 gtcgaagtcg gtgacggctc gcagcccttt gaccaaagcc gacgcgccgc gggcgcggac
     4861 gtagtccacg agcagcccct cgaacgagtc cacgttcacc ccgtccatcc cggcggtggt
     4921 ctccttggcg atggcgatgc gctcctcgag cgggaacagg gggttcttcg acgggttctg
     4981 cgacacggcc accacgacgg acccgaactg ggcgcgtgcc cgctcgatga tgtcgagatg
     5041 cccgttcgtg atgggatcga acgttcccgg gtagaccgcg attcgatcgc tcatgactgt
     5101 ccgtccggct cggtggcctc cggagtcccc tctctgacga cgttcatctc tggttctctg
     5161 acggcgatgg tgagctgcgt ccggccgaag accttcaccc gcagcagtcg gaacgcctcc
     5221 gggagctcga cgtcgcctgc ggccgattcc gcgacgacgc gcccgcctgc ggcgatccgg
     5281 tctcgtgccg cgagggatcc catcaggcga gcgacaaatg ctaagccgcg ctcgtacggg
     5341 gggtcaagaa aagccaggtc gcagggggaa tcgccgcttc tgcgctgcac gtagtcctcg
     5401 gcgtcggccc gaacgacctt ggcccggccc tcgaatccgc acacggcgag gttctcgcga
     5461 acgacccgca ccgccacagg gtcggactcg acgaagatcg cgcgggaggc gccccgcgac
     5521 agggcctcga tacccaatgc gccggagcca gcgaacagat cgagcacatg gtcgccggac
     5581 acatcgccca agatgtcgaa cagcgtctgg cgaacccggt cggaggtcgg gcgcgtctcg
     5641 aggcctggcg gggttttgag gcgccgtccc ttcgcctcgc ccgagatcac gcgcaggctc
     5701 atccggcgaa cagccagtcc aggcggtcgg cgaagcggcg gcgcacctcg gacagcagca
     5761 acgggtggcg ccgcaggtcg ggatcggagc ccaccagatc gaaggcctcg ctccgggcgt
     5821 cggccagcag atcggcgtcc cggagcaggc tcgcgaaccg caggtcggtc gctccggctt
     5881 gggcgggcgc gccttcgccg gcgccgggat cgaccttgcc gcggccgaag atctgccctt
     5941 ccccgcggat ctccatgtcc acctccgcca ggcggaaccc gtcgttggtg ccggccatgg
     6001 cgtcgagacg gcgtcgcgcg tcctcgcgat cctcgtcgcc gggctcgagc gaggtaagca
     6061 ggatgcaagt ggagggatgc gtgccgcggc cgatccggcc gcgaagctga tgcaactgcg
     6121 acagcccgaa gcggtcggcg tcctcgatca tcatcaccga ggcgttcggg atgtccacac
     6181 cgacctcgat cacggtggtc gcaaccagga tgtcgatctc gccggcgcgc atgcgctgca
     6241 taacggcctc cttgtcggcc ggcttgagcc gcccgtggat caggccgacc cgaagatccg
     6301 ggaagacctc gtggcggatg cgctcgtatt cggtctcggc ggctttcgcc tccagcgatg
     6361 ccgacttctc gaccagcggg cacacgatga aggcctgccg gccttgctcg acctcgacgc
     6421 gaatgcgcgc ctcggcgcgg cggcggtcct cgggcccggc gacgacggtg cgcacgggcg
     6481 tgcggccggg cggcatctcg tcgagcgtgc tcacgtccag gtcgccgtag agcgtcatcg
     6541 cgagcgttcg cgggatcggc gtcgcggtca tgatgagctg gtcgggctgg gccgcgccgg
     6601 cgcctttcgc ccgcagcgcg agccgctgat gcacaccgaa gcgatgctgc tcgtcgatga
     6661 tcgcgagccc gagcgccgcg aactccacgt ccccttggat gagcgcgtgc gtgccgatga
     6721 gcaggtgcgc gcgcccgctg cgcgcgcctt cgttcgaacg ctcgcgctcc ttgggcgtga
     6781 ccgaaccggt cagaagctcg acgcggaagc cgggcttttc cagcgtgagc tggccgggcg
     6841 agtcgtcggc gggcgcgaga gcggcgatcg cctccagcat gcgcgtgatc gacaggtagt
     6901 gctgctcggc caggacctcg gtgggcgcca tcagcgccgt ctgctttccg ttcgcggcgg
     6961 ccgcgaccgc ggcggcgacc gcgacgacgg tctttccgct gccgacctcg ccttgcagca
     7021 gacggtgcat cgggaccggc ctggccatgt cgccgaggat ctcggccacg gcccgccgct
     7081 gcgcgcccgt cgggtcgtag ggc
//
LOCUS       DATLFO010000333         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_98127, whole genome shotgun sequence.
ACCESSION   DATLFO010000333 DATLFO010000000
VERSION     DATLFO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98127"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VM841_13475"
     CDS             complement(<1..700)
                     /locus_tag="VM841_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL91238.1"
                     /translation="MPSLKLPASRRRLPLLGASHDGDFRAPHSHFWCDTADDVRLAGT
                     ALGDPASETAVVLAHGFMGYRRKMKNRLLAESLAARFRVFAFDMRGHGESGGACAGGA
                     IEHLDVEAAVEAARRRGHGRVITVGWSLGGIAVVRHAAEHGGVDGVVAISTPASWMSE
                     SKAVRRTTWLFTSSLGRELARRVMRTRIDFKPGMPDPPVEVIRKVEAPVLIVHGDNDH
                     FFGPDAARALFEAAN"
     gene            842..1513
                     /locus_tag="VM841_13480"
     CDS             842..1513
                     /locus_tag="VM841_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012835612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL91239.1"
                     /translation="MADILIVTDRTDGVTSLAAGLEDGGHRVGIAGFAEARGSSPDPD
                     VLIVDSVKDLSLGRDTCRHARSASSRLRIAAILDPDTLDAIGPDWETDTFLVDGCAGA
                     EALARIRLVLGSPVTESSSVTRVGDLVIDPETYQVRLRGRPLDLTYKEFQLLSFLAQR
                     PGRVYSRSMLLQEVWGYDFFGGTRTVDVHVRRLRSKLGPEHESMIATVRNVGYKIEGP
                     PRARG"
     gene            1523..>2577
                     /locus_tag="VM841_13485"
     CDS             1523..>2577
                     /locus_tag="VM841_13485"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVL91240.1"
                     /translation="MIDTEYVLRTVEERDIRFIRLWFTDVLGFLKSFAITPAELEAAF
                     DEGMGFDGSAIEGFARVHESDMVARPDPSTFQILPWRPEQAGVARMFCDVLTPDGEPS
                     PVDPRWVLKRALSRAQEMGFTFYVGPELEYFYFRDQESCEPLDLGGYFDLTPLDVSSD
                     LRKRTVFYLEQVGVPVESVHHEVAPSQHEIVLRDSDALAMADAVMTARLAVKEVAAEA
                     GVYATFMPKPIQGTWGSGMHTHMALYEGERNAFHDPDAPDNLSKVARAFVAGVLNHAR
                     ELTAVCNQWVNSYKRLVPGFEAPTSICWAHQNRSAMLRVPNRRADVRVEIRTIDPSCN
                     PYLAFAAILRAGLNGIAT"
BASE COUNT          412 a          881 c          853 g          431 t
ORIGIN      
        1 cattcgcggc ctcgaagagc gcacgggcag cgtcgggccc gaagaagtgg tcgttgtctc
       61 cgtgcacgat cagcacaggc gcttcgactt tgcggatcac ctcgacgggc ggatcgggca
      121 tgccgggctt gaagtcgatg cgggtgcgca tcacgcggcg tgccagttcg cgcccgagcg
      181 agctggtgaa cagccacgtt gtgcgccgaa cggcttttga ttccgacatc cacgacgctg
      241 gcgtcgatat cgcgacgaca ccgtcgactc cgccgtgctc cgcggcgtgc cgcaccacgg
      301 cgattcctcc gagcgaccat ccgaccgtga tcacgcgccc gtgaccgcga cggcgcgccg
      361 cttcgacggc tgcttcgaca tccagatgct cgatcgcgcc accggcgcac gcgccgccgg
      421 actcgccgtg gccgcgcatg tcgaaggcga agacgcggaa acgcgccgcg agcgactcgg
      481 ccagcagccg gtttttcatc ttgcgtcgat agcccatgaa cccgtgagcg agaacgaccg
      541 cggtctcgct cgccggatcg ccgagggccg ttccggcgag ccggacgtcg tcggccgtgt
      601 cgcaccaaaa gtgcgaatgg ggcgcacgaa agtctccgtc gtgggaagcg cccagcaatg
      661 gaaggcggcg gcgagaggcc gggagcttca gggaaggcat tggttctcct tgtggggtcg
      721 cggtgtctct gctactttgc ggtctacgga gccgccgcgc aatgtgaacg ccggcaccgc
      781 gccctcaggc acagttgccg cggtctgggg gtttttcgtt ttctcgcaaa ggaccgggtt
      841 cgtggccgac atactgatcg tgaccgacag gaccgacggc gtgacatcgc tcgccgccgg
      901 cctcgaggac ggcggccatc gcgtgggcat cgccggcttc gccgaagcac gcggctcgtc
      961 gcccgacccc gacgttttga tcgtggactc ggtcaaagat ctgtccctcg gccgcgatac
     1021 ctgccggcac gctcgcagcg cgtcgtcacg gctccggatt gccgcgatcc tggaccccga
     1081 cacgctcgac gcgatcggtc ccgattggga aaccgacacg ttcctggtgg acggatgcgc
     1141 cggcgccgag gcgctcgcac gcatccggct ggttctcggc tcgccagtga cggagtcgtc
     1201 gagcgtcacg cgggtcggcg atttggtgat cgacccggag acctatcagg tgcgcctgcg
     1261 cggccgcccg ctcgacctga cctacaaaga gtttcagctt ctttcgttcc tggctcagcg
     1321 gccgggtcgc gtctactcgc gaagcatgct gctgcaagag gtatggggat acgacttctt
     1381 cggcggcacc cgcaccgttg acgttcacgt gcggcggctg cgatccaagc tcggccccga
     1441 gcacgagtcg atgatcgcga cggtgcgcaa cgtgggctac aagattgaag ggccgccacg
     1501 cgctcgcgga tagaatgaag ccatgatcga caccgagtac gtgctgcgca cggtcgaaga
     1561 gcgcgacatc cgattcatcc gcttgtggtt caccgacgta ctcggtttct tgaagtcgtt
     1621 cgcgatcacg cccgccgagc tcgaggccgc cttcgacgag ggcatgggct tcgacggcag
     1681 cgcgatcgaa ggattcgcgc gcgtgcacga gtccgacatg gtcgcgcggc cggacccgtc
     1741 cacgtttcag atccttccgt ggcgtcccga gcaggcaggg gtcgcgcgca tgttctgcga
     1801 cgtgctcacg cccgacggcg agccgagccc ggtcgatccg cgctgggtgc tcaagcgcgc
     1861 gctgtcccgc gcgcaagaga tgggcttcac gttctacgtc ggtccggagc tcgaatactt
     1921 ctacttccgc gaccaggagt cgtgcgagcc tctcgacctc ggcggctact tcgacctgac
     1981 gccgctcgac gtgtcgtccg accttcgcaa gcgcacggtc ttctatctcg agcaggtggg
     2041 cgtcccggtc gaatccgtcc atcacgaggt cgcgcccagc cagcacgaga tcgtgttgcg
     2101 cgactcggac gcgctcgcga tggccgatgc ggtgatgacc gcgcggctgg cggtcaaaga
     2161 ggtcgcggcc gaggcaggcg tgtacgcgac gttcatgccg aagccgatcc aaggcacgtg
     2221 gggcagcggc atgcacacgc acatggcgct ctacgaaggc gagcgcaatg ccttccacga
     2281 cccggacgcg cccgacaacc tgtccaaggt cgcgcgcgcg ttcgtcgccg gcgtgctcaa
     2341 ccacgcgcgc gagctcaccg ccgtctgcaa ccagtgggtg aactcctaca agcggctcgt
     2401 cccggggttc gaggcgccga cctcgatctg ctgggcgcac cagaaccgaa gcgcgatgct
     2461 gcgcgtcccc aaccgccgcg ccgacgtgcg cgtcgagatc cggaccatcg atccgtcctg
     2521 caacccgtac ctggccttcg cggcgatcct gcgcgccggc ctcaacggca tcgccac
//
LOCUS       DATLFO010000334         6759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_8084, whole genome shotgun sequence.
ACCESSION   DATLFO010000334 DATLFO010000000
VERSION     DATLFO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8084"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VM841_13490"
     CDS             complement(<1..504)
                     /locus_tag="VM841_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91241.1"
                     /translation="MKRAIASSILVAAAAGLLAPPARATSAGTTQRASVPVSGVDSAP
                     GRSSFNPAVSFDGRFVAFDSDSPALIEHDRNDHADVFRFDRRSGTVERVSVSSGGSEG
                     NAGSYDPAMSADGRFVAFASSASNLIPGDRNNTFDVFVRDLARGSTRLASVDPTGKAF
                     TSGSFDPA"
     gene            595..2040
                     /locus_tag="VM841_13495"
     CDS             595..2040
                     /locus_tag="VM841_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL91242.1"
                     /translation="MRPIRTIWFLLFAFAAAPLPLMGAVEPVVVAIVDSGLNGAHEAF
                     DYRGPESGDDQVVAWWDFTRPGVPGPSDRWDARAEPSDETGHGTVVAGLVAGRVPGEW
                     IPAEGFEIAVAKVCDLGGCRGDIQGAIRWAVDVAGADVVVVSLGAGVPIPESVAGVHE
                     AVAYARQHGALTVVSVGNGLANRNVGPGAGSITPFGSSADALVVGNDGGTGLQETWDP
                     EVVADPFTDRAPGAWCVRCSTSARGSSIANARVGSVAAAALALARQAGLNPSPDEVET
                     LLKRAARDTERAPSIEGYGVLDRTGFDELARHAVAGTTPPRPDGDVNSIYVEHVAGGL
                     RSVWSRSLNGDATLLKCHEDPDARSGVIGGGVHPGFGRAERWDVGPDHGVVRVTIEFS
                     SATQDLDLYAVEPAESGWRVVARADSRGSGSDSVMVSTARPVSFLVVGRVVLTEQPYR
                     IAVVGPGGDMTAHSAGGLVCVSTTPRVVHVS"
     gene            complement(2045..3541)
                     /locus_tag="VM841_13500"
     CDS             complement(2045..3541)
                     /locus_tag="VM841_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL91243.1"
                     /translation="MDNSDDLRLLLASRYPLIIATEREETRLLAVVRRAATALRTPVY
                     IWSCTRGLGLDGGAPMYDTIDPTKALAAIEQISGPAVFVLVDAHHPLADPLVVRRVKE
                     LSQHPEPGRTIVATTPQPFMPPEIEGIALAWALKPPSRAELHQLAVRTIQDLTARGIP
                     VALDAAGAEALIDALRGLSLGEAERLIQKAAFHDGKIDDGDVTFLRGEKAALLESSGS
                     LELIEADHGSLADVGGLDALKAWLAMRGRAFEPAAKQFGLTPPRGVLITGVPGCGKSM
                     IAKTLARSWGMPLVLLDASRLYGPYVGETEQRTREALARVESMAPVVLWVDEIEKGFP
                     TGDGNDGGVSKRMLGTFLRWMQDRDDGVFIVATANQVTQLPPELLRKGRFDEIFFVDL
                     PEPAERADIVRLHLTRRGRDPQHFDVDRLAALCEGFSGAEIEAAIVAALYRAYAAGRD
                     LHTGDVAAEVEATSPLSVTRAEEVAAIRAWSQGRAVPASGASAAARTA"
     gene            complement(3612..6044)
                     /locus_tag="VM841_13505"
     CDS             complement(3612..6044)
                     /locus_tag="VM841_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91244.1"
                     /translation="MLGAVLAVASIAVAGPSPSPAASTPAQLVAEAEWILSMRVTAGP
                     DAGLIRSNAGHDCIPYFSNIAASGLAAATRATGDVRYVNAAWDWLDWYASRMNAQGFV
                     TNYRWDGARWASTGTFDSTDGYAGTFLVAVRDAYAASGDATRLSALWPAVGKAVNAIL
                     ATKDSDWLTYARPGWPHKYLMDNVEAYEGFRAVEELAGDAHPDARLRVLAGMWAANMP
                     AALEIFWNPAQGGYDAAISSTGVRYPFQWSSLYPIVTAQAWMLRTGLVTPERAASIAQ
                     RIEQTHPGWDASGQQSGSAWWPEIVEGHRWGGMSPRAASGLARMLSTVAAAGRAWPYH
                     VGNAGRLLTQMAGEPQTRVLAMPDAFRRPGTQTIRFTGVPSIGDAGLASFECRIDAGS
                     WAPCASPIEVADASDGRRTVHLRAYDDGGARDLVPAVVSWTVDSLPPSVTLTGTPAEP
                     GNAAPVFSFAGSDDAVPPDLLTFECATNAGSYAPCASPATLSVAGTGTHTARIVARDP
                     AGNVSAPATHAWLADGTLPETTLLTGPAGIVASGEATFTFDGSDDSGPVAFECRLSGA
                     PFAACSSPVSYTLGEGTWTFDVRAVDHVGNRDSSPASRTWYVDRTPPATVLLEAPQPW
                     SRVTTARFRFVGLDNVANSTMITFRCSLDGAPFSLCTTPMNVSGLAPGPHTFEVYALD
                     GAGLADPTPERVEWTVDPVAPAGTIATPDGWVLTAAAPLLTRSVSGQAGDDASGVGTV
                     IATFYPADPPDGPPVVSGAAVTCADESRTSCTWTVTAPAARGRYRVLVTVADRAGNEA
                     ATRPADIAVLVV"
     gene            complement(6164..>6759)
                     /locus_tag="VM841_13510"
     CDS             complement(6164..>6759)
                     /locus_tag="VM841_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91245.1"
                     /translation="LGTLDGRGSLAYGYPYGGYGTSAPLFDHDSGPGRLIASRARGAA
                     ASDFRGVYTFPLAMMTNAGQRAQLAQEVLAFLGAARASSTRPPSIRFERFMHVVSGTD
                     KAVAVGASGAGLSRVELWYRPHGLTEWTVQALKPADAGLYRGTIPGSAIGSNGIDYFV
                     RAMAGSTPIAVSAGGAAMPNVASGSYGAPAAGTVPCP"
BASE COUNT         1002 a         2316 c         2458 g          983 t
ORIGIN      
        1 ggcgggatcg aagctgccgc tggtgaacgc ctttccggtg gggtccaccg aggcgagccg
       61 tgtcgaaccg cgcgcgaggt cgcgaacgaa gacgtcgaag gtgttgttgc ggtcccccgg
      121 aatcaagttc gaggccgaag acgcgaaggc gacgaaccgt ccgtccgccg acatcgcagg
      181 atcgtagctt ccggcattgc cttcggatcc gccggacgag accgaaaccc gttcgaccgt
      241 gccgctgcgc cggtcgaatc gaaagacgtc ggcgtgatcg ttgcggtcgt gctcgatgag
      301 cgcgggtgag tccgagtcga aggcgacgaa tcgcccgtcg aagctgaccg ccgggttgaa
      361 gctggaccgg ccgggggcag aatcgactcc cgagacgggg acggaggcgc gttgcgtggt
      421 tccggctgaa gtggcccggg ccgggggcgc gagcaacccg gccgcggcgg cgaccagaat
      481 cgacgaggcg atcgcgcgct tcatgctgat cctttccgga cgatctattt cgccggtcgt
      541 ggtcgcgaca cctgccagtg gaggagaatc cacccggccc gcgaaaagta tcgcatgcgc
      601 ccgattcgaa cgatttggtt cctgctcttc gcattcgccg cggcgccgct gcccctgatg
      661 ggcgcggtcg agcccgtggt cgtggcgatc gtcgactccg ggctcaacgg agcgcacgaa
      721 gcatttgact atcgaggccc cgaaagcggc gacgaccagg tggtcgcgtg gtgggacttc
      781 accagacccg gcgtgccggg gccatcggac cgctgggatg cgcgcgccga accatcggat
      841 gagaccgggc acggaacggt cgtcgcgggc ttggtcgcgg gacgagttcc gggcgaatgg
      901 atccctgccg aaggattcga aattgcggtt gcgaaggtgt gtgacctcgg cgggtgccgg
      961 ggcgacatcc agggcgccat ccgctgggcc gtcgacgtcg ccggtgcgga tgtcgtcgtc
     1021 gtgagcctcg gtgccggcgt cccgattccg gaaagcgtcg cgggcgtcca cgaggccgtg
     1081 gcgtatgcga ggcaacacgg tgcgctgacc gtcgtgtcgg tgggcaatgg tttggcgaac
     1141 cggaacgtcg ggccgggcgc cggttcgatc acgccgttcg gatcctcggc cgacgccctg
     1201 gtcgttggca acgacggcgg cacggggctg caggagacgt gggatccaga agtcgtcgcc
     1261 gatccgttca cggatcgcgc gccgggtgcc tggtgcgtac ggtgctcgac gtcggcacgg
     1321 ggatccagca tcgccaacgc acgcgtcggt tcggtcgcgg ccgccgcgct ggccctcgca
     1381 cggcaggccg ggctgaatcc gtcgcctgac gaggtcgaga cgcttctcaa gcgcgcggct
     1441 cgcgacacgg agcgagcacc atcgatcgaa gggtacggag tcttggatcg cacgggattc
     1501 gacgagcttg cgcggcacgc ggtggccggg acgacgcccc cccggcccga cggggacgtg
     1561 aactccatct acgtcgagca cgtggccggg ggtctgcgat ccgtctggtc acgatcgttg
     1621 aacggcgatg ccacgctgct gaaatgccac gaggatccgg acgcgcggtc gggagtgatc
     1681 ggcgggggtg tgcacccagg cttcgggcgc gccgaacggt gggatgtcgg gccggatcac
     1741 ggcgtggttc gagtcaccat cgagttcagc tcggccacgc aggacctgga tctgtacgcg
     1801 gtcgagccgg cggagtccgg ctggcgcgtg gtcgctcgcg cggattcgcg cggaagcgga
     1861 tccgattcgg tcatggtctc gaccgcgcgc ccggtgagct ttctcgtcgt aggtcgtgtc
     1921 gttctgaccg agcaaccgta ccggatcgcg gtcgtgggcc ccggcggaga catgactgcg
     1981 cactcggccg gcggccttgt gtgcgtctcg accacgccac gcgtggttca cgtgtcctag
     2041 tcggttaggc cgtgcgtgcg gccgccgaag cgcccgatgc cggcaccgcg cgcccctgcg
     2101 accacgcacg gatcgctgcg acctcttcgg cgcgcgtgac cgacagcggc gaggtcgcct
     2161 caacctcggc ggcgacgtcg ccggtgtgca ggtcgcgacc ggcggcgtag gcacggtaga
     2221 gcgccgcgac gatcgcggct tcgatctcgg cgccgctgaa tccctcgcac agcgcggcga
     2281 gacgatcgac gtcgaagtgc tgcggatcgc ggccgcggcg cgtcaggtgc agccgcacga
     2341 tgtcggcgcg ctcggcgggc tcgggcaggt ccacgaagaa gatctcgtcg aagcgtccct
     2401 tgcgaagaag ctcgggcggc agctgcgtga cctggttggc ggtcgcgacg atgaacacgc
     2461 cgtcgtcgcg gtcctgcatc cagcgcagga acgtgccgag catgcgtttg ctgaccccgc
     2521 cgtcgttgcc gtcaccggtc gggaagcctt tctcgatctc atccacccac agcacgaccg
     2581 gcgccatcga ctcgacgcgc gccagcgctt cgcgcgtgcg ctgttcggtc tcgcccacgt
     2641 acggcccgta cagccgcgac gcgtccagca gcaccagcgg cattccccag cttcgcgcga
     2701 gcgtcttggc gatcatcgac ttgccgcagc ccggcacgcc ggtgatcagc acgccgcgcg
     2761 gcggcgtgag cccgaactgt ttcgcggccg gctcgaacgc gcgtccgcgc atcgcgagcc
     2821 atgccttgag cgcatccagg ccgccgacgt ccgccagcga gccgtggtcg gcttcgatca
     2881 gctcgagcga gccgctcgac tccagcagcg cggccttctc gccgcgcaga aacgtcacgt
     2941 cgccgtcgtc gatcttgccg tcgtggaagg cggccttttg gatcagccgt tccgcttcgc
     3001 cgagcgacag cccgcgcagc gcgtcgatca gcgcctcggc gcccgcggcg tccagcgcga
     3061 cgggaatacc gcgggcggtg aggtcttgga tggtgcgcac ggcgagctga tgcagctcgg
     3121 cgcgtgacgg cggcttgagc gcccacgcga gcgcgatgcc ttcgatctcg ggcggcatga
     3181 acggctgcgg cgtggtcgcc acgatcgtcc ggccgggctc ggggtgctgc gaaagctcct
     3241 tgacgcgccg caccacgagc ggatcggcga gcggatggtg cgcgtccacg agcacgaaca
     3301 cggcaggccc agagatctgc tcgatcgcgg ccagcgcctt cgtgggatcg atcgtgtcgt
     3361 acatcggcgc gccgccgtcg aggcccaggc ctcgcgtgca cgaccagatg tagaccggtg
     3421 tgcgcagcgc ggtcgcggcg cggcgcacga ccgcgagcag ccgcgtctct tcgcgttcgg
     3481 tcgcgatgat cagcggatag cgcgacgcga gcagcaggcg cagatcgtcc gagttgtcca
     3541 tgccacttcg ggtatcggcc ggtctctcgg cgcccttaac cgcaaaaggc ggggccgtcc
     3601 ggacagcccg gttagacgac caggacggcg atgtcggcgg gccgggtcgc cgcctcgttg
     3661 ccggcccggt cggcgacggt gacgagcacg cggtaccggc cgcgcgcggc cggcgcggtc
     3721 accgtccagg tgcaggaggt gcgcgactcg tcggcacacg tgaccgcggc gccgctcacg
     3781 accggcgggc cgtccggcgg atcggcgggg tagaacgtcg cgatcaccgt gcccacgccc
     3841 gaggcgtcgt cgcccgcctg gcccgagacg ctgcgggtca gcagcggcgc ggcggcggtg
     3901 agcacccatc cgtcgggggt ggcgatcgtg ccggccggtg cgaccggatc cacggtccac
     3961 tcgacgcgct ccggggtcgg gtccgcgagg cccgccccgt ccagcgcgta gacctcgaag
     4021 gtatgcgggc cgggcgcgag ccccgacacg ttcatcgggg tcgtgcacag cgagaacggt
     4081 gcgccgtcca gcgagcagcg gaaggtgatc atcgtcgagt tcgcgacgtt gtcgaggccc
     4141 acgaaccgga accgcgcggt cgtcacgcgc gaccagggtt gcggcgcctc cagcagcacc
     4201 gtcgcgggcg gggttcggtc cacgtaccag gtgcggctcg ccggcgacga gtcgcggttg
     4261 ccgacgtgat cgacggcccg gacgtcgaag gtccaggtcc cttcgccgag cgtgtacgag
     4321 accgggctcg agcacgccgc gaacggcgca cccgacaacc ggcactcgaa tgcgaccggt
     4381 cccgagtcgt cggacccgtc gaacgtaaac gtcgcctcgc cgcttgccac gatgccggcg
     4441 gggccggtga ggagcgtggt ctcgggaagc gtgccgtccg ccagccacgc gtgcgttgcg
     4501 ggggcgctga cgttcccggc cggatcgcgg gccacgatcc gagcggtatg cgttccggtg
     4561 ccggcgaccg acagagtggc gggcgatgcg cacggcgcgt acgaaccggc gttcgtcgcg
     4621 cactcgaacg tcagcagatc cggagggacg gcgtcgtcgg aaccggcgaa cgaaaagacc
     4681 ggcgcggcgt ttccgggttc ggcgggcgtg ccggtcagcg tcaccgacgg gggcaacgag
     4741 tcgacggtcc acgacaccac cgccgggacg agatcccggg cgccgccgtc gtcgtaggcg
     4801 cgcaagtgca cggtgcgccg gccgtcggat gcgtccgcga cctcgatcgg tgaggcgcag
     4861 ggcgcccacg agcccgcgtc gatacggcat tcgaacgacg cgagccccgc gtccccgatc
     4921 gagggaacgc cggtgaagcg aatggtctgc gttcccggcc ggcgaaacgc atccggcatc
     4981 gcaagcacgc gcgtctgggg ctcgccggcc atctgcgtga gcagccgtcc ggcgttgccg
     5041 acgtgatacg gccacgcacg gccggcggcg gcgaccgtcg agagcatgcg cgcgagcccc
     5101 gacgccgccc gcggtgacat cccgccccat cggtggcctt ccacgatttc gggccaccag
     5161 gccgatccgc tctgctgtcc ggacgcgtcc cagccgggat gcgtctgctc gatgcgttgc
     5221 gcgatcgacg ccgcgcgttc gggcgtgacc aggccggtcc gcagcatcca ggcctgcgcg
     5281 gtcacgatcg gatacagcga gctccactgg aacgggtacc gaacgccggt gctgctgatc
     5341 gccgcgtcgt atccgccttg cgccggattc cagaagatct cgagcgccgc cggcatgttc
     5401 gcggcccaca ttccggcgag cacccgcagc cgcgcgtccg gatgcgcgtc gcccgcgagc
     5461 tcctcgaccg cgcggaatcc ttcgtaggcc tcgacgttgt ccatcagata cttgtgtggc
     5521 cagcccggcc gcgcgtacgt cagccagtcc gagtccttcg tggccaggat cgcgttcacc
     5581 gctttgccga ccgcgggcca cagcgcgctc aggcgcgtcg cgtcgccgct ggccgcatag
     5641 gcgtcgcgaa cggccacgag gaacgtgccc gcgtagccgt cggtcgagtc gaacgtgccc
     5701 gtcgaggccc accgcgcgcc gtcccaccgg tagttggtca cgaagccttg cgcgttcatg
     5761 cgcgaggcgt accagtcgag ccagtcccac gcggcgttca cgtagcgcac gtccccggtc
     5821 gcgcgggttg cggccgcaag acccgaggcc gcgatgttcg agaagtaggg gatgcagtcg
     5881 tgacccgcat tcgagcggat cagcccggca tcgggcccgg cagtgactcg catcgacagg
     5941 atccactcgg cctcggccac gagctgcgca ggcgtcgagg cggccgggga aggcgacggc
     6001 cccgcgaccg cgatcgaggc gacggccaag accgccccca gcatggctct ccgaatggtc
     6061 cggccctccc gatcacccga ggtgtccgat taatcccgta ctgagaagta tgccacgtta
     6121 agtacggata ttcccacctg gaggattgtt ccggcggggc gccttaaggg caggggaccg
     6181 tgccggccgc gggagcgccg taggagccgc tcgccacgtt cggcatcgcc gcgccgccgg
     6241 cggagaccgc aatcggtgtg ctcccggcca tcgcccgtac gaagtagtcg atgccgttcg
     6301 agccgatcgc cgaacccggg atggtgccgc ggtagagccc cgcgtccgcc ggcttgaggg
     6361 cctgcacggt ccactccgtc agaccgtgcg gccgatacca cagctcgacc cgggacaagc
     6421 ccgcgccgga tgcgcccacg gccacggcct tgtccgtgcc cgacacgacg tgcatgaagc
     6481 gttcgaagcg gatcgagggc gggcgtgtcg aagaggcgcg ggccgctccc aagaacgcaa
     6541 gcacctcctg cgcgagctgc gcgcgttgcc cggcgttggt catcatcgcg agcggaaagg
     6601 tgtacacgcc gcggaagtcg ctcgcggccg cgcctcgagc gcgcgacgcg atcaggcggc
     6661 ccggcccgga gtcgtgatcg aacagcgggg cggatgtgcc gtagccgccg tacgggtagc
     6721 cgtaggcgag cgatccgcgc ccgtcgagcg taccgagat
//
LOCUS       DATLFO010000335         7006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_146254, whole genome shotgun sequence.
ACCESSION   DATLFO010000335 DATLFO010000000
VERSION     DATLFO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146254"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="thiC"
                     /locus_tag="VM841_13515"
     CDS             <1..954
                     /gene="thiC"
                     /locus_tag="VM841_13515"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVL91246.1"
                     /translation="VEDLTAEDLLAAIEHQAMQGVDYMTVHAGVRLRHLPLVQHRITG
                     IVSRGGGLLARWMVHHGRENPLYERFDDVLEIARRHDVSLSLGDGLRPGCLADASDRA
                     QFAELDTLGELTRRAWDAEVQVMVEGPGHVPMDQLEANVRRQQEVCGEAPFYTLGPLV
                     TDVAPGYDHITSAIGAAMIGSYGTALLCYVTPKEHLGLPDRDDVRTGVISYKIAAHAA
                     DLAKGHPRAQEWDDAISKARFEFRWEDQFELALDPETARSFHDETLPAEPAKKAHFCS
                     MRITQDVRAYAKEHGIGEDRVLEVGLKEKAEEFRKTGAVYR"
     gene            987..1607
                     /locus_tag="VM841_13520"
     CDS             987..1607
                     /locus_tag="VM841_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HVL91247.1"
                     /translation="MLLLLIRHALTARTGVKLSGWTPGIHLSKVGRAQAESLAERLDA
                     VPLTAIYASPLERTAETAAPVARAKRLKVRTREDVGEVRYGDIEGRSLRALNKTKIWT
                     QLRAWPSDVRFPGGESLRETQARAVTAIEDIRRKHPDGVVAVFSHGDWIRLAMAHHMG
                     IHIDLYRRLSVDPVSVNVLQFYDMGVQVRRLNEIGTLGDLAPGAKR"
     gene            1604..2137
                     /locus_tag="VM841_13525"
     CDS             1604..2137
                     /locus_tag="VM841_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HVL91248.1"
                     /translation="MSESFDLDPVERITAGAIGDPGQRTFFVQAASSFEQVTLLAEKE
                     QVRILAQAILQLLQQLPGDDEEDVSPSLLALADPLAPAWRAGEMAIEYDEDADRVEIV
                     IREAVPDEDDEAAVPALEPANARFVASRGQVRAMAMHAIEAVGAGRPRCQLCGLPMEN
                     EDEHICPATNGHRSFQE"
     gene            2139..2837
                     /locus_tag="VM841_13530"
     CDS             2139..2837
                     /locus_tag="VM841_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HVL91249.1"
                     /translation="MRAPLGDVMGLLERGDIELLGLLPRASNYTFAVKVAHQGVETLA
                     VYKPRDGETPLWDFAEGTLYRREIAAYEVSRALGWDLVPPTIARDGPYGVGSLQLFVD
                     AEPGEHYLSLLPAHADAFRRVAAFDVIVNNADRKSGHCMLEREDGAIRVVDHGVCFHE
                     EVKLRTVIWDFAGEPLPLDVVAALRGFDPASLDSLLSPSEIDAMAVRARDLIERGIFP
                     DPPEDRRAYPWPPI"
     gene            complement(2840..4258)
                     /locus_tag="VM841_13535"
     CDS             complement(2840..4258)
                     /locus_tag="VM841_13535"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVL91250.1"
                     /translation="MVSEYRTEKDSMGEVRVPAHAYWGASTQRAVDNFPVSGQRFPRR
                     FIEALGQIKWAAAAANAELGLLEAKTAEAIKAACDEVIAGTLDEHFVLDIFQTGSGTS
                     TNMNANEVIANRASEIAGGDRGSKLVHPNDHVNNGQSSNDVIPTAIHVSALAGITFDL
                     IPALERLRGALDAKAGAWTGDIKAGRTHLMDATPITFGQEFSGYAMQIRRGIERLQDA
                     SKRLAELALGGTAVGTGINADERFAARAVAILADRTGLPLREAENHFEAQGAKDAAVE
                     TSGMLRTIAASLMKIANDIRWMGSGPRAGLGELKLPELQPGSSIMPGKVNPVIPEVVT
                     QVAAQVIGNDAAIVAGGMQGHFELNVFMPMIARNMLESIQLLSAASGLFVDKCIDGLE
                     PDRERALHLAEQTFPIVTALVPAIGYDLSAEISKEAFKTGKSIREVALERGALPEKEL
                     DEALDLMKMTRGGIVAPGLGGG"
     gene            4405..5157
                     /locus_tag="VM841_13540"
     CDS             4405..5157
                     /locus_tag="VM841_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91251.1"
                     /translation="MRSRVRFILPFLLFAAMGLTVAPSHASAPTFTMADVDPSEEGYD
                     PAYVPKSVMLAPGALVRWRNDDWGSRSIHTATAYYGAAFGSSAISPGGFFSTTYDGGT
                     VLFRCMYHSELDTTISPPTCSGMCGAIHDSRQDLAPPSVSITTRDGFIFTGGVRIDGV
                     ASDDRAIKTVAVTFRPVVVAPPLLPAKSVVAICAGCDGPAVKWKVFQDAEIKGQAPFL
                     NLPPGQYRVEALATDPHGNSASAQPITIYVLR"
     gene            5225..>7006
                     /locus_tag="VM841_13545"
     CDS             5225..>7006
                     /locus_tag="VM841_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL91252.1"
                     /translation="MSDFAWTPTAAYADNARVTRLMRTHGIDDYRELVRRSQDDPGWF
                     WDAAVKDLGIEFFTPYTRVYDDARGIAWTTWFDGATVNLTHNCVDKQDPSARALVWEG
                     EDGATRTLTFGELRREVDRVANGLKSLGIGEGDRVGIQMPMVPENAVALYACAKIGAV
                     VLPVFSGFAAGAVAARLQDAGAIALITADGFYRRGSLVPLKETADEAAAASPTVKHVI
                     VFRRIGRDIGWTDGRDVEWDSLGPAEPLEAARLDPEHPLMIAYTSGTTGKPKGAVHVH
                     GGFLVKIAEEAAYQTDLGPGDLLFWFTDMGWIMGPWELIGANANGAAVLFFEGAPDYP
                     TPDRIWEIVARHKVTALGMSPTGVRALIPHGEDIVRSHDLSCLRVLASTGEPWNPEPW
                     RWYFDVVGGGRCPVINLSGGTEVGACFLSPSVLTPLRPCTLGGPSLGMAIDVYDPDGK
                     PMGPGEVGELVCTKPWPGMTRGIWNDPERYVQAYWSRWPDVWVHGDWASTDADGYWYL
                     HGRSDDTLKIAGKRLGPAEVESILVEHPGVAESAAVGVPDEVKGEKVWCFVVLKPGVE
                     PDDQLAAALRGMVADALGKAFRPDAIRF"
BASE COUNT         1048 a         2395 c         2439 g         1124 t
ORIGIN      
        1 gtcgaggacc tgacggccga ggacctcttg gcggcgatcg agcaccaggc catgcagggc
       61 gtcgactaca tgacggtgca cgccggcgtg cgtctgcgcc acctgccgct cgtgcagcac
      121 cggatcaccg ggatcgtcag ccgcgggggt ggcctgctcg cgcgctggat ggtgcaccac
      181 gggcgcgaga acccgctcta cgagcgcttc gacgacgtgc tcgagatcgc ccgccgccac
      241 gacgtgtcgc tctcgctcgg cgatgggctg cgcccgggct gcctggccga cgcctcggac
      301 cgggcccagt tcgccgagct cgacaccctc ggcgagctca cccggcgcgc ctgggacgcc
      361 gaggtgcagg tgatggtcga gggccccggc cacgtgccga tggaccagct cgaggccaac
      421 gtccggcgcc agcaggaggt ctgcggcgag gcgcccttct acacgctcgg cccgctggtg
      481 accgacgtcg ccccgggcta cgaccacatc acctcggcga tcggcgcggc gatgatcggc
      541 tcgtacggca cggcgttgct ctgttacgtc acgcccaagg agcacctcgg ccttcccgac
      601 cgcgacgacg tccgcaccgg cgtcatctcc tacaagatcg ccgcgcacgc ggcggatctg
      661 gcgaagggac atccgcgcgc gcaggagtgg gacgacgcga tctccaaggc gcgcttcgag
      721 ttccgctggg aagaccagtt cgagcttgcc cttgatcccg agaccgcgcg gagcttccac
      781 gacgagacgc tgccggccga gcccgcgaag aaggcgcact tctgctcgat gcgcatcacg
      841 caggatgtgc gcgcctacgc gaaggagcac gggatcggcg aggatcgtgt gctcgaggtc
      901 ggcttgaaag agaaggccga ggagttccgc aagaccgggg cggtgtaccg ctagcggcgg
      961 cggcgtgccc cgctagactg cgccccatgc tcttactgtt gatccgtcac gcgctcaccg
     1021 cgcgcaccgg cgtgaagctg tccggctgga caccggggat ccatctctcg aaggtggggc
     1081 gtgcgcaggc cgagagcttg gccgagcgac tcgacgcggt tccgctcacc gcgatctatg
     1141 catcgccgct ggagcgcacg gcggaaacgg cggcaccggt cgcgcgcgcg aagcgactga
     1201 aggtccggac ccgcgaggac gtcggcgagg tgcggtacgg cgacatcgag ggacgctcct
     1261 tgcgggcgct gaacaagacc aagatctgga cccagctgcg cgcctggccc tcggacgtcc
     1321 gtttccccgg cggcgagtcg ttgcgcgaga cgcaggcgcg cgcggtgacg gcgatcgagg
     1381 acatccggcg caaacaccct gacggcgtcg tggccgtctt cagccacgga gactggatac
     1441 ggctggcgat ggctcaccac atggggatcc acatcgatct ctaccggcgg ttgagcgtcg
     1501 atcccgtctc ggtcaacgtg ctccagttct acgatatggg ggtgcaggtg cgacggctga
     1561 acgagattgg gacgctcggc gatctggcgc cgggagcgaa acgatgagcg aatccttcga
     1621 cctcgacccg gtcgagcgga tcacggcggg cgcgatcggc gacccgggac agcgcacgtt
     1681 cttcgtgcaa gctgcgtcca gtttcgagca ggtgacgctg ctggcggaga aggagcaggt
     1741 gcgcatcctc gcccaggcga tcctgcagct actccagcag ctgcccggcg acgacgagga
     1801 ggacgtgtcg ccgtccttgc ttgccctcgc cgatccgctc gcgccggcgt ggcgcgcggg
     1861 agagatggcg atcgagtacg acgaggacgc cgaccgcgtc gagatcgtga tccgtgaggc
     1921 ggtccccgac gaggacgacg aagccgccgt gcccgccctc gagccggcga acgcgcgctt
     1981 cgtcgcgtca cgtggccagg tcagggcgat ggcgatgcat gcgatcgaag cggtgggtgc
     2041 ggggcgtccg cgctgccagc tgtgcggtct gccgatggaa aacgaggacg agcacatctg
     2101 tccggcgacc aacggtcacc gctccttcca ggagtagcgt gcgcgcgccg ctcggggacg
     2161 tgatgggcct cctcgagcgc ggcgacatcg aactgctcgg cttgctcccg cgggcgtcca
     2221 actacacctt cgcggtgaag gtggcccacc agggcgtcga gaccttggcg gtctacaagc
     2281 cgcgcgacgg agagacgccg ttgtgggact tcgccgaagg aacgttgtac cggcgtgaga
     2341 tcgccgccta cgaggtgagc cgcgccctcg ggtgggacct ggtcccgccg acgatcgcgc
     2401 gcgacgggcc ttacggcgtg ggttcgctcc aactgttcgt cgatgccgag ccgggggagc
     2461 attacctcag cctcctgccg gcgcacgccg acgccttccg ccgagtggcg gcgttcgacg
     2521 tcatcgtgaa caacgccgat cggaagtccg gccactgcat gctcgagcgc gaggacggcg
     2581 cgatccgcgt cgtggaccac ggggtgtgct tccacgagga ggtgaagctt cgaacggtga
     2641 tctgggattt cgccggcgag ccgctccccc tggacgtggt cgccgcgctc cgtgggttcg
     2701 atcctgcctc gctcgactcg ctgctgagcc cttcggagat cgatgcgatg gccgtgcgcg
     2761 cgcgggatct catcgaacgg ggcatctttc ccgatccccc ggaggaccgg cgcgcgtatc
     2821 cctggccgcc gatctagctt cagccgccgc cgagccccgg ggccacgatg cctccgcggg
     2881 tcatcttcat caggtcgagc gcctcgtcga gctccttctc gggcagcgcg ccgcgctcga
     2941 gcgcgacctc gcggatcgac ttgcccgtct tgaaggcctc cttggagatc tcggccgaca
     3001 ggtcgtagcc gatcgccggc accagcgccg tcacgatcgg gaaggtctgc tcggccaggt
     3061 ggagcgcccg ctcgcgatcg ggctcgagcc cgtcgataca cttgtccacg aacagccccg
     3121 acgcggccga caacagctgg atcgactcca gcatgttgcg cgcgatcatc ggcatgaaca
     3181 cgtttagctc gaaatggcct tgcatgccgc ccgcgacgat cgcggcgtcg tttccgatca
     3241 cctgcgcggc aacctgagtg accacctcgg ggatcacggg attgaccttg cccggcatga
     3301 tcgatgatcc gggctgcagc tcgggcagct tcagctcgcc caggcccgcg cgcgggcccg
     3361 agcccatcca gcggatgtcg ttggcgatct tcatcagcga ggccgcgatc gtgcgcagca
     3421 ttccgctggt ctcgaccgcg gcgtccttgg cgccctgggc ttcgaagtgg ttctcggcct
     3481 cgcgcagcgg cagcccggtc cgatcggcca gaatcgcgac cgcgcgcgcc gcgaagcgtt
     3541 cgtcggcgtt gatgccggtg ccgaccgcag tgccgcccag cgcgagctcg gccaggcgct
     3601 tcgacgcgtc ctgcagccgc tcgatcccgc ggcggatctg catcgcgtag ccgctgaact
     3661 cttggccgaa ggtgatcggg gtcgcgtcca tcagatgcgt gcggccggcc ttgatgtctc
     3721 cggtccaggc gcccgctttc gcgtccagcg cgccgcggag ccgctccagc gccgggatca
     3781 gatcgaaggt gatcccggcc agcgccgaca cgtggatggc cgttgggatc acgtcgttgg
     3841 atgactggcc gttgttgacg tggtcgttgg ggtggaccag cttcgagccg cggtccccgc
     3901 cggcgatttc gctcgcgcgg ttggcgatga cctcgttagc gttcatgttc gtcgaggtgc
     3961 ccgagccggt ctggaagatg tcgagcacga aatgctcgtc gagcgttccg gcgatcacct
     4021 cgtcgcaggc cgccttgatc gcctcagccg tcttggcctc caggagcccg agctcggcgt
     4081 tggcggccgc ggccgcccac ttgatctgcc cgagcgcctc gatgaagcgg cgcgggaaac
     4141 gctggccgct gaccgggaag ttgtccacgg cacgctgcgt ggaggctccc cagtaggcgt
     4201 gcgcggggac ccgcacctcg cccatcgaat ccttctcggt ccggtactcg ctcacgatcg
     4261 tccccttctt gacggcttga ttccctgagt ctagtgccga atcgggacaa cgcggagacg
     4321 aaaccgggaa ggcggaagaa ccgaaaccga acaggcgagg aaccgggccg gtgcccggag
     4381 aactgatccc ctggaggagg gccgatgcga tccagggttc gtttcatcct cccattcctg
     4441 ctttttgcgg cgatgggact gacggtcgcg ccgtcgcatg ccagcgcgcc caccttcacc
     4501 atggcggacg tggatcccag cgaggaaggc tacgatcccg cctacgttcc caagtccgtg
     4561 atgctcgcgc cgggagcgct cgttcgttgg cgcaacgacg actggggctc gcgttcgatc
     4621 cacacggcga ccgcctacta cggcgcggcg ttcggctcct ccgcgatcag cccgggcgga
     4681 ttcttctcga ccacctacga cggcgggacg gtgctgttcc gctgcatgta ccactccgag
     4741 ctggacacca cgatctcgcc gcccacgtgc tcgggcatgt gcggggccat ccacgactcc
     4801 cgccaggacc ttgcgccgcc gagcgtatcg atcaccacgc gggacgggtt catcttcacg
     4861 ggcggtgtcc gcatcgacgg cgtcgcctcc gacgatcgcg cgatcaagac ggtcgcggtg
     4921 accttccggc ccgtggttgt cgcgccgccg ctgctgcccg ccaaaagcgt cgtcgcgatc
     4981 tgcgccggct gcgacggacc ggccgtgaag tggaaggtct tccaggacgc cgagatcaaa
     5041 ggtcaggccc cgttcttgaa cctgccgccc gggcagtatc gggtggaggc gctggccacc
     5101 gatccccacg gcaactccgc ctcggcgcag ccgatcacga tctacgtgct gcgctgatcg
     5161 ctgccgccgc ccgatcggtg gcccctttgc ccggcgctcg ccgaggccga tacgctcgcc
     5221 gtccatgagc gacttcgcct ggacgcccac tgctgcttat gccgacaacg cgcgcgtcac
     5281 gcggctgatg cgcacccacg ggatcgacga ctaccgcgag ctggtccgtc gctcgcagga
     5341 tgatccgggc tggttctggg acgcggcggt gaaggatctg ggcatcgagt tcttcacgcc
     5401 gtacacgcgt gtctacgacg acgcgcgcgg catcgcatgg acgacctggt tcgacggcgc
     5461 gaccgtgaac ctgacccaca attgcgtgga caagcaggac ccgtcggcgc gggcgctggt
     5521 gtgggaaggc gaggacggcg cgacacgcac gctgacgttc ggcgagctgc gacgcgaggt
     5581 cgatcgcgtc gcgaacgggc tgaagtcgct gggcatcggc gaaggggatc gggtcgggat
     5641 ccagatgccg atggtgcccg agaacgccgt cgcgctgtac gcgtgcgcca agatcggcgc
     5701 ggtcgtgctg ccggtcttca gcggcttcgc cgccggtgcc gtcgcggcgc ggctgcagga
     5761 cgccggcgcg atcgcgctca tcaccgccga cggcttctat cggcgcggca gcctcgtgcc
     5821 gctgaaagag actgccgacg aggccgcggc cgcctcgccg accgtcaagc acgtgatcgt
     5881 gtttcgccgc atcggccgcg acatcggctg gaccgacggc cgggacgtcg aatgggactc
     5941 gctggggccg gccgagccgc ttgaggccgc gcgtctggat cccgagcatc cgctgatgat
     6001 cgcctacacg tccggcacga ccggcaagcc gaagggtgcg gtgcacgtgc acggcggctt
     6061 tctggtcaag atcgccgaag aggccgcgta ccagaccgat ctgggtcccg gcgatctgct
     6121 gttttggttc accgacatgg gctggatcat gggcccgtgg gagctgatcg gcgccaacgc
     6181 caacggcgcg gcggtcctgt tcttcgaagg cgcgcccgac tatccgacgc ccgatcgcat
     6241 ctgggagatc gtcgcccggc acaaggtcac cgcgctgggc atgtcgccga ccggcgtgcg
     6301 cgcgttgatc ccccacggcg aagacatcgt ccgctcgcac gacctgtcgt gccttcgcgt
     6361 gctggcatcg accggcgagc cgtggaatcc cgagccgtgg cgctggtact tcgacgtcgt
     6421 cggcggcgga cgctgcccgg tcatcaacct cagcggcggc accgaggtcg gcgcgtgctt
     6481 cttgtcgccg tcggtgctca cgccgcttcg cccgtgcacg ctgggcgggc cgtcgctggg
     6541 catggccatc gacgtctacg atcccgacgg caagccgatg gggcccggcg aggttggcga
     6601 gctggtgtgc accaagccgt ggcccggcat gacgcgcggc atctggaacg atcccgagcg
     6661 ttacgtgcag gcgtactggt cgcgctggcc cgacgtgtgg gtgcacggcg actgggcctc
     6721 gaccgatgcc gacggctact ggtatctgca cggccgcagc gacgacacgc tcaagatcgc
     6781 gggcaagcgg ctggggccgg ccgaggtcga gtcgatcctg gtcgagcatc ccggcgtcgc
     6841 cgaatccgcg gccgtcggcg tgcccgacga ggtcaagggc gagaaagtct ggtgtttcgt
     6901 cgtgctcaag cccggcgttg aacccgatga tcaattggcc gcggcgctgc gcggcatggt
     6961 ggccgacgcg ctcggcaagg cgttccggcc cgacgcgatc cggttc
//
LOCUS       DATLFO010000336         4416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_117541, whole genome shotgun sequence.
ACCESSION   DATLFO010000336 DATLFO010000000
VERSION     DATLFO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117541"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VM841_13550"
     CDS             complement(<1..195)
                     /locus_tag="VM841_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HVL91253.1"
                     /translation="MSDPEREDSVGVGPHPRPWPDDPRLDPALLAEGDARNVVDRYRY
                     WRMDAIAEDLDARRHPFHVAI"
     gene            complement(297..2105)
                     /locus_tag="VM841_13555"
     CDS             complement(297..2105)
                     /locus_tag="VM841_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVL91254.1"
                     /translation="MRSSRRFSSLVLSLLIGIAAAPARSASGRAITPADEPIANSYIV
                     TLAGSPAGDRVPSVARTLTTRFGGTVARTYRHALKGFSLRTSAAAARAMAASPLVAAI
                     EQDGVVRIGATQTGATWGLDRIDQRSLPLNGTYTSNATGSDVRVYVIDTGIRAAHTDF
                     GGRASVGADMVGDGQNGNDCNGHGTHVAGTAAGTTFGVAKQAKIIGVRVLGCTGNGST
                     SGVIAGVDWVTANRVLPAVANMSLGGGASNALDAAVRSSIASGVSYALAAGNGNLLGL
                     PENACNVSPARVAEGLTVGATDSADRKASFSNYGSCLDVFAPGVNITSAWSTSNTATN
                     TISGTSMAAPHVAGVAALYLQSNRSASPAQVASALTASATGDAVASAGSGSPNRLLYA
                     AVTEGAPPANQAPTAGFSFSCSGLSCSFSDASSDPDGTIVARSWQFGNGATSTATNPA
                     YTYPAGGTYTITLTVTDDDGATSSVSKSVTVAAGGDPDPGTPTLTSGVARSDTSGAAG
                     TWKFFKIEVPAGRGQLKVALTSSQSCGLLGCNPDLDLYVRHGAKPTASTFTCRPYTGS
                     SSESCTLNGPAAGWWYIGVYVYSGSASTSYTVKATY"
     gene            2350..3483
                     /locus_tag="VM841_13560"
     CDS             2350..3483
                     /locus_tag="VM841_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HVL91255.1"
                     /translation="MRNRLIVIIVVAALAGGAAALIAMRDGTRDAAVGEKVVLAFGDS
                     VAAGQGLGPALGYPNNDQAYPARLGALLGWRSMNFAVSSACAASADDPGAHPDTPGTC
                     QASVLRDQIPHAAETFKGSPRVVVLTVGANDIRYADCGLQVIVPELFDDKCDDATLDR
                     RLVALEANLGRVLDRLRRGYPGSVIVLTSYYNPMPAPVRDGEEACPIYEPLAALKSPV
                     TLVSERALRAATADLQAQVHVRFREGVTRLNESIARAAIAGDAIVAPADFTGHDLCRS
                     ETGGGQAWVYGPDYDIRFRLKGAASLLGARKTWRGTLPSRCPSPSPAEPPEHSTGEVE
                     RRVGVGTVIYSARVNVNCMPHPTAEGQQALAAAVLAALPEPVR"
     gene            3521..4255
                     /locus_tag="VM841_13565"
     CDS             3521..4255
                     /locus_tag="VM841_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HVL91256.1"
                     /translation="MSRRRAVLALLGFVVVATCASLGAWQLNRLADRRALNDRLIARE
                     ALPVAEAATLEMRGGRADPAAYRRVRATGVFDVSQEVVLRARPLNGRPGNHVLTPLRL
                     GDGSAIVVNRGWVPLRMDEPGAAEAAPPASQVTVTGILVPSEPAPVVFGPRDPATGRL
                     ATIGRIDLERLRAQIPYSIAPLAIAMESQQPSHAALPEPAGRPPPGEGPHLAYAVQWF
                     IFAVIALVTTALLMRREREAAASPSA"
     gene            complement(4197..>4416)
                     /locus_tag="VM841_13570"
     CDS             complement(4197..>4416)
                     /locus_tag="VM841_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91257.1"
                     /translation="PGWAAFFPIRIVLLAIRTAERGAGRSFELARNNRFIGVLAAMLG
                     AAIVASVVWLTLTATRRLRAPAASAEPS"
BASE COUNT          643 a         1491 c         1607 g          675 t
ORIGIN      
        1 gatcgcgaca tggaaggggt ggcgccgcgc gtcgagatcc tccgcgatcg cgtccatccg
       61 ccagtagcgg tagcggtcca cgacgttgcg cgcgtcgccc tcggcgagca gcgccgggtc
      121 caggcgcgga tcgtcgggcc acggccgggg atgcgggccg acgccgacgg aatcctcgcg
      181 ttccggatcg ctcatcgtgc gtatgatcgc acgcctcgtg gtgacagggg tcccgggacg
      241 aagagcacgg aaacgaagag ggagccgcgc gcggctccct cttcgaaacg gctctcctag
      301 tacgtcgcct tgacggtgta gctcgtggac gccgagcccg agtacacata gaccccgatg
      361 taccaccagc cggcggcggg tccgttcagc gtgcaggact cgctcgacga tcccgtgtag
      421 ggacggcatg tgaacgtgct cgccgtgggc ttggctccgt gacgcacgta caggtccaga
      481 tccgggttgc agcccagaag gccgcacgac tgcgacgagg tcagggcaac cttgagctgg
      541 cctcggccgg ccgggacctc gatcttgaag aatttccacg tcccggccgc gcccgaggtg
      601 tcgctgcgcg cgaccccgct cgtgagcgtc ggcgtgccgg gatcggggtc gccgcccgcg
      661 gcgacggtga cgctcttgga caccgaggat gtcgcgccgt cgtcatcggt cacggtcaag
      721 gtgatggtgt aggttccgcc cgccggatag gtgtacgcgg ggttcgtcgc ggtcgaggtc
      781 gctccgtttc cgaactgcca cgagcgcgca acgatcgttc cgtcggggtc gctgctcgcg
      841 tcgctgaacg agcacgacag gcccgagcac gagaaggaga accctgccgt gggggcctga
      901 ttcgctggcg gagcgccctc agtgaccgcg gcgtagagca gacggttcgg cgagccgctg
      961 ccggccgagg cgacggcgtc gccggttgcg gacgccgtca gcgcagacgc cacctgagcg
     1021 ggcgacgccg agcggttgga ctgcaggtac agcgcggcga cgccggcgac gtgcggagcg
     1081 gccatggacg tgccgctaat cgtgttggtc gccgtgttcg aggtggacca cgcgctggtg
     1141 atgttcacgc caggcgcgaa cacgtccagg cacgagccgt agttcgagaa cgacgccttg
     1201 cggtcggccg agtcggttgc gccgacggtt aagccttcgg cgacgcgggc gggtgagacg
     1261 ttgcaggcgt tctcgggcag cccgaggagg ttgccgttgc ccgcggcgag cgcgtaggag
     1321 acgccggagg cgatcgagct gcgaaccgcg gcgtcgagcg cgttgctcgc cccgcccccg
     1381 aggctcatgt tcgcgacggc gggaaggacg cggttggcgg tgacccagtc cacgccggcg
     1441 atcacgcccg acgtggatcc gttgccggtg catccgagca cgcgaacgcc gatgatcttc
     1501 gcctgcttcg ccacgccgaa ggtcgtgccc gcggcggtcc cggcgacgtg cgtgccgtgg
     1561 ccgttgcagt cgtttccgtt ctgaccgtcg ccgaccatgt ccgctcctac cgaggcgcgg
     1621 ccgccgaaat cggtgtgcgc tgcgcgaata ccggtgtcga tgacgtagac gcggacatcg
     1681 ctgcccgtcg cgttggaggt gtaggtgccg ttgagcggca gcgaacgctg atcgatgcgg
     1741 tccaagcccc aggtcgcccc ggtttgcgtc gcgccgatgc gaacgacgcc gtcctgctcg
     1801 atggccgcca ccagcgggct cgcggccatc gcgcgtgcgg ccgccgcaga ggtgcgaagc
     1861 gagaatccct tgagcgcgtg acggtacgtg cgtgcgacgg tgccgccgaa tcgggtggtc
     1921 agcgtgcgag cgacggacgg gacacggtcg cctgccgggc taccggcgag cgtgacgatg
     1981 taggagttcg cgataggctc gtcggcgggc gtgatcgcgc gtccggacgc gctacgcgcc
     2041 ggagcggcgg caatgccgat caacagcgaa agaacgagcg acgaaaagcg gcgggacgat
     2101 ctcatgtgcg ggcaacctcc gatgcggcgg tggaaaagtg atgctacgcc gcgaagccga
     2161 gcgagcgagg gcaaaaatcc acgttttctt caatacccca acgcttctta gacgactcgc
     2221 ggggcaaatg gtgacgcttc gcccttgctg gccgccggat tgcgtcgatg tgaccggcgt
     2281 gcccgcggca tccaaacgcg tcacaatgca tttcgctcga gcgcaaaccg tgcaatcggt
     2341 gatgatccgt tgcgaaaccg gctgatcgtg atcatcgtgg tcgctgccct tgcgggcggc
     2401 gccgctgcgc tcatcgcgat gcgtgacggg acgcgggacg cggccgtggg cgagaaggtg
     2461 gtgctcgcct tcggtgactc ggtggcggcg ggccaagggc tcggtcccgc gctcggctat
     2521 cccaacaacg accaggcgta tccggcgcgg ctgggtgcgc tgctcggatg gcgatccatg
     2581 aacttcgcgg tctcgtcggc gtgcgcggcg tcggcggatg atccgggtgc gcatccggac
     2641 acacccggga cgtgtcaggc atccgtccta cgtgaccaga ttccgcacgc cgccgagacc
     2701 ttcaaaggat cgccgcgcgt ggtcgtgctg accgtgggcg cgaacgacat ccgctacgct
     2761 gattgcggcc ttcaggtgat cgtgcccgag ctcttcgacg acaagtgtga cgacgcgacg
     2821 ctcgaccggc gtctcgtcgc gctcgaagcg aacctcggtc gcgtgctgga ccgtctgcgc
     2881 cggggctatc ccggctccgt gatcgtgctg acgtcctact acaacccgat gcccgcgccc
     2941 gtgcgcgacg gcgaggaggc gtgtccgatc tacgagccgc tcgcggcgct caagagcccg
     3001 gtgacactgg tgtcggaacg ggcgttgcgc gccgcgaccg ccgacctgca agcccaggtg
     3061 cacgtccgct tccgcgaggg cgtgacgcgt ctcaacgaga gcatcgctcg cgccgccatc
     3121 gcgggtgacg caatcgtggc tcccgccgac ttcaccgggc acgacctgtg ccgctccgag
     3181 accggcggcg ggcaggcctg ggtctacggg cccgactacg acatccgctt tcgattgaag
     3241 ggcgcggcat cgcttttggg cgcccgcaag acctggcgcg gcacgttgcc gtcccgctgt
     3301 ccgtcgccgt ctccggcaga gccgccggag cactcgaccg gcgaggtcga gcggcgcgtc
     3361 ggtgtgggaa cggtgatcta ctcggcgcgc gtgaacgtca actgcatgcc gcacccgacg
     3421 gccgaagggc agcaggcgct cgccgccgcc gtcctggccg cgcttcccga gcccgtgcgg
     3481 tgacgcgcac gcggacgggg gcggctatcc tgccgcaccg atgagcagac gacgcgccgt
     3541 cctcgcgctt ctgggattcg tcgtggtcgc gacctgcgct tcgctcggcg cctggcagct
     3601 gaaccggctc gcagaccggc gcgcgctcaa cgaccggctg atcgcgcgcg aagcgttgcc
     3661 cgtcgcggag gcggcgacgc tcgagatgcg cgggggccgc gccgatcccg ccgcgtatcg
     3721 ccgtgtccgc gccaccggcg tgttcgatgt gtcgcaggag gtcgtcctgc gggcgcggcc
     3781 gttgaatgga cgcccgggca atcacgtgct gacgcccctg cgtctcggcg acggcagcgc
     3841 gatcgtggtg aaccgcggct gggtgccgct gcgcatggac gagcccggag cggccgaggc
     3901 ggcgccgccg gcgagccaag tgacggtaac cggcatcctc gtgccgtccg agcccgcccc
     3961 ggtcgtgttc ggcccgcgcg acccggcaac gggacggctg gccaccatcg gccggatcga
     4021 cctggagcgg ctgcgcgcgc agattcccta ttcgatcgcg ccgctcgcga tcgcgatgga
     4081 gtcccagcag ccttcgcacg cggccttgcc cgagccggcg gggcgtccgc cgccgggcga
     4141 gggcccgcac ctggcctatg cggttcagtg gttcatcttc gccgtgatcg cgctcgtcac
     4201 gacggctctg ctgatgcggc gggagcgcga agccgccgcg tcgccgtcag cgtgagccag
     4261 accacgctcg cgacgatcgc cgcgccgagc atcgccgcga gcacgccgat gaatcggttg
     4321 ttgcgcgcga gctcgaagga ccggccggcg ccgcgctcgg ccgtgcggat ggccagcagc
     4381 acgatgcgta tcggaaagaa cgcggcccat cccggt
//
LOCUS       DATLFO010000337         3783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_78355, whole genome shotgun sequence.
ACCESSION   DATLFO010000337 DATLFO010000000
VERSION     DATLFO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78355"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VM841_13575"
     CDS             <1..749
                     /locus_tag="VM841_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91258.1"
                     /translation="EDTEAAEVLESVFERRGMTIVKRARAASAVASGDGVLVTLEDGR
                     VLEGSHALMTVGMVPNSRELGLEDAGVRVNDWGAIEIDGVSRTSAPGVYAAGDVTGHM
                     MLASVAAMQGRIAMWHALGQAVTPMRYEAVASTVFTDPEIATVGLSQTKASERGVHVD
                     ALTLPLSTNARAKMVGLEDGFVKIVCVPGAGTVLGGTIVSPHASDLILPLSVAVHSRL
                     TVGQLAQAFSIYPSLGGSVQEAARQLGRSS"
     gene            complement(778..1329)
                     /locus_tag="VM841_13580"
     CDS             complement(778..1329)
                     /locus_tag="VM841_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91259.1"
                     /translation="MSVLAQRLLAVHDALDAAALPHAFGGAIALAYCIEEPRGTRDID
                     VNIFVPSSRIDEALDAMPAGIVVTATQRATAHRDGQVRLFWDDTPVDVFFDTDEFHKH
                     AAAGIGPVPFFGRTIPVLGCVALVVFKAMFDRTKDWADIEAVAAANTPPTRQAAEHLA
                     RILGPDDPRIERLHTLISEIEAL"
     gene            complement(1326..1634)
                     /locus_tag="VM841_13585"
     CDS             complement(1326..1634)
                     /locus_tag="VM841_13585"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVL91260.1"
                     /translation="MGRAAGLIIEARRRAGISQAELARRAGLPRSVLNAYEHANREPG
                     ADTLIRILAAAGFDVHLQPRIDVERNARILEQVLELAEGLPYRPRKTLAYPRFKRQAG
                     "
     gene            complement(1684..2247)
                     /locus_tag="VM841_13590"
     CDS             complement(1684..2247)
                     /locus_tag="VM841_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVL91261.1"
                     /translation="MTAVWIILGVVVLLGLAIAISYNRFVSQKNLIRNAWANIDTELR
                     RRYDLIPNLVETVKGYASHERQTFEAVTAARNAAVAATGSPAQQAQAEGPLVGALRQL
                     FAVAENYPQLRANENFLQLQGELTNTEDRLQTARRFYNNNVQDYNRRVQSVPSNIIAN
                     MFNFKEEEFFEVEEAIRTAGAPKVDFS"
     gene            complement(2287..3021)
                     /locus_tag="VM841_13595"
     CDS             complement(2287..3021)
                     /locus_tag="VM841_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91262.1"
                     /translation="MQALFFLLIALIIGAGFYVGWHLKQKRRDELAALASRLGMTFAA
                     SDPFGIDDQMPHQLFSIGDGRGCENVMWGVWNGMPATAFDYWYYTESTDSEGHTSRTY
                     HRFSCAMTYVPADCHHLVITKENLLTRMADGLGFRDIQFETGEFNKAWQIKCPDRKFA
                     TDFIDQRMMQWLMYAGTQWSFEVVGQIGLVYCRKLGTGEIPALLGCLKAFRDKIPRVV
                     SDLYPLPPDAPQMPGVRPVGDTAAQG"
     gene            complement(3140..3562)
                     /locus_tag="VM841_13600"
     CDS             complement(3140..3562)
                     /locus_tag="VM841_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL91263.1"
                     /translation="MWPIVLLSLAMNLLAPATRDVVTISYEGHLHALINQTRDAQGLA
                     SLAVDASLTRVARAHSDRIAAAGVVSHTPSLHEVLGGWTVLGENVAMASTVGDVFFYF
                     MDSAKHRALIQDAGFTHMGAGVTDASGTLFVTVWFGRR"
     gene            complement(3620..>3783)
                     /locus_tag="VM841_13605"
     CDS             complement(3620..>3783)
                     /locus_tag="VM841_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HVL91264.1"
                     /translation="GDDLGFDEAEVVTYDALVEAGSWNAAKAKGLVRQEGKTYVMQEG
                     DVVEFRFAV"
BASE COUNT          612 a         1149 c         1365 g          657 t
ORIGIN      
        1 ccgaagacac cgaggcggcc gaggtgctcg agtcggtgtt cgagcgccgc ggcatgacca
       61 tcgtcaagcg cgcccgcgcc gcctccgccg tcgcatccgg cgacggggtc ttggtcacgc
      121 tggaggacgg ccgcgtgctg gaaggctcgc acgcgctgat gaccgtcggc atggtgccga
      181 actcgcgcga gctgggtctg gaagacgcgg gcgtgcgcgt caacgattgg ggcgcgatcg
      241 agatcgacgg cgtgtcgcgc accagcgcgc ccggcgtcta cgccgccggc gacgtcaccg
      301 gccacatgat gctcgccagc gtcgccgcca tgcagggccg catcgccatg tggcatgcgc
      361 tcggccaggc ggtcacgccg atgcgctacg aggcggtcgc ctcgaccgtg ttcaccgacc
      421 ccgagatcgc gacggtcggc ttgtcgcaga ccaaagccag tgagcgcggc gtgcacgtcg
      481 atgcgctcac cctgccgctg agcacgaacg cgcgggccaa gatggtcggc ctggaagacg
      541 gcttcgtgaa gatcgtctgc gtgccgggcg ccggcaccgt gctcggcgga acgatcgtgt
      601 caccgcacgc cagcgacctg atcctgccgc tatcggttgc cgtgcactcc cgcctgaccg
      661 tgggccagct cgcccaagcg ttctcgatct acccgtcgct cggcggcagc gtccaagaag
      721 ccgcccgcca gctcggccgc tcgagctgat cggcgtccac tcgatcgggt tgcgacctta
      781 gagagcttcg atctcgctga tgagcgtgtg cagccgctcg attcggggat cgtcggggcc
      841 gagaatgcgg gcgagatgct cggcggcttg gcgggtgggg ggggtgttcg cggcggcgac
      901 ggcttcgatg tcggcccagt cttttgtccg gtcgaacatg gctttgaaga cgaccagggc
      961 gacgcatccg aggaccggga tggtgcggcc gaagaagggc acggggccga tgccggcggc
     1021 ggcgtgcttg tggaactcgt cggtgtcgaa gaagacgtcg acgggcgtgt cgtcccagaa
     1081 caggcggact tggccgtcgc ggtgggcggt ggcgcgttgc gtggcggtca cgacgatgcc
     1141 ggcgggcatc gcatcgagcg cctcgtcgat gcgcgatgag ggcacgaaga tattgacgtc
     1201 gatgtcgcgt gttccgcgcg gctcttcgat gcagtaggcg agggcgatcg cgccgccgaa
     1261 cgcgtggggg agagctgcgg cgtcgagcgc atcgtggacg gccagcagcc gttgcgcgag
     1321 gacgctcatc cggcctgccg tttgaatcgg ggataggcga gtgtcttgcg cggccggtag
     1381 ggcagacctt cagcgagttc gagaacctgc tcgaggatgc gtgcgttgcg ttccacatcg
     1441 atgcgcggct gcaggtgaac gtcgaagccc gcggcggcca ggattcggat gagcgtgtcg
     1501 gcgccgggtt cgcggttggc gtgctcgtag gcgttgagaa ccgatcgtgg caggccggcg
     1561 cgccgggcca gttcggcttg ggagatgccg gcgcggcggc gggcttcgat gatcagtccg
     1621 gcggcacggc ccatacggtc gatgttagcg aatccgctaa catcgagtca atcgggccgg
     1681 tgattagctg aagtcgacct tgggggcgcc ggcggtgcgg atcgcctctt cgacttcgaa
     1741 gaactcttct tctttgaaat tgaacatgtt ggcgatgatg ttggacggca ccgactggac
     1801 gcggcggttg tagtcctgaa cattgttgtt gtagaagcgg cgcgcggtct gcagccggtc
     1861 ttcggtgttg gtcagctcgc cttggagctg caggaagttt tcgttggcgc gcagctgcgg
     1921 gtagttttcg gcgacggcga acagctggcg cagcgccccg acgagcggcc cctccgcttg
     1981 cgcctgctgg gcggggctgc cggtggcggc gacggcggcg ttgcgggcgg cggtgacggc
     2041 ttcgaaggtc tgacgctcgt ggctggcgta gcccttgacg gtctcgacca ggttcgggat
     2101 caggtcgtag cggcggcgca gctcggtgtc gatgtttgcc caggcattgc ggatcaggtt
     2161 cttctgcgac acgaaccggt tgtaggagat cgcgatcgca aggcccagca ggaccacgac
     2221 tccgaggatg atccagactg ccgtcacgtc ggctccttcc gttggttccg cccgaggcga
     2281 accgtatcag ccttgcgcgg cggtgtcacc gacgggccgc acgcccggca tctgcggcgc
     2341 gtcgggcggc agcggataca gatcggatac gacccgcggg atcttgtcgc gaaacgcctt
     2401 gaggcagccc agcaacgcgg gaatctcgcc ggtgccgagc ttgcggcagt agaccaggcc
     2461 gatctggccg acgacctcga aagaccattg cgttcccgcg tacatcagcc attgcatcat
     2521 gcgctgatcg atgaagtcgg tggcgaactt ccggtcgggg cacttgatct gccaggcctt
     2581 gttgaactcg ccggtctcga attggatgtc gcggaagccc aggccgtcgg ccatgcgggt
     2641 cagcagattc tctttggtga tgaccagatg gtggcagtcg gccggaacgt aggtcatcgc
     2701 gcagctgaag cgatggtagg tgcgcgaggt atggccttcg gagtccgtgg actcggtgta
     2761 gtaccagtag tcgaacgcgg tggcgggcat gccgttccag acgccccaca tcacgttctc
     2821 gcagccgcgg ccgtcgccga tcgagaacag ctggtgcggc atctggtcgt cgatgccgaa
     2881 gggatcggac gcggcgaagg tcatgcccag ccgcgacgcg agcgcggcca gctcgtcgcg
     2941 acgcttctgc ttgagatgcc agccgacgta gaaccccgcg ccgatgatca gcgcgataag
     3001 aaggaagaac agggcctgca taacggctcg aagtatctca gatcgggggg atctcgtccc
     3061 ggcagatcgt tcttggtcaa tttggggccc tataggtgca cgaagttgac caagaacgat
     3121 ccgaggatcg ggtttcgggt cagcgtcggc cgaaccagac ggtgacgaac agggtgccgc
     3181 tggcgtcggt caccccggcc cccatgtgcg tgaatcccgc gtcttggatg agggcccgat
     3241 gcttggccga atccatgaag tagaagaaca cgtcaccgac cgtcgacgcc atcgccacgt
     3301 tctcacccag cacggtccag ccgccgagga cctcgtgaag gctcggcgtg tgcgacacga
     3361 cgccggctgc ggcgatgcgg tccgagtggg cgcgcgcaac gcgggtcagc gacgcatcga
     3421 cggccaggga agcgagacct tgggcatcgc gggtctggtt gatcaaagcg tgcaggtggc
     3481 cttcgtacga gatggtcacc acgtcgcgcg tcgcgggcgc gagcaggttc atcgcgaggg
     3541 acagcagcac gatcggccac acaggagtct ccttcccggg acatcatgcc acgaaacgga
     3601 ccgagcgggc cgggggaggc tagacggcga agcggaactc gaccacgtcg ccttcctgca
     3661 tgacgtaggt cttgccttct tggcggacca ggcccttggc cttcgcggcg ttccaggatc
     3721 cggcttcgac gagcgcgtcg taggtgacga cctcggcttc atcaaagccg aggtcgtcac
     3781 cta
//
LOCUS       DATLFO010000338         6845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_67554, whole genome shotgun sequence.
ACCESSION   DATLFO010000338 DATLFO010000000
VERSION     DATLFO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67554"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VM841_13610"
     CDS             <1..736
                     /locus_tag="VM841_13610"
                     /inference="COORDINATES: protein motif:HMM:NF018651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HVL91265.1"
                     /translation="LTGVDFLCAHLQALAPVVPQLSLEVQVWSQDEYARLRVAGCDGV
                     VVYQETYDRDSYAHFHTAGRKRDYDWRLEAPERAAAAGIRRLGIGALLGLHEPWREDA
                     IATAAHALHLLKVAWRCEIAVSMPRIRPSASGYSPEHPVTDTELALYVCALRLLMPDV
                     ALVGSSREEASYRDGLVRLGITHTSAGSHTEPEGYTHPDEATQQFEVSDTRSPEEVAA
                     ALRAMGYDPVWKDWDAGHRAPAEAAR"
     gene            733..939
                     /gene="thiS"
                     /locus_tag="VM841_13615"
     CDS             733..939
                     /gene="thiS"
                     /locus_tag="VM841_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HVL91266.1"
                     /translation="MTITANGKPREIPDGITIRAFLDTLGWKPGWVVVEHNGEPLDRA
                     RYDAVALRDGDRIEVVRAVAGGAR"
     gene            936..1565
                     /gene="thiE"
                     /locus_tag="VM841_13620"
     CDS             936..1565
                     /gene="thiE"
                     /locus_tag="VM841_13620"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVL91267.1"
                     /translation="MTARRERLDAARLYLVCGGQRPDLDEFLEAALTAGVDVVQLREK
                     QMEARPLLEIAARFRRACDRHGALLVINDRPDLAIASNADGVHLGQDDLPPAAGRALV
                     GNEMLIGRSTHAPDETRAALSEPVDYICVGPVHATPTKPGRSATGLDLVRLAAAECAK
                     PWFAIGGLDPQTAKEAAQAGATRIVVVRAIEQAADVEGAVRALRSAIGA"
     gene            1640..2539
                     /locus_tag="VM841_13625"
     CDS             1640..2539
                     /locus_tag="VM841_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91268.1"
                     /translation="MKRLMAAIVLVLALGACEARAEIDVNDNGSGTFGFAFFIDKQFI
                     ALMEGFGGRQGEDPFGDFKSGLDDSPVPFQIEEYSENGGRGIRAKVPFKSVDDLRTIM
                     KEVDQGDAQGSPLGGGGPGFGTFTLEQRGGGWYFESLSDAPDLGDLGDLGSGEGGGEA
                     PFDPAQIASLLKISFRVTLPGRTVDTTADEATSKGGKTTFVWNADLTSKEPMQMVATT
                     AAAGSSLPLSPILIALALIAALVTVAVMMRRKNAVASGPDQHADAWPSGTGGAVPPQP
                     GTVPPVSGTHEQDPQRPLEPPAP"
     gene            2614..3117
                     /locus_tag="VM841_13630"
     CDS             2614..3117
                     /locus_tag="VM841_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91269.1"
                     /translation="MKPASIVTAGVALSLSLIACGGSDGRVAAPAFTPTEQQTAGDDP
                     VSAKPACDYVTEAEASQVFETEMKYRSNDGGTNCIFDGSPDGSASIDYTISDGTDGYD
                     IVSDGMQPVDGIGDRAAGRDGSGLSQVVFTKGNTMVTLTVSGNDIKGGGPFDVAKKFA
                     LFLAGKL"
     gene            complement(3121..4266)
                     /locus_tag="VM841_13635"
     CDS             complement(3121..4266)
                     /locus_tag="VM841_13635"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HVL91270.1"
                     /translation="MSDYEFETRAIHTGQDPDPASGAVIPPVSFSTTFAQSAVGEHKG
                     YDYARSGNPTRAALEACLASLEGAAHGLCFASGLAATDAALRLINPGDHLIIGDDAYG
                     GTYRLVAQVFAPQGYEFTPVNMGDPAAIEAAWRPNTKLVVMETPTNPLLKIVDIAAVA
                     AITRDRGARLIVDNTFATPYLQQPLSLGADIVLHSATKYLGGHSDVVGGFLATDDDET
                     FERLKFLQNAVGAVPGPLDCYLVLRGVKTLAIRVERQCANAREIVAMLQQHPAVTTVL
                     YPGVPTHPGHEIAAKQMRDFGGMVSFLAAGGEQAALDIVARTKIFTLAESLGAVESLI
                     EHPGKMTHASVAGSPLEVDASLVRLSVGIENARDLVADLKQALDSVR"
     gene            complement(4292..5518)
                     /locus_tag="VM841_13640"
     CDS             complement(4292..5518)
                     /locus_tag="VM841_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL91271.1"
                     /translation="MADSEIDQLTAELGAIIDRVQRFASRGGERRASLAVAALRAADR
                     LTAAAATTISRGGFASGAGVPAPIMLQMDARATSWDARALTRSAAMLDSMPNSAHAFQ
                     EGLLSWSQVRGICNAARSCDAAGRRAVDRLVARDALFLRDADPDQIVRDVEDLVAELK
                     HRREERHEQQAVDRSFVTFQPRLDGRTFFYGEGDTVSVATLIEAADTAAAAPSPADTL
                     AQRRYDALIAIAEQHLGGSSGSNLPTRSRPRLLATIDVADLTRDRREASARILWAIAG
                     RAPRLSPLGTETLACDADVLPILFERGTPVAVGDATSPIPPKIRRAVAARDRGCRFPG
                     CNAPLSWADAHHIRARRDGGTADPDNLVMLCRRCHHRVHRRGWTITRDTTGSLYFARR
                     GARYHSPLPGRAPPRE"
     gene            complement(5654..6268)
                     /locus_tag="VM841_13645"
     CDS             complement(5654..6268)
                     /locus_tag="VM841_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91272.1"
                     /translation="MATSSRRVAVDRLRVPFLIVGACSAGVTLAGSLLYFMGPGISSF
                     YGRLPGWLTLARAGVGLVLPAGFRMTLIERVIFLMLAASLSLVVWFRLRRVSAPIAVI
                     VSSLWIAAAIAAPAYSFGLYNGHPDALPYVDGLGTWCVYRHCALIDYIGWPYAALGMQ
                     LAVAAIAAVVYVWPRKRRWLAGSMTVLFALSMILVGNGLERVVR"
     gene            complement(6519..>6845)
                     /locus_tag="VM841_13650"
     CDS             complement(6519..>6845)
                     /locus_tag="VM841_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91273.1"
                     /translation="RVPAAIARVVSGLWIAAAIAAPTYSFNLYDGPLENFGTNYGIAG
                     SSMIPVVWTRWPYAALGMQLAVVAIAAAVHAWRKERRWLRVGSMTVLFALSMILVGNG
                     LKRVVG"
BASE COUNT         1134 a         2295 c         2374 g         1042 t
ORIGIN      
        1 gctcaccggc gtggacttct tgtgcgcgca cctgcaggcg cttgcgccgg tcgtgccgca
       61 gctttcgctc gaggtgcagg tctggtcgca ggacgagtac gcgcggctgc gcgtcgcggg
      121 atgcgacggc gtcgtggtct accaagaaac ctacgatcgc gattcttacg cgcatttcca
      181 caccgccggg cgaaagcgcg actacgactg gcggctggag gcgcccgagc gcgcggcggc
      241 ggcgggcatt cgccggctcg gcatcggcgc gctgctcggc ctgcacgagc cgtggcgcga
      301 ggacgcgatc gccaccgccg cgcacgcgct gcatctgctc aaggtcgcgt ggcgctgcga
      361 gatcgcggtc agcatgccgc gtatccgtcc gagcgcgagc ggctactcgc ccgagcatcc
      421 ggtcaccgac accgagcttg cgctgtacgt gtgcgcgctg cgcctgctga tgcccgacgt
      481 tgcgctcgtg ggctcctcgc gcgaggaggc gtcgtaccgc gacgggctcg tgcggctcgg
      541 gatcacgcac acctcggccg ggtcgcacac cgagccggag ggctacacgc atcccgacga
      601 ggcgacgcag cagttcgagg tcagcgatac gcgttcgccc gaggaggtgg ccgccgcgct
      661 gcgcgcgatg ggctacgacc cggtgtggaa ggactgggac gccggccacc gcgcgcccgc
      721 ggaggcggcg agatgacgat caccgcgaac ggcaagccgc gcgagatccc ggacggcatc
      781 acgatccgtg ccttcctcga cacgctcggc tggaagcccg ggtgggtggt cgtcgagcac
      841 aacggcgagc cactcgaccg cgcacgctat gacgccgtgg cgctgcgcga cggcgaccgc
      901 atcgaggtcg tgcgcgccgt cgcgggaggc gcgcgatgac cgcacgccgc gagcgtctcg
      961 atgccgcgcg gctctatctg gtctgcggcg ggcagcggcc ggacctcgac gagttcctgg
     1021 aggccgcgct gacggcgggc gtggacgtcg tgcagcttcg cgagaagcag atggaggcgc
     1081 ggccgctgct cgagatcgcg gcgcgctttc gtcgggcctg cgatcgtcac ggcgcgctgc
     1141 tcgtgatcaa cgaccgtccc gacctggcga tcgcgtcgaa cgccgacggc gtgcacctcg
     1201 ggcaggacga tctgccgccg gccgcggggc gtgcgctcgt cggcaacgag atgttgatcg
     1261 gccgctcgac gcacgcaccc gacgagacgc gtgccgcgct gagcgagccc gtggactaca
     1321 tctgtgtcgg acccgtgcac gccacgccca ccaagcccgg ccggtccgcg accgggctgg
     1381 atctggtgcg cttggccgcc gcggaatgcg ccaagccctg gttcgcgatc ggcgggctcg
     1441 acccgcagac cgcgaaagag gcggcgcagg cgggcgcgac ccgcatcgtg gtcgtgcgcg
     1501 caatcgagca ggccgccgac gtcgaaggcg ccgtgcgcgc cctgcgaagc gcgatcggcg
     1561 cctgaaccgc tcctgcacgc ttgcttcgcg tccgggctat agcatcggcg ccgaagactc
     1621 cgctcgaaag gagaagccga tgaagcgcct catggccgcg atcgtgctcg tgctcgcgct
     1681 cggtgcgtgc gaggcccgcg ccgagatcga cgtcaacgac aacggctcgg gcacgttcgg
     1741 cttcgccttc ttcatcgaca agcagttcat cgcgttgatg gagggcttcg gcgggcggca
     1801 gggcgaggat ccgttcggcg acttcaagag cggcctcgac gattcccccg tgccgtttca
     1861 gatcgaggag tacagcgaga acggcggccg cggaatccgc gccaaggttc ccttcaagag
     1921 cgtggacgat ctgcgcacga tcatgaaaga ggtggaccaa ggcgacgccc aaggctcccc
     1981 gctcggcgga ggcgggccgg gcttcggcac gttcacgctc gagcagcggg gcggcggctg
     2041 gtacttcgag tcgctcagcg acgcgccgga tctgggcgat ctcggggatc ttggctcagg
     2101 tgaaggcggg ggcgaagccc cgttcgatcc cgcgcagatc gcgagcctgc tgaaaatctc
     2161 gttccgggtg acgcttcccg gccggacggt ggacacgacc gcggacgagg ccacttccaa
     2221 gggcggaaag acgacgttcg tgtggaacgc ggacctgacg agcaaagagc cgatgcagat
     2281 ggtcgcgacg accgctgcgg ccggcagcag cctcccgttg tcgccgatcc tgatcgcctt
     2341 ggccctgatc gccgcgctcg tgaccgtcgc ggtgatgatg cggcgcaaga acgcggtcgc
     2401 ctcaggtccc gatcagcacg cggatgcgtg gccttcgggg acgggtggcg cggtcccgcc
     2461 tcagccgggc accgtgcctc ccgtttcggg cacccacgag caggatcccc agcgcccact
     2521 cgagccgccg gcgccgtaga ccgcgcaccc tgcttgcgtt acgggcgcga ccccgccatg
     2581 atgcgctccg tagaccttcg caaaggagtc ccgttgaagc ctgcttctat cgtcactgcc
     2641 ggagtcgcgc tgtcgctgtc gctgatcgcc tgtggcggga gcgacggccg cgtcgccgcg
     2701 cccgcgttta ccccgaccga acagcagacc gccggcgacg atcctgtttc cgccaagccc
     2761 gcgtgcgact acgtgaccga ggccgaagcg agccaggtct tcgagaccga gatgaagtat
     2821 cgctccaacg acggcgggac caactgcatc ttcgacgggt cgccggatgg ctcggccagc
     2881 atcgactaca cgatcagcga cggcaccgac ggctatgaca tcgtctcgga cggcatgcag
     2941 ccggtcgacg ggatcggaga ccgtgcggcc gggcgcgacg gttccgggct gtcccaagtg
     3001 gtcttcacca agggcaacac gatggtcacg ctgacggtga gcggtaacga catcaaggga
     3061 ggcggaccct tcgatgtcgc aaagaagttc gcactgttcc tggccggcaa gctgtaggcc
     3121 ctagcgcacc gagtcgagcg cctgcttcag atcggcgacc agatcgcgcg cgttttcgat
     3181 tccgacgctc agccgaacca gcgacgcatc gacctccagc ggcgagcccg cgaccgacgc
     3241 gtgcgtcatc ttgccgggat gctcgatcag cgactcgacc gcgccgagcg attcggcgag
     3301 cgtgaagatc ttcgtgcgcg ccacgatgtc cagcgcggcc tgctcgccgc ccgcggccag
     3361 gaacgacacc atgccgccaa agtcgcgcat ctgcttggcg gcgatctcgt ggccgggatg
     3421 ggtgggcacg cccgggtaca gcaccgtcgt gacggcggga tgctgctgca gcatcgcgac
     3481 gatctcgcgc gcgttcgcgc attggcgttc gacgcggatc gcgagtgttt tcacgccgcg
     3541 cagcaccagg tagcagtcca gcggccccgg caccgcgccg accgcgttct gcaagaactt
     3601 cagccgttcg aacgtctcgt cgtcgtcggt ggcaaggaag ccgccgacca cgtcggagtg
     3661 gccgccgaga tacttggtcg cgctgtgcag cacgatgtcg gcgccgagcg acagcggctg
     3721 ctgcaggtac ggcgtcgcga acgtgttgtc cacgatcagc cgcgcgccgc gatcacgcgt
     3781 gatcgcggcg acggccgcga tgtccacgat cttcagcagc ggattcgtcg gcgtctccat
     3841 cacgaccagc ttcgtgttcg gccgccaggc cgcttcgatc gcggcggggt cgcccatatt
     3901 cacgggcgtg aactcgtagc cctgcggtgc gaagacctgc gcgaccagcc ggtaggtgcc
     3961 gccgtaggca tcgtcgccga tgatcagatg atcgcccggg ttgatcagcc gcagcgccgc
     4021 atcggtcgcg gccaggccgc tggcgaagca caagccgtgc gccgcgcctt ccaacgatgc
     4081 gaggcatgcc tcgagcgccg cccgggtcgg gttacccgag cgagcatagt cgtagccctt
     4141 gtgctcgccg accgcgctct gcgcgaacgt ggtcgagaag gaaaccggcg ggatcaccgc
     4201 gccggaggca ggatcgggat cttggccggt gtgaatcgcg cgcgtctcga actcgtagtc
     4261 gctcatcgcc cgagattcta gcggcgcggc ctcattcgcg gggcggggcg cggccgggca
     4321 gcggactgtg gtagcgggct ccccgtcgtg cgaagtaaag gctgccggtg gtatcgcgcg
     4381 tgatcgtcca gccgcggcgg tggacgcgat ggtggcatcg gcggcagagc atgaccaggt
     4441 tgtccgggtc ggcggtcccg ccgtcgcgtc gcgcgcgaat gtgatgcgcg tccgcccacg
     4501 acaggggcgc gttgcagccg gggaaccggc agccgcggtc gcgggctgcg acagcgcgcc
     4561 ggatcttcgg agggatcggc gaggttgcat cgcccaccgc gaccggcgtg ccgcgttcga
     4621 aaaggatcgg aagcacgtcg gcatcgcagg ccagcgtctc ggtacccagc ggcgacaggc
     4681 gcggcgcccg gccggcgatt gcccacagga tgcgggccga agcttcccgc cggtcgcgag
     4741 tcaggtcggc gacatcgatc gtcgccagca gccgcggacg cgaccgcgtc ggcagattgc
     4801 tgccgctgct gcccccaagg tgctgctcgg cgatcgcgat cagcgcgtcg tagcgccgct
     4861 gcgcgagcgt gtcggccggt gacggagcgg ctgcggccgt gtcggccgct tcgatcagcg
     4921 tcgcgaccga gaccgtgtcg ccctcgccgt agaagaacgt gcggccgtcg agtcgcggct
     4981 ggaacgtgac gaagctgcga tcgacggcct gttgctcgtg ccgttcttcc cggcggtgtt
     5041 tcagctcggc cacgaggtcc tcgacgtcgc gtacgatctg atccggatcg gcgtcgcgca
     5101 ggaagagcgc gtcgcgtgcg acgagccggt cgaccgcgcg ccggccggcc gcatcgcatg
     5161 atcgcgccgc attgcagatg ccccgaacct gagaccagga cagcagcccc tcttgaaacg
     5221 cgtgggcaga gttgggcatg gaatcgagca tcgcggcgct ccgcgtgagt gcgcgcgcat
     5281 cccacgacgt tgcccgcgcg tccatctgga gcatgatcgg agcgggaacc ccggccccgg
     5341 acgcgaagcc gccgcgcgag atcgtggtcg ctgccgcggc ggtcaaccga tccgccgcgc
     5401 gtaacgctgc gaccgccaag ctcgcgcgcc gctcgccgcc gcgagaggcg aaccgttgaa
     5461 cccgatcgat aatcgccccg agctctgccg tgagctgatc gatctcggaa tctgccaccg
     5521 aaagcatgcg acgaacatac ccgagaggta cgacagaaac gctctcccac ttgccgcgtt
     5581 tcgacagaaa accccggcgc acggtctcgt cgggatggat ggggagggtc gcggtaacag
     5641 gtagggtgtc gccttatcgt acgacgcgtt cgagtccgtt tccgaccagg atcatgctga
     5701 gtgcgaagag cacggtcatt gaaccggcca gccatcgtct tttccgcggc cacacgtaga
     5761 cgacagccgc aatcgctgcg acggccagtt gcatccccaa agctgcgtac ggccacccga
     5821 tgtagtcgat caatgcgcag tgcctgtaga cgcaccaggt cccaagacca tcaacatagg
     5881 gcagcgcgtc gggatgacca ttgtagagac cgaacgagta ggcgggagcc gcgatggcgg
     5941 ctgctatcca cagactcgac acgatcaccg cgatgggcgc tgatacccgt ctgaggcgaa
     6001 accagacgac aaggctgagg cttgccgcca gcatcaggaa gatgaccctt tcaatgagcg
     6061 tcatccgaaa cccggcgggc aagacgagtc caactcctgc acgggcgagc gtgagccatc
     6121 cggggaggcg accatagaac gacgatattc cgggtcccat gaagtagaga aggcttccgg
     6181 cgagcgttac cccggcggag catgcaccca caatcaggaa tggcactcgc agccgatcaa
     6241 cagcgacgcg tcgggatgaa gtagccatcg gctctccgcc tagcatccgc cgcaccacct
     6301 ggggaggaat cccgcgtcgt ctcaggagac ggacccgctc gtggttgagc atcgttcggg
     6361 gttgttcttt ggatcgggcg catcgacgtt gggatccttt cgtggaggca cgcgagcgct
     6421 cgtgcgactc gtcgagctgg gaagggcgcc ccgaatgcgg gccgcatcga acagaccaac
     6481 gactagggca tggtcgcgtt aacaggtaag gtgtcgtctt atcccacaac gcgtttgagt
     6541 ccgtttccga ccaggatcat gctgagtgcg aacagcacgg tcattgaacc tacgcgcagc
     6601 catcgtcttt ccttccgcca ggcgtggacg gcagccgcga tcgccacgac ggccagttgc
     6661 atccccaaag ccgcgtacgg ccagcgggtc cacaccacgg gtatcatgct cgaaccggcg
     6721 attccataat tcgtcccaaa gttctctaga gggccgtcgt acaagttgaa ggagtaggtg
     6781 ggagccgcga tggcggctgc tatccagagg ccggacacga ccctcgcgat ggccgctggc
     6841 acccg
//
LOCUS       DATLFO010000339         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_39346, whole genome shotgun sequence.
ACCESSION   DATLFO010000339 DATLFO010000000
VERSION     DATLFO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39346"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1010)
                     /locus_tag="VM841_13655"
     CDS             complement(192..1010)
                     /locus_tag="VM841_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015108491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVL91274.1"
                     /translation="MRVAVVGHTEWIDFVRVSHLPAPGEILHAEDAWEEPGGGGAVAA
                     GQLARLAGECVFVTAFGEGPHGPATRAALQTMGVRVEAALRPTPHRRAITMVTPAAER
                     TILVLGDRLGPEAADPIGWDLLDAADACYFTAGDVHALHRARRARVLVATPRAMETLQ
                     GSGVRLDALVGSAADQGERFDDFDLDPPPRLLVRTEGAKGGMYGPPGEKPRRFAAAEP
                     PGPPVDAYGCGDSFAAGLTFALGKGMDDDDAVAFAARCGAACLTGRGPYAGQLR"
     gene            complement(1015..1725)
                     /locus_tag="VM841_13660"
     CDS             complement(1015..1725)
                     /locus_tag="VM841_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91275.1"
                     /translation="MGETAWTGTPVIPHPDARDNDAEKLPWGGRREKLPMRWPFADAV
                     EGFRSKALANKQYDPASTFVWGQMMAVGLIEMLKAIEAAFGADGHDVARAALRKVGDR
                     IATEMIDGVEKPGDLSPAELSSLFASWINEVAYASIENPKVEGDGASFDIHYCPHEDV
                     YGAFDCRVQRYLVEGMIEAARRHWGDGMIDVAFATTIPSGSRTCHFDMFPKGEGSDKW
                     FEYSDRLRDRALKIVDVK"
     gene            1857..>2196
                     /locus_tag="VM841_13665"
     CDS             1857..>2196
                     /locus_tag="VM841_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91276.1"
                     /translation="MATSTLDELRAAVRGTVIGAGDPGYDEARRVYNGMIDRKPQAVI
                     RCAGTEDVVAAVNYARENGLDLSIRGGGHSVPGFGTNDGGIVIDLSQMTAVKVDPQAR
                     TARAQGGATWG"
BASE COUNT          346 a          762 c          760 g          328 t
ORIGIN      
        1 gggaggagtg gcgcacaatg gactcgtcaa gaaatttgac ggcgtcaaga aacttgacga
       61 gtgctctgtc ccacacaccc ccgggcgtcc tgtgtgcgtg aggactgctc tcggcccccg
      121 gtgtgctgcc gcatgcgccg cacgggggag ggcctgggaa tggccggcga cgtaacggtc
      181 cgcgactctt gctagcggag ctggccggcg taggggccgc ggccggtgag gcaggcagct
      241 ccgcatcgtg cggcgaacgc gacggcgtcg tcgtcgtcca tgcctttgcc gagcgcgaag
      301 gtcaagccgg cggcgaagct gtcgccgcag ccgtaggcat ctacgggcgg gccgggcggc
      361 tcggcggccg cgaaccgccg gggtttctcg ccgggcggac cgtacatgcc gcccttggcc
      421 ccttcggtgc gcacgagcag ccgcggaggc ggatcaagat cgaagtcgtc gaagcgctct
      481 ccttggtcgg cggcgctgcc gacgagcgcg tccagccgca cgccgctgcc ttggagcgtc
      541 tccatcgcgc gcggcgtggc gacgagcacg cgggcgcggc gcgcgcgatg cagcgcgtgc
      601 acgtcgccgg ccgtgaagta gcacgcatcc gcggcgtcga gcaggtccca gccgatcggg
      661 tcggccgcct cgggaccgag ccggtcaccg agcaccaaga tcgtacgctc ggccgcgggc
      721 gtgaccatgg tgatcgcgcg gcggtgcggc gtgggacgca gcgccgcctc gacgcgcaca
      781 cccatcgtct gcagcgccgc acgcgtcgcc gggccgtgcg gcccttcacc gaacgcggtg
      841 acgaacacgc attcgccggc aagccgcgca agctgaccgg ccgcgaccgc tcctccaccg
      901 ccgggctcct cccatgcatc ctcggcgtgc aggatctcgc cgggtgccgg caggtgcgac
      961 acccgaacga agtcgatcca ctcggtatgc ccgacgaccg cgacccgcac cgcgctactt
     1021 cacgtccacg atcttgagcg cgcggtcacg cagccggtcg gaatattcga accacttgtc
     1081 gctgccttcg cccttcggga acatgtcgaa gtgacaggtg cgcgagcccg acgggatcgt
     1141 cgtcgcgaat gcaacatcga tcatgccgtc gccccagtgg cgccgcgcgg cctcgatcat
     1201 tccctcgacc agataccgct gcacgcggca gtcgaacgcg ccgtagacgt cctcatgcgg
     1261 acagtagtgg atgtcgaacg acgcgccgtc gccttcgacc ttcgggttct cgatcgaggc
     1321 gtaggcgacc tcgttgatcc acgacgcgaa caggctcgac agctcggccg gtgacaggtc
     1381 gccgggcttt tcgacgccgt cgatcatctc ggtcgcgatt cgatcgccca ctttgcgcag
     1441 cgccgcgcgc gccacgtcgt ggccgtcggc gccgaacgca gcctcgatgg ccttgagcat
     1501 ctcgatcaga ccgacggcca tcatctggcc ccacacgaac gtcgaggccg gatcgtactg
     1561 cttgttggcc agcgccttcg agcggaagcc ttcgacggca tcggcgaaag gccagcgcat
     1621 cggcagcttt tcgcgccggc cgccccacgg gagcttctcg gcgtcgttgt cgcgcgcgtc
     1681 gggatgcggg atcacgggtg ttccggtcca agcggtttcg cccattgcct tgctcctggt
     1741 cggttcatct agggggttga ctaccgcgtt cgataggaga agcatgaccc ggaagaccca
     1801 tcgccgtcca gcgacgcctg tcgccgggcg gtgacgacga gcgaaggggg caccatatgg
     1861 ccaccagcac tctcgacgaa ctccgcgccg cggtacgggg aacggtgatc ggcgccggcg
     1921 atcccggcta cgacgaggcc cgccgcgtct acaacgggat gatcgaccgc aagccgcagg
     1981 cggtgatccg atgcgccggc accgaggacg tggtcgccgc cgtgaactac gcgcgcgaga
     2041 acggcttgga cttgtcgatt cgcggcggcg gacacagcgt tcccgggttc ggaaccaacg
     2101 acggcggcat cgtgatcgac ctatcgcaga tgacggccgt gaaggtagac ccgcaggcgc
     2161 gaaccgcccg cgcgcagggc ggcgcaacgt ggggca
//
LOCUS       DATLFO010000340        10537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_17956, whole genome shotgun sequence.
ACCESSION   DATLFO010000340 DATLFO010000000
VERSION     DATLFO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17956"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VM841_13670"
     CDS             complement(<1..184)
                     /locus_tag="VM841_13670"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HVL91277.1"
                     /translation="MSQDATELWSRAVSRMRDRLPSPGARPWIEGTRAIGLTGTTMTL
                     AAPSAFAKEWLETRYSD"
     gene            478..621
                     /gene="rpmH"
                     /locus_tag="VM841_13675"
     CDS             478..621
                     /gene="rpmH"
                     /locus_tag="VM841_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HVL91278.1"
                     /translation="MAKGKRTFQPHNRRRKRKHGFRARMRTKAGRLIIARRRRKGRAR
                     LTA"
     gene            639..986
                     /gene="rnpA"
                     /locus_tag="VM841_13680"
     CDS             639..986
                     /gene="rnpA"
                     /locus_tag="VM841_13680"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HVL91279.1"
                     /translation="MQRRNRLGSESLIRRVYATGVRVRSKGATVTGIVEGDSAPRVAI
                     VASRRVGGAVVRNRARRRLRAALGGALPFMKPGSVAVVAATPETPSLDFQILEDSVRR
                     GLVKAGLVGAAGA"
     gene            979..1263
                     /gene="yidD"
                     /locus_tag="VM841_13685"
     CDS             979..1263
                     /gene="yidD"
                     /locus_tag="VM841_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HVL91280.1"
                     /translation="MRNPVAVAVIVLIKGYQATLGKIFGGSCRFHPSCSDYALKAVQL
                     NGALVGSGQAVWRVVRCAPWSKGGVDYPKPTRRAKMRAHAAEHRTEAARG"
     gene            1295..2443
                     /locus_tag="VM841_13690"
                     /pseudo
     CDS             1295..2443
                     /locus_tag="VM841_13690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
     gene            2440..3147
                     /gene="jag"
                     /locus_tag="VM841_13695"
     CDS             2440..3147
                     /gene="jag"
                     /locus_tag="VM841_13695"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HVL91281.1"
                     /translation="MSDSVETTGASVEEAIERALDELGAREDEVEIEVLPDPDSDDPA
                     KRGPAKVRVRFRDERSAEEHAAEKIEEAEERDRRRTISDEEAQRQVEATHTFLRGLLD
                     VMELDDVDVEVTATAFGAEAEIRGEDLAILIGRHGATLGALQDVTRAAVQQATGVRAA
                     ITVDIEGYFERRAEKLEQLARAAADKVSRSGKPYKMEPMAARERKIVHDALTAIGGVR
                     TASEGEEPYRCVVVLPL"
     gene            3223..3825
                     /gene="rsmG"
                     /locus_tag="VM841_13700"
     CDS             3223..3825
                     /gene="rsmG"
                     /locus_tag="VM841_13700"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HVL91282.1"
                     /translation="MRDDIGIAALSTVLRRLRLPATLLSPLLAHADAVRAASPRLGLV
                     STGDEDSILMRHTADSLLFALAYPPVGGERWVDVGSGAGFPGMCLAICYPQAGFSLVD
                     ANRKKAGFLDMQVLDLGLANVRVFAERAERLETDFDVAVARAVTDPAEATRRLLTMVK
                     PGGKVIVAGTGTVVGAEVVQPDIPEVDSPRRLFMMTRTIE"
     gene            3830..4708
                     /locus_tag="VM841_13705"
     CDS             3830..4708
                     /locus_tag="VM841_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL91283.1"
                     /translation="MDSSNFQPSAPEGRALALALANQKGGVGKTTTAVNLGACLAEAG
                     ARILLVDADPQGNASTGLGIDRSRIKTGTYEMLLGAPVSEAVVETPVPNLSLLPSTID
                     LAGAEVELVSTFAREGKLRNAIEPVRGSYDLIVIDSPPSLGLLTINALVAADQVVVPI
                     QCEYYALEGLGQLMRTIDLVRDALNTNLTIGGVVLTMFDSRTRLAEQVVADVRAHFGD
                     VVFDTVIPRSVRLSEAPGFGKPIIQYDAAGKGAAAYRSLAAEAATRWLRAPAAVAGGA
                     DDASPAGDAHAEGDSR"
     gene            4705..5616
                     /locus_tag="VM841_13710"
     CDS             4705..5616
                     /locus_tag="VM841_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVL91284.1"
                     /translation="MSDETTPERRPGGLGRGLSALLPSSPSGQPSSPQAPGSAGPSLR
                     EIPIDEIAPNPAQPRDVFDHDKLQELADSIRSVGILQPVVVRRHGAGYQLVMGERRVR
                     AARLAGLRMIPALVRDTADDAMLRDALIENVQRQDLNPIEEAIAIKALVEEHAITHDE
                     VAERLGRSRPAVTNALRLLTLSDAVRERVAAGVLSAAHGRALAAIADHETQERLARRI
                     SAEGMSVRATEDAVRRLTAAGAPAGRPAKVPASRSAGILEVEHRLADMLDTRVRVEAA
                     RRGRGRIVVEFADEADLDRIFRSIARD"
     gene            5660..6829
                     /locus_tag="VM841_13715"
     CDS             5660..6829
                     /locus_tag="VM841_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVL91285.1"
                     /translation="MEFRRIDRLPPYVFSVVNDLKAAARRAGEDIIDLGMGNPDLATP
                     GVVVEKLTEAARNPRNHRYSTSRGVPKLRKAICDWYERQYGVTLDPETEATSVMGAKE
                     GLSHLAWTLVQPGDTVLVPEPTYPIHTFSFALAGADVHAVPMSAGADLFEGLLDAYER
                     CWPRPRVLLTSFPHNPTTACVDSAFFERLVAFAQEHSLIVVHDFAYADLCFDGYEAPS
                     FLSVPGAKDVGVEIFSMSKSFSMAGWRIAFMVGNPEIVAALTRLKSYLDYGVFQPIQI
                     AAIIALNECGNETKVFNDVYRTRRDALIEGLQRAGWDVPPPQATMFVWAEIPDAHKAM
                     GSLEFAKFLLAEGKVAVSPGVGFGRAGDGHVRFALVENEHRIRQATRGIRKALGG"
     gene            6832..6960
                     /locus_tag="VM841_13720"
     CDS             6832..6960
                     /locus_tag="VM841_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91286.1"
                     /translation="MADVVVDINEGKESEWQRGLAGFLLGLALGALVALLSKRKPG"
     gene            6957..7574
                     /locus_tag="VM841_13725"
     CDS             6957..7574
                     /locus_tag="VM841_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91287.1"
                     /translation="MTRRVEDIDVRTVRDLPHRCRGCVFWELSPADRAFAVEHDAEFE
                     KEAWCSELSLTWGAPGKIVYVDDEPAGFALAGPPTAFPRAGFFPAKVSDDALLLATMH
                     VLQSHQGRGIGKVLIQNVLKMAKEHGLRAVECFADKQWAGYDCLIPAEFCHAIGFRIK
                     RDHVRFPLMRIDIKALAKLAESFEAKLESFLESLKVPSTAPRPVR"
     gene            complement(7689..8762)
                     /locus_tag="VM841_13730"
     CDS             complement(7689..8762)
                     /locus_tag="VM841_13730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVL91288.1"
                     /translation="MLYTEGSAGEAVRDIQRRLLAFGASIDESERARRRFGPSTTAAV
                     RAFQQRRGLISDGIVGPQTWRELVEASWNLGDRVLYVRSPQMRGDDVRELQQRLSKLG
                     FDPGRIDGILGPQTIRVVVEFQRNYGLPPDGIIGASTIRALIGMPSLGGDTPVTRLRE
                     REHLRRLPPTLAGLTIVVDPGHGPADPGHSGPGGLHECDVAFAIARRLEAALAASGVA
                     VFLTRDDASTPGEAERGALANTLGADLYVAIHMGGSADPHASGAAAFYFGTDVWESDG
                     GRRLAETIMDHLAGLGLADGRSHAKTWGVLRETRMPAVQVEPCYITNPDEAARLTEPE
                     FQRRIAEAVFAAIRTFAVSPIPA"
     gene            complement(8813..9145)
                     /gene="trxA"
                     /locus_tag="VM841_13735"
     CDS             complement(8813..9145)
                     /gene="trxA"
                     /locus_tag="VM841_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVL91289.1"
                     /translation="MVAGPTITVTDSDFEDKVLKSSTPVLVDFWAEWCGPCHMVAPVL
                     EEIAGEMQGKLTVAKLDVDNNPDVSRRYGILSIPSMLLFVDGVEKRRVVGARGKQQIL
                     GELSEFVS"
     gene            complement(9266..10402)
                     /locus_tag="VM841_13740"
     CDS             complement(9266..10402)
                     /locus_tag="VM841_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVL91290.1"
                     /translation="MKIERIDAWICHFPLAAPFSPSWVPGLANPNNSCVIYRLVTDDG
                     VEGYTGWIAFADEGRGPINLLRAFLIGRDPTRIAEFRPILETAVRVLGLRVWFVEAAL
                     YDLAAKAAGVPLYRYLGGTRDRVPVYASFGEVRDPKQRADDALAAMERGFKAVKLRPR
                     HDTMREDVAEVRAVREAAGDDLTIACDANQGWRVDVFKGDSPRWDFKRALATAKAYEE
                     FDVAWLEEPLDQFDFEGYRRLRDQTTTPIAGGELAGDIWPLREHIERRSLDIIQPDAM
                     FTGGITGALEIATMAKNAGIGFNPHTWSNGLGFAANLHVFAATHGAWLEYPYDPPGWT
                     PEGRDAMLTEPLVTDEDGRLSLPQAPGLGVELDIEKIQACGLAL"
BASE COUNT         1693 a         3553 c         3614 g         1677 t
ORIGIN      
        1 gatccgagta gcgggtctcg agccattcct tggcgaaggc gctcggcgcc gcaagcgtca
       61 tggtggttcc ggtcaggccg atcgcgcggg ttccctcgat ccacggccgg gctcccggag
      121 agggaagccg gtcgcgcata cgggagaccg cgcggctcca aagctcggtt gcgtcttgac
      181 tcacgttgtc ctctcccgtc ctgtccacag gctccatcca caagatgtgg acggatgaac
      241 cgatgtgttc agcgcacggc agagcgagag gggaggtggg aagagctctg cgtgaggggc
      301 ggagtatagg cacggcctct cgagccttca agatggggaa aacaccttgt ggacaactgt
      361 tttcgcaggt aaacgaaggt ttttcgggcc ggaacgatct tcggcgggcg agccggcgct
      421 tgttccggcc ccgtgttgac gctgcgaagg tctcgcccgt acactgcctc ggttctcatg
      481 gctaaaggca aacgcacgtt ccagccccac aaccggcgcc gcaagcgcaa gcacggtttc
      541 cgtgcgcgca tgcgtacgaa ggccgggcgt ctcatcattg cccggcgccg ccggaagggt
      601 cgcgcacgcc tgacggcttg acgccctacc ctccttcgat gcagcgacgc aatcgtctcg
      661 gctccgagag cctgatccgg cgtgtctacg cgaccggagt tcgcgtgcgt tcgaaggggg
      721 ccaccgtgac cggaatcgtc gaaggagaca gcgcgccgcg cgtcgcgatc gtggcatcca
      781 ggcgggtggg cggcgccgtc gtccggaacc gcgcccgccg ccggcttcgt gccgcgctcg
      841 gcggcgccct tccttttatg aagccgggat ccgttgctgt ggtggccgcg acacccgaga
      901 ccccgtcgct agacttccag atattggaag atagcgttcg gaggggcttg gtcaaggccg
      961 gactcgtggg ggcggccggt gcgtaatccc gtcgcagttg ccgtgatagt cctgataaag
     1021 gggtatcaag cgacactcgg caagatcttc ggcggctcct gccgctttca tccgtcctgc
     1081 tccgactatg cgctgaaagc cgtgcagttg aacggcgcgc tcgtcggatc gggccaggcc
     1141 gtctggcgcg tcgtgcgctg cgcgccgtgg tccaagggcg gcgtcgacta tccgaagccc
     1201 accaggcgcg cgaagatgcg tgcccatgcg gccgaacatc gaacggaggc cgcacgtggc
     1261 tgaagcattc ggagggatcc tcaggttttt cggactgatc ctcaacgcta tctacgaagt
     1321 catcccgtcc tacggcgtcg cgatcatcct gctcacgatc gtggtccgga tcgcgctgtt
     1381 cccgctggcc gtgaaacaga ttcgctcgat gacggccatg cagaagatcc agcccaagct
     1441 caaggagatc cagaagaagt acaagggcga ccgccagaag ctcaacgaag agatgatgaa
     1501 gctctaccgc gagcacggcg tcaatccgct cggcggctgt ttcccgatgc tgctccagct
     1561 tcccgtgttc atcgcgctgt acgccgtgat cagggccagc atccccttcg acgccgcggt
     1621 cgagccggcg cagccggtca acagcgagtt ccgggtcacg aagcagacca tctgccggcc
     1681 ggacggcgag ccgaccgcga agaacggcgc cgacttcagc accatcgtgt gcgacaccga
     1741 aggacaggag cagtccttca ccgtcgccgg cccggtcctc gagggcaaga agacgcagca
     1801 actagatgcc ttcccgtcct acgtggtgcg atgcgacacg cggaccgaag gcgagggcga
     1861 ggaccagcgc gtcgtcggca tgcgttgcat ctcgccgctg ggaaccggcc atctgcctcg
     1921 agacggagcg ctgttcgcgg cgctggtgga tgacggcgag ccgttcctcg ggatacagct
     1981 tgcctgcacc ccgactcagg ccaactcaaa gaaggctgcc cgtgcgtgcg caggcggaga
     2041 gggctacggc ggcggaccgg cgctgatcgg ctactacctg ctggtcctgt tgatggcggg
     2101 caccaccttc taccagcaaa aacagctgct ggcgcgcaat cagggcaccg cgcaggccgc
     2161 ccagatgcag atgatggcca agatcatgcc tgtctttatg ggcttcctgt cgctcaactt
     2221 cccggcggcg ctcagcttgt actgggtggt cggtaacttc tggatgatcg gccagcagca
     2281 cttcatcctg gccgcgcagg acaaggccga tgcgccggcg ccgggggctc cgccgccggt
     2341 cgtgaccaag cgtgagaagc ccaagccgat gacacccggc aacggccgtc ccggaggcaa
     2401 gtcgggcaag aagccgcgcg gcaagtccgg caagcgccga tgagcgactc ggtcgagacc
     2461 accggcgcgt ccgtcgaaga agccatcgag cgggcgctag acgagctcgg cgctcgcgag
     2521 gacgaggtcg agatcgaggt cctgccggat cccgacagcg acgatcccgc aaagcggggg
     2581 cctgcgaaag tgcgggtgcg cttccgggac gaacgctcgg ccgaggagca cgcagctgag
     2641 aagatcgaag aggccgaaga gcgcgatcgc cgccgaacga tctcggacga ggaggcgcag
     2701 cgtcaggtcg aggccaccca cacgttcctg cggggcctgc tcgacgtcat ggagctcgac
     2761 gacgtggatg tcgaggtcac ggccaccgcg ttcggcgccg aggccgagat ccgcggcgaa
     2821 gacctcgcga tcctgatcgg gcgccacggc gcgaccctcg gcgcgctcca agacgtgacc
     2881 agggccgcgg tccagcaggc aaccggagtg cgcgctgcga tcaccgtcga tatcgagggc
     2941 tacttcgagc gacgggccga aaagctcgag cagcttgccc gcgcggcggc cgacaaggtc
     3001 tctcgcagcg gaaagcccta caagatggag ccgatggcgg cccgcgagcg gaagatcgtt
     3061 cacgacgccc tcaccgcgat cggcggcgtg cgcacagcca gcgagggtga ggagccctac
     3121 cgctgcgttg tcgtcctccc cctgtaagcc ctcgccgttt cacgtgaaac ctcgccttcc
     3181 gcgacttggt ggggaccccg tggcactgtt tcacgtgaaa ccatgagaga cgacatcggt
     3241 atcgcagccc tctccacggt tcttcgtcgt ctcaggctcc ccgccacact gctctcgccg
     3301 cttcttgctc atgcagatgc cgttcgtgcg gcgtccccgc gcctcggtct ggtctcaacg
     3361 ggcgacgagg attcgatcct catgaggcat acggccgatt ccttgctgtt cgcgctggcc
     3421 taccctcccg tggggggcga gcgctgggtc gacgtcggat ccggggcggg ctttcctggg
     3481 atgtgcctcg cgatctgcta tccccaggct gggtttagcc ttgtggacgc aaatcgaaag
     3541 aaggcgggat ttctggatat gcaggtccta gatctggggc tcgctaacgt ccgggtcttc
     3601 gcagaacgag cggagaggct tgaaacggac ttcgatgtgg ccgtcgctcg agcggtcacc
     3661 gacccggccg aggccactcg caggctgctg acgatggtga agcccggcgg gaaggtcatc
     3721 gtcgccggaa ccgggaccgt cgtgggagcc gaggtcgttc agcccgatat tcccgaagtt
     3781 gactcgcctc gtcggctctt tatgatgacg cgaaccatcg aataacccca tggattccag
     3841 taatttccag ccaagcgcgc ccgaggggcg tgcgcttgcg cttgccttgg ccaatcagaa
     3901 aggcggcgtc ggcaagacga ccaccgcggt gaacctgggg gcatgcctgg ccgaggccgg
     3961 ggcgcgcatc ctgctcgtgg acgccgaccc gcaaggcaac gcctccaccg gcctgggcat
     4021 cgaccgctcg cggatcaaga ccggcaccta cgagatgctg ctcggcgcgc cggtctccga
     4081 ggcggtcgtc gagacgccgg tgccgaacct gtcgctgctg ccctccacca tcgacctggc
     4141 cggcgccgag gtcgagctcg tctcgacctt cgcccgcgaa ggcaagctgc gcaacgcgat
     4201 cgagccggtg cgcggctcct acgacctcat cgtcatagac tcgccaccgt cgctcggtct
     4261 gctcaccatc aatgcactcg tcgcggccga tcaggtcgtc gttccgatcc agtgcgaata
     4321 ttacgcgctg gaagggctcg ggcagctcat gcgcaccatc gatctggtcc gcgatgcatt
     4381 gaacaccaac ttgacaatcg gcggtgtcgt tctgaccatg ttcgactcgc ggacccggct
     4441 ggctgagcag gtcgtggccg acgtgcgggc ccacttcggc gacgtggtct tcgacacggt
     4501 catcccccgc tcggtgcgcc tgtcggaggc ccccggcttc ggcaagccga tcatccagta
     4561 cgacgcggcc ggcaagggtg ccgccgcata ccgctccctg gccgccgagg ccgcgacccg
     4621 ctggctgcgt gcgccggcgg ccgttgcggg cggggccgac gacgcgtccc cggccgggga
     4681 cgcgcatgcc gaaggagaca gccgatgagc gacgagacca cgcccgaacg gcggcccggc
     4741 ggcctcggcc gcggcctgtc cgccttgctg ccgtcctcgc cgtccgggca gccgtcgtcg
     4801 ccgcaggcgc cgggatcggc aggcccgagc ctgcgcgaga tcccgatcga cgagatcgcg
     4861 cccaaccccg cgcagccacg cgacgtcttc gaccacgaca agctgcaaga gctcgccgac
     4921 tcgatccgca gtgtcggaat cctgcagccc gtggtggttc gccgccacgg cgccggatac
     4981 cagctcgtca tgggcgagcg ccgtgtgcgc gccgcccggc tcgcgggctt acgcatgatc
     5041 ccggcgttgg tgcgcgacac cgccgacgac gcgatgctgc gagacgcgct gatcgagaac
     5101 gtccagcgcc aggacctgaa tccgatcgaa gaagccatcg cgattaaggc gctcgtcgag
     5161 gagcacgcca tcacccacga cgaggtggcc gagcggctcg gccgcagccg gcccgccgta
     5221 acgaacgcgc ttcgcctgct cacgctctcg gacgcggtcc gcgagcgcgt ggcggccggt
     5281 gttttgtccg cggcgcacgg ccgggccctc gccgcgatcg ccgatcatga gacccaggag
     5341 cggctcgccc ggcggatctc cgccgaaggc atgagcgtgc gcgcgaccga agacgccgtc
     5401 cgccgcctca ccgccgccgg cgccccggcc ggcagaccgg ccaaggttcc cgcgtcgcgc
     5461 tcggccggaa tcctggaagt ggagcatcgg cttgccgata tgctggatac gcgcgtgcgc
     5521 gtcgaggccg cacggcgcgg ccgcggccgt atcgtcgtcg agttcgccga cgaagccgac
     5581 ctggatcgca tcttccggtc gatcgcccgc gactgacgcg ctcacgccgc gcaccgcacc
     5641 gttcagcgag gaggacccga tggagtttcg caggatcgac cggctgccgc cgtacgtgtt
     5701 cagcgtcgtc aacgacctga aggcggccgc tcgtcgagcc ggcgaggaca tcatcgacct
     5761 cgggatgggc aaccccgacc tggccacgcc gggggtggtg gtcgaaaagc tcaccgaggc
     5821 cgcgcgcaat ccacgcaacc accgatactc gacctcgcgc ggggtgccga agcttcgcaa
     5881 agccatctgc gactggtacg agcgccaata cggcgtgacg ctcgaccccg aaaccgaagc
     5941 caccagcgtc atgggcgcca aagaaggcct gtcgcacctg gcctggacgc tggtccagcc
     6001 cggcgacacc gtgctcgttc ccgagccgac ctacccgatc cacaccttca gcttcgcgct
     6061 cgccggcgcg gacgtgcacg ctgtgccgat gagcgcgggt gccgacctgt tcgaaggcct
     6121 gctcgacgcc tacgagcgat gctggccgcg tccgcgcgtg ctgctgacga gctttccgca
     6181 caacccgacg acggcgtgcg tggacagcgc cttcttcgag cggctggtcg ccttcgccca
     6241 ggagcacagc ctgatcgtgg tgcacgactt cgcctacgcc gacctgtgct tcgacggcta
     6301 cgaggccccc tcgttcctgt cggtcccggg cgccaaggac gtcggcgtcg agatcttctc
     6361 gatgtcgaag tcgttctcga tggccggatg gcggatcgcg ttcatggtcg gcaaccccga
     6421 gatcgtcgcg gcgctgacgc ggctgaagtc gtacctcgac tacggcgtct tccagccgat
     6481 ccagatcgcc gcgatcatcg cgctcaacga gtgcggcaac gagaccaagg tcttcaacga
     6541 cgtgtatcgc acccgccggg acgcgctcat cgaaggcttg cagcgagccg gttgggacgt
     6601 gccaccgccg caggcgacga tgttcgtctg ggcggagatt cccgatgcgc acaaggcgat
     6661 gggctcgctg gagttcgcga agttcctgct cgccgagggc aaggtcgccg tctcgcccgg
     6721 cgtggggttc ggccgcgcgg gcgacggcca cgtccggttc gcgctcgtcg agaacgagca
     6781 caggattcgt caggccacac gcgggatacg caaagccctc ggaggatgat gatggccgac
     6841 gtcgtggtgg acatcaacga aggaaaagag tccgagtggc agcgcgggct cgccgggttc
     6901 ctgctcgggc tcgcgctcgg agcgctggtc gcgctgctgt cgaagcgcaa gccgggatga
     6961 cccgccgcgt cgaggacatc gacgtccgca ccgtgcgcga ccttccacac cgctgtcgcg
     7021 gctgcgtctt ctgggagctg tcgcccgccg accgagcctt cgccgtcgag cacgacgccg
     7081 agttcgagaa agaggcctgg tgcagcgagc tatcgctgac gtggggcgcg ccgggcaaga
     7141 tcgtctacgt cgatgacgag cctgccgggt tcgcgctcgc gggccccccg accgcctttc
     7201 cgcgcgcggg cttctttccg gccaaggtct ccgacgacgc gttgctgctc gcgaccatgc
     7261 acgtgctcca gagccaccag ggccgtggga tcggcaaggt gctgatccag aacgtgctca
     7321 agatggccaa ggagcacggc ctgcgggccg tcgaatgctt cgccgacaag cagtgggccg
     7381 gttacgactg cctcattccg gccgagttct gccatgccat cggattcagg atcaagcgcg
     7441 accacgttcg tttcccgttg atgcgcatcg acatcaaggc gctcgccaaa ctcgcggagt
     7501 ccttcgaggc caagctcgag tcgttcctgg aatcgctcaa ggttccgtcg accgcgccgc
     7561 ggccggttcg atagcggggt tcgcccagcg gggttcgccc tccggggttc gccctccggg
     7621 cggcgggatg cgcgcgtgcc agttccgggc ggcctgatgc gtgcggtggc ttcgtgcggt
     7681 gggacgcgtc aggcggggat cggggagacg gcgaaggtgc ggatcgcggc gaagacggcc
     7741 tcggcgatgc gccgctggaa ctccggctcg gtcaggcgcg cggcctcgtc ggggttggtg
     7801 atgtagcagg gctcgacctg gaccgcgggc atgcgcgtct cgcgcagcac gccccaggtc
     7861 ttggcgtgag agcgcccgtc ggccagcccg agcccggcga gatggtccat gatcgtctcg
     7921 gccaagcgtc ggccgccgtc ggactcccac acgtcggtgc cgaaatagaa cgccgcggcg
     7981 ccggacgcgt gtggatccgc cgaaccgccc atgtggattg ccacgtacag gtccgcgccg
     8041 agcgtgttcg cgagcgcgcc tcgctcggcc tcgccgggcg tggaggcatc gtcgcgcgtg
     8101 aggaagaccg cgacgccgct cgccgcgagc gcagcttcga gccggcgcgc gatcgcgaag
     8161 gcgacgtcgc actcgtgcag cccgcccggg ccgctgtggc cgggatcggc ggggccgtgt
     8221 ccggggtcga ccacgatcgt cagcccggcg agcgtcggcg gcagccgccg caggtgctcg
     8281 cgttcgcgca ggcgcgtgac gggcgtatcg ccgcccaggc tcggcatgcc gatgagcgcg
     8341 cggatcgtgc tcgcgccgat gatgccgtcg ggcggcaagc cgtagttgcg ctggaactcg
     8401 acgacgacgc ggatcgtctg cggaccgagg atgccgtcga tgcgtccggg atcgaagccg
     8461 agcttggaga gtcgctgctg cagttcgcgc acgtcgtcgc cgcgcatctg cggcgagcgc
     8521 acgtacagga cgcgatcgcc gaggttccag gacgcctcga ccagctcgcg ccaggtctgg
     8581 gggccgacga tgccgtcgga gatcagcccg cgccgctgct gaaacgcgcg gaccgcggcg
     8641 gttgtggacg ggccgaaccg gcggcgggcg cgctcggact cgtcgatgga cgcgccgaag
     8701 gcaagaagcc gccgctggat atcgcgaacg gcctcgcctg ccgagccttc cgtgtagagc
     8761 acgccggaat tatgcccgga ccgaggcgaa gccgttcggc cgaagtcgtt tgctatgaga
     8821 cgaactcgga gagctcgccc aggatctgct gcttgccgcg cgcgccgacg acgcggcgct
     8881 tctcgacgcc gtccacgaac agcagcatcg acgggatcga caggatgccg tagcggcgcg
     8941 agacgtcggg gttgttgtcc acgtcgagct tggcgacggt tagtttgccc tgcatctcgc
     9001 cggcgatctc ttccaggacg ggcgcgacca tgtggcacgg cccgcaccac tccgcccaga
     9061 agtccacgag gacgggcgtc gaggacttca ggaccttgtc ctcgaagtcc gagtcggtca
     9121 cggtgattgt gggtccggcc acgatcaagt cactccttcg aattcgtccg ttttgctccg
     9181 cgcgggattg taccgtcgaa gggcccaacg ttcccgggca tcttccgtat tccgttgccg
     9241 gttgcgcccg aatcgaaaca gacggctaga gggctaggcc gcaggcctgg atcttctcga
     9301 tgtcgagctc gacgcccaga cccggggctt gcggaagcga gagccggccg tcctcgtcgg
     9361 tgacgagcgg ctcggtgagc atcgcgtcgc ggccttcggg cgtccagccc ggcgggtcgt
     9421 agggatactc gagccaggcg ccgtgtgtgg ccgcgaacac gtgcagattg gccgcgaaac
     9481 cgaggccgtt ggaccaggtg tgcgggttga agccgatgcc ggcgttcttg gccatggtcg
     9541 cgatctcgag cgcgccggtg atcccgccgg tgaacatcgc gtcgggctgg atgatgtcga
     9601 gcgagcggcg ctcgatgtgc tcgcggagcg gccagatgtc gccggcgagc tcgccgccgg
     9661 cgatcggggt cgtcgtttgg tcgcgcaggc gccggtagcc ttcgaagtcg aactggtcca
     9721 gcggctcttc cagccaggcg acgtcgaact cctcgtaggc cttcgcggtg gcgagggcgc
     9781 gcttgaagtc ccagcggggc gagtcgcctt tgaacacatc gacgcgccag ccctggttgg
     9841 cgtcgcaagc gatggttagg tcgtcgccgg cggcctcgcg caccgcgcgt acctcggcca
     9901 cgtcctcccg catcgtgtcg tgccgaggcc ggagcttgac ggccttgaag ccgcgctcca
     9961 tcgcggcgag cgcgtcgtcg gcgcgttgct tcggatcgcg tacctcgccg aacgaggcgt
    10021 agacgggcac gcggtcgcgc gtgccgccga ggtagcggta cagcggcacg cccgcggctt
    10081 tggccgccag gtcgtagagc gccgcctcga cgaaccacac ccgcagaccc aggacacgca
    10141 cggcggtctc gaggatcgga cggaactcgg cgatgcgcgt cgggtcgcgc ccgataagga
    10201 acgcgcggag caggttgatc ggcccgcggc cttcgtcggc gaaggcgatc cagcccgtgt
    10261 agccctcgac gccgtcgtcg gtgacgagcc ggtagatcac gcacgagttg ttcggattgg
    10321 cgagccccgg cacccacgac ggcgagaacg gcgccgccag cggaaagtgg cagatccagg
    10381 catcgatgcg ttcgatcttc attccggtcc cccctcgcgg tcccatgcgt gctcctagcc
    10441 actcgccaaa tctgcaaccg gattgcagat ttgcgcgatc gctgcgagcg attgggtcag
    10501 tctgtcacga atgcgacgcc tcgggccagg cggcacc
//
LOCUS       DATLFO010000341         7523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_117711, whole genome shotgun sequence.
ACCESSION   DATLFO010000341 DATLFO010000000
VERSION     DATLFO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117711"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1456)
                     /locus_tag="VM841_13745"
     CDS             complement(110..1456)
                     /locus_tag="VM841_13745"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVL91291.1"
                     /translation="MPLRLRLLVAATVAGGGAIVTWHLRLAGDVSWGGVAIMAAAFLS
                     GLLIVELSNVSAESVTFALVAAALIGDGPTAAVLAAAAGEVGSALRPPVPKLHRSAYN
                     VSAHIVAAAALGWIDAWAGAAAAVPVRVLIATAGGLVYFGISSGLVAAAIGWSTKAQP
                     LAVWSQAFRWLAPHYAAFGLAGFVLATVVRVSGPAGVLVFLLPLLMSHYSVWIFVRRT
                     REQMQQLESANRRLASYNAELTEALASVVDARDLYLFRHSHQASAYSERIARRLALPE
                     DQVRLVRQGALLHDIGKVGIPEAILRKPGPLSEAERSIMNRHAAIGASIVARTSELTP
                     VAAIIRAHHERFDGRGYPDGLAGDEIPVGARILSVLEAFDAMISDRPYRAAMSVEDAL
                     AEIGRCSGSQFDPRVVRAFLSLVEEEGSDWLHNSAAGAHVTGTDLFLPLAERTGTA"
     gene            complement(1465..2835)
                     /locus_tag="VM841_13750"
     CDS             complement(1465..2835)
                     /locus_tag="VM841_13750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HVL91292.1"
                     /translation="MSASVRSKRLTAVVAVLAAALSMLAVPARDGSSVPVMVLEARPA
                     SQAAESVVRAAGGRIGASLDLIGGFAARIPAAAIRSLLARPEIAGVLPDAQLRLAGKG
                     GGGGGTPATSSTFAAAVDAPRAWSGGITGRGVNVAVLDSGIAPVADLTAPANRIVAWK
                     DFVSGSPSPVDPHGHGTFVAGIIAANGAASGGARKGIAPEAGLVGVRVFDEFGSATAS
                     RVIQGIQWIIQNRATHGIRVVNMSFSTTSDLSYKIDAVTYAAEQAWRAGIVVVASAGN
                     AGPAPGTILSPGIDPFVITVGASDDRGTAARDDDTIASFSGAGPTRLDLIAKPDLIAP
                     GVWTTGLRVPGSLVDVQHTLSRRDEHYFDGSGTSPAAGVVSGVAALMLHKRPKLDPDQ
                     VKAVLKQTTTPAPFSLLPGQAGAGVVNASNAASSRITSRDNAGHVPASGGGLLSLVGI
                     PDVKWR"
     gene            complement(3014..3841)
                     /locus_tag="VM841_13755"
     CDS             complement(3014..3841)
                     /locus_tag="VM841_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL91293.1"
                     /translation="MRAKIWGCRGSLAAPGPDTVRYGGNTSCVEVELDDGSLIILDAG
                     TGIRTLGLREMPRKVHLLLTHLHLDHLEGLGFFSALWRSECEITVYGPSSPMKSLIRR
                     IEGYLSPPLFPVHLSDVPSKLTFLDAPDGEWSIGPARMFSQHITHSGPAVGYRIEENG
                     VSLAYMPDHEPFRGGVRDVDPAWLSGHAIAHGADVLLHDAQYTEAEYPSRIGWGHSAV
                     DQVVAFGSAAKVGKLLLFHHDPLHTDADLDAQLARAKELTDGQFDVDAAREGETIDL"
     gene            complement(3860..4261)
                     /locus_tag="VM841_13760"
     CDS             complement(3860..4261)
                     /locus_tag="VM841_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL91294.1"
                     /translation="MERTQYKSLALILARDLAENLATPIFVVDATGALVFYNEPAEHI
                     LGQRFAETGELPAAEWASIWVPTEVDGSPMQIQDMPIGIALQNQRPAHRSMRIKGLDG
                     VSRLIQVSAFPLLASPTEFSGAVAIFWEEPE"
     gene            4400..5614
                     /locus_tag="VM841_13765"
     CDS             4400..5614
                     /locus_tag="VM841_13765"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HVL91295.1"
                     /translation="MTTIAWFRRDLRVHDNPALSSAMSAGDPVACLFVVEPGWLKVCP
                     ARSAAFAHAVAGLAAELERRGAKLIVRAGPAAEAVPALARETGASRVLAAGDVTPAAV
                     RRDKAVARALDETGISFRSTPGLLVRDPGAVRSSSGDPYKVFTPYHKAWLAVPPRDRW
                     SAPSEIPGVSADSHEGALAALAAARTAHEPALAVAEREARRALRAFLGESLGGYDECR
                     HRLDGTGGSMLSAALRTGALSSLECATEAAPHRGSERWIRQLAWRDFASQTAARWPAL
                     FRQDLRKRKGQWSRDAHAAEEWRTGTTGVATVDAAMRQLASQGWISNRARMMVGSFLV
                     RDMNIHWREGARHFMRTLADGDPAVNAFNWQWVAGTGYDVPAPYWRLSPDRQQERHDP
                     GAAWLMRWLGRA"
     gene            complement(5611..6579)
                     /locus_tag="VM841_13770"
     CDS             complement(5611..6579)
                     /locus_tag="VM841_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVL91296.1"
                     /translation="MPAVFRDPLLLARLEPVAPGATERAVNALTTAPSGYEGEGFPVR
                     RAFAGVPLAELDPFIHMDQMGEVEYAPGEPRGTSWHPHRGFETVTYMIDGTFQHQDSH
                     GGGGLITDGATQWMTAGRGILHIETPPEQLVISGGLFHGIQLWVNLPRDKKLSPPRYQ
                     GLESEQVALVASHDGASLLRVIAGEVGGVRGPGDTHTPIGFVHASVSPGARLRVPWRA
                     DFNALVYVLAGYGTVGAEAHRIGGGQLAVFGPGDTLTIAADEDQESRSPALEVLILGG
                     RPIREPVAAYGPFVMNTRDELIQAFEDFQAGRLGVVPPGALQPYHP"
     gene            complement(6612..>7523)
                     /locus_tag="VM841_13775"
     CDS             complement(6612..>7523)
                     /locus_tag="VM841_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVL91297.1"
                     /translation="AQGAAVVAKPLWFLAVYVIVVAAAPPMLALHRRFGLRVPVALAV
                     AAVAVDVARIGFDVPLVGWLNFAFVWLLPHQLGFFYADGSLVAAGRRIAAMLAAGGVA
                     AVALLVGSGIYSPSMVGVADGRVSNNSPPGVALAALSLWLVGLVMLARPHLVSWLGRR
                     RVWTAVIGGGSMAMTVFLWHLTALMILAPVALPLGFPQPEAGTASWWLTRPLWIAMLA
                     LVTAPLVLVFARFERPKRRAASPEPAAPRVLAAAALLVPALAGFATYGFTGVTDAGWL
                     RPPMSAAAFAGALWLLRRREQPGAGVC"
BASE COUNT         1191 a         2660 c         2661 g         1011 t
ORIGIN      
        1 ccgtggatga tcgtcatcga cgttcgcaga tcgtgcgcga gcagcgcgag cgcatcccgg
       61 ctcgcgatcc ccgatcgcgc ggcccgcgcc agcggctgat cgtcgatcat cacgccgtcc
      121 cggtccgttc ggccagcggc aggaacagat ccgtgccggt gacgtgcgcc ccggcggccg
      181 agttgtgcag ccaatccgat ccttcctcct ccaccagcga caggaacgcc cgaacgaccc
      241 gtgggtcgaa ctggcttccg ctgcagcggc cgatctcggc gagtgcgtcc tcgaccgaca
      301 tcgccgcgcg atacggccgg tcgctgatca tggcatcgaa cgcctcgagg acgctgagaa
      361 tgcgcgcgcc gacggggatc tcgtctcccg cgagcccgtc gggatagccc cgtccgtcga
      421 agcgttcgtg gtgcgcgcgg ataatcgccg cgacgggcgt cagctcgctg gtccgcgcca
      481 cgatcgacgc cccgatcgcc gcgtgccggt tcatgatgga ccgctcggcc tcggacagcg
      541 gtccgggctt gcgaaggatc gcttccggaa tgccgacctt gccgatgtcg tgcagcagcg
      601 cgccttggcg gacgaggcgc acctggtcct cgggcaaggc gagccggcgg gcgatgcgtt
      661 cgctataggc gcttgcctga tgcgaatgcc gaaacaggta caggtctcgc gcgtcgacga
      721 ccgacgcgag cgcctcggtc agctccgcgt tgtaggacgc gagccggcga ttcgccgact
      781 cgagctgctg catctgctcg cgggtccggc gaacgaagat ccataccgag taatgggaca
      841 tcagcagcgg cagcaagaac acgagcaccc ccgccggtcc gctcacgcga acgacggtgg
      901 cgaggacgaa tccggcgagc ccgaacgcgg cgtagtgcgg cgcgagccac cggaacgcct
      961 gcgaccacac cgcgaggggc tgcgcctttg tgctccatcc gatcgcggcg gcgaccaagc
     1021 ccgaggagat cccgaagtag accaacccgc cggcggtcgc gatcagcacc cgcacaggaa
     1081 cggcggcggc ggcgcccgcc cacgcgtcga tccagccgag cgccgcggcc gcgacgatgt
     1141 gcgccgagac gttgtaggca gaacggtgca gcttgggaac cggcggccgg agcgcgcttc
     1201 ccacctcgcc ggcggccgcg gccagcacgg ccgctgtcgg cccgtcgccg atcagcgccg
     1261 ctgcgaccag cgcgaacgtc acggactcgg ccgacacgtt cgacagctcc acgatcagca
     1321 ggccggacag gaacgcggcg gccatgatgg ccacaccacc ccaggaaaca tccccggcga
     1381 gccggaggtg ccatgtcacg atggcacccc cgcccgccac cgtggcggcc acgagcagac
     1441 gaagtcggag gggcatcgtc cgccttaccg ccacttcacg tcgggtatcc cgacgaggga
     1501 gaggagaccg ccgccggatg cgggcacatg cccggcgttg tccctcgagg tgatcctgct
     1561 ggaggctgcg ttggaggcgt tgacgacgcc cgcacctgcc tgtccgggca agagcgagaa
     1621 gggcgcgggc gtcgtcgttt gcttgagaac ggccttgacc tgatccgggt cgagcttcgg
     1681 gcgcttgtgc agcatcagcg cggccacgcc gctgaccacg cccgcggccg gtgaagtgcc
     1741 gctgccgtcg aagtagtgct cgtcgcggcg cgacagcgtg tgctgaacat cgacgaggct
     1801 tccgggaacg cgaaggccgg tcgtccacac gccgggcgcg atcagatcgg gcttggcgat
     1861 caggtcgagc cgcgtcgggc cggcgccact gaaggacgcg atcgtgtcgt catcgcgcgc
     1921 ggcggtgccg cgatcgtcgg aggctccgac ggtaatcacg aacggatcga tgcccgggga
     1981 aaggatcgtt ccgggagccg ggcccgcgtt gccggcggag gcgacgacca cgatgccggc
     2041 gcgccacgcc tgctcggcgg cgtaggtgac ggcgtcgatc ttgtacgaca agtccgaggt
     2101 cgtcgagaac gacatgttga ccacgcgaat gccgtgcgtg gctcggttct ggatgatcca
     2161 ctggatccct tggatgacgc gcgaagcggt tgcgctgccg aattcgtcga agacgcgcac
     2221 gccgaccagg ccggcctcgg gggcgatgcc tttgcgcgcg ccgccggagg ccgcgccgtt
     2281 ggcggcgatg atgcccgcca cgaacgtccc gtgcccgtgc gggtccaccg gcgacggcga
     2341 gcccgagacg aaatccttcc acgcaacgat gcggttggcc ggcgcggtga ggtctgcgac
     2401 gggtgcgatc ccggagtcga ggacggcgac gttgacgccg cgtccggtga tcccgccgct
     2461 ccacgcccgg ggagcgtcaa ccgcggccgc gaacgtcgag gacgtcgcgg gcgtcccccc
     2521 tccgccgccc cccttgcccg cgagccgaag ctgggcgtcg ggaagaactc cggcgatctc
     2581 gggccgggcg agcagcgatc ggatcgcagc cgccgggata cgcgccgcga agccgccgat
     2641 cagatcgagc gatgcgccga tgcgcccccc ggccgcgcgc acgacgctct cggcggcctg
     2701 ggaagccggt cgcgcctcga gaaccatgac cggaaccgac gatccatcgc gtgcgggcac
     2761 ggcaagcatc gaaagggccg cggccaacac cgcgacgacg gccgtgaggc gcttgctgcg
     2821 aacgctcgcg ctcattcgga tctcgctctc ctctgcgttc ctcgggccgg gccgtccccg
     2881 gcgtgcggag atgggacacg ggaagtgtcg gggagcggga cgtcccggga aatggctcgc
     2941 gctaccgaga tcgcgtgagg gcagggccgc cgagcattag ggacggcgcg ggaccgatga
     3001 tcccgccggg accctacaga tcgatggtct cgccttcgcg ggcagcgtcg acgtcgaact
     3061 gcccgtcggt cagctctttg gcgcgagcga gctgcgcgtc caggtcggca tcggtgtgga
     3121 gcgggtcgtg gtggaacagc aacagcttgc ccacctttgc ggcgctgccg aacgcgacga
     3181 cctgatcgac cgccgaatgc ccccacccga tccggctggg atactccgcc tcggtgtatt
     3241 gcgcgtcgtg gagcagcacg tcggcgccgt gggcgatcgc atggcccgac agccacgcgg
     3301 gatcgacgtc gcgcaccccg ccccggaacg gctcgtgatc gggcatgtac gcgagcgaaa
     3361 cgccgttctc ctcgatgcgg tagcccacgg ccggccccga gtgggtgatg tgctggctga
     3421 acatgcgcgc cggcccgatc gaccattcgc cgtcgggcgc gtcaaggaac gtgagcttcg
     3481 acgggacgtc ggacagatgc acgggaaaca gcggcggcga caggtatccc tcgatgcggc
     3541 ggatgagcga cttcatcggc gaggacggcc cgtagacggt gatctcgcac tcgctgcgcc
     3601 agagcgcgct gaaaaagccg agcccttcca gatgatccag atgcaagtgc gtcagcagca
     3661 ggtggacctt cctgggcatc tcgcgaagac ccagcgtccg gatgccggtg cccgcatcca
     3721 ggatgatcag cgaaccgtcg tcgagctcga cctcgacgca cgaggtgttg ccgccgtacc
     3781 gcacggtatc gggccccggc gcggcgagcg agccacggca tccccatatc tttgcgcgca
     3841 tcgaatcccc atccgagccc tactcgggct cctcccaaaa gatcgccacg gcaccggaga
     3901 actcggtcgg gctggcgagc agcgggaacg cggaaacctg aatcaggcgc gagacgccgt
     3961 ccagtccctt gatcctcatg ctgcggtgcg ccggccgctg gttctgaagc gcgatgccga
     4021 tcggcatatc ttgaatctgc atggggctgc cgtccacctc ggtcggcacc cagatgctgg
     4081 cccattcggc ggcggggagc tccccggtct cggcgaaccg ctgccccaga atatgctcgg
     4141 cgggctcgtt gtagaagacc agagcaccgg tggcgtccac gacgaagatc ggggtcgcga
     4201 gattctcggc gagatcccga gcgaggatca acgcgaggct tttgtactga gttcgctcca
     4261 tccgctccgc agcgtaccgg cctgcgagcc gcccggacaa gagctcggtt gaccgaggtc
     4321 acgcagaaag cgtgtcgaag cgcacacttc ctgcggcgtt gccgagcccg gccggcccgc
     4381 cgacaatacc cgcataccca tgacgaccat cgcctggttc cgccgcgacc tgcgagttca
     4441 cgacaatccg gcactttctt cggccatgtc ggccggcgat ccggtggcat gcctgttcgt
     4501 ggtcgaaccc ggctggctga aagtctgccc cgctcgcagc gcggcgttcg cgcacgcggt
     4561 cgccgggctc gccgccgagc tcgagcgacg cggcgcgaag ctgatcgttc gggccggacc
     4621 tgctgccgag gcggtccccg cgctggcgcg cgagaccggc gcgtcgcgcg tgctcgcggc
     4681 gggcgacgtc acgccggcgg ccgtccggcg ggacaaggcg gtcgcgcgag cactcgacga
     4741 gaccggcatc tcgttccgct cgacgccggg gctgctcgtg cgggatcccg gcgcggtgcg
     4801 ctcctcttcg ggcgatccgt acaaggtctt tacgccctac cacaaggcgt ggctggccgt
     4861 gccgccgcga gaccgatgga gcgcgccgtc ggagatcccg ggcgtgtccg ccgattcgca
     4921 cgagggcgcg ctcgcggcgc tcgccgcggc gcgcaccgcg cacgaacccg cgctcgccgt
     4981 cgccgagcgt gaggcgcgac gcgccttgcg cgcgttcctg ggcgagtccc tcggcggcta
     5041 cgacgaatgc cgccaccggc tcgacggcac cggcggaagc atgctgtcgg cggcgctgcg
     5101 caccggcgcc ctgtcatcgc tggaatgcgc aacggaagcg gcgccgcatc gcggctcgga
     5161 gcgatggatc cgccagctcg cgtggcggga cttcgcgtcg cagaccgcag cgcgctggcc
     5221 tgcgttgttc cggcaggatc tgcgcaaacg caaagggcaa tggtcccgcg acgcgcacgc
     5281 ggccgaggag tggcgcaccg gcacgaccgg cgtggcgacc gtggacgcgg ccatgcgcca
     5341 gctcgcgtcg cagggatgga tctcgaaccg cgcgcgcatg atggtgggca gcttcctggt
     5401 ccgggacatg aacatccact ggcgcgaagg ggcgcggcat ttcatgcgga cgctcgccga
     5461 cggcgatccc gccgtcaacg cgttcaactg gcagtgggtg gcggggaccg gctacgacgt
     5521 cccggctccg tattggcggc tcagtcccga ccgtcaacag gagcggcacg atccgggtgc
     5581 cgcgtggctg atgcgctggc tcggccgcgc ctaagggtga tagggctgga gcgcgcccgg
     5641 cggcacgacg ccgagccggc cggcctggaa atcttcgaac gcctggatca gctcgtcgcg
     5701 cgtgttcatc acgaacggtc cgtaggcggc gacgggctca cgaatcggcc gtccgcccag
     5761 gatcagcacc tcgagcgcgg ggctgcgcga ttcctggtcc tcgtcggccg cgatcgtgag
     5821 cgtgtccccc ggtccgaaga cggcgagctg cccgcccccg atgcggtgcg cctcggctcc
     5881 cacggtgccg tagcccgcga gcacgtagac gagcgcgttg aagtccgccc gccacggcac
     5941 gcgcagccgc gcacccggcg acaccgaggc gtgcacgaac ccgatcggcg tgtgggtgtc
     6001 ccccggcccc cgcacccctc cgacctcacc ggcgatcacc cgcaggagcg atgcgccgtc
     6061 gtgcgatgcg acgagcgcca cctgctcgct ttcgaggccc tggtagcgcg gcggggacag
     6121 cttcttgtcg cgcggcaggt tcacccaaag ctgaatgccg tggaacagcc cgccgctgat
     6181 gacgagctgc tcgggcggcg tctcgatatg caggatgccg cgcccggccg tcatccactg
     6241 ggtcgcgccg tcggtgatca gcccgccgcc gccgtgcgag tcctgatgct ggaaggtgcc
     6301 gtcgatcatg tacgtgaccg tctcgaatcc gcggtgcgga tgccacgacg tgccgcgcgg
     6361 ctcgcccggc gcgtactcga cctcgcccat ctgatccatg tggatgaacg ggtcgagctc
     6421 ggcgagcggc acgcccgcga acgcgcgccg caccggaaaa ccttcgcctt cgtagccgct
     6481 cggcgcggtc gtgagcgcgt tgacggcgcg ttcggtcgcg ccgggagcga ccggctccaa
     6541 gcgagcaaga agcagcggat cacggaacac ggcgggcatc tcggacctcc gtcgtcgggc
     6601 tctcgccgct atcaacatac tcccgcgccc ggttgttccc gccggcgaag cagccacagg
     6661 gcgcccgcga acgccgcggc gctcatcggg ggccggagcc agccggcgtc ggtgacgccc
     6721 gtgaacccgt aggtggcgaa gccggcgagc gccggcacca gcagcgcggc ggcggcgagc
     6781 acccgcgggg ccgccggctc gggactcgcg gcccggcgct tcggccgctc gaagcgggcg
     6841 aagacgagca cgagcggcgc cgtaaccaga gccagcatcg cgatccacag cggcctggtc
     6901 agccaccacg aggccgttcc cgcctcgggc tgcgggaagc ccagcggcaa ggcgaccgga
     6961 gccaggatca tcagcgcggt cagatgccac aaaaagacgg tcatcgccat gcttccgccg
     7021 ccgatgacgg cggtccagac ccggcggcgg ccgagccagg agaccaggtg cggacgggcc
     7081 agcatcacca ggccgacgag ccacagcgat agcgcggcca gcgcgacgcc gggcggcgag
     7141 ttgttggata cccgtccgtc ggcgacgccg accatgctcg gcgagtagat gcccgacccc
     7201 accagaagcg ccacggccgc gacccctccc gccgccagca ttgcggcgat gcgacggccc
     7261 gccgccacca gcgagccgtc ggcgtagaag aatccgagct ggtgcggcag cagccacacg
     7321 aaggcgaagt tgagccagcc cacgagcggc acgtcgaagc cgatccgcgc cacgtccacc
     7381 gcgaccgccg cgacggcgag cgcgaccggg acgcgaaggc cgaagcggcg gtggagcgcg
     7441 agcatcggcg gcgcggctgc cacgacgatg acgtagacgg ccaggaacca cagcggcttg
     7501 gcaacgacgg cggctccttg cgc
//
LOCUS       DATLFO010000342         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_126661, whole genome shotgun sequence.
ACCESSION   DATLFO010000342 DATLFO010000000
VERSION     DATLFO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126661"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VM841_13780"
     CDS             complement(<1..156)
                     /locus_tag="VM841_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVL91298.1"
                     /translation="MPAAEARSITVGRFIADALAAAGVRWAFTVPGESFLPLLEALPQ
                     AGVRVVAT"
     gene            complement(272..769)
                     /locus_tag="VM841_13785"
     CDS             complement(272..769)
                     /locus_tag="VM841_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HVL91299.1"
                     /translation="MAGDVSFDVVSDFDRQELVNALDQARREIATRYDFKGARAELEL
                     GKDEITLRADSETRARSMRDLLESKAIRRGLSLKIFDWGAIEDAGGNTIRQRIGLRRG
                     LPEDLARKISKLIRDEFPKSKSQIQGDAVRVSGKSKDELQRVIVRLRQEGEGYPVALQ
                     FTNYR"
     gene            complement(759..1190)
                     /locus_tag="VM841_13790"
     CDS             complement(759..1190)
                     /locus_tag="VM841_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL91300.1"
                     /translation="MPDPLTHGDAQDLLAAFKRGWEKRDPDLIMALFGAEAEYREHPF
                     AESLVGANAIRALWNDICASQDHVEFDAERIWLSGTTVLASWHAAYTQQDTAERRRVR
                     GFMTLELGDDRLVRRYRGWPTERAVGTDSTFQREVVTADGR"
     gene            1259..>2182
                     /locus_tag="VM841_13795"
     CDS             1259..>2182
                     /locus_tag="VM841_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL91301.1"
                     /translation="MRITFLGQAGLYIETARASILCDPWFNPAYFGSWFPFPANDGID
                     PRSIGNPTFLYVSHLHHDHFDPRWLTEHCSKEATVILPDYPLETLEKELRGLGFSRFI
                     KTVNEEPFEAEGLRMLVMALVAPTDGPIGDSALLVDDGDVRVLNMNDSRPIDPERLLV
                     FGPLDALFLQFSGAIWYPMVYEFPERARQVLGHKKRVAQSARAYRYIELLEPAFVVPS
                     AGPPCFLDDDLFEHNDLAGDESNIFPDQPTFLSYVAERGHAGGRLMLPGSVADVARGR
                     FDVTHPVPPGSIFDDKRAYLSAYRERKRDVLA"
BASE COUNT          317 a          782 c          722 g          361 t
ORIGIN      
        1 cgtcgcgacg acgcggacgc cggcctgcgg cagcgcctcg agcagcggca ggaagctctc
       61 gcccgggacg gtgaaggccc agcgcacgcc ggccgcggcg agagcgtcgg cgatgaaccg
      121 gccgacggtg atcgagcgcg cttcggcggc gggcactccg gccgtcccta gagtgcctgg
      181 tagacggcgc cgaagaccag gctgagcacg accatcgcga ccagaatgat gatcacggcc
      241 cgcacccagc ggtcgttggt gtacacgccg ctcagcggta gttcgtgaac tggagcgcga
      301 ccgggtagcc ctcgccttcc tggcgcaggc gcacgatgac gcgctgcagc tcgtccttgc
      361 tcttgccgct gacgcgcacc gcgtcgccct ggatctgcga cttcgatttg gggaactcgt
      421 cgcggatcag cttgctgatc ttcctggcca ggtcttccgg caggccgcgg cgcaggccga
      481 tccgctgcct gatggtgttg ccgccggcat cctcgatcgc gccccaatcg aagatcttga
      541 gcgacaggcc gcgccggatc gccttcgact cgagcaggtc gcgcatggac cgggcgcggg
      601 tctccgagtc ggcgcgcagc gtgatctcgt ccttgcccag ctcgagctcc gcccgcgcgc
      661 ccttgaagtc gtagcgcgtg gcgatctcgc gccgcgcctg gtcgagcgcg ttgaccagct
      721 cctgccgatc gaagtcactg accacgtcga acgacacgtc acctgccatc tgccgtcacc
      781 acctcacgct ggaacgttga atctgtgccc acggcccgct ccgtcggcca gccgcggtag
      841 cgccggacga gccggtcatc gcccagctcg agcgtcatga agccccggac gcggcgccgc
      901 tcggcggtgt cctgctgcgt gtaggcggcg tgccacgatg cgagcacggt ggtgccgctg
      961 agccagatcc gctcggcgtc gaactcgacg tgatcctggc tggcgcagat gtcgttccac
     1021 agcgcgcgga tggcgttggc cccgacgagc gactcggcga aggggtgctc acggtactcc
     1081 gcctcggcac cgaagagggc catgatcagg tccgggtcgc gcttctccca gccgcgcttg
     1141 aatgcggcca gcaggtcctg cgcgtcgccg tgggtgagcg gatcgggcat gtcgctcgaa
     1201 gtctacgtcg cgccgggcga ggccggccat tcggccgatg attggtaagg tgagtcgcat
     1261 gcgcataacc ttcctcggcc aggccggcct gtacatcgaa acggcgcgcg ccagcatcct
     1321 ctgcgacccc tggttcaacc cggcctactt cggctcgtgg ttccccttcc cggccaacga
     1381 cggcatcgat ccgcgctcga tcggcaaccc gaccttcctc tacgtcagcc atctccacca
     1441 cgatcacttc gacccgcgct ggctgaccga gcactgctcg aaggaggcga cggtcatcct
     1501 gcccgactac ccgctcgaga cgctcgagaa ggagctgcgc gggctgggct tcagccgctt
     1561 catcaagacg gtcaacgagg agccgttcga ggctgaaggg ctgcgcatgc tggtgatggc
     1621 gctcgtcgcg ccgaccgacg ggccgatcgg cgattcggcg ctgctcgtcg acgacggcga
     1681 cgtgcgcgtg ctgaacatga acgattcgcg ccccatcgac ccggagcggc tgctcgtctt
     1741 cgggccgctc gacgcgctct tcctgcagtt cagtggcgcg atctggtacc cgatggtcta
     1801 cgagttcccg gagcgggcca ggcaggtgct cgggcacaag aagcgcgtcg cccagtcggc
     1861 gcgcgcgtac cgctacatcg agctgctcga gccggccttc gtcgtgccct ccgccgggcc
     1921 gccgtgcttc ctcgacgacg atctgttcga gcacaacgat ctcgccggcg acgagtcgaa
     1981 catctttccc gaccagccga ccttcttgtc ctacgtcgcc gagcgcggcc acgccggcgg
     2041 ccggctgatg ctgcccggct ctgtcgccga cgtcgcccgc ggccggttcg acgtgacgca
     2101 tccggtgccg cccgggtcga tcttcgacga caagcgcgcc tatctgtcgg cctaccgcga
     2161 gcgcaagcgc gacgtgcttg cc
//
LOCUS       DATLFO010000343         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_78409, whole genome shotgun sequence.
ACCESSION   DATLFO010000343 DATLFO010000000
VERSION     DATLFO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78409"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VM841_13800"
     CDS             <1..270
                     /locus_tag="VM841_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HVL91302.1"
                     /translation="PELIPLCHPVRTTFVGVEIGAGPDKASIEIRATVRGRDRTGFEI
                     EALVAASTAAITLYDFAKAEDAAMRIDGLRIVSKSGGKSGSVTYE"
     gene            267..728
                     /locus_tag="VM841_13805"
     CDS             267..728
                     /locus_tag="VM841_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HVL91303.1"
                     /translation="MKVAVLTVSDRRSRGEEPDEGGPLCADLAREIGGEVVETAIVPD
                     ERDAIAKALAAWCDAGAADLILTTGGTGLGPRDVTPEATRAVIEREAPGLAEAMRAAG
                     MKLTPYAALSRHVCGTRGSTLIVNLPGAPKAIRECFDGLREILPHAVEMMR"
     gene            725..1165
                     /locus_tag="VM841_13810"
     CDS             725..1165
                     /locus_tag="VM841_13810"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HVL91304.1"
                     /translation="MNPIVVTAVTGEPIDVAAFVARIRRPECGGLVAFEGSTRSPSQG
                     RRVQQLEYEAWLERAEAVLDELAKQAVRTYGLGGAVAVHRTGVVPIGEPSVLVAASSP
                     HRAQAFEAARWLIDTIKEQAPVWKKEIFEDGAAWVNLDIGRDEA"
     gene            1162..1533
                     /locus_tag="VM841_13815"
     CDS             1162..1533
                     /locus_tag="VM841_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91305.1"
                     /translation="MKRGKLYLIDWDEASAAEAAQALEADGWSVRIESIDGARAVKHM
                     MDDPADVVAIDLSRLPSHGRETARAVRGAKVTAATPIVFYGGADDARSKAAGAVPDAT
                     LTTAEDLPHVLARLAMRPAEG"
     gene            complement(1530..>2414)
                     /locus_tag="VM841_13820"
     CDS             complement(1530..>2414)
                     /locus_tag="VM841_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91306.1"
                     /translation="GRTGPAPPHRSGRSLLTVTGGLDVGHGEMLSSMAAALRSCGAAA
                     DVLDRREIAERFPGFALDASTGVWSPDTGVLAAADAVRAMLEAGGVSVRRPVTVTRID
                     PGPDSVTLETSQGPLRARRVIVTAGGWNGPLLETAGMTLPLQVTREQIFYFRGGEGLP
                     VLIERAALFRYMVPPLYGAPGAKVGEHTAGERTTADGRSFEIDPEGEARVADWVREQM
                     PALEAEPVAAETCLYTMTPDEDFIIDLRGPLAFASPCSGHGFKFAPLIGEILAALATD
                     TDPPVDLERFRAGRFGAF"
BASE COUNT          360 a          842 c          893 g          319 t
ORIGIN      
        1 cccgagctca tcccgctgtg ccatccggtg cgcaccacgt tcgtgggcgt ggagatcgga
       61 gccggcccgg acaaggcgtc gatcgagatt cgcgcgaccg tgcgcggccg ggacaggacc
      121 ggcttcgaga tcgaggcgct cgtcgcggcc tcgaccgccg cgatcacgct ctacgacttc
      181 gcgaaggccg aagacgcggc gatgcgcatc gacggactgc ggatcgtgtc caagtccggt
      241 ggcaagtccg ggagcgtgac ctacgaatga aggtcgccgt cctgaccgtc agcgatcgcc
      301 gttcccgcgg agaggagccc gacgagggcg ggccgctttg cgcggatctc gcgcgcgaga
      361 tcggcggtga ggtcgtcgag accgcgatcg ttcccgacga gcgcgacgcg atcgccaagg
      421 cgctcgccgc ctggtgcgac gccggcgcgg ccgacctgat cctgaccacc ggcggcaccg
      481 gcctgggccc gcgcgacgtt acgcccgaag ccacgcgcgc cgtcatcgag cgcgaggcgc
      541 cgggtctggc cgaggcgatg cgcgcggccg ggatgaagct cacgccgtac gcggcgctgt
      601 cgcgtcacgt gtgcggtacg cgcggctcga cgctgatcgt gaatctgccg ggcgcgccga
      661 aagcgatccg cgaatgcttc gacgggctcc gcgagatcct gccgcacgcg gtggagatga
      721 tgcgatgaat ccgatcgtgg tgaccgccgt caccggcgag ccgatcgacg tggccgcctt
      781 cgtggcgcgc atccgccggc ccgagtgcgg cggcctggtc gcgttcgaag gctcgacgcg
      841 atcgccgtcg cagggccgcc gggtccagca gctcgaatac gaggcgtggc tcgagcgcgc
      901 cgaggcggtg ctcgacgagc tcgcgaagca agcggtgcgc acctacgggc tcggcggcgc
      961 ggtcgcggtt caccggaccg gcgtggttcc gatcggcgag ccgtccgtgc tcgtggcggc
     1021 atcctcgccg caccgcgccc aagcctttga ggcggcgcgc tggctgatcg atacgatcaa
     1081 ggagcaggcg ccggtctgga agaaggagat cttcgaagac ggcgcagcat gggtgaacct
     1141 ggacatcggg agggacgagg cgtgaagcgc ggaaagctgt atctgatcga ctgggacgag
     1201 gcgtcggccg ccgaggccgc gcaggcgctc gaggccgacg gctggagcgt tcgcatcgag
     1261 tcgatcgacg gcgcccgcgc cgtcaagcac atgatggacg acccggccga cgtggtggcc
     1321 atcgacttgt cccgtctgcc gtcgcacggc cgggagaccg cgcgcgcggt ccgcggcgcg
     1381 aaggtgaccg cggcgacgcc catcgtcttc tacggcggcg ccgacgacgc ccggtccaag
     1441 gccgcgggcg ccgtgccgga cgcgaccctg accaccgcgg aagatcttcc gcacgtgctg
     1501 gcgcggctgg cgatgcgccc ggcggagggt tagaaggccc cgaaccggcc cgcgcggaac
     1561 cgttcgagat cgaccggcgg gtccgtatcg gtcgcgagcg ccgccaggat ctcgccgatc
     1621 agcggcgcga acttgaagcc gtgacccgag cagggcgagg cgaaggcgag cggcccgcgg
     1681 aggtcgatga tgaaatcctc gtccggcgtc atcgtgtaca ggcaggtctc ggccgcgacc
     1741 ggctcggcct ccagtgccgg catctgctct cgcacccagt ccgcgacccg cgcttcgcct
     1801 tccggatcga tctcgaagct gcggccgtcg gcggtggtgc gttcgcccgc ggtgtgctcg
     1861 ccgaccttgg cgccgggggc gccgtagagc ggcggcacca tgtagcgaaa cagcgccgcg
     1921 cgctcgatca gcacaggaag accttcgccg ccgcggaagt agaagatctg ctcgcgcgtg
     1981 acctgcagcg gcagcgtcat cccggcggtc tccagaagcg gaccgttcca tccgcctgcc
     2041 gtcacgatga cgcggcgggc gcgaagcggt ccttgcgagg tctcgagcgt gaccgagtcc
     2101 ggaccgggat cgatgcgggt caccgtcacc ggccgccgca cgctcacacc gccggcctcg
     2161 agcatcgccc gcaccgcgtc ggccgcggcg agcacgccgg tgtcgggcga ccacacgccc
     2221 gtggacgcgt cgagcgcgaa gcccggaaag cgctcggcga tctcgcgccg gtccagcacg
     2281 tcggcggcgg cgccacacga gcgcagcgcc gcagccatgc tcgagagcat ctcgccgtgc
     2341 ccgacgtcga gcccgccggt gaccgtcaga agcgagcggc cgctccgatg tggtggtgct
     2401 ggaccggttc gacc
//
LOCUS       DATLFO010000344        18747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_67598, whole genome shotgun sequence.
ACCESSION   DATLFO010000344 DATLFO010000000
VERSION     DATLFO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67598"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..506)
                     /locus_tag="VM841_13825"
     CDS             complement(6..506)
                     /locus_tag="VM841_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HVL91307.1"
                     /translation="MSDPIDDRGDLDAVIDMVADAAKRYLSTIDARPVVTFDAADHRE
                     RLPRDFPERGLGSMRAVRALVEDGLAAATASSGPRFFHFVIGGVTPAALGADWLTSLI
                     DQNGALWPSSPLSARLEQHSIGWLLDLFDLPLSWGGTLTPSATYANFAGLAAASTSTP
                     CCCAHQ"
     gene            complement(516..2273)
                     /locus_tag="VM841_13830"
     CDS             complement(516..2273)
                     /locus_tag="VM841_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVL91308.1"
                     /translation="MSEPIVIANCSGFFGDRLAAAREQVEGGHIDVLSGDWLAELTML
                     ILARSKMKNPEAGWARTFLTQLEDVLGTCLDRGIKIVSNAGGLNPAGCADAVRALAEQ
                     LGLSPRVAHVEGDDVLSRIDEFRAAGVELRNADTGEPLDGVHPITANAYIGGWGIADA
                     LERGADVVVTGRSTDAALVVGPAAWRFGWKRDDWDRLAGAVVAGHVIECGAQATGGNY
                     AFFEEVPGIEHVGFPIAEVSEDGSSVITKHAGTGGLVSVGTVTAQLVYEVASPRYHNP
                     DVTARFDTIELAQDGADRVRISGVKGEPPPPTTKVGVNTLGGFRNSFTFVLTGLDIDK
                     KAAVAEATLWASIPGGKDAFREVDVQLLRTDHDDPRTNEQALAYLKVTVIDSDPQKVG
                     RKAFGNAAIEMALATYPGFFGTSLPDEGSPYGVYMPVLIPNELVSQTVVIDGERHAVE
                     PVAIPETFDEPPAPPRVRNFDHDGSTVRAPLGRVAGARSGDKGGNANLGVWTRDATAF
                     EWLSGFLTVGKFKELITEARDLNVERFDMPNICALNFVVHGLLGKGVAASNRIDPQAK
                     GLGEYLRARIVDLPESLLR"
     gene            complement(2318..3274)
                     /locus_tag="VM841_13835"
     CDS             complement(2318..3274)
                     /locus_tag="VM841_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL91309.1"
                     /translation="MRRAACIVIAIAGLIPVPARAATVGTTFPPGFAVPADATLGVPV
                     IGFGAAGAVTRTPVIFLHGNNDTPYPTTCNGAFGKIQQFAQFFLDNGYAASELWGLGY
                     QGEQCDSLTYPPTKSSDAHSTVANVPDLRAFVAAVLDYTGATQVDIVGHSLGTTLARE
                     WMRQDGAHGLVRRLVGVDGPNHGIINCSPNPLNFWQVPALGGFTPDSGICREYGAVDT
                     PLLSVLNAGDETPGPTEYMTIYNADASFVFFDEQDGLVPPVPAEDRNGNPHDFSASAM
                     LDGAVNVPVVGQGQYDGALLAAHLGIVNSPEVWAIALRRLRE"
     gene            3452..3877
                     /gene="msrB"
                     /locus_tag="VM841_13840"
     CDS             3452..3877
                     /gene="msrB"
                     /locus_tag="VM841_13840"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HVL91310.1"
                     /translation="MKMKKEQDPREVHKSDEEWRRELTPEAFAILRQAGTERAFTGQY
                     WDCHLDGVYRCRGCGAELFDSATKFDSGSGWPSFTEPAVAANVETRRDTSHFMVRTEV
                     VCKRCGGHLGHVFDDGPEPSGLRFCINSAALSLDERAPE"
     gene            3949..4368
                     /locus_tag="VM841_13845"
     CDS             3949..4368
                     /locus_tag="VM841_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVL91311.1"
                     /translation="MKNIKDVMTTQAFTLEASSPITEAARIMSERDVGDVLVTKHGEL
                     CGIVTDRDIVVRALAKGKDLETSVGEICSMKLTTLSPNDSVDKAVELMRENAVRRLPI
                     VEDGKPVGMVSLGDLAMDRDPRSALADISAAPPNNDD"
     gene            complement(4425..6191)
                     /locus_tag="VM841_13850"
     CDS             complement(4425..6191)
                     /locus_tag="VM841_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91312.1"
                     /translation="MTRSRILRLVAAALVVALAALPARSLPPGYVADYLVEPRPLCCG
                     NGIALHGDTLLVNHAALDAISAIDVNERTIASVASEDDGDLEELVTPDDLWRDPVTGD
                     IYVTEILTNGVRKIAPDGTRTRVMTGFGDGVAHPNAITGLRRPGEPLRLFISMVLFEP
                     SARTGIWEIDPHGLLPPRLVYGAAGGQTIYGHGMRAPNAMAFGPDGEELFVPESYGGA
                     VWAIDVDAHTARTVFDGASPAANGIALRFAADGSILYAEQNTGRLLRLSPQGPSSQIP
                     QVVAQLDPGIDGIAEHADGRIFLSNFRFGGIAIVHPGGAVEQMFRRGMDLPNGIVEVR
                     EPGGDYDPQPVKTWAVGDLGSLALVDASTGAIDRVKQFIRDDFDIAIGAGTTFGCDVY
                     ATGFFRGTLQKVNVCDPAAPHTEVVPRRTFVGAWDVMGPTLASLFVSDVVTGIVWDVR
                     YQSQPVVFTPFAAGLAGPTGLAWNGGDLVFVAETEADRIRVYRRFDGGLHGILTDFDE
                     PEGLAFFAGRLLVVEAGAGRLTAVSADGTREVLLEGLATKIRGVGAVPVLNYFSDVAV
                     AADGTIVVTSPLDGSIIRLRRP"
     gene            complement(6246..7967)
                     /locus_tag="VM841_13855"
     CDS             complement(6246..7967)
                     /locus_tag="VM841_13855"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HVL91313.1"
                     /translation="MLIAGGCSLRADSKPESVRLFPPPGTAVASPESQITLRGGGAAD
                     VGTVTVTGSASGTHEGRMQAHSDGQGVSFVAEKPFQPGETVTVSAGMKIAGAGESGAT
                     FTVARPAEQKWEPGPTVRKDWEKGALTLKTHPELRPSSITATTPAAKPGKDLLLLTPH
                     GGPGGSGPQIMDDRGNLVWYQAMPSGVSAADLRVQTMAGKPVLTYWKGTVTTGMGRGE
                     YVVLDKTYTEIATIDAGNGYTSDLHDMRLTEKGTAWIMIYTPVKWDLSPAGGPADGIA
                     IDNVVQEIDVATGAVLFEWHSLDHVGVDESVKELPKTPDLGYDYFHINSLDVERGGTL
                     LLSARNTSALYRIDATSGEMVWRLGGKKSDFSMAEGATFGYQHDARQMPDGTIMLFDN
                     AAATEETEGAVSAALRLKLDDDRKTATVVARWTHPTKLLAASRGSVQRLENGNTFVGW
                     GSKPFFTEYDASGNVVFDARMDPGVSYRTYRMPWTGRPAAEPALSVTATPSGVSVWVS
                     WNGDTETATWELLAGDSVTDLKPAGRVTRTGFETELTGAAAAFVAVRALDASGQELIR
                     SQPRAAG"
     gene            8247..11054
                     /locus_tag="VM841_13860"
     CDS             8247..11054
                     /locus_tag="VM841_13860"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVL91314.1"
                     /translation="MPSPIGTLHRGFRRLADWLPRGRTLPDDIWTKRHRGITVILWAH
                     VAFLPIVGFARDYGLVHTLLETGIVAVPAVVGSLPALPRRARTIAASFGLMSTSAVLV
                     HLSGGLIEMHFHFFVMVALVALYHDWIPFLTAIGYVLFHHGVMGTIDPNSVYNHLAAW
                     RNPWLWAGIHALFIAGISLTCLVTWRLNENLLEERRFAEVRLREESAVVEALHAVGRT
                     IAAELEMERLVQDVTDAATELTGAQFGAFFYTQTAADGEPFTLYSLSGAPKSAFEKFP
                     LPRNTAVFAPTFEGKGVVRIDDVHADPRYGKNEPYYGMPKGHLPVRSYLAVPVFARSG
                     EVLGGLFFGHSEVGRFNAVHERIIVGIAAQAAVAIDNARLYELEREARRASDAAGRQV
                     ALLAEMGTVLASSLEIDELVDGAARTIVPTFADVCIIDLVDDDGRLRRAAVTSDPSLA
                     EVADVIRRHPPALENLAHPALRALREGKTQILYPFTDEAMSSIATDEEYVAALTRFRP
                     TAGIVAPLRGRNRLLGTLSVATFEATGRTVGEPEEPVLQELARRLAVALENAHLYARQ
                     RGVAETLQHALLPERLPDIPGLQAAARYIAGGSGVDVGGDWYDVVRLPKGAIALALGD
                     VVGRGERAASLMGQIRNAARAYAMDHKGPAEIAEHLNRLLTDLGHESMATMIFAILDP
                     DSGTLRFVNAGHPPPLVVGPENTAEFLDGDTGVPLGATARARYTETVTTLTPGSTLLL
                     YTDGLIEDRTTMLDDGLERLRQAAMRCGCDPETLCDTALEEAPAGRKGDDDVAMLAVR
                     LVPLGERLHLRLPRDPTLVSSVRSTMRRWLAATGASEQEIYEIVLATGEACANAVQHA
                     VGPARSHFEVRGSREGDSVVIDVWDQGRWRTVRATGGGRGIDIMRAFMDDVVIDRGAT
                     GTRVTMRRRLAGLVMHEATIE"
     gene            11051..11425
                     /locus_tag="VM841_13865"
     CDS             11051..11425
                     /locus_tag="VM841_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL91315.1"
                     /translation="MTDYRLSLGTRDAILVVTLEGEVDFTNVSAIEARILDASSNDLI
                     AIAVDLSKTSYLDSAGVRLLFDLARHTDACRQRLGIVVPDDAPLRRLVTVTNLALVAA
                     VSATEADCLKDLRDDVAGFANG"
     gene            complement(11422..14148)
                     /locus_tag="VM841_13870"
     CDS             complement(11422..14148)
                     /locus_tag="VM841_13870"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HVL91316.1"
                     /translation="MRRTLIAFALLIAALPLHGRSNPVTITPGSPVRAGYGQVDSTWR
                     VGAGAGQYSAKDPNLAHAVQGNQADPYNHSWTQRRSYGVHSRLSFRAIVVEGANGRRV
                     ALVKSDHYLASDALARRAAQILDASGTSGVGYEDILLMASHNHSSPFYYSPSWGPWLF
                     QDLFDLRAFEYEARNFAKAIELAAADLRPARMGATTVEHTIYKGQIARATIGDDGSPA
                     GYPESHADFGLSVLRFEEHPSGTPIAAFVNFGQHPESLDGYDLITADFLAPLERMVER
                     DLGAPLVWSQGDVGSAEGPYFGGNDVLPDGVVRAWAHVGHAQTERGARYLADSIVDAW
                     HRIGRGEGSVPLSPSFPVDMAEAWVPGPVSHPYASVSNCRTEPTVEGNPGSPIVGLPD
                     CGRAGRTDQTNPTWETLKESGVPLPEHYDAPSWGAVEENQRLRLQAVRLGEVVLASCS
                     CEAQMDLILNFESRADDVEGNIFDGYEYPCTQNPNATWTCKAGAKLDLNVTISDAKHK
                     RMVAQIHNDAKGWDDVENAVESNTEPADPAKIRGNFTKQELPASLGYTLPIGVGMAAE
                     YQGYVVSYREYMIRDHYRKALTAYGPHTADYMVTRMVRLAALLKGGPALPDEPLQAHA
                     TVDEMRQTVFTAVLGAMSSATYDAWVAALPNDAWAAAGVQQPVDVTRFQAATFRWRGG
                     SNAVDNPLVRVERKVGGQWVPYADQTGEVQTMVTFPAGADALADSYTGAREWIWTANF
                     EAFDAFPATVIDGGQTPDGEYRFVVQGTRRSGGADVPYHLASDSFLVGPWRGITVSDS
                     RREAGGDVSFVVDPIRYPRTYASGFRYIRDDGNQTLCKTCTFRPWASRGTVASAAVYV
                     DRLVGPDEVVPATLQNGRWVAAAGVLPGDVVTIRPGDVRDAFGEINGSSLTF"
     gene            complement(14167..16671)
                     /locus_tag="VM841_13875"
     CDS             complement(14167..16671)
                     /locus_tag="VM841_13875"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVL91317.1"
                     /translation="MKSRLVVLAGVVALAFPALAAGSPPVQVPGGFRAATINTPGQSG
                     HISVLDFAADTANGTKTYGPHLTDGLVPYTAYKYKPGEFFRSGTPTIPRPGVKIYRDS
                     FGVPAVWGGSIADAWYGVGYALAEDRMWQMHLLRMAALGRLAELIGTGGLPSDIATRR
                     DFYTPAEYAQQFASLDDWEKEQLIAYAAGVNQYIAEMHADPRKMPAEIAALALPIEPW
                     TPIDSFALGGLIARSVASDGGQELGNAALLRDLITTYGATEAAKIFNDVLWRNDPAAP
                     TSVPASEGTFSSYPHGAPLGSSLQRSLSLVEALPGSLPDVAAQLTAERAVRDKLRTEL
                     GLPRPGSNVWAVSPSRSANGNAMLFNGPQVGYTVAGLLTEFEVHGGRGDRRLDAKGIT
                     VAGFPAVGIGYTPHHAWGLTSGLSDTIDMYVEQLAGDKHSYRYDGETRKMQCRTELFK
                     LRNTLAPEQTGIHTQELCRTVHGPVIAIDEAGGVAYAQRYAIWNNEIGTLRSMFRWPY
                     AKNLEEFTDAMRLVTWNENLTYADAEGNIAYWHPGSYPARPRAFDERLPYPGDGSAEW
                     ERLLTFEEMPHTINPAQGWVANWNSKPSAGWTSGDPHYSDRPWGVANRLSALADVLGG
                     PAPIGAIPAPGPDGLIDGTQGLFAPDVAGGNFDLTFPAFRPYLAEARAATSDPRLAAA
                     LDAMLAWDGSHFDANNDGKADTPGHTIFHEWVEQAARGVFNPYLLGHGAFFRGGHRTD
                     PSPVINMFLRALQGPAAALPQSRDYLNGKTRTQVILETLDTRLNALQAIYGSNMNAWL
                     MPAFRSRLESQGLGPGGSFRYQDRGSWIEAIEYFVD"
     gene            complement(16827..18134)
                     /locus_tag="VM841_13880"
     CDS             complement(16827..18134)
                     /locus_tag="VM841_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVL91318.1"
                     /translation="MTRVFEILPGRIERPAAAAAGWLAARGVGGGDRIVVPAQNDPRL
                     LALTQGALRTGIVPVLINPAMDERERSWIESDAEPALIVRDLDQIDWDFGTAELAPRP
                     LGRPMLYTSGTTGRPKGVWGGLLDEADARAWALDEQALWAAAPDAPVLVCSPLYHSAG
                     HRLATAALIAGARILLLERFDADAVVRILSKQAVSSVFLVPTHLRRILQAGDPPAPRD
                     ARKVLHAGESCPAELKRRALDWIENLWEFYGSTEGQFTAISPDEWRLRPGSVGRARAA
                     RTLSIAEAGEDGIGTVFVTAPPFARWEYWRDPQRTAAAWRGGAFTAGDLGRLDDGYLT
                     LAARREDLIITGGVNVYPGMIEAALLEHPGVRDVAVFGVADDQWGQRVCAAIVADEPL
                     EAWIHERLHGPYLPKTVLRMDALPRTPVGKIDRAALRDAIASG"
     gene            complement(18421..>18747)
                     /locus_tag="VM841_13885"
     CDS             complement(18421..>18747)
                     /locus_tag="VM841_13885"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HVL91319.1"
                     /translation="EWLMHFMIAMCDPRRTEGVREVYEIRLPPGTFHVHADDGELRFH
                     DGEAEAPDLVIEADLETFVGLANEDADPAAAVAAGHLRATGDPAAGLRALHILRPATS
                     AAPIRS"
BASE COUNT         2915 a         6491 c         6410 g         2931 t
ORIGIN      
        1 gcgcgtcact ggtgggcgca gcagcacggg gtcgacgtgg acgcggccgc gagcccggcg
       61 aagttcgcgt aggtcgcgct cggcgtaagc gtgccgcccc acgagagcgg aagatcgaaa
      121 aggtcgagca gccacccgat cgaatgctgc tcgagccgcg cgctcagagg cgacgacggc
      181 cacagcgcgc cgttctggtc gatcagcgac gtgagccagt cggcaccgag cgccgcgggc
      241 gtgacaccgc cgatcacgaa gtggaagaag cgcggcccgc tggaagccgt tgccgcggcg
      301 agcccgtcct cgaccagcgc gcgcaccgca cgcatcgatc ccaatcctcg ctcgggaaaa
      361 tcgcgtggga gccgctcccg atgatccgcc gcatcgaaag tgacgaccgg tctcgcgtcg
      421 atcgtggaga ggtatctctt ggctgcatcg gccaccatat cgatgacggc gtcgagatcg
      481 ccacggtcgt cgatgggatc gctcatgccg cgatcctagc gcagcaatga ctccggaagg
      541 tccacgattc tcgcgcgcaa atactcgccc agaccctttg cctgaggatc gatgcgattc
      601 gatgccgcaa cgcccttgcc cagcaggccg tgcaccacga agttgagcgc gcagatgttc
      661 ggcatgtcga accgttcgac gttcagatcg cgcgcttcgg tgatcaactc tttgaacttg
      721 ccgacggtca ggaagccgct gagccattcg aacgccgtcg catcgcgcgt ccacacgccc
      781 aagttcgcgt tgccgccctt gtcgccgctg cgcgcgcctg cgacgcggcc gagcggggca
      841 cgcaccgtcg aaccgtcgtg atcgaagttg cggacgcgcg gcggcgcggg cggctcgtcg
      901 aacgtttcgg ggatcgcgac cggctcgacg gcgtggcgct cgccgtcgat cacgacggtc
      961 tgcgagacga gctcgttcgg gatcagcacc ggcatgtaca cgccgtaggg tgatccctcg
     1021 tcgggcagcg aggtgccgaa gaagccggga taggtcgcca gcgccatttc gatggccgcg
     1081 ttgccgaacg ccttgcgccc gaccttctgc ggatcggagt cgatcaccgt gaccttcaga
     1141 taggcgagcg cctgctcgtt ggtgcgcgga tcgtcgtggt cggtgcgaag cagctgcacg
     1201 tccacttccc tgaacgcgtc cttgccgccc gggatcgacg cccacagcgt cgcctccgcc
     1261 acggccgcct tcttgtcgat gtcgaggccg gtgagcacga acgtgaacga gttgcggaag
     1321 cccccgagcg tgttgacccc gaccttggtc gtgggcggcg gcggctcacc cttgacgccg
     1381 gagatccgca cgcggtccgc gccgtcctgc gccagctcga tggtatcgaa gcgcgccgtc
     1441 acgtccgggt tgtggtaacg gggcgaggcg acctcgtaga cgagctgcgc ggtgacggtg
     1501 cccaccgaga ccagcccgcc ggtgccggcg tgcttggtga tgacgctcga tccgtcctca
     1561 gagacctcgg cgatcggaaa gccgacgtgt tcgatgccgg gaacctcttc gaagaacgcg
     1621 tagttgccgc cggtcgcctg cgcgccgcac tcgatcacgt gaccggcgac gaccgcgccc
     1681 gcgagccggt cccagtcgtc gcgcttccag ccgaacctcc acgcggccgg accgacgacg
     1741 agcgccgcgt cggtcgagcg gcccgtcacc accacgtcgg cgccgcgctc gagcgcgtcg
     1801 gcgatccccc acccgccgat gtaggcgttg gcggtgatcg ggtgcacgcc gtcgagcggc
     1861 tcgccggtgt ccgcgttgcg aagctcgacg cccgcggccc gaaactcgtc gatgcgcgac
     1921 agcacgtcgt cgccttcgac gtgcgcgaca cgcgggctca ggccgagctg ttcggcaagc
     1981 gcgcgaacgg cgtcggcgca gccggccggg ttgagcccgc ccgcgttcga cacgatcttg
     2041 atgccgcgat cgaggcaggt gcccaggacg tcttcgagct gcgtcaggaa cgtgcgcgcc
     2101 cagccggcct cggggttctt catcttgctg cgcgcaagga tcagcatggt cagctcggca
     2161 agccagtcgc ccgagagcac atcgatgtgg ccgccttcga cctgctcgcg cgccgcggcg
     2221 aggcgatcgc cgaagaagcc gctgcagttg gcgatgacga ttggttcgct catgggcggc
     2281 agtatgcaac gagggcgggc acccgcacga atccgcgcta ctcgcgcagc cggcgaagtg
     2341 cgatcgccca gacctccggc gagttcacga tgcccaggtg cgcggccagc agcgccccgt
     2401 cgtactgccc ctgccccacg acggggacgt tgaccgcgcc gtccagcatc gcgctggcgg
     2461 agaagtcgtg cgggttgccg ttgcggtcct cggccgggac gggcggcacc aggccgtcct
     2521 gctcgtcgaa gaacacgaag ctggcgtcgg cgttgtagat cgtcatgtac tcggtggggc
     2581 ccggcgtttc gtccccggcg ttgagcaccg acagcagcgg cgtgtcgacg gcgccgtact
     2641 cccggcagat gccgctgtcg ggcgtgaaac cgccgagcgc gggaacctgc cagaaattca
     2701 gcgggttcgg cgagcagttg atgatgccgt ggttgggacc gtccacgccc accagccggc
     2761 gcaccaggcc gtgggcaccg tcctgtcgca tccattcgcg cgcgagcgtg gtgccgaggc
     2821 tgtgcccgac gatgtcgacc tgcgtggcgc ccgtgtaatc gagcacggcg gccacgaacg
     2881 cgcgcaggtc gggcacgttc gcgacggtcg agtgggcgtc gctcgacttc gtcggcgggt
     2941 acgtcagcga atcgcactgc tcgccctggt agcccaggcc ccacagctcg ctcgccgcgt
     3001 agccgttgtc gaggaagaac tgagcgaact gctggatctt tccgaacgcg ccgttgcagg
     3061 tggtcggata cggcgtgtcg ttgttgccgt gcaggaagat caccggcgtg cgcgtcaccg
     3121 ccccggcggc cccgaacccg atgaccggca cgcccagcgt cgcgtcggcc gggacggcga
     3181 agccgggcgg aaacgtggtg ccgaccgtgg cggcgcgcgc gggcaccgga atcaggccgg
     3241 cgatggcgat cacgatgcaa gcggcacgtc tcatgagcgg ctcccggctc gcggtccaca
     3301 aacctgaatc gagtctcagg tcttgtccct tttgtacctt tgctggagcg ccgggtcaag
     3361 ccccgctccc gccggcgtcg cgagacgcgg cccggctcgg gcaccgatcc gacggcgcgc
     3421 ggcgttcata tgctggacca aggaggatcg catgaagatg aagaaggaac aggacccccg
     3481 cgaggtgcac aagagcgacg aggagtggcg gcgcgagctc acgcccgagg cgttcgcgat
     3541 cctgcgtcag gccgggacgg agcgcgcgtt caccggccag tactgggatt gccacctcga
     3601 cggcgtgtac cggtgtcgcg gatgcggcgc cgagctgttc gactccgcga cgaagttcga
     3661 ctcgggttcg ggctggccgt cgttcaccga accggccgtc gcggccaatg tcgaaacgcg
     3721 tcgtgacacg agccacttca tggtgagaac agaggttgtg tgcaagcgat gcggcgggca
     3781 tctcggacat gtcttcgacg acggccccga accgagcggc ttgcggttct gcatcaactc
     3841 cgcagcgctc agcctcgacg agcgagcgcc cgaataaaag cgctcgctcg catcgtttcg
     3901 gcctgcaagc acgccgggta tcgcactcag tgaaggaggg agagcgagat gaagaacatc
     3961 aaagatgtga tgaccacgca ggccttcacg ctcgaggctt cttctccgat cacagaagcc
     4021 gcgcgcatca tgagcgagcg tgacgtgggt gacgtgctcg tgaccaagca cggtgagctg
     4081 tgcggaatcg tcaccgatcg cgacatcgtc gttcgcgcgc tcgccaaggg aaaggacctc
     4141 gaaacgtcgg tcggtgagat ctgctcgatg aagctcacca cgctgtcccc gaacgattcg
     4201 gtcgataaag ccgtggagtt gatgcgagaa aacgccgtgc ggcgcctgcc catcgtggag
     4261 gacggcaaac ccgtcggcat ggtgtcgctc ggcgacctcg ccatggaccg cgacccacga
     4321 tcggcgctcg ccgacatcag cgcggctccg ccgaacaacg acgactgaac gcctccgtcc
     4381 gaacgctcgc cggtcctgca cgggaccggc gagcggcttt tcggctacgg acgccgaagc
     4441 cgaatgatcg agccgtccag cggcgaggtg accacgatgg tgccatcggc cgcgaccgca
     4501 acgtcggaga agtagttcag caccgggacc gcacccaccc ctcggatctt ggtcgcaagc
     4561 ccctcgagca acacctcgcg cgtgccgtcg gcgctcaccg cggtgagccg cccggctccg
     4621 gcctcgacca cgaggagccg gccggcgaag aacgcgaggc cttccggctc gtcgaagtcc
     4681 gtcaagatgc cgtgcaggcc tccgtcgaat cgacggtaca cccgaatccg gtccgcttcg
     4741 gtttcggcca cgaacaccag gtccccgccg ttccacgcga ggcccgtcgg tcccgccagg
     4801 cccgcggcga aaggcgtgaa gacgaccggc tgcgactgat agcgaacgtc ccacacgatc
     4861 ccagtcacga cgtcgctgac gaacaggctc gcaagcgtcg ggcccatcac gtcccacgcg
     4921 ccgacgaacg tgcgccgtgg caccacttcg gtgtgcggcg ccgccggatc gcacacgttc
     4981 accttctgca aggtcccgcg gaagaacccg gtcgcgtaca cgtcgcaccc gaacgtggtg
     5041 ccggcgccga tcgcgatgtc gaagtcgtcg cggatgaact gcttgacccg gtcgatggcg
     5101 ccggtgctcg cgtccacgag cgcgagcgat cccagatccc cgaccgccca ggtcttgacc
     5161 ggctgcgggt cgtagtcccc tcccggctcg cgcacctcga cgatgccgtt gggcagatcc
     5221 atgccgcgcc ggaacatctg ctcgaccgct ccgccgggat gcacgatcgc gatgccgccg
     5281 aagcggaagt tcgaaaggaa gatgcggccg tcggcgtgct cggcgatccc gtcgatcccc
     5341 ggatcgagct gcgccacgac ctgagggatc tgcgacgacg gaccctgcgg ggacagacga
     5401 agcaaacgcc cggtgttctg ctccgcgtac aggatcgagc cgtcggcggc gaagcgaagc
     5461 gcgatgccgt tggcggccgg tgacgcgccg tcgaagaccg tgcgagcggt gtgcgcgtcc
     5521 acgtcgatcg cccagaccgc gccgccgtag ctttccggga cgaacagctc ctcgccgtcc
     5581 gggccgaagg ccatcgcgtt gggcgcgcgc atgccgtggc cgtagatcgt ctggccgcct
     5641 gcggcgccgt acacaaggcg cggcggcagc agcccgtggg gatcgatttc ccagatgccg
     5701 gtgcgtgccg agggctcgaa caagaccatg ctgatgaaca gccgcagcgg ctcacccgga
     5761 cggcgcaggc ccgtgatcgc gttcgggtgc gcgaccccgt ccccgaagcc cgtcatcacc
     5821 cgcgtgcgcg tcccgtcggg cgcgatcttg cgcaccccgt tggtcaggat ctcggtcacg
     5881 tagatgtcgc cggtcaccgg atcgcgccac aggtcgtcgg gcgtgacgag ctcctcgagg
     5941 tcgccgtcgt cctccgaggc gaccgaggcg atcgtgcgct cgttgacgtc gatcgcgctg
     6001 atcgcgtcga gcgccgcatg gttgaccagc aacgtgtcgc cgtggagcgc gatgccgttt
     6061 ccgcagcaca acggccgcgg ctcgaccaga tagtcggcca cgtagcccgg cggaagcgag
     6121 cgcgccggaa gcgccgccag cgcgacgacc agcgcggctg ccacgagccg aaggatccga
     6181 gaacgagtca tggcgatcat ttctccgcga ccgccgccga gtcctgtcgg gcgtctcccc
     6241 ggcgtctacc cggctgcccg gggctgcgac cggatgagct cctgaccgct cgcgtccagc
     6301 gcccggaccg cgacgaaggc ggccgccgcg cccgtgagct cggtctcgaa cccggttcgc
     6361 gtcacgcgtc cggccggctt caggtcggtg accgagtctc cggcgagcag ctcccacgtc
     6421 gcggtctcgg tgtcgccgtt ccaggacacc cagaccgaca ctcccgacgg cgtggccgtg
     6481 accgaaagcg ccggctcggc ggccggccgg cccgtccacg gcatccgata ggtgcgatac
     6541 gacacgcccg gatccatgcg agcgtcgaac acgacgttgc ccgaggcatc gtactcggtg
     6601 aagaacggct tgcttcccca gccgacgaac gtgttgccgt tctccaagcg ctggacgctt
     6661 ccacgcgagg ccgcgagcag cttcgtcgga tgcgtccagc gggccaccac cgtcgccgtc
     6721 tttctgtcgt cgtcgagctt gagacgcagc gccgcggaca ccgcgccctc ggtttcctcg
     6781 gtcgcggccg cgttgtcgaa gagcatgatg gttccgtcgg gcatctggcg cgcgtcgtgc
     6841 tggtagccga acgtcgcgcc ctcggccatc gagaagtcgc tcttcttgcc gcccaggcgc
     6901 cagaccatct cgccgctcgt cgcgtcgatc cggtacagcg cgctggtgtt gcgcgccgac
     6961 agcagcagcg tgccgccgcg ctcgacgtcg agggagttga tgtggaagta gtcgtatccc
     7021 aggtcgggcg tcttcggcag ctccttcacc gattcgtcga cgccgacgtg gtcgaggctg
     7081 tgccattcga acagcaccgc gccggtcgcg acgtcgatct cctgaacgac gttgtcgatc
     7141 gcgatgccgt cggccggccc gccggccggg ctcaggtccc atttcaccgg cgtgtagatc
     7201 atgatccatg cggtgccctt ctcggtcagg cgcatgtcgt gcaggtcgct ggtgtagccg
     7261 ttgccggcgt cgatcgtggc gatctcggtg taggtcttgt cgagcaccac gtactcgccg
     7321 cggcccatgc cggtcgtgac ggtacccttc cagtaggtca ggaccggctt gcccgccatc
     7381 gtctggaccc gcagatctgc ggcggacacg ccggagggca tcgcctggta ccagaccagg
     7441 ttgccgcgat cgtccatgat ctgcgggccg gagccgccgg gtccgccgtg cggggtcagc
     7501 agcagcaggt ccttgcccgg cttggcggca ggggtggtcg ccgtgatcga cgacggccga
     7561 agctcgggat gggtcttgag cgtcagggcg cctttctccc agtccttccg gaccgtcggg
     7621 ccgggctccc acttctgctc ggcgggccgg gcgacggtga aggtggctcc tgactcgccg
     7681 gccccggcga tcttcatgcc cgcggagacc gtaaccgtct cgcccggctg aaacggcttt
     7741 tcggccacga agctgactcc ttggccatcc gaatgcgcct gcatgcgtcc ctcgtgggtg
     7801 ccgctggccg agccggtgac cgtgacggtc cccacgtccg ccgccccgcc cccgcgcagc
     7861 gtgatctgtg attcgggcga ggcgaccgcc gttcccggcg gcgggaagag tcgcaccgac
     7921 tcgggcttcg agtcggcgcg cagggagcag ccccccgcga tgagcatcaa ggcgatcccg
     7981 gcggcgaacc ctcgcgcggc cgcgatcccc gaacgtcgtt ggaaagatcg acccctggtt
     8041 gtcatggggt caaacggtac taaatcgcac cagcctatgg ttaatctaac ccaaagaatc
     8101 gcgcgtggag cccggagcta cccgaagggt ctggacggtc gccccccggg gattatggcc
     8161 gggggatttt gggaacaagg tgtcgtacgc gcgcggcgca tggttcgccg tgcccggggg
     8221 ggtaggggag tttggacgca gcgtcggtgc cgagtccgat cggaacgctc catcgagggt
     8281 tccgccgcct tgccgattgg ctccctcgcg ggcggaccct gcccgacgac atctggacca
     8341 agcgtcatcg aggcatcacc gtaatcctat gggcgcacgt cgcgttcctc ccgatcgtag
     8401 gtttcgcccg cgattacggc ctggtgcaca ccctcctcga gaccggcatc gtggcggtgc
     8461 cagcggtggt gggctctctc ccggccctgc cgcggcgggc ccggaccatc gccgccagct
     8521 tcgggctcat gagcacctcg gccgtcctgg tgcatctgtc gggcggcctg atcgagatgc
     8581 actttcactt cttcgtaatg gtcgccctcg tggcgctcta ccacgactgg atcccgttcc
     8641 tgaccgccat cggctacgtc ctgttccatc acggcgtgat ggggaccatc gacccgaact
     8701 ccgtctacaa ccatctggcg gcctggcgga acccatggct gtgggccggc atccatgcat
     8761 tgttcatcgc cggcatcagc ctgacgtgcc tggtcacctg gcgactcaac gaaaatcttc
     8821 tcgaagagcg ccgcttcgcc gaggtgcggc tgcgggagga gtcggccgtg gtcgaggcgc
     8881 tgcacgcggt cggccgcacc atcgcggccg agctcgagat ggagcgcctc gtgcaagatg
     8941 tcaccgacgc cgcgaccgag ctgaccggcg cgcagttcgg cgcgttcttc tatacccaga
     9001 ccgccgccga cggcgagccc ttcacgctgt attcgctttc gggcgctccg aagtcggcgt
     9061 tcgagaagtt tcctttgccg cgcaacaccg cggtgttcgc gcccacgttc gaaggcaaag
     9121 gcgtggtccg catcgacgac gtgcacgcgg acccgcgcta cggcaagaac gagccttact
     9181 acggcatgcc caaagggcat ctgcccgtgc gcagctatct cgcggtgccc gtctttgccc
     9241 gcagcggcga ggtgctcggg ggacttttct tcggacattc cgaggtgggg cgtttcaacg
     9301 cggtgcacga gcgcatcatc gtgggcatcg ccgctcaggc ggccgtcgcc atcgacaacg
     9361 cgcgcctgta cgagctcgag cgcgaggcgc ggcgcgcgtc agacgcggcc ggccggcagg
     9421 tcgcccttct tgccgagatg ggcacggtcc tggcctcgtc cctggagatc gacgagctcg
     9481 tcgacggcgc cgcacgcacc atcgtcccga ccttcgccga cgtgtgcatc atcgatctgg
     9541 tggacgacga cggccggctg cggcgcgccg ctgttacgtc ggatccgtcg ctcgccgagg
     9601 tcgccgacgt gatccgccgc cacccccccg cgctcgagaa cctcgctcat ccggccctgc
     9661 gagcgctccg ggaaggaaag acgcagatcc tgtacccctt caccgacgaa gcgatgtcat
     9721 caatcgcgac cgacgaggag tacgtggccg cgctcacccg gttccgcccg acggcgggta
     9781 tcgttgcgcc gcttcgcggc cgcaaccgcc tgctcggcac gctatcggtc gcgaccttcg
     9841 aggccaccgg ccgcacggtc ggcgagccgg aagagcccgt gctgcaggag ctcgcacggc
     9901 ggctcgccgt cgcgctcgag aacgcgcatc tctacgcgcg ccagcgaggc gttgccgaga
     9961 cactccagca cgcgctgctg cccgagcggc tccccgacat tcccggcctc caggcggccg
    10021 ctcgctatat cgcgggcggc tcgggcgtgg acgtcggcgg agactggtac gacgtggtgc
    10081 ggctgcccaa gggcgcgatc gcgctcgcgc tcggcgacgt ggtcggccga ggcgaacgcg
    10141 ccgcatcgct gatgggtcag atccggaacg cggcccgcgc ctacgcgatg gaccacaagg
    10201 gcccggccga gatcgccgag catctcaacc ggctgctcac ggacctgggg cacgagagca
    10261 tggccacgat gatcttcgcg atcctcgacc ccgactcggg cacccttcgc ttcgtcaacg
    10321 cgggccatcc accgccgctg gtcgtcggac ccgagaacac cgccgagttc ctcgacggcg
    10381 acacgggcgt gcccctgggt gcgacggcgc gcgcccgcta taccgaaacg gtgacgaccc
    10441 tgacccccgg ctcgacgctt ctgctctaca ccgacggctt gatcgaggac cgcacaacga
    10501 tgctcgacga tggactcgaa cggctgcggc aggccgcgat gcggtgcgga tgcgatcccg
    10561 agacgctgtg cgacacggcc ctggaggaag cgcccgcggg acgcaagggc gacgacgacg
    10621 tggcgatgct cgcggtccgg ctggttccgc tcggagaacg cctccacctt cggctccccc
    10681 gcgatcccac gctcgtgtcg tcggtgcgct cgaccatgcg gcgctggctc gcggccacgg
    10741 gcgcgagcga gcaagaaatt tacgagatcg tgctcgccac gggcgaggcg tgcgcgaacg
    10801 cggtgcagca tgccgtcggg cccgcccgct ctcatttcga ggtgcgcggc agccgcgaag
    10861 gagacagcgt cgtcatcgat gtctgggatc aaggccgctg gcgtaccgtg cgcgccaccg
    10921 gcggcggccg gggaatcgac atcatgcggg cgttcatgga cgacgtggtg atcgaccggg
    10981 gcgcgaccgg aactcgcgtg accatgcgcc gccggcttgc cggcctggtg atgcacgagg
    11041 cgacgatcga atgaccgact accgcctgtc gctgggcaca cgagacgcca ttctcgtcgt
    11101 gaccctcgaa ggcgaggtcg acttcaccaa cgtctcggcc atcgaggcca ggatcctgga
    11161 cgcgtcgtcc aacgacctga tcgcgatcgc ggtcgatctt tccaagacca gctacctcga
    11221 cagcgcgggc gtccggctgc tgttcgatct ggcccgccac accgacgcgt gccggcagcg
    11281 gctgggcatc gtcgtgcccg acgacgcgcc gcttcggcgt ctggtcacgg tcaccaacct
    11341 cgccttggtc gcagccgtat ccgcgaccga ggccgattgc ctgaaggacc tgcgcgacga
    11401 cgtcgccggc ttcgcgaacg gctagaacgt cagcgacgat ccgttgatct cgccgaaggc
    11461 gtcccgcaca tcgccgggcc tgatcgtcac cacgtcaccg ggcagcacgc ccgcagcggc
    11521 cacccatctc ccgttctgca gggtcgcggg cacgacctcg tccggcccca cgagccggtc
    11581 cacgtacacg gccgccgacg caaccgtccc gcgcgaggcc cagggccgga acgtgcaggt
    11641 cttgcacagc gtctggttgc cgtcgtcgcg gatgtagcgg aagcccgacg cgtacgtgcg
    11701 cggataccgg atcggatcca ccacgaacga cacgtcgccg cccgcttcgc gtcgcgagtc
    11761 gctcacggtg atgccgcgcc acggcccgac gaggaacgag tccgacgcga ggtggtacgg
    11821 cacgtccgcg ccgccgctgc ggcgcgtgcc ctgcacgacg aaccggtact cgccatcggg
    11881 cgtctggccg ccgtcgatca ccgtggccgg gaaggcgtcg aaggcctcga agttcgccgt
    11941 ccagatccac tcgcgggcgc cggtgtagct gtcggcgagc gcgtcggcgc cggcggggaa
    12001 ggtcaccatc gtctggacct caccggtctg gtccgcgtag ggaacccact ggccgccgac
    12061 cttgcgctcc acgcgcacga gcgggttgtc caccgcgttc gagccgccgc gccagcggaa
    12121 cgtcgccgcc tggaagcggg tcacgtcgac gggctgctgg acgcccgcgg ccgcccatgc
    12181 gtcgttggga agcgccgcga cccacgcgtc gtaggtcgcc gaggacatcg cgccgagcac
    12241 cgcggtgaag acggtctgac gcatctcgtc cacggtggcg tgcgcctgca gcggctcgtc
    12301 cggcagcgcc ggaccgccct tgagcaacgc ggcgagccgc accatccggg tgaccatgta
    12361 gtcggcggtg tggggcccgt aggcggtgag ggccttgcga tagtgatcgc ggatcatgta
    12421 ctcgcgatag ctcacgacgt agccctgata ctcggccgcc atgcccacgc cgatcggcag
    12481 ggtatagccg aggctcgcgg gaagctcctg cttggtgaag ttgccgcgga tcttggccgg
    12541 atcggccggc tcggtgttgg actcgacggc gttctcgacg tcgtcccatc ccttcgcatc
    12601 gttgtggatc tgcgcgacca tccgcttgtg cttcgcatcg ctgatcgtga cgttgaggtc
    12661 gagcttggcc cctgccttgc acgtccaggt cgcgttgggg ttctgggtgc acggatattc
    12721 gtagccgtcg aagatgttgc cttcgacgtc gtcggcgcgg gactcgaagt tcaggatcag
    12781 gtccatctga gcctcgcaag agcacgacgc gagcacgacc tcgccgagcc gcacggcctg
    12841 cagtcggagc cgctggttct cctcgaccgc cccccatgag ggcgcgtcgt agtgctcggg
    12901 cagcgggacg cccgactcct tcagcgtctc ccacgtcggg ttggtctggt cggtacggcc
    12961 cgcgcggccg cagtcgggca gccccacgat cggcgagccg gggttgcctt ccacggtcgg
    13021 ttcggtccgg cagttcgaca cggacgcgta ggggtgcgag acggggccgg gcacccaagc
    13081 ctcggccatg tccaccggga acgacggcga cagcggaacc gacccttcgc cgcggccgat
    13141 cctatgccac gcatcgacga tcgaatcggc gaggtatcgc gcgccgcgtt cggtctgggc
    13201 gtgacccacg tgcgcccagg cacgcacgac gccatccggc agcacgtcgt tgccgccgaa
    13261 gtacggccct tcggcgctgc ccacgtcgcc ctggctccag accagcggcg cgcccaggtc
    13321 gcgctcgacc atgcgctcca gcggcgccaa gaagtcggcc gtgatcaggt cgtagccatc
    13381 gagcgactcg ggatgctggc cgaagttgac gaacgcggcg atcggcgtgc cactcggatg
    13441 ctcctcgaat cgaagcacgc tcaagccgaa gtcggcatgc gattcgggat agccggccgg
    13501 cgagccgtcg tcaccgatcg tcgcgcgcgc gatctgaccc ttgtagatcg tgtgctccac
    13561 cgtcgtggcc cccatgcgcg cgggccgcag gtccgcggcg gcaagctcga tcgccttggc
    13621 gaagttgcgg gcctcgtact cgaaggcgcg caggtcgaac aggtcttgga acagccacgg
    13681 cccccacgac ggcgagtagt agaacggcga cgagtggttg tggctggcca tgagcaggat
    13741 gtcctcgtag ccgacgcccg aggtgccgga cgcgtcgagg atctgcgccg cgcggcgcgc
    13801 gagcgcatcc gaggcgaggt agtggtcgga cttcacgagt gcgacgcgcc gcccgttggc
    13861 gccttcgacg acgatcgcgc ggaatgacag ccgcgagtgc acgccgtagg agcgacgctg
    13921 cgtccaggag tggttgtagg gatcggcctg gttgccttgg acggcgtgcg cgagattggg
    13981 atccttggcc gagtactggc ccgcaccggc gcccacgcgc caggtcgagt ccacctggcc
    14041 gtatccggca cggaccggag agccgggcgt gatcgtgacc gggttcgaac gcccgtgcag
    14101 gggcaaggcc gcgatcagca gcgcgaaggc gatgagcgtg cgacgcatca caagaattcc
    14161 ctttcgttag tcgacgaagt actcgatggc ttcgatccac gagccgcgat cctgataacg
    14221 gaacgatccg cccggaccca gaccctgcga ttccagacgc gagcggaatg cgggcatcag
    14281 ccacgcgttc atgttcgatc cgtagatggc ctgcagcgcg ttcagccgcg tgtcgagcgt
    14341 ttcgaggatg acctgcgtcc gcgtcttgcc gttcaggtag tcgcggctct gcggaagcgc
    14401 ggcggccggg ccctgcagcg cccgcaggaa catgttgatc accggcgacg gatcggtccg
    14461 gtgaccgccg cggaagaacg cgccgtgccc gagcaggtag gggttgaaca cgccgcgcgc
    14521 ggcttgctcg acccattcgt ggaagatggt gtggccgggc gtgtcggcct tgccgtcgtt
    14581 gttcgcgtcg aaatggctgc cgtcccatgc gagcatcgcg tccagcgcgg ccgccagccg
    14641 cggatcgctc gtcgcagcgc gcgcctcggc cagatacggc cggaacgcag ggaaggtcag
    14701 gtcgaagttt ccgcccgcga cgtcgggtgc gaacaggcct tgcgtgccgt cgatcagccc
    14761 gtcggggccg ggcgccggga tcgcgccgat cggcgccggg ccgcccagca cgtcggccag
    14821 agcgctgagc cggttcgcca caccccacgg ccggtcgctg tagtggggat cgccgctggt
    14881 ccatccggcc gacggcttcg agttccagtt cgccacccat ccttgcgccg ggttgatcgt
    14941 gtgcggcatc tcttcgaagg tcagcagccg ttcccattcc gccgagccgt caccgggata
    15001 cggcaggcgc tcgtcgaagg cgcgtggccg agccgggtac gaaccgggat gccagtacgc
    15061 gatgttgccc tcggcgtcgg cgtaggtcag gttctcgttc caggtgacca ggcgcatcgc
    15121 gtcggtgaac tcctccaggt tcttggcgta cggccagcgg aacatcgagc gcagcgtgcc
    15181 gatctcgttg ttccagatcg cataccgctg ggcgtaggcg acgccgccgg cttcgtcgat
    15241 cgcgatcacc gggccgtgca cggtgcggca cagctcttgg gtgtggatgc cggtctgctc
    15301 gggcgcgagc gtgttgcgca gcttgaagag ctcggtgcgg cactgcatct tccgcgtctc
    15361 gccgtcgtag cgatacgaat gcttgtcgcc ggcgagctgc tcgacgtaca tgtcgatcgt
    15421 gtcgctcagg ccgctggtca aaccccacgc gtgatgcggg gtgtagccga tgccaacggc
    15481 cgggaatccg gcgacggtga tgcccttggc gtccagacgg cggtcgccgc ggccaccgtg
    15541 cacctcgaac tcggtcagca agcccgcgac ggtgtagccg acctgcggac cgttgaacag
    15601 catcgcgttg ccgttggcac tgcgcgaagg cgagaccgcc cacacattgc tccccggccg
    15661 cggcaggccc agctcggtcc gcagcttgtc ccgtacggcg cgctcggccg tcaattgtgc
    15721 ggcgacatcg ggaaggctgc ccggcagagc ctcgaccagc gacagcgaac gctgcagcga
    15781 gctccccagc ggcgcaccgt gcgggtagga cgagaatgtg ccttccgaag ccggcaccga
    15841 ggtcggcgcc gccgggtcgt tgcgccacag cacgtcgttg aaaatcttcg ccgcctcggt
    15901 cgcgccgtag gtcgtgatca agtcgcgcag cagcgcggcg ttgccaagct cttgaccgcc
    15961 gtcgctggcg acgctgcgcg cgatcaggcc gcccagcgcg aacgaatcga tcggcgtcca
    16021 cggctcgatc ggcagcgcca gcgccgcgat ctcggcaggc atcttgcgcg ggtcggcgtg
    16081 catctcggcg atgtactggt tgacgccggc cgcgtacgcg atcagctgct ccttctccca
    16141 gtcgtcgagc gaggcgaact gctgtgcgta ctcggccggg gtgtagaaat cgcggcgggt
    16201 cgcgatgtcc gagggtaggc cgccggttcc gatcagctcg gccaggcgtc cgagcgccgc
    16261 catgcgcagc aggtgcatct gccacatgcg gtcctcggcc agcgcgtagc cgacgccgta
    16321 ccacgcgtcc gcgatcgaac cgccccacac ggccggcacg ccgaacgagt cgcgatagat
    16381 cttcacgccg ggacgcggga tcgtgggggt gccgctgcga aagaactcac ccggcttgta
    16441 cttgtaggcc gtgtacggca ccagcccgtc ggtcaggtgc ggaccgtagg tcttcgtgcc
    16501 gttcgcggtg tcggccgcga agtcgagcac gctgatgtgg cccgactggc cgggcgtgtt
    16561 gatcgtggcc gcccggaagc cgccggggac ctgaacgggc ggcgagccgg ccgcgagcgc
    16621 gggaaacgcg agcgcgacga cgccggccag aacgacgagc cgtgatttca tagcgagcag
    16681 cccccaggga atcgctttgt ccgattcgcc aagaattcga ctcgatcggc ccgggctcct
    16741 tcccgacagc gaggcgaatc ggccctgtgg accccgaatc cggtccggcc gtcgatttcg
    16801 gacgtaccgg acgcacgagg tgccgcctag cccgaagcaa tcgcgtcgcg cagggcggcg
    16861 cggtcgatct ttcccacggg cgtgcgggga agcgcatcca tgcgcagcac cgtcttcggc
    16921 agatacgggc cgtgcagccg ttcgtggatc catgcctcga gcggctcgtc ggcgacgatc
    16981 gcggcacaca cgcgctgacc ccattgatcg tcggcgacgc cgaacaccgc aacgtcgcgc
    17041 acgcccggat gctccagcag cgcggcttcg atcatccccg gatacacgtt gacgcctccg
    17101 gtgatgatca gatcctcgcg gcgcgcggcc agcgttaggt atccgtcgtc gagccggccg
    17161 agatcgccgg cggtgaacgc gccgccgcgc caggcggccg ccgtgcgctg cggatcgcgc
    17221 cagtactccc agcgtgcgaa cggcggcgcg gtgacgaaca cggttccgat cccgtcttcg
    17281 cccgcctcgg cgatcgacag cgtccgtgcg gcgcgcgccc ggccgacgct gcccggccgc
    17341 aaccgccatt cgtccggcga gatcgcggtg aactgtcctt ccgtcgagcc gtagaactcc
    17401 cagaggtttt cgatccaatc aagcgcgcga cgcttcagct ccgccgggca ggattcaccg
    17461 gcgtgcaaga ccttgcgcgc atcacgcggg gcgggcggat cgcccgcctg gaggatgcgt
    17521 cgcaggtggg tcggcaccag aaacaccgac gagacggcct gcttcgaaag gatccgaacg
    17581 accgcatcgg catcgaagcg ctccagcaga agaattcgag cgccggcgat gagcgccgcg
    17641 gtcgcaagcc ggtgaccggc cgagtgatac agcggcgagc agaccagcac gggggcgtcc
    17701 ggcgcggccg cccatagcgc ttgctcgtcg agcgcccatg cgcgcgcatc ggcttcgtcg
    17761 agcagcccgc cccacacgcc tttgggccgt ccggtcgtgc ccgaggtgta gagcatcggc
    17821 cggccgagtg gtcgcggcgc gagctcggcc gtgccaaaat cccagtcgat ctgatccaga
    17881 tcccgaacga tcaaggcggg ttcggcgtcg gattcgatcc atgatcgctc gcgctcgtcc
    17941 atcgcgggat tgatcaacac cggcacgatg ccggtgcgaa gcgcgccctg ggtcagtgcc
    18001 agcaatcgtg gatcgttttg cgcgggaacc acgatgcgat cgccgccgcc gacgccgcgc
    18061 gcggcgagcc agccggcggc cgccgcggcc ggccgctcga tgcggccggg aaggatctcg
    18121 aatacgcgcg tcatgacgcg ccaggatatc ggccggatct ttgaaaaagt ggggggccac
    18181 gaaccgcgtt ggcggaacgg ctagacagtg acctcgttcg ccttgcggaa ccgcttgaac
    18241 gcggcgaaca gactggccgc cgccgcgacc atcgcggcaa tcgctccgat cttgcgcatc
    18301 gtcatcctga aatcacctct ctccagaggt tccctgctca cacgggtcga aacaccgtca
    18361 agcaaggaac gcggcccgcg gacgcgctcg aacgatctcg atgcccgccg gcgctcgcgg
    18421 tcagctgcga ataggcgcag cactcgtggc ggggcgcagg atgtgcaggg cgcgcaggcc
    18481 ggcggcggga tcgccggtgg ctcgcaggtg gccggcggcg acggcagccg cggggtcggc
    18541 gtcctcgttc gccagaccga cgaacgtctc cagatcggct tcgatcacca gatcgggcgc
    18601 ctccgcctcg ccgtcgtgga agcgaagttc gccgtcgtcg gcgtggacgt ggaaggttcc
    18661 cggcggaaga cggatctcgt agacctcgcg cacgccttcg gtgcggcgcg gatcgcacat
    18721 cgcgatcatg aagtgcatga gccactc
//
LOCUS       DATLFO010000345         6353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_78501, whole genome shotgun sequence.
ACCESSION   DATLFO010000345 DATLFO010000000
VERSION     DATLFO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78501"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1016
                     /gene="larB"
                     /locus_tag="VM841_13890"
     CDS             324..1016
                     /gene="larB"
                     /locus_tag="VM841_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HVL91320.1"
                     /translation="MDHIAALRARRRLDDAHARLDLHRQARTGMPEVIYSPGKTVVQM
                     RSIARVLLERSDAPVIATKATREMFSAVRDLSPTAAYNETARTIVLRREHGSPIGRCM
                     VVTAGTSDIPVAEEAAVTADACGAKVERVFDVGVAGIHRILGVSDDLRTADVIIVAAG
                     MEGALPSVVGGLVQAPVVAVPTSVGYGASFGGIAALLGMLNSCSPGVAVVNIDNGFGA
                     AAFAVRMLRARA"
     gene            1013..2197
                     /gene="larC"
                     /locus_tag="VM841_13895"
     CDS             1013..2197
                     /gene="larC"
                     /locus_tag="VM841_13895"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HVL91321.1"
                     /translation="MSTVAYFDCFSGVAGDMTLGALIDAGVPLDEIEAGLAKLPIEPF
                     ELEVTRVERLGISAIGVRVRAADTGVLRTYAAVRALIEQAELAERPKELALTIFRRLA
                     EAEAKVHGKEVSRVAFHELGSVDTLVDIVGASIALDLLGIEEVHASAVATGMGMIRTD
                     HGIYPVPGPAVVELLKGAPMYSRGIPTELVTPTGAAILAATARSFGDMPQIRVKTVGY
                     GAGTRELEIPNVLRVIVGEMVADEQFGPVPAVVLESNIDDMNPELYEYVLERLFSVGA
                     QDVWTTPIVMKKGRPAVTLSILCGPAEEQAVREVLFSETTTLGLRRRAVEKWMLPREV
                     ITVQVEGGTVRVKVARAFGGRVTGASPEYADCVRVAREAQRPLKDVYAEAAAAARALL
                     TD"
     gene            complement(2187..4352)
                     /locus_tag="VM841_13900"
     CDS             complement(2187..4352)
                     /locus_tag="VM841_13900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVL91322.1"
                     /translation="MTLVVRNRTGSDVTPAFIERLARCLERDATEVIVADETIRMAST
                     DLLIAAEKAGLPVRVIRARAALADALVTAAGDYVAVMDADERHPPEMLSAMLFQAAAN
                     DVDIVMGSRRPVDGKPPRTLLSVCGDAVRGITDPATPFFVARRSMLEEFTPKPGATVL
                     ALDLLLAHPRASVSELPIKPARPGDAPGVRRRAAERVHIVRLSRRGAAVRAKAVRETA
                     NGLAWKARRKAEEPWTLFAIGGILIAAALLRFWALPSLHWYGHDEAHASMAAQRMLVD
                     HKPVLTGQPTSRGVYLGPGYYYLVAPFYALFGMSPFAGAFLAAIAGVGAVYLVWRIGR
                     ETFGALAGLSAAAIMATAPIAVYFGRFGWNPNTLPFFAALLIYALIRAPSNKRWLAPA
                     AFAAGLAPQLHATGLILPATLGIWLLTRRPKLRKRSWAGLAGLFALPLVPMAVAELRS
                     GFAGTRSWIRLLLGGGEPGSETGGSFLKAFKDLFVQTIGTPRFAVAASIVLAAIAGLI
                     FWERNHWSDRRVAPAFQTLMLFELLGFIGALLWRGEMFAYWLLPWLPGAIVLFAGGLV
                     EITYKTFAWVTKRTAALFVPATAVLLIVGLNIASVAAPVRLPDQLDATSPAAFQNVAA
                     AADWIAERADGPFRVEVWTPYVWDTARNSTRAFDYLLLRKGLKATPGAPTLFVISLED
                     IDFPKTRVVRNSVLAQMGEPVDRTAFGALGVFEYATASR"
     gene            complement(4570..>6353)
                     /locus_tag="VM841_13905"
     CDS             complement(4570..>6353)
                     /locus_tag="VM841_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HVL91323.1"
                     /translation="QTSFLDGPLAWNTHYRYRIIAKRELTINKPADPNTGVPADPNHV
                     ESGFSPSHPAGDGVGITTPPIPAPGDPTNLIATFSAPSTSNLQWIDNSTDEDGFLIEF
                     GPDGTNWLAQTAVGPHTSPATGPMTAMQVAIPPDTIRYYRVFAYRVTPYAKSNPSNVA
                     MIVAIPRAPTNLAETSVTSFQSVLQWKDNSNIETGFRIEYCNPSLFACTSPGTTGWAT
                     AGTTGPSSGTGALLSFVDGRFAASTLKYRVVAFNASGDSAPSDAITSTTPAGPLVTPL
                     NLTATPAGPHQINVAWSDTVDDKGYRLEFSDSGPSGPFAHLATTGQNVTTYADAPLPG
                     GKYRCYRVRAIDVAANRRSDPSNVACATTPMGPIPAAPTNAVAVPWSGNPTMAVEFTW
                     QDNSTNEDGFIVEHSSNNSTWREVGRTLPNVSVLRIGNYDPNSVQYFRVRAYNFEGLS
                     GYSNTASVLLLGPPRPVWLNPSKDNMVATSACYIDGTFAHTFSPAINDINIVIVRKLG
                     ADTGTYTCNTSPGSCTNQGGLVPISSEPAVNQVQLNWNGPGTFRLEFHFLKNVTYELH
                     ARAINVHGASASAFIASFVVLADCPLG"
BASE COUNT          965 a         2146 c         2282 g          960 t
ORIGIN      
        1 ggttggcgtc ctttgcgtcg gccgcgtccc caccctggag caccgccatg gaccgtttga
       61 gcgcatccac cttcgccgat gcccgaaacg cgagcgccaa agaagcgccg gcgactccga
      121 caccggccag cgcgacgaat cgcagcgcat caggatccca tggcatggct cgccctccag
      181 gcgaaaattc gcgcgaagta tacccttccc cctgtcgcgc tccggccgat agaatcgcag
      241 gcgtggaccg gccttcccat acgcagacgc tcgaccgact gctcgacgcg gtcgcgggcg
      301 gatcggtcac accctcggag gcggtggatc acatcgccgc cctgcgggct cgccggcgcc
      361 tcgacgacgc gcatgcgcgg ctcgaccttc accgccaggc gcggaccggc atgcccgagg
      421 tcatctattc gccgggcaag acggtcgtgc aaatgcggtc catcgcgcgc gtcctgctcg
      481 aacgctccga cgcaccggtg atcgccacga aagccacgcg cgagatgttc tcggcggttc
      541 gcgatctttc gccgaccgcc gcctacaacg agaccgcgcg caccatcgtg ctccgccgcg
      601 agcacggctc gccgatcggg cgctgcatgg tcgtgaccgc cggaacctcc gacatcccgg
      661 tcgccgagga agcggccgtc accgccgatg cgtgcggcgc gaaggtcgag cgcgtcttcg
      721 acgtcggcgt cgccggtatc catcgcatcc tcggggtctc cgacgacctg cgcaccgccg
      781 acgtcatcat cgttgcggcc ggcatggagg gggcgctgcc cagcgtggtc ggcgggctcg
      841 tccaggcgcc ggtcgtcgcg gtgccgacct cggtcggcta cggcgcctcg ttcggcggca
      901 tcgccgcgtt gctgggcatg ctcaactcgt gctcgcccgg cgtggcggtc gtcaacatcg
      961 acaacggttt cggagcggcc gcgttcgcgg tccggatgct tcgagcacgc gcgtgagcac
     1021 cgtcgcgtac ttcgactgct tcagcggcgt cgccggggac atgacgctcg gcgcgctgat
     1081 cgacgccggc gttccgctcg acgagatcga agccggcctg gcgaagctgc cgatcgagcc
     1141 gttcgagctc gaggtcaccc gcgtcgagcg gctcggcatc agcgccatcg gcgtgcgcgt
     1201 gcgcgccgcc gacactgggg tcctgcgcac gtacgccgcg gttcgtgcgc tgatcgagca
     1261 ggccgagctg gccgagcggc ccaaggagct ggcgctcacg atctttcgcc gcctcgccga
     1321 ggccgaggcg aaggtgcacg gcaaggaggt gtcgcgagtc gcgttccacg agctcggatc
     1381 cgtcgacacg ctcgtggaca tcgtcggcgc gtcgatcgcg ctggatctgc tcggcatcga
     1441 agaggttcat gcttcggcgg tcgcgaccgg catgggcatg atccgcaccg accacggcat
     1501 ctacccggtg ccggggccgg cggtcgtcga gctgctcaag ggcgcgccga tgtactcgcg
     1561 cggcatcccg accgagctcg tcaccccgac cggcgcggcg atcctggccg ccacggcgcg
     1621 cagcttcggc gacatgccgc agattcgcgt caagaccgtc ggctacggag ccggcacgcg
     1681 cgagctcgag atccccaacg tgttgcgggt catcgtcggt gagatggtcg ccgacgagca
     1741 gttcggtccc gtgccggccg tcgtgctcga gtccaacatc gacgacatga atcccgaact
     1801 ctacgagtac gtgctcgagc ggctcttctc cgtcggggcg caggacgtgt ggacgacccc
     1861 gatcgtgatg aagaagggcc ggcccgcggt cacgctgtcg atcctgtgcg gacccgccga
     1921 ggagcaggcg gtgcgcgagg tgctgttctc cgagacgacc acgctcggtc tgcggcgccg
     1981 cgcggtcgag aaatggatgc tgccgcgcga ggtcatcacg gtccaggtcg agggcgggac
     2041 ggtgcgcgtg aaggtcgcgc gcgccttcgg cggccgcgtc accggcgcct cccccgagta
     2101 tgcggactgc gttcgggtcg cgcgcgaggc gcagcgtccg ctcaaggatg tctacgcgga
     2161 agccgcggcg gcggcgcgcg cgttgctcac cgactagcgg tggcgtattc gaagacgccg
     2221 agcgcgccga acgcggtgcg gtccaccggc tcgcccatct gcgcgagcac cgagtttcgg
     2281 actactcgcg tcttcgggaa gtcgatgtcc tcgagcgaga tgacgaacag cgtgggcgcg
     2341 ccgggcgtcg ccttgaggcc cttgcggagc agcagatagt cgaaggcccg cgtcgagttg
     2401 cgggcggtgt cccatacgta cggcgtccag acctcgaccc ggaagggccc gtcggcgcgc
     2461 tcggcgatcc agtcggcggc ggccgcgacg ttctggaagg cggccggcga ggtcgcgtcg
     2521 agctgatcgg ggagccggac cggggcggcg accgaggcga tgttcaagcc cacgatcagc
     2581 agcacggccg tggccggcac gaacagcgcc gcggttcgct tcgtgaccca cgcgaacgtc
     2641 ttgtaggtga tctcgaccag gccgccggcg aagagcacga tcgcgccggg cagccacggc
     2701 agaagccagt atgcgaacat ctcgccgcgc cacagcagcg cgccgatgaa tccgaggagc
     2761 tcgaagagca tcagggtctg aaaggcgggt gccacgcgcc ggtcgctcca atggtttcgc
     2821 tcccagaaga tcagcccggc gatcgcggcg agcacgatcg aggcggcgac ggcgaagcgc
     2881 ggcgttccga tcgtctgcac gaacagatcc ttgaaggcct tcaggaacga gccgccggtc
     2941 tcgctgccgg gctcgccgcc gccgagcagc aggcggatcc acgagcgcgt ccccgcgaag
     3001 ccgcttcgaa gctcggcgac cgccatggga acgagcggca gcgcgaacag gcccgccagg
     3061 ccggcccacg agcgcttgcg caactttggc cgccgcgtca gcagccagat cccgagcgtc
     3121 gcgggcagga tcagaccggt cgcgtgcagc tgcggtgcca ggccagccgc gaacgcggcc
     3181 ggcgcgagcc agcgcttgtt cgacggcgcg cggatgagcg cgtagatcag aagcgcggcg
     3241 aagaacggca gtgtgttcgg gttccagccg aagcggccga agtacacggc gatcggcgcc
     3301 gtcgccatga tcgcggcagc gctcagtccc gcgagcgcgc cgaacgtctc gcggccgatt
     3361 cgccacacca gatagacggc tccgactcct gcgatcgcgg cgaggaacgc gcccgcgaac
     3421 ggactcatgc cgaatagcgc gtagaacgga gcgaccaggt agtagtagcc cgggccgagg
     3481 tagaccccgc gggaggtggg ctgacccgtc agcaccggct tgtggtccac cagcatgcgc
     3541 tgggccgcca tcgatgcgtg cgcctcgtcg tggccgtacc aatgcagcga gggcaacgcc
     3601 cagaatcgca gcagtgccgc agcgatcagg atgccgccga tcgcgaacag cgtccagggc
     3661 tcctcggcct tgcggcgcgc tttccacgcc agcccgttgg ccgtctcgcg caccgctttg
     3721 gcccggaccg ccgcgccgcg ccgcgacaga cgcacgatgt gcacgcgttc ggcggcgcgc
     3781 cggcgcacgc cgggcgcgtc gcccggccgg gccggtttga tcggaagctc gctcacgctc
     3841 gcgcgtggat gcgcgagcag aaggtccagc gccagcacgg tcgcacccgg cttgggcgtg
     3901 aattcttcga gcatcgaccg gcgggccacg aagaacggcg tcgcgggatc ggtgatgccg
     3961 cgaaccgcgt cgccgcaaac cgacagcagc gtgcgcggcg gcttgccgtc gacggggcgt
     4021 cgcgagccca tcacgatgtc cacgtcgttg gcggcggcct ggaacagcat cgcggaaagc
     4081 atctcgggag ggtggcgctc gtcggcatcc atgaccgcca cgtagtcgcc tgcggcggtc
     4141 acgagcgcgt cggccagcgc cgcccgcgcc cggatcacgc gaaccggcag accggccttc
     4201 tccgccgcga tgagcaggtc ggtcgaggcc atccggatgg tctcgtcggc gacgatcacc
     4261 tccgtcgcgt cccgctccag gcagcgagcg agccgctcga taaacgcggg cgtgacgtcg
     4321 cttccggtcc ggttgcggac gaccagcgtc aagcgggggc cggaagcaac ggtccggccc
     4381 cgcgacgtcc gttgacgcgg cgcggtgggc atgatggctc gtcaggacaa cgagccgccg
     4441 gcgcgcctcg tttcgccgcc ctgcccggcc cgtcctccgc tgggcgatat gtcgcgaggc
     4501 gaaacgggca ccgcacgcag gagcgcccgc gcagcgtatg cgcgggcgct cgtctccccc
     4561 ctcggaagat cagcccagcg gacagtcggc cagcaccacg aacgaagcga tgaaggcgga
     4621 cgccgacgcg ccgtgaacgt tgatggcgcg ggcgtgaagc tcgtaggtca cgttcttgag
     4681 gaagtggaac tcgagccgga aggtccccgg accgttccag ttgagctgga cctggttgac
     4741 ggcgggttcg gacgagatcg ggaccaagcc gccttggttg gtgcacgagc ccggcgaggt
     4801 gttgcaggtg taggtgccgg tgtcggcccc gagcttgcgc acgatcacga tgttgatgtc
     4861 gttgatcgcc gggctgaagg tgtgggcgaa cgtgccgtcg atgtagcagg cgctggtcgc
     4921 gaccatgttg tccttcgacg ggttcagcca caccggccgc ggcgggccca gcagcagcac
     4981 cgatgcggtg ttggagtagc cgctcaagcc ctcgaagttg taggcccgca cccggaagta
     5041 ctgaaccgag ttcggatcgt agttgccgat gcggagcacc gacacgttcg gcagggtgcg
     5101 gccgacctcg cgccacgtgc tgttgttgct cgagtgttcg acgatgaagc cgtcctcgtt
     5161 ggtcgagttg tcctgccagg tgaactcgac cgccatcgtc gggttgccgc tccagggcac
     5221 cgcgaccgca ttggtcggag ccgccgggat cggtcccatc ggcgttgtgg cgcaggccac
     5281 gttcgacggg tcgctacggc ggttggccgc gacgtcgatc gcgcggacgc gatagcagcg
     5341 atacttgccg cccggcagcg gtgcgtcggc gtaggtggtg acgttttgcc ccgtcgtcgc
     5401 gaggtgagcg aacggacccg acggaccgga gtcgctgaac tcgagccggt agcccttgtc
     5461 gtccacggtg tcgctccacg cgacgttgat ctggtgcggc ccggcaggcg tcgccgtcag
     5521 gttcaacggc gtcaccagcg ggccggcggg cgtggtcgag gtgatcgcgt ccgacggcgc
     5581 cgagtcgccc gaggcgttga acgccacgac gcgatacttg agcgtcgagg ccgcgaagcg
     5641 tccgtccacg aagctcagca gcgcgccggt gccgctgctc ggtccggtcg tgcctgccgt
     5701 cgcccacccg gtcgtgccgg ggctggtaca ggcgaacagc gacgggttgc agtactcgat
     5761 ccggaatccg gtctcgatgt tggagttgtc cttccactgc agcaccgact ggaacgacgt
     5821 gacggaggtt tccgccaggt tcgtcggtgc gcgcgggatc gcgacgatca tcgcgacgtt
     5881 cgacgggttc gacttcgcgt agggcgtcac gcggtaggcg aagacgcggt agtagcggat
     5941 cgtgtccggc gggatcgcga cctgcattgc cgtcatcggt cccgtcgccg gcgaggtgtg
     6001 cgggcccacc gcggtctgcg cgagccagtt cgtaccgtcg gggccgaact cgatcaagaa
     6061 cccgtcctcg tcggtcgagt tgtcgatcca ttgcaggttg ctggtcgagg gcgccgagaa
     6121 ggtcgcgatc aggttcgtcg gatcgccggg cgccgggatc ggcggggtgg tgatgcccac
     6181 gccgtcgccc gcgggatgcg agggcgagaa gccgctctcg acgtggttcg ggtcggccgg
     6241 cacgccggtg ttcggatcgg cgggcttgtt gatggtcaat tcgcgcttgg cgatgatccg
     6301 atagcggtag tgcgtgttcc acgcgagcgg accgtccagg aacgaggtct gtg
//
LOCUS       DATLFO010000346         5634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_98409, whole genome shotgun sequence.
ACCESSION   DATLFO010000346 DATLFO010000000
VERSION     DATLFO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98409"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /gene="serS"
                     /locus_tag="VM841_13910"
     CDS             <1..852
                     /gene="serS"
                     /locus_tag="VM841_13910"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVL91324.1"
                     /translation="LQIGETLDMIDVERGARTSGSRFAYIKGQLALLEFALVRYAMGT
                     LVDKGFTPVVPPVLVRREAMEGTGFLPTDEAQIYKLPEDDLYLVGTSEVPLAALHQDE
                     YLPVEALPLRYAGFSTCFRREAGTYGKDTRGIFRVHQFDKVEMFSFTTPEQSWEEHEF
                     LLSVEEGILKELQIPYRVMNIAAGDLGQPAAKKYDVEAWMPGQAAYREVTSCSNCTDY
                     QARRLNCRIKTESGPQFVHTLNGTAIAVGRMLIAIMENNQREDGSVHVPHVLVPYAGF
                     ELLGPTT"
     gene            877..962
                     /locus_tag="VM841_13915"
     tRNA            877..962
                     /locus_tag="VM841_13915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:911..913,aa:Ser,seq:tga)
     gene            989..1080
                     /locus_tag="VM841_13920"
     tRNA            989..1080
                     /locus_tag="VM841_13920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1025..1027,aa:Ser,seq:gct)
     gene            1223..1295
                     /locus_tag="VM841_13925"
     tRNA            1223..1295
                     /locus_tag="VM841_13925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1256..1258,aa:Arg,seq:acg)
     gene            1437..1940
                     /locus_tag="VM841_13930"
     CDS             1437..1940
                     /locus_tag="VM841_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91325.1"
                     /translation="MDRQPLERDRIAALYESHAAAALRFAFLLIGRREAAEDVVHEAF
                     LRVFGRFADRRSPDFFRPYLNRTIVNLVRSEQRRAGAERSKLPRLRTSGVHTDPNVDP
                     RPDVVRALATLPVRQRAAIVLRYYEDLSEAQVADIMRCSRPAVRSLVARGSESLRPLL
                     EGLRHGI"
     gene            1930..3210
                     /locus_tag="VM841_13935"
     CDS             1930..3210
                     /locus_tag="VM841_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91326.1"
                     /translation="MESDLKALLDEAATAAPAATRITAEFRSSITRRRARTGGVLILS
                     LGLVAAGSLAIVTRSPSRIVVRPAADCGGQWQVHRTPVERRLPGSTGVDLLYAVSAGG
                     SLWAIGNEHGIDSGIMGVTTRWNGTSLERIEIPHQGPRDVIKDAATDDSSIWIAGWTL
                     QQTATTTPAEGFLMRWSGTRWEQETPRDDGPLFPTAIAVRGSTVAVGGYRENGLAFVM
                     MRTGSGSWQHYPLPADMKGPFVADVALAPDGTLVVLTIGSDPASVLVLEDGRFRRIYS
                     GAPPGTTGIMALSVAATDDMWVSGIDRTRNHVSMEHWDGRTWTPVDLPDIDVVPSFAV
                     RAAGDVWAVGQTRVQRPGSQFPPREGEYTGLEAVKTTFLHWDGTSWSTADANFPSEQS
                     RLWSVDVDASGRGIAVGGGDVKPLQAERMIAIRC"
     gene            complement(3597..>5634)
                     /locus_tag="VM841_13940"
     CDS             complement(3597..>5634)
                     /locus_tag="VM841_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HVL91327.1"
                     /translation="PGTVTTVAGGGDGDGYPATTVSVHARDVAEDAVGAVWFTQDSRL
                     RRVSPAGVLSTVAGKAGPGDSGDGGPVAGARFQSLLALALDNVGNLFVADNDARKVRM
                     IRASDGVVTTVAGSGAKVSSGDGGPATQAGFDSIFDVAFEDGFLYIADYVGGVIRRVR
                     PDGIIERVAGTGVHDFSGDGGPAVQAELNHPGGVAVRDGVVFIADTWNDRVRRVDVDG
                     TITTIAGSTRPARALDGYGYGLYAQEGVPATLAHLVYPHGVAIASNGDVIIADTFNHR
                     IRRVDNAGFMWTVAGTPVSWSPHDDGMPARISSVAFTNGLAIGPSQRILYSESDWGRQ
                     AIRAIGGDGTVRTIAGNDDVGFGGDGGPATHAMLTDCAGIDVGTDGSIYIADQYGHRI
                     RKVDPSGSISTVTGGRAFVRTSRELWGPNDVAVDAQGNVFIAELYNHRISKLAPDGSL
                     SVVAGGREVDRQLWLGDYGTGFVGDGLPATLASLVFPMGVDVDDAGNLYIADTNNARI
                     RMVDTAGIITTIAGTGTYGYSGDNGPATSATIGRVEDVAYDPVTGSVYFADRYVNRVR
                     RIDASGIVTTVAGNGTSDDSGDGGLATNAGVAYPRGVVPDGRGGFFVATVTFVRHVDA
                     AGIIRTIGGGGSAEIDGVPALQTAFRDLDGLAIDPAGNLLVSATRVRRIEAPLD"
BASE COUNT          948 a         1900 c         1821 g          965 t
ORIGIN      
        1 cttcagatcg gcgagacgct cgacatgatc gacgtcgagc gcggcgctcg caccagcgga
       61 tcgcgtttcg cctacatcaa gggacagctc gcgctgctcg agttcgcact cgtgcgctac
      121 gcaatgggca cgctcgtgga caagggcttc accccggtcg tgccgccggt gctcgtgcgc
      181 cgcgaggcga tggaaggaac cgggttcctg cccaccgacg aggcgcagat ctacaagctt
      241 cccgaggacg acttgtatct ggtcggcacg tccgaggtgc cgctcgccgc gctgcatcag
      301 gacgagtacc tgcccgtcga ggcgctgccg ctccgctacg caggattttc gacctgcttc
      361 cgccgcgagg ccggcaccta cggcaaggac acgcgcggca tcttccgcgt ccaccagttc
      421 gacaaggtcg agatgttctc gttcaccacg cccgagcagt cgtgggagga gcacgagttc
      481 ctgctgtcgg tcgaggaagg gatcctgaaa gagctgcaga tcccttatcg ggtgatgaac
      541 atcgccgcgg gcgacctggg ccagccggcc gcgaagaagt acgacgtcga ggcgtggatg
      601 cccggccagg ccgcctaccg cgaggtcacc agctgctcga actgcaccga ctatcaagcc
      661 cggcggctca actgccgcat caagaccgaa tccggcccgc agttcgtgca cacgctcaac
      721 ggcaccgcga tcgccgtcgg ccgcatgctc atcgcgatca tggagaacaa ccagcgcgag
      781 gacggatcgg ttcacgtccc gcatgtgctc gtcccctacg ccgggttcga gctcttgggc
      841 ccgaccacct aaccggtcgc tgctaccctt gcctgcggag ggctagagct aactggcaag
      901 ccagcagtct tgaaaactgc cgccgggcaa ccggcttcgg ggttcgaatc cccggccctc
      961 cgccgctcgg gtagaatgcc cgctccaagg aggcgtcgcc tagtctggtc tatggcgcgg
     1021 gattgctaat cccgttgggt cgcaaggccc tcgagcgttc aaatcgctcc gcctccgcca
     1081 aaatcgatca cgcggagtcg cgcgtgatcg attccttttt gcaccttccc gactcaagcg
     1141 gcggcccaca cgagtcgccg gccgacgacc cgatgaagcg ctcccacggc ccgcgtgatg
     1201 gggtagaatc ggacctcgcg ttgcggccgt agctcaatgg atagagcatc tgactacgga
     1261 tcagaaggtt cggggttcga atccctgcgg ccgcgctgac cgatgccaat cccgcccttt
     1321 tgggccgggt acgacctcaa cgaacgccgg gcaatttcat cgccccgccg cgagtacggc
     1381 cgggccgcta ccgatccggg cccccatgcg tttatggagg gatgactgaa gccacggtgg
     1441 accgacaacc cctggaacgg gaccgcatcg ccgcgctgta cgaaagccac gcggcagcgg
     1501 cgctgcgatt cgcgtttctg ctgatcggac ggcgcgaggc cgctgaggac gtggttcacg
     1561 aggcgtttct gcgagtcttc ggccggttcg ccgaccgtcg ctcaccggac ttcttccggc
     1621 cctatctgaa tcggacgatc gtcaatctgg tccggtccga gcaacgccga gccggcgcgg
     1681 agcgatcaaa gttgccccgg ctgagaacgt ccggcgtcca taccgatccg aacgtcgatc
     1741 cgcgaccgga cgtggtgcgc gcgctggcca cgcttcccgt gcggcagcgg gccgccatcg
     1801 tccttcgcta ctacgaagac ctgtccgagg cccaagtcgc cgacatcatg cgctgcagcc
     1861 ggccggcggt tcgttcgctg gtcgcgcgcg gctcggagtc gctccgccca ctgctagaag
     1921 ggttgcgcca tggaatctga tctgaaggct ctgctggacg aggcggcgac ggcggcgccc
     1981 gccgcgacgc ggatcacagc cgagtttcgt tcgtcgatca cgcggcggcg cgcgcgaacg
     2041 ggtggtgtgc tgattctgtc gctgggcctg gtggcggccg gatcgctggc gatcgtgacc
     2101 cgctcgccgt ctcggatcgt ggtgcggccg gccgcggatt gcggcggcca atggcaggtc
     2161 caccggaccc cggtcgaacg gcggctgccg ggatcgaccg gcgttgatct gctctatgcc
     2221 gtatcggccg gagggtcgct gtgggcgatc ggaaacgagc acggcatcga cagcggcatc
     2281 atgggagtga cgacgcggtg gaacggcact tcgctggaac ggatcgagat tccgcaccaa
     2341 ggcccgcgcg acgtcatcaa ggacgccgca acggacgact cgagcatttg gatcgccggc
     2401 tggactcttc agcaaaccgc gaccacgacg ccagccgaag gattcctcat gcggtggtcg
     2461 ggaacgcggt gggagcaaga gactccacgc gacgacgggc cactctttcc gaccgcgatc
     2521 gccgtccgag gatccactgt cgccgtcggc ggatacaggg agaacgggct cgcgttcgtg
     2581 atgatgagaa ccggatccgg ttcgtggcag cactatccgc tgccggccga catgaagggt
     2641 cccttcgtgg ccgacgttgc gctcgctccc gacggaacgc tcgtcgtgct gaccatcgga
     2701 tccgatcccg cctcggtttt ggttctggaa gacggccgct tccggcgaat ctactcggga
     2761 gccccgccgg gaacgacggg aatcatggcg ctttccgtcg ccgcgaccga cgacatgtgg
     2821 gtgtcgggga tcgaccggac gcgcaatcat gtttcgatgg agcattggga cggacgcacg
     2881 tggactcccg tcgatctgcc ggacatcgat gtggtcccgt cctttgccgt ccgcgcggcc
     2941 ggtgacgtgt gggccgtcgg acagacccgc gttcagcgtc ccggatcgca gtttccgccg
     3001 cgcgagggcg agtacaccgg gctcgaggcc gtcaagacga cgttccttca ctgggacggg
     3061 acatcgtgga gcacggccga tgcgaacttc ccctccgagc agtcccggtt gtggtcggtc
     3121 gatgtcgatg catccggcag aggaatcgcg gtcggcggcg gagacgtgaa gcccttgcaa
     3181 gcggaacgga tgatcgcaat ccgctgctag agcggacccg tccgcgagcg cagacgccct
     3241 tcgggctctt cgggttcgag atgaaccagg cgcggggccg gcacactgtt gctgctccgc
     3301 acactatgcc atggacctta tgccactgtg tgccacctag gccccgggtc gaaggcctct
     3361 caacgtctcc cgggagaccg ccttccccaa gcccgtaacc cgatgcattc ggcaaatgac
     3421 aagcatgtaa ctgtcactcg cgactgtcat tcggcaatct gattgccgac atggagcgaa
     3481 gtcagcccgc cgggaatcac acgtactcag cgtatgccgg ggtgggatcc acgtgagccg
     3541 taccacctct ccgggcttgg ggatcgcgac ttgcgacgcg atccgcccgg agcgctttag
     3601 tcgagagggg cttcgatacg gcggacgcgg gttgcggaga cgagcaggtt gccggcgggg
     3661 tcgatggcga gcccgtccaa atcccgaaaa gcggtctgca gcgcggggac cccgtcgatc
     3721 tcggccgagc cgccgcctcc gatcgttcgg attatccctg ccgcatcgac gtgtcgcacg
     3781 aatgtgaccg tcgcgacgaa gaatccgccg cggccgtcgg gtactacgcc acgcggatac
     3841 gcgacgcccg cgttcgtcgc gagaccgccg tcacctgagt cgtcggacgt cccgttgcct
     3901 gcaaccgtcg tgacgatccc ggacgcgtcg atgcgtcgca cacggttcac gtaccgatct
     3961 gcgaagtaca ccgagcccgt caccggatcg taggcgacgt cttccactcg gcctatcgtc
     4021 gcgctcgtcg cgggaccgtt gtctcctgaa tagccatacg tgccggtgcc ggcgatcgtc
     4081 gtgatgattc ctgcggtgtc taccatgcgg atccgcgcgt tgttcgtatc ggcgatgtag
     4141 aggttgccgg catcgtcgac gtctacgccc atcggaaaca ccaggcttgc gagcgtcgcc
     4201 ggcaatccgt cgccgacgaa tcccgttccg tagtcgccca gccagagctg acggtcaacc
     4261 tctcggccac cggcgacgac gctgagtgag ccgtccggcg cgagcttcga gatgcgatgg
     4321 ttgtagagct cggcgatgaa cacgtttccc tgcgcgtcca ccgcgacgtc gttcggtccc
     4381 catagttcgc gcgacgtccg cacgaaagcc cggccgccgg tgacggtgct gatcgatccg
     4441 gaaggatcga ccttacgaat acggtgtccg tactgatcag cgatgtagat cgagccgtcg
     4501 gtgccgacgt cgatgcctgc gcagtccgta agcatcgcgt gtgtggcggg gccgccgtcg
     4561 cccccgaatc cgacatcgtc gttcccggcg atcgtccgta ctgtgccgtc accgccgatc
     4621 gctcgaatcg cctgacggcc ccagtcgctc tccgagtaca gaattcgctg tgatggcccg
     4681 atcgcaagac cgttcgtgaa cgcgaccgac gagatcctgg cgggcatccc gtcgtcgtgc
     4741 ggagaccacg acaccggtgt ccccgcgacc gtccacatga accccgcatt gtcaacgcga
     4801 cgaatccgat ggttgaaggt gtcggcaatg atcacgtcgc cgttgctcgc gatcgcaacg
     4861 ccgtgcggat agacgagatg tgcgagcgtg gcgggcacac cctcttgcgc gtacaggccg
     4921 tatccgtatc cgtcgagcgc ccgagcggga cgggtcgacc cggcgatcgt ggtgatcgtt
     4981 ccgtcgacat cgactctgcg gacgcgatcg ttccacgtat cggcgatgaa gacgaccccg
     5041 tcgcgcaccg cgacgccgcc cggatggttc agctcggcct gcacggccgg acctccatcg
     5101 ccggagaaat cgtggacgcc ggtccccgcg acgcgctcaa tgatcccgtc gggccggacg
     5161 cggcggatca ctccgcccac gtaatcggcg atgtagagga atccgtcctc gaacgcgacg
     5221 tcgaagatcg agtcgaatcc cgcctgcgtc gcaggtccgc cgtcgcccga cgaaaccttg
     5281 gcgccgctcc ccgccacggt cgtgacaacg ccatccgagg cccgaatcat tcgcacctta
     5341 cgcgcgtcgt tgtcggcgac gaaaaggttt ccgacgttat cgagcgccag ggcaagcagc
     5401 gattggaagc gcgccccggc aaccggtccg ccgtcaccgg aatcgcccgg tcctgccttg
     5461 cctgcgacgg tcgacaggac tcccgcgggg ctgacgcggc ggagcctcga gtcctgcgtg
     5521 aaccaaaccg cgcccaccgc atcttcggcc acgtctcgcg cgtgaacgct taccgttgtc
     5581 gccggatagc cgtctccgtc gccgcctccg gcgaccgtcg tgacggtacc ggga
//
LOCUS       DATLFO010000347         4646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_146554, whole genome shotgun sequence.
ACCESSION   DATLFO010000347 DATLFO010000000
VERSION     DATLFO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146554"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VM841_13945"
     CDS             <1..614
                     /locus_tag="VM841_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91328.1"
                     /translation="VVLSVLTILGGLRVWQNVWRAEALRGYVAALRTAQEPAVPHLTP
                     GTRTYLDTSARDFADLKTPFVAFRTLAEVWEQDFKAAKDRVSDVVPPAELLPAHADLI
                     QGLDMYAGVARLYHLASQQREIGAGLSGDARAAVEEKVQVTLQHAREWANRAAAVYSL
                     GGAAIEALELEWLGEEPSRALPEEPRFLVPTTPPPEVTPAPAG"
     gene            620..1510
                     /locus_tag="VM841_13950"
     CDS             620..1510
                     /locus_tag="VM841_13950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVL91329.1"
                     /translation="MGRSIALIANPASGRGRVGGFLPRVEQRLRDAGCTVETLISRSP
                     SHAIELAAGAGGRCDVVAAAGGDGMVHFVVNGLLGTPAALGIVPLGTGNDFAANLGYA
                     RRDPLAACAVLASGAERRVDAGKIEGGPSFVCVAGGGFDSEVNRVANRIRMLRGTPVY
                     VAATLRTLARFRPARFTVTLDGKASRMDAMFVAAGNATSYGGGMRITPDARLDDGLFD
                     VTVVGALSRPALLGQFPKLFKGTHVNHPAVTTARAATVTIAADRPFACYADGEEIGPL
                     PVTLTVQPGALRVVGPGVSA"
     gene            1507..4116
                     /locus_tag="VM841_13955"
     CDS             1507..4116
                     /locus_tag="VM841_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL91330.1"
                     /translation="MNEVEQFAAGYSFPLDPFQTTACESLARGRSVLVCAPTGAGKTV
                     VGEFAVALGIRRGRKTFYTTPLKALSNQKFGDFIARHGAANVGLLTGDNSINGEAPAV
                     VMTTEVLRNMLYEKSPTLHNLGFVVMDEVHYLQDAYRGAVWEEVLIHLPPSVQVVALS
                     ATVSNAEEFGDWLRHVRGKVDVVIEEARPVPLEHHYMVGSQVSPTFVGQGKNKRPNPQ
                     LKKMSLNAERRRGRDHMRARRAVPRREEVVDMLDDRSLLPAIYFIFSRNGCDQAVKQI
                     LTGSTRLTSSEESERIAAYLELRAEMLPDTDLDVLGYDDWRDGLMRGVASHHAGLIPL
                     FKETVEELFEQGLIKVVFATETLSLGINMPAKTVVLEKLIKFTGEKHELMTPGEYTQL
                     TGRAGRRGIDPVGHAVVLFQPEVAFDRVAALVGARTYPLRSSFRPSYNMAVNLARTYT
                     VAEALALIEKSFAQFLVQKQASGLHAQVERSEKFLEGYRERMTCDAGDFAGYWSMVRR
                     IRDEEKDAAGDGETHKEGERILSRLRIGDVVRIGGRGRGMLAVVAVRRGGTPMALTTS
                     DTMKRLGVRDVIGDRPVLGRIELPRRYPWKSKERGEVLAALKRFQVPSAPVEPVPPAP
                     AVEPDLPAGSVWAAIRAHPCHDCPDRTEHERWAARVDDLTKELTAQRRRLRNRTEGLA
                     RTFERVVGVLREKSYLRADEGLTHKGDRLSRIYNEGDLLVAEVLEQEILHGLEVPDLA
                     AAVSTLVYNARGFAVEPAWPNETVRQAYIKIRRLWREIEKTEERHGLALTREPEPGFV
                     DAIHAWASGESLDEILDFTEMSPGDFVRSTKQVWDLLRQLAEVADGEELRGRCRKAAD
                     HVYRGVIAYSGAL"
     gene            4222..>4646
                     /locus_tag="VM841_13960"
     CDS             4222..>4646
                     /locus_tag="VM841_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91331.1"
                     /translation="MRPGIRLLRTAVAGGLLFALMLMPARAAAVGPVIWHTPVPYAAA
                     GTSIGVTAQATCDTPCTARIYYRTTVANAPAELLTRDDPSWANLIMGRDPGIIIGQQT
                     LYSFFVQLPASIADSRGVDYIIKVTDGSAVSWSPGTPATA"
BASE COUNT          699 a         1617 c         1630 g          700 t
ORIGIN      
        1 tcgtggtgct gtcggtgctg acgatcctcg ggggcctgcg cgtgtggcag aacgtctggc
       61 gcgccgaggc gctgcgcggc tacgtcgcgg cgcttcgaac cgcgcaagag ccggcggtgc
      121 cgcacctgac gccgggcacg cgcacgtatc tggacaccag cgcccgcgac ttcgccgacc
      181 tcaagactcc gttcgtcgcg ttccggacgc tcgccgaggt atgggagcaa gacttcaagg
      241 ccgccaagga ccgcgtctcc gatgtcgtgc cgccggcgga gctcctcccg gcgcacgcgg
      301 atctgatcca agggctcgac atgtatgcgg gcgtcgcacg tctgtaccac ctcgcgagcc
      361 agcaacgcga gatcggagcc gggctcagcg gcgacgcgcg cgccgcggtc gaggagaagg
      421 tccaggtcac actgcagcac gcacgggagt gggcgaaccg cgccgcggcc gtctactcgc
      481 tcggcggcgc cgcgatcgag gcgctcgaac tcgagtggct cggcgaggag ccgtcgcggg
      541 cgctgcccga agagccgcgc ttcctggtgc ccaccacgcc gccacccgag gtcacgcccg
      601 cgccggcggg ctgatgcggg tgggccgctc gatcgcactc atcgccaacc cggcttcggg
      661 tcgcggacgt gtcggcgggt tcttgccgcg cgtcgagcag cggctccgcg acgcgggctg
      721 caccgtcgag acgttgatct cgcgttcgcc ctcccacgcg atcgagctgg cggccggagc
      781 cggcggccgc tgcgacgtcg tcgccgccgc gggcggggac ggcatggttc atttcgtcgt
      841 caacgggctg ctcggcacgc cggccgccct cgggatcgtg ccgctgggca cgggcaacga
      901 tttcgccgcc aacctcggct acgcgcgccg tgatccgctc gcggcctgcg cggtcctggc
      961 ctccggcgcc gagcggcggg tcgacgcggg caagatcgaa ggcggtccgt ccttcgtgtg
     1021 cgtggccggc ggcgggttcg actctgaggt caaccgggtc gccaaccgga tccggatgct
     1081 gcgcggaacc ccggtctacg tcgcggcgac gctgcggacg ctggcgcggt tccgcccggc
     1141 gcggttcacc gtgaccctgg acgggaaagc gagccgcatg gacgcaatgt tcgtcgcggc
     1201 cggcaacgcg acgtcgtacg gaggcgggat gcgcataacg ccggatgcgc ggctcgacga
     1261 cggtctgttc gacgtcaccg tcgtgggcgc gctctcgcgg ccggcgttgc tcggccagtt
     1321 cccgaagctg ttcaaaggca cgcacgtgaa ccatcccgcg gtgaccacgg cgcgcgcggc
     1381 gaccgtcacc atcgcggccg accgtccgtt tgcctgctac gccgacgggg aagagatcgg
     1441 accgctgccc gtgaccttga ccgtgcagcc cggcgcgctc cgcgtcgtcg gtcccggagt
     1501 gagcgcgtga acgaggtcga gcagttcgcg gccggatact cgttcccgct ggatccgttc
     1561 cagaccaccg cgtgcgagtc gctcgcccgg ggccgatccg tgctcgtgtg cgcgccgacc
     1621 ggcgcgggca agaccgtggt gggcgagttc gcggtcgctc tgggcatccg gcgcggacgc
     1681 aagaccttct acaccacgcc gctgaaggcg ctgtcgaacc agaagttcgg cgacttcatc
     1741 gcgcggcacg gcgccgccaa cgtcggcctg ctgaccggtg acaactcgat caacggcgag
     1801 gcgccggcgg tcgtgatgac gaccgaggtg ctgcgcaaca tgctctacga gaagtccccg
     1861 acgctgcaca atctcggctt cgtcgtgatg gacgaggtgc actacctgca ggacgcgtat
     1921 cgtggtgcgg tctgggagga ggtcctgatc caccttccgc cctcggtgca ggtggtcgcg
     1981 ctctccgcga cggtctccaa cgccgaggag ttcggcgact ggctgcggca cgtgcgcggc
     2041 aaggtggacg tggtcatcga agaggcgcgg ccggtgccgc tggagcatca ctacatggtc
     2101 ggcagccagg tctcgccgac cttcgtcgga cagggcaaga acaagcggcc gaacccgcag
     2161 ctcaaaaaga tgagtctcaa cgcggagcga cggcgcggcc gtgaccacat gcgagcccgc
     2221 cgcgccgtcc cgcggcggga agaggtcgtg gacatgctcg acgaccgaag tctgcttccg
     2281 gcgatttact tcatcttctc acgcaacggc tgcgaccagg cggtcaagca gattctgacg
     2341 ggctcgacgc gactgacgtc atccgaggag tccgagcgca tcgccgcgta tctggagctc
     2401 cgggccgaga tgctgcccga caccgatttg gacgtgctgg gatacgacga ctggcgcgac
     2461 gggctgatgc gcggtgtggc ctcgcatcat gccgggctca tcccgctgtt caaggaaacc
     2521 gtcgaagagt tgttcgagca gggcctgatc aaggtcgtgt tcgccaccga gacgctctcg
     2581 ctgggcatca acatgccggc caagaccgtc gtgctcgaaa agctcatcaa attcaccggc
     2641 gagaagcacg agctgatgac gccgggcgag tacacgcagc tgaccggccg cgcgggtcgt
     2701 cgcggcatcg atccggtcgg tcatgcggtc gtgttgttcc agcccgaggt cgccttcgat
     2761 cgcgtcgccg cgctcgtggg tgcgcgcacg tatccgctcc ggtcctcctt ccggccgtcc
     2821 tacaacatgg ccgtcaatct cgcccgcacc tacaccgtcg ccgaggccct cgcgctgatc
     2881 gaaaagtcgt tcgcgcagtt cctggtgcaa aagcaggcga gcggcttgca cgcgcaggtc
     2941 gagcgcagcg agaagttcct cgagggctat cgcgagcgca tgacctgcga cgcaggcgac
     3001 ttcgccgggt actggtcgat ggtgcgccgc atccgcgacg aggagaagga cgcggccggc
     3061 gacggtgaga cccacaagga aggggagcgt atcctgtcgc gactgcggat cggcgacgtc
     3121 gtcaggatcg gaggccgcgg acgcggcatg ctcgcggtcg tggccgttcg ccgcggcggc
     3181 acgccgatgg cgctgacgac ctcggacacc atgaagcggc tcggcgtgcg cgacgtgatc
     3241 ggcgaccgtc cggtcctcgg ccggatcgag ctgccgcgcc gctatccgtg gaagagcaag
     3301 gagcgcggcg aggtgctcgc ggcgctcaag cgcttccagg ttccgtccgc gccggtcgag
     3361 cccgtgccgc cggctcccgc cgtcgagccc gaccttcccg ccggcagcgt ctgggccgcg
     3421 atccgcgcgc atccttgcca cgactgcccc gaccggaccg agcacgaacg atgggccgca
     3481 cgggtggacg acctgaccaa ggagctgacc gcgcagcggc ggcggctccg caaccgaacc
     3541 gaggggctcg cgcgcacgtt cgagcgtgtc gtgggggtgc tgcgcgagaa gagctacctg
     3601 cgggccgatg agggcctgac gcacaagggc gaccggctca gccgcatcta caacgaaggc
     3661 gacctgctgg tggccgaggt gctcgaacag gagatcctgc acggtctgga ggtgcccgac
     3721 ctcgcggccg cggtgtccac gctcgtctac aacgcccgcg gcttcgcggt cgagccggcc
     3781 tggccgaacg aaaccgtccg ccaggcctac atcaagatcc ggcgcctgtg gcgcgagatc
     3841 gaaaagaccg aggagcggca cggcctcgcc ttgacgcgcg agccggagcc cggcttcgtg
     3901 gacgcgatcc acgcctgggc gagcggcgag tcgctcgacg agatcctgga cttcaccgag
     3961 atgtcgccgg gggacttcgt gcgctcgacc aagcaagtct gggatctgct gcgacagctt
     4021 gccgaggtgg ccgacggcga ggagcttcgg ggccgctgcc ggaaggcggc cgaccatgtc
     4081 tatcgcggcg tgatcgccta ctccggggcc ctctagccgg gcatttgccg cgattcggat
     4141 tctcccgcgg tcccgcttta ccgcctttcc cgtgatgcgc cgaagggatc cgcggggccg
     4201 ggcgcgaaat cttcttccaa gatgcgacca gggatccgtc ttctccgcac cgccgtggcc
     4261 ggcgggctgc tcttcgcgct catgctgatg cccgcgcgag ccgccgcggt cggtcccgtg
     4321 atctggcaca cgcccgtccc gtacgccgca gccggcacct cgatcggggt gaccgcgcag
     4381 gcgacgtgcg acacgccgtg caccgcgcgc atctactacc ggaccacggt cgccaacgcc
     4441 ccggccgagc tgctcacgcg cgacgacccg agctgggcga acctgatcat gggccgcgac
     4501 ccgggcatca tcatcggcca gcagacgctg tactcgttct tcgttcagct tcccgcctcg
     4561 atcgccgact cgcgcggggt ggactacatc atcaaggtca ccgacggctc cgccgtgtcc
     4621 tggtcgcccg gcacgccggc cacggc
//
LOCUS       DATLFO010000348        12781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18170, whole genome shotgun sequence.
ACCESSION   DATLFO010000348 DATLFO010000000
VERSION     DATLFO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18170"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..675
                     /locus_tag="VM841_13965"
     CDS             91..675
                     /locus_tag="VM841_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91332.1"
                     /translation="MRALLVAAVAAGALLAGADEARACSCRVRSPSAYLADAAAAFRG
                     AVVTLAHGSGGSAVALFEVDVVYKGEVPRAVTVRTGGEADTCAVPFRRDAVYVVFVTG
                     GPDARLAHLCGGTTDDRTYIEREGIEPVATYGPARPGAEPSANDESVAARPDAAARSR
                     AAPIALATIMALVAAMLLVRARNEAFSGPREPGA"
     gene            713..1741
                     /locus_tag="VM841_13970"
     CDS             713..1741
                     /locus_tag="VM841_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HVL91333.1"
                     /translation="MRIGILTGGGDAPGLNAVIRAIVRKATDVYGHAIIGFRNGWKGA
                     VDAEVETLTRESVTGILPRGGTILGSSRTNPFKIEDGVAKTKHTLQAFNIDALIPIGG
                     EDTLGVAAKLHEEGVKIVGVPKTIDNDLSGTDYTFGFNTAVEIATEALDRLHTTAEAH
                     QRVLVVELMGRHAGWIAAYAGIASGADAILVPEEAFDIEVVCEHLIRRHHRGRNFSIV
                     VVAEGAKPKEGTLVLQSEETDQFGHVRLGGIANMIAPEIEKRTGFETRVTILGHVQRG
                     GTPSAFDRVLATRFGVEAIDAVHEGDFGTMVAVQGNEIVRVPIGEGVGKLKTLPKSIM
                     DVAEVFFG"
     gene            complement(1738..2673)
                     /locus_tag="VM841_13975"
     CDS             complement(1738..2673)
                     /locus_tag="VM841_13975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL91334.1"
                     /translation="MPPNAVVYLLMLSVSLLWGLAFVGIKEALDHMSPATLTILRFAV
                     ADAAILLLALVWRGARPARFKGDEWRIGVLALLGIPVYHLAITWGEHRTSASVAALIV
                     GTAPVMVAVLSALVLRESVSTRRAAGIALAFAGVVVLAMKGSPAADGRPTQLSGVLVA
                     LLSPASWAVYTVVGRSILPRVKAMRLTATTFLVGGVMLLPFVRAETLREIAALPAEAW
                     AWTIFLGLGSSVAGYLIFNLALSMVEAAKIAVFIYMVPVVAVLSAALLLGEPLTAWVA
                     LAAAMVVGGVVITERDQRRARLESARRAGRESSAV"
     gene            2756..3787
                     /locus_tag="VM841_13980"
     CDS             2756..3787
                     /locus_tag="VM841_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HVL91335.1"
                     /translation="MDIVELRSDTFTTPTPEMRRAMAEAEVGDDVWGEDPTAIALQER
                     AAGIFGKEAGLFVPSGSMGNQMSIAAMTRPGDEVVCEAQSHIVLYEMGAPATIAQVML
                     RTIEAPDAILDPDAVAHTIRPASGHTTGTSLVSLENTHNARGGRIVPLDAMRAVAKVA
                     HERNVAVFLDGARIFNAAVATGIPVRDWAADVDALSFCFSKGLGAPVGSMIVGTQEHI
                     DRCRVLRKRYGGGMRQVGVLCAAARVALDTQIDRLADDHANARRLAEGWAEALPGCVD
                     PATVETNMVFADLQGRDAASVAAALWERGVRVGPTGPSAIRAVTHRDVTAEGIEAAIS
                     AFSEAVRAS"
     gene            3896..5305
                     /locus_tag="VM841_13985"
     CDS             3896..5305
                     /locus_tag="VM841_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91336.1"
                     /translation="MLLALAMFAPHASSASVPAASPVTGGDAGLCVRPVVETGASCTF
                     NVWGRVTDPDGPVINATVAHSGGRTRSDERGFFDLYVNSPGSYLVIVESRPGCRVPMR
                     VTVDAATAIRDGGVRRDVTLPCTRPTYNAFGFGVVQSGALVTIARSGVSHSGYAPGRI
                     DWFELRGGRVLAPQPFIDTAHDDRDAAGGLTRTGALVVFYAHFDQRANRWAGMRYVRA
                     AREGTVMGTIPHGSLVSFSPYGPIVILPSGRLLQTFYGTDAANVTRIYTSLSNDDGRT
                     WSPITPIEVSPPFRANEAAAVYLDGSSDARARLLMVARADGWSNGRRWFGLVQYSSSD
                     GGRSWRRHGLISITRSARESIPWLATLGGGRVAMVWADRGTLTLKRSVTRSSEALAMR
                     WAEGQVVYRSRVPDAPSPDIGDFGYPSIASYGPQDSRKAIVFNDIDPANSIGTMADVD
                     LYAIPLWASPADPPVTLSP"
     gene            complement(5289..6722)
                     /locus_tag="VM841_13990"
     CDS             complement(5289..6722)
                     /locus_tag="VM841_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL91337.1"
                     /translation="MSLRLRLTLVSTLVLAIVLTAFGTGVYLTLNKTLHDNLDQTLTR
                     YTDEIIRFYPNTEAFRSRVDYVAQIVSPGGTEQPIWPPSSVLLPSDERVLSVARGDEP
                     SYYADITVSGVPLRMRVTEVVDGSGRTVAALVVAAPRANIAETLNRLRLLLAWAGVIG
                     LALAAALSWQAAKTALRPVEEVADAATAIGETRDLSRRVDPGGEDELGKLTKAFNGML
                     DRLESAQTELERTLEGQRRFLADASHEMRTPLTTMRGNLEVIKASPAISPDELKAALT
                     DSIEEAERMSRLIADLLALARADARAQPREERVRLADVVREAVMGAQTADTASGGDGP
                     HVTVDADQSIVVRGSTEGLRRVVTNLVENAMKYTPGDGSVDVSVHREDQWAVLRVADT
                     GLGMTTEEVAHAFDRFWRSDRSRAERGSGLGLAIVKSVVEEHGGAIVAESEPARGTTM
                     WVRLPAEDDSITGALPPEEDSGVRATT"
     gene            complement(6728..7393)
                     /locus_tag="VM841_13995"
     CDS             complement(6728..7393)
                     /locus_tag="VM841_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL91338.1"
                     /translation="MGSRILVVDDDRKLRDMLRRALEGAGFEVETAEDGGRALAAVSA
                     HAPDLMVLDVLMPGVDGLGVARRLRERGDTTPILMLTARDSISDRVKGLDAGADDYLI
                     KPFSLEELLARVRAMLRRSHGEAEVLRYADVEIDPRTREVRRAGKPVELTAKEFDLLA
                     FFLREPRVVHERWRILEDVWGMESEVGSNVLEVYVGYLRKKLGDPNLIKTVRGIGYAL
                     RES"
     gene            7648..7944
                     /locus_tag="VM841_14000"
     CDS             7648..7944
                     /locus_tag="VM841_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91339.1"
                     /translation="MADKANRTGQSETARGNVLGDRAGWNKVAYNGAAHAPSDRTYGG
                     VNPRTMNYTHRADSGAFPGSLTNGALANLVPLVLSAMWESAFAREAGPLSTWSN"
     gene            complement(8116..9015)
                     /locus_tag="VM841_14005"
     CDS             complement(8116..9015)
                     /locus_tag="VM841_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVL91340.1"
                     /translation="MELALIGVTAVWGLTFPLVKCSVERCERVRGGFGLESAAPVDPF
                     LFLALRFAIAAIALAAIGARLPRSMIKPALWLGVALFAGYATQTLGLQRTSASNVAFI
                     TGLYVLMVPLMTAVVARRRPAATTVIGAIVATIGLFLMASPTGLGLGAGEILVLGTAA
                     SFAVHIFGTGLVAERVSPMDFVLVQSIVAAAASLAAAFIGGDAALPRDGSIWAAAILT
                     GVLASAVAFALQTRAQKRIPASRTAVILTAESVFGGLFGFLMLSERLGPRGYAGAVLI
                     TAGILIAEALVRDRGATASAAPQ"
     gene            complement(9060..10889)
                     /gene="pknB"
                     /locus_tag="VM841_14010"
     CDS             complement(9060..10889)
                     /gene="pknB"
                     /locus_tag="VM841_14010"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HVL91341.1"
                     /translation="MAQNIERERFPGYRLAGPLGGRYEITALIASGGMAEVFDARDSV
                     LDRRVAVKILHATYGNDAEFVERFRREAMAAGRLSHPNIVQVYDWGRNEDGASYMVME
                     RITGKTVREILIEKGALPVATAARLIEQVCRALDAARREGIVHRDIKPENILVTAEGQ
                     AKVADFGVARALAESRATQAGVVIGTASYLAPEQVEGKAGDHRSDIYSLGTVLYEMMT
                     GEAPFRGDNPVVVAYRRVAEDVPALRSIRRDAPAALEQVVARATARNPDDRFQTSAEM
                     AAALAPLAVGGEMEAPDVVAFDDTRTRAIPIAAAETTVIRRRTKPKRRRLIAALVFLL
                     AAGMVPIGVRVFGTVPVPDVTGMTQEAATSLIEEAGFNAEPVFRNDPAIEQGKVISVS
                     PEHGTKLRRSSRVTLAVSIGPLLIPVPDVRGKPLAEARTMLTDKGLEPLVSNRFTSSV
                     KKGIVFDQNPDPNVNVRSDAKVTIVVSAGPEQKVVPAVVGKPLTEATEMLKAEGFTVA
                     TKRVVDERVPRDQVMAASPNPGTKADKGTTVTLTVSDGPSEVPVPDVRGESYAEAVAR
                     LRAAGFTASRRQVPGSYGDTVVSQVPNAGRKAKRGSNVILHTA"
     gene            10970..11287
                     /locus_tag="VM841_14015"
     CDS             10970..11287
                     /locus_tag="VM841_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91342.1"
                     /translation="MADFVSDLETLGFRRTARRVPEWTHQASPYLIFSVQAEADGYIL
                     FTWELAIGEFMHAHGLQIGTNEALNLFLFPERDSRGPADVTFVVDEMDRVRKILSSLD
                     LSR"
     regulatory      11335..11447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11471..12244
                     /locus_tag="VM841_14020"
     CDS             11471..12244
                     /locus_tag="VM841_14020"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVL91343.1"
                     /translation="MEDRLVIAGRDFSSRLILGTGKFPSGKVMRDAIDASGTDMVTVA
                     LRRVDLDATGGPDILEFIDTSRILLLPNTSGAADAEEAVRLARLARAAGMPSWVKLEV
                     TPDPRYLLPDPVETLRAAEMLVADGFTVLPYMAADPALAKRLEEAGCATVMPLGSWIG
                     SSRGLRTADAIRIIVEQASVPVVVDAGLGAPSHAAEAMEMGVDAVLVNTAIAVARDPV
                     PMAAAFREATIAGRRAWTAGLSPVRDHAEASSPLTGFLT"
     gene            12241..>12781
                     /locus_tag="VM841_14025"
     CDS             12241..>12781
                     /locus_tag="VM841_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HVL91344.1"
                     /translation="MSFSEVLARTPVRDLAAESMRVTDADVTRALAKTDGTLDLHDFA
                     ALLSPAAATRIEEIARASHALTLRRFGRTIHMYAPLYLSNECLTTCTYCGFARTLPIA
                     RKTLSVAQTLAEARHLSARGFRHLLLLTGEHERLTGVDFLCEHLEALSPIVPHLSLEV
                     QVWSAEEYARLRDAGCDGVV"
BASE COUNT         1920 a         4444 c         4528 g         1889 t
ORIGIN      
        1 ccggccgccg ccgctgctgg gcgagcacac ccgcgagatc ctcggcgagc tcggctatgc
       61 gcgtgacgac gtcgagcgga tggccggatc gtgagggcgc tgctggtcgc cgcggtcgcc
      121 gccggcgcgc tgctggccgg cgccgacgag gcgcgcgcat gctcgtgccg ggtgcgctcg
      181 ccgtcggcgt acctggccga tgcggcagcc gcgttccgcg gagcggtcgt cacgcttgcg
      241 cacggatccg gcggctcggc cgtggcgctg ttcgaggtgg acgtcgtcta taagggcgag
      301 gtgccgcgtg ccgtcacggt tcgcaccgga ggcgaggccg acacgtgcgc ggttccgttc
      361 cgccgggatg ccgtctacgt ggtgttcgtc accggcgggc ccgacgcgcg gctcgcccat
      421 ctgtgcggcg gaacgaccga tgatcgcacc tatatcgagc gggaaggcat cgagcccgtc
      481 gcgacttacg gcccggcgag gccgggcgcc gaaccgagcg cgaacgacga gtcggtcgcg
      541 gcgcgtccgg acgcggccgc tcgctcgcgc gcagcgccga tcgcgctggc gacgatcatg
      601 gcgctggtcg ccgcgatgct gctcgttcgc gccagaaacg aggctttctc cggccctcgc
      661 gaaccgggcg cgtagtattg aaggatccgg cgaatcgtaa ggaggcccct cgatgcggat
      721 cgggatcctg accggcggcg gcgacgctcc cggtctcaac gcggtcatcc gcgcgatcgt
      781 gcggaaagcg accgacgtct acggccacgc gatcatcggc ttccgcaacg gatggaaggg
      841 cgctgtcgat gccgaggtcg agacgctcac gcgcgaaagc gtgaccggga tcctgccgcg
      901 cggcgggacg atcctgggat cgtcgcgaac caacccgttc aagatcgaag acggcgtcgc
      961 caagaccaag cacaccctgc aggccttcaa catcgacgcg ctgatcccga tcggcggcga
     1021 ggacacgctc ggcgtcgccg cgaagctcca cgaagaaggc gtcaagatcg tcggcgtgcc
     1081 caagacgatc gacaacgatc tgtcgggaac cgactacacc ttcgggttca acaccgccgt
     1141 cgagatcgcg accgaggcgc tggatcggct ccacacgacc gccgaggcgc atcagcgcgt
     1201 gctggtcgtc gagctgatgg gacgccacgc cggctggatc gccgcctacg ccggcattgc
     1261 ctcgggtgcg gacgcgatcc tggtccccga agaggccttc gacatcgaag tcgtctgcga
     1321 gcatctgatt cgccggcacc atcgcggccg caacttctcg atcgtcgtcg tcgccgaagg
     1381 tgccaagccg aaggaaggaa cgctggtcct gcagagcgag gagaccgacc agttcggtca
     1441 cgtgcgactg ggcggcatcg cgaatatgat cgcgcccgag atcgaaaagc gcaccggctt
     1501 cgagacgcgc gtgacgatcc tcgggcacgt gcagcgcggc ggcacgccca gcgccttcga
     1561 tcgcgtgctc gcgacgcgct tcggcgtgga ggcgatcgac gccgtgcacg aaggcgactt
     1621 cggcacgatg gtcgcggtgc aaggcaacga gatcgtgcgc gtcccgatcg gggagggtgt
     1681 cggcaagctc aagacgctcc cgaagtcgat catggacgtt gccgaagtct tcttcggcta
     1741 aaccgcgctc gactcgcgtc cggcgcgcct cgccgattcg agcctggcgc ggcgctggtc
     1801 gcgctcggtg atgaccacgc cgccgacgac catcgcggcg gccagcgcga cccaggcggt
     1861 gagcggctcg cccagcagca gcgcggccga aaggaccgcg accaccggca ccatgtagat
     1921 gaagaccgcg atcttggccg cctcgaccat cgacagggcg aggttgaaga tcaggtagcc
     1981 cgccacgctc gagccgaggc cgaggaagat cgtccaggcc cacgcttcgg cgggcagcgc
     2041 ggcgatctcg cgcagcgttt cggcgcgcac gaacggcagc agcatcaccc cgccgaccaa
     2101 gaacgtggtc gccgtcagcc gcatcgcctt tacgcgcggc aggatcgagc gtcccacgac
     2161 cgtgtagacc gcccacgagg cgggggagag cagcgcgacc agcacgccgg acagctgcgt
     2221 gggccgtccg tcggcggcag gcgagccttt catcgcaagg accacgacgc ccgcgaatgc
     2281 cagcgcgatc ccggcggcgc ggcgcgtgga gaccgattcg cgcagcacca gcgccgacag
     2341 caccgcgacc atcaccggcg cggttccgac gattagcgcc gcgacgctcg ccgaggtgcg
     2401 gtgctcgccc caggtgatcg ccaggtggta gacggggatg ccgagcagcg ccagcacgcc
     2461 gatgcgccac tcgtcgccct tgaagcgagc gggccgggcg ccgcgccaga ccaacgccag
     2521 caacaggatc gcggcgtcgg cgacggcgaa gcgcaggatc gtcagcgtgg ccggcgacat
     2581 gtgatcgagc gcttccttga ttcccacgaa cgccaggccc cacagcagcg agaccgacag
     2641 catcagcagg tagacgacgg cgttcggcgg catgggccga gtttagtccc ggcggcgcgc
     2701 ccgcgaccgg cgcatttcgg ccgccgccgt gcccgcgccg gtatcatcgg aggccatgga
     2761 catcgtcgag ctgcgttccg atacgttcac cacgccgacg cccgagatgc gccgcgccat
     2821 ggccgaggcc gaggtcggcg acgacgtctg gggcgaagac ccgaccgcga tcgcgctgca
     2881 agagcgtgcc gccgggatct tcggcaagga agcgggcctg ttcgtgccgt cgggctcgat
     2941 ggggaaccag atgtcgatcg ccgcgatgac gcggccgggc gacgaggtcg tctgcgaagc
     3001 gcagtcccac atcgtgctct acgagatggg cgcgcccgcc acgatcgcgc aggtgatgct
     3061 gcgaacgatc gaggcgcccg acgcgatcct ggatcccgat gcggtcgcgc acacgatccg
     3121 tccggcgtcg ggtcatacga ccggcacgtc gctggtcagc ctcgagaaca cgcataacgc
     3181 gcgcggcggg cggatcgtgc cgctggacgc gatgcgcgcg gtcgccaagg tcgcgcatga
     3241 acgcaacgtc gcggtgtttc tggacggggc gcgcatcttc aacgccgcgg tcgccacggg
     3301 catcccggtg cgggactggg ccgccgatgt ggacgcgctg tcgttctgct tctcgaaggg
     3361 gctgggcgcg ccggtcggct cgatgatcgt gggcacgcaa gagcacatcg accggtgccg
     3421 tgtgctgcgc aagcgctacg gcggcggcat gcgccaagtc ggcgtgctct gcgccgcggc
     3481 gcgcgtcgcg ctcgacacgc agatcgaccg cctcgccgac gatcacgcga acgcccggcg
     3541 gctcgccgaa ggatgggccg aggcgcttcc cgggtgcgtg gaccccgcga ccgtcgagac
     3601 gaacatggtc ttcgccgacc tgcagggccg cgacgccgcg tccgtcgctg ccgcgctttg
     3661 ggaacgcggc gtgcgggtcg ggccgaccgg cccgtcggcc atccgggccg tcacgcaccg
     3721 cgacgtcacc gccgagggta tcgaggccgc gatttccgct ttttccgagg ccgttcgggc
     3781 ctcctgatat cccggagacg tccggcctcg ccgctatcgc agcggggtcc gccgcgagga
     3841 ttttcccgat ccggggcgaa ccggtacctc tatgggccga gccaggatcc tcgccgtgct
     3901 gcttgcattg gcgatgttcg cgccgcacgc atcctccgcg tcggtaccgg cggcgtcacc
     3961 ggtgacgggc ggcgacgcgg gcctgtgcgt gcgcccggtc gtcgagaccg gcgcgtcctg
     4021 caccttcaac gtctggggcc gcgtcacgga cccggacggg ccggtgatca acgcgaccgt
     4081 cgcccattcc ggcggccgga cccgcagcga cgagcgcggc ttcttcgacc tgtatgtgaa
     4141 cagccccggc agctacctgg tgatcgtgga gtcgcgtccc ggctgccgcg tcccgatgcg
     4201 cgtgaccgtg gacgccgcga ccgcgatccg cgacggcggc gtgcggcgcg acgtgacgct
     4261 tccctgcacg cggccgacct acaacgcctt cgggttcggc gtggtgcaga gcggggcgct
     4321 ggtgacgatc gcgcgctccg gcgtgagcca tagcggctac gcgcccggcc gcatcgactg
     4381 gttcgagctc cgcggcggcc gcgtcctggc gccccagccg ttcatcgaca ccgcgcacga
     4441 cgaccgcgac gcggccggcg gcctcacccg gacgggcgcg ctcgtcgttt tctatgcgca
     4501 tttcgatcag cgtgccaacc gctgggcggg tatgcgatac gtgcgggcgg cgcgggaagg
     4561 caccgtgatg ggcacgatcc cgcacggcag cctggtgtcg ttctcgccgt acggcccgat
     4621 cgtcatcctg ccgtccgggc ggctgctgca gaccttctac ggaacggacg ccgcaaacgt
     4681 cacgcgcatc tacacgtcgc tgtcgaacga cgacggacgg acctggagcc cgatcacgcc
     4741 gatcgaggtg tcgccgccgt tccgcgcgaa cgaagccgcg gccgtctatc tggacggctc
     4801 gtcggatgcg cgggcgcgcc tgctgatggt ggcgcgcgcg gacggctggt cgaacggcag
     4861 gcggtggttc ggcctggtcc agtacagctc atccgacggc ggccggagct ggcggcgtca
     4921 cgggctgatc tcgatcaccc ggtcggcgcg cgagtcgatt ccgtggctcg cgacgctcgg
     4981 cggcggccgg gtcgcgatgg tgtgggccga tcgcggcacg ctcacgctca agcgcagcgt
     5041 gacgcgatcg tcggaggcgc tggcgatgcg ctgggcggag gggcaggtcg tgtaccggag
     5101 ccgcgtgccg gacgcaccgt cacccgacat cggcgacttc ggctatccgt cgatcgcctc
     5161 gtacgggccg caggactcgc gcaaggcgat cgtgttcaac gacatcgatc cggcaaactc
     5221 gatcggaacg atggccgacg tggatctgta tgcgatcccc ctgtgggcct cgccggcgga
     5281 cccgccggtc acgttgtcgc cctgacgccg gagtcttcct ccggcggcaa agccccggtg
     5341 atcgagtcgt cctcggccgg caggcgtacc cacatggtcg tgccgcgggc gggctcgctc
     5401 tcggccacga tcgctccgcc gtgctcctcg acgaccgact tcacgatcgc gagacccagg
     5461 ccgctgcctc gctcggcgcg agagcggtcg ctgcgccaga agcggtcgaa ggcgtgcgcg
     5521 acctcctcgg tggtcatgcc gaggccggtg tcggcgacgc gcaagacggc ccactgatcc
     5581 tcgcggtgca ccgatacgtc gaccgagccg tctcccggcg tgtacttcat cgcgttctcg
     5641 accagattgg tcactacgcg ccgcagcccc tcggtcgatc cgcgcaccac gatcgactgg
     5701 tcggcgtcca cggtcacgtg cggcccgtcc ccgcccgagg cggtgtcggc ggtctgtgcg
     5761 cccatgacgg cctcgcgcac cacgtctgcg agccgcaccc gctcctcgcg gggctgggcg
     5821 cgcgcgtcgg cgcgggccag ggcgagcagg tcggcgatca ggcggctcat gcgctcggct
     5881 tcttcgatcg agtcggtgag cgcggccttg agctcgtcgg gtgagatcgc gggtgacgcc
     5941 ttgatgacct cgaggttgcc gcgcatcgtc gtgagcggcg tacgcatctc gtgcgaggcg
     6001 tcggccagga agcgccgctg cccttcgagg gtgcgctcga gctcggtctg ggcgctttcg
     6061 agccggtcga gcattccgtt gaaggctttc gtcagcttgc cgagctcgtc ctcgccgccg
     6121 ggatcgacgc gtcgcgacag gtcgcgggtc tcgccgatcg cggtggccgc gtcggcgacc
     6181 tcctcgaccg gacgaagcgc cgtcttggcc gcctgccacg acagcgccgc ggccagggcg
     6241 agcccgatca cgcccgccca cgccagcagc agccgcagcc ggttgagcgt ctcggcgatg
     6301 ttggcgcgcg gcgccgccac caccagcgcg gcgacggtgc ggcccgagcc gtcgacgacc
     6361 tcggtgaccc gcatgcgcag cggcaccccc gacacggtga tgtcggcgta gtagctgggc
     6421 tcgtcgcccc gcgcgaccga cagcacgcgc tcgtccgagg ggagcagcac gctcgacggc
     6481 ggccagatcg gctgctcggt cccgccgggg ctcacgatct gcgcgacgta gtcgacgcgg
     6541 cttcggaatg cctcggtgtt gggatagaac cggatgatct cgtcggtgta gcgggtgagc
     6601 gtctgatcga gattgtcgtg gagagtcttg ttgagcgtca gatagacgcc ggtgccgaac
     6661 gcggtcagca cgatcgccag caccagcgtg gagacgagcg ttaagcgaag gcgaagcgac
     6721 atgccggtca ggactcgcgc agcgcgtagc cgatcccgcg caccgtcttg atcaggttcg
     6781 ggtccccgag cttcttgcga aggtatccga cgtagacctc gagcacgttc gagccgacct
     6841 cggactccat gccccagacg tcctcgagga tgcgccaccg ctcgtgcacc acgcgcggct
     6901 cccgcaggaa gaatgcgagc aggtcgaact ccttggcggt gagctcgacc ggcttgccgg
     6961 cgcggcggac ctcacgggtg cggggatcga tttcgacgtc ggcgtagcgc agcacctcgg
     7021 cctcgccgtg gctgcggcgc agcatcgccc ggacccgggc gagcagctcc tcgagcgaga
     7081 agggcttgat caggtagtcg tccgcgcccg cgtccaggcc cttgacccgg tccgagatcg
     7141 agtcgcgcgc cgtgagcatc aggatcggcg tggtgtcgcc tcgctcgcgc agccgccgcg
     7201 ccaccccgag gccgtccacg ccgggcatca gcacgtcgag caccatcagg tcgggcgcgt
     7261 gggcgctcac cgcggcaagc gcgcggccgc cgtcctcggc ggtctcgacc tcgaagccgg
     7321 ccccttccag ggcgcgccgc agcatgtccc gcagctttcg atcgtcgtcg acgacgagaa
     7381 tccttgatcc catggcgagc aaaatcgtat cgccgcgtcg ctgagagcgc cctgaggcaa
     7441 gcgaaccgtt ccacggccgt ccgttttgaa ggcatcacgc gtgacgccca cggcccgatt
     7501 cgagtgcggg ctagtccctt cgggctatgg gttcgcgctg ccgtttaggc cgggatagtc
     7561 gtgccgagac ggcaagaaga aaccttttga aaagtggttc gttgtttcag tcaaacgcag
     7621 cggggcgatg ccgaaagagg tagagagatg gcagacaagg cgaaccgtac cggacagagc
     7681 gaaaccgctc ggggcaacgt cctgggcgac cgtgcgggat ggaacaaggt cgcctacaac
     7741 ggcgccgcgc atgctccctc ggaccgcacc tacggcggcg tgaacccccg gaccatgaac
     7801 tacacgcacc gcgcggattc gggtgcgttc cccgggtcgc tgaccaacgg cgcgctcgcc
     7861 aacctcgtcc ccttggttct ttcggcgatg tgggagtcgg cgttcgctcg cgaggcgggc
     7921 ccgctctcca cctggagcaa ctgacatgat ggcaatcctg gaattcatgg cgcggcagat
     7981 cgcatttcgc cgcacgcgcc gctagcccgc agcacaagcg tatcgagcgc gactcctccc
     8041 ctctcccgcg cgcttctcct gaggcccttc cgttggtggc gacggaaggg cctcagtcat
     8101 gtgccggtca cgggatcact ggggtgcggc cgacgcggtt gcgccgcggt cgcgcaccaa
     8161 ggcctcggcg atcaggatgc ctgccgtgat cagcaccgcg ccggcgtagc cgcgcgggcc
     8221 gagccgctcc gagagcatca ggaacccgaa cagccccccg aagaccgact cggccgtcag
     8281 gatcaccgcc gtccgcgagg ccgggatgcg cttttgcgcc cgggtctgca gcgcgaacgc
     8341 gaccgcgctt gcgagcacgc cggtcaggat cgccgcggcc cagatcgaac cgtcgcgcgg
     8401 cagggcggcg tcgcccccga tgaaggcggc cgccagtgat gcggccgcgg cgacgatcga
     8461 ctgaaccagc acgaagtcca tcggggagac gcgctcggcg accaggcccg tgccgaagat
     8521 gtgcaccgcg aaggacgcgg ccgtgccgag caccaggatc tcgccggcac ccaggcccag
     8581 gccggtgggg gaggccatca gaaacagccc gatcgtggcg acgatcgcgc cgataacggt
     8641 cgtggcggcc ggccgccggc gcgccaccac cgcggtcatc agcgggacca tcagcacgta
     8701 cagcccggtg atgaaggcga cgttggaggc gctcgtgcgc tgcaggccga gcgtctgggt
     8761 cgcatagccc gcgaacagcg cgacgccgag ccacagagcg ggcttgatca tgctgcgggg
     8821 aaggcgcgca ccgatggcgg ccagcgcgat cgcggcgatc gcgaagcgaa gcgccagaaa
     8881 caagaaagga tccaccggcg cggccgactc caggccgaag ccgccgcgca cacgctcgca
     8941 tcgctcgacc gagcatttca ccaacgggaa ggtcaggccc cacaccgcgg tcacgccgat
     9001 caaggcgagc tccatcgcga agcggggagg gcgccgggcg ggggagcgag ccacgtcgct
     9061 caggccgtgt gcaggatcac gttcgagccg cgcttggcct tccggccggc gttgggcacc
     9121 tgcgacacga ccgtgtcgcc gtaggagccc ggcacctggc ggcgcgaggc ggtgaagccc
     9181 gcggcccgca gacgcgcgac cgcttcggcg taggactcgc cgcgcacgtc gggcaccggc
     9241 acctcgctcg gaccgtcgct gacggtgagc gtgaccgtgg ttcccttgtc ggccttggtg
     9301 ccgggattcg ggctggcggc catcacctga tcgcgcggca cgcgttcgtc gacgacgcgc
     9361 ttggtcgcca cggtgaaacc ctcggccttg agcatctcgg tcgcctcggt aaggggtttg
     9421 ccgaccacgg ccgggacgac tttttgctcg ggtccggccg agaccacgat cgtgaccttt
     9481 gcgtccgatc gaacgttcac gttcgggtcg ggattctggt cgaacacgat tcctttcttc
     9541 accgacgagg tgaagcggtt gctcacgagc ggctcgagcc ccttgtcggt gagcatcgtg
     9601 cgtgcctcgg cgagcggctt gcctcgaaca tcgggcaccg ggatcagcag cggcccgatc
     9661 gagaccgcga gcgtcacacg cgagctgcgc cgcagcttgg tgccgtgctc gggcgagacc
     9721 gagatcacct ttccttgctc gatcgccggg tcgttgcgaa agaccggctc ggcgttgaag
     9781 ccggcctcct cgatcaggga cgtggcggcc tcttgcgtca tgccggtcac gtccgggacg
     9841 ggcacggtcc cgaatacgcg gacgccgatc ggaaccatgc cggccgccag aaggaacacc
     9901 agcgccgcga tcagccgccg ccgcttcggc ttcgtgcggc ggcggatgac ggtggtctcg
     9961 gccgcggcga tcgggatcgc gcgggtgcgg gtgtcgtcga aggcgacgac gtcgggagct
    10021 tccatctcgc cgccgacggc gaggggcgcg agcgccgcgg ccatctcggc ggaggtctga
    10081 aaccggtcgt cggggttgcg ggcggtcgcg cgcgcgacga cctgctcgag cgcggccggc
    10141 gcgtcgcggc ggatcgaacg caacgccggc acgtcctcgg cgacgcggcg gtaggcgacc
    10201 acgaccgggt tgtcgccgcg gaacggcgcc tcgccggtca tcatctcgta gagcaccgtg
    10261 ccgagcgagt agatgtcgct gcggtgatcg ccggccttgc cctcgacctg ctcgggcgcg
    10321 aggtagctcg cggtgccgat cacgacgccc gcctgggtcg cgcgcgactc ggccagcgcg
    10381 cgggcgacgc cgaagtcggc gaccttggcc tggccctcgg cggtgaccaa gatgttctcg
    10441 ggcttgatgt cgcggtgcac gatgccttca cgccgcgccg cgtccagcgc gcggcagacc
    10501 tgctcgatca gccgcgcggc cgttgcgacc ggcaacgccc ctttctcgat gaggatctcg
    10561 cggacggttt tgccggtgat gcgctccatc accatgtacg aggcgccgtc ttcgttgcgg
    10621 ccccagtcgt agacctggac gatgttgggg tggctcagcc ggccggcggc catcgcctcg
    10681 cggcggaagc gctcgacgaa ctcggcatcg ttgccgtagg tggcgtgcag gatcttgacc
    10741 gcgacccggc ggtcgagcac cgaatcccgg gcatcgaaaa cttcggccat cccaccggat
    10801 gcgatgaggg ccgtgatctc gtaccgcccc ccgagggggc cggcgagccg gtacccgggg
    10861 aagcgttcgc gctcgatgtt ctgcgccacg tccggctaat tgtagggacc aagcccgctc
    10921 ccggcacgga ggatagggaa cgctcgctcg ggtatacagt gaaagaccga tggccgattt
    10981 cgtgtccgac ctcgaaacgc tcggtttccg ccgcaccgca cggcgggtgc ccgaatggac
    11041 gcaccaagcg agcccgtacc tgatcttttc ggtgcaagcc gaggccgacg ggtacatcct
    11101 gttcacctgg gagttggcga tcggcgagtt catgcacgcc cacggcctgc agatcggcac
    11161 caacgaggcc ctgaacctct tcctgttccc cgagcgcgac tcccggggac cggccgacgt
    11221 caccttcgtc gtcgacgaga tggaccgtgt ccgaaagatc ctttcttcgc tcgatctgag
    11281 ccgctagggg cggcccggcg gcgcgcggcc gctcggggct atactcgaag cgaaaatctg
    11341 atatcgggga gctcgggcga ccgggctgag aggctgcctg ccacgggcgg cgacccgcga
    11401 aacctgatcc ggataatgcc ggcggaggga ggatgacggc caccggacgc gattcggtgg
    11461 ccgcgctcat atggaagacc ggctcgtcat cgcgggacgc gacttctcgt cgcgtctgat
    11521 cctgggcacc ggaaagttcc cgtcgggcaa ggtcatgcgc gacgccatcg acgcaagcgg
    11581 caccgacatg gtcaccgtcg cgttgcggcg cgtggacctg gacgccaccg gcgggcccga
    11641 catcctcgag ttcatcgaca cgtcgcgaat cctgttgctg cccaacacct cgggcgccgc
    11701 cgacgccgag gaggccgtgc ggctcgcgcg cctcgcacgt gcggcgggca tgccgtcgtg
    11761 ggtcaagctt gaggtcacgc ccgacccgcg ctatctgctg ccggatccgg tcgagacgct
    11821 tcgcgcggcc gagatgctcg tcgccgacgg cttcaccgtg cttccgtaca tggccgccga
    11881 cccggcgctg gccaagcgtc tggaggaggc gggctgcgcg acggtgatgc cgctcggatc
    11941 ctggatcggg tcttcgcgcg ggctgcgcac tgccgacgcg attcgtatca tcgtcgagca
    12001 ggcgagcgtg ccggtcgtgg tcgacgcggg cctgggcgca ccgtcgcatg ccgccgaggc
    12061 gatggagatg ggcgtggacg cggtcctggt caacaccgcg atcgccgtcg cgcgcgaccc
    12121 ggtgcccatg gccgcggcgt tccgcgaggc gacgatcgcg ggtcgccggg cctggacggc
    12181 gggcctgtcg ccggtccgtg accacgccga ggcctcgagc ccgctcaccg gattcctgac
    12241 atgagctttt ccgaggtgct ggcgcgcacg ccggtgcgcg acctggcggc ggagtcgatg
    12301 cgcgtcaccg acgcggacgt cacccgcgcg ctggccaaaa ccgacggcac gctcgatctg
    12361 cacgacttcg ccgcgctgct ctcgcccgcc gccgcgacgc ggatcgagga gatcgcccga
    12421 gcctcgcacg cgctgacgct gcgccgcttc ggccgcacga tccacatgta cgcgccgctg
    12481 tacctgtcca acgagtgcct gacgacctgc acctattgcg gcttcgcacg gacgctgccg
    12541 atcgcacgca agacgctgtc ggtcgcacag acgctcgccg aggcgaggca tctgagcgcc
    12601 cgcggcttcc ggcatctgct gctgctcacc ggcgagcacg agcggctcac cggcgtggac
    12661 ttcttgtgcg agcatctcga ggcgctgtcg ccgatcgttc cgcatctgtc gctggaggta
    12721 caggtctggt cggcggagga gtacgcgcgg ctgcgcgacg ccggctgcga cggggtcgtc
    12781 g
//
LOCUS       DATLFO010000349        11135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18210, whole genome shotgun sequence.
ACCESSION   DATLFO010000349 DATLFO010000000
VERSION     DATLFO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18210"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VM841_14030"
     CDS             complement(<1..542)
                     /locus_tag="VM841_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91345.1"
                     /translation="MRSAAADITGEVGLVAALRAGDEQAFAALIDRYHSTMMRVASAY
                     VPSIAVAEEAVQEAWLGVLAGLDRFQERSSLRTWIFRILVNVARTKGKKERRTLPFSS
                     IEPEDDGDAASFLPLDDPSYPGHWVAPPVMPERAILGGEALAAIREAIEALPDAQRTV
                     IVLRDEQGWSSTDVCNVLAIS"
     gene            793..1326
                     /locus_tag="VM841_14035"
     CDS             793..1326
                     /locus_tag="VM841_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HVL91346.1"
                     /translation="MNAATPDTMRSPWATMHPAEIAAHEFPRTRKGYDPDAVRAWLRA
                     VAAAVDTLQGELAHVRAERDRLESVLRRAGEDPPASAVRTALMQARLRRRPGGYDRAE
                     VEALLEAAASEIARLETRSAVLEAEAARSRDAALREAALTDAVAQLQAEITSIRSRLP
                     SVTSPAPGSLNGIGPLL"
     gene            1323..2882
                     /locus_tag="VM841_14040"
     CDS             1323..2882
                     /locus_tag="VM841_14040"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVL91347.1"
                     /translation="MSTLPAVVCAGGSGPERVHATLASAAGDHALCVTSSIEEDGAAA
                     GLARVLRLEPWPGPHRALEQAVDEVAGEPVALLASGTTLRRNALRRAGELLGRGTAVV
                     VLAETRRKARALQRRIRLYPRATFQPGSGVVLSAEAAPVLRERGWLQPDATLTVADIE
                     AIAGPIRVLGAAIHPAPPRALEHMTVTVLIPAYNEEAWIGDTLRSLEQQTRRPDRVVV
                     VDDGSTDRTGVIAAALGARVLRPPRKQGQKATALNYGLAHIDTDAVIVLDADTLFHPE
                     AVEHLMVELERGTDATCGAVLPMNERGIWARGRAIEYSVAMRIHKRIQRGLGSVFVLS
                     GCISAFRTEALRTIGGFSERTITDDVDATWSLLTRRFSVSYTPEAISYTVEPPNWQIY
                     KAQMRRWAGALFQTLPYHVKRLHKKPSLALVVGAAMWDVVTVPIFFVSTAVMIATGHA
                     RASAMIGLWAAAAMTVTLVASISVIGVRRTLKSFPASLLMLWTNLYFFFEALVREWVL
                     RRRSLAWVKGH"
     gene            2954..3130
                     /locus_tag="VM841_14045"
     CDS             2954..3130
                     /locus_tag="VM841_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91348.1"
                     /translation="MGVRFAKGLAVTGIAMAKVAWIALAGIVALIAGVMTLGRGHRLV
                     AGRTLETGEGPRMS"
     gene            complement(3157..4458)
                     /locus_tag="VM841_14050"
     CDS             complement(3157..4458)
                     /locus_tag="VM841_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HVL91349.1"
                     /translation="MPIVLLAGMLPAPAAATGSRSVAAWLPYWDHDRGLDAIKSNGDQ
                     FGEVSPFWYRLAPSGAVEAYSGAGSATVRDAIKGAGALILPTISNEFDRDRVAAVIGD
                     SGRRAAHVQALVSLTTSQGYDGFDIDYEGLAAGDRANFTSFVGELGAAMRAAGKLLSV
                     TVHPKTSEPGTWDGPQAQDYVAIGAASDRVRIMAYDYHWATSEAGPIAPASWVDAVAA
                     FAAKTITPSKVQLGLPLYGYDWVGKQGNGVTYEQVQALIAKHQPTLQWSSTDQEPWFT
                     YRDAAGVTHTVWFADRRSTDMRLGSVTKHGLRGVVFWRLGREDAGVWTGTRNAFGSGT
                     APPAPAPADTTAPSVAITSPKPNAHLSLPSQTITYTASDNVGVARAELYVNGKLTYAD
                     TTGRGNFTVPTSSLPRGRHLFTVRVLDAAGNAAASNVYLKR"
     gene            4670..5938
                     /locus_tag="VM841_14055"
     CDS             4670..5938
                     /locus_tag="VM841_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVL91350.1"
                     /translation="MRPEKPTSPSLNAVTLDDLRRSERLMLGVRATAIPWALLQILTY
                     TTLPYPDGVKGAGLIIVAGLAAAVAMSRMLLPRARTHAAVLSLGITGLAADIMVVSGF
                     AWLFAFDQESALWAILLIIPLEGAIRFQLRGALWTWTAVTILYIAREVWGSDRYGYRL
                     QWDSVTFRMGIGLMIALVAGLMARDLVRQRAQLRRVNEELRRTDAIRSALVTTLAHDV
                     RNPLTAIRGTFSVLRRSELDPRLAELVNLTDRSAARLERLSLGLLDLARLEQGRLDLN
                     FRSVRVTDAVGSAVRFADPDGAVESVTDPTLTVRADPDRLEQVLVNLITNALRYGDPP
                     VTVSAEAAGEKVRIRVRDRGAGVSPAERDHLFEPFRPREAKGSVGFGLTIVKALTEAS
                     GGRVFYEDAEPGAAFVVELPAGGGVSASSS"
     gene            complement(5919..6542)
                     /locus_tag="VM841_14060"
     CDS             complement(5919..6542)
                     /locus_tag="VM841_14060"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVL91351.1"
                     /translation="MMALIEEIITWFGPVFQTAGYPIIATAVLLERSILLGLVVPGEV
                     TLALGAIYASRGDLEIVPVILIGFFAALVGESTGFWLGRRYGESLIKRLPYGDRMSEK
                     LSQVKEHFHDKGGWTVAIGRYATAAGAFVPFAAGAGRMPFRRFLAWDVPAIAVWAAGV
                     GVLGFVLGEQIDLIERILSRFGWIMLGLLVVGLGARFFYKGVKSSKR"
     gene            6722..7003
                     /locus_tag="VM841_14065"
     CDS             6722..7003
                     /locus_tag="VM841_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91352.1"
                     /translation="MTRRLRRTVSILWLSSLLLVVSPMTQAFAREMETDGMTGGGPAG
                     AASSRLVSDGLGWWWLLIVAGIVVGGALLVALFDTLRKRPPRIPRAHPA"
     gene            complement(7080..7568)
                     /locus_tag="VM841_14070"
     CDS             complement(7080..7568)
                     /locus_tag="VM841_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVL91353.1"
                     /translation="MDEADLILEANAEHARTFDASLLPKEPARKLAVVACMDSRLNVY
                     AALGLGTGDAHVVRNAGGLVTDDVVRSLMLSQWTLGTREIVIVQHTDCGLMRIREDET
                     RARIAAETGAEPPFALGAFQDLDVSVRESISRLTESPFIAHKSIRGFVYDVATGLLRE
                     VR"
     gene            complement(7618..8145)
                     /locus_tag="VM841_14075"
     CDS             complement(7618..8145)
                     /locus_tag="VM841_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVL91354.1"
                     /translation="MRRPIWIPALALALVAAACGTATTEPGADASPKPTAGGATHGDE
                     PMHGGSGTFAFGSPAGEAEADRTVEITAMDSLTFDPAAISVEKGEVVTFRVTNTGKID
                     HDFALGDEEFQKEHGQQMGGGGHHAEGTPAIGLKPGETKSLTWRFTTPGTVLYGCHVP
                     GHYEAGMVGTITVQG"
     gene            8440..9639
                     /locus_tag="VM841_14080"
     CDS             8440..9639
                     /locus_tag="VM841_14080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVL91355.1"
                     /translation="MREAVIVEAVRSPLGRRNGKLKDWHPVDLLGTVYKELIERTGID
                     PAQVDDVIAGCVSQVGEQALNVARNGWLAAGLPESVPGTTVDRQCGSSQQAAHFAAQA
                     VMSGACDIVIAGGVENMSRIPMGTSIQAGGFPFTQAMFERYDGNLVPQGISSELIAAK
                     WDLSREQVDEVGFRSQQRAQAATAEGRFQREIMPVKVTVDGNTELFDTDEGLRETTLD
                     KLGSLKPAFFDENVGTKFPQIKWVTTAGNSSQITDGAVAMMITTPEKAKELGVKPRAR
                     FHTMALAGDDPVLMLTAPIPATKKVLAKSGLKLDDIDLVEINEAFATVILAWRTELGV
                     SDEFYDQRVNVNGGAIALGHPLGASGARLMTTLLHELERTGGRYGLQTMCEGGGMANA
                     TIIERLD"
     gene            9655..9909
                     /locus_tag="VM841_14085"
     CDS             9655..9909
                     /locus_tag="VM841_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91356.1"
                     /translation="MFRIVVAVLAGLIIFGIGFSSIRGLTRPPGGGSGEPEPMDPRVR
                     VLYWCENCGGEIMVLAQGSGMPYRHCGEKMIRREEVLRDN"
     gene            complement(9916..10170)
                     /locus_tag="VM841_14090"
     CDS             complement(9916..10170)
                     /locus_tag="VM841_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVL91357.1"
                     /translation="MPVYDYKCSSCSERTEHILLEGEKPPKTCASCGGTLKRAWGGGR
                     LQVKFEGWGFSKTDGLLGDSHGPRKDFKQLRERAERIRDE"
     gene            complement(10232..10510)
                     /locus_tag="VM841_14095"
     CDS             complement(10232..10510)
                     /locus_tag="VM841_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HVL91358.1"
                     /translation="METETKDELANRLRRIEGQVRGIARMIQEDKYCIDVLTQVSAAV
                     AALDKVGLKVVGSHMRTCVRDAVAAPGGDAGEAHIQELVDSLERFLKA"
     gene            complement(10554..10787)
                     /locus_tag="VM841_14100"
     CDS             complement(10554..10787)
                     /locus_tag="VM841_14100"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HVL91359.1"
                     /translation="MPVSKSKRRRYQPPPKPKPKPSPMWVPVLVVVLLAIGVLDLIFY
                     YLGLPPFQQAPRWGLLAGFGFIATGFAVATQWR"
     gene            10907..>11135
                     /locus_tag="VM841_14105"
     CDS             10907..>11135
                     /locus_tag="VM841_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91360.1"
                     /translation="MSADDHDRTSERGERMKASAALLAGLIVLAFLLTQAAVQTKPGP
                     RLSRTDRLVSLIRAEDDRTTRLREEVEALRKE"
BASE COUNT         1816 a         3759 c         3754 g         1806 t
ORIGIN      
        1 gagatcgcca gaacgttaca gacgtccgtc gaggaccacc cttgctcgtc gcgcaagacg
       61 atgaccgtgc gctgcgcgtc cggcagcgcc tcgatggctt cgcggatggc cgccagcgcc
      121 tctccgccga ggatcgctcg ctccggcatc accggcggag cgacccagtg gcccggatag
      181 ctcggatcgt cgagaggaag aaacgatgcc gcatcaccat cgtcttccgg ttcgatcgag
      241 gagaaaggga gcgtgcgtcg ctccttcttc cccttggtcc gcgcgacgtt caccaggatg
      301 cgaaagatcc aggtccgcag gctcgaccgc tcttgaaacc ggtcgagtcc cgcaaggacg
      361 cccagccacg cctcttgcac cgcttcctct gcgacggcga tcgacggaac gtaggcggag
      421 gcgacgcgca tcatggtcga gtggtatcga tcgatgagcg cggcgaaagc ctgctcgtcg
      481 cccgcgcgaa gcgcagcgac gagcccaact tctccggtga tatcggcggc cgcggatctc
      541 attacgggct atcttcgcgg cgaccttgcc ggtgaagcaa agagggccac ggagacgcgc
      601 cggacattga tcaccatttc gacacgtccc gcacatccgc ggtgccggat tcgcccgccc
      661 gaccatgttt taacactccg tgcccgggaa cattcccctg cgagcgggcg accggatgcg
      721 cccccaccaa agagaaaccg ccaacacgtg taccccgtct gccctcagac gcacgaaaag
      781 gattggacac gcatgaacgc cgccaccccg gacacgatgc gatcgccttg ggcgacgatg
      841 catcccgccg agatcgcggc gcacgagttt cctcgcaccc gcaagggata cgacccggat
      901 gccgtgcgcg cctggcttcg cgcggtcgcc gccgccgtcg acacgctcca aggcgagctc
      961 gcacacgtgc gcgcggagcg cgaccggctc gagtccgtgc ttcggcgggc cggggaggat
     1021 ccgccggcat cggcggtgcg caccgccctg atgcaggcgc gcctgcgtcg tcggccgggt
     1081 ggctacgacc gcgccgaggt cgaagcgctc cttgaggcgg ccgcctccga gatcgcccgt
     1141 ttggagactc gctccgccgt gctagaagcc gaagcggctc gcagccgcga cgctgctctg
     1201 cgtgaagctg cgctcacgga tgccgtcgcc cagctccaag ccgagatcac atcgattcgg
     1261 tcgcggcttc cctcggtcac gtcgccggca cccgggtcgc tcaacggcat cggtccgctg
     1321 ctatgagcac gcttccggcc gtcgtctgcg ccggaggttc cggccccgaa cgcgtccacg
     1381 caacgctcgc atcggccgcc ggagaccacg cgctctgcgt cacgtcctcg atcgaggagg
     1441 acggagccgc cgccgggctg gcccgcgttc tccggctgga accgtggccg ggcccccatc
     1501 gggccttgga gcaggcggtc gacgaggtgg ccggcgagcc cgtcgccctc ctcgcgagcg
     1561 gcaccacact ccggaggaat gcgctccggc gcgccggcga attgctcggc cgcggcaccg
     1621 cggtcgtggt gctcgccgag acacgacgca aggcgcgagc gctgcaacgc cggatccgcc
     1681 tttacccgcg agcgaccttc cagccgggtt ccggtgtggt gctgtcggcc gaggccgcac
     1741 ccgtgcttcg cgagcgcggg tggctgcagc cggatgcgac gttgacggtc gcagacatcg
     1801 aggcgatcgc cggcccgatt cgcgtgctcg gtgccgccat tcaccccgcc ccgccgcgtg
     1861 ccctggagca catgaccgtt acggtcctga ttcccgccta caacgaggaa gcatggatcg
     1921 gggacacgct tcgctcgttg gaacagcaga cccgccggcc cgaccgcgtc gtcgtcgtcg
     1981 atgacggctc caccgatcgc acgggcgtca tcgcggccgc gctgggcgcc cgcgtgctgc
     2041 ggccgcccag gaagcaaggg cagaaagcga cggcgctgaa ctacgggctc gcccacatcg
     2101 acaccgacgc ggtgatcgtg ctcgacgcgg acacgctatt ccaccccgag gcggtcgagc
     2161 acctcatggt cgagctcgaa cgcggaacgg acgcgacatg cggcgcggtg ctgccgatga
     2221 acgagcgggg catctgggcg cgcggccgcg cgatcgagta ttcggtcgcg atgcgcatcc
     2281 acaagcggat ccaacgcggc ctcggctccg tctttgttct ttccggctgc atctcggcct
     2341 tccggaccga ggcgctccgc acgatcggcg ggttctccga gcggacgatc accgacgacg
     2401 tggacgcgac ctggtcgctg ctgacgcgcc gcttctcggt ctcctataca ccggaagcga
     2461 tctcctacac ggtggagcct ccgaactggc agatttataa ggcgcagatg cgccggtggg
     2521 caggcgcgct gttccaaacc ctgccgtacc acgtcaaacg cctgcataag aagccatcgc
     2581 tcgcgctggt cgtcggcgcg gccatgtggg acgtggtgac ggtgccgatc ttcttcgtga
     2641 gcacggccgt gatgatcgcg acgggccacg cccgcgcctc ggcgatgatc gggctttggg
     2701 cagcggcggc gatgacggtg acactggtcg cgtcgatctc ggtgatcgga gtgcgccgca
     2761 cgctcaaatc gttccccgcg tcgctgctga tgctgtggac caacctctac ttcttcttcg
     2821 aagcgctcgt tcgggaatgg gtgctgcgcc ggcgcagcct cgcatgggtc aagggacact
     2881 gaaagaggag gaacgaagca tgtacacggg agcaggatcg ttttcgggag caggcagcgg
     2941 tacgaccgca gtcatgggcg ttcgtttcgc gaaggggctc gccgtcaccg gaatcgcgat
     3001 ggccaaggtg gcctggatcg cgctggccgg gattgtggct ctgattgccg gcgtcatgac
     3061 gctcggccgc ggtcaccggc tcgtcgccgg caggactttg gagacgggcg agggcccgcg
     3121 aatgtcctag gcgcgggccc tcgcacgctc gtttcttcaa cgcttcagat agacgttcga
     3181 ggcggctgcg ttgccggccg catcaagcac gcgaaccgtg aacagatgcc tgcctcgagg
     3241 gagcgacgac gttggcacgg tgaagttgcc ccgcccggtc gtgtccgcgt aggtgagctt
     3301 accgttcacg tagagctccg cccgcgcgac tccgacgttg tcggacgcgg tgtaggtgat
     3361 cgtctgcgag ggaagcgaca gatgcgcgtt cggcttcggg gacgtgatgg caaccgacgg
     3421 ggccgtcgtg tccgccggag ccggcgccgg cggggcggtg ccgctcccga acgcattacg
     3481 cgtcccggtc cagacaccgg catcctcacg gccgagccgc cagaacacca cgccgcgcag
     3541 cccgtgcttg gtcaccgaac cgagtcgcat gtcggtcgat cgccggtcgg cgaaccagac
     3601 cgtgtgcgta accccggcag cgtcgcggta cgtgaaccac ggttcctggt ccgtcgagga
     3661 ccactggagc gttggctggt gcttcgcgat gagcgcttga acctgctcgt aggtcacgcc
     3721 gttgccctgc ttgcccaccc agtcgtagcc gtagagcggc aacccgagct ggaccttgga
     3781 cggcgtgatg gtcttggctg cgaacgcggc gacggcgtcg acccacgagg ccggggcgat
     3841 gggcccggct tcacttgtgg cccaatggta gtcgtaggcc atgatgcgca cgcggtcgga
     3901 ggccgcgccg atcgcgacat agtcctgcgc ctgagggccg tcccaggtgc ccggctccga
     3961 cgtcttgggg tgcaccgtca cgctcagcag ctttccggcg gcgcgcatgg cggcgccgag
     4021 ctcgccgacg aaggaagtga agttcgcgcg gtcacccgcg gcgagccctt cgtagtcgat
     4081 gtcgaatccg tcgtagccct ggctcgtggt caatgacacc aaggcttgca cgtgtgcggc
     4141 gcgccggccg gaatcgccga tgacggccgc gacacgatca cggtcgaact cgttgctgat
     4201 ggtgggcagg atcagcgcgc cggcaccctt gatagcgtcc ctgaccgtgg cggagcccgc
     4261 acccgagtac gcctcgaccg cccccgacgg cgcgaggcga taccagaacg gcgagacttc
     4321 cccgaactgg tcgccgttgg acttgatcgc atcgagcccg cgatcgtgat cccaataggg
     4381 gagccaggcc gcgactgagc gagaaccggt cgctgccgcc ggagccggga gcatgccggc
     4441 aagcaagacg atgggcatga cggcaagagt taggaacctt cgagtgacag tcataatccc
     4501 cccactcggc tgcagcccct tgcagccttg agcgtgacaa caaaatactt tagatcgact
     4561 ccagaaggtt ttaaacgccg aaacaagcaa ttgtttcggc aaaagcaagc ctttttccgc
     4621 ttcgatcccg aagtgcttgc tgtatccctg gtaaataggg catgtgaccg tgcgcccgga
     4681 gaagccgacg agcccatcgc tcaatgcggt gaccctcgat gatttgcgca gaagcgagcg
     4741 gttgatgctc ggcgtacggg ccacggcgat cccgtgggcg ttgttgcaga tactgaccta
     4801 cacgacactg ccctatccgg acggggtcaa gggtgcgggg ttgatcatcg tggcggggct
     4861 cgcggccgcg gtcgcgatga gcaggatgct gcttccgcgc gcacgcaccc atgccgcggt
     4921 gctatcgctg ggcatcacgg gcctggccgc ggacattatg gtggtctccg gcttcgcatg
     4981 gctgttcgcc ttcgatcagg agtcggcgct ctgggcgatc cttctcatca tcccgctcga
     5041 aggcgcgatc cgattccagc tccggggcgc gttgtggaca tggacggcgg tgacgatcct
     5101 gtacatcgcc cgcgaggtct ggggaagcga ccgctacggc taccgcctcc agtgggacag
     5161 cgtgacgttc cggatgggca tcgggctgat gatcgcgctc gtcgccggcc tgatggcgcg
     5221 agatctggtg cggcagcgcg cgcagctgcg ccgggtgaac gaggagctcc gccgcacgga
     5281 cgcgatccgc tcggcgctcg ttaccacgct cgcacacgac gtgcgcaacc cactgacggc
     5341 gatccgaggg acgttctcgg ttcttcgccg aagcgagctc gatccccgtc tcgcggagct
     5401 ggtgaacctg accgaccggt ccgccgcgcg actcgagcgg ctctccctcg gcctgctcga
     5461 cctggcgcgc ctcgagcaag ggcggctcga cctgaatttt cggtccgtgc gcgtcaccga
     5521 cgccgttggg tccgccgtgc gattcgccga tccggacggc gccgtcgagt cggtcaccga
     5581 ccccacgctc accgtccggg ccgatccgga ccggctcgag caggtgctgg tgaacttgat
     5641 caccaacgcc ctgcggtacg gggacccgcc ggtgaccgtc tccgccgaag cggccggaga
     5701 aaaggtgcgg attcgggttc gcgaccgcgg cgcgggagtg tctccggcgg agcgggatca
     5761 cctgttcgag ccgttccgtc cgcgggaagc gaaaggctcg gtcgggttcg ggctcacgat
     5821 cgtcaaggcg ctgaccgagg cgtctggcgg acgggtcttt tacgaggatg ccgagccggg
     5881 cgcagcgttc gtcgtcgagc tcccggccgg cggcggcgtt agcgcttcga gctcttgacg
     5941 cccttgtaga agaaccgggc tccgagcccc accacgagaa ggccaagcat gatccaaccg
     6001 aagcgcgaca ggatgcgttc gatcaggtcg atctgctcac cgaggacgaa gcccagcacg
     6061 cccacaccgg ccgcccacac cgcgatggcc ggaacgtccc aggccaggaa ccggcgaaag
     6121 ggcatgcggc ccgcaccggc cgcgaacggc acgaaggcgc ccgccgcggt ggcatatcgg
     6181 ccgatcgcaa ccgtccatcc ccccttgtcg tgaaagtgct ccttcacctg tgagagcttc
     6241 tcgctcatcc ggtcgccgta cggcagccgc ttgatcagcg actcgccgta gcgacgtccg
     6301 agccagaagc cggtggactc cccgaccagc gccgcgaaga atccgataag aatgacggga
     6361 acgatctcca agtctccgcg agacgcatag atcgcgccga gcgcgagcgt gacctctccc
     6421 ggcaccacga gccccagaag gatcgatcgc tcgagcagaa ccgccgtcgc gatgatcggg
     6481 tagcccgccg tctgaaacac agggccgaac caggtgatga tctcttcgat gagcgccatc
     6541 attcggtacc accttacccc gcatcgtggg atgctccctt cgcgatagca agccgcccgt
     6601 gtcgaatgaa tcatttcgac cacgcggcgt tccacctttg gttatcgact tccctcggcc
     6661 acgatgggat cctcgtcggg tcttcaccgg cggcggtgaa gcgtccacga gggaggcagc
     6721 gatgacgaga cgtctccgca gaacggtttc gatcctttgg ctttcgagtc tgctgctcgt
     6781 cgtttcgccg atgacgcagg cgttcgctcg ggagatggag accgacggca tgaccggagg
     6841 cggccctgcg ggcgccgctt cgtcgaggct cgtatcggac ggtctgggct ggtggtggct
     6901 gctcatcgtg gcgggaatcg tcgtgggcgg cgcattgctg gtcgcgctat tcgacaccct
     6961 tcgcaaacgc ccgccgagga ttccgcgcgc acatccggca taacggcaga gccggtcgcg
     7021 aggtgtgtcc gccggcgcaa ccgtgaagga gggccttcgg ggcctccttc aggctgcggc
     7081 taccgcacct cgcgcagcaa tccggtcgcg acgtcgtaga cgaagccgcg gatgcttttg
     7141 tgcgcgatga acgggctttc ggtgagccgc gagatcgact cgcgcacgct cacgtcgagg
     7201 tcctggaacg cgccgagcgc gaacggcggc tccgcgcccg tctcggccgc gatgcgcgca
     7261 cgcgtctcgt cctcgcggat gcgcatgagc ccgcagtcgg tgtgctggac gatgacgatc
     7321 tcgcgggtgc cgagcgtcca ttgcgacagc atcagtgagc ggaccacatc gtcggtgacc
     7381 agaccgcccg cgttgcgcac gacgtgcgcg tcgcccgtgc cgagaccgag cgcggcgtag
     7441 acgttgagcc gcgagtccat gcacgcgacg accgcgagct ttcgcgcggg ctccttcggg
     7501 agcagcgagg cgtcgaaagt tcgcgcgtgc tcagcgttcg cttcgaggat caagtccgct
     7561 tcatccatga cgcgcggcac tgtaccgcct ccggctgagg tgagcgcaag ccgcacgcta
     7621 gccctgaacg gtgatcgtcc cgaccatgcc cgcctcgtag tggcccggca catggcatcc
     7681 gtacagcacg gtcccgggcg tggtgaagcg ccacgtcagg ctcttggtct caccgggttt
     7741 gaggccgatt gcgggggtgc cctcagcgtg gtgtcccccg ccgcccatct gctggccgtg
     7801 ctccttctgg aattcctcgt ccccgagcgc gaagtcgtgg tcgatctttc cggtgttggt
     7861 cacccggaag gtgacgacct cacccttttc gacggagatc gcggcgggat cgaacgtgag
     7921 cgagtccatc gcggtgatct caacggtccg gtccgcctct gcctccccgg ccggggatcc
     7981 gaacgcgaac gttccgcttc cgccgtgcat gggctcatcg ccgtgcgtgg ctccgccggc
     8041 cgtcggtttc gggctcgcgt ccgcgcccgg ctccgtcgtc gccgttccgc acgccgcggc
     8101 aaccagcgcg agggccagcg cggggatcca tatgggtcgt cgcatcgcgg tctcctttcc
     8161 tccacccagt atcggtaccc gccccgcggc gcgcaggagt ccgccgtccc ttggtcgacg
     8221 gctcttcagc gaggaacgct cacgaacgta cccgaaccca ccgaggcgcg gcgagctacg
     8281 gatcgcgacg gcgcatccac caggccgccc tcgtcgctgc ggcgggcagc gccgcccccg
     8341 cgggcaggcg gaatagccgg ccccggtcgc tcggttgacc atatcgggag ggccgggcat
     8401 agttaccggt gggtagcttt gagacaggag aggattccca tgcgtgaggc agttatcgtc
     8461 gaagcggttc gttctccgct ggggcgacgc aacggcaagc tgaaggactg gcatcccgtc
     8521 gatctgctcg gcaccgtcta caaggaactg atcgagcgga ccggcatcga cccggcgcag
     8581 gtggacgacg tgatcgcggg ctgcgtgtcg caggtcggcg agcaggcgct caatgtcgcg
     8641 cgcaacggct ggctcgcggc gggactgccc gagtcggttc ccggcacgac cgtggaccgg
     8701 cagtgcggct cctcgcagca ggccgcgcac ttcgcggcgc aggccgtgat gtcgggcgcg
     8761 tgcgacatcg tgatcgcggg cggcgtggag aacatgtcgc gcatcccgat gggcacgagc
     8821 atccaggccg gcggctttcc gttcacgcag gcgatgttcg agcgctacga cggcaacctc
     8881 gtgccgcagg gaatctcgag cgagctgatc gcggcgaagt gggacctgtc gcgcgagcag
     8941 gtcgacgagg tcggcttccg ctcgcagcag cgcgcgcagg ccgcaaccgc cgaaggccgc
     9001 ttccagcgcg agatcatgcc ggtcaaggtc accgtcgacg gcaacaccga gctgttcgac
     9061 accgacgagg ggctgcgcga gaccacgctc gacaagctcg gctcgctcaa gccggcgttc
     9121 ttcgatgaga acgttggcac gaagttcccg cagatcaagt gggtcaccac ggcgggcaac
     9181 tcctcgcaga tcaccgacgg cgcggtcgcg atgatgatca ccacgcccga gaaggccaaa
     9241 gagctcggcg tgaagccgcg cgcgcgcttc cacacgatgg cgctcgccgg cgacgacccg
     9301 gtgctgatgc tcaccgcccc gatcccggcg acgaagaagg tgctggcgaa gtcgggcctc
     9361 aagctcgacg acatcgacct ggtcgagatc aacgaggcgt tcgcgacggt gatcctcgcg
     9421 tggcgcacgg aactcggtgt cagtgacgag ttctacgacc agcgcgtcaa cgtcaacggc
     9481 ggcgcgatcg cgctcgggca tccgctcggc gcgtcgggcg cgcgtctgat gacgacgctc
     9541 ctgcacgagc tcgagcgcac cggcggccgc tacggcctgc agacgatgtg cgaaggcggc
     9601 ggcatggcca acgcgaccat catcgagcgg ctcgactaac cgccgcgaga cgtcatgttt
     9661 cgcatcgtgg tcgcggttct cgcggggctg atcatcttcg ggatcggctt ctcgtcgatt
     9721 cgcgggctga cccggccgcc gggcggcgga tcgggcgagc cggaaccgat ggatccgcgc
     9781 gtgcgcgtgc tgtactggtg cgagaactgt ggcggcgaga tcatggtgct cgcgcaggga
     9841 tcgggcatgc cctaccggca ttgcggcgag aaaatgatcc gccgcgaaga ggtcctgcgc
     9901 gacaactaat gcgggctatt cgtcgcggat gcgctcggcc cgctcgcgaa gctgcttgaa
     9961 atccttgcgc ggcccatggc tatcgccgag caggccatcg gtcttggaga agccccagcc
    10021 ctcgaacttc acctgcagcc ggccgccacc ccacgcccgc ttgagcgtgc cgccgcacga
    10081 ggcgcacgtc ttgggcggct tctccccttc gagcaggatg tgctcggtcc gttcgctgca
    10141 cgaggagcac ttgtagtcgt agaccggcat cggctcttct ctcgtcgtcc ccaggtctat
    10201 ccacagctgg ggaaaagtca cacgggtgta attacgcctt gaggaatcgc tccagcgaat
    10261 cgaccagctc ttggatgtgg gcctcgccgg cgtccccgcc gggagcggcg accgcgtccc
    10321 ggacgcaggt ccgcatgtgc gagccgacga ccttgagccc aaccttgtcg agcgccgcga
    10381 ccgcagccga gacctgcgtg agcacgtcga tgcagtactt gtcttcctgg atcattcggg
    10441 cgatgcctct cacctggcct tcgatccggc gcaggcggtt ggcgagctcg tccttggtct
    10501 cggtctccat ggacggcatt ataccccaag ggggtatggc gtgcgcgcga ggcctagcgc
    10561 cactgggtcg cgacggcgaa gccggtcgcg ataaagccga agccggcgag caagccccaa
    10621 cggggcgcct gctggaacgg cggcaaaccc aggtagtaga agatcaagtc gagcacgccg
    10681 atggcgagca gcacgacgac caggaccgga acccacatcg gggacggctt cggcttgggc
    10741 ttcggcggcg gctggtagcg ccggcgcttc gacttcgaaa cgggcatgcg gggtctcgct
    10801 ccttcgaaag gttcttcaaa aaagttcggg ctttcgtccg aatcaacgac ccgcccgagg
    10861 cgtccgtaac gccaggggga cgaagacgcc cgataatacc aggaacatga gcgccgacga
    10921 tcacgatcgc accagcgagc gcggcgagcg gatgaaggcc tcggccgcgc tgctcgccgg
    10981 cttgatcgtg ctcgccttct tgctgaccca ggcggccgtg cagaccaagc ctggcccgcg
    11041 cctgtcgcgc accgaccggc tcgtcagcct gattcgcgcc gaggacgacc ggaccacgcg
    11101 gctgcgcgag gaggtcgagg cgctcaggaa ggagc
//
LOCUS       DATLFO010000350        19920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_137858, whole genome shotgun sequence.
ACCESSION   DATLFO010000350 DATLFO010000000
VERSION     DATLFO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19920
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137858"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..743
                     /locus_tag="VM841_14110"
     CDS             357..743
                     /locus_tag="VM841_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91361.1"
                     /translation="MISPWWLLLEGVYFAFCGALFFGAYMSKNEWMRASLASLALSII
                     AWRVLAVIPSWWLYYAEGQLKWGGQGCLQLDLACLKQAIKDTIVVIQNAVALGGFVVA
                     FWLYQKKFPKQLGAGEAKPEATGGYK"
     gene            740..1090
                     /locus_tag="VM841_14115"
     CDS             740..1090
                     /locus_tag="VM841_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVL91362.1"
                     /translation="MSATSNKPQIYDDYELKVVDPNWLAERVKPKRHIGLDADLCILC
                     RACEDVCPWDCIYMLSPEIVQDAETPALEGATKASSAIFVIDDNECTRCGICVDRCPS
                     DALFYVTTGAKKGR"
     gene            1116..1895
                     /gene="extP"
                     /locus_tag="VM841_14120"
     CDS             1116..1895
                     /gene="extP"
                     /locus_tag="VM841_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HVL91363.1"
                     /translation="MAEKPILQEQLDILKDKVQDSQVWKSIFRPGSWLRKGYKGTSRD
                     RALAMMNNILYHLHPVKLKRHGLKLTYTFCLGGLSFFLFVLLTITGIYLMFFYRPAAE
                     ITAQLAYQDMADLSAAVTFGQLTRNLHRWAAHLMVLSVFLHMARVFYHGAYKPPREFN
                     WVIGVNLLQITLLLSFTGYLLPWDQLAFWAVTVGTNMMGYTPVFGRQVKFVLVGGLEV
                     GPPTLIRWYVLHVLALPFVAVLLMAVHFWRIRKDGGISGPL"
     gene            1905..2750
                     /locus_tag="VM841_14125"
     CDS             1905..2750
                     /locus_tag="VM841_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HVL91364.1"
                     /translation="MSEEVGIDQETFDKAIAEGKPERVARALAKSAWVRKNRGPASAA
                     PAPAAPAPPGDGDGAEAPAAAAPAAPAAQAPAAAAPAAATATVPRLTPEERAARVAAA
                     TGTKVNGGGGRGTGAGETHRLLAFVPPDGIQRVEKRQEDKINVWPHLLAEEFLSLVIV
                     LAGLILFSSFVNAPSRELANPFLTPNPSKAPWYFLGLQELLRYFHPMVAGVTLPGLGL
                     FGLMAVPYVDKNPSMKPERRKLAIMLFTVFVLFWSVLTMIGVFFRGPGFNWIWPWQTG
                     LFVDL"
     gene            2756..3466
                     /locus_tag="VM841_14130"
     CDS             2756..3466
                     /locus_tag="VM841_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVL91365.1"
                     /translation="MMIAKISTTNLILIGIAVLVGLAGVGMITANIFAIMRAKKDGPA
                     SQRPLIGAPKQLSRRSFFGRTLFIGFGTSMTAFGTLSVGFLWPNLKGGFGAKITLPQT
                     AEEILKQIQETNEPYFNSNGRFYLVPYSQSSEGGNRYAQEGTAAGGLMALYQKCVHLG
                     CRVPFCANSQWFECPCHGSKYNRAGEWKLGPAARGLDRMVMSVEGGIVTVDTGNIITG
                     PPRGTNTTGQEKEGPFCV"
     gene            3492..4436
                     /locus_tag="VM841_14135"
     CDS             3492..4436
                     /locus_tag="VM841_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVL91366.1"
                     /translation="MNIVIFAAKDPRLVAGILVASALALIAIFLIASAMSSRRRKSEI
                     PWAIRPGPSDDELESKTLPRFLAVGSIFLLFLSVWLPGLWLREPTRIDLKEEQFLRES
                     IHRGEILYAEHAPEGTRGAHFSANCARCHGPEGEGALQPFKEKFDYAEPPLKYIVSRY
                     KAAGNNDEEIRQIIVDAIERGRPGTLMPTWGQSFGGPLNQQQIDDVVNFIYAIQEPIP
                     GAGDVPVTADSGAQLFDANCAVCHTHKDPETGEIVWGGVGPDLRGVVAQLGVQGVRDT
                     IHDGRINYNRPSMPTWAFLGDSAIDALVAFLQSLQTAR"
     gene            4442..5350
                     /locus_tag="VM841_14140"
     CDS             4442..5350
                     /locus_tag="VM841_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91367.1"
                     /translation="MESLLQFAPFALEGQWFGAAAVALVIVGIIIFIGTVYILLVAIY
                     GWLQAYLVTMVSLMIFSIILAAVWLFGVPGTPPGTGPRGTEPYWVVFLPDSEQGLEFK
                     EEIETFPADRSVAVPEAGPVGDTEWRIALPDEQYPGAINVDGEIDTIKVVLQPALAGY
                     FQQQGTGSGKPSDYSFRLEGRAPSLEEEALPVARLLFYPNGDVAKVDPGTQAVLETDR
                     IGDPASRLLAGIDIPATENHPAIRVFAYRNKGQVFMASAQWMVVSVILFVVHLLWLAS
                     YENKQKRRDAELAAGTTPPREPVTTG"
     gene            complement(5412..6323)
                     /gene="hemC"
                     /locus_tag="VM841_14145"
     CDS             complement(5412..6323)
                     /gene="hemC"
                     /locus_tag="VM841_14145"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVL91368.1"
                     /translation="MPRSVLRIGTRASAVALAQAESVRRAVQQIITRRNFELVPVTND
                     ADEAGEPGGRGQVIAEIRKGIADNKIDLAVHNASDLPVTGPAQVALSAITERIDPREA
                     LVTRRGSGLNSLKPGTRVGVTSALRAAQVLAMRRGLTPVRIGGALDIRLRALTAGEIE
                     ALVLPIAGLKRLGRESRAVEILPIDMVMPPAGQGFLAVECRINDKDMRAALGQVQHEL
                     SRRAFDAERTFFLEMGGDHEAPVAAIAKLDGPMVKLRGIVCSPDGGRIIREIEIGDDP
                     VKVGQSLADRMMHAGAAELLAMEPLDS"
     gene            complement(6575..7270)
                     /locus_tag="VM841_14150"
                     /pseudo
     CDS             complement(6575..7270)
                     /locus_tag="VM841_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     gene            complement(7389..8303)
                     /locus_tag="VM841_14155"
     CDS             complement(7389..8303)
                     /locus_tag="VM841_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91369.1"
                     /translation="MIVFPLIAALISAVFSFALFRQYRSRKRLSQLAWGVALAQYAIA
                     SVAVAAGIGGGWDPTLYRVYWVFGAMLNVPWLALGSIALLNKRAITLASLLMVVVGTI
                     WGFVAVWSSSVEAQALRTEQIPKGSEVWCPDPGWRKIIEPCEPARGLARTYSLPSFAI
                     VVGIAAVSGRKRDGVRPPRDRIRGNWIIAAGVTLNAFAGFALIGKGRGGPFSVVLALS
                     VTVMFVGFVLASRTPRNVVLYVTPDCHLCEEPRRVLRAMRIPFQEVDAAGDPDRFIRT
                     PILEVDGSERAEGPMDAAAVRRALRKRA"
     gene            complement(8304..9821)
                     /locus_tag="VM841_14160"
     CDS             complement(8304..9821)
                     /locus_tag="VM841_14160"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVL91370.1"
                     /translation="MNASDFVRATAQRLPDKPAVLFQDLSITYRELDEEIDAVAAGLA
                     SLGVRPGDRVAVLVHNVPPFITAYYGVMRAGAVMVPINVMFTGEEIAHILTDSEARVV
                     IAAEPFAPLVDGVRHTAQMLEHLVVTGPRAPMGALTWGQFTTRSATPPKVDTKPGDLA
                     VLGYTSGTTGKPKGAMLTHGNLLANLDQMSRVPLLQEAETDVVLLVLPLFHIYALNVI
                     LGLTLKVGATAVLMERFDSVGSLEAIEKHKVTILFGAPPMFIAWVNTPGVERADLSSV
                     RVAVSGAAALPAQVFEDFKRKTGVTIWEGYGLTETAPGVTSNAMGDVAKAGSIGKALP
                     GVEVRIIDEDGEDVEEGDPGEIAVRGTNVFGGYWRQPEESKGVFTEDGWFRTGDIGYA
                     DQDGYIFLIDRKKDLVIVSGFNVYPREIENVLYRHPKVAECAAIGIPHPYTGEAVKAL
                     VVLKEGELASEEELLDFCRRSLARFKLPEVIEFVRELPHLPTGKVAKRTLRARES"
     gene            complement(9864..11165)
                     /locus_tag="VM841_14165"
     CDS             complement(9864..11165)
                     /locus_tag="VM841_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91371.1"
                     /translation="MDARTVRLDVRTAPIVLDCDVVVCGGGSAGLAAAIAAARAGARV
                     ALIERYGFFGGNAVAAYVGTICGLYVRDGDEHRDLYDGFPREWAERLKTTGSAFGPVP
                     YKETAVLLYVPWAYKRLADEMVRAEPNITPLLHATVTEVVRSGRIIDAVVVGSKRGPL
                     AITAAVFVDASGDADLAFHAGCATETGGPGQRQFPSMQFLMENVDVQAAYAAGLDKLN
                     ELLSTIGQTPEWNLSRSGGAVLPTFRHGEAIGAMTRVSVEGRSPDMTDPFEAAVAEMN
                     GRDEAEKAARFLIAHMPGFANAFIADTPPQLGIRETRRGVGDYVLTGEDVLSAAPFDD
                     AVAAGAWPQEFHVAGKGTEYVWLEPGASYQVPYRALLARDADNLLLAGRCISATHEAL
                     ASSRVIAPSMAQGEAAGRAAALAARNGTALRNLDARDIANA"
     regulatory      11295..11380
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11491..12165
                     /locus_tag="VM841_14170"
     CDS             11491..12165
                     /locus_tag="VM841_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVL91372.1"
                     /translation="MKRSIAVFVAVCMTAGVLFVTASPAAALSSYENDFASRHNSIRA
                     SHGRKALAVYGDLVDIARRHSGRMASEGRIWHNPNLTSEVKNWQVVGENVGMGPSVPD
                     LMDAFMNSPGHRANILDGEYNQFGVGVVVKDGTIYVTVVFALRSSSSGSTTAKKPSST
                     TTSKSSTISKPRSTATSPRSTTTKPASKPAAAASAPVAPAPVAVPVAPPVRTVDMLVR
                     MIRMDS"
     gene            complement(12293..14401)
                     /locus_tag="VM841_14175"
     CDS             complement(12293..14401)
                     /locus_tag="VM841_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVL91373.1"
                     /translation="MDERRPKASTTSTALAEGALAPRDIPPPTRRPEPHGVRRRLPVR
                     VLRRLGRMRKWKLVLLTGLTIFAVGSSVIYFGPASLSAAPPLQNATLLDIDGRPLAEL
                     RPEQNRVVVGIDEIPEVVQEAFVAVEDERFWSHWGVDPIAIARALFANAQGDREGAST
                     ITQQYVKNTFVGNERTIWRKIREAITAIRVDRKFSKEKILAAYLNSIYLGNGAYGVMA
                     ASRLYFGHDINQATLEEAALLAGITASPGRFDPRKNPDGAKVRRNYVLERMRILGMIT
                     PEEETFAAAMPIDIAPPVKQVAIAPSFVDWARKVLEGEFGEDVLYRGGLRVTTSLDLD
                     VQRAAEEAIAELLDEPGDPEAAVVVIDVETGGVRAMVGGRGKRLGDLNLAVQGLRQAG
                     SAFKPFVLAAGLKQGKTLDDRYSAPGSMRLRLDNGTVWKVGNYDRRGYGRPTLKRALA
                     YSVNTVFAQLIRDVGASNVAKLAKATGVSASLRAVESLALGTSEVTAMDLATGYATFA
                     REGTYLRTTALTEVKNREGDMLYRNRKAGDRSGRRAVTFDVASDVLDAMKEVVRIGTG
                     RKARIPDYVTWGKTGTTEDFADAWFVGGAGSAVAAVWVGHPQGRVPMRGVHGINVVGS
                     SFPLQIWKRTMETLLEKYKPDDREFRRPSESARPRVVATPTEEPSEEPSEVPSEDPSD
                     PGGPLPLPTILRPTPPARPG"
     gene            14479..15276
                     /locus_tag="VM841_14180"
     CDS             14479..15276
                     /locus_tag="VM841_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HVL91374.1"
                     /translation="MPAPTIMCSTGPFYMLPLGQAFEAISRAGFEGVELMVTSEPETQ
                     TPERVKAIAEDFGLSVPAVHAPFLLLTRRVFTTDPLEKIKRSADLAQRMGSDLVVVHP
                     PYRWQTSYARWCDEELQGFNDKEEVTIAMENMFPVWIRGRGVAFHRTTGIEDMKKYSK
                     VVLDTSHLAVTGIDIVTAYEELADRIVHIHLSNNLGTGRDSHSPLTQGVLPIGSFLER
                     LGRSGYSGTITLELDVRPWAGQPAKLRSVLKEQRDYIAERLGAPAES"
     gene            15409..15795
                     /locus_tag="VM841_14185"
     CDS             15409..15795
                     /locus_tag="VM841_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL91375.1"
                     /translation="MTVRVMIVDDTDHVREMLASMLELDGFDVVGKASSGPEAIAMVA
                     AADPHIVVMDYKMPGQDGLTTTRRIRSTHPDVPVILYTAYLDDLLQAEARDAGVSVCV
                     GKVEGLETLEREISALCLGVIEGQPA"
     gene            16457..17080
                     /locus_tag="VM841_14190"
     CDS             16457..17080
                     /locus_tag="VM841_14190"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91376.1"
                     /translation="MAEHAVAENPTSEAIGAAGDEDVIALVDRARDGDHEAFASLYDR
                     YVERVYRFIVYRVGGDSALAEDITSEVFLRALRKIKGFTWQGRDIGAWFLTIARNLVL
                     DHFKSGRHRLESVGFEAPQAGGVGDEVVDAEDEALSRISHNDLYKAISRLGDEQQEVI
                     YWRFLQGYSVNEAAAAMGKSDGAIKALQYRAVKALYKLVVVEEGMER"
     gene            17189..18283
                     /locus_tag="VM841_14195"
     CDS             17189..18283
                     /locus_tag="VM841_14195"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HVL91377.1"
                     /translation="MSNNRFDEEAFARMLEGAQVPHDIKADRMKSLVAAIERARPAST
                     LDPAFASALRDKLTADIAVVAEAEKAARVSWRTRAAIWMQAKNDSWRRSLRVVAATGM
                     AALMLGGTSAAFASAHDALPGQWNYGLKRFTESAQLSVTRGPLARGNAKLDAARTRLD
                     EIRSLAAAGNDEASLYVATFGDMDHATIEASELLVEASRLGAGLVPLETLASFTAAQR
                     SRLEAVLIDVPPGARPAARDSLATLVLLGDRIGDIIEGCPCPGNPLEIAPAGSTSGLP
                     CSCAAPAGHPAGVQDREPRAGEEPSPTPTGTGSPAPQPSSPGTDLVPGTDADDPVNDV
                     VDAVNDILDGLGLPGGTPSVVPLPTPTVGL"
     gene            18796..>19920
                     /locus_tag="VM841_14200"
     CDS             18796..>19920
                     /locus_tag="VM841_14200"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HVL91378.1"
                     /translation="MRNGVSWTRRAAAVLSLAVATVVVAGPGAGAASVDTYNAQASGT
                     TLSLSVSLPLGDVVAGILGNAGVKGNTISERISFSNATADVTSTTSKGSALGQVFFGT
                     LDPVILQVSRLVGYQKDKLPEVSSSVSMASQDGVEDMKKDALAAVEIGPVKIGVGEVL
                     SKAKRSGRLVATKSEARLLGIKVDLAAVLSEGAIADLLKPVTDLLDGSGNTPGLIGQL
                     NGLLDGVEQTLKGSGLDVDLTVPKIKDLLNKPLVNVGVIESISETGFNGVNRFAKGIT
                     RLANVDLLGGFIHLDAINIESYASIDGTAAGAVASGIQRIVGLKVGGNVVNLNTGSLQ
                     VLGKTIQMPVELTNLLNLTVDALGLKVQVAQKPEIVRTATH"
BASE COUNT         3296 a         6726 c         6570 g         3328 t
ORIGIN      
        1 gtccggcgtc gtcgttcacc ggcagttcgg gccgctgaac gaagagcagc tgcgcgagct
       61 cgccgcggag catctcggcg tccgctgatg agtccgtttt cccgctccct cggttgcatc
      121 cgacggtcgc gcggcgggat aatcagcgtg caggtcgatt cttttttttg gagtgcgctt
      181 gtgatttcaa gcacaagcgg attttctggg cgatgggctg cgggatcgga acgaaccggc
      241 cactttggcc ccggacacgg ggcacagtat tatccgcggc tgtcgggcac cgacgacacg
      301 gaacgctttc ggaaccgtga gaggcggatc gagtcgatga ggaggacaac gaatccgtga
      361 tcagtccctg gtggctcctc ttggaaggtg tgtacttcgc gttctgcggc gcgctgttct
      421 tcggcgcgta catgtccaag aacgagtgga tgcgcgcctc gctcgcgtct ctcgcgctgt
      481 ccatcatcgc gtggcgcgtg ctcgcggtca ttccgtcctg gtggctgtac tacgcggaag
      541 gccagctgaa gtggggcggc cagggctgcc tccagctcga cctcgcatgc ttgaagcagg
      601 caatcaagga caccatcgtg gtgatccaga acgcggtcgc cctcggcggt ttcgtcgtcg
      661 cgttctggct gtaccagaag aagttcccca agcagctcgg cgccggcgaa gcgaagccgg
      721 aagccactgg aggctataag tgagcgcgac gtccaacaag ccgcagatct acgacgacta
      781 cgagctcaag gtcgtcgacc cgaactggct ggcagagcgc gtcaagccga agcgtcacat
      841 cggcctggac gcggatctgt gcatcctgtg ccgggcctgc gaggacgtgt gcccttggga
      901 ctgcatctac atgctctctc ccgagatcgt tcaggacgcc gagacgcctg cgctcgaagg
      961 agcaacgaaa gcttcatccg cgattttcgt catcgacgac aacgagtgca cgcgttgcgg
     1021 aatctgcgtg gaccgctgcc cgtctgacgc attgttctac gtaaccaccg gagcgaagaa
     1081 gggccgctga agcagcgcgt tgaaggcggg gggccgtggc agaaaaaccg atcctgcaag
     1141 agcagctcga catcctgaaa gacaaggttc aggactctca ggtctggaag tcgatcttcc
     1201 gtcccgggtc gtggctgcgc aagggctaca agggcacatc gcgagaccgc gcgctcgcga
     1261 tgatgaacaa catcctgtac caccttcacc cggtcaagct gaagcgtcac ggcttgaagc
     1321 tgacctacac cttctgcctc gggggcctgt cgttcttcct gttcgtgctg ctgacgatca
     1381 ccggcatcta tctgatgttc ttctatcggc ccgccgccga gatcaccgcg cagctcgcct
     1441 accaggatat ggccgacctg tcggccgcag tcacgttcgg gcagctcact cgaaacctgc
     1501 accgttgggc cgctcacttg atggttctga gcgtgttcct gcacatggcg cgcgtcttct
     1561 accacggcgc gtacaagcct ccgcgggagt tcaactgggt gatcggcgtc aacctgctgc
     1621 agatcacgct gctgctgtcg ttcaccggct acctgctgcc ctgggaccag ctggcgttct
     1681 gggcggtgac cgtcgggacg aacatgatgg gctacacacc ggtgttcggc cgacaggtga
     1741 agttcgttct cgtcgggggc ttggaagtcg gaccaccgac gctcatccgc tggtacgtgc
     1801 ttcacgttct ggcgttgccg ttcgtcgccg tgctgctgat ggccgtgcac ttctggcgca
     1861 tccgcaagga cggcggcatc tcgggaccgc tctaggagat tcggatgtcc gaagaagtcg
     1921 gcatcgatca agaaacattc gataaggcga tcgcggaggg caagcccgag cgggtcgcac
     1981 gggcgctcgc gaagtcggcc tgggttcgca agaaccgcgg cccggcctcg gcggcgcctg
     2041 ctcctgctgc tcccgcaccc ccgggtgacg gtgacggggc cgaagctccg gccgctgccg
     2101 cgccggccgc acccgctgcc caagctccgg ccgctgccgc gccggccgca gcgaccgcga
     2161 ccgtgccgcg cctgactccc gaggagcgcg ccgctcgcgt cgcggccgcg accggaacga
     2221 aggtcaacgg cggcggcgga cgcgggaccg gcgccggcga gacgcaccgc ctgctcgcct
     2281 tcgtcccgcc ggacggaatc cagcgggtcg agaagcgcca ggaggacaag atcaacgtct
     2341 ggccgcacct tctggccgaa gagttcctct cgctggtcat cgtgctcgcc ggcctgatcc
     2401 tgttctcctc gttcgtcaac gcgccgtcgc gcgagctcgc gaacccgttc ctcacgccga
     2461 acccctccaa ggcgccgtgg tacttcctcg gtctgcagga gctgctgcgt tacttccacc
     2521 cgatggtggc aggcgtgacg ctgccgggcc tgggcctgtt cggcctgatg gcggtgccgt
     2581 acgtggacaa gaacccgtcg atgaagccgg agcgccgcaa gctggcgatc atgctgttca
     2641 cggtcttcgt gctgttctgg tcggtgctga ccatgatcgg tgtgttcttc cgcggtccgg
     2701 gcttcaactg gatctggccc tggcagacgg gcctcttcgt ggacctgtga tgaccatgat
     2761 gattgcgaag atctccacta cgaacctcat tctgatcggc atcgccgtcc tggtcggcct
     2821 cgccggcgtc ggcatgatca ccgcgaacat cttcgcgatc atgcgcgcca agaaggacgg
     2881 cccggcttcc cagcgcccgt tgatcggcgc gccgaagcaa ctgtcgcgcc gctcgttctt
     2941 cggccggacg ctgttcatcg gcttcggcac ctcgatgacc gcgttcggca cgctgtcggt
     3001 cggtttcctg tggccaaatc tcaagggcgg cttcggcgcc aagatcaccc tcccccagac
     3061 ggccgaggag atcctcaagc agatccagga gaccaacgag ccgtacttca actcgaacgg
     3121 ccgcttctat ctcgtgccct actcgcagtc cagcgagggc ggcaaccgtt acgcccagga
     3181 gggaacggcg gcgggcggcc tgatggcgct ctaccaaaag tgcgtgcacc tggggtgccg
     3241 ggtgccgttc tgcgcgaact cgcagtggtt cgagtgcccg tgccacggct cgaagtacaa
     3301 ccgtgcgggc gaatggaagc tcggcccggc cgctcgtggc ctggaccgca tggtcatgag
     3361 cgtggagggc ggtatcgtga cggtggacac cggaaacatc atcaccggcc ctccgcgcgg
     3421 caccaacacg accggtcagg aaaaggaagg cccgttctgc gtctagcagg ggcggggttc
     3481 gagggggact ggtgaacatc gtcattttcg cagccaagga tccgcggctc gtcgcaggga
     3541 tcctggtcgc atcggcactc gcgctgatcg cgatcttcct catcgcctcg gcgatgtcga
     3601 gccgccgccg caagtccgag atcccgtggg ccatccggcc cggcccgtcc gacgacgagc
     3661 tcgagtccaa gacgctcccg cgcttcctgg ccgtcgggtc gatcttcctg ctgttcctgt
     3721 ccgtgtggct tcccgggctg tggcttcggg agcccacgcg catcgacctg aaggaggagc
     3781 agttcctccg cgagtcgatc caccgcggcg agatcctcta cgccgagcac gcgcccgagg
     3841 gcacccgcgg ggcgcacttc tcggcgaact gcgcgcgctg ccacgggccc gagggcgaag
     3901 gcgcgctcca gcccttcaag gagaagttcg actacgccga gcctccgctc aagtacatcg
     3961 tctcccgcta caaggcggcg ggcaacaacg acgaggagat ccgccagatc atcgtcgacg
     4021 cgatcgaacg cggccggccc ggaaccttga tgccgacgtg gggtcagtcc ttcggcggcc
     4081 cgctcaacca gcagcagatc gacgacgtcg tcaacttcat ctacgcgatc caggagccga
     4141 tccccggggc cggtgacgtt ccggtcaccg cggactcggg ggcgcagctg ttcgacgcga
     4201 actgcgccgt gtgccacacg cacaaggacc cggagacggg cgagattgtg tggggcggcg
     4261 tcgggcccga cctgcgcggc gtggtcgcgc agctcggcgt ccaaggggtc cgcgacacga
     4321 tccacgacgg ccggatcaac tacaaccgtc cgtcgatgcc cacgtgggcg ttcctcggcg
     4381 attcggcgat cgacgcgctc gtcgcgttcc tccagagcct gcagacggcg aggtgaccca
     4441 ggtggaatcg ttgcttcagt tcgcaccgtt cgcgctcgag ggccagtggt tcggcgccgc
     4501 ggccgtcgcg ctcgtaatag tcgggatcat catcttcatc ggaacggtct acatcctgct
     4561 cgtggcgatc tacggctggc ttcaggcgta cctggtcacc atggtgtctc tgatgatctt
     4621 ctcgatcatt ctggccgccg tctggctctt cggggttccc ggcacgccac cgggcaccgg
     4681 tccgcgagga acagagccgt attgggtggt cttcctgccc gatagtgagc agggcttgga
     4741 gttcaaggaa gagatcgaga cgttcccggc cgaccgaagc gtcgcggttc ccgaagcggg
     4801 cccggtcggg gacacggaat ggcgcatcgc gcttccggac gagcagtatc ccggcgcgat
     4861 caacgtcgac ggcgagatcg acacgatcaa ggtcgtgttg cagccggcgc ttgcggggta
     4921 cttccagcag cagggcaccg gctccggcaa gccctccgac tactcgttcc ggctcgaagg
     4981 ccgcgctcct tcattggagg aagaggcctt gcccgtggcg cggctgctgt tctacccgaa
     5041 cggcgacgta gccaaggtcg atccggggac gcaagcggtc ctcgagaccg atcgcatcgg
     5101 cgatccggcg agccggttgc tggccggcat cgacatcccc gcgaccgaga accacccggc
     5161 gatccgcgtg ttcgcctacc gcaacaaggg tcaggtgttc atggcctcgg ctcagtggat
     5221 ggttgtgtcg gtgatcttgt tcgtggtcca cctgctgtgg ctcgcaagct acgagaacaa
     5281 gcagaagcgc cgcgatgccg agcttgccgc gggcacaacg ccgccgcgcg agccggtcac
     5341 gaccggctga tcacaggacc gaaacgaaga agcgcccgga aattgccggg cgcttcttcg
     5401 tttcccggcc actacgaatc gagcggctcc atggcgagga gctcggccgc tccggcgtgc
     5461 atcatgcggt ccgcgagcga ctgtccgacc ttgaccggat cgtcgccgat ctcgatctcg
     5521 cggatgatgc ggccgccgtc cggcgaacac acgatgccgc gcagcttgac catcggaccg
     5581 tcgagcttgg cgatcgcggc gacgggcgcc tcgtggtcgc cgcccatctc caggaagaag
     5641 gtgcgctcgg cgtcgaaggc ccggcgggac agctcgtgct ggacctggcc gagcgcggcg
     5701 cgcatgtcct tgtcgttgat gcggcactcg accgccaaaa acccttggcc ggccggaggc
     5761 atgaccatat cgatcggcag gatctcgacc gcgcggcttt ccctgccgag ccgcttcagg
     5821 cccgcgatgg gcagcacgag cgcttcgatc tcgccggcgg tgagcgcgcg cagccggatg
     5881 tcgagcgccc cgccgatccg caccggcgtg aggccgcggc gcatcgccag aacctgcgcg
     5941 gcccgcagcg ccgaggtaac gccgacccgc gtgcctggct tgagcgagtt caggcccgag
     6001 ccgcggcgag tcacgagcgc ctcgcgcggg tcgatgcgtt cggtgatcgc cgacagcgcc
     6061 acctgggcgg gcccggtcac cggcagatcc gaggcattgt gcacggcaag gtcgatcttg
     6121 ttgtcggcga tccccttgcg gatctcggcg atcacctgcc cgcggccgcc cggctccccg
     6181 gcctcgtcgg cgtcgttggt aaccggcacc agctcgaagt tccggcgcgt gatgatctgc
     6241 tggacggcgc ggcgcaccga ctcggcctgg gctagcgcca cggcgctggc ccgcgtcccg
     6301 attctcaaca cgcttcgcgg cacgattgtc tcccctcgct cggctgcgcg actcgtgccg
     6361 attctcgcac cagcagggcg cggatgtggc cgcttccgag aagaatttga actaattttg
     6421 tccggccggc gcgccggaca cctgcgccgc acgacggtcg cagcggcccg gcgcgatggg
     6481 cggcttcgcg tcgagggcgc ccccggcttg gtgcggctct cgcgtcatgg ttccggccga
     6541 gcccttacgc gggaaacccc gcagccgtcg atgactaagg gcgggtggac ttggcggcgc
     6601 tgccggtgag cttgcggggc gcgccgacgc tttccagcgc ggacttgcgc tgctcgtaga
     6661 acgacaggat ctgaagctcg atcgacaggt ccaccttgcg aagcgacacg tcgtcgggga
     6721 cttggatgac ggccggcgcg aagttcaaga tgctcttcac gccggccgac acgagacgct
     6781 ccgcggcatc ctgcgcagcc ggagccggcg tggcaatgat gccgatcgac acctgacgtt
     6841 cctttgcgat tcgttcgagg tcttcgaagt gctcgatggc gagatcgccg atcttttcgc
     6901 cgatcttgcg gtcgtcggcg tccacgagcg cgacgatacg gaacccacgc gccgagaagc
     6961 cgcggtagtt ggcgagcgcg tggccgaggt taccgacacc gacgatcacg cagttccagt
     7021 cctgggtcag gccgagctcg cgcgagatct ggtagatcag atactcgacg tcgtagccga
     7081 caccgcgcgt gccgtaggag cccagatagg acaggtcttt gcggaccttg gccgagttca
     7141 ccccggccgc ctcggccagg ccctcgctgg agatcgttgc catgccgcgc tcatgcatgt
     7201 ccaccaggct gcgcagatac aggggcagcc gggcgacggt ggcttcgggg attgagcgtt
     7261 ctctcatgcg aaagcggcct ccggaacgcc tccactgtaa acgcttgtga gcacattctc
     7321 aaactatcga tgcgctggca cgccccctga cctgcggaaa cgcgggtgct tgcgttgctg
     7381 gccgccgact aagcgcgctt gcgaagcgcg cggcgcacgg cggcggcatc catcggcccc
     7441 tcggctcgct cggaaccgtc cacctcgagg atcggggtcc ggatgaaacg gtcggggtcg
     7501 ccggcggcat caacctcttg gaacgggatt cgcatcgccc ggagcacccg ccgcggctcc
     7561 tcgcacaagt gacaatccgg ggtgacgtag agcacgacgt tcctgggtgt ccgcgatgcg
     7621 agcacgaagc cgacgaacat caccgtgacg ctcagcgcga gcaccaccga gaacgggccg
     7681 ccgcgtccct tgccgatcag cgcgaagccg gcgaacgcgt tcagcgtcac cccggcggcg
     7741 atgatccagt tgccccggat gcggtcacgg ggcgggcgga ccccgtcgcg tttgcgaccc
     7801 gagaccgccg cgatccccac cacgatcgcg aacgacggca aggagtacgt ccgtgccagc
     7861 ccccgcgcgg gctcgcacgg ctcgatgatc tttctccaac cgggatcggg gcaccagact
     7921 tcgctgcctt tcggaatctg ctcggtccga agcgcttgtg cctctaccga agacgaccag
     7981 acggcgacga agccccagat cgtcccgacg acgaccatca ggagcgaggc gagcgtgatg
     8041 gctcgcttgt tcaaaagcgc gatcgacccc agcgccagcc acgggacgtt gagcatcgct
     8101 ccgaagaccc aatacacgcg gtacaacgtc gggtcccagc caccaccgat gccggccgcg
     8161 accgcgaccg aggcgatcgc gtactgcgcc agcgcgaccc cccaggccag ctgcgacagt
     8221 cgtttgcgcg accggtactg ccggaagagc gcgaacgaga agacggcaga gatcagcgcg
     8281 gcgatcaggg gaaagacgat catctagctc tcgcgggcgc gcaacgtgcg cttggcgacc
     8341 ttgcccgtgg gcaggtgcgg cagctcccgc acgaactcga tcacttcggg cagcttgaag
     8401 cgcgcgagcg accggcggca gaaatcgagc agctcctctt cgctcgcgag ttcgccctcc
     8461 ttgagcacga ccaaagcttt caccgcctcg cccgtgtacg gatgcgggat gccgatggcc
     8521 gcgcactcgg cgaccttggg atggcggtac aggacgtttt cgatctcgcg cggatagacg
     8581 ttgaagcccg agacgatgac cagatccttc ttgcggtcga tcaggaagat gtagccgtcc
     8641 tgatccgcgt agccgatgtc gccggtgcgg aaccagccgt cctcggtgaa cacgcctttg
     8701 gactcttcgg gctgcctcca gtacccaccg aagacattcg tgccgcgcac cgcgatctct
     8761 cccgggtcgc cctcttcgac gtcctctccg tcctcgtcga tgatgcggac ctcgacgccc
     8821 ggcaaggcct tgccgatcga gccggccttg gcgacgtcgc ccatcgcgtt gctggtcaca
     8881 ccgggcgcgg tctcggtcag accgtagccc tcccagatcg tcacgccggt cttacgcttg
     8941 aagtcctcaa agacctgggc gggaagcgcg gcagcgccgc tgaccgcgac gcgcacgctc
     9001 gacaggtcgg cacgctcgac gccgggcgta ttgacccagg cgatgaacat cggcggtgcg
     9061 ccgaacagga tggttacctt gtgcttttcg atcgcctcca gcgagccgac cgagtcgaag
     9121 cgttccatca ggacggccgt cgcgccgacc ttgagcgtca gacccaggat gacgttcagc
     9181 gcatagatgt ggaacagcgg aagcacgagc agcaccacgt cggtctcggc ttcctgcagc
     9241 agcggcaccc ggctcatctg atccaggttc gccagcaggt tgccatgcgt gagcatcgcg
     9301 cccttgggct tgccggtcgt gccgctggtg tagcccagga ccgccaggtc ccccggcttg
     9361 gtatcgacct tgggcggcgt ggcgctgcgc gtcgtgaact gcccccaggt cagcgcgccc
     9421 atcggcgcgc gcgggccggt cacgaccaga tgctcgagca tctgcgcggt gtggcgcacg
     9481 ccgtccacca agggggcgaa cggctcggcc gcgatgacca cgcgggcctc ggagtcggtc
     9541 aggatgtggg cgatctcctc gccggtgaac atcacgttga tcgggaccat gaccgcgccg
     9601 gcgcgcatca cgccgtagta ggcggtaatg aacggcggca cgttgtgaac gagcaccgcg
     9661 acccggtcgc ccggccggac cccgagcgag gccagtccgg ccgcaactgc gtcgatctcc
     9721 tcgtcgagct cgcgataggt gatcgacaga tcctggaaca gcacggccgg cttgtcgggc
     9781 aggcgctgag ccgtcgcgcg cacaaaatcg gaggcattca tacagggcgg aatcctaatc
     9841 gggggtgccc gactcgggcg atttcatgcg ttggcgatgt cgcgagcgtc gaggttgcgg
     9901 agcgcggtgc cgttccgcgc ggccagcgcc gcggcccgcc ccgcggcctc gccttgagcc
     9961 atgctcggcg cgatcacccg gctggaggcc agcgcctcgt gggtcgcgct gatgcagcgg
    10021 ccggccagca gcaggttgtc ggcgtcccgc gcgagcagcg cgcgatacgg gacctgatac
    10081 gacgcgcccg gctcgagcca gacgtactcg gttcctttgc cggcgacgtg gaactcctgc
    10141 ggccaggcgc cggcggcgac ggcgtcgtcg aacggggcgg ccgacaggac gtcctcgccg
    10201 gtgagcacgt agtccccgac cccgcggcgg gtctcccgga tgccgagctg aggcggggtg
    10261 tcggcgatga aggcgttcgc gaagccgggc atgtgcgcga tcaggaagcg ggccgccttc
    10321 tcggcctcgt cccgcccgtt catctcggcc acggcggcct cgaacggatc ggtcatatcg
    10381 ggcgagcgcc cctcgacgga cacgcgggtc atcgccccga ttgcctcccc atgacggaac
    10441 gtgggaagaa cggcacctcc cgaacgtgac aggttccatt cgggcgtttg tccgatcgtt
    10501 gacaacagtt cgttcagctt gtccaatccc gccgcgtagg cggcttgcac gtccacgttc
    10561 tccatcagga actgcatcga ggggaactga cgctggccgg ggccgccggt ctcggttgca
    10621 caacccgcgt ggaaggcaag gtccgcgtcg ccggaggcgt ccacgaacac cgcggcggtg
    10681 atcgccagcg gcccgcgctt cgatccgacg acgaccgcgt cgatgatccg tccggatcgc
    10741 accacttcgg tcacggtcgc gtgcaaaagc ggagtgatgt tcggctcggc acgcaccatc
    10801 tcgtcggcga gacgcttata ggcccagggt acgtacagca gaacggcggt ttccttgtac
    10861 ggaacgggac cgaatgcgga acccgttgtt ttcaggcgtt ccgcccactc cctagggaat
    10921 ccgtcgtaga ggtcgcgatg ctcgtcgccg tctcgcacgt acaagccgca tatcgttccg
    10981 acgtatgcgg cgaccgcatt accgccgaag aatccatagc gctcgatcag cgcgacccgc
    11041 gcgccggccc gggcggcggc gatcgccgcg gccagtccgg ccgacccgcc gccgcacacc
    11101 acgacgtcgc aatccagcac gatcggagcc gtgcgtacgt cgaggcgaac ggtgcgagcg
    11161 tccatctgct tcgaaaagtc taggcggtct ttagcccgtc ccgtccagtt tcggggcgca
    11221 aattccgctc aaggggcctg cggacggtcc cgatactctc agtgacacat tgacaaccga
    11281 gcacgggccg taatccacga caagggcaaa ctcgccggga ggcgaggacg caaagccacg
    11341 ggaccgacac gcgtcagcga aggtcagccg ggttgccgaa aggaacggtg cgatcgcttc
    11401 accggtcgcc cgtccgataa ggcgcagggg ggaaaacctg cagtggacgg gccgcggtgc
    11461 tcgagctaac cgacaggggg ctcgagaggt atgaagcgat ccatagctgt gttcgtcgcc
    11521 gtctgcatga cggcaggagt cttgttcgtc accgcctcgc cggccgcggc gctttcctcc
    11581 tacgagaacg acttcgcctc gcgccacaac tcgatccgcg cgtctcacgg acgcaaggcg
    11641 ctggccgtct acggcgatct cgttgacatc gcccggcgtc actccggccg gatggcctcc
    11701 gaaggccgga tctggcacaa ccccaacctg acctcggaag tgaagaactg gcaggtcgtc
    11761 ggcgagaacg tcggcatggg cccgagcgtg cccgatctga tggacgcgtt catgaactcg
    11821 ccgggtcacc gcgccaacat cctcgacggc gagtacaacc agttcggtgt cggcgtcgtg
    11881 gtcaaggacg gcaccatcta cgtgacggtg gtgttcgcgc ttcgctcgag ctcgagcgga
    11941 tcgaccacgg cgaagaagcc gtcgtccacc acgaccagca agagcagcac catctcgaag
    12001 ccgcgcagca ccgcaacgag tccgcgcagc accacgacga agcccgcgtc caagccggcc
    12061 gcggcggcga gcgctcccgt cgctcccgct ccggtcgcgg tcccggtcgc gcctccggtg
    12121 cgcacggtcg acatgctggt gcggatgatc cgaatggatt cgtagccacc ggggccgcgt
    12181 ccgggctcat gccgcggacg caccctcgag ccctcgggac accccctgac ccgggggctc
    12241 actctttgcg acggtccggc gctcgacgcc ggaccgttcg ccgatccggc gtctacccgg
    12301 gccgggccgg cggcgtcggc cggaggatgg tcggcagcgg cagcggcccg ccggggtcgc
    12361 tcgggtcctc ggacggcact tcgctcggct cctcggacgg ctcttcggtg ggcgtcgcga
    12421 cgacgcgcgg gcgcgccgac tcggacggcc ggcggaactc ccggtcgtcc ggcttgtact
    12481 tctcaagcag cgtttccatc gtgcgcttcc agatctggag cgggaacgac gagccgacga
    12541 cgttgatccc gtgcacgcct ctcatgggca cgcggccctg gggatggccg acccacacgg
    12601 ccgcgaccgc gcttccggcg ccgccgacga accaggcgtc ggcgaagtct tccgtggtgc
    12661 cggtcttgcc ccaggtcacg taatcgggga tgcgcgcctt gcgtccggtc ccgatgcgca
    12721 ccacctcctt catggcgtcg agcacgtcgg aggcgacgtc gaaggtgacg gcgcgccggc
    12781 cgcttcggtc accggccttg cgattccgat agagcatgtc gccctcgcgg ttcttcacct
    12841 cggtcaaggc ggtggtcctg agataggtgc cttcacgcgc gaaggtggca tagccggtcg
    12901 ccaggtccat cgccgtgacc tccgacgtgc cgagggcgag cgactcgacc gcgcgcagcg
    12961 acgcggaaac gccggtcgcc ttggcgagct tggccacgtt ggacgcgccc acgtcgcgga
    13021 tcagttgcgc gaagacggtg ttgacgctgt aggccagcgc gcgtttgagc gtggggcggc
    13081 cgtatccgcg gcggtcgtag ttgccgacct tccacacggt gccgttgtcc aggcgcagcc
    13141 gcatcgaccc gggcgccgag tagcggtcgt cgagcgtctt tccctgcttg agtccggccg
    13201 ccagaacgaa gggcttgaaa gccgatccgg cctgacgaag accttgcacg gcgagattga
    13261 ggtcgcccag tcgcttgccg cggccgccga ccatcgcgcg cacgccgccc gtctcgacgt
    13321 cgatgaccac gacggccgcc tcgggatcgc ccggctcgtc gagcagctcg gcgatcgcct
    13381 cctccgccgc acgctgcacg tcgaggtcga gcgaggtcgt tacgcggagg ccgccgcgat
    13441 acagcacgtc ctcaccgaac tccccctcca gaaccttgcg tgcccagtcc acgaacgacg
    13501 gcgcgatcgc gacctgcttg accggcggcg cgatgtcgat cggcatggcg gccgcgaacg
    13561 tctcctcctc gggcgtgatc atcccgagga tgcgcatccg ttccaggacg tagttgcgcc
    13621 ggaccttggc gccgtcgggg ttctttcgcg gatcgaaccg gcccggtgaa gcggtgatcc
    13681 cggccagaag tgccgcctcc tccagcgtcg cctggttgat gtcgtggccg aagtacagac
    13741 gcgacgcggc catgacgccg tacgcgccgt tgcccagata gatcgagttc agatacgcgg
    13801 cgaggatctt ctccttggag aacttgcggt cgacgcggat cgcggtgatt gcctcccgga
    13861 tctttcgcca gatcgttcgc tcgttgccga cgaaggtgtt cttgacgtac tgctgggtga
    13921 tggtgctcgc gccttcgcgg tcgccttggg cgttggcgaa cagggcgcgg gcgatcgcga
    13981 tcggatccac gccccaatgg ctccagaacc gttcgtcttc gaccgccacg aaggcctcct
    14041 gcaccacctc gggtatctcg tcgatgccga ccacgactcg gttctgctcg ggccgaagct
    14101 ccgcgagcgg ccgtccgtcg atgtcgagca aggtcgcgtt ctggaggggc ggcgcggccg
    14161 aaagcgaggc cgggccgaag tagatgaccg acgatcccac cgcgaagatc gtcaggccgg
    14221 tgagcaggac gagcttccat ttgcgcatcc gtccgaggcg gcgcagcacg cggacgggca
    14281 ggcggcggcg aaccccgtgc ggctcgggcc ggcgcgtcgg tggggggatg tcccgtggag
    14341 caagcgcgcc ttccgcaagc gccgttgagg tcgtggacgc cttagggcgg cgctcgtcca
    14401 tggaacgatt gtagcccccg aatgcccgcg cgttccctac aatggccgcg gtcggccgat
    14461 tcccctaaag gagccctctt gcccgcaccg acgatcatgt gctcgaccgg gcctttctac
    14521 atgctcccgc tcggtcaggc attcgaagcg atcagccgcg ccgggttcga gggcgtggag
    14581 ctgatggtca cctccgagcc cgagacgcag acgcccgagc gggtcaaggc catcgccgag
    14641 gacttcggcc tttccgttcc cgcggtgcat gctccgtttc tgctcctgac ccgtcgcgtc
    14701 ttcacgaccg acccgctcga gaagatcaag cgctcggcag acctcgcgca acggatggga
    14761 tccgatctcg tcgtggtgca cccgccctac cgctggcaga cctcgtatgc ccgatggtgc
    14821 gatgaggagc ttcagggttt caacgacaag gaagaggtca ccatcgcgat ggagaacatg
    14881 tttcccgtgt ggatccgcgg gcgcggggtc gccttccacc ggacgaccgg catcgaggac
    14941 atgaagaagt actccaaggt cgtgctcgac acctcgcatc tggcggtgac gggcatcgac
    15001 atcgtgacgg cctacgagga gctcgccgac cgcatcgtgc acatccatct gtcgaacaac
    15061 ctcgggacgg gtcgcgactc gcattctccg ctcacccagg gcgtccttcc gatcggctcg
    15121 tttctggagc ggctcggtcg cagcggctac tccggaacga ttaccctgga gctggacgtg
    15181 cgtccgtggg ccggccagcc ggccaagctg cgctcggtgc tgaaggagca acgggattac
    15241 atcgcggagc gcctcggagc gcccgcggaa tcctgatccg cgtcgcgccg cagaagcttt
    15301 tctcgaccgc tatgccggtt ttgcaacggt cctgccgtcc cgggcgctca agaggaggac
    15361 gagcgtggac gattcatcag cagacaagta ttcggagcgg agaaaacgat gacggtccgt
    15421 gtgatgatcg tggacgacac cgatcacgtc cgcgaaatgc tcgcatcgat gctggagctc
    15481 gatggcttcg acgtcgtcgg caaggcatcc agcggcccgg aggccatcgc gatggtcgcg
    15541 gccgccgatc cgcacatcgt cgtgatggat tacaagatgc ccggacaaga cgggctcacc
    15601 acgacccggc ggatccgttc gacccatccg gacgttcccg tgatcctcta caccgcatac
    15661 ctcgacgacc tcctgcaggc cgaggcacgg gacgcgggcg tatcggtgtg cgtcggcaag
    15721 gtcgaaggcc tcgagacgct cgagcgagag atcagcgcgc tgtgcctggg cgtcatcgaa
    15781 ggccagccgg cctagacacc caccgaagcg atccctcgcc cggaccttca agaaggcggg
    15841 ccgtcgggcc cgccttctcg atggtggaga agcctgtgga tcaaaaccgc ttccgatgaa
    15901 acggccggtg aagcgaagca gtgaatcgag ggccgcgcag ttgcctcgcc cttcatccct
    15961 cgcccgagcc aatttccgat gccggcttca ttcctcggac ggcaacactt tgggcgctgc
    16021 cctcactttc ctaactacgc ggccggcgaa gggtttcctt cgccggcgag gcggctcgct
    16081 tcaaagttcc gttttcccgg tattgcccgc cggcctgggc ctgcccggcc tcccgaatcg
    16141 aagccgagcc cgcccggctg tcgcagacgg ggcgcggcgt cgttctgact ctgcgacggg
    16201 cgatgcggac cgaccgcgga gctcgtccga ggttcgcggc aaggtgcgag caggcctcac
    16261 gtgcgctacc gtcgctcacg acatgcgtgg atggacggcg gcgacgctcg tggtgcttct
    16321 cgtaacgctg cttaccgcgg tagcggtgca gttgattacg aatcggtagt agcacgaaag
    16381 agcgacgaca atgcctcctt cctctcgggt caactctgcc ctcgccgcgg tcgatagaca
    16441 cggcgaggag atcatcgtgg ccgaacatgc cgttgcagag aacccaacat ccgaagcaat
    16501 cggcgcggcc ggcgatgaag acgtcatcgc actcgtcgac cgggcacgcg acggggatca
    16561 cgaagctttc gcgtcccttt acgaccggta tgtcgagcgc gtgtaccgct tcatcgtcta
    16621 ccgcgtcggg ggcgactccg cgctggccga agacatcacc agcgaggtgt tccttcgcgc
    16681 cctgcgcaag atcaagggct tcacgtggca gggccgggac atcggcgcct ggttcctcac
    16741 gatcgcccga aatctggtgc tcgatcactt caagagcggc cgccaccgct tggaatccgt
    16801 gggcttcgag gcgccgcagg ccggcggcgt cggcgacgag gtcgtggatg cggaggacga
    16861 agcgctgtcc cgcatctcgc acaacgacct ctacaaggcc atctcgcggc tcggcgacga
    16921 gcagcaagag gtgatctact ggcgattcct tcagggatac agcgtcaacg aggccgccgc
    16981 cgccatgggc aagagcgacg gagcaatcaa agccttgcag taccgggccg tgaaggctct
    17041 ctacaagctg gtcgtcgtcg aggaaggcat ggaacgatga ctagtcctta cgaatcacgt
    17101 gaaagaccac ccgccggact tcgtgtccaa agcgggcaat gactcgttgc gcaccgtgag
    17161 ggacggtcta cgcagaggga cggaagccat gagcaacaac cggttcgacg aagaagcctt
    17221 cgccaggatg ctcgagggcg cgcaagtgcc gcacgacatc aaggcggacc gcatgaagtc
    17281 cctggtcgct gccatcgaac gagcgcgtcc cgcttccacc ctcgatcctg cgttcgcctc
    17341 ggccctgcga gacaagctca ccgcggacat cgccgtcgtc gccgaggccg agaaagccgc
    17401 tcgcgtttcc tggcggacgc gcgcggcgat ctggatgcag gcgaagaacg actcctggcg
    17461 ccgctcgctg cgcgtggttg ccgcgaccgg catggccgcc ctcatgctcg gcgggacctc
    17521 ggccgcgttc gcgagcgcgc acgatgcgct gcccggccag tggaactacg gtctcaagcg
    17581 cttcaccgag tcggcgcagc tgtcggttac gcgcggcccg ctcgcacgcg gcaacgccaa
    17641 gctcgacgcg gcacgcaccc gcctcgacga gatccgcagc ctggccgcgg ccggcaacga
    17701 cgaggcatcg ttgtacgtcg cgacattcgg cgacatggac cacgccacca tcgaagcctc
    17761 cgagctgctg gtcgaagcga gccgcctcgg agccgggctc gtcccgctcg agacgctcgc
    17821 atcctttacc gcggctcagc gatcgcgctt ggaggccgtc ctgatcgacg tgccgccggg
    17881 cgcacggccg gccgcacgcg actcgctcgc gaccctggtt ctgctcggcg accgaatcgg
    17941 tgacatcatc gaaggatgcc cgtgcccggg aaacccgctc gagatcgctc ccgccggatc
    18001 cacgtccggg cttccctgct cgtgcgccgc tcccgcgggc catccggccg gagtccagga
    18061 ccgtgagcct cgcgccggcg aggagccgtc cccgacgccc accggcacgg gctctccggc
    18121 cccgcaaccg tcgagcccgg gaacggatct ggtgccggga accgacgccg acgacccggt
    18181 caacgacgtg gtagacgcgg tcaacgacat cttggacggt ctgggtcttc cgggcggtac
    18241 gccctcggtg gtgccgcttc cgaccccgac ggtcggcctc tagcccggat ttttttcggc
    18301 gtcgcgaaag cccgcggcgc ccggtccgaa cccttccgcg gctgcgccac acctgagaca
    18361 ggtgatcgct gccgcccagt tcctccctat atgggacgac ttgcttgccc atattgagag
    18421 catcccgctg ttcaggatcc cggagcaatc acccatgcgg ctcatggggc ccatgtccgg
    18481 attgccgtca gcgaaccccg tacggctagc caaaggtcag aaaaccaggc atactcccgt
    18541 cacctggcag cagtttcttc gttgaggact gtgcaaggca ggaacgcttg tcggcagcga
    18601 gcggcgtccg gggtccggct ggggacaacg gagtccgcga ggtcagacag ggtgggcggg
    18661 aggtgtgtgc ggacgcactt gaatgcccga aattgtcccg cagggacagc tctctctgac
    18721 ctcgaaacat gtaggcgctg acggcgggcg gacctgattc gggttcgtgg ttgggcctct
    18781 acaggagggg gcaacatgcg caacggggtt agctggactc gccgtgcggc ggcagtccta
    18841 tcactggctg tagcgacggt tgtcgtcgcc gggccgggtg caggcgcagc aagcgtcgac
    18901 acctacaacg cccaggcgag cggcaccacg ctcagtcttt ccgtgtctct cccgctcggt
    18961 gacgtggtcg ccggcatcct cggcaacgcc ggcgtgaagg ggaacacgat ctccgagcgg
    19021 atttcgttct cgaacgccac cgcggacgtc acctcgacga cctccaaggg ttcggccctc
    19081 ggtcaggtgt tcttcggaac cctcgacccg gtcatcctcc aggtctcgcg tctggtgggc
    19141 taccagaagg acaagctgcc cgaggtttcc tcgagcgtgt cgatggcgtc gcaggacggc
    19201 gtcgaggaca tgaagaagga cgccctcgcg gcagtcgaga tcggcccggt caagatcggc
    19261 gtcggtgagg ttctttccaa ggccaagcgt tcgggccgtc tcgtggccac caagtccgaa
    19321 gcgcgtctgc tcggcatcaa ggtcgacctg gcggcggtcc tgagcgaggg cgcgatcgcg
    19381 gacctgctca agccggtcac cgacctgctc gacggctcgg gcaacacgcc cgggctcatc
    19441 ggccagctga acggtctgct ggacggcgtg gagcagacgc tcaagggctc gggtctggac
    19501 gtggacctga cggtcccgaa gatcaaggac ctgctcaaca agccgctcgt caacgtcggc
    19561 gtcatcgagt cgatcagcga gaccggcttc aacggcgtca accgcttcgc caagggcatc
    19621 actcgcctcg cgaacgttga cctgctgggt gggttcattc acctggacgc gatcaacatc
    19681 gagtcgtacg cgtcgatcga cggcacggct gccggcgcgg tcgcctcggg catccagcgc
    19741 atcgttggtc tgaaggtcgg cggcaacgtc gtcaacctca acaccggctc gctgcaggtg
    19801 ctcggcaaga cgatccagat gccggtcgag ctgacgaacc tgctcaacct gacggtcgac
    19861 gcgctcggcc tcaaggtcca ggtcgctcag aagccggaga tcgtccgcac cgccacgcac
//
LOCUS       DATLFO010000351        16213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_78648, whole genome shotgun sequence.
ACCESSION   DATLFO010000351 DATLFO010000000
VERSION     DATLFO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78648"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VM841_14205"
     CDS             <1..250
                     /locus_tag="VM841_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91379.1"
                     /translation="FEVGELVTVRSYAGHRADEEPRVIVVGGKEYPVESIDWRAIEER
                     LDGSRRQIFVVRAGGVRIRLASSEESSRWEVERVLTAN"
     gene            337..1224
                     /locus_tag="VM841_14210"
     CDS             337..1224
                     /locus_tag="VM841_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVL91380.1"
                     /translation="MSWIERVSRRRERRAADRAKERWNGPRTAFVLSGGGVLGAVQVG
                     QLRALIEAGITPDVVVGTSVGSINGAMIATDPSADAIDKLTEIWTGLRAEDIFPGSRV
                     ARVWNIVARGDHIHPNDGIRRLIDMLPAHTFESLSLPLHVCAAGLADGEERWFDSGPL
                     ARAILASTALPGVYPPVSIDGNLYVDGGVVNNIPISRAAALGARRIYVLTCGAPNARP
                     RDVRRPIDVLIQSFAHSRAVRFELDKERLKNEAEFVMMPTFDVGAIRYNDPSHSTELI
                     DRAYEMSAEFLAQPGEANA"
     gene            1318..1506
                     /locus_tag="VM841_14215"
     CDS             1318..1506
                     /locus_tag="VM841_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91381.1"
                     /translation="MPEHRLLTYDDLLSFPDDGLRRELIEGELIVSPSPYTRHQVLSG
                     RLFLAFANHLASAGGASV"
     gene            1653..3164
                     /locus_tag="VM841_14220"
     CDS             1653..3164
                     /locus_tag="VM841_14220"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HVL91382.1"
                     /translation="MADPATVERFRELQRKFSDLWPTLTVRAQPIPRTVVIVHSINLE
                     LPPHLLPVVSAYEERYLALVLLLLRQPAGRVIHVTSQPLLPQLAQYYFGLMPKLIRPS
                     SWDRFVPVSVSDGGPGPLTQKLLDRPLTIERIRSHIPDPDWAYIVPFSVGPRERELAV
                     RLGIPIYGTDPDLSWMGTKSGSRRMFAEAGVPHPAGVEDVHSIGDVVEAIAQIRSRKP
                     DVAAVMVKVNEAAGGIGNGVLDLRGADDRAVVEKRVRAVALDDPDGSRDAFFDQLART
                     GGIVEERLAGEEVTSPSVQIRIAPDGVAEVLSTHDQILGGATGQMFLGARFPADPGYA
                     AEIARHALAIASRLADAGALGRFGIDFVCTRNGGGMWDVNAIEINLRNGGTTHPFLTL
                     SALCDGTYDAETGTFTGSDGRRKFYVASDHLESEAYKRLTPDDVLDTIEERDLGWDVE
                     RCTGYAFHLISAVAVAGRLGLTAIADSPAEAQQMSQRAEATFDEISAASQPAR"
     gene            complement(3198..4433)
                     /locus_tag="VM841_14225"
     CDS             complement(3198..4433)
                     /locus_tag="VM841_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL91383.1"
                     /translation="MIDAPVRLGLRQNAAQFGLLVGVNALVGGMLGQERTVLPLLADR
                     VFNVRAFTAVATFVIAFGIVKALTNFVAGALADRIGRKPVLVAGWIIGLPVPIMLILA
                     PNWWWVVAANALLGVNQGLTWSTTVIMKIDLVGPARRGLAMGLNEAAGYIAVAVTAVA
                     TGLIASRYGLRPGPFLLGLSYAALGLGLSTVFVRETHGHARHEAAAHAGAHDGGMSTR
                     KVFALTSLRDRALSSCSQAGLVNNLNDGLAWSLFPLFFARGGLSVAAIGTLAALYPAV
                     WGLGQLVTGALSDRTGRKPLIAGGMFVQAAALALFAATRGFAPWAGAAVLLGIGTAMV
                     YPTLLAAIGDVAHPQWRASAVGVYRLWRDAGFAVGALLSGLIADAFGLAAAIWVVAAV
                     TAASGLLVTIRMYETRASA"
     gene            complement(4430..5770)
                     /locus_tag="VM841_14230"
     CDS             complement(4430..5770)
                     /locus_tag="VM841_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVL91384.1"
                     /translation="MIEIVAFPDEGLGNSSYLIDGGDGSAIVVDPVRDLEPYRRGARE
                     RGLKLAGAIETHLHADFVSGAGALAADGITVAVPAAAAPAFPHRPLADGDELPLGDAI
                     VRAIETPGHTPEHLSYLILRDARPVGLLSGGALLPGTVARTDLIAPEETEPLARRLHR
                     AVHERIAPLPDDVIVYPTHGGGSFCSVAATGERTTTIGRERAANPVFTQSEDDFVAGL
                     LAGLGSYPPYFLRLREVNRRGASSDGPLSLDRLAPDDAEAAMRGGAELIDVRSARAWA
                     DAHIPGSLSIELRPAFASWLGWLVDRDRPLLFVIDDAQSRGELIRACRSIGYDNLIGE
                     IDGGIDAWRDAGRALSRVEVVTDPVVDGPLLDVRQDSEFTAGHVPGALHVELGALADA
                     PTLPPSLTVMCGHGERATTAASVLERNGHRGVRVLAAGPGDWHRTTGMPLERGA"
     gene            5973..6260
                     /locus_tag="VM841_14235"
     CDS             5973..6260
                     /locus_tag="VM841_14235"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVL91385.1"
                     /translation="MYGTVFHFQAKPDAADDVKALFAEWNRDVKPTVDGALNGFLYQL
                     DSDPDRFIACAVFQDKASYMKNANSPEQDKWYQRFRDLLTGDPDWNDGQIS"
     gene            6683..7537
                     /locus_tag="VM841_14240"
     CDS             6683..7537
                     /locus_tag="VM841_14240"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HVL91386.1"
                     /translation="MAPTLLIARNPDPDSSLPYIMRVPIGGGLVLLVRDTWPRTAAVY
                     CHPAPEWPAGAEIVEEIPVRSCTRRGPAIDLLLDRGKEKRSQIVFTRLKTGREVIFWQ
                     TPKTIRTAKPGARIPARRASRVTDLQIVRDTRERYGYRFAKQKATVTRRALPAGDYAV
                     ELDGRIVAAVERKSLDDLVKGLIDGKLIYLMADLALLPRAAVVVEERYSSIFKIDRVK
                     PGFIAESLAAAQVREPRVPIVFCETRPLAEEWTFRFLGAALALETHPANARAAEAAGT
                     YRSDDENE"
     gene            7855..8724
                     /locus_tag="VM841_14245"
     CDS             7855..8724
                     /locus_tag="VM841_14245"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HVL91387.1"
                     /translation="MAGEDSRIVAVCGATGRQGGAVARSVLRRGWHVRALTRKPQRAD
                     ALARLGAEVVRADMNDPASLRAAFDGAHGVYSVQNGMISGFDREVAQGRAVADAAKAC
                     GVAHLVFGSASKGIAGTGVPSWETKVVIEDHIRSLDLPYTMLRPTAFMELMTDKKFYP
                     AVGTWRIWPKLTGETRPIPWLAVDDLGEIAAIAFERPDEFAGKELMLAADLQSLAQCR
                     AIYEETMGKKPPSFPMPQRLFDTFTRNDVTTMWRWLRDGELPVDTATVRSILPSAMSV
                     RDWLARQRDHERG"
     gene            8729..9127
                     /locus_tag="VM841_14250"
     CDS             8729..9127
                     /locus_tag="VM841_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL91388.1"
                     /translation="MAHPNEEHLKRFMDAYAANDADALRGLLADDAVWHVGGTHPLSG
                     DYRGVSEILDYFNNVGAETKGTIALHPIEIMANDTRGATFLRVTASREGRELDVTMAE
                     AFTFDEQGRIREFWAHATDQNAIDEFWRKP"
     gene            9124..9522
                     /locus_tag="VM841_14255"
     CDS             9124..9522
                     /locus_tag="VM841_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVL91389.1"
                     /translation="MMGEKAQKLAEAMGLITQGRTKEFGETLLADDVVWHWPGTSRVS
                     GDYVGRDEALRLLQGFLELTGGRLSVEPIDILEGKDHLMSFTKVTATREGDDLDVTMA
                     DAMRFGPDGRVVEYWTLSNDQNAVDQFIGR"
     gene            complement(9676..10470)
                     /locus_tag="VM841_14260"
     CDS             complement(9676..10470)
                     /locus_tag="VM841_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL91390.1"
                     /translation="MTQFRIGRFTCAVIDAGRVGPSSAADLCLNVPEHEAVDYVGGAE
                     ILAGMNCLMLRDGENVILIDSGLGAFAPDGPERLRAGLDAHGVSPEDVSMVLTSHTHR
                     DHIGGHVMEGAPAFPRARYLFSKVEWDHAAISSAEATQSILPVLADADVVEWLPSGEV
                     LPGIEVIDAPGHTPGHVAFGISDEGMRCLYLADSFLYPRHLERPELYSRYDWDGPMSV
                     ETRKKLIGRAAAERATVVVYHLDAPGLGTIEEADRIYRWVPTIPPA"
     gene            10512..13325
                     /locus_tag="VM841_14265"
     CDS             10512..13325
                     /locus_tag="VM841_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVL91391.1"
                     /translation="MSKPRPDAWAGSIVGRDAETAILKALLNRVPTQGRQLAIIEGEA
                     GIGKSRVLATVLESAREDGFAVLLGVGREFEQDRPFGLLGAALGFTPGRGDPDGSNPR
                     ENRSHIIEAMLDLIEQSCARGPTVLALEDLHWADSSSLLAVGHLVSRLADQPLAVLAT
                     TRPHPRPPELDRVVAELASLGTHVALPPLDAASTVTLARHVLGADPDESLRHSLSSAG
                     GNPLFVIEFAAAMVEDGSVRIEGGTAHSVSLDVPPSLHVTILRRLSFIPTDVLDVLRM
                     ATILGTSFSVTDLATILDRPLAGVTKPLGVAISAGVLGENGERLAFRHDLVRDALYTD
                     QPLAIRKALHLQAGRLLSEAGAPSVQVATQLSMGASPGDADAIRWLRAAARDLGTHGP
                     APAAGFLQRAVELMDPRDPERDAALADLAVSLTWAGKLDEARGHARGVLARTGDAARA
                     AALHYTLAKGWIWHGRPAQSIEELERALALPGLPDGDRALALAELALRLTHARDVSAS
                     FAASREAETLAARTGNRRAAVTAASARAVALEFAGRFREAASVAAEAMRLAETGPPGE
                     SPLVEFDFAQSLIGADRLDEAAAVLESLRRGRIERHQTGMVANVGAVQAGLLLHQGRW
                     DEAVLVGDESVALSADTQFRALLIRVLGVLAVIAVHRDDLARAEELLAEGERDIAISG
                     PGRWGFTWLMWARALLLEARGDLDEALGVLGGAWDLCEMLGSIGERRDIAPDLARLAA
                     AAGDRARLRTIAHGMEQVAAIGGVPSARAAALLCRGVSDEDPAALRAAVAAYRAAPRP
                     VDRAIACEQAAAAVRADAPAEAVEVLQEALGIYEEVGARRDIARVDASLRSAGTRRGA
                     RRPRRRAVSGWESLTPGEREVVKLVAAGLRNTEIATRLYVSRRTVETHLTHVFAKLGI
                     SSRVRLAAEFLRRGEPAAGAERR"
     gene            13541..14575
                     /locus_tag="VM841_14270"
     CDS             13541..14575
                     /locus_tag="VM841_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVL91392.1"
                     /translation="MRRWKAVIAAIGLAAIVPWTAAPAADGYDPLPPPSHWGPGDEAG
                     NSNTQTPAKTLEAVALIETGQKFILGHVYDPTMPMFPGNSWVLEAKPPLPVLQQVANS
                     DFFHGEIGQNGTQLDGLGHFGIHGSLTDPHETLYYNRWKHEEISGPLGLKHLGVETLK
                     PFFTRAVLLDVARHTNGGATLAAGQEITLAMVQQTLAAQGMSESDVRVGDVVLFRTGW
                     EEHWDDGTVAYYAGAPGVPGATPGIGLEVAQWLGTKQVACVGADNWGIEVVPAINPPD
                     GIVYPAHNELIARQGIPLQESMHLQGIADDAAARFENGDENAYVFAYVYVPVPIRGSS
                     GSPGVPMAIR"
     gene            complement(14706..15464)
                     /locus_tag="VM841_14275"
     CDS             complement(14706..15464)
                     /locus_tag="VM841_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91393.1"
                     /translation="MDEQVTIQDATDSATIRGTSDPAASIQARWSSGTAEAQADSTGA
                     FAIKATGLDEGITQVTVSASSPSTEATSATASVNRIFSAGYKKRLADAAAAAKAEAEL
                     QALQGKWSYDSFTDDLSGKEYRIARIQSENTVDFDFPYAGSQHATLMIRQHPRFGLDV
                     FLSIEKGQFLCQSWEDCTVQVRFDNGGIQRWSAVAPEDNSTTVIFLRNASRLLTRLRQ
                     SDTLRIAASFYQEGAPTFIFEVDGFQHSRFLSGE"
     gene            complement(15730..>16213)
                     /locus_tag="VM841_14280"
     CDS             complement(15730..>16213)
                     /locus_tag="VM841_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007813237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HVL91394.1"
                     /translation="GWIDEPIECPICLTPGTRIDTPNGLRAIEAMKPGDPVWTTDADG
                     NRIAGRVVQVVERNVVVPHTLLGVHLRDGRRIRISPGHPLTDGRTIRDLAAGDPLDGS
                     SIIAVSVIRSRHSVTRDLLPSGPTGHYWANGIRMGSTLHHAARPTVRRDALVHSGRLG
                     "
BASE COUNT         2682 a         5517 c         5492 g         2522 t
ORIGIN      
        1 gttcgaggtc ggcgagctgg tgaccgtgcg ctcctatgcg ggtcatcgcg ccgacgaaga
       61 gccgcgcgtc atcgtcgtcg gcggcaagga atatccggtc gagtcgatcg actggcgcgc
      121 catcgaggaa cgcctcgacg gctcgcgccg tcagatcttc gtcgtgcgcg cgggtggcgt
      181 gcgcatccgt ctggcctcca gcgaagaatc ctcacgttgg gaggtcgagc gggtgctcac
      241 ggcgaactaa acatttcggg cggcctcttc atcggcaacc tgaagccgcc gagatcgtta
      301 gggatatcag aacgccgttt cgtcgccgag gtgaccatgt cctggatcga gcgagtgtcc
      361 cgccgccgtg agcggcgcgc cgccgatcgc gccaaggagc gatggaacgg gccgcggacc
      421 gcgttcgttt tgtccggcgg cggcgtgctc ggggcggtcc aggtcggcca gctccgcgcg
      481 ctgatcgaag ccggcatcac gcccgacgtc gtggtcggca cctcggtcgg atccatcaac
      541 ggcgccatga tcgccaccga cccgagcgcc gacgcgatcg acaagctcac cgaaatctgg
      601 accggcctgc gcgccgaaga catctttccc ggaagccgcg tcgctcgcgt ttggaacatc
      661 gtggcgcgcg gcgaccacat ccatcccaac gacggcatcc gccgcctgat cgacatgctc
      721 cccgcgcaca cgttcgaaag cctgtcgctg ccgctgcacg tgtgcgccgc cggcctggcg
      781 gacggcgagg agcgctggtt cgacagcggg ccgctggcgc gcgcgatcct ggcgtccact
      841 gcgctgcccg gcgtttaccc gccggtgtcg atcgacggaa acctttatgt ggacggcggc
      901 gtggtcaaca acattccgat cagccgcgcc gcggcgctag gagcgcgccg tatctatgtg
      961 ctgacgtgcg gggcgcccaa cgcgcgtccc cgcgacgtgc gccgtccgat cgacgttttg
     1021 atccagtcgt tcgcccactc gcgcgccgtg cgcttcgaac tggacaaaga gcgtctgaag
     1081 aacgaggccg agttcgtgat gatgccgacg ttcgacgtcg gagccatccg ctacaacgat
     1141 cccagccact ccaccgagct gatcgaccgc gcctacgaga tgtcggccga attcctcgcc
     1201 cagcccggcg aagccaacgc gtaaacgcgc gtctggccac gcggctcgtt tcacgaagag
     1261 ccgccggacg gcatgatccc tttcgcatcc gggggcgcga agagtacgat cggatccatg
     1321 ccggagcaca ggctgctcac ctacgacgac ctgctgtcgt ttccggacga cggtctgcgc
     1381 cgcgagctca tcgaaggcga gctgatcgtg agcccgtcgc catacactcg gcatcaggtc
     1441 ttatcgggac ggctgtttct cgcgttcgcc aaccatctgg cctcggccgg cggggcgtcc
     1501 gtctagccga aggatacggc aagcccgcga tcctcgagcc cggagagacg ctcagcttcg
     1561 acaagcttcc gggcctggcg atcgactgca ccgcgttatt cacccgttag ccctggctta
     1621 gacaggtcgg cggcacgtag gcttgcaccg ctatggccga tccggcgacg gtggagcgct
     1681 tccgcgagct gcagcggaag ttctcggact tgtggccaac gttgacggtg cgcgcgcagc
     1741 cgattccgcg cacggtcgtg atcgtccatt cgatcaacct cgagctgccg ccgcacctgc
     1801 tgccggtggt gtccgcctat gaagaacgct acctggcgct ggtgctgttg ctgcttcggc
     1861 agccggccgg acgcgtgatc cacgtaacct cgcagccgct gctcccgcag ctcgcgcagt
     1921 actacttcgg gttgatgccg aagctgatcc gtccgagctc gtgggaccga ttcgttcccg
     1981 taagcgtcag cgacggcgga ccgggcccgc tcacgcaaaa gctgctcgac cgtccgctga
     2041 cgatcgagcg gatccgctcg cacattcccg atcccgactg ggcctacatc gtcccgttct
     2101 cggtcggtcc gcgtgagcgc gagctggcgg tgcggctggg cattccgatc tacggcaccg
     2161 acccggacct atcgtggatg ggaaccaaga gcggatcgcg ccgcatgttc gccgaagccg
     2221 gcgtgccgca tcccgcgggc gtcgaggacg tgcattcgat cggcgatgtc gtggaggcca
     2281 tcgcgcagat ccgttcccgc aagcccgacg tcgcggcggt gatggtcaag gtcaacgaag
     2341 ccgcaggcgg gatcggcaac ggggtccttg atctgcgcgg cgccgacgat cgcgccgtgg
     2401 ttgagaagcg cgtgcgcgcg gtcgcgctcg acgaccccga cggcagccgc gacgcgttct
     2461 tcgatcagct cgcgcggacc ggcgggatcg tcgaggagcg gctcgccggc gaagaggtca
     2521 ccagcccgag cgtgcagatc cgcatcgcac ccgacggcgt cgccgaggtc ctcagcacgc
     2581 acgatcagat cctgggcggc gcgaccggtc agatgttcct cggcgcgcgg ttccccgccg
     2641 atcccggcta cgcggccgag atcgcgcggc atgcgctggc gatcgcgtcc cgtctggccg
     2701 atgccggcgc gctcggccgg ttcggcatcg actttgtgtg cacgcgcaac ggcggtggca
     2761 tgtgggacgt caatgcgatc gagatcaacc tgcgcaacgg cggcacgacg catccgttcc
     2821 tgaccttgtc ggcgctgtgt gacggcacgt acgacgccga aaccggaacc ttcaccggct
     2881 cggacggacg gcgaaagttc tacgtcgcct ccgaccatct cgaatccgag gcatacaagc
     2941 gcctgacgcc cgacgacgtg ctcgacacga tcgaagagcg agacctgggc tgggacgtcg
     3001 agcgttgcac cggctatgcg ttccatctca tctcggccgt cgccgtcgcc ggccgcctcg
     3061 gcctgaccgc gatcgccgac tctcccgccg aagcccaaca gatgtcccag cgcgcggaag
     3121 cgaccttcga cgagatctcg gccgcgagcc agccggcacg gtgaggcgac tccgataccg
     3181 ggatcgccgg cccgccgcta cgcggaagcg cgcgtttcgt acattcggat cgtgacgagc
     3241 aggcccgacg ccgccgtgac cgcggcgacg acccagatcg cggcggcgag gccgaacgcg
     3301 tcggcgatca ggcccgacag cagcgcgccg accgcgaacc cggcgtcgcg ccacagccgg
     3361 tagacgccga cggccgacgc gcgccattgc ggatgcgcga cgtcaccgat cgcggcgagc
     3421 agcgtcggat acaccatcgc cgttccgatg cccagcagaa ccgccgcccc cgcccagggc
     3481 gcgaaaccgc gagtcgcggc gaacagcgcc agcgcggccg cttgcacgaa cattccgccg
     3541 gcgatgagcg gcttgcggcc cgtacggtcc gacagcgcgc cggtcaccaa ctggccgagc
     3601 ccccagacgg cgggatacag cgccgcgagt gtgccgatcg ccgcgaccga caacccgccg
     3661 cgcgcgaaga acagcggaaa caagctccat gcgagaccgt cgttgagatt gttgaccaat
     3721 cccgcctggc tgcacgacga caaggcgcgg tcgcgaagcg acgtcaacgc gaagaccttg
     3781 cgggtggaca tgccgccgtc atgcgcgccg gcgtgcgcgg ctgcttcgtg gcgggcgtgc
     3841 ccgtgggttt cgcgaacgaa gacggtggac aggcccagcc cgagcgccgc ataggacagt
     3901 cccagcagga acggccccgg gcgcagtccg tagcgcgagg cgatcaagcc ggttgcgact
     3961 gcggtgacgg cgaccgcgat gtagccggcg gcttcgttca atcccatcgc cagtccgcgt
     4021 cgcgccggcc cgaccagatc gatcttcatg atcaccgtcg tggaccaggt gagcccctgg
     4081 ttgacgccta gcagcgcatt cgccgccacg acccaccacc agttcggcgc aaggatcagc
     4141 atgatcggaa cgggcagccc gatgatccag ccggcgacca gcacgggttt gcggccgatc
     4201 cggtccgcca acgctccggc gacgaagttg gtcagcgcct tgacgatccc gaacgcgatt
     4261 acgaacgtcg cgacggcggt gaacgcgcga acgttgaaga cgcgatcggc gagcagcggc
     4321 agcacggtgc gctcttgacc gagcatgccc ccgacgagcg cgttgacccc gacgagcagc
     4381 ccgaactgcg cggcgttctg gcgcagcccc agccggaccg gcgcgtcgat catgcgccac
     4441 gttccagcgg catgccggtc gtgcgatgcc agtcgccggg gccggcggcc agcacgcgca
     4501 cgccgcggtg gccgttgcgc tcgagcacgc tcgcggcggt cgtcgcgcgt tcgccgtgac
     4561 cgcacatgac cgtcagcgat ggcggaaggg tgggcgcgtc cgcgagcgcc cccagctcaa
     4621 cgtgcagcgc gccgggcacg tggccggccg tgaactcgct gtcttggcgg acgtcgagca
     4681 gcggtccgtc cacgaccggg tcggtgacga cctcgacgcg ggacagcgcc cgtcccgcgt
     4741 cccgccacgc gtcgatgccg ccgtcgatct cgccgatcag gttgtcgtag ccgatcgagc
     4801 ggcatgcgcg gatcagctcg ccccgcgact gtgcatcatc gatcacgaac agcaggggcc
     4861 ggtcgcgatc gaccagccag cccagccagc tggcaaaagc gggacgcagc tcgatcgaga
     4921 gcgaaccggg gatgtgcgcg tcggcccacg cgcgcgcgga tcgcacgtcg atcagctcgg
     4981 ctccgccccg cattgccgcc tcggcgtcgt ccggcgcgag ccgatcgagc gacaggggcc
     5041 cgtccgacga cgctccccgg cggttcacct cgcgcagccg aaggaagtaa gggggatagc
     5101 tgcccaggcc ggcgagcagg ccggcgacga agtcgtcctc gctttgggtg aagaccggat
     5161 tggctgcccg ttcgcgtccg atcgtcgtgg tgcgttcgcc ggtggcggcg accgagcaga
     5221 acgatccgcc gccgtgcgtc ggatacacga tcacatcgtc gggaagcggc gcgatgcgct
     5281 cgtggacggc ccggtgcagc cgtcgcgcga gcggttcggt ttcctcgggc gcgatcaggt
     5341 cggtgcgcgc gacggttccc ggcagcagcg ccccgccgct cagcagcccc accggccgcg
     5401 cgtcgcgcag gatcagatac gacagatgct cgggcgtatg gccgggtgtt tcgatcgcgc
     5461 gcacgatcgc gtcgcccagc ggcagttcgt ctccgtcggc cagcggccga tgcggaaatg
     5521 cgggagcagc ggcggcgggc accgcgacag tgatgccatc cgcggcgagc gcgcctgctc
     5581 ccgagacgaa gtcggcatgc aggtgggtct cgatcgcgcc ggccagcttg agcccgcgct
     5641 ctcgcgcgcc gcgccggtag ggttccaggt cgcggaccgg atccaccacg atcgccgatc
     5701 cgtcgccgcc gtcgatcagg tacgaagagt tgcccaaccc ctcgtcggga aaggcgacga
     5761 tctcgatcac ggcgacaccc cgcgtcctgc gctcgcgacg ggcaagcccg cgtcgcgcca
     5821 ctcggggaag ccgtcgatca atcgatcgtt cgcgcctcgg tctcccatct ctcttcaaac
     5881 gctaccatcg ccttctccaa tgttcaaacg aacgcttgat ttcagagggc cgaggccgta
     5941 cgatgccggc agccgaagaa gaaggaggct ctatgtacgg gaccgtgttt catttccagg
     6001 ccaagccgga tgcggccgac gatgtcaagg ccctgttcgc cgaatggaat cgtgacgtga
     6061 agccgacggt cgacggggcg ctgaatggat tcctctacca gctcgattca gacccggatc
     6121 gtttcatcgc gtgtgcggtc ttccaggaca aggcgtccta catgaagaac gcgaacagcc
     6181 ccgagcagga caagtggtat cagcgattcc gtgatctgct caccggcgac cccgactgga
     6241 acgacggcca gatctcgtag cccagcggag gcagttcccg accctcgacc gtgacgcaag
     6301 cgaaacgggt tggagcaccc cgcgtcgcga tccgggcggc atggagccgc ccggacgttg
     6361 tcccctccac accggggaat ccaggcgact cgccacacat ccactcgcag tcactcgagc
     6421 cggcgctcag gtgacagggt ggcctatcgc ctcgttcgac gcgaataacc ccaaccggcg
     6481 ccggagagaa tcatgacggc caacacgatc aaaagtacgg taagcatctg agaacacctc
     6541 ctgtctcgat gccattgttc tcgtcgtgcg ttaccggccc gtcaccgcgt atttcacgcg
     6601 ggccgtgctg ctcgacctag cgcgacacgt gatcggcctc acccgcctag cgatcgcgaa
     6661 gatctcgtag aatctctcct cgatggcgcc gacgttgctg atcgcgcgga acccggatcc
     6721 ggactcgtcg ctgccctaca tcatgcgcgt gccgatcggc ggcggcctgg tgctgctggt
     6781 ccgcgacacc tggccgcgca cggccgcggt ttactgccac ccggcgcccg agtggccggc
     6841 cggcgcggag atcgtcgaag agattccggt gcgctcgtgc acccggcgcg gaccggcgat
     6901 cgacttgctg ctcgaccgcg gcaaagagaa gcggtcccag atcgtgttca cgcgcctcaa
     6961 gaccgggcgc gaagtgatct tctggcaaac cccgaagacg atccgcaccg cgaagccggg
     7021 cgctcgcatt ccggcgaggc gcgcgtccag ggtgaccgat ctgcagattg ttcgcgacac
     7081 gcgcgagcgc tacggctacc ggttcgccaa gcagaaggcg acggtgacgc gccgcgcgct
     7141 gccggccggc gactacgcgg ttgagctgga cgggcgtatc gtcgccgccg tcgagcgcaa
     7201 gtccctcgac gatctggtca agggattgat cgacggcaag ctcatctacc tcatggccga
     7261 tctcgcgctt ctgccgcgag ccgccgtcgt cgtcgaggag cgctattcgt cgatcttcaa
     7321 gatcgaccgc gtcaagcccg gcttcatcgc cgagtcgctc gccgccgcgc aggtccgcga
     7381 gccgcgcgtg ccgatcgtgt tctgcgagac caggcccctg gccgaagagt ggaccttccg
     7441 attcctcggc gccgccctcg cgctcgaaac ccaccctgcg aacgcccgcg ccgccgaggc
     7501 cgcgggaacc tatcgctcgg acgacgagaa cgagtagagg cacgccagtt ccatctggcc
     7561 tgggcaggac ctccgtaggt ccttgcacac cctgtgcaac taagttgcaa aggagaccgg
     7621 ctcccgccga gcccgtcgcc cattgaacat gtgcaacaca tgtgacatgt tgcgtgcata
     7681 ttcagatgat ccaggtgcgc aacgtgcccg accggctgca tcgcgacgct cgtacgcgcc
     7741 gagcggcgcg gccgggaggc gctcggacca ggcaccgcga ctcccggaga cgctcgcacc
     7801 ggtttgggtg gtgccttgac atcgaagcgg cgcgcgggag aggattcccg agccatggca
     7861 ggcgaggact cccgtattgt cgccgtctgc ggcgccaccg gccgccaggg tggtgccgtt
     7921 gcgcgcagcg tgctgcgtcg cggatggcat gtgcgcgcgc ttacgcgaaa gccgcaacgt
     7981 gcggatgcgc tcgcgcggct cggcgccgag gtggtgcgcg ccgacatgaa cgatcccgcc
     8041 tcgctgcgcg cggccttcga cggcgcgcac ggcgtctaca gcgtgcaaaa cggcatgatc
     8101 agcggcttcg atcgcgaggt cgcccagggt cgcgcggtcg cggacgcggc gaaagcctgc
     8161 ggcgtggcgc acctcgtctt tggctcggcg tcgaagggta tcgccggcac gggcgtgccg
     8221 tcgtgggaga cgaaagtcgt catcgaagat cacatccggt cgctcgactt gccgtacacg
     8281 atgctgcgtc cgacggcgtt catggagctg atgaccgaca agaagttcta tccggccgtc
     8341 ggcacgtggc ggatctggcc gaagctgacc ggcgagacac gcccgatccc gtggctcgcg
     8401 gtcgatgatc tgggcgagat cgccgcgatc gccttcgagc gacccgacga gttcgccggc
     8461 aaagagctga tgctcgccgc ggatctgcaa tcgctcgcgc agtgccgcgc gatctacgag
     8521 gagacgatgg gcaagaagcc tccgtcgttt ccgatgccgc aacgattgtt cgacacgttc
     8581 actcgcaacg acgtcaccac gatgtggcgg tggctccgcg acggcgagct gccggtcgat
     8641 accgcgaccg tgcggtcgat cttgccgtcg gcgatgtcgg tgcgcgattg gctcgcgcga
     8701 cagcgcgacc acgagagggg gtaacggcgt ggcccatccc aacgaggagc acctcaagcg
     8761 cttcatggac gcctacgcgg ccaacgacgc cgacgcgctt cgtggcttgc tcgccgacga
     8821 cgcggtctgg cacgtgggcg gaacgcatcc gctcagcggc gactatcgcg gcgtgagcga
     8881 gatcctggat tacttcaaca acgtcggcgc cgagacgaag ggcacgatcg cgctgcatcc
     8941 gatcgagatc atggcgaacg acacgcgcgg cgcgacgttc cttcgcgtca ccgcctcacg
     9001 cgaagggcgc gagctggacg tgacgatggc cgaggcgttc acgttcgacg agcagggacg
     9061 catccgcgag ttctgggcgc acgcgaccga ccagaacgcg atcgacgaat tctggaggaa
     9121 gccatgatgg gcgagaaggc gcagaagctt gccgaggcga tgggactgat cacccaagga
     9181 aggaccaaag agttcggcga gacgctcttg gccgacgatg tcgtttggca ttggcccggc
     9241 accagccgcg tcagcggcga ttacgtcggg cgcgacgagg cgctccggct gctgcaaggc
     9301 ttcctcgagc tgaccggcgg gcggctcagc gtcgagccga tcgacatcct cgaaggcaaa
     9361 gatcatctga tgtcgttcac gaaagtgacc gcgacccggg aaggcgacga cctggacgtg
     9421 accatggccg acgcgatgcg tttcggaccg gacggccgcg tggtcgagta ctggaccttg
     9481 tccaacgacc agaacgccgt cgatcagttc atcggccggt agaggcagcc gcgacgtccg
     9541 agtgcgtggc ggcagtcgtt cgcgccgaag gatacggcct gggcgaattc ttgctgcgct
     9601 atccacacgc atacgtcgcc ctctcgagca tacgcatcga gaaatcgtga cggccgtcga
     9661 cgaagactcg ccccgttacg cgggaggtat cgtcggcacc catcggtaga tgcggtctgc
     9721 ttcctcgatc gtgccgagcc ccggtgcgtc caagtggtac acgacgaccg tcgcccgctc
     9781 ggcggcggca cggccgatga gcttcttgcg agtctcgacc gacatcgggc cgtcccagtc
     9841 gtagcgcgaa tacagctcgg gccgctccag atgccgcgga tagagaaacg agtcggccag
     9901 atagagacat cgcatgccct catcgctgat gccgaaggcg acatgccccg gggtgtgccc
     9961 cggcgcgtcg atcacctcga tgccgggtag cacctcaccc gacggcagcc actcgacgac
    10021 gtcggcgtcc gccaacaccg gaaggatcga ctgtgtcgct tcagcggagg atatggccgc
    10081 gtgatcccat tcgactttcg aaaacaggta ccgcgcgcgc ggaaacgccg gggccccttc
    10141 catcacgtgt ccgccgatgt gatcgcggtg cgtgtggctg gtcaacacca tcgagacgtc
    10201 ctcgggtgac acgccgtgcg cgtcaaggcc ggcgcgcaac cgctcggggc cgtcgggcgc
    10261 gaacgcgccc aagccgctgt cgatgagaat gacattctca ccgtcgcgca gcatgaggca
    10321 gttcattccc gccaagattt cggcgccgcc gacgtagtcg acggcctcgt gctccggaac
    10381 attgagacac agatccgccg ccgacgacgg cccgacgcgg cccgcatcga tgaccgcgca
    10441 cgtgaaccgc ccgatacgga actgcgtcat ccagatcgcc ccttggcagc gaagcgtatc
    10501 atcgcgggag gatgtccaaa ccccgtcccg acgcctgggc cggcagcatc gtcggccgag
    10561 acgccgaaac cgccattctt aaggctttgc taaatcgcgt gcctacgcag gggcggcagc
    10621 tcgcgatcat cgaaggcgaa gcgggcatcg gcaagagccg cgtgctcgcg acggtcctcg
    10681 aatccgcgcg agaagacgga ttcgcggtcc tgctcggcgt gggccgcgag ttcgagcagg
    10741 atcgcccctt cgggctcctc ggcgccgcgc tgggcttcac gccgggacgc ggcgaccccg
    10801 atggcagcaa tcctcgcgag aatcgctccc acatcatcga ggcaatgctc gatctcatcg
    10861 aacagtcgtg cgcgcgcggc ccgacggttc tcgcactcga agatctccac tgggcggact
    10921 cctcgtcgct gctcgcggtc ggtcacctgg tgtcgcgtct ggccgaccag ccgctggccg
    10981 tgctcgcaac gacgcgtcct cacccgcgcc cgcccgaact cgaccgcgtc gtcgcggaac
    11041 tggcctcgct cggcacgcac gtggcgctgc caccgctgga cgcggcctcg acggtcactc
    11101 tcgcacgaca tgtgctcggc gcggaccccg acgagagcct tcgccacagc ctgtcaagcg
    11161 cgggcggcaa cccgctgttc gtcatcgagt ttgcagcagc gatggtggaa gacggatccg
    11221 ttcgcatcga aggcggcacc gcgcacagcg tctcgctcga cgtgccgccg tcgctgcacg
    11281 tgaccatcct gcggcggctc agcttcatcc cgaccgacgt gctcgacgtg ctccgcatgg
    11341 cgacgatcct cggcacgagc ttctcggtga cggatctcgc gacgatcctc gaccggccgc
    11401 tcgcgggtgt gacgaagccg ctcggcgtcg cgatcagcgc cggcgtcctc ggtgagaacg
    11461 gcgagcggct cgcgttccgc cacgacctgg tccgcgacgc cctctacacc gaccagccgc
    11521 ttgcgatccg caaggctctg cacctgcaag ccggccggct tctgagcgag gccggcgcgc
    11581 cgagcgttca ggtggcgacg cagctttcga tgggcgcctc gcccggcgac gcggacgcga
    11641 tccggtggct gcgcgccgct gcgcgcgacc tggggacgca cggaccggcg cccgccgccg
    11701 gatttttgca gcgcgcggtc gagctgatgg atccgcgtga tcccgagcgc gacgcggcgc
    11761 tcgcggacct tgccgtctcg ttgacgtggg cgggcaagct ggacgaggcg cgcggtcacg
    11821 cgcgcggcgt gctcgcgaga accggcgatg cggcgcgcgc ggcggcgttg cactacaccc
    11881 ttgcgaaagg atggatctgg cacggacgcc ctgcgcagtc gatcgaggag ctcgaaaggg
    11941 cgctcgcgtt gccgggcctt cccgacggcg atcgcgcgct cgcgctcgcg gagctggcgc
    12001 tgcgcctgac gcatgcgcgg gatgtgtccg cctcgttcgc ggcttcgcgc gaggccgaga
    12061 cgctcgccgc gcgaaccggc aaccggcgcg cagcggtcac cgccgcgtcc gcgcgcgccg
    12121 tcgcgctcga gttcgccggc cgcttccgcg aggcggcgag cgtcgccgcg gaggcgatgc
    12181 gactcgcgga gaccggcccg ccgggcgagt cgccgctcgt cgagttcgat ttcgcgcaaa
    12241 gcctgatcgg cgcggaccgc ctcgacgagg cggctgcggt gctcgagtcg ctccggcgcg
    12301 gccggatcga gcggcatcag acggggatgg tcgcgaacgt cggagcggtt caggcgggcc
    12361 tgctccttca tcagggacgc tgggacgagg cggtcttggt cggcgacgag agcgtcgcgc
    12421 tctccgccga tacgcagttc cgagcgctgc tcattcgggt gctcggcgtg ctcgcggtga
    12481 tcgcggttca tcgcgacgac ctcgcgcgcg ccgaggagct gctcgccgaa ggcgagcggg
    12541 acatcgcgat atcggggccc ggccggtggg gtttcacgtg gctgatgtgg gcgcgcgcgc
    12601 tgctgctcga ggcgcgcggt gatctcgacg aagcgctcgg agtcctcggc ggcgcatggg
    12661 atctgtgcga gatgctcggt tcgatcggcg agcggcgcga catcgcgccg gacctcgcgc
    12721 gtctcgcggc ggcggcgggc gatcgcgcgc ggctccgcac gatcgcgcac ggcatggagc
    12781 aggtcgccgc gatcggcggc gtgccgtcgg cgcgtgcggc ggcgctgctg tgtcgcggcg
    12841 tgagcgacga ggatccggcc gcgttgcgcg ccgcggtcgc ggcctatcgc gcggcacccc
    12901 ggccggtgga tcgagcgatc gcgtgcgagc aggcggcggc agccgttcgc gcggacgctc
    12961 ccgcggaggc cgtcgaggtg ctgcaggaag cgctcggcat ctacgaagag gtcggcgcgc
    13021 gccgcgacat cgctcgcgtg gacgcgtcgc tgcggtcggc gggaacgcga cgcggcgcgc
    13081 ggcggccgcg gcgtcgtgcg gtcagcgggt gggagagcct cacgccgggt gagcgcgagg
    13141 tcgtcaagct cgtcgcggcc gggttgcgca acaccgagat cgccacgcgg ctctacgtgt
    13201 cacgccggac cgtcgagacc catctcacgc acgtcttcgc caagctcggg atctcctcgc
    13261 gcgtccggct ggccgcggag ttcctccggc gcggtgagcc cgctgccggc gcagagcgac
    13321 gatagcgcgc cgagcagccc cagccagccg gtgaacgcga cgtcatcctt tcccgggatg
    13381 ccggcacgcc ccgtcggcgg gtcggaccca tcgaagaaga tgcgcacgat catgcggtca
    13441 tcatcggcca tcgcgatccg cgcaaacatc gtggacatcc acgatgtttc ggccggccgg
    13501 gccccctagg gtcgaaccgt ccacgactga agggggagtc atgcgcagat ggaaggcagt
    13561 catcgcggcg atcggcctgg ccgcaatcgt gccctggacc gcagcaccgg ccgctgacgg
    13621 ctacgacccg ttgccgccgc cctcgcactg gggacccggc gacgaggcgg gcaactccaa
    13681 cactcagacg ccggccaaga cgctcgaggc ggtggcgctg atcgagaccg gccagaagtt
    13741 catcctgggt cacgtctacg accccaccat gccgatgttc ccgggcaact cgtgggtgct
    13801 cgaggccaag ccgccgctgc cggtccttca gcaggttgcc aacagcgatt tcttccacgg
    13861 tgagatcggc cagaacggca cgcagctcga cgggctgggg cacttcggca ttcacgggtc
    13921 gctgacggat ccgcacgaga cgctctacta caaccgctgg aagcacgaag agatctccgg
    13981 accgttgggt ttgaagcatc tgggcgtcga gacgctcaag ccgttcttca cgcgcgcggt
    14041 gctgctcgac gtcgcgcggc acacgaacgg gggcgcgacg ctggcggccg ggcaggagat
    14101 cacgctcgcc atggtccagc agacgctggc cgcgcaaggg atgagcgaat ccgacgtccg
    14161 cgtcggcgac gtcgtgctgt tccgcacggg gtgggaggag cactgggacg acggaaccgt
    14221 ggcgtactac gccggcgcgc cgggcgtgcc cggcgccacg ccgggaatcg gcctcgaggt
    14281 tgcgcagtgg cttgggacga agcaggtcgc ctgcgtcgga gccgacaact ggggcatcga
    14341 ggtcgtgccc gcgatcaacc cgcccgatgg catcgtctat cccgcgcaca acgagttgat
    14401 cgcgcgccaa ggcattccgc tgcaggaatc gatgcacctg cagggaatcg cggatgacgc
    14461 cgcggcccgc ttcgagaacg gggacgagaa cgcgtacgtg ttcgcctacg tgtacgtgcc
    14521 ggtcccgatt cggggatcgt cgggatcacc gggcgtgccg atggcgatcc ggtagcgcga
    14581 gagtttttcg gtgcggtcgc gaccgagcct tcgtggtccg gtcgcgaccg cactcgggaa
    14641 caattcgacg agcggaagca acgtgccggt taccgtctcc gggtgtcccg caactcttgg
    14701 aacaatcatt ccccgctcag gaagcgcgaa tgctggaaac cgtctacctc gaaaatgaag
    14761 gtcggggcac cctcctgata gaagctggct gcaatgcgca gagtatcgga ttgccgcaat
    14821 cgcgtaagca gccgggaggc gttgcgcaag aatatgactg tcgtggagtt atcctccggc
    14881 gcgacggccg accagcgctg aataccgcca ttgtcgaaac gcacctggac agtgcagtct
    14941 tcccaggact ggcaaaggaa ttggcctttc tcaatggaaa gaaacacatc gaggccaaag
    15001 cgaggatgct ggcgaatcat caacgttgca tgctggctac cagcataagg gaagtcgaaa
    15061 tcaacggtgt tctcggattg gatgcgcgct attcgatact ccttccccga caggtcgtcg
    15121 gtgaaagagt cataggacca tttcccctgt agggcctgca attctgcctc tgccttcgca
    15181 gctgcggcag catccgcaag cctcttcttg tagccggccg agaagattcg gttcacactt
    15241 gcagtagctg atgttgcctc ggtcgaaggc gaggacgctg aaacggtgac ctgggttatc
    15301 ccttcgtcaa gcccggtggc cttaatcgcg aacgcgcccg tggagtcggc ttgggcttca
    15361 gccgttccgg acgaccaacg tgcctgaata gatgcggctg gatcgctcgt gccacgaatg
    15421 gtggccgagt ccgtcgcatc ctgaatcgtt acctgctcat ccaccagccg cagagcggtc
    15481 tggcgaagtg gcgagggggc agctttggaa gctttccccg acgaacccat cgtggctttc
    15541 tgagccaggt tggtcgtcaa gagaagggct cccgcggcca acgcgatgat caggcctttg
    15601 cgggcacccc tggcccgctt aaaatgtggt gcaacttcga cctgatgatc ctcttcgcta
    15661 tggtgggacc tgctaagcaa catctgacac accccctcga aagatgatcg accgccacga
    15721 ttgacgtctt tagcccaacc tgcctgaatg cacgagtgcg tcgcggcgca ccgtcggtcg
    15781 ggcggcgtgg tgcagggtgc tgcccatgcg gattccgttc gcccaatagt ggccggtggg
    15841 gccggacggg aggagatctc gcgtaaccga atgccgcgag cggatcaccg aaacggcgat
    15901 gatcgacgag ccgtccagcg gatcgcccgc ggcgaggtcg cggatcgttc gcccgtcggt
    15961 cagcgggtgg cccggtgaga tgcgaatccg acgcccgtcg cgaaggtgta ctcccagaag
    16021 cgtgtgggga acgacgacgt tccgctcgac gacctgcacc acgcggcctg cgatccggtt
    16081 gccgtcggcg tccgtcgtcc acaccgggtc gccgggcttc atcgcctcga tcgcgcggag
    16141 gccgttcggc gtgtcgatac gcgttcccgg cgtgaggcag atcgggcatt cgatcggctc
    16201 atcgatccac cca
//
LOCUS       DATLFO010000352        11186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_146692, whole genome shotgun sequence.
ACCESSION   DATLFO010000352 DATLFO010000000
VERSION     DATLFO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146692"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VM841_14285"
     CDS             complement(<1..407)
                     /locus_tag="VM841_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HVL91395.1"
                     /translation="MLEFRPRELAALAALSLIVAGGAVFAFARTMPRPGEPAAAAATR
                     SLAPIETLAPSPSSPATLFVHVAGAVRAPGVYEFAAGARVADALRAAGGATGEADANS
                     INLARPLSDGERIYVPRRGETPPPDPGGAGGGGG"
     gene            complement(539..1570)
                     /locus_tag="VM841_14290"
     CDS             complement(539..1570)
                     /locus_tag="VM841_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVL91396.1"
                     /translation="MGAGHAHPAALTGAEGTRAGLRAIWIAAIALGASAVVRLVIAYI
                     SGSVGLLAAGLDDLGDVLTTIALSIAFVASRRAADRRYTFGYQRFEDLSGVLVVLVIW
                     SSAAYATFEAVRKLGGGHEVQQIGIAITAAAAGLIANGFAGFYKIGVGKRIGSQPLIA
                     DGKHALTDGVASIAAVAGLLGVQAGAIWADPVAGLVVVLAIVWVAIQATRNVLRQLLD
                     AVDPVLIDRIEEVARREPGVEAVGRIQARWAGRSLYVSLTVAVDEHMTVANAHDVAEH
                     LHHEILHAIPGVAQVDVHVDPGEPHGPGAHEHTGAHAARERGEHVHSTHHDDGRHLGQ
                     DHPQDHHDH"
     gene            complement(1576..4044)
                     /gene="leuS"
                     /locus_tag="VM841_14295"
     CDS             complement(1576..4044)
                     /gene="leuS"
                     /locus_tag="VM841_14295"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HVL91397.1"
                     /translation="MTDEDRYDPSSIEPKWVDEWERTEPYRASDDPNDPRPRFYALDM
                     FPYPSGDLHMGHLEALGGGDVIARYWMLRGYNVLHPIGWDAFGLPAENAAIKRGANPR
                     SWTYENIEQQAKSFKRLGMSFDWSRRFNTCDPGFYTWTQWLFLKLREAGVAYRSSAPV
                     NWCPNDKTVLANEQVVQGRCERCDAAVVKRDVTSWFFRESAYAQRLLDDMDQLTGWSE
                     HVKIIQRNWIGRSEGADVTFAIAETGEQITIFTTRPDTLWGVTFFVIAPEHPMARVLA
                     ERAGKAGAFEAYLREVGAASEIERTALGRRKLGFDTGAHAVNPVNGEQVPVYVADYVL
                     MEYGTGAIMAVPAHDQRDFEFARQEGLPVRVVIQPEGGALDGQTMEAAYDGEGRMSSS
                     GHFDGSPTPESIAKVIAWLEEQQLGTGRVRYRLRDWNVSRQRFWGCPIPIVYCEQCGD
                     VAVPDDQLPVLLPDLEDYTPTEEGESPLAKVTDWVNTSCPQCGSAGKRDIDTFDTFVD
                     SSWYFFRYCSPHTGDAPFDRELVERWLPANQYIGGDTHATGHLIYSRFFTKALYDLGM
                     ISFTEPYPNLFNQGTVIMEGAKMSKSRGNLVTPSAIVDEFGADTARVTMLFAGPYEAD
                     IDWADVSPHGVFRWLSRVWRLAVENAGRIVAAGAPSGEGELRRRTHRAIQGAGHDTER
                     FRFNTAISKLMVLTNDIAELAGSSDDADVAEAVRALVVMLNPYAPFITEEVWHRLGGQ
                     GHLIHARWPEAIDALAARDSLTMVVQVNGRVRDKIEVVPDISGDAMEALARASDKVAA
                     FVEGKTVVKTIVVPPKLVNIVVRD"
     gene            4168..6108
                     /locus_tag="VM841_14300"
     CDS             4168..6108
                     /locus_tag="VM841_14300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVL91398.1"
                     /translation="MKPEHVYDLTGATDPRLSPDGRTVACTVWRVDRDANAYRGAIWL
                     TATDGSEPPRRLTSGEHRDGSPRWSPDGTMLAFVRTRGDVKDAEAQLCVLPLAGGEPR
                     TLTDLKESVEDPSWSPDGSTLLFVARTHDPDDDERDERRRAPRRITRLQFKLDSVGWT
                     AGRRQHLYTVPADGSAPATRITDGDFEDADPCWSPDGTQIAFCSARDEDWDTTMARDL
                     FVMPANGGEPRKLTASDGAASSPSWSPDGRRIACLWYPVVFDDPRHTQVAVFDAASGE
                     RTVLTDSLDRNCGIYPTLREPIWDGEDLIFATEDSGNNPVYRVRADGSGKPEPILEGD
                     FMLTGLDAAAGVVVHAATTAVTPSEIFNGATRLTSVGDALVAAIRPQTPERFTAISAD
                     GSEVEAWIIPPANAEPGARYPTLLNIHGGPFSQYGNKLFDEFQVQAGAGYAVVYANPR
                     GSSGYSEAWGRAIRPADGSGWGGVDFQDLMAVMDEAERRFNFVDPSRTGVLGGSYGGY
                     MTSWIVAHTDRFKAACSERAVNNFVMEGGSADLGWMFQGEFGTTWYEDPHAYLKMSPS
                     MYASSITTPLLILHSESDLRCPVGHAEDLFTILRLRGRPVEMVRFPAESHELSRAGSP
                     VHRVQRFEIILEWFDRHLKDPA"
     gene            6105..6806
                     /locus_tag="VM841_14305"
     CDS             6105..6806
                     /locus_tag="VM841_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVL91399.1"
                     /translation="MTDEVLVESDGPVRVVTINRPAKRNALHIQLRRALATAFARLGT
                     DPDVGVVVLAGAGSAFCSGMDTTQFGGDDDNRRALFESSYGLFEALRTLPKPVIAAVN
                     GPALAAGFALAAFADIRIAGPAATFGMPEVSLGFASSWATLARVLPDQLARRMAFTGE
                     TIDAATALEWGLVMEVAPDPLARARALAGVMAANPVPGLEGTKRLMVESADPALRAAW
                     ETEMRIFHTSLFRKR"
     gene            6876..7379
                     /locus_tag="VM841_14310"
     CDS             6876..7379
                     /locus_tag="VM841_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91400.1"
                     /translation="MKKLLAAVAVAAMLAPGSAGAATDVAAVGQGNGTITRTNGTCSA
                     PLALTFNVRSNVAAVTESWGPGGPNTESCALATILRLYPTTTAPVPAVQQCLPGISIH
                     SGGAWTLSQSGQTYTMQRTYRLCNGKQIFDKIVVTVQATRLVYSNTYAENGVTLVTVT
                     ANVPRIA"
     gene            7390..7824
                     /locus_tag="VM841_14315"
     CDS             7390..7824
                     /locus_tag="VM841_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91401.1"
                     /translation="MKRLALVLTALALLGPSSSPAASDATAAGHGNGSATYAGVTCNA
                     PVAITFVVRASAAVISDTWGAGTTPAACTPVGGVMVAGSSALSQSGSTYTAQSSYTGT
                     DGAPMTDKVVVTVQSNRLVYSRTVTRSGIVVESASANVPRVA"
     gene            7950..8456
                     /locus_tag="VM841_14320"
     CDS             7950..8456
                     /locus_tag="VM841_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91402.1"
                     /translation="MKRLLLALAAVALLVPAAAPAATDVAAVGQGNGTIKVNNLTCAA
                     PLAVTLNVRSNVGVVTENWGPGNPSPLLDCIFMPVFGQYEADNTPVPPVQQCVQGAGL
                     STGMPYVLSQSGSTYTFTGSTRLCNGKQVLDRVSITVQTTRLVYSHTWSENGVAIVTV
                     NANIPRLA"
     gene            8727..9236
                     /locus_tag="VM841_14325"
     CDS             8727..9236
                     /locus_tag="VM841_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91403.1"
                     /translation="MQIADVQIREAAREDESALTELAYEYLSWAIERLRSDRGVEWPA
                     ITRDDVAEAIDSYRNGGAILIATVDAAPVGMGALRRLDEGAFEIKRMYVRPAARGHRV
                     GARILDDLLLRARAAEGRVVRLDTIVFMDDAQRLYRSRGFVQRGPYEESEIPTRLQAH
                     WLFFELALR"
     gene            complement(9387..9459)
                     /locus_tag="VM841_14330"
     tRNA            complement(9387..9459)
                     /locus_tag="VM841_14330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9424..9426),aa:Ala,seq:ggc)
     gene            9610..9798
                     /locus_tag="VM841_14335"
     CDS             9610..9798
                     /locus_tag="VM841_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91404.1"
                     /translation="MLWALSRLETFFVEPVEHAERISKLMRQADTEGLEAEVARLLAP
                     SVEVATRRSARRRSLAAT"
     gene            complement(9858..11153)
                     /locus_tag="VM841_14340"
     CDS             complement(9858..11153)
                     /locus_tag="VM841_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91405.1"
                     /translation="MRRRVSLLAAVVAILASAMTSGAEPLPPLAADLLDVEYVTGDNG
                     LPGGHIAIEGDRLYLGSYGLGMRIYDIKDPSAPALIGQYMPGARFDAVPDAAVWEGRH
                     IAVLNGTKRAERTDRAEFLDVTDAANPKLLWTFEGPDHGESHNGDIVDKRKLWLPSGA
                     KGRGGLRIYDMRPLLLPEPKAPDEVFAGNPVTMWRASPYRRGRDVGPAFTHTHDITVY
                     TDYPVLVPRREWIDLNRDGKPDPTFRGKDIALLAEGGDYLNDAGNTGSIFVIDITDPS
                     RPVVMLRWIHETGVGHHPIRYNHEAQLLDGDKHVMLVTDEDMHSGCGVSGGITAVRLW
                     ANMTDAHELSEWKVPAGAAEGICSTHVFSSKGKTVYMGAYNAGLQVIDYSDPRAPKVI
                     GSYVAEGATAWGAYEHKGYVFMGDMTRGLDVLRPIRRKK"
BASE COUNT         1718 a         3914 c         3813 g         1741 t
ORIGIN      
        1 cccccgccgc caccggctcc accggggtcg ggcggcggcg tttcgccgcg gcgaggcacg
       61 tagatgcgct caccgtcgga gagcggcctg gccaggttga tcgagttcgc gtcggcttcg
      121 ccggtcgcgc cgcctgcggc ccgcagggcg tcggcgacgc gcgcgccggc ggcgaactcg
      181 tacaccccag gggcgcgcac cgcgcctgcg acgtggacga acagcgtcgc cggcgacgac
      241 ggcgagggag cgagcgtttc gatcggcgcg agcgagcggg tcgcggccgc ggccgcgggc
      301 tcgcccggcc gcggcatcgt gcgcgcgaac gcgaagaccg ctccgcctgc cacgatcagc
      361 gacagcgccg cgagcgcggc cagctcgcgc ggccggaatt cgagcatgcg ggcaagccgc
      421 tctctgaggc cgagcggctc gagggccggc gcgtcggatg ccacgggtac cggccgacgc
      481 tacgaagccg gatgcggccg gtcccgtccg ggaagacccg gcagaacccc tcgcgccgtc
      541 agtgatcgtg gtgatcctgc gggtggtcct ggccgaggtg acgaccgtcg tcgtgatggg
      601 tgctgtgcac gtgctcgccg cgctcacggg ccgcgtgggc gccggtgtgc tcgtgcgcgc
      661 cgggcccgtg cggctcgccg ggatcgacgt gcacgtccac ctgtgcgacg ccggggatcg
      721 cgtgcaggat ctcgtgatgc agatgctcgg cgacgtcgtg ggcgttcgcg accgtcatgt
      781 gctcgtccac cgcaacggtc agcgacacgt atagggagcg gccggcccag cgcgcctgga
      841 tgcggccgac ggcctcgacg cccggctcgc gccgcgcgac ctcctcgatc cggtcgatca
      901 gcaccgggtc cacggcgtcg agcagttggc gcagcacgtt gcgggtcgcc tggatcgcga
      961 cccacacgat cgcgagcacc acgaccaggc cggccaccgg gtccgcccag atcgcgccgg
     1021 cctgcacgcc gagcagcccg gcgaccgcgg cgatcgaagc cacgccgtcg gtgagcgcgt
     1081 gcttgccgtc ggcgatgagc ggctgcgagc cgatgcgctt gccgacgccg atcttgtaga
     1141 agccggcgaa tccgttggcg atgagcccgg ccgccgcggc ggtgatcgcg atgccgatct
     1201 gctggacctc gtggccgccg ccgagctttc gcaccgcctc gaacgtcgcg tacgcggcgc
     1261 tcgaccagat caccagcacg accagcacgc cggacagatc ttcgaatcgc tgatacccga
     1321 aggtgtagcg ccggtcggcg gcgcgccgcg acgccacgaa cgcgatcgac agcgcgatcg
     1381 tggtgagcac gtcgccgaga tcgtccaggc ccgcggcgag caggccgacg ctgcccgaga
     1441 tgtaggcgat caccaaacgc acgacggcgg aggcgccgag cgcgatcgcg gcgatccaaa
     1501 tcgcccgcag cccggcgcgg gttccttcgg cgccggtcag cgccgcagga tgcgcgtgtc
     1561 cggcgcccac gcgcgtcaat cccgcacgac gatgttgacc agcttcggcg gcaccacgat
     1621 cgtcttgacg accgtcttgc cctccacgaa cgccgcaacc ttgtcgctgg cccgcgccag
     1681 cgcctccatc gcgtccccgg agatgtcggg caccacctcg atcttgtcgc gcaccctgcc
     1741 gttgacctgc acgaccatcg tgagcgagtc gcgagcggcc aaggcgtcga tcgcctcggg
     1801 ccagcgcgcg tggatcaaat ggccttggcc gcccagccgg tgccacacct cttcggtaat
     1861 gaacggcgcg tacgggttca gcatcacgac gagcgcgcgc acggcctcgg cgacgtcggc
     1921 gtcgtcggag gatccggcca gctcggcgat gtcgttggtg agcaccatca gcttcgagat
     1981 cgcggtgttg aaccggaacc gctcggtgtc atgcccggcg ccttggatgg cgcgatgggt
     2041 gcgccgccgc agctcgccct cccccgaggg cgccccggcc gcgacgatcc gtccggcgtt
     2101 ctcgacggcg agccgccaca cgcgcgacag ccagcgaaac acgccgtgcg gcgagacgtc
     2161 ggcccagtcg atgtcggcct cgtacggtcc cgcgaacagc attgtgaccc gggccgtgtc
     2221 ggcgccgaac tcgtccacga tcgccgacgg cgtgaccagg ttcccgcggc tcttggacat
     2281 cttggcgcct tccatgatca cggtgccctg gttgaacagg ttcgggtagg gctcggtgaa
     2341 cgagatcatg ccgaggtcgt agagcgcttt ggtgaagaat cgcgagtaga tcaagtgccc
     2401 ggtcgcgtgc gtgtccccgc cgatgtattg gttggctggc agccaccgct cgacgagctc
     2461 gcgatcgaag ggcgcatcgc cggtgtgcgg gctgcagtag cggaagaagt accacgacga
     2521 gtccacgaac gtgtcgaacg tgtcgatgtc gcgcttgccc gcgctgccgc actgcgggca
     2581 cgaggtgttg acccagtccg tgaccttcgc cagcggcgac tcgccttcct cggtgggcgt
     2641 gtagtcctcc aggtcgggca gcaggaccgg cagctgatcg tcgggcaccg cgacgtcgcc
     2701 gcattgctcg cagtacacga tcggaatcgg acagccccag aaccgctggc gcgacacgtt
     2761 ccagtcgcgc aagcggtagc gcacgcggcc ggtgccgagc tgctgctcct ccagccaggc
     2821 gatcaccttc gcgatcgact ccggcgtcgg cgacccgtcg aagtggcccg agctcgacat
     2881 gcggccctcg ccgtcgtagg cggcctccat ggtctggccg tccagcgcgc cgccctccgg
     2941 ctggatcacc acacggaccg gcaggccttc ctgccgcgcg aactcgaagt cgcgctgatc
     3001 gtgggcgggc accgccatga tcgcgccggt tccgtactcc atcagcacgt agtcggcgac
     3061 gtagacgggc acctgctcgc cgttgaccgg gttgaccgcg tgcgcgccgg tgtcgaagcc
     3121 gagcttgcgg cggcccaggg ccgtgcgctc gatctcgctc gcggccccga cctcgcgcag
     3181 atacgcctcg aaggccccgg ccttgccggc gcgctcggcc aggacgcggg ccatcgggtg
     3241 ctccggcgcg atgacgaaga acgtcacgcc ccagagcgtg tccggccgcg tggtgaagat
     3301 cgtgatctgc tcgccggtct cggcgatcgc gaacgtgacg tcggcgcctt cggagcgtcc
     3361 gatccagttc cgctggatga tcttgacgtg ctcggaccag ccggtgagct ggtccatgtc
     3421 gtcgagcagc cgctgcgcgt aggcggactc gcggaagaac cacgaggtca cgtcgcgctt
     3481 gacgaccgca gcgtcgcaac gctcgcagcg cccttgcacg acctgttcgt tcgcgagcac
     3541 ggtcttgtcg ttcggacacc agttgaccgg cgcgctcgag cggtacgcga cccccgcctc
     3601 gcgcagcttc aggaacagcc actgggtcca cgtgtagaag ccgggatcgc aggtgttgaa
     3661 ccggcgcgac caatcgaagg acatgcccag gcgcttgaac gacttcgcct gctgctcgat
     3721 gttctcgtag gtccacgagc gcggattcgc gccgcgcttg atcgccgcgt tctcggccgg
     3781 caggccgaac gcgtcccagc cgatcggatg gagcacgttg tagccacgca gcatccagta
     3841 gcgcgcgatc acgtcgccgc cgccgagcgc ctccagatgg cccatgtgaa ggtcgcccga
     3901 agggtaggga aacatgtcca gcgcgtagaa gcggggacgc gggtcgttcg ggtcgtcgga
     3961 cgcgcgatac ggctcggtgc gctcccactc atcgacccat ttcggctcga tcgaggacgg
     4021 atcgtagcga tcttcgtcgg tcatgatcgc gccagtttag ccgagcatcc ctgctcgtcc
     4081 cggcgcattc ggcgcccggc cggatagagt ttgcggacca gaggggggcg acggtcccgc
     4141 cgtcgccggt caccgtgggg ggccttgatg aaaccggagc acgtctacga tctgacgggc
     4201 gcgaccgacc cgcgcctttc gcccgacggc cgcaccgtcg cctgcaccgt ctggagggtg
     4261 gaccgcgacg ccaacgccta tcggggcgcg atctggctga ccgcgaccga cggaagcgag
     4321 cccccgcgcc gcctcacctc gggggagcac cgggacggct cgccgcggtg gtccccggac
     4381 ggcacgatgc tggcgttcgt ccgcacgcgc ggcgacgtca aggacgccga ggcgcagctc
     4441 tgcgtcctgc cgctcgccgg cggcgagcca cgcacgctga cggatctgaa ggagagcgtc
     4501 gaggatccgt cctggtcgcc ggacggctcg acgctgctgt tcgtcgcgcg aacccacgat
     4561 cccgacgacg acgaacgcga tgagcgccgc cgcgcgccgc gccgcatcac gcgccttcag
     4621 ttcaagctgg actcggtcgg ctggaccgcc ggccgccgcc agcacctcta caccgttccc
     4681 gccgacggct cggctccggc cacccggatc accgacggcg acttcgagga cgccgatccg
     4741 tgctggtccc ccgacggaac acagatcgcg ttctgctcgg cccgcgacga ggactgggac
     4801 accaccatgg cgcgggacct gttcgtgatg ccggcgaacg gcggcgagcc ccgcaagctg
     4861 accgcatccg acggggcggc atcgtcgccg tcgtggtcgc cggacggccg ccgcatcgcg
     4921 tgcctgtggt acccggtcgt gttcgacgac ccgcgtcaca cccaggtcgc ggtcttcgac
     4981 gccgcctccg gcgagcgcac cgtgctcacc gactcgctcg accgcaactg cggcatctac
     5041 cccacgctcc gcgagccgat ctgggacggc gaggacctga tcttcgcgac cgaggactcg
     5101 ggcaacaatc ccgtctatcg cgttcgcgcg gacggctccg gcaagcccga gccgatcctc
     5161 gaaggcgact tcatgctcac cggcctcgac gcggccgccg gcgtcgtggt gcacgccgcg
     5221 acgaccgcgg tcacgccgtc ggagatcttc aacggcgcga cccgcctgac ctcggtcggc
     5281 gatgcgctgg tcgccgcgat ccggccgcag acgcccgagc ggttcaccgc gatctccgcg
     5341 gacggctcag aggtcgaggc gtggatcatc ccgcccgcga acgccgagcc cggcgcgcgc
     5401 tatcccacgc ttctgaacat ccatggcggg ccgttctcgc agtacggcaa caaactcttc
     5461 gacgagttcc aggtccaggc cggcgcgggc tacgccgtcg tctacgcgaa cccgcgcggc
     5521 tcgtccggct acagcgaggc gtggggacgc gcgatccggc ccgccgacgg cagcggctgg
     5581 ggcggggtgg acttccagga cctgatggcg gtcatggacg aggccgagcg gcgcttcaac
     5641 ttcgtcgatc ccagccggac cggcgtgctc ggcggatcct acggcggcta catgacgtcc
     5701 tggatcgtcg cgcacaccga ccgcttcaag gcggcctgct cggagcgagc cgtcaacaac
     5761 ttcgtgatgg agggcggctc ggccgacctg ggatggatgt tccagggcga gttcggcacg
     5821 acctggtacg aagatccgca cgcatacttg aagatgtcgc cctcgatgta cgcgtcgagc
     5881 atcacgacac cgctgctgat ccttcactcc gaaagcgacc tgcgctgccc cgtcggccac
     5941 gccgaggacc tgttcacgat ccttcgcctg cgcggccggc cggtcgagat ggtccgcttc
     6001 ccggccgaga gccacgagct gtcgcgtgcc ggctcgccgg tgcaccgcgt ccagcgcttc
     6061 gagatcatcc tggaatggtt cgaccggcat ctgaaagatc cggcgtgacc gacgaggtcc
     6121 tcgtcgagtc cgacgggccg gtgcgagtgg tcacgatcaa ccggcccgcc aagcgcaacg
     6181 cgctccacat ccagctgcgt cgcgcgctcg cgacggcctt tgcgcggctg ggaaccgatc
     6241 cggacgtcgg ggtggtggtg ctggccgggg cgggcagcgc gttctgctcg ggtatggaca
     6301 ccactcagtt cggcggcgac gacgacaacc gccgcgcgct gttcgagtcg tcctacggcc
     6361 tgttcgaggc gctgcggacg cttccgaagc cggtcatcgc ggcggtgaac ggccccgcgc
     6421 tcgccgccgg cttcgcgctc gccgcgttcg ccgacatccg catcgccggt cccgcggcga
     6481 cgttcggcat gcccgaggtt tcgctcggct tcgcctcctc gtgggccacg ctcgcgcgcg
     6541 tgctgcccga tcagctcgca cgccggatgg cgttcacggg cgagacgatc gacgcggcca
     6601 ccgcgctgga atggggcctg gtgatggagg tcgcaccgga tccgctcgcc cgggcccgcg
     6661 cgctcgccgg cgtgatggcc gccaatcccg tgccgggact ggagggcacg aaacggctga
     6721 tggtcgagtc cgccgacccc gcgctgcggg ccgcgtggga gaccgagatg cgaatcttcc
     6781 acacctcact gttccgcaag cggtgaaaca cctcgccttt ccgggggtcc tgatgagaaa
     6841 cgcctcgcca ggggccgacg gcaggaggga acgacatgaa aaagcttctc gcagcagtgg
     6901 ccgtcgccgc gatgctcgcg ccgggctcgg cgggcgcggc caccgacgtc gccgcggttg
     6961 ggcaagggaa cggcacgatc acgcgcacca acgggacctg cagcgcgccg ctcgcgctga
     7021 ccttcaacgt gcgcagcaac gtcgcggccg tgaccgagag ctggggtccg gggggtccga
     7081 acaccgagtc gtgcgcgctc gcgacgatac tccgcctgta cccgaccacg accgcgccgg
     7141 tgccggcggt tcagcagtgc ctcccgggaa tcagcatcca tagcgggggc gcctggacgc
     7201 tcagccaatc cggccagacc tatacgatgc agcgcacgta ccggctctgc aacggcaagc
     7261 agatcttcga caagatcgtc gtcaccgtgc aggccacacg tctcgtctac agcaatacgt
     7321 acgccgagaa cggagtcacg ctggtgacgg tgaccgcgaa cgtgccacgc atcgcatgag
     7381 gggagatcga tgaagcgtct tgcactcgtc ctgacggctc tggcattgct ggggccgtcc
     7441 tcatcaccgg ccgcgagtga cgcgacggcc gccggtcacg gaaacggctc ggccacctac
     7501 gcgggcgtca cctgcaacgc gccggtcgcg atcacgttcg tcgttcgcgc cagcgccgcc
     7561 gtcatctccg atacgtgggg tgccggcaca acccccgccg cgtgcactcc ggtcggaggg
     7621 gtgatggtgg cgggctcgtc ggcgctgagc cagtccggct cgacgtacac ggcgcagtcc
     7681 tcctacaccg gcaccgacgg cgcgccgatg accgacaagg tcgtggtcac cgttcagtcg
     7741 aaccggttgg tttattcgcg cacggtgacc aggagcggga tcgtcgtcga aagcgcgtcc
     7801 gccaacgtcc cgcgcgtggc atagccgcgc gcgggacgtt gttcggaatt ttccacgccc
     7861 cctcgtgcat cgacgctgaa acgctcccgc ctcgaaagtg tcctttgctg tgagacgcgg
     7921 cccactgggg gccggctggg ggaaacgaca tgaaaagact gcttcttgcg cttgcggccg
     7981 ttgcgcttct ggtgcccgca gcggctccgg cggccaccga cgttgccgcc gttgggcaag
     8041 ggaacggcac gatcaaggtc aacaacctga cgtgcgcggc cccgctggcg gtcacgttga
     8101 acgttcgcag caacgtcggc gtcgtgaccg agaactgggg cccgggcaac ccgagcccgc
     8161 tcctcgactg catcttcatg ccggtcttcg gccaatatga ggccgacaac accccggttc
     8221 cgccggttca gcagtgcgtg cagggagccg ggctttcgac gggcatgccc tacgtgctct
     8281 cgcagtcggg atcgacctac acgttcaccg gatcgacacg tctgtgcaac gggaagcagg
     8341 tgctcgaccg ggtctccatc accgtgcaga cgacccggct cgtctacagc cacacctggt
     8401 ccgagaacgg cgtggcgatc gtcacggtca acgcgaacat ccctcgtctc gcctgaccag
     8461 tccccggacg aatccgggcg aaccctccgg agcggcgcgg cccgaccctc tctacccctg
     8521 aacggcggga cgcgccgcct cccctatctc cgggcacggc aaagacaggc catcctcggg
     8581 atttaccgcg actttccaaa cgagttgcag gcgttctacc aggggagaca cggctaagag
     8641 gccttccggt cgcttgcggt cccttcgctc tggcgatcgg aatcggcgaa gcggacaatg
     8701 ggataacctc gtgccgagga aaaccaatgc agatcgccga tgtccagatc cgcgaggcgg
     8761 cgcgcgagga cgagagcgcg cttaccgagc tcgcctacga gtatctgagt tgggcgatcg
     8821 agcgactgcg atcggatcgc ggcgttgagt ggcccgcgat tacgagggat gacgtggccg
     8881 aggccatcga ctcctaccgc aacgggggcg cgatcctgat cgcgacggtc gatgccgcac
     8941 cagtcgggat gggcgcgttg cgacggctgg atgaaggcgc gttcgagatc aagcgcatgt
     9001 acgtgcgacc ggccgcgcgc ggccatcgcg tgggcgcgcg catcctggat gacctgctcc
     9061 tgcgcgctcg tgcagcggag gggcgcgtgg tgaggctcga caccatcgtc ttcatggacg
     9121 acgcgcagag gctctacagg tcgcgaggct tcgtgcagcg aggaccttac gaagaaagcg
     9181 agatcccgac acggctccag gcgcactggc tgttcttcga gcttgctctc cgctgagctg
     9241 cgtgttcgtc gtacgagggc acgcgaggac ggcgcacccc ctatggtcag ccctggccac
     9301 aagtttgaaa gcctgggacc cgtcggcagg atccggttcg aacgtcgtca ctcagttgcg
     9361 agcgtcatac ccggaaaacg tgtctgtgga gctgagggga ctcgaacccc tgaccccttg
     9421 gctgccagcc aagtgctcta ccaactgagc tacagcccca ggaacgctgc atgttatcac
     9481 ccgggcccac accccacatc ctcgggcgcc ccgggatctg cggccgcgcg tttcgcgatt
     9541 gcgcgcagcg ggaaaacaac cttcgctatg tggctatgga ttccgggagt accgctcggg
     9601 ctggggctca tgctctgggc cttgagccgc ttggagacgt tcttcgtcga gccggtcgag
     9661 catgccgagc gcatctcgaa gctaatgcgt caggccgaca cggaagggct cgaggccgag
     9721 gtcgcccggc tgctggcgcc ctccgtcgag gtcgcgaccc ggcgttcggc tcgccggcga
     9781 agcctcgccg ctacctagat cgttcctggt cggcggcatg cccgtggcac ggcggcgata
     9841 gccggtcctc gcggggccta cttctttcgt cggatcgggc gcagcacgtc gagacctcgc
     9901 gtcatgtctc ccatgaacac gtagcccttg tgctcgtagg ctccccacgc ggtcgcgcct
     9961 tcggcgacgt acgaaccgat cactttcggt gctcgcggat cggagtaatc gatgacctgc
    10021 agacctgcgt tgtaggcgcc catgtagacg gtcttgccct tcgacgagaa cacgtgcgtc
    10081 gagcagatgc cttcggcggc gccggcggga accttccatt cgctgagctc gtgggcgtcg
    10141 gtcatgttcg cccacagccg cacggccgtg atcccgcccg agacgccgca gccgctgtgc
    10201 atatcctcgt cggtgacgag catcacatgc ttgtcaccgt caagcagctg cgcctcgtgg
    10261 ttgtagcgaa tggggtggtg gccgactccg gtttcgtgga tccaccgcag catcacgacc
    10321 ggccgtgacg gatcggtgat atcgatcacg aagatgctcc cggtgttgcc ggcgtcgttg
    10381 aggtagtccc cgccttcggc gagcagcgcg atgtccttgc cgcggaacgt cgggtcgggc
    10441 ttgccgtccc ggttgaggtc gatccattcg cggcgaggca cgagcaccgg gtagtcggtg
    10501 tagacggtga tgtcatgcgt gtgcgtgaac gcgggcccga cgtcgcggcc gcgccggtac
    10561 ggcgacgcgc gccacatcgt gacgggattg ccggcgaaaa cctcgtccgg ggccttcggc
    10621 tccggcagca gcagcggccg catgtcgtag atgcggagcc cgccgcgccc tttcgccccg
    10681 gagggcagcc acagcttgcg cttgtcgacg atgtcgccgt tgtgcgactc gccgtgatcc
    10741 ggcccttcga aggtccacag cagttttggg ttggccgcgt cggtgacgtc gaggaactcg
    10801 gcgcgatcgg tgcgctccgc acgcttggtg ccgttgagca ccgcgatgtg ccggccttcc
    10861 cagaccgccg cgtcgggaac ggcgtcgaat cgcgcgcccg gcatgtactg accgatcaag
    10921 gccggcgcgg acggatcctt gatgtcgtag atgcgcatgc cgagcccgta ggagcccaga
    10981 tacagccgat cgccttcgat ggcgatgtgg ccgcccggca gcccgttgtc gcccgtgacg
    11041 tactcgacgt cgagcagatc ggctgcgagc ggcggcaacg gctccgcccc ggacgtcatc
    11101 gccgacgcga gaatcgcgac gacggcggcc agcagggaaa cacgacgtct cacacacacc
    11161 tccgaggatc ggtcccggac ttgttc
//
LOCUS       DATLFO010000353         3155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_118030, whole genome shotgun sequence.
ACCESSION   DATLFO010000353 DATLFO010000000
VERSION     DATLFO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118030"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="map"
                     /locus_tag="VM841_14345"
     CDS             <1..789
                     /gene="map"
                     /locus_tag="VM841_14345"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVL91406.1"
                     /translation="HRRRDRMIITKNDREIATMRRAGEVVAEMKAAVRAALRPGVSTA
                     DLDKAAAQVVEARGATASFLGYRGFPATICASINDEIVHGIPSPDRILQDGDLFKGDF
                     GAIVDGFHADSAFTLPVGEVSDELMKLIETTERALWAGIAEVRPGNRMGDIGHAIQTV
                     AEGAGFSVVREYVGHGIGRALHEDPPVPNYGTPGKGVALKAGMVIAIEPMVNVGTYET
                     RLMPDKWTVKTADGAMSAHFEHTIAITEAGPWVLTDPDQPEPPR"
     gene            889..1002
                     /gene="rpmJ"
                     /locus_tag="VM841_14350"
     CDS             889..1002
                     /gene="rpmJ"
                     /locus_tag="VM841_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVL91407.1"
                     /translation="MKVRPSVRKMCEKCRVIRRHGRIMIICSNPRHKQRQG"
     gene            1018..1401
                     /gene="rpsM"
                     /locus_tag="VM841_14355"
     CDS             1018..1401
                     /gene="rpsM"
                     /locus_tag="VM841_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVL91408.1"
                     /translation="MARIAGVDLPRDKRVEIALTYIFGVGPTLSRKTLAAAQIDPGTR
                     VRDLSDDEVLRLREWIDANYKVEGDLRRQVNQDIKRKIEIGCYQGIRHRRGLPVRGQR
                     THTNARTRKGPKKTVAGSKKVRAKK"
     gene            1417..1803
                     /gene="rpsK"
                     /locus_tag="VM841_14360"
     CDS             1417..1803
                     /gene="rpsK"
                     /locus_tag="VM841_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HVL91409.1"
                     /translation="MAKPKKTKRKEKKNVPYGHAHIQSTFNNTIVTITDPQGNTLSWA
                     SSGNVGFKGSRKSTPFAAQLAAEAAAKRAMDHGVRKVDVYVKGPGSGRETAIRSLAAS
                     GLEVAAINDVTPVPHNGCRPPRRRRV"
     gene            1804..2430
                     /gene="rpsD"
                     /locus_tag="VM841_14365"
     CDS             1804..2430
                     /gene="rpsD"
                     /locus_tag="VM841_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HVL91410.1"
                     /translation="MARYNDADCKLCRREKIKLYLKGTKCETPKCPVEKRPFPPGQHG
                     RARTKETEYLIRFREKQKAKRIYGLLEKQFRNTYDLASRMKGITGENLLSLLELRLDN
                     VLMRAGFATSRDQARQLVSHGHVRVNGRKLSIPAARLRVGDTIEIAEKSRRMAPVLHA
                     LAVTENRSAPGWLRVERDAWRATVSDVPARADIDVPVQEQLIVELYSK"
     gene            2456..>3155
                     /locus_tag="VM841_14370"
     CDS             2456..>3155
                     /locus_tag="VM841_14370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HVL91411.1"
                     /translation="MLIVQRPQIVEEPVSDTRWRFSVEPLEPGFGYTLGNALRRTLLS
                     SIPGAAVTQVRIEGVLHEFSTINGVKEDVTDVILNVKDLVIRSTLDEPVALTLRVQGP
                     KDVTAEDIAPVAGVEILNPELHIATVNKKGTFEMELVVERGRGYVSADRNAKGRDTIG
                     LVPIDSIFSPVRRVAYAVEHTRVEQMTNYDKLVLDIETDGSISPKEAFAAAGRTMVEL
                     LQLVAMLAEDAQALQ"
BASE COUNT          580 a         1066 c         1036 g          473 t
ORIGIN      
        1 catcgccgcc gagatcgcat gatcatcacc aagaacgacc gcgagatcgc gaccatgcgc
       61 cgtgccggcg aagtcgtcgc cgagatgaaa gccgccgtgc gcgccgccct gcgtcccggt
      121 gtatcgaccg ccgacctgga caaggccgcg gcccaggtcg tcgaagcgcg cggcgcgacc
      181 gcctcgttcc tggggtaccg cggcttcccc gccacgatct gcgcgtcgat caacgacgag
      241 atcgtgcatg gcatcccctc gcccgaccgc atccttcagg acggcgacct gttcaagggg
      301 gacttcggtg cgatcgtgga cggcttccac gccgactcgg ccttcaccct gcccgtgggc
      361 gaggtcagcg acgagctgat gaagctgatc gagaccaccg agcgggccct gtgggccggc
      421 atcgccgagg tccggcccgg caaccggatg ggcgacatcg gccatgccat ccagaccgtc
      481 gccgaaggcg cgggtttttc ggttgtgcgc gagtacgtcg gccacggcat cggccgggcg
      541 ctgcacgagg atccgccggt cccgaactac ggcacacccg gtaagggggt cgccctgaag
      601 gccggcatgg tcatcgcgat cgagccgatg gtcaacgtcg gcacctacga aacccgtctc
      661 atgccggaca aatggaccgt caaaaccgcc gacggagcca tgtccgccca cttcgagcac
      721 accatcgcca tcaccgaggc aggcccttgg gtcctcaccg accccgatca gccggagccg
      781 ccgcgatgat gggccgcaaa ggcgggttgt cgcaggtcag cgggcctgct agcatgcccg
      841 tttggtcgcc cggccgggtc ggccgacacc caaggagcgc ctagggctat gaaagtacgt
      901 ccgagtgtcc gcaagatgtg cgagaagtgc cgcgtgattc ggcggcacgg ccgcatcatg
      961 atcatctgct cgaacccgcg gcacaagcag cggcaagggt aaggggagtt tccgcttatg
     1021 gcacgtatcg ccggcgttga tctgccccgc gacaagcggg tcgagatcgc gctcacctat
     1081 atcttcggtg tcggcccgac gctgtcgcgc aagacgctcg ccgccgcgca gatcgatccg
     1141 ggcacccgtg tccgcgacct gtccgacgac gaggtccttc gccttcgaga gtggatcgac
     1201 gcgaactaca aggtcgaagg tgatcttcgc cgtcaggtga accaggacat caagcgcaag
     1261 atcgagatcg gctgctatca gggaatccgc caccgccgtg gcctcccggt gcgcgggcag
     1321 cgcacgcaca ccaatgcgcg cacgcgcaag ggtccgaaga agacggtcgc gggttccaag
     1381 aaggtccgcg cgaagaagta atcaggagat ctttcagtgg cgaagcccaa gaagacgaag
     1441 cgcaaggaaa agaagaacgt gccgtacggg catgcgcata tccagtcgac gttcaacaac
     1501 acgatcgtca ccatcaccga cccccagggc aacaccctgt cgtgggcctc gtcgggcaac
     1561 gtcggcttca agggctcgcg caagtccacg ccgttcgcgg cgcagctcgc ggccgaggcc
     1621 gcggccaagc gcgcgatgga tcacggcgtg cgcaaagtgg acgtgtacgt gaagggtccg
     1681 ggctcgggcc gcgagacggc gattcgttcg ctggccgcgt cgggcctgga ggtcgcggca
     1741 atcaacgacg tgacgccggt tccccacaac ggctgccgcc cgccgcggcg gaggagggtg
     1801 taagtggcgc ggtacaacga cgcggactgc aagctctgcc gccgcgaaaa gatcaagctg
     1861 tatctgaagg gcaccaagtg cgagacgccc aagtgccccg tcgagaagcg tcccttcccg
     1921 ccgggccagc acggccgtgc gcgcacgaag gagaccgagt acctcatccg cttccgcgag
     1981 aagcagaagg ccaagcgcat ctacggcctg ctcgagaagc agttccgcaa cacctatgac
     2041 ttggcgtcgc ggatgaaggg catcaccggc gagaacctgc tcagcctgct cgagctccgg
     2101 ctcgacaacg tgctgatgcg cgccggcttc gcgacctcgc gcgatcaggc tcgccagctg
     2161 gtgagccacg gccacgtgcg ggtcaacggc cgcaagcttt cgatcccggc ggcgcgcctt
     2221 cgcgtcggcg acacgatcga gatcgccgag aagtcccggc gcatggcgcc ggtgctgcac
     2281 gcgctggccg tgaccgagaa ccgcagcgcg ccgggctggc ttcgcgtcga gcgtgacgcg
     2341 tggcgtgcga ccgttagcga cgttcctgct cgcgccgaca tcgacgtgcc ggtgcaagag
     2401 cagctgatcg tcgagctcta ctcgaagtaa cgatccccgt gggagggatg ctttcttgct
     2461 gatcgtccag agaccgcaga tcgtcgagga gcccgttagc gacactcgct ggcgcttctc
     2521 cgtcgagccg ctcgagcccg gcttcggcta cacgctgggg aacgcgctgc gccgtaccct
     2581 tctttcctcg attcccggcg ctgccgtcac gcaggtccgt atcgaaggcg tcctgcacga
     2641 gttctccacc atcaacggcg tgaaggaaga cgtcaccgac gtgatcctga acgtcaagga
     2701 tctggtgatc cggtcgacgc tggacgagcc ggtcgcgctg acgctgcgcg tccagggccc
     2761 gaaggacgtc accgccgagg atatcgcgcc ggtcgccggc gtcgagatcc tcaaccccga
     2821 gcttcacatc gcgacggtga acaagaaggg caccttcgag atggagctgg tcgtcgagcg
     2881 tggccgcggc tacgtgtcgg ccgaccgcaa cgccaagggc cgcgacacca tcggcctggt
     2941 cccgatcgac tcgatctttt cgccggtgcg ccgcgtcgcc tacgcggtcg agcacacgcg
     3001 cgtcgagcag atgaccaact acgacaagct ggtcctggat atcgagaccg acggctcgat
     3061 cagcccgaag gaagctttcg ccgccgccgg tcgcaccatg gtcgagctgc tgcagctggt
     3121 cgcgatgctc gccgaggacg cgcaggccct gcagc
//
LOCUS       DATLFO010000354         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_98706, whole genome shotgun sequence.
ACCESSION   DATLFO010000354 DATLFO010000000
VERSION     DATLFO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98706"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VM841_14375"
     CDS             <1..228
                     /locus_tag="VM841_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVL91412.1"
                     /translation="GIGIASLINVLDPSVVIVGGGAAAGTGDLLLEPARAAMREHLTL
                     REHRPPVEILPAALGNDAGAVGAADLARSVL"
     gene            271..>2160
                     /locus_tag="VM841_14380"
     CDS             271..>2160
                     /locus_tag="VM841_14380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVL91413.1"
                     /translation="MEAKTEAEARVVELDLEGMTCASCATRIEKVLRRQEGVTEANVN
                     FAAERARVAGEAELDRLIDAVAKAGYRARPSKPSRGSSETAAAEAVFRRFLVAAILTA
                     PAMAIGMIWSEARWAMAATWVLVTPVQFWSGWPFLRSAALNARHGVATMDTLVAVGTL
                     AAYTYSAWVTAMGHGHAYFETAGTIITLILLGKYFELTSRGRASSAIRALLERGAKQA
                     RVLREDGSEVMVDVAELAPGDVFIVKPGEKIATDGIVRSGSSAVDESLVTGESVPREK
                     SAGDEVIGGTVNAQGVLTVEATKVGADTALAQIARLVEEAQGSKAPIQRLADRVAGVF
                     VPIVLVIAAATFAFWLLSGDPVGEALIPAVAVLIIACPCAMGLATPVAIMVGTGRGAQ
                     MGVLIRGGDVLERSRSIDAVVLDKTGTITEGRMRVTDVEVDPWNGGSVGSGEVLAAAA
                     AVEQASEHPIAAAVVRAAKERGLAIAPVEGFEAAGGNGARGMAGGREIFVGRASYLGE
                     NGLMSCSELDEHKAKLEAEGKTVFGVGWERRVRGLIAVADTIKPTSKAAVAALRRAGA
                     DVVMITGDNAATAKAIAASAGRSRHRDRQRHRRRDRGERYHARGRRPAWRADRDRARA
                     AHLSQH"
BASE COUNT          324 a          737 c          803 g          296 t
ORIGIN      
        1 ggcatcggca tcgcgagcct gatcaatgtg ctggatccct cggtggtcat cgtcggcggg
       61 ggagcggccg ccgggaccgg cgatctgctg cttgagccgg cgcgcgcggc gatgcgcgag
      121 catctgacgc tgcgcgagca ccggccgccg gtggagatcc tgcccgccgc gctcggcaac
      181 gacgccggcg cggtcggcgc ggccgatctg gcccgttccg tcctgtgatc cggcttgcaa
      241 ggtaccccag gggggtatag agtcgctcgt atggaagcca agaccgaagc ggaagcaagg
      301 gtcgtcgagc tcgacctcga aggcatgacc tgcgcgtcgt gcgccacgcg catcgagaag
      361 gtgctgcgcc gccaagaggg cgtcaccgag gccaacgtga atttcgccgc cgagcgagcg
      421 cgcgtcgcgg gcgaggccga actcgaccgg ctcatcgacg cggtcgccaa agccggctac
      481 cgcgcccggc cgagcaagcc gtcccgcggc tcgagcgaga ccgcggccgc tgaagcggtc
      541 ttccgccggt tcttggttgc cgcgatcttg accgctccgg cgatggccat cggcatgatc
      601 tggagcgagg cacgatgggc catggccgcg acctgggtgc tggtcacccc ggtccagttc
      661 tggtcgggat ggccgttcct tcgcagcgcc gcgctcaacg cgcgtcacgg cgtggccacg
      721 atggacacgc tcgtcgcggt gggaacgctc gcagcctaca cctacagcgc ctgggtgacc
      781 gcgatgggcc acggacacgc ctacttcgaa accgcgggca ccatcatcac cctgatcctg
      841 ctcggcaagt acttcgagct gacctcccgc ggacgcgcgt cctcggcgat ccgcgcgctc
      901 ctcgagcgcg gcgccaagca ggcgcgggtt cttcgcgagg acggcagcga ggtcatggtc
      961 gacgtcgcag agctcgcgcc cggcgacgtg ttcatcgtca agccgggcga gaagatcgcc
     1021 accgacggca tcgtgcgatc gggatcgagc gccgtggacg agtcgctcgt gaccggcgag
     1081 agcgtgccgc gtgaaaagag cgcgggcgac gaggtcatcg gcggcaccgt caacgcacaa
     1141 ggcgtgctca ccgtcgaggc gaccaaggtc ggcgccgaca ccgcgctcgc gcagatcgcg
     1201 cggctggtcg aggaggccca aggctcgaag gcgccgatcc agcggctcgc tgaccgtgtc
     1261 gcgggcgtgt tcgttccgat cgtgctcgtg atcgccgcgg ccacgttcgc gttctggctg
     1321 ctgagcggcg accccgtcgg cgaggcgctg atccccgcgg tcgccgtcct gatcatcgcc
     1381 tgcccgtgcg cgatgggctt ggccacgccc gtggcgatca tggtcggaac cggccgcggc
     1441 gcgcagatgg gcgtgctgat ccgcggcggc gacgtgctcg agcgctcgcg gtccatcgac
     1501 gcggtcgtgc tcgacaagac cggcacgatc accgagggcc gcatgcgcgt caccgacgtc
     1561 gaagtcgacc cgtggaacgg cggctcggtg ggaagcggtg aggtgctcgc cgcggcggcc
     1621 gccgtcgagc aggcgtcgga gcatccgatc gccgcggcgg tggtgcgcgc ggcaaaggaa
     1681 cgcggcctcg ccatcgcgcc ggtcgagggc ttcgaggccg ccggcggcaa cggcgcgcgc
     1741 ggcatggccg ggggccgcga gatcttcgtc ggccgggcga gctatctggg cgagaacggc
     1801 ctgatgagct gcagcgagct cgacgagcac aaggcgaagc tggaggcgga gggcaagacc
     1861 gtcttcggcg taggatggga gcgccgcgtg cgcggcctga tcgccgtcgc cgacacgatc
     1921 aagccgacct cgaaggccgc cgtcgccgcg ctgcggcgcg ccggcgccga cgtggtgatg
     1981 atcaccggcg acaacgcggc gaccgcgaag gcgatcgcag cgagcgcggg ccgatctcgg
     2041 catcgcgatc ggcagcggca ccgacgtcgc gatcgaggcg agcgatatca cgctcgtggg
     2101 cgacgacctg cttggcgtgc cgaccgcgat cgtgctcgcg cggcgcacct atcgcaacat
//
LOCUS       DATLFO010000355         9277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_118157, whole genome shotgun sequence.
ACCESSION   DATLFO010000355 DATLFO010000000
VERSION     DATLFO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118157"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VM841_14385"
     CDS             complement(<1..491)
                     /locus_tag="VM841_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL91414.1"
                     /translation="MNRRSAAALTLVAALIAVVVPLDRAAAGQSIVAARRAVFGAANV
                     ADDGRVRGDRVILSWFGVTNFAAAIGGHVVLLDAWVPRGEYSGYVPASPETLAAIKPE
                     YIFAGHSHFDHLADAAEIAVASGAKLVGTPEHCDQVRRQAGMAPVVCVEAVARGAARD
                     RLHA"
     gene            595..2238
                     /locus_tag="VM841_14390"
     CDS             595..2238
                     /locus_tag="VM841_14390"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HVL91415.1"
                     /translation="MTDTHEVINQAEPLLGHNWYRSDRALVEAVHREGGAWAEDRIDA
                     LGAKAASPEATTWATQANVYPPVLRTHDRVGNRIDEVEFHPAWHELMRVSVGAGTHAL
                     PWREPRAGAHVARAAMMMVWQTDAGHGCPISMTYSVIPALRKQPELAAEWEPRLLSLD
                     YDERFVPAPDKRGALAGMAMTEKQGGSDVRANTTRAVPVNGGGPGAEYEITGHKWFCS
                     APMCDMFLVLAQAPGGLSCFLLPRWRPDGTRNAFRIQRLKDKLGNRSNASSEVEFHGA
                     YARMVGEEGRGVPTIIEMVNHTRLDCVLGSTAGMRYGLMNAIHHTSQRAAFGRVLIEQ
                     PLMQNVLADLAVEAEAATVTAMRLARAYDAAADDEQEALFKRVATAIAKYWICKRQPA
                     HVGEALECFGGNGYVEDSGMPRLYREAPLNSIWEGSGNVICLDVLRAFAKTPGSLDAF
                     FAEVSLAEGADARLDAYVKDLRNELATPGDMESRARRLVERLALALQGSLLVRHSPPE
                     IADAFSASRLAGDWGRAFGTLPSRLDLPVIVERATPIWD"
     gene            2775..2927
                     /locus_tag="VM841_14395"
     CDS             2775..2927
                     /locus_tag="VM841_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein"
                     /protein_id="HVL91416.1"
                     /translation="MAAILVLAIILALFGGGFALEILWYVAIAALILWVVGFLARGSG
                     ARWYRW"
     gene            3043..4602
                     /locus_tag="VM841_14400"
     CDS             3043..4602
                     /locus_tag="VM841_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVL91417.1"
                     /translation="MAGRDTLRLADLLAAVSVATDLGMGQTPEKAIRSCLVATQFAGT
                     LGMPGPVVHDVYLTTLLRHLGCTATAHEEAYLFGGDERASRPPAERTDFGSVREAAAL
                     MMATGRGAGLGRPRYLARAIRAGKQGTDAILRAICEIASRLAARLELGPGVEEALYHV
                     HERWDGTGSPAKLRGDDIALPARVAEIATQAIIFDAAAGPDAAVAMVRRRAGGWFDPA
                     LAAEFERVGPAILAEVRASDPWVAVLDAEPAPNRTIRSADLERLARAFADMVDLKSPY
                     TLGHSSETAALAVAAARTLAMDDDDCRDILHATLLHDLGRTGVSNGVWDKPGPLSTSQ
                     WEQVRLHAYHTERIMMRSAALAPLAPIAGAHHERQDGSGYHRASNASAIPPAACLIAA
                     ADAFQAMTQDRPHRPALSPEVAAKALAADATAGRLDPTCVRAVIDAAGQSPTRVRIPW
                     PAGLTDREVEVLRLVALGSSNRELAARLFISPRTAEHHVQSIYAKIGASTRASAALFA
                     MEHGLYRAGDK"
     gene            4667..4966
                     /locus_tag="VM841_14405"
     CDS             4667..4966
                     /locus_tag="VM841_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91418.1"
                     /translation="MYARINTFEGAPEGIDASVEHVRTMVLPVARGIDGFAGLLALGD
                     RTTGKTIGITFWRSLEAMHASEQAADRLRDEVAAASNERVVSIEQYEVLLDEKGP"
     gene            4968..5426
                     /locus_tag="VM841_14410"
     CDS             4968..5426
                     /locus_tag="VM841_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVL91419.1"
                     /translation="MAAIDFVIRTTTSPDTVFAALTDFTSRRPALWPALDPDVYHVEM
                     LGETRALVREGQRSPRLWALEEYDWSTPGTVTWTARESNFCAPGSLMSATISPESGGG
                     SAVAFRWSRRGIGVKGHLIVAMVRATKGRPLVAGLSKALDGLAAKEQQAR"
     gene            complement(5423..6205)
                     /locus_tag="VM841_14415"
     CDS             complement(5423..6205)
                     /locus_tag="VM841_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HVL91420.1"
                     /translation="MSEEIVDHDNAPADATREEKAALLFPRRGEPGVREELVRLFYPL
                     AEHLARRFAGRGESYDDLVQVASIGLLKAVDRFDVERGVRFDTYAVPTIVGELKRHFR
                     DTGWAMRVPRRLQDQTLRLRTVIAELGQELGRSPRIPEIAVRSGLSEDDVIETLDAVH
                     ALSVGSIDAPVAEDEQGVAETLGSDDAAMAMTEQWADVAPLLRRLPERERHLLYYRFV
                     LDLSQSEIADKLGISQMHVSRLLSRTFAMLREAAGEETIPTR"
     gene            complement(6205..6678)
                     /locus_tag="VM841_14420"
     CDS             complement(6205..6678)
                     /locus_tag="VM841_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91421.1"
                     /translation="MVPVVVACQTIGNSHRVACFDEEATGYQQADDRGLSMGSVTLTL
                     PARPEYVHIARSVAAGLAAKAEMTLDDIEDIRLAVDEACTHLLAHSHAELLRLELARS
                     GGAFEIVAQIDAVIDDGNGSGDASISWRILEALADSVLLERDAGSAAIRMSKRIS"
     gene            6709..7053
                     /locus_tag="VM841_14425"
     CDS             6709..7053
                     /locus_tag="VM841_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL91422.1"
                     /translation="MDLVVDSRTEGTAAVLTVGGEIDLFTAPKLRERLSGTIDEGMDR
                     VVVDLEGVGFMDSTGLGTLVGGLKRIKERDGSMSLVCTNRSVLRILSITGLDRVFPVY
                     SSVTEALSADAR"
     gene            7092..7484
                     /locus_tag="VM841_14430"
     CDS             7092..7484
                     /locus_tag="VM841_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91423.1"
                     /translation="MNQEDQHDRLVIELPAEAEAVGSARLFATSVARLFGVDDEVVED
                     IRLAISEAVTERVQSAASEDAPVCVSTIVREKELVFEISGGSRTAGENGELGIAVVHS
                     LFPDAVVEQDPHVLRFVAPRATAGSQAP"
     gene            complement(7481..>9277)
                     /locus_tag="VM841_14435"
     CDS             complement(7481..>9277)
                     /locus_tag="VM841_14435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVL91424.1"
                     /translation="AILKADGSVTTVAASVRPADTPSGRFFVSYLRDVAPTRAAKDEM
                     LRLIRDRELLLNAAGEGIIRVDVDGRVTFVNPAAEELLGWERGGLIDRPVHETIHHSA
                     PDGSPYPAEECPINEAARDGSVHRIESEVFWRKDATSVAVEYISAPLWEDGRLAGSVV
                     TFRDVTERRAIEAEVRRARDVFRLLARISSVLGETPLDPDQRLRVLAREFVPMLVDWC
                     AIDLLDNGGLRRVVVEHVDPRKVALAHELQRRYPPDPASEVGAWAVMRTGRPQIFEHV
                     PDEMLEAVARDDEHLDILRSLGLDSVVVVPMTARDRVLGVITLVTAESGRRLNEDDAI
                     LYSEVAARAGILIDNARLFTERSRVAQVLQQSLLPPVLPVIEGVDVAARYVSATNGID
                     VGGDFYDIFQTDPGDWAVVLGDVSGKGAEAAAMTALARYTIRAAAISARKPSAVLTTL
                     NRAIVQQDTGRFCTACYIRVRPSPTGVRLAIARGGHPRPLVLRAGGRLEEAAGEGMVL
                     GLFEDPDLGDRTARLKPGDSLILFTDGVTEARGQRGLLGERTLRAFLRSMTGKSAEEI
                     AAGIEHLALEWQDGDPRDDVAVVVLQAPRRPA"
BASE COUNT         1535 a         3164 c         3132 g         1446 t
ORIGIN      
        1 gcgtggaggc ggtcgcgcgc ggcgccgcgc gcgaccgcct ccacgcacac gacgggcgcc
       61 attcccgcct gccggcggac ttgatcgcag tgctcggggg taccgacgag cttcgcgccc
      121 gacgcgaccg cgatctcggc cgcatcggcg agatggtcga agtggctgtg ccccgcgaag
      181 atgtattccg gcttgatcgc cgcaagcgtc tccggcgacg ccggcacgta accggagtac
      241 tcgccgcgcg gcacccaggc gtccaggagc acgacatggc ctccgatcgc ggcggcgaag
      301 ttcgtgaccc cgaaccagct caggatgacc cggtcgccgc gcaccctccc gtcgtcggcg
      361 acgttggccg caccgaacac ggcgcggcgg gccgccacga tcgattgacc ggctgcggcg
      421 cggtcgagcg gcacgacgac ggcgatgagg gccgcgacga gcgtgagcgc ggcggcggat
      481 cggcggttca tggcgtgctc cttggctcga gcgacggatg ccggacgatt tcgacgcgcg
      541 atggcgggat cccttcgccg ccggccggcc cgaccgccta caatctcgga ggccatgacc
      601 gacactcacg aagtcatcaa tcaggccgag ccgctgttgg gccacaactg gtatcgcagc
      661 gatcgcgccc tcgtcgaggc cgttcaccgc gaaggcgggg catgggccga agaccggatc
      721 gacgccctcg gcgccaaagc cgcctcgccc gaggcgacca cgtgggcgac gcaggcaaac
      781 gtctaccccc cggtgctccg cacgcacgat cgcgtcggca accggatcga cgaggtcgag
      841 ttccatcccg cgtggcacga gctgatgcgg gtgagcgtcg gcgccggcac gcacgcgctg
      901 ccgtggcgag agccgagagc gggggcgcac gtggcgcgcg cggccatgat gatggtctgg
      961 cagaccgatg ccggccacgg ctgtcccatc tcgatgacct acagcgtgat tcccgcgctc
     1021 cgcaagcagc cggagctcgc ggccgaatgg gaacccaggc tcctgtcgct cgactacgac
     1081 gagcgcttcg tcccggctcc ggacaagcgc ggcgccctcg ccggcatggc gatgaccgaa
     1141 aagcagggcg gcagcgacgt gcgggccaac accactcgtg ccgttccggt taacggcggc
     1201 ggcccgggcg ccgagtacga gatcaccggc cacaagtggt tctgctccgc tccgatgtgc
     1261 gacatgttcc tggtccttgc gcaggcgccc ggtggcctgt cgtgcttcct gctgcctcgc
     1321 tggcggccgg acggcacacg caacgcgttc cgcatccagc gtctcaagga caagctcggc
     1381 aaccggtcga acgcctcgag cgaggtcgag ttccacggcg catacgcccg catggtcggc
     1441 gaagaaggcc gcggcgtgcc gaccatcatc gagatggtca accacacgcg actcgactgc
     1501 gtactgggat ccaccgccgg catgcgctac ggcctgatga acgcgatcca ccacacgagc
     1561 cagcgcgccg ccttcggccg cgtcctgatc gagcagccgc tgatgcagaa cgtgctcgcg
     1621 gacctggccg tagaggccga ggccgcgacg gtgaccgcga tgcggctcgc gcgcgcctac
     1681 gacgcggccg ccgacgatga gcaggaggct ctgttcaagc gcgtcgcgac ggcaatcgcc
     1741 aagtactgga tatgcaagcg gcaaccggcg cacgtcggcg aggcgctcga atgcttcggc
     1801 gggaacgggt atgtcgagga ctccggcatg ccgcgtctgt accgcgaggc gccgctgaac
     1861 tcgatctggg aagggtccgg aaacgtgatc tgcctggacg tgcttcgggc atttgccaaa
     1921 acgccgggaa gcctcgacgc cttcttcgcg gaggtgtccc ttgccgaagg cgccgacgct
     1981 cgcttggacg cctacgtcaa ggatctgcgc aacgagctcg ccacccccgg cgacatggag
     2041 tcccgggccc gccggctcgt ggagcggctg gccttggccc tgcagggctc gcttctggtg
     2101 cggcactcgc ccccggagat cgcggacgca ttcagcgcct cgcggctggc cggtgactgg
     2161 ggccgggcgt tcgggacctt gccttcgcgc ctggacctgc cggtcatcgt cgagcgagcc
     2221 acccccattt gggattgacc tgctctctaa gcaagcccta cgattttttt tccggctaga
     2281 cggtttaagg cgttctgagc cgggtaactg gtcgggtgga ggtgtcggat ttcatggcaa
     2341 gggctcattc gatgaagact caaaagcttc ctcgaccgtc cgtgaagcgg acggagtccc
     2401 gtgctgcttc tcttgcgcgt gagggcatcc cccccaccga ggcccaacgc gccgcacgca
     2461 cgctccttgc cgaatccgac gcccgaacca agcgcgatcc cgccccccgg gatctgcgtg
     2521 acgacacggt agagcgccgg gatagcgacc tcgcgacgcc gccccccgac cttccgtcgt
     2581 gaggacgtaa ccggcaacgg cagtagatgg ctctccggcc gcctcggaga gccatctttc
     2641 ttttccgatg atgcgggcgc atgccgtccg cgtcccgggc cgctgaatgg ccgggcgttt
     2701 cgggggcagc gagccaggga aaccttggag tacagagcgg ccgccgggcg cggccgttga
     2761 acaaggagga cactatggct gcgattctcg tgcttgcgat aatcctggcg ttgttcggcg
     2821 gcggcttcgc gctcgagatt ctttggtacg tcgcgattgc cgcgctgatc ctgtgggtcg
     2881 tcgggttcct ggcccgcggc tccggcgcac gctggtaccg ctggtagcag cgacgagcgc
     2941 aacacgtcga aggccggccc gagggccggc cttccgtcgt gttggcctcg ggatgcggtg
     3001 cgcggggtag catgcgctca cgacgatcgc cggggggcga cgatggcggg acgcgacacg
     3061 cttcgcttgg ccgacctgtt ggccgcggtg tccgtggcga cggatctggg catgggccag
     3121 acaccggaga aggccattcg ttcctgcctg gtcgccacgc agttcgcggg gacgcttggg
     3181 atgcccggcc cggttgtcca cgacgtgtac ctgaccacgc tgctgcgaca cctcggatgt
     3241 acggccacgg cccacgagga ggcctacctg ttcgggggag acgaacgggc ctcgcgaccg
     3301 cccgcggaac gcaccgattt cggcagcgtt cgtgaggcgg cggcactgat gatggcaacg
     3361 ggacggggtg ccgggctcgg gcgcccccgc tatctggctc gagcgatacg agccggcaag
     3421 cagggcacgg acgcgatttt gcgagcgatc tgcgagatcg cttcgcgcct cgctgcacga
     3481 ctcgaactcg gaccgggcgt cgaggaagcg ctgtatcacg tgcacgaacg gtgggacggc
     3541 acggggagcc ccgcgaagct gcgcggtgac gacatcgcgc tccccgcccg cgtggcagag
     3601 atcgctacgc aggcaatcat cttcgacgcc gcggcgggcc ccgacgccgc agtcgccatg
     3661 gttcggcgtc gcgcgggcgg atggttcgac ccggcactcg ccgccgagtt cgagcgagtt
     3721 ggaccggcga tcctggccga ggtgcgcgca tcggatccct gggttgccgt cctcgacgcc
     3781 gagccggcac cgaatcgaac gatccgatca gccgatctcg aacggcttgc gcgcgcgttc
     3841 gcggacatgg tggatctgaa gtcgccctac accctcggtc attccagcga aacggcggca
     3901 ctcgcggttg ccgccgcacg cacgctcgcc atggacgacg acgactgccg tgacatcctg
     3961 catgcgacac tgctgcacga tctcggccgg accggcgttt cgaacggcgt gtgggacaag
     4021 cccgggccac tgtcgacatc ccagtgggaa caggtccggc tgcacgcgta tcacacggaa
     4081 cggatcatga tgcgatcggc ggcgctggca ccgctggccc cgatcgcggg agcgcatcat
     4141 gaacgccagg acggcagcgg ataccaccgg gcatcgaacg cgtcggcgat tcctccggcc
     4201 gcatgcctga tcgccgccgc tgatgccttc caggcgatga cgcaagaccg gcctcaccgt
     4261 cctgctttga gtcccgaggt ggcggcgaag gcgttggcgg cggacgcgac cgccggccgg
     4321 ctcgacccca cctgcgtccg ggcggtcatc gacgccgccg gacagtcccc gactcgagtc
     4381 cgcatcccat ggcccgcggg cttgaccgat cgcgaggtcg aggtgctgcg tctggtcgcc
     4441 ctcggatcgt ccaaccgcga gctggccgcc cgtctgttca tctcaccacg cacggcagaa
     4501 catcacgtgc agagcatcta cgccaaaatc ggcgcgtcga cccgcgcttc ggccgccctg
     4561 ttcgcgatgg agcacggcct gtatcgggcc ggcgacaaat aggtagacct accgattgcg
     4621 cgaaccccgc ggctgggcga tgctcgggtc aaaggaggtg ggacgcatgt acgcaaggat
     4681 caacacgttc gagggcgctc cagagggcat cgacgcgtcc gtggaacacg tccgcaccat
     4741 ggtgctaccg gtggcacgcg ggatcgatgg attcgccggg ctgctcgccc tcggggaccg
     4801 gaccaccgga aagaccatcg gcatcacgtt ctggagaagc ctcgaagcga tgcatgccag
     4861 cgaacaggcc gccgaccgcc ttcgcgacga ggtcgccgca gccagcaacg aacgagtcgt
     4921 ttcgatcgag caatacgagg ttctgctcga cgagaaagga ccgtgacatg gccgccatcg
     4981 acttcgtcat tcggacaacc acgtccccgg atacggtgtt cgcggccctg accgacttca
     5041 catcgcgccg gcctgcgctc tggccggcgc tggatccgga cgtctaccac gttgagatgc
     5101 tcggcgagac ccgggccctc gtacgcgaag ggcagcgctc gccccggctc tgggccctcg
     5161 aagagtacga ctggtccacg ccgggcacgg tcacctggac cgcgcgcgaa agcaacttct
     5221 gcgcgccggg cagcctcatg tctgcgacga tctctcccga aagcggcggc ggtagcgcgg
     5281 tcgcattccg gtggagccgc cgcggaatcg gcgtgaaggg acatctgatc gtcgcgatgg
     5341 tccgcgcaac gaagggaagg ccgttggtcg ccggcctgag caaagcactc gatgggctgg
     5401 ccgcgaagga gcagcaagcg cgttagcgcg ttggaatcgt ctcttcgccc gccgcttcac
     5461 gcagcatcgc gaaggttcga gacagcagcc gggacacgtg catctgcgag attccgagct
     5521 tgtcggcgat ctcgctctgc gacaggtcca acacgaaacg gtaatagagc agatgacgct
     5581 cgcgctcggg caggcgccgc agaagcggag cgacgtcggc ccactgctcc gtcatcgcca
     5641 tcgcggcgtc gtccgagccg agcgtctcgg ccacgccctg ctcatcttcg gcgacgggcg
     5701 cgtcgatcga tccgaccgac agcgcgtgca cggcgtcgag cgtctcgatg acgtcgtctt
     5761 cggaaaggcc ggatcgcacg gctatctcgg ggatcctggg cgaacggccg agctcttgac
     5821 cgagctcggc gatcacggtc ctgagccgaa gcgtctggtc ctgcaggcgg cgcggcacgc
     5881 gcatcgccca cccggtgtcg cggaagtggc gcttgagctc tccgacgatc gtgggcacgg
     5941 cgtaggtatc gaagcgcacc ccgcgctcga catcgaagcg gtccacggcc ttgagcagac
     6001 cgatgctcgc gacctgaacc aggtcgtcgt aggactcgcc gcggccggcg aaccgccgcg
     6061 cgagatgctc cgcgagcgga tagaaaaggc gcacgagctc ttcccgcaca ccgggttcgc
     6121 cccggcgcgg aaacagcaga gccgccttct cctcgcgtgt cgcgtcggcc ggcgcgttgt
     6181 cgtggtcgac gatctcttcg ctcatcacga aatgcgcttc gacattcgaa tcgccgcaga
     6241 gcccgcgtcc cgctccagca ggaccgagtc ggccagcgct tcgaggattc gccaggaaat
     6301 cgatgcgtcg ccgctgccgt tgccgtcatc gatcacggcg tcgatctgtg cgacgatctc
     6361 gaatgctcct cccgaccgtg ccagctccag acgcaacagc tccgcgtgcg aatgggccag
     6421 cagatgggtg catgcttcat ccacggcaag ccgaatgtct tcgatatcgt caagcgtcat
     6481 ctccgctttc gcggcgagcc ccgccgcgac cgaacgtgcg atgtgcacgt actccggcct
     6541 tgcgggcaat gtcaacgtta cgctccccat ggataacccc cggtcgtctg cttgctgata
     6601 ccccgtggct tcctcgtcaa aacaagcgac acggtgagaa ttcccgatgg tttggcacgc
     6661 gaccactacg ggtaccatag cccaagcaag cccaacaggg agggtcggat ggatcttgtc
     6721 gtcgattcga gaacggaagg aactgccgca gtcctgacgg tagggggcga aatcgacctc
     6781 ttcaccgcgc cgaagctccg tgaacggctc tcggggacga tcgatgaagg catggatcgc
     6841 gtcgtcgtcg acctcgaggg agtcggcttc atggactcaa ccggtctcgg gacgctggtg
     6901 ggaggcttga agcggatcaa ggaacgcgac ggctcgatgt cgctcgtgtg cacgaaccgg
     6961 tcggtcctcc ggatcctgtc gatcacgggt ctggatcggg tcttccccgt ctactcgtcg
     7021 gtcacggagg cgctgtcggc cgacgcacgg taaggcgccg gggggcgcac cgatacccgg
     7081 gggcacgggg catgaatcag gaagatcagc acgaccggct cgtcatcgag ctgccggccg
     7141 aagcagaggc cgtcggctcg gctcgcttgt tcgccacctc tgtcgcgcgg ctgttcggcg
     7201 tggacgacga agtcgtcgaa gatattcgcc tcgccatctc ggaggccgtg accgaacgcg
     7261 tgcaatcagc cgcatcggag gatgcgcccg tctgcgtctc gacgatcgtt cgtgagaaag
     7321 agctcgtgtt cgagatctcg ggcgggagcc gcacggccgg cgagaacggc gaactcggga
     7381 tcgccgtcgt gcacagcctg ttcccggacg ccgtcgtcga gcaggacccg cacgtgcttc
     7441 gcttcgtcgc gccgcgcgcc accgcgggct cgcaggctcc ttaggcgggg cgccgcggcg
     7501 cctgcagcac cacgaccgcg acatcatcgc gcggatcgcc gtcctgccat tcgagcgcca
     7561 aatgctcgat gcccgcggcg atttcctcgg cggactttcc cgtcatcgag cgaaggaacg
     7621 cccgcagcgt gcgctctccg agcagcccgc gctgtcctct cgcctcggtc acaccgtcgg
     7681 tgaacaggat gagcgagtct cccggcttga gccgcgcggt tcggtcgccg aggtccgggt
     7741 cttcgaacag gcccaggacc attccttcgc cggccgcctc ttcgagacgc cctccggcgc
     7801 gcagcaccag cggccgaggg tgtccacctc gggcgatcgc aagccggaca cccgtaggcg
     7861 acggacggac ccgtatgtag caggccgtgc agaagcggcc ggtgtcctgc tgaacgatgg
     7921 cacgattcaa cgtggtcagg accgcagaag gcttgcgcgc gctgatcgcg gccgcgcgaa
     7981 tcgtgtagcg ggccagcgcc gtcatcgcgg cggcttcggc ccctttgccg gacacatcgc
     8041 cgagcaccac ggcccagtcg ccgggatcgg tctggaagat gtcgtagaag tcgccgccga
     8101 cgtcgattcc gttcgtcgcc gacacataac gtgccgcgac gtcgaccccc tcgatcaccg
     8161 gaagaaccgg gggcagcagg ctctgctgaa ggacctgcgc gacccggctt cgctcggtga
     8221 acaggcgggc gttgtcgatg agaattccgg cgcgcgcggc gacctctgaa tacaggatcg
     8281 cgtcgtcttc gttcagccgt cgtccagact cggcggtgac cagggtgatc acgcccagaa
     8341 cacggtcacg agcggtcatc gggacgacga cgacggagtc gagcccgaga gaacgcagga
     8401 tgtcgaggtg ctcgtcgtca cgagcgacgg cttcgagcat ctcgtcgggc acgtgctcga
     8461 agatctgcgg cctgccggtt cgcatgaccg cccaagcgcc gacctcggac gccgggtccg
     8521 gtggatagcg tcgctgcagc tcgtgagcga gcgcgacctt gcggggatcg acgtgctcga
     8581 ccaccacacg acgcaggccg ccgttgtcga gcaggtcgat cgcgcaccaa tccaccagca
     8641 tggggacgaa ctcgcgcgcc agcacgcgca gccgctgatc gggatcgagc ggcgtttcgc
     8701 ccagcacgct cgagatacgc gccagaagcc ggaacacgtc gcgcgcacgg cgcacctcgg
     8761 cctcgatcgc gcgccgctcg gtcacgtccc ggaaggtcac gaccgacccg gcgagccggc
     8821 cgtcctccca cagaggggcg ctgatgtact cgaccgcgac ggaggttgcg tcctttcgcc
     8881 agaacacctc ggactcgatt cgatgcacgc taccgtcccg cgcggcttcg ttgatcggac
     8941 actcctcggc cgggtagggc gagccgtccg gagcggaatg atggatcgtc tcatgcaccg
     9001 gacgatcgat gagcccgccc cgctcccatc cgaggagctc ttccgcggca ggattgacga
     9061 aggtcacccg gccgtcgacg tcgacgcgaa tgatgccttc gcctgcggcg ttgaggagga
     9121 gctcgcggtc gcggatgagc cgcagcatct cgtccttcgc agctcgtgtg ggagccacgt
     9181 cgcgcagata cgacacgaag aaacgccccg acggcgtatc ggccgggcgc accgaggctg
     9241 ccacggtcgt cacgcttccg tccgccttga ggatggc
//
LOCUS       DATLFO010000356         6976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_8731, whole genome shotgun sequence.
ACCESSION   DATLFO010000356 DATLFO010000000
VERSION     DATLFO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8731"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..866
                     /locus_tag="VM841_14440"
     CDS             102..866
                     /locus_tag="VM841_14440"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HVL91425.1"
                     /translation="MEREPFDPADFERLRDSVPWISQVYAHVSLASTNEEAARLARDG
                     APDGTVVVADYQTAGRGRLDRSWTAEPGTSLLVSWIARPAITRDDFPLLTLATAAAAA
                     EAIERESGVTVDVKWPNDLLARGRKIAGILAEAVNGAAVIGLGLNVRQSEFPDEIAAT
                     ATSLLRERSRPVGRARLLGVILNAFEPFAAAPRSALESYRARCDTIGKEVRVERPDEE
                     PLTGRALRVADDGSLIVEAGGMETAVAAGDIVHLRV"
     gene            880..1248
                     /locus_tag="VM841_14445"
     CDS             880..1248
                     /locus_tag="VM841_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVL91426.1"
                     /translation="MITRISSAFYWTRNMDAAAAFYGDVLGLPLMRRHGDGFAQFDAG
                     GMPLGLHGPVDGVAPEGAGATVVLECDDLDATMKDLSAAGVVFEGEITETPNGARFTG
                     FRDPDGNLLQLIQPAAHPHP"
     gene            1248..1631
                     /locus_tag="VM841_14450"
     CDS             1248..1631
                     /locus_tag="VM841_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVL91427.1"
                     /translation="MAQPTVAGKPWEEFLAERPLGVIATTGAEGMPHAVPVEVIVKDG
                     RAYVWCKASSRKARNAARDGRAALVAYKMNDGVLLRGRVRLIVEGEPGYDSITKMFLT
                     KYERDETYGNDTLIEVTPHRVTTFS"
     gene            complement(1628..3049)
                     /locus_tag="VM841_14455"
     CDS             complement(1628..3049)
                     /locus_tag="VM841_14455"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HVL91428.1"
                     /translation="MLRVRPLIVLATAFAALAVGVAPATAAGSAFAVPPGFRTVAWEK
                     LGPGLDHVTMRGNYEAHNVHVARLKPDANYALRAVVAKQAISGGLETTSSMCRRTSCI
                     AGVNGDFFQVSGATSGQPIGGLSIFGQALRSPTPKHPQLLIGDDGRIRAGTDWWKGGI
                     TLADGRGITLDGVNVALRGGKISLFTAAFGKTTRNADKTTEIRIRIEQPRPPLQLGAP
                     ARIRFLDIRHIGDAPIPPDGAVIAGNGAGAQALDAVWWAIQNKHLSPLAEIEVSSPHT
                     DAIGSIGGSHVLLQDGRRVFQIYSRGLIQGRHPRTVVGWNKQGEVFLVTADGRQPRSA
                     EGMSLLQITNFMQQMGATDAINLDGGGSTTFVTRGNVINKPSDGRERGVATALVIVPV
                     APKPKAAAPKPAPSTPAPAVPVMPIPAIEPVAVPAAVTGVARTKGATSKDHAVAIAFM
                     MALGIGAIVVRSLFERVNRGYDD"
     gene            complement(3311..4855)
                     /locus_tag="VM841_14460"
     CDS             complement(3311..4855)
                     /locus_tag="VM841_14460"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVL91429.1"
                     /translation="MSDQLPKHTPEALHALVDQVLKGGAAKYHESAAKKGKLFVRERL
                     ALLLDKESFTEDALLANVLAGDLPADGVVTGVGRVDGRHVAIMANDSTVKAGSWGWRT
                     VEKILRIQEQAERLRIPLLYLVDSAGARITDQIQMFPGRRGAGRIFYNQVQMSGTIPQ
                     ICLLFGPSAAGGAYIPAFCDVVVMVDGNASMYLGSDRMVAQVTGEQTTLEEMGGAKMH
                     CSVSGCGDVLAASEQDAIAWGRRYLSYLPSSFEHAPPTEEPAAPAVTTDPAEIIEPNQ
                     NKPFDMHRLIEAIVDAGSWIEIKELFAKELLTGLARIEGRVVGILANQPKVKGGVLFV
                     DSADKAARFIWLCDAYNIPLLFLSDVPGFMVGSQVERQGIIRHGAKMISAVSDARVPK
                     LCVVIRKAYGAGLYAMAGPAFEPEATIALPTAMIAVMGPEAAVNAVYANKIAELPEDE
                     RPAFVEAKREEYRQDIDIYRLASEMIVDEVVEPKELRAAIARRFAAARTPTKPRPPKN
                     RPVTPV"
     gene            complement(4855..5628)
                     /locus_tag="VM841_14465"
     CDS             complement(4855..5628)
                     /locus_tag="VM841_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003342621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVL91430.1"
                     /translation="MSVVELDRGVVATITINRPEAANCLSRATLDELAAAIETLATDD
                     ECRAVVLTGEGEKAFCAGADLKERATMTDDQVRDFVRTIRDTATELSRLPQPVIAAIN
                     GAAFGGGCELALACDIRVIDEGAQIGLTETSLGIIPGGGGTQRLPRLIGPARAIELIV
                     TARRIDAAEACALGLANVVATSGTARKLAAEIADRIAANAPVAVRAAKEAAWRGLELP
                     LREGLELETDLYQRTLGTEDRIEGLTAFREKRPPVYKGR"
     gene            complement(5625..6557)
                     /locus_tag="VM841_14470"
     CDS             complement(5625..6557)
                     /locus_tag="VM841_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVL91431.1"
                     /translation="MSALPSRVTIVEVGPRDGLQNESATVPTDAKVAFIDRLSQSGLT
                     HIEATAFVSPKAIPQLADAEEVCNRIARTDGVVYSALVPNIKGYGRATEGAGLRDVAM
                     FLSASETHNRKNIGCSIDEAFGRYREVADAAERDGVRVRGYVSTAFGCPYEGNVPIEQ
                     VVEVARRMLDLGVQQVSLGDTTGMGNPAQVMQTVAALAEHTPHDTIALHLHDTRGTGL
                     ANALAGLQAGVTTFDASTGGLGGCPYAPGATGNVATDELVYMLEAMGVETGVDPEILL
                     QSAEIAGEAIGREPPSRYLKATRATQARERKENA"
     gene            complement(6568..>6976)
                     /locus_tag="VM841_14475"
     CDS             complement(6568..>6976)
                     /locus_tag="VM841_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HVL91432.1"
                     /translation="FALEAITDAMRLELAPWRIHVALIKPGAVATPIWDKGASDATAR
                     LDRMPPETTTYYETAIRRMRELAAHEAEIGIAPERVAAVVAHALTSLKPRTRYVLGPQ
                     AGLRVVMSKLISDRTRDKLVVKQTGLPGEGSAR"
BASE COUNT         1004 a         2367 c         2476 g         1129 t
ORIGIN      
        1 ccgccggggt cggttcgggc aaagacgatt ccacgtcggg ctccggatcc ggttcgggcg
       61 acgacgacta ggccgagcgc ggttcgatac gctgccgcct tgtggaacgc gaaccgttcg
      121 acccggccga cttcgaacgc ctccgcgata gcgtgccgtg gatttcgcag gtctacgcgc
      181 acgtctcgct ggcgtccacc aatgaggagg ccgcgcggct cgcacgcgac ggcgcacccg
      241 acgggacggt ggtcgtcgcg gattatcaga ccgcgggccg cggccggctc gaccggtcgt
      301 ggacggccga gccgggcacg tcgctgctcg tgtcctggat cgcgcggccg gcgatcacgc
      361 gcgacgactt tccgttgctg acgctggcga ccgccgccgc cgcggccgag gcgatcgaac
      421 gcgagtccgg cgtcaccgtc gacgtgaagt ggccgaacga ccttctggcc cgtggccgca
      481 agatcgccgg catcctggcc gaagcggtca acggcgcggc cgtgatcggg ctcggcctca
      541 acgtgcggca gagcgagttc cccgacgaga tcgctgcgac cgcgaccagc ctgctgcgcg
      601 agcgctcacg gcccgtcggc cgcgcccggc tgctcggcgt gatcctgaac gccttcgagc
      661 cgttcgccgc cgcgccccgt agcgcgctcg agtcctatcg cgcgcgctgc gacaccatcg
      721 gcaaggaggt gcgcgtcgag cggcccgacg aagagccgct cacgggccgg gcgttgcgcg
      781 tcgccgacga cggctcgctg atcgtcgagg ccggcggcat ggagacggcc gtggccgccg
      841 gcgacatcgt gcatctgcgc gtttgataga ttcgcgggca tgatcacgcg gatctcgagc
      901 gccttctact ggacgcggaa catggatgcg gccgccgcct tctacggtga cgtgctgggc
      961 ttgccgctga tgcggcgcca cggcgacggc ttcgcgcagt tcgatgcggg tggcatgccg
     1021 ctgggtctgc acgggccggt ggacggcgtc gccccggaag gggcgggcgc gaccgtggtg
     1081 ctcgagtgcg acgacctgga cgcgacgatg aaggaccttt cggccgccgg cgttgtgttc
     1141 gaaggcgaaa tcaccgagac gcccaacggc gcgcgcttca ccggctttcg cgatcccgac
     1201 ggcaacctgc tgcaactgat ccagccggcc gcgcacccac atccgtgatg gcgcagccga
     1261 cggtcgcggg caagccctgg gaagagtttc tggctgagcg tccgctcggc gtgatcgcca
     1321 cgaccggggc cgaagggatg ccgcacgcgg tgcccgtcga ggtgatcgtc aaagacggcc
     1381 gcgcgtacgt gtggtgcaag gcatcgtcac gcaaggcgcg caacgccgct cgcgacggcc
     1441 gcgccgcgct ggtcgcctac aagatgaacg acggggtgct gctgcgcggc cgggtccggc
     1501 tgatcgtcga aggcgagccg ggctacgact cgatcacgaa gatgttcctg accaagtacg
     1561 agcgggacga gacctacggc aacgacactc tcatcgaggt cacgccgcat cgcgtaacga
     1621 cgttttccta gtcgtcgtat ccgcggttga cccgctcgaa gagcgagcgc accacgatcg
     1681 ctccgatgcc cagcgccatc atgaacgcga tcgcgaccgc gtgatccttc gatgtcgcgc
     1741 ccttagtgcg ggccacgccg gtgacggccg cggggaccgc cacgggctcg atcgccggga
     1801 tcggcatgac cggcaccgcc ggggccggcg tggaaggcgc aggcttgggg gccgcggcct
     1861 tcggcttggg ggcgaccggc acgatcacca acgccgtcgc cacgccgcgc tcgcggccgt
     1921 cgctgggctt gttgatgaca ttgccgcggg tcacgaatgt cgtcgagcca cccccgtcga
     1981 ggttgattgc atcggtggcg cccatctgct gcatgaagtt ggtgatctgt agcagcgaca
     2041 tgccttcggc cgagcgcggt tggcgcccgt ccgcggtcac gaggaacacc tcgccctgct
     2101 tgttccatcc gacgaccgtg cggggatgac ggccctggat caagccgcgg gagtagatct
     2161 ggaagacgcg gcgtccgtcc tggaggagca cgtgcgagcc gccgatgctg ccgatcgcgt
     2221 cggtgtgcgg cgagctgacc tcgatctcgg cgagcggcga cagatgcttg ttctggatgg
     2281 cccaccacac cgcgtcgagc gcctgggcgc cggcgccgtt gccggcgatc accgcgccgt
     2341 cgggcgggat cggcgcgtcg ccgatatggc ggatgtccag gaaccggatc cgcgcgggcg
     2401 cgccgagctg aagcggcggt cgcggctgct cgatccggat ccggatctcg gtggtcttgt
     2461 ccgcgttgcg cgtggttttg ccgaaggcgg cggtgaacag cgagatcttg ccgccgcgca
     2521 gcgcgacgtt gacgccgtcg agcgtgatgc cgcggccgtc ggccagcgtg atgccgccct
     2581 tccaccagtc ggtgcccgcc cggatacggc cgtcgtcgcc gatcagaagc tgcggatgct
     2641 tgggcgtcgg agagcgcagg gcctggccga aaatcgacag cccgccgatg ggctgcccgc
     2701 tcgtcgctcc ggacacctgg aagaagtcac cgttcacgcc ggcgatgcac gaggtccggc
     2761 ggcacatcga ggaggtcgtc tcgagcccgc cgctgatggc ttgcttcgcc acgaccgcgc
     2821 gcagcgcgta attcgcgtcg ggcttcaggc gggcgacgtg gacgttgtgg gcctcgtagt
     2881 tgccccgcat ggtcacgtgg tcgagccccg gcccgagctt ttcccaggcc accgtgcgaa
     2941 accccggagg aacggcgaac gcggaaccgg ccgcggtcgc cggcgcgacg ccgacagcga
     3001 gcgcggcgaa agccgtggcg aggacgatca gcgggcgtac acgaagcatc agcaatcttc
     3061 cctggtcaac gagacggaca cgtgggagga cacgcgagga cgcggcaagc cgcaccgttg
     3121 cggcgaaagg gtctgagcgt ctcgggaggc cccccaaccg agcgtgcaca ttgtaggaaa
     3181 tccggctcgc ttcgggccag gagcggatga tgaaatcttc gataaaagtg acgcttatcg
     3241 cagagtgtcg ggcaaccgtt acgggttcga cgcggaaccg taacgtggcc gcccggatcc
     3301 gggcccgaag ctagacgggc gtgacgggcc gattcttcgg cgggcgcggt ttcgtcggcg
     3361 tccgcgcggc ggcgaaacgg cgcgcgatcg cggcccggag ctccttgggc tcgacgacct
     3421 cgtccacgat catctcgctg gccagccggt agatgtcgat gtcctgccgg tactcttcgc
     3481 gcttggcctc gacgaacgcg ggacgctcgt cctcgggcag ttcggcgatc ttgttcgcgt
     3541 agaccgcgtt gaccgccgcc tcgggtccca tgaccgcgat catcgccgtc ggcagcgcga
     3601 tggtcgcctc tggctcgaag gccggtcccg ccatcgcgta cagccccgcc ccgtaggcct
     3661 tgcggatcac gacgcatagc ttcggcacac gagcgtcgct gaccgccgag atcatcttcg
     3721 cgccatggcg gatgatgccc tgacgctcga cctggcttcc taccataaag ccgggcacgt
     3781 cggacaagaa caggagagga atgttgtacg cgtcgcatag ccagatgaac cgggccgcct
     3841 tgtcggctga atcgacgaac agcaccccgc ccttgacctt gggctggttg gccaggatcc
     3901 cgaccacgcg gccttcgatg cgagcaagcc cggtcagcag ctccttggcg aacagctctt
     3961 tgatctcgat ccacgacccg gcatcgacga tcgcctcgat cagccggtgc atgtcgaacg
     4021 gcttgttctg gttgggctcg atgatctcgg ccgggtcggt ggtcacggcc ggtgccgccg
     4081 gctcctccgt tggcggggcg tgctcgaagc tcgagggaag gtacgacagg tagcgccgcc
     4141 cccacgcgat cgcatcctgc tcgctcgcgg cgagcacgtc gccgcagccg ctgacgctgc
     4201 agtgcatctt ggcgccgccc atctcttcca gcgtggtctg ctcgccggtg acttgcgcga
     4261 ccatgcggtc cgagcccaga tacatcgacg cgttgccgtc caccatcacg accacgtcgc
     4321 agaaggcggg gatgtatgcg ccaccggccg cgctggggcc gaagagcagg cagatctgcg
     4381 ggatggtgcc cgacatctgg acctggttgt agaagatgcg gcccgcgccg cggcggccgg
     4441 ggaacatctg gatctggtcg gtgatgcgcg cgcccgcgct atcgaccaga tacagcagcg
     4501 ggatccgaag ccgttcggct tgctcttgga tgcgaaggat cttctcgacc gtgcgccagc
     4561 cccacgagcc ggccttgacc gtcgagtcgt tggccatgat cgccacatgc cggccgtcga
     4621 cccgtccgac gccggtgacc acgccgtcgg ccggcagatc gccggcgaga acgttcgcca
     4681 gcagcgcgtc ctcggtgaag gactccttgt ccagcagcag cgccagccgt tcacgcacga
     4741 acagctttcc cttcttggcc gcggactcgt gatacttggc cgcgccgccc ttcaagacct
     4801 ggtcgacgag cgcatgaagc gcttcgggcg tgtgcttggg caactggtcg ctcatcatcg
     4861 tcctttgtag acgggcgggc gcttctctcg gaaggcggtg aggccttcga tccggtcttc
     4921 ggttccgagg gtgcgctgat acagatcggt ctccagctcg agcccctcgc gcagcggaag
     4981 ctccaggccg cgccaggccg cttcttttgc ggcgcggacc gcgaccggcg cgttcgcggc
     5041 gatgcggtcg gcgatctcgg ccgccagctt ccgcgccgtg ccggacgtcg cgacgacgtt
     5101 ggccaggccg agcgcgcatg cctcggccgc gtcgatgcgg cgcgcggtca cgatcagctc
     5161 gatcgcgcgc gcagggccga tcaggcgcgg cagccgctgc gtgccgccgc cgccgggaat
     5221 gatgcccagg ctggtctcgg tcaggccgat ctgcgcgcct tcgtcgatga cgcggatgtc
     5281 gcaggcaagc gccagctcgc agcccccgcc gaacgcggcg ccgttgattg cggcgatgac
     5341 gggctgcggt agccgcgaga gctcggtcgc ggtgtcgcgg atcgtgcgca cgaagtcgcg
     5401 gacctgatcg tcggtcatgg tggcgcgctc tttcaggtcc gcgcccgcgc agaaggcctt
     5461 ctcgccctcg ccggtcagca cgaccgcgcg gcactcgtcg tcggtggcca gcgtctcgat
     5521 cgcggcggca agctcgtcga gcgtcgcgcg cgacaggcag ttcgcggcct cgggacggtt
     5581 gatggtgatc gtggccacga cgccgcggtc gagttcgacg acgctcatgc gttctccttg
     5641 cgttcgcgtg cctgcgtggc gcgggtggcc ttgaggtagc ggctcggcgg ttcgcgcccg
     5701 atcgcctcgc cggcgatctc ggcggattgc agcaggatct cggggtccac gccggtctcg
     5761 acgcccatcg cctcgagcat gtagacgagc tcgtcggtcg cgacgttgcc ggtcgcaccc
     5821 ggcgcgtacg ggcaaccgcc cagcccgccg gtggaagcgt cgaaggtcgt cacgcccgcc
     5881 tgcagcccgg cgagcgcgtt ggccaggccg gtgccgcgcg tgtcgtgcag gtgcagcgcg
     5941 atcgtgtcgt gcggggtgtg ctcggccagc gccgcgacgg tctgcatcac ctgcgcgggg
     6001 tttcccatgc cggtcgtgtc gcccagcgag acctgctgga cgcccaggtc gagcatccgt
     6061 cgcgcgacct cgacgacctg ctcgatgggc acgttgcctt cgtaggggca gccgaacgcg
     6121 gtggacacgt agccgcggac gcgtacgccg tcacgctcgg ccgcgtcggc gacctcgcga
     6181 tagcgaccga acgcctcgtc gatgctgcag ccgatgttct tgcggttgtg tgtctcggag
     6241 gccgacagga acatcgcgac gtcgcgcagg cccgcgcctt cggtcgcgcg gccgtagcct
     6301 ttgatgttgg ggaccagcgc cgaatagacg acgccgtcgg tgcgcgcgat ccgattgcag
     6361 acctcttcgg cgtcggcgag ctgcgggatc gccttcggcg agacgaacgc ggtggcctcg
     6421 atgtgcgtca ggcccgactg cgagagccgg tcgatgaagg caaccttcgc gtcggtcggc
     6481 acggtcgcgg actcgttctg cagtccgtcg cgcggaccga cctcgacgat ggtgacacgg
     6541 ctcggaaggg cgctcatggc ccgaatgcta ccgtgcgctt ccttcgccgg gcagtccggt
     6601 ctgcttgacg accagcttgt cgcgcgtccg gtccgagatc agcttcgaca tcacgacccg
     6661 cagccccgct tgagggccga gcacgtaccg ggttctcggc ttgagcgagg tcagcgcgtg
     6721 cgcgacgacg gcggcgaccc tctcgggggc gatcccgatt tcggcctcgt gtgcggcgag
     6781 ctcgcgcatg cggcggattg cggtctcgta gtacgtggtg gtctcgggcg gcatccggtc
     6841 caggcgtgcg gtcgcgtcgc tcgcgccctt gtcccagatc ggggtggcga ccgcgcccgg
     6901 cttgatcagg gcgacgtgga tgcgccaggg cgcgagctcg aggcgcatcg cgtcggtgat
     6961 cgcctcgagc gcaaac
//
LOCUS       DATLFO010000357        19549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_138074, whole genome shotgun sequence.
ACCESSION   DATLFO010000357 DATLFO010000000
VERSION     DATLFO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138074"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VM841_14480"
     CDS             <1..1122
                     /locus_tag="VM841_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL91433.1"
                     /translation="ASGVSLFEDWVRDLNGVLGAVGAERPSLLASTDAGPMAMLFAAA
                     YPDRIASLILTNTGARVTPAPDYPYGIPASLTDSFGALLSKEWGREHGSFPDVAWPTK
                     AHDPDFRRWVARLQRAATTPRGAASLFRQELDSDVRRALPLIQAPTLLVSSNFDLVPV
                     ANLEYLAEHISNSRLLKLEGKGAFGWLDDADRYLAAIEEHVTGRHAPVEPDRVLATIM
                     FTDIVGSTERASRLGDKKWRELLDRHDQIAQRHIGRFGGRLVKSTGDGVLAVFDGPAR
                     AVRCSAALAEDLRREQVEIRVGLHAGEVERRGEDVGGIAVHIAARVMALARPNEILAS
                     ATVKGLVIGSGLAFQDRGDHILSGLPDTWSLYAVMADAD"
     gene            1277..2818
                     /locus_tag="VM841_14485"
     CDS             1277..2818
                     /locus_tag="VM841_14485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HVL91434.1"
                     /translation="MSDRVRILDTTLRDGEQSPGINLNVKEKLEIAEQLARLHVDVIE
                     AGFPRTSQGDFDGVRAIADTIKGPAIAALARCVDEDIDRAWDALRVNAKPRIHVFLST
                     SDIHRKYMLNATEDEIVAQAVAGVQRAKGYTDDVEFSPQDATRTEPEFLFRIVEAVIE
                     AGATTVNVPDTVGYAIPYDFGEMLRECYKAVPRLAEITLSVHCHNDLGLAVSNSIEAI
                     RAGARQVEVAVNGIGERAGNCSLEEVVMAIATRRDLLNVETGINTRELARTSRLVSLL
                     TGYNVQRNKAIVGENAFAHESGIHQHGVLQDRLTYEIIKAEDIGVEGGKIVLGKHSGR
                     HAFSKQLEEMGFQLGKDELDRAFTRFKELVDRKIQITDKDLEAIIADEIQTADEIYRL
                     ESLQVAGGTHLAPTATVRVQRDGASLQESAMGDGMVDAVFGAVMRATGVAATLHSFNI
                     AAVTEGAEALGDVSVQLEIEGERYSGRGLATDIVEASARAFLNALNRAARHADRGPRS
                     IEPTP"
     gene            complement(3003..3827)
                     /locus_tag="VM841_14490"
     CDS             complement(3003..3827)
                     /locus_tag="VM841_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91435.1"
                     /translation="MEPLVTRIDHVVIPDEDPDDLFTLFTEGLGLPGLWPVSDVGPFR
                     SAGVAAGNCWIEIVAGAESLTPLYRPSLPAVVRGIALDAGGTCSDAAESLHAAGVPVG
                     PVTMFEGRRPDGVHGPLVTIAALPELVADAGIAYLCEMAAPVSAGRVADRTEAAAARS
                     ETVGIRRIAEIGIGARDIGAVSRAWSRLASPDGEGRWQLGGRPGERPGERLDGPAVHV
                     TESPIDGITGITFAVVALDRAVARLRARGLLGALRSSGAGVNHAAAHGLDLWLVEA"
     gene            complement(3875..8026)
                     /locus_tag="VM841_14495"
     CDS             complement(3875..8026)
                     /locus_tag="VM841_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVL91436.1"
                     /translation="MGDPITSVAGGRYVFSGFLGEGGRKKVYLGRDTRLERDVAIALI
                     ETAGLDEDGRERIHREARAMATLGSHNNIVDIYDVGEEAGHTYIISEYMAGGSIVDLI
                     ASRDGRLPIDDAIALTRQVCSALAYAHGHGIVHRDLKPQNVWIGADGQARLGDFGLAV
                     RRTEARLTSDGMLVGTVAYLPPEQATGREPDARSDLYSLGAMLYEMVTGRPPFPGDDA
                     VAVIGQHLNVAPVAPSWHNASVPPGLDILILELLAKDPAERPASATEVAARLDQVEQE
                     PSATAAPSAAVGRGPVRSRFVGREHERASLTGAVDAAGAGHGSLLFLVGEPGIGKTRL
                     SEEASAYARARGARVLVGHCYEAEASMPYIPWVEALRDYSASRSDEALRDELGEGAAD
                     VAKLVSEIRRRIPPLPASPQAEPEVERYRLFESATSFLVNAAREAPILLVLEDLHWAD
                     RPSLLLLQHLARRLSGSRIAVIGTYRDVELDRRHPLADVLAVLRRERLAQRIILRGLS
                     ADEIRVWLEATAQHRLGTGGALLAGALQSETEGNPFFIEEIVRHLLETERIYFRDGRW
                     RYDGAVEDLGIPEGVREVIGRRLSRLSEECNKVLSHASVIGREFSFDVVGAMSGVDED
                     GMLSAIEEALAAQLVHEVSGKTVPTYGFSHALVRQTLYEELSLPRKQRLHLRAAEAIE
                     TVFARNLAPHIASLAVHTRLAGAAADHDKALGYTLQAAQGAADLYAWEDAAAHLEAAV
                     EILADTDADPWQQARLLERLADVLYIGGSEHRKAVRSLEEAIRLYERAGATERAAQAH
                     VRIGFHLGTFPETMDLGRAVRHYRAAEPVLTAAGDSPALAYLYLGLGTASMWQYRFEE
                     AREPIERAVEIAGRLGNEGLLASATANLGWYQLTFGQVAEGLANVERAWQRADASGHV
                     FGAFLATWVAGQYLFGPLGAPIEAKQWIERELARPRSADTASTRRILSRTLTGIRFTL
                     GEIDGSEILAPEFDEQDWISLLAARNLGEWDLVETLAATVAERGRASENTNMTWGGVV
                     NLAFAYRDQGRPAEGAALLQASSHLLANGSMLDAYWLSVLALCLAQAGDLEAASDALG
                     RAKAIFDTDEDLRGSPGIALWAEAAMQSARGDVAHDAFSAAVSLLQRHGLARQEADVL
                     IDWGRACLAAGDHRGAIEHLGEALDILSRMGAPMARLEPVLADKLRAQGVDPASVQTS
                     VLALADSIAGQRPASDRSEFAIMFSDIEGSTVLTERLGDDRWLEVLRRHNELVRTAVA
                     AHGGTEVKAQGDGFMITFPDCPSAVACAIAVQQSFQEEAGPVKIRVRIGIHAGQVIEE
                     EGDVFGRNVILAARIADHASGGEIVVSDRVRERAGSTAVSRARSLRLKGIATPVSVCS
                     VVWRD"
     gene            complement(8079..8660)
                     /gene="leuD"
                     /locus_tag="VM841_14500"
     CDS             complement(8079..8660)
                     /gene="leuD"
                     /locus_tag="VM841_14500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HVL91437.1"
                     /translation="MEPVTTISGAVTVLDRADVDTDQIIPKQFLKRIERTGFGEFLFY
                     DWVRSGEVSLAPNPILATGRNFGCGSSREHAVWAIQQFGFAAVVAPSFSDIFWSNATK
                     NGLLPVALDEADVRTLMEAGRARIDLASQTVSFADRTATFDIDPEIKRRLLGGLDDIA
                     ITLTHDADIADFEAQASHPAPSTAGLERGRDAS"
     gene            complement(8661..10052)
                     /gene="leuC"
                     /locus_tag="VM841_14505"
     CDS             complement(8661..10052)
                     /gene="leuC"
                     /locus_tag="VM841_14505"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HVL91438.1"
                     /translation="MAKTLFDKIWQSHLVRDDLIYIDLHLVHEVTSAQAFDGLRLNGR
                     RVRRPDRTIATADHNVPTDGTPRASLIADPLSRVQVETLERNCAEFGIPVYSLGSDRR
                     GIVHVIGPELGLTQPGLTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQRRP
                     KTMRIRYDGTPGFGVTAKDLILATIGRLGTAGCTGYVVEYAGPAIEALSMENRMTVCN
                     MTIEGGGRAGMIAPDDATFAYLEGRDGAPKGSEWGDAVAYWRTLPTEPDARFDRHEVL
                     DASAVSPMVTWGTTPAMVTGVGDRVPDPAAFDSEADREAAERALAYMGLEPGTPITEI
                     GLDRVFIGSCTNSRIEDLRAAARIVDGRRIAPGIRAMVVPGSVAVKARAEAEGLDAIF
                     SSAGFEWRDAGCSMCLGMNPDTLSPQERCASTSNRNFEGRQGAGGRTHLVSPEMAAAA
                     AIEGRFVDIREWR"
     gene            complement(10072..10656)
                     /locus_tag="VM841_14510"
     CDS             complement(10072..10656)
                     /locus_tag="VM841_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVL91439.1"
                     /translation="MSLAAKSPVEPSAQVGRRVRALREARGMSLRDLAGRSGVSAPML
                     SQVERGATSPTLAVASKIAAGLDLSLSQLLRLEEGDHVVVSRRSERRAVRMSGHRYES
                     VTPDLPGQRASVTVHWLKPRARTGGNPLHDAGSRETLLVHAGTIALVLDGETYALEEG
                     DAITFDADLPHHIENPGRTEAAFAAVVTAGLRTT"
     gene            10786..11586
                     /locus_tag="VM841_14515"
     CDS             10786..11586
                     /locus_tag="VM841_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HVL91440.1"
                     /translation="MAEPVVALLDQVWSSIEEMCASFTPADWSKQTDLPGWSVQDQLA
                     HLCGIESLLLGRPQPAALAQPWPPHVRNELGAMNEAQIEERRAWAPEDVLTEFLELTA
                     ERLKVLAELSPAEMDAEVTGPLGKGKLRDFLQIRVMDSFLHEQDMRRATGNPGSMDGE
                     AASFAVGRMNAALPYVVAKKAGVAEGASVAFEITGPAGFTGAVVVEGGRGRFEEGNAS
                     GATATLTMDAETFLMLTSGRIAPDRATVTVTGDDALAGRVLDSINVLF"
     gene            11586..12614
                     /locus_tag="VM841_14520"
     CDS             11586..12614
                     /locus_tag="VM841_14520"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HVL91441.1"
                     /translation="MPHRIGIVAGDGIGPEVVGEGLRALGAAGVVIERVDLDLGAERY
                     LRTGDVLPDDAVKAIRDCDAVLLGAVGDPRVPPGVLERGLLLRLRFDLDLYVNLRPVK
                     LIEGIDTPLAGKGPADIDMLVVRENTEGLYTGAGGAHLVGTAGEVATQESINTRRGAE
                     RVIRYAFEQAGRRRRKVTMVHKTNVLTYAGALWTRAFEAVAADHPDIETDYVHVDAAC
                     LYFVTQPERFDVVVTENLFGDILTDLGAAIAGGMGVAASGNIDPERRGPSIFEPVHGS
                     APDIANQGRANPLACILSVALMLDHLGEAEAAGRIEAAVASAVRDGGVKGRSTRELGD
                     AVVRLIAG"
     gene            12686..13609
                     /locus_tag="VM841_14525"
     CDS             12686..13609
                     /locus_tag="VM841_14525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HVL91442.1"
                     /translation="MPVTPTEKVWMDGTLVPWSEAKIHILTHSLHYGGGVFEGIRAYE
                     TKRGPAVFRLTEHVARLFRSAKIFMIDIPFSEDEIVEACKETVRASGLPSCYVRPIVY
                     MGYGEMGLNPLLAPINVAVVVWPWGAYLGDEGLARGVRMKISSWRRHDPNIMPPAAKS
                     TGGYVNSQLAKMEAIKAGYDEAIMLNPQGLVAEATGENIFIVVDGELITPPLAAGALA
                     GITRDAVMTIARDHGIAVRETELARTDLYLAEEMFVSGTAAEVCPVREVDDRTIGDGR
                     PGPITKKLQETFYAAVKGEVEQYQSWLEYVN"
     gene            13615..15201
                     /gene="cimA"
                     /locus_tag="VM841_14530"
     CDS             13615..15201
                     /gene="cimA"
                     /locus_tag="VM841_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HVL91443.1"
                     /translation="MPSPLVLYDTTLRDGAQREGISLTVEDKLKVASWIDRLGVAYIE
                     GGWPGSNPKDMEFFRRGREIGMRKAKLSVFGATRRANGHAGADQNLRDIVDTEAPVAC
                     IVGKASRAQVELALGTTVEENLAMIADTVAFLTGEGMEVFFDAEHFFDGYKQDAAVAL
                     DALKAAIGAGATTVVLCDTNGGTMPGEVREIVPAVIEAVAGAAIVGVHFHNDTDCAVA
                     NSITSIELGAGHVQGTINGIGERCGNANMLSIIANCTLKLGLPAVSGEQLSELAAASH
                     AVAEICNVSPDPHQPYVGASAFATKAGLHTSGLAKMAGAYEHVDPAAVGNGRRLLVSE
                     LAGRSTIVMKGRELGIDLESDPEIAAKVLNAVKDLEHVGYTFEAADASLELLIRRTTG
                     DEHEYFRLESFRVLMEKRASGDVGTEATVKIWCKGERHIATAEGNGPVNALDGALRLA
                     LTKFYPALADIELSDYKVRILDSHQHTAATTRVLLESTDGRKEWSTIGVSPNIIEASW
                     QALCDSLAYGLLHDEVEESS"
     gene            15198..15953
                     /locus_tag="VM841_14535"
     CDS             15198..15953
                     /locus_tag="VM841_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVL91444.1"
                     /translation="MKICRVSYGGGISFGVVEGDDVAEIDGPPIGELRFTGKRAPLAD
                     CRLLAPVLPSKIVAIGVNYRDHAEEMGHTLPQEPLMFMKPATAVIGTGEAIHKPAVAE
                     RMDYEGELVVVVGGLVRKADRAAAAEAILGYTVGNDVTARDLQAKDGQWTRAKGFDSF
                     APIGPWIETDIDPGSLQIESRLNGEVRQSSNTTNLIFDPVGLVSSVSNVMTLLPGDCI
                     FTGTPSGVGPMEPGDSIEIEIEGIGVLKNTVAG"
     gene            15950..17407
                     /gene="gltX"
                     /locus_tag="VM841_14540"
     CDS             15950..17407
                     /gene="gltX"
                     /locus_tag="VM841_14540"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVL91445.1"
                     /translation="MTSPVQNIRTRFAPAPSGSLHVGNGRTALFAWLFARRHEGTFVL
                     RVEDTDASRATEEHMHGVMDVLRWLGLDWDEGPDVGGPYGPYRQSERYDLYRERADAM
                     VASGTAYACYCTPEELDERRKAALKAGKTPGYDRRCLDLSDPQRAAFASEGRAPAIRF
                     MVPEGETAFEDLVRGEVRFDHASISDFIILRADRSPTYLLAAATDDMEMKMTHVVRGE
                     DLMTATPRQMMLFAAMGVADVPRYAHLPLIVGSDRQPLSKRHGVTAIEHYRDNGFLPE
                     ALINYLALLGWSLGDGVTEKFSRDELIKYFTLENVSRNPAAFDIPKLTAINGDYIREL
                     APEEFARRLAPYLEREGLAPDDSLLAEIVPLIQERMQLLTEAAPMIRFFFIDAVEPDE
                     QAAAWLVPEYATALERYVAVLEALPEWRTEAIKDAMFGVQEALGLKKKAAFMPVRAAV
                     TGAKISPPLFESLEVLGRERSLGRIRAGLERARAG"
     gene            17475..17547
                     /locus_tag="VM841_14545"
     tRNA            17475..17547
                     /locus_tag="VM841_14545"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17509..17511,aa:Gln,seq:ctg)
     gene            17570..17645
                     /locus_tag="VM841_14550"
     tRNA            17570..17645
                     /locus_tag="VM841_14550"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17604..17606,aa:Glu,seq:ctc)
     gene            17979..18449
                     /locus_tag="VM841_14555"
     CDS             17979..18449
                     /locus_tag="VM841_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91446.1"
                     /translation="MRMRFAAAGLAVVAAFGGGSAFAQHTPAYQPSQHCDGTTPGGNN
                     VPVKTKIDLAKPALCFNLHGGFKGAVWVDFATSSVVIDGDSTNHTTIRCADGYAGVRL
                     AGSNGPTILFSQGDNYDPGASPRDGGTNGHAPGSPHDPFDPTTEHNEIHNCFIG"
     gene            18497..>19549
                     /locus_tag="VM841_14560"
     CDS             18497..>19549
                     /locus_tag="VM841_14560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91447.1"
                     /translation="MNIRPMTEDDREPFVALIRQAFNQAPPFVARMRELPIDTYRVAT
                     ESGRIVAAAAVLPTGHFFGGTPVACAGVSAVVVAPHARGGRTAEILVGEMLKERRPVM
                     PLSSLYPATVPIYRRLGYEYGAIRCTYRVPLHRLTRFGDALPATPWDDGDLDEVATAH
                     RAWAAEHNGVMDRTPSYWERLCKQIGDDPVYRVLVREGRQVTGSLIYHQENGFHMDLV
                     AHDLFWRTPAAARSLITYVARHHSLGRDFVWTGPSTDPMLFHSAEYKIREKERWHLML
                     RLLDVSAAVVSRGYRSGTETAVSVGVQDELFPENSGPWRIAVSGGKATAEPASDAEIT
                     MGVGTLASIYSGLITTH"
BASE COUNT         3123 a         6702 c         6716 g         3008 t
ORIGIN      
        1 gcctccggag tgtctttgtt cgaggactgg gtgcgcgatc tgaacggcgt cctgggtgcc
       61 gtcggagcgg agcggccgtc cctgctggcc tcgacggacg cgggaccgat ggcgatgctc
      121 ttcgcggctg cgtatcccga ccggatcgcg tcgttgatct tgacgaatac cggcgcgcgt
      181 gtgacgcctg caccggatta cccctacggg attccggcct ccctcacgga ctcgttcggc
      241 gccttgctgt cgaaggagtg gggccgggag cacggatcgt tcccggatgt tgcgtggcct
      301 acgaaggctc acgatccgga tttccggcgg tgggtcgctc gcctgcaacg cgcggccacg
      361 acgccccgag gcgcggcgag tctgttccgt caagagctgg actccgacgt gcgccgtgcg
      421 ctgccgctta ttcaagcccc gacgctgctg gtcagctcca acttcgatct ggttccggtc
      481 gccaacctcg aatatctcgc cgagcacatc tcgaattccc gtctgttgaa gctggagggg
      541 aagggagcgt tcggctggct ggacgacgcc gatcgctatc ttgcggcgat cgaggagcac
      601 gtgaccggcc gacacgcgcc cgtcgagccc gaccgtgtgc tcgccacgat catgttcacc
      661 gacatcgtcg gatcgaccga gcgggcctcc cgtttggggg acaagaagtg gcgagagctg
      721 ctcgatcgac acgatcagat cgcgcaacga cacatcggcc ggttcggcgg gcgtctggtc
      781 aaatcgaccg gcgacggagt tctggcggtg ttcgacggtc ccgctcgagc cgtgcgttgc
      841 tccgccgcgc tcgccgaaga tctgcgccga gaacaggttg agattcgggt cggcctgcac
      901 gccggggagg tggagcggcg gggcgaggac gtcggaggga tcgccgtgca catcgcggcg
      961 cgcgtgatgg cactggcgcg accgaacgag atccttgcga gcgcgaccgt caaagggctg
     1021 gtgatcggct ccggacttgc cttccaggac cgcggcgacc acattctctc cggcctgccg
     1081 gacacctggt cgctgtatgc cgtcatggcc gatgccgatt gacccaccga cggcgcgacg
     1141 cttgcctcgg cggggccggt tatgcgatga tggcagagat gagtggcaat ctgcttcttc
     1201 tttgacggcg tacgcggaat cgcgtgcgtc ggcccttcgc atccgcggag ggttttttcg
     1261 ttgaaaggga cggatgatga gcgatcgggt acggatcctc gacacgacgt tgcgggacgg
     1321 cgagcagtcg cccggcatca acctgaacgt gaaagagaag ctcgagatcg ccgagcagct
     1381 cgctcgtctg cacgtggatg tcatcgaagc agggttcccg cggacctcgc agggtgactt
     1441 cgacggggtt cgcgcgatcg cggacacgat caagggtccg gcgatcgcgg cgctggcccg
     1501 atgcgtggac gaggacatcg accgggcctg ggacgcgctt cgcgtcaacg cgaagccgcg
     1561 catccacgtg tttctgtcca ccagcgacat ccaccgcaag tacatgctga acgcgaccga
     1621 ggacgagatc gtcgcgcagg cggtcgcggg cgtgcagcgc gcgaagggct ataccgacga
     1681 cgtcgagttc agcccgcagg acgcgacccg aaccgagccc gagttcctgt ttcgcatcgt
     1741 ggaggcggtg atcgaggccg gcgcgacgac cgtcaacgtt cccgacacgg tcggctacgc
     1801 gatcccgtac gacttcggcg agatgctccg cgagtgctac aaggcggtgc cgcgcctggc
     1861 cgagatcacg ctgagcgtgc actgccacaa cgacttgggc ctggccgttt cgaactcgat
     1921 cgaggcgatc cgagccggcg cgcgccaggt cgaggtcgcc gtcaacggca tcggcgaacg
     1981 ggcggggaac tgctcgctgg aagaggttgt gatggcgatc gccacgcgac gcgacctgct
     2041 caacgtcgaa accggcatca acacccgcga gctcgctcgc acctcccgcc tggtgtcgct
     2101 gctcaccggc tacaacgtgc agcgcaacaa ggccatcgtc ggcgagaacg ccttcgcgca
     2161 cgagtccggc atccatcagc acggcgtgct gcaggaccgg cttacctacg agatcatcaa
     2221 ggccgaggac atcggcgtcg aaggcggcaa gatcgtgctc ggcaagcact ccggccggca
     2281 cgcgttcagc aagcagctcg aagaaatggg attccagctc ggcaaggacg agctcgaccg
     2341 cgccttcacg cgcttcaagg agctcgtcga tcgcaagatc cagatcaccg acaaagatct
     2401 ggaagcgatc atcgccgacg agatccaaac cgccgacgag atctaccggc tcgaaagcct
     2461 tcaggtcgca ggcggcacgc atcttgctcc gaccgcgacg gtgcgcgtcc agcgcgacgg
     2521 agccagcttg caagagtccg cgatgggcga cggcatggtg gacgcggtgt tcggtgcggt
     2581 gatgcgcgcg accggcgtgg cggcgacgtt gcactcgttc aacatcgccg ccgtgaccga
     2641 aggtgccgag gcgctcggtg acgtcagcgt gcagcttgag atcgaaggcg agcgctacag
     2701 cgggcgcggc ctggcgaccg acatcgtcga agcgagcgcg cgggcatttc tcaacgcgct
     2761 caaccgagca gcccgccacg cggaccgcgg ccctcgctcc atcgagccga ctccgtagcg
     2821 ttgcgcgagc gagccgtcaa agcccggacc atgcggcgat gtggaagctc gcgcacagcc
     2881 cgacgtcgag gacgtcctgc ccgccgaggg gctctgagag aggatctcgc cgtcggcctt
     2941 gaggatccag atcggatcgg cggcgttctg cacgagacga cggatccggg ggtgaccggg
     3001 cctcacgcct cgacgagcca caggtcgagg ccgtgcgcgg ccgcgtggtt gacgccggcc
     3061 ccgctcgagc gcagcgcgcc cagcaggccg cgcgcgcgga gccgggcgac cgcgcggtcg
     3121 agcgcaacga ccgcaaaggt gattcccgtg atgccgtcga tcggcgattc ggtgacgtgc
     3181 acggccggtc cgtcgagccg ctccccgggc cgctccccgg gccggcctcc gagctgccac
     3241 ctgccctcgc cgtccggcga cgccagtcgc gaccaggccc ggctcaccgc cccgatgtcg
     3301 cgcgcgccga tgccgatctc ggcgatgcgg cggatcccaa ccgtctcgct ccgcgccgcc
     3361 gccgcctccg tgcgatccgc gacgcgaccg gccgagaccg gcgccgccat ctcgcacagg
     3421 taagcaatcc ccgcgtcggc gaccagctcg ggcagcgccg cgatcgtcac gagcggtccg
     3481 tggaccccgt ccggccggcg tccctcgaac atggtgaccg gtcccaccgg cacgcccgcg
     3541 gcgtgcaggg actcggccgc atcggagcag gttccgcctg cgtcgagcgc gatcccgcgc
     3601 acgaccgcgg gaagcgatgg gcgatacagc ggcgtcagcg actcggcgcc ggcgacgatc
     3661 tcgatccagc agttgccggc ggcgacaccg gcgcttcgga acgggcccac gtcactgacc
     3721 ggccacaacc cggggaggcc gagtccctcg gtgaaaagcg tgaacagatc atccggatct
     3781 tcgtccggaa tgaccacgtg atcgattcgt gtgacgagcg gctccatgac cccgggcagt
     3841 gtatcggccg ccgagatcgc caggcccgcc gctgctaatc acgccagacg acggaacaga
     3901 cggataccgg cgtcgcgatc cctttgagcc gaagcgaacg cgcgcgcgag acggccgtcg
     3961 atcccgcccg ctcccgcacc cggtccgaca ccacgatctc gccgcccgag gcgtggtcgg
     4021 cgatgcgagc ggcgaggatg acgttgcggc cgaacacatc gccttcctct tcgatcacct
     4081 gcccggcgtg aatcccgatg cggacgcgga tcttcaccgg cccggcttcc tcctgaaacg
     4141 attgctggac ggcgatcgcg cacgccaccg cggacggaca gtcggggaac gtgatcatga
     4201 atccgtcacc ctgtgccttg acctcggtac caccgtgggc cgccacggcc gtgcgcacga
     4261 gctcgttgtg acgtcgcaga acctcgagcc agcggtcgtc gccgagccgc tcggtgagaa
     4321 cggtcgaccc ttcgatgtcg ctgaacatga tcgcgaactc gctccggtcg gatgcgggcc
     4381 gctgaccggc gatggaatcg gcaagcgcca gcaccgaggt ctgcacggag gcgggatcga
     4441 cgccctgcgc gcgaagcttg tctgccagca cgggctcgag ccgggccatc ggcgccccca
     4501 ttcgcgacag gatgtccagc gcctcgccga gatgctcgat cgcaccgcga tgatcgccgg
     4561 cggcgaggca cgcgcggccc caatcgatga gcacgtccgc ttcctgccgc gccagtccgt
     4621 ggcgctgcag gagcgacacg gcggcggaga acgcgtcgtg cgcaacgtcc cctcgggcgg
     4681 actgcatcgc ggcttctgcc cagagcgcga tgccgggaga gccgcgcaga tcctcgtcgg
     4741 tatcgaagat cgccttcgcg cggccgagcg catcggatgc ggcctcgagg tcgccggcct
     4801 gggcgaggca cagcgcgagg acgctcagcc aataggcatc cagcatgctt ccgttcgcca
     4861 gcagatggga cgaggcttga aggagtgcgg cgccctcggc cggccggccc tggtcccggt
     4921 aggcgaacgc gagatttacc acgccgcccc aggtcatgtt ggtgttctcg cttgcccggc
     4981 ctcgctcggc caccgtcgcc gcgagcgtct cgaccaggtc ccactcgccg aggtttcgcg
     5041 cggcgagcag cgagatccag tcctgctcgt cgaactccgg agcgaggatc tcggatccgt
     5101 cgatctcccc gagcgtgaat cggattccgg tgagcgtccg cgaaaggatg cgccgcgtgc
     5161 tcgccgtgtc ggccgaccgc ggcctggcga gctcgcgctc gatccactgc ttcgcttcga
     5221 tcggcgcgcc gagggggccg aacagatact ggccggcgac ccaggtcgcc agaaacgccc
     5281 cgaagacgtg ccccgacgcg tcggcgcgct gccaggcgcg ctcgacattg gcgaggcctt
     5341 cggcaacctg gccgaaggtg agctgatacc accccaggtt cgcggtcgca ctcgcaagca
     5401 gcccctcgtt gccgagccgt ccggcgatct cgacggctcg ctcgatcggc tcgcgtgcct
     5461 cctcgaatcg atactgccac atcgaggcgg ttcccaggcc gagatacaga tatgcgagcg
     5521 ccggcgagtc gcccgcggcc gtcaagaccg gctcggccgc gcgataatgc ctgaccgcgc
     5581 ggccgagatc catggtctcg gggaaggttc cgagatggaa gccgatccgt acgtgcgcct
     5641 gcgcggcacg ctccgtcgcc cccgcgcgct cgtagagccg gatcgcttcc tcgagtgagc
     5701 ggacggcttt tcgatgttcg gagccgccga tgtagagcac gtcggccagc cgctccagca
     5761 gccgtgcctg ctgccagggg tcggcgtcgg tgtcggcgag gatctcgacc gcggcttcga
     5821 gatgggcggc ggcgtcctcc cacgcgtaca ggtccgccgc gccttgcgcc gcctgcagcg
     5881 tgtagccgag cgctttgtcg tgatcggcgg cggcgccggc cagccgcgta tgcacggcga
     5941 gcgacgcgat gtggggcgcc aggtttcgcg cgaacaccgt ttcgatcgcc tcggccgcgc
     6001 gaaggtgaag ccgctgcttg cggggaaggc tcagttcctc gtagagcgtc tggcgcacga
     6061 gcgcgtgaga gaacccgtat gtcggcacgg tcttgccgct gacctcgtga acgagctgcg
     6121 ccgccagggc ctcctcgatc gcggacagca tcccgtcctc gtccacgcct gacatcgcgc
     6181 cgacgacgtc gaaggagaac tcgcgtccga tcaccgatgc gtgggacagg accttgttgc
     6241 actcctcgct cagccgcgac aggcggcggc cgatgacctc gcgcacgcct tcggggatcc
     6301 cgagatcctc gacggcgccg tcgtatcgcc agcggccatc ccggaagtag atgcgctccg
     6361 tctcgagcag atgccgcacg atctcttcga tgaagaacgg attgccctcg gtttcgctct
     6421 gcagcgcgcc tgccagcagc gcgccacccg tgcccagccg gtgctgcgcc gtggcctcga
     6481 gccagacgcg gatctcgtcc gcggacaggc cccgcaggat gatgcgctgg gcgagccgct
     6541 cgcgtcgcag cacggccaag acgtccgcga gcgggtggcg gcggtcgagc tcgacatcgc
     6601 ggtaggtccc gataacggcg atccggcttc ccgacagacg ccgggcgaga tgctgaagca
     6661 gcagcagcga cggccgatcc gcccagtgaa ggtcttcaag caccagcagg atcggtgcct
     6721 cacgtgcggc gttgacgaga aaggacgtcg cggattcgaa cagccggtag cgctcgacct
     6781 ccggctcggc ctgcggcgat gccggcagcg gcgggatccg gcggcgaatc tccgagacca
     6841 gcttcgcgac gtcggcggcg ccctcgccga gctcgtcgcg aagcgcctcg tcggagcgcg
     6901 aggcgctgta gtcgcgcagc gcctcgaccc acgggatgta gggcatcgac gcctcggcct
     6961 cgtagcagtg gccgacgagc acgcgcgcgc cgcgagcgcg cgcgtacgcc gacgcttctt
     7021 cggaaagccg tgtcttaccg atgccgggct cgccgaccag gaacagcagc gagccgtgcc
     7081 cggcgccggc ggcgtccacc gcgccggtta gcgaggcgcg ctcgtgctcg cgcccgacga
     7141 accgcgaccg gaccggtcct cggcccacgg cggcggaggg ggccgccgtg gccgacggct
     7201 cctgctcgac ctggtcgagg cgcgccgcaa cctcggtggc cgacgccggc cgctcggcgg
     7261 ggtcctttgc gagcagctcc aggatcagta tgtcgaggcc gggcggcacc gatgcgttat
     7321 gccacgacgg cgccaccggt gcgacgttga gatgctggcc gatgacggcg acggcgtcgt
     7381 cgcccgggaa cggcgggcgt cccgtaacca tctcgtagag catggcgccc agcgagtaca
     7441 ggtcgctgcg tgcgtccggt tcgcgtccgg tcgcctgctc cggcggaaga taggcgaccg
     7501 ttccgacgag cattccgtcg gaggtcaggc gagcctcggt gcggcgcacg gccagaccga
     7561 agtctcccag gcgcgcttgt ccgtccgcgc cgatccatac gttttggggc ttgagatcgc
     7621 ggtgcacgat gccatggccg tgcgcgtaag ccagggccga gcagacctgg cgcgtgagcg
     7681 cgatcgcgtc gtcgatcggc aggcgtccgt ccctcgatgc gatcagatcc acgatcgagc
     7741 cgcctgccat gtactcgctg atgatgtagg tgtgaccggc ttcttcaccg acgtcgtaga
     7801 tgtccacgat gttgttgtgg gagccgagcg tggccatcgc gcgtgcctcg cggtgaatgc
     7861 gctcgcgtcc gtcctcgtcg agcccggcgg tctcgatcag cgcgatggcg acgtcccgct
     7921 cgagtcgcgt gtcgcggccg aggtaaacct tcttgcggcc gccttcgccg agaaatcccg
     7981 agaagacgta tcgtcctccg gcgacggatg tgatcggatc ccccatgccc gccattgttt
     8041 cgcgtggctg cgagcgcgtc caacttgcct tcggcccgct acgaggcgtc gcggccgcgc
     8101 tcgaggcccg cggtcgaggg cgccggatgc gacgcctggg cttcgaaatc cgcgatgtcg
     8161 gcgtcgtgcg tcagcgtgat cgcgatgtcg tcgagcccac cgagcagccg ccgcttgatc
     8221 tcgggatcga tatcgaaggt tgcggtgcga tcggcgaagg agaccgtctg cgatgccaga
     8281 tcgatcctgg ctcggccggc ttccatcagc gtgcgaacgt cggcttcgtc gagcgcgacc
     8341 ggcagcagcc cgttcttcgt ggcgttcgac cagaagatgt cgctaaacga cggcgcgacg
     8401 accgcggcga agccgaactg ctggatcgcc cagaccgcgt gctcgcggct ggagccgcat
     8461 ccgaagttgc gacctgtcgc gaggatcggg ttcggcgcga gcgagacctc gcccgagcga
     8521 acccagtcgt agaacaggaa ctcgccgaag ccggttcgct cgatgcgctt gaggaactgc
     8581 ttggggatga tctgatcggt gtccacgtcc gcgcggtcca agaccgtgac ggccccgctg
     8641 atcgtcgtga ccggctccat ctatctccac tccctgatgt ccacgaagcg gccttcgatg
     8701 gcggcggcgg cggccatctc gggtgagacc agatgcgtgc gtccgcccgc gccttgccgt
     8761 ccttcgaagt tgcggttcga ggtcgaggca cagcgctctt gcggcgagag cgtgtccggg
     8821 ttcatgccca ggcacatcga gcacccggcg tctcgccact cgaacccggc cgacgaaaag
     8881 atcgcgtcga gcccttccgc ctcggcccgg gctttgacgg caacgcttcc ggggacgacc
     8941 atcgcgcgaa tgcccggtgc gatccggcgg ccgtccacga ttcgggccgc ggcacgcaga
     9001 tcctcgatcc gcgagttcgt gcacgagccg atgaacacgc gatcgaggcc gatctccgtt
     9061 atcggcgttc cgggctcgag ccccatgtac gcgagcgccc gctcggccgc ctcgcgatcg
     9121 gcttcggaat cgaacgccgc cgggtccggg acgcgatcgc cgacgccggt caccatggcc
     9181 ggcgtggttc cccaggtgac catgggagag accgcgctcg cgtccaagac ctcgtggcga
     9241 tcgaatctcg cgtcgggttc ggtcgggagc gttcgccaat acgccaccgc atcgccccac
     9301 tccgagccct tcggcgcccc gtcccgtcct tcgaggtagg cgaaggtcgc gtcgtcgggt
     9361 gcgatcatcc cggcccggcc gccgccttcg atggtcatgt tgcacaccgt catgcggttc
     9421 tccatcgaca gcgcctcgat cgcgggaccg gcgtactcga ccacgtaccc ggtgcagccg
     9481 gcggtgccca gccggccgat cgtcgccagg atcagatcct tggccgtgac gccgaagccc
     9541 ggcgtgccgt cgtagcggat gcgcatggtc ttgggccgcc gctgcaccag cgtctgggtc
     9601 gcgagcacgt gctcgacctc gctggtgccg atgccgaacg cgagcgcgcc gaaggccccg
     9661 tgcgtcgagg tgtgcgaatc gccgcatacg atcgtcaggc ccggctgggt caggccgagc
     9721 tccgggccga tgacgtgaac gatgccgcgc cggtcgctgc cgagcgagta caccggaatc
     9781 ccgaactcgg cgcagttgcg ctcgagcgtc tcgacctgca cgcgcgagag cggatcggcg
     9841 atgagcgagg cacgaggtgt tccgtcggtc ggcacgttgt ggtcggccgt cgcgatggtg
     9901 cggtcgggtc gccggacgcg ccggccgttg agccgcagcc cgtcgaacgc ctgggccgag
     9961 gtcacctcgt gcaccagatg caggtcgatg tagatcaggt cgtcgcggac cagatgcgac
    10021 tgccagatct tgtcgaagag cgtctttgcc atgatcggaa tcctccgcgg atcacgtcgt
    10081 gcgaagcccg gcggtcacca cggccgcgaa cgccgcctcg gtgcggccgg gattttcgat
    10141 gtggtggggg agatcggcgt cgaaggtgat cgcgtcgcct tcctccagcg cataggtttc
    10201 gccgtcgagc accagagcga tggtgcccgc gtggacgagc agcgtctcgc ggctgccggc
    10261 atcgtggagc gggtttcctc cggtgcgggc gcgcggcttg agccagtgca cggtgacgct
    10321 ggcccgctgg ccgggcaggt ccggagtgac ggactcgtag cggtgcccgc tcattcggac
    10381 cgcgcgccgc tcgctgcgcc tggacacgac cacgtgatcg ccctcctcca gccggagcag
    10441 ctgcgacagg gacagatcga ggcccgcggc gatcttgctc gcaacggcaa gcgtcgggga
    10501 ggtcgccccg cgctcgacct gggacagcat cggggcgctc acgccgcttc ggcccgccag
    10561 gtcccgaagc gacatgcccc gcgcctcccg cagcgcacga acgcggcggc cgacctgggc
    10621 ggacggctcg accgggctct tcgcggcgag ggacacgacg acgtatgcta tcacgaacag
    10681 tcgtacgtga tcatcccttt cccgaagacg ggcttatcgc ctacactctc aggttcgcgg
    10741 gtcacggggg gatggcccga gagccagggg aggagtgctt tcaagatggc cgaacccgtc
    10801 gtggccttgc tcgatcaggt ctggtcctcg atcgaggaga tgtgcgcatc gttcacaccc
    10861 gctgactgga gcaagcagac cgacctgccg gggtggagcg ttcaggatca gctcgcccat
    10921 ctgtgcggca tcgagtcgct gctcctgggc cggcctcagc cggccgcgct ggcccagccg
    10981 tggccgccgc acgtgcgcaa cgagctcggg gccatgaacg aggcccagat cgaggagcgc
    11041 cgggcctggg ctcccgaaga cgtgctcacc gagttcctcg agctgacggc ggaacgcctc
    11101 aaggtcctgg ccgagctctc cccggccgag atggacgccg aggtgaccgg cccgctcggc
    11161 aaaggaaagc tgcgcgactt ccttcagatc cgcgtgatgg actcgttcct ccacgagcag
    11221 gacatgcgcc gggcgaccgg caacccgggt tcgatggacg gcgaggccgc gagcttcgcg
    11281 gtcggccgta tgaatgccgc cctgccgtac gtggtggcca agaaagccgg agtcgccgag
    11341 ggggcctccg tggcgttcga gatcacgggc ccggcaggct tcaccggagc cgtggtcgtc
    11401 gaaggcggcc gcggccggtt cgaggagggc aacgcttccg gcgccaccgc cacgcttacg
    11461 atggacgccg aaacgttcct gatgctgacc agcggccgca tcgcgcctga tcgcgcgacg
    11521 gtcaccgtga ccggcgacga cgcgctcgcc ggccgggttc tcgactccat caacgtcctg
    11581 ttctgatgcc ccatcgcatc ggcatcgtcg ccggagacgg catcggtccg gaggtcgtcg
    11641 gcgaggggct gcgcgccctc ggcgcggccg gcgtggtcat cgagcgcgtc gatctggatc
    11701 tgggcgccga gcgctacctg cggaccggcg acgtgcttcc cgacgatgcg gtcaaggcga
    11761 tccgcgactg cgacgcggtt ctgctcggcg cggtgggcga tccgcgagtc ccgccgggcg
    11821 tgctcgagcg cggcctgctg ctgcggctgc gcttcgatct ggacctgtac gtgaacctgc
    11881 ggccggtcaa gctgatcgag ggcatcgaca ccccgctcgc cggcaaggga cccgccgaca
    11941 tcgacatgct cgtggtgcgc gagaacaccg agggcctcta caccggcgcg ggcggcgctc
    12001 acctggtcgg gaccgcgggc gaggtcgcga cgcaggagtc gatcaacacc cgccgcggcg
    12061 cggagcgcgt gatccggtac gccttcgagc aggccgggcg tcgccggcgc aaggtcacca
    12121 tggtgcacaa gacgaacgtg ctcacctacg ccggcgcgct ctggacacgc gcgttcgaag
    12181 cggtcgcggc cgaccatccg gacatcgaga ccgactacgt gcacgtggat gccgcctgtt
    12241 tgtacttcgt gacccagccc gagcgcttcg acgtggtcgt gacggaaaac ctgttcgggg
    12301 acatcctgac cgatctggga gcggcgatcg ccggcggaat gggcgtcgcc gccagcggaa
    12361 atatcgatcc cgagcgacgc ggaccgagca tcttcgagcc cgtgcacgga tcggctcccg
    12421 atatcgcgaa tcagggccgt gccaacccgt tggcctgtat cctttcggta gccctgatgc
    12481 tcgaccacct gggcgaggcc gaggcggccg gccgtatcga agcggcggtg gcctcggcgg
    12541 ttcgcgacgg tggcgtcaag ggccgttcga cgcgcgagct cggagacgcg gtcgtccggc
    12601 tgatcgccgg atagccgccg accggtccgg cgcacgctcc accagagcgc ggacgcggac
    12661 cgataatttc agaacggagg acgccatgcc ggtgacgccc acggaaaagg tttggatgga
    12721 cgggacgctc gttccctgga gcgaggccaa gatccacatc ctgacgcatt cgctgcatta
    12781 cggcggcggc gtcttcgaag gaatccgcgc ctacgagacc aagcgcggtc ccgccgtctt
    12841 ccggctcacc gagcacgtcg cgcgcctgtt ccgctcggcc aagatcttca tgatcgacat
    12901 tcccttcagc gaggacgaga tcgtcgaggc ctgcaaggag accgtgcgtg cctcggggct
    12961 tccgtcctgc tacgtgcgtc cgatcgtcta catgggctac ggcgagatgg gcctgaaccc
    13021 gctgctcgcg ccgatcaacg tcgcggtcgt ggtgtggccg tggggcgcct acctgggcga
    13081 cgaggggctc gcccgcggcg tgcggatgaa aatctcgtcg tggcgccgtc acgaccccaa
    13141 catcatgccg cccgcggcca agagcaccgg cggctacgtc aactcgcagc tcgccaagat
    13201 ggaggcgatc aaggccggct acgacgaggc gatcatgctc aaccctcagg ggctggtcgc
    13261 cgaggcgacc ggcgagaaca tcttcatcgt cgtggacggc gagctgatta cgccgccgct
    13321 cgcggccggg gcgctcgcgg gcatcacccg cgacgcggtc atgacgatcg cgcgcgatca
    13381 cgggatcgcc gtgcgcgaga ccgagctcgc gcgcaccgac ctgtacctgg ccgaggagat
    13441 gttcgtgtcg ggcacggccg ccgaggtctg cccggtgcgc gaggtggacg accgcaccat
    13501 cggcgacggc cgtccgggtc cgatcaccaa gaagctccag gaaaccttct acgcggccgt
    13561 caagggcgag gtcgagcagt accagtcctg gctcgagtac gtgaattgag gccggtgcct
    13621 tcgccgctgg tcctgtacga cacgacgctg cgcgacggcg cgcagcgtga gggcatctcg
    13681 ctcacggtcg aggacaagct caaggtcgcc tcgtggatcg accggctcgg cgtcgcctac
    13741 atcgaaggcg gctggccggg ctcgaacccg aaggacatgg agttcttccg ccgcggccgc
    13801 gagatcggca tgcgcaaggc gaagctgtcg gtcttcggcg caacgcgccg cgcgaacggg
    13861 cacgccggcg ccgaccagaa cctgcgcgac atcgtcgaca ccgaagcgcc cgtcgcgtgc
    13921 atcgtcggca aggccagccg cgcgcaggtc gagctcgccc tgggaacgac ggtggaggag
    13981 aacctggcga tgatcgccga caccgtcgcc tttctgacgg gcgaaggcat ggaggtcttc
    14041 ttcgacgccg agcacttctt cgacggctac aagcaggatg ccgcggttgc gctggacgcg
    14101 ctcaaggccg cgatcggcgc gggcgcgacc accgtggtgc tgtgcgacac caacggcggc
    14161 acgatgccgg gcgaggtgcg cgagatcgtg cctgcggtga tcgaggcggt cgcgggcgcc
    14221 gcgatcgtcg gcgtgcactt ccacaacgac accgactgcg cggtcgcgaa ctcgatcacc
    14281 tcgatcgagc tgggggccgg gcacgtgcaa ggcacgataa acggcatcgg cgagcgctgc
    14341 ggcaacgcga acatgctcag catcatcgcc aactgcacgc tcaagctggg gctgcccgcg
    14401 gtcagcggcg agcagctctc cgagctcgcg gccgcgagcc acgccgtcgc cgagatctgc
    14461 aacgtcagcc ccgatcccca tcagccctac gtcggcgcga gcgcgttcgc cacgaaggcg
    14521 ggcctgcata ccagcggcct ggcgaagatg gccggcgcat atgagcacgt cgatcccgcc
    14581 gcggtgggca acggccggcg gctgctggtc agcgagctcg cgggccgctc cacgatcgtg
    14641 atgaaaggac gcgagctcgg catcgatctg gagtccgatc ccgagatcgc ggcgaaggtc
    14701 ctcaacgcag tcaaggacct cgagcacgtc ggctacacct tcgaggcggc cgacgcctcg
    14761 ctcgagctgc tgatccggcg gaccaccggc gacgagcacg agtacttccg gctggagtcg
    14821 ttccgcgtgc tgatggagaa gcgcgcgagc ggcgacgtcg gcaccgaggc aacggtcaag
    14881 atctggtgca agggcgaacg gcacatcgcc accgccgaag gaaacggtcc ggtcaacgcg
    14941 ctcgacggcg ccctgcgcct cgcgctgacg aagttctatc cggcgctggc cgacatcgag
    15001 ctcagcgact acaaggtccg catcctggac tcgcaccagc acaccgccgc gaccacgcgc
    15061 gtgctgctcg agtccaccga cggccgcaag gaatggtcca cgatcggagt gtccccgaac
    15121 atcatcgaag catcgtggca ggcgctctgc gactcgctcg catacgggct gctgcatgac
    15181 gaagtggagg aaagctcgtg aagatctgcc gggtgtccta cgggggcggt atctcgttcg
    15241 gcgtggtcga aggcgacgac gtcgccgaga tcgacggtcc gcccatcggc gagttgcggt
    15301 tcactggcaa gcgcgcgccg ctcgccgact gccgcctgct cgccccggtg cttccgtcca
    15361 agatcgtcgc catcggggtg aactaccgcg accacgccga agagatgggc cacacgcttc
    15421 cccaagagcc gctgatgttc atgaagcccg caaccgccgt gatcggaacc ggcgaggcga
    15481 tccacaaacc ggccgtcgcc gaacgcatgg actacgaagg cgagctggtc gtcgtcgtcg
    15541 gcggtctggt ccgcaaggcc gaccgtgccg cggccgccga ggcgatcctg ggctacaccg
    15601 tcggcaacga cgtcaccgcc cgggacctcc aggccaagga cggccagtgg acgcgcgcga
    15661 agggctttga ttcgttcgcg ccgatcggcc cgtggatcga gaccgacatc gatccgggct
    15721 cgctgcagat cgagtcgcgg ctcaacggcg aggtgcgcca gtcctcgaac actacgaacc
    15781 tgatcttcga ccccgtcggg ctcgtttcct ccgtcagcaa cgtcatgacc ctgcttcccg
    15841 gcgattgcat cttcaccggc acgccgtcgg gggtgggacc gatggagccg ggcgacagca
    15901 tcgagatcga gatcgaaggc atcggtgtgc taaagaacac ggtggccgga tgacctcgcc
    15961 cgtccagaac attcgcaccc gcttcgcgcc cgctccctcg ggatcgctgc acgtcggcaa
    16021 cggccggacc gcgctgttcg cctggctgtt cgcccgccgg cacgagggaa cgttcgtcct
    16081 gcgcgtcgag gacaccgacg cctcgcgggc gaccgaggag cacatgcacg gcgtcatgga
    16141 cgtgctgcgc tggctcggtt tggattggga cgaaggcccc gacgtcggcg gcccctacgg
    16201 cccgtaccgt cagtccgagc gatacgacct gtatcgcgag cgcgccgatg cgatggttgc
    16261 atccggcacg gcctacgcct gctactgcac gcccgaagag ctcgacgagc ggcgaaaggc
    16321 cgcgctgaag gcgggcaaga cgcccggcta cgaccgccgc tgcctcgacc tgagcgatcc
    16381 gcaacgcgcg gcattcgcgt ccgagggccg cgcgccggcg atccgcttca tggtccccga
    16441 gggcgagacc gccttcgagg atctggtgcg cggcgaggtg cgcttcgacc acgcgagcat
    16501 ctcggacttc atcatcctgc gggccgaccg ctcgccgacc tatctgctcg cggccgccac
    16561 cgacgacatg gagatgaaga tgacccatgt cgtacgcggc gaggatttga tgaccgccac
    16621 gccgcggcag atgatgctct tcgccgcgat gggcgtggcg gacgttccgc gctacgcgca
    16681 tctgccgctc atcgtcggat cggaccggca gccgctgtcg aagcggcacg gcgtgaccgc
    16741 gatcgagcac taccgcgaca acggcttcct gcccgaggcg ctgatcaact acctggcgct
    16801 cctgggctgg tcgctcggcg acggggtgac cgagaagttc tcacgcgacg aactgatcaa
    16861 gtacttcacg ctcgagaacg tctcgcgcaa ccccgcggca ttcgacatcc cgaagctgac
    16921 cgcgatcaac ggcgactaca tccgcgagct tgcgcccgag gagttcgccc gccgcctggc
    16981 tccgtatctg gagcgtgagg ggctggcccc ggacgattcg ctgctggccg agatcgtgcc
    17041 gctgatccag gagcgcatgc agctcctgac cgaggccgcg ccgatgattc ggttcttttt
    17101 catcgacgcg gtcgagccgg acgagcaggc ggccgcatgg ctcgtgcccg agtacgcgac
    17161 cgcactggag cgttacgttg ccgtcctcga ggcccttccc gagtggcgaa ccgaggcgat
    17221 caaggacgcg atgttcgggg tccaggaagc gctcggcctg aagaagaagg ccgcgttcat
    17281 gccggtgcgg gccgccgtga cgggagccaa gatctcgccg ccgctgttcg agtcgctcga
    17341 ggtgctcggc cgcgagcgct cgctcgggcg gattcgcgcc ggcctggagc gggcgcgggc
    17401 cggatgaaga gcctgcgtgt cgcggtgttc ggttgccgcg ccgcccccac cccgatagca
    17461 tcctggggtt tccttggggc gtagcgtaac ccggcagcgc gcctgattct ggttcaggta
    17521 gtcctggttc gaatccaggc gccccagcta ggagcaattc cgaggaaaag gccccttcgt
    17581 ctagcggcct aggacagcgg cctctcacgc cgcaaacacg ggttcgaatc ccgtaggggt
    17641 caccatcgaa aagcctccgc gaacgcgggg gctttctcgt tgtccgggct gaagaccgct
    17701 taaggctctg gtgggacggc gtgacgcgat ctcggcggtg tccgtgatgt cgggacgcga
    17761 tgcgcaaccg attccccgtc ccgcggctct tccgctttct cctccgaagg acggacggcg
    17821 attccggctc gcccgcaacc tccggtttgt gacggcgaac tcgcgaggga agacgcggcg
    17881 ggaggaagaa gattcacgca ggcgcctgga gggggtgccg cccccgccgg gccattggtc
    17941 cgcgcggggc ccgcacgcag gacgagggag agggtctcat gcgcatgcgt ttcgcagcgg
    18001 ccgggcttgc ggtcgtcgcc gcgttcggcg gcggttcggc tttcgctcag cacactcccg
    18061 cgtatcagcc gtcgcagcac tgcgacggga cgacaccggg cggcaacaac gtcccggtca
    18121 agaccaagat cgacttggcg aagcccgccc tatgcttcaa ccttcacggc ggtttcaagg
    18181 gggcggtctg ggtcgacttc gcgacgtcct cggtcgtgat cgacggagac tccacgaacc
    18241 acaccaccat ccgctgcgcc gacggatacg cgggcgtgcg gcttgcgggc agcaacggac
    18301 cgacgatcct gttcagccag ggcgacaact acgacccggg cgcctcgccg cgcgacggcg
    18361 ggaccaacgg ccatgctccg ggcagcccgc acgatccgtt cgacccgacc accgagcaca
    18421 acgagatcca caactgcttc atcggctagc gaaatagcct cgcgtccggg acggcgccgg
    18481 cgatagtctg cccggcatga atatccgccc catgaccgag gacgaccgcg aacccttcgt
    18541 cgcgctgatc cggcaggcgt tcaaccaggc tccgccgttc gtcgcgcgca tgcgcgagtt
    18601 gccgatcgac acctatcggg tcgccaccga atcgggccgg atcgtcgcgg ccgccgccgt
    18661 cctccccacc ggccacttct tcggcggcac gccggtggcg tgtgcgggcg tctccgccgt
    18721 cgtggtcgcg ccccacgcgc gcggcgggcg cacggccgaa attctcgtcg gcgagatgct
    18781 caaggagcgc cgcccggtga tgccgctgtc gtcgctgtac ccggcgacgg tgccgatcta
    18841 ccgccggctc ggctacgaat acggagcgat ccggtgcacg taccgcgtgc cgctgcatcg
    18901 tctgacgcgg ttcggcgacg ccctgccggc gacgccgtgg gacgacggcg atctggacga
    18961 ggttgcgacg gcacatcggg cgtgggcagc cgagcacaac ggtgtcatgg atcggacgcc
    19021 ctcatactgg gagcggctgt gcaaacagat aggggacgac cccgtctatc gcgtcctggt
    19081 tcgggagggc cgacaggtga ccgggtcgct gatctaccac caagaaaacg gctttcacat
    19141 ggatctggtg gcccatgatc tgttctggcg gacgccggcg gccgcccggt cgctgatcac
    19201 ctacgtcgcg cgacaccatt cgctcggccg cgacttcgtg tggaccggtc cttcgaccga
    19261 tccgatgctt ttccacagcg ccgaatacaa gatccgcgaa aaggagcgct ggcatctgat
    19321 gctccggctg ctcgacgttt ccgccgccgt ggtgtcgcgg ggctaccgga gcgggaccga
    19381 aaccgccgtc tccgtgggcg tgcaggatga gcttttcccc gagaattcgg ggccctggcg
    19441 catcgccgtc tcgggcggaa aggccaccgc cgagcccgca tccgacgccg agatcaccat
    19501 gggcgtgggc acgctggcaa gcatctatag cggcctgatc acgacgcac
//
LOCUS       DATLFO010000358         5624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18326, whole genome shotgun sequence.
ACCESSION   DATLFO010000358 DATLFO010000000
VERSION     DATLFO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18326"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1475
                     /locus_tag="VM841_14565"
     CDS             264..1475
                     /locus_tag="VM841_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91448.1"
                     /translation="MRTIIVGGGVAAASAAATLRAEGDDGPIVLIGDETHVPYDRPPL
                     SKSLLQGGDPDINARPDGFWASNDIELRLGVRVTAIDTDARIVDAGGERVPYHRLLIA
                     TGGRARALSVPGSDLDGIHLLRTVADARAIRADAERGGPACVIGMGFIGAEVAASLRR
                     RGNEVVAISPALPLERVLGPEMGRAIARLHAGHDVDLRLGPSIESFEGAGRVERIRTT
                     SGETIECDFVVAGVGIEVNAEPAAAAGIVTGNGIVVDAACRTSAPGVWAAGDVAEHAH
                     PLFGRIRVEHWQNAIEMGAAAARSMLGKADAYDRVHWFWSDQYDTVIQYAGHHREAAD
                     VVFRGDPESQRFLAFYRDASGAVRAVAGIDRGREVRRAMPLIASGTPVDGAILADEDV
                     DLKTIVSRRSL"
     gene            complement(1498..2100)
                     /locus_tag="VM841_14570"
     CDS             complement(1498..2100)
                     /locus_tag="VM841_14570"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVL91449.1"
                     /translation="MRHVIRTVMTLAALTAMLGGVLGQGSSPAASSMPGRQLAVTSVA
                     IDSRPLPGETPLARWAYAINTSPRISLNPDPEAPLGKAIEVSIADQRLTAWENGKIAH
                     RFKVSTARPGKKTPKGHFRIKLKAKRYWSRQFQVWMPDALWFHGDYFIHSLPYRSNPS
                     NRTGADMLGIPVSAGCVRLGVVESKVIYDWASVGTPVWVH"
     gene            2307..2735
                     /locus_tag="VM841_14575"
     CDS             2307..2735
                     /locus_tag="VM841_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL91450.1"
                     /translation="MRILVIDDEPDVLLLCRVNLAFEGHEVSEAADAPQGLEAAIAKN
                     PDLIVLDVMLPNGDGISVLRELQSRERTRHIPVILLTAKARGEDELVGFEAGATMYMT
                     KPFSPAALCEAVATVGGMTSDERRKARETSLRQLTVLQQQ"
     gene            2828..3823
                     /locus_tag="VM841_14580"
     CDS             2828..3823
                     /locus_tag="VM841_14580"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVL91451.1"
                     /translation="MRIEDLPTPALIADAGALEHNIAAMAAVLPGTRCRPHVKAHKCT
                     AIARRQADAGHRAFTCATIREMEGMAAAGLGEDLLLANEVLDARRLGALVDAGARVTV
                     AVDSGETISAAADGGVREVLIDVNVGLPRCGCAPEDAGRLADAARARGLFVRGVMGYE
                     GHAVGLPDRELRASITRESMERLAAAHAAVGGDVISAGGTGTYDLNTVATEIQAGSYV
                     LLDDAYDALGLPFRRGLGIVATVISVSNEHAVADAGLKAMGMDHGNPTIDRGHVVWFV
                     SDEHLSFAPAEPVRAGDRVTIRPAHIDPTIAYHERIHLVRDGEVLDTWPVDLRGW"
     gene            3826..5127
                     /locus_tag="VM841_14585"
     CDS             3826..5127
                     /locus_tag="VM841_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HVL91452.1"
                     /translation="MPGWGNWAGTASCRPARIVAPESDDELAWLLAEAFARGERVKIV
                     GSGHSFTDAACTNEIMIRSDRLTRVLDVDRAARRVTVQPGITIHALAERLAAEGLAFR
                     NLGDIGEQTIGGAIATATHGTGARSGTLSSQVSTIEMLAVDGRRVRCAPGSDGDLLAA
                     ARVSLGALGAMIAITLDVEEAYTLHAVEETMPLKAVLRSLESLADDNDHFELFWFPHT
                     ERALTKRNNRIDAPPRPRPRTRQWLDQELVANTLFGAVCRVGRARETLIPPMARLAGR
                     LLPRTEHSDRSDRIFTSPRRVRFAELEYAIPRPFAADALRAVRALIERRGYRISFPVE
                     LRFTAADDDAFLSPAAGRPTAYLAVHVFQGMPYEAIFRDVEALMAGFDGRPHWGKIHF
                     LAAEDLAGRYPGWDAFAAVRKRLDPGGLFRNDYLDRVLGVG"
     gene            5132..5269
                     /locus_tag="VM841_14590"
     CDS             5132..5269
                     /locus_tag="VM841_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91453.1"
                     /translation="MSGETAPACDTSTLCWMCGTDMKPEHAHYRCPSCGWRDSCCDGP
                     Y"
     gene            5275..>5624
                     /locus_tag="VM841_14595"
     CDS             5275..>5624
                     /locus_tag="VM841_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91454.1"
                     /translation="MPPADVRTERLRLIPCDAALLAAVMRDEPLPGLRRHFEWPGDDL
                     RGAIPIMLARLTERAETAGWGPWLIVLDGEVIGDAGTHGPPDETGEAEIGFAVVPALR
                     RRGIASEAVEALLG"
BASE COUNT          838 a         1911 c         2026 g          849 t
ORIGIN      
        1 ggatgacgcg ccggccccac atgaagcggt ccttcccgaa gccgtccccg atcggcgaga
       61 tcgacacgcc gccggggccg cccgaggggg atcatccatc cgtttccgag cgccatgccg
      121 gccggcacgt ccaggaagtc cgacgctgcc gccccgcgcc ggtccgatcg gtgccagtgc
      181 gagcgcaagc accaggacaa gtccataccc caacgcggaa gagagcgggg taccgtatgg
      241 cacgcgaacc gggaggcagg ggagtgcgaa cgatcatcgt gggcggcggc gtggccgccg
      301 ccagcgcggc cgcgacgctg cgcgccgaag gagacgacgg tccgatcgtg ctcatcggcg
      361 acgagacgca cgtgccctac gaccggccgc cgctttcgaa atcgctcctg caaggcggcg
      421 atcccgacat caacgcgcgc cccgacggct tctgggcgag caacgacatc gagcttcgcc
      481 tcggcgtgcg cgtgaccgcg atcgacacgg acgcccgcat cgtcgatgcg ggcggcgaac
      541 gggtgcccta ccaccggctg ctgatcgcga ccggcgggcg ggcgcgtgcg ttgagcgttc
      601 ccggctcgga tctggacggg atccatctgc ttcgcaccgt tgccgacgcg cgcgcgatcc
      661 gcgccgacgc cgagcggggc ggaccggcgt gtgtgatcgg tatgggtttc atcggcgccg
      721 aggtcgccgc atcgctccgc aggcgcggca acgaggttgt tgcgatttcg ccggcgctgc
      781 cgctggaacg ggtgctcgga cccgagatgg gccgcgcgat cgcccggttg cacgcaggcc
      841 atgacgtgga tctgcggttg ggcccgtcga tcgaatcctt cgaaggcgcc ggccgggtcg
      901 agcggatccg caccacctcg ggcgagacga tcgaatgcga cttcgtcgtg gccggcgtgg
      961 gcatcgaagt gaacgcagag ccggctgcgg ccgccgggat cgtcaccggc aacggcatcg
     1021 tcgtcgatgc ggcgtgccgc acgtcggcgc cgggtgtgtg ggcggccggc gacgtggccg
     1081 agcacgccca tccgctgttc ggccggatac gcgtggagca ctggcagaac gcgatcgaga
     1141 tgggggcggc ggcggcgcga tcgatgttgg gaaaggccga tgcctacgac cgcgtgcatt
     1201 ggttctggtc cgatcagtac gacaccgtga tccagtacgc gggccatcat cgcgaggcgg
     1261 ccgacgtggt gttccgcggc gatcccgaat cgcagcggtt cctggcgttc taccgcgacg
     1321 cgtcgggtgc ggtgcgcgcg gtcgccggca tcgatcgggg ccgtgaggtt cgccgcgcga
     1381 tgccgctgat cgcgtccggc acgccggtgg acggggccat ccttgccgac gaagacgtgg
     1441 atctgaagac gatcgtctcg cggcgttcgc tatgaccggg atcggcccgc cggcgggcta
     1501 gtgaacccaa acgggcgtgc ccacgctcgc ccaatcgtag atcaccttcg actcgacgac
     1561 gcccagccgg acgcagccgg ccgagaccgg gatgccgagc atgtccgcgc cggtacggtt
     1621 gctcgggttc gagcgatagg gaagcgagtg aatgaagtag tcgccgtgga accacagggc
     1681 atcgggcatc cagacctgga actggcgcga ccaataccgc ttcgccttca gcttgatccg
     1741 gaagtgcccc ttcggcgtct tcttgcccgg ccgggcggtc gagaccttga agcggtgcgc
     1801 gatctttccg ttctcccacg cggtcaggcg ctggtcggcg atcgaaacct cgatggcctt
     1861 gcccagcggc gcctcgggat cggggttgag agagattcgc ggtgaggtgt tgatcgcata
     1921 cgcccagcgt gcgagcggcg tctcgcccgg cagcggccgc gagtcgatgg cgacggaggt
     1981 gaccgcaagt tgccggccgg gcatcgagga tgcggccggc gaagagcctt ggcccagcac
     2041 gccgcccagc atcgccgtca gcgcggcgag ggtcatcacg gtccggatca catgtctcat
     2101 tcgtcctcta cgagggccaa ttgtggcacc ggacgggaga aatcgccaga ttcatggttg
     2161 ctccccgttg atacccccct ccggaatgcc catgggatgt gaccccggcc gcaccgacaa
     2221 atggccgtcg tggggggtgt tgtgccgggc cgggctccgg tagaaatgag gcaagagcgg
     2281 gcgctgagga aggaacgatt cacggtatgc gtattcttgt catcgatgat gagccggacg
     2341 tgctgcttct gtgcagggtc aacctcgcat tcgaagggca cgaggtgagc gaggcggccg
     2401 acgcgccgca aggtctcgag gccgcgattg ccaagaaccc ggatctcatc gtgctcgatg
     2461 tgatgctccc gaacggggac ggcatcagcg tcctgcgcga gctccagtcg cgtgagcgca
     2521 cgcggcacat cccggtgatc ctgctcaccg cgaaggcccg cggcgaagac gagctcgtcg
     2581 gcttcgaggc cggcgcgacg atgtacatga cgaagccgtt ctcgcccgcg gccttgtgcg
     2641 aggccgtcgc caccgtcggt gggatgacct ccgatgagcg acggaaggcg cgtgagacga
     2701 gcctgcgcca gctgacggtc ttgcagcagc agtagagacc ttcgcccggg cgcgccggat
     2761 ctccccccga ttcgccggcg ctccgggctg ccgcgaaacg gcccggccgt tgtaggctcg
     2821 cgcggcgatg cggatcgaag accttccgac gccggcgctg atcgccgacg ccggcgcgct
     2881 cgagcacaac attgccgcga tggccgccgt cctgccgggc acgcgctgcc ggccacacgt
     2941 caaggcgcac aagtgcaccg cgatcgcgcg acgccaggcc gatgcggggc atcgcgcgtt
     3001 cacgtgcgcg accatccgtg aaatggaggg catggccgca gccggcctgg gcgaggatct
     3061 gctgctcgcc aacgaggtgc tcgacgcgcg gcggctcggt gcgctcgtcg acgccggcgc
     3121 gcgggtgacg gtcgcggtcg actccggcga gaccatctcc gccgccgcgg acggcggtgt
     3181 gcgcgaggtt ctcatcgacg tcaacgtcgg gctgcctcgc tgcggatgcg cgccggagga
     3241 cgcgggccgc ctcgcggacg ccgcacgcgc gcgcgggctg tttgtgcgcg gcgtgatggg
     3301 ctacgaaggt catgccgtgg gcctgcccga tcgcgagctg cgcgcctcga tcacccgcga
     3361 gtcgatggag cggctcgcgg ccgcgcacgc agcggtcggt ggcgacgtga tcagcgcggg
     3421 cggcaccggc acctacgatc tcaacaccgt tgcgaccgag atccaggcgg gaagctacgt
     3481 gctgctcgac gacgcctacg acgcgctcgg cttgccgttc cggcgggggc tcggcatcgt
     3541 cgcgacggtg atctcggtgt cgaacgagca cgcggttgcc gacgccggcc tcaaggccat
     3601 gggcatggat cacggcaacc cgacgatcga ccgcggccat gtggtgtggt ttgtctcgga
     3661 cgagcacctc tcgttcgcgc cggccgagcc ggtgcgcgcc ggggaccgcg tgacgatccg
     3721 gcctgcccac atcgatccga ccatcgccta tcacgagcgc atccacctcg tgcgcgacgg
     3781 cgaagtgctc gacacctggc cggtcgacct tcgcggctgg tagcgatgcc gggatgggga
     3841 aactgggccg gcaccgcgtc gtgccgtccg gcacgcatcg tggcgcccga aagtgacgac
     3901 gagcttgcgt ggctgctcgc cgaggcgttc gcgcgcggcg agcgggtcaa gatcgtgggc
     3961 agcggccact ccttcactga tgcggcctgc acgaacgaaa tcatgatccg aagtgaccgg
     4021 ctgacgcgcg tgctcgacgt cgaccgcgcc gcacgccgcg tgacggtgca gccggggatc
     4081 accattcacg cgctcgccga acggcttgcg gcggaggggc tcgcgttccg caacctcggc
     4141 gacatcggcg agcagacgat cggcggcgcg atcgcgaccg caacgcacgg aacgggcgcg
     4201 cgttcgggaa cgctgtcgtc gcaggtgtcc acgatcgaga tgctcgccgt cgacggacgc
     4261 cgggtccgat gcgctcccgg atcggacggt gacctgctcg cggcggcacg ggtctcgctc
     4321 ggcgccctcg gagccatgat cgcgatcacg ctcgacgtcg aggaggccta cacgttgcac
     4381 gccgtcgagg agacgatgcc gctcaaggcg gtgctgcgct cgctggagtc cctcgcggac
     4441 gacaacgatc acttcgagct gttctggttc ccgcataccg agcgagcgct cacgaagcgc
     4501 aacaaccgga tcgacgcgcc gccgcggccg cggccgcgga cacggcagtg gctcgaccaa
     4561 gagctggtcg ccaacacgct tttcggcgcg gtgtgccggg tgggccgcgc acgcgagacg
     4621 ctcatccctc cgatggcgcg gctcgccggc cggctgctgc cgcggaccga gcactccgac
     4681 cgtagcgatc ggatcttcac cagccctcgc cgggtccgct tcgccgagtt ggagtacgcg
     4741 atcccgcgcc ccttcgccgc cgacgcgctg cgggcggtcc gggcgctgat cgaacggcgc
     4801 ggctaccgga tctcgtttcc ggtcgagctt cgctttaccg ctgcggacga cgacgcgttc
     4861 ctgtcgcccg ccgccggacg cccgaccgcc taccttgcgg tgcacgtttt ccaggggatg
     4921 ccctacgagg cgatcttccg cgacgtcgag gcgttgatgg ccggcttcga cggccggcct
     4981 cactggggca agatccattt cctggccgcg gaggatctcg ccggccgcta ccccggctgg
     5041 gacgcgttcg ccgccgtccg aaagcgcctg gatcccggcg gcctgttccg caatgactac
     5101 ctcgaccggg tgctcggggt agggtgatcg catgtctggt gaaaccgctc ccgcgtgcga
     5161 cacctcgacg ctgtgctgga tgtgcggaac cgacatgaag cccgagcacg ctcactatcg
     5221 ctgtccgtcg tgcggctggc gtgattcctg ctgcgacggc ccgtactagc tgtcgtgccg
     5281 ccggccgacg tccggaccga acgcctccgg ctgatcccgt gcgacgccgc gctgctcgcc
     5341 gcggtcatgc gggacgagcc cctgccgggg ctgcggcgcc atttcgaatg gcccggtgac
     5401 gatctgcgag gcgcgatccc gattatgctc gcgcggctca ccgagcgggc cgaaaccgcc
     5461 gggtggggac cgtggctgat cgtgctcgac ggcgaggtga tcggcgacgc gggaacgcac
     5521 ggcccgccgg acgagacggg cgaggcggag atcgggttcg cggtggtccc ggcgcttcgc
     5581 cgccggggca tcgcgagcga ggccgtcgaa gcgctgctcg gatg
//
LOCUS       DATLFO010000359         6987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18352, whole genome shotgun sequence.
ACCESSION   DATLFO010000359 DATLFO010000000
VERSION     DATLFO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18352"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..428
                     /locus_tag="VM841_14600"
     CDS             144..428
                     /locus_tag="VM841_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91455.1"
                     /translation="MFTDVASRMEAVLVAPVDLVRGLVSGIGGTTTEALMVAPLDVDG
                     RCAGIMIAGRSRFGDEDLERLWHLAEEAAPGFAAARLIERIRRRELRVAL"
     gene            465..941
                     /locus_tag="VM841_14605"
     CDS             465..941
                     /locus_tag="VM841_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91456.1"
                     /translation="MRVRTAAVVASRVLAIYVAFTSIIFAGELIKAIIERQPAGAIAE
                     PALPAAVALTLALVLWFAAPAVAARLAREPLGDDDPPARAREIALIAFTVAGLYIAAQ
                     ALPLAVHALARIALAEGTARDVTADLVSAAARTGIGVTLALLAPLLSRRLFPASPR"
     gene            1065..1718
                     /locus_tag="VM841_14610"
     CDS             1065..1718
                     /locus_tag="VM841_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91457.1"
                     /translation="MRANVASVLAVAIAALLTAGAPIAMRKASLLATEEAVGSRMIPG
                     AAAGIDVVITSRSQAGGQVRIPPPVAPAPSAEIPVRRGADDAPFGIVLPLGWSGGEVE
                     DFEDDAERSFRYQDEVGRYFVVNLDPMGSDFSADEVWEYGVAGDRFLVENERRCVPGE
                     AGCSVGDGERTIFAIWQEGGSAPVGAHTWYFRFGDSSSEIGGTRVFREILESIRVTV"
     gene            1820..2308
                     /locus_tag="VM841_14615"
     CDS             1820..2308
                     /locus_tag="VM841_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91458.1"
                     /translation="MSLRRIAATVFAVAALGTGLLVPSSSVAQGQCQRGETAAKFTQP
                     EGGQLSANTHLGGIAYTLPGSTTPLAVMLSTTGEVEVEIQHRCVQALRLIVYKDGEQA
                     PIHTNAWANLSCDEGVINDIVEIGLDGGSYRFELEGVGCDGSKLRSDGQGGLIVDPPL
                     GL"
     gene            2403..3437
                     /locus_tag="VM841_14620"
     CDS             2403..3437
                     /locus_tag="VM841_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVL91459.1"
                     /translation="MPRALAAALVVALMVPAAASSRTAASATLPPLTAAAAASYDVVG
                     ERFIRRVNADVRLAPASLAKMLVALIVAEQLTPDEQISISLRAANTKPDNIRWPAGAV
                     YTADELLHAMMMESSNGAAVAFADHIAGSVTTFSEIMDAKAASLGAHDTHIIEPSGLD
                     AEGQYSTANDMALIAAAVVRDPWLSKVVRTKTHVIPWKNGGKYTLHTLNGWIVRFDGA
                     IGVKNGYTTQAGNCLAAAATRFGRTVVTVVLNDRRWYADSVALMEDAFPRSVPGEVKP
                     LPPKTAAASADVRAAAAAVPAGRTSKLLAATMAMGAATLTVAARRRQVVRRRRARAAA
                     RRSRLPSLPG"
     gene            complement(3434..4717)
                     /locus_tag="VM841_14625"
     CDS             complement(3434..4717)
                     /locus_tag="VM841_14625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91460.1"
                     /translation="MGLQAPEVSRPRRSPEEELAARVLQQAAVARLGVRALSGLSFDA
                     LTAETVRLVAATMQADYVALFEALDVDDEVLLRAGHGWPPATVGTKMPAAEGSWVRAI
                     LDSSQPVIIEDLGGDERYHVRIMREVYGVRSSMGVAIVDTDRPYGVLSAHSTRTAAAF
                     SSDEVAFLQAIANLVGIARARTRSDEALLAALEREKDAVERLQSLNEMKNSFLEAVSH
                     ELRTPLASVLGFALTLQERKEGLSESEGAVMLERLVINAIKLDRLLRDLLDLDRLYRR
                     TIRPARRPTDLAELIRVAVAGVEAGGHPVHVPAGTFDAFVDAPKVERIVENLVVNATR
                     HTPGGSTIWVRLGRGPDGTVIAVEDDGPGVPDDLKGSIFDVFRRGGKSVPGTGVGLSL
                     VRRFAEMHGGGAWVEDRPGGGARFCVLLPDASPAG"
     gene            4849..5175
                     /locus_tag="VM841_14630"
     CDS             4849..5175
                     /locus_tag="VM841_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HVL91461.1"
                     /translation="MPTRSWSNKRERQYEHIKSGLLARGRKKDVAEEIAARTVNKERA
                     RSGEAKESSRLSREDISSGRRGGLRSHKGSAGRTYEQLYNEAKRKGIEGRSKMNKKEL
                     EKAVGR"
     gene            complement(5172..5735)
                     /locus_tag="VM841_14635"
     CDS             complement(5172..5735)
                     /locus_tag="VM841_14635"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HVL91462.1"
                     /translation="MADHDEDQRHDRSQSASRDAEYEALLQELRVLLPGVEILFAFLL
                     TIAFTEKFGAINGHLKWVYLVALGCSAFASVLLVTPSVYHRLRWREAHKEDVLRRSNA
                     IALSATGFLAVAATSGLYVASAVVFDDTVAAALAGVIGGATVLLWYGLPMYDRARGGS
                     RSRNEDAGSREERKPANTASPPHKKAS"
     gene            5887..>6987
                     /locus_tag="VM841_14640"
     CDS             5887..>6987
                     /locus_tag="VM841_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVL91463.1"
                     /translation="MRRLPVRGAALTFGVVVSLGLIPPPPAEATRSVVIHGGGFGHGR
                     GMSQWGARGMAASGSSWQAILAHYYRGASQARRHPGERIRVLVATAPAIVATSTQRFK
                     IQWRDGRLIGLNDPRRRFIRVRYAGGRYRVDKAATPAGPWRAVTHGMMPVVFVRGASP
                     IEIVATSGASSAYRQTVEVRHRDGRLDAINHLPLEEYLLGVVPREMPSSWPADALRAQ
                     AVAARSYAVAVMTRWKSRSYHICATTACQVYDGLAERRAQGAPSSPVERPATTAAVLS
                     TARIVLTWKGAPALTEYSSSTGGRTASGPRPYLRSVDDPSDRISPHHRWSVTVDASRI
                     ERSWRSIGRFRSLSVRARDGGGPWGGRVRTVRV"
BASE COUNT         1053 a         2482 c         2398 g         1054 t
ORIGIN      
        1 ggccgtgcgc ggctacgacg cggtagccgt ccgggtcggc cgcgtacagc gccgcggtct
       61 ccgaaccgga cggctaccgc gtcgtagccg cgcacggcct gtcgccggtc gagcagacga
      121 tcgtcgtccc gcacgagcag gccttgttca ccgacgtcgc atcgcggatg gaggcggtgc
      181 tggtggcccc ggtcgatctc gtgcgcggcc tggtctcggg catcggcggc accaccaccg
      241 aggcgctcat ggtcgccccg ctcgacgtcg acggccgctg cgccggcatc atgatcgctg
      301 gacgctcacg attcggcgac gaggatctgg agcggctctg gcatctggcc gaggaagccg
      361 cgcccggttt cgccgccgcg cgcctgatcg agcggatccg ccgaagggag ctacgcgtcg
      421 cgctctagcc ccgcaccggc gcgcgccggg tacgcttgcc cgtcatgcga gtccgcaccg
      481 ccgccgtcgt cgcctcccgt gtgctggcga tctacgtcgc cttcacctcg atcatcttcg
      541 ccggcgagct gatcaaggcg atcatcgagc gtcagccggc gggagcgatc gccgagccgg
      601 cgcttccggc cgcggtggcg ctcacgctcg cgctggtgct gtggttcgcg gcgccggccg
      661 tcgcggcgcg actcgcccgc gagccgctcg gcgacgacga cccgcccgcc cgcgctcgcg
      721 agatcgcgct tatcgctttt accgtcgcgg gtctgtacat cgcggcccag gcgcttccgc
      781 tcgcggtgca cgccctcgcg cgcatcgcgc tcgccgaagg aacggcccgc gacgtaacgg
      841 ccgatctcgt ctccgcagcg gcgcgcacgg gcatcggggt cacgctggct ctgctcgccc
      901 cgctcctgtc gcggcgcttg ttcccggcgt cgccgcgctg agcgttcgcg aaacgcggat
      961 caacccgagc aaaagggccc cgccggattg ggcatgttcg gctcacccgt gttcggggca
     1021 tcattaccct tcgtatgcgg ccgaacgggt ggagctttct acgagtgcga gcgaacgttg
     1081 cttccgtgct cgccgtcgcc attgccgcgc ttctgacggc aggggcgccg atcgcgatgc
     1141 gcaaggcctc gttgctggcc accgaggagg cagtcggatc gcggatgatc cctggtgcag
     1201 cggcgggcat cgacgtggtg atcacgagcc gttcacaggc cggcgggcag gtgcgcatcc
     1261 cgccgccggt cgctcccgct ccctcggcgg agatcccggt gaggcgcggc gccgacgacg
     1321 cacccttcgg gatcgtgctg ccgctgggct ggagcggggg cgaggtcgag gacttcgaag
     1381 acgatgccga gcgctcgttc cgctaccagg acgaggtcgg ccgctacttc gtggtgaacc
     1441 tcgacccgat gggcagcgac ttctcggccg acgaggtgtg ggagtacggc gtcgccggtg
     1501 accgcttcct ggtcgagaac gagcggcgct gcgtccccgg cgaagccgga tgctcggtgg
     1561 gcgacggcga gcgcaccatc ttcgccattt ggcaggaggg tggctctgca cccgtcggcg
     1621 cccacacctg gtatttccgc ttcggcgaca gctcgagcga gatcggcgga acccgcgtgt
     1681 tccgcgagat cctcgagagc atccgcgtga ccgtctaaca tttcgcccga aaacctcccc
     1741 aatgagtact ttggcacaga cggggcactg tctcgctgcc atgatggcct caccgcaaca
     1801 gtcaagaggg ggaggaacta tgtcgcttcg ccgcatcgct gccaccgtgt tcgccgtcgc
     1861 cgctctcggc accggtctgc tcgttccgtc gtcgtccgtg gcgcagggcc agtgccaacg
     1921 tggggaaacc gccgcgaagt tcacccagcc ggagggcggg cagctttccg ccaacacgca
     1981 tctgggcggg atcgcctaca cgctgccggg cagcaccacg ccgctcgcgg tgatgctgtc
     2041 gaccaccggc gaggtcgagg tcgagatcca gcaccgctgc gtgcaggctc tccgactgat
     2101 cgtctacaag gacggggagc aggcgcccat ccacaccaac gcctgggcca acctctcctg
     2161 cgatgagggg gtcatcaacg acatcgtcga gatcggcctg gacggaggaa gctaccgctt
     2221 cgagctcgaa ggtgtgggct gcgacggatc gaagcttcgg tccgacgggc agggcgggct
     2281 gatcgtcgac ccgccgctcg gcctgtagca ccatcgagga cggctccggc gcggcggccg
     2341 gagccgtcct cgcgtttcca ccttccgagg aatcggccgg acccgccgct agcatccggg
     2401 cgatgccccg cgctctcgcc gcggcgctcg tcgtcgcgct catggtcccg gccgccgcat
     2461 cctcgcggac tgccgcttcg gcgacccttc ctccgctgac ggctgcggcc gcggcttcct
     2521 acgacgtggt cggcgaacgg ttcattcgcc gcgtcaacgc cgacgtgcgc ctcgcgccgg
     2581 cgagcctggc caagatgctc gtcgccttga tcgtggccga gcagctcacg cccgacgagc
     2641 agatctcgat ctcgctgcgc gccgcgaaca ccaagccgga caacattcga tggccggcgg
     2701 gcgccgtcta caccgccgac gagctgctcc acgcgatgat gatggaatcg tccaacggcg
     2761 ccgccgtcgc cttcgccgac cacattgccg gctccgtcac tacgttttcg gagatcatgg
     2821 atgccaaggc cgcgtcgctc ggcgcgcacg acacccacat catcgagccc agcggcctcg
     2881 acgccgaagg ccagtattcg acggcgaacg acatggccct catcgcggcc gcggtcgtcc
     2941 gggatccgtg gctgtcgaag gtcgtgcgca ccaagacgca cgtgatcccg tggaagaacg
     3001 gcggcaagta caccctgcac acgctcaacg ggtggatcgt tcgcttcgac ggtgcgatcg
     3061 gcgtcaagaa cggctacacg acacaggcgg gcaactgcct tgcggcggcc gcgaccaggt
     3121 tcggccggac cgtggtcacg gtcgtgctca acgaccggcg ctggtatgcc gacagcgtgg
     3181 cgctgatgga ggatgccttc ccccggtcgg ttcccggcga ggtcaagccg cttccgccga
     3241 agaccgccgc cgcctcggcc gacgtgcgcg ccgcggccgc ggccgtcccg gccgggcgaa
     3301 cctcgaagct gctcgcagcc acgatggcga tgggcgccgc gacgctcacg gtcgccgcgc
     3361 gccgccgcca ggtcgtgcgc cgccggcgcg cgcgcgccgc ggcccggcgc tcgcgcctgc
     3421 cgtcgctgcc gggttagccg gccggcgacg cgtcgggcag caggacgcag aaccgcgcgc
     3481 cgccaccggg ccggtcctcg acccacgcgc cgcccccgtg catctcggcg aagcggcgga
     3541 ccagcgacag cccgacgccc gttcccggaa cgctctttcc gccccggcgg aacacgtcga
     3601 agatcgaccc cttgagatcg tcgggcacac cgggtccgtc gtcttcgacc gcgatcaccg
     3661 tcccatccgg cccccggccg agcctgaccc agatcgtcga gccccccggc gtgtgccgcg
     3721 tggcattgac gacgagattc tccacgatgc gctcgacctt gggcgcgtcc acgaacgcat
     3781 cgaacgtccc ggccgggacg tgcacggggt gaccgccggc ctccacgcct gcgaccgcca
     3841 cgcgaatcag ctcggcgagg tcggtcgggc gccgcgccgg acgaatcgtg cggcgataga
     3901 gccggtcaag atcgagcaga tcgcgcagca gccggtcgag cttgatcgcg ttgatgacga
     3961 gccgctcgag catgacggcg ccttcggact cgctcaaacc ctccttccgc tcctgcagtg
     4021 tgagcgcgaa cccgaggacc gaggccagcg gcgtgcgcag ctcgtgcgag acggcttcga
     4081 ggaacgagtt cttcatctcg ttgagcgact ggagccgttc gacggcgtcc ttctcgcgtt
     4141 ccagcgccgc gagaagcgct tcgtcgctcc gcgtccgcgc cctggcgatg ccgacgaggt
     4201 tggcaatcgc ctgcaggaac gcgacctcgt cgctcgagaa cgcggcggcg gtgcgagtcg
     4261 agtgggcgct caggacgccg taggggcggt cggtgtccac gatcgcgacg cccatcgagc
     4321 ttcgcacgcc gtagacctca cgcatgatcc gcacgtggta tcgctcgtcg ccgccgagat
     4381 cctcgatgat caccggctgg gaggaatcga ggatcgcgcg aacccagctg ccctcggccg
     4441 ccggcatctt cgttccgacg gtcgcgggcg gccatccatg gcccgcccga agcagcacct
     4501 cgtcgtccac gtcgagcgcc tcaaagagtg cgacatagtc ggcctgcatg gtcgcggcaa
     4561 ccagacgcac cgtctcggcg gtcagcgcgt cgaacgacag ccccgacagg gcgcgcacac
     4621 ccagccgcgc gactgcggcc tgctgcagca cacgcgcggc cagctcctcc tccggagagc
     4681 ggcgaggacg ggatacctcg ggcgcctgca accccacgcg acgatgcttc catcgggttg
     4741 tcctcgaagc aatagtcaga actggccagc cgttgcgcgt tccgcggacc ccttggttcg
     4801 gccagcaacc atccggggaa tgatcgtcca cggtcgaaag gaggaagcat gccgaccagg
     4861 tcgtggagca acaagcgtga acggcagtac gagcacatca agtccgggct gctcgcgcgc
     4921 ggacgaaaaa aggacgtggc cgaggagatc gcggcgcgga cggtgaacaa ggaacgcgcg
     4981 cgcagcggcg aagcgaagga gtccagccgg ctgtcgcgcg aggacatctc atcggggcgc
     5041 cgcggcgggc tgcggtcgca caagggctcg gccggccgga cctacgaaca gctctacaac
     5101 gaggcaaagc gcaagggcat cgaaggacgc tcgaagatga acaagaagga gctcgagaag
     5161 gcggtcggcc gctaagaggc cttcttgtgc ggcggcgacg ccgtgttcgc cggctttcgc
     5221 tcctcgcggg agccggcgtc ttcgtttcgt gagcgcgacc ccccacgggc ccggtcgtac
     5281 atcggcaagc cgtaccacag cagcacggtc gcgccgccga tgacgcccgc gagcgcggcg
     5341 gccaccgtgt cgtcgaacac gacggccgaa gcgacgtaca gacccgaggt cgccgcaacc
     5401 gccaggaacc cggtcgcgga cagcgcgatc gcgttggagc ggcgaagcac gtcttccttg
     5461 tgggcctcgc gccaacgcag gcgatggtag acgctgggcg tgacgagcag caccgacgcg
     5521 aatgccgagc atcccagcgc gacgaggtag acccacttca ggtgtccgtt gatggctccg
     5581 aacttttcgg tgaacgcgat tgtgagcagg aacgcgaaca gaatctccac cccgggcagc
     5641 agcacgcgca actcttgcag cagcgcttcg tactcggcgt cgcgggaggc agattggctt
     5701 cggtcgtgtc gctgatcttc gtcgtgatcg gccatgcgct tccttttccc gcaagcgacc
     5761 gccgccaatc caagcacctg cttcgccggg atcgcgaagt tcgtgcaagc gaaacttgga
     5821 aacctcaagc gaatttccag gaatctcctg gtgctggtga ggctgatggg tcgatagtgc
     5881 gccttcgtgc gccgccttcc cgttcgtggc gcggcactga ccttcggtgt cgtggtctcg
     5941 ctcggcctga ttcccccgcc gcccgccgag gccacccgct cggtcgtgat ccacggcggc
     6001 gggttcggcc atggccgcgg catgagccaa tggggtgcgc gcggcatggc cgcgtccgga
     6061 tcgagctggc aagcgatcct ggcgcactac taccggggcg cgtcgcaggc gcgtcggcat
     6121 cccggagagc ggatccgcgt gctggtcgcg accgcgcccg cgatcgtcgc caccagcacg
     6181 caacggttca agatccagtg gcgcgacgga cgcctgatcg gcctgaacga tccgcggcgg
     6241 cggttcattc gcgttcgcta cgccggcggc cggtatcgcg tggacaaggc cgcgacgccg
     6301 gcggggccgt ggcgagcggt cacgcacggc atgatgccgg tggtgttcgt gcgcggcgcg
     6361 tccccgatcg agatcgtcgc cacgtcgggc gcgtcgagcg cgtaccgtca gacggtcgag
     6421 gtgcggcacc gcgacgggcg cttggatgcg atcaaccacc ttccgctcga ggagtacctg
     6481 ctcggggtcg tgccgcgcga gatgccgtcg tcgtggccgg ccgacgcgct tcgcgcgcaa
     6541 gcggtcgccg cgcgaagcta tgccgtcgcg gtcatgacgc gatggaagag ccgcagctat
     6601 cacatctgcg ccaccacggc gtgccaggtc tacgacgggc ttgccgaacg ccgcgcgcag
     6661 ggcgcgccct cgtcccccgt ggagcgtcct gcgaccaccg ccgcagtgct ctcgaccgcg
     6721 cggatcgtgt tgacgtggaa gggcgcgccc gcgctgaccg agtacagctc gtccaccggg
     6781 ggcaggacgg cttcggggcc acggccctat ctgcgttccg tcgatgatcc ctccgaccga
     6841 atctcgccgc accaccggtg gtcggtcacc gtcgacgcct cgcgcatcga acggagctgg
     6901 cgttcgatcg gacgcttccg gtcgctgagc gtccgggcgc gcgacggcgg gggcccatgg
     6961 ggcgggcggg tgcgcacggt ccgcgtc
//
LOCUS       DATLFO010000360        23951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_78912, whole genome shotgun sequence.
ACCESSION   DATLFO010000360 DATLFO010000000
VERSION     DATLFO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23951
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78912"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VM841_14645"
     CDS             complement(<1..1386)
                     /locus_tag="VM841_14645"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL91464.1"
                     /translation="MSSRAAQPTTLARVREIFAQPGFGRFFSTRITSQVGDGIFQLAT
                     ADLLLFDNPGANPALTLAILSAVTLVPFSIVAPFVGVFIDRWDRRTILQVVPLVRAVL
                     AATIALGVGGETHGPLFYLLVLLVLSANRFFLATMSAVLPQFVPEDDLLVANSVSSTG
                     GSVSNVIGLGIGSALAAVVGGTATAGFAAIAFLGGALLARGLHVQRGLPKNVKPLRED
                     VRDVVIEMVDGVRRLRESAKATFALSAVSVNQFLIGAMTGVVASYFIKNLDLGVGAIT
                     GLLGVIAFGIGIGVATVPAIARIIGRERMIPLSFAVAAVTLFIVGITLSRVIVIASAW
                     LVGLAYAIIKIPVDTIVQEEMPDSYRGRAFAAYDMLFNVARVSGTIVAAILVGIETAP
                     AVSVVLLGAAYAGASLFSLHRTRRFAVTPMPQEPGPPAAEEPIVAPEPQPEPILEPEP
                     RPVVAAAAETHP"
     gene            complement(1540..2619)
                     /locus_tag="VM841_14650"
                     /pseudo
     CDS             complement(1540..2619)
                     /locus_tag="VM841_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
     gene            complement(2680..3351)
                     /locus_tag="VM841_14655"
     CDS             complement(2680..3351)
                     /locus_tag="VM841_14655"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVL91465.1"
                     /translation="MLELAVLGLLKEKSMHGYELKKRLDEQLGHFWRISYGSLYPSLK
                     RLAADGTIEISSPQGETSRRKNVYHITPKGEEAFTDMLESGGVNIADRDQFMLRFAFS
                     RYMQPETRKRLLEGRRGYLQERLLAMSASLKRMRDRMDEYSQELMQYGVNETEHDIRW
                     LTDMLAAEESGRGTAKKKKTATTRERAPRRPRSSNRTPKGLVDNPAAPRTGNLPSAQG
                     KPAGL"
     gene            3638..3997
                     /locus_tag="VM841_14660"
     CDS             3638..3997
                     /locus_tag="VM841_14660"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HVL91466.1"
                     /translation="MAAIDYRLAKRAVLRAVGVGMLSRMDVCDAHPDLLRAARHIGED
                     AEDACPICGENELRLVLYAYGRDLKGGNGYARRAEDLRELSARPNEVVCYIVEVCMEC
                     NWNHLVRSFSTGRRAAV"
     gene            4050..4736
                     /locus_tag="VM841_14665"
     CDS             4050..4736
                     /locus_tag="VM841_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91467.1"
                     /translation="MSLHSRLSVASGASAAGAATMLLAHRFEGTVPAVLIMVLAARAS
                     GPASALSAGEFARRLATAALRRQAAFASAWVLFAAAASVRAGSTVMADIAGAHGVAGL
                     ALARGPVLSIAGMWLAVLGVAIAIGGSFPARVGVRLAGPNGGMVLMPALARRLDLLGV
                     AVQILFVAALAAGAQIRSATDAIGWVAAAVALGAWTWFGRDAARLPIAPAVAFGLCAA
                     GLALVVGGRA"
     gene            4733..6289
                     /locus_tag="VM841_14670"
     CDS             4733..6289
                     /locus_tag="VM841_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91468.1"
                     /translation="MSSATLTQAALILPLGAAVFALLAGQSPVARGIARVLMAAAAAA
                     TATAGALAALGHDVLWQGFDPDAWTGLLAAGALAGAATAMSTRSVAVAAAAAVAGGTA
                     AGSILAPDVLYLGVLLVASTAAFAVTAYAAPAASPLRRARVLLPLGIADALALAAFVA
                     AGDQSLAIGPFSGTAGALLLAAAALRLGLIPSVVEDALRSGTGSLLLGPVRAQGALLA
                     TVALAGSSSRGLWLLALGALGAAIAAVRLMRSPDISALLVVPAAVAACGLGLGGHVAI
                     AGAAAALAVAFVAVVLLAGSGEPTLAAPVAAFAPVGGSLLGFGLTGGAVWRLAAARPE
                     FIPAAVALGAAAALSAVTAWQVTERARANPRPYGLRRPGSAWAVALTAAPAVAIAGFL
                     AFAPAFAYERAGSAVALAAGAGQALVSPPESIAEGLGIAFAALALAGLLIGGGPRGTI
                     PTGRAASPPRWMHGWAAALPAPRERSVEASRRASAKARPWLVLTAVAGIAALGLAVRL
                     VLVAGERGWL"
     gene            6280..8397
                     /locus_tag="VM841_14675"
     CDS             6280..8397
                     /locus_tag="VM841_14675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVL91469.1"
                     /translation="MVVKTKARRTGDDGAADERKPRYWLRRVVVLGGVGLAFGILLFG
                     IYYAFAKVPEPQDIATAQSSVVEDHKGRFVGRLHGSEDRAIVPLKQIPEITRNAVIAA
                     EDRGFYQHKGISPLGVVRAAVRNLMAREIKQGGSTITQQYVKNAFVGRERSFMRKFKE
                     AVVSVKLERKWSKDQILSAYLNTIYFGRGAYGIDAAARAYYGIPASRLNLAQSALLAG
                     IVRAPEKLEPAKNAEAAKKIRDRVLDGMAEMKMIEETARDEAKKAELATIARGTGISG
                     RGAYFLDAVRRELIERFGEDEVLRGGLRIRTTMDVEMQKTAEEAVAGVLDRATDPEAA
                     LVSVDPATGEIRAYVGGRDYAKQQFDLAAAKRQPGSSFKPLVLAAALENGSSLSSAYP
                     APATITLQTGGKPWTVSNYDKKSYGTLNLAAATADSVNTVYAQLIMDIGPEKVVEVAE
                     RLGIESKLDAVPSLALGTLEISPSELAGAYATMANDGKRVAAHMIARVTDRDGKELYR
                     FSDSAKKEMEPNVARNVTQAMVQVVKAGTGRSADIGRPMAGKTGTTENHRDAWFAGYT
                     PNLATVVWMGYPDGTRTMSNVRGKKVVGGSFPAQIWKAFMKKAHSGVKVKSFPGAEPI
                     APSGEPAPSPSGSQTAVPTGPPVTVVPEPTGTGSPSHEPPPSPAPTPTQSRSRPPSPS
                     PSPTPTSTNAQSSPSPGGGTAAA"
     gene            8394..9761
                     /locus_tag="VM841_14680"
     CDS             8394..9761
                     /locus_tag="VM841_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HVL91470.1"
                     /translation="MSEQDAAPAWSPEKERRVGRMAVVLMAVIGAIFAMIGGFVLKKQ
                     CEPPRAWDGYQYTHNCYTDILPLYGNRGGEFSYRPDCEVRDGEPSPDCRSVPFSEGGL
                     AYRDNRLEYPPLTGLAISAVNAPLQNGDAVAFFRVNALMLAMFGVIGAAAIAAAARNP
                     KRMVMYVMAPAMISYAFLNWDLLALGLMAVGLWAFTKERDVLAAGMLGLGAAAKIFPG
                     LIVPALMLARRREGKPAWPLMLYSIVWFAVPNLVVLLFAGVDGWWFPFEFQSERIPNF
                     ETVWYFILHHGSGLTDPDWGSFWFPAGGPFSKFAQYGSLAIFGIASLILLWRESVRGE
                     IRALTTAFGILILFLMTTKVFSPQFALWLLPFFVLVRVPWHAFAAFALADLFVLGAIW
                     GFLNSFIVGTDTDMFRNVLEVAVFVRYAALGYVLFWTRRAGEDLTGDPEPVPEGAGSA
                     AAAWA"
     gene            complement(9738..10679)
                     /locus_tag="VM841_14685"
     CDS             complement(9738..10679)
                     /locus_tag="VM841_14685"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL91471.1"
                     /translation="MPSSMHAHDHAHGHDHGGIEGVSPLGFTGERVIPGDPKWAWCFQ
                     AHLFGYHDLARRMTSRPERLLEIGCGEGYGASVLSRHAGHVVAGDVAADAVAHARDKY
                     HAPNISWVVFDAQKLPFADGAFGVTCSLQVIEHFPDAEAHLDEVVRVTEPGGLHYVTT
                     PNIDNMGEAEKDNPFHLRDFNAADLDAVLSARFDDVAVEGMFYVEDSPRYRRMVAAEA
                     KEERLRPKLRKVEDRLAKLPGALRVRLRPLARKAAGVSHWPLPEAERARNEIIAADFR
                     AGPDAEQSFCLIGVGRTPPRKGAPAKKGPDSGPGRSR"
     gene            complement(10688..11023)
                     /locus_tag="VM841_14690"
     CDS             complement(10688..11023)
                     /locus_tag="VM841_14690"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HVL91472.1"
                     /translation="MRISATEPAAEMVRRVRQEGRDNLVMVLGSGCCDSTAPFLYDNY
                     LPEPDSVPVGAVDGVEVLAPGWLAKLYGADDELILDVDQGVVNDSFSLESEFDARFTL
                     RVPERPEQR"
     gene            11252..11836
                     /locus_tag="VM841_14695"
     CDS             11252..11836
                     /locus_tag="VM841_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91473.1"
                     /translation="MRGVRRLMVVAAVAFGMFATLGATPAMAGLGTGQVTGTVSTDGI
                     GACPAGNPLCRKPQNNITFSGVLITGTFTSNDNSQVFAGSVKVGTSNSVTARSTTGGT
                     LADDAGVVFPFSVDDALSVTGNITNGAPPNGCAGTYQRAGSIVLVPLQCKFSINGRPV
                     NSAVTVVAQFSPTQGDGVTTPITQANFTGIYVAV"
     gene            11929..14634
                     /locus_tag="VM841_14700"
     CDS             11929..14634
                     /locus_tag="VM841_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91474.1"
                     /translation="MTGFGERSGGAGRRLRRAAVLGVAAAALLAVLPMRHGARATPPP
                     VREIGFMRFGFDARAAQPMAVDSVLHRGFYAGNDDHMHVYDLKALKKIGRFPITQIGS
                     TFAVDEANHRIFYRSSGPAPTCADSTLVVIDTRTFKVATPQVPCYGRQSVSFYVRGIS
                     YYAPANKLYVIGAGALESALLEISGVRSYRESTVIRQLDADTLALDWEIDLSDRCDTN
                     TNTPSAADPVVGRSARGVISYCYAGGQGRAVWIPLVSDRPVTGGDGNAVVRVSPAVGR
                     STTPIVDSGSGRLLILEDRPPFGPGVWVYDPEAQRFHGVVPSGVSLDHEGVRYDYWRG
                     FAGGTGRLYIQNRKGLVLADVRATPLPPGVSYPVLTNRTDGISPGPISIDARLGRLFY
                     PYPGRGGFVVVADEVPLARRAAADDPDAGTADIPEADGKTGRTFSSEATAFGVHVVNV
                     GGIPRLVNNADALCTSESGINDRDEFGRCPADRVVTPGNREFFVASSSLESGSDSGAV
                     AAAHVLRFASADAATDSDVRSIGRCGADRGGDLVAPVIAGACPNGEGQPLEPVAAGTQ
                     GTGGKGYPIPGAICTDFDGTKATDRGGDSPLGRAAVTCDSGSGAAEGSASAAAVEIPG
                     VLSVASTSSSVTTRATPKGVETITTAVASGVEIGGQIVIGRIATTAVTRAHGRTGTTI
                     ATFARDYSDVRGPGIRCAECDPAAVADAINRLLGAQMRARVPDAHVLASPRGYQGIVA
                     KEPGLIASDKAVNDDDTITVNGLDVIVYNDSSPANRGGSAGRSRVIVSLAGVEAESRY
                     GIYLLPDAGEIPGDAGSVVVFDPAPDIGGADPATEDPRPERPRPGMSAGPAGPAGPAE
                     AIVRSFAFVRNRPGEFGMLFVLWSLLAAPAYLALRRRAMSRVLDV"
     gene            14631..16097
                     /locus_tag="VM841_14705"
     CDS             14631..16097
                     /locus_tag="VM841_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL91475.1"
                     /translation="MSRTPSSGSGAWAAAGIVIAISLLSAFTARLPTVEPVVNASAPV
                     ADPGAAPAGGEAPVAAPAGSDGPVPVPAGSAPVTTVGGKVYDCAKGHNAGATDVGVTA
                     KEIRFAATVVKTGIAKDFLADAQFGMEAVRQKVNRQGGVCGRTIQITYQDDGWEPARG
                     QRIIEGWIQSKKFFGLAVNPSSEGVRGAIDSHVIRDAKFPVIGSDGQLIDQYQDPWVW
                     PVATSTASVMHIMARHAWQQGARRFGIVWDQNYRFGVEGHKAFLGSIKRLGGTVTSDV
                     GVQGGQQSYSNQVNDFIGSCGGTSFARCDFVAMLLEPATATRWVGDGGLGNGNQRPRV
                     GIGAPQPLFVDAFARDCGKYCDGMLVWTSFKPPLDPFDKEPAVATYLADLRRVNSKAD
                     ANNPHVQGAYIGMQLLVEALTRLGPAPTRLGIKAVLDTMTFETGLAPPMRFSKGRPQF
                     PNVKAQAFASLAQTGSFQGWRYANSGFIDDVDAGKDAA"
     gene            16099..16980
                     /locus_tag="VM841_14710"
     CDS             16099..16980
                     /locus_tag="VM841_14710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL91476.1"
                     /translation="MNDFFVNIILTMPLVGAYTMFALGIVFIYRGSKVLNLAHGAMAM
                     FPAYVAYSAAQRTSVFVALAVALVAGALLGLGVERIVVRRLRPSGPTAQTVGTVAVLG
                     LLIAIAARLWGTTPLLAPQIFPKQIYQVGNATLQLGSVGLFAVALAAAAAFIALFKYT
                     DLGLALRASANNPRAAGLVGIDPEKTTAVAWAFAGVLAALGGVLLAATTNLHPYTLSL
                     EVLPAFVAALLGGLDDVKGAVIGSIFVGMVIGIVPTLPVIGSTVGIQQAVLGLVAVVV
                     MGLRGQRFSASDVRSGL"
     gene            16977..18992
                     /locus_tag="VM841_14715"
     CDS             16977..18992
                     /locus_tag="VM841_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVL91477.1"
                     /translation="MRLAETDRRVYRMLARAAVVAALILAPWLPPLSSQPALVQAANL
                     AGEYALIAMSLVILTGWVGQISLGQGAFVGIGAFTTGLLAREAGIPFPINLPIVVAVT
                     AAVASLLGLVALRVRGLYLAVATLIFAYVADKFLFTSAWLVGEGGSSTIPNRTIGRPG
                     TVTFFDFRSKEVFYLVVLAAVAAAWYAAMNLRESKTGRAFFAVRGSETAAVSLGIDVT
                     RYKLLAFALSGALAGIAGTLMMTDLKTATPTGFDFTRSLFYLGIAVTGGIGSLGGAIA
                     ASGAFAFLNAIGQIWDTALTRAIADNLDIVSVGLLLLVLLAYPGGLGAAGAAVGKRLR
                     ERARSGRRESVSDEAPSRPARQRVPRRRGVERRALDFSHLESEPRDDELSVIGSAAAQ
                     QARRDWRTAQPAPLDLPEDRSQREPILEARNIVVRFGGLTAADDVTVEVRKSEIVGLI
                     GPNGAGKTTTFNAIAGLNEPASGTVRLYGQDATTLPVHVRAQMGVGRTFQLIQLFPQL
                     SVFENLLVATHLHNDTGVLRHLALTDAAIEAEREARRSVRRIIGMLGLEDVADRRVAG
                     LPFGVLRHVELARALVTRAPLLMLDEPASGLDNTETDEFARLLKFIRAELGVSILLIE
                     HDVRMVTSVCDYIYVINRGAPLADGTPSEIQRHPDVVAAYLGEAVHA"
     gene            18989..19516
                     /locus_tag="VM841_14720"
     CDS             18989..19516
                     /locus_tag="VM841_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91478.1"
                     /translation="MKRFALVALIALAACSGDPSVGDRASPRASSPPPAAASSPATAM
                     EGRTGGSPSPGTAPGAGVAPSAQASASTVAASLARPCVPAGQTQTMTVTGLKPNEVTG
                     YSTQYSDGSNELTNKSYTSGFGNGVAGPNGRYETTWVVPAAAPAGRAEVLVIWASGTE
                     PLRVSFMIPAGGQGC"
     gene            19518..20279
                     /locus_tag="VM841_14725"
     CDS             19518..20279
                     /locus_tag="VM841_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL91479.1"
                     /translation="MLEIRGIDVFYGPIQGLRGVSMTVGEGERVALVGANGAGKTTTL
                     RAISGMLAPAAGEILFEGTPIHGLKPNQVVARGIAHLPEGRELFTELSVEDNLRAGYW
                     TQRKNGSFAAQRDRVMDHFPILRERAAQAAGTLSGGEQQMLGVARALMSNPRLLIVDE
                     LSLGLAPKIVSQLFDILREVNVAGCAVLIVEQFVHMALANTDRAYVLAKGEVVLGGAS
                     ADLAADPKLVASYLGDDEPDDEPNDEPKRRGARKR"
     gene            20276..20848
                     /locus_tag="VM841_14730"
     CDS             20276..20848
                     /locus_tag="VM841_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91480.1"
                     /translation="MSPARSERWGSRKRRRRIVRIVVAVIVIAGVVAAIVVPRSRASR
                     RAERAAVQTYAQVVKDLSFEGGRILQQEVKPRITDLREGVVSPEQFRREADNWIRLYQ
                     NLRKSFADAGPPERLATAARLYDDAFVAYIEAFEAFREASRLDTPEARNEAITEAVPI
                     AERADQIFDRARGELGEQMRALGLTPPGDL"
     gene            complement(21040..22557)
                     /locus_tag="VM841_14735"
     CDS             complement(21040..22557)
                     /locus_tag="VM841_14735"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVL91481.1"
                     /translation="MSDVRSYQLYINGEFVDAADGATFETYNPSDGSLFATVAKGGRE
                     DMRRGIEAARTAFDSGVWSEMPQAERSERLLKLWKLLVDNLGDLSNIEAMDAGHTLRM
                     ANLFSIALGVEHLRALADLANKLGEWEPVPFNEMPAFAMGLVRREPYGVTAAITPWNF
                     PFILMMWKVAPALATGNSVVVKPATYTPCSTVEFAKLVAEADVLPPGVLNVVPGAGGA
                     AGEELASNPMIDKVAFTGSTEVGRRIMQLASSTVKKTTLELGGKSANIILDDADLDAA
                     VPGSLFGTFLHSGQICHSGTRLFVPASIHDEIVSRLVDAAESLTVGSAMSFETDMGPL
                     IHRTQYETVERYVQLGLEEGAKLATGGARVDVEGHEGGYYYKPTIFTGVENSMRIAQE
                     EIFGPVLSVIKYDSVEDAIKMANDSIYGLGGAVWSRDVPRAINVAKRIRTGTVWINDY
                     HLLSAYAPFGGYKQSGVGRELGAWGLREYQQTKYIHVDQTPGKDQKYWYQILGNL"
     gene            22700..23389
                     /locus_tag="VM841_14740"
     CDS             22700..23389
                     /locus_tag="VM841_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HVL91482.1"
                     /translation="MRRVRVAIAIALAVVPAAAWLAFAPPAPAAERSVRVVTGNRFDP
                     KDKFVVAGDTVVWTYDDANGAPHTVTRETTPERFDSSPSNCPRTNPLEPDDCLDSNNR
                     TYSHRFDRPGTYSYFCKVHGQSMSGTIHVAPRETAAPSPSPTRTPASPSPSPSGSPSG
                     SGSPDPSASPTGTSTASPSPEDTLTPTPSDTGTPAPSPGGGSGTKVALAVVALGSLGG
                     AGFLVYRRFLS"
     gene            23438..23827
                     /locus_tag="VM841_14745"
     CDS             23438..23827
                     /locus_tag="VM841_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVL91483.1"
                     /translation="MKWLTRSIIPSMAALAALIAFLPRTGPAVGLVTGDFAFTPQALV
                     VEQGEAITIRNDGDLTHSFTCPECGMRSINIQPGQAKIATFPTAGTFEFFCVYHRTEG
                     MEGRVTVEGDAPAAPEPAGSPTPDPSP"
BASE COUNT         3438 a         8470 c         8277 g         3766 t
ORIGIN      
        1 ggggtgcgtt tcggccgcgg ccgccacgac gggccggggt tcgggttcga gaatcggctc
       61 cggttggggt tcaggcgcga cgatcggttc ctcggcggcg ggcggtcccg gctcttgtgg
      121 catgggggtg accgcgaagc ggcgggtgcg atgaagcgag aacaacgacg cgcccgcgta
      181 ggccgctccg agcagcacga ccgataccgc cggcgcggtt tcgatcccga ccaggatcgc
      241 cgcgacgatc gtccccgaca cgcgcgcgac gttgaacagc atgtcgtagg ccgcgaacgc
      301 gcggccgcga tacgagtccg gcatctcttc ctggacgatg gtgtccacgg gaatcttgat
      361 gatcgcgtag gccaggccga ccagccatgc ggatgcgatc acgatcacgc gcgacaaggt
      421 gatgccgacg atgaacagcg tgacggcggc gacggcgaac gacagcggga tcatgcgttc
      481 gcggccgatg attcgcgcga tcgccggaac cgtcgccacg ccgatgccga tcccgaatgc
      541 gatcacgccc agcaaccccg tgatcgcacc cacgcccaga tcgaggttct tgatgaagta
      601 cgaggcgacg acgcccgtca tcgcgccgat caggaactgg ttcaccgaca ccgccgacag
      661 cgcgaaggtg gccttcgccg actcgcgcag gcgccggacg ccgtccacca tctcgatgac
      721 gacgtcgcgc acgtcctcgc gcagcggctt gacgttcttc ggcaggccgc gctgcacgtg
      781 cagcccgcgc gccagcagcg cgccgcccag gaacgcgatc gccgcaaagc ccgcggtcgc
      841 ggttccgccg accaccgccg caagcgccga accaatgccc agcccgatca cgttggacac
      901 cgatccgcct gtggacgaca ccgagttggc gaccagcaga tcatcctcgg gcacgaactg
      961 cggcagcacc gccgacatgg tggccaggaa gaatcggttc gccgacagca ccagcagcac
     1021 cagcagatag aacagcggac cgtgtgtctc gcccccgacc cccagcgcga tcgtcgcggc
     1081 cagcaccgcg cgaaccaaag gcacgacctg aaggatcgtt cgccggtccc accggtcgat
     1141 gaacacgccg acgaacggcg ccacgatgct gaacggaacc agcgtcaccg ccgacaggat
     1201 cgccagcgtc aacgccgggt tcgcacccgg attgtcgaac agcagcagat cggcggtggc
     1261 gagctgaaag atcccgtcgc cgacctgcga ggtgatgcgc gtggaaaaga agcggccgaa
     1321 gccgggctgc gcgaagatct cgcggacgcg cgcgagcgtc gtcggctgag ccgcccggct
     1381 gctcacgcgc gtcccgccgc ttccacggcg cgacgttcgt cagcgaacgt caaccacgtc
     1441 caaggtgatg tctcccgcgt gggaaccgtt gccgtttccg ttggcatgac cgttgccgtt
     1501 gccgtttccg ttggcgatga gcccggccgt ggcgggcacg ccggcgatga actcttcggt
     1561 catcgcgcgc gccacgtcgt cgggatactg gaccggcggc gacttcatga agaacgacgc
     1621 ggggccgacc aacggaccgc cgatgccgcg ctcgagcgcg accttgatgc agcgcacggc
     1681 gtcgatcacg acgccggccg agttgggcga gtcccacact tccagcttga ggtccaccgt
     1741 cagcggaacg tcgccgaact cgcggccttc caggcggatg tgcgcccact tgcggtcctt
     1801 gagccacggc acgtgatccg acggaccgat gtgcacgtcg cccttgccca gcgtccggcg
     1861 catgttcgag gtgacggcct gcgtcttgga gatcttcttg gactccagcc ggtcgcgctc
     1921 gagcatgttc atgaagtcca tgttgccgcc gaagttcaac tggtaggtgt ggtccaggtg
     1981 cacgccgcgg tcctcgaaca gcttggccag cacgcggtgg atgatcgtcg agccgacctg
     2041 gctcttgatg tcgtcgccga tgatcggcag ccgcgcctcg cggaaacgcg ccgcccactc
     2101 ggggttggag gcgatgaaga ccgggatgca gttgatgaag ccgacgccgg cctgcagcgc
     2161 cgcgtcggcg tagaacttgg tggcgtcctc gctgccgacc ggcaggtacg acaccagcac
     2221 gtcggcgccg gacgcgcgaa gcacctcggc gacgtccacc ggcgtctccg tggactcgtc
     2281 cacgatctcg cggtagtagc ggcccaggcc gtcgaacgtc gggccgcgca tgacctcgac
     2341 acccagcggg ggaacgtctg cgaactggat cgtgttgttc ggctcgctca cgatggcttc
     2401 ggagagatcg cggccgacct tcttcgcgtc cacgtcgaag gcggcgacga actcgacgtc
     2461 gctcacgtgc aacccgccga gcatcacgtg catcagtccg ggaacgtcat cggccggctt
     2521 cgcgtccgcg tagtagtgca cgccctgaac gagcgaggac gcgcagttgc cgacgcccgc
     2581 gatcgcgacc cgtaccttcc gcgttccgtt gttgttcatt ctctgttccc ctttcgagcc
     2641 ccgtcgcgta gcacggggac gtttgtgcgt cccggcgtcc tacagtccgg cgggctttcc
     2701 ttgagcgctc ggcagattcc cggttcgcgg cgcggccgga ttgtccacga ggcccttcgg
     2761 tgtcctgttg gatgaccgcg gccggcgggg cgcacgctcg cgcgtcgtcg ccgtcttctt
     2821 cttcttggcc gtgccccgtc cgctctcttc ggcggcgagc atgtcggtca gccagcgaat
     2881 gtcgtgctcg gtctcgttca ctccgtactg catgagctct tgcgagtact cgtccatgcg
     2941 gtcgcgcatg cgcttgagcg aagcggacat cgccaggaga cgctcctgga gataaccgcg
     3001 tcgcccttca agcaagcgct tgcgggtctc aggctgcatg tagcgggaga acgcgaaccg
     3061 gagcatgaac tggtcccggt cggcgatgtt cacgccgccc gactccagca tgtcggtgaa
     3121 ggcttcctcg cccttggggg tgatgtggta gacgttcttg cggcgcgagg tctcgccctg
     3181 aggggaagag atttcgatcg tgccgtcggc cgcgagccgc ttcagcgagg gatacagcga
     3241 gccgtaggag attcgccaga agtggccgag ctgctcgtcc agccgcttct tgagctcgta
     3301 gccgtgcatg gacttctctt tcagaagccc cagaacggcc agttccagca tcgcggtttt
     3361 cctctcgctt tccggtcgct ttccggtcgc ttcctatcgg ctcgatatat cgaaccgata
     3421 tatcgatgcg atgcattgtg cccacggctc cggggggtgt caaacacctc cggggcagca
     3481 ctggcagatg ttgccaggcg attgtcgcgc gcttgtttcg ggcatctagc aggcaatcaa
     3541 cgagacacca gaccagtcca gttgccgata tgtccgaaat gttgcgaaag ccgacgctgc
     3601 ccggagaccg gtccccggcg gtacaatcgc tgaccttatg gcggcgatcg actaccggct
     3661 ggcaaagcgg gccgtgctcc gcgcggttgg ggtcggcatg ctctcgcgaa tggacgtctg
     3721 cgacgcccac ccggatctgc ttcgcgccgc ccggcacatc ggcgaggacg ccgaggacgc
     3781 ctgtcccatc tgcggtgaga acgagctgcg gctggtcctg tacgcctacg gccgcgacct
     3841 caaagggggc aacgggtacg cccggcgcgc ggaggacctc cgcgagctgt ccgcccggcc
     3901 gaacgaggtc gtctgctaca tcgtcgaggt gtgcatggag tgcaactgga accacctggt
     3961 ccgctcgttc tcgacgggcc gccgcgccgc cgtctgacgc gcttcgcccc tgcacagcgg
     4021 cgagcccggt agcgcgtagc ctgacgcgca tgagcctcca ctcccgactt tccgttgcat
     4081 cgggcgcgtc cgcagcgggc gccgccacga tgctcctcgc gcatcgcttc gaaggcacgg
     4141 tgccggccgt gctgatcatg gttcttgcgg cccgggcgtc cggccctgcc tccgccctgt
     4201 cggcgggcga gttcgcccgg cgcctcgcga cggccgccct gcggcgacag gctgcgttcg
     4261 cgtcggcgtg ggtgctgttc gcggccgcgg cctcggttcg cgccggctcc accgtgatgg
     4321 ccgacatcgc cggcgcgcac ggtgtcgccg gcctcgccct cgcgcgcggc ccggtgctct
     4381 cgatcgccgg gatgtggctc gccgttctcg gggtcgcgat cgcgatcggc gggtcgtttc
     4441 ccgctcgggt cggagtccgg ctcgcgggcc cgaacggcgg gatggtcctg atgcccgcgc
     4501 tggcccgccg cctcgacctg ctcggggtcg ccgttcagat cctgttcgtg gcggcgctcg
     4561 ccgcgggcgc gcagatccga agcgccaccg acgcgatcgg atgggtcgca gccgccgtcg
     4621 cgctgggggc ctggacctgg ttcggacggg acgcggctcg ccttcccatc gctccggccg
     4681 tggcgttcgg gctgtgcgcc gccggcctcg cgctcgtcgt cggaggtcgc gcatgagctc
     4741 cgcgacgctg acgcaagccg ccttgatcct gcctctgggc gccgccgtct tcgcgctgct
     4801 ggccgggcag agccctgtgg ctcgcggaat tgcccgcgtg ctgatggccg cggccgccgc
     4861 ggccaccgcc accgccggcg cgctggccgc tctcggtcac gacgtgctct ggcagggctt
     4921 cgatcccgac gcctggaccg ggctgctggc cgccggcgcg cttgccggag ccgcaaccgc
     4981 gatgagcaca cgaagcgtgg ccgtcgctgc ggccgccgcc gtcgcgggcg gaaccgccgc
     5041 gggctcgatc cttgctcccg acgttctcta tctcggcgtg ctgctcgtgg cctcgacggc
     5101 cgcgttcgcc gtgacggcgt atgcggcccc ggcggcctcg ccgttgcggc gggcccgcgt
     5161 gctgctgccg ctgggcatcg cggacgcgct cgccctcgcc gcgttcgttg ccgccggcga
     5221 tcagtcactc gcgatcggac ccttctccgg cacggccggc gccctgctgc tcgcggcggc
     5281 cgcgctcagg ctcgggctga tcccttcggt cgtcgaggat gcgctgcgct cgggaaccgg
     5341 gtcgctgctg ctcggacccg tccgggccca aggcgcgttg ctcgctaccg tcgcgctcgc
     5401 cggctcgtcg tcgcgggggc tgtggctgct cgcgctcggc gcgctcggcg cggccattgc
     5461 cgcggttcgc ctcatgcgct cccccgacat ctcggcgttg ctcgtcgtgc ccgccgccgt
     5521 cgccgcgtgc gggctcgggc tcggcggcca tgtcgcgatc gccggtgccg cggccgcgct
     5581 ggccgtggcc ttcgtcgccg tcgttttgct cgccggctcg ggcgagccca cgctcgccgc
     5641 gcccgtcgcc gcgtttgcgc ccgtcggcgg ttcgttgctc ggcttcggcc tgaccggcgg
     5701 cgccgtgtgg cgcctggccg cggcacgccc ggagttcatt cccgccgcgg tggcgctcgg
     5761 cgccgctgcg gccctgtcgg ccgtcaccgc ctggcaggtc accgagcggg cccgcgccaa
     5821 cccccggccc tacgggctgc ggcgtccggg cagcgcatgg gccgtcgcgc tcacggccgc
     5881 gccggccgtg gcgatcgccg gcttcctcgc cttcgccccc gcgttcgcct acgagcgggc
     5941 gggcagcgcg gtcgcgctcg cggccggcgc gggtcaggcg ctggtcagcc cgcccgagtc
     6001 gatcgccgag gggctcggca ttgcgttcgc ggcgctcgcg ctcgcggggc tgctcatcgg
     6061 aggcggcccc cgcggcacca tacccaccgg ccgtgccgcc tcgccgccgc ggtggatgca
     6121 cggctgggcc gccgcgctgc ccgcaccgcg cgaacggtcc gtcgaggcct cgcgccgtgc
     6181 gtcggcgaag gcgcgaccgt ggctcgtcct caccgccgtc gccggcatcg cggcgctcgg
     6241 gttggcggtg cggttggttc tggtcgcagg agagcgggga tggttgtaaa gaccaaggcc
     6301 cgccggacgg gcgacgacgg tgccgcggac gagcgcaagc cgcgatactg gcttcgccgc
     6361 gtcgtggtgc tcggaggggt cgggctcgcc ttcggcatcc tgctgttcgg catctactac
     6421 gccttcgcca aggtgcccga gcctcaagac atcgcgaccg cgcagtcgag cgtcgtcgag
     6481 gatcacaagg gccgcttcgt cggccggctt cacggcagcg aggatcgcgc gatcgttccc
     6541 ctcaagcaga tccccgagat cacgcgcaac gccgtcatcg ccgccgagga tcgcggcttc
     6601 tatcagcaca aggggatctc gccgctgggc gtggtgcgtg cggccgtccg caacctgatg
     6661 gcccgcgaga tcaagcaggg cggctcgacg atcacccagc agtacgtgaa gaacgccttc
     6721 gtggggcggg agcgatcgtt catgcgcaag ttcaaggaag ccgtggtctc ggtcaagctc
     6781 gagcggaagt ggagcaagga tcagatcctg agcgcctacc tgaacacgat ctacttcggc
     6841 cgcggtgcct acggcatcga cgcggccgcg cgcgcctact acggcatccc ggcgtcgcgg
     6901 ctgaacctgg cgcagtcggc gctgctggcc gggatcgtcc gtgcgcccga gaagctcgag
     6961 cccgccaaga acgccgaagc cgcgaagaag atccgcgacc gggtgctcga cggtatggcc
     7021 gagatgaaga tgatcgagga gaccgcgcgg gacgaggcca agaaagccga gctcgccacg
     7081 atcgcccgcg ggaccggcat cagcggccgc ggcgcgtact tcctggacgc ggtccggcgc
     7141 gagctgattg agcgcttcgg cgaggacgag gtgctgcgcg gcgggctgcg gatccgcacc
     7201 accatggacg tcgagatgca aaagaccgcc gaggaggccg tcgcgggagt gctcgaccgt
     7261 gccaccgacc cggaggccgc gttggtgtcg gtcgatccgg ccacgggtga gatccgcgcg
     7321 tacgtcggcg ggcgcgacta cgccaagcag cagttcgacc tcgccgccgc caagcgccag
     7381 cccggctcgt cgttcaagcc gctggtgctg gcagccgcgc tcgagaacgg ctcgtcgctg
     7441 tcgagcgcct atccggctcc ggccaccatc acgctgcaga ccggcggcaa gccgtggacg
     7501 gtgtcgaact acgacaagaa gagttacggc acgctcaacc tcgccgccgc cacggcggac
     7561 agcgtcaaca ccgtctacgc gcagctcatc atggatatcg gtcccgaaaa ggtcgtcgag
     7621 gtcgccgagc ggctcggcat cgagtcgaag ctcgacgcgg tgccctcgct cgcgctcggg
     7681 acgctcgaga tctccccgtc cgagctggcc ggcgcctacg cgacgatggc caacgacggc
     7741 aagcgcgtcg ccgcacacat gatcgcgcgc gtgaccgacc gtgacggcaa ggagctctac
     7801 cgattctcgg actcggccaa gaaagagatg gagcccaacg tggcgcgcaa cgtcacgcag
     7861 gcgatggtgc aggtggtcaa ggccggaacc ggccgcagcg ccgacatcgg gcggccgatg
     7921 gccggcaaga ccggcacgac cgaaaatcac cgcgacgcct ggttcgccgg ctacacgccc
     7981 aatcttgcca ccgtcgtctg gatggggtat cccgacggga cgcgtaccat gtccaacgtg
     8041 cgaggaaaga aggtcgtggg cggaagcttc ccggcgcaga tctggaaggc cttcatgaag
     8101 aaggcccaca gcggcgtcaa ggtcaagtcg ttccccggcg ccgagccgat cgctcccagc
     8161 ggcgagccgg cgccctcgcc cagcggatcg cagaccgccg tgccgaccgg tccgccggtc
     8221 accgtcgtgc cggagccgac cggcaccggc tctccgagcc acgagccgcc gccgtcgccg
     8281 gccccgacgc ccacgcagtc ccggtcccgt cctccctcgc ccagcccttc cccgacgccg
     8341 acgtcgacga acgcgcagtc gtcgccgtcg cccggtggcg ggacggcggc ggcgtgagcg
     8401 aacaggacgc cgctccggcc tggtcgccgg aaaaggaacg ccgggtcggc cgcatggccg
     8461 tcgtgctgat ggcggtgatc ggcgcgatct tcgcgatgat cggcggcttc gtgctcaaga
     8521 agcagtgtga gccgccccgc gcctgggacg gctatcagta cacgcacaac tgctacaccg
     8581 acatcctgcc gctgtacggc aaccgcggcg gcgagttctc gtaccggccc gactgcgagg
     8641 tgcgcgacgg cgagccgtcc ccggactgcc gctcggtccc gttcagcgag ggcggtctcg
     8701 cctatcgcga caatcggctc gagtatccgc cgctgaccgg cctggcgatc tcggcggtga
     8761 acgcgccgct gcagaacggc gacgccgtcg cgttcttccg ggtgaacgcg ttgatgctcg
     8821 cgatgttcgg ggtgatcggc gcagccgcga tcgcggcggc ggcgcgcaat cccaagcgca
     8881 tggtcatgta cgtgatggcg ccggcgatga tctcgtatgc gttcctgaac tgggacctgc
     8941 tggctctggg attgatggcg gtcgggctgt gggccttcac gaaagagcgc gacgtgctcg
     9001 ccgccggaat gctggggctg ggggcggccg ccaagatctt tcccggcctg atcgtgccgg
     9061 cgctgatgct cgcgcggcgc cgcgagggca agcccgcgtg gccgctgatg ctgtactcga
     9121 tcgtgtggtt cgcggtgccc aacttggtcg tgctgctgtt cgccggggtg gacggctggt
     9181 ggtttccctt cgagttccag tccgagcgga tccccaactt cgagaccgtc tggtacttca
     9241 tcctccacca cggcagcggg ctgaccgatc cggattgggg ctcgttctgg ttccccgccg
     9301 gaggcccctt cagcaagttc gcccagtacg ggtcgctcgc gatcttcggc atcgcgtcgc
     9361 tcattctgct gtggcgcgag tccgtgcgcg gagagatccg cgcgctcacc acggccttcg
     9421 gcatcctgat cttgttcctg atgaccacga aggtgttctc gccccagttc gcgctgtggc
     9481 tgctgccgtt cttcgtcctg gtgcgcgtgc cctggcacgc cttcgccgcc ttcgcgcttg
     9541 ccgatctgtt cgtgctcggg gcgatctggg gcttcctgaa ctcgttcatc gtcggcaccg
     9601 acaccgacat gttccggaac gtgctcgagg tcgccgtctt cgtgcggtac gcggcgctcg
     9661 gctacgtgct gttctggacc cggcgcgcgg gcgaggacct caccggcgat cccgagccgg
     9721 tacccgaagg cgccggctca gcggctgcgg cctgggcctg agtccggccc cttcttcgcc
     9781 ggggccccct tgcgcggcgg ggttcgtccc acgccgatca ggcagaagga ctgttccgcg
     9841 tccggcccgg cgcgaaagtc ggcggcgatg atctcgttgc gggcgcgctc ggcctccggc
     9901 agcggccagt gcgatacgcc ggctgccttg cgcgcgagcg gacgaagccg cacgcgcagc
     9961 gcgccgggaa gcttcgcgag ccggtcctcc acctttcgca gcttcggccg cagccgttcc
    10021 tccttcgcct ccgccgccac catgcggcgg tagcgcgggg agtcctcgac gtagaacatt
    10081 ccctccaccg cgacgtcgtc gaagcgggcc gacagcaccg cgtccagatc cgcggcgttg
    10141 aagtccctga gatggaacgg gttgtccttc tcggcctcgc ccatgttgtc gatgttcggg
    10201 gtggtcacgt agtgcagccc gcccggctcg gtgacgcgca cgacctcgtc gagatgggcc
    10261 tccgcgtccg ggaagtgctc gatcacctgc aacgagcagg tcaccccgaa ggccccgtcg
    10321 gcaaaaggaa gcttctgcgc gtcgaagacc acccacgaga tgttcggcgc gtgatacttg
    10381 tcgcgcgcgt gggcgaccgc gtcggcggcg acgtcgccgg cgaccacgtg accggcgtgc
    10441 cgggacagca cggaggcgcc gtagccttcg ccgcagccga tctcgagcag ccgctcgggc
    10501 cgggacgtca tgcgacgagc gagatcgtga tagccgaaca ggtgcgcctg aaagcaccag
    10561 gcccacttcg ggtcgcccgg gatgacgcgc tcgccggtga agcccagcgg cgagacgccc
    10621 tcgatgcccc cgtggtcatg accgtgagcg tggtcgtgcg catgcatcga cgagggcacg
    10681 ggcggcctca gcgctgctcg gggcgctcgg gcacgcgcag cgtgaagcgc gcgtcgaact
    10741 cgctttcgag cgagaacgaa tcgttcacca caccttggtc cacgtccagg atcagctcgt
    10801 cgtcggcgcc gtagagcttg gcgagccagc ccggcgcgag cacctcgacg ccgtccacgg
    10861 cgccgaccgg cacgctgtcg ggctcgggca ggtagttgtc gtagaggaac ggcgcggtcg
    10921 aatcgcagca gccggacccg agcaccatca cgaggttgtc gcggccctct tgccgcacgc
    10981 gacgcaccat ttcggcggcc ggctccgttg cgctgattcg catgtcccga ttctacccgg
    11041 ccgtcatttg cgaccggccg gttgggaaaa cctgtacgaa atgcacgcga aacagaagga
    11101 gtgaacagcg agcgagtcga actgtgccag caggccggag ggccgggtga gggatcggcc
    11161 gcactcaggg agcggccgcg agcacggggc gcgaccgacg aagcgccttc gccaggcgag
    11221 agatttgtcc gattctcggg gaggagattc gatgcgggga gtcagaaggc tcatggtcgt
    11281 cgcggcggtc gcgttcggca tgttcgcgac gctgggggcg acgccggcga tggccggtct
    11341 ggggaccggt caggtgacgg gcacggtttc gaccgacggt atcggggcgt gcccggcggg
    11401 caacccgctg tgccgcaagc cgcagaacaa catcacgttc tccggggtgc tcatcaccgg
    11461 caccttcacc tccaacgaca acagccaggt gttcgcaggc agcgtcaagg tcggaacgtc
    11521 gaactcggtg acggcccgga gcacgaccgg cgggacgctg gccgacgacg cgggcgtggt
    11581 gttcccgttc agcgtggacg acgcgctcag cgtgaccggg aacatcacca acggcgcgcc
    11641 gccgaacgga tgcgcgggca cctatcagcg tgcggggtcc atcgtgctcg tgccgctcca
    11701 gtgcaagttc tcgatcaacg gccgtccggt gaactctgcg gtgacggtcg tggcccagtt
    11761 ctcgccgacg cagggcgacg gggtcacgac gccgatcacg caggcgaact tcaccggcat
    11821 ctacgtcgcg gtctagcacg cgcgatagcg ttcgggggcc gggccatcaa gcgcccggcc
    11881 cctcgttcct tcctccgcga ggaggcgggt gaaggtttcg gaacagcgat gacgggattc
    11941 ggcgagagga gcggcggggc gggccgccgg ttgcggcggg ccgccgtgct cggcgttgcc
    12001 gccgcggcgc tgctcgcggt gctgccgatg cgtcacgggg ctcgtgcgac gccgccgcct
    12061 gtgcgcgaga tcggcttcat gcgcttcggc ttcgacgccc gggctgcgca gccgatggcc
    12121 gtggactcgg tcctgcatcg cggcttctac gcgggcaacg acgaccacat gcacgtctac
    12181 gatctgaagg cgctcaaaaa gatcggccgc ttccccatca cccagatcgg ctcgaccttc
    12241 gcggtggacg aggcgaacca tcggatcttc tatcgctcct ccggtcccgc gccgacctgc
    12301 gcggactcca cgctggtggt gatcgacacg cgcaccttca aggtcgccac gccacaggtc
    12361 ccctgctacg gccggcagag cgtgagcttc tacgtgcgag ggatctccta ctacgcgccg
    12421 gcgaacaagc tctacgtgat cggcgcgggc gcgctcgagt cggcactgct cgagatcagc
    12481 ggtgtgcgca gctatcgcga aagcacggtg atccgccagc tcgacgccga cacgctcgcg
    12541 ctggactggg agatcgacct gtcggaccgg tgcgacacga acaccaacac cccgagcgcg
    12601 gccgatccgg tggtaggccg aagcgcgcgc ggcgtgatct cgtactgcta cgcgggcggg
    12661 cagggccgcg ccgtgtggat cccgctggtg agcgaccgtc cggtcaccgg cggcgacggg
    12721 aacgccgtcg tgcgcgtcag cccggcggtc ggccgctcga ccacgccgat cgtggactcc
    12781 ggcagcggcc gtctgctgat cctcgaggac cgtccaccgt tcgggccggg cgtgtgggtc
    12841 tacgatcccg aggcgcagcg cttccacggc gtcgtgccgt ccggtgtctc gctcgaccac
    12901 gagggcgttc gctacgacta ctggcgcgga ttcgcaggcg gcaccggccg gctctacatc
    12961 cagaaccgca aagggctggt cctggccgac gtgcgggcga caccgctgcc gccgggcgtg
    13021 agctacccgg tgctgaccaa ccggaccgac ggtatctctc cgggaccgat ctcgatcgac
    13081 gcgcgtctgg gccgcctgtt ctatccgtat cccggccggg gcggcttcgt cgtggtcgcc
    13141 gacgaggttc cgctcgcccg ccgcgccgcc gcggacgacc cggacgccgg caccgccgac
    13201 atccccgaag ccgacggaaa gaccggccgc accttctcga gcgaagcgac cgccttcggc
    13261 gtgcacgtcg tcaacgtcgg cggcatccct cggctcgtca acaacgcgga cgcgctgtgc
    13321 acgtcggagt cgggcatcaa tgatcgcgac gagttcggcc ggtgtcccgc ggaccgcgtg
    13381 gtcacgcccg gcaaccgcga gttcttcgtc gcgtcctcgt cgctggagtc cggatcggac
    13441 tcgggtgccg tcgccgcggc gcacgtgctg cgcttcgcga gcgccgacgc ggccaccgac
    13501 tccgacgtcc gctcgatcgg ccgctgcggc gccgaccgcg gcggcgacct cgtcgcgccc
    13561 gtgatcgcgg gtgcgtgccc caacggggaa gggcagccgc tcgagccggt cgccgccgga
    13621 actcagggga ccggcggcaa ggggtatccg attcccggcg cgatctgtac cgacttcgac
    13681 ggaacgaagg cgaccgaccg gggaggcgac tcgccgctcg gacgcgcggc tgtgacctgc
    13741 gacagcggct ccggagcggc ggagggcagc gcctcggccg cggcggtcga gatcccgggc
    13801 gtgctgtcgg tcgcatcgac gtcgtcgagc gtgacgaccc gcgcaacgcc caagggagtc
    13861 gagacgatca cgaccgcggt cgcctccggc gtcgagatcg gcggtcagat cgtcatcggc
    13921 cggatcgcga ccacggccgt cactcgcgca cacggccgta ccggcaccac gatcgcgacg
    13981 ttcgcgcgcg actacagcga cgtccgcggc cccggaatcc gctgcgccga atgcgatccg
    14041 gcggcggtcg cggacgcgat caaccgtctc ctcggcgcgc agatgcgcgc ccgcgtgccg
    14101 gatgcgcacg tgctcgcgag cccccgcggc taccaaggca tcgtcgccaa ggagcccggc
    14161 ctgatcgcct ccgacaaggc ggtcaacgac gacgacacca tcaccgttaa cggcctggac
    14221 gtgatcgtct acaacgactc cagcccggcg aaccgcggcg gaagcgccgg gcggagccgc
    14281 gtgatcgtgt cgctcgcggg cgtggaggcc gagtcgcgct acgggatcta tctgcttccc
    14341 gacgccggcg agatccccgg cgacgccggg agcgtcgtgg tcttcgatcc cgcgccggac
    14401 atcggcggag cagaccccgc caccgaggat ccgcggccgg agcggccccg gccgggcatg
    14461 tccgccggac cggccggacc ggccggaccg gccgaggcga tcgtgcgctc gtttgcgttc
    14521 gtgcggaacc gccccggcga gttcggcatg ctgttcgtgc tgtggagcct gctcgccgcg
    14581 ccggcctacc tcgcgctccg ccgccgtgcg atgagcaggg tgctggacgt atgagccgca
    14641 ccccgtcgtc gggatcgggc gcgtgggccg cggccgggat cgtgatcgcg atcagcctgc
    14701 tgtcggcatt caccgcccgg ctgcccacgg tcgagcccgt cgtcaatgcg agcgcgccgg
    14761 tcgccgatcc cggcgccgct cccgccggcg gcgaggcccc ggtggccgcg ccggccggca
    14821 gcgacggacc ggtcccggtg ccggccggca gcgcgccggt cacgacggtc ggcggaaagg
    14881 tctacgactg cgcgaaggga cacaacgccg gcgcgaccga cgtgggcgtg acggcgaagg
    14941 agatccgctt cgccgcgacc gtggtcaaga ccggcatcgc caaagacttc ctcgccgacg
    15001 cgcagttcgg catggaggcc gtgcgtcaga aggtcaaccg ccagggaggc gtgtgcgggc
    15061 gcaccatcca gatcacgtat caggacgacg ggtgggagcc ggcacgcggc cagcgcatca
    15121 tcgaaggctg gatccagtcg aagaagtttt tcgggctcgc ggtgaatccg tccagcgaag
    15181 gcgtgcgcgg cgcgatcgac tcgcacgtga tccgcgacgc gaagttcccg gtgatcggct
    15241 ccgatggcca gctgatcgac caataccagg atccgtgggt atggccggtc gcgacctcga
    15301 ccgcgtcggt catgcacatc atggcgcgcc acgcctggca gcaaggcgca cgtcggttcg
    15361 ggatcgtgtg ggatcagaac taccgcttcg gcgtcgaagg ccacaaggcg ttcctcggct
    15421 cgatcaagcg cctcggcggc acggtcacct cggacgtggg cgtgcagggc gggcagcagt
    15481 cgtactcgaa ccaggtcaac gacttcatcg gcagctgcgg cggaacctcg ttcgcccgat
    15541 gtgacttcgt tgcgatgctg ctcgagccgg ccaccgcgac gcgctgggtc ggtgacggcg
    15601 ggctcggcaa cggcaaccag cggccgcgtg ttggcatcgg ggcgccgcag ccgctgttcg
    15661 tggacgcgtt cgcgcgcgac tgcggaaagt actgcgacgg catgctggtg tggacctcgt
    15721 tcaagccgcc gctggacccg ttcgacaagg agccggcggt cgcgacctac ttggccgacc
    15781 tgcggcgcgt gaactcgaag gccgacgcga acaacccgca cgtgcagggc gcttatatcg
    15841 ggatgcaact gctcgtcgaa gcgctcacgc ggctcggccc ggccccgacg cggctcggga
    15901 tcaaggcggt gctcgatacg atgaccttcg agacgggcct ggcgcccccg atgcgcttct
    15961 cgaagggccg cccgcagttc cccaacgtga aggcccaggc gttcgcgtcg ctggcgcaga
    16021 ccggatcgtt ccagggatgg cgctacgcga actccggctt catcgacgac gtcgacgccg
    16081 gcaaggacgc ggcctgacgt gaacgacttc ttcgtcaaca tcatcttgac gatgccgctc
    16141 gtcggcgcct acacgatgtt cgcgctcggg atcgtcttca tctaccgcgg ctctaaggtg
    16201 ctcaacctcg cgcacggcgc gatggcgatg ttcccggcct acgtcgccta ctcggcggcg
    16261 cagcgaacga gtgtgttcgt cgcgctggcc gtcgcgctcg tcgccggcgc gctgctcggg
    16321 ctcggcgttg agcgcatcgt ggtgcggcgg ctccgcccga gcggcccgac cgcgcagacc
    16381 gtcggcaccg tcgcggtcct gggattgctg atcgcgatcg ccgcgcggct gtggggaacg
    16441 acgccgctgc tggcgccgca gatcttcccg aagcagatct atcaggtcgg caacgccacg
    16501 cttcagctcg gatcggtcgg gctgttcgcc gtggccctgg ccgcggccgc cgcgttcatc
    16561 gcgctgttca agtacaccga cctcggcctg gcgctgcgcg cgagcgcgaa caatccgcgc
    16621 gccgcaggcc tggtcggcat cgatcccgag aagaccaccg cggtcgcgtg ggcattcgcg
    16681 ggcgtgctcg cggcgctcgg cggcgtgctg cttgccgcaa ccacgaacct gcatccgtac
    16741 acgctctcgc tcgaggtgct gcccgcgttc gtcgcggctc tgctgggcgg gctcgacgac
    16801 gtcaaaggcg cggtgatcgg ctcgatcttc gtcggcatgg tgatcggcat cgtgccaacg
    16861 ctgccggtga tcggctcaac ggtcggcatc cagcaggccg tgctcggcct cgtcgcggtc
    16921 gtggtgatgg gacttcgcgg ccaacggttc tcggcctcgg acgtgcggag cggcctgtga
    16981 ggctcgcaga gaccgaccgc cgcgtctacc ggatgctggc ccgggccgcc gtcgtcgccg
    17041 cgctgatcct tgcgccgtgg ctgccgccgc tgtcgtcgca gcccgcgctc gtccaggcgg
    17101 cgaacctggc gggcgagtac gcgctgatcg cgatgagcct ggtgatcctg accggctggg
    17161 tcgggcagat ctcgctcggg caaggggcct tcgtcggcat cggcgcgttc accaccggcc
    17221 tgctcgcgcg cgaggccggc atcccgttcc cgatcaacct tccgatcgtc gtcgcggtga
    17281 ccgcggcggt ggcgtcgctg ctggggctcg tcgcgttgcg cgtgcgcggc ctctacctgg
    17341 cggtcgcgac gctgatcttc gcctatgtcg ccgacaagtt cctgttcacc tccgcgtggc
    17401 tggtcggcga aggcggctcg tccacgatcc cgaaccgaac catcggccgt cccggcaccg
    17461 tcacgttctt cgacttccgc tccaaggagg tcttctatct ggtcgtgctc gccgcggtgg
    17521 cggcggcttg gtacgcggcg atgaaccttc gcgagtccaa gaccggccgc gcgttcttcg
    17581 cggtacgcgg ctccgagacc gcggccgtct cgctcggcat cgacgtcacg cgctacaagc
    17641 tgctcgcgtt cgcgctgtcg ggcgcgctcg ccgggatcgc gggcacgctg atgatgaccg
    17701 acctcaagac tgcgacgccg acaggcttcg acttcactcg ctcgctgttc tatctcggca
    17761 tcgccgtcac cggcggcatc ggatcgctcg gcggtgccat cgccgccagc ggcgcgttcg
    17821 cgttcctgaa cgcgatcggc cagatctggg acaccgcgct cacccgcgcg atcgccgaca
    17881 atctcgacat tgtgtcggtc ggtcttttgc tgctggtgct gctggcctat cccgggggtc
    17941 tgggcgcggc cggggccgcc gtggggaagc gattgcggga acgcgcgcga tccgggcgcc
    18001 gcgaaagcgt gtcggacgaa gcgccatccc gccctgcgcg gcagcgcgtt ccgaggcggc
    18061 gcggcgtcga gcggcgagcc ctggactttt cgcatctgga gtccgagccg cgcgacgatg
    18121 agctttccgt gatcggctcg gccgccgcgc agcaggctcg ccgcgattgg cgcaccgccc
    18181 agcccgcgcc gctcgacctt cccgaggacc ggtcgcagcg cgagccgatc ctggaggccc
    18241 gcaacatcgt cgtgcgcttc ggggggctga ccgcggccga cgatgtgacc gtcgaggttc
    18301 gcaaaagcga gatcgtgggg ctcatcggcc cgaacggtgc cggcaagacc acgaccttca
    18361 acgcgatcgc cggattgaac gagccggcga gcggaaccgt gcgcctctac ggccaggatg
    18421 cgacgacgct gccggtgcac gtgcgtgccc agatgggcgt cggccggacc ttccagctca
    18481 tccagctgtt cccgcagctg tcggtgttcg agaacctgct cgtcgccacg catctgcaca
    18541 acgacaccgg cgtgctgcgc cacctcgccc tgaccgacgc cgcgatcgaa gccgagcgcg
    18601 aggcgcggcg atccgtccgc cggatcatcg gcatgctcgg cctggaagac gtcgccgacc
    18661 gccgcgtcgc cggcctcccc ttcggcgtcc tgcgtcacgt cgagctcgcg cgggccctcg
    18721 tgacgcgcgc gccgctgctc atgctcgacg agcccgccag cggtctggac aacaccgaga
    18781 ccgacgagtt cgcccgcttg ctgaagttca tccgggccga gctcggcgtg tcgatcctgc
    18841 tgatcgagca cgatgtccgt atggtgacga gtgtgtgcga ctacatctac gtgatcaacc
    18901 gcggcgcgcc gctcgccgac ggcacgccgt ccgagatcca gcgtcacccc gacgtggtcg
    18961 ccgcctatct gggcgaggcg gtgcacgcat gaagcgcttc gcgctcgttg cgctgatcgc
    19021 gctcgccgcg tgcagcggcg atccgtccgt aggcgacaga gcgtctcccc gagcctcgtc
    19081 gccgccgccg gctgcggcgt cctcgcccgc gaccgcgatg gaggggcgga ccggtgggag
    19141 cccgtcaccc ggcacggctc ccggcgccgg cgtggcgccg agcgcgcagg cgagcgcctc
    19201 caccgtcgcc gcctccctcg ccaggccctg cgtccccgcc ggacagaccc agacgatgac
    19261 ggtgaccggc ctgaagccca acgaggtcac cggctacagc acgcagtact cggacggatc
    19321 gaatgagctg accaacaaaa gctacacgag cggattcggc aacggcgttg ccgggccgaa
    19381 cggccgctac gaaacgacct gggtcgtgcc ggccgccgcg cccgccgggc gcgccgaggt
    19441 gctggtcatc tgggcgtcgg gcacggagcc gcttcgcgta tcgttcatga tcccggccgg
    19501 cgggcagggc tgctgagttg ctcgagatcc gcggcatcga cgtcttctac ggtccgatcc
    19561 aagggctgcg cggggtctcg atgacggtgg gcgaaggtga gcgggtcgcg ctcgtgggcg
    19621 cgaacggcgc cggaaagacc acgacgctgc gtgcgatcag cgggatgctc gctcccgcgg
    19681 ccggcgagat cctgttcgag ggaaccccga tccacgggct caagcccaat caggtcgtcg
    19741 ctcgggggat cgcgcacctg cccgagggcc gcgagctgtt caccgagctc tccgtcgagg
    19801 acaacctcag ggccggctat tggacgcagc gaaagaacgg ctcgttcgcc gctcagcgcg
    19861 accgggtgat ggatcacttt ccgattcttc gcgaacgcgc cgcgcaggcc gccggcacgc
    19921 tgtcgggtgg cgagcagcag atgctcggcg tggcgcgcgc gctgatgtcg aacccgcgcc
    19981 tgctgatcgt cgacgagctc tcgctcggct tggcacccaa gatcgtgtcg cagctgttcg
    20041 acatcctgcg cgaggtcaac gttgcaggct gcgccgtcct gatcgtcgag cagttcgtgc
    20101 acatggcgct cgccaacacc gaccgtgcct acgtgctcgc caaaggcgag gtggtgctgg
    20161 gaggcgccag cgcggacctg gccgccgacc cgaagctggt cgcgtcctat ctcggcgacg
    20221 acgagcccga cgacgagccc aacgacgagc cgaagcggag aggagcacgc aagcggtgag
    20281 cccggctcgc agcgagcgat ggggttcgcg caagcgccgc cgccggatcg tgcgcatcgt
    20341 cgtggcggtg atcgttatcg cgggcgtggt cgctgcgatc gtcgttcccc gatcgcgcgc
    20401 cagccgccgc gccgaacgcg ccgcggttca gacgtacgcg caagtcgtca aggatttgtc
    20461 tttcgagggc ggccgcatcc ttcagcaaga ggtcaagccg cgcatcaccg atctgcgcga
    20521 aggcgtcgtg tctcccgagc agttccgccg cgaggccgac aactggatcc ggctctacca
    20581 gaacctgcga aaaagcttcg ccgacgccgg ccctcccgag cggctcgcca cggccgcgcg
    20641 gctctacgac gatgcgttcg ttgcctacat cgaagccttc gaggcattcc gtgaggcgag
    20701 ccggctcgac acccccgagg cccgcaacga ggcgatcacc gaagccgtgc cgatcgccga
    20761 acgcgcggac cagatcttcg accgtgcccg cggcgagctc ggggagcaga tgcgcgcgct
    20821 cgggctgacg cccccggggg atctgtagga gcgccggcga gccggacgaa ggcccggccc
    20881 gccggcgtat ccggaggatt cggcgcggca gtgcggccac cacgccggac tcggtgagtc
    20941 ggctccgcat cgcgtccggt cagccaggcc cgaacgcgcg gcaggcgaaa ccgcctccct
    21001 cgtcacgact ccgacccggg agcgggccga tcctttcagc tagaggttgc cgaggatctg
    21061 gtaccagtac ttttggtcct tgcccggcgt ctgatccacg tggatgtact tcgtctgctg
    21121 gtactcgcgc aggccccagg cgcccagctc gcggccgacg ccgctctgct tgtagccgcc
    21181 gaacggcgcg taggcgctga gcaggtggta gtcgttgatc cagaccgtgc cggtgcggat
    21241 gcgcttggcg acgttgatcg cgcgcggcac gtcccgtgac cacacggccc cgcccaatcc
    21301 gtagatcgag tcgttggcca tcttgatcgc gtcctcgacc gagtcgtact tgatgaccga
    21361 caggaccggc ccgaagatct cctcctgcgc gatgcgcatc gagttctcga cgcccgtgaa
    21421 gatcgtcggc ttgtagtagt agccgccctc gtggccctcg acgtcgacgc gcgcaccgcc
    21481 ggtcgcgagc ttcgcgccct cctcaaggcc gagctgcacg tagcgctcga ccgtctcgta
    21541 ctgggtgcgg tggatcagcg gacccatgtc ggtctcgaag ctcatcgcgc tgcccaccgt
    21601 gagcgactcg gccgcatcca ccagccggga cacgatctcg tcgtggatgg aggcgggcac
    21661 gaacaagcgc gtgcccgagt gacagatctg gccggagtgc aggaacgtgc cgaacagcga
    21721 gccgggaacc gcggcgtcca ggtccgcgtc gtcgaggatg atgttggcgc tcttgccgcc
    21781 gagttcgagc gtcgtcttct tcaccgtgct cgacgcgagc tgcatgatcc ggcggccgac
    21841 ctcggtcgat ccggtgaatg cgaccttgtc gatcatcggg ttggatgcga gctcctcgcc
    21901 ggccgcgccg cccgcgccgg gaaccacgtt gagcacaccc ggcggcagga cgtcggcttc
    21961 ggcgaccagc ttggcgaact cgacggtgga gcatggcgtg taagtcgcgg gcttgaccac
    22021 gacgctgttg ccggtcgcca gcgccggcgc gaccttccac atcatcagga tgaacgggaa
    22081 gttccacggg gtgatcgccg cggtgacgcc gtagggctcg cggcgcacca gccccatcgc
    22141 gaaggcgggc atctcgttga acggcaccgg ctcccactcg cccagcttgt tcgccaggtc
    22201 cgcgagcgcg cgaaggtgct ccacgccgag cgcgatcgag aacaggttcg ccatgcgcag
    22261 cgtgtgcccc gcatccatcg cctcgatgtt cgaaagatca ccgagattgt ccacaagcag
    22321 cttccacagc ttcagcagcc gctcgctccg ctcggcctgc ggcatctcgg accacacgcc
    22381 cgagtcgaac gcggtccgcg cggcttcgat gccccggcgc atgtcctccc tgccgccctt
    22441 ggcgaccgtg gcgaacagag atccgtccga cgggttgtag gtctcgaacg tcgcgccgtc
    22501 cgcggcatcg acgaactcgc cgttgatgta gagctgatag ctgcgtacgt cgctcatccg
    22561 tggtcccctc ccgtcgcgcc gagcggcgcc gttggccccc gattccggtt ccgaaatcct
    22621 acccttcggt cgcgcggggg cggtagcccg ctccgctgcc gtttgcgcga gcctcgcgtc
    22681 ggccgcttaa taggggccga tgagacgggt tcgagtcgcg atcgcgatag cgcttgccgt
    22741 agtgccggct gcagcctggc tggcgttcgc cccgccggcc ccggcggccg agcgcagtgt
    22801 gcgtgtggtc acgggcaatc gcttcgatcc caaggacaag ttcgtcgtcg ccggagacac
    22861 ggtcgtgtgg acctacgatg atgcgaacgg cgcgccgcac accgtcacgc gcgagaccac
    22921 gccggagcgc ttcgactcca gcccgagcaa ctgtccccgg accaatccgc tcgagcccga
    22981 cgactgcctg gactccaaca accggaccta ctcgcatcga ttcgaccggc ccggcaccta
    23041 ctcgtacttc tgcaaggtcc acggccagag catgagcggc accatccacg tcgcgccgcg
    23101 cgagacggcg gcgccttctc cctcaccgac ccggacgccg gcctcgccgt ccccatcgcc
    23161 gtccgggtcg ccgtccggat caggctctcc cgacccgagc gcgtccccca ccggcacgtc
    23221 cacggcctcg ccgtcgcccg aagacacgct cacgcccacg ccgagcgaca ccggcacgcc
    23281 ggctccgtcg ccgggtggcg gcagcggcac caaggttgcg ctcgcggtcg tcgcgctcgg
    23341 atcgctcggc ggcgccggct tcctggtcta tcgccgcttc ctgtcctagc tgcgaaagtg
    23401 catcggggac ggggcgcccc tatgattcgg caccgtcatg aagtggctca cccgctcgat
    23461 catcccttcg atggccgctc tggccgcgct gatcgcgttc ctgcccagga ccgggccggc
    23521 ggtcgggttg gtcaccggcg atttcgcctt cacgccgcag gcgctcgtcg tcgagcaggg
    23581 cgaggcgatc accatccgca acgacgggga cctgacgcac tcgttcacct gcccggagtg
    23641 cggcatgcgc agcatcaaca tccagcccgg ccaggccaag atcgccacct tcccgacggc
    23701 gggaactttc gagttcttct gcgtctacca tcgcaccgaa ggcatggagg gccgggtgac
    23761 cgtagaaggc gacgcgcccg cggcccccga gccggcaggg tcgcctacgc ccgacccttc
    23821 gccgtagcta ctcgcccgac ggccgcagcg gactgggcga cggggacggt gacggaaccg
    23881 tcgtcgggga cggcatcggg gtcggggtcg gggtcgccgt tgcggtggcc gtggcggtga
    23941 cggtcgggga t
//
LOCUS       DATLFO010000361        10139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_146968, whole genome shotgun sequence.
ACCESSION   DATLFO010000361 DATLFO010000000
VERSION     DATLFO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146968"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1298
                     /locus_tag="VM841_14750"
     CDS             237..1298
                     /locus_tag="VM841_14750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HVL91484.1"
                     /translation="MEPLNLNEFEAVAREILPRNAYDYYAGGSDDEITLAENRDAFAR
                     RSLKYRVLVDVAERDLSTRLLDSILPHPILLAPTALHKMAHPEGEVATARGAEAAGAL
                     QCVSTISTVPLEQVMEAAPRSPKWFQLYVYSDRAVTESLVKRAYAAGYRAIVLTVDLP
                     VLGRRERDFRNVFTIPEGITLANFAAPPPRPEGGSALASWASDLSTHSLTWSDIAWLR
                     SLSPLPIILKGVVRGDDAIRAADMGVESIWVSNHGGRQLDTSVATLDALEEIADATEG
                     RVPLIVDGGVRRGTDVLKAIALGASAVAIGRPQLWALAAGGAGGVQRAIDMLREELSI
                     AMALAGCPSLSEITSDLIA"
     gene            1331..1795
                     /locus_tag="VM841_14755"
     CDS             1331..1795
                     /locus_tag="VM841_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91485.1"
                     /translation="MFNRPGWQTSMALAFIAGGFIVLFIAWNGAASIDFVQGQVPYLL
                     SGGFVGLGMIIVGVGLMLFESGRRAGTKVDRKMDELSEVIKTIQVSSNGSSKDHAMAE
                     ATFADGKVVVGARSYHRPDCRLVEGKDALVYATQPLAAEQGLKPCRVCNPED"
     gene            complement(1792..2964)
                     /locus_tag="VM841_14760"
     CDS             complement(1792..2964)
                     /locus_tag="VM841_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVL91486.1"
                     /translation="MSDVRRILLLRGIRSFAYGFTSVLVGVHLADLVSAPRAGAILTA
                     TLAGAAYWTFVVGARGDRIGRRRMYVALSAIMAVAMVVFAVSDAFAALLIASLSGTVA
                     ATALEAGPFVALEQAMIPGAVEAAHRTRTFGRYNSVAAIVGAFGALAAGGPAALRDVL
                     PVASQRLFVVPALLALSSLVVALRLSDAVEAPIRGAKPLTVSRGRVAKLSALFALDSF
                     GGGFVVQAAIVYWFRSRYGTSEEMLGLVFFGTGMLQAVSFAIATRLADRIGLINTMVF
                     THLPSNLLLAAVPLAPNEPLAITLLLGRFALSQMDVPVRQSYVMAVVEPEERTAAAGF
                     TTTVRSAAQAAGPAIGGATIPIAGSGLPFFVGGGLKVIYDVLLWINFRRVKPPEES"
     gene            complement(2980..3687)
                     /locus_tag="VM841_14765"
     CDS             complement(2980..3687)
                     /locus_tag="VM841_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL91487.1"
                     /translation="MPLLEIVDLRTGYGALPVLHGISMEIEAGDTAVMLGLNGAGKST
                     TMMALAGLLPTWAGQINFEGENIAKMDTGSRVRKGIVLVPEGRRVFPALPVHVNLRLG
                     NWTHRKDKRKQKELMERVFEMFPRLAERRNQLAGTMSGGEQQMLAVGRGLMADPKVLL
                     IDEASLGLAPKTAQELFETARGIAATGVAVILVEQNAGALKVADRAWIMQKGTLVFTG
                     YGDELRSGVDLRAAYLG"
     gene            complement(3674..4501)
                     /locus_tag="VM841_14770"
     CDS             complement(3674..4501)
                     /locus_tag="VM841_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVL91488.1"
                     /translation="MSVPETFPQQPAARGQGGAMLEVRDLGIRFGGLQALGGVNVDVN
                     EWEIVGLIGPNGAGKTTFFNCVMGVYAPTTGSVRYRDKEITTLPTHRRTTLGLGRTFQ
                     NVGLAKRLTVMENMLIAQHTKAAYGVVPAVFGTPAVLAEEKLLRRNAFEILDFMGLAD
                     HHGEIVSSLPYGLQKLSEIAAVLAGDPEFLLLDEPTSGMSPEEAHVFGDLLLRLRTDL
                     NLTVLMIEHHVPLVVRVCDYVYVLNFGQLLAEGLPQQIQRHPEVVAAYLGEDADAAS"
     gene            complement(4488..5684)
                     /locus_tag="VM841_14775"
     CDS             complement(4488..5684)
                     /locus_tag="VM841_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL91489.1"
                     /translation="MAPSRRNEKPVAVTPPAQAPTPVAEIVAEEETQALGRVEVSKPT
                     PWTPSKRGWIARRVVTALLALIVIGSPALVGNIENLRAISLAMIYVIVGLSINILMGY
                     AGQISLGHQAFVGVGAFTAALVVTKASAPFFVALLLAGVIGAISAFLLGIVALRLKGL
                     YLALITLAYGAVAEFTIFATPAITGGGAGVNAVRPSFLVSERSFVYFSMLMVAVALYI
                     DWRLVRSKVGRAIFAIRENELAAASFGINVTSYKLVAFVISGIFAGVAGALFGFRVES
                     VDAANFDFALGLTFVLMAVVGGLGSRIGVFIGSAFFALLDLLIHSLFGTGFGHWLVDK
                     VPFLGQLELYPGFIGAVLLLMTLTLYPGGIAQQIKPLTGWLGGKRLKDHGHGVTIREG
                     GAGVRP"
     gene            complement(5684..6568)
                     /locus_tag="VM841_14780"
     CDS             complement(5684..6568)
                     /locus_tag="VM841_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVL91490.1"
                     /translation="MTSAIILGIIQGAVYGLVALGLVLVYKGARVFNFAQAEYGTIGT
                     FFAWFLIDRKGVPYLFAVVLAIAGVALVGFLIERLIVRPLFDAPRVTMLVATAGVALA
                     AIQLQLIATGPNVQSLSPAVEGFGPSILGVQVSPQRILIFVVLAGIGAAMAYFFNKTD
                     LGLAVLATSQEPTATELAGISTKRISSFIWTLAAVLGAVAGILFAPSIGAFAPGYMTT
                     AVLLPAFTAALVGGITSLPGAFVGGQIVGITQQIGQYLDGRYELGRFIVSMPTLLVFV
                     VLVTILLVRPKGLLGSDA"
     gene            complement(6581..8107)
                     /locus_tag="VM841_14785"
     CDS             complement(6581..8107)
                     /locus_tag="VM841_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVL91491.1"
                     /translation="MRTRPLIAFLAVLALTLTACGQKPGVHVSIRDGGGNGGIDGGGT
                     DSDGDGIVDPIDGGGSGDGGSGNDGGGNDGGGNNNGGGNNGGGGNPDGGGNNGGGGII
                     GSDRTGITADTITIAIHAPTSGAAPIEPKAFANGSDTYWKWLESQGKRIHGRKVRIFF
                     RDDKYKPSSAVDACTALTTGKDAAFLLIGAAGTDQIRACADYANRKGIPYFSAGVQES
                     VVKGYKTYFALTMTYAQQMPLLMQRIAVEGNALDQWTTGGVKRGDGKIKIAFVRPNTS
                     NFDDAQRAFEAAFAKLDQSKYQKTIKTVIKEGTQSEAAQLAQTLKNEGVDVVSPITAP
                     NFTVYLAGAAGGQQYNPRYMGVGVTNGVNQGIARMCTNQDFHNAVFFSPWPAWSHRSQ
                     AGIGDPNFERAVQQDDAQQVNGRNNGGDLLLALWGINRTIHGILEAAGPNVTRESLVA
                     MMRTYKGGTSGFFPDLSYSSGNPFGAQGVHSLVGRCDANGSSGEFIEDPKYLGLRKSF
                     "
     gene            complement(8114..9277)
                     /locus_tag="VM841_14790"
     CDS             complement(8114..9277)
                     /locus_tag="VM841_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91492.1"
                     /translation="MTPKFVVAFRTRAVRAASVAIIAVLGAMLWAPTALAISATESIE
                     MIQENQGWYWSSNREFRQCVGTDDTGLCYVNDLSGAAPVEPGHLAVALQMGGSDMRSY
                     LIFPLFSVPEDATVSAMTLTLPVSRTDAADQEHTTHHTGPGAKAPSTTNEARASITAC
                     AVTVPWGAVEGAPSYAMDTKDPRQTEPVEPFVKDGVDCAKWSVKGKAGEKAWTFDLTK
                     IAQMWVSGALDNNGVVLLPDLTGGTDSWRIEFHGAPVEVQNNDTRQTYVSPKEAGSTT
                     ISYTAPDATPEPTPEPTGDGGGFIPPPPATFEPAPEPAPTSTDDPQPVQPADPEPVGE
                     TATPTAWYAWLALPLGAVAHAGLARAVGREAGEAGANRVATMLRRRRLERAGE"
     gene            complement(9320..9931)
                     /locus_tag="VM841_14795"
     CDS             complement(9320..9931)
                     /locus_tag="VM841_14795"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVL91493.1"
                     /translation="MEPIPWFVEPVRAALAATPSTTMPREGSRPSAVLLMLYDHGGEP
                     GLVFTKRSELVRHHKGEISFPGGGMEPGDRSLEDTAIRETVEELGVDPAEITIIGDMA
                     EMLTYVTGFLVKPYVAHVPARKVYAHSEAEIAEVLRVPLRTLVEVGREAEWEARGYRF
                     TTNVFEVGPNVIWGATGKILRDFLDVVAPALELPWPRPAGTTR"
BASE COUNT         1638 a         3543 c         3380 g         1578 t
ORIGIN      
        1 atccgttctc gacgccggag ctgctcgacg cgaccatcgc gaagctcccc ctcgcaacgc
       61 tctccccgat cgatggcggc gatcattcgt tcaaggtctc cgggcgcgat cccgccgacg
      121 tcaccgaaga gctgatcgag cgcaccggca cgttcatcga ccgcatcctg gccgggtagc
      181 ggagttcggc gatctcttcg ctcgctaggc gagggcttgg taggctcggc gccgccatgg
      241 agccgctcaa cctcaacgaa ttcgaagccg tcgcccgcga gatcctgccg cgcaacgcct
      301 acgactacta cgcgggcggc tccgacgacg agatcaccct cgccgagaac cgcgacgcct
      361 tcgcgcggcg ctcgctgaaa taccgggtgc tagtggacgt cgcggagcgc gacctgtcca
      421 cgcgcctgct ggactcgatc ctgccgcatc cgatcctcct cgcaccgacc gcgctgcaca
      481 agatggcgca tcccgagggt gaagtcgcga ccgcgcgcgg cgccgaagcc gcgggcgcac
      541 tgcaatgcgt ctcgaccatc tcgaccgttc cgctcgagca ggtgatggag gcggcaccgc
      601 ggtcgccgaa atggttccag ctctacgtct attccgaccg cgccgtcacc gagtcgctgg
      661 tcaagcgcgc ctatgccgcc ggctaccgcg cgatcgtgct gaccgtggac cttccggtgc
      721 tcggccgccg cgaacgcgac ttccgcaacg tgttcacgat ccccgaaggc atcacgctcg
      781 ccaacttcgc cgcgccgccg ccgcgtcccg aaggcggcag cgcgctcgcc tcgtgggcgt
      841 cggacctgtc cacgcactcg ctgacatgga gcgacatcgc gtggctgcgt tcgctgtcgc
      901 cgctgccgat catcttgaaa ggcgtcgtgc gcggcgacga cgcgattcgc gccgccgaca
      961 tgggcgtgga atcgatctgg gtctccaatc acggcggccg ccagctcgac acctcggtcg
     1021 cgacgctgga cgcgctggag gagatcgccg acgcgaccga gggacgcgtg ccgctgatcg
     1081 tggacggcgg cgtccgccgc ggcaccgacg ttttgaaggc gatcgcgctg ggcgcctcgg
     1141 cggtggcgat cggacggccg cagctgtggg cgctggccgc cggcggcgcc ggcggcgtgc
     1201 agcgagcgat cgacatgctt cgcgaagagc tttcgatcgc gatggcgctg gcgggctgcc
     1261 cctcactgtc cgagatcact tcggacttga tcgcctagca cgccccgccc ggctccgata
     1321 ccatccgggc atgttcaatc gtcccggctg gcagacctcg atggcgctcg cattcatcgc
     1381 gggcggtttc atcgttttgt tcatcgcctg gaacggcgcg gcttcaatcg acttcgtgca
     1441 gggccaggtc ccctacctgc tgtccggcgg cttcgtcggg ctggggatga tcatcgtggg
     1501 cgtgggcttg atgctgttcg agtcgggccg ccgggccggc accaaagtgg accgcaaaat
     1561 ggacgagctg agcgaggtca tcaagacgat ccaggtctcg tcgaacggat cctccaaaga
     1621 ccacgcgatg gccgaggcca ccttcgccga cggcaaggtc gtcgtcggcg cccgctcgta
     1681 tcaccgcccc gactgccggc tggtcgaagg caaagacgcg ctggtctacg cgacccagcc
     1741 gctcgcggcc gagcagggcc tcaagccctg ccgggtctgc aaccccgaag actaggattc
     1801 ttcgggcggc ttcacgcggc ggaagttgat ccacagcagc acgtcgtaga tcaccttcag
     1861 cccgcctccc acgaagaacg gcagcccgct gccggcgatc gggatcgtcg caccgccgat
     1921 tgcgggaccg gccgcctgcg cggcgctgcg gaccgtggtg gtgaagccgg ccgcggcggt
     1981 gcgctcttcc ggctcgacga cggccatgac gtaggactgg cgaacgggca cgtccatctg
     2041 cgacagcgcg aaccggccca gcagcagcgt tatcgccagc ggctcgttcg gggcgagcgg
     2101 cacggcggcg agcagcaggt tcgacggaag atgcgtgaac accatcgtat tgatcagccc
     2161 gatccgatcg gccagccgcg tcgcgatcgc gaacgagacg gcctgcagca tgccggtgcc
     2221 gaagaagacg agcccgagca tctcttccga tgttccgtac cggctgcgga accagtagac
     2281 gatcgcggcc tgcacgacga agccgccgcc gaacgagtcg agcgcgaaca gcgccgacag
     2341 cttcgcgacc cggccgcggc tgaccgtcag cggtttcgcg ccgcggatcg gcgcttccac
     2401 cgcgtcgctc aggcgcagcg cgaccaccag cgagctcagc gccagcagcg ccggcacgac
     2461 gaacaatcgc tgcgacgcga ccggcagcac gtcgcgcagc gccgcgggac cgccggccgc
     2521 cagcgcgccg aacgccccca cgatcgccgc gaccgagttg tagcggccga acgtgcgcgt
     2581 gcgatgcgcg gcttcgacgg cgcccgggat catcgcctgc tccagcgcga cgaacgggcc
     2641 ggcctccaac gcggttgcgg cgacggtgcc cgacagcgat gcaatcagca gcgcggcaaa
     2701 tgcgtcgctg acggcgaaca ccaccatcgc gacggccatg atcgcgctca gcgcgacgta
     2761 catccgccgc cgcccgatcc ggtcgccgcg cgcgccgacg acgaaggtcc aatacgccgc
     2821 accggccagc gtcgcggtca agatcgcgcc cgcgcgcggc gccgacacca gatccgccag
     2881 atgcaccccg accagcaccg aggtgaaccc gtacgcgaag gatcggatcc ctcgcagcag
     2941 caggatgcgc cgcacgtcgc tcatgcgacg actgcggcct cagccgaggt aggcggcgcg
     3001 caagtccacg ccgctgcgca gctcgtcgcc gtagccggtg aagaccagcg tgcccttctg
     3061 catgatccag gcgcggtcgg cgaccttgag cgcgcccgcg ttctgctcga ccaggatcac
     3121 cgcgaccccg gtcgcggcga tgccgcgcgc ggtctcgaac agctcttgcg cggtcttggg
     3181 cgcaaggccc aacgacgctt cgtcgatgag cagcaccttc gggtcggcca tcaggccgcg
     3241 gccgacggcc agcatctgct gctcgccgcc cgacatcgtg ccggcgagct ggttgcggcg
     3301 ctcggccaaa cgcggaaaca tctcgaagac ccgctccatc agctctttct gcttgcgctt
     3361 gtccttgcgg tgcgtccagt tgccgagccg caggttcacg tgcaccggaa gcgccgggaa
     3421 cacgcgccgg ccttccggca ccagcacaat gcctttgcgc acgcgcgagc cagtgtccat
     3481 cttggcgatg ttctcgcctt cgaagttgat ctgtcccgcc caggtcggca gcaggccggc
     3541 gagcgccatc atcgtggtgg acttgccggc gccgttcagg ccgagcatca ccgcggtgtc
     3601 gcctgcctcg atctccatcg agatgccgtg cagaaccgga agcgcgccgt agccggtgcg
     3661 aaggtcgacg atctcaagaa gcggcatcgg cgtcctctcc cagataggcg gccacgacct
     3721 cgggatgccg ctggatctgc tgcggcaatc cttcggccag cagctggccg aagttgagca
     3781 cgtacacgta gtcgcagacc cgcacgacca gcggcacgtg atgctcgatc atcagcacgg
     3841 tcaggttcag gtcggtgcgc agccgtagca gcaggtcgcc gaacacgtgc gcctcttccg
     3901 ggctcatgcc cgaggtcggc tcgtccagca gcaaaaactc ggggtcgccg gccagcacgg
     3961 ccgcgatctc ggagagcttc tgcaggccgt agggcaacga cgacacgatc tcgccgtgat
     4021 gatcggccaa gcccatgaag tccaggatct cgaatgcgtt gcgccgcagc agcttctctt
     4081 cggccaagac cgcgggcgtg ccgaataccg ccggcacgac gccataggct gctttggtgt
     4141 gctgggcgat cagcatgttc tccatcaccg tcagccgctt ggccagcccc acgttctgga
     4201 acgtacggcc cagtccgagc gtggtgcggc gatgcgtcgg cagcgtcgtg atttccttgt
     4261 cgcggtagcg caccgagccg gtcgtgggcg cgtagacgcc catcacgcag ttgaagaacg
     4321 tggtcttgcc ggcgccgttg gggccgatca ggccgacgat ctcccactcg ttgacgtcca
     4381 cgttgacgcc gcccagcgcc tgcaggccgc cgaagcggat ccccaggtcg cgcacttcca
     4441 gcatcgcgcc gccctgaccg cgcgccgcgg gctgctgggg gaaggtctca gggacggaca
     4501 ccggcgcctc cttcccggat cgtgacgccg tggccgtgat ccttgagccg cttgccgccc
     4561 agccacccgg tgagcggttt gatctgctgc gcgatcccgc ccggatacag cgtcagcgtc
     4621 atcagcagca gcaccgcgcc gatgaagccc ggatacagct cgagctgccc gaggaacgga
     4681 accttgtcca ccagccagtg tccgaatccg gtgccgaaca gcgagtggat caaaaggtcc
     4741 agcagcgcga agaacgccga gccgatgaac acgccgatgc gcgagccgag cccgccgacc
     4801 acggccatca gcacgaacgt cagccccagc gcgaagtcga agttcgcggc atccaccgac
     4861 tcgacccgaa agccgaacag cgcgcccgcg accccggcga agatgcccga gatcacgaaa
     4921 gcgacgagct tatagctggt gacgttgatg ccgaacgacg ccgcggccag ctcgttctcc
     4981 cggatcgcga agatcgcccg gccgaccttg gatctgacca gccgccagtc gatgtagagc
     5041 gcgaccgcga ccatcagcat cgagaaatac acgaacgacc gttcgctgac caggaacgag
     5101 ggccggaccg cgttgacccc ggcgccgccg ccggtgatcg cgggcgtcgc gaagatggtg
     5161 aactcggcca cggcgccgta ggcgagcgtg atcagcgcca gatacaagcc tttcaatcgc
     5221 aacgccacga ttcccagaag gaatgccgag atcgcaccga tgacgccggc cagcagcagc
     5281 gcgacgaaga acggcgccga ggccttcgtc acgaccagcg ccgcggtgaa cgcgccgacc
     5341 ccgacgaagg cctgatgccc gagcgagatc tgacctgcat agcccatcaa gatgttgatc
     5401 gacagtccga cgatcacgta gatcatcgcc agcgagatcg cgcgcaggtt ctcgatgttg
     5461 ccgaccagcg cgggcgatcc gatcacgatc agcgcgagaa gcgcggtcac gacgcggcgc
     5521 gcgatccacc ctcgcttcga cggcgtccac ggcgtcggct tcgatacctc gacgcgtccg
     5581 agcgcctggg tctcttcctc ggcgacgatc tcggccaccg gcgtgggcgc ctgcgcgggg
     5641 ggcgtgaccg cgaccggctt ttcgttgcgg cgcgagggtg ccatcaggcg tcgctcccga
     5701 gcaggccctt gggacggacc agaagaatgg tcaccagcac cacgaagacc agcaacgtcg
     5761 gcatcgacac gatgaagcgc ccgagttcgt agcggccgtc cagatactgg ccgatctgct
     5821 gggtgatgcc cacgatctgc ccgccgacga acgcaccggg cagggatgtg atgccgccga
     5881 ccagcgcggc cgtgaacgcg ggcaacagca cggccgtggt catgtagccg ggggcgaacg
     5941 cgccgatcga gggcgcgaac aggatgccgg cgaccgcgcc cagcaccgcc gccagcgtcc
     6001 agatgaacga tgagattcgc ttggtcgaga tgcccgccag ctcggtcgcg gtcggctcct
     6061 gcgaggtcgc cagcaccgcc aggcccaggt cggtcttgtt gaagaagtac gccatcgcgg
     6121 cgccgatccc ggccagcacc acgaagatca ggatccgctg cggcgagacc tgcacgccca
     6181 ggatgctcgg cccgaagcct tcgaccgcgg gcgagagcga ctgcacgttc gggccggtcg
     6241 cgatcagctg aagctggatc gcggcgagcg cgacgcccgc ggtcgcaacc agcatggtca
     6301 cgcgcggcgc gtcgaacagc ggccgcacga tgagccgctc gatcaaaaac ccgacgagcg
     6361 ccacgcccgc gatcgccagc accaccgcga acaggtacgg cacgcccttg cggtcgatca
     6421 ggaaccaggc gaagaaggtg ccgatcgtgc cgtactcggc ctgggcgaag ttgaacacgc
     6481 gcgcgccctt gtagaccagc acgaggccga gcgcgaccaa gccgtagacg gcaccctgga
     6541 tgatgccgag gatgatcgcg gacgtcacga gcctgctccg ctagaagctc ttgcgcaagc
     6601 ccaagtactt gggatcttca atgaactccc ccgaactgcc gttcgcgtcg cagcggccca
     6661 cgagcgaatg cacaccctgc gcgccgaacg ggttgcctga gctgtaggac agatcgggga
     6721 agaaaccgga ggtgccgccc ttataggtgc gcatcatcgc cacgaggctt tcgcgggtca
     6781 cgttgggacc cgcggcctcg aggatgccgt ggatggttcg gttgatgccc cacagcgcca
     6841 gcagcaggtc tcctccgttg ttgcggccgt tgacctgctg ggcgtcgtcc tgctggacgg
     6901 cccgctcgaa gttcgggtcg ccgatgccgg cttggctgcg gtgggaccac gccggccaag
     6961 gggagaagaa cacggcgttg tggaagtcct ggttcgtgca catgcgcgcg atgccctggt
     7021 tcacgccgtt ggtgacgccc acgcccatgt aacgcgggtt gtactgctgt cctccggccg
     7081 cgcccgcgag atagacggtg aagttcgggg ccgtgatcgg cgagaccacg tccacgccct
     7141 cgttcttgag cgtctgcgcg agctgcgcgg cctcggactg ggtgccttcc ttgatcacgg
     7201 tcttgatcgt cttctggtac ttcgactggt cgagcttggc gaaagcggcc tcgaacgcgc
     7261 gctgcgcgtc gtcgaagttg gacgtgttcg gccggacgaa agcgatcttg atcttgccgt
     7321 cgccgcgctt aaccccaccg gtcgtccact ggtccagcgc gttgccctcg acggcgatgc
     7381 gctgcatcag cagcggcatc tgctgcgcgt aggtcatcgt cagcgcgaag taggtcttgt
     7441 agcccttgac gaccgactcc tgcacgccgg cgctgaagta cgggatgcct ttgcgattcg
     7501 cgtagtcggc gcaagcgcgg atctggtcgg tgcccgccgc gccgatcagc aggaaggcgg
     7561 cgtccttgcc ggtcgtgagc gcggtgcagg cgtccacggc gctcgacggc ttgtacttgt
     7621 catcgcggaa gaagatgcgg accttgcgtc cgtggatgcg ctttccctgg gactcgagcc
     7681 acttccaata ggtgtcggag ccgttcgcga acgccttcgg ctcgatcggg gccgcgccgg
     7741 atgtcggcgc gtggatcgcg atcgtgatcg tgtcggccgt gatgccggtg cggtccgagc
     7801 cgatgatccc gccgccaccg ttattgcctc cgccatcggg gttgccgcca ccgccgttgt
     7861 tgccgccgcc gttgttgttg ccgccgccgt cgttgccgcc gccgtcgttg ccggagccgc
     7921 cgtcgccgga gccgccgccg tcgatcgggt ccacgatgcc gtcaccgtcg ctgtcggtgc
     7981 cgccgccgtc gatgccgccg ttgccaccgc cgtcgcggat gctcacgtgg acgcccggct
     8041 tctggccgca cgcggtcagg gtcagggcca ggacggcgag aaatgcgatc agggggcgtg
     8101 tgcgcatcga actctactct ccagctcgtt ccagtcgtct gcgtcgcagc atcgtcgcga
     8161 cgcgattggc tcccgcttcg ccggcttcac ggccgacagc gcgcgccagg cccgcatggg
     8221 cgaccgcgcc cagcggcaag gcaagccatg cataccacgc ggtcggggtg gccgtctcgc
     8281 cgacgggctc cggatcggcc ggctgcaccg gttgcggatc gtccgtagac gtcggcgccg
     8341 gctcgggcgc gggctcgaag gtcgccggcg ggggcggaat gaatcctccg ccgtcgccgg
     8401 tgggctcggg cgtcggctcg ggcgtggcgt cgggcgcggt gtaggagatc gtcgtcgatc
     8461 ccgcttcctt cggagacacg tacgtctggc gcgtgtcgtt gttctgaacc tcgaccggcg
     8521 cgccgtggaa ctcgatgcgc cacgagtcgg tgccgcccgt caggtccggc agcagcacga
     8581 cgccgttatt gtcgagcgcg cccgaaaccc acatctgcgc gatcttggtc agatcgaagg
     8641 tccaggcctt ctcgccggcc ttgcctttca cgctccactt cgcgcagtcg acgccgtcct
     8701 tgacgaaggg ttcgacgggc tcggtctgtc gcggatcctt cgtgtccatc gcgtaggacg
     8761 gcgcgccttc gaccgcgccc cagggcacgg tcaccgcgca ggcggtgatc gaggcgcgcg
     8821 cctcgttggt cgtcgatggc gccttggcac ccggaccggt gtggtgcgtg gtgtgctctt
     8881 ggtcggccgc gtcggtgcgc gagacgggca acgtcagcgt catcgcgctc accgtcgcgt
     8941 cctcgggcac cgaaaacagc gggaagatca ggtagctgcg catgtccgag ccgcccatct
     9001 gcagcgcgac cgcgagatga cccggctcga ccggagcggc gccggaaaga tcgttgacgt
     9061 agcacaggcc cgtgtcgtcg gttcccacgc actggcggaa ctcgcggttc gaggaccagt
     9121 accagccctg gttctcctgg atcatctcga tggattccgt cgcgctgatc gcgagcgcgg
     9181 tcggcgccca cagcatcgcg cccagcacgg cgatgatcgc gaccgacgcc gcgcggacgg
     9241 cccttgtgcg gaaggctacg acgaatttgg gggtcacgat gggcagtcta gcgcggcttc
     9301 tgccctcttc gcccgccctc tagcgagtcg ttccggcggg gcgcggccac ggcagctcga
     9361 gcgcgggcgc gaccacgtcg aggaagtcac gcaggatctt tccggtcgct ccccagatga
     9421 cgttggggcc tacctcgaac acattggtcg tgaaccgata gccgcgcgct tcccactcgg
     9481 cctcgcggcc gacctcaacc agcgtgcgca gcggcacgcg cagcacctcg gcgatctcgg
     9541 cctcgctgtg ggcgtagacc tttcgggcgg gcacgtgcgc gacgtagggc ttgaccagaa
     9601 atcctgtgac atacgtgagc atctcggcca tatcgccgat gatcgtgatc tcggcgggat
     9661 cgacgccgag ctcctcgacg gtctcgcgga tcgcggtgtc ctccagcgag cgatcccccg
     9721 gctccatgcc cccaccagga aacgagatct cgcccttgtg gtggcggacg agctcggacc
     9781 gcttcgtgaa gaccagcccc ggctcgccgc cgtggtcgta gagcatcagc agaaccgccg
     9841 acggacgcga gccctcccgc ggcatcgtgg tgctcggcgt cgccgccagg gcggcgcgaa
     9901 cgggctcgac gaaccatggg ataggctcca cgacggtcac tgtacccaaa ccgagcgggg
     9961 gctttgatgt cgcggtttcc gatcgatcag gacttcgtcg ttcaagtcgg tcggctcgcc
    10021 cacgatcgcc gcgtccgcgc cggcgaggcc gcgctggtgg agtgccatgg tcccgtgcgg
    10081 gccgagcacc tcctcgtcgg ccacgagctg caggacgagc ttgccgcgcc ggggcccgc
//
LOCUS       DATLFO010000362         2770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_138144, whole genome shotgun sequence.
ACCESSION   DATLFO010000362 DATLFO010000000
VERSION     DATLFO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138144"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..991
                     /locus_tag="VM841_14800"
     CDS             191..991
                     /locus_tag="VM841_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVL91494.1"
                     /translation="MRQVESRDGTMIAYWTSGEGPPLVVVHGTPADHTRWQPLLPYLE
                     PHLTVHAMDRRGRGASGDGPHYALEREFEDVAAVVDAVAEVSGSAVSVYGHSHGGLCA
                     FGAASLTSNIGKLVLYEGWPVPDPSVFALPSEVAGRIEALLADGDRDAVVETLFRHFE
                     LMSDEDLKAFKAAPSWAGRVAAAHTITRECRAEAGARLEAAMAAKIAIPTLLVTGENS
                     SDPSKAQVDAVVAALANARVTVIQDQEHVADVLAPELFAQTLIAFLEQ"
     gene            1201..2355
                     /locus_tag="VM841_14805"
     CDS             1201..2355
                     /locus_tag="VM841_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91495.1"
                     /translation="MSSLAEDFAAGILDKCDPPCLSLYQPTSRRHPENQQDPIRFGNL
                     VKSLEASLLQQVPKDEAGSLLEPLRALADDSSFWNHTLDGLVVLAAKGMFRVYKLQRP
                     VDELAVVADSFHTKPLVRILQSADRYQVLGLSRNEIKLYEGNRDMLDEIEPADDVPRT
                     MTDALGGELTEPHQTVASYGGAGSGQSPMHHGHGGKKAEVDIDAERFFRAVDRAVLEH
                     HSQPSGLPLILAALPEHHHMFHEISHNQFLMDDSIDVYPDALSSVDELRERVWQLVEP
                     QYLARLAKLVEEFGNARSKGLADDDLANVAKAVVGGRVATLLIEARREIPGRIDAETG
                     DIEFDDLAHPEVDDLLDDVGALAHKMGGHVVIVPAERMPTKTGVAAIYRY"
     gene            2582..>2770
                     /locus_tag="VM841_14810"
     CDS             2582..>2770
                     /locus_tag="VM841_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91496.1"
                     /translation="MSIDIKVAGPEQKARVVDVILLAFAQDPIARWAFPDPYDYVTIF
                     REFVPAFGGRAFDNESALH"
BASE COUNT          499 a          859 c          911 g          501 t
ORIGIN      
        1 ggatcgagaa gtgcattccc aagatcctcg ccgggaaagg tatgaacgat cggtaggtgt
       61 ctcgccgctt gcgtctggag cgactcgcgc gtgcaacgga tcgtccgcct gcggtgtccg
      121 gccgctcacg ccgtgtcctt atcccgatgg gagcccagga gttgacgtgc ttcaagagtg
      181 gaggtgaaga atgaggcagg tcgaatcccg tgacggcacc atgatcgcct actggacgag
      241 tggcgagggc ccgccgctcg tcgtcgttca cgggacgccc gccgaccaca cgcgctggca
      301 gccgctgctg ccctacttgg aaccacatct gaccgtgcat gcgatggatc gccggggtcg
      361 cggcgccagt ggtgacgggc cgcactacgc attggaacgc gagttcgaag acgtcgctgc
      421 agtggtcgat gccgtcgctg aggtttctgg gtctgcggtc agtgtgtatg gccactcgca
      481 tggcggcttg tgtgcattcg gtgctgcgtc cctgacgtcc aacatcggca agctcgtact
      541 ctacgaaggg tggcccgtcc cggacccttc tgtcttcgca ttgccgtcgg aggtggctgg
      601 ccgcatcgag gccctcttgg ccgatggtga tcgagatgcc gttgtcgaga cgctctttcg
      661 gcacttcgag ttgatgtccg acgaggacct gaaggccttc aaggccgctc cgtcgtgggc
      721 gggtcgtgtt gccgcggcgc atacgatcac gcgcgagtgt cgcgcagagg caggcgcacg
      781 gctcgaggcc gcgatggcgg caaagatcgc catcccaacg cttctcgtca ctggcgagaa
      841 tagctcggac ccttccaagg cacaggtcga tgccgtcgtc gcagcactgg cgaacgcccg
      901 cgtgacggtc atccaagacc aagagcacgt cgcagacgtc ctagccccag agctgttcgc
      961 tcagacactc atcgcgttcc ttgaacaata ggtgtggtgt gaaccgagat ggccggcagg
     1021 agggttgcag ggagtatcga gatggacggc cggctggcgg atcccggcgc tcttcgccat
     1081 ggggagcgag agcgccctgc atgcaattcg agcatgggga agctaccgag atgtgggtag
     1141 acatgacccc cgtccttggg gttgtcgagc acggagccga tcccattgga tggggacaac
     1201 gtgagctcac tcgcggaaga cttcgcagca gggattttgg acaagtgcga tcctccgtgc
     1261 ctttcgctct accagccaac gtctcgacgc catccggaga accagcagga tccgatccga
     1321 ttcgggaacc tggtgaagtc gctcgaggcg tccctgcttc aacaagtccc gaaggacgaa
     1381 gccgggtcgt tgctggaacc gttgcgggcc ttggcggacg acagctcgtt ctggaatcac
     1441 acccttgacg ggctggtcgt gctggccgcg aaaggcatgt tccgtgtcta caagctccag
     1501 cggccggtcg acgagctcgc cgtcgtagcg gacagctttc acaccaaacc gctcgtgcgc
     1561 atcctgcagt cggccgaccg gtatcaggtt ctgggcctga gccgaaacga gatcaagctc
     1621 tacgagggca accgtgacat gctggacgag atcgagccgg ccgacgatgt cccgcggacg
     1681 atgaccgacg cgctgggcgg agaactgacc gagccgcacc agaccgtcgc ctcgtatgga
     1741 ggcgccggaa gcgggcaatc gcccatgcac catggccacg gcgggaaaaa ggccgaggtg
     1801 gacatcgacg cggaacgctt tttccgcgcc gtggaccgtg cggttctgga gcaccactct
     1861 cagccatcgg gcctgccgtt aatccttgcg gcgctgcccg agcatcatca catgttccac
     1921 gaaatcagcc acaaccagtt cttgatggac gacagcatcg atgtctatcc ggatgccctg
     1981 tcgtccgtcg acgagcttcg cgagcgcgtc tggcagctcg tcgagccgca gtacctggcg
     2041 cgcctggcca aactcgtcga agagttcggc aacgcacggt ccaaagggct cgccgacgac
     2101 gacctggcga atgtcgccaa ggcggtcgta ggtgggcggg tcgcgacgct gctgatcgaa
     2161 gcccgccgcg agattcccgg ccgtatcgat gccgagaccg gcgacatcga gtttgacgat
     2221 ctggcacacc ctgaggttga cgatctgctc gacgacgtgg gggcgttggc ccacaagatg
     2281 ggcggacacg tcgtgatcgt ccccgccgaa cggatgccga ccaagacggg agtcgccgcg
     2341 atttaccgtt attgaggcga ggtgacgcca cggaacggct tgcacccgcg gcacttcggg
     2401 cggccggtgt cacactccgg ggcggagcag gccggttccg ggcgcgatcc cgggcatcga
     2461 cgcgtccgct ccggcccaaa gtgacggccg tcacactcgt aacgtcgcgt tcgcgatctg
     2521 ccatcccctg attcgacccc gtatattttc cttgggtggc atacgcagac gggaggtcat
     2581 catgagcatc gacatcaagg tggcggggcc ggaacaaaag gcgcgggtcg tcgacgtgat
     2641 cctgctggca ttcgcgcagg atccgatcgc ccgctgggcg tttcccgacc cgtacgacta
     2701 cgtgacgatc tttcgcgagt tcgttcccgc gttcggcggc cgtgcgttcg acaacgaatc
     2761 ggcgctgcac
//
LOCUS       DATLFO010000363        10739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18460, whole genome shotgun sequence.
ACCESSION   DATLFO010000363 DATLFO010000000
VERSION     DATLFO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18460"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VM841_14815"
     CDS             <1..547
                     /locus_tag="VM841_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91497.1"
                     /translation="NAGFIGPTARCVADGSASTDGTFSTSGTLTTPAAGYAPGSGYVD
                     GFAEMETTLPLAAPRQSVEIVATVLIERASSSQVGRLGYLAPRVASSTSITVFVFDGG
                     CECYVGGGQDITSTASDAEQQVVNRTVQVRVSLEDWFGSSVAGPVTVRVSASGRASTG
                     FAAVGSASWELSGRVLSVSVV"
     gene            595..1326
                     /locus_tag="VM841_14820"
     CDS             595..1326
                     /locus_tag="VM841_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVL91498.1"
                     /translation="MSGRLIVGTSGFAYADWKPRFYPEGLAQSKFLPHYATRLSGVEI
                     NYTFSRFPTAKLLDGWAAKVPPSFVLCLKTPKLITHQKRLRACETLFAGFAATAGALG
                     PRLGPLLVQLPPTMPADAALLRDFLAAAPPSVRLAFEFRDPSWDSGETREALAQTGAA
                     WVVAETGRGGAVMHRTADFVYLRLRRDAYDTDAIDGWITRIAALLAEDTDVFCFLRHD
                     ADGANAVAAERMIAALTEGGREQHA"
     gene            complement(1294..1932)
                     /locus_tag="VM841_14825"
     CDS             complement(1294..1932)
                     /locus_tag="VM841_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVL91499.1"
                     /translation="MYEAAIFDFGGVVTTPIADAFARFDEAVGVEPGTVLRLMREARE
                     EASSDFHLLETGEMSEADFYAALGVRVAAAAGRPVDWPADTQTIRRVLLGSLRRNEEM
                     LEAVAAIGRAHKVGMLTNNVREWSRWREYYPMELFRVVVDSCEVGMRKPDPRIYRLTC
                     EQLGVDPSRAVFVDDLQENVAGANAVGMRGVLFTTTGDTIAELRVLFPAAFG"
     gene            2004..2648
                     /locus_tag="VM841_14830"
     CDS             2004..2648
                     /locus_tag="VM841_14830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HVL91500.1"
                     /translation="MTIQIVDERIEAYMRGLLARYDEPVLLAMEAEAREKGFPIVERQ
                     VGVTLEVLARTLGAQRVFELGSGFGYSAYWFARGATEVFCTDGDPENAEKAETYLSKA
                     GIWDRIHFRVGDAVEGLGATDGEFDIVYCDIDKHGYPDAWRAARDRVRVGGLYICDNV
                     LWSGRVAEPRVDDDVRPGWSEAIRKHNELIANDPGYVSTIVPTRDGVMVATRIA"
     gene            complement(2653..3786)
                     /locus_tag="VM841_14835"
     CDS             complement(2653..3786)
                     /locus_tag="VM841_14835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVL91501.1"
                     /translation="MARRDAARLKIGFQDPTRPPYRLTQIVARLADRVRFDSYWFLDH
                     FMGVVPPGMWSAELTQAARLIRNPDEFFDPFVVVGAIGARTKRLRLGISVTDTVRLHP
                     AWIARAALTLQHTTKGRFILGIGAGERENLEPYGLSAERLAARCGESIAVIRLLWSSR
                     DPVDFEGEFFTLRGASLALRPYLQRPPPIWIAAMGPRMLAITGRYGDGWLPHWQSVER
                     YRSSLDRIRAAARDAGRDPAAITPGLVMTCIVDAKHEECHAALESPALKLAALLQDAV
                     AWRQAGGSHPLGEEFRGIVDFVPTRVSTDEVRAAMDTVPWEVLHALYPHGTPDQLADR
                     VRAYESAGLRHVIFENITAVGKPSKAISSFTALGKTARILRKG"
     gene            complement(3790..4623)
                     /locus_tag="VM841_14840"
     CDS             complement(3790..4623)
                     /locus_tag="VM841_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVL91502.1"
                     /translation="MRIDLHTHSSVSDGALPPAQVARAAKAAGLDCIALTDHDSMAGI
                     PEARATGDAIGLAVIAGCELSAVLEHKPVHMLGYFLDASHPRLSSELAAIRDFRLRRA
                     ERMVATLNELGVPITYDRVREIASGESVGRPHVAQAMVEAGVIATTNDAFTDEWIGNN
                     GRAYLEKTALSPREAVALIREAGGAAVIAHPIWVEGPVSAEELIRDLAGAGLAGVEVD
                     HPDHDGQVRERFRALADELDLVATGSSDYHGNSHGGALGGNTTSPGALERLRERAKAA
                     V"
     gene            complement(4679..6466)
                     /locus_tag="VM841_14845"
     CDS             complement(4679..6466)
                     /locus_tag="VM841_14845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91503.1"
                     /translation="MDVIVEVLRWTTTMVYLGAALVAFRMRRRSASAPATWLAAAFGS
                     LGLILVWGLFAPEPPYSPGWLWSAKVAVAILATFPYALMRFALSVGPAATRISRTVLA
                     SVGALVVWTFLLPEFYAQEAPKPAWYTPYVVTFVAVWFVGIIVAGRLLWTGGRGQAPV
                     VARRLRMMSIGAVLVASAVLGSLFAVGDARQPLEVRMVSSFLSGGSGLLFLLAFTAPR
                     WLRAFWRRSDEQATRGAAASCIRAQSVGELCHTLLPHVQGVVGGTGAVLVSTDGNVLG
                     SIGLTDADAKRIASSAGAGEHAASVLCIELEHGRLCVALNDRAPFAGDEETSLLHSFG
                     GFADAALERLALLERTDRMRAEFIAMLSHDMRAPLTAARGMIETLASEWDELDVTLRR
                     ELLARASSNGIKLGAMIEQMLEHSQLEAGRIETLVERVNLGRAVADVVEHLGTLLDGH
                     RVVTDVPPGLYAIADRQAVERILTNLVINAAKFAPAGTRITVAATRRGDTVTLDVSDE
                     GPGIPPEDRSRVFDSFYRGSQTHGSAGLGLGLAIVRRLAELQNGSVEIMAREYGSTFV
                     VVLPGGGERVSSVAGAAGAAGMTAERAAG"
     gene            complement(6685..7839)
                     /locus_tag="VM841_14850"
     CDS             complement(6685..7839)
                     /locus_tag="VM841_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL91504.1"
                     /translation="MDFDLTPEQEMIRKTARDFAEREIAPVAMEFDRNEEFPWPIVEK
                     MKPLGFLGAPLPVEYGGMGLDPVSYALVIEEIARADSSVRSIVSVNCSLVGLTIAKWG
                     SEEQKQHWLPLLSAGEKLGCFGLTEPGSGSDAASLKTSAKRHGDDWILNGQKMFITNG
                     TVAGVALTIATVDASLGAKGTVAFLVPCDTPGFSAREIKGKLGLRASSTAELYYDDVR
                     VPDSARIGEVGDGIKMALWTLDNGRVGLGASCVGLAQACLDAALKYAKEREQFGKPIA
                     GFQLVQEMLAEMLVEVEAARLLVWRAAAKKAKGERNTLEASIAKYAASEAAVKCANLA
                     IQVHGGYGYIDEFPVGKYLRDARVATLYEGTSQIQKLIMGRHLTGISAFT"
     gene            7984..9531
                     /locus_tag="VM841_14855"
     CDS             7984..9531
                     /locus_tag="VM841_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVL91505.1"
                     /translation="MTWEPELEELRRRRELAAQMGGPDKIGRQRAAGRLTVRERIDVL
                     LDPGSFREIGGLAGAADYDESGELKRFRAANFVMGRGRIDGRRIVVGGDDFTVRGGAA
                     DAAIYQKQVYAEQMAQTERIPIVRLVDGTGGGGSVKSLETMGRTYVPFNPGFEVAIAN
                     LATVPVVALALGPVAGLGAARVVMSHYSVMVRGIAQVFVAGPPVVAGLGERVDKETLG
                     GADVHARNGVVDDEAESEAAAFDRARRFLSYLPSSVYDLPPRIEGGDDPARADEALLS
                     AVPRERRTPYDMRAIIASVCDRGSVFEMARRFGTPAITALARLDGCPVAVLANDPRIY
                     AGGWNAAASQKVARFIDFAETFHLPLVHLVDQPGFVIGSAAERAATIRHGARALAAIY
                     QATVPWCSVIVRKAYGVAGAAHQNHARFNVRYAWPSGDWGSLPLEGGIEAAYRADLEA
                     ADDPAALLAEIRARMDAVRSPFRTAEAFLIEEIIDPRDTRRLLCEFAVSAAGRLSPGP
                     RGWGMRP"
     gene            9766..>10739
                     /locus_tag="VM841_14860"
     CDS             9766..>10739
                     /locus_tag="VM841_14860"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVL91506.1"
                     /translation="MTTNGIPSEIKELAERALGVATAAGATYVDARYLSEQSEQIDVQ
                     DDRVTGVGRGSTAGSRIEVPARLAVEIARSSRALMKGPVRMAGEPPHEASWSSPCAID
                     PFTVPLDQKIDLLLAATKAAKQVKELTIAEASIDLWRRTSWLATSDGTRIAQTITHTG
                     AGLRAIAIGGGEVQQRSYPNSFRGGFASAGWEYATAMDLPGNAPAYAEEAVALLSAPE
                     CPSGTTTLILTPDQLGLQVHESIGHPIELDRVLGMEAAYAGTSFLKPGDEGSLRYGSD
                     LINITADATLPGGLGTFGWDDEGVAAARTPLIEAGMLRAALSDRGSAAA"
BASE COUNT         1642 a         3788 c         3776 g         1533 t
ORIGIN      
        1 caacgcggga ttcatcgggc cgacggcccg ctgcgtcgcc gacggctccg cgtcgaccga
       61 tgggaccttc tcgacgagcg ggacgctgac gaccccggcc gcgggctacg cgcccggctc
      121 gggttacgta gacgggttcg cggagatgga gaccacgctg cctctagccg ctccacgcca
      181 gagcgtcgaa atcgttgcaa ccgtgctgat cgaacgagcg tcgtcgagcc aggtcggacg
      241 gctgggctat ctggctcccc gcgtcgcctc gagcacctcc atcacggtct tcgtgttcga
      301 cggaggctgc gagtgctacg tcggcggcgg ccaggacatc acgtcgacgg catcggacgc
      361 ggagcaacag gtcgtgaatc gcacggtcca ggtgcgggtc tcactcgagg actggttcgg
      421 gtcaagcgtt gccggcccgg tcacggttcg ggtgtccgcg agcggccgcg cctccaccgg
      481 gttcgcggcc gtcggatccg cttcgtggga gctgagcggc cgggttctgt ccgtctcggt
      541 ggtctgagcc ggacgttcgc atccgccgcc gccggaccgc taccatcgcg gctcgtgagc
      601 ggccgcctga tcgtggggac gtcggggttc gcgtacgccg actggaagcc gcgcttctac
      661 ccggaggggc tcgcccagtc gaagttcctg ccgcactacg cgacgcggct gtccggtgtg
      721 gagatcaact acaccttcag ccggttcccg accgccaagc tgctggacgg ctgggcggca
      781 aaggttcccc cctcgttcgt gctgtgcctg aagaccccga agctgatcac gcatcagaag
      841 aggctccgcg cgtgcgagac cctgttcgcc ggcttcgccg cgaccgccgg agcgctcggg
      901 ccgcggctgg gtccgctgct cgtccagctt ccgccgacga tgcccgccga cgccgcgctg
      961 ctgcgcgact tcctcgccgc cgctcccccg tcggtgcggc tcgccttcga gtttcgcgat
     1021 ccgtcgtggg attcgggcga gacgcgggag gcgctggcac agaccggggc ggcatgggtg
     1081 gtcgccgaga ccggccgggg cggcgccgtg atgcaccgga cggcggactt cgtctatctg
     1141 cggctgcgac gcgacgccta cgacaccgac gcgatcgacg gatggatcac gcgcatcgcg
     1201 gccctgctgg ccgaggatac cgacgtgttt tgcttcttgc ggcacgacgc ggacggcgcg
     1261 aacgccgtgg cggcggagcg gatgatcgcg gcgctaaccg aaggcggcag ggaacagcac
     1321 gcgtagctcg gcaatggtgt cgcccgtggt ggtgaacagc acgccgcgca ttcctaccgc
     1381 gtttgcgccg gcgacgtttt cctggaggtc gtccacgaac accgcgcgcg acggatcgac
     1441 gccgagctgc tcgcaggtga gccggtagat gcgcggatcc ggtttgcgca tgccgacctc
     1501 gcacgagtcg accacgaccc ggaacagctc catcgggtag tactcgcgcc agcggctcca
     1561 ctcgcgcacg ttgttggtga gcatgccgac cttgtgcgcg cggccgatcg cggcaaccgc
     1621 ctcgagcatc tcctcgttgc gacgcagcga tccgagcagg acgcggcgga tggtctgcgt
     1681 gtcggccggc cagtcgacgg gacggccggc ggcggcggcg accctgacgc cgagcgcggc
     1741 gtagaagtcg gcctcgctca tctcaccggt ctcgagcaga tgaaagtccg agctcgcctc
     1801 ctcacgcgcc tcgcgcatca ggcgaagcac ggtgccgggc tcgacgccga cggcctcgtc
     1861 gaagcgcgcg aacgcgtcgg cgatgggcgt ggtgaccaca ccgccgaagt cgaagatcgc
     1921 cgcctcgtac acgaccgcga gtatgccagc ccccgcgccg cgtcgtacga tgcggccgag
     1981 gacgccggac ccgggaggct gccgtgacga tccagatcgt ggacgagcgc atcgaggcct
     2041 acatgcgcgg gctgctcgca cgctacgacg agccggtgct gctggcgatg gaggccgagg
     2101 ccagggagaa aggctttccg atcgtcgagc gccaggtggg cgtgacgctc gaggtgctcg
     2161 cccgcacgct cggcgcgcag cgcgtgttcg agctgggcag cggcttcggc tacagcgcct
     2221 actggttcgc acgcggcgcg accgaggtgt tctgcaccga cggcgacccc gagaacgccg
     2281 agaaggccga gacgtacctg tcgaaggccg gcatctggga tcgcatccat ttccgcgtcg
     2341 gcgacgccgt cgaagggctc ggcgcgaccg acggtgagtt cgacatcgtc tactgcgaca
     2401 tcgacaagca cggctatccc gacgcgtggc gcgcggcccg cgaccgcgtc cgggtcggcg
     2461 ggctctacat ctgcgacaac gtgctgtggt ccggccgggt cgccgagccg cgggtggacg
     2521 acgacgtgcg gccgggatgg agcgaggcga tccgcaagca caacgagctg atcgcgaacg
     2581 atccgggcta cgtatcgacg atcgtgccga cacgcgacgg cgtgatggtc gccacgcgca
     2641 tcgcctagcc gcctatccct tgcgcaggat ccgcgcggtc ttgccgagcg cggtgaagct
     2701 cgagatcgcc ttggacggct tgccgaccgc ggtgatgttc tcgaagatca cgtgccgcag
     2761 cccggccgac tcgtaggcgc gcacccggtc cgcgagctga tccggcgttc catgcggata
     2821 cagcgcgtgc agcacctccc acgggaccgt atccatcgcc gcccgcacct cgtcggtgga
     2881 cacgcgcgtg ggtacgaagt cgacgatgcc gcgaaactcc tcgccgagcg gatgcgatcc
     2941 gccggcctgc cgccacgcaa ccgcgtcctg cagcagagcg gcgagcttga gcgcgggaga
     3001 ctcgagcgcg gcgtggcact cctcgtgttt ggcgtccacg atgcaggtca tcaccaggcc
     3061 gggcgtgatc gccgcgggat cgcgtccggc gtcgcgcgcc gcggcacgga tccggtcgag
     3121 cgacgaacgg tagcgctcca cggactgcca gtgcggaagc cagccgtccc cgtagcggcc
     3181 ggtgatcgca agcatgcgcg ggcccatcgc cgcgatccag atcggcggcg gccgctgcaa
     3241 gtacgggcga agcgccagcg acgcgccgcg cagcgtaaag aactctcctt cgaaatcgac
     3301 cgggtcgcgg ctgctccaca gcaggcggat caccgcgatc gactcgccgc agcgtgccgc
     3361 gagccgctcg gccgacaggc cgtagggctc gaggttctcg cgctcgcccg caccgatgcc
     3421 gaggatgaac cggcccttgg tggtgtgctg gagcgtgagc gccgcgcggg cgatccacgc
     3481 gggatgcagc cgcacggtgt cggtgaccga gatgcccagc cgcagccgct tggtgcgcgc
     3541 gccgatcgcg ccgacgacca cgaacggatc gaagaactcg tccgggttgc ggatcaggcg
     3601 cgcggcctgg gtgagttcgg ccgaccacat gcccggcggg accacgccca tgaagtgatc
     3661 caggaaccag taggagtcga aacgcacgcg gtcggcgagc cgggcgacga tctgggtcag
     3721 ccggtacggc ggccgggtgg gatcctggaa tccgatcttg agacgggccg catctcggcg
     3781 cgccatcggc tacacggccg ccttcgcccg ctcgcgaagc cgctcgagcg cgccgggcga
     3841 ggtcgtgttg ccgccgaggg ccccgccgtg cgagttgccg tgatagtcgc tcgatccggt
     3901 cgccaccaga tcgagctcgt cggcgagcgc gcggaaccgc tcgcgcactt ggccgtcgtg
     3961 gtcgggatga tcgacctcga ccccggcgag cccggcgccc gcgaggtcgc ggatgagctc
     4021 ctcggccgag accgggccct cgacccagat cggatgggcg atgaccgcgg cgccgccggc
     4081 ctcacggatc agcgcgaccg cctcgcgcgg agacagcgcg gtcttctcga ggtaggcgcg
     4141 gccgttgttg ccgatccact cgtcggtgaa ggcgtcgttg gtggtggcga tcacgccggc
     4201 ctcgaccatc gcctgcgcga cgtgcgggcg gccgacgctc tcgccggagg cgatctcgcg
     4261 cacccggtcg taggtgatgg gaaccccgag ctcgttgagc gtggcgacca tgcgctcggc
     4321 gcgccgcagc cggaagtcgc ggatcgccgc gagctcggac gacaggcgtg gatgcgacgc
     4381 gtcgaggaag tagccgagca tatggaccgg cttgtgctcg agcacggccg acagctcgca
     4441 tccggcgatc acggccaggc cgatcgcgtc gcccgtcgca cgcgcctcgg ggatgcccgc
     4501 catcgagtcg tgatcggtca gcgcgatgca gtccagaccg gcagctttcg cggcccgcgc
     4561 gacctgagcc ggcgggagcg ccccgtcgga gacgctcgag tgggtgtgca gatcgatccg
     4621 catgcgcggc aagtgtagcg gctcgctcgg cgacggccgc gtcgccggcg agccgccctt
     4681 aacctgcggc gcgctcggcg gtcatgccgg cggcgccggc ggcgccggcg acgctggaga
     4741 cgcgctcgcc tcccccggga aggaccacca cgaaggtgga gccgtactcc cgggccatga
     4801 tctcgacgct tccgttttgc agttcggcga gacgacgcac gatcgcaagc cccagcccca
     4861 ggccggcgct gccgtgcgtt tggctgccgc gatagaagct gtcgaagacg cggcttcggt
     4921 cctcgggcgg gatgccgggt ccctcatccg aaacatcgag ggtcaccgta tcgccgcgcc
     4981 gggtcgcggc cactgtgatg cgcgtcccgg cgggagcgaa tttggcggcg ttgatgacca
     5041 ggttggtcag gatgcgctcg acggcctggc gatcggcgat cgcgtagagg ccgggcggga
     5101 catcggtgac gacgcgatgt ccgtcgagca gcgtcccgag gtgctcgacg acgtcggcga
     5161 cggcgcggcc caggttgacc cgctcgacga gcgtctcgat cctgcccgcc tcgagctgcg
     5221 agtgctccag catctgctcg atcatcgcgc cgagcttgat accgttgctg gacgcgcggg
     5281 cgagcagctc gcgccggagc gtgacgtcga gctcgtccca ctcggaggcg agcgtctcga
     5341 tcatgccgcg ggcggcggtc agcggcgcgc gcatgtcgtg ggagagcatc gcgatgaact
     5401 cggcccgcat gcggtcggta cgctcgagca gcgcgagccg ctcgagcgcg gcgtcggcga
     5461 agccgccgaa ggagtgcagc agggaggttt cctcgtcccc ggcgaacggc gcccggtcgt
     5521 tgagcgccac gcagagacgg ccgtgctcga gctcgataca cagcaccgac gccgcgtgct
     5581 cgcccgcgcc ggcgctcgat gcgatgcgct tcgcgtccgc gtccgtgagc ccgatcgagc
     5641 ccagaacgtt cccgtcggtg gaaaccagca ccgcgccggt gccgcccacg acgccctgca
     5701 cgtgcggcag cagcgtgtgg cacagctcac cgacgctctg cgcgcggatg catgatgcgg
     5761 cggcaccccg tgtggcctgc tcgtccgagc ggcgccagaa agcgcgaagc caccgaggag
     5821 cggtgaacgc cagcaggaac agcaggccgc ttccgccgct caggaacgac gaaaccatgc
     5881 gcacctcgag cggctgccgc gcatcgccga cggcgaagag gctgccgagc acggcgctgg
     5941 cgacgagcac cgcgccgatc gacatcatgc gcaggcgccg ggcgacgaca ggcgcctggc
     6001 cgcggccgcc ggtccagagc aggcggccgg cgacgatgat gccgacgaac cacacggcca
     6061 cgaacgtcac gacgtacggc gtgtaccagg ccggcttggg cgcctcctgg gcgtagaact
     6121 cgggaagcaa gaaggtccag acgacgagcg cccccacgga ggcgagcacg gtccgcgaaa
     6181 tccgggtcgc cgccggtccc accgacagcg cgaaccgcat caacgcatac gggaaggtgg
     6241 ccaggatcgc gacggcgacc ttcgccgacc agagccatcc cggcgaatac ggaggctcgg
     6301 gggcgaacag gccccacacg aggatcagcc cgagcgagcc gaacgcggcc gcgagccagg
     6361 ttgccggcgc gctggcggag cggcgtcgca tccggaacgc gaccaaggcc gcgccgaggt
     6421 agaccatggt ggtcgtccag cgcaagactt cgacaatgac gtccatccac ccaccctccg
     6481 gcgacacggc tttcgctcta ttgtcgggat gacccaccgc aactgaatag cccgaacggg
     6541 ctaaataacc gggccgggtc agccgtgccg cggacaaacg ggcggaggca cccgggggcg
     6601 ttcaggtacg agtcagcagc aaccagacga tgaagccggt gacgacgaaa cccgccagaa
     6661 gctggagaag cgcacgcggc cggttcatgt gaaggcgctg atgccggtga ggtggcggcc
     6721 catgatcagc ttctggatct ggctcgtgcc ttcatagagt gtggccacgc gcgcgtcgcg
     6781 cagatacttg cccaccggga actcgtcgat gtagccgtaa ccgccgtgca cctggattgc
     6841 gaggttcgca cacttgacgg ccgcttcgct cgccgcgtac ttcgcgatcg aggcctcgag
     6901 cgtgttgcgc tcgcccttgg ctttcttcgc ggcggcccgc cacaccagca gccgcgcggc
     6961 ctcgacctcg accagcatct cggccagcat ctcctgcacg agctgaaagc cggcaatggg
     7021 cttgccgaac tgctcgcgct ccttcgcgta cttcagcgcc gcgtccaggc acgcctgcgc
     7081 gaggcccacg cacgacgccc cgagcccgac gcggccgttg tcgagcgtcc acagcgccat
     7141 cttgatgccg tcgccgacct cgccgatgcg cgccgagtcg ggcacgcgca catcgtcgta
     7201 gtacagctcc gcggtcgagc tcgcgcgaag accgagcttg cctttgatct cgcgcgcgga
     7261 aaagcccggc gtgtcgcacg gcaccaggaa cgcgaccgtg cccttcgcgc ccagagacgc
     7321 gtccacggtc gcaatcgtca gcgcgacacc ggcgaccgtg ccgttggtga tgaacatctt
     7381 ctgcccgttg aggatccagt cgtcgccgtg gcgcttggcg ctcgtcttga gcgaggccgc
     7441 gtccgatccc gagccgggct cggtcaaacc gaagcagccc agcttctcac ccgccgacag
     7501 caacggcagc caatgctgct tctgctcttc gctcccccac ttggcgatgg tcagcccgac
     7561 cagcgagcag ttcaccgaca cgatcgagcg caccgacgag tccgcgcgcg cgatctcctc
     7621 gatcaccagc gcatacgaaa ccggatccag acccatcccg ccgtactcga ccggcagcgg
     7681 cgcaccgagg aagccgagcg gcttcatctt ctcgacgatc ggccacggga attcttcgtt
     7741 gcggtcgaac tccatcgcga ccggcgcgat ctcgcgttcg gcgaagtcac gtgcggtctt
     7801 gcggatcatc tcctgctcgg gcgtcagatc gaaatccacg gtgaacgtcc tcctggcctg
     7861 ccggcggtcg gcgcagccac agcctaccgg ccggaaggaa accggcgggc ccggccacag
     7921 gcatcgagcg gtgaccggcc gattcttggc gggcgggcgg cgggctcccg tatcgtggcc
     7981 gccatgacgt gggagccgga actcgaagag cttcgccgcc gccgtgaact ggccgcccag
     8041 atgggcggac cggacaagat cgggcgtcag cgcgcggccg gccggctgac cgttcgcgag
     8101 cgcatcgacg tgctgctcga tcccggatcc ttccgcgaga tcggcgggct ggccggcgcg
     8161 gccgactacg acgagtccgg cgagctgaaa cgatttcgcg cggcgaactt cgtgatgggc
     8221 cgcggccgga tcgacgggcg gcgcatcgtg gtcggcggcg acgacttcac cgtgcggggc
     8281 ggcgcagccg atgcggcgat ctaccagaag caggtttacg ccgaacagat ggcacagacc
     8341 gaacgcatcc cgatcgtccg gctcgtcgac ggcaccggcg gcggcggctc ggtgaagtcg
     8401 ctcgagacga tgggccgaac ttacgtgccg ttcaacccgg gctttgaggt cgcgatcgcg
     8461 aacttggcga cggttccggt cgtggcgctc gcgctcggcc cggtcgccgg gctcggcgcc
     8521 gcgcgcgtcg tgatgagcca ctacagcgtg atggtacgcg gcatcgcgca ggtcttcgtc
     8581 gccggcccgc cggtggttgc ggggctgggc gagcgcgtgg acaaagagac gctcggcggc
     8641 gcggacgtgc acgcgcgcaa tggcgtcgtc gacgacgaag ccgaaagcga agccgccgcg
     8701 ttcgaccgcg cgcgccggtt cttgtcctat ctgccgtcaa gcgtctacga cctgccgccg
     8761 cgcatcgaag gcggagacga tcccgcgcgc gccgacgagg cgctgctttc ggcggtgccg
     8821 cgcgagcggc gtacgcccta cgacatgcgc gcgatcatcg cctcggtctg cgatcgcggc
     8881 tcggtgttcg agatggcccg ccgtttcggc acgcccgcga tcacggcgct cgcccgcctg
     8941 gacggctgcc cggtcgcggt gctcgccaac gatccgagga tctacgcggg cggatggaac
     9001 gcggccgcgt cgcagaaggt ggcgcggttc atcgacttcg ccgagacctt ccatctgcca
     9061 ctggtccatc tggtggatca gcccgggttc gtgatcggat cggccgccga acgggccgcc
     9121 acgatccggc acggcgcccg cgctctggcc gcgatctacc aggcgacggt cccgtggtgc
     9181 tcggtgatcg tgcgaaaggc ctacggcgtc gccggggccg cacaccagaa ccacgcgcgc
     9241 ttcaatgtgc gctacgcctg gccgagcggc gactggggat cgctgccgct ggaaggcggg
     9301 atcgaagccg cctaccgcgc cgacctggaa gccgccgacg acccggcggc gctgctggcc
     9361 gagatccgcg cgcgcatgga cgcggtccgc tcgccgtttc gcaccgccga ggcgttcctg
     9421 atcgaagaga tcatcgaccc gcgcgacacg cgccggctgc tgtgcgagtt cgccgtctcg
     9481 gccgccgggc gcctatcccc cggcccccga ggctggggca tgcgccccta agcccgctca
     9541 agtcgcccat cggccggtcc gataccggcg acagtcgctc gggagcgccc tcgtggcgat
     9601 cgcggccgct gccttcgggt tccgcgaccg cctgcccgcc gtgctcgaca ccttcacccc
     9661 cggccgtccc gcctagcgtc cttcattcgc cgcacgaccg acagcaagcg gccgcgtctt
     9721 cccgttcgtg acaccggcgg ggcgatgcga gtaccctgac ccgccgtgac gaccaacggc
     9781 attccttccg agatcaaaga gctcgccgag cgagccctcg gcgtggcgac cgccgccggc
     9841 gcgacctacg tggacgcccg ctacctgtcg gagcagtccg agcagatcga cgttcaggac
     9901 gatcgggtca ccggcgtggg ccgtggcagc acggcgggct ccaggatcga ggtgcccgcg
     9961 cgcctggcgg tcgagatcgc ccgctcctcg cgcgcgctga tgaaggggcc ggtgcgcatg
    10021 gccggcgagc cgccgcacga ggcgagttgg tcctcgccgt gcgcgatcga tcctttcacg
    10081 gtccctctcg accagaagat cgacctgctg ctcgccgcga ccaaggccgc gaaacaggtc
    10141 aaggagctca cgatcgccga ggcctcgatc gatctgtggc gccgcacctc gtggctggcg
    10201 accagcgacg ggacccgcat cgcgcagacc atcacgcaca ccggcgccgg cctgcgagcg
    10261 atcgcgatcg gcggcggcga ggtgcagcag cgctcctacc cgaactcctt ccgtggcggc
    10321 ttcgcctccg ccggctggga gtacgcgacg gcgatggatc tgcccggcaa cgcgccggcg
    10381 tatgccgagg aggcggtcgc gctgctatca gcgcccgaat gccccagcgg caccacgacc
    10441 ctgatcctga cgcccgacca gctcggcctg caggtgcacg aatcgatcgg ccatccgatc
    10501 gagctggacc gggtgctggg catggaagcg gcctatgcag gcacctcgtt cctcaagccc
    10561 ggcgacgaag ggtcgcttcg ctacggatcg gatctgatca acatcaccgc cgacgcgacg
    10621 ctgccgggcg gcctcggcac gttcggctgg gatgacgagg gggtcgctgc ggcgcgcacc
    10681 cccctgatcg aggccggcat gctgcgcgcc gccctctcgg accgcggctc ggcggccgc
//
LOCUS       DATLFO010000364        15831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_138207, whole genome shotgun sequence.
ACCESSION   DATLFO010000364 DATLFO010000000
VERSION     DATLFO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138207"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VM841_14865"
     CDS             <1..488
                     /locus_tag="VM841_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVL91507.1"
                     /translation="PEWELTPESQVGGIKSGPLPMGFNRAPVAKPGLLLAGDAAGIVN
                     PFNGEGIAYAMETGRIAAEVAHEAIALGDPRHLRVYPERLKEAYEGYYILGRAFVRLI
                     GHAKIMGALTKYGLPNDRLMRFAFRLLANLTEPRDGDASDKIINAMVRAAPAINTVVR
                     A"
     gene            612..935
                     /gene="ndhC"
                     /locus_tag="VM841_14870"
                     /pseudo
     CDS             612..935
                     /gene="ndhC"
                     /locus_tag="VM841_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            1067..1516
                     /locus_tag="VM841_14875"
                     /pseudo
     CDS             1067..1516
                     /locus_tag="VM841_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            1568..2125
                     /locus_tag="VM841_14880"
     CDS             1568..2125
                     /locus_tag="VM841_14880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVL91508.1"
                     /translation="MNETLTELCAGLRESFPEHVGEPVVEFGETSVRVARERVLDVCR
                     WLRDNGPFELLADLSGVDYLGIAPDEDRFMVAYHLYSFRRNERLRLRAFVPDGDERIE
                     SVATVWPSANWMEREVYDFFGIVFEGHPDLRRILMPDDWDGYPQRKDYPLGGTKVEFK
                     GVMVPPPNVRRQPSSTTTGYPGRIS"
     gene            2137..3399
                     /locus_tag="VM841_14885"
     CDS             2137..3399
                     /locus_tag="VM841_14885"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVL91509.1"
                     /translation="MYVGGQEWGEVVRPAGGDETLIVNMGPQHPSTHGVLRLVMELDG
                     ETVVACTPVIGYLHTGIEKNTEVRNWTQGVTFVTRADYLSPFFNELAYCLSVEKLLGI
                     EAPLRAQAIRVLMCELQRISSHLVWLATGGMELGALSMMLYGFREREKLLDIFENITG
                     LRMNHAYIRPGGLIQDLPESAPDLITAFLDEFPARIDDYEDLLSDNPLWLERNTGVGI
                     LEADDAIALGVTGPMLRAAGVPHDLRKVQPYSGYEKYDFEIAVDEGADCAARYRVRMR
                     EMRESAKIARQALEMLPSGPVMVEDPKIAWPAQMSLGPDGQGNSIEYIRKIMGESMEA
                     LIHHFKLVTEGFKVPAGQVYVPVESPRGELGYHVVSAGESKPWRVHMRDPSFVNLQAT
                     PHLVTGGLIADAIASIASLDPVMGGVDR"
     gene            3396..4028
                     /locus_tag="VM841_14890"
     CDS             3396..4028
                     /locus_tag="VM841_14890"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HVL91510.1"
                     /translation="MNEGTRILGVPRLPGPPRPPLSEHARAEAQRLMARYPEPRSALL
                     PMLYLVQADHGFVSREGVREIAQMLGLTPAEVTAVATFYTMYKREPMGRWLVSVCTQP
                     PCALRGANQIRDAIQQECGIGNGGTTEDGMISIEEVECLCICDGAPVVGINYENYENL
                     TPESAVELIRALRAGETPPKPARGDVPAPHDETHRELAGLNQKDREEAGA"
     gene            4025..5296
                     /gene="nuoF"
                     /locus_tag="VM841_14895"
     CDS             4025..5296
                     /gene="nuoF"
                     /locus_tag="VM841_14895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HVL91511.1"
                     /translation="MTAEMKILTARFDTEGADRYDGYTDSGGYEAMKKALGMDPADIV
                     ETIKTSGLRGRGGAGFPTGMKWGFINREDTPTYLVCNADEGEPGTFKDRELMERDPHS
                     VIEGMAIASYAIGCREMYIYVRGEFGYAARQMERAVAEAYDKGVLGRDIFGSGYDLDM
                     IVHRGAGAYICGEETALLESLEGYRGQPRLRPPFPAQKGLYQRPTVINNVETLACVPH
                     IIEKGSDWFRGIGTEKAPGTKMFSLSGDVERPGNYEVPFGTTARVLIEELAGGVSGGK
                     KLKAFTPGGASSTPIFGADKLDVSMDWESVQAAGSLLGTGAMMVWAEDACILRSMLRF
                     THFYAHESCGKCTPCREGTYWLEQMFVRLETGQGRDEDLAIMMDVQGNIGGRTFCALG
                     DFATAPVISSIKIFESEAREHIKKKGCPFER"
     gene            5286..7601
                     /locus_tag="VM841_14900"
     CDS             5286..7601
                     /locus_tag="VM841_14900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HVL91512.1"
                     /translation="MSDKQVTLTIDGREINVAEGTLVIRAAEQLGIYVPRFCDHPYLA
                     PLGACRQCMVEIEGQRKPLTSCTTMCTDGMVVKTQFTSEVAQDAQESVLEFLLINHPL
                     DCPQCDKGGECPLQDQTLAYGPGRSRFTDVKRRFVKPVPVNPLVYLDRERCVLCARCT
                     RFADEISGDPFIELFERGALEQVAIFEDDPYESNFSGNVIQICPVGALTSRNFRFRAR
                     PFDMTSSPSVCNLCASGCSLTIQERRGQIVRVLAGENPNVNDAWSCDLGRFGHEYVSS
                     PDRITEPMIKKDGEWVAVSWAEAIRVAAGRIESAKAEGAPVGVLAGQNLCDEDAYALS
                     RFARTVLQTNDVDHRLGHESAEHDAVIAAAVASESATNRSIDEALVILVAGIDLYEES
                     PIVFLRVKKAARAGAKVLEVNARRTPLSRLGGLSVLTKPGEEAGAVEQIAAAVEAAGD
                     RAVILCGDRLGPGAMSALWNLGVRTGASFGWVPRKGGAYGAVWAGLHPMLLPGGRRTG
                     QAADVERVWATTLPDGAGRPMPQILSSSQGVLLMAGVDPVSDGLDPAAAARGLDAHSF
                     VIALDMFMTPSTERADVILPVAAMPEREGTRSNWEGRAQAFLPAVDPAGLSSPDWEIL
                     GRIAAELHVAFPRTLDELRREMQALRRDPVARVAVDAPGAALPAGEGFALVTYRLLRD
                     SGTMMAGADELKTVVTPPFVELHPADATRLGITDGDTVTVTGPRGSGTAVARVGRSVS
                     EGAVFVPADQPGLRIADIADPSAGFVTVGRA"
     gene            7604..8788
                     /gene="nuoH"
                     /locus_tag="VM841_14905"
     CDS             7604..8788
                     /gene="nuoH"
                     /locus_tag="VM841_14905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVL91513.1"
                     /translation="MDWLDVAIVVAKTAVIFGFLLVQVMMTIWAERRVVAVMQQRIGP
                     NRVGPFGLLQSLADGVKLFFKEDIHPANADRPIYGLAPMVSLVPAFMAFAIVPLGPGI
                     TIGGRTIQLQLTDLNVGVLFFLAMGSLMVYGVMLAGWASGSAYPLLGAVRASAQTLSY
                     EMGMGLAVLAVVMWAGTMSTREIVDLQAGTYLYVIPRWFVGPQLPAFLLFMIAGFAEA
                     NRAPFDLPEAETELVGGYHTEYSGIKFAMFMMTEYVHIITTSAIAVTLFLGGWRGIHP
                     PFLEPLWGVLWFLGKTGFLIFLFMWVRATFPRIRYDRLMRFGWRVLLPAGLVWVMITA
                     VMVQLPREVDRTTLSAWTAVIFSVLFLIFAARVTRNTTRRRAALRAAGERMLGGGTRG
                     RR"
     gene            8802..9347
                     /gene="nuoI"
                     /locus_tag="VM841_14910"
     CDS             8802..9347
                     /gene="nuoI"
                     /locus_tag="VM841_14910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HVL91514.1"
                     /translation="MFTVAKHALKKPVTYQYPEVQKEYAPRYHGRHQLNRYEDGLEKC
                     IGCELCAWACPADAIFVQGADNTVELRYSPGERYASDYQINYLRCIFCGLCIEACPTR
                     ALTMTNEDFIADATRENLIYTKDRLMAPLPPKATKEPPFTDAEIAARGIDYYGGYAET
                     PKLTSADRAARTPEGDDGGAR"
     gene            9347..9964
                     /locus_tag="VM841_14915"
     CDS             9347..9964
                     /locus_tag="VM841_14915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVL91515.1"
                     /translation="MEQWIFWIVAPVSLGSAIAMLLARNTVHSAMLLVINFFTLAVFY
                     VVNDADFLAAVQIIVYAGAIMVLFLFVIMLIADKEEDLAERIKGQRTTAVLLGIGLAG
                     LIVFTIRTALSAADFRGLGAANTGGNVEAIGRVLFTEYLWPFEITSLLLIVAAIAAIV
                     IGRRPGDDPDDEEIEPGAAPAQPAAEPEAVSLAAASPPDHVEEAP"
     gene            9964..10263
                     /gene="nuoK"
                     /locus_tag="VM841_14920"
     CDS             9964..10263
                     /gene="nuoK"
                     /locus_tag="VM841_14920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVL91516.1"
                     /translation="MLPAQYLALAGLLFATGVVGVLARRNAIVVFMSIELMLNAVNLT
                     LVTFARMHGHLDGQVIAFFVMIVAAAEAVVGLALIVSIFRRKRTVSIDDVREMHG"
     gene            10260..12227
                     /gene="nuoL"
                     /locus_tag="VM841_14925"
     CDS             10260..12227
                     /gene="nuoL"
                     /locus_tag="VM841_14925"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVL91517.1"
                     /translation="MTSSGVFEAAWLIPGLPLLGAVLLLFLGRRMTKAAGGIATVMMA
                     GSFTIGLWTFFKLVSMPAAERLHTGTLYTFIKAGNFEVALDYRIDPLSVIMILVVTGV
                     GSLIHLYSIGYMRGDDRESRFFAYLNLFAFSMLVLVMANNMLIMYVGWELVGLCSYLL
                     IGFWTERPGAGLASKKAFITNRVGDVGLALGIMLIFVHFGTLDFSQVLSESGALGAGA
                     ATAIALLLFAGAAGKSAQIPLFVWLPDAMEGPTPVSALIHAATMVTAGVYMVGRMHPI
                     FAESAVAMNVVAWVGLATALLAALIALTQDDIKRVLAYSTVSQLGFMFMALGVGAYTA
                     AIFHLVTHAFFKALMFLGAGSVMHGLDDETDITKMGGLRTVMPWTAGTFVIGWLAIVG
                     FPGLSGFFSKDQILAGIYGSGNTALWIAALVATFLTGFYMSRLVFLTFFGRSRLAEGV
                     HPHESPAVMVWPLALLAIAAAGAGWLGATVHHGGFHRFVAPAVCGVHDEAHATEQTAV
                     PICTIDAEGEVHPVEQETMPSELVLGTVATAAGVAGLGLAGFMYLGGFPWERRRQSPT
                     LAWKASRNKFFVDEIYQFLFTGLGKAVAATAAFVIDRRVIDGTVSRIAHGVGALAVLG
                     RKLQTGLVRSYAVGVLGGVVVLGILLVARAR"
     gene            12235..13767
                     /locus_tag="VM841_14930"
     CDS             12235..13767
                     /locus_tag="VM841_14930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVL91518.1"
                     /translation="MLSTLIWLPLAGALALALFPKRSNTAVKAWAIMVGLASFGLALA
                     VLARFDAAAPGYQLVEDRWWIRSIGASYKIGVDGISLFLVLLTTFLVPISVLASWKED
                     RNPKLFCALLLALETGVLGVFLSLDLLLFTAFWDGMLIPMYFLIGYWGYEQRVYAAVK
                     FFIFTLLGGLLMFAGILVTYFQAKAALGVGTFDLEQIQTVSFSLGLQRWLFLAFFAAF
                     AIKIPLFPLHTWLPDAHTEAPTAGSVMLAAVLLKMGGYGFLRFAIPLFPQAAKEAVPW
                     VVALALTGIIYGAIVAIMQRDMKRLVAYSSVSHLGFVVLGIFVFTAQGMQGGTIQMIN
                     HGLSTGALFLLVGMLYERRHTRQISDYGGIAKVIPVYAGVFLFVAMSSLALPGLNGFV
                     GEFLILLGTFVESKPWAAIAAAGMVLGAVYLLWMYQRVFNGPITNPENSMIRDLSVRE
                     IVVLAPVLAGILLLGLFPKPVLDRMAPALDRVRARVTSPAVAPATTTVIEPAPATTGE
                     GR"
     gene            13768..15219
                     /gene="nuoN"
                     /locus_tag="VM841_14935"
     CDS             13768..15219
                     /gene="nuoN"
                     /locus_tag="VM841_14935"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HVL91519.1"
                     /translation="MPTPEIAWRAIAPILALAGGGVATLMLVAVSRRIHKGVLVGVCL
                     ASLGAAAWFNFDLNERQTVGMQGAVAADGVAIFGSMILIGAAALAILFAYEYLSARGI
                     HRPEFYPLLMFATAGMVLLASANDLLMVFLAVEVLSLALYVMTALARRDDKSQEGALK
                     YFLLGAFASAFLLYGIALAYGATATTNIARISETISAAPPRLSFLAFAFIAAGLGFKM
                     AAVPFHMWTPDVYEGAPTPVTAFMAAGTKTAALIALLRVFLVGFGPLEWDWQPLLWVI
                     AVASMALGSVVAIVQTDVKRMLAYSSIAHAGYLLVGVVAASRDGTSSTLFYMAVYAIA
                     TMGAFGVVLATAVRGRERLEMNAWQGIGRRSPAFAGAMTLFLLSLAGIPPTAGFMGKF
                     FIFQAAVESGEVALVVAGLLTSVVAAFFYLRLIVLMWMQEPDAAEHGVRPTPVLTAGL
                     AVGAAATIAFGVWPQELLDLARGASVFLGSVMP"
     gene            15216..>15831
                     /locus_tag="VM841_14940"
     CDS             15216..>15831
                     /locus_tag="VM841_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVL91520.1"
                     /translation="MTNADPLAGLELDIRSELDGVEAGLRAAVESAQPLVRDAAGHVL
                     ASGGKRFRPMLTLLCGRAGEYDSHAKAVASCGVAIELTHLATLYHDDVMDEAAVRRSR
                     PSANARFDNRVAILAGDWLFARASAIAADLGTYPSRVLADTIADVCEGQIMESEHTGS
                     PDQNVGRYLETIKRKTAALLATSCHLGAWLAGADAPIVEAVTAST"
BASE COUNT         2331 a         5319 c         5391 g         2790 t
ORIGIN      
        1 ctcccgaatg ggaactgacc cccgagagtc aggtcggggg catcaagagc gggccgttgc
       61 cgatggggtt caaccgcgcg ccggtcgcga agcccgggct gctcctggca ggcgacgccg
      121 ccggcatcgt caacccgttc aacggcgaag gcatcgccta cgcgatggag accggacgca
      181 tcgccgccga ggtcgcacat gaagcgatcg cgctcgggga cccgcgacac ctcagggtgt
      241 atcccgagcg gctcaaagag gcctacgagg ggtactacat cctcggacgg gcgttcgtcc
      301 gcttgatcgg tcacgccaag atcatgggcg cgctgaccaa gtacggactt ccgaacgacc
      361 gattgatgcg cttcgcgttt cgcctgctcg caaacctcac cgagccccgt gacggagacg
      421 cctcggacaa gatcatcaac gccatggtcc gggccgcacc ggcgatcaac acggtggtgc
      481 gcgcgtgagg cctacacatg gtgaactcgt gaagtcgttc gcggtttgca ccgggctcga
      541 aggggttcct atggtggccg cttcgagccg aaggagtggg cgcactggct gagtacctgc
      601 cgatcgtggt gatggcgggc ctcgcgacgt tgttcgcggg cgcgtccatc ttcgttgcgt
      661 ccaaagtcgg tccgcggcgt cccaacccgt cgaagtccgc agcctacgag agcggcatca
      721 cgccgatcca gacgccctcg tccgagcgat tctcggtgaa gttctatctg gtggcgatgc
      781 tcttcatcat cttcgacatc gagctggtgt tcttctatcc gtgggccgtg atcttccgcg
      841 agctcaagtt gttcgggctg atcgagatgg gcgtgttcgt ggcgctgctc ttcgtcgcgt
      901 acttctacat cattcggtcc ggcgcactgg actgggggga gaaaggccgg cggatcccgc
      961 ggcccgtccg ggggctggtc tcggaccgcg ccgcgctgca ctccccgggc gaagacggcc
     1021 aggacacgcc cgcggggccc gaaccccgga tcgaggcggt gcgctgatgg gcttggaaga
     1081 gaagcttccc tcggggatcc tgctcacctc ggtttcgaag ttcgtgaact gggcccgggc
     1141 cggctcgctg tggcccgcga cgttcgggct cgcgtgctgc gcgatcgaga tgatggccac
     1201 gggggcggcg caccacgatc tcgcgcggtt cggcatggag gtgttccgcg cctcgccccg
     1261 gcaggcagac ctgatgatcg tcgccggccg cgtgtcgcaa aagatggcgc ccgtccttcg
     1321 tcagatctac gaccagatgc ccgagccgaa atgggtgctc tcgatgggcg tgtgcgcgag
     1381 cgcgggcggc atgttcaaca actacgcgat cgtgcagggc gtggatcaga tcgtgcccgt
     1441 cgacgcctac gtcggcggct gccctccgcg tcccgagcag ctcatggacg cgatcttgct
     1501 gatccacgaa aagatcaagg gctccgacat caacgcgcgc atgcccgtgg cggacggcag
     1561 gtagacgatg aatgagacct tgacggagct gtgtgcggga cttcgtgaat cgtttcccga
     1621 gcacgtcggc gagccggtgg tggagttcgg cgagacctcg gtgcgggtcg cgcgcgagcg
     1681 cgtgctggac gtgtgccgct ggctgcgcga caacggtccg ttcgagctgc tcgccgacct
     1741 gtccggcgtg gactacctcg ggatcgcgcc cgacgaggac cggttcatgg tcgcgtatca
     1801 cctctactcg ttccggcgga acgagcggtt gcgcctgcga gccttcgttc ccgacggcga
     1861 cgagcggatc gagtcggtcg cgacggtgtg gccatcggcc aactggatgg agcgcgaggt
     1921 ctacgacttc ttcgggatcg tcttcgaggg ccatcccgac ttgcgccgga tcctgatgcc
     1981 ggacgactgg gacggctacc cccagcgcaa ggactatccg ctgggcggca ccaaggtcga
     2041 gttcaagggc gtgatggtcc cgccgccgaa cgtccggcgg cagccttcct ccaccacgac
     2101 cggctatccg gggaggatct cgtgagcgct cgcgagatct acgtcggcgg ccaggaatgg
     2161 ggcgaggtgg tccggccggc cggcggcgac gagacgctca tcgtcaacat gggcccgcag
     2221 catccatcca cgcacggggt gctcaggctc gtgatggagc tcgacggcga gacggtcgtg
     2281 gcgtgcacgc cggtcatcgg ctatctgcac accggcatcg agaagaacac cgaagtccga
     2341 aattggacgc agggcgtgac gttcgtgaca cgcgcggact acctgtcgcc gttcttcaac
     2401 gagctcgcct attgtctgtc ggtcgagaag ctgctcggga tcgaggcgcc gctgcgcgcg
     2461 caggcgatcc gggtcctgat gtgcgagctg cagcgaattt cgtcccacct ggtgtggctc
     2521 gcgaccggcg gcatggagct cggggcgctt tcgatgatgc tctacggctt tcgtgagcgc
     2581 gagaagctgc tcgatatctt cgagaacatc accgggctgc ggatgaacca cgcctacatc
     2641 cgcccgggcg ggctcatcca ggacctgccc gagtcggctc cggatctgat caccgcgttc
     2701 ctggacgagt tcccggcgcg catcgacgac tacgaagact tgctctcgga caacccgctc
     2761 tggctcgagc gaaacaccgg cgtgggaatt ctcgaagcgg acgacgcgat cgcgctcggg
     2821 gtcaccggcc cgatgctgcg ggccgccggc gtgccgcacg acctgcgcaa ggtccagcct
     2881 tacagcggct acgaaaagta cgacttcgag atcgcggtgg acgagggagc cgactgcgcc
     2941 gcccggtacc gggtgcggat gcgggagatg cgcgagtccg ccaagatcgc gcgccaggcg
     3001 ctcgagatgc ttccctcggg gccggtcatg gtcgaggacc cgaagatcgc ctggcccgcg
     3061 cagatgtcgc tcggccccga cgggcagggc aactcgatcg agtacatccg caagatcatg
     3121 ggcgaatcga tggaggcgct gatccaccac ttcaagctcg tcaccgaagg cttcaaggtc
     3181 cccgcgggcc aggtctacgt cccggtcgag tccccgcgcg gcgagctcgg ctatcacgtg
     3241 gtcagcgcgg gtgaatcaaa gccctggcgc gtccacatgc gcgacccgtc gttcgtcaac
     3301 cttcaggcca cgccgcatct ggtcacgggc ggcctcatcg cggatgcgat cgcgtcgatc
     3361 gcctcgctcg acccggtgat gggaggggtc gaccgatgaa cgaaggaacg cgcatcctgg
     3421 gcgtgccgcg tcttcccggc ccgccgcggc cgccgctgag cgaacacgcg cgcgccgagg
     3481 cgcagcggct gatggcgcgc tatccggagc cgcgaagcgc cttgctgccc atgctctatc
     3541 tggtgcaggc ggatcacggc ttcgtgtccc gggaaggggt tcgtgagatc gcgcagatgc
     3601 tcggcctgac gccggccgaa gtgaccgccg tcgcgacctt ctacacgatg tacaagcgtg
     3661 agccgatggg ccgctggctg gtcagcgtgt gcacacaacc gccgtgcgcg ctgcgcggcg
     3721 ccaatcagat ccgggacgcc atccagcagg agtgcgggat cggcaacggt ggcacgaccg
     3781 aggacggcat gatctcgatc gaagaggtcg agtgcctgtg catctgcgac ggcgctccgg
     3841 tggtcgggat caactacgag aactacgaga acctcacgcc cgagtcggcg gtggagctga
     3901 tccgcgcgtt gcgcgccggc gagacgccgc cgaagcccgc acgcggcgac gtgccggcgc
     3961 cgcacgacga aacgcaccgc gagcttgccg gcctcaacca gaaggatcgc gaggaggccg
     4021 gcgcatgacg gccgagatga agatcctcac cgcgcgcttc gacaccgaag gcgccgatcg
     4081 ctacgacggc tacacggatt ccggcggcta cgaagccatg aagaaggcgc tcgggatgga
     4141 cccggccgac atcgtcgaga cgatcaaaac ctcgggcctg cgcggccgcg gcggcgccgg
     4201 ctttcccacc ggcatgaagt ggggcttcat caaccgcgag gacacgccga cctatctggt
     4261 ctgcaacgcc gacgaggggg agcccggcac gttcaaggac cgcgagctga tggagcgcga
     4321 tccgcactcg gtcatcgaag gcatggcgat cgcgtcgtac gccatcggct gtcgcgagat
     4381 gtacatctac gtgcgtggcg agttcggcta cgccgcgcgg cagatggagc gcgcggtcgc
     4441 cgaggcctac gacaagggcg tgctcggccg cgacatcttc ggcagcggtt acgaccttga
     4501 catgatcgtg caccgcggtg cgggtgccta catctgcggc gaagagaccg cgctgctgga
     4561 gtcgctcgag ggctaccgcg gccagccgcg gcttcggccg ccgttcccgg cgcagaaggg
     4621 cctgtatcag cgtcccaccg tcatcaacaa cgtcgagacg ctcgcctgcg tgccccacat
     4681 catcgagaag ggatccgact ggttccgcgg catcggcacc gagaaggccc cgggcacgaa
     4741 gatgttcagc ctctccggcg acgtcgagcg gccgggcaac tacgaggtgc cgttcggcac
     4801 caccgcgcgc gtgctgatcg aagagctcgc cggcggtgtg agcggcggca agaagctcaa
     4861 ggcgttcacg cccggcggcg cgtcgtccac gccgatcttc ggcgccgaca agctcgacgt
     4921 gagcatggac tgggaatcag tgcaggccgc ggggtcgctg ctgggcaccg gggccatgat
     4981 ggtctgggcc gaggatgcct gcatcctgcg ctcgatgctg cgattcacgc acttctacgc
     5041 gcacgagtcc tgcggcaagt gcacgccctg ccgtgaaggc acctactggc tcgagcagat
     5101 gttcgtgcgc ctcgagaccg gccagggccg cgacgaggac ctggcgatca tgatggacgt
     5161 gcagggcaac atcggcggcc gcacgttctg cgcgctcggc gacttcgcaa ccgcgcccgt
     5221 gatcagctcg atcaaaatct tcgagtccga ggcccgcgag cacatcaaga agaaggggtg
     5281 tccgtttgag cgataagcag gtcaccctca ccatcgacgg tcgcgagatc aacgtcgcgg
     5341 aaggcacgct cgtcattcgc gcggccgagc aactcggcat ctacgtgccg cgcttctgcg
     5401 accacccgta tctcgcgccg ctgggtgctt gccgccagtg catggtcgag atcgaaggcc
     5461 agcgcaagcc gctcacgtcg tgtaccacca tgtgcaccga cggcatggtc gtcaagaccc
     5521 agttcacctc cgaggtcgcg caggacgcgc aggagagcgt gctggagttc ctgctgatca
     5581 accatccgct cgactgtccg cagtgcgaca agggtggcga gtgtccgttg caggaccaga
     5641 cgctcgccta cgggccgggc cgctcgcgct tcaccgacgt caagcgccgc ttcgtcaagc
     5701 cggtgccggt gaacccgctc gtgtacctgg accgcgaacg ctgcgtgctg tgcgcgcgct
     5761 gcacccgctt cgccgacgag atcagcggcg atccgttcat cgagctgttc gaacgcggcg
     5821 cgctcgaaca ggtcgcgatc ttcgaagacg atccctacga gtccaacttc agcgggaacg
     5881 tgattcagat ctgtccggtc ggcgcgctca cctcgcgcaa cttccgcttc cgggcgcggc
     5941 cgttcgacat gacctcgtcc ccgagcgtct gcaacctgtg cgcgtcggga tgctcgctca
     6001 cgatccagga acggcgcggc cagatcgtgc gcgtgctcgc cggggagaac ccgaacgtca
     6061 acgacgcgtg gtcctgcgat ctgggccgct tcgggcacga gtatgtctcg tcgccggatc
     6121 gcataacgga gccgatgatt aaaaaggacg gcgagtgggt cgccgtttcg tgggcggagg
     6181 ccatccgcgt cgcggcgggc cgtatcgagt ccgccaaggc cgagggcgcg ccggtcggcg
     6241 tgctcgcggg ccagaacctg tgtgacgagg atgcgtacgc gctttcgcgc ttcgcgcgca
     6301 cggtgctgca gaccaacgac gtcgatcacc gcctcggcca cgaaagcgcc gagcacgacg
     6361 cggtgatcgc cgcggccgtg gcgagcgagt cggcgacgaa ccgatcgatc gacgaggcgc
     6421 tcgtgatcct ggtcgcgggc attgatctct acgaggagtc cccgatcgtc ttcctgcgcg
     6481 tgaagaaggc cgcgcgcgcc ggcgcgaagg tgctcgaggt caacgcacgc cgcacgccgc
     6541 tctcccggct cggcggcctg tccgtcctga cgaagcccgg cgaagaggcc ggcgcggtcg
     6601 agcagatcgc cgccgccgtc gaagccgccg gcgaccgtgc cgtgatcctg tgcggcgacc
     6661 ggctggggcc gggcgccatg agcgcgctgt ggaatctcgg cgtgcgcacc ggcgcgtcct
     6721 tcggctgggt tccgcgcaag ggcggcgcct acggcgccgt gtgggcgggt ctgcatccga
     6781 tgctgcttcc cggcggccgc cgaaccggcc aggccgccga cgtcgagcgg gtctgggcca
     6841 ccacgctgcc ggacggggcc gggcggccca tgccgcagat cttgtcctcc tcgcagggcg
     6901 tgctgctgat ggccggagtg gatccggtct cggacggcct ggatccggcg gccgccgccc
     6961 gcggcctcga cgcgcattcg ttcgtcatcg cgctcgacat gtttatgacg ccctcgaccg
     7021 agcgcgccga cgtgatcctt cccgtcgcgg cgatgcccga gcgcgagggc acgcgcagca
     7081 actgggaggg tcgcgcgcaa gcattccttc cggcggtgga tcccgcaggc ctttcctcgc
     7141 ccgattggga gatcctcggc cgcatcgccg ccgagctcca cgtcgcgttc ccgcggactc
     7201 tggacgagct gcgccgcgag atgcaggcct tgcggcgcga tcccgtcgcg cgcgtcgcgg
     7261 tcgacgcgcc cggtgcggca ctgccggccg gcgagggctt tgccctcgtc acctaccggc
     7321 tgctgcgcga cagcggcacc atgatggcgg gggccgacga gctcaagacc gtcgtaacgc
     7381 cgccgttcgt cgagcttcat cccgccgacg cgacccggct gggcatcacc gacggcgaca
     7441 cggtcaccgt taccgggccg cgagggagcg gcacggccgt tgcgcgtgtc gggcgatccg
     7501 tctccgaagg tgcggtgttc gtgcccgccg atcagcccgg cctgcggatc gccgacatcg
     7561 ccgatccgtc cgcgggcttc gtcacggtgg ggagggcgta gggatggact ggctcgacgt
     7621 cgccattgtc gtggccaaga cggccgtgat cttcggcttc ctgctcgtgc aggtcatgat
     7681 gacgatctgg gccgagcgtc gcgttgtggc ggttatgcag cagcgcatcg gcccgaaccg
     7741 ggtcggcccg ttcgggctgc tccagtcgct ggccgacggc gtgaagctgt tcttcaaaga
     7801 ggacatccat ccggccaacg cggaccgtcc gatctatggg ctcgcgccga tggtgtcgct
     7861 ggtcccggcg ttcatggcgt tcgcgatcgt cccgctcggc ccgggaatca cgattggagg
     7921 ccgcacgatc cagctgcagc tcaccgacct caacgtcggg gtcttgttct tcctcgcgat
     7981 gggttcgctg atggtctacg gcgtcatgct cgccggctgg gcgtcgggaa gcgcgtatcc
     8041 gctcctcggt gccgttcgag cgagcgcgca gaccctgtcc tacgagatgg gcatgggcct
     8101 ggccgtactc gcggtggtga tgtgggcggg aacgatgtcc acgcgggaga tcgtggacct
     8161 gcaagcagga acctatctgt acgtgatccc acgatggttc gtcggcccgc agcttccggc
     8221 gttcctgctg ttcatgattg ccggcttcgc cgaggccaac cgcgctccgt tcgacctgcc
     8281 ggaagccgag accgagctcg tcggcggcta tcacaccgag tactccggca ttaagttcgc
     8341 gatgttcatg atgaccgagt acgtccacat catcacgacg tcggcgatcg cggtcacgct
     8401 gttcctgggt ggatggcgcg gcattcaccc gccgttcctc gagccgctgt ggggtgtgct
     8461 gtggttcctc ggaaagacgg ggttcttgat ctttttgttc atgtgggtgc gcgcgacgtt
     8521 tccccgcatc cgctacgacc ggctgatgcg gttcgggtgg cgtgtgcttc tgccggccgg
     8581 tctggtgtgg gtgatgatca ccgcggtgat ggtccagctc ccccgcgagg tcgaccggac
     8641 gacgctgtcg gcgtggacgg ccgtgatctt cagcgtgctg ttcctgatct tcgccgcgcg
     8701 cgtcacgcga aacacgacca ggcgccgcgc cgcgctgcgc gcggccggcg agcgaatgct
     8761 gggaggtggc actcgtggcc gtcgctgagt tcctccgcag tatgttcacc gtcgccaagc
     8821 atgcgctcaa gaagcccgtc acctaccagt accccgaggt ccagaaggag tacgcgcccc
     8881 ggtatcacgg ccgccatcag ctcaaccgct acgaagacgg cctcgagaag tgcatcggct
     8941 gtgagctgtg tgcctgggcg tgcccggccg acgcgatctt cgtgcaggga gccgataaca
     9001 ccgtggagct gcgttactcc cccggggagc ggtacgcgtc cgactaccag atcaactacc
     9061 tgcgttgcat cttttgcggc ctgtgcatcg aggcgtgccc cacgcgcgcc ctgacgatga
     9121 ccaacgagga cttcatcgcc gacgcgaccc gcgagaacct catctacacc aaggatcgtc
     9181 tcatggcccc gcttcccccc aaagccacca aggagccgcc gttcaccgac gccgagatcg
     9241 ccgctcgcgg catcgactac tacggcgggt acgccgagac gcccaagctc accagcgccg
     9301 accgagccgc gcgtacgccc gagggtgacg acgggggggc ccgctgatgg agcagtggat
     9361 cttctggatc gtcgcgccgg tctcactcgg cagcgccatc gccatgctgc tggcccggaa
     9421 cacggtgcac tcggccatgc tgctcgtgat caacttcttc actctcgccg tcttctacgt
     9481 ggtcaacgac gccgacttcc tggccgcggt gcagatcatc gtctacgccg gcgcgatcat
     9541 ggtcctgttc ctgttcgtga tcatgctcat cgccgacaag gaagaggacc tcgccgagcg
     9601 aataaagggc cagcgaacga cggccgttct gctcggcatc ggactcgccg gcctgatcgt
     9661 gttcaccatt cgcacggcgc tcagcgccgc cgacttccgc gggctggggg cggccaacac
     9721 cggcggcaac gtcgaggcca tcggccgggt gctgttcacc gagtacctct ggccgttcga
     9781 gatcacctcg ctgctcctga tcgtcgcggc gatcgccgcg atcgtcatcg ggcgccgccc
     9841 cggggacgac cccgatgacg aggagatcga gccgggggca gcgccggcgc aaccggccgc
     9901 cgagccggag gccgtttccc tcgcggccgc ctctcccccg gaccatgtgg aggaggcgcc
     9961 gtgatcctgc ccgcgcaata cctcgcgctc gccggcctgc tgttcgccac cggcgtggtc
    10021 ggtgtgctcg cgcggcggaa cgccatcgtg gtgttcatgt ccatcgagct catgctcaac
    10081 gcggtgaacc tgaccttggt cacgttcgct cggatgcacg gccacctcga cggccaggtg
    10141 atcgccttct tcgtgatgat cgtcgcggcc gcggaggccg tggtcggtct cgcgctgatc
    10201 gtgtcgatct tccggcgcaa gcgcaccgtg tcgatcgacg acgtgcggga gatgcacgga
    10261 tgacctcgtc cggcgttttc gaggccgcct ggctcatccc gggccttccg ctgctcggtg
    10321 cggtcctgct gctgttcctg gggcggcgca tgaccaaggc cgcgggcggc atcgcgaccg
    10381 tcatgatggc cggctcgttc accatcgggc tctggacgtt cttcaagctc gtgtcgatgc
    10441 cggccgccga acggctgcac accggcaccc tctacacgtt catcaaggcc ggaaacttcg
    10501 aggtcgcgct cgactaccgg atcgacccgc tgagcgtgat catgatcctg gtcgtgaccg
    10561 gcgtgggatc tctgatccac ctgtattcga tcggttacat gcgcggcgac gatcgagagt
    10621 cgcgattctt cgcctatctc aacctgttcg ccttctcgat gctcgttctg gtgatggcga
    10681 acaacatgct gatcatgtac gtcggctggg agctggtcgg cctgtgctcc tacttgctca
    10741 tcgggttttg gaccgaacgg ccgggcgcgg gcctcgcgtc caagaaggcg ttcatcacca
    10801 accgcgtggg cgacgtgggc ctggccttgg gcatcatgct gatcttcgtc cacttcggca
    10861 ccttggactt ctcgcaggtt ttgagcgagt ccggcgcgct cggagccggc gcggccaccg
    10921 caatcgctct gctgctgttc gccggcgccg ccggcaagag cgcgcagatc ccgctcttcg
    10981 tttggctgcc cgacgcgatg gagggcccga cgccggtctc ggcgctcatc cacgcggcga
    11041 ccatggtcac cgcgggcgtc tacatggtcg gccggatgca tcccatcttc gccgagtcgg
    11101 ccgtggcgat gaacgtggtc gcctgggtcg ggctcgcgac cgcgttgctc gccgccctga
    11161 tcgccctgac ccaggacgac atcaagcgcg tgctggccta ttcgacggtg agccagctcg
    11221 ggttcatgtt catggccctg ggcgtcggcg cctacaccgc cgcgatcttc cacctggtca
    11281 cgcacgcgtt cttcaaggcg ctgatgttcc tcggggcggg cagcgtgatg cacggcctcg
    11341 acgacgagac cgacatcacc aagatgggcg gcctccgcac ggtgatgccc tggacggccg
    11401 ggacgttcgt gatcggctgg ctcgccatcg tggggttccc cgggctcagc gggtttttct
    11461 cgaaggatca gattctcgcg ggcatctacg gcagcgggaa caccgcgctg tggatcgccg
    11521 cgttggtggc caccttcctg accggcttct acatgtcgcg gttggtgttc ctgacgttct
    11581 tcggccgcag ccggcttgcc gagggcgttc atccgcacga gtcacccgcg gtcatggtct
    11641 ggccgctggc gctgctggcc atcgcggcgg ccggtgcggg ctggctcggc gcgaccgtgc
    11701 atcacggcgg cttccaccgt ttcgtggctc cggcggtgtg cggcgtgcac gacgaggcac
    11761 acgcaaccga gcagaccgcc gtccccatct gcacgatcga cgccgagggc gaggtgcacc
    11821 cggtcgagca agagaccatg ccgagcgagc tggtgctcgg caccgttgcc accgcggccg
    11881 gcgtcgcggg gctcgggctg gcggggttca tgtatctcgg cggcttcccg tgggagcggc
    11941 gccgccagag cccgacgctg gcgtggaagg cgagccgcaa caagttcttc gtcgacgaga
    12001 tctaccagtt cctgttcacc ggcctcggca aggcggtcgc cgccacggcg gcattcgtta
    12061 tcgaccggcg ggtgatcgac ggaacggtca gccgcatcgc gcacggcgtg ggcgcgctcg
    12121 cggtcctggg ccgcaagctt cagaccggcc tggtgcgctc gtacgccgtc ggcgtgctcg
    12181 gcggcgtcgt tgtcctggga atcctgctcg tggcgagggc gaggtgatgc ggcgatgctg
    12241 agcacgctca tctggctgcc gctcgccggc gcgctcgcgc tcgcgctgtt tcccaagcgc
    12301 agcaacaccg ccgtcaaggc gtgggcgatc atggtcgggc tggcctcgtt cgggctcgcg
    12361 ctcgcggtgc tggcgcgctt cgacgccgcg gcgcccggct atcagttggt cgaggaccgc
    12421 tggtggatcc ggtcgatcgg cgcctcgtac aagatcggcg tggacggcat ctcgctgttc
    12481 ctggtcttgc tgaccacgtt cctggtcccc atcagcgtgc tcgcgtcctg gaaggaagac
    12541 cgcaacccga agctgttctg cgcgctgctg ctcgcgctcg agaccggcgt gctcggcgtg
    12601 ttcctatctc tggacctgct gctgttcacc gccttctggg acggcatgct catcccgatg
    12661 tacttcctga tcggctactg gggctacgag cagcgtgtct atgccgcggt gaagttcttc
    12721 atcttcacgc tgctgggcgg gctgctcatg ttcgccggca ttctcgtcac ctacttccag
    12781 gccaaggccg cgctcggtgt gggcaccttc gacctcgagc agatccagac ggtgtcgttc
    12841 tcgctgggcc tgcagcggtg gctgttcctc gcgttcttcg ccgccttcgc catcaagatc
    12901 ccgctgttcc cgctgcacac ctggctgccc gacgcgcaca ccgaagcgcc gaccgccggc
    12961 tcggtcatgc tggccgcggt gcttctcaag atgggcggct acggcttctt gcgcttcgcg
    13021 atcccgctat tcccgcaggc cgccaaggaa gcggttccgt gggtcgtcgc gctggcgctg
    13081 accggcatca tctacggagc gatcgtggcg atcatgcagc gggacatgaa gcggctcgtt
    13141 gcgtattcga gcgtaagtca tctcggcttc gtggtgctcg gcatcttcgt tttcacggcg
    13201 caaggcatgc agggcggcac catccagatg atcaaccacg gcctgtccac cggcgcgctg
    13261 ttcctgctcg tgggcatgct ctacgaacgg cggcatacgc gtcagatctc cgactacggc
    13321 gggatcgcca aggtcatccc ggtctacgcg ggggtctttt tgttcgtcgc gatgtcctcg
    13381 ctcgcgctgc ccggcctcaa cggcttcgta ggcgagttcc tgatcctgct cggcaccttc
    13441 gtcgagtcca agccgtgggc cgcgatcgcg gccgcgggta tggtgctcgg agcggtgtat
    13501 ctgctgtgga tgtatcagcg ggtgttcaac ggcccgatca ccaatccgga gaactcgatg
    13561 atccgtgacc tgtcggtgcg cgagatcgtg gtgctcgctc cggtccttgc gggcatcctg
    13621 ctgctgggcc tgttccccaa gcccgtgctc gaccggatgg ccccggcgct ggaccgggtg
    13681 cgcgcgcggg tgacctcgcc cgcggttgcg cccgccacca cgacggtgat cgagcccgcg
    13741 cccgccacga cgggggaggg acgctagatg ccgacgcccg agatcgcctg gcgggcgatc
    13801 gctcccattc tcgctctggc gggcggcggc gtggctacgt tgatgctcgt ggccgtgagc
    13861 cgccggatcc acaaaggcgt gctcgtgggc gtctgcctgg cgtcgctcgg cgccgcggca
    13921 tggttcaact tcgacctcaa cgagcgccag accgtgggca tgcagggcgc ggtcgccgca
    13981 gacggcgtcg cgatcttcgg ctcgatgatc ctgatcggcg cggcggcgct cgcgatcctc
    14041 ttcgcctacg agtacctgtc ggcccgggga atccaccggc cggagttcta tccgctgctg
    14101 atgttcgcga cggcgggcat ggtcctgctg gcgtccgcga acgatctgct gatggtcttt
    14161 cttgcggtgg aggtgctctc gctcgccctg tacgtcatga cggcgcttgc gcgtcgcgac
    14221 gacaagagcc aagagggggc gttgaagtat ttcctgctgg gcgcgttcgc gagcgcgttc
    14281 ctgctgtacg gcatcgcgct cgcgtacggc gcgacggcca ccaccaacat cgcgcgcatc
    14341 agtgagacga tctcggccgc gccgccccgc ttgtcgttcc tcgcgttcgc cttcatcgcc
    14401 gccggcctcg ggttcaagat ggccgccgtt cccttccata tgtggacacc cgacgtgtac
    14461 gagggcgcgc ccacgccggt gacggccttc atggccgccg gcaccaagac tgccgcgctc
    14521 atcgccctgc ttcgggtgtt cctggtggga ttcggtccgc tcgaatggga ctggcagccg
    14581 ctgctgtggg tcatcgcggt cgcgtcgatg gcgctcggat ccgtcgttgc aatcgtgcag
    14641 accgacgtca agcgcatgct cgcgtattcg tcgatcgcgc acgccggcta cctgctggtc
    14701 ggagtggtcg cggcgagccg ggacggcacc tcgagcacgc tgttctacat ggccgtctac
    14761 gcgatcgcga ccatgggcgc cttcggcgtc gttctcgcca ccgcggtgcg gggccgggag
    14821 cggctcgaga tgaacgcatg gcaaggcatc ggccgccgct cgcctgcctt cgccggggcc
    14881 atgacgctgt tcttgctctc gctcgccggg atcccgccga cggcgggctt catgggcaag
    14941 ttcttcatct ttcaagcggc cgtcgagtcc ggcgaggtcg cgctggtcgt cgccggcctg
    15001 ctcaccagcg tggtcgcggc gttcttctat ctccggctga tcgtgctgat gtggatgcag
    15061 gagccggacg ccgccgagca cggcgtcagg cccacgccgg tgctgacggc cgggctcgcc
    15121 gtcggggccg ccgcgacgat cgccttcggc gtctggccac aagagctgct cgacctcgcg
    15181 cgcggcgcgt cggtcttcct cggctcggtg atgccgtgac caacgccgat ccgctcgccg
    15241 ggctcgagct cgacatccgc tccgagctcg acggagtcga ggccgggctg cgcgccgcgg
    15301 tcgagtcggc gcagccgctg gtgcgcgacg cggccgggca cgtgctggcg agcggtggca
    15361 agcgcttccg gccgatgctc acgctgctgt gcgggcgtgc cggcgaatac gactcgcacg
    15421 cgaaggcggt cgcgtcctgt ggcgtggcga tcgagctgac ccacctggcc acgctctacc
    15481 acgacgacgt gatggacgag gccgcggtgc gccgcagccg gcccagcgcg aacgcgcgct
    15541 tcgacaaccg ggtcgcgatc ctcgcggggg attggctgtt tgcccgcgcg tcggccatcg
    15601 cagcggatct gggaacatac ccgtcgcgcg tgctcgccga taccatcgcc gacgtgtgcg
    15661 agggtcagat catggaatcc gagcacaccg gctcgcccga tcagaacgtc ggccggtacc
    15721 tcgagacgat caagcgaaag accgcggcgc ttctggcaac gtcgtgccat ttgggcgcat
    15781 ggctggccgg cgcggacgcg cccatcgtcg aggcggtgac cgcctcgacg a
//
LOCUS       DATLFO010000365         5968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_99054, whole genome shotgun sequence.
ACCESSION   DATLFO010000365 DATLFO010000000
VERSION     DATLFO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99054"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1541
                     /locus_tag="VM841_14945"
     CDS             168..1541
                     /locus_tag="VM841_14945"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HVL91521.1"
                     /translation="MIHLPQEVAELAHRFVKQGFELYLVGGSVRDAVLGRLHDDLDFA
                     TSAKPNEILEVVRGWADATWLTGIEFGTVGVARRGHRLEITTFRGEKYREDSRKPLIE
                     TVSTIEADLARRDFTINAMALRLPERALVDPHGGIGDLAAKVLRTPLAPEVSFSDDPL
                     RMLRAARFAAVLDCVPVPELVEAMTAQRARLKIVSAERIRDELSKLLAAPKPSIGLDL
                     ATSAGLCDLFLPELPALKLEQDPVHRHKDVYRHTLAVVDKLAAGDPEQQPDVLLRMAG
                     LLHDIAKPATRRFDKGKVTFHHHEVVGAQMTAKRLRALRYPGEFVDDCAALVEMHLRF
                     HTFRMGWSDSAVRRYVRDAGGLLDRLNRLVRADCTTRNALKAKRLSDAMDQLESRIAE
                     LASQEDLKRIRPPLNGHEVMQYLAVPPGPAVGRALDHLLEIRLDRGEYSKDEAYAMLD
                     AWKAEHL"
     gene            1641..1988
                     /locus_tag="VM841_14950"
     CDS             1641..1988
                     /locus_tag="VM841_14950"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HVL91522.1"
                     /translation="MSEPDGARTPQTPTTAGQRDAEQDQQHLRVIAAERRRRAIKLGA
                     LATLVVLFAIFVVQNSQPAPVDFIFVQSSPRLIWIMLVCAVIGGVIGYILGRPGKTLR
                     FHRKDDKQQKPAR"
     gene            complement(1985..2290)
                     /locus_tag="VM841_14955"
     CDS             complement(1985..2290)
                     /locus_tag="VM841_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91523.1"
                     /translation="MSGSPSRYAFEEHLDGSVRIRATLEGEQFDVLFTPGVDFFGPMG
                     VGGSIGCGPDNPGIGPAVSGPPYEATGRGFGVEIGEVYRATSALQPTLCPLDPIRRP"
     gene            complement(2458..2850)
                     /gene="sodN"
                     /locus_tag="VM841_14960"
     CDS             complement(2458..2850)
                     /gene="sodN"
                     /locus_tag="VM841_14960"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HVL91524.1"
                     /translation="MALRDWISPRTVHAHCDLPCGVYDPAQARIEAESVKAIMEKHNG
                     SDDAQFKARAIGIKEDRAEMVKHHLWVLWTDYFKPEHLDKYPNLHQLFWDATKAAGQA
                     KKSLDPADGQKLLDLINEIDKIFWDSKK"
     gene            2895..3269
                     /gene="sodX"
                     /locus_tag="VM841_14965"
     CDS             2895..3269
                     /gene="sodX"
                     /locus_tag="VM841_14965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HVL91525.1"
                     /translation="MVKHPVLGWAAGLGVAAGAALWLSRRYIPVKVQGPSMLPTLRPG
                     DWLIVSLKAKPRVGDIVVARRDDGLEIVKRVARIDDSGVELIGDNAPMSTDSRTIGVI
                     APDQIIGVAKPFLGIAASPTRR"
     gene            complement(3443..5221)
                     /locus_tag="VM841_14970"
     CDS             complement(3443..5221)
                     /locus_tag="VM841_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91526.1"
                     /translation="MTTMPKPVTDAQIRVLLRTLADEMPHDAPAPVAAVRRARGRLAR
                     NALAGLLAVAALAYGAISTTSRITTPKPQPPAVSPTAAGGGIWTMDAQGKNLVRLTAG
                     RDDAPAWSPDGEQIAFARAGRDGKSAVWVMNADGSDARKIIDEGSSPAWSADGTRIAF
                     EVLLNDRWTIFSVARDGSDDRLMTEGRFPAFSPDGTRFAYELGDERRLFLGTLGPDGL
                     SRSAGRVDTPGEAGLPLFLGNDRLAFYDIAERVYRVLDIRTGGHTRLHNADGDASPRR
                     RPGLGPNGSIVFTHGSNTVVGAFTATPSEIDLDGTRLTYDPSIHDGFPSWSPKAKRFV
                     LTRTVDPGTAVFVMDADGSNVSRLTEGTRIDGGPVMSPDGTKIAFASTRDGYAAIYVM
                     AADGGDQRAVTLSVNGHVRPIWTPDSLSLYVMNLTDGIAAGIGKTVPSMSTVSTIVEP
                     EPHMSPITLSADGTRLAYSYGTDESTRTLVVATSDGVMERGVVSGGVKAAAWGPAEQI
                     AFTDGTRLFVINADGSGRRALSSYAGENVAGLWWGTGGEILFTSSGDIRAMNPATGAV
                     RTIDLPGTQYNPSWTPDGRILFNGEQ"
     gene            complement(5205..5783)
                     /locus_tag="VM841_14975"
     CDS             complement(5205..5783)
                     /locus_tag="VM841_14975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HVL91527.1"
                     /translation="MDKRAQPFTQHACLSVVSTEDVAMTLEQAGVSGRLGELYLEHAP
                     ATARFAYLMTGDRALAEDLVQDAFARVIGRLGHLREASAFPAYLRRAVVNLAKNHYRR
                     RSIEQAYLEREGARPSQHITGPDLAAADAMRRALLALPERQRAAIALRFYEDLSEEQI
                     AAALECRPGTVRSLVSRGMATLREQLGGDDDA"
     gene            5810..>5968
                     /locus_tag="VM841_14980"
     CDS             5810..>5968
                     /locus_tag="VM841_14980"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HVL91528.1"
                     /translation="MNRRTTIEIDERLLERAKRALGLRTTRATVEEALRRVAADAEDA
                     RARVSEEQA"
BASE COUNT          959 a         1942 c         2041 g         1026 t
ORIGIN      
        1 gcccataacc cctggacccc tgtgcaacca agttgcatga atgcgtcccg gcgagtccat
       61 gcaacttggt tgcacagggg tccaggggtt atgggcggcg tctcgcggcg cggggccgat
      121 attctggggc ggtgaccgct cccgagatcg cggcgcgcct gcgcgagatg atccatctgc
      181 cacaagaggt ggccgagctt gcccatcgct tcgtcaagca gggcttcgag ctgtatctgg
      241 tcggcggcag cgtgcgcgac gcggttctgg gccgcctgca cgacgatctg gattttgcga
      301 ccagcgcgaa gccgaacgag atcttggagg tcgtgcgcgg ctgggcggat gcgacctggc
      361 tgaccggcat cgagttcggc accgtcggcg tcgcgcggcg cggtcatcgc ttggagatca
      421 cgactttccg cggcgagaag taccgcgaag attcgcgcaa gccgctgatc gagacggtct
      481 cgacgatcga agcggatctg gcgcggcgcg acttcacgat caacgcgatg gcgctgcggc
      541 tgcccgaacg cgcgctggtc gatccacacg gcggcatcgg cgacctggcc gcgaaagtgc
      601 tgcgcacgcc gctcgcgccg gaggtgtcgt tcagcgacga tccgctgcgc atgctgcgcg
      661 cggcgcggtt cgcggctgtt ttggattgtg tgccggtgcc cgagctggtc gaagcgatga
      721 ccgcgcagcg cgcgcgcctg aagatcgtgt cggccgagcg gatccgcgac gagctgtcga
      781 agctgctcgc ggcgcccaag ccctcgatcg gcctggatct agcgacgtcg gcgggactgt
      841 gcgacctgtt cctgcccgag ctgccggcgt tgaagctgga gcaggatccg gttcaccggc
      901 acaaggacgt gtatcgccac acgctcgcgg tcgtggacaa gctggccgcc ggcgatcccg
      961 agcagcagcc cgacgtgctg ctgcgcatgg ccggcctgct gcacgacatc gccaaacccg
     1021 cgacgcgccg cttcgacaag ggcaaggtca cgttccacca tcacgaggtc gtcggcgcgc
     1081 agatgaccgc caaacggctg cgcgcgctgc gctatccggg cgagttcgtg gacgactgcg
     1141 ccgcgctcgt cgagatgcat ctgcggttcc acacgttccg gatgggctgg agcgacagcg
     1201 ccgtgcggcg atacgtgcgc gacgccggcg gcctgctgga ccggctcaac cgtttggtgc
     1261 gcgccgactg caccacccgc aacgcgctca aagccaagcg gctgtcggat gcgatggacc
     1321 agctcgagtc gcgcatcgcc gagctggcgt cgcaggagga tctgaagcgg atccgtccgc
     1381 cgctcaacgg ccacgaggtc atgcagtatc tggcggtgcc gccgggtccc gcggtcggtc
     1441 gtgccttgga tcatctgctc gagatccgcc tggatcgcgg cgaatattcg aaggacgagg
     1501 cctatgcgat gctcgacgcc tggaaggccg agcatctgta aaagccgcat ttggccattc
     1561 cgggccatat cgcgctcgtt cgtgggcagg tagggtagag aagcaaggtt cgacgagcga
     1621 aagtggtgat gatcgtggcg atgtccgaac ccgacggtgc ccgcacgccg caaacgccga
     1681 cgactgcggg acagcgcgac gccgagcaag atcagcagca tctgcgcgtg atcgcggccg
     1741 aacgccgccg gcgagcgatc aaactgggtg ccctcgccac gctcgtcgtg ctgttcgcga
     1801 tcttcgtcgt gcagaactcg cagcccgctc ccgttgattt catcttcgtg cagagcagcc
     1861 cgcggctgat ttggatcatg ctggtctgcg ccgtgatcgg cggagtgatc ggctacatct
     1921 tgggccgccc cggaaagacg ctgcgcttcc accgcaaaga cgacaagcag cagaagccgg
     1981 cccgctaagg gcggcgaatc ggatccagag ggcacagcgt cggctgcagg gccgacgttg
     2041 cgcgatagac ctcgccgatc tcgaccccaa agccgcgtcc ggttgcctca tatggtggcc
     2101 cggatacggc ggggccgatt cccgggttgt cggggccgca tccgatgctg ccgccaacgc
     2161 ccatggggcc gaagaagtcc acacccggcg tgaacagcac gtcgaattgt tctccttcga
     2221 gcgtcgcgcg gatccgcacg ctcccgtcga gatgttcctc gaaggcgtag cgggatggac
     2281 tcccggacac gacacggaaa tcgccgcggc ggcacgagac gccggacgag ggtccttctg
     2341 cggagtgtcg atattggtcc cggacgggag cggaaaggcg tctcgcgcct gtggcggtaa
     2401 cgcgaacaag acgcgagggg ccccgtcatc cggagcccct cagcgtgatg atgatcgcta
     2461 cttcttggag tcccagaaga tcttgtcgat ctcgttgatc aggtcgagca gcttctggcc
     2521 gtcggccggg tcgagggact tcttggcctg gccggccgct ttggtggcat cccagaagag
     2581 ctggtgcagg ttcggatact tgtcgaggtg ctcgggcttg aagtagtcgg tccacagcac
     2641 ccacaggtgg tgcttgacca tctcggcccg gtcttccttg atgccgatcg cccgcgcctt
     2701 gaactgcgcg tcgtcgcttc cgttgtgctt ctccatgatc gccttgaccg actcggcctc
     2761 gatgcgagcc tgcgccgggt cgtacacgcc gcacggaagg tcgcagtgcg cgtgcaccgt
     2821 ccgcggcgag atccaatcgc gaagagccat agacatctcc ctccttcttt gctgttggta
     2881 taccatcgcg cgccgtggtg aagcatcctg tgctcggatg ggctgccggc ctcggggtcg
     2941 cggccggggc ggccctgtgg ctgagccgcc gctatatccc cgtcaaagtt cagggcccca
     3001 gcatgctgcc gaccttgcgg ccgggagatt ggctgatcgt gtcgctgaag gccaaaccca
     3061 gggttgggga catcgtcgta gctcgccgag acgatggcct ggagatcgtc aagcgagtcg
     3121 cgcggatcga cgactccggt gtcgagctga tcggtgacaa cgctccgatg agcaccgact
     3181 cgcggacgat cggagtgatc gcacccgatc agatcatcgg cgtagcgaag ccatttttgg
     3241 ggatcgcggc ttcgcctacc cgccggtaag tagcgacgct cgcgtgcggt tgaaacaaac
     3301 gaaccgaccg ctgcgatccg gggtcccgtc catggaacga atcggctgct ctttcgcacc
     3361 gctcgcagca tctcggcttt ggcggcgcga gttcgtgctg ccgaggatct tggctacgag
     3421 accgccggcg gtgcgtccgg cgctactgct cgccgttgaa caggatgcgg ccgtcggggg
     3481 tccagctcgg gttgtattgc gtgcccggaa ggtcgatggt gcgcacggcg cccgtcgccg
     3541 ggttcatcgc gcggatgtct ccgctgctgg tgaacaggat ctcgccacct gttccccacc
     3601 acaggccggc aacgttttcg cccgcatacg agctgagcgc gcggcggccc gatccgtcgg
     3661 cgttgatgac gaaaagccgc gtgccgtcgg tgaaggcgat ctgctcggca ggaccccacg
     3721 ccgcggcctt gaccccgccg gacacgaccc cgcgctccat gaccccgtcg gacgtggcaa
     3781 caaccagtgt tctcgttgat tcgtccgtac cgtaggagta cgccagtctg gtcccgtccg
     3841 cggacagcgt gatcgggctc atgtgtggct cgggttcgac gatcgtgctg accgtggaca
     3901 tcgacggaac ggtctttccg atgccggcgg cgatgccgtc ggtcaggttc atcacgtaca
     3961 ggctcaagct atcgggggtc cagatcggac gaacgtggcc gttgaccgac aaggtgacgg
     4021 ctcgctggtc gcctccgtcg gcggccatca catagatcgc cgcgtatccg tcacgtgtgc
     4081 tcgcgaacgc gatcttcgtg ccgtcgggcg acatgaccgg gcccccgtcg atccgcgtgc
     4141 cctcggtcag ccgcgacacg ttcgatccgt cggcgtccat cacgaagacc gcagtgccgg
     4201 gatcgaccgt gcgcgtcaac acgaaacgtt tcgccttggg actccacgac gggaacccgt
     4261 cgtggatcga cggatcgtac gtgagtcgcg ttccgtcgag atcgatctct gatggcgtcg
     4321 ctgtgaacgc gccgacgact gtgttcgagc cgtgggtgaa gacgatgctg ccgttcgggc
     4381 cgaggccggg gcggcgccgc ggcgaggcat cgccgtcggc attgtgcagg cgtgtatggc
     4441 cgccggttcg aatgtcgagg acgcgataga cgcgctcggc gatgtcgtag aacgcgagcc
     4501 ggtcgttgcc gaggaacaac ggcagtccgg cttcccccgg cgtgtccacg cggccggcgc
     4561 tcctggacag gccatccggg cccagcgtcc cgaggaacag gcgtcgctca tcgccgagct
     4621 cgtatgcgaa gcgcgttccg tcgggcgaaa acgccgggaa ccgtccttcg gtcatcaggc
     4681 gatcgtcgga gccgtcccgt gccaccgaaa agatcgtcca ccggtcgttc agaagaacct
     4741 cgaaggcgat ccgcgtgccg tccgcggacc acgccgggct ggatccttcg tcaatgatct
     4801 tgcgcgcgtc cgagccgtcg gcgttcatca cccagacggc gctttttccg tctctgccgg
     4861 cccgcgcgaa ggcgatctgc tcgccgtcgg gagaccaggc gggcgcgtcg tcgcggcccg
     4921 cggtcaagcg caccaggttc ttgccctggg cgtccatcgt ccagattccg ccgccggccg
     4981 cggtcggaga caccgccggg ggttgcggct tcggcgtcgt gatgcgcgac gtcgtcgaga
     5041 tcgcgccgta cgcgagcgcg gcgacggcaa gcagccccgc gagcgcgttg cgtgccagcc
     5101 ggccgcgggc gcgacgcacc gctgcgacgg gtgcgggtgc gtcgtgtggc atctcgtcgg
     5161 cgagcgttcg cagcagcacg cgaatctgcg cgtccgtgac cggtttaggc atcgtcgtca
     5221 ccaccaagct gctctcgaag ggttgccatg ccgcgggaga ccagcgaccg gacggtcccg
     5281 ggccggcatt ccagtgcggc ggcgatctgt tcttcactga gatcttcgta gaagcgcagc
     5341 gcaatcgcgg ctcgctgccg ttcgggcagc gccagcaggg cgcgacgcat cgcgtcggcg
     5401 gcggcgaggt cagggccggt gatgtgctgg gacgggcgcg cgccttcgcg ttcgaggtac
     5461 gcctgctcga tcgagcggcg gcggtaatgg ttcttcgcca ggttgacgac cgcgcggcgc
     5521 agatacgccg ggaacgcgct cgcctcacgc agatgcccca gccgcccgat gacccgcgcg
     5581 aacgcatcct gaacaagatc ctccgccagc gcccggtccc cggtcatcag gtaggcaaac
     5641 cgggccgtcg cgggcgcgtg ctcgaggtag agctcgccca gccggcccga aacacccgcc
     5701 tgctccaggg tcatcgccac atcttctgtg ctcacaacag aaagacacgc gtgctgcgtg
     5761 aatggctgcg ctcgtttatc catacatata gaattgatag tatgtacata tgaatcggcg
     5821 aacgacgatc gagatcgatg agcgcttgct cgagcgagcc aagcgggcgc tcgggctgcg
     5881 gactactcgc gccactgtcg aggaggcgct gcggcgggtg gccgccgacg ccgaggacgc
     5941 aagagcccgc gtctcggaag agcaggcg
//
LOCUS       DATLFO010000366         4147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_138286, whole genome shotgun sequence.
ACCESSION   DATLFO010000366 DATLFO010000000
VERSION     DATLFO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138286"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VM841_14985"
     CDS             <1..515
                     /locus_tag="VM841_14985"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HVL91529.1"
                     /translation="VEAQTIPIIREGIFQEFISSRETAGETGGERSNGTMRASGWDVI
                     PLIRMTNINLEPGTSSLEEMIATTDDGILMSTNQSWSIDDKRVNFQFGCEIAWEIKDG
                     KRGRMLRNPNYAGRTVDFWSSCDALGGPDEWVVWGTPNCGKGQPGQTARVAHGTSPGR
                     FRGVNVGVRG"
     gene            512..1864
                     /locus_tag="VM841_14990"
     CDS             512..1864
                     /locus_tag="VM841_14990"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVL91530.1"
                     /translation="MSRLLGPDRLADAIEPALRSAADGTLVVAVHSAGGLTRFASSRI
                     HQNTHREDVEIRVMAVADECRVGVATTHSLERERVEAASANALSIARVTPRTAAFPGI
                     TEPRPLAAELPERFDEATAATSPAERAAAVTLALNEFPSDMEGAGAVETVADEVLVAT
                     STGVRHYGRITRAAVSVMAMSPDSSGYAELIEGKLSDLDAAAIGARAVRKAEAGRNPQ
                     PVGPGSYTVILEPAATSTLMQFLAWLGFGAKSLLEERSFMAGKIGQKLMDERITITDD
                     PLAADALGLPFDFEGTPAQPVTLIDRGVATSVVWDRETAKRGGRESTGHGLPAPNPDG
                     PFPLNLRMQPGTDSLDDLIAGCERGLLVTRFHYSNVVHEKEAILTGMTRDGTFLVQDG
                     KILHGVRNLRYTQNAVEALRNVEAIGDTTEISSELFFGGSRAPALRIRDFKFSSTTDH
                     "
     gene            1867..2295
                     /locus_tag="VM841_14995"
     CDS             1867..2295
                     /locus_tag="VM841_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007620146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVL91531.1"
                     /translation="MTAPLCAVGAIVMDKGRLLMVLRDREPLKGCWTLPGGRLETGES
                     MHEAIVREVREETGIDIDVDGLLGVVERIVRNDDGEVSYHYVILDFVATPRGTAIKAG
                     DDAAEVRWVPVGELSSLKLTDGLIEFLSDRGILEGRKPRL"
     gene            complement(2370..2732)
                     /locus_tag="VM841_15000"
     CDS             complement(2370..2732)
                     /locus_tag="VM841_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91532.1"
                     /translation="MQPMPVRTGPPSRMTRGRRTGIVILLIFGALFYLQFGLQSLTMH
                     MLQAVSVLIGAAGLGWAAAAAIRRRPAAGIALWGTMPALLVHAGMTIQDSGELPFLIG
                     SIPAPLIAGGALLIRARR"
     gene            2873..3652
                     /locus_tag="VM841_15005"
     CDS             2873..3652
                     /locus_tag="VM841_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91533.1"
                     /translation="MPRLTIHDFRVTDSILAQTETDAGDVVFLGPNGTTAFPFVVWRK
                     VSGPGGVYVDAFEIAGPDGFVVGPWERSFEVDGESWPMDVVTEVRDWKFPAAGDYTLR
                     YYEYDDKIFEVPFQVVQQDPPYGVVVPGPLDAALSKSTLCWLDVRQDDGSVKSFGVWF
                     GYEDGRVYVLFGGDGEQEVTGLERAKYVRLIARSKDVQSRIGEVDCAVEILAKDARWD
                     KLVQELLIGRRLNVKDPVNAPKRWREQCEIAMLTPVAAPVS"
     gene            3649..4071
                     /locus_tag="VM841_15010"
     CDS             3649..4071
                     /locus_tag="VM841_15010"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HVL91534.1"
                     /translation="MTTQDSLPTVLFVCIHNAGRSQIAEALFNAYSGGKARAVSAGVE
                     PVQALNVKVVAAMRDAGYDMGGQAPKLLDPAMIDAADRVITMGCMDDPSCPAVLADED
                     WGLDDPAAAPMPKVVAIRDEIARRVRALLREMGIEPVR"
BASE COUNT          696 a         1425 c         1425 g          601 t
ORIGIN      
        1 gcgtcgaggc gcagacgatc ccgatcatcc gcgaaggaat cttccaagag ttcatcagct
       61 cgcgcgagac cgcgggcgag acgggcggcg agcgttccaa cggcacgatg cgcgccagcg
      121 gctgggacgt catcccgctg atccgcatga ccaacatcaa cctggagccg ggcacgtcct
      181 cgttggagga gatgatcgcc accaccgacg acggcatctt gatgtccacc aaccagtcgt
      241 ggtcgatcga cgacaagcgc gtcaacttcc agttcggctg tgagatcgcc tgggagatca
      301 aagacggcaa acgcggccgg atgctgcgca acccgaacta cgcgggacgc accgtggact
      361 tctggtcttc gtgcgacgcg ctgggcggcc cggacgagtg ggtcgtgtgg ggaacgccga
      421 actgcggcaa gggacagccg ggccagaccg cgcgtgtcgc gcacggcacc tcacccggcc
      481 gcttccgggg cgtcaacgtg ggagtgcgcg gttgagccgc cttctcggac cggaccggct
      541 agccgacgcg atcgagccgg cgctgcgctc cgctgccgac ggcacgctcg tcgtcgcggt
      601 gcacagcgcg ggcggcctga cccgcttcgc gtcgagccgc atccatcaga acacgcaccg
      661 cgaggacgtc gagatccgcg tgatggccgt cgccgacgaa tgtcgcgtcg gcgtcgcgac
      721 cacgcattcg ctggagcgcg agcgtgtcga ggcggcgagc gccaacgcgc tgtcgatcgc
      781 acgcgtgacg ccccgcaccg ccgcgttccc cgggatcacc gagccgcgcc cgctggccgc
      841 ggagcttccc gagcggttcg acgaggcgac ggccgcgacc tcgccggccg agcgcgccgc
      901 cgcggtcacg ctcgcgctca acgagtttcc ctccgacatg gaaggcgcag gcgcggtcga
      961 aaccgtcgcc gacgaggtgc tcgtggcgac ctcgacgggc gtgcgccact acgggcgcat
     1021 cacgcgcgcc gcggtcagcg tgatggcgat gagccccgac tccagcggct atgccgagct
     1081 gatcgaaggc aagctgagcg atctggacgc ggccgcgatc ggcgcgcgcg cggtccgcaa
     1141 ggccgaggcg ggccgcaacc cgcagccggt cgggcccggc tcctacaccg tcatcctcga
     1201 gccggccgcg acctcgacgc tgatgcagtt tttggcctgg ctcggcttcg gggccaagtc
     1261 gctgctggag gagcgctcgt tcatggccgg caagatcggc caaaagctga tggacgagcg
     1321 catcacgatc accgacgatc cgctggccgc cgacgcgctc gggctgccgt ttgacttcga
     1381 aggcacgccc gcgcagccgg tcaccctcat cgacaggggc gtcgccacga gcgtggtctg
     1441 ggaccgcgag acggccaagc ggggcggccg cgaatccacc ggccacggcc tgcccgcccc
     1501 caacccggac gggccgttcc cgctgaacct tcgcatgcag cccggcaccg attcgctcga
     1561 cgacctgatc gccggctgcg agcgcggcct gctggtcacg cgcttccact actccaacgt
     1621 cgtgcacgaa aaagaagcca tcctgaccgg catgacccgt gacggcacgt tcctggtgca
     1681 ggacggaaag atccttcacg gcgtgcgtaa cctgcgctac acgcaaaacg ccgtcgaggc
     1741 gctgcgcaac gtcgaggcga tcggcgacac gaccgagatc agctccgagc tgttcttcgg
     1801 cggctcgcgc gcacccgcgc tgcgcatccg cgacttcaag ttctcgtcca cgacggatca
     1861 ctaacgatga cggctcccct atgcgccgtc ggcgcgatcg tgatggacaa gggccgcttg
     1921 ctgatggtgc tgcgcgaccg cgagccgctc aagggctgct ggacgcttcc gggcggccgg
     1981 ctcgagacgg gcgagtcgat gcacgaggcg atcgtgcgcg aggtgcgcga ggagaccggc
     2041 atcgacatcg acgtggacgg cctgctgggc gtggtcgagc gcatcgtgcg caacgacgac
     2101 ggcgaggtga gctaccacta cgtgatcctg gacttcgtcg ccacgccccg cggaaccgcc
     2161 atcaaagccg gcgacgacgc ggccgaggtg cgctgggttc cggtcggcga gctgtcctcg
     2221 ctcaagctaa ccgacggcct gatcgagttc ctctccgacc gcggcatcct cgaaggccgc
     2281 aagccccgcc tgtaagacct attcatcacg accaaggcca gcggccctcg gtttggtgag
     2341 cgggctggaa acgagcgcga gcgtcgcgtt tagcgccggg cgcggatcag cagcgcgcca
     2401 ccggcgatca gcggggcggg aatgctgccg atcaaaaacg gaagctctcc cgaatcttgg
     2461 atggtcatgc ccgcgtgaac cagcagagcg ggcatcgtgc cccaaagcgc gattcccgca
     2521 gcgggacggc gccggatcgc ggccgccgcg gcccacccca gtcccgccgc gccgatcaga
     2581 accgatacgg cttggagcat gtgcatcgtc agcgactgca gcccgaactg caggtagaac
     2641 aaggcgccga agatcagcag gatcacgatg ccggtccgcc gcccgcgggt catgcggctg
     2701 ggcgggccgg tgcgaacggg catcggctgc attggcaagg ctccttcatc ggaggcgtgc
     2761 gcggattcta gccgccggag aggtccggca gacccgtgtg atgcccccgg ggatgcgcgc
     2821 cgatataatc ccgccgccga aatcgaacgc gattcacaaa ggggcgacca tcgtgccgag
     2881 gctgaccatc cacgacttcc gcgtcaccga ctcgatcctg gcgcaaaccg agaccgacgc
     2941 gggtgacgtc gtcttcctgg gccccaacgg caccaccgcg ttcccgttcg tggtctggcg
     3001 caaggtcagc ggtccgggcg gcgtctacgt ggacgccttc gagatcgccg gacccgacgg
     3061 cttcgtggtc ggcccctggg agcgctcgtt cgaagttgac ggcgagtcct ggccgatgga
     3121 cgtggtcacc gaggtgcgcg actggaagtt ccccgccgcc ggcgactaca cactgcgcta
     3181 ctacgagtac gacgacaaga tcttcgaggt tcccttccag gtcgtgcagc aagacccgcc
     3241 ctacggcgtg gtcgtgccgg gcccgctcga cgcggcgctg tcgaaatcga cgctgtgctg
     3301 gctggacgtt cggcaggacg acggcagcgt caagtcgttc ggcgtctggt tcggctacga
     3361 ggacggccgc gtctacgtgc tgttcggcgg cgacggcgag caggaggtca cgggactcga
     3421 acgggccaag tacgttcgtc tgatcgcgcg cagcaaagac gtgcagtcgc ggatcggcga
     3481 ggtggactgc gcggtcgaga tcctggcaaa ggatgcgcgc tgggacaagc tggttcaaga
     3541 gctgctgatc ggccgccggc tgaacgtgaa ggatccggtg aacgcgccca agcgctggcg
     3601 cgaacagtgc gagatcgcga tgctcacgcc ggtcgccgcc ccggtgtcgt gaccacgcag
     3661 gattccctcc ccaccgttct gttcgtctgc atccacaacg ccggccgctc gcagatcgcg
     3721 gaagcactgt tcaatgcgta ctcgggcggc aaggcccgcg ccgtctcggc gggcgtcgaa
     3781 ccggtgcagg cgctcaacgt caaagtcgtc gcggcgatgc gcgacgccgg ctacgacatg
     3841 ggcgggcaag ccccgaaact tctggatccc gcgatgatcg acgcggccga ccgcgtgatc
     3901 acgatgggat gcatggacga cccgtcctgc cctgccgtcc tggccgacga agactggggc
     3961 ctggacgatc ctgccgccgc gcccatgccg aaggtcgtcg cgatccgcga cgagatcgcg
     4021 cggcgcgtcc gcgcgctgct gcgcgagatg gggatcgagc cggtccgcta ggccggaacg
     4081 atcgtggcgc gcgcgacgcg gcgctcgtcg gagtccacga acgcctcgtt gatcttctcc
     4141 agcggat
//
LOCUS       DATLFO010000367        13726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_79200, whole genome shotgun sequence.
ACCESSION   DATLFO010000367 DATLFO010000000
VERSION     DATLFO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79200"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1240
                     /locus_tag="VM841_15015"
     CDS             248..1240
                     /locus_tag="VM841_15015"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVL91535.1"
                     /translation="MGKRTILAGVAAAMLLSVALPASAAGTVYESAVNLDITKRVSAA
                     LRQAGVTTVLTRWWDETRTPEQRVWVAERFRTDAFVSIHNNGSSNRSATHSEVYHQIA
                     GGTSKTLAQAIVAELPAATGERSYVKSRRGEKGDYYWQLRMNRRPSVIVESAFMSNRR
                     QAWLLGTSAAYRQAIADSVTRGILAWQRSLGATPPALDEPSLRVPTDLVPPPASVAAA
                     VQSRYRVGLRWTASAQATAYHVRRDGTLIAIVENPDATGVAGLLDSATMPMSFTDRWA
                     APGQTYEYEIVAVRTEGPATLESPPSLVRAKTPAISVCLDAGHGGADPGALGSW"
     gene            complement(1336..1743)
                     /locus_tag="VM841_15020"
     CDS             complement(1336..1743)
                     /locus_tag="VM841_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVL91536.1"
                     /translation="MILDTSVLVAAADRRDAAHRACADLLAGWAGPLFVPVLVAGEAA
                     WMIERRAGLMAEYRFIAELAAGSLTVGAIEPGDWLRIGELVEEYADLRLGTVDASIVS
                     VAERLGITTIATLDHRHFSVVRPAHTERFTLVP"
     gene            complement(1740..1997)
                     /locus_tag="VM841_15025"
     CDS             complement(1740..1997)
                     /locus_tag="VM841_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVL91537.1"
                     /translation="MKRTTIKLPDDLDALLRDEARRRGVSMSDVTREAIEAYLIPRGP
                     RRLSFVGAGASNDGRSIAEHFDEIYAEAMEERFRRKMGRDV"
     gene            complement(2017..2703)
                     /gene="radC"
                     /locus_tag="VM841_15030"
     CDS             complement(2017..2703)
                     /gene="radC"
                     /locus_tag="VM841_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HVL91538.1"
                     /translation="MPVRSVSVRDRPPADRPRERLAAHGASVLADHELLALVLRNGSA
                     AEGVVALAQRLLADAGGVGGLSRLGPRELARVPGVGEAKAAGVVAAFELARRSMAAMA
                     MERAQITGPADAAALLVPRLAHLDHEESIVLVLDRRHRLLRGVVVGVGGVAHAPMEPR
                     EVLQAALREPAAATVLVAHNHPSGDPEPSSDDLAVTQRLEQAAKLVGLDFLDHLIVAS
                     RGWRSLRTGS"
     gene            complement(2788..3198)
                     /gene="ndk"
                     /locus_tag="VM841_15035"
     CDS             complement(2788..3198)
                     /gene="ndk"
                     /locus_tag="VM841_15035"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HVL91539.1"
                     /translation="MSERTFVMVKPDGVRRGLVGEVVWRLERKTLKIIGMKLFTIDAE
                     LAETHYGEHKEKPFFGDLVSFITSGPVVAMCVEGEEAVSVCRTLMGATDPKKAAPGTI
                     RGDLGLAITENIVHGSDSAESAKRELGLFFPELT"
     gene            complement(3480..4769)
                     /locus_tag="VM841_15040"
     CDS             complement(3480..4769)
                     /locus_tag="VM841_15040"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HVL91540.1"
                     /translation="MRYEQALAALDERSNYEATGALVAPGLERMQALMDLLNGPQASF
                     PSIQITGTNGKTTAARVAASVLREIGLGVGTYTSPHLHSPRERMVYDREPISEAEFAD
                     TFAYLEPYLRTVDERHGPVTWFEAVTAMANVWFAERAVEAAVVEVGMGGSWDATNLVN
                     GRVALITEVAVDHPELGATPVEIAREKAGIIKRGAVCVTGERDPAVLEVIRRRCGEVG
                     ATLRRIDAEFAIEKRALAMGGTAMTLRVGERVYRDVFLPLFGAHLAHDAAVALAGVVA
                     FLGDRDLHDGVVANAFAAVTDPGRVEVVQRRPLIVLDGAHNPDAARALAETMAESFQF
                     DRLTLVAGMLAGKDVEGTMSPLAALATEVIATVPPSGRAVPAEVVAAAVEAFGTPVRV
                     IEDPRAAFTAAVEGAGDRDAVLVAGSLYLVAAVRGRA"
     gene            complement(4769..7396)
                     /locus_tag="VM841_15045"
     CDS             complement(4769..7396)
                     /locus_tag="VM841_15045"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HVL91541.1"
                     /translation="MGTELPKAYDPATVESRLYDTWEAAGLFEPHGDGQPFCIVLPPP
                     NVTGSLHMGHALDHSLQDAIVRRKRMQGFRVLWLPGTDHAGIATQNVVERELAGEGLS
                     RHDLGRDAFLERVWAWKEKSGSRITHQMRRLGDSCAWSYERFTMDEGLSRAVREIFVR
                     WYERGLIYRGNRITNWCPRCHTALSDIEVEHRDVDGELVQLRYPLSDGSGHMTIATTR
                     VETMLGDTAVAVHPDDERYKDLIGKTVTLPLVGREIPIIADEAIEMEFGTGALKVTPA
                     HDPVDFEIGRRHGLDAINVLSDDGTVNELGADFAGQDRFEARKNVLEALRSAGAVGHE
                     ERPFTHAVGHCYRCGTEVEPWLSEQWFVKIEPLATPAIAAVKDGRTRYVPERYARNYL
                     DWMENLRDWCISRQLWWGHRIPVWYCENGHQFASRTDPDACAECASASIRQDEDVLDT
                     WFSSALWPFSTLGWPDDTEELRTFYPNDVLVTGYDIITFWVSRMMMDGIDMMGDVPFR
                     DVHIHGMVRDFRGKKMSKSFGNVMDPLDLIERYGADATRMTLFRSAVLGGDVPVSEQW
                     VEGDRNFANKLWNLTRFVLMYAGEPQERPLPPADRQTVADRWILARLAAVTAEADAAM
                     EGYDFATAAVTLRNFAWNDFADWYVEWSKGRLQGGSEDQKQDTLAILTHVLSATLRLL
                     HPIMPFVTEELHGALSGTATILRGPWPAALAAEAGAVERFEFLKQIVAVLRRFRADHK
                     VPPQIKPAATVVVRDETMRALLQSELERVRVLGSWGEIALAAAAPDATGEARIVLAAA
                     TIHVPLAGVLDIDAEVARLRREIGDLEAEASKIEAKLANESFVAKAPEEVVEKQRERV
                     AEARAAIGGLAEALAELTA"
     gene            complement(7563..8054)
                     /locus_tag="VM841_15050"
     CDS             complement(7563..8054)
                     /locus_tag="VM841_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91542.1"
                     /translation="MTTHPAGSTKAGLRIRMLAVALSGLAFLPSAGLALPGESHLNEE
                     CRHWGVSTDEADVAVDCQDLVDTANVLTLKVTGIDCPALRRGDCHERADRVRNAATGA
                     LAPHACGDLGGAATHGCRKTRESTLFAYRLAMGLVFGPIGIVADAFCAGADPTGTAVT
                     SCR"
     gene            complement(8060..8512)
                     /locus_tag="VM841_15055"
     CDS             complement(8060..8512)
                     /locus_tag="VM841_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91543.1"
                     /translation="MHLKLIAALLAALAVAPATGAAAPAASQISDSCREYRADRDDLH
                     VSASCRAAARAADILVKDLTGVDCPVLSRGQCDEDAEQARREAFAALAPHICGDHPGF
                     TVRCDETRSDLVGAWRLAMGAAFAPARKFHERFCAGVDPTGTVETSCA"
     gene            complement(8624..9904)
                     /gene="clpX"
                     /locus_tag="VM841_15060"
     CDS             complement(8624..9904)
                     /gene="clpX"
                     /locus_tag="VM841_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HVL91544.1"
                     /translation="MAKFGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELNESTELKLEELPKPKEIYEFLNDYVIGQDEAKKVLSVAVYNHYKRIQVGAYH
                     DGEVELQKSNILLLGPTGCGKTLLAQTLAKMLNVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADYDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPHQEFIQIDTTNILFICGGAFAGLEKVIESRTGRKGVGFGADIRRADE
                     KNVGNILGKVMPEDLLRYGLIPEFIGRLPVITAVNNLDREALVKILTEPKNALVKQYR
                     KFFEFDGVDLSFDEDALDAVADEAMKRGTGARGLRAIMEEVLLGVMYDLPSREDVGKC
                     VISRESVVEHLPPRLEPKDGAAEPPAKIRRERSA"
     gene            complement(9925..10551)
                     /gene="clpP"
                     /locus_tag="VM841_15065"
     CDS             complement(9925..10551)
                     /gene="clpP"
                     /locus_tag="VM841_15065"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HVL91545.1"
                     /translation="MQNYLVPTVIESTNRGERAFDIYSRLLKERIVFLGTEVDDAVAN
                     LIMAQMLHLESEDPDKDIHLYINSPGGSITALFAIYDTMKYIKPHTSTICMGQAASAA
                     AVLLASGASGKRYALPHARILIHQPHGGAQGQAVDIEIQAKEILRYRRLLDQILAEHT
                     GQDLEKISKDTDRDFIMTAEEAKAYGIIDEVISSRHAQDKLEKTAAAS"
     gene            complement(10640..11944)
                     /gene="tig"
                     /locus_tag="VM841_15070"
     CDS             complement(10640..11944)
                     /gene="tig"
                     /locus_tag="VM841_15070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVL91546.1"
                     /translation="MQTTVSREGPTRIRMTVEVAPDEIKPAADRAFKQLAKQVKIPGF
                     RPGKAPRSLLEARLGKDEVKEMIVREAVPQFYAQAVVGEDIDPVAPPQIEVTSFEVDE
                     PLIFEAIVEVRPEIDLPDINGLKVTRPPFAATDEDVQNQLERLADRFASLETVERPAT
                     EGDFVLIDIKGYWNDEEIDNATANDMLYEAGSGRIVPELDAEVLGKKAGDILKFNAEL
                     PPTFGSTWGGREVTFQVLVKEVRQKNTPALDDEFAKTASEFETLGELKADLADKITRI
                     KRSGADAEVRSRMLDVLVNAVPVAAPDAMVDEEVAYRLHRLSDQLRGAGVSLDDYLTS
                     AEATEQEIEADIRKQAERSVSAQLILEEIARREELTVEEAELDEEIGRLAEASGENGP
                     ELRKRLEQAGRVGVVAGDILRRKALDLVVEKADIKEENADAE"
     gene            complement(12024..12099)
                     /locus_tag="VM841_15075"
     tRNA            complement(12024..12099)
                     /locus_tag="VM841_15075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12061..12063),aa:Pro,seq:tgg)
     gene            12152..12222
                     /locus_tag="VM841_15080"
     tRNA            12152..12222
                     /locus_tag="VM841_15080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12184..12186,aa:Gly,seq:tcc)
     gene            12306..12379
                     /locus_tag="VM841_15085"
     tRNA            12306..12379
                     /locus_tag="VM841_15085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12340..12342,aa:Arg,seq:tct)
     gene            12400..12476
                     /locus_tag="VM841_15090"
     tRNA            12400..12476
                     /locus_tag="VM841_15090"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12434..12436,aa:His,seq:gtg)
     gene            12584..12657
                     /locus_tag="VM841_15095"
     tRNA            12584..12657
                     /locus_tag="VM841_15095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12618..12620,aa:Lys,seq:ctt)
     gene            13007..>13726
                     /locus_tag="VM841_15100"
     CDS             13007..>13726
                     /locus_tag="VM841_15100"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HVL91547.1"
                     /translation="MAGIVAAAGAFSILMAVRPANAQQLATLKVLSGSVSVSVQGAAV
                     AAATDGMSVKAGDVVETGDPGRAVVRYFDGSETRLDSNTRFEVRELHTLPNKAQSKVI
                     RGKQTKGATFNRIVKLTDSQSRVQTEAPTSTASVRGTAYSGTSNADGTFTWRVFEGAI
                     KVTDSFGKSVIVKAGQGVIVDEDGRLLEVYDLSDEELQAEWVTFNNCKLDGMGCPQVL
                     DDKQTGGPGTPTDEPQGPQGPQ"
BASE COUNT         2136 a         4718 c         4611 g         2261 t
ORIGIN      
        1 gaggtcgatg gccatgtccc ggccgaggaa ctgcagcgag cgtccgaaca tacagggaag
       61 gttctttctc ccgggggagg gtcggcccgg atggcggccg gtacggcccc ggggtcggcg
      121 cgaatcttag catcgcgcca cagaccgaca aaaccgcctc cgaccaggga ggattccggc
      181 cccgccatgc ggaagtaggg cccgtgcccg ccccttcgac ggcgggaatg cggacgaatc
      241 ggtcttgatg ggcaagcgca cgatcctggc gggcgtcgcg gcggcgatgc tgctgtcggt
      301 tgccctgccc gcgagcgccg ccgggaccgt ctacgagtcc gcggtcaacc tcgacatcac
      361 caagcgggtg tcggccgcgc ttcgccaagc gggtgtcacc acggtactga cccgctggtg
      421 ggacgagacc cgcacaccgg agcagcgcgt ctgggtcgcc gagcggttcc ggaccgatgc
      481 cttcgtctcg atccacaaca acggttcgtc gaaccggtcg gccacccact ccgaggtcta
      541 tcaccagatc gcgggcggaa cgtccaagac gctggcgcag gcgatcgtgg ccgagcttcc
      601 ggccgctacc ggcgaacggt cctacgtgaa gtcccgccgc ggcgagaagg gcgactacta
      661 ctggcagcta cggatgaacc gccgtccgtc ggtgatcgtt gagtccgcgt tcatgtccaa
      721 ccgccggcaa gcctggctgc tgggcacctc ggccgcctac cggcaggcca tcgccgactc
      781 ggtcacgcgc gggatcctgg cctggcagcg gtctttaggt gcgacgccgc ccgccctgga
      841 cgagccgtcc cttcgcgtgc cgacggacct ggtcccgcca cccgcgtcgg tcgcggccgc
      901 cgttcagagc cggtaccgcg tcggattgcg gtggacggcc agcgcgcaag cgaccgccta
      961 tcacgtgcgc cgcgacggaa cgctgatcgc gatcgtcgaa aatcccgacg cgaccggtgt
     1021 cgccgggctg ctggattcgg ccacgatgcc gatgtcgttc accgaccgct gggccgcacc
     1081 cggacagacc tatgaatacg agatcgtcgc ggtgcgcacc gaaggaccgg ccacgctgga
     1141 gtccccaccg tcgctcgtgc gcgcgaaaac gccggcaatc agcgtatgcc tggatgccgg
     1201 ccacggcgga gccgacccgg gagctctcgg cagctggtag ttgggcgggc cagacggcgt
     1261 cgtccccttc gtacacgctc gcgggacgcc ttctggggca ggcctcgcgc ggcggggatt
     1321 cgacgtcgaa gagcgctacg gcacgagcgt gaagcgttcg gtgtgggcgg ggcgcacgac
     1381 ggagaagtgg cggtggtcga gggtggcgat cgtggtgatg ccgagtcgct ctgcgacgct
     1441 cacgatcgaa gcgtcgaccg tgccgagacg aagatcggca tactcctcca cgagttcccc
     1501 gatccgaagc cagtcacccg gctcgatcgc tccgaccgtc aatgagccgg cggcaagctc
     1561 ggctatgaac cggtattcgg ccatcaggcc ggcccttcgc tcgatcatcc acgcggcttc
     1621 gcccgccacc aagacgggaa cgaaaagtgg ccccgcccag ccggcgagca gatccgcgca
     1681 cgctcggtga gcggcgtctc gccgatctgc ggcggcaacc aggacgctcg tatcaaggat
     1741 cagacgtcgc ggcccatctt ccgccggaag cgctcttcca tcgcctcggc gtagatctcg
     1801 tcgaagtgct ccgcgatgct gcggccgtcg ttgcttgccc ccgcgcctac gaacgagagc
     1861 cggcgcggtc cccgggggat caggtacgcc tcgattgcct cccgagtcac gtccgacatc
     1921 gagacgccgc ggcgcctcgc ctcgtcccgc agcagggcgt ccaggtcgtc gggcagcttg
     1981 atcgtcgtcc gtttcatggt atgccagcat atcctatcag gagccggttc tcaggctccg
     2041 ccagccgcga gacgcgacga tcagatgatc caggaagtcc aggccgacca gcttggcggc
     2101 ctgctcgagc cgctgggtca cggccagatc gtcgctgctg ggctcggggt cgccgctggg
     2161 atggttgtgg gcgacgagga ccgtggcggc ggcgggttcg cgcagcgcgg cctgcaggac
     2221 ctcgcgcggc tccatcggcg cgtgcgcgac gccgcccacg ccgacgacca cgccgcgcag
     2281 cagccggtgc cggcggtcca gcaccagcac gatcgactcc tcgtgatcga ggtgcgcgag
     2341 ccgcggcacg agcagcgcgg ccgcgtcggc gggtccggtg atctgcgcac gctccatcgc
     2401 catcgcggcc atcgagcgcc gtgcgagctc gaaggccgca accacgccgg cggccttggc
     2461 ttcgccgacg ccgggaacgc gtgcgagctc gcgcgggccg aggcgcgaca ggccgccgac
     2521 accgcccgcg tcggccaaca gccgctgcgc gagcgcgacc acgccctcgg cggcgctgcc
     2581 gttgcgaagc acgagcgcga gcagctcgtg gtcggcgagc acgctcgcgc cgtgcgccgc
     2641 cagccgctca cgcgggcggt cggccggcgg ccggtctcgg acggatacgc ttcgcacggg
     2701 catggtcgca atcctgaggc gtcccgggcg cgaaatgaag cgatcccgcc cggatcgctc
     2761 cgaacgggat caacaagagc cttcgcgtca ggtcaattcc gggaagaaca gcccgagctc
     2821 gcgcttcgcc gactcggccg agtcgctgcc gtgcacgatg ttctcggtga tcgccaggcc
     2881 gaggtcgccc cggatcgttc ccggcgcggc cttcttgggg tcggtcgcgc ccatcagggt
     2941 gcggcagacg ctgaccgcct cttcgccctc gacgcacatc gccacgaccg ggcccgaggt
     3001 gatgaacgag accagatcgc cgaagaacgg cttctccttg tgctcgccgt aatgcgtctc
     3061 ggcgagctct gcgtcgatcg tgaacagctt catcccgatg atcttgagcg tcttgcgctc
     3121 gagccgccag acgacctcgc cgacgaggcc gcggcggacg ccgtcgggct tgaccatgac
     3181 aaacgtgcgt tcggacatgc tcgtgagttc ctccaggaga tgggaattgc cgcccgtctt
     3241 tgtagcatcc tccggccgcc gccgcgatcc ggcggccgga ggatccgcgg ggccgttcct
     3301 cccgctcatc ccgcgaccgg cgacaccgct ccaccgcgat gcgtgcgttg ccggcatcgg
     3361 cgacggagag gttgccgtcg ccgcccgatc gccggcccgg acgggtcgtc gagctcggag
     3421 atgccgaatt ggttcaagga gacgccaccg aggctgaaga cctggatgcg cgcggcaggt
     3481 caagcgcggc cgcgcacggc ggccacgaga tagagcgagc cggccacgag caccgcgtcc
     3541 cggtcgccgg cgccctcgac cgcggcggtg aacgcggccc gggggtcctc gatgacgcgg
     3601 accggcgtgc cgaaggcttc gacggcggcg gcgaccacct cggcgggcac ggcgcgtccg
     3661 ctcggcggca cggtcgcgat cacctcggtt gccagcgccg cgagcggcga catcgtgcct
     3721 tcgacgtcct tgccggcgag catgccggcg accagcgtca gccgatcgaa ctggaacgac
     3781 tcggccatgg tctcggccag ggcgcgggcg gcgtcgggat tgtgcgcgcc gtccagaacg
     3841 atcagcggcc gccgctgcac gacctcgaca cgccccgggt cggtgacggc ggcgaaggcg
     3901 ttcgccacga cgccgtcgtg caggtcccgg tcgccgagga aggccacgac gcccgccagc
     3961 gcgaccgcgg cgtcgtgtgc caggtgagcg ccgaagagcg gaaggaacac gtcccggtaa
     4021 acgcgctccc cgacccgcag ggtcatcgcg gtgccgccca tcgccaaggc tcgcttctcg
     4081 atcgcgaact cggcgtcgat ccggcgaagc gtggctccga cctcgccgca gcgccggcgg
     4141 atgacctcga gcacggcggg atcccgctcg ccggtgacgc acacggctcc gcgcttgatg
     4201 atgccggcct tctcgcgggc gatctccacc ggggtcgcgc ccagctcagg atggtcgacc
     4261 gcgacctcgg tgatgagagc gacgcggccg ttgacgaggt tggtggcgtc ccaggatccg
     4321 cccatgccga cctcgacgac ggccgcctcg acggctcgtt ccgcgaacca cacgttcgcc
     4381 atcgcagtga ccgcctcgaa ccacgtcacc ggcccgtggc gctcgtcgac ggttcgaagg
     4441 tacggctcga gataggcgaa cgtgtcggcg aactcggcct cggagatcgg ctcgcggtcg
     4501 tagaccatgc gctcgcgagg cgaatgcaga tgcggcgagg tgtaggtgcc gacgccgagc
     4561 ccgatctccc gcagcaccga tgcggcgacg cgcgctgccg tcgtcttgcc gttggtgccg
     4621 gtgatctgga tcgacgggaa cgaggcctgc ggtccgttga gcaggtccat cagcgcctgc
     4681 atccgctcca gaccgggcgc gacgagcgcg cccgtcgcct cgtagttcga tcgttcgtcg
     4741 agcgcggcga gcgcctgctc gtaccgcatc aagccgtcag ctccgcgagc gcctcggcga
     4801 gcccgccgat cgcggcgcgg gcctccgcca cgcgttcccg ctgcttctcc acgacctctt
     4861 cgggcgcctt ggcgacgaag gactcgttgg cgagcttggc ctcgatcttt gacgcctcgg
     4921 cttcgagatc cccgatctcg cgccgcaggc gcgccacctc ggcgtcgatg tcgagcacgc
     4981 cggcgagcgg cacgtggatg gtcgcagccg ccagcacgat ccgcgcctca ccagtcgcgt
     5041 cgggcgccgc ggcggcgagg gcgatctcgc cccacgaccc gagcacgcgc acgcgctcga
     5101 gctcggactg gagcagcgcg cgcatcgtct cgtcgcggac cacgaccgtc gcggcgggct
     5161 tgatctgcgg cgggaccttg tggtcggcgc ggaagcgccg cagcaccgcc acgatctgct
     5221 tgaggaactc gaatcgctcg accgcgccgg cctcggcggc caaggctgcg ggccatgggc
     5281 cgcgaaggat cgtggcggtg ccggacagcg cgccgtgcag ctcctcggtc acgaacggca
     5341 tgatcggatg cagcagccgc agcgtcgcgg acagcacgtg ggtcaggatc gcgagcgtgt
     5401 cctgcttctg gtcctcgctg ccgccctgca gccgtccttt ggaccactcg acgtaccagt
     5461 cggcgaagtc gttccacgcg aagttgcgca gcgtcacggc ggccgtggcg aagtcgtagc
     5521 cttccatcgc cgcgtcggcc tcggccgtca cggccgccag acgcgccagg atccagcggt
     5581 cggccacggt ctgccgatcg gccggcggca acggccgctc ctgcggctcc ccggcgtaca
     5641 tcagcacgaa acgcgtcaag ttccacagct tgttggcgaa gttgcgatca ccctcgaccc
     5701 actgctcgct gaccggaaca tcgccgccga gcaccgcgga tcggaacaag gtcatgcgcg
     5761 tcgcgtcggc gccgtagcgc tcgatcaggt ccagcgggtc catcacgttg ccgaacgact
     5821 tcgacatctt cttgccgcgg aagtcgcgca ccatgccgtg gatgtgcacg tcacggaacg
     5881 gcacgtcgcc catcatgtcg atgccgtcca tcatcatgcg cgacacccag aacgtgatga
     5941 tgtcgtagcc ggtcacgagc acgtcgttgg gatagaaggt tcgcagctcc tcggtgtcgt
     6001 cgggccagcc cagcgtcgag aacggccaca gcgcgctcga gaaccatgtg tcgagcacgt
     6061 cctcgtcctg gcggatcgag gcgctcgcgc attcggcgca cgcatcgggg tcggttcgcg
     6121 acgcgaactg gtggccgttc tcgcagtacc acaccgggat ccggtggccc caccacagct
     6181 ggcgcgagat gcaccagtcg cgcaggttct ccatccagtc caggtagttg cgcgcgtatc
     6241 gctcggggac gtaccgggtc cggccgtcct tgaccgccgc gatcgcgggc gtggccagcg
     6301 gctcgatctt gacgaaccac tgctccgaca gccacggctc cacctcggtg ccgcagcggt
     6361 agcagtgccc caccgcgtgc gtgaaaggcc gctcctcgtg gccgaccgcg ccggccgagc
     6421 gcagcgcctc caggacgttc ttgcgcgcct cgaaccggtc ctgccccgcg aaatccgcgc
     6481 cgagctcgtt gacggtgccg tcgtcggaca ggacgttgat cgcgtcgagg ccgtgccgcc
     6541 gcccgatctc gaagtccacc gggtcgtgcg cgggcgtgac cttgagcgcg ccggttccga
     6601 actccatctc gatggcttcg tcggcgatga tcggaatctc gcggccgacc agcggcagcg
     6661 tcacggtctt gccgatcaga tccttgtagc gctcgtcgtc gggatgcacg gcaacggcgg
     6721 tgtctccgag catcgtctcg acccgggtcg tggcgatggt catgtggccg gagccgtcgc
     6781 taagcggata gcgaagctgc acgagctcgc cgtccacgtc gcggtgctcc acttcgatgt
     6841 cggagagcgc ggtatggcag cgcgggcacc agttggtgat gcggttgccg cgatagatca
     6901 ggccacgctc gtaccagcgc acgaagatct cgcgcaccgc gcgcgacagg ccctcgtcca
     6961 tggtgaagcg ctcgtaggac catgcgcacg agtcgccgag gcgccgcatc tgatgcgtga
     7021 tgcgcgagcc cgacttctcc ttccacgccc acacgcgctc gaggaacgcg tcacggccca
     7081 gatcgtgccg ggacagccct tcgcccgcga gctcgcgctc gaccacgttc tgggtggcga
     7141 tgccggcgtg atcggtcccg ggcagccaca gcacgcgaaa gccctgcatg cgcttgcgcc
     7201 gcacgatcgc atcctgaagc gagtgatcga gcgcatggcc catgtgcagc gagccggtga
     7261 cattcggtgg cggcaagacg atgcagaacg gctgcccgtc gccgtggggc tcgaacagac
     7321 ccgcggcttc ccacgtgtcg tagagccggc tctccacggt cgccggatcg tatgccttcg
     7381 gcagctcggt tcccatcgtc atctcctcgt gaggactcga aaggataaac aaaaaaagga
     7441 cccccgcagg ggtccggagg ttgcgtgagg tcacgcccgg ccgggccccg cctccgatac
     7501 gacgatcgcg ctcatgcgtc aatgctagcg agcgaggcgg ctgggggcga acggcttttc
     7561 gatcagcggc aagaggtgac ggcggttccg gtcggatccg ctccggcgca gaacgcgtcg
     7621 gcgacgatgc cgatcggacc aaaaacgagc cccatcgcga ggcgatatgc gaagagcgtg
     7681 ctctcgcgcg tcttgcggca gccgtgggtc gcggcgccac ccagatcgcc gcaggcgtgc
     7741 ggagcgagtg caccggttgc ggcgttcctg acacggtccg cgcgttcgtg gcagtccccg
     7801 cgccgaagcg ccggacagtc gatcccggtc accttcagcg tcagcacgtt cgccgtgtcg
     7861 acgagatctt ggcaatccac cgccacgtcg gcctcgtccg tgctcacgcc ccagtggcgg
     7921 cattcctcgt tgagatgcga ctctccggga agcgccagac ccgcagacgg caggaaggca
     7981 aggccggaaa gcgcgacggc gagcatgcga atccgcagcc cggccttcgt gctcccggcc
     8041 ggatgagtcg tcatgcccgc tatgcgcacg aggtctcgac cgtcccggtc gggtccacgc
     8101 cggcgcagaa gcgctcgtgg aacttgcggg cgggggcgaa cgcggcaccc atcgccagcc
     8161 gccaggcgcc gacgagatcg ctgcgcgtct cgtcgcatcg gacggtgaag ccgggatggt
     8221 cgccgcagat gtggggcgcc agcgcggcga acgcctcgcg acgggcctgt tccgcgtcct
     8281 cgtcgcattg gccgcgcgag agcaccgggc aatcaacgcc cgtgaggtcc ttcacgagaa
     8341 tgtcggcggc cctcgcggcc gctcggcagc tcgcgctcac gtgcaggtcg tcccggtcgg
     8401 cccggtactc acggcacgag tcggatatct ggctcgccgc gggcgcagcg gcccccgtcg
     8461 ccggagccac ggccagcgcg gcaagcagcg cggcgatcag cttcagatgc atgggcgcct
     8521 ccccccattc ctcactattc gggcatttcg acgccgccgc gccgggttcc ttccccgaac
     8581 gcgagacgac cccgccgaag cggggccgtt cgaagttcgg tggctaggcc gagcgttccc
     8641 ggcggatctt ggccggaggc tcggcagccc cgtccttggg ctccagacgc gggggcaggt
     8701 gctcgaccac cgattcacgg ctgatcacgc acttgccgac gtcctcgcgg ctcggcaggt
     8761 cgtacatgac gcccagcagg acctcttcca tgatcgcccg caggccgcgc gcgccggtgc
     8821 cacgcttcat ggcctcgtcg gcgacggcgt ccagggcgtc ttcgtcgaag ctcaggtcga
     8881 cgccgtcgaa ctcgaagaac ttgcggtact gcttcaccag cgcgttcttc ggctcggtca
     8941 ggatcttgac gagcgcctcg cggtcgaggt tgttgaccgc cgtgatcacc ggcagacggc
     9001 cgatgaactc ggggatcagg ccgtaacgca acaggtcctc cggcatgacc ttgcccagga
     9061 tgttgcccac gttcttctcg tccgcacgac ggatgtcggc gccgaagccc acgcccttgc
     9121 gcccggtgcg gctttcgatg accttctcga gcccggcgaa agcgccaccg cagatgaaca
     9181 ggatgttcgt ggtgtcgatc tggatgaact cctggtgggg gtgcttgcgg ccgccctgcg
     9241 gcggaaccga cgcggtcgtg ccttcgagga tcttcagcag cgcctgctgc acgccttcgc
     9301 ccgacacgtc gcgagtgatg gacgggttct cgctcttgcg agcgatcttg tccacctcgt
     9361 cgatatagat gatgccggtc tcggccttct tgacgtcgta gtcggcggcc tggatcagct
     9421 tcagcaagat gttctcgacg tcctcgccga cataaccggc ttcggtcagc gctgtcgcgt
     9481 ccgcgatcgc gaagggaacg ttgagcatct tcgcgagcgt ctgcgccagc aacgtcttgc
     9541 cgcagccggt cggaccgagc agcaagatgt tcgacttctg gagctcgacc tcgccgtcgt
     9601 ggtacgcccc gacctggatg cgcttgtagt ggttgtagac cgcgaccgaa aggaccttct
     9661 tggcttcgtc ctgaccgatg acgtagtcgt tgaggaattc gtagatctct ttcggcttgg
     9721 ggagctcctc gagcttgagc tcggtggact cgttgagctc ttcttcgatg atctcgttgc
     9781 acaggtcgat gcactcgtcg cagatgtaca cgcctggccc tgcgatgagc ttcttaacct
     9841 gcttttgcga cttgccgcag aaagagcact tcagcagatc gccgccgtca ccgaatttcg
     9901 ccacccggac tcccctctcg gcgcttagct cgcggccgcg gtcttttcca gcttgtcctg
     9961 cgcgtgacgg ctggaaataa cctcgtcgat tataccgtag gccttggcct cttccgcggt
    10021 catgatgaag tcgcggtcgg tgtctttgga gatcttctcg agatcctggc cggtgtgctc
    10081 ggcgaggatc tggtcgagga gccggcgata gcgcagaatc tccttcgcct ggatctcgat
    10141 gtccactgcc tgaccctgcg ccccaccgtg cggctggtga atcaggatgc gtgcgtgggg
    10201 caacgcgtag cgcttgcccg acgcgcccga cgcgagcagc acggccgcgg ccgatgccgc
    10261 ctggcccatg cagatcgtcg aggtgtgcgg cttgatgtac ttcatggtgt cgtagatcgc
    10321 gaacagcgca gtgatcgagc cgcccggcga gttgatgtag aggtggatgt ccttgtcggg
    10381 atcctccgac tcgaggtgga gcatctgcgc catgatcagg ttcgcgaccg cgtcgtcgac
    10441 ctcggtgccg agaaagacga tgcgctcctt gagcaggcgg ctgtagatgt cgaacgcgcg
    10501 ctcgccgcgg ttcgtcgact cgatgacggt gggaaccaga tagttctgca tccgaaagtc
    10561 tccttcgccg atccggcgcg ccgcccctgg cggcctgccc cggaccttcg tccttatgaa
    10621 cgacgcggac ccgcctcggc tactcggcgt ccgcgttctc ttctttgatg tcggctttct
    10681 ccacgaccaa gtcgagagcc tttctcctga gaatatcacc ggccaccacc ccgacccggc
    10741 cggcctgttc gagacgtttg cgcagttcag gaccattttc gcccgacgcc tcggcgagac
    10801 ggccgatctc ctcgtcgagc tcggcctctt cgaccgtgag ctcctcccgg cgtgcgatct
    10861 cttccaggat caactgagcc gacaccgagc gctccgcttg cttgcggata tcggcctcga
    10921 tctcctgctc ggtcgcctcg gccgacgtca gatagtcgtc gagcgagaca cccgcaccgc
    10981 gaagctgatc ggagagtcgg tgaagccggt aggccacctc ctcgtcgacc atggcgtcgg
    11041 gcgcggcgac cggcaccgcg ttgaccagca cgtcgagcat cctggacctg acctcggcat
    11101 cggcgccgga gcgcttgatc cgggtgatct tgtcggccag gtcggccttg agctcgccga
    11161 gcgtctcgaa ctccgaggcc gtcttggcga actcgtcgtc gagcgccgga gtgttcttct
    11221 gccgcacctc tttgaccagc acctggaagg tgacctcgcg tccgccccag gtggagccga
    11281 acgtgggcgg aagctctgcg ttgaacttga ggatgtcgcc ggctttcttg cccagcacct
    11341 ccgcgtcgag ctcgggaacg atccggccgc tgcccgcctc gtagagcatg tcgttggcgg
    11401 tggcgttgtc gatctcctcg tcgttccagt agcccttgat gtcgatcagg acgaagtcgc
    11461 cctcggtcgc gggccgctcg acggtctcga gcgaggcgaa ccggtcggcg aggcgctcga
    11521 gctggttctg cacgtcttcg tcggtcgcgg cgaacggcgg ccgcgtgacc ttcaggccgt
    11581 tgatgtcggg cagatcgatc tccggccgga cctcgacgat ggcctcgaaa atcagcggct
    11641 cgtccacctc gaaggaggtc acctctatct gaggaggggc gaccgggtcg atgtcctcgc
    11701 cgaccacggc ctgcgcgtag aactgcggca cggcctcgcg gacgatcatc tccttgactt
    11761 cgtccttgcc gaggcgggcc tcgagcaagg agcgcggcgc cttgccgggg cggaagccgg
    11821 gaatcttgac ttgcttggcg agctgcttga acgctcggtc ggcggcaggc ttgatctcgt
    11881 ccggggcgac ctcgacggtc atccggatac gagtggggcc ctcgcgggaa acggtggtct
    11941 gcatgggagg cgcatcatac gccggggggc cggggccgtg cggaagaatg cggcgagtcg
    12001 tccgcgtccc gcggcccgac tcgtcgggga gagaggattc gaacctccgg cctccgcgtc
    12061 ccaaacgcgg cgctctggac ctggctgagc tactccccga gtgcgccgtg cagtgtagcc
    12121 gcccggcaac gctacaatgg cgccgccgtc cgcgggtgta gctcaatggt agagctccag
    12181 ccttccaagc tggctatgag ggttcgattc ccttcacccg ctcagagaca cacacaccga
    12241 ttcgccccgg gattcgttcg ctcctactgg tagaatcggt ggtcgcgcga ccgcacgaca
    12301 gacccgcgcc cgtagctcag gtggatagag cggctgcctt ctaagcagcg ggtcgggggt
    12361 tcgagtcctc ccgggcgcac gacgagcact acgaacatgg tggccgtagc tcagctggtt
    12421 agagcgccgg gttgtggtcc cggaggtcgg gggttcaagt cccctcggtc accccagcgc
    12481 cggtccgcag cgccggtccg cttcaccgcc gcgtcgaatg atcggtggaa aagaggtatt
    12541 gtttcgcccc gcgtgccgtg gatccaccct cggtcgcgaa tacgggccgc tagctcaact
    12601 ggctagagca tctgactctt aatcagaggg ttcggggttc gagtccctgg cggcccactc
    12661 caaggggatg cgttctcgca tcccctttct tttcccctga tcccggttcc gtccgctcgc
    12721 ttgctgctgc aagcgcctga cggtgcatcc gtgtccgccg ttacagatgc ggaacaccca
    12781 cgaaccgtag ggtctctgat gaaccggaag aaccgacgaa attctgtttc ttccaagtct
    12841 tccctcgggc aaggtcgttg atgagggagg tgcccaggaa gggcgctatc cggcagaagc
    12901 ggatggcgca ttccgaaagc ggccgctact gtgacagggg cggcaagggg gacatgggga
    12961 aatgttgaag gccgggaaaa ccacgagcaa gacgaagtat gtcgtcatgg ccgggatcgt
    13021 ggccgctgcg ggagcgtttt cgatcctgat ggcggtccgg ccggccaacg cccagcagct
    13081 ggccacgttg aaggtcctga gcgggtccgt gtccgtctcc gttcaaggag ccgccgttgc
    13141 cgccgcgacc gacgggatgt ccgtgaaggc cggcgacgtc gtcgagaccg gtgaccccgg
    13201 ccgcgccgtc gtgcgctact tcgacgggag cgagacgcgc ctggactcga acacccgctt
    13261 cgaggtccgc gagttgcaca cgctgcccaa caaagcccaa agcaaggtca tccgaggaaa
    13321 gcagacgaag ggtgcaacct tcaaccgcat cgtgaagctg accgacagcc agagccgggt
    13381 ccagaccgaa gcgccgacat cgacggcttc cgttcgcgga acggcctact ccgggacctc
    13441 gaacgccgac ggaaccttca cgtggcgagt cttcgaaggc gcgatcaagg tcaccgactc
    13501 gttcggcaag tccgtgatcg tcaaggccgg ccagggcgtc atcgtcgatg aggacggcag
    13561 gctgctcgag gtctacgacc tcagcgacga agagcttcag gccgagtggg tcacgttcaa
    13621 caactgcaag cttgacggta tgggctgccc gcaggtcctc gacgacaagc agacgggcgg
    13681 ccccggcacg cccaccgacg agccgcaggg tccgcagggt ccgcag
//
LOCUS       DATLFO010000368         9179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_9193, whole genome shotgun sequence.
ACCESSION   DATLFO010000368 DATLFO010000000
VERSION     DATLFO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9193"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VM841_15105"
     CDS             <1..421
                     /locus_tag="VM841_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91548.1"
                     /translation="AGRITGRGRVSRLTVMRPPAGDAVTLPAPGDILWMEGDVVREGN
                     ESSVVLHALVALPDGSVAGGRVAEATADSFEITIEESPLLPPRVVDPGTGGAFVDLSA
                     GEERRGPDGRATSGVDEVVEDSFPASDPPSFAGVPSD"
     gene            complement(448..723)
                     /locus_tag="VM841_15110"
     CDS             complement(448..723)
                     /locus_tag="VM841_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020441857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVL91549.1"
                     /translation="MIVESIHDWQTQAACRGMDPAIFFSPDDDAAGVARAVCSICPVK
                     AECLSEAMSRGEVYGVWGGTRERDRRADPPRRARPDRIATTTAAAQA"
     gene            945..1379
                     /locus_tag="VM841_15115"
     CDS             945..1379
                     /locus_tag="VM841_15115"
                     /inference="COORDINATES: protein motif:HMM:NF016564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsgA family sporulation/cell division regulator"
                     /protein_id="HVL91550.1"
                     /translation="MNDFDPGFTEIRDLDTQATLRVSLFGVGPAPTALLFATYRQVDP
                     LIITLEVRTPEEVTVFRRLLLRADFAVGLTGPTAHAGTRMLPVEGRMIEISFTEDGAS
                     VAVRIPEAAARRFLGLSYRIVPIDDEGAVLGDLAAELALDRG"
     gene            1577..2782
                     /locus_tag="VM841_15120"
     CDS             1577..2782
                     /locus_tag="VM841_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HVL91551.1"
                     /translation="MPATERSITTADAIEHIRSHALSTDDDGRVGAEVEWVVVDPSDR
                     LARTPVATVASLASSAARTRVTFEPGGQLELSSDPGGLTAVCDALSSDEEVVARRLQS
                     AGLTMIGIGLDPIRADRRVVFGPRYDAMERYFDADGPEGRRMMCGTASIQVNVDAGGP
                     AVRDRRWRRAHTIGPMLVAAFANSPFAAGAPTGWRSTRLANWWAMDRSRTAPPPMSDD
                     AAGTWASYALAANVMMIGDGERFDAPEIRFPFRDWIERGYAGLYPTIADLDYHVTTLF
                     PPVRARGWLEIRYLDAQEAPWWRAAVAVTVALMNDAQAAGVAEQVCAPLAGRWMEAAR
                     DGLSSPGFAAAARACFDAVTAALPRLEADDVTAEAVRRFVDTFVARGRCPADALMERP
                     ATSPALVAR"
     gene            2779..4116
                     /gene="egtB"
                     /locus_tag="VM841_15125"
     CDS             2779..4116
                     /gene="egtB"
                     /locus_tag="VM841_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HVL91552.1"
                     /translation="MTETMSFNARPSQAALKERAAAELTVVRGRSLGLIDPLPEHEQV
                     RQHSSIMSPLIWDLAHVGNYEDIWLLRAVGRESVDSRYDDMYNAALHPRSERPQLPLL
                     GPGDARRYIAEVRGRVLGALEDVELREEDPLLRDAFVYGMVIQHEHQHDETILATLQL
                     MEGDGYARYAAIATPPPVSAPPLLAEEVYFSGGPVVIGTDDEPWAYDNERGAHTVDLA
                     PFFIDATPVTNGQFLRFVEDGGYHEPRYWDERGWRARVKGGFEHPMFWQRDGGGWSVV
                     RFGRREFLRDGEPVQHVSWYEADAYARWCGKRLPTEPEWEAAASWDPAAGRKYRYPWG
                     DREPDGAVANLGQRHFGPATAGALPEGASPIGARQMIGDVWEWTASDFLPYPGFVAFP
                     YPEYSEVFFGPRYKVLRGGSWATHPAAIRATFRNWDLPVRRQIFAGFRCARDA"
     gene            4117..4893
                     /gene="egtC"
                     /locus_tag="VM841_15130"
     CDS             4117..4893
                     /gene="egtC"
                     /locus_tag="VM841_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HVL91553.1"
                     /translation="MCRMLCYLGPPVTLAELLLDPPQGLAEQAWKPRYQTHGAMNADG
                     FGAGWYDPARSDTPALYRRAASIWSDRTFASIARLVASSAVLAAVRSATPPLPVHEFN
                     TPPYTAGPWLFAHNGAIDGFAGEAGVRLRAMVTPRRAAAIEGSTDSEVIFALTLDALD
                     AGVEPPDAIRQVVERIDAVASARLTMLMTDGVHAYAIARADSLFVRQGGCVTIASEPF
                     GGDEGWERVPDGSLVEASSAGVRRESLDHRASSATAKEPA"
     gene            4890..5873
                     /gene="egtD"
                     /locus_tag="VM841_15135"
     CDS             4890..5873
                     /gene="egtD"
                     /locus_tag="VM841_15135"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HVL91554.1"
                     /translation="MKASSTVSLDVRLTDEDLRAGLAADVAAGLAGDPKTLPSKWLYD
                     EAGCELFDRITTLPEYYPTRRERSILRDRAREIARITAAETLIEIGSGTSEKTRLLLD
                     ALAGSGTLSRIVLLDISEPTLRAAADTLADAYPGVDVHAVVGDFTRHLDAIPAGDRRL
                     IAFLGGTIGNLTPAQRSSFFAGLSRDASDGDALLLGTDLVKDPGRLVAAYDDAAGITA
                     AFNLNMLRMINRELGGDFDLGAFEHVAVFDEAEEWIEMRVRSLRDQTVTVTGLSERYR
                     FAAGELMRTEISAKFRKAGVTAELAAGGWMLDRWWTDERSDFALSLSRPAR"
     gene            5983..6300
                     /locus_tag="VM841_15140"
     CDS             5983..6300
                     /locus_tag="VM841_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVL91555.1"
                     /translation="MRVTVRRGPYGCPVVEPVGEVDFSTRDQLAEQLSLALSVGKPLL
                     FLDLRGLTFIDSAGVHVIRRTLIRMVQGRILAVRAPAHIQRIVEFLGLGDDLDFLDVL
                     PGE"
     gene            6398..7279
                     /locus_tag="VM841_15145"
     CDS             6398..7279
                     /locus_tag="VM841_15145"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HVL91556.1"
                     /translation="MPSSLTPGSLRQGDHACSWYSTDEHRDSVAAPFVSAGLLANERV
                     LYLHTDSSEDSVMEMIRRCGTDTEAALADGSLALRPAADVYVPGGGSFDPGRTVERLE
                     DEVADARRAGYQGLRIAAEMSWAIEGSGGGDLADYEHRANDVFDRAALTAICLYDRRR
                     FPAGGPSPGAAHNLHVWPDAPSYSRMLSVSITHTPRPDGLKLEGEVDFATSIVLAEAL
                     TRVVDTAAGDVHIDLSGLRFIDIGAARLLARAARQLGSGRALVLDAPSRAVRRLLKVL
                     DGRLTAGFVVTEDGVES"
     gene            7276..7758
                     /locus_tag="VM841_15150"
     CDS             7276..7758
                     /locus_tag="VM841_15150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91557.1"
                     /translation="MSSTMYPRDPARPEMEEMETPQGSGRLPRTHGILASIRIDGTGA
                     LRDLRQAVLDTAAAAQLTEQRVLDMALAANEIATNAILHGGGAASVQIWIDERGWHCD
                     VIDDGPGLSDPTAGTRPPGVQEGGFGMWIARRLCDEFEIVPRQSGLHVRLVAHRGHAR
                     "
     gene            complement(7755..8240)
                     /locus_tag="VM841_15155"
     CDS             complement(7755..8240)
                     /locus_tag="VM841_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010836244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HVL91558.1"
                     /translation="MEIVFRAAVTFAFLAVLMRAMGRRELSEITTFELVLLVTMGDLV
                     QQGVTQEDMSLTGAMLAVGTIALLILATSYVSFRYPKTRRVLEGMAVVVVRDGRILDE
                     VTRLERLTPEEIMEGARQRGIADLRRVKVGILEADGRFSFITGDGSRPDGEEPPPRRA
                     S"
     gene            complement(8240..8770)
                     /locus_tag="VM841_15160"
     CDS             complement(8240..8770)
                     /locus_tag="VM841_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91559.1"
                     /translation="MSERIDDVAAGTATDDVREIRRARSGRRAWLAGLTVLVLAGASG
                     LLGIRTAEATSEAGPYELTVTYPSIARPGIAISWEVKVVRAGGFTGPIRIATASDYLA
                     MFDENGLDPDPASATADGTELIWEFDPPPGDTLVVSYDARLEPSVQMGRRGTTRVLDE
                     AGTTIVAVSYRTWVMP"
BASE COUNT         1345 a         3135 c         3192 g         1507 t
ORIGIN      
        1 ggcgggacgc atcaccggcc gcggacgtgt atcccggctg acggtgatgc gtccaccggc
       61 aggcgacgcg gtgacgcttc cggcgcccgg cgacattctg tggatggaag gcgatgtcgt
      121 ccgggagggc aacgagagct cggtcgttct gcacgcgctg gtcgcacttc ccgacgggag
      181 cgtcgcgggt ggcagggtgg ccgaggcgac cgccgactcg ttcgagatca ccatcgagga
      241 gtccccgttg cttcccccgc gggtcgtgga tccgggcacc ggcggtgcct tcgtggacct
      301 gtcagcgggc gaggagcgac gcggacccga tggccgggcc acttctggag tggacgaggt
      361 cgtcgaggat tcgtttcccg cgagcgaccc cccgagcttt gccggcgttc cgtcggactg
      421 aaggggccgc gggcgcgaac gggccggtca ggcctgtgcg gcggcggtcg tggtcgcgat
      481 gcggtccggg cgagcccgcc ggggcgggtc agcgcgccgg tctcgttcgc gcgtccctcc
      541 ccaaactccg tagacctcgc cccggctcat ggcctcggag aggcactcgg ccttgacggg
      601 gcagatgctg cagaccgcgc gggcgactcc ggccgcgtcg tcgtcggggc tgaagaagat
      661 ggccgggtcc atgccgcggc aggccgcctg cgtctgccag tcgtgaatgg attcgacgat
      721 cattcgtctg cctcggtgtt gccgtcttcc cgatcgtctg cgggtacgac agggcgggcc
      781 tcggaggcga acgcgtagca ctgcagcatg gaccgaaggt cggagcgggt catcggaccc
      841 ttgattccca atattgggca acccaaacct gagcgaagca gtcgcgtatc gcggattctt
      901 cttcgggttt ttccgggcgt cttcctggga actcaatcgg tgagatgaac gatttcgacc
      961 ccggtttcac agagatcagg gacctcgata cgcaagccac gttgcgtgtg tccttgttcg
     1021 gagttggccc ggctcccacg gcgcttctgt tcgctaccta tcggcaagtc gatccgctca
     1081 tcatcacgct cgaggtccgg acgccggagg aggtcaccgt ctttcgccgg cttctgctgc
     1141 gcgcggactt tgcggtcggg ctcaccggcc cgaccgcgca cgccggaacg cgcatgctcc
     1201 ccgtagaggg ccgcatgatc gagatctcct tcaccgaaga tggcgcatcg gtcgctgtgc
     1261 ggatcccgga agccgcggcg cgtcgcttct tggggctcag ctaccgaatc gtgccgatcg
     1321 acgatgaagg agccgtgctc ggggatcttg cggccgagct ggcgctggac cgcggatagc
     1381 ccgcggccgc gggcgctgaa acgaatccgg ctctcgcgag acggaatccg ggtggaagga
     1441 gccaagaacc ctggatgagc ggaatcgagc gagggcggct ggattcatgg ccgttggcgg
     1501 gcgacggccc cggcgggtcg gctacgctgt cagccgagca agcgccgccg acgggaccgg
     1561 gggcgagggg cggctgatgc cggcaacaga gcgatcgatc accaccgcgg acgcgatcga
     1621 gcacatccga tcgcacgccc tgtcgaccga cgacgacggc cgtgtcggcg ccgaggtcga
     1681 gtgggttgtc gtggacccgt cggatcgcct ggcgcgaact ccggtcgcca ccgtcgcgtc
     1741 gctcgcctcc tccgccgcgc ggacgcgcgt caccttcgag cccggaggcc agctcgagct
     1801 cagctcggac cccggcggcc tgactgcggt ctgcgatgcg ttgtcctcgg acgaggaggt
     1861 ggtcgcccgt cgtctgcagt cggccggctt gacgatgatc ggcatcggcc tggatccgat
     1921 ccgcgccgac cggcgcgtgg tcttcggacc gcgctacgac gcgatggaac gctacttcga
     1981 cgccgacggc ccagagggcc ggcggatgat gtgcggcacc gcctccattc aggtcaacgt
     2041 agacgcgggc ggcccggccg ttcgtgatcg ccgctggcgg cgcgcccaca cgatcggccc
     2101 gatgctcgtt gcggctttcg ccaactcgcc gttcgccgcg ggcgcgccga cgggctggcg
     2161 atcgacgcgg cttgcaaact ggtgggcgat ggaccggtca cgcaccgcac ccccgccgat
     2221 gagcgacgac gccgccggta cctgggcgtc gtatgccctg gcggccaacg tgatgatgat
     2281 cggcgacggg gaacgcttcg acgcacccga gattcgcttc ccgttccgtg actggatcga
     2341 gcgcggctat gcgggtctgt acccgacgat cgccgatctc gactatcacg tgacgacgct
     2401 gttcccgccc gttcgcgcgc gcggctggct cgaaatccgt tacctggacg cgcaggaggc
     2461 tccctggtgg cgcgccgcgg ttgccgtgac cgtcgcgctc atgaacgacg cgcaggcggc
     2521 cggcgtcgcc gagcaggtct gcgcgccgct ggcgggacga tggatggaag ccgcgcgtga
     2581 cgggctgtcg agccccggct tcgccgccgc ggctcgtgcc tgcttcgacg cggtcaccgc
     2641 cgctttgccc cggctcgagg ccgatgacgt caccgccgag gcggtccgcc gcttcgtcga
     2701 caccttcgtc gcgcgagggc gctgccccgc cgatgcgctc atggagcggc cggcgacgtc
     2761 gccggcgctg gtcgcccgat gaccgagact atgtccttca acgcgcggcc gtcccaggcc
     2821 gcgctcaagg agcgagcggc cgcggagctg acggtggtcc gcggccgctc gctcggactg
     2881 atcgatccgc tccccgagca cgagcaggtc cgccagcact cgtcgatcat gtcgcccctg
     2941 atctgggatc tcgcgcacgt cggcaactac gaggacatct ggctgctgcg ggcagtcggc
     3001 agggagtccg tggactcgcg ctacgacgac atgtacaacg cagcgctgca tccgcgctcc
     3061 gaacgtccgc agctgccgct gctcggtccc ggcgatgccc gccggtacat cgccgaggtc
     3121 cgtggccggg tgctcggagc cctcgaggac gtcgagcttc gcgaggagga tcccttgctc
     3181 cgcgacgcct tcgtctacgg catggtgatc cagcacgagc atcagcatga cgagacgatc
     3241 ctggccacgt tgcagctcat ggaaggcgac ggctacgcgc gctacgcggc catcgcgacc
     3301 ccgcccccgg tgtcggcgcc gccgctgctc gccgaggagg tctacttttc cggcggcccc
     3361 gtcgtcatcg gaaccgacga cgagccgtgg gcctacgaca acgaacgcgg cgcgcacacg
     3421 gtggatctcg ctccgttctt catcgacgcc acgccggtga ccaacggtca gttcctgcgg
     3481 ttcgtcgaag acggcggtta tcacgaaccc cgctattggg acgagcgagg ctggcgcgcg
     3541 cgcgtgaaag gcggcttcga gcacccgatg ttctggcagc gcgatggcgg cggctggtcg
     3601 gtcgtccgct tcgggcggcg cgaattcctc cgcgacggcg agccggtcca gcacgtgtcg
     3661 tggtacgagg ccgatgccta cgctcgctgg tgcggcaagc ggcttccgac cgagcccgag
     3721 tgggaggcag cggcgtcgtg ggatcccgct gcgggccgca aataccgcta tccctggggc
     3781 gaccgcgagc cagacggcgc cgtcgccaac ctcggccagc gacacttcgg tcccgcgacc
     3841 gcgggtgccc tgccggaggg ggccagcccg atcggggccc ggcagatgat cggtgacgtg
     3901 tgggagtgga cggcctcgga cttccttccc tatcccggct tcgtggcctt cccgtatccg
     3961 gaatactcgg aggtcttctt cggtccgcgc tacaaagtgc tgcggggcgg ctcgtgggcg
     4021 acgcatccag ccgcgatccg agccacgttc cgcaactggg acctgccggt ccggcggcag
     4081 atcttcgccg gcttccgctg cgcgagggac gcttagatgt gccggatgct gtgctacctc
     4141 ggcccgccgg tgacgctcgc cgagcttctt ctcgatccgc cgcagggtct tgccgagcag
     4201 gcgtggaagc cgcgctatca gacgcacggc gcaatgaacg ccgacggctt cggcgccggc
     4261 tggtacgacc cggcccgcag cgacacgccc gcgctgtacc gcagggccgc atcgatctgg
     4321 tccgaccgga ccttcgcgag catcgcccgg ctcgtggcgt cgagtgcggt cttggcggcg
     4381 gtccgcagcg cgaccccgcc gcttccggtg cacgagttca atacgccgcc gtacaccgcc
     4441 ggcccctggc tgttcgctca taacggcgcc atcgacggct tcgccggcga agccggggtg
     4501 cgtctgcgcg caatggtgac accgcgccgc gcggccgcga tcgaaggttc caccgactcc
     4561 gaggtgatct tcgcgctgac gctggatgcc ctcgatgccg gagtggagcc cccggacgcg
     4621 attcgacagg tcgtcgagcg aatcgacgcg gtcgcatccg cgcgtctgac catgctgatg
     4681 accgacggcg tgcacgcgta cgcgatcgcg cgcgcggatt cgctattcgt gcgccagggc
     4741 ggctgtgtca cgatcgcgtc ggagccgttc ggcggcgacg agggctggga acgcgtgccc
     4801 gacggctcgc ttgtcgaggc aagctcggcc ggcgtccggc gcgagtccct cgaccaccgt
     4861 gcttcgtccg ccaccgcaaa ggagcccgca tgaaagcctc atccaccgta tcgctcgacg
     4921 ttcgcttgac cgatgaggat cttcgggccg gtttggcggc cgatgttgca gcgggcctgg
     4981 cgggcgatcc caagacgctt ccttcgaagt ggctctacga cgaagcgggg tgcgagctgt
     5041 ttgatcgcat caccactctt cccgagtact acccgacgcg acgggagcgc tcgatcctgc
     5101 gggaccgggc gcgcgagatc gcgcggatca ccgcggcgga gaccttgatc gaaatcggct
     5161 ccggcacgtc cgagaagacg cggctgctgc tcgatgccct cgccgggagc ggcacgctat
     5221 cgcgaatcgt gctgctcgac atcagcgagc cgacgcttcg cgcggcggcc gatacgctcg
     5281 cggacgccta tcccggcgtt gacgtgcacg cggtggtcgg cgacttcacc cgtcacctcg
     5341 acgccatccc cgcaggggac cgccggctca tcgccttcct gggcggaacg atcggaaatc
     5401 tcacgcctgc acagcgctcg tcattcttcg ccgggctgtc gcgggacgcg tcggacggcg
     5461 acgcgttgct gctgggaacc gacctcgtga aggatccggg gcggctcgtt gccgcctacg
     5521 acgacgcggc cggcatcacg gcggcgttca acctcaacat gcttcgcatg atcaaccgcg
     5581 agctcggcgg ggacttcgat ctcggcgcct tcgagcatgt cgcggtcttc gacgaggccg
     5641 aggagtggat cgagatgcgc gtccggtcgc tgcgcgacca gaccgtcacc gtaacaggcc
     5701 tgagcgagcg ctaccgcttc gccgccggcg agctgatgcg gaccgagatc agcgcgaagt
     5761 ttcgaaaggc aggcgtgacc gcggagcttg ccgccggcgg ctggatgctc gaccggtggt
     5821 ggaccgacga gcgaagcgac ttcgctctgt ccctttcccg gcccgcgcga taaatctccg
     5881 cccaggaggc ataggacgtg ttgcatccgg gtacaaccaa gcagaagcga gcgccggagg
     5941 tggaaggtgg agtccgcagt ccgcgtagca agggcagacc gcttgcgcgt caccgtgcgc
     6001 cgcggaccgt acggctgccc ggtggtcgag ccggtcggtg aggtcgactt ctcgacccga
     6061 gaccagctcg cggagcagtt atcgctcgcc ttgagcgtcg gcaagcccct tctgttcctc
     6121 gacttgcgag gacttacctt catcgattcg gccggtgtcc atgtgatccg ccggacgctg
     6181 atccggatgg tgcaaggacg gatccttgcc gtccgagcgc ccgcccacat ccagcgcatc
     6241 gtcgagttcc tgggcctcgg cgacgatctg gacttcctcg acgtgctccc cggcgagtaa
     6301 gcaagcagtg gccgtcccgc cgtcccgcgg cgggggtttc gtgcgtgccc atttcgggga
     6361 aaacacaccc attcgaaagc cggatcgagg agttgtaatg cccagtagtc tcacgcctgg
     6421 ctctctccga caaggggacc atgcgtgctc gtggtactcg acggatgagc accgggattc
     6481 cgtggcggcg ccgttcgtct cggcgggcct gctcgccaac gaacgggtcc tgtacctgca
     6541 caccgattcg tccgaggact cggtcatgga gatgatccgc cggtgcggga ccgacaccga
     6601 ggccgctctc gccgacggaa gcctggccct tcgccctgcg gcggacgtct atgttccggg
     6661 cggcggctcc ttcgaccccg gccgcacggt cgagcggctc gaggacgagg tggcggacgc
     6721 ccggcgagcg ggctatcagg gccttcggat cgccgccgag atgtcctggg cgatcgaggg
     6781 atccggtggc ggggacttgg ccgattacga gcatcgcgcc aacgacgtgt tcgaccgggc
     6841 ggctctgacg gcgatctgcc tctacgaccg ccgacggttc ccggccggcg ggccgtcccc
     6901 gggtgccgcg cacaatcttc acgtgtggcc agacgcgccc tcgtactcac gcatgctttc
     6961 cgtatccatt acgcacacgc cgcgccccga cgggctcaag ctcgagggtg aggtcgattt
     7021 cgcgacctcg atcgtgctcg cagaggcctt gacacgcgtg gtggatactg ctgccggcga
     7081 tgtgcatatc gacctgtcgg gcctgcggtt catcgacatc ggggcggcgc gcctcctggc
     7141 gcgcgcggcc cggcagctcg gctcagggcg cgcgctcgtg ctcgacgcgc cgtcgagggc
     7201 cgtgcggcgc ctcctcaagg ttctcgatgg gaggctgacc gcaggcttcg ttgtgaccga
     7261 ggatggtgtc gagtcgtgag ctcgacgatg tacccgcggg acccggcccg ccccgagatg
     7321 gaggagatgg aaactcccca agggtccggc cggctccctc gtactcacgg catcctcgcc
     7381 tcgatccgta tcgacgggac gggtgcgctg cgagacctgc gccaggcggt cctcgacacg
     7441 gccgcggcgg ctcagctgac ggagcagcgg gtgttggaca tggcgctggc cgccaatgag
     7501 atcgccacca acgcgatcct gcacggtggt ggagcggcga gcgtgcagat ctggatcgac
     7561 gagcgaggct ggcactgcga cgtgatcgac gacgggcccg gcttgagcga tcccacggcc
     7621 ggcacccggc ctccgggtgt gcaagagggt ggcttcggga tgtggatcgc gcgacggctc
     7681 tgcgacgagt tcgagattgt tccccgtcag agcgggctgc acgtgcgtct cgtggcacac
     7741 cgcggccacg cccgctagct cgctcggcgt ggaggcggct cctcgccgtc gggccggctc
     7801 ccgtccccgg tgatgaacga gaagcgaccg tcggcttcca gaattccgac tttgacgcgc
     7861 cgcaggtctg cgatcccccg ctggcgcgct ccttccatga tctcctccgg cgtgagtctc
     7921 tccaagcgtg tgacctcgtc cagaatccgt ccgtcgcgga cgacgacgac ggccatccct
     7981 tccagcacgc gccgggtctt ggggtatcgg aacgagacgt aggaggtcgc gaggatcaac
     8041 agcgcgatgg tgcccacggc cagcatcgct ccggtcaggg acatgtcctc ctgcgtgacg
     8101 ccctgctgga cgagatcgcc catcgtcacc aaaagcacca gctcgaacgt ggtgatctcc
     8161 gacagctccc tccggcccat ggctcgcatg agcacggcga ggaatgcgaa ggtaaccgcg
     8221 gcgcggaaga cgatctccat cagggcatca cccacgttcg gtaggagacc gcgacgatcg
     8281 tcgttcctgc ttcgtccagc acgcgggtcg ttccccggcg ccccatctgc accgagggct
     8341 cgaggcgggc gtcgtaggag acgacgagcg tgtcacccgg cggggggtcg aactcccaga
     8401 tgagctcggt gccgtcggcg gtcgccgatg cgggatccgg gtcgagcccg ttctcgtcga
     8461 acatcgccag gtaatccgag gccgtcgcga tgcggatcgg cccggtgaag ccgcccgctc
     8521 gcacgacttt cacctcccag ctgatggcta tgcccggccg cgcgatcgag ggataggtca
     8581 cggtgagctc gtacggcccg gcctcgctcg tcgcctcggc cgtacggatg cccagcagcc
     8641 cggacgcccc ggcgagcacg agcaccgtaa gcccggcaag ccatgcgcgc cggccgctgc
     8701 gtgcgcgccg gatctcgcgg acatcatcgg tggcggtgcc ggccgcgaca tcgtcaatcc
     8761 gctcgctcat tgctgttttc attcccggca acgggggcac ggaaaccaag caaaaacccc
     8821 ggttgatcgt gataaatcgg tcggcaagca cagggccgat ggacagggga ggaccggccg
     8881 ggcgagaatc gatgcatcgc cgtgggggcc tgtcgaatcc tcgcggctcg tccccgtgca
     8941 gaagtttcga agctcggccc cgtccggccg gtccggcgcc cccgtgcagg gagccgatcc
     9001 ggccctgttg gcgtctgccc tcgttgatgc gtccatggac gctgtcgtgg ccgtggacga
     9061 gcgcgggacc atcgttcttt tcaacgagac cgccgagcgc ttgaccgggt ttcgtggggc
     9121 cgacgttatc gggcggcccg tgcgggagac cctcatccct ccgagcgagc gaagtgctt
//
LOCUS       DATLFO010000369         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18844, whole genome shotgun sequence.
ACCESSION   DATLFO010000369 DATLFO010000000
VERSION     DATLFO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18844"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..778
                     /locus_tag="VM841_15165"
     CDS             173..778
                     /locus_tag="VM841_15165"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HVL91560.1"
                     /translation="MSGARILVIDNYDSFTYNLVQELAELGARPTVVRNDVWTLGQVR
                     DFAADGIVISPGPGTPAKPGDVGISNDVIREMGPSTPVLGVCLGNQCIGYVYGGRIVR
                     APQLMHGKTSPIYHREEGILEGIPIPFEATRYHSLIVERASLPAELKVTAETSDEIIM
                     GLRHREHPVEGVQFHPESVMTGDGMDMLRNWLARCGAPAST"
     gene            complement(768..1097)
                     /locus_tag="VM841_15170"
     CDS             complement(768..1097)
                     /locus_tag="VM841_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91561.1"
                     /translation="MTDSTICPARAPWRILLGAALFVGAFFTVSAALSLPRAFEGSTA
                     GQNLGRLAALGYAWAVSAALIGLGAINIFSRLRGERRVLAASLVALIVGTPTVLIVWA
                     VNCLLRC"
     gene            complement(1177..>2351)
                     /locus_tag="VM841_15175"
     CDS             complement(1177..>2351)
                     /locus_tag="VM841_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HVL91562.1"
                     /translation="YFSPEQAQAAPVDARSDLYSLGVVLYEMLTRQVPFTGASPVAIA
                     YKHVKEDPIPPSRLNADVTPEIEAIVMKALSKNPDNRYQSAQEMRQDLERAIRGQAVS
                     APRVMGETAMMTAPGTADETVILRGPVAPERRKRGTGIALLAAIAAAMIGIIIWALLA
                     LLPKSPSTVPVPSVEGFTVEAAQGRLELAGFKTKVGEGIPSDVIAEGLVLRTDPAAGT
                     RLAKGEEVTIVPSSGRKRVSVPKVTGLEQAAAEKALEDVGLTVAVTQAASDTVEAGVV
                     MDQTPKPDEEVIAGTTVTITVSSGKGTAFVPNVEGLSEQVAIQRIRERGLVEQVQREQ
                     PGGSCPQPSGRVCRQSPSGGEQVERGSTVTIVVAEEPEPTTPPPPTDTPTPTPSPS"
BASE COUNT          363 a          815 c          798 g          375 t
ORIGIN      
        1 gccatggaat ccatggcggc gggcgaaaga cttaatttcc ccatgaagcc cttcctcgtg
       61 acgacctgcg acccttgcat gccccctaca accatttcga gccctcgcag ggtgacaggt
      121 ttgcccaaaa atcttccgga agacagaaaa tgcctggtgt acgatccgcg gcatgagcgg
      181 ggcccgtatc ctggtgatcg acaactacga ctcctttacc tacaacctgg tccaagagct
      241 ggccgagctg ggagcccggc cgacggtcgt gcgcaacgac gtgtggacgc tgggtcaggt
      301 gcgggacttt gccgccgacg ggatcgtgat ctcacccggc ccgggaaccc ccgccaagcc
      361 cggggacgtc gggatctcca acgacgtgat ccgcgagatg ggaccctcca cgcccgtgct
      421 gggcgtgtgc ctgggcaacc agtgcatcgg ctacgtctac ggcggccgga tcgtgcgagc
      481 cccgcagctg atgcacggca agacctcgcc gatctatcac cgcgaggaag ggattctgga
      541 aggcatcccg atcccgttcg aggcgacccg gtatcactcg ctgatcgtcg aacgcgcgtc
      601 cctgccggcc gaactgaagg tcacggccga gacgagcgac gagatcatca tgggcctgcg
      661 ccaccgcgag catccggtgg aaggcgtgca gttccatccc gagtcggtga tgaccggcga
      721 cggtatggac atgctgcgca actggctcgc ccgctgcggc gcgcccgcta gcacctgagc
      781 aggcagttca cggcccacac gatcagcacg gtcggcgtgc ccacgatcag cgcgaccagc
      841 gacgcggcca ggacccgccg ctcgccgcgg agccggctga agatgttgat cgcgcccagg
      901 ccgatcagcg cggccgacac cgcccaggca tagccgagcg ccgcgaggcg gccgaggttc
      961 tggccggcgg tgctgccctc gaacgcgcgc ggaagcgaca gcgcggccga cacggtgaag
     1021 aacgcgccga cgaacagggc cgcgccgagc aggatgcgcc agggcgcgcg ggcggggcag
     1081 atcgtcgagt cggtcatgcg ctcattcttc tccgatgcgg acacacgcgc tatcgggcgt
     1141 ggccctcatt ccgcatgccg aaactgccta tgcccgctag ctcggcgacg gtgtcggggt
     1201 cggcgtgtcg gtcggcggcg gcggcgtcgt cggctccggc tcctcggcga ccacgatggt
     1261 gacggtgctg ccgcgctcga cctgttcgcc gcctgagggc gactggcggc agacgcggcc
     1321 cgacggctgc gggcacgacc cacccggctg ctcgcgctgc acctgttcga ccagcccgcg
     1381 ttcgcggatc cgctggatcg ctacctgctc gctcaaccct tcgacgttgg gcacgaacgc
     1441 ggtgcccttg ccgctcgaaa ccgtgatggt caccgtcgtt ccggcgatga cctcctcgtc
     1501 gggcttgggc gtctggtcca tgacgacgcc ggcttcgacg gtgtcgctgg ccgcctgcgt
     1561 caccgcgacg gtaagaccga cgtcttcgag cgccttctcg gcggctgcct gctcgagccc
     1621 ggtgactttc ggcacggaga cgcgttttcg ccccgacgaa ggcacgatcg tgacctcctc
     1681 gcctttggcc agcctggttc cggcggccgg atcggtgcgc agcaccagcc cttcggcgat
     1741 cacgtcgctc ggaatgccct cgccgacctt ggtcttgaac ccggccagct cgagccggcc
     1801 ctgggcggcc tcgacggtga agccctccac ggacgggacc ggcacggtgc tcggggactt
     1861 cggaagcagc gcgagaagcg cccagatgat gatgccgatc atggcggccg cgatggccgc
     1921 gagcagcgcg atgcccgttc cgcgcttgcg gcgctcgggc gcgacgggac cgcgaaggat
     1981 cacggtctcg tctgccgtgc cgggcgcggt catcatcgcg gtctcgccca tcacgcgcgg
     2041 cgccgacacg gcctggccgc ggatcgcgcg ctcgagatcc tgccgcatct cctgcgcgga
     2101 ctgataccgg ttgtcggggt tcttcgacaa cgccttcatc acgatcgcct cgatctctgg
     2161 cgtgacgtcg gcgttgagcc gcgacggcgg gatcgggtct tccttgacgt gcttgtaggc
     2221 gatcgcgacc ggggaggctc ccgtgaacgg aacctgccgg gtgagcatct cgtagagcac
     2281 cacgccgagc gagtacaggt cgcttcgcgc gtcgaccggc gcggcttggg cctgctcggg
     2341 cgagaagtac t
//
LOCUS       DATLFO010000370         7396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_18990, whole genome shotgun sequence.
ACCESSION   DATLFO010000370 DATLFO010000000
VERSION     DATLFO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18990"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VM841_15180"
     CDS             complement(<1..435)
                     /locus_tag="VM841_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVL91563.1"
                     /translation="MHERKSLQLSLEDKMRALENLPLLANVSAKERRELAEKAHVLSF
                     AGGDVIVMEGEAGLGFYLILSGAVNVRRGGEIINRVEAGGFFGEVSLIEGVPRTADVL
                     ADGPTVCLGLLRSDFKRLLVREPRVAMTILEEEGRRLVGSPLV"
     gene            complement(438..1568)
                     /gene="holB"
                     /locus_tag="VM841_15185"
     CDS             complement(438..1568)
                     /gene="holB"
                     /locus_tag="VM841_15185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HVL91564.1"
                     /translation="MSVTGTAPAMWSRLVGQDAVVRALRDAAAAGNVGHALLFAGPEG
                     VGRRLAALALAASLNCDQGGCGDCAICAKVLRGAHPDVHLIVPEGQFILIGQIKGDGS
                     NRGVIGEAHRSAIEGKAKIFIIEDAERMNLASANALLKVLEEPPAGVVFVLMTGRPED
                     LPETVVSRCRRLDFSLLPEAAIREVLESHHGIAPDRAAWAARIGGNLARALRLAHDPD
                     APDRYDSHRAIPVRLADKGLSEAIVIADELRAEAEHVTARLRKRQAEEIAEAAEAEGE
                     SPRGRAARGSAAIRKRMETRHKRELRRAELDAIQAALDDIGSWYRDALVRAAEGERSR
                     MSPAAMLAALDRIEWTRRALERNAAAGLVLEALLAGLATARM"
     gene            complement(1565..3598)
                     /gene="tmk"
                     /locus_tag="VM841_15190"
     CDS             complement(1565..3598)
                     /gene="tmk"
                     /locus_tag="VM841_15190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVL91565.1"
                     /translation="MKEPYISDATFTAPSEHPSTLLGVLKSKHFRRLWAAMGVSSLGD
                     WLGLFALTLFVQELSGRPEFAVGGVLLFRVLPALLFGQFSGVLADRFDRRRLMITADV
                     MRALLIASVPWIHQLWVLFAVSAVMEMLSLLWTPAKDATIPNLVRREQLLMANQLSLI
                     TTYGTFPLSGALVALLAALSGVLAGTFDVLRDNPTYIAFFVDAATFLFSATMVATLPA
                     NVMRAKRARSEFSAQHAFRDLTEGLRYVKSNKIVRTLVIAAWTAFIGGAAVVSLGPIF
                     AKATQGGNAAAAAKAWGSLIVSVGSGLVGGMIFAGAVARRIPRERLFPIGLMLSGVTV
                     VLIASLQSVTPAIFITFFTGLGAGVAWVTIFTMLQERVDDRLRGRTFATLYTGVHLSL
                     FVGLAGWPLLAGAVGDHTLTIGTATIDLPGYRVMMWLGGLTVAYSGVRSLRIMRGYKG
                     GIVTPRLRGLEFRPKPVGGGARGGLFIVFEGVEGSGKSTQMEGLKAFLNEFGKEVLIT
                     REPGGTAIGERIRGLLLDPGANEMDAKAEALLYAAARAQHVAQVIRPALEEGKAVLSD
                     RYVDSSIAYQGLARGLGDDDIFRLNVWATDELMPDLVILLNVEAEVGLRRATGTPDRM
                     EREDLSFHRKVGEAYLQLAKAYPSRFVVIDASQPVEKVREQVERAVYNVLHDE"
     gene            complement(3679..5976)
                     /gene="topA"
                     /locus_tag="VM841_15195"
     CDS             complement(3679..5976)
                     /gene="topA"
                     /locus_tag="VM841_15195"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HVL91566.1"
                     /translation="MANARNLVIVESPTKAKTIGKYLGSDYDVRASMGHVRDLPKSTK
                     DGIGVKVNGDVSLDYIVTTKAKKFISDLKKAAKDAPTVFLATDHDREGEAIAWHIAEA
                     AKIPAEKARRVTFTEITQSAIREAFASPRDVDNNLVNAQQARRAVDRIVGYKLSPVLW
                     RKIGSRTSAGRVQSAALRLVVDREREIQAFVPVEYWTIEAALQTEKKEAFGARHPFLE
                     KEKFSLPNDESAQTVVDAVRPATWTVETVKRTERKRNPAPPFITSTLQQEASRKLGFS
                     AKRTMAVAQQLYEGIELGAEGSVGLITYMRTDSPHIAEQALGEINDVILDRFGKEYAL
                     PKPRQFKAKASRAQEAHEAVRPTEAQRGPETLAAFLDKDQLRLYTLIWQRAIASQMAQ
                     AIFDAVSVDIKAADYPFRSSGQTVRFDGFMRVYTEGRDDPSGEDDDESRTLPDLQEGQ
                     VLTLDTVEPVQHFTEPPPRFTEASLVKALEELGIGRPSTYAQIMSTLHDRKYVSSERK
                     RLQPTDLGFVICDFLTDVYPKVVDLEFTAEMENELDKIAEGEEPWEPMVRVFFSEVSD
                     ISERAAEKAERPSEKTDIDCPECGAETGAKMQKKWGRYGWFLSCERYPDCKARMKVAK
                     GEDGETTAAPRAEPEMTEIPCPLCSKPMLKRTGRFGEFLGCQDYPKCKGVKNLEDLDW
                     PEIQCPKCKQGHVVRKRTKRRKIFYGCNRYPECDYAMWEPVLPAPCPNCKGPLVADGD
                     DGAKCVACEKQFERSALDLPEPANA"
     gene            complement(6043..>7396)
                     /locus_tag="VM841_15200"
     CDS             complement(6043..>7396)
                     /locus_tag="VM841_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91567.1"
                     /translation="GRPVLRIVDGDATDADLVIHVPPGGRTPLLLTMPGDGFRGLMAG
                     GQSRRPGIVTPYDLSATIVRHFARPVPDGFVGGILVRDPGANPLDRARTLAGRMERDV
                     SFMQSLTAWTVVIGLGLGWLVAWLVALAGKRTLSQRLTVGASTVTIGFLIGLFVPSAR
                     GDVRAIPLVALVALAAWYPWRDREAAAMRFFVVAAGATAILAVIAAADPGGVVAHALW
                     GNPLVSWRLFGLQNFATAFIAGGAVVGATRLGLRPLPLGLLSAALIVVVGSPRIGANF
                     VAVLTFAFGAAVAVAVLRAGTLRPSAVAVAAGIALAAFALALLADAGDATSHGGAAAQ
                     QIGRGGAGALWDITIDRLRLNLRAINAFPGGWAWVVAFLALYAAMIRDARTRPGWPAA
                     RRAAVAAGALAGLAALGLEDSGFKTTAVLGFPALLLWTLAEAGRPLFPKETAGQRRRA
                     "
BASE COUNT         1161 a         2533 c         2578 g         1124 t
ORIGIN      
        1 taccaggggg ctgccgacca ggcggcggcc ctcttcttcc aaaatggtca tcgcgacgcg
       61 cggctcgcga acgagcaggc gcttgaagtc cgagcgaagc agacccaggc agaccgtcgg
      121 accgtcggcg agcacgtcgg cggtccgcgg gacgccttcg atgagcgaga cctcgccgaa
      181 gaacccgccc gcctcgacgc ggttgatgat ctcgccgccg cgacgcacgt tcaccgcgcc
      241 cgacaggatc aggtagaagc ccaggcccgc ctcgccttcc atcacgatca cgtcgccgcc
      301 ggcgaaggaa aggacgtggg ccttctcggc gagctcgcgg cgctccttgg cggagacgtt
      361 cgcgagcagc ggcaggttct cgagagcgcg catcttgtcc tcgaggctga gctgaagcga
      421 cttgcgctcg tgcacggcta catcctcgcg gttgccaggc ccgcgagcaa agcctccagc
      481 acaaggcccg ccgcggcgtt acgctcgagc gcgcggcggg tccattcgat ccggtcgagc
      541 gccgccagca tcgcggccgg gctcatccgg gaacgctcgc cttcggcggc acgcacgagc
      601 gcgtcgcgat accacgatcc gatgtcgtcg agcgccgcct ggatcgcgtc gagctcggcc
      661 cggcgaagct cgcgcttgtg gcgggtttcc atgcgcttgc ggatcgcggc ggatcctcgg
      721 gcggcacggc cgcggggtga ctcgccttcg gcttcggcgg cttccgcgat ctcttctgcc
      781 tggcgcttgc gaaggcgagc ggtgacatgc tcggcctcgg cgcgcagctc gtcggcgatc
      841 acgatcgcct ccgacagccc cttgtcggcc agccggaccg ggatcgcgcg atgcgagtcg
      901 tatcggtcgg gcgcatcggg atcgtgtgcg agccggagcg cgcgcgcgag gttgccgccg
      961 atgcgcgcgg cccaggcggc ccggtcggga gcgatgccgt gatggctttc gaggacctcg
     1021 cggatcgcgg cctcgggcag caggctgaag tcgaggcggc ggcagcgcga gacgacggtc
     1081 tcgggcagat cctcgggacg gccggtcatg agcacgaaaa ccacgcccgc gggcggctct
     1141 tcgagcacct tcaacagcgc gttggccgag gcgaggttca ttcgctcggc gtcctcgatg
     1201 atgaagatct tggccttgcc ttcgatggcg ctgcgatgcg cctcaccgat gacgccgcgg
     1261 ttggagccgt cgcctttgat ctggccgatc aagatgaact ggccttcggg cacgatcaga
     1321 tgcacgtccg ggtgcgcgcc gcgcagcacc ttcgcgcaga tcgcgcagtc gccgcagccg
     1381 ccctgatcgc agttaagcga cgcggcgagc gcgagcgccg cgaggcggcg gccgacgcct
     1441 tcgggtccgg cgaacagcag cgcgtgaccg acgtttcccg ccgcggccgc atcccgcagc
     1501 gcgcgcacga cggcatcctg gccgacgagt cgcgaccaca tcgcgggggc ggttccggtg
     1561 acgctcattc gtcgtgaaga acgttgtaga cggcgcgctc cacctgctcg cggaccttct
     1621 cgaccggctg ggacgcatcg atcaccacga agcggctcgg ataggccttg gcgagctgca
     1681 gataggcctc accgaccttg cggtggaacg acagatcctc tcgctccatc cggtcgggcg
     1741 tcccggtcgc gcgccgcagt cccacctcgg cctcaacgtt gagcaagatc accagatcgg
     1801 gcatcagctc gtcggtcgcc cagacgttca gccggaagat gtcgtcgtcg ccgaggccgc
     1861 gcgcgaggcc ctggtaggcg atcgaggaat cgacgtagcg gtcggacagc acggccttgc
     1921 cctcttcgag cgcaggccgg atcacctgcg cgacgtgctg ggcgcgcgcg gcggcgtaca
     1981 gcagggcctc cgccttggcg tccatctcgt tggcgccggg atccagcagg agcccgcgga
     2041 tccgttcgcc gatggcggtg ccgccgggct cgcgcgtgat cagcacttcc ttgccgaact
     2101 cgttcagaaa cgccttcagc ccttccatct gcgtgctctt tcccgagccc tcgacgcctt
     2161 cgaagacgat aaacaggccg ccgcgcgcgc cgccgccgac gggcttgggc cggaactcca
     2221 ggcctcgcag ccgcggcgtg acgatgcctc ccttgtagcc gcgcatgatc cgcagcgaac
     2281 gcacgcccga gtacgcgacg gtcaggccgc cgagccacat catcacgcgg taccccggca
     2341 ggtcgatcgt tgccgtgccg atcgtgagcg tgtggtcgcc cacggccccg gcgagcagcg
     2401 gccatccggc cagcccgacg aacagcgaca ggtgcacacc ggtgtagagc gtcgcgaagg
     2461 ttcggccgcg aagccggtcg tccacgcgct cttggagcat ggtgaagatc gtcacccagg
     2521 cgacgccggc gcccaggccg gtgaagaagg tgatgaagat cgccggcgtc accgactgca
     2581 gcgaggcgat gagcaccacg gtcacacccg acagcatcag cccgatcggg aacagccgtt
     2641 cccgggggat ccgtctcgca accgcaccgg cgaagatcat gccgcccacc aaacccgaac
     2701 cgaccgacac gatcagcgag ccccacgcct tggccgcggc cgcggcgttg ccgccttgcg
     2761 tggctttggc gaagatcggt cccagcgaca ccaccgccgc gccgccgatg aacgcggtcc
     2821 aggccgcgat caccagggtg cgcacaatct tgttggactt gacgtaccgc aggccttccg
     2881 taaggtcgcg gaaggcgtgc tgcgcgctga actcgctgcg cgcgcgcttg gcgcgcatca
     2941 cgttggcggg cagcgtggcg accatggtgg ccgagaacaa gaacgttgcg gcgtccacga
     3001 agaaggcgat gtaggtcggg ttgtctcgca gcacgtcgaa ggtgcccgcc agcacgcccg
     3061 acagcgcggc cagcagcgcg accagcgcgc ccgacagagg gaaggttcca taggtggtga
     3121 tcagcgagag ctgattggcc atcaagagct gctcgcgcct gaccaagttc gggatggtcg
     3181 cgtccttggc gggtgtccac agcaacgaca gcatctccat tacggccgac acggcgaaca
     3241 gcacccacag ctgatggatc cacgggacgc ttgcgatcag cagggcacgc atcacgtcgg
     3301 cggtgatcat cagccggcgg cggtcgaaac ggtcggccag cacgcccgag aactggccga
     3361 acagcagtgc gggcaggacg cggaacagca gcacgccgcc gacggcgaac tcgggacggc
     3421 ccgatagctc ctgcacgaac agcgtcaggg cgaacaggcc cagccagtcg cccaggctgg
     3481 acacgcccat ggcggcccat agacgccgga agtgcttgct cttcaggacg ccgaggagcg
     3541 tcgagggatg ctcgctcggc gccgtaaacg tcgcgtcgct gatgtacggc tccttgatga
     3601 gacggtggga aggccggaaa tcaggctagc aggccgaccg cggcgtgacg tatcgcccgg
     3661 acggcaccgg ggcgggcctc aggcgttggc gggctcgggc aaatccaagg ccgaccgctc
     3721 gaactgcttt tcgcaggcca cgcacttggc gccgtcgtcg ccgtcggcca ccagcgggcc
     3781 cttgcagttc gggcacggcg cgggcagcac gggctcccac atcgcgtagt cgcactcggg
     3841 gtagcggttg cagccgtaga agatcttgcg gcgcttcgtg cgcttgcgca cgacgtgacc
     3901 ctgcttgcac ttgggacact ggatctcggg ccagtcgagg tcctcgaggt tcttgacacc
     3961 cttgcacttc gggtagtcct ggcagccgag gaactcgccg aagcggccgg tgcgcttgag
     4021 catcggcttg ctgcacagcg ggcaagggat ctcggtcatc tccggctcgg cgcgcggcgc
     4081 cgcggtggtc tcgccgtcct cgcccttggc gaccttcatg cgcgccttgc agtccgggta
     4141 gcgctcgcag gagaggaacc agccgtagcg gccccacttt ttctgcatct tcgcgcccgt
     4201 ctcggcgccg cactcggggc agtcgatgtc ggtcttttcg ctgggacgct cggccttctc
     4261 ggcggcgcgc tcgctgatgt cgctgacctc gctgaagaag acgcggacca tcggctccca
     4321 cggctcttcg ccttcggcga tcttgtcgag ctcgttctcc atctcggccg tgaactccaa
     4381 atcgacgacc ttgggataca cgtcggtcag gaagtcgcag atcacgaaac cgagatcggt
     4441 cggctgcagc cgcttgcgtt cgctgctgac gtacttgcgg tcgtgcagcg tggacatgat
     4501 ctgcgcgtac gtggacggcc ggccgatgcc gagctcttcg agcgccttga ccagcgacgc
     4561 ctcggtgaag cgcggcggcg gctcggtgaa gtgctggacc ggctcgacgg tgtcgagcgt
     4621 gagcacctgg ccttcttgaa ggtcgggcag cgtgcggctc tcgtcgtcgt cctcgccgct
     4681 cgggtcgtcg cgcccttcgg tgtagacgcg catgaagccg tcgaagcgca ccgtctggcc
     4741 ggacgagcgg aacgggtagt cggcggcctt gatgtccacg ctgaccgcgt cgaagatcgc
     4801 ctgcgccatc tggctggcga tcgcgcgctg ccagatcagc gtgtagaggc gaagctgatc
     4861 cttgtcgagg aacgccgcga gcgtctcggg gccgcgctgc gcctcggtcg gccgcaccgc
     4921 ttcgtgggct tcctgcgcgc gggacgcctt ggccttgaac tggcgtggct tgggcagcgc
     4981 gtactcctta ccgaaacgat ccaggatcac gtcgttgatc tcgcccagcg cctgttcggc
     5041 gatgtgcggc gagtcggtac gcatataggt gatcagaccg acgctgcctt cggcgccgag
     5101 ttcgatccct tcgtagagct gctgcgcgac ggccatggtg cgcttggccg agaagccgag
     5161 cttgcgcgag gcctcctgct ggagggtcga ggtgatgaac ggcggcgccg gattgcgctt
     5221 gcgctcggtc cgcttaacgg tctcgacggt ccaggtcgcc ggacgcaccg cgtccacgac
     5281 ggtctgcgcc gactcgtcgt tgggcagcga gaacttctcc ttctccagga aggggtgccg
     5341 cgcgccgaac gcctctttct tttcggtctg cagcgcggcc tcgatcgtcc agtactcgac
     5401 cgggacgaac gcctggatct cgcgctcgcg gtccacgacc aggcggagcg cggcggactg
     5461 gacgcggccg gcgctggtgc ggctgccgat cttgcgccac agcaccggcg aaagcttgta
     5521 gccgacgatg cggtccacgg cgcggcgggc ctgctgcgcg ttgacgaggt tgttgtccac
     5581 gtcgcgcggc gatgcgaacg cctcgcggat cgcggactgc gtgatctcgg tgaaggtgac
     5641 cctgcgggcc ttttcggccg ggatcttcgc ggcctcggcg atatgccacg cgatcgcttc
     5701 gccttcgcgg tcgtggtcgg tggcgaggaa gacggtcggg gcgtccttgg cggccttctt
     5761 caggtcgctg atgaacttct tggccttggt tgtgacgatg tagtcgagcg agacgtcgcc
     5821 gttgaccttg acgccgatgc cgtccttggt ggacttgggc aggtcgcgga catggcccat
     5881 cgaggcgcgg acgtcgtagt ccgagccgag gtatttgccg atcgtcttcg ctttggtcgg
     5941 cgactcgacg atgaccaggt tgcgtgcgtt cgccatagtt gcggaagcct tagagagagt
     6001 gccctcggga cgggccatgt aacgcgtcaa cggcgccgca tgtcaagcac gacgcctctg
     6061 acctgcggtt tctttcggga ataggggccg gccggcttcg gcaagggtcc acagaagcaa
     6121 agccgggaac cccaggaccg cggtggtctt gaagccggag tcctcgagcc cgagggcggc
     6181 gaggccggcg agcgcgccgg ccgcgacggc ggccctgcgg gccgccggcc atccgggccg
     6241 ggtccgggca tcgcggatca tcgccgcgta gagggcgagg aacgccacca cccaggccca
     6301 gcccccgggg aaggcgttga tcgcacgcag gttgagccgg agccggtcga tggtgatgtc
     6361 ccagagcgcc cccgcgccgc cccgcccgat ctgctgcgcg gccgctccgc cgtgggaggt
     6421 cgcgtcgccg gcgtcggcca gcagcgcgag cgcgaacgcg gcaagcgcga tgccggcggc
     6481 gacggcgacc gcggacgggc gcagcgtgcc ggcgcgaagc accgcgaccg cgacggcggc
     6541 accgaaggcg aaggtcagca ccgcgacgaa gttcgcgccg atacggggcg agccgaccac
     6601 gacgatcagc gcggccgaca gcaatcccag cggcagcggc cgcagcccga ggcgcgtcgc
     6661 tccgacgacg gctcccccgg cgatgaacgc cgttgcgaag ttctgcagcc cgaacagccg
     6721 ccacgacacc agcgggttgc cccacaaggc gtgggcgacg acgccgccgg gatcggcggc
     6781 cgcgatcacg gccaagatcg cggtcgcgcc cgcggcgacg acgaagaatc gcatcgcggc
     6841 agcttcgcgg tcgcgccacg gataccaagc ggcgagcgcg accaacgcaa cgagcggaat
     6901 cgcgcgcacg tcgccgcgcg ccgacggcac gaacagcccg atcaggaacc cgattgtcac
     6961 ggtcgatgcg cccaccgtca gccgctgcga cagcgttcgc ttcccggcga gcgccacgag
     7021 ccaggccacg agccacccca agccgagccc gatcacgacc gtccatgcgg tcagcgactg
     7081 catgaacgac acgtcacgtt ccatccgtcc ggccaacgtg cgtgcccgat cgagcgggtt
     7141 cgcgcccggg tcgcgcacca ggatgccgcc gacgaagccg tcgggaaccg ggcgagcgaa
     7201 gtgtcgcacg atggttgcag aaagatcgta gggcgtgacg atccccggcc ggcgcgactg
     7261 cccacccgcc atgagcccgc ggaagccgtc gccgggcatc gtcagcagca gcggcgtccg
     7321 gccgccgggc gggacatgga tgaccagatc cgcgtcggtg gcgtcgccgt ccacgatgcg
     7381 aagcaccggc cggccc
//
LOCUS       DATLFO010000371         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_19103, whole genome shotgun sequence.
ACCESSION   DATLFO010000371 DATLFO010000000
VERSION     DATLFO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19103"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VM841_15205"
     CDS             complement(<1..268)
                     /locus_tag="VM841_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91568.1"
                     /translation="MAQTSSRASRHLSALAALTAGVAAVATLGAQSSGGKWWPGYSGG
                     ADNSRFFASRQINKSNVNQLQVAWTYPYGDTTFAPVAVRGAIYGR"
     gene            374..2290
                     /locus_tag="VM841_15210"
     CDS             374..2290
                     /locus_tag="VM841_15210"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVL91569.1"
                     /translation="MVQNATFIRRACMGGLAGLTLVLTGCSSDEPRWRDVNQRVIDEH
                     EVTGRARELPDVTVPTLLADGEPVERTKLPIITIAPGVTAALGWGRGALLERVEMQPG
                     AAYPSQTLAEELIIIVEDGSATVEFAGQTALLEKDHVLYLQPGTTRSVKAGANGWKAF
                     EVYSPVRLDHLALAGQNTSGVNPTFPDQGVTPSLLPGAVVNVNDIQWTPITDPVGGQA
                     YRRSTAHSRLIWGKNAQVSLVRMDPGSEFPLHIHPEDQLTHTIRGTLDQGVMDRSFPA
                     SGAAGHMLYLPGGMVHSAQLGDTGGDQLDVFWPVRPDYVERARKQQALYEQIVAGDAK
                     PAKLAQGFTFTEGPTWLKGKLYFSDMFFQNPAANDWTGSPARSRLIVMERDGKWRVLS
                     SGMQSNGTLAARNGNLLVCDMFGHRVVEIHPATGRVLRVVLDKVNGQPIDGPNDLVMD
                     AKGGLYITDPQFTPEAQKSQPGKQVYYVAPDGTAKVVIGPGEFAMPNGVELSPDGKTL
                     YVNNTWQQPGENFVWAYDVAADGSLSNKRQFAMLHLTPDVLSAANAADRFDSRADGTA
                     VDTDGRYYVATKSGVQIFLPDGTYAGTIWVPQYPVSVTFGGPNNDVLYMVGESSAWAI
                     QTKVRGFRHPEGMN"
BASE COUNT          417 a          754 c          788 g          408 t
ORIGIN      
        1 cgcggccgta gatcgcgccg cgaacggcca ccggggcgaa ggtcgtgtcg ccataggggt
       61 aggtccaggc cacctgcagc tggttgacgt tggacttgtt gatctgccgt gacgcgaaaa
      121 agcgggagtt gtcagcgccg ccgctgtatc cgggccacca cttgccgccc gacgactgcg
      181 caccgagggt ggcgaccgcc gccacgccgg cggtcagggc ggcgagtgcc gagaggtgcc
      241 gcgaggcccg agagcttgtc tgcgccatag gcgcggtatc atagccgacg cactcgtcgt
      301 tgcacattgc gcaacacggt ttgccggatt ttttcgtcgc gcacgattga ttgcgagtag
      361 gatggcgccc tcgatggtcc agaacgcgac tttcatccgc cgcgcctgca tgggcgggct
      421 ggcgggactg accttggtcc ttaccggttg ctccagcgac gaaccgcggt ggcgagatgt
      481 gaatcagcgc gtcatcgacg agcacgaggt gaccggacgc gcccgcgagc tgccggacgt
      541 cactgtgccc acgctcctcg ccgacgggga gccggtcgaa cgcaccaagc tgccgatcat
      601 cacgatcgcg ccaggcgtga cggccgcgct cggctggggc cgcggtgcgc tcctcgagcg
      661 ggtcgagatg cagcccggcg ccgcctatcc gtcgcagacg cttgccgagg aactgatcat
      721 catcgtcgag gacggttcgg cgacagttga attcgccggc cagaccgccc tactggaaaa
      781 ggaccacgtg ctctacctgc agcccggtac cacgcgatcg gtcaaggccg gcgcgaacgg
      841 gtggaaggcg ttcgaggtct attcaccggt gcggctcgat catctcgcat tggccgggca
      901 gaacacctcc ggtgtgaatc cgacgtttcc ggatcagggc gtcacgccat ctctcctgcc
      961 gggcgcggtc gtcaatgtca acgacattca gtggacaccc attaccgatc ccgttggcgg
     1021 gcaggcgtac cgtcgcagca cggcccactc acggctcatc tgggggaaga acgcgcaggt
     1081 cagcctggtg cgcatggatc cgggatccga atttccgctg cacatccatc ccgaggatca
     1141 gttgacgcac acgatccggg ggacgctcga ccagggcgtg atggaccgat cgtttcccgc
     1201 atccggcgcc gccggtcaca tgctgtacct gcctggcggc atggtgcatt cggcgcaact
     1261 cggtgacacc ggcggcgatc agctcgatgt gttctggccc gtgcggcccg attatgtcga
     1321 gcgcgcgcgc aagcagcagg cgctctatga gcagatcgtg gctggcgacg cgaagccggc
     1381 gaagctcgcc cagggcttta cgtttaccga agggccgacg tggctgaaag gaaagctgta
     1441 cttctccgac atgttttttc agaaccccgc cgccaacgat tggaccggga gcccggcgcg
     1501 cagccgcctg atcgtgatgg agcgcgacgg caaatggcgc gtgctctcga gcggcatgca
     1561 gtcgaacggt acgctggcgg cgaggaacgg gaatctgctc gtgtgcgaca tgttcggcca
     1621 ccgtgtcgtg gagatacatc cggctaccgg gcgcgtgctg cgcgtcgtgc tcgacaaggt
     1681 gaacggccag ccgatcgacg gcccgaacga cctggtcatg gacgccaagg gtggccttta
     1741 catcaccgac ccgcagttca cgccggaagc gcagaagagc cagcctggca aacaggtgta
     1801 ttacgtcgcg ccagacggca cggcgaaggt ggtcatcggt ccgggcgaat tcgcgatgcc
     1861 caacggtgtc gagctctcgc ccgacggcaa gacactctac gtgaacaaca cctggcagca
     1921 gccgggtgag aacttcgtct gggcgtatga cgtggccgcg gatggctcgc tgtcgaacaa
     1981 gcggcagttc gcgatgctgc acctgacgcc ggacgtgctc agcgcggcca atgctgccga
     2041 ccgcttcgat tcgcgcgcgg acggtacggc ggtcgacaca gatggccggt actatgtggc
     2101 caccaagagc ggcgtccaga tattccttcc cgatggcact tacgccggca cgatctgggt
     2161 gccgcaatat cctgtcagcg tgacattcgg cggcccgaac aacgacgtgc tctacatggt
     2221 gggtgaatcg tccgcatggg cgattcaaac gaaagtccgc ggcttccgtc atccggaggg
     2281 catgaactga tgagaccttt gctgcttgtc gtcctcgctg tcgctgttcc gtctctctgg
     2341 ccggcgcacc cggccgcgca gcagcag
//
LOCUS       DATLFO010000372         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_19286, whole genome shotgun sequence.
ACCESSION   DATLFO010000372 DATLFO010000000
VERSION     DATLFO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19286"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..245)
                     /locus_tag="VM841_15215"
     CDS             complement(3..245)
                     /locus_tag="VM841_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91570.1"
                     /translation="MRDVGDEVAAHLLAAALLRHVGDQRESGIAEGHDPDREPPPVRH
                     VQLTLVGDRLAGGTGLLRELGQAKRGERRRADRRRA"
     gene            complement(292..681)
                     /locus_tag="VM841_15220"
     CDS             complement(292..681)
                     /locus_tag="VM841_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVL91571.1"
                     /translation="MPGTDPPAGRSGWGSSLFGRRERDTQQRLRAEADRALAEARDEA
                     AALERILDAMDQGAVVLDAAGDPLFANRAAREILGIGPRRTSPAFASLRLPELAKQAR
                     AAGEPIADERELHVPNRRRQGLRGSRQ"
     gene            complement(699..1562)
                     /gene="pstB"
                     /locus_tag="VM841_15225"
     CDS             complement(699..1562)
                     /gene="pstB"
                     /locus_tag="VM841_15225"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HVL91572.1"
                     /translation="MPRLGNLLADRRNETEKRTIILPDQSQATEPVFRLEDVSVYYGD
                     FRAVRDVTLDIAQHQITALIGPSGCGKSTLIRVLNRMNDLIPGARVEGKVVYHDQDLY
                     DPAVDPVEVRRRIGMVFQRPNPFPKSIYENIAFGLRVNGLTDDIDDRVEEALKAAALW
                     EEVKADLKKSAFDLSGGQQQRLCIARAVAVQPDVILMDEPCSALDPISTARVEDLMFA
                     LKDRYSIVIVTHNMQQAARVSEMTAFFTTEITGDDERRTGVLVEYDRTETLFTRPSDE
                     RTEAYITGRFG"
     gene            complement(1569..>2076)
                     /locus_tag="VM841_15230"
     CDS             complement(1569..>2076)
                     /locus_tag="VM841_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVL91573.1"
                     /translation="SIVYGLLGLAAFVRFMHMGRTVLAGALTLVLLILPVIIVSAREA
                     LRAVPDSIRHGALAVGATTWQTVSRHTLPAAIPGIMTGMILGLSRAIGETAPLITMGA
                     LTYVNFVPTGPLDRFTAMPIQIFNWTSRPQAEFQNVAAAGIILLLGALLLMNAVAVFL
                     RNRYERRW"
BASE COUNT          337 a          708 c          704 g          327 t
ORIGIN      
        1 gctcacgctc gtcggcgatc ggctcgccgg cgttcgcctc gcttcgcctg cccgagctcg
       61 cgaagcaagc ccgtgccgcc ggcgagccga tcgccgacga gcgtgagctg cacgtgccga
      121 accggcggcg gctcgcgatc cgggtcgtgc ccttcggcga tgccgctctc gcgctggtcg
      181 ccgacgtgac gcaggagcgc cgcagcgagc aggtgcgccg cgacttcgtc gccaacgtct
      241 cgcacgagct caagacgccg atcgcgggca tcgccctgat cgccgagcag ctcactgacg
      301 cgatccgcga agaccctgac gccgccggtt cggcacgtgc agctcacgct cgtcggcgat
      361 cggctcgccg gcggcacggg cttgcttcgc gagctcgggc aggcgaagcg aggcgaacgc
      421 cggcgaggtg cggcgcggcc cgatgcccag gatctcgcgc gccgcgcgat tggcgaacag
      481 cgggtcgccg gcggcgtcga gcaccacggc accttgatcc atcgcgtcga ggatgcgctc
      541 gagcgcggcg gcttcgtcgc gtgcctcggc cagcgcgcga tccgcctcgg cacgaagccg
      601 ctgctgcgtg tcgcgctcgc ggcggccgaa cagcgacgag ccccaaccgg aacggcccgc
      661 gggtggatcc gtgccgggca tcgcgatcgc cgggtccttt atccgaaccg ccctgtgatg
      721 taggcctcgg ttcgctcgtc cgacggacgg gtaaagagcg tctcggtgcg gtcgtactcg
      781 accagcacac cggtgcggcg ctcgtcgtcg ccggtaatct cggtggtgaa gaacgccgtc
      841 atctccgata cacgcgcggc ctgttgcatg ttgtgcgtga cgatcacgat cgagtaccgg
      901 tccttcagcg cgaacatcag gtcctcgacg cgcgcggtcg agatcgggtc gagcgccgag
      961 cacggctcgt ccatcaaaat cacgtccggc tggacggcaa cggcgcgcgc gatgcagagc
     1021 cgctgctgct ggccgcccga caggtcgaag gcgctcttct tcagatccgc cttgacctct
     1081 tcccacagcg cggctgcttt cagcgcctcc tcgacacggt cgtcgatgtc gtcggtcaag
     1141 ccgttgacgc gaagtccgaa cgcgatgttt tcgtagatcg acttcgggaa cggattcggc
     1201 cgctggaaga ccatgccgat gcggcgacgc acctcgaccg ggtccacggc cgggtcgtac
     1261 agatcctgat cgtggtagac gaccttgcct tcgacgcggg ccccggggat caggtcgttc
     1321 atccggttga gcacccggat cagcgtggac ttgccgcagc ccgagggccc gatcagcgcg
     1381 gtgatctgat gctgtgcgat gtcgagtgtg acgtcgcgga cggcgcggaa atccccgtag
     1441 tagacggaga catcctccaa acggaagacg ggttctgtcg cctgcgactg atccggcaag
     1501 atgatggtcc tcttctcggt ctcgttcctg cggtccgcga gcaggttacc caacctgggc
     1561 atgttcgatc accaacgcct ctcgtagcgg ttgcggagga acacggctac cgcgttcatc
     1621 agcagcagcg ctcccagcag caagatgatg cccgcggcgg ccacgttctg gaactctgcc
     1681 tgcgggcggg acgtccagtt gaagatctga atcggcatgg cggtgaagcg atcaagcggg
     1741 ccggtgggga cgaagttcac ataggtcagc gcgcccatcg tgatcagcgg cgcggtctcg
     1801 ccgatcgccc gggacaggcc caggatcatg ccggtcatga tgcccgggat cgcggccggc
     1861 agcgtatgac gcgacaccgt ctgccacgtg gtcgcgccca cggccagggc gccgtgccgg
     1921 atcgagtcgg gcaccgcgcg caaggcctcg cgcgccgaca cgatgatcac cggcaggatc
     1981 agcaggacca aggtcagcgc gccggccagc acggtgcggc ccatatgcat aaaccgaacg
     2041 aaagcggcca gacccagcag cccgtacacg attgac
//
LOCUS       DATLFO010000373         5213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_69069, whole genome shotgun sequence.
ACCESSION   DATLFO010000373 DATLFO010000000
VERSION     DATLFO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69069"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VM841_15235"
     CDS             <1..487
                     /locus_tag="VM841_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HVL91574.1"
                     /translation="FSIGNTETVVASAVRRAVRAGEPEAVPRISDLPSVLQSSTGRVE
                     FEAFEEGREADILGRMLRQASLDTFRRRLAGIDIVPLQQRFDEGLSLETGEMVSADRV
                     LEQIGQFPGLAKAMQRLGIEEESPALAAAVIEFVLEGMHLSKRLNKAASGEGRVRYAG
                     E"
     gene            488..778
                     /locus_tag="VM841_15240"
     CDS             488..778
                     /locus_tag="VM841_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HVL91575.1"
                     /translation="MLAARLRNIARSVKREVDVYQLVLKDPRTPPKARWLLGLAVGYI
                     VLPFDFIPDFIPGLGQLDDALIVPTLIKLALRSIPPEVVEDARARVKGTEAG"
     gene            complement(985..1665)
                     /locus_tag="VM841_15245"
     CDS             complement(985..1665)
                     /locus_tag="VM841_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL91576.1"
                     /translation="MQDRAAKEALFDAFAAVAKALASGRRAEIVDVLAQGPRSVDELS
                     RQIGQSVANTSHHLQALAAGGLVRTTREGTRVIYRLAGPAVVALWRALRDVAAAHVGE
                     LERLAQAYLGGRDGIEPVPLDELSRRLGSGGVVVLDVRPAAEFAAGHIEGARSIPIEE
                     LSRRLKELPKSKRIIAYCRGPYCVFADDAVRLLRSKGFSATRMADGFPEWRDAGLPVT
                     SGTARPTA"
     gene            1782..2288
                     /locus_tag="VM841_15250"
     CDS             1782..2288
                     /locus_tag="VM841_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91577.1"
                     /translation="MATIARPAERAGTAEARLRLGSGIMAAAGIGFVGYAALFLIRNY
                     TDSFLEMGIGPAQVDVGKEQIIGFSPSLYHYVGHLHIAVSGFIAATGIAVASLAWFGV
                     RRGMTWAWVTAVAAPVVGLAVALPAHFPNGFDTAGHLGLIYANTALFVVGALLALAAL
                     RSAHEGRR"
     gene            complement(2349..3386)
                     /locus_tag="VM841_15255"
     CDS             complement(2349..3386)
                     /locus_tag="VM841_15255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVL91578.1"
                     /translation="MRRTNRVLSLILGTLLGIGLLPPAVAEDAMPMRVTLESISYDVE
                     PGTTVGALMSRAGVETKRANLVSLKGRVLSAGTVEGRILVDGVSSSRDRLLEDGDAVV
                     LEAAADRKESATRSTAPAAAVAGNPVRGIPANYGVSVITFRGTVSGETVRVVTPAGAD
                     KKSAVAITFDDGPDPTFTPQILAILNRYKVKATFFWTGRNIARYPKIVAAARAAGHSI
                     QNHTWNHENLARAGFAGQEKAIVAQTREMRKLGLPFPKWMRPPYGSFDPWTLKIAAKH
                     GMRTILWTIDTVDYRRPPAMTIAKRVLGQVRPGSVVLMHDGGGSRAATVEALPIILDA
                     LLKRGYRPVAL"
     gene            3437..>5213
                     /locus_tag="VM841_15260"
     CDS             3437..>5213
                     /locus_tag="VM841_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVL91579.1"
                     /translation="MPGYRYSRWDGTQDDPLGPDLDVPGIVDALSDELLEGGDPAEAI
                     DRLMRRGLPGRFGGLDAIRQRLREARLAAQQEGRLDGVLEQAREELDAILENERAELS
                     FRDDPEARMREGFLESLPPDPAGRIGELKEYDFVSPVAAERFKQLLEKLRQEMLGAYV
                     KSMAGAMQQMTPEDIAAMREMLAELNRMIDQRRRGVGPSQDEFNEFMKRHGRFFPENP
                     RTLDELLEALARRAAALGRLMASLSPEQRAELEALSQQLLSDMDLDMQVNQLSQSLHE
                     MFPDLGWDQQARMGGDQPLGLGEALQAIERLGDYEELERTLRGDYPGASLEDVDLDQV
                     RRTIGEDAARDLEKLRRVERALEEAGVVARRDGDWQLTPRGIRALGQRALATVFSKLR
                     DDRAGKHYAPQTGAGGEPTGATRTWRFGDHFRIDVRRSVQNAVLREGVTHGPVHVTAK
                     DFEIAEMETRTRNATVLLLDMSFSMPMRGNWAGAKRMALALHALISSQYPDDRLDIVG
                     FSDYARVMSPEDLANVGWEHVYGTNMEHAFNLAGRLLSKYPGSNKQVLLVTDGEPTAH
                     IIDGTDQVFFHWPPVRETLEKTYKEA"
BASE COUNT          840 a         1807 c         1788 g          778 t
ORIGIN      
        1 cttctcgatc ggcaacaccg agaccgtcgt cgcgtcggcg gtgcgccgcg ccgtgcgcgc
       61 cggcgagccc gaggcggtgc cgcgcatctc ggacctgcct tcggtgctcc aatcctcgac
      121 cggccgcgtg gagttcgaag ccttcgagga aggccgcgag gccgacatcc tcggccgtat
      181 gctgcgccag gcgtcgctgg acaccttccg ccgccggctc gccggcatcg acatcgtgcc
      241 gttgcagcag cgcttcgacg aaggcttgtc gctcgagacc ggcgagatgg tctcggcgga
      301 tcgtgtactc gagcagatcg ggcagttccc gggcctggcg aaagcgatgc agcgcctggg
      361 catcgaggag gagtcgccgg cgctcgcggc cgcggtgatc gagttcgtgc ttgaaggcat
      421 gcacctgtcg aagcggctca acaaggcggc cagcggcgag ggccgcgtcc gttacgcggg
      481 ggagtagatg ctcgcggccc gtctccgcaa catcgcccgc tcggtcaagc gcgaggtgga
      541 cgtgtatcag ctcgtcctca aggatccgcg cacgccgccc aaagcgcggt ggctgctcgg
      601 cctggcggtc ggctacatcg tgctgccgtt cgacttcatc cccgacttca tcccggggct
      661 cggccagctc gacgatgcgc tgatcgtgcc cacgctgatc aagctggcgc tgcgctcgat
      721 cccgcccgag gtcgtcgaag acgcgcgcgc ccgcgtcaag ggcacggagg ccggctagat
      781 cccgccaggc ttccggcggg ccagcgcgcc accatcgtcg cggccctcga tacgcaggtc
      841 gagccctcgc ggtccaccga cgcgcgcgcg gaagcaagcc gttcatcgag ccccggcacg
      901 ggcgtacgcc gggatcctca gccgagtcca cgaacggccg tgaggccgct gcggaaatgg
      961 attgggatca tgagtttggc gagtctaggc ggtcggccgc gcggttccgg aggtcacggg
     1021 cagccctgcg tcccgccatt cagggaatcc gtcggccatc cgcgtcgcgc tgaagccttt
     1081 cgagcggagc aagcgaaccg cgtcgtcggc gaagacgcag taggggccgc gacagtaagc
     1141 gatgatgcgc ttggacttgg gcagctcctt caagcggcga gagagctctt cgatcgggat
     1201 cgaccgcgcg ccttcgatgt gaccggccgc gaactcggcc gcgggccgta cgtccagcac
     1261 caccacgccg ccgcttccga ggcgccggga gagctcgtcg agcgggaccg gctcgatccc
     1321 gtcccggccc cccaggtacg cctgggcaag ccgttccagc tcgccgacgt gcgccgccgc
     1381 cacgtcgcgc agcgctcgcc agagcgcgac gacggccggc ccggcgagcc ggtagatcac
     1441 ccgagtgccc tccctggtgg ttcgcacgag gccgccggcg gccagggcct gcaggtgatg
     1501 cgaggtgttc gcgacgctct ggccgatctg gcgggaaagc tcgtccacgg agcgcggccc
     1561 ttgggcgagc acgtccacga tctcggcccg ccggccgctc gccagcgcct tggcgacggc
     1621 ggcgaacgca tcgaacaaag cctctttcgc ggcgcggtcc tgcatgaacg gcacgctacg
     1681 cgtccggatc gcaaattgtc aaatcttcaa gtgactactt gacaatgctc gatcacaagc
     1741 gtagaaccaa ggaagagcgc accccggaag gagggttcga catggcgacc atcgcacgcc
     1801 cggcggaacg agccggaacc gccgaggcac gactccggct cggctcgggg atcatggccg
     1861 cggccggcat cggtttcgtc gggtacgcag cgctgttcct gatccgcaac tacaccgact
     1921 cgttcctcga gatgggcatc ggtcccgccc aggtggacgt cggcaaggag cagatcatcg
     1981 ggttcagccc ctcgctgtac cactacgtgg ggcatctgca catcgccgtc tcgggcttca
     2041 tcgccgcgac cggcatcgcc gtcgcctcgc tcgcgtggtt cggcgtccgc aggggcatga
     2101 cctgggcatg ggtcaccgcg gtcgcggcgc cggtcgtggg cctcgccgtc gcgctcccgg
     2161 cccacttccc caacggattc gacaccgcgg gtcacctcgg cctgatctac gccaacaccg
     2221 cgctgttcgt ggtcggcgcg ctgctcgcgc tggcggccct tcgatcggca cacgagggac
     2281 ggcgatagga gctcgcgtcg ccccggcgtc cggtgatgcg ggcgccgggg cctctccgtc
     2341 cgccggacct agagcgcgac gggccggtac ccgcgcttga gcagcgcatc caagatgatc
     2401 ggcaacgcct cgacggtcgc ggcgcgcgag ccgccgccat cgtgcatcag aaccacgctg
     2461 ccgggccgca cctggccgag cacgcgcttg gcgatcgtca tcgcgggagg ccgccgatag
     2521 tccaccgtgt cgatggtcca aaggatcgtg cgcatgccgt gcttggccgc gatcttgagc
     2581 gtccacggat cgaaggatcc gtagggcgga cgcatccact tcgggaaggg caggccgagc
     2641 tttcgcatct cgcgggtctg cgccacgatc gccttctctt ggcccgcgaa accggcacgc
     2701 gcgaggttct cgtgattcca ggtgtgattc tggatcgagt ggcccgccgc gcgcgccgcc
     2761 gcgacgatct tgggatagcg ggcgatgtta cggccggtcc agaagaacgt ggccttgacc
     2821 ttgtagcggt tcaggatcgc caggatctgc ggcgtaaagg tcgggtcggg accgtcgtcg
     2881 aaggtgatcg cgaccgccga ctttttgtcc gcacccgcgg gcgtgacgac gcgcaccgtc
     2941 tcgcccgaga ccgttccgcg gaaagtgatc acactcacgc cgtagttcgc gggaatgccc
     3001 cggacgggat ttcccgcgac cgcggcggcc ggcgcggtgg aacgagtcgc gctctccttg
     3061 cggtcggcgg cggcctccaa gaccaccgcg tcgccgtcct cgagcaggcg atcgcgcgac
     3121 gaggaaaccc cgtcgaccag gatccggcct tccacggtgc cggccgaaag cacgcgaccc
     3181 ttcagggaca ccaggttggc ccgcttcgtc tccacgccgg cgcggctcat caacgcgccg
     3241 accgtggtgc ccggctccac gtcgtaggag atgctctcga gcgtgacgcg catcggcatc
     3301 gcgtcctcgg cgacggcagg tggcagcagc ccgatcccga gcagcgttcc gagtatgagc
     3361 gacaaaaccc gattcgtgcg gcgcatcgca atctccccct gcactgcgtg gctcgaaact
     3421 ctacgataaa ggagccatgc ccggctaccg gtactcccgt tgggacggaa cccaggacga
     3481 tccgctgggg cccgacctgg acgtgcccgg gatcgtggat gccctatccg acgagctgct
     3541 agaaggaggc gatccggcag aggcgatcga ccggctgatg cgccggggcc ttccaggccg
     3601 cttcggcggg ctggacgcga tccggcagcg gctccgcgag gcccgcctcg ccgcacagca
     3661 ggaaggccgc ctcgacggcg tgctcgaaca agcccgcgaa gagctggacg cgatcctgga
     3721 gaacgagcgt gccgagctgt ccttccgcga cgaccccgag gcgcgcatgc gggaaggatt
     3781 cctcgaatcg cttccgcccg acccggccgg acgcatcggc gagctgaagg agtacgactt
     3841 cgtctcgccc gtggccgccg aacgcttcaa gcagctgctc gagaagctgc ggcaagagat
     3901 gctcggcgcg tacgtgaagt cgatggccgg cgcgatgcag cagatgacgc ccgaggacat
     3961 cgcggcaatg cgggagatgc tcgccgaact caaccgcatg atcgatcaga ggcgtcgcgg
     4021 ggtcggcccg agccaggatg agttcaacga gttcatgaaa cgtcacgggc gcttcttccc
     4081 cgagaacccg cgcacgctcg acgagctgct cgaggcactc gcgcggcgcg ccgcggcgct
     4141 gggccggctg atggcgtcgc tgtcgcccga acagcgcgcc gagctggagg cgctgtcgca
     4201 gcaactgctg tcggacatgg atctggacat gcaggtcaac cagctttcgc agtcgctgca
     4261 cgagatgttc ccggatctcg gctgggatca gcaggctcgc atgggcggcg accagccgct
     4321 cggcctgggc gaggcgctcc aggcgatcga gcggctcggc gactacgaag agctcgagcg
     4381 caccttgcgc ggcgactatc ccggcgcttc tttggaagac gtcgacctgg accaggtgcg
     4441 ccgcaccatc ggcgaggacg ccgcgcgcga tctggaaaag cttcgccgcg tcgaacgcgc
     4501 gctggaagag gccggcgtcg tcgcgcggcg cgacggcgac tggcagctga cgccgcgcgg
     4561 cattcgcgcg ctcggccagc gggcgctcgc aaccgtgttt tcgaagctgc gcgacgatcg
     4621 cgcgggcaag cattacgcgc cgcagaccgg cgcggggggc gagccgaccg gcgcgacccg
     4681 cacctggcgc ttcggcgacc atttccggat cgacgtgcgc cgcagcgtcc agaacgcggt
     4741 gctgcgcgaa ggcgtcacgc acggtcccgt gcacgtcacc gcgaaagact tcgagatcgc
     4801 cgagatggag acgcgcacgc gcaacgcgac cgtgctgctg ctggacatgt cgttctcgat
     4861 gccgatgcgc ggcaactggg ccggcgcgaa acgcatggcg ctggcgctgc acgcgctcat
     4921 cagcagccag tatcccgacg atcgcctgga tatcgtcggc ttcagcgact acgcgcgcgt
     4981 catgtcaccc gaagatctgg ccaacgtcgg ctgggagcac gtctacggca ccaacatgga
     5041 gcacgccttc aacctggccg gacggctgct gtcgaagtat ccgggctcga acaagcaggt
     5101 gctgctggtc accgacggcg agccgaccgc gcacatcatc gacggcaccg atcaagtctt
     5161 cttccactgg ccgcccgtgc gcgagacgct tgagaagacc tacaaggaag cgc
//
LOCUS       DATLFO010000374        32565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_147626, whole genome shotgun sequence.
ACCESSION   DATLFO010000374 DATLFO010000000
VERSION     DATLFO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 32565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147626"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..580)
                     /locus_tag="VM841_15265"
     CDS             complement(68..580)
                     /locus_tag="VM841_15265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVL91580.1"
                     /translation="MSSKTPWEMPPPVEGDEMRPLPSHYQYCFGCGNDHPTGLHLQMS
                     GGGNRVEGHFVVTEHHQGAPGLAHGGVIAAAMDEGMGFVLYLLATPAVTARIEVDFRR
                     PVPVGSTLELLGVIDQVEGRKIWVRMTGSLDGEVAVRSQALFLKVGVEHFLPHAKKFG
                     DEHMERPYNP"
     gene            639..1493
                     /gene="mca"
                     /locus_tag="VM841_15270"
     CDS             639..1493
                     /gene="mca"
                     /locus_tag="VM841_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HVL91581.1"
                     /translation="MDLCLLQVHAHPDDEASKAAATAARYAAEGVRTVLVTCTGGEAG
                     EILNPALDTPEVRANLPAVRMRELEEAVAALKYSATHLLGYRDSGMPGTEANADPRNF
                     WNAPVEEATERLVRIIREERPQVIVAYSDDQETYPHPDHLRVHDIAILAFDAAGDPAR
                     FPDAGEPFAPLKLYYCGFTMRALKAAHAALLASGRESPYEEWLEKRKDTDHRFTTRID
                     VGGYLAARAQALAAHATQCPPDSFWFTLTPDEMREIYPWEEFIRARSRVPVPEGETET
                     DLFHGIRG"
     gene            1514..2290
                     /locus_tag="VM841_15275"
     CDS             1514..2290
                     /locus_tag="VM841_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL91582.1"
                     /translation="MAELFGIEGKVALVTGGSRGIGLMIARGFVEAGAKVYISSRKAD
                     ALEQAAAELSGTGECVAIPADLSTEDGCNELAAAVAGREPAVHILVNNAGATWGAPIA
                     EYPASAWDRVIDTNVKGPFFLTRALLPQLRTAATADDPARVIMIGSIDGIRISEFEHY
                     AYPASKAAIHMLARDLAHKLVRDNITVNAVAPGLFPSKMTNFLFSSDHAGEVIAQTVP
                     MGRPGRPEDMAGIAIFLSSRAGSYLTGAVIPVDGGVSTHG"
     gene            2366..3760
                     /locus_tag="VM841_15280"
     CDS             2366..3760
                     /locus_tag="VM841_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91583.1"
                     /translation="MLRKIAGALAALSLLMPASGGAVDVPAGPSSDNVRHIRNIPAGT
                     GVGGVFAGTHYYLSAARSGSFLPGGAGTGGILAFDATNARNPVLVGKLPLPHSQNEDL
                     EVSEKRNLLLVVQDRRKSVSAVATSPIIGGILFVIDISNRNQPVLRSTLQLPAQVATR
                     DDGAPLGGPGHTASCIQDCNYAWISGSRDHGVHVVDLRDPSSPAWMGVIKGPAGGNNA
                     IYDPGTVHDVHVDRFGEVWIAGSGGTALYRMTKNPLKPQFIAAVNKRDNERFNGLIHH
                     GALRYTKDLVLVGEEDYENTCGSADGKRQDGSLQVWRIDRKAKRLVPVSSWDVTITDA
                     TMRMLAEEVGITCSSHWFDVNKYSIVADGWYEMGVQFIDISKPAKPRHVGWFIGTNSA
                     ASQARFHPSDSGVVYVADYNRGLDVIEIDNSGRKAKSVSMSAGAVRAAHAGVRFPLEP
                     DPEFGFACLRRAGV"
     gene            3846..4742
                     /locus_tag="VM841_15285"
     CDS             3846..4742
                     /locus_tag="VM841_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HVL91584.1"
                     /translation="MDVAVAGSSGLIGRALARELESAGHRVRPLLRPSSPPHPDGIRW
                     DPAGGWIDEDSLRGAGAIVNLAGEPIGARRWTVRHKALIRQSRIDATSVLARAIAAMP
                     DPKPAFVSSSASGYYGERGEEILEEASEPGEGFLARVCVAWEAATRPAADAGARVAIS
                     RSGIVLDPGGGLLQRLVPLFKLGLGGRLGSGRQWTPWISLRDEARALAFLIANDDVSG
                     PFNLCTAEPVRNQELTRSIGRAVRRPTPFVVPAFALRAAFGEDAVRDAFLTSARLMPA
                     ALLKAGFSFEDPALGAALASML"
     gene            complement(4739..5173)
                     /locus_tag="VM841_15290"
     CDS             complement(4739..5173)
                     /locus_tag="VM841_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91585.1"
                     /translation="MQIRHGMRSDIRGGHEENRAPAANPLTMKRLVLASMLLLATAVP
                     SGAHAAVTRCQGLAPMESGCSFTVIATTPHVSQLLTSNLFTGIAEYEVTGPSGRFAGS
                     CYAHPGVVTCIPSERGWFDPGDEIRVSVSVTGAGDWEFSLWH"
     gene            5302..6726
                     /locus_tag="VM841_15295"
     CDS             5302..6726
                     /locus_tag="VM841_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91586.1"
                     /translation="MLRARHRGRRTAALLCLASLSIWLRPAAAAPSSDLRAIVRADAD
                     WLMQAVTPDGGIGTWIDRAWLVPYRSNFAAIGLARATAVTGDPRYAAAAWRWLEWYAN
                     HMDARGFVTDYQWAGGGWRSTGDMDSTDAYAATFLLAAHETLRATGDLTRLSGLRGAL
                     HRALDALDATATGEGLHFAKPTYPIKYLMDEAENYAGLNAAVALGAALQDAALASRAR
                     RDAERLKAAVPLFKHPVAGYHWALDAMNRPAAALNWNELYASAVAQAWAIAFGLVEGA
                     EAASLFARFEAAQPRWDEPLATTQYPWGPGRVDYWPVVALAAKRAGDPARAAQAATRI
                     RDAALSENRFWPYTTAEAGEMIMVMTDGITQPAPLVPIATVVLAPPPAADPSRGVGAT
                     LTERVSGRPISGATVRFHAIDAISGARGGLLCEATTNTAGLAECGGTAARIAAIRGGG
                     FEATFGGSAIYAPGEARRSTLVIR"
     gene            complement(6716..7486)
                     /locus_tag="VM841_15300"
     CDS             complement(6716..7486)
                     /locus_tag="VM841_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HVL91587.1"
                     /translation="MPEGDTIFKAAQRFREALAGKRVVRLAIPVHGAGAPAADVAVDA
                     SVTTVESHGKHLWIAFDDGRALHVHLGMHGVAHVVGPGSAVPKSARAVIDVGDAIAIC
                     RRAPVVELLDARRMTPRHLGPDLLDPAPDIDEAVRRARLLDPSTPVGVALLDQRVAAG
                     IGNVYKSEVLFARRVDPFARIGDLTDGTLGALLRTAAAMLAANVGPGRRTTVPGGLAV
                     YRRAGRACLRCRTLIRSQRLGEHARSTYWCPACQTVSE"
     gene            complement(7488..8270)
                     /locus_tag="VM841_15305"
     CDS             complement(7488..8270)
                     /locus_tag="VM841_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVL91588.1"
                     /translation="MTNAQTRWREALASWAIPDEILAEAPEEPWGLPVSLFAAPETPA
                     DTPSRRRALEALAGGGDVIDVGAGGGSASLSLLPAVRRIVAVDSSAAMVASFAEAAAV
                     AGVAHQEIEGAWPDVAPEAGRADVVVCHHVFYNAPALTPFVRALTAAARRRVVVEITA
                     EHPRARTNPLWERFHGLERPARPTADDALAVLRENGIEFESERFVQPQREQRMSEQER
                     VAFVRRTLCLPAQRDAEIAEAMREPGFDAPRELVTVWWDGTR"
     gene            complement(8348..12724)
                     /locus_tag="VM841_15310"
     CDS             complement(8348..12724)
                     /locus_tag="VM841_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVL91589.1"
                     /translation="MSLDAFSPAARAWFTSTFAEPTTAQTLGWEAISGGAHALICAPT
                     GSGKTLAAFLWALDRMATEPVPDKQARTRVLYISPLRALAVDVEKNLRAPLAGMALAA
                     ERLGETIHVPEAGVRTGDTPSRERAAMLRNPPDILITTPESLYLMLTSQARETLRSVR
                     WVIVDEIHALAPVKRGAHMALSLERLEEITGVTPQRIGLSATQRPLEEIASFLGGATE
                     TGPRPVTIVDAGIRKQLDISVVVPVEDMSDLSTVPPEDGTPMLGEAQTRRSIWPAVHP
                     KLLELVLAHRTTLIFANARRLAERLAARLNEMAEADIAKAHHGSLAREQRTIIEDDLK
                     AGRLRAIVATSSLELGIDMGSVDLVVQVESPGSVARGIQRIGRAGHQVGEPSRGVFFP
                     KFRGDLLEMAVVVQRMRDGEIEHTRYLRNPLDVLAQQIVAMCSVEDRGDAELLALVRR
                     SAPFAGLGEETYKAVLDLLSGRYPSEEFSGLRQRIVWDRRTGALRAREGAKKIAVISG
                     GTIPDRGLYGVFTPDGGRVGELDEEMVYESRPGETFVLGASTWRIEDITPSKVIVTPA
                     PGEPGKMPFWKGDRPGRPVELGRALGEFVRTTGEAKKPEQLLRERGLDEWAASNLLRY
                     LEDQRAATGAVPDDRTIVVERFRDELGDWRMCILSPFGARVHAPWAIALEERLERRTG
                     INVAAMWSDDGIVVRLGEAEEHVPLEDLLIPPEEIEELVLARLPGTPLFAATFREAAA
                     RALLLPRKMPGTRTPLWQQRQRAADLLSVAGRYPTFPILLEATREIVQDVFDVPALRG
                     LLGDIRARRIRVTTVETQQSSPFAQSLLFNWIAVYMYEGDAPLAETRAAALALDAGLL
                     RELLGSEDLRELIDADALESLEFELQRLDGVRPPKDADTTHDLLRFVGDLSEAELAAR
                     GARREWIDELLTQRRAIVAGVAGEQRLVAAEDASRYRDALGTALPQGLPAAFTDPVER
                     PLHDLVARYARTHGPFHHAEAAARLGTGAERVHAALIDLESEGRVLRGEFRPGGSERE
                     WCDADVLRTLKRRSLAALRKEIEPVDATAFGLFLPAWHGVGMHRRGPDALLETIAALQ
                     GAPIPSSILEDAVLRVRVDGYRPADLDALCASGDLVWVGAGPLGPSDGRVSLLFRDAV
                     RLLAPPPPDEPPAGAVHDAIRAHLAARGASFWPELLAASGVADGEAVLGALWDLVWAG
                     EITNDTMAPLRALRAGKRKASPQRGRPHPGRLRRAGPPAAAGRWSLVAGLRAPEASAT
                     EIAHARALQLLERHGVLTREAVRGEGLPGGFAGIYPVLKTLEESGRARRGHFVAGLGA
                     AQFALPGAVDRLRGVRAPLREDDAASVVVLAAADPAQPYGAALPWPETPHRPARAAGA
                     WVIVVNGEAAAYLERGGHSLVVFGDGADWVDGLASLVKDGYLSRLEISKIDGAPARES
                     SHAETLRSAGFADAYRGLTLRG"
     gene            complement(12818..13456)
                     /locus_tag="VM841_15315"
     CDS             complement(12818..13456)
                     /locus_tag="VM841_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91590.1"
                     /translation="MKAQGHRAAIAVVLALGALFATVAGTAQADVVVGGGTVVVKQQG
                     QGPAADNGTLVCEPGTGIGTGGACLDFAPGGFVEVLDALNGYNVAFQVCIDANGDGVC
                     VDDPKNQSPCRDRMFFSHDDAGQFHNPLGPLPSSMPCGNTASGWNGYVVFLCEGVHVA
                     QNGSSVGGAPHTHETTVANVSVTGPMASSGFGDFCGTAGRQEPVRKPYTIVR"
     gene            complement(13564..13890)
                     /locus_tag="VM841_15320"
     CDS             complement(13564..13890)
                     /locus_tag="VM841_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVL91591.1"
                     /translation="MKKTLPVAECCPVLDAPLTRREAERAASAFKVIADPARLRLLSL
                     IAAQPGREACTCDLVVPLGLTQPTVTHHLRVLTQAGLLERERRGTWTFYRVVPSALKT
                     LREILS"
     gene            14017..14412
                     /locus_tag="VM841_15325"
     CDS             14017..14412
                     /locus_tag="VM841_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HVL91592.1"
                     /translation="MGRVQLAINVKDRDEAITFYSKLFGAEPAKVKPGYANFTIADPP
                     LKLVLIENPDAPGTLNHLGVEVGSPEEVAAATARLAGAGLATTSEHAACCYAEQDKVW
                     VADPGGAAWEVYTVISDAEVMAPSVSGCC"
     gene            14456..15028
                     /locus_tag="VM841_15330"
     CDS             14456..15028
                     /locus_tag="VM841_15330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HVL91593.1"
                     /translation="MSISPVEAVRAEAERAIALGRSLDPTEWNAASRCEGWTVADVFG
                     HLAGDCDRFREWLADAERGVLVPPFPPEQFRADNAMLLEQLTGTTGPQRLAAFETACD
                     RWLERAEALDPTTPQRHPRGTITVGQQLWLAAGEFAIHGWDVAAALGRRWDPPEALND
                     IVAAWSSVVAPVREGEPWIALLRSTGRAQV"
     gene            complement(15056..16576)
                     /locus_tag="VM841_15335"
     CDS             complement(15056..16576)
                     /locus_tag="VM841_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVL91594.1"
                     /translation="MVVERFDAIVVGAGHNGLVAAAYLARAGRRVLVLERRDVTGGAA
                     VTEDLAPGFRVSTASYSLSLLRPDVHAELDLARHGLVITPKDPQMFVPLPDGRHYFVW
                     RDGDRTREEIARINPADADALGRWGAFWTEAVAKIRPLVEAPDPPGLDGVRAALGEEL
                     YRMAVAGSCADTVRAFFDAPEIQGPMAGQGIIGTKLGPEEEGTAWIMTFHAIGGELNG
                     MDGTWAYVRGGMGGVTQAIAAAAREAGAEIRVSSPVSRIVTGGGRVRGVTLADGTMIE
                     APLVLSNATPAVTFGTLLDDGVLPPSFAERVASWQYEGAVVKVNLALRELPSFSMLPG
                     TEPAPQHLGTVEIAHSIEYIQQAREDARGAMPSSKPFMEVFLQSSGDRTLVPGGEGHV
                     LSAFTQYAPEQPVDWDAARRAARDNAVAALAAYAPNLPDAIVTEDVLAPPDLEERLAL
                     TGGDIFHGALSPEQSFGERFDYRTPVEGLYLCGSGARPGGGVMGAAGRNAARVALG"
     gene            complement(16567..17580)
                     /locus_tag="VM841_15340"
     CDS             complement(16567..17580)
                     /locus_tag="VM841_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006038606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVL91595.1"
                     /translation="MSAQPRPAPHPGDVTSMTIVVMSSSNAIAGTLREAGFTAVATRR
                     SGGHVPAALTIVDEPPDRAVDALRALGDAPALAVAATPADSARLLDAGARDTIADPSD
                     RATLVARVRASLELVALRRLLTDTGALLEEKVRMRTRELEDARREVLDRLVRAAEYRD
                     DDTAEHTRRVGETSALLAAAAGLDAGECETIRLAAPLHDIGKIGVPDSTLLKPGPLEA
                     DEFERIRAHTVIGGRILWGSHAVVLKAAQEIALFHHERWDGAGYPAGFAGEDIPLSAR
                     IVAIADVFDALTHERPYKKRWTVEQSLAEIERLAGNHLDPTLAAAFVTLIRQGALETI
                     EWS"
     gene            17720..17974
                     /locus_tag="VM841_15345"
     CDS             17720..17974
                     /locus_tag="VM841_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91596.1"
                     /translation="MARRLGLLIVGLLVLGGAMPANAGPDAAAAEIVRGSARAGFERP
                     ADVPWLLIAGLLVAAILTLQAGLRSQPRAVRVSIRPPKQR"
     gene            complement(18034..18582)
                     /locus_tag="VM841_15350"
     CDS             complement(18034..18582)
                     /locus_tag="VM841_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91597.1"
                     /translation="MRRMICVAVAAGALWVAAVPAAMAGGGGCRTLPQSGSGSTVVMR
                     ASCFTPAVLWVDPGATVRFVNEDAIAHHVFGWPSLGLQSPDLRAGMSYEFTFTRAGVY
                     PFACWLHPGMTGVVVAGSPLPSPGAIGTLGSSGEVLSPEETRAAARTAGSSAGAAGGA
                     AALAGGVLGTAGYVIGRRRSQA"
     gene            complement(18642..19865)
                     /locus_tag="VM841_15355"
     CDS             complement(18642..19865)
                     /locus_tag="VM841_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVL91598.1"
                     /translation="MRHEDARTLASARADGELEAAGIAELEAHLAGCDECRAFTHAIP
                     RLGALARALPEPALRDGFAVPSMSTRRTRRIRRPRLAGWAAAAVVAAIALLVSGLPAG
                     GFRVEPAGAAEALLTIRTLDVTREIVETSFDGTRTTTIERIRYRAPATTRIDRTVTDA
                     RGTATQTEIRLPGARALIQGSAATLQTGLPPDPNMVPEPLSPSLALAGRVTGPGPQVA
                     GRATERVVLESPDGTVVREALIDASQASLLSSEVAVVLRKQAAGARRTVAKRVTAIAY
                     NVTLDDSLFALPTVPAGSGGFRPQPPASLSMPPAATPDGFSVVHAGSGPAGDAVLYAR
                     GAFTILVQTGPRAETEDDTVVTRPARAGDGEAWYLIGLYDLPALRFRSGGTTITISAP
                     MTREQLEAVAARMYP"
     gene            complement(19868..20398)
                     /locus_tag="VM841_15360"
     CDS             complement(19868..20398)
                     /locus_tag="VM841_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91599.1"
                     /translation="MERTAGDGIGRDEAAFAALVHRHAGAVFGFVLRRVRDRALAEDL
                     TQETLLRAWRGRETYRGGDERMRGWLLAIAGNVVRDQARMRARRVAESPADAAGDLAA
                     EGSDPAVRHEHAESIQRLRIALAALPENHREMFLLRERDGLSYKEIAEVLGCPVGSVM
                     SGLARARERLMGAVEG"
     gene            complement(20486..21136)
                     /locus_tag="VM841_15365"
     CDS             complement(20486..21136)
                     /locus_tag="VM841_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVL91600.1"
                     /translation="MTPPKTVKPKRAEYLGPARRRPLVLDAALELFAQGGFADASMEA
                     LARLAGTTKPVLYDCFSGGKQEIYFALLEREEERFRAHMMGVLATTGRMKLRDALTSG
                     LSAFLDYADVNPSAFRVMFGPPGTSDPGIVERGVRVREQIITIMTDRADALAEPVNAP
                     PVVVELYNRAIVAVAEELARWWLAAKPMERDKMVAVAVTWMMKGFEGILPAEWTRA"
     gene            21412..23313
                     /locus_tag="VM841_15370"
     CDS             21412..23313
                     /locus_tag="VM841_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91601.1"
                     /translation="MIRWKRSWEDLSEALPLDVMPCSNGEFYPPPPTKEQKAIMELAD
                     REVERVRKKFNMSRRDFVRTTAAMAICFWAKDTIYGSARAHNTATTEACDLQWDEAVG
                     DMTLNNLPGEFIFDVQSHHVESDGMWRVTNPGFHAFFAGVWSQAGLLGSAPGYRPGGN
                     LDPGSGNFSVRGAGAGELDPIENLSRYHYLKELFLDSATTMTVLSAVPAAPDISQPLP
                     IERAALTVQMVNELAGSERTVMHAFVMPNRGSAGTVASSLGKEPVYMQREFEIMEERA
                     VKWGSIIRGWKTYPAWGDIPYASGWFFDDELVGQRFIDHVRYISQKYPNVRPNIATHK
                     GFALPAFDQRAASPRDIGPAASRNPDVNFIVYHSGFDGETMTSYGGQATDPTNIAGDG
                     PKGDARVSSADRTVDSLIKSLRENNWDATRFGAIPGQKWANIPNVFAEIGSTWRTVTG
                     RGPVQEAHLLGKLITYVGPKRIAWGTDSLWFGSPHAEIIAMRRVSYSKAAKVWDFYGL
                     PYAIDGDADDPTVDTRNPISYSPSHPNAGVIPGWPTDGTSHPERSIRNWIFGRAAAEV
                     YGVDADKHLNDRANHPISCDNVQKIRDAYILNQYTPKEQVPMASNALIGARTPQELKI
                     ERMNQPWAP"
     gene            23301..23834
                     /locus_tag="VM841_15375"
     CDS             23301..23834
                     /locus_tag="VM841_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91602.1"
                     /translation="MGAVIRRTTVLIALLALLVPTAARASTTTYGPPYGNGPSGGDTY
                     NRIDRNGSTGEMSILRYNPVPANGTLGCAGNGGWATFTLPHTAASDIVSVTLSFRGAI
                     VDPYSFMSLSVYRDDHFVDTHIERGPILGDGTIVLEPRTAASGEVTLIFGLQAGSSCP
                     NVQLARAVFDSVIVVEA"
     gene            complement(23852..24235)
                     /locus_tag="VM841_15380"
     CDS             complement(23852..24235)
                     /locus_tag="VM841_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91603.1"
                     /translation="MRRTLAMLLALSGLTGAHASPAEPALITAAALRFVPGDDALPLG
                     PLIVEQGRRLLFVNADNLGVHSIYSVEYDEDGWPLFWTDQQNLGSAAEVEGVPDLLPR
                     AYAFFCSNHPEMKGTLVVTDDDQGA"
     gene            24324..24866
                     /locus_tag="VM841_15385"
     CDS             24324..24866
                     /locus_tag="VM841_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91604.1"
                     /translation="MGLWPSLVVVEDVATGEPVEAGRIEGRRLELLYRAHATKAARTA
                     YLLTGDAALADDLVQEAFVRLAGRFVDWRREDGFEPYLRRTIVNLFRTRLRRIERERE
                     RHRRVAASPVAAGGDLADLIAVRDALMRLSERQRAAVVLRYYEDLPEAEIADLIGCRP
                     GTVKSLLSRAMDALRAELSR"
     gene            24872..26374
                     /locus_tag="VM841_15390"
     CDS             24872..26374
                     /locus_tag="VM841_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91605.1"
                     /translation="MDVKQLLSDLAERQMPSAPPLRPGTVQRAHRRRAMVAAATSLAV
                     VAGALGVWAGIGSLSRPRPVPPAERDASFSMIALDGTEATALAAHGDAAWAATDGKLY
                     RVAGSEPERIGPFPDTYLGQPFEQRMQIDDLAANENGVWVAASPVGARCGESDAVAKD
                     PCGDARWIPAGGAPQPARFFAGSRGGIAIGNGSTWVGGTTVMYKVTTDQRDAPVVAPR
                     KGVRSIVGIASTLGQVFALVEPDDGRVDRALLSIEPNEGGGLGRLDLPGALIVRAGLG
                     SLWVAAWHEGAAMLYQIHPERFSILRTIRLIESPGDAAKVLIKGLAFSGSTVYAAAAR
                     GDQGIVLAFDARTGTVETLRAAGVPVDIAAGHEALWVLHSRPARLARMTIPDPWDAHA
                     PINDDERAEAVPERPGLEHPRITRIDRLEPSGDRLTIGFLGGDYPCGMLDHVELWEDR
                     GRLMVLVVEGVRPTVRDGQWGCKSVGIGRTTTVEIPERLRGLEPADAAQD"
     gene            26435..27619
                     /locus_tag="VM841_15395"
     CDS             26435..27619
                     /locus_tag="VM841_15395"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVL91606.1"
                     /translation="MLPIREVDASIAELRDVVGRVQRAGAVRDVSFIVELLRAADRLT
                     ATALTAIARDKLDEGFGLPPAIVLQMDARRTSWEARHLLRAATTLAAMPAATSAAEMG
                     LLSISQLRAIVAAARTCDAAGRARIDESIADAVVVHADADPDAIVAAVDDQAATLRRD
                     RTERREHRAVEAGFVAIQPTLDGRASLYAEGDAESMATIADALDRQAGPPRDDRTRAQ
                     QCFDALVGLCAAGPAGDRRRPRVLATVDLAALTRSERDAAARLLWSLSGGSPRLSPIA
                     TCTLLCDADVLPIIFERGQPVAIGDATSPIPAKIRRAVAARDQGCRFPGCGSPLAWAD
                     AHHITPRHRGGRSDPDNLVMLCRRCHRRVHGHGWIVSLRAGGDIEFRHRGRRYRSPPP
                     AG"
     gene            27662..29440
                     /locus_tag="VM841_15400"
     CDS             27662..29440
                     /locus_tag="VM841_15400"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HVL91607.1"
                     /translation="MAAKTPPYETPLRESDLDRARLHAPFPRSAFVAIGAWVAWFALI
                     LSPLTGVHCGGGDATQCTPLVNDLLLPATSVFGIASLAGLALRRSWAGITSSAGAATG
                     LWMGILVIGDAPPLVAGEAAIFALAVVGSLMTAAHNRGILTLAGDEPADPPPAESLRP
                     LGALVRRRIALAVALLLAGGIAIAIVKSNERNARRFEATAPQTTATVVDGGDPLRVRY
                     RHPVSGRIVEAEIPNLLEVYERGERVRIRYDAGDPGRVGVVAEPYDAESPLILFGFLP
                     LAGTGALAASAWWVRRLHMIAGRPGRRVRTRIDATEIDAGEQDPPSGIVVVTVFPVDG
                     TEPVATFETLPGGIPSDLPYDGPAEARGIIGWHAPLVVRVGDLWLWPRRRVRVGRALA
                     RLLSKVEETLQHERDAKAAAEAANEIRPVEAAPDPGWHPVRLFRRRSHLTLLESMRKQ
                     TLVSVIVNAGLAQWGGVMMLTGRPSRVAGIVAVVAGLAWADPADATPAELYRLIDFRS
                     TIAGFVSSVPAAAVALTFFFFGAGPEALAIAGPAGLLCDALVAPTRSWLARIQEALDE
                     QGFPLSVAAVVSAPAVEIRRWRQPRE"
     gene            complement(29519..32179)
                     /gene="acnA"
                     /locus_tag="VM841_15405"
     CDS             complement(29519..32179)
                     /gene="acnA"
                     /locus_tag="VM841_15405"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HVL91608.1"
                     /translation="MSLNDPLGALDRLQAAGGSVALYRLDRVLDAGSIARLPFVVRIF
                     LENVLRHQGTDAERLHLDALAGWPGDAPVEFPFLPGRVVLQDFTGVPVVADLAAIRGA
                     VKRLGGDTSRVNPRVPVDLVIDHSVIVDFFGSRDAFARNVDLEYERNGERYAFLRWAQ
                     KSFRKMRVVPPGSGIVHQVNLEFLADVVMTDENGVAYPDTLVGTDSHTTMIGGLGVLG
                     WGVGGIEAEAAMLGEPVTMLTPSVVGVRLDGSMPAGATATDLVLTLTEMLRAHGVVGK
                     FVEFYGPGLSALTVADRATIANMSPEYGATEGFFPVDDQTLAYMRATGRSDDLIDLVE
                     RYCKEQHLWHDHGAAPLFGELLELDLSTIEPSLAGPRRPQDRVVVGEVGSTFRRELAT
                     LRGESGEPAIKKIPVTTERGDTEVADGTVAIAAITSCTNTSNPSVMIGAGLLARNAVA
                     RGLRVDPGVKTSLAPGSPVVLDYLTRAGLLDDLEKIGFYAVGFGCTTCIGNSGPLLDP
                     IADAIDENALAVSAVLSGNRNFEGRIHPQVRFSFLASPPLVVAYALAGTVNIDLNNDP
                     LGTGTDGKPVFLRDIWPSDQEIASTVASAMNPESYRSRIGGLFDGDDRWKSMPVPEGD
                     LYEWDPSSTYITEPPFVSSIGMEPPPVTDIAGARCLVFVGDSVTTDHISPAGSIAKDS
                     PAARWLGERGVAPGKLHSYGARRGHHEVMMRGTFANIRLRNLLVPGSEGGVTAHLPGG
                     ETMTIFDASVKYRAAGVPLVILAGKEYGSGSSRDWAAKGTTLLGARAVIAESFERIHR
                     SNLAQMGVLPLEFAPGETASSLGLDGRETFDITGLSDLKPGQTLRVVARRDDGTTVEF
                     DTRCRLDTPTEVRYFREGGILPAVVRELAR"
     gene            complement(32259..>32565)
                     /locus_tag="VM841_15410"
     CDS             complement(32259..>32565)
                     /locus_tag="VM841_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91609.1"
                     /translation="PVREPGSVPAGPRPRPDGTDPGSRTGERRGPAPAPDGTQKTPAA
                     SSPGSNPAGAAAGPRVRPFESPETGALAAVAPIAGGVAALGLVFAVLVLGMRAAHRP"
BASE COUNT         4761 a        11622 c        11431 g         4751 t
ORIGIN      
        1 cccggcgagc tcggccgggc cgactgccgg ctgatcaacc agcccccgag ctagaccgag
       61 gccggcgcta cgggttgtag gggcgctcca tgtgctcgtc gccgaacttc ttggcgtggg
      121 ggaggaaatg ctcgacgccg accttcagga acagggcctg cgaccggacc gccacctccc
      181 cgtcgaggct gccggtcatg cggacccaga tcttgcggcc ttcgacctgg tcgatgaccc
      241 cgagcagctc gagcgtggag cccaccggga ccgggcggcg gaaatcgacc tcgatgcgcg
      301 cggtgaccgc cggcgtggcg agcaggtaga gcacgaagcc catgccttcg tccatcgcgg
      361 cggcgatgac gccgccgtgc gccaggcccg gcgccccctg atggtgctcg gtgacgacga
      421 agtgaccttc gacgcggttg ccgccgccgc tcatctgaag gtgcagtccg gtcggatgat
      481 cgttgccgca gccgaagcag tactggtagt gggagggaag cggccgcatc tcgtcgccct
      541 cgacgggcgg cggcatctcc cacggcgtct ttgagctcat cggcgcgcaa gcgtatcccg
      601 cgccgcgcgc acgcgcatct tggtaggctc cgcttcccat ggatctgtgc ttgctgcaag
      661 tgcacgcgca ccccgacgac gaggcctcca aggcggccgc gaccgccgca cgctacgcag
      721 ccgaaggcgt gcgcacggtc ctggtcacct gcaccggcgg cgaggccggc gagatcctca
      781 acccggcgct ggacacaccg gaggtgcgcg cgaacctgcc ggcggtgcgg atgcgcgagc
      841 tcgaggaggc cgtcgccgcg ctgaagtact cggccacgca tctgctcggc taccgcgaca
      901 gcggaatgcc cggcaccgag gcgaatgccg atccgcgcaa cttctggaac gcgcccgtcg
      961 aggaggccac cgagcggctc gtgcgcatca tccgcgagga gcgtccacag gtgatcgtcg
     1021 cctacagcga cgaccaagag acctatccgc atcccgacca tctgcgcgtg cacgacatcg
     1081 cgatcctggc gttcgacgcc gccggtgatc ccgcgcgctt ccccgatgcg ggcgagccgt
     1141 tcgccccgct caagctctac tactgcggct tcacgatgcg cgcgctcaag gccgcgcatg
     1201 cggcgctgct cgccagcggc cgtgagagcc cgtacgagga gtggctcgaa aagcgcaagg
     1261 acaccgatca ccgcttcacc acgcgcatcg acgtcggcgg ctacctggcc gcgcgtgcgc
     1321 aggcgctcgc cgcgcacgcc acgcaatgcc cgccggactc gttctggttc acgctcacgc
     1381 ccgacgagat gcgcgagatc tatccctggg aggagttcat ccgcgcgcgt tcgcgcgtgc
     1441 cggtgcccga gggcgagacc gagacggatc tgttccacgg aatccgaggc tgacgagggg
     1501 gagttcgatg gggatcgcgg agttgttcgg catcgaggga aaggttgcgc tcgtgacggg
     1561 cggctcgcgc ggcatcgggc tgatgatcgc acgcggcttc gtcgaggcgg gcgccaaggt
     1621 ctacatctcc tctcggaagg cggacgcgct cgagcaggcg gccgccgagc tgtccggaac
     1681 cggcgagtgc gtcgcgatcc ccgccgacct gtcgaccgag gacggctgca acgagctcgc
     1741 ggccgcggtc gccgggcgcg agcccgcggt gcatatcctg gtcaacaacg ccggcgcgac
     1801 gtggggtgcc ccgattgcgg agtacccggc gtcggcttgg gatcgggtca tcgacaccaa
     1861 cgtcaagggg ccgtttttcc tgacgcgagc cctgctgccg cagctgcgga cggccgcgac
     1921 cgccgacgat cccgcgcgcg tgatcatgat cggctcgatc gacgggatcc gaatcagcga
     1981 gttcgagcac tacgcctacc ccgcctccaa ggccgcgatc cacatgctcg ctcgcgacct
     2041 cgcacacaag ctggtgcgcg acaacatcac cgtcaacgcg gtcgccccgg gtctgtttcc
     2101 ctccaagatg accaacttcc tgttttcctc cgatcacgcc ggcgaggtga tcgcccagac
     2161 cgtgccgatg ggccggccgg gccggcccga ggacatggcg ggcatcgcga tcttcctgtc
     2221 ctcgcgggcc gggtcgtacc tgaccggcgc ggtgatcccg gtggacggcg gcgtctcgac
     2281 ccacggctag gaggacaagc cgcccgaaac agcgaataat gcatgttgcc cgccacgagc
     2341 gggcacgagc gagacggggg agaccgtgct gagaaagatc gctggggcgc tcgccgccct
     2401 ttcgctgctg atgcccgctt ccggcggggc cgtggacgtg cccgccgggc cgtcctccga
     2461 caacgtccgg catatccgca acatccccgc cggcaccggg gtcggcggtg tcttcgccgg
     2521 gacccactac tacttgagcg ccgcgcgctc cggctccttc ctgccgggcg gcgcgggaac
     2581 cggcggcatc ctcgcctttg acgccaccaa cgcccgcaac ccggtcctgg tcggcaagct
     2641 gccgctgccg cacagccaga acgaggacct cgaagtgtcg gagaagcgca acctgctgct
     2701 ggtcgtgcag gaccgtcgca agtccgtcag cgccgtggcc acctcgccga tcatcggcgg
     2761 catcttgttc gtcatcgaca tctcgaaccg caaccagccg gtgctgcgct cgaccttgca
     2821 gcttcccgcc caggtcgcca cccgtgacga cggcgccccg ctcggcggcc cgggccacac
     2881 ggcctcctgc atccaggact gcaactacgc gtggatcagc ggctcgcgcg atcacggcgt
     2941 acacgtcgtc gaccttcgcg acccgagcag cccggcctgg atgggcgtca tcaagggccc
     3001 cgccggcggc aacaacgcga tctacgatcc cggtaccgtg cacgacgtgc acgtggaccg
     3061 cttcggcgag gtgtggatcg ccggatccgg cggcaccgcg ctgtaccgca tgacgaagaa
     3121 cccgctcaag ccgcagttca tcgcggcggt gaacaagcgc gacaacgagc gattcaacgg
     3181 cctgatccac cacggcgctc tgcgctacac gaaggatctg gtcctggtcg gcgaggagga
     3241 ctacgaaaat acgtgcggca gtgccgacgg caagcggcag gacggctcgc tgcaagtctg
     3301 gcgcatcgac cgcaaggcga agcggctcgt tccggtctcg tcctgggacg tgactatcac
     3361 cgacgcgacg atgaggatgc tggccgagga ggtcggcatc acctgctcgt cgcattggtt
     3421 cgacgtcaac aaatactcga tcgtcgccga cggctggtac gagatgggcg tgcagttcat
     3481 cgacatctcc aagcccgcca aacctcgtca cgtcggatgg ttcatcggca ccaacagcgc
     3541 cgcgtcgcag gcgcgcttcc atccgagcga ctcgggcgtg gtctacgtcg ccgactacaa
     3601 ccgcggcctc gacgtgatcg agatcgacaa cagcggccgg aaggccaagt cggtgtcaat
     3661 gagcgccggc gcggtgcgcg ccgcgcacgc gggtgtccgc ttcccgctcg agccggatcc
     3721 cgaattcggc ttcgcctgcc tgcggcgcgc cggggtctag gcgctttcgc agtttgtcgg
     3781 cgcaggtccg gcgaagcggc tgcgatctcg cggccgcttt cgccgttgcc gagtaggatc
     3841 gaagcgtgga cgtcgccgtc gcgggatcga gcggcctgat cggccgggcg ctcgcgcgcg
     3901 agctcgaatc cgcggggcat cgcgtccgcc cgctgcttcg cccgtcctcg ccgccgcatc
     3961 ccgacgggat tcgttgggat cccgcgggcg gctggatcga cgaggactcg ctgcgcggcg
     4021 ccggcgcgat cgtgaacctg gccggcgagc cgatcggcgc gcgtcgctgg accgtccggc
     4081 acaaggcgct catccggcag agccgtatcg atgccacgtc cgtgctcgcg cgcgcgatcg
     4141 cggcgatgcc ggatccgaag cccgccttcg tttcgtcgtc ggcgtcgggc tattacggcg
     4201 agcgcggcga ggagatcttg gaagaggcga gcgagccggg cgagggcttc ctcgcgcgcg
     4261 tgtgcgtcgc gtgggaagcc gcgacccggc ccgcggccga cgccggcgcg cgcgtggcga
     4321 tctcgcgcag cggcatcgtg ctcgatcccg gcggcgggct gctgcagcgg ctggtgccgc
     4381 tgttcaagct cgggttgggc gggcggctcg gcagtggccg tcaatggacg ccgtggatct
     4441 cgctgcgcga tgaagcgcgc gcgctcgcgt tcctgatcgc aaacgacgat gtctccgggc
     4501 cgttcaacct gtgcacggcc gagcccgttc gcaaccagga gctgacccgc tcgatcggcc
     4561 gggcggtgag gcgtccgacg ccgttcgtcg ttcccgcgtt cgcgctgcgc gcggccttcg
     4621 gcgaggacgc ggttcgcgac gcgttcctga ccagtgcgcg actgatgccc gccgcgctgc
     4681 tcaaagcggg cttttccttc gaggatccgg ccctgggcgc cgccctcgcg tcaatgctct
     4741 agtgccacag tgagaattcc cagtcgccgg cgccggtcac cgagacgctg acgcgaatct
     4801 cgtcgcccgg gtcgaaccag ccgcgctccg acgggatgca cgtcacgacg cccgggtgcg
     4861 cgtagcacga gccggcgaag cggcccgacg gtccggtcac ctcgtactcg gcgatgccgg
     4921 tgaacaggtt cgacgtcagc agctgggaca cgtgcggcgt cgtggcgatg acggtgaacg
     4981 agcagccgct ctccatcggg gcgaggccct ggcagcgcgt gacggccgcg tgcgcacccg
     5041 acggcacggc cgtggccagc agcagcatcg aggcgagcac gaggcgcttc atggtcagcg
     5101 gattcgccgc gggggctcgg ttctcctcgt ggccgccgcg aatgtccgat ctcattccgt
     5161 ggcgtatctg cacctggctc tcctatcgcg caaaagcgac cttcgtgcgg cttgccgcgg
     5221 atgcggccgg ccggcgaaac gcgcgcggct cgcttccgtt ttgacggggc gttcgcacgt
     5281 cgcctcggtg ggagtattca gatgcttcga gcccgtcacc gggggcgccg cacggctgcg
     5341 ctgctgtgcc tcgcctcgct ttcgatctgg ctgcgtcccg cggccgccgc tccgtcctcc
     5401 gaccttcgcg ccatcgttcg cgccgacgcc gactggctga tgcaggccgt cacgcccgac
     5461 ggcggcatcg gcacctggat cgaccgtgca tggctggtgc cgtatcgctc gaacttcgcc
     5521 gcgatcgggc tggcccgcgc gaccgcggtc accggcgacc cgcgctacgc ggccgccgcg
     5581 tggcgctggc tcgagtggta cgcgaaccac atggatgcgc gcgggttcgt caccgactac
     5641 cagtgggccg gcggcggctg gcgctcgacc ggcgacatgg attcgacgga cgcctacgcg
     5701 gccaccttcc tgctcgccgc acacgagacg ctgcgcgcga cgggggatct gacgcggctg
     5761 tcgggcctgc gcggcgcgct gcaccgggcg ctcgacgcgc tcgatgcgac cgcgaccggc
     5821 gaggggcttc acttcgccaa gcccacgtat ccgatcaagt acctgatgga cgaggcagag
     5881 aactacgcgg ggctgaacgc ggcggtcgcg ctcggagcgg cgttgcagga cgcggcgctc
     5941 gcctcccgtg cgcgccgcga cgccgagcgg ctcaaggccg cggttcccct gttcaagcat
     6001 ccggtcgccg gctatcactg ggcgctggac gccatgaacc gtccggccgc cgcgttgaac
     6061 tggaacgagc tgtacgcgag tgccgtcgca caggcgtggg cgatcgcgtt cgggctggtc
     6121 gaaggcgccg aagccgcgag cctgttcgcg cggttcgagg ccgcgcagcc gcgctgggac
     6181 gagccgctgg ccacgacgca gtacccgtgg ggtccgggac gcgtggatta ctggccggtg
     6241 gtggcgctcg ccgccaagcg cgccggcgac ccggcccgcg cggcgcaggc cgcaacgcgt
     6301 atccgtgacg ccgcgctgtc ggagaaccgg ttctggccct acaccaccgc cgaagccggc
     6361 gagatgatca tggtgatgac cgacggtatc acgcagccgg cgccgctcgt tccgatcgcg
     6421 accgtggttc tcgcgccccc gcccgcggcg gacccctcac gcggggtcgg cgccacgctg
     6481 accgagcgcg tctcggggcg gccgatctcc ggcgccacgg tccgcttcca cgcgatcgat
     6541 gcgatcagcg gcgcgcgcgg cggcctgctg tgcgaggcga cgacgaacac ggccggtctc
     6601 gccgagtgcg gcggcaccgc tgcccgcatc gccgcgatcc gcggcggcgg cttcgaagcg
     6661 accttcggcg gcagcgcgat ctacgcaccc ggcgaggccc gccgctccac gctcgtcatt
     6721 cgctgacggt ctggcacgcc gggcaccagt aggtgctgcg tgcgtgctcg cccaggcgct
     6781 gcgagcgaat gagcgtgcgg catcgcaggc acgcgcggcc ggcccggcga tagacggcca
     6841 ggccgcccgg cacggtcgtg cggcgacccg gccccacgtt ggcggcgagc atcgcagccg
     6901 cggttcgcag cagcgcgccg agcgtgccgt cggtcagatc gccgatgcgc gcgaacggat
     6961 cgacgcgccg cgcgaacagc acctcgctct tgtagacgtt gccgatgccg gccgcgacac
     7021 gctggtcgag cagcgcgacc ccgaccggcg tggacgggtc cagcagccgc gcccggcgca
     7081 cggcctcgtc gatgtcgggc gcgggatcga gcaggtcggg gccgagatgc cgcggcgtca
     7141 tgcggcgcgc atcgaggagt tcgaccacgg gtgcgcgccg gcaaatcgcg atcgcgtcgc
     7201 cgacgtcgat gaccgcgcga gcgcttttcg gcactgcgct tccgggtccc acgacgtggg
     7261 cgacgccgtg catgcccagg tgcacgtgca acgcgcggcc gtcgtcgaag gcgatccaca
     7321 gatgcttgcc gtgcgactcg accgtggtca cacttgcatc gaccgccacg tcggcggccg
     7381 gggcgccggc gccgtggacg ggaatcgcga gccggacgac gcgctttccc gccagcgcct
     7441 cgcggaaccg ctgcgcggcc ttgaaaatgg tgtcgccttc cggcacctca gcgggttccg
     7501 tcccaccaga cggtcacgag ctctcgcgga gcgtcgaagc cgggctcgcg catcgcctcg
     7561 gcgatctcgg cgtcgcgctg ggcgggcagg cacagcgtgc gccgcacgaa cgcgacgcgc
     7621 tcctgctcgc tcatgcgctg ctcgcgctgc ggctgcacga agcgctcgga ctcgaactcg
     7681 atgccgttct cgcgcagcac ggccaaggcg tcatcggcgg tggggcgggc gggccgctcc
     7741 aggccgtgga accgttccca gagtgggtta gtgcgtgcgc ggggatgctc ggcggtgatc
     7801 tcgacgacga cccggcggcg cgcggccgcg gtcagcgcgc gcacgaacgg ggtcagcgcg
     7861 ggcgcgttat agaaaacgtg gtggcacacc accacgtcgg cgcgtcccgc ctcgggcgcg
     7921 acgtcgggcc acgccccttc gatctcctgg tgggcgacgc cggccacggc ggccgcctcc
     7981 gcgaacgagg cgaccatcgc ggccgaggag tccacggcca cgatgcgccg gacggcgggc
     8041 agcagcgaaa ggctcgcgct gccgccgcct gcgcccacgt cgatcacgtc gccgccgccg
     8101 gcgagcgcct cgagcgcccg acgccgcgag ggcgtgtcgg ccggcgtctc gggcgccgcg
     8161 aacagcgaca ccggcagccc ccacggctcc tcgggggcct cggcgaggat ctcgtcgggt
     8221 atcgcccacg aagcgagcgc ctcgcgccag cgggtctgtg cgttggtcat gcgccgaagc
     8281 ctacgcgaag gctcggtccg cttctacagc gcccggcgcg agccgctcgc tacgacgcga
     8341 cggcgcgtca gccgcgaagc gtgagcccgc ggtaggcgtc ggcgaagccg gcggatcgca
     8401 gcgtctcggc gtgtgaggat tcacgcgccg gcgcgccgtc gatcttcgag atctcgaggc
     8461 gcgacaggta gccgtccttg acgagcgagg cgaggccgtc cacccagtcc gcgccgtcgc
     8521 cgaacacgac caggctgtgg ccgccgcgtt ccaggtacgc agcggcctcg ccgttcacga
     8581 cgatcaccca cgcgccggcg gcgcgcgcgg ggcggtgcgg cgtctcgggc cacggcagcg
     8641 cggcgccgta cggctgggcc ggatcggccg cggccagcac cacgacgctc gcggcgtcgt
     8701 cctcgcgcag cggggcgcgc acgccacgca ggcgatccac ggcgcccggc agcgcgaact
     8761 gcgcggcgcc gagaccggcg acgaagtggc cgcggcgcgc gcggccggat tcttcgagcg
     8821 tcttgagcac cggatagatg ccggcgaatc cgccggggag cccttcgccg cgtacggcct
     8881 cgcgtgtgag cacgccgtgg cgctccagca gctgcagcgc gcgggcgtgg gcgatctcgg
     8941 tggcgctggc ctcgggtgcg cgaaggccgg ccacgagcga ccagcggccg gcggcggcgg
     9001 gcgggccggc gcgccgcaac cgcccgggat gcgggcggcc ccgttgcggg gacgccttac
     9061 gcttgccggc gcgcagtgca cgcagcggcg ccatcgtgtc gttggtgatc tcgccggccc
     9121 agaccagatc ccacagcgcg cccaggacgg cttcgccgtc ggcgacgccg gaggccgcca
     9181 gcaactcggg ccagaacgag gcgccgcgcg cggccagatg cgcgcggatt gcgtcgtgga
     9241 ccgcgccggc gggcggctcg tcgggcggcg gcggcgcgag cagccgcacc gcgtcgcgga
     9301 acagcagcga cacgcggccg tcgctcgggc cgagcggccc ggccccgacc cacaccagat
     9361 ctccggaggc gcacagcgcg tccaggtccg ccggccggta gccgtccacg cgcacgcgca
     9421 gcactgcgtc ctccaggatc gaggaaggaa tcggcgcgcc ttgcaacgcg gcgatcgtct
     9481 cgaggagcgc gtcgggaccg cggcgatgca tgcccacgcc gtgccatgcg ggaagaaaga
     9541 gcccgaacgc ggtcgcgtcc accggctcga tctctttgcg cagcgcggcc agcgagcgac
     9601 gcttgagcgt gcgaagcacg tcggcgtcgc accattcacg ctcggagccg cccggccgga
     9661 actcgccgcg cagcacgcgt ccttcggact ccagatcgat cagcgccgcg tgcacgcgct
     9721 ccgcgcccgt gcccaggcgc gcggcggcct cggcgtggtg gaacggtccg tgcgtgcgcg
     9781 cgtagcgagc gacgagatcg tgcagcggcc gctcgacggg atcggtgaag gccgccggca
     9841 gtccctgtgg gagcgctgtt ccgagcgcgt cgcggtagcg cgaggcgtct tcggccgcca
     9901 cgaggcgctg ctcgcccgcg acgccggcga cgatcgcgcg ccgctgtgtg agcagctcgt
     9961 cgatccactc gcgccgcgcg ccgcgcgccg cgagctcggc ctcggacagg tcgccgacga
    10021 agcggagcag gtcgtgggtg gtgtcggcgt ccttgggcgg gcggacgccg tcgagccgct
    10081 gcagctcaaa ctcgagcgac tccagcgcat cggcgtcgat gagctcgcgc agatcctcgc
    10141 tgccgagcag ctcgcgtagc aggccggcgt cgagcgccag cgcggccgcg cgcgtttccg
    10201 ccagcggcgc gtcgccctcg tacatgtaga ccgcgatcca gttgaacagc agcgactgcg
    10261 cgaacggcga ggactgctgg gtctcgacgg tggtgacgcg gatgcgtcgg gcgcggatgt
    10321 cgccgagcag gccgcgcagc gcgggcacgt cgaacacgtc ctgcacgatc tcgcgggtgg
    10381 cctcgagcag gattgggaag gtggggtagc ggccggcgac cgacagcagg tccgcggcgc
    10441 gctgtcgctg ctgccacaac ggcgtgcgcg tgccgggcat cttgcgcggc agcagcagcg
    10501 cgcgcgccgc ggcctcgcgg aacgtggcgg cgaacagcgg cgtgcccggc agccgggcca
    10561 ggaccagctc ctcgatctcc tcgggcggga tcagcaggtc ctccagcggc acgtgctcct
    10621 cggcttcgcc gaggcgcacc acgatgccgt cgtcgctcca catcgccgcg acgttgatcc
    10681 ccgtgcgccg ctcgagacgc tcttcgagcg cgatcgccca gggtgcgtgc acgcgggcgc
    10741 cgaacggcga caggatgcac atgcgccagt cgccgagctc gtcgcggaac cgctcgacca
    10801 cgatggtgcg gtcgtcgggg accgcgccgg tcgcggcgcg ctgatcctcg aggtatcgca
    10861 gcaggttcga cgcggcccac tcgtccaggc cgcgctcgcg cagcagctgc tcaggctttt
    10921 tcgcctcgcc ggtggtgcgg acgaactcgc cgagggcacg gccgagctcg accggacggc
    10981 cgggccggtc gcctttccag aacggcatct tgccgggctc gccgggtgcg ggcgtgacga
    11041 tgaccttcga gggcgtgatg tcctcgatcc gccaggtcga ggcaccgagc acgaacgtct
    11101 cgcccggccg cgactcgtag accatctcct cgtcgagctc gccgacgcgg ccgccgtcgg
    11161 gcgtgaagac tccgtagagt ccgcgatccg gaatcgtgcc gcccgagatc accgcgatct
    11221 tcttggcacc ttcccgtgcg cgcagcgcac cggtgcggcg gtcccacacg atgcgctgcc
    11281 gcagcccgga gaactcctcg ctggggtagc ggcccgacag caggtccagc accgccttgt
    11341 aggtttcctc cccgaggccg gcgaagggcg ccgagcgccg caccagcgcc agcagctccg
    11401 cgtcgccgcg gtcctcgacg ctgcacatcg caacgatctg ctgcgcgagc acgtcgagcg
    11461 ggttgcgcag gtagcgcgtg tgctcgatct cgccgtcgcg catgcgctgc acgacgacgg
    11521 ccatctcgag caggtcgccg cggaacttgg gaaagaacac gccgcggctc ggctccccga
    11581 cctgatgccc ggcgcggccg atgcgctgga tgccgcgggc gacgctgccg ggcgactcga
    11641 cctgcacgac gagatctacc gagcccatgt cgatgcccag ctccagcgac gaggtcgcca
    11701 cgatggcacg cagccgtccg gccttgaggt cgtcctcgat gatcgtacgc tgctcgcgcg
    11761 cgagcgagcc gtgatgcgct ttggcgatgt cggcctcggc catctcgttc aggcgcgcgg
    11821 ccaaacgctc cgcaagccgc cgtgcgttgg cgaaaatcag cgtggttcgg tgcgccagca
    11881 ccaactcgag cagctttgga tgcaccgccg gccagatgct gcgccgcgtc tgcgcttcgc
    11941 cgagcatcgg ggtgccgtcc tcgggcggca cggtggacag gtcgctcatg tcctcgaccg
    12001 gaacgaccac gctgatgtcg agctgcttgc ggatgcccgc gtccacgatg gtcaccggcc
    12061 gcgggccggt ctcggtcgcg ccgccgagaa aggacgcgat ctcctccagc ggccgctgcg
    12121 tggccgacag gccgatccgc tgcggcgtga cgccggtgat ctcttcgagc cgctcgagcg
    12181 acagcgccat gtgcgcgccg cgcttgaccg gcgcgagcgc gtggatctcg tccacgatca
    12241 cccagcgcac gcttcgcagc gtctcgcgcg cctgcgaggt cagcatcaga tacagcgact
    12301 ccggcgtggt gatcaggatg tcgggcgggt tgcgcagcat cgcggcccgc tcgcgtgagg
    12361 gcgtgtcgcc ggtgcggacg ccggcctcgg gcacgtggat ggtctcgccg agccgttcgg
    12421 ccgcgagcgc catgccggcg agcggcgcgc gcaggttctt ctcgacgtcc accgcgagcg
    12481 cgcgcagcgg cgagatgtag agcacgcgag tgcgggcctg cttgtcgggc acgggctcgg
    12541 tggccatccg gtccagcgcc cacaggaacg ccgcgagcgt ctttcccgag cccgtcggcg
    12601 cgcagatcaa cgcgtgcgcg ccgccgctga tcgcttccca cccgagggtc tgcgccgtcg
    12661 tcggctccgc gaacgtcgag gtgaaccacg cgcgcgcggc gggggaaaag gcatccaggc
    12721 tcacccggtc agggtaccgc ccgtgccggt acccggcacg gcgaatgcgg ggaaggaccg
    12781 cctgccgccc ggcggtccct ccccaagaag cgagccgtta tcgcacgatc gtgtagggct
    12841 tgcggaccgg ctcttgacgg ccggccgtcc cgcagaagtc gccgaagccg ctggaggcca
    12901 tcggcccggt gaccgacacg ttggcgacgg tcgtctcgtg ggtgtgcggg gcgccgccga
    12961 cggacgagcc gttctgggcg acgtgcacgc cctcgcacag gaacacgacg tagccgttcc
    13021 agccgctggc ggtgttgccg cagggcatcg acgagggcag cggacccagc gggttgtgga
    13081 actgcccggc gtcgtcgtgg ctgaagaaca ttcggtcgcg gcacgggctc tgattcttgg
    13141 gatcgtccac gcagacgccg tcgccgttgg cgtcgatgca gacctggaag gccacgttgt
    13201 agccgttgag cgcgtcgagc acctcgacga agccgcccgg cgcgaagtcc aaacaggcgc
    13261 cgccggtccc gatcccggtg ccgggctcgc acacgagcgt gccgttgtcg gccgccggcc
    13321 cctggccttg ctgcttgact actacggtgc cgccgcccac gacgacgtcc gcctgcgcgg
    13381 tgcccgcgac cgtggcgaag agcgctccga gcgccagcac gaccgcgatc gcggcgcgat
    13441 gtccctgtgc cttcatagcc gtcctccctc tccccgccgg cgagggttcg ccggcgccgt
    13501 ggtcaagcat gcgggacggc ggcgaggggc tccgtgcatt tctgcacaga gcgaacgggt
    13561 cgttcaggac aagatttcgc gaagcgtctt caaggccgag gggaccaccc tgtagaaggt
    13621 ccaggtcccg cggcgctcgc gctccagcag gccggcctgg gtcaggaccc gcaggtgatg
    13681 ggtgacggtg ggttgggtca ggccgagcgg cacgaccaaa tcgcaggtgc aggcctcccg
    13741 cccgggctgg gccgcgatca gggacagcag gcgcaggcgg gcgggatcgg cgatcacctt
    13801 gaaggccgag gcggcgcgct cggcctcgcg ccgggtcagc ggcgcgtcca ggaccgggca
    13861 gcattcggcg accggcagcg tcttcttcac ggaaagatat tgacagacat ctatatcctc
    13921 ggtcaagatg cagaccctaa gacatcgatg agcatcaata tagacacctg tggatatcga
    13981 tgtgggtcga tgtggtcgaa agtggaggag aaagacatgg gacgagtgca gctggcgatc
    14041 aacgtcaagg accgggacga ggcgatcacg ttctactcga agctgttcgg ggccgagccg
    14101 gccaaggtca agcccggcta cgcgaacttc acgatcgccg acccgcccct caagctggtg
    14161 ctgatcgaga acccggacgc gcccggcacg ctcaaccacc tcggcgtcga ggtgggctcg
    14221 cccgaggagg ttgccgccgc gaccgctcgt ttggccggcg cggggctggc cacgaccagc
    14281 gagcacgcgg cctgctgcta cgccgagcag gacaaggtct gggtcgccga tccgggtggt
    14341 gccgcttggg aggtctacac cgtcatcagc gatgccgagg tcatggctcc ctcggtctcg
    14401 ggctgctgct agcggctctc atgccgccgc cgtcgtctga tagaacggcg gcggcatgag
    14461 catttccccg gtcgaagccg tccgcgccga ggccgagcgc gcgatcgcgc tgggccggtc
    14521 cttggatccc accgaatgga acgcggcgag ccggtgcgag ggctggaccg tcgccgacgt
    14581 cttcggccac ctggccggag actgcgaccg gttccgggaa tggctcgccg acgccgaacg
    14641 cggcgtactc gtgccaccgt ttccgcccga gcagttccgg gccgacaacg ccatgctgct
    14701 cgaacagctc accggcacca ccgggccgca gcgcctggcc gccttcgaga ccgcgtgcga
    14761 ccgctggctc gagcgggcgg aggcgcttga ccccacgaca ccgcaacgtc acccgcgcgg
    14821 cacgatcacc gtcggccagc agctgtggct ggccgcgggc gagttcgcga tccacggttg
    14881 ggacgtcgcc gccgcgctcg gccgcaggtg ggacccgccg gaggcgctga acgacatcgt
    14941 cgccgcatgg tcctcggtgg tcgcgccggt tcgcgagggc gaaccgtgga tcgcgctgct
    15001 gcgcagcacc ggccgcgccc aggtgtgacg cggtgtgacg cggtgtgacg cggcgtcagc
    15061 cgagcgcgac gcgcgcggcg ttgcgaccgg ccgcacccat cacgccgcca cccggccgcg
    15121 cgcccgagcc gcacaggtac aggccctcga ccggcgtgcg gtagtcgaat cgctcaccga
    15181 aggactgctc gggcgacagc gcgccgtgga agatgtcacc gccggtgagc gcgagccgct
    15241 cctccaggtc cggcggggcg agcacgtctt cggtcacgat cgcatccggc aggttcggtg
    15301 cgtaggccgc cagcgccgcg accgcgttgt cgcgcgccgc gcgccgggcc gcgtcccagt
    15361 cgaccggctg ctcgggcgcg tactgcgtga aggcgctgag cacgtgccct tcgccgcccg
    15421 gcaccagcgt ccggtcgccg gacgattgca gaaacacctc catgaacggc ttcgacgacg
    15481 gcatcgcgcc gcgcgcgtcc tcgcgcgcct gctggatgta ttcgatcgag tgcgcgatct
    15541 cgacggtgcc cagatgctgc ggcgccggct cggtgcccgg cagcatcgaa aacgacggca
    15601 gctcgcgcag cgccaggttc accttcacga ccgcgccttc gtattgccac gacgcgacgc
    15661 gctcggcgaa ggacggcggc agcacgccgt cgtcgagcag cgtgccgaac gtgacggctg
    15721 gcgttgcgtt ggacagcacc agcggcgcct cgatcatcgt gccgtcggcc agcgtcacgc
    15781 cgcgcacccg gccgccgccg gtcacgatgc gcgacaccgg cgacgagacc cggatctcgg
    15841 caccggcctc gcgcgccgcg gccgcgatcg cctgggtcac cccgcccatg ccgccgcgga
    15901 cgtacgccca ggtgccgtcc atgccgttga gctccccgcc gatcgcgtgg aaggtcatga
    15961 tccaggcggt gccctcctct tcgggaccca gcttcgtgcc gatgatgcct tggccggcca
    16021 tcggcccctg gatctcgggc gcgtcgaaga atgcgcgcac cgtgtcggcg catgagccgg
    16081 cgacggccat gcggtacagc tcctcgccca gcgcggcgcg tacgccgtcc aggcccggcg
    16141 ggtcgggggc ttcgacgagc ggacggatct tggcgacggc ctcggtccag aacgcgcccc
    16201 agcgtccgag cgcgtcggcg tcggccggat tgatgcgcgc gatctcctcg cgcgtgcggt
    16261 cgccgtcgcg ccagacgaag tagtgccgtc cgtcggggag cggcacgaac atctgcgggt
    16321 ccttcggcgt gatcaccaag ccgtgccgcg ccagatccag ctcggcgtgg acgtcgggcc
    16381 gcagcagcga cagcgagtac gaggcggtcg agacgcgaaa gcccggcgcg aggtcttcgg
    16441 tgacggccgc gccgccggtc acgtcgcgcc gctccaggac cagcacgcgc cgcccggcgc
    16501 gcgccaggta cgccgccgcg accaaaccgt tgtgtccggc gccgaccacg attgcatcga
    16561 accgttctac gaccattcga tcgtctccag tgcgccttgg cggatcagcg tcacgaaggc
    16621 cgccgccagc gtggggtcaa gatggtttcc ggccagccgt tcgatctcgg cgagcgactg
    16681 ctcgacggtc caccgcttct tgtaggggcg ttcgtgcgtg agcgcgtcga acacgtcggc
    16741 gattgccacg atgcgcgcgc tcagcggaat gtcttcgccg gcgaacccgg cgggatagcc
    16801 ggcgccgtcc cagcgctcgt gatggaacag cgcgatctct tgcgctgcct tgagcaccac
    16861 cgcgtggctg ccccacagga tccgcccgcc gatgacggtg tgcgcgcgaa tgcgttcgaa
    16921 ctcgtctgct tccaggggcc ccggcttgag caaggtcgag tcgggcacgc cgatctttcc
    16981 gatatcgtgc agcggcgcgg ccagacgaat ggtctcgcat tcgccggcgt ccagcccggc
    17041 ggcggcggcc agcagcgccg acgtctcgcc gacgcggcgg gtgtgttcgg cggtgtcgtc
    17101 gtcgcggtac tcagcggcgc gcaccagccg gtccagcacc tcgcggcggg cgtcctccag
    17161 ctcgcgggtg cgcatccgga ccttttcctc cagcagcgcg ccggtatcgg tcagcagccg
    17221 ccgcagcgcc acgagctcga gcgaggcgcg cacccgcgcg accagcgtcg ctcgatccga
    17281 cgggtcggcg atcgtgtccc gcgcgccggc gtccagcagg cgagccgagt cggcgggcgt
    17341 ggccgcgacc gccagcgcag gggcgtcgcc cagcgcgcgc aacgcatcga ccgcgcggtc
    17401 cggcggctcg tccacgatgg tgagcgccgc gggaacgtgt ccgccgctcc tgcgcgtcgc
    17461 gaccgcggtg aagcctgctt cgcgcaacgt gcctgcgatc gcattcgatg acgacatcac
    17521 gacgatcgtc atcgatgtga cgtcgccggg atgcggggcc gggcggggct gcgcgctcat
    17581 cgccgaagca ctgtacgcga agccgtgcaa gccgggcatc tgttccccgg cggggaagga
    17641 ttgtgccgaa ctgcgtccgc gacctgcgcc gcggcaggct cgttgaccag ggtgaagctt
    17701 acgcggaagg agatggcgca tggctcgcag gctcggactg ctgatcgtgg gattgctcgt
    17761 tctgggggga gcgatgcctg cgaacgcggg cccggacgcg gccgctgcgg agatcgtgcg
    17821 tggaagcgcg cgcgccggct tcgagcgtcc ggccgatgtg ccatggcttt tgatcgcggg
    17881 cctgctggtc gcggcgatcc tgacgctgca ggcggggctg cgctcgcagc cgcgcgcggt
    17941 gcgggtctcg atccgcccac cgaagcaacg ataagacttg gcgggggcca agagaggcgg
    18001 gagcgtcggc ggctcccgcc tctcccattt cttctacgcc tgcgagcgcc gccgtccgat
    18061 cacgtagccg gccgtgccca gcacgccgcc ggcgagcgcg gccgctccgc cggccgctcc
    18121 tgcggacgag cccgcggtcc gcgcggccgc tcgcgtttcc tcggggctga gaacctcgcc
    18181 ggacgacccc aacgtgccga tcgcgcccgg ggagggcagc ggcgatccgg ccacgaccac
    18241 gcccgtcatg ccgggatgca gccagcacgc gaacggatac acgccggcac gggtaaacgt
    18301 gaactcgtag ctcatgccgg ccctcaggtc ggggctttgc aacccgaggc tgggccatcc
    18361 gaaaacgtga tgcgcgatcg catcttcgtt gacgaaccgc accgtcgcgc cgggatcgac
    18421 ccacagcacc gccggcgtga agcagctggc tcgcatcacg accgtggatc cggacccgga
    18481 ctgcggcagg gtccggcagc cgcccccacc ggccatcgcg gcgggcaccg cggccaccca
    18541 caacgccccc gcggccacgg caacgcagat catccgtctc atggctctcc tccgttctct
    18601 tgacgttgat gacggatggc ggcccgaaaa attcccgacg gctacgggta catgcgcgcg
    18661 gcgacggctt cgagctgctc gcgggtcatc ggcgcgctga tcgtgatcgt ggtcccgccg
    18721 ctgcggaacc ggagcgcggg caggtcgtag aggccgatca ggtaccacgc ctcgccgtcg
    18781 ccggcgcgcg ccggacgcgt cacgaccgta tcgtcttccg tctcggcgcg cgggccggtc
    18841 tgcaccagga tcgtgaacgc gccgcgcgca tagagcaccg catcaccagc cggcccggat
    18901 cccgcgtgca cgacggagaa tccgtcgggg gtggccgccg gcggcatcga cagcgacgcg
    18961 ggcggctggg ggcgaaagcc tccggatccg gccggaaccg tgggaagcgc gaacagcgaa
    19021 tcgtcgagag tgacgttgta ggcgatcgcg gtgacacgct tggcgacggt gcgtcgcgcg
    19081 cccgcggcct gcttgcgaag aacgaccgcg acctcgctcg acagcaggct cgcctgcgac
    19141 gcgtcgatca gcgcttcgcg caccacggtg ccgtcgggcg attccagcac gacccgctcg
    19201 gtggcgcgcc ccgcgacctg cgggccgggt ccggtgacgc ggcccgcgag cgcgagggag
    19261 ggcgacagcg gttccggcac catgttcgga tccggcggaa ggccggtctg cagcgtggcc
    19321 gcgctgcctt ggatgagcgc gcgtgcgccg ggaaggcgga tctcggtctg cgtcgcggtg
    19381 ccccgtgcat cggtgacggt ccggtcgatg cgcgtcgtag cgggcgcccg gtagcggatg
    19441 cgttcgatgg tcgtggtgcg cgtgccgtcg aaggaggtct cgacgatctc ccgggtgacg
    19501 tcgagcgtgc ggatggtcag cagcgcctcg gccgcgccgg ccggctcgac gcggaacccg
    19561 cccgccggca accccgacac cagcagcgcg atggccgcga ccactgccgc ggcggcccag
    19621 cctgcgagcc tcggccgccg gatccgccgc gttcgccgcg tgctcatcga cggcacggcg
    19681 aaaccatccc gcagcgccgg ctcgggcagc gcccgcgcga gcgcacccag ccgcgggatc
    19741 gcgtgcgtga acgcacggca ttcgtcgcaa cccgccagat gcgcctccag ctcggcgatg
    19801 ccggccgcct cgagctcgcc gtcggcgcgg gcgctcgcca gcgtgcgcgc gtcttcgtgc
    19861 cgcatgctca tccttcgacc gctcccatca gccgctcccg cgcacgggcc aggccggaca
    19921 tgaccgagcc aaccgggcag ccgagcacct cggcgatctc cttgtaggag agtccgtcgc
    19981 gctcgcgcag caggaacatc tcgcggtggt tctcgggcag cgccgccaac gcgatgcgca
    20041 gccgctggat cgactcggcg tgctcgtggc ggacggccgg atcggagccc tcggccgcaa
    20101 ggtccccggc cgcatcggcg ggcgactccg cgacacggcg ggcgcgcatc cgcgcttggt
    20161 cgcgcaccac gtttccggcg atcgcgagca gccagccgcg catgcgctcg tcgccgccgc
    20221 gataggtctc ccggccgcgc caggcgcgca gcaaggtctc ctgggtgagg tcttcggcga
    20281 gcgcgcggtc gcggacgcgc cgcaacacga atccgaagac ggctccggca tgccggtgca
    20341 cgagcgcggc gaacgccgcc tcgtcccgcc cgatgccgtc gccggccgtc cgttccacgt
    20401 gctcactcaa gcatggacgg atgcggcggc cgggtattcc cggcccgggg cctgcgacgc
    20461 gcggtgcgcg caggctgcga ccgcgtcagg cgcgcgtcca ctcggcggga aggatgcctt
    20521 cgaagccttt catcatccag gtgacggcga ccgcaaccat cttgtcgcgt tccatcggct
    20581 tggcagccag ccaccagcgg gcgagctcct cggcgacggc gacgatcgcg cggttgtaga
    20641 gctccaccac gaccggcggg gcgttgacgg gctcggcgag cgcgtcggcc cgatcggtca
    20701 tgatcgtgat gatctgctcg cggacgcgca cgccccgctc cacgatgccc gggtccgacg
    20761 tcccgggcgg tccgaacatc acccggaagg cgctcgggtt gacgtcggca tagtccagga
    20821 aggccgaaag gccgctggtc agcgcgtcgc gcagcttcat tcgcccggtc gtggcgagca
    20881 cacccatcat gtgcgcgcgg aatcgttcct cttcccgttc cagcagggcg aagtagatct
    20941 cctgcttgcc gccgctgaag cagtcgtaca ggaccggctt ggtggtgccg gccagacggg
    21001 cgagcgcctc catcgaggcg tccgcgaaac cgccctgggc gaacagctcg agggccgcgt
    21061 ccaggaccaa gggacggcgg cgagccggcc cgagatactc ggcgcgcttc ggtttgacgg
    21121 tcttcggcgg ggtcatcacc ccattgtgga cccccgggat gggcgcaaag caaggtttcg
    21181 ggcgaattta accatcacgg acaagcctca ggttcttgac gatctgtcac gaaacgatca
    21241 gaatgctacg gcgcggtagg tttcgtgtcc ggaaagcaag cggccacgga cacgcgtcgt
    21301 tccccggaac gcggcgatcc cgcgcggggg aggacttccg gcgagaggcc acgaactaca
    21361 cgccacgctt taccgcagca aacgttgaag aaaccgaacc gggagccggc catgattcgt
    21421 tggaagcgtt catgggagga cctctccgag gcccttccgc tcgacgtgat gccttgctcg
    21481 aacggcgagt tctatccgcc gccgcccacc aaggaacaaa aagccatcat ggagctcgcc
    21541 gaccgtgagg tcgagcgcgt ccgcaagaag ttcaacatga gccgccgcga cttcgttcga
    21601 acgaccgcgg ccatggcgat ctgcttctgg gcgaaggaca cgatctacgg cagcgcgcgc
    21661 gcacacaaca cggccacgac cgaggcctgc gatctgcagt gggacgaggc cgtcggcgac
    21721 atgacgctca acaacctgcc gggcgagttc atcttcgacg tccagtcgca ccacgtcgag
    21781 tccgacggca tgtggcgcgt caccaacccc ggcttccacg cattctttgc cggtgtgtgg
    21841 tcgcaggccg gtctgctcgg gtccgccccc ggttaccggc cgggcggcaa cttggacccg
    21901 gggtcgggaa acttctcggt ccgcggtgcg ggtgcgggcg agctggaccc gatcgagaac
    21961 ctgtcgcgct atcactatct gaaagagctg ttcctcgact cggcgacgac gatgaccgtg
    22021 ctgtcggcgg tgccggccgc gcccgacatc tcgcagccgt tgccgatcga gcgcgcggcc
    22081 ctgaccgtgc agatggtcaa cgagctcgcg gggtcggagc gtacggtcat gcacgccttc
    22141 gtcatgccga accgcggctc ggccgggacg gtcgcgtcgt cgctgggcaa agagcccgtg
    22201 tatatgcagc gcgagttcga gatcatggaa gagcgcgccg tgaagtgggg cagcatcatc
    22261 cgcggctgga agacctatcc cgcgtggggc gacattccct acgccagcgg ctggttcttc
    22321 gacgatgagc tggtcggcca gcgcttcatc gaccacgttc gctacatcag tcagaagtat
    22381 cccaacgtgc ggccgaacat cgccacgcac aagggcttcg cgcttccggc cttcgaccag
    22441 cgcgcggcct cgccgcgcga catcggtccg gccgcatcgc gcaacccgga cgtgaacttc
    22501 atcgtctacc actcgggctt cgacggcgag acgatgacct cgtacggggg ccaggccacc
    22561 gacccgacca acatcgccgg cgacggaccc aagggcgacg cgcgcgtcag ctcggccgac
    22621 cgcaccgtgg actcgctcat caagagcttg cgcgagaaca actgggacgc aacccgcttc
    22681 ggcgcgatcc ccggccagaa gtgggcgaac attccgaacg tgttcgccga gatcggctcg
    22741 acctggcgca ccgtcaccgg ccgcgggcct gtgcaggaag cgcatctgct cggcaagctg
    22801 atcacctacg tcggtcccaa gcggatcgcg tggggaaccg acagtctgtg gttcggctcg
    22861 ccgcacgccg agatcatcgc gatgcgccgc gtctcctaca gcaaggcggc caaggtctgg
    22921 gacttctacg gcctgccgta tgcgatcgac ggcgacgcgg acgacccgac cgtggacacg
    22981 cgcaacccga tcagctactc gccttcgcat ccgaacgcgg gcgtgatccc gggatggccg
    23041 acggatggga cgtcacaccc cgagcgttcg atccgcaact ggatcttcgg tcgcgcggcc
    23101 gctgaggtct acggcgttga tgccgacaag cacctgaacg accgggcgaa ccatccgatc
    23161 agttgcgaca acgtgcagaa gatccgcgac gcgtacatcc tcaaccagta cacgccgaag
    23221 gagcaggtgc cgatggcgtc caacgcgctc atcggcgcgc gcactccgca agagctgaag
    23281 atcgaaagga tgaatcagcc gtgggcgccg taatccgacg cacgaccgtc ctcatcgcac
    23341 tgctcgcgct gctcgtgccg acggcggccc gggcgtcgac caccacgtat ggccctccct
    23401 acgggaacgg acccagcggc ggtgacacct acaaccggat cgatcgcaac ggttcgaccg
    23461 gcgagatgtc gatccttcgc tacaacccgg ttccggccaa cgggacgttg ggctgcgccg
    23521 gcaacggtgg atgggcgacg ttcacccttc cccacacggc cgcctcggac atcgtttcgg
    23581 tgacgttgtc ctttagaggc gcgatcgtgg atccgtactc gttcatgtcg ctgtcggtct
    23641 accgggacga ccatttcgtg gacacgcaca tcgagcgagg tccgatcctg ggtgacggca
    23701 cgatcgtact cgagccccgg accgccgcca gcggagaagt cacgctgatc ttcggcctgc
    23761 aggccggatc gtcgtgcccg aacgtccagc tcgcccgtgc ggtcttcgac tcggtgatcg
    23821 tggtcgaagc gtagccctgc ggcaattttc ttcaggcacc ttggtcgtcg tcggtgacga
    23881 ccaaggtgcc tttcatttcc gggtgattgc tgcagaagaa ggcgtaggcg cgcgggagca
    23941 gatcggggac gccttcgacc tcggcagcgc tgccgaggtt ttgctggtcg gtccagaaca
    24001 gcggccagcc gtcctcgtcg tattcgaccg agtagatcga gtgcacgccc agattgtcgg
    24061 cgttgacgaa gagcaagcgt cgtccctgct cgacgatcag cggcccgagc ggcagcgcgt
    24121 cgtcgcccgg tacgaagcga agcgcggccg cggtgatcag ggccggctcg gccggggacg
    24181 catgagcgcc cgtgagcccg ctcaaagcaa gcagcattgc aagagttcgt cgcacggctt
    24241 gttgattcga cgaagccccg gcgcagccct gccgatccgc cggctgcggt cttggagcct
    24301 cattctgcct tcccatggaa ccgatgggcc tttggcctag tctggtggtc gtggaggacg
    24361 tggcgacggg cgagccggtc gaggcgggcc gcatcgaggg ccgtcgcttg gagctgctgt
    24421 accgcgctca tgcaaccaag gccgcccgaa ccgcatacct gctcaccggc gacgccgcgc
    24481 tcgccgacga cctggtccaa gaggcgttcg tgcggctcgc aggccggttc gtggactggc
    24541 gccgcgagga cggcttcgag ccctaccttc gccgcacaat cgtcaatctg ttccgcacgc
    24601 gactgcgccg catcgagcgc gaacgcgagc ggcatcggcg cgtggcggcg agcccggtcg
    24661 ccgcgggcgg cgatctcgcc gacctgatcg cggtgcgtga cgcgttgatg cgtctgagcg
    24721 agcggcaacg cgccgcggtc gtgctgcgct actacgagga tctgcccgaa gccgagatcg
    24781 ccgacctgat cggctgccgg cccggaaccg tcaagagcct gctgtcacgc gcgatggacg
    24841 cgctgcgcgc cgagctatcg aggtgagcgc catggatgtc aagcagttgt tgagcgatct
    24901 ggccgagcgg cagatgccgt ccgctccgcc gctgcgtccc ggcaccgtgc agcgtgcgca
    24961 tcgccgccgc gctatggtcg cggccgcgac ctcgctcgcg gtcgtcgcgg gcgcgctggg
    25021 cgtgtgggcg gggatcggct cgctttcgcg gccgcggccg gttccgccgg ccgagcgcga
    25081 cgcctcgttc tcgatgatcg cgctcgacgg aaccgaggcg accgcgctgg ccgcgcacgg
    25141 cgacgctgca tgggccgcga ccgacggcaa gctgtatcgc gtcgccggct ccgagcccga
    25201 gcgcatcggg ccgttccccg acacctactt gggccagcct ttcgagcagc ggatgcagat
    25261 cgacgacctc gccgcgaacg aaaacggcgt ctgggttgcg gcatcgccgg tcggtgcgcg
    25321 atgcggcgag tcggatgcgg tcgccaaaga cccgtgcggc gatgcgcgat ggatcccggc
    25381 aggcggtgcg ccgcagcctg cccgcttctt cgccggttcg cgcggcggga tcgcgatcgg
    25441 gaacggatcc acctgggtcg gcggcacgac cgtgatgtac aaggtgacca cggaccagag
    25501 ggacgcgccg gtcgttgcgc cgcgaaaagg cgtccgctcg atcgtcggga tcgcttcgac
    25561 gctcgggcag gtattcgctc tcgttgagcc ggacgacgga cgcgtcgatc gcgcgctgct
    25621 gtcgatcgag cccaacgaag gcggcggact cggccgtctc gacctgccgg gcgcgcttat
    25681 cgtgcgcgcg ggcctcgggt cgctgtgggt ggcggcctgg cacgagggag ccgccatgct
    25741 ctaccagatc caccccgagc gcttctcgat cctgcgcacg atccggctga tcgagtcgcc
    25801 cggcgatgcc gccaaggtct tgatcaaagg tctcgcgttc tccggctcga ccgtctacgc
    25861 ggccgccgca cgcggcgacc aaggcatcgt gctcgccttc gacgcgcgca ccggcacggt
    25921 cgagacgctg cgcgccgcgg gtgtcccggt agatattgca gccggacacg aggcgctctg
    25981 ggtgctgcat tcgcggcccg cacgtctggc gcgcatgacg attcccgatc catgggacgc
    26041 gcacgcgccg atcaacgatg acgaacgagc tgaggcggtg ccggaacgcc ccggcctgga
    26101 gcacccacgc atcacgcgca tcgaccggct cgaaccttcg ggcgaccggc ttacgatcgg
    26161 gttcctcggt ggcgactatc cgtgcggaat gctcgatcac gtcgagttat gggaagatcg
    26221 cggcaggctc atggtcctgg tcgtcgaggg cgtgcgacca acggtgcgtg acgggcagtg
    26281 gggatgcaaa agcgtcggca tcgggcgaac gaccaccgtc gagattcccg agcggctccg
    26341 cggcctcgaa ccggccgacg ccgcacagga ctaaccgcgc gccgtcgcct tcgccgtgat
    26401 cgttgttgtc gcacccctgt tctagattgt tcgcatgctt ccgatacgtg aagtcgatgc
    26461 gagcatcgcc gagctgcgtg acgtcgtcgg tcgcgtgcag cgcgcgggtg cggtgcgcga
    26521 cgtttccttc atcgtcgagc tgcttcgcgc ggccgaccgg ctgaccgcga cggcgctcac
    26581 ggcgatcgcg cgcgacaagc tcgacgaagg cttcggtctg ccgcccgcga tcgtgctgca
    26641 gatggacgcg cgacgcacct cgtgggaggc acgacacctg ttgcgcgcgg cgaccacgct
    26701 cgccgccatg ccggccgcta ccagcgcggc cgagatgggt ctgctttcga tatcgcagct
    26761 tcgggccatc gtcgccgcgg cacgcacgtg cgacgccgcc ggacgggcgc gcatcgacga
    26821 atcgatcgcc gacgcggtcg tcgtacacgc ggacgcggac cccgacgcga tcgtcgccgc
    26881 cgtggacgat caggccgcga ccctgcgccg cgaccggacc gaacgccgcg agcaccgggc
    26941 cgtcgaagcc ggcttcgtgg cgatccagcc aacgctcgac ggccgcgcct cgctctacgc
    27001 cgaaggcgac gccgagtcga tggcgaccat cgccgacgcg ctcgaccggc aggcaggtcc
    27061 gcctcgcgac gaccgaacgc gcgcgcagca gtgtttcgac gcgctcgtcg ggctgtgcgc
    27121 tgccggcccc gccggcgatc gacgccggcc gcgcgtgctc gccacggtcg atctcgccgc
    27181 gctcacgcga tccgaacgcg acgcggccgc acgattgctg tggtcgttgt ccggcggatc
    27241 gccgcggctg tcgccgatcg cgacgtgcac gttgctgtgc gatgcggatg tgctgccgat
    27301 catcttcgag cgcggccagc cggttgcgat cggcgacgcg accagcccca tccccgcgaa
    27361 gatccgccgc gccgtcgcgg cgcgtgacca aggctgccgg ttccccggct gcggatcgcc
    27421 gctcgcatgg gcggacgcgc atcacatcac gccccgacac cgcggcggcc ggtccgatcc
    27481 cgacaacctc gtgatgctgt gccgccgatg ccatcgccgc gtccacggcc acggatggat
    27541 cgtgagcctc cgagccggcg gcgacatcga gttccgtcac cgcggccgcc ggtaccgcag
    27601 cccgccaccg gccggatagc gatccgtttc tcccacagtt gtatcgggta gtttcgtggg
    27661 aatggcggcg aaaaccccgc cgtatgagac tccgcttcgc gaatccgacc tggatcgcgc
    27721 gcgtctgcac gcgccctttc cgcgctccgc cttcgtcgcc atcggcgcat gggtcgcgtg
    27781 gttcgcgctc atcctcagcc cgttgaccgg tgtgcattgc ggtggaggtg acgcgacgca
    27841 gtgcacgccg ctcgtcaacg accttctcct tcccgcaacg tccgtgttcg ggatcgcgtc
    27901 gctggccggc ctggcgttgc ggcgttcgtg ggcggggatc acgtcctccg ccggcgccgc
    27961 aaccggactg tggatgggca tcctcgtcat cggcgatgct ccccctcttg tcgcgggcga
    28021 ggcggccatc ttcgccctgg ccgtggtggg aagcctcatg acggcggcac acaatcgcgg
    28081 catcctcacg cttgcgggcg acgagccggc ggacccgcca ccggcagaaa gcctgcgtcc
    28141 gctcggcgcg ctcgttcggc gccgcatcgc gctggcggtc gcgttgctgc tcgccggtgg
    28201 gatcgcaatc gcgatcgtca agtcgaacga gcgcaacgca cgccgcttcg aggcgaccgc
    28261 accgcagacg accgcgacgg tagtcgacgg cggcgatccg ctccgggtcc gctaccgtca
    28321 tccggtttcc gggcggatcg tcgaagccga gatcccgaac ctcctcgagg tctacgaacg
    28381 gggtgagcgc gtgcggattc gttacgacgc cggcgatccc gggcgcgtcg gcgtcgtcgc
    28441 cgagccctac gacgcggagt cgccgctcat cctgttcggg ttcctgccgc tcgccggcac
    28501 gggtgcgctc gccgcatcgg cgtggtgggt tcggcggctc cacatgattg ccgggcgtcc
    28561 gggccggcgc gtgcgcacgc ggatcgacgc caccgagata gacgccggcg aacaggatcc
    28621 gccatcgggc atcgtcgtcg taacggtgtt ccctgtggac ggcactgagc cggtcgccac
    28681 gttcgaaacg cttcccggcg gcattccgtc ggatctgccc tacgacggtc ccgccgaggc
    28741 gcgcggcatc atcggatggc acgcgccgct cgtcgttcgc gtcggcgatc tctggctgtg
    28801 gcctcgccgg cgcgtccggg tcggtcgcgc gctggcgcgc cttttgtcca aggtcgagga
    28861 aaccctgcag catgaacggg acgcaaaagc cgcagccgag gcggcgaacg agatccgtcc
    28921 ggtcgaggcg gcacccgatc ccggatggca cccggtgcgc ctgttccggc ggcgctcgca
    28981 cctcacactg ctcgaatcga tgcgcaagca gacgctcgtg tccgtcatcg tcaacgccgg
    29041 ccttgcgcaa tggggtgggg tgatgatgct taccggccgc ccgtcccgcg tcgccggaat
    29101 cgtcgccgtc gtcgccggac tcgcgtgggc cgatcccgcc gacgcgacgc cggccgaact
    29161 gtaccgcttg atcgactttc ggagcacgat cgccggcttc gtctcgtcgg tccctgccgc
    29221 ggccgtcgcg ctgacgttct tcttcttcgg agcggggccc gaagccctcg ccatcgccgg
    29281 accggccggc ctgctgtgcg atgccctggt ggctccgacc cggtcatggc tcgcccgcat
    29341 ccaagaggcg ctggacgaac aaggttttcc gctgtcggtc gccgcggtcg tctcggcgcc
    29401 cgcagtcgaa atacgaaggt ggcgtcagcc aagggagtag ctgcatatca cctgtgctcg
    29461 acgggcgcgc agcatcgggc cggccgacga tggggtcgtc gccgatcgtc aggcgcatct
    29521 atcgagcgag ctcgcgcacc acggccggca ggattccgcc ttcccggaag taccggacct
    29581 cggttggcgt gtccagccgg cagcgcgtgt cgaactcgac cgtggtgccg tcgtcgcggc
    29641 gtgccacgac ccgcagcgtc tggcccggct tcaaatccga cagaccggtg atgtcgaacg
    29701 tctcgcggcc gtccagtccc agcgacgacg cggtctcgcc cggtgcgaac tccagcggca
    29761 gcacgcccat ctgcgcgagg ttcgagcgat ggatgcgctc gaacgactcg gcgatcacgg
    29821 cgcgcgcgcc cagcagcgtc gtgccctttg cggcccaatc gcgagacgac ccggacccgt
    29881 actctttgcc ggccaggatc accagcggca cgcccgccgc gcgatacttc acgctcgcgt
    29941 cgaagatcgt catcgtctcg ccgcccggca ggtgcgcggt cacgccgccc tcgctgcccg
    30001 gcaccagcag gttacgcagc cggatgttgg cgaacgtgcc gcgcatcatc acctcgtgat
    30061 gaccgcgccg agcgccgtag gagtgcagct tgccgggcgc caccccccgc tcgcccagcc
    30121 agcgcgccgc cggcgagtcc ttcgcgatcg agccggcggg cgagatgtga tcggtcgtga
    30181 ccgaatcccc gacgaacacc aggcagcgcg cccccgcgat gtcggtgacc ggcggcggct
    30241 ccatcccgat cgaggacacg aacggcggct cggtgatata ggtcgaggac ggatcccact
    30301 cgtacagatc gccttcgggc accggcatcg acttccaccg atcgtcgccg tcgaacagcc
    30361 cgccgatgcg gctgcgatac gactcgggat tcatcgccga cgcgaccgtg gacgcgatct
    30421 cttgatcgct gggccagatg tcgcgcagga acaccggctt gccgtcggtg ccggtgccga
    30481 gcggatcgtt gttcagatcg atgttgaccg tgccggccag cgcatacgcg acgaccagcg
    30541 gcggcgaggc caagaacgag aagcgcacct gcggatggat gcggccctcg aagttgcgat
    30601 tgcccgacag caccgccgac accgccaacg cgttctcgtc gatcgcgtcg gcgatcggat
    30661 ccagcaacgg cccggagttg ccgatacagg tggtgcagcc gaagccgacc gcgtagaagc
    30721 cgatcttctc gagatcgtcg agcaagcccg cgcgcgtcag atagtcgagc acgacgggcg
    30781 agccgggcgc gagcgaggtc ttgacgcccg ggtccacgcg caggccgcga gccaccgcgt
    30841 tgcgggccag cagcccggcg ccgatcatta ccgacgggtt cgaggtgttc gtgcatgagg
    30901 tgatcgctgc gatcgcgacg gttccgtcgg cgacctcggt gtcgccgcgc tcggtcgtca
    30961 ccgggatctt cttgatcgcc ggctcgcccg actcgccgcg cagcgtcgca agctcgcggc
    31021 ggaacgtcga tccgacctcg cccacgacca cgcgatcttg cggccgccgc ggcccggcga
    31081 gcgacggctc gatcgtggac agatccagct cgagcagctc gccgaagagc ggcgcggcgc
    31141 cgtggtcgtg ccacagatgc tgctctttgc agtagcgctc gaccagatcg atcaggtcgt
    31201 cgctgcgccc ggtggcccgc atgtaggcga gcgtctggtc gtccaccggg aagaagcctt
    31261 cggtcgcgcc gtactcgggc gacatgttcg cgatggtcgc gcggtcggcg acggtcagcg
    31321 ccgagaggcc cgggccgtag aactcgacga acttgccgac cacgccgtgc gcgcgcagca
    31381 tctcggtcag cgtgagcact agatcggtcg cggtcgcgcc cgccggcatc gagccgtcca
    31441 gccggacgcc cacgaccgac ggggtgagca tcgtcaccgg ctcgcccagc atcgcggctt
    31501 cggcttcgat gccgcccaca ccccagccca gcacgcccag cccgccgatc atcgtggtgt
    31561 ggctgtcggt gccgaccagg gtgtcgggat acgcgacccc gttctcgtcg gtcatcacga
    31621 catcggccag gaattccaag ttcacctgat gcacgatgcc gctgcccggc ggcacgacgc
    31681 gcatcttgcg gaacgatttc tgcgcccagc gcaggaacgc gtagcgttcg ccgttgcgct
    31741 cgtattccag gtcgacgtta cgcgcgaacg cgtcgcgcga gccgaagaag tcgacgatca
    31801 ccgaatggtc gatcaccaga tcgaccggca cacgcgggtt gacgcgcgag gtgtcgccgc
    31861 ccaaccgctt gaccgcgccg cggatcgcgg ccagatcggc cacgaccggc acgccggtga
    31921 agtcctgcag cacgacccgg cccggcagga acgggaactc gaccggcgcg tcgcccggcc
    31981 agccggccag cgcgtccaga tgcaggcgct cggcgtcggt tccctgatga cgcagcacgt
    32041 tctccaggaa gatgcgcacc acgaacggca ggcgcgcgat cgagccggcg tccagcaccc
    32101 tgtccagccg gtagagcgcg acgctgccgc cggcggcctg cagccggtcc agcgcgccga
    32161 gcgggtcgtt gaggctcatt cgggctcctt acgcgtcacg aagtcgaggt gatttccctc
    32221 atcctacgtc ggcggagcgg cgcgtgtttc cggcggattc agggccgatg agcggcccgc
    32281 atcccgagca ccagcaccgc gaagaccagc ccgagggccg ccacgccgcc cgcgatcggc
    32341 gccaccgcgg cgagcgctcc cgtctccggc gattcgaaag gacgcacccg cggtccggcc
    32401 gctgcgcccg ccgggttcga gccgggcgac gaagccgccg gcgtcttttg cgtgccgtcc
    32461 ggcgccggag ccggtccgcg ccgctcgccg gtccgcgaac ccgggtccgt cccgtccggt
    32521 cgcggccgcg ggccggccgg gacggacccg ggttcgcgga ccggc
//
LOCUS       DATLFO010000375        11755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_69116, whole genome shotgun sequence.
ACCESSION   DATLFO010000375 DATLFO010000000
VERSION     DATLFO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69116"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VM841_15415"
     CDS             <1..412
                     /locus_tag="VM841_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91610.1"
                     /translation="DTIVAGDTLTGRTRPIGATLSKTRIEPGAPAPDALSAATDHVAR
                     VTTGNFLGETRRKASNLTDAVASLAGHPRVEDVRVRGLIAGIEVDTAERAASAAEAIP
                     YASAAGNVVIVSPPVDVSDAAIDGLTAAIIAALR"
     gene            complement(511..1041)
                     /locus_tag="VM841_15420"
     CDS             complement(511..1041)
                     /locus_tag="VM841_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91611.1"
                     /translation="MPRIAVVGSFSTGKTTLAEVAAQELKLPLLPEVAREVAAMGFKL
                     DKDATVETEALIFLKQYRNEMTHRDFIGDRSLIDVMTYAGWVLDHQERRKEFALWDEC
                     VEIARHHLRSQYSHVFYLPIEFDIVPDGLRPMDPDFQRDIDERMVRIMHDNDIDYEPL
                     RGTVEERLEALSKHLG"
     gene            complement(1055..1555)
                     /locus_tag="VM841_15425"
     CDS             complement(1055..1555)
                     /locus_tag="VM841_15425"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVL91612.1"
                     /translation="MTMIVEEIATPIGTLTLAQAADALVHVAFDGAWNETPLAAIYGP
                     ASSPPSESEALKRVRAYFDGDLRAIDAIPVSPRGTAFRLAVWDAMRAIPPGEPVSYAE
                     LAARAGRPSATRAAGTACGANPIPVVVPCHRILRTGGGIGGYGGGLAAKRWLLAHEAA
                     NANVRG"
     gene            complement(1552..2997)
                     /locus_tag="VM841_15430"
     CDS             complement(1552..2997)
                     /locus_tag="VM841_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HVL91613.1"
                     /translation="MDLDGDRCYRALLSRDRRFDGRFFTGVLTTGVYCRPVCPAPTPK
                     QRNVRFYPSAAAAEAAGFRPCLRCKPEHSAGIPAWPAVPEPVSRALSLIAGGALDDGG
                     VDGLARLAGVGARQLRRLFESHVGASPLAVGRARRVHFAKRMLDETDRPVGEIAFAAG
                     YGSIRSFNHDFALTFKRPPSQVRRSGRRGPAGAITMRLAYRPPLDWESLIGFLATRAT
                     RGVEAATADAYRRTILADGAPGSIEIRHAPGEPYLLLTVTTASPARLLSVVERARRIA
                     DLGADPMRIDEVLAADDALRHLVNARPGLRVPGAWDGFEVAVRAIVGQQVSVAGATTV
                     ASRLVDLFGKPVETDLGEGLTHLFPSPATLANAEVEAAGLRGAQARAIRALARAVDDG
                     RVRIDSSDGLIGTLTALPGIGEWTAHYVAMRAGGDPDAFPAADLGLRKAFGQGSAVSA
                     RALAAAAERWRPWRSYAAMHLWRSLSDKEPR"
     gene            3157..4149
                     /locus_tag="VM841_15435"
     CDS             3157..4149
                     /locus_tag="VM841_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HVL91614.1"
                     /translation="MRIKASKLVVAGLIAAAIVTANPAGAVEPCPAVACVVVNGTVVA
                     PHGERVADYTVIAQRADGYHKTSKSNATGAFTFTLPLPTPNQCYQIAGMSDPFYTVSN
                     VPQKQCSSSTVTLHPRYRINGLSGQQKVYLGSAAENVEIPVEVSALSRTYPAPFANDP
                     LPYVFSHHHPDDKVSGLDDHVHHGATGFFDPPVVRKIADGVWQYRWIDSIKLPGGQPG
                     YYDMDWGRGTSVFNPMMECRMIWFGYGISKSSPAKATPGTIVTIDGQRLGTEAGAVVI
                     KGSGQVTTISGSSILEWTPTRVKFIVPPTAKSGWVEVVPPSGVPTNAQPLGIGV"
     gene            4659..4934
                     /locus_tag="VM841_15440"
     CDS             4659..4934
                     /locus_tag="VM841_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91615.1"
                     /translation="MHLVLGPAALLVIAVITVGVVQEGGAPLGWDVAGLVFTAAVLFG
                     IPLAIMRSAWVQARGKRPIEQHPVARSESPWRAAHPRAEEAPPRKAA"
     gene            complement(4942..5184)
                     /locus_tag="VM841_15445"
     CDS             complement(4942..5184)
                     /locus_tag="VM841_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HVL91616.1"
                     /translation="MGKQRLHLTFPERLIQEPVIYNLGRKFEIVTNIRRANVEDEVGW
                     VILEVAGDADAIAAGVAYLEEMGVQVGRIDGDLVEG"
     gene            complement(5194..6012)
                     /gene="larE"
                     /locus_tag="VM841_15450"
     CDS             complement(5194..6012)
                     /gene="larE"
                     /locus_tag="VM841_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HVL91617.1"
                     /translation="MSIDRLREIISEIGSCAVAYSGGADSAFLADVAHEIHGADRCVA
                     VTAVSESLASSEREAAAALARQRGWRHIEIETREIERAEYRANEPDRCYHCKTELFER
                     LDELGPSLGVEAVLVGTNLDDTGDFRPGQRAARERGVRAPLLEAGLGKDEIRALSRER
                     GLPTWDKPASACLASRIAYGVEVTAERLDRVGRAEALLRSMGVRELRVRDHGELARIE
                     VPVDEIPRLASSPARETLAEGLAELGFAYVTLDLQGFSSGSMNRTLLRIQPARG"
     gene            complement(6022..7050)
                     /locus_tag="VM841_15455"
     CDS             complement(6022..7050)
                     /locus_tag="VM841_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVL91618.1"
                     /translation="MTPSPNELRARIDEALTGFLSAVRVDVAAADPAALPMIDELSRV
                     VGAGGKRLRPLFCYWGHRAAGGEDSDAIVRAGTALELLHTFAIVHDDIMDGSPLRRGL
                     AASHVAMGSGRFGTSAAILTGDLAFALADRLLLEAGFDAVRLSAAFDWFTRMRLEVIA
                     GQFADLVAAERREADEPAARRIAALKSGAYTVEKPLVIGAALAGATPQVIEALTAFGA
                     PLGEAFQIRDDVLGAFGEPSVTGKDAEGDLREGKRTVLVAKARAQASAEDRTFLDAAL
                     GSPDLTDADIERVRDVLRSSGALDATLALIDELHAKALAELEAAPLISDVKQALRVLA
                     ETAVSRDA"
     gene            complement(7100..8245)
                     /locus_tag="VM841_15460"
     CDS             complement(7100..8245)
                     /locus_tag="VM841_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HVL91619.1"
                     /translation="MATPQPAPPPDGSRATPDQIRRWTAYYEDELAGAALYRALAAAA
                     DSEARREVFSKLAGFEEKHAAHWESLLRLAGVEPLPPRVGMRVKVLGWVARTFGVESV
                     LPVVLRAESADAAKYDAAADAAEGMAAEERGHGQTVAAIAQDRADRIAAREGRHRVGA
                     GGALRAGVFGVNDGLVSNLSLVVGVAGGTGNAKIVLLAGVAGLLAGAFSMAAGEWISV
                     RSQTELYEREVEVERGELEAFPEEEEEELALIFRAKGLSPDTAATVAREIMARPEVAL
                     ETLTREELGFSPDDVASPWVASISSFLAFSLGAFVPVVPYLFATGAAAVWSSVIASGL
                     SLFFVGSALAIFTGRRPMHSGLRMLAIGSAAAATTFGIGSAIGVSIT"
     gene            8308..10137
                     /locus_tag="VM841_15465"
     CDS             8308..10137
                     /locus_tag="VM841_15465"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVL91620.1"
                     /translation="MTRFRKLALATTATTYLLIAAGGLVRATGSGLGCPTWPGCYPGR
                     FFPPLEKAAMIEWTHRTIVVFLTIMIAAMTVWALRRFRAERPVMVPSVAAFGLVLFQA
                     ALGAITVERELDPVVVTAHFATAMVLVGTLISATIATFRLREVTGPRVQADFAQLALV
                     AAAGTFILLLVGAYVRGLGAGLAFPDWPLMNGRLLPQAGRIETPHFVHRLLALFAGAL
                     VVALAWRSNRTASRDAHPRPVRLLALAAAGLYLVQAFVGALNVWTDLSEPTIVAHVAL
                     AALSWGAVLGLAVVSRGLSRQNEQDVDATSPERAASGRGLGRAKAVVATYFRLTKPRI
                     IVLLLITTVPTMMVAFRGWPGLGLVLATLLGGSLAAGGANAINCYVDRDIDAKMGRTS
                     RRPLPAGEVTPARALEFGIALGIVSFAYLWTFVNLLSAALALSALLFYVFVYTIGLKR
                     STPQNIVIGGAAGAVPVLVGWAAVTGTLTWAPVVMFLIVFFWTPPHFWALALRFSGDY
                     AKAGVPMLPVVAGRRETLKQILLYTVVLVAVTLALTPIGDMGMVYLAAAAALGAWFIR
                     GAAILWRRDTNQAAWSLFKLSINYLALLFVAMAVDSVVHVAVA"
     gene            10313..11350
                     /gene="coxB"
                     /locus_tag="VM841_15470"
     CDS             10313..11350
                     /gene="coxB"
                     /locus_tag="VM841_15470"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVL91621.1"
                     /translation="MTTKRARRIVKVLAGLAALGLLAACSNVGPQDYLHPEGPVAEQA
                     DSLFKPVFWIATAIFFVVEGLMVYAIFKFRERRGGSDPVQVHGNTKLEIGWTIAPAVL
                     LAALAVPTVAMIFDQAAVPAEGEDVVRVKVIGHQWWWEYEYTDVDPPVKTANELVIPV
                     GKKVFLEVTSADVIHSFWVPKLGGKQDAIPGRMNSMTLIADKPDVYHGQCAEFCALSH
                     ANMRLRARALPQAEYDAWLAEQQKDAVVPTSGAAAEGARAFKEFQNGTCMACHTIRGM
                     EGASGTVGPDLTHFADRLTFAGSIFDMNEENLAKWLRDPPGVKPGSIMPNYHIPEETI
                     AAMVAFLLSLK"
     gene            11360..>11755
                     /locus_tag="VM841_15475"
     CDS             11360..>11755
                     /locus_tag="VM841_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVL91622.1"
                     /translation="MSSVAVSSRSKGLFARPSHPKGIWDWLTTVDHKKIGILYGVTAF
                     IFFLIGGLEALLIRLQLASPDGTVLSADAYNQIFTMHGLTMIFLVVMPMSAAFINYLV
                     PLQIGARDVAFPRMNAFSYWVFLFGGLFLY"
BASE COUNT         1756 a         4226 c         3962 g         1811 t
ORIGIN      
        1 cgacacgatc gtggccggcg acacgctgac cggccgaacc cgtccgatcg gagcgacgct
       61 ttcgaaaacc cgcatcgagc cgggcgcgcc ggccccggac gcgctctcgg ccgcgaccga
      121 tcacgtcgcg cgcgtcacga ccgggaactt cctcggcgag acgcgccgca aggcgtcgaa
      181 cctgaccgac gcggtggcgt cgctggcggg tcatcctcgc gtcgaggacg tgcgcgtgcg
      241 cggcttgatc gccggaattg aagtcgacac cgccgaacgc gccgcatctg ccgccgaagc
      301 gatcccgtac gcttcggctg cgggcaacgt ggtgatcgtg tccccgcccg tcgatgtgtc
      361 cgacgcggcg atcgacggcc tcacggccgc gatcatcgcc gcgctccgct gacgcatcgc
      421 cgcgtttatt gtcgagctcg atgcggttgc ggagccagtt gctcaacgaa ccgcggacgg
      481 cggaggcggt gggcgatcag ggaccgggtg ctaccccagg tgcttggaca gcgcttccag
      541 tcgctcttcg acggtgccgc gcagcggctc gtagtcgatg tcgttgtcgt gcatgatccg
      601 gaccatgcgc tcgtcgatat cgcgctggaa gtccgggtcc atcggccgca acccgtccgg
      661 cacgatgtcg aactcgatcg gcaggtagaa cacgtgcgag tactggctgc gcagatgatg
      721 ccgtgcgatc tcgacgcact cgtcccacag ggcgaactcc ttgcggcgct cctgatggtc
      781 gagcacccag ccggcgtagg tcatgacgtc gatcagcgac cggtcaccga tgaagtcgcg
      841 gtgggtcatc tcgttgcggt actgcttcag gaagatcagc gcctcggtct cgaccgtcgc
      901 gtccttgtcc agcttgaagc ccatcgcggc gacctcgcgc gcgacctcgg gcagcagcgg
      961 cagcttcagc tcttgcgcgg ccacttcggc cagcgtggtc tttccggtcg agaacgatcc
     1021 gacaactgcg attcgcggca tagtgccggg cagtctaccc gcgcacgttc gcgttcgcgg
     1081 cttcgtgtgc cagcagccag cgcttcgcgg cgagcccgcc gccgtaaccg ccgatgccgc
     1141 cgcccgttcg caggatgcgg tggcacggca cgacgaccgg gatcgggttc gccccgcatg
     1201 ccgtgccggc cgcacgcgtc gcgctcggcc ggcccgcgcg cgcggcgagc tcggcgtagc
     1261 tgaccggctc gcccggcggg atcgcgcgca tcgcgtccca gacggccagc cggaacgcgg
     1321 tgccgcgcgg cgagaccggg atcgcgtcga tcgcgcgcag gtcgccgtcg aagtaggcgc
     1381 ggacgcgttt gagtgcctcg ctctcggacg gcggcgagga cgcaggaccg tagatcgcgg
     1441 ccagcggcgt ctcgttccag gcgccgtcga acgcgacgtg caccagagcg tccgcggcct
     1501 gtgccagcgt cagcgttccg atcggcgtcg cgatctcttc cacgatcatc gtcatcgcgg
     1561 ctccttgtcc gaaagcgatc tccatagatg catcgcggcg taggaacgcc agggccgcca
     1621 gcgctccgcc gcggccgcca gcgcgcgcgc ggagacggcc gagccctggc cgaacgcctt
     1681 gcgcaggccc agatccgcgg cgggaaacgc gtccgggtcc ccacccgcgc gcatcgcgac
     1741 gtagtgcgcc gtccattcgc cgatgcccgg cagcgcggtc agcgtgccga tcagcccgtc
     1801 cgaagagtcg atgcggaccc tgccgtcgtc aacggcccga gccagcgcgc ggatggcacg
     1861 ggcttgcgcg ccgcgcaacc cggccgcctc gacctcggcg ttcgcgagcg tggcgggcga
     1921 cgggaacaga tgggtcagcc cctcccccag gtccgtctcg acgggcttgc cgaacagatc
     1981 gacgagcctg gatgcgaccg tggttgcccc ggcgaccgac acctgctgtc cgacgatcgc
     2041 gcggaccgcg acctcgaagc cgtcccacgc tcccggcaca cgcaggcccg gccgcgcgtt
     2101 caccagatgc cgcaacgcgt cgtcggccgc gagcacctcg tcgatgcgca tcgggtcggc
     2161 acccagatcg gcgatgcgcc gtgcgcgctc cacgaccgac agcagccgcg cgggcgacgc
     2221 ggtcgtgacg gttagcagga gatacggctc gcccggagca tggcgaatct cgatggagcc
     2281 gggcgctccg tcggcaagga tcgttcgccg atacgcatcc gcggtggcgg cctcgacgcc
     2341 tcgcgtggcg cgcgtcgcca gaaacccgat cagtgactcc cagtccagcg gcgggcggta
     2401 cgccagccgc atcgtgatcg cgcccgccgg tccgcggcgg ccggagcgac gaacctggct
     2461 cggcggacgc ttgaacgtta aggcgaagtc gtggttgaag gagcggatgc tgccgtagcc
     2521 ggccgcgaac gcgatctcgc ccacgggccg gtcggtctcg tcgagcatgc gcttggcgaa
     2581 gtgcacgcgt cgcgcgcggc cgaccgcgag cggcgacgca ccgacgtgcg actcgaacag
     2641 ccgccgcagc tgccgcgctc cgacgccggc caggcgagcc agcccgtcca cgccgccgtc
     2701 gtccagcgcg ccgccggcga tcagcgacag cgctcgcgat accggctcgg gcaccgccgg
     2761 ccaggcggga atgccggccg aatgctcggg cttgcagcgc aggcacggcc ggaagccggc
     2821 cgcctcggcc gctgccgcgg acggatagaa acgcacgttg cgttgcttgg gcgtgggcgc
     2881 ggggcagacc ggccggcagt acaccccggt ggtgagcacg ccggtgaaga accgcccgtc
     2941 gaagcgccgg tcgcgcgaca gcagcgcccg atagcagcgg tccccgtcca gatccatgcg
     3001 tcagttgtac gccatcgagt gctcccccgc tggcaagaat cggacgcgat cgacaaaaca
     3061 gaatggacta gtcacctcgg gccatcttcg ttttgacccc gaatgcgggc aggaggccgt
     3121 cgttctgacc ctcgaggaca acatgggagg acgttcatgc gcatcaaagc ttccaagctc
     3181 gtcgtcgcag gcctgatcgc cgcggccatc gtcaccgcca accccgccgg agccgtcgag
     3241 ccctgcccgg cggtcgcgtg tgtggtcgtc aacggaaccg tcgtcgcgcc gcacggcgag
     3301 cgcgtcgccg actacaccgt gatcgcgcag cgcgccgacg gctaccacaa gaccagcaag
     3361 agcaacgcca ccggggcgtt caccttcacg ctgccgctgc ccacgccgaa ccagtgctat
     3421 cagatcgcag gcatgtccga tccgttctac acggtgtcga acgtgccgca gaagcaatgc
     3481 tcgtcgagca ccgtcacgct gcacccgagg taccgcatca acggcctcag cggccagcag
     3541 aaggtctatc tcggcagcgc ggccgagaac gtcgagatcc ccgtcgaggt cagcgcactg
     3601 tcgcgcacct atcccgcgcc gttcgccaac gatccgctgc cgtacgtgtt ttcgcaccac
     3661 cacccggacg acaaggtctc ggggctggac gatcatgtgc accacggagc gaccgggttc
     3721 ttcgatccgc cggtcgtgcg caagatcgcc gacggcgtgt ggcagtaccg ctggatcgac
     3781 tcgatcaagc ttcccggcgg ccagcccggc tattacgaca tggactgggg ccgcggcacg
     3841 agcgtgttca acccgatgat ggagtgccgc atgatctggt tcggctacgg catctccaag
     3901 tcctcgcccg ccaaggcgac gccgggaacg atcgtgacga tcgacggcca gcgtctgggc
     3961 actgaagccg gcgcggtcgt gatcaagggc agcggccagg tcaccaccat cagcggctcc
     4021 tcgatcctgg agtggacccc gacgcgcgtg aagttcatcg tgccgccgac ggccaagtcc
     4081 ggctgggtcg aggtcgtgcc gccctcggga gtcccgacga acgcccagcc tctgggcatc
     4141 ggcgtctgaa gcgaaacgaa gcgaaacgaa tccggcaaac gctcgtttat gacgacaacg
     4201 gagaagagcc gatgatcaag atcatgcgtc ccagcctgct gtcccggccg cgcaacgtgg
     4261 ccgagcgctt cccttgcccg gatcccgcga agccgcgggc cgtctgggcg atcccgggct
     4321 tcgacgtgaa ctgcggggtc gcgcgtccgc gcggctggca gcgtccggtc acccctccct
     4381 ctccaaagct gttcatcggc agcggttcgc gatgccgaaa cgccacgggc tcgcttcccg
     4441 cggcttctcc ggctcggagc gcattagaga aggcgatccg agccggacac ccgagcgtcg
     4501 ggaccggctg gccgtcggtg cagcgtcccg attcgcgatg cctgaagcga atcgcgccga
     4561 ggctccctcg tcgtgcgcgt ccgaaacgcc gcactcgcgc cgcctgaccc cctcggatgg
     4621 ggctatcggc ccgcggccgg cgcgcctagg gtcgaggcat gcatctggtt ctcggtcctg
     4681 cggcattgct cgtgatcgcg gtgatcacgg tcggcgtggt ccaggaaggc ggcgcgcccc
     4741 ttggctggga cgtggccggc ttggtgttca cggccgcggt gctgttcggg attccgctcg
     4801 cgatcatgcg ctccgcgtgg gtacaagctc gcggaaagcg acccatcgag cagcatccgg
     4861 tggcacggtc ggagtcaccg tggcgcgccg cgcatccgcg ggcggaagaa gcgccgccac
     4921 gcaaggcggc gtaggcgcga tctagccttc gaccaggtcg ccgtcgatcc ggccgacctg
     4981 cacgcccatc tcttcgagat aggccacgcc ggccgcgatc gcatccgcat cgcccgcgac
     5041 ctcgaggatc acccagccga cctcgtcttc gacgttggca cgacggatgt tggtgacgat
     5101 ctcgaacttg cgaccgaggt tgtatatgac cggctcttgg atgagccgtt ccggaaacgt
     5161 caggtgcaac cgctgctttc ccacgctcac cttctatccg cgagccggct ggatccgcaa
     5221 cagcgtacgg ttcatcgacc cgctcgaaaa gccctgcagg tccagcgtca cgtaggcgaa
     5281 gcccagctcg gccaagccct cggcgagcgt ctcgcgcgcc ggcgaggacg ccagccgcgg
     5341 gatctcgtcc accggcacct cgatgcgcgc cagctcgccg tggtcgcgga cgcgaagctc
     5401 acgcaccccc atcgagcgca gcagcgcttc ggcccgtccg acgcgatcga gccgctcggc
     5461 ggtgacctcg acgccgtagg cgatgcgcga tgccaggcac gccgaggcgg gcttgtccca
     5521 ggtcggcagg ccgcgctcgc gcgacaacgc gcggatctcg tccttgccca ggcccgcctc
     5581 cagcagcggg gcccgcacgc cgcgctcacg ggcggcgcgc tgcccgggac ggaagtcgcc
     5641 ggtgtcgtcg aggttggttc cgaccagcac ggcctccacg ccgagcgacg ggccgagctc
     5701 gtcgagccgt tcgaacagct ccgtcttgca gtggtagcag cggtcgggct cgttcgcgcg
     5761 atactcggcg cgttcgatct cgcgagtctc gatctcgatg tgtcgccagc cgcgctgccg
     5821 cgcgagcgcc gcggcggctt cgcgctccga cgacgcgagc gactcgctca cggcggtgac
     5881 cgccacgcat cggtcggcgc cgtggatctc gtgggcgacg tcggccagga acgcgctgtc
     5941 cgcgccgccg ctgtaggcga ccgcgcacga cccgatttcg gagatgatct cgcgcagacg
     6001 gtcgatgctc atggctcgat tctaggcgtc ccgcgacacc gcggtctcgg ccagcacccg
     6061 gagcgcctgt ttgacgtcgg agatcagcgg cgcggcttcc agctcggcca gcgccttggc
     6121 gtgcagctcg tcgatcagcg ccagggtcgc atccagcgcg ccggaggagc gcagcacgtc
     6181 acggacacgc tcgatatcgg catcggtcag gtccggcgag ccgagcgccg cgtcgaggaa
     6241 cgtccggtcc tccgccgacg cctgcgcgcg cgccttggcg accagcacgg ttcgcttgcc
     6301 ttcacgcagg tcgccttcgg cgtccttgcc ggtgaccgac ggctcgccga aggcgccgag
     6361 cacgtcatcg cggatctgga acgcctcgcc gagcggcgcg ccgaaggcgg tcagcgcctc
     6421 gatcacctgc ggggtcgcgc ccgcgagcgc cgcgccgatc acgaggggct tctcgaccgt
     6481 gtaggcgccg gacttcagcg cggcgatgcg acgggcggcg ggctcgtcgg cctcgcggcg
     6541 ctcggcggcg acgaggtcgg cgaactggcc ggcgatcacc tcgagccgca tgcgcgtgaa
     6601 ccagtcgaac gccgcggaca gacgcaccgc gtcgaagccg gcctcgagca gcaaccggtc
     6661 ggcgagcgcg aatgccagat cgccggtcag gatcgcggcc gaggttccga agcggccgga
     6721 tcccattgcc acgtgcgagg ccgcgaggcc gcgtcgcagc ggggagccgt ccatgatgtc
     6781 gtcgtgcacg atcgcgaacg tgtgcagcag ctcgagcgcc gtgccggcac gcacgatcgc
     6841 atccgagtcc tcgccgccgg ccgcgcgatg accccagtag caaaagaggg ggcgcagccg
     6901 cttgccgccc gcgcccacga cacgcgacag ctcgtcgatc atcggcagcg cggccggatc
     6961 ggccgcggcg acgtcgacgc gaactgcgga caggaagccc gtgagcgcct cgtcgatccg
     7021 ggcgcgaagc tcgttcgggg aaggtgtcat gcgcctttag tctcggcggc gcggcgcggt
     7081 gcaggcaact ccggcaacct taggtgatgc ttaccccgat ggcgctgccg atcccgaagg
     7141 tggtggccgc cgcggccgaa ccgatcgcca gcatgcgcaa cccgctgtgc atcggtcggc
     7201 gtccggtgaa gatcgccagc gccgacccga cgaagaacag cgacaggccg ctcgcgatca
     7261 ccgacgacca aaccgccgcg gccccggtcg cgaataggta ggggaccacc ggcacgaacg
     7321 cgcccagcga gaacgccagg aacgacgaga tcgacgcgac ccacggcgag gcgacatcgt
     7381 caggcgagaa ccccagctct tcacgggtca aagtctcgag cgcgacctcc ggccgggcca
     7441 tgatctcgcg cgcgaccgtc gccgcggtgt cgggcgacag ccccttggcc cggaagatca
     7501 gcgcgagctc ctcctcctct tcctctggga acgcctcgag ctcaccccgc tcgacctcga
     7561 cctctcgctc gtagagctcg gtctgggacc ggaccgagat ccactcgccc gccgccatcg
     7621 agaacgcgcc cgcgagcagc cccgccacgc ccgcgagaag cacgatcttg gcgttgccgg
     7681 ttccgcccgc cacgcccacc acgagcgata ggttcgacac gagcccgtcg ttgacgccga
     7741 acacgccggc tcgcagcgcg ccgccggcgc ccacgcgatg gcgtccctcg cgcgccgcga
     7801 tgcggtcggc ccggtcctgg gcgatggccg cgacggtctg gccgtggccg cgctcctcgg
     7861 cggccatgcc ttcggccgcg tccgcggccg cgtcgtactt ggccgcgtcg gccgattcgg
     7921 cgcgcagcac gaccggcaag accgactcga cgccgaacgt ccgcgcgacc cagccgagca
     7981 ccttcacgcg catcccgacc cgcggcggga gaggctcgac gccggcgagc cgcagcaggg
     8041 actcccaatg agcggcatgc ttctcctcga acccggcgag cttggagaaa acctcgcgcc
     8101 gtgcttcgga gtccgcggcc gcggccagcg cccggtacag ggctgcgccc gcgagctcgt
     8161 cctcgtagta ggcggtccat cgcctgatct gatccggggt ggcccgggag ccgtccggtg
     8221 ggggcgcggg ctgcggagtc gccataccgt cattctgccg gttcgaaccg agccccgccc
     8281 gtcgctatca tgtgcccccg ggaagccatg acccgctttc ggaaactcgc gctcgcgacg
     8341 accgccacga cctacctgct gatcgcagcc ggcgggctcg tgcgcgcaac aggctccggc
     8401 ttgggttgcc cgacgtggcc cggatgctat cccggccggt tcttcccgcc gctcgagaag
     8461 gccgccatga tcgagtggac ccaccgaacg atcgtggtgt tcctgacgat catgatcgcc
     8521 gcgatgacgg tttgggcgct gcgccggttc cgcgccgagc ggcccgtgat ggtcccctcg
     8581 gtggccgcgt tcgggttggt gctgttccag gcggccctcg gcgcgatcac ggtcgagcgc
     8641 gagctcgacc cggtcgtggt caccgcgcac ttcgccactg cgatggtcct ggtcgggacc
     8701 ctgatctccg cgacgatcgc caccttccgc cttcgcgagg tcaccggacc gcgcgttcag
     8761 gcagacttcg cccagctcgc cctcgtcgcc gccgccggga cgttcatcct gttgctggtc
     8821 ggcgcctacg tccggggcct gggcgccggg ctggccttcc cggactggcc gctgatgaac
     8881 ggacgtctgc ttccgcaggc cggccggatc gagaccccgc acttcgtcca ccggctgctg
     8941 gccctgttcg cgggcgcgct ggtcgtcgca ctggcctggc gctcgaaccg cacggcttcg
     9001 cgggacgcgc acccgcgtcc ggttcgcctc ctcgccctgg cggccgcggg cctgtacctg
     9061 gtccaggcct tcgtcggcgc cctgaacgtg tggaccgact tgtccgagcc cacgatcgtc
     9121 gctcacgtgg cgctggcggc cctgtcctgg ggcgcggtgc ttggtcttgc ggtcgtgtct
     9181 cggggcttga gcaggcaaaa cgagcaggac gttgacgcca cgagccccga acgggcggcc
     9241 tcgggccgtg gcctgggccg cgccaaggcc gtcgtggcga cctacttcag gctgacgaag
     9301 ccgcggatca tcgtcctgct gctgatcacc accgtgccca cgatgatggt tgccttccgc
     9361 ggctggccgg gcctggggct cgtgcttgcg acgctgttgg gcggatcgct ggccgcgggc
     9421 ggcgcgaacg ccatcaactg ctatgtcgac cgcgacattg acgccaagat gggccggacc
     9481 tcccgccgtc cgcttccggc cggcgaggtc acgcccgcca gggcgctgga gttcggcatc
     9541 gcgctcggga tcgtgtcctt cgcctacctg tggacattcg tgaacctgct ctcggcggcg
     9601 ctcgcgctgt cggccctgct gttctacgtg ttcgtctaca cgatcggtct caagcgctcg
     9661 actccccaga acatcgtgat cgggggcgcc gcgggcgccg tgccggtcct ggttggctgg
     9721 gcggcggtca ccgggacgct gacctgggct ccggtcgtga tgttcctgat cgtcttcttc
     9781 tggacgccgc cgcacttctg ggcgctggcg ctgcggttca gcggcgacta cgccaaggcc
     9841 ggggtcccga tgctgccggt ggtggccggc cggcgcgaaa cgctcaagca gatccttctc
     9901 tatacggtcg tgctggtggc ggtgaccctg gcgctgaccc cgatcggcga catgggcatg
     9961 gtctatctgg ccgccgccgc cgccctgggc gcctggttca tccgcggggc ggcgatcctg
    10021 tggcgccggg acaccaatca ggcggcctgg tcgctgttca agctctcgat caactatctc
    10081 gccttgctgt tcgtcgcgat ggccgtcgac tcggtcgtcc acgttgccgt cgcatgagcc
    10141 ccttccccgc cgcggccctc cctctcccga gggaaaatgc ggccgagggg ttctcaaacg
    10201 cacacggacc gtgttggtgc ccgcccggga cgtgtggtac ctattgggcc gcgaaacaag
    10261 ggcgtctcac ggtcggacct cgggtcttcc ccccgcattc tcacggagtg acatgacgac
    10321 gaagcgcgcg cggagaatcg taaaggtgct ggcgggcctg gcggccttgg gtctgctcgc
    10381 cgcctgcagc aacgtcggac cgcaggatta cctgcatccc gaaggccccg tcgccgagca
    10441 ggccgacagc ctcttcaagc cggtcttttg gatcgcgacg gcgatcttct tcgtcgtcga
    10501 gggcctgatg gtctacgcga tcttcaagtt ccgcgagcgg cgcggcggga gcgaccctgt
    10561 gcaggtccac ggcaacacca agctcgagat cggctggacg atcgcgcccg cggtgctgct
    10621 ggcggcactc gccgttccca ccgtcgcgat gatcttcgat caggcagccg ttcccgccga
    10681 aggcgaagac gtggtgcgcg tcaaggtcat cggacaccag tggtggtggg agtacgaata
    10741 caccgacgtc gatccgccgg tgaagaccgc gaacgagctg gtcatcccgg tcggcaagaa
    10801 ggttttcctc gaggtcacca gcgcggacgt gatccactcg ttttgggttc cgaagctggg
    10861 cggcaagcag gacgcgatcc cgggccgcat gaacagcatg acgctcatcg ccgacaagcc
    10921 cgacgtctac cacggtcaat gcgccgagtt ctgcgcgctc tcgcacgcga acatgcgcct
    10981 gcgcgcccgc gcgctgccgc aggccgagta cgacgcatgg ctcgccgaac agcagaagga
    11041 cgcggtcgtc ccgacctcgg gcgccgccgc cgagggcgcg cgcgcgttca aggagttcca
    11101 gaacggcacc tgcatggcgt gccacaccat tcgcggcatg gaaggcgcca gcggcaccgt
    11161 cggtccggac ctaacccact tcgccgaccg gctgaccttc gccggctcga tcttcgacat
    11221 gaacgaagag aacctcgcca agtggctgcg cgacccaccg ggcgtcaagc ccgggtcgat
    11281 catgccgaac tatcacatcc ctgaagagac gatcgcggcc atggtcgcct tcttgctgag
    11341 cctcaagtag gagtcgttga tgagttccgt cgccgtatcc agtcgcagca aaggactgtt
    11401 cgcgcggcca tcccacccga aggggatctg ggactggctc acgacggtcg accacaagaa
    11461 gatcgggatt ctctacggcg tcaccgcctt catcttcttt ctcatcggcg ggctcgaggc
    11521 gctgctgatc cgtctgcagc tcgccagccc cgacggcacc gtcctgtccg ccgacgccta
    11581 caaccagatc ttcacgatgc acggcctcac gatgatcttc ctggtcgtga tgccgatgtc
    11641 ggccgccttc atcaactatc tggtcccgct gcagatcggc gcgcgcgacg tggcgttccc
    11701 gcggatgaac gccttctcgt actgggtgtt cctgttcggc ggactgttcc tgtac
//
LOCUS       DATLFO010000376        10218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_69179, whole genome shotgun sequence.
ACCESSION   DATLFO010000376 DATLFO010000000
VERSION     DATLFO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69179"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="VM841_15480"
     CDS             complement(<1..1943)
                     /locus_tag="VM841_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVL91623.1"
                     /translation="MGVNHDKDKPWLFRTYAGHSSAAASNELYKRNLSKGQTGLSVAF
                     DLPTQCGYDADANMARGEVGKVGVPVGHIGDMRALFDGIPLEQMNTSMTINATAMWLL
                     SLYAAAAEEQGAPRAALQGTTQNDILKEYLSRGTYVFPPTASIRLITDMITFCAAEMP
                     KWNPINVCSYHLQEAGAEPHQEVAYTLANAFAYLDAVRSSGRIPESDFGRVVGRISFF
                     CNAGVRFIEETCKMRAFTRMWDRLTEQRYGVTDPKYRRFRYGVQVNSLGLTAVQPENN
                     VYRILLEALAVTLSKDARARALQLPAWNEALGLPRPWDQQWSLRLQQILAYETDVLEY
                     DDIFAGSHVIEAKTAEIEDAAQAELDRLLGMGGAVEAIEEMKEGLVASLAKRRRAIES
                     GEIPVVGANIYPETEPSPLAGTEQMIEKVDAEAEARQLESLASFRAARDEAAATRALD
                     ALRRAAADGSENLMPHSIEAARAGVTTGEWADALREAFGEYRAPTGVSSAALTMNGHD
                     TLKSVRDKVASVAEQIGAGRLRMLVGKPGLDGHSNGAEQIAVRARDAGFEVVYQGIRL
                     TPEQIAVAAADEDVHVIGLSILSGGHDLLVPEVLRQLAERGVDPAKVPVVVGGIIPDE
                     DAKRLLEAGVAKIYTPVDYDLTR"
     gene            complement(1980..2930)
                     /locus_tag="VM841_15485"
     CDS             complement(1980..2930)
                     /locus_tag="VM841_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVL91624.1"
                     /translation="MNSASVRPVDGVEILIVMDNTIDVLMAPQDGVERFSLAGRRIDQ
                     DQPVAEHGFSALVTVIERGSRTSVLYDGGLSPLGLGRNLDVLGIDPRALRALVISHGH
                     FDHHGGLHGLFERHGRAALPLLIHPEAWRRRKVVLPTGAEIALPPPSLTDLEAEGCEV
                     TEETGPSLLLDGSVLVSGQVPRVTDFETGFPIHFGEAPGGGFEPDPLIIDDQNLIVNV
                     RDRGLVIVSGCSHAGAVNVLRNAVGLTGEPRVAGFVGGFHLTGAVFEPLIEPTIAAFA
                     AAGVARVVPAHCTGWKAVHRLAATMPGGFVMPSVGTTIRF"
     gene            3085..3825
                     /locus_tag="VM841_15490"
     CDS             3085..3825
                     /locus_tag="VM841_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91625.1"
                     /translation="MSVRRRLIVIAGSLAALAVVALYPRTDVPSRVDLLAHGYSYGTG
                     YAASCPTSLSLGVTSHKVVSGSPMSLNGQLLAEPGFSVSGQPIQFYQAVFSSTSFTQI
                     SGAVTDSTGSYVKVGWPSLNATYKTKWGPLGGPPTACPEVNSGRATVLVYVRVPWLFD
                     RQPVAGQSTLLYGSVRPLHPGQRIHFMYKRLDDRNFRFSSATMDTNSNFFVPFVFDRP
                     GRWYMRVSFYDLDPFHEGNYTAKIFNVR"
     gene            complement(3869..5095)
                     /locus_tag="VM841_15495"
     CDS             complement(3869..5095)
                     /locus_tag="VM841_15495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91626.1"
                     /translation="MTKRILVVCDGPVPHARMNYRGEPLVAYLGVATDYEVDVVCPRP
                     DAPLPLRHPRIRYHYIAPYKRVKSFSGAVSRAADIARLARVVHKIAVRRKVHCVRSIS
                     SIPTAAALLARRGRGFPVVANLSDFYTDLYASSNLPLKRGVGAAIQKVNTFAARADGV
                     IVDSKEQRDAWVEAGVDPSRCYSLPHGIPRTLLLSPHAPVEDGGPHIPILPAPMLLYV
                     GDISQLDGVDLLFRAIAKLRSQGITLSCLILGQGSDAYKAELSRLSQKLDLAKEIVHV
                     PSVPNRALPELMSRADVLVAPFRLTATTTTAIPNKLLEYLSTDRPIVITATPTLRRTV
                     GSAVAFVAPEDVEGLAAALRGCVEEPSTRGGDLSVRRSLRERLSWVSIMEVERAIIDG
                     FAEGRPPDPADIDFRW"
     gene            complement(5092..5829)
                     /locus_tag="VM841_15500"
     CDS             complement(5092..5829)
                     /locus_tag="VM841_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL91627.1"
                     /translation="MSGSRLIAALKRGSHRDPWKRGEYRRLAAAVTPWVQSGSLVDVG
                     CGEGLFTSFLAEPDSRTTTVVGMDIDSHPDWKMHGESVRFVVGDASRPPMPGGSAEVV
                     LAKDLLHHAEDPAAAATWLVRMAKQRVIVIEANADNPIMAFYTRHNGDQHFTDARLTE
                     LLELACPGAEWQRSAVVSYPFYLPPVRTAAALWVWPVTLLMLLMFKVARSAAGANWLH
                     GRLARLRWSEPFMVRVLDISPSKDARQ"
     gene            complement(5819..9283)
                     /locus_tag="VM841_15505"
     CDS             complement(5819..9283)
                     /locus_tag="VM841_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91628.1"
                     /translation="MDILIVLVLAAFLAVLMTRGILTRPGLIQYSADFSIHAEIAQEL
                     GHWRGAWNPFLGDDNSAVISQAPPFLLLVPLVGTEWAQKLLLLGVYFLMAAAAGLTAY
                     SWIRSLAPRRALILAVSAAIVYTVNPWVSVESVHLYYLLLYALLPLTVHWTRAALTSE
                     TNSGAFRGSVGAGLIVAATLTAYGILFHSMLVGILGLSFLASGPAKFRDRAKLILVGA
                     SGYSLGLIIASMYWILPVILGFRAGFSSGTSWALFTTKDLFVLSPFTPLWLNIAGFYR
                     DTTSFLINTAAGADVASIALVGGYGLLLGVVLFFVVRSTRRRWAEGGLVFSVFFFAWL
                     AKGTGFPRGDAYAALAAAPGLRRIAFILFKGPYKLIPLTVFCLVTLSILALAAIDLRS
                     VAGRVGAVVMSGSIVVWSMVGGLPLLTGDLAGYMAPVSMPEQFRQSLVRSGLGDSKTP
                     GRAVWLPFQRDGNVPPSWIDRRAEIPLISGAAAPLPGWVSPANVSSRGVSWLGPAQGR
                     TLDLIVSELIDGESPDDLGRFLSANGRSAVVVRLDFPGGRETGEKLARRTDFRQRYAD
                     DLLAVYEPRSLSTLPARSEGVTVVVGGIRQIAAESARSEGPLRRPYVLSAHLPEESRS
                     LSWWRAVRQVTFAPGRGWLDLALDSPSDSVTHLSAADLVHQTVPLSEWDKDFFESNTW
                     LPSRLRGMQGRLQDPSLSSNFLVTSANAHLTVPIERAEPCSCQIWVRAFVGPRGGSVI
                     ASTNSGESVTHSMLDEISAGWRWLRGPKVELSPNRHHVEIAFVGGFNAVDVVAVAPVG
                     AAEARAEQLRDALSRTDIVVDSGAAIREGVPVALESIASVIAQSGEVAERRSPGRIAL
                     TGRAHPSSGLWSAVLKPATPITVRGMRGLRLHMSLPEVGDASEIEIAASDGATAPARA
                     SFSMSPGDRVFHVDLGGRPEPAERDFAIDSIVLNIRARPGASVAADLSGIEMIPGGMG
                     SKESVRQVSLPERGRYRVAIRSRVAGNTKAKIASSDLELRRFENSGWLVSDHIELSQG
                     ETSLALPAALDGEATALLYLSRTESRDAFGDAGTLPQGGARIVERIPEPYSPHWVAKT
                     GNRTIEPFRVNGFSTAFIYNDDAPIAVSFAPDRWILPGRLSTLLGLLMLAGYAVRTRS
                     SRKDLHVG"
     gene            complement(9352..>10218)
                     /locus_tag="VM841_15510"
     CDS             complement(9352..>10218)
                     /locus_tag="VM841_15510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVL91629.1"
                     /translation="LLLRRLRRRPTITTIHGVLPLEAWSDALVQAYAGRAPKSIIRWI
                     YGRLIEKMASLSADVIMHGASLERSLRQYGVRGRVSVIPQGIEGQLAPLSRSEARRIL
                     GFDDRPRVLFFGYLLPYKGLDTLRAAAPALTQAGVEVVVAGSDTGDDRPTHRVLAQDW
                     GPIRRMGYVAEELIPALLSAADVMVLPHRIGLSTSGPFSLAVAYGLPVVVSDVPTLAD
                     DLSFPDATFVAGSHESLTTTILRVLNNASVRARLEDRLAEIRAARGWSSVAAATMSRY
                     RDVISSEEVRLR"
BASE COUNT         1936 a         3395 c         3277 g         1610 t
ORIGIN      
        1 atccgcgtca ggtcgtagtc aaccggcgtg tagatcttcg ctacgcccgc ctccagcagc
       61 cgcttcgcat cctcgtcggg gatgatgccg ccgacgacca ccggaacctt cgccggatcc
      121 acgccgcgct ccgcgagttg gcgcagcacc tccgggacga gcagatcgtg gccacccgac
      181 aggatcgaca gcccgatcac atgcacgtcc tcgtccgcgg cggcgacggc gatctgttcg
      241 ggcgtgagcc gaatcccttg atagacgacc tcgaagccgg cgtcgcgcgc gcgcaccgcg
      301 atctgctcgg cgccgttgga gtgtccgtcg aggccgggct tgccgacgag catgcgcagg
      361 cggccggcgc cgatctgctc tgcgaccgac gcgaccttgt cgcgcacgga cttgagcgtg
      421 tcgtggccgt tcatcgtcag cgccgccgac gacacgccgg tgggcgcgcg atactcgccg
      481 aacgcctcgc gcaacgcgtc ggcccactcg cccgtcgtga ccccggcacg cgccgcctcg
      541 atcgaatgcg gcatcaggtt ctcggagccg tcggcggcgg cgcggcgcag cgcgtcgagc
      601 gcacgggtgg cggcggcctc gtcgcgcgcg gcgcggaacg acgcgagcga ctccagttga
      661 cgcgcctcgg cttcggcgtc caccttctcg atcatctgct cggtgccggc cagcggcgag
      721 ggttcggtct cgggatagat gttggcgccc acgacgggaa tctcgcccga ctcgatcgcg
      781 cggcggcgct tggcgagcga agcgacgagg ccctccttca tctcctcgat cgcctcgacc
      841 gccccgccca tgccgagaag ccggtcgagc tcggcctgcg cggcgtcttc gatctcggcg
      901 gttttggcct cgatcacgtg cgagcccgcg aagatgtcgt cgtattcgag cacatcggtc
      961 tcgtaggcga ggatctgctg cagccgcaac gaccactgct ggtcccaggg ccgcggcaag
     1021 ccgagcgcct cgttccacgc gggaagctgc agcgcgcgcg cccgggcgtc cttcgacagc
     1081 gtgaccgcga gcgcctcgag caggatccgg tatacgttgt tctccggctg cacggccgtc
     1141 aggccgagcg agttcacctg cacgccgtag cggaaacgcc ggtatttcgg atcggtcacg
     1201 ccgtagcgct gctcggtgag ccggtcccac atgcgcgtga acgcgcgcat cttgcaggtc
     1261 tcctcgatga aacgaacccc ggcgttgcag aagaacgaga tgcgtccgac gacgcggccg
     1321 aagtcggact cggggatgcg gccggagctg cgaaccgcat ccaggtaggc gaacgcgttc
     1381 gcgagcgtgt aggcgacctc ctggtggggc tccgcgccgg cttcctgcag gtggtaggag
     1441 cacacgttga tcgggttcca cttcggcatc tcggccgcgc agaaggtgat catgtcggtg
     1501 atcagccgga tcgaggcggt cggcgggaac acgtacgtgc cgcgcgacag atactccttg
     1561 aggatgtcgt tctgcgtggt gccctggagc gcggcgcgcg gagcgccctg ctcctcggcg
     1621 gcggccgcat acagcgacaa gagccacatc gcggtcgcgt tgatcgtcat ggacgtgttc
     1681 atctgctcca gcgggatgcc gtcgaacagc gcgcgcatgt cgccgatgtg gccgaccggc
     1741 acgcccacct tgcccacctc gccgcgcgcc atgttcgcgt ccgcgtcgta gccgcactgg
     1801 gtcggcaggt cgaaggcgac gctcaggccc gtctgtcctt tggacaggtt tcgcttatag
     1861 agctcgttgg atgccgcggc cgaggagtgt cccgcgtagg tgcgaaacag ccacggctta
     1921 tccttatcgt ggtttacgcc catgagcgaa tggtagcccg cgagcggcgc ggccgaaaat
     1981 cagaagcgga tcgtggtgcc gacgctcggc atgacgaagc cgccgggcat ggtggcggcc
     2041 aggcggtgga ctgccttcca gccggtgcag tgcgcgggca cgacgcgagc gacgccggcg
     2101 gccgcgaagg ccgcaatcgt cggctcgatc agcggctcga agaccgcgcc ggtcaagtgg
     2161 aagccgccga cgaagccggc gacgcgcggc tcgcccgtga gcccgaccgc gtttcgcagc
     2221 acgttgaccg cgccggcgtg cgagcagccc gacacgatca ccaggcccct gtcccggacg
     2281 ttcacaatca ggttctggtc gtcgatgatc agcgggtcgg gctcgaagcc gccgccgggc
     2341 gcctcgccga agtggatcgg gaacccggtc tcaaagtcgg tgacgcgcgg cacttgcccc
     2401 gagaccagca cgctgccgtc aagaagcagc gacggtcccg tctcctcggt cacctcgcag
     2461 ccttcggcct ccagatcggt cagcgacgga gggggcagcg cgatctcggc cccggtgggc
     2521 aatacgacct tgcgccgccg ccaggcttca gggtggatca gcaggggcag cgcggcccgg
     2581 ccgtgccgct cgaacaggcc gtggagaccg ccgtggtggt cgaagtggcc gtgcgagatc
     2641 accagcgcgc gcagagcccg agggtcgatg cccagcacat ccaggttgcg gcccaggccc
     2701 agcggcgaga ggccaccgtc gtagagcacc gaagtgcgcg agccccgttc gatcaccgtg
     2761 accagggcgg agaagccgtg ctcggcgacc ggctggtcct ggtcgatccg gcgcccggct
     2821 aaggaaaagc gctccacccc gtcttggggg gccatcaaaa cgtcgatcgt gttgtccatc
     2881 acgatcagga tctcgacgcc gtccaccggc cgcacgctcg cactattcat atcgacacct
     2941 tagatatcct ttgcgggcgt gaagcaagcc cgccgggtat gaggggatcg gttgacttgc
     3001 ggccgagcct ggggctatgg tccgaaagtg gtagaacctt acaattggcg tgtgtgtggc
     3061 gacagaccga taagggggtc gtgaatgagc gttcgacgcc ggctgatcgt gatcgcgggg
     3121 agcctggccg ctctcgcggt cgttgctctg tatccgcgta ccgacgttcc gtcccgcgtc
     3181 gatctgctcg cacacggcta cagctacgga accggctatg cggcttcctg cccgaccagc
     3241 ctcagcctcg gggtgacctc gcacaaggtc gtgtccggca gcccgatgtc gctcaacggc
     3301 cagctgctcg ccgagccggg cttctcggtt tcgggccagc ccatccagtt ctatcaggcc
     3361 gtgttcagct cgacatcgtt cacgcagatc tccggagccg tcaccgatag caccggttcc
     3421 tacgtcaagg tcggctggcc ttcgctgaac gcgacgtaca agaccaagtg gggaccgctc
     3481 ggtggtccgc ccaccgcctg ccccgaggtc aacagcggcc gtgccaccgt gctcgtgtac
     3541 gtgcgggtgc cgtggctgtt cgaccgtcag ccagttgccg gccagtccac gttgctgtac
     3601 ggctcggtcc ggccgctcca ccccggtcag cgcatccact tcatgtacaa gcgcctcgac
     3661 gaccgcaact tccgcttctc gagcgcgacc atggacacga acagcaactt cttcgtgccc
     3721 ttcgtgttcg accgccccgg ccgctggtac atgcgagtca gcttctacga cctcgacccg
     3781 ttccacgagg gcaactacac cgccaagatc ttcaacgtca gatagacggg ccatctagag
     3841 gccgcggccg ttggccgcgg cttcttgatt accagcgaaa gtcgatgtcc gcgggatcgg
     3901 gtggcctgcc ctctgcaaaa ccgtcgatga tcgctcgctc gacctccatg atgctgaccc
     3961 aactcagtcg ctccctgagg gaccgacgca ccgaaagatc cccaccccgc gtcgatggct
     4021 cctcgacaca cccacggagt gcggcggcca ggccctcgac atcctccggc gccacgaacg
     4081 cgactgccga gcccaccgtc ctgcgaagcg tgggagtcgc ggtgatcacg atcggccggt
     4141 cggtcgacag gtactccaac agcttgttcg gtatcgccgt ggtcgtggtc gcggtcaagc
     4201 gaaagggcgc aaccaacacg tcggcccgac tcatcagctc cgggagcgca cggttaggga
     4261 ccgacggcac atgaacgatc tccttcgcaa ggtcgagttt ctgcgacagc ctgctcaact
     4321 ctgccttgta cgcgtcggat ccctgtccca ggatcaggca ggaaagggtt attccctggc
     4381 tgcgaagttt cgcgatggca cgaaacagca ggtccacacc atcgagctga ctgatgtcgc
     4441 ctacgtagag gagcatcgga gcaggaagga tcggaatgtg cgggccgcca tcctcgaccg
     4501 gagcgtgcgg ggacagaagc agcgtccggg ggataccgtg gggaagcgaa tagcatcgcg
     4561 agggatcgac cccggcttcg acccacgcgt ccctttgttc cttggaatcc acgatcactc
     4621 cgtcggcacg ggcagcgaag gtattgacct tctggatcgc cgcgccgacc ccacgcttca
     4681 aaggcagatt ggatgacgcg tagagatccg tgtagaagtc gctcaggttc gcgacgaccg
     4741 ggaatccacg tcctcgccgt gcgagcaacg cggccgcggt aggaatcgac gatatcgaac
     4801 gaacgcaatg caccttccgg cggacagcga tcttgtgcac gacccgtgcc agccgagcta
     4861 tatcagcggc tcggctgacg gctccggaga agctcttcac ccgcttgtag ggagcgatgt
     4921 agtggtagcg gattcgtgga tgcctgagcg ggagaggagc gtccggacgg ggacaaacca
     4981 catcgacctc atagtccgtt gcgacgccga gataggcgac gagcggctca ccccgatagt
     5041 tcatcctcgc gtgcggaacc ggcccgtcgc acacgaccaa gatgcgtttg gtcactgccg
     5101 ggcgtccttg gacggcgata tgtcgagaac ccgcaccata aacggctcgc tccagcggag
     5161 ccgcgcgagt cggccgtgaa gccagttcgc cccggcggcg cttcgcgcaa ccttgaacat
     5221 caacagcatc aagagcgtga ccggccagac ccaaagcgcc gcggcggttc ggaccggtgg
     5281 aaggtagaag ggataggaca ccacagcgct tcgctgccac tcagctccgg gacatgccag
     5341 ctccaacagc tctgtcaggc gggcgtcggt gaagtgttga tccccgttgt gacgagtgta
     5401 gaaggccatg atcgggttgt ccgcgttcgc ttcgatgacg atcacacgct gcttggccat
     5461 ccgcaccagc caggtagcgg ccgcggccgg gtcttcggcg tgatgaagca gatccttcgc
     5521 gaggaccacc tcggccgagc ctccgggcat cggaggccgg gacgcgtcac ccaccacgaa
     5581 gcgaacggac tcgccgtgca tcttccaatc ggggtggcta tcgatatcca ttcccaccac
     5641 ggtcgtggtg cgggagtccg gctccgcgag aaaagaagtg aacaagccct caccgcatcc
     5701 gacatcgacc aacgagcctg attgaaccca cggggtgacc gcagcagcga ggcgacgata
     5761 ctcgcctcgc ttccaagggt ccctgtgact cccgcgcttc agggcggcga tcaatctact
     5821 acccgacatg caggtccttt ctcgagctcc gcgtccgaac tgcgtagcct gcgagcatca
     5881 gaagccccaa gagtgtgctc aaccggccgg ggagaatcca tcggtccgga gcgaacgaga
     5941 cggcgatcgg ggcgtcgtcg ttatagatga acgcggtaga gaatccgttg acgcggaagg
     6001 gctcgatggt tcggttacca gtctttgcga cccaatgggg gctgtagggc tcagggatcc
     6061 tctccacgat ccgagcgcct ccctgaggca aagtcccggc gtcgccgaat gcgtcgcgcg
     6121 attcggtgcg cgaaaggtaa aggagcgcgg tggcctctcc gtcgagagcg gcgggaaggg
     6181 cgagagaagt ctcgccttga gagagttcga tgtggtcgct gacgagccat ccggagttct
     6241 cgaaccggcg gagctcaagg tccgaagagg cgatcttcgc tttcgtgttg ccggccaccc
     6301 ggctgcgaat cgcgactcgg tagcgccctc gttcgggtag ggatacctgc cgcaccgact
     6361 ccttcgagcc catcccgccc gggatcatct cgatgcccga aagatccgcg gcgaccgagg
     6421 caccggggcg cgcgcgaatg ttaagaacga tcgaatcgat ggcgaagtca cgctcggccg
     6481 gctccggccg gccaccgagg tccacgtgga aaacccgatc tccgggggac atcgagaagg
     6541 acgctcgagc cggcgccgtc gcaccgtccg aggccgcaat ctcgatctcg gacgcatctc
     6601 caacctctgg caagctcatg tgcaaccgca gtccgcgcat cccacggacg gtgatcggag
     6661 ttgcgggctt gagcaccgcc gaccaaagac cgctcgacgg atgagccctt ccggtcaagg
     6721 caatgcggcc cggactgcgc cgctctgcaa cttcgccgct ctgagcgatg acggacgcga
     6781 tcgattcgag cgcgacggga acgccttctc ggatcgcagc accggaatcg acgacaatgt
     6841 ccgtccggct caacgcatcg cggagctgtt ccgcgcgagc ttccgcggct ccgaccgggg
     6901 ccacggccac cacgtccact gcgttgaacc cacccacgaa cgcaatctcc acgtggtggc
     6961 ggttcggact cagctcgacc ttcggcccac ggagccaccg ccagcctgcc gaaatctcat
     7021 ccaacatcga gtgagtcacc gactcgcccg agttcgtcga agcaatgacg gagccgccac
     7081 gcgggccgac gaacgccctt acccagatct gacacgagca cggctccgct ctttcgatgg
     7141 gcacggtcag atgcgcgttc gcagaggtaa cgaggaagtt gctgctgagc gatggatcct
     7201 gcagcctgcc ttgcatcccc ctcaggcgcg aggggagcca cgtgttgctc tcgaagaaat
     7261 ccttgtccca ctcgctcaaa gggaccgtct gatggacgag gtcggcggcg gagaggtgcg
     7321 tgaccgagtc actcggggaa tcgagtgcaa gatccagcca cccccggccg ggcgcaaagg
     7381 tgacctgtcg aacggcacgc caccacgaca acgatcgcga ctcctcggga agatgcgccg
     7441 aaagtacgta gggccggcga aggggccctt ccgatcgcgc ggactccgcc gcgatctgac
     7501 ggattccgcc aacgacgacg gtcaccccct cggatcgcgc cggcaacgta gacaatgaac
     7561 ggggctcgta cacggccaat agatcgtccg catatcgctg ccggaaatcc gtacggcgcg
     7621 ccagcttctc tccggtttca cgtcctccgg ggaagtccaa gcgaaccacc actgccgaac
     7681 gaccgttggc ggaaaggaac cgccccagat cgtccggcga ctcaccgtca atgagctcgc
     7741 tgacgattag gtcaagcgtt cgcccctgcg ccggcccgag ccatgaaacg ccgcgggagg
     7801 agacattcgc cggagagacc caccccggga gcggcgccgc ggcgccgctg atcaagggga
     7861 tctccgcgcg ccgatcgatc cacgacggcg ggacgttccc atcccgctgg aacggaagcc
     7921 aaaccgcgcg acccggggtc ttcgaatccc cgagaccact gcgtacgagc gattgccgga
     7981 actgctccgg catcgacacg ggagccatgt agcccgcaag gtcacccgta aggagcggca
     8041 ggccgccgac catcgaccaa acgacgatcg aaccagacat gacgacggcc ccgacacggc
     8101 cggcgaccga ccgcagatcg atggctgcca aagcgaggat gctcaaggtc accaggcaga
     8161 agaccgtcag cgggatgagc ttgtacgggc ccttgaacaa gatgaacgcg atccgccgca
     8221 acccgggagc tgctgcaagg gccgcatagg cgtcaccgcg tggaaatcct gttcctttcg
     8281 cgagccaggc gaagaagaac acactgaaga ccaatcctcc ttctgcccat cgtcgacgcg
     8341 tgctccggac cacgaagaag agcacgacgc caaggagaag accgtagccg cccaccagag
     8401 cgatcgaggc cacatccgct ccggcggcgg tgttgatcaa gaacgaggtg gtatcccggt
     8461 agaacccggc gatgttcaac cagagtggcg tgaacgggct caggacgaag agatccttcg
     8521 tcgtgaacag agcccacgac gttccggacg agaatccggc gcggaatcct agtatgaccg
     8581 gaaggatcca atacatgctc gcgatgatca gaccgagcga atacccggag gctcccacga
     8641 ggatcagctt cgcgcggtct cggaacttcg ccggtccgct cgccaagaac gaaagtccga
     8701 ggatccccac gagcatcgaa tgaaacaaga tgccgtaggc cgtgagcgtg gcggccacga
     8761 tgagccccgc tcctacggat ccccgaaagg ccccgctgtt cgtttccgag gtaagcgctg
     8821 ctcgggtcca gtgaaccgtc agtggcagca gcgcgtagag cagcaggtag tacagatgca
     8881 cggattcgac cgacacccat ggatttaccg tgtaaacgat tgcggctgac actgcgagga
     8941 ttagggcacg tcgaggcgca agcgatcgga tccaggaata ggcggtcagt cccgcggcgg
     9001 ccgccatcaa gaagtacacg cccagcagca gcagcttctg cgcccactcg gtcccgacga
     9061 gaggaacgag gaggagaaac ggcggcgcct gactgatgac ggccgagttg tcgtcgccca
     9121 ggaagggatt ccacgccccg cgccagtgcc cgagctcctg cgcgatctcc gcatgaatgc
     9181 tgaaatcggc tgagtactga atcaatccag gccgagtaag gatcccgcgc gtcatcaaca
     9241 ccgctagaaa cgcggcgagc acgagcacga tgaggatgtc caagcgcggc gcccgcgccc
     9301 tcccatgggg ctcggcatga actgcctgcc gaacttcacc cgtcgttgag atcatcgaag
     9361 acgtacctct tcggacgaga tgacgtcccg ataccgcgac atcgtcgctg ctgccaccga
     9421 ggaccatccc cgcgcagccc ggatctcggc aagacggtcc tcgagtcgcg ctcgaaccga
     9481 ggcgttgttc agaacacgca agatcgtcgt ggtcaatgat tcgtgacttc cggcgacgaa
     9541 ggtcgcatcc ggaaaggaaa gatcgtcggc gagcgtaggg acgtcggaaa cgacgaccgg
     9601 gagcccgtag gcgaccgcga gcgagaaggg cccgctcgtt gaaagaccga tccggtgggg
     9661 aaggaccatg acatcggctg cggagagaag cgcgggaatg agctcttcgg ccacatatcc
     9721 catccggcgg atcggacccc aatcctgtgc caagacgcgg tgggtcggcc ggtcgtcgcc
     9781 cgtatcggat ccggccacga cgacctccac cccggcctgc gtcagagcgg gcgcagcggc
     9841 cctcaacgta tcgagtccct tgtagggaag gagatacccg aagaacaaca cccgcggccg
     9901 atcatcgaat ccgaggattc tccgcgcttc ggagcgcgaa aggggcgcca actgtccctc
     9961 gatgccttgc gggatcaccg acacccgccc tcgaaccccg tactgcctga gtgaacgttc
    10021 aagggaggca ccatgcatga tcacatccgc gctcaggctc gccatcttct cgataagacg
    10081 gccatagatc caacggatga tcgatttcgg agcacgaccc gcgtaagcct gaaccaatgc
    10141 atcgctccag gcctcgagcg gcagcacccc gtggatcgtg gtgatcgtcg gccggcgacg
    10201 cagcctgcgg agcagaag
//
LOCUS       DATLFO010000377         4144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_19758, whole genome shotgun sequence.
ACCESSION   DATLFO010000377 DATLFO010000000
VERSION     DATLFO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19758"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..840
                     /gene="ftsE"
                     /locus_tag="VM841_15515"
     CDS             148..840
                     /gene="ftsE"
                     /locus_tag="VM841_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HVL91630.1"
                     /translation="MIKTINVSKVYKNGVTALRDVSVEIFKGEFVFLVGPSGSGKSTF
                     IRLMIREEQPSSGEIYVAGKNIAQLASWKIPMLRRNVGCVFQDFKLLPNRTVFENVAF
                     ALEVIGRPKSQISKQVPEMLDLVGLAEKAHHRPDELSGGEQQRVAVARAFVNRPQILL
                     ADEPTGNLDPATSVEIMKLLDRINRLGTTVLMATHDHAIVDAMRRRVIELEKGGVVRD
                     QERGVYGYGAKN"
     gene            849..1727
                     /gene="ftsX"
                     /locus_tag="VM841_15520"
     CDS             849..1727
                     /gene="ftsX"
                     /locus_tag="VM841_15520"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HVL91631.1"
                     /translation="MAIKLDYAVQETITNIRRNGFMTLASVLVVAVSLYLVGGVLLGR
                     FAINRIIELQTRKVEVAVFLSPEVTEDQRTSIHDDLKLMPEVQDVDYESKDEAYERFK
                     EIFRDQPDIVANTDPEALPESFRVKLRNPEQFEIVNDRLEGRPGILRIQDYRSLLKQF
                     FGVVNDIGRIGLVLVVLLSGAAAMIIGTTIRIAIYARRKEIAIMKLVGASNWFIRIPF
                     MLEGVVHGVLGTIAAVVLLLATRPFFIRVADHIRFLHVNVSNMEVLQYGLWLLVGGVV
                     LGALGSLLGLRRFLEV"
     gene            1915..3144
                     /locus_tag="VM841_15525"
     CDS             1915..3144
                     /locus_tag="VM841_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVL91632.1"
                     /translation="MRPKRTTSIGLITALLAGLLSLTAPPATAADKQPSASRWSELQK
                     KIDQTRKRINEAKKRERGIIAQINASDIRRQALEDKIENLSAQLSDAIAALAALEAAA
                     NRTQMELDLRTRDLEQTIALLEQQQDVLNNRAASKYMAGPMSLAPVLFNTTDFSAFLT
                     ADQYVESVLSADVKVVEEVREVRDEVEVERNAIGARKAELDKQVATAQAERDRIAGIR
                     ASQNRARRAVINEINYRKNLLEKVRDERQAYEEALQSYIRESDSISGILKGAQRGQKV
                     IQGAGKGYLVWPVSGRISSDYGERVHPIYKKKSFHTGIDIAAPQGTKVIAARDGKVVF
                     TGYKGAFGLIVLVDHGDSVATMYSHLSRAYVSAGEKVKRGEGVGAIGCTGWCTGPHLH
                     FEVRVEGEPTNPMRWLK"
     gene            3248..3733
                     /gene="smpB"
                     /locus_tag="VM841_15530"
     CDS             3248..3733
                     /gene="smpB"
                     /locus_tag="VM841_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HVL91633.1"
                     /translation="MAKTKKSENPNIATNRKARHDYHIEETFECGLSLMGSEVKSLRA
                     GKASLADAYAAVRDGEVWLLGAHIPPYLEASYQNHEPTRSRKLLLHRKEIEKIAATTA
                     QKGMTLVPLRLYFVKNKIKCEIGLAIGKKAHDKRQTMIGREAEREMRRREGAVRRGKV
                     R"
     gene            3767..3895
                     /locus_tag="VM841_15535"
                     /pseudo
     CDS             3767..3895
                     /locus_tag="VM841_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          787 a         1350 c         1340 g          667 t
ORIGIN      
        1 aggccggcac cggggcgaag ctcaagcgga tcggcgtcga cggcttcggc gaatccggtg
       61 gcaccgccga actgtacgaa aagtacggtt tgtccagcgg tcatctggtg cgagccgcga
      121 aaggattgct cgggtaacct agcgcggatg atcaagacca ttaacgtctc gaaggtctat
      181 aagaacggcg tcaccgcgct ccgcgacgtc agcgtcgaga tcttcaaagg cgagttcgtc
      241 ttcctggtgg gcccgtccgg gtcgggcaag tcgacgttca tccgcttgat gatccgcgag
      301 gagcagccga gctccggcga gatctacgtg gccggcaaga acatcgcgca gctcgcctcg
      361 tggaagatcc cgatgctgcg gcgcaacgtc ggctgcgtct tccaggactt caagctgctg
      421 cccaaccgca ccgtcttcga gaacgtggcc ttcgcgctcg aggtgatcgg gcggccgaaa
      481 tcccagatct ccaagcaggt gccggaaatg ctcgacctgg tgggtctggc cgagaaggcg
      541 catcaccggc ccgacgagct ctcgggcggc gagcagcagc gggtggccgt cgcgcgcgcg
      601 ttcgtcaacc ggcctcagat ccttctcgcc gacgagccaa ccggaaacct ggacccggcg
      661 accagcgtcg agatcatgaa gctgctcgac cgcatcaacc ggctcggcac gaccgtgctg
      721 atggccaccc acgaccacgc gatcgtcgac gcgatgcgcc ggcgcgtgat cgagctggaa
      781 aagggcggcg tcgtccgcga ccaggagcgc ggcgtctacg gttacggcgc gaagaactag
      841 agacctctat ggcgatcaag ctcgattacg cggttcaaga gaccatcacc aacatccggc
      901 gaaacggctt catgacgctt gcctcggtgc tggtggtcgc ggtctcgctg tatctggtcg
      961 gcggcgtgct gctcggccgt ttcgcgatca accggatcat cgagcttcag acccgcaagg
     1021 tcgaggtcgc ggtgtttttg agccccgagg tcacggagga tcagcgaacg tcgatccacg
     1081 acgatctcaa gctgatgccc gaggttcagg acgtcgatta cgagtccaag gacgaggcgt
     1141 acgagcgctt caaggagatc ttccgcgacc agcccgacat cgtggccaac accgatccgg
     1201 aagcgctgcc cgagtccttc cgcgtaaagc tgcgcaaccc cgagcagttc gagatcgtca
     1261 acgaccggct cgagggccgg ccgggcatct tgcgaatcca ggactaccga tcgctgctca
     1321 agcagttctt cggtgtggtc aacgacatcg gccggatcgg cctggtgctg gtcgtgctgc
     1381 tctccggtgc ggccgcgatg atcatcggca ccacgatccg catcgcgatc tacgcgcggc
     1441 gaaaagagat cgcgatcatg aagctggtgg gggcctcgaa ctggttcatc cggatcccgt
     1501 tcatgctcga gggagtcgtt cacggcgtgc tcggcaccat cgccgccgtc gtgctgctgc
     1561 tggccacgcg tcccttcttc atccgcgtcg ccgaccacat ccgcttcctc catgtgaacg
     1621 tctcgaacat ggaggtcctc caatacgggc tctggctcct tgtgggcggg gtcgtcctcg
     1681 gagccctcgg gtccttgctc ggcttgcgcc gcttcctcga ggtctagccc taggaatcaa
     1741 ccatttgatc cactagggtg tcctttcggc gccctttttg cccttgttga aagggtcgct
     1801 aaagggaaaa gccggtcgcg ccgatgggaa aactgcggtg cgagtgttga cacctgttgc
     1861 gtttggttat gtacgatgca ggtgttccaa cgcctttttg ctgggggagt gctggtgcga
     1921 cccaaacgaa ccacttcgat cggcttgatc acggccctgc ttgccgggct gctgtcgctg
     1981 accgccccgc cggccaccgc cgccgacaag cagccctccg cgagccgctg gtccgagctg
     2041 cagaagaaga tcgaccagac ccgcaagcgc atcaacgagg ccaagaagcg cgagcgcggc
     2101 atcatcgccc agatcaacgc ctccgacatc cgccgtcagg cgctcgagga caagatcgag
     2161 aacctgagcg cccagctgtc cgacgcgatc gcggcgctcg cggcgctgga ggccgcggcc
     2221 aaccggactc agatggagct ggaccttcgc acgcgcgatc tcgagcaaac aatcgcgctg
     2281 ctcgagcagc agcaggacgt tctcaacaac cgggcggcca gcaagtacat ggccgggccg
     2341 atgtcgctcg cgccggtcct gttcaacacg accgacttca gcgcgttcct gacggccgat
     2401 cagtacgttg agagcgtgct cagcgcggac gtgaaggtcg tcgaggaggt gcgcgaggtt
     2461 cgcgacgagg tcgaggtcga gcggaacgcg atcggagcgc gcaaggcgga gctcgacaag
     2521 caggtcgcca ccgcgcaggc cgagcgcgac cgcatcgcgg gcattcgtgc gagccagaac
     2581 cgcgcgcggc gcgccgtgat caacgagatc aactaccgca agaacctgct cgagaaggtc
     2641 cgcgacgagc ggcaggccta cgaagaggcg ttgcagtcct acatccgcga gtcggattcg
     2701 atcagcggca ttctcaaggg cgcgcagcgc ggccagaagg tcatccaggg cgccggcaag
     2761 ggctatctgg tctggccggt cagcgggcgc atctcctcgg actacggcga gcgcgtgcat
     2821 ccgatttaca agaagaagtc gttccacacc ggcatcgaca tcgccgcgcc gcagggaacc
     2881 aaggtcatcg ccgcgcgcga cggcaaggtc gtgttcaccg gctacaaggg cgcgttcggc
     2941 ttgatcgtgc tcgtggacca cggagactcc gtcgcgacga tgtactcgca cctttcgcgg
     3001 gcctacgtaa gcgccggcga gaaggtcaag cgaggcgaag gcgtcggcgc gatcggctgc
     3061 accggctggt gcaccggccc gcaccttcac ttcgaggtcc gtgtcgaagg cgagccgacc
     3121 aacccgatgc gctggctcaa atagacgcag caccccctgc tcgaccctgt accttcggcc
     3181 cgcccacgac ggcgggtcga agcgtttctc gcctccgggg tcgctcgcgg ccgggtagca
     3241 ttcgcccgtg gccaagacca aaaagtccga gaacccgaac atcgcgacca atcgcaaggc
     3301 gcggcacgac taccacatcg aagaaacctt cgaatgtggg ctctcgctca tgggaagcga
     3361 ggtcaagtcg ctgcgcgccg gcaaggcgtc gctcgccgat gcctacgccg ccgtgcgcga
     3421 cggcgaggtc tggctgctgg gcgcgcacat cccgccctac ctcgaggcct cgtaccagaa
     3481 tcacgagccc acccgctcgc gcaagctgct gctgcaccgc aaggagatcg aaaagatcgc
     3541 ggccacgacc gcgcagaaag gcatgacgct ggtgccgctg cgcctgtact tcgtcaagaa
     3601 caagatcaaa tgcgagatcg gcctggcgat cggcaagaag gctcacgaca agcgccagac
     3661 catgatcggc cgcgaagccg aacgcgagat gcgccgccgc gaaggcgccg tccgccgcgg
     3721 caaggtccgc taaggtcagt ttttcgcggt gtagtatgct acacccgtgg agatcaccgt
     3781 tcccgaggcg gccgaacgga ccggtctggc cgccgagacg atccggcgct ggatccgcag
     3841 cggcaagctg cccgcgcgca aggccggcac gcagcacatg atcgaggagt ccgacctcga
     3901 tcggatctcc gcgcagccgc ggcctgcggt cgtctacgag caggcgcccg catacgacga
     3961 cgaaagggct cgtccggggc cgagctcgct gcgattgatg aagcgaatcg tcgcgcatcc
     4021 cgatgtgctc ggcgatgtgg gggtaggcgg cgagcacgtc cggcaccgag tttccggcag
     4081 ccaggaactc aaggatcagc tcaaccgaga tgcgagtgcc acgaatcgcc ggcttgccgc
     4141 cgag
//
LOCUS       DATLFO010000378         5075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_69323, whole genome shotgun sequence.
ACCESSION   DATLFO010000378 DATLFO010000000
VERSION     DATLFO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69323"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VM841_15540"
     CDS             <1..993
                     /locus_tag="VM841_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HVL91634.1"
                     /translation="ECLIASLRLQEGPDAGEMIDLDVPERLGEGTLERGDSIVVVHYT
                     NPEAVDAGVEYQFADRDRKPALIALIVVFAVAVVALGRLRGLAALAGLVASLAILLSF
                     VLPSILEGNSPLLVAVTGASAIAFVALYAAHGINVRTTVALLGTIASLALTALLGTMF
                     SGVARLTGLLSEEVPFLQLAAGRIDFEGIFLAGLVIGALGALDDMTVTQASVVWELRT
                     AGLRDSRELFRSAMRVGRDHVSSTVNTLALAYAGASTTLLLLFVLSGERITQIGNGEI
                     VATEIVRTLVGSIGLVASVPITTMLAVRLSAAPPEAEEPPETAPPADASPSPVE"
     gene            complement(1011..2837)
                     /locus_tag="VM841_15545"
     CDS             complement(1011..2837)
                     /locus_tag="VM841_15545"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HVL91635.1"
                     /translation="MRTVAVKLAVAALSIMVLLAWQMPVSKAQSPAAAGQWLSPFSED
                     FDGDGIGDFDARPPLNRSESASLPPAVNAVVLADGTVLYWSQLEGSESVEHSIAFQQP
                     AGPRSRSRVLDLSSYFASGALPSPAAFATPAPEDGAGGDMVGTDQRHLADGRVLVIGG
                     TRWSNEDPGAPGGFGRTELYGLPDARVFDPLNGTWSRAADMNRPRYYPSMVTLADGRM
                     LVASGADRLVYDSAIFPGPHPSGDVRPANVHEVEVFDPTTGTWTLPSNPESKTALPPF
                     AHLHLMPDGRVLYPGAGMFYPPGGADAEADRWHEDRIYDPASRSWTAAGRPLLGARSN
                     AFTSMMRLTPPYDSAQILVGGGTLGPWPGSHVATTLTEILTWRGGSVERAAARPMTAA
                     RWHASAVTLPTGEVVALGGADKDEVVDPGSGLPVRHAELFDPATRTWRKLARAARDRT
                     LHHTAVLLRDGSILTGGHSPVPAHYGAHTTNVPGLSNNFKDPSFEIFRPPYLFRGERP
                     RITGLSPAAAANGATLRIDTPDAHNATLQVVLSRLPATTHVIDNDQRTVLLGHVIEGD
                     GSVRVTVPDAAVVPPGHYYVFVLKDNGSGPTPSVAEIVRVGS"
     gene            complement(2849..>5075)
                     /locus_tag="VM841_15550"
     CDS             complement(2849..>5075)
                     /locus_tag="VM841_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVL91636.1"
                     /translation="VRTAATRRPATAADGSAGSLSDSAPPAARRAEPSLPAPAGWPFP
                     EVFPRTSGTGRLAGGALLWTDFIYDDRGARSHPVATPVTPLAWPRGTYEYADPDAAAN
                     GADIFRAGIGLDDEATYWRVDWNTLIDPAIPAAMFALDLDGDASTGATRWPADAGIRS
                     AGIERAIFVTSAGAWLYDLAAGTRVPLPAPAVEIEARSFVVRVPMTVLPVWGTWRVRL
                     ASGLATGDGEQFAPVSERHGARRGGPSIYNVGFRTNEQERPVMGTPKSTATMSYWMEH
                     AQAAALAEGDVSAFFADVDWSLLQSRSTTPEPQPRGWSSRWYVSSIELGQGIIRDERE
                     PQDGRPNYLGRVQPYAIFVPSSYAPGRPAPLTWLLHSAFQPHTAFAAMTPAFAEAACE
                     QRGNICVTPLGRGHDGWFFDEAELDFWEVWGSVARAYSLDSERTVIAGYSMGGFGAYR
                     IGLSHPDLFARVAAIAATQICGERFAPGVDHSQHPGPCTTDADTTPLLENARHLPVFI
                     THGTTDELLPFTGALLQARELDRLGYRYRFEHALGQPHIPWYMQDAWFPAAEGLGEPV
                     RESSPARATLRWYPNADRPEWGMAVRGAWWMRELRAARIRPGLTARVDAHSHALPNRT
                     ITPQRATYESLRSDSTPILVEELTWLQGGPRPRRATVTITLENVAAALADLRAAGLRS
                     HEGATLSVTTDEHTTFRFTGLAGTVVVGGTAAGTAVPDAAFEVSLPPGTHTIRIVPPP
                     R"
BASE COUNT          724 a         1704 c         1899 g          748 t
ORIGIN      
        1 gagtgcctca tcgcatcgct gcggctgcag gaaggacccg acgcgggaga gatgatcgat
       61 ctcgatgtgc cggagcggct cggcgaaggc acgctcgagc gaggcgactc gatcgtcgtg
      121 gtgcattaca ccaaccccga agccgtcgac gcgggcgtcg agtatcagtt cgccgatcgc
      181 gaccgcaagc cggcattgat cgcgctgatc gtggtgttcg cggtggcggt ggtcgccttg
      241 ggacggctcc gcgggctcgc ggcgctcgcc ggcttggtgg cgagcctggc catcctgctg
      301 tcgttcgtgc tgccgtcgat cctggaggga aacagcccgt tgctggtggc agtgaccggc
      361 gcgtcggcga tcgctttcgt tgcgctgtat gccgcgcacg gcatcaacgt gcgcaccacg
      421 gtcgcgttgc tcggcaccat cgcgagcctg gccctcacgg cattgctcgg caccatgttc
      481 tcgggcgtgg cccggctgac cgggctgctt tcggaagagg ttccgttcct ccagctcgct
      541 gcggggcgga tcgacttcga ggggatcttc ctcgcggggc tggtgatcgg cgccctcggc
      601 gcgctcgacg acatgaccgt cacgcaggcg tcggtggtgt gggagctgcg caccgccggc
      661 ttgcgggact cgcgcgagct gttccgttcg gcgatgcgcg tgggccgcga tcacgtgtcg
      721 tccaccgtga acacgctggc gctcgcctac gccggcgcct ccacgacgct gctgctgctg
      781 ttcgtgctgt cgggcgagcg catcacgcag atcggcaacg gcgagatcgt cgcgaccgag
      841 atcgtgcgca cgctggtggg cagcatcggt ctggtcgcgt cggttccgat caccacgatg
      901 ctcgcggtcc gcctgagcgc cgccccgccc gaggcggagg aaccaccgga gaccgcgcca
      961 ccggcggacg cttcgccttc gccggtcgag taggccgcgc ttcgtcgggt ctaggagccg
     1021 actcgcacga tctcggcgac cgatggtgtc gggccgctcc cgttgtcctt gagaacgaac
     1081 acgtagtagt ggcccggcgg gacaacggcg gcgtccggca cggtcacccg cacgctcccg
     1141 tcgccctcga tcacgtggcc gagcaggacg gtgcgctgat cgttgtcgat cacgtgcgtg
     1201 gtcgccggca gccgcgacag cacgacctgg agcgtggcgt tgtgcgcatc cggcgtgtcg
     1261 atccgcaggg tggctccgtt cgcggcggcc gccggcgaca ggccggtgat gcgcggccgc
     1321 tcgccgcgga acaggtacgg cggacggaag atctcgaacg aggggtcctt gaagttgttc
     1381 gacagtccgg gaacgttcgt ggtgtgggcg ccgtagtgcg cgggcacggg ggagtggccg
     1441 cccgtcagga tgctcccatc gcgcagaagc acggccgtgt gatggagcgt gcggtcgcgc
     1501 gcggcccgcg cgagctttcg ccacgttcgg gtcgccggat cgaacagctc cgcgtggcgg
     1561 accggcagtc ccgaccccgg atcgacgacc tcgtccttgt ccgcgccgcc gagcgccacg
     1621 acttcgccgg ttggaagcgt gacggcgctt gcgtgccagc gcgcggccgt catcgggcgc
     1681 gccgcagcgc gctcgaccga gccgccgcgc cacgtgagga tctcggtgag cgtggtggcg
     1741 acgtggctgc ccggccaggg accgagcgtg ccgccgccga cgaggatctg cgccgagtcg
     1801 tagggcggcg tcaggcgcat catcgaggtg aaggcgttgc tgcgcgcgcc gagcagcggc
     1861 cgtcccgccg cggtccacga gcgcgaggcc gggtcgtaga tgcggtcctc gtgccaccgg
     1921 tcggcctcgg cgtccgctcc gccgggcgga tagaacatgc ccgcgcccgg gtacaggacg
     1981 cggccgtcgg gcatcagatg caagtgcgcg aagggcggca gggcggtttt gctttcggga
     2041 ttcgacggga gggtccatgt tccggttgtg ggatcgaaga cctcgacctc gtgcacgttc
     2101 gcgggacgca cgtcgccgga cggatgcgga ccggggaaga tcgcgctgtc gtagacgagg
     2161 cggtcggcgc ccgatgccac gagcatgcgg ccgtcggcga gcgtgaccat gctcgggtag
     2221 taacgggggc ggttcatgtc ggcggcgcgc gaccaggttc cgttcagcgg atcgaacacg
     2281 cgcgcgtcgg gcagtccgta cagctccgtg cggccgaacc cgcccggcgc gccgggatcc
     2341 tcgttgctcc accgggtgcc accgatcacc agaacacgcc cgtcggcgag atgccgctgg
     2401 tcggtgccga ccatgtcgcc gccggcgccg tcttcgggag caggcgtggc aaacgcagcc
     2461 ggtgacggca gcgctccgga cgcgaagtag gacgacaggt cgagcacccg cgagcggctg
     2521 cgcggtccgg ccggctgctg gaacgcgatc gagtgctcca ccgactcgct tccctcgagc
     2581 tggctccagt acaggacggt gccgtcggca agcacgaccg cgttcaccgc cggcggcagc
     2641 gacgccgatt cggagcgatt gagcggcggc cgcgcgtcga aatctccgat gccgtcgccg
     2701 tcgaagtcct cggagaacgg cgagagccac tgtccggcgg cggcaggaga ttgcgctttc
     2761 gagaccggca tctgccaggc gagcagcacc atgatggaaa gagcggcgac ggcgagcttg
     2821 acggcgacgg tgcgcatggc ggactcctct acctcggggg cgggacgata cggatcgtgt
     2881 gcgtaccagg tggaagcgat acctcgaacg cggcatccgg gacggcggtg cctgcggccg
     2941 tgccacccac cacgacggtg ccggcgaggc ccgtaaagcg gaacgtggtg tgctcgtcgg
     3001 tcgtgaccga cagggtcgcg ccttcgtgcg aacgcaggcc cgccgcccgc aggtcggcca
     3061 atgcggccgc gacgttctcg agcgtgatcg tgaccgttgc gcggcgtggc cgcgggccgc
     3121 cctgcagcca ggtcagctcc tcaaccagga tcggcgtcga gtccgatcgc agcgactcgt
     3181 aggtcgcgcg ctgcggcgtg atcgtgcggt tgggaagggc gtggctgtgc gcatcgacgc
     3241 gtgcggtgag gccgggccgg atccgtgcgg cacggagctc gcgcatccac caggcgccac
     3301 gcacggccat accccattcg ggacggtccg cattcggata ccagcgcagg gtcgcgcgcg
     3361 ccgggctgct ctcgcgcacc ggctcaccca gaccttccgc ggccgggaac cacgcatcct
     3421 gcatgtacca cgggatgtgc ggctggccga gggcgtgctc gaagcgatag cgatagccga
     3481 gccggtccag ctcgcgggcc tgaagcaggg caccggtgaa cgggagcagc tcgtcggtgg
     3541 tgccgtgcgt gatgaagacg ggaaggtgcc gcgcgttctc gagcagcggc gtggtgtcgg
     3601 cgtcggtggt gcatgggccc ggatgctgcg aatggtcgac gccgggcgcg aaccgctctc
     3661 cgcagatctg tgtcgccgcg atcgccgcga cgcgtgcgaa caggtcggga tgggaaaggc
     3721 cgatgcggta tgcgccgaag ccgcccatcg aatagccggc gatgacggtg cgctccgaat
     3781 ccagcgagta ggcacgcgcg acgcttcccc agacctccca gaagtcgagc tcggcctcgt
     3841 cgaagaacca gccgtcgtgg ccgcggccga gtggggtgac gcagatgttg ccgcgctgct
     3901 cgcacgccgc ctcggcgaaa gcgggagtca tggccgcgaa cgcggtatgc ggctggaacg
     3961 ccgagtgcag cagccaggtc agcggcgccg gccggcccgg cgcgtaggag gagggcacga
     4021 agatcgcgta cggctgcacg cgtcccaggt agtttggacg gccgtcctgc ggctcgcgct
     4081 cgtcacggat gatgccctgg ccgagctcga tcgaggacac gtaccagcgg ctggaccaac
     4141 cgcgcggttg cggctcaggc gtggtcgaac gcgactgcag cagcgaccag tccacgtcgg
     4201 cgaagaacgc cgagacatcc ccttcggcga gcgccgctgc ctgcgcgtgc tccatccagt
     4261 agctcatcgt cgcggtggac ttcggcgttc ccatcaccgg ccgttcctgc tcgttcgtcc
     4321 ggaacccgac gttgtagatc gacggaccgc cgcggcgcgc gccgtggcgt tcggacaccg
     4381 gggcgaactg ctccccatcg ccggttgcga gaccggaggc cagccgcacc cgccacgtcc
     4441 cccacaccgg cagcacggtc atgggtacgc gaacgacgaa cgatcgggct tcgatctcga
     4501 cggccggtgc cggcagcggc acacgcgtcc cggccgccag gtcgtagagc cacgcgccgg
     4561 ccgacgtgac gaagatcgcc cgctcgatgc cggccgaccg gattcccgcg tcggccggcc
     4621 aacgagtcgc gccggtcgag gcgtcaccgt ccaggtcgag cgcgaacatc gcggccggga
     4681 tggccggatc gatcagcgtg ttccagtcca cgcgccagta ggtcgcctcg tcgtcgagcc
     4741 cgatcccggc gcggaagatg tcggcgccgt tggcggcggc gtcggggtcg gcgtactcgt
     4801 acgtgccgcg cggccaggcg agcggcgtga cgggcgtggc gaccgggtgg cttcgcgcgc
     4861 cgcggtcgtc gtagatgaaa tcggtccaca gcagtgcgcc gccggcgagc cggccggtac
     4921 cgctcgtgcg ggggaacacc tcggggaacg gccagccggc cggtgccggc agcgacggtt
     4981 cggcgcgacg agcggccggg ggagcggaat cggagaggct cccggcggat ccgtccgctg
     5041 cggttgcggg gcgacgcgtc gcagcggttc gcacc
//
LOCUS       DATLFO010000379        12986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_147857, whole genome shotgun sequence.
ACCESSION   DATLFO010000379 DATLFO010000000
VERSION     DATLFO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147857"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VM841_15555"
     CDS             complement(<1..318)
                     /locus_tag="VM841_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91637.1"
                     /translation="MRDAIIRLILARHDRPFLPEWAHRLDEAGIARVAAMPASSLDRL
                     LPALSRSANGGRLWMGIAAALAFTGPRERKAALRGMASLSLTSAVVNGVIKLAARRHR
                     PEED"
     gene            382..744
                     /locus_tag="VM841_15560"
     CDS             382..744
                     /locus_tag="VM841_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91638.1"
                     /translation="MPRRSPEQRMMKWMTAGAYTMRHWRGILLSIWLGTVATFGLLAW
                     WGFGNRDSGFGAFVFTVFTLLVIAMVFRYPRRLLRSWRTSRRGPPAVGRPGGVIDAEA
                     DVRDRPRREPPPLGPRRP"
     gene            complement(845..994)
                     /locus_tag="VM841_15565"
     CDS             complement(845..994)
                     /locus_tag="VM841_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91639.1"
                     /translation="MAEEEKKRSKKGIFAVLLAAVGAVFFFLKKKRRNGEETGWEEAT
                     PGGSA"
     gene            complement(1094..1936)
                     /locus_tag="VM841_15570"
     CDS             complement(1094..1936)
                     /locus_tag="VM841_15570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVL91640.1"
                     /translation="MSDSDGIRARSTQSDRVRRVRADRIAANRRGWDDRAARYQQAVG
                     GRDMYAGRIHWGPNKFDDEHLGVIGDVAGLEVLEIGCGAAQFGIELARRGARMTGIDL
                     SGEQILHGRANVKAAGVKMRLITGNAEKLRARFGEGTFDAVVSDFAAGFMDLDLLLPE
                     IAAVLRPGGFVALAWTSPILDCMTGAGEPPLLAFKYSYFDRTPWVDGGRDATFEFKRT
                     YGDWVRAFARAGLMLEDLVEPQAPRGAPHMEWGNFRWERTSMVPGTCIWKARKPDKPT
                     RRRA"
     gene            2058..2420
                     /locus_tag="VM841_15575"
     CDS             2058..2420
                     /locus_tag="VM841_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91641.1"
                     /translation="MSDTPHTDRLMAELRGKLDSISNSAEQSRAFKAADDAYATWLAR
                     HADTGYILHRKTAATKTATLHQAACERIAPSEEAELFKGPRVGCPTKPVALAIALSKK
                     WKVRDDCPSCVAAETPEM"
     gene            complement(2622..2695)
                     /locus_tag="VM841_15580"
     tRNA            complement(2622..2695)
                     /locus_tag="VM841_15580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2659..2661),aa:Ile,seq:gat)
     gene            complement(2755..3135)
                     /locus_tag="VM841_15585"
     CDS             complement(2755..3135)
                     /locus_tag="VM841_15585"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HVL91642.1"
                     /translation="MPATAGPVQYSRQWRKARLVIRKVDPWSALKFSVLFYFCLLLIM
                     LLGSAIVFALLKAFGVIGRIEELLGTFFADVTISGGAIFRWVFLFGLLGTIVSTVVTV
                     FLAFLYNLIADVVGGVEISVSQRD"
     gene            complement(3301..7020)
                     /gene="gyrA"
                     /locus_tag="VM841_15590"
     CDS             complement(3301..7020)
                     /gene="gyrA"
                     /locus_tag="VM841_15590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVL91643.1"
                     /translation="MTDTPLEPRIEAIDIEEEVKKSYLDYAMSVIVGRALPDVRDGLK
                     PVHRRILHAMNEMGVRPGTAFKKSARVVGETMGKFHPHGDQAIYDALARMAQDFAMRA
                     PLIQGHGNFGSVDGDPPAAQRYCVTGDTLVRTVSGTVRIRDLVPDAKPNSDTPIVEKL
                     INRNGDPVRASMFFHSGTHPILRLKTREGFTLEGTGNHPVLCLVPVAGVPMFQWKELH
                     EIVAGDRVAMLRREPGESEVLSQHDWHLAILAGAMVSEGFASDTRAGFNNLDKDFFSQ
                     VVAAFDEIVGGSRYVSSRVIKSGNLLYELDVQKMDHFAASPLGELMGDRSASKHVPSF
                     VWSAGPAFKAAFLRALFEGDGSSSVGNRSSIGISYTTRSRLLAQEVQQLLLEFGIISR
                     LTCTVARGEHRVYISNRRDAWLFATRVGFLEHKQHRLLAQFVEIPEQSHSLSSDHFPY
                     IAAYIRGEKSGRWVDREWLAKHNIDRIERWERDADEIWSHITNAEVRSVVEPIVESGY
                     YFAEVAEISSAGLAEVYSVRVDSEDHAFITNGFVSHNTECRLAPLAMEILRDINEETV
                     DFVPNYDGEESEPSVLPARFPNLLVNGSTGIAVGMATNIPPHNLGEVIDATIHLIDNP
                     DATVEDLMKFVKGPDFPTGGIIMGRAGARQALETGRGSVKVRGKAEIEEGKNGRQRII
                     ITELPYMVSGDRVLAKIAEMVEKKVITGIATTRDSLKNESNRHGLRLVVELKRDAIPQ
                     VVLNNLYKHTQLQDSFGVNMLALVDGVPRTLSLVDVLTAYVGHQVEVVTRRTRYRLRK
                     AEERAHILEGLLVALNHLDDVIALIRASDDVEAARTGLMERFDLSEIQANAILDMQLR
                     RLAALERQKIIDEHTELQAQIADYKDILGKPERVRTIIKDEMSEIRAKFANDRRTAVQ
                     ADEGDMDLEDLIPDEECVITITRSGYIKRVNLSVYRTQGRGGKGVRGAKPKEGDIVEH
                     LLTTTAHAYVLFFSSLGKVYRIKAHEIPEKDRTARGISIRNLLPLSGEDAIAAVIDTR
                     DYETHKYLVIATAKGIVKRTEFSAYDSSRRDGIIALKLREGDQVVQVRATSGEDELVM
                     ATRKGQSIVFPESEVRAMGRTASGVIGMKIKAEDTVMAFEVVDPNGEFLIVTDNGYGK
                     RTLMTNYPRQHRGGSGVRTVQLTARKGFVAGAMVVRPGHELFLISSNGQVIRIQAKEI
                     RRTGRDAQGVRVMRLGSGETVAAMAPVIADEEEGP"
     gene            complement(7029..8939)
                     /gene="gyrB"
                     /locus_tag="VM841_15595"
     CDS             complement(7029..8939)
                     /gene="gyrB"
                     /locus_tag="VM841_15595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HVL91644.1"
                     /translation="MARASSEYGSSDIQVLEGLEAVRRRPGMYIGGTNQRGLHHLVYE
                     VVDNAIDEALAGRCDLIEVVLRGDGGVSVTDNGAGIPVQPIPGAKDKRPAVEVVLTVL
                     HAGGKFGGKGYAVSGGLHGVGVSVVNALSEKCEVEVHRDGAIWETAFARGKSTRKLEK
                     TGTAKDTGTTVRFWPDPQLFPEIEFKFDILAQRLRELSYLTKGVTITLRDERGETVEE
                     ERFVASGGIADMVKALNAGREGLNKIIHFEAGEEEREVEIAIQWNAGFTDSIHTFANT
                     INTAEGGMHEEGFKKSLTNIVNKYARAKGLLKEKEENLQGEDIREGIVAIISVKLREP
                     QFEGQTKTKLGNTEIRSFVEKVMNDKFGDWFEEHPADAKRIVAKALSAQRARLAAKKA
                     RDIARKSVFDAGGLPGKLADCSSSDPNNTELFIVEGDSAGGSSKAARNREFQAILPIR
                     GKILNVEKARLHKILENAEITALIQAIGTGIGDEADLSKRRYGKIVMMADADVDGAHI
                     RTLLLTFIFRHMKIMIEGGHVYIAQPPLYRVTVDAKTKFYAFNDKERDEIVKQYPNKK
                     INVQRFKGLGEMNADELWDTTLNPETRTLLQVTMEDAALADQIFTILMGEDVESRRAF
                     IQKNAKDAFVDV"
     gene            complement(9207..9530)
                     /locus_tag="VM841_15600"
     CDS             complement(9207..9530)
                     /locus_tag="VM841_15600"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HVL91645.1"
                     /translation="MSERDPVRIGSVLGQMIHREGWRPRLALGRLRASWSEIVGPQIG
                     ANSYPGKLSDGVLLIFAESGAWATELALLAGRLAVKADAFLGGGLVREVKVVARGGGR
                     ASKRG"
     gene            complement(9527..10663)
                     /locus_tag="VM841_15605"
     CDS             complement(9527..10663)
                     /locus_tag="VM841_15605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HVL91646.1"
                     /translation="MRLAAISLEHFRSWESLALTFEDGVTVIRGPNGSGKTTVLEAAW
                     YAAALGSHRTSSDEVLVRRGEVSGFVRADVARAVVGGGERIEKIELEVRTGGRARTKL
                     GGANVPRRRDVLGIMRAAIFAPERVSVVRGDPGDRRRFADDLLVQLHPRYHAVIKEYE
                     RALRQRNALLRDVAAGRARPQGIEAWDEALAKPGGELAAGRAKAVAALAPFAGKAYAA
                     VGEGEALVVSYTPRTAPAGFEPGATEPASAEWTDAIRVALAERRTDEMVRGTTLAGPH
                     RDDLTITIGGLPARTHASQGEAWMAAVGIVLGSHDAIGAHLGEAPVLLLDDAFNPLDP
                     ARRQRLAGALPDGTQILISAADPAEVPAGLEARVIDVDRYRSPR"
     gene            complement(10674..11759)
                     /gene="dnaN"
                     /locus_tag="VM841_15610"
     CDS             complement(10674..11759)
                     /gene="dnaN"
                     /locus_tag="VM841_15610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVL91647.1"
                     /translation="MKIKIERDVFADAVAWVLRSVGSRATLPVLGGVLIEADGDTITF
                     TGTDLEVSSEAKVQAAVAREGKVLLPGRVLGDIARSLPEGTVELTADQSMGKIVCGAA
                     EFTLRTLPVEDFPQIALPDADAATIDGKLFASAVAQVTKAASHDEARPILTGVLLEAG
                     AKTLTLVATDSYRLAVREIDWTAPGIEGKRVVPARALVEAVKAAEGESEIKITLGESQ
                     VSVEAGGRTLVSRLIEGEFPNWRQLIPTDIPSHLTIARDAFADSVRRVGILAQQGAPV
                     RLELNADGVKLLAGTQDLGEANEAAGGTFEGEAITVAFNPVYLNDGIAAAGGAEIKLS
                     LRDGLKPALVTSTEQDGFTYLLMPVRI"
     gene            complement(11921..>12986)
                     /gene="dnaA"
                     /locus_tag="VM841_15615"
     CDS             complement(11921..>12986)
                     /gene="dnaA"
                     /locus_tag="VM841_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVL91648.1"
                     /translation="PKEQPDKSVLNPKYVFDTFVIGPSNRFAHAAALAVAEQPARSYN
                     PLFIYAPPGLGKTHLLHAIGHHVRSLYPTAAVRYVSSEQFMNEFILGVREEKMPAFRR
                     RYREADVLLVDDIQFLARGEQTQEEFFHTFNALHNDGRQIVITSDRPPNQIAPLEDRL
                     RTRFEWGLITDMQAPDLETRMAILQKKANAEGFIVPDDVLEFLAGRVQNNIRELEGRL
                     IRIVSYASLSNQPVTMDLAQEVLRPLLPPDSANDIAVDAIIAETLSYFGLTRDELLGP
                     SRSRHLVHARQIAMYLCRELTPLSLPKIGEAFGGRDHTTVMHADSKIRKWLGEKRPVY
                     SQVQELTARIRQRGATGLSA"
BASE COUNT         2037 a         4404 c         4275 g         2270 t
ORIGIN      
        1 gtcctcttcg gggcgatgac gccgggccgc gagcttgatc acgccgttga cgactgcgga
       61 ggtaagcgac agcgaggcca tgccgcggag cgcggctttg cgttcgcgcg ggccggtgaa
      121 ggcgagtgcg gccgcgatgc ccatccagag ccggccgccg ttcgcagagc gagaaagcgc
      181 gggcagcagg cgatccaggg acgaggcggg catggccgcg acccgcgcga tgcccgcctc
      241 gtcgagccgg tgtgcccact cggggaggaa cggccggtcg tggcgggcga gtatcagccg
      301 aatgatcgca tcgcgcatcg catcccgaat gttccttgct ctgacggatg acaaaccttg
      361 ctcgcgggta cagtggagtc gatgcctaga cgatctccgg agcagcgcat gatgaaatgg
      421 atgaccgcgg gcgcctacac gatgcgccac tggcggggca tcctgctcag catctggctg
      481 ggaaccgtgg cgacattcgg cctgctggcc tggtggggct tcggcaatcg cgacagcggt
      541 ttcggcgctt tcgtgttcac ggtcttcacg ctgctcgtca tcgcgatggt gtttcgatat
      601 ccgcgacggc tccttcggtc gtggcgtacg tcacggcggg ggcctcccgc tgtgggacga
      661 cccggcggcg tgatcgatgc cgaggccgat gtccgcgacc gcccgcggcg cgagccgcca
      721 ccgctcggac cgcggcgtcc ttgaccgcaa cgggccgcag cgcggcttct ccggacgcgc
      781 ggaggcccgc cggccgttcc cgacatcgag agcagcaggc gggcctcggc gagagtcgtc
      841 cggcctaggc ggagcctccc ggcgtcgcct cttcccagcc ggtctcttcg ccgttccgac
      901 gcttcttctt caagaagaag aacacggcgc ccacggccgc aagcaacacg gcgaagattc
      961 ccttcttcga gcgcttcttc tcttcctcgg ccatcagccg ccccctcctc tcgccgcgcc
     1021 ggagcgcgct ggtttttttt ggatgatatc cccgactttg ccgccgcaac aagagcgaca
     1081 gttgcaagcg atcctacgcg cggcgcctcg tcggcttgtc cggcttccgc gctttccaga
     1141 tgcatgtgcc gggcaccatg ctggttcgct cccaccggaa atttccccac tccatgtggg
     1201 gggcgccgcg cggcgcctgc ggctcgacga gatcttcgag catcaatccc gcgcgagcga
     1261 aggcacgaac ccagtcgccg taggtgcgct tgaactcgaa ggtcgcgtcc cgcccgccgt
     1321 ccacccacgg tgtgcggtcg aagtaggagt acttgaatgc gagcagcggc ggctcgcccg
     1381 cgccggtcat gcaatcaaga atcggcgagg tccacgcaag cgccacgaag ccgcccggcc
     1441 gcagaaccgc cgcgatctcg ggcagcagca gatccagatc catgaatccg gccgcgaaat
     1501 cgctgacgac cgcgtcgaag gtgccttcgc cgaatcgcgc gcgcagcttt tcggcgttgc
     1561 cggtgatcag ccgcatcttc acgccggcgg ccttgacgtt ggcgcgtccg tgaaggatct
     1621 gctcgcccga caggtcgatc cccgtcatgc gcgcgccccg tcgcgcgagc tcgatgccga
     1681 actgcgcggc gccgcatccg atctcgagga cttcgaggcc ggcgacgtcg ccgatcacgc
     1741 ccagatgctc gtcgtcgaac ttgttcggcc cccagtgaat acgtccggca tacatgtccc
     1801 ggccgccgac ggcttgctga tagcgggccg cccggtcgtc ccagccgcgc cggttcgcgg
     1861 cgatacggtc ggcgcgcacc ctgcgaacac gatccgactg cgtggaccgg gcccggatcc
     1921 catccgagtc gctcatggcg gccatcatag gtcgagtccg aaatctctcg gcgcccaccg
     1981 gccgtttcga acgcgacccg cgagactcgg ataaagttcc acccgatgcc cagggtgggc
     2041 cggaagaggt gacgctggtg tcggacacgc cccatacgga ccggctcatg gccgagcttc
     2101 gcggaaagct cgactccatc tccaactccg ccgagcaatc acgcgccttc aaagccgccg
     2161 acgacgccta cgccacctgg ctggcccgtc atgccgacac cggctacatc cttcaccgca
     2221 agaccgcggc caccaagacc gccacgctcc accaagccgc atgcgagcgc atcgccccct
     2281 ccgaggaggc cgagctgttc aaaggccccc gcgtcggctg ccccaccaaa cccgtcgccc
     2341 tcgccatcgc cctgagcaaa aagtggaagg tccgggacga ctgcccatcc tgcgtcgccg
     2401 ccgaaacccc cgagatgtag cctcgcaaca agggccaatc gctcgagtcc ccggacgagt
     2461 cgctgctgaa cggtcccgcc tgtccgagtg aaagcgcggc gttatcgacc cctcgtaaga
     2521 atcggccaac gctccgaccg cggacgcgat cttttcgcgg gggtttgggg gcagagggcg
     2581 ccgagcgaag cgaggggccc ggagccccta atacatccaa gtgggcctgg ctggactcga
     2641 accagcgacc tcagctttat cagagctgcg ctctagccac ctgagctaca ggcccgtgcg
     2701 aatgcagaag ggtaccagga tccgcgctcg gcgtcgcagg ggtgacgggc gaggctagtc
     2761 gcgctggctg acgctgatct cgacgccccc aacgacgtcg gcgatcaggt tgtacaggaa
     2821 ggccaggaac acggtcacga cggtggacac aatggtcccg agcaggccga acaggaacac
     2881 ccagcggaag atcgctccgc ccgagatggt cacgtccgcg aagaacgtgc cgagcagctc
     2941 ttcgatccgg ccgatcacgc cgaaggcctt gagcagagcg aacacgatgg cgctgccgag
     3001 cagcatgatc agcagcaggc agaagtagaa cagcaccgag aacttcaagg ccgaccaggg
     3061 gtcgaccttg cggatgacca ggcgggcctt acgccactgc cgcgaatact gcacgggccc
     3121 ggccgttgcg ggcacgacgt cccggctggc cgtctcgcta cgtgcggtag gcgcggtggc
     3181 gcccgtctcg tgcttgtcga tcgtgtcccc tcggaatgct tgcggcccgt gcatgggcct
     3241 tgatgttccc cggctcgctt gttcttacac ggccgagccg ggatccgcca atagcccgcg
     3301 ctaggggccc tcctcctcgt cagcgatcac cggcgccatc gcggcaaccg tttcgcccga
     3361 ccccaggcgc atcactcgca cgccctgggc gtcacgcccg gtgcggcgaa tctccttggc
     3421 ttggatccgg atcacctggc cgttggacga gatcaggaac agctcgtggc ccggcctcac
     3481 gaccatcgcg ccggcgacga aacccttgcg cgcggtcagc tggaccgtgc gcactcccga
     3541 gccgccgcgg tgctggcgcg gatagttcgt catcagcgtc cgcttgccgt agccgttgtc
     3601 ggtcacgatc aggaactcgc cgttgggatc gacgacctcg aaggccatga ccgtgtcttc
     3661 ggccttgatc ttcatgccga tcacgccact ggcggtgcgg cccatggcgc ggacctcaga
     3721 ttcggggaag acgatcgact ggcccttgcg ggtggccatg accagctcgt cttcgcccga
     3781 cgtggcacgc acctgcacga cctgatcgcc ttcgcgcagc ttgagcgcga tgatgccgtc
     3841 tcgccgcgac gaatcgtagg ccgagaactc ggtgcgcttg acgatgccct tggcggtggc
     3901 gatcaccaga tatttgtggg tctcgtagtc gcgggtgtcg ataacggctg cgatcgcgtc
     3961 ctcgcccgac agcggcagca ggttgcggat cgagatcccg cgcgcggtgc gatccttttc
     4021 ggggatctcg tgcgccttga tccggtagac cttgcccagc gacgagaaga acagcacgta
     4081 ggcgtgcgcc gtcgtggtga gcagatgctc gacgatgtcg ccttccttcg gcttggcgcc
     4141 gcgcacgccc ttgccgccac ggccttgcgt ccggtacacg ctcaggttga cgcgcttgat
     4201 gtagccggag cgtgtgatgg tgatcacgca ttcctcgtcg gggatcaggt cctcgaggtc
     4261 catgtcgccc tcgtcggcct ggaccgcggt gcggcgatca ttggcgaact tggcgcggat
     4321 ctcgctcatc tcatccttga tgatcgtgcg gacacgctcg ggcttgccga ggatgtcctt
     4381 gtagtcggcg atctgcgcct gcagctcggt gtgctcgtcg atgatcttct gccgttccag
     4441 cgcggcgagc cggcgaagct gcatgtccag gatcgcgttc gcctggatct cgctcaggtc
     4501 gaagcgttcc atcaggccgg tgcgcgcggc ctcgacgtcg tccgaggcgc gaatgagcgc
     4561 gatcacgtcg tcgaggtggt tgagcgcgac cagcagacct tccaagatgt gcgcgcgctc
     4621 ttcggccttg cgcaggcggt agcgggtgcg ccgcgtgacg acctcgacct gatggccgac
     4681 gtaggcggtg agcacgtcca ccagcgacag cgtgcgcggc acgccgtcca ccagcgcgag
     4741 catgttcacg ccgaacgagt cctgcagctg cgtgtgcttg tagaggttgt tgagcacgac
     4801 ctgggggatc gcgtcgcgct tgagctcgac gaccagtcgc aggccgtgac ggttcgactc
     4861 gttcttgagc gagtcgcgcg tggtcgcgat gccggtgatg actttcttct cgaccatctc
     4921 ggcgatcttg gccagcaccc ggtcgcccga gaccatgtag ggaagctcgg tgatgatgat
     4981 gcgctgacgg ccgttcttgc cttcttcgat ctctgctttg ccgcgcacct tgaccgagcc
     5041 gcggccggtc tcgagcgcct gccgcgcgcc ggcgcggccc atgatgatgc cgcccgtcgg
     5101 gaagtcgggg cctttgacga acttcatcag gtcttcgacg gtcgcgtcgg gattgtcgat
     5161 cagatggatc gtcgcgtcga tgacctcgcc caggttgtgc gggggtatgt tggtggccat
     5221 gccgaccgca atgccggtcg agccgttgac gagcaggttc gggaagcgcg ccggcaggac
     5281 cgacggctcg gattcctcgc cgtcgtagtt gggcacgaag tcaacggtct cttcgttgat
     5341 gtcgcgcagg atctccatcg cgagcggcgc gagccggcac tcggtgttgt ggctgacgaa
     5401 gccgttcgtg atgaacgcgt ggtcttcgga gtccactcgg acggagtaga cctccgcaag
     5461 ccctgcggac gagatctcgg cgacctccgc gaagtagtaa ccgctctcga cgatcggctc
     5521 gacgaccgag cgcacttcgg cgttggtgat gtgcgaccag atctcgtctg cgtcgcgctc
     5581 ccaccgctcg atgcggtcga tgttgtgctt ggcgagccat tcgcgatcga cccatcggcc
     5641 ggacttctcg ccacggatgt aggcggcgat gtacgggaag tggtccgagc tgagcgaatg
     5701 gctctgctcg gggatctcga cgaactgcgc aagcaggcgg tgctgcttgt gctccaagaa
     5761 tccaacgcgt gtcgcgaaca accacgcatc acgccggttc gagatgtaga cgcgatgctc
     5821 gccgcgcgcg accgtgcagg tcagccggga aatgatcccg aactcgagca gaagctgctg
     5881 gacttcctgt gccagcagcc ggctgcgcgt cgtataggag atcccgatcg acgaccggtt
     5941 accaaccgac gacgagccgt ccccttcgaa cagcgcacgg aggaacgcag ccttgaacgc
     6001 gggccctgcg ctccacacaa aggacggcac gtgtttcgag gcgctccggt cacccatcag
     6061 ctcgccgagc ggacttgccg cgaagtgatc catcttctgc acgtcgagtt catacagaag
     6121 gttgccggac ttgatcaccc gcgacgacac gtagcgcgaa ccgcccacga tctcgtcgaa
     6181 ggcggccacg acctgactga agaagtcctt gtcgaggtta ttgaacccgg cgcgcgtgtc
     6241 ggacgcgaag ccttccgaga ccatcgcgcc cgccaagatc gccagatgcc agtcgtgttg
     6301 cgaaagcacc tcggactcgc ccggctcgcg gcgaagcatc gccacgcgat cgccggcgac
     6361 gatctcgtgc agttccttcc actggaacat cggaacgccg gcgaccggca ccagacacag
     6421 caccggatgg ttgccggtgc cttccagtgt gaacccttct cgcgtcttca accgcaggat
     6481 cggatgcgta cccgaatgga agaacatcga ggcgcgcacc ggatccccat tccggtttat
     6541 cagcttctcg acgatcggcg tgtcgctgtt cggctttgcg tcgggaacca gatcgcggat
     6601 gcgcaccgtt ccgctgaccg tgcggaccag cgtgtcgccg gtgacgcaat aacgctgtgc
     6661 ggccggcggg tcgccgtcca cgctgccgaa gttgccgtga ccttggatca gcggcgcgcg
     6721 catcgcgaag tcctgcgcca tgcgtgccag cgcgtcatag atcgcttgat cgccgtgagg
     6781 atgaaatttg cccattgtct cgccgacgac cctggcgctc ttcttgaacg ccgttccggg
     6841 gcgcacgccc atctcgttca tggcgtgcag gatccggcgg tgcaccggct tgagaccgtc
     6901 gcgcacgtcg ggcagcgcgc ggccgacgat gaccgacatc gcgtagtcga ggtaggactt
     6961 ctttacctct tcctcgatgt cgatcgcttc gatgcgtggt tccagcggag tatcggtcat
     7021 cgttttcctt acacgtccac gaacgcgtct ttggcgttct tctggatgaa tgcgcggcgc
     7081 gactcgacgt cttcgcccat caagatggtg aagatctgat cggcgagcgc ggcgtcttcc
     7141 atcgtgacct gcagcagcgt gcgcgtctcg gggttgagcg tggtgtccca cagctcatcg
     7201 gcgttcatct cacccagacc tttgaaacgc tgcacgttga tcttcttgtt cgggtactgc
     7261 ttcacgatct cgtcgcgctc tttgtcgttg aacgcgtaga acttggtctt ggcgtccacg
     7321 gtgacccggt acagcggcgg ctgcgcgatg tagacgtgac cgccctcgat catgatcttc
     7381 atatggcgga agatgaacgt cagcagcagc gtgcggatgt gcgcgccgtc cacgtcggcg
     7441 tcggccatca tcacgatctt gccgtagcgc cgcttcgaca ggtcggcctc gtcgccgata
     7501 ccggtgccga tcgcctggat cagcgcggtg atctcggcgt tctccaggat cttgtgcagc
     7561 cgcgccttct cgacgttgag gatctttccg cggatcggaa ggatcgcctg aaactcgcgg
     7621 ttgcgcgccg ctttggacga gccaccggcc gagtcaccct cgacgatgaa cagctcggtg
     7681 ttgttcgggt cgctggagga gcagtcggcg agctttccgg gaagaccgcc ggcgtcgaag
     7741 acgctcttgc gcgcgatgtc gcgggccttc ttggcggcca gccgcgcccg ctgcgcgctg
     7801 agcgccttgg cgacgatgcg cttggcatcg gccgggtgct cttcgaacca gtcgccgaac
     7861 ttgtcgttca tgaccttctc gacgaacgag cggatctcgg tgttgcccag cttggtcttg
     7921 gtctggccct cgaactgcgg ctcgcgcagc ttgaccgaga tgatcgccac gatgccttcg
     7981 cggatgtcct cgccctggag gttctcttcc ttctccttca acagaccctt cgcgcgggcg
     8041 tacttgttga cgatgttggt cagggacttc ttgaagccct cttcgtgcat gccgccttcg
     8101 gcggtgttga tggtgttggc gaaggtatgg atcgagtcgg tgaagcccgc gttccactgg
     8161 atcgcgatct cgacctcgcg ctcttcctcg ccggcctcga agtggatgat cttgttcagg
     8221 ccctcgcgtc cggcgttgag cgccttgacc atgtcggcga tgccgcccga ggcgacgaac
     8281 ctctcttcct cgaccgtctc gccccgctcg tcgcgcagcg tgatcgtgac gcccttggtc
     8341 aggtagctga gctcgcggag ccgctgggcc aagatgtcga acttgaactc gatctcgggg
     8401 aacagctgcg ggtcgggcca gaaccggacc gtggtcccgg tgtccttggc cgtgccggtc
     8461 ttctcgagct tgcgagtgct cttgccgcgc gcgaaggcgg tctcccaaat cgccccgtcg
     8521 cggtgcacct cgacctcgca cttctccgac agcgcgttga cgaccgacac gccgacgccg
     8581 tgcagaccac ccgagacggc atagcccttg ccgccgaact tgccgccggc gtgcagcacg
     8641 gtgagcacga cctcgaccgc gggccgcttg tccttggcgc cggggatcgg ctgcacgggg
     8701 atcccggcgc cgttgtcggt gacgctcacg ccgccgtcgc cgcgcaggac gacctcgatc
     8761 aggtcgcagc ggccggcgag cgcctcgtcg atcgcgttgt cgacgacctc gtagaccagg
     8821 tggtggaggc ctcgctggtt ggtgcctccg atgtacatgc cgggccggcg gcgaaccgcc
     8881 tccagccctt cgagtacttg gatgtcgctc gagccgtatt cggacgaggc gcgggccact
     8941 cgatcactcc ttcatccccg gctgcggccc ggccccgcag ggcccgccgc gcgcgaggca
     9001 agcatgggta tgacagcaaa aacgccccca ccggcaggtc ccacgacccg atccggacac
     9061 ccggggcgag ctccggcggg aaggatgccg gaggcacacg tttattatag cgcccgaggg
     9121 cgggaaaagc cgcttcggcg gcctcgattc cggggtctgc gaacactttc cggatcgatc
     9181 cgaaaaaccc gttctgacct gcggttttat ccccgcttcg aggcccgtcc gccgccgcgg
     9241 gcgaccacct tgacctcccg caccagaccc ccgcccaaaa aggcatccgc cttgaccgcc
     9301 aaacggcccg cgagcaaggc aagctctgtg gcccaggcgc ccgattcggc gaagatgagc
     9361 aggaccccgt ccgagagctt tccggggtag gagttggccc cgatctgagg cccgacgatc
     9421 tcgctccagc tcgcccgcag ccggccgagc gcgaggcggg gacgccatcc ctcgcgatgg
     9481 atcatctggc cgagcaccga tccgatgcgc accgggtcgc gttcgctcat cgcgggctcc
     9541 gataccggtc cacgtcgatc acgcgcgctt cgagcccggc cggcacctcg gccggatccg
     9601 cggcgctgat caggatctgg gtgccgtcgg gcagcgcgcc ggcgagccgt tgccgccgcg
     9661 ccgggtcgag ggggttgaac gcatcgtcga gcagcagcac cggcgcctca cccaggtgcg
     9721 cgccgatcgc gtcgtgggat cccaggacga tgcccaccgc cgccatccac gcctcgcctt
     9781 gcgaggcatg ggtgcgagcg ggaaggcccc cgatcgtgat ggtcaggtcg tcgcggtgcg
     9841 gcccggcgag cgtggtgccg cgaaccatct cgtccgtgcg tcgctcggcg agcgcgacac
     9901 ggatcgcgtc ggtccattcc gcggaagcgg gctcggtcgc ccccggctcg aatccggccg
     9961 gcgccgtccg cggcgtgtag gagaccacga gcgcctcgcc ctcgccgacg gcggcgtagg
    10021 ccttccccgc gaacggcgcg agcgcggcga cggccttcgc gcgtcccgcc gcgagctcgc
    10081 ctcccggctt ggcgagcgcc tcatcccagg cctcgattcc ctgcggccgg gcccggccgg
    10141 cggccacgtc gcgcagcagg gcgttacgtt gccgcagcgc ccgctcgtac tccttgatca
    10201 ccgcgtggta gcgcggatgg agctgcacga gcagatcgtc ggcgaagcgg cgccggtcgc
    10261 ccggatcacc gcgcacgacc gagacacgct cgggcgcgaa aatggcggcg cgcatgatgc
    10321 cgagcacgtc gcgccggcgc ggcacgttgg cgccgcccag cttcgtgcgc gcacgtccgc
    10381 ccgtgcgtac ctcgagctcg atcttctcga tccgctcgcc gcccccgacc accgcccggg
    10441 ccacgtcggc ccgcacgaac ccggagacct cgccccggcg cacgagcacc tcgtcgctgc
    10501 tggtgcggtg cgagccgagg gccgcggcat accaggcggc ctccagaaca gtggtctttc
    10561 cggacccgtt cggccctcga atcaccgtga cgccgtcctc gaaggtcagc gccagggact
    10621 cccacgaacg gaagtgctcc agcgagatcg cggcgagccg cacacgcgcc ccgctagatg
    10681 cgcacgggca tcagcaagta cgtgaacccg tcctgctccg tcgaggtcac cagcgccggc
    10741 ttgagcccgt cccgcagcga cagcttgatc tcggcgccgc ccgcggccgc gatcccgtcg
    10801 ttgaggtaga ccgggttgaa cgcgaccgtg atcgcctcgc cttcgaacgt tccgcccgcg
    10861 gcctcgttgg cctcccccag gtcctgggtt ccggccagga gcttcacgcc gtcggcgttg
    10921 agctcgagcc gcaccggcgc gccctgctgg gcgaggatgc cgacgcggcg gaccgagtcg
    10981 gcgaacgcgt cccgcgcgat ggtcaggtgg ctcgggatgt cggtcgggat gagctggcgc
    11041 cagttcggga actcgccttc gatcagccgc gacaccagcg tccgtccgcc ggcctcgacc
    11101 gagacctgcg actcgccgag cgtgatcttg atctcactct cgccctcggc cgccttgacc
    11161 gcctcgacga gcgcgcgggc cggcacgacc cgcttgcctt cgatgcccgg cgcggtccag
    11221 tcgatctcgc gaaccgcgag gcggtaggaa tcggtggcga ccagcgtgag cgtcttggcg
    11281 ccggcctcga gcagcacgcc ggtcaggatc ggacgggcct cgtcgtgtga tgcggccttg
    11341 gtcacctgcg cgaccgcgga cgcgaagagc ttgccgtcga tcgtggcggc gtcggcgtcg
    11401 ggcaacgcga tctgggggaa gtcctcgacc ggcagcgtgc gcagcgtgaa ctcggcggcg
    11461 ccgcaaacga tcttgcccat cgactgatcg gcggtcagct cgaccgttcc ttccggcagc
    11521 gaccgcgcga tgtcgccgag cacgcgcccg ggcagcagga ccttgccttc ccgtgcgacc
    11581 gcggcctgga ccttggcttc gctcgagacc tcgaggtcgg ttccggtgaa cgtgatcgtg
    11641 tcgccgtcgg cctcgatcag cacgccgccc agaaccggca gcgtggcgcg cgatccgacg
    11701 gaacgcagca cccaggcgac cgcatccgcg aacacgtccc gctcgatctt gatcttcatc
    11761 gaagagccct cctccacaac cttagcgctg cgctacgaca gtgatctttt tcttcacaaa
    11821 aactagaagc aacagtaggg gctggggatt cggtggatga acccccgttc gtgcaggccg
    11881 aagcacacga aaccgttcac attcgggtgt gggaaaccgg tcatgccgaa agccccgtgg
    11941 cgccccgctg gcggattctg gccgtcagct cctgaacctg tgagtacacg ggacgctttt
    12001 ccccaagcca cttgcggatc ttggagtcgg cgtgcatgac cgtggtgtgg tcgcgtccgc
    12061 cgaaggcttc gccgatcttg ggaagggaca gcggggtgag ctcgcggcac aagtacatcg
    12121 cgatctgccg ggcgtgcacc agatgccgcg accgcgacgg gcccagcagc tcgtcgcggg
    12181 tgagaccgaa gtaggacagc gtctcggcga tgatggcgtc cacggcgatg tcgttggccg
    12241 agtcgggcgg cagcagcggc cgcaacacct cttgggcgag atccatggtg accggctggt
    12301 tcgacagcga cgcgtaggag acgatgcgga tcaagcgtcc ctccagttcg cggatgttgt
    12361 tctgcacgcg gccggcaagg aactcgagca cgtcgtcagg aacgatgaat ccctcggcgt
    12421 tggccttctt ctgaaggatg gccatgcggg tctcgaggtc gggcgcctgc atgtcggtga
    12481 tcaggcccca ttcgaaccgg gtgcgcagcc ggtcctcgag cggcgcgatc tggttcgggg
    12541 gacggtctga ggtgatcacg atctgacggc cgtcgttgtg cagcgcgttg aaggtgtgga
    12601 agaactcctc ttgggtctgc tcgccgcgag cgaggaactg gatgtcatcg acgagcagca
    12661 cgtcggcttc gcggtagcgg cgccgaaagg cgggcatctt ctcttcgcgc acgccgagga
    12721 tgaactcgtt catgaactgc tcgctggaga cgtagcgcac cgcggcggtc ggatacaggc
    12781 tgcggacgtg gtggccgatc gcgtgcagaa gatgcgtctt ccccagcccg ggtggcgcgt
    12841 agatgaacag cgggttgtag gagcgggcgg gttgctcggc gaccgcgagg gcggccgcgt
    12901 gggcgaaccg gttcgacggg ccgatcacga acgtgtcgaa cacgtacttg gggttgagca
    12961 cgctcttgtc cggctgctcc ttgggc
//
LOCUS       DATLFO010000380         3488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_128537, whole genome shotgun sequence.
ACCESSION   DATLFO010000380 DATLFO010000000
VERSION     DATLFO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128537"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /gene="rplR"
                     /locus_tag="VM841_15620"
     CDS             <1..240
                     /gene="rplR"
                     /locus_tag="VM841_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVL91649.1"
                     /translation="HIYAQVIDDTAGATLAAASSMKLSVDGDKKSAARKVGEMVAEAA
                     KQKGVSKVVFDRGGNRYHGRVKEVAEGARAAGLEL"
     gene            250..939
                     /gene="rpsE"
                     /locus_tag="VM841_15625"
     CDS             250..939
                     /gene="rpsE"
                     /locus_tag="VM841_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HVL91650.1"
                     /translation="MVERRRSGKDEDAGRPALEERVVSINRVAKVVKGGRRFSFTALV
                     VVGDKDGKVGIGYGKAKEVPTAIQKGVDIARRSMFTVPMVGQTIPHEVMGELGAGRVL
                     LKPAAPGTGVIAGGPVRAVMECAGIHDVLSKSLGSGNAINMIHATVEGLKRLRRPETV
                     AATRGLDIRRVAPRRMLEAAGVSTDAIDRLERQDERAAAAEPAPAAGTSAPEAPTAEA
                     PATEAAAGGES"
     gene            939..1124
                     /gene="rpmD"
                     /locus_tag="VM841_15630"
     CDS             939..1124
                     /gene="rpmD"
                     /locus_tag="VM841_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HVL91651.1"
                     /translation="MAKLKVTQVKSVIGTTQDMRATVRSLGLHRIRDEAIVDDRPEIR
                     GMINKVKHLVKVEEVRS"
     gene            1121..1558
                     /gene="rplO"
                     /locus_tag="VM841_15635"
     CDS             1121..1558
                     /gene="rplO"
                     /locus_tag="VM841_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVL91652.1"
                     /translation="MKLHHLRPAPGAKRPKKRYGRGEASGHGKTAGRGTKGSRARGEV
                     HPWFEGGQMPLHRRIPKLGGFTPRTRTEYREVNVDRLNEFGSGATVSPEDLIAKGLLR
                     KGKQPVKVLGRGELTVALTVKANAFTAGARQKIESAGGRAEVL"
     gene            1568..2857
                     /gene="secY"
                     /locus_tag="VM841_15640"
     CDS             1568..2857
                     /gene="secY"
                     /locus_tag="VM841_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVL91653.1"
                     /translation="MFSAFRNAFKVPDLRNKILFTIFVIALYRLGSHIPVPGIDFAKV
                     EELKAQASQGGIYSLINLFSGGALTQFAIFALGIMPYITSSIIMQLLTVVIPRLEAYQ
                     KEGEAGQKKITQYTRYMTVVLALLQSLGIVVTANSGRLFGEGIRDLFGPNLTSGRTFI
                     VVLTLTAGTAMIMWLGELITQRGIGNGMSILIFASIISRLPAESTALWTNAGKLKALV
                     FLGVGILIIVAVVLVEQAQRRIPVQYAKRVVGRKMYGGTSTYIPLKVNQAGVIPIIFA
                     SSLLYLPVLASTIVQSAGFQRFVNRFLENGSHPAYILIYFLLIIFFTYFYTSITFNPV
                     DVADNMKKYGGFIPGIRPGRPTAEYLDHILTRITLPGSFFLGIIAVMPFILQGIWNIT
                     SFPFAGTSILIIVGVALETNKQIESQLMMRHYEGFLK"
     gene            2860..>3488
                     /locus_tag="VM841_15645"
     CDS             2860..>3488
                     /locus_tag="VM841_15645"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HVL91654.1"
                     /translation="MRIVMLGPPGAGKGTQAAMLADKFGVPHVSTGDILRAAVAAETE
                     VGLRVKSFMDAGNLIPDDVMLDVIRERLSQPDAKAGFVLDGFPRTVVQANALLDLLLE
                     AGTPIERAVLIEVPDEAIIERIESRLSCPTCGRVYSMKTRKPQFDHVCDVDNTPLVRR
                     SDESEDVVRKRLDVYREQTMPVVEFYADRGLLRRVDGVGEVAEVTDRIAA"
BASE COUNT          623 a         1093 c         1186 g          586 t
ORIGIN      
        1 cacatctatg cgcaggtgat cgacgacacg gccggcgcga cgctggccgc cgcctcgtcg
       61 atgaagttga gcgtggacgg cgacaagaag tccgccgcac gcaaggtcgg cgagatggtc
      121 gccgaagccg cgaagcagaa gggtgtctcc aaggtcgtct tcgaccgcgg gggcaacagg
      181 tatcacggcc gcgtcaagga ggtcgccgaa ggcgcccgcg cggcagggct ggagctttag
      241 gaggaatacg tggtcgagcg acgcaggtcg ggtaaggacg aggacgccgg tcgtcccgcg
      301 ctggaagagc gcgtggtgtc catcaaccgc gtggccaagg tggtcaaggg cggccgccgg
      361 ttctccttca ccgcgctcgt cgtcgtcggc gacaaggacg ggaaggtcgg gatcgggtac
      421 ggcaaggcga aggaggttcc gaccgcgatc cagaaaggcg tggacatcgc gcggcgctcc
      481 atgttcaccg tgccgatggt cggccagacg atcccgcacg aagtgatggg cgagctgggc
      541 gccggacgcg tgctgctcaa gccggccgcc ccggggaccg gcgtgatcgc cggcggtccg
      601 gtgcgtgccg tgatggagtg cgcgggaatc cacgacgtcc tttcgaagtc cttggggtcg
      661 gggaacgcga tcaacatgat ccacgccacg gtcgaaggtt tgaagcgcct tcgccggccg
      721 gagacggtgg ctgcgacccg cggattggac attcgccgcg tcgctccccg ccgcatgctc
      781 gaggcggccg gcgtgtccac cgacgccatc gaccggctcg agcgtcagga cgagcgtgcc
      841 gccgcggccg aacccgcccc ggcagccggc acgtccgccc cggaagcgcc caccgccgaa
      901 gctccggcca ccgaggcagc ggccggaggt gagagctgat ggcgaagctc aaggtcactc
      961 aggtcaagag cgtgatcggg acgacgcagg acatgcgcgc caccgtccgt tcgctcggac
     1021 tgcaccgcat ccgtgacgag gcgatcgtgg atgatcgccc ggagatccgc ggaatgatca
     1081 acaaggtcaa gcacctcgtg aaggttgagg aggtccgctc gtgaagctgc atcatctgcg
     1141 tcccgcgccg ggcgccaagc gcccgaagaa gcgctacggc cgcggcgagg cgtccggcca
     1201 cggaaagacg gccggccgcg gaaccaaagg ctcgcgcgcg cgcggcgagg tccacccctg
     1261 gttcgaaggt gggcagatgc cgctgcaccg ccggattccc aagctcggcg ggttcacgcc
     1321 gcgaaccagg accgagtacc gtgaagtgaa cgtggatcgc ttgaacgagt tcgggtcggg
     1381 cgcgaccgtc tcccccgagg acctgatcgc gaagggcttg ctccgcaagg gcaagcagcc
     1441 ggtcaaggtg ctgggtcggg gcgagctcac cgtcgcgctg accgtcaagg cgaacgcctt
     1501 cacggcgggc gcccgtcaaa agatcgaatc ggccgggggc cgcgccgagg tcctctaagg
     1561 agcgcacatg ttcagcgcgt ttcgcaacgc attcaaagtc ccggatctgc gcaacaagat
     1621 cctgttcacg atcttcgtga tcgctctgta ccggctcggg tcccatattc cggtgccggg
     1681 catcgatttc gcgaaggtcg aggagctcaa ggcgcaggca agccagggtg gcatctactc
     1741 gctgatcaac ctgttctcgg gtggggcgct cacgcagttc gcgatcttcg cgctgggcat
     1801 catgccctac atcacttcgt cgatcatcat gcagctgttg acggtggtga tcccgcgcct
     1861 ggaggcctat cagaaggaag gcgaggccgg gcagaagaag atcacccagt acacgcgcta
     1921 catgacggtc gttctcgcgc tgctgcaatc gctgggcatc gtggtgaccg ccaacagcgg
     1981 gcggctcttc ggtgaaggta tccgcgacct gttcgggccg aatctcacat cagggcgaac
     2041 gttcatcgtc gttctcacgc tgactgccgg aacggccatg atcatgtggc tgggcgagct
     2101 gatcacgcag cgcggcatcg gaaacggcat gtcgatcctg atcttcgcct cgatcatctc
     2161 gcggttgccg gccgaatcga ccgctctttg gaccaacgcg ggcaagctga aggcgctagt
     2221 gttcctcggg gtcggcatct tgatcatcgt ggcggtggtg ttggtcgaac aagcccagcg
     2281 aaggatcccg gtgcaatacg ccaagcgtgt ggtcgggagg aagatgtacg gcggaacctc
     2341 gacctacatc ccgctcaagg tgaaccaggc cggcgttatc ccgatcatct tcgcctcgtc
     2401 gctgctgtac ctgccggtgc ttgcatcgac gatcgtgcag agcgcgggct tccagcggtt
     2461 cgtgaaccgg ttcctcgaga acggctcgca ccccgcgtac atcctgatct acttcctgct
     2521 gatcatcttc ttcacgtact tctacacctc gatcaccttc aacccggtcg acgtcgccga
     2581 caacatgaag aagtacggcg ggttcatccc cgggatccgg cccggccggc ccacggcgga
     2641 atacctcgac cacatcctga ctcggatcac gctgcccgga tcgttcttcc tgggcatcat
     2701 cgcggtcatg ccgttcatcc ttcaggggat ctggaacatc acctcgttcc cgttcgcggg
     2761 gacctcgatc ctgatcatcg tcggtgtcgc cctcgaaacg aacaagcaga tcgagtcgca
     2821 gctgatgatg cgtcactacg aaggtttctt gaagtagccg tgcggatcgt gatgctgggc
     2881 ccgccggggg cggggaaggg gacccaagca gcgatgctgg ccgacaagtt cggggtgccg
     2941 cacgtctcga cgggcgacat cctgcgtgcc gcggttgcgg ccgagaccga ggtcggtctg
     3001 cgcgtcaaga gctttatgga tgcgggcaat ttgatcccgg acgacgtgat gctggacgtg
     3061 atccgcgagc gactgtcgca gcccgatgcg aaagcaggat ttgtgctgga cggctttccg
     3121 cggaccgtgg tgcaggccaa cgcgctgctg gacctgctgc tggaagccgg cacgccgatc
     3181 gagcgtgccg tgctgatcga ggttcccgac gaggcgatca tcgagcgaat cgaatcgcgc
     3241 ctgtcgtgcc cgacctgcgg ccgcgtctac agcatgaaga cgcgcaagcc gcagttcgat
     3301 catgtgtgcg acgtggacaa cacgccgctg gtgcgccgca gtgacgaatc cgaggacgtg
     3361 gtgcgcaagc ggctcgacgt ctaccgggag cagacgatgc cggtcgtcga gttctatgct
     3421 gatcgcggct tgctgcggcg ggtggacggc gtcggcgagg tcgccgaggt gaccgaccgc
     3481 atcgccgc
//
LOCUS       DATLFO010000381         5191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_148236, whole genome shotgun sequence.
ACCESSION   DATLFO010000381 DATLFO010000000
VERSION     DATLFO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148236"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4350
                     /locus_tag="VM841_15650"
     CDS             <1..4350
                     /locus_tag="VM841_15650"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HVL91655.1"
                     /translation="IPMGSIGLEASPIGSIALDNILLSSIDADFAALLAGSALQGLAP
                     QTITLRQALADPVVGPRLRALTIRGAGLADTLLRGVQVSSVLLGGLTLNQIVLPGGLS
                     VCQALAQAGYSCAANGVDESRDTVLGLDIASAPMGSIPMGSIPMGSIPMGSIDVLSTP
                     MGSIPLSQIPNVNAVVDCARVNCSTGTLGEAASLVPTAIRAEAVLSDLGAVLDQITLG
                     QLIVGLLARADYPWERLPIDGLQTDAGGPSLVYHVDYDFDCTPFSPPTDITVSPPPGF
                     QYVPGSSLFSFGASSPQAAVDPVAAGGTLRWSALPSNPCGGLEGEEQHVRLSYRMRPG
                     FTLGQFSSKVDAVVFRAAASASGAPVHVTQNFEPNEDPATAPVLAADTLAFGHIATPD
                     DIDLFRVAIPAAPGTRVRFLLSHIAEGQDFDLTVFKPAAPALQSTPIGSIPIGSIPIE
                     DEGADLSGTSRPMQSETLRDSPIGSIPMGSIPIGSLSHNRGNADEAAMIVTAGETGHF
                     TVAVTGFNGSHSNRAYVLRVQTSAPAPLPPCPARTFAFDADPYAGADEATTAGTLPAT
                     VTPDRKTLILVARNRIADLYGPSQAAELMSSLETLAARTEVAGAVLSVDADPGVTGAL
                     KGWDADPCSIDKANGVVRSINDVVTRYRTVATGLRYVVLVGSDEALPMARVADSVSLS
                     NESDYASSLAFTTQNLTKGNATYAAAVRGQILTDDAYTAFTQTPWLDRSLYLPDVSAA
                     RLVETPSEIRGQIAQYIAANGQLAPQTAIVSGYDFLSDGAAAIAAGVDDVLSAGKTAE
                     RLINDQWNKNDLLSRFVRDIISANAHYDHWRAQPASATREEELITTDDAVFGVDPVVG
                     SQLAKRVSFTIGCHSGLSVADSLIANASAAERKILRDFPQTYAQTKAAVYIANTGFGY
                     GDTEANALSERLMTEFARRLRDKWSTIGEKLVYAKHAYFANAGLYGAYDEKVLIEATL
                     YGLPFWRLGPAEPLPAPAQPPATATDPVTGLTVASVSITPQTTRVDSPRGTFWRGGSG
                     VHFMHYRPAQPLETRNVTVPGLRAHGVMIKSLQTQDVPGIDPVLGNPTIAKKANEPER
                     PAVDLVYPANLATLARSRSFGNETATLSLIAGQFRGNSPFDGRGVQRLVKSVTLEVAY
                     SNSGSFIPPSIAQVAVVNAPAGAQVVTDVRDVGGGAVKRVAMLVRHSDDGSWEFVEAV
                     NVAGTSIWIANVTTKSQADTIDVAVMGQDVSGNVGWGTKKGQLHTTGTGVIRPVVTIS
                     SPLDGGSYPRGASVTADYGCYDPAGVISCTGTVPNGTPVDTSTTGTKTFTVRMTNTKG
                     EVLERTVRYTVGFEFVGFLSPVSNYPTLNSASAGSAIPVKWSIKDGQGGFVRDPATVD
                     TIRSQTISCANRRSSIAEDTSTPGKVVVTYDLPKEHFHYDWKTDPSWQGTCRRLFLTL
                     SDKTKHFFDVSFK"
BASE COUNT          841 a         1814 c         1740 g          796 t
ORIGIN      
        1 atcccgatgg gctcgatcgg gctcgaagcc tccccgatcg gctcgatcgc gctcgacaac
       61 atcttgctgt cctcgatcga cgccgacttc gcggcattgc tggccggctc ggccctgcag
      121 gggctcgcgc cacagacgat cacgcttcgg caggcgctcg ccgacccggt cgtcggaccc
      181 cggctccgtg cactcaccat tcgcggtgcc ggcctggccg acacgctgct gcgcggcgtg
      241 caggtgagct cggtcctgct cgggggtctg acgctcaacc agatcgtgct gcccggaggc
      301 ctgagcgtgt gccaggcgct tgcgcaggcc ggatactcgt gcgccgccaa cggcgtcgac
      361 gagagccgcg acaccgtgct ggggctcgac atcgcgagcg ccccgatggg ctcgatcccg
      421 atgggttcga tcccgatggg ttcgatcccg atgggctcga tcgacgtgct gtccacgccg
      481 atgggctcca ttccgctgtc ccagatcccg aacgtcaatg cggtcgtgga ctgcgcgcgg
      541 gtcaactgct cgacgggcac gctcggcgag gccgcgtcgc tcgtgcccac ggccattcgc
      601 gccgaagcgg tgctctcgga cctcggcgcg gtgctggatc agatcacgct cggccagctc
      661 atcgtggggc tgctcgcccg cgccgactat ccgtgggagc ggcttccgat cgacgggcta
      721 caaaccgacg cgggcgggcc gtcactggtc taccacgtcg attacgactt cgactgcacg
      781 ccgttctcgc cgccgaccga catcaccgtc tcgccgccgc cgggcttcca gtacgtgccc
      841 ggctccagcc tgttctcctt cggggcctcg tccccgcagg ccgcggtgga tccggtcgcc
      901 gccggcggaa cgctgcgctg gtcggccctg ccgtcgaacc cgtgcggcgg cctggaaggc
      961 gaggagcagc acgtgcgcct ctcgtaccgg atgcgtccgg gcttcacgct cggccagttc
     1021 tcgagcaagg tggacgcggt cgtgttccgc gcggcggcgt ccgcgtcggg cgcgccggtc
     1081 cacgtgacgc agaacttcga gcccaacgag gatccggcaa ccgcgccggt gctcgcggcc
     1141 gacacgctcg cgttcggcca catcgccacg cccgacgaca tcgatctgtt ccgtgtggcc
     1201 atcccggccg cgcccggaac ccgcgttcgg ttcctgctgt cgcacatcgc ggagggacag
     1261 gacttcgacc tgaccgtgtt caagccggcc gcgccggcgc tgcagtccac gccgatcgga
     1321 tcgatcccga tcggatcgat cccgatcgag gacgaaggcg ccgacctgtc gggcacctcg
     1381 cggccgatgc agtccgagac gctgcgcgac agcccgatcg gttcgatccc gatgggctcg
     1441 atcccgatcg gcagcctgtc gcacaaccgc ggcaatgccg atgaggccgc gatgatcgtc
     1501 accgccgggg agaccggtca cttcacggtc gccgtcaccg gcttcaacgg ctcgcactcg
     1561 aaccgggcct acgtgctgcg cgtgcagacg tcggcgccgg ccccgctgcc gccgtgcccg
     1621 gcccggacct tcgctttcga cgccgacccg tacgcaggcg ccgacgaagc caccacggcc
     1681 gggacgcttc ctgccaccgt gacgcccgac cgcaagacgc tcatcctggt cgcgaggaac
     1741 cggatcgccg acctgtacgg cccgagccag gcggccgagc tgatgagctc gctcgagacg
     1801 ctggccgcgc gcaccgaggt cgccggtgcg gtgctgtcgg tcgatgccga tcccggggtg
     1861 acgggcgcgc tcaaggggtg ggacgccgat ccctgctcga tcgacaaggc gaacggcgtc
     1921 gtgcgatcga tcaacgacgt cgtgacgcgg taccgcacgg tcgcgacggg attgcgctac
     1981 gtcgtgctgg tcggctcgga cgaggcgctg ccgatggctc gcgtcgccga ctcggtctcc
     2041 ctgtcgaacg agagcgacta cgcatcctcg ctcgccttca ccacccagaa cctcaccaag
     2101 ggcaacgcca cctacgcggc ggcggtgcga ggccagatct tgaccgacga cgcctacacg
     2161 gccttcaccc agaccccgtg gctggaccgc tcgctgtacc tgcccgacgt ctccgccgcg
     2221 cgactggtcg agacgccgtc ggagatccgg ggccagatcg cccagtacat cgccgcgaac
     2281 ggacagctcg cgccacagac ggccatcgtc agcggctacg acttcctgtc cgacggcgcg
     2341 gccgcgattg ccgcgggcgt ggacgacgtt ctgtctgccg gcaagaccgc ggagcggctg
     2401 atcaacgatc agtggaacaa gaacgatctg ctgtcgcggt tcgtgcgaga catcatctcg
     2461 gccaacgcgc actacgacca ctggcgcgcg cagccggcgt ccgcgacccg cgaggaagag
     2521 ctgatcacga ccgacgacgc cgtcttcggc gtcgatcccg tggtcggatc gcagctcgcc
     2581 aagcgcgtgt cgttcacgat cggctgccac tccgggctga gcgtggccga ctcgctgatc
     2641 gccaacgcgt cggcggccga gcgcaagatc ctgcgggact tcccgcagac gtacgcgcaa
     2701 acgaaagccg cggtctatat cgccaacacc gggttcggct acggcgatac cgaagccaac
     2761 gcgctgtccg agcggctgat gaccgagttc gcgcgacgtc tgcgcgacaa atggtccacg
     2821 atcggcgaga agctcgtcta cgccaagcac gcgtacttcg ccaacgccgg cctgtacgga
     2881 gcctacgacg agaaggtgct gatcgaggcg acgctctatg gcttgccgtt ctggcggctg
     2941 ggaccggccg agccgctgcc ggcacccgcg cagccgccgg cgaccgcgac ggacccggtg
     3001 accggcctga cggtcgcctc ggtgtcgatc acgccgcaga cgactcgcgt cgactcgccg
     3061 cgcggcacct tctggcgggg cggctcgggc gtgcacttca tgcactaccg cccggcgcag
     3121 ccgctggaga ccaggaacgt gaccgtgccg ggcctgcggg cccacggcgt catgatcaag
     3181 tcgctgcaga cccaggacgt gcccggcatc gacccggtgc tcgggaaccc gacgatcgcc
     3241 aagaaggcca acgagcccga gcgtcctgcg gtcgatctgg tctacccggc gaacctcgcc
     3301 acgctcgccc ggtcgcgcag cttcggaaac gagacggcga cgctgagcct gatcgccggt
     3361 cagttccgtg gcaacagccc gttcgacggc cgcggcgtgc agcggttggt gaagtcggtc
     3421 acgctcgaag tcgcgtactc gaactcgggc agcttcatcc cgccgtcgat cgcacaggtc
     3481 gccgtggtca acgcgccggc cggggcgcag gtcgtcacgg acgtgcgtga cgtcggcggc
     3541 ggcgcggtca aacgcgtcgc catgctcgtc cggcactccg atgacggctc atgggagttc
     3601 gtcgaggccg tcaatgtcgc ggggacgagc atctggatcg ctaacgtgac caccaagtcg
     3661 caggccgaca ccatcgacgt cgccgtgatg ggccaggacg tgagcggcaa cgtcggctgg
     3721 ggcacgaaga agggccagct gcacaccacc ggaaccggcg tcattcgccc ggtcgtgacg
     3781 atctcgtcgc cgctggacgg cggctcctac ccgcgcggcg cgagcgtgac cgcggactac
     3841 ggctgctacg accccgcggg cgtcatctcg tgcaccggca cggtgccgaa cggcacgccg
     3901 gtggatacct cgacgaccgg caccaagacc ttcacggtgc gcatgaccaa caccaagggc
     3961 gaggtgctcg agcgcacggt ccgctacacg gtcggcttcg agttcgtcgg attcctgtcg
     4021 ccggtgagca actatccgac gctcaactcc gcgtcggccg gctcggcgat cccggtgaag
     4081 tggagcatca aggacggcca gggcggcttc gtgcgcgacc cggcgaccgt ggacacgatc
     4141 cggtcgcaga cgatctcgtg cgcgaaccgg cggtccagca tcgccgagga cacgagcacg
     4201 ccgggcaagg ttgtggtcac ctatgacctg cccaaggagc acttccacta cgactggaag
     4261 acggacccgt cgtggcaggg cacctgccgc aggctgttcc tgaccttgtc cgacaagacg
     4321 aagcacttct tcgacgtctc gttcaagtaa cgggaccgag acgcggcccc cggagatttc
     4381 tgcaggtttc cgggggctgc ggctcgagca gcaggggagc cccttcgggg tcgcgttcac
     4441 gcgtggcagc aaatcgggag acgagcgcga cccgagccaa ggcgatgctt gccgcgccgg
     4501 cgcagccggc cccgtgcgtt gatctctcgg cgaaattacg attgaaggaa atctcctcag
     4561 aagtttaatt taatctttcg aaaacctttg gtcgggcagg atccctcttg tgccgaggag
     4621 aagtggagcc ccttgacgac gagtctgcgc ctccttctct ccccgccggg ttctcagccg
     4681 gctgccctga taggggagga ggcgcggcct gtctccttgg gggcaccgcc ccgggtggga
     4741 acgaaggacg cgcatgagga ccggaccgaa accgaaggcc gcaccgacgg tggcgggcgc
     4801 cacgagctcg ctcccgcaag acggcgcaac ccggccgatc cggtacggct tgcttggccg
     4861 tttcgggcgc agttgctctg atgtcgcgaa tgtccgcaac cctcgctgcc accctccgtg
     4921 ttcggcggac gagacctggg agggtttccc ggctgctgtg acgaaaaggt agtggggcgg
     4981 gatccggccc gggcaggtac cattggccgc cgccttgaag gcgagaggag gagcgcgaag
     5041 gtgtcgaagg aagataagaa gctcaaggtc tacgccaaaa agcagaccgg agcgaacaaa
     5101 aagaagaagc tgaagctgcc gaacatcaag ttcaaggcgc tgatcgggcc gatgctgctc
     5161 accctcgcgc tggtgctgat cggtgtctgg t
//
LOCUS       DATLFO010000382        10677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_148296, whole genome shotgun sequence.
ACCESSION   DATLFO010000382 DATLFO010000000
VERSION     DATLFO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148296"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VM841_15655"
     CDS             complement(<1..1242)
                     /locus_tag="VM841_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91656.1"
                     /translation="MRRSFAFLVLALLVLTTLSVADRAPASMTLECNEGICRREPPPV
                     APSCTQCIPASEGTTVIRGLVIDPKGVPIVGASVYWTYAQTTTDAAGRYVIATWASKP
                     VTLIATHPRHVWKATAVTDPLESAVSGVNVLLPYALVPSLSPFAVPRGSASSIRVDAW
                     AGASVAGGRLVGRAGGTDRALAPVVSHPDGSSAWRGSIDVPPNAWDGTYAYSICILDA
                     ASPAQSCHGAPPGALMSEIVTLSYVVDGIAPTIVKPRPNGDGATAAPLVGAAVTDPGP
                     AGIDASSIVLTIDGAAVPATFTAASGSVSHQASSLAPGTHTARLVAADRAGNQTALSW
                     QFVVQPVVDAGTIAASSDPSTCPLAGVALHSLNGRAVTVYQGSISVSPIPPPDAQVAI
                     EAARFAPPRSVLYAEDLTFSWD"
     gene            1404..4526
                     /locus_tag="VM841_15660"
     CDS             1404..4526
                     /locus_tag="VM841_15660"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVL91657.1"
                     /translation="MATMTTCAKCGAPLPADARFCAQCGAPVDASTTEERKVVTVLFA
                     DLAASTELAATLDPERFREVMAAFYNAITAELSNMRGRAEKFAGDAVMAVFGVPQAHD
                     DDAVRAVHAALRIRERIAKLSKQLALPVELSVRIGVNTGPVATGTGPADQFLVSGAPV
                     NMAARLQQAAEPGEVLCGDTTHMLSSHAVVYGGPRTIRAKGFDDSVRAWPAEMPTGRT
                     ATRRQLPMVGRRRELALMLDSFERARETGRAHLVTLLGEAGVGKSRLIESFIEALPDD
                     ARVMSGRATEFEEDPTFSALAEMIRRELGVKREAPREEVSKLLLDMVAGCCDPTESEK
                     TAARLGLAIGLGENTEGNPYEVAEVRTGLMSLLEGLARTGPVALVFEDLEIARSGFLD
                     LIEQLVVRAKRLPLLIVCVARDSLLEMRPTWGGGLPDALTIRLEPLGDHDGKDLARAA
                     GPDLHEEDFERIARHAGGNPFFIVETAVMLASEHHDHTHNVPHSALLPPTVQAVVAAR
                     LDHLPAKARALVRKASVFPRAVVNEIELSLIADLDEETLQLLEDEDVLIRDDKRTGVW
                     RFRHETLRDVAYETLPKRERMRLHLAVAEGLPDPERYPSVVAYHLEQAARASLDLTPN
                     DRTLADRAVDALQHAGDVQRRRIQSRIAIELYERGLALAGPESSWGVREARMLSGIGE
                     ARYWRGEYDEADAVFGRALALAGPDPWVRAHANRFAGDIELNVRGNLSRAEWTFDEAL
                     SAARELSDPWAISRTLLVAAWAPYARGGDLGPSRAMLEEALEAARAGDPRDRWAEARA
                     LTFLAIVTIHERSAAEVLDLAMQSLEVGRDLDDAFTIAVAHQTVANAMRRLGRLDEAW
                     TLADQATRSFVDLGAPWERASAQGDRGNIARLLGRFDEAETDLREALATCREIKERTL
                     AAWTSSELARVLIARGELAEAESLLRHPSTLFAVTEVGSRVALVNSSGLVALATGNTD
                     VARERFAEALSLERERGWPNQTAAQTWAVASLCGAGAAGGTQAVDGARSRLEQMEWRL
                     VLERPRDALA"
     gene            complement(4523..4900)
                     /locus_tag="VM841_15665"
     CDS             complement(4523..4900)
                     /locus_tag="VM841_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVL91658.1"
                     /translation="MNNDTRRTSVLIVEDEIEFRFLLRVVLDRVAGCEVVAESVDCAD
                     AIVQAAAHQPGLIFLDVHMPKGNGLHAVEQIDRASPRSTVIVMSSDPETRIPATRMGL
                     RWVDKARLMRELPGILERAPASG"
     gene            5129..6223
                     /locus_tag="VM841_15670"
     CDS             5129..6223
                     /locus_tag="VM841_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91659.1"
                     /translation="MTTHASENDRRSRGPNRRTPLLLAAGLACLLAIAGASGAAPRDI
                     ASSSAAALGRAIVSEPLQRPSIRVSPPASPGPSASATAAVATPAAAAPAAETPDSRPT
                     RPAARARSAAAGAPVDGKGMWIWHYGRIAGGNHETIVRHAENLGLTHIYVRVGSGYSG
                     LDTLAQVAPVIPVAHRAGIKVIAWYFPYYNDIQADVRRSVEAMSTQVRGESFDGFAAD
                     IEPAKGADFSAETAAAYSRALRAALPDRHLIAVPMRPTKSTIASGIFDAIMPHYDAVA
                     PMTYWGRFDPAETVRYTIEYLSRYGKPVSPVGQTYDMGPEGGPRGNPPPHQTWAFMKA
                     AGAAGAVGVSFWSWQHATSPEFDAIRKYRW"
     gene            6392..8572
                     /gene="katG"
                     /locus_tag="VM841_15675"
     CDS             6392..8572
                     /gene="katG"
                     /locus_tag="VM841_15675"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HVL91660.1"
                     /translation="MSESENPAIPAPDPKPTRPRTNRDWWPNQLDLSVLHQHAPASNP
                     MDPGFDYADEFKKLDLDALKQDIVQVMRTSQDWWPADYGHYGPLFIRLTWHAAGTYRT
                     ADGRGGAGDGSQRFAPLNSWPDNANLDKARRLLWPVKKKYGNKISWADLLVLAGNVAL
                     EDMGFKTFGFGFGREDIWQPEEIFWGTEDTWLGDERYAGDRELQGPVGAVQMGLIYVN
                     PEGPSGNPDPLAAARDIRETFKRMAMNDEETVALIAGGHTFGKTHGAGDPSLVGPEPE
                     GAPLENQGIGWKSSFGSGKGKDTITSGLEGAWTPTPIKWDNSFFQTLFGHEWELTKSP
                     AGAKQWKPKDGAAADLVPDAHDPAVRHAPMMATTDLALRFDPQYEKISRRFMENPDQL
                     AEAFAKAWYKLLHRDMGPVARYLGPWVPEPQLWQDPVPAVDHALIDDADIASLKDKLL
                     GSGLSVQQLVTTAWAAASSFRTTDKRGGANGARLRLEPQKNWEVNQPAELASVLQALE
                     QIQSEFNATASGGKKVSLADLIVLGGCAAVEKAAKDVDVHVTVPFTPGRTDASQEMTD
                     VEAFAPLEPTADGFRNYVRPGEKLSPETLLVDRAYLLNLSAPEMTVLVGGMRTLGATY
                     GGAKHGVLTDKPGTLTNDFFVNLLEMIEWTVSSSEEGVYEGRPFGSEEVKWTATAVDL
                     VFGANSQLRALAEVYASDDAKEKFVRDFVAAWNKVMMNDRFDVK"
     gene            complement(8643..8921)
                     /locus_tag="VM841_15680"
     CDS             complement(8643..8921)
                     /locus_tag="VM841_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91661.1"
                     /translation="MVLAKARVESFEKFWQVFSTIGAEHRAKHGSKGSQVYQDSGDPN
                     TVWVMFNWDRSGYEGFIADPKTKEIFQKGGLQGPPEPIFLDHSGEADA"
     gene            complement(8956..9825)
                     /locus_tag="VM841_15685"
     CDS             complement(8956..9825)
                     /locus_tag="VM841_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91662.1"
                     /translation="MILIGTKNGLVTSDGTALLEGDVTHVIDGEDGAWVLTTEQILQG
                     EPDALRAVAPLDGIEANCLLPSRHGLFVGAADARLYRLDGSSLQRVESFDQVQDRDTW
                     HTPWGGPPDVRSMTEAPDGTIYAGVHVGGIVRSRDGGETWTPTIDIDTDIHQVITHDD
                     LVIAPGWNALHVSEDAGETWTEHRDGFDHTYMRAVAIAGDALLVSAAYSHAGKDAGVY
                     RRDLRGDGPFERIEPVHPSEHNIDTFWLAANGSQAAFVTAEGELYRSTDRGRTWSRAA
                     RGLALPRCLALRE"
     gene            9908..>10677
                     /locus_tag="VM841_15690"
     CDS             9908..>10677
                     /locus_tag="VM841_15690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVL91663.1"
                     /translation="MVSESRIDRVTGAAFVGMVAGIAGNVVAVKYVARAGDQDPMWAA
                     ASRFALATAIFLVIAIAVRAAFPVGRALAGALLYGSLTFGGFFVFAYLGLQRAPVGIA
                     SVVLATGPLLTFAFALAHRQERFRWDSLIGGAAAVAGTALVFVSGLDQGVPGWSLLAI
                     LAASACAAEGTVIVKAFPPMHPVMRNAIGTGVGTAILALMIPLFGESIAVPSEPQTWV
                     AQAYLITFGTVGVFGLYLFLLSRWTASAVSYEFVLAPLA"
BASE COUNT         1702 a         3674 c         3660 g         1641 t
ORIGIN      
        1 gtcccagctg aaggtcagat cctcggcgta gagcaccgac ctgggcggcg cgaagcgggc
       61 ggcctcgatc gcgacctgcg catccggcgg cgggatcggc gagaccgaga tcgatccctg
      121 gtagaccgtg accgctcgtc cgttcagcga atgcagcgcc acgccggcaa gcggacaggt
      181 cgagggatcc gacgacgcgg cgatcgtccc ggcatccacc accggctgca cgacgaactg
      241 ccacgacaag gccgtttggt ttcccgcccg gtcggccgcg acgagccgcg cggtatgcgt
      301 acccggcgcg agcgagcttg cctggtgcga taccgagccg gacgcggcgg tgaaggtggc
      361 cggcaccgcc gcgccgtcga tcgtgagcac gatcgacgag gcatcgatcc cggccggacc
      421 gggatcggtc accgccgcgc cgaccaaggg cgcggccgtt gctccatcac cgttcggacg
      481 cggcttgacg atcgtcggag cgattccgtc cacgacgtag gagagcgtca cgatctcgct
      541 catcaatgct ccgggaggcg cgccgtggca ggactgcgcc ggggacgcgg cgtccagaat
      601 gcagatcgaa taggcgtagg tgccgtccca ggcattcgga ggaacgtcga tcgagccgcg
      661 ccatgccgac gagccgtcgg gatgtgacac gaccggcgcg agcgctcggt cggttccccc
      721 ggcgcggccc acgaggcgac cgcccgccac cgatgcgccc gcccacgcgt cgacacggat
      781 ggatgacgcg gaaccccgcg ggaccgcaaa cggcgacagc gacggcacca gcgcgtatgg
      841 caacaggacg ttgaccccgg agaccgcact ctccaacgga tcggtcaccg cggtcgcctt
      901 ccagacgtgc ctcgggtgtg tcgcgatcag cgttaccggc ttggatgccc aggtcgcgat
      961 cacgtatcgc cccgctgcgt cggtggtggt ctgcgcgtag gtccaataca cggaggcgcc
     1021 cacgatcgga acgcccttgg gatcgatcac gagcccgcga atcaccgtgg tgccctccga
     1081 cgccggaatg cactgtgtgc acgacggggc cacggggggc ggctcgcgcc ggcagatccc
     1141 ctcgttgcat tcgagggtca tgctggccgg ggcgcggtca gccacggaca gcgtggtcaa
     1201 aaccaaaagc gccaggacga ggaacgcaaa cgagcgacgc atgatcgact cccctcggtc
     1261 gagcgccacg ggacgcgcta tgcggctcga ccctcacccg aatattgatg ctgatatatc
     1321 tgctttacga ggtttggtca agatgttcct cttgcgaatt tcttgaaagg cgggtccgac
     1381 tggtcctcta tgctcctggt cgtatggcga ccatgacgac ctgtgcgaag tgcggcgcgc
     1441 ctctgcctgc cgacgcccgc ttctgcgccc agtgtggcgc tcccgtcgat gcttcaacca
     1501 ccgaggaacg caaggtcgtc acggtgctgt tcgccgatct cgccgcctcg accgagcttg
     1561 cggcgacact cgatcccgaa cggttccgcg aggtcatggc cgccttctac aacgcgatca
     1621 cggcggagct gtcgaacatg cgcggccggg ccgaaaagtt cgccggcgac gcggtgatgg
     1681 cggtgttcgg cgtgccgcag gcgcacgacg acgacgcggt acgcgcggta cacgcggcgc
     1741 tgcgcatccg cgagcggatc gcgaagctgt cgaagcagct cgcgcttccc gtcgagctct
     1801 cggtgcggat cggcgtgaac accgggccgg tcgcgaccgg caccggcccg gcggatcagt
     1861 tcctggtcag cggcgctccg gtcaacatgg ccgcgcgtct tcaacaggca gccgaaccgg
     1921 gcgaggtgct gtgcggcgac accacgcaca tgctcagctc gcatgcggtc gtctacggcg
     1981 ggccgcgcac catccgcgcg aaaggcttcg acgacagcgt tcgcgcatgg ccggccgaga
     2041 tgccgaccgg ccgcaccgcc acgcgccgcc agcttccgat ggtcggacgc cgccgcgagc
     2101 tcgcactgat gctcgattcg ttcgagcgtg cgcgcgaaac cggccgcgct catctggtga
     2161 ccctgctcgg agaagccggg gtcggcaaga gccgcttgat cgagagcttc atcgaggcgc
     2221 tgcccgacga cgcgcgcgtg atgagcggcc gcgcgaccga gttcgaagag gacccgacgt
     2281 tctcggcgct ggccgagatg atccgccggg agctcggggt caaacgcgaa gcgccgcgcg
     2341 aagaggtctc caagctgctg ctggacatgg tcgcgggctg ttgcgatccg accgagagcg
     2401 aaaagaccgc ggcccggctg ggtctggcga tcgggctcgg cgagaacacc gaaggcaacc
     2461 cctacgaggt cgccgaggtc cggacgggct tgatgagcct gctcgaaggc ctcgcccgga
     2521 ccgggccggt cgcgctggtg ttcgaggatc tggagatcgc gcgatcgggg tttctcgatc
     2581 tgatcgagca gctcgtggtg cgcgcgaagc ggctgccgtt gctgatcgtg tgcgtcgcgc
     2641 gcgactcact gctcgagatg cgcccgacct ggggcggcgg cctgccggac gcgctcacga
     2701 tccggctcga gccgctcggc gatcacgacg gcaaggacct ggcgcgtgcg gccggcccgg
     2761 atcttcacga ggaagacttc gaacgcatcg cgcggcacgc gggtggcaat ccgttcttca
     2821 tcgtcgagac cgcggtgatg ctcgcctcgg agcaccacga tcacacccac aacgtgccgc
     2881 acagcgcgct gctgccgccg acggtgcaag cggtggtcgc cgcgcgtctg gatcacctgc
     2941 cggccaaggc acgcgccctg gtccgcaagg cctcggtctt cccgcgcgcg gtcgtcaacg
     3001 agatcgagct cagcctgatc gccgacctcg acgaggaaac gctgcagcta ctcgaggacg
     3061 aggacgtgct gatccgcgac gacaagcgta cgggcgtgtg gcgtttccgt cacgagacgc
     3121 ttcgcgatgt ggcctacgag acgctgccca agcgcgaacg aatgcgcctc cacctggccg
     3181 tcgccgaagg cctgcccgac ccggaacgct atccatcggt cgtggcctac cacctcgagc
     3241 aggccgcgcg cgcatcgctc gacctgaccc cgaacgaccg cacgctcgcc gaccgggccg
     3301 tggatgcgct ccagcatgcc ggcgacgtgc agcggcggcg catccagtcg cgcatcgcga
     3361 tcgagctcta cgagcgcggc ctcgccctgg ccggtcccga gtcctcgtgg ggcgtgcgcg
     3421 aggcgcggat gctcagcggc atcggcgagg cgcgctattg gcgcggcgag tacgacgagg
     3481 ccgacgcggt gttcggccgt gcgcttgcgc tggcgggtcc ggacccgtgg gtgcgagcgc
     3541 acgcgaaccg gttcgccggc gacatcgagc tcaacgtgcg cggcaacctc tcgcgcgccg
     3601 aatggacctt cgacgaggcg ctgtcggccg cgcgcgagct cagcgacccg tgggcgatct
     3661 cgcgcacgct gctcgtcgcc gcatgggcgc cgtacgcgcg cggcggcgat ctcgggccct
     3721 cccgcgcgat gctcgaagag gcgctcgagg cagcacgcgc cggcgatccg cgcgaccgct
     3781 gggccgaggc gcgcgcgctg acgttcctgg ccatcgtgac gatccacgag cgcagcgccg
     3841 ccgaggtgct ggacctggcg atgcagtcgc tcgaggtcgg acgggacctc gacgacgcct
     3901 tcaccatcgc ggtggcgcat cagaccgtgg caaacgccat gcgccgtctc ggccgcctcg
     3961 acgaagcttg gacgctggcg gaccaagcga cacgctcgtt cgtcgatctc ggcgcgccgt
     4021 gggagcgggc cagcgcgcaa ggcgaccgcg gcaacattgc gcgtctgctc gggcggttcg
     4081 acgaagccga gacggacctg cgcgaggcgc tggcgacctg ccgcgagatc aaggagcgca
     4141 ctctcgcggc ctggacgtcc agcgagcttg ctcgcgtgct gatcgcgcgc ggcgagctcg
     4201 ccgaggccga aagcctgctg cggcacccct cgacgctgtt cgcggtaacc gaggtcggat
     4261 cgcgggtcgc gctggtcaac agcagcgggc tggtcgcact cgccaccggc aataccgacg
     4321 tcgcgcgcga acggttcgcc gaggcgctgt cgctcgagcg agaacgcggc tggccgaacc
     4381 agaccgccgc gcaaacatgg gcggttgcgt cgctgtgcgg cgcgggcgcg gccggcggaa
     4441 cgcaggcggt ggacggggca cgctcgcgcc tcgagcagat ggagtggcgg ctcgtcctcg
     4501 agcggccgcg cgacgcactc gcttagccgc tcgcgggagc gcgctccagg atccccggca
     4561 gttcgcgcat caagcgggcc ttgtctaccc agcgcagccc catccgcgtc gcgggaatcc
     4621 gcgtctcggg atcgctcgac atcacgatga cggtcgaacg cggactcgca cggtcgatct
     4681 gctcgaccgc atgcagacca ttgcccttgg gcatgtgaac gtcgagaaag atcaagcccg
     4741 gctgatgcgc ggcggcctgc acgatcgcgt cggcgcaatc aaccgattcc gcgacgacct
     4801 cgcaccccgc aacccgatcg agcaccacgc gaaggagaaa gcggaactca atttcgtcct
     4861 cgacgatcag aacgctggtg cggcgggtgt cgttgttcat gcatgcatca tcggccgtcg
     4921 aacctgtggc cgcggtccga tccttgtaaa aagattgcaa agacgtccgc tttcgtccgt
     4981 tctcgtcgca acccaatctg agtaatcgtc ctcgggtgct tgcatcgtct ctgcgcttca
     5041 tccgcagaat gcgctcgctt cggcttcaag acgcacttgc acggcgtcga ggtcacaggg
     5101 gcggcgactc tttcgaaagg acttgacctt gacgacgcac gcatccgaaa acgaccggcg
     5161 ttcgcgggga ccgaaccggc gcacgccgtt gcttctcgcc gcgggcctgg cctgcttgct
     5221 cgcgatcgcc ggggcctcgg gcgccgcgcc gcgcgacatc gcgagcagct ccgccgcggc
     5281 gctcggccgc gcgatcgtga gcgagccgtt gcagcgcccc tcgattcgcg tctcaccccc
     5341 ggcttcgccc gggccctccg cctccgcgac agccgcagtc gcgaccccgg ccgctgcggc
     5401 accggcggcc gagacgcccg actcgcgtcc cacccgcccc gccgcgcggg ctcgctcggc
     5461 ggcagcgggc gcgccggtcg acggcaaagg catgtggatc tggcactacg gacgcatcgc
     5521 gggcggcaac cacgaaacga tcgtgcggca cgccgagaat ctcgggctga cgcacatcta
     5581 cgtgcgcgtc ggctcgggct actcgggcct cgacacgctc gcgcaggtcg cgccggtgat
     5641 cccggtcgcg catcgcgccg gcatcaaggt gatcgcctgg tacttcccgt actacaacga
     5701 catccaggcc gacgtccggc gcagcgtgga ggcgatgagc acgcaggttc gcggcgaaag
     5761 cttcgacggc ttcgccgccg acatcgagcc cgccaagggc gcggacttct cggcggagac
     5821 cgccgcggcc tactcacgcg cgctgcgcgc ggcgctgccg gaccgccatc tgatcgcggt
     5881 gccgatgcgg ccgacgaagt cgacgatcgc gagcggcatc ttcgacgcga tcatgccgca
     5941 ctacgacgcc gtcgcgccga tgacctactg gggccgcttc gacccggccg agaccgtgcg
     6001 ctacacgatc gagtacctgt cccgctacgg caagccggtc tcgcccgtcg gccagaccta
     6061 cgacatgggt cccgagggcg gtccgcgcgg caacccgccg ccgcaccaga cgtgggcgtt
     6121 catgaaggcc gcgggggccg cgggtgccgt gggcgtgtcg ttctggtcgt ggcagcacgc
     6181 gacgagcccg gagttcgacg cgatccgcaa gtaccgctgg tagcccgctc ggcggaccgt
     6241 ccgcgccgcg cacacgcagc ccccgccgga tccacggcgg gggctgcgtg gcgagcggac
     6301 cgagacgcgg actatccttc ttcttcgccc acgcgatcga ttcgctcgag cgcgaggccc
     6361 cgacaaccaa aagcgatcaa ggaggaccgc cgtgtccgag agcgagaacc cagcaatccc
     6421 agcaccggat ccgaagccga cgcggccgcg caccaaccgc gactggtggc cgaaccagct
     6481 cgacctgtcc gtgctacacc agcacgcgcc ggcgtccaac ccgatggacc cgggattcga
     6541 ctacgcggac gagttcaaga agctcgactt ggatgcgctc aagcaggaca tcgtccaggt
     6601 gatgcgcacg tcccaggact ggtggccggc cgactacggc cactacggcc cgctgttcat
     6661 ccgcttgacc tggcacgcgg ccggcacgta ccgaaccgcg gacggccgcg gcggcgcggg
     6721 tgacggctcg cagcgcttcg cgccgctcaa ctcctggccc gacaacgcga atctcgacaa
     6781 ggcgcggcgt ctgctgtggc cggtcaagaa gaagtacggc aacaagatct cgtgggcgga
     6841 cctgctcgtg ctcgccggca acgtcgcgct cgaggacatg ggattcaaga cgttcggctt
     6901 cggcttcggc cgcgaggaca tctggcagcc cgaggagatc ttctggggca ccgaggacac
     6961 ctggctgggc gacgagcgct acgccggcga ccgtgagctt cagggtccgg taggcgcggt
     7021 ccagatgggc ttgatctacg tcaatccgga aggcccgagt ggcaacccgg atccgctcgc
     7081 cgccgcgcgc gacatccgcg agacgttcaa gcgcatggct atgaacgacg aggagacggt
     7141 cgcgctgatc gccggtggcc acacgttcgg caagacgcac ggcgccggcg acccgagcct
     7201 cgtcggcccc gagcccgaag gcgcgccgct cgagaatcaa ggcatcggct ggaagagcag
     7261 cttcggcagc ggcaaaggta aggacacgat caccagcggc ctggaaggcg cgtggacgcc
     7321 gacgccgatc aagtgggaca acagcttctt ccagacgctg ttcggccacg agtgggagct
     7381 gaccaagagc cccgcgggcg cgaagcagtg gaagcccaag gacggcgcgg ccgcggatct
     7441 cgtcccggac gcgcacgacc cggccgtgcg tcacgcgccg atgatggcca cgacggacct
     7501 ggcgctgcgc ttcgaccccc agtacgagaa gatctcacgc cgcttcatgg agaatcccga
     7561 tcagctcgcc gaggcgttcg cgaaggcgtg gtacaagctg ctgcaccgcg acatgggtcc
     7621 ggtggcgcgg tacctgggcc cgtgggtgcc ggagccgcag ctgtggcagg acccggttcc
     7681 ggccgtcgat cacgcgctga tcgacgacgc cgacatcgcc tcgctcaagg acaagctgct
     7741 cggctcgggc ctttcggttc agcagctggt caccaccgcg tgggcggcgg cgtcgagctt
     7801 ccgcacgacc gacaagcgcg gcggcgcgaa cggcgcgcgg ctccgtctgg agccgcagaa
     7861 gaactgggag gtcaaccagc cggccgagct cgcgagcgtg ctccaggcgc tcgagcagat
     7921 ccagagcgag ttcaacgcga cggcctcagg cggcaagaag gtctcgctgg cggacctgat
     7981 cgtgctgggt ggctgcgccg cggtcgagaa ggctgccaag gacgtggacg tgcacgtgac
     8041 ggttccgttc acgccgggcc gcaccgacgc gtcacaagag atgaccgacg tcgaggcgtt
     8101 cgcgccgctc gagccgacgg cggacgggtt tcgcaactac gtccgtcccg gcgagaagct
     8161 ctcgcccgag acgctgctgg tggaccgggc ctacctgctc aacctcagcg ctcccgagat
     8221 gaccgtgctg gtcggcggca tgcggacgct gggcgcaacc tacggcggag cgaagcacgg
     8281 agtgcttacc gacaagccgg ggacgctgac caacgacttc ttcgtcaacc tgctcgagat
     8341 gatcgagtgg acggtgtcat cgtcggagga aggcgtctac gaaggccgtc cgttcggatc
     8401 tgaggaggtc aagtggaccg cgacggcggt ggacctggtc ttcggcgcca actcgcagct
     8461 tcgcgcgctg gccgaggtct acgcgagcga cgacgccaag gaaaagttcg tgcgtgactt
     8521 cgtcgccgcg tggaacaagg tcatgatgaa cgaccgcttc gacgtgaagt agctccgctc
     8581 cgttcgcggc ctggtggccg gcccggaaac ggggccgcca ccaggccgct ttttcgtgcg
     8641 ctctaggcgt cggcttcgcc gctgtgatcc aggaagatcg gctcgggcgg gccctgaagg
     8701 cctccctttt ggaagatctc cttggtcttg ggatcggcga tgaatccttc gtagccgctg
     8761 cgatcccagt tgaacatgac ccagaccgtg ttgggatcgc cggagtcttg gtagacctgc
     8821 gagcccttcg atccgtgctt ggcgcggtgc tcggcaccga tcgtgctgaa cacctgccag
     8881 aacttctcga agctttccac gcgtgccttg gcgaggacca tggtgttctc ccttcctccg
     8941 cctgtcccgt ccaccctact ccctcagcgc aaggcatcgc ggcagagcaa gccccctggc
     9001 cgcacgcgac caggtgcgtc cgcgatccgt ggagcggtac agttcgcctt cggcggtgac
     9061 gaaggcggcc tgcgagccgt tcgcggcgag ccagaaagtg tcgatgttgt gctcgctggg
     9121 atgaacgggc tcgatccgct cgaagggccc gtcgccgcga aggtcacgtc ggtacacacc
     9181 cgcgtccttg ccggcatggc tgtaggcggc gctcaccagc aacgcatcgc cggcgatcgc
     9241 gaccgcgcgc atgtaggtgt gatcgaagcc gtcgcgatgc tcggtccagg tctcgcctgc
     9301 gtcctcgctg acgtgcagcg cgttccagcc gggtgcgatc acaagatcgt catgcgtgat
     9361 gacttgatgg atgtcggtgt cgatgtcgat ggttggcgtc caggtctcac cgccgtcgcg
     9421 cgaacgcacg atgccgccca cgtgcacgcc ggcgtagatt gtgccgtccg gcgcctcggt
     9481 catcgagcgc acgtcgggcg gcccacccca cggcgtatgc cacgtgtcgc gatcttgaac
     9541 ctggtcgaag gattcgacgc gctgcagcga cgatccgtcc agacggtaca gccgcgcgtc
     9601 ggccgcgccg acgaagagcc cgtggcggct cggcagcaga cagttcgcct cgatgccgtc
     9661 cagcggcgcc acggcgcgca gcgcatcggg ctcaccttgc aggatctgct cggtcgtcag
     9721 aacccacgca ccgtcttcgc catcgatgac gtgcgtcacg tcaccctcga gcagcgccgt
     9781 gccgtcgctc gtcaccagcc cgttcttcgt tccgatgagg atcatcgtca tcactcctcg
     9841 ccgtcgttgc cgtccactct gccacgcgac gcaacgtgaa acacggcatt cgcgataatc
     9901 ttctctcatg gtttccgaat cgcgcatcga tcgggtaacc ggagcggcct tcgtcggaat
     9961 ggtcgcgggc atcgcgggca acgtcgtcgc ggtcaagtac gtcgcgcgcg ccggggatca
    10021 ggatccgatg tgggcagcgg catcgcgctt cgcgctcgcg accgcgatct ttctcgtcat
    10081 cgcgatcgcc gttcgcgcgg cgttcccggt cggacgcgcg ctggccggcg cgttgctgta
    10141 cggctcgctg accttcggcg gcttcttcgt cttcgcctac ttgggtcttc aaagggcgcc
    10201 ggtcggcatc gccagcgtcg tgctcgccac cgggccgttg ctgacgttcg cattcgcgct
    10261 ggcgcaccgg caagagcggt ttcgttggga cagcttgatc ggcggcgccg cggccgtcgc
    10321 cgggactgcg ctggtattcg tgtccggttt ggatcaaggc gtgccgggct ggtcgttgct
    10381 ggcgatcctg gcggcgtcgg cgtgcgccgc ggaaggcacg gtgatcgtca aggcgttccc
    10441 gcccatgcat ccggtgatgc gcaacgccat cgggaccggc gtgggtaccg cgatcctggc
    10501 gctgatgatt ccgttgttcg gtgaatcgat cgcggtcccg tccgagccgc agacatgggt
    10561 ggcgcaggcg tacctgatca cgttcggaac agtcggcgtc ttcggcctat atctgttcct
    10621 gttgagccgg tggacggcaa gcgcggtctc ctacgagttc gtgctcgccc cgctggc
//
LOCUS       DATLFO010000383        15826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_148518, whole genome shotgun sequence.
ACCESSION   DATLFO010000383 DATLFO010000000
VERSION     DATLFO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148518"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VM841_15695"
     CDS             complement(<1..272)
                     /locus_tag="VM841_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91664.1"
                     /translation="MPWCHTCRVEYHAPETACSECGSPLIEATPPERRMAGPTPGTTM
                     RVVATLPAEDALLASGRLDEGGIPASLRPSDGGSIGDATFTFDVLVA"
     gene            612..929
                     /locus_tag="VM841_15700"
     CDS             612..929
                     /locus_tag="VM841_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HVL91665.1"
                     /translation="MSEQTLPTVEEVREILRAVIDPEIGLPIVDLGLVYEIEVHEGGK
                     VRVVYTLTSLGCPVGPMIESNVRQLLESIPGVDEVELEMTFRPPWTPDMMDEEAKAAL
                     GYF"
     gene            complement(976..1290)
                     /locus_tag="VM841_15705"
     CDS             complement(976..1290)
                     /locus_tag="VM841_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91666.1"
                     /translation="MAIVGAAAEAAALAAQAAARGIKPPVRLSGVDARAWMILASGGP
                     ARAGDALMLDEYGDLWWGYAHGPHQARVTRACWPEEAGLYVAGIRSMMELVLGAPAPA
                     AN"
     gene            complement(1631..2047)
                     /locus_tag="VM841_15710"
     CDS             complement(1631..2047)
                     /locus_tag="VM841_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91667.1"
                     /translation="MRRAVLFGALTLALASAAMPARSIPAAGQMELSLSWSPGRPPVG
                     VTTTFTATASDNDGAVRSARLCYGDGTPCDEEVRLLTTAQLVNACVFGDNWGPRQWSH
                     KFTKSGAYPVTLTVTTRGCPGFPDETRSLTTTVTVG"
     gene            complement(2144..2629)
                     /locus_tag="VM841_15715"
     CDS             complement(2144..2629)
                     /locus_tag="VM841_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91668.1"
                     /translation="MKIRFLLTALAATLAMAGPPASAIVPGGPDGSMVWTPGQPTADR
                     DVTFGITARDSDGPIISVRVDFGDGTHANLAVPPRSTLRDASACAFGETFSGSVKHRY
                     ASPGTYQAKLTIVSGSCPLTSALQDATRVKGYELRVVTATAGPADGASAPLGGIANAR
                     G"
     gene            2959..4365
                     /gene="sufB"
                     /locus_tag="VM841_15720"
     CDS             2959..4365
                     /gene="sufB"
                     /locus_tag="VM841_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HVL91669.1"
                     /translation="MATKPADLGLGQGYALGWHDPSTSVNEPKKGLSAAVVEEISKLK
                     GEPEWMTKKRLKAYKHFVDRPMPTWGGDLGEIDFDDIYYYIKPLDHQVKSWDDLPEDI
                     KNTYVKLGIPEAEREFLGGVTAQYESEVVYHQVKKELEDIGVYFSDMDTALREKPELV
                     KEYFGTVIPANDNKFSALNTAVWSGGSFIYVPPNTHVDIPLQAYFRINRENMGQFERT
                     LIIVDEGAYVHYVEGCSAPVYTTDSLHSAVVEIIVKKGARCRYTTIQNWSPNVYNLVT
                     KRAVAYDNAVMEWVDGNIGSKLTMKYPSIYLMGEGARGEVLSVAFAGEGQHQDAGGKV
                     IHAAPNTSSSIISKSVSAHGGRTSYRGLVRVEPDADHAKSFVRCDALILDPESRSDTY
                     PYMEIEDETASTGHEATVSKVGEDQLFYLMSRGIPEAEAMGMIVNGFIEPITKELPME
                     YAIELTRLIQLQMEGSVG"
     gene            4358..5689
                     /gene="sufD"
                     /locus_tag="VM841_15725"
     CDS             4358..5689
                     /gene="sufD"
                     /locus_tag="VM841_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HVL91670.1"
                     /translation="MASAASFDAAAVKALAQRAGDPGWLADRRLEAFERFEALPWPNP
                     RLEAWRYSTLEGFNLEEFRPGMGDHPAVDEPGKVHADVRAQMTIEGERSGQAVQVDAD
                     VVLASVDAAMRDKGVIVTTLARAATDHEELVREYLGSAGVTASDEKFQALTASFAGAG
                     LFVYVPAGVDVPLPIRSFRYITAAGRAAFARTLIVAEENASVTVVEHLRSPHLGGSAL
                     SAATTEIHAKQAANVSVLTVQDYAPTMWHFATARAVIDRDATVRTLAATLGGKFSRSV
                     VESILQGNGGYSELLGLYFGDSKQHFDNRSLQSHLAPNAKSDLYYKGALKGHSTSVYS
                     GLISIAKEAQGTDAWQGNRNLILSEHSKADSIPYLEIEANDVRCAHGASVGPPDEDVL
                     FYLNSRGLDSAAAERLVVKGFFQEVLDRVRVKEVRELLEAAIEAELALEDA"
     gene            5686..5994
                     /locus_tag="VM841_15730"
     CDS             5686..5994
                     /locus_tag="VM841_15730"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HVL91671.1"
                     /translation="MSRVKVCAKGELPDDGAVRVTLDGIPVAVFHHDGEYYALADTCS
                     HEEASLSEGLIDDGEVECPKHGALFSLETGKPLTLPATRPVETYAVVIEDGQIYVENK
                     "
     gene            6048..6779
                     /gene="sufC"
                     /locus_tag="VM841_15735"
     CDS             6048..6779
                     /gene="sufC"
                     /locus_tag="VM841_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVL91672.1"
                     /translation="MLVVEDLHAGIDGRDILKGISLTIRRGEIHAIMGPNGSGKSTLA
                     YLLMGRPGYEITAGTVTFKGEEILELAPDERAKRGLFLAFQYPQEIPGVSVVNFLRTA
                     RNAIHEEMNPMQFMKFLKEKMASLEMDESLVRRYVNEGFSGGEKKRNEILQMAVLEPD
                     LGVLDETDSGLDIDALREVSAGINAVASPQRSMLLITHYQRILNYVKPHVVHVLINGK
                     IVRSGGFELAEELEKSGYDSFRDEG"
     gene            6798..8048
                     /locus_tag="VM841_15740"
     CDS             6798..8048
                     /locus_tag="VM841_15740"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HVL91673.1"
                     /translation="MFDAHVIRKDFPILEKRVHGDKPLVYLDSAATSQHPRQVINTIS
                     DFYEHSNANVHRGVYALGEEATQRYEGARDKVAAFIGAPGPQGLVFVRSATEALNTVA
                     GGWGRKFLKPGDEVLISEMEHHSNLVPWQMIAGATGAKLVPLPVNIETGLLDLDALPA
                     LLTERTKLVCVTQMSNVLGTINPIRQIADAAHAAGALVLVDGAQGAPHIPTDVVAMDA
                     DFYALSGHKMLGPTGSGALWAKPEHLEAMDPFLGGGEMIREVWFDRSTYNEIPYKFEA
                     GTPPIAPAVGLGAACDYLTDLGMDAVHEHEVELTRYAIDRLDDLGEVTIHGPRDMSVK
                     GGVVSFWYGDIHPHDLGTIVDTFGVCIRAGHHCAQPLMRRLGVPATARASFNVYNTTE
                     DIDALIDALTHAKEIFAGGGGLPF"
     gene            8079..8507
                     /locus_tag="VM841_15745"
     CDS             8079..8507
                     /locus_tag="VM841_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HVL91674.1"
                     /translation="MSLDDLYREVILDHYRNPRNKHELEGSTVDLTHNNPLCGDEITI
                     HAVVRDGVVADVAFEGQGCSISQSSASMLTDLVKGKPLADVEKLILDFRAMMAGKPVE
                     DEEALGDLVALKGVTKYPVRIKCAVLAWDTLQEGVQQTEP"
     gene            8754..9785
                     /locus_tag="VM841_15750"
     CDS             8754..9785
                     /locus_tag="VM841_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVL91675.1"
                     /translation="MPPELDQTYRRTFARLKGLFRARGCTPEEAADLAQEAAMRAYVH
                     VRRWGVTGDGLDPLINRIARNLLIDRHRRVAPHLVPLDSAEDVGDPAADPTEEVIRRQ
                     RSHEVRSAVSELPDRHQTALLYSLGGMTPAEVGDKLGIGRNAADALLHRARRSLRDRL
                     RHVGEGAFGVVFWVKIRVRDAAARAGFGVPVEAAGAATGSVAAAVAIVAAMSVVAPLS
                     GGSEGRLSASPVVSVAATEPVSAGGGRVAPMGATVPGEQPEDGVTRFGIPGGPTVEKG
                     DRRIRGNADIDNPAAPDQKLLGVGPDVIHQKNEASAAGTAFDSACKGRETTCNAIYQD
                     PRGDDGEDR"
     gene            9782..9964
                     /locus_tag="VM841_15755"
     CDS             9782..9964
                     /locus_tag="VM841_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91676.1"
                     /translation="MKRLLALVALAAAVLGVGGSPARADSICFRSGLVILGEPWLSHQ
                     QCVPCPTVDCFAPRNS"
     gene            complement(10273..11415)
                     /gene="nadA"
                     /locus_tag="VM841_15760"
     CDS             complement(10273..11415)
                     /gene="nadA"
                     /locus_tag="VM841_15760"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVL91677.1"
                     /translation="MATKPLLPLVLDRAPSPATSELGTECTDLPPASDPALVARAAAA
                     KQALDDQVVILGHHYQKQEVIQFADARGDSYKLAKFAAEAGAPYVVFLGVHFMAESAD
                     ILTGDDQQVILPDLAAGCSMADMAEIGQVEEAWAAIEAVSAGSTVPLTYMNSSAAIKA
                     LTGREGGAICTSSNAAAAMRWAFERKDKILFLPDQHLGRNTAVRALGLSMDDCLVWDP
                     YRDMGGLTDEQIESAKVLLWKGHCSVHQRFQPEHVAAVRERVPGVRVIVHPECRYEVV
                     EAADDVGSTEHIISTIAAAEPGSKWAVGTELNLVTRIASEHPDKTVVFLDRTVCFCAT
                     MNRIDLPHLVWALENLTQGRVVNRVHVEPETAQWARVALQRMLEIS"
     gene            11478..12410
                     /locus_tag="VM841_15765"
     CDS             11478..12410
                     /locus_tag="VM841_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HVL91678.1"
                     /translation="MDLAGIPYLREIDRPIERGPRADPNFPHASEAIADKVVDPVAIM
                     LGVPYSGGSLSGAKCDQTPAAVRGALPRFSAYSTDYGVALEVASILDAGNVDAKGDVE
                     DVERRIEACVRALSSAGVPLAVVGGDNSITAGAAAGADADALITFDAHHDVRDGATNG
                     SPVRRLVEAGLPGERIIQIGIHGFANSRFYTERAAEYGIRWVPATAVRDRGMRAVLEE
                     AIGALAAAGASRIWCDFDIDVLDRAFSPGTAASLPGGLWPQDLEQAAFIAGADERVRG
                     IDITEVDPSTDLAGMTVRVACAVLLSYLAGVESR"
     gene            12407..13930
                     /gene="hutH"
                     /locus_tag="VM841_15770"
     CDS             12407..13930
                     /gene="hutH"
                     /locus_tag="VM841_15770"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010552151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVL91679.1"
                     /translation="MSVRITGDPLGIADVVAVAREGEHVAVDGAVAERMRPGRALVDA
                     AVEREQIVYGITTGFGALANVRIPREAAVELQHSIVRSHAAGAGEPIDNEIVRGMMLL
                     RARTLAAGHAGARPVLVEAIAALLNAGITPVVPRYGSVGASGDLAPLAHCALALLGEG
                     MVRVRGGAPVPAAEALRDAAIEPVVLEAKEGLALVNGTEGMLSMLSLALHDFDLLTRA
                     ADVACAMTVEALLATDRPFAPALHALRPHPGQGVSAGRLRTMTAGSEIIASHRSVEHA
                     VQDAYSLRCAPQVHGACRDTAAHARAVADRELASEVDNPVVLHEIGAVESAGNFHGEP
                     VAFALDFLAIAACELASISERRTDRMLDPAHSNGLPAFLAPDAGVNSGFMLAQYTQAA
                     LVSDAKRLAVPSSVDTIPTSGTTEDHVSMGFHAGLKLRQSLANARTVLAIEVLCAAQA
                     LDLRAPLKPSPAATAACAVLREQVDPMPSDRELSPQITHAVALLPDLVAAAEAAAGL"
     gene            complement(14089..14616)
                     /locus_tag="VM841_15775"
     CDS             complement(14089..14616)
                     /locus_tag="VM841_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91680.1"
                     /translation="MKAKGITLGAAALLVALVLGACGTSQPAGGADPGGEKPRTTTHT
                     EDNWTIVTPVGWVRTDITKDADAKKAIRYAHADGRYVIVAIDPLGSGYESDAVWRYEV
                     KGNRFEIVSKDDCTGGQGCSIDDQRYDGYVFSKNTGPAPKVAGHEYYFIFGNTKSTTI
                     DADEFETIIESLEAK"
     gene            14734..>15826
                     /gene="hutU"
                     /locus_tag="VM841_15780"
     CDS             14734..>15826
                     /gene="hutU"
                     /locus_tag="VM841_15780"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVL91681.1"
                     /translation="MRGSLACVRTIRAPRGGALTAKGWPQEAALRMLHNNLDPEVAER
                     PEDLVVYGGSGKAARSWEAFDAIVRELTALDDDQTLLIQSGKPVGVFRTHPMAPRVLI
                     SNAMLVPDWADWEHFWELEAAGLTMYGQMTAGSWIYIGTQGILQGTYETFAALAAKRY
                     DGTLRGKVVLTAGLGGMGGAQPLAVTMNEGIAICVEVDPARIERRIEHRYLDEQASSL
                     EDAFARAEAARREGRAVSIGVCANAADAFPAVVQRGRAVDVVTDQTSAHDPLNGYVPR
                     GLTVAEAADLRAADPSGYVKRARESMAVHCEAMVALMRGGAEVFDYGNSLRAGAVDGG
                     LDRDRAFSYPGFVPAYLRPLFCEGMGPFRW"
BASE COUNT         2518 a         5359 c         5455 g         2494 t
ORIGIN      
        1 gccaccagca cgtcgaaggt gaaggtcgca tcgccgatcg agccgccgtc cgagggccgc
       61 agcgatgcgg ggatcccgcc ctcgtcgagc cgtcccgacg cgagcagcgc gtcttcggcg
      121 ggaagcgtgg cgaccacgcg catcgtggtg cccggcgtgg gcccggccat gcggcgctcg
      181 ggcggagtgg cttcgatgag cggcgagccg cactcggagc aggccgtctc aggcgcgtgg
      241 tactcgaccc ggcaggtgtg acaccagggc acggcggcgg ctcctttcga tgcctcggat
      301 cgactcgtca actttcttga cggtgcgtcg ttcttcgcct cagaccgact cgtcaacttt
      361 cttgacggtg cggtcgtcct tcgcctcaga ccgactcgtc aactttcttg acggtgcggt
      421 tgtccttctt gacggtgcgg ttgtccttct tgacggtggg ttgtccttga cggtgggttg
      481 tccttgacgg tgcggttgcc cttcgcctcg gatcaatgcg tcaactttct tgacgagtcc
      541 cgtggacagg gaatggtagc gcgcgccggc cgcccggcgc accggcccga attcctcgat
      601 accatggacc tatgagcgaa caaacgcttc ccacggtcga ggaggtccgc gagatcctcc
      661 gggccgtgat cgatcccgag atcggtcttc ccatcgtgga tctggggctg gtctacgaga
      721 tcgaggtgca cgaggggggc aaggtgcgcg tggtttacac gctcacctcg ctcggatgcc
      781 ctgtcggacc gatgatcgag tccaacgtcc gccagctgct ggagtcgatc cccggtgtcg
      841 acgaggtgga gctcgagatg acgttccgcc cgccgtggac gcccgacatg atggacgaag
      901 aggccaaggc ggccctgggt tacttctagc tccgggctac ttctagctcc gggccacttc
      961 tcgccccggc tccttctagt tggccgccgg cgcgggcgcg ccgagcacca gctccatcat
     1021 cgaccggatg ccggccacgt agagcccggc ttcctcgggc cagcatgcgc gcgtgacgcg
     1081 cgcctggtgc ggcccgtggg catatcccca ccacagatcc ccgtactcat cgagcatcag
     1141 cgcatcgccc gctcgggccg gcccccccga cgcaaggatc atccacgcac gggcatcgac
     1201 gccggaaagc ctgaccggcg gcttgatccc ccgcgccgcg gcttgcgccg ccagcgctgc
     1261 ggcttctgca gccgccccca cgatcgccat tgccgaaacc tctcctctcc ggcgcccgct
     1321 catctaacgg accagcagcc gaaagcgtta cgcgatcggg cgtatcagga ggaatcgggc
     1381 aagcaaccgc gcatagcgtg cgaggagcga cagggggaag cttcgcggag caacagaacg
     1441 ctgcgcggtc tgccagggga acgattgcga aaggtcgtag ggctcgtggt cgtcatggcc
     1501 gcgttgctgc tcgccggggc aggcgctcgg gcgggccatt gcgcctccgc catcgtcgtt
     1561 ttcagccgca cccaggcgca gaaggatgcc gggctcgggg cgatcaatcc gtgcgcagga
     1621 ggctcccgcg ctatccgacg gtgacggtcg tggtcaagga ccgcgtctcg tcgggaaagc
     1681 ccgggcatcc tcgggtcgtg accgtgagcg tcactggata ggcgccgctt ttcgtaaact
     1741 tgtggctcca ctgacgagga ccccagttgt cgccgaacac gcacgcgttc acgagctggg
     1801 cggtggtgag cagccgtacc tcctcgtcgc acggcgttcc gtccccgtag cagagcctgg
     1861 ccgaacgcac ggctccgtcg ttgtcggagg ctgtcgcggt gaaggtagtg gtcacgccga
     1921 cggggggccg ccccgggctc cagctgagcg acaattccat ctggccggcg gccgggatcg
     1981 agcgcgcggg catggcggcg ctcgcgaggg cgagcgtcag cgcaccgaaa aggactgccc
     2041 gtctcacata ggccgtattc ggcccgtcac ggccgatccc tcgcgattcg gaagcggagc
     2101 cgcggcaggg agttccgccg tggccggatt gccccgaagc gaactagccg cgggcgttgg
     2161 cgatgccgcc gagcggcgcc gacgcgccgt ccgccggtcc ggcggtcgcc gtgaccacgc
     2221 gaagctcgta gcccttgacg cgggttgcgt cctgcagcgc gctggtcagc gggcacgagc
     2281 ccgacacgat ggtcagcttc gcctggtagg tgcccgggct ggcgtagcga tgcttgaccg
     2341 agcccgagaa ggtctcgccg aaggcgcacg cgctcgcgtc ccgcagcgtg gagcgcggcg
     2401 gcacggcaag gttggcgtgc gtgccgtccc cgaaatccac cctgaccgag atgatcggcc
     2461 cgtcggaatc gcgggcggtg atcccgaagg tcacgtcacg atcggcggtg ggctggccgg
     2521 gcgtccagac catcgagccg tcggggcctc ccggaacgat ggccgaggcc ggcggacccg
     2581 ccatcgccag ggtcgcggca agggcggtga gcaggaacct gatcttcatc acgctcccgg
     2641 gttcgataga gacctcggcc cctccttccg ggcccgcgcc cgatacgcga acctctgcag
     2701 caacgccccg aaggatcccc cggcgctttg cccccgacgc cggaactgcc ccatccggat
     2761 caaggaggcc gggagatcgg cgcgggggcc tgagccgctc ggccgcgtcc cgcggataga
     2821 ggagcgacca ccatgccggc acccggaatc ggacctcatt gcgggaacaa aatccctggt
     2881 agggggagtt tttcctaggg taatcagact gctaaagtag gaattcgatt tagccggagc
     2941 gggagagagg ttttcagcat ggcaaccaag cctgcggatc tgggactggg acagggatat
     3001 gccctcggtt ggcacgaccc ttccacgtcc gtcaacgaac ccaagaaggg cttgtccgcc
     3061 gccgtcgtcg aggagatctc gaagctcaag ggcgagccgg agtggatgac gaagaagcgg
     3121 ctcaaggcct acaagcactt cgtggatcgt cccatgccga cctggggcgg cgacctcggc
     3181 gagatcgact tcgacgacat ctactactac atcaagccgc tcgaccatca ggtgaagtcc
     3241 tgggacgatc tgccggagga catcaagaac acgtacgtga agctgggcat ccccgaggcc
     3301 gagcgtgagt tcctcggcgg cgtgaccgct caatacgagt ccgaggtcgt ctatcaccag
     3361 gtcaagaaag agctcgagga catcggcgtc tacttcagcg acatggacac ggcgctgcgc
     3421 gagaagcccg agctggtcaa ggagtacttc ggcaccgtca tcccggccaa tgacaacaag
     3481 ttctcggcgc tcaacaccgc ggtgtggtcc ggcggatcct tcatctacgt cccgccgaac
     3541 acgcacgtgg acatcccgct ccaggcctac ttccgcatca accgcgagaa catgggccag
     3601 ttcgagcgca cgctgatcat cgtggacgaa ggcgcctacg ttcactacgt cgaaggctgc
     3661 tccgcgccgg tctacacgac cgactcgctg cactcggcag tggtcgagat catcgtaaag
     3721 aagggcgccc gctgccgcta caccacgatc cagaactggt cgccgaacgt ctacaacctg
     3781 gtgaccaagc gcgccgtcgc ctacgacaac gcggtcatgg agtgggtgga cggaaacatc
     3841 ggatccaagc tcacgatgaa gtacccgtcg atctacctga tgggcgaagg cgcccgcggc
     3901 gaggtcctgt cggtcgcctt cgccggcgaa ggccagcacc aggacgcggg tggcaaggtc
     3961 atccacgccg ctccgaacac ctcgtcgtcg atcatctcga agtcggtctc ggcgcacggc
     4021 ggccgcacgt cctaccgcgg cctggttcgc gtcgagcccg acgccgacca cgcgaagtcg
     4081 ttcgtgcgct gcgacgcgtt gatcctggat cccgagagcc gcagcgacac ctatccgtac
     4141 atggagatcg aggacgagac ggcctcgacc ggtcacgagg ccacagtgtc caaggtcggc
     4201 gaagatcagc tcttctatct gatgagccgc ggcatccccg aagcggaagc gatgggcatg
     4261 atcgtcaacg gcttcatcga gccgatcacc aaagagctcc cgatggagta cgcgatcgag
     4321 ctcacgcgcc tgatccagct tcaaatggaa ggctccgttg gctagcgcgg cctctttcga
     4381 cgcggcggca gtcaaggcac tcgcccagcg ggcgggggat cccggctggc tggccgaccg
     4441 gcggctcgag gcgttcgagc gcttcgaggc gcttccgtgg ccgaatcccc ggctcgaggc
     4501 gtggcgctat tcgacgctcg aaggcttcaa cctcgaggag ttccgcccgg ggatgggcga
     4561 ccatccggcc gtggacgagc ccggcaaggt tcatgccgac gtgcgcgcgc agatgaccat
     4621 cgaaggcgag cgcagcggcc aggccgtcca ggtggacgcg gacgtggtgc tcgcttcggt
     4681 cgatgcggcg atgcgcgaca agggcgtcat cgtcaccacg ctcgcccggg cggcgaccga
     4741 ccacgaagag ctggtgcgcg agtatctcgg ctcggcgggc gtgaccgcct ccgacgagaa
     4801 gttccaggcg ctgaccgcct cgttcgcggg cgcgggcctg ttcgtctatg tgccggccgg
     4861 ggtggacgtg ccgcttccga tccgctcgtt ccgctacatc accgccgcgg gccgcgccgc
     4921 gttcgcgcgc acgctgatcg tcgccgagga aaacgcttcg gtcaccgtcg tcgagcatct
     4981 gcgctcgccg catctgggcg gaagcgcgct gtcggcggcc acgaccgaga tccatgccaa
     5041 gcaggcggcg aacgtgtcgg tgctgacggt gcaggactac gcgccgacga tgtggcactt
     5101 cgcgacggcg cgcgcggtga tcgaccggga cgccacggtg cgcacgctcg ccgcgacgct
     5161 cggcggaaag ttctcgcgca gcgtcgtcga gtcgatcctg caaggaaacg gcggctacag
     5221 tgagctgctc ggcctgtact tcggcgactc caagcagcac ttcgacaacc ggtcgttgca
     5281 gtcgcatctg gcgcccaacg ccaagagcga cctgtactac aagggcgcgc tcaagggcca
     5341 ctcgacctcg gtctacagcg gcttgatctc gatcgcgaag gaggcacaag gcaccgacgc
     5401 gtggcagggc aaccgcaacc tgatcctgtc cgagcactcg aaggccgact cgatcccgta
     5461 tctggagatc gaagccaacg atgtgcggtg cgcgcacggc gcgagcgtcg gcccgcccga
     5521 cgaggacgtg ctgttctacc tcaacagccg aggactggat tccgcggcgg ccgagcgtct
     5581 ggtcgtgaag ggcttcttcc aagaggtgct cgaccgcgtg cgcgtgaaag aggtccgcga
     5641 gctcctggaa gccgcgatcg aggcggagct cgcgctggag gacgcatgag ccgcgtgaag
     5701 gtgtgcgcga aaggcgagct gcccgacgac ggagccgttc gcgtgacgct cgacggcatt
     5761 cccgtcgcgg tgttccacca cgacggcgaa tactacgcgc tggccgacac ctgctcgcac
     5821 gaagaagcgt cgctgtcgga aggcctgatc gacgacggtg aagtcgaatg tcccaagcac
     5881 ggcgcgctgt tcagcctgga gaccggcaag ccgctgacgc tgccggcgac ccggccggtc
     5941 gaaacctacg ccgtcgtgat cgaagacggt cagatctacg tggagaacaa gtgatgagcg
     6001 agacccagat gatcgaaaag aacgcagacg tcaagcccgg cgaggagctg ctggtcgtcg
     6061 aagacctgca cgccggcatc gacggccgcg acatcctgaa gggcatctcg ctgacgatcc
     6121 gacgcggcga gatccacgcg atcatgggcc cgaacggctc gggcaagtcc acgctcgcgt
     6181 acctgctgat gggccggccc ggctacgaga tcaccgccgg cacggtcacc ttcaagggcg
     6241 aggagatcct cgagctcgcg cccgacgagc gcgccaagcg tggcctgttc ctggccttcc
     6301 agtacccgca agagatcccg ggcgtatcgg tcgtgaactt ccttcgcacc gcgcgcaacg
     6361 ccatccacga ggaaatgaac ccgatgcagt tcatgaagtt cctcaaggaa aagatggcgt
     6421 cgctggagat ggacgagtcg ctggtgcgcc gctacgtcaa cgaaggcttc tcgggcggcg
     6481 agaagaagcg aaacgagatc ctccagatgg ccgttctgga acccgacctg ggcgttctgg
     6541 acgagaccga ttccgggttg gacatcgacg cgttgcgcga ggtctcggcc gggatcaacg
     6601 cggtcgcctc gccccagcgc tcgatgctgc tgatcacgca ctaccagcgc atcctcaact
     6661 acgtgaagcc gcacgtggtg catgtgctga tcaacggcaa gatcgtccgc agcggcggct
     6721 tcgagctcgc ggaagagctc gagaagagcg gatacgattc cttccgggac gaaggctaaa
     6781 ggcgcaggag gcccgtcatg ttcgacgcgc acgtgattcg caaggacttt ccgatcctgg
     6841 aaaagcgcgt gcacggcgac aagccgctgg tctacctcga ctcggcagcc acctcgcagc
     6901 acccgcggca ggtcatcaac acgatctccg acttctacga gcactccaac gccaacgttc
     6961 accgcggcgt gtacgcattg ggggaagaag cgacgcagcg ctacgaaggc gcgcgcgaca
     7021 aggtcgccgc gttcatcggc gcgccgggcc cccaggggct ggtcttcgtg cgcagcgcga
     7081 ccgaggcgct caacaccgtc gcgggcggtt ggggccgcaa gttcttgaag cccggcgacg
     7141 aagtgctcat cagcgagatg gagcatcaca gcaacctggt gccgtggcag atgatcgcgg
     7201 gcgccaccgg cgcgaagctg gtgccgttgc cggtgaacat cgagaccggc ctgctggacc
     7261 ttgacgcgct gccggcgctt ctgaccgagc gtacgaagct ggtgtgcgtc acccagatgt
     7321 cgaatgtgct cggcacgatc aacccgattc ggcagatcgc cgacgcggct cacgcggccg
     7381 gcgctctcgt gctggtcgac ggggcgcagg gtgcgccgca cattccgacc gacgtggtgg
     7441 cgatggacgc ggatttctac gcgttgtcgg gccacaagat gctcggcccg accggctcgg
     7501 gcgcgctgtg ggccaagccc gagcatctgg aagcgatgga tccgttcctg ggcggcggcg
     7561 agatgatccg cgaagtctgg ttcgaccgct cgacctacaa cgagatcccc tacaagttcg
     7621 aagccgggac gccgccgatc gcgccggccg tgggtctggg cgcggcatgc gactacctga
     7681 ccgatctcgg catggacgcc gtgcacgagc acgaagtcga gctgacccgt tacgcgatcg
     7741 accgcctcga cgatctcggc gaggtcacga tccacggtcc gcgcgatatg agcgtcaaag
     7801 gcggcgtggt ctcgttctgg tacggcgaca tccacccgca cgatctcggc acgatcgtgg
     7861 acacgttcgg cgtgtgcatc cgtgccggtc atcactgcgc gcagccgctg atgcggcgtc
     7921 tgggcgttcc cgcgaccgcg cgtgcgtcgt tcaacgtcta caacaccacc gaagacatcg
     7981 acgcgctcat cgacgcgctc acccacgcga aggagatctt cgcgggcggg ggagggctgc
     8041 cgttctgatg agccgccacc gggtatcctg acgccgccat gtcactcgac gatctgtacc
     8101 gcgaggtcat cctcgaccac taccgcaatc cgcggaacaa gcacgagctg gaaggctcga
     8161 ccgtcgatct gacgcacaac aacccgttgt gcggcgacga gatcaccatc cacgcggtgg
     8221 tgcgcgacgg ggtcgtcgcc gacgtcgcct tcgaaggcca gggctgctcg atttcgcagt
     8281 ccagcgcgtc gatgctgacc gatctggtga aaggcaagcc cctcgccgac gtcgagaagc
     8341 tgatcctcga cttccgggcc atgatggccg gcaagccggt cgaggacgag gaggcgctcg
     8401 gtgatctggt cgcgctcaag ggcgtcacga agtatccggt gcggatcaaa tgcgccgtcc
     8461 tggcctggga cacgctgcaa gaaggcgtcc agcagaccga gccctaggcg ctcccgggtc
     8521 acgaccacct gtctcgaacg ttcgttctcg cagccgttcc ggaaaaaccg gcaaaggcag
     8581 gcagggctag caaaagccgg aagggacgag ggcgcccggc gcgaataatc accgaaggcc
     8641 ggggttccaa attgggtgca ccggccgtag actcacaacg gccgcaaagg ggatggaaat
     8701 cccggagggg gtgaacggcc ctgtcgatcg aagcacgctt tctcgagccc gacattccgc
     8761 ccgagctcga tcagacgtat cggcgcacgt tcgcgcggct caaaggcctg ttccgggccc
     8821 ggggctgcac gcccgaggaa gccgccgacc tcgcccagga agccgccatg cgcgcctacg
     8881 tgcacgtgcg gcgctggggc gtcaccgggg acgggctcga cccgctgatc aaccgtatcg
     8941 cccgcaatct tctgatcgac cgtcatcgcc gcgtcgcgcc gcatctggtg ccgctcgact
     9001 cggctgagga cgtcggcgat ccggcggccg accccaccga ggaggtcatc cgccgccagc
     9061 gaagccacga ggttcgcagc gccgtgagcg agcttcccga tcgccaccag actgcgctgc
     9121 tgtactcgct gggcggcatg acgcccgcgg aggtcggcga caagctcggc atcggccgca
     9181 acgccgccga cgcgctgctg catcgcgcgc gccgctcgct tcgcgaccgg ctccgccacg
     9241 tcggcgaagg agccttcggc gtggtctttt gggtcaagat ccgcgtccgg gacgcggccg
     9301 ctcgcgccgg tttcggcgtg ccggtcgaag cggccggcgc cgcgacggga tccgtcgccg
     9361 ccgccgtggc gatcgtggcc gcgatgagcg tggtcgcacc cttgtccggc ggatccgagg
     9421 gccggctgtc cgcgagcccg gtcgtgtcgg tcgcggcgac cgagccggtc tcggccggcg
     9481 ggggccgcgt cgctcccatg ggcgcgacgg tccccggcga gcagcccgaa gacggcgtga
     9541 cccgcttcgg gatccccggc ggccccaccg tcgagaaggg tgaccggcgg atccgcggca
     9601 acgccgacat cgacaatccc gccgcgcccg accagaagct gctcggcgtc ggtcccgatg
     9661 tgatccatca gaagaacgaa gcgtcggccg ccggcaccgc gttcgactcg gcctgcaagg
     9721 gacgagagac gacctgcaac gcgatctacc aggacccgcg cggggacgac ggggaggacc
     9781 gatgaagcgt ctgctcgcgc tggtggcgct cgcggctgcc gtgctcgggg taggcggctc
     9841 gcccgcgcgt gccgactcga tctgcttccg cagcggtctg gtgatcctcg gtgagccctg
     9901 gctctcgcac cagcagtgcg tgccctgccc gaccgtcgac tgcttcgctc cgcgcaacag
     9961 ctagcgcgca aacccgaccg taagacggcc ctttccgggt cgtagttctt agtgtgcggg
    10021 ccgggaacgc caacagcagg ctcgcagggc cggtcgctca ccctgggagc gggcggctca
    10081 agacctggca gtgaggggat gtttcgccgg cgcccgccgg cgagcccgag ccaacctcac
    10141 cgcggtctcc tcaggggggt gggaaccctg gcccgccccc ctttctcttt gcagcgtccg
    10201 gcctcggggg agagccggcc gaacggtcga aggggcccga tcgcgggccc tttcggcacg
    10261 ctcgtgggcg ggctaggaga tctcgagcat ccgctggagc gccacgcgtg cccactgcgc
    10321 cgtctccggc tcgacgtgta cacggttgac gacgcgccct tgtgtgaggt tttcgagcgc
    10381 ccagaccaga tgcggcagat cgatgcggtt catcgtcgcg cagaagcaca cggtgcggtc
    10441 caggaacacc acggtcttat cgggatgctc ggacgcgatg cgcgtcacca ggttcagctc
    10501 ggtgccgacc gcccacttgg atcccggctc ggcggcggcg atcgtggaga tgatgtgctc
    10561 ggtcgagccg acgtcgtcgg cggcctcgac gacctcgtag cggcactccg gatgcacgat
    10621 gacccgcacg ccgggcaccc gctcgcgcac ggcggcgacg tgctccggct ggaatcgctg
    10681 gtgcacgctg caatggccct tccacagcag caccttcgcc gattcgatct gctcgtcggt
    10741 gagcccgccc atgtcgcgat acggatccca cacgaggcaa tcgtccatcg acaggcccag
    10801 cgcgcgcaca gccgtgttgc ggcccagatg ctggtcggga aggaacagga tcttgtcttt
    10861 gcgctcgaac gcccagcgca tggcggcggc cgcgttcgac gaggtgcaga tcgcgccgcc
    10921 ctcgcgcccg gtcagcgcct tgatcgcggc cgaggagttc atgtaggtca gcggaaccgt
    10981 gctgcccgcg gagaccgcct cgatggcggc ccaggcttct tcgacctggc cgatctcggc
    11041 catgtcggcc atcgagcagc cggccgcgag gtcgggaagg atcacctgct ggtcgtcgcc
    11101 ggtcaggatg tcggcggact cggccatgaa gtgcacgccc aggaagacca cgtatggtgc
    11161 gccggcttcg gcggcgaact tcgcgagctt gtagctgtcg ccgcgcgcgt cggcgaactg
    11221 gatgacctct tgcttttggt agtggtggcc gaggatcacg acctgatcgt cgagcgcctg
    11281 cttggccgcg gcggcgcgag ccaccagcgc cgggtcggat gcgggcggca ggtcggtgca
    11341 ttcggtgccg agctcgctcg tggcggggct gggcgcgcgg tccaggacca gcggcagcag
    11401 aggtttcgtg gccatgcgtt catcgtagtg ccctcgcagg gcggccgcga atgcgttccc
    11461 ctctatgatt gggcgccgtg gaccttgccg gcatcccgta cctgcgcgag atcgaccgtc
    11521 cgatcgagcg cggccctcgc gcggatccga acttcccgca cgcgtccgag gccattgcgg
    11581 acaaggtcgt cgatccggtc gcgatcatgc tcggcgtgcc ctacagcggc ggctcgctgt
    11641 cgggggccaa atgcgatcaa acgcccgccg ccgtccgcgg agcgctccct cgcttctcgg
    11701 cctactcgac cgactacggc gtggcgctcg aggtcgcctc gatcctggac gccggcaacg
    11761 tcgacgcgaa aggggacgtc gaggacgtcg agcggcgcat cgaggcgtgc gtgcgcgcgc
    11821 tgtcctcggc cggtgtcccg ctcgccgtcg tcggcggcga caactcgatc accgccggcg
    11881 cggcggccgg cgccgacgcc gacgccctga tcacgttcga cgcgcatcac gatgtgcggg
    11941 acggcgcgac gaacggctca cccgtgcgtc ggctggtcga agcagggctg cccggtgagc
    12001 gcatcatcca gatcggcatc cacggcttcg ccaactcacg cttctacacc gaacgggccg
    12061 ccgagtacgg gatccgctgg gtcccggcga ccgccgtccg ggatcgcggg atgcgcgccg
    12121 tgctcgagga agccatcgga gcgctcgcgg cagcgggggc atcgcgcatc tggtgcgact
    12181 tcgatatcga cgtgctcgac cgtgcgtttt cgcccgggac ggccgcctcg cttccgggcg
    12241 ggctgtggcc gcaggatttg gagcaggccg cgttcatcgc cggcgccgac gaacgggtac
    12301 gcggcatcga catcaccgag gtggacccgt ccaccgacct cgcgggcatg accgtgcggg
    12361 tcgcgtgcgc cgtgctgctg tcgtaccttg ccggcgtgga gtcccgatga gcgttcgcat
    12421 aacgggcgat ccgttgggga ttgccgacgt cgtggcggtc gcccgcgaag gcgagcacgt
    12481 cgcggtcgat ggggccgtcg ccgagcggat gcggcccggc cgcgcgctgg tggatgccgc
    12541 cgtcgagcgc gagcagatcg tctacggcat caccaccggc ttcggggcgc tcgccaacgt
    12601 gcgcatcccg cgcgaagccg cggtcgagct gcagcactcg atcgtgcgct cgcacgcggc
    12661 cggggcgggc gagccgatcg acaacgagat cgtgcgcggc atgatgctgc tgcgcgcccg
    12721 cacgctcgcg gccgggcacg cgggcgctcg cccggtgctg gtggaggcga tcgcggcgct
    12781 gctcaacgcg ggcatcacgc cggtcgtgcc gcgctacggc tcggtgggag ccagcggcga
    12841 tctggcgccg ctcgcgcatt gcgcactcgc gctgctgggc gaaggcatgg ttcgtgttcg
    12901 cggcggcgcg cccgtgccgg cggccgaagc gctgcgcgat gcggccatcg agccggtcgt
    12961 gctggaggcg aaggaagggc tcgcgctggt caacggcacc gaaggcatgc tatcgatgct
    13021 gtcgctggcc ctgcacgact tcgatctgct cacccgcgcg gccgacgtcg cgtgcgcgat
    13081 gacggtcgag gcgctgctcg cgaccgaccg gccgttcgcg ccggcgcttc atgcgctgcg
    13141 tccgcacccc gggcaaggcg tatctgctgg gcggctgcgg accatgaccg ccgggagcga
    13201 gatcatcgcc tcgcatcgca gcgtcgagca cgccgtgcag gacgcctact cgctgcgctg
    13261 cgcgccgcag gtgcacggcg cctgccgcga cactgccgcc cacgcgcgcg ccgtcgccga
    13321 tcgcgagctt gcctccgagg tcgacaaccc ggtcgtgctg cacgagatcg gcgcggtcga
    13381 gtcggccgga aacttccacg gcgagccggt cgcgttcgcg ttggacttcc tggcgatcgc
    13441 cgcatgcgag ctggcatcga tcagcgaacg gcgcaccgac cggatgctcg acccggcgca
    13501 ctccaacggc ctgccggcgt tcctggcgcc ggacgcgggc gtgaactcgg gcttcatgct
    13561 tgcccagtac acgcaggcgg cgctggtctc ggacgcgaag cggctggcgg tcccgtcgtc
    13621 ggtggacacg atcccgacat cgggcacgac cgaggatcac gtcagcatgg gcttccacgc
    13681 cggcctgaag cttcgccagt ccctggcgaa cgcgcgcacc gtgctcgcga tcgaggtgct
    13741 gtgcgcggcg caggcgctgg atctgcgcgc gccgctgaag ccgtcaccgg ccgcgacggc
    13801 cgcctgcgcg gtgctgcgcg agcaggtcga tccgatgccg tcggaccgcg agctgtcccc
    13861 gcagatcacg cacgccgtcg cgctgttgcc ggatctggtc gcggcggccg aggccgcagc
    13921 cgggctctag gccgcggtct cggccgggaa cgcgaagcgg cgcgccgtcc ggtcaagcct
    13981 gcgtcgtggg ccgattcgtg ttcgatcggt cccggcgggc cggcttcgtg ttcgagccgt
    14041 ccgtccttcg cagcctcgcc cggggcgcgc cgccccgatc gtgctcgcct acttcgcttc
    14101 gagactttcg atgatcgtct cgaactcgtc cgcatcgatt gtcgtgctct tggtgttccc
    14161 gaagatgaag tagtactcgt ggcccgcgac cttcggtgcc ggtccggtgt tcttcgagaa
    14221 cacgtacccg tcgtagcgct ggtcgtcgat gctgcagcct tgaccgcccg tgcagtcgtc
    14281 cttggagacg atctcgaagc ggttgccctt gacctcgtag cgccagaccg cgtcgctttc
    14341 gtagcccgac cccagcgggt cgatggccac gatcacgtag cggccgtcgg catgggcgta
    14401 gcggatcgcc ttcttcgcgt ctgcgtcctt ggtgatgtcg gtgcgaaccc agccgaccgg
    14461 cgtcacgatg gtccagttgt cctcggtgtg ggtcgtcgtc cggggctttt cgcctccggg
    14521 atcggcgccg cccgccggtt gcgacgtgcc gcacgccccc aggaccagcg cgaccagcag
    14581 tgcagccgcc cccagagtga tgcccttcgc tttcatgact cctcctgtcc ttccatcgca
    14641 tctatgacga cgggaccggc cgcggggttc cgctaccttt tgcaagcgat tccgccccgg
    14701 gtccgagcgc ccggcacggc cgggaaattc ccgatgcgcg gtagtctggc ctgcgtgaga
    14761 acgattcggg ctccgcgggg tggcgctctc acggccaagg gctggccgca agaggcggcg
    14821 cttcggatgc tgcacaacaa tctggatccc gaggtcgccg aacggcccga ggatctcgtc
    14881 gtctacggcg gttcgggcaa ggccgcgcga agctgggagg cattcgacgc gatcgtacgc
    14941 gagctgaccg cgctcgacga cgaccaaacg ttgctgatcc aaagcggcaa gcccgtcgga
    15001 gtgttccgga cccatccaat ggcgccgcgc gtgctgatct cgaacgcgat gctggtcccc
    15061 gattgggcgg actgggagca cttctgggag ctcgaggccg ccgggctgac gatgtacggt
    15121 cagatgacgg ccgggtcgtg gatctacatc gggacgcaag ggattctgca aggcacctac
    15181 gagacgttcg cggccctggc ggccaaacgc tacgacggga ccttgcgcgg caaggtggtg
    15241 ctcaccgcgg ggctgggcgg catgggcggc gcgcagccgc tggcggtcac gatgaacgag
    15301 ggcatcgcga tctgcgtcga ggtggatccg gcccggatcg agcgccgcat cgagcatcgc
    15361 tatctggacg agcaggcgtc ctcgttggag gacgcgttcg cgcgggccga ggcggcccgc
    15421 cgcgaggggc gcgcggtctc gatcggcgtg tgcgccaacg cggcggatgc gttcccggcg
    15481 gtggtgcagc gcggccgggc ggtggacgtg gtgaccgacc agaccagcgc gcacgatccg
    15541 ctcaacggct acgtgccccg cggcctgacc gtggctgagg ccgccgatct gcgcgcggcc
    15601 gacccgtccg gctacgtcaa gcgcgcgcgg gaatccatgg ccgtgcactg cgaggcgatg
    15661 gtcgcgctga tgcgcggcgg cgccgaggtc ttcgactacg gcaactccct gcgggccggc
    15721 gcggtcgacg gcggcctcga ccgcgaccgg gccttctcct atcccgggtt cgtgcccgcc
    15781 tacctccggc cgctgttctg cgagggcatg ggcccgttcc ggtggg
//
LOCUS       DATLFO010000384        17105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U13590
            k141_128749, whole genome shotgun sequence.
ACCESSION   DATLFO010000384 DATLFO010000000
VERSION     DATLFO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939472
            Sequence Read Archive: SRR8438988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:34:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,131
            Genes (coding)                    :: 3,113
            CDSs (with protein)               :: 3,113
            Genes (RNA)                       :: 48
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128749"
                     /isolate="SMAG_U13590"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VM841_15785"
     CDS             <1..761
                     /locus_tag="VM841_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVL91682.1"
                     /translation="GPRRGKLVLQLVADEEVLGPHGTMALHQRGLAGADAAIVGEPTD
                     LNVAVAERGMLWIIARTAGVAAHGSRPDLGVNAIEEMARAIPVLRALRTGRSHPLLGE
                     PSLNFGTVEGGSKINIVADACRLAMDRRTLPGETDQGIIDQMQAALKDAGLSVTLEIV
                     HRADPVEVDPGEEIVRRAVEAHEAVRGRPGAIGAMTGATDAHVLAGTAGIPTIIFGPG
                     SLDQAHTTGEHVAITDLIDGARIYAELFDAYLSG"
     gene            complement(758..1546)
                     /locus_tag="VM841_15790"
     CDS             complement(758..1546)
                     /locus_tag="VM841_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVL91683.1"
                     /translation="MDLGLRGRAALITASSKGIGRAAAFALASEGCDVAICARGEDTL
                     RETESELSKLGVRVRATVADVSKPEDCRRVVEGAADEFGRLDILVPIAGGPPPGPFDA
                     FDDDAYKRALELDLMSVVRLCTAAVPHMRAQRWGRIVTVQSVAIKQPLQGLVLSNTAR
                     AGVAGFMKTLANELAAEGITANTVCPGPTLTDRIRTLAEDGARQGGVSFDEVIKSFEA
                     DIPMRRLGKPEEIAATVAFLSGEPAAFLTGVTVQVDGGMTRALL"
     gene            1603..1965
                     /locus_tag="VM841_15795"
     CDS             1603..1965
                     /locus_tag="VM841_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HVL91684.1"
                     /translation="MGKPEKYVYVCTNERPEGHPKGSCVQRGCLDVFQELRDQQGEKA
                     LTHFKVVATGCLEPCLAGPTLYIAPDDVWYGGVTVQDVEQIIDQHLVGGKPVDFLTLT
                     DDDFERSQRAAKLAPPSM"
     gene            2045..3211
                     /locus_tag="VM841_15800"
     CDS             2045..3211
                     /locus_tag="VM841_15800"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HVL91685.1"
                     /translation="MTETVPETTTVERPYGQWKELFHEVVTPNYCTGCAACVMACPRD
                     VLGYDHSKAYKPFNIEDSTPIDDCVHGQRGCDICTKACPRFRVWEVECDEALFGRARR
                     PDEVFGIYKEILLVRASNPDIFKAGQDGGLVSALLIYGLENDLIDGALVSKFSDERAF
                     DVVPGLVTDREGVLATAGSRYTYSANPLAMKEAEALKLKNLALVGMSCQASINGTLTA
                     RNVAKYKRRIALTIGLLCSKSFDYDGLMVGKVQNELGIPLEDVAKVNVKGKFQIWRKS
                     TGEQIDVPLKECHPFTREGCKLCPDFAAEHADISTGGLGQSDGWTLTIVRTERGAEWM
                     RALTDAGWIETRPGEEDPGAIALMGKLAAKQRKRWTAGGDRAAPGVLPPEDGNL"
     gene            3221..3994
                     /locus_tag="VM841_15805"
     CDS             3221..3994
                     /locus_tag="VM841_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL91686.1"
                     /translation="MSDPMVLTYEERGGGPALVLIHGFPLNSSMWIEQLKGLAKVRTV
                     IAVNLRGHGYTQVDSADGFSMDLFADDVAATLDDIGVDKADICGLSMGGYVAMAFWRR
                     HKDKVRSMIFADTKPEADSDEAKAGREKTAAAVREQGIQPLVDGVLPKMFGPNPSEEL
                     VAATRAMFESTPPEVAAADLLAMRDRPDSDLSVIDVPCLWIHGEGDQIMPIDAARTAA
                     GTIPDCTFVAVPDAGHMSPLENPGVVIPAMTEFLQKVGK"
     gene            complement(4070..4390)
                     /gene="fdxA"
                     /locus_tag="VM841_15810"
     CDS             complement(4070..4390)
                     /gene="fdxA"
                     /locus_tag="VM841_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HVL91687.1"
                     /translation="MTYTIAEPCIDVKDKACIEECPVDCIYEGPRMLYIQPDECVDCG
                     ACEPACPVEAIFYEDDVPDMWKQFTPVNKEWFDQTGLGSPGGAAKAGPQGVDHPVVAN
                     WQAG"
     gene            4464..4751
                     /locus_tag="VM841_15815"
     CDS             4464..4751
                     /locus_tag="VM841_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91688.1"
                     /translation="MRYEHTNRLGCGDVGRRVTVRSRTADGFMTDAVGVLEECDDRSF
                     VIRTKRGDLIRVERALVVAAKTIPDAPGRPGSRGATGADDTGADDTGAGPV"
     gene            4854..5492
                     /locus_tag="VM841_15820"
     CDS             4854..5492
                     /locus_tag="VM841_15820"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL91689.1"
                     /translation="MPVRSVAFPSHDGVRLEGRLHLPQTAPRGVAVLCHPPTPADGLM
                     PNVLMPSLTRALDTAGWASLRFNYRGIGRSQGAPASARSELDDVRGAIAWAEDAVATD
                     VTAVVGWSSGATIALWACTGSLSVDVFVGLSPPVSVSGPGIPSLPEPQRLADWTTRTL
                     LVCGSADPACDERDAAELAKMLGGEMELVPGADHAFNGRLAEMSALVTWFLR"
     gene            5540..6178
                     /locus_tag="VM841_15825"
     CDS             5540..6178
                     /locus_tag="VM841_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVL91690.1"
                     /translation="MEARAVGFSSGAGPELEGRLAIPDGPRGVAVLCHAYPPMGGSMS
                     NAILPALQRALYKAQWASLRFNFRGVGRSEGSFDGGRGEIEDVAAALAFVRAEAPGAT
                     VAVAGWSFGSVVGLVAAVRDGDVAAYAGIAPPVSAGSRIELPALPAPEAAGSLRTLFV
                     CGTQDPVSLPADVERLAQRYGGASVVIDGAGHFFDGRHDVLAGTVLRHLESE"
     gene            complement(6204..6566)
                     /locus_tag="VM841_15830"
     CDS             complement(6204..6566)
                     /locus_tag="VM841_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVL91691.1"
                     /translation="MTAILIGIGIGLLAGVLSGLFGVGGGIVMVPALVLLGLTQREAS
                     GTSLAALVAPVAIFGVIEYARRHEIRVPYAIGLGIGLAIGAFFGARLAGKISNVMLMR
                     GFGALMLAVSIRFLFFAR"
     gene            6632..8026
                     /gene="cysS"
                     /locus_tag="VM841_15835"
     CDS             6632..8026
                     /gene="cysS"
                     /locus_tag="VM841_15835"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVL91692.1"
                     /translation="MRLYDTMERAVVEMRPERENTLTMYSCGPTVYRFAHIGNLRTFL
                     MADLICRAYRFHGIEARQIQNITDVGHMTDELFDRGEDKMLVAAGVENKSPQEIASFY
                     TAAFLEDVDAIGIRRAILYPKASDHVPQMIEIIAKLIERGHAYAVDGHVYYDTASFPA
                     YGRLSGNTLDELQTGHRKEIGDPNKRHHSDFVLWMHAGPNRVIKFPSPWGDGFPGWHI
                     ECSAMSIEHLGDRFDIHTGGEDNVFPHHEDEIAQSDGAAGHQVVRHWVHGAHLLSEGR
                     KMAKSAGNFYDLRDLAAMGHAEPLAVRLLLLQSRYRAQMNFTRDALGAAETTLARWRR
                     LVGDWSRGAAAPRSDLESKYDTRFSETIADDLDTTSAVALAAEVVSTPDLAPGAKARL
                     LARWDAFLGLNLQREAGEAAAVPTDIAELLAQRQQARAAKDFAESDRLRGQIRGLGFD
                     VEDTAEGQRTRPIG"
     gene            complement(8082..8585)
                     /locus_tag="VM841_15840"
     CDS             complement(8082..8585)
                     /locus_tag="VM841_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVL91693.1"
                     /translation="MPPVLETARLVFRRFEPGDLDALAALYADPEIRRFFPEGILTRA
                     ETHEEVTFFLNGHPKNQDLGLWAAIERETGAFVGRCGLLPWTIDGVEEVEVAFLIAKD
                     RWGEGLATEAARGLVTYAHERLGLARLICLIMYGNEASVRVAEKAGFRFEREHTDEYG
                     PCMIYAR"
     gene            complement(8592..9041)
                     /locus_tag="VM841_15845"
     CDS             complement(8592..9041)
                     /locus_tag="VM841_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVL91694.1"
                     /translation="MRSATSTIRLSAGQRYGAVDCTDDLRRAIKDAGITNGCAVAYCQ
                     HTTAALVINEWEDGVHHDLARRLDVLVPPDEYYAHDDMDRRTQNIEDEHERPNGRAHL
                     AQMLVGATSHAIPVADGEPMLGRWQRLIFVELDDPRDRDVLFHVFGE"
     gene            complement(9038..10168)
                     /locus_tag="VM841_15850"
     CDS             complement(9038..10168)
                     /locus_tag="VM841_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVL91695.1"
                     /translation="MTRPLQSFTSIRAKLGAAIIAAVVVTIVLLYVVLAIMLPGKASA
                     AAAWHSTGAFLRDAWWLLLLAGGLSGASALLLVRVLARGMTKPLRDMARATQQMSRGD
                     YSQRVHTDSRDEVGRLAEAFNHMAADLEGLERLRRDLLANVSHELKTPIAAIRAHLEN
                     LLEGVEQPDRETLQVMLTQSERLSALVDQVLDLSRLDSGAAAMTFEPVALASVAGQVA
                     AEVAIARRDRSVAVRNLIDPGLPAAQADRARVHQVLFNLLDNAVRLTPAGGEVSLIAD
                     RESKHVKVMVEDRGPGIARENIPLVFERFYRADASRSRSDGGAGLGLAIARSIVEAHG
                     GRIGAEPRSGGGMRFWFTLPAAGPEAETPALAHAMQRNEEEM"
     gene            complement(10165..10854)
                     /locus_tag="VM841_15855"
     CDS             complement(10165..10854)
                     /locus_tag="VM841_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVL91696.1"
                     /translation="MTTASILVVEDEASIAEAVRARLIAEGFDATVVGDGPAAVERCE
                     ADPPDLVVLDLMLPGMDGLEVCKRIQRREPIPVLMLTARTDEADKIAGFAVGADDYLT
                     KPFSMRELIYRVRAILRRAERSRGGTGDLVQRGPLAIDPARRRVTTDGSEVSLTPIEF
                     DILHTLAREPGVVYNREKLMDTVWGYVDATGGRVVDSHVARLRKKLAEANPDDTPVRT
                     VHGVGYAFRDV"
     gene            10952..11314
                     /locus_tag="VM841_15860"
     CDS             10952..11314
                     /locus_tag="VM841_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HVL91697.1"
                     /translation="MPAADKDPGVEPDARFSFANERTFLAWNRTALALIVAGLAIVQV
                     LPEFDLPGGSRIIGIPMIVLGGTISFSSYGRWRANEQALRTRSPLPPSRLPWIVVAGI
                     TAGAVLAIVIEALAGSGG"
     gene            11311..11637
                     /locus_tag="VM841_15865"
     CDS             11311..11637
                     /locus_tag="VM841_15865"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HVL91698.1"
                     /translation="MNEDDDLLSGLAGERTRLAWNRTALALAATGGAILKGLPSIRRP
                     RDTVVGILIVALAGLLWFIGSLRYRTQRDAQRRNVLDTHGLRIVAAGVSAAATIALAI
                     ALLPSR"
     gene            11715..13127
                     /locus_tag="VM841_15870"
     CDS             11715..13127
                     /locus_tag="VM841_15870"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HVL91699.1"
                     /translation="MNPTWTRTATAAGLGIAFDLAAHEQQLGVSMPLLVAVAATAVAV
                     TSPHSRERLLLLGAAVAVAPVAAIRSDEAVTAPILLAIMALFVLAATGPGAGALSLRG
                     FLRRGLGVMRALLRSPLFIGAPIRVAVPAPPRVRRVLRSAVIAAPLVAVFALLFASAD
                     RVFAQALLPDLDVRLDALAGHITLIAMGTWMAGAWWPAEAQTAGAIAARDPAAVRPPR
                     LSPAEWSTALGALTALFTVFVAVQFAFLFGGRARVEVTPGLTYAEYARSGFFQLLAAG
                     GLTAMVLLGAWDHGRRDGRRDERRFLGLAAALTALCLVVLASAALRLALYQQAFGFTI
                     SRLAAWLAIGVTGAALCGLLVAIATRRRRNVLSGALAIGLGALIVWAGISPAAFVARK
                     NIERYHRTGTIDIGYLATLGPEATPALVAFVASAPATVTEPLRYALCSFAPERRGDWR
                     SANLARSRAAAARATVRCPA"
     gene            complement(13132..14175)
                     /locus_tag="VM841_15875"
     CDS             complement(13132..14175)
                     /locus_tag="VM841_15875"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVL91700.1"
                     /translation="MRAILFDDVGLISFGDYPEPSVQDPGDAVIKITTAAICGSDLHL
                     LHGKIPGLRPGSVIGHEFVGIVESVGAGVKRFKAGDRVVGSFMIACGACWACERGDYN
                     LCEDMWVLGYGMFVGDLDGAQAEYVRIPKADLNLHAIDPGMTDEQAIFAGDILSTAGY
                     ITSRAEIQEGDTVAITGAGPVGLFSLMHAMTYNPSRVYVVDQAEDRLKLAESLGATPI
                     DMTKVNPVVALQRETGERGVDVVIEAVGATPALTTALNAVRPGGTVAVIGVYTDLSWD
                     FPLGEVFRRAIRLEFGGSANVQGHWTRSLQLVQQGVIDPTVIITHTLPLDEGLRGYEL
                     FESREALKVVLKP"
     gene            14396..14806
                     /locus_tag="VM841_15880"
     CDS             14396..14806
                     /locus_tag="VM841_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91701.1"
                     /translation="MPSLDFAFLADAAEAEPGRKFYVLGGGIDQIGGQSFPLVHPHMA
                     LVMRWVINPTEMNLRHHLEVRLVDADGQQLAKIEGDIEASGGAPMGREAGVNMVINLT
                     NTRFEKPGDFAFEVLMNNQHFKSLGLRVATVPVA"
     gene            15038..15556
                     /locus_tag="VM841_15885"
     CDS             15038..15556
                     /locus_tag="VM841_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVL91702.1"
                     /translation="MRKLITVGALLAAVALSASGASAAVTFDQITGTGFVGKGDVQLV
                     YGWNNHQLQANAANVSFSYSSEDTYSAVCTFTTGEGTRGERIHNISHKRTAQVNNTVE
                     RELRKNKLGDVTGFTLTGYGAQSSSGSVPVLGGPCQGNSGHGGEWTSVEQTGSSGGLF
                     VHYLGGAGTQIA"
     gene            complement(15725..16648)
                     /locus_tag="VM841_15890"
     CDS             complement(15725..16648)
                     /locus_tag="VM841_15890"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HVL91703.1"
                     /translation="MKVLLAAPRGYCAGVDRAVETVERALAQFGAPVYVRKQIVHNAH
                     VVRDLESKGAIFVEELDEVPEGANAILSAHGVAPEVYEQARGRSLQTIDATCPLVTKV
                     HFEARKFASDGFDIVLIGHEGHEEVIGTMGEAPSSITLIEKPADVDSLDLPGNKVAYL
                     SQTTLSVDETNEVIAALRERFPQIQGPPREDICYATQNRQTAVKEIARRADVVLVIGS
                     QNSSNSKRLAEVARVAGAESYLIDDETEIDPAWLDGAQTVGITSGASAPEWLVDRVVD
                     WFRGRGETDVEEVRVVHEDVHFGLPKELDRA"
     gene            complement(16789..>17105)
                     /locus_tag="VM841_15895"
     CDS             complement(16789..>17105)
                     /locus_tag="VM841_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVL91704.1"
                     /translation="HQGVAFKLADMAMETDAARLLVWRAAWMARNAQPFSKAEGSMSK
                     LKAGEVAVKVTEDAIQVLGGYGYIKDFPVEKWFRDSKIYCLFEGTSEIQRLVISRALG
                     SM"
BASE COUNT         2669 a         5872 c         5857 g         2707 t
ORIGIN      
        1 gcgggccccg gcgcggcaag ctcgtcctgc agctcgtggc cgacgaggag gtgctcggcc
       61 cgcacgggac catggcactc caccagcgcg gcctcgccgg cgcggacgcg gcgatcgtgg
      121 gcgagccgac cgacttgaac gtggccgtgg ccgaacgcgg catgctctgg atcatcgccc
      181 gcaccgccgg ggtggcggcg cacggctcgc ggcccgacct cggcgtgaac gccatcgagg
      241 agatggcgcg cgcgatcccg gtcctgcgcg ccctccgcac ggggcgatct catccgctgc
      301 tgggcgagcc ctcgctgaac ttcggcaccg tcgagggcgg ctccaagatc aacatcgtcg
      361 ccgacgcgtg ccgcctggcg atggaccggc gcaccctgcc cggcgagacc gaccagggca
      421 tcatcgatca gatgcaggcg gcgttgaagg acgcgggcct gtcggtgacg ctcgagatcg
      481 tgcaccgcgc cgacccggtc gaggtcgatc ccggcgagga gatcgtgcga cgcgcggtcg
      541 aggcgcacga ggcggtgcgg ggccggccgg gtgcgatcgg cgcgatgacc ggtgcgaccg
      601 acgcgcacgt cctcgccggc acggccggga tcccgaccat catcttcggg ccgggctcgc
      661 tggatcaggc gcacaccacc ggcgagcacg ttgccataac cgatctgatc gacggcgcgc
      721 gaatctatgc ggagctgttc gacgcgtacc tgtcgggcta gagcagcgcg cgggtcatgc
      781 cgccgtccac ctggacggtg acgccggtga ggaacgcggc gggctcgccg ctcaggaacg
      841 cgaccgtcgc ggcgatctcc tccggcttgc cgagccggcg catcgggatg tcggcctcga
      901 aacttttgat gacctcgtcg aaggaaacgc cgccttggcg cgcgccgtcc tcggccaagg
      961 tgcggatgcg atcggtcagc gtgggacccg gacaaacggt gttggcggtg atgccttcgg
     1021 cggcgagctc gttggcgagc gtcttcatga acccggcgac gccggcgcgc gccgtgttcg
     1081 acagcaccag accttgcagc ggctgcttga tcgccaccga ctgcaccgtc acgatgcggc
     1141 cccagcgctg cgcgcgcatg tgcggaaccg cagcggtgca cagccgcacc acgctcatca
     1201 ggtccagctc gagcgcgcgc ttgtaggcgt cgtcgtcgaa ggcgtcgaag ggaccgggcg
     1261 gcgggccgcc ggcgatcggc acgagaatgt cgaggcggcc gaactcgtcg gccgctcctt
     1321 ccacgacgcg ccggcagtcc tccggtttgg agacgtccgc gacggtcgcg cgcacgcgca
     1381 cgccgagctt cgacagctcg gattcggttt cgcgaagcgt gtcctcgccg cgcgcgcaga
     1441 tggccacgtc gcagccttcg ctcgcgagcg cgaacgccgc ggcacggccg atgcccttgc
     1501 tcgaggcggt gatcagtgcg gcgcgtccgc gcaggccgag gtccatgacc ggcgattgta
     1561 acggcgcttc gatacgatga ccgttcaagg aaggagcggc caatgggcaa gcccgagaaa
     1621 tacgtctacg tgtgcaccaa cgagcggcct gaagggcatc cgaaaggatc gtgcgtccag
     1681 cgcggctgcc tcgacgtttt ccaggagctt cgtgaccagc agggcgagaa ggccctcacg
     1741 cacttcaagg tcgtggcgac cggatgcctc gagccgtgcc tggcggggcc cacgctctac
     1801 atcgctcccg acgacgtctg gtacggcggc gtcaccgtcc aggacgtgga gcagatcatc
     1861 gaccagcacc tggtcggcgg aaagcccgtg gacttcctca cgctcaccga cgacgatttc
     1921 gagcgcagcc agcgcgccgc caagctcgcg ccgccctcga tgtagccctc ccccgagcga
     1981 gggatcggcg ctgctccgtg gccggagtgc gtcccgcgac gcgctccccc tagaatgagc
     2041 ctccatgacc gagaccgtgc cggagaccac caccgtcgag cggccgtacg gccagtggaa
     2101 agagcttttc cacgaggtcg tcacgcccaa ctactgcacc ggatgcgccg catgcgtgat
     2161 ggcctgtccg cgtgacgtgc tcggctacga ccactcgaag gcctacaagc cgttcaacat
     2221 cgaggattcg acgcccatcg acgactgcgt ccacggccag cgcggatgcg acatctgcac
     2281 gaaagcctgt ccgcgctttc gcgtctggga agtcgaatgc gacgaggcgc tgttcggccg
     2341 ggcgcgccgt cccgacgagg tgttcggcat ctacaaggag atcctgctcg tccgcgcgag
     2401 caaccccgac atcttcaagg ccggccagga cggcggcctg gtatcggcgc ttctgatcta
     2461 cgggctcgag aacgacctga tcgacggcgc attggtttcg aagttcagcg atgagcgtgc
     2521 cttcgacgtc gtgcccgggc tggtcaccga tcgcgaaggc gtgctcgcga cggcgggctc
     2581 gcgttacacc tactcggcga acccgctcgc gatgaaagaa gccgaggcgt tgaagctgaa
     2641 gaatctggcg ctggtcggca tgagctgcca ggcctcgatc aacggcacgc tcaccgcgcg
     2701 caacgtcgcc aagtacaagc ggcggatcgc gctcaccatc gggctgctgt gctcgaagtc
     2761 gttcgactac gacgggctga tggtcggcaa ggtgcagaac gagctcggca tccctctgga
     2821 ggacgtcgcc aaggtcaacg tcaaaggcaa gttccagatc tggcgcaaat ccaccggcga
     2881 gcagatcgac gtgccgctga aggaatgcca tccgttcacg cgcgaaggct gcaagctgtg
     2941 tcccgacttc gcggccgagc acgccgacat ctcgaccggc ggcctgggcc agtcggacgg
     3001 gtggacgctc acgatcgtcc ggaccgagcg cggcgccgaa tggatgcgcg cgctcaccga
     3061 cgcgggctgg atcgagaccc ggcccggcga agaagacccc ggcgcgatcg cgctgatggg
     3121 caagctggcg gccaagcagc gcaaacgctg gacggccggc ggagaccggg ccgcgcccgg
     3181 cgtgcttcca ccagaagacg gcaaccttta ggaggaaccg atgtccgatc cgatggtcct
     3241 tacctacgaa gagcgtggcg gcggccccgc cttggtcctg atccacggct tcccgctcaa
     3301 ctcctcgatg tggatcgagc agctcaaggg gctcgcgaag gtgcgcacag tcatcgccgt
     3361 gaacctgcgc ggccacggct acacgcaggt ggactccgcc gacggcttct cgatggatct
     3421 gttcgccgac gacgtcgccg cgacgctgga cgacatcggc gtggacaagg ccgacatctg
     3481 cgggctgtcg atgggcggct acgtcgcgat ggcgttctgg cggcggcaca aggacaaggt
     3541 ccgttcgatg atcttcgccg acaccaagcc cgaagccgac tccgacgaag ccaaagccgg
     3601 ccgcgagaaa accgccgccg cggtgcgcga gcaaggcatc cagccgctgg tggacggggt
     3661 gcttccgaag atgttcggcc cgaacccgtc cgaggagctc gtggcggcca cgcgcgcgat
     3721 gttcgagtcc acaccgcccg aggtcgcggc cgcggacctg ctcgcgatgc gcgaccggcc
     3781 ggactcggac ctgtcggtca tcgacgtgcc gtgcctgtgg atccacggcg agggcgacca
     3841 gatcatgccg atcgacgcgg cccgcactgc cgcgggcacg atcccggact gcaccttcgt
     3901 cgcggtcccg gacgccggtc acatgtcgcc gctggagaat cccggcgtgg tcatcccggc
     3961 gatgacggaa ttccttcaga aggtcggcaa gtagcgcggc tcgcaaaata agaagcggcc
     4021 ggtggtcgcc caccggccgc tgacttctcg cttcgaagga cctcgggaat cagcctgctt
     4081 gccagttcgc gacgacgggg tggtcgacgc cctggggacc cgccttggcg gcgccgccgg
     4141 gagacccgag accggtctgg tcgaaccact ccttgttcac cggggtgaac tgcttccaca
     4201 tgtcgggcac gtcgtcttcg tagaagatcg cctccacggg gcaggcgggc tcgcaggcgc
     4261 cgcagtcgac gcactcgtcc ggctggatgt agagcatccg cggaccctcg tagatgcagt
     4321 cgacggggca ctcttcgata caggccttgt ccttcacgtc gatgcaaggc tccgcgatcg
     4381 tgtaggtcac agcgggtact ccctccggtt cgtcgtttcg gtttagcgtc tgaatagcat
     4441 tctacctggg gagcgtcaaa acgatgcgct acgagcacac gaatcgcttg ggctgcgggg
     4501 acgtcggacg ccgcgtcacc gtccggtcac ggaccgcgga cggcttcatg accgatgccg
     4561 tgggggtcct ggaggaatgc gacgaccgtt cgttcgtgat tcgaaccaaa cggggggatt
     4621 tgatccgggt cgagcgggcc ctggtggtcg ccgccaagac catccccgac gcccccggcc
     4681 ggcccgggag ccgcggcgcg accggggcag acgacaccgg ggcagacgac accggggcag
     4741 ggcccgtgta agaaacgggt cgcttctgcg ttgttccctg tgacatcgag agcccggccg
     4801 tcgcccccgg ccgacttttg ggtcgcccgc cagcatatgt ccagggaggg gtcgtgcccg
     4861 tacgtagcgt cgcgttcccg agccacgacg gcgttcggct ggaaggccgg cttcatcttc
     4921 cccagacggc tccgcgaggc gttgccgtcc tctgccaccc cccaaccccg gccgacggcc
     4981 ttatgcccaa cgtcttgatg ccctcgctca cccgcgccct ggacacggcc ggctgggcct
     5041 cgctgcgctt caactatcgc ggcatcggcc gcagccaggg cgcgccggcc tccgcgcgca
     5101 gcgagctcga cgacgtccgg ggcgcgatcg cgtgggccga ggatgccgtc gcgaccgacg
     5161 tcacggccgt ggtgggctgg tcctcgggcg cgaccatagc gctgtgggcc tgcaccggca
     5221 gcctgtcggt cgatgtgttc gtaggcctgt caccgcccgt gtcggtctcc ggccccggga
     5281 tcccgtcgct tcccgagccg cagaggctcg ccgactggac gacccgcacg ctgctggtgt
     5341 gcgggtcggc cgatcccgcc tgcgacgagc gggacgcggc cgagctcgcg aagatgctcg
     5401 gcggcgagat ggagctggtg ccgggcgcgg accacgcctt caacgggcgc ctcgccgaga
     5461 tgagcgcgct cgtgacctgg ttcttgcgct agccagcctc cttgcggcgc cggcggccgc
     5521 gctgtaggga atgcgccaga tggaggcacg tgcggttggt ttctcgtccg gggccggccc
     5581 cgagctggag gggcggcttg cgatccccga cggcccccgc ggcgtcgcgg ttctgtgcca
     5641 tgcctatccc cccatgggcg gctcgatgtc caacgcgatc ctgccggcgc ttcaacgcgc
     5701 gctgtacaag gcgcagtggg cgtcgctgcg attcaacttc cgcggcgtgg gccgcagcga
     5761 aggcagcttc gacgggggtc gcggcgagat cgaagacgtc gcggccgcgc tggcgttcgt
     5821 gcgcgccgag gcgcccggcg cgaccgtcgc ggtcgcggga tggtccttcg gctcggtcgt
     5881 cggcctcgtg gccgcggttc gcgacggcga cgtcgccgcc tacgcgggta tcgcgccgcc
     5941 ggtgtcggcc ggctcgcgca tcgagcttcc cgcgcttccc gcccccgagg ccgcaggatc
     6001 tcttcggacc ctgttcgtct gcggcacgca ggatccggtt tccctccccg cggacgtcga
     6061 gcgcctcgcg cagcgatacg gcggagcgtc ggtggtgatc gatggagcgg gccacttctt
     6121 cgacggccgc cacgacgtgc tcgccgggac ggtgctgcgt catctcgaga gcgaatgacg
     6181 aaggcgcgcc ggcgccgggg gggctaacgc gcgaagaaca ggaagcggat cgacacggcg
     6241 agcatcagcg ctccgaagcc acgcatcaac atcacgttcg agatcttgcc ggccaaacgc
     6301 gcgccgaaga aggcgccgat cgcaaggccg atgcccagcc cgatcgcgta agggacgcgg
     6361 atctcgtggc ggcgggcgta ctcgataacg ccgaagatcg ccacgggcgc gaccagcgcc
     6421 gcgagcgagg tgccggacgc ctcgcgctgc gtgagcccga gcagcaccag cgccggcacc
     6481 atgacgatgc cgccaccgac gccgaacagg ccggagagca ccccggccaa aagaccgata
     6541 ccgatgccga tcaagatcgc cgtcatcgcc aggccagcat atcgggagaa ttcaccggat
     6601 ccctcgtgcg aggcgaccgt atgatggacg gatgcgcctc tacgacacga tggagcgggc
     6661 ggtcgtcgag atgcggcccg aacgcgagaa cacgcttacg atgtattcgt gcgggcccac
     6721 ggtgtatcgc ttcgcgcaca tcggcaacct tcgcacgttc ctgatggccg acctgatctg
     6781 ccgcgcatac cgcttccacg gcatcgaggc tcgtcagatc cagaacatca ccgacgtcgg
     6841 ccacatgacc gatgagctct tcgaccgggg cgaggacaag atgctcgtcg ccgccggtgt
     6901 cgagaacaag tccccccagg agatcgcgtc gttctacacc gcggcgttcc tcgaggacgt
     6961 ggacgcgatc ggcatccggc gcgcgatcct ctatccgaag gcatccgatc acgtcccgca
     7021 gatgatcgag atcatcgcca agctgatcga acgcgggcac gcgtatgccg tggatgggca
     7081 cgtgtactac gacaccgcgt cgtttcccgc ctacggacgc ctctcaggca acacgctcga
     7141 cgagctccag accggccatc gcaaggagat cggcgacccg aacaagcggc accactccga
     7201 cttcgtgctg tggatgcacg ccggcccgaa ccgagtcatc aagtttccgt ccccgtgggg
     7261 agacggcttt cccggatggc acatcgagtg ctccgcgatg tcgatcgagc acctgggcga
     7321 tcgcttcgac attcacaccg gcggcgagga caacgtgttc ccgcaccacg aggacgagat
     7381 cgctcagtcc gatggcgcgg ccggacatca ggtcgtgcgg cactgggtgc atggcgcgca
     7441 ccttctcagc gagggacgca agatggccaa gagcgcaggc aacttctacg acctgcgcga
     7501 cctggcggcc atgggacatg ccgagccgct cgcggtgcgg ctgctgctgc tgcagtcccg
     7561 ttaccgcgcg cagatgaact tcacgcggga cgcgctcggt gcggccgaga cgacgctggc
     7621 ccgctggcgg cgcctggtcg gcgactggtc gcgcggcgcc gcggcaccca ggtccgacct
     7681 ggagtccaaa tacgacacac gcttcagcga gacgatcgcc gacgacctgg acacgacctc
     7741 ggccgtcgcg ctggccgccg aggtcgtctc gacgccggat ctcgcgccgg gcgcgaaggc
     7801 ccggctgctc gcgcgctggg acgcgttcct gggcttgaat ctccagcgcg aggccggcga
     7861 ggcggccgcg gtgccgaccg acatcgccga gctgctcgcg cagcggcagc aggcccgagc
     7921 ggcgaaggac ttcgcggaat cggaccggct gcgggggcag attcgaggcc tcggcttcga
     7981 cgtggaggac accgccgagg gccaacgcac gcggccgatc ggctagtccc tgcgacgttt
     8041 gttggtcatt tggctccgaa tagcgaactt atatgaccaa attaacgcgc gtagatcatg
     8101 cagggaccgt attcgtcggt gtgctcccgc tcgaatcgga agccggcctt ctcggccaca
     8161 cgcaccgacg cctcgttgcc gtacatgatc agacagatga gccgggcgag ccccagccgt
     8221 tcatgcgcgt aggtcacgag cccgcgtgcc gcctcggtcg ccaagccttc gccccatcgg
     8281 tccttcgcga tcaggaacgc cacctcgacc tcttcgacgc cgtcaatcgt ccagggcagc
     8341 aggccgcatc gtccgacgaa tgcgccggtc tcgcgctcga tcgccgccca cagcccgaga
     8401 tcctggttct tcggatgccc gttcaggaag aacgtcacct cctcatgggt ctcggcgcgc
     8461 gtgaggatgc cttccgggaa gaagcgccgg atctccgggt cggcgtagag cgccgcgagc
     8521 gcgtcgaggt cgccgggctc gaaccgccgg aacacgagcc gcgccgtttc gagaacgggc
     8581 ggcaccggcg gctactcgcc gaagacgtgg aacaggacgt cgcggtcgcg cgggtcgtct
     8641 agctcgacga agatcagccg ctgccagcgg ccgagcatcg gctcgccatc ggcgaccgga
     8701 atcgcgtgcg aggtcgcgcc gaccagcatc tgcgccaagt gcgcgcggcc gttgggccgc
     8761 tcgtgctcgt cctcgatgtt ctgcgtgcgg cggtccatgt cgtcgtgcgc gtagtactcg
     8821 tccggcggca cgagcacgtc caagcggcga gcgaggtcgt ggtggacgcc gtcctcccac
     8881 tcgttgatga ccagcgcggc ggtggtgtgc tggcagtagg cgacggcaca gccgttggtg
     8941 atgccggcgt ccttgatggc gcgccgcagg tcgtcggtgc aatcgacggc accgtaccgt
     9001 tgcccggcac tgagccggat cgtgctggtc gcggacctca catctcctcc tcgttgcgtt
     9061 gcatggcgtg ggcaagcgcg ggggtctcgg cctccgggcc cgcggcgggc agggtaaacc
     9121 aaaaacgcat gcctccgccg ctgcggggtt cggcgccgat ccggccgccg tgcgcctcga
     9181 cgatggagcg ggcgatcgca agcccgagcc cggcgccccc gtcgctgcgc gagcgggagg
     9241 catcggcccg gtagaaccgc tcgaagacga gcggaatatt ttccctcgca atgcccggcc
     9301 cccggtcctc gaccatcacc ttgacgtgct tgctttcccg gtcggcgatg agggacacct
     9361 cgccgcccgc cggcgtcagg cgcacggcgt tgtcgagcag gttgaacagc acctgatgga
     9421 cccgggctcg gtctgcctgc gcggcgggca gaccggggtc gatgaggttg cgaacggcaa
     9481 cggaccggtc gcgccgggcg atcgcaacct cggcggcgac ctggccggcg accgacgcga
     9541 gcgcgaccgg ctcgaaggtc atcgcggccg ctcccgagtc cagccgcgac aggtccagaa
     9601 cctgatcgac cagggccgag agccgctcgg attgggtcag catcacctgc aaggtctcgc
     9661 ggtccggctg ctccacgccc tcgagcaggt tctcaagatg cgcgcggatc gcggcgatgg
     9721 gggtcttgag ctcgtgggag acgttggcga gcaggtcacg gcggagccgc tcgagccctt
     9781 ccaggtcggc ggccatgtgg ttgaacgcct cggcgaggcg gccgacctcg tcgcgtgaat
     9841 cggtgtggac gcgctgcgag tagtcgccgc gcgacatctg ctgcgtcgcg cgagccatgt
     9901 cgcgcagcgg cttggtcatg ccccgcgcca gcacgcgaac taacagcagc gcactcgcgc
     9961 cgctgagccc gccggccagc agcagcagcc accatgcgtc gcgcaggaac gcgcccgtgg
    10021 aatgccacgc ggccgcggcc gaggccttgc cgggaagcat gatcgccagc acgacgtaca
    10081 gcagcacgat cgtcaccacc acggccgcga tgatggccgc gcccagcttg gcgcggatcg
    10141 aggtgaagct ttgcagcggg cgggtcatac gtcccggaat gcgtagccga cgccgtgcac
    10201 ggtgcgcacg ggcgtgtcgt cggggttggc ttcggccagc tttttgcgca gccgcgcgac
    10261 gtgcgagtcc acgacgcgcc cgccggtcgc gtccacgtag ccccagaccg tgtccatgag
    10321 tttttcgcgg ttgtagacga cgccgggctc gcgcgcgagc gtgtgcagaa tgtcgaactc
    10381 gatcggggtc agcgacacct cggagccatc ggtggtgacg cgccggcgcg ccggatcgat
    10441 cgcgagcggg ccgcgctgca ccagatcgcc ggttccgccg cggctgcgtt cggcgcggcg
    10501 caggatcgcg cggacgcgat agatcagctc gcgcatgctg aacggcttgg tgagatagtc
    10561 gtcggccccg acggcgaagc cggcgatctt gtcggcctcg tcggtgcggg cggtcagcat
    10621 cagcaccggg atgggttcgc gtcgctgaat gcgcttgcag acctccagtc cgtccatgcc
    10681 gggaagcatc aggtccagca ccaccagatc cggcggatcg gcttcgcatc gctcgacggc
    10741 ggcggggccg tcgccgacga cggtggcgtc gaacccttcg gcgatcagcc gcgcgcgaac
    10801 ggcttcggcg atcgaggctt cgtcttcgac gaccagaatc gaggcggttg tcatcgccgt
    10861 ccatgctagc cccgatacgc ccgaacgtgg tcgcggcgtc gtcgcgaccg tattgcgatt
    10921 tcgttgcgac ctggggcgat acgataggag catgcccgct gccgacaaag acccgggcgt
    10981 cgagcccgat gcgcggttct cctttgcgaa tgaacgcacc ttcttggcat ggaaccgcac
    11041 cgctttggcg ttgatcgtcg cgggccttgc catcgttcaa gttttgcccg agttcgatct
    11101 gcccggtgga agccgcatca tcggcatccc gatgatcgtg ctgggcggaa cgatctcgtt
    11161 ctccagctac ggccggtggc gcgcgaacga gcaggcgctg cgcacccgca gccccctgcc
    11221 cccgtcccgc ctgccgtgga tcgtcgtggc gggaatcacc gcgggtgcgg tgcttgcgat
    11281 cgtgatcgaa gcgctggcgg gatcgggcgg atgaacgaag acgacgactt gttgtccggc
    11341 ctggcgggcg agcgaacgcg gctggcgtgg aatcgaaccg ccctggctct ggcggccaca
    11401 ggaggcgcga tcctgaaagg gctgccgtcg atccgccgcc cgcgcgacac cgtcgtcggc
    11461 attttgatcg tggcgctcgc cgggctgctg tggttcatcg gttcgctccg ctatcgaacg
    11521 cagcgcgacg cgcagcgccg gaacgtgctg gacacgcacg gcctgcggat cgtcgcggcc
    11581 ggcgtctcgg cggcagcgac gatcgcgctg gcgatcgccc tgctcccctc gcgctgatcg
    11641 cgaaactgtt gcgctttcgc ggcgtctcgg tcgcatcgct tcccaccgcc cgtttcgcgg
    11701 agtagaaccc gggcatgaac ccgacctgga ctcgaacggc aacggcggcc ggcttgggca
    11761 tcgcattcga tctggccgcc cacgaacagc agctcggcgt gtcgatgccg ctgctggtcg
    11821 cggtcgcggc aacggccgtt gcggtcacca gcccccactc gcgggagcgt cttctgctgc
    11881 tgggagctgc cgtcgccgtc gcgcccgtcg ccgcgatccg ttcggacgag gccgtcaccg
    11941 caccaattct gctcgcgatc atggccttgt tcgtgctcgc cgcgaccggc ccgggggccg
    12001 gcgcgctgtc gctgcgcggc ttcctccgac gcggactggg cgtgatgcgc gccttgctgc
    12061 gctctccgtt gttcatcggt gcgccgattc gggtcgccgt gccggccccg ccgcgggtgc
    12121 gccgcgttct gcgcagcgcc gtgatcgccg ctcctctcgt tgccgttttc gcgctgctgt
    12181 tcgcgtcggc ggatcgcgtc ttcgcgcagg cactgcttcc cgatctcgac gtccgcctcg
    12241 acgcgctggc cgggcacatc acgctgattg cgatgggcac gtggatggcc ggcgcctggt
    12301 ggccggccga agcgcagacc gccggcgcga ttgccgctcg cgatccggcc gccgtccgcc
    12361 cgccccggct ctcaccggcc gaatggagca ccgcgctggg cgcgctgacg gccctgttca
    12421 ccgtgttcgt cgccgtccag ttcgcgtttc ttttcggcgg ccgcgcccgc gtcgaagtca
    12481 caccggggct aacctatgcc gagtatgcgc gctccggctt cttccagctg ctcgcggccg
    12541 gcggattgac ggcgatggtc ttgcttggtg cttgggacca cggccggcgc gacgggcgac
    12601 gcgacgagcg gcgcttcctc ggactggccg ccgcgctgac ggcgctgtgc ctggtagtgc
    12661 tggcgtcggc ggcgctgcgg ctcgcgctgt atcagcaggc gttcggattc acgatcagcc
    12721 ggctggcggc gtggctggcg atcggagtga cgggtgccgc gttgtgcggc ctgctcgtcg
    12781 cgatcgcaac gcgacggcgg cgcaacgtcc tgagcggcgc cctcgcgatc gggctcggcg
    12841 cgctgatcgt gtgggccggg atctctcctg cggccttcgt tgcccgcaag aacatcgagc
    12901 gctatcaccg gacgggaacg atcgacatcg ggtatctcgc cacgctcgga cccgaggcga
    12961 caccggcgct cgtcgcattc gtggcgtctg cgcctgccac cgtcactgaa ccgcttcgat
    13021 atgcgctgtg ctcgttcgcg cccgagcggc gcggcgactg gcgatcggcg aacctggcgc
    13081 gcagccgcgc ggcggccgcg cgggcgacgg tgcgctgccc tgcctagcgg gtcagggctt
    13141 gagcacgact ttgagcgcct cgcgggattc gaacagctcg tagccgcgca atccttcgtc
    13201 cagcggcagc gtgtgggtga tgatcacggt cgggtcgatc acgccctgct gaaccagttg
    13261 cagcgagcgc gtccagtggc cctgcacgtt ggcgctgcct ccgaactcca ggcggatcgc
    13321 gcggcgaaat acctcgccca gcggaaagtc ccacgacaag tcggtgtaga cgccgatgac
    13381 ggcgacggtt ccgcccggac gcacggcgtt gagcgccgtg gtcagcgccg gcgtggcgcc
    13441 gacggcttcg atcaccacgt cgacgcctcg ctcgccggtt tcgcgctgca aggcgacgac
    13501 cggattgacc ttggtcatgt cgatcggggt cgcgccgagc gactcggcga gcttgaggcg
    13561 gtcttcggcc tggtccacga cgtagacgcg tgacgggttg taggtcatgg cgtgcatcag
    13621 cgagaacagt ccgaccgggc ccgcgccggt gatcgcgacc gtgtcgccct cttggatctc
    13681 ggcgcgcgac gtgatgtagc cggcggtgga caagatgtcg ccggcgaaga tcgcctgctc
    13741 gtcggtcatg cccggatcga tcgcgtgcag attcagatcg gccttgggga tgcgcacgta
    13801 ctcggcctgc gcgccgtcga ggtcgccgac gaacatcccg tagcccagaa cccacatgtc
    13861 ctcgcacagg ttgtagtcgc cgcgttcgca cgcccagcat gcaccgcacg cgatcatgaa
    13921 cgagccgacg acccggtccc ccgccttgaa gcgcttgacg cccgcaccca ccgattcgac
    13981 gatcccgacg aactcgtggc cgatcacact gcccggacgc agcccgggga tcttgccgtg
    14041 cagcagatgc aggtccgatc cgcagatcgc tgcggtcgtg atcttgatca cggcgtcacc
    14101 gggatcctgc acggacggtt cgggatagtc cccgaacgag atcaaaccga cgtcgtcgaa
    14161 caggatcgct cgcatacccg ccccccgtgt gtttcggacg tgtcgaagga tacggcggca
    14221 gcgacgcgcg gcgctaggcc gcggcaaccg ggcacgccca cccgaacacc gggtgagggg
    14281 cgaagtcgaa gcgcggcggc ggcgcggcga gccactggtg aaacagggcg atggcggagg
    14341 tccccttcga gccgcgcgtt cgcatccggc ggcggcgtct cgtagcattc gccccatgcc
    14401 ttcactcgac ttcgcattcc tggccgacgc ggccgaggcc gagcccggcc gcaagttcta
    14461 cgtgctgggc ggcggcatcg accagatcgg cggacagtcc ttcccgctgg tccaccccca
    14521 catggcactg gtgatgcgct gggtcatcaa cccgaccgag atgaacctgc gccaccatct
    14581 ggaagttcgc ctggtggacg ccgacggcca gcagctcgcc aagatcgaag gcgacatcga
    14641 ggcgtccggg ggcgccccga tgggccgcga ggcgggcgtg aacatggtca tcaatctgac
    14701 caacacccgg ttcgagaagc ccggggattt cgcattcgag gtgctgatga acaatcagca
    14761 cttcaagtcg ctgggcctgc gcgtcgccac cgtcccggtc gcctgaccgg ccgaaactta
    14821 agagcgcctt tggcgggcct gaaatcaatc tgcgccgcca ttaagggtta attatgcacg
    14881 ctgacacata gtccaacgca ctggtagaaa gggctcaatt cgagtcgtca cctggggcga
    14941 tgcaggaccg ttcctgcatc gacggccggg aaacagccgg ccgggacgac cagaatcgga
    15001 atcgcgcccg ccgggcgcga gaagggagcc ctgcttcatg agaaagctca tcaccgtcgg
    15061 cgcgctgctt gccgccgtgg cactgtcggc ctccggcgcc tcggccgccg tcacgttcga
    15121 ccagatcacc ggcaccggct tcgtcggcaa gggtgacgtc cagttggtct acggatggaa
    15181 caaccaccag ctgcaggcca acgccgcaaa cgtcagcttc agctactcct ccgaggacac
    15241 gtactcggcc gtctgcacct tcaccaccgg tgaagggacc cgcggcgagc gcatccacaa
    15301 catctcccac aagcgcaccg ctcaggtcaa caacacggtc gagcgcgagc tgcgcaagaa
    15361 caagctgggc gatgtgaccg gcttcacgct caccggctac ggcgcgcaga gctcgagcgg
    15421 ttccgtcccg gttctgggcg gcccgtgcca gggcaacagc ggtcacggcg gcgagtggac
    15481 cagcgtcgag cagaccggct cgtcgggcgg cctgttcgtg cactacctgg ggggcgcggg
    15541 aacgcagatc gcctagttat cgacagttcc atgaagaagc cggcccgcgg gccggcttct
    15601 tcatgaacgg ggtcagggac gcaggggagc gtccctgacc tgaagggcat tcccccagag
    15661 gacttccgga atgttctacg gtgcgcggga tcgtccggct gcgtcgggag agaattcttt
    15721 cgggctacgc gcggtcgagt tctttgggca ggccgaaatg cacgtcctcg tgcacgaccc
    15781 gcacctcttc gacgtcggtt tcgccgcgac cgcggaacca gtccacgacc cggtccacca
    15841 gccactcagg ggcgctggcg ccggacgtga tgccgacggt ttgcgcgccg tccagccacg
    15901 cggggtcgat ttcggtctcg tcgtcgatca agtaggactc ggcgccggcg acgcgagcca
    15961 cttcggccag gcgcttggag ttcgacgagt tctgcgagcc gatcaccagc accacgtcgg
    16021 cgcggcgtgc gatctccttc acggccgtct ggcggttctg ggtcgcgtag cagatgtcct
    16081 cgcgcggcgg cccttggatc tgcgggaagc gctcacgcag ggcggcgatg acctcgttgg
    16141 tctcgtccac cgacagcgtc gtctgcgaca ggtaggcgac cttgttgccg ggcaggtcca
    16201 gcgaatcgac gtccgcgggc ttttcgatca aggtgatcga ggacggggcc tcccccatcg
    16261 ttccgatgac ctcttcgtgg ccttcgtggc cgatcaggac gatgtcgaag ccgtccgagg
    16321 cgaacttacg ggcctcgaag tggaccttgg tcaccagcgg gcaggtcgcg tcgatcgtct
    16381 gcaagctgcg gccgcgcgcc tgctcgtaga cctcgggcgc cacgccgtgc gcggacagga
    16441 tcgcgttcgc gccttcggga acctcgtcga gctcttccac gaagatcgcg cccttggatt
    16501 cgaggtcgcg aaccacgtgg gcattgtgca cgatctgttt gcgcacgtag acgggcgcgc
    16561 cgaattgagc gagcgcacgc tcgacggtct caacggcacg gtccaccccg gcgcagtagc
    16621 cgcgcggcgc ggccagaagg accttcatgc ccccatcatg cccgaccggg aggccggccg
    16681 ctcgaagaag cccagcacgg cgtcgatgag gatccggggc tcttcgatta gcggtccttc
    16741 gggaacaacg tcccgcggtc ccctcggatc cgcaggtatc ccatggcgct acatcgaccc
    16801 cagggcgcgg gagatgacga ggcgctggat ctcgctggtg ccttcgaaca ggcagtagat
    16861 cttggagtcg cggaaccact tctcgaccgg gaagtccttg atgtagccgt agccgccgag
    16921 cacctgaatc gcgtcctcgg tgaccttgac cgcgacctcg cccgccttga gcttggacat
    16981 cgagccctcg gccttgctga acggctgcgc attgcgcgcc atccacgccg cgcgccacac
    17041 cagcagccgc gccgcgtcgg tttccatcgc catgtcggcg agcttgaacg ccacgccctg
    17101 gtgct
//
